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Sample records for sp nr pubens

  1. Metatranscriptomics and pyrosequencing facilitate discovery of potential viral natural enemies of the invasive Caribbean crazy ant, Nylanderia pubens.

    Science.gov (United States)

    Valles, Steven M; Oi, David H; Yu, Fahong; Tan, Xin-Xing; Buss, Eileen A

    2012-01-01

    Nylanderia pubens (Forel) is an invasive ant species that in recent years has developed into a serious nuisance problem in the Caribbean and United States. A rapidly expanding range, explosive localized population growth, and control difficulties have elevated this ant to pest status. Professional entomologists and the pest control industry in the United States are urgently trying to understand its biology and develop effective control methods. Currently, no known biological-based control agents are available for use in controlling N. pubens. Metagenomics and pyrosequencing techniques were employed to examine the transcriptome of field-collected N. pubens colonies in an effort to identify virus infections with potential to serve as control agents against this pest ant. Pyrosequencing (454-platform) of a non-normalized N. pubens expression library generated 1,306,177 raw sequence reads comprising 450 Mbp. Assembly resulted in generation of 59,017 non-redundant sequences, including 27,348 contigs and 31,669 singlets. BLAST analysis of these non-redundant sequences identified 51 of potential viral origin. Additional analyses winnowed this list of potential viruses to three that appear to replicate in N. pubens. Pyrosequencing the transcriptome of field-collected samples of N. pubens has identified at least three sequences that are likely of viral origin and, in which, N. pubens serves as host. In addition, the N. pubens transcriptome provides a genetic resource for the scientific community which is especially important at this early stage of developing a knowledgebase for this new pest.

  2. Astaxanthin Suppresses MPP+-Induced Oxidative Damage in PC12 Cells through a Sp1/NR1 Signaling Pathway

    Directory of Open Access Journals (Sweden)

    Xiaochun Chen

    2013-03-01

    Full Text Available Objective: To investigate astaxanthin (ATX neuroprotection, and its mechanism, on a 1-methyl-4-phenyl-pyridine ion (MPP+-induced cell model of Parkinson’s disease. Methods: Mature, differentiated PC12 cells treated with MPP+ were used as an in vitro cell model. The MTT assay was used to investigate cell viability after ATX treatment, and western blot analysis was used to observe Sp1 (activated transcription factor 1 and NR1 (NMDA receptor subunit 1 protein expression, real-time PCR was used to monitor Sp1 and NR1 mRNA, and cell immunofluorescence was used to determine the location of Sp1 and NR1 protein and the nuclear translocation of Sp1. Results: PC12 cell viability was significantly reduced by MPP+ treatment. The expression of Sp1 and NR1 mRNA and protein were increased compared with the control (p < 0.01. Following co-treatment with ATX and MPP+, cell viability was significantly increased, and Sp1 and NR1 mRNA and protein were decreased, compared with the MPP+ groups (p < 0.01. In addition, mithracycin A protected PC12 cells from oxidative stress caused by MPP+ by specifically inhibiting the expression of Sp1. Moreover, cell immunofluorescence revealed that ATX could suppress Sp1 nuclear transfer. Conclusion: ATX inhibited oxidative stress induced by MPP+ in PC12 cells, via the SP1/NR1 signaling pathway.

  3. Bacterial endosymbionts in field-collected samples of Trialeurodes sp. nr. abutiloneus (Hemiptera: Aleyrodidae).

    Science.gov (United States)

    Cass, Bodil N; Mozes-Daube, Netta; Iasur-Kruh, Lilach; Bondy, Elizabeth C; Kelly, Suzanne E; Hunter, Martha S; Zchori-Fein, Einat

    2014-01-01

    Facultative bacterial endosymbionts are common, influential associates of arthropods, yet their movement among host species has not been well documented. Plant-mediated transmission of Rickettsia has been shown for the whitefly Bemisia tabaci. Bemisia tabaci in USA cotton fields harbors the secondary symbionts Rickettsia and Hamiltonella, and co-occurs with Trialeurodes sp. nr. abutiloneus whiteflies. To determine whether symbionts may be shared, the microbial diversity of these whiteflies on cotton across the USA was analyzed. Trialeurodes sp. nr. abutiloneus bore Portiera, Pseudomonas, Serratia, Arsenophonus and Wolbachia. No Rickettsia or Hamiltonella were detected. These results provide no evidence for horizontal transmission of symbionts between these whitefly genera. Copyright © 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

  4. Canonical realizations of the Lie algebra sp(2n,R)

    International Nuclear Information System (INIS)

    Havlicek, M.; Lassner, W.

    1975-01-01

    The generators of the Lie algebra of the symplectic group sp(2n,R) are, rezcurently, realied by means of polynomials in the quantum canonical variables qsub(i) and psub(i), i=1,...,d(2n-d);d=1,...,n. These realisations are skew-hermitean, the Casimir operators are realised by constant multiples of identity element and they depend on d free real parameters

  5. Comparison of patients with ankylosing spondylitis (AS) and non-radiographic axial spondyloarthritis (nr-axSpA) from a single rheumatology clinic in New Delhi.

    Science.gov (United States)

    Malaviya, Anand N; Kalyani, Alok; Rawat, Roopa; Gogia, Shashi Bhushan

    2015-09-01

    Comparison of ankylosing spondylitis (AS) with non-radiographic axial spondyloarthritis (nr-axSpA) classified with the recent ASsessment of spondyloArthritis International Society (ASAS) criteria. This study included 288 patients clinically diagnosed as having spondyloarthritis (SpA) where a satisfactory radiograph of sacroiliac (S-I) joints was available. The AS and the nr-axSpA groups were compared for the various SpA-related variables. Of 288 axSpA patients, there were 187 with AS. Of the remaining 101 patients without radiographic sacroiliitis, S-I joint magnetic resonance imaging (MRI) was available in 72; 54 of them showed active sacroiliitis thus classified as nr-axSpA according to the ASAS criteria. The remaining 18 patients with normal MRI and the other 29 patients without MRI of the S-I joints (total 47 patients), were classified as nr-axSpA using the 'clinical arm' of the ASAS criteria. On comparing the 187 AS with 101 patients in the nr-axSpA group, the AS group showed significantly more males, longer disease duration, more axial symptoms at disease onset, higher Bath Ankylosing Spondylitis Metrology Index and more syndesmophytes. Biologicals were offered significantly more often to the AS group but methotrexate as monotherapy or in combination with other disease-modifying anti-rheumatic drugs was offered more often in nr-axSpA group. There was no statistically significant difference between AS and nr-axSpA in other SpA parameters. The differences brought out between AS and nr-axSpA groups show that they may not be the same disease. A prospective long-term follow-up of large cohorts may help in clarifying if nr-axSpA is simply an early stage in the spectrum of SpA evolving into AS over time or is there inherent difference between them. © 2015 Asia Pacific League of Associations for Rheumatology and Wiley Publishing Asia Pty Ltd.

  6. Characteristics, phenotype, and transmission of Wolbachia in the sweet potato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), and its parasitoid Eretmocerus sp. nr. emiratus (Hymenoptera: Aphelinidae).

    Science.gov (United States)

    Chiel, Elad; Kelly, Suzanne E; Harris, Alexandre M; Gebiola, Marco; Li, Xianchun; Zchori-Fein, Einat; Hunter, Martha S

    2014-04-01

    Wolbachia is a common intracellular bacterial endosymbiont of insects, causing a variety of effects including reproductive manipulations such as cytoplasmic incompatibility (CI). In this study, we characterized Wolbachia in the whitefly Bemisia tabaci and in the whitefly parasitoid Eretmocerus sp. nr. emiratus. We also tested for horizontal transmission of Wolbachia between and within trophic levels, and we determined the phenotype of Wolbachia in E. sp. nr. emiratus. Using multilocus sequence typing and phylogenetic analyses, we found that B. tabaci and E. sp. nr. emiratus each harbor a different and unique strain of Wolbachia. Both strains belong to the phylogenetic supergroup B. No evidence for horizontal transmission of Wolbachia between and within trophic levels was found in our study system. Finally, crossing results were consistent with a CI phenotype; when Wolbachia-infected E. sp. nr. emiratus males mate with uninfected females, wasp progeny survival dropped significantly, and the number of females was halved. This is the first description of CI caused by Wolbachia in the economically important genus Eretmocerus. Our study underscores the expectation that horizontal transmission events occur rarely in the dynamics of secondary symbionts such as Wolbachia, and highlights the importance of understanding the effects of symbionts on the biology of natural enemies.

  7. Effect of diatomaceous earth and Trichoderma harzianum T-22 (Rifai strain KRL-AG2) on the fungus gnat Bradysia sp. nr. coprophila (Diptera: Sciaridae).

    Science.gov (United States)

    Cloyd, Raymond A; Dickinson, Amy; Kemp, Kenneth E

    2007-08-01

    This study, consisting of three experiments, was designed to assess whether diatomaceous earth, when applied to the surface of growing media, reduces adult fungus gnat Bradysia sp. nr. coprophila (Diptera: Sciaridae) emergence or inhibits the females from laying eggs; and whether fungus gnat adults are attracted to the fungus Trichoderma harzianum T-22 (Rifai strain KRL-AG2) under laboratory conditions. In the first two experiments, diatomaceous earth was applied at two different thicknesses (3.1 and 6.3 mm) and conditions (dry and moist) to the surface of a growing medium (Universal SB 300 Mix) after the growing medium had been artificially inoculated with second or third instars of fungus gnats, or before female fungus gnat adults were released into each deli squat container. In the third experiment, preparations of the fungus T. harzianum at the highest recommended label rate (0.889 kg/m3) were amended into the growing medium and processed 24, 48, or 72 h before use in a series of three two-choice trials with a two-armed experimental arena. In the first two experiments, the dry or moist layers of diatomaceous earth, in general, did not affect fungus gnats in terms of preventing adult emergence or egg laying by the females. During the course of these experiments, we observed that the diatomaceous earth dry treatments expanded as a result of absorbing moisture from the growing medium, creating fissures that allowed the fungus gnat larvae to pupate and females to lay eggs. In the third experiment, fungus gnat adults were not attracted to the T. harzianum treatments in any of the trials.

  8. sp

    African Journals Online (AJOL)

    Vihar

    adopted as the first line drug. SP has few untoward effects if used carefully in therapeutic doses. Nausea, vomiting, generalized body weakness; diarrhea, skin rashes and hematological reactions are some of the associated side effects. The drug can cause severe skin reactions such as Steven Johnson's syndrome. This.

  9. Effects of NR1 splicing on NR1/NR3B-type excitatory glycine receptors

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    Orth Angela

    2009-04-01

    Full Text Available Abstract Background N-methyl-D-aspartate receptors (NMDARs are the most complex of ionotropic glutamate receptors (iGluRs. Subunits of this subfamily assemble into heteromers, which – depending on the subunit combination – may display very different pharmacological and electrophysiological properties. The least studied members of the NMDAR family, the NR3 subunits, have been reported to assemble with NR1 to form excitatory glycine receptors in heterologous expression systems. The heterogeneity of NMDARs in vivo is in part conferred to the receptors by splicing of the NR1 subunit, especially with regard to proton sensitivity. Results Here, we have investigated whether the NR3B subunit is capable of assembly with each of the eight functional NR1 splice variants, and whether the resulting receptors share the unique functional properties described for NR1-1a/NR3. We provide evidence that functional excitatory glycine receptors formed regardless of the NR1 isoform, and their pharmacological profile matched the one reported for NR1-1a/NR3: glycine alone fully activated the receptors, which were insensitive to glutamate and block by Mg2+. Surprisingly, amplitudes of agonist-induced currents showed little dependency on the C-terminally spliced NR1 variants in NR1/NR3B diheteromers. Even more strikingly, NR3B conferred proton sensitivity also to receptors containing NR1b variants – possibly via disturbing the "proton shield" of NR1b splice variants. Conclusion While functional assembly could be demonstrated for all combinations, not all of the specific interactions seen for NR1 isoforms with coexpressed NR2 subunits could be corroborated for NR1 assembly with NR3. Rather, NR3 abates trafficking effects mediated by the NR1 C terminus as well as the N-terminally mediated proton insensitivity. Thus, this study establishes that NR3B overrides important NR1 splice variant-specific receptor properties in NR1/NR3B excitatory glycine receptors.

  10. Distribution, Pest Status and Fungal Associates of Euwallacea nr. fornicatus in Florida Avocado Groves

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    Daniel Carrillo

    2016-10-01

    Full Text Available Members of a complex of cryptic species, that correspond morphologically to the ambrosia beetle Euwallacea fornicatus (Eichhoff (Coleoptera: Curculionidae: Scolytinae, were recently found attacking avocado (Persea americana Mill. in Israel and California. In early 2016, an outbreak of another member of this species complex was detected infesting approximately 1500 avocado trees in an avocado orchard at Homestead, Florida. An area-wide survey was conducted in commercial avocado groves of Miami-Dade County, Florida to determine the distribution and abundance of E. nr. fornicatus, to identify different populations of E. nr. fornicatus and their fungal associates, and to assess the extent of damage to avocado trees. Ewallacea nr. fornicatus were captured in 31 of the 33 sampled sites. A sample of 35 beetles from six different locations was identified as E. nr. fornicatus sp. #2, which is genetically distinct from the species causing damage in California and Israel. Eleven fungal associates were identified: an unknown Fusarium sp., AF-8, AF-6, Graphium euwallaceae, Acremonium sp. Acremonium morum, Acremonium masseei, Elaphocordyceps sp. and three yeast species. The unknown Fusarium isolates were the most abundant and frequently found fungus species associated with adult beetles and lesions surrounding the beetle galleries. In addition to fungal associates, three bacteria species were found associated with adult E. nr. fornicatus. Visual inspections detected significant damage in only two orchards. A large number of beetles were captured in locations with no apparent damage on the avocado trees suggesting that E. nr. fornicatus are associated with other host(s outside the groves or with dead trees or branches inside the groves. More research is needed to determine the potential threat E. nr. fornicatus and its fungal associates pose to the avocado industry and agricultural and natural ecosystems in Florida.

  11. A FUNCTIONAL RELATIONSHIP BETWEEN TRIGEMINAL ASTROGLIAL ACTIVATION AND NR1 EXPRESSION IN A RAT MODEL OF TEMPOROMANDIBULAR JOINT INFLAMMATION

    Science.gov (United States)

    Wang, Shuxing; Song, Li; Tan, Yonghui; Ma, Yuxin; Tian, Yinghong; Jin, Xu; Lim, Grewo; Zhang, Shuzhuo; Chen, Lucy; Mao, Jianren

    2012-01-01

    Objective To examine the hypothesis that glial activation would regulate the expression of the NR1 subunit of the N-methyl-D-aspartate receptor in the trigeminal subnucleus caudalis (Sp5C) after temporomandibular joint (TMJ) inflammation. Methods Inflammation of temporomandibular joint (TMJ) was produced in rats by injecting 50μl complete Freund's adjuvant (CFA) into unilateral TMJ space. Sham control rats received incomplete Freund's adjuvant (IFA) injection. Mechanical nociception in the affected and non-affected TMJ site was tested by using a digital algometer. Fractalkine, fluorocitrate, and/or MK801 were intracisternally administrated to examine the relationship between astroglial activation and NR1 upregulation. Results CFA TMJ injection resulted in persistent ipsilateral mechanical hyperalgesia 1, 3 and 5 days after CFA injection. The inflammation also induced significant upregulation of CX3CR1 and GFAP beginning on day 1, and of NR1 beginning on day 3, within the ipsilateral Sp5C. Intracisternal administration of fluorocitrate for 5 days blocked the development of mechanical hyperalgesia as well as the upregulation of GFAP and NR1 in the Sp5C. Conversely, intracisternal injection of fractalkine for 5 days exacerbated the expression of NR1 in Sp5C and mechanical hyperalgesia induced by TMJ inflammation. Moreover, once daily intracisternal fractalkine administration for five days in naïve rats induced the upregulation of NR1 and mechanical hyperalgesia. Conclusions These results suggest that astroglial activation contributes to the mechanism of TMJ pain through the regulation of NR1 expression in Sp5C. PMID:23110394

  12. Listeria floridensis sp. nov., Listeria aquatica sp. nov., Listeria cornellensis sp. nov., Listeria riparia sp. nov. and Listeria grandensis sp. nov., from agricultural and natural environments.

    Science.gov (United States)

    den Bakker, Henk C; Warchocki, Steven; Wright, Emily M; Allred, Adam F; Ahlstrom, Christina; Manuel, Clyde S; Stasiewicz, Matthew J; Burrell, Angela; Roof, Sherry; Strawn, Laura K; Fortes, Esther; Nightingale, Kendra K; Kephart, Daniel; Wiedmann, Martin

    2014-06-01

    Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded 18 Listeria-like isolates that could not be assigned to previously described species using traditional methods. Using whole-genome sequencing and traditional phenotypic methods, we identified five novel species, each with a genome-wide average BLAST nucleotide identity (ANIb) of less than 85% to currently described species. Phylogenetic analysis based on 16S rRNA gene sequences and amino acid sequences of 31 conserved loci showed the existence of four well-supported clades within the genus Listeria; (i) a clade representing Listeria monocytogenes, L. marthii, L. innocua, L. welshimeri, L. seeligeri and L. ivanovii, which we refer to as Listeria sensu stricto, (ii) a clade consisting of Listeria fleischmannii and two newly described species, Listeria aquatica sp. nov. (type strain FSL S10-1188(T) = DSM 26686(T) = LMG 28120(T) = BEI NR-42633(T)) and Listeria floridensis sp. nov. (type strain FSL S10-1187(T) = DSM 26687(T) = LMG 28121(T) = BEI NR-42632(T)), (iii) a clade consisting of Listeria rocourtiae, L. weihenstephanensis and three novel species, Listeria cornellensis sp. nov. (type strain TTU A1-0210(T) = FSL F6-0969(T) = DSM 26689(T) = LMG 28123(T) = BEI NR-42630(T)), Listeria grandensis sp. nov. (type strain TTU A1-0212(T) = FSL F6-0971(T) = DSM 26688(T) = LMG 28122(T) = BEI NR-42631(T)) and Listeria riparia sp. nov. (type strain FSL S10-1204(T) = DSM 26685(T) = LMG 28119(T) = BEI NR- 42634(T)) and (iv) a clade containing Listeria grayi. Genomic and phenotypic data suggest that the novel species are non-pathogenic. © 2014 IUMS.

  13. Mediernes ret til at offentliggøre billeder af offentligt kendte personer efter von Hannover nr. 2

    DEFF Research Database (Denmark)

    Riis, Thomas; Udsen, Henrik; Borberg, Vibeke

    2015-01-01

    Artiklen er baseret på en analyse af den Europæiske Menneskerettighedsdomstols dom i den anden sag vedrørende prinsesse Caroline von Hannover af Monaco, som gennem mange år har forsøgt at beskytte sit privatliv mod eksponering i ugebladspressen (von Hannover nr. 2). Artiklen behandler også spørgs...

  14. SP (4,R) symmetry in light nuclei

    International Nuclear Information System (INIS)

    Peterson, D.R.

    1979-01-01

    A classification of nuclear states according to the noncompact sympletic Lie algebras sp(2n,R), n = 1, 2, 3, is investigated. Such a classification has recently been shown to be physically meaningful. This classification scheme is the appropriate generalization fo Elliott's SU 3 model of rotational states in deformed light nuclei to include core excitations. A restricted classification according to the Lie algebra, sp(4,R), is motivated. Truncation of the model space to a single sp(4,R) irreducible representation allows the inclusion of states possessing very high excitation energy. An sp(4,R) model study is performed on S = T = 0 positive-parity rotational bands in the deformed light nuclei 16 O and 24 Mg. States are included in the model space that possess up to 10h ω in excitation energy. Results for the B(E2) transition rates compare favorable with experiment, without resort to effective charges

  15. Spinal cord NR1 serine phosphorylation and NR2B subunit suppression following peripheral inflammation

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    Del Valle-Pinero Arseima Y

    2005-09-01

    Full Text Available Abstract Background Spinal cord N-methyl-D-aspartate (NMDA receptors are intimately involved in the development and maintenance of central sensitization. However, the mechanisms mediating the altered function of the NMDA receptors are not well understood. In this study the role of phosphorylation of NR1 splice variants and NR2 subunits was examined following hind paw inflammation in rats. We further examined the level of expression of these proteins following the injury. Results Lumbar spinal cord NR1 subunits were found to be phosphorylated on serine residues within two hours of the induction of hind paw inflammation with carrageenan. The enhanced NR1 serine phosphorylation reversed within six hours. No phosphorylation on NR1 threonine or tyrosine residues was observed. Likewise, no NR2 subunit phosphorylation was observed on serine, threonine or tyrosine residues. An analysis of NR1 and NR2 protein expression demonstrated no change in the levels of NR1 splice variants or NR2A following the inflammation. However, spinal cord NR2B expression was depressed by the hind paw inflammation. The expression of NR2B remained depressed for more than one week following initiation of the inflammation. Conclusion These data suggest that NR1 serine phosphorylation leads to an initial increase in NMDA receptor activity in the spinal cord following peripheral injury. The suppression of NR2B expression suggests compensation for the enhanced nociceptive activity. These data indicate that spinal cord NMDA receptors are highly dynamic in the development, maintenance and recovery from central sensitization following an injury. Thus, chronic pain therapies targeted to NMDA receptors should be designed for the exact configuration of NMDA receptor subunits and post-translational modifications present during specific stages of the disease.

  16. Cloning and phylogenetic analysis of NMDA receptor subunits NR1, NR2A and NR2B in Xenopus laevis tadpoles

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    Rebecca C Ewald

    2009-09-01

    Full Text Available N-methyl-D-aspartate receptors (NMDARs play an important role in many aspects of nervous system function such as synaptic plasticity and neuronal development. NMDARs are heteromers consisting of an obligate NR1 and most commonly one or two kinds of NR2 subunits. While the receptors have been well characterized in some vertebrate and invertebrate systems, information about NMDARs in Xenopus laevis brain is incomplete. Here we provide biochemical evidence that the NR1, NR2A and NR2B subunits of NMDARs are expressed in the central nervous system of X. laevis tadpoles. The NR1-4a/b splice variants appear to be the predominant isoforms while the NR1-3a/b variants appear to be expressed at low levels. We cloned the X. laevis NR2A and NR2B subunits and provide a detailed annotation of their functional domains in comparison with NR2A and NR2B proteins from 10 and 13 other species, respectively. Both NR2A and NR2B proteins are remarkably well conserved between species, consistent with the importance of NMDARs in nervous system function.

  17. Mis toimub "Objektis nr 2011"? / Elli Kalju

    Index Scriptorium Estoniae

    Kalju, Elli

    2011-01-01

    Tallinnas Vabaduse väljakul 27.02.-20.03.2011 paiknevast installatsioonist "Objekt nr. 2011", kus alati keegi sees istub. Kultuuritegelased vahetuvad iga tunni järel, järjekorda oodatakse Tallinna Kunsihoones asuvas staabis

  18. Chthonius (Chthonius) onaei n. sp. (Chthoniidae, pseudoscorpiones), a new epigean species from Croatia

    OpenAIRE

    Ćurčić B.P.M.; Dimitrijević R.N.; Rađa T.; Ćurčić Nina B.; Milinčić M.

    2010-01-01

    A new epigean pseudoscorpion, Chthonius (Chthonius) onaei n. sp. is erected from Podašpilje, nr. Omiš, Mt. Omiška Dinara, Dalmatia, Croatia. Its interrelations with two close congeners, Chthonius (C.) litoralis Hadži, 1933 and Chthonius (C.) dalmatinus Hadži, 1930 are briefly discussed.

  19. Chthonius (Chthonius onaei n. sp. (Chthoniidae, pseudoscorpiones, a new epigean species from Croatia

    Directory of Open Access Journals (Sweden)

    Ćurčić B.P.M.

    2010-01-01

    Full Text Available A new epigean pseudoscorpion, Chthonius (Chthonius onaei n. sp. is erected from Podašpilje, nr. Omiš, Mt. Omiška Dinara, Dalmatia, Croatia. Its interrelations with two close congeners, Chthonius (C. litoralis Hadži, 1933 and Chthonius (C. dalmatinus Hadži, 1930 are briefly discussed.

  20. Synaptic NR2A- but not NR2B-containing NMDA receptors increase with blockade of ionotropic glutamate receptors

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    Jakob Von Engelhardt

    2009-10-01

    Full Text Available NMDA receptors are key molecules involved in physiological and pathophysiological brain processes such as plasticity and excitotoxicity. Neuronal activity regulates NMDA receptor levels in the cell membrane. However, little is known on which time scale this regulation occurs and whether the two main diheteromeric NMDA receptor subtypes in forebrain, NR1/NR2A and NR1/NR2B, are regulated in a similar fashion. As these differ considerably in their electrophysiological properties, the NR2A/NR2B ratio affects the neurons’ reaction to NMDA receptor activation. Here we provide evidence that the basal turnover rate in the cell membrane of NR2A- and NR2B-containing receptors is comparable. However, the level of the NR2A subtype in the cell membrane is highly regulated by NMDA receptor activity, resulting in a several-fold increased insertion of new receptors after blocking NMDA receptors for 8 hours. Blocking AMPA receptors also increases the delivery of NR2A-containing receptors to the cell membrane. In contrast, the amount of NR2B-containing receptors in the cell membrane is not affected by ionotropic glutamate receptor block. Moreover, electrophysiological analysis of synaptic currents in hippocampal cultures and CA1 neurons of hippocampal slices revealed that after 8 hours of NMDA receptor blockade the NMDA EPSCs increase as a result of augmented NMDA receptor-mediated currents. In conclusion, synaptic NR2A- but not NR2B-containing receptors are dynamically regulated, enabling neurons to change their NR2A/NR2B ratio within a time scale of hours.

  1. Analysis list: Nr3c1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Nr3c1 Adipocyte,Blood,Breast,Embryo,Embryonic fibroblast,Liver,Neural + mm9 http://...dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Nr3c1.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Nr3c1.5.tsv http:...//dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Nr3c1.10.tsv http://dbarchive.bioscie...ncedbc.jp/kyushu-u/mm9/colo/Nr3c1.Adipocyte.tsv,http://dbarchive.biosciencedbc.jp.../kyushu-u/mm9/colo/Nr3c1.Blood.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Nr3c1.Breast.tsv,http:

  2. Potentiation of glycine-gated NR1/NR3A NMDA receptors relieves Ca2+-dependent outward rectification

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    Christian Madry

    2010-03-01

    Full Text Available Glycine has diverse functions within the mammalian central nervous system. It inhibits postsynaptic neurons via strychnine-sensitive glycine receptors (GlyRs and enhances neuronal excitation through co-activation of N-methyl-D-aspartate (NMDA receptors. Classical Ca2+-permeable NMDA receptors are composed of glycine-binding NR1 and glutamate-binding NR2 subunits, and hence require both glutamate and glycine for efficient activation. In contrast, recombinant receptors composed of NR1 and the glycine binding NR3A and/or NR3B subunits lack glutamate binding sites and can be activated by glycine alone. Therefore these receptors are also named excitatory glycine receptors. Co-application of antagonists of the NR1 glycine-binding site or of the divalent cation Zn2+ markedly enhances the glycine responses of these receptors. To gain further insight into the properties of these glycine-gated NMDA receptors, we investigated their current-voltage (I-V dependence. Whole-cell current-voltage relations of glycine currents recorded from NR1/NR3B and NR1/NR3A/NR3B expressing oocytes were found to be linear under our recording conditions. In contrast, NR1/NR3A receptors displayed a strong outwardly rectifying I-V relation. Interestingly, the voltage-dependent inward current block was abolished in the presence of NR1 antagonists, Zn2+ or a combination of both. Further analysis revealed that Ca2+ (1.8 mM present in our recording solutions was responsible for the voltage-dependent inhibition of ion flux through NR1/NR3A receptors. Since physiological concentrations of the divalent cation Mg2+ did not affect the I-V dependence, our data suggest that relief of the voltage-dependent Ca2+ block of NR1/NR3A receptors by Zn2+ may be important for the regulation of excitatory glycinergic transmission, according to the Mg2+-block of conventional NR1/NR2 NMDA receptors.

  3. SP. Pescado

    Directory of Open Access Journals (Sweden)

    Renato Gendre

    2003-12-01

    Full Text Available Nell'occhiello di un articolo dal titolo Il Peru dei de[Jini rosa e de/la grande pioggia si legge: "da una partenza  in aereo al «pescado»  che ti  sfamera."1 Questa parola spagnola, giustamente chiusa tra caporali, a noi pare molto interes­ sante, perche, nonostante l'apparenza, non ha nulla da spartire sotto i1 profilo se­ mantico con l'it. pescato. lnfatti, tutti i piu importanti dizionari della lingua italiana, di ieri e di oggi, etimologici e non 2, registrano  accanto a pescata,  ii lemma pescato, 3 ma lo spiegano come "quantita di pesce catturato nel corso di una battuta o di una stagione di pesca",4 mentre lo sp. pescado  indica i1 "pesce (solo nel senso di: pesGe pescato da mangiare [...]".s

  4. Analysis list: NR1H3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NR1H3 Adipocyte,Blood + hg19 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target.../NR1H3.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/NR1H3.5.tsv http://dbarchive.bioscienced...bc.jp/kyushu-u/hg19/target/NR1H3.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/NR1H3.Adipocyte....tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/NR1H3.Blood.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/hg19/colo/Adipocyte.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/Blood.gml ...

  5. The nuclear receptor NR2E1/TLX controls senescence

    Science.gov (United States)

    Krusche, Benjamin; Pemberton, Helen; Alonso, Marta M.; Chandler, Hollie; Brookes, Sharon; Parrinello, Simona; Peters, Gordon; Gil, Jesús

    2014-01-01

    The nuclear receptor NR2E1 (also known as TLX or tailless) controls the self-renewal of neural stem cells (NSCs) and has been implied as an oncogene which initiates brain tumours including glioblastomas. Despite NR2E1 regulating targets like p21CIP1 or PTEN we still lack a full explanation for its role in NSC self-renewal and tumorigenesis. We know that Polycomb repressive complexes (PRC) also control stem cell self-renewal and tumorigenesis, but so far, no formal connection has been established between NR2E1 and PRCs. In a screen for transcription factors regulating the expression of the Polycomb protein CBX7, we identified NR2E1 as one of its more prominent regulators. NR2E1 binds at the CBX7 promoter, inducing its expression. Notably CBX7 represses NR2E1 as part of a regulatory loop. Ectopic NR2E1 expression inhibits cellular senescence, extending cellular lifespan in fibroblasts via CBX7-mediated regulation of p16INK4a and direct repression of p21CIP1. In addition NR2E1 expression also counteracts oncogene-induced senescence (OIS). The importance of NR2E1 to restrain senescence is highlighted through the process of knocking down its expression, which causes premature senescence in human fibroblasts and epithelial cells. We also confirmed that NR2E1 regulates CBX7 and restrains senescence in NSCs. Finally, we observed that the expression of NR2E1 directly correlates with that of CBX7 in human glioblastoma multiforme. Overall we identified control of senescence and regulation of Polycomb action as two possible mechanisms that can join those so far invoked to explain the role of NR2E1 in control of NSC self-renewal and cancer. PMID:25328137

  6. Draft genome sequences of six neonatal meningitis-causing escherichia coli isolates (SP-4, SP-5, SP-13, SP-16, SP-46, and SP-65)

    Science.gov (United States)

    Neonatal meningitis Escherichia coli isolates (SP-4, SP-5, SP-13, SP-16, SP-46, and SP-65) were recovered from infants in the Netherlands from 1989 to 1997. Here, we report the draft genome sequences for these six E. coli isolates, which are currently being used to validate food safety processing te...

  7. Collection efficiency of the soot-particle aerosol mass spectrometer (SP-AMS) for internally mixed particulate black carbon

    Science.gov (United States)

    Willis, M. D.; Lee, A. K. Y.; Onasch, T. B.; Fortner, E. C.; Williams, L. R.; Lambe, A. T.; Worsnop, D. R.; Abbatt, J. P. D.

    2014-12-01

    The soot-particle aerosol mass spectrometer (SP-AMS) uses an intra-cavity infrared laser to vaporize refractory black carbon (rBC) containing particles, making the particle beam-laser beam overlap critical in determining the collection efficiency (CE) for rBC and associated non-refractory particulate matter (NR-PM). This work evaluates the ability of the SP-AMS to quantify rBC and NR-PM mass in internally mixed particles with different thicknesses of organic coating. Using apparent relative ionization efficiencies for uncoated and thickly coated rBC particles, we report measurements of SP-AMS sensitivity to NR-PM and rBC, for Regal Black, the recommended particulate calibration material. Beam width probe (BWP) measurements are used to illustrate an increase in sensitivity for highly coated particles due to narrowing of the particle beam, which enhances the CE of the SP-AMS by increasing the laser beam-particle beam overlap. Assuming complete overlap for thick coatings, we estimate CE for bare Regal Black particles of 0.6 ± 0.1, which suggests that previously measured SP-AMS sensitivities to Regal Black were underestimated by up to a factor of 2. The efficacy of the BWP measurements is highlighted by studies at a busy road in downtown Toronto and at a non-roadside location, which show particle beam widths similar to, but greater than that of bare Regal Black and coated Regal Black, respectively. Further BWP measurements at field locations will help to constrain the range of CE for fresh and aged rBC-containing particles. The ability of the SP-AMS to quantitatively assess the composition of internally mixed particles is validated through measurements of laboratory-generated organic coated particles, which demonstrate that the SP-AMS can quantify rBC and NR-PM over a wide range of particle compositions and rBC core sizes.

  8. Attraction of Euwallacea nr. fornicatus to lures containing quercivorol

    Science.gov (United States)

    Euwallacea nr. fornicatus is an exotic ambrosia beetle that vectors fungal Fusarium spp. to avocados. Two field trials testing potential attractants to trap Euwallacea spp. were conducted in south Florida. Quercivorol + Ultra High Release Ethanol (URH) was the more powerful attractant for E. nr. for...

  9. Neuronal kappa B-binding factors consist of Sp1-related proteins. Functional implications for autoregulation of N-methyl-D-aspartate receptor-1 expression.

    Science.gov (United States)

    Mao, Xianrong; Moerman, Andrea M; Barger, Steven W

    2002-11-22

    Neurons contain a protein factor capable of binding DNA elements normally bound by the transcription factor NF-kappaB. However, several lines of evidence suggest that this neuronal kappaB-binding factor (NKBF) is not bona fide NF-kappaB. We have identified NKBF from cultures of neocortical neurons as a complex containing proteins related to Sp1. This complex was bound by antibodies to Sp1, Sp3, and Sp4 and was competed from binding to an NF-kappaB element by an oligonucleotide containing an Sp1-binding site. This Sp1 oligonucleotide detected an abundant factor in neuronal nuclei that migrated in electrophoretic mobility shift assays at a position consistent with NKBF. Expression of transfected Sp1 stimulated transcription in a manner dependent upon a kappaB cis-element. Similar to our previous reports for NKBF (Mao, X., Moerman, A. M., Lucas, M. M., and Barger, S. W. (1999) J. Neurochem. 73, 1851-1858 and Moerman, A. M., Mao, X., Lucas, M. M., and Barger, S. W. (1999) Mol. Brain Res. 67, 303-315), the activity of the Sp1-related factor was reduced by activation of ionotropic glutamate receptors, consistent with proteolytic degradation of all three Sp1-related factors. Expression of the N-methyl-d-aspartate receptor-1 (NR1) subunit of glutamate receptors correlated with the activity of the Sp1-related factor, specifically through an Sp1 element in the NR1 promoter. These data provide the first evidence that Sp1 or related family members are responsible for kappaB-binding activity and are involved in a negative feedback for NR1 in central nervous system neurons.

  10. RESTAURANT Nr 1 (building 501 - Meyrin site)

    CERN Document Server

    2003-01-01

    OPENING TIMES in JANUARY-FEBRUARY 2004 Customers are kindly requested to note the modified opening times of restaurant nr. 1 and the adjoining newspaper stand from Monday, January 5 to Sunday February 29, 2004: Kiosquefrom Monday to Friday07:30 - 17:00 hrs Restaurant from Monday to FridaySaturday / Sunday 07:00 - 23:00 hrs08:00 - 21:00 hrs Hot meals will be served between 11:30 and 14:00 hrs, then from 18:00 to 19:30 hrs. Restaurant Supervisory Committee

  11. >RESTAURANT Nr 1 (building 501 - Meyrin site)

    CERN Multimedia

    2004-01-01

    Customers are kindly requested to note the modified opening times of restaurant nr. 1 and the adjoining newspaper stand from Monday, January 5 to Sunday February 29, 2004: - Kiosque from Monday to Friday 07:30 - 17:00 hrs - Restaurant from Monday to Friday Saturday / Sunday 07:00 - 23:00 hrs08:00 - 21:00 hrs Hot meals will be served between 11:30 and 14:00 hrs, then from 18:00 to 19:30 hrs. Restaurant Supervisory Committee

  12. Cloning and expression of the human N-methyl-D-aspartate receptor subunit NR3A

    DEFF Research Database (Denmark)

    Eriksson, Maria; Nilsson, Anna; Froelich-Fabre, Susanne

    2002-01-01

    Native N-methyl-D-aspartate (NMDA) receptors are heteromeric assemblies of four or five subunits. The NMDA receptor subunits, NR1, NR2A, NR2B, NR2C, and NR2D have been cloned in several species, including man. The NR3A subunit, which in rodents is predominantly expressed during early development......, seems to function by reducing the NMDA receptor response. The human homologue to the rat NR3A, however, had not been cloned. In order to study the functions of the human NR3A (hNR3A), we have cloned and sequenced the hNR3A. It was found to share 88% of the DNA sequence with the rat gene, corresponding...

  13. The USA Nr Inventory: Dominant Sources and Primary Transport Pathways

    Science.gov (United States)

    Sabo, R. D.; Clark, C.; Sobota, D. J.; Compton, J.; Cooter, E. J.; Schwede, D. B.; Bash, J. O.; Rea, A.; Dobrowolski, J. P.

    2016-12-01

    Efforts to mitigate the deleterious effects of excess reactive nitrogen (Nr) on human health and ecosystem goods and service while ensuring food, biofuel, and fiber availability, is one of the most pressing environmental management challenges of this century. Effective management of Nr requires up to date inventories that quantitatively characterize the sources, transport, and transformation of Nr through the environment. The inherent complexity of the nitrogen cycle, however, through multiple exchange points across air, water, and terrestrial media, renders such inventories difficult to compile and manage. Previous Nr Inventories are for 2002 and 2007, and used data sources that have since been improved. Thus, this recent inventory will substantially advance the methodology across many sectors of the inventory (e.g. deposition and biological fixation in crops and natural systems) and create a recent snapshot that is sorely needed for policy planning and trends analysis. Here we use a simple mass balance approach to estimate the input-output budgets for all United States Geologic Survey Hydrologic Unit Code-8 watersheds. We focus on a recent year (i.e. 2012) to update the Nr Inventory, but apply the analytical approach for multiple years where possible to assess trends through time. We also compare various sector estimates using multiple methodologies. Assembling datasets that account for new Nr inputs into watersheds (e.g., atmospheric NOy deposition, food imports, biologic N fixation) and internal fluxes of recycled Nr (e.g., manure, Nr emmissions/volatilization) provide an unprecedented, data driven computation of N flux. Input-output budgets will offer insight into 1) the dominant sources of Nr in a watershed (e.g., food imports, atmospheric N deposition, or fertilizer), 2) the primary loss pathways for Nr (e.g., crop N harvest, volatilization/emissions), and 3) what watersheds are net sources versus sinks of Nr. These insights will provide needed clarity for

  14. Identifikasi Molekuler Fitoplasma yang Berasosiasi dengan Tanaman Kaktus Hias Opuntia sp.

    Directory of Open Access Journals (Sweden)

    Ariny Prasetya

    2017-11-01

    Full Text Available Cactus species (Opuntia sp. is a popular ornamental succulent plant. Some ornamental cactus species in Indonesia showing proliferation and green mosaic pattern symptoms have been reported to be associated with phytoplasma infection. However, further molecular identification for accurate classification of the causal phytoplasma has not been done.  This study aimed to diagnose phytoplasma associated with Opuntia sp. based on molecular methods involving PCR standard combined with nested-PCR, cloning and DNA sequencing. Standard PCR was carried out using P1/P7 primers followed by nested-PCR using R16F2n/R16R2 or fU5/rU3 primer pairs which amplify the 16S rRNA gene targets of 1.2 kb and 880 bp, respectively. Amplified fragment of nested-PCR using R16F2n/R16R2 primers was chosen to be cloned and sequenced for further identification and classification of phytoplasma.  BLASTn analysis showed that the phytoplasma from Opuntia sp. was closely related to 16SrII group. Phylogenetic analysis and in silico RFLP indicated that phytoplasma strain infecting Opuntia sp. was a member of subgroup 16SrII-C (cactus witches’ broom phytoplasma. This is a newly report of cactus witches’ broom phytoplasma on Opuntia sp. in Indonesia.   

  15. Marianna Kaadi "Auk nr 8" linastub Põhja-Ameerikas

    Index Scriptorium Estoniae

    2011-01-01

    Marianna Kaadi dokumentaalfilmi "Auk nr 8" Põhja-Ameerika esilinastus toimub Full Frame filmifestivalil Durhamis, Põhja- Carolinas. Film on valitud ka Kanadas Torontos toimuva Hot Docs filmifestivali võistlusprogrammi

  16. Pressure vessels dossier restoration according to NR-13 requirements; Enquadramento de vasos de pressao a norma NR-13

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Jose L. [PETROBRAS Transporte S.A. (TRANSPETRO), Rio de Janeiro, RJ (Brazil); Goncalves, Osorio C. [PETROBRAS Transporte S.A. (TRANSPETRO), Rio de Janeiro, RJ (Brazil)

    2005-07-01

    Pressure vessels are static pressurized equipment typical in oil industry facilities. In TRANSPETRO terminals and stations as well as in the whole PETROBRAS, these equipment can be found in the form of condenser accumulators, separators, heat exchangers, storage spheres and others. Because they work sustaining pressure and, many times flammable fluids, pressure vessels have a reasonable potential for hazard. For this reason, the NR-13 regulation was created. It deals with the safety in maintenance, operation and inspection of pressure vessels and boilers. During the compliance to the NR- 13 rules, a problem usually found is the lack of documents for different reasons. In this case, the NR-13 obligates the owner to recreate the vessel documentation under the responsibility of a chartered professional. This paper presents a case study where NR-13 rules were conformed by tasks involving documentation reconstruction based on information collected by means of inspection and tests performed on the field. (author)

  17. The expression of nr0b1 and nr5a4 during gonad development and sex change in protandrous black porgy fish, Acanthopagrus schlegeli.

    Science.gov (United States)

    Wu, Guan-Chung; Tomy, Sherly; Chang, Ching-Fong

    2008-02-01

    Protandrous black porgy fish, Acanthopagrus schlegeli, have a striking life cycle, with a mono-male sex differentiation at the juvenile stage and male-to-female sex change at 3 yr of age. We report for the first time integrative molecular data on these interesting phenomena. Sex differentiation occurred between 4 and 5 mo of age. Testicular nr5a4 transcripts increased to high levels during sex differentiation (5 mo old), whereas nr0b1 (Dax-1) did not increase until the age of 8 mo. High nr5a4 and nr0b1 expression in testicular tissue, in contrast to low nr5a4 and high nr0b1 expression in ovarian tissue, were found in the male phase of 0(+)- to 2-yr-old fish (before sex change). Increased nr5a4, decreased nr0b1, and increased cyp19a1a were found in the ovarian tissues undergoing development from primary oocytes to vitellogenic oocytes during the natural sex change in 2(+)-yr-old fish. Removal of testicular tissue in 1(+)-yr-old fish resulted in both increased ovarian nr5a4 and genes in the steroidogenic pathway and decreased nr0b1 together with the appearance of vitellogenic oocytes. Ovary developed into the active stage with the increased expression of star and steroidogenic enzymes, including aromatase, in concordance with the decreased expression of nr0b1 in the testis-excised fish. Long-term estradiol (E2) administration resulted in early sex change, but the ovaries were mainly with primary oocytes. Low nr5a4, high nr0b1, and low steroidogenic enzymes, including cyp19a1a expression, were also observed in these E2-fed ovarian tissues. Thus, nr5a4 but not nr0b1 was associated with male sex differentiation. Testicular development required cooperative functions of both nr5a4 and nr0b1. The present study suggests that nr5a4 and nr0b1 have an antagonistic interaction for the oocyte development. Testicular tissue exerted inhibitory effects on ovarian development. It is probable that nr0b1 regulates the timing of vitellogenic development and sex change in black porgy.

  18. Expression of NR1 subunit of NMDA receptor and PSD-93/95 in rat hippocampus affected by NR1/NR2 antisense oligodeoxynucleotide

    Czech Academy of Sciences Publication Activity Database

    Vrajová, M.; Bubeníková-Valešová, V.; Klaschka, Jan

    2010-01-01

    Roč. 9, Suppl.1 (2010), S147 ISSN 1744-859X. [International Congress on Neurobiology and Clinical Psychopharmacology and European Psychiatric Association Conference on Treatment Guidance /1./. 19.11.2009-22.11.2009, Thessaloniki] R&D Projects: GA ČR GD309/09/H072; GA MŠk(CZ) 1M0517 Institutional research plan: CEZ:AV0Z10300504 Keywords : schizophrenia * animal model * NMDA receptor * aODN-NR1/NR2 * PPI Subject RIV: FL - Psychiatry, Sexuology

  19. ExaSP2

    Energy Technology Data Exchange (ETDEWEB)

    2017-09-08

    ExaSP2 is a reference implementation of typical linear algebra algorithms and workloads for a quantum molecular dynamics (QMD) electronic structure code. The algorithm is based on a recursive second-order Fermi-Operator expansion method (SP2) and is tailored for density functional based tight-binding calculations of material systems.

  20. Analysis of NR3A receptor subunits in human native NMDA receptors

    DEFF Research Database (Denmark)

    Nilsson, Anna; Eriksson, Maria; Muly, E Chris

    2007-01-01

    NR3A, representing the third class of NMDA receptor subunits, was first studied in rats, demonstrating ubiquitous expression in the developing central nervous system (CNS), but in the adult mainly expressed in spinal cord and some forebrain nuclei. Subsequent studies showed that rodent and non-human...... primate NR3A expression differs. We have studied the distribution of NR3A in the human CNS and show a widespread distribution of NR3A protein in adult human brain. NR3A mRNA and protein were found in all regions of the cerebral cortex, and also in the subcortical forebrain, midbrain and hindbrain. Only...... very low levels of NR3A mRNA and protein could be detected in homogenized adult human spinal cord, and in situ hybridization showed that expression was limited to ventral motoneurons. We found that NR3A is associated with NR1, NR2A and NR2B in adult human CNS, suggesting the existence of native NR1-NR2...

  1. Fuzzy SP-irresolute functions

    International Nuclear Information System (INIS)

    Abbas, S.E.

    2004-01-01

    In this paper, fuzzy SP-irresolute, fuzzy SP-irresolute open and fuzzy SP-irresolute closed functions between fuzzy topological spaces in Sostak sense are defined. Their properties and the relationships between these functions and other functions introduced previously are investigated. Next fuzzy SP-connectedness is introduced and studied with the help of r-fuzzy strongly preopen sets

  2. Co-Application of Corticosterone and Growth Hormone Upregulates NR2B Protein and Increases the NR2B:NR2A Ratio and Synaptic Transmission in the Hippocampus

    Directory of Open Access Journals (Sweden)

    Ghada S. Mahmoud

    2014-10-01

    Full Text Available Objectives: This in vitro study aimed to investigate the possible mechanism underlying the protective effect of growth hormone (GH on hippocampal function during periods of heightened glucocorticoid exposure. Methods: This study was conducted between January and June 2005 at the Joan C. Edwards School of Medicine, Marshall University, in Huntington, West Virginia, USA. The effects of the co-application of GH and corticosterone (CORT were tested at different concentrations on the field excitatory postsynaptic potentials (fEPSPs of the hippocampal slices of rats in two different age groups. Changes in the protein expression of N-methyl-D-aspartate receptor (NMDAR subunits NR1, NR2B and NR2A were measured in hippocampal brain slices treated with either artificial cerebrospinal fluid (ACSF, low doses of CORT alone or both CORT and GH for three hours. Results: The co-application of CORT and GH was found to have an additive effect on hippocampal synaptic transmission compared to either drug alone. Furthermore, the combined use of low concentrations of GH and CORT was found to have significantly higher effects on the enhancement of fEPSPs in older rats compared to young ones. Both GH and CORT enhanced the protein expression of the NR2A subunit. Simultaneous exposure to low concentrations of GH and CORT significantly enhanced NR2B expression and increased the NR2B:NR2A ratio. In contrast, perfusion with CORT alone caused significant suppression in the NR1 and NR2B protein expression and a decrease in the NR2B:NR2A ratio. Conclusion: These results suggest that NMDARs provide a potential target for mediating the GH potential protective effect against stress and age-related memory and cognitive impairment.

  3. Molecular mechanism of ligand recognition by NR3 subtype glutamate receptors

    Energy Technology Data Exchange (ETDEWEB)

    Yao, Yongneng; Harrison, Chris B.; Freddolino, Peter L.; Schulten, Klaus; Mayer, Mark L. (UIUC); (NIH)

    2008-10-27

    NR3 subtype glutamate receptors have a unique developmental expression profile, but are the least well-characterized members of the NMDA receptor gene family, which have key roles in synaptic plasticity and brain development. Using ligand binding assays, crystallographic analysis, and all atom MD simulations, we investigate mechanisms underlying the binding by NR3A and NR3B of glycine and D-serine, which are candidate neurotransmitters for NMDA receptors containing NR3 subunits. The ligand binding domains of both NR3 subunits adopt a similar extent of domain closure as found in the corresponding NR1 complexes, but have a unique loop 1 structure distinct from that in all other glutamate receptor ion channels. Within their ligand binding pockets, NR3A and NR3B have strikingly different hydrogen bonding networks and solvent structures from those found in NR1, and fail to undergo a conformational rearrangement observed in NR1 upon binding the partial agonist ACPC. MD simulations revealed numerous interdomain contacts, which stabilize the agonist-bound closed-cleft conformation, and a novel twisting motion for the loop 1 helix that is unique in NR3 subunits.

