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Sample records for southern flounder paralichthys

  1. Spatial and temporal variability in growth of southern flounder (Paralichthys lethostigma)

    Science.gov (United States)

    Midway, Stephen R.; Wagner, Tyler; Arnott, Stephen A.; Biondo, Patrick; Martinez-Andrade, Fernando; Wadsworth, Thomas F.

    2015-01-01

    Delineation of stock structure is important for understanding the ecology and management of many fish populations, particularly those with wide-ranging distributions and high levels of harvest. Southern flounder (Paralichthys lethostigma) is a popular commercial and recreational species along the southeast Atlantic coast and Gulf of Mexico, USA. Recent studies have provided genetic and otolith morphology evidence that the Gulf of Mexico and Atlantic Ocean stocks differ. Using age and growth data from four states (Texas, Alabama, South Carolina, and North Carolina) we expanded upon the traditional von Bertalanffy model in order to compare growth rates of putative geographic stocks of southern flounder. We improved the model fitting process by adding a hierarchical Bayesian framework to allow each parameter to vary spatially or temporally as a random effect, as well as log transforming the three model parameters (L∞, K, andt0). Multiple comparisons of parameters showed that growth rates varied (even within states) for females, but less for males. Growth rates were also consistent through time, when long-term data were available. Since within-basin populations are thought to be genetically well-mixed, our results suggest that consistent small-scale environmental conditions (i.e., within estuaries) likely drive growth rates and should be considered when developing broader scale management plans.

  2. Commercial fishing gear modifications to reduce interactions between Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) and the southern flounder (Paralichthys lethostigma) fishery in North Carolina (USA).

    Science.gov (United States)

    Levesque, Juan C; Hager, Christian; Diaddorio, Eric; Dickey, R Jason

    2016-01-01

    Bycatch of protected species in commercial fishing operations is a primary concern to fishery managers because it threatens the conservation, protection, and recovery of fragile species, such as the Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus). One potential solution to reduce the risk associated with commercial fishing operations is to design commercial fishing gear that is more selective in terms of interactions between Atlantic sturgeon and commercial fisheries. Given this conservation and management need, the overarching goal was to reduce Atlantic sturgeon fishery interactions and maintain southern flounder (Paralichthys lethostigma) catch in North Carolina. The specific objectives of this study were to design and evaluate the effectiveness of a modified gillnet. Overall, the results proved that lowering the profile and amount of webbing had a beneficial impact at reducing Atlantic sturgeon incidental encounters and bycatch. The modified gillnet reduced bycatch and Atlantic sturgeon encounters by 39.6% and 60.9%, respectively. Our design entangled 51.6% fewer southern flounder, which corresponded to a 48.9% reduction in total weight; the modified gear entangled slightly larger southern flounder than the control gear. Our findings showed the number of Atlantic sturgeon encounters was positively associated with mean water depth, with more Atlantic sturgeon encountered in deeper (5.1-6.3 m) than shallower waters; 75% were encountered at depths between 4.6 and 6.1 m. Most southern flounder (n = 518, 39.7%) were taken at a water depth between 3.76 and 5.0 m. This observation suggests that southern flounder prefer slightly shallower waters than Atlantic sturgeon.

  3. Osmoregulation and expression of ion transport proteins and putative claudins in the gill of southern flounder (Paralichthys lethostigma)

    DEFF Research Database (Denmark)

    Tipsmark, Christian K; Luckenbach, J Adam; Madsen, Steffen S

    2008-01-01

    The southern flounder is a euryhaline teleost that inhabits ocean, estuarine, and riverine environments. We investigated the osmoregulatory strategy of juvenile flounder by examining the time-course of homeostatic responses, hormone levels, and gill Na(+),K(+)-ATPase and Na(+),K(+),2Cl(-) cotrans...... process is associated with changes in branchial expression of ion transport and putative tight junction claudin proteins known to regulate epithelial permeability in mammalian vertebrates....

  4. Histological Study on the Skin of Japanese Flounder Paralichthys olivaceus

    Institute of Scientific and Technical Information of China (English)

    ZHU Jie; ZHANG Xiumei; GAO Tianxiang

    2005-01-01

    Histological development of Japanese flounder Paralichthys olivaceus larval skin and ultrastructural difference of skin between reared normal and malpigmented Japanese flounder were studied with light microscopy (LM) and transmission electron microscopy (TEM). The results show that the skin develops slowly before the metamorphosis, while at the onset of metamorphosis, the skin develops quickly and becomes complete in structure till about 50 d after being hatched. Ultrastructural observation on the normal and malpigmented skins shows that the iridophore and melanophore are adjacent to each other. Profile and structure of the two kinds of pigment cells are more complete in the skin of normal ocular side than in the skin of pigmented blind side. The ultrastructure of typical chloride cell was observed in the skin of Japanese flounder larvae for the first time.

  5. Transcriptional responses of olive flounder (Paralichthys olivaceus to low temperature.

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    Jinwei Hu

    Full Text Available The olive flounder (Paralichthys olivaceus is an economically important flatfish in marine aquaculture with a broad thermal tolerance ranging from 14 to 23°C. Cold-tolerant flounder that can survive during the winter season at a temperature of less than 14°C might facilitate the understanding of the mechanisms underlying the response to cold stress. In this study, the transcriptional response of flounder to cold stress (0.7±0.05°C was characterized using RNA sequencing. Transcriptome sequencing was performed using the Illumina MiSeq platform for the cold-tolerant (CT group, which survived under the cold stress; the cold-sensitive (CS group, which could barely survive at the low temperature; and control group, which was not subjected to cold treatment. In all, 29,021 unigenes were generated. Compared with the unigene expression profile of the control group, 410 unigenes were up-regulated and 255 unigenes were down-regulated in the CT group, whereas 593 unigenes were up-regulated and 289 unigenes were down-regulated in the CS group. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that signal transduction, lipid metabolism, digestive system, and signaling molecules and interaction were the most highly enriched pathways for the genes that were differentially expressed under cold stress. All these pathways could be assigned to the following four biological functions for flounder that can survive under cold stress: signal response to cold stress, cell repair/regeneration, energy production, and cell membrane construction and fluidity.

  6. Determinação de idades e crescimento do linguado branco Paralichthys patagonicus (Jordan, 1889 no sul do Brasil Age and growth of the white flounder Paralichthys patagonicus (Jordan, 1889 in southern Brazil

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    Júlio Neves de Araújo

    2000-01-01

    Full Text Available A idade e o crescimento do linguado-branco Paralichthys patagonicus (Jordan, 1889 foram estudados a partir de amostras obtidas da pesca comercial no sul do Brasil entre 1989 e 1991. As idades foram determinadas sobre cortes transversais de otólitos de 460 exemplares. A análise do tipo de bordo e dos incrementos marginais dos otólitos mostrou que, a cada ano ocorre a formação de uma zona translúcida e uma zona opaca nos otólitos. As "remeas atingem maiores tamanhos e idades (630 mm, 13 anos do que os machos (527 mm, 11 anos. Os parâmetros da equação de von Bertalanffy que representaram o crescimento em comprimento total das remeas foram: CT∞,= 623,0 mm, K= 0,279 e to= -0,243 anos; dos machos: CT∞,= 534,1 mm, K= 0,214 e t o = -1,589 anos e dos sexos agrupados: CT∞, = 609,1 mm, K = 0,255 to = - 0,462 anos. Os incrementos anuais em peso por idades aumentam até o final do quarto ano de vida quando a maioria já atingiu a primeira maturação sexual e ambos sexos alcançaram cerca de 30% do peso e 70% do comprimento assintóticos. Esta espécie é susceptível à sobrepesca de crescimento porque o redirecionamento do metabolismo do crescimento somático para a reprodução é gradativo e o potencial de crescimento após a maturação sexual é relativamente alto.Age and growth of the white flounder Paralichthys patagonicus (Jordan, 1889 were studied using sliced sagittae otoliths of 460 specimens collected from the commercial fishery along southem Brazil (1989 to 1991. The changes in the type of last band in formation and the marginal increments analysis showed that one translucent and one opaque zone were formed each year. Females attained larger totallength (TL and older ages (630 mm, 13 yr than males (527 mm, 11 yr. The parameters ofthe von Bertalanffy growth equations were: for females - TL∞,= 623.0 mm, K= 0.279 and t o = - 0.243 yr; for males - TL∞,= 534.1 mm, K= 0.214, to = - 1.589 yr and for pooled sexes - TL∞, = 609.1 mm

  7. Multi-decadal variation in size of juvenile Summer Flounder (Paralichthys dentatus) in Chesapeake Bay

    Science.gov (United States)

    Nys, Lauren N.; Fabrizio, Mary C.; Tuckey, Troy D.

    2016-01-01

    During the last quarter-century, management of Summer Flounder Paralichthys dentatus along the Atlantic coast resulted in significant increases in abundance such that rebuilding targets were recently achieved. Although spawning stock biomass is high, recruitment of young-of-the-year (YOY) Summer Flounder remains variable. Chesapeake Bay is one of the principal nursery areas for this species, but processes such as growth and survival that affect production of YOY Summer Flounder in this estuary have not been explored. Here, we investigated the relationship between abundance and size of Summer Flounder recruits from the 1988 to 2012 year classes in Chesapeake Bay. We also considered the effects of environmental factors on fish size because conditions in the bay vary spatially during the time that fish occupy nursery areas. To describe variations in Summer Flounder size, we used monthly length observations from 13,018 YOY fish captured by bottom trawl from the lower Chesapeake Bay and the James, York, and Rappahannock river subestuaries where Summer Flounder are commonly observed. We applied a generalized additive model to describe spatial, temporal, and environmental effects on observed fish size; we also considered the density of Summer Flounder and an index of productivity as factors in the model. Summer Flounder in Chesapeake Bay exhibited density-dependent and spatially related variations in mean length: larger fish were found mostly in the Bay and smaller fish in the subestuaries. Additionally, low ( 26 °C) temperatures and low salinities (indicating that individuals found in these environments were typically smaller than conspecifics inhabiting areas of moderate temperatures and higher salinities. Variable nursery habitat conditions in temperate estuaries affect fish size and, subsequently, may influence production of Summer Flounder year classes through effects on maturation and survival. As water temperatures in the mid-Atlantic region continue to increase

  8. The toxic mechanism of high lethality of herbicide butachlor in marine flatfish flounder, Paralichthys olivaceus

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    Guo, Huarong; Yin, Licheng; Zhang, Shicui; Feng, Wenrong

    2010-09-01

    The toxic mechanism of herbicide butachlor to induce extremely high lethality in marine flatfish flounder, Paralichthys Olivaceus, was analyzed by histopathological examination, antioxidant enzymes activities and ATP content assay. Histopathological examination of gill, liver and kidney of exposed fishes showed that gill was a target organ of butachlor. The butachlor seriously impaired the respiration of gills by a series of lesions such as edema, lifting and detachment of lamellar epithelium, breakdown of pillar cells, and blood congestion. The dysfunction of gill respiration caused suffocation to the exposed flounder with extremely high acute lethality. Antioxidant enzyme activity assay of the in vitro cultured flounder gill (FG) cells exposed to butachlor indicated that butachlor markedly inhibited the antioxidant enzyme activities of Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX). Furthermore, along with the decline of antioxidant enzyme activities, ATP content in the exposed FG cells decreased, too. This infers that the oxidative stress induced by butachlor can inhibit the production of cellular ATP. Similar decrease of ATP content was also observed in the exposed flounder gill tissues. Taken together, as in FG cells, butachlor possibly induced a short supply of ATP in pillar cells by inhibiting the antioxidant enzyme activities and then affecting the contractibility of the pillar cells, which in turn resulted in the blood congestion and suffocation of exposed flounder.

  9. Molecular cloning and expression analysis of the STAT1 gene from olive flounder, Paralichthys olivaceus

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    Chung Jongkyeong

    2008-06-01

    Full Text Available Abstract Background Signal transducer and activator of transcription 1 (STAT1 is a critical component of interferon (IFN-alpha/beta and IFN-gamma signaling. Although seven isoforms of STAT proteins have been reported from mammals, limited information is available for the STAT genes in fish. We isolated complementary DNA with high similarity to mammalian STAT1 from the olive flounder, Paralichthys olivaceus. Results A DNA fragment containing the conserved SH2 domain was amplified by RT-PCR using degenerate primers designed based on the highly conserved sequences in the SH2 domains of the zebrafish and mammalian STAT1. The complete cDNA sequence was obtained by 5' and 3' RACE. The flounder STAT1 transcript consisted of 2,909 bp that encoded a polypeptide of 749 amino acids. The overall similarity between flounder STAT1 and other STATs was very high, with the highest amino acid sequence identity to snakehead (89%. Phylogenetic analyses reveal that flounder STAT1 is in the same monophyletic group with snakehead STAT1. Quantitative real time RT-PCR and in situ hybridization revealed that STAT1 was expressed in almost all examined organs and tissues, with high expression in gill, spleen, kidney, and heart. The accumulation of STAT1 mRNA in different developmental stages, as determined by real time RT-PCR, increased with development. Conclusion Recent cloning of various cytokine genes and the STAT1 gene of olive flounder here suggest that fish also use the highly specialized JAK-STAT pathway for cytokine signaling. Identification of other STAT genes will elucidate in detail the signal transduction system in this fish.

  10. Protective efficiency of an inactivated vaccine against Streptococcus iniae in olive flounder, Paralichthys olivaceus

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    Jeong Yong-Uk

    2016-03-01

    Full Text Available Streptococcus iniae is a causative agent of hemorrhagic septicemia in olive flounder, Paralichthys olivaceus, in Korea, resulting in serious economic losses. As a preventive measure, M VAC INIAE (Mastuken, Japan was prepared from the S. iniae F2K strain and tested against the SI-36 strain prevalent on flounder fish farms on Jeju Island, Korea. F2K had a serotype of 38 (− and SI-36 38 (+. The vaccine recognized both serotypes. It showed a very high effective immune response against S. iniae; the challenge test using the S. iniae SI-36 strain resulted in a relative percent survival (RPS of 85.7-87.0% 2 weeks after vaccination and 71.0-80.0% 6 months after vaccination. Field vaccination and clinical challenge tests were performed at local Jeju aquafarms with S. iniae SI-36. These showed significantly reduced cumulative mortality when compared to the control group with RPS rates that ranged between 71-80%. Hence, the present study suggests that this vaccine showed a significant immune response against S. iniae and could be applied in commercial aquafarms as a therapeutic agent against β-hemolytic streptococcosis in cultured P. olivaceus.

  11. LAMP-1-chimeric DNA vaccines enhance the antibody response in Japanese flounder, Paralichthys olivaceus.

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    Rondón-Barragán, Iang; Nozaki, Reiko; Hirono, Ikuo; Kondo, Hidehiro

    2017-08-01

    DNA vaccination is one method to protect farmed fish from viral and bacterial diseases. Chimeric antigens encoded by DNA vaccines have been shown to increase the resistance to viral diseases. Here, we sequenced the gene encoding lysosome-associated membrane protein-1 from Japanese flounder, Paralichthys olivaceus, (JfLAMP-1) and assessed its use in a chimeric DNA vaccine fused with the major capsule protein (MCP) from red seabream iridovirus (RSIV). JfLAMP-1 cDNA has a length of 1248 bp encoding 415 aa, which contains transmembrane and cytoplasmic domains. JfLAMP-1 is constitutively expressed in several tissues and its expression in spleen was upregulated following injection of formalin-killed cells (FKC) of Edwardsiella tarda. Immunofluorescence analysis showed that JfLAMP-1 is distributed in the small and large granules in the cytoplasm and groups close to the nucleus. The DNA encoding the luminal domain of JfLAMP-1 was replaced with the gene for the RSIV MCP, and the construct was cloned in an expression vector (pCIneo). Fish vaccinated with pCLAMP-MCP had significantly higher antibody levels than fish vaccinated with pCIneo vector harboring the MCP gene (p day 30 post-vaccination. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Detection of lymphocystis disease virus in Japanese flounder Paralichthys olivaceus and other marine teleosts from northern China

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    Zhan, Wenbin; Li, Yongqin; Sheng, Xiuzhen; Xing, Jing; Tang, Xiaoqian

    2010-11-01

    We isolated a strain of lymphocystis disease virus (LCDV) from Japanese flounder ( Paralichthys olivaceus) cultured in northern China. Based on published sequences of major capsid protein (MCP) gene of LCDV-cn (GenBank: AF126405), we designed two primer sets P1/P2 and P3/P4. We then used one-step or nested PCR and in-situ hybridization (ISH) to detect LCDV and identify the target tissues or cells in infected Japanese flounder. The PCR products were positive in purified viral supernatant, skin nodules, gut, gill, kidney, spleen, stomach, heart, and liver of Japanese flounder. We compared the DNA sequence with 14 MCP nucleotide sequences from GenBank, including Megalocytivirus (OFIV and RSIV), Iridovirus (CzIV and WIV), Ranavirus (TFV and FV3), and Lymphocystivirus (8 LCDV). Based on the alignment, we confirmed the PCR product was from Lymphocystivirus (GenBank accession number DQ279090 (LCDV-HD)). Using ISH, we noted the presence of LCDV in the skin nodules, gut, gill, spleen, stomach, and heart of spontaneously infected Japanese flounders. We successfully amplified LCDV fragments from Schlegel’s black rockfish ( Sebastes schlegeli Higendorf), redwing sea robin ( Lepidotrigla microptera Günther) and turbot ( Scophthalmus maximus) using the one-step and nested PCR, suggesting the target genes can be widely detected in fish using this method.

  13. Population discrimination by strontium-calcium concentration ratios of sagittal otoliths taken from the Japanese flounder, Paralichthys olivaceus

    International Nuclear Information System (INIS)

    Kakuta, I.; Chiba, D.; Ishii, K.; Yamazaki, H.; Iwasaki, S.; Matsuyama, S.

    1999-01-01

    For the purpose of obtaining basic data to understand the population dynamics of the Japanese flounder, Paralichthys olivaceus, inhabiting the Sanriku coastal waters, the concentration ratios of Ca and Sr in otoliths of juvenile fishes being cultivated for releasing to the regions, and those of adult fishes captured in both the Sanriku area (Aomori, Iwate and Miyagi) and Shizuoka prefecture coastal regions (as a comparison) were analysed using a particle induced X-ray emission (PIXE) technique. The Sr/Ca ratios of otoliths taken from juvenile Japanese flounders had significant differences between Sanriku and Shizuoka sea-farming groups. The differences in otolith Sr/Ca ratios between Sanriku and Shizuoka sea-farming stations would thus differentiate flounder populations. No significant difference in otolith Sr/Ca ratios was observed among the Sanriku group. However, the values for Aomori group formed by small fishes in the Sanriku group seemed to be lower in proportion to their body size. Therefore, genetic characteristics of the juvenile Japanese flounder being reared at the sea-farming stations in Iwate and Miyagi prefectures are possibly different from those at an Aomori station. On the other hand, statistically significant differences in the otolith Sr/Ca ratios among Aomori, Iwate Miyagi and Shizuoka groups were found in the adult Japanese flounder. That is, higher values for the otolith Sr/Ca ratios were found in the groups inhabiting in the northern regions. The differences in otolith Sr/Ca ratios among these groups probably indicate that there are differences in the fish populations among these sample sites. (author)

  14. Cadmium accumulation and elimination in tissues of juvenile olive flounder, Paralichthys olivaceus after sub-chronic cadmium exposure

    International Nuclear Information System (INIS)

    Kim, Seong-Gil; Jee, Jung-Hoon; Kang, Ju-Chan

    2004-01-01

    Cd accumulation and elimination in tissue of olive flounder. - Experiments were carried out to investigate the accumulation and elimination of cadmium (Cd) in tissues (gill, intestine, kidney, liver and muscle) of juvenile olive flounder, Paralichthys olivaceus, exposed to sub-chronic concentrations (0, 10, 50, 100 μg l -1 ) of Cd. Cd exposure resulted in an increased Cd accumulation in tissues of flounder with exposure periods and concentration, and Cd accumulation in gill and liver increased linearly with the exposure time. At 20 days of Cd exposure, the order of Cd accumulation in organs was gill > intestine > liver > kidney > muscle and after 30 days of exposure, those were intestine > gill > liver > kidney > muscle. An inverse relationship was observed between the accumulation factor (AF) and the exposure level, but AF showed an increase with exposure time. During the depuration periods, Cd concentration in the gill, intestine and liver decreased immediately following the end of the exposure periods. No significant difference was found Cd in concentration in the kidney and muscle during depuration periods. The order of Cd elimination rate in organs were decreased intestine > liver > gill during depuration periods

  15. De Novo assembly of the Japanese flounder (Paralichthys olivaceus spleen transcriptome to identify putative genes involved in immunity.

