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Sample records for sp leucaena diversifolia

  1. Avaliação de genótipos de Leucaena spp. nas condições edafoclimáticas de São Carlos, SP: I. caracterização fenotípica e avaliação agronômica Evaluation of Leucaena spp. genotypes in the edaphic and climatic conditions of São Carlos, SP: I. phenotypical characterization and agronomical evaluation

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    A.C.P. de A. Primavesi

    1994-04-01

    Full Text Available Para avaliar genótipos promissores de Leucaena spp., foi instalado experimento em Latossolo Vermelho-Amarelo distrófico, na área da EMBRAPA-CPPSE em São Carlos, situada a 22°01'S e 47°53'W, com altitude de 856 m e média de precipitação anual de 1502 mm. Foram testados os seguintes genótipos: Leucaena leucocephala cv. Texas 1074 (T1, L.leucocephala 29 A9 (T2, L.leucocephala 11 x L.diversifolia 25 (T3, L.leucocephala 11 x L.leucocephala 26 (T4, L.leucocephala 24-19/2-39 X L.diversifolia 26 (T5 e L.leucocephala cv. Cunningham (testemunha. Através das avaliações efetuadas durante o período de estabelecimento (15 meses, verificou-se que: a as plantas de L.leucocephala 24-19/2-39 x L.diversifolia 26 se mostraram mais ramificadas e com maior produção de sementes; b as plantas de L.leucocephala 11 x L.diversifolia 26 apresentaram a maior produção de matéria seca consumível; c os materiais apresentaram brotação após o corte, semelhante ou inferior à testemunha.To test promising genotypes of Leucaena spp., selected in a breeding program, an experiment was conducted in a distrofic Red-Yellow Latossol, at the experimental station of EMBRAPA/CPPSE, São Carlos,SP, located at 22°01' and 47°53'W, altitude of 856 m and with a mean annual precipitation of 1502 mm. The following genotypes were tested: L.leucocephala cv. Texas 1074 (T1, L.leucocephala 29 A9 (T2, L.leucocephala 11 x L.diversifolia 25 (T3, L.leucocephala 11 x L.diversifolia 26 (T4, L.leucocephala 24-19/2-39 x L.diversifolia 26 (T5 and L.leucocephala cv. Cunningham (control. Evaluations were performed during the establishment period (fifteen months and it was concluded that: a the plants of L.leucocephala 24-19/2-39 x L.diversifolia 26, with early flowering, were the most branched and presented the greatest seed yield; b the plants of L.leucocephala 11 x L.diversifolia 26, showed the greatest edible dry matter yield; c the genotypes presented similar or worse growth, than

  2. Leucaena sp. recombinant cinnamyl alcohol dehydrogenase: purification and physicochemical characterization.

    Science.gov (United States)

    Patel, Parth; Gupta, Neha; Gaikwad, Sushama; Agrawal, Dinesh C; Khan, Bashir M

    2014-02-01

    Cinnamyl alcohol dehydrogenase is a broad substrate specificity enzyme catalyzing the final step in monolignol biosynthesis, leading to lignin formation in plants. Here, we report characterization of a recombinant CAD homologue (LlCAD2) isolated from Leucaena leucocephala. LlCAD2 is 80 kDa homo-dimer associated with non-covalent interactions, having substrate preference toward sinapaldehyde with Kcat/Km of 11.6×10(6) (M(-1) s(-1)), and a possible involvement of histidine at the active site. The enzyme remains stable up to 40 °C, with the deactivation rate constant (Kd(*)) and half-life (t1/2) of 0.002 and 5h, respectively. LlCAD2 showed optimal activity at pH 6.5 and 9 for reduction and oxidation reactions, respectively, and was stable between pH 7 and 9, with the deactivation rate constant (Kd(*)) and half-life (t1/2) of 7.5×10(-4) and 15 h, respectively. It is a Zn-metalloenzyme with 4 Zn(2+) per dimer, however, was inhibited in presence of externally supplemented Zn(2+) ions. The enzyme was resistant to osmolytes, reducing agents and non-ionic detergents. Copyright © 2013 Elsevier B.V. All rights reserved.

  3. Tithonia diversifolia (Hemsl. A. Gray Tithonia diversifolia (Hemsl. A. Gray

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    A Pérez

    2009-03-01

    Full Text Available El género Tithonia, con más de 10 especies, es originario de Centroamérica pero se encuentra ampliamente distribuido en el área tropical de diferentes continentes, lo que le confiere una gran plasticidad ecológica. Tithonia diversifolia es una planta herbácea o arbustiva robusta, conocida con diversos nombres comunes que identifican o manifiestan su amplitud de usos benéficos o características parecidas a otras plantas, como son árbol maravilla, falso girasol y árnica de la tierra, entre otros. El presente artículo trata diferentes aspectos de esta especie, tales como: la botánica, su origen y distribución, los factores agronómicos y la producción de biomasa, el contenido nutricional y los metabolitos secundarios, además de su utilización. Se incluyen incipientes resultados de la República de Cuba, así como algunas interrogantes acerca de los metabolitos secundarios, en las cuales se debe profundizar. T. diversifolia es capaz de adaptarse a las más diversas condiciones ecológicas; su valor nutricional y composición bromatológica, así como la poca exigencia a las labores fitotécnicas y los elevados rendimientos de biomasa (la cual puede ser consumida por las más diversas categorías de animales la convierten en un recurso fitogenético excepcional. Como fuente de alto valor proteico, se utiliza en pastoreo o como forraje para rumiantes y monogástricos; además, en la dieta alimentaria puede ofrecerse presecada o molida en forma de harina y pienso; asimismo, es posible su almacenamiento por períodos relativamente largos. Sin dudas, es una nueva opción de alimento que puede ser empleada para paliar la carencia alimentaria, sobre todo en los países del trópico con insuficientes insumos y recursos.The Tithonia genus, with more than 10 species, originated in Central America, but it is widely spread in the tropical area of different continents, which grants it great ecological plasticity. Tithonia diversifolia is a robust

  4. Effect of Tithonia diversifolia mulch on Atta cephalotes (Hymenoptera: Formicidae) nests.

    Science.gov (United States)

    Rodríguez, Jonathan; Montoya-Lerma, James; Calle, Zoraida

    2015-01-01

    Recent studies have shown an insecticidal effect of Tithonia diversifolia (Hemsl.) Gray (Asterales: Asteraceae) foliage on workers of Atta cephalotes L. and inhibitory effects of this plant on the growth of the symbiotic fungus Leucoagaricus gongylophorus (A. Müler) Singer. To evaluate the potential of T. diversifolia as a biological control treatment of this important pest, we assessed the effect of green manure (mulch) of this plant on natural nests of A. cephalotes, in Cali, Colombia. Three treatments were randomly assigned to 30 nests: 1) green mulch of T. diversifolia, 2) green mulch of Miconia sp., Ruiz & Pav. and 3) unmulched control. Every 2 wk for 6 mo, the surface of the nests was completely covered with leaves. Physical and chemical parameters of nest soil were assessed before the first and after the last application of the mulch. Ant foraging in T. diversifolia-treated nests decreased by 60% after the initial applications of the mulch, while nest surface area decreased by 40%. When the nests covered with T. diversifolia were opened, it was observed that the superficial fungus chambers had been relocated at a greater depth. In addition, microbial activity and soil pH increased by 84% and 12%, respectively, in nests covered with plant residues. In conclusion, the continued use of T. diversifolia mulch reduces foraging activity and negatively affects the internal conditions of the colonies, thereby inducing the ants to relocate the fungus chambers within the nests. © The Author 2015. Published by Oxford University Press on behalf of the Entomological Society of America.

  5. GROWTH AND NODULATION OF LEUCAENA AND PROSOPIS SEEDLINGS IN SOIL PLUS TANNERY SLUDGE

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    Ademir Sérgio Ferreira de Araújo

    2006-01-01

    Full Text Available The objective of this paper was to evaluate the growth and nodulation of Leucaena and Prosopis seedlings in soil plus tannery sludge. The study was conducted in a greenhouse, using bags containing soil plus tannery sludge. Seedlings of Leucaena and Prosopis inoculated with Bradyrhizobium sp were used. Results were evaluated 90 days after plant emergency. The application of tannery sludge, in the rate of 11.250 kg per hectare significantly increased plant height, stem diameter, and above ground dry mass ofLeucaena. Regarding Prosopis, there were no significant increases in these variables with tannery sludge application, except for aerial dry mass. No significant differences were seen between the treatments with sludge and inoculated with Bradyrhizobium sp. as to nodule number and dry mass. Tannery sludge evaluated in this work did not affect nodulation, besides favoring Leucaena and Prosopis seedling growth.

  6. Comportamiento fenológico de 23 accesiones de Leucaena spp Phenological performance of 23 Leucaena spp. accessions

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    Hilda B Wencomo

    2010-12-01

    Full Text Available Se evaluaron 23 accesiones de Leucaena spp. de una colección de 180, con el objetivo de caracterizar el comportamiento de sus patrones fenológicos en la etapa de establecimiento; las plantas se sembraron a una distancia de 6 x 3 m entre surcos y entre plantas. Se contó el número de pinnas por hoja y de pínulas por pinna; además se midió la longitud y el ancho de las pínulas, la cantidad de legumbres por cabezuela, la longitud y el ancho de las legumbres. Se tuvo en cuenta la forma de las pinnas, el tipo y la posición de las glándulas y el color de las flores. La aparición de flores y frutos fue diferente entre las especies de este género, y se presentó sólo de forma anual en algunas de ellas. Las accesiones de cada especie tuvieron un comportamiento similar, tanto en lluvia como en seca, excepto L. diversifolia CIAT-17503, L. macrophylla CIAT-17233 y L. esculenta CIAT-17229 (las cuales se mantuvieron en fase vegetativa. Asimismo se pudo apreciar que las flores individuales de las especies y accesiones de Leucaena generalmente son pequeñas. Se concluye que existen diferencias entre las especies y accesiones evaluadas en esta fase; al igual que en el comportamiento de sus patrones de floración y fructificación.Twenty three Leucaena spp. accessions of a collection of 180 were evaluated, in order to characterize the performance of their phenological patterns in the establishment stage; the plants were sown at a distance of 6 x 3 m between rows and between plants. The number of pinnae per leaf and of pinnule per pinna was counted; in addition, the length and width of the pinnule were measured, and the quantity of legumes per capitulum, the length and width of the pods were determined. The form of the pinnae, the type and position of the glands and the color of he flowers were taken into consideration. The emergence of flowers and fruits was different among the species of this genus, and it occurred only annually in some of them. The

  7. Leucaena leucocephala IN RUMINANT NUTRITION

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    M. Barros-Rodríguez

    2014-08-01

    Full Text Available It is a common situation in extensive ruminant production systems in tropical countries to have low production indicators due to nutrient deficiencies in the diet. An economic alternative to increase animal production is the incorporation of legumes (fodder and fruits in the diet. This review, presents an analysis of the positive and negative effects of Leucaena leucocephala consumption by ruminants, with particular emphasis on the secondary compound mimosine. Leucaena due to its high nutrient content, rumen by-pass protein supply and its possible effect on the reduction of greenhouse gas (attributed to tannins has become one of the legumes most commonly used in ruminant feeding practices. However, in countries where leucaena has been introduced, its use is still limited to levels below 30% inclusion in the diet, due to the secondary compound mimosine and its isomers (3,4 and 2,3 DHP, which can induce toxicity, even when animals are inoculated with rumen fluid containing the bacteria Synergistes jonesii reported as responsible for degrading these compounds in the rumen. In the Yucatan Peninsula, Mexico, ruminants consuming leucaena can tolerate more than 50% inclusion in the diet, without having a negative impact on production, attributed intake to mimosine and its isomers. We conclude that in animals not adapted, the intake would be limited to low inclusion levels (less than 30% inclusion in the diet, mainly because of mimosine and its derivatives. The decrease in intake or diet digestibility seem to better explain the reduction in methane production, however, in vivo studies are required to clearly establish the mechanism of action. It has been reported the presence of different bacteria to S. jonessi that would have the ability to degrade mimosine and its derivatives, however, the activity of these bacteria and its effectiveness must be confirmed in vivo.

  8. Nutrient Dynamics and Litter Decomposition in Leucaena ...

    African Journals Online (AJOL)

    Nutrient contents and rate of litter decomposition were investigated in Leucaena leucocephala plantation in the University of Agriculture, Abeokuta, Ogun State, Nigeria. Litter bag technique was used to study the pattern and rate of litter decomposition and nutrient release of Leucaena leucocephala. Fifty grams of oven-dried ...

  9. Capacidad de recuperación de 23 accesiones de Leucaena spp. después de la poda Recovery capacity of 23 Leucaena spp. accessions after pruning

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    Hilda B Wencomo

    2011-03-01

    Full Text Available Se evaluaron 23 accesiones de Leucaena spp. durante dos años, con el objetivo de conocer la variabilidad existente en la población en cuanto a la capacidad de recuperación después de la poda. Cuando las plantas sobrepasaban los 2 m, se realizó la poda de uniformidad a un 1 m de altura sobre el nivel del suelo. Las mediciones fueron: el grosor del tallo, el número de rebrotes y su longitud con una frecuencia semanal, a partir de lo cual se calculó la velocidad de crecimiento de cada accesión; además se determinó el rendimiento. El análisis de componentes principales, el de conglomerados y el de regresión lineal simple se realizaron a través del programa estadístico SPSS versión 11.5 para Microsoft Windows®. Se observó variabilidad en los indicadores evaluados (86,11% acumulado en la componente y todos contribuyeron a su explicación. A su vez, el análisis de conglomerados permitió la formación de tres grupos. Se concluye que todas las accesiones presentaron capacidad de recuperación después de la poda, con diferencias entre ellas. Las accesiones Leucaena leucocephala cv. Cunningham, cv. Perú, CIAT-9119, CIAT-9438, CIAT-751, CIAT-7988, CIAT-7384, CIAT-7929, CIAT-17480, cv. Ipil-Ipil y cv. CNIA-250; L. lanceolata CIAT-17255 y CIAT-17501 y L. diversifolia CIAT-17270 fueron las de mayor capacidad de recuperación. Por ello se recomienda, en futuras investigaciones relacionadas con la caracterización, evaluación y selección de estas especies, realizar estudios que incluyan su persistencia en el tiempo bajo condiciones de poda, así como su empleo en el fomento de nuevos sistemas silvopastoriles.Twenty three Leucaena spp. accessions were evaluated for two years, in order to know the variability existing in the population regarding the recovery capacity after pruning. When the plants were higher than 2 m, the uniformity pruning was made at 1 m above the soil level. The measurements were: stem diameter, number of regrowths and

  10. Dormancia y tratamientos pregerminativos en las semillas de Leucaena spp. cosechadas en suelo ácido Dormancy and pregerminative treatments in Leucaena spp. seeds harvested on acid soil

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    Yolanda González

    2009-12-01

    Full Text Available Se desarrolló un experimento con semillas de accesiones de Leucaena spp. cosechadas en suelo ácido y almacenadas bajo condiciones ambientales, para determinar el período e intensidad de la dormancia y su ruptura. Para ello se empleó un diseño de clasificación simple y cuatro réplicas, con los siguientes tratamientos: 1 semilla sin tratar (control; 2 corte de cubierta; y 3 agua a 80°C durante 2 minutos. En cada una de las accesiones hubo diferencias significativas entre los tratamientos aplicados y los mayores valores de germinación se encontraron con el corte de cubierta. Todas las accesiones mostraron estado dormático (entre 10 y 98%, el cual se apreció en la germinación del control y en la viabilidad con el corte de cubierta. Los mayores valores de dormancia en las semillas cosechadas en el 2006 se detectaron en L. macrophylla CIAT 17233, L. macrophylla CIAT 17231, L. diversifolia CIAT 17270 y L. leucocephala IH-1140, cuya dureza fue de 98,0; 97,0; 80,0 y 74,7%, respectivamente; en las cosechadas en el 2003 y 2004, los porcentajes de dureza fueron de 89,0; 69,0 y 60,0% para L. lanceolata CIAT 17252, L. macrophylla CIAT 17238 y L. leucocephala cv. K-67, respectivamente. Se concluye que las semillas de las accesiones presentaron dormancia poscosecha, que varió entre 10 y 98%. Además la escarificación térmica provocó su ruptura, por lo que se recomienda aplicar este procedimiento antes de la siembra a las semillas de Leucaena spp. que se cosechen en suelo ácido de Cascajal.A trial was conducted with seeds of Leucaena spp accessions harvested on acid soil and stored under ambient conditions, in order to determine the period and intensity of dormancy and its breaking. For that purpose a simple classification design and four replications were used, with the following treatments: 1 untreated seed (control; 2 coat cut; and 3 water at 80ºC for 2 minutes. In each one of the accessions there were significant differences among the

  11. Studies on the variations in the chemical composition of leucaena ...

    African Journals Online (AJOL)

    The fermentation quality and nutritive value of leucaena ensiled either as whole forage or separate stem and leaf fractions were investigated. About 10-month-old leucaena (Leucaena leucocephala (Lam.) De Wit.) grown in Okinawa Island, Japan, was used.Samples were chopped to about 4 cm lengths and stuffed into ...

  12. Phytotoxic property of the invasive plant Tithonia diversifolia and a phytotoxic substance.

    Science.gov (United States)

    Suzuki, Masahiko; Iwasaki, Arihiro; Suenaga, Kiyotake; Kato-Noguchi, Hisashi

    2017-06-01

    Tithonia diversifolia (Hermsl.) A. Gray is a perennial invasive plant and spreads quickly in the invasive areas. The extracts of T. diversifolia were found to be toxic to several crop plant species such as rice, maize, sorghum, lettuce and cowpea, and several putative allelopathic substances were identified. However, there is limited information available for the effects of T. diversifolia on wild plants including weed plant species. We investigated the allelopathic potential of T. diversifolia extracts on weed plants, and searched for phytotoxic substances with allelopathic activity. An aqueous methanol extract of T. diversifolia leaves inhibited the growth of weed plants, Lolium multiflorum Lam., Phleum pretense L., Echinochloa crus-galli (L.) Beauv. The extract was then purified by several chromatographic runs and a phytotoxic substance with allelopathic activity was isolated and identified by spectral analysis as tagitinin C. The substance inhibited the growth of Lolium multiflorum, Phleum pratense and Echinochloa crus-galli at concentrations greater than 0.1 - 0.3 mM. The present results suggest that T. diversifolia may possess allelopathic potential on weed plants and tagitinin C may be responsible for the allelopathic effects of T. diversifolia. The allelopathic potential of T. diversifolia may contribute to its invasive characteristics.

  13. Research Note: Predicting Leucaena leucocephala biomass from ...

    African Journals Online (AJOL)

    The amount of leaf and stem material removed by browsers can be precisely (r2 >0.90) and accurately (within 11% of the mean) predicted from DPB, thus providing a simple means of estimating browse offtake from leucaena trees. Keywords: Browse, firewood, regression, tree height, twig diameter. African Journal of Range ...

  14. Antioxidant and tyrosinase inhibitor from Leucaena leucocephala

    African Journals Online (AJOL)

    gabriel

    2012-09-25

    Sep 25, 2012 ... and Chung-Yi Chen ... both synthetic (Lee et al., 2009; Liu et al., 2009) and ... Leucaena leucocephala is a leguminous plant which is ... et al., 2000). ..... Li et al. 14185. Nakatani Y, Ourisson G, Beck JP (1981). Chemistry and ...

  15. Avaliação de genótipos de Leucaena spp. nas condições edafoclimáticas de São Carlos,SP: II. determinações bromatológicas no período de estabelecimento Evaluation of Leucaena spp. genotypes in the edaphic and climatic conditions of São Carlos, SP: II. bromatological determinations at the establishment period

    Directory of Open Access Journals (Sweden)

    A.C.P. de A. Primavesi

    1994-04-01

    Full Text Available Em experimento conduzido em Latossolo Vermelho-Amarelo distrófico, em área da EMBRAPA - CPPSE em São Carlos, situada a 22°01'S e 47°53'W, com altitude de 856 m e média de precipitação anual de 1502 mm, procedeu-se a determinação da composição bromatológicade folhas, hastes com diâmetro menor que 6 mm e vagens, de genótipos de leucena. Os genótipos avaliados, foram: L.leucocephala cv. Texas 1074 (TI, L.leucocephala 29 A9 (T2, L.leucocephala 11 x L.dlversifolia 25 (T3, L.leucocephala 11 x L.diversifolia 26 (T4, L.leucocephala 24-19/2-39 x L.diverstfolia 26 (T5 e L.leucocephala c v. Cunningham (testemunha. Verificou-se que: os genótipos avahados não apresentaram diferenças nas determinações bromatológicas, realizadas nas folhas e talos finos; o genótipo T3 registrou o maior teor de proteína bruta (28,06%, de fósforo (0,29% e a maior relação PB/FDN e o menor teor de FDN para vagens; os genótipos apresentaram os seguintes teores médios, em porcentagem, para a composição bromatológicadas folhas, vagens e talos finos, respectivamente: Proteína bruta (18,57; 21,68; 6,41; Fibra detergente neutro (29,09; 41,58; 71,01; Fósforo (0,12; 0,22; 0,06; Cálcio (1,39; 0,36; 0,49; Magnesio (0,51; 0,28; 0,24; Tanino (1,32; 1,15; 0,28 e Digestibilidade "in vitro" (58,39; 61,22; 33,61; os teores de proteína e fósforo apresentaram a seguinte ordem decrescente nas partes das plantas: vagens > folhas > talos finos; os teores de cálcio: folhas > talos finos > vagens e de magnésio: folhas > vagens > talos finos.In a trial conducted on a distrofic Red-Yellow Latossol, at EMBRAPA-CPPSE, São Carlos, located at 22°01'S and 47'53'W, altitude of 856 m and with a mean annual rainfall of 1502 mm, the bromatological composition of leaves, stems smaller than 6 mm diameter and pods of leucena genotypes was determined. The genotypes evaluated were: L.leucocephala cv. Texas 1074 (T1, L.leucocephala 29 A9 (T2, L.leucocephala 11 x L.dlversifolia 25

  16. Variación interespecífica de la calidad nutricional de diecisiete accesiones de Leucaena

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    D. E. García

    2008-01-01

    Full Text Available Se realizó un experimento para evaluar, mediante el análisis de componentes principales (ACP, la variabilidad de la composición nutricional del follaje de diecisiete accesiones del género Leucaena (8 L. leucocephala, 5 L. lanceolata, 2 L. diversifolia, 1 L. glauca y 1 L. esculenta establecidas en Matanzas, Cuba, en condiciones tropicales y suelo Ferralítico Rojo Lixiviado. Se determinó el perfil bromatológico, los niveles de metabolitos secundarios y la degradabilidad ruminal. Mediante el ACP se formaron tres componentes y se extrajo un porcentaje de varianza intermedio (64.83. Los indicadores que explicaron mejor las variaciones entre las accesiones fueron la fracción proteica (PC y PV, la lignina, los compuestos polifenólicos (FT, TPP, TC, los fitatos y la degradabilidad ruminal (DMS, DPC, DFDN. Sin embargo, los niveles de MS, fracción fibrosa (FDN, FDA, FC, celulosa, mimosina y minerales presentaron variaciones menos importantes. Se identificaron tres grupos variabilicon características nutricionales diferenciadas. Aunque todas las accesiones presentaron buena composición proximal, las accesiones de L. diversifolia y L. esculenta presentaron mayor concentración de metabolitos secundarios y menor degradabilidad ruminal. En todos los casos la degradación de la MS, PC y FDN se vio afectada por los contenidos de polifenoles y fitatos. Las accesiones de L. leucocephala (LPIII-187, IH- 164, IH-449, IH-1069, IH-1140, Ecotec, IRI- 3164, IRI-3219, L. lanceolata (CIAT-17223, CIAT-17501, CIAT-17252, CIAT-17255, CIAT-17256 y L. glauca presentaron los mejores resultados

  17. Evaluating the performance of Leucaena accessions for agroforestry ...

    African Journals Online (AJOL)

    The performance of Leucaena accessions on acid soils in eastern coast Tanzania for agroforestry was evaluated for three years. Plant tree parameters assessed were fodder yield and plant stem height, stem girth, the number of total branches and poles production. Also, Leucaena psyllid, a common pest threatening the ...

  18. Effects of Tithonia diversifolia, farmyard manure and urea, and phosphate fertiliser application methods on maize yields in western Kenya

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    Peter Asbon Opala

    2015-05-01

    Full Text Available Maize production in western Kenya is often limited by deficiencies of nitrogen and phosphorus. We assessed the effectiveness of Tithonia diversifolia green manure (tithonia, farmyard manure (FYM and urea as sources of nitrogen (N for maize when inorganic phosphorus (P fertiliser was either broadcast (BR or spot-placed in the planting hole (SP for two consecutive seasons; October to December of 1998 and April to August of 1999 at two sites; Nyabeda and Khwisero in western Kenya. A randomised complete block design with four replications was used. Maize yields were higher at Nyabeda and responded to P application better than at Khwisero. At the same N rate, tithonia and FYM were as effective as urea in increasing maize yields at both sites. There were no significant differences in maize yields when phosphate fertiliser was either BR or SP regardless of the N source used in the first season. However, in the second season, the residual yields for the BR treatments were consistently higher than those of the SP. Our results suggest that tithonia and FYM can substitute for urea as N sources and that fertiliser P should be broadcast and incorporated together with the organic materials at the time of planting to save on labour costs.

  19. Leucaena 2: the tree that defies the woodcutter

    Energy Technology Data Exchange (ETDEWEB)

    Benge, M D

    1981-07-01

    This article emphasises Leucaena's ability to obtain nutrients from soil strata that are not accessible to most other plants by means of its deep-growing root system. Surrounding the roots of Leucaena are masses of mychorrhizae that can metabolise unavailable phosphorous and other minerals which are then slowly released to the plant. In addition, symbiotic nitrogen-fixing bacteria fix nitrogen from the air. The plant then transfers minerals and nitrogen to its leaves. In one system of upland farming, Leucaena is intercropped with root crops and is cut and applied to the intercropped annual crops as an organic mulch, providing fertilizer that the marginal farmer could not otherwise afford.

  20. NUTRIENTS DIGESTIBILITY IN Tithonia diversifolia FOLIAGE IN FATTENING RABBITS

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    Duilio Nieves

    2010-10-01

    Full Text Available An experiment was carried out to determine the nutrients digestibility in Tithonia diversifolia foliage in fattening rabbits, 30 animals (1.450 g ± 93.77 initial body weight were distributed according to a completely randomized experimental design in three treatments and ten replicates. The mash diets including 0, 9 and 18 % of tithonia foliage. The dry matter (DMD, organic matter (OMD, crude protein (CPD, neutral detergent fiber (FDND, hemicellulose (HEMD and energy digestibilities (DE were determined using the acid insoluble ash method. The nutrient digestibility in foliage was estimated by the replacing test ingredient method. The DMD, OMD, PCD and HEMD (51.12, 53.45 and 51.25; 51.99, 54.87 and 52.60; 68.57, 60.11 and 64.08, and 44.20, 45.37 and 47.24 % for the three foliage inclusion level, respectively were similar (P>0.05 among diets. The foliage MSD, OMD, PCD, and ED DHEM was 53.80, 55.19, 59.17, 50.00 and 39.18%, while the protein and energy digestible content in tithonia foliage was 109.60 g/kg and 2139.45 kcal/kg. It was concluded that the tithonia foliage has high content of nutrients

  1. evaluating the performance of leucaena accessions for agroforestry

    African Journals Online (AJOL)

    Mgina

    The performance of Leucaena accessions on acid soils in eastern coast Tanzania for ... Plant tree parameters assessed were fodder yield and plant stem ... adapted and potential in improving soil fertility and are recommended for agroforestry.

  2. SUPPLY OF Tithonia diversifolia SILAGE ALONE OR MIXED WITH CASSAVA BRAN IN BROILERS DIET

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    Jaime Andrés Betancourt

    2017-08-01

    Full Text Available Tithonia diversifolia and cassava bran are two food resources that noted for their protein and energy levels, respectively. The aim of this study was to evaluate the response performance of broilers fed diets containing T. diversifolia silage alone or mixed with cassava bran. We used 150 females chickens, which were housed in 15 pens and each pen was assigned to the treatments according to a randomized experimental design. The treatments consisted of three diets that were supplied to birds between 35 and 56 d of age: a control where the birds were fed only concentrate and two other treatments in which the birds were supplied concentrate of control treatment and additionally offered T. diversifolia silage either alone or mixed with cassava bran. Silage supply did not affect live weight at 56 d of age, but resulted in a reduction in concentrate consumption (P<0.05 and the carcass weight (P<0.05. It was concluded that T. diversifolia silage can be used to decrease the consumption of concentrate, but is necessary to carry out a proper habituation to avoid decrease in dry matter intake.

  3. Nutrition of Tithonia diversifolia and attributes of the soil fertilized with biofertilizer in irrigated system

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    Matheus M. Reis

    Full Text Available ABSTRACT The fertilization with biofertilizer associated with the use of irrigation favors nutrient uptake by plants and soil chemical properties; however, these effects are little studied in Tithonia diversifolia in semiarid regions. This study evaluated the effect of doses of bovine biofertilizer and irrigation on accumulation of nutrients in the leaves of Tithonia diversifolia plants and on soil chemical attributes. The study was carried out from December 3, 2014 to November 28, 2015, and arranged in a 2 x 5 factorial scheme, consisting of five doses of bovine biofertilizer (0, 40, 80, 120 and 160 m3 ha-1, combined with and without irrigation. The experiment was set in a randomized block design, using three replicates. Irrigation promoted increased accumulation of N, P, K, Ca, Mg, S, Zn, Fe, Mn, Cu and B in leaves of Tithonia diversifolia in the first cutting. However, the high bicarbonate concentration in the irrigation water and the occurrence of rainfall during the second crop increased the accumulation of Cu in the leaves of Tithonia diversifolia under rainfed condition, compared with irrigated plants. The increase in biofertilizer doses contributed to the increment of base saturation and the contents of organic matter, P and K in soil.

  4. Systemic effects of Leucaena leucocephala ingestion on ringtailed lemurs (Lemur catta) at Berenty Reserve, Madagascar.

    Science.gov (United States)

    Crawford, Graham; Puschner, Birgit; Affolter, Verena; Stalis, Ilse; Davidson, Autumn; Baker, Tomas; Tahara, John; Jolly, Alison; Ostapak, Susan

    2015-06-01

    Leucaena (Leucaena leucocephala) is a leguminous tree that is nutritious forage for domestic livestock when ingested in limited amounts. Unfortunately, leucaena contains mimosine, a plant amino acid, that can be toxic when ingested at higher concentrations. Reported toxic effects include alopecia (fur loss), poor body condition, infertility, low birth weight, thyroid gland dysfunction, and organ toxicity. Originally native to Mexico and Central America, leucaena has been introduced throughout the tropics, including Berenty Reserve, Madagascar where it was planted as supplemental browse for livestock. In Berenty, a seasonal syndrome of alopecia in ringtailed lemurs (Lemur catta) is associated with eating leucaena. Although much is known about the toxic effects of leucaena and mimosine on domestic animals and humans, the systemic effects on wildlife had not been studied. In a comparison of lemurs that include leucaena in their diet and those that do not, we found that animals that ingest leucaena absorb mimosine but that ingestion does not affect body condition, cause kidney or liver toxicity, or affect the intestinal tract. Alopecia is due to mimosine's interference of the hair follicle cycle. Leucaena ingestion is associated with higher serum albumin, α-tocopherol, and thyroxine concentrations, suggesting that leucaena may provide some nutritional benefit and that lemurs can detoxify and convert mimosine to a thyroid stimulating metabolite. The primary conservation consequence of leucaena ingestion at Berenty may be increased infant mortality due to the infants' inability cling to their alopecic mothers. The widespread introduction of leucaena throughout the tropics and its rapid spread in secondary forest conditions mean that many other leaf-eating mammals may be including this tree in their diet. Thus, exposure to leucaena should be considered when wildlife health is being evaluated, and the potential effects on wildlife health should be considered when

  5. Response of rabbits to varying levels of cassava and Leucaena ...

    African Journals Online (AJOL)

    Response of rabbits to varying levels of cassava and Leucaena leucocephala leaf meal diets. ... Bulletin of Animal Health and Production in Africa ... An experiment was carried out to determine the performance, haematology, carcass characteristics and sensory evaluation of meat from rabbits (n = 30) fed varying levels of ...

  6. EFFECT OF Dactyladenia barteri (Hook. f. ex Oliv.), Leucaena ...

    African Journals Online (AJOL)

    A two year field study was conducted to investigate the effects of prunings of Dactyladenia barteri (Hook. f. ex Oliv.), Leucaena leucocephala (Lam.) de Wit, Gliricidia sepium (Jacq.), and Senna siamea (Lam.) on the activity of earthworms and soil and worm cast properties. Earthworm activity was measured by quantifying ...

  7. Soil Solution Phosphorus Status and Mycorrhizal Dependency in Leucaena leucocephala†

    Science.gov (United States)

    Habte, Mitiku; Manjunath, Aswathanarayan

    1987-01-01

    A phosphorus sorption isotherm was used to establish concentrations of P in a soil solution ranging from 0.002 to 0.807 μg/ml. The influence of P concentration on the symbiotic interaction between the tropical tree legume Leucaena leucocephala and the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus fasciculatum was evaluated in pot experiments. The level of mycorrhizal infection in Leucaena roots increased as the concentration of P was raised from 0.002 to 0.153 μg/ml. Higher levels of P depressed mycorrhizal infection, but the level of infection never declined below 50%. Periodic monitoring of P contents of Leucaena subleaflets indicated that significant mycorrhizal activity was detected as early as 17 days after planting, with the activity peaking 12 to 16 days thereafter. The highest level of mycorrhizal activity was associated with a soil solution P level of 0.021 μg/ml. Even though the mycorrhizal inoculation effect diminished as the concentration of P in the soil solution was increased, mycorrhizal inoculation significantly increased P uptake and dry-matter yield of Leucaena at all levels of soil solution P examined. The concentration of P required by nonmycorrhizal L. leucocephala for maximum yield was 27 to 38 times higher than that required by mycorrhizal L. leucocephala. The results illustrate the very high dependence of L. leucocephala on VAM fungi and the significance of optimizing soil solution phosphorus for enhancing the benefits of the VAM symbiosis. PMID:16347323

  8. The flavonoid constituents of Leucaena Leucocephala growning in ...

    African Journals Online (AJOL)

    ... Leucaena leucocephala were identified as Caffeic acid, Isorhamnetin, Chrysoeriol, Isorhamnetin 3-O-galactoside, Kaempferol-3-O-rubinoside, Quercetin-3-O-rhamnoside and Luteolin-7-glucoside. Chemical structures of the isolated compounds were identified by TLC, PC and spectral techniques (UV, 1H -NMR and MS).

  9. Soil Solution Phosphorus Status and Mycorrhizal Dependency in Leucaena leucocephala.

    Science.gov (United States)

    Habte, M; Manjunath, A

    1987-04-01

    A phosphorus sorption isotherm was used to establish concentrations of P in a soil solution ranging from 0.002 to 0.807 mug/ml. The influence of P concentration on the symbiotic interaction between the tropical tree legume Leucaena leucocephala and the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus fasciculatum was evaluated in pot experiments. The level of mycorrhizal infection in Leucaena roots increased as the concentration of P was raised from 0.002 to 0.153 mug/ml. Higher levels of P depressed mycorrhizal infection, but the level of infection never declined below 50%. Periodic monitoring of P contents of Leucaena subleaflets indicated that significant mycorrhizal activity was detected as early as 17 days after planting, with the activity peaking 12 to 16 days thereafter. The highest level of mycorrhizal activity was associated with a soil solution P level of 0.021 mug/ml. Even though the mycorrhizal inoculation effect diminished as the concentration of P in the soil solution was increased, mycorrhizal inoculation significantly increased P uptake and dry-matter yield of Leucaena at all levels of soil solution P examined. The concentration of P required by nonmycorrhizal L. leucocephala for maximum yield was 27 to 38 times higher than that required by mycorrhizal L. leucocephala. The results illustrate the very high dependence of L. leucocephala on VAM fungi and the significance of optimizing soil solution phosphorus for enhancing the benefits of the VAM symbiosis.

  10. Reproductive indices of Merino rams fed sun-cured Leucaena ...

    African Journals Online (AJOL)

    Reproductive indices of Merino rams fed sun-cured Leucaena leucocephala forage. I.V. Nsahlai, B.K. Byebwa, M.L.K. Bonsi. Abstract. (South African J of Animal Science, 2000, 30, Supplement 1: 111-112). Full Text: EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT.

  11. Regulating mineralization rates of Tithonia diversifolia and Lantana camara prunings to improve phosphorus availability in calcareous soils

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    Y. Nuraini

    2014-01-01

    Full Text Available The effect of mixing of Tithonia diversifolia and Lantana camara prunings to improve synchronization between P released from the prunings with crop demand for P was studied in a laboratory and in a glasshouse. Tithonia diversifolia prunings (Td, Lantana camara prunings (Lc, and farmyard manure (Pk were thoroughly mixed with the proportion (% of dry weight of; 25Td +75 Lc ; 50Td +50 Lc ; 75Td +25 Lc ; 90Lc +10 Pk ; 45Td +45 +10 Lc Pk ; 100Td and 100Lc, and then mixed with 100 g of air-dried soil with a rate equivalent to 100 kg P / ha. Results of the study showed that the pruning mixtures decomposed and mineralized faster than that of Lantana camara pruning only, but slower than that of Tithonia diversifolia pruning only. The amount of P released from the pruning mixtures increased with increasing proportion of Tithonia diversifolia pruning in the mixtures. Increasing proportion of Tithonia diversifolia pruning in the mixture applied to the soil increased the amount of P taken up by maize.

  12. POTENCIAL FORRAJERO DE Tithonia diversifolia Hemsl. A Gray EN LA PRODUCCIÓN DE VACAS LECHERAS

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    Luis Alberto Gallego-Castro

    2014-01-01

    Full Text Available Potencial forrajero de Tithonia diversifolia Hemsl. A Gray en la producción de vacas lecheras. El objetivo de este trabajo fue analizar el uso potencial de la Tithonia diversifolia (Hemsl. A. Gray (botón de oro en la alimentación de vacas lecheras en el trópico alto colombiano. Se eligieron términos clave para la búsqueda de información y a partir de ellos se abordaron y analizaron diferentes publicaciones, permitiendo un acercamiento a la problemática propuesta. En estos sistemas de producción típicos del trópico alto en Colombia, el kikuyo (Pennisetum clandestinum contribuye con el mayor aporte en la ración del ganado y debido al alto N, la baja fibra y materia seca, lleva con frecuencia a balances energéticos negativos en las vacas más productivas, por lo que en muchos casos se sostiene la producción con alimento comercial, compuesto principalmente por cereales y con altos niveles de proteína. Las necesidades nutricionales de este tipo de sistemas productivos están orientados a encontrar estrategias que permitan mejorar la oferta forrajera, en términos de variedad y calidad, disminuir la dependencia de alimentos comerciales o al menos facilitar la inclusión de otros que mejoren el desempeño animal. A partir de este análisis, se evidencia el potencial de T. diversifolia en la alimentación de vacas lecheras de alta producción; esta forrajera arbustiva, por su contenido de proteína, carbohidratos solubles y taninos, puede tener un impacto positivo sobre los sistemas de ganadería lechera intensiva y puede incorporarse a suplementos alimenticios.

  13. Effect of cutting height and frequency on Leucaena leucocephala ...

    African Journals Online (AJOL)

    Leucaena leucocephala is a fast-growing tree that can provide both high quality forage and firewood. The objective of this trial was to determine the optimum height and frequency of cutting for both wood and forage production. Cutting heights at 0.3m, 0.6m and 1.0 m were superimposed on 3-month and 6-month cutting ...

  14. STUDIES ON SOME PHYSICOCHEMICAL PROPERTIES OF LEUCAENA LEUCOCEPHALA BARK GUM

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    Vijetha Pendyala

    2010-06-01

    Full Text Available Gum exudates from Leucaena Leucocephala (Family: Fabaceae plants grown all over India were investigated for its physicochemical properties such as pH, swelling capacity and viscosities at different temperatures using standard methods. Leucaena Leucocephala bark gum appeared to be colorless to reddish brown translucent tears. 5 % w/v mucilage has pH of 7.5 at 28°C. The gum is slightly soluble in water and practically insoluble in ethanol, acetone and chloroform. It swells to about 5 times its original weight in water. A 5 %w/v mucilage concentration gave a viscosity value which was unaffected at temperature ranges (28-40°C. At concentrations of 2 and 5 %w/v, the gum exhibited pseudo plastic flow pattern while at 10 %w/v concentration the flow behaviour was thixotropic. The results indicate that the swelling ability of Leucaena Leucocephala (LL bark gum may provide potentials for its use as a disintegrant in tablet formulation, as a hydro gel in modified release dosage forms and the rheological flow properties may also provide potentials for its use as suspending and emulsifying agents owing to its pseudo plastic and thixotropic flow patterns.

  15. Effects of Aqueous Shoot Extract of Tithonia diversifolia on the Growth of Seedlings of Monodora tenuifolia (Benth.), Dialium guineense (Willd.) and Hildegardia barteri (Mast.) Kosterm

    OpenAIRE

    SAMSON OLAJIDE OKE; Abisola Victoria AWOWOYIN; Shaid Remi OSENI; Emmanuel Lekan ADEDIWURA

    2011-01-01

    The allelopathic effects of fresh shoot aqueous extract of Tithonia diversifolia (Hemsl.) A.Gray, an invasive species introduced into Africa from North and Central America, on the growth of seedlings of three woody species: Monodora tenuifolia, Dialium guineense and Hildegardia barteri were investigated. The fresh shoot aqueous extract of Tithonia diversifolia was applied to the established seedlings of the three woody species over a period of ten weeks. The fresh shoot aqueous extract of Tit...

  16. Characterization of Leucaena (Leucaena leucephala) oil by direct analysis in real time (DART) ion source and gas chromatography

    International Nuclear Information System (INIS)

    Alam, M.; Alandis, N.M.; Sharmin, E.; Ahmad, N.; Alrayes, B.F.; Ali, D.

    2017-01-01

    For the first time, we report the characterization of triacylglycerols and fatty acids in Leucaena (Leucaena leucephala) oil [LUCO], an unexplored nontraditional non-medicinal plant belonging to the family Fabaceae. LUCO was converted to fatty acid methyl esters (FAMEs). We analyzed the triacylglycerols (TAGs) of pure LUCO and their FAMEs by time-of-flight mass spectrometry (TOF-MS) followed by multivariate analysis for discrimination among the FAMEs. Our investigations for the analysis of LUCO samples represent noble features of glycerides. A new type of ion source, coupled with high-resolution TOF-MS was applied for the comprehensive analysis of triacylglycerols. The composition of fatty acid based LUCO oil was studied using Gas Chromatography (GC-FID). The major fatty acid components of LUCO oil are linoleic acid (52.08%) oleic acid (21.26%), palmitic acid (7.91%) and stearic acid (6.01%). A metal analysis in LUCO was done by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The structural elucidation and thermal stability of LUCO were studied by FT-IR, 1H NMR, 13C NMR spectroscopic techniques and TGA-DSC, respectively. We also measured the cytotoxicity of LUCO [es

  17. Novel anthraquinone derivatives produced by Phoma sorghina, an endophyte found in association with the medicinal plant Tithonia diversifolia (Asteraceae)

    OpenAIRE

    Borges, Warley de Souza; Pupo, Mônica Tallarico

    2006-01-01

    Three known anthraquinones (1,7-dihydroxy-3-methyl-9,10-anthraquinone, 1,6-dihydroxy-3-methyl-9,10-anthraquinone and 1-hydroxy-3-methyl-9,10-anthraquinone), one new anthraquinone (1,7-dihydroxy-3-hydroxymethyl-9,10-anthraquinone), and two new hexahydroanthraquinone derivatives, dendryols E and F, were isolated from the culture of the endophytic fungus Phoma sorghina, found in association with Tithonia diversifolia (Asteraceae). Their structures were identified on the basis of spectroscopic da...

  18. Anti-nitric oxide production, anti-proliferation and antioxidant effects of the aqueous extract from Tithonia diversifolia

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    Poonsit Hiransai

    2016-11-01

    Conclusions: Our study demonstrated the immunomodulation caused by the aqueous leaf extract of T. diversifolia, resulting from the inhibition of phytohemagglutinin-M-induced PBMCs proliferation and LPS-induced nitric oxide production in RAW264.7 macrophages. Although the anti-oxidative activity was presented in the chemical-based anti-oxidant assay, the extract cannot protect cell death from stress conditions.

  19. Repeated-dose toxicological studies of Tithonia diversifolia (Hemsl.) A. gray and identification of the toxic compounds.

    Science.gov (United States)

    Passoni, Flávia Donaire; Oliveira, Rejane Barbosa; Chagas-Paula, Daniela Aparecida; Gobbo-Neto, Leonardo; Da Costa, Fernando Batista

    2013-05-20

    Tithonia diversifolia (Hemsl.) A. Gray has been commonly used in folk medicine to treat abscesses, microbiological infections, snake bites, malaria and diabetes. Both anti-inflammatory and anti-malarial properties have been identified using appropriate assays, but the effective doses have demonstrated toxic effects for the experimental animals. Most of the pharmacological activities have been attributed to sesquiterpene lactones (STLs) and some chlorogenic acid derivatives (CAs) in the leaves of this species. This work aimed to evaluate the repeated-dose toxicity of an aqueous extract (AE) from Tithonia diversifolia leaves and to compare the results with an extract rich in STLs (LRE) and a polar extract (PE) without STLs but rich in CAs. The purpose of this work was to provide insights into the identity of the compounds responsible for the toxic effects of Tithonia diversifolia. The major classes of compounds were confirmed in each extract by IR spectra and HPLC-UV-DAD profiling using previously isolated or standard compounds. The toxicity of each extract was evaluated in a repeated-dose toxicity study in Wistar rats for 90 days. The AE is composed of both STLs and CAs, the LRE is rich in STLs, and the PE is rich in CAs. The AE caused alterations in haematological parameters but few alterations in biochemical parameters and was relatively safe at doses lower than 100mg/kg. However, the PE and LRE demonstrated several adverse effects by damaging the liver and kidneys, respectively. STLs and CAs can be toxic in prolonged use at higher doses in extracts prepared from Tithonia diversifolia by affecting the kidneys and liver. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  20. Analysis of Leucaena mimosine, Acacia tannins and total phenols by near infrared reflectance spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Prasad, M N.V. [Hyderabad Univ. (India). Dept. of Plant Sciences

    1995-11-01

    The mimosine contents of Leucaena foliage, Acacia tannins and total phenols from leaf, bark and pod were analyzed by a near infrared relectance spectrophotometer (Compscan 3000). A calibration equation (linear summation regression) was developed with near infrared spectral analysis software, using 30 spectra from old and young leaves of Leucaena and 23 spectra from different samples of Acacia. The near infrared analyzer calculated that the percentages of mimosine, total phenols and tannins are closely comparable to laboratory results. (author)

  1. Biological activities from extracts of endophytic fungi isolated from Viguiera arenaria and Tithonia diversifolia.

    Science.gov (United States)

    Guimarães, Denise O; Borges, Warley S; Kawano, Cristina Y; Ribeiro, Patrícia H; Goldman, Gustavo H; Nomizo, Auro; Thiemann, Otávio H; Oliva, Glaucius; Lopes, Norberto P; Pupo, Mônica T

    2008-01-01

    A total of 39 endophytic fungi have been isolated from Viguiera arenaria and Tithonia diversifolia, both collected in São Paulo State, Brazil. The isolates were identified based on their ribosomal DNA sequences. The ethyl acetate (EtOAc) extracts of all endophytic fungi were evaluated for their antimicrobial, antiparasitic and antitumoral activity. Antimicrobial screening was conducted using an agar diffusion assay against three pathogenic microorganisms: Staphylococcus aureus, Escherichia coli and Candida albicans. Antiparasitic activity was determined by enzymatic inhibition of gGAPDH of Trypanosoma cruzi and adenine phosphorybosiltransferase (APRT) of Leishmania tarentolae. Antitumoral activity was tested against human T leukemia cells by the Mosmann colorimetric method. All extracts showed activity in at least one assay: 79.5% of the extracts were cytotoxic against leukemia cells, 5.1% of the extracts were active against S. aureus, 25.6% against E. coli and 64.1% against Candida albicans. Only one extract showed promising results in the inhibition of parasitic enzymes gGAPDH (95.0%) and three were found to inhibit APRT activity. The cytotoxic extract produced by the strain VA1 (Glomerella cingulata) was fractionated and yielded nectriapyrone and tyrosol. Nectriapyrone showed relevant cytotoxic activity against both human T leukemia and melanoma tumor cell lines.

  2. Effect on of in vitro fermentation of mixture of Tithonia diversifolia, Cenchrus clandestinum and polyunsaturated fats

    Directory of Open Access Journals (Sweden)

    Juan Leonardo Cardona Iglesias

    2017-04-01

    Full Text Available The aim of this study was evaluated by in vitro, the effect of mixing wild sunflower (Tithonia diversifolia, kikuyu (Cenchrus clandestinum and concentrated feed, with the addition of polyunsaturated fatty acids (AGPI on the production of methane (CH4 ml/g MSd, dry matter digestibility (%DIVMS and production of volatile fatty acids (AGV. The research was conducted in NUTRILAB laboratory (Medellin, Colombia in September 2015. Three combinations of AGPI rich lipid sources were used with a total maximum inclusion level corresponding to 3% of incubated dry matter. Combinations of lipid sources were: SAGPI1: 0.5% soya oil, 0.5% sish oil, 2% rich in omega 3 bypass fat. SAGPI2: 1% Soya oil, 0.5% fish oil, 1.5% bypass fat rich in omega 3. SAGPI3: 2.5% Soya oil, 0.5% fish oil. A source of bypass fat (GSP was also used. The results were processed using pre-planned test comparisons through the PROC MIXED-SAS.The methane production in vitro decreased (p<0.05, while the dry matter digestibility increased (p<0.05 24 and 48 hours after wild sunflower was include and / or AGPI or both into the mixtures. In the molar ratio of AGV (%, acetic acid showed an increased presence. The inclusion of forage like wild sunflower and sources of AGPI supplementation in cattle, could be a strategy to reduce methane emissions without causing a detriment to the ruminal fermentation.

  3. Effects of Aqueous Shoot Extract of Tithonia diversifolia on the Growth of Seedlings of Monodora tenuifolia (Benth., Dialium guineense (Willd. and Hildegardia barteri (Mast. Kosterm

    Directory of Open Access Journals (Sweden)

    SAMSON OLAJIDE OKE

    2011-05-01

    Full Text Available The allelopathic effects of fresh shoot aqueous extract of Tithonia diversifolia (Hemsl. A.Gray, an invasive species introduced into Africa from North and Central America, on the growth of seedlings of three woody species: Monodora tenuifolia, Dialium guineense and Hildegardia barteri were investigated. The fresh shoot aqueous extract of Tithonia diversifolia was applied to the established seedlings of the three woody species over a period of ten weeks. The fresh shoot aqueous extract of Tithonia diversifolia had a significant effect (inhibitory and stimulatory on growth parameters such as shoot hThe allelopathic effects of fresh shoot aqueous extract of Tithonia diversifolia (Hemsl. A. Gray, an invasive species introduced into Africa from North and Central America, on the growth of seedlings of three woody species: Monodora tenuifolia, Dialium guineense and Hildegardia barteri were investigated. The fresh shoot aqueous extract of Tithonia diversifolia was applied to the established seedlings of the three woody species over a period of ten weeks. The fresh shoot aqueous extract of Tithonia diversifolia had a significant effect (inhibitory and stimulatory on growth parameters such as shoot height, leaf area, number of leaves and chlorophyll content of the three woody species.The study revealed that the fresh shoot aqueous extract of Tithonia diversofolia have different effects (inhibitory and stimulatory on the seedlings and the mode of action depends on the associated woody plant species.eight, leaf area, number of leaves and chlorophyll content of the three woody species.The study revealed that the fresh shoot aqueous extract of Tithonia diversofolia have different effects (inhibitory and stimulatory on the seedlings and the mode of action depends on the associated woody plant species.

  4. Thyroxine (T 4-RIA) and triiodothyronine (T 3-RIA) serum levels in sheep fed on Leucaena Leucocephala; Niveis sericos de tiroxina (T4-RIA) e triiodotinonina (T3-RIA) em ovinos alimentados cm Leucaena Leucocephala LAM

    Energy Technology Data Exchange (ETDEWEB)

    Pessoa, J M; Rodriguez, N M [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Escola de Veterinaria; Cardoso, W M [Maranhao Univ., Sao Luis, MA (Brazil). Escola de Medicina Veterinaria; Velez, C E.S.

    1988-12-31

    The aim of this work is to study the toxicity of Leucaena leucocephala on thyroxine and triiodothyronine serum concentration. Results indicate that ovine T 4 and T 3 serum levels do not decrease with Leucaena leucocephala feeding, sohen proreided for 41 days. (author). 10 refs, 1 tab.

  5. Kandungan Xantofil Daun Lamtoro (Leucaena leucocephala Hasil Detoksikasi Mimosin Secara Fisik dan Kimia

    Directory of Open Access Journals (Sweden)

    E. B. Laconi

    2010-04-01

    Full Text Available Leucaena (Leucena leucocephala is a feed source which has high protein and carotenoid, but its utilization as feed was limited by the presence of mimosine (β-N-(3-Hydroxy-4 pyridone-α amino propenoic acid as a toxin. Experiment was carried out using completely randomized design with 4 detoxification treatments and 5 replications. Detoxification treatments of leucaena leaf consisted of (1 non treated leucaena leaf, (2 steamed heating at 70 oC for 15 minutes, (3 dry heating at 70 oC for 12 h, (4 soaking with fresh water for 12 h, (5 spray with NaOH 5%, incubated for 12 h. Variables measured were mimosine and xanthophylls contents. Data were analyzed using analysis of variance and duncan multiple range test was further done to test the significant differences between means. The results showed that soaking leucaena leaf with fresh water for 12 h gave the highest reduction for both mimosine and xanthophylls contents and steamed heating at 70oC for 15 minutes had the highest xanthophylls content post detoxification. It was concluded that steamed heating at 70oC for 15 minutes was the best detoxification methods for mimosine in leucaena leaf.

  6. In vitro degradability test on rice bran containing Tithonia diversifolia (HEMSL. A. Gray and kelor (Moringa oleifera, LAMK leaves

    Directory of Open Access Journals (Sweden)

    Firsoni

    2010-10-01

    Full Text Available A research was done to investigate the advantages of Tithonia diversifolia (Hemsl. A. Gray and Moringa oleifera Lamk leaves as protein source in ruminant concentrate on in-vitro rumen metabolism. Randomized Block Design with 4 treatments and 5 replications was applied in this experiment. The treatments were: A = Tithonia diversifolia (TD 75% + Rice Bran (DD 25%: B = TD (56.25% + Moringa oleifera (MO 18.75% + DD 25%; C = TD 37.50% + MO 37.50% + DD 25%; D = TD 18.75% + MO 56.25% + DD 25%. Samples were weighted 375 ± 5 mg, placed into syringe glass 100 ml, 30 ml rumen liquor with bicarbonat buffer media was added and incubated in 390C for 48 hours. Parameters measured were gas production after 0, 2, 4, 6, 12, 24, and 48 hours incubation, degradability of organic matter (DBK and dry matter (DBO, NH3, total VFA concentration, and microbe biomass production (mg after 48 hours incubation. Results showed that Moringa oleifera replaced some Tithonia diversifolia in concentrate could improve gas production, total VFA concentration, DBK and DBO significantly (P < 0.05. The highest gas production was obtained from treatment D (52.98 ml/375 mg DM and the lowest was from treatment A (41,02 ml/375 mg DM. The highest VFA total was produced by treatment D (88.20 mM and the lowest was from treatment A (78.86 mM. The highest DBK dan DBO were obtained from treatment D (73.74 and 73.32% and the lowest was from treatment A (68.77 and 67.54%. Treatment D produced the highest NH3 and microbial biomass (38.09 mg/100 ml and 89.50 mg, the lowest was obtained from treatment A (35.84 mg/100 ml and 84.66 mg.

  7. Thyroxine (T 4-RIA) and triiodothyronine (T 3-RIA) serum levels in sheep fed on Leucaena Leucocephala

    International Nuclear Information System (INIS)

    Pessoa, J.M.; Rodriguez, N.M.; Cardoso, W.M.; Velez, C.E.S.

    1988-01-01

    The aim of this work is to study the toxicity of Leucaena leucocephala on thyroxine and triiodothyronine serum concentration. Results indicate that ovine T 4 and T 3 serum levels do not decrease with Leucaena leucocephala feeding, sohen proreided for 41 days. (author). 10 refs, 1 tab

  8. Preliminary note on strength properties of Leucaena leucocephala from lalkua, Tarai Bhabar Forest Division, uP

    Energy Technology Data Exchange (ETDEWEB)

    Shukla, N.K.

    1982-03-01

    Based on physical and mechanical tests conducted on small clear specimens, the strength properties of Leucaena leucocephala in green condition are reported and compared with Fraxinus micrantha, Morus alba and Tectona grandis. In general, Leucaena leucocephala has given slightly lower strength properties except in some cases as compared to above species. (Refs. 6).

  9. Novel anthraquinone derivatives produced by Phoma sorghina, an endophyte found in association with the medicinal plant Tithonia diversifolia (Asteraceae)

    International Nuclear Information System (INIS)

    Borges, Warley de Souza; Pupo, Monica Tallarico

    2006-01-01

    Three known anthraquinones (1,7-dihydroxy-3-methyl-9,10-anthraquinone, 1,6-dihydroxy- 3-methyl-9,10-anthraquinone and 1-hydroxy-3-methyl-9,10-anthraquinone), one new anthraquinone (1,7-dihydroxy-3-hydroxymethyl-9,10-anthraquinone), and two new hexahydro anthraquinone derivatives, dendryols E and F, were isolated from the culture of the endophytic fungus Phoma sorghina, found in association with Tithonia diversifolia (Asteraceae). Their structures were identified on the basis of spectroscopic data, mainly 1D and 2D NMR. (author)

  10. Novel anthraquinone derivatives produced by Phoma sorghina, an endophyte found in association with the medicinal plant Tithonia diversifolia (Asteraceae)

    Energy Technology Data Exchange (ETDEWEB)

    Borges, Warley de Souza; Pupo, Monica Tallarico [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Ciencias Farmaceuticas]. E-mail: mtpupo@fcfrp.usp.br

    2006-09-15

    Three known anthraquinones (1,7-dihydroxy-3-methyl-9,10-anthraquinone, 1,6-dihydroxy- 3-methyl-9,10-anthraquinone and 1-hydroxy-3-methyl-9,10-anthraquinone), one new anthraquinone (1,7-dihydroxy-3-hydroxymethyl-9,10-anthraquinone), and two new hexahydro anthraquinone derivatives, dendryols E and F, were isolated from the culture of the endophytic fungus Phoma sorghina, found in association with Tithonia diversifolia (Asteraceae). Their structures were identified on the basis of spectroscopic data, mainly 1D and 2D NMR. (author)

  11. Management and quantification of nitrogen fixation in Leucaena leucocephala

    International Nuclear Information System (INIS)

    Safo, E.Y.

    1998-01-01

    The effects of pruning and age on N 2 fixation were studied using Leucaena leucocephala isoline K28, with Cassia siamea as the non-N 2 -fixing reference species, at a site of degraded soil fertility typical of much of the farming land in Ghana. The 15 N-dilution method was used to estimate N2 fixation. Cassia siamea consistently produced higher total biomass and total N yields than did L. leucocephala. The mean value for the fraction of N derived from fixation (%Ndfa) was higher for pruned L. leucocephala (36%) than for unpruned trees (18%). There was some underestimation of N 2 fixation as a result of using C. siamea as the reference, and because root N-contents were not determined. Strong, significant linear correlations were observed between foliar and whole-tree (weighted average) percent 15 N atom excess in unpruned L. leucocephala and C. siamea, suggesting that foliar 15 N enrichment can be used to accurately estimate %Ndfa. The results demonstrated that the 15 N-enrichment methodology can provide meaningful estimates of %Ndfa and total N 2 fixed for mixed tree plantations under field conditions, when adequate spacing is provided. (author)

  12. Valor nutritivo e utilização do feno de leucena (Leucaena leucocephala cv. Cunningham para coelhos em crescimento Nutritional feeding value and use of Leucaena hay (Leucaena leucocephala cv. Cunningham for growing rabbits

    Directory of Open Access Journals (Sweden)

    Denise Fontana Figueiredo

    2000-05-01

    Full Text Available Dois experimentos foram realizados com o objetivo de avaliar a utilização do feno de leucena (Leucaena leucocephala cv.Cunningham para coelhos em crescimento. No ensaio de digestibilidade foram utilizados 28 coelhos da raça Nova Zelândia Branco, com 40 dias de idade, distribuídos em um delineamento experimental inteiramente casualizado, com dois tratamentos e 14 repetições. Foram utilizadas duas dietas, uma referência e uma teste na qual o feno de leucena substituiu 25% da matéria seca da dieta-referência. Os teores de matéria seca digestível, matéria orgânica digestível, proteína digestível, fibra em detergente neutro digestível e energia digestível do feno de leucena foram, respectivamente, de 26,87%; 25,30%; 5,32%; 14,66% e 1.286Kcal/kg. No experimento de desempenho foram utilizados 80 coelhos da raça Nova Zelândia Branco, de 35 a 70 dias de idade. Os animais foram distribuídos num delineamento experimental inteiramente casualizado, com cinco tratamentos (cinco níveis de inclusão de feno de leucena: 0, 25, 50, 75 e 100%, em substituição ao feno de alfafa, oito repetições e dois animais por unidade experimental. Os níveis crescentes de substituição do feno de alfafa pelo feno de leucena, não afetaram o desempenho dos coelhos durante o período experimental. Os dados mostram que o feno de leucena substituiu eficientemente o feno de alfafa em dietas para coelhos em crescimento.Two trial were carried out to determine the performance of rabbits fed on diets which included Leucaena hay (Leucaena leucocephala cv. Cunninghan. Twenty-eight 40-day old White New Zealand rabbits were employed for the digestibility trial, in a completely randomized design with two treatments and 14 replications. Reference and test diets were used in which Leucaena hay replaced 25% of the dry matter in the reference diet. Digestible dry matter, digestible organic matter, digestible protein, digestible F.D.N. and digestible energy contents of

  13. Utilización de la leucaena leucocephala como suplemento en cabras en crecimiento

    OpenAIRE

    Clavero, Tyrone; Razz, Rosa

    2009-01-01

    Con el objeto de evaluar el crecimiento de cabras mestizas (Nubian x Criollo) suplementadas con Leucaena leucocephala, se realizó un ensayo en el Occidente de Venezuela en una zona caracterizada como Bosque muy Seco Tropical. Se evaluaron tres raciones: T1 (pastoreo en gramínea-Cenchrus cillaris), T2 (pastoreo en gramínea + 2 h/día en Leucaena), T3 (pastoreo en gramínea + alimento concentrado comercial, 300 g/animal/día). El diseño experimental fue de bloques completamente al azar. El peso de...

  14. Six month-duration Tephrosia vogelii Hook.f. and Tithonia diversifolia (Hemsl.). A.Gray planted-fallows for improving maize production in Kenya

    NARCIS (Netherlands)

    Rutunga, V.; Karanja, N.K.; Gachene, C.K.K.

    2008-01-01

    An experiment including planted Tephrosia vogelii and Tithonia diversifolia fallow species and natural fallow was conducted at Maseno, Kenya, for assessing whether these fallows grown on a nutrient depleted land could produce sufficient green manure in six month period, whether their biomass

  15. Soil Seed Bank Dynamics in Tithonia diversifolia Dominated Fallowland Vegetation in Ile-Ife Area of Southwestern Nigeria

    Directory of Open Access Journals (Sweden)

    Samson Olajide OKE

    2009-12-01

    Full Text Available The soil seedbank of Tithonia diversifolia, an invasive species which dominates open waste fallowland vegetation was studied. Two different roadside sites which vary in extent of open waste land were selected.The species composition of the established vegetation was assessed in the two diferent sites. Twenty top soil samples were collected at five different distances (15 cm, 30 cm, 45 cm, 60 cm, and 75 cm inwards away from each main road in dry and rainy seasons and the seed bank composition was determined by greenhouse germination over a 6 month period. The similarity between the composition of the seed bank flora and that of the established vegetation was low. The least and the highest emerged seedlings density was recorded in the 15 metres and 75 metres respectively inwards away from the main road in both seasons. The results of the seedlings emergence is a reflection of the extent of open waste land dominated by the invasive species due to human disturbance (road construction on both sites. Overall results suggest that the emergence of the species from the soil seed bank may be due to the impact of the invasive species Tithonia diversifolia on other plant species in the study environment.

  16. Tithonia diversifolia: especie para ramoneo en sistemas silvopastoriles y métodos para estimar su consumo.

    Directory of Open Access Journals (Sweden)

    Estefanía Mejía-Díaz

    2016-12-01

    Full Text Available El objetivo del presente trabajo fue recopilar avances investigativos sobre aspectos agronómicos, nutricionales y productivos de T. diversifolia Hemsl. A Gray en sistemas silvopastorils (SSP como estrategia de producción ganadera y conocer metodologías para estimar el consumo de forraje de vacas bajo SSP. La introducción de sistemas silvopastoriles proporciona una vegetación estrati cada, además de generar diversos bienes, servicios ambientales y bienestar animal, constituyéndose, desde el punto de vista productivo, ecológico, económico y social, en una de las modalidades más prometedoras de los sistemas agroforestales. En la presente revisión se realizó una descripción de la arbustiva T. diversifolia Hemsl. A Gray, como especie estratégica para ser introducida en los sistemas silvopastoriles sean estos de baja o alta densidad, la cual por su calidad composicional ha sido reportada como una arbustiva con potencial para ser utilizada en la nutrición de rumiantes, teniendo un posible efecto positivo sobre índices productivos y económicos. Se discutieron tres técnicas utilizadas para estimar el consumo de forraje por bovinos en pastoreo, las cuales pueden ser aplicadas tanto en sistemas tradicionales como en sistemas silvopastoriles.

  17. Biolarvacidal activity of methanol extract of Kipahit leaves (Tithonia diversifolia against larvae of Chrysomya bezziana fly

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    Wardhana AH

    2014-03-01

    Full Text Available Myiasis or “belatungan” is the infestation of live human and vertebrate animal tissue with dipterous larvae, Chrysomya bezziana. In general, synthetic insecticides were applied to control the disease. However, it causes negative impact on animal product, so that it is required to find an alternative treatment using herbal medicine. The aim of this study was to access activity of methanol extract of Kipahit leaves (Tithonia diversifolia against various stages of C. bezziana larvae (L1, L2 and L3. Five treatments were applied with five replications, i.e. control/water (P0, 0,5%, 1%, 2% of the extract for PI, PII and PIII, respectively. Another treatment was 0,05% Asuntol for positive control (PIV. Each treatment was added with1% DMSO and twenty larvae were examined for each replication. Bioassay of L1 and L2 was addressed to investigate effect of intestinal toxicity by mixing the extract with Meat-Blood Mixture (MBM and Larval Rearing Media (LRM for L1 and L2, respectively. Bioassay of L3 was to investigate effect of contact toxicity through soaking the larvae into the extract solution for 10 seconds followed by incubating in vermiculite at 36oC. All living larvae after being treated by various concentration of the extract were reared to pupae and allowed to emerge as imago. Number of living larvae and pupae, pupae weight and number of imago were observed. All data were analysed using ANOVA followed by Dunnett test at 95% confidential limit. For L2, larval mortality were counted and probit analysed using POLO-PC software, therefore the lethal concentration (LC50 and LC95 and lethal time (LT50 and LT95 were defined. Results demonstrated that 1% of the extract was the most effective concentration which was able to kill the larvae and decrease the pupae weight. It also caused to fail pupation and imago emergence. The further study might be pursued to investigate in vivo assay of the extract in infested livestock.

  18. Changes in dinitrogen fixation in maturing stands of Casuarina equisetifolia and Leucaena leucocephala

    Science.gov (United States)

    John A. Parrotta; Dwight D. Baker; Maurice Fried

    1996-01-01

    Biological dinitrogen fixation in Casuarina equisetifolia J .R. & G. Forst. and Leucaena leucocephala (Lam.) de Wit was evaluated using the 15N-enrichment technique under field conditions in single-species and mixed-species plantings (with a nonfixing reference species, Eucalyptus X ...

  19. Estimation of nitrogen fixation in Leucaena leucocephala using 15N-enrichment methodologies

    Science.gov (United States)

    John A. Parrotta; Dwight D. Baker; Maurice Fried

    1994-01-01

    An estimation of biological nitrogen fixation by Leucaena leucocephala (Lam.) de Wit in monoculture and mixed-species plantations (with Casuarina equisetifolia L. ex J.R. & G. Forst., and Eucalyptus robusta Sm.) was undertaken over a two-year period in Puerto Rico using the 15N-enrichment...

  20. Leucaena toxicity: a new perspective on the most widely used forage tree legume

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    Michael J. Halliday

    2013-09-01

    Full Text Available The tree legume Leucaena leucocephala (leucaena is a high quality ruminant feed, vitally important for livestock production in the tropics, despite the presence of mimosine in the leaves. This toxic non-protein amino acid has the potential to limit productivity and adversely affect the health of animals. In the 1980s, the ruminal bacterium Synergistes jonesii was discovered and subsequently distributed in Australia as an oral inoculum to overcome these toxic effects. However, in recent times, a number of factors, including: surveys of the status of toxicity worldwide; improved understanding of the chemistry and mode of action of the toxins; new techniques for molecular sequencing; and concerns about the efficacy of the in vitro inoculum; have cast doubt on some past understanding of leucaena toxicity and provide new insights into the geographical spread of S. jonesii. There is also confusion and ignorance regarding the occurrence and significance of toxicity in many countries worldwide. Ongoing research into the taxonomy and ecology of the Synergistetes phylum, improved methods of inoculation, and improved management solutions, along with aware-ness-raising extension activities, are vital for the future success of leucaena feeding systems.

  1. Ovarian activity and estrus behavior in early postpartum cows grazing Leucaena leucocephala in the tropics.

    Science.gov (United States)

    Bottini-Luzardo, Maria; Aguilar-Perez, Carlos; Centurion-Castro, Fernando; Solorio-Sanchez, Francisco; Ayala-Burgos, Armin; Montes-Perez, Ruben; Muñoz-Rodriguez, David; Ku-Vera, Juan

    2015-12-01

    The legume Leucaena leucocephala (Leucaena) is widely used to supplement forage in silvopastoral livestock systems in Latin America. Little is known about its possible effects on the cow reproductive dynamic. The aim was to evaluate the effect of Leucaena foliage intake on re-establishment of ovarian activity and estrus behavior in early postpartum (7-90 days) cows. Twenty-four multiparous Bos taurus × Bos indicus cows were divided into two homogenous groups and assigned to one of two treatments: a silvopastoral system (SS, n = 12), consisting of an association of Cynodon nlemfuensis grass and L. leucocephala; and a control system (CS, n = 12), consisting of C. nlemfuensis alone. Intake of Leucaena in the SS ranged from 3.80 to 6.43 kg DM/cow/day. Plasma mimosine concentrations ranged from 1270 to 1530 μg/mL, and those for 2,3-dihydroxypyridine (DHP) from 147 to 729 μg/mL. No 3,4-DHP was detected in plasma. No difference (P > 0.05) between treatments was observed for the number of cows exhibiting small, medium, or dominant follicles, or estrus behavior. The number of cows which re-established ovarian cyclicity (n = 6) was lower (P < 0.05) in the SS than in the CS (n = 9). Corpus luteum lifespan was longer (P < 0.05) in the SS than in the CS. Intake of Leucaena affected the number of cows exhibiting ovarian cyclicity and extended corpus luteum life, but did not affect follicular development and estrus behavior.

  2. Tithonia diversifolia (Hemsl.) A. Gray as a medicinal plant: A comprehensive review of its ethnopharmacology, phytochemistry, pharmacotoxicology and clinical relevance.

    Science.gov (United States)

    Mabou Tagne, Alex; Marino, Franca; Cosentino, Marco

    2018-06-28

    Tithonia diversifolia (TD) is widely valued in several cultures for its medicinal properties. A comprehensive review of the current understanding of this plant species is required due to emerging concerns over its efficacy, toxicity and allergenic potential. We critically summarized the current evidence on the botany, traditional use, phytochemistry, pharmacology and safety of TD, with the view to provide perspectives for developing more attractive pharmaceuticals of plant origin, but also to lay a new foundation for further investigations on this plant. A preliminary consultation of search engines such as Web of Science, PubMed, ScienceDirect and other published/unpublished resources provided an overview of extant literature on TD. Then, we meticulously screened all titles, abstracts and full-texts to establish consistency in the application of inclusion criteria. Studies were considered for inclusion if they dealt with taxonomy, global distribution, local and traditional knowledge, phytochemistry, toxicity and biological effects. 1856 articles were retrieved among which 168 were revised and included. Several studies conducted on cell lines and animals provided supporting evidence for some ethnomedicinal claims of extracts from TD. Short-term use of Tithonia extracts were effective and well-tolerated in animals when taken at lower doses. Both the toxic and therapeutic effects were attributed to bioactive principles naturally occurring in this species including sesquiterpene lactones, chlorogenic acid and flavonoids. T. diversifolia is a valuable source of bioactive compounds with significant therapeutic implications and favourable safety index. However, more rigorously designed investigations are needed to recommend the whole plant or its active ingredients as a medication, and should focus on understanding the multi-target network pharmacology of the plant, clarifying the effective doses as well as identifying the potential interactions with prescribed drugs or

  3. Potencial forrajero de Tithonia diversifolia Hemsl. A Gray en la producción de vacas lecheras

    Directory of Open Access Journals (Sweden)

    Luis Alberto Gallego-Castro

    2014-07-01

    Full Text Available El objetivo de este trabajo fue analizar el uso potencial de la Tithonia diversifolia (Hemsl. A. Gray (botón de oro en la alimentación de vacas lecheras en el trópico alto colombiano. Se eligieron términos clave para la búsqueda de información y a partir de ellos se abordaron y analizaron diferentes publicaciones, permitiendo un acercamiento a la problemática propuesta. En estos sistemas de producción típicos del trópico alto en Colombia, el kikuyo (Pennisetum clandestinum contribuye con el mayor aporte en la ración del ganado y debido al alto N, la baja fibra y materia seca, lleva con frecuencia a balances energéticos negativos en las vacas más productivas, por lo que en muchos casos se sostiene la producción con alimento comercial, compuesto principalmente por cereales y con altos niveles de proteína. Las necesidades nutricionales de este tipo de sistemas productivos están orientados a encontrar estrategias que permitan mejorar la oferta forrajera, en términos de variedad y calidad, disminuir la dependencia de alimentos comerciales o al menos facilitar la inclusión de otros que mejoren el desempeño animal. A partir de este análisis, se evidencia el potencial de T. diversifolia en la alimentación de vacas lecheras de alta producción; esta forrajera arbustiva, por su contenido de proteína, carbohidratos solubles y taninos, puede tener un impacto positivo sobre los sistemas de ganadería lechera intensiva y puede incorporarse a suplementos alimenticios.

  4. IN VITRO FERMENTATION EFFICIENCY OF MIXTURES OF Cynodon nlemfuensis, Leucaena leucocephala AND TWO ENERGY SOURCES (MAIZE OR SUGAR CANE MOLASSES

    Directory of Open Access Journals (Sweden)

    Juan Martin Estrada-Liévano

    2009-07-01

    Full Text Available The in vitro fermentation efficiency of Cynodon nlemfuensis forage (star grass and Leucaena leucocephala foliage (leucaena and two energy sources (i.e. maize and sugar cane molasses mixture was evaluated. Mixture samples (1 g DM were incubated for 24 h. All the mixtures were added with 500 mg of polyetilenglycol (PEG. Adding molasses to star grass increased dry matter true digestibility and carbohydrate fermentation (P

  5. Effects of Leucaena pallida and Sesbania sesban supplementation on testicular histology of tropical sheep and goats

    NARCIS (Netherlands)

    Woldemeskel, M.; Tegegne, A.; Umunna, N.N.; Kaitho, R.J.; Tamminga, S.

    2001-01-01

    Thirty Ethiopian highland rams with an average body weight of 23.7 kg (S.D.=1.23) and age of 18 months and 25 East African bucks with an average body weight of 18.6 kg (S.D.=2.06) and age of 14 months were used to study the long term effects of supplementation with the leaves of Leucaena pallida and

  6. Efecto bioestimulante de Trichoderma harzianum Rifai en posturas de Leucaena, Cedro y Samán

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    Leonides Castellanos González

    2018-01-01

    Full Text Available El presente artículo evaluó el efecto bioestimulante de Trichoderma harzianum Rifai en la producción de postura de Leucaena leucocephala (Lam de Wit., Cedrela odorata L. y Albizia saman (Jacq. Merr. Se desarrollaron tres experimentos en condiciones de vivero, uno para cada especie. Se empleó un diseño completamente aleatorio con cuatro parcelas por tratamiento. En cada experimento los tratamientos fueron: Trichoderma harzianum a razón de 20 g.L-1, 40 g.L-1 y un testigo. Se evaluó el porcentaje de germinación y las variables morfométricas diámetro y altura del tallo, así como biomasa seca en raíz y parte aérea de la planta. Los tratamientos con Trichoderma no incrementaron el porcentaje de germinación en cedro, samán y leucaena. T. harzianum incrementó la altura, el número de hojas y la biomasa seca del área foliar en las plántulas de cedro, mientras que en leucaena y samán solo provocó incrementos del diámetro basal de las plántulas.