  4. Fast biodegradation of toxic bisphenol a by Pseudomonas aeruginosa NR.22 (Ps.NR.22) isolated from Malaysian local lake

    Science.gov (United States)

    Him, Nik Raikhan Nik; Zainuddin, Mohammad Fiqri; Basha, Anuar Zain Anuar

    2017-12-01

    The paper focused on microbial degradation of Bisphenol A (BPA) as a safe and fast method to reduce BPA contamination in water. BPA is found in waste water, sea water and home water pipeline and it is nondegradable pollutant. Biodegradation is suggested to be practical solution for large volume of BPA. Biodegradation plays an important role and the effect of low concentration significantly decreased the degradation rate. Pseudomonas aeruginosa NR.22 (Ps.NR.22) which has been isolated from a lake at Seksyen 2, Shah Alam, was used. In Malaysia, Ps.NR.22 isolation agar is used for the BPA degradation process. It was stained with Gram negative-rod shaped bacteria that confirmed to carry a 16S rRNA gene. BPA as a sole carbon has been tested at various concentrations. The research showed that BPA was degraded at 10, 20, 30, 40 and 50 ppm and the bacteria growth rate was excellent in 20 ppm BPA. Degradation of BPA was carried out for 9 hours to 18 hours and the maximum degradation was recorded at 9 hours. The value of the highest peak of growth at 540 nm was 2.0617 using 20 ppm BPA. This novel Pseudomonas aeruginosa NR.22 has great potential to be used in waste water treatment.

  5. A new Pseudoscorpion from Bosnia: Roncus bosniensis n. sp. (Neobisiidae, Pseudoscorpiones

    Directory of Open Access Journals (Sweden)

    Ćurčić B.P.M.

    2014-01-01

    Full Text Available A new species of troglobitic false scorpion, Roncus bosniensis n. sp. (Neobisiidae, Pseudoscorpiones, is described from inside an underground habitat, the Jama Pored Puta Pit, nr. Jajce, Bosnia and Herzegovina. The analyzed pseudoscorpion proves to differ from all other congeners, but is closest to R. hajnehaj Ćurčić & Dimitrijević. It is possible that the subterranean pseudoscorpion analyzed represents a relict of an old tropical faunal pattern of the Mediterranean. [Projekat Ministarstva nauke Republike Srbije, br. 173038

  6. Nortriterpene glycosides of the sarasinoside class from the sponge Lipastrotethya sp.

    Science.gov (United States)

    Lee, Jung-Ho; Jeon, Ju-eun; Lee, Yeon-Ju; Lee, Hyi-Seung; Sim, Chung J; Oh, Ki-Bong; Shin, Jongheon

    2012-07-27

    Five new nortriterpene glycosides, along with eight known compounds of the sarasinoside class, were isolated from the tropical sponge Lipastrotethya sp. collected from Chuuk, Micronesia. The structures of these new compounds, designated as sarasinosides N-R (9-13), were determined by combined spectroscopic and chemical methods. The aglycone portions of 10-13 were found to be unprecedented among nortriterpeneoids on the basis of extensive NMR analyses. Several of these compounds exhibited cytotoxicity against A549 and K562 cell lines as well as weak inhibitory activity against Na(+)/K(+)-ATPase.

  7. Cryptosporidium sp. in lizards

    Czech Academy of Sciences Publication Activity Database

    Koudela, Břetislav; Modrý, D.

    1998-01-01

    Roč. 45, č. 1 (1998), s. 8 ISSN 1066-5234. [ Cryptosporidium sp. in lazards. 01.01.1998-02.01.1998, Praha] R&D Projects: GA ČR GA508/95/0273; GA AV ČR IPP2020702 Subject RIV: fp - Other Medical Disciplines

  8. NR2A- and NR2B-Containing NMDA Receptors in the Prelimbic Medial Prefrontal Cortex Differentially Mediate Trace, Delay, and Contextual Fear Conditioning

    Science.gov (United States)

    Gilmartin, Marieke R.; Kwapis, Janine L.; Helmstetter, Fred J.

    2013-01-01

    Activation of "N"-methyl-D-aspartate receptors (NMDAR) in the prelimbic medial prefrontal cortex (PL mPFC) is necessary for the acquisition of both trace and contextual fear memories, but it is not known how specific NR2 subunits support each association. The NR2B subunit confers unique properties to the NMDAR and may differentially…

  9. Roussel. Sinfonien Nr. 3 g-Moll op. 42 und Nr. 4 A-Dur op. 5, Neeme Järvi / Christoph Schlüren

    Index Scriptorium Estoniae

    Schlüren, Christoph

    1995-01-01

    Uuest heliplaadist "Roussel. Sinfonien Nr. 3 g-Moll op. 42 und Nr. 4 A-Dur op. 53, Bacchus et Ariane op. 43 (Zweite Orchestersuite). Sinfonietta für Streichorchester d-Moll op. 52. Detroit Symphony Orchester, N. Järvi". Chandos/Koch CD 7007(WD: 70'10") DDD

  10. The NR-6: A new brief measure of nature relatedness

    Directory of Open Access Journals (Sweden)

    Elizabeth K. Nisbet

    2013-11-01

    Full Text Available The construct of (disconnection with nature or 'nature relatedness' has become increasingly useful in the study of environmental behaviour as well as psychological health and well-being. Strong nature relatedness is associated with greater happiness and ecologically sustainable behaviour. A number of scales reliably assess individual differences in nature relatedness, but some circumstances may necessitate a brief measure. We developed a short-form version of the nature relatedness scale (NR-6, comprised of 6 items from the 'self' and 'experience' dimensions, and tested the new scale's predictive ability across multiple samples and with longitudinal data in students, community members, and business people. The new NR-6 scale demonstrated good internal consistency, temporal stability, and predicted happiness, environmental concern, and nature contact. This new brief measure of connectedness may have advantages where time and space are limited and the research context requires an assessment of connectedness elements rather than environmental attitudes.

  11. NR4A2 is regulated by gastrin and influences cellular responses of gastric adenocarcinoma cells.

    Directory of Open Access Journals (Sweden)

    Kristine Misund

    Full Text Available The peptide hormone gastrin is known to play a role in differentiation, growth and apoptosis of cells in the gastric mucosa. In this study we demonstrate that gastrin induces Nuclear Receptor 4A2 (NR4A2 expression in the adenocarcinoma cell lines AR42J and AGS-GR, which both possess the gastrin/CCK2 receptor. In vivo, NR4A2 is strongly expressed in the gastrin responsive neuroendocrine ECL cells in normal mucosa, whereas gastric adenocarcinoma tissue reveals a more diffuse and variable expression in tumor cells. We show that NR4A2 is a primary early transient gastrin induced gene in adenocarcinoma cell lines, and that NR4A2 expression is negatively regulated by inducible cAMP early repressor (ICER and zinc finger protein 36, C3H1 type-like 1 (Zfp36l1, suggesting that these gastrin regulated proteins exert a negative feedback control of NR4A2 activated responses. FRAP analyses indicate that gastrin also modifies the nucleus-cytosol shuttling of NR4A2, with more NR4A2 localized to cytoplasm upon gastrin treatment. Knock-down experiments with siRNA targeting NR4A2 increase migration of gastrin treated adenocarcinoma AGS-GR cells, while ectopically expressed NR4A2 increases apoptosis and hampers gastrin induced invasion, indicating a tumor suppressor function of NR4A2. Collectively, our results uncover a role of NR4A2 in gastric adenocarcinoma cells, and suggest that both the level and the localization of NR4A2 protein are of importance regarding the cellular responses of these cells.

  12. Tsukamurella inchonensis sp. nov.

    Science.gov (United States)

    Yassin, A F; Rainey, F A; Brzezinka, H; Burghardt, J; Lee, H J; Schaal, K P

    1995-07-01

    Chemotaxonomic and genomic 16S ribosomal DNA sequence analyses of two isolates obtained from two different clinical materials clearly delineated a new species of the genus Tsukamurella. This new species can be identified by its 16S ribosomal DNA similarity values, as well as its physiological characteristics. The name Tsukamurella inchonensis sp. nov. is proposed for these isolates, which are represented by strain IMMIB D-771T (= DSM 44067T) (T = type strain). This strain exhibits only 45% DNA relatedness to Tsukamurella paurometabola.

  13. SP-100 surety evaluation

    International Nuclear Information System (INIS)

    1985-06-01

    This report describes surety evaluations conducted during GFY 1985 in support of the General Electric design for a Space Nuclear Power System - SP-100. Those surety evaluations address both safety and safeguards requirements, which are derived from OSNP-1 and supporting documents. The report includes results of neutronics (criticality) calculations performed by Los Alamos. The results have been benchmarked against independent calculations performed by General Electric with different codes. These comparisons show close agreement, and are summarized. Los Alamos has also provided specifications of explosion and fire environments, which have been used in evaluation of the GE SP-100 concept. Following the summary of key results, surety requirements are given and recommendations toward specification of requirements for later SP-100 project phases are presented. A conceptual design summary is presented. To establish a comprehensive background for surety evaluations, a reference mission profile and potential accidents for each phase of the mission are identified. The main body of the report addresses surety of the General Electric Thermoelectric Conversion design. GE has also developed a Stirling Engine concept, and performed comprehensive surety evaluations for it. These evaluations are reported

  14. On the role of NR3A in human NMDA receptors

    DEFF Research Database (Denmark)

    Eriksson, Maria; Nilsson, Anna; Samuelsson, Helena

    2007-01-01

    In the present paper we describe our on-going project investigating the functional roles of the N-methyl-D-aspartate (NMDA) receptor subunit NR3A. We find that NR3A mRNA is abundant both in embryonic and adult human brain, in contrast to the almost non-existing expression in adult rodent brain...... binding site, with similar affinity as the glycine binding site of NR1 subunits. This suggests that NR3A may replace one of the two NR1 subunits in native NMDA receptors. Cloning of hNR3A showed a human-specific polyproline-sequence in the intracellular C-terminus, that may bind to SH3-domains. We...... interaction assays, binding of PSD-95 to hNR3A could no be demonstrated either in vitro or in vivo. To identify intracellular signaling pathways for NR3A-containing NMDA receptors, we screened for proteins interacting with hNR3A and identified three proteins: plectin, CARP-1 and GPS2. The possible...

  15. Blood compatibility assessment of graft copolymer (NR-g-DMAA) tubes

    International Nuclear Information System (INIS)

    Razzak, M.T.; Otsuhata, Kazushige; Tabata, Yoneho; Ohashi, Fumio; Takeuchi, Atsuki

    1992-01-01

    Graft copolymer (NR-g-DMAA) tubes have been prepared using simultaneous radiation induced grafting of N,N-dimethyl-acrylamide, (DMAA) onto natural rubber (NR) tubes. The blood compatibility of the NR-g-DMAA tubes was assessed with three methods, namely in vitro test, ex vivo once through test and ex vivo loops test. In the case of the in vitro test, a simple whole blood contacting procedure has been employed. The ex vivo once through test involves the exposing of NR-g-DMAA tubes with once through flow of fresh canine blood and then it was inspected for any evidence of clot. In the case of ex vivo loops test, the NR-g-DMAA tube was implanted at external jugular vein of a mongrel canine and the blood flow in the NR-g-DMAA tube was detected with an ultrasonic flow meter. It was found that the blood compatibility of NR-g-DMAA tubes is improved significantly with the increasing degree of grafting. All the NR-g-DMAA tubes having a degree of grafting of about 30 wt% or more exhibit good blood compatibility. It was found that the blood compatibility of the NR-g-DMAA tube is better than that of a medical grade silicon rubber (SiR) tube. (Author)

  16. Reduced levels of NR1 and NR2A with depression-like behavior in different brain regions in prenatally stressed juvenile offspring.

    Directory of Open Access Journals (Sweden)

    Hongli Sun

    Full Text Available Adolescence is a time of continued brain maturation, particularly in limbic and cortical regions, which undoubtedly plays a role in the physiological and emotional changes. Juvenile rats repeatedly exposed to prenatal stress (PS exhibit behavioral features often observed in neuropsychiatric disorders including depression. However, to date the underlying neurological mechanisms are still unclear. In the current study, juvenile offspring rats whose mothers were exposed to PS were evaluated for depression-related behaviors in open field and sucrose preference test. NMDA receptor subunits NR1 and NR2A in the hippocampus, frontal cortex and striatum were assayed by western blotting. The results indicated that PS resulted in several behavioral anomalies in the OFT and sucrose preference test. Moreover, reduced levels of NMDA receptor subunits NR1 and NR2A in the hippocampus, and NR1 in prefrontal cortex and striatum of prenatally stressed juvenile offspring were found. Treatment with MK-801 to pregnant dams could prevent all those changes in the juvenile offspring. Collectivity, these data support the argument that PS to pregnant dams could induce depression-like behavior, which may be involved with abnormal expression of NR1 and NR2A in specific brain regions, and MK-801 may have antidepressant-like effects on the juvenile offspring.

  17. Control of Branchionus sp. and Amoeba sp. in cultures of Arthrospira sp. Control de Branchionus sp. y Amoeba sp. en cultivos de Arthrospira sp.

    Directory of Open Access Journals (Sweden)

    Carlos Méndez

    2012-09-01

    Full Text Available Cultivation of cyanobacterium Arthrospira sp. has been developed in many countries for the production of proteins, pigments and other compounds. Outdoor mass cultures are often affected by biological contamination, drastically reducing productivity as far as bringing death. This study evaluates the control of Branchionus sp. and Amoeba sp. with two chemical compounds: urea (U and ammonium bicarbonate (AB, in laboratory conditions and outdoor mass culture of Arthrospira sp. The lethal concentration 100 (LC100 at 24 h for Branchionus sp. and Amoeba sp. determined was of 60-80 mg L-1 (U and 100-150 mg L-1 (AB. The average effective inhibition concentration for 50% of the population (IC50 in Arthrospira sp., after 72 h, was 80 mg L-1 (U and 150 mg L-1 (AB. The application of doses of 60 mg L-1 (U or 100 mg L-1 (AB in the outdoor mass culture of this contaminated microalga, completely inhibited grazing and did not affect the growth of Arthrospira sp. but rather promoted rapid recovery of algal density at levels prior to infestation. These compounds provided an economical and effective control of predators in cultures of Arthrospira sp.El cultivo de la cianobacteria Arthrospira sp. ha sido desarrollado en muchos países para la obtención de proteínas, pigmentos y otros compuestos. Cultivo que a nivel industrial se ve afectado frecuentemente por contaminación biológica, reduciendo drásticamente la productividad hasta causar la muerte. Este estudio evalúa el control de Branchionus sp. y de Amoeba sp. con dos compuestos químicos, la urea (U y bicarbonato de amonio (AB en cultivos de Arthrospira sp. La concentración letal 100 (LC100 determinada a las 24 h para Branchionus sp. y Amoeba sp. fue de 60-80 mg L-1 (U y 100-150 mg L-1 (AB. La concentración media de inhibición efectiva, después de 72 h, para el 50% de la población (IC50 en Arthrospira fue de 80 mg L-1 (U y 150 mg L-1 (AB. La aplicación de dosis de 60 mg L-1 (U ó 100 mg L-1 (AB en

  18. Ovlivňují anti-NR2A/anti-NR2B oligodeoxynukleotidy hipokampální expresi proteinu NR1 podjednotky NMDA receptoru u dospělého potkana - pilotní studie.

    Czech Academy of Sciences Publication Activity Database

    Vrajová, M.; Tejkalová, H.; Klaschka, Jan; Šťastný, F.

    2008-01-01

    Roč. 12, Suppl. 3 (2008), s. 81-83 ISSN 1212-6845 Grant - others:GA MŠk(CZ) 1M0517 Program:1M Institutional research plan: CEZ:AV0Z10300504 Keywords : animální model schizofrenie * hypofunkce NMDA receptor u * oligodeoxynukleotidy pro NMDA -NR1/NR2 podjednotky * prepulzní inhibice akustického úleku Subject RIV: FL - Psychiatry, Sexuology

  19. Amygdala Infusions of an NR2B-Selective or an NR2A-Preferring NMDA Receptor Antagonist Differentially Influence Fear Conditioning and Expression in the Fear-Potentiated Startle Test

    Science.gov (United States)

    Walker, David L.; Davis, Michael

    2008-01-01

    Within the amygdala, most N-methyl-D-aspartic acid (NMDA) receptors consist of NR1 subunits in combination with either NR2A or NR2B subunits. Because the particular subunit composition greatly influences the receptors' properties, we investigated the contribution of both subtypes to fear conditioning and expression. To do so, we infused the…

  20. Tsukamurella pulmonis sp. nov.

    Science.gov (United States)

    Yassin, A F; Rainey, F A; Brzezinka, H; Burghardt, J; Rifai, M; Seifert, P; Feldmann, K; Schaal, K P

    1996-04-01

    Chemotaxonomic and 16S ribosomal DNA sequence analyses of an isolate from the sputum of a patient with a mycobacterial lung infection clearly delineated a new species of the genus Tsukamurella. This new species can be defined on the basis of genotypic and phenotypic data. The name Tsukamurella pulmonis sp. nov. is proposed for this organism; the type strain is IMMIB D-1321T (= DSM 44142T). This isolate shows 44.2 and 36.2% DNA relatedness to Tsukamurella paurometabola DSM 20162T (T = type strain) and Tsukamurella inchonensis DSM 44067T, respectively.

  1. Absence of NR2E1 mutations in patients with aniridia

    DEFF Research Database (Denmark)

    Corso-Díaz, Ximena; Borrie, Adrienne E; Bonaguro, Russell

    2012-01-01

    Nuclear receptor 2E1 (NR2E1) is a transcription factor with many roles during eye development and thus may be responsible for the occurrence of certain congenital eye disorders in humans. To test this hypothesis, we screened NR2E1 for candidate mutations in patients with aniridia and other...

  2. Host range expansion and increased damage potential of Euwallacea nr. fornicatus (Coleoptera: Curculionidae) in Florida

    Science.gov (United States)

    Ambrosia beetles in the Euwallacea nr. fornicatus complex (Coleoptera: Curculionidae) vector Fusarium spp. fungi pathogenic to susceptible hosts, including avocado. The Florida avocado production area in Miami-Dade County was surveyed for E. nr. fornicatus upon observations of initial damage in 2016...

  3. Tubin: Sinfonie nr. 11 (ergänzt von Kaljo Raid) / Christoph Schlüren

    Index Scriptorium Estoniae

    Schlüren, Christoph

    1997-01-01

    Uuest heliplaadist "Tubin: Sinfonie nr. 11 (ergänzt von Kaljo Raid); Pärt: Nekrolog op. 5, Sinfonie nr. 1; Tüür: Searching for Roots, Insula deserta, Zeitraum. Königliches Philharmonisches Orchester Stockholm, Paavo Järvi". Virgin/EMI CD 5 45212 2 (WD:71'34") DDD

  4. Impaired Discrimination Learning in Mice Lacking the NMDA Receptor NR2A Subunit

    Science.gov (United States)

    Brigman, Jonathan L.; Feyder, Michael; Saksida, Lisa M.; Bussey, Timothy J.; Mishina, Masayoshi; Holmes, Andrew

    2008-01-01

    N-Methyl-D-aspartate receptors (NMDARs) mediate certain forms of synaptic plasticity and learning. We used a touchscreen system to assess NR2A subunit knockout mice (KO) for (1) pairwise visual discrimination and reversal learning and (2) acquisition and extinction of an instrumental response requiring no pairwise discrimination. NR2A KO mice…

  5. Characterisation of the human NMDA receptor subunit NR3A glycine binding site

    DEFF Research Database (Denmark)

    Nilsson, A; Duan, J; Mo-Boquist, L-L

    2007-01-01

    In this study, we characterise the binding site of the human N-methyl-d-aspartate (NMDA) receptor subunit NR3A. Saturation radioligand binding of the NMDA receptor agonists [(3)H]-glycine and [(3)H]-glutamate showed that only glycine binds to human NR3A (hNR3A) with high affinity (K(d)=535nM (277......-793nM)). Eight amino acids, which correspond to amino acids that are critical for ligand binding to other NMDA receptor subunits, situated within the S1S2 predicted ligand binding domain of hNR3A were mutated, which resulted in complete or near complete loss of [(3)H]-glycine binding to hNR3A. The NMDA...

  6. Molecular pathways: the role of NR4A orphan nuclear receptors in cancer.

    LENUS (Irish Health Repository)

    Mohan, Helen M

    2012-06-15

    Nuclear receptors are of integral importance in carcinogenesis. Manipulation of classic ligand-activated nuclear receptors, such as estrogen receptor blockade in breast cancer, is an important established cancer therapy. Orphan nuclear receptors, such as nuclear family 4 subgroup A (NR4A) receptors, have no known natural ligand(s). These elusive receptors are increasingly recognized as molecular switches in cell survival and a molecular link between inflammation and cancer. NR4A receptors act as transcription factors, altering expression of downstream genes in apoptosis (Fas-ligand, TRAIL), proliferation, DNA repair, metabolism, cell migration, inflammation (interleukin-8), and angiogenesis (VEGF). NR4A receptors are modulated by multiple cell-signaling pathways, including protein kinase A\\/CREB, NF-κB, phosphoinositide 3-kinase\\/AKT, c-jun-NH(2)-kinase, Wnt, and mitogen-activated protein kinase pathways. NR4A receptor effects are context and tissue specific, influenced by their levels of expression, posttranslational modification, and interaction with other transcription factors (RXR, PPAR-Υ). The subcellular location of NR4A "nuclear receptors" is also important functionally; novel roles have been described in the cytoplasm where NR4A proteins act both indirectly and directly on the mitochondria to promote apoptosis via Bcl-2. NR4A receptors are implicated in a wide variety of malignancies, including breast, lung, colon, bladder, and prostate cancer; glioblastoma multiforme; sarcoma; and acute and\\/or chronic myeloid leukemia. NR4A receptors modulate response to conventional chemotherapy and represent an exciting frontier for chemotherapeutic intervention, as novel agents targeting NR4A receptors have now been developed. This review provides a concise clinical overview of current knowledge of NR4A signaling in cancer and the potential for therapeutic manipulation.

  7. Acetobacter intermedius, sp. nov.

    Science.gov (United States)

    Boesch, C; Trcek, J; Sievers, M; Teuber, M

    1998-03-01

    Strains of a new species in the genus Acetobacter, for which we propose the name A. intermedius sp. nov., were isolated and characterized in pure culture from different sources (Kombucha beverage, cider vinegar, spirit vinegar) and different countries (Switzerland, Slovenia). The isolated strains grow in media with 3% acetic acid and 3% ethanol as does A. europaeus, do, however, not require acetic acid for growth. These characteristics phenotypically position A. intermedius between A. europaeus and A. xylinus, DNA-DNA hybridizations of A. intermedius-DNA with DNA of the type strains of Acetobacter europaeus, A. xylinus, A. aceti, A. hansenii, A. liquefaciens, A. methanolicus, A. pasteurianus, A. diazotrophicus, Gluconobacter oxydans and Escherichia coli HB 101 indicated less than 60% DNA similarity. The important features of the new species are described. Acetobacter intermedius strain TF2 (DSM11804) isolated from the liquid phase of a tea fungus beverage (Kombucha) is the type strain.

  8. Tsukamurella tyrosinosolvens sp. nov.

    Science.gov (United States)

    Yassin, A F; Rainey, F A; Burghardt, J; Brzezinka, H; Schmitt, S; Seifert, P; Zimmermann, O; Mauch, H; Gierth, D; Lux, I; Schaal, K P

    1997-07-01

    Chemotaxonomic and 16S ribosomal DNA sequence analyses of four bacterial isolates from blood cultures from patients with cardiac pacemaker implants and sputa of patients with chronic lung infections clearly demonstrated that these bacteria belong to the genus Tsukamurella. DNA-DNA hybridization data, as well as the physiological characteristics of the isolates, indicate that they are closely related and belong to a single species that differs from previously described members of the genus Tsukamurella. The name Tsukamurella tyrosinosolvens sp. nov. is proposed for these isolates, and the new species is represented by strain IMMIB D-1397T (= DSM 44234T). Strain IMMIB D-1397T exhibits 53.4, 53.5, and 54.7% DNA-DNA relatedness to Tsukamurella paurometabola DSM 20162T, Tsukamurella inchonensis DSM 44067T, and Tsukamurella pulmonis DSM 44142T, respectively.

  9. The diversity of Terfezia desert truffles: new species and a highly variable species complex with intrasporocarpic nrDNA ITS heterogeneity.

    Science.gov (United States)

    Kovács, Gábor M; Balázs, Tímea K; Calonge, Francisco D; Martín, María P

    2011-01-01

    Desert truffles belonging to Terfezia are well known mycorrhizal members of the mycota of the Mediterranean region and the Middle East. We aimed to test (i) whether the morphological criteria of Terfezia species regularly collected in Spain enable their separation and (ii) whether the previously hypothesized edaphic/biotic specificity of one group could be confirmed by study of a larger number of specimens. The species T. arenaria and T. claveryi can be identified unambiguously by morphological characters. We consider T. leptoderma as a distinct species while several lineages of similar spiny spored Terfezia truffles with cellular peridium were detected that have no obvious anatomical differences. Several species treated generally as synonyms of T. olbiensis have been described in this group, and because they cannot be unambiguously assigned to separate lineages we propose to consider the group as the T. olbiensis species complex. A high level of intrasporocarpic variation of the nrDNA ITS was detected in the T. olbiensis species complex, especially in one of its lineages. We detected no exclusive specificity to either plant associates or soil, except in T. leptoderma, which was associated with Quercus spp. and cistaceous plants on acidic soils. Nevertheless the clades showed a tendency either to associate with Quercus/Helianthemum/Cistus or Pinus hosts. Specimens having distinct anatomical features, reticulate spores and cellular peridium formed a separate group in the molecular phylogenetic analyses of nrDNA ITS and LSU regions; for these specimens we propose a new species, Terfezia alsheikhii sp. nov.

  10. Interaction of ApoA-IV with NR4A1 and NR1D1 Represses G6Pase and PEPCK Transcription: Nuclear Receptor-Mediated Downregulation of Hepatic Gluconeogenesis in Mice and a Human Hepatocyte Cell Line.

    Science.gov (United States)

    Li, Xiaoming; Xu, Min; Wang, Fei; Ji, Yong; DavidsoN, W Sean; Li, Zongfang; Tso, Patrick

    2015-01-01

    We have previously shown that the nuclear receptor, NR1D1, is a cofactor in ApoA-IV-mediated downregulation of gluconeogenesis. Nuclear receptor, NR4A1, is involved in the transcriptional regulation of various genes involved in inflammation, apoptosis, and glucose metabolism. We investigated whether NR4A1 influences the effect of ApoA-IV on hepatic glucose metabolism. Our in situ proximity ligation assays and coimmunoprecipitation experiments indicated that ApoA-IV colocalized with NR4A1 in human liver (HepG2) and kidney (HEK-293) cell lines. The chromatin immunoprecipitation experiments and luciferase reporter assays indicated that the ApoA-IV and NR4A1 colocalized at the RORα response element of the human G6Pase promoter, reducing its transcriptional activity. Our RNA interference experiments showed that knocking down the expression of NR4A1 in primary mouse hepatocytes treated with ApoA-IV increased the expression of NR1D1, G6Pase, and PEPCK, and that knocking down NR1D1 expression increased the level of NR4A1. We also found that ApoA-IV induced the expression of endogenous NR4A1 in both cultured primary mouse hepatocytes and in the mouse liver, and decreased glucose production in primary mouse hepatocytes. Our findings showed that ApoA-IV colocalizes with NR4A1, which suppresses G6Pase and PEPCK gene expression at the transcriptional level, reducing hepatic glucose output and lowering blood glucose. The ApoA-IV-induced increase in NR4A1 expression in hepatocytes mediates further repression of gluconeogenesis. Our findings suggest that NR1D1 and NR4A1 serve similar or complementary functions in the ApoA-IV-mediated regulation of gluconeogenesis.

  11. Blockade of NR2A-Containing NMDA Receptors Induces Tau Phosphorylation in Rat Hippocampal Slices

    Directory of Open Access Journals (Sweden)

    Julie Allyson

    2010-01-01

    Full Text Available Physiological activation of the N-methyl-D-aspartate (NMDA subtype of glutamate receptors has been proposed to play a key role in both neuronal cell function and dysfunction. In the present study, we used selective NMDA receptor antagonists to investigate the involvement of NR2A and NR2B subunits in the modulatory effect of basal NMDA receptor activity on the phosphorylation of Tau proteins. We observed, in acute hippocampal slice preparations, that blockade of NR2A-containing NMDA receptors by the NR2A antagonist NVP-AAM077 provoked the hyperphosphorylation of a residue located in the proline-rich domain of Tau (i.e., Ser199. This effect seemed to be Ser199 specific as there was no increase in phosphorylation at Ser262 and Ser409 residues located in the microtubule-binding and C-terminal domains of Tau proteins, respectively. From a mechanistic perspective, our study revealed that blockade of NR2A-containing receptors influences Tau phosphorylation probably by increasing calcium influx into neurons, which seems to rely on accumulation of new NR1/NR2B receptors in neuronal membranes and could involve the cyclin-dependent kinase 5 pathway.

  12. Study of rheological, viscoelastic and vulcanization behavior of sponge EPDM/NR blended nano- composites

    International Nuclear Information System (INIS)

    Bashir, M Arshad; Shahid, M; Ahmed, Riaz; Yahya, A G

    2014-01-01

    In this research paper the effect of blending ratio of natural rubber (NR) with Ethylene Propylene Diene Monomer (EPDM) were investigated. Different samples of EPDM/NR ratio were prepared to study the variation of NR in EPDM on rheology, curing characteristics, tangent δ, and viscosity variation during vulcanization of sponge nano composites.The main aim of present research is to develop elastomeric based sponge composites with the blending ratio of base elastomers along with the carbon nano particles for high energy absorbing and damping applications. The curing characteristics, rheology and viscoelastic nature of the composite is remarkably influenced with the progressive blending ratio of the base elastomeric matrix

  13. Study of rheological, viscoelastic and vulcanization behavior of sponge EPDM/NR blended nano-composites

    International Nuclear Information System (INIS)

    Bashir, M. A.; Shahid, M.; Ahmed, R.; Yahya, A. G.

    2013-01-01

    In this research paper the effect of blending ratio of natural rubber (NR) with Ethylene Propylene Diene Monomer (EPDM) were investigated. Different samples of EPDM/NR ratio were prepared to study the variation of NR in EPDM on rheology, curing characteristics, tangent d, and viscosity variation during vulcanization of sponge nano composites. The main aim of present research is to develop elastomeric based sponge composites with the blending ratio of base elastomers along with the carbon nano particles for high energy absorbing and damping applications. The curing characteristics, rheology and viscoelastic nature of the composite is remarkably influenced with the progressive blending ratio of the base elastomeric matrix. (author)

  14. Bradysia sp. em morangueiro Bradysia sp. in strawberry

    Directory of Open Access Journals (Sweden)

    Bernadete Radin

    2009-04-01

    Full Text Available No trabalho, relatam-se os primeiros registros de Bradysia sp. (Insecta: Diptera: Sciaridae em morangueiro (Fragaria x ananassa Duch., cultivado no Município de Eldorado do Sul, RS. O cultivo foi realizado em sacolas com três metros de comprimento, preenchidas com substrato composto de casca de arroz e turfa, dispostas horizontalmente sobre bancadas de madeira, em ambiente protegido. A presença de Bradysia sp. foi observada na segunda quinzena de agosto de 2005. Neste trabalho, estão descritos os sintomas apresentados no morangueiro pela praga, prováveis conseqüências sobre o aparecimento de doenças e uma breve descrição morfológica da Bradysia sp., adulto e fase larval.This paper describes the first record of Bradysia sp. (Insecta; Diptera; Sciaridae in strawberry (Fragaria x ananassa, cultivated in the city of Eldorado do Sul, RS, Brazil. Strawberry was planted in plastic bags filled with a mixture of burnt rice hulls and peat and cultivated in a greenhouse. The presence of Bradysia sp was noticed in the second fortnight of August, 2005. The symptoms in strawberry and the probable consequences in terms of disease arising were described in the present study, as well as the morphological characterization of Bradysia sp. and its illustrations.

  15. Keno-Nr a Monte Carlo Code Simulating the Californium -252-SOURCE-DRIVEN Noise Analysis Experimental Method for Determining Subcriticality

    Science.gov (United States)

    Ficaro, Edward Patrick

    The ^{252}Cf -source-driven noise analysis (CSDNA) requires the measurement of the cross power spectral density (CPSD) G_ {23}(omega), between a pair of neutron detectors (subscripts 2 and 3) located in or near the fissile assembly, and the CPSDs, G_{12}( omega) and G_{13}( omega), between the neutron detectors and an ionization chamber 1 containing ^{252}Cf also located in or near the fissile assembly. The key advantage of this method is that the subcriticality of the assembly can be obtained from the ratio of spectral densities,{G _sp{12}{*}(omega)G_ {13}(omega)over G_{11 }(omega)G_{23}(omega) },using a point kinetic model formulation which is independent of the detector's properties and a reference measurement. The multigroup, Monte Carlo code, KENO-NR, was developed to eliminate the dependence of the measurement on the point kinetic formulation. This code utilizes time dependent, analog neutron tracking to simulate the experimental method, in addition to the underlying nuclear physics, as closely as possible. From a direct comparison of simulated and measured data, the calculational model and cross sections are validated for the calculation, and KENO-NR can then be rerun to provide a distributed source k_ {eff} calculation. Depending on the fissile assembly, a few hours to a couple of days of computation time are needed for a typical simulation executed on a desktop workstation. In this work, KENO-NR demonstrated the ability to accurately estimate the measured ratio of spectral densities from experiments using capture detectors performed on uranium metal cylinders, a cylindrical tank filled with aqueous uranyl nitrate, and arrays of safe storage bottles filled with uranyl nitrate. Good agreement was also seen between simulated and measured values of the prompt neutron decay constant from the fitted CPSDs. Poor agreement was seen between simulated and measured results using composite ^6Li-glass-plastic scintillators at large subcriticalities for the tank of

  16. SP-100 reactor design

    International Nuclear Information System (INIS)

    Armijo, J.S.; Atwell, J.; Pluta, P.R.; Smith, M.A.; Solorzano, E.R.

    1987-01-01

    The SP-100 space reactor power system is being designed and developed as part of the Ground Engineering System (GES) contract between General Electric Company as the system developer and the Department of Energy. Other key participants in the GES program include Westinghouse Hanford Company (site operator), Los Alamos National Laboratory (fuel development and production), Oak Ridge National Laboratory (materials), and Westinghouse, Advanced Energy Systems Division (shield, HTS equipment). The GES Program includes two major elements. First, the development of a Reference Flight System design at 100 kWe output to the user, and second the validation of the Reference Flight System design by analysis and by testing. Development of key technologies along with component and system testing is an essential part of the validation program. The nuclear subsystem validation includes the design, manufacture, assembly and operational testing of a Ground Reactor Test Assembly. The subject of this paper is the reactor design for the Reference Flight System. The reference flight design is in the preliminary design stage and will evolve over the next year

  17. Postsynaptic density protein 95-regulated NR2B tyrosine phosphorylation and interactions of Fyn with NR2B in levodopa-induced dyskinesia rat models

    Directory of Open Access Journals (Sweden)

    Ba M

    2014-12-01

    Full Text Available Maowen Ba,1,* Min Kong,2,* Guozhao Ma3 1Department of Neurology, Yuhuangding Hospital, Yantai City, Shandong, People’s Republic of China; 2Department of Neurology, Yantaishan Hospital, Yantai City, Shandong, People’s Republic of China; 3Department of Neurology, Shandong Provincial Qianfoshan Hospital, Shandong University, Shandong, People’s Republic of China *These authors contributed equally to this work Context: Abnormality in interactions between N-methyl-d-aspartate (NMDA receptor and its signaling molecules occurs in the lesioned striatum in Parkinson’s disease (PD and levodopa-induced dyskinesia (LID. It was reported that Fyn-mediated NR2B tyrosine phosphorylation, can enhance NMDA receptor function. Postsynaptic density protein 95 (PSD-95, one of the synapse-associated proteins, regulates interactions between receptor and downstream-signaling molecules. In light of the relationship between PSD-95, NR2B, and Fyn kinases, does PSD-95 contribute to the overactivity of NMDA receptor function induced by dopaminergic treatment? To further prove the possibility, the effects of regulating the PSD-95 expression on the augmented NR2B tyrosine phosphorylation and on the interactions of Fyn and NR2B in LID rat models were evaluated.Methods: In the present study, parkinsonian rat models were established by injecting 6-hydroxydopamine. Subsequently, valid PD rats were treated with levodopa (50 mg/kg/day with benserazide 12.5 mg/kg/day, twice daily intraperitoneally for 22 days to create LID rat models. Then, the effect of pretreatment with an intrastriatal injection of the PSD-95mRNA antisense oligonucleotides (PSD-95 ASO on the rotational response to levodopa challenge was assessed. The effects of pretreatment with an intrastriatal injection of PSD-95 ASO on the augmented NR2B tyrosine phosphorylation and interactions of Fyn with NR2B in the LID rat models were detected by immunoblotting and immunoprecipitation. Results: Levodopa

  18. Elaphroporia ailaoshanensis gen. et sp. nov. in Polyporales (Basidiomycota

    Directory of Open Access Journals (Sweden)

    Zi-Qiang Wu

    2018-01-01

    Full Text Available A new poroid wood-inhabiting fungal genus, Elaphroporia, typified by E. ailaoshanensis sp. nov., is proposed based on a combination of morphological features and molecular evidence. The genus is characterised by an annual growth habit, resupinate basidiocarps, becoming rigid and light-weight up on drying, a monomitic hyphal system with thick-walled generative hyphae bearing both clamp connections and simple septa, slightly amyloid, CB+ and ellipsoid, hyaline, thin-walled, smooth and IKI–, CB– basidiospores. Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analysis based on molecular data of ITS+nLSU sequences showed that Elaphroporia belonged to the residual polyporoid clade and was closely related to Junghuhnia crustacea. Further investigation was obtained for more representative taxa in the Meruliaceae based on ITS+nLSU sequences, in which the result demonstrated that the genus Elaphroporia formed a monophyletic lineage with a strong support (100 % BS, 100 % BP, 1.00 BPP and then grouped with Flaviporus and Steccherinum.

  19. Kalinnikov: Sinfonie Nr.2 A-Dur, Ouvertüre Zar Boris / Hanspeter Krellmann

    Index Scriptorium Estoniae

    Krellmann, Hanspeter

    1990-01-01

    Uuest heliplaadist "Kalinnikov: Sinfonie Nr.2 A-Dur, Ouvertüre Zar Boris, Die Zeder und die Palme. Scottish National Orchestra, Neeme Järvi" (AD: 1989) Chandos / Koch Records CD 8805 (WD: 59'40") DDD

  20. The LANL C-NR counting room and fission product yields

    Energy Technology Data Exchange (ETDEWEB)

    Jackman, Kevin Richard [Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

    2015-09-21

    This PowerPoint presentation focused on the following areas: LANL C-NR counting room; Fission product yields; Los Alamos Neutron wheel experiments; Recent experiments ad NCERC; and Post-detonation nuclear forensics

  1. Cyclic benzamidines as orally efficacious NR2B-selective NMDA receptor antagonists.

    Science.gov (United States)

    Nguyen, Kevin T; Claiborne, Christopher F; McCauley, John A; Libby, Brian E; Claremon, David A; Bednar, Rodney A; Mosser, Scott D; Gaul, Stanley L; Connolly, Thomas M; Condra, Cindra L; Bednar, Bohumil; Stump, Gary L; Lynch, Joseph J; Koblan, Kenneth S; Liverton, Nigel J

    2007-07-15

    A novel series of cyclic benzamidines was synthesized and shown to exhibit NR2B-subtype selective NMDA antagonist activity. Compound 29 is orally active in a carrageenan-induced rat hyperalgesia model of pain.

  2. Wide spectrum of NR5A1-related phenotypes in 46,XY and 46,XX individuals.

    Science.gov (United States)

    Domenice, Sorahia; Machado, Aline Zamboni; Ferreira, Frederico Moraes; Ferraz-de-Souza, Bruno; Lerario, Antonio Marcondes; Lin, Lin; Nishi, Mirian Yumie; Gomes, Nathalia Lisboa; da Silva, Thatiana Evelin; Silva, Rosana Barbosa; Correa, Rafaela Vieira; Montenegro, Luciana Ribeiro; Narciso, Amanda; Costa, Elaine Maria Frade; Achermann, John C; Mendonca, Berenice Bilharinho

    2016-12-01

    Steroidogenic factor 1 (NR5A1, SF-1, Ad4BP) is a transcriptional regulator of genes involved in adrenal and gonadal development and function. Mutations in NR5A1 have been among the most frequently identified genetic causes of gonadal development disorders and are associated with a wide phenotypic spectrum. In 46,XY individuals, NR5A1-related phenotypes may range from disorders of sex development (DSD) to oligo/azoospermia, and in 46,XX individuals, from 46,XX ovotesticular and testicular DSD to primary ovarian insufficiency (POI). The most common 46,XY phenotype is atypical or female external genitalia with clitoromegaly, palpable gonads, and absence of Müllerian derivatives. Notably, an undervirilized external genitalia is frequently seen at birth, while spontaneous virilization may occur later, at puberty. In 46,XX individuals, NR5A1 mutations are a rare genetic cause of POI, manifesting as primary or secondary amenorrhea, infertility, hypoestrogenism, and elevated gonadotropin levels. Mothers and sisters of 46,XY DSD patients carrying heterozygous NR5A1 mutations may develop POI, and therefore require appropriate counseling. Moreover, the recurrent heterozygous p.Arg92Trp NR5A1 mutation is associated with variable degrees of testis development in 46,XX patients. A clear genotype-phenotype correlation is not seen in patients bearing NR5A1 mutations, suggesting that genetic modifiers, such as pathogenic variants in other testis/ovarian-determining genes, may contribute to the phenotypic expression. Here, we review the published literature on NR5A1-related disease, and discuss our findings at a single tertiary center in Brazil, including ten novel NR5A1 mutations identified in 46,XY DSD patients. The ever-expanding phenotypic range associated with NR5A1 variants in XY and XX individuals confirms its pivotal role in reproductive biology, and should alert clinicians to the possibility of NR5A1 defects in a variety of phenotypes presenting with gonadal dysfunction

  3. Dynamic aperture of NR-2000 in the work regime with a small radiation emittance

    International Nuclear Information System (INIS)

    Zelinskij, A.Yu.; Karnaukhov, I.M.; Kononenko, S.G.; Tarasenko, A.S.

    1991-01-01

    NR-2000 is an electron storage ring installation with 6-pole magnets compensating its chromatism. The results of NR-2000 dynamic aperture numerical modelling in 6-dimension space are given. Perturbation of the equilibrium orbit and that of magnetic field up to the third order of magnitude were taken into consideration. Calculations were made according to DeCA programme. 7 refs.; 5 figs. (author)

  4. Exprese NR1 podjednotky NMDA receptoru v animálním modelu schizofrenie

    Czech Academy of Sciences Publication Activity Database

    Šťastný, František; Kozmiková, I.; Klaschka, Jan; Peková, S.; Tejkalová, H.; Vrajová, M.

    2006-01-01

    Roč. 10, Suppl. 3 (2006), s. 12-15 ISSN 1211-7579 R&D Projects: GA MŠk(CZ) 1M0517; GA MZd NF7626; GA MZd NR8797 Institutional research plan: CEZ:AV0Z10300504; CEZ:AV0Z50110509 Keywords : schizofrenie * NMDA receptor * exprese NR1 podjednotky * animální model * kyselina chinolinová * prepulzní inhibice akustického úletu Subject RIV: FL - Psychiatry, Sexuology

  5. Penicillium araracuarense sp. nov., Penicillium elleniae sp. nov., Penicillium penarojense sp. nov., Penicillium vanderhammenii sp. nov. and Penicillium wotroi sp. nov., isolated from leaf litter

    DEFF Research Database (Denmark)

    Houbraken, Jos; López-Quintero, Carlos A.; Frisvad, Jens Christian

    2011-01-01

    Several species of the genus Penicillium were isolated during a survey of the mycobiota of leaf litter and soil in Colombian Amazon forest. Five species, Penicillium penarojense sp. nov. (type strain CBS 113178T = IBT 23262T), Penicillium wotroi sp. nov. (type strain CBS 118171T = IBT 23253T......), Penicillium araracuarense sp. nov. (type strain CBS 113149T = IBT 23247T), Penicillium elleniae sp. nov. (type strain CBS 118135T = IBT 23229T) and Penicillium vanderhammenii sp. nov. (type strain CBS 126216T = IBT 23203T) are described here as novel species. Their taxonomic novelty was determined using......, andrastin A, pulvilloric acid, paxillin, paspaline and janthitrem, were commonly produced by these phylogenetically related species. The novel species had a high growth rate on agar media, but could be distinguished from each other by several macro- and microscopical characteristics....

  6. Uji Fitokimia, Kandungan Total Fenol dan Aktivitas Antioksidan Mikroalga Spirulina sp., Chlorella sp., dan Nannochloropsis sp.

    Directory of Open Access Journals (Sweden)

    Diini Fithriani

    2015-12-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui kandungan fitokimia, total fenol, dan aktivitas antioksidan dari mikroalga Spirulina sp., Nannochloropsis sp., dan Chlorella sp. Mikroalga diekstrak dengan ekstraksi tunggal menggunakan etanol. Skrining fitokimia dilakukan secara kualitatif. Analisis total fenol dilakukan secara spektrofotometri dengan metode Folin-Ciocalteu. Analisis antioksidan dilakukan dengan metode 2,2-diphenyl-1-picrylhydrazyl (DPPH dan Ferric Reducing Antioxidant Power (FRAP. Skrining fitokimia menunjukkan keberadaan tanin, flavonoid, steroid, glikosida, dan alkaloid pada ekstrak etanol ketiga jenis mikroalga, sedangkan saponin hanya terdeteksi pada ekstrak etanol Spirulina sp. dan Chlorella sp., adapun triterpenoid tidak terdeteksi pada ekstrak etanol ketiga jenis mikroalga. Hasil penelitian menunjukkan bahwa kandungan total fenol, aktivitas antioksidan (IC50, dan kapasitas antioksidan (FRAP tertinggi diperoleh pada ekstrak etanol Spirulina sp. dengan nilai berturut turut sebesar 0,32 ± 0,025 mg GAE g-1D.W., 518,94 ppm, dan  49,95 ± 2,02 (mmol Fe2+ eq.g-1D.W. Dalam penelitian ini diketahui bahwa kandungan fenol total memiliki korelasi  yang kuat  dengan kapasitas antioksidan metode FRAP (R2= 0,84, dan aktivitas antioksidan metode DPPH (R2= 0,79.

  7. Uji Fitokimia, Kandungan Total Fenol Dan Aktivitas Antioksidan Mikroalga Spirulina Sp., Chlorella Sp., dan Nannochloropsis Sp.

    Directory of Open Access Journals (Sweden)

    Diini Fithriani

    2015-12-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui kandungan fitokimia, total fenol, dan aktivitas antioksidan dari mikroalga Spirulina sp., Nannochloropsis sp., dan Chlorella sp. Mikroalga diekstrak dengan ekstraksi tunggal menggunakan etanol. Skrining fitokimia dilakukan secara kualitatif. Analisis total fenol dilakukan secara spektrofotometri dengan metode Folin-Ciocalteu. Analisis antioksidan dilakukan dengan metode 2,2-diphenyl-1-picrylhydrazyl (DPPH dan Ferric Reducing Antioxidant Power (FRAP. Skrining fitokimia menunjukkan keberadaan tanin, flavonoid, steroid, glikosida, dan alkaloid pada ekstrak etanol ketiga jenis mikroalga, sedangkan saponin hanya terdeteksi pada ekstrak etanol Spirulina sp. dan Chlorella sp., adapun triterpenoid tidak terdeteksi pada ekstrak etanol ketiga jenis mikroalga. Hasil penelitian menunjukkan bahwa kandungan total fenol, aktivitas antioksidan (IC50, dan kapasitas antioksidan (FRAP tertinggi diperoleh pada ekstrak etanol Spirulina sp. dengan nilai berturut turut sebesar 0,32 ± 0,025 mg GAE g-1D.W., 518,94 ppm, dan  49,95 ± 2,02 (mmol Fe2+ eq.g-1D.W. Dalam penelitian ini diketahui bahwa kandungan fenol total memiliki korelasi  yang kuat  dengan kapasitas antioksidan metode FRAP (R2= 0,84, dan aktivitas antioksidan metode DPPH (R2= 0,79.

  8. iNR-Drug: Predicting the Interaction of Drugs with Nuclear Receptors in Cellular Networking

    Directory of Open Access Journals (Sweden)

    Yue-Nong Fan

    2014-03-01

    Full Text Available Nuclear receptors (NRs are closely associated with various major diseases such as cancer, diabetes, inflammatory disease, and osteoporosis. Therefore, NRs have become a frequent target for drug development. During the process of developing drugs against these diseases by targeting NRs, we are often facing a problem: Given a NR and chemical compound, can we identify whether they are really in interaction with each other in a cell? To address this problem, a predictor called “iNR-Drug” was developed. In the predictor, the drug compound concerned was formulated by a 256-D (dimensional vector derived from its molecular fingerprint, and the NR by a 500-D vector formed by incorporating its sequential evolution information and physicochemical features into the general form of pseudo amino acid composition, and the prediction engine was operated by the SVM (support vector machine algorithm. Compared with the existing prediction methods in this area, iNR-Drug not only can yield a higher success rate, but is also featured by a user-friendly web-server established at http://www.jci-bioinfo.cn/iNR-Drug/, which is particularly useful for most experimental scientists to obtain their desired data in a timely manner. It is anticipated that the iNR-Drug server may become a useful high throughput tool for both basic research and drug development, and that the current approach may be easily extended to study the interactions of drug with other targets as well.

  9. iNR-Drug: predicting the interaction of drugs with nuclear receptors in cellular networking.

    Science.gov (United States)

    Fan, Yue-Nong; Xiao, Xuan; Min, Jian-Liang; Chou, Kuo-Chen

    2014-03-19

    Nuclear receptors (NRs) are closely associated with various major diseases such as cancer, diabetes, inflammatory disease, and osteoporosis. Therefore, NRs have become a frequent target for drug development. During the process of developing drugs against these diseases by targeting NRs, we are often facing a problem: Given a NR and chemical compound, can we identify whether they are really in interaction with each other in a cell? To address this problem, a predictor called "iNR-Drug" was developed. In the predictor, the drug compound concerned was formulated by a 256-D (dimensional) vector derived from its molecular fingerprint, and the NR by a 500-D vector formed by incorporating its sequential evolution information and physicochemical features into the general form of pseudo amino acid composition, and the prediction engine was operated by the SVM (support vector machine) algorithm. Compared with the existing prediction methods in this area, iNR-Drug not only can yield a higher success rate, but is also featured by a user-friendly web-server established at http://www.jci-bioinfo.cn/iNR-Drug/, which is particularly useful for most experimental scientists to obtain their desired data in a timely manner. It is anticipated that the iNR-Drug server may become a useful high throughput tool for both basic research and drug development, and that the current approach may be easily extended to study the interactions of drug with other targets as well.

  10. NMDAR NR2A and NR2B specific PKC-dependent regulation of mGluR is defective in the Fragile X Syndrome mouse model

    DEFF Research Database (Denmark)

    Banke, Tue G.; Toft, Anna Karina; Lundbye, Camilla Johanne

    ). Similar results were observed when mature KO slices were preincubated with the protein kinase C activator (PMA), or inactivator (chelerythrine), suggesting that NMDAR activation leads to PKC regulation of mGluR-LTD. Surprisingly, preincubation WT with the NR2B specific NMDAR inhibitor CP-101.......606 completely blocked DHPG-induced LTD. This blockage was reversed by either preincubation with PMA or by co-application of the NR2A antagonist TCN-201. In contrast, application of TCN-201 alone had no apparent effects. Our data suggest a model where NMDARs regulate mGluR-LTD through regulation of PKC...