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    Lin Huang

    Full Text Available Japanese flounder (Paralichthys olivaceus is an economically important marine fish in Asia and has suffered from disease outbreaks caused by various pathogens, which requires more information for immune relevant genes on genome background. However, genomic and transcriptomic data for Japanese flounder remain scarce, which limits studies on the immune system of this species. In this study, we characterized the Japanese flounder spleen transcriptome using an Illumina paired-end sequencing platform to identify putative genes involved in immunity.A cDNA library from the spleen of P. olivaceus was constructed and randomly sequenced using an Illumina technique. The removal of low quality reads generated 12,196,968 trimmed reads, which assembled into 96,627 unigenes. A total of 21,391 unigenes (22.14% were annotated in the NCBI Nr database, and only 1.1% of the BLASTx top-hits matched P. olivaceus protein sequences. Approximately 12,503 (58.45% unigenes were categorized into three Gene Ontology groups, 19,547 (91.38% were classified into 26 Cluster of Orthologous Groups, and 10,649 (49.78% were assigned to six Kyoto Encyclopedia of Genes and Genomes pathways. Furthermore, 40,928 putative simple sequence repeats and 47, 362 putative single nucleotide polymorphisms were identified. Importantly, we identified 1,563 putative immune-associated unigenes that mapped to 15 immune signaling pathways.The P. olivaceus transciptome data provides a rich source to discover and identify new genes, and the immune-relevant sequences identified here will facilitate our understanding of the mechanisms involved in the immune response. Furthermore, the plentiful potential SSRs and SNPs found in this study are important resources with respect to future development of a linkage map or marker assisted breeding programs for the flounder.

  16. Tissue-specific bioaccumulation and oxidative stress responses in juvenile Japanese flounder ( Paralichthys olivaceus) exposed to mercury

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    Huang, Wei; Cao, Liang; Ye, Zhenjiang; Lin, Longshan; Chen, Quanzhen; Dou, Shuozeng

    2012-07-01

    To understand mercury (Hg) toxicity in marine fish, we measured Hg accumulation in juvenile Japanese flounder ( Paralichthys olivaceus) and assessed the effects on growth and antioxidant responses. After Hg exposure (control, 5, 40, and 160 μg/L Hg) for 28 d, fish growth was significantly reduced. The accumulation of Hg in fish was dose-dependent and tissue-specific, with the maximum accumulation in kidney and liver, followed by gills, bone, and muscle. Different antioxidants responded differently to Hg exposure to cope with the induction of lipid peroxidation (LPO), which was also tissue-specific and dosedependent. As Hg concentration increased, superoxide dismutase (SOD) and catalase (CAT) activities increased significantly, whereas glutathione S -transferase (GST) activity and glutathione (GSH) levels decreased significantly in the gills. SOD and glutathione peroxidase (GPx) activities and the GSH level increased significantly in the liver. SOD activity and GSH levels increased significantly, but CAT activity decreased significantly with an increase in Hg concentration in the kidney. LPO was induced significantly by elevated Hg in the gills and kidney but was least affected in the liver. Therefore, oxidative stress biomarkers in gills were more sensitive than those in the liver and kidney to Hg exposure. Thus, the gills have potential as bioindicators for evaluating Hg toxicity in juvenile flounder.

  17. Disruption of the thyroid system by the thyroid-disrupting compound Aroclor 1254 in juvenile Japanese flounder (Paralichthys olivaceus.

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    Yifei Dong

    Full Text Available Polychlorinated biphenyls (PCBs are a group of persistent organochlorine compounds that have the potential to disrupt the homeostasis of thyroid hormones (THs in fish, particularly juveniles. In this study, thyroid histology, plasma TH levels, and iodothyronine deiodinase (IDs, including ID1, ID2, and ID3 gene expression patterns were examined in juvenile Japanese flounder (Paralichthys olivaceus following 25- and 50-day waterborne exposure to environmentally relevant concentrations of a commercial PCB mixture, Aroclor 1254 (10, 100, and 1000 ng/L with two-thirds of the test solutions renewed daily. The results showed that exposure to Aroclor 1254 for 50 d increased follicular cell height, colloid depletion, and hyperplasia. In particular, hypothyroidism, which was induced by the administration of 1000 ng/L Aroclor 1254, significantly decreased plasma TT4, TT3, and FT3 levels. Profiles of the changes in mRNA expression levels of IDs were observed in the liver and kidney after 25 and 50 d PCB exposure, which might be associated with a reduction in plasma THs levels. The expression level of ID2 mRNA in the liver exhibited a dose-dependent increase, indicating that this ID isotype might serve as sensitive and stable indicator for thyroid-disrupting chemical (TDC exposure. Overall, our study confirmed that environmentally relevant concentrations of Aroclor 1254 cause significant thyroid disruption, with juvenile Japanese flounder being suitable candidates for use in TDC studies.

  18. Effect of dietary carbohydrate sources on apparent nutrient digestibility of olive flounder (Paralichthys olivaceus feed

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    Md Mostafizur Rahman

    2016-06-01

    Full Text Available Abstract Apparent digestibility coefficients (ADCs of dry matter, crude protein, crude lipid, nitrogen-free extract, and energy in selected carbohydrate sources including wheat flour (WF, α-potato starch (PS, α-corn starch (CS, Na alginate (AL, dextrin (DEX, and carboxymethyl cellulose (CMC were determined for olive flounder. The olive flounder averaging 150 ± 8.0 g were held in 300-L tanks at a density of 30 fish per tank. Chromic oxide was used as the inert marker. Feces were collected from the flounder by a fecal collector attached to a fish rearing tank. Apparent dry matter and energy digestibilities of flounder fed WF, PS, CS, and DEX diets were significantly higher than those of fish fed AL and CMC diets. Apparent crude protein digestibility coefficients of flounder fed PS and CS diets were significantly higher than those of fish fed AL, DEX, and CMC diets. Apparent crude lipid and nitrogen-free extract digestibility coefficients of flounder fed PS and DEX diets were significantly higher than those of fish fed WF, CS, AL, and CMC diets. The present findings indicate that PS and DEX could be effectively used as dietary carbohydrate energy compared to WF, CS, AL, and CMC for olive flounder.

  19. Characterization of the intestinal microbiota of wild-caught and farmed fine flounder (Paralichthys adspersus)

    OpenAIRE

    Salas-Leiva, Joan; Opazo, Rafael; Remond, Camille; Uribe, Eduardo; Velez, Antonio; Romero, Jaime

    2017-01-01

    A comparative analysis of the cultivable intestinal microbiota of farmed (AF) and wild-caught (WF) Paralichthys adspersus was performed. The 16S rRNA gene was used for taxa identification, and the ITS region for strain differentiation. We detected the presence of Vibrio, Bacillus, Photobacterium, Staphylococcus and Carnobacterium in AF, and Exiguobacterium, Klebsiella, Arthrobacter, Raoultella, Kluyvera, Myroides, Streptococcus, Vagococcus, Staphylococcus, Acinetobacter, Psychrobacter, Lactob...

  20. Effects of extruded pellet and moist pellet on growth performance, body composition, and hematology of juvenile olive flounder, Paralichthys olivaceus

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    Seunghan Lee

    2016-10-01

    Full Text Available Abstract A feeding trial was conducted to evaluate the effects of two different sizes of extruded pellets (EP (EP1 - 3 mm or EP2 - 5 mm and a moist pellet (MP in olive flounder, Paralichthys olivaceus, reared in semi-recirculation system. A total of 450 fish with an average initial weight of 5.0 ± 0.2 g (mean ± SD were fed one of the three experimental diets in triplicate groups. At the end of a 6-week feeding trial, weight gain, specific growth rate, and feed efficiency of fish fed EP diets were significantly higher than those of fish fed MP (P < 0.05. Water quality parameters like turbidity, total ammonia nitrogen, and total phosphorous from tanks of fish fed EP1 and EP2 were significantly lower than those from tanks of fish fed MP. Blood plasma glutamic oxaloacetic transaminase and glucose concentration were significantly higher in fish fed MP diet compared to fish fed EP diets (P < 0.05. Whole body crude protein contents in fish fed EP diets were higher than those from the fish fed MP diet. Whole body amino acid content like threonine, aspartic acid, serine, tyrosine, and cystine were found to be significantly higher in fish fed EP diets than those in fish fed MP diet. In considering overall performance of olive flounder, EP2 diet could be recommended for the successful aquaculture of this important fish species.

  1. Effects of hydrostatic pressure on microtubule organization and nucleus changes in gynogenetically activated eggs of olive flounder (Paralichthys olivaceus).

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    Lin, Zhengmei; Zhu, Xiangping; Zhang, Tingrong; You, Feng; Wu, Zhihao; Cao, Yuanshui

    2016-06-01

    Fluorescent double-labeled technique was used to investigate the effects of hydrostatic pressure on microtubule organization and nucleus in gynogenetically activated eggs of olive flounder (Paralichthys olivaceus). The parameter of hydrostatic pressure treatment was 600 kg/cm(2) for 6 minutes at prometaphase of the first mitosis. The data showed that nucleus and microtubule changes of the diploid control were basically similar to those of the haploid one (5 minutes behind those of the diploid control). Nuclear diameter of the haploid embryo was significantly smaller than that of the diploid one (P pressure treatment. Centrosome renucleated microtubule, and a bipolar spindle reassembled 15 minutes after treatment, leading to occurrence of the first cleavage. During the second cell cycle, about 80% treated embryo had a single centrosome and formed a unipolar spindle in both blastomeres. After prometaphase, chromosomes spread around for about 20 minutes instead of aligning on the equatorial plane, then assembled and formed one large nucleus without anaphase separation. The second cleavage was inhibited, and the chromosome set was doubled. The data indicated that the chromosome set doubling of mitogynogenetic diploid induced by hydrostatic pressure treatment, which performed at prometaphase of the first mitosis, mainly resulted from the inhibition of the second cleavage rather than the first one. This study is the first to adapt fluorescent double-labeled technique to investigate the mechanism on chromosome set doubling of mitotic gynogenesis induction. This study will offer theoretical support for mitogynogenetic diploid induction in marine fish. Copyright © 2016 Elsevier Inc. All rights reserved.

  2. Possible Involvement of Photoperiodic Regulation in Reproductive Endocrine System of Female Olive Flounder Paralichthys olivaceus.

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    Kim, Hyun Chul; Lee, Chi Hoon; Hur, Sung Pyu; Kim, Byeong Hoon; Park, Jun Young; Lee, Young Don

    2015-03-01

    This study investigated possible involvement of photoperiodic regulation in reproductive endocrine system of female olive flounder. To investigate the influence on brain-pituitary axis in endocrine system by regulating photoperiod, compared expression level of Kisspeptin and sbGnRH mRNA in brain and FSH-β, LH-β and GH mRNA in pituitary before and after spawning. Photoperiod was treated natural photoperiod and long photoperiod (15L:9D) conditions from Aug. 2013 to Jun. 2014. Continuous long photoperiod treatment from Aug. (post-spawning phase) was inhibited gonadal development of female olive flounder. In natural photoperiod group, the Kiss2 expression level a significant declined in Mar. (spawning period). And also, FSH-β, LH-β and GH mRNA expression levels were increasing at this period. However, in long photoperiod group, hypothalamic Kiss2, FSH-β, LH-β and GH mRNA expression levels did not show any significant fluctuation. These results suggest that expression of hypothalamic Kiss2, GtH and GH in the pituitary would change in response to photoperiod and their possible involvement of photoperiodic regulation in reproductive endocrine system of the BPG axis.

  3. Effects of calanoid copepod Schmackeria poplesia as a live food on the growth, survival and fatty acid composition of larvae and juveniles of Japanese flounder, Paralichthys olivaceus

    Science.gov (United States)

    Liu, Guangxing; Xu, Donghui

    2009-12-01

    Zooplankton constitutes a major part of the diet for fish larvae in the marine food web, and it is generally believed that copepods can meet the nutritional requirements of fish larvae. In this study, calanoid copepod Schmackeria poplesia, rotifer Brachionus plicatilis and anostraca crustacean Artemia sp. were analyzed for fatty acid contents, and were used as live food for culturing larval Japanese flounder, Paralichthys olivaceus. The total content of three types of HUFAs (DHA, EPA and ARA) in S. poplesia was significantly higher than that in the other two live foods ( P<0.01). Three live organisms were used for raising larvae and juveniles of Paralichthys olivaceus respectively for 15 and 10 d. Then the growth, survival and fatty acid composition of the larvae and juveniles were investigated. The results showed that the larvae and juveniles fed with copepods ( S. poplesia) had significantly higher growth rate than those fed with the other two organisms ( P<0.01). The survival of the flounder larvae fed with copepods was significantly higher than that of the others ( P<0.01), and the survival of the juvenile fish fed with copepods was higher than that fed with Artemia ( P<0.05). The contents of three types of HUFAs (DHA, EPA and ARA) and the ratio of DHA/EPA in larval and juvenile flounder P. olivaceus were analyzed. The results showed that the contents of DHA, EPA and ARA in the larvae and juveniles fed with S. poplesia were higher than those fed with a mixed diet or Artemia only, and the ratio of EPA/ARA in larvae and juveniles of P. olivaceus fed with S. poplesia was lower than that in the case of feeding with a mixed diet or Artemia only. The present data showed that copepod is the best choice for feeding the larvae and juveniles of fish considering its effects on the survival, growth and nutrition composition of the fish.

  4. Changes of cytochrome P4501A mRNA expression and physiology responses in the olive flounder, Paralichthys olivaceus, exposed to benzo(a)pyrene

    Energy Technology Data Exchange (ETDEWEB)

    Choi, C.Y.; An, K.W.; Shin, H.S.; An, M.I.; Jo, P.G. [Korean Maritime University, Pusan (Republic of Korea). Division of Marine Environmental and Bioscience

    2008-07-01

    Benzo(a)pyrene (BaP) is generated by the incomplete combustion of organic substances such as oil and coal, and is a widespread organic environmental contaminant in terrestrial and aquatic ecosystems. To determine the effects of BaP on organisms, we investigated its time- and dose-related effects on the levels of cytochrome P4501A (P4501A) mRNA in the liver and gills of the olive flounder (Paralichthys olivaceus) using quantitative polymerase chain reaction (QPCR) and measured the plasma glucose, cortisol, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels. The full-length olive flounder P4501A cDNA consists of 1566 nucleotides and encodes a 521-amino-acid protein. In the liver and gills, the expression of P4501A mRNA was highest 6 h after exposure to both 10 and 30 gl{sup -1} BaP, and then decreased. In addition, the plasma parameters increased with exposure. These results suggest that P4501A plays an important role in the detoxification of BaP, which stressed the olive flounder. Therefore, these physiological parameters may be indicators of BaP-induced stress responses.

  5. In vitro inhibition of fish rhabdoviruses by Japanese flounder, Paralichthys olivaceus Mx

    International Nuclear Information System (INIS)

    Caipang, Christopher Marlowe A.; Hirono, Ikuo; Aoki, Takashi

    2003-01-01

    A homologous fish cell line stably expressing the recombinant Japanese flounder Mx (JFMx) was infected with hirame rhabdovirus (HIRRV) and viral hemorrhagic septicemia virus (VHSV), both of which are negative single-stranded RNA viruses belonging to the Rhabdoviridae family. Analysis of primary transcription of the two rhabdoviruses showed that there was lower expression level and copy number of the viral nucleoprotein transcript in the JFMx-transfected cell line than the infected, control cells, although no significant difference was observed. This suggests that JFMx may not be a potent inhibitor of rhabdoviral primary transcription. Kinetics of rhabdovirus expression by RT-PCR and quantitative real-time RT-PCR showed reduced levels of the rhabdoviral glycoprotein and nucleoprotein transcripts over time, indicating the possible role of JFMx in blocking rhabdoviral replication by interfering with the transcription of the viral subgenomic mRNAs. Significant inhibition in rhabdovirus replication consequently resulted in the synthesis of fewer viral particles. This may explain why JFMx-expressing cells are less susceptible to virus-induced cell lysis, and thus, why they would have a significantly higher survival than the infected, control cells. These results provide direct evidence that JFMx has an antiviral effect in vitro

  6. An application of clonal Japanese flounder, paralichthys olivaceus to the experiment on bioaccumulation of radionuclides

    International Nuclear Information System (INIS)

    Miyazaki, Taeko; Nakahara, Motokazu; Nakamura, Ryoichi; Hirano, Shigeki; Watabe, Teruhisa; Yamamoto, Eiichi

    2000-01-01

    A laboratory experiment of accumulation and excretion of radionuclides ( 125 I, 57 Co, 141 Ce, 103 Ru, 85 Sr, 137 Cs, 54 Mn) in juvenile Japanese flounder was carried out in order to elucidate the genetic factor intervening in the mineral balance, which possibly caused the fluctuation of so-called concentration factors. Fish originating from two populations, namely clone brood and hatchery-reared brood, were used in the present study. The variance of concentration of radionuclides was compared in terms of the radionuclides activity ratio, which was defined by radionuclide concentration in fish normalized by that in surrounding water. In general, narrower variations of concentration were observed in clone brood than in hatchery-reared brood, and significant differences were observed for 57 Co, 141 Ce and 137 Cs in the accumulation process and for five radionuclides other than 125 I and 103 Ru in excretion process. The present study suggested that a probability of application of clonal fish would be advantageous in experimental assessments of biological effects of environmental contaminants in the sea. (author)

  7. Preparation and Characterization of an Olive Flounder (Paralichthys olivaceus) Skin Gelatin and Polylactic Acid Bilayer Film.

    Science.gov (United States)

    Lee, Ka-Yeon; Song, Kyung Bin

    2017-03-01

    Olive flounder skin gelatin (OSG) was used as a film base material. A bilayer film of OSG and polylactic acid (PLA) was prepared using solvent casting method to enhance the film properties. Physical properties of the OSG-PLA film were increased compared with the nonaugmented OSG film. In particular, the PLA lamination decreased water vapor permeability from 2.17 to 0.92 × 10 -9 g·m/m 2 ·s·Pa, as well as of the water solubility from 16.62% to 9.27%, in the bilayer film relative to the OSG film. The oxygen permeability of the OSG-PLA bilayer film was held low by the OSG film, compensating for the high oxygen permeability of the PLA layer. Therefore, the OSG-PLA bilayer film with its enhanced physical properties and high water and oxygen barrier properties can be applied as a food packaging material. © 2017 Institute of Food Technologists®.

  8. Antigen uptake and expression of antigen presentation-related immune genes in flounder (Paralichthys olivaceus) after vaccination with an inactivated Edwardsiella tarda immersion vaccine, following hyperosmotic treatment.