  7. Nitrogen fixation in Leucaena leucocephala and effects of pruning s on cereal yields

    International Nuclear Information System (INIS)

    Bekunda, M.

    1998-01-01

    Leucaena leucocephala was interplanted with reference tree species, Cassia siamea and Cassia spectabilis, and estimates of percent N derived from N 2 fixation (%Ndfa) were made, by the isotope-dilution method, at 4, 6, 14, 20 and 30 months after transplanting. The %Ndfa values were low and variable throughout the growth period, except after thinning at 14 months when there was a five-fold increase. The two non-fixing reference species outperformed the N 2 -fixing Leucaena in above-ground vegetative production, and provided different fixed-N estimates. Prunings from the L. leucocephala and C. Siamea trees were applied separately to soil as green manure. Maize was planted to test the effects of the Leucaena green manure on soil fertility, and millet was the test crop for the Cassia. Whether surface-applied or incorporated, the prunings significantly improved yields, which were generally similar among rates and methods of application. The proportions of cereal N obtained from prunings ranged from 8 to 33%, with no cereal-yield correlation. The data indicate that multipurpose tree prunings are of potential use to farmers as organic sources of nutrients, even at relatively low application rates, without need for incorporation into the soil. (author)

  8. Nitrogen fixation and effects of pruning on Gliricidia sepium and Leucaena leucocephala

    International Nuclear Information System (INIS)

    Liyanage, M.S. de

    1998-01-01

    This 7-year study examined genetic variability in N 2 fixation by Gliricidia sepium and the N 2 -fixing capacity in G. sepium and Leucaena leucocephala as influenced by frequency of pruning, age, and shade from coconut. The 15 N-dilution method was used with the non-nodulating tree legume Senna siamea as the non-fixing reference. There were significant differences in total dry matter, N yield and N 2 -fixation capacity among four G. sepium provenances. Gliricidia had higher values than Leucaena for dry matter, N yield, and amount of N fixed; %Ndfa was comparable in both species (47-55%). A substantial amount (18%) of fixed N 2 was present in the roots of both species. In a long-term study aimed at comparing the effect of pruning practices and age of trees, G. sepium grown under coconut outperformed L. leucocephala in terms of dry matter, N yield and amounts of N 2 fixation. Coconut saplings supplied with G. sepium and L. leucocephala prunings as green manure grew better than those supplied with S. siamea; the fraction of coconut-sapling N obtained from Gliricidia and Leucaena was 40 and 36%, respectively. These results suggest that G. sepium, which demonstrated a high potential for biomass production and N 2 fixation, is appropriate for interplanting with coconut palms. Also, S. Siamea was found to be a suitable reference species. (author)

  9. Effects of sulfur and phosphorus application on the growth, biomass yield and fuel properties of leucaena (Leucaena leucocephala (Lam. de Wit. as bioenergy crop on sandy infertile soil

    Directory of Open Access Journals (Sweden)

    Songyos Chotchutima

    2016-01-01

    Full Text Available A field experiment was conducted to determine the effect of Sulfur (S and Phosphorus (P fertilizer on the growth, biomass production and wood quality of leucaena for use as a bioenergy crop at the Buriram Livestock Research and Testing Station, Pakham, Buriram province, Thailand during 2011–2013. The experiment was arranged in a split plot design with two rates of S fertilizer (0 and 187.5 kg/ha as a main plot and five rates of P (0, 93.75, 187.5, 375 and 750 kg/ha as a sub-plot, with four replications. The results showed that the plant height, stem diameter, total woody stem and biomass yield of leucaena were significantly increased by the application of S, while the leaf yield was not influenced by S addition. The total woody stem and biomass yield were also proportionately greatest with the maximum rate of P (750 kg/ha application. The addition of S did not result in any significant differences in fuel properties, while the maximum rate of P application also showed the best fuel properties among the several rates of P, especially with low Mg and ash contents compared with the control (0 kg/ha.

  10. Analisis Karbohidrat, Protein, dan Lemak pada Pembuatan Kecap Lamtoro Gung (Leucaena leucocephala terfermentasi Aspergillus oryzae

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    TJAHJADI PURWOKO

    2005-05-01

    Full Text Available Leucaena leucocephala was one of Mimosaceae family which had high protein content nearly the same value as in Soya beans protein content. L. leucocephala could be used as the substitution of Soya beans in production of soy sauce. Soy sauce was one of fermentation liquid products which had flavor and aroma as meat, and had high nutrition value. The aims of the research were (i to analyze nutrition value in seeds, koji and moromi including carbohydrate, proteins and lipids and (ii to know the potency of L. leucocephala as raw material in production of soy sauce by fermentationmethod with Aspergillus oryzae as inoculums. Fermentation was one of soy sauce production processes that involved two steps: (i the solid stage fermentation and (ii the brine fermentation. L. leucocephala was fermented with A. oryzae for 3-5 days to produce koji. Koji was soaked in salt solution 20% (1:5 b/v then it was fermented for 30 days to produce moromi. Nutritional values in L. leucocephala seeds, koji, and moromi was analyzed, i.e. carbohydrate (sugar reduction and starch, protein, and lipids. Moromi filtrate was added by spices to Leucaena sauce. Finally, preferable Leucaena sauce was tested including flavor, aroma, and color. Preferable data was analyzed by non-parametric statistic; it was Friedman Test and followed Wilcoxon Ranking Method. The result showed that value of reducing sugar in L. leucocephala seeds, koji and moromi were 78.38 mg/g; 119.08 mg/g; and 164.29 mg/g. Starch value of seeds, koji and moromi were 274.36 mg/g; 260.92 mg/g and 179.50 mg/g. The value of dissolved protein in seeds, koji and moromi were 107.44 mg/g; 86.1 mg/g; and 208.56 mg/g. The value of lipids on seeds, koji and moromi was 158.87 mg/g; 51.35 mg/g; and 80.86 mg/g. The data of preferable test on Leucaena sauce’s taste was the same as ABC sauce but the aroma of Leucaena sauce had the lowest score than the others. More over, the four kinds of sauce were not different significantly

  11. Leucaena leucocephala (Lam. de Wit (Fabaceae: invasora ou ruderal? Leucaena leucocephala (Lam. de Wit (Fabaceae: invasive or ruderal?

    Directory of Open Access Journals (Sweden)

    José Nicola Martorano Neves da Costa

    2010-10-01

    Full Text Available A utilização de espécies exóticas em plantios de recuperação de áreas degradadas tem sido condenada com base no argumento de que tais espécies podem se comportar como invasoras e contaminar os ecossistemas naturais ao redor das áreas onde forem plantadas. Leucaena leucocephala (leucena é espécie leguminosa exótica que tem sido frequentemente cultivada no Brasil para recuperação florestal, uma vez que apresenta simbiose com bactérias fixadoras de nitrogênio, melhorando a fertilidade dos solos. O potencial de invasão e persistência da leucena foi analisado a partir de um plantio misto, efetuado em 1983 em terreno com afloramento rochoso, parte do mosaico de uma paisagem dominada por matriz agrícola, com alguns fragmentos remanescentes de floresta estacional semidecidual e plantios arbóreos diversos. Em uma área de 200 ha, cada uma das 11 unidades do mosaico foi amostrada por meio de seis parcelas de 16 x 3 m (48 m², em que foram identificados e medidos (DAP todos os indivíduos de espécies arbóreas (altura mínima de 50 cm, para verificar se a área ocupada pela espécie está se expandindo. Na área em que foi utilizada leucena no plantio, analisou-se a estrutura da comunidade, com base na distribuição dos indivíduos em classes de tamanho, para verificar se a proporção da espécie exótica tende a aumentar com o tempo, configurando a persistência no ecossistema. Uma vez que nenhum indivíduo de L. leucocephala foi registrado entre os 4.599 amostrados além dos limites da área onde a espécie foi plantada, a conclusão é de que a espécie não está se comportando como invasora de ecossistemas naturais nessa condição ambiental, enquadrando-se melhor como ruderal. A análise da comunidade em regeneração sob as árvores plantadas evidenciou que a proporção da espécie exótica, que é intolerante à sombra, tende a diminuir com o tempo, ainda que lentamente, perdendo espaço para espécies nativas tolerantes

  12. Características nutritivas del ensilaje de Leucaena leucocephala con diferentes aditivos

    OpenAIRE

    Betancourt de Flores, M.; Clavero, Tyrone; Razz, Rosa

    2009-01-01

    Para estudiar la influencia de la melaza y ácido fórmico sobre las características nutritivas del ensilaje de Leucaena leucocephala, se realizó un estudio en una zona caracterizada como Bosque muy seco tropical, estado Zulia, Venezuela. Tres niveles de melaza (0, 2.5 y 5%) y ácido fórmico (0, 0.25 y 0.5%) fueron evaluados, utilizándose un diseño experimental completamente aleatorizado con arreglo factorial 3x3. Se evaluaron los contenidos de materia seca total (MST), proteína cruda (PC)...

  13. Caracterización bromatológica de Tithonia diversifolia (Hemsl. A. Gray en dos etapas de su ciclo fisiológico Bromatological characterization of Tithonia diversifolia (Hemsl. A.Gray in two stages of its physiological cycle

    Directory of Open Access Journals (Sweden)

    Yohanka Lezcano

    2012-09-01

    Full Text Available El objetivo del estudio fue caracterizar bromatológicamente algunos componentes esenciales del valor nutritivo de Tithonia diversifolia (Hemsl. Grayen dos etapas del ciclo fisiológico (30 y 60 días, en los períodos lluvioso (PLL y poco lluvioso (PPLL. Se utilizó un diseño experimental completamente aleatorizado, con seis tratamientos derivados de la relación entre las fracciones comestibles (hojas, tallos tiernos y hojas más tallos tiernos y los momentos del ciclo fisiológico de la planta. Los indicadores medidos fueron: la materia seca (MS, la proteína bruta (PB, la fibra bruta (FB, el calcio (Ca, el magnesio (Mg y la ceniza. Para el análisis de varianza se utilizó el paquete estadístico SPSS versión 15.0 para Windows. El follaje de tithonia presentó variaciones en su calidad nutritiva. A los 30 días se encontraron los mejores valores de PB (29,79% y 28,69%, Mg (0,094% y 0,210% y ceniza (16,32% y 20,59% para el PLL y el PPLL, respectivamente, con resultados similares entre las fracciones comestibles para las dos etapas del ciclo fisiológico, en los dos períodos evaluados. El contenido de FB en los tallos tiernos a los 60 días fue el más representativo en ambos períodos (5,29 y 5,27%, respectivamente. Integralmente, la fracción hoja más tallo tierno alcanzó los mejores valores de los indicadores medidos para ser utilizada en la alimentación animal.The objective of the study was to characterize bromatologically some essential components of the nutritional value of Tithonia diversifolia (Hemsl. A. Gray in two stages of the physiological cycle (30 and 60 days, during the rainy (RS and dry seasons (DS. A completely randomized experimental design was used, with six treatments derived from the relationship between the edible fractions (leaves, fresh stems and leaves plus fresh stems and the moments of the plant physiological cycle. The indicators measured were: dry matter (DM, crude protein (CP, crude fiber (CF, calcium (Ca

  14. Milk yield and blood urea nitrogen in crossbred cows grazing Leucaena leucocephala in a silvopastoral system in the Mexican tropics

    Directory of Open Access Journals (Sweden)

    Maria B. Bottini-Luzardo

    2016-09-01

    Full Text Available The aim of the study was to assess milk yields, estimate the intake of crude protein (CP and determine the concentrations of blood urea nitrogen (BUN in early post-partum crossbred cows grazing irrigated Leucaena leucocephala (leucaena in a silvopastoral system relative to those in an irrigated grass monoculture.  Twenty-four multiparous cows were randomly allotted at calving on the basis of previous milk yields to 2 grazing treatments: grass monoculture system (MS of Cynodon nlemfuensis (n=12; and an intensive silvopastoral system (ISS composed of leucaena and C. nlemfuensis (n=12.  Cows were supplemented with sorghum grain (ISS or a conventional concentrate (MS during milking to ensure availability of metabolizable energy (ME and CP required for milk production.  Mean estimated intake of leucaena was 5.1±1.3 kg DM/d and estimated CP intakes were 1,479±3.3 and 1,258±3.3 g/d for ISS and MS, respectively (P>0.05, while estimated intakes of ME were 161±1.3 and 131±1.4 MJ/d for ISS and MS, respectively (P<0.05.  Milk yields were 13.5 and 14.5 kg/cow/d for cows on ISS and MS, respectively (P>0.05.  Concentrations of BUN were 19.1 mg/dL for cows in ISS and 15.3 mg/dL for cows in MS (P<0.05.  We conclude that intake of leucaena and sorghum grain in an irrigated silvopastoral system was sufficient to substitute for expensive concentrate in the diets of lactating cows grazing irrigated grass monoculture.  However, the higher levels of BUN found in ISS suggest a lower efficiency of N utilization in this treatment.  Restricting consumption of leucaena might be a means of improving efficiency of its use and this warrants investigation. Keywords: Cattle, crude protein, Cynodon nlemfuensis, leucaena, tropical pasturesDOI: 10.17138/TGFT(4159-167

  15. HPLC-DAD fingerprinting analysis, antioxidant activities of Tithonia diversifolia (Hemsl.) A. Gray leaves and its inhibition of key enzymes linked to Alzheimer's disease.

    Science.gov (United States)

    Ojo, Oluwafemi Adeleke; Ojo, Adebola Busola; Ajiboye, Basiru Olaitan; Olaiya, Oluranti; Okesola, Mary Abiola; Boligon, Aline Augusti; de Campos, Marli Matiko Anraku; Oyinloye, Babatunji Emmanuel; Kappo, Abidemi Paul

    2018-01-01

    Tithonia diversifolia (Hemsl.) A. Gray leaves have long been used to manage neurodegenerative diseases without scientific basis. This study characterized the phenolic constituents, evaluated the antioxidant properties of phenolic extracts from T. diversifolia leaves used as traditional medicine in Africa and its inhibition of key enzymes linked to Alzheimer's disease. The extract was rich in phenolic acids (gallic acid, chlorogenic acid, caffeic acid and p -coumaric acid) and flavonoids (apigenin) and had 1,1-diphenyl-2-picryl-hydrazil radical scavenging abilities (IC 50  = 41.05 μg. mL -1 ), 2,2-Azino-bis3-ethylbenthiazoline-6sulphonic acid radical scavenging ability (IC 50  = 33.51 μg. mL -1 ), iron chelation (IC 50  = 38.50 μg. mL -1 ), reducing power (Fe 3+ - Fe 2+ ) (7.34 AAEmg/100 g), inhibited acetylcholinesterase (IC 50  = 39.27 μg mL -1 ) and butyrylcholinesterase (IC 50  = 35.01 μg mL -1 ) activities. These results reveal the leaf as a rich source of phenolic compounds with antioxidant and cholinesterase inhibitory activity.

  16. Composión trófica de la comunidad insectil en dos agroecosistemas ganaderos con Leucaena leucocephala (Lam. de wit y Panicum maximum Jacq. Trophic composition of the insect community in two livestock production agroecosystems with Leucaena leucocephala (Lam. de wit and Panicum maximum Jacq.

    Directory of Open Access Journals (Sweden)

    O Alonso

    2011-12-01

    Full Text Available Con el objetivo de definir los principales grupos funcionales en la comunidad de insectos presentes en dos áreas compuestas por la asociación de Leucaena leucocephala cv. Perú y Panicum maximum cv. Likoni (un sistema silvopastoril y un campo de semilla, respectivamente, ambas localizadas en la Estación Experimental de Pastos y Forrajes "Indio Hatuey", se muestrearon cada 15 días, durante tres años, las hojas, las inflorescencias y las legumbres de la leguminosa y el follaje de la gramínea, para colectarlos. La clasificación de los grupos se realizó a partir de: la identificación de cada especie insectil, la información que ofrece la literatura acerca de su hábito principal de alimentación y las observaciones realizadas en el campo. Con estos elementos se definieron los fitófagos y los benéficos, y como subgrupos de estos últimos: los depredadores, los parasitoides, los polinizadores, los descomponedores de la materia orgánica, los coprófagos y los micófagos. En total se identificaron 113 especies de insectos, 63 con hábitos fitófagos y los 50 restantes benéficos. En el estrato arbóreo se encontraron 88 especies, 49 fitófagas (56% y 39 benéficas (44%; y 103 en el herbáceo, 59 insectos fitófagos (57% y 44 benéficos (43%; 78 especies coincidieron en los dos estratos. Se destaca que en ambos predominaron los depredadores y los parasitoides de los órdenes Hymenoptera, Coleoptera y Diptera, tales como: Cycloneda sanguinea limbifer Casey, Coccinella maculata (De Geer, Chilocorus cacti Linnaeus, Conura sp., Pimpla marginella (Brullé y Rogas sp. Se concluye que la estructura y función de la comunidad de insectos mostró un número relativamente mayor de insectos fitófagos con respecto a los benéficos; sin embargo, fue importante el predominio de los enemigos naturales, responsables de la actividad reguladora de las poblaciones de fitófagos, a las que mantienen por debajo del umbral de daño económico en el cultivo de la

  17. Leucaena and cassava tops as supplements for buffaloes fed local grass

    International Nuclear Information System (INIS)

    Hendratno, C.; Abidin, Z.; Suharyono; Bahauddin, R.; Yates, N.G.; Winogroho, M.

    1984-01-01

    Experiments were conducted using fistulated female buffaloes to determine the effect of increasing levels of high-protein forage (leucaena and cassava tops) on the intake of grass and its digestibility, and to study the mechanisms of action of such supplements based on measurements of a number of parameters of rumen function. Marked increases in intake were observed and these were associated with increases in the digestibility of diets supplemented with these forages. These responses were accompanied by increased rumen fermentation as indicated by ammonia-N and volatile fatty acid concentrations, and rates of microbial protein synthesis. The results suggest that local grass requires supplementation with fermentable N and bypass protein for efficient use by ruminants. (author)

  18. Characterization of Leucaena (Leucaena leucephala) oil by direct analysis in real time (DART) ion source and gas chromatography; Caracterización del aceite de Leucaena (Leucaena leucocephala) por análisis directo en tiempo real (DART) y cromatografía de gases

    Energy Technology Data Exchange (ETDEWEB)

    Alam, M.; Alandis, N.M.; Sharmin, E.; Ahmad, N.; Alrayes, B.F.; Ali, D.

    2017-07-01

    For the first time, we report the characterization of triacylglycerols and fatty acids in Leucaena (Leucaena leucephala) oil [LUCO], an unexplored nontraditional non-medicinal plant belonging to the family Fabaceae. LUCO was converted to fatty acid methyl esters (FAMEs). We analyzed the triacylglycerols (TAGs) of pure LUCO and their FAMEs by time-of-flight mass spectrometry (TOF-MS) followed by multivariate analysis for discrimination among the FAMEs. Our investigations for the analysis of LUCO samples represent noble features of glycerides. A new type of ion source, coupled with high-resolution TOF-MS was applied for the comprehensive analysis of triacylglycerols. The composition of fatty acid based LUCO oil was studied using Gas Chromatography (GC-FID). The major fatty acid components of LUCO oil are linoleic acid (52.08%) oleic acid (21.26%), palmitic acid (7.91%) and stearic acid (6.01%). A metal analysis in LUCO was done by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The structural elucidation and thermal stability of LUCO were studied by FT-IR, 1H NMR, 13C NMR spectroscopic techniques and TGA-DSC, respectively. We also measured the cytotoxicity of LUCO [Spanish] Se presenta por primera vez la caracterización de triacilgliceroles y ácidos grasos del aceite de Leucaena (Leucaena leucephala) [LUCO], una planta no medicinal, no tradicional y no explorada, perteneciente a la familia Fabaceae. Se analizaron triacilgliceroles (TAGs) de LUCO y sus FAMEs por espectrometría de masas de tiempo de vuelo (TOF-MS) seguido de análisis multivariante para discriminación entre los FAME. Nuestras investigaciones para el análisis de muestras de LUCO presentaron características propias de los glicéridos. Un nuevo tipo de fuente de iones, junto con alta resolución TOF-MS se aplicó para el análisis exhaustivo de triacilgliceroles. La composición de aceite de LUCO basado en ácidos grasos se estudió usando Cromatografía de Gas (GC-FID). Los principales

  19. Poblaciones microbianas ruminales en novillas alimentadas con Leucaena leucocephala en el Bosque Seco Tropical colombiano.

    Directory of Open Access Journals (Sweden)

    Erika Angarita

    2015-05-01

    Full Text Available La fermentación y metanogénesis ruminal son procesos metabolicos vitales para los bovinos y son llevados a cabo por poblaciones microbianas, las cuales se afectan por factores como la presencia de metabolitos secundarios, la composición nutricional y la degradabilidad de la dieta. El objetivo de este trabajo fue monitorear las poblaciones de bacterias totales, metanógenos totales y Butirivibrio fibrisolvens en el rumen de novillas raza Lucerna, alimentadas con dietas típicas de un sistema silvopastoril intensivo y un sistema tradicional. Para ello, se colectó contenido ruminal (CR por vía oral a ocho novillas que consumían 100% Cynodon plectostachyus (control y 76% C. plectostachyus + 24% Leucaena leucocephala siguiendo un diseño de sobre-cambio. A partir del CR se extrajo y cuantificó ADN mediante PCR cuantitativa. Las poblaciones [Log10 (ng/g CR] fueron 5.6 y 5.8 para bacterias totales (P= 0.5343, 3.6 y 3.5 para B. fibrisolvens (P= 0.4742, y 5.0 y 5.3 para metanógenos totales (P= 0.2661, para la dieta control y la dieta con leucaena respectivamente. Las poblaciones monitoreadas cuantitativamente no difirieron de manera significativa con la inclusión de L. leucocephala. Esto indica la importancia de investigar la estructura, función e interacciones de las poblaciones más allá del análisis cuantitativo para determinar cómo la dieta afecta las poblaciones microbianas ruminales y su función.

  20. Fertigation by capillary action in seedlings of Leucaena leucocephala (Lam. de Wit = Fertirrigação por capilaridade em mudas de Leucaena leucocephala (Lam. de Wit.

    Directory of Open Access Journals (Sweden)

    Renata de Paiva Dantas

    2016-07-01

    Full Text Available The leucena (Leucaena leucocephala, native to Central America, produces large quantities of viable seeds, which allows its propagation on a large scale; it is also fast-growing and can reach 7 m in height. The use of fertigation favours the absorption of nutrients, and the greater growth of the leucena seedlings, due to the application of less concentrated nutrient solutions of a consequently lower electrical conductivity. However, this practice requires a lot of manpower, and it is necessary to find alternative technologies that would allow a reduction in the costs of fertigation. The aim of this study therefore was to evaluate the viability of fertigation by capillary action, using different concentrations of a standard nutrient solution in the production of seedlings of leucena. A completely randomised experimental design was used, with treatments comprising four concentrations of nutrient solution (0, 50, 100 and 150% applied by capillary fertigation. Two nondestructive evaluations of growth were carried out at 25 and 40 days after sowing (DAS, and one destructive evaluation at 55 DAS. The variables to be evaluated were: plant height, stem diameter, number of leaves and biomass accumulation, besides the quality indices of the seedlings. Analysis of the data leads to the conclusion that seedlings of leucena can be produced at concentrations of between 70 and 100% of the nutrient solution through fertigation by capillary action. = A leucena (Leucaena leucocephala, originária da América Central, produz grandes quantidades de sementes viáveis, o que permite sua propagação em larga escala, e possui crescimento rápido, podendo chegar a 7 metros de altura. O uso da fertirrigação favorece a absorção de nutrientes e o maior crescimento das mudas de leucena, devido à aplicação de solução nutritiva menos concentrada e, consequentemente, com menor condutividade elétrica. Nesse sentido, objetivou-se com este trabalho avaliar a viabilidade

  1. Efecto de la altura de poda en Leucaena leucocephala y su influencia en el rebrote y rendimiento de Panicum maximum

    Directory of Open Access Journals (Sweden)

    H. M. Bacab

    2012-01-01

    Full Text Available Una de las prácticas importantes de manejo en los sistemas de Leucaena leucocephala asociada con pastos tropicales, como Panicum maximum, es la poda; en la actualidad se continúan los estudios para determinar los efectos de esta práctica en el componente arbóreo y herbáceo. Por todo ello, en el presente trabajo se evaluó el efecto de tres alturas de poda en Leucaena leucocephala cv. Cunningham y su influencia en la pastura asociada (Panicum maximum cv. Tanzania. El estudio se realizó en la época poco lluviosa del año (marzo a mayo de 2010, se utilizó un diseño de bloques completos al azar con cuatro repeticiones. Al inicio del experimento se asignaron los tratamientos (alturas de poda de 20, 40 y 60 cm sobre el nivel del suelo para Leucaena leucocephala. En el caso de Panicum maximum, se realizó una poda a 5 cm sobre el nivel del suelo en todas las parcelas experimentales. Los resultados obtenidos indicaron que la poda de la leguminosa a 40 y 60 cm permitió obtener brotes de mayor talla, sin afectar a la gramínea asociada. De igual manera, al incrementar la altura de poda, la leguminosa presentó mayor rendimiento y proporción de forraje comestible; sin embargo, se afectó negativamente a la pastura asociada. Ante ello, considerándose el aspecto animal, se recomienda no reducir la altura de poda de Leucaena leucocephala a menos de 40 cm, ya que se reduce significativamente el rendimiento y la proporción de forraje comestible de esta leguminosa, el cual es de mayor calidad para la alimentación animal.

  2. Forage production and growing goats’ response under silvopastoral systems based on Guazuma ulmifolia, Leucaena leucocephala and Crescentia cujete

    Directory of Open Access Journals (Sweden)

    Gustavo Rodríguez Fernández

    2013-01-01

    Full Text Available Grass monoculture, besides being unnatural to goat’s natural eating habits, exhibits low forage production during the dry season, with negative impacts on animal productivity. This research aimed to determine the productive advantages of silvopastoral system arrangements in goat production. A completely randomized design with repeated measurements through time was used. Six treatments were evaluated: kikuyina grass monoculture (Bothriochloa pertusa and guinea grass monoculture (Panicum maximum cv. Tanzania as control groups; guacimo (Guazuma ulmifolia based silvopastoral arrangement; calabash (Crescentia cujete based silvopastoral arrangement; lead tree (Leucaena leucocephala based silvopastoral arrangement; and a mixed based silvopastoralarrangement (guacimo, calabash and leucaena. The information was processed with analysis of variance. The results showed increased forage production in silvopastoral arrangements vs. Bothriochloa pertusa monoculture. The greater increase in height (p <0.05 at 9-14 months of age, was obtained with the leucaena silvopastoral arrangement. All silvopastoral arrangements showed forage yield advantages compared to B. pertusa. The higher dry matter production of guinea grass is highlighted. Overall weight gain of the growing goats was low; nevertheless, a differential response between treatments was observed. Silvopastoral arrangements had the highest (p <0.05 weight gain (22.5 to 33.6 g/animal per day relative to the guinea grass monoculture (13.2 g/animal per day. The growing goats had higher percentages of estrus and pregnancy in the mixed system (66.7% and those based on guacimo (66.7% and on lead tree (55.6%.

  3. Caracterización cultural y morfológica e identificación de 12 aislamientos fungosos de semillas de Leucaena leucocephala cv. Perú Cultural and morphological characterization and identification of 12 fungal isolations in seeds of Leucaena leucocephala cv. Peru

    Directory of Open Access Journals (Sweden)

    J. C Lezcano

    2010-03-01

    Full Text Available La presente investigación tuvo como objetivo identificar los agentes fungosos asociados a las semillas de Leucaena leucocephala cv. Perú almacenadas al ambiente, a partir de la caracterización cultural y morfológica de 12 aislamientos puros; estos se obtuvieron de la siembra de las estructuras fúngicas (vegetativas y/o reproductivas, en placas Petri (9 cm de diámetro que contenían Agar Papa Dextrosa (APD y Agar Malta (AM. Las placas se incubaron durante 10 días a 25ºC, con alternancia de 8 h luz/16 h oscuridad o a oscuridad constante, según los requerimientos de cada organismo. Se identificaron siete agentes fungosos asociados a las semillas, los cuales se clasificaron teniendo en cuenta los caracteres culturales y morfológicos, y se corroboraron con las claves taxonómicas. Ello permitió agrupar: Penicillium expansum Link, Rhizopus stolonifer Ehrenb. ex Fr., Cladosporium sphaerospermum Penz., Chaetomium indicum Corda, Alternaria alternata (Fr Keissl., Pestalotia sp. y Trichoderma sp. Dichos caracteres constituyen una herramienta importante para la identificación de los hongos hasta el nivel de especie, por lo que se recomienda realizar nuevos estudios con los aislamientos #9 y #11 con vista a completar la identificación hasta la especie en el caso de los géneros Trichoderma y Pestalotia; así como identificar las especies fungosas del resto de los aislamientos.The objective of this study was to identify the fungal agents associated to seeds of Leucaena leucocephala cv. Peru stored under ambient conditions, from the cultural and morphological characterization of 12 pure isolations; they were obtained from planting the fungal structures (vegetative and/or reproductive, in Petri dishes (9 cm diameter which contained Potato Dextrose Agar (PDA and Malt Agar (MA. The dishes were incubated for 10 days at 25ºC, alternating 8 h light/16 h darkness or at constant darkness, according to the requirements of each organism. Seven fungal

  4. Content of heavy metals and naturally occurring radioactive material in Leucaena leucocaphala (Lam.) de wit

    International Nuclear Information System (INIS)

    Nurul Sareeza Azidin

    2012-01-01

    This research was done to determine the content of heavy metals in Leucaena leucocaphala (Lam.) de Wit (Petai Belalang) at 5 different areas. Those areas were in the middle of city, former mining area, industrial area, domestic waste disposal area, and on expressway roadside. Heavy metal poisoning can happen if the concentration is too high and will cause severe damage to human health. For instance, it may cause gene mutation, cancer and damage to the human body systems. This plant was selected for the study of heavy metals and radionuclide content in the soil. The reason of selecting this plant is because this plant can live in extreme conditions, and perhaps able to absorb those elements better than other legumes. The aim of this study was to determine the content of toxic heavy metals in leaves, stems and roots of Leucaena leucocaphala including the soil where it is grown. The second objective was determine NORM in the soil where the plant grew and the last objective was to determine the transfer factor of heavy metals by the plant. The content analysis of toxic heavy metals for example Cu, Zn, Cd, Hg and Pb were determined by using the Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Gamma ray spectrometry on the other hand, was used to determine K-40, Ra-226, U-238 and Th-232 in the soil where the plant was sampled. The activity concentration of Ra-226, K-40, U-238 and Th-232 determined were 7.47 ± 3.03 Bq/ kg - 256.92 ± 164.36 Bq/ kg, 95.55 ± 72.62 Bq/ kg - 435.60 ± 88.32 Bq/ kg, 21.83 ± 8.83 Bq/ kg - 165.28 ± 109.61 Bq/ kg dan 43.41 ± 7.06 Bq/ kg - 91.19 ± 11.13 Bq/ kg respectively. In general, the former mining area recorded the highest heavy metal concentration for Cu (28.20 ± 32.54 mg/ kg), Zn (114.67 ± 75,61 mg/ kg), Cd (0.31 ± 0.11 mg/ kg) and Pb (48.08 ± 33.60 mg/ kg). Whereas the highest concentration of As recorded was on the roadside (261.92 ± 132.64) and Hg (0.44 ± 0.36 mg/kg) in the middle of the city. (author)

  5. Characterization analysis for leaves of Leucaena leucocephala by using phytochemical screening assay

    Science.gov (United States)

    Zarina, Z.; Ghazali, C. M. R.; Sam, S. T.

    2017-09-01

    Leucaena Leucocephala (Lam.) de Wit (Petai Belalang) is a medium plant which belong in group of tropical breed that can survived in hot, dried and warm environment. In Malaysia, the plant is available abundantly. As there are still no commercial used, and no serious intention in finding the benefits of L. Leucocephala, this work come out with the idea to analyze the antioxidants contains in leaves of the plant by undergoes different extraction and chemical testing method. The phytochemical screening assay involved in this study are antioxidant activity by using free radical diphenylpicrylhydrazyl (DPPH) method, total phenolic content by using Folin-Ciocalteu method, total flavonoid content by using colorimetric assay with ascorbic acid and quercetin were used as reference standards while for phosphorus analysis, a molybdenum blue method or also known as ascorbic acid method was used. For antioxidant activity by using free radical diphenylpicrylhydrazyl (DPPH) method, higher concentration was recorded by extraction using methanol (dried sample) which is 8247.0 mg/L, for total phenolic content higher concentration was recorded by extraction using deionized water (dried sample) which is 4276.0 mg/L, for total flavonoid content by using colorimetric assay higher concentration was recorded by extraction using methanol (dried sample) which is 4439.0 mg/L, and for for phosphorus analysis higher concentration was recorded by extraction using methanol (dried sample) which is 71.057 mg/L.

  6. APPARENT DIGESTIBILITY OF RHODE ISLAND RED HEN DIETS CONTAINING Leucaena leucocephala AND Moringa oleifera LEAF MEALS

    Directory of Open Access Journals (Sweden)

    Khaled Abouelezz Fouad Mohammed

    2012-05-01

    Full Text Available This study consisted of two trials aimed to evaluate the dietary digestibility by Rhode Island Red (RIR hens' fed on different levels of Leucaena leucocephala (LLM or Moringa oleifera (MOLM. In each experiment, thirty six Rhode Island Red hens at 36 weeks of age were randomly divided into four groups each of nine birds which were allocated in individual cages. The four groups were corresponded to four dietary treatments containing 0 (control, 5, 10 and 15 % of LLM (Exp 1 or MOLM (Exp 2. All groups received smashed diets containing similar metabolizable energy and crude protein (16% CP and 2900 kcal ME/kg diet, as fed basis. The hens were fed the experimental diets for six weeks and during the last four days, feed intake was individually recorded every day and excreta was totally collected twice daily and weighed individually. Considerable amounts of CP were found in LLM (23.61% DM and MOLM (19.76% DM. The dietary treatments had no significant effect on the intake of dry matter (DM, organic matter (OM, gross energy (GE, crude protein (CP or neutral detergent fiber (NDF in both experiments, while the acid detergent fibers (ADF consumption increased linearly (P

  7. Selection and production of bacteria which detoxify mimosine: Leucaena leaves may be used as ruminant feed

    Energy Technology Data Exchange (ETDEWEB)

    Boehnel, H. [Institute of Tropical Animal Health, Georg-August-University Goettingen (Germany)], E-mail: hboehne@gwdg.de; Aung, A [Institute of Tropical Animal Health, Georg-August-University Goettingen (Germany); University of Veterinary Sciences, Yezin (Myanmar)

    2009-07-01

    Mimosaceae are shrubs or trees, which grow abundantly in tropical regions. Their leaves contain high value protein which cannot be used as feed due to the toxic substance mimosine and its metabolites in the digestive tract These alkaloids cause diseases in ruminants, mainly loss of hair/wool, and may lead to death in higher concentration. This is the reason why the nutritive value cannot be exploited reasonably in animal production. Experience has shown that there are some geographical regions where animals do not suffer by mimosine. It was found that they harbour ruminal bacteria, which degrade mimosine to non-toxic metabolites. In cooperation with other microbes in the digestive tract, the full dietetic value of Leucaena may be exploited. To date several bacteria were isolated and may be used as feed additive, e.g., S. jonesii. However, their production and storage is expensive and complicated. A practical method is to orally inoculate animals and use their rumen fluid directly as feed additive. This rumen culture or cultured anaerobic bacteria in the laboratory may suffer during transport and storage; hence, they need a cold chain until the target animal. Our institute has an outstanding experience to produce bacterial veterinary vaccines, probiotics, and biological fertilizers in tropical countries. Continuous culture in a bioreactor is the base for the success. It was the idea to use this technology for selection and production of mimosine degrading bacteria. The presentation will give a short theoretical background of - bacterial fermenter production - continuous culture - isolation of specific bacteria under selective environmental conditions - mass production in a bioreactor to be used in tropical areas - purification and concentration of the bacterial crop by rinsing and hollow fibre cross flow ultra filtration - stabilisation of the product in alginate beads - microbiota in the digestive tract. The practical work started with ruminal content of German

  8. Effect of combined inoculations of Rhizobium-Arbuscular Mycorrhiza in Leucaena leucocephala CV: Perú

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    Lázaro Jesús Ojeda Quintana

    2014-07-01

    Full Text Available The work was conducted at the Experimental Station of Soil and Fertilizer “Escambray” land of farmers and associated rural extension. The experimental design was randomized blocks, and treatments: Rizhobium loti V-4033 (Research Institute of Pastures and Forages, mycorrhizal fungi trainers, Rhizobium +mycorrhiza fungi trainers, nitrogen and total control. Inoculation with my corrhiza forming fungi was performed at 10 g/bag Certificate MicoFert Strain the Institute of Ecology and Systematics-3: Glomus spurcum, Topes de Collantes, 85 % colonization of rootlets, while the Rhizobium was inoculated in a dose of 50 g/kg of seed. Variant was nitrogen at 25 kg/ha of urea. The plots occupied 18m2 , with an area of 9 m2 evaluable. When the plants reached 7.5 months and an average height of 143.4 cm proceeded to make the Court of establishment and then four foliage cuts were made every 90 days. Each cut dry biomass yield, and phosphorus content was determined crude protein. The combined inoculation of Rhizobium and mycorrhiza fungi Makers increased the yield of dry biomass, and phosphorus content of crude protein. We conclude proposing the adoption of an alternative methodology using mycorrhiza formers Fungi and Rhizobium in Leucaena leucocephala as a way to increase the yield of dry biomass, decreasing mineral fertilization and contribute to environmental protection

  9. Evaluation of growth performance of broiler chicks fed with raw and processed leucaena eucociphala seed

    International Nuclear Information System (INIS)

    Minari, J.B.; Odutuga, A.A.

    2014-01-01

    Fourty-eight broiler chicks (day-old) were used in a 4 weeks feeding experiment to assess the growth response and the performance of broiler chicks fed raw Leucaena leucociphala seed meal (RLSM), roasted L. leucociphala seed meal (RoLSM) and steamed L. leucociphala seed meal (SLSM). The L. leucociphala seed, which serve as a source of protein were subjected to two treatments (roasting and steaming). Soybean based diet served as the control. There were four (4) experimental groups, each made up of four birds in three replicates allocated to the experimental diets. The results showed that the average final live weight, average weekly weight gain and average feed intake of birds fed with RoLSM performed better than birds fed with RLSM and SLSM. It was observed that the percentage organ to body weight of birds fed with the processed LSM were significantly (P < 0.05), higher as compared to the RLSM. A significant (P < 0.05) increase in the activity of aspartate transferase in the organs (heart, kidney and liver) of broilers fed with RoLSM was observed as compared to those fed with RLSM. The results showed a significant (P < 0.05), reduction in the activity of alanine transferase of organs of broilers chicks fed with processed L. leucociphala compared to the control. It is therefore, concluded that the processing techniques applied were able to improve the nutritional quality of L. leucociphala seed meal but relatively lower to the soybean based meal. (author)

  10. Phylogenetic and population genetic analyses of diploid Leucaena (Leguminosae; Mimosoideae) reveal cryptic species diversity and patterns of divergent allopatric speciation.

    Science.gov (United States)

    Govindarajulu, Rajanikanth; Hughes, Colin E; Bailey, C Donovan

    2011-12-01

    Leucaena comprises 17 diploid species, five tetraploid species, and a complex series of hybrids whose evolutionary histories have been influenced by human seed translocation, cultivation, and subsequent spontaneous hybridization. Here we investigated patterns of evolutionary divergence among diploid Leucaena through comprehensively sampled multilocus phylogenetic and population genetic approaches to address species delimitation, interspecific relationships, hybridization, and the predominant mode of speciation among diploids. Parsimony- and maximum-likelihood-based phylogenetic approaches were applied to 59 accessions sequenced for six SCAR-based nuclear loci, nrDNA ITS, and four cpDNA regions. Population genetic comparisons included 1215 AFLP loci representing 42 populations and 424 individuals. Phylogenetic results provided a well-resolved hypothesis of divergent species relationships, recovering previously recognized clades of diploids as well as newly resolved relationships. Phylogenetic and population genetic assessments identified two cryptic species that are consistent with geography and morphology. Findings from this study highlight the importance and utility of multilocus data in the recovery of complex evolutionary histories. The results are consistent with allopatric divergence representing the predominant mode of speciation among diploid Leucaena. These findings contrast with the potential hybrid origin of several tetraploid species and highlight the importance of human translocation of seed to the origin of these tetraploids. The recognition of one previously unrecognized species (L. cruziana) and the elevation of another taxon (L. collinsii subsp. zacapana) to specific status (L. zacapana) is consistent with a growing number of newly diagnosed species from neotropical seasonally dry forests, suggesting these communities harbor greater species diversity than previously recognized.

  11. Descomposición del follaje de Leucaena leucocephala cv. Cunningham asociada con Morus alba var. tigríada

    Directory of Open Access Journals (Sweden)

    F Ruz Súarez

    Full Text Available El presente estudio se diseñó en una parcela experimental donde se utiliza, desde hace diez años, el follaje de Leucaena leucocephala (leucaena como abono verde en el cultivo de Morus alba (morera, con el objetivo de determinar la velocidad de descomposición del follaje de la leguminosa y la relación de este proceso con algunos de los factores bióticos y abióticos presentes en el sistema. Para el estudio de la dinámica de la descomposición del follaje se utilizó el método de bolsas de hojarasca (litter bags. El follaje depositado en estas bolsas fue evaluado en seis momentos durante la etapa de estudio, que abarcó dos años. En cada fecha de recolección, a la hojarasca remanente de cada bolsa se le determinó la población de macrofauna mediante la separación manual, según la metodología del Programa de Investigación Internacional "Biología y Fertilidad del Suelo Tropical" (TSBF. La composición taxonómica de la macrofauna asociada al proceso de descomposición del follaje de leucaena estuvo constituida por cuatro Phylum, seis clases y siete órdenes. El 97% de los organismos eran detritívoros y el 3% depredadores. El tipo de bolsa no influyó en la descomposición del follaje y los factores climáticos desempeñaron un rol decisivo en este proceso.

  12. La productividad de cabras ramoneando Leucaena leucocephala en las áreas semi áridas del noroeste de Venezuela

    OpenAIRE

    Clavero, Tyrone; Razz, Rosa

    2009-01-01

    Un experimento de campo fue realizado en las áreas semiáridas del noroeste de Venezuela para evaluar tres tipos de dietas en cabras (pastoreo de gramíneas (pasto buffel); pastoreo de gramíneas + 0.3 kg/día de alimento comercial); pastoreo de gramíneas + ramoneo restrictivo de dos horas diarias en Leucaena leucocephala) sobre la producción y composición de la leche y la tasa de crecimiento. Los datos muestran diferencias significativas (P

  13. Combustion Characteristics of Torrefied Wood Samples of Pinus Carrebea and Leucaena Leucocephala Grown in Nigeria

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    Francis Akinyele FARUWA

    2016-12-01

    Full Text Available Torrefaction of selected wood samples of Pinus Carrebea and Leucaena Leucocephala were carried out at temperatures ranging from 200 to 300°C to improve the energy parameters of biomass and to determine the effect of torrefication temperature on the physical and combustion properties of wood selected from Pinus carrebea and Leuceanea leucocephala grown in Nigeria. In this process the biomass hemicellulose is degraded, maintaining its cellulose and lignin content. The samples were dried and heated to 225, 250, 275, and 300°C. Then the torrefied mass was subjected to basic property testing on proximate analysis and heating value was calculated in order to understand the differences between raw material and its torrefied products. Specifically, the wood blocks changed from light brown to black, stemming from the partial carbonization at the wood surface. When the temperature is 225°C, the color of the wood is between dark brown and once the torrefaction temperatures are 250 and 275°C, the colors of the wood become dark and darker respectively. The results of the proximate analysis also showed that increasing of torrefied temperature; volatile fraction was reduced while fixed carbon was increased with increase in temperature from 21.34 to 52.74 and 18.58 to 56.83 for Leucaena leucocephala and Pinus carreabeanus respectively at 225 to 300°C. The volatile content is decreased from 78.58% to 62.76% with increase in temperature. Ash content of were within 1.57-3.41% of torrefied wood. It could be observed that the High calorific value (HCV for pine ranged between 19.80 and 28.06MJ/Kg for the top, 19.93and 24.96MJ/kg for middle with 19.72and 25.96MJ/Kg for base. The values recorded for raw sample and at 275°C been the lowest and highest respectively. The High calorific value (HCV were found to be on the increase and nose dive at 300°C for the tree parts used in this research. The result revealed that for Leuceana the value increased from raw up to

  14. COMBUSTION CHARACTERISTICS OF TORREFIED WOOD SAMPLES OF PINUS CARREBEA AND LEUCAENA LEUCOCEPHALA GROWN IN NIGERIA

    Directory of Open Access Journals (Sweden)

    Joseph Adeola FUWAPE

    2016-12-01

    Full Text Available Torrefaction of selected wood samples of Pinus Carrebea and Leucaena Leucocephala were carried out at temperatures ranging from 200 to 300°C to improve the energy parameters of biomass and to determine the effect of torrefication temperature on the physical and combustion properties of wood selected from Pinus carrebea and Leuceanea leucocephala grown in Nigeria. In this process the biomass hemicellulose is degraded, maintaining its cellulose and lignin content. The samples were dried and heated to 225, 250, 275, and 300°C. Then the torrefied mass was subjected to basic property testing on proximate analysis and heating value was calculated in order to understand the differences between raw material and its torrefied products. Specifically, the wood blocks changed from light brown to black, stemming from the partial carbonization at the wood surface. When the temperature is 225°C, the color of the wood is between dark brown and once the torrefaction temperatures are 250 and 275°C, the colors of the wood become dark and darker respectively. The results of the proximate analysis also showed that increasing of torrefied temperature; volatile fraction was reduced while fixed carbon was increased with increase in temperature from 21.34 to 52.74 and 18.58 to 56.83 for Leucaena leucocephala and Pinus carreabeanus respectively at 225 to 300°C. The volatile content is decreased from 78.58% to 62.76% with increase in temperature. Ash content of were within 1.57-3.41% of torrefied wood. It could be observed that the High calorific value (HCV for pine ranged between 19.80 and 28.06MJ/Kg for the top, 19.93and 24.96MJ/kg for middle with 19.72and 25.96MJ/Kg for base. The values recorded for raw sample and at 275°C been the lowest and highest respectively. The High calorific value (HCV were found to be on the increase and nose dive at 300°C for the tree parts used in this research. The result revealed that for Leuceana the value increased from raw up to

  15. Leucaena allelopathy on weeds and soybean seed germination / Alelopatia de Leucena sobre soja e plantas invasoras

    Directory of Open Access Journals (Sweden)

    Raquel Leszczynski

    2009-07-01

    Full Text Available With the advance of agriculture, there was consequent rising in environment degradation. Therefore, It is interesting the use of plants which have in their chemical composition substances capable of helping in the control of weeds to minimize the use of pesticides. Hence, the objective of this paper was to evaluate solutions of leucena extract on weeds that are frequent on soybean crop. The treatments were leucena extracts at 0, 20, 40, 60, 80 and 100% at room temperature water and water heated to 80°C. The extracts were obtained by crushing 200g of leucena leaves and mixing them with one liter of distilled water with the help of a blender. Seeds were kept in B.O.D. at a temperature of 25°C, with a photoperiod of 12 hours of light. Evaluations were done on a daily basis, from the first to the last day of the experiment, varying according to the species. Results show a negative interference of the extracts, both hot and cold, in the root length of Ipomoea grandifolia, in the percentage of germination and in the root length of Arrowleaf sida and Hair beggarticks. There was no negative interference in the analyzed parameters for soybean seeds. Thus, the results indicate allelopatic potential of Leucaena leuucocephala as an alternative for the management of weeds without interfering on the development of the soybean crop.Com o desenvolvimento da agricultura surgiram os conseqüentes aumentos na degradação ambiental. Assim, estuda-se a utilização de plantas que possuam em sua composição química substâncias capazes de auxiliar no controle de plantas invasoras, amenizando o uso de agrotóxicos. Desta forma, o objetivo do trabalho foi avaliar soluções de extratos de leucena sobre plantas invasoras que freqüentemente ocorrem na cultura da soja. Foram utilizados como tratamentos os seguintes extratos de leucena nas proporções de: 0, 20, 40, 60, 80 e 100% com água à temperatura ambiente e com água aquecida à 80°C. Os extratos foram

  16. Leucaena lanceolata S. Watson ssp. lanceolata, ESPECIE FORESTAL CON POTENCIAL PARA SER INTRODUCIDA EN SISTEMAS SILVOPASTORILES

    Directory of Open Access Journals (Sweden)

    María L. Román-Miranda

    2013-01-01

    Full Text Available La utilización de especies forestales en los sistemas de producción agropecuaria contribuye a reducir la presión en los bosques naturales y se pueden incorporar en áreas no arboladas. El objetivo de este estudio fue evaluar la calidad nutritiva, germinación, desarrollo de plántula en vivero y diversidad de usos de Leucaena lanceolata S. Watson ssp. lanceolata. El material comestible y las semillas se colectaron en Tomatlán, Jalisco. Se realizaron análisis bromatológicos, pruebas de escarificación y evaluación de plántula en vivero sobre tres suelos con diferente pH. El experimento se analizó en un diseño completamente al azar con comparación de medias de Tukey (P ≤ 0.05. Además, se hicieron entrevistas a productores, una revisión bibliográfica y consulta de ejemplares en los herbarios para conocer los usos locales y potenciales de la especie. Los resultados indican alto contenido de materia seca (97.40 % y proteína cruda (29.05 %, mayor germinación en los tratamientos térmicos, mejor desarrollo de la plántula en el suelo ligeramente ácido (6.57 y la diversidad de usos incluye leña, forraje y madera, entre otros. Por el alto valor nutritivo y diversidad de usos en el medio rural, L. lanceolata representa una opción viable para utilizarse en sistemas silvopastoriles del trópico seco.

  17. COMPONENTES NUTRICIONALES Y ANTIOXIDANTES DE DOS ESPECIES DE GUAJE (Leucaena spp.: UN RECURSO ANCESTRAL SUBUTILIZADO

    Directory of Open Access Journals (Sweden)

    Nallely Román-Cortés

    2014-01-01

    Full Text Available El guaje ( Leucaena spp. es una planta de vaina y semillas comestibles, un recurso ancestral subutilizado por los pueblos mesoamericanos. El valor nutricional y nutracéutico del guaje se desconoce pese a su consumo vigente en las poblaciones rurales. El objetivo de este trabajo fue evaluar el contenido de minerales, compo - nentes nutricionales y antioxidantes para contribuir a la revalorización alimentaria del guaje rojo ( L. esculenta Benth. y verde ( L. leucocephala Lam.. Los contenidos de antocianinas, flavonoides, fenoles totales, taninos y actividad antioxidante se cuantificaron, así como la composición proximal y mineral. Las semillas de guaje rojo superaron a las de guaje verde en los contenidos de N, Mg, Mn, P y Zn; en contraste, las semillas de guaje verde presentaron niveles mayores de Na y de Fe. El contenido de fibra cruda fue 10.55 y 10.07 % en guaje rojo y verde, respectivamente, mientras que el de proteína fue 33.12 % en guaje rojo y 31.7 % en el verde. El alto contenido de compuestos fenólicos fue mayor en las semillas frescas de guaje rojo (1,088.70 mg equivalentes de ácido gálico [EAG]·100 g -1 p. f. que en las verdes (969.09 mg EAG·100 g -1 p. f., pero las últimas presen - taron mayor cantidad de taninos. La elevada actividad secuestradora de radicales libres (97.22 a 98.11 % obtenida por el método ABTS ( á cido 2,2’-azino-bis(3-etilben-zotiazolin-6-sulfónico, puede estar asociada al contenido de compuestos fenólicos. En conclusión, las semillas de guaje podrían considerarse un alimento funcional, principalmente las de guaje rojo debido a su calidad nutricional y mayor actividad antioxidante.

  18. Supplementation of native grass hay with cowpea (Vigna unguiculata hay, wilted leucaena (Leucaena leucocephala forage, wilted tagasaste (Chamaecytisus palmensis forage or a wheat middling for young Friesian x Zebu (Boran crossbred steers

    Directory of Open Access Journals (Sweden)

    Tuomo Varvikko

    1992-03-01

    Full Text Available A 100-day experiment of a randomized block design was conducted with forty Friesian x Zebu (Boran crossbred growing steers to compare the value of wheat middling, an agro-industrial by-product (diet W, cowpea (Vigna unguiculata hay (diet C, and wilted forages of leucaena (Leucaena leucocephala, diet L and tagasaste (Chamaecytisus palmensis, diet T as cattle feed. These feeds were fed daily at a level of 1.5 kg (on an air dry basis to supplement the basal diet (diet H of native hay. A mineral supplement containing 50 g bone meal and 10 g common salt was also given daily. The steers were group-fed, but during the last two weeks at the end of the experiment the animals were housed individually in feeding pens to estimate the feed intake and apparent digestibilities of the diets. The animals were weighed at the beginning of the experiment, thereafter every two weeks, and finally at the end of the experiment. The animals consumed all the offered supplements, except for tagasaste forage, of which one third remained unconsumed. The mean daily total dry matter intake during the individual feeding period ranged from 4.0 to 5.0 kg between the diets (P

  19. CRECIMIENTO, RELACIONES HÍDRICAS E INTERCAMBIO GASEOSO DE PLANTAS JÓVENES DE ILAMA (Annona diversifolia Saff. EN CONDICIONES DE ESTRÉS HÍDRICO

    Directory of Open Access Journals (Sweden)

    M. A. Otero-Sánchez

    2005-01-01

    Full Text Available Se estudiaron algunas características morfológicas y fisiológicas de Annona diversifolia Saff. con relación a condiciones de disponibilidad de agua del suelo. El nivel de humedad del suelo afectó el área foliar y la distribución de materia seca. El potencial hídrico de la hoja disminuyó a medida que se redujo el potencial hídrico del suelo, registrándose valores de -1.26, -1.51, 1.73, 1.76 y 2.36 MPa, en el testigo, sequía 1, sequía 2, sequía 3 y tratamiento sin riego, respectivamente. La tasa de intercambio gaseoso también fue reducida significativamente en los tres niveles de humedad del suelo. La concentración de prolina registró una correlación negativa con potencial osmótico, sin embargo, el análisis de regresión no presentó ninguna tendencia definida. Después de 10 días del riego de recuperación, se registró el restablecimiento casi completo de la tasa de intercambio gaseoso en los tratamientos de estrés moderado.

  20. Growth performance, nutrient utilization, and feed efficiency in broilers fed Tithonia diversifolia leaf meal as substitute of conventional feed ingredients in Mizoram.

    Science.gov (United States)

    Buragohain, Rajat

    2016-05-01

    The study was for assessment of growth performance, nutrient utilization, and feed efficiency in broilers fed rations with varying levels of Tithonia diversifolia leaf meal (TDLM) as a substitute of conventional feed ingredients in Mizoram. A total of 180, 1-day-old broiler chicks were randomly divided into six homogeneous groups and fed rations incorporated with TDLM (TDLM at 0% [TDLM-0], 2% [TDLM-2], 4% [TDLM-4], 6% [TDLM-6], 8% [TDLM-8], and 10% [TDLM-10] level as substitute of conventional feed ingredients) for 6 weeks. The chicks were reared in battery brooders for the first 2 weeks, and thereafter, in well-ventilated deep litter house following standard management protocols. Feed and drinking water were provided ad libitum to all the groups throughout the experiment. The daily feed intake and weekly body weight gain were recorded, and a metabolic trial for 3 days was conducted at the end of the 6(th) week. Feed consumption decreased for inclusion of TDLM but without any significant differences, except during the 3(rd) week where it reduced significantly (pbroilers reared under deep litter system of management in Mizoram.

  1. Biomass production of Prosopis species (mesquite), leucaena, and other leguminous trees grown under heat/drought stress

    Energy Technology Data Exchange (ETDEWEB)

    Felker, P.; Cannell, G.H.; Clark, P.R.; Osborn, J.F.; Nash, P.

    1983-01-01

    Leguminous trees were examined for use on hot/arid lands in field trials in the Califronia Imperial Valley where July daily maximum temperatures are 42/sup 0/C (108/sup 0/F). Two field trials were carried out to rank 55 accessions in biomass per tree and to evaluate biomass production per unit area with four of the more productive accessions identified in earlier trials. The trial with 55 accessions compared Prosopis (mesquite) to widely recommended species for arid lands such as Leucaena leucocephala (K-8), Parkinsonia aculeata, and Prosopis tamarugo and to other drought adapted tree legume species of California/Arizona deserts such as Cercidium fluoridium and Olneya tesota. Prosopis selections were identified that had greater productivity than either Leucaena leucocephala (K-8) or Parkinsonia aculeata. The mean ovendry biomass per accession ranged from 0.2 kg/tree for Prosopis tamarugo to 29 kg/tree for P. alba (0166) when measured 2 years from germination in the greenhouse. Clones were obtained from trees in this trial which had 45-56 kg/tree (ovendry) in two seasons. The plots designed to measure biomass production per unit area were on a 1.5 m spacing and had productivities of 7, 11.2, 14.3, and 14.5 ovendry T ha/sup -1/ yr/sup -1/ for P. glandulosa var torreyana (0001), P. alba (0163), P. chilensis (0009), and P. alba (0039), respectively, when measured 2 years from germination in the greenhouse.

  2. Biomass production of Prosopis species (mesquite), Leucaena, and other leguminous trees grown under heat/drought stress

    Energy Technology Data Exchange (ETDEWEB)

    Felker, P.; Cannell, G.H.; Clark, P.R.; Osborn, J.F.; Nash, P.

    1983-09-01

    Leguminous trees were examined for use of hot/arid lands in field trials in the California Imperial Valley where July daily maximum temperatures are 42 degrees C (108 degrees F). Two field trials were carried out to rank 55 accessions in biomass per tree and to evaluate biomass production per unit area with four of the more productive accessions identified in earlier trials. The trial with 55 accessions compared Prosopis (mesquite) to widely recommended species for arid lands such as Leucaena leucocephala (K-8), Parkinsonia aculeata, and Prosopis tamarugo and to other drought adapted tree legume species of California/Arizona deserts such as Cercidium floridium and Olneya tesota. Prosopis selections were identified that had greater productivity than either Leucaena leucocephala (K-8) or Parkinsonia aculeata. The mean oven-dry biomass per accession ranged from 0.2 kg/tree for Prosospis tamarugo to 29 kg/tree for P. alba (0166) when measured 2 years from germination in the greenhouse. Clones were obtained from trees in this trial which had 45-56 kg/tree (oven-dry) in two seasons. The plots designed to measure biomass production per unit area were on a 1.5 m spacing and had productivities of 7, 11.2, 14.3, and 14.5 oven-dry T ha-1 yr-1 for P. glandulosa var torreyana (0001), P. alba (0163), P. chilensis (0009), and P. alba(0039), respectively, when measured 2 years from germination in the greenhouse. 30 references

  3. Ithome lassula Hodges (Lepidoptera: Cosmopterigidae, una nueva especie para Cuba asociada a Leucaena leucocephala (Lam. de Wit

    Directory of Open Access Journals (Sweden)

    O Alonso

    Full Text Available El nuevo informe para Cuba de Ithome lassula Hodges (Lepidoptera: Cosmopterigidae, especie asociada a la planta forrajera Leucaena leucocephala, se confirmó con la identificación de los adultos, mediante la utilización de una clave basada en la maculación de las escamas en la zona de la cabeza del insecto. Los adultos emergieron en el laboratorio, de las larvas colectadas en las inflorescencias; estas procedían de los diferentes sistemas ganaderos que se muestrearon en las provincias Matanzas y Mayabeque, los cuales estaban compuestos indistintamente por las cuatro variedades comerciales de la leguminosa y por gramíneas pratenses. Por otra parte, la detección de las lesiones que causaron las larvas de este cosmopterígido en las inflorescencias, y su repercusión en la producción de semillas de leucaena, constituyó el elemento más importante que se debía considerar para valorar su potencialidad como plaga y al estimar las posibles pérdidas en los sistemas ganaderos evaluados.

  4. Study on Leucaena leocochepala seed during fermentation : sensory characteristic and changes on anti nutritional compounds and mimosine level

    Science.gov (United States)

    Nursiwi, A.; Ishartani, D.; Sari, AM; Nisyah, K.

    2018-01-01

    Lamtoro (Leucaena leucocephala) seed is one of the leguminosae which have high level of protein but it contains toxic compound such as mimosine and some anti nutritional compounds such as phitic acid and tannin. The objectives of the research was to investigate the sensory characteristic and the changes onanti nutritional compounds and mimosine level in Leucaena leucochepala seed during fermentation. Lamtoro tempeh processing was carried out by boiling the seed, crushing to separate the hull, soaking, boiling, and fermentation. The best concentration inoculum in lamtoro tempeh processing was determined by hedonic test. Fermentation was carried out in 36 hours and every 6 hours mimosine, tannin, and phitic acid content was analyzed. From hedonic test, inoculum concentration of 1% was used in lamtoro tempeh processing. During 36 hours fermentation, phytic acid content and mimosine content was decreased significantly, from 0.0558 % to 0.0453 % and from 0.00393 % to 0.00173 % respectively. Whereas tannin content was increased signifacantly, from 0.0822 % to 0.00173 %.

  5. Effect of Leucaena and Sesbania supplementation on body growth and scrotal circumference of Ethiopian highland sheep and goats fed teff straw basal diet

    NARCIS (Netherlands)

    Kaitho, R.J.; Tegegne, A.; Umunna, N.N.; Nsahlai, I.V.; Tamminga, S.; Bruchem, J. van; Arts, J.M.

    1998-01-01

    The long term effect of supplementation of Leucaena pallida and Sesbania sesban on growth and reproduction performance was determined on 30 male Ethiopian highland sheep and 25 East African goats. Unchopped teff straw (Eragrostis tef) was given ad libitum and supplemented with either wheat bran (150

  6. Efecto de la altura y frecuencia de corte sobre la producción de materia seca y proteína cruda de Tithonia diversifolia (Hemsl) A. Gray

    OpenAIRE

    Lugo Soto, Maria; Molina, Francelina; González, Ignacio; González, Johan; Sánchez, Eudomar

    2012-01-01

    Para evaluar el efecto de la altura y frecuencia de corte en la producción de materia seca y proteína cruda de Tithonia diversifolia, se realizó un experimento en el Campo Experimental Ciudad Bolivia del Instituto Nacional de Investigaciones Agrícolas (INIA), ubicado en Ciudad Bolivia, estado Barinas a 08º 22` N y 70º 36´ O, y una elevación de 186 m.s.n.m. Se utilizó un diseño experimental de bloques al azar con tres repeticiones en un arreglo factorial de los tratamientos en parcelas dividid...

  7. First report of Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Lecanodiaspididae) and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae) in Brazil.

    Science.gov (United States)

    Marsaro Júnior, A L; Peronti, A L B G; Costa, V A; Morais, E G F; Pereira, P R V S

    2016-02-01

    Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Coccoidea: Lecanodiaspididae) and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae) are reported for the first time in Brazil. Specimens of this scale insect were collected on branches and stems of Acacia mangium Willd., Leucaena leucocephala (Lam.) de Wit (Fabaceae), Morus nigra L. (Moraceae), Citrus reticulata Blanco (Rutaceae), Tectona grandis L. f. (Verbenaceae), Anacardium occidentale (Anacardiaceae), Annona squamosa L. and Xylopia aromatica (Lam.) Mart. (Annonaceae), in three municipalities of the Roraima state. All plants here mentioned are recorded for the first time as a host for L. dendrobii. Morphological characters of L. dendrobii and symptoms presented by the host plants infested by this pest are included in this work.

  8. Pemanfaatan Tithonia Diversifolia pada Tanah Sawah yang Dipupuk P Secara Starter terhadap Produksi serta Serapan Hara N, P, dan K Tanaman Padi

    Directory of Open Access Journals (Sweden)

    Gusnidar

    2008-09-01

    Full Text Available The research of Tithonia diversifolia utilization in the paddy soil that was fertilized P-starter on yield and N, P, K uptake on rice crop, has been conducted from August to December 2005. The objective of this research was to determine an interaction between P-starter levels, and tithonia levels on production and N, P, K element uptake of rice crop. The research was pot experiment in the green house in factorial design 4 x 4. First factor was 4 levels of tithonia with reduce dosage fertilizer applied (T0 = 0 t ha-1 of tithonia + 200 kg ha-1 of urea + 75 kg ha-1 of KCl ; T1= 2,5 t ha-1 of tithonia+ 150 kg ha-1 of urea , without KCl; T2 = 5,0 ton ha-1 of tithonia+ 100 kg ha-1 of urea , without KCl; dan T3 = 7,5 t ha-1 of tithonia + 50 kg ha-1 of urea, without KCl. The second factor was 4 levels of P-starter (0, 2, 4, and 6 kg P ha-1. The result showed that combination of T3 treatment and 2-4 kg P-starter ha-1 increased the grain yield 20,51-21,08 g pot-1 (18,65-19,21 %. Effect of T3 treatment was not significantly differences with T2 treatment on the grain yield. The best interaction was T3 treatment on N and K uptake (0,84 g pot-1 for N, dan 0,82 g pot-1 for K.

  9. [Phagodeterrent activity of the plants Tithonia diversifolia and Montanoa hibiscifolia (Asteraceae) on adults of the pest insect Bemisia tabaci (Homoptera: Aleyrodidae)].

    Science.gov (United States)

    Bagnarello, Gina; Hilje, Luko; Bagnarello, Vanessa; Cartín, Victor; Calvo, Marco

    2009-12-01

    Bemisia tabaci (Gennadius) is a polyphagous, cosmopolitan and worldwide relevant pest, mainly acting as a virus vector on many crops. A sound preventive approach to deal with it would be the application of repellent or deterrent substances hopefully present in tropical plants, which in turn may contribute to take advantage of the remarkable rich Mesoamerican biodiversity. Therefore, extracts of two wild plants belonging to family Asteraceae, titonia (Tithonia diversifolia) and "tora" (Montanoa hibiscifolia), were tested for phagodeterrence to B. tabaci adults. The crude leaf extract of each one, as well as four fractions thereof (hexane, dichlorometane, ethyl acetate, and methanol) were tested under greenhouse conditions; in addition, the extracts were submitted to a phytochemical screening to determine possible metabolites causing phagodeterrence. Both restricted-choice and unrestricted-choice experiments were conducted. In the former ones, each fraction was tested at four doses (0.1, 0.5, 1.0 and 1.5% v/v), which were compared with four control treatments: distilled water, endosulfan, an agricultural oil (Aceite Agricola 81 SC), and the emulsifier Citowett. Tomato plants were sprayed and placed inside sleeve cages, where 50 B. tabaci adults were released. The criterion to appraise phagodeterrence was the number of landed adults on plants at 48h. For the unrestricted-choice experiments, only the two highest doses (1.0 and 1.5%) of the crude extracts of each species were tested, and compared to distilled water and the agricultural oil. The titonia and "tora" crude extracts caused phagodeterrence, and for both plant species the methanol fraction stood out. Results suggest that metabolites causing phagodeterrence are several sesquiterpenic lactones, polyphenolic compounds (flavonoids and tannins) and saponins.

  10. Diversidad zoológica asociada a un silvopastoreo leucaena-guinea con diferentes edades de establecimiento Zoological diversity associated to a silvopastural system leucaena-guinea grass with different establishment times

    Directory of Open Access Journals (Sweden)

    Jatnel Alonso Lazo

    2007-12-01

    Full Text Available El objetivo de este trabajo fue evaluar la diversidad zoológica asociada a un silvopastoreo con leucaena-guinea, por medio de la caracterización de la composición y estructura de las aves, insectos y la macrofauna del suelo, en cuatro edades de establecimiento (3, 4, 5 y 6 años de explotación. Con las especies registradas en cada uno de estos grupos zoológicos, se calcularon los índices ecológicos: número de individuos, riqueza, diversidad y abundancia de especies, en diferentes edades del sistema. En todos los grupos, se apreció el aumento significativo en la riqueza de especies y en el índice de diversidad biológica de Shannon, en la medida que se desarrolló el sistema. Se observó incremento en la abundancia de insectos biorreguladores y, en relación con las aves, el horario de muestreo no mostró interacción con los distintos años de siembra. La macrofauna se incrementó, observándose dominancia de anélidos al 6º y 7º año de explotación, caracterizado por Polyferetrina elongata y Oligochaeta elegans. El desarrollo del silvopastoreo leucaena-guinea logra sistemas productivos pecuarios que aumentan la producción de biomasa y de otros componentes biológicos y contribuir para crear un sistema sostenible y compatible con el ambiente.The aim of this work was to evaluate the associated zoological diversity of a silvopastural system leucaena-guinea grass, by characterizing the composition and structures of the birds, insects and the macrofauna of the soil, in four establishment times of the silvopastural systems (3, 4, 5 and 6 years of exploitation. For the species recorded in each zoological group, the following ecological indices were determined: number of individuals, richness, diversity and abundance of species, in each establishment times of the system. A significant increase, in all the zoological groups, was observed for the richness of species and for the index of biological diversity of Shannon, as the system

  11. Comportamiento de la disponibilidad de biomasa y la composición química en 23 accesiones de Leucaena spp. Performance of biomass availability and chemical composition of 23 Leucaena spp. accessions

    Directory of Open Access Journals (Sweden)

    Hilda B Wencomo

    2012-03-01

    Full Text Available Se realizó un estudio con 23 accesiones de Leucaena spp. en un área que tenía seis años de sembrada. El objetivo fue determinar, en las plantas establecidas, la biomasa total, la biomasa comestible, la biomasa leñosa, el grosor del tallo y el número de ramas de cada accesión, y su composición bromatológica en condiciones de pastoreo simulado. La investigación se realizó en la EEPF "Indio Hatuey" durante dos años, en un suelo Ferralítico Rojo lixiviado, húmico nodular ferruginoso hidratado. Se utilizaron parcelas sencillas de 3 m x 6 m. En la biomasa comestible y sus componentes (hojas y tallos tiernos existieron diferencias significativas (PA study was conducted with 23 Leucaena spp. accessions in an area which had been planted six years before. The objective was to determine, in the established plants, total biomass, edible biomass, ligneous biomass, stem diameter and number of branches in each accession, and its bromatological composition under simulated grazing conditions. The research was conducted at the EEPF "Indio Hatuey" during two years, on a hydrated ferruginous nodular humic lixiviated Ferralitic Red soil. Simple 3 m x 6 m plots were used. In the edible biomass and its components (leaves and fresh stems, there were significant differences (P<0,05 between the seasons of the two years. It could be observed that production was higher in the rainy season than in the dry season, although there were no differences among the mean biomass production of the accessions; in the case of total biomass no significant differences were found between seasons. To continue the studies is recommended, to determine, in the long term, the effect of the evaluated indicators on the availability and persistence of the tree.

  12. Growth and N-uptake in sorghum plants manured with different amounts of Leucaena Leucocephala shoots as affected by time of application

    International Nuclear Information System (INIS)

    Kurdali, F.; Al-Shamma'a, M.

    2007-01-01

    A pot experiment was conducted throughout two successive years to determine the impact of adding four rates of nitrogen (0, 60, 120 and 180 kg N/ha) in the form of Leucaena leucocephala (lam.) de Wit green manure as affected by different time (T0, T15 and T30) of application (just before sowing, 15 and 30 days before sowing, respectively) on the performance of sorghum (Sorghum bicolor L.) using the indirect N-15 isotopic dilution technique. Results showed that leucaena leaves, used as a green manure, significantly increased dry matter production and N yield of sorghum. The effect was more pronounced in panicles than shoots. In the first year, N recoveries in sorghum of the total N contained in leucaena green manure ranged between 17-24% in T0, 14-24% in T15 and 15-19% in T30. The highest N recovery value was obtained in lowest rate of n treatment (N60). Moreover, soil incorporated with leucaena green manure one month before planting enhanced both soil and mineral N fertilizer in sorghum plants. In the second year, total N uptake in sorghum (eg., panicles and the entire plant) increased with increasing amounts of N added as a leucaena green manure. N recoveries in sorghum ranged between 26-47% in T0, about 24% in T15 and 23-325 in T30 of the total N contained in leucaena green manure (LGM). As shown in the first year, the highest value of N recovery was obtained in lowest rate of N treatment. The beneficial effects of leucaena green manure on dry matter and N yield in sorghum was attributed not only to the additional N availability to the plant, but also to effects on the enhancement of soil N uptake, particularly when the incorporation was made before a sufficient time from sowing. The beneficial effect of green manuring with leucaena leaves at T15 and T30 was mainly resulted from enhancement of N uptake from this added material, as well as from soil and fertilizer N. Whereas, it was only attributed to N uptake from green manure in the T0 treatment. Incorporation

  13. Growth and N-uptake in sorghum plants manured with different amounts of Leucaena Leucocephala shoots as affected by time of application

    International Nuclear Information System (INIS)

    Kurdali, F.; Al-Shamma'a, M.

    2006-08-01

    A pot experiment was conducted throughout two successive years to determine the impact of adding four rates of nitrogen (0, 60, 120 and 180 kg N/ha) in the form of Leucaena leucocephala (lam.) de Wit green manure as affected by different time (T0, T15 and T30) of application (just before sowing, 15 and 30 days before sowing, respectively) on the performance of sorghum (Sorghum bicolor L.) using the indirect N-15 isotopic dilution technique. Results showed that leucaena leaves, used as a green manure, significantly increased dry matter production and N yield of sorghum. The effect was more pronounced in panicles than shoots. In the first year, N recoveries in sorghum of the total N contained in leucaena green manure ranged between 17-24% in T0, 14-24% in T15 and 15-19% in T30. The highest N recovery value was obtained in lowest rate of n treatment (N 60 ). Moreover, soil incorporated with leucaena green manure one month before planting enhanced both soil and mineral N fertilizer in sorghum plants. In the second year, total N uptake in sorghum (eg., panicles and the entire plant) increased with increasing amounts of N added as a leucaena green manure. N recoveries in sorghum ranged between 26-47% in T0, about 24% in T15 and 23-325 in T30 of the total N contained in leucaena green manure (LGM). As shown in the first year, the highest value of N recovery was obtained in lowest rate of N treatment. The beneficial effects of leucaena green manure on dry matter and N yield in sorghum was attributed not only to the additional N availability to the plant, but also to effects on the enhancement of soil N uptake, particularly when the incorporation was made before a sufficient time from sowing. The beneficial effect of green manuring with leucaena leaves at T15 and T30 was mainly resulted from enhancement of N uptake from this added material, as well as from soil and fertilizer N; whereas, it was only attributed to N uptake from green manure in the T0 treatment. Incorporation

  14. Considerations about Leucaena leucocephala cv. X: A new forage choice for a livestock ecosystem with acid and infertile soils

    OpenAIRE

    Pérez, A; Wencomo, Hilda; Navarro, Marlen; Iglesias, J.M; Soca, Mildrey; Cepero, L; Canchila, E.R

    2008-01-01

    El presente estudio se realizó a partir de una búsqueda de información por diversas páginas Web, revistas de impacto, otras de carácter nacional e internacional, así como las más diversas fuentes de información, relacionada con la capacidad de adaptación del género Leucaena a los diferentes tipos de suelo, con énfasis en la acidez y el grado de encharcamiento. La revisión proporcionó las limitantes de adaptación del género y de la especie L. leucocephala a dichas condiciones. El descubrimient...

  15. Chemical elements in Leucaena leucocephala leaves of riparian zones of the municipality of Piracicaba, São Paulo, Brazil

    International Nuclear Information System (INIS)

    França, Elvis J. de; Fernandes, Elisabete A.N.; Lira, Marcelo G.; Ferreira, Fabiano S.; Cavalca, Isabel P.O.; Rodrigues, Vanessa S.; Camilli, Leandro

    2017-01-01

    The species Leucaena leucocephala can be found in several riparian ecosystems, acting as one of the main invasive and harmful species for the forest restoration of the permanent preservation areas. It has also been studied for the phytoremediation of some chemical elements, due to their potential accumulation of chemical substances. Therefore, the present study aimed to evaluate the accumulation of chemical elements in leaves of Leucaena leucocephala trees by Instrumental Neutronic Activation Analysis - INAA. Samples of leaves were collected in eight samples from riverside areas of the Piracicaba Municipality, São Paulo, Brazil, during the dry and rainy season. After collection, washing with water and drying in a forced circulation oven, the samples were comminuted and encapsulated in polyethylene capsules and subjected to a thermal neutron flux of 10 13 cm -2 s -1 for 8 hours in the Nuclear Research Reactor IEA-R1 of IPEN / CNEN. Thermal neutron flux monitoring was performed by Ni-Cr alloy fragments with known concentrations of the chemical monitors elements. After measurements of radioactivity induced by HPGe detectors, the concentrations of the chemical elements were calculated by the k0 method using the Quantu computer program. Certified reference materials were also analyzed for quality assurance of the analytical procedure. The results indicated high concentrations of La and lanthanoids (Ce, Eu, Sm, Tb and Yb) in leaves of L. leucocephala in both periods analyzed. It was possible to observe a decrease in the concentrations of the chemical elements in the rainy season, probably associated to the washing of the geological material deposited on the leaves of the trees by the rains, except for K, Mo, Rb, Sb and Zn, whose values remained or increased in the leaves collected during the rainy season. Considering the constant leaf production, L. leucocephala can greatly affect the cycling of chemical elements due to its accumulation capacity demonstrated by this

  16. Performance of Dairy Goats Fed Rhodes Grass Hay and Supplemented with Leucaena or Gliricidia-Based Concentrates

    International Nuclear Information System (INIS)

    Ondiek, J.O.; Tuitoek, J.K.; Abdulrazak, S.A.