  11. In vivo assessment of the hepatotoxicity of a new Nostoc isolate from the Nile River: Nostoc sp. strain NRI.

    Science.gov (United States)

    Abu-Serie, Marwa M; Nasser, Nermine; Abd El-Wahab, Abeer; Shehawy, Rehab; Pienaar, Harrison; Baddour, Nahed; Amer, Ranya

    2018-03-01

    Nostoc sp. is one of the most widely distributed cyanobacterial genera that produce potentially protein phosphatase (PP) inhibitor; microcystins (MCs). MCs have posed a worldwide concern due to predominant hepatotoxicity to human health. We have previously isolated a Nostoc strain (NR1) from the Nile River (the main water supply in Egypt) and this strain exerted production of rare and highly toxic MC; demethylated microcystin-LR. There is no data concerning risk factors of liver diseases for human and animal exposure to NR1-contaminated drinking water yet. It is thus important to evaluate acute (LD 50 dose), subacute (0.01% and 10% of LD 50 dose) and subchronic (0.01% and 10% of LD 50 dose) hepatotoxicity's NR1 extract using experimental mice. Mice groups, who orally received 0.01% LD 50 , represented a permissible concentration of the World Health Organization (WHO) for MC in drinking water. Several parameters were detected, including hepatotoxicity (i.e. PP activity, liver function, oxidative stress markers and DNA fragmentation), pro-inflammatory cytokine (TNF-α) and liver histopathology. Our results demonstrated LD 50 of NR1 extract was at 15,350 mg/kg body weight and caused hepatotoxicity that attributed to PP inhibition and a significant increase of hepatic damage biomarkers with lipid accumulation. Moreover, NR1 extract induced hepatic oxidative damage that may have led to DNA fragmentation and production of TNF-α. As demonstrated from the histopathological study, NR1 extract caused a severe collapse of cytoskeleton with subsequent focal degeneration of hepatocytes, necroinflammation and steatosis. The grade of hepatotoxicity in subacute (10% of LD 50 ) group was higher than that in the subchronic (10% of LD 50 and 0.01% of LD 50 , WHOch, respectively) groups. No significant hepatotoxicity was detectable for subacute (0.01% of LD 50 , WHOac) group. NR1 is therefore considered as one of the harmful and life-threatening cyanobacteria for Egyptian people

  12. NR2F6 Expression Correlates with Pelvic Lymph Node Metastasis and Poor Prognosis in Early-Stage Cervical Cancer

    Directory of Open Access Journals (Sweden)

    Chunhao Niu

    2016-10-01

    Full Text Available Background: There is an abnormal expression of nuclear receptor subfamily 2 group F member 6 (NR2F6 in human cancers such as breast cancer, colon cancer, and acute myelogenous leukemia. However, its clinical significance in cervical cancer has not been established. We explored NR2F6 expression and its clinicopathological significance in early-stage cervical cancer. Methods: NR2F6 expression in cervical cancer cell lines and cervical cancer tissues was determined by Western blotting, real-time PCR, and immunochemistry (IHC. NR2F6 expression in 189 human early-stage cervical cancer tissue samples was evaluated using IHC. The relevance between NR2F6 expression and early-stage cervical cancer prognosis and clinicopathological features was determined. Results: There was marked NR2F6 mRNA and protein overexpression in the cervical cancer cells and clinical tissues compared with an immortalized squamous cell line and adjacent noncancerous cervical tissues, respectively. In the 189 cervical cancer samples, NR2F6 expression was positively related to International Federation of Gynecology and Obstetrics (FIGO stage (p = 0.006, squamous cell carcinoma antigen (p = 0.006, vital status (p < 0.001, tumor recurrence (p = 0.001, chemotherapy (p = 0.039, and lymph node metastasis (p < 0.001. Overall and disease-free survival was shorter in patients with early-stage cervical cancer and higher NR2F6 levels than in patients with lower levels of NR2F6. Univariate and multivariate analysis determined that NR2F6 was an independent prognostic factor of survival in early-stage cervical cancer. Conclusions: Taken together, our findings suggest that high NR2F6 expression predicts pelvic lymph node metastasis, tumor recurrence and poor prognosis in early-stage cervical cancer. NR2F6 might be a novel prognostic biomarker and potential therapeutic target of cervical cancer.

  13. Fluxes of total reactive atmospheric nitrogen (ΣNr using eddy covariance above arable land

    Directory of Open Access Journals (Sweden)

    Christophe R. Flechard

    2013-02-01

    Full Text Available The amount and timing of reactive nitrogen exchange between agricultural land and the atmosphere play a key role in evaluating ecosystem productivity and in addressing atmospheric nitrogen budgets and transport. With the recent development of the Total Reactive Atmospheric Nitrogen Converter (TRANC apparatus, a methodology has been provided for continuous measurement of the sum of all airborne nitrogen containing species (ΣNr allowing for diurnal and seasonal investigations. We present ΣNr concentration and net flux data from an 11-month field campaign conducted at an arable field using the TRANC system within an eddy-covariance setup. Clear diurnal patterns of both ΣNr concentrations and fluxes with significant dependencies on atmospheric stability and stomatal regulation were observed in the growing season. TRANC data were compared with monthly-averaged concentrations and dry deposition rates of selected Nr compounds using DELTA denuders and ensemble-averages of four inferential models, respectively. Similar seasonal trends were found for Nr concentrations from DELTA and TRANC measurements with values from the latter being considerably higher than those of DELTA denuders. The variability of the difference between these two systems could be explained by seasonally changing source locations of NOx contributions to the TRANC signal. As soil and vegetation Nr emissions to the atmosphere are generally not treated by inferential (dry deposition models, TRANC data showed lower monthly deposition rates than those obtained from inferential modelling. Net ΣNr exchange was almost neutral (~0.072 kg N ha−1 at the end of the observation period. However, during most parts of the year, slight but permanent net ΣNr deposition was found. Our measurements demonstrate that fertilizer addition followed by substantial ΣNr emissions plays a crucial role in a site's annual atmospheric nitrogen budget. As long-term Nr measurements with high temporal

  14. The orphan nuclear receptor NR4A2 is part of a p53?microRNA-34 network

    OpenAIRE

    Beard, Jordan A.; Tenga, Alexa; Hills, Justin; Hoyer, Jessica D.; Cherian, Milu T.; Wang, Yong-Dong; Chen, Taosheng

    2016-01-01

    Nuclear receptor subfamily 4 group A member 2 (NR4A2) is an orphan nuclear receptor that is over-expressed in cancer and promotes cell proliferation, migration, transformation, and chemoresistance. Increased expression and function of NR4A2 have been attributed to various signaling pathways, but little is known about microRNA (miRNA) regulation of NR4A2 in cancer. To investigate the posttranscriptional regulation of NR4A2, we used a 3? untranslated region (UTR) reporter screen and identified ...

  15. Targeting the NMDA receptor subunit NR2B for treating or preventing age-related memory decline.

    Science.gov (United States)

    Wang, Deheng; Jacobs, Stephanie A; Tsien, Joe Z

    2014-10-01

    Age-related memory loss is believed to be a result of reduced synaptic plasticity, including changes in the NR2 subunit composition of the NMDA receptor. It is known that endogenous NR2B subunits decrease as the brain ages, whereas transgenic upregulation of NR2B enhances synaptic plasticity and learning and memory in several animal species. Accumulating evidence suggests that elevated brain magnesium levels, via dietary supplementation, can boost NR2B in the brain, consequently reversing memory deficits and enhancing cognitive abilities. This review highlights the convergent molecular mechanisms via the NR2B pathway as a useful strategy for treating age-related memory loss. A dietary approach, via oral intake of a novel compound, magnesium L-threonate (MgT), to boost NR2B expression in the brain is highlighted. Direct upregulation of the NR2B subunit expression can enhance synaptic plasticity and memory functions in a broad range of behavioral tasks in rodents. Other upregulation approaches, such as targeting the NR2B transporter or surface recycling pathway via cyclin-dependent kinase 5, are highly effective in improving memory functions. A dietary supplemental approach by optimally elevating the [Mg²⁺] in the brain is surprisingly effective in upregulating NR2B expression and improving memories in preclinical studies. MgT is currently under clinical trials.

  16. Upregulation of protein phosphatase 2A and NR3A-pleiotropic effect of simvastatin on ischemic stroke rats.

    Directory of Open Access Journals (Sweden)

    Minxia Zhu

    Full Text Available Ca(2+ influxes are regulated by the functional state of N-methyl-D-aspartate receptors (NMDARs. Dephosphorylation of NMDARs subunits decreases Ca(2+ influxes. NR3, a novel subunit of NMDARs, also decreases Ca(2+ influxes by forming new NMDARs with NR1 and NR2. It is meaningful to uncover whether protein phosphatase 2A (PP2A and NR3A play a role in the protective effect of Simvastatin on ischemic stroke. In the present study, the Sprague-Dawley rats were pretreated with Simvastatin for 7 days before middle cerebral artery occlusion was performed to mimic ischemic stroke. The results showed that Simvastatin decreased brain ischemic infarct area significantly while increasing the expression levels of PP2A and NR3A, thus dephosphorylating the serine sites of NR1 (ser896 and ser897 along with increased enzymatic activities of PP2A. The protein levels of NR3A decreased as the enzymatic activities of PP2A were inhibited by okadaic acid. The results indicated that Simvastatin could protect the cerebrum from ischemic injury through a signaling mechanism involving elevated levels of PP2A and NR3A, and that PP2A might involve in the regulatory mechanism of NR3A expression.

  17. OBSERVAŢII REFERITOARE LA AMENDAMENTELE OPERATE ÎN CODUL PENAL PRIN LEGEA NR.60/2016

    Directory of Open Access Journals (Sweden)

    Vitalie STATI

    2016-11-01

    Full Text Available Obiectul prezentului studiu îl constituie modificările şi completările operate în Codul penal prin Legea nr.60/2016. Acestea vizează: persoana juridică în calitate de subiect al infracţiunii; confiscarea specială; spălarea banilor; actul terorist; finanţarea terorismului; abuzul de putere sau abuzul de serviciu. În procesul analizei, sunt examinate proiectul care se află la baza Legii nr.60/2016, Nota informativă la acest proiect şi alte asemenea acte preparatorii. De asemenea, se face referire la Convenţia privind spălarea, descoperirea, sechestrarea şi confiscarea produselor infracţiunii şi finan­ţarea terorismului, adoptată la Varşovia la 16.05.2005, precum şi la raportul explicativ la această Convenţie. Scopul prezentului studiu constă în stabilirea efectelor pozitive şi a celor negative ale adoptării modificărilor şi completărilor operate în Codul penal prin Legea nr.60/2016. În rezultatul investigaţiei efectuate se ajunge la concluzia că nu toate amendamentele operate prin legea amintită au tangenţă cu domeniile ce ţin de prevenirea şi combaterea spălării banilor şi a finanţării terorismului. Nu toate aceste amendamente pot asigura armonizarea legislaţiei naţionale penale cu stan­dardele stabilite de Curtea Europeană pentru Drepturile Omului. Unele din aceste amendamente sunt îndoielnice sub aspectul calităţii lor tehnico-legislative şi/sau în ce priveşte aportul lor în planul eficientizării apărării ordinii de drept. Impresia de ansamblu este că nu preocuparea pentru interesul social a constituit scopul principal al adoptării Legii nr.60/2016.SOME REMARKS ON THE AMENDMENTS OPERATED TO THE PENAL CODE BY LAW No.60/2016The object of this study is formed by the modifications and additions operated to the Penal Code by Law No.60/2016. The content of the respective initiatives addresses the following elements: the legal person as the subject of the offence; special confiscation

  18. Effect of NMDA NR2B antagonist on neuropathic pain in two spinal cord injury models.

    Science.gov (United States)

    Kim, Youngkyung; Cho, Hwi-young; Ahn, Young Ju; Kim, Junesun; Yoon, Young Wook

    2012-05-01

    N-Methyl-d-aspartate (NMDA) receptors are thought to play an important role in the processes of central sensitization and pathogenesis of neuropathic pain, particularly after spinal cord injury (SCI). NMDA antagonists effectively reduce neuropathic pain, but serious side effects prevent their use as therapeutic drugs. NMDA NR2B antagonists have been reported to effectively reduce inflammatory and neuropathic pain. In this study, we investigated the effects of NR2B antagonists on neuropathic pain and the expression of NR2B in the spinal cord in 2 SCI models. SCI was induced at T12 by a New York University impactor (contusion) or by sectioning of the lateral half of the spinal cord (hemisection). Ifenprodil (100, 200, 500, 1000nmol) and Ro25-6981 (20, 50, 100, 200nmol) were intrathecally injected and behavioral tests were conducted. Ifenprodil increased the paw withdrawal threshold in both models but also produced mild motor depression at higher doses. Ro25-6981 increased the mechanical nociceptive threshold in a dose-dependent manner without motor depression. NR2B expression was significantly increased on both sides at the spinal segments of L1-2 and L4-5 in the hemisection model but did not change in the contusion model. Increased expression of NR2B in the hemisection model was reduced by intrathecal ifenprodil. These results suggest that intrathecal NMDA NR2B antagonist increased the mechanical nociceptive threshold after SCI without motor depression. A selective subtype of NMDA receptor, such as NR2B, may be a more selective target for pain control because NMDA receptors play a crucial role in the development and maintenance of chronic pain. Copyright © 2012 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

  19. Pharmacological isolation of postsynaptic currents mediated by NR2A- and NR2B-containing NMDA receptors in the anterior cingulate cortex

    Directory of Open Access Journals (Sweden)

    Cao Xiaoyan

    2007-04-01

    Full Text Available Abstract NMDA receptors (NMDARs are involved in excitatory synaptic transmission and plasticity associated with a variety of brain functions, from memory formation to chronic pain. Subunit-selective antagonists for NMDARs provide powerful tools to dissect NMDAR functions in neuronal activities. Recently developed antagonist for NR2A-containing receptors, NVP-AAM007, triggered debates on its selectivity and involvement of the NMDAR subunits in bi-directional synaptic plasticity. Here, we re-examined the pharmacological properties of NMDARs in the anterior cingulate cortex (ACC using NVP-AAM007 as well as ifenprodil, a selective antagonist for NR2B-containing NMDARs. By alternating sequence of drug application and examining different concentrations of NVP-AAM007, we found that the presence of NVP-AAM007 did not significantly affect the effect of ifenprodil on NMDAR-mediated EPSCs. These results suggest that NVP-AAM007 shows great preference for NR2A subunit and could be used as a selective antagonist for NR2A-containing NMDARs in the ACC.

  20. Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov., isolated from the honey stomach of the honeybee Apis mellifera

    Science.gov (United States)

    Alsterfjord, Magnus; Nilson, Bo; Butler, Èile; Vásquez, Alejandra

    2014-01-01

    We previously discovered a symbiotic lactic acid bacterial (LAB) microbiota in the honey stomach of the honeybee Apis mellifera. The microbiota was composed of several phylotypes of Bifidobacterium and Lactobacillus. 16S rRNA gene sequence analyses and phenotypic and genetic characteristics revealed that the phylotypes isolated represent seven novel species. One grouped with Lactobacillus kunkeei and the others belong to the Lactobacillus buchneri and Lactobacillus delbrueckiisubgroups of Lactobacillus. We propose the names Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov. for these novel species, with the respective type strains being Fhon13NT ( = DSM 26257T = CCUG 63287T), Bin4NT ( = DSM 26254T = CCUG 63291T), Hon2NT ( = DSM 26255T = CCUG 63289T), Hma8NT ( = DSM 26256T = CCUG 63629T), Hma2NT ( = DSM 26263T = CCUG 63633T), Bma5NT ( = DSM 26265T = CCUG 63301T) and Biut2NT ( = DSM 26262T = CCUG 63631T). PMID:24944337

  1. Ecdysteroids from a Zoanthus sp.

    Science.gov (United States)

    Suksamrarn, Apichart; Jankam, Aroon; Tarnchompoo, Bongkoch; Putchakarn, Sumaitt

    2002-08-01

    A new ecdysteroid, zoanthusterone, has been isolated from a marine zoanthid, Zoanthus sp. Ten known ecdysteroids, ponasterone A, 20-hydroxyecdysone 2-acetate, viticosterone E, integristerone A 25-acetate, 2-deoxy-20-hydroxyecdysone, ecdysone, ajugasterone C, dacryhainansterone, inokosterone, and 20-hydroxyecdysone, have also been isolated. This is the first report of ecdysteroids in a Zoanthus species.

  2. Sporulation of Cyclospora sp. oocysts.

    OpenAIRE

    Smith, H V; Paton, C A; Mitambo, M M; Girdwood, R W

    1997-01-01

    Cyclospora sp. oocysts sporulated maximally at 22 and 30 degrees C for 14 days retarded sporulation. Up to 12% of human- and baboon-derived oocysts previously stored at 4 degrees C for 1 to 2 months sporulated when stored for 6 to 7 days at 30 degrees C.

  3. Identification of NR4A2 as a transcriptional activator of IL-8 expression in human inflammatory arthritis.

    LENUS (Irish Health Repository)

    Aherne, Carol M

    2009-10-01

    Expression of the orphan nuclear receptor NR4A2 is controlled by pro-inflammatory mediators, suggesting that NR4A2 may contribute to pathological processes in the inflammatory lesion. This study identifies the chemoattractant protein, interleukin 8 (IL-8\\/CXCL8), as a molecular target of NR4A2 in human inflammatory arthritis and examines the mechanism through which NR4A2 modulates IL-8 expression. In TNF-alpha-activated human synoviocyte cells, enhanced expression of IL-8 mRNA and protein correspond to temporal changes in NR4A2 transcription and nuclear distribution. Ectopic expression of NR4A2 leads to robust changes in endogenous IL-8 mRNA levels and co-treatment with TNF-alpha results in significant (p<0.001) secretion of IL-8 protein. Transcriptional effects of NR4A2 on the human IL-8 promoter are enhanced in the presence of TNF-alpha, suggesting molecular crosstalk between TNF-alpha signalling and NR4A2. A dominant negative IkappaB kinase antagonizes the combined effects of NR4A2 and TNF-alpha on IL-8 promoter activity. Co-expression of NR4A2 and the p65 subunit of NF-kappaB enhances IL-8 transcription and functional studies indicate that transactivation occurs independently of NR4A2 binding to DNA or heterodimerization with additional nuclear receptors. The IL-8 minimal promoter region is sufficient to support NR4A2 and NF-kappaB\\/p65 co-operative activity and NR4A2 can interact with NF-kappaB\\/p65 on a 39bp sequence within this region. In patients treated with methotrexate for active inflammatory arthritis, a reduction in NR4A2 synovial tissue levels correlate significantly (n=10, r=0.73, p=0.002) with changes in IL-8 expression. Collectively, these data delineate an important role for NR4A2 in modulating IL-8 expression and reveal novel transcriptional responses to TNF-alpha in human inflammatory joint disease.

  4. Pilot scale experiments on radiation vulcanization of NR latex

    Science.gov (United States)

    Ridwan, M.

    The potential of irradiated latex as raw material of commercial use is under testing on pilot plant scale in Indonesia which has 225 kCi Co-60 irradiation facility and can irradiate 1000 tonnes of centrifuged latex per annum. The facility was jointly designed by BATAN of Indonesia and JAERI of Japan and was jointly financed by UNDP/IAEA, Government of Japan and Government of Indonesia under UNDP/IAEA Regional Cooperative Agreement Project on Industrial Application of Isotopes and Radiation Technology. The facility is a water pool type and can accomodate 400 kCi Co-60. The Co-60 rack has two shapes, plate and cylindrical shapes. The plate shape source is used for natural rubber latex irradiation and the cylindrical one is used for other irradiation services. The vulcanization system consists of three major components : emulsification unit ( height : 650 mm, diameter 500 mm ), mixing unit ( height : 1900mm, diameter 1200 mm ) and vulcanization reactor ( height : 1800 mm, diameter 1300 mm ). The first two components are located outside shielded room while the third one-in irradiation room. The radiation vulcanization process is a much simpler energy saving process comparedto the conventional thermal process which has two vulcanization steps before and after dipping. The physical and mechanical properties of irradiated NR Latex are comparable to those of sulfur vulcanized, and depend on many factors such as irradiation dose, sensitizer content, dry rubber content and storage time.

  5. Pilot scale experiments on radiation vulcanization of NR latex

    International Nuclear Information System (INIS)

    Ridwan, M.

    1985-01-01

    The potential of irradiated latex as raw material of commercial use is under testing on pilot plant scale in Indonesia which has 225 kCi Co-60 irradiation facility and can irradiate 1000 tonnes of centrifuged latex per annum. The facility was jointly designed by BATAN of Indonesia and JAERI of Japan and was jointly financed by UNDP/IAEA, Government of Japan and Government of Indonesia under UNDP/IAEA Regional Cooperative Agreement Project on Industrial Application of Isotopes and Radiation Technology. The facility is a water pool type and can accommodate 400 kCi Co-60. The Co-60 rack has two shapes, plate and cylindrical shapes. The plate shape source is used for natural rubber latex irradiation and the cylindrical one is used for other irradiation services. The vulcanization system consists of three major components: emulsification unit, mixing unit and vulcanization reactor. The first two components are located outside shielded room while the third one in irradiation room. The radiation vulcanization process is a much simpler energy saving process compared to the conventional thermal process which has two vulcanization steps before and after dipping. The physical and mechanical properties of irradiated NR latex are comparable to those of sulfur vulcanized. (author)

  6. Both NR2A and NR2B Subunits of the NMDA Receptor Are Critical for Long-Term Potentiation and Long-Term Depression in the Lateral Amygdala of Horizontal Slices of Adult Mice

    Science.gov (United States)

    Muller, Tobias; Albrecht, Doris; Gebhardt, Christine

    2009-01-01

    The lateral nucleus of the amygdala (LA) is implicated in emotional and social behaviors. We recently showed that in horizontal brain slices, activation of NMDA receptors (NMDARs) is a requirement for persistent synaptic alterations in the LA, such as long-term potentiation (LTP) and long-term depression (LTD). In the LA, NR2A- and NR2B-type NMDRs…

  7. Stenhammer, Wilhelm. sinfonien Nr. 1 F-Dur und Nr. 2 g-Moll op. 34, Excelsior! op. 13, Serenade F-Dur op. 31. Sinfonieorchester Göteborg, Neeme Järvi / Andreas K. W. Meyer

    Index Scriptorium Estoniae

    Meyer, Andreas K. W.

    1995-01-01

    Uuest heliplaadist "Stenhammer, Wilhelm. sinfonien Nr. 1 F-Dur und Nr. 2 g-Moll op. 34, Excelsior! op. 13, Serenade F-Dur op. 31. Sinfonieorchester Göteborg, Neeme Järvi". DG 2 CD 445857-2 (WD: 138'37")

  8. The role of NR4A transcription factors in memory formation.

    Science.gov (United States)

    Hawk, Josh D; Abel, Ted

    2011-04-25

    In various physiological contexts, Nr4a genes are transcribed in response to external stimuli as part of an immediate early response that initiates a cascade of gene expression ultimately leading to distinct physiological outcomes in each of these contexts. The signaling pathway that initiates Nr4a gene expression in most of these contexts consists of elevated intracellular cAMP activating PKA, which in turn leads to phosphorylation of CREB and new gene synthesis. This cAMP-PKA-CREB pathway is a central molecular pathway in the formation of a long-term memory. Indeed, learning induces Nr4a family gene expression, and long-term memory formation requires at least two waves of transcription after learning, suggesting that NR4A nuclear receptors may contribute to the second of these waves of gene expression. In this article, we review insights gained in other physiological contexts regarding Nr4a function and regulation, and highlight how these lessons can be applied to the study of memory formation. Copyright © 2011 Elsevier Inc. All rights reserved.

  9. An Sp1/Sp3 binding polymorphism confers methylation protection.

    Directory of Open Access Journals (Sweden)

    Yanis A Boumber

    2008-08-01

    Full Text Available Hundreds of genes show aberrant DNA hypermethylation in cancer, yet little is known about the causes of this hypermethylation. We identified RIL as a frequent methylation target in cancer. In search for factors that influence RIL hypermethylation, we found a 12-bp polymorphic sequence around its transcription start site that creates a long allele. Pyrosequencing of homozygous tumors revealed a 2.1-fold higher methylation for the short alleles (P<0.001. Bisulfite sequencing of cancers heterozygous for RIL showed that the short alleles are 3.1-fold more methylated than the long (P<0.001. The comparison of expression levels between unmethylated long and short EBV-transformed cell lines showed no difference in expression in vivo. Electrophorectic mobility shift assay showed that the inserted region of the long allele binds Sp1 and Sp3 transcription factors, a binding that is absent in the short allele. Transient transfection of RIL allele-specific transgenes showed no effects of the additional Sp1 site on transcription early on. However, stable transfection of methylation-seeded constructs showed gradually decreasing transcription levels from the short allele with eventual spreading of de novo methylation. In contrast, the long allele showed stable levels of expression over time as measured by luciferase and approximately 2-3-fold lower levels of methylation by bisulfite sequencing (P<0.001, suggesting that the polymorphic Sp1 site protects against time-dependent silencing. Our finding demonstrates that, in some genes, hypermethylation in cancer is dictated by protein-DNA interactions at the promoters and provides a novel mechanism by which genetic polymorphisms can influence an epigenetic state.

  10. NR2B-containing NMDA receptors promote neural progenitor cell proliferation through CaMKIV/CREB pathway

    Energy Technology Data Exchange (ETDEWEB)

    Li, Mei, E-mail: limeihit@163.com [Department of Anatomy and Neurobiology, Xuzhou Medical College, Xuzhou (China); Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing (China); Zhang, Dong-Qing; Wang, Xiang-Zhen [Department of Anatomy and Neurobiology, Xuzhou Medical College, Xuzhou (China); Xu, Tie-Jun, E-mail: xztjxu@163.com [Department of Anatomy and Neurobiology, Xuzhou Medical College, Xuzhou (China); Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing (China)

    2011-08-12

    Highlights: {yields} The NR2B component of the NMDARs is important for the NSPC proliferation. {yields} pCaMKIV and pCREB exist in NSPCs. {yields} The CaMKIV/CREB pathway mediates NSPC proliferation. -- Abstract: Accumulating evidence indicates the involvement of N-methyl-D-aspartate receptors (NMDARs) in regulating neural stem/progenitor cell (NSPC) proliferation. Functional properties of NMDARs can be markedly influenced by incorporating the regulatory subunit NR2B. Here, we aim to analyze the effect of NR2B-containing NMDARs on the proliferation of hippocampal NSPCs and to explore the mechanism responsible for this effect. NSPCs were shown to express NMDAR subunits NR1 and NR2B. The NR2B selective antagonist, Ro 25-6981, prevented the NMDA-induced increase in cell proliferation. Moreover, we demonstrated that the phosphorylation levels of calcium/calmodulin-dependent protein kinase IV (CaMKIV) and cAMP response element binding protein (CREB) were increased by NMDA treatment, whereas Ro 25-6981 decreased them. The role that NR2B-containing NMDARs plays in NSPC proliferation was abolished when CREB phosphorylation was attenuated by CaMKIV silencing. These results suggest that NR2B-containing NMDARs have a positive role in regulating NSPC proliferation, which may be mediated through CaMKIV phosphorylation and subsequent induction of CREB activation.

  11. NR1I2 polymorphisms are related to tacrolimus dose-adjusted exposure and BK viremia in adult kidney transplantation

    DEFF Research Database (Denmark)

    Barraclough, Katherine A; Isbel, Nicole M; Lee, Katie J

    2012-01-01

    BACKGROUND: Pregnane X, encoded by the gene NR112, is a nuclear receptor whose primary role is to promote the detoxification and clearance of drugs and other foreign compounds from the body. AIM: The aim of this study was to analyze associations between NR1I2 polymorphisms, immunosuppressant drug...

  12. Limited contribution of NR5A1 (SF-1) mutations in women with primary ovarian insufficiency (POI)

    NARCIS (Netherlands)

    Janse, F.; de With, L.M.; Duran, KJ; Kloosterman, W.P.; Goverde, A.J.; Lambalk, C.B.; Laven, J.S.E.; Fauser, B.C.J.M.; Giltay, J.C.

    2012-01-01

    Objective: To evaluate the significance of NR5A1 mutations in a large, well-phenotyped cohort of women with primary ovarian insufficiency (POI). Mutations in the NR5A1 gene (SF-1) were previously described in disorders of sexual development and adrenal insufficiency. Recently, a high frequency of

  13. Distinct expression of synaptic NR2A and NR2B in the central nervous system and impaired morphine tolerance and physical dependence in mice deficient in postsynaptic density-93 protein

    Directory of Open Access Journals (Sweden)

    Johns Roger A

    2008-10-01

    Full Text Available Abstract Postsynaptic density (PSD-93, a neuronal scaffolding protein, binds to and clusters N-methyl-D-aspartate receptor (NMDAR subunits NR2A and NR2B at cellular membranes in vitro. However, the roles of PSD-93 in synaptic NR2A and NR2B targeting in the central nervous system and NMDAR-dependent physiologic and pathologic processes are still unclear. We report here that PSD-93 deficiency significantly decreased the amount of NR2A and NR2B in the synaptosomal membrane fractions derived from spinal cord dorsal horn and forebrain cortex but did not change their levels in the total soluble fraction from either region. However, PSD-93 deficiency did not markedly change the amounts of NR2A and NR2B in either synaptosomal or total soluble fractions from cerebellum. In mice deficient in PSD-93, morphine dose-dependent curve failed to shift significantly rightward as it did in wild type (WT mice after acute and chronic morphine challenge. Unlike WT mice, PSD-93 knockout mice also showed marked losses of NMDAR-dependent morphine analgesic tolerance and associated abnormal sensitivity in response to mechanical, noxious thermal, and formalin-induced inflammatory stimuli after repeated morphine injection. In addition, PSD-93 knockout mice displayed dramatic loss of jumping activity, a typical NMDAR-mediated morphine withdrawal abstinence behavior. These findings indicate that impaired NMDAR-dependent neuronal plasticity following repeated morphine injection in PSD-93 knockout mice is attributed to PSD-93 deletion-induced alterations of synaptic NR2A and NR2B expression in dorsal horn and forebrain cortex neurons. The selective effect of PSD-93 deletion on synaptic NMDAR expression in these two major pain-related regions might provide the better strategies for the prevention and treatment of opioid tolerance and physical dependence.

  14. Antioxidant capacity, polyphenol content and iron bioavailability from algae (Ulva sp., Sargassum sp. and Porphyra sp.) in human subjects.

    Science.gov (United States)

    García-Casal, Maria N; Ramírez, José; Leets, Irene; Pereira, Ana C; Quiroga, Maria F

    2009-01-01

    Marine algae are easily produced and are good sources of Fe. If this Fe is bioavailable, algae consumption could help to combat Fe deficiency and anaemia worldwide. The objective of the present study was to evaluate Fe bioavailability, polyphenol content and antioxidant capacity from three species of marine algae distributed worldwide. A total of eighty-three subjects received maize- or wheat-based meals containing marine algae (Ulva sp., Sargassum sp. and Porphyra sp.) in different proportions (2.5, 5.0 and 7.5 g) added to the water to prepare the dough. All meals administered contained radioactive Fe. Absorption was evaluated calculating radioactive Fe incorporation in subjects' blood. The three species of marine algae were analysed for polyphenol content and reducing power. Algae significantly increased Fe absorption in maize- or wheat-based meals, especially Sargassum sp., due to its high Fe content. Increases in absorption were dose-dependent and higher in wheat- than in maize-based meals. Total polyphenol content was 10.84, 18.43 and 80.39 gallic acid equivalents/g for Ulva sp., Porphyra sp. and Sargassum sp., respectively. The antioxidant capacity was also significantly higher in Sargassum sp. compared with the other two species analysed. Ulva sp., Sargassum sp. and Porphyra sp. are good sources of bioavailable Fe. Sargassum sp. resulted in the highest Fe intake due to its high Fe content, and a bread containing 7.5 g Sargassum sp. covers daily Fe needs. The high polyphenol content found in Sargassum sp. could be partly responsible for the antioxidant power reported here, and apparently did not affect Fe absorption.

  15. Iterative use of nuclear receptor Nr5a2 regulates multiple stages of liver and pancreas development.

    Science.gov (United States)

    Nissim, Sahar; Weeks, Olivia; Talbot, Jared C; Hedgepeth, John W; Wucherpfennig, Julia; Schatzman-Bone, Stephanie; Swinburne, Ian; Cortes, Mauricio; Alexa, Kristen; Megason, Sean; North, Trista E; Amacher, Sharon L; Goessling, Wolfram

    2016-10-01

    The stepwise progression of common endoderm progenitors into differentiated liver and pancreas organs is regulated by a dynamic array of signals that are not well understood. The nuclear receptor subfamily 5, group A, member 2 gene nr5a2, also known as Liver receptor homolog-1 (Lrh-1) is expressed in several tissues including the developing liver and pancreas. Here, we interrogate the role of Nr5a2 at multiple developmental stages using genetic and chemical approaches and uncover novel pleiotropic requirements during zebrafish liver and pancreas development. Zygotic loss of nr5a2 in a targeted genetic null mutant disrupted the development of the exocrine pancreas and liver, while leaving the endocrine pancreas intact. Loss of nr5a2 abrogated exocrine pancreas markers such as trypsin, while pancreas progenitors marked by ptf1a or pdx1 remained unaffected, suggesting a role for Nr5a2 in regulating pancreatic acinar cell differentiation. In the developing liver, Nr5a2 regulates hepatic progenitor outgrowth and differentiation, as nr5a2 mutants exhibited reduced hepatoblast markers hnf4α and prox1 as well as differentiated hepatocyte marker fabp10a. Through the first in vivo use of Nr5a2 chemical antagonist Cpd3, the iterative requirement for Nr5a2 for exocrine pancreas and liver differentiation was temporally elucidated: chemical inhibition of Nr5a2 function during hepatopancreas progenitor specification was sufficient to disrupt exocrine pancreas formation and enhance the size of the embryonic liver, suggesting that Nr5a2 regulates hepatic vs. pancreatic progenitor fate choice. Chemical inhibition of Nr5a2 at a later time during pancreas and liver differentiation was sufficient to block the formation of mature acinar cells and hepatocytes. These findings define critical iterative and pleiotropic roles for Nr5a2 at distinct stages of pancreas and liver organogenesis, and provide novel perspectives for interpreting the role of Nr5a2 in disease. Copyright © 2016

  16. Microstructure, molecular weight and thermal behavior of natural rubber (NR) from mangabeira (Hancornia speciosa)

    International Nuclear Information System (INIS)

    Santos, Expedito Flavio dos; Feitosa, Judith P.A.; Ricardo, Nagila M.P.S.

    2001-01-01

    The natural rubber (NR) from Hancornia speciosa contains characteristics that turns it a good alternative in elastomers supply. The NMR and IR spectra showed that the rubber of mangabeira is composed fundamentally by poly(1,4-cis-isoprene). The rubber molecular weight obtained by GPC and viscometry was 2,0x10 6 and 1,3x10 6 g/mol, respectively, in good agreement with the values determined for seringueira and manicoba NR. The glass transition temperature obtained by DSC (Tg = - 65 deg C) showed the mangabeira rubber is ideal to be utilized in regions of cold climate without compromising its mechanical properties. The rubber has also good thermal stability up to 213 deg C, as indicated by TG curves. This results indicated that the mangabeira NR can be effectively used in vulcanized articles or to be added to asphalt. (author)

  17. Pharmacological Activators of the NR4A Nuclear Receptors Enhance LTP in a CREB/CBP-Dependent Manner.

    Science.gov (United States)

    Bridi, Morgan S; Hawk, Joshua D; Chatterjee, Snehajyoti; Safe, Stephen; Abel, Ted

    2017-05-01

    Nr4a nuclear receptors contribute to long-term memory formation and are required for long-term memory enhancement by a class of broad-acting drugs known as histone deacetylase (HDAC) inhibitors. Understanding the molecular mechanisms that regulate these genes and identifying ways to increase their activity may provide novel therapeutic approaches for ameliorating cognitive dysfunction. In the present study, we find that Nr4a gene expression after learning requires the cAMP-response element binding (CREB) interaction domain of the histone acetyltransferase CREB-binding protein (CBP). These gene expression deficits emerge at a time after learning marked by promoter histone acetylation in wild-type mice. Further, mutation of the CREB-CBP interaction domain reduces Nr4a promoter acetylation after learning. As memory enhancement by HDAC inhibitors requires CREB-CBP interaction and Nr4a gene function, these data support the notion that the balance of histone acetylation at the Nr4a promoters is critical for memory formation. NR4A ligands have recently been described, but the effect of these drugs on synaptic plasticity or memory has not been investigated. We find that the 'C-DIM' NR4A ligands, para-phenyl substituted di-indolylmethane compounds, enhance long-term contextual fear memory and increase the duration of long-term potentiation (LTP), a form of hippocampal synaptic plasticity. LTP enhancement by these drugs is eliminated in mice expressing a dominant negative form of NR4A and attenuated in mice with mutation of the CREB-CBP interaction domain. These data define the molecular connection between histone acetylation and Nr4a gene expression after learning. In addition, they suggest that NR4A-activating C-DIM compounds may serve as a potent and selective means to enhance memory and synaptic plasticity.

  18. Phase topology of a NR/BR elastomer blend with active filler

    Directory of Open Access Journals (Sweden)

    Plavšić Milenko B.

    2003-01-01

    Full Text Available The relations between the structure and mechanical properties of a polymer blend of natural (NR and polybutadiene (BR rubber (i.e. a NR/BR blend with the weight ratio of the components 70/30 filled with active carbon black were analysed. The properties of the individual phases in the blend were resolved by modeling the stress-strain relationship according to the Bauer procedure for high extensions. The obtained results indicated that BR is the dispersed phase, having a higher modulus, which was also confirmed by the much better fit of the experimental data to the series type of phase coupling according to the Takanayagy theory.

  19. Pantoea rodasii sp. nov., Pantoea rwandensis sp. nov. and Pantoea wallisii sp. nov., isolated from Eucalyptus.

    Science.gov (United States)

    Brady, Carrie L; Cleenwerck, Ilse; van der Westhuizen, Lorinda; Venter, Stephanus N; Coutinho, Teresa A; De Vos, Paul

    2012-07-01

    Several Gram-negative-staining, facultatively anaerobic bacterial isolates were obtained from Eucalyptus seedlings showing symptoms of bacterial blight and dieback in Colombia, Rwanda and South Africa. Partial 16S rRNA gene sequencing, together with partial gyrB sequencing, placed the isolates in the genus Pantoea and indicated that they constituted three novel species. Multilocus sequence analysis (MLSA) based on partial sequences of gyrB, rpoB, infB and atpD revealed Pantoea dispersa, Pantoea eucrina and Pantoea cypripedii as their closest phylogenetic relatives. DNA-DNA hybridization studies confirmed the classification of the new isolates as three novel species and phenotypic tests allowed them to be differentiated from their closest phylogenetic neighbours. The names Pantoea rodasii sp. nov. [type strain LMG 26273(T)=BD 943(T) (deposited with the Plant Pathogenic and Plant Protecting Bacteria Collection, South Africa)=BCC 581(T) (deposited with the Bacterial Culture Collection, Forestry and Agricultural Institute, South Africa)], Pantoea rwandensis sp. nov. (type strain LMG 26275(T)=BD 944(T)=BCC 571(T)) and Pantoea wallisii sp. nov. (type strain LMG 26277(T)=BD 946(T)=BCC 682(T)) are proposed.

  20. Overexpression of the NR2A subunit in the forebrain impairs long-term social recognition and non-social olfactory memory.

    Science.gov (United States)

    Jacobs, S A; Tsien, J Z

    2014-04-01

    Animals must recognize and remember conspecifics and potential mates, and distinguish these animals from potential heterospecific competitors and predators. Despite its necessity, aged animals are known to exhibit impaired social recognition memory. As the brain ages, the ratio of NR2A:NR2B in the brain increases over time and has been postulated to underlie the cognitive decline observed during the aging process. Here, we test the hypothesis that an increased NR2A:NR2B subunit ratio underlies long-term social recognition memory. Using transgenic overexpression of NR2A in the forebrain regions, we investigated the ability of these mice to learn and remember male and female conspecifics, mice of another strain and animals of another rodent species, the rat. Furthermore, due to the importance of olfaction in social recognition, we tested the olfactory memory in the NR2A transgenic mice. Our series of behavioral experiments revealed significant impairments in the NR2A transgenic mice in long-term social memory of both male and female conspecifics. Additionally, the NR2A transgenic mice are unable to recognize mice of another strain or rats. The NR2A transgenic mice also exhibited long-term memory impairments in the olfactory recognition task. Taken together, our results provide evidence that an increased NR2A:NR2B ratio in the forebrain leads to reduced long-term memory function, including the ethologically important memories such as social recognition and olfactory memory.

  1. DISTRIBUSI Solen sp DI PERAIRAN KABUPATEN BANGKALAN

    Directory of Open Access Journals (Sweden)

    Eva Ari Wahyuni

    2016-03-01

    Full Text Available DISTRIBUTION OF Solen sp IN BANGKALAN WATERSSolen sp potential needs to be developed on the island of Madura, particularly in Bangkalan. Solen sp utilization has increased which has the potential to overfishing. Therefore, this study aims to determine the density of Solen sp and their ecology in the waters Modung village, Modung District, Bangkalan. The experiment was conducted in April 2015 using the descriptive method. The materials used include Solen sp and physico-chemical parameters of the environment (temperature, salinity, pH, and substrate. The analyzes were conducted at the Laboratory of Marine Science, Department of Marine Sciences, Trunojoyo University of Madura by using the tool grabsampler, sieveshaker, and pipetting with gravimetric method. The analysis shows the range of values of temperature between 29-300C, salinity between 31-32 ppt, pH were 7.9-8.0 and the type of substrate in the form of sandy mud, as well as the density of Solen sp from 8-10 individuals/m2. All measurement results indicate normal conditions and in accordance with the sea water quality standard for marine life, which can be a suitable habitat for the growth and development of Solen sp. This condition is thought to affect the density of Solen sp.Keywords: Bangkalan, density, distribution, Solen sp, substrate.ABSTRAKPotensi Solen sp perlu dikembangkan di pulau Madura, khususnya di Kabupaten Bangkalan. Pemanfaatan Solen sp mengalami peningkatan sehingga berpotensi overfishing. Untuk itu, penelitian ini bertujuan untuk mengetahui kepadatan Solen sp dan ekologinya di perairan desa Modung, Kecamatan Modung, Kabupaten Bangkalan. Penelitian dilaksanakan pada bulan April 2015 dengan metode deskriptif. Materi dan bahan yang digunakan diantaranya Solen sp dan parameter fisika-kimia lingkungan (suhu, salinitas, pH, dan substrat. Analisa dilakukan di Laboratorium Ilmu Kelautan, Program studi/Jurusan Ilmu Kelautan Universitas Trunojoyo Madura dengan menggunakan alat

  2. Influence of acetone extract from natural rubber on the structure and interface interaction in NR/silica composites

    Science.gov (United States)

    Xu, Tiwen; Jia, Zhixin; Wu, Lianghui; Chen, Yongjun; Luo, Yuanfang; Jia, Demin; Peng, Zheng

    2017-11-01

    It is well known that the coupling reagents as the additional modifiers were often used to improve the reinforcement effect of silica filled natural rubber. Actually, the commercial raw NR is a mixture consisting of polyisoprene and non-isoprene, where the latter one might have impact on the properties of NR/silica composites as an inartificial modifier inside. Thus, investigating the effect of non-isoprene compounds on the structure and properties of NR/silica composites is a novel approach to disclose the peculiarity of NR, which is meaningful to the assessment of NR quality. In this paper, the influences of acetone extract (AE) from natural rubber on the structure and mechanical properties of NR/silica composites were studied. Then the interfacial interactions between AE and silica were also illustrated through Fourier transform infrared spectroscopy (FTIR), thermogravimetic analysis (TGA), X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). Results demonstrated the existence of hydrogen bond between silica and AE, also the covalent bond induced by esterification reaction between sbnd COOH and Sisbnd OH, which resulted in an increase of constrained regions around silica surface leading to the promotions on mechanical and dynamical properties of NR/silica composites significantly.

  3. Behavioral analysis of NR2C knockout mouse reveals deficit in acquisition of conditioned fear and working memory.

    Science.gov (United States)

    Hillman, Brandon G; Gupta, Subhash C; Stairs, Dustin J; Buonanno, Andres; Dravid, Shashank M

    2011-05-01

    N-methyl-D-aspartate (NMDA) receptors play an important role in excitatory neurotransmission and mediate synaptic plasticity associated with learning and memory. NMDA receptors are composed of two NR1 and two NR2 subunits and the identity of the NR2 subunit confers unique electrophysiologic and pharmacologic properties to the receptor. The precise role of NR2C-containing receptors in vivo is poorly understood. We have performed a battery of behavioral tests on NR2C knockout/nβ-galactosidase knock-in mice and found no difference in spontaneous activity, basal anxiety, forced-swim immobility, novel object recognition, pain sensitivity and reference memory in comparison to wildtype counterparts. However, NR2C knockout mice were found to exhibit deficits in fear acquisition and working memory compared to wildtype mice. Deficit in fear acquisition correlated with lack of fear conditioning-induced plasticity at the thalamo-amygdala synapse. These findings suggest a unique role of NR2C-containing receptors in associative and executive learning representing a novel therapeutic target for deficits in cognition. Copyright © 2011 Elsevier Inc. All rights reserved.

  4. A role of periaqueductal grey NR2B-containing NMDA receptor in mediating persistent inflammatory pain

    Directory of Open Access Journals (Sweden)

    Yang Qi

    2009-12-01

    Full Text Available Abstract The midbrain periaqueductal grey (PAG is a structure known for its roles in pain transmission and modulation. Noxious stimuli potentiate the glutamate synaptic transmission and enhance glutamate NMDA receptor expression in the PAG. However, little is known about roles of NMDA receptor subunits in the PAG in processing the persistent inflammatory pain. The present study was undertaken to investigate NR2A- and NR2B-containing NMDA receptors in the PAG and their modulation to the peripheral painful inflammation. Noxious stimuli induced by hind-paw injection of complete Freund's adjuvant (CFA caused up-regulation of NR2B-containing NMDA receptors in the PAG, while NR2A-containing NMDA receptors were not altered. Whole-cell patch-clamp recordings revealed that NMDA receptor mediated mEPSCs were increased significantly in the PAG synapse during the chronic phases of inflammatory pain in mice. PAG local infusion of Ro 25-6981, an NR2B antagonist, notably prolonged the paw withdrawal latency to thermal radian heat stimuli bilaterally in rats. Hyperoside (Hyp, one of the flavonoids compound isolated from Rhododendron ponticum L., significantly reversed up-regulation of NR2B-containing NMDA receptors in the PAG and exhibited analgesic activities against persistent inflammatory stimuli in mice. Our findings provide strong evidence that up-regulation of NR2B-containing NMDA receptors in the PAG involves in the modulation to the peripheral persistent inflammatory pain.

  5. Determinação do teor de NR/SBR em misturas: associação de dados DTG e FT-IR Determination of NR/SBR content in blends: combining DTG and FT-IR data

    Directory of Open Access Journals (Sweden)

    Rita C. L. Dutra

    2004-12-01

    Full Text Available Misturas contendo teor conhecido de borracha natural (NR e copolímero de butadieno e estireno (SBR foram preparadas nos laboratórios do CTA e do IFOCA como amostras de referência para a elaboração de uma curva analítica, visando à determinação do teor de NR e SBR por análise pirólise/FT-IR. Termogravimetria foi usada para determinar o teor real de NR e SBR nas misturas por meio da razão entre as alturas dos picos da curva derivada (DTG. As bandas FT-IR escolhidas na região MIR foram 885 cm-1 (A1 para NR e 699 cm-1 (A2 para SBR. Os valores de absorvância relativa (A1/A2 versus a relação NR/SBR, obtida a partir dos dados fornecidos pela análise DTG, foram usados para construir uma curva com uma boa correlação linear (R=0,998, a qual possibilita determinar os teores de NR e SBR em misturas onde os componentes têm a mesma composição química.Blends with known contents of natural rubber (NR and butadiene-styrene copolymer (SBR were prepared at CTA and IFOCA laboratories as sample references for producing an analytical curve to determine the contents of NR and SBR via pyrolysis/FT-IR analysis. Thermogravimetry was used to quantify the real content of NR and SBR in the blends through the ratio between the intensity of the peaks in the derivative curve (DTG. The FT-IR analytical bands chosen at MIR region were 885 cm-1 (A1 for NR and 699 cm-1 (A2 for SBR. Values of relative absorbance (A1/A2 versus the NR/SBR contents data obtained from DTG analysis were used to construct an analytical curve with a good linear correlation (R=0.998 which allows one to determine contents of NR and SBR in blends of similar composition.

  6. PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors.

    Directory of Open Access Journals (Sweden)

    Fernando J Bustos

    Full Text Available Considerable evidence indicates that the NMDA receptor (NMDAR subunits NR2A and NR2B are critical mediators of synaptic plasticity and dendritogenesis; however, how they differentially regulate these processes is unclear. Here we investigate the roles of the NR2A and NR2B subunits, and of their scaffolding proteins PSD-95 and SAP102, in remodeling the dendritic architecture of developing hippocampal neurons (2-25 DIV. Analysis of the dendritic architecture and the temporal and spatial expression patterns of the NMDARs and anchoring proteins in immature cultures revealed a strong positive correlation between synaptic expression of the NR2B subunit and dendritogenesis. With maturation, the pruning of dendritic branches was paralleled by a strong reduction in overall and synaptic expression of NR2B, and a significant elevation in synaptic expression of NR2A and PSD95. Using constructs that alter the synaptic composition, we found that either over-expression of NR2B or knock-down of PSD95 by shRNA-PSD95 augmented dendritogenesis in immature neurons. Reactivation of dendritogenesis could also be achieved in mature cultured neurons, but required both manipulations simultaneously, and was accompanied by increased dendritic clustering of NR2B. Our results indicate that the developmental increase in synaptic expression of PSD95 obstructs the synaptic clustering of NR2B-NMDARs, and thereby restricts reactivation of dendritic branching. Experiments with shRNA-PSD95 and chimeric NR2A/NR2B constructs further revealed that C-terminus of the NR2B subunit (tail was sufficient to induce robust dendritic branching in mature hippocampal neurons, and suggest that the NR2B tail is important in recruiting calcium-dependent signaling proteins and scaffolding proteins necessary for dendritogenesis.