    Science.gov (United States)

    Gao, Yingli; Tang, Xiaoqian; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin

    2016-08-01

    Antigen uptake is a critical process for activation of the immune system, and therefore the ability to enhance antigen uptake is a primary consideration in the development of an immersion vaccination of fish. In the present work, flounders (Paralichthys olivaceus) were immersed in three hyperosmotic solutions with 40, 50 and 60‰ salinities, then transferred into seawater of normal salinity (i.e. 30‰) containing formalin-inactivated Edwardsiella tarda for 30 min. The antigen uptake in vaccinated flounder was determined using an absolute quantitative PCR (qPCR). The results showed significantly higher antigen uptake in the tissues of flounders immersed in solutions with 50‰ and 60‰ salinity compared to the control group directly immersed in vaccine (DI) (P immersed in the 50‰ salinity solution, whereas there was no significant difference in antigen uptake between the 40‰ salinity group and the DI group (P > 0.05). A rapid and significant increase in antigen uptake was detected in the mucosal-associated tissues including the gill, skin and intestine (P immersion, which was significantly higher than the levels of uptake measured in the other tissues (P immersion (hpi). The expression profiles of four antigen presentation-related immune genes (MHC Iα, MHC IIα, CD4-1 and CD8α) were investigated after immersion. These four genes showed a significantly stronger response in the immersed flounders exposed to 50‰ salinity compared with the DI group (P immersion, notably 50‰ salinity significantly enhanced antigen uptake and the expression of selected genes associated with antigen presentation, providing evidence for an enhanced immune activation of the fish's immune response by the hyperosmotic immersion treatment prior to vaccination. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Characteristics of the interferon regulatory factor 5 (IRF5) and its expression in response to LCDV and poly I:C challenges in Japanese flounder, Paralichthys olivaceus.

    Science.gov (United States)

    Hu, Guo-Bin; Lou, Hui-Min; Dong, Xian-Zhi; Liu, Qiu-Ming; Zhang, Shi-Cui

    2012-10-01

    Interferon regulatory factor 5 (IRF5) has been identified as a key transcriptional mediator regulating expression of both type I interferons (IFNs) and proinflammatory cytokines. In this study, the cDNA and genomic sequences of IRF5 were isolated from Japanese flounder, Paralichthys olivaceus. The gene of Japanese flounder (Jf)IRF5 is 7326 bp long, contains 9 exons and 8 introns and encodes a putative protein of 472 amino acids. The predicted protein sequence shares 61.1-81.9% identity to fish IRF5 and possesses a DNA-binding domain (DBD), a middle region (MR), an IRF association domain (IAD), a virus activated domain (VAD) and two nuclear localization signals (NLSs) conserved in all known IRF5s. Phylogenetic analysis clustered it into the teleost IRF5 subgroup within vertebrate IRF5 group. JfIRF5 mRNA was constitutively expressed in all tissues examined, with higher levels observed in the gills and head kidney. Gene expression of JfIRF5 was analyzed over a 7-day time course in the gills, head kidney, spleen and muscle of Japanese flounders challenged with lymphocystis disease virus (LCDV) and polyinosinic:polycytidylic acid (poly I:C). The data showed that JfIRF5 expression was slightly up-regulated by LCDV, but its induction time was clearly moved up; in contrast, the induction upon poly I:C challenge started not earlier than day 2 post-injection and was stronger and more persistent with a later peak time in all four organs. The late and long-lasting inductive expression of JfIRF5 following poly I:C challenge suggests that it might be an interferon stimulated gene (ISG), the induction of which is driven by poly I:C-induced type I IFNs. Copyright © 2012 Elsevier Ltd. All rights reserved.

  10. Expression of insulin-like growth factor I receptors at mRNA and protein levels during metamorphosis of Japanese flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Zhang, Junling; Shi, Zhiyi; Cheng, Qi; Chen, Xiaowu

    2011-08-01

    Insulin-like growth factor I (IGF-I) is an important regulator of fish growth and development, and its biological actions are initiated by binding to IGF-I receptor (IGF-IR). Our previous study has revealed that IGF-I could play an important role during metamorphosis of Japanese flounder, Paralichthys olivaceus. The analysis of IGF-IR expression thus helps further elucidate the IGF-I regulation of metamorphic processes. In this study, the spatial-temporal expression of two distinct IGF-IR mRNAs was investigated by real-time RT-PCR. The spatial distribution of two IGF-IR mRNAs in adult tissues is largely overlapped, but they exhibit distinct temporal expression patterns during larval development. A remarkable decrease in IGF-IR-2 mRNA was detected during metamorphosis. In contrast, a significant increase in IGF-IR-1 mRNA was determined from pre-metamorphosis to metamorphic completion. These indicate that they may play different function roles during the flounder metamorphosis. The levels and localization of IGF-IR proteins during larval development were further studied by Western blotting and immunohistochemistry. Immunoreactive IGF-IRs were detected throughout larval development, and the IGF-IR proteins displayed a relatively abundant expression during metamorphosis. Moreover, the IGF-IR proteins appeared in key tissues, such as thickened skin beneath the migrating eye, developing intestine, gills and kidney during metamorphosis. These results further suggest that the IGF-I system may be involved in metamorphic development of Japanese flounder. Copyright © 2011 Elsevier Inc. All rights reserved.

  11. Comparative analysis of distinctive transcriptome profiles with biochemical evidence in bisphenol S- and benzo[a]pyrene-exposed liver tissues of the olive flounder Paralichthys olivaceus.

    Directory of Open Access Journals (Sweden)

    Jee-Hyun Jung

    Full Text Available Flounder is a promising model species for environmental monitoring of coastal regions. To assess the usefulness of liver transcriptome profiling, juvenile olive flounder Paralichthys olivaceus were exposed to two pollutants, bisphenol S (BPS and benzo[a]pyrene (BaP, which have different chemical characteristics and have distinct modes of metabolic action in teleost. Six hours after intraperitoneal injection with BPS (50 mg/kg bw or BaP (20 mg/kg bw, liver transcriptomes were analyzed using the Illumina Hiseq 3000 platform. Interestingly, the transcriptome was highly sensitive and was distinctively expressed in response to each chemical. The primary effect of BPS was significantly increased transcription of egg process and vitellogenesis related genes, including vitellogenins (vtg1, vtg2, zona pellucida sperm-binding proteins (zp3, zp4, and estrogen receptors (erα, erβ, with increases in plasma 17β-estradiol (E2 and vitellogenin (VTG concentrations. Following BaP treatment, detoxification- and biotransformation-related genes such as cyp1a1 and UDP-glucuronosyltransferase (ugt1a1 were significantly increased, with an increase in EROD activity. In both transcriptomes, mRNA expression of genes involved in antioxidant defense systems was increased, while genes involved in innate immunity were decreased upon BPS or BaP exposure with a decrease in complement activity. This study provides useful insight into the chemical-specific hepatic transcriptional response of P. olivaceus and suggests a basis for further studies examining biomarker application of liver transcriptomes for environmental pollution.

  12. Nuclei fluorescence microscopic observation on early embryonic development of mitogynogenetic diploid induced by hydrostatic pressure treatment in olive flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Lin, Zhengmei; Zhu, Xiangping; You, Feng; Wu, Zhihao; Cao, Yuanshui

    2015-05-01

    Sperm genetic material of olive flounder (Paralichthys olivaceus) was inactivated by ultraviolet irradiation. The nuclear phase changes during early embryonic development of diploid, haploid, and mitogynogenetic diploid induced by hydrostatic pressure treatment were observed under fluorescent microscope with 4',6-diamidino-2-phenylindole staining. The parameters of hydrostatic pressure treatment were 600 kg/cm(2) for 6 minutes at prometaphase stage. The data showed that developmental timing sequence of diploid and haploid fertilized eggs was similar. The cell cycle was about 48 minutes, including interphase (about 21 minutes), prophase (about 3 minutes), prometaphase (about 6 minutes), metaphase (about 6 minutes), anaphase (around 9 minutes), and telophase (about 3 minutes). After entering the fertilized egg, ultraviolet-inactivated sperm formed a male pronucleus and became a dense chromatin body in the cytoplasm. Dense chromatin body did not participate in nuclear division and unchanged all the time. For hydrostatic pressure-treated embryos, the first nuclear division and cytokinesis after treatment proceeded normally after about 15 minutes recovery. During the second mitosis, having undergone interphase, prophase, and prometaphase stage, chromosomes began to slowly spread around and scattered in the cell but not entered into metaphase and anaphase. The second nuclear division and cytokinesis was inhibited. The occurrence frequency of developmentally delayed embryos also showed that the second cleavage of about 80% treated eggs was inhibited. The inhibition of the second cleavage resulted to chromosome set doubling. So chromosome set doubling for mitogynogenetic flounder diploid induced by hydrostatic pressure treatment, performed at prometaphase stage, was mainly due to inhibition of the second mitosis rather than the first one. Copyright © 2015 Elsevier Inc. All rights reserved.

  13. Ultrastructural Observation of the Skin Chloride Cells of Japanese Flounder Paralichthys olivaceus and Turbot Scophthamus maximus Larvae

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The ultrastructures of skin chloride cells in cultured Japanese flounder and turbot larvae in metamorphosis, which grow in the same feeding conditions, are examined with a transmission electron microscope. These developed skin chloride cells were shaped like flattened ellipsoids and similar in morphology and ultrastructure to typical chloride cells of euryhaline fish gill. They locate in the epidermis and contract with the extra and interior environment through the apical pit and narrow channels. The cytoplasm of cell is full of numerous mitochondria and a ramifying network of tubules. The degeneration of skin chloride cells is observed with development of Japanese flounder larvae. Skin chloride cells of turbot are less developmental than those of Japanese flounder in the same developmental stage.

  14. Characterization of a specific monoclonal antibody against immunoglobulin light kappa/L1 chain in olive flounder (Paralichthys olivaceus)

    DEFF Research Database (Denmark)

    Kim, Young Kyu; Lee, Jung Seok; Jung, Jae Wook

    2017-01-01

    Immunoglobulins (Ig) are heterodimeric proteins that play critical roles in the adaptive immune system of vertebrates. Because of their plasticity, teleostean Igs are more diverse, and thus do not conform to mammalian classifications. Because of this, mammalian-based Ig cell markers cannot be used...... successfully to study immune responses in fish. There is therefore a need to produce Ig-specific cell markers for fish. Here, we attempted to identify the specific isotype detected by an Ig light chain-specific monoclonal antibody (anti-olive flounder IgL-mAb: M7C3-4) that we had previously produced [11......]. Three newly identified sequences of the Ig light chain from olive flounder were classified according to their isotypes. Subsequent analyses revealed that M7C3-4 was able to specifically detect lymphocytes expressing one of the κ chains (Igκ-a) in olive flounder. Interestingly, Igκ-a+ B cells were more...

  15. Evaluation of the inflammatory response to Kudoa septempunctata genotype ST3 isolated from olive flounder (Paralichthys olivaceus in Caco-2 cells

    Directory of Open Access Journals (Sweden)

    Ahn Meejung

    2018-01-01

    Full Text Available Kudoa septempunctata (Myxosporea, Multivalvulida is a parasite of the trunk muscle of cultured olive flounder (Paralichthys olivaceus. We investigated whether K. septempunctata genotype ST3 spores induce cell damage and the secretion of inflammatory mediators in Caco-2 cells, which exhibit characteristics similar to human intestinal epithelial cells. Purified K. septempunctata spores were heated at 95 °C for 5 min. Lactate dehydrogenase (LDH release was measured to determine the efficacy of denaturation. Naïve and heated spores, lipopolysaccharide (positive control and vehicle (negative control were added to Caco-2 cells. Cells were subjected to the cytotoxic LDH assay and western blot analysis to examine the expression of inducible nitric oxide synthase (iNOS and cyclooxygenase (COX-2. Supernatants were collected to measure nitric oxide (NO and prostaglandin E2 (PGE2. Most spores were denaturated by heating, and the spore morphology was found to be wrinkled with shell valves and polar capsules. In addition, cytotoxicity and inflammatory mediators, such as NO, PGE2, iNOS, and COX-2, remained unchanged in Caco-2 cells following exposure to naïve and heated spores compared with the positive controls. Collectively, the findings of this study imply that spores of K. septempunctata genotype ST3 do not cause inflammation in Caco-2 cells.

  16. Effects of waterborne nitrite on hematological parameters and stress indicators in olive flounders, Paralichthys olivaceus, raised in bio-floc and seawater.

    Science.gov (United States)

    Kim, Jun-Hwan; Kim, Jin-Young; Lim, Lok-Ji; Kim, Su Kyoung; Choi, Hye Sung; Hur, Young Baek

    2018-06-11

    Juvenile olive flounders, Paralichthys olivaceus (mean weight 2.69 ± 0.31 g), were raised in bio-floc and seawater for six months, these P. olivaceus (mean weight 280.1 ± 10.5 g, mean length 28.37 ± 2.3 cm) were exposed to different concentrations of waterborne nitrite (0, 25, 50, 100, and 200 mg NO 2 - /L) for 7 days. None of the P. olivaceus individuals exposed to bio-floc and seawater containing waterborne nitrite concentrations of 200 mg/L for 7 days survived. Hematological parameters (hemoglobin and hematocrit) were significantly reduced by nitrite exposure. Regarding plasma components, the concentrations of glucose, glutamic oxalate transaminase (GOT), and glutamic pyruvate transaminase (GPT) increased significantly in response to nitrite exposure, whereas cholesterol concentrations significantly decreased. Stress indicators, including concentrations of plasma glucose, cortisol, and liver and gill concentrations of heat shock protein 70 (HSP70) were significantly increased by nitrite exposure. The results of the study indicate that nitrite exposure affected the hematological parameters and stress indicators of P. olivaceus raised in bio-floc and seawater, and these changes were more prominent in the P. olivaceus raised in seawater than those raised in bio-floc. Copyright © 2018 Elsevier Ltd. All rights reserved.

  17. Immune response of flounder (Paralichthys olivaceus) was associated with the concentration of inactivated Edwardsiella tarda and immersion time.

    Science.gov (United States)

    Du, Yang; Tang, Xiaoqian; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin

    2015-09-15

    In the optimization of immersion strategy, vaccine concentration and immersion time are two key factors needed to be considered, which largely determined the immune efficacy. In this work, the healthy flounder were vaccinated in formalin-killed Edwardsiella tarda at four concentrations (10(6), 10(7), 10(8), 10(9) CFU ml(-1)) for three immersion times (30, 60, 90 min), respectively. At the 6th week post vaccination, the flounders were challenged with live E. tarda, and the relative percent survival (RPS) of flounder in 10(9)-30, 10(8)-60, 10(8)-90 and 10(7)-90 min groups were 70%, 78%, 74% and 65%, respectively, which were much higher than the other vaccination groups. Meanwhile, the sIg(+) cells in the leucocytes of peripheral blood (PBL), spleen (SL), head kidney (HKL) were monitored by flow cytometry, and the specific sera and mucosal antibodies were measured by indirect ELISA for 6 weeks. The results showed that the proportions of sIg(+) cells in PBL, SL and HKL of vaccinated fish were significantly higher than the untreated fish since the 2nd week (P0.05). These results demonstrated that the higher RPS was closely associated with stronger immune response, and immersion with formalin-inactivated E. tarda under 10(8) CFU ml(-1) for 60 min induced the highest immune response of flounder against E. tarda bacterin, which might be applied for the control of edwardsiellosis in flounder. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. INFLUENCE OF DIETARY PHYTIC ACID AND PHYTASE ON GROWTH, DIGESTIBILITY, AND VERTEBRAL PHOSPHORUS OF JUVENILE JAPANESE FLOUNDER, Paralichthys olivaceus

    Directory of Open Access Journals (Sweden)

    Asda Laining

    2013-06-01

    Full Text Available Triplicate groups of juvenile Japanese flounder (0.56 g were fed with six experimental diets to examine the effects of phytic acid, with or without phytase on growth performances, nutrient digestibility, and vertebral phosphorus (P content. Diet without both phytic acid (PA and phytase supplementation was used as control. One diet was added with 10 g PA/kg without phytase supplement. Four diets were formulated to contain two levels of phytase (1,000 FTU or 2,000 FTU phytase/kg diet combined with 2 levels of PA (10 and 20 g/kg diet. All diets were added with 10 g/kg in-organic P to meet flounder requirement. After 50 days culture, fish fed 10PA/2,000P grew significantly (P0.05 effects on feed intake and FCR. However, fish fed 10 g PA/kg combined with phytase had significant (P<0.05 higher whole body lipid, ash, and P than other groups. Moreover, P digestibility and vertebral P content were significantly increased by dietary phytase. This finding suggested that dietary phytase had potential to enhance the growth and nutrient utilization in juvenile Japanese flounder fed diet containing phytic acid. Specifically, inclusion of 2,000 FTU phytase/kg diet gave better performances when diet containing PA at level of 10 g/kg diet.

  19. Identification of immunogenic proteins and evaluation of four recombinant proteins as potential vaccine antigens from Vibrio anguillarum in flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Xing, Jing; Xu, Hongsen; Wang, Yang; Tang, Xiaoqian; Sheng, Xiuzhen; Zhan, Wenbin

    2017-05-31

    Vibrio anguillarum is a severe bacterial pathogen that can infect a wide range of fish species. Identification of immunogenic proteins and development of vaccine are essential for disease prevention. In this study, immunogenic proteins were screened and identified from V. anguillarum, and then protective efficacy of the immunogenic proteins was evaluated. Immunogenic proteins in V. anguillarum whole cell were detected by Western blotting (WB) using immunized flounder (Paralichthys olivaceus) serum, and then identified by Mass spectrometry (MS). The recombinant proteins of four identified immunogenic proteins were produced and immunized to fish, and then percentages of surface membrane immunoglobulin-positive (sIg+) cells in peripheral blood lymphocytes (PBL), total antibodies, antibodies against V. anguillarum, antibodies against recombinant proteins and relative percent survival (RPS) were measured, respectively. The results showed that five immunogenic proteins, VAA, Groel, OmpU, PteF and SpK, were identified; their recombinant proteins, rOmpU, rGroel, rSpK and rVAA, could induce the proliferation of sIg+ cells in PBL and production of total antibodies, antibodies against V. anguillarum and antibodies against the recombinant proteins; their protection against V. anguillarum showed 64.86%, 72.97%, 21.62% and 78.38% RPS, respectively. The results revealed that the immunoproteomic technique using fish anti-V. anguillarum serum provided an efficient way to screen the immunogenic protein for vaccine antigen. Moreover, the rVAA, rGroel and rOmpU had potential to be vaccine candidates against V. anguillarum infection. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. A multiple endpoint analysis of the effects of chronic exposure to sediment contaminated with Deepwater Horizon oil on juvenile Southern flounder and their associated microbiomes

    International Nuclear Information System (INIS)

    Brown-Peterson, Nancy J.; Krasnec, Michelle; Takeshita, Ryan; Ryan, Caitlin N.; Griffitt, Kimberly J.; Lay, Claire; Mayer, Gregory D.; Bayha, Keith M.; Hawkins, William E.; Lipton, Ian; Morris, Jeffrey

    2015-01-01

    Highlights: • Juvenile southern flounder were exposed to sediment mixed with different amount of oil from the Deepwater Horizon spill. • The exposure was performed for 32 days, with growth and survival assessed throughout. • After the termination of the experiment, the survivors were examined at multiple endpoints, including histopathology and microbiome analysis. • The results indicated that the flounder were adversely affected at each endpoint examined. • Growth and survival were significantly reduced. • Histopathology found effects on gills and livers of exposed fish. • The microbiomes of the exposed fish were significantly altered by the exposure to sediment-associated oil in both gills and intestines. - Abstract: Exposure to oiled sediments can negatively impact the health of fish species. Here, we examine the effects of chronic exposure of juvenile southern flounder, Paralichthys lethostigma, to a sediment-oil mixture. Oil:sediment mixtures are persistent over time and can become bioavailable following sediment perturbation or resuspension. Juvenile flounder were exposed for 32 days under controlled laboratory conditions to five concentrations of naturally weathered Macondo MC252 oil mixed into uncontaminated, field-collected sediments. The percent composition of individual polycyclic aromatic hydrocarbons (PAHs) of the weathered oil did not change after mixing with the sediment. Spiked exposure sediments contained 0.04–395 mg/kg tPAH50 (sum of 50 individual PAH concentration measurements). Mortality increased with both exposure duration and concentration of sediment-associated PAHs, and flounder exposed to concentrations above 8 mg/kg tPAH50 showed significantly reduced growth over the course of the experiment. Evident histopathologic changes were observed in liver and gill tissues of fish exposed to more than 8 mg/kg tPAH50. All fish at these concentrations showed hepatic intravascular congestion, macrovesicular hepatic vacoulation