    1999-01-01

    An experiment was conducted to evaluate the performance of dairy goats supplemented with fodder based diets. Six lactating does of Saanen, Toggenburg and Saanen X Toggenburg breed types weighing 46 + or - 7.1 at late stage of lactation were allocated to three dietary treatments in a double 3*3 Latin square design to examine the value of leucaena and gliricidia forages as nitrogen sources in supplementing Rhodes grass hay. Three supplements: Leucaena-based (L), gliricidia-based (G) or commercial concentrate (C) were used. Voluntary feed intake, milk yield, milk composition and live weight changes were measured. Total dry matter intake (DMI) was significantly higher in group G than C (1385 vs 1331 g day -1 ). Hay DMI was higher in G (835 g d -1 than L or C (789, 782 g day -1 , respectively). Diets had no effect on milk yield, crude protein or butterfat content. Total solid was higher in L than C while that of G was intermediate (15.7, 14.7, 13.7%). Weight changes were least affected although resulted in positive gain (11 g day -1 ) while the others lost (-13,-19 g day -, for L and C respectively). In the digestibility trial 6 female goats weighing 22.3 kg (sd 1.1), were allocated to the three treatments in a completely randomised block design where each goat received a different supplement in each of two periods. Thee were no differences in nutrient digestibility except for DM, which was in C compared to the other treatments (615, 622, 720 g-kg for L, G and C, respectively). It si concluded that fodder based supplement compared well with the commercial concentrate in supporting milk yield and body weight gain of dairy goats. Therefore, locally available tree fodders can be incorporated in supplement diets and utilized without any detrimental effects to substitute the expensive concentrates to improve the performance of dairy goats

  17. Growth and Nitrogen Uptake in Sorghum Plants Manured with Leucaena Leucocaphala Leaves as Affected by Nitrogen Rate and Time of Application

    International Nuclear Information System (INIS)

    Kurd Ali, F.; Al-Shammaa, M.

    2011-01-01

    A pot experiment was conducted to determine the effect of four rates of nitrogen (N) in the form of leucaena leaves and the time of application on the performance of sorghum plants using the 15 N isotopic dilution technique. Results showed that leucaena green manure (LGM) increased dry matter and N yield of sorghum. Nitrogen recoveries of LGM ranged between 23 and 47%. An additional beneficial effect of LGM was attributed to the enhancement of soil N uptake. The best timing of LGM incorporation for obtaining more N derived from LGM, less soil N uptake, and greater dry matter and N in sorghum leaves seemed to be at planting. However, the appropriate timing and rate of LGM to obtain greater dry matter and N yield in panicles, as well as in the whole plant of sorghum, appeared to be at 30 days before planting, particularly a rate of 120 kg N ha - 1. (author)

  18. Productive performance and urinary excretion of mimosine metabolites by hair sheep grazing in a silvopastoral system with high densities of Leucaena leucocephala.

    Science.gov (United States)

    Barros-Rodríguez, Marcos; Solorio-Sánchez, Javier; Ku-Vera, Juan; Ayala-Burgos, Armín; Sandoval-Castro, Carlos; Solís-Pérez, Georgina

    2012-12-01

    The aim of this study was to evaluate daily weight gain (DWG), total dry matter (DM) intake, rumen degradability of forage, and urinary excretion of mimosine metabolites by hair sheep in a silvopastoral system with high densities of Leucaena leucocephala. A completely randomized design was carried out with two treatments: treatment 1 (T1) silvopastoral system with leucaena at a density of 35,000 plants/ha and treatment 2 (T2), leucaena at a density of 55,000 plants/ha. Leucaena was associated with tropical grasses Panicum maximum and Cynodon nlemfluensis. Twenty-four male Pelibuey lambs of 23.2 ± 3.4 kg live weight (LW) were used (12 lambs per treatment). Results showed differences (P < 0.05) in DWG of T1 (106.41 ± 11.66 g(-1) sheep(-1)) with respect to that of T2 (81.33 ± 11.81 g(-1) sheep). Voluntary intake was higher in lambs from T1 (83.81 ± 04.07 g DM/kg LW(0.75)) with respect to that from T2 (71.67 ± 8.12 g DM/kg LW(0.75)). There was a difference in color of urine between sheep of T1 and T2, the latter giving positive results for the presence of metabolites derived from mimosine (3-4 dihydroxypyridine and 2-3 dihydroxy pyridone). Rumen degradability of DM of L. leucocephala was higher (P < 0.05) compared to that of P. maximum and C. nlemfluensis (72.94 ± 0.40 vs. 67.06 ± 1.50 and 63.25 ± 1.51 %, respectively). It is concluded that grazing at high densities of L. leucocephala affects daily weight gain of hair sheep, possibly due to ingestion of high amounts of mimosine which may exert an adverse effect on voluntary intake.

  19. Efecto del agua caliente en la germinación de las semillas de Leucaena leucocephala cv. Perú Effect of hot water on the germination of seeds from Leucaena leucocephala cv. Peru

    Directory of Open Access Journals (Sweden)

    Yolanda González

    2008-03-01

    Full Text Available En un diseño de clasificación simple se estudió el efecto del tratamiento pregerminativo con agua a 80ºC por dos minutos, en la germinación de las semillas de Leucaena leucocephala cv. Perú almacenadas al frío y al ambiente, durante siete años. Se demostró la presencia de dormancia moderada, con un porcentaje de germinación de 67,3% en la semilla recién cosechada, que fue inferior a la potencialidad germinativa mostrada a través de su alta viabilidad (98,2%. En condiciones controladas y al ambiente la germinación presentó diferencias altamente significativas para los meses de almacenamiento (PIn a simple classification design the effect of the pregerminative treatment with water at 80ºC for two minutes, on the germination of seeds from Leucaena leucocephala cv. Peru stored under cold-storage room and ambient conditions, for seven years, was studied. The presence of moderate dormancy was proven, with a germination percentage of 67,3% in the newly harvested seed, which was lower than the germination potential shown through their high viability (98,2%. Under controlled and ambient conditions germination showed highly significant differences for the storage months (P<0,001 and the highest values without applying pregerminative treatment were achieved at 12, 42 and 48 months (77,6; 71,8 and 75,7% and at 18 months (81,8%, respectively. The application of water at 80ºC for two minutes produced significant increases (P<0,001 in germination with regards to the untreated seed and under controlled conditions it was higher at 12, 24 and 48 months (98,6; 99,6 and 98,5% and under ambient conditions at 6, 12 and 18 months (96,2; 97,7 and 97,7%. It is concluded that the newly harvested seeds from L. leucocephala cv. Peru show around 30% dormancy and can increase their germination by applying water to them at 80ºC for two minutes.

  20. Disponibilidad de biomasa y hábitos alimenticios de ovinos en un sistema silvopastoril con Leucaena leucocephala, Hibiscus rosa-sinensis Y Cynodon nlemfuensis

    Directory of Open Access Journals (Sweden)

    Bernardino Candelaria-Martínez

    2017-01-01

    Full Text Available Se evaluó el efecto del pastoreo a los 50, 60 y 70 días en época de lluvias y seca con nortes que son los frentes fríos, que corresponden de octubre a enero junto con la disponibilidad, calidad de biomasa y hábitos alimenticios de ovinos en un sistema silvopastoril de pasto estrella (Cynodon nlemfuensis, tulipán (Hibiscus rosasinensis y leucaena (Leucaena leucocephala. Para cada tratamiento se utilizaron 2 parcelas de 8x24 m y 6 ovejas adultas raza pelibuey. La disponibilidad de biomasa en leucaena y tulipán se evaluó mediante cosecha total de 20 a 40 plantas por tratamiento. El pasto estrella se midió antes y después del pastoreo. Las 3 especies se separaron en componentes morfológicos, y se determinó proteína cruda y fibra detergente neutra en las hojas. Los hábitos de los ovinos se determinaron mediante observación directa. La disponibilidad de biomasa total, consumo por grupo y utilización del pasto estrella fueron mayores a los 70 días y en la época de lluvias. La proporción de hoja de las 3 especies fue mayor a los 50 días; por efecto de la época, fue más abundante en lluvias, nortes y seca para estrella, leucaena y tulipán, respectivamente. La disponibilidad de PC no varió (p>0,05 por efecto de frecuencia ni época de pastoreo. El mayor tiempo de consumo estuvo destinado a pasto estrella, tulipán y leucaena. Los diseños favorecieron los sistemas silvopastoriles multiasociados pues potenciaron los efectos positivos de sus relaciones. Las frecuencias de pastoreo se ajustaron a la época del año, bajo un modelo de manejo mixto de pastoreo y podas, para mejor utilización de la biomasa.

  1. Performance of biomass availability and chemical composition of 23 Leucaena spp. accessions; Comportamiento de la disponibilidad de biomasa y la composición química en 23 accesiones de Leucaena spp.

    Energy Technology Data Exchange (ETDEWEB)

    Wencomo, Hilda B., E-mail: hilda.wencomo@indio.atenas.inf.cu [Estación Experimental de Pastos y Forrajes ' Indio Hatuey' , Central España Republicana, CP 44280, Matanzas (Cuba); Ortiz, R. [Instituto Nacional de Ciencias Agrícolas, Mayabeque (Cuba)

    2012-07-01

    A study was conducted with 23 Leucaena spp. accessions in an area which had been planted six years before. The objective was to determine, in the established plants, total biomass, edible biomass, ligneous biomass, stem diameter and number of branches in each accession, and its bromatological composition under simulated grazing conditions. The research was conducted at the EEPF 'Indio Hatuey' during two years, on a hydrated ferruginous nodular humic lixiviated Ferralitic Red soil. Simple 3 m x 6 m plots were used. In the edible biomass and its components (leaves and fresh stems), there were significant differences (P<0,05) between the seasons of the two years. It could be observed that production was higher in the rainy season than in the dry season, although there were no differences among the mean biomass production of the accessions; in the case of total biomass no significant differences were found between seasons. To continue the studies is recommended, to determine, in the long term, the effect of the evaluated indicators on the availability and persistence of the tree. (author)

  2. Farinha de folha de leucena (Leucaena leucocephala Lam. de wit como fonte de proteína para juvenis de tambaqui (Colossoma macropomum CUVIER, 1818 Leucaena leaf flour (Leucaena leucocephala Lam. of wit as a protein source for juveniles of tambaqui (Colossoma macropomum Cuvier, 1818

    Directory of Open Access Journals (Sweden)

    Geraldo Pereira Junior

    2013-06-01

    Full Text Available O tambaqui (Colossoma macropomum é um peixe onívoro, natural da bacia amazônica, que possui elevado valor comercial. Características de rusticidade e desempenho produtivo destacam esta espécie para criação em cativeiro. Contudo, em criações comerciais de peixes, os custos com alimentação podem corresponder de 60 a 80% dos custos totais de produção, sendo a proteína o nutriente mais caro da dieta. O objetivo deste trabalho foi avaliar o desempenho produtivo de juvenis de tambaqui alimentados com rações contendo farinha de folha de leucena como fonte protéica. 240 juvenis foram distribuídos em 12 aquários experimentais (350 L, em um delineamento experimental inteiramente casualizado com quatro tratamentos (0%, 8%, 16%, 24% de inclusão de farinha de folha de leucena na ração e três repetições. Foram determinados o ganho de peso, conversão alimentar aparente, taxa de crescimento específico, taxa de eficiência protéica e custo de produção do quilograma de peso vivo ganho. Para as variáveis estudadas, não houve diferença significativa (p>0,05 entre os tratamentos, indicando que é possível incluir até 24% de farinha de folha de leucena em rações para juvenis de tambaqui, sem comprometimento das variáveis estudadas, embora a substituição não tenha representado redução no custo de produção do quilograma de peixe.The tambaqui (Colossoma macropomum is an omnivorous fish native to the Amazon basin, which has high commercial value. Characteristics of rusticity and desirable growth in farming highlight this species for breeding in captivity. However, in commercial fish breeding, feed costs may represent 60-80% of total costs of production, being the protein the most expensive nutrient in the diet. The objective of this study was to evaluate the growth of juvenile tambaqui fed with diets containing leucaena leaf flour as protein source. A total of 240 juveniles were distributed in 12 experimental aquaria (350 L in

  3. Effect of feed restriction on intake of Moringa oleifera and Leucaena leucocephala and growth performance of rabbits.

    Science.gov (United States)

    Santos-Ricalde, R; Gutiérrez-Ruiz, E; Novelo-Ucan, W; Martinez-Romero, P; Segura-Correa, J

    2017-12-01

    Two experiments were conducted to evaluate the effect of feed restriction on intake of Moringa oleifera (MO) or Leucaena leucocephala (LL) and growth of rabbits. In experiment one, 45 rabbits (male and female) weighing 1.18 ± 0.15 kg were used. They were randomly distributed to three feed restriction treatments (20, 30, and 40%) with 15 rabbits each (9 females and 6 males) and they were offered M. oleifera (MO) ad libitum. In experiment two, 45 growing male rabbits weighing 0.63 ± 0.113 kg were used. They were randomly assigned to 0, 20, and 30% feed restriction diets, and they have free access to L. leucocephala (LL). Intake of MO increased (P  0.05), and economic efficiency was similar among treatments. Consumption of LL increased (P  0.05). Economic efficiency improved twice in feed-restricted rabbits (2.0 and 2.3 for 20 and 30%, respectively) in contrast to that of the control 0% group (1.1). The results suggest that rabbits restricted up to 30% and supplemented with either MO or LL did not affect growth performance and reduced feed cost.

  4. Evaluation of the complexes of galactomannan of Leucaena leucocephala and Co2+, Mn2+ , Ni2+ and Zn2+

    Directory of Open Access Journals (Sweden)

    Mercê Ana Lucia R.

    2000-01-01

    Full Text Available The binding constants for the complexed species formed in aqueous solution between galactomannan of Leucaena leucocephala and the metal ions Co2+, Mn2+, Ni2+ and Zn2+ were determined by potentiometric titrations. The calculated values showed Ni2+ as the best Lewis acid towards the Lewis base -OH groups of the sugar monomers, with Zn2+ being the poorest. For all systems, a higher percentage of the complexed species was present near pH=7.0, although complexed species existed over a wide range of acidic and basic pH values. The isolated solid complexes were studied by TG-DSC thermal analysis and by EPR spectroscopy. The thermal profiles obtained showed higher thermal resistance to final degradation than the biopolymer alone for the complexed species ML having the smallest log K values. The EPR spectra confirmed the complexation of the metal ions via the Lewis base deprotonated hydroxyl groups (-O and showed that the distances between metal ions in the complexed biopolymer structure depend on the nature of the metal ion. The ability of galactomannans to complex a variety of metal ions in their web like structure and the resistance to high temperatures and a wide range of pH values of these complexes open new perspectives in possible industrial uses whenever these properties are required, such as in bioremediation of waste waters and in the application of slow-release fertilizers.

  5. Biochemical characterization of recombinant cinnamoyl CoA reductase 1 (Ll-CCRH1) from Leucaena leucocephala.

    Science.gov (United States)

    Sonawane, Prashant; Vishwakarma, Rishi Kishore; Khan, Bashir M

    2013-07-01

    Recombinant cinnamoyl CoA reductase 1 (Ll-CCRH1) protein from Leucaena leucocephala was overexpressed in Escherichia coli BL21 (DE3) strain and purified to apparent homogeneity. Optimum pH for forward and reverse reaction was found to be 6.5 and 7.8 respectively. The enzyme was most stable around pH 6.5 at 25°C for 90 min. The enzyme showed Kcat/Km for feruloyl, caffeoyl, sinapoyl, coumaroyl CoA, coniferaldehyde and sinapaldehyde as 4.6, 2.4, 2.3, 1.7, 1.9 and 1.2 (×10(6) M(-1) s(-1)), respectively, indicating affinity of enzyme for feruloyl CoA over other substrates and preference of reduction reaction over oxidation. Activation energy, Ea for various substrates was found to be in the range of 20-50 kJ/mol. Involvement of probable carboxylate ion, histidine, lysine or tyrosine at the active site of enzyme was predicted by pH activity profile. SAXS studies of protein showed radius 3.04 nm and volume 49.25 nm(3) with oblate ellipsoid shape. Finally, metal ion inhibition studies revealed that Ll-CCRH1 is a metal independent enzyme. Copyright © 2013 Elsevier B.V. All rights reserved.

  6. Evaluation of herbicides for use in transplanting leucaena leucocephala and prosopis alba on semi-arid lands without irrigation

    Energy Technology Data Exchange (ETDEWEB)

    Felker, P.; Smith, D.; Smith, M.; Bingham, R.L.; Reyes, I.

    1984-01-01

    Five herbicides were applied to plots at 2 rates in April 1982, and 3-month old seedlings planted 2 days later. Basal diameter was measured after 110 days and converted to dry weight using published equations. Percent weed cover was recorded 45, 75, and 105 days after planting. All herbicides increased survival over untreated controls. The greatest biomass production of both species was obtained with oryzalin treatment at 2.8 kg/ha active ingredient, which increased production 4-5X compared with control plots. Oryzalin was second to napropamide (2.24 kg/ha active ingredient) in grass control and equal to oxyfluorfen (1.12 kg/ha active ingredient) in forb control, oxyfluorfen at this rate also gave the second best biomass production. Oryzalin increased survival from 71 to 87% for Leucaena and from 81-94% for Prosopis, and is considered to be the best herbicide tested, followed by oxyfluorfen and metolachlor. Alachlor was considered to be too short-lived and napropamide too expensive.

  7. Acumulación de hojarasca en un pastizal de Panicum maximum y en un sistema silvopastoril de Panicum maximum y Leucaena leucocephala Litter accumulation in a Panicum maximum grassland and in a silvopastoral system of Panicum maximum and Leucaena leucocephala

    Directory of Open Access Journals (Sweden)

    Saray Sánchez

    2007-09-01

    Full Text Available Se realizó un estudio en la Estación Experimental de Pastos y Forrajes "Indio Hatuey", Matanzas, Cuba, con el objetivo de determinar la acumulación de la hojarasca en un pastizal de Panicum maximum Jacq cv. Likoni y en un sistema silvopastoril de Panicum maximum y Leucaena leucocephala (Lam de Wit cv. Cunningham. En los pastizales de P. maximum de ambos sistemas se determinó la acumulación de la hojarasca según la técnica propuesta por Bruce y Ebershon (1982, mientras que la hojarasca de L. leucocephala acumulada en el sistema silvopastoril se determinó según Santa Regina et al. (1997. De forma general, los resultados demostraron que en ambos pastizales la guinea acumuló una menor cantidad de hojarasca durante el período junio-diciembre, etapa en la que se produce su mayor desarrollo vegetativo. En la leucaena la mayor producción de hojarasca ocurrió en el período de diciembre a enero, asociada con la caída natural de sus hojas que se produce por efecto de las temperaturas más bajas y la escasa humedad en el suelo. En el sistema silvopastoril la hojarasca de leucaena representó el mayor porcentaje de peso dentro de la producción total, con un contenido más alto de nitrógeno y de calcio que el de la hojarasca del estrato herbáceo. En la guinea la lluvia fue el factor climático que mayor correlación negativa presentó con la producción de hojarasca en ambos sistemas, y en la leucaena la mayor correlación negativa se encontró con la temperatura mínima.A study was carried out at the Experimental Station of Pastures and Forages "Indio Hatuey", Matanzas, Cuba, with the objective of determining the litter accumulation in a pastureland of Panicum maximum Jacq cv. Likoni and in a silvopastoral system of Panicum maximum and Leucaena leucocephala (Lam de Wit cv. Cunningham. In the P. maximum pasturelands of both systems the litter accumulation was determined by means of the technique proposed by Bruce and Ebershon (1982, while

  8. Crotalaria incana l. and leucaena leucocephala lam. (leguminosae): toxicity indicator species of petroleum hydrocarbons in soil; Crotalaria incana l. y leucaena leucocephala lam. (leguminosae): especies indicadoras de toxicidad por hidrocarburos de petroleo en el suelo

    Energy Technology Data Exchange (ETDEWEB)

    Vazquez Luna, Dinora; Castellan Estrada, Mepivoseth; Rivera Cruz, Maria del C.; Ortiz Ceballos, Angel I.; Izquierdo R., Francisco [Colegio de Postgraduados, Campus Montecillo, Montecillo, Estado de Mexico (Mexico)]. E-mail: dinovaz@colpos.mx

    2010-07-01

    This study assesses the toxic effects produced by a Gleysol molic soil contaminated with crude oil on seedlings of two species of legumes. A phytotoxic impact index (IIF) was generated, which includes five parameters measured by relative rates of impact (IRIF{sub (x)}) for variables; emergency, height, root length, aboveground biomass and root biomass. Bioassays were conducted under a completely randomized design with three replications under semi-controlled conditions, to assess the sensitivity of Leucaena leucocephala and Crotalaria incana at different concentrations of TPH (total petroleum hydrocarbons). Effects were highly significant (P {<=} 0.01) with increasing concentrations of HTP in substrate. The emergence of L. leucocephala was 29 % lower with 80 000 mgkg{sup -1} HTP, while C. incana decreased 30 % with 32 000 mgkg{sup -1} TPH respect to control. Both species showed a five-day delay in the emergence of seedlings when exposed to high levels of TPH. A significant decrease in the accumulation of dry matter (DM) at concentrations above 20 000 mgkg{sup -1} TPH was observed in both species. The respective IIF declined of 50 % with 80 000 and 25 000 mgkg{sup -1} TPH, but L. leucocephala had no significant effect with 10 000 mgkg{sup -1} TPH. Finally, the EC50 in L. leucocephala, is presented with 80 000 mgkg{sup -1} TPH whereas in C. incana this parameter is noted from 25 000 mgkg{sup -1} TPH. [Spanish] En este estudio se evaluaron los efectos toxicos que produce un suelo Gleysol molico contaminado con petroleo crudo, sobre plantulas de dos especies de leguminosas. Para ello se genero un indice de impacto fitotoxico (IIF) que integra cinco parametros, medidos a traves de indices relativos de impacto (IRIF{sub (x)}) para las variables emergencia, altura, longitud radicular, biomasa aerea y biomasa radicular. Los bioensayos se realizaron bajo un diseno completamente al azar, con tres repeticiones, en condiciones semicontroladas, para evaluar la sensibilidad de

  9. Efecto del almacenamiento al ambiente en semillas de Leucaena leucocephala cv. Cunningham sometidas a hidratación parcial Effect of storage under ambient conditions on seeds from Leucaena leucocephala cv. Cunningham subject to partial hydration

    Directory of Open Access Journals (Sweden)

    Yolanda González

    2012-12-01

    Full Text Available El objetivo del trabajo fue estudiar el efecto del almacenamiento al ambiente en la germinación de las semillas de Leucaena leucocephala cv. Cunningham sometidas a hidratación parcial. Los tratamientos fueron: control, sin escarificación (T0; escarificación térmica (ET con H2O a 80°C, durante dos minutos (T1; ET más hidratación parcial, en bandeja con agua corriente por 28 horas (T2; ET más hidratación parcial, en saco de yute humedecido con agua corriente por 28 horas (T3; ET más hidratación parcial, en bandeja con agua corriente y TMTD al 0,1% (plaguicida por 28 horas (T4; ET más hidratación parcial, en saco de yute humedecido con agua corriente y TMTD al 0,1% (plaguicida por 28 horas (T5. Las semillas hidratadas se deshidrataron durante 72 horas al aire y a la sombra, antes de almacenarlas. Se midió la germinación y la viabilidad a 0, 1, 2, 3, 4, 6, 18, 30 y 42 meses. Se utilizó un diseño totalmente aleatorizado y cuatro réplicas por tratamiento. Hubo diferencias significativas (PThe objective of the work was to study the effect of storage under ambient conditions on the germination of seeds from Leucaena leucocephala cv. Cunningham subject to partial hydration. The treatments were: control, no scarification (T0; thermal scarification (TS with H2O at 80ºC, for two minutes (T1; TS plus partial hydration, on tray with tap water for 28 hours (T2; TS plus partial hydration, in jute sac moist with tap water for 28 hours (T3; TS plus partial hydration in tray with tap water and TMTD at 0,1% (pesticide for 28 hours (T4; TS plus partial hydration, in jute sac moist with tap water and TMTD at 0,1% (pesticide for 28 hours (T5. The hydrated seeds were dehydrated during 72 hours exposed to air and under shade, before being stored. Germination and viability were measured after 0, 1, 2, 3, 4, 6, 18, 30 and 42 months. A completely randomized design and four replications per treatment were used. There were significant differences (P<0

  10. Expression analysis of cinnamoyl-CoA reductase (CCR) gene in developing seedlings of Leucaena leucocephala: a pulp yielding tree species.

    Science.gov (United States)

    Srivastava, Sameer; Gupta, Ranadheer K; Arha, Manish; Vishwakarma, Rishi K; Rawal, Shuban K; Kavi Kishor, P B; Khan, Bashir M

    2011-02-01

    Removal of lignin is a major hurdle for obtaining good quality pulp. Leucaena leucocephala (subabul) is extensively used in paper industry in India; therefore, as a first step to generate transgenic plants with low lignin content, cDNA and genomic clones of CCR gene were isolated and characterized. The cDNA encoding CCR (EC 1.2.1.44) was designated as Ll-CCR; the sequence analysis revealed an Open Reading Frame (ORF) of 1005 bp. Phylogenetic analysis showed that Ll-CCR sequence is highly homologous to CCRs from other dicot plants. The 2992 bp genomic clone of Leucaena CCR consists of 5 exons and 4 introns. The haploid genome of L. leucocephala contains two copies as revealed by DNA blot hybridization. Ll-CCR gene was over-expressed in Escherichia coli, which showed a molecular mass of approximately 38 kDa. Protein blot analysis revealed that Ll-CCR protein is expressed at higher levels in root and in stem, but undetectable in leaf tissues. Expression of CCR gene in Leucaena increased up to 15 d in case of roots and stem as revealed by QRT-PCR studies in 0-15 d old seedlings. ELISA based studies of extractable CCR protein corroborated with QRT-PCR data. CCR protein was immuno-cytolocalized around xylem tissue. Lignin estimation and expression studies of 5, 10 and 15 d old stem and root suggest that CCR expression correlates with quantity of lignin produced, which makes it a good target for antisense down regulation for producing designer species for paper industry. Copyright © 2010 Elsevier Masson SAS. All rights reserved.

  11. Evaluación de las propiedades de materiales compuestos fabricados a partir de fibras semiquímicas de Leucaena collinsii y polipropileno

    OpenAIRE

    Granda Garcia, Luis Angel

    2016-01-01

    La presente tesis estudia el efecto que produce reforzar una fibra semiquímica de Leucaena collinsii en las propiedades mecánicas, térmicas y de sorción de agua de materiales compuestos de polipropileno. Se obtienen probetas de materiales compuestos con distinto contenido de refuerzo y se estabilizan en una cámara climática durante 48 horas antes de ser ensayadas a tracción, flexión e impacto, y se analiza la morfologia de la fractura mediante SEM. Posteriormente se estudian sus propiedad...

  12. Caracterización de diez cultivares forrajeros de Leucaena leucocephala basada en la composición química y la degradabilidad ruminal

    OpenAIRE

    García M., Danny; Wencomo G., Hilda; González C., Miriam; Medina, María; Cova O., Luis

    2008-01-01

    Objetivo. Estudiar las variaciones en la composición química y la degradabilidad ruminal de diez cultivares de Leucaena leucocephala Lam. de Wit. mediante el análisis de componentes principales (ACP). Materiales y métodos. Se tomaron muestras durante tres años para evaluar la composición química, los niveles de metabolitos secundarios y la degradabilidad ruminal en ovinos. Los datos fueron analizados con el paquete estadístico SPSS y mediante el diagrama tridimensional se obtuvieron las agrup...

  13. CARACTERIZACIÓN DE DIEZ CULTIVARES FORRAJEROS DE Leucaena leucocephala BASADA EN LA COMPOSICIÓN QUÍMICA Y LA DEGRADABILIDAD RUMINAL

    OpenAIRE

    García M, Danny; Hilda, Wencomo G; Gonzáles C, Miriam; Medina R, María; Cova O, Luis

    2008-01-01

    Objetivo. Estudiar las variaciones en la composición química y la degradabilidad ruminal de diez cultivares de Leucaena leucocephala Lam. de Wit. mediante el análisis de componentes principales (ACP). Materiales y métodos. Se tomaron muestras durante tres años para evaluar la composición química, los niveles de metabolitos secundarios y la degradabilidad ruminal en ovinos. Los datos fueron analizados con el paquete estadístico SPSS y mediante el diagrama tridimensional se obtuvieron las agrup...

  14. Ácidos grasos, fermentación ruminal y producción de metano, de forrajes de silvopasturas intensivas con Leucaena.

    Directory of Open Access Journals (Sweden)

    Esperanza Prieto-Manrique

    2016-06-01

    Full Text Available El objetivo del presente trabajo fue determinar el efecto de los forrajes sobre los ácidos grasos de cadena larga, fermentación ruminal y producción de metano de un sistema silvopastoril intensivo de Leucaena leucocephala. La investigación se desarrolló en el laboratorio NUTRILAB–GRICA, perteneciente a la Facultad de Ciencias Agrarias, de la Universidad de Antioquia, Medellín–Colombia, en julio del 2013. Se utilizó la técnica de producción de gas in vitro, utilizando como sustrato de fermentación las gramíneas (C. plectostachyus y/o M. maximus cv. Tanzania y leucaena (L. leucocephala, solas o en sus combinaciones, con una relación forraje:concentrado 70:30 y gramínea: leucaena 56:14, para un total de siete tratamientos. No se encontró efecto de los forrajes (p>0,05, sobre el contenido de ácido linoleico conjugado (ALC, C18:2 c9t11 o ruménico en la digesta. La inclusión de 14% de leucaena aumentó el contenido de ácido linoleico (C18:2 c9, 12 y linolénico (C18:3 c9, 12, 15 en el alimento y de transvaccénico (ATV, C18:1 t11, esteárico (C18:0, linoleico y linolénico en la digesta (p<0,05, y no afectó la cinética de fermentación, digestibilidad de la materia seca (MS, pH, total y proporción de ácidos grasos volátiles, ni redujo la producción de metano. Las gramíneas, C. plectostachyus y M. maximus, se comportaron similar en las variables evaluadas (p>0,05. Los sistemas silvopastoriles pueden ser una opción para aumentar los ácidos grasos benéficos en la leche.

  15. Anatomy and ultrastructure alterations of Leucaena leucocephala (Lam.) inoculated with mycorrhizal fungi in response to arsenic-contaminated soil

    Energy Technology Data Exchange (ETDEWEB)

    Schneider, Jerusa, E-mail: jerusaschneider@hotmail.com [Departamento de Ciência do Solo, Universidade Federal de Lavras (UFLA), PO Box 3037, Lavras, Minas Gerais, 37200-000 (Brazil); Labory, Claudia Regina Gontijo [Departamento de Fitopatologia, Universidade Federal de Lavras, PO Box 3037, Lavras, Minas Gerais, 37200-000 (Brazil); Rangel, Wesley Melo [Departamento de Ciência do Solo, Universidade Federal de Lavras (UFLA), PO Box 3037, Lavras, Minas Gerais, 37200-000 (Brazil); Alves, Eduardo [Departamento de Fitopatologia, Universidade Federal de Lavras, PO Box 3037, Lavras, Minas Gerais, 37200-000 (Brazil); Guilherme, Luiz Roberto Guimarães [Departamento de Ciência do Solo, Universidade Federal de Lavras (UFLA), PO Box 3037, Lavras, Minas Gerais, 37200-000 (Brazil)

    2013-11-15

    Highlights: ► Inoculation of L. leucocephala improved plant growth in high-As soils. ► Plants inoculated with Glomus clarum were less sensitive to As. ► Ultrastructural changes in leaves of L. leucocephala. ► Modified structures in intracellular spaces in plants inoculated with G. clarum. ► Cell disruption and stacking of root cell walls at high As concentrations. -- Abstract: Many studies demonstrate the potential application of arbuscular mycorrhizal fungi (AMF) for remediation purposes, but little is known on AMF potential to enhance plant tolerance to arsenic (As) and the mechanisms involved in this process. We carried anatomical and ultrastructural studies to examine this symbiotic association and the characteristics of shoots and roots of Leucaena leucocephala in As-amended soils (35 and 75 mg As dm{sup −3}). The experiment used 3 AMF isolates from uncontaminated soils: Acaulospora morrowiae, Glomus clarum, and Gigaspora albida; a mixed inoculum derived from combining these 3 isolates (named Mix AMF); and, 3 AMF isolates from As-contaminated areas: A. morrowiae, G. clarum and Paraglomus occultum. Phytotoxicity symptoms due to arsenic contamination appeared during plant growth, especially in treatments without AMF application. Inoculation with G. clarum and the mixture of species (A. morrowiae, G. albida, and G. clarum) resulted in better growth of L. leucocephala in soils with high As concentrations, as well as significant As removal from the soil, showing a potential for using AMF in phytoextraction. Light microscopy (LS), transmission (TEM) and scanning electron microscopies (SEM) studies showed the colonization of the AMF in plant tissues and damage in all treatments, with ultrastructural changes being observed in leaves and roots of L. leucocephala, especially with the addition of 75 mg dm{sup −3} of As.

  16. Estimating Apparent Nutrient Digestibility of Diets Containing Leucaena leucocephala or Moringa oleifera Leaf Meals for Growing Rabbits by Two Methods.

    Science.gov (United States)

    Safwat, A M; Sarmiento-Franco, L; Santos-Ricalde, R H; Nieves, D; Sandoval-Castro, C A

    2015-08-01

    This study aimed to evaluate the nutrient digestibility of growing rabbits fed diets with different levels of either Leucaena leucocephala (LLM) or Moringa oleifera (MOLM) leaf meals and also to compare total collection and TiO2 marker methods for estimating digestibility. A total of 30 California growing rabbits (1.81±0.19 kg live weight on average) were randomly distributed into five experimental groups of six rabbits each and were housed in individual cages. The groups were control, 30% LLM, 40% LLM, 30% MOLM, and 40% MOLM. All groups received pelleted diets for two weeks; diets also contained 4 g/kg titanium dioxide as dietary marker. Daily feed intake was recorded during the whole experimental period and total feces were collected daily and weighed individually during four days. The results showed that there were no difference (p>0.05) in feed, dry matter (DM), organic matter (OM), crude protein (CP), digestible energy, and crude fiber (CF) intake between the control group and the other experimental groups. The apparent digestibility values of DM, OM, CP, CF, acid detergent fiber, and gross energy were the highest for control group (p = 0.001), meanwhile MOLM diets had generally higher nutrient digestibility coefficients than LLM diets. Increasing the inclusion level of leaf meal in the diet from 30% to 40% improved the digestibility of CF from 45.02% to 51.69% for LLM and from 48.11% to 55.89% for MOLM. Similar results for apparent nutrient digestibility coefficients were obtained when either total collection or indigestible marker method was used. In conclusion, the digestibility of MOLM containing diets were better than LLM diets, furthermore TiO2 as an external marker could be used as a simple, practical and reliable method to estimate nutrients digestibility in rabbit diets.

  17. Proliferation of the biocontrol agent Fusarium oxysporum f. sp. strigae and its impact on indigenous rhizosphere fungal communities in maize under different agro-ecologies.

    Science.gov (United States)

    Zimmermann, Judith; Musyoki, Mary K; Cadisch, Georg; Rasche, Frank

    2016-06-01

    Our objectives were to (1) monitor the proliferation of the biocontrol agent (BCA) Fusarium oxysporum f. sp. strigae strain "Foxy-2", an effective soil-borne BCA against the parasitic weed Striga hermonthica , in the rhizosphere of maize under different agro-ecologies, and (2) investigate its impact on indigenous rhizosphere fungal community abundance and composition. Field experiments were conducted in Busia and Homa Bay districts in western Kenya during two cropping seasons to account for effects of soil type, climate, growth stage and seasonality. Maize seeds were coated with or without "Foxy-2" and soils were artificially infested with S. hermonthica seeds. One treatment with nitrogen rich organic residues ( Tithonia diversifolia ) was established to compensate hypothesized resource competition between "Foxy-2" and the indigenous fungal community. Rhizosphere soil samples collected at three growth stages (i.e., EC30, EC60, EC90) of maize were subjected to abundance measurement of "Foxy-2" and total indigenous fungi using quantitative polymerase chain reaction (qPCR) analysis. Terminal restriction fragment length polymorphism (TRFLP) analysis was used to assess potential alterations in the fungal community composition in response to "Foxy-2" presence. "Foxy-2" proliferated stronger in the soils with a sandy clay texture (Busia) than in those with a loamy sand texture (Homa Bay) and revealed slightly higher abundance in the second season. "Foxy-2" had, however, only a transient suppressive effect on total indigenous fungal abundance which ceased in the second season and was further markedly compensated after addition of T. diversifolia residues. Likewise, community structure of the indigenous fungal community was mainly altered by maize growth stages, but not by "Foxy-2". In conclusion, no adverse effects of "Foxy-2" inoculation on indigenous fungal rhizosphere communities were observed corroborating the safety of this BCA under the given agro-ecologies.

  18. SP. Pescado

    Directory of Open Access Journals (Sweden)

    Renato Gendre

    2003-12-01

    Full Text Available Nell'occhiello di un articolo dal titolo Il Peru dei de[Jini rosa e de/la grande pioggia si legge: "da una partenza  in aereo al «pescado»  che ti  sfamera."1 Questa parola spagnola, giustamente chiusa tra caporali, a noi pare molto interes­ sante, perche, nonostante l'apparenza, non ha nulla da spartire sotto i1 profilo se­ mantico con l'it. pescato. lnfatti, tutti i piu importanti dizionari della lingua italiana, di ieri e di oggi, etimologici e non 2, registrano  accanto a pescata,  ii lemma pescato, 3 ma lo spiegano come "quantita di pesce catturato nel corso di una battuta o di una stagione di pesca",4 mentre lo sp. pescado  indica i1 "pesce (solo nel senso di: pesGe pescato da mangiare [...]".s

  19. INFLUÊNCIA DE TRATAMENTOS PRÉ-GERMINATIVOS, TEMPERATURA E LUMINOSIDADE NA GERMINAÇÃO DE SEMENTES DE LEUCENA (Leucaena leucocephala (Lam. De Wit., cv. CUNNINGHAM

    Directory of Open Access Journals (Sweden)

    Alexandre Bosco de Oliveira

    2009-01-01

    Full Text Available The research was lead in two experiments with the objective of to identify methods to superation of dormancy in seeds of Leucaena leucocephala, Cunningham variety, and to verify the effect of light and temperatures in germination of this species. In first the seeds were submitted the nine methods to superation of dormancy: immersion in water under ambient temperature during 24, 48 and 72 hours, immersion in hot water under temperatures of 60, 80 and 100oC, immersion in sulfuric acid per 5 and 10 minutes, beyond of the witness. In second experiment, after treatment with sulfuric acid for 10 minutes, the seeds were sowed in tabledoch paper type Germitest and put to germinate under ten combinations: continue light and constant temperature of 20, 25, 30 and 35oC; dark continue and constant temperature of 20, 25, 30 and 35oC; alternated temperature of 20-30 and 25-35, both with fotoperiod of eight hours. Conclusion the specie Leucaena leucocephala presents seeds dormancy, detach-itself the sulfuric acid as efficient method to the superation of dormancy; the seeds of leucena are insensitive to the light and your germination was not influenced by the temperatures useds.

  20. Caracterización de la comunidad vegetal en una asociación de gramíneas mejoradas y Leucaena leucocephala cv.: Cunningham Characterization of the plant community in an association of improved grasses and Leucaena leucocephala cv.: Cunningham

    Directory of Open Access Journals (Sweden)

    Tania Sánchez

    2007-11-01

    Full Text Available En una vaquería de la Empresa Genética de Matanzas se realizó un estudio durante cinco años, con el objetivo de caracterizar el comportamiento de la comunidad vegetal en una asociación de gramíneas mejoradas y Leucaena leucocephala cv. Cunningham en condiciones comerciales. Se determinó la composición botánica del pastizal, la densidad de arbóreas, la disponibilidad de materia seca y los contenidos de MS, PB, Ca y P de las especies presentes en el sistema. La composición botánica del pastizal se caracterizó por un porcentaje de pastos mejorados superior al 50%, con predominio de Cynodon nlemfuensis cv. Jamaicano y Panicum maximum cv. Likoni; mientras que la leucaena mantuvo la densidad de plantas, como una adaptación a las condiciones del sistema. La disponibilidad de materia seca total fue superior a las 3 t/ha/rotación durante la etapa experimental. Se concluye que en la asociación de gramíneas mejoradas y L. leucocephala cv. Cunningham se presentó una alta disponibilidad de materia seca y persistencia del pastizal durante los cinco años del estudio, con valores de PB en las gramíneas mejoradas de 9,6-9,8% y en la leucaena de 25%, sin la aplicación de riego ni fertilizantes químicos, lo cual permitió atenuar las diferencias entre los períodos lluvioso y poco lluvioso.In a dairy of the Genetic Enterprise of Matanzas, a work was carried out for five years, with the objective of characterizing the performance of the plant community in an association of improved grasses and Leucaena leucocephala cv Cunningham under commercial conditions. The botanical composition of the pastureland, tree density, dry matter availability and DM, CP, Ca and P contents of the species present in the system were determined. The botanical composition of the pastureland was characterized by a percentage of improved pastures higher than 50%, with predominance of Cynodon nlemfuensis cv. Jamaicano and Panicum maximum cv. Likoni; while leucaena

  1. Draft genome sequences of six neonatal meningitis-causing escherichia coli isolates (SP-4, SP-5, SP-13, SP-16, SP-46, and SP-65)

    Science.gov (United States)

    Neonatal meningitis Escherichia coli isolates (SP-4, SP-5, SP-13, SP-16, SP-46, and SP-65) were recovered from infants in the Netherlands from 1989 to 1997. Here, we report the draft genome sequences for these six E. coli isolates, which are currently being used to validate food safety processing te...

  2. Efecto de la suplementación con residuos de destilería del maíz en el comportamiento de novillas en una asociación de gramínea y leucaena Effect of supplementation with corn distillery residues on the performance of heifers in an association of grass and leucaena

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    Tania Sánchez

    2010-09-01

    Full Text Available El objetivo del presente trabajo fue evaluar el efecto de la suplementación con residuos de destilería del maíz en el comportamiento de novillas, en una asociación de gramínea y leucaena. El estudio se realizó en un área de la Estación Experimental "Indio Hatuey", en el municipio de Perico, provincia de Matanzas, Cuba. La especie de pasto predominante fue Panicum maximum cv. Likoni y como leguminosa Leucaena leucocephala. Se estudiaron 18 novillas, distribuidas en un diseño completamente aleatorizado en tres grupos de seis animales; el grupo control no recibió suplementación (A y los otros dos grupos se suplementaron con residuos de destilería del maíz en un 10% (B y 20% (C de los requerimientos de proteína con relación al peso vivo. Se determinó la composición química, la disponibilidad de materia seca, la ganancia de peso vivo, la infestaci��n parasitaria y el hematocrito. Al analizar las ganancias diarias por tratamiento se encontraron diferencias significativas (PThe objective of this work was to evaluate the effect of supplementation with corn distillery residues on the performance of heifers in an association of grass and leucaena. The study was conducted in an area of the Experimental Station "Indio Hatuey" , Perico municipality, Matanzas province, Cuba. The prevailing pasture species was Panicum maximum cv. Likoni and as legume, Leucaena leucocephala. Eighteen heifers, distributed in a completely randomized design in three groups of six animals, were used; the control group did not receive supplementation (A, and the other two groups were supplemented with corn distillery residues in 10% (B and 20% (C of the protein requirements with regards to live weight. The chemical composition, dry matter availability, live weight gain, parasite infestation and hematocrit were determined. When analyzing the daily gain per treatments, significant differences (P<0,05 were found and the highest values were obtained in treatment C

  3. First report of Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Lecanodiaspididae and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae in Brazil

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    A. L. Marsaro Júnior

    Full Text Available Abstract Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Coccoidea: Lecanodiaspididae and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae are reported for the first time in Brazil. Specimens of this scale insect were collected on branches and stems of Acacia mangium Willd., Leucaena leucocephala (Lam. de Wit (Fabaceae, Morus nigra L. (Moraceae, Citrus reticulata Blanco (Rutaceae, Tectona grandis L. f. (Verbenaceae, Anacardium occidentale (Anacardiaceae, Annona squamosa L. and Xylopia aromatica (Lam. Mart. (Annonaceae, in three municipalities of the Roraima state. All plants here mentioned are recorded for the first time as a host for L. dendrobii. Morphological characters of L. dendrobii and symptoms presented by the host plants infested by this pest are included in this work.

  4. [Nutritional evaluation of the seeds of "ipil-ipil" (Leucaena leucocephala), "casco de vaca" (Bauhinia monandra) and scented carob (Albizia lebbeck)].

    Science.gov (United States)

    Abreu Peñate, M; Bencomo Hernández, A; Sampere Díaz, E; Farrás Fernández, I; Hernández Triana, M; Porrata Maury, C; Ponce de León Boloy, I

    1988-12-01

    Chemical composition, antinutritional factors and biological protein quality of raw and autoclaved (120 degrees C for 10 min) ipil-ipil (Leucaena leucocephala), casco de vaca (Bauhinia monandra) ard algarrobo de olor (Albizia lebbeck) seeds were determined. The trypsin inhibitor activity was 29, 120, and 150 TIU/mg of sample respectively. Autoclaving eliminated 59, 33 and 100% of the activity. Bauhinia monandra seed depicted a high hemagglutinating activity which was eliminated by autoclaving. Net protein utilization of raw and autoclaved seeds was 31 and 30, 21 and 55 and 29 and 49%, respectively. All seeds were free from cyanide and alkaloids. It is concluded that the relative low protein quality of the seeds would limit their use for human consumption.

  5. Nutritional Characteristics of Important Dwarf Shrubs and Value of Leucaena leueocephala seeds as Protein Supplement for Goats in Marsabit District, Kenya

    International Nuclear Information System (INIS)

    Kuria, S.G; Karue, C.N

    1999-01-01

    A study on the nutritional characteristics of Indigofera spinosa and Duosperma eremophilum dwarf shrubs was carried out in Southwest Marsabit district of Kenya to establish the digestibility intake by goats and their chemical composition for management purposes. The value of Leucaena leucocephala seeds as protein supplement for goats was also tested where three levels, 0 (control), 50 and 100 g/goat/day were used. Intake and digestibility of the shrubs were assessed by difference and conventional methods respectively. Proximate analysis and Van Soest fibre analysis were done for chemical composition. Goats on l. spinosa had 330+ or -34. 5 g/day Dry Matter Intake (DMI) while those on D. eremophilum had 175+or-89.0 g/day. Dry Matter of l. spinosa was 40.3+or-2.9 % digestible while that of D. eremophilum was 45.0+or-6.6 % digestible. Indigofera spinosa contained 8.8 % Crude Protein (CP), 55.4 % Neutral Detergent Fibre (NDF) and 4.1 KCal/gDM energy compared to 7.6 % CP, 29.3 % Neutral Detergent Fibre (NDP) and 3.1 KCal/gDM energy for D. eremophililum. In reference to the controls, DMI increased by 124g and 55g for D. eremophilum and I. spinosa respectively for every 50g increase in L. lecocephala seeds for l. spinosa. From the results it was concluded that in terms of intake, digestibility and CP, both shrubs are medium quality. Leucaena leucocephala seeds are rich in CP (26.0%) and increased both DMI and DMD for the shrubs. It is thus suitable for use as protein supplement in small ruminant feeding especially during the dry season when it will increase intake and digestibility of the shrubs

  6. Descomposición de la hojarasca en un sistema silvopastoril de Panicum maximum y Leucaena leucocephala (Lam de Wit cv. Cunningham: I. Influencia de su composición química Litter decomposition in a silvopastoral system of Panicum maximum and Leucaena leucocephala (Lam de Wit cv. Cunningham: I. Influence of their chemical composition

    Directory of Open Access Journals (Sweden)

    Saray Sánchez

    2009-09-01

    Full Text Available Con el objetivo de determinar la descomposición de la hojarasca y su relación con la composición química de Panicum maximum y Leucaena leucocephala en un sistema silvopastoril, se realizó este experimento en la EEPF "Indio Hatuey". La descomposición de la hojarasca se determinó como la pérdida de biomasa a través del tiempo, con relación al peso inicial. Para el estudio de la dinámica de la descomposición se utilizó el método de bolsas de hojarasca (litter bags. En cada especie se presentó un patrón diferente de descomposición de la hojarasca; la tasa promedio de descomposición de la hojarasca en leucaena fue mayor que en la guinea. En ambas especies se encontró una rápida pérdida de peso durante los primeros 30 días y después el proceso fue más lento. Este comportamiento puede estar relacionado con la composición química de los pastos, pues el porcentaje de biomasa perdida de la hojarasca de L. leucocephala presentó una mayor correlación con las concentraciones del contenido celular, la relación lignina/nitrógeno, la celulosa y el Nt. Se observó una dependencia significativa y negativa de la hojarasca de P. maximum con las concentraciones de la FND y la hemicelulosa; mientras que se relacionó de forma positiva con las de N-FND y la relación lignina/nitrógenoWith the objective of determining the litter decomposition and its relationship to the chemical composition of Panicum maximum and Leucaena leucocephala in a silvopastoral system, this trial was conducted at the EEPF «Indio Hatuey». Litter decomposition was determined as biomass loss through time, with regards to initial weight. For studying the decomposition dynamics, the method of litter bags was used. In each species a different litter decomposition pattern appeared; the average litter decomposition rate was higher in leucaena than in Guinea grass. Rapid weight loss was found in both species during the first 30 days and afterwards the process was slower

  7. Descomposición de la hojarasca en un sistema silvopastoril de Panicum maximum y Leucaena leucocephala (Lam de Wit cv. Cunningham: II. Influencia de los factores climáticos Litter decomposition in a silvopastoral system of Panicum maximum and Leucaena leucocephala (Lam de Wit cv. Cunningham: II. Influence of climatic factors

    Directory of Open Access Journals (Sweden)

    Saray Sánchez

    2009-12-01

    Full Text Available Con el objetivo de determinar la descomposición de la hojarasca en un sistema silvopastoril de Panicum maximum y Leucaena leucocephala y su relación con algunos factores del clima, se realizó este experimento en la EEPF «Indio Hatuey». La descomposición de la hojarasca se determinó como la pérdida de biomasa a través del tiempo, con relación al peso inicial. Para el estudio de la dinámica de la descomposición se utilizó el método de bolsas de hojarasca (litter bags; se registró diariamente el comportamiento de la temperatura media, la humedad relativa, la precipitación y los días con lluvias, en la estación metereológica situada a 1 km del área experimental. Se utilizó el análisis de correlación y regresión para conocer la interrelación entre las variables y los modelos de mejor ajuste. Se consideró, como variables independientes, los factores climáticos estudiados, y como variable dependiente el porcentaje de biomasa perdida. De forma general, los resultados demostraron que el comportamiento de la descomposición de la hojarasca, tanto en la guinea como en la leucaena, estuvo relacionado con los factores climáticos que prevalecieron durante el período experimental y, por tanto, es posible explicar este proceso en ambos pastizales a partir de la acción conjunta de la temperatura, la humedad relativa y la precipitación.With the objective of determining the litter decomposition in a silvopastoral system of Panicum maximum and Leucaena leucocephala and its relationship to some climate factors, this trial was conducted at the EEPF «Indio Hatuey». Litter decomposition was determined as the loss of biomass through time, with regards to initial weight. For studying the decomposition dynamics the method of litter bags was used; the performance of mean temperature, relative humidity, rainfall and days with rain, was daily recorded at the meteorological station located 1 km away from the experimental area. . The

  8. Comportamiento productivo de vacas lecheras Mambí de Cuba en una asociación de gramíneas y Leucaena leucocephala cv. Cunningham Productive performance of Cuban Mambí dairy cows in an association of grasses and Leucaena leucocephala cv. Cunningham

    Directory of Open Access Journals (Sweden)

    Tania Sánchez

    2008-12-01

    Full Text Available Se evaluó el comportamiento de los indicadores productivos de vacas Mambí (¾ Holstein x ¼ Cebú de Cuba en una asociación de gramíneas mejoradas y Leucaena leucocephala cv. Cunningham, en una vaquería de la Empresa Genética de Matanzas. El pesaje de leche se realizó durante dos años al 100% de los animales en ordeño, con una frecuencia mensual. Se determinó la producción de leche por vaca en ordeño y la producción por vaca total, así como la influencia del bimestre de producción, la época del año, el número de la lactancia y el año, en el comportamiento productivo. Se alcanzó coincidencia entre la curva real y potencial de producción de leche, con una eficiencia superior al 85%; los mayores valores se obtuvieron en el período lluvioso. Al analizar la producción de leche por bimestre los mejores resultados se alcanzaron en julio-agosto. Además se lograron valores de producción por lactancia de 2 030-2 159 kg y por hectárea de 2 744-3 025 kg. A su vez, cuando se analizó el efecto de la época del año en el peso de los terneros al nacer no se encontraron diferencias significativas; los valores fueron superiores a 37,5 kg para ambos períodos. Se concluye que con la asociación de gramíneas mejoradas y leucaena se obtuvieron producciones totales, por lactancia y por hectárea aceptables, lo cual conllevó un adecuado nivel de rentabilidad en la vaquería, con ganancias superiores a los 1 000 pesos por hectárea.The performance of the productive indicators of Mambí cows (3/4 Holstein x ¼ Zebu from Cuba was evaluated in an association of improved grasses and Leucaena leucocephala cv. Cunningham, at a dairy unit of the Genetic Livestock Production Enterprise of Matanzas. The milk weighing was performed for two years in 100% of the milking animals, with a monthly frequency. Milk production per milking cow and total production per cow were determined, as well as the influence of production two-month period, season

  9. Chemical elements in Leucaena leucocephala leaves of riparian zones of the municipality of Piracicaba, São Paulo, Brazil; Elementos químicos em folhas de Leucaena leucocephala de zonas ripárias do município de Piracicaba, São Paulo, Brasil

    Energy Technology Data Exchange (ETDEWEB)

    França, Elvis J. de; Fernandes, Elisabete A.N.; Lira, Marcelo G.; Ferreira, Fabiano S.; Cavalca, Isabel P.O.; Rodrigues, Vanessa S.; Camilli, Leandro, E-mail: ejfranca@yahoo.com.br, E-mail: liramarcelo@hotmail.com, E-mail: biologofabiano10@gmail.com [Centro Regional de Ciências Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil)

    2017-07-01

    The species Leucaena leucocephala can be found in several riparian ecosystems, acting as one of the main invasive and harmful species for the forest restoration of the permanent preservation areas. It has also been studied for the phytoremediation of some chemical elements, due to their potential accumulation of chemical substances. Therefore, the present study aimed to evaluate the accumulation of chemical elements in leaves of Leucaena leucocephala trees by Instrumental Neutronic Activation Analysis - INAA. Samples of leaves were collected in eight samples from riverside areas of the Piracicaba Municipality, São Paulo, Brazil, during the dry and rainy season. After collection, washing with water and drying in a forced circulation oven, the samples were comminuted and encapsulated in polyethylene capsules and subjected to a thermal neutron flux of 10{sup 13} cm{sup -2}s{sup -1} for 8 hours in the Nuclear Research Reactor IEA-R1 of IPEN / CNEN. Thermal neutron flux monitoring was performed by Ni-Cr alloy fragments with known concentrations of the chemical monitors elements. After measurements of radioactivity induced by HPGe detectors, the concentrations of the chemical elements were calculated by the k0 method using the Quantu computer program. Certified reference materials were also analyzed for quality assurance of the analytical procedure. The results indicated high concentrations of La and lanthanoids (Ce, Eu, Sm, Tb and Yb) in leaves of L. leucocephala in both periods analyzed. It was possible to observe a decrease in the concentrations of the chemical elements in the rainy season, probably associated to the washing of the geological material deposited on the leaves of the trees by the rains, except for K, Mo, Rb, Sb and Zn, whose values remained or increased in the leaves collected during the rainy season. Considering the constant leaf production, L. leucocephala can greatly affect the cycling of chemical elements due to its accumulation capacity

  10. INCLUSIÓN DE HARINAS DE FOLLAJES ARBÓREOS Y ARBUSTIVOS TROPICALES (Morus alba, Erythrina poeppigiana, Tithonia diversifolia EHibiscus rosa-sinensis EN LA ALIMENTACIÓN DE CUYES (Cavia porcellus Linnaeus

    Directory of Open Access Journals (Sweden)

    G. A. Meza

    2014-01-01

    Full Text Available La investigación se ejecutó en la Finca Experimental “La María” propiedad de la Univer-sidad Técnica Estatal de Quevedo (UTEQ localizada en el km 71/2de la vía Quevedo-Mocache; Provincia de Los Ríos cuya ubicación geográfica de 1º 6’ 23” de latitud sury 79º 29’ 12” de longitud oeste y a una altura de 73 m.s.n.m. El objetivo principal fueevaluar el comportamiento productivo de cuyes con la inclusión del 20% de harinasderivadas de follajes arbustivos y arbóreos tropicales. Se utilizaron 40 cuyes macho de 30días de edad. Se empleó un diseño completamente al azar con cinco tratamientos, cuatroréplicas y la unidad experimental estuvo conformado por dos cuyes. Para determinarlas diferencias entre medias de tratamientos se aplicó la prueba de Tukey (P≤0.05. Seevaluaron cinco dietas-tratamientos: (T0 dieta 100% balanceado, (T1 80% dieta y 20%harina deMorus alba, (T2 80% dieta y 20% harina deErythrina poeppigiana, (T3 80%dieta y 20% harina deTithonia diversifolia, (T4 80% dieta y 20% harina deHibiscusrosa-sinensis. Las variables bajo estudio fueron: consumo de alimento de balanceado enmateria seca (CABMS, g, ganancia de peso (GP, g, índice de conversión alimenticia(ICA y rendimiento en canal (RC, %. La rentabilidad de los tratamientos se determinóa través de la relación beneficio-costo (R b/c. Los mayores (P<0.01 CABMS, GP-ICAy RC-Rentabilidad, la registraron los tratamientos: T0 (48.34 g MS animal-1d-1, T1(8.80 g animal-1d-1y 5.04 y el T3 (77.67% y 26.20%, respectivamente.

  11. Effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on in vitro methane production and rumen fermentation.

    Science.gov (United States)

    Saminathan, Mookiah; Sieo, Chin Chin; Abdullah, Norhani; Wong, Clemente Michael Vui Ling; Ho, Yin Wan

    2015-10-01

    Molecular weights (MWs) and their chemical structures are the primary factors determining the influence of condensed tannins (CTs) on animal nutrition and methane (CH4 ) production in ruminants. In this study the MWs of five CT fractions from Leucaena leucocephala hybrid-Rendang (LLR) were determined and the CT fractions were investigated for their effects on CH4 production and rumen fermentation. The number-average molecular weight (Mn ) of fraction F1 (1265.8 Da), which was eluted first, was the highest, followed by those of fractions F2 (1028.6 Da), F3 (652.2 Da), F4 (562.2 Da) and F5 (469.6 Da). The total gas (mL g(-1) dry matter (DM)) and CH4 production decreased significantly (P fractions, but there were no significant (P > 0.05) differences between the CT fractions and control on DM degradation. However, the in vitro N disappearance decreased significantly (P fraction F1 (highest MW) compared with the control and other fractions (F2-F5). The inclusion of CT fraction F1 also significantly decreased (P fraction F1 but not by the control and other fractions (F2-F5). The CT fractions of different MWs from LLR could affect rumen fermentation and CH4 production, and the impact was more pronounced for the CT fraction with a higher MW. © 2014 Society of Chemical Industry.

  12. The first assessment of the stress inducible defense of Leucaena leucocephala with acaricidal potential effect against Rhipicephalus (Boophilus microplus (Acari: Ixodidae

    Directory of Open Access Journals (Sweden)

    Lêdia Feitosa Wanderley

    Full Text Available Abstract Plants respond to wounding caused by mechanical stress or herbivory by synthesizing defense proteins. There are no studies reporting the action of induced plant proteins against ticks. The aim of this study was to investigate the effect of mechanically wounded Leucaena leucocephala leaves against Rhipicephalus (Boophilus microplus. Initially, we carried out time course experiments to evaluate the impact of mechanical wounding on the protein content and the peroxidase, catalase and protease inhibitor activities in L. leucocephala. We then evaluated the acaricidal activity on R. (B. microplus from protein extract collected from L. leucocephala after mechanical wounding. L. leucocephala leaves were artificially wounded, and after 6, 12, 24 and 48h, the leaves were collected for protein extraction. Quantitative and qualitative analyses of the proteins were performed. The protein content and peroxidase and protease activities increased 12h after wounding, and the acaricidal activity of this protein extract was evaluated using engorged R. (B. microplus females. The protein extract obtained after wounding reduced egg production (8.5% compared to those without wounding. Furthermore, the extract reduced egg hatching by 47.7% and showed an overall efficacy of 56.3% at 0.1 mgP/mL of the protein. We demonstrated that L. leucocephala defensive proteins could be effective against R. (B. microplus.

  13. Abiotic stress induces change in Cinnamoyl CoA Reductase (CCR) protein abundance and lignin deposition in developing seedlings of Leucaena leucocephala.

    Science.gov (United States)

    Srivastava, Sameer; Vishwakarma, Rishi K; Arafat, Yasir Ali; Gupta, Sushim K; Khan, Bashir M

    2015-04-01

    Aboitic stress such as drought and salinity are class of major threats, which plants undergo through their lifetime. Lignin deposition is one of the responses to such abiotic stresses. The gene encoding Cinnamoyl CoA Reductase (CCR) is a key gene for lignin biosynthesis, which has been shown to be over-expressed under stress conditions. In the present study, developing seedlings of Leucaena leucocephala (Vernacular name: Subabul, White popinac) were treated with 1 % mannitol and 200 mM NaCl to mimic drought and salinity stress conditions, respectively. Enzyme linked immunosorbant assay (ELISA) based expression pattern of CCR protein was monitored coupled with Phlorogucinol/HCl activity staining of lignin in transverse sections of developing L. leucocephala seedlings under stress. Our result suggests a differential lignification pattern in developing root and stem under stress conditions. Increase in lignification was observed in mannitol treated stems and corresponding CCR protein accumulation was also higher than control and salt stress treated samples. On the contrary CCR protein was lower in NaCl treated stems and corresponding lignin deposition was also low. Developing root tissue showed a high level of CCR content and lignin deposition than stem samples under all conditions tested. Overall result suggested that lignin accumulation was not affected much in case of developing root however developing stems were significantly affected under drought and salinity stress condition.

  14. Characterization of chemical elements of fruits of Leucaena leucocephala in riverside areas of the Piracicaba River Basin, São Paulo, Brazil

    International Nuclear Information System (INIS)

    França, Elvis J.; Magalhães, Marcelo R.L. de; Ferreira, Fabiano S.; Fernandes, Elisabete A. de N.; Fonseca, Felipe Y.; Rodrigues, Vanessa S.; Cavalca, Isabel P.O.; Camilli, Leandro; Silva, Bruno F.

    2017-01-01

    The species Leucaena leucocephala is present in several environments. Its fruit is widely used in cattle feed. However, it is classified as an invasive species, causing problems in the restoration of impacted areas. The Piracicaba River Basin is heavily affected by urbanization and industrialization, which shows the proliferation of this species. Considering the importance of this tree species for Brazil, the present study quantifies chemical elements in fruits of this species by applying Instrumental Neutronic Activation Analysis, method k 0 . Samples were collected in riverside areas of the Piracicaba Municipality, São Paulo, Brazil, at different periods (dry and rainy season). After collection, the samples were shattered and transferred to polyethylene capsules and irradiated with neutrons. Certified reference materials were used to evaluate the quality of the analytical procedure, all material was irradiated under a thermal neutron flux of 10 13 cm-2s-1 for 8 hours. Ni-Cr alloy was used for the monitoring of thermal neutron flux. High Resolution Range Spectrometry with HPG detectors was employed for the measurement of the induced radioactivity, allowing the quantification of the chemical elements. Compared with available results of chemical analysis of pods, the chemical elements Ca, K, Ba, Cr and Se presented high concentrations. We also quantified chemical elements of environmental relevance such as As, Hg, Br, Ce, Cs, Eu, Hf, La, Rb, Sb, Sc, Sm, Ta, Tb, Th and Yb, indicating the need for element dynamics studies in the environments occupied by this invasive species

  15. Curvas de índice de sítio para leucena [Leucaena leucocephala (Lam. De Wit] no agreste de Pernambuco

    Directory of Open Access Journals (Sweden)

    Emanuell Florencio Passos Martins

    2007-01-01

    Full Text Available The main goal of this work was to set and compare site index curves for leucena [Leucaena leucocephala (Lam. de Wit] variety Hawaii (cv K8 in Agreste of Pernambuco. The data set came from Empresa Pernambucana de Pesquisa Agropecuária (IPA, Experimental Station of Caruaru, in which 544 trees cultivated with and without organic compound were measured 19 times during the period of 1990 – 2003. The models of Schumacher, Chapman-Richards, Silva-Bailey, Mitscherlich, Weibull and Clutter-Jones were tested and compared. To select the best equations, the Index of Fit (IF, standard error of estimate (S yx %, identity model test and the graphic of residuals distribution were used and results show that the model of Clutter-Jones provided a better IF for both treatments. The graphic of residual distribution did not show tendency among the models. The model identity test showed no differences among the models tested. As Schumacher is model has been used frequently in forestry and presented the smallest number of parameters, it deserves special focus in studies of site index.

  16. ExaSP2

    Energy Technology Data Exchange (ETDEWEB)

    2017-09-08

    ExaSP2 is a reference implementation of typical linear algebra algorithms and workloads for a quantum molecular dynamics (QMD) electronic structure code. The algorithm is based on a recursive second-order Fermi-Operator expansion method (SP2) and is tailored for density functional based tight-binding calculations of material systems.

  17. Stimulative effect of the fungal biocontrol agent Fusarium oxysporum f.sp. Striga on abundance of nitrifying prokaryotes in a maize rhizosphere

    Science.gov (United States)

    Musyoki, Mary; Enowashu, Esther; Zimmermann, Judith; Muema, Esther; Wainright, Henry; Vanlauwe, Bernard; Cadisch, Georg; Rasche, Frank

    2014-05-01

    The integration of resistant crop varieties and Fusarium oxysporum f.sp. strigae (Foxy-2) strains as biological control agent (BCA) has shown to be an effective control of the weed Striga hermonthica which is parasitic to several cereals (e.g., maize) cultivated in Sub-Saharan Africa. Most studies have examined the efficacy of the BCA and its interactions with host crops, while overlooking the interplay among key microorganisms in the soil nitrogen (N) cycle. Hence, we postulated that both Foxy-2 and Striga pose threats to the indigenous plant root-associated microbial communities involved in N cycling through direct or indirect competition for nutrients and that the application of high quality organic residues would compensate these effects. The primary objective of this study was thus to assess the potential impact of Foxy-2 on indigenous nitrifying prokaryotes in maize rhizosphere cultivated on two distinct soils (sandy Ferric Alisol versus clayey Humic Nitisol) obtained from Machanga and Embu, respectively, in central Kenya. These soils were treated with or without Foxy-2 and Striga; and in combination with high quality (i.e. CN ratio; 13, lignins, 8.9 % and polyphenols, 1.7 %) organic residues (i.e., Tithonia diversifolia) as N source. Using quantitative polymerase chain reaction (qPCR), we followed at three pre-defined sampling dates (14, 28 and 42 days after planting) the responses of ammonia-oxidizing archaea (AOA) and bacteria (AOB), total bacteria and archaea in four treatments of a rhizobox experiment: (i) Foxy-2 plus Striga (F+S), (ii) Striga only (C+S), (iii) Foxy-2 plus Striga plus Tithonia diversifolia residues (F+S+T), and (iv) a non-treated control (C). Overall, the treatment effects on soil microbial populations were, in comparison to the clayey Embu soil, more pronounced in the sandy Machanga soil. Contrary to our expectations, we observed a distinct stimulative, but no resource competition effect of Foxy-2 on the abundance of AOA, as well as

  18. Características Dendrométricas, Físicas e Químicas da Myracrodruon urundeuva e da Leucaena leucocephala

    Directory of Open Access Journals (Sweden)

    Lázaro Lavoisier Honorato da Silva

    2017-04-01

    Full Text Available RESUMO Com o objetivo de avaliar características dendrométricas, físicas e químicas da Myracrodruon urundeuva e Leucaena leucocephala foi realizada supressão de cinco exemplares de cada espécie. Os parâmetros dendrométricos avaliados foram: DAP, altura, volume. Avaliou-se a densidade básica e foram feitas análises químicas da madeira. O experimento foi arranjado em delineamento inteiramente casualizado (DIC, com valores analisados pelo teste F ao nível de 5%. As espécies M. urundeuva e L. leucocephala apresentaram, respectivamente, média de DAP 10,00 cm; 14,08 cm; altura total 8,20 m; 12,93 m; altura comercial 4,90 m; 10,07 m; volume com casca (C/C 0,032 m3; 0,104 m3; volume sem casca (S/C 0,025 m3; 0,095 m3. M. urundeuva apresentou maior densidade básica. O teor de cinzas encontrado para M. urundeuva foi superior. Já os teores de extrativos totais e holocelulose da M. urundeuva e da L. leucocephala foram semelhantes. A L. leucocephala obteve maior teor de lignina total e poder calorífico superior. Portanto, as duas espécies apresentaram boas características físicas, químicas e energéticas.

  19. Evaluación del establecimiento de Leucaena leucocephala cv. Cunningham en una finca ganadera del municipio de Perico, Matanzas, Cuba

    Directory of Open Access Journals (Sweden)

    Katia Bover

    Full Text Available El objetivo de este trabajo fue evaluar el establecimiento de Leucaena leucocephala cv. Cunningham en una finca de la cooperativa de créditos y servicios (CCS Ramón Rodríguez Milián, del municipio de Perico. Se trabajó en un área de 6 ha, dividida en cinco cuartones, y se realizó una preparación en franjas de 2 m de ancho. Se determinó la supervivencia después del trasplante, la altura, el número de ramas y la composición florística del pastizal; así como el efecto de las labores de limpieza y el daño causado por los animales en la altura. Se aplicó un análisis de varianza y los datos se procesaron con el paquete estadístico SPSS® versión 10.0 para Windows XP. La supervivencia de las plantas fue de 98 %; la labor de limpieza tuvo un efecto positivo en el crecimiento, mientras que la defoliación de la planta por los animales afectó el establecimiento. La población de pastos naturales (jiribilla y pitilla se mantuvo alrededor de 66 % y la de las principales leguminosas volubles (teramnus, glycine y siratro disminuyó de 12 % en el tercer mes después de realizado el trasplante, a 6 % en el decimoquinto mes. El sistema se consideró establecido cuando las plantas sobrepasaron los 2 m de altura. Se concluye que la labor de limpieza influyó positivamente en el comportamiento de la altura y el establecimiento de L. leucocephala, mientras que las defoliaciones causadas por los animales lo retrasaron.

  20. Characterization of chemical elements of fruits of Leucaena leucocephala in riverside areas of the Piracicaba River Basin, São Paulo, Brazil; Caracterização de elementos químicos de frutos de Leucaena leucocephala em áreas ribeirinhas da Bacia do Rio Piracicaba, São Paulo, Brasil

    Energy Technology Data Exchange (ETDEWEB)

    França, Elvis J.; Magalhães, Marcelo R.L. de; Ferreira, Fabiano S., E-mail: ejfranca@cnen.gov.br, E-mail: marcelo_rlm@hotmail.com, E-mail: biologofabiano10@gmail.com [Centro de Ciência Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil); Fernandes, Elisabete A. de N.; Fonseca, Felipe Y.; Rodrigues, Vanessa S.; Cavalca, Isabel P.O.; Camilli, Leandro, E-mail: lis@cena.usp.br, E-mail: vsrodrigues89@gmail.com, E-mail: i-cavalcante@uol.com.br [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil); Silva, Bruno F., E-mail: brunodasilva1996@hotmail.com [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil)

    2017-07-01

    The species Leucaena leucocephala is present in several environments. Its fruit is widely used in cattle feed. However, it is classified as an invasive species, causing problems in the restoration of impacted areas. The Piracicaba River Basin is heavily affected by urbanization and industrialization, which shows the proliferation of this species. Considering the importance of this tree species for Brazil, the present study quantifies chemical elements in fruits of this species by applying Instrumental Neutronic Activation Analysis, method k{sup 0}. Samples were collected in riverside areas of the Piracicaba Municipality, São Paulo, Brazil, at different periods (dry and rainy season). After collection, the samples were shattered and transferred to polyethylene capsules and irradiated with neutrons. Certified reference materials were used to evaluate the quality of the analytical procedure, all material was irradiated under a thermal neutron flux of 10{sup 13} cm-2s-1 for 8 hours. Ni-Cr alloy was used for the monitoring of thermal neutron flux. High Resolution Range Spectrometry with HPG detectors was employed for the measurement of the induced radioactivity, allowing the quantification of the chemical elements. Compared with available results of chemical analysis of pods, the chemical elements Ca, K, Ba, Cr and Se presented high concentrations. We also quantified chemical elements of environmental relevance such as As, Hg, Br, Ce, Cs, Eu, Hf, La, Rb, Sb, Sc, Sm, Ta, Tb, Th and Yb, indicating the need for element dynamics studies in the environments occupied by this invasive species.

  1. SP-100 Program overview

    International Nuclear Information System (INIS)

    Truscello, V.C.

    1984-01-01

    The SP-100 Program is expected to go through three phases: technology assessment and advancement, ground testing, and flight qualification. Currently the program is in the two- to three-year technology assessment and advancement stage. Goals are to identify the space nuclear power system concept that best meets anticipated requirements of future space missions, assess the technical feasibility of that concept, and establish a cost and schedule for developing the concept. The SP-100 Project Office has begun the implementation activities needed to meet these goals. With regard to refractory alloys, a better data base will be required before we move ahead in the program from technology assessment to ground demonstration

  2. Potential of different AM fungi (native from As-contaminated and uncontaminated soils) for supporting Leucaena leucocephala growth in As-contaminated soil.

    Science.gov (United States)

    Schneider, Jerusa; Bundschuh, Jochen; Rangel, Wesley de Melo; Guilherme, Luiz Roberto Guimarães

    2017-05-01

    Arbuscular mycorrhizal (AM) fungi inoculation is considered a potential biotechnological tool for an eco-friendly remediation of hazardous contaminants. However, the mechanisms explaining how AM fungi attenuate the phytotoxicity of metal(oid)s, in particular arsenic (As), are still not fully understood. The influence of As on plant growth and the antioxidant system was studied in Leucaena leucocephala plants inoculated with different isolates of AM fungi and exposed to increasing concentrations of As (0, 35, and 75 mg dm -3 ) in a Typic Quartzipsamment soil. The study was conducted under greenhouse conditions using isolates of AM fungi selected from uncontaminated soils (Acaulospora morrowiae, Rhizophagus clarus, Gigaspora albida; and a mixed inoculum derived from combining these isolates, named AMF Mix) as well as a mix of three isolates from an As-contaminated soil (A. morrowiae, R. clarus, and Paraglomus occultum). After 21 weeks, the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were determined in the shoots in addition to measuring plant height and mineral contents. In general, AM fungi have shown multiple beneficial effects on L. leucocephala growth. Although the activity of most of the stress-related enzymes increased in plants associated with AM fungi, the percentage increase caused by adding As to the soil was even greater for non-mycorrhizal plants when compared to AM-fungi inoculated ones, which highlights the phytoprotective effect provided by the AM symbiosis. The highest P/As ratio observed in AM-fungi plants, compared to non-mycorrhizal ones, can be considered a good indicator that the AM fungi alter the pattern of As(V) uptake from As-contaminated soil. Our results underline the role of AM fungi in increasing the tolerance of L. leucocephala to As stress and emphasize the potential of the symbiosis L. leucocephala-R. clarus for As-phytostabilization at moderately As

  3. CARACTERIZACIÓN DE DIEZ CULTIVARES FORRAJEROS DE Leucaena leucocephala BASADA EN LA COMPOSICIÓN QUÍMICA Y LA DEGRADABILIDAD RUMINAL

    Directory of Open Access Journals (Sweden)

    Danny García M

    2008-08-01

    Full Text Available Objetivo. Estudiar las variaciones en la composición química y la degradabilidad ruminal de diez cultivares de Leucaena leucocephala Lam. de Wit. mediante el análisis de componentes principales (ACP. Materiales y métodos. Se tomaron muestras durante tres años para evaluar la composición química, los niveles de metabolitos secundarios y la degradabilidad ruminal en ovinos. Los datos fueron analizados con el paquete estadístico SPSS y mediante el diagrama tridimensional se obtuvieron las agrupaciones de las accesiones en dependencia de sus características nutritivas. Resultados. Con los primeros tres componentes del ACP se explicó el 85.83 % de la variabilidad. La concentración de proteínas, fracción fibrosa, minerales, polifenoles, fitatos y la degradación ruminal presentaron las mayores fluctuaciones. Las agrupaciones formadas permitieron identificar seis grupos con características químicas diferentes (G1: elevada cantidad de proteínas, baja proporción de fibra y de metabolitos secundarios y elevada degradación -cv. CNIA-250 y cv. K-28-; G-2: elevado contenido de materia seca, proteínas y fenoles, poca fracción de fibra y taninos y baja degradabilidad ruminal -cv. Ipil-Ipil-; G-3: composición nutricional promedio -cv. México-; G-4: bajo contenido de materia seca, fenoles, mimosina y elevada degradación ruminal -cv. Cunningham, cv. 7 y cv. América-; G-5: elevado contenido de materia seca, fenoles y valor nutritivo medio -cv. K-8 y cv. K-67-; G-6: elevado porcentaje de materia seca y taninos, y baja degradabilidad -cv. Perú-. Conclusiones. La biomasa comestible de los cultivares Cunningham, América, 7, México, CNIA-250 y K-28 constituyen las mejores opciones para la alimentación de rumiantes.