  7. 75 FR 2929 - Proposed Collection; Comment Request for Form 13614-NR

    Science.gov (United States)

    2010-01-19

    ... volunteer tax return preparers receive quality training and tools, form 13614-NR ensures they consistently... displays a valid OMB control number. Books or records relating to a collection of information must be.... Generally, tax returns and tax return information are confidential, as required by 26 U.S.C. 6103. Request...

  8. SRC Inhibition Reduces NR2B Surface Expression and Synaptic Plasticity in the Amygdala

    Science.gov (United States)

    Sinai, Laleh; Duffy, Steven; Roder, John C.

    2010-01-01

    The Src protein tyrosine kinase plays a central role in the regulation of N-methyl-d-aspartate receptor (NMDAR) activity by regulating NMDAR subunit 2B (NR2B) surface expression. In the amygdala, NMDA-dependent synaptic plasticity resulting from convergent somatosensory and auditory inputs contributes to emotional memory; however, the role of Src…

  9. WEEKLY BULLETIN PUBLICATION Jeûne genevois - Weekly Bulletin nr 37/2000

    CERN Multimedia

    Section Media & Publications; Tél. 73830; Media & Publication Section; ETT Division; Tel. 73830

    2000-01-01

    As Thursday 7 September is a Holiday (Jeûne genevois), material for publication in Weekly Bulletin nr 37/2000, to appear on Friday 8 September and covering the week of Monday 11 September, should reach us one day earlier than usual, I.E. by noon on Monday 4 September. Thank for your understanding.

  10. Schostakowitsch: Sinfonie Nr. 12 op. 112 (Das Jahr 1917) / Hans-Christian Dadelsen

    Index Scriptorium Estoniae

    Dadelsen, Hans-Christian

    1991-01-01

    Uuest heliplaadist "Schostakowitsch: Sinfonie Nr. 12 op. 112 (Das Jahr 1917). Hamlet-Suite op. 32a. Das goldene Zeitalter [The Age of Gold] (Suite) op. 22a. Göteborger Symphoniker, Neeme Järvi". DG CD 431 688-2 (WD:77'21") DDD

  11. Behavioral assays for evaluating host preferences of Euwallacea nr. fornicatus (Coleoptera: Curculionidae: Scolytinae).

    Science.gov (United States)

    In 2010, the exotic ambrosia beetle, Euwallacea nr. fornicatus Eichhoff (Coleoptera: Curculionidae: Scolytinae) was first discovered in Florida avocado groves. Introduction of its symbiotic Fusarium spp. fungi into galleries in the xylem tissue results in Fusarium-dieback disease. Unlike most ambros...

  12. miR-27b inhibits gastric cancer metastasis by targeting NR2F2

    Directory of Open Access Journals (Sweden)

    Qingzhao Feng

    2016-11-01

    Full Text Available ABSTRACT Increasing attention is focused on the down-regulation of miRNAs in cancer process. Nuclear receptor subfamily 2 (NR2F2, also known as COUP-TFII is involved in the development of many types of cancers, but its role in gastric cancer remains elusive. In this experiment, oncomine and Kaplan-meier database revealed that NR2F2 was up-regulated in gastric cancer and that the high NR2F2 expression contributed to poor survival. MicroRNA-27b was targeted and down-regulated by NR2F2 in human gastric cancer tissues and cells. The ectopic expression of miR-27b inhibited gastric cancer cell proliferation and tumor growth in vitro and in vivo. Assays suggested that the overexpression of miR-27b could promote MGC-803 cells’ migration and invasion and retard their metastasis to the liver. In addition, down-regulation of miR-27b enhanced GES-1 cells’ proliferation and metastasis in vitro. These findings reveal that miR-27b is a tumor suppressor in gastric cancer and a biomarker for improving patients’ survival.

  13. Mahler: Sinfonie Nr. 8 Es-Dur (Sinfonie der Tausend) / Norbert Rüdell

    Index Scriptorium Estoniae

    Rüdell, Norbert

    1995-01-01

    Uuest heliplaadist "Mahler: Sinfonie Nr. 8 Es-Dur (Sinfonie der Tausend). Estonian Boys' Choir, Brunnsbo Children's Choir, Gothenburg Opera Chorus, Royal Stockholm Philharmonic Choir, Gothenburg Symphony Chorus, Gothenburg Opera Chorus, Gothenburg Symphony Orchestra, Neeme Järvi. BIS/Disco-Center CD 700 (WD:72'18")

  14. Barber: Sinfonie Nr. 1, Op. 9, Neeme Järvi / Bernhard Uske

    Index Scriptorium Estoniae

    Uske, Bernhard

    1991-01-01

    Uuest heliplaadist "Barber: Sinfonie Nr. 1, Op. 9. Ouvertüre School for Scandal, Op. 5; Beach: Sinfonie e-Moll, Op. 32, "Gaelic". Detroit Symphony Orchestra /Neeme Järvi". Chandes cassette ABTD 1550; CD CHAN 8958 (72 minutes)

  15. Passage nr. 47: Det encyklopædiske

    DEFF Research Database (Denmark)

    I Passage nr. 47 underkastes det svært bestemmelige encyklopædiske felt en nærmere undersøgelse. Med en række artikler, der gransker det encyklopædiske lige fra senantikkens eklektiske genbrug til informationssamfundets overflod af såvel nyttige som ubrugelige oplysninger, og som griber feltet an...

  16. Development of radiotracers for imaging NR2B subtype NMDA receptors with positron emission tomography

    International Nuclear Information System (INIS)

    Labas, R.

    2007-01-01

    The aim of this thesis was to develop new radioactive tracers for imaging NR2B subtype NMDA receptors with positron emission tomography. Several compounds including 4-(4-fluoro-benzyl)piperidine and presenting interesting in vivo biological properties were the object of a labelling with a positrons emitter atom ( 11 C or 18 F)

  17. Net-Ready Key Performance Parameter (NR-KPP) Implementation Guidebook. Version 2.0

    Science.gov (United States)

    2011-09-30

    Information Enterprise Architecture ( DIEA ) business rules and principles, and DISR policies. DoDAF views produced throughout the NR-KPP development process...of Defense DoDAF Department of Defense Architecture Framework DIEA DoD Information Enterprise Architecture DISA Defense Information Systems Agency

  18. Qualitative risk assessment for the 100-NR-2 Operable Unit. Revision 1

    International Nuclear Information System (INIS)

    1995-03-01

    This qualitative risk assessment provides information about the 100- NR-2 Groundwater Operable Unit of the Hanford reservation. Topics discussed in this report are: data evaluation; human health risk assessment overview; ecological evaluations; summary of uncertainty; results of both the ecological and human health evaluations; toxicity assessment; risk characterization; and a summary of contaminants of potential concern

  19. NR2 subunits and NMDA receptors on lamina II inhibitory and excitatory interneurons of the mouse dorsal horn

    Directory of Open Access Journals (Sweden)

    MacDermott Amy B

    2010-05-01

    Full Text Available Abstract Background NMDA receptors expressed by spinal cord neurons in the superficial dorsal horn are involved in the development of chronic pain associated with inflammation and nerve injury. The superficial dorsal horn has a complex and still poorly understood circuitry that is mainly populated by inhibitory and excitatory interneurons. Little is known about how NMDA receptor subunit composition, and therefore pharmacology and voltage dependence, varies with neuronal cell type. NMDA receptors are typically composed of two NR1 subunits and two of four NR2 subunits, NR2A-2D. We took advantage of the differences in Mg2+ sensitivity of the NMDA receptor subtypes together with subtype preferring antagonists to identify the NR2 subunit composition of NMDA receptors expressed on lamina II inhibitory and excitatory interneurons. To distinguish between excitatory and inhibitory interneurons, we used transgenic mice expressing enhanced green fluorescent protein driven by the GAD67 promoter. Results Analysis of conductance ratio and selective antagonists showed that lamina II GABAergic interneurons express both the NR2A/B containing Mg2+ sensitive receptors and the NR2C/D containing NMDA receptors with less Mg2+ sensitivity. In contrast, excitatory lamina II interneurons express primarily NR2A/B containing receptors. Despite this clear difference in NMDA receptor subunit expression in the two neuronal populations, focally stimulated synaptic input is mediated exclusively by NR2A and 2B containing receptors in both neuronal populations. Conclusions Stronger expression of NMDA receptors with NR2C/D subunits by inhibitory interneurons compared to excitatory interneurons may provide a mechanism to selectively increase activity of inhibitory neurons during intense excitatory drive that can provide inhibitory feedback.

  20. Especificidad del hongo micorrizico (Rhizoctonia sp.) en Phalaenopsis sp., Cymbidium sp., Trichoceros antenifer, Oncidium excavatum, y Cyrtochilum sp.

    OpenAIRE

    Ordoñez, Silvia L.; Pillacela Zhunio, Dora Priscila; Salazar, Jazmín M.; Peña Tapia, Denisse Fabiola

    2016-01-01

    Las orquídeas producen abundantes semillas pequeñas, careciendo de endospermo, cotiledones y sustancias de reserva para llevar a cabo su germinación. Es por esto que estratégicamente las semillas establecen una relación simbiótica con un hongo micorrízico que favorece a su germinación y desarrollo. El objetivo de este estudio fue determinar la especificidad del hongo micorrízico (Rhizoctonia sp.) en la germinación de cinco géneros de orquídeas. Se usaron dos medios de cultivo: 1) PhytamaxTM y...

  1. HCV core protein promotes hepatocyte proliferation and chemoresistance by inhibiting NR4A1

    International Nuclear Information System (INIS)

    Tan, Yongsheng; Li, Yan

    2015-01-01

    This study investigated the effect of HCV core protein on the proliferation of hepatocytes and hepatocellular carcinoma cells (HCC), the influence of HCV core protein on HCC apoptosis induced by the chemotherapeutic agent cisplatin, and the mechanism through which HCV core protein acts as a potential oncoprotein in HCV-related HCC by measuring the levels of NR4A1 and Runt-related transcription factor 3 (RUNX3), which are associated with tumor suppression and chemotherapy resistance. In the present study, PcDNA3.1-core and RUNX3 siRNA were transfected into LO2 and HepG2 cells using Lipofectamine 2000. LO2-core, HepG2-core, LO2-RUNX3 low and control cells were treated with different concentrations of cisplatin for 72 h, and cell proliferation and apoptosis were assayed using the CellTiter 96 ® Aqueous Non-Radioactive Cell Proliferation Assay Kit. Western blot and real time PCR analyses were used to detect NR4A1, RUNX3, smad7, Cyclin D1 and BAX. Confocal microscopy was used to determine the levels of NR4A1 in HepG2 and HepG2-core cells. The growth rate of HepG2-core cells was considerably greater than that of HepG2 cells. HCV core protein increased the expression of cyclin D1 and decreased the expressions of NR4A1 and RUNX3. In LO2 – RUNX3 low , the rate of cell proliferation and the level of cisplatin resistance were the same as in the LO2 -core. These results suggest that HCV core protein decreases the sensitivity of hepatocytes to cisplatin by inhibiting the expression of NR4A1 and promoting the expression of smad7, which negatively regulates the TGF-β pathway. This effect results in down regulation of RUNX3, a target of the TGF-β pathway. Taken together, these findings indicate that in hepatocytes, HCV core protein increases drug resistance and inhibits cell apoptosis by inhibiting the expressions of NR4A1 and RUNX3. - Highlights: • HCV core protein inhibits HepG2 cell sensitivity to cisplatin. • Core expression in HepG2 decreases expression of NR4A1.

  2. HCV core protein promotes hepatocyte proliferation and chemoresistance by inhibiting NR4A1

    Energy Technology Data Exchange (ETDEWEB)

    Tan, Yongsheng, E-mail: yongshengtanwhu@126.com; Li, Yan, E-mail: liyansd2@163.com

    2015-10-23

    This study investigated the effect of HCV core protein on the proliferation of hepatocytes and hepatocellular carcinoma cells (HCC), the influence of HCV core protein on HCC apoptosis induced by the chemotherapeutic agent cisplatin, and the mechanism through which HCV core protein acts as a potential oncoprotein in HCV-related HCC by measuring the levels of NR4A1 and Runt-related transcription factor 3 (RUNX3), which are associated with tumor suppression and chemotherapy resistance. In the present study, PcDNA3.1-core and RUNX3 siRNA were transfected into LO2 and HepG2 cells using Lipofectamine 2000. LO2-core, HepG2-core, LO2-RUNX3 {sup low} and control cells were treated with different concentrations of cisplatin for 72 h, and cell proliferation and apoptosis were assayed using the CellTiter 96{sup ®}Aqueous Non-Radioactive Cell Proliferation Assay Kit. Western blot and real time PCR analyses were used to detect NR4A1, RUNX3, smad7, Cyclin D1 and BAX. Confocal microscopy was used to determine the levels of NR4A1 in HepG2 and HepG2-core cells. The growth rate of HepG2-core cells was considerably greater than that of HepG2 cells. HCV core protein increased the expression of cyclin D1 and decreased the expressions of NR4A1 and RUNX3. In LO2 – RUNX3 {sup low}, the rate of cell proliferation and the level of cisplatin resistance were the same as in the LO2 -core. These results suggest that HCV core protein decreases the sensitivity of hepatocytes to cisplatin by inhibiting the expression of NR4A1 and promoting the expression of smad7, which negatively regulates the TGF-β pathway. This effect results in down regulation of RUNX3, a target of the TGF-β pathway. Taken together, these findings indicate that in hepatocytes, HCV core protein increases drug resistance and inhibits cell apoptosis by inhibiting the expressions of NR4A1 and RUNX3. - Highlights: • HCV core protein inhibits HepG2 cell sensitivity to cisplatin. • Core expression in HepG2 decreases

  3. Rodentibacter gen. nov including Rodentibacter pneumotropicus comb. nov., Rodentibacter heylii sp nov., Rodentibacter myodis sp nov., Rodentibacter ratti sp nov., Rodentibacter heidelbergensis sp nov., Rodentibacter trehalosifermentans sp nov., Rodentibacter rarus sp nov., Rodentibacter mrazii

    DEFF Research Database (Denmark)

    Adhikary, Sadhana; Nicklas, Werner; Bisgaard, Magne

    2017-01-01

    genomic comparison allowed the estimation of DNA–DNA renaturation. Rodentibacter heylii sp. nov. was proposed for a group that included the biovar Heyl of [ Pasteurella ] pneumotropica with the type strain ATCC 12555T (=CCUG 998T). A group was proposed as Rodentibacter ratti sp. nov., which included...

  4. Xylanolytic enzyme systems in Arthrobacter sp MTCC 5214 and Lactobacillus sp.

    Digital Repository Service at National Institute of Oceanography (India)

    Khandeparker, R.; Jalal, T.

    The production of extracellular xylanolytic enzymes such as xylanase, alfa-L-arabinofuranosidase (alfa-l-AFase), and acetyl xylan esterase (Axe) by marine Arthrobacter sp and Lactobacillus sp was investigated using different carbon sources Induction...

  5. Evaluation of antioxidant and cytotoxic properties of Cynobacteria, Limnothrix sp. and Leptolyngbya sp. from Arabian sea

    Digital Repository Service at National Institute of Oceanography (India)

    Anas, A.; Vinothkumar, S.; Gupta, S.; Jasmin, C.; Joseph, V.; Parameswaran, P.S.; Nair, S.

    The hexane fractions of the marine cyanobacteria: Leptolyngbya sp. and Limnothrix sp., collected from Arabian Sea were found to display promising antioxidant properties than their ethyl acetate fraction during radical scavenging ABTS/DPPH assays (IC...

  6. Uso de una conantokina y anticuerpos policlonales para identificar la subunidad NR2B del receptor

    Directory of Open Access Journals (Sweden)

    Leonardo Lareo,†

    2010-12-01

    Full Text Available Objetivo. Proponer una metodología de identificación de la subunidad NR2B, mediante el uso de conantokina G, así como una adecuada extracción de la subunidad NR2B. Materiales y métodos. Se ensayaron dos metodologías para la extracción de la subunidad NR2B de cerebro de rata adulta, la primera buscó la extracción de la subunidad a partir de la membrana mediante la utilización del detergente deoxicolato de sodio y la segunda, garantizó primero la solubilización y eliminación de proteínas citoplasmáticas para luego realizar la extracción de la subunidad mediante el uso del mismo detergente, a partir del pellet generado en la centrifugación del extracto obtenido. Adicionalmente se biotiniló la conantokina G para evaluar su eficiencia en la identificación de la subunidad y comparar los resultados con los obtenidos por metodologías tradicionales como DOT-BLOT, WESTERN-BLOT, ELISA e Inmunohistoquímica. Resultados. La segunda metodología mostró mayor extracción de NR2B por lo que se seleccionó para la realización de los extractos posteriores. Los ensayos de identificación con la conantokina biotinilada evidenciaron interferencia en el reconocimiento, haciéndose necesaria la identificación de la presencia de la subunidad NR2B mediante el uso de anticuerpos policlonales en los ensayos mencionados. Conclusiones. Se propone que hay un impedimento de tipo estérico en el marcaje de la conantokina con la biotina lo que no favorece la interacción de este péptido con la subunidad.

  7. First report of Anisakis sp. in Epinephelus sp. in East Indonesia

    OpenAIRE

    Annytha Ina Rohi Detha; Diana Agustiani Wuri; Julianty Almet; Yuni Riwu; Christin Melky

    2018-01-01

    Objective: The present research was conducted to identify the prevalence of Anisakis sp. as fish-borne zoonoses in Epinephelus sp. in territorial waters of East Nusa Tenggara, Indonesia. Materials and methods: A total of 50 fish (Epinephelus sp.) were collected from Kupang Fish Market in East Nusa Tenggara. Identification of Anisakis sp. was performed based on morphological observations considering shape of ventriculus, boring tooth, and mucron using binocular microscope. Results: Prev...

  8. Stalin Coctail: Schostakowitsch Kammersinfonie Nr. 2 (aus Streichquartett Nr. 3 op. 73). Pärt, Arvo: Collage über das Thema B-A-C-H / Susanne Benda

    Index Scriptorium Estoniae

    Benda, Susanne

    1995-01-01

    Uuest heliplaadist "Stalin Coctail: Schostakowitsch Kammersinfonie Nr. 2 (aus Streichquartett Nr. 3 op. 73). Pärt, Arvo: Collage über das Thema B-A-C-H. Cantus in memoriam B. Britten. Denisov Variationen über Haydns Kanon Tod ist ein langer Schlaf. Schtschedrin Stalin Coctail. Moskauer Virtuosen, Vladimir Spivakov. RCA/BMG-Ariola CD 09026 68061 2 (WD: 67'33")

  9. Vliv hipokampální aplikace Nr1/Nr2 antisense oligodeoxynukleotidů na expresi proteinů postsynaptické denzity a na prepulzní inhibici

    Czech Academy of Sciences Publication Activity Database

    Vrajová, M.; Klaschka, Jan; Tejkalová, H.; Bubeníková-Valešová, V.

    2011-01-01

    Roč. 15, Suppl. 2 (2011), s. 11-14 ISSN 1211-7579 R&D Projects: GA MŠk(CZ) 1M0517 Institutional research plan: CEZ:AV0Z10300504 Keywords : NMDA receptor * PSD proteins * antisense oligodeoxynucleotides for NMDA-NR1/NR2 subunits * prepulse inhibition Subject RIV: FL - Psychiatry, Sexuology http://www.tigis.cz/images/stories/psychiatrie/2011/s2/03_vrajova_cns_2-11.pdf

  10. Key Amino Acid Residues within the Third Membrane Domains of NR1 and NR2 Subunits Contribute to the Regulation of the Surface Delivery of N-methyl-D-aspartate Receptors

    Czech Academy of Sciences Publication Activity Database

    Kaniaková, Martina; Krausová, Barbora; Vyklický, Vojtěch; Kořínek, Miloslav; Lichnerová, Katarina; Vyklický ml., Ladislav; Horák, Martin

    2012-01-01

    Roč. 287, č. 31 (2012), s. 26423-26434 ISSN 0021-9258 R&D Projects: GA ČR(CZ) GAP303/11/0075; GA ČR(CZ) GBP304/12/G069; GA ČR(CZ) GAP303/12/1464 Institutional research plan: CEZ:AV0Z50110509 Institutional support: RVO:67985823 Keywords : NMDA receptor * NR1 * NR2 Subject RIV: ED - Physiology Impact factor: 4.651, year: 2012

  11. Water Data Analysis 393937093090901 TURKEY CREEK NR SUMNER MO, FULBRIGHT RD NEAR SWAN LAKE NWR-2013-2016

    Data.gov (United States)

    Department of the Interior — WATER MONITORING STATION ANALYSIS – CALENDAR YEAR 2013 to 2016 SITE NUMBER: 393937093090901 SITE NAME: Turkey Creek nr Sumner MO, Fulbright Rd COOPERATION: Swan Lake...

  12. Specialised predation by Palpimanus sp. (Araneae: Palpimanidae ...

    African Journals Online (AJOL)

    This is the first detailed report on the natural prey and the prey-capture tactics of a Palpimanus sp. from Entebbe (Uganda). Although this species fed occasionally on insects, its dominant prey in the field was other spiders, especially jumping spiders (Salticidae) and their eggs. Encounters between Palpimanus sp. and ...

  13. Wide spectrum of NR5A1‐related phenotypes in 46,XY and 46,XX individuals

    Science.gov (United States)

    Domenice, Sorahia; Machado, Aline Zamboni; Ferreira, Frederico Moraes; Ferraz‐de‐Souza, Bruno; Lerario, Antonio Marcondes; Lin, Lin; Nishi, Mirian Yumie; Gomes, Nathalia Lisboa; da Silva, Thatiana Evelin; Silva, Rosana Barbosa; Correa, Rafaela Vieira; Montenegro, Luciana Ribeiro; Narciso, Amanda; Costa, Elaine Maria Frade; Achermann, John C

    2016-01-01

    Steroidogenic factor 1 (NR5A1, SF‐1, Ad4BP) is a transcriptional regulator of genes involved in adrenal and gonadal development and function. Mutations in NR5A1 have been among the most frequently identified genetic causes of gonadal development disorders and are associated with a wide phenotypic spectrum. In 46,XY individuals, NR5A1‐related phenotypes may range from disorders of sex development (DSD) to oligo/azoospermia, and in 46,XX individuals, from 46,XX ovotesticular and testicular DSD to primary ovarian insufficiency (POI). The most common 46,XY phenotype is atypical or female external genitalia with clitoromegaly, palpable gonads, and absence of Müllerian derivatives. Notably, an undervirilized external genitalia is frequently seen at birth, while spontaneous virilization may occur later, at puberty. In 46,XX individuals, NR5A1 mutations are a rare genetic cause of POI, manifesting as primary or secondary amenorrhea, infertility, hypoestrogenism, and elevated gonadotropin levels. Mothers and sisters of 46,XY DSD patients carrying heterozygous NR5A1 mutations may develop POI, and therefore require appropriate counseling. Moreover, the recurrent heterozygous p.Arg92Trp NR5A1 mutation is associated with variable degrees of testis development in 46,XX patients. A clear genotype‐phenotype correlation is not seen in patients bearing NR5A1 mutations, suggesting that genetic modifiers, such as pathogenic variants in other testis/ovarian‐determining genes, may contribute to the phenotypic expression. Here, we review the published literature on NR5A1‐related disease, and discuss our findings at a single tertiary center in Brazil, including ten novel NR5A1 mutations identified in 46,XY DSD patients. The ever‐expanding phenotypic range associated with NR5A1 variants in XY and XX individuals confirms its pivotal role in reproductive biology, and should alert clinicians to the possibility of NR5A1 defects in a variety of phenotypes presenting with gonadal

  14. Guide til gode spørgeskemaer

    DEFF Research Database (Denmark)

    Olsen, Henning

    Spørgeskemaundersøgelser bliver ofte brugt til at dokumentere forskellige forhold og begrunde politiske beslutninger. Men resultaterne kan være forbundet med stor usikkerhed. Det kræver omhu og stor sproglig formåen at udarbejde spørgeskemaer. Seniorforsker Henning Olsen har i flere år arbejdet med...... viden om, hvordan folk forstår sproglige meddelelser og genkalder sig informationer. I guiden behandles emner som fx styrende problemstillinger og spørgsmåls fokus og neutralitet, formulering af åbne eller lukkede spørgsmål og svarkategorier, tematiske spørgeforløb, aflastning af svarpersoners...

  15. Bovine NR1I3 gene polymorphisms and its association with feed efficiency traits in Nellore cattle

    Science.gov (United States)

    Alexandre, Pâmela A.; Gomes, Rodrigo C.; Santana, Miguel H.A.; Silva, Saulo L.; Leme, Paulo R.; Mudadu, Maurício A.; Regitano, Luciana C.A.; Meirelles, Flávio V.; Ferraz, José B.S.; Fukumasu, Heidge

    2014-01-01

    Abstract The Nuclear receptor 1 family I member 3 (NR1I3), also known as the Constitutive Androstane Receptor (CAR), was initially characterized as a key regulator of xenobiotic metabolism. However, recent biochemical and structural data suggest that NR1I3 is activated in response to metabolic and nutritional stress in a ligand-independent manner. Thus, we prospected the Bovine NR1I3 gene for polymorphisms and studied their association with feed efficiency traits in Nellore cattle. First, 155 purebred Nellore bulls were individually measured for Residual Feed Intake (RFI) and the 25 best (High Feed Efficiency group, HFE) and the 25 worst animals (Low Feed Efficiency group, LFE) were selected for DNA extraction. The entire Bovine NR1I3 gene was amplified and polymorphisms were identified by sequencing. Then, one SNP different between HFE and LFE groups was genotyped in all the 155 animals and in another 288 animals totalizing 443 Nellore bulls genotyped for association of NR1I3 SNPs with feed efficiency traits. We found 24 SNPs in the NR1I3 gene and choose a statistically different SNP between HFE and LFE groups for further analysis. Genotyping of the 155 animals showed a significant association within SNP and RFI (p = 0.04), Residual Intake and BW Gain (p = 0.04) and Dry Matter Intake (p = 0.01). This SNP is located in the 5′flanking promoter region of NR1I3 gene and different alleles alter the binding site for predicted transcriptional factors as HNF4alpha, CREM and c-MYB, leading us to conclude that NR1I3 expression and regulation might be important to feed efficiency. PMID:25606404

  16. Activation of nuclear receptor NR5A2 increases Glut4 expression and glucose metabolism in muscle cells

    Energy Technology Data Exchange (ETDEWEB)

    Bolado-Carrancio, A. [Department of Molecular Biology, University of Cantabria, IDIVAL, Santander (Spain); Riancho, J.A. [Department of Internal Medicine, Hospital U.M. Valdecilla-IDIVAL, University of Cantabria, RETICEF, Santander (Spain); Sainz, J. [Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC), CSIC-University of Cantabria, Santander (Spain); Rodríguez-Rey, J.C., E-mail: rodriguj@unican.es [Department of Molecular Biology, University of Cantabria, IDIVAL, Santander (Spain)

    2014-04-04

    Highlights: • NR5A2 expression in C2C12 is associated with myotube differentiation. • DLPC induces an increase in GLUT4 levels and glucose uptake in C2C12 myotubes. • In high glucose conditions the activation of NR5A2 inhibits fatty acids oxidation. - Abstract: NR5A2 is a nuclear receptor which regulates the expression of genes involved in cholesterol metabolism, pluripotency maintenance and cell differentiation. It has been recently shown that DLPC, a NR5A2 ligand, prevents liver steatosis and improves insulin sensitivity in mouse models of insulin resistance, an effect that has been associated with changes in glucose and fatty acids metabolism in liver. Because skeletal muscle is a major tissue in clearing glucose from blood, we studied the effect of the activation of NR5A2 on muscle metabolism by using cultures of C2C12, a mouse-derived cell line widely used as a model of skeletal muscle. Treatment of C2C12 with DLPC resulted in increased levels of expression of GLUT4 and also of several genes related to glycolysis and glycogen metabolism. These changes were accompanied by an increased glucose uptake. In addition, the activation of NR5A2 produced a reduction in the oxidation of fatty acids, an effect which disappeared in low-glucose conditions. Our results suggest that NR5A2, mostly by enhancing glucose uptake, switches muscle cells into a state of glucose preference. The increased use of glucose by muscle might constitute another mechanism by which NR5A2 improves blood glucose levels and restores insulin sensitivity.

  17. Predictive value of Sp1/Sp3/FLIP signature for prostate cancer recurrence.

    Directory of Open Access Journals (Sweden)

    Roble G Bedolla

    Full Text Available Prediction of prostate cancer prognosis is challenging and predictive biomarkers of recurrence remain elusive. Although prostate specific antigen (PSA has high sensitivity (90% at a PSA level of 4.0 ng/mL, its low specificity leads to many false positive results and considerable overtreatment of patients and its performance at lower ranges is poor. Given the histopathological and molecular heterogeneity of prostate cancer, we propose that a panel of markers will be a better tool than a single marker. We tested a panel of markers composed of the anti-apoptotic protein FLIP and its transcriptional regulators Sp1 and Sp3 using prostate tissues from 64 patients with recurrent and non-recurrent cancer who underwent radical prostatectomy as primary treatment for prostate cancer and were followed with PSA measurements for at least 5 years. Immunohistochemical staining for Sp1, Sp3, and FLIP was performed on these tissues and scored based on the proportion and intensity of staining. The predictive value of the FLIP/Sp1/Sp3 signature for clinical outcome (recurrence vs. non-recurrence was explored with logistic regression, and combinations of FLIP/Sp1/Sp3 and Gleason score were analyzed with a stepwise (backward and forward logistic model. The discrimination of the markers was identified by sensitivity-specificity analysis and the diagnostic value of FLIP/Sp1/Sp3 was determined using area under the curve (AUC for receiver operator characteristic curves. The AUCs for FLIP, Sp1, Sp3, and Gleason score for predicting PSA failure and non-failure were 0.71, 0.66, 0.68, and 0.76, respectively. However, this increased to 0.93 when combined. Thus, the "biomarker signature" of FLIP/Sp1/Sp3 combined with Gleason score predicted disease recurrence and stratified patients who are likely to benefit from more aggressive treatment.

  18. Preliminary study of semi-refined carrageenan (SRC) as secondary gelling agent in natural rubber (NR) latex foam

    Science.gov (United States)

    Norhazariah, S.; Azura, A. R.; Azahari, B.; Sivakumar, R.

    2017-12-01

    Semi-refined carrageenan (SRC) product is considerably cheaper and easier to produce as a natural polysaccharide, which was utilized in food and other product application. However, the application in latex is limited. The aim of this work is to evaluate the SRC produced from low industrial grade seaweed (LIGS) in the latex foam application. The FTIR spectra showed the SRC produced as kappa type carrageenan with lower sulfur content compared to native LIGS. NR latex foam is produced by using the Dunlop method with some modifications. The effect of SRC loading as a secondary gelling agent in NR latex foam is investigated. The density and morphology of the NR latex foam with the addition of the SRC are analyzed. NR latex foam density increased with SRC loading and peaked at 1.8 phr SRC. The addition of SRC has induced the bigger cell size compared to the cell size of the control NR latex foam, as shown in the optical micrograph. It can be concluded that SRC LIGS could be acted as secondary gelling agent in NR latex foam.

  19. The NMDA Receptor NR1 C1 Region Bound to Calmodulin: Structural Insights into Functional Differences between Homologous Domains

    Energy Technology Data Exchange (ETDEWEB)

    Ataman, Zeynep Akyol; Gakhar, Lokesh; Sorensen, Brenda R.; Hell, Johannes W.; Shea, Madeline A. (Iowa)

    2008-09-17

    Calmodulin (CaM) regulates tetrameric N-methyl-D-aspartate receptors (NMDARs) by binding tightly to the C0 and C1 regions of its NR1 subunit. A crystal structure (2HQW; 1.96 {angstrom}) of calcium-saturated CaM bound to NR1C1 (peptide spanning 875-898) showed that NR1 S890, whose phosphorylation regulates membrane localization, was solvent protected, whereas the endoplasmic reticulum retention motif was solvent exposed. NR1 F880 filled the CaM C-domain pocket, whereas T886 was closest to the N-domain pocket. This 1-7 pattern was most similar to that in the CaM-MARCKS complex. Comparison of CaM-ligand wrap-around conformations identified a core tetrad of CaM C-domain residues (FLMM{sub C}) that contacted all ligands consistently. An identical tetrad of N-domain residues (FLMM{sub N}) made variable sets of contacts with ligands. This CaM-NR1C1 structure provides a foundation for designing mutants to test the role of CaM in NR1 trafficking as well as insights into how the homologous CaM domains have different roles in molecular recognition.

  20. Enzyme kinetics, inhibitors, mutagenesis and electron paramagnetic resonance analysis of dual-affinity nitrate reductase in unicellular N(2)-fixing cyanobacterium Cyanothece sp. PCC 8801.

    Science.gov (United States)

    Wang, Tung-Hei; Chen, Yung-Han; Huang, Jine-Yung; Liu, Kang-Cheng; Ke, Shyue-Chu; Chu, Hsiu-An

    2011-11-01

    The assimilatory nitrate reductase (NarB) of N(2)-fixing cyanobacterium Cyanothece sp. PCC 8801 is a monomeric enzyme with dual affinity for substrate nitrate. We purified the recombinant NarB of Cyanothece sp. PCC 8801 and further investigated it by enzyme kinetics analysis, site-directed mutagenesis, inhibitor kinetics analysis, and electron paramagnetic resonance (EPR) spectroscopy. The NarB showed 2 kinetic regimes at pH 10.5 or 8 and electron-donor conditions methyl viologen or ferredoxin (Fd). Fd-dependent NR assay revealed NarB with very high affinity for nitrate (K(m)1, ∼1μM; K(m)2, ∼270μM). Metal analysis and EPR results showed that NarB contains a Mo cofactor and a [4Fe-4S] cluster. In addition, the R352A mutation on the proposed nitrate-binding site of NarB greatly altered both high- and low-affinity kinetic components. Furthermore, the effect of azide on the NarB of Cyanothece sp. PCC 8801 was more complex than that on the NarB of Synechococcus sp. PCC 7942 with its single kinetic regime. With 1mM azide, the kinetics of the wild-type NarB was transformed from 2 kinetic regimes to hyperbolic kinetics, and its activity was enhanced significantly under medium nitrate concentrations. Moreover, EPR results also suggested a structural difference between the two NarBs. Taken together, our results show that the NarB of Cyanothece sp. PCC 8801 contains only a single Mo-catalytic center, and we rule out that the enzyme has 2 independent, distinct catalytic sites. In addition, the NarB of Cyanothece sp. PCC 8801 may have a regulatory nitrate-binding site. Copyright © 2011 Elsevier Masson SAS. All rights reserved.

  1. NAD+Intermediates: The Biology and Therapeutic Potential of NMN and NR.

    Science.gov (United States)

    Yoshino, Jun; Baur, Joseph A; Imai, Shin-Ichiro

    2017-12-14

    Research on the biology of NAD + has been gaining momentum, providing many critical insights into the pathogenesis of age-associated functional decline and diseases. In particular, two key NAD + intermediates, nicotinamide riboside (NR) and nicotinamide mononucleotide (NMN), have been extensively studied over the past several years. Supplementing these NAD + intermediates has shown preventive and therapeutic effects, ameliorating age-associated pathophysiologies and disease conditions. Although the pharmacokinetics and metabolic fates of NMN and NR are still under intensive investigation, these NAD + intermediates can exhibit distinct behavior, and their fates appear to depend on the tissue distribution and expression levels of NAD + biosynthetic enzymes, nucleotidases, and presumptive transporters for each. A comprehensive concept that connects NAD + metabolism to the control of aging and longevity in mammals has been proposed, and the stage is now set to test whether these exciting preclinical results can be translated to improve human health. Copyright © 2017 Elsevier Inc. All rights reserved.

  2. NR4A orphan nuclear receptors in glucose homeostasis: a minireview.

    Science.gov (United States)

    Close, A F; Rouillard, C; Buteau, J

    2013-12-01

    Type 2 diabetes mellitus is a disorder characterized by insulin resistance and a relative deficit in insulin secretion, both of which result in elevated blood glucose. Understanding the molecular mechanisms underlying the pathophysiology of diabetes could lead to the development of new therapeutic approaches. An ever-growing body of evidence suggests that members of the NR4A family of nuclear receptors could play a pivotal role in glucose homeostasis. This review aims to present and discuss advances so far in the evaluation of the potential role of NR4A in the regulation of glucose homeostasis and the development of type 2 diabetes. Copyright © 2013 Elsevier Masson SAS. All rights reserved.

  3. An open-label, non-randomized study of the pharmacokinetics of the nutritional supplement nicotinamide riboside (NR and its effects on blood NAD+ levels in healthy volunteers.

    Directory of Open Access Journals (Sweden)

    Sophia E Airhart

    Full Text Available The co-primary objectives of this study were to determine the human pharmacokinetics (PK of oral NR and the effect of NR on whole blood nicotinamide adenine dinucleotide (NAD+ levels.Though mitochondrial dysfunction plays a critical role in the development and progression of heart failure, no mitochondria-targeted therapies have been translated into clinical practice. Recent murine studies have reported associations between imbalances in the NADH/NAD+ ratio with mitochondrial dysfunction in multiple tissues, including myocardium. Moreover, an NAD+ precursor, nicotinamide mononucleotide, improved cardiac function, while another NAD+ precursor, nicotinamide riboside (NR, improved mitochondrial function in muscle, liver and brown adipose. Thus, PK studies of NR in humans is critical for future clinical trials.In this non-randomized, open-label PK study of 8 healthy volunteers, 250 mg NR was orally administered on Days 1 and 2, then uptitrated to peak dose of 1000 mg twice daily on Days 7 and 8. On the morning of Day 9, subjects completed a 24-hour PK study after receiving 1000 mg NR at t = 0. Whole-blood levels of NR, clinical blood chemistry, and NAD+ levels were analyzed.Oral NR was well tolerated with no adverse events. Significant increases comparing baseline to mean concentrations at steady state (Cave,ss were observed for both NR (p = 0.03 and NAD+ (p = 0.001; the latter increased by 100%. Absolute changes from baseline to Day 9 in NR and NAD+ levels correlated highly (R2 = 0.72, p = 0.008.Because NR increases circulating NAD+ in humans, NR may have potential as a therapy in patients with mitochondrial dysfunction due to genetic and/or acquired diseases.

  4. An open-label, non-randomized study of the pharmacokinetics of the nutritional supplement nicotinamide riboside (NR) and its effects on blood NAD+ levels in healthy volunteers.

    Science.gov (United States)

    Airhart, Sophia E; Shireman, Laura M; Risler, Linda J; Anderson, Gail D; Nagana Gowda, G A; Raftery, Daniel; Tian, Rong; Shen, Danny D; O'Brien, Kevin D

    2017-01-01

    The co-primary objectives of this study were to determine the human pharmacokinetics (PK) of oral NR and the effect of NR on whole blood nicotinamide adenine dinucleotide (NAD+) levels. Though mitochondrial dysfunction plays a critical role in the development and progression of heart failure, no mitochondria-targeted therapies have been translated into clinical practice. Recent murine studies have reported associations between imbalances in the NADH/NAD+ ratio with mitochondrial dysfunction in multiple tissues, including myocardium. Moreover, an NAD+ precursor, nicotinamide mononucleotide, improved cardiac function, while another NAD+ precursor, nicotinamide riboside (NR), improved mitochondrial function in muscle, liver and brown adipose. Thus, PK studies of NR in humans is critical for future clinical trials. In this non-randomized, open-label PK study of 8 healthy volunteers, 250 mg NR was orally administered on Days 1 and 2, then uptitrated to peak dose of 1000 mg twice daily on Days 7 and 8. On the morning of Day 9, subjects completed a 24-hour PK study after receiving 1000 mg NR at t = 0. Whole-blood levels of NR, clinical blood chemistry, and NAD+ levels were analyzed. Oral NR was well tolerated with no adverse events. Significant increases comparing baseline to mean concentrations at steady state (Cave,ss) were observed for both NR (p = 0.03) and NAD+ (p = 0.001); the latter increased by 100%. Absolute changes from baseline to Day 9 in NR and NAD+ levels correlated highly (R2 = 0.72, p = 0.008). Because NR increases circulating NAD+ in humans, NR may have potential as a therapy in patients with mitochondrial dysfunction due to genetic and/or acquired diseases.

  5. Antibacterial Actions and Potential Phototoxic Effects of Volatile oils of Foeniculum sp. (fennel, Salvia sp. (sage, Vitis sp. (grape, Lavandula sp. (lavender

    Directory of Open Access Journals (Sweden)

    Elif Ayse Erdogan Eliuz

    2016-09-01

    Full Text Available In the present study, the volatile compounds of essential oil of Foeniculum vulgare (fennel, Salvia officinalis (sage, Vitis vinifera (grape, Lavandula angustifolia (lavender were analysed by gas chromatography-mass spectrometry (GC-MS using the Nist and Willey libraries. It was determined that the main components of Foeniculum sp. were anethole (41.11%, carvacrol (9.18%. whereas main components of Salvia sp were 1.8 cineole (34.09%, caryophyllene (10.95%, camphor (9.44%, α-pinene (8.42%. Vitis sp. contained linoleic acid (36.98%, 2,4-decadienal (30.79%. Finally, volatile component of Lavandula sp. was linalool (33.57%, linalyl acetate (30.74%. Photoxic antibacterial activity of volatile oil of those plants against Escherichia coli (ATCC 25293, Klebsiella pneumoniae (10031, Salmonella thyphimurium, Bacillus subtilis (ATCC 6633, Staphylococcus aureus (ATCC 25925, Enterococcus feacalis (ATCC 29212 were examined by using disc diffusion method. We demonstrated that volatile oil effectively can be activated by a standard LED light. In vitro, significant phototoxicity was demonstrated by volatile oil of Foeniculum sp. and Vitis sp. (P < 0.05, while minor phototoxicity was induced by Lavandula sp. Therefore, volatile oil of plant can be considered as a potential photosensitizer in the photochemical therapy.

  6. Homology modeling of NR2B modulatory domain of NMDA receptor and analysis of ifenprodil binding.

    Science.gov (United States)

    Marinelli, Luciana; Cosconati, Sandro; Steinbrecher, Thomas; Limongelli, Vittorio; Bertamino, Alessia; Novellino, Ettore; Case, David A

    2007-10-01

    NMDA receptors are glutamate-gated ion channels (iGluRs) that are involved in several important physiological functions such as neuronal development, synaptic plasticity, learning, and memory. Among iGluRs, NMDA receptors have been perhaps the most actively investigated for their role in chronic neurodegeneration such as Alzheimer's, Parkinson's, and Huntington's diseases. Recent studies have shown that the NTD of subunit NR2B modulates ion channel gating through the binding of allosteric modulators such as the prototypical compound ifenprodil. In the present paper, the construction of a three-dimensional model for the NR2B modulatory domain is described and docking calculations allow, for the first time, definition of the ifenprodil binding pose at an atomic level and fully explain all the available structure-activity relationships. Moreover, in an attempt to add further insight into the ifenprodil mechanism of action, as it is not completely clear if it binds and stabilizes an open or a closed conformation of the NR2B modulatory domain, a matter, which is fundamental for the rational design of NMDA antagonists, MD simulations followed by an MM-PBSA analysis were performed. These calculations reveal that the closed conformation of the R1-R2 domain, rather than the open, constitutes the high affinity binding site for ifenprodil and that a profound stabilization of the closed conformation upon ifenprodil binding occurs. Thus, for a rational design and/or for virtual screening experiments, the closed conformation of the R1-R2 domain should be taken into account and our 3D model can provide valuable hints for the design of NR2B-selective antagonists.

  7. TurboSP and the Topological Trigger

    CERN Document Server

    Belavin, Vladislav

    2016-01-01

    TurboSP was originally proposed as an alternative to Full stream in LHCb data flow. TurboSP is a data flow strategy which not only selects events that should be preserved, like in Full stream, but also provides selective persistence. This is achieved by saving candidates and subset of the reconstruction. During this summer project we investigated the physics viability of using TurboSP with the topological lines and found out a possibility to reduce the number of kept tracks per event by two times while keeping a ratio of fully picked up interesting decay modes on $\\sim 97 \\%$ level.

  8. The network and properties of the NR/SBR vulcanizate modified by electron beam irradiation

    Science.gov (United States)

    Shen, Jing; Wen, Shipeng; Du, Yishi; Li, Ning; Zhang, Liqun; Yang, Yusheng; Liu, Li

    2013-11-01

    A natural rubber/styrene butadiene rubber (NR/SBR) vulcanizate filled with carbon black was modified by high-energy electron beam (EB) irradiation in this work. The crosslinked structure was studied by a special chemical probe method. The influence of EB irradiation on mechanical properties, filler network, and dynamic properties including abrasion resistance, rolling resistance, and wet skid resistance was also investigated. The results revealed that the crosslink structure significantly changed after EB treatment, indicating that the amount of poly- and di-sulfide crosslinked bonds decreased and that of mono-sulfide bonds increased. The polymer-filler interaction was enhanced after EB irradiation. An EB dose of 600 kGy reduced the abrasion loss of the NR/SBR vulcanizate, and one of 300 kGy reduced the rolling resistance by 11.4%. Meanwhile, EB doses below 200 kGy had no obvious effect on the wet skid resistance. This EB-modified NR/SBR vulcanizate can be used to prepare high-performance tires with good abrasion resistance and low rolling resistance.

  9. Expression of NR1I3 in mouse lung tumors induced by the tobacco-specific nitrosamine 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone

    Energy Technology Data Exchange (ETDEWEB)

    Fukumasu, H.; Cordeiro, Y.G.; Rochetti, A.L.; Barra, C.N.; Sámora, T.S.; Strefezzi, R.F. [Laboratório de Oncologia Comparada e Translacional, Departmento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP (Brazil); Dagli, M.L.Z. [Laboratório de Oncologia Experimental e Comparada, Departmento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP (Brazil)

    2015-02-13

    Nuclear receptor subfamily 1, group I, member 3 (NR1I3) is reported to be a possible novel therapeutic target for some cancers, including lung, brain and hematopoietic tumors. Here, we characterized expression of NR1I3 in a mouse model of lung carcinogenesis induced by 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone (NNK), the most potent tobacco carcinogen. Lung tumors were collected from mice treated with NNK (400 mg/kg) and euthanized after 52 weeks. Benign and malignant lesions were formalin-fixed and paraffin-embedded for histology and immunohistochemistry, with samples snap-frozen for mRNA analysis. Immunohistochemically, we found that most macrophages and type I and II pneumocytes expressed NR1I3, whereas fibroblasts and endothelial cells were NR1I3{sup −}. Compared with benign lesions, malignant lesions had less NR1I3{sup +} tumor cells. Gene expression analysis also showed an inverse correlation between NR1I3 mRNA expression and tumor size (P=0.0061), suggesting that bigger tumors expressed less NR1I3 transcripts, in accordance with our immunohistochemical NR1I3 tests. Our results indicate that NR1I3 expression decreased during progression of malignant lung tumors induced by NNK in mice.

  10. Epigenetic silencing of the NR4A3 tumor suppressor, by aberrant JAK/STAT signaling, predicts prognosis in gastric cancer

    Science.gov (United States)

    Yeh, Chung-Min; Chang, Liang-Yu; Lin, Shu-Hui; Chou, Jian-Liang; Hsieh, Hsiao-Yen; Zeng, Li-Han; Chuang, Sheng-Yu; Wang, Hsiao-Wen; Dittner, Claudia; Lin, Cheng-Yu; Lin, Jora M. J.; Huang, Yao-Ting; Ng, Enders K. W.; Cheng, Alfred S. L.; Wu, Shu-Fen; Lin, Jiayuh; Yeh, Kun-Tu; Chan, Michael W. Y.

    2016-08-01

    While aberrant JAK/STAT signaling is crucial to the development of gastric cancer (GC), its effects on epigenetic alterations of its transcriptional targets remains unclear. In this study, by expression microarrays coupled with bioinformatic analyses, we identified a putative STAT3 target gene, NR4A3 that was downregulated in MKN28 GC daughter cells overexpressing a constitutively activated STAT3 mutant (S16), as compared to an empty vector control (C9). Bisulphite pyrosequencing and demethylation treatment showed that NR4A3 was epigenetically silenced by promoter DNA methylation in S16 and other GC cell lines including AGS cells, showing constitutive activation of STAT3. Subsequent experiments revealed that NR4A3 promoter binding by STAT3 might repress its transcription. Long-term depletion of STAT3 derepressed NR4A3 expression, by promoter demethylation, in AGS GC cells. NR4A3 re-expression in GC cell lines sensitized the cells to cisplatin, and inhibited tumor growth in vitro and in vivo, in an animal model. Clinically, GC patients with high NR4A3 methylation, or lower NR4A3 protein expression, had significantly shorter overall survival. Intriguingly, STAT3 activation significantly associated only with NR4A3 methylation in low-stage patient samples. Taken together, aberrant JAK/STAT3 signaling epigenetically silences a potential tumor suppressor, NR4A3, in gastric cancer, plausibly representing a reliable biomarker for gastric cancer prognosis.

  11. nr0b1 (DAX1) mutation in zebrafish causes female-to-male sex reversal through abnormal gonadal proliferation and differentiation.

    Science.gov (United States)

    Chen, Sijie; Zhang, Hefei; Wang, Fenghua; Zhang, Wei; Peng, Gang

    2016-09-15

    Sex determinations are diverse in vertebrates. Although many sex-determining genes and pathways are conserved, the mechanistic roles of these genes and pathways in the genetic sex determination are not well understood. DAX1 (encoded by the NR0B1 gene) is a vertebrate specific orphan nuclear receptor that regulates gonadal development and sexual determination. In human, duplication of the NR0B1 gene leads to male-to-female sex reversal. In mice, Nr0b1 shows both pro-testis and anti-testis functions. We generated inheritable nr0b1 mutation in the zebrafish and found the nr0b1 mutation caused homozygous mutants to develop as fertile males due to female-to-male sex reversal. The nr0b1 mutation did not increase Caspase-3 labeling nor tp53 expression in the developing gonads. Introduction of a tp53 mutation into the nr0b1 mutant did not rescue the sex-reversal phenotype. Further examination revealed reduction in cell proliferation and abnormal somatic cell differentiation in the nr0b1 mutant gonads at the undifferentiated and bi-potential ovary stages. Together, our results suggest nr0b1 regulates somatic cell differentiation and cell proliferation to ensure normal sex development in the zebrafish. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  12. Optimalization activity of ZnO NR/TiO2 NR-P3HT as an active layer based on hybrid bulk heterojunction on dye sensitized solar cell (DSSC)

    International Nuclear Information System (INIS)

    Saputri, Liya Nikmatul Maula Zulfa; Ramelan, Ari Handono; Hanif, Qonita Awliya; Hasanah, Yesi Ihdina Fityatal; Prajanira, Lau Bekti; Wahyuningsih, Sayekti

    2016-01-01

    Dye sensitized solar cell (DSSC) with metal inorganic and conjugated organic polymer mixture, ZnO NR/TiO 2 NR-P3HT as an active layer based on hybrid bulk heterojunction has been studied. The hybrid material was used to optimize DSSC performs for better efficiency than only TiO 2 as an electrode. Synthesis of TiO 2 nanorods (NR) was conducted by ball milling 1000 rpm for 4 hours and strong base reaction by hydrothermal process at 120 °C overnight. And the ZnO NR was synthesized from Zn(NO 3 ) 2 .4H 2 O precusor by hydrotermal process at 90 °C for 5 hours and calcined on various temperature s of 400, 600, and 800 °C. ZnO NR was coated into an Tndium Tin Oxide (TTO) glass to collecting electron s effectively, where TiO 2 NR were incorporated with poly(3 -hexylthiophene) (P3HT) on various concentration s of 5, 10, 15 mg/mL to obtain a larger surface area. Material characterization were performed by X -Ray Diffraction (XRD) and Uv-Vis spectrophotometer. For an application of DSSC were measured by T-V Keithley Multimeter and the efficiency of DSSC at various P3HT’s concentrations of 5, 10, 15 mg/mL were 7.44 × 10 −3 , 0.0114, 0.0104, respectively. The maximum efficiency of DSSC was showed when TiO 2 NR-P3HT’s concentration was 10 mg/mL.