  1. A multiple endpoint analysis of the effects of chronic exposure to sediment contaminated with Deepwater Horizon oil on juvenile Southern flounder and their associated microbiomes

    Energy Technology Data Exchange (ETDEWEB)

    Brown-Peterson, Nancy J., E-mail: nancy.brown-peterson@usm.edu [Department of Coastal Sciences, The University of Southern Mississippi, 703 East Beach Dr., Ocean Springs, MS 39564 (United States); Krasnec, Michelle, E-mail: MKrasnec@stratusconsulting.com [Abt Associates, 1881 Ninth Street, Suite 201, Boulder, Colorado 80302 (United States); Takeshita, Ryan, E-mail: RTakeshita@stratusconsulting.com [Abt Associates, 1881 Ninth Street, Suite 201, Boulder, Colorado 80302 (United States); Ryan, Caitlin N., E-mail: Caitlin.ryan@ttu.edu [The Institute of Environmental and Human Health, Department of Environmental Toxicology, Texas Tech University, Box 41163, Lubbock, TX 79409 (United States); Griffitt, Kimberly J., E-mail: kim.griffitt@gmail.com [Department of Coastal Sciences, The University of Southern Mississippi, 703 East Beach Dr., Ocean Springs, MS 39564 (United States); Lay, Claire, E-mail: claymsc@stratusconsulting.com [Abt Associates, 1881 Ninth Street, Suite 201, Boulder, Colorado 80302 (United States); Mayer, Gregory D., E-mail: greg.mayer@ttu.edu [The Institute of Environmental and Human Health, Department of Environmental Toxicology, Texas Tech University, Box 41163, Lubbock, TX 79409 (United States); Bayha, Keith M., E-mail: kmbayha@gmail.com [Department of Coastal Sciences, The University of Southern Mississippi, 703 East Beach Dr., Ocean Springs, MS 39564 (United States); Hawkins, William E., E-mail: william.hawkins@usm.edu [Department of Coastal Sciences, The University of Southern Mississippi, 703 East Beach Dr., Ocean Springs, MS 39564 (United States); Lipton, Ian, E-mail: ianlipton@stratusconsulting.com [Abt Associates, 1881 Ninth Street, Suite 201, Boulder, Colorado 80302 (United States); Morris, Jeffrey, E-mail: JMorrisMSCanyon252@stratusconsulting.com [Abt Associates, 1881 Ninth Street, Suite 201, Boulder, Colorado 80302 (United States); and others

    2015-08-15

    Highlights: • Juvenile southern flounder were exposed to sediment mixed with different amount of oil from the Deepwater Horizon spill. • The exposure was performed for 32 days, with growth and survival assessed throughout. • After the termination of the experiment, the survivors were examined at multiple endpoints, including histopathology and microbiome analysis. • The results indicated that the flounder were adversely affected at each endpoint examined. • Growth and survival were significantly reduced. • Histopathology found effects on gills and livers of exposed fish. • The microbiomes of the exposed fish were significantly altered by the exposure to sediment-associated oil in both gills and intestines. - Abstract: Exposure to oiled sediments can negatively impact the health of fish species. Here, we examine the effects of chronic exposure of juvenile southern flounder, Paralichthys lethostigma, to a sediment-oil mixture. Oil:sediment mixtures are persistent over time and can become bioavailable following sediment perturbation or resuspension. Juvenile flounder were exposed for 32 days under controlled laboratory conditions to five concentrations of naturally weathered Macondo MC252 oil mixed into uncontaminated, field-collected sediments. The percent composition of individual polycyclic aromatic hydrocarbons (PAHs) of the weathered oil did not change after mixing with the sediment. Spiked exposure sediments contained 0.04–395 mg/kg tPAH50 (sum of 50 individual PAH concentration measurements). Mortality increased with both exposure duration and concentration of sediment-associated PAHs, and flounder exposed to concentrations above 8 mg/kg tPAH50 showed significantly reduced growth over the course of the experiment. Evident histopathologic changes were observed in liver and gill tissues of fish exposed to more than 8 mg/kg tPAH50. All fish at these concentrations showed hepatic intravascular congestion, macrovesicular hepatic vacoulation

  2. Effects of polychlorinated biphenyls on metamorphosis of a marine fish Japanese flounder (Paralichthys olivaceus) in relation to thyroid disruption.

    Science.gov (United States)

    Dong, Yifei; Zhang, Xiaona; Tian, Hua; Li, Xiang; Wang, Wei; Ru, Shaoguo

    2017-06-15

    This study examined the influence of environmental concentrations of Aroclor 1254 (10, 100, and 1000ng/L) on metamorphosis of Paralichthys olivaceus, and analyzed the mechanisms in relation to thyroid disruption. Results showed that 100 and 1000ng/L Aroclor 1254 delayed metamorphosis and that 1000ng/L Aroclor 1254 caused abnormal morphology. Thyroxine and triiodothyronine levels in the control group were significantly elevated at metamorphic climax, but treatment with 100 and 1000ng/L delayed the increase in thyroid hormones (THs) and retarded metamorphic processes. In larvae exposed to 1000ng/L Aroclor 1254, TH levels at metamorphic climax were significantly lower than those of the control group at the same metamorphic stage. We suggest that the effects of Aroclor 1254 on larval metamorphosis can be explained by disruption of thyroid homeostasis. These findings provide a new perspective and biological model for thyroid-disrupting chemicals (TDCs) screening and investigating interference of thyroid function by TDCs. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. FLUCTUATION OF POST-PRANDIAL PLASMA MINERAL LEVEL OF JUVENILE JAPANESE FLOUNDER, Paralichthys olivaceus FED DIETARY PHOSPHORUS AND PHYTASE SUPPLEMENTATION

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    Asda Laining

    2010-12-01

    Full Text Available In order to investigate the phytic acid degradation in the gut of post juvenile Japanese flounder, indirect method was carried out by measuring the pre-prandial and postprandial plasma mineral and alkaline phosphatase (ALP level as well as liver phosphorus content. The experiment was designed into a Randomized Block in which experiment units were grouped according to sampling days at 10, 20 and 30 days of feeding time. Experimental diets contained three levels of dietary inorganic phosphorus at 0.0; 0.25 and 0.5% combined with two levels of dietary phytase at 0 and 2,000 FTU/kg diet. Juvenile Japanese flounder (IBW = 36.2 g were randomly distributed into 6 tanks of a 200 L capacity with density of 15 fish/tank. Blood sampling was carried out at 0 hour (before feeding or pre-prandial and at 1, 3, 6 and 12 hour post feeding (post-prandial time in three sampling days, respectively. Plasma was measured for mineral and ALP levels, while liver was analyzed for P content. The observation showed that fish fed without both dietary IP and phytase supplements had the lowest postprandial plasma IP, Mg and ALP levels during 12-h postprandial period. Plasma IP level at 6-h post-feeding in groups fed dietary 0.25 and 0.5% IP were significant higher when diet supplemented with phytase than those without phytase supplement. Peak level of plasma IP in fish fed 0.25% IP was similar to fish fed 0.5% with the presence of dietary phytase. At 1 and 3-h post-feeding, plasma Ca level increased in all groups, but significant difference was only observed between group fed diet without both dietary IP and phytase and other groups. Similar to plasma IP level, peak of plasma Mg and ALP concentration occurred in fish fed 0.25% IP together with phytase, and did not significantly differ from fish fed with 0.5% IP even when phytase was included in diet.

  4. Insight into the heat resistance of fish via blood: Effects of heat stress on metabolism, oxidative stress and antioxidant response of olive flounder Paralichthys olivaceus and turbot Scophthalmus maximus.

    Science.gov (United States)

    Lu, Yunliang; Wu, Zhihao; Song, Zongcheng; Xiao, Peng; Liu, Ying; Zhang, Peijun; You, Feng

    2016-11-01

    High temperature has direct confinement on fish survival and growth, especially under the background of global warming. Selection of fish line with heat resistance is an important means to address this problem. In the present study, we analyzed the difference in heat resistance between families of olive flounder Paralichthys olivaceus and turbot Scophthalmus maximus, two flatfish species occupying slightly different thermal niches. Then the chosen families were tested to determine their differential response to heat stress (ΔT = +8 °C and +12 °C) in blood, including anaerobic metabolism (lactate), oxidative stress (lipid peroxidation and protein carbonylation) and antioxidant enzymes. Results showed a difference in heat resistance between families of the two species. Among the chosen parameters, growth traits had a significant effect on contents of lactate and malondialdehyd (MDA), and activities of catalase (CAT) and glutathione S-transferase in flounder (P heat-sensitive family of each species, levels of all studied parameters were lower and more stable in heat-resistant families after heat stress. What's more, heat resistance of fish significantly influenced contents of lactate and MDA and activity of CAT in flounder (P heat-resistance, being potentially valuable in fish breeding. However, these markers should be applied with more caution when there is a growth discrepancy between fish families. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. Salinity mediates the toxic effect of nano-TiO2 on the juvenile olive flounder Paralichthys olivaceus.

    Science.gov (United States)

    Huang, Xizhi; Lan, Yawen; Liu, Zekang; Huang, Wei; Guo, Qindan; Liu, Liping; Hu, Menghong; Sui, Yanming; Wu, Fangli; Lu, Weiqun; Wang, Youji

    2018-06-04

    Increased production of engineered nanoparticles has raised extensive concern about the potential toxic effects on marine organisms living in estuarine and coastal environments. Meanwhile, salinity is one of the key environmental factors that may influence the physiological activities in flatfish species inhabiting in those waters due to fluctuations caused by freshwater input or rainfall. In this study, we investigated the oxidative stress and histopathological alteration of the juvenile Paralichthys olivaceus exposed to nano-TiO 2 (1 and 10 mg L -1 ) under salinities of 10 and 30 psu for 4 days. In the gills, Na + -K + -ATPase activity significantly deceased after 4 days 10 psu exposure without nano-TiO 2 compared with 1 day of acclimating the salinity from the normal salinity (30 psu) to 10 psu. Under this coastal salinity, low concentration (1 mg L -1 ) of nano-TiO 2 exerted significant impacts. In the liver, the activities of superoxide dismutase, catalase, the levels of lipid peroxide and malondialdehyde increased with nano-TiO 2 exposed under 30 psu. Such increase indicated an oxidative stress response. The result of the integrated biomarker responses showed that P. olivaceus can be adversely affected by high salinity and high concentration of nano-TiO 2 for a short-term (4 days) exposure. The histological analysis revealed the accompanying severe damages for the gill filaments. Principal component analysis further showed that the oxidative stress was associated with the nano-TiO 2 effect at normal salinity. These findings indicated that nano-TiO 2 and normal salinity exert synergistic effects on juvenile P. olivaceus, and low salinity plays a protective role in its physiological state upon short-term exposure to nano-TiO 2 . The mechanism of salinity mediating the toxic effects of NPs on estuarine fish should be further considered. Copyright © 2018 Elsevier B.V. All rights reserved.

  6. The integrated analysis of RNA-seq and microRNA-seq depicts miRNA-mRNA networks involved in Japanese flounder (Paralichthys olivaceus) albinism.

    Science.gov (United States)

    Wang, Na; Wang, Ruoqing; Wang, Renkai; Tian, Yongsheng; Shao, Changwei; Jia, Xiaodong; Chen, Songlin

    2017-01-01

    Albinism, a phenomenon characterized by pigmentation deficiency on the ocular side of Japanese flounder (Paralichthys olivaceus), has caused significant damage. Limited mRNA and microRNA (miRNA) information is available on fish pigmentation deficiency. In this study, a high-throughput sequencing strategy was employed to identify the mRNA and miRNAs involved in P. olivaceus albinism. Based on P. olivaceus genome, RNA-seq identified 21,787 know genes and 711 new genes by transcripts assembly. Of those, 235 genes exhibited significantly different expression pattern (fold change ≥2 or ≤0.5 and q-value≤0.05), including 194 down-regulated genes and 41 up-regulated genes in albino versus normally pigmented individuals. These genes were enriched to 81 GO terms and 9 KEGG pathways (p≤0.05). Among those, the pigmentation related pathways-Melanogenesis and tyrosine metabolism were contained. High-throughput miRNA sequencing identified a total of 475 miRNAs, including 64 novel miRNAs. Furthermore, 33 differentially expressed miRNAs containing 13 up-regulated and 20 down-regulated miRNAs were identified in albino versus normally pigmented individuals (fold change ≥1.5 or ≤0.67 and p≤0.05). The next target prediction discovered a variety of putative target genes, of which, 134 genes including Tyrosinase (TYR), Tyrosinase-related protein 1 (TYRP1), Microphthalmia-associated transcription factor (MITF) were overlapped with differentially expressed genes derived from RNA-seq. These target genes were significantly enriched to 254 GO terms and 103 KEGG pathways (p<0.001). Of those, tyrosine metabolism, lysosomes, phototransduction pathways, etc., attracted considerable attention due to their involvement in regulating skin pigmentation. Expression patterns of differentially expressed mRNA and miRNAs were validated in 10 mRNA and 10 miRNAs by qRT-PCR. With high-throughput mRNA and miRNA sequencing and analysis, a series of interested mRNA and miRNAs involved in fish

  7. INTERACTION BETWEEN DIETARY MINERAL AND PHYTASE ON BIOLOGICAL PERFORMANCES OF JAPANESE FLOUNDER, Paralichthys olivaceus. PART II. MINERAL DIGESTIBILITY AND VERTEBRAL MINERAL CONTENT

    Directory of Open Access Journals (Sweden)

    Asda Laining

    2015-06-01

    Full Text Available Interactive effects between dietary inorganic phosphorus (IP and phytase (P on mineral digestibility and vertebral mineral content were investigated in a 30 days feeding trial followed by three weeks digestibility trial with Japanese flounder, Paralichthys olivaceus. Eight experimental diets were formulated based on two levels of dietary Ca at 0% and 0.2% combined with either 0% or 0.25% of dietary IP and either with 0 and 2,000 fytase unit (FTU/kg of phytase in diet, respectively. Result indicated that digestibility of total phosphorus significantly increased by three dietary compounds where the highest was observed in fish fed diet contained 0.25% IP and 2,000 FTU phytase/kg and dietary Ca also included in diet. Significant interaction was only detected between dietary IP and P on this parameter. Supplementation of IP and Ca not phytase significantly improved Ca digestibility. Ca digestibility was very poor when dietary IP and Ca were not supplemented in diet even with when phytase supplemented in diet. There was significant interaction between dietary IP and Ca on Ca digestibility. Vertebral total phosphorus, Ca, and Mg content as well as Ca:P ratio were significantly enhanced by dietary IP and phytase. Dietary Ca has significant effect only on vertebral total phosphorus. Interaction between dietary IP and Ca was significantly found on vertebral Ca content and Ca:P ratio. No significant second-order interaction was observed among the three dietary mineral on overall parameters. Based on total phosphorus and Ca digestibility as well vertebral phosphorus content found in this study, dietary IP, Ca, and phytase at rate of 0.25%, 0.2%, and 2,000 FTU phytase/kg diet, respectively are needed to supplement in diet for a better mineral absorption and bone mineralization.

  8. Protective efficacy of six immunogenic recombinant proteins of Vibrio anguillarum and evaluation them as vaccine candidate for flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Xing, Jing; Xu, Hongsen; Wang, Yang; Tang, Xiaoqian; Sheng, Xiuzhen; Zhan, Wenbin

    2017-06-01

    Vibrio anguillarum is a severe bacterium that causes terminal haemorrhagic septicaemia in freshwater and marine fish. Virulence-associated proteins play an important role in bacterial pathogenicity and could be applied for immunoprophylaxis. In this study, six antigenic proteins from V. anguillarum were selected and the immune protective efficacy of their recombinant proteins was investigated. VirA, CheR, FlaC, OmpK, OmpR and Hsp33 were recombinantly produced and the reactions of recombinant proteins to flounder-anti-V. anguillarum antibodies (fV-ab) were detected, respectively. Then the recombinant proteins were injected to fish, after immunization, the percentages of surface membrane immunoglobulin-positive (sIg+) cell in lymphocytes, total antibodies, antibodies against V. anguillarum, antibodies against recombinant proteins and relative percent survival (RPS) were analyzed, respectively. The results showed that all the recombinant proteins could react to fV-ab, proliferate sIg + cells in lymphocytes and induce production of total antibodies, specific antibodies against V. anguillarum or the recombinant proteins; the RPS of rVirA, rCheR, rFlaC, rOmpK, rOmpR and rHsp33 against V. anguillarum was 70.27%, 27.03%, 16.22%, 62.16%, 45.95% and 81.08%, respectively. The results revealed that rHsp33, rVirA and rOmpK have good protections against V. anguillarum and could be vaccine candidates against V. anguillarum. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. Population Subdivision of Japanese Flounder Paralichthys olivaceus in the Pacific Coast of Tohoku Japan Detected by Means of Mitochondrial Phylogenetic Information

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    Yuya Shigenobu

    2013-01-01

    Full Text Available This study deals with mitochondrial phylogenetic information of Japanese flounder in the Pacific coast of Tohoku Japan to estimate the genetic population subdivision that was undetectable by conventional population statistics. We determined complete sequences of mitochondrial NADH dehydrogenase subunit-2 (ND2 and subunit-5 (ND5 genes for 151 individuals from northern (Aomori and Iwate prefectures, 40–41°N and southern (Miyagi and Fukushima prefectures, 37–38°N waters. Samples from both waters showed high genetic diversity, including 126 haplotypes. These haplotypes were located at mixed and nested positions on an inferred phylogenetic tree, and traditional F-statistics indicated no significant population divergence (φST = −0.00335, p > 0.05, corroborating our previous study. Three variable sites, however, showed significant base composition heterogeneity between samples from the northern and southern waters (Fisher’s exact-test, p < 0.01. Nucleotide substitutions at the three sites converged on an apical clade, which consisted of the five southern individuals, whereas its sister clade consisted only of the three northern individuals. This phylogenetic information corroborates previous ecological studies indicating the presence of separate stocks in the northern and southern waters.