  4. Respuesta de Leucaena leucocephala cv. Perú a la aplicación de diferentes dosis de MicoFert agrícola

    Directory of Open Access Journals (Sweden)

    L Ojeda

    Full Text Available Se realizó una investigación en la Empresa Pecuaria El Tablón (Cumanayagua, provincia Cienfuegos, Cuba, con el objetivo de evaluar el efecto que ejerce la aplicación de diferentes dosis de MicoFert agrícola sobre la producción de materia seca (MS y el contenido de fósforo foliar en Leucaena leucocephala cv. Perú. El diseño fue de bloques al azar, con seis tratamientos y tres réplicas. Los tratamientos estuvieron constituidos por cuatro dosis de MicoFert (250, 500, 750 y 1 000 g/m-1, una variante a razón de 25 kg de N ha-1, y el control. El experimento tuvo una duración de dos años; se realizaron cuatro cortes por año, con una frecuencia de 90 días, a una altura de 25 cm sobre el suelo. La aplicación de MicoFert agrícola incrementó el rendimiento de MS entre 13 y 40 %, de forma proporcional a las dosis aplicadas, respecto al control. El rendimiento más alto de MS (18,44 t ha-1 se obtuvo con la aplicación de nitrógeno, y difirió significativamente del resto de los tratamientos. Fue evidente el efecto que ejerció la inoculación con el biofertilizante en el contenido de fósforo foliar, el cual mostró diferencias significativas entre las variantes con MicoFert y el control. La colonización de raicillas por los hongos micorrízicos arbusculares (HMA fue proporcional al incremento de las dosis de biofertilizante, con valores de 34, 38, 44 y 49 %, respectivamente. La fertilización con nitrógeno y el control presentaron 28 y 26 % de colonización micorrízica, lo que indicó la presencia de HMA nativos.

  5. Cryptosporidium sp. in lizards

    Czech Academy of Sciences Publication Activity Database

    Koudela, Břetislav; Modrý, D.

    1998-01-01

    Roč. 45, č. 1 (1998), s. 8 ISSN 1066-5234. [Cryptosporidium sp. in lazards. 01.01.1998-02.01.1998, Praha] R&D Projects: GA ČR GA508/95/0273; GA AV ČR IPP2020702 Subject RIV: fp - Other Medical Disciplines

  6. Cinética de fermentación in vitro de Leucaena leucocephala y Megathyrsus maximus y sus mezclas, con o sin suplementación energética

    Directory of Open Access Journals (Sweden)

    Xiomara Gaviria

    Full Text Available Con el objetivo de caracterizar la cinética de fermentación in vitro de mezclas de Leucaena leucocephala y Megathyrsus maximus, se realizó un experimento in vitro de producción de gas en el que se incluyeron cinco tratamientos: 100 % de L. leucocephala (L100, 100 % de M. maximus (G100, 100 % de suplemento a base de harina de arroz y melaza (S100, y dos proporciones: L23-G77 y L26-G70-S4. Los forrajes se recolectaron durante ocho meses en un sistema silvopastoril intensivo (SSPi, perteneciente al Centro Agropecuario Cotové de la Universidad Nacional de Colombia. La producción máxima de gas varió en un rango de 156 (L100 a 247 mL g-1 sustrato (L26-G70-S4. El menor volumen de gas al punto de inflexión (57,5 mL se observó en L100, el cual fue diferente al de las mezclas y el suplemento (p < 0,05. La desaparición de la MS a las 96 h varió entre 53,8 % y 66,9 %, y fue mayor en L100 que en el resto de los tratamientos (p < 0,05. El menor valor de la producción de gas (1,31 mL por cada gramo de MS, fermentada a las 96 h, se observó en L100 (p < 0,05. Los resultados sugieren que la inclusión de leucaena aumentó la concentración de proteína de la dieta y redujo el contenido de FDN, lo que resulta positivo desde el punto de vista de la productividad animal. Se concluye que la utilización de forrajes de mayor calidad nutricional, como el de leucaena, modifica el perfil de fermentación de la dieta; por lo que la respuesta de las mezclas forraje-gramínea es diferente a la esperada, ya que depende de la respuesta individual de cada forraje.

  7. Evaluación de dos especies de Leucaena, asociadas a Brachiaria brizantha y Clitoria ternatea en un sistema silvopastoril de Nayarit, México: I. Comportamiento agronómico Evaluation of two Leucaena species, associated to Brachiaria brizantha and Clitoria ternatea in a silvopastoral system from Nayarit, Mexico: I. Agronomic performance

    Directory of Open Access Journals (Sweden)

    J Bugarín

    2009-12-01

    Full Text Available Con el objetivo de evaluar el comportamiento agronómico de dos especies de Leucaena, asociadas a Brachiaria brizantha y Clitoria ternatea en un sistema silvopastoril en la llanura costera norte del estado de Nayarit, en México, se desarrolló la presente investigación. Se utilizó un diseño de bloques al azar con cuatro repeticiones por tratamiento (proporción de leguminosa:gramínea: 1 L. leucocephala-B. brizantha (30:70; 2 L. glauca-B. brizantha (30:70; 3 L. leucocephala-B. brizantha- C. ternatea (28:52:20; 4 L. glauca-B. brizantha-C. ternatea (28:52:20; 5 B. brizantha (100. Con una frecuencia mensual se midió el crecimiento de las especies arbóreas, además de la supervivencia y el comportamiento de la relación leguminosa:gramínea en el sistema. L. glauca mostró una mayor altura (66 cm y diámetro (0,98 cm cuando se combinó con brachiaria y clitoria, y L. leucocephala un mayor número de ramas (8,7 y altura (4,5 cm. En la sobrevivencia se observaron valores de 70 a 80% después de un año de implementado el sistema. De acuerdo con los resultados se concluye que la mezcla de las leguminosas arbóreas con C. ternatea y B. brizantha es una importante opción en las condiciones ecológicas del trópico seco mexicano.This study was conducted with the objective of evaluating the agronomic performance of two Leucaena species, associated to Brachiaria brizantha and Clitoria ternatea in a silvopastoral system in the northern coastal prairie of the Nayarit state, Mexico. A randomized block design was used with four repetitions per treatment (legume:grass proportion: 1 L. leucocephala-B. brizantha (30:70; 2 L. glauca-B. brizantha (30:70; 3 L. leucocephala-B. brizantha- C. ternatea (28:52:20; 4 L. glauca-B. brizantha-C. ternatea (28:52:20; 5 B. brizantha (100. Tree growth was measured on a monthly basis, in addition to survival and the performance of the legume:grass relationship in the system. L. glauca showed more height (66 cm and diameter

  8. Desempenho germinativo da invasora Leucaena leucocephala (Lam. de Wit. e comparação com Caesalpinia ferrea Mart. ex Tul. e Caesalpinia pulcherrima (L. Sw. (Fabaceae Germination performance of the invader Leucaena leucocephala (Lam. de Wit. compared to Caesalpinia ferrea Mart. ex Tul. and C. pulcherrima (L. Sw. (Fabaceae

    Directory of Open Access Journals (Sweden)

    Nilson Gonçalves da Fonseca

    2011-03-01

    Full Text Available O sucesso das plantas na colonização de novas áreas está fortemente relacionado ao comportamento germinativo das sementes nas condições ambientais locais. O objetivo deste trabalho foi comparar o desempenho germinativo da exótica invasora Leucaena leucocephala ao das leguminosas (Caesalpinia ferrea, nativa e C. pulcherrima, exótica não consideradas invasoras. Sementes não escarificadas foram expostas às temperaturas de 15, 20, 25, 30, 35 e 40 °C, sob um fotoperíodo de 12 horas, durante 100 dias. Tanto a porcentagem final quanto a velocidade de germinação apresentaram diferenças significativas entre as três espécies. C. ferrea teve baixo desempenho, com temperatura ótima para a germinação a 25°C, porcentagem de germinação de 59% e IVG de 0,68 sementes d-1. Já C. pulcherrima, apresentou altas porcentagens de germinação em todas as temperaturas testadas, com faixa ótima entre 20-40 °C. As sementes de L. leucocephala apresentaram as menores porcentagens de germinação e temperatura ótima de 35 °C. O desempenho germinativo desta exótica não se sobressai ao das demais espécies estudadas e fatores como a pressão de propágulos, escarificação natural e bom desempenho em outras fases da vida devem contribuir para seu perfil de invasora.Success in establishing and colonizing new areas is directly related to germination behaviour of seeds under local environmental conditions. This work aimed to compare germination performance of the exotic invader Leucaena leucocephala to two legumes (Caesalpinia ferrea, native, and C. pulcherrima, exotic which are not considered invasive. Non-scarified seeds were exposed to constant temperatures of 15, 20, 25, 30, 35 and 40ºC, within a photoperiod of 12 hours, for 100 days. Both final germination percentage and germination speed showed significant differences among the three species. C. ferrea had low germination percentage, with optimal germination temperature at 25ºC, reaching

  9. Descomposición de la hojarasca en un sistema silvopastoril de Panicum maximum y Leucaena leucocephala (Lam de Wit cv. Cunningham: III. Influencia de la densidad y diversidad de la macrofauna asociada Litter decomposition in a silvopastoral system of Panicum maximum and Leucaena leucocephala (Lam de Wit cv. Cunningham: III. Influence of density and diversity of the associated macrofauna

    Directory of Open Access Journals (Sweden)

    Saray Sánchez

    2010-03-01

    Full Text Available Con el objetivo de determinar la descomposición de la hojarasca en un sistema silvopastoril de Panicum maximum y Leucaena leucocephala y su relación con la densidad y la diversidad de la macrofauna asociada, se realizó este experimento en la EEPF «Indio Hatuey». Esta se determinó como la pérdida de biomasa a través del tiempo, con relación al peso inicial. Para el estudio de la dinámica de la descomposición se utilizó el método de bolsas de hojarasca (litter bags. Se escogieron al azar cuatro bolsas a los 30, 60, 90, 120, 150, 180 y 210 días de situadas en el pastizal. En cada fecha de recolección, a la hojarasca remanente de cada bolsa se le determinó la población de macrofauna (organismos con diámetro mayor que 2 mm mediante la separación manual, y se calculó el valor promedio de la densidad (individuos/m², así como la abundancia proporcional (% para cada taxón. Se utilizó el análisis de correlación y regresión para conocer la interrelación entre las variables y los modelos de mejor ajuste. De acuerdo con los resultados se concluye que las condiciones de humedad y temperatura que genera el árbol en este sistema, así como la calidad de su hojarasca, posibilitan la presencia de una diversa y estable fauna asociada a las bolsas, la cual influyó en el proceso de descomposición.The trial was conducted at the EEPF «Indio Hatuey» in order to determine the litter decomposition in a silvopastoral system of Panicum maximum and Leucaena leucocephala and its relation to the density and diversity of the associated macrofauna. It was determined as biomass loss through time, with regards to initial weight. For the study of the decomposition dynamics the litter bag method was used. Four bags were randomly chosen 30, 60, 90, 120, 150, 180 and 210 days after being placed in the pastureland. In each collection date, to the remnant litter of each bag, the macrofauna (organisms with diameter higher than 2 mm population was

  10. Efeito da suplementação com feno de Leucena (Leucaena leucocephala (Lam de Wit durante a estação seca sobre o desenvolvimento ponderal de ovinos Effect of supplementation with Leucaena leucocephala hay during the dry season on the ponderal development sheep

    Directory of Open Access Journals (Sweden)

    Antônio Alves de Souza

    1999-01-01

    Full Text Available O objetivo deste experimento foi estudar o desempenho de borregos suplementados com dois níveis de feno de leucena (Leucaena leucocephala (Lam de Wit, durante a estação seca, em comparação com animais mantidos em pastagem exclusiva de capim-buffel (Cenchrus ciliaris, L ou em pastagem consorciada de capim-buffel com guandu (Cajanus cajan. Durante a estação das águas, foi determinado ainda o desempenho dos animais que foram mantidos em pastagem comum de capim-buffel, sem suplementação. Os seguintes tratamentos foram testados durante a estação seca: A - pasto de capim-buffel; B - pasto de capim-buffel consorciado com guandu; C - pasto de capim-buffel suplementado com feno de leucena (250 g/animal•d; e D - pasto de capim-buffel suplementado com feno de leucena (500 g/animal•d. Durante a estação seca, somente os borregos suplementados com o nível mais alto de feno de leucena (tratamento D apresentaram ganho diário de peso mais elevado que os animais mantidos em dieta exclusiva de pasto (tratamentos A e B. Na estação das águas, os animais que apresentaram melhor desempenho durante a estação seca passaram a apresentar ganho diário de peso inferior em relação aos dos demais tratamentos. Quando as duas estações foram consideradas em conjunto, a vantagem com a suplementação desapareceu e não houve diferenças entre tratamentos. O uso de feno de leucena para suplementação de borregos mantidos a pasto, durante a estação seca, é eficiente, desde que os animais estejam terminados e sejam abatidos ao final da estação.

  11. Siembra directa con Leucaena leucocephala (Lam. de Wit. Para producción de forraje en el jardín botánico “Carlos Liscano”, Mérida, Venezuela

    Directory of Open Access Journals (Sweden)

    J. Petit-Aldana

    2005-01-01

    Full Text Available La siembra directa es un método económico para establecer programas de producción de forraje con leguminosas arbustivas, La Leucaena leucocephala (Lam. de Wit. es una leguminosa de tipo arbustivo, utilizada en agricultura, ganadería y en la recuperación de tierras, tiene gran adaptabilidad, alto valor forrajero y se cataloga entre las principales especies de uso múltiple en agroforestería. El objetivo fue establecer un ensayo de siembra directa utilizando los métodos de siembra al voleo, surcos y hoyos con Leucaena leucocephala (Lam. de Wit en un área del Jardín Botánico “Carlos Liscano” de la Facultad de Ciencias Forestales y Ambientales de la Universidad de Los Andes, Venezuela, a fin de probar su efectividad para planes futuros de establecimiento de bancos forrajeros. Se estableció el ensayo en un diseño completamente al azar en un experimento factorial (2 x 3, con 4 bloques (repeticiones. Los factores experimentales fueron: Factor A: 1 terreno arado 2 terreno no arado y Factor B: 1 siembra al voleo, 2 siembra en hoyos y 3 siembra en surcos. Se encontró que el método más exitoso de siembra es el surco, tanto en terreno arado como no arado. Se determinó que la emergencia está entre el día cuatro y dieciocho después de la siembra. Tres meses después la mayor abundancia de plantas se presentó en los bloques arados con sistema de siembra al voleo, aunque los mejores promedios de altura se encontraron en los bloques con arado y la siembra en surcos.

  12. Evaluación de dos especies de Leucaena, asociadas a Brachiaria brizantha y Clitoria ternatea en un sistema silvopastoril de Nayarit, México: II. Producción y composición bromatológica de la biomasa Evaluation of two Leucaena species, associated to Brachiaria brizantha and Clitoria ternatea in a silvopastoral system from Nayarit, Mexico: II. Biomass production and bromatological composition

    Directory of Open Access Journals (Sweden)

    J Bugarín

    2009-12-01

    Full Text Available Con el objetivo de caracterizar la producción y la calidad nutricional de la biomasa en un sistema silvopastoril de la llanura costera norte del estado de Nayarit, México, se desarrolló la presente investigación. Los tratamientos fueron: 1 Leucaena leucocephala-B. brizantha (30:70; 2 Leucaena glauca-B. brizantha (30:70; 3 L. leucocephala-B. brizantha-C. ternatea (28:52:20; 4 L. glauca-B. brizantha-C. ternatea (28:52:20; 5 B. brizantha (100. Se estimó la producción de biomasa y se determinó: materia seca, cenizas, materia orgánica, proteína bruta, hemicelulosa y las fracciones de fibra ácido y neutro detergente. Los valores más bajos en cuanto a la producción de biomasa, a los seis meses de establecido el sistema, fueron para el testigo (0,78 t/ha, con diferencias significativas. A su vez, la combinación de las tres especies aportó los mejores resultados (1,64 y 1,69 t/ha para los tratamientos 3 y 4, respectivamente y a los nueve meses triplicaron la producción de biomasa. Hubo diferencias significativas en la composición bromatológica en todas las combinaciones evaluadas. No obstante, todas las especies mostraron características nutricionales adecuadas y constituyen una alternativa para los sistemas silvopastoriles tropicales en esta región.This study was conducted with the objective of characterizing the biomass production and nutritional quality in a silvopastoral system of the northern coastal plain of the Nayarit state, Mexico. The treatments were: 1 Leucaena leucocephala-B. brizantha (30:70; 2 Leucaena glauca-B. brizantha (30:70; 3 L. leucocephala-B. brizantha- C. ternatea (28:52:20; 4 L. glauca-B. brizantha-C. ternatea (28:52:20; 5 B. brizantha (100. The biomass production was estimated and the following indicators were determined: dry matter, ashes, organic matter, crude protein, hemicellulose and the fractions of acid and neutral detergent fiber. The lowest values regarding biomass production, six months after

  13. Control of Branchionus sp. and Amoeba sp. in cultures of Arthrospira sp. Control de Branchionus sp. y Amoeba sp. en cultivos de Arthrospira sp.

    Directory of Open Access Journals (Sweden)

    Carlos Méndez

    2012-09-01

    Full Text Available Cultivation of cyanobacterium Arthrospira sp. has been developed in many countries for the production of proteins, pigments and other compounds. Outdoor mass cultures are often affected by biological contamination, drastically reducing productivity as far as bringing death. This study evaluates the control of Branchionus sp. and Amoeba sp. with two chemical compounds: urea (U and ammonium bicarbonate (AB, in laboratory conditions and outdoor mass culture of Arthrospira sp. The lethal concentration 100 (LC100 at 24 h for Branchionus sp. and Amoeba sp. determined was of 60-80 mg L-1 (U and 100-150 mg L-1 (AB. The average effective inhibition concentration for 50% of the population (IC50 in Arthrospira sp., after 72 h, was 80 mg L-1 (U and 150 mg L-1 (AB. The application of doses of 60 mg L-1 (U or 100 mg L-1 (AB in the outdoor mass culture of this contaminated microalga, completely inhibited grazing and did not affect the growth of Arthrospira sp. but rather promoted rapid recovery of algal density at levels prior to infestation. These compounds provided an economical and effective control of predators in cultures of Arthrospira sp.El cultivo de la cianobacteria Arthrospira sp. ha sido desarrollado en muchos países para la obtención de proteínas, pigmentos y otros compuestos. Cultivo que a nivel industrial se ve afectado frecuentemente por contaminación biológica, reduciendo drásticamente la productividad hasta causar la muerte. Este estudio evalúa el control de Branchionus sp. y de Amoeba sp. con dos compuestos químicos, la urea (U y bicarbonato de amonio (AB en cultivos de Arthrospira sp. La concentración letal 100 (LC100 determinada a las 24 h para Branchionus sp. y Amoeba sp. fue de 60-80 mg L-1 (U y 100-150 mg L-1 (AB. La concentración media de inhibición efectiva, después de 72 h, para el 50% de la población (IC50 en Arthrospira fue de 80 mg L-1 (U y 150 mg L-1 (AB. La aplicación de dosis de 60 mg L-1 (U ó 100 mg L-1 (AB en

  14. SP mountain data analysis

    Science.gov (United States)

    Rawson, R. F.; Hamilton, R. E.; Liskow, C. L.; Dias, A. R.; Jackson, P. L.

    1981-01-01

    An analysis of synthetic aperture radar data of SP Mountain was undertaken to demonstrate the use of digital image processing techniques to aid in geologic interpretation of SAR data. These data were collected with the ERIM X- and L-band airborne SAR using like- and cross-polarizations. The resulting signal films were used to produce computer compatible tapes, from which four-channel imagery was generated. Slant range-to-ground range and range-azimuth-scale corrections were made in order to facilitate image registration; intensity corrections were also made. Manual interpretation of the imagery showed that L-band represented the geology of the area better than X-band. Several differences between the various images were also noted. Further digital analysis of the corrected data was done for enhancement purposes. This analysis included application of an MSS differencing routine and development of a routine for removal of relief displacement. It was found that accurate registration of the SAR channels is critical to the effectiveness of the differencing routine. Use of the relief displacement algorithm on the SP Mountain data demonstrated the feasibility of the technique.

  15. Consideraciones acerca de la Leucaena leucocephala cv. X: una nueva opción forrajera para un ecosistema ganadero con suelos ácidos e infértiles Considerations about Leucaena leucocephala cv. X: A new forage choice for a livestock ecosystem with acid and infertile soils

    Directory of Open Access Journals (Sweden)

    A Pérez

    2008-12-01

    Full Text Available El presente estudio se realizó a partir de una búsqueda de información por diversas páginas Web, revistas de impacto, otras de carácter nacional e internacional, así como las más diversas fuentes de información, relacionada con la capacidad de adaptación del género Leucaena a los diferentes tipos de suelo, con énfasis en la acidez y el grado de encharcamiento. La revisión proporcionó las limitantes de adaptación del género y de la especie L. leucocephala a dichas condiciones. El descubrimiento de las posibilidades de germoplasma adaptables a esos tipos de suelos, abrió un preámbulo para encontrar plantas arbóreas para los ecosistemas con acidez y humedad, de los que Cuba y el Trópico poseen más del 33% en las áreas cultivadas. Se brinda información internacional, así como resultados de investigación acerca de variedades de esta especie, la fase de establecimiento, la fenología de la planta, la adaptación a diferentes tipos de suelo, la respuesta a la poda y el comportamiento con animales, fundamentalmente en la producción de leche, como respuesta al consumo y suministro de L. leucocephala cv. X. Los resultados permiten afirmar que el cv. X, junto a otras gramíneas y leguminosas importantes para este tipo de ecosistema ganadero, puede contribuir significativamente a transformar el panorama económico, productivo y ambiental del entorno de las zonas que presenten similares condiciones. Se concluye que esta leguminosa arbórea puede ser un buen árbol forrajero para suelos ácidos (pH entre 4,6 y 5,1, húmedos e infértiles, lo que le confiere una plasticidad ecológica superior a la considerada hasta el momento. Se recomienda que este cultivar continúe extendiéndose a otras zonas con condiciones similares a las estudiadas. Además, debe continuarse su evaluación para proponerla como variedad comercial.The study was carried out from a search for information through different Web pages, impact journals, other national

  16. SP-100 Test Site

    International Nuclear Information System (INIS)

    Cox, C.M.; Mahaffey, M.K.; Miller, W.C.

    1988-01-01

    Preparatory activities are well under way at Hanford to convert the 309 Containment Building and its associated service wing to a 2.5 MWt nuclear test facility for the SP-100 Ground Engineering System (GES) test. Preliminary design is complete, encompassing facility modifications, a secondary heat transport system, a large vacuum system to enclose the high temperature reactor, a test assembly cell and handling system, control and data processing systems, and safety and auxiliary systems. The design makes extensive use of existing equipment to minimize technical risk and cost. Refurbishment of this equipment is 75% complete. The facility has been cleared of obstructing equipment from its earlier reactor test. Current activities are focusing on definitive design and preparation of the Preliminary Safety Analysis Report (PSAR) aimed at procurement and construction approvals and schedules to achieve reactor criticality by January 1992. 6 refs

  17. Bradysia sp. em morangueiro Bradysia sp. in strawberry

    Directory of Open Access Journals (Sweden)

    Bernadete Radin

    2009-04-01

    Full Text Available No trabalho, relatam-se os primeiros registros de Bradysia sp. (Insecta: Diptera: Sciaridae em morangueiro (Fragaria x ananassa Duch., cultivado no Município de Eldorado do Sul, RS. O cultivo foi realizado em sacolas com três metros de comprimento, preenchidas com substrato composto de casca de arroz e turfa, dispostas horizontalmente sobre bancadas de madeira, em ambiente protegido. A presença de Bradysia sp. foi observada na segunda quinzena de agosto de 2005. Neste trabalho, estão descritos os sintomas apresentados no morangueiro pela praga, prováveis conseqüências sobre o aparecimento de doenças e uma breve descrição morfológica da Bradysia sp., adulto e fase larval.This paper describes the first record of Bradysia sp. (Insecta; Diptera; Sciaridae in strawberry (Fragaria x ananassa, cultivated in the city of Eldorado do Sul, RS, Brazil. Strawberry was planted in plastic bags filled with a mixture of burnt rice hulls and peat and cultivated in a greenhouse. The presence of Bradysia sp was noticed in the second fortnight of August, 2005. The symptoms in strawberry and the probable consequences in terms of disease arising were described in the present study, as well as the morphological characterization of Bradysia sp. and its illustrations.

  18. Yersinia pekkanenii sp. nov.

    Science.gov (United States)

    Murros-Kontiainen, Anna; Johansson, Per; Niskanen, Taina; Fredriksson-Ahomaa, Maria; Korkeala, Hannu; Björkroth, Johanna

    2011-10-01

    The taxonomic position of three strains from water, soil and lettuce samples was studied by using a polyphasic taxonomic approach. The strains were reported to lack the virulence-encoding genes inv and virF in a previous study. Controversially, API 20 E and some other phenotypic tests suggested that the strains belong to Yersinia pseudotuberculosis, which prompted this polyphasic taxonomic study. In both the phylogenetic analyses of four housekeeping genes (glnA, gyrB, recA and HSP60) and numerical analyses of HindIII and EcoRI ribopatterns, the strains formed a separate group within the genus Yersinia. Analysis of the 16S rRNA gene sequences showed that the strains were related to Yersinia aldovae and Yersinia mollaretii, but DNA-DNA hybridization analysis differentiated them from these species. Based on the results of the phylogenetic and DNA-DNA hybridization analyses, a novel species, Yersinia pekkanenii sp. nov., is proposed. The type strain is ÅYV7.1KOH2(T) ( = DSM 22769(T)  = LMG 25369(T)).

  19. Caracterización cultural y morfológica e identificación de diez aislamientos de Fusarium procedentes de semillas de Leucaena leucocephala cv. Perú almacenadas Cultural and morphological characterization and identification of ten Fusarium isolates from stored Leucaena leucocephala cv. Peru seeds

    Directory of Open Access Journals (Sweden)

    J. C Lezcano

    2012-06-01

    Full Text Available El objetivo de este trabajo fue caracterizar cultural y morfológicamente diez aislamientos de Fusarium, obtenidos de semillas de L. leucocephala cv. Perú almacenadas, con vistas a su clasificación taxonómica. Sus cultivos puros se conservaron en tubos Eppendorf con medio Agar Papa y Dextrosa (APD, en cámara fría a 10°C. La siembra de cada aislamiento se realizó en tres placas Petri, esterilizadas, de 9 cm de diámetro; estas contenían, separadamente, los medios APD y Agar Papa Sacarosa (APS, que constituyeron las réplicas; se colocó en su centro un disco micelial de 7 mm de diámetro, procedente de cultivos con 10-15 días de edad, sembrados también en dichos medios. Posteriormente, las placas se sellaron con papel parafinado y se incubaron durante diez días a 25°C, con alternancia de 8 h de luz/16 h de oscuridad, o a oscuridad constante. Las variables culturales medidas fueron: el color de la colonia en el anverso y el reverso, la textura y el crecimiento lineal y aéreo; y como morfológicas se evaluaron: el tamaño, la forma y el color de las estructuras vegetativas y reproductivas que aparecieron en los cultivos. Se utilizó un diseño completamente aleatorizado y los datos se procesaron estadísticamente a través de un análisis descriptivo. Para la identificación de los hongos se utilizaron diferentes claves taxonómicas. Los aislamientos 10, 18 y 29 se correspondieron con las características descritas en la literatura científica para la especie Fusarium oxysporum Schlecht; el 17, el 28 y el 30, con Fusarium sambucinum Fuckel; el 27, con Fusarium semitectum Berk & Rav.; el 25 y el 26, con Fusarium incarnatum (Rob Sacc, y el 31, con Fusarium chlamydosporum Wollenw & Reinking, lo que permitió la identificación de cinco especies de Fusarium asociadas a las simientes almacenadas.The objective of this work was to characterize cultural and morphologically ten Fusarium isolates, obtained from stored Leucaena leucocephala cv

  20. Antimicrobial peptides from Capsicum sp.

    African Journals Online (AJOL)

    ajl yemi

    2011-12-30

    Dec 30, 2011 ... Key words: Antimicrobial peptides, Capsicum sp, Capsicum chinense, chili pepper, agronomical options, ..... of this human activity is resumed by the simple phrase: produce .... It will be interesting to scale the AMPs extraction.

  1. Effet de Leucaena leucocephala, des fientes de volaille ou du fumier de bovins sur la productivité du maïs cultivé sur "terre de barre" au sud Bénin

    Directory of Open Access Journals (Sweden)

    Mallouhi, N.

    1997-01-01

    Full Text Available Effect of Leucaena leucocephala, Poultry Manure or Cattle Manure on Maize Productivity on Feralitic Soil "terre de barre" in South Benin. Our work has consisted on the study of post-effect of Leucaen's prunings and poultry manure buried last year and also on the study of the direct effect of cattle manure and its interaction with Leucaen's pruning on maize productivity on feralitic soil "terre de barre". - The mixture of Leucaen's prunings and poultry manure had positive post-effect giving a yield of fresh ears and grains significantly higher (9 % to the reference and other treatments in the plots over the alley. - The supply of 5 t/ha of cattle manure appears insufficient. On the other hand the mixture of Leucaen's prunings and cattle manure gave the best results related to the soil's properties such as organic matter's content or total exchange capacity of the soil and also concerning the yields. In this case we notice that ail the results are significantly higher to the other treatments. These results emphasize the importance of a supply of organic matter's to the soil thereby fertilizing it in humus and mineral elements.

  2. Leucaena leucocephala pod seed protein as an alternate to animal protein in fish feed and evaluation of its role to fight against infection caused by Vibrio harveyi and Pseudomonas aeruginosa.

    Science.gov (United States)

    Verma, Vipin Kumar; Rani, Kumari Vandana; Kumar, Shiva Raj; Prakash, Om

    2018-05-01

    The laboratory acclimatized Clarias gariepinus (80 ± 10 g) were divided into six groups and five subgroups each containing 10 fish. A fish feed was reconstituted by adding 33% powder of Leucaena leucocephala seed in place of fish trash. Group B, C and E were fed on reconstituted feed and group A, D and F were fed on artificial feed containing animal protein for 7 days prior to start of experiments. Then Group B was challenged with BSA while other groups were challenged with Vibrio harveyi (Group C, D) and Pseudomonas aeruginosa (Group E, F). Group A was used as negative control (not challenged with antigen). The fish were challenged on weekly intervals till 28th day. Blood was collected from one subgroup of each group on day 7, 14, 21 & 28 and finally sacrificed on day 35. Change in body weight, liver function tests (SGOT, SGPT) and serum ALP levels were monitored. The phagocytic index, percentage phagocytosis and nitric oxide levels were measured in macrophages isolated from spleen and head kidney. The levels of total fish immunoglobulin were also measured following indirect ELISA. The results showed improved immune response in fish fed on 33% L. leucocephala pod seed reconstituted feed; however their specific growth rate was low. Copyright © 2018 Elsevier Ltd. All rights reserved.

  3. Modelos de crescimento resultantes da combinação e variações dos modelos de Chapman-Richards e Silva-Bailey aplicados em Leucaena leucocephala (Lam. de Wit.

    Directory of Open Access Journals (Sweden)

    Cícero Carlos Ramos de Brito

    2010-08-01

    Full Text Available O objetivo deste trabalho foi desenvolver novos modelos de crescimento para recursos florestais aplicados à leucena [Leucaena leucocephala (Lam. de Wit], tendo como base as hipóteses biológicas propostas por Chapman-Richards e Silva-Bailey. O experimento de leucena foi conduzido na Estação Experimental da Empresa Pernambucana de Pesquisa Agropecuária - IPA, Caruaru, PE. Foram utilizadas 544 árvores de leucena de um experimento com vinte remedições realizadas ao longo de 12 anos. Compararam-se novos modelos de crescimento resultantes da combinação e variações dos modelos de Chapman-Richards e Silva-Bailey, bem como outros comumente usados em recursos florestais. Para a seleção das equações, utilizaram-se o Índice de Ajuste (IA, o erro-padrão da estimativa e a distribuição gráfica dos resíduos. Os resultados indicaram que todos os modelos testados se ajustaram de maneira satisfatória aos dados, podendo ser utilizados para se estimar o crescimento em altura da leucena.

  4. Seroprevalencia de Leptospira sp., Rickettsia sp. Ehrlichia sp. en trabajadores rurales del departamento de Sucre, Colombia Seroprevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp. in rural workers of Sucre, Colombia

    Directory of Open Access Journals (Sweden)

    Rodrigo Ríos

    2008-06-01

    Full Text Available Objective. Determinar la seroprevalencia de Leptospira sp., Rickettsia sp. y Ehrlichia sp. en trabajadores de áreas rurales del departamento de Sucre. Material y métodos. Se realizó un estudio escriptivo, prospectivo, de corte transversal, que pretendió determinar la seroprevalencia e Leptospira sp., Rickettsia sp. y Ehrlichia sp. en 90 trabajadores de áreas rurales del departamento de Sucre. Se estableció la presencia de anticuerpos séricos anti-IgM específicos anti-Leptospira por la técnica de ELISA indirecta. Para la determinación de Rickettsia sp. y Ehrlichia sp. se uso la técnica de inmunofluorescencia indirecta. Resultados. La población evaluada estaba compuesta por 27 (30% ordeñadores, 21 (23% jornaleros, 18 (20% profesionales del campo y 24 (27% que realizaban otras actividades. Ventidós (24% muestras resultaron positivas en alguna de las pruebas. De éstas, 12 (13,3% fueron positivas para Leptospira sp., 7 (7,8% para Rickettsia sp. y 3 (3,3% ara Ehrlichia sp. Conclusión. Este fue el primer estudio que se llevó a cabo en el departamento de Sucre y permitió demostrar que existe una prevalencia importante de Leptospira p.,Rickettsia sp. y Ehrlichia sp.. Los factores de riesgo ocupacional fueron factores determinantes en la seropositividad.Objective. To determine the seroprevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp. in agricultural workers of Sucre. Methods. A descriptive prospective cross-sectional study was conducted in ninety rural workers of Sucre. Presence of serum antibodies anti-IgM specific anti-Leptospira by indirect ELISA was established. For the determination of Rickettsia and Ehrlichia indirect inmunoflorescence was used. Results.The population was composed by 27 (30% milkers, 21 (23% day workers, 18 farm professionals (20% and 24 (26% workers in others activities. A total of 22 (24% samples were positive to some test. Twelve (13.3% were positive to Leptospira sp., seven (7.8% to Rickettsia sp

  5. SP-100 reactor cell activation

    International Nuclear Information System (INIS)

    Wilcox, A.D.

    1991-09-01

    There are plans to test the SP-100 space reactor for 2 yr in the test facility shown in Figure 1. The vacuum vessel will be in the reactor experiment (RX) cell surrounded by an inert gas atmosphere. It is proposed that the reactor test cell could contain removable-water- shielding tanks to reduce the residual activation dose rates in the test cell after the tests are completed. This reduction will allow the facility to be considered for other uses after the SP-100 tests are completed. The radiation dose rates in the test cell were calculated for several configurations of water-shielding tanks to help evaluate this concept

  6. Fixação do N2 em leucena (Leucaena leucocephala em solo da região semi-árida brasileira submetido à salinização

    Directory of Open Access Journals (Sweden)

    F. G. Carvalho

    1999-06-01

    Full Text Available Com a finalidade de avaliar a tolerância de rhizobium loti em Leucaena leucocephala cv K8, submetida a níveis crescentes de salinidade, realizou-se um experimento em casa de vegetação, usando-se um Podzólico Vermelho-Amarelo textura franco-arenosa, coletado em Serra Talhada (PE. O experimento obedeceu ao arranjo fatorial 5 x 4, no delineamento em blocos ao acaso, com 3 repetições. Foram usados 5 níveis de salinidade do solo (C.E. = 1,5; 6,6; 10,1; 12,8 e 14,4 dS m-1 e 2 tratamentos inoculados (NFB 494 e SEMIA 6069, fertilização nitrogenada com 200 mg kg-1 de N (NH4NO3, e o controle sem inoculação e sem adubação nitrogenada. O incremento dos níveis crescentes de salinidade reduziu a nodulação (número e massa de nódulos, o rendimento de matéria seca e a acumulação de N na parte aérea. O tratamento com fertilização nitrogenada inibiu totalmente a nodulação em leucena, em todos os níveis de salinidade; contudo, promoveu melhores rendimentos na acumulação de N e na produção de matéria seca. Observou-se resposta significativa das plantas inoculadas com a estirpe SEMIA 6069, em todas as características avaliadas, superando os resultados obtidos com o isolado NFB 494.

  7. Costos y beneficios de un sistema silvopastoril intensivo (sspi, con base en Leucaena leucocephala (Estudio de caso en el municipio de Tepalcatepec, Michoacán, México

    Directory of Open Access Journals (Sweden)

    J. M. González

    2013-01-01

    Full Text Available Se analizaron los costos en que se incurren y los beneficios económicos que se generan en un SSPi con base en Leucaena leucocephala, en terrenos de riego por gravedad, con el fin de evaluar la rentabilidad económica de las inversiones y hacer una comparación entre el SSPi y el sistema que tradicionalmente operaba o línea base (40% monocultivo de pasto Tanzania Panicum maximum, 30% de sorgo forrajero Sorghum vulgar bajo el sistema de corte y 30% de agostadero con pastos nativos. Complementando la alimentación del ganado suizo de doble propósito en el SSPi con pulido de arroz a razón de 1.50 kg diarios por vaca durante la ordeña; y en la línea base se utilizó 3.50 kg de alimento comercial diario por vaca ordeñada. Resultando, para el SSPi, una TIR del 13.30% y el VAN al 10%, un saldo positivo de $2’202,170 (pesos mexicanos y en la situación inicial o línea base se obtuvo una TIR del 0.70% y el VAN al 10% resultó negativo, con $-4’717,022 (pesos mexicanos. El SSPi incrementó de manera importante la productividad y la rentabilidad del rancho haciéndolo lucrativo, ya que con el sistema tradicional no era rentable económicamente.

  8. DESCOMPOSICIÓN Y LIBERACIÓN DE NITRÓGENO Y MATERIA ORGÁNICA EN HOJAS DE Leucaena leucocephala (Lam. de Wit, Guazuma ulmifolia Lam. Y Moringa oleifera Lam. EN UN BANCO MIXTO DE FORRAJE

    Directory of Open Access Journals (Sweden)

    Judith Petit-Aldana

    2012-01-01

    Full Text Available Se evaluaron las tasas de descomposición, la liberación de N y MO en hojas de Leucaena leucocephala (Lam. de Wit, Guazuma ulmifolia Lam. y Moringa oleifera Lam. en un banco mixto de forraje en Yucatán, México, en las épocas de lluvias y sequía, en un diseño de bloques completos al azar, cinco tratamientos y cuatro repeticiones. Se llenaron 120 bolsas con hojas frescas de las especies colectadas, y se colocaron en cada unidad experimental. Se evaluaron tres periodos de incubación para cada tratamiento: cuatro, ocho, y 16 semanas por cada época. A finales del experimento se observó que el proceso de descomposición fue más rápido en la temporada de lluvias. La pérdida de peso y la liberación de N y MO fueron más aceleradas en M. oleifera en monocultivo y en la asociación L. leucocephala con M. oleifera. Los patrones de descomposición de todas las especies siguieron el modelo exponencial simple, con más del 80 % de la masa inicial incorporada al final del periodo de estudio. Se concluye que L. leucocephala, G. ulmifolia y M. oleifera en monocultivo y asociadas, demostraron su capacidad para producir considerables cantidades de materia orgánica y liberar importantes cantidades de N en cortos periodos.

  9. Exo-oligosaccharides of Rhizobium sp. strain NGR234 are required for symbiosis with various legumes.

    Science.gov (United States)

    Staehelin, Christian; Forsberg, Lennart S; D'Haeze, Wim; Gao, Mu-Yun; Carlson, Russell W; Xie, Zhi-Ping; Pellock, Brett J; Jones, Kathryn M; Walker, Graham C; Streit, Wolfgang R; Broughton, William J

    2006-09-01

    Rhizobia are nitrogen-fixing bacteria that establish endosymbiotic associations with legumes. Nodule formation depends on various bacterial carbohydrates, including lipopolysaccharides, K-antigens, and exopolysaccharides (EPS). An acidic EPS from Rhizobium sp. strain NGR234 consists of glucosyl (Glc), galactosyl (Gal), glucuronosyl (GlcA), and 4,6-pyruvylated galactosyl (PvGal) residues with beta-1,3, beta-1,4, beta-1,6, alpha-1,3, and alpha-1,4 glycoside linkages. Here we examined the role of NGR234 genes in the synthesis of EPS. Deletions within the exoF, exoL, exoP, exoQ, and exoY genes suppressed accumulation of EPS in bacterial supernatants, a finding that was confirmed by chemical analyses. The data suggest that the repeating subunits of EPS are assembled by an ExoQ/ExoP/ExoF-dependent mechanism, which is related to the Wzy polymerization system of group 1 capsular polysaccharides in Escherichia coli. Mutation of exoK (NGROmegaexoK), which encodes a putative glycanase, resulted in the absence of low-molecular-weight forms of EPS. Analysis of the extracellular carbohydrates revealed that NGROmegaexoK is unable to accumulate exo-oligosaccharides (EOSs), which are O-acetylated nonasaccharide subunits of EPS having the formula Gal(Glc)5(GlcA)2PvGal. When used as inoculants, both the exo-deficient mutants and NGROmegaexoK were unable to form nitrogen-fixing nodules on some hosts (e.g., Albizia lebbeck and Leucaena leucocephala), but they were able to form nitrogen-fixing nodules on other hosts (e.g., Vigna unguiculata). EOSs of the parent strain were biologically active at very low levels (yield in culture supernatants, approximately 50 microg per liter). Thus, NGR234 produces symbiotically active EOSs by enzymatic degradation of EPS, using the extracellular endo-beta-1,4-glycanase encoded by exoK (glycoside hydrolase family 16). We propose that the derived EOSs (and not EPS) are bacterial components that play a crucial role in nodule formation in various legumes.

  10. Etude comparée des performances pondérales des chèvres naines de Guinée supplémentées au Leucaena leucocephala, au Gliricidia sepium ou au tourteau de coton dans l'Ouest Cameroun

    Directory of Open Access Journals (Sweden)

    Tedonkeng Pamo, E.

    2001-01-01

    Full Text Available Comparative Growth Performance of West African Dwarf Goat Supplemented with Leucaena leucocephala, Gliricidia sepium or Cotton Seed Cake in West Cameroon. A comparative growth performance study of West African Dwarf Goat supplemented with Leucaena leucocephala, Gliricidia sepium or cotton seed cake was carried out on-farm around Dschang and at the University Experimental Farm on the Western Highland region of Cameroon. Goats aged between 12 and 16 months were divided into 4 groups of 11 animals each, with one group kept as a control. The goats in the supplemented groups received 390 g of L. leucocephala, 513 g of G. sepium and 88.8 g of cotton seed cake respectively, corresponding to a supplementation 6 g of nitrogen per animal per day. Animals were weighed every 21 days during an 84 days period to evaluate their response to supplementation. The weights of animals supplemented with cotton seed cake were not significantly (P> 0.05 different from those of animals supplemented with L. leucocephala. Similarly the weights of animals supplemented with G. sepium were not different from those of the controls. The adjusted average daily weight gains per goat were 18.9 g ; 17.3 g ; 4.3 g ; and 4.0 g respectively for animals supplemented with cotton seed cake, L. Leucocephala, G. sepium and of the control group.

  11. Estudios sobre Plantas Andinas, VI

    Directory of Open Access Journals (Sweden)

    Cuatrecasas José

    1945-12-01

    Full Text Available Diplostephium subincisum Cuatr., sp. nov. / Diplostephium cinerascens Cuatr., sp, nov. / Diplostephium floribundum subsp. farallonense Cuatr. nova. / Diplostephium violaceum Cuatr., var. puracense Cuatr. nova. / Aphanactis cocuyensis Cuatr., sp. nov. / Liabum tabanense Cuatr., sp. nov. / Steiractinia aspera Cuatr., sp. nov. / Brunellia latifolia Cuatr . sp. nov. / Brunellia occidentalis Cuatr., sp.. nov. / Brunellia Trianae Cuatr., sp. nov. / Pinguicula diversifolia Cuatr., sp, nov. / Pinguicula huilensis Cuatr., sp. nov. / Hedyosmum toxicum Cuatr., sp. nov. / Hedyosmum Granizo Cuatr., sp. nov. / Hedyosmum crassifolium Cuatr. sp. nov. / Hedyosmum llanorum Cuatr., sp. nov. / Hedyosmum colombianum Cuatr., sp. nov. / Hedyosmum huilense Cuatr., sp. nov. / Hedyosmum translucidum Cuatr., sp. nov. / Gentiana cocuyana Cuatr., sp. nov.

  12. Penicillium araracuarense sp. nov., Penicillium elleniae sp. nov., Penicillium penarojense sp. nov., Penicillium vanderhammenii sp. nov. and Penicillium wotroi sp. nov., isolated from leaf litter.

    Science.gov (United States)

    Houbraken, Jos; López-Quintero, Carlos A; Frisvad, Jens C; Boekhout, Teun; Theelen, Bart; Franco-Molano, Ana Esperanza; Samson, Robert A

    2011-06-01

    Several species of the genus Penicillium were isolated during a survey of the mycobiota of leaf litter and soil in Colombian Amazon forest. Five species, Penicillium penarojense sp. nov. (type strain CBS 113178(T) = IBT 23262(T)), Penicillium wotroi sp. nov. (type strain CBS 118171(T) = IBT 23253(T)), Penicillium araracuarense sp. nov. (type strain CBS 113149(T) = IBT 23247(T)), Penicillium elleniae sp. nov. (type strain CBS 118135(T) = IBT 23229(T)) and Penicillium vanderhammenii sp. nov. (type strain CBS 126216(T) = IBT 23203(T)) are described here as novel species. Their taxonomic novelty was determined using a polyphasic approach, combining phenotypic, molecular (ITS and partial β-tubulin sequences) and extrolite data. Phylogenetic analyses showed that each novel species formed a unique clade for both loci analysed and that they were most closely related to Penicillium simplicissimum, Penicillium janthinellum, Penicillium daleae and Penicillium brasilianum. An overview of the phylogeny of this taxonomically difficult group is presented, and 33 species are accepted. Each of the five novel species had a unique extrolite profile of known and uncharacterized metabolites and various compounds, such as penicillic acid, andrastin A, pulvilloric acid, paxillin, paspaline and janthitrem, were commonly produced by these phylogenetically related species. The novel species had a high growth rate on agar media, but could be distinguished from each other by several macro- and microscopical characteristics.

  13. Neues vom Heringsparasiten Ichthyophonus sp.

    OpenAIRE

    Lang, Thomas

    1994-01-01

    Seit dem Massensterben von Heringen entlang der schwedischen Südküste im Sommer 1991, aufgrund einer Infektion mit dem parasitischen Pilz Ichthyophonus sp., wird der Gesundheitszustand der Heringsbestände in den europäischen Seegebieten intensiv überwacht. Diese Untersuchungen, an denen sich auch die Bundesforschungsanstalt für Fischerei beteiligt, werden koordiniert von der "Arbeitsgruppe über Pathologie und Krankheiten mariner Organismen" des Internationalen Rates für Meeresforschung (IC...

  14. DISTRIBUSI Solen sp DI PERAIRAN KABUPATEN BANGKALAN

    Directory of Open Access Journals (Sweden)

    Eva Ari Wahyuni

    2016-03-01

    Full Text Available DISTRIBUTION OF Solen sp IN BANGKALAN WATERSSolen sp potential needs to be developed on the island of Madura, particularly in Bangkalan. Solen sp utilization has increased which has the potential to overfishing. Therefore, this study aims to determine the density of Solen sp and their ecology in the waters Modung village, Modung District, Bangkalan. The experiment was conducted in April 2015 using the descriptive method. The materials used include Solen sp and physico-chemical parameters of the environment (temperature, salinity, pH, and substrate. The analyzes were conducted at the Laboratory of Marine Science, Department of Marine Sciences, Trunojoyo University of Madura by using the tool grabsampler, sieveshaker, and pipetting with gravimetric method. The analysis shows the range of values of temperature between 29-300C, salinity between 31-32 ppt, pH were 7.9-8.0 and the type of substrate in the form of sandy mud, as well as the density of Solen sp from 8-10 individuals/m2. All measurement results indicate normal conditions and in accordance with the sea water quality standard for marine life, which can be a suitable habitat for the growth and development of Solen sp. This condition is thought to affect the density of Solen sp.Keywords: Bangkalan, density, distribution, Solen sp, substrate.ABSTRAKPotensi Solen sp perlu dikembangkan di pulau Madura, khususnya di Kabupaten Bangkalan. Pemanfaatan Solen sp mengalami peningkatan sehingga berpotensi overfishing. Untuk itu, penelitian ini bertujuan untuk mengetahui kepadatan Solen sp dan ekologinya di perairan desa Modung, Kecamatan Modung, Kabupaten Bangkalan. Penelitian dilaksanakan pada bulan April 2015 dengan metode deskriptif. Materi dan bahan yang digunakan diantaranya Solen sp dan parameter fisika-kimia lingkungan (suhu, salinitas, pH, dan substrat. Analisa dilakukan di Laboratorium Ilmu Kelautan, Program studi/Jurusan Ilmu Kelautan Universitas Trunojoyo Madura dengan menggunakan alat

  15. Introduction to the SP theory of intelligence

    OpenAIRE

    Wolff, J Gerard

    2018-01-01

    This article provides a brief introduction to the "Theory of Intelligence" and its realisation in the "SP Computer Model". The overall goal of the SP programme of research, in accordance with long-established principles in science, has been the simplification and integration of observations and concepts across artificial intelligence, mainstream computing, mathematics, and human learning, perception, and cognition. In broad terms, the SP system is a brain-like system that takes in "New" infor...

  16. Growth of seedlings of pigeon pea (Cajanus cajan (l. millsp, wand riverhemp (Sesbania virgata (cav. pers., and lead tree (Leucaena leucocephala (lam. de wit in an arsenic-contaminated soil

    Directory of Open Access Journals (Sweden)

    Luiz Eduardo Dias

    2010-06-01

    Full Text Available Phytoremediation strategies utilize plants to decontaminate or immobilize soil pollutants. Among soil pollutants, metalloid As is considered a primary concern as a toxic element to organisms. Arsenic concentrations in the soil result from anthropogenic activities such as: the use of pesticides (herbicides and fungicides; some fertilizers; Au, Pb, Cu and Ni mining; Fe and steel production; coal combustion; and as a bi-product during natural gas extraction. This study evaluated the potential of pigeon pea (Cajanus cajan, wand riverhemp (Sesbania virgata, and lead tree (Leucaena leucocephala as phytoremediators of soils polluted by As. Soil samples were placed in plastic pots, incubated with different As doses (0; 50; 100 and 200 mg dm-3 and then sown with seeds of the three species. Thirty (pigeon pea and 90 days after sowing, the plants were evaluated for height, collar diameter and dry matter of young, intermediate and basal leaves, stems and roots. Arsenic concentration was determined in different aged leaves, stems and roots to establish the translocation index (TI between the plant root system and aerial plant components and the bioconcentration factors (BF. The evaluated species showed distinct characteristics regarding As tolerance, since the lead tree and wand riverhemp were significantly more tolerant than pigeon pea. The high As levels found in wand riverhemp roots suggest the existence of an efficient accumulation and compartmentalization mechanism in order to reduce As translocation to shoot tissues. Pigeon pea is a sensitive species and could serve as a potential bioindicator plant, whereas the other two species have potential for phytoremediation programs in As polluted areas. However, further studies are needed with longer exposure times in actual field conditions to reach definite conclusions on relative phytoremediation potentials.A fitorremediação é uma estratégia que utiliza plantas para descontaminar ou imobilizar poluentes

  17. Evaluation of antioxidant and cytotoxic properties of Cynobacteria, Limnothrix sp. and Leptolyngbya sp. from Arabian sea

    Digital Repository Service at National Institute of Oceanography (India)

    Anas, A.; Vinothkumar, S.; Gupta, S.; Jasmin, C.; Joseph, V.; Parameswaran, P.S.; Nair, S.

    The hexane fractions of the marine cyanobacteria: Leptolyngbya sp. and Limnothrix sp., collected from Arabian Sea were found to display promising antioxidant properties than their ethyl acetate fraction during radical scavenging ABTS/DPPH assays (IC...

  18. Xylanolytic enzyme systems in Arthrobacter sp MTCC 5214 and Lactobacillus sp.

    Digital Repository Service at National Institute of Oceanography (India)

    Khandeparker, R.; Jalal, T.

    The production of extracellular xylanolytic enzymes such as xylanase, alfa-L-arabinofuranosidase (alfa-l-AFase), and acetyl xylan esterase (Axe) by marine Arthrobacter sp and Lactobacillus sp was investigated using different carbon sources Induction...

  19. Pengendalian Hayati Penyakit Layu Fusarium Pisang (Fusarium Oxysporum F.sp. Cubense) dengan Trichoderma SP.

    OpenAIRE

    Sudirman, Albertus; Sumardiyono, Christanti; Widyastuti, Siti Muslimah

    2011-01-01

    The aim of this research was to study the inhibiting ability of Trichoderma sp. to control fusarium wilt of banana in greenhouse condition. The experiments consisted of the antagonism test between Trichoderma sp. and Fusarium oxysporum f.sp. cubense (Foc) in vitro using dual culture method and glass house experiment which was arranged in 3×3 Factorial Complete Randomized Design. First factor of the latter experiment was the dose of Trichoderma sp. culture (0, 25, and 50 g per polybag), second...

  20. Selective C(sp2)-C(sp) bond cleavage: the nitrogenation of alkynes to amides.

    Science.gov (United States)

    Qin, Chong; Feng, Peng; Ou, Yang; Shen, Tao; Wang, Teng; Jiao, Ning

    2013-07-22

    Breakthrough: A novel catalyzed direct highly selective C(sp2)-C(sp) bond functionalization of alkynes to amides has been developed. Nitrogenation is achieved by the highly selective C(sp2)-C(sp) bond cleavage of aryl-substituted alkynes. The oxidant-free and mild conditions and wide substrate scope make this method very practical. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. First report of Anisakis sp. in Epinephelus sp. in East Indonesia

    OpenAIRE

    Annytha Ina Rohi Detha; Diana Agustiani Wuri; Julianty Almet; Yuni Riwu; Christin Melky

    2018-01-01

    Objective: The present research was conducted to identify the prevalence of Anisakis sp. as fish-borne zoonoses in Epinephelus sp. in territorial waters of East Nusa Tenggara, Indonesia. Materials and methods: A total of 50 fish (Epinephelus sp.) were collected from Kupang Fish Market in East Nusa Tenggara. Identification of Anisakis sp. was performed based on morphological observations considering shape of ventriculus, boring tooth, and mucron using binocular microscope. Results: Prev...

  2. Pseudoxanthomonas koreensis sp. nov. and Pseudoxanthomonas daejeonensis sp. nov.

    Science.gov (United States)

    Yang, Deok-Chun; Im, Wan-Taek; Kim, Myung Kyum; Lee, Sung-Taik

    2005-03-01

    Gram-negative, non-spore-forming, rod-shaped bacteria, T7-09(T) and TR6-08(T), were isolated from soil from a ginseng field in South Korea and characterized to determine their taxonomic position. 16S rRNA gene sequence analysis showed that the two isolates shared 99.5 % sequence similarity. Strains T7-09(T) and TR6-08(T) were shown to belong to the Proteobacteria and showed the highest levels of sequence similarity to Pseudoxanthomonas broegbernensis DSM 12573(T) (98.1 %), Pseudoxanthomonas mexicana AMX 26B(T) (97.4-97.5 %), Pseudoxanthomonas japonensis 12-3(T) (96.5-96.6 %), Pseudoxanthomonas taiwanensis ATCC BAA-404(T) (95.7 %) and Xanthomonas campestris ATCC 33913(T) (96.3-96.5 %). The sequence similarity values with respect to any species with validly published names in related genera were less than 96.5 %. The detection of a quinone system with Q-8 as the predominant compound and a fatty acid profile with C(15 : 0) iso as the predominant acid supported the assignment of the novel isolates to the order 'Xanthomonadales'. The two isolates could be distinguished from the established species of the genus Pseudoxanthomonas by the presence of quantitative unsaturated fatty acid C(17 : 1) iso omega9c and by their unique biochemical profiles. The results of DNA-DNA hybridization clearly demonstrated that T7-09(T) and TR6-08(T) represent separate species. On the basis of these data, it is proposed that T7-09(T) (=KCTC 12208(T)=IAM 15116(T)) and TR6-08(T) (=KCTC 12207(T)=IAM 15115(T)) be classified as the type strains of two novel Pseudoxanthomonas species, for which the names Pseudoxanthomonas koreensis sp. nov. and Pseudoxanthomonas daejeonensis sp. nov., respectively, are proposed.

  3. Performance of Glomus clarum and Tithonia diversifolia compost in ...

    African Journals Online (AJOL)

    Screen house experiments were conducted at the Institute of Agricultural Research and Training, Ibadan, on a sandy loam soil in two years, to assess the effect of arbuscular mycorrhizal fungus (Glomus clarum) and compost (sunflower) on the growth and yield of tomato plants. The experimental design was laid out in a ...

  4. Effets des biomasses vertes de Tithonia diversifolia et des engrais ...

    African Journals Online (AJOL)

    même dans les sols peu fertiles (McKey et al.,. 2012 ; Temegne et al., 2015). Cependant, il peut aussi produire beaucoup plus lorsqu'il est cultivé avec un supplément judicieux d'engrais. La fertilisation minérale conventionnelle est incompatible avec le contexte économique du paysan camerounais du fait du coût élevé des ...

  5. Maize response to Tithonia diversifolia and rock phosphate ...

    African Journals Online (AJOL)

    SARAH

    2014-07-31

    Jul 31, 2014 ... 2Department of Agricultural Resources Management, Kenyatta University, P.O Box 43844-00100 Nairobi, Kenya. ... Original submitted in on 7th ... human population which is projected to be 8,000 .... Institute, version 9.2).

  6. CAN TITHONIA DIVERSIFOLIA (Hemsl). A. GRAY, A PAN- TROPIC ...

    African Journals Online (AJOL)

    DJFLEX

    Tithonia seedlings were able to absorb lead and cadmium in the polluted soils, ... spillage damages the soil, water and both plants and animals. ... (1969) reported that sulphur dioxide and nitrogen oxide .... organic carbon. Measured volumes ...

  7. Specialised predation by Palpimanus sp. (Araneae: Palpimanidae ...

    African Journals Online (AJOL)

    This is the first detailed report on the natural prey and the prey-capture tactics of a Palpimanus sp. from Entebbe (Uganda). Although this species fed occasionally on insects, its dominant prey in the field was other spiders, especially jumping spiders (Salticidae) and their eggs. Encounters between Palpimanus sp. and ...

  8. Kultivasi Scenedesmus SP. Pada Medium Air Limbah

    OpenAIRE

    Kawaroe, Mujizat

    2011-01-01

    Proses fotosintesis pada mikroalga membutuhkan CO2 dan cahaya matahari serta nutrien untuk pertumbuhannya. Kultivasi Scenedesmus sp. pada medium air limbah bertujuan guna mencukupi kebutuhan mikroalga akan nutrien dan mengurangi masukan dari bahan kimia yang terkandung dalam air limbah tersebut ke lingkungan. Kultivasi Scenedesmus sp. dilakukan selama tujuh hari pada medium air limbah industri tanpa penambahan nutri...

  9. Guide til gode spørgeskemaer

    DEFF Research Database (Denmark)

    Olsen, Henning

    Spørgeskemaundersøgelser bliver ofte brugt til at dokumentere forskellige forhold og begrunde politiske beslutninger. Men resultaterne kan være forbundet med stor usikkerhed. Det kræver omhu og stor sproglig formåen at udarbejde spørgeskemaer. Seniorforsker Henning Olsen har i flere år arbejdet med...... viden om, hvordan folk forstår sproglige meddelelser og genkalder sig informationer. I guiden behandles emner som fx styrende problemstillinger og spørgsmåls fokus og neutralitet, formulering af åbne eller lukkede spørgsmål og svarkategorier, tematiske spørgeforløb, aflastning af svarpersoners...

  10. Glyphosate catabolism by Pseudomonas sp

    International Nuclear Information System (INIS)

    Shinabarger, D.L.

    1986-01-01

    The pathway for the degradation of glyphosate (N-phosphonomethylglycine) by Pseudomonas sp. PG2982 has been determined using metabolic radiolabeling experiments. Radiorespirometry experiments utilizing [3- 14 C] glyphosate revealed that approximately 50-59% of the C3 carbon was oxidized to CO 2 . Fractionation of stationary phase cells labeled with [3- 14 C]glyphosate revealed that from 45-47% of the assimilated C3 carbon is distributed to proteins and that amino acids methionine and serine are highly labeled. The nucleic acid bases adenine and guanine received 90% of the C3 label that was incorporated into nucleic acids, and the only pyrimidine base labeled was thymine. Pulse labeling of PG2982 cells with [3- 14 C]glyphosate revealed that [3- 14 C]sarcosine is an intermediate in glyphosate degradation. Examination of crude extracts prepared from PG2982 cells revealed the presence of an enzyme that oxidizes sarcosine to glycine and formaldehyde. These results indicate that the first step in glyphosate degradation by PG2982 is cleavage of the carbon-phosphorus bond, resulting in the release of sarcosine and a phosphate group. The phosphate group is utilized as a source of phosphorus, and the sarcosine is degraded to glycine and formaldehyde. Phosphonate utilization by Pseudomonas sp. PG2982 was investigated. Each of the ten phosphonates tested were utilized as a sole source of phosphorus by PG2982. Representative compounds tested included alkylphosphonates, 1-amino-substituted alkylphosphonates, amino-terminal phosphonates, and an arylphosphonate. PG2982 cultures degraded phenylphosphonate to benzene and produced methane from methylphosphonate. The data indicate that PG2982 is capable of cleaving the carbon-phosphorus bond of several structurally different phosphonates

  11. Trichoderma sp. dalam Pengendalian Penyakit Layu Fusarium pada Tanaman Tomat

    OpenAIRE

    Novita, Trias

    2013-01-01

    Penelitian ini bertujuan untuk mengetahui peran Trichoderma sp dalam pengendalianpenyakit layu fusarium pada tanaman tomat. Penelitian dilaksanakan di Rumah Kaca FakultasPertanian Universitas Jambi, perlakuannya terdiri dari : t0 = tanpa Trichoderma sp; t1 = 25 gTrichoderma sp/8 kg media; t2 = 50 g Trichoderma sp/8 kg media; t3 = 75 g Trichoderma sp/8 kgmedia; dan t4 = 100 g Trichoderma sp /8 kg media. Hasil penelitian menunjukkan bahwa Trichodermasp berperan dalam mengendalikan penyakit layu...

  12. INTERAKSI ANTARA Trichoderma Harzianum, Penicillium SP. DAN Pseudomonas SP. SERTA KAPASITAS ANTAGONISMENYA TERHADAP Phytophthora CapsicilN VITRO*[Interaction Among Trichoderma Harzianum, Penicillium SP., Pseudomonas SP. and Antagonism Capacities Against Phy

    OpenAIRE

    Suharna, Nandang

    2003-01-01

    A preliminary study has been done to know antagonism capacities of three isolates of Trichoderma harzianum, two isolates of Penicillium sp.and one isolate of Pseudomonas sp.against Phytophthora capsici in vitro and interaction among those six antagonists.The highest antagonism capacity possessed by Penicillium sp. KN1, respectively followed by Penicillium sp.KN2,Pseudomonas sp. GH1 and the three T. harzianum isolates. Except for those three T. harzianum isolates, the two Penicillium sp.isolat...

  13. SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis

    Directory of Open Access Journals (Sweden)

    Mario Saare

    2015-01-01

    Full Text Available The SP100 family members comprise a set of closely related genes on chromosome 2q37.1. The widely expressed SP100 and the leukocyte-specific proteins SP110 and SP140 have been associated with transcriptional regulation and various human diseases. Here, we have characterized the SP100 family member SP140L. The genome sequence analysis showed the formation of SP140L gene through rearrangements of the two neighboring genes, SP100 and SP140, during the evolution of higher primates. The SP140L expression is interferon-inducible with high transcript levels in B cells and other peripheral blood mononuclear cells. Subcellularly, SP140L colocalizes with SP100 and SP140 in nuclear structures that are devoid of SP110, PML, or p300 proteins. Similarly to SP100 and SP140 protein, we detected serum autoantibodies to SP140L in patients with primary biliary cirrhosis using luciferase immunoprecipitation system and immunoblotting assays. In conclusion, our results show that SP140L is phylogenetically recent member of SP100 proteins and acts as an autoantigen in primary biliary cirrhosis patients.

  14. Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource

    OpenAIRE

    Srivastava, Akanksha; Tiwari, Ratnakar; Srivastava, Vikas; Singh, Tej Bali; Asthana, Ravi Kumar

    2015-01-01

    An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732) were isolated (paddy fields and ponds in the Banaras Hindu University, campus) and five strains screened for ant...

  15. Persistence of Rhizobium Inoculants originating from Leucaena ...

    African Journals Online (AJOL)

    TonukariJ

    8.0 x 104 and 9.0 x 104 rhizobia/g of soil were recovered respectively in spite of the 10-year fallow period. Biomass ... isotope subplots (6 m2) containing the 4 central trees, .... water and rain water enhanced motility of rhizobial cells from L.

  16. Ruminal and Intestinal Digestibility of Leucaena Foliage

    African Journals Online (AJOL)

    pramote

    2013-12-30

    Dec 30, 2013 ... Keywords: Intestinal digestibility, protein fodder, mobile nylon bag, a three-step technique ... A potential strategy for increasing the quality and availability of feed for small ruminants in the dry ... to measure intestinal disappearance of DM and CP using the mobile bag method described by De Boer et al.

  17. Antibacterial Actions and Potential Phototoxic Effects of Volatile oils of Foeniculum sp. (fennel, Salvia sp. (sage, Vitis sp. (grape, Lavandula sp. (lavender

    Directory of Open Access Journals (Sweden)

    Elif Ayse Erdogan Eliuz

    2016-09-01

    Full Text Available In the present study, the volatile compounds of essential oil of Foeniculum vulgare (fennel, Salvia officinalis (sage, Vitis vinifera (grape, Lavandula angustifolia (lavender were analysed by gas chromatography-mass spectrometry (GC-MS using the Nist and Willey libraries. It was determined that the main components of Foeniculum sp. were anethole (41.11%, carvacrol (9.18%. whereas main components of Salvia sp were 1.8 cineole (34.09%, caryophyllene (10.95%, camphor (9.44%, α-pinene (8.42%. Vitis sp. contained linoleic acid (36.98%, 2,4-decadienal (30.79%. Finally, volatile component of Lavandula sp. was linalool (33.57%, linalyl acetate (30.74%. Photoxic antibacterial activity of volatile oil of those plants against Escherichia coli (ATCC 25293, Klebsiella pneumoniae (10031, Salmonella thyphimurium, Bacillus subtilis (ATCC 6633, Staphylococcus aureus (ATCC 25925, Enterococcus feacalis (ATCC 29212 were examined by using disc diffusion method. We demonstrated that volatile oil effectively can be activated by a standard LED light. In vitro, significant phototoxicity was demonstrated by volatile oil of Foeniculum sp. and Vitis sp. (P < 0.05, while minor phototoxicity was induced by Lavandula sp. Therefore, volatile oil of plant can be considered as a potential photosensitizer in the photochemical therapy.

  18. TurboSP and the Topological Trigger

    CERN Document Server

    Belavin, Vladislav

    2016-01-01

    TurboSP was originally proposed as an alternative to Full stream in LHCb data flow. TurboSP is a data flow strategy which not only selects events that should be preserved, like in Full stream, but also provides selective persistence. This is achieved by saving candidates and subset of the reconstruction. During this summer project we investigated the physics viability of using TurboSP with the topological lines and found out a possibility to reduce the number of kept tracks per event by two times while keeping a ratio of fully picked up interesting decay modes on $\\sim 97 \\%$ level.

  19. Pengendalian Hayati Penyakit Layu Fusarium Pisang (Fusarium oxysporum f.sp. cubense dengan Trichoderma sp.

    Directory of Open Access Journals (Sweden)

    Albertus Sudirman

    2011-07-01

    Full Text Available The aim of this research was to study the inhibiting ability of Trichoderma sp. to control fusarium wilt of banana in greenhouse condition. The experiments consisted of the antagonism test between Trichoderma sp. and Fusarium oxysporum f.sp. cubense (Foc in vitro using dual culture method and glass house experiment which was arranged in 3×3 Factorial Complete Randomized Design. First factor of the latter experiment was the dose of Trichoderma sp. culture (0, 25, and 50 g per polybag, second factor was time of Trichoderma culture application (2 weeks before Foc inoculation, at same time with Foc inoculation and 2 weeks after Foc inoculation. Trichoderma sp. was cultured in mixed rice brand and chaff medium. The disease intensity was observed with scoring system of wilting leaves (0–4. The results showed that Trichoderma sp. was antagonistic against Foc in vitro and inhibited 86% of Foc colony development. Mechanism of antagonism between Trichoderma sp. and Foc was hyperparasitism. Trichoderma hyphae coiled around Foc hyphae. Lysis of Foc hyphae was occurred at the attached site of Trichoderma hyphae on Foc hyphae. Added banana seedling with Trichoderma sp. Culture reduced disease intensity of Fusarium wilt. Suggested dose of Trichoderma culture application in glass house was 25 g/polybag, given at the same time with Foc inoculation. Penelitian ini bertujuan untuk mengetahui kemampuan Trichoderma sp. untuk pengendalian penyakit layu fusarium pisang di rumah kaca. Penelitian meliputi pengujian daya hambat Fusarium oxysporum f.sp. cubense (Foc in vitro dan kemampuan menekan intensitas penyakit di rumah kaca. Penelitian in vitro meliputi uji antagonisme dan mekanismenya yang dilakukan secara dual culture. Uji pengaruh Trichoderma sp. terhadap penyakit layu Fusarium dilakukan di rumah kaca dengan Rancangan Acak Lengkap Faktorial. Faktor pertama adalah dosis biakan Trichoderma sp., dengan tiga aras (0, 25, 50 g/per bibit dalam polibag. Faktor kedua

  20. Detection of Salmonella sp., Vibrio sp. and total plate count bacteria on blood cockle (Anadara granosa)

    Science.gov (United States)

    Ekawati, ER; Yusmiati, S. N. H.

    2018-01-01

    Blood cockle (Anadara granosa) has high level of zinc and protein, which is beneficial for therapeutic function for malnourished particularly stunting case in children. Zinc in animal foods is more absorbable than that from vegetable food. Blood cockle (Anadara granosa) is rich in nutrient and an excellent environment for the growth of microorganisms. This research aimed to identify the contamination of Salmonella sp., Vibrio sp. and total plate count bacteria on blood cockle (Anadara granosa). This was observation research with laboratory analysis. Salmonella sp. and Vibrio sp. were detected from blood cockle. Total plate count was determine of the total amount of the bacteria. Results detected from 20 samples of blood cockle showed that all samples were negative of Salmonella sp. and 1 sample positive Vibrio sp. The result of total plate count bacteria was < 5 x 105 colony/g sample.

  1. Superhard sp2–sp3 hybrid carbon allotropes with tunable electronic properties

    Directory of Open Access Journals (Sweden)

    Meng Hu

    2016-05-01

    Full Text Available Four sp2–sp3 hybrid carbon allotropes are proposed on the basis of first principles calculations. These four carbon allotropes are energetically more favorable than graphite under suitable pressure conditions. They can be assembled from graphite through intralayer wrinkling and interlayer buckling, which is similar to the formation of diamond from graphite. For one of the sp2–sp3 hybrid carbon allotropes, mC24, the electron diffraction patterns match these of i-carbon, which is synthesized from shock-compressed graphite (H. Hirai and K. Kondo, Science, 1991, 253, 772. The allotropes exhibit tunable electronic characteristics from metallic to semiconductive with band gaps comparable to those of silicon allotropes. They are all superhard materials with Vickers hardness values comparable to that of cubic BN. The sp2–sp3 hybrid carbon allotroes are promising materials for photovoltaic electronic devices, and abrasive and grinding tools.

  2. EOP Gold Coral (Gerardia sp.) Growth Measurements

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Gold coral (Gerardia sp.) trees that were inspected years earlier on Pisces submersible dives were revisited and their change in size measured. The fishery for...

  3. Metabolites from marine fungus Aspergillus sp.

    Digital Repository Service at National Institute of Oceanography (India)

    Wahidullah, S.; Rajmanickam, R.; DeSouza, L.

    Chemical examination of a methanolic extract of the marine fungus, Aspergillus sp., isolated from marine grass environment, yielded a steroid, ergosterol peroxide (1), and a mixture of known glyceride esters (2,3) of unsaturated fatty acids...

  4. SP (4,R) symmetry in light nuclei

    International Nuclear Information System (INIS)

    Peterson, D.R.

    1979-01-01

    A classification of nuclear states according to the noncompact sympletic Lie algebras sp(2n,R), n = 1, 2, 3, is investigated. Such a classification has recently been shown to be physically meaningful. This classification scheme is the appropriate generalization fo Elliott's SU 3 model of rotational states in deformed light nuclei to include core excitations. A restricted classification according to the Lie algebra, sp(4,R), is motivated. Truncation of the model space to a single sp(4,R) irreducible representation allows the inclusion of states possessing very high excitation energy. An sp(4,R) model study is performed on S = T = 0 positive-parity rotational bands in the deformed light nuclei 16 O and 24 Mg. States are included in the model space that possess up to 10h ω in excitation energy. Results for the B(E2) transition rates compare favorable with experiment, without resort to effective charges

  5. Progress in SP-100 tribological coatings

    International Nuclear Information System (INIS)

    Ring, P.J.; Roy, P.; Schuster, G.B.; Busboom, H.J.

    1992-01-01

    The SP-100 reactor will operate at temperatures up to 1500K in high vacuum. To address the SP-100 needs, a tribology development program has been established at GE to investigate candidate coating materials. Materials were selected based on their high thermodynamic stability, high melting point, compatibility with the substrate, and coefficients of thermal expansion similar to niobium-1% zirconium-the candidate structural material for SP-100. An additional requirement was that the deposition processes should be commercially available to coat large components. This paper presents the details regarding the SP-100 Tribology Development Program including background information, specific bearing requirements, basis for coating material selection, testing methods and the initial results covering the early years of this program

  6. Silencing the SpMPK1, SpMPK2, and SpMPK3 Genes in Tomato Reduces Abscisic Acid—Mediated Drought Tolerance

    Directory of Open Access Journals (Sweden)

    Yan Liang

    2013-11-01

    Full Text Available Drought is a major threat to agriculture production worldwide. Mitogen-activated protein kinases (MAPKs play a pivotal role in sensing and converting stress signals into appropriate responses so that plants can adapt and survive. To examine the function of MAPKs in the drought tolerance of tomato plants, we silenced the SpMPK1, SpMPK2, and SpMPK3 genes in wild-type plants using the virus-induced gene silencing (VIGS method. The results indicate that silencing the individual genes or co-silencing SpMPK1, SpMPK2, and SpMPK3 reduced the drought tolerance of tomato plants by varying degrees. Co-silencing SpMPK1 and SpMPK2 impaired abscisic acid (ABA-induced and hydrogen peroxide (H2O2-induced stomatal closure and enhanced ABA-induced H2O2 production. Similar results were observed when silencing SpMPK3 alone, but not when SpMPK1 and SpMPK2 were individually silenced. These data suggest that the functions of SpMPK1 and SpMPK2 are redundant, and they overlap with that of SpMPK3 in drought stress signaling pathways. In addition, we found that SpMPK3 may regulate H2O2 levels by mediating the expression of CAT1. Hence, SpMPK1, SpMPK2, and SpMPK3 may play crucial roles in enhancing tomato plants’ drought tolerance by influencing stomatal activity and H2O2 production via the ABA-H2O2 pathway.