  13. Optimalization activity of ZnO NR/TiO2 NR-P3HT as an active layer based on hybrid bulk heterojunction on dye sensitized solar cell (DSSC)

    Energy Technology Data Exchange (ETDEWEB)

    Saputri, Liya Nikmatul Maula Zulfa; Ramelan, Ari Handono; Hanif, Qonita Awliya; Hasanah, Yesi Ihdina Fityatal; Prajanira, Lau Bekti; Wahyuningsih, Sayekti, E-mail: sayektiw@mipa.uns.ac.id [Chemistry Department, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Ir.Sutami 36A Kentingan Surakarta 57/26, Central Java (Indonesia)

    2016-04-19

    Dye sensitized solar cell (DSSC) with metal inorganic and conjugated organic polymer mixture, ZnO NR/TiO{sub 2} NR-P3HT as an active layer based on hybrid bulk heterojunction has been studied. The hybrid material was used to optimize DSSC performs for better efficiency than only TiO{sub 2} as an electrode. Synthesis of TiO{sub 2} nanorods (NR) was conducted by ball milling 1000 rpm for 4 hours and strong base reaction by hydrothermal process at 120 °C overnight. And the ZnO NR was synthesized from Zn(NO{sub 3}){sub 2}.4H{sub 2}O precusor by hydrotermal process at 90 °C for 5 hours and calcined on various temperature s of 400, 600, and 800 °C. ZnO NR was coated into an Tndium Tin Oxide (TTO) glass to collecting electron s effectively, where TiO{sup 2} NR were incorporated with poly(3 -hexylthiophene) (P3HT) on various concentration s of 5, 10, 15 mg/mL to obtain a larger surface area. Material characterization were performed by X -Ray Diffraction (XRD) and Uv-Vis spectrophotometer. For an application of DSSC were measured by T-V Keithley Multimeter and the efficiency of DSSC at various P3HT’s concentrations of 5, 10, 15 mg/mL were 7.44 × 10{sup −3}, 0.0114, 0.0104, respectively. The maximum efficiency of DSSC was showed when TiO{sup 2} NR-P3HT’s concentration was 10 mg/mL.

  14. NCBI nr-aa BLAST: CBRC-TTRU-01-0654 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0654 ref|ZP_05544813.1| multidrug efflux pump BexA [Parabacteroides sp.... D13] ref|ZP_06077587.1| multidrug efflux pump BexA [Bacteroides sp. 2_1_33B] gb|EEU51559.1| multidrug efflux pump... BexA [Parabacteroides sp. D13] gb|EEY81680.1| multidrug efflux pump BexA [Bacteroides sp. 2_1_33B] ZP_05544813.1 0.034 23% ...

  15. Amphibian (Xenopus sp.) iodothyronine deiodinase ...

    Science.gov (United States)

    The U.S. EPA-MED amphibian thyroid group is currently screening chemicals for inhibition of human iodothyronine deiodinase activity as components of the thyroid system important in human development. Amphibians are a bellwether taxonomic group to gauge toxicity of chemicals in the environment. Amphibian thyroid function is not only important in development but also metamorphosis. Xenopus sp. have been used extensively as model organisms and are well characterized genetically. We propose to screen a list of chemicals (selected from the human DIO screening results) to test for inhibition of Xenopus deiodinases. Large quantities of the enzymes will be produced using an adenovirus system. Our preliminary results show that there may be catalytic differences between human and Xenopus deiodinases. The Twin Ports Early Career Scientists is a new group formed within the Duluth-Superior scientific community. This presentation will provide a basic introduction to my research and our mission at EPA, and help to establish networking and collaboration relationships across disciplines and institutions.

  16. Detection of Salmonella sp., Vibrio sp. and total plate count bacteria on blood cockle (Anadara granosa)

    Science.gov (United States)

    Ekawati, ER; Yusmiati, S. N. H.

    2018-01-01

    Blood cockle (Anadara granosa) has high level of zinc and protein, which is beneficial for therapeutic function for malnourished particularly stunting case in children. Zinc in animal foods is more absorbable than that from vegetable food. Blood cockle (Anadara granosa) is rich in nutrient and an excellent environment for the growth of microorganisms. This research aimed to identify the contamination of Salmonella sp., Vibrio sp. and total plate count bacteria on blood cockle (Anadara granosa). This was observation research with laboratory analysis. Salmonella sp. and Vibrio sp. were detected from blood cockle. Total plate count was determine of the total amount of the bacteria. Results detected from 20 samples of blood cockle showed that all samples were negative of Salmonella sp. and 1 sample positive Vibrio sp. The result of total plate count bacteria was sample.

  17. A mollusk VDR/PXR/CAR-like (NR1J) nuclear receptor provides insight into ancient detoxification mechanisms

    International Nuclear Information System (INIS)

    Cruzeiro, Catarina; Lopes-Marques, Mónica; Ruivo, Raquel; Rodrigues-Oliveira, Nádia; Santos, Miguel M.; Rocha, Maria João; Rocha, Eduardo; Castro, L. Filipe C.

    2016-01-01

    Highlights: • A nuclear receptor orthologue of the NR1J group is isolated from a mollusc. • The molluscan NR1J transactivates gene expression upon exposure to okadaic acid but not a pesticide, esfenvarelate and triclosan. • Lineage specific gene duplications and gene loss have occurred in the NR1J of protostomes with likely impacts on detoxification mechanisms. - Abstract: The origin and diversification of the metazoan endocrine systems represents a fundamental research issue in biology. Nuclear receptors are critical components of these systems. A particular group named VDR/PXR/CAR (NR1I/J) is central in the mediation of detoxification responses. While orthologues have been thoroughly characterized in vertebrates, a sparse representation is currently available for invertebrates. Here, we provide the first isolation and characterization of a lophotrochozoan protostome VDR/PXR/CAR nuclear receptor (NR1J), in the estuarine bivalve the peppery furrow shell (Scrobicularia plana). Using a reporter gene assay, we evaluated the xenobiotic receptor plasticity comparing the human PXR with the S. plana NR1Jβ. Our results show that the molluscan receptor responds to a natural toxin (okadaic acid) in a similar fashion to that reported for other invertebrates. In contrast, the pesticide esfenvalerate displayed a unique response, since it down regulated transactivation at higher concentrations, while for triclosan no response was observed. Additionally, we uncovered lineage specific gene duplications and gene loss in the gene group encoding NRs in protostomes with likely impacts on the complexity of detoxification mechanisms across different phyla. Our findings pave the way for the development of multi-specific sensor tools to screen xenobiotic compounds acting via the NR1I/J group.

  18. Involvement of nitrate reductase (NR) in osmotic stress-induced NO generation of Arabidopsis thaliana L. roots.

    Science.gov (United States)

    Kolbert, Zsuzsanna; Ortega, Leandro; Erdei, László

    2010-01-01

    Nitric oxide (NO) is undoubtedly a potential signal molecule in diverse developmental processes and stress responses. Despite our extensive knowledge about the role of NO in physiological and stress responses, the source of this gaseous molecule is still unresolved. The aim of this study was to investigate the potential role of nitrate reductase (NR) as the source of NO accumulation in the root system of wild-type and NR-deficient nia1, nia2 mutant Arabidopsis plants under osmotic stress conditions induced by a polyethylene glycol (PEG 6000) treatment. Reduction of primary root (PR) length was detected as the effect of osmotic stress in wild-type and NR-deficient plants. We found that osmotic stress-induced lateral root (LR) initiation in wild-type, but not in NR-mutant plants. High levels of NO formation occurred in roots of Col-1 plants as the effect of PEG treatment. The mammalian nitric oxide synthase (NOS) inhibitor N(G)-monomethyl-L-arginine (L-NMMA) had no effect on LR initiation or NO generation, while tungstate, an NR inhibitor, inhibited the later phase of osmotic stress-induced NO accumulation and slightly decreased the LR development. In nia1, nia2 roots, the PEG treatment induced the first phase of NO production, but later NO production was inhibited. We conclude that the first phase of PEG-induced NO generation is not dependent on NOS-like or NR activity. It is also suggested that the activity of NR in roots is required for the later phase of osmotic stress-induced NO formation.

  19. Enhanced Expression of NR2B Subunits of NMDA Receptors in the Inherited Glaucomatous DBA/2J Mouse Retina

    Directory of Open Access Journals (Sweden)

    Ling-Dan Dong

    2013-01-01

    Full Text Available DBA/2J mouse has been used as a model for spontaneous secondary glaucoma. Here, we investigated changes in expression of NMDA receptor (NMDAR subunits and Cdk5/p35/NMDAR signaling in retinas of DBA/2J mice using Western blot technique. The protein levels of NR1 and NR2A subunits in retinas of DBA/2J mice at all ages (6–12 months were not different from those in age-matched C57BL/6 mice. In contrast, the protein levels of NR2B subunits, in addition to age-dependent change, significantly increased with elevated intraocular pressure (IOP in DBA/2J mice at 6 and 9 months as compared with age-matched controls. Moreover, expression of Cdk5, p35 and ratio of p-NR2AS1232/NR2A progressively increased with time in both strains, suggestive of activated Cdk5/p35 signaling pathway. However, the changes in these proteins were in the same levels in both strain mice, except a significant increase of p35 proteins at 6 months in DBA/2J mice. Meanwhile, the protein levels of Brn-3a, a retinal ganglion cell (RGC maker, remarkably decreased at 9–12 months in DBA/2J mice, which was in parallel with the changes of NR2B expression. Our results suggest that elevated IOP-induced increase in expression of NR2B subunits of NMDARs may be involved in RGC degeneration of DBA/2J mice.

  20. Kalirin Binds the NR2B Subunit of the NMDA Receptor, Altering Its Synaptic Localization and Function

    KAUST Repository

    Kiraly, D. D.

    2011-08-31

    The ability of dendritic spines to change size and shape rapidly is critical in modulating synaptic strength; these morphological changes are dependent upon rearrangements of the actin cytoskeleton. Kalirin-7 (Kal7), a Rho guanine nucleotide exchange factor localized to the postsynaptic density (PSD), modulates dendritic spine morphology in vitro and in vivo. Kal7 activates Rac and interacts with several PSD proteins, including PSD-95, DISC-1, AF-6, and Arf6. Mice genetically lacking Kal7 (Kal7KO) exhibit deficient hippocampal long-term potentiation (LTP) as well as behavioral abnormalities in models of addiction and learning. Purified PSDs from Kal7KO mice contain diminished levels of NR2B, an NMDA receptor subunit that plays a critical role in LTP induction. Here we demonstrate that Kal7KO animals have decreased levels of NR2B-dependent NMDA receptor currents in cortical pyramidal neurons as well as a specific deficit in cell surface expression of NR2B. Additionally, we demonstrate that the genotypic differences in conditioned place preference and passive avoidance learning seen in Kal7KO mice are abrogated when animals are treated with an NR2B-specific antagonist during conditioning. Finally, we identify a stable interaction between the pleckstrin homology domain of Kal7 and the juxtamembrane region of NR2B preceding its cytosolic C-terminal domain. Binding of NR2B to a protein that modulates the actin cytoskeleton is important, as NMDA receptors require actin integrity for synaptic localization and function. These studies demonstrate a novel and functionally important interaction between the NR2B subunit of the NMDA receptor and Kalirin, proteins known to be essential for normal synaptic plasticity.

  1. A mollusk VDR/PXR/CAR-like (NR1J) nuclear receptor provides insight into ancient detoxification mechanisms

    Energy Technology Data Exchange (ETDEWEB)

    Cruzeiro, Catarina, E-mail: catarinarcruzeiro@hotmail.com [ICBAS - Institute of Biomedical Sciences Abel Salazar, U. Porto - University of Porto (Portugal); CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); Lopes-Marques, Mónica, E-mail: monicaslm@hotmail.com [ICBAS - Institute of Biomedical Sciences Abel Salazar, U. Porto - University of Porto (Portugal); CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); Ruivo, Raquel, E-mail: ruivo.raquel@gmail.com [CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); Rodrigues-Oliveira, Nádia, E-mail: nadia.oliveira@ciimar.up.pt [CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); Santos, Miguel M., E-mail: santos@ciimar.up.pt [CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); FCUP - Faculty of Sciences, Department of Biology, U. Porto (Portugal); Rocha, Maria João, E-mail: mjsrocha@netcabo.pt [ICBAS - Institute of Biomedical Sciences Abel Salazar, U. Porto - University of Porto (Portugal); CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); Rocha, Eduardo, E-mail: erocha@icbas.up.pt [ICBAS - Institute of Biomedical Sciences Abel Salazar, U. Porto - University of Porto (Portugal); CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); Castro, L. Filipe C., E-mail: filipe.castro@ciimar.up.pt [CIIMAR/CIMAR - Interdisciplinary Center of Marine and Environmental Research, U. Porto (Portugal); FCUP - Faculty of Sciences, Department of Biology, U. Porto (Portugal)

    2016-05-15

    Highlights: • A nuclear receptor orthologue of the NR1J group is isolated from a mollusc. • The molluscan NR1J transactivates gene expression upon exposure to okadaic acid but not a pesticide, esfenvarelate and triclosan. • Lineage specific gene duplications and gene loss have occurred in the NR1J of protostomes with likely impacts on detoxification mechanisms. - Abstract: The origin and diversification of the metazoan endocrine systems represents a fundamental research issue in biology. Nuclear receptors are critical components of these systems. A particular group named VDR/PXR/CAR (NR1I/J) is central in the mediation of detoxification responses. While orthologues have been thoroughly characterized in vertebrates, a sparse representation is currently available for invertebrates. Here, we provide the first isolation and characterization of a lophotrochozoan protostome VDR/PXR/CAR nuclear receptor (NR1J), in the estuarine bivalve the peppery furrow shell (Scrobicularia plana). Using a reporter gene assay, we evaluated the xenobiotic receptor plasticity comparing the human PXR with the S. plana NR1Jβ. Our results show that the molluscan receptor responds to a natural toxin (okadaic acid) in a similar fashion to that reported for other invertebrates. In contrast, the pesticide esfenvalerate displayed a unique response, since it down regulated transactivation at higher concentrations, while for triclosan no response was observed. Additionally, we uncovered lineage specific gene duplications and gene loss in the gene group encoding NRs in protostomes with likely impacts on the complexity of detoxification mechanisms across different phyla. Our findings pave the way for the development of multi-specific sensor tools to screen xenobiotic compounds acting via the NR1I/J group.

  2. Helicotylenchus stylocercus n. sp. and Rotylenchus phaliurus n. sp. (Nematoda: Hoplolaimidae) from Costa Rica.

    Science.gov (United States)

    Siddiqi, M R; Pinochet, J

    1979-10-01

    Two new species of plant-parasitic nematodes from Costa Rica are described. Helicotygenchus styloeercus n. sp., from soil around roots of banana at Coto, is distinguished hy the female tail, which bears a large pillarlike ventral projection. Rotylenchus phaliurus n. sp., from soil artmnd roots of Dioscoroea sp. at Sixaola, differs from R. caudaphasmidius in having the conus equal to or more than half the spear length, and large terminal annules on the female tail.

  3. EOP Gold Coral (Gerardia sp.) Growth Measurements

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Gold coral (Gerardia sp.) trees that were inspected years earlier on Pisces submersible dives were revisited and their change in size measured. The fishery for...

  4. Silencing the SpMPK1, SpMPK2, and SpMPK3 Genes in Tomato Reduces Abscisic Acid—Mediated Drought Tolerance

    Directory of Open Access Journals (Sweden)

    Yan Liang

    2013-11-01

    Full Text Available Drought is a major threat to agriculture production worldwide. Mitogen-activated protein kinases (MAPKs play a pivotal role in sensing and converting stress signals into appropriate responses so that plants can adapt and survive. To examine the function of MAPKs in the drought tolerance of tomato plants, we silenced the SpMPK1, SpMPK2, and SpMPK3 genes in wild-type plants using the virus-induced gene silencing (VIGS method. The results indicate that silencing the individual genes or co-silencing SpMPK1, SpMPK2, and SpMPK3 reduced the drought tolerance of tomato plants by varying degrees. Co-silencing SpMPK1 and SpMPK2 impaired abscisic acid (ABA-induced and hydrogen peroxide (H2O2-induced stomatal closure and enhanced ABA-induced H2O2 production. Similar results were observed when silencing SpMPK3 alone, but not when SpMPK1 and SpMPK2 were individually silenced. These data suggest that the functions of SpMPK1 and SpMPK2 are redundant, and they overlap with that of SpMPK3 in drought stress signaling pathways. In addition, we found that SpMPK3 may regulate H2O2 levels by mediating the expression of CAT1. Hence, SpMPK1, SpMPK2, and SpMPK3 may play crucial roles in enhancing tomato plants’ drought tolerance by influencing stomatal activity and H2O2 production via the ABA-H2O2 pathway.

  5. NCBI nr-aa BLAST: CBRC-FCAT-01-1010 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-1010 ref|ZP_01998680.1| DNA internalization-related competence protein... ComEC/Rec2 [Beggiatoa sp. PS] gb|EDN71312.1| DNA internalization-related competence protein ComEC/Rec2 [Beggiatoa sp. PS] ZP_01998680.1 3.3 21% ...

  6. NCBI nr-aa BLAST: CBRC-MLUC-01-0287 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MLUC-01-0287 ref|YP_002950411.1| DNA internalization-related competence protei...n ComEC/Rec2 [Geobacillus sp. WCH70] gb|ACS25145.1| DNA internalization-related competence protein ComEC/Rec2 [Geobacillus sp. WCH70] YP_002950411.1 0.019 28% ...

  7. NCBI nr-aa BLAST: CBRC-BTAU-01-2379 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-BTAU-01-2379 ref|YP_001354020.1| DNA polymerase III gamma and tau subunit [Janthinobacterium sp. Mars...eille] gb|ABR88343.1| DNA polymerase III gamma and tau subunit [Janthinobacterium sp. Marseille] YP_001354020.1 0.022 34% ...

  8. NCBI nr-aa BLAST: CBRC-CREM-01-1367 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CREM-01-1367 ref|YP_001352545.1| hypothetical protein mma_0855 [Janthinobacterium sp. Mars...eille] gb|ABR90600.1| Uncharacterized conserved protein [Janthinobacterium sp. Marseille] YP_001352545.1 2e-58 46% ...

  9. NCBI nr-aa BLAST: CBRC-XTRO-01-0435 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0435 ref|YP_001354525.1| hypothetical protein mma_2835 [Janthinobacterium sp. Mars...eille] gb|ABR91058.1| Uncharacterized conserved protein [Janthinobacterium sp. Marseille] YP_001354525.1 3e-38 28% ...

  10. NCBI nr-aa BLAST: CBRC-DNOV-01-3183 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-3183 ref|YP_001353955.1| hypothetical protein mma_2265 [Janthinobacterium sp. Mars...eille] gb|ABR91661.1| Uncharacterized conserved protein [Janthinobacterium sp. Marseille] YP_001353955.1 8.9 29% ...

  11. NCBI nr-aa BLAST: CBRC-XTRO-01-0593 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0593 ref|YP_001354210.1| MviN, virulence factor (MOP family) [Janthinobacterium sp. Mars...eille] gb|ABR88276.1| MviN, virulence factor (MOP family) [Janthinobacterium sp. Marseille] YP_001354210.1 2e-95 58% ...

  12. NCBI nr-aa BLAST: CBRC-XTRO-01-0450 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0450 ref|YP_001352799.1| sulfate permease, SulP family [Janthinobacterium sp. Mars...eille] gb|ABR89163.1| sulfate permease, SulP family [Janthinobacterium sp. Marseille] YP_001352799.1 1e-101 48% ...

  13. NCBI nr-aa BLAST: CBRC-CREM-01-1340 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CREM-01-1340 ref|YP_001354545.1| hypothetical protein mma_2855 [Janthinobacterium sp. Mars...eille] gb|ABR91786.1| Uncharacterized conserved protein [Janthinobacterium sp. Marseille] YP_001354545.1 7e-12 26% ...

  14. NCBI nr-aa BLAST: CBRC-ETEL-01-0451 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ETEL-01-0451 ref|YP_001354943.1| transporter of the MFS superfamily [Janthinobacterium sp. Mars...eille] gb|ABR90863.1| transporter of the MFS superfamily [Janthinobacterium sp. Marseille] YP_001354943.1 3.2 39% ...

  15. NCBI nr-aa BLAST: CBRC-PMAR-01-0172 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PMAR-01-0172 ref|YP_001351942.1| hypothetical protein mma_0252 [Janthinobacterium sp. Mars...eille] gb|ABR89731.1| Uncharacterized conserved protein [Janthinobacterium sp. Marseille] YP_001351942.1 2.2 33% ...

  16. NCBI nr-aa BLAST: CBRC-XTRO-01-1396 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-1396 ref|YP_001355012.1| hypothetical protein mma_3322 [Janthinobacterium sp. Mars...eille] gb|ABR91206.1| Uncharacterized conserved protein [Janthinobacterium sp. Marseille] YP_001355012.1 2e-11 32% ...

  17. NCBI nr-aa BLAST: CBRC-XTRO-01-0704 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0704 ref|YP_001354672.1| two-component system sensor kinase [Janthinobacterium sp. Mars...eille] gb|ABR89078.1| two-component system sensor kinase [Janthinobacterium sp. Marseille] YP_001354672.1 6e-30 28% ...

  18. NCBI nr-aa BLAST: CBRC-BTAU-01-1365 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-BTAU-01-1365 ref|YP_001352782.1| hypothetical protein mma_1092 [Janthinobacterium sp. Mars...eille] gb|ABR89673.1| Uncharacterized conserved protein [Janthinobacterium sp. Marseille] YP_001352782.1 9.9 30% ...

  19. NCBI nr-aa BLAST: CBRC-ACAR-01-0160 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-0160 ref|YP_001353164.1| NADH dehydrogenase I chain M [Janthinobacterium sp. Mars...eille] gb|ABR88792.1| NADH dehydrogenase I chain M [Janthinobacterium sp. Marseille] YP_001353164.1 0.081 22% ...

  20. NCBI nr-aa BLAST: CBRC-TBEL-01-0950 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TBEL-01-0950 ref|YP_479163.1| hypothetical protein Francci3_0040 [Frankia sp. ...CcI3] gb|ABD09434.1| hypothetical protein Francci3_0040 [Frankia sp. CcI3] YP_479163.1 5.8 37% ...

  1. NCBI nr-aa BLAST: CBRC-PHAM-01-0706 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PHAM-01-0706 ref|YP_001504998.1| hypothetical protein Franean1_0633 [Frankia s...p. EAN1pec] gb|ABW10092.1| conserved hypothetical protein [Frankia sp. EAN1pec] YP_001504998.1 0.63 35% ...

  2. NCBI nr-aa BLAST: CBRC-DSIM-06-0019 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DSIM-06-0019 ref|YP_482035.1| NAD(P) transhydrogenase, beta subunit [Frankia s...p. CcI3] gb|ABD12306.1| NAD(P) transhydrogenase, beta subunit [Frankia sp. CcI3] YP_482035.1 0.064 28% ...

  3. NCBI nr-aa BLAST: CBRC-PABE-21-0021 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PABE-21-0021 ref|YP_001509563.1| polysaccharide biosynthesis protein [Frankia ...sp. EAN1pec] gb|ABW14657.1| polysaccharide biosynthesis protein [Frankia sp. EAN1pec] YP_001509563.1 0.67 27% ...

  4. NCBI nr-aa BLAST: CBRC-CREM-01-1275 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CREM-01-1275 ref|YP_001510781.1| heavy metal translocating P-type ATPase [Franki...a sp. EAN1pec] gb|ABW15875.1| heavy metal translocating P-type ATPase [Frankia sp. EAN1pec] YP_001510781.1 1e-118 72% ...

  5. NCBI nr-aa BLAST: CBRC-DNOV-01-2234 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-2234 ref|YP_482643.1| translation initiation factor IF-2 [Frankia sp. ...CcI3] gb|ABD12914.1| bacterial translation initiation factor 2 (bIF-2) [Frankia sp. CcI3] YP_482643.1 7e-05 31% ...

  6. NCBI nr-aa BLAST: CBRC-TNIG-09-0033 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-09-0033 ref|YP_001510423.1| hypothetical protein Franean1_6173 [Frankia s...p. EAN1pec] gb|ABW15517.1| hypothetical protein Franean1_6173 [Frankia sp. EAN1pec] YP_001510423.1 0.097 29% ...

  7. NCBI nr-aa BLAST: CBRC-HSAP-08-0029 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-HSAP-08-0029 ref|YP_001507265.1| hypothetical protein Franean1_2939 [Frankia s...p. EAN1pec] gb|ABW12359.1| hypothetical protein Franean1_2939 [Frankia sp. EAN1pec] YP_001507265.1 2.8 30% ...

  8. NCBI nr-aa BLAST: CBRC-LAFR-01-0782 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-0782 ref|YP_001507559.1| hypothetical protein Franean1_3247 [Frankia s...p. EAN1pec] gb|ABW12653.1| hypothetical protein Franean1_3247 [Frankia sp. EAN1pec] YP_001507559.1 2.6 26% ...

  9. NCBI nr-aa BLAST: CBRC-SARA-01-0899 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-SARA-01-0899 ref|YP_001504686.1| conserved hypothetical protein [Frankia sp. E...AN1pec] gb|ABW09780.1| conserved hypothetical protein [Frankia sp. EAN1pec] YP_001504686.1 0.006 32% ...

  10. NCBI nr-aa BLAST: CBRC-RNOR-23-0023 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-23-0023 ref|YP_001504861.1| WD40 domain protein beta Propeller [Frankia s...p. EAN1pec] gb|ABW09955.1| WD40 domain protein beta Propeller [Frankia sp. EAN1pec] YP_001504861.1 5.2 42% ...

  11. NCBI nr-aa BLAST: CBRC-CFAM-08-0030 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CFAM-08-0030 ref|YP_001510350.1| serine/threonine protein kinase [Frankia sp. ...EAN1pec] gb|ABW15444.1| serine/threonine protein kinase [Frankia sp. EAN1pec] YP_001510350.1 2e-04 31% ...

  12. NCBI nr-aa BLAST: CBRC-RMAC-14-0006 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RMAC-14-0006 ref|YP_001510095.1| putative integral membrane protein [Frankia s...p. EAN1pec] gb|ABW15189.1| putative integral membrane protein [Frankia sp. EAN1pec] YP_001510095.1 7e-04 32% ...

  13. NCBI nr-aa BLAST: CBRC-RMAC-14-0006 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RMAC-14-0006 ref|YP_001507126.1| hypothetical protein Franean1_2797 [Frankia s...p. EAN1pec] gb|ABW12220.1| hypothetical protein Franean1_2797 [Frankia sp. EAN1pec] YP_001507126.1 0.002 33% ...

  14. NCBI nr-aa BLAST: CBRC-PABE-03-0043 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PABE-03-0043 ref|YP_001510079.1| putative PAS/PAC sensor protein [Frankia sp. ...EAN1pec] gb|ABW15173.1| putative PAS/PAC sensor protein [Frankia sp. EAN1pec] YP_001510079.1 1.6 34% ...

  15. NCBI nr-aa BLAST: CBRC-GGOR-01-1392 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GGOR-01-1392 ref|YP_481595.1| electron transport protein SCO1/SenC [Frankia sp.... CcI3] gb|ABD11866.1| electron transport protein SCO1/SenC [Frankia sp. CcI3] YP_481595.1 7.7 35% ...

  16. NCBI nr-aa BLAST: CBRC-PABE-01-0118 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PABE-01-0118 ref|YP_001505093.1| conserved hypothetical protein [Frankia sp. E...AN1pec] gb|ABW10187.1| conserved hypothetical protein [Frankia sp. EAN1pec] YP_001505093.1 0.14 27% ...

  17. NCBI nr-aa BLAST: CBRC-TGUT-08-0033 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TGUT-08-0033 ref|YP_001506555.1| hypothetical protein Franean1_2214 [Frankia s...p. EAN1pec] gb|ABW11649.1| hypothetical protein Franean1_2214 [Frankia sp. EAN1pec] YP_001506555.1 0.011 32% ...

  18. NCBI nr-aa BLAST: CBRC-OSAT-11-0006 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OSAT-11-0006 ref|YP_001509478.1| NADH dehydrogenase (quinone) [Frankia sp. EAN...1pec] gb|ABW14572.1| NADH dehydrogenase (quinone) [Frankia sp. EAN1pec] YP_001509478.1 0.001 25% ...

  19. NCBI nr-aa BLAST: CBRC-OSAT-01-0045 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OSAT-01-0045 ref|YP_001508080.1| 2-hydroxy-3-oxopropionate reductase [Frankia ...sp. EAN1pec] gb|ABW13174.1| 2-hydroxy-3-oxopropionate reductase [Frankia sp. EAN1pec] YP_001508080.1 1e-78 54% ...

  20. NCBI nr-aa BLAST: CBRC-FCAT-01-1004 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-1004 ref|YP_001505093.1| conserved hypothetical protein [Frankia sp. E...AN1pec] gb|ABW10187.1| conserved hypothetical protein [Frankia sp. EAN1pec] YP_001505093.1 0.004 26% ...

  1. NCBI nr-aa BLAST: CBRC-LAFR-01-1270 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-1270 ref|YP_480537.1| hypothetical protein Francci3_1431 [Frankia sp. ...CcI3] gb|ABD10808.1| hypothetical protein Francci3_1431 [Frankia sp. CcI3] YP_480537.1 3.8 26% ...

  2. NCBI nr-aa BLAST: CBRC-RNOR-23-0082 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-23-0082 ref|YP_001508043.1| serine/threonine protein kinase with WD40 repeats [Franki...a sp. EAN1pec] gb|ABW13137.1| serine/threonine protein kinase with WD40 repeats [Frankia sp. EAN1pec] YP_001508043.1 6.3 36% ...

  3. NCBI nr-aa BLAST: CBRC-CJAC-01-0336 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-0336 ref|YP_001505542.1| translation initiation factor IF-2 [Frankia s...p. EAN1pec] gb|ABW10636.1| translation initiation factor IF-2 [Frankia sp. EAN1pec] YP_001505542.1 4e-07 35% ...

  4. NCBI nr-aa BLAST: CBRC-MMUR-01-1348 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUR-01-1348 ref|YP_001505846.1| diacylglycerol kinase catalytic region [Franki...a sp. EAN1pec] gb|ABW10940.1| diacylglycerol kinase catalytic region [Frankia sp. EAN1pec] YP_001505846.1 0.008 30% ...

  5. NCBI nr-aa BLAST: CBRC-CJAC-01-0336 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-0336 ref|YP_482643.1| translation initiation factor IF-2 [Frankia sp. ...CcI3] gb|ABD12914.1| bacterial translation initiation factor 2 (bIF-2) [Frankia sp. CcI3] YP_482643.1 8e-07 32% ...

  6. NCBI nr-aa BLAST: CBRC-ACAR-01-1054 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-1054 ref|ZP_01169668.1| conserved membrane-spanning protein [Bacillus ...sp. NRRL B-14911] gb|EAR67900.1| conserved membrane-spanning protein [Bacillus sp. NRRL B-14911] ZP_01169668.1 1.3 33% ...

  7. NCBI nr-aa BLAST: CBRC-ACAR-01-0955 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-0955 ref|ZP_01169668.1| conserved membrane-spanning protein [Bacillus ...sp. NRRL B-14911] gb|EAR67900.1| conserved membrane-spanning protein [Bacillus sp. NRRL B-14911] ZP_01169668.1 2.3 33% ...

  8. NCBI nr-aa BLAST: CBRC-CREM-01-1327 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CREM-01-1327 ref|YP_705999.1| possible ABC antibiotics transporter [Rhodococcu...s sp. RHA1] gb|ABG97841.1| possible ABC antibiotics transporter [Rhodococcus sp. RHA1] YP_705999.1 3e-47 39% ...

  9. NCBI nr-aa BLAST: CBRC-DSIM-04-0090 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DSIM-04-0090 ref|YP_922866.1| putative ABC antibiotics transporter [Nocardioid...es sp. JS614] gb|ABL81179.1| putative ABC antibiotics transporter [Nocardioides sp. JS614] YP_922866.1 0.003 31% ...

  10. NCBI nr-aa BLAST: CBRC-CREM-01-1327 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CREM-01-1327 ref|YP_922866.1| putative ABC antibiotics transporter [Nocardioid...es sp. JS614] gb|ABL81179.1| putative ABC antibiotics transporter [Nocardioides sp. JS614] YP_922866.1 3e-41 36% ...

  11. NCBI nr-aa BLAST: CBRC-OPRI-01-0271 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OPRI-01-0271 ref|ZP_01826755.1| pneumococcal surface protein A [Streptococcus pneu...moniae SP14-BS69] gb|EDK67229.1| pneumococcal surface protein A [Streptococcus pneumoniae SP14-BS69] ZP_01826755.1 3e-05 29% ...

  12. NCBI nr-aa BLAST: CBRC-OPRI-01-0271 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OPRI-01-0271 ref|ZP_02713591.1| pneumococcal surface protein A [Streptococcus pneu...moniae SP195] gb|EDT92575.1| pneumococcal surface protein A [Streptococcus pneumoniae SP195] ZP_02713591.1 4e-05 29% ...

  13. NCBI nr-aa BLAST: CBRC-OPRI-01-0271 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OPRI-01-0271 ref|ZP_01822350.1| pneumococcal surface protein A [Streptococcus pneu...moniae SP9-BS68] gb|EDK79448.1| pneumococcal surface protein A [Streptococcus pneumoniae SP9-BS68] ZP_01822350.1 4e-05 29% ...

  14. NCBI nr-aa BLAST: CBRC-TTRU-01-0816 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0816 ref|YP_003256804.1| cell cycle protein [Blattabacterium sp. (Periplanet...a americana) str. BPLAN] gb|ACX83661.1| cell cycle protein [Blattabacterium sp. (Periplaneta americana) str. BPLAN] YP_003256804.1 0.002 27% ...

  15. NCBI nr-aa BLAST: CBRC-LAFR-01-1781 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-1781 ref|YP_833238.1| hypothetical protein Arth_3763 [Arthrobacter sp.... FB24] gb|ABK05138.1| hypothetical protein Arth_3763 [Arthrobacter sp. FB24] YP_833238.1 3.3 25% ...

  16. NCBI nr-aa BLAST: CBRC-RMAC-14-0300 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RMAC-14-0300 ref|YP_832933.1| hypothetical protein Arth_3458 [Arthrobacter sp.... FB24] gb|ABK04833.1| hypothetical protein Arth_3458 [Arthrobacter sp. FB24] YP_832933.1 0.38 24% ...

  17. NCBI nr-aa BLAST: CBRC-LAFR-01-0212 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-0212 ref|YP_833238.1| hypothetical protein Arth_3763 [Arthrobacter sp.... FB24] gb|ABK05138.1| hypothetical protein Arth_3763 [Arthrobacter sp. FB24] YP_833238.1 0.48 31% ...

  18. NCBI nr-aa BLAST: CBRC-FCAT-01-1212 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-1212 ref|YP_001379363.1| Pyrrolo-quinoline quinone [Anaeromyxobacter s...p. Fw109-5] gb|ABS26379.1| Pyrrolo-quinoline quinone [Anaeromyxobacter sp. Fw109-5] YP_001379363.1 0.041 27% ...

  19. NCBI nr-aa BLAST: CBRC-ACAR-01-1198 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-1198 ref|YP_001280665.1| Pyrrolo-quinoline quinone [Psychrobacter sp. ...PRwf-1] gb|ABQ94715.1| Pyrrolo-quinoline quinone [Psychrobacter sp. PRwf-1] YP_001280665.1 6.8 30% ...

  20. NCBI nr-aa BLAST: CBRC-XTRO-01-3176 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-3176 ref|ZP_01618818.1| Abortive infection protein [Lyngbya sp. PCC 81...06] gb|EAW39293.1| Abortive infection protein [Lyngbya sp. PCC 8106] ZP_01618818.1 0.087 25% ...

  1. NCBI nr-aa BLAST: CBRC-TNIG-22-0321 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-22-0321 ref|YP_001175563.1| amino acid permease-associated region [Enterobacter... sp. 638] gb|ABP59512.1| amino acid permease-associated region [Enterobacter sp. 638] YP_001175563.1 0.21 27% ...

  2. NCBI nr-aa BLAST: CBRC-RNOR-06-0041 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-06-0041 ref|YP_001177081.1| sodium/hydrogen exchanger [Enterobacter sp. 6...38] gb|ABP61030.1| sodium/hydrogen exchanger [Enterobacter sp. 638] YP_001177081.1 6.8 30% ...

  3. NCBI nr-aa BLAST: CBRC-DDIS-03-0079 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DDIS-03-0079 ref|YP_001165540.1| major facilitator superfamily MFS_1 [Enterobacter... sp. 638] gb|ABP62866.1| major facilitator superfamily MFS_1 [Enterobacter sp. 638] YP_001165540.1 7e-05 25% ...

  4. NCBI nr-aa BLAST: CBRC-CREM-01-1348 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CREM-01-1348 ref|YP_001176637.1| protein of unknown function UPF0118 [Enterobacter... sp. 638] gb|ABP60586.1| protein of unknown function UPF0118 [Enterobacter sp. 638] YP_001176637.1 7e-41 30% ...

  5. NCBI nr-aa BLAST: CBRC-RNOR-02-0044 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-02-0044 ref|YP_001165531.1| hypothetical protein Ent638_4252 [Enterobacter... sp. 638] gb|ABP62857.1| hypothetical protein Ent638_4252 [Enterobacter sp. 638] YP_001165531.1 0.002 26% ...

  6. NCBI nr-aa BLAST: CBRC-XTRO-01-0063 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0063 ref|YP_001176625.1| major facilitator superfamily MFS_1 [Enterobacter... sp. 638] gb|ABP60574.1| major facilitator superfamily MFS_1 [Enterobacter sp. 638] YP_001176625.1 9e-66 50% ...

  7. NCBI nr-aa BLAST: CBRC-CBRE-01-0703 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CBRE-01-0703 ref|YP_001174751.1| d-galactonate transporter [Enterobacter sp. 6...38] gb|ABP58700.1| d-galactonate transporter [Enterobacter sp. 638] YP_001174751.1 3.6 22% ...

  8. NCBI nr-aa BLAST: CBRC-CREM-01-1314 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CREM-01-1314 ref|YP_001175819.1| major facilitator superfamily MFS_1 [Enterobacter... sp. 638] gb|ABP59768.1| major facilitator superfamily MFS_1 [Enterobacter sp. 638] YP_001175819.1 7e-37 32% ...

  9. NCBI nr-aa BLAST: CBRC-FRUB-02-0691 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FRUB-02-0691 ref|YP_001165531.1| hypothetical protein Ent638_4252 [Enterobacter... sp. 638] gb|ABP62857.1| hypothetical protein Ent638_4252 [Enterobacter sp. 638] YP_001165531.1 7e-12 38% ...

  10. NCBI nr-aa BLAST: CBRC-MMUS-06-0145 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0145 ref|YP_001177656.1| protein of unknown function DUF540 [Enterobacter... sp. 638] gb|ABP61605.1| protein of unknown function DUF540 [Enterobacter sp. 638] YP_001177656.1 9.0 24% ...

  11. NCBI nr-aa BLAST: CBRC-TGUT-37-0248 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TGUT-37-0248 ref|YP_001165531.1| hypothetical protein Ent638_4252 [Enterobacter... sp. 638] gb|ABP62857.1| hypothetical protein Ent638_4252 [Enterobacter sp. 638] YP_001165531.1 4.6 27% ...

  12. NCBI nr-aa BLAST: CBRC-ACAR-01-0270 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-0270 ref|ZP_01849772.1| tungsten-containing formylmethanofuran dehydro...genase, subunit B [Methylobacterium sp. 4-46] gb|EDK91743.1| tungsten-containing formylmethanofuran dehydrogenase, subunit B [Methylobacterium sp. 4-46] ZP_01849772.1 3e-04 32% ...

  13. NCBI nr-aa BLAST: CBRC-CREM-01-1363 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CREM-01-1363 ref|YP_708076.1| probable metabolite transporter, MFS superfamily [Rhodococcus... sp. RHA1] gb|ABG99918.1| probable metabolite transporter, MFS superfamily protein [Rhodococcus sp. RHA1] YP_708076.1 2e-62 40% ...

  14. NCBI nr-aa BLAST: CBRC-SARA-01-1099 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-SARA-01-1099 ref|YP_708759.1| hypothetical protein RHA1_ro10412 [Rhodococcus s...p. RHA1] gb|ABH00601.1| conserved hypothetical protein [Rhodococcus sp. RHA1] YP_708759.1 2.7 25% ...

  15. NCBI nr-aa BLAST: CBRC-TGUT-35-0000 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TGUT-35-0000 ref|YP_706575.1| translation initiation factor IF-2 [Rhodococcus ...sp. RHA1] gb|ABG98417.1| translation initiation factor IF-2 [Rhodococcus sp. RHA1] YP_706575.1 8e-07 28% ...

  16. NCBI nr-aa BLAST: CBRC-EEUR-01-0548 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-EEUR-01-0548 ref|YP_708349.1| hypothetical protein RHA1_ro09148 [Rhodococcus s...p. RHA1] gb|ABH00191.1| conserved hypothetical protein [Rhodococcus sp. RHA1] YP_708349.1 0.028 33% ...

  17. NCBI nr-aa BLAST: CBRC-CPOR-01-0137 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CPOR-01-0137 ref|YP_700499.1| serine/threonine-protein kinase [Rhodococcus sp.... RHA1] gb|ABG92341.1| serine/threonine-protein kinase [Rhodococcus sp. RHA1] YP_700499.1 0.19 26% ...

  18. NCBI nr-aa BLAST: CBRC-RMAC-01-0032 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RMAC-01-0032 ref|YP_703453.1| possible xanthine/uracil permease [Rhodococcus s...p. RHA1] gb|ABG95295.1| possible xanthine/uracil permease [Rhodococcus sp. RHA1] YP_703453.1 0.37 21% ...

  19. NCBI nr-aa BLAST: CBRC-LAFR-01-0521 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-0521 ref|YP_704846.1| transporter, MFS superfamily [Rhodococcus sp. RH...A1] gb|ABG96688.1| transporter, MFS superfamily protein [Rhodococcus sp. RHA1] YP_704846.1 1.8 30% ...

  20. NCBI nr-aa BLAST: CBRC-DNOV-01-2234 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-2234 ref|YP_706575.1| translation initiation factor IF-2 [Rhodococcus ...sp. RHA1] gb|ABG98417.1| translation initiation factor IF-2 [Rhodococcus sp. RHA1] YP_706575.1 0.001 32% ...

  1. NCBI nr-aa BLAST: CBRC-ACAR-01-1085 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-1085 ref|YP_701118.1| ubiquinol-cytochrome c reductase cytochrome b subunit [Rhodococcus... sp. RHA1] gb|ABG92960.1| ubiquinol-cytochrome c reductase cytochrome b subunit [Rhodococcus sp. RHA1] YP_701118.1 1.8 32% ...

  2. NCBI nr-aa BLAST: CBRC-TNIG-22-0127 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-22-0127 ref|YP_705424.1| hypothetical protein RHA1_ro05486 [Rhodococcus s...p. RHA1] gb|ABG97266.1| conserved hypothetical protein [Rhodococcus sp. RHA1] YP_705424.1 1e-08 32% ...

  3. NCBI nr-aa BLAST: CBRC-RNOR-12-0032 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-12-0032 ref|YP_701055.1| possible permease, RarD family [Rhodococcus sp. ...RHA1] gb|ABG92897.1| possible permease, RarD family protein [Rhodococcus sp. RHA1] YP_701055.1 0.99 25% ...

  4. NCBI nr-aa BLAST: CBRC-CJAC-01-1274 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-1274 ref|YP_706575.1| translation initiation factor IF-2 [Rhodococcus ...sp. RHA1] gb|ABG98417.1| translation initiation factor IF-2 [Rhodococcus sp. RHA1] YP_706575.1 8e-11 31% ...

  5. NCBI nr-aa BLAST: CBRC-PTRO-23-0004 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PTRO-23-0004 ref|YP_705424.1| hypothetical protein RHA1_ro05486 [Rhodococcus s...p. RHA1] gb|ABG97266.1| conserved hypothetical protein [Rhodococcus sp. RHA1] YP_705424.1 6e-24 29% ...

  6. NCBI nr-aa BLAST: CBRC-XTRO-01-3892 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-3892 ref|YP_705424.1| hypothetical protein RHA1_ro05486 [Rhodococcus s...p. RHA1] gb|ABG97266.1| conserved hypothetical protein [Rhodococcus sp. RHA1] YP_705424.1 1e-07 37% ...

  7. NCBI nr-aa BLAST: CBRC-XTRO-01-0547 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0547 ref|YP_702726.1| hypothetical protein RHA1_ro02763 [Rhodococcus s...p. RHA1] gb|ABG94568.1| conserved hypothetical protein [Rhodococcus sp. RHA1] YP_702726.1 0.52 33% ...

  8. NCBI nr-aa BLAST: CBRC-DYAK-03-0091 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DYAK-03-0091 ref|YP_707010.1| multidrug resistance transporter, MFS superfamily [Rhodococcus... sp. RHA1] gb|ABG98852.1| multidrug resistance transporter, MFS superfamily protein [Rhodococcus sp. RHA1] YP_707010.1 0.58 39% ...

  9. NCBI nr-aa BLAST: CBRC-ACAR-01-0906 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-0906 ref|YP_708989.1| hypothetical protein RHA1_ro11184 [Rhodococcus s...p. RHA1] gb|ABH00831.1| conserved hypothetical protein [Rhodococcus sp. RHA1] YP_708989.1 2.2 32% ...

  10. NCBI nr-aa BLAST: CBRC-CREM-01-1329 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CREM-01-1329 ref|YP_706733.1| superfamily II DNA and RNA helicases [Rhodococcus... sp. RHA1] gb|ABG98575.1| superfamily II DNA and RNA helicases [Rhodococcus sp. RHA1] YP_706733.1 2e-57 60% ...

  11. NCBI nr-aa BLAST: CBRC-TGUT-16-0000 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TGUT-16-0000 ref|YP_708403.1| hypothetical protein RHA1_ro10052 [Rhodococcus s...p. RHA1] gb|ABH00245.1| conserved hypothetical protein [Rhodococcus sp. RHA1] YP_708403.1 3e-08 29% ...

  12. NCBI nr-aa BLAST: CBRC-CREM-01-1348 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CREM-01-1348 ref|YP_704234.1| hypothetical protein RHA1_ro04285 [Rhodococcus s...p. RHA1] gb|ABG96076.1| conserved hypothetical protein [Rhodococcus sp. RHA1] YP_704234.1 4e-74 41% ...

  13. NCBI nr-aa BLAST: CBRC-ACAR-01-0020 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-0020 ref|YP_706966.1| hypothetical protein RHA1_ro07042 [Rhodococcus s...p. RHA1] gb|ABG98808.1| conserved hypothetical protein [Rhodococcus sp. RHA1] YP_706966.1 7e-17 32% ...

  14. NCBI nr-aa BLAST: CBRC-LAFR-01-1399 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-1399 ref|YP_704314.1| hypothetical protein RHA1_ro04370 [Rhodococcus s...p. RHA1] gb|ABG96156.1| conserved hypothetical protein [Rhodococcus sp. RHA1] YP_704314.1 4.2 30% ...

  15. NCBI nr-aa BLAST: CBRC-PTRO-20-0007 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PTRO-20-0007 ref|YP_700957.1| hypothetical protein RHA1_ro00971 [Rhodococcus s...p. RHA1] gb|ABG92799.1| conserved hypothetical protein [Rhodococcus sp. RHA1] YP_700957.1 6e-05 27% ...

  16. NCBI nr-aa BLAST: CBRC-LAFR-01-0356 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-0356 ref|YP_704314.1| hypothetical protein RHA1_ro04370 [Rhodococcus s...p. RHA1] gb|ABG96156.1| conserved hypothetical protein [Rhodococcus sp. RHA1] YP_704314.1 0.74 33% ...

  17. NCBI nr-aa BLAST: CBRC-ETEL-01-0512 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ETEL-01-0512 ref|YP_706622.1| possible transcriptional regulator [Rhodococcus ...sp. RHA1] gb|ABG98464.1| possible transcriptional regulator [Rhodococcus sp. RHA1] YP_706622.1 2.5 35% ...

  18. NCBI nr-aa BLAST: CBRC-LAFR-01-2827 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-2827 ref|YP_001228699.1| Organic radical activating enzyme [Synechococ...cus sp. RCC307] emb|CAK29346.1| Organic radical activating enzyme [Synechococcus sp. RCC307] YP_001228699.1 6.7 28% ...

  19. NCBI nr-aa BLAST: CBRC-OGAR-01-1052 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OGAR-01-1052 ref|YP_001274802.1| regulator of chromosome condensation, RCC1 [Roseiflexus... sp. RS-1] gb|ABQ88852.1| regulator of chromosome condensation, RCC1 [Roseiflexus sp. RS-1] YP_001274802.1 8e-05 25% ...

  20. NCBI nr-aa BLAST: CBRC-XTRO-01-2385 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-2385 ref|YP_001278902.1| Integrin alpha beta-propellor repeat protein [Roseiflexus... sp. RS-1] gb|ABQ92952.1| Integrin alpha beta-propellor repeat protein [Roseiflexus sp. RS-1] YP_001278902.1 1e-13 37% ...

  1. NCBI nr-aa BLAST: CBRC-GGOR-01-0094 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GGOR-01-0094 ref|YP_001274990.1| hypothetical protein RoseRS_0618 [Roseiflexus... sp. RS-1] gb|ABQ89040.1| hypothetical protein RoseRS_0618 [Roseiflexus sp. RS-1] YP_001274990.1 5.4 28% ...

  2. NCBI nr-aa BLAST: CBRC-PHAM-01-1483 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PHAM-01-1483 ref|YP_002571757.1| laminin G, sub domain 2 [Chloroflexus sp. Y-4...00-fl] gb|ACM55431.1| laminin G, sub domain 2 [Chloroflexus sp. Y-400-fl] YP_002571757.1 5e-45 34% ...