  10. The effects of IL-1β, IL-8, G-CSF and TNF-α as molecular adjuvant on the immune response to an E. tarda subunit vaccine in flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Guo, Ming; Tang, Xiaoqian; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin

    2018-06-01

    Cytokines play vital roles in mounting immune responses and activating host defense network. In this study, the expression plasmid pcDNA3.1 (pcN3) encoding four flounder (Paralichthys olivaceus) cytokines including IL-1β, TNF-α, IL-8 or G-CSF (pcIL-1β, pcTNF-α, pcIL-8 and pcG-CSF) were successfully constructed, and their adjuvant potential on an Edwardsiella tarda (E. tarda) subunit vaccine OmpV (rOmpV) were comparatively analyzed in vaccinated flounder model. Results revealed that flounder vaccinated with rOmpV plus pcIL-1β, pcIL-8 or pcG-CSF produced the relative percent survivals (RPS) of 71%, 65% and 49% respectively, which were higher than that in flounder vaccinated with rOmpV plus pcTNF-α (39%) or pcN3 (36%, the control group). Immunological analysis showed that: (1) except pcTNF-α, higher levels of anti-E. tarda serum antibodies and sIg + lymphocytes in spleen, head kidney and peripheral blood were significantly enhanced by pcIL-1β, pcIL-8 or pcG-CSF, however, pcIL-8 and pcIL-1β enhanced higher levels of sIg + lymphocytes and anti-E. tarda antibodies than pcG-CSF; (2) pcTNF-α could promote the up-regulation of genes participated in cellular immunity (MHCIα, IFN-γ, CD8α and CD8β), pcIL-1β could enhance the expression of genes related to humoral immunity (CD4-1, CD4-2, MHCIIα and IgM), and all the detected genes were augmented by pcIL-8 and pcG-CSF; Among the four cytokines, pcIL-8 and pcIL-1β could strengthen the highest levels of genes participated in cellular immunity and humoral immunity, respectively. These results demonstrated that pcIL-8 and pcIL-1β could enhance stronger cellular and/or humoral immunity induced by rOmpV than pcG-CSF and pcTNF-α, and evoked higher RPS against E. tarda challenge in flounder, which indicated that pcIL-8 and pcIL-1β are promising adjuvants of vaccines in controlling E. tarda infection. Copyright © 2018 Elsevier Ltd. All rights reserved.

  11. A Lactococcus lactis BFE920 feed vaccine expressing a fusion protein composed of the OmpA and FlgD antigens from Edwardsiella tarda was significantly better at protecting olive flounder (Paralichthys olivaceus) from edwardsiellosis than single antigen vaccines.

    Science.gov (United States)

    Beck, Bo Ram; Lee, Soon Ho; Kim, Daniel; Park, Ji Hye; Lee, Hyun Kyung; Kwon, San-Sung; Lee, Kwan Hee; Lee, Jae Il; Song, Seong Kyu

    2017-09-01

    Edwardsiellosis is a major fish disease that causes a significant economic damage in the aquaculture industry. Here, we assessed vaccine efficacy after feeding oral vaccines to olive flounder (Paralichthys olivaceus), either L. lactis BFE920 expressing Edwardsiella tarda outer membrane protein A (OmpA), flagellar hook protein D (FlgD), or a fusion antigen of the two. Feed vaccination was done twice with a one-week interval. Fish were fed regular feed adsorbed with the vaccines. Feed vaccination was given over the course of one week to maximize the interaction between the feed vaccines and the fish intestine. Flounder fed the vaccine containing the fusion antigen had significantly elevated levels T cell genes (CD4-1, CD4-2, and CD8α), type 1 helper T cell (Th1) subset indicator genes (T-bet and IFN-γ), and antigen-specific antibodies compared to the groups fed the single antigen-expressing vaccines. Furthermore, the superiority of the fusion vaccine was also observed in survival rates when fish were challenged with E. tarda: OmpA-FlgD-expressing vaccine (82.5% survival); FlgD-vaccine (55.0%); OmpA-vaccine (50%); WT L. lactis BFE920 (37.5%); Ctrl (10%). In addition, vaccine-fed fish exhibited increased weight gain (∼20%) and a decreased feed conversion ratio (∼20%) during the four week vaccination period. Flounder fed the FlgD-expressing vaccine, either the single or the fusion form, had significantly increased expression of TLR5M, IL-1β, and IL-12p40, suggesting that the FlgD may be a ligand of olive flounder TLR5M receptor or closely related to the TLR5M pathway. In conclusion, the present study demonstrated that olive flounder fed L. lactis BFE920 expressing a fusion antigen composed of E. tarda OmpA and FlgD showed a strong protective effect against edwardsiellosis indicating this may be developed as an E. tarda feed vaccine. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Pellet feed adsorbed with the recombinant Lactococcus lactis BFE920 expressing SiMA antigen induced strong recall vaccine effects against Streptococcus iniae infection in olive flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Kim, Daniel; Beck, Bo Ram; Lee, Sun Min; Jeon, Jongsu; Lee, Dong Wook; Lee, Jae Il; Song, Seong Kyu

    2016-08-01

    The aim of this study was to develop a fish feed vaccine that provides effective disease prevention and convenient application. A lactic acid bacterium (LAB), Lactococcus lactis BFE920, was modified to express the SiMA antigen, a membrane protein of Streptococcus iniae. The antigen was engineered to be expressed under the nisin promoter, which is induced by nisin produced naturally by the host LAB. Various sizes (40 ± 3.5 g, 80 ± 2.1 g, and 221 ± 2.4 g) of olive flounder (Paralichthys olivaceus) were vaccinated by feeding the extruded pellet feed, onto which the SiMA-expressing L. lactis BFE920 (1.0 × 10(7) CFU/g) was adsorbed. Vaccine-treated feed was administered twice a day for 1 week, and priming and boosting were performed with a 1-week interval in between. The vaccinated fish had significantly elevated levels of antigen-specific serum antibodies and T cell marker mRNAs: CD4-1, CD4-2, and CD8a. In addition, the feed vaccine significantly induced T cell effector functions, such as the production of IFN-γ and activation of the transcription factor that induces its expression, T-bet. When the flounder were challenged by intraperitoneal infection and bath immersion with S. iniae, the vaccinated fish showed 84% and 82% relative percent survival (RPS), respectively. Furthermore, similar protective effects were confirmed even 3 months after vaccination in a field study (n = 4800), indicating that this feed vaccine elicited prolonged duration of immunopotency. In addition, the vaccinated flounder gained 21% more weight and required 16% less feed to gain a unit of body weight compared to the control group. The data clearly demonstrate that the L. lactis BFE920-SiMA feed vaccine has strong protective effects, induces prolonged vaccine efficacy, and has probiotic effects. In addition, this LAB-based fish feed vaccine can be easily used to target many different pathogens of diverse fish species. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Evolutionary Conservation of pou5f3 Genomic Organization and Its Dynamic Distribution during Embryogenesis and in Adult Gonads in Japanese Flounder Paralichthys olivaceus

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    Jinning Gao

    2017-01-01

    Full Text Available Octamer-binding transcription factor 4 (Oct4 is a member of POU (Pit-Oct-Unc transcription factor family Class V that plays a crucial role in maintaining the pluripotency and self-renewal of stem cells. Though it has been deeply investigated in mammals, its lower vertebrate homologue, especially in the marine fish, is poorly studied. In this study, we isolated the full-length sequence of Paralichthys olivaceus pou5f3 (Popou5f3, and we found that it is homologous to mammalian Oct4. We identified two transcript variants with different lengths of 3′-untranslated regions (UTRs generated by alternative polyadenylation (APA. Quantitative real-time RT-PCR (qRT-PCR, in situ hybridization (ISH and immunohistochemistry (IHC were implemented to characterize the spatial and temporal expression pattern of Popou5f3 during early development and in adult tissues. Our results show that Popou5f3 is maternally inherited, abundantly expressed at the blastula and early gastrula stages, then greatly diminishes at the end of gastrulation. It is hardly detectable from the heart-beating stage onward. We found that Popou5f3 expression is restricted to the adult gonads, and continuously expresses during oogenesis while its dynamics are downregulated during spermatogenesis. Additionally, numerous cis-regulatory elements (CRE on both sides of the flanking regions show potential roles in regulating the expression of Popou5f3. Taken together, these findings could further our understanding of the functions and evolution of pou5f3 in lower vertebrates, and also provides fundamental information for stem cell tracing and genetic manipulation in Paralichthys olivaceus.

  14. Plasma leptin and growth hormone levels in the fine flounder (Paralichthys adspersus) increase gradually during fasting and decline rapidly after refeeding.

    Science.gov (United States)

    Fuentes, Eduardo N; Kling, Peter; Einarsdottir, Ingibjörg Eir; Alvarez, Marco; Valdés, Juan Antonio; Molina, Alfredo; Björnsson, Björn Thrandur

    2012-05-15

    In fish, recent studies have indicated an anorexigenic role of leptin and thus its possible involvement in regulation of energy balance and growth. In the present study, the effects of fasting and refeeding periods on plasma leptin levels were studied in the fine flounder, a flatfish with remarkably slow growth. To further assess the endocrine status of the fish during periods of catabolism and anabolism, plasma growth hormone (GH) levels were also analyzed. Under normal feeding condition, plasma leptin and GH levels remained stable and relatively high in comparison with other teleost species. For the three separate groups of fish, fasted for 2, 3, and 4 weeks, respectively, plasma leptin levels increase gradually, becoming significantly elevated after 3 weeks, and reaching highest levels after 4-week fasting. Plasma GH levels were significantly elevated after 2-week fasting. At the onset of refeeding, following a single meal, leptin levels decline rapidly to lower than initial levels within 2 h, irrespective of the length of fasting. Plasma GH also decline, the decrease being significant after 4, 24 and 2 h for the 2, 3 and 4-week fasted groups, respectively. This study shows that plasma leptin levels in the fine flounder are strongly linked to nutritional status and suggests that leptin secretion is regulated by fast-acting mechanisms. Elevated leptin levels in fasted fish may contribute to a passive survival strategy of species which experience natural food shortage periods by lowering appetite and limiting physical foraging activity. Copyright © 2012 Elsevier Inc. All rights reserved.

  15. The influence of concentration of inactivated Edwardsiella tarda bacterin and immersion time on antigen uptake and expression of immune-related genes in Japanese flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Du, Yang; Tang, Xiaoqian; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin

    2017-02-01

    Our previous work has demonstrated that the immune response of Japanese flounder was associated with the concentration of formalin-inactivated Edwardsiella tarda and immersion time. In order to further investigate the influence of immersion vaccine dose and bath time on the antigen uptake, formalin-killed Edwardsiella tarda bacterin was prepared and adjusted to four concentrations (10 9 , 10 8 , 10 7 , 10 6  cfu ml -1 ) for 30, 60 and 90 min immersion in Japanese flounder model, respectively. Absolute quantitative real-time PCR was employed to examine the bacterin uptake in gill, skin, spleen and kidney at 3 and 6 h post vaccination. The results showed that the antigen uptaken in gills and skin were significant higher than spleen and kidney, and the antigen amounts in gill and skin both declined from 3 to 6 h, whereas the antigen amounts in spleen and kidney gradually increased. Significant higher antigen amounts were detected in 10 9 -30, 10 9 -60, 10 8 -60, 10 8 -90 and 10 8 -90 groups than other groups (P immersion with formalin-inactivated E. tarda, especially under 10 8 -60 min condition could efficiently enhance the antigen uptake and the expression of immune-related genes, which provided evidences for an enhanced vaccination effects under an optimized combination of vaccine dose and immersion time. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. Two skin cell lines from wild-type and albino Japanese flounder (Paralichthys olivaceus): establishment, characterization, virus susceptibility, efficient transfection, and application to albinism study.

    Science.gov (United States)

    Wang, Ruoqing; Zhang, Nianwei; Wang, Renkai; Wang, Shengpeng; Wang, Na

    2017-12-01

    In order to provide an applicable cell platform to study fish pathology and skin pigmentation, two cell lines derived from skin tissues of wild-type and albino Japanese flounder were established and named JFSK_wt and JFSK_alb, respectively. These two cell lines were cultured for 45 passages within approximately 300 days. JFSK_wt and JFSK_alb cells were maintained in Dulbecco's Modified Eagle's Medium and Ham's F-12 Nutrient Mixture (DMEM/F12) supplemented with antibiotics, fetal bovine serum (FBS), 2-mercaptoethanol (2-Me), N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES), and basic fibroblast growth factor (bFGF). The optimal growth temperature for JFSK_wt and JFSK_alb cells was 24 °C, and microscopically, the two cell lines were composed of fibroblast-like cells. Chromosomal analysis revealed that JFSK_wt and JFSK_alb cells had an identical diploid karyotype with 2n = 48t. Results of viral inoculation assays revealed that both cell lines shared similar patterns of viral susceptibility to nervous necrosis virus (NNV). High transfection efficiency was observed in JFSK_wt and JFSK_alb cells transfected with a pEGFP-N3 reporter plasmid and Cy3-siRNA. The detection of dermal marker Dermo-1 showed that these two cells were both derived from the dermis. Finally, three genes involved in the melanogenesis pathway, including adenylate cyclase type 5 (adcy5), microphthalmia-associated transcription factor (mitf), and endothelin B receptor (ednrb), were downregulated in JFSK_alb versus JFSK_wt cells. Thus, the two cell lines, sampled from skin tissue of wild-type and albino Japanese flounder will be not only helpful for fish pathogen research but also beneficial for albinism-related gene function studies.

  17. INTERACTION BETWEEN DIETARY MINERAL AND PHYTASE ON BIOLOGICAL PERFORMANCES OF JAPANESE FLOUNDER, Paralichthys olivaceus. PART I. GROWTH, FEED INTAKE, AND WHOLE BODY MINERAL CONTENT

    Directory of Open Access Journals (Sweden)

    Asda Laining

    2013-06-01

    Full Text Available In order to determine the effect of dietary calcium (Ca, inorganic phosphorus (IP, and phytase (P supplementation in marine fish, a 2 x 2 x 2 factorial design was arranged to observe the interrelationship between dietary Ca and IP with the presence of dietary phytase. Two levels of dietary Ca at 0% and 0.2% combined with either 0% or 0.25% of dietary IP and either with 0 and 2,000 FTU phytase/kg diet, respectively to formulate eight experimental diets. SPI-based diet was used as basal diet and the sources of Ca, IP, and phytase were similar to those used in the previous experiment. Juvenile Japanese flounder with initial body weight around 0.6 g was fed the test diets. After 30 days of feeding trial, the results showed that both dietary IP and phytase, but not dietary Ca significantly enhanced the growth and feed intake. The highest growth was achieved in fish fed a diet containing the Ca, IP, and phytase supplement among groups. Fish fed diet without the three dietary supplements had the lowest SGR and did not significantly improve by supplementing dietary Ca. Feed intake (FI and was significantly influenced by dietary IP and phytase, not dietary Ca while feed conversion ratio (FCR was significantly affected by all dietary treatments. Interaction effect was detected between dietary Ca and IP, between dietary IP and P on FCR. Total phosphorus content in whole body was significantly increased by supplementing all dietary treatments which was highest in fish fed 0.25 IP/0.2 Ca/P. Significant interaction was observed between dietary IP and P on this parameter. Whole body Ca content was significantly improved by either dietary IP or Ca and not dietary P. As conclusion even without inorganic Ca supplement, dietary IP at level of 0.25% or 2,000 FTU phytase/kg diet could enhance growth and FI of fish as well as whole body phosphorus content of juvenile Japanese flounder when diet basal contained organic Ca around 1.2%.

  18. A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)

    Science.gov (United States)

    Yang, Yang; Liu, Qinghua; Ma, Daoyuan; Song, Zongchen; Li, Jun

    2018-04-01

    Some germ cell marker genes, such as vasa, nanos, and dead end (dnd), have been identified in fish. Recently, lymphocyte antigen 75 (Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder ly75 homolog (ly75) was cloned and its expression pattern in gonads was analyzed. The full-length cDNA of ly75 was 7 346 bp, with an open reading frame (ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 kDa. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes.

  19. The DNA methylation status of MyoD and IGF-I genes are correlated with muscle growth during different developmental stages of Japanese flounder (Paralichthys olivaceus).

    Science.gov (United States)

    Huang, Yajuan; Wen, Haishen; Zhang, Meizhao; Hu, Nan; Si, Yufeng; Li, Siping; He, Feng

    2018-05-01

    Many genes related to muscle growth modulate myoblast proliferation and differentiation and promote muscle hypertrophy. MyoD is a myogenic determinant that contributes to myoblast determination, and insulin-like growth factor 1 (IGF-I) interacts with MyoD to regulate muscle hypertrophy and muscle mass. In this study, we aimed to assess DNA methylation and mRNA expression patterns of MyoD and IGF-I during different developmental stages of Japanese flounder, and to examine the relationship between MyoD and IGF-I gene. DNA and RNA were extracted from muscles, and DNA methylation of MyoD and IGF-I promoter and exons was detected by bisulfite sequencing. The relative expression of MyoD and IGF-I was measured by quantitative polymerase chain reaction. IGF-I was measured by radioimmunoassay. Interestingly, the lowest expression of MyoD and IGF-I emerged at larva stage, and the mRNA expression was negatively associated with methylation. We hypothesized that many skeletal muscle were required to complete metamorphosis; thus, the expression levels of MyoD and IGF-I genes increased from larva stage and then decreased. The relative expression levels of MyoD and IGF-I exhibited similar patterns, suggesting that MyoD and IGF-I regulated muscle growth through combined effects. Changes in the concentrations of IGF-I hormone were similar to those of IGF-I gene expression. Our results the mechanism through which MyoD and IGF-I regulate muscle development and demonstrated that MyoD interacted with IGF-I to regulate muscle growth during different developmental stages. Copyright © 2018 Elsevier Inc. All rights reserved.

  20. Genetic differentiation in Japanese flounder in the Yellow Sea and ...

    African Journals Online (AJOL)

    The population structure of Japanese flounder (Paralichthys olivaceus) in the Yellow and East China Seas were analyzed using amplified fragment length polymorphism (AFLP) and cytochrome c oxidase subunit I (COI) gene sequencing. A total of 390 reproducible bands were generated by 10 AFLP primer combinations in ...

  1. An integrated assessment of pollution and biological effects in flounder, mussels and sediment in the southern Baltic Sea coastal area.

    Science.gov (United States)

    Dabrowska, Henryka; Kopko, Orest; Lehtonen, Kari K; Lang, Thomas; Waszak, Ilona; Balode, Maija; Strode, Evita

    2017-02-01

    Organic and metal contaminants and biological effects were investigated in flounder, mussels, and sediments in the southern Baltic Sea coastal area in order to assess environmental quality status in that area. Four sites were selected, including two within the Gulf of Gdańsk (GoG). In biota and sediment at each site, DDTs dominated over PCBs and PBDEs were the least abundant among organic contaminants. Their concentrations decreased progressively outward from GoG. Among metal contaminants, the levels of Hg, Pb, and Cd were elevated in GoG. Biomarkers in flounder, EROD activity and DNA SB, showed moderate positive correlations with organic and metal contaminants. In flounder, the integrated biomarker index (IBR/n) presented a spatial trend coherent with chemical pollution index (CPI), but there was no clear spatial correspondence between IBR/n and CPI in mussels nor between sediment toxicity index (STI) and sediment CPI. The integrated assessment of contaminant and biological effect data against available assessment criteria indicated that in biota, the contaminant assessment thresholds were most often exceeded by CB-118, heptachlor, PBDE, and Hg (in the GoG sediments by p,p'-DDT, Hg and Cd), while of the biological determinants, the threshold was breeched by AChE activity in mussels in GoG. Applying the ICES/OSPAR traffic-light approach showed that of the 50 parameters assessed at each site, there were 18% of determinants in the red color category in the two GoG sites and 8% of determinants in the two sites outside GoG, which indicated that none of the four investigated sites attained good environmental status (GES).

  2. CONSUMPTIONS RATES OF SUMMER FLOUNDER LARVAE ON ROTIFER AND BRINE SHRIMP PREY DURING LARVAL REARING

    Science.gov (United States)

    Larval summer flounder Paralichthys dentatus were hatched and reared through metamorphosis in the laboratory. At several points in the rearing cycle, larvae were removed from their rearing chambers and placed in small bowls, where they were fed known quantities of the rotifer Bra...

  3. Mercury accumulation and the mercury-PCB-sex interaction in summer flounder

    Science.gov (United States)

    Madenjian, Charles P.; Jensen, Olaf P.; Krabbenhoft, David P.; DeWild, John F.; Ogorek, Jacob M.; Vastano, Anthony R.