  7. Cellulose powder from Cladophora sp. algae.

    Science.gov (United States)

    Ek, R; Gustafsson, C; Nutt, A; Iversen, T; Nyström, C

    1998-01-01

    The surface are and crystallinity was measured on a cellulose powder made from Cladophora sp. algae. The algae cellulose powder was found to have a very high surface area (63.4 m2/g, N2 gas adsorption) and build up of cellulose with a high crystallinity (approximately 100%, solid state NMR). The high surface area was confirmed by calculations from atomic force microscope imaging of microfibrils from Cladophora sp. algae.

  8. Penggunaan Jamur Antagonis Trichoderma SP. Dan Gliocladium SP. Untuk Mengendalikan Penyakit Layu Fusarium Pada Tanaman Bawang Merah (Allium Ascalonicum L.)

    OpenAIRE

    Arie Ramadhina, Arie Ramadhina; Lisnawita, Lisnawita; Lubis, Lahmuddin

    2013-01-01

    The use of antagonism fungus of Trichoderma sp. and Gliocladium sp. for controlling wilt(Fusarium oxysporum) in red onion plants. The aim of the research was to know the effectiviness ofantagonism fungus of Trichoderma sp. and Gliocladium sp. in controlling wilt in red onion plants.The research used non-factorial RAK (random group design) with eight treatments: control, 10grams of F. oxysporum, 12 grams of Trichoderma sp., 18 grams of Trichoderma sp., 24 grams ofTrichoderma sp., and 12 grams ...

  9. Interaction of uranium with Pleurotus sp

    International Nuclear Information System (INIS)

    Ohnuki, Toshihiko; Sakamoto, Fuminori; Kozaki, Naofumi; Ozaki, Takuro; Samadfam, Mohammad

    2002-01-01

    Uptake of uranium by higher fungi, such as mushroom is little elucidated. We have studied the interaction of uranium with Pleurotus sp. (a mushroom) in pure culture over a wide range of U concentration (50-3000 mg/L). The Pleurotus sp. was cultured in two different media. One was rice bran medium, and the other was agar (yeast extract, peptone and dextrose) medium. The uptake of uranium in Pleurotus sp. was examined by alpha ray autoradiography (A,A), X-ray fluorescence spectroscopy (XRF) and scanning microcopy (SEM) equipped with EDS. In the agar medium, the higher uranium concentration gave lower growth of mycelia, and no fruiting body was observed. In the rice bran medium, the fruiting body was grown at U concentrations up to 1000 mg/L. The AA and XRF analysis showed that uranium taken up in the fruiting body was below the detection limit. The SEM-EDS analysis indicated that U was distributed in the limited region and was not transported to the mycelia far from U containing medium. It is concluded that uranium affects the growth of Pleurotus sp., and little uranium is taken up by Pleurotus sp. during the growth of both mycelia and fruiting body. (author)

  10. Taxonomy Icon Data: Synechocystis sp.PCC 6803 [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available Synechocystis sp.PCC 6803 Synechocystis sp.PCC 6803 Synechocystis_sp_PCC_6803_L.png Synecho...cystis_sp_PCC_6803_NL.png Synechocystis_sp_PCC_6803_S.png Synechocystis_sp_PCC_6803_NS.png http://bi...osciencedbc.jp/taxonomy_icon/icon.cgi?i=Synechocystis+sp%2ePCC+6803&t=L http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Synecho...cystis+sp%2ePCC+6803&t=NL http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Synecho...cystis+sp%2ePCC+6803&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Synechocystis

  11. Five novel Wickerhamomyces- and Metschnikowia-related yeast species, Wickerhamomyces chaumierensis sp. nov., Candida pseudoflosculorum sp. nov., Candida danieliae sp. nov., Candida robnettiae sp. nov. and Candida eppingiae sp. nov., isolated from plants

    NARCIS (Netherlands)

    Groenewald, Marizeth; Robert, Vincent; Smith, Maudy Th

    On the basis of nucleotide divergences in the D1/D2 domain of the 26S rRNA gene and the internal transcribed spacers (ITS) domain of the rRNA gene, five novel yeast species, Wickerhamomyces chaumierensis sp. nov. (CBS 8565(T)  = JCM 17246(T)), Candida pseudoflosculorum sp. nov. (CBS 8584(T)  = JCM

  12. SP2Bench: A SPARQL Performance Benchmark

    Science.gov (United States)

    Schmidt, Michael; Hornung, Thomas; Meier, Michael; Pinkel, Christoph; Lausen, Georg

    A meaningful analysis and comparison of both existing storage schemes for RDF data and evaluation approaches for SPARQL queries necessitates a comprehensive and universal benchmark platform. We present SP2Bench, a publicly available, language-specific performance benchmark for the SPARQL query language. SP2Bench is settled in the DBLP scenario and comprises a data generator for creating arbitrarily large DBLP-like documents and a set of carefully designed benchmark queries. The generated documents mirror vital key characteristics and social-world distributions encountered in the original DBLP data set, while the queries implement meaningful requests on top of this data, covering a variety of SPARQL operator constellations and RDF access patterns. In this chapter, we discuss requirements and desiderata for SPARQL benchmarks and present the SP2Bench framework, including its data generator, benchmark queries and performance metrics.

  13. The gamma ray spectrometer GA.SP

    Energy Technology Data Exchange (ETDEWEB)

    Bazzacco, D [Instituto Nazionale di Fisica Nucleare, Sezione di Padova, Padova (Italy)

    1992-08-01

    GA.SP is a general purpose 4{pi} detector array for advanced {gamma}-spectroscopy and, in the same time, a suitable system for reaction mechanism studies. The detector is sited at the LNL Tandem+Linac accelerator and has been built as a joint project of INFN Padova, LNL, Milano and Firenze. The array consists of 40 Compton suppressed HPGe detectors and of a 4{pi} calorimeter composed of 80 BGO crystals. The detector houses a reaction chamber of 34 cm diameter where a charged particles multiplicity filter composed of 40 Si detectors is going to be installed. Evaporation residues produced in the centre of GA.SP can be injected into the recoil mass spectrometer (RMS, named CAMEL) in use at LNL, without the need to remove any of the gamma detectors. The coupled operation of GA.SP, RMS and Si ball will give a unique instrument for identification and study of weak reaction channels. (author). 6 figs.

  14. The gamma ray spectrometer GA.SP

    International Nuclear Information System (INIS)

    Bazzacco, D.

    1992-01-01

    GA.SP is a general purpose 4π detector array for advanced γ-spectroscopy and, in the same time, a suitable system for reaction mechanism studies. The detector is sited at the LNL Tandem+Linac accelerator and has been built as a joint project of INFN Padova, LNL, Milano and Firenze. The array consists of 40 Compton suppressed HPGe detectors and of a 4π calorimeter composed of 80 BGO crystals. The detector houses a reaction chamber of 34 cm diameter where a charged particles multiplicity filter composed of 40 Si detectors is going to be installed. Evaporation residues produced in the centre of GA.SP can be injected into the recoil mass spectrometer (RMS, named CAMEL) in use at LNL, without the need to remove any of the gamma detectors. The coupled operation of GA.SP, RMS and Si ball will give a unique instrument for identification and study of weak reaction channels. (author). 6 figs

  15. SP-100/Brayton power system concepts

    International Nuclear Information System (INIS)

    Owen, D.F.

    1989-01-01

    Use of closed Brayton cycle (CBC) power conversion technology has been investigated for use with SP-100 reactors for space power systems. The CBC power conversion technology is being developed by Rockwell International under the Dynamic Isotype Power System (DIPS) and Space Station Freedom solar dynamic power system programs to provide highly efficient power conversion with radioisotype and solar collector heat sources. Characteristics including mass, radiator area, thermal power, and operating temperatures for systems utilizing SP-100 reactor and CBC power conversion technology were determined for systems in the 10-to 100-kWe power range. Possible SP-100 reactor/CBC power system configurations are presented. Advantages of CBC power conversion technology with regard to reactor thermal power, operating temperature, and development status are discussed

  16. Modifications of Sp(2) covariant superfield quantization

    Energy Technology Data Exchange (ETDEWEB)

    Gitman, D.M.; Moshin, P.Yu

    2003-12-04

    We propose a modification of the Sp(2) covariant superfield quantization to realize a superalgebra of generating operators isomorphic to the massless limit of the corresponding superalgebra of the osp(1,2) covariant formalism. The modified scheme ensures the compatibility of the superalgebra of generating operators with extended BRST symmetry without imposing restrictions eliminating superfield components from the quantum action. The formalism coincides with the Sp(2) covariant superfield scheme and with the massless limit of the osp(1,2) covariant quantization in particular cases of gauge-fixing and solutions of the quantum master equations.

  17. SP-100 converter multicouple thermoelectric cell

    International Nuclear Information System (INIS)

    Kull, R.A.; Terrill, W.R.

    1990-01-01

    The General Electric Company is under contract to DOE to design, fabricate, and test an SP-100 Ground Engineering System. This paper provides a description of the SP-100 space reactor power system configuration, and a more detailed description of the power conversion subsystem (PCSS) and the key building block of the power converter, the thermoelectric cell. The functions of the various elements of the PCSS and the cells are also presented. These cells convert the thermal energy from the reactor into electrical power at the desired voltage while being conductively coupled to the hot and cold side heat exchangers to maximize the power output and system specific power

  18. Fatty acid composition of Spirulina sp., Chlorella sp. and Chaetoceros sp. microalgae and introduction as potential new sources to extinct omega 3 and omega 6

    Directory of Open Access Journals (Sweden)

    Homan Gorjzdadeh

    2016-05-01

    Full Text Available Background: This study was carried out to determine the oil fatty acids from two special species of microalgae; Spirulina sp.,Chlorella sp. and also Chaetoceros sp. collected from Bahmanshir River. Materials and Methods: Sampling of microalgae Chaetoceros sp. from Bahmanshir River was under taken using bottle samplers during spring season of 2013. Microalgae Spirulina sp. and Chlorella sp. were supplied from Shrimp Research Institute of Iran in Bushehr Province. Samples then were cultured under controlled laboratory conditions and mass culture for 100 liters was undertaken. Isolation of microalgae species from water of cultured media was carried out using filtration and centrifugation methods. The fatty acid compositions were determined by Gas – FID chromatography. Results: Results showed that regarding Saturated Fatty Acids (SFA obtained from purified culture of Chaetoceros sp., Spirulina sp. and Chlorella sp. the maximum amount of total fatty acids were belonged to palmitic acids (C16:0 with 15.21%, 30.1% and 25.17% of total fatty acids  respectively. Analysis of Mono Unsaturated Fatty Acids (MUFA showed that in the Oleic acid was maximum amount of 34% in Spirulina sp. In addition the amount of MUFA in Chlorella sp. was 16.37% of total fatty acids. On the other hand the amount of palmeotic acid in purified culture of Chaetoceros sp. was 30.33% from total content of fatty acids. Analysis of Poly Unsaturated Fatty Acids (PUFA, Linoleic acid (C18:2 (Omega 6, revealed maximum percentage in Spirulina sp. with 18.8%. Results of Alpha linoleic acid (C18:3 (Omega3 analysis showed maximum amount of 9.66% in Chlorella sp. compared to other microalgae with lower omega 3 contents. Spirulina sp. contained maximum amount of Linoleic acid (C18:2 with 18.8% of total fatty acids. Therefore, Spirulina sp. can be considered as a rich source of omega 6 for the purpose of fatty acid extractions. The presence of PUFA in Chlorella sp. and Spirulina sp. was

  19. Halobacterium sp. SP1(1) as a starter culture for accelerating fish sauce fermentation.

    Science.gov (United States)

    Akolkar, A V; Durai, D; Desai, A J

    2010-07-01

    Application of Halobacterium sp. SP1(1) for the acceleration of fish sauce fermentation. Traditional fish sauce fermentation was mimicked using Halobacterium sp. SP1(1) as starter culture. Protease activity, peptide release and α-amino content (parameters used to monitor the progress of the fermentation) were high at day 10 in tests and day 20 in un-inoculated controls. The total protein and nitrogen contents were also high in tests compared with controls. The amino acid profile observed at the end of fermentation in experimental samples, when compared with the commercial sauce preparation, was found to be better with respect to flavour and aroma contributing amino acids as well as essential amino acid lysine. Microflora analysis of the final fish sauce revealed the absence of any nonhalophilic or halotolerant micro-organisms. The protease-producing halophilic isolates obtained from the fish sauce of eviscerated and uneviscerated controls were identified as Halobacterium sp. F1 and F2, respectively, by 16S rDNA sequence analysis. Exogenous augmentation of Halobacterium sp. SP1(1) accelerated the fish sauce fermentation process with an additive effect on the existing natural microflora present in the fish during fermentation. Halobacterium sp SP1(1), therefore, can be used as an important starter culture for accelerating the fish fermentation process, which is attributed to its extracellular protease. The present study is the first report on use of Halobacterium species as a starter culture for accelerating fish sauce fermentation. Use of halobacterial starter cultures may revolutionize the process in fish sauce industries by reducing the fermentation time and making the process more economical with improved nutritive value of product. Journal compilation © 2009 The Society for Applied Microbiology. No claim to Indian Government works.

  20. 76 FR 56876 - Proposed Collection; Comment Request for Forms 9779, 9779(SP), 9783, 9783(SP), 9787, 9787(SP...

    Science.gov (United States)

    2011-09-14

    ... and 9789(SP), Electronic Federal Tax Payment System (EFTPS). DATES: Written comments should be received on or before November 14, 2011 to be assured of consideration. ADDRESSES: Direct all [email protected] . SUPPLEMENTARY INFORMATION: Title: Electronic Federal Tax Payment System (EFTPS). OMB...

  1. SP-A binding sites on bovine alveolar macrophages.

    Science.gov (United States)

    Plaga, S; Plattner, H; Schlepper-Schaefer, J

    1998-11-25

    Surfactant protein A (SP-A) binding to bovine alveolar macrophages was examined in order to characterize SP-A binding proteins on the cell surface and to isolate putative receptors from these cells that could be obtained in large amounts. Human SP-A, unlabeled or labeled with gold particles, was bound to freshly isolated macrophages and analyzed with ELISA or the transmission electron microscope. Binding of SP-A was inhibited by Ca2+ chelation, by an excess of unlabeled SP-A, or by the presence of 20 mg/ml mannan. We conclude that bovine alveolar macrophages expose binding sites for SP-A that are specific and that depend on Ca2+ and on mannose residues. For isolation of SP-A receptors with homologous SP-A as ligand we isolated SP-A from bovine lung lavage. SDS-PAGE analysis of the purified SP-A showed a protein of 32-36 kDa. Functional integrity of the protein was demonstrated. Bovine SP-A bound to Dynabeads was used to isolate SP-A binding proteins. From the fractionated and blotted proteins of the receptor preparation two proteins bound SP-A in a Ca2+-dependent manner, a 40-kDa protein showing mannose dependency and a 210-kDa protein, showing no mannose sensitivity. Copyright 1998 Academic Press.

  2. Ovos de Toxocara sp. e larvas de Ancylostoma sp. em praça pública de Lavras, MG Toxocara sp. eggs and Ancylostoma sp. larva in public parks, Brazil

    Directory of Open Access Journals (Sweden)

    Antônio Marcos Guimarães

    2005-04-01

    Full Text Available Larva migrans visceral e cutânea são zoonoses parasitárias causadas pela infecção da larva de Toxocara sp. e Ancylostoma sp., respectivamente. O objetivo do estudo foi verificar a contaminação por ovos de Toxocara sp. e ovos e larvas de Ancylostoma sp. em amostras de solos coletadas de praças públicas e de áreas de recreação infantil de Lavras, Estado de Minas Gerais, por meio da técnica de centrífugo-flutuação e do método de Baermann. A ocorrência de ovos de Toxocara sp. e, ovos e larvas de Ancylostoma sp. foi observada em 69,6% (16/23 das amostras de solo coletadas de praças públicas. A contaminação somente por ovos de Ancylostoma sp. em amostras de solo coletadas em escolas/creches foi de 22,2% (4/18. A percentagem de amostras de areia coletadas de escolas/creches contaminadas somente com larvas de Ancylostoma sp. foi de 11,1% (2/18. Praças públicas são as áreas com maior risco potencial de infecção por Toxocara sp. e Ancylostoma sp. Exame coproparasitológico realizado em 174 amostras de fezes de cães observou 58% e 23%, respectivamente, com ovos de Ancylostoma sp. e Toxocara sp.Visceral and cutaneous larva migrans are parasitic zoonoses caused by the infection of larval nematodes Toxocara sp. and Ancylostoma sp. respectively. The objective of this study was to investigate the contamination by Toxocara sp. eggs and Ancylostoma sp. eggs and larva of soil samples collected from public parks and children's playground areas in state of Minas Gerais, Brazil, using both Baermann's method and centrifugal flotation technique. Toxocara sp. and Ancylostoma sp. eggs were observed in soil samples collected from public squares in 17.4% (4/23 and 69.6 (16/23 respectively. In schools and child day care settings the contamination by Ancylostoma sp. larva in sand samples was 11.1% (2/18. Public parks are settings of more potential risk of Toxocara sp. eggs and Ancylostoma sp. infection. Stool parasitology testing of 174 stool

  3. Gilliamella intestini sp. nov., Gilliamella bombicola sp. nov., Gilliamella bombi sp. nov. and Gilliamella mensalis sp. nov.: Four novel Gilliamella species isolated from the bumblebee gut.

    Science.gov (United States)

    Praet, Jessy; Cnockaert, Margo; Meeus, Ivan; Smagghe, Guy; Vandamme, Peter

    2017-06-01

    Spectra of five isolates (LMG 28358 T , LMG 29879 T , LMG 29880 T , LMG 28359 T and R-53705) obtained from gut samples of wild bumblebees of Bombus pascuorum, Bombus lapidarius and Bombus terrestris were grouped into four MALDI-TOF MS clusters. RAPD analysis revealed an identical DNA fingerprint for LMG 28359 T and R-53705 which also grouped in the same MALDI-TOF MS cluster, while different DNA fingerprints were obtained for the other isolates. Comparative 16S rRNA gene sequence analysis of the four different strains identified Gilliamella apicola NCIMB 14804 T as nearest neighbour species. Average nucleotide identity values of draft genome sequences of the four isolates and of G. apicola NCIMB 14804 T were below the 96% threshold value for species delineation and all four strains and G. apicola NCIMB 14804 T were phenotypically distinct. Together, the draft genome sequences and phylogenetic and phenotypic data indicate that the four strains represent four novel Gilliamella species for which we propose the names Gilliamella intestini sp. nov., with LMG 28358 T as the type strain, Gilliamella bombicola sp. nov., with LMG 28359 T as the type strain, Gilliamella bombi sp. nov., with LMG 29879 T as the type strain and Gilliamella mensalis sp. nov., with LMG 29880 T as the type strain. Copyright © 2017 Elsevier GmbH. All rights reserved.

  4. Biosorption of uranium by Azolla, SP, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Ludmila C.; Alves, Eliakim G.; Marumo, Julio T., E-mail: lcvieira@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Ferreira, Rafael V. de P., E-mail: rafael@itatijuca.com [Itatijuca Biotech, Sao Paulo, SP (Brazil); Canevesi, Rafael L.S.; Silva, Edson A., E-mail: edson.silva2@unioeste.br [Universidade Estadual do Oeste Parana (UNIOESTE), Toledo, PR (Brazil)

    2015-07-01

    Radioactive liquid waste needs special attention and requires suitable treatment before deposition. Among the potential technologies under development for the treatment of liquid radioactive wastes the biosorption has been highlighted by being an efficient and low cost technique. Biosorption process involves the exchange of ions contained in the biomass matrix by others present in solution. There are many biomasses that could be applied in treatment of radioactive wastes, for example, agricultural residues and macrophyte. The aim of this study is evaluate the ability of the Azolla sp., a floating aquatic plant, to absorb uranium in solution. Azolla sp. is a macrophyte that has been used to treat effluents containing heavy metals. The biosorption capacity of uranium by Azolla sp. was experimentally determined and modeled by isotherms. Experiments were performed to determine metal uptake, and then the solutions were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES). The isotherms applied to model the data was Langmuir, Freundlich, Sips Toth, Redlich Peternson, Two-Site-Langmuir, Radke Prausnitz to develop a technique for the treatment of radioactive liquid waste generated at the Nuclear and Energy Research Institute (IPEN-CNEN/SP), Brazil. (author)

  5. Biosorption of uranium by Azolla, SP, Brazil

    International Nuclear Information System (INIS)

    Vieira, Ludmila C.; Alves, Eliakim G.; Marumo, Julio T.; Ferreira, Rafael V. de P.; Canevesi, Rafael L.S.; Silva, Edson A.

    2015-01-01

    Radioactive liquid waste needs special attention and requires suitable treatment before deposition. Among the potential technologies under development for the treatment of liquid radioactive wastes the biosorption has been highlighted by being an efficient and low cost technique. Biosorption process involves the exchange of ions contained in the biomass matrix by others present in solution. There are many biomasses that could be applied in treatment of radioactive wastes, for example, agricultural residues and macrophyte. The aim of this study is evaluate the ability of the Azolla sp., a floating aquatic plant, to absorb uranium in solution. Azolla sp. is a macrophyte that has been used to treat effluents containing heavy metals. The biosorption capacity of uranium by Azolla sp. was experimentally determined and modeled by isotherms. Experiments were performed to determine metal uptake, and then the solutions were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES). The isotherms applied to model the data was Langmuir, Freundlich, Sips Toth, Redlich Peternson, Two-Site-Langmuir, Radke Prausnitz to develop a technique for the treatment of radioactive liquid waste generated at the Nuclear and Energy Research Institute (IPEN-CNEN/SP), Brazil. (author)

  6. Carbon sp chains in graphene nanoholes

    DEFF Research Database (Denmark)

    Castelli, Ivano Eligio; Ferri, Nicola; Onida, Giovanni

    2012-01-01

    Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. ...

  7. Rhodotorula bloemfonteinensis sp. nov., Rhodotorula eucalyptica sp. nov., Rhodotorula orientis sp. nov. and Rhodotorula pini sp. nov., yeasts isolated from monoterpene-rich environments.

    Science.gov (United States)

    Pohl, Carolina H; Smit, Martha S; Albertyn, Jacobus

    2011-09-01

    Recent rDNA sequencing of 25 isolates from a previous study, during which limonene-utilizing yeasts were isolated from monoterpene-rich environments by using 1,4-disubstituted cyclohexanes as sole carbon sources, led to the identification of four hitherto unknown Rhodotorula species. Analyses of the 26S rDNA D1/D2 region as well as the internal transcribed spacer (ITS) domain indicated that two isolates (CBS 8499(T) and CBS 10736) were identical and were closely related to Rhodotorula cycloclastica, a previously described limonene-utilizing yeast. These novel isolates differed from known yeast species and could be distinguished from R. cycloclastica by standard physiological tests. The other three isolates represent three novel Rhodotorula species, closely related to Sporobolomyces magnisporus. These three species could also be distinguished from other Rhodotorula species by standard physiological tests. Based on these results, we suggest that the new isolates represent novel species, for which the names Rhodotorula eucalyptica sp. nov. (type strain CBS 8499(T)  = NRRL Y-48408(T)), Rhodotorula pini sp. nov. (type strain CBS 10735(T)  = NRRL Y-48410(T)), Rhodotorula bloemfonteinensis sp. nov. (type strain CBS 8598(T)  = NRRL Y-48407(T)) and Rhodotorula orientis sp. nov. (type strain CBS 8594(T)  = NRRL Y-48719(T)) are proposed. R. eucalyptica and R. pini can also utilize limonene.

  8. Test af spørgeskema

    DEFF Research Database (Denmark)

    Hermansen, Jonathan

    2017-01-01

    Denne bog gennemgår alle surveyarbejdets faser og giver en praktisk indføring i •design af undersøgelsen og udvælgelse af stikprøver, •formulering af spørgeskemaer samt indsamling og kodning af data, •metoder til at analysere resultaterne....

  9. Lipid contents of the sponge Haliclona sp.

    Digital Repository Service at National Institute of Oceanography (India)

    Parameswaran, P.S.; Das, B.; Kamat, S.Y.

    Several fatty acids, sterols, batyl alcohol and its analogs and an N-acylated sphingosine (ceramide) have been isolated from the lipid fraction of the extract of the sponge Haliclona sp. The major sterol is found to be cholesterol (54%), followed...

  10. Optisk scanning af spørgeskemaer

    DEFF Research Database (Denmark)

    Paulsen, Aksel

    / 1000 felter= 0.370 (95% CI: 0.160-0.729), (p= 0.020)). Der var ingen statistisk forskel mellem optisk scanning (fejl/ 1000 felter= 0.046 (95% CI: 0.001-0.258)) og manuel dobbelt indtastning (p=1.000). Konklusioner: Optisk scanning er et godt alternativ til manuel dobbelt indtastning for spørgeskema med......Formål og baggrund: Patient rapporterede outcomes i form af spørgeskemaer bruges i øgende grad i sundhedssektoren både i klinisk praksis og forskning. Ofte bruges spørgeskemaer i papirformat. Manuel dobbelt indtastning er defineret som guld standard for overføring af data til et elektronisk format......, men processen er tidkrævende. Optisk scanning af spørgeskemaer med automatisk registrering af svar kan være et alternativ, men videre validering af metoden er nødvendig. Design: 200 patienter blev tilfældigt udvalgt fra en kohorte på 5777 patienter der tidligere havde svaret på to forskellige...

  11. Aerococcus sp. with an antimycobacterial effect

    African Journals Online (AJOL)

    Yomi

    2011-12-21

    Dec 21, 2011 ... In this study, we reported data on a strain that was isolated from different areas of Fez. (Morocco), which ... sp. The antibacterial activity of the genus Aerococcus have .... able to amplify the 16S rRNA gene from eubacteria.

  12. Botrallin from the endophytic fungus Hyalodendriella sp ...

    African Journals Online (AJOL)

    use

    2011-12-12

    Dec 12, 2011 ... Bioassay-guided fractionation of the crude methanol extract of the mycelia from the endophytic fungus. Hyalodendriella sp. Ponipodef12, associated with the hybrid 'Neva' of Populus deltoides Marsh × P. nigra L., led to the isolation of one compound coded as P12-1 which was identified as botrallin (1,7-.

  13. The astaxanthin dideoxyglycoside biosynthesis pathway in Sphingomonas sp. PB304

    DEFF Research Database (Denmark)

    Kim, Se Hyeuk; Kim, Jin Ho; Lee, Bun Yeol

    2014-01-01

    A major carotenoid in Sphingomonas sp. PB304, originally isolated from a river in Daejon City, South Korea, was identified as astaxanthin dideoxyglycoside. Gene clusters encoding the astaxanthin dideoxyglycoside biosynthetic enzymes were identified by screening Sphingomonas sp. PB304 fosmid...

  14. SP-100 initial startup and restart control strategy

    Science.gov (United States)

    Halfen, Frank J.; Wong, Kwok K.; Switick, Dennis M.; Shukla, Jaikaran N.

    Startup control strategies for SP-100 are described. Revised control and operating strategies are discussed which have been developed and tested using the SP-100 dynamic simulation model Aries-GFS (Generic Flight System).

  15. Methylobacterium suomiense sp. nov. and Methylobacterium lusitanum sp. nov., aerobic, pink-pigmented, facultatively methylotrophic bacteria.

    Science.gov (United States)

    Doronina, Nina V; Trotsenko, Yuri A; Kuznetsov, Boris B; Tourova, Tatjana P; Salkinoja-Salonen, Mirja S

    2002-05-01

    Two aerobic, pink-pigmented, facultatively methylotrophic bacteria, strains F20T and RXM(T), are described taxonomically. On the basis of their phenotypic and genotypic properties, the isolates are proposed as novel species of the genus Methylobacterium, Methylobacterium suomiense sp. nov. (type strain F20T = VKM B-2238T = NCIMB 13778T) and Methylobacterium lusitanum sp. nov. (type strain RXMT = VKM B-2239T = NCIMB 13779T).

  16. Analisis Fosfor pada Cacing Tanah (Megascolex sp. dan Fridericia sp.) Secara Spektrofotometri Sinar Tampak

    OpenAIRE

    Safira, Cut Shafa

    2015-01-01

    Earthworm is natural resource which can be used for medication due to its highly amount of minerals. One of these minerals is phosphorus. The aim of this research are to identify, determine and know the difference content of phosphorus in Megascolex sp. and Fridericia sp. Qualitative analysis shows positive results with addition of ammonium molybdate 4% and BaCl2 5%. Quantitative analysis was done using visible spectrophotometer with ascorbic acid method, measuring blu-colored molybdenum ...

  17. Nanocrystalline sp{sup 2} and sp{sup 3} carbons: CVD synthesis and applications

    Energy Technology Data Exchange (ETDEWEB)

    Terranova, M. L. [Università degli Studi di Roma “Tor Vergata,” via Della Ricerca Scientifica, Dipartimento di Scienze e Tecnologie Chimiche—MinimaLab (Italy); Rossi, M. [Università degli Studi di Roma “Sapienza,” via A. Scarpa, Dipartimento di Scienze di Base e Applicate per l’Ingegneria and Centro di Ricerca per le Nanotecnologie Applicate all’Ingegneria (CNIS) (Italy); Tamburri, E., E-mail: emanuela.tamburri@uniroma2.it [Università degli Studi di Roma “Tor Vergata,” via Della Ricerca Scientifica, Dipartimento di Scienze e Tecnologie Chimiche—MinimaLab (Italy)

    2016-11-15

    The design and production of innovative materials based on nanocrystalline sp{sup 2}- and sp{sup 3}-coordinated carbons is presently a focus of the scientific community. We present a review of the nanostructures obtained in our labs using a series of synthetic routes, which make use of chemical vapor deposition (CVD) techniques for the selective production of non-planar graphitic nanostructures, nanocrystalline diamonds, and hybrid two-phase nanostructures.

  18. Differences in nutrient uptake capacity of the benthic filamentous algae Cladophora sp., Klebsormidium sp. and Pseudanabaena sp. under varying N/P conditions.

    Science.gov (United States)

    Liu, Junzhuo; Vyverman, Wim

    2015-03-01

    The N/P ratio of wastewater can vary greatly and directly affect algal growth and nutrient removal process. Three benthic filamentous algae species Cladophora sp., Klebsormidium sp. and Pseudanabaena sp. were isolated from a periphyton bioreactor and cultured under laboratory conditions on varying N/P ratios to determine their ability to remove nitrate and phosphorus. The N/P ratio significantly influenced the algal growth and phosphorus uptake process. Appropriate N/P ratios for nitrogen and phosphorus removal were 5-15, 7-10 and 7-20 for Cladophora sp., Klebsormidium sp. and Pseudanabaena sp., respectively. Within these respective ranges, Cladophora sp. had the highest biomass production, while Pseudanabaena sp. had the highest nitrogen and phosphorus contents. This study indicated that Cladophora sp. had a high capacity of removing phosphorus from wastewaters of low N/P ratio, and Pseudanabaena sp. was highly suitable for removing nitrogen from wastewaters with high N/P ratio. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Anomalous self potential (sp) log signatures observed in a water ...

    African Journals Online (AJOL)

    Geophysical logging was done after drilling had been completed in a water well at Okwudor, South Eastern Nigeria. Three electric logs were run viz: Self Potential (SP), Resistivity N16″ and N64″ logs. An anomaly was observed in the SP log. The SP results from this well show some deviation from the standard norm.

  20. Biosorption of chromium by mangrove-derived Aplanochytrium sp ...

    African Journals Online (AJOL)

    The microbial dried biomass of Thraustochytrids is used as bioadsorbent for the removal of the chromium in aqueous solution. In this investigation, three species of Thraustochydrids namely Aplanochytrium sp., Thraustochytrium sp. and Schizochytrium sp. were tested for the efficiency of chromium accumulation by culturing ...

  1. Babesia peircei sp. nov. from the jackass penguin

    African Journals Online (AJOL)

    1992-01-09

    Jan 9, 1992 ... An avian piroplasm, Babesia peircei sp. nov. is described from the jackass penguin Spheniscus demersus. Morphological differences between Babesia peircei sp. nov. and the other valid Babesia spp. are discussed together with the possible vectors. 'n Voal-piroplasma, Babesia peircei sp. nov. afkomstig ...

  2. Environmental conditions affecting exopolysaccharide production by Pseudomonas aeruginosa, Micrococcus sp., and Ochrobactrum sp.

    Science.gov (United States)

    Kiliç, Nur Koçberber; Dönmez, Gönül

    2008-06-15

    Three different chromium-resistant microorganisms (Pseudomonas aeruginosa, Micrococcus sp., and Ochrobactrum sp.) were tested with regard to their EPS production at different pH levels, temperatures, Cr(VI) concentrations, and incubation periods. The optimum pH level was 7 for P. aeruginosa and Micrococcus sp., while it was 8 for Ochrobactrum sp. according to the highest EPS amount at 100 mg/L Cr(VI) concentration. The highest production of EPSs by the three bacteria was obtained under different environmental conditions. P. aeruginosa produced the highest EPS (863.3 mg/L) after incubation for 96 h on media with 50 mg/L Cr(VI) at 20 degrees C, Micrococcus sp. gave the highest yield (444.6 mg/L) after incubation for 72 h on media with 100 mg/L Cr(VI) at the same temperature, and Ochrobactrum sp. had the highest production (430.5 mg/L) on media with 150 mg/L Cr(VI) at 30 degrees C at the end of 48 h of incubation.

  3. Remote sensing data of SP Mountain and SP Lava flow in North-Central Arizona

    Science.gov (United States)

    Schaber, G.G.; Elachi, C.; Farr, T.G.

    1980-01-01

    Multifrequency airborne radar image data of SP Mountain [Official name of feature (U.S. Geological Survey, 1970)] and SP flow (and vicinity) in north-central Arizona were obtained in diverse viewing directions and direct and cross-polarization, then compared with surface and aerial photography, LANDSAT multispectral scanner data, airborne thermal infrared imagery, surface geology, and surface roughness statistics. The extremely blocky, basaltic andesite of SP flow is significantly brighter on direct-polarization K-band (0.9-cm wavelength) images than on cross-polarized images taken simultaneously. Conversely, for the longer wavelength (25 cm) L-band radar images, the cross-polarization image returns from SP flow are brighter than the direct-polarized image. This effect is explained by multiple scattering and the strong wavelength dependence of polarization effects caused by the rectilinear basaltic andesite scatters. Two distinct types of surface relief on SP flow, one extremely blocky, the other subdued, are found to be clearly discriminated on the visible and thermal wavelength images but are separable only on the longer wavelength L-band radar image data. The inability of the K- and X- (3-cm wavelength) band radars to portray the differences in roughness between the two SP flow surface units is attributed to the radar frequency dependence of the surface-relief scale, which, described as the Rayleigh criterion, represents the transition between quasispecular and primarily diffuse backscatter. ?? 1980.

  4. Two new species of suctorians, Acineta satyanandani sp. nov. and Paracineta karunakarani sp. nov. epizoic on ostracods

    Digital Repository Service at National Institute of Oceanography (India)

    Santhakumari, V.

    Two, species of protozoic suctorians, Acineta satyanandani sp. nov. and Paracineta karunakarani sp. nov., are described. These were found attached on the body of the marine ostracod, Cypridina dentata (Muller), collected from the shelf and slope...

  5. Microbiologically influenced corrosion of galvanized steel by Desulfovibrio sp. and Desulfosporosinus sp. in the presence of Ag–Cu ions

    Energy Technology Data Exchange (ETDEWEB)

    Ilhan-Sungur, Esra, E-mail: esungur@istanbul.edu.tr [Istanbul University, Faculty of Science, Department of Biology, 34134 Vezneciler, Istanbul (Turkey); Unsal-Istek, Tuba [Istanbul University, Faculty of Science, Department of Biology, 34134 Vezneciler, Istanbul (Turkey); Cansever, Nurhan [Yıldız Technical University, Faculty of Chemistry-Metallurgy, Metallurgical and Materials Engineering Department, 34210 Esenler, Istanbul (Turkey)

    2015-07-15

    The effects of Ag–Cu ions on the microbiologically induced corrosion of galvanized steel in the presence of Desulfovibrio sp. and Desulfosporosinus sp. were investigated. The corrosion behavior of galvanized steel was analyzed by potentiodynamic polarization and electrochemical impedance spectroscopy. The biofilm, corrosion products and Ag–Cu ions on the surfaces were investigated by using scanning electron microscopy, energy dispersive X-ray spectrometry and elemental mapping. The biofilm layer formed by the Desulfovibrio sp. was stable covering the all surface of galvanized steel coupons, while that by Desulfosporosinus sp. was intermittent, highly porous and heterogeneous. It was found that both of the sulfate reducing bacteria species accelerated corrosion of the galvanized steel. However, it was detected that Desulfosporosinus sp. was more corrosive for galvanized steel than Desulfovibrio sp. Scanning electron microscopy images showed that Desulfovibrio sp. and Desulfosporosinus sp. in biofilm clustered into patches on the galvanized steel surface when the culture contained toxic Ag–Cu ions. The ions affected the growth of the sulfate reducing bacteria strains in different ways and hence the corrosion behaviors. It was observed that the Ag–Cu ions affected negatively growth of Desulfosporosinus sp. especially after 24 h of exposure leading to a decrease in the corrosion rate of galvanized steel. However, Desulfovibrio sp. showed more corrosive effect in the presence of the ions according to the ions-free culture. Energy dispersive X-ray spectrometry analysis showed that corrosion products on the surfaces were mainly composed of Zn, S, Na, O and P. - Highlights: • Galvanized steel was corroded by Desulfosporosinus sp. and Desulfovibrio sp. • Desulfosporosinus sp. is more corrosive than Desulfovibrio sp. • The Ag–Cu ions affected corrosion behavior of Desulfosporosinus sp. and Desulfovibrio sp. on galvanized steel.

  6. In vitro cytotoxicity evaluation of nano-carbon particles with different sp{sup 2}/sp{sup 3} ratios

    Energy Technology Data Exchange (ETDEWEB)

    Li, S.S.; Wu, B.J.; Deng, Q.Y. [Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031 (China); Guo, Y.B. [The Third People' s Hospital of Chengdu, Sichuan 610031 (China); Leng, Y.X., E-mail: yxleng@263.net [Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031 (China); Huang, N. [Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031 (China)

    2017-06-01

    Graphitization occurs during the long-term service of a diamond-like carbon (DLC) modified artificial joint. Then, DLC wear debris, which are carbon particles with different sp{sup 2}/sp{sup 3} ratios and sizes ranging from the nano- to micro-meter scale produced. In this paper, to promote the application of DLC coating for artificial joint modification, the cytotoxicity of DLC debris (nano-carbon particles, NCs) with different sp{sup 2}/sp{sup 3} ratios was studied. The microstructure and physical characteristics of NCs with different sp{sup 2}/sp{sup 3} ratios were investigated by Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), Transmission Electron Microscope (TEM) and Dynamic Light Scattering (DLS). Meanwhile, osteoblasts and macrophages were applied to characterize the cytotoxicity of the NCs. In vitro cytotoxicity assay results indicated that cells incubated with NCs of different sp{sup 2}/sp{sup 3} ratios had greater osteogenic capacity, and these particles caused a weaker immune response in comparison with CoCrMo particles. Taken together, the results indicated that NCs with different sp{sup 2}/sp{sup 3} ratios presented a good cytocompatibility than CoCrMo particles. But no significant differences were observed among NCs with different sp{sup 2}/sp{sup 3} ratios. The better cytocompatibility of NCs is mainly attributable to their surface charge. - Highlights: • NCs with different sp{sup 2}/sp{sup 3} ratios have been successfully prepared by annealing treatment. • NCs with different sp{sup 2}/sp{sup 3} ratios show good osteogenic capacity and lower immune response. • The good cytocompatibility of NCs is mainly dependent on its surface charge.

  7. Microbiologically influenced corrosion of galvanized steel by Desulfovibrio sp. and Desulfosporosinus sp. in the presence of Ag–Cu ions

    International Nuclear Information System (INIS)

    Ilhan-Sungur, Esra; Unsal-Istek, Tuba; Cansever, Nurhan

    2015-01-01

    The effects of Ag–Cu ions on the microbiologically induced corrosion of galvanized steel in the presence of Desulfovibrio sp. and Desulfosporosinus sp. were investigated. The corrosion behavior of galvanized steel was analyzed by potentiodynamic polarization and electrochemical impedance spectroscopy. The biofilm, corrosion products and Ag–Cu ions on the surfaces were investigated by using scanning electron microscopy, energy dispersive X-ray spectrometry and elemental mapping. The biofilm layer formed by the Desulfovibrio sp. was stable covering the all surface of galvanized steel coupons, while that by Desulfosporosinus sp. was intermittent, highly porous and heterogeneous. It was found that both of the sulfate reducing bacteria species accelerated corrosion of the galvanized steel. However, it was detected that Desulfosporosinus sp. was more corrosive for galvanized steel than Desulfovibrio sp. Scanning electron microscopy images showed that Desulfovibrio sp. and Desulfosporosinus sp. in biofilm clustered into patches on the galvanized steel surface when the culture contained toxic Ag–Cu ions. The ions affected the growth of the sulfate reducing bacteria strains in different ways and hence the corrosion behaviors. It was observed that the Ag–Cu ions affected negatively growth of Desulfosporosinus sp. especially after 24 h of exposure leading to a decrease in the corrosion rate of galvanized steel. However, Desulfovibrio sp. showed more corrosive effect in the presence of the ions according to the ions-free culture. Energy dispersive X-ray spectrometry analysis showed that corrosion products on the surfaces were mainly composed of Zn, S, Na, O and P. - Highlights: • Galvanized steel was corroded by Desulfosporosinus sp. and Desulfovibrio sp. • Desulfosporosinus sp. is more corrosive than Desulfovibrio sp. • The Ag–Cu ions affected corrosion behavior of Desulfosporosinus sp. and Desulfovibrio sp. on galvanized steel

  8. Interaction of Sp1 zinc finger with transport factor in the nuclear localization of transcription factor Sp1

    International Nuclear Information System (INIS)

    Ito, Tatsuo; Kitamura, Haruka; Uwatoko, Chisana; Azumano, Makiko; Itoh, Kohji; Kuwahara, Jun

    2010-01-01

    Research highlights: → Sp1 zinc fingers themselves interact with importin α. → Sp1 zinc finger domains play an essential role as a nuclear localization signal. → Sp1 can be transported into the nucleus in an importin-dependent manner. -- Abstract: Transcription factor Sp1 is localized in the nucleus and regulates the expression of many cellular genes, but the nuclear transport mechanism of Sp1 is not well understood. In this study, we revealed that GST-fused Sp1 protein bound to endogenous importin α in HeLa cells via the Sp1 zinc finger domains, which comprise the DNA binding domain of Sp1. It was found that the Sp1 zinc finger domains directly interacted with a wide range of importin α including the armadillo (arm) repeat domain and the C-terminal acidic domain. Furthermore, it turned out that all three zinc fingers of Sp1 are essential for binding to importin α. Taken together, these results suggest that the Sp1 zinc finger domains play an essential role as a NLS and Sp1 can be transported into the nucleus in an importin-dependent manner even though it possesses no classical NLSs.

  9. A superhard sp3 microporous carbon with direct bandgap

    Science.gov (United States)

    Pan, Yilong; Xie, Chenlong; Xiong, Mei; Ma, Mengdong; Liu, Lingyu; Li, Zihe; Zhang, Shuangshuang; Gao, Guoying; Zhao, Zhisheng; Tian, Yongjun; Xu, Bo; He, Julong

    2017-12-01

    Carbon allotropes with distinct sp, sp2, and sp3 hybridization possess various different properties. Here, a novel all-sp3 hybridized tetragonal carbon, namely the P carbon, was predicted by the evolutionary particle swarm structural search. It demonstrated a low density among all-sp3 carbons, due to the corresponding distinctive microporous structure. P carbon is thermodynamically stable than the known C60 and could be formed through the single-walled carbon nanotubes (SWCNTs) compression. P carbon is a direct bandgap semiconductor displaying a strong and superhard nature. The unique combination of electrical and mechanical properties constitutes P carbon a potential superhard material for semiconductor industrial fields.

  10. Sp6 and Sp8 Transcription Factors Control AER Formation and Dorsal-Ventral Patterning in Limb Development

    Science.gov (United States)

    Haro, Endika; Delgado, Irene; Junco, Marisa; Yamada, Yoshihiko; Mansouri, Ahmed; Oberg, Kerby C.; Ros, Marian A.

    2014-01-01

    The formation and maintenance of the apical ectodermal ridge (AER) is critical for the outgrowth and patterning of the vertebrate limb. The induction of the AER is a complex process that relies on integrated interactions among the Fgf, Wnt, and Bmp signaling pathways that operate within the ectoderm and between the ectoderm and the mesoderm of the early limb bud. The transcription factors Sp6 and Sp8 are expressed in the limb ectoderm and AER during limb development. Sp6 mutant mice display a mild syndactyly phenotype while Sp8 mutants exhibit severe limb truncations. Both mutants show defects in AER maturation and in dorsal-ventral patterning. To gain further insights into the role Sp6 and Sp8 play in limb development, we have produced mice lacking both Sp6 and Sp8 activity in the limb ectoderm. Remarkably, the elimination or significant reduction in Sp6;Sp8 gene dosage leads to tetra-amelia; initial budding occurs, but neither Fgf8 nor En1 are activated. Mutants bearing a single functional allele of Sp8 (Sp6−/−;Sp8+/−) exhibit a split-hand/foot malformation phenotype with double dorsal digit tips probably due to an irregular and immature AER that is not maintained in the center of the bud and on the abnormal expansion of Wnt7a expression to the ventral ectoderm. Our data are compatible with Sp6 and Sp8 working together and in a dose-dependent manner as indispensable mediators of Wnt/βcatenin and Bmp signaling in the limb ectoderm. We suggest that the function of these factors links proximal-distal and dorsal-ventral patterning. PMID:25166858

  11. Analysis list: SP2 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SP2 Blood,Liver,Pluripotent stem cell + hg19 http://dbarchive.biosciencedbc.jp/kyus...hu-u/hg19/target/SP2.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/SP2.5.tsv http://dbarchive....biosciencedbc.jp/kyushu-u/hg19/target/SP2.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.Blood.tsv,http:...//dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.Liver.tsv,http:...//dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.Pluripotent_stem_cell.tsv http://dbarchive.biosciencedbc

  12. Biodegradation of resorcinol byPseudomonas sp.

    Institute of Scientific and Technical Information of China (English)

    Nader Hajizadeh; Najibeh Shirzad; Ali Farzi; Mojtaba Salouti; Azra Momeni

    2016-01-01

    ABSTRACT Objective:To investigate the ability ofPseudomonas sp. isolated from East Azarbaijan, Iran in bioremediation of resorcinol. Methods: Resorcinol biodegradation was evaluated using spectrophotometry and confirmed by gas chromatography-mass spectroscopy. Results:This isolate was able to remove up to 37.12% of resorcinol from contaminated water. Reusability experiments had confirmed the biodegradation process which produced seven intermediate compounds. These intermediates were characterized by gas chromatography-mass spectroscopy technique. The products of resorcinol biodegradation were apparently 1, 4-cyclohexadiene, nonadecene, 2-heptadecanone, 1-isopropyl-2-methoxy-4-methylbenzene, hexadecanoic acid, 9-octadecenoic acid, phenol and 5-methyl-2-(1-methylethyl). Conclusions: The findings revealed thatPseudomonas sp. is able to degrade resorcinol. Because of being an indigenous organism, this isolate is more compatible with the climate of the northwest region of Iran and possibly will be used for degradation of other similar aromatic compounds.

  13. SP-100 space reactor power system readiness

    International Nuclear Information System (INIS)

    Josloff, A.T.; Matteo, D.N.; Bailey, H.S.

    1992-01-01

    This paper discusses the SP-100 Space Reactor Power System which is being developed by GE, under contract to the U.S. Department of Energy, to provide electrical power in the range of 10's to 100's of kW. The system represents an enabling technology for a wide variety of earth orbital and interplanetary science missions, nuclear electric propulsion (NEP) stages, and lunar/Mars surface power for the Space Exploration Initiative (SEI). The technology and design is now at a state of readiness to support the definition of early flight demonstration missions. Of particular importance is that SP-100 meets the demanding U.S. safety performance, reliability and life requirements. The system is scalable and flexible and can be configured to provide 10's to 100's of kWe without repeating development work and can meet DoD goals for an early, low-power demonstration flight in the 1996-1997 time frame

  14. Superfield quantization in Sp(2) covariant formalism

    CERN Document Server

    Lavrov, P M

    2001-01-01

    The rules of the superfield Sp(2) covariant quantization of the arbitrary gauge theories for the case of the introduction of the gauging with the derivative equations for the gauge functional are generalized. The possibilities of realization of the expanded anti-brackets are considered and it is shown, that only one of the realizations is compatible with the transformations of the expanded BRST-symmetry in the form of super translations along the Grassmann superspace coordinates

  15. Spøgelsets egne ord

    DEFF Research Database (Denmark)

    Gemzøe, Anker

    2012-01-01

    refleksioner over den gengangerscene, der åbner Shakespeares Hamlet. Ifølge Derridas ’hantologie’ opererer spøgelser i en eksistentiel tidslighed, der har at gøre med sorg, sprog og forvandling. Genremæssigt, tilføjer Gemzøe, har gengangeren det med at husere i tragedien. Med Derridas ’hantologie’ som en af de...

  16. Carbon sp chains in graphene nanoholes

    International Nuclear Information System (INIS)

    Castelli, Ivano E; Ferri, Nicola; Onida, Giovanni; Manini, Nicola

    2012-01-01

    Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. We adopt a fair compromise of microscopic realism with a certain level of idealization in the model configurations, and predict a number of properties susceptible to comparison with experiment. (paper)

  17. Carbon sp chains in graphene nanoholes

    Science.gov (United States)

    Castelli, Ivano E.; Ferri, Nicola; Onida, Giovanni; Manini, Nicola

    2012-03-01

    Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. We adopt a fair compromise of microscopic realism with a certain level of idealization in the model configurations, and predict a number of properties susceptible to comparison with experiment.

  18. Commercial technologies from the SP-100 program

    International Nuclear Information System (INIS)

    Truscello, V.C.; Fujita, T.; Mondt, J.F.

    1995-01-01

    For more than a decade, Jet Propulsion Labortory and Los Alamos have managed a multi-agency funded effort to develop a space reactor power system. This SP-100 Program has developed technologies required for space power systems that can be implemented in the industrial and commercial sectors to improve competitiveness in the global economy. Initial steps taken to transfer this technology from the laboratories to industrial and commercial entities within United States include: (1) identifying specific technologies having commercial potential; (2) distributing information describing the identified technologies and interacting with interested commercial and industrial entities to develop application-specific details and requirements; and (3) providing a technological data base that leads to transfer of technology or the forming of teaming arrangements to accomplish the transfer by tailoring the technology to meet application-specific requirements. SP-100 technologies having commercial potential encompass fabrication processes, devices, and components. Examples: a process for bonding refractory metals to graphite, a device to sense the position of an actuator and a component to enable rotating machines to operate without supplying lubrication (self-lubricating ball bearing). Shortly after the NASA Regional Technology Transfer Centers widely disseminated information covering SP-100 technologies, over one hundred expressions of interest were received, which indicate that there is a large potential benefit in transferring SP-100 technology. Interactions with industrial and commercial entities have identified a substantial need for creating teaming arrangements involving the interested entity and personnel from laboratories and their contractors, who have the knowledge and ability to tailor the technology to meet application-specific requirements. copyright 1995 American Institute of Physics

  19. Thermomucor abortosporangium sp. nov. (Fungi: Mucorales

    Directory of Open Access Journals (Sweden)

    A. Swathi Sri

    2012-03-01

    Full Text Available Thermomucor abortosporangium A. Swathi Sri & A. Subrahm., sp. nov. is described as the second species of Thermomucor. It differs from the type species of T. indicae-seudaticae in the branching pattern of sporangiophores, size and shape of sporangiospores and production of abortosporangia. Zygospores are reddish brown, globose, psilate, isogamic and formed at low (37°C temperature whilst the higher temperatures are prohibitive. The fungus grows well at 50°C on PDA, SMA and MEA.

  20. Optimization of cellulase production by Penicillium sp.

    Science.gov (United States)

    Prasanna, H N; Ramanjaneyulu, G; Rajasekhar Reddy, B

    2016-12-01

    The production of cellulolytic enzymes (β-exoglucanase, β-endoglucanase and β-glucosidase) by Penicillium sp. on three different media in liquid shake culture conditions was compared. The organism exhibited relatively highest activity of endoglucanase among three enzymes measured at 7-day interval during the course of its growth on Czapek-Dox medium supplemented with 0.5 % (w/v) cellulose. Cellulose at 0.5 %, lactose at 0.5 %, sawdust at 0.5 %, yeast extract at 0.2 % as a nitrogen source, pH 5.0 and 30 °C temperature were found to be optimal for growth and cellulase production by Penicillium sp. Yields of Fpase, CMCase and β-glucosidase, attained on optimized medium with Penicillium sp. were 8.7, 25 and 9.52 U/ml, respectively with increment of 9.2, 5.9 and 43.8-folds over titers of the respective enzyme on unoptimised medium. Cellulase of the fungal culture with the ratio of β-glucosidase to Fpase greater than one will hold potential for biotechnological applications.

  1. Biosorption of 241Am by Candida sp

    International Nuclear Information System (INIS)

    Luo Shunzhong; Zhang Taiming; Liu Ning; Yang Yuanyou; Jin Jiannan; Hua Xinfeng

    2003-01-01

    The biosorption of radionuclide 241 Am from solutions by Candida sp., and the influences of experimental conditions on the adsorption were studied. The results showed that the adsorption equilibrium was achieved within 4h and the optimum pH=2. No significant differences on 241 Am biosorption were observed at 10-45 degree C, or challenged with Au 3+ or Ag + , even 1500 times or 4500 times over 241 Am, respectively. The adsorption rate could reach 97.8% by dry Candida sp. of 0.82 g/L in 241 Am solutions (pH=2) of 5.6-111 MBq/L (44.04-873.0 μg/L) (C 0 ), with maximum adsorption capacity (W) of 63.5 MBq/g (501.8 μg/g), implying that the removal of 241 Am by Candida sp. from solutions was feasible. The relationship between activities (C 0 ) and adsorption capacities (W) of 241 Am indicated that the biosorption process could be described by Langmuir adsorption isotherm

  2. Biosorption of americium-241 by Candida sp

    International Nuclear Information System (INIS)

    Luo Shunzhong; Zhang Taiming; Liu Ning; Yang Yuanyou; Jin Jiannan; Liao Jiali

    2003-01-01

    As an important radioisotope in nuclear industry and other fields, americium-241 is one of the most serious contamination concerns duo to its high toxicity and long half-life. In this experiment, the biosorption of 241 Am from solution by Candida sp., and the effects of various experimental conditions on the adsorption were investigated. The preliminary results showed that the adsorption of 241 Am by Candida sp. was efficient. 241 Am could be removed by Candida sp. of 0.82 g/L (dry weight) from 241 Am solutions of 5.6-111 MBq/L (44.3-877.2 μg/L)(C 0 ), with maximum adsorption rate (R) of 98% and maximum adsorption capacities (W) of 63.5 MBq/g biomass (dry weight) (501.8 μg/g). The biosorption equilibrium was achieved within 4 hour and the optimum pH was pH = 2. No significant differences on 241 Am adsorption were observed at 10 C-45 C, or in solutions containing Au 3+ or Ag + , even 1500 times or 4500 times above the 241 Am concentration, respectively. The relationship between concentrations and adsorption capacities of 241 Am indicated the biosorption process should be described by a Langmuir adsorption isotherm. (orig.)

  3. Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov. prevail in diverse enzootic transmission cycles.

    Science.gov (United States)

    Margos, Gabriele; Lane, Robert S; Fedorova, Natalia; Koloczek, Johannes; Piesman, Joseph; Hojgaard, Andrias; Sing, Andreas; Fingerle, Volker

    2016-03-01

    Two species of the genus Borrelia , Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov., were first described by Postic and co-workers on the basis of genetic analyses of several loci. Multilocus sequence analysis of eight housekeeping loci confirmed that these two Borrelia genomospecies are distinct members of the Borrelia burgdorferi sensu lato complex. B. bissettiae sp. nov. was initially described in transmission cycles involving Neotoma fuscipes wood rats and Ixodes pacificus ticks in California, and Neotoma mexicana and Ixodes spinipalpis in Colorado. The preferred host of B. californiensis sp. nov. appears to be the California kangaroo rat, Dipodomys californicus ; Ixodes jellisoni , I. spinipalipis and I. pacificus ticks are naturally infected with it. Thus, the ecological associations of the two genomospecies and their genetic distance from all other known Borrelia genomospecies species justify their description as separate genomospecies: B. bissettiae sp. nov. (type strain DN127 T  = DSM 17990 T  =  CIP 109136 T ) and B. californiensis (type strain CA446 T  = DSM 17989 T  = ATCC BAA-2689 T ).

  4. EFEKTIFITAS DAYA HAMBAT BAKTERI Streptomyces sp TERHADAP Erwinia sp PENYEBAB PENYAKIT BUSUK REBAH PADA TANAMAN LIDAH BUAYA (Aloe barbadensis Mill

    Directory of Open Access Journals (Sweden)

    SARMILA TASNIM

    2013-05-01

    Full Text Available Streptomyces sp was conducted from December 2010 - June 2011 at the Laboratoryof Microbiology, Biology Department, Math and Science Faculty, UdayanaUniversity Bukit Jimbaran-Bali. Implementation stages of the research consisted ofisolation and testing of the antibiotic activity Streptomyces sp to inhibit growthbacterial pathogens Erwinia sp as a cause of disease in plants fallen foul (Soft rot ofAloe barbadensis Mill.The results of this study have eight isolates of Streptomyces spwith macroscopic and microscopic characters are varied. Furthermore, all isolateswere obtained and then tested against antibiotic activity to inhibit growth the bacteriaErwinia sp. Test results obtained by Streptomyces sp that has the most effective ininhibiting the ability of the bacteria Erwinia sp isolates are Streptomyces sp2for (45%.

  5. Nanoscale transformation of sp2 to sp3 of graphite by slow highly charged ion irradiation

    International Nuclear Information System (INIS)

    Meguro, T.; Hida, A.; Koguchi, Y.; Miyamoto, S.; Yamamoto, Y.; Takai, H.; Maeda, K.; Aoyagi, Y.

    2003-01-01

    Nanoscale transformation of electronic states by highly charged ion (HCI) impact on graphite surfaces is described. The high potential energy of slow HCI, which induces multiple emission of electrons from the surface, provides a strong modification of the electronic states of the local area upon graphite surfaces. The HCI impact and the subsequent surface treatment either by electron injection from a scanning tunneling microscopy tip or by He-Cd laser irradiation induce a localized transition from sp 2 to sp 3 hybridization in graphite, resulting in the formation of nanoscale diamond-like structures (nanodiamond) at the impact region. From Raman spectroscopic measurements on sp 2 related peaks, it is found that the HCI irradiation creates vacancy complexes in contrast to ions having a lower charge state, which generate single vacancies. It is of interest that a single impact of HCI creates one nanodiamond structure, suggesting potential applications of HCI in nanoscale material processing

  6. Burkholderia humisilvae sp. nov., Burkholderia solisilvae sp. nov. and Burkholderia rhizosphaerae sp. nov., isolated from forest soil and rhizosphere soil.

    Science.gov (United States)

    Lee, Jae-Chan; Whang, Kyung-Sook

    2015-09-01

    Strains Y-12(T) and Y-47(T) were isolated from mountain forest soil and strain WR43(T) was isolated from rhizosphere soil, at Daejeon, Korea. The three strains grew at 10-55 °C (optimal growth at 28-30 °C), at pH 3.0-8.0 (optimal growth at pH 6.0) and in the presence of 0-4.0% (w/v) NaCl, growing optimally in the absence of added NaCl. On the basis of 16S rRNA gene sequence analysis, the three strains were found to belong to the genus Burkholderia, showing the closest phylogenetic similarity to Burkholderia diazotrophica JPY461(T) (97.2-97.7%); the similarity between the three sequences ranged from 98.3 to 98.7%. Additionally, the three strains formed a distinct group in phylogenetic trees based on the housekeeping genes recA and gyrB. The predominant ubiquinone was Q-8, the major fatty acids were C16 : 0 and C17  : 0 cyclo and the DNA G+C content of the novel isolates was 61.6-64.4 mol%. DNA-DNA relatedness among the three strains and the type strains of the closest species of the genus Burkholderia was less than 50%. On the basis of 16S rRNA, recA and gyrB gene sequence similarities, chemotaxonomic and phenotypic data, the three strains represent three novel species within the genus Burkholderia, for which the names Burkholderia humisilvae sp. nov. (type strain Y-12(T)= KACC 17601(T) = NBRC 109933(T) = NCAIM B 02543(T)), Burkholderia solisilvae sp. nov. (type strain Y-47(T) = KACC 17602(T)= NBRC 109934(T) = NCAIM B 02539(T)) and Burkholderia rhizosphaerae sp. nov. (type strain WR43(T) = KACC 17603(T) = NBRC 109935(T) = NCAIM B 02541(T)) are proposed.

  7. Bifidobacterium reuteri sp. nov., Bifidobacterium callitrichos sp. nov., Bifidobacterium saguini sp. nov., Bifidobacterium stellenboschense sp. nov. and Bifidobacterium biavatii sp. nov. isolated from faeces of common marmoset (Callithrix jacchus) and red-handed tamarin (Saguinus midas).

    Science.gov (United States)

    Endo, Akihito; Futagawa-Endo, Yuka; Schumann, Peter; Pukall, Rüdiger; Dicks, Leon M T

    2012-03-01

    Five strains of bifidobacteria were isolated from faeces of a common marmoset (Callithrix jacchus) and a red-handed tamarin (Saguinus midas). The five isolates clustered inside the phylogenetic group of the genus Bifidobacterium but did not show high sequence similarities between the isolates and to known species in the genus by phylogenetic analysis based on 16S rRNA gene sequences. Sequence analyses of dnaJ1 and hsp60 also indicated their independent phylogenetic positions to each other in the Bifidobacterium cluster. DNA G+C contents of the species ranged from 57.3 to 66.3 mol%, which is within the values recorded for Bifidobacterium species. All isolates showed fructose-6-phosphate phosphoketolase activity. Based on the data provided, the five isolates represent five novel species, for which the names Bifidobacterium reuteri sp. nov. (type strain: AFB22-1(T) = JCM 17295(T) = DSM 23975(T)), Bifidobacterium callitrichos sp. nov. (type strain: AFB22-5(T) = JCM 17296(T) = DSM 23973(T)), Bifidobacterium saguini sp. nov. (type strain: AFB23-1(T) = JCM 17297(T) = DSM 23967(T)), Bifidobacterium stellenboschense sp. nov. (type strain: AFB23-3(T) = JCM 17298(T) = DSM 23968(T)) and Bifidobacterium biavatii sp. nov. (type strain: AFB23-4(T) = JCM 17299(T) = DSM 23969(T)) are proposed. Copyright © 2011 Elsevier GmbH. All rights reserved.

  8. Fermentation of Azolla sp. leaves and the utilization as a feed ingredient of tilapia Oreochromis sp.

    Directory of Open Access Journals (Sweden)

    Nur Bambang Priyo Utomo

    2015-03-01

    Full Text Available ABSTRACTThe objective of this study was to study the effect of incubation period on the nutritional content of Azolla sp. meal fermented by Trichoderma harzianum, and its optimum supplementation level in the feed of tilapia Oreochromis sp. In incubation period treatments, fermentation of Azolla meal was performed in two, six, eight, and ten days (AF2, AF6, AF8, AF10 using Trichoderma harzianum as the fermentor. The fish used in this study was tilapia Oreochromis sp. with an average weight of 10.59±1.29 g. The design of the feeding treatments was repeletting commercial feed with Azolla leaves by with different supplementation levels, i.e. 0% (A/control, 30% (B, 60% (C, and 90% (D. Faecal collection for digestibility measurement was conducted for 15 days and fish growth rate was observed for 40 days. Azolla meal fermented for two days (AF2 showed the best results among the other treatments with a crude fiber decrease of 37.19% and protein increase of 38.65%. The results of this study indicate that fermentation can increase the nutritional quality of Azolla meal and its most optimal supplementation level in the diet of tilapia is 30%.Keywords: crude fiber, Azolla sp., tilapiaABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh lama waktu fermentasi daun mata lele Azolla sp. menggunakan kapang Trichoderma harzianum serta dosis optimal dalam pakan ikan nila Oreochromis sp. Proses fermentasi tepung daun mata lele dilakukan selama dua, enam, delapan, dan sepuluh hari (AF2, AF6, AF8, AF10. Ikan uji pada penelitian ini menggunakan ikan nila Oreochromis sp. dengan bobot rata-rata 10,59±1,29 g yang ditebar sebanyak 6 ekor/akuarium berukuran 50×45×30 cm3. Sebagai pakan perlakuan yakni repeletting daun mata lele dengan pakan komersil pada tingkat suplementasi 0% (A/kontrol, 30% (B, 60% (C, dan 90% (D. Pemeliharaan ikan uji dan pengumpulan data dilakukan dengan mengumpulkan feses ikan untuk uji ketercernaan selama 15 hari dan mengamati pertumbuhan

  9. Isolation of C11 Cyclopentenones from Two Didemnid Species, Lissoclinum sp. and Diplosoma sp.

    Directory of Open Access Journals (Sweden)

    Katsuhiro Ueda

    2009-12-01

    Full Text Available A series of new C11 cyclopentenones 1-7 was isolated, together with four known metabolites 9/10, 12 and 13, from the extract of the didemnid ascidian Lissoclinum sp. The other didemnid ascidian Diplosoma sp. contained didemnenones 1, 2 and 5, and five known metabolites 8-12. The structures of 1-7 were elucidated by spectroscopic analyses. Cytotoxicity of the isolated compounds was evaluated against three human cancer cell lines (HCT116, A431 and A549.

  10. Biomass accumulation and chemical composition of Massai grass intercropped with forage legumes on an integrated crop-livestock-forest system

    Directory of Open Access Journals (Sweden)

    Tatiana da Costa Moreno Gama

    2014-06-01

    Full Text Available The objective was to evaluate the use of woody legumes (Albizia lebbeck, Cratylia argentea, Dipteryx Allata (Baru, a Leucaena hybrid (L. leucocephala + L. diversifolia, and Leucaena leucocephalacv. Cunningham and herbaceous legumes (Arachis pintoi intercropped with Panicum maximum cv. Massai, simultaneously implanted in a maize crop. The study made use of a randomized block experimental design with four replications. Assessments of biomass accumulation and forage nutritional value were made after the maize harvest, between June 2008 and October 2010. It was found that the residues of maize provided better growing conditions for Massai grass during the dry season. L. leucocephala cv. Cunningham and the Leucaena hybrid had the highest accumulation of all forage legumes evaluated, and provided the best nutritional value of all the arrangements tested. Of all woody legumes tested in this system, Leucaena was considered feasible for intercropping with Massai grass. The intercrop of perennial woody Baru with maize is not recommended. Albizia lebbeck and Cratylia argentea require further study, especially the yield assessment at different cutting intervals and cutting heights. Arachis pintoi had a low participation in the intercropping, showing greater performance over time, indicating slow thriving in this experimental condition.

  11. Role of surfactant protein A (SP-A)/lipid interactions for SP-A functions in the lung.

    Science.gov (United States)

    Casals, C

    2001-01-01

    Surfactant protein A (SP-A), an oligomeric glycoprotein, is a member of a group of proteins named collectins that contain collagen-like and Ca(2+)-dependent carbohydrate recognition domains. SP-A interacts with a broad range of amphipathic lipids (glycerophospholipids, sphingophospholipids, glycosphingolipids, lipid A, and lipoglycans) that are present in surfactant or microbial membranes. This review summarizes SP-A/lipid interaction studies regarding the lipid system used (i.e., phospholipid vesicles, phospholipid monolayers, and lipids immobilized on silica or adsorbed on a solid support). The effect of calcium, ionic strength, and pH on the binding of SP-A to lipids and the subsequent lipid aggregation process is discussed. Current evidence suggests that hydrophobic-binding forces are involved in the peripherical association of SP-A to membranes. It is also proposed that fluid and liquid-ordered phase coexistence in surfactant membranes might favor partition of SP-A into those membranes. The binding of SP-A to surfactant membranes containing hydrophobic surfactant peptides makes possible the formation of a membrane reservoir in the alveolar fluid that is protected by SP-A against inactivation and improves the rate of surfactant film formation. In addition, the interaction of SP-A with membranes might enhance the affinity of SP-A for terminal carbohydrates of glycolipids or glycoproteins on the surface of invading microorganisms.

  12. Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource.

    Directory of Open Access Journals (Sweden)

    Akanksha Srivastava

    Full Text Available An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732 were isolated (paddy fields and ponds in the Banaras Hindu University, campus and five strains screened for anticancer potential using human colon adenocarcinoma (HT29 and human kidney adenocarcinoma (A498 cancer cell lines. Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 were the most potent as determined by examination of morphological features and by inhibition of growth by graded concentrations of crude extracts and thin-layer chromatography (TLC eluates. Cell cycle analysis and multiplex assays using cancer biomarkers also confirmed Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as cancer drug resources. Apoptotic studies in the cells of A498 (cancer and MCF-10A (normal human epithelial exposed to crude extracts and TLC fractions revealed no significant impact on MCF-10A cells emphasizing its importance in the development of anticancer drug. Identification of biomolecules from these extracts are in progress.

  13. Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource.

    Science.gov (United States)

    Srivastava, Akanksha; Tiwari, Ratnakar; Srivastava, Vikas; Singh, Tej Bali; Asthana, Ravi Kumar

    2015-01-01

    An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732) were isolated (paddy fields and ponds in the Banaras Hindu University, campus) and five strains screened for anticancer potential using human colon adenocarcinoma (HT29) and human kidney adenocarcinoma (A498) cancer cell lines. Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 were the most potent as determined by examination of morphological features and by inhibition of growth by graded concentrations of crude extracts and thin-layer chromatography (TLC) eluates. Cell cycle analysis and multiplex assays using cancer biomarkers also confirmed Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as cancer drug resources. Apoptotic studies in the cells of A498 (cancer) and MCF-10A (normal human epithelial) exposed to crude extracts and TLC fractions revealed no significant impact on MCF-10A cells emphasizing its importance in the development of anticancer drug. Identification of biomolecules from these extracts are in progress.

  14. Dominancy of Trichodesmium sp. in the Biawak Island

    Science.gov (United States)

    Prihadi, D. J.

    2018-03-01

    The Biawak Island is one of the small islands in West Java Province with an abundance of marine biological resources. This research was conducted to collect the primary producer zooplankton and water quality parameters. Direct observation is done by field surveys and measurement in situ for plankton and environmental parameters such as temperature, water transparency, water current, salinity, dissolved oxygen, and pH. Trichodesmium sp. was found dominance in where some other types of zooplankton were found in the area, like Scenedesmus sp., Sagitta sp., Acartia sp. also occurred. Further, the most abundance of Trichodesmium sp. was found in southern of Biawak Island where mangroves, coral and seagrass ecosystem provide nutrients which indirectly support the abundance of planktons. Trichodesmium sp. is plankton that can survive in water with minimum nutrient.

  15. Green Decision Making: Sustainable Transport and Systemic Planning (SP)

    DEFF Research Database (Denmark)

    Leleur, Steen

    and infrastructure assessment and on the methodology and process of systemic planning (SP). SP theory development has interchanged with practical application and testing of the SP approach in a large number of cases. The word systemic in SP indicates that complex planning problems and provision of decision support...... in today’s strategic planning needs a focus on what may be addressed as systemic insights in balance with more conventional, systematically-based findings where causal linkages can be modelled and made use of. In practice this means that SP is based on a study-specific combination of hard (quantitative......) and soft (qualitative) operations research (OR) methods; especially the latter have a function as regards knowledge generation that relates to obtaining systemic insights. Furthermore, SP applies a process that drives group-based learning forward. The group should be formed with the different stakeholder...

  16. The SP Theory of Intelligence: An Overview

    Directory of Open Access Journals (Sweden)

    J Gerard Wolff

    2013-08-01

    Full Text Available This article is an overview of the SP theory of intelligence, which aims to simplify and integrate concepts across artificial intelligence, mainstream computing and human perception and cognition, with information compression as a unifying theme. It is conceived of as a brain-like system that receives "New" information and stores some or all of it in compressed form as "Old" information; and it is realised in the form of a computer model, a first version of the SP machine. The matching and unification of patterns and the concept of multiple alignment are central ideas. Using heuristic techniques, the system builds multiple alignments that are "good" in terms of information compression. For each multiple alignment, probabilities may be calculated for associated inferences. Unsupervised learning is done by deriving new structures from partial matches between patterns and via heuristic search for sets of structures that are "good" in terms of information compression. These are normally ones that people judge to be "natural", in accordance with the "DONSVIC" principle—the discovery of natural structures via information compression. The SP theory provides an interpretation for concepts and phenomena in several other areas, including "computing", aspects of mathematics and logic, the representation of knowledge, natural language processing, pattern recognition, several kinds of reasoning, information storage and retrieval, planning and problem solving, information compression, neuroscience and human perception and cognition. Examples include the parsing and production of language with discontinuous dependencies in syntax, pattern recognition at multiple levels of abstraction and its integration with part-whole relations, nonmonotonic reasoning and reasoning with default values, reasoning in Bayesian networks, including "explaining away", causal diagnosis, and the solving of a geometric analogy problem.

  17. Methyl (Sp-2-(diphenylphosphinoferrocene-1-carboxylate

    Directory of Open Access Journals (Sweden)

    Petr Štěpnička

    2009-10-01

    Full Text Available The title compound, [Fe(C5H5(C19H16O2P], obtained serendipitously during recrystallization of 1-hydroxybenzotriazolyl (Sp-2-(diphenylphosphinoferrocene-1-carboxylate from methanol, crystallizes in the chiral space group P212121. Its crystal structure not only confirms the anticipated absolute configuration but also establishes a rather regular geometry for the ferrocene unit, devoid of any significant deformation due to the attached substituents. In the crystal, symmetry-related molecules are linked via weak C—H...O interactions.

  18. Increasing rice plant growth by Trichoderma sp.

    Science.gov (United States)

    Doni, Febri; Isahak, Anizan; Zain, Che Radziah Che Mohd; Sulaiman, Norela; Fathurahman, F.; Zain, Wan Nur Syazana Wan Mohd.; Kadhimi, Ahsan A.; Alhasnawi, Arshad Naji; Anhar, Azwir; Yusoff, Wan Mohtar Wan

    2016-11-01

    Trichoderma sp. is a plant growth promoting fungi in many crops. Initial observation on the ability to enhance rice germination and vigor have been reported. In this study, the effectiveness of a local isolate Trichoderma asprellum SL2 to enhance rice seedling growth was assessed experimentally under greenhouse condition using a completely randomized design. Results showed that inoculation of rice plants with Trichoderma asprellum SL2 significantly increase rice plants height, root length, wet weight, leaf number and biomass compared to untreated rice plants (control). The result of this study can serve as a reference for further work on the application of beneficial microorganisms to enhance rice production.

  19. SP-100 radiator design trade study

    International Nuclear Information System (INIS)

    Ewell, R.

    1992-01-01

    This paper reports on a design trade study of the SP-100 heat rejection subsystem (HRSS) which was made. A system code was used to evaluate the sensitivity of the HRSS mass and performance to changes. Variations in heat pipe diameter and cross-section, fin length and thickness, armor thickness, and overall configuration and materials were evaluated. The analysis indicates that the minimum system mass occurs for the case with many small diameter heat pipes, with ducting that maximizes the fraction of the heat pipe evaporator perimeter in contract with it

  20. Prepare for an SpR interview.

    Science.gov (United States)

    Garner, J P; Heppell, P S J

    2003-03-01

    By the time you attend an interview for a military SpR number you should have no real problems but it pays to be prepared. Begin preparations early, reading widely and talk to as many people as possible. Your consultants will have a useful viewpoint on the proceedings and may be able to help you refine your answers to the common questions. Arrive at your interview in a smart and timely fashion and answer questions with confidence and common sense. Avoid confrontation and bluff and be courteous at all times, whatever you may be feeling inside and thank the interview panel as you leave.

  1. Amino Alcohols from the Ascidian Pseudodistoma sp.

    Directory of Open Access Journals (Sweden)

    Tae Hyung Won

    2014-06-01

    Full Text Available Seven new amino alcohol compounds, pseudoaminols A–G (1–7, were isolated from the ascidian Pseudodistoma sp. collected off the coast of Chuja-do, Korea. Structures of these new compounds were determined by analysis of the spectroscopic data and from chemical conversion. The presence of an N-carboxymethyl group in two of the new compounds (6 and 7 is unprecedented among amino alcohols. Several of these compounds exhibited moderate antimicrobial activity and cytotoxicity, as well as weak inhibitory activity toward Na+/K+-ATPase.

  2. PRODUCTION OF FIBRINOLYTIC ENZYME (NATTOKINASE) FROM BACILLUS SP.

    OpenAIRE

    Padma Singh, Rekha Negi*, Vani Sharma, Alka Rani, Pallavi and Richa Prasad

    2018-01-01

    During present study Nattokinase which is a novel fibrinolytic enzyme was produced by Bacillus sp. To screen and extract nattokinase enzyme from Bacillus sp. were isolated from soil of different agricultural field by serial dilution method. Out of 10 isolate, one strain i.e. B3 produced nattokinase on screening medium. B3 was identified by biochemical characterization. The caseinolytic activity of Nattokinase was 0.526 U/ml and the selected isolate Bacillus sp. could produce active nattokinas...