  3. NCBI nr-aa BLAST: CBRC-LAFR-01-2095 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-2095 ref|YP_001274825.1| hypothetical protein RoseRS_0450 [Roseiflexus... sp. RS-1] gb|ABQ88875.1| hypothetical protein RoseRS_0450 [Roseiflexus sp. RS-1] YP_001274825.1 2.9 26% ...

  4. NCBI nr-aa BLAST: CBRC-MMUR-01-1587 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUR-01-1587 ref|YP_001275027.1| hypothetical protein RoseRS_0658 [Roseiflexus... sp. RS-1] gb|ABQ89077.1| hypothetical protein RoseRS_0658 [Roseiflexus sp. RS-1] YP_001275027.1 3e-04 24% ...

  5. NCBI nr-aa BLAST: CBRC-HSAP-20-0012 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-HSAP-20-0012 ref|YP_001277316.1| hypothetical protein RoseRS_3002 [Roseiflexus... sp. RS-1] gb|ABQ91366.1| hypothetical protein RoseRS_3002 [Roseiflexus sp. RS-1] YP_001277316.1 1.0 23% ...

  6. NCBI nr-aa BLAST: CBRC-AGAM-02-0067 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-02-0067 ref|YP_001276495.1| VWA containing CoxE family protein [Roseiflexus... sp. RS-1] gb|ABQ90545.1| VWA containing CoxE family protein [Roseiflexus sp. RS-1] YP_001276495.1 3.1 23% ...

  7. NCBI nr-aa BLAST: CBRC-PMAR-01-0090 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PMAR-01-0090 ref|YP_001277704.1| 2-oxoglutarate dehydrogenase, E1 subunit [Roseiflexus... sp. RS-1] gb|ABQ91754.1| 2-oxoglutarate dehydrogenase, E1 subunit [Roseiflexus sp. RS-1] YP_001277704.1 0.030 36% ...

  8. NCBI nr-aa BLAST: CBRC-TTRU-01-0866 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0866 ref|YP_002571757.1| laminin G, sub domain 2 [Chloroflexus sp. Y-4...00-fl] gb|ACM55431.1| laminin G, sub domain 2 [Chloroflexus sp. Y-400-fl] YP_002571757.1 3e-68 57% ...

  9. NCBI nr-aa BLAST: CBRC-OANA-01-0701 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OANA-01-0701 ref|YP_001276233.1| hypothetical protein RoseRS_1894 [Roseiflexus... sp. RS-1] gb|ABQ90283.1| hypothetical protein RoseRS_1894 [Roseiflexus sp. RS-1] YP_001276233.1 6.8 30% ...

  10. NCBI nr-aa BLAST: CBRC-PHAM-01-0502 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PHAM-01-0502 ref|YP_002571757.1| laminin G, sub domain 2 [Chloroflexus sp. Y-4...00-fl] gb|ACM55431.1| laminin G, sub domain 2 [Chloroflexus sp. Y-400-fl] YP_002571757.1 4e-96 52% ...

  11. NCBI nr-aa BLAST: CBRC-MMUR-01-1073 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUR-01-1073 ref|YP_002570960.1| hypothetical protein Chy400_3256 [Chloroflexus... sp. Y-400-fl] gb|ACM54634.1| hypothetical protein Chy400_3256 [Chloroflexus sp. Y-400-fl] YP_002570960.1 0.003 28% ...

  12. NCBI nr-aa BLAST: CBRC-DNOV-01-1573 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-1573 ref|YP_001276270.1| alpha amylase, catalytic region [Roseiflexus ...sp. RS-1] gb|ABQ90320.1| alpha amylase, catalytic region [Roseiflexus sp. RS-1] YP_001276270.1 1.7 37% ...

  13. NCBI nr-aa BLAST: CBRC-CREM-01-1328 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CREM-01-1328 ref|YP_045146.1| pH adaptation potassium efflux system transmembr...ane protein [Acinetobacter sp. ADP1] emb|CAG67324.1| pH adaptation potassium efflux system transmembrane protein [Acinetobacter sp. ADP1] YP_045146.1 1e-133 85% ...

  14. NCBI nr-aa BLAST: CBRC-XTRO-01-2152 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-2152 ref|YP_372796.1| cytosine/purines, uracil, thiamine, allantoin tr...ansporter [Burkholderia sp. 383] gb|ABB12152.1| cytosine/purines, uracil, thiamine, allantoin transporter [Burkholderia sp. 383] YP_372796.1 0.12 32% ...

  15. NCBI nr-aa BLAST: CBRC-DNOV-01-1580 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-1580 ref|ZP_01306680.1| sodium-dependent transporter family protein [Ocean...obacter sp. RED65] gb|EAT12695.1| sodium-dependent transporter family protein [Oceanobacter sp. RED65] ZP_01306680.1 0.45 28% ...

  16. NCBI nr-aa BLAST: CBRC-PCAP-01-0207 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PCAP-01-0207 ref|ZP_01168353.1| hypothetical protein MED92_11654 [Oceanospiril...lum sp. MED92] gb|EAR59570.1| hypothetical protein MED92_11654 [Oceanospirillum sp. MED92] ZP_01168353.1 0.94 28% ...

  17. NCBI nr-aa BLAST: CBRC-DMEL-03-0007 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DMEL-03-0007 ref|ZP_01827442.1| ribosome recycling factor [Streptococcus pneum...oniae SP14-BS69] gb|EDK66437.1| ribosome recycling factor [Streptococcus pneumoniae SP14-BS69] ZP_01827442.1 2.2 25% ...

  18. NCBI nr-aa BLAST: CBRC-DMEL-03-0007 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DMEL-03-0007 ref|ZP_01834868.1| ribosome recycling factor [Streptococcus pneum...oniae SP23-BS72] gb|EDK81904.1| ribosome recycling factor [Streptococcus pneumoniae SP23-BS72] ZP_01834868.1 2.2 25% ...

  19. NCBI nr-aa BLAST: CBRC-MDOM-03-0048 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MDOM-03-0048 ref|ZP_01884120.1| Mn2+/Fe2+ transporter, NRAMP family protein [Pedo...bacter sp. BAL39] gb|EDM36559.1| Mn2+/Fe2+ transporter, NRAMP family protein [Pedobacter sp. BAL39] ZP_01884120.1 3.2 26% ...

  20. NCBI nr-aa BLAST: CBRC-MEUG-01-0725 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MEUG-01-0725 ref|ZP_01886730.1| rod shape-determining protein [Pedobacter sp. ...BAL39] gb|EDM34064.1| rod shape-determining protein [Pedobacter sp. BAL39] ZP_01886730.1 3.8 26% ...

  1. NCBI nr-aa BLAST: CBRC-BTAU-01-1375 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-BTAU-01-1375 ref|ZP_01886595.1| K+-transporting ATPase B chain [Pedobacter sp.... BAL39] gb|EDM34188.1| K+-transporting ATPase B chain [Pedobacter sp. BAL39] ZP_01886595.1 2.2 27% ...

  2. NCBI nr-aa BLAST: CBRC-EEUR-01-0940 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-EEUR-01-0940 ref|ZP_01885742.1| putative outer membrane transport/efflux protein [Pedo...bacter sp. BAL39] gb|EDM35039.1| putative outer membrane transport/efflux protein [Pedobacter sp. BAL39] ZP_01885742.1 6.7 43% ...

  3. NCBI nr-aa BLAST: CBRC-AGAM-07-0044 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-07-0044 ref|ZP_01884120.1| Mn2+/Fe2+ transporter, NRAMP family protein [Pedo...bacter sp. BAL39] gb|EDM36559.1| Mn2+/Fe2+ transporter, NRAMP family protein [Pedobacter sp. BAL39] ZP_01884120.1 1e-119 55% ...

  4. NCBI nr-aa BLAST: CBRC-RNOR-03-0426 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-03-0426 ref|ZP_01885543.1| undecaprenyl-phosphomannose:protein mannosyltransferase [Pedo...bacter sp. BAL39] gb|EDM35326.1| undecaprenyl-phosphomannose:protein mannosyltransferase [Pedobacter sp. BAL39] ZP_01885543.1 0.31 24% ...

  5. NCBI nr-aa BLAST: CBRC-AGAM-07-0040 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-07-0040 ref|ZP_01882627.1| multidrug resistance protein [Pedobacter sp. B...AL39] gb|EDM38378.1| multidrug resistance protein [Pedobacter sp. BAL39] ZP_01882627.1 3e-50 42% ...

  6. NCBI nr-aa BLAST: CBRC-DDIS-02-0191 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DDIS-02-0191 ref|ZP_01884120.1| Mn2+/Fe2+ transporter, NRAMP family protein [Pedo...bacter sp. BAL39] gb|EDM36559.1| Mn2+/Fe2+ transporter, NRAMP family protein [Pedobacter sp. BAL39] ZP_01884120.1 1e-71 50% ...

  7. NCBI nr-aa BLAST: CBRC-AGAM-07-0073 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-07-0073 ref|ZP_01886744.1| hypothetical protein PBAL39_17149 [Pedobacter ...sp. BAL39] gb|EDM34019.1| hypothetical protein PBAL39_17149 [Pedobacter sp. BAL39] ZP_01886744.1 1e-114 75% ...

  8. NCBI nr-aa BLAST: CBRC-XTRO-01-0587 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0587 ref|ZP_01883526.1| CHU large protein; uncharacterized [Pedobacter... sp. BAL39] gb|EDM36996.1| CHU large protein; uncharacterized [Pedobacter sp. BAL39] ZP_01883526.1 0.077 20% ...

  9. NCBI nr-aa BLAST: CBRC-TNIG-22-0185 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TNIG-22-0185 ref|ZP_01047391.1| putative manganese transport protein MntH [Nit...robacter sp. Nb-311A] gb|EAQ34662.1| putative manganese transport protein MntH [Nitrobacter sp. Nb-311A] ZP_01047391.1 0.11 28% ...

  10. NCBI nr-aa BLAST: CBRC-DDIS-02-0191 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DDIS-02-0191 ref|ZP_01302230.1| putative manganese transport protein MntH [Sph...ingomonas sp. SKA58] gb|EAT10059.1| putative manganese transport protein MntH [Sphingomonas sp. SKA58] ZP_01302230.1 1e-71 50% ...

  11. NCBI nr-aa BLAST: CBRC-AGAM-07-0044 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-07-0044 ref|ZP_01721553.1| manganese transport protein MntH [Algoriphagus... sp. PR1] gb|EAZ79142.1| manganese transport protein MntH [Algoriphagus sp. PR1] ZP_01721553.1 1e-104 62% ...

  12. NCBI nr-aa BLAST: CBRC-AGAM-07-0044 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-07-0044 ref|ZP_01302230.1| putative manganese transport protein MntH [Sph...ingomonas sp. SKA58] gb|EAT10059.1| putative manganese transport protein MntH [Sphingomonas sp. SKA58] ZP_01302230.1 1e-100 52% ...

  13. NCBI nr-aa BLAST: CBRC-DSIM-03-0061 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DSIM-03-0061 ref|ZP_01725160.1| Transporter, Drug/Metabolite Exporter family p...rotein [Bacillus sp. B14905] gb|EAZ84362.1| Transporter, Drug/Metabolite Exporter family protein [Bacillus sp. B14905] ZP_01725160.1 0.81 30% ...

  14. NCBI nr-aa BLAST: CBRC-XTRO-01-0078 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0078 ref|ZP_01165553.1| cobalamin biosynthesis protein CbiM [Oceanospi...rillum sp. MED92] gb|EAR62331.1| cobalamin biosynthesis protein CbiM [Oceanospirillum sp. MED92] ZP_01165553.1 2e-64 66% ...

  15. NCBI nr-aa BLAST: CBRC-CREM-01-1301 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CREM-01-1301 ref|ZP_01168030.1| Na+/H+ antiporter, putative [Oceanospirillum s...p. MED92] gb|EAR59909.1| Na+/H+ antiporter, putative [Oceanospirillum sp. MED92] ZP_01168030.1 3e-81 52% ...

  16. NCBI nr-aa BLAST: CBRC-DNOV-01-3161 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-3161 ref|ZP_01166846.1| probable signalling protein [Oceanospirillum s...p. MED92] gb|EAR61014.1| probable signalling protein [Oceanospirillum sp. MED92] ZP_01166846.1 6.8 33% ...

  17. NCBI nr-aa BLAST: CBRC-DNOV-01-0257 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-0257 ref|ZP_01167931.1| putrescine ABC transporter, permease protein [Ocean...ospirillum sp. MED92] gb|EAR59953.1| putrescine ABC transporter, permease protein [Oceanospirillum sp. MED92] ZP_01167931.1 0.80 39% ...

  18. Pgc-1α and Nr4a1 Are Target Genes of Circadian Melatonin and Dopamine Release in Murine Retina.

    Science.gov (United States)

    Kunst, Stefanie; Wolloscheck, Tanja; Kelleher, Debra K; Wolfrum, Uwe; Sargsyan, S Anna; Iuvone, P Michael; Baba, Kenkichi; Tosini, Gianluca; Spessert, Rainer

    2015-09-01

    The neurohormones melatonin and dopamine mediate clock-dependent/circadian regulation of inner retinal neurons and photoreceptor cells and in this way promote their functional adaptation to time of day and their survival. To fulfill this function they act on melatonin receptor type 1 (MT1 receptors) and dopamine D4 receptors (D4 receptors), respectively. The aim of the present study was to screen transcriptional regulators important for retinal physiology and/or pathology (Dbp, Egr-1, Fos, Nr1d1, Nr2e3, Nr4a1, Pgc-1α, Rorβ) for circadian regulation and dependence on melatonin signaling/MT1 receptors or dopamine signaling/D4 receptors. This was done by gene profiling using quantitative polymerase chain reaction in mice deficient in MT1 or D4 receptors. The data obtained determined Pgc-1α and Nr4a1 as transcriptional targets of circadian melatonin and dopamine signaling, respectively. The results suggest that Pgc-1α and Nr4a1 represent candidate genes for linking circadian neurohormone release with functional adaptation and healthiness of retina and photoreceptor cells.

  19. Diminution of the NMDA receptor NR2B subunit in cortical and subcortical areas of WAG/Rij rats.

    Science.gov (United States)

    Karimzadeh, Fariba; Soleimani, Mansoureh; Mehdizadeh, Mehdi; Jafarian, Maryam; Mohamadpour, Maliheh; Kazemi, Hadi; Joghataei, Mohammad-Taghi; Gorji, Ali

    2013-12-01

    Modulation of glutamatergic NMDA receptors affects the synchronization of spike discharges in in WAG/Rij rats, a valid genetic animal model of absence epilepsy. In this study, we describe the alteration of NR2B subunit of NMDA receptors expression in WAG/Rij rats in different somatosensory cortical layers and in hippocampal CA1 area. Experimental groups were divided into four groups of six rats of both WAG/Rij and Wistar strains with 2 and 6 months of age. The distribution of NR2B receptors was assessed by immunohistochemical staining in WAG/Rij and compared with age-matched Wistar rats. The expression of NR2B subunit was significantly decreased in different somatosensory cortical layers in 2- and 6-month-old WAG/Rij rats. In addition, the distribution of NR2B in hippocampal CA1 area was lower in 6-month-old WAG/Rij compared with age-matched Wistar rats. The reduction of NR2B receptors in different brain areas points to disturbance of glutamate receptors expression in cortical and subcortical areas in WAG/Rij rats. An altered subunit assembly of NMDA receptors may underlie cortical hyperexcitability in absence epilepsy. Copyright © 2013 Wiley Periodicals, Inc.

  20. Penggunaan Jamur Antagonis Trichoderma SP. Dan Gliocladium SP. Untuk Mengendalikan Penyakit Layu Fusarium Pada Tanaman Bawang Merah (Allium Ascalonicum L.)

    OpenAIRE

    Arie Ramadhina, Arie Ramadhina; Lisnawita, Lisnawita; Lubis, Lahmuddin

    2013-01-01

    The use of antagonism fungus of Trichoderma sp. and Gliocladium sp. for controlling wilt(Fusarium oxysporum) in red onion plants. The aim of the research was to know the effectiviness ofantagonism fungus of Trichoderma sp. and Gliocladium sp. in controlling wilt in red onion plants.The research used non-factorial RAK (random group design) with eight treatments: control, 10grams of F. oxysporum, 12 grams of Trichoderma sp., 18 grams of Trichoderma sp., 24 grams ofTrichoderma sp., and 12 grams ...

  1. Chaenorhinum semispeluncarum sp. nov. and C. yildirimlii sp. nov. (Scrophulariaceae) from east Anatolia, Turkey

    DEFF Research Database (Denmark)

    Yildirim, Hasan; Tan, Kit; Senol, Serdar

    2010-01-01

    Chaenorhinum semispeluncarum H. Yildirim, Kit Tan, S. Senol & A. Pirhan sp. nov. and C. yildirimlii Kit Tan, H. Yildirim, S. Senol & A. Pirhan sp. nov. (Scrophulariaceae, C. sect. Microrrhinum) from east Anatolia are described and illustrated. They are both narrow endemics related to the rare C...

  2. Integrative proteomic analysis of the NMDA NR1 knockdown mouse model reveals effects on central and peripheral pathways associated with schizophrenia and autism spectrum disorders

    NARCIS (Netherlands)

    H. Wesseling (Hendrik); P.C. Guest (Paul); C.-M. Lee (Chi-Ming); E.H.F. Wong (Erik); H. Rahmoune (Hassan); S. Bahn (Sabine)

    2014-01-01

    textabstractBackground: Over the last decade, the transgenic N-methyl-D-aspartate receptor (NMDAR) NR1-knockdown mouse (NR1neo-/-) has been investigated as a glutamate hypofunction model for schizophrenia. Recent research has now revealed that the model also recapitulates cognitive and negative

  3. A unique 970kb microdeletion in 9q33.3, including the NR5A1 gene in a 46,XY female

    NARCIS (Netherlands)

    Van Silfhout, Anneke; Boot, Annemieke M.; Dijkhuizen, Trijnie; Hoek, Annemieke; Nijman, Rien; Sikkema-Raddatz, Birgit; van Ravenswaaij-Arts, Conny M. A.

    2009-01-01

    We report on a female patient with XY sex reversal with clitoromegaly, neonatal male testosterone and AMH levels, and a normal urine steroid profile. Array CGH revealed a de novo microdeletion of chromosome 9q33.3, including the NR5A1 gene. NR5A1 encodes for the steroidogenic factor-1 (SF-1) and

  4. Blending of NR/BR/EPDM by reactive processing for tire sidewall applications: Part I - Preparation, cure characteristics and mechanical properties

    NARCIS (Netherlands)

    Sahakaro, Kannika; Naskar, N.; Naskar, Nityananda; Noordermeer, Jacobus W.M.; Datta, Rabin

    2007-01-01

    EPDM incorporated into blends of natural rubber/butadiene rubber (NR/BR) improves ozone resistance. In this work, the inferior mechanical properties of NR/BR/EPDM blends generally obtained by conventional straight mixing are overcome by utilizing a reactive processing technique. The entire amount of

  5. QTL list: Nr Roots 2006 [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

    Lifescience Database Archive (English)

    Full Text Available orrhizal) plant ... P38M48 LG_09 ... 3.79 ... 10.1007/s00122-010-1501-8 21222096 ... QT63208 Allium cepa Amaryllidaceae Nr Roots 2006 Nr Roots Number of stem-borne roots per AM (arbuscular myc

  6. QTL list: Nr Roots 2007 [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

    Lifescience Database Archive (English)

    Full Text Available orrhizal) plant ... P31M33 LG_09 ... 3.74 ... 10.1007/s00122-010-1501-8 21222096 ... QT63209 Allium cepa Amaryllidaceae Nr Roots 2007 Nr Roots Number of stem-borne roots per AM (arbuscular myc

  7. Proposal for inclusion of the risk based inspection technique in Regulatory Standard NR 13; Proposta de inclusao da tecnica de inspecao baseada em risco na Norma Regulamentadora NR 13

    Energy Technology Data Exchange (ETDEWEB)

    Esteves, Vinicius Teixeira; Lima, Marco Aurelio Oliveira [Det Norske Veritas Ltda. (DNV), Rio de Janeiro, RJ (Brazil)

    2012-07-01

    In Brazil, the Regulatory Standard n. 13 (NR 13) establishes requirements for the inspection of boilers and pressure vessels which has main objective of preventing accidents with these types of equipment. Additionally, it has the Risk-Based Inspection (RBI) technique as an effective way to manage the mechanical integrity of various types of static mechanical equipment by through an inspection planning based on the risk factor. In this study, it is being proposed to include the RBI technique, in the NR 13, for the planning and definition of periods for the safety inspection of boilers and pressure vessels in order to promote an increase in the operational safety in process industries in Brazil. In this study it was carried out a critical analysis of NR 13 and RBI, and beyond that a bibliographic research of various international documents that relate the operational safety of pressurized equipment with the inspection activity, and the acceptability of RBI by governments, agencies and organizations around the world. It is considered that the inclusion and formal acceptance of RBI technique in the NR 13 must be accompanied by a rigorous control to avoid the 'trivialization' of its use and ensure the implementation rational, efficient and reliable. Finally, it was developed and suggested basic elements and minimum requirements to be inserted in the NR 13, to be attended, in order mandatory, by the companies that choose the implementation and use of the RBI technique as a tool for the planning of safety inspection of boilers and pressure vessels. It is concluded that the formal acceptance of the RBI technique in the NR 13 could aggregate much value to this standard, with regard to the prevention of accidents involving boilers or pressure vessels, and provide a technological jump to the companies that make use of RBI technique in Brazil. (author)

  8. NR2A and NR2B receptor gene variations modify age at onset in Huntington disease in a sex-specific manner.

    Science.gov (United States)

    Arning, Larissa; Saft, Carsten; Wieczorek, Stefan; Andrich, Jürgen; Kraus, Peter H; Epplen, Jörg T

    2007-09-01

    In addition to the pathogenetic CAG repeat expansion other genetic factors play a significant role in determining age at onset (AO) in Huntington disease (HD), e.g. variations in the NR2A and NR2B glutamate receptor subunit genes (GRIN2A, GRIN2B). In order to expand these findings we fine-mapped a larger HD patient panel (n = 250) using densely spaced markers flanking the originally associated SNPs in GRIN2A and GRIN2B. In GRIN2A association fine-mapping based on eight additional SNPs confirmed intron 2 as the region of strongest association. In GRIN2B fine-mapping with seven additional SNPs consolidated C2664T as causal genetic variation. Gender stratification of patients revealed differences in the variability in AO attributable to the CAG repeat number and highly significant differences in the AO association with the C2664T and rs8057394/ rs2650427 variations. Addition of the corresponding genotype variations to the effect of CAG repeat lengths resulted in a significant increase of the R2 values only in females. The sex-specific effect for C2664T is underscored by differences in the genotype and allele frequencies observed for female versus male HD patients (P = 0.01) caused by decreased CC frequency in females. Overall, female HD patients homozygous for the CC genotype tended to have later AO compared to the other two genotypes. Stratification of the results by presumed menopausal status demonstrated that the significant findings were predominantly observed in pre-menopausal patients. We speculate that altered hormone levels herald protective effects of this genotype. Together, GRIN2A and GRIN2B genotype variations explain 7.2% additional variance in AO for HD.

  9. NR2A-Containing NMDARs in the Prefrontal Cortex Are Required for Working Memory and Associated with Age-Related Cognitive Decline.

    Science.gov (United States)

    McQuail, Joseph A; Beas, B Sofia; Kelly, Kyle B; Simpson, Kailey L; Frazier, Charles J; Setlow, Barry; Bizon, Jennifer L

    2016-12-14

    Working memory, the ability to temporarily maintain representational knowledge, is a foundational cognitive process that can become compromised in aging and neuropsychiatric disease. NMDA receptor (NMDAR) activation in prefrontal cortex (PFC) is necessary for the pyramidal neuron activity believed to enable working memory; however, the distinct biophysical properties and localization of NMDARs containing NR2A and NR2B subunits suggest unique roles for NMDAR subtypes in PFC neural activity and working memory. Experiments herein show that working memory depends on NR2A- but not NR2B-NMDARs in PFC of rats and that NR2A-NMDARs mediate the majority of evoked NMDAR currents on layer 2/3 PFC pyramidal neurons. Moreover, attenuated expression of the NR2A but not the NR2B subunit in PFC associates with naturally occurring working memory impairment in aged rats. Finally, NMDAR currents and working memory are enhanced in aged rats by promoting activation of the NR2A-enriched synaptic pool of PFC NMDARs. These results implicate NR2A-NMDARs in normal working memory and suggest novel treatment strategies for improving working memory in cognitive disorders. Working memory, the ability to hold information "in mind," requires persistent activity of pyramidal neurons in prefrontal cortex (PFC) mediated by NMDA receptor (NMDAR) activation. NMDAR loss in PFC may account for working memory impairments in aging and psychiatric disease. Our studies demonstrate that NMDARs containing the NR2A subunit, but not the NR2B subunit, are required for working memory and that loss of NR2A predicts severity of age-related working memory impairment. The importance of NR2A to working memory is likely due its abundant contribution to pyramidal neuron activity and location at synaptic sites in PFC. This information is useful in designing new therapies to treat working memory impairments by enhancing the function of NR2A-containing NMDARs. Copyright © 2016 the authors 0270-6474/16/3612537-12$15.00/0.

  10. Hyperactivity, startle reactivity and cell-proliferation deficits are resistant to chronic lithium treatment in adult Nr2e1(frc/frc) mice.

    Science.gov (United States)

    Wong, B K Y; Hossain, S M; Trinh, E; Ottmann, G A; Budaghzadeh, S; Zheng, Q Y; Simpson, E M

    2010-10-01

    The NR2E1 region on Chromosome 6q21-22 has been repeatedly linked to bipolar disorder (BP) and NR2E1 has been associated with BP, and more specifically bipolar I disorder (BPI). In addition, patient sequencing has shown an enrichment of rare candidate-regulatory variants. Interestingly, mice carrying either spontaneous (Nr2e1(frc) ) or targeted (Tlx(-) ) deletions of Nr2e1 (here collectively known as Nr2e1-null) show similar neurological and behavioral anomalies, including hypoplasia of the cerebrum, reduced neural stem cell proliferation, extreme aggression and deficits in fear conditioning; these are the traits that have been observed in some patients with BP. Thus, NR2E1 is a positional and functional candidate for a role in BP. However, no Nr2e1-null mice have been fully evaluated for behaviors used to model BP in rodents or pharmacological responses to drugs effective in treating BP symptoms. In this study we examine Nr2e1(frc/frc) mice, homozygous for the spontaneous deletion, for abnormalities in activity, learning and information processing, and cell proliferation; these are the phenotypes that are either affected in patients with BP or commonly assessed in rodent models of BP. The effect of lithium, a drug used to treat BP, was also evaluated for its ability to attenuate Nr2e1(frc/frc) behavioral and neural stem cell-proliferation phenotypes. We show for the first time that Nr2e1-null mice exhibit extreme hyperactivity in the open field as early as postnatal day 18 and in the home cage, deficits in open-field habituation and passive avoidance, and surprisingly, an absence of acoustic startle. We observed a reduction in neural stem/progenitor cell proliferation in Nr2e1(frc/frc) mice, similar to that seen in other Nr2e1-null strains. These behavioral and cell-proliferation phenotypes were resistant to chronic-adult-lithium treatment. Thus, Nr2e1(frc/frc) mice exhibit behavioral traits used to model BP in rodents, but our results do not support Nr2e1(frc

  11. Avaliação do cumprimento da NR-18 em função do porte de obra residencial e proposta de lista de verificação da NR-18

    OpenAIRE

    Costella, Marcelo Fabiano; Junges, Franciele Cristina; Pilz, Silvio Edmundo

    2014-01-01

    O presente trabalho investiga as reais condições dos ambientes de trabalho da indústria da construção, tendo em vista os problemas relacionados à segurança do trabalho nos canteiros de obras. Formulou-se a hipótese de haver discrepância no grau de cumprimento da NR-18 em obras de diferentes portes (pequena, média e grande). Baseado em uma lista de verificação do grau de cumprimento dos requisitos da NR-18, foram avaliados 115 canteiros de obra na cidade de Chapecó - SC. Para cada item da list...

  12. Influence of different curing systems on the physico-mechanical properties and stability of SBR and NR rubbers

    CERN Document Server

    Basfar, A A; Mofti, S

    2002-01-01

    The physical properties of radiation, sulfur and peroxide-cured styrene-butadiene rubber (SBR) and natural rubber (NR) were compared. The dependence of the mechanical properties of the radiation-vulcanized SBR and NR on the coagent concentration and radiation dose was studied. The effect of thermal aging on the mechanical properties of the different rubber formulations was discussed. The radiation-cured formulations of SBR have superior mechanical properties and thermal stability compared with those of the chemically vulcanized compounds. Whereas, the radiation-cured formulations of NR have similar mechanical properties but superior thermal stability (based on the % change in E after thermal aging), when compared with those of the sulfur-vulcanized compounds and slightly better than those of the peroxide-vulcanized compounds.

  13. Metallaphotoredox-catalysed sp3-sp3 cross-coupling of carboxylic acids with alkyl halides

    Science.gov (United States)

    Johnston, Craig P.; Smith, Russell T.; Allmendinger, Simon; MacMillan, David W. C.

    2016-08-01

    In the past 50 years, cross-coupling reactions mediated by transition metals have changed the way in which complex organic molecules are synthesized. The predictable and chemoselective nature of these transformations has led to their widespread adoption across many areas of chemical research. However, the construction of a bond between two sp3-hybridized carbon atoms, a fundamental unit of organic chemistry, remains an important yet elusive objective for engineering cross-coupling reactions. In comparison to related procedures with sp2-hybridized species, the development of methods for sp3-sp3 bond formation via transition metal catalysis has been hampered historically by deleterious side-reactions, such as β-hydride elimination with palladium catalysis or the reluctance of alkyl halides to undergo oxidative addition. To address this issue, nickel-catalysed cross-coupling processes can be used to form sp3-sp3 bonds that utilize organometallic nucleophiles and alkyl electrophiles. In particular, the coupling of alkyl halides with pre-generated organozinc, Grignard and organoborane species has been used to furnish diverse molecular structures. However, the manipulations required to produce these activated structures is inefficient, leading to poor step- and atom-economies. Moreover, the operational difficulties associated with making and using these reactive coupling partners, and preserving them through a synthetic sequence, has hindered their widespread adoption. A generically useful sp3-sp3 coupling technology that uses bench-stable, native organic functional groups, without the need for pre-functionalization or substrate derivatization, would therefore be valuable. Here we demonstrate that the synergistic merger of photoredox and nickel catalysis enables the direct formation of sp3-sp3 bonds using only simple carboxylic acids and alkyl halides as the nucleophilic and electrophilic coupling partners, respectively. This metallaphotoredox protocol is suitable for

  14. The nuclear receptor NR4A1 induces a form of cell death dependent on autophagy in mammalian cells.

    Directory of Open Access Journals (Sweden)

    Jimena Bouzas-Rodríguez

    Full Text Available The control of cell death is a biological process essential for proper development, and for preventing devastating pathologies like cancer and neurodegeneration. On the other hand, autophagy regulation is essential for protein and organelle degradation, and its dysfunction is associated with overlapping pathologies like cancer and neurodegeneration, but also for microbial infection and aging. In the present report we show that two evolutionarily unrelated receptors--Neurokinin 1 Receptor (NK(1R, a G-protein coupled receptor, and Insulin-like Growth Factor 1 Receptor (IGF1R, a tyrosine kinase receptor--both induce non-apoptotic cell death with autophagic features and requiring the activity of the autophagic core machinery proteins PI3K-III, Beclin-1 and Atg7. Remarkably, this form of cell death occurs in apoptosis-competent cells. The signal transduction pathways engaged by these receptors both converged on the activation of the nuclear receptor NR4A1, which has previously been shown to play a critical role in some paradigms of apoptosis and in NK(1R-induced cell death. The activity of NR4A1 was necessary for IGF1R-induced cell death, as well as for a canonical model of cell death by autophagy induced by the presence of a pan-caspase inhibitor, suggesting that NR4A1 is a general modulator of this kind of cell death. During cell death by autophagy, NR4A1 was transcriptionally competent, even though a fraction of it was present in the cytoplasm. Interestingly, NR4A1 interacts with the tumor suppressor p53 but not with Beclin-1 complex. Therefore the mechanism to promote cell death by autophagy might involve regulation of gene expression, as well as protein interactions. Understanding the molecular basis of autophagy and cell death mediation by NR4A1, should provide novel insights and targets for therapeutic intervention.

  15. Caffeine increases Nr1i3 expression and potentiates the effects of its ligand, TCPOBOP, in mice liver

    Directory of Open Access Journals (Sweden)

    Heidge Fukumasu

    2015-06-01

    Full Text Available Caffeine is one of the world's most consumed substances. It is present in coffee, green tea and guarana, among others. The xenobiotic-sensing nuclear receptor subfamily 1, group I, member 3 (Nr1i3, also known as the Constitutive Androstane Receptor (Car is a key regulator of drug metabolism and excretion. No consistent description of caffeine effects on this receptor has been described. Thus, to unravel the effects of caffeine on this receptor, we performed experiments in mice. First, C57Bl/6 mice that were treated daily with caffeine (50 mg/kg for 15 days presented a slight but significant increase in Nr1i3 and Cyp2b10 gene expression. A second experiment was then performed to verify the effects of caffeine on TCPOBOP (1,4-bis-[2-(3,5-dichloropyridyloxy]benzene, 3,3′,5,5′-tetrachloro-1,4-bis(pyridyloxybenzene, the most potent agonist known for mice Nr1i3. Interestingly, caffeine potentiated TCPOBOP pleiotropic effects in mice liver, such as hepatomegaly, hepatotoxicity, hepatocyte proliferation and loss of cell-to-cell communication through gap junctions. In addition, caffeine plus TCPOBOP treatment increased liver gene expression of Nr1i3 and Cyp2b10 comparing with only caffeine or TCPOBOP treatments. Together, these results indicate that caffeine increases the expression of Nr1i3 in mice liver, although at this point it is not possible to determine if Nr1i3 directly or indirectly mediates this effect.

  16. Five novel Wickerhamomyces- and Metschnikowia-related yeast species, Wickerhamomyces chaumierensis sp. nov., Candida pseudoflosculorum sp. nov., Candida danieliae sp. nov., Candida robnettiae sp. nov. and Candida eppingiae sp. nov., isolated from plants

    NARCIS (Netherlands)

    Groenewald, Marizeth; Robert, Vincent; Smith, Maudy Th

    On the basis of nucleotide divergences in the D1/D2 domain of the 26S rRNA gene and the internal transcribed spacers (ITS) domain of the rRNA gene, five novel yeast species, Wickerhamomyces chaumierensis sp. nov. (CBS 8565(T)  = JCM 17246(T)), Candida pseudoflosculorum sp. nov. (CBS 8584(T)  = JCM

  17. Pacific Northwest National Laboratory Apatite Investigation at the 100-NR-2 Quality Assurance Project Plan

    Energy Technology Data Exchange (ETDEWEB)

    Fix, N. J.

    2008-03-28

    This Quality Assurance Project Plan provides the quality assurance requirements and processes that will be followed by staff working on the 100-NR-2 Apatite Project. The U.S. Department of Energy, Fluor Hanford, Inc., Pacific Northwest National Laboratory, and the Washington Department of Ecology agreed that the long-term strategy for groundwater remediation at 100-N would include apatite sequestration as the primary treatment, followed by a secondary treatment. The scope of this project covers the technical support needed before, during, and after treatment of the targeted subsurface environment using a new high-concentration formulation.

  18. Investigation of associations between NR1D1, RORA and RORB genes and bipolar disorder.

    Directory of Open Access Journals (Sweden)

    Yin-Chieh Lai

    Full Text Available Several genes that are involved in the regulation of circadian rhythms are implicated in the susceptibility to bipolar disorder (BD. The current study aimed to investigate the relationships between genetic variants in NR1D1 RORA, and RORB genes and BD in the Han Chinese population. We conducted a case-control genetic association study with two samples of BD patients and healthy controls. Sample I consisted of 280 BD patients and 200 controls. Sample II consisted of 448 BD patients and 1770 healthy controls. 27 single nucleotide polymorphisms in the NR1D1, RORA, and RORB genes were genotyped using GoldenGate VeraCode assays in sample I, and 492 markers in the three genes were genotyped using Affymetrix Genome-Wide CHB Array in sample II. Single marker and gene-based association analyses were performed using PLINK. A combined p-value for the joining effects of all markers within a gene was calculated using the rank truncated product method. Multifactor dimensionality reduction (MDR method was also applied to test gene-gene interactions in sample I. All markers were in Hardy-Weinberg equilibrium (P>0.001. In sample I, the associations with BD were observed for rs4774388 in RORA (OR = 1.53, empirical p-value, P = 0.024, and rs1327836 in RORB (OR = 1.75, P = 0.003. In Sample II, there were 45 SNPs showed associations with BD, and the most significant marker in RORA was rs11639084 (OR = 0.69, P = 0.002, and in RORB was rs17611535 (OR = 3.15, P = 0.027. A combined p-value of 1.6×10-6, 0.7, and 1.0 was obtained for RORA, RORB and NR1D1, respectively, indicting a strong association for RORA with the risk of developing BD. A four way interaction was found among markers in NR1D1, RORA, and RORB with the testing accuracy 53.25% and a cross-validation consistency of 8 out of 10. In sample II, 45 markers had empirical p-values less than 0.05. The most significant markers in RORA and RORB genes were rs11639084 (OR = 0.69, P = 0.002, and rs17611535 (OR = 3

  19. SP-100/Brayton power system concepts

    International Nuclear Information System (INIS)

    Owen, D.F.

    1989-01-01

    Use of closed Brayton cycle (CBC) power conversion technology has been investigated for use with SP-100 reactors for space power systems. The CBC power conversion technology is being developed by Rockwell International under the Dynamic Isotype Power System (DIPS) and Space Station Freedom solar dynamic power system programs to provide highly efficient power conversion with radioisotype and solar collector heat sources. Characteristics including mass, radiator area, thermal power, and operating temperatures for systems utilizing SP-100 reactor and CBC power conversion technology were determined for systems in the 10-to 100-kWe power range. Possible SP-100 reactor/CBC power system configurations are presented. Advantages of CBC power conversion technology with regard to reactor thermal power, operating temperature, and development status are discussed

  20. Ovos de Toxocara sp. e larvas de Ancylostoma sp. em praça pública de Lavras, MG Toxocara sp. eggs and Ancylostoma sp. larva in public parks, Brazil

    Directory of Open Access Journals (Sweden)

    Antônio Marcos Guimarães

    2005-04-01

    Full Text Available Larva migrans visceral e cutânea são zoonoses parasitárias causadas pela infecção da larva de Toxocara sp. e Ancylostoma sp., respectivamente. O objetivo do estudo foi verificar a contaminação por ovos de Toxocara sp. e ovos e larvas de Ancylostoma sp. em amostras de solos coletadas de praças públicas e de áreas de recreação infantil de Lavras, Estado de Minas Gerais, por meio da técnica de centrífugo-flutuação e do método de Baermann. A ocorrência de ovos de Toxocara sp. e, ovos e larvas de Ancylostoma sp. foi observada em 69,6% (16/23 das amostras de solo coletadas de praças públicas. A contaminação somente por ovos de Ancylostoma sp. em amostras de solo coletadas em escolas/creches foi de 22,2% (4/18. A percentagem de amostras de areia coletadas de escolas/creches contaminadas somente com larvas de Ancylostoma sp. foi de 11,1% (2/18. Praças públicas são as áreas com maior risco potencial de infecção por Toxocara sp. e Ancylostoma sp. Exame coproparasitológico realizado em 174 amostras de fezes de cães observou 58% e 23%, respectivamente, com ovos de Ancylostoma sp. e Toxocara sp.Visceral and cutaneous larva migrans are parasitic zoonoses caused by the infection of larval nematodes Toxocara sp. and Ancylostoma sp. respectively. The objective of this study was to investigate the contamination by Toxocara sp. eggs and Ancylostoma sp. eggs and larva of soil samples collected from public parks and children's playground areas in state of Minas Gerais, Brazil, using both Baermann's method and centrifugal flotation technique. Toxocara sp. and Ancylostoma sp. eggs were observed in soil samples collected from public squares in 17.4% (4/23 and 69.6 (16/23 respectively. In schools and child day care settings the contamination by Ancylostoma sp. larva in sand samples was 11.1% (2/18. Public parks are settings of more potential risk of Toxocara sp. eggs and Ancylostoma sp. infection. Stool parasitology testing of 174 stool

  1. Anti-Inflammatory Activity of Polysaccharide Fraction of Curcuma longa Extract (NR-INF-02).

    Science.gov (United States)

    Illuri, Ramanaiah; Bethapudi, Bharathi; Anandakumar, Senthilkumar; Murugan, Sasikumar; Joseph, Joshua A; Mundkinajeddu, Deepak; Agarwal, Amit; Chandrasekaran, C V

    2015-01-01

    The aim of the study was to investigate the safety and anti-inflammatory effects of polysaccharide fraction (F1) of Curcuma longa extract (NR-INF-02) in classical rodent models of inflammation. F1 was evaluated for its acute oral toxicity and found to be safe upto 5000 mg/kg body weight in rats. The anti-inflammatory activity of F1 was evaluated in acute (carrageenan - induced paw edema; xylene - induced ear edema) and chronic (cotton pellet - induced granuloma) models of inflammation. The results of the study demonstrated that F1 significantly (p ≤ 0.05) inhibited carrageenan-induced paw edema at 1 h and 3 h at doses of 11.25, 22.5 and 45 mg/kg body weight in rats. Also, F1 at doses of 15.75, 31.5 and 63 mg/kg significantly inhibited the xylene induced ear edema in mice. In a chronic model, F1 at 11.25, 22.5 and 45 mg/kg doses produced significant reduction of wet and dry weights of cotton pellets in rats. Overall results indicated that F1 of NR-INF-02 significantly attenuated acute and chronic inflammation in rodent models. This study emphasizes on the importance of Curcuma longa polysaccharide's role in acute and chronic inflammation.

  2. Kinetic characteristics and modelling of growth and substrate removal by Alcaligenes faecalis strain NR.

    Science.gov (United States)

    Chen, Jie; Zhao, Bin; An, Qiang; Wang, Xia; Zhang, Yi Xin

    2016-04-01

    Alcaligenes faecalis strain NR has the capability of simultaneous ammonium and organic carbon removal under sole aerobic conditions. The growth and substrate removal characteristics of A. faecalis strain NR were studied and appropriate kinetic models were developed. The maximum substrate removal rate of NH4 (+)-N and TOC were determined as 2.27 mg NH4 (+)-N/L/h and 30.00 mg TOC/L/h, respectively with initial NH4 (+)-N = 80 mg/L and TOC = 800 mg/L. Single-substrate models and double-substrate models based on Monod, Contois, Moser and Teissier were employed to describe the bioprocess kinetic coefficients. As a result, two double-substrate models, Teissier-Contois and Contois-Contois, were considered to be appropriate to model growth kinetics with both NH4 (+)-N and TOC as limiting substrates. The kinetic constants of maximum growth rate (μ max) and half-saturation constant (K S and B S) were obtained by solving multiple equations with regression. This work can be used to further understand and predict the performance of heterotrophic nitrifiers, and thus provides specific guidance of these functional strains in practical wastewater treatment process.

  3. Nr4a1-Dependent Ly6Clow Monocytes Monitor Endothelial Cells and Orchestrate Their Disposal

    Science.gov (United States)

    Carlin, Leo M.; Stamatiades, Efstathios G.; Auffray, Cedric; Hanna, Richard N.; Glover, Leanne; Vizcay-Barrena, Gema; Hedrick, Catherine C.; Cook, H. Terence; Diebold, Sandra; Geissmann, Frederic

    2013-01-01

    Summary The functions of Nr4a1-dependent Ly6Clow monocytes remain enigmatic. We show that they are enriched within capillaries and scavenge microparticles from their lumenal side in a steady state. In the kidney cortex, perturbation of homeostasis by a TLR7-dependent nucleic acid “danger” signal, which may signify viral infection or local cell death, triggers Gαi-dependent intravascular retention of Ly6Clow monocytes by the endothelium. Then, monocytes recruit neutrophils in a TLR7-dependent manner to mediate focal necrosis of endothelial cells, whereas the monocytes remove cellular debris. Prevention of Ly6Clow monocyte development, crawling, or retention in Nr4a1−/−, Itgal−/−, and Tlr7host−/−BM+/+ and Cx3cr1−/− mice, respectively, abolished neutrophil recruitment and endothelial killing. Prevention of neutrophil recruitment in Tlr7host+/+BM−/− mice or by neutrophil depletion also abolished endothelial cell necrosis. Therefore, Ly6Clow monocytes are intravascular housekeepers that orchestrate the necrosis by neutrophils of endothelial cells that signal a local threat sensed via TLR7 followed by the in situ phagocytosis of cellular debris. PMID:23582326

  4. Characterization of vNr-13, the first alphaherpesvirus gene of the bcl-2 family

    International Nuclear Information System (INIS)

    Aouacheria, Abdel; Banyai, Michelle; Rigal, Dominique; Schmidt, Carl J.; Gillet, Germain

    2003-01-01

    The Bcl-2 family, including antiapoptotic and proapoptotic members, plays key regulating roles in programmed cell death. We report the characterization of a new member of the bcl-2 family, encoded by herpesvirus of turkeys (HVT). The product of this gene shares 80% homology with Nr-13, an apoptosis inhibitor, which is overexpressed in avian cells transformed by the v-src oncogene. This new gene, that we propose to call vnr-13, is the first member of the bcl-2 family to be isolated among α-herpesviruses. Results from cells expressing the HVT-vnr-13 gene product show that the encoded protein inhibits apoptosis and also reduces the rate of cellular proliferation. Contrary to all bcl-2 homologues found in γ-herpesvirus, which are intronless, vnr-13 has the same organization as the cellular nr-13 gene. Hence, the HVT vnr-13 gene may have been acquired from a reverse transcriptase product of an unspliced precursor RNA, or via direct recombination with the host chromosomal DNA

  5. Development of radiotracers for imaging NR2B subtype NMDA receptors with positron emission tomography; Developpement de radiotraceurs pour la visualisation des recepteurs NMDA de sous-type NR2B par tomographie par emission de positons

    Energy Technology Data Exchange (ETDEWEB)

    Labas, R

    2007-07-01

    The aim of this thesis was to develop new radioactive tracers for imaging NR2B subtype NMDA receptors with positron emission tomography. Several compounds including 4-(4-fluoro-benzyl)piperidine and presenting interesting in vivo biological properties were the object of a labelling with a positrons emitter atom ({sup 11}C or {sup 18}F)

  6. Blending of NR/BR.EPDM by reactive processing for tire sidewall applications. Part III - Assessment of the blend ozone- and fatique-resistance in comparison with a conventional NR/BR compound.

    NARCIS (Netherlands)

    Sahakaro, Kannika; Naskar, N.; Datta, Rabin; Baaij, Johan; Noordermeer, Jacobus W.M.

    2007-01-01

    The NR/BR blend compound formulations for tire sidewall applications contain a set of stabilizers added to prevent degradation mainly due to oxygen, ozone, and heat. 6PPD is the most effective and widely used antiozonant in tire compounds, but is a highly staining material causing a surface

  7. Nitrogen fixation in Asaia sp. (family Acetobacteraceae).

    Science.gov (United States)

    Samaddar, Neeloy; Paul, Arundhati; Chakravorty, Somnath; Chakraborty, Writachit; Mukherjee, Joydeep; Chowdhuri, Debarati; Gachhui, Ratan

    2011-08-01

    The genus Asaia (family Acetobacteraceae) was first introduced with a single species-Asaia bogorensis and later six more species were described namely A. siamensis, A. krungthepensis, A. lannaensis, A. platycodi, A. prunellae, and A. astilbes. Acetobacteraceae family has been divided into ten genera but, only three of them include nitrogen fixing species: Gluconacetobacter, Acetobacter, and Swaminathania. This article originated from our study primarily aimed to isolate new endosymbiotic nitrogen fixer among Acetobacteraceae during which we have isolated, for the first time in India, four different strains of Asaia sp. from three different sources: Michalia champaca flower, Anopheles mosquito, and ant Tetraponera rufonigra. All the endosymbiotic strains isolated possess the ability to fix nitrogen. Evidence for both nitrogenase activity and the presence of nifH gene in isolated Asaia sp. is presented. Asaia bogorensis (MTCC 4041(T)) and A. siamensis (MTCC 4042(T)), two of the validated type strains available from the repository, were tested positive for the presence of functional nitrogenase. The nifH gene sequences from these type strains were also confirmed and compared with other nitrogen fixing members of the family Acetobacteraceae. Our result corroborate with the previous reports that Asaia sp. are indeed widely distributed in nature but this is the first time demonstration of their functional nitrogenase activity. This study shows Asaia sp. as fourth genera of nitrogen fixing bacteria in the family Acetobacteraceae.

  8. Molecular characterization of Trichoderma sp. isolated from ...

    African Journals Online (AJOL)

    The genetic relatedness among 15 isolates of Trichoderma sp. was analyzed with six micro-satellite primers. ISSR profiles showed 83.7% genetic diversity among the isolates with the formation of four clusters. Analysis of dendrogram revealed that similarity coefficient ranged from 0.27 to 0.95. ITS-PCR of rDNA region with ...

  9. Som man spørger

    DEFF Research Database (Denmark)

    Bjerg, Lars

    Indhold: Input og outputinterview ; Tre interviewtyper ; Tre grundprincipper ; De syv dødssynder ; Åbne, simple og neutrale spørgsmål ; Skuffespørgsmål ; Fokus ; Aktiv lytning ; Mål ; Strategi ; Abstrakt og konkret ; Det kritiske interview ; Kilder med et budskab ; Oldfruens fif ; Resumé i punktform...

  10. POTENSI MELANOTUS SP. DALAM MENDEGRADASI LIGNIN

    Directory of Open Access Journals (Sweden)

    NUNIK SULISTINAH

    2008-06-01

    Full Text Available Ten isolates of fungus were isolated from oil palm stem at oil palm plantation in Medan All of them were tested its abilities to degrade lignin. The results showed that one of them was able to grow on ligninase media and the fungi has the ability to degrade ligin. The fungi is identified as Melanotus sp.