    2016-01-01

    Patterns in the relative differences in contaminant concentrations between the sexes of mature fish may reveal important behavioral and physiological differences between the sexes. We determined whole-fish total mercury (Hg) concentrations in 23 female summer flounder (Paralichthys dentatus) and 27 male summer flounder from New Jersey coastal waters. To estimate the change in Hg concentration due to release of eggs at spawning, Hg concentration in the somatic tissue and ovaries of 5 of the 23 female summer flounder were also determined. To ascertain whether most of the Hg in the summer flounder was methylmercury (MeHg), whole-fish MeHg concentrations were determined in all 50 summer flounder. Whole-fish Hg concentrations averaged 113 ng/g for females and 111 ng/g for males. Thus, females were 2% higher in Hg concentration than males, on average, but the difference was not statistically significant. Based on Hg determinations in the somatic tissue and ovaries, we predicted that Hg concentration of females would increase by 3.6%, on average, immediately after spawning due to release of eggs. On average, 92% of the Hg in the summer flounder was MeHg. To determine whether the effect of sex on Hg concentration was significantly different from the effect of sex on polychlorinated biphenyl (PCB) concentration, we paired our Hg determinations with PCB determinations from a previous study, and applied regression analysis. Sex significantly interacted with contaminant type (Hg or PCBs), as males were 43% higher in PCB concentration than females, whereas females were 2% higher in Hg concentration than males. Males eliminating Hg from their bodies at a faster rate than females was a likely explanation for this discrepancy between the two contaminant types. Overall, the Hg and PCB concentrations in the summer flounder were relatively low, and therefore our findings also had implications for continued operation of the summer flounder fishery.

  4. Polymorphysims of Gene in the Exons Were Associated with the Reproductive Endocrine of Japanese Flounder (

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    R. Q. Ma

    2012-06-01

    Full Text Available The cytochrome P450c17-I (CYP17-I is one of the enzymes critical to gonadal development and the synthesis of androgens. Two single nucleotide polymorphisms (SNPs were detected within the coding region of the CYP17-I gene in a population of 75 male Japanese flounder (Paralichthys olivaceus. They were SNP1 (c.C445T located in exon2 and SNP2 (c.T980C (p.Phe307Leu located in exon5. Four physiological indices, which were serum testosterone (T, serum 17β-estradiol (E2, Hepatosomatic index (HSI, and Gonadosomatic index (GSI, were studied to examine the effect of the two SNPs on the reproductive endocrines of Japanese flounder. Multiple comparisons revealed that CT genotype of SNP1 had a much lower T level than CC genotype (p<0.05 and the GSI of individuals with CC genotype of SNP2 was higher than those with TT genotype (p<0.05. Four diplotypes were constructed based on the two SNPs and the diplotype D3 had a significantly lower T level and GSI. In conclusion, the two SNPs were significantly associated with reproductive traits of Japanese flounder.

  5. Changes in Hematological, Biochemical and Non-specific Immune Parameters of Olive Flounder, , Following Starvation

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    Jong-Hyun Kim

    2014-09-01

    Full Text Available Triplicate groups of fed and starved olive flounder, Paralichthys olivaceus (body weight: 119.8±17.46 g, were examined over 42 days for physiological changes using hematological, biochemical, and non-specific immune parameters. No significant differences in concentrations of blood hemoglobin and hematocrit and plasma levels of total cholesterol, aspartate aminotransferase, alanine aminotransferase, glucose, and cortisol were detected between fed and starved groups at any sampling time throughout the experiment. In contrast, plasma total protein concentrations were significantly lower in starved fish than in fed fish from day 7 onwards. Moreover, plasma lysozyme concentrations were significantly higher in starved flounder from day 21 onwards. This result confirms that the response of olive flounder to short-term (less than about 1.5 months starvation consists of a readjustment of metabolism rather than the activation of an alarm-stress response. The present results indicate that starvation does not significantly compromise the health status of fish despite food limitation.

  6. Characterizing and predicting the distribution of Baltic Sea flounder (Platichthys flesus) during the spawning season

    Science.gov (United States)

    Orio, Alessandro; Bergström, Ulf; Casini, Michele; Erlandsson, Mårten; Eschbaum, Redik; Hüssy, Karin; Lehmann, Andreas; Ložys, Linas; Ustups, Didzis; Florin, Ann-Britt

    2017-08-01

    Identification of essential fish habitats (EFH), such as spawning habitats, is important for nature conservation, sustainable fisheries management and marine spatial planning. Two sympatric flounder (Platichthys flesus) ecotypes are present in the Baltic Sea, pelagic and demersal spawning flounder, both displaying ecological and physiological adaptations to the low-salinity environment of this young inland sea. In this study we have addressed three main research questions: 1) What environmental conditions characterize the spatial distribution and abundance of adult flounder during the spawning season? 2) What are the main factors defining the habitats of the two flounder ecotypes during the spawning season? 3) Where are the potential spawning areas of flounder? We modelled catch per unit of effort (CPUE) of flounder from gillnet surveys conducted over the southern and central Baltic Sea in the spring of 2014 and 2015 using generalized additive models. A general model included all the stations fished during the survey while two other models, one for the demersal and one for the pelagic spawning flounder, included only the stations where each flounder ecotype should dominate. The general model captured distinct ecotype-specific signals as it identified dual salinity and water depth responses. The model for the demersal spawning flounder revealed a negative relation with the abundance of round goby (Neogobius melanostomus) and a positive relation with Secchi depth and cod abundance. Vegetation and substrate did not play an important role in the choice of habitat for the demersal ecotype. The model for the pelagic spawning flounder showed a negative relation with temperature and bottom current and a positive relation with salinity. Spatial predictions of potential spawning areas of flounder showed a decrease in habitat availability for the pelagic spawning flounder over the last 20 years in the central part of the Baltic Sea, which may explain part of the observed

  7. Steroid sex hormone dynamics during estradiol-17β induced gonadal differentiation in Paralichthys olivaceus (Teleostei)

    Science.gov (United States)

    Sun, Peng; You, Feng; Liu, Mengxia; Wu, Zhihao; Wen, Aiyun; Li, Jun; Xu, Yongli; Zhang, Peijun

    2010-03-01

    Steroid sex hormones, such as estradiol-17β (E2) and testosterone (T), are important regulators of sex change in fish. In this study, we examined the effects of E2 treatment on the dynamics of E2 and T during gonadal differentiation in the olive flounder Paralichthys olivaceus using histology and radioimmunoassay (RIA). Flounder larvae were divided into five groups (G0-G4), and fed with 0 (control), 0.2, 2, 20 and 100 mg E2/kg feed from 35 to 110 day post hatching (dph). Fish growth in the G1 and G2 groups was not significantly different from that of the control group ( P>0.05), while fish in the G3 and G4 groups were less active and showed growth depression and high mortality. The gonads of fish in the G3 and G4 groups were smaller and surrounded by hyperplastic connective tissue. The frequency of females in the G0-G4 groups was 54.5%, 75.0%, 100%, 100% and 93.3%, respectively. The RIA analyses of E2 and T showed that T levels decreased during gonadal differentiation, and increased slightly at the onset of ovarian differentiation, while E2 levels increased gradually and peaked at the onset of ovarian differentiation in the control group. In the E2-treated groups, T levels decreased before the onset of ovarian differentiation. E2 levels were high on the 48 dph, but declined to a lower level on the 54 dph, and then increased gradually during gonadal differentiation. And a sharp increase of E2 levels were observed in all E2-treated groups at the onset of ovarian differentiation. The data suggest that T and E2 play important roles during gonadal differentiation, and an E2 dose of 2 mg/kg feed could induce sex reversal in P. olivaceus.

  8. Optimization of the purification methods for recovery of recombinant growth hormone from Paralichthys olivaceus

    Science.gov (United States)

    Zang, Xiaonan; Zhang, Xuecheng; Mu, Xiaosheng; Liu, Bin

    2013-03-01

    This study aimed to optimize the purification of recombinant growth hormone from Paralichthys olivaceus. Recombinant flounder growth hormone (r-fGH) was expressed by Escherichia coli in form of inclusion body or as soluble protein under different inducing conditions. The inclusion body was renatured using two recovery methods, i.e., dilution and dialysis. Thereafter, the refolded protein was purified by Glutathione Sepharase 4B affinity chromatography and r-fGH was obtained by cleavage of thrombin. For soluble products, r-fGH was directly purified from the lysates by Glutathione Sepharase 4B affinity chromatography. ELISA-receptor assay demonstrated that despite its low receptor binding activity, the r-fGH purified from refolded inclusion body had a higher yield (2.605 mg L-1) than that from soluble protein (1.964 mg L-1). Of the tested recovery methods, addition of renaturing buffer (pH 8.5) into denatured inclusion body yielded the best recovery rate (17.9%). This work provided an optimized purification method for high recovery of r-fGH, thus contributing to the application of r-fGH to aquaculture.

  9. Broodstock management of the fine flounder Paralichthys adspersus (Steindachner, 1867 using recirculating aquaculture systems

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    Lili Carrera

    2013-03-01

    Full Text Available The present study describes the methodology used at IMARPE for the capture, acclimation and management of P. adspersus broodstock using recirculating aquaculture systems (RAS. RAS improved the water quality and maintained the environmental parameters during the acclimation period, temperature (17.2±1°C, oxygen (8.1±0.7 mg L-1, pH (7.3±0.2, ammonia (0.004±0.003 mg L-1, nitrite (0.52±0.2 mg L-1 and nitrate (3.45±2.6 mg L-1. Fish began to be fed normally from day 15 post-capture, once or twice a day using live fish (Odonthestes regia regia, Mugil cephalus, crustacean (Emerita analoga, fresh food (Engraulis ringens and Dosidicus gigas and artificial feed. A significant loss in the weight of the fish was registered during the first days of captivity, followed by a continuous increase in both sexes. The specific growth rate was positive from the third month of captivity, being the relative growth rate 24.5% and 16.2% in August 2010 in males and females, respectively. Different internal and external parasites were detected in the fish, being Entobdella sp. and Philometra sp. the prevailing parasites observed during samplings.

  10. Differences in energy expenditures and growth dilution explain higher PCB concentrations in male summer flounder

    Science.gov (United States)

    Madenjian, Charles P.; Jensen, Olaf P.; Rediske, Richard R.; O'Keefe, James P.; Vastano, Anthony R.; Pothoven, Steven A.

    2016-01-01

    Comparison of polychlorinated biphenyl (PCB) concentrations between the sexes of mature fish may reveal important behavioral and physiological differences between the sexes. We determined whole-fish PCB concentrations in 23 female summer flounder Paralichthys dentatus and 27 male summer flounder from New Jersey coastal waters. To investigate the potential for differences in diet or habitat utilization between the sexes, carbon and nitrogen stable isotope ratios were also determined. In 5 of the 23 female summer flounder, PCB concentrations in the somatic tissue and ovaries were determined. In addition, we used bioenergetics modeling to assess the contribution of the growth dilution effect to the observed difference in PCB concentrations between the sexes. Whole-fish PCB concentrations for females and males averaged 87 and 124 ng/g, respectively; thus males were 43% higher in PCB concentration compared with females. Carbon and nitrogen stable isotope ratios did not significantly differ between the sexes, suggesting that diet composition and habitat utilization did not vary between the sexes. Based on PCB determinations in the somatic tissue and ovaries, we predicted that PCB concentration of females would increase by 0.6%, on average, immediately after spawning due to release of eggs. Thus, the change in PCB concentration due to release of eggs did not explain the higher PCB concentrations observed in males. Bioenergetics modeling results indicated that the growth dilution effect could account for males being 19% higher in PCB concentration compared with females. Thus, the bulk of the observed difference in PCB concentrations between the sexes was not explained by growth dilution. We concluded that a higher rate of energy expenditure in males, stemming from greater activity and a greater resting metabolic rate, was most likely the primary driver for the observed difference in PCB concentrations between the sexes.

  11. Differences in Energy Expenditures and Growth Dilution Explain Higher PCB Concentrations in Male Summer Flounder.

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    Charles P Madenjian

    Full Text Available Comparison of polychlorinated biphenyl (PCB concentrations between the sexes of mature fish may reveal important behavioral and physiological differences between the sexes. We determined whole-fish PCB concentrations in 23 female summer flounder Paralichthys dentatus and 27 male summer flounder from New Jersey coastal waters. To investigate the potential for differences in diet or habitat utilization between the sexes, carbon and nitrogen stable isotope ratios were also determined. In 5 of the 23 female summer flounder, PCB concentrations in the somatic tissue and ovaries were determined. In addition, we used bioenergetics modeling to assess the contribution of the growth dilution effect to the observed difference in PCB concentrations between the sexes. Whole-fish PCB concentrations for females and males averaged 87 and 124 ng/g, respectively; thus males were 43% higher in PCB concentration compared with females. Carbon and nitrogen stable isotope ratios did not significantly differ between the sexes, suggesting that diet composition and habitat utilization did not vary between the sexes. Based on PCB determinations in the somatic tissue and ovaries, we predicted that PCB concentration of females would increase by 0.6%, on average, immediately after spawning due to release of eggs. Thus, the change in PCB concentration due to release of eggs did not explain the higher PCB concentrations observed in males. Bioenergetics modeling results indicated that the growth dilution effect could account for males being 19% higher in PCB concentration compared with females. Thus, the bulk of the observed difference in PCB concentrations between the sexes was not explained by growth dilution. We concluded that a higher rate of energy expenditure in males, stemming from greater activity and a greater resting metabolic rate, was most likely the primary driver for the observed difference in PCB concentrations between the sexes.

  12. Characterization and functional analysis of the Paralichthys olivaceus prdm1 gene promoter.

    Science.gov (United States)

    Li, Peizhen; Wang, Bo; Cao, Dandan; Liu, Yuezhong; Zhang, Quanqi; Wang, Xubo

    2017-10-01

    PR domain containing protein 1 (Prdm1) is a transcriptional repressor identified in various species and plays multiple important roles in immune response and embryonic development. However, little is known about the transcriptional regulation of the prdm1 gene. This study aims to characterize the promoter of Paralichthys olivaceus prdm1 (Po-prdm1) gene and determine the regulatory mechanism of Po-prdm1 expression. A 2000bp-long 5'-flanking region (translation initiation site designated as +1) of the Po-prdm1 gene was isolated and characterized. The regulatory elements in this fragment were then investigated and many putative transcription factor (TF) binding sites involved in immunity and multiple tissue development were identified. A 5'-deletion analysis was then conducted, and the ability of the deletion mutants to promote luciferase and green fluorescent protein (GFP) expression in a flounder gill cell line was examined. The results revealed that the minimal promoter is located in the region between -446 and -13bp, and the region between -1415 and -13bp enhanced the promoter activity. Site-directed mutation analysis was subsequently performed on the putative regulatory elements sites, and the results indicated that FOXP1, MSX and BCL6 binding sites play negative functional roles in the regulation of the Po-prdm1 expression in FG cells. In vivo analysis demonstrated that a GFP reporter gene containing 1.4kb-long promoter fragment (-1415/-13) was expressed in the head and trunk muscle fibres of transient transgenic zebrafish embryos. Our study provided the basic information for the exploration of Po-prdm1 regulation and expression. Copyright © 2017 Elsevier Inc. All rights reserved.

  13. Gene expression patterns regulating embryogenesis based on the integrated de novo transcriptome assembly of the Japanese flounder.

    Science.gov (United States)

    Fu, Yuanshuai; Jia, Liang; Shi, Zhiyi; Zhang, Junling; Li, Wenjuan

    2017-06-01

    The Japanese flounder (Paralichthys olivaceus) is one of the most important commercial and biological marine fishes. However, the molecular biology involved during embryogenesis and early development of the Japanese flounder remains largely unknown due to a lack of genomic resources. A comprehensive and integrated transcriptome is necessary to study the molecular mechanisms of early development and to allow for the detailed characterization of gene expression patterns during embryogenesis; this approach is critical to understanding the processes that occur prior to mesectoderm formation during early embryonic development. In this study, more than 117.8 million 100bp PE reads were generated from pooled RNA extracted from unfertilized eggs to 41dph (days post-hatching) embryos and were sequenced using Illumina pair-end sequencing technology. In total, 121,513 transcripts (≥200bp) were obtained using de novo assembly. A sequence similarity search indicated that 52,338 transcripts show significant similarity to 22,462 known proteins from the NCBI non-redundant database and the Swiss-Prot protein database and were annotated using Blast2GO. GO terms were assigned to 44,627 transcripts with 12,006 functional terms, and 10,024 transcripts were assigned to 133 KEGG pathways. Furthermore, gene expression differences between the unfertilized egg and the gastrula embryo were analysed using Illumina RNA-Seq with single-read sequencing technology, and 24,837 differentially and specifically expressed transcripts were identified and included 5,286 annotated transcripts and 19,569 non-annotated transcripts. All of the expressed transcripts in the unfertilized egg and gastrula embryo were further classified as maternal, zygotic, or maternal-zygotic transcripts, which may help us to understand the roles of these transcripts during the embryonic development of the Japanese flounder. Thus, the results will contribute to an improved understanding of the gene expression patterns and

  14. Effects of Dietary Extract on Growth, Feed Utilization and Challenge Test of Olive Flounder (

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    S. H. Cho

    2013-01-01

    Full Text Available Effects of dietary Scutellaria baicalensis extract (SBE on growth, feed utilization and challenge test of olive flounder (Paralichthys olivaceus were determined. Six hundred thirty fish averaging 5.0 g were distributed into 18, 180-L tanks. Six experimental diets were prepared in triplicate: SBE-0, SBE-0.5, SBE-1, SBE-2, SBE-3 and SBE-5 diets containing SBE at the concentrations of 0, 0.5, 1, 2, 3 and 5%, respectively. Fish were hand-fed to apparent satiation twice a day for 8 wks. At the end of 8-wk feeding trial, ten fish from each tank were infected by Edwardsiella tarda for challenge test. Survival and weight gain of fish were not affected by dietary concentrations of SBE. However, specific growth rate of fish fed the SBE-2 diet was higher than that of fish fed the SBE-0, SBE-1, SBE-3 and SBE-5 diets. Neither feed efficiency nor serum chemical composition of fish was affected by dietary concentrations of SBE. The cumulative mortality of fish fed the SBE-0 diet was 100% at 96 h after E. tarda infection, but 77 to 87% for fish fed the other diets. Dietary inclusion of 2% SBE appears to be recommendable to improve specific growth rate of fish and SBE had the potential to mitigate mortality of fish at E. tarda infection.

  15. Functional relevance of three proopiomelanocortin (POMC) genes in darkening camouflage, blind-side hypermelanosis, and appetite of Paralichthys olivaceus.

    Science.gov (United States)

    Kang, Duk-Young; Kim, Hyo-Chan

    2015-01-01

    To determine whether proopiomelanocortin (POMC) genes are involved in darkening color camouflage, blind-side hypermelanosis, and appetite in flatfish, we isolated and cloned three POMC genes from the pituitary of the olive flounder (Paralichthys olivaceus) and compared their amino acid (aa) structures to those of POMC genes from other animals. Next, we examined the relationship of these pituitary POMC genes to camouflage color change, blind-side hypermelanosis, and appetite by quantifying mRNA expression. Olive flounder (of)-POMC1, 2, and 3 cDNAs consisted of 648-bp, 582-bp, and 693-bp open reading frames (ORF) encoding 216 aa, 194 aa, and 231 aa residues, respectively. Structurally, the three of-POMC cDNAs consisted of seven peptides (signal peptide, N-POMC, α-MSH, CLIP, N-β-LPH, β-MSH and β-END [or END-like peptide]) that are similar to those of other fish POMC cDNAs. α-MSH encoded a protein composed of 13 aa and β-MSH encoded a protein composed of 17 aa. The three POMC genes were predominantly expressed in the pituitary gland, but they were also expressed in a variety of tissues, including brain, eye, kidney, heart, testis, and skin. of-POMC2 exhibited the highest expression, while of-POMC3 displayed the lowest expression. The relative levels of of-POMC1 and 3 mRNAs were not influenced by background color and feeding (or fasting), but the relative level of of-POMC2 mRNA significantly increased in response to a dark background and fasting. The relative levels of of-POMC1 and 2 mRNAs were significantly higher in hypermelanic fish; however, we did not determine a direct anorexigenic or orexigenic relationship for the three POMC genes. These results indicate that pituitary POMC genes are related to darkening color change and the differentiation of pigment cells, but they are not directly related to appetite. Copyright © 2014 Elsevier Inc. All rights reserved.