  3. Indirect Manganese Removal by Stenotrophomonas sp. and Lysinibacillus sp. Isolated from Brazilian Mine Water

    Directory of Open Access Journals (Sweden)

    Natália Rocha Barboza

    2015-01-01

    Full Text Available Manganese is a contaminant in the wastewaters produced by Brazilian mining operations, and the removal of the metal is notoriously difficult because of the high stability of the Mn(II ion in aqueous solutions. To explore a biological approach for removing excessive amounts of aqueous Mn(II, we investigated the potential of Mn(II oxidation by both consortium and bacterial isolates from a Brazilian manganese mine. A bacterial consortium was able to remove 99.7% of the Mn(II. A phylogenetic analysis of isolates demonstrated that the predominant microorganisms were members of Stenotrophomonas, Bacillus, and Lysinibacillus genera. Mn(II removal rates between 58.5% and 70.9% were observed for Bacillus sp. and Stenotrophomonas sp. while the Lysinibacillus isolate 13P removes 82.7%. The catalytic oxidation of Mn(II mediated by multicopper oxidase was not properly detected; however, in all of the experiments, a significant increase in the pH of the culture medium was detected. No aggregates inside the cells grown for a week were found by electronic microscopy. Nevertheless, an energy-dispersive X-ray spectroscopy of the isolates revealed the presence of manganese in Stenotrophomonas sp. and Lysinibacillus sp. grown in K medium. These results suggest that members of Stenotrophomonas and Lysinibacillus genera were able to remove Mn(II by a nonenzymatic pathway.

  4. Screening of biodiesel production from waste tuna oil (Thunnus sp.), seaweed Kappaphycus alvarezii and Gracilaria sp.

    Science.gov (United States)

    Alamsjah, Mochammad Amin; Abdillah, Annur Ahadi; Mustikawati, Hutami; Atari, Suci Dwi Purnawa

    2017-09-01

    Biodiesel has several advantages over solar. Compared to solar, biodiesel has more eco-friendly characteristic and produces lower greenhouse gas emissions. Biodiesel that is made from animal fats can be produced from fish oil, while other alternative sources from vegetable oils are seaweed Kappaphycus alvarezii and Gracilaria sp. Waste tuna oil (Thunnus sp.) in Indonesia is commonly a side product of tuna canning industries known as tuna precook oil; on the other hand, seaweed Gracilaria sp. and Kappaphycus alvarezii are commonly found in Indonesia's seas. Seaweed waste that was used in the present study was 100 kg and in wet condition, and the waste oil was 10 liter. The seaweed was extracted with soxhletation method that used n-hexane as the solvent. To produce biodiesel, trans esterification was performed on the seaweed oil that was obtained from the soxhletation process and waste tuna oil. Biodiesel manufactured from seaweed K. alvarezii obtained the best score in flash point, freezing point, and viscosity test. However, according to level of manufacturing efficiency, biodiesel from waste tuna oil is more efficient and relatively easier compared to biodiesel from waste K. alvarezii and Gracilaria sp.

  5. Biodegradation of hexavalent chromium (Cr+6) in wastewater using Pseudomonas sp. and Bacillus sp. bacterial strains

    Energy Technology Data Exchange (ETDEWEB)

    Qasim, Muhammad [Department of Chemical Engineering, American University of Sharjah (United Arab Emirates)

    2013-07-01

    The recovery of toxic metal compounds is a deep concern in all industries. Hexavalent chromium is particularly worrying because of its toxic influence on human health. In this paper, biodegradation of hexavalent chromium (Cr+6) present in wastewater has been studied using two different bacterial strains; Pseudomonas sp. and Bacillus sp. A chemostat (with and without recycle of cells) with 10 L liquid culture volume was used to study the substrate and the biomass cell concentrations with time. Also, the degree of substrate conversion was studied by the varying the dilution rate as an independent parameter. The dilution rate (ratio of feed flow rate to the culture volume) was varied by varying the feed volumetric rate from 110-170 mL/h for inlet hexavalent chromium concentrations of 70 mg/dm3. The results show that a chemostat with recycle gives a better performance in terms of substrate conversion than a chemostat without a recycle. Moreover, the degree of substrate conversion decreases as the dilution rate is increased. Also, Bacillus sp. was found to give higher conversions compared to pseudomonas sp.

  6. Genetic diversity in Fusarium oxysporum f.sp. dianthi and F. redolens f.sp. dianthi

    NARCIS (Netherlands)

    Baayen, R.P.; Dreven, van F.; Krijger, M.C.; Waalwijk, C.

    1997-01-01

    Pathogenic isolates were selected representing all known vegetative compatibility groups (VCGs) and races of Fusarium oxysporum sensu lato from Dianthus spp. On basis of differences in the internal transcribed spacer region of the ribosomal DNA, six VCGs were classified as F. oxysporum f.sp. dianthi

  7. SHEAR STRENGTH IN THE GLUE LINE OF Eucalyptus sp. AND Pinus sp.WOOD

    Directory of Open Access Journals (Sweden)

    Juliana Jerásio Bianche

    Full Text Available ABSTRACT To evaluate the adhesive efficiency on the union of glued joints in a particular temperature and humidity conditions for a specified time the adhesive must be submitted to specific load tests, such as shear in the glue line. The objective of this study was to evaluate the shear strength in the glue line of Eucalyptus sp and Pinus sp.woods. Five adhesives (castor oil, sodium silicate, modified silicate, , PVA and resorcinol-formaldehyde, three weights (150 g/m2, 200 g/m2, and 250 g/m2 and two species (Eucalyptus sp. and Pinus sp. of wood were used. Twelve specimens were obtained from each repetition per treatment, corresponding to 108 specimens that were conditioned at a temperature of 23 ± 1°C and relative humidity of 50 ± 2%. The interaction between the weight and type of adhesive was significant for the shear strength in the glue line of eucalyptus wood. However, no interaction between the weight and the adhesive was found for pinus, only the isolated from the adhesive effect. Chemical bonds originated in the polymerization of resorcinol-formaldehyde adhesives and castor bi-component conferred upon these adhesives the greatest resistance in the glue line. Castor and resorcinol-formaldehyde adhesives showed the highest shear strength values in the line of glue and wood failure. Castor adhesive presented satisfactory performance for bonding of eucalyptus and pine woods.

  8. Mineralization of a Malaysian crude oil by Pseudomonas sp. and Achromabacter sp. isolated from coastal waters

    Energy Technology Data Exchange (ETDEWEB)

    Ahmad, J.; Ahmad, M.F.

    1995-12-31

    Regarded as being a potentially effective tool to combat oil pollution, bioremediation involves mineralization, i.e., the conversion of complex hydrocarbons into harmless CO{sub 2} and water by action of microorganisms. Therefore, in achieving optimum effectiveness from the application of these products on crude oil in local environments, the capability of the bacteria to mineralize hydrocarbons was evaluated. The microbial laboratory testing of mineralization on local oil degraders involved, first, isolation of bacteria found at a port located on the west coast of Peninsular Malaysia. Subsequently, these bacteria were identified by means of Biomereux`s API 20E and 20 NE systems and later screened by their growth on a Malaysian crude oil. Selected strains of Pseudomonas sp. and Achromabacter sp. were then exposed individually to a similar crude oil in a mineralization unit and monitored for 16 days for release of CO{sub 2}. Pseudomonas paucimobilis was found to produce more CO{sub 2} than Achromobacter sp. When tested under similar conditions, mixed populations of these two taxa produced more CO{sub 2} than that produced by any individual strain. Effective bioremediation of local crude in Malaysian waters can therefore be achieved from biochemically developed Pseudomonas sp. strains.

  9. SP@CE - An SP-based programming model for consumer electronics streaming applications

    NARCIS (Netherlands)

    Varbanescu, Ana Lucia; Nijhuis, Maik; Escribano, Arturo González; Sips, Henk; Bos, Herbert; Bal, Henri

    2007-01-01

    Efficient programming of multimedia streaming applications for Consumer Electronics (CE) devices is not trivial. As a solution for this problem, we present SP@CE, a novel programming model designed to balance the specific requirements of CE streaming applications with the simplicity and efficiency

  10. Eficiencia de pseudomonas sp, rhodopseudomonas sp, micrococcus sp y bacillus sp empleados como cultivos individuales y en consorcio, en la degradación de petróleo diesel ii

    OpenAIRE

    Otiniano García, Nélida Milly Esther

    2010-01-01

    In order to evaluate the efficiency of Pseudomonas sp, Rhodopseudomonas sp, Micrococcus sp, Bacillus sp, and the consortium formed by these four microorganisms in the diesel II petroleum degradation, it was worked in 5 bioreactors of aerated and shaken tank of 1.5 litters of capacity, with speed agitation of 120 rpm, and air flow of 0.5 vvm; in which were placed; 940 mL of Minimum Broth of Davies pH 7.0; 50 mL of diesel II petroleum as source of carbon and 10 mL of a suspension of approx...

  11. Sp(2) covariant quantisation of general gauge theories

    Energy Technology Data Exchange (ETDEWEB)

    Vazquez-Bello, J L

    1994-11-01

    The Sp(2) covariant quantization of gauge theories is studied. The geometrical interpretation of gauge theories in terms of quasi principal fibre bundles Q(M{sub s}, G{sub s}) is reviewed. It is then described the Sp(2) algebra of ordinary Yang-Mills theory. A consistent formulation of covariant Lagrangian quantisation for general gauge theories based on Sp(2) BRST symmetry is established. The original N = 1, ten dimensional superparticle is considered as an example of infinitely reducible gauge algebras, and given explicitly its Sp(2) BRST invariant action. (author). 18 refs.

  12. Sp(2) covariant quantisation of general gauge theories

    International Nuclear Information System (INIS)

    Vazquez-Bello, J.L.

    1994-11-01

    The Sp(2) covariant quantization of gauge theories is studied. The geometrical interpretation of gauge theories in terms of quasi principal fibre bundles Q(M s , G s ) is reviewed. It is then described the Sp(2) algebra of ordinary Yang-Mills theory. A consistent formulation of covariant Lagrangian quantisation for general gauge theories based on Sp(2) BRST symmetry is established. The original N = 1, ten dimensional superparticle is considered as an example of infinitely reducible gauge algebras, and given explicitly its Sp(2) BRST invariant action. (author). 18 refs

  13. Enkapsulasi B. bassiana menggunakan maizena dan daya infeksinya terhadap larva Aedes aegypti, Anopheles sp., Culex sp.

    Directory of Open Access Journals (Sweden)

    Mutiara Widawati

    2015-12-01

    Full Text Available Abstract. Encapsulation formulae of mycoinsecticide have to be able to maintain fungus viability and pathogenicity. This mycoinsecticide was developed as an alternative way to control mosquito borne disease. The aim of this study was to encapsulate Beauveria bassiana as viable storage and have the capability to kill larvae of Aedes aegypti, Anopheles sp. and Culex sp. Mosquito larvae obtained from laboratory reared at health research laboratory; Loka Litbang P2B2 Ciamis. The treatments made in this study were the formu-lation of cornstarch and controls for comparison. This study showed potential formulation of cornstarch encapsulation as a biolarvacidal. Cornstarch formulations proven to be succeed in maintaining fungus viability, however, the pathogenicity of the microcapsule still not effective to kill Aedes, Culex and Anopheles larvae. Keywords: encapsulation, Beauveria bassiana, Ae.aegypti, Anopheles sp., Culex sp. Abstrak. Pembuatan formula bioinsektisida yang optimal sebagai salah satu alternatif untuk pengen-dalian nyamuk vektor perlu di kembangkan. Sediaan mikoinsektisida yang dibuat harus dapat memper-tahankan viabilitas jamur B. bassiana sehingga masih efektif pada saat penggunaannya. Salah satu cara yang digunakan untuk menjaga kestabilan sediaan mikoinsektisida yang berdampak langsung pada via-bilitas jamur adalah dengan menerapkan metode enkapsulasi. Penelitian ini bertujuan untuk meng-hasilkan sediaan mikokapsul dari Beauvaria bassiana melalui proses enkapsulasi menggunakan maizena yang memiliki kapabilitas tinggi sebagai penyimpan B. bassiana dan efektif dalam membunuh larva dan telur Aedes aegypti, Anopheles sp. dan Culex sp. Semua larva uji berasal dari insektarium laboratorium penelitian kesehatan Loka litbang P2B2 Ciamis. Pembuatan enkapsulasi dimulai dengan kultur dan pema-nenan B. bassiana, uji viabilitas, proses enkapsulasi serta uji larvasida di laboratorium. Uji dilakukan dengan satu perlakuan dan satu kontrol untuk

  14. Quality improvement on half-fin anchovy (Setipinna taty) fish sauce by Psychrobacter sp. SP-1 fermentation.

    Science.gov (United States)

    Zheng, Bin; Liu, Yu; He, Xiaoxia; Hu, Shiwei; Li, Shijie; Chen, Meiling; Jiang, Wei

    2017-10-01

    A method of improving fish sauce quality during fermentation was investigated. Psychrobacter sp. SP-1, a halophilic protease-producing bacterium, was isolated from fish sauce with flavor-enhancing properties and non-biogenic amine-producing activity. The performance of Psychrobacter sp. SP-1 in Setipinna taty fish sauce fermentation was investigated further. The inoculation of Psychrobacter sp. SP-1 did not significantly affect pH or NaCl concentration changes (P > 0.05), although it significantly increased total moderately halophilic microbial count, protease activity, total soluble nitrogen content and amino acid nitrogen content, and also promoted the umami taste and meaty aroma (P sauce quality by fermentation. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  15. Penggunaan Streptomyces sp. Sebagai Biokontrol Penyakit Layu Pada Tanaman Cabai Merah (Capsicum annuum L. yang Disebabkan Oleh Fusarium oxysporum f.sp. capsici

    Directory of Open Access Journals (Sweden)

    ANINDA OKTAVIA RAHARINI

    2014-01-01

    Full Text Available A research has been conducted to find out Streptomyces bacteria at Bukit Jimbaran, to inhibitionpotency of Streptomyces sp. to pathogenic fungi Fusarium oxysporum f.sp. capsici, and to find outantifungal activity of Streptomyces filtrate to F.oxysporum f.sp. capsici in chili (Capsicum annuumL. plants. Streptomyces sp. isolation was done by platting method with selective media YMA (ISP4.Identification of Streptomyces sp. used Bergey’s book entitled Manual Determinative Bacteriology.Test inhibition against F.oxysporum f.sp. capsici and in vivo test used by dying the roots of the chili(C.annuum L. plant with F.oxysporum f.sp. capsici and after 30 seconds the roots were dying withStreptomyces sp. culture, furthermore sterile soil on polybag watered by F.oxysporum f.sp. capsicispore and Streptomyces sp. culture at the same time. The result found five isolates Streptomyces sp.with different morphological. The antagonis test showed Streptomyces sp. 4 had ability (82% againstFusarium, Streptomyces sp.1 (72%, Streptomyces sp.2 (64%, Streptomyces sp.3 (76%, andStreptomyces sp. 5 (32%. All Streptomyces suppressed the growth of Fusarium on chili plants inglass house (p<0,05. Streptomyces sp.4 suppressed Fusarium wilt disease in chili from 80% in controlto 8%.

  16. UTILIZAÇÃO DE ADESIVO PVA EM COMPENSADOS DE Pinus sp. E Eucalyptus sp. / UTILIZATION OF PVA ADHESIVES IN Pinus sp. and Eucalyptus sp. PLYWOOD

    Directory of Open Access Journals (Sweden)

    C. I. De Campos

    2014-12-01

    Full Text Available Estudos sobre novos adesivos ou resinas para colagem de madeira e derivados estão sendo realizados com a intenção de diminuir o impacto ambiental sem alterar suas propriedades. Por este motivo, novas formulações de adesivos PVA (Acetato de Polivinila vêm sendo desenvolvidas. Este trabalho testou a utilização deste adesivo na produção de compensado de Eucalyptus sp. e Pinus sp., com tempo e temperatura de prensagem, respectivamente, de 10 minutos e90°C. Foram realizados os ensaios físicos de densidade, teor de umidade e absorção, e os ensaios mecânicos de flexão estática e de resistência ao cisalhamento na linha de cola, todos de acordo com as normas ABNT. Obtiveram-se bons resultados com relação à resistência ao cisalhamento na linha de cola, a qual foi superior para os adesivos PVA, se comparados com compensados produzidos com ureia-formaldeído e o fenol-formaldeído, enquanto que os resultados de MOE e MOR apresentaram-se inferiores em ambos os casos. 

  17. Microbial transformation of citral by Penicillium sp..

    Science.gov (United States)

    Esmaeili, Akbar; Tavassoli, Afsaneh

    2010-01-01

    Thymol is present in the essential oils from herbs and spices, such as thyme. It is produced by these plant species as a chemical defense against phytopathogenic microorganisms. Therefore, this compound has attracted great attention in food industry, i.e., it has been used as a natural preservative in foods such as cheese to prevent fungal growth. Previous studies concerning the biotransformation of nerol by Penicillium sp. and microbial transformation of citral by sporulated surface cultures method (SSCM) of Penicillium digitatum have been reported. The objective of this research was to study the pathway involved during biotransformation of citral by Penicillium sp. using two methods. The culture preparation was done using different microbial methods and incubation periods to obtain Penicillium for citral biotransformation. The biotransformation products were identified by gas chromatography (GC) and gas chromatography/mass spectroscopy (GC/MS). A comparison of the two methods showed that SSCM was more effective, its major products were thymol (21.5 %), geranial (18.6 %) and nerol (13.7 %). LM produced only one compound — thymol — with a low efficiency.

  18. Burkholderia cordobensis sp. nov., from agricultural soils.

    Science.gov (United States)

    Draghi, Walter O; Peeters, Charlotte; Cnockaert, Margo; Snauwaert, Cindy; Wall, Luis G; Zorreguieta, Angeles; Vandamme, Peter

    2014-06-01

    Two Gram-negative, rod-shaped bacteria were isolated from agricultural soils in Córdoba province in central Argentina. Their 16S rRNA gene sequences demonstrated that they belong to the genus Burkholderia, with Burkholderia zhejiangensis as most closely related formally named species; this relationship was confirmed through comparative gyrB sequence analysis. Whole-cell fatty acid analysis supported their assignment to the genus Burkholderia. Burkholderia sp. strain YI23, for which a whole-genome sequence is available, represents the same taxon, as demonstrated by its highly similar 16S rRNA (100% similarity) and gyrB (99.1-99.7%) gene sequences. The results of DNA-DNA hybridization experiments and physiological and biochemical characterization further substantiated the genotypic and phenotypic distinctiveness of the Argentinian soil isolates, for which the name Burkholderia cordobensis sp. nov. is proposed, with strain MMP81(T) ( = LMG 27620(T) = CCUG 64368(T)) as the type strain. © 2014 IUMS.

  19. The Effect of Acupuncture to SP6 on Skin Temperature Changes of SP6 and SP10: An Observation of “Deqi”

    Directory of Open Access Journals (Sweden)

    Jia-Min Yang

    2014-01-01

    Full Text Available Background. Deqi sensation is a complex but an important component for acupuncture effect. In this study, we tried to observe the relationship between Deqi and skin temperature changes and whether there was some relativity between Deqi and needle stimulations on cold congealing and dysmenorrhea rat model. Thirty-two female Sprague Dawley (SD rats were randomly divided into four groups (Saline Control Group, Model Group, Group A with strong stimulation, and Group B with small stimulation. Group A and Group B were performed with different stimulations. We found that, compared with saline control group, model group, and Group B, Group A showed that the skin temperature changes on right acupoint SP6 and SP10 increased significantly at 5 min–10 min interval. The skin temperature changes on left SP6 decreased at instant–5 min interval. The skin temperature changes on right SP10 decreased significantly at instant–5 min interval and 10 min–20 min interval. Thermogenic action along Spleen Meridian of Foot Greater Yin was manifested as simultaneous skin temperature increase on right SP6 and SP10 at 5 min–10 min interval after needling SP6, which was helpful to illustrate the relationship between the characteristic of Deqi and needle stimulations.

  20. Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains

    Directory of Open Access Journals (Sweden)

    Samiha Sioud

    2009-01-01

    Full Text Available Streptomyces sp. US 24 and Streptomyces sp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E. coli ET12567 (pUZ8002, as a conjugal donor, carrying the integrative plasmid pSET152. For the Streptomyces sp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp. TN 58, the integration was in single copy at the attB site. Plasmid pSET152 was inherited every time for all analysed Streptomyces sp. US 24 and Streptomyces sp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA from Streptomyces sp. SK was expressed in strain Streptomyces sp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of the Streptomyces sp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch.

  1. Effect of garlic solution to Bacillus sp. removal

    Science.gov (United States)

    Zainol, N.; Rahim, S. R.

    2018-04-01

    Biofilm is a microbial derived sessile community characterized by cells that are irreversibly attached to a substratum or interface to each other, embedded in a matrix of extracellular polymeric substances that they have produced. Bacillus sp. was used as biofilm model in this study. The purpose of this study is to determine the effect of Garlic solution in term of ratio of water and Garlic solution (W/G) and ratio of Garlic solution to Bacillus sp. (GS/B) on Bacillus sp removal. Garlic solution was used to remove Bacillus sp. In this study, Garlic solution was prepared by crushing the garlic and mixed it with water. the Garlic solution was added into Bacillus sp. mixture and mixed well. The mixture then was spread on nutrient agar. The Bacillus sp. weight on agar plate was measured by using dry weight measurement method. In this study, initially Garlic solution volume and Garlic solution concentration were studied using one factor at time (OFAT). Later two-level-factorial analysis was done to determine the most contributing factor in Bacillus sp. removal. Design Expert software (Version 7) was used to construct experimental table where all the factors were randomized. Bacilus sp removal was ranging between 42.13% to 99.6%. The analysis of the results showed that at W/G of 1:1, Bacillus sp. removal increased when more Garlic solution was added to Bacillus sp. Effect of Garlic solution to Bacillus sp. will be understood which in turn may be beneficial for the industrial purpose.

  2. Rhodotorula rosulata sp. nov., Rhodotorula silvestris sp. nov. and Rhodotorula straminea sp. nov., novel myo-inositol-assimilating yeast species in the Microbotryomycetes.

    Science.gov (United States)

    Golubev, Wladyslav I; Scorzetti, Gloria

    2010-10-01

    Three novel species are described as Rhodotorula rosulata sp. nov. (type strain VKM Y-2962(T) =CBS 10977(T)), Rhodotorula silvestris sp. nov. (type strain VKM Y-2971(T) =CBS 11420(T)) and Rhodotorula straminea sp. nov. (type strain VKM Y-2964(T) =CBS 10976(T)) based on the study of eight isolates from needle litter. The new species, phylogenetically located within the Microbotryomycetes, are related to glucuronate-assimilating species of the genus Rhodotorula. Sequencing of the D1/D2 domains of the LSU rDNA gene and the internal transcribed spacer (ITS) region, as well as physiological characterization, revealed their distinct taxonomic positions.

  3. Potential topical natural repellent against Ae. aegypti, Culex sp. and Anopheles sp. mosquitoes

    Directory of Open Access Journals (Sweden)

    Dewi Nur Hodijah

    2014-08-01

    Full Text Available AbstrakLatar belakang:Minyak atsiri daun sirih diketahui mempunyai daya proteksi. Dibuatkan losion berdasarkan pengantar sediaan farmasi yang ditambahkan minyak atsiri daun nilam. Sediaan losion dipilih agar dapat menempel lebih lama di permukaan kulit. Tujuan penelitian ini untuk membandingkan daya proteksi antara losion dengan penambahan minyak nilam dan losion tanpa penambahan minyak nilam dibandingkan daya proteksi dengan DEET. Metode: Penelitian ini merupakan penelitian eksperimental laboratorium. Semua nyamuk uji berasal dari insektarium laboratorium penelitian kesehatan Loka litbang P2B2 Ciamis. Konsentrasi minyak atsiri daun sirih dalam losion adalah 4%; konsentrasi minyak nilam sebagai zat pengikat adalah 0,4%. Formula yang digunakan yaitu formula dasar yang ada pada pengantar sediaan farmasi. Uji repelensi dilakukan dengan menggunakan metoda yang direkomendasikan oleh Komisi pestisida.Hasil: Dihasilkan formulasi losion yang stabil dan masih memenuhi standar formulasi sediaan. Berdasarkan hasil, diperoleh data bahwa DEET dan losion hasil modifikasi memiliki rata-rata daya proteksi di atas 90% selama 6 jam terhadap nyamuk Ae.aegypti dan Culex sp. Kesimpulan: Penambahan minyak nilam pada losion sirih dapat meningkatkan daya proteksi terhadap hinggapan nyamuk Ae. aegypti dan Culex sp. (Health Science Indones 2014;1:44-8Kata kunci:repelen alamiah, minyak atsiri, daun sirih, daun nilam, Ae. aegypti, Culex sp.AbstractBackground: Betel leaf essential oil lotion has been known to have insect repellent properties. A lotion was made based on a pharmaceutical formula from a monograph where patchouli leaf essential oil was added. A lotion preparation was intended to enhance adherence of the formula on the surface of the skin. The purpose of this study was to compare protection percentage of lotion with patchouli oil and without patchouli oil lotion compared to DEET.Methods: This study is an experimental laboratory-based research. All mosquitoes

  4. Kroyeria deetsi n.sp. (Kroyeriidae: Siphonostomatoida), a parasitic ...

    African Journals Online (AJOL)

    Kroyeria deetsi n.sp. (Kroyeriidae: Siphonostomatoida) is described from both sexes collected from the gills of spinner sharks, Carcharhinus brevipinna (Müller & Henle, 1839), captured in the Indian Ocean off the coast of South Africa. Kroyeria deetsi n.sp. can easily be distinguished from all of its congeners because the ...

  5. Enhancement of clavulanic acid production by Streptomyces sp MU ...

    African Journals Online (AJOL)

    Purpose: To enhance clavulanic acid production using UV-mutagenesis on Streptomyces sp. NRC77. Methods: UV-mutagenesis was used to study the effect of Streptomyces sp. NRC77 on CA production. Phenotypic and genotypic identification methods of the promising mutant strain were characterized. Optimization of the ...

  6. HANSENULA WICKERHAMII SP. N., A NEW YEAST FROM FINNISH SOIL

    Science.gov (United States)

    Capriotti, Augusto

    1961-01-01

    Capriotti, Augusto (l'Università di Perugia, Perugia, Italy). Hansenula wickerhamii sp. n., a new yeast from Finnish soil. J. Bacteriol. 82:259–360. 1961.—Hansenula wickerhamii sp. n. is described; it was isolated from a Finnish soil, and is named in honor of Lynferd J. Wickerham. Images PMID:13690638

  7. Truncated recombinant human SP-D attenuates emphysema and type II cell changes in SP-D deficient mice

    Directory of Open Access Journals (Sweden)

    Mühlfeld Christian

    2007-10-01

    Full Text Available Abstract Background Surfactant protein D (SP-D deficient mice develop emphysema-like pathology associated with focal accumulations of foamy alveolar macrophages, an excess of surfactant phospholipids in the alveolar space and both hypertrophy and hyperplasia of alveolar type II cells. These findings are associated with a chronic inflammatory state. Treatment of SP-D deficient mice with a truncated recombinant fragment of human SP-D (rfhSP-D has been shown to decrease the lipidosis and alveolar macrophage accumulation as well as production of proinflammatory chemokines. The aim of this study was to investigate if rfhSP-D treatment reduces the structural abnormalities in parenchymal architecture and type II cells characteristic of SP-D deficiency. Methods SP-D knock-out mice, aged 3 weeks, 6 weeks and 9 weeks were treated with rfhSP-D for 9, 6 and 3 weeks, respectively. All mice were sacrificed at age 12 weeks and compared to both PBS treated SP-D deficient and wild-type groups. Lung structure was quantified by design-based stereology at the light and electron microscopic level. Emphasis was put on quantification of emphysema, type II cell changes and intracellular surfactant. Data were analysed with two sided non-parametric Mann-Whitney U-test. Main Results After 3 weeks of treatment, alveolar number was higher and mean alveolar size was smaller compared to saline-treated SP-D knock-out controls. There was no significant difference concerning these indices of pulmonary emphysema within rfhSP-D treated groups. Type II cell number and size were smaller as a consequence of treatment. The total volume of lamellar bodies per type II cell and per lung was smaller after 6 weeks of treatment. Conclusion Treatment of SP-D deficient mice with rfhSP-D leads to a reduction in the degree of emphysema and a correction of type II cell hyperplasia and hypertrophy. This supports the concept that rfhSP-D might become a therapeutic option in diseases that are

  8. Caracterización bromatológica de seis especies forrajeras en el Valle del Cauto, Cuba

    Directory of Open Access Journals (Sweden)

    Danis M. Verdecia Acosta

    2014-01-01

    Full Text Available Se realizaron seis experimentos simultáneos para determinar la composición bromatológica de leguminosas forrajeras en las condiciones edafoclimáticas del Valle del Cauto, Cuba. Se empleó un diseño en bloques al azar con seis réplicas y los tratamientos fueron las edades de rebrote de 60, 120 y 180 días para los árboles y arbustos ( Leucaena leucocephala, Tithonia diversifolia, Gliricidia sepium y Eritrina varie - gata y de 30, 45, 60, 75 y 90 días para las leguminosas rastreras ( Neonotonia wightii y Te - ramnus labialis , en los periodos lluvioso y poco lluvioso. Se determinaron MS, PB, Ca, P, Mg, Si, FND, FAD, LAD, celulosa, hemicelulosa, contenido celular, ceniza, MO, DIVMS, DV, DISMS, DMO, DFND, DFAD y DPB. Se realizaron análisis de conglomerados para agrupar las especies con características simila - res. Durante el periodo lluvioso se encontraron seis grupos y en el poco lluvioso siete grupos; con los mejores resultados, de forma integral, para la Neonotonia wightii, Teramnus labialis, Gliricidia sepium y Tithonia diversifolia , en el periodo lluvioso; y en el poco lluvioso, para Tithonia diversifolia, Gliricidia sepium y Erythri - na variegata a edades tempranas. Se concluye que la edad presentó un marcado efecto en la composición bromatológica al disminuir la calidad en la medida que la madurez avanza.

  9. Solar drying of rose (Rosa sp.) petals

    Energy Technology Data Exchange (ETDEWEB)

    Balladin, D.A.; Headley, Oliver [University of the West Indies, Center for Resource Management and Environmental Studies, St. Michael (Barbados)

    1999-10-01

    The rose (Rosa sp.) petals can be dried after 2 days at about 30degC reaching an equilibrium moisture content after 16 h using the solar wire basket dryer. The initial moisture content (wet wt basis) and final moisture content (dry wt basis), determined by the Dean-Stark toluene were 65.7 and 25.2% respectively. The intensity of the rose red coloured pigment (pelargonidin) decreased by a factor of 2.5 after drying. The pelargonidin ethanoic extract as an acid-base indicator, has a K{sub 4} value of 1 x 10{sup -4} mol 1{sup -1} and pH of end point 4 and imbibed on filter paper and allowed to air dry for 5 min showed excellent properties as acid-based test tapes. (Author)

  10. Electron microscope study of Sarcocystis sp

    Science.gov (United States)

    Zeve, V.H.; Price, D.L.; Herman, C.M.

    1966-01-01

    Sarcocystis sp. obtained from wild populations of grackles, Quiscalus quiscula (Linn.), were examined to clarify the effect of the parasite on the host. Electron micrographs are presented to show areas of muscle destruction adjacent to the parasite which appear to be mechanically produced by the parasite. The microtubules within the villus-like projections of the cyst suggest that their possible function is absorptive and/or conductive with regard to the production of a toxin or the conveyance of nutritive material to the developing cells. The proposed function of submembranous filaments and their relation to the conoid is discussed. Similarities in the ultrastructure to Toxoplasma and other protozoa tend to negate the relegation of Sarcocystis to the fungi and further emphasize its protozoan nature.

  11. Polyhydroxybutyrate accumulation by a Serratia sp.

    Science.gov (United States)

    Lugg, Harriet; Sammons, Rachel L; Marquis, Peter M; Hewitt, Christopher J; Yong, Ping; Paterson-Beedle, Marion; Redwood, Mark D; Stamboulis, Artemis; Kashani, Mitra; Jenkins, Mike; Macaskie, Lynne E

    2008-03-01

    A strain of Serratia sp. showed intracellular electron-transparent inclusion bodies when incubated in the presence of citrate and glycerol 2-phosphate without nitrogen source following pre-growth under carbon-limitation in continuous culture. About 1.3 mmol citrate were consumed per 450 mg biomass, giving a calculated yield of maximally 55% of stored material per g of biomass dry wt. The inclusion bodies were stained with Sudan Black and Nile Red (NR), suggesting a lipid material, which was confirmed as polyhydroxybutyrate (PHB) by analysis of molecular fragments by GC and by FTIR spectroscopy of isolated bio-PHB in comparison with reference material. Multi-parameter flow cytometry in conjunction with NR fluorescence, and electron microscopy, showed that not all cells contained heavy PHB bodies, suggesting the potential for increasing the overall yield. The economic attractiveness is enhanced by the co-production of nanoscale hydroxyapatite (HA), a possible high-value precursor for bone replacement materials.

  12. In vitro rootting of Dioscoreas sp.

    Directory of Open Access Journals (Sweden)

    Irma Quintero

    2003-07-01

    Full Text Available The Universidad de Córdoba s Vegetal Tissue Culture Laboratory evaluated the effect of naphthalenacetic acid (NAA on in vitro rooting of three yam cultivars (Dioscorea sp. in 2000. The effect of four hormone levels (0, 0.3, 0.6 and 0.9 mg/1 was studied on three yam cultivars (Diamantes-22,003 and 005. A random experimental design was used employing 4x3 factorial arrangement and 20 repetitions; each experimental unit consisted of a glass receptacle containing the culture medium and the explant (one segment nodal. The variables considered were the number of roots and their thickness, culture medium oxidation and callus production. Findings showed that both the hormone and genotype had an effect on all those variables considered in the study and interaction was significant (P

  13. Predicting fuel performance for SP-100 conditions

    International Nuclear Information System (INIS)

    Baars, R.E.

    1985-01-01

    This paper reports on methods for analyzing fuel designs proposed for the thermionic and thermoelectric concepts for SP-100 application. The proposed fuel design for the thermionic concept consisted of fully-enriched oxide fuel clad in chemical vapor deposition (CVD) tungsten, which also served as the emitter for the thermionic fuel element (TFE). The fuel density was 95% of theoretical with the linear heat rate flattened radially by removing fuel from the center of the fuel pellet. The fuel inner diameter varied from approx.0.45 in. at the core center to zero at the edge of the core. The as-fabricated gap between fuel and emitter was 10 mils radial. The emitter thickness was 80 mils, and the outer diameter was 1.099 in. The LIFE-4 code was used for evaluation of this concept after extensive review of the code and development of a procedure that corrects certain deficiencies noted in analysis of several tests

  14. Aspergillus waksmanii sp. nov. and Aspergillus marvanovae sp. nov., two closely related species in section Fumigati

    DEFF Research Database (Denmark)

    Hubka, Vit; Peterson, Stephen W.; Frisvad, Jens Christian

    2013-01-01

    Two new and phylogenetically closely related species in Aspergillus section Fumigati are described and illustrated. Homothallic Aspergillus waksmanii sp. nov. was isolated from New Jersey soil (USA) and is represented by the ex-type isolate NRRL 179T (=CCF 4266T=Thom 4138.HS2T=IBT 31900T......). Aspergillus marvanovae sp. nov. was isolated from water with high boracic acid anions content in Dukovany nuclear power station (Czech Republic). The sexual stage of this species is unknown, but the MAT1-1 locus was successfully amplified suggesting that the species is probably heterothallic and teleomorphic...... but is represented by only the ex-type isolate CCM 8003T (=CCF 4037T=NRRL 62486T=IBT 31279T=IFM 60873T). Both species can be distinguished from all previously described species in section Fumigati based on morphology, maximum growth temperature, sequence data from five unlinked loci and unique secondary metabolites...

  15. Six New Polyacetylenic Alcohols from the Marine Sponges Petrosia sp. and Halichondria sp.

    Science.gov (United States)

    Gabriel, Adeyemi Francis; Li, Zhen; Kusuda, Ryouhei; Tanaka, Chiaki; Miyamoto, Tomofumi

    2015-01-01

    Six new polyacetylenic alcohols, termed strongylotriols A and B; pellynols J, K, and L; and isopellynol A, together with three known polyacetylenic alcohols, pellynols A, B, and C were isolated from the marine sponges Petrosia sp., and Halichondria sp. collected in Okinawa, Japan. Their planer structures were determined based on 2D-NMR and mass spectrometric analysis of the degraded products by RuCl3 oxidation. The absolute stereochemistry of isolates was examined by their Mosher's esters. The strongylotriols were found to be optically pure compounds, whereas the pellynols are diastereomeric mixtures at the C-6 position. Proliferation experiments using the HeLa and K562 cell lines suggested that the essential structural units for activity are the "hexa-2,4-diyn-1,6-diol" and "pent-1-en-4-yn-3-ol" on the termini.

  16. Kinerja probiotik Bacillus sp. pada pendederan benih ikan lele Clarias sp. yang diinfeksi Aeromonas hydrophila

    Directory of Open Access Journals (Sweden)

    , Sukenda

    2016-12-01

    Full Text Available ABSTRACT This experiment was conducted to assess performance of Bacillus sp. probiotic on catfish juvenile Clarias sp. infected by Aeromonas hydrophila. The probiotic content in the diets were 0% (K+ and K-, 1%, and 2% in duplicates. This experiment used randomized design with four treatments and two replications. Juveniles with average body weight of 3.22±0.15 g/fish were reared in the 1.5×2.8×0.5 m3 pond with density of 800 fish/pond. Fish were reared for 30 days and fed three times a day at rate 8% of  total body weight. At day 31, catfish were challenged by A. hydrophila 0.1 mL (106 cfu/mL. Post infection observation was carried out ten days with density 10 fish/aquaria. The result showed that fish fed diet containing 2% probiotic gave the best probiotic performance with survival rate of catfish 83.33% after challenged, spesific growth rate 5.40%, and 0,75 of feed conversion ratio. The results of the blood profile showed significantly better results in the treatment of probiotics compared to the positive control after challenge test A. hydrophila. Probiotic Bacillus sp. has given as much as 2% on feed provides better performance on catfish juvenile. Keywords: probiotic, Bacillus sp., A. hydrophila, catfish juvenille, growth  ABSTRAK Penelitian ini bertujuan untuk menguji kinerja probiotik Bacillus sp. dalam pakan pada pendederan benih ikan lele Clarias sp. yang diinfeksi bakteri Aeromonas hydrophila. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan yaitu kandungan probiotik dalam pakan perlakuan yaitu 0% (K+ dan K-, 1%,  dan 2%, masing-masing dengan dua ulangan. Ikan lele yang digunakan memiliki bobot rata-rata 3,22±0,15 g/ekor, dipelihara dalam kolam terpal berukuran 1,5×2,8×0,5 m3 dengan kepadatan 800 ekor/kolam. Ikan dipelihara selama 30 hari dengan frekuensi pemberian pakan tiga kali sehari sebanyak 8% dari bobot tubuh ikan. Hari ke-31 benih lele diinjeksi bakteri A. hydrophila dosis 0,1 m

  17. Descriptions of Deladenus albizicus n. sp. and D. processus n. sp. (Nematoda: Hexatylina) from Haryana, India.

    Science.gov (United States)

    Tomar, V V S; Somvanshi, Vishal S; Bajaj, Harish K

    2015-03-01

    Two different nematodes were isolated from the bark of Albizia lebbeck trees; one from insect infested and another from noninfested, healthy tree. Based on the biological, morphological, and molecular evidences, the nematodes are described as Deladenus albizicus n. sp. and D. processus n. sp. (Nematoda: Hexatylina). Deladenus albizicus n. sp., isolated from insect-infested tree, multiplied on the fungus Nigrospora oryzae. Myceliophagous females of this nematode reproduced by parthenogenesis and spermathecae were indistinct. Infective females, readily produced in the cultures, are dorsally curved. Only one type of males containing small-sized sperms in their genital tracts were produced in the culture. Myceliophagous females: L = 0.75 to 1.71 mm, a = 32.3 to 50.8, b = 9.3 to 11.2, b' = 5.2 to 7.3, c = 27.2 to 35.6, V = 91.0 to 93.3, c' = 2.0 to 2.9, stylet = 11 to 12 µm, excretory pore in the region of median pharyngeal bulb, 43 to 47 µm anterior to hemizonid. Deladenus processus n. sp., isolated from bark of healthy A. lebbeck tree, was cultured on Alternaria alternata. Myceliophagous females reproduced by amphimixis and their spermathecae contained rounded sperms. Infective females were never produced, even in old cultures. Myceliophagous females: L = 0.76 to 0.99 mm, a = 34 to 49, b = 13.3 to 17.7, b' = 3.8 to 5.8, c = 19.6 to 22.8, V = 92.2 to 93.5, c' = 2.7 to 3.5, stylet = 6 to 7 µm, excretory pore in the proximity of hemizonid, tail conoid, tapering from both sides to a long pointed central process. It is proposed to classify Deladenus species in three groups: durus, siricidicola, and laricis groups based on female and spermatogonia dimorphism, mode of reproduction, and insect parasitism.

  18. Neorickettsia risticii, Rickettsia sp. and Bartonella sp. in Tadarida brasiliensis bats from Buenos Aires, Argentina.

    Science.gov (United States)

    Cicuttin, Gabriel L; De Salvo, María N; La Rosa, Isabel; Dohmen, Federico E Gury

    2017-06-01

    Bats are potential reservoirs of many vector-borne bacterial pathogens. The aim of the present study was to detect species of Anaplasma, Ehrlichia, Neorickettsia, Rickettsia, Borrelia and Bartonella in Brazilian free-tailed bats (Tadarida brasiliensis, Molossidae) from Buenos Aires city, Argentina. Between 2012 and 2013, 61 T. brasiliensis from urban areas of Buenos Aires city were studied. The samples were molecularly screened by PCR and sequencing. Five bats (8.2%) were positive to Neorickettsia risticii, one (1.6%) was positive to Rickettsia sp. and three bats (4.9%) to Bartonella sp. For molecular characterization, the positive samples were subjected to amplification and sequencing of a fragment of p51 gene for N. risticii, a fragment of citrate synthase gene (gltA) for Rickettsia genus and a fragment of gltA for Bartonella genus. Phylogenetic tree was constructed using the maximum-likelihood method. Phylogenetic analysis of N. risticii detect in our study revealed that it relates to findings in the USA West Coast; Rickettsia sp. detected is phylogenetically within R. bellii group, which also includes many other Rickettsia endosymbionts of insects; and Bartonella sp. found is related to various Bartonella spp. described in Vespertilionidae bats, which are phylogenetically related to Molossidae. Our results are in accordance to previous findings, which demonstrate that insectivorous bats could be infected with vector-borne bacteria representing a potential risk to public health. Future research is necessary to clarify the circulation of these pathogens in bats from Buenos Aires. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Endosulfan induced alteration in bacterial protein profile and RNA yield of Klebsiella sp. M3, Achromobacter sp. M6, and Rhodococcus sp. M2.

    Science.gov (United States)

    Singh, Madhu; Singh, Dileep Kumar

    2014-01-30

    Three bacterial strains identified as Klebsiella sp. M3, Achromobacter sp. M6 and Rhodococcus sp. M2 were isolated by soil enrichment with endosulfan followed by shake flask enrichment technique. They were efficiently degrading endosulfan in the NSM (non sulfur medium) broth. Degradation of endosulfan was faster with the cell free extract of bacterial cells grown in the sulfur deficient medium (NSM) supplemented with endosulfan than that of nutrient rich medium (Luria Bertani). In the cell free extract of NSM supplemented with endosulfan as sole sulfur source, a unique band was visualized on SDS-PAGE but not with magnesium sulfate as the sole sulfur source in NSM and LB with endosulfan. Expression of a unique polypeptide band was speculated to be induced by endosulfan under sulfur starved condition. These unique polypeptide bands were identified as OmpK35 protein, sulfate binding protein and outer membrane porin protein, respectively, in Klebsiella sp. M3, Achromobacter sp. M6 and Rhodococcus sp. M2. Endosulfan showed dose dependent negative effect on total RNA yield of bacterial strains in nutrient rich medium. Absence of plasmid DNA indicated the presence of endosulfan metabolizing gene on genomic DNA. Copyright © 2013 Elsevier B.V. All rights reserved.

  20. From spent graphite to amorphous sp2+sp3 carbon-coated sp2 graphite for high-performance lithium ion batteries

    Science.gov (United States)

    Ma, Zhen; Zhuang, Yuchan; Deng, Yaoming; Song, Xiaona; Zuo, Xiaoxi; Xiao, Xin; Nan, Junmin

    2018-02-01

    Today, with the massive application of lithium ion batteries (LIBs) in the portable devices and electric vehicles, to supply the active materials with high-performances and then to recycle their wastes are two core issues for the development of LIBs. In this paper, the spent graphite (SG) in LIBs is used as raw materials to fabricate two comparative high-capacity graphite anode materials. Based on a microsurgery-like physical reconstruction, the reconstructed graphite (RG) with a sp2+sp3 carbon surface is prepared through a microwave exfoliation and subsequent spray drying process. In contrast, the neural-network-like amorphous sp2+sp3 carbon-coated graphite (AC@G) is synthesized using a self-reconfigurable chemical reaction strategy. Compared with SG and commercial graphite (CG), both RG and AC@G have enhanced specific capacities, from 311.2 mAh g-1 and 360.7 mAh g-1 to 409.7 mAh g-1 and 420.0 mAh g-1, at 0.1C after 100 cycles. In addition, they exhibit comparable cycling stability, rate capability, and voltage plateau with CG. Because the synthesis of RG and AC@G represents two typical physical and chemical methods for the recycling of SG, these results on the sp2+sp3 carbon layer coating bulk graphite also reveal an approach for the preparation of high-performance graphite anode materials derived from SG.

  1. Wnt-mediated down-regulation of Sp1 target genes by a transcriptional repressor Sp5

    Czech Academy of Sciences Publication Activity Database

    Fujimura, Naoko; Vacík, Tomáš; Machoň, Ondřej; Vlček, Čestmír; Scalabrin, S.; Speth, M.; Diep, D.; Krauss, S.; Kozmik, Zbyněk

    2007-01-01

    Roč. 282, č. 2 (2007), s. 1225-1237 ISSN 0021-9258 Institutional research plan: CEZ:AV0Z50520514 Keywords : Wnt -mediated signaling * Sp5 transcription factor * Sp1 target genes Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 5.581, year: 2007

  2. Structural Analysis of Planar sp3 and sp2 Films: Diamond-Like Carbon and Graphene Overlayers

    KAUST Repository

    Mansour, Ahmed

    2011-01-01

    pure sp2 as in graphene, carbon nanotubes and fullerenes, to pure sp3 as in diamond. Amorphous and diamond-like carbon consists of a mixture of both hybridizations. This variation in hybridization in carbon materials enables a wide spectrum

  3. Formulation of a minimal nutritional medium for enhanced lipid productivity in Chlorella sp. and Botryococcus sp. using response surface methodology.

    Science.gov (United States)

    Vishwakarma, Rashi; Dhar, Dolly Wattal; Pabbi, Sunil

    2018-03-01

    Chlorella sp. MCC 7 and Botryococcus sp. MCC 31 were investigated to enable large-scale biodiesel production from minimal constituents in the growth medium. Response surface methodology (RSM) was used to maximise the biomass productivity and lipid yield using only nitrogen (N), phosphorus (P) and potassium (K) as urea, single super phosphate and muriate of potash. The optimum values were 0.42 g/L nitrogen; 0.14 g/L phosphorus and 0.22 g/L potassium for Chlorella sp.; and 0.46 g/L; 0.14 g/L and 0.25 g/L for Botryococcus sp. Lipid yield of 42% for Chlorella sp. and 52% in Botryococcus sp. was observed. An enhancement in lipid yield by approximately 55% for Chlorella sp. and 73% for Botryococcus sp. was registered as compared to original nutrient medium. Fourier transform infrared (FTIR) analysis of extracted lipids revealed characteristic bands for triglycerides. This study provided utilisation of a practicable nutrient recipe in the form of N, P, K input for enhanced lipid yield from the selected microalgal strains.

  4. Elevated expression and potential roles of human Sp5, a member of Sp transcription factor family, in human cancers

    International Nuclear Information System (INIS)

    Chen Yongxin; Guo Yingqiu; Ge Xijin; Itoh, Hirotaka; Watanabe, Akira; Fujiwara, Takeshi; Kodama, Tatsuhiko; Aburatani, Hiroyuki

    2006-01-01

    In this report, we describe the expression and function of human Sp5, a member of the Sp family of zinc finger transcription factors. Like other family members, the Sp5 protein contains a Cys2His2 zinc finger DNA binding domain at the C-terminus. Our experiments employing Gal4-Sp5 fusion proteins reveal multiple transcriptional domains, including a N-terminal activity domain, an intrinsic repressive element, and a C-terminal synergistic domain. Elevated expression of Sp5 was noted in several human tumors including hepatocellular carcinoma, gastric cancer, and colon cancer. To study the effects of the Sp5 protein on growth properties of human cancer cells and facilitate the identification of its downstream genes, we combined an inducible gene expression system with microarray analysis to screen for its transcriptional targets. Transfer of Sp5 into MCF-7 cells that expressed no detectable endogenous Sp5 protein elicited significant growth promotion activity. Several of the constitutively deregulated genes have been associated with tumorigenesis (CDC25C, CEACAM6, TMPRSS2, XBP1, MYBL1, ABHD2, and CXCL12) and Wnt/β-Catenin signaling pathways (BAMBI, SIX1, IGFBP5, AES, and p21 WAF1 ). This information could be utilized for further mechanistic research and for devising optimized therapeutic strategies against human cancers

  5. The human luteinizing hormone receptor gene promoter: activation by Sp1 and Sp3 and inhibitory regulation.

    Science.gov (United States)

    Geng, Y; Tsai-Morris, C H; Zhang, Y; Dufau, M L

    1999-09-24

    To understand the transcriptional mechanism(s) of human LH receptor (LHR) gene expression, we have identified the dominant functional cis-elements that regulate the activity of the promoter domain (-1 to -176 bp from ATG). Mutagenesis demonstrated that the promoter activity was dependent on two Sp1 domains (-79 bp, -120 bp) in a transformed normal placental cell (PLC) and the choriocarcinoma JAR cell. Both elements interacted with endogenous Sp1 and Sp3 factors but not with Sp2 or Sp4. In Drosophila SL2 cells, the promoter was activated by either Sp1 or Sp3. An ERE half-site (EREhs) at -174 bp was inhibitory (by 100%), but was unresponsive to estradiol and did not bind the estrogen receptor or orphan receptors ERR1 and SF-1. The 5' upstream sequence (-177 to -2056 bp) inhibited promoter activity in PLC by 60%, but only minimally in JAR cells. Activation of the human LHR promoter through Sp1/3 factors is negatively regulated through EREhs and upstream sequences to exert control of gene expression. Copyright 1999 Academic Press.

  6. PENGGUNAAN EKSTRAK BIOTA LAUTAGLAOPHENIA SP. UNTUK MENGENDALIKANFUSARIUM OXYSPORUM F.SP.VANILLAE PENYEBAB BUSUK BATANG VANILI

    Directory of Open Access Journals (Sweden)

    I KETUT SUADA

    2012-11-01

    Full Text Available The Use of Aglaophenia sp. Marine Biota Extract to Control Fusarium oxysporum f. sp. vanillae, a Pathogen of Vanilla Stem Rot The use of marine biota for organic fungicide material has enormous potential to be developed. The organic fungicide was directed to substitute synthetic fungicide for vanilla cultivation. Application of synthetic fungicide degraded environmental quality, therefore its applications does not support sustainable agriculture. The objective of this research was to know the potence of Aglaopheniain suppressing the growth of F. oxysporum f. sp.vanillae, the pathogen of vanilla stem rot. The research was done in green house in order to find the best substance for formulation of the extract. The substances screened were water, tween-80, and detergents with two different concentrations. Subsequently, two best formulas were chosen to apply in endemic area in Tabanan. The green house and field trial research used Randomized Completely Design with three and five replications respectively. Results showed that tween was the best substance used for formulation and concentration of 0.2% was better than other concentrations. The extract diluted in tween-80 on the concentration of 0.2% showed the lowest rot on stem, longest shoot, and the most leaves on vanilla shoot. Because of its significant performance, the 0.1% extract ofAglaopheniain 0.1% tween-80 was proposed to be the best formula to suppress the vanilla stem rot disease.

  7. A sp2+sp3 hybridized carbon allotrope transformed from AB stacking graphyne and THD-graphene

    Directory of Open Access Journals (Sweden)

    Yu Zhang

    2018-01-01

    Full Text Available New carbon allotropes can be designed by combining sp, sp2 and sp3 three hybridization states. And the hybridization states or coordination numbers of carbon atoms can be changed by applying high pressure on carbon materials. In this study, a common high pressure phase (named as TBBC transformed from AB-stacking graphyne or THD-graphene is predicted. Its kinetic stability is examined using finite displacement method. We find that the sp2 and sp3 hybridized carbon atoms behave different vibration features at high frequency region. Both graphene-like and diamond-like vibration peaks occurs. Phase transition energy barriers from both graphyne and THD-graphene to TBBC are estimated. Electronic structure calculations show that the TBBC is an indirect semiconductor with a bandgap of 0.66 eV. The ideal tensile strength of TBBC is high in [0001] and [11¯00] directions, but is weak along [12¯10] direction.

  8. Kinetics of petroleum oil biodegradation by a consortium of three protozoan isolates (Aspidisca sp., Trachelophyllum sp. and Peranema sp.

    Directory of Open Access Journals (Sweden)

    L. Kachieng’a

    2017-09-01

    Full Text Available Petroleum oil is a complex mixture of substances, the majority of which are hydrocarbons; the latter represent an extremely important and heterogeneous group of compounds that find their way into water resources by anthropogenic or natural ways. The majority of toxic hydrocarbon components of petroleum are biodegradable, where bioremediation using microbial species has become an integral process for the restoration of oil-polluted areas. In this study, three bioremediation processes, namely natural attenuation, nutrient supplementation by adding glucose and biostimulation by adding Tween® 80, were carried out in various petroleum hydrocarbon concentrations in polluted water media using a consortium of three protozoan isolates (Aspidisca sp., Trachelophyllum sp. and Peranema sp.. A first-order kinetics model was fitted to the biodegradation data to evaluate the biodegradation rate and to determine the corresponding half-life time. First-order kinetics satisfactorily described the biodegradation of the petroleum-based contaminants under abiotic conditions. The results showed an increase in the percentage removal of petroleum oil at the lower petroleum concentrations and a gradual percentage decrease in removing petroleum oil residues occurred when there was an increase in the initial concentrations of the petroleum oil: 39%, 27%, 22%, 12%, 10% for various petroleum oil concentrations of 50, 100, 150, 200, 250 mg/L, respectively. A similar trend was also observed in the glucose-supplemented culture media where the reduction was 45% and 78% for petroleum concentrations of 250 mg/L and 50 mg/L, respectively. Biodegradation of between 33 and 90% was achieved at a Tween® 80 concentration of between 50 mg/L and 250 mg/L. The degradation rate constants for the natural attenuation process ranged between ≥0 to ≤0.50, ≥0 to ≤0.35, ≥0 to ≤0.25, ≥0 to ≤ 0.14 and ≥ 0 to ≤0.11 for petroleum oil concentrations varying from 50, 100, 150

  9. Cr(VI) reduction from contaminated soils by Aspergillus sp. N2 and Penicillium sp. N3 isolated from chromium deposits.

    Science.gov (United States)

    Fukuda, Tsubasa; Ishino, Yasuhiro; Ogawa, Akane; Tsutsumi, Kadzuyo; Morita, Hiroshi

    2008-10-01

    Aspergillus sp. N2 and Penicillium sp. N3 are chromate-resistant filamentous fungi that were isolated from Cr(VI) contaminated soil based on their ability to decrease hexavalent chromium levels in the growth medium. After 120 h of growth in a medium containing 50 ppm Cr(VI) at near neutral pH, Aspergillus sp. N2 reduced the Cr(VI) concentration by about 75%. Penicillium sp. N3 was able to reduce the Cr(VI) concentration by only 35%. However, Penicillium sp. N3 reduced the Cr(VI) concentration in the medium by 93% under acidic conditions. Interestingly, the presence of Cu(II) enhanced the Cr(VI) reducing ability of Aspergillus sp. N2 and Penicillium sp. N3 at near neutral pH. Aspergillus sp. N2 and Penicillium sp. N3 reduced the Cr(VI) concentration in the growth medium to a virtually undetectable level within 120 h. For both Aspergillus sp. N2 and Penicillium sp. N3, mycelial seed cultures were more efficient at Cr(VI) reduction than conidium seed cultures. The mechanisms of Cr(VI) reduction in Aspergillus sp. N2 and Penicillium sp. N3 were enzymatic reduction and sorption to mycelia. Enzymatic activity contributed significantly to Cr(VI) reduction. Aspergillus sp. N2 and Penicillium sp. N3 reduced the levels of Cr(VI) in polluted soil samples, suggesting that these strains might be useful for cleaning up chromium-contaminated sites.

  10. DAN IDENTIFIKASI PATOGEN POTENSIAL YANG MENGINFEKSI IKAN RAINBOW (Melanotaenia sp.

    Directory of Open Access Journals (Sweden)

    Lili Sholichah

    2014-03-01

    Full Text Available Pemeliharaan ikan rainbow (Melanotaenia sp. di Balai Penelitian dan Pengembangan Budidaya Ikan Hias selalu terjadi kematian secara bertahap mulai calon induk hingga proses pemijahan. Hal ini terjadi berulang kali sehingga ketersediaan induk Melanotaenia sp. sangat terancam. Ikan ini berasal dari Papua yang diperoleh mengandalkan penangkapan di alam. Tujuan dari penelitian ini adalah untuk menginventarisir dan mengidentifikasi berbagai patogen (parasit, jamur, bakteri potensial yang menginfeksi ikan rainbow yang dipelihara di dalam akuarium berukuran 50 cm x 50 cm x 50 cm dengan sistem aliran air stagnan. Tiga jenis rainbow yang dipelihara yaitu: rainbow Sungai Salawati, asal Sungai Sawiat, dan asal Danau Kurumoi. Setiap ikan masing-masing berjumlah 100 ekor dipelihara di akuarium dengan penambahan batu karang dan tanpa penambahan karang (kontrol ke dalam akuarium. Ikan diberi pakan sekenyangnya berupa jentik nyamuk dan cacing rambut beku setiap pagi dan sore hari. Sampling dilakukan secara random sebulan sekali dan secara unrandom setiap ada kejadian ikan sakit. Gejala klinis ikan yang sakit sebagai berikut: ikan berenang di permukaan dan menggosok-gosokkan badan di dinding akuarium, nafsu makan berkurang, gerakan berputar-putar, warna memudar menjadi putih, penekanan warna hitam pada sirip punggung dan perut meningkat, pendarahan pada perut, lendir berlebihan dan sangat berbau, serta sisik berdiri/terbuka. Diagnosa dan deteksi penyakit awal berupa pengamatan parasit baik ektoparasit maupun endoparasit, pengamatan dan isolasi jamur pada media selektif jamur, dan isolasi bakteri dilakukan untuk mengetahui jenis-jenis patogen yang menginfeksi ketiga jenis ikan rainbow. Selanjutnya dilakukan uji histologi dan analisa DNA beberapa patogen. Hasil pengamatan diperoleh patogen berupa parasit (Ichthyophthirius sp., Dactylogyrus sp., Gyrodactylus sp., dan Trichodina sp. dan bakteri (Aeromonas hydrophila, Acinetobacter sp

  11. Scalability of Parallel Spatial Direct Numerical Simulations on Intel Hypercube and IBM SP1 and SP2

    Science.gov (United States)

    Joslin, Ronald D.; Hanebutte, Ulf R.; Zubair, Mohammad

    1995-01-01

    The implementation and performance of a parallel spatial direct numerical simulation (PSDNS) approach on the Intel iPSC/860 hypercube and IBM SP1 and SP2 parallel computers is documented. Spatially evolving disturbances associated with the laminar-to-turbulent transition in boundary-layer flows are computed with the PSDNS code. The feasibility of using the PSDNS to perform transition studies on these computers is examined. The results indicate that PSDNS approach can effectively be parallelized on a distributed-memory parallel machine by remapping the distributed data structure during the course of the calculation. Scalability information is provided to estimate computational costs to match the actual costs relative to changes in the number of grid points. By increasing the number of processors, slower than linear speedups are achieved with optimized (machine-dependent library) routines. This slower than linear speedup results because the computational cost is dominated by FFT routine, which yields less than ideal speedups. By using appropriate compile options and optimized library routines on the SP1, the serial code achieves 52-56 M ops on a single node of the SP1 (45 percent of theoretical peak performance). The actual performance of the PSDNS code on the SP1 is evaluated with a "real world" simulation that consists of 1.7 million grid points. One time step of this simulation is calculated on eight nodes of the SP1 in the same time as required by a Cray Y/MP supercomputer. For the same simulation, 32-nodes of the SP1 and SP2 are required to reach the performance of a Cray C-90. A 32 node SP1 (SP2) configuration is 2.9 (4.6) times faster than a Cray Y/MP for this simulation, while the hypercube is roughly 2 times slower than the Y/MP for this application. KEY WORDS: Spatial direct numerical simulations; incompressible viscous flows; spectral methods; finite differences; parallel computing.

  12. Genetic polymorphism of horse serum protein 3 (SP3).

    Science.gov (United States)

    Juneja, R K; Sandberg, K; Kuryl, J; Gahne, B

    1989-01-01

    Two-dimensional agarose gel (pH 8.6)-horizontal polyacrylamide gel (pH 9.0) electrophoresis of horse serum samples, followed by general protein staining, revealed genetic polymorphism of an unidentified protein tentatively designated serum protein 3 (SP3). The SP3 fractions appeared distinctly when a 14% concentration of acrylamide was used in the separation gels. The 2-D mobilities of SP3 fractions were quite similar to that of albumin. Family data were consistent with the hypothesis that the observed SP3 phenotypes were controlled by four co-dominant, autosomal alleles (D, F, I, S). Evidence was provided that the F allele can be further divided into two alleles (F1 and F2); the mobilities of F1 and F2 variants were very similar. Each of the SP3 alleles gave rise to one fraction and each of the heterozygous types showed two fractions. More than 600 horses representing five different breeds (Swedish Trotter, North-Swedish Trotter, Thoroughbred, Arab and Polish Tarpan) were typed for SP3, and allele frequency estimates were calculated. SP3 was highly polymorphic in all breeds studied.

  13. A new species of Laricobius (Coleoptera: Derodontidae) from Japan with phylogeny and a key for native and introduced congeners in North America

    Science.gov (United States)

    Montgomery Michael E.; S. Shiyake; Nathan P. Havill

    2011-01-01

    Laricobius osakensis Montgomery and Shiyake sp. nov., collected from Adelges tsugae Annand on hemlock [Tsuga sieboldii Carr. and Tsuga diversifolia (Maxim.) Mast.] in Japan, is described and illustrated. The new species was collected from several localities on Honshu, Shikokou, and Kyushu...

  14. Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Ormeño-Orrillo Ernesto

    2012-12-01

    Full Text Available Abstract Background Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 are α-Proteobacteria that establish nitrogen-fixing symbioses with a range of legume hosts. These strains are broadly used in commercial inoculants for application to common bean (Phaseolus vulgaris in South America and Africa. Both strains display intrinsic resistance to several abiotic stressful conditions such as low soil pH and high temperatures, which are common in tropical environments, and to several antimicrobials, including pesticides. The genetic determinants of these interesting characteristics remain largely unknown. Results Genome sequencing revealed that CIAT 899 and PRF 81 share a highly-conserved symbiotic plasmid (pSym that is present also in Rhizobium leucaenae CFN 299, a rhizobium displaying a similar host range. This pSym seems to have arisen by a co-integration event between two replicons. Remarkably, three distinct nodA genes were found in the pSym, a characteristic that may contribute to the broad host range of these rhizobia. Genes for biosynthesis and modulation of plant-hormone levels were also identified in the pSym. Analysis of genes involved in stress response showed that CIAT 899 and PRF 81 are well equipped to cope with low pH, high temperatures and also with oxidative and osmotic stresses. Interestingly, the genomes of CIAT 899 and PRF 81 had large numbers of genes encoding drug-efflux systems, which may explain their high resistance to antimicrobials. Genome analysis also revealed a wide array of traits that may allow these strains to be successful rhizosphere colonizers, including surface polysaccharides, uptake transporters and catabolic enzymes for nutrients, diverse iron-acquisition systems, cell wall-degrading enzymes, type I and IV pili, and novel T1SS and T5SS secreted adhesins. Conclusions Availability of the complete genome sequences of CIAT 899 and PRF 81 may be exploited in further efforts to understand the interaction of tropical

  15. Reparation and Immunomodulating Properties of Bacillus sp. Metabolites from Permafrost.

    Science.gov (United States)

    Kalenova, L F; Melnikov, V P; Besedin, I M; Bazhin, A S; Gabdulin, M A; Kolyvanova, S S

    2017-09-01

    An ointment containing metabolites of Bacillus sp. microorganisms isolated from permafrost samples was applied onto the skin wound of BALB/c mice. Metabolites isolated during culturing of Bacillus sp. at 37°C produced a potent therapeutic effect and promoted wound epithelialization by 30% in comparison with the control (ointment base) and by 20% in comparison with Solcoseryl. Treatment with Bacillus sp. metabolites stimulated predominantly humoral immunity, reduced the time of wound contraction and the volume of scar tissue, and promoted complete hair recovery. These metabolites can be considered as modulators of the wound process with predominance of regeneration mechanisms.

  16. Post-neonatal drop in alveolar SP-A expression

    DEFF Research Database (Denmark)

    Stray-Pedersen, Arne; Vege, Ashild; Stray-Pedersen, Asbjorg

    2008-01-01

    BACKGROUND: Surfactant protein A (SP-A) is synthesized in the lung and is a part of the innate immune system. The aim of this study was to evaluate the expression of SP-A in lung tissue from fetuses, infants, children and adults with special regard to sudden infant death syndrome (SIDS). METHODS......: A total of 160 cases were studied; 19 fetuses and neonates, 59 SIDS and 49 explained infant deaths below 1 year of age, 19 toddlers and 14 adults. Immunohistochemical detection of SP-A using monoclonal antibodies was performed by microscopy of lung tissue specimens collected at autopsy. A scoring system...

  17. Efektivitas Ovitrap Bambu terhadap Jumlah Jentik Aedes sp yang Terperangkap

    Directory of Open Access Journals (Sweden)

    Wiwit Aditama

    2015-05-01

    places of 10 subdistricts. Data of trapped Aedes sp larvae numbers was collected four times repeatedly within one week time-lapse. Analysis was conducted using the mean number of larvae in ovitraps and ovitrap index. The number of Aedes sp larvae trapped was 1,265. The most effective ovitrap is piece of bamboo, mean = 123, p value = 0.006, HI = 10.01% (16.66 26.67%, CI = 36.8% (336.06 - 39.74%, BI = 29.97% (73.33 - 103.3%. Health authorities should promote bamboo ovitrap, especially to public as an effort to control Aedes sp.

  18. Späte Mutterschaft – (keine biografische Entscheidung

    Directory of Open Access Journals (Sweden)

    Martina Beham

    2004-11-01

    Full Text Available Der Übergang in die Mutterschaft findet zunehmend später statt. Über die Gründe liegen zahlreiche Vermutungen und einige theoretische Diskussionen, aber kaum empirische Befunde vor. Die Autorinnen möchten mit ihrer Studie einen Beitrag zum Abbau dieses Forschungsdefizits leisten. In ihrer empirisch-biografischen Untersuchung gehen Ingrid Herlyn und Dorothea Krüger der Frage nach, ob späte Erstmutterschaft ein neues biografisches Muster der Familiengründung darstellt oder ob Mutterschaft nach wie vor eine selbstverständlich anerkannte Norm ist, deren Realisierung im Lebenslauf lediglich zeitlich später stattfindet.

  19. Pengaruh Perbedaan Konsentrasi Ekstrak Sargassum sp. dan Lama Penyimpanan terhadap Oksidasi Lemak pada Fillet Ikan Patin (Pangasius sp.

    Directory of Open Access Journals (Sweden)

    Fatin Hidayati

    2017-05-01

    Full Text Available ABSTRAK Ikan patin merupakan ikan air tawar yang mengandung lemak dan protein tinggi sehingga apabila dilakukan penyimpanan rentan terjadi oksidasi yang mengakibatkan ketengikan. Sargassum sp. dengan kandungan fenol dan flavonoid mampu menghambat terjadinya oksidasi pada fillet ikan patin. Penelitian ini bertujuan untuk mengetahui pengaruh perbedaan ekstrak Sargassum sp. dan lama penyimpanan dalam menghambat terjadinya oksidasi pada fillet ikan patin. Materi yang digunakan dalam penelitian ini adalah ekstrak Sargassum sp. dan fillet ikan patin. Metode penelitian yang digunakan adalah experimental laboratories dengan menggunakan Rancangan Acak Lengkap (RAL faktorial dengan 2 faktor yaitu konsentrasi ekstrak Sargassum sp. (0%, 1%, 1,5% dan 2% dan lama penyimpanan (hari ke-0, hari ke-2, hari ke-4, dan hari ke-6. Hasil penelitian menunjukkan bahwa perbedaan penambahan konsentrasi ekstrak Sargassum sp. dan lama penyimpanan memberikan pengaruh nyata terhadap nilai PV, nilai TBA, kadar lemak, kadar protein, kadar air serta organoleptik (P < 0,05. Hasil penelitian tahap I didapatkan rendemen Sargassum sp. dengan pelarut etanol 96% sebesar 1,39%, kandungan fenol 1,813%, flavonoid 0,278% dan aktivitas antioksidan 99,1659 ppm (kuat. Hasil penelitian tahap II didapatkan nilai PV berkisar antara 2,03 - 19,82 meq/kg, nilai TBA 0,63 - 6,72 mg.mal/kg. Konsentrasi 1,5% merupakan konsentrasi terbaik ekstrak Sargassum sp. dalam menghambat oksidasi lemak pada fillet ikan patin selama penyimpanan. Kata kunci: Antioksidan, Ekstrak Sargassum sp., Lama Penyimpanan, Oksidasi lemak, Fillet Ikan patin ABSTRACT Catfish is a freshwater fish that contain high fat and protein so that if its stored it will susceptible to oxidation process which leads to rancidity. Sargassum sp. with its phenolic and flavonoid content are able to inhibit the oxidation process in catfish fillet. This research was aimed to know the effects of different concentrations of Sargassum sp. extracts and

  20. Mycorrhizal dependency of laurel (Ocotea sp.)

    International Nuclear Information System (INIS)

    Sierra-Escobar, Jorge A; Castro Restrepo, Dagoberto; Osorio Vega, Walter

    2009-01-01

    A greenhouse experiment was carried out to determine the mycorrhizal dependency of laurel (>Ocotea sp.). In order to do this, a completely randomized experimental design was used, with six treatments in a factorial array of 3 x 2 and five repetitions. The treatments involved a combination of three Phosphorus (P) levels in soil solution (0.002, 0.02 and 0.2 mg L-1) and two levels of mycorrhizal inoculation, either inoculated or non-inoculated with Glomus aggregatum Schenck and Smith. The leaf P content as a function of time was used as an output variable. Shoot dry matter, shoot P content, mycorrhizal colonization of roots, and mycorrhizal dependence were measured at harvest. The results indicated that the leaf P content increased significantly when using the mycorrhizal inoculation in laurel at P level 0.2 mg L -1, but not in the other P levels, on some of the sampling days. Shoot dry weight and total plant P content did not increase at all levels of soil available P. Mycorrhizal dependency of laurel reached 28%, which allows this species to be classified as moderately dependent on mycorrhiza.

  1. Production of biodiesel from Coelastrella sp. microalgae

    Science.gov (United States)

    Mansur, Dieni; Fitriady, Muhammad Arifuddin; Susilaningsih, Dwi; Simanungkalit, Sabar Pangihutan

    2017-11-01

    Microalgae have a wide area of usage and one of them it can be used for biodiesel production. In biodiesel production, lipids containing triglyceride or free fatty acid are converted into methyl ester through trans/esterification reactions. Lipids from microalgae can be extracted by acetone and dimethyl carbonate using homogenizer. Esterification of the lipids was investigated using various catalysts and source of methyl group. Activity of homogeneous catalyst such as HCl and H2SO4 and heterogeneous catalysts such as montmorillonit K-10 and ledgestone was investigated. Moreover, methanol and dimethyl carbonate as source of methyl group were also studied. Among of catalysts with methanol as source of methyl group, it was found that yield of crude biodiesel derived from Choelestrella Sp. microalgae was high over H2SO4 catalyst. On the other hand, over H2SO4 catalyst using dimethyl carbonate as source of methyl group, yield of crude biodiesel significant increase. However, FAME composition of crude biodiesel was high over HCl catalyst.

  2. Campylobacter iguaniorum sp. nov., isolated from reptiles.

    Science.gov (United States)

    Gilbert, Maarten J; Kik, Marja; Miller, William G; Duim, Birgitta; Wagenaar, Jaap A

    2015-03-01

    During sampling of reptiles for members of the class Epsilonproteobacteria, strains representing a member of the genus Campylobacter not belonging to any of the established taxa were isolated from lizards and chelonians. Initial amplified fragment length polymorphism, PCR and 16S rRNA sequence analysis showed that these strains were most closely related to Campylobacter fetus and Campylobacter hyointestinalis. A polyphasic study was undertaken to determine the taxonomic position of five strains. The strains were characterized by 16S rRNA and atpA sequence analysis, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and conventional phenotypic testing. Whole-genome sequences were determined for strains 1485E(T) and 2463D, and the average nucleotide and amino acid identities were determined for these strains. The strains formed a robust phylogenetic clade, divergent from all other species of the genus Campylobacter. In contrast to most currently known members of the genus Campylobacter, the strains showed growth at ambient temperatures, which might be an adaptation to their reptilian hosts. The results of this study clearly show that these strains isolated from reptiles represent a novel species within the genus Campylobacter, for which the name Campylobacter iguaniorum sp. nov. is proposed. The type strain is 1485E(T) ( = LMG 28143(T) = CCUG 66346(T)). © 2015 IUMS.

  3. Parapedobacter koreensis gen. nov., sp. nov.

    Science.gov (United States)

    Kim, Myung Kyum; Na, Ju-Ryun; Cho, Dong Ha; Soung, Nak-Kyun; Yang, Deok-Chun

    2007-06-01

    Strain Jip14(T), a Gram-negative, non-spore-forming, rod-shaped, non-motile bacterium, was isolated from dried rice straw and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain Jip14(T) belongs to the family Sphingobacteriaceae, and the highest degree of sequence similarity was determined to be to Pedobacter saltans DSM 12145(T) (88.5 %), Pedobacter africanus DSM 12126(T) (87.6 %), Pedobacter heparinus DSM 2366(T) (87.1 %) and Pedobacter caeni LMG 22862(T) (86.9 %). Chemotaxonomic data revealed that strain Jip14(T) possesses menaquinone MK-7 and the predominant fatty acids C(15 : 0) iso, C(16 : 0), C(16 : 0) 10-methyl, C(17 : 0) iso 3-OH and summed feature 3 (C(15 : 0) iso 2-OH/C(16 : 1)omega7c). The results of physiological and biochemical tests clearly demonstrated that strain Jip14(T) represents a distinct species. Based on these data, Jip14(T) should be classified within a novel genus and species, for which the name Parapedobacter koreensis gen. nov., sp. nov. is proposed. The type strain of Parapedobacter koreensis is Jip14(T) (=KCTC 12643(T)=LMG 23493(T)).

  4. Pedobacter insulae sp. nov., isolated from soil.

    Science.gov (United States)

    Yoon, Jung-Hoon; Kang, So-Jung; Oh, Hyun Woo; Oh, Tae-Kwang

    2007-09-01

    A Gram-negative, non-motile, rod-shaped bacterium, DS-139(T), was isolated from a soil sample collected from Dokdo, Korea, and subjected to a polyphasic taxonomic analysis. Strain DS-139(T) grew optimally at 25 degrees C and pH 6.5-7.5 in the presence of 0-0.5 % (w/v) NaCl. It contained MK-7 as the predominant menaquinone and iso-C(15 : 0), C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH and iso-C(17 : 0) 3-OH as the major fatty acids. The DNA G+C content was 39.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DS-39(T) belongs to the genus Pedobacter in the family Sphingobacteriaceae. The similarity values between the 16S rRNA gene sequence of strain DS-139(T) and those of the type strains of recognized Pedobacter species, except Pedobacter saltans, were in the range 93.9-96.7 %. The differential phenotypic properties, together with the phylogenetic distinctiveness, were sufficient to assign strain DS-139(T) to a species that is separate from recognized Pedobacter species. On the basis of the phenotypic and phylogenetic data, therefore, strain DS-139(T) represents a novel species of the genus Pedobacter, for which the name Pedobacter insulae sp. nov. is proposed. The type strain is DS-139(T) (=KCTC 12820(T) =DSM 18684(T)).