  11. Biosorption of uranium by Azolla, SP, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Ludmila C.; Alves, Eliakim G.; Marumo, Julio T., E-mail: lcvieira@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Ferreira, Rafael V. de P., E-mail: rafael@itatijuca.com [Itatijuca Biotech, Sao Paulo, SP (Brazil); Canevesi, Rafael L.S.; Silva, Edson A., E-mail: edson.silva2@unioeste.br [Universidade Estadual do Oeste Parana (UNIOESTE), Toledo, PR (Brazil)

    2015-07-01

    Radioactive liquid waste needs special attention and requires suitable treatment before deposition. Among the potential technologies under development for the treatment of liquid radioactive wastes the biosorption has been highlighted by being an efficient and low cost technique. Biosorption process involves the exchange of ions contained in the biomass matrix by others present in solution. There are many biomasses that could be applied in treatment of radioactive wastes, for example, agricultural residues and macrophyte. The aim of this study is evaluate the ability of the Azolla sp., a floating aquatic plant, to absorb uranium in solution. Azolla sp. is a macrophyte that has been used to treat effluents containing heavy metals. The biosorption capacity of uranium by Azolla sp. was experimentally determined and modeled by isotherms. Experiments were performed to determine metal uptake, and then the solutions were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES). The isotherms applied to model the data was Langmuir, Freundlich, Sips Toth, Redlich Peternson, Two-Site-Langmuir, Radke Prausnitz to develop a technique for the treatment of radioactive liquid waste generated at the Nuclear and Energy Research Institute (IPEN-CNEN/SP), Brazil. (author)

  12. Biosorption of uranium by Azolla, SP, Brazil

    International Nuclear Information System (INIS)

    Vieira, Ludmila C.; Alves, Eliakim G.; Marumo, Julio T.; Ferreira, Rafael V. de P.; Canevesi, Rafael L.S.; Silva, Edson A.

    2015-01-01

    Radioactive liquid waste needs special attention and requires suitable treatment before deposition. Among the potential technologies under development for the treatment of liquid radioactive wastes the biosorption has been highlighted by being an efficient and low cost technique. Biosorption process involves the exchange of ions contained in the biomass matrix by others present in solution. There are many biomasses that could be applied in treatment of radioactive wastes, for example, agricultural residues and macrophyte. The aim of this study is evaluate the ability of the Azolla sp., a floating aquatic plant, to absorb uranium in solution. Azolla sp. is a macrophyte that has been used to treat effluents containing heavy metals. The biosorption capacity of uranium by Azolla sp. was experimentally determined and modeled by isotherms. Experiments were performed to determine metal uptake, and then the solutions were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES). The isotherms applied to model the data was Langmuir, Freundlich, Sips Toth, Redlich Peternson, Two-Site-Langmuir, Radke Prausnitz to develop a technique for the treatment of radioactive liquid waste generated at the Nuclear and Energy Research Institute (IPEN-CNEN/SP), Brazil. (author)

  13. Carbon sp chains in graphene nanoholes

    DEFF Research Database (Denmark)

    Castelli, Ivano Eligio; Ferri, Nicola; Onida, Giovanni

    2012-01-01

    Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. We...

  14. Lipid contents of the sponge Haliclona sp.

    Digital Repository Service at National Institute of Oceanography (India)

    Parameswaran, P.S.; Das, B.; Kamat, S.Y.

    Several fatty acids, sterols, batyl alcohol and its analogs and an N-acylated sphingosine (ceramide) have been isolated from the lipid fraction of the extract of the sponge Haliclona sp. The major sterol is found to be cholesterol (54%), followed...

  15. Test af spørgeskema

    DEFF Research Database (Denmark)

    Hermansen, Jonathan

    2017-01-01

    Denne bog gennemgår alle surveyarbejdets faser og giver en praktisk indføring i •design af undersøgelsen og udvælgelse af stikprøver, •formulering af spørgeskemaer samt indsamling og kodning af data, •metoder til at analysere resultaterne....

  16. Botrallin from the endophytic fungus Hyalodendriella sp ...

    African Journals Online (AJOL)

    use

    2011-12-12

    Dec 12, 2011 ... Bioassay-guided fractionation of the crude methanol extract of the mycelia from the endophytic fungus. Hyalodendriella sp. Ponipodef12, associated with the hybrid 'Neva' of Populus deltoides Marsh × P. nigra L., led to the isolation of one compound coded as P12-1 which was identified as botrallin (1,7-.

  17. C (sp2)–C (sp2) cross coupling reaction catalyzed by a palladacycle ...

    Indian Academy of Sciences (India)

    (sp2) cross coupling reaction catalyzed by a palladacycle phosphine complex: A simple and sustainable protocol in aqueous media. Seyyed Javad Sabounchei Marjan Hosseinzadeh. Articles Volume 127 Issue 11 November 2015 pp 1919- ...

  18. Comprehensive Survey of Genetic Diversity in Chloroplast Genomes and 45S nrDNAs within Panax ginseng Species

    Science.gov (United States)

    Kim, Kyunghee; Lee, Sang-Choon; Lee, Junki; Lee, Hyun Oh; Joh, Ho Jun; Kim, Nam-Hoon; Park, Hyun-Seung; Yang, Tae-Jin

    2015-01-01

    We report complete sequences of chloroplast (cp) genome and 45S nuclear ribosomal DNA (45S nrDNA) for 11 Panax ginseng cultivars. We have obtained complete sequences of cp and 45S nrDNA, the representative barcoding target sequences for cytoplasm and nuclear genome, respectively, based on low coverage NGS sequence of each cultivar. The cp genomes sizes ranged from 156,241 to 156,425 bp and the major size variation was derived from differences in copy number of tandem repeats in the ycf1 gene and in the intergenic regions of rps16-trnUUG and rpl32-trnUAG. The complete 45S nrDNA unit sequences were 11,091 bp, representing a consensus single transcriptional unit with an intergenic spacer region. Comparative analysis of these sequences as well as those previously reported for three Chinese accessions identified very rare but unique polymorphism in the cp genome within P. ginseng cultivars. There were 12 intra-species polymorphisms (six SNPs and six InDels) among 14 cultivars. We also identified five SNPs from 45S nrDNA of 11 Korean ginseng cultivars. From the 17 unique informative polymorphic sites, we developed six reliable markers for analysis of ginseng diversity and cultivar authentication. PMID:26061692

  19. Methylation of the Glucocorticoid Receptor (NR3C1 in Placenta is Associated with Infant Cry Acoustics

    Directory of Open Access Journals (Sweden)

    Stephen J Sheinkopf

    2016-06-01

    Full Text Available Epigenetic mechanisms regulating expression of the glucocorticoid receptor gene (NR3C1 promoter may influence behavioral and biological aspects of stress response in human infants. Acoustic features of infant crying are an indicator of neurobehavioral and neurological status not yet investigated in relation to epigenetic mechanisms. We examined NR3C1 methylation in placental tissue from a series of 120 healthy newborn infants in relation to a detailed set of acoustic features extracted from newborn infant cries. We identified significant associations of NR3C1 methylation with energy variation in infants’ cries as well as with the presence of very high fundamental frequency in cry utterances. The presence of high fundamental frequency in cry (above 1 kHz has been linked to poor vocal tract control, poor regulation of stress response, and may be an indicator of poor neurobehavioral integrity. Thus, these results add to evidence linking epigenetic alteration of the NR3C1 gene in the placenta to neurodevelopmental features in infants.

  20. The NMDAR subunit NR3A interacts with microtubule-associated protein 1S in the brain

    DEFF Research Database (Denmark)

    Eriksson, Maria; Samuelsson, Helena; Samuelsson, Eva-Britt

    2007-01-01

    When screening a brain cDNA library, we found that the N-methyl-D-aspartate receptor subunit NR3A binds to microtubule-associated protein (MAP) 1S/chromosome 19 open reading frame 5 (C19ORF5). The interaction was confirmed in vitro and in vivo, and binding of MAP1S was localized to the membrane......-proximal part of the NR3A C-terminus. MAP1S belongs to the same family as MAP1A and MAP1B, and was found to be abundant in both postnatal and adult rat brain. In hippocampal neurons the distribution-pattern of MAP1S resembled that of beta-tubulin III, but a fraction of the protein colocalized with synaptic...... markers synapsin and postsynaptic density protein 95 (PSD95), in beta-tubulin III-negative filopodia-like protrusions. There was coexistance between MAP1S and NR3A immunoreactivity in neurite shafts and occasionally in filopodia-like processes. MAP1S potentially links NR3A to the cytoskeleton, and may...

  1. 40 CFR 80.572 - What labeling requirements apply to retailers and wholesale purchaser-consumers of NR and NRLM...

    Science.gov (United States)

    2010-07-01

    ... PROGRAMS (CONTINUED) REGULATION OF FUELS AND FUEL ADDITIVES Motor Vehicle Diesel Fuel; Nonroad, Locomotive, and Marine Diesel Fuel; and ECA Marine Fuel Labeling Requirements § 80.572 What labeling requirements... retailers and wholesale purchaser-consumers of NR and NRLM diesel fuel and heating oil beginning June 1...

  2. Medtner: Konzert für Klavier und Orchester Nr. 1 c-Moll op. 33 / Volkmar Fischer

    Index Scriptorium Estoniae

    Fischer, Volkmar

    1992-01-01

    Uuest heliplaadist "Medtner: Konzert für Klavier und Orchester Nr. 1 c-Moll op. 33, Sonaten-Ballade für Klavier Fis-Dur op. 27. London Philharmonic Orchestra / Neeme Järvi". Chandos/Koch CD9039 (WD:54'28")

  3. "NR3C1" Methylation as a Moderator of the Effects of Maternal Support and Stress on Insecure Attachment Development

    Science.gov (United States)

    Bosmans, Guy; Young, Jami F.; Hankin, Benjamin L.

    2018-01-01

    We examined the prediction that the interaction between Glucocorticoid Receptor Gene ("NR3C1") methylation, stress, and experienced maternal support predicts anxious and avoidant attachment development. This was tested in a general population sample of 487 children and adolescents (44% boys, M[subscript age] = 11.84, SD[subscript age] =…

  4. NR/PP Thermoplastic Vulcanizates: Selection of Optimal Peroxide Type and Concentration in Relation to Mixing Conditions

    NARCIS (Netherlands)

    Thitithammawong, A.; Thitithammawong, A.; Nakason, C.; Sahakaro, Kannika; Sahakaro, K.; Noordermeer, Jacobus W.M.

    2007-01-01

    Thermoplastic vulcanizates (TPVs) from natural rubber (NR) and polypropylene (PP) were studied, prepared by dynamic vulcanization during melt mixing, using various peroxides to crosslink the rubber phase. The objective was to find a proper balance between degree of crosslinking of the rubber and

  5. Carbon monoxide alleviates lipopolysaccharide-induced oxidative stress injury through suppressing the expression of Fis1 in NR8383 cells

    Energy Technology Data Exchange (ETDEWEB)

    Shi, Jia [Department of Anesthesiology, Tianjin Nankai Hospital, Tianjin Medical University, Tianjin 300100 (China); Yu, Jian-bo, E-mail: yujianbo11@126.com [Department of Anesthesiology, Tianjin Nankai Hospital, Tianjin Medical University, Tianjin 300100 (China); Liu, Wei; Wang, Dan; Zhang, Yuan; Gong, Li-rong; Dong, Shu-an [Department of Anesthesiology, Tianjin Nankai Hospital, Tianjin Medical University, Tianjin 300100 (China); Liu, Da-quan [Department of Pharmacology, Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin 300100 (China)

    2016-11-15

    Acute respiratory distress syndrome (ARDS) is one of the most devastating complications of sepsis lacking of effective therapy. Mitochondrial dynamics undergoing continuous fusion and fission play a crucial role in mitochondrial structure and function. Fis1, as a small protein located on the outer membrane of mitochondria, has been thought to be an important protein mediated mitochondrial fission. During ARDS, alveolar macrophages suffer from increased oxidative stress and apoptosis, and also accompanied by disrupted mitochondrial dynamics. In addition, as one of the products of heme degradation catalyzed by heme oxygenase, carbon monoxide (CO) possesses powerful protective properties in vivo or in vitro models, such as anti-inflammatory, antioxidant and anti-apoptosis function. However, there is little evidence that CO alleviates oxidative stress damage through altering mitochondrial fission in alveolar macrophages. In the present study, our results showed that CO increased cell vitality, improved mitochondrial SOD activity, reduced reactive oxygen species (ROS) production and inhibited cell apoptosis in NR8383 exposed to LPS. Meanwhile, CO decreased the expression of Fis1, increased mitochondrial membrane potential and sustained elongation of mitochondria in LPS-incubated NR8383. Overall, our study underscored a critical role of CO in suppressing the expression of Fis1 and alleviating LPS- induced oxidative stress damage in alveolar macrophages. - Highlights: • LPS exposure triggered cell injury in NR8383. • CO alleviated LPS-induced oxidative stress damage in alveolar macrophages. • CO inhibited Fis1 levels and improved mitochondrial function in LPS-induced NR8383.

  6. Epigenetic Regulation of Placental "NR3C1": Mechanism Underlying Prenatal Programming of Infant Neurobehavior by Maternal Smoking?

    Science.gov (United States)

    Stroud, Laura R.; Papandonatos, George D.; Salisbury, Amy L.; Phipps, Maureen G.; Huestis, Marilyn A.; Niaura, Raymond; Padbury, James F.; Marsit, Carmen J.; Lester, Barry M.

    2016-01-01

    Epigenetic regulation of the placental glucocorticoid receptor gene ("NR3C1") was investigated as a mechanism underlying links between maternal smoking during pregnancy (MSDP) and infant neurobehavior in 45 mother-infant pairs (49% MSDP-exposed; 52% minorities; ages 18-35). The Neonatal Intensive Care Unit (NICU) Network Neurobehavioral…

  7. a-Copaene and quercivorol lures: Chemical analysis and efficacy for detection of Euwallacea nr. fornicatus (Coleoptera: Curculionidae: Scolytinae)

    Science.gov (United States)

    Invasive ambrosia beetles in the Euwallacea nr. fornicatus complex vector a fungal pathogen responsible for Fusarium dieback, a disease that impacts avocado (Persea americana) and numerous native trees in the USA, Israel, and other countries. Currently, these pests are detected with quercivorol lure...

  8. Nanocrystalline sp{sup 2} and sp{sup 3} carbons: CVD synthesis and applications

    Energy Technology Data Exchange (ETDEWEB)

    Terranova, M. L. [Università degli Studi di Roma “Tor Vergata,” via Della Ricerca Scientifica, Dipartimento di Scienze e Tecnologie Chimiche—MinimaLab (Italy); Rossi, M. [Università degli Studi di Roma “Sapienza,” via A. Scarpa, Dipartimento di Scienze di Base e Applicate per l’Ingegneria and Centro di Ricerca per le Nanotecnologie Applicate all’Ingegneria (CNIS) (Italy); Tamburri, E., E-mail: emanuela.tamburri@uniroma2.it [Università degli Studi di Roma “Tor Vergata,” via Della Ricerca Scientifica, Dipartimento di Scienze e Tecnologie Chimiche—MinimaLab (Italy)

    2016-11-15

    The design and production of innovative materials based on nanocrystalline sp{sup 2}- and sp{sup 3}-coordinated carbons is presently a focus of the scientific community. We present a review of the nanostructures obtained in our labs using a series of synthetic routes, which make use of chemical vapor deposition (CVD) techniques for the selective production of non-planar graphitic nanostructures, nanocrystalline diamonds, and hybrid two-phase nanostructures.

  9. Analisis Fosfor pada Cacing Tanah (Megascolex sp. dan Fridericia sp.) Secara Spektrofotometri Sinar Tampak

    OpenAIRE

    Safira, Cut Shafa

    2015-01-01

    Earthworm is natural resource which can be used for medication due to its highly amount of minerals. One of these minerals is phosphorus. The aim of this research are to identify, determine and know the difference content of phosphorus in Megascolex sp. and Fridericia sp. Qualitative analysis shows positive results with addition of ammonium molybdate 4% and BaCl2 5%. Quantitative analysis was done using visible spectrophotometer with ascorbic acid method, measuring blu-colored molybdenum ...

  10. Analysis of C. elegans NR2E nuclear receptors defines three conserved clades and ligand-independent functions

    Directory of Open Access Journals (Sweden)

    Weber Katherine P

    2012-06-01

    Full Text Available Abstract Background The nuclear receptors (NRs are an important class of transcription factors that are conserved across animal phyla. Canonical NRs consist of a DNA-binding domain (DBD and ligand-binding domain (LBD. While most animals have 20–40 NRs, nematodes of the genus Caenorhabditis have experienced a spectacular proliferation and divergence of NR genes. The LBDs of evolutionarily-conserved Caenorhabditis NRs have diverged sharply from their Drosophila and vertebrate orthologs, while the DBDs have been strongly conserved. The NR2E family of NRs play critical roles in development, especially in the nervous system. In this study, we explore the phylogenetics and function of the NR2E family of Caenorhabditis elegans, using an in vivo assay to test LBD function. Results Phylogenetic analysis reveals that the NR2E family of NRs consists of three broadly-conserved clades of orthologous NRs. In C. elegans, these clades are defined by nhr-67, fax-1 and nhr-239. The vertebrate orthologs of nhr-67 and fax-1 are Tlx and PNR, respectively. While the nhr-239 clade includes orthologs in insects (Hr83, an echinoderm, and a hemichordate, the gene appears to have been lost from vertebrate lineages. The C. elegans and C. briggsae nhr-239 genes have an apparently-truncated and highly-diverged LBD region. An additional C. elegans NR2E gene, nhr-111, appears to be a recently-evolved paralog of fax-1; it is present in C. elegans, but not C. briggsae or other animals with completely-sequenced genomes. Analysis of the relatively unstudied nhr-111 and nhr-239 genes demonstrates that they are both expressed—nhr-111 very broadly and nhr-239 in a small subset of neurons. Analysis of the FAX-1 LBD in an in vivo assay revealed that it is not required for at least some developmental functions. Conclusions Our analysis supports three conserved clades of NR2E receptors, only two of which are represented in vertebrates, indicating three ancestral NR2E genes in the

  11. Differences in nutrient uptake capacity of the benthic filamentous algae Cladophora sp., Klebsormidium sp. and Pseudanabaena sp. under varying N/P conditions.

    Science.gov (United States)

    Liu, Junzhuo; Vyverman, Wim

    2015-03-01

    The N/P ratio of wastewater can vary greatly and directly affect algal growth and nutrient removal process. Three benthic filamentous algae species Cladophora sp., Klebsormidium sp. and Pseudanabaena sp. were isolated from a periphyton bioreactor and cultured under laboratory conditions on varying N/P ratios to determine their ability to remove nitrate and phosphorus. The N/P ratio significantly influenced the algal growth and phosphorus uptake process. Appropriate N/P ratios for nitrogen and phosphorus removal were 5-15, 7-10 and 7-20 for Cladophora sp., Klebsormidium sp. and Pseudanabaena sp., respectively. Within these respective ranges, Cladophora sp. had the highest biomass production, while Pseudanabaena sp. had the highest nitrogen and phosphorus contents. This study indicated that Cladophora sp. had a high capacity of removing phosphorus from wastewaters of low N/P ratio, and Pseudanabaena sp. was highly suitable for removing nitrogen from wastewaters with high N/P ratio. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Anomalous self potential (sp) log signatures observed in a water ...

    African Journals Online (AJOL)

    Geophysical logging was done after drilling had been completed in a water well at Okwudor, South Eastern Nigeria. Three electric logs were run viz: Self Potential (SP), Resistivity N16″ and N64″ logs. An anomaly was observed in the SP log. The SP results from this well show some deviation from the standard norm.

  13. Biosorption of chromium by mangrove-derived Aplanochytrium sp ...

    African Journals Online (AJOL)

    The microbial dried biomass of Thraustochytrids is used as bioadsorbent for the removal of the chromium in aqueous solution. In this investigation, three species of Thraustochydrids namely Aplanochytrium sp., Thraustochytrium sp. and Schizochytrium sp. were tested for the efficiency of chromium accumulation by culturing ...

  14. Babesia peircei sp. nov. from the jackass penguin

    African Journals Online (AJOL)

    1992-01-09

    Jan 9, 1992 ... An avian piroplasm, Babesia peircei sp. nov. is described from the jackass penguin Spheniscus demersus. Morphological differences between Babesia peircei sp. nov. and the other valid Babesia spp. are discussed together with the possible vectors. 'n Voal-piroplasma, Babesia peircei sp. nov. afkomstig ...

  15. Occurrence of Aspergillus sp., Fusarium sp., and aflatoxins in corn hybrids with different systems of storage

    Directory of Open Access Journals (Sweden)

    Adriana Sbardelotto Di Domenico

    2016-01-01

    Full Text Available The aim of this study was to assess alternatives for viable corn storage for small rural properties in two annual storage experiments. A 4×5 factorial design was used with four types of storage (conventional bags, hermetic bags, metal silos and corncobs and five periods of storage (0, 3, 6, 9 and 12 months. We used corn hybrids 2B688RR and 30K73Hx cultivated in winter 2012 and summer 2012/2013 in the city of Dois Vizinhos, Paraná, Brazil. The moisture contents, counts of Aspergillus sp. and Fusarium sp., and the occurrence of aflatoxins (B1, B2, G1 and G2 were assessed. The kernels stored in hermetic bags had lower moisture contents. Aspergillus sp. and Fusarium sp. were observed in 20.37 and 86.11% of winter storage samples, respectively, and in 83.3 and 91.6% of summer storage samples, respectively. The storage system and time of storage had no influence on the occurrence of Aspergillus sp. and aflatoxins in the winter crop samples. The corncobs from the summer crop samples had the lowest counts of Aspergillus sp. and did not have aflatoxins. We detected aflatoxins at concentrations of 2.8-14.5 and 3-197.5 µg kg-1 in the winter and summer crop samples, respectively.

  16. Two new species of suctorians, Acineta satyanandani sp. nov. and Paracineta karunakarani sp. nov. epizoic on ostracods

    Digital Repository Service at National Institute of Oceanography (India)

    Santhakumari, V.

    Two, species of protozoic suctorians, Acineta satyanandani sp. nov. and Paracineta karunakarani sp. nov., are described. These were found attached on the body of the marine ostracod, Cypridina dentata (Muller), collected from the shelf and slope...

  17. The family Carabodidae (Acari, Oribatida) VIII. The genus Machadocepheus (first part) Machadocepheusleoneae sp. n. and Machadocepheusrachii sp. n. from Gabon.

    Science.gov (United States)

    Fernandez, Nestor; Theron, Pieter; Rollard, Christine; Leiva, Sergio

    2014-01-01

    The genus Machadocepheus, being one of the more complex genera of the Carabodidae family, is briefly outlined to demonstrate this complexity. Descriptions of two new species from Gabon, Machadocepheusleoneae sp. n. and Machadocepheusrachii sp. n. are given.

  18. Modulation of expression of the nuclear receptor NR0B2 (small heterodimer partner 1 and its impact on proliferation of renal carcinoma cells

    Directory of Open Access Journals (Sweden)

    Prestin K

    2016-08-01

    Full Text Available Katharina Prestin,1,* Maria Olbert,2,* Janine Hussner,1 Tamara L Isenegger,1 Daniel G Gliesche,1 Kerstin Böttcher,2 Uwe Zimmermann,3 Henriette E Meyer zu Schwabedissen1 1Department of Pharmaceutical Sciences, Biopharmacy, University of Basel, Basel, Switzerland; 2Center of Drug Absorption and Transport, Institute of Pharmacology, 3Department of Urology, University Medicine Greifswald, Greifswald, Germany *These authors contributed equally to this work Abstract: Mammalian nuclear receptors (NRs are transcription factors regulating the expression of target genes that play an important role in drug metabolism, transport, and cellular signaling pathways. The orphan and structurally unique receptor small heterodimer partner 1 (syn NR0B2 is not only known for its modulation of drug response, but has also been reported to be involved in hepatocellular carcinogenesis. Indeed, previous studies show that NR0B2 is downregulated in human hepatocellular carcinoma, suggesting that NR0B2 acts as a tumor suppressor via inhibition of cellular growth and activation of apoptosis in this tumor entity. The aim of our study was to elucidate whether NR0B2 may also play a role in other tumor entities. Comparing NR0B2 expression in renal cell carcinoma and adjacent nonmalignant transformed tissue revealed significant downregulation in vivo. Additionally, the impact of heterologous expression of NR0B2 on cell cycle progression and proliferation in cells of renal origin was characterized. Monitoring fluorescence intensity of resazurin turnover in RCC-EW cells revealed no significant differences in metabolic activity in the presence of NR0B2. However, there was a significant decrease of cellular proliferation in cells overexpressing this NR, and NR0B2 was more efficient than currently used antiproliferative agents. Furthermore, flow cytometry analysis showed that heterologous overexpression of NR0B2 significantly reduced the amount of cells passing the G1 phase, while on

  19. PRODUCTION AND PURIFICATION OF IgY ANTIBODIES AS A NOVEL TOOL TO PURIFY THE NR1 SUBUNIT OF NMDA RECEPTO

    Directory of Open Access Journals (Sweden)

    Edgar Antonio Reyes Montaño

    2011-12-01

    Full Text Available Producing polyclonal antibodies (IgY inchickens has advantages over those obtainedin other animal models, since theyhave been used as a tool for studyingdifferent proteins (NMDA glutamate receptorin our case, specifically the NR1subunit. We produced specific antibodiesagainst expression products by thealternative splicing of the gene encodingNMDA receptor NR1 subunit in adult ratbrain. Three peptides corresponding tothe splicing sites (N1, C1 and C2’ cassetteswere designed, synthesised and usedindividually as antigens in hens. Specificimmunoglobulins were purified fromyolks. The antibodies were then used forpurifying the NMDA receptor NR1 subunitusing affinity chromatography couplingthe three antibodies to the support.R

  20. The HR97 (NR1L) Group of Nuclear Receptors: A New Group up of Nuclear Receptors Discovered in Daphnia species

    OpenAIRE

    Li, Yangchun; Ginjupalli, Gautam K.; Baldwin, William S.

    2014-01-01

    The recently sequenced Daphnia pulex genome revealed the NR1L nuclear receptor group consisting of three novel receptors. Phylogenetic studies show that this group is related to the NR1I group (CAR/PXR/VDR) and the NR1J group (HR96), and were subsequently named HR97a/b/g. Each of the HR97 paralogs from Daphnia magna, a commonly used crustacean in toxicity testing, was cloned, sequenced, and partially characterized. Phylogenetic analysis indicates that the HR97 receptors are present in primiti...

  1. Mas-Related Gene (Mrg C Activation Attenuates Bone Cancer Pain via Modulating Gi and NR2B.

    Directory of Open Access Journals (Sweden)

    Yu'e Sun

    Full Text Available This study is to investigate the role of Mas-related gene (Mrg C in the pathogenesis and treatment of bone cancer pain (BCP.BCP mouse model was established by osteosarcoma cell inoculation. Pain-related behaviors were assessed with the spontaneous lifting behavior test and mechanical allodynia test. Expression levels of MrgC, Gi, and NR2B in the spinal cord were detected with Western blot analysis and immunohistochemistry.Pain-related behavior tests showed significantly increased spontaneous flinches (NSF and decreased paw withdrawal mechanical threshold (PWMT in mouse models of BCP. Western blot analysis showed that, compared with the control group and before modeling, all the expression levels of MrgC, Gi, and NR2B in the spinal cord of BCP mice were dramatically elevated, which were especially increased at day 7 after operation and thereafter, in a time-dependent manner. Moreover, the treatment of MrgC agonist BAM8-22 significantly up-regulated Gi and down-regulated NR2B expression levels, in the spinal cord of BCP mice, in a time-dependent manner. On the other hand, anti-MrgC significantly down-regulated Gi expression, while dramatically up-regulated NR2B expression, in the BCP mice. Similar results were obtained from the immunohistochemical detection. Importantly, BAM8-22 significantly attenuated the nociceptive behaviors in the BCP mice.Our results indicated the MrgC-mediated Gi and NR2B expression alterations in the BCP mice, which might contribute to the pain hypersensitivity. These findings may provide a novel strategy for the treatment of BCP in clinic.

  2. First report of Giardia sp. and Cryptosporidium sp. in fecal samples in blue macaw (Anodorhynchus hyacinthinus) in southern Brazil

    OpenAIRE

    Matheus Hillard Farret; Vinicius da Rosa Fanfa; Luisa Ragagnin; Aleksandro Schafer da Silva; Silvia Gonzalez Monteiro

    2010-01-01

    This study reports the gastrointestinal parasitism by Giardia sp. and Cryptosporidium sp. in blue macaw (Anodorhynchus hyacinthinus) in the southern region of Brazil. Fecal samples of two species kept in captivity in the state of Rio Grande do Sul were analyzed by the direct smear method, centrifugal flotation technique with zinc sulfate and Kinyoun staining technique for research of parasites. Mixed infection by eggs of Capillaria, cysts of Giardia sp. and oocysts of Cryptosporidium sp. was ...

  3. Agromyces italicus sp. nov., Agromyces humatus sp. nov. and Agromyces lapidis sp. nov., isolated from Roman catacombs.

    Science.gov (United States)

    Jurado, Valme; Groth, Ingrid; Gonzalez, Juan M; Laiz, Leonila; Schuetze, Barbara; Saiz-Jimenez, Cesareo

    2005-03-01

    A polyphasic study was carried out to clarify the taxonomic positions of three Gram-positive isolates from the Catacombs of Domitilla, Rome (Italy). 16S rRNA gene sequence comparisons placed these strains within the genus Agromyces. The morphological and chemotaxonomic characteristics of these isolates were consistent with the description of the genus Agromyces. The three isolates could be readily distinguished from one another and from representatives of all Agromyces species with validly published names by a broad range of phenotypic characteristics and DNA-DNA relatedness studies. Therefore, these isolates are proposed to represent three novel species of the genus Agromyces, Agromyces italicus sp. nov. (type strain CD1(T)=HKI 0325(T)=DSM 16388(T)=NCIMB 14011(T)), Agromyces humatus sp. nov. (type strain CD5(T)=HKI 0327(T)=DSM 16389(T)=NCIMB 14012(T)) and Agromyces lapidis sp. nov. (type strain CD55(T)=HKI 0324(T)=DSM 16390(T)=NCIMB 14013(T)).

  4. Cryptotrichosporon argae sp. nov., Cryptotrichosporon brontae sp. nov. and Cryptotrichosporon steropae sp. nov., isolated from forest soils.

    Science.gov (United States)

    Pontes, Ana; Röhl, Oliver; Maldonado, Cristina; Yurkov, Andrey M; Sampaio, José Paulo

    2017-09-01

    Yeast strains belonging to the basidiomycetous genus Cryptotrichosporon were isolated from forest soils in Serra da Arrábida Natural Park in Portugal. Similar to the already-known representatives of this genus, the new isolates formed pigmented colonies of a distinctive pale orange colour. Phylogenetic analyses employing concatenated sequences of the D1/D2 domains of the 26S (large subunit) rRNA gene and the internal transcribed spacer (ITS) region supported the recognition of three novel species: Cryptotrichosporon argae sp. nov. (type strain CM 19T=CBS 14376T=PYCC 7010T=DSM 104550T; MycoBank accession number MB 817168), Cryptotrichosporon brontae sp. nov. (type strain CM 1562T=CBS 14303T=PYCC 7011T=DSM 104551T; MycoBank accession number MB 817077) and Cryptotrichosporon steropae sp. nov. (type strain OR 395T=CBS 14302T=PYCC 7012T=DSM 104552T; MycoBank accession number MB 817078).

  5. Fitoremediasi limbah budidaya sidat menggunakan filamentous algae (Spirogyra sp.

    Directory of Open Access Journals (Sweden)

    Tri Apriadi

    2014-09-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui potensi dari filamentous algae (Spirogyra sp. sebagai agen bioremediasi dalam mereduksi kandungan bahan organik limbah budidaya sidat. Penelitian menggunakan rancangan acak lengkap dengan perlakuan perbedaan dosis limbah (25 %, 50 %, 75 %, 100%. Wadah penelitian berupa akuarium resirkulasi menggunakan sistem carrousel. Dilakukan pengukuran secara rutin terhadap beberapa parameter kualitas air serta perubahan bobot Spirogyra sp. selama dua minggu retensi. Diperoleh hasil bahwa penurunan konsentrasi bahan organik menggunakan Spirogyra sp. berlangsung efektif hingga hari keenam. Spirogyra sp. mampu mentolelir limbah budidaya sidat pada dosis limbah 25% dan 50%. Spirogyra sp. pada perlakuan dosis limbah 50% memiliki kemampuan yang lebih baik dalam menurunkan bahan organik limbah budidaya sidat.

  6. A superhard sp3 microporous carbon with direct bandgap

    Science.gov (United States)

    Pan, Yilong; Xie, Chenlong; Xiong, Mei; Ma, Mengdong; Liu, Lingyu; Li, Zihe; Zhang, Shuangshuang; Gao, Guoying; Zhao, Zhisheng; Tian, Yongjun; Xu, Bo; He, Julong

    2017-12-01

    Carbon allotropes with distinct sp, sp2, and sp3 hybridization possess various different properties. Here, a novel all-sp3 hybridized tetragonal carbon, namely the P carbon, was predicted by the evolutionary particle swarm structural search. It demonstrated a low density among all-sp3 carbons, due to the corresponding distinctive microporous structure. P carbon is thermodynamically stable than the known C60 and could be formed through the single-walled carbon nanotubes (SWCNTs) compression. P carbon is a direct bandgap semiconductor displaying a strong and superhard nature. The unique combination of electrical and mechanical properties constitutes P carbon a potential superhard material for semiconductor industrial fields.

  7. Sp6 and Sp8 Transcription Factors Control AER Formation and Dorsal-Ventral Patterning in Limb Development

    Science.gov (United States)

    Haro, Endika; Delgado, Irene; Junco, Marisa; Yamada, Yoshihiko; Mansouri, Ahmed; Oberg, Kerby C.; Ros, Marian A.

    2014-01-01

    The formation and maintenance of the apical ectodermal ridge (AER) is critical for the outgrowth and patterning of the vertebrate limb. The induction of the AER is a complex process that relies on integrated interactions among the Fgf, Wnt, and Bmp signaling pathways that operate within the ectoderm and between the ectoderm and the mesoderm of the early limb bud. The transcription factors Sp6 and Sp8 are expressed in the limb ectoderm and AER during limb development. Sp6 mutant mice display a mild syndactyly phenotype while Sp8 mutants exhibit severe limb truncations. Both mutants show defects in AER maturation and in dorsal-ventral patterning. To gain further insights into the role Sp6 and Sp8 play in limb development, we have produced mice lacking both Sp6 and Sp8 activity in the limb ectoderm. Remarkably, the elimination or significant reduction in Sp6;Sp8 gene dosage leads to tetra-amelia; initial budding occurs, but neither Fgf8 nor En1 are activated. Mutants bearing a single functional allele of Sp8 (Sp6−/−;Sp8+/−) exhibit a split-hand/foot malformation phenotype with double dorsal digit tips probably due to an irregular and immature AER that is not maintained in the center of the bud and on the abnormal expansion of Wnt7a expression to the ventral ectoderm. Our data are compatible with Sp6 and Sp8 working together and in a dose-dependent manner as indispensable mediators of Wnt/βcatenin and Bmp signaling in the limb ectoderm. We suggest that the function of these factors links proximal-distal and dorsal-ventral patterning. PMID:25166858

  8. Bioakumulasi logam berat Cu oleh Bacillus sp

    Directory of Open Access Journals (Sweden)

    Riesta Primaharinastiti

    2012-02-01

    Full Text Available The research was conducted to investigate the ability of Bacillus sp in accumulating Cu and how much it can be acumulated. Themedium used to growth the bacterium was Nutrient Broth and Atomic Absorption Spectrophotometry methods was used to assay theCu, both in the cells and medium. The result of this study showed that Bacillus sp incubated in the Nutrient Broth medium containing10 ppm of Cu, with continuous stirring in the room temperature was able to reduce Cu in the medium 8.912–12.623% and accumulateCu in the cell 0.1149–0.1400 %/mg cells. Based on this result, it is necessary to develop more studies to find out what factors thatinfluence the accumulation process and to optimize the bioprocess.

  9. Analysis list: SP2 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SP2 Blood,Liver,Pluripotent stem cell + hg19 http://dbarchive.biosciencedbc.jp/kyus...hu-u/hg19/target/SP2.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/SP2.5.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/hg19/target/SP2.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.B...lood.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.Liver.tsv,http://dbarchive.bioscience...dbc.jp/kyushu-u/hg19/colo/SP2.Pluripotent_stem_cell.tsv http://dbarchive.biosciencedbc

  10. PNNL Apatite Investigation at 100-NR-2 Quality Assurance Project Plan

    Energy Technology Data Exchange (ETDEWEB)

    Fix, N. J.

    2009-04-02

    In 2004, the U.S. Department of Energy, Fluor Hanford, Inc., Pacific Northwest National Laboratory (PNNL), and the Washington Department of Ecology agreed that the long-term strategy for groundwater remediation at the 100-N Area would include apatite sequestration as the primary treatment, followed by a secondary treatment if necessary. Since then, the agencies have worked together to agree on which apatite sequestration technology has the greatest chance of reducing strontium-90 flux to the Columbia River. This Quality Assurance Project Plan provides the quality assurance requirements and processes that will be followed by staff working on the PNNL Apatite Investigation at 100-NR-2 Project. The plan is designed to be used exclusively by project staff.

  11. Masalah dan Cara Mengatasi Sistem Informasi Akuntansi Siklus Pengeluaran Kas di PT NR

    Directory of Open Access Journals (Sweden)

    Engelwati Gani

    2014-11-01

    Full Text Available A problem in accounting information systems of expenditure cash cycle in PT NR is estimated purchase raw materials often left over and valuable. To overcome it, accounting information with computer-based systems was designed related to raw material purchase that is expected to speed up procurement process and avoid fraud and error. The system processes waste materials that will be stored and used properly, so as to increase efficiency in purchasing raw materials. The analysis used for the construction of this system was business process analysis, problem identification, and analysis of information needs. The result achieved is accounting information systems of expenditure cash cycle to overcome the problem existing in the company. Research concludes the use of information systems can speed up purchase process and avoid fraud. Raw material management with system will make it easier to perform the calculation of actual costs and improve internal control.

  12. Evaluation of EPDM Addition in NR/BR Compound by Reactive Mixing

    Directory of Open Access Journals (Sweden)

    M. Ehsani

    2013-01-01

    Full Text Available Improvements in the properties of ordinary sidewall compound (NR/BR with the addition of EPDM by reactive processing method were evaluated. The results show that ozone resistance of these sidewalls was improved with increased addition of EPDM. Also the tensile strength and elongation-at-break were increased in comparison with NRBREPDM compounds that were made by non-reactive processing. SEM Photographs show that the reactive processing method helps to improve the compatibility of these three elastomers compared with NRBREPDM system made by non-reactive processing. The results of fatigue-to-failure and tear resistance show that compounds prepared by reactive processing have a better property in comparison with non-reactive samples. Tensile and elongation-at-break of the reactive samples were superior to the non-reactive samples after aging.

  13. OLPT CONDUCTIVITY IN WOLLASTONITE INLAID NR/SBR TYPE ELASTOMER BASED MATERIAL

    Directory of Open Access Journals (Sweden)

    E. Şentürk

    2012-07-01

    Full Text Available The electrical properties of wollastonite inlaid NR/SBR type elastomer based material have been evaluated. Electrical properties of the samples were measured in the temperature range of 303 to 453 K and the frequency range of 100 Hz – 40 MHz. All electrically measured parameters were given anomalies at 385 K. Only one type of dielectric relaxation process have been observed for all measurements. Physical parameters characterizing the dielectric behavior have been obtained by fitting the experimental results in the modified Debye equation. The activation energy which is thermally activated by dielectric relaxation process have been calculated to be 0.58 eV. DC conductivity increasing by temperature has been explained with the help of VFT model whereas the AC one has been clarified by the OLPT model.

  14. Partnerstwo publiczno – prywatne w świetle Krajowego Standardu Rachunkowości nr 10

    Directory of Open Access Journals (Sweden)

    Dorota Adamek-Hyska

    2017-09-01

    Full Text Available Partnerstwo publiczno – prywatne w świetle Krajowego Standardu Rachunkowości nr 10 In Poland, the concept of public-private partnership generates many dilemmas in valuation, accounting records and the reporting of essential assets and liabilities. This issue is addressed by National Accounting Standard No. 10, which outlines the principles of PPP accounting in public and private accounting systems. The purpose of this paper is to analyze and evaluate the solutions adopted in National Accounting Standard No. 10 against the background of synthetic PPP characteristics. The research methods used are based on a critical analysis of national legislation, a comparative analysis of national and international accounting standards, and a literature analysis of public-private partnerships, public finances and financial accounting. The paper uses the conclusions from the observations of financial aspects of selected public-private partnership contracts as well as concession contracts for construction works and services in Poland

  15. Muscle Contraction Induces Acute Hydroxymethylation of the Exercise-Responsive Gene Nr4a3

    DEFF Research Database (Denmark)

    Pattamaprapanont, Pattarawan; Garde, Christian; Fabre, Odile

    2016-01-01

    promoters. Exercise induces dynamic DNA demethylation at gene promoters; however, the contribution of the demethylation precursor hydroxymethylcytosine is unknown. Given the evanescent nature of hydroxymethylcytosine, a muscle contraction model that allows for the collection of samples that are repeatedly...... stimulated over time is required to determine whether contraction-induced demethylation is preceded by changes in the hydroxymethylcytosine level. Here, we established an acute skeletal muscle contraction model to mimic the effects of acute exercise on gene expression. We used this model to investigate...... the effect of muscle contraction on DNA demethylation and hydroxymethylation. First, we performed an acute exercise study in healthy humans to identify an exercise-responsive gene that we could study in culture. We identified the nuclear receptor subfamily 4 group A member 3 (Nr4a3) gene with the highest...

  16. Superfield quantization in Sp(2) covariant formalism

    CERN Document Server

    Lavrov, P M

    2001-01-01

    The rules of the superfield Sp(2) covariant quantization of the arbitrary gauge theories for the case of the introduction of the gauging with the derivative equations for the gauge functional are generalized. The possibilities of realization of the expanded anti-brackets are considered and it is shown, that only one of the realizations is compatible with the transformations of the expanded BRST-symmetry in the form of super translations along the Grassmann superspace coordinates

  17. Carbon sp chains in graphene nanoholes

    Science.gov (United States)

    Castelli, Ivano E.; Ferri, Nicola; Onida, Giovanni; Manini, Nicola

    2012-03-01

    Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. We adopt a fair compromise of microscopic realism with a certain level of idealization in the model configurations, and predict a number of properties susceptible to comparison with experiment.

  18. Commercial technologies from the SP-100 program

    International Nuclear Information System (INIS)

    Truscello, V.C.; Fujita, T.; Mondt, J.F.

    1995-01-01

    For more than a decade, Jet Propulsion Labortory and Los Alamos have managed a multi-agency funded effort to develop a space reactor power system. This SP-100 Program has developed technologies required for space power systems that can be implemented in the industrial and commercial sectors to improve competitiveness in the global economy. Initial steps taken to transfer this technology from the laboratories to industrial and commercial entities within United States include: (1) identifying specific technologies having commercial potential; (2) distributing information describing the identified technologies and interacting with interested commercial and industrial entities to develop application-specific details and requirements; and (3) providing a technological data base that leads to transfer of technology or the forming of teaming arrangements to accomplish the transfer by tailoring the technology to meet application-specific requirements. SP-100 technologies having commercial potential encompass fabrication processes, devices, and components. Examples: a process for bonding refractory metals to graphite, a device to sense the position of an actuator and a component to enable rotating machines to operate without supplying lubrication (self-lubricating ball bearing). Shortly after the NASA Regional Technology Transfer Centers widely disseminated information covering SP-100 technologies, over one hundred expressions of interest were received, which indicate that there is a large potential benefit in transferring SP-100 technology. Interactions with industrial and commercial entities have identified a substantial need for creating teaming arrangements involving the interested entity and personnel from laboratories and their contractors, who have the knowledge and ability to tailor the technology to meet application-specific requirements. copyright 1995 American Institute of Physics

  19. Aquatic studies at the 100-HR-3 and 100-NR-1 operable units

    International Nuclear Information System (INIS)

    Cushing, C.E.

    1993-04-01

    Pacific Northwest Laboratory initiated a program to characterize selected aquatic biological populations to determine (1) existing levels of inorganic chemical and radionuclide contamination, and (2) the populations' suitability as indicators of chemical releases during cleanup activities at the US Department of Energy's Hanford Site. Following work plans for the ground-water operable units, lower trophic levels in the aquatic habitat (periphyton and caddisfly larvae) were evaluated for contaminants at the 100-HR-3 Operable Unit and 100-NR-1 Operable Unit. The results were evaluated to determine the need for further sampling. If the results showed no significant contamination compared to upriver levels, sampling would be discontinued. The periphyton community appears to be suitable for determining contamination levels. Baseline concentrations for stable chromium were established and will be useful for comparing samples collected when contaminant release is expected. Concentrations of 60 Co, 90 Sr, and 137 Cs in periphyton were essentially below detectable limits, which will also make this community useful in detecting potential releases of radionuclides during cleanup activities. Levels for both stable chromium and radionuclides were essentially below detection limits for caddisfly larvae. Thus, these organisms may be used to monitor suspected contaminant releases from cleanup activities; if concentrations exceed detection limits, they may be related to these activities. Two candidate threatened and endangered species of molluscs occur in the Hanford Reach of the Columbia River. These are the shortface lanx (Fisherola nuttalli), which is a Washington State candidate species, and the Columbia pebblesnail (Fluminicola columbiana), which is both a state and federal candidate species. Specimens of the shortface lanx were observed in the vicinity of N Springs (100-NR-1 Operable Unit); they likely occur throughout this area

  20. HPA axis dysregulation, NR3C1 polymorphisms and glucocorticoid receptor isoforms imbalance in metabolic syndrome.

    Science.gov (United States)

    Martins, Clarissa Silva; Elias, Daniel; Colli, Leandro Machado; Couri, Carlos Eduardo; Souza, Manoel Carlos L A; Moreira, Ayrton C; Foss, Milton C; Elias, Lucila L K; de Castro, Margaret

    2017-03-01

    Metabolic syndrome (MetS) shares several similarities with hypercortisolism. To evaluate hypothalamic-pituitary-adrenal (HPA) axis sensitivity to dexamethasone (DEX), NR3C1 single nucleotide polymorphisms (SNPs), and expression of glucocorticoid receptor (GR) isoforms and cytokines in peripheral immune cells of MetS patients and controls. Prospective study with 40 MetS patients and 40 controls was conducted at the Ribeirão Preto Medical School University Hospital. Plasma and salivary cortisol were measured in basal conditions and after 0.25, 0.5, and 1 mg of DEX given at 2300 h. In addition, p.N363S (rs6195), p.ER22/23EK (rs6189-6190), and BclI (rs41423247) SNPs were evaluated by quantitative polymerase chain reaction allelic discrimination. Exons 3 to 9 and exon/intron boundaries of NR3C1 were sequenced. GR isoforms and cytokines (IL1B, IL2, IL4, IL6, IL8, IL10, IFNγ, TNFα) expression were assessed by quantitative polymerase chain reaction. Plasma and salivary cortisol (nmol/L) after 1-mg DEX were higher in MetS patients compared with controls (PF: 70.2 ± 17.3 vs 37.9 ± 2.6, P = .02, and SF: 4.9 ± 1.7 vs 2.2 ± 0.3, P molecular mechanism of glucocorticoid resistance in MetS. Thus, HPA axis dysregulation might contribute to MetS pathogenesis. Copyright © 2016 John Wiley & Sons, Ltd.

  1. The human orphan nuclear receptor tailless (TLX, NR2E1 is druggable.

    Directory of Open Access Journals (Sweden)

    Cindy Benod

    Full Text Available Nuclear receptors (NRs are an important group of ligand-dependent transcriptional factors. Presently, no natural or synthetic ligand has been identified for a large group of orphan NRs. Small molecules to target these orphan NRs will provide unique resources for uncovering regulatory systems that impact human health and to modulate these pathways with drugs. The orphan NR tailless (TLX, NR2E1, a transcriptional repressor, is a major player in neurogenesis and Neural Stem Cell (NSC derived brain tumors. No chemical probes that modulate TLX activity are available, and it is not clear whether TLX is druggable. To assess TLX ligand binding capacity, we created homology models of the TLX ligand binding domain (LBD. Results suggest that TLX belongs to an emerging class of NRs that lack LBD helices α1 and α2 and that it has potential to form a large open ligand binding pocket (LBP. Using a medium throughput screening strategy, we investigated direct binding of 20,000 compounds to purified human TLX protein and verified interactions with a secondary (orthogonal assay. We then assessed effects of verified binders on TLX activity using luciferase assays. As a result, we report identification of three compounds (ccrp1, ccrp2 and ccrp3 that bind to recombinant TLX protein with affinities in the high nanomolar to low micromolar range and enhance TLX transcriptional repressive activity. We conclude that TLX is druggable and propose that our lead compounds could serve as scaffolds to derive more potent ligands. While our ligands potentiate TLX repressive activity, the question of whether it is possible to develop ligands to de-repress TLX activity remains open.

  2. Aquatic studies at the 100-HR-3 and 100-NR-1 operable units

    Energy Technology Data Exchange (ETDEWEB)

    Cushing, C.E.

    1993-04-01

    Pacific Northwest Laboratory initiated a program to characterize selected aquatic biological populations to determine (1) existing levels of inorganic chemical and radionuclide contamination, and (2) the populations' suitability as indicators of chemical releases during cleanup activities at the US Department of Energy's Hanford Site. Following work plans for the ground-water operable units, lower trophic levels in the aquatic habitat (periphyton and caddisfly larvae) were evaluated for contaminants at the 100-HR-3 Operable Unit and 100-NR-1 Operable Unit. The results were evaluated to determine the need for further sampling. If the results showed no significant contamination compared to upriver levels, sampling would be discontinued. The periphyton community appears to be suitable for determining contamination levels. Baseline concentrations for stable chromium were established and will be useful for comparing samples collected when contaminant release is expected. Concentrations of [sup 60]Co, [sup 90]Sr, and [sup 137]Cs in periphyton were essentially below detectable limits, which will also make this community useful in detecting potential releases of radionuclides during cleanup activities. Levels for both stable chromium and radionuclides were essentially below detection limits for caddisfly larvae. Thus, these organisms may be used to monitor suspected contaminant releases from cleanup activities; if concentrations exceed detection limits, they may be related to these activities. Two candidate threatened and endangered species of molluscs occur in the Hanford Reach of the Columbia River. These are the shortface lanx (Fisherola nuttalli), which is a Washington State candidate species, and the Columbia pebblesnail (Fluminicola columbiana), which is both a state and federal candidate species. Specimens of the shortface lanx were observed in the vicinity of N Springs (100-NR-1 Operable Unit); they likely occur throughout this area.

  3. Aquatic studies at the 100-HR-3 and 100-NR-1 operable units

    Energy Technology Data Exchange (ETDEWEB)

    Cushing, C.E.