  16. 50 CFR Figure 10 to Part 223 - Flounder TED

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Flounder TED 10 Figure 10 to Part 223 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS THREATENED MARINE AND ANADROMOUS SPECIES Pt. 223, Fig. 10 Figure 10 to Part 223—Flounder TED EC01JY91.056 [5...

  17. Brasilochondria riograndensis gen. nov., sp. nov. (Copepoda, Chondracanthidae a parasite of flounders of Rio Grande do Sul State, Brazil

    Directory of Open Access Journals (Sweden)

    Vernon E. Thatcher

    2004-09-01

    Full Text Available Brasilochondria riograndensis gen. nov., sp. nov. (Copepoda, Chondracanthidae a parasite of the flounder, Paralichthys orbignyanus (Valenciennes, 1839, is described. The new genus has a spherical head a post-mandibular "neck" and two pairs of modified biramous legs. In these respects, it resembles Argentinochondria patagonensis Etchegoin, Timi & Sardella, 2003. In the Argentine genus, however, the bulbous head has a medial constriction and the posterior of the female lacks the lateral extensions that are present in the new genus. Pseudolernentoma brasiliensis Luque & Alves, 2003, also resembles the new genus but it lacks the lateral extensions of the trunk and the latter is cylindrical rather than flat. The second leg of the new genus is small and the endopod is shorter than the exopod. The other two genera have large second legs with subequal rami.Brasilochondria riograndensis gen. nov., sp. nov. (Copepoda, Chondracanthidae, um parasito do linguado, Paralichthys orbignyanus (Valenciennes, 1839, é descrito. O novo gênero tem uma cabeça esférica, um "pescoço" pós-mandíbular e dois pares de pernas que são birremes e modificados na fêmea. Nestes aspectos, ela parece com Argentinochondria patagonensis Etchegoin, Timi & Sardella, 2003. Nesta, no entanto, a cabeça é esférica com uma constrição medial e na parte posterior do tronco faltam as extensões póstero-laterais que o novo gênero possui. Pseudolernentoma brasiliensis Luque & Alves, 2003, é também parecido com o novo gênero, mas carece das extensões póstero-laterais e o mesmo tronco é cilíndrico em vez de achatado. A segunda perna no novo gênero é pequena e o endopodito é mais curto que o exopodito. Nos outros dois gêneros, as segundas pernas são grandes e os ramos são sub-iguais.

  18. Adaptive differences in gene expression in European flounder ( Platichthys flesus )

    DEFF Research Database (Denmark)

    Larsen, Peter Foged; Eg Nielsen, Einar; Williams, T.D.

    2007-01-01

    levels of neutral genetic divergence, a high number of genes were significantly differentially expressed between North Sea and Baltic Sea flounders maintained in a long-term reciprocal transplantation experiment mimicking natural salinities. Several of the differentially regulated genes could be directly...... linked to fitness traits. These findings demonstrate that flounders, despite little neutral genetic divergence between populations, are differently adapted to local environmental conditions and imply that adaptation in gene expression could be common in other marine organisms with similar low levels...

  19. The influence of cage conditioning on the performance and behavior of Japanese flounder reared for stock enhancement: Burying, feeding, and threat response

    Science.gov (United States)

    Walsh, Michelle L.; Masuda, Reiji; Yamashita, Yoh

    2014-01-01

    Flatfish reared for stock enhancement often exhibit irregular behavioral patterns compared with wild conspecifics. These “deficits”, mostly attributed to the unnatural characteristics of the hatchery environment, are assumed to translate to increased predation risk. Initially releasing fish in predator-free conditioning cages may help flatfish adjust to the wild environment, establish burial skills, begin pigment change, recover from transport stress, and experience natural (live) food sources before full release into the wild. However, the impact of cage conditioning on the performance and behavior of flatfish has yet to be fully assessed. We conducted video trials with 10-cm, hatchery-reared Japanese flounder, Paralichthys olivaceus, in sand-bottomed aquaria to assess four treatments of flounder: (1) reared fish cage conditioned for 7 d in the shallow coast, (2) reared fish directly from hatchery tanks, (3) wild fish, and (4) reared fish released directly from hatchery tanks into the wild and then recaptured after 6 d at large. Burying ability, predation, and threat response to a model predator were examined. Wild fish buried most, followed by cage conditioned, and released-then-recaptured and non-conditioned (directly from tank) fish. Wild and conditioned fish revealed much lower variation in total movement duration, which corresponded with lower levels and variation in prey vertical movement. Fish of all condition types exhibited a lower number of attacks and off-bottom swimming events, and a lower movement duration when the model predator was in motion versus when it was still. This study is the first to evaluate the behavioral mechanisms of hatchery-reared flatfish that have been cage-conditioned or released-then-recaptured. In addition, we provide evidence that cage conditioning can enhance the performance of released flatfish.

  20. Use of a Marine Vertebrate, the Flounder, in the Physiology Teaching Laboratory

    Science.gov (United States)

    Bruce, David S.; Linden, Donald G.

    1973-01-01

    Describes two experiments in physiology using a readily available marine vertebrate, the flounder. Representative results are presented from experiments which measure the effect of excess potassium on the flounder EKG, and the effect of temperature on heart rate of the starry flounder. (JR)

  1. Myostatin inhibitory region of fish (Paralichthys olivaceus) myostatin-1 propeptide.

    Science.gov (United States)

    Lee, Sang Beum; Kim, Jeong Hwan; Jin, Deuk-Hee; Jin, Hyung-Joo; Kim, Yong Soo

    2016-01-01

    Myostatin (MSTN) is a potent negative regulator of skeletal muscle growth, and its activity is suppressed by MSTN propeptide (MSTNpro), the N-terminal part of MSTN precursor cleaved during post-translational MSTN processing. The current study examined which region of flatfish (Paralichthys olivaceus) MSTN-1 propeptide (MSTN1pro) is critical for MSTN inhibition. Six different truncated forms of MSTN1pro containing N-terminal maltose binding protein (MBP) as a fusion partner were expressed in Escherichia coli, and partially purified by an affinity chromatography for MSTN-inhibitory activity examination. Peptides covering different regions of flatfish MSTN1pro were also synthesized for MSTN-inhibitory activity examination. A MBP-fused MSTN1pro region consisting of residues 45-100 had the same MSTN-inhibitory potency as the full sequence flatfish MSTN1pro (residues 23-265), indicating that the region of flatfish MSTN1pro consisting of residues 45-100 is sufficient to maintain the full MSTN-inhibitory capacity. A MBP-fused MSTN1pro region consisting of residues 45-80 (Pro45-80) also showed MSTN-inhibitory activity with a lower potency, and the Pro45-80 demonstrated its MSTN binding capacity in a pull-down assay, indicating that the MSTN-inhibitory capacity of Pro45-80 is due to its binding to MSTN. Flatfish MSTN1pro synthetic peptides covering residues 45-65, 45-70, and 45-80 demonstrated MSTN-inhibitory activities, but not the synthetic peptide covering residues 45-54, indicating that residues 45-65 of flatfish MSTN1pro are essential for MSTN inhibition. In conclusion, current study show that like the mammalian MSTNpro, the MSTN-inhibitory region of flatfish MSTN1pro resides near its N-terminus, and imply that smaller sizes of MSTNpro can be effectively used in various applications designed for MSTN inhibition. Copyright © 2016 Elsevier Inc. All rights reserved.

  2. Taxonomy of the early life stages of arrowtooth flounder (Atheresthes stomias) and Kamchatka flounder (A. evermanni) in the eastern Bering Sea, with notes on distribution and condition

    Science.gov (United States)

    De Forest, Lisa; Duffy-Anderson, J. T.; Heintz, R. A.; Matarese, A. C.; Siddon, E. C.; Smart, T. I.; Spies, I. B.

    2014-11-01

    Arrowtooth flounder (Atheresthes stomias) and Kamchatka flounder (A. evermanni) are closely related flatfish species that co-occur in the eastern Bering Sea. As adults, arrowtooth flounder can be distinguished from Kamchatka flounder; however, larvae and early juveniles can only be indentified to the genus level due to morphological similarities. This has precluded studies of ecology for the early life stages of both species in the eastern Bering Sea. In this study, we developed a genetic technique to identify the larvae and early juveniles of the two species using mtDNA cytochrome oxidase subunit I (COI). Genetically identified specimens were then examined to determine a visual identification method based on pigment patterns and morphology. Specimens 6.0-12.0 mm SL and≥18.0 mm SL can be identified to the species level, but species identification of individuals 12.1-17.9 mm SL by visual means alone remains elusive. The distribution of larvae (<25.0 mm SL) of both arrowtooth flounder and Kamchatka flounder is similar in the eastern Bering Sea; however, juvenile (≥25.0 mm SL) Kamchatka flounder occur closer to the shelf break and in deeper water than juvenile arrowtooth flounder. Condition was determined for larvae and juveniles of each species by analyzing lipid content (%) and energy density (kJ/g dry mass). Kamchatka flounder larvae on average had higher lipid content than arrowtooth flounder larvae, but were also larger on average than arrowtooth flounder larvae in the summer. When corrected for length, both species had similar lipid content in the larval and juvenile stages.

  3. Juvenile nursery colonization patterns for the European flounder (

    NARCIS (Netherlands)

    Martinho, F.; van der Veer, H.W.; Cabral, H.N.; Pardal, M.A.

    2013-01-01

    In this work, we analysed the latitudinal trends in the nursery habitat colonization processes of the European flounder (Platichthys flesus). This was accomplished by estimating the duration of the pelagic and metamorphic stages, as well as the duration of the spawning period, in several nursery

  4. Nutritional status modulates plasma leptin, AMPK and TOR activation, and mitochondrial biogenesis: Implications for cell metabolism and growth in skeletal muscle of the fine flounder.

    Science.gov (United States)

    Fuentes, Eduardo N; Safian, Diego; Einarsdottir, Ingibjörg Eir; Valdés, Juan Antonio; Elorza, Alvaro A; Molina, Alfredo; Björnsson, Björn Thrandur

    2013-06-01

    Insight of how growth and metabolism in skeletal muscle are related is still lacking in early vertebrates. In this context, molecules involved in these processes, such as leptin, AMP-activated protein kinase (AMPK), target of rapamicyn (TOR), peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α, and oxidative phosphorylation complexes (OXPHOS), were assessed in the skeletal muscle of a fish species. Periods of fasting followed by a period of refeeding were implemented, using the fine flounder as a model (Paralichthys adspersus). This species exhibits remarkably slow growth and food intake, which is linked to an inherent growth hormone (GH) resistance and high circulating levels of leptin. Leptin increased during fasting concomitantly with AMPK activation, which was inversely correlated with TOR activation. On the other hand, AMPK was directly correlated with an increase in PGC-1α and OXPHOS complexes contents. Dramatic changes in the activation and content of these molecules were observed during short-term refeeding. Leptin, AMPK activation, and PGC-1α/OXPHOS complexes contents decreased radically; whereas, TOR activation increased significantly. During long-term refeeding these molecules returned to basal levels. These results suggest that there is a relation among these components; thus, during fasting periods ATP-consuming biosynthetic pathways are repressed and alternative sources of ATP/energy are promoted, a phenomenon that is reversed during anabolic periods. These results provide novel insight on the control of metabolism and growth in the skeletal muscle of a non-mammalian species, suggesting that both processes in fish muscle are closely related and coordinated by a subset of common molecules. Copyright © 2013 Elsevier Inc. All rights reserved.

  5. 77 FR 76942 - 2013-2014 Summer Flounder and Scup Specifications; 2013 Black Sea Bass Specifications...

    Science.gov (United States)

    2012-12-31

    ... Regulatory Flexibility Analysis (IRFA), and other supporting documents used by the Summer Flounder, Scup, and... http://www.nero.noaa.gov . The Final Regulatory Flexibility Analysis (FRFA) consists of the IRFA... Specifications Summer Flounder Scup Black Sea Bass 2013 2014 2013 2014 2013 ABC million lb 22.34 22.24 38.71 35...

  6. Paralichthys patagonicus spawning areas and reproductive potential in the Bonaerense Coastal Zone, Argentina (34°-42°S Áreas de desove y potencial reproductivo de Paralichthys patagonicus en la Zona Costera Bonaerense, Argentina (34°-42°S

    Directory of Open Access Journals (Sweden)

    María Inés Militelli

    2011-01-01

    Full Text Available The reproductive biology of Patagonian flounder (Paralichthys patagonicus was studied in the Bonaerense Coastal Zone (34°-42°S using samples collected in December 2003 and 2005. According to the literature, the data were analyzed considering two population groups: one in the northern zone (34°-38°S and another in El Rincón (38°-42°S. The estimated size at first maturity for both sexes in 2003 was 31.57 cm TL for the northern zone and 29.58 cm TL for El Rincón. In 2005, the L50 was only estimated for the northern zone, and the value obtained was less than that of 2003 (27.82 cm TL. In December 2003, the greatest reproductive activity took place in El Rincón. However, in December 2005, the highest proportions of hydrated fe-males were observed in the northern area. This could be due to the cooler temperatures recorded for this zone, as compared with 2003. Batch fecundity showed a potential fit to size and a lineal fit to weight and ranged from 14,685 (30 cm TL to 153,717 ( 59 cm TL hydrated oocytes. Relative fecundity oscillated between 24 and 170 hydrated oocytes/g. The values of these parameters differed between years and among population groups. These differences could be attributed in part to the difference in seawater temperature between the two years, especially the high temperatures observed in 2005.Se estudió la biología reproductiva del lenguado (Paralichthys patagonicus en la Zona Costera Bonaerense (34°-42°S. El material provino de muestreos realizados durante diciembre de 2003 y 2005. En base a la bibliografía los datos fueron analizados teniendo en cuenta dos grupos poblacionales, uno en el norte (34-38°S y otro en El Rincón (38-42°S. La estimación de la talla de primera madurez en 2003 para ambos sexos fue de 31.57 cm. LT para la zona norte y de 29.58 cm LT para El Rincón. En 2005 solo se pudo estimar el L50 para la zona norte y el valor obtenido fue menor al 2003 (27.82 cm. LT. En diciembre del 2003 la mayor

  7. Simulating the impact of the entrainment of winter flounder larvae

    International Nuclear Information System (INIS)

    Hess, K.W.; Sissenwine, M.P.; Saila, S.B.

    1975-01-01

    The transport of winter flounder larvae around the Millstone Point, Conn. Area by the action of tidal currents and diffusion was simulated by computer to predict the numbers which could be entrained during the operation of a local nuclear power station. A tidal hydrodynamic model with variable depth was employed to simulate currents and water levels. These techniques provided input to a transport model which simulated the concentration of larvae. A larval source in a tributary river was simulated for twenty tidal cycles, with and without entrainment. The results indicated that the reduction in winter flounder larvae near Millstone Point at the end of the pelagic stage (period during which larvae are likely to be entrained) was less than 1 percent when it was assumed that larvae have little chance of returning once lost from Millstone bight. In order to assess the effect of a 1 percent reduction in recruitment of winter flounder larvae to the benthic phase of their life cycle, the local population was simulated by a model in which year-classes and the total egg production were represented by compartments. Each year-class grew, produced eggs, suffered natural and fishing mortality according to information derived from the literature. The effect of power plant entrainment was incorporated by reducing the number of recruits to year-class I that would normally result from a specific level of egg production. For a 1 percent reduction in recruitment due to power plant entrainment, a potential 6 percent decrease in total population size following 35 years of operation was indicated. (U.S.)

  8. Early growth and development of reciprocal hybrids of the starry flounder Platichthys stellatus and stone flounder Kareius bicoloratus.

    Science.gov (United States)

    Yamashita, Y T; Aritaki, M; Kurita, Y; Tanaka, M

    2014-05-01

    Larval growth and development of hybrid flounder were observed and compared with those of their parent species. The reciprocal hybrids of female starry flounder Platichthys stellatus and male stone flounder Kareius bicoloratus (hybrid Sb) and of female K. bicoloratus and male P. stellatus (hybrid Bs) both survived and grew to juveniles. Development was divided into nine stages (A-I). Many of the hybrids' traits were identical and intermediate to those of their parents. The position of the eye, however, was primarily sinistral in both hybrids (80% in Sb and 76% in Bs), a trait possessed by P. stellatus (80%) in the western Pacific Ocean. The daily growth rates of the larvae were similar. In the parent species, development was more rapid in P. stellatus than in K. bicoloratus whereas rate of development was intermediate in both Sb and Bs hybrids. The size at settlement [standard length (LS ) at stage H (mean ± s.d.)] was 9·82 ± 1·47 mm for the hybrid Sb and 9·99 ± 0·90 mm for the hybrid Bs, while the minimum age at metamorphosis (initial age at stage H) was 29 days after hatching (DAH) in both hybrids. In comparison, LS at settlement in parent species was 6·43 ± 0·25 mm for P. stellatus and 12·87 ± 1·29 mm for K. bicoloratus. Minimum age at metamorphosis for the parents was 23 DAH at stage G in P. stellatus and 34 DAH at stage H in K. bicoloratus. Thus, the timing of settlement of hybrids was different from that of their parent species. These traits may occur with high frequency in a natural habitat. © 2014 The Fisheries Society of the British Isles.

  9. Changeable camouflage: how well can flounder resemble the colour and spatial scale of substrates in their natural habitats?

    Science.gov (United States)

    Akkaynak, Derya; Siemann, Liese A; Barbosa, Alexandra; Mäthger, Lydia M

    2017-03-01

    Flounder change colour and pattern for camouflage. We used a spectrometer to measure reflectance spectra and a digital camera to capture body patterns of two flounder species camouflaged on four natural backgrounds of different spatial scale (sand, small gravel, large gravel and rocks). We quantified the degree of spectral match between flounder and background relative to the situation of perfect camouflage in which flounder and background were assumed to have identical spectral distribution. Computations were carried out for three biologically relevant observers: monochromatic squid, dichromatic crab and trichromatic guitarfish. Our computations present a new approach to analysing datasets with multiple spectra that have large variance. Furthermore, to investigate the spatial match between flounder and background, images of flounder patterns were analysed using a custom program originally developed to study cuttlefish camouflage. Our results show that all flounder and background spectra fall within the same colour gamut and that, in terms of different observer visual systems, flounder matched most substrates in luminance and colour contrast. Flounder matched the spatial scales of all substrates except for rocks. We discuss findings in terms of flounder biology; furthermore, we discuss our methodology in light of hyperspectral technologies that combine high-resolution spectral and spatial imaging.

  10. Parasites of the flatfish Paralichthys adspersus (Steindachner, 1867 (Pleuronectiformes from northern Chile

    Directory of Open Access Journals (Sweden)

    Marcelo E Oliva

    1996-06-01

    Full Text Available Three species of protistan and 22 species of metazoan parasites were obtained from a sample of 179 flatfish, (Paralichthys adspersus taken-off Antofagasta, northern Chile. Prevalence of infection of seven parasites (Protista: 1, Copepoda: 2, Digenea: 1, Acantocephala: 1, Nematoda: 2 was significantly and positively correlated with host size. Host's sex do not seem to affect prevalence of infection, except for Nybelinia surmenicola, Capillaria sp. and Anisakis sp. (prevalence of infection significantly greater in males than females and Philometra sp. (prevalence higher in females. Mean abundance is correlated with size in nine species (Protista: 1, Copepoda: 2, Digenea: 3, Acantocephala: 1, Nematoda: 2. Host's sex do not affect mean abundance, except for Cainocreadium sp. and Philometra sp.(mean abundance higher in females and Nybelinia surmenicola, Capillaria sp. and Anisakis sp. (mean abundance higher in males.