  5. Lactobacillus paralimentarius sp. nov., isolated from sourdough.

    Science.gov (United States)

    Cai, Y; Okada, H; Mori, H; Benno, Y; Nakase, T

    1999-10-01

    Six strains of lactic acid bacteria isolated from sourdough were characterized taxonomically. They were Gram-positive, catalase-negative, facultatively anaerobic rods that did not produce gas from glucose. Morphological and physiological data indicated that the strains belong to the genus Lactobacillus and they were similar to Lactobacillus alimentarius in phenotypic characteristics. These strains shared the same phenotypic characteristics and exhibited intragroup DNA homology values of over 89.8%, indicating that they comprised a single species. The G + C content of the DNA for the strains was 37.2-38.0 mol%. The 16S rRNA sequence of representative strain TB 1T was determined and aligned with that of other Lactobacillus species. This strain was placed in the genus Lactobacillus on the basis of phylogenetic analysis. L. alimentarius was the most closely related species in the phylogenetic tree and this species also showed the highest sequence homology value (96%) with strain TB 1T. DNA-DNA hybridization indicated that strain TB 1T did not belong to L. alimentarius. It is proposed that these strains are placed in the genus Lactobacillus as a new species, Lactobacillus paralimentarius sp. nov. The type strain of L. paralimentarius is TB 1T, which has been deposited in the Japan Collection of Microorganisms (JCM) as strain JCM 10415T.

  6. sp magnetism in clusters of gold thiolates

    International Nuclear Information System (INIS)

    Ayuela, A; Hernando, A; Echenique, P M; Crespo, P; García, M A

    2012-01-01

    Using first-principles calculations, we consider the bond between thiolate and small Au clusters, with particular emphasis on the resulting magnetic moment. The moment of pure gold clusters is 1 μ B for clusters with an odd number of Au atoms and zero for those with an even number. The addition of the thiolate, having an odd number of electrons itself, shifts the phase of the odd-even oscillations so that particles with an even number of Au atoms now have unit moment. Surprisingly, gold thiolate exhibits a dramatic and non-intuitive distribution of charge and spin moment. Our results show that the S-Au bond is such that sulfur does not get charge and an electron is transferred to the Au cluster. This extra electron is mainly sp in character and resides in an electronic shell below the Au surface. The calculations suggest that any thiolate-induced magnetism occurs in the gold nanoparticle and not the thiolate, and can be controlled by modifying the thiolate coverage. (paper)

  7. Preliminary SP-100/Stirling heat exchanger designs

    International Nuclear Information System (INIS)

    Schmitz, P.; Tower, L.; Blue, B.; Dunn, P.

    1994-01-01

    Analytic modeling of several heat exchanger concepts to couple the SP-100 nuclear reactor lithium loop and the Space Stirling Power Convertor (SSPC) was performed. Four 25 kWe SSPC's are used to produce the required 100 kW of electrical power. This design work focused on the interface between a single SSPC and the primary lithium loop. Manifolding to separate and collect the four channel flow was not modeled. This work modeled two separate types of heat exchanger interfaces (conductive coupling and radiative coupling) to explore their relative advantages and disadvantages. The minimum mass design of the conductively coupled concepts was 18 kg or 0.73 kg/kWe for a single 25 kWe convertor. The minimum mass radiatively coupled concept was 41 kg or 1.64 kg/kWe. The direct conduction heat exchanger provides a lighter weight system because of its ability to operate the Stirling convertor evaporator at higher heat fluxes than those attainable by the radiatively coupled systems. Additionally the conductively coupled concepts had relatively small volumes and provide potentially simpler assembly. Their disadvantages were the tight tolerances and material joining problems associated with this refractory to superalloy interface. The advantages of the radiatively coupled designs were the minimal material interface problems

  8. The Growth of Monoraphidium sp. and Scenedesmus sp. Cells in the Presence of Thorium

    Directory of Open Access Journals (Sweden)

    Juliana Cristina de Queiroz

    2012-01-01

    Full Text Available Toxicity of thorium by Monoraphidium sp. and Scenedesmus sp. was studied. Microalgal cultures were inoculated in ASM-1 medium in presence and absence of thorium. Its effect was monitored by direct counting on Fuchs-Rosenthal chamber and with software. The toxicity of thorium over the species was observed for concentrations over 50.0 mg/L. After 30 days, Monoraphidium cells decreased their concentration from 4.23×106 to 4.27×105 and 8.57×105 cells/mL, in the presence of 50.0 and 100.0 mg/L of thorium, respectively. Scenedesmus sp. cells were more resistant to thorium: for an initial cell concentration of 7.65×104 cells/mL it was observed a change to 5.25×105 and 5.12×105 cells/mL, in the presence of thorium at 50.0 and 100.0 mg/L, respectively. This is an indication that low concentrations of the radionuclide favored the growth, and that Scenedesmus cells are more resistant to thorium than Monoraphidium cells. The software used for comparison with direct count method proved to be useful for the improvement of accuracy of the results obtained, a decrease in the uncertainty and allowed recording of the data. The presence of thorium suggests that low concentrations have a positive effect on the growth, due to the presence of the nitrate, indicating its potential for ecotoxicological studies.

  9. The Growth of Monoraphidium sp. and Scenedesmus sp. Cells in the Presence of Thorium

    Science.gov (United States)

    de Queiroz, Juliana Cristina; Ferreira, Ana Cristina de Melo; da Costa, Antonio Carlos Augusto

    2012-01-01

    Toxicity of thorium by Monoraphidium sp. and Scenedesmus sp. was studied. Microalgal cultures were inoculated in ASM-1 medium in presence and absence of thorium. Its effect was monitored by direct counting on Fuchs-Rosenthal chamber and with software. The toxicity of thorium over the species was observed for concentrations over 50.0 mg/L. After 30 days, Monoraphidium cells decreased their concentration from 4.23 × 106 to 4.27 × 105 and 8.57 × 105 cells/mL, in the presence of 50.0 and 100.0 mg/L of thorium, respectively. Scenedesmus sp. cells were more resistant to thorium: for an initial cell concentration of 7.65 × 104 cells/mL it was observed a change to 5.25 × 105 and 5.12 × 105 cells/mL, in the presence of thorium at 50.0 and 100.0 mg/L, respectively. This is an indication that low concentrations of the radionuclide favored the growth, and that Scenedesmus cells are more resistant to thorium than Monoraphidium cells. The software used for comparison with direct count method proved to be useful for the improvement of accuracy of the results obtained, a decrease in the uncertainty and allowed recording of the data. The presence of thorium suggests that low concentrations have a positive effect on the growth, due to the presence of the nitrate, indicating its potential for ecotoxicological studies. PMID:22649297

  10. Down-regulation of human topoisomerase IIα expression correlates with relative amounts of specificity factors Sp1 and Sp3 bound at proximal and distal promoter regions

    Directory of Open Access Journals (Sweden)

    Isaacs Richard J

    2007-05-01

    Full Text Available Abstract Background Topoisomerase IIα has been shown to be down-regulated in doxorubicin-resistant cell lines. The specificity proteins Sp1 and Sp3 have been implicated in regulation of topoisomerase IIα transcription, although the mechanism by which they regulate expression is not fully understood. Sp1 has been shown to bind specifically to both proximal and distal GC elements of the human topoisomerase IIα promoter in vitro, while Sp3 binds only to the distal GC element unless additional flanking sequences are included. While Sp1 is thought to be an activator of human topoisomerase IIα, the functional significance of Sp3 binding is not known. Therefore, we sought to determine the functional relationship between Sp1 and Sp3 binding to the topoisomerase IIα promoter in vivo. We investigated endogenous levels of Sp1, Sp3 and topoisomerase IIα as well as binding of both Sp1 and Sp3 to the GC boxes of the topoisomerase IIα promoter in breast cancer cell lines in vivo after short term doxorubicin exposure. Results Functional effects of Sp1 and Sp3 were studied using transient cotransfection assays using a topoisomerase IIα promoter reporter construct. The in vivo interactions of Sp1 and Sp3 with the GC elements of the topoisomerase IIα promoter were studied in doxorubicin-treated breast cancer cell lines using chromatin immunoprecipitation assays. Relative amounts of endogenous proteins were measured using immunoblotting. In vivo DNA looping mediated by proteins bound at the GC1 and GC2 elements was studied using the chromatin conformation capture assay. Both Sp1 and Sp3 bound to the GC1 and GC2 regions. Sp1 and Sp3 were transcriptional activators and repressors respectively, with Sp3 repression being dominant over Sp1-mediated activation. The GC1 and GC2 elements are linked in vivo to form a loop, thus bringing distal regulatory elements and their cognate transcription factors into close proximity with the transcription start site

  11. Cloning and expression of an amylase gene from Bacillus sp ...

    African Journals Online (AJOL)

    SAM

    2014-08-06

    Aug 6, 2014 ... Bacillus sp. isolated from an agricultural field in West. Bengal, India ... plants, even though, the competition is incipient (Sen,. 2007), and therefore ..... proteins: Engineering mesophilic–like activity and stability in a cold adapted ...

  12. Itinerant ferromagnetism in fermionic systems with SP (2 N) symmetry

    Science.gov (United States)

    Yang, Wang; Wu, Congjun

    The Ginzburg-Landau free energy of systems with SP (2 N) symmetry describes a second order phase transition on the mean field level, since the Casimir invariants of the SP (2 N) group can be only of even order combinations of the generators of the SP (2 N) group. This is in contrast with systems having the SU (N) symmetry, where the allowance of cubic term generally makes the phase transition into first order. In this work, we consider the Hertz-Millis type itinerant ferromagnetism in an interacting fermionic system with SP (2 N) symmetry, where the ferromagnetic orders are enriched by the multi-component nature of the system. The quantum criticality is discussed near the second order phase transition point.

  13. Phenotyping and molecular characterization of Lysinibacillus sp. P ...

    African Journals Online (AJOL)

    ) branched with Lysinibacillus boronitolerans with 89% bootstrap support. Lysinibacillus sp. P-011 (~105 cfu/ml) played an important role on larval development of D. melanogaster under controlled environmental condition. Wild larvae when ...

  14. Achelia shepherdi n. sp. and other Pycnogonida from Australia

    NARCIS (Netherlands)

    Stock, Jan H.

    1973-01-01

    Records of 10 species of shallow water Pycnogonida from Western Australia, Victoria, Tasmania, and New South Wales, including Achelia shepherdi n. sp., Parapallene avida Stock, 1973 (♀ new to science), and Anoplodactylus pulcher Carpenter, 1907 (new to Australia).

  15. Benefit Analysis of SPC Panel SP-2 Projects

    National Research Council Canada - National Science Library

    Robinson, Rodney A

    1993-01-01

    This Task has investigated the benefits derived from the projects sponsored by SNAME Ship Production Committee Panel SP-2 on Outfitting and Production Aids during the 15 year period when this Panel...

  16. Economic analysis of variables affecting the plantain ( Musa sp ...

    African Journals Online (AJOL)

    Journal of Agriculture, Forestry and the Social Sciences ... The study examined variables affecting plantain (Musa sp) mono cropping system of production by ... land fragmentation, wrong application of fertilizer, high cost of fertilizers, lack of ...

  17. Linking surfactant protein SP-D and IL-13

    DEFF Research Database (Denmark)

    Qaseem, Asif S; Sonar, Sanchaita; Mahajan, Lakshna

    2012-01-01

    of allergen-IgE interaction, histamine release by sensitised mast cells, downregulation of specific IgE production, suppression of pulmonary and peripheral eosinophilia, inhibition of mechanisms that cause airway remodelling, and induction of apoptosis in sensitised eosinophils. SP-D can also shift helper T......Surfactant protein D (SP-D) is an innate immune molecule that plays a protective role against lung infection, allergy, asthma and inflammation. In vivo experiments with murine models have shown that SP-D can protect against allergic challenge via a range of mechanisms including inhibition...... cell polarisation following in vivo allergenic challenge, from pathogenic Th2 to a protective Th1 cytokine response. Interestingly, SP-D gene deficient (-/-) mice show an IL-13 over-expressing phenotype. IL-13 has been shown to be involved in the development of asthma. Transgenic mice over...

  18. Massarina armatispora sp. nov., a new intertidal ascomycete from mangroves

    Digital Repository Service at National Institute of Oceanography (India)

    Hyde, K.D.; Vrijmoed, L.L.P.; Chinnaraj, S.; Jones, E.B.G.

    Massarina armatispora sp. nov. is described from dead intertidal mangrove wood collected in India and Hong Kong. The new taxon is compared with other M. species, and its placement in the genus Massarina is discussed...

  19. Biomineralization of a calcifying ureolytic bacterium Microbacterium sp. GM-1

    Directory of Open Access Journals (Sweden)

    Guojing Xu

    2017-01-01

    Conclusions: The results of this research provide evidence that Microbacterium sp. GM-1 can biologically induce calcification and suggest that strain GM-1 may play a potential role in the synthesis of new biominerals and in bioremediation or biorecovery.

  20. Feather wastes digestion by new isolated strains Bacillus sp. in ...

    African Journals Online (AJOL)

    Feather wastes digestion by new isolated strains Bacillus sp. in Morocco. ... The most efficient isolated strain selected was compared with Bacillus subtilis ATCC 6633. Results showed ... African Journal of Biotechnology Vol.3(1) 2004: 67-70 ...

  1. Volvatellin, caulerpenyne-related product fromt he sacoglossan Volvatella sp.

    Digital Repository Service at National Institute of Oceanography (India)

    Fontana, A; Ciavatta, M.L; Mollo, E.; Naik, C.G.; Wahidullah, S.; DeSouza, L; Cimino, G.

    Volvatellin (4) is a highly unstable terpene isolated from the extracts of the Indian opisthobranch mollusk Volvatella sp. The structure and the relative stereochemistry of 4 were determined by NMR methods. The paper also describes a hypothetical...

  2. SP-100 multimegawatt scaleup to meet electric propulsion mission requirements

    International Nuclear Information System (INIS)

    Newkirk, D.W.; Salamah, S.A.; Stewart, S.L.; Pluta, P.R.

    1991-01-01

    The SP-100 space power nuclear reactor nuclear heat source technology, utilizing uranium nitride fuel clad in PWC-11 in a fast reactor with lithium coolant circulated by an electromagnetic pump, is shown in this paper to be directly extrapolatable to thermal power levels that meet NASA nuclear electric propulsion requirements using different power conversion techniques. The SP-100 nuclear technology can be applied for missions with NEP requirements as low as 10's of kWe to 10's of MWe

  3. Pigment Production from Immobilized Monascus sp. Utilizing Polymeric Resin Adsorption

    OpenAIRE

    Evans, Patrick J.; Wang, Henry Y.

    1984-01-01

    Pigment production by the fungus Monascus sp. was studied to determine why Monascus sp. provides more pigment in solid culture than in submerged culture. Adding a sterilized nonionic polymeric adsorbent resin directly to the growing submerged culture did not enhance the pigment production, thus indicating that pigment extraction is probably not a factor. Monascus cells immobilized in hydrogel were studied and exhibited decreased pigment production as a result of immobilization. This result is...

  4. Pengaruh PH dan Suhu terhadap Aktivitas Protease Penicillium SP.

    OpenAIRE

    Yusriah, Yusriah; Kuswytasari, Nengah Dwianita

    2013-01-01

    Tujuan penelitian ini adalah untuk mengetahui pengaruh pH dan suhu terhadap aktivitas protease pada Penicillium sp.3 T3f2. Selanjutnya, isolat Penicillium sp. di kultur dalam media produksi protease untuk menghasilkan protease. Suhu yang digunakan adalah 300 – 500C sedangkan pH-nya 4 – 8. Aktivitas protease ditentukan dan diukur dengan spektrofotometer pada panjang gelombang 275 nm, dengan kasein sebagai substrat. Berdasarkan uji ANOVA yang dilanjutkan dengan uji Duncan dengan taraf kepercaya...

  5. Environmental interactions and the SP-100 power system

    International Nuclear Information System (INIS)

    Ferguson, D.C.

    1993-01-01

    The Civil Space Technology Initiative High-Capacity-Power Environmental Interactions Program has made great progress in defining and evaluating the interactions of the SP-100 power system with its expected ambient environments. The NASCAP/LEO and POLAR computer codes demonstrated that local electric fields at the user interface module are high. Therefore, particular attention must be paid to geometries and materials in this region to prevent arcing at conductor-insulator junctions in low Earth orbit. NASCAP/LEO and EPSAT computer models revealed that SP-100 payloads float about 100 V negative of the LEO plasma. In addition, ground tests and modeling done for the Space Station Freedom Electrical Grounding Tiger Team found that dielectric coatings often break down at such voltages in a plasma. Thus, surface coatings for SP-100 payloads should be carefully selected. Sputtering may also be a concern for long-duration missions in LEO at these voltages. Much work has been done on a sputtering model to evaluate surface material loss rates on SP-100 payloads. In ground plasma chamber tests of cables and cable insulators at SP-100 voltages, parasitic power losses due to the plasma current collected from possible pinholes or coating defects were quantified and shown to be small. Modeling revealed that the power loss from currents to other surfaces is also small. The atomic oxygen durability of SP-100 materials and coatings continues to be investigated in ground tests. In the upcoming Evaluation of Oxygen Interaction with Materials (EOIM-3) Shuttle flight experiment, a host of SP-100 materials will be evaluated for atomic oxygen durability in LEO. Finally, an evaluation of the interactions of the SP-100 power system with lunar and planetary environments has started. At a workshop on chemical and electrical interactions on Mars recently held at the NASA Lewis Research Center, many of primary interactions were identified

  6. Genome sequencing and annotation of Serratia sp. strain TEL.

    Science.gov (United States)

    Lephoto, Tiisetso E; Gray, Vincent M

    2015-12-01

    We present the annotation of the draft genome sequence of Serratia sp. strain TEL (GenBank accession number KP711410). This organism was isolated from entomopathogenic nematode Oscheius sp. strain TEL (GenBank accession number KM492926) collected from grassland soil and has a genome size of 5,000,541 bp and 542 subsystems. The genome sequence can be accessed at DDBJ/EMBL/GenBank under the accession number LDEG00000000.

  7. Genome sequencing and annotation of Serratia sp. strain TEL

    Directory of Open Access Journals (Sweden)

    Tiisetso E. Lephoto

    2015-12-01

    Full Text Available We present the annotation of the draft genome sequence of Serratia sp. strain TEL (GenBank accession number KP711410. This organism was isolated from entomopathogenic nematode Oscheius sp. strain TEL (GenBank accession number KM492926 collected from grassland soil and has a genome size of 5,000,541 bp and 542 subsystems. The genome sequence can be accessed at DDBJ/EMBL/GenBank under the accession number LDEG00000000.

  8. Genome sequencing and annotation of Serratia sp. strain TEL

    OpenAIRE

    Lephoto, Tiisetso E.; Gray, Vincent M.

    2015-01-01

    We present the annotation of the draft genome sequence of Serratia sp. strain TEL (GenBank accession number KP711410). This organism was isolated from entomopathogenic nematode Oscheius sp. strain TEL (GenBank accession number KM492926) collected from grassland soil and has a genome size of 5,000,541 bp and 542 subsystems. The genome sequence can be accessed at DDBJ/EMBL/GenBank under the accession number LDEG00000000.

  9. Uranium and thorium uptake by live and dead cells of Pseudomonas Sp

    International Nuclear Information System (INIS)

    Siva Prasath, C.S.; Manikandan, N.; Prakash, S.

    2010-01-01

    This study presents uptake of uranium (U) and thorium (Th) by live and dead cells of Pseudomonas Sp. Increasing concentration of U and Tb showed decrease in absorption by Pseudomonas Sp. Dead cells of Pseudomonas Sp. exhibited same or more uptake of U and Th than living cells. Increasing temperature promotes uptake of U and Th by Pseudomonas Sp. (author)

  10. Evidence for cooperative mineralization of diuron by Arthrobacter sp. BS2 and Achromobacter sp. SP1 isolated from a mixed culture enriched from diuron exposed environments.

    Science.gov (United States)

    Devers-Lamrani, Marion; Pesce, Stéphane; Rouard, Nadine; Martin-Laurent, Fabrice

    2014-12-01

    Diuron was found to be mineralized in buffer strip soil (BS) and in the sediments (SED) of the Morcille river in the Beaujolais vineyard repeatedly treated with this herbicide. Enrichment cultures from BS and SED samples led to the isolation of three bacterial strains transforming diuron to 3,4-dichloroaniline (3,4-DCA) its aniline derivative. 16S rRNA sequencing revealed that they belonged to the genus Arthrobacter (99% of similarity to Arthrobacter globiformis strain K01-01) and were designated as Arthrobacter sp. BS1, BS2 and SED1. Diuron-degrading potential characterized by sequencing of the puhA gene, characterizing the diuron-degradaing potential, revealed 99% similarity to A. globiformis strain D47 puhA gene isolated a decade ago in the UK. These isolates were also able to use chlorotoluron for their growth. Although able to degrade linuron and monolinuron to related aniline derivatives they were not growing on them. Enrichment cultures led to the isolation of a strain from the sediments entirely degrading 3,4-DCA. 16S rRNA sequence analysis showed that it was affiliated to the genus Achromobacter (99% of similarity to Achromobacter sp. CH1) and was designated as Achromobacter sp. SP1. The dcaQ gene encoding enzyme responsible for the transformation of 3,4-DCA to chlorocatechol was found in SP1 with 99% similarity to that of Comamonas testosteroni WDL7. This isolate also used for its growth a range of anilines (3-chloro-4-methyl-aniline, 4-isopropylaniline, 4-chloroaniline, 3-chloroaniline, 4-bromoaniline). The mixed culture composed of BS2 and SP1 strains entirely mineralizes (14)C-diuron to (14)CO2. Diuron-mineralization observed in the enrichment culture could result from the metabolic cooperation between these two populations. Copyright © 2014. Published by Elsevier Ltd.

  11. Streptomyces sp. Sebagai Biofungisida Patogen Fusarium oxysporum (Schlecht. f.sp. lycopersici (Sacc. Snyd. et Hans. Penyebab Penyakit Layu Pada Tanaman Tomat (Solanum lycopersicum L.

    Directory of Open Access Journals (Sweden)

    NURI MANDAN SARI

    2014-01-01

    Full Text Available A research was conducted to isolate Streptomyces sp. of soil Udayana University campus in theBukit-Jimbaran, to obtain the most effective Streptomyces sp. which is effective in inhibit the growth ofFusarium oxysporum f.sp. lycopersici, and to test response of tomato plants with Streptomyces sp.culture against Fusarium wilt desease. Implementation phases of the research consisted of isolation andidentification of Streptomyces sp, test the inhibition against F. oxysporum f.sp. lycopersici, and in vivotest used by dyeing the roots of the tomato plant (Solanum lycopersicum with Fusarium spores andafter 30 seconds the roots were dyeing Streptomyces culture. Furthermore, sterile soil in polybagwatered by Fusarium spores and Streptomyces culture at the same time. Based on morphologicalcharacteristic it found five isolates of Streptomyces sp.. The antagonist test showed Streptomyces sp.1 had ability (75% against Fusarium, Streptomyces sp 2 (68,3%, Streptomyces sp. 3 (71,6%,Streptomyces sp. 4 (63,3%, and Streptomyces sp. 5 (21,6%. All Streptomyces suppressed thegrowth of Fusarium on tomato plants in glass house (p<0,05. Streptomyces sp.3 suppressed Fusariumwilt disease in tomato from 88% in control to 20%.

  12. Characterization of a bioflocculant produced by a consortium of Halomonas sp. Okoh and Micrococcus sp. Leo.

    Science.gov (United States)

    Okaiyeto, Kunle; Nwodo, Uchechukwu U; Mabinya, Leonard V; Okoh, Anthony I

    2013-10-16

    The physicochemical and flocculating properties of a bioflocculant produced by a bacterial consortium composed of Halomonas sp. Okoh and Micrococcus sp. Leo were investigated. The purified bioflocculant was cation and pH dependent, and optimally flocculated kaolin clay suspension at a dosage of 0.1 mg/mL. The flocculating activity of the bioflocculant was stimulated in the presence of Ca2+, Mn2+, Al3+ and had a wide pH range of 2-10, with the highest flocculating activity of 86% at pH 8. The bioflocculant was thermostable and retained more than 70% of its flocculating activity after being heated at 80 °C for 30 min. Thermogravimetric analyses revealed a partial thermal decomposition of the biofloculant at 400 °C. The infrared spectrum showed the presence of hydroxyl, carboxyl and amino moieties as functional groups. The bioflocculant produced by the bacterial consortium appears to hold promising alternative to inorganic and synthetic organic flocculants that are widely used in wastewater treatment.

  13. Identification and production of bioflocculants by Enterobacter sp. and Bacillus sp. and their characterization studies.

    Science.gov (United States)

    Muthulakshmi, L; Nellaiah, H; Kathiresan, T; Rajini, N; Christopher, Fenila

    2017-05-28

    In this work, two bioflocculants, namely, EB-EPS and B1-EPS, were derived from Enterobacter sp. and Bacillus sp., respectively, and analyzed with regard to their production and characterization. About 0.9 and 0.16 g of purified EB and B1 were obtained from I L of fermentation broth. Chemical analysis showed the contents of purified EB and B1 mainly as 88.7 and 92.8% (w/w) of carbohydrate, and 11.3 and 21.8% (w/w) protein, respectively. Fourier-transform infrared spectrometry analysis revealed the presence of hydroxyl, amide, and carboxyl groups in the identified bioflocculant. Thermogravimetric analysis (TGA) results exhibited enhanced thermal stability with a minimum mass loss of 50% while 25% were found to have occurred at higher temperatures (>400°C) for microbe-derived compounds EB and B1 leading to the possibility of using these compounds as fillers or for fabricating composite films for high-temperature applications. Further, the compounds from both the bacteria exhibited good antibacterial characteristics against pathogenic Escherichia coli. Degradability study of bioflocculant-embedded composite films shows the possibility of attaining eco-friendly bioremediation. Accordingly, experimental results revealed the suitability of developed composite films as a suitable alternative for food packaging and biomedical applications.

  14. Potencial de antagonismo de isolados de Trichoderma sp. contra o isolados de Fusarium sp., in vitro

    Directory of Open Access Journals (Sweden)

    Charles Anthony Hoffmann

    2015-06-01

    Full Text Available Resumo – O uso discriminado de agrotóxicos acarreta em uma grande prejuízo a saúdes das pessoas e do meio ambiente. O uso de novos métodos de combate a pragas se faz necessário para o equilíbrio da cadeia produtiva. A utilização de microrganismos no combate a pragas se faz como uma boa alternativa. O trabalho objetivou-se em avaliar o potencial de antagonismo de isolados de Trichoderma sp. contra o patógeno Fusarium sp. in vitro. Para isso foi utilizado os métodos de confronto direto, pareamento de culturas e de metabólitos voláteis. Os experimentos foram realizados na incubadora de empresas da Universidade Federal do Tocantins – UFT Campus de Gurupi, seguiram com três repetições. Foram testados 15 isolados do antagonista em confronto direto com o patógeno e os mesmos isolados e patógeno foram testados em metabólitos voláteis ambos utilizando o meio de cultura BDA. Todos os isolados apresentaram antagonismo in vitro contra o patógeno.

  15. Listeria booriae sp. nov. and Listeria newyorkensis sp. nov., from food processing environments in the USA.

    Science.gov (United States)

    Weller, Daniel; Andrus, Alexis; Wiedmann, Martin; den Bakker, Henk C

    2015-01-01

    Sampling of seafood and dairy processing facilities in the north-eastern USA produced 18 isolates of Listeria spp. that could not be identified at the species-level using traditional phenotypic and genotypic identification methods. Results of phenotypic and genotypic analyses suggested that the isolates represent two novel species with an average nucleotide blast identity of less than 92% with previously described species of the genus Listeria. Phylogenetic analyses based on whole genome sequences, 16S rRNA gene and sigB gene sequences confirmed that the isolates represented by type strain FSL M6-0635(T) and FSL A5-0209 cluster phylogenetically with Listeria cornellensis. Phylogenetic analyses also showed that the isolates represented by type strain FSL A5-0281(T) cluster phylogenetically with Listeria riparia. The name Listeria booriae sp. nov. is proposed for the species represented by type strain FSL A5-0281(T) ( =DSM 28860(T) =LMG 28311(T)), and the name Listeria newyorkensis sp. nov. is proposed for the species represented by type strain FSL M6-0635(T) ( =DSM 28861(T) =LMG 28310(T)). Phenotypic and genotypic analyses suggest that neither species is pathogenic. © 2015 IUMS.

  16. Glycomyces scopariae sp. nov. and Glycomyces mayteni sp. nov., isolated from medicinal plants in China.

    Science.gov (United States)

    Qin, Sheng; Chen, Hua-Hong; Klenk, Hans-Peter; Zhao, Guo-Zhen; Li, Jie; Xu, Li-Hua; Li, Wen-Jun

    2009-05-01

    Two actinomycete strains, designated YIM 56256(T) and YIM 61331(T), were isolated from the roots of Scoparia dulcis and Maytenus austroyunnanensis, two Chinese medicinal plants, and their taxonomic status was established based on a polyphasic investigation. The organisms were found to have chemical and morphological markers typical of members of the genus Glycomyces. 16S rRNA gene sequence analysis showed that they were closely related to each other and to Glycomyces sambucus E71(T). A battery of physiological characteristics and levels of DNA-DNA relatedness indicated that strains YIM 56256(T) and YIM 61331(T) represent two novel species, clearly different from the related known Glycomyces species. On the basis of the data presented, it is evident that each of these strains represents a novel species of the genus Glycomyces, for which the names Glycomyces scopariae sp. nov. (type strain YIM 56256(T) =KCTC 19158(T) =DSM 44968(T)) and Glycomyces mayteni sp. nov. (type strain YIM 61331(T) =KCTC 19527(T) =CCTCC AA 208004(T)) are proposed.

  17. Methylobacterium pseudosasicola sp. nov. and Methylobacterium phyllostachyos sp. nov., isolated from bamboo leaf surfaces.

    Science.gov (United States)

    Madhaiyan, Munusamy; Poonguzhali, Selvaraj

    2014-07-01

    Two strains of Gram-negative, methylotrophic bacteria, isolated because of their abilities to promote plant growth, were subjected to a polyphasic taxonomic study. The isolates were strictly aerobic, motile, pink-pigmented, facultatively methylotrophic, non-spore-forming rods. The chemotaxonomic characteristics of the isolates included the presence of C18 : 1ω7c as the major cellular fatty acid. The DNA G+C contents of strains BL36(T) and BL47(T) were 69.4 and 69.8 mol%, respectively. 16S rRNA gene sequence analysis of strains BL36(T) and BL47(T) placed them under the genus Methylobacterium, with the pairwise sequence similarity between them and the type strains of closely related species ranging from 97.2 to 99.0%. On the basis of their phenotypic and phylogenetic distinctiveness and the results of DNA-DNA hybridization analysis, the isolates represent two novel species within the genus Methylobacterium, for which the names Methylobacterium pseudosasicola sp. nov. (type strain BL36(T) = NBRC 105203(T) = ICMP 17621(T)) and Methylobacterium phyllostachyos sp. nov. (type strain BL47(T) = NBRC 105206(T) = ICMP 17619(T)) are proposed. © 2014 IUMS.

  18. Watery rot of pseudo-stem (Dickeya sp. management in banana (Musa sp. under greenhouse conditions

    Directory of Open Access Journals (Sweden)

    Joaquín Guillermo Ramírez

    2014-12-01

    Full Text Available This crop has serious constraints with diseases, including those caused by bacteria, such as Dickeya sp. This research aimed to evaluate the effect of 4 resistance inductors and 3 doses in Chlorine Dioxide in handling watery rot of pseudo-stem (Dickeya sp. in banana. Resistance inducers and their doses were Potassium Phosphite: 1.5 cm 3 .l -1 ; 3-Aminobutanoic Acid: 1.0 g.l -1 ; Methyl Jasmonate: 0.2 g.l -1 ; S-Methyl-Acibenzolar: 0.3 ml.l -1 , all by foliar application, while Chlorine Dioxide was injected into the pseudo-stem, in doses of 10, 20 and 30 mg.l -1 . The evaluated variables were: development of the disease, total biomass and quantification of the bacterium in the inoculated pseudo-stems. Applications of Chlorine Dioxide achieved a reduction of disease by 65.4, 91.99 and 61.5%, in addition to an inhibition of 100% of the pathogen, using 30 and 50 mg.l -1 doses. Meanwhile, the use of resistance inductors reduced up to 60.6% of the disease, but this effect failed to improve plant growth.

  19. Streptococcus loxodontisalivarius sp. nov. and Streptococcus saliviloxodontae sp. nov., isolated from oral cavities of elephants.

    Science.gov (United States)

    Saito, Masanori; Shinozaki-Kuwahara, Noriko; Hirasawa, Masatomo; Takada, Kazuko

    2014-09-01

    Four Gram-stain-positive, catalase-negative, coccoid-shaped organisms were isolated from elephant oral cavities. The isolates were tentatively identified as streptococcal species based on the results of biochemical tests. Comparative 16S rRNA gene sequencing studies confirmed the organisms to be members of the genus Streptococcus. Two isolates (NUM 6304(T) and NUM 6312) were related most closely to Streptococcus salivarius with 96.8 % and 93.1 % similarity based on the 16S rRNA gene and the RNA polymerase β subunit encoding gene (rpoB), respectively, and to Streptococcus vestibularis with 83.7 % similarity based on the 60 kDa heat-shock protein gene (groEL). The other two isolates (NUM 6306(T) and NUM 6318) were related most closely to S. vestibularis with 97.0 % and 82.9 % similarity based on the 16S rRNA and groEL genes, respectively, and to S. salivarius with 93.5 % similarity based on the rpoB gene. Based on phylogenetic and phenotypic evidence, these isolates are suggested to represent novel species of the genus Streptococcus, for which the names Streptococcus loxodontisalivarius sp. nov. (type strain NUM 6304(T) = JCM 19287(T) = DSM 27382(T)) and Streptococcus saliviloxodontae sp. nov. (type strain NUM 6306(T) = JCM 19288(T) = DSM 27513(T)) are proposed. © 2014 IUMS.

  20. Streptococcus moroccensis sp. nov. and Streptococcus rifensis sp. nov., isolated from raw camel milk.

    Science.gov (United States)

    Kadri, Zaina; Amar, Mohamed; Ouadghiri, Mouna; Cnockaert, Margo; Aerts, Maarten; El Farricha, Omar; Vandamme, Peter

    2014-07-01

    Two catalase- and oxidase-negative Streptococcus-like strains, LMG 27682(T) and LMG 27684(T), were isolated from raw camel milk in Morocco. Comparative 16S rRNA gene sequencing assigned these bacteria to the genus Streptococcus with Streptococcus rupicaprae 2777-2-07(T) as their closest phylogenetic neighbour (95.9% and 95.7% similarity, respectively). 16S rRNA gene sequence similarity between the two strains was 96.7%. Although strains LMG 27682(T) and LMG 27684(T) shared a DNA-DNA hybridization value that corresponded to the threshold level for species delineation (68%), the two strains could be distinguished by multiple biochemical tests, sequence analysis of the phenylalanyl-tRNA synthase (pheS), RNA polymerase (rpoA) and ATP synthase (atpA) genes and by their MALDI-TOF MS profiles. On the basis of these considerable phenotypic and genotypic differences, we propose to classify both strains as novel species of the genus Streptococcus, for which the names Streptococcus moroccensis sp. nov. (type strain, LMG 27682(T)  = CCMM B831(T)) and Streptococcus rifensis sp. nov. (type strain, LMG 27684(T)  = CCMM B833(T)) are proposed. © 2014 IUMS.

  1. Growth kinetics and biodeterioration of polypropylene microplastics by Bacillus sp. and Rhodococcus sp. isolated from mangrove sediment.

    Science.gov (United States)

    Auta, H S; Emenike, C U; Jayanthi, B; Fauziah, S H

    2018-02-01

    Interest in the biodegradation of microplastics is due to their ubiquitous distribution, availability, high persistence in the environment and deleterious impact on marine biota. The present study evaluates the growth response and mechanism of polypropylene (PP) degradation by Bacillus sp. strain 27 and Rhodococcus sp. strain 36 isolated from mangrove sediments upon exposure to PP microplastics. Both bacteria strains were able to utilise PP microplastic for growth as confirmed by the reduction of the polymer mass. The weight loss was 6.4% by Rhodococcus sp. strain 36 and 4.0% by Bacillus sp. strain 27 after 40days of incubation. PP biodegradation was further confirmed using Fourier-transform infrared spectroscopy and scanning electron microscopy analyses, which revealed structural and morphological changes in the PP microplastics with microbial treatment. These analyses showed that the isolates can colonise, modify and utilise PP microplastics as carbon source. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Enhanced photocatalytic activity induced by sp 3 to sp 2 transition of carbon dopants in BiOCl crystals

    KAUST Repository

    Sun, Jianguo

    2017-09-19

    The insufficient light absorption and low quantum efficiency limit the photocatalytic performance of wide bandgap semiconductors. Here, we report a facile strategy to engineer the surface disordered defects of BiOCl nanosheets via carbon doping. The surface defects boost the light absorption and also the quantum yields, as the doped carbon atoms exhibit a transition from sp3 to sp2 hybridization at elevated temperature, corresponding to a change of assembly state from 3D cluster to 2D graphite-like structure. This transition results in an effective charge separation and thus one order of enhancement in photocatalytic activity toward phenol degradation under visible light. The current study opens an avenue to introduce sp3 to sp2 transition of carbon dopants for simultaneous increment of light absorption and quantum efficiency for application in photocatalysis and energy conversion.

  3. Rhopalophthalmus constrictus sp. nov. and Australomysis reubeni sp. nov. (Crustacea-Mysidacea) from south-west Australian waters

    Digital Repository Service at National Institute of Oceanography (India)

    Panampunnayil, S.U.

    Two new species of mysids are described, Rhopalophthalmus constrictus sp. nov. is in having a well-produced triangular rostral plate, pleural plates on the anterior five abdominal somites and prominent constriction at the distal end of the telson...

  4. Gordonia humi sp. nov., isolated from soil.

    Science.gov (United States)

    Kämpfer, P; Young, Chiu-Chung; Chu, Jiunn-Nan; Frischmann, A; Busse, H-J; Arun, A B; Shen, Fo-Ting; Rekha, P D

    2011-01-01

    A Gram-stain-positive, non-endospore-forming actinobacterium (CC-12301(T)) was isolated from soil attached to a spawn used in the laboratory to grow the edible mushroom Agaricus brasiliensis. Based on 16S rRNA gene sequence similarities, strain CC-12301(T) was shown to belong to the genus Gordonia and was most closely related to the type strains of Gordonia hydrophobica (97.6 % similarity), Gordonia terrae (97.5 %), Gordonia amarae (97.5 %) and Gordonia malaquae (97.4 %). The quinone system was determined to consist predominantly of menaquinone MK-9(H(2)), minor amounts of MK-8(H(2)) and MK-7(H(2)). The polar lipid profile consisted of the major compounds diphosphatidylglycerol and phosphatidylethanolamine, moderate amounts of two phosphatidylinositol mannosides and phosphatidylinositol and minor amounts of phosphatidylglycerol, three unidentified glycolipids, two phosphoglycolipids and a phospholipid. Mycolic acids were present. These chemotaxonomic traits and the major fatty acids, which were C(16 : 1) cis9, C(16 : 0), C(18 : 1) and tuberculostearic acid (10-methyl C(18 : 0)), supported the affiliation of strain CC-12301(T) to the genus Gordonia. The results of physiological and biochemical tests allowed clear phenotypic differentiation of strain CC-12301(T) from the most closely related Gordonia species. Strain CC-12301(T) therefore represents a novel species, for which the name Gordonia humi sp. nov. is proposed, with the type strain CC-12301(T) (=DSM 45298(T) =CCM 7727(T)).

  5. Xenophilus aerolatus sp. nov., isolated from air.

    Science.gov (United States)

    Kim, Soo-Jin; Kim, Yi-Seul; Weon, Hang-Yeon; Anandham, Rangasamy; Noh, Hyung-Jun; Kwon, Soon-Wo

    2010-02-01

    A novel aerobic, Gram-negative, motile, rod-shaped bacterial strain designated 5516S-2(T) was isolated from an air sample taken in Suwon, Republic of Korea. Colonies were yellow-pigmented and circular with entire margins. 16S rRNA gene sequence analysis showed that strain 5516S-2(T) was closely related to Xylophilus ampelinus DSM 7250(T) (97.6 % sequence similarity), Variovorax soli KACC 11579(T) (97.5 %) and Xenophilus azovorans DSM 13620(T) (97.1 %). However, the phylogenetic tree indicated that strain 5516S-2(T) formed a separate clade from Xenophilus azovorans. Strain 5516S-2(T) displayed 42, 31 and 30 % DNA-DNA relatedness to the type strains of Xenophilus azovorans, Xylophilus ampelinus and V. soli, respectively. The major fatty acids (>10 % of total fatty acids) were C(16 : 0) (33.3 %), C(17 : 0) cyclo (18.8 %), C(18 : 1)omega7c (17.5 %) and summed feature 3 (comprising C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH; 13.0 %). The DNA G+C content was 69 mol%. The major quinone was ubiquinone Q-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminophospholipids. Genotypic and phenotypic characteristics clearly distinguished strain 5516S-2(T) from closely related species and indicated that it represents a novel species within the genus Xenophilus, for which the name Xenophilus aerolatus sp. nov. is proposed. The type strain is 5516S-2(T) (=KACC 12602(T)=DSM 19424(T)).

  6. Streptomyces xylanilyticus sp. nov., isolated from soil.

    Science.gov (United States)

    Moonmangmee, Duangtip; Kanchanasin, Pawina; Phongsopitanun, Wongsakorn; Tanasupawat, Somboon; Moonmangmee, Somporn

    2017-10-01

    A novel actinomycete, strain SR2-123 T , belonging to the genus Streptomyces, was isolated from a soil sample collected from the Sakaerat Environmental Research Station, Thailand Institute of Scientific and Technological Research, Nakhon Ratchasima Province, Thailand. The taxonomic position of the strain was characterized using a polyphasic study. Strain SR2-123 T contained ll-diaminopimelic acid, glucose, mannose and ribose in whole-cell hydrolysates. The N-acyl type of muramic acid was acetyl. Menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, an unknown phospholipid, unknown glycolipids, an unknown aminophospholipid, unknown lipids and an unknown aminolipid. The DNA G+C content was 74.8 mol%. The strain was closely related to Streptomyces coeruleorubidus JCM 4359 T (98.5 %), Streptomyces flavofungini JCM 4753 T (98.5 %), Streptomyces coerulescens NBRC 12758 T (98. 5 %) and Streptomyces alboflavus JCM 4615 T (98.4 %), based on 16S rRNA gene sequence similarities. The novel strain exhibited low DNA-DNA relatedness values with the type strains (11.4-25.0 %) of closely related species. On the basis of phenotypic and genotypic characteristics, strain SR2-123 T could be distinguished from closely related species of the genus Streptomyces and represents a novel species of the genus Streptomyces for which the name Streptomyces xylanilyticus sp. nov. is proposed. The type strain is SR2-123 T (=TISTR 2493 T =KCTC 39909 T ).

  7. Streptomyces krungchingensis sp. nov., isolated from soil.

    Science.gov (United States)

    Sripreechasak, Paranee; Phongsopitanun, Wongsakorn; Tamura, Tomohiko; Tanasupawat, Somboon

    2017-01-01

    A novel actinomycete, designated strain KC-035T, was isolated from soil collected from Krung Ching Waterfall National Park, Nakhon Si Thammarat Province, Thailand. Its taxonomic position was determined using a polyphasic approach. The strain had morphological and chemotaxonomic properties typical of members of the genus Streptomyces: flexuous spore chain; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H6) and MK-9(H4) as menaquinones; diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside as phospholipids; anteiso-C15 : 0, C16 : 0, iso-C16 : 0, iso-C15 : 0 and iso-C14 : 0 as major cellular fatty acids; and DNA G+C content of 72 mol%. 16S rRNA gene sequence analysis revealed that strain KC-035T showed high similarity to Streptomyces albiflavescens n20T (99.16 %) and Streptomyces siamensis KC-038T (98.43 %) as well as formed a monophyletic clade with them in the phylogenetic tree. On the basis of comparison of phenotypic properties and the low level of DNA-DNA relatedness, strain KC-035T could be distinguished from its closely related Streptomyces species and is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces krungchingensis sp. nov. is proposed. The type strain is KC-035T (=NBRC 110087T=KCTC 29503T=TISTR 2402T).

  8. Deinococcus antarcticus sp. nov., isolated from soil.

    Science.gov (United States)

    Dong, Ning; Li, Hui-Rong; Yuan, Meng; Zhang, Xiao-Hua; Yu, Yong

    2015-02-01

    A pink-pigmented, non-motile, coccoid bacterial strain, designated G3-6-20(T), was isolated from a soil sample collected in the Grove Mountains, East Antarctica. This strain was resistant to UV irradiation (810 J m(-2)) and slightly more sensitive to desiccation as compared with Deinococcus radiodurans. Phylogenetic analyses based on the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus. Highest sequence similarities were with Deinococcus ficus CC-FR2-10(T) (93.5 %), Deinococcus xinjiangensis X-82(T) (92.8 %), Deinococcus indicus Wt/1a(T) (92.5 %), Deinococcus daejeonensis MJ27(T) (92.3 %), Deinococcus wulumuqiensis R-12(T) (92.3 %), Deinococcus aquaticus PB314(T) (92.2 %) and Deinococcus radiodurans DSM 20539(T) (92.2 %). Major fatty acids were C18 : 1ω7c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), anteiso-C15 : 0 and C16 : 0. The G+C content of the genomic DNA of strain G3-6-20(T) was 63.1 mol%. Menaquinone 8 (MK-8) was the predominant respiratory quinone. Based on its phylogenetic position, and chemotaxonomic and phenotypic characteristics, strain G3-6-20(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus antarcticus sp. nov. is proposed. The type strain is G3-6-20(T) ( = DSM 27864(T) = CCTCC AB 2013263(T)). © 2015 IUMS.

  9. Pedobacterpsychrotolerans sp. nov., isolated from soil.

    Science.gov (United States)

    Manandhar, Pooja; Zhang, Gengxin; Lama, Arun; Hu, Yilun; Gao, Feng

    2016-11-01

    A Gram-stain-negative, non-motile, light-pink-pigmented, aerobic, rod-shaped bacterium, designated V5RDT, was isolated from soil of Damxung county in the Qinghai-Tibetan Plateau. Strain V5RDT grew luxuriously at 10 °C, at pH 9.0 and in the presence of 1 % NaCl (w/v). Phylogenetic analysis of 16S rRNA gene sequences placed strain V5RDT in the genus Pedobacter and found that it was most closely related to Pedobacter alluvionis DSM 19624T (97.3 %), Pedobacter ginsenosidimutans JCM 16721T (96.84 %), Pedobacter agri DSM 19486T (96.28 %), Pedobacter roseus JCM 13399T (96.22 %), Pedobacter sandarakinus KCTC 12559T (95.92 %) and Pedobacter borealis DSM 19626T (95.85 %). The G+C content of the genomic DNA of the type strain V5RDT was 37.40 mol%. DNA-DNA relatedness for the type strain V5RDT with respect to its closest phylogenetic relative, P. alluvionis DSM 19624T, was 62.5±1.7 %. The polar lipid profile of the strain consisted of phosphatidylethanolamine, one unidentified aminolipid, one unidentified glycolipid and two unidentified polar lipids. Menaquinone MK-7 was the predominant respiratory quinone, and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH were the major fatty acids. With respect to phenotypic characteristics, biochemical properties and phylogenetic inference, strain V5RDT represents a novel species of the genus Pedobacter, for which the name Pedobacter psychrotolerans sp. nov is proposed. The type strain is V5RDT (=CGMCC 1.15644T=DSM 103236T).

  10. Impact of serum SP-A and SP-D levels on comparison and prognosis of idiopathic pulmonary fibrosis

    Science.gov (United States)

    Wang, Kai; Ju, Qing; Cao, Jing; Tang, Wenze; Zhang, Jian

    2017-01-01

    Abstract Background and objective: Idiopathic pulmonary fibrosis (IPF) has a poor prognosis in general; however, it is heterogeneous to detect relative biomarkers for predicting the disease progression. Serum biomarkers can be conveniently collected to detect and help to differentially diagnose IPF and predict IPF prognosis. This meta-analysis aimed to evaluate the use of serum surfactant proteins A and D (SP-A and SP-D) for differential diagnosis and prognosis of IPF. Methods: Relevant articles were searched in PubMed, Embase, and Chinese National Knowledge Infrastructure databases and reviewed by 2 independent readers. Standard mean difference (SMD) and 95% confidence interval (CI) were calculated to assess the difference in serum levels of SP-A/D among patients with IPF, when compared to patients with non-IPF interstitial lung disease (ILD), pulmonary infection, and healthy control. Hazard ratio (HR) and 95% CI were used to compare the relative risk of mortality. Results: Twenty-one articles (totalling 1289 IPF patients) were included in final meta-analysis. Serum SP-A levels were significantly higher in patients with IPF than in patients with non-IPF ILD (SMD: 1.108 [0.584, 1.632], P infection (SMD: 1.320 [0.999, 1.640], P SMD: 2.802 [1.901, 3.702], P SMD: 0.459 [−0.000, 0.919], P = .050). Serum SP-D levels were significantly higher in patients with IPF than in patients with pulmonary infection (SMD: 1.308 [0.813, 1.803], P SMD: 2.235 [1.739, 2.731], P < .001). Risk of death in patients with IPF and elevated serum SP-A was increased 39% compared to patients with low SP-A groups. Elevated SP-D increased risk by 111% when compared to low SP-D. In acute exacerbation of IPF, serum SP-A/D were higher than those in stable stage. The comparisons and prognosis might be different in Asian and Caucasian patients. Conclusions: Serum SP-A/D detection might be useful for differential diagnosis and prediction of survival in patients with IPF. PMID:28591049

  11. Flow chemistry as a discovery tool to access sp2-sp3 cross-coupling reactions via diazo compounds.

    Science.gov (United States)

    Tran, Duc N; Battilocchio, Claudio; Lou, Shing-Bong; Hawkins, Joel M; Ley, Steven V

    2015-02-01

    The work takes advantage of an important feature of flow chemistry, whereby the generation of a transient species (or reactive intermediate) can be followed by a transfer step into another chemical environment, before the intermediate is reacted with a coupling partner. This concept is successfully applied to achieve a room temperature sp 2 -sp 3 cross coupling of boronic acids with diazo compounds, these latter species being generated from hydrazones under flow conditions using MnO 2 as the oxidant.

  12. Modular Stereoselective Synthesis of (1 -> 2)-C-Glycosides based on the sp(2)-sp(3) Suzuki-Miyaura Reaction

    Czech Academy of Sciences Publication Activity Database

    Oroszová, B.; Choutka, J.; Pohl, Radek; Parkan, K.

    2015-01-01

    Roč. 21, č. 19 (2015), s. 7043-7047 ISSN 0947-6539 Grant - others:GA ČR(CZ) GPP207/12/P713; GA ČR(CZ) GA15-17572S Institutional support: RVO:61388963 Keywords : C-disaccharides * C-glycosides * diastereoselectivity * Mitsunobu reaction * sp(2)-sp(3) coupling Subject RIV: CC - Organic Chemistry Impact factor: 5.771, year: 2015

  13. Draft Genome Sequence of Deep-Sea Alteromonas sp. Strain V450 Isolated from the Marine Sponge Leiodermatium sp.

    Science.gov (United States)

    Wang, Guojun; Barrett, Nolan H; McCarthy, Peter J

    2017-02-02

    The proteobacterium Alteromonas sp. strain V450 was isolated from the Atlantic deep-sea sponge Leiodermatium sp. Here, we report the draft genome sequence of this strain, with a genome size of approx. 4.39 Mb and a G+C content of 44.01%. The results will aid deep-sea microbial ecology, evolution, and sponge-microbe association studies. Copyright © 2017 Wang et al.

  14. Impact of serum SP-A and SP-D levels on comparison and prognosis of idiopathic pulmonary fibrosis

    OpenAIRE

    Wang, Kai; Ju, Qing; Cao, Jing; Tang, Wenze; Zhang, Jian

    2017-01-01

    Abstract Background and objective: Idiopathic pulmonary fibrosis (IPF) has a poor prognosis in general; however, it is heterogeneous to detect relative biomarkers for predicting the disease progression. Serum biomarkers can be conveniently collected to detect and help to differentially diagnose IPF and predict IPF prognosis. This meta-analysis aimed to evaluate the use of serum surfactant proteins A and D (SP-A and SP-D) for differential diagnosis and prognosis of IPF. Methods: Relevant artic...

  15. Uji Aktivitas Antibakteri Ekstrak Daun Mayana Jantan (Coleus Atropurpureus Benth) Terhadap Pertumbuhan Bakteri Streptococcus SP. Dan Pseudomonas SP.

    OpenAIRE

    Muljono, Patrick; Fatimawali; Manampiring, Aaltje E

    2016-01-01

    : The painted nettle (Coleus atropurpureus benth) is a plant that is known to have medicinal properties, especially the leaf. The active compounds that are contained in the leafs are thought to work as an antibacterial. This study aims to measure the inhibitory strength of painted nettle leaf (Coleus atropurpureus benth) extract against the growth of Streptococcus sp. and Pseudomonas sp. This was an experimental laboratory study. The polar extract of painted nettle leaf (Coleus atropurpureus ...

  16. Superhard sp{sup 2}–sp{sup 3} hybrid carbon allotropes with tunable electronic properties

    Energy Technology Data Exchange (ETDEWEB)

    Hu, Meng; Ma, Mengdong; Zhao, Zhisheng; Yu, Dongli; He, Julong, E-mail: hjl@ysu.edu.cn [State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004 (China)

    2016-05-15

    Four sp{sup 2}–sp{sup 3} hybrid carbon allotropes are proposed on the basis of first principles calculations. These four carbon allotropes are energetically more favorable than graphite under suitable pressure conditions. They can be assembled from graphite through intralayer wrinkling and interlayer buckling, which is similar to the formation of diamond from graphite. For one of the sp{sup 2}–sp{sup 3} hybrid carbon allotropes, mC24, the electron diffraction patterns match these of i-carbon, which is synthesized from shock-compressed graphite (H. Hirai and K. Kondo, Science, 1991, 253, 772). The allotropes exhibit tunable electronic characteristics from metallic to semiconductive with band gaps comparable to those of silicon allotropes. They are all superhard materials with Vickers hardness values comparable to that of cubic BN. The sp{sup 2}–sp{sup 3} hybrid carbon allotroes are promising materials for photovoltaic electronic devices, and abrasive and grinding tools.

  17. Botanical pesticides effect from shells of bean’s cashew nut on biological agents of trichoderma sp. and gliocladium sp.

    Science.gov (United States)

    Bande, L. O. S.; Mariadi; Gusnawaty, HS; Nuriadi; Trisulpa, L.; Rahmania

    2018-02-01

    A shell of cashew nut (Anacardium occidentanle) has contained Cashew Nut Shell Liquid (CNSL) that is used as botanical pesticides. CNSL oil consists of active substance such as anacardat acid, cardol and cardanol. Utilization of the pesticides from shells of cashew nut to control pests and diseases of plants would be affected on biological agents. The objective of this research was to investigate pesticides inhibition on the increase of mycelium Trichoderma sp. and Gliocladium sp. by in vitro method. The tested concentration sample consisted of 0.0% (control), 2.5%, 7.5% and 10.0% in PDA media. The results of this research showed that 2.5% botanical pesticides concentration could minimize mycelium of Trichoderma sp. and Gliocladium sp. 22.73% and 21.04% respectively and also the increase shells of cashew extract could be affected the increase of mycelium inhibition. The extract with 2.5% concentration was the recommended concentration to control of fruit rot diseases and if concentration was 10.0% then its inhibition become 54.98% and 49.35%, respectively. The results proved that uncontrolled utilization of the pesticides could be affected on decrease of Trichoderma sp. and Gliocladium sp. growth.

  18. Characterization of Ethanolic Extract of Streptomyces sp. as a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12

    Directory of Open Access Journals (Sweden)

    Lenni Fitri

    2017-07-01

    Full Text Available Endophytic Streptomyces sp. AEBg12 isolated from Zingiber cassumunar (Bangle is known to produce pancreatic lipase inhibitory compound. However, the characteristics of this active compound has not been reported yet. This study aimed to determine the characteristics of pancreatics inhibitory compound produced by Streptomyces sp. AEBg12 and to assess the role of endophytic actinobacteria in producing pancreatic lipase inhibitor using endophytic-free bangle tissue culture, wild bangle and compared with the activity of Streptomyces sp. AEBg12 endophytes. Supernatant of Streptomyces sp. AEBg12 was extracted using ethanol, ethyl acetate, and n-hexane solvents. Toxicity test was performed using larvae of shrimp Artemia salina. The results showed that the best solvent to obtain pancreatic lipase inhibitor compounds was ethanol. Phytochemical analysis showed that ethanolic extract of endophytic Streptomyces sp. AEBg12 contained flavonoids. IC50 value of ethanol extract was 180.83 µg/ml. The result of TLC showed that ethanolic extract of Streptomyces AEBg12 had a blue luminescence band indicated that there were either flavone, flavanones, flavonols or isoflavones. Inhibitory activity of Streptomyces sp. AEBg12 was higher than wild bangle and bangle tissue culture. The information from this study can be be used as a basic data for further characterization of the active compound, which might be developed as an antiobesity agent through its pancreatic lipase inhibitory activity.

  19. UJI POTENSI Gliocladium sp TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN TOMAT

    Directory of Open Access Journals (Sweden)

    Lina Herlina

    2013-09-01

    Full Text Available AbstrakMikroorganisme tanah seperti Gliocladium sp dapat bertindak sebagai dekomposer dan juga sebagai agen pengendali hayati patogen tanaman hal ini memberikan harapan untuk mengurangi penggunaan pupuk dan fungisida sintetik. Tujuan penelitian untuk menguji potensi biofertililzer Gliocladium sp terhadap pertumbuhan dan produksi buah tomat. Variabel bebas yaitu bioferlizer Gliocladium sp  dengan dosis (g/tanaman 0,50, 100, 150, dan 200 .Variabel tergantung adalah pertumbuhan dan produksi tanaman tomat. Parameter pertumbuhan yang diukur adalah  tinggi tanaman, kandungan klorofil a dan b. Parameter hasil tanaman yang diukur adalah berat buah setelah panen. Hasil uji Anava satu jalan menunjukkan bahwa pemberian Gliocladium sp perpengaruh terhadap tinggi tanaman, kandungan klorofil a dan klorofil b, berat tomat  . Hasil uji lanjut Duncan menunjukkan bahwa dosis 100, g 150 g dan 200 g tidak berbeda signifikan terhadap tinggi tanaman, dosis 150g dan 200 g tidak berbedan nyata dalam pembentukan klorofil a dan klorofil b. Pemberian Gliocladium dosis 150g paling tinggi terhadap berat buah. Simpulan dalam penelitian ini bahwa pemberian Gliokompos sp berpengaruh terhadap pertumbuhan dan produksi buah tomat. AbstractSoil microorganisms such as Gliocladium sp. can play role in the decomposition and can also become the biological control of pathogenic plants. The animal is potentially reduce the use of fertilizers and synthetic fungidid. The aim of the study was to test the potential effect of biofertililzer Gliocladium sp. on the growth and production of tomatoes. The independent variables was biofertilizer Gliocladium sp. at various dosages (g/plant of 0, 50, 100, 150, dan 200. The dependent variables were the growth and the production of tomatoes. The growth parameter measured were plant height, as well as a and b chlorophyl contents.  The crop parameter was the fruit weight post-harvest.  A one-way anova showed that Gliocladium sp. affected the

  20. Penggunaan Jamur Antagonis Trichoderma sp. dan Gliocladium sp. untuk Mengendalikan Penyakit Layu (Fusarium oxysporum) pada Tanaman Bawang Merah (Allium ascalonicum L.)

    OpenAIRE

    Ramadhina, Arie

    2015-01-01

    Arie Ramadhina, 2012. The Use of Antagonism Fungus of Trichoderma sp and Gliocladium sp. for Controlling Wilt (Fusarium oxysporum) in Red Onion Plants (Allium ascolanicum). Supervised by Lisnawita and Lahmuddin Lubis. The aim of the research was to know the effectiveness of antagonism fungus of Trichoderma sp. and Gliocladium sp. in controlling wilt in red onion plants. The research was performed in the green-house at the faculty of Agriculture, USU, from February until May, 2012. The researc...

  1. In Vitro Antimicrobial Potential of the Lichen Parmotrema sp. Extracts against Various Pathogens.

    Science.gov (United States)

    Chauhan, Ritika; Abraham, Jayanthi

    2013-07-01

    The ongoing increasing antibiotic resistance is one of the biggest challenges faced by global public health. The perennial need for new antimicrobials against a background of increasing antibiotic resistance in pathogenic and opportunistic microorganisms obliges the scientific community to constantly develop new drugs and antimicrobial agents. Lichens are known prolific sources of natural antimicrobial drugs and biologically active natural products. This study was aimed to explore in vitro antimicrobial activity of lichen Parmotrema sp. The methanol and aqueous extracts of lichen Parmotrema sp. was extracted using Soxhlet extractor. Antibiotic assessment of methanol and aqueous extracts was done against eight bacterial (Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Salmonella sp., Shigella sp., Enterococci faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniae,) clinical pathogens and five plant pathogenic fungal strains (Aspergillus terreus strain JAS1, Scedosporium sp. JAS1, Ganoderma sp. JAS4, Candida tropicalis and Fusarium sp.) by Kirby-Bauer method. The methanol lichen Parmotrema sp. extract inhibited all the test organisms. The highest antibacterial activity was found against Pseudomonas aeruginosa and Staphylococcus aureus. The weakest activity was manifested in Salmonella sp. and Scedosporium sp. JAS1. Strong antifungal effect was found against Ganoderma sp. JAS4 and Fusarium sp. The aqueous lichen Parmotrema sp. extract revealed neither antibacterial nor antifungal activity. The present study shows that tested lichen Parmotrema sp. extracts demonstrated a strong antimicrobial effect. That suggests the active components from methanol extracts of the investigated lichen Parmotrema sp. can be used as natural antimicrobial agent against pathogens.

  2. On the fourth Diadema species (Diadema-sp) from Japan.

    Science.gov (United States)

    Chow, Seinen; Kajigaya, Yoshikazu; Kurogi, Hiroaki; Niwa, Kentaro; Shibuno, Takuro; Nanami, Atsushi; Kiyomoto, Setuo

    2014-01-01

    Four long-spined sea urchin species in the genus Diadema are known to occur around the Japanese Archipelago. Three species (D. savignyi, D. setosum, and D. paucispinum) are widely distributed in the Indo-Pacific Ocean. The fourth species was detected by DNA analysis among samples originally collected as D. savignyi or D. setosum in Japan and the Marshall Islands and tentatively designated as Diadema-sp, remaining an undescribed species. We analyzed nucleotide sequences of the cytochrome oxidase I (COI) gene in the "D. savignyi-like" samples, and found all 17 individuals collected in the mainland of Japan (Sagami Bay and Kyushu) to be Diadema-sp, but all nine in the Ryukyu Archipelago (Okinawa and Ishigaki Islands) to be D. savignyi, with large nucleotide sequence difference between them (11.0%±1.7 SE). Diadema-sp and D. savignyi shared Y-shaped blue lines of iridophores along the interambulacrals, but individuals of Diadema-sp typically exhibited a conspicuous white streak at the fork of the Y-shaped blue iridophore lines, while this feature was absent in D. savignyi. Also, the central axis of the Y-shaped blue lines of iridophores was approximately twice as long as the V-component in D. savignyi whereas it was of similar length in Diadema-sp. Two parallel lines were observed to constitute the central axis of the Y-shaped blue lines in both species, but these were considerably narrower in Diadema-sp. Despite marked morphological and genetic differences, it appears that Diadema-sp has been mis-identified as D. savignyi for more than half a century.

  3. On the fourth Diadema species (Diadema-sp from Japan.

    Directory of Open Access Journals (Sweden)

    Seinen Chow

    Full Text Available Four long-spined sea urchin species in the genus Diadema are known to occur around the Japanese Archipelago. Three species (D. savignyi, D. setosum, and D. paucispinum are widely distributed in the Indo-Pacific Ocean. The fourth species was detected by DNA analysis among samples originally collected as D. savignyi or D. setosum in Japan and the Marshall Islands and tentatively designated as Diadema-sp, remaining an undescribed species. We analyzed nucleotide sequences of the cytochrome oxidase I (COI gene in the "D. savignyi-like" samples, and found all 17 individuals collected in the mainland of Japan (Sagami Bay and Kyushu to be Diadema-sp, but all nine in the Ryukyu Archipelago (Okinawa and Ishigaki Islands to be D. savignyi, with large nucleotide sequence difference between them (11.0%±1.7 SE. Diadema-sp and D. savignyi shared Y-shaped blue lines of iridophores along the interambulacrals, but individuals of Diadema-sp typically exhibited a conspicuous white streak at the fork of the Y-shaped blue iridophore lines, while this feature was absent in D. savignyi. Also, the central axis of the Y-shaped blue lines of iridophores was approximately twice as long as the V-component in D. savignyi whereas it was of similar length in Diadema-sp. Two parallel lines were observed to constitute the central axis of the Y-shaped blue lines in both species, but these were considerably narrower in Diadema-sp. Despite marked morphological and genetic differences, it appears that Diadema-sp has been mis-identified as D. savignyi for more than half a century.

  4. Development of Streptomyces sp. FR-008 as an emerging chassis

    Directory of Open Access Journals (Sweden)

    Qian Liu

    2016-09-01

    Full Text Available Microbial-derived natural products are important in both the pharmaceutical industry and academic research. As the metabolic potential of original producer especially Streptomyces is often limited by slow growth rate, complicated cultivation profile, and unfeasible genetic manipulation, so exploring a Streptomyces as a super industrial chassis is valuable and urgent. Streptomyces sp. FR-008 is a fast-growing microorganism and can also produce a considerable amount of macrolide candicidin via modular polyketide synthase. In this study, we evaluated Streptomyces sp. FR-008 as a potential industrial-production chassis. First, PacBio sequencing and transcriptome analyses indicated that the Streptomyces sp. FR-008 genome size is 7.26 Mb, which represents one of the smallest of currently sequenced Streptomyces genomes. In addition, we simplified the conjugation procedure without heat-shock and pre-germination treatments but with high conjugation efficiency, suggesting it is inherently capable of accepting heterologous DNA. In addition, a series of promoters selected from literatures was assessed based on GusA activity in Streptomyces sp. FR-008. Compared with the common used promoter ermE*-p, the strength of these promoters comprise a library with a constitutive range of 60–860%, thus providing the useful regulatory elements for future genetic engineering purpose. In order to minimum the genome, we also target deleted three endogenous polyketide synthase (PKS gene clusters to generate a mutant LQ3. LQ3 is thus an “updated” version of Streptomyces sp. FR-008, producing fewer secondary metabolites profiles than Streptomyces sp. FR-008. We believe this work could facilitate further development of Streptomyces sp. FR-008 for use in biotechnological applications.

  5. Allophycocyanin forms isolated from Nostoc sp. phycobilisomes

    Energy Technology Data Exchange (ETDEWEB)

    Zilinskas, B.A.; Zimmerman, B.K.; Gantt, E.

    1978-01-01

    Allophycocyanin from dissociated phycobilisomes of Nostoc sp. occurs in three spectrally identifiable forms that fractionate on calcium phosphate adsorption chromatography as: allophycocyanin (APC) I (15 to 20%), APC II (40 to 50%), and APC III (30 to 40%). APC I has a single absorption maximum at 654 nm, and a fluorescence emission peak at 678 nm. The absorption peaks of APC II and III are both at 650 nm, but the relative absorbance at 620/650 nm of APC III is less than that of APC II. The emission of both is maximum at 660 nm. On zone sedimentation in sucrose, their S/sub 20 w/ values of 6.0 +- 0.1 (APC I), 5.0 +- 0.1 (APC II), and 5.3 +-0.2 (APC III) were comparable to the order of their elution from Sephadex G-200. On SDS acrylamide gel electrophoresis two subunits were resolved with apparent molecular weights of 16,900 and 18,400 daltons. When stained by Coomassie blue, they were present in a ratio of 1..cap alpha..:1..beta.. in APC II and III, and a probable ratio of 2..cap alpha..:3..beta.. in APC I. The larger size of APC I may be accounted for by additional ..beta.. subunits, by the presence of an additional polypeptide of 35,000 daltons, or both. Over several days, bleaching as noted by a decrease in absorbance at 650 nm, occurred in all three forms; in addition, the more pronounced bleaching at 650 nm, relative to 620 nm, results in APC III becoming spectrally identical to APC II. A trace of a fourth pigment, probably comparable to allophycocyanin-B, was occasionally detected. The results suggest that several in vitro APC forms (sharing similar subunits) arise upon phycobilisome dissociation, and that APC I is the form most closely related to the final fluorescence emitter of intact phycobilisomes. In this form it probably serves as the bridging pigment in energy transfer from the phycobilisomes to chlorophyll.

  6. Occurrence of Pyemotes sp. on Tuta absoluta (Meyrick

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    Carlos Romero Ferreira de Oliveira

    2007-11-01

    Full Text Available The aim of this work was to study the population reduction of the tomato leafminer moth, Tuta absoluta (Meyrick, by Pyemotes sp. in the laboratory. The mite became greenish when fed on caterpillars, pupae, and adults of T. absoluta. The caterpillars and adults of T. absoluta were quickly paralyzed by the mite venom. A single T. absoluta could host many Pyemotes sp, physogastric females, which were allowed to grow on the moth. Pyemotes sp. can be a new alternative for the biological control of T. absoluta. However, this possibility must be better understood before it could be recommended, because Pyemotes sp. could also cause dermatitis in the humans.O ácaro Pyemotes sp. (Acari: Pyemotidae é um parasita de várias espécies de insetos e exibe o processo de fisogastria na reprodução. É registrada a ocorrência de Pyemotes sp. sobre a traça-do-tomateiro, Tuta absoluta (Meyrick, que sofreu redução considerável de suas populações em condições de laboratório. Este ácaro foi observado parasitando larvas, pupas e adultos de T. absoluta e suas fêmeas fisogástricas apresentaram coloração esverdeada. As larvas e adultos de T. absoluta atacados por Pyemotes sp. exibiram paralisia em poucos minutos, devido à injeção de toxinas por parte do ácaro. Observou-se que um único indivíduo de T. absoluta pode suportar muitas fêmeas fisogástricas de Pyemotes sp., permitindo que outra geração do ácaro continuasse a se reproduzir. O ácaro Pyemotes sp. surge como uma possibilidade para o controle biológico de T. absoluta, mas sua utilização deve ser melhor avaliada já que é uma espécie causadora de dermatites em humanos.

  7. Analysis of polyhydroxyalkanoates granuales in bacillus Sp. MFD11 and enterobacter Sp. SEL2

    International Nuclear Information System (INIS)

    Naheed, N.; Jamil, N.

    2016-01-01

    Bacillus sp. MFD11 (JF901809) and Enterobacter sp. SEL2 (JF901810) were isolated from agriculture waste contaminated sites. When fed with 2% glucose as a carbon source, these bacteria produced 75.26±0.45% and 76.61±0.28% PHA of their wet weight respectively. The accumulated PHA was extracted by direct addition of sodium dodysyl sulphate in the culture medium, which yielded 52.3±0.56 micro g/l and 136.21±0.45 micro g/l PHA respectively when assayed with Crotonic acid. The PHA detection medium (PDM) provided nutrient limitation condition which favored accumulation of PHA granules. A tremendous increase in cell size was observed when strain MFD11 was grown in PDM. The size of the granules as revealed by TEM micrographs spanned from 0.1 to 1.5 micro m which is quite large as compared to the size reported in the literature 0.2 to 0.5 micro m 18). (PHA polymer was analyzed by FTIR, GC/MS and proton Nuclear magnetic resonance. The intense absorption band in the spectrum at 1724-1740 cm -1 and 1215 cm -1 to 1280 corresponding to C=O and C-O stretching group, respectively, indicated that the both strains were PHA producers. GC/MS analysis indicated that the polymer produced were copolymers of PHB-co-PHV. NMR also suggested that the extracted PHA was not a homopolymer but was the blend of copolymers with 3HV in lower abundance. Differential calorimetric thermal analysis showed melting temperature of 163 and 169 degree C for PHA produced by both strains, respectively. However, the observed melting temperature was found to be lower than the standard PHB (Signa-aldrich). (author)

  8. CULTIVO EXPERIMENTAL DEL CLADOCERO Moina sp ALIMENTADO CON Ankistrodesmus sp y Saccharomyces cereviseae

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    Martha Prieto

    2006-05-01

    Full Text Available Saccharomyces cereviseae. Materiales y métodos. Fueron realizados cultivos experimentales de la cepade cladóceros Moina sp en el laboratorio de Alimento Vivo de la Universidad de Córdoba, bajo condicionescontroladas de temperatura (22oC, pH (7.6, intensidad lumínica (2000 lux y aireación. Se emplearondos dietas de alimento como tratamiento (Ankistrodesmus y Ankistrodesmus mas Saccharomyces cereviseaeen concentración de 40 x 105 cel.ml-1 cada uno, para determinar su efecto sobre el desempeño de lapoblación. Diariamente se registró la densidad poblacional y celular. Resultados. Se obtuvieron diferenciassignificativas para el efecto de las dietas sobre el crecimiento poblacional de los cladóceros, los individuosalimentados con Ankistrodesmus sp + Saccharomyces cereviseae alcanzaron una densidad de 12.3a±0.30 org/ml-1 presentando mayor tasa instantánea de crecimiento (K 0.36 ± 0.002, el menor tiempode duplicación (td 1.94 ± 0.012 y el mayor rendimiento (r 1,1 ± 0.07 clad.ml-1.día-1. Conclusión. Loscladóceros por sus características de crecimiento en cultivo, presentan adaptación favorable a lascondiciones de manejo para la producción de biomasas potencialmente útiles como partícula nutritivacon fines acuícolas.

  9. Pseudomonas helleri sp. nov. and Pseudomonas weihenstephanensis sp. nov., isolated from raw cow's milk.

    Science.gov (United States)

    von Neubeck, M; Huptas, C; Glück, C; Krewinkel, M; Stoeckel, M; Stressler, T; Fischer, L; Hinrichs, J; Scherer, S; Wenning, M

    2016-03-01

    Analysis of the microbiota of raw cow's milk and semi-finished milk products yielded seven isolates assigned to the genus Pseudomonas that formed two individual groups in a phylogenetic analysis based on partial rpoD and 16S rRNA gene sequences. The two groups could be differentiated from each other and also from their closest relatives as well as from the type species Pseudomonas aeruginosa by phenotypic and chemotaxonomic characterization and average nucleotide identity (ANIb) values calculated from draft genome assemblies. ANIb values within the groups were higher than 97.3 %, whereas similarity values to the closest relatives were 85 % or less. The major cellular lipids of strains WS4917T and WS4993T were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major quinone was Q-9 in both strains, with small amounts of Q-8 in strain WS4917T. The DNA G+C contents of strains WS4917T and WS4993T were 58.08 and 57.30 mol%, respectively. Based on these data, strains WS4917T, WS4995 ( = DSM 29141 = LMG 28434), WS4999, WS5001 and WS5002 should be considered as representatives of a novel species of the genus Pseudomonas, for which the name Pseudomonas helleri sp. nov. is proposed. The type strain of Pseudomonas helleri is strain WS4917T ( = DSM 29165T = LMG 28433T). Strains WS4993T and WS4994 ( = DSM 29140 = LMG 28438) should be recognized as representing a second novel species of the genus Pseudomonas, for which the name Pseudomonas weihenstephanensis sp. nov. is proposed. The type strain of Pseudomonas weihenstephanensis is strain WS4993T ( = DSM 29166T = LMG 28437T).

  10. Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk.

    Science.gov (United States)

    von Neubeck, Mario; Huptas, Christopher; Glück, Claudia; Krewinkel, Manuel; Stoeckel, Marina; Stressler, Timo; Fischer, Lutz; Hinrichs, Jörg; Scherer, Siegfried; Wenning, Mareike

    2017-06-01

    Five strains, designated WS 4672T, WS 4998, WS 4992T, WS 4997 and WS 5000, isolated from bovine raw milk formed two individual groups in a phylogenetic analysis. The most similar species on the basis of 16S rRNA gene sequences were Pseudomonas azotoformans IAM 1603T, Pseudomonas gessardii CIP 105469T and Pseudomonas libanensis CIP 105460T showing 99.7-99.6 % similarity. Using rpoD gene sequences Pseudomonas veronii LMG 17761T (93.3 %) was most closely related to strain WS 4672T and Pseudomonas libanensis CIP 105460T to strain WS 4992T (93.3 %). The five strains could be differentiated from their closest relatives and from each other by phenotypic and chemotaxonomic characterization and ANIb values calculated from draft genome assemblies. ANIb values of strains WS 4992T and WS4671T to the closest relatives are lower than 90 %. The major cellular polar lipids of both strains are phosphatidylethanolamine, phosphatidylglycerol, a phospholipid and diphosphatidylglycerol, and their major quinone is Q-9. The DNA G+C content of strains WS 4992T and WS 4672T were 60.0  and 59.7  mol%, respectively. Based on these genotypic and phenotypic traits two novel species of the genus Pseudomonas are proposed: Pseudomonas lactis sp. nov. [with type strain WS 4992T (=DSM 29167T=LMG 28435T) and the additional strains WS 4997 and WS 5000], and Pseudomonasparalactis sp. nov. [with type strain WS 4672T (=DSM 29164T=LMG 28439T) and additional strain WS 4998].