    1993-04-01

    Pacific Northwest Laboratory initiated a program to characterize selected aquatic biological populations to determine (1) existing levels of inorganic chemical and radionuclide contamination, and (2) the populations` suitability as indicators of chemical releases during cleanup activities at the US Department of Energy`s Hanford Site. Following work plans for the ground-water operable units, lower trophic levels in the aquatic habitat (periphyton and caddisfly larvae) were evaluated for contaminants at the 100-HR-3 Operable Unit and 100-NR-1 Operable Unit. The results were evaluated to determine the need for further sampling. If the results showed no significant contamination compared to upriver levels, sampling would be discontinued. The periphyton community appears to be suitable for determining contamination levels. Baseline concentrations for stable chromium were established and will be useful for comparing samples collected when contaminant release is expected. Concentrations of {sup 60}Co, {sup 90}Sr, and {sup 137}Cs in periphyton were essentially below detectable limits, which will also make this community useful in detecting potential releases of radionuclides during cleanup activities. Levels for both stable chromium and radionuclides were essentially below detection limits for caddisfly larvae. Thus, these organisms may be used to monitor suspected contaminant releases from cleanup activities; if concentrations exceed detection limits, they may be related to these activities. Two candidate threatened and endangered species of molluscs occur in the Hanford Reach of the Columbia River. These are the shortface lanx (Fisherola nuttalli), which is a Washington State candidate species, and the Columbia pebblesnail (Fluminicola columbiana), which is both a state and federal candidate species. Specimens of the shortface lanx were observed in the vicinity of N Springs (100-NR-1 Operable Unit); they likely occur throughout this area.

  4. Sterols from the Madagascar sponge Fascaplysinopsis sp.

    Science.gov (United States)

    Aknin, Maurice; Gros, Emmanuelle; Vacelet, Jean; Kashman, Yoel; Gauvin-Bialecki, Anne

    2010-12-17

    The sponge Fascaplysinopsis sp. (order Dictyoceratida, Family Thorectidae) from the west coast of Madagascar (Indian Ocean) is a particularly rich source of bioactive nitrogenous macrolides. The previous studies on this organism led to the suggestion that the latter should originate from associated microsymbionts. In order to evaluate the influence of microsymbionts on lipid content, 10 samples of Fascaplysinopsis sp. were investigated for their sterol composition. Contrary to the secondary metabolites, the sterol patterns established were qualitatively and quantitatively stable: 14 sterols with different unsaturated nuclei, Δ(5), Δ(7) and Δ(5,7), were identified; the last ones being the main sterols of the investigated sponges. The chemotaxonomic significance of these results for the order Dictyoceratida is also discussed in the context of the literature. The conjugated diene system in Δ(5,7) sterols is known to be unstable and easily photo-oxidized during storage and/or experiments to produce 5α,8α-epidioxy sterols. However, in this study, no 5α,8α-epidioxysterols (or only trace amounts) were observed. Thus, it was supposed that photo-oxidation was avoided thanks to the natural antioxidants detected in Fascaplysinopsis sp. by both the DPPH and β-caroten bleaching assays.

  5. Sterols from the Madagascar Sponge Fascaplysinopsis sp.

    Directory of Open Access Journals (Sweden)

    Yoel Kashman

    2010-12-01

    Full Text Available The sponge Fascaplysinopsis sp. (order Dictyoceratida, Family Thorectidae from the west coast of Madagascar (Indian Ocean is a particularly rich source of bioactive nitrogenous macrolides. The previous studies on this organism led to the suggestion that the latter should originate from associated microsymbionts. In order to evaluate the influence of microsymbionts on lipid content, 10 samples of Fascaplysinopsis sp. were investigated for their sterol composition. Contrary to the secondary metabolites, the sterol patterns established were qualitatively and quantitatively stable: 14 sterols with different unsaturated nuclei, D5, D7 and D5,7, were identified; the last ones being the main sterols of the investigated sponges. The chemotaxonomic significance of these results for the order Dictyoceratida is also discussed in the context of the literature. The conjugated diene system in D5,7 sterols is known to be unstable and easily photo-oxidized during storage and/or experiments to produce 5a,8a-epidioxy sterols. However, in this study, no 5a,8a-epidioxysterols (or only trace amounts were observed. Thus, it was supposed that photo-oxidation was avoided thanks to the natural antioxidants detected in Fascaplysinopsis sp. by both the DPPH and b-caroten bleaching assays.

  6. Schmidt. Sinfonie Nr. 1 E-Dur; Strauss. Vier sinfonische Zwischenspiele aus Intermezzo. Detroit Symphony Orchestra, Neeme Järvi / Helge Grünewald

    Index Scriptorium Estoniae

    Grünewald, Helge

    1996-01-01

    Uuest heliplaadist "Schmidt. Sinfonie Nr. 1 E-Dur; Strauss. Vier sinfonische Zwischenspiele aus Intermezzo. Detroit Symphony Orchestra, Neeme Järvi. Chandos/Koch CD 9357 (WD: 68'20") DDD (WD:114'36")

  7. Syrian hamsters (Mesocricetus auratus) with simultaneous intestinal Giardia sp., Spironucleus sp., and trichomonad infections.

    Science.gov (United States)

    Sheppard, Barbara J; Stockdale Walden, Heather D; Kondo, Hirotaka

    2013-11-01

    A commercial facility producing hamsters with a history of infection by dwarf tapeworm (Hymenolepis nana) submitted 15 animals for necropsy and postmortem parasitological and microscopic examination. No tapeworms were detected grossly or microscopically. Fecal examination including gastrointestinal mucosal smears demonstrated mixed intestinal bacteria and low numbers of Giardia sp. Histologic examination of small intestine demonstrated filling of the small intestinal crypts by large numbers of 7-9 µm × 3 µm, rod to crescent or teardrop-shaped flagellates consistent with Spironucleus sp. These organisms had two 1-µm, basophilic, oval nuclei and multiple superficial flagella-like structures. Much larger 10-15 µm × 8-10 µm, oval to pear-shaped organisms were also present in lower numbers and usually located with the crypts. These larger flagellates had multiple flagella and a basophilic rod-shaped nucleus. The larger flagellates included Giardia sp., which had an intimate interface with the surface of the mucosal epithelium, bilaterally symmetry, and binucleation. Lower numbers of trichomonads were also present and were distinguished by an undulating surface membrane and a single nucleus. The mucosa was hyperplastic and moderately inflamed. Although the tapeworm infection was resolved, diagnosis of multiple intestinal flagellates by fecal examination is complicated by the varying sensitivity and diagnostic accuracy of different types of fecal analysis for different flagellate types. Key differences in the morphology and location of the different types of flagellates as observed by histology of intestinal tissues provide important additional diagnostic information to distinguish trichomonads, Spironucleus sp., and Giardia sp.

  8. Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov. prevail in diverse enzootic transmission cycles

    Science.gov (United States)

    Margos, Gabriele; Lane, Robert S.; Fedorova, Natalia; Koloczek, Johannes; Piesman, Joseph; Hojgaard, Andrias; Sing, Andreas; Fingerle, Volker

    2018-01-01

    Two species of the genus Borrelia, Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov., were first described by Postic and co-workers on the basis of genetic analyses of several loci. Multilocus sequence analysis of eight housekeeping loci confirmed that these two Borrelia genomospecies are distinct members of the Borrelia burgdorferi sensu lato complex. B. bissettiae sp. nov. was initially described in transmission cycles involving Neotoma fuscipes wood rats and Ixodes pacificus ticks in California, and Neotoma mexicana and Ixodes spinipalpis in Colorado. The preferred host of B. californiensis sp. nov. appears to be the California kangaroo rat, Dipodomys californicus; Ixodes jellisoni, I. spinipalipis and I. pacificus ticks are naturally infected with it. Thus, the ecological associations of the two genomospecies and their genetic distance from all other known Borrelia genomospecies species justify their description as separate genomospecies: B. bissettiae sp. nov. (type strain DN127T=DSM 17990T= CIP 109136T) and B. californiensis (type strain CA446T=DSM 17989T=ATCC BAA-2689T). PMID:26813420

  9. The novel fusion transcript NR5A2-KLHL29FT is generated by an insertion at the KLHL29 locus.

    Science.gov (United States)

    Sun, Zhenguo; Ke, Xiquan; Salzberg, Steven L; Kim, Daehwan; Antonescu, Valentin; Cheng, Yulan; Huang, Binbin; Song, Jee Hoon; Abraham, John M; Ibrahim, Sariat; Tian, Hui; Meltzer, Stephen J

    2017-05-01

    Novel fusion transcripts (FTs) caused by chromosomal rearrangement are common factors in the development of cancers. In the current study, the authors used massively parallel RNA sequencing to identify new FTs in colon cancers. RNA sequencing (RNA-Seq) and TopHat-Fusion were used to identify new FTs in colon cancers. The authors then investigated whether the novel FT nuclear receptor subfamily 5, group A, member 2 (NR5A2)-Kelch-like family member 29 FT (KLHL29FT) was transcribed from a genomic chromosomal rearrangement. Next, the expression of NR5A2-KLHL29FT was measured by quantitative real-time polymerase chain reaction in colon cancers and matched corresponding normal epithelia. The authors identified the FT NR5A2-KLHL29FT in normal and cancerous epithelia. While investigating this transcript, it was unexpectedly found that it was due to an uncharacterized polymorphic germline insertion of the NR5A2 sequence from chromosome 1 into the KLHL29 locus at chromosome 2, rather than a chromosomal rearrangement. This germline insertion, which occurred at a population frequency of 0.40, appeared to bear no relationship to cancer development. Moreover, expression of NR5A2-KLHL29FT was validated in RNA specimens from samples with insertions of NR5A2 at the KLHL29 gene locus, but not from samples without this insertion. It is interesting to note that NR5A2-KLH29FT expression levels were significantly lower in colon cancers than in matched normal colonic epithelia (P =.029), suggesting the potential participation of NR5A2-KLHL29FT in the origin or progression of this tumor type. NR5A2-KLHL29FT was generated from a polymorphism insertion of the NR5A2 sequence into the KLHL29 locus. NR5A2-KLHL29FT may influence the origin or progression of colon cancer. Moreover, researchers should be aware that similar FTs may occur due to transchromosomal insertions that are not correctly annotated in genome databases, especially with current assembly algorithms. Cancer 2017;123:1507-1515.

  10. NCBI nr-aa BLAST: CBRC-GACU-23-0013 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GACU-23-0013 gb|AAV93255.1| putative green sensitive visual pigment [Pundamili...a nyererei] gb|AAV93258.1| putative green sensitive visual pigment [Pundamilia sp. 'luanso'] gb|AAV93259.1| ...putative green sensitive visual pigment [Pundamilia sp. 'red head'] gb|AAV93260.1| putative green sensitive visual... pigment [Lipochromis melanopterus] gb|AAW34315.1| putative green sensitive visual... pigment [Pundamilia nyererei] gb|AAW34316.1| putative green sensitive visual pigment [Pundamilia nye

  11. EFEKTIFITAS DAYA HAMBAT BAKTERI Streptomyces sp TERHADAP Erwinia sp PENYEBAB PENYAKIT BUSUK REBAH PADA TANAMAN LIDAH BUAYA (Aloe barbadensis Mill

    Directory of Open Access Journals (Sweden)

    SARMILA TASNIM

    2013-05-01

    Full Text Available Streptomyces sp was conducted from December 2010 - June 2011 at the Laboratoryof Microbiology, Biology Department, Math and Science Faculty, UdayanaUniversity Bukit Jimbaran-Bali. Implementation stages of the research consisted ofisolation and testing of the antibiotic activity Streptomyces sp to inhibit growthbacterial pathogens Erwinia sp as a cause of disease in plants fallen foul (Soft rot ofAloe barbadensis Mill.The results of this study have eight isolates of Streptomyces spwith macroscopic and microscopic characters are varied. Furthermore, all isolateswere obtained and then tested against antibiotic activity to inhibit growth the bacteriaErwinia sp. Test results obtained by Streptomyces sp that has the most effective ininhibiting the ability of the bacteria Erwinia sp isolates are Streptomyces sp2for (45%.

  12. Effects of a wide range of dietary nicotinamide riboside (NR) concentrations on metabolic flexibility and white adipose tissue (WAT) of mice fed a mildly obesogenic diet.

    Science.gov (United States)

    Shi, Wenbiao; Hegeman, Maria A; van Dartel, Dorien A M; Tang, Jing; Suarez, Manuel; Swarts, Hans; van der Hee, Bart; Arola, Lluis; Keijer, Jaap

    2017-08-01

    Metabolic flexibility is the ability to switch metabolism between carbohydrate oxidation (CHO) and fatty acid oxidation (FAO) and is a biomarker for metabolic health. The effect on metabolic health of nicotinamide riboside (NR) as an exclusive source of vitamin B3 is unknown and is examined here for a wide range of NR. Nine-week-old male C57BL/6JRcc mice received a semi-purified mildly obesogenic (40 en% fat) diet containing 0.14% L-tryptophan and either 5, 15, 30, 180, or 900 mg NR per kg diet for 15 weeks. Body composition and metabolic parameters were analyzed. Metabolic flexibility was measured using indirect calorimetry. Gene expression in epididymal white adipose tissue (eWAT) was measured using qRT-PCR . The maximum delta respiratory exchange ratio when switching from CHO to FAO (maxΔRER CHO1→FAO ) and when switching from FAO to CHO (maxΔRER FAO→CHO2 ) were largest in 30 mg NR per kg diet (30NR). In eWAT, the gene expression of Pparγ, a master regulator of adipogenesis, and of Sod2 and Prdx3, two antioxidant genes, were significantly upregulated in 30NR compared to 5NR. 30NR is most beneficial for metabolic health, in terms of metabolic flexibility and eWAT gene expression, of mice on an obesogenic diet. © 2017 The Authors. Molecular Nutrition & Food Research published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. The qEEG Signature of Selective NMDA NR2B Negative Allosteric Modulators; A Potential Translational Biomarker for Drug Development.

    Directory of Open Access Journals (Sweden)

    Deborah Keavy

    Full Text Available The antidepressant activity of the N-methyl-D-aspartate (NMDA receptor channel blocker, ketamine, has led to the investigation of negative allosteric modulators (NAMs selective for the NR2B receptor subtype. The clinical development of NR2B NAMs would benefit from a translational pharmacodynamic biomarker that demonstrates brain penetration and functional inhibition of NR2B receptors in preclinical species and humans. Quantitative electroencephalography (qEEG is a translational measure that can be used to demonstrate pharmacodynamic effects across species. NMDA receptor channel blockers, such as ketamine and phencyclidine, increase the EEG gamma power band, which has been used as a pharmacodynamic biomarker in the development of NMDA receptor antagonists. However, detailed qEEG studies with ketamine or NR2B NAMs are lacking in nonhuman primates. The aim of the present study was to determine the effects on the qEEG power spectra of the NR2B NAMs traxoprodil (CP-101,606 and BMT-108908 in nonhuman primates, and to compare them to the NMDA receptor channel blockers, ketamine and lanicemine. Cynomolgus monkeys were surgically implanted with EEG radio-telemetry transmitters, and qEEG was measured after vehicle or drug administration. The relative power for a number of frequency bands was determined. Ketamine and lanicemine increased relative gamma power, whereas the NR2B NAMs traxoprodil and BMT-108908 had no effect. Robust decreases in beta power were elicited by ketamine, traxoprodil and BMT-108908; and these agents also produced decreases in alpha power and increases in delta power at the doses tested. These results suggest that measurement of power spectra in the beta and delta bands may represent a translational pharmacodynamic biomarker to demonstrate functional effects of NR2B NAMs. The results of these studies may help guide the selection of qEEG measures that can be incorporated into early clinical evaluation of NR2B NAMs in healthy humans.

  14. Integrative proteomic analysis of the NMDA NR1 knockdown mouse model reveals effects on central and peripheral pathways associated with schizophrenia and autism spectrum disorders

    OpenAIRE

    Wesseling, Hendrik; Guest, Paul; Lee, Chi-Ming; Wong, Erik; Rahmoune, Hassan; Bahn, Sabine

    2014-01-01

    textabstractBackground: Over the last decade, the transgenic N-methyl-D-aspartate receptor (NMDAR) NR1-knockdown mouse (NR1neo-/-) has been investigated as a glutamate hypofunction model for schizophrenia. Recent research has now revealed that the model also recapitulates cognitive and negative symptoms in the continuum of other psychiatric diseases, particularly autism spectrum disorders (ASD). As previous studies have mostly focussed on behavioural readouts, a molecular characterisation of ...

  15. Testosterone production during puberty in two 46,XY patients with disorders of sex development and novel NR5A1 (SF-1) mutations.

    Science.gov (United States)

    Tantawy, Sally; Lin, Lin; Akkurt, Ilker; Borck, Guntram; Klingmüller, Dietrich; Hauffa, Berthold P; Krude, Heiko; Biebermann, Heike; Achermann, John C; Köhler, Birgit

    2012-07-01

    Steroidogenic factor 1 (SF-1, NR5A1) is a key transcriptional regulator of many genes involved in the hypothalamic-pituitary-gonadal axis and mutations in NR5A1 can result in 46,XY disorders of sex development (DSD). Patients with this condition typically present with ambiguous genitalia, partial gonadal dysgenesis, and absent/rudimentary Müllerian structures. In these cases, testosterone is usually low in early infancy, indicating significantly impaired androgen synthesis. Further, Sertoli cell dysfunction is seen (low inhibin B, anti-Müllerian hormone). However, gonadal function at puberty in patients with NR5A1 mutations is unknown. Clinical assessment, endocrine evaluation, and genetic analysis were performed in one female and one male with 46,XY DSD who showed spontaneous virilization during puberty. The female patient presented at adolescence with clitoral hypertrophy, whereas the male patient presented at birth with severe hypospadias and entered puberty spontaneously. Molecular analysis of NR5A1 was performed followed by in vitro functional analysis of the two novel mutations detected. Testosterone levels were normal during puberty in both patients. Analysis of NR5A1 revealed two novel heterozygous missense mutations in the ligand-binding domain of SF-1 (patient 1: p.L376F; patient 2: p.G328V). The mutant proteins showed reduced transactivation of the CYP11A promoter in vitro. Patients with 46,XY DSD and NR5A1 mutations can produce sufficient testosterone for spontaneous virilization during puberty. Phenotypic females (46,XY) with NR5A1 mutations can present with clitoromegaly at puberty, a phenotype similar to other partial defects of androgen synthesis or action. Testosterone production in 46,XY males with NR5A1 mutations can be sufficient for virilization at puberty. As progressive gonadal dysgenesis is likely, gonadal function should be monitored in adolescence and adulthood, and early sperm cryopreservation considered in male patients if possible.

  16. SpAHA1 and SpSOS1 Coordinate in Transgenic Yeast to Improve Salt Tolerance.

    Directory of Open Access Journals (Sweden)

    Yang Zhou

    Full Text Available In plant cells, the plasma membrane Na+/H+ antiporter SOS1 (salt overly sensitive 1 mediates Na+ extrusion using the proton gradient generated by plasma membrane H+-ATPases, and these two proteins are key plant halotolerance factors. In the present study, two genes from Sesuvium portulacastrum, encoding plasma membrane Na+/H+ antiporter (SpSOS1 and H+-ATPase (SpAHA1, were cloned. Localization of each protein was studied in tobacco cells, and their functions were analyzed in yeast cells. Both SpSOS1 and SpAHA1 are plasma membrane-bound proteins. Real-time polymerase chain reaction (PCR analyses showed that SpSOS1 and SpAHA1 were induced by salinity, and their expression patterns in roots under salinity were similar. Compared with untransformed yeast cells, SpSOS1 increased the salt tolerance of transgenic yeast by decreasing the Na+ content. The Na+/H+ exchange activity at plasma membrane vesicles was higher in SpSOS1-transgenic yeast than in the untransformed strain. No change was observed in the salt tolerance of yeast cells expressing SpAHA1 alone; however, in yeast transformed with both SpSOS1 and SpAHA1, SpAHA1 generated an increased proton gradient that stimulated the Na+/H+ exchange activity of SpSOS1. In this scenario, more Na+ ions were transported out of cells, and the yeast cells co-expressing SpSOS1 and SpAHA1 grew better than the cells transformed with only SpSOS1 or SpAHA1. These findings demonstrate that the plasma membrane Na+/H+ antiporter SpSOS1 and H+-ATPase SpAHA1 can function in coordination. These results provide a reference for developing more salt-tolerant crops via co-transformation with the plasma membrane Na+/H+ antiporter and H+-ATPase.

  17. Synergistic effect of nano calcium carbonate (NCC)/carbon black (CB) on the cure characteristics and physico-mechanical properties of NR/SBR blends

    Science.gov (United States)

    Mamauod, Siti Nur Liyana; Romli, Ahmad Zafir; Rizuan, Mohd Ismail Rifdi

    2017-09-01

    This research was carried out as to develop hybrid filler reinforced into the blend of natural rubber (NR) and styrene butadiene rubber (SBR). The NR/SBR blend was reinforced using carbon black (CB) and nano calcium carbonate (NCC). The NCC content varied from 2-10 phr which was incorporated into the NR/SBR blend filled with fixed 50 phr of CB. The main aim of this project was to study the synergistic effect of NCC and CB reinforced NR/SBR blends towards the curing characteristics using cure rheometer, the viscosity of uncured NR/SBR compounds, physical and mechanical property blends. From the results obtained, the optimum ratio of blending was identified at 4 phr of NCC loading. Tensile strength, elongation at break, modulus and hardness increased progressively with increasing the NCC loading from 0 phr up to a maximum value at 4 phr. This increment occurs due to consolidation of the network structure of the polymer chains with the increasing NCC content. Up to the optimum amount of NCC, the tendency for NCC particles to form aggregate was very high and hence reduces the properties of rubber blends. It proved that NCC acts as a co-reinforcing agent for CB to improve the performance in the NR/SBR blends.

  18. Leptin reverses corticosterone-induced inhibition of neural stem cell proliferation through activating the NR2B subunits of NMDA receptors

    Energy Technology Data Exchange (ETDEWEB)

    Shi, Wen-Zhu [Anesthesia and Operation Center, Hainan Branch of Chinese PLA General Hospital, Hainan 572013 (China); Anesthesia and Operation Center, Chinese PLA General Hospital, Beijing 100853 (China); Miao, Yu-Liang [Department of Anesthesiology, PLA No. 306 Hospital, Beijing 100101 (China); Guo, Wen-Zhi [Department of Anesthesiology, Beijing Military General Hospital of Chinese People’s Liberation Army, Beijing 100700 (China); Wu, Wei, E-mail: wwzwgk@163.com [Department of Head and Neck Surgery of Otolaryngology, PLA No. 306 Hospital, Beijing 100101 (China); Li, Bao-Wei [Department of Head and Neck Surgery of Otolaryngology, PLA No. 306 Hospital, Beijing 100101 (China); An, Li-Na [Department of Anesthesiology, Armed Police General Hospital, Beijing 100039 (China); Fang, Wei-Wu [Department of Anesthesiology, PLA No. 306 Hospital, Beijing 100101 (China); Mi, Wei-Dong, E-mail: elite2005gg@163.com [Anesthesia and Operation Center, Chinese PLA General Hospital, Beijing 100853 (China)

    2014-04-25

    Highlights: • Leptin promotes the proliferation of neural stem cells isolated from embryonic mouse hippocampus. • Leptin reverses corticosterone-induced inhibition of neural stem cell proliferation. • The effects of leptin are partially mediated by upregulating NR2B subunits. - Abstract: Corticosterone inhibits the proliferation of hippocampal neural stem cells (NSCs). The removal of corticosterone-induced inhibition of NSCs proliferation has been reported to contribute to neural regeneration. Leptin has been shown to regulate brain development, improve angiogenesis, and promote neural regeneration; however, its effects on corticosterone-induced inhibition of NSCs proliferation remain unclear. Here we reported that leptin significantly promoted the proliferation of hippocampal NSCs in a concentration-dependent pattern. Also, leptin efficiently reversed the inhibition of NSCs proliferation induced by corticosterone. Interestingly, pre-treatment with non-specific NMDA antagonist MK-801, specific NR2B antagonist Ro 25-6981, or small interfering RNA (siRNA) targeting NR2B, significantly blocked the effect of leptin on corticosterone-induced inhibition of NSCs proliferation. Furthermore, corticosterone significantly reduced the protein expression of NR2B, whereas pre-treatment with leptin greatly reversed the attenuation of NR2B expression caused by corticosterone in cultured hippocampal NSCs. Our findings demonstrate that leptin reverses the corticosterone-induced inhibition of NSCs proliferation. This process is, at least partially mediated by increased expression of NR2B subunits of NMDA receptors.

  19. Effect of combination ultrasonic and ball milling techniques of commercial fillers dispersion on mechanical properties of natural rubber (NR) latex films

    Science.gov (United States)

    Hamran, Noramirah; Rashid, Azura A.

    2017-07-01

    Commercial fillers such as silica and carbon black generally impart the reinforcing effects in dry rubber compound, but have an adverse effect on Natural rubber (NR) latex compounds. The addition of commercial fillers in NR latex has reduced the mechanical properties of NR latex films due to the destabilization effect in the NR latex compounds which govern by the dispersion quality, particle size and also the pH of the dispersion itself. The ball milling process is the conventional meth od of preparation of dispersions and ultrasonic has successfully used in preparation of nano fillers such as carbon nanotube (CNT). In this study the combination between the conventional methods; ball milling together the ultrasonic method were used to prepare the silica and carbon black dispersions. The different duration of ball milling (24, 48 and 72 hours) was compared with the ultrasonic method (30, 60, 90 and 120 minutes). The combination of ball milling and ultrasonic from the optimum individual technique was used to investigate the reduction of particle size of the fillers. The particle size analyzer, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) test were carried out to investigate the obtained particle size and the tensile and tear test were carried out to investigate the mechanical properties of the NR latex films. The reduction of filler particle size is expected to impart the properties of NR latex films.

  20. Copper resistance of different ectomycorrhizal fungi such as Pisolithus microcarpus, Pisolithus sp., Scleroderma sp. and Suillus sp.

    Directory of Open Access Journals (Sweden)

    R.F. Silva

    2013-01-01

    Full Text Available Environments contaminated with heavy metals negatively impact the living organisms. Ectomycorrhizal fungi have shown important role in these impacted sites. Thus, this study aimed to evaluate the copper-resistance of ectomycorrhizal fungi isolates Pisolithus microcarpus - UFSC-Pt116; Pisolithus sp. - UFSC-PT24, Suillus sp. - UFSM RA 2.8 and Scleroderma sp. - UFSC-Sc124 to different copper doses in solid and liquid media. The copper doses tested were: 0.00, 0.25, 0.5, 0.75, 1.0 and 1.25 mmol L-1 in the solid medium and 0.00, 0.32, 0.64 and 0.96 mmol L-1 in the liquid medium. Copper was amended as copper sulphate in order to supplement the culture medium MNM at pH 4.8, with seven replicates to each fungus-dose combination. The fungal isolates were incubated for 30 days at 28 °C. UFSC-Pt116 showed high copper-resistance such as accessed by CL50 determinations (concentration to reduce 50% of the growth as while as UFSC-PT24 displayed copper-resistance mechanism at 0.50 mmol L-1 in solid medium. The UFSC-PT24 and UFSC-Sc124 isolates have increased copper-resistance in liquid medium. The higher production of extracellular pigment was detected in UFSC-Pt116 cultures. The UFSC-Pt116 and UFSC-PT24 isolates showed higher resistance for copper and produced higher mycelium biomass than the other isolates. In this way, the isolates UFSG-Pt116 and UFSC-PT24 can be important candidates to survive in copper-contaminated areas, and can show important role in plants symbiosis in these contaminated sites.

  1. Avaliação do cumprimento da NR-18 em função do porte de obra residencial e proposta de lista de verificação da NR-18

    Directory of Open Access Journals (Sweden)

    Marcelo Fabiano Costella

    Full Text Available O presente trabalho investiga as reais condições dos ambientes de trabalho da indústria da construção, tendo em vista os problemas relacionados à segurança do trabalho nos canteiros de obras. Formulou-se a hipótese de haver discrepância no grau de cumprimento da NR-18 em obras de diferentes portes (pequena, média e grande. Baseado em uma lista de verificação do grau de cumprimento dos requisitos da NR-18, foram avaliados 115 canteiros de obra na cidade de Chapecó - SC. Para cada item da lista de verificação foi atribuída uma nota e, após compilar as informações, estas foram avaliadas conforme os objetivos da pesquisa. Fez-se uma análise crítica para os cinco piores e os cinco melhores itens observados para as obras pequenas, médias e grandes. Analisando-se os resultados é possível afirmar que há uma diferença significativa de aplicação da NR-18 nos diferentes portes de obras, sendo que as obras de grande porte obtiveram uma média final (6,47 significativamente superior em relação às obras pequenas (1,97. Desta forma, foi corroborada a hipótese formulada, podendo se atribuir este resultado a diversos fatores, entre os quais a falta de habilidades gerenciais de segurança e saúde do trabalho nas pequenas empresas. Outra contribuição deste artigo foi a proposta de uma nova lista de verificação para avaliar o grau de cumprimento da NR-18 nos canteiros de obra.

  2. Isolation of C11 Cyclopentenones from Two Didemnid Species, Lissoclinum sp. and Diplosoma sp.

    Directory of Open Access Journals (Sweden)

    Katsuhiro Ueda

    2009-12-01

    Full Text Available A series of new C11 cyclopentenones 1-7 was isolated, together with four known metabolites 9/10, 12 and 13, from the extract of the didemnid ascidian Lissoclinum sp. The other didemnid ascidian Diplosoma sp. contained didemnenones 1, 2 and 5, and five known metabolites 8-12. The structures of 1-7 were elucidated by spectroscopic analyses. Cytotoxicity of the isolated compounds was evaluated against three human cancer cell lines (HCT116, A431 and A549.

  3. Construction of human MASP-2-CCP1/2SP, CCP2SP, SP plasmid DNA nanolipoplexes and the effects on tuberculosis in BCG-infected mice.

    Science.gov (United States)

    Gao, Qi; Dong, Xinfang; Luo, Yanping; Zhang, Guochao; Shan, Jinyu; Wang, Qian; He, Qi; Zhang, Lifeng; Wang, Jingqiu; Zhu, Bingdong; Ma, Xingming

    2017-08-01

    The lectin pathway, one of the complement cascade systems, provides the primary line of defense against invading pathogens. The serine protease of MASP-2 plays an essential role in complement activation of the lectin pathway. The C-terminal segment of MASP-2 is comprised of the CCP1-CCP2-SP domains, and is the crucial catalytic segment. However, what is the effect of CCP1-CCP2-SP domains in controlling chronic infection is unknown. In order to evaluate the potential impact of CCP1-CCP2-SP domains on tuberculosis, we constructed the human MASP-2 CCP1/2SP, CCP2SP and SP recombinant plasmids, and delivered these plasmids by DNA-DOTAP:cholesterol cationic nanolipoplexes to BCG-infected mice. After 21 days post DNA-DOTAP:chol nanolipoplexes application, we analyzed bacteria loads of pulmonary, pathology of granuloma, lymphocyte subpopulations. The C3a, C4a and MASP-2 levels in serum were measured with enzyme-linked immunosorbent assays. Compared to the control group that received GFP DNA-DOTAP:chol nanolipoplexes, MASP-2 CCP1/2SP DNA-DOTAP:chol nanolipoplexes treated group showed significantly enlarged pulmonary granulomas lesion (P  0.05). These findings provided experimental evidence that MASP-2 CCP1/2SP DNA nanolipoplexes shown the negative efficacy in controlling Mycobacterium tuberculosis infection, and displayed a potential role of down-regulating T-cell-mediated immunity in tuberculosis. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. NCBI nr-aa BLAST: CBRC-DNOV-01-2774 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-2774 sp|Q9QZD5|RAE2_MOUSE Rab proteins geranylgeranyltransferase compo...nent A 2 (Rab escort protein 2) (REP-2) (Choroideraemia-like protein) gb|AAI18933.1| Choroideremia-like [Mus musculus] Q9QZD5 3e-44 82% ...

  5. NCBI nr-aa BLAST: CBRC-DSIM-02-0039 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DSIM-02-0039 ref|NP_001083797.1| cysteine-string protein [Xenopus laevis] sp|O...42196|CSP_XENLA Cysteine string protein (CSP) (Xcsp) gb|AAB69692.1| cysteine-string protein [Xenopus laevis] NP_001083797.1 0.046 46% ...

  6. NCBI nr-aa BLAST: CBRC-FRUB-02-0858 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FRUB-02-0858 sp|P41755|DHE2_ACHKL NAD-specific glutamate dehydrogenase (NAD-GDH) pir||A53164 glutamate... dehydrogenase (EC 1.4.1.2) - Achlya klebsiana gb|AAA17563.1| NAD-specific glutamate dehydrogenase P41755 3e-45 35% ...

  7. NCBI nr-aa BLAST: CBRC-OLAT-08-0000 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OLAT-08-0000 sp|P41755|DHE2_ACHKL NAD-specific glutamate dehydrogenase (NAD-GDH) pir||A53164 glutamate... dehydrogenase (EC 1.4.1.2) - Achlya klebsiana gb|AAA17563.1| NAD-specific glutamate dehydrogenase P41755 2e-83 36% ...

  8. NCBI nr-aa BLAST: CBRC-ACAR-01-0732 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-0732 sp|P21109|CXCR1_RABIT High affinity interleukin-8 receptor A (IL-...8R A) (CXCR-1) (CD181 antigen) gb|AAA31375.1| interleukin 8 receptor gb|AAA31376.1| interleukin 8 receptor P21109 1e-113 64% ...

  9. NCBI nr-aa BLAST: CBRC-TBEL-01-0817 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TBEL-01-0817 sp|P21109|CXCR1_RABIT High affinity interleukin-8 receptor A (IL-...8R A) (CXCR-1) (CD181 antigen) gb|AAA31375.1| interleukin 8 receptor gb|AAA31376.1| interleukin 8 receptor P21109 1e-134 68% ...

  10. NCBI nr-aa BLAST: CBRC-EEUR-01-0272 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-EEUR-01-0272 sp|P21109|CXCR1_RABIT High affinity interleukin-8 receptor A (IL-...8R A) (CXCR-1) (CD181 antigen) gb|AAA31375.1| interleukin 8 receptor gb|AAA31376.1| interleukin 8 receptor P21109 1e-141 72% ...

  11. NCBI nr-aa BLAST: CBRC-CPOR-01-0033 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CPOR-01-0033 sp|P21109|CXCR1_RABIT High affinity interleukin-8 receptor A (IL-...8R A) (CXCR-1) (CD181 antigen) gb|AAA31375.1| interleukin 8 receptor gb|AAA31376.1| interleukin 8 receptor P21109 9e-64 80% ...

  12. NCBI nr-aa BLAST: CBRC-OANA-01-0052 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OANA-01-0052 sp|P21109|CXCR1_RABIT High affinity interleukin-8 receptor A (IL-...8R A) (CXCR-1) (CD181 antigen) gb|AAA31375.1| interleukin 8 receptor gb|AAA31376.1| interleukin 8 receptor P21109 1e-114 63% ...

  13. NCBI nr-aa BLAST: CBRC-OCUN-01-0050 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OCUN-01-0050 sp|P21109|CXCR1_RABIT High affinity interleukin-8 receptor A (IL-...8R A) (CXCR-1) (CD181 antigen) gb|AAA31375.1| interleukin 8 receptor gb|AAA31376.1| interleukin 8 receptor P21109 1e-164 81% ...

  14. NCBI nr-aa BLAST: CBRC-CFAM-37-0005 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CFAM-37-0005 sp|P21109|CXCR1_RABIT High affinity interleukin-8 receptor A (IL-...8R A) (CXCR-1) (CD181 antigen) gb|AAA31375.1| interleukin 8 receptor gb|AAA31376.1| interleukin 8 receptor P21109 1e-152 76% ...

  15. NCBI nr-aa BLAST: CBRC-OANA-01-1436 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OANA-01-1436 sp|P21109|CXCR1_RABIT High affinity interleukin-8 receptor A (IL-...8R A) (CXCR-1) (CD181 antigen) gb|AAA31375.1| interleukin 8 receptor gb|AAA31376.1| interleukin 8 receptor P21109 1e-115 59% ...

  16. NCBI nr-aa BLAST: CBRC-RNOR-09-0076 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-09-0076 sp|P21109|CXCR1_RABIT High affinity interleukin-8 receptor A (IL-...8R A) (CXCR-1) (CD181 antigen) gb|AAA31375.1| interleukin 8 receptor gb|AAA31376.1| interleukin 8 receptor P21109 1e-127 69% ...

  17. NCBI nr-aa BLAST: CBRC-MMUS-01-0026 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-01-0026 sp|P21109|CXCR1_RABIT High affinity interleukin-8 receptor A (IL-...8R A) (CXCR-1) (CD181 antigen) gb|AAA31375.1| interleukin 8 receptor gb|AAA31376.1| interleukin 8 receptor P21109 1e-127 70% ...

  18. NCBI nr-aa BLAST: CBRC-BTAU-01-2915 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-BTAU-01-2915 sp|P21109|CXCR1_RABIT High affinity interleukin-8 receptor A (IL-...8R A) (CXCR-1) (CD181 antigen) gb|AAA31375.1| interleukin 8 receptor gb|AAA31376.1| interleukin 8 receptor P21109 1e-137 72% ...

  19. NCBI nr-aa BLAST: CBRC-OANA-01-2186 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OANA-01-2186 ref|NP_001618.2| activated leukocyte cell adhesion molecule [Homo... sapiens] sp|Q13740|CD166_HUMAN CD166 antigen precursor (Activated leukocyte-cell adhesion molecule) (ALCAM)... gb|ABF48406.1| activated leukocyte cell adhesion molecule variant 2 [Homo sapiens] NP_001618.2 5.1 29% ...

  20. NCBI nr-aa BLAST: CBRC-RMAC-03-0036 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RMAC-03-0036 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 1e-138 72% ...

  1. NCBI nr-aa BLAST: CBRC-DRER-12-0072 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DRER-12-0072 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 1e-119 57% ...

  2. NCBI nr-aa BLAST: CBRC-HSAP-07-0017 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-HSAP-07-0017 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 0.0 86% ...

  3. NCBI nr-aa BLAST: CBRC-ACAR-01-0764 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-0764 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 1e-141 56% ...

  4. NCBI nr-aa BLAST: CBRC-TGUT-04-0018 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TGUT-04-0018 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 1e-147 60% ...

  5. NCBI nr-aa BLAST: CBRC-BTAU-01-2615 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-BTAU-01-2615 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 0.0 82% ...

  6. NCBI nr-aa BLAST: CBRC-OLAT-04-0021 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OLAT-04-0021 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 1e-110 52% ...

  7. NCBI nr-aa BLAST: CBRC-DNOV-01-2844 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-2844 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 1e-126 58% ...

  8. NCBI nr-aa BLAST: CBRC-EEUR-01-0660 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-EEUR-01-0660 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 1e-129 83% ...

  9. NCBI nr-aa BLAST: CBRC-CPOR-01-2037 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CPOR-01-2037 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 1e-110 73% ...

  10. NCBI nr-aa BLAST: CBRC-GGAL-02-0000 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GGAL-02-0000 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 1e-148 58% ...

  11. NCBI nr-aa BLAST: CBRC-FRUB-02-0138 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FRUB-02-0138 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 1e-120 54% ...

  12. NCBI nr-aa BLAST: CBRC-PABE-08-0029 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PABE-08-0029 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 0.0 80% ...

  13. NCBI nr-aa BLAST: CBRC-LAFR-01-0274 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-0274 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 1e-106 84% ...

  14. NCBI nr-aa BLAST: CBRC-CJAC-01-1513 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-1513 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 0.0 80% ...

  15. NCBI nr-aa BLAST: CBRC-FCAT-01-0862 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-0862 ref|NP_999200.1| growth hormone releasing hormone receptor [Sus s...crofa] sp|P34999|GHRHR_PIG Growth hormone-releasing hormone receptor precursor (GHRH receptor) (GRF receptor...) (GRFR) gb|AAA93391.1| growth hormone-releasing hormone receptor NP_999200.1 1e-179 79% ...

  16. NCBI nr-aa BLAST: CBRC-AGAM-02-0067 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-02-0067 sp|Q8BZH4|POGZ_MOUSE Pogo transposable element with ZNF domain gb...|AAH96492.1| Pogo transposable element with ZNF domain [Mus musculus] gb|EDL38753.1| pogo transposable element with ZNF domain, isoform CRA_c [Mus musculus] Q8BZH4 5.3 25% ...

  17. NCBI nr-aa BLAST: CBRC-FRUB-02-0631 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FRUB-02-0631 sp|Q9XTA3|MYOC_BOVIN Myocilin precursor (Trabecular meshwork-indu...ced glucocorticoid response protein) dbj|BAA82152.1| myocilin/trabecular meshwork inducible glucocorticoid response protein [Bos taurus] Q9XTA3 1e-101 52% ...

  18. NCBI nr-aa BLAST: CBRC-GACU-03-0016 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GACU-03-0016 sp|Q9XTA3|MYOC_BOVIN Myocilin precursor (Trabecular meshwork-indu...ced glucocorticoid response protein) dbj|BAA82152.1| myocilin/trabecular meshwork inducible glucocorticoid response protein [Bos taurus] Q9XTA3 1e-114 50% ...

  19. NCBI nr-aa BLAST: CBRC-DYAK-03-0005 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DYAK-03-0005 ref|ZP_01001263.1| copper-translocating P-type ATPase [Oceanicola bats...01368.1| copper-translocating P-type ATPase [Oceanicola batsensis HTCC2597] gb|EAU47853.1| copper-translocating P-type ATPase [Roseovarius sp. HTCC2601] ZP_01001263.1 0.16 26% ...

  20. NCBI nr-aa BLAST: CBRC-EEUR-01-1329 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-EEUR-01-1329 sp|P16112|PGCA_HUMAN Aggrecan core protein precursor (Cartilage-specific proteoglycan core... protein) (CSPCP) (Chondroitin sulfate proteoglycan core protein 1) [Contains: Aggrecan core protein 2] P16112 4e-16 20% ...

  1. NCBI nr-aa BLAST: CBRC-TTRU-01-1120 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-1120 sp|Q86UP3|ZFHX4_HUMAN RecName: Full=Zinc finger homeobox protein ...4; AltName: Full=Zinc finger homeodomain protein 4; Short=ZFH-4 gb|AAP20225.1| zinc finger homeodomain 4 protein [Homo sapiens] Q86UP3 0.0 87% ...

  2. NCBI nr-aa BLAST: CBRC-PVAM-01-1002 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PVAM-01-1002 sp|Q86UP3|ZFHX4_HUMAN RecName: Full=Zinc finger homeobox protein ...4; AltName: Full=Zinc finger homeodomain protein 4; Short=ZFH-4 gb|AAP20225.1| zinc finger homeodomain 4 protein [Homo sapiens] Q86UP3 0.0 86% ...

  3. NCBI nr-aa BLAST: CBRC-OPRI-01-1361 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OPRI-01-1361 sp|Q86UP3|ZFHX4_HUMAN RecName: Full=Zinc finger homeobox protein ...4; AltName: Full=Zinc finger homeodomain protein 4; Short=ZFH-4 gb|AAP20225.1| zinc finger homeodomain 4 protein [Homo sapiens] Q86UP3 0.0 93% ...

  4. NCBI nr-aa BLAST: CBRC-MDOM-03-0053 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MDOM-03-0053 sp|Q86UP3|ZFHX4_HUMAN RecName: Full=Zinc finger homeobox protein ...4; AltName: Full=Zinc finger homeodomain protein 4; Short=ZFH-4 gb|AAP20225.1| zinc finger homeodomain 4 protein [Homo sapiens] Q86UP3 0.0 94% ...

  5. NCBI nr-aa BLAST: CBRC-PABE-03-0001 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PABE-03-0001 ref|NP_001017603.1| N-acylsphingosine amidohydrolase 3 [Danio rer...io] sp|Q568I2|ASAH3_BRARE Alkaline ceramidase 1 (Alkaline CDase-1) (AlkCDase 1) (Acylsphingosine deacylase 3) (N-acylsphingos

  6. NCBI nr-aa BLAST: CBRC-DNOV-01-1957 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-1957 ref|NP_031461.1| ATP-binding cassette, sub-family D, member 1 [Mus musculus] sp|P48410|ABC...D1_MOUSE ATP-binding cassette sub-family D member 1 (Adrenoleukodystrophy protein)

  7. NCBI nr-aa BLAST: CBRC-MEUG-01-1506 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available musculus] sp|Q8BX46|LBXCO_MOUSE RecName: Full=Ladybird homeobox corepressor 1; AltName: Full=Lbx1 corepress...or 1; AltName: Full=Transcriptional corepressor Corl1; AltName: Full=Fussel-15 gb|EDL26034.1| ladybird homeo

  8. NCBI nr-aa BLAST: CBRC-PTRO-19-0001 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PTRO-19-0001 sp|Q27409|FP1_MYTGA Adhesive plaque matrix protein precursor (Foo...t protein 1) (MGFP1) (MGFP-1) dbj|BAA09851.1| adhesive plaque protein [Mytilus galloprovincialis] Q27409 3e-07 25% ...

  9. NCBI nr-aa BLAST: CBRC-CJAC-01-1221 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-1221 sp|Q25434|FP1_MYTCO Adhesive plaque matrix protein precursor (Foo...t protein 1) (MCFP1) dbj|BAA09850.1| adhesive plaque matrix protein [Mytilus coruscus] Q25434 3e-11 24% ...

  10. NCBI nr-aa BLAST: CBRC-CJAC-01-1221 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-1221 sp|Q27409|FP1_MYTGA Adhesive plaque matrix protein precursor (Foo...t protein 1) (MGFP1) (MGFP-1) dbj|BAA09851.1| adhesive plaque protein [Mytilus galloprovincialis] Q27409 2e-26 30% ...

  11. NCBI nr-aa BLAST: CBRC-PTRO-19-0001 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PTRO-19-0001 sp|Q25434|FP1_MYTCO Adhesive plaque matrix protein precursor (Foo...t protein 1) (MCFP1) dbj|BAA09850.1| adhesive plaque matrix protein [Mytilus coruscus] Q25434 4e-08 22% ...

  12. NCBI nr-aa BLAST: CBRC-HSAP-02-0042 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-HSAP-02-0042 sp|Q25434|FP1_MYTCO Adhesive plaque matrix protein precursor (Foo...t protein 1) (MCFP1) dbj|BAA09850.1| adhesive plaque matrix protein [Mytilus coruscus] Q25434 1e-30 28% ...

  13. NCBI nr-aa BLAST: CBRC-XTRO-01-3861 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-3861 sp|Q27409|FP1_MYTGA Adhesive plaque matrix protein precursor (Foo...t protein 1) (MGFP1) (MGFP-1) dbj|BAA09851.1| adhesive plaque protein [Mytilus galloprovincialis] Q27409 6e-22 32% ...

  14. NCBI nr-aa BLAST: CBRC-MLUC-01-0191 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MLUC-01-0191 ref|NP_001013440.1| similar to CfOLF4 [Canis lupus familiaris] sp...|Q95157|OLF4_CANFA RecName: Full=Olfactory receptor-like protein OLF4 gb|AAB37242.1| CfOLF4 [Canis lupus familiaris] NP_001013440.1 2e-87 75% ...

  15. NCBI nr-aa BLAST: CBRC-AGAM-01-0071 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-01-0071 ref|NP_476722.1| shotgun CG3722-PA [Drosophila melanogaster] sp|Q...24298|CADE_DROME DE-cadherin precursor (Protein shotgun) gb|AAF46659.1| CG3722-PA [Drosophila melanogaster] NP_476722.1 0.0 40% ...

  16. NCBI nr-aa BLAST: CBRC-AGAM-01-0070 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-AGAM-01-0070 ref|NP_476722.1| shotgun CG3722-PA [Drosophila melanogaster] sp|Q...24298|CADE_DROME DE-cadherin precursor (Protein shotgun) gb|AAF46659.1| CG3722-PA [Drosophila melanogaster] NP_476722.1 0.0 42% ...

  17. NCBI nr-aa BLAST: CBRC-DNOV-01-0327 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-0327 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 0.0 79% ...

  18. NCBI nr-aa BLAST: CBRC-RMAC-05-0004 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RMAC-05-0004 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 0.0 84% ...

  19. NCBI nr-aa BLAST: CBRC-OLAT-05-0023 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OLAT-05-0023 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 1e-169 70% ...

  20. NCBI nr-aa BLAST: CBRC-FRUB-02-0554 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FRUB-02-0554 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 1e-124 64% ...

  1. NCBI nr-aa BLAST: CBRC-ACAR-01-0406 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ACAR-01-0406 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 1e-138 63% ...

  2. NCBI nr-aa BLAST: CBRC-HSAP-02-0034 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-HSAP-02-0034 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 0.0 78% ...

  3. NCBI nr-aa BLAST: CBRC-TGUT-06-0017 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TGUT-06-0017 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 1e-179 70% ...

  4. NCBI nr-aa BLAST: CBRC-PTRO-05-0012 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PTRO-05-0012 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 0.0 83% ...

  5. NCBI nr-aa BLAST: CBRC-CFAM-03-0024 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CFAM-03-0024 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 0.0 83% ...

  6. NCBI nr-aa BLAST: CBRC-PABE-05-0009 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PABE-05-0009 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 0.0 83% ...

  7. NCBI nr-aa BLAST: CBRC-OCUN-01-0165 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OCUN-01-0165 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 1e-156 79% ...

  8. NCBI nr-aa BLAST: CBRC-HSAP-01-0064 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-HSAP-01-0064 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 0.0 79% ...

  9. NCBI nr-aa BLAST: CBRC-ETEL-01-0092 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ETEL-01-0092 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 1e-129 74% ...

  10. NCBI nr-aa BLAST: CBRC-CJAC-01-0720 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CJAC-01-0720 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 0.0 82% ...

  11. NCBI nr-aa BLAST: CBRC-GACU-17-0020 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GACU-17-0020 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 1e-167 69% ...

  12. NCBI nr-aa BLAST: CBRC-OANA-01-1403 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OANA-01-1403 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 1e-104 53% ...

  13. NCBI nr-aa BLAST: CBRC-FCAT-01-1119 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-1119 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 0.0 85% ...

  14. NCBI nr-aa BLAST: CBRC-SARA-01-0200 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-SARA-01-0200 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 3e-47 59% ...

  15. NCBI nr-aa BLAST: CBRC-GGAL-04-0039 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GGAL-04-0039 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 0.0 70% ...

  16. NCBI nr-aa BLAST: CBRC-FRUB-02-0376 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FRUB-02-0376 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 1e-164 68% ...

  17. NCBI nr-aa BLAST: CBRC-PTRO-02-0027 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PTRO-02-0027 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 0.0 79% ...

  18. NCBI nr-aa BLAST: CBRC-MMUS-05-0015 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-05-0015 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 0.0 95% ...

  19. NCBI nr-aa BLAST: CBRC-RNOR-14-0117 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-14-0117 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 0.0 99% ...

  20. NCBI nr-aa BLAST: CBRC-SARA-01-0834 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-SARA-01-0834 ref|NP_036900.1| dopamine receptor D5 [Rattus norvegicus] sp|P25115|DRD5_RAT D(1B) dopamin...e receptor (D(5) dopamine receptor) gb|AAA41072.1| dopamine D1B receptor NP_036900.1 3e-47 59% ...