  11. CAPACIDAD ANTAGONISTA DE HONGOS CELULOLÍTICOS FRENTE A Fusarium sp. Y Macrophomina sp.

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    Néstor Leopoldo Tarazona Meza

    2014-12-01

    Full Text Available El objetivo de esta investigación fue evaluar la capacidad antagónica de ocho hongos celulolíticos nativos frente a los hongos fitopatógenos Fusarium sp. y Macrophomina sp. En primera instancia se determinó la cepa promisoria del hongo celulolítico, para lo cual, en caja Petri se colocó un disco de agar de 4 mm de diámetro con micelio de una cepa antagonista y en el extremo opuesto, a una distancia de cinco centímetros aproximadamente, otro disco de 4 mm con micelio de un patógeno. La capacidad antagónica se determinó por el grado de micoparasitismo y la competencia por nutrientes y espacios, registrando el porcentaje de inhibición del crecimiento radial. Con la cepa del hongo celulolítico que tuvo el mejor comportamiento antagonista se realizó un cocultivo independiente con cada patógeno, aquí se evalúo las UFC, crecidas en medio PDA. También se inoculó junto a las cepas fitopatógenas en plantas de pimiento para conocer el nivel de antagonismo en macetas. En la evaluación cualitativa, la cepa A.O-5 mostró una invasión y esporulación total en la superficie en ambos patógenos; mientras que en el cocultivo inhibe el crecimiento de los patógenos a las 96 h. Igualmente demostró su acción antagonista frente a Fusarium y Macrophomina en las plántulas de pimiento. Por lo encontrado, se estima que la cepa A.O-5 es un potencial agente antagonista de los fitopatógenos evaluados.

  12. Streptomyces rhizosphaerihabitans sp. nov. and Streptomyces adustus sp. nov., isolated from bamboo forest soil.

    Science.gov (United States)

    Lee, Hyo-Jin; Whang, Kyung-Sook

    2016-09-01

    Three novel isolates belonging to the genus Streptomyces, designated JR-35T, JR-46 and WH-9T, were isolated from bamboo forest soil in Damyang, Korea. The 16S rRNA gene sequences of strains JR-35T and JR-46 showed highest similarities with Streptomyces olivochromogenes NBRC 3178T (99.1 %), Streptomyces siamensis KC-038T (98.9 %), Streptomyces chartreusis NBRC 12753T (98.9 %), Streptomyces resistomycificus NRRL ISP-5133T (98.9 %) and Streptomyces bobili JCM 4627T (98.8 %), and strain WH-9Tshowed highest sequence similarities with Streptomyces. bobili JCM 4627T (99.2 %), Streptomyces phaeoluteigriseus NRRL ISP-5182T (99.2 %), Streptomyces alboniger NBRC 12738T (99.2 %), Streptomyces galilaeus JCM 4757T (99.1 %) and Streptomyces pseudovenezuelae NBRC 12904T (99.1 %). The predominant menaquinones were MK-9 (H6) and MK-9 (H8). The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, iso-C14 : 0 and iso-C15 : 0 for strains JR-35T and JR-46 and anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0 for strain WH-9T. The G+C content of the genomic DNA of strains JR-35T, JR-46 and WH-9T were 69.4, 74.4 and 74.1 mol%, respectively. Based on the phenotypic and genotypic data, the three strains are assigned to two novel species of the genus Streptomyces, for which the names Streptomyces rhizosphaerihabitans sp. nov. (type stain JR-35T=KACC 17181T=NBRC 109807T) and Streptomyces adustus sp. nov. (type strain WH-9T=KACC 17197T=NBRC 109810T) are proposed.

  13. Serratia myotis sp. nov. and Serratia vespertilionis sp. nov., isolated from bats hibernating in caves.

    Science.gov (United States)

    García-Fraile, P; Chudíčková, M; Benada, O; Pikula, J; Kolařík, M

    2015-01-01

    During the study of bacteria associated with bats affected by white-nose syndrome hibernating in caves in the Czech Republic, we isolated two facultatively anaerobic, Gram-stain-negative bacteria, designated strains 12(T) and 52(T). Strains 12(T) and 52(T) were motile, rod-like bacteria (0.5-0.6 µm in diameter; 1-1.3 µm long), with optimal growth at 20-35 °C and pH 6-8. On the basis of the almost complete sequence of their 16S rRNA genes they should be classified within the genus Serratia; the closest relatives to strains 12(T) and 52(T) were Serratia quinivorans DSM 4597(T) (99.5 % similarity in 16S rRNA gene sequences) and Serratia ficaria DSM 4569(T) (99.5% similarity in 16S rRNA gene sequences), respectively. DNA-DNA relatedness between strain 12(T) and S. quinivorans DSM 4597(T) was only 37.1% and between strain 52(T) and S. ficaria DSM 4569(T) was only 56.2%. Both values are far below the 70% threshold value for species delineation. In view of these data, we propose the inclusion of the two isolates in the genus Serratia as representatives of Serratia myotis sp. nov. (type strain 12(T) =CECT 8594(T) =DSM 28726(T)) and Serratia vespertilionis sp. nov. (type strain 52(T) =CECT 8595(T) =DSM 28727(T)). © 2015 IUMS.

  14. Description of Leifsonia kafniensis sp. nov. and Leifsonia antarctica sp. nov.

    Science.gov (United States)

    Pindi, Pavan Kumar; Kishore, K Hara; Reddy, G S N; Shivaji, S

    2009-06-01

    Strains KFC-22(T) and SPC-20(T) are yellow-pigmented, Gram-positive, aerobic, non-motile, rod-shaped bacteria that were isolated from a soil sample near the Kafni glacier in the Himalayan mountain ranges in India, and from a spade core sediment sample from the Antarctic Ocean at Larsemann Hill, respectively. In both cases, the cell-wall peptidoglycan contained 2,4-diaminobutyric acid as the diamino acid, anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0) were the predominant fatty acids and MK-11 was the major isoprenoid quinone in the cell membrane. On the basis of the above-mentioned characteristics, both strains can be assigned to the genus Leifsonia. The strains share 16S rRNA gene sequence similarity of 97.7 % and DNA relatedness of only 10 %, indicating that they represent different species. A blast analysis indicated that Leifsonia pindariensis PON10(T) was the closest phylogenetic neighbour of strains SPC-20(T) and KFC-22(T), showing 16S rRNA gene sequence similarities of 97.3 and 97.7 %, respectively. However, at the whole-genome level, strains KFC-22(T) and SPC-20(T) shared 42 and 11 % DNA-DNA relatedness, respectively, with L. pindariensis PON10(T). In addition, both strains exhibited several phenotypic differences with respect to L. pindariensis PON10(T). Thus, on the basis of the differences that the two strains exhibited with respect to L. pindariensis, both were identified as representing novel species of the genus Leifsonia, for which the names Leifsonia kafniensis sp. nov. (type strain KFC-22(T) =NCCB 100216(T) =LMG 24362(T)) and Leifsonia antarctica sp. nov. (type strain SPC-20(T) =NCCB 100227(T) =LMG 24541(T)) are proposed.

  15. Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov.

    Science.gov (United States)

    Nouioui, Imen; Sangal, Vartul; Carro, Lorena; Teramoto, Kanae; Jando, Marlen; Montero-Calasanz, Maria Del Carmen; Igual, José Mariano; Sutcliffe, Iain; Goodfellow, Michael; Klenk, Hans-Peter

    2017-12-01

    Two rapidly growing mycobacteria with identical 16S rRNA gene sequences were the subject of a polyphasic taxonomic study. The strains formed a well-supported subclade in the mycobacterial 16S rRNA gene tree and were most closely associated with the type strain of Mycobacterium novocastrense. Single and multilocus sequence analyses based on hsp65, rpoB and 16S rRNA gene sequences showed that strains SN 1900 T and SN 1904 T are phylogenetically distinct but share several chemotaxonomic and phenotypic features that are are consistent with their classification in the genus Mycobacterium. The two strains were distinguished by their different fatty acid and mycolic acid profiles, and by a combination of phenotypic features. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values for strains SN 1900 T and SN 1904 T were 61.0 % and 94.7 %, respectively; in turn, the corresponding dDDH and ANI values with M. novocastrense DSM 44203 T were 41.4 % and 42.8 % and 89.3 % and 89.5 %, respectively. These results show that strains SN1900 T and SN 1904 T form new centres of taxonomic variation within the genus Mycobacterium. Consequently, strains SN 1900 T (40 T =CECT 8763 T =DSM 43219 T ) and SN 1904 T (2409 T =CECT 8766 T =DSM 43532 T ) are considered to represent novel species, for which the names Mycobacteriumlehmannii sp. nov. and Mycobacteriumneumannii sp. nov. are proposed. A strain designated as 'Mycobacteriumacapulsensis' was shown to be a bona fide member of the putative novel species, M. lehmannii.

  16. Mycobacterium sarraceniae sp. nov. and Mycobacterium helvum sp. nov., isolated from the pitcher plant Sarracenia purpurea.

    Science.gov (United States)

    Tran, Phuong M; Dahl, John L

    2016-11-01

    Several fast- to intermediate-growing, acid-fast, scotochromogenic bacteria were isolated from Sarracenia purpurea pitcher waters in Minnesota sphagnum peat bogs. Two strains (DL734T and DL739T) were among these isolates. On the basis of 16S rRNA gene sequences, the phylogenetic positions of both strains is in the genus Mycobacterium with no obvious relation to any characterized type strains of mycobacteria. Phenotypic characterization revealed that neither strain was similar to the type strains of known species of the genus Mycobacterium in the collective properties of growth, pigmentation or fatty acid composition. Strain DL734T grew at temperatures between 28 and 32 °C, was positive for 3-day arylsulfatase production, and was negative for Tween 80 hydrolysis, urease and nitrate reduction. Strain DL739T grew at temperatures between 28 and 37 °C, and was positive for Tween 80 hydrolysis, urea, nitrate reduction and 3-day arylsulfatase production. Both strains were catalase-negative while only DL739T grew with 5 % NaCl. Fatty acid methyl ester profiles were unique for each strain. DL739T showed an ability to survive at 8 °C with little to no cellular replication and is thus considered to be psychrotolerant. Therefore, strains DL734T and DL739T represent two novel species of the genus Mycobacterium with the proposed names Mycobacterium sarraceniae sp. nov. and Mycobacterium helvum sp. nov., respectively. The type strains are DL734T (=JCM 30395T=NCCB 100519T) and DL739T (=JCM 30396T=NCCB 100520T), respectively.

  17. Paenibacillus oenotherae sp. nov. and Paenibacillus hemerocallicola sp. nov., isolated from the roots of herbaceous plants.

    Science.gov (United States)

    Kim, Tae-Su; Han, Ji-Hye; Joung, Yochan; Kim, Seung Bum

    2015-08-01

    Two Gram-staining-positive, aerobic, endospore-forming, motile bacteria, strains DT7-4T and DLE-12T, were isolated from roots of evening primrose (Oenothera biennis) and day lily (Hemerocallis fulva), respectively, and subjected to taxonomic characterization. Analysis of 16S rRNA gene sequences indicated that the two strains fell into two distinct phylogenetic clusters belonging to the genus Paenibacillus. Strain DT7-4T was most closely related to Paenibacillus phyllosphaerae PALXIL04T and Paenibacillus taihuensis THMBG22T, with 96.3% 16S rRNA gene sequence similarity to each, and strain DLE-12T was most closely related to Paenibacillus ginsengarvi Gsoil 139T and Paenibacillus hodogayensis SGT, with 96.6 and 93.3% sequence similarity, respectively. Both isolates contained anteiso-C15 : 0 as the dominant fatty acid, meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan and MK-7 as the respiratory menaquinone. The cellular polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and unidentified polar lipids. The DNA G+C contents of strains DT7-4T and DLE-12T were 50.1 ± 0.7 and 55.2 ± 0.5 mol%, respectively. The chemotaxonomic properties of both isolates were typical of members of the genus Paenibacillus. However, our biochemical and phylogenetic analyses distinguished each isolate from related species. Based on our polyphasic taxonomic analysis, strains DT7-4T and DLE-12T should be recognized as representatives of novel species of Paenibacillus, for which the names Paenibacillus oenotherae sp. nov. (type strain DT7-4T = KCTC 33186T = JCM 19573T) and Paenibacillus hemerocallicola sp. nov. (type strain DLE-12T = KCTC 33185T = JCM 19572T) are proposed.

  18. Surfactant protein A (SP-A)-mediated clearance of Staphylococcus aureus involves binding of SP-A to the staphylococcal adhesin eap and the macrophage receptors SP-A receptor 210 and scavenger receptor class A.

    Science.gov (United States)

    Sever-Chroneos, Zvjezdana; Krupa, Agnieszka; Davis, Jeremy; Hasan, Misbah; Yang, Ching-Hui; Szeliga, Jacek; Herrmann, Mathias; Hussain, Muzafar; Geisbrecht, Brian V; Kobzik, Lester; Chroneos, Zissis C

    2011-02-11

    Staphylococcus aureus causes life-threatening pneumonia in hospitals and deadly superinfection during viral influenza. The current study investigated the role of surfactant protein A (SP-A) in opsonization and clearance of S. aureus. Previous studies showed that SP-A mediates phagocytosis via the SP-A receptor 210 (SP-R210). Here, we show that SP-R210 mediates binding and control of SP-A-opsonized S. aureus by macrophages. We determined that SP-A binds S. aureus through the extracellular adhesin Eap. Consequently, SP-A enhanced macrophage uptake of Eap-expressing (Eap(+)) but not Eap-deficient (Eap(-)) S. aureus. In a reciprocal fashion, SP-A failed to enhance uptake of Eap(+) S. aureus in peritoneal Raw264.7 macrophages with a dominant negative mutation (SP-R210(DN)) blocking surface expression of SP-R210. Accordingly, WT mice cleared infection with Eap(+) but succumbed to sublethal infection with Eap- S. aureus. However, SP-R210(DN) cells compensated by increasing non-opsonic phagocytosis of Eap(+) S. aureus via the scavenger receptor scavenger receptor class A (SR-A), while non-opsonic uptake of Eap(-) S. aureus was impaired. Macrophages express two isoforms: SP-R210(L) and SP-R210(S). The results show that WT alveolar macrophages are distinguished by expression of SP-R210(L), whereas SR-A(-/-) alveolar macrophages are deficient in SP-R210(L) expressing only SP-R210(S). Accordingly, SR-A(-/-) mice were highly susceptible to both Eap(+) and Eap(-) S. aureus. The lungs of susceptible mice generated abnormal inflammatory responses that were associated with impaired killing and persistence of S. aureus infection in the lung. In conclusion, alveolar macrophage SP-R210(L) mediates recognition and killing of SP-A-opsonized S. aureus in vivo, coordinating inflammatory responses and resolution of S. aureus pneumonia through interaction with SR-A.

  19. Blastocystis sp. and other intestinal parasites in hemodialysis patients.

    Science.gov (United States)

    Kulik, Rose Anne; Falavigna, Dina Lúcia Morais; Nishi, Letícia; Araujo, Silvana Marques

    2008-08-01

    Chronic renal insufficiency disease (CRI) leads to uremia in hemodialysis patients and induces a state of immunodepression that results in higher frequencies of infections and diarrhea. Hemodialysis patients resident in the city of Campo Mourão, Paraná, Brazil were analyzed from April 2006 through September 2007 for Blastocystis sp. and other intestinal parasites and for associated diarrhea. Fecal samples from 86 hemodialysis patients and 146 healthy (reference) persons were examined by standard methods for detecting ova, larvae and cysts, which included preservation in 10% formalin and the Kinyoun method. Thirty-three hemodialysis patients (45.1%) and 36 reference individuals (25.7%) were found to be parasitized. The differences in the percentages of parasitism and polyparasitism between the reference group and the chronic renal patients was significant (p= 0.0318 and 0.0019, respectively). Blastocystis sp. (18%-20.1%), Endolimax nana (14%-16.3%), Cryptosporidium sp. (4%-4.7%) and Entamoeba coli (4%-4.7%) were the most frequent protozoa found in the hemodialysis patients. Parasitism was not significantly associated with diarrhea (p=0.9947) or with decreased white blood cell counts (p=0.7046) in these individuals. Because parasitic infections may be an important comorbidity factor in hemodialysis patients, we suggest that parasitological stool examinations, especially for Blastocystis sp. and Cryptosporidium sp., be included in routine medical follow-up examinations of these patients.

  20. Blastocystis sp. and other intestinal parasites in hemodialysis patients

    Directory of Open Access Journals (Sweden)

    Rose Anne Kulik

    Full Text Available Chronic renal insufficiency disease (CRI leads to uremia in hemodialysis patients and induces a state of immunodepression that results in higher frequencies of infections and diarrhea. Hemodialysis patients resident in the city of Campo Mourão, Paraná, Brazil were analyzed from April 2006 through September 2007 for Blastocystis sp. and other intestinal parasites and for associated diarrhea. Fecal samples from 86 hemodialysis patients and 146 healthy (reference persons were examined by standard methods for detecting ova, larvae and cysts, which included preservation in 10% formalin and the Kinyoun method. Thirty-three hemodialysis patients (45.1% and 36 reference individuals (25.7% were found to be parasitized. The differences in the percentages of parasitism and polyparasitism between the reference group and the chronic renal patients was significant (p= 0.0318 and 0.0019, respectively. Blastocystis sp. (18%-20.1%, Endolimax nana (14%-16.3%, Cryptosporidium sp. (4%-4.7% and Entamoeba coli (4%-4.7% were the most frequent protozoa found in the hemodialysis patients. Parasitism was not significantly associated with diarrhea (p=0.9947 or with decreased white blood cell counts (p=0.7046 in these individuals. Because parasitic infections may be an important comorbidity factor in hemodialysis patients, we suggest that parasitological stool examinations, especially for Blastocystis sp. and Cryptosporidium sp., be included in routine medical follow-up examinations of these patients.

  1. Production of *sp67*Ga at the Oslo Cyclotron

    International Nuclear Information System (INIS)

    Bjoernstad, T.; Holtebekk, T.

    1983-01-01

    A method for production of *sp67*Ga at the Oslo Cyclotron is described. The method is based on the nuclear reaction *sp68*Zn (p,2n)*sp67*Ga. The target is natural zinc metal of thickness 1.3 mm fixed by a thin alloy layer to a copper disc for efficient cooling during irradiation. By applying a beam of 29 MeV protons, a maximum production yield of approx. 1.8 mCi/*my*Ah was obtained. By demanding a contamination level of *sp66*Ga <=1%, the ''useful'' yield after a decaytime of 88 h is approx. 0.8 mCi/*my*Ah. Gallium has been separated carrierfree from the zinc matrix by cation exchange from 7.5M hydrocloric acid solutions and prepared as citrate complex at pH 5.5. After sterile filtering, autoclavation, pyrogene testing and analysis for iron and zinc, the *sp67*Ga-radiopharmaceutical has been applied in human investigations at the Ullevaal hospital in Oslo. (Auth.)

  2. The SP Theory of Intelligence: Benefits and Applications

    Directory of Open Access Journals (Sweden)

    J. Gerard Wolff

    2013-12-01

    Full Text Available This article describes existing and expected benefits of the SP theory ofintelligence, and some potential applications. The theory aims to simplify and integrate ideasacross artificial intelligence, mainstream computing, and human perception and cognition,with information compression as a unifying theme. It combines conceptual simplicitywith descriptive and explanatory power across several areas of computing and cognition.In the SP machine—an expression of the SP theory which is currently realized in theform of a computer model—there is potential for an overall simplification of computingsystems, including software. The SP theory promises deeper insights and better solutions inseveral areas of application including, most notably, unsupervised learning, natural languageprocessing, autonomous robots, computer vision, intelligent databases, software engineering,information compression, medical diagnosis and big data. There is also potential inareas such as the semantic web, bioinformatics, structuring of documents, the detection ofcomputer viruses, data fusion, new kinds of computer, and the development of scientifictheories. The theory promises seamless integration of structures and functions within andbetween different areas of application. The potential value, worldwide, of these benefits andapplications is at least $190 billion each year. Further development would be facilitatedby the creation of a high-parallel, open-source version of the SP machine, available toresearchers everywhere.

  3. Evolution of space food in Nostoc sp. HK-01

    Science.gov (United States)

    Tomita-Yokotani, Kaori; Yamashita, Masamichi; Hashimoto, Hirofumi; Sato, Seigo; Kimura, Yasuko; Katoh, Hiroshi; Arai, Mayumi

    2012-07-01

    Habitation in outer space is one of our challenges. We have been studying future space agriculture to provide food and oxygen for the habitation area in the space environment, on Mars. A cyanobacteria, Nostoc sp. HK-01, has high several outer space environmental tolerance. We have already confirmed that Nostoc sp.HK-01 had an ability to grow for over several years on the Martian regolith simulant in a laboratory experiment. Nostoc sp HK-01 would have high contribution to change the atmosphere in Mars as a photosynthetic creature. In outer environment, all of materials have to circulate for all of creature living in artificial eco-systems on Mars. This material has several functions as the utilization in space agriculture. Here, we are proposing using them as a food after its growing on Mars. We are trying to determine the best conditions and evolution for space food using Nostoc sp.HK-01 and studying the proposal of utilization of cyanobacteria, Nostoc sp HK-01, for the variation of meal as space agriculture.

  4. Albizia lebbeck, Leucaena leucocephala, Morinda lucida and Senna si

    African Journals Online (AJOL)

    SARAH

    2017-09-30

    Sep 30, 2017 ... Université des Sciences et Techniques de Masuku .... corresponded to the beginning of the dry season, while ... Statistical analysis: For each foliage data of nutrient .... Arbouche Y, Arbouche HS, Arbouche F, Arbouche R,.

  5. Performance of rabbits fed Leucaena leucocephala and concentrate ...

    African Journals Online (AJOL)

    Four fryers which were fed the same % of concentrates and Aspilia africana served as the control. Growth rate decreased at the beginning of the study followed by satisfactory growth thereafter. The control animals had a higher growth rate compared to the other two groups (P< 0.05). Alopecia and decreased appetite were ...

  6. Molecular prevalence of Bartonella, Babesia, and hemotropic Mycoplasma sp. in dogs with splenic disease.

    Science.gov (United States)

    Varanat, M; Maggi, R G; Linder, K E; Breitschwerdt, E B

    2011-01-01

    Among diseases that cause splenomegaly in dogs, lymphoid nodular hyperplasia (LNH), splenic hemangiosarcoma (HSA), and fibrohistiocytic nodules (FHN) are common diagnoses. The spleen plays an important role in the immunologic control or elimination of vector-transmitted, blood-borne pathogens, including Bartonella sp., Babesia sp., and hemotropic Mycoplasma sp. To compare the prevalence of Bartonella sp., Babesia sp., and hemotropic Mycoplasma sp. DNA in spleens from dogs with LNH, HSA, and FHN. Paraffin-embedded, surgically obtained biopsy tissues from LNH (N = 50), HSA (N = 50), and FHN (N = 37) were collected from the anatomic pathology archives. Spleens from specific pathogen-free (SPF) dogs (N = 8) were used as controls. Bartonella sp., Babesia sp., and Mycoplasma sp. DNA was amplified by PCR, followed by DNA sequencing. Bartonella sp. DNA was more prevalent in FHN (29.7%) and HSA (26%) as compared to LNH (10%) (P = .019, .0373, respectively) or control spleens (0.0%). The prevalence of Babesia sp. and hemotropic Mycoplasma sp. DNA was significantly lower than Bartonella sp. DNA in HSA (P = .0005, .006, respectively) and FHN (P = .003, .0004, respectively). There was no statistically significant difference in DNA prevalence among the 3 genera in the LNH group. The higher prevalence of Bartonella sp. in FHN and HSA warrants future investigations to determine if this bacterium plays a role in the development of these splenic diseases. Copyright © 2011 by the American College of Veterinary Internal Medicine.

  7. Vitreoscilla hemoglobin promotes Salecan production by Agrobacterium sp. ZX09.

    Science.gov (United States)

    Chen, Yun-mei; Xu, Hai-yang; Wang, Yang; Zhang, Jian-fa; Wang, Shi-ming

    2014-11-01

    Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. ZX09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan a promising material for applications in coagulation, lubrication, protection against acute liver injury, and alleviating constipation. In this study, we cloned the Vitreoscilla hemoglobin gene into a broad-host-range plasmid pCM158. Without antibiotic selection, there was negligible loss of the plasmid in the host Agrobacterium sp. ZX09 after one passage of cultivation. The expression of Vitreoscilla hemoglobin was demonstrated by carbon monoxide (CO) difference spectrum. The engineered strain Agrobacterium sp. ZX09 increased Salecan yield by 30%. The other physiological changes included its elevated respiration rate and cellular invertase activity.

  8. Biodegradation of 2-nitrotoluene by Micrococcus sp. strain SMN-1.

    Science.gov (United States)

    Mulla, Sikandar I; Hoskeri, Robertcyril S; Shouche, Yogesh S; Ninnekar, Harichandra Z

    2011-02-01

    A bacterial consortium capable of degrading nitroaromatic compounds was isolated from pesticide-contaminated soil samples by selective enrichment on 2-nitrotoluene as a sole source of carbon and energy. The three different bacterial isolates obtained from bacterial consortium were identified as Bacillus sp. (A and C), Bacillus flexus (B) and Micrococcus sp. (D) on the basis of their morphological and biochemical characteristics and by phylogenetic analysis based on 16S rRNA gene sequences. The pathway for the degradation of 2-nitrotoluene by Micrococcus sp. strain SMN-1 was elucidated by the isolation and identification of metabolites, growth and enzymatic studies. The organism degraded 2-nitrotoluene through 3-methylcatechol by a meta-cleavage pathway, with release of nitrite.

  9. Thawing of lithium in the SP-100 reactor core configuration

    International Nuclear Information System (INIS)

    Magee, P.M.; Malovrh, J.W.; REineking, W.H.

    1986-01-01

    The General Electric SP-100 Liquid Metal Reactor is designed to be launched with the lithium coolant in the reactor and primary loops frozen. Initial startup of the system in space, after a satisfactory orbit is achieved, will be accomplished by slowly increasing the power in the reactor core and using the heat generated to melt the lithium, first in the reactor, and then progressively down the primary loops. This technique significantly facilitates ground handling, reduces vibrational loads during vehicle launch and minimized the shuttle bay heat load. The challenge is to thaw the coolant and startup the system within an acceptable time without structural damage. The test results clearly demonstrate that thawing of the lithium in the SP-100 reactor core can be done rapidly without structural damage and, thus, support the selected concept of SP-100 launch with frozen lithium and thaw/startup in space

  10. Biochemical changes induced by fungicides in nitrogen fixing Nostoc sp.

    Science.gov (United States)

    Deviram, G V N S; Pant, Gaurav; Prasuna, R Gyana

    2013-01-01

    The present study indicates the effect of fungicides (approved by WHO) and their behavior on nitrogen fixer of rice eco system Nostoc sp. Application of plant protecting chemicals at recommended levels braced up the growth of blue green algae thereby enhancing heterocyst formation and nitrogenase activity. Nostoc sp demoed varying degrees of sensitivity to fungicides. Biomass yield, protein, carbohydrate content reduced after 3pg/mL concentration. Heterocyst damage was observed from 4μg/mL, Proline content increased with increase in fungicide concentration, utmost yellowing of the culture started from 4μg/mL. The decreasing order of the toxicity to Nostoc sp with fungicides was Mancozeb> Ediphenphos> Carbendazim> Hexaconazole.

  11. Uji produksi biosurfaktan oleh Pseudomonas sp. pada substrat yang berbeda

    Directory of Open Access Journals (Sweden)

    Fatimah Fatimah

    2012-02-01

    Full Text Available Biosurfactant, microbial metabolite whose properties like surfactant, was suggested to replace chemically synthesized surfactant for take in hand environtmental pollution by petroleum hydrocarbon. This work was done to examine potency of Pseudomonas sp. isolated from Tanjung Perak Harbor to produce biosurfactant. Also, to know the effect of different substrates (glucose + yeast extract, lubricating oil and hexadecane toward biosurfactant production. Pseudomonas sp. grown in mineral synthetic water and biosurfactant production was measured on stationary phase. Biosurfactant production based on emulsification activity and surface tension reduction of supernatant (using Du Nouy tensiometer. Solar, lubricating oil, and hexadecane were used to examine emulsification activity. Results indicated that Pseudomonas sp. have a potency to produce biosurfactant. Surface tension of supernatant decreased up to 20 dyne/cm, when grown on hexadecane substrate. Hexadecane is the best growing substrate for biosurfactant production than others.

  12. Ground testing of an SP-100 prototypic reactor

    International Nuclear Information System (INIS)

    Motwani, K.; Pflasterer, G.R.; Upton, H.; Lazarus, J.D.; Gluck, R.

    1988-01-01

    SP-100 is a space power system which is being developed by GE to meet future space electrical power requirements. The ground testing of an SP-100 prototypic reactor system will be conducted at the Westinghouse Hanford Company site located at Richland, Washington. The objective of this test is to demonstrate the performance of a full scale prototypic reactor system, including the reactor, control system and flight shield. The ground test system is designed to simulate the flight operating conditions while meeting all the necessary nuclear safety requirements in a gravity environment. The goal of the reactor ground test system is to establish confidence in the design maturity of the SP-100 space reactor power system and resolve the technical issues necessary for the development of a flight mission design

  13. Micetoma pulmonar por Scedosporium sp, reporte de dos casos

    Directory of Open Access Journals (Sweden)

    José G. Somocurcio

    2009-07-01

    Full Text Available Se reporta los dos primeros casos de micetoma pulmonar por Scedosporium sp, en el Perú, tratados quirúrgicamente en el Hospital Nacional Hipólito Unanue. Se practicó resección pulmonar debido a micetoma pulmonar de donde se tomó muestras que fueron enviadas a microbiología y anatomía patológica para cultivo y estudio histopatológico. Se identificó el moho Scedosporium sp en dos pacientes con secuelas cavitarias por tuberculosis, quienes presentaron tos y hemoptisis de dos meses y tres años de evolución, respectivamente. Radiológicamente las cavidades estaban ocupadas por una "bola fúngica". La histopatología indicó presencia de abundantes hifas, indistinguibles de las de Aspergillus sp, mientras que la inmunodifusión para Aspergillus fue negativa.

  14. Effectivity of the Earthworms Pheretima hupiensis, Eudrellus sp. and Lumbricus sp. on the Organic Matter Decomposition Process

    Directory of Open Access Journals (Sweden)

    Ea Kosman Anwar

    2009-05-01

    Full Text Available The earthworms are the one of soil fauna component in soil ecosystem have an important role in organic matter decomposition procces. The earthworm feed plant leaf and plant matter up to apart and dissolved. Earthworm metabolisms produce like faeces that mixed with decomposed organic matter mean vermicompost. The vermicompost fertility varies because of some kind of earthworm differ in “niche” and attitude. The experiment was to study the effectivity of earthworm on organic matter decomposition which has been conducted in Soil Biological and Healthy Laboratory and Green House of Soil Research Institute Bogor, during 2006 Budget Year. The three kind of earthworms i.e Pheretima hupiensis, Lumbricus sp. and Eudrellus sp. combined with three kind of organic matter sources i.e rice straw, trash and palm oil plant waste (compost heap. The result shows that the Lumbricus sp. are the most effective decomposer compared to Pheretima hupiensis and Eudrellus sp. and the organic matter decomposed by Lumbricus sp. as followed: market waste was decomposed of 100%, palm oil empty fruit bunch (compost heap 95.8 % and rice straw 84.9%, respectively. Earthworm effectively decreased Fe, Al, Mn, Cu dan Zn.

  15. Diversity and antimicrobial susceptibility of oxytetracycline-resistant isolates of Stenotrophomonas sp. and Serratia sp. associated with Costa Rican crops.

    Science.gov (United States)

    Rodríguez, C; Wachlin, A; Altendorf, K; García, F; Lipski, A

    2007-12-01

    To ameliorate the identification, evaluate the diversity, and determine the antimicrobial sensitivity of 19 oxytetracycline-resistant isolates of Stenotrophomonas sp. and Serratia sp. associated with Costa Rican crops. Phenotypical, chemotaxonomical, and molecular data allocated most isolates to the species Sten. maltophilia and Ser. marcescens. The API profiles, antimicrobial resistance patterns (ATB system), and BOX-polymerase chain reaction (PCR) genomic fingerprints of isolates of Stenotrophomonas sp. exhibited a higher degree of heterogeneity than those obtained for the isolates of Serratia sp. The former group of bacteria exhibited multiresistance to antimicrobials. In contrast, isolates of Serratia sp. were sensitive to the majority of the drugs tested. Changes in the results of the antibiograms throughout incubation, which indicate an induction of tolerance, were observed for isolates of both the species. Minimum inhibitory concentration of oxytetracycline, determined using E-test stripes, were rather elevated. The occurrence of two species of opportunistic pathogens in crop-associated materials poses a risk to consumers in the community. The phenotypic and genotypic data presented could support epidemiologist and physicians dealing with infections caused by environmental strains of these taxa.

  16. UJI KUALITATIF DAN KUANTITATIF EKSTRAK Sargassum sp. DAN Gracilaria sp. SEBAGAI INHIBITOR BIO-KOROSI PADA BAJA KARBON

    Directory of Open Access Journals (Sweden)

    Isriyanti Affifah

    2016-07-01

    Full Text Available Korosi atau perkaratan logam merupakan proses oksidasi suatu logam dengan udara atau elektrolit. Udara atau elektrolit tersebut akan mengalami reduksi, sehingga proses korosi merupakan proses elektrokimia. Pada penelitian sebelumnya diketahui bahwa korosi yang disebabkan mikroorganisme pengoksidasi besi (Thiobacillus ferooxidans memiliki peranan yang cukup signifikan terhadap kerugian ekonomi bagi industri. Lapisan biofilm yang dihasilkan mikroorganisme pada permukaan logam dapat mengubah karakteristik elektrokimia permukaan logam tersebut dan dapat menginduksi terjadinya korosi. Untuk mengatasi masalah tersebut, pada penelitian ini dilakukan ekstraksi Sargassum sp. dan Gracilaria sp. yang diduga efektif menginhibisi pertumbuhan mikroba pengoksidasi besi (Thibacillus ferooxidans yang biasanya terdapat di bangunan bawah laut. Hasil ekstraksi Sargassum sp. dan Gracilaria sp. menggunakan pelarut metanol-kloroform (1:1 memberikan yield terhadap berat basah sebesar 44,5% dan 36,5%. Ekstrak tersebut diuji bioaktivitasnya terhadap pertumbuhan T. ferooxidans secara kualitatif (kasat mata dan kuantitatif (metode weight-loss. Melalui kurva pertumbuhan diketahui bahwa T. ferooxidans mampu tumbuh sampai hari ke-7 dan mengalami fasa stasioner pada hari ke-8. Analisis metode weight-loss dilakukan menggunakan coupon dengan luas permukaan 3,6 cm2. Hasil analisis menunjukkan bahwa ekstrak Gracilaria sp mampu menginhibisi 29,3% lebih efektif daripada biocide komersial.

  17. The Role of S P2, SP3 AND SP4 in The Transcriptional Regulation of The Promoter of Nuclear Encoded Mitochondrial Genes

    International Nuclear Information System (INIS)

    Zaid, A.; Salem, Gh.

    2012-01-01

    The GC-box is an important transcriptional regulatory element present in the promoters of many mammalian genes, and is found in most, if not all, oxidative phosphorylation (OXPHOS) promoters. In the present study we examine the effects of three Spl family members (Sp2, Sp3, and Sp4) on the adenine nucleotide translocase 2, cytochrome cl, Fl-ATPase β-subunit, and the mitochondria transcription factor (mtTFA) promoters in Drosophila SL2 cell line. Sp3, like Spl, strongly activates transcription all four promoters. SP4 stimulates, moderately, but Sp2 had no effect. In addition, Sp3 can, like Spl, inhibit transcription from the proximal promoter of the ANT2 gene through binding to the Cbox GC element. By contrast, Sp4 and Sp2 do not repress promoter activity. Furthermore, since Sp4 and Sp2 bind to the Cbox repression element on the ANT2 promoter, but do not repress transcription, inhibition of transcription cannot be explained by steric hindrance of pre-initiation complex assembly. These data suggest that different Spl family members differentially affect transcription from the OXPHOS promoters.

  18. Ozone Technology for Pathogenic Bacteria of Shrimp (Vibrio sp.) Disinfection

    Science.gov (United States)

    Wulansarie, Ria; Dyah Pita Rengga, Wara; Rustamadji

    2018-03-01

    One of important marine commodities in Indonesia, shrimps are susceptible with Vibrio sp bacteria infection. That infection must be cleared. One of the technologies for disinfecting Vibrio sp. is ozone technology. In this research, Vibrio sp. is a pathogenic bacterium which infects Penaeus vannamei. Ozone technology is applied for threatening Vibrio sp. In this research, ozonation was performed in different pH. Those are neutral, acid (pH=4), and base (pH=9). The sample was water from shrimp embankment from Balai Besar Perikanan Budidaya Air Payau (BBPBAP) located in Jepara. That water was the habitat of Penaeus vannamei shrimp. The brand of ozonator used in this research was “AQUATIC”. The used ozonator in this research had 0,0325 g/hour concentration. The flow rate of sample used in this research was 2 L/minute. The ozonation process was performed in continuous system. A tank, pipe, pump, which was connected with microfilter, flowmeter and ozone generator were the main tools in this research. It used flowmeter and valve to set the flow rate scalable as desired. The first step was the insert of 5 L sample into the receptacle. Then, by using a pump, a sample supplied to the microfilter to be filtered and passed into the flow meter. The flow rate was set to 2 LPM. Furthermore, gas from ozonator passed to the flow for the disinfection of bacteria and then was recycled to the tank and the process run continuously. Samples of the results of ozonation were taken periodically from time 0, 3, 7, 12, 18, 24 to 30 minutes. The samples of the research were analyzed using Total Plate Count (TPC) test in BBPBAP Jepara to determine the number of Vibrio sp. bacteria. The result of this research was the optimal condition for pathogenic bacteria of shrimp (Vibrio sp.) ozonation was in neutral condition.

  19. Microfibril-associated Protein 4 Binds to Surfactant Protein A (SP-A) and Colocalizes with SP-A in the Extracellular Matrix of the Lung

    DEFF Research Database (Denmark)

    Schlosser, Anders; Thomsen, Theresa H.; Shipley, J. Michael

    2006-01-01

    for phagocytes. Here we describe the molecular interaction between the extracellular matrix protein microfibril-associated protein 4 (MFAP4) and SP-A. MFAP4 is a collagen-binding molecule containing a C-terminal fibrinogen-like domain and a N-terminal located integrin-binding motif. We produced recombinant MFAP4......-A composed of the neck region and carbohydrate recognition domain of SP-A indicating that the interaction between MFAP4 and SP-A is mediated via the collagen domain of SP-A. Monoclonal antibodies directed against MFAP4 and SP-A were used for immunohistochemical analysis, which demonstrates that the two...... molecules colocalize both on the elastic fibres in the interalveolar septum and in elastic lamina of pulmonary arteries of chronically inflamed lung tissue. We conclude, that MFAP4 interacts with SP-A via the collagen region in vitro, and that MFAP4 and SP-A colocates in different lung compartments...

  20. Multimeric and trimeric subunit SP-D are interconvertible structures with distinct ligand interaction

    DEFF Research Database (Denmark)

    Sørensen, Grith Lykke; Hoegh, Silje V; Leth-Larsen, Rikke

    2009-01-01

    -D compared to Met11 SP-D. Multimerization has proven important for enhancement of microbial phagocytosis. In the present study defined multimeric forms of Met11Thr SP-D were isolated from human amniotic fluid. Implementation of ManNAc-affinity chromatography allowed high recovery of natural trimeric SP......-D multimers. Trimeric SP-D subunits also showed greater binding to endogenous lipoproteins: LDL, oxLDL, and HDL, than multimeric SP-D. In conclusion, purified trimeric and multimeric SP-D represent separate and only partly interconvertible molecular populations with distinct biochemical properties....

  1. Mycelium characterization of the Amauroderma sp collected from Royal Belum

    International Nuclear Information System (INIS)

    Hassan Hamdani Mutaat; Mat Rasol Awang; Fauzi Daud; Rosnani Rashid; Meswan Maskom; Arfah Sumeri

    2010-01-01

    Etnobotanical study carried on the native community in the Royal Belum Forest areas had identified many potentially valuable spices that could be further explored for its medicinal and health purposes. The mushrooms, Amauroderma sp. was one of the resources that had been screened and collected for further investigation. In this works, the mycelium characterization of the mushroom was carried out in order to develop suitable seed or spawn for cultivation. The Amauroderma sp. mycelium, cultured from the fruit body tissue was found to grow well on the PDA plate. While on the potato extract liquid medium the mushroom could grow and produce stalk like fruit body. (author)

  2. SpF: Enabling Petascale Performance for Pseudospectral Dynamo Models

    Science.gov (United States)

    Jiang, W.; Clune, T.; Vriesema, J.; Gutmann, G.

    2013-12-01

    Pseudospectral (PS) methods possess a number of characteristics (e.g., efficiency, accuracy, natural boundary conditions) that are extremely desirable for dynamo models. Unfortunately, dynamo models based upon PS methods face a number of daunting challenges, which include exposing additional parallelism, leveraging hardware accelerators, exploiting hybrid parallelism, and improving the scalability of global memory transposes. Although these issues are a concern for most models, solutions for PS methods tend to require far more pervasive changes to underlying data and control structures. Further, improvements in performance in one model are difficult to transfer to other models, resulting in significant duplication of effort across the research community. We have developed an extensible software framework for pseudospectral methods called SpF that is intended to enable extreme scalability and optimal performance. High-level abstractions provided by SpF unburden applications of the responsibility of managing domain decomposition and load balance while reducing the changes in code required to adapt to new computing architectures. The key design concept in SpF is that each phase of the numerical calculation is partitioned into disjoint numerical 'kernels' that can be performed entirely in-processor. The granularity of domain-decomposition provided by SpF is only constrained by the data-locality requirements of these kernels. SpF builds on top of optimized vendor libraries for common numerical operations such as transforms, matrix solvers, etc., but can also be configured to use open source alternatives for portability. SpF includes several alternative schemes for global data redistribution and is expected to serve as an ideal testbed for further research into optimal approaches for different network architectures. In this presentation, we will describe the basic architecture of SpF as well as preliminary performance data and experience with adapting legacy dynamo codes

  3. Taenia sp. in human burial from Kan River, East Siberia.

    Science.gov (United States)

    Slepchenko, Sergey Mikhailovich; Ivanov, Sergey Nikolaevich; Vybornov, Anton Vasilevich; Alekseevich, Tsybankov Alexander; Sergeyevich, Slavinsky Vyacheslav; Lysenko, Danil Nikolaevich; Matveev, Vyacheslav Evgenievich

    2017-05-01

    We present an arhaeoparasitological analysis of a unique burial from the Neftprovod II burial ground in East Siberia, which dated from the Bronze Age. Analysis of a sediment sample from the sacral region of the pelvis revealed the presence of Taenia sp. eggs. Because uncooked animal tissue is the primary source of Taenia, this indicated that the individual was likely consuming raw or undercooked meat of roe deer, red deer, or elk infected with Taenia. This finding represents the oldest case of a human infected with Taenia sp. from Eastern Siberia and Russia.

  4. Aquatic Fern (Azolla Sp.) Assisted Synthesis of Gold Nanoparticles

    Science.gov (United States)

    Jha, Anal K.; Prasad, K.

    2016-02-01

    Aquatic pteridophyte (Azolla sp.) was taken to assess its potential to synthesize the metal (Au) nanoparticles. The synthesized particles were characterized using X-ray, UV-visible, scanning and transmission electron microscopy analyses. Nanoparticles almost spherical in shape having the sizes of 5-17nm are found. UV-visible study revealed the surface plasmon resonance at 538nm. Responsible phytochemicals for the transformation were principally phenolics, tannins, anthraquinone glycosides and sugars present abundantly in the plant thereby bestowing it adaptive prodigality. Also, the use of Azolla sp. for the synthesis of gold nanoparticles offers the benefit of eco-friendliness.

  5. Profil des examens radiographiques spéciaux conventionnels dans ...

    African Journals Online (AJOL)

    But : Montrer l'apport des examens spéciaux dans la démarche diagnostique Méthodologie : Il s'agit d'une étude rétrospective qui a porté sur les résultats de 249 examens spéciaux colligés dans le service de radiologie du CHU- Tokoin de Lomé du 1er mars 2003 au 1er mars 2004. Résultats : L'âge de nos patients varie ...

  6. Production of Cold Active Lipase from Bacillus sp.

    OpenAIRE

    Yasemin, Sara; Arabacı, Nihan; Korkmaz Güvenmez, Hatice

    2018-01-01

    A cold active lipase producing Bacillus sp. strains were isolated from sewage of oil. Bacillus sp. strain SY-7 was determined as the best lipase producing isolate. The highest enzyme production was found at 20°C and pH 8.0 on tributyrin media. Analyses of molecular mass of the partial purified lipase was carried out by SDS-PAGE which revealed a single band as 110.5 kDa. The enzyme activity and stability were determined by spectrophotometric and titrimetric methods. The enzyme was active betwe...

  7. A comparative study on phyllosphere nitrogen fixation by newly isolated Corynebacterium sp. & Flavobacterium sp. and their potentialities as biofertilizer.

    Science.gov (United States)

    Giri, S; Pati, B R

    2004-01-01

    A number of nitrogen fixing bacteria has been isolated from forest phyllosphere on the basis of nitrogenase activity. Among them two best isolates are selected and identified as Corynebacterium sp. AN1 & Flavobacterium sp. TK2 able to reduce 88 and 132 n mol of acetylene (10(8)cells(-1)h(-1)) respectively. They were grown in large amount and sprayed on the phyllosphere of maize plants as a substitute for nitrogenous fertilizer. Marked improvements in growth and total nitrogen content of the plant have been observed by the application of these nitrogen-fixing bacteria. An average 30-37% increase in yield was obtained, which is nearer to chemical fertilizer treatment. Comparatively better effect was obtained by application of Flavobacterium sp.

  8. Actinomadura amylolytica sp. nov. and Actinomadura cellulosilytica sp. nov., isolated from geothermally heated soil.

    Science.gov (United States)

    Jiao, Jian-Yu; Liu, Lan; Zhou, En-Min; Wei, Da-Qiao; Ming, Hong; Xian, Wen-Dong; Yuan, Chang-Guo; Zhong, Jing-Mei; Li, Wen-Jun

    2015-07-01

    Two aerobic, Gram-positive actinomycetes, designated YIM 77502(T) and YIM 77510(T), were isolated from geothermally heated soil of Tengchong county, Yunnan province, south-west China. The taxonomic position of strains YIM 77502(T) and YIM 77510(T) were investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains YIM 77502(T) and YIM 77510(T) belong to the genus Actinomadura. Both strains form extensively-branched substrate and aerial mycelia which differentiated into short spore chains. The cell wall of the two strains contained meso-diaminopimelic acid, while the whole-cell sugars detected were glucose, madurose, mannose and rhamnose. The polar lipid profile of strain YIM 77502(T) was found to consist of diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, two unidentified phospholipids and an unidentified polar lipid, while strain YIM 77510(T) consisted of diphosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. The respiratory quinones of strains YIM 77502(T) and YIM 77510(T) were MK-9(H6) and MK-9(H8). The major fatty acids (>10 %) of strain YIM 77502(T) were C17:0, iso-C16:0, C17:010-methyl and iso-C18:0, and those of strain YIM 77510(T) were iso-C16:0, C17:010-methyl and iso-C18:0. The G+C contents of strains YIM 77502(T) and YIM 77510(T) were determined to be 71.3 and 70.2 mol%, respectively. The DNA-DNA hybridization values of strains YIM 77502(T), YIM 77510(T) and their closest phylogenetic neighbours Actinomadura echinospora BCRC 12547(T) and Actinomadura umbrina KCTC 9343(T) were less than 70 %. Based on the morphological and physiological properties, and phylogenetic analyses, strains YIM 77502(T) and YIM 77510(T) are considered to represent two novel species of the genus Actinomadura, for which the names Actinomadura amylolytica sp. nov. (type strain YIM 77502(T) = DSM 45822(T) = CCTCC AA 2012024(T)) and Actinomadura cellulosilytica sp. nov. (type

  9. Enraizamiento in vitro de Dioscoreas sp. In vitro rootting of Dioscoreas sp.

    Directory of Open Access Journals (Sweden)

    Jarma Alfredo

    2003-12-01

    Full Text Available El presente estudio se realizó en el Laboratorio de Cultivo de Tejidos Vegetales de la Universidad de Córdoba en el año 2000 con el objetivo de evaluar el efecto del ácido naftalenacético (ANA en el medio de cultivo sobre el enraizamiento in vitro de tres cultivares de ñame (Dioscorea sp.. Se estudió el efecto de cuatro concentraciones del regulador de crecimiento (0, 0.3, 0.6 y 0.9 mg/1 sobre tres cultivares de ñame (Diamantes-22, 003 y 005. Se empleó un experimento trifactorial con diseño aleatorio y 20 repeticiones; cada unidad experimental estuvo conformada por un recipiente de vidrio que contenía el medio de cultivo y el explante (segmento nodal. Las variables consideradas fueron número y grosor de raíces, oxidación del medio de cultivo y producción de callo. Los resultados indicaron que tanto la hormona como el genotipo tuvieron efecto sobre todas las variables consi­deradas en el estudio y que la interacción fue importante (PThe Universidad de Córdoba s Vegetal Tissue Culture Laboratory evaluated the effect of naphthalenacetic acid (NAA on in vitro rooting of three yam cultivars (Dioscorea sp. in 2000. The effect of four hormone levels (0, 0.3, 0.6 and 0.9 mg/1 was studied on three yam cultivars (Diamantes-22,003 and 005. A random experimental design was used employing 4x3 factorial arrangement and 20 repetitions; each experimental unit consisted of a glass receptacle containing the culture medium and the explant (one segment nodal. The variables considered were the number of roots and their thickness, culture medium oxidation and callus production. Findings showed that both the hormone and genotype had an effect on all those variables considered in the study and interaction was significant (P <0.05 for callus production and culture medium oxidation. The 005 cultivar showed the greatest root number and thickness values. It was also determined that when a 0.6 and 0.9 mg/1 dose of auxin was applied, root production and

  10. Bacillus beijingensis sp. nov. and Bacillus ginsengi sp. nov., isolated from ginseng root.

    Science.gov (United States)

    Qiu, Fubin; Zhang, Xiaoxia; Liu, Lin; Sun, Lei; Schumann, Peter; Song, Wei

    2009-04-01

    Four alkaligenous, moderately halotolerant strains, designated ge09, ge10(T), ge14(T) and ge15, were isolated from the internal tissue of ginseng root and their taxonomic positions were investigated by using a polyphasic approach. Cells of the four strains were Gram-positive-staining, non-motile, short rods. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains ge09 and ge10(T) formed one cluster and strains ge14(T) and ge15 formed another separate cluster within the genus Bacillus. 16S rRNA gene sequence similarities with type strains of other Bacillus species were less than 97 %. Levels of DNA-DNA relatedness among the four strains showed that strains ge09 and ge10(T) and strains ge14(T) and ge15 belonged to two separate species; the mean level of DNA-DNA relatedness between ge10(T) and ge14(T) was only 28.7 %. Their phenotypic and physiological properties supported the view that the two strains represent two different novel species of the genus Bacillus. The DNA G+C contents of strains ge10(T) and ge14(T) were 49.9 and 49.6 mol%, respectively. Strains ge10(T) and ge14(T) showed the peptidoglycan type A4alpha l-Lys-d-Glu. The lipids present in strains ge10(T) and ge14(T) were diphosphatidylglycerol, phosphatidylglycerol, a minor amount of phosphatidylcholine and two unknown phospholipids. Their predominant respiratory quinone was MK-7. The fatty acid profiles of the four novel strains contained large quantities of branched and saturated fatty acids. The predominant cellular fatty acids were iso-C(15 : 0) (42.5 %), anteiso-C(15 : 0) (22.2 %), anteiso-C(17 : 0) (7.3 %) and C(16 : 1)omega7c alcohol (5.7 %) in ge10(T) and iso-C(15 : 0) (50.7 %) and anteiso-C(15 : 0) (20.1 %) in ge14(T). On the basis of their phenotypic properties and phylogenetic distinctiveness, two novel species of the genus Bacillus are proposed, Bacillus beijingensis sp. nov. (type strain ge10(T) =DSM 19037(T) =CGMCC 1.6762(T)) and Bacillus ginsengi sp. nov. (type strain ge14

  11. Possible linkage of SP6 transcriptional activity with amelogenesis by protein stabilization.

    Science.gov (United States)

    Utami, Trianna W; Miyoshi, Keiko; Hagita, Hiroko; Yanuaryska, Ryna Dwi; Horiguchi, Taigo; Noma, Takafumi

    2011-01-01

    Ameloblasts produce enamel matrix proteins such as amelogenin, ameloblastin, and amelotin during tooth development. The molecular mechanisms of ameloblast differentiation (amelogenesis) are currently not well understood. SP6 is a transcription factor of the Sp/KLF family that was recently found to regulate cell proliferation in a cell-type-specific manner. Sp6-deficient mice demonstrate characteristic tooth anomalies such as delayed eruption of the incisors and supernumerary teeth with disorganized amelogenesis. However, it remains unclear how Sp6 controls amelogenesis. In this study, we used SP6 high producer cells to identify SP6 target genes. Based on the observations that long-term culture of SP6 high producer cells reduced SP6 protein expression but not Sp6 mRNA expression, we found that SP6 is short lived and specifically degraded through a proteasome pathway. We established an in vitro inducible SP6 expression system coupled with siRNA knockdown and found a possible linkage between SP6 and amelogenesis through the regulation of amelotin and Rock1 gene expression by microarray analysis. Our findings suggest that the regulation of SP6 protein stability is one of the crucial steps in amelogenesis.

  12. Possible Linkage of SP6 Transcriptional Activity with Amelogenesis by Protein Stabilization

    Directory of Open Access Journals (Sweden)

    Trianna W. Utami

    2011-01-01

    Full Text Available Ameloblasts produce enamel matrix proteins such as amelogenin, ameloblastin, and amelotin during tooth development. The molecular mechanisms of ameloblast differentiation (amelogenesis are currently not well understood. SP6 is a transcription factor of the Sp/KLF family that was recently found to regulate cell proliferation in a cell-type-specific manner. Sp6-deficient mice demonstrate characteristic tooth anomalies such as delayed eruption of the incisors and supernumerary teeth with disorganized amelogenesis. However, it remains unclear how Sp6 controls amelogenesis. In this study, we used SP6 high producer cells to identify SP6 target genes. Based on the observations that long-term culture of SP6 high producer cells reduced SP6 protein expression but not Sp6 mRNA expression, we found that SP6 is short lived and specifically degraded through a proteasome pathway. We established an in vitro inducible SP6 expression system coupled with siRNA knockdown and found a possible linkage between SP6 and amelogenesis through the regulation of amelotin and Rock1 gene expression by microarray analysis. Our findings suggest that the regulation of SP6 protein stability is one of the crucial steps in amelogenesis.

  13. Shewanella hafniensis sp. nov. and Shewanella morhuae sp. nov., isolated from marine fish of the Baltic Sea

    DEFF Research Database (Denmark)

    Satomi, M.; Vogel, Birte Fonnesbech; Gram, Lone

    2006-01-01

    Two novel species belonging to the genus Shewanella are described on the basis of their phenotypic characteristics, phylogenetic analyses of 16S rRNA and gyrB gene sequences and levels of DNA-DNA hybridization. A total of 47 strains belonging to two novel Gram-negative, psychrotolerant, H2S-produ...... species, Shewanella hafniensis sp. nov. (type strain P010T=ATCC BAA-1207T=NBRC 100975T) and Shewanella morhuae sp. nov. (type strain U1417T=ATCC BAA-1205T=NBRC 100978T), are described....

  14. Ocorrência de Babesia sp em pequenos roedores no Brasil Occurrence of Babesia sp in small rodents in Brazil

    OpenAIRE

    G.S. Gazeta; R.W. Carvalho; R.F. Avelar; M. Amorim; A.E. Aboud-Dutra

    2004-01-01

    Foi analisada a ocorrência de babesiose em pequenos roedores nos municípios de Silva Jardim e Nova lguaçu, Estado do Rio de Janeiro. Foram capturados 44 roedores de seis espécies diferentes e entre eles a prevalência da infecção foi de 27,3%. Rattus norvegicus foi considerado o principal reservatório (50,0%) e Oligoryzomys nigripes como novo hospedeiro para Babesia sp. Este foi o primeiro relato de Babesia sp. em roedores no Brasil. A freqüência de roedores positivos e o risco de infecção dos...

  15. Heitmania gen. nov., a new yeast genus in Microbotryomycetes, and description of three novel species: Heitmania litseae sp. nov., Heitmania castanopsis sp. nov. and Heitmania elacocarpi sp. nov.

    Science.gov (United States)

    Liu, Xin-Zhan; Groenewald, Marizeth; Boekhout, Teun; Bai, Feng-Yan

    2017-11-01

    Nine anamorphic yeast strains isolated from various plant leaves collected in southern China were phylogenetically characterized based on sequences of the internal transcribed spacer (ITS) region, the D1/D2 domains of the large subunit (LSU) rRNA gene, the small subunit (SSU) rRNA gene, the two subunits of the RNA polymerase II gene (RPB1 and RPB2) and the translation elongation factor 1-α (TEF1). Phylogenetic analysis of the combined sequences of the six genes showed that the new strains formed a distinct clade in the class Microbotryomycetes but could not be assigned to any of the existing genera, families or orders of the class. Three separate groups were consistently resolved from the nine new strains based on the combined sequences of the six genes and single gene sequences of ITS, RPB1, RPB2 and TEF1. The results suggest that the nine yeast strains compared represent three novel species in a novel genus. The names Heitmania gen. nov. (MycoBank registration number MB819987), Heitmania litseae sp. nov. (MB820112, type strain CGMCC 2.5697 T =CBS 14756 T ), Heitmania castanopsis sp. nov. (MB819988, CGMCC 2.5698 T =CBS 14750 T ) and Heitmania elacocarpi sp. nov. (MB820113, CGMCC 2.5695 T =CBS 14752 T ) are proposed for the new taxa.

  16. Isolation of high-salinity-tolerant bacterial strains, Enterobacter sp., Serratia sp., Yersinia sp., for nitrification and aerobic denitrification under cyanogenic conditions.

    Science.gov (United States)

    Mpongwana, N; Ntwampe, S K O; Mekuto, L; Akinpelu, E A; Dyantyi, S; Mpentshu, Y

    2016-01-01

    Cyanides (CN(-)) and soluble salts could potentially inhibit biological processes in wastewater treatment plants (WWTPs), such as nitrification and denitrification. Cyanide in wastewater can alter metabolic functions of microbial populations in WWTPs, thus significantly inhibiting nitrifier and denitrifier metabolic processes, rendering the water treatment processes ineffective. In this study, bacterial isolates that are tolerant to high salinity conditions, which are capable of nitrification and aerobic denitrification under cyanogenic conditions, were isolated from a poultry slaughterhouse effluent. Three of the bacterial isolates were found to be able to oxidise NH(4)-N in the presence of 65.91 mg/L of free cyanide (CN(-)) under saline conditions, i.e. 4.5% (w/v) NaCl. The isolates I, H and G, were identified as Enterobacter sp., Yersinia sp. and Serratia sp., respectively. Results showed that 81% (I), 71% (G) and 75% (H) of 400 mg/L NH(4)-N was biodegraded (nitrification) within 72 h, with the rates of biodegradation being suitably described by first order reactions, with rate constants being: 4.19 h(-1) (I), 4.21 h(-1) (H) and 3.79 h(-1) (G), respectively, with correlation coefficients ranging between 0.82 and 0.89. Chemical oxygen demand (COD) removal rates were 38% (I), 42% (H) and 48% (G), over a period of 168 h with COD reduction being highest at near neutral pH.

  17. Habitat Perkembangbiakan Spesifik Anopheles sp Di Tambang Emas Kura-Kura Banian (Perubahan Perilaku Anopheles sp

    Directory of Open Access Journals (Sweden)

    Liestiana Indriyati, SKM

    2016-12-01

    Full Text Available The results of Health Research 2010, showed that malaria was an infectious disease that the sixth cause of death cause of the sixth of death in Indonesia. Kotabaru is a malaria endemic district, especially in Puskesmas Banian, Annual Parasite Incidence 241.19 in 2014 where the cases of malaria with occurring in illegal gold mines on the slopes of Banian Mountain. It’s needed to know the factors that affect the existence of malaria in these locations. This research was observational study doing by cross-sectional study and analyzed by descriptively. Research conducted at the Gold Mine Banian Buluh Kuning village, Sungai Durian subdistric, Kotabaru district, South Kalimantan Province in February and March, 2015. The activities were catching larva and adult mosquitoes, environmental and mosquito breeding habitats observation and Mass Blood Survey. It was found Anopheles maculatus and Anopheles leucosphyrus with very low densities in area with the number of malaria cases by 22.99%. It was found divers breeding habitats of Anopheles sp on a former gold wash, former drum and former plastic containers. This indicated a behavioral change of Anopheles breeding places because previous to this the Anopheles breed in puddles on the ground or direct contact with the ground.

  18. Increased constituent ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. and a decrease in microflora diversity may be indicators of ventilator-associated pneumonia: a prospective study in the respiratory tracts of neonates.

    Directory of Open Access Journals (Sweden)

    Wei Lu

    Full Text Available Ventilator-associated pneumonia (VAP is a common complication and cause of death in neonates on mechanical ventilation. However, it is difficult to define the causes of VAP. To understand the causes of VAP, we undertook a prospective study based on the diversity of the microflora in VAP. The experimental group consisted of newborns who suffered from respiratory distress syndrome (RDS and VAP, while the control group suffered from RDS without VAP. Sputa were collected within 1, 3, and 5 days of ventilation and were divided into six groups. DNA was extracted from the samples, and the 16S rDNA was PCR amplified, separated using denaturing gradient gel electrophoresis (DGGE, cloned and sequenced. The resulting sequences were compared using BLAST. The DGGE pictures were measured, and the richness, Shannon-Wiener index, and cluster maps were analyzed. No differences were found regarding the constituent ratio of any genus between the Non-VAP and VAP group within 1 day after intubation. After 1 to 3 days, the constituent ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. in the VAP group were higher than those in the Non-VAP group, and the ratios of Serratia sp. and Achromobacter sp. were lower. After 3 to 5 days, the ratios of Klebsiella sp., Acinetobacter sp., Serratia sp., and Achromobacter sp. were lower than those in the Non-VAP group. The richness and Shannon-Wiener index of the Non-VAP group were higher than those of the VAP group from 1 to 3 days after intubation, while no differences were found within 1 day and from 3 to 5 days. We conclude that during the first three days of intubation, the microflora diversity in the lower respiratory tract was reduced due to VAP, and the greater constituent ratios of Klebsiella sp., Acinetobacter sp., and Streptococcus sp. in the sputum may be indicators of VAP.

  19. Bioaccumulation of platinum group metals in dolphins, Stenella sp ...

    African Journals Online (AJOL)

    Platinum group metals (PGMs) concentrations were measured in the tissues= of dolphins (Stenella sp.) caught along the Ghanaian coastline. Tissues from specimens caught by fishermen from Dixcove, western Ghana, were analysed in 2006 for palladium (Pd), platinum (Pt) and rhodium (Rh) using the Neutron Activation ...

  20. Ascoaphaera osmophila sp.nov. An Australian Spore Cyst

    DEFF Research Database (Denmark)

    Skou, Jens-Peder; King, , J.

    1984-01-01

    Ascosphaera osmophila sp. nov. is described. Septa occur often close together and remain intact when the mycelium disintegrates. A fairly good production of mature spore cysts occurs only on media containing 10% sugar or more. A. osmophila lives in association with the mason bee, Chalicodoma...

  1. Comparative study of Aspergillus niger and Penicillium sp. in the ...

    African Journals Online (AJOL)

    STORAGESEVER

    2010-06-14

    Jun 14, 2010 ... The comparative study of Aspergillus niger and Penicillium sp. in the biodegradation of automotive gas oil (AGO) and premium motor spirit (PMS) was carried out to ascertain the effectiveness of using these microorganisms in cleaning and restoring the ecosystem when polluted by petroleum products.

  2. Expression of Heterologous Cellulases in Thermotoga sp. Strain RQ2

    Directory of Open Access Journals (Sweden)

    Hui Xu

    2015-01-01

    Full Text Available The ability of Thermotoga spp. to degrade cellulose is limited due to a lack of exoglucanases. To address this deficiency, cellulase genes Csac_1076 (celA and Csac_1078 (celB from Caldicellulosiruptor saccharolyticus were cloned into T. sp. strain RQ2 for heterologous overexpression. Coding regions of Csac_1076 and Csac_1078 were fused to the signal peptide of TM1840 (amyA and TM0070 (xynB, resulting in three chimeric enzymes, namely, TM1840-Csac_1078, TM0070-Csac_1078, and TM0070-Csac_1076, which were carried by Thermotoga-E. coli shuttle vectors pHX02, pHX04, and pHX07, respectively. All three recombinant enzymes were successfully expressed in E. coli DH5α and T. sp. strain RQ2, rendering the hosts with increased endo- and/or exoglucanase activities. In E. coli, the recombinant enzymes were mainly bound to the bacterial cells, whereas in T. sp. strain RQ2, about half of the enzyme activities were observed in the culture supernatants. However, the cellulase activities were lost in T. sp. strain RQ2 after three consecutive transfers. Nevertheless, this is the first time heterologous genes bigger than 1 kb (up to 5.3 kb in this study have ever been expressed in Thermotoga, demonstrating the feasibility of using engineered Thermotoga spp. for efficient cellulose utilization.

  3. Phellinus artemisiae sp. nov. (Basidiomycota, Hymenochaetaceae), from western USA

    Czech Academy of Sciences Publication Activity Database

    Vlasák, Josef; Vlasák, J.

    2017-01-01

    Roč. 303, č. 1 (2017), s. 93-96 ISSN 1179-3155 Institutional support: RVO:60077344 Keywords : chaparral Fungi * Phellinus artemisiae sp. nov. * molecular taxonomy * Fungi Subject RIV: EF - Botanics OBOR OECD: Plant sciences, botany Impact factor: 1.240, year: 2016

  4. Biological characterisation of Haliclona (?gellius) sp.: sponge and associated microorganisms.

    NARCIS (Netherlands)

    Sipkema, D.; Holmes, B.; Nichols, S.A.; Blanch, H.W.

    2009-01-01

    We have characterised the northern Pacific undescribed sponge Haliclona (?gellius) sp. based on rDNA of the sponge and its associated microorganisms. The sponge is closely related to Amphimedon queenslandica from the Great Barrier Reef as the near-complete 18S rDNA sequences of both sponges were

  5. Cytosporones O, P and Q from an endophytic Cytospora sp

    DEFF Research Database (Denmark)

    Abreu, L.M.; Phipps, Richard Kerry; Pfenning, L.H.

    2010-01-01

    Cytosporones O, P and Q, together with the known compounds cytosporones B, C, D, E and dothiorelones A, 13, C. and H were isolated from the ascomycete fungus Cytospora sp. during a chemotaxonomic study Of fungal endophytes belonging to the related genera Cytospora and Phomopsis from Brazil. The s...

  6. Beta-Glucan Synthase Gene Expression in Pleurotus sp

    International Nuclear Information System (INIS)

    Azhar Mohamad; Nie, H.J.

    2016-01-01

    Pleurotus sp. is a popular edible mushroom, containing various functional component, in particular, Beta-glucan. Beta-glucans is a part of glucan family of polysaccharides and supposedly contribute to medicinal and nutritional value of Pleurotus.sp. In order to understand the distribution of Beta-glucan in Pleurotus.sp, the Beta-glucan synthase gene expression was determined and compared in different part of Pleurotus, namely mycelium, stripe and cap. The Pleurotus.sp RNA was extracted using commercial kit, employing Tissuelyser ll (Qiagen, USA) to disrupt the cell walls. Then the RNA was quantified by Nano drop (Thermo Fisher, USA) and visualized using denaturing agarose gel. RNA with good OD 260.280 reading (∼2.0) was chosen and converted to cDNA. Using Laccase synthase gene as home keeping gene, Beta-glucan synthase gene expression was quantified using CFX 96 Real Time PCR detection system (Biorad, USA). Preliminary result shows that Beta-glucan synthase was relatively expressed the most in stripe, followed by mycelium and barely in cap. (author)

  7. Occurrence of Anatrichosoma sp. in a Nigerian feral cat | Ohiolei ...

    African Journals Online (AJOL)

    This is a report of the presence of an embryonated egg with distinct morphological features consistent with those of Anatrichosoma sp., in cat faeces from Ibadan, Nigeria. This is important because detailed information about this parasite's clinical pattern, epidemiology and host range is just being gradually unravelled.

  8. Reproductive status of opisthoteuthis sp . Over an annual cycle ...

    African Journals Online (AJOL)

    The reproductive biology of deepwater cephalopods has received relatively little attention. The cirrate octopus Opisthoteuthis sp. is a regular bycatch in deep benthic trawls from 800 to 1 200 m deep west of Scotland. Specimens were sampled throughout the seasonal cycle. Mature females occurred over a broad size range ...

  9. Polydactyly in the central pacific gecko, Lepidodactylus sp. (Squamata: Gekkonidae)

    Science.gov (United States)

    Bauer, A.M.; Hathaway, S.A.; Fisher, R.N.

    2009-01-01

    We report the first known case of naturally occurring polydactyly in a gekkotan lizard. A single individual from Palmyra Atoll exhibited a triplication of digit III of the m hand. No obvious teratogenic sources are present on the atoll and the causal factors of polydactyly in Lepidodactylus sp. remain unknown.

  10. Bioreduction of chromate by immobilized cells of Halomonas sp

    Energy Technology Data Exchange (ETDEWEB)

    Murugavelh, S.; Mohanty, Kaustubha [Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati – 781039, Assam (India)

    2013-07-01

    In this work, the bioreduction of Cr(VI) by immobilized cells of Halomonas sp was reported. Ca alginate, acryl amide and agar were tested as the matrices for immobilization. Ca alginate was found to be the suitable matrix among the different matrices studied. Of the various dosages of inoculum studied 2 g/L was found to be the optimum. Glucose at 1 g L-1 was completely utilized by the immobilized Halomonas sp even in the presence of Cr(VI) at 40 mg L-1. The optimum pH for the bioreduction of Cr(VI) by immobilized Halomonas sp was found to be pH 6. The mechanical strength of the beads plays an essential role in the bioreduction process. Halomonas sp entrapped in a alginate matrix reported a maximum of 98.9 % of reduction for an initial Cr(VI) concentration of 10 mg L-1. The alginate beads can be reused for 3 times with slight drop in the percentage reduction. The presence of other metals decreased the bioreduction percentage.

  11. Telosticta fugispinosa sp. nov. from Sabah (Odonata: Zygoptera: Platystictidae).

    Science.gov (United States)

    Dow, Rory A; Afendy, Aqilah; Rahman, Homathevi

    2016-04-14

    Telosticta fugispinosa sp. nov. (holotype male, from Borneo, Sabah, West Coast division, Crocker Range National Park, Inobong, Kimamabang waterfall stream system, 21 ix 2012, deposited in RMNH) is described from Kinabalu National Park and Crocker Range National Park in Sabah, Malaysian Borneo. It is distinguished from all other species of Telosticta by the form of the male anal appendages.

  12. Culture characteristics of Candida sp in waste conversion ...

    African Journals Online (AJOL)

    A strain of Candida sp. was isolated from ripe banana pulp during the preliminary phase of a process for the production of a protein-enriched feed supplement. Morphological and biochemical tests demonstrated that the strain, which was bipolar and elongated, was not capable of growth at 37ºC but grew at room ...

  13. THE SAGA OF A MALE FERTILITY PROTEIN (SP22)

    Science.gov (United States)

    Toxicologic studies designed to identify chemical-induced alterations in the structure and function of the epididymis, particularly the acquisition of fertility by proximal cauda epididymal sperm, have lead to the discovery of a novel sperm protein (SP22) that is well correlated ...

  14. Isozyme-based genetic fingerprinting of Manihot sp

    African Journals Online (AJOL)

    Prof. Ogunji

    1973-06-22

    Jun 22, 1973 ... Isozyme-based genetic fingerprinting of Manihot sp. Efisue, A. A.. Development of Crop & Soil Science, University of Port Harcourt P.M.B. 5323 Choba, Port Harcourt,. Rivers State, Nigeria. (Received 29:10:13, Accepted 20:12:13). Abstract. Many cassava varieties have been released into farmers' fields in ...

  15. Wolbachia Sebagai Alternatif Pengendalian Vektor Nyamuk Aedes SP.

    OpenAIRE

    Lusiyana, Novyan

    2014-01-01

    Demam Berdarah Dengue (DBD) adalah penyakit yang disebabkan oleh virus dengue. Virus dengue ditransmisikan kepada manusia melalui perantara vektor yaitu nyamuk Aedes aegypti dan Aedes albopictus (Aedes sp.). Penyakit ini dapat terjadi sepanjang tahun dan meningkat seiring dengan datangnya musim penghujan. Penyakit DBD memiliki mortalitas yang cukup tinggi terutama di negara-negara beriklim tropis dimana banyak faktor yang mendukung perkembangbiakan nyamuk vektor tersebut.

  16. Optimization of cholesterol oxidase production by Brevibacterium sp ...

    African Journals Online (AJOL)

    An ultrasound-assisted emulsification as a pretreatment for cholesterol oxidase production by submerge fermentation using Brevibacterium sp. in a batch system was studied. Medium improvement for the production employing response surface methodology (RSM) was optimized in this paper. The concentration of ...

  17. Biochemical characterization of Fusarium oxysporum f. sp. cubense ...

    African Journals Online (AJOL)

    PRECIOUS

    2010-01-25

    Jan 25, 2010 ... qualities such as taste, aroma, color, fibrous texture and nutritional content. It has a .... instance two proteins of molecular weight 51.9 and 43.0. kDa were detected only .... Earlier investigations support this view in F. oxysporum f. sp. apii ... Comparison of multi-locus enzyme and protein gel electrophoresis in.

  18. Single-Particle Soot Photometer (SP2) Instrument Handbook

    Energy Technology Data Exchange (ETDEWEB)

    Sedlacek, Arthur [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2017-02-01

    The SP2 is an instrument that measures, in situ, the time-dependent scattering and incandescence signals produced by individual BC-containing particles as they travel through a continuous-wave laser beam. Any particle traversing the laser beam will scatter light, and the BC component of a BC-containing particle will absorb some of the laser energy until its temperature is raised to the point at which it incandesces (hereafter we adopt the standard terminology of the SP2 community and denote any substance determined by the SP2 to be BC as refractory black carbon (rBC)). The amplitude of the rBC incandescence signal is related to the amount of refractory material contained in the illuminated particle. By binning the individual incandescence signals per unit sample volume, the mass concentration [ng/m3] of rBC can be derived. By binning the individual signals by volume equivalent diameter the size distribution (dN/dlogDVED) per unit time can be derived. The rBC mass loading per unit time and the rBC size distribution unit time are the core data products produced by the SP2. Additionally, the scattering channel can be used to provide information on the rBC particle population-based mixing states within ambient aerosols. However, this data product is produced on a requested-basis since additional detailed analysis and QC/QA must be conducted.

  19. Biofixation of carbon dioxide by Chlorococcum sp. in a ...

    African Journals Online (AJOL)

    The results confirmed that the photobioreactor with a membrane sparger is an alternative option for CO2 removal from flue gas by cultivation of microalgae. Keywords: Bioreactor, polytetrafluoroethene membrane sparger (PTFE) membrane sparger, Chlorococcum sp., greenhouse gas. African Journal of Biotechnology Vol.

  20. Calibration of PIXE-SP system of elementary analysis

    International Nuclear Information System (INIS)

    Tabacniks, M.H.

    1983-04-01

    The set-up and calibration of the PIXE-SP System of Elementary Analysis located at the Institute of Physics of the University of Sao Paulo is described. The PIXE-SP, that operates with 8 MeV alpha beam, has been calibrated for elements with Z >= 11, by means of homemade evaporated standard targets of Al, Si, Bacl 2 , K 2 CrO 4 , Ti, Cr, Mn, Fe, Cu, Ge, Ag, Sn, and Au. Those targets are thin, homogeneous, and have been calbrated on a microbalance within an average precision of 6%. The overall precision of the PIXE-SP System is estimated in 10% for elements with 16 2 thick, irradiated with a 10 μC electric charge, shows a minimum of about 0,1 ng (approximated 4 ppm) for Mn. The PIXE-SP System has also been intercalibrated with the PIXE located at University of Gent, Belgium, by means of 12 atmospheric aerosol targets collected on two Batelle Cascade Impactors. The analyzed masses of elements with 16 [pt