  11. The influence of environmental conditions on early life stages of flounder (Platichthys flesus) in the central Baltic Sea

    Science.gov (United States)

    Ustups, Didzis; Müller-Karulis, Bärbel; Bergstrom, Ulf; Makarchouk, Andrej; Sics, Ivo

    2013-01-01

    Flounder (Platichthys flesus) is a temperate marine fish that is well adapted to the brackish waters of the Baltic Sea. There are two sympatric flounder populations in the Baltic Sea, pelagic and demersal spawners, which differ in their spawning habitat and egg characteristics. In the present study, pelagic spawning flounder of the central Baltic Sea was studied. We examined whether variations in hydrological regime can explain fluctuations in flounder early life stages that have occurred over the past 30 years (1970-2005). Using generalized additive modeling to explain the abundance of flounder eggs and larvae in a Latvian ichthyoplankton dataset, we evaluate the hypothesis that the available reproductive volume, defined as the water column with dissolved oxygen larger than 1 ml/l and salinity between 10.6 and 12 PSU, affects the survival of flounder ichthyoplankton and determines recruitment success. Both reproductive volume and spawning stock biomass were significant factors determining flounder ichthyoplankton abundance. Different measures of water temperature did not contribute significantly to the variability of eggs or larvae. However, recruitment did not correlate to the supply of larvae. The findings presented in this study on the relationship between flounder reproduction, spawning stock biomass and reproductive volume, as well as the lack of correlation to recruitment, are valuable for the understanding of flounder ecology in the Baltic Sea, and for developing the management of the species.

  12. Immigration and early life stages recruitment of the European flounder (Platichthys flesus) to an estuarine nursery: The influence of environmental factors

    Science.gov (United States)

    Amorim, Eva; Ramos, Sandra; Elliott, Michael; Bordalo, Adriano A.

    2016-01-01

    Connectivity between coastal spawning grounds and estuarine nurseries is a critical step in the life cycle of many fish species. Larval immigration and transport-associated physical-biological processes are determinants of recruitment success to nursery areas. The recruitment of the European flounder, Platichthys flesus, to estuarine nurseries located at the southern edge of the species distribution range, has been usually investigated during its juvenile stages, while estuarine recruitment during the earlier planktonic life stage remains largely unstudied. The present study investigated the patterns of flounder larval recruitment and the influence of environmental factors on the immigration of the early life stages to the Lima estuary (NW Portugal), integrating data on fish larvae and post-settlement individuals (< 50 mm length), collected over 7 years. Late-stage larvae arrived at the estuary between February and July and peak abundances were observed in April. Post-settlement individuals (< 50 mm) occurred later between April and October, whereas newly-settled ones (< 20 mm) were found only in May and June. Variables associated with the spawning, survival and growth of larvae in the ocean (sea surface temperature, chlorophyll a and inland hydrological variables) were the major drivers of flounder occurrence in the estuarine nursery. Although the adjacent coastal area is characterized by a current system with strong seasonality and mesoscale variability, we did not identify any influence of variables related with physical processes (currents and upwelling) on the occurrence of early life stages in the estuary. A wider knowledge on the influence of the coastal circulation variability and its associated effects upon ocean-estuarine connectivity is required to improve our understanding of the population dynamics of marine spawning fish that use estuarine nurseries.

  13. Effects of Dietary Lipid Source and Level on Growth Performance, Blood Parameters and Flesh Quality of Sub-adult Olive Flounder (

    Directory of Open Access Journals (Sweden)

    Dong-Kyu Kim

    2012-06-01

    Full Text Available This study was conducted to investigate the effects of dietary lipid source and level on growth performance, blood parameters, fatty acid composition and flesh quality of sub-adult olive flounder Paralichthys olivaceus. Eight experimental diets were formulated to contain 5% squid liver oil (SLO, 5% linseed oil (LO, 5% soybean oil (SO, a mixture of 1% squid liver oil, 2% linseed oil and 2% soybean oil (MIX, no lipid supplementation with high protein level (LL-HP, 10% squid liver oil (HL-SLO, a mixture of 1% squid liver oil, 4.5% linseed oil and 4.5% soybean oil (HL-VO, and 1% squid liver oil with high starch level (LL-HC, respectively. Two replicate groups of fish (average initial weight of 296 g were fed the diets for 17 wks. After 5 wks, 11 wks and the end of the feeding trial, five fish from each tank were randomly sampled for analysis of body composition. At the end of the feeding trial, final mean weight of fish fed the LL-HP diet was significantly (p<0.05 higher than that of fish fed the HL-VO diet, but did not differ significantly from those of fish fed the SLO, LO, SO, MIX, HL-SLO and LL-HC diets. Fish fed the LL-HP diet showed significantly higher feed efficiency than fish fed the LO, HL-SLO and HL-VO diets. Feed efficiency of fish fed the LO, SO and MIX diets were similar to those of fish fed the SLO and HL-SLO diets. Fish fed the HL-SLO diet showed significantly higher total cholesterol content in plasma compared with other diets. Fatty acid composition of tissues was reflected by dietary fatty acid composition. The highest linoleic (LA and linolenic acid (LNA contents in the dorsal muscle were observed in fish fed the SO and LO diets, respectively, regardless of feeding period. The highest eicosapentaenoic acid (EPA content in the dorsal muscle was observed in fish fed the LL-HP and LL-HC diets after 11 and 17 weeks of feeding, respectively. Fish fed the SLO and HL-SLO diets showed higher docosahexaenoic acid (DHA content than that of

  14. Liver hypertrophy in winter flounder following exposure to experimentally oiled sediments

    Energy Technology Data Exchange (ETDEWEB)

    Fletcher, G L; King, M J; Kiceniuk, J W; Addison, R F

    1982-01-01

    1. Male winter flounder were exposed to sediments contaminated with Venezuelan crude oil in 3 laboratory experiments of 4-5 months duration. 2. Oil exposure resulted in significant increases in liver weight. This was particularly evident in fish weighing less than 400 g. 3. The enlarged livers of the oil-exposed flounder had reduced concentrations of DNA, protein, Na/sup +/ and Zn2/sup +/, and increased concentrations of lipid and phospholipid. 4. The reduced DNA and Na/sup +/ concentrations suggested liver hypertrophy rather than hyperplasia. 5. The increased phospholipid concentrations suggested growth of membrane structures such as endoplasmic reticulum.

  15. Characterizing and predicting the distribution of Baltic Sea flounder (Platichthys flesus) during the spawning season

    DEFF Research Database (Denmark)

    Orio, Alessandro; Bergström, Ulf; Casini, Michele

    2017-01-01

    , both displaying ecological and physiological adaptations to the low-salinity environment of this young inland sea. In this study we have addressed three main research questions: 1) What environmental conditions characterize the spatial distribution and abundance of adult flounder during the spawning...... a decrease in habitat availability for the pelagic spawning flounder over the last 20 years in the central part of the Baltic Sea, which may explain part of the observed changes in populations' biomass. We conclude that spatiotemporal modelling of habitat availability can improve our understanding of fish...

  16. High doses of corticosteroid suppress resistance to Ichthyophonus in starry flounder

    Science.gov (United States)

    Perry, J.A.; Kocan, R.M.; Winton, J.R.; Hershberger, P.K.

    2004-01-01

    Application of pharmacological doses of the corticosteroid dexamethasone phosphate to starry flounder Platichthys stellatus resulted in a predisposition to clinical ichthyophoniasis and a progression from latent Ichthyophonus infections to patent, histologically identifiable infections. Among Ichthyophonus-challenged starry flounder, the prevalences of clinical infections and histologically identifiable infections were significantly greater in two groups that received dexamethasone (100% and 31%, respectively) than in the respective control groups (8% and 0%). Proliferation of Ichthyophonus infections in corticosteroid-treated groups may have resulted from suppression of the cellular immune response that typically follows corticosteroid application; however, further studies are needed to determine whether these effects occur at lower, physiological concentrations of corticosteroids.

  17. Is the World Floundering or Has She a Vision?

    Directory of Open Access Journals (Sweden)

    Ashok Natarajan

    2015-05-01

    Full Text Available To all appearances the world seems to be floundering without leadership, direction or even a clear formulation of goals and processes. Yet, paradoxically, amidst the apparent chaos and confusion, evolutionary patterns of advance somehow seem to emerge, universal values become ever more prevalent and powerful as deep drivers and determinates, a more than conventional wisdom seems to guide situations where conventional wisdom is stymied or blinded by dogma and superstition, and susceptible ignorant masses sooner or later choose a course that leads to the future rather than back to the past. This progressive movement is far from steady and often interspersed with retrograde steps and descends into utter folly and vicious violence, but all the same a direction seems to emerge that defies the expectations of scientific projections and doomsday prophets. Despite our adoration of rationality, irrationality prevails even in the halls of knowledge. Bursts of extraordinary creativity follow episodes of suicidal stupidity. Humanity’s predilection to self-destruction exists side by side with a serendipitous capacity for creativity and coming to our senses just in the nick of time to avert calamity. Pessimists decry the negative tendency. Optimists affirm the positive. None seem able to discern the pattern or process by which challenges become opportunities and imminent disaster is transmuted into progress. A key to deciphering this enigma lies in the invisible, yet to be realized potentials of the individual and society. This clumsy, costly, painful process is the mark of a still largely unconscious species struggling to discover its raison d’être and the secret of conscious evolution. Utopian ideals discredited by a world disillusioned by false promises exhibit a remarkable regenerative power to advance toward unseen goals. An unrealized vision founded on universal values guides us toward an inevitable destiny we have yet to conceive. Those seed

  18. Geographical variation in the age/length relationship in Baltic flounder ( Platichthys flesus )

    DEFF Research Database (Denmark)

    Drevs, T.; Kadakas, V; Lang, T.

    1999-01-01

    The age/length relationship in Baltic flounder (Platichthys flesus) was investigated by sex, based on otolith readings of 2925 individuals sampled from 11 stations along a transect from the Mecklenburg Eight to the Gulf of Finland in autumn 1994. A significant decrease in length at age was observ...

  19. Cryptic behaviour of juvenile turbot Psetta maxima L. and European flounder Platichthys flesus L

    DEFF Research Database (Denmark)

    Kristensen, Louise Dahl; Sparrevohn, Claus Reedtz; Christensen, Jens Tang

    2014-01-01

    The aim of this study was to examine the burying behaviour of hatchery-reared European flounder Platichthys flesus and turbot Psetta maxima, and whether conditioning on a sandy substrate would improve burying efficiency. Both species buried shortly after release on a sandy substrate. However...

  20. 50 CFR Figure 6 to Part 223 - TED Extension in Summer Flounder Trawl

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false TED Extension in Summer Flounder Trawl 6 Figure 6 to Part 223 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS THREATENED MARINE AND ANADROMOUS SPECIES Pt. 223, Fig. 6 Figure 6 to Part 223—TED...

  1. Receptivity of winter flounder larvae to artificial diet from the yolk-sac stage to metamorphosis

    DEFF Research Database (Denmark)

    Butts, Ian; Ben Khemis, I.; Litvak, Matthew Kenneth

    2015-01-01

    In the period from 4 days post-hatching to metamorphosis, winter flounder that were naïve to artificial feed were exposed to an artificial diet and allowed to forage for 8 min. The presence or absence of artificial diet in the gut was used as an indicator of acceptance. The relationship between...

  2. Stable carbon and oxygen isotope ratios of winter flounder otoliths assess connectivity between juvenile and adult habitats

    Science.gov (United States)

    Winter flounder populations (Pseudopleuronectes americanus) have significantly declined in recent years along the Rhode Island, USA coastline. The reasons for this decline are not completely clear; however, habitat loss may be a factor. Therefore, knowledge of connectivity betwee...

  3. Saptial and Temporal in Stable Carbon and Oxygen Isotope Ratios of Juvenile Winter Flounder Otoliths From Selected Nursery Areas

    Science.gov (United States)

    Winter flounder (Pseudopleuronectes americanus) populations have supported large commercial and recreational fisheries along the coast of New England. In recent years, however, the population of this important species has declined precipitously in some areas, especially Narragan...

  4. 76 FR 82189 - Fisheries of the Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries...

    Science.gov (United States)

    2011-12-30

    ..., 200,000 lb (90,718 kg) of butterfish, and 200,000 lb (90,718 kg) of bluefish. Project awards are... species, including summer flounder, scup, black sea bass, longfin squid, butterfish, and Atlantic bluefish...

  5. Purification and characterization of cathepsin L in arrowtooth flounder (Atheresthes stomias) muscle.

    Science.gov (United States)

    Visessanguan, Wonnop; Benjakul, Soottawat; An, Haejung

    2003-03-01

    A predominant, heat-activated proteinase in muscle extract of arrowtooth flounder (Atheresthes stomias) was purified to 55-fold by heat treatment, followed by a series of chromatographic separations. The apparent molecular mass of the purified enzyme was 27 kDa by size exclusion chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The proteinase had high affinity and activity toward Z-Phe-Arg-NMec with K(m) and k(cat) values of 8.2 microM and 12.2/s, respectively. Activity was inhibited by sulfhydryl reagents and activated by reducing agents. The purified proteinase displayed optimal activity at pH 5.0-5.5 and 60 degrees C, respectively. Consistent with the properties of proteases from other species, the heat-activated proteinase in arrowtooth flounder can be identified as cathepsin L.

  6. Inhibiting roles of melanin-concentrating hormone for skin pigment dispersion in barfin flounder, Verasper moseri.

    Science.gov (United States)

    Mizusawa, Kanta; Kobayashi, Yuki; Sunuma, Toshikazu; Asahida, Takashi; Saito, Yumiko; Takahashi, Akiyoshi

    2011-03-01

    Barfin flounders change their surface color pattern to match their background. We have reported evidence of the association between hormones and body color changes in this fish. First, bolus intraperitoneal injection with melanin-concentrating hormone (MCH) immediately turned the skin color pale, while injection with melanocyte-stimulating hormone (MSH) did not change the skin color. Second, gene expression levels of MCH change in response to background color, while those of MSH do not. We also reported the expression of an MCH receptor gene (Mch-r2) in the skin of this fish. In this study, we aimed to further evaluate the roles of MCH in skin color change. First, long-term adaptation of adult barfin flounder to black or white background colors induced significantly different pigment migration patterns in both melanophores and xanthophores (Ppigment-dispersing activity of MSH in a similar manner. Finally, we identified transcripts of Mch-r2 in cells isolated from both melanophores and xanthophores. Taken together, the evidence suggests that MCH aggregates pigments via MCH-R2 in concert with the nervous system by overcoming the melanin-dispersing activities of MSH in barfin flounder. Copyright © 2010 Elsevier Inc. All rights reserved.

  7. A new PCR-based method shows that blue crabs (Callinectes sapidus (Rathbun)) consume winter flounder (Pseudopleuronectes americanus (Walbaum)).

    Science.gov (United States)

    Collier, Jackie L; Fitzgerald, Sean P; Hice, Lyndie A; Frisk, Michael G; McElroy, Anne E

    2014-01-01

    Winter flounder (Pseudopleuronectes americanus) once supported robust commercial and recreational fisheries in the New York (USA) region, but since the 1990s populations have been in decline. Available data show that settlement of young-of-the-year winter flounder has not declined as sharply as adult abundance, suggesting that juveniles are experiencing higher mortality following settlement. The recent increase of blue crab (Callinectes sapidus) abundance in the New York region raises the possibility that new sources of predation may be contributing to juvenile winter flounder mortality. To investigate this possibility we developed and validated a method to specifically detect winter flounder mitochondrial control region DNA sequences in the gut contents of blue crabs. A survey of 55 crabs collected from Shinnecock Bay (along the south shore of Long Island, New York) in July, August, and September of 2011 showed that 12 of 42 blue crabs (28.6%) from which PCR-amplifiable DNA was recovered had consumed winter flounder in the wild, empirically supporting the trophic link between these species that has been widely speculated to exist. This technique overcomes difficulties with visual identification of the often unrecognizable gut contents of decapod crustaceans, and modifications of this approach offer valuable tools to more broadly address their feeding habits on a wide variety of species.

  8. Cytological-cytogenetic analyses of winter flounder embryos collected from the Benthos at the barge North Cape oil spill

    Energy Technology Data Exchange (ETDEWEB)

    Hughes, J B [Milford Laboratory, CT (United States). Northeast Fisheries Science Center

    1999-01-01

    The oil barge North Cape ran aground on Moonstone Beach in Rhode Island on January 19, 1996, spilling 828 000 gallons (US) of a combination of diesel and home heating oils. Samples of winter flounder embryos were collected from salt ponds in the area of the spill using an epibenthic sled. An examination of the field-sampled embryos based on gross morphology, levels of moribund embryos, mitotic index, and chromosomal anomalies found that a significant number exhibited high levels of one or more of these conditions when compared with flounder embryos raised under laboratory-controlled conditions. No chorion damage was noted in embryos collected from the field nor were there any significant findings of lordosis or scoliosis. The cumulative impact on winter flounder embryos of North Cape oil exposure was an estimated 51% reduction in the number of embryos surviving to the larval stage. (author)

  9. 77 FR 24151 - Fisheries of the Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries...

    Science.gov (United States)

    2012-04-23

    ... necessary to ensure the three species are not overfished or subject to overfishing in 2012. This final rule... as follows: Summer Flounder The updated stock assessment overfishing limit (OFL) is 31,588,000 lb (14... ratio is 1.01. Applying the Council's risk policy results in an overfishing risk tolerance (P*) of 0.40...

  10. First report of piscine nodavirus infecting wild winter flounder Pleuronectes americanus in Passamaquoddy Bay, New Brunswick, Canada.

    Science.gov (United States)

    Barke, Duane E; MacKinnon, Ann-Margaret; Boston, Linda; Burt, Michael D B; Cone, David K; Speare, David J; Griffiths, Steve; Cook, Marcia; Ritchie, Rachael; Olivier, Gilles

    2002-05-10

    Piscine nodaviruses (Betanodaviridae) are frequently reported from a variety of cultured and wild finfishes. These non-enveloped, single-stranded RNA virions cause viral encephalopathy and retinopathy (VER), also known as viral nervous necrosis (VNN) or fish encephalitis. Recently, nodavirus infections have posed serious problems for larval and juvenile cultured halibut Hippoglossus hippoglossus in Norway and Scotland. To date, no such viruses have been described from any cultured or wild pleuronectid in Atlantic Canada. Obviously, there exists a need to survey wild populations of pleuronectids to assess the risk of potential transfer of nodavirus from wild to caged fishes. This paper presents the results of monthly surveys (April 2000 to March 2001) of viruses from wild winter flounder Pleuronectes americanus collected from Passamaquoddy Bay, New Brunswick, Canada. Tissue samples from wild flounder were screened initially on commercial cell lines (EPC, SSN-1, SHK and CHSE-214) for any evidence of cytopathic effect (CPE). After confirmation of CPE, nodavirus identification was achieved using reverse transcription polymerase chain reaction (RT-PCR) analysis. We detected nodavirus from only 1 out of 440 flounder (0.23%) examined. This is the first report of piscine nodavirus isolated from wild winter flounder in Atlantic Canada, and although this prevalence may seem low, we discuss the implications of this finding for Canada's emerging halibut aquaculture industry.

  11. A Case Study of the Effects of Dredging in Narrgansett Bay (RI, USA) on Winter Flounder Eggs

    Science.gov (United States)

    Seasonal dredging constraints have been established in several northeastern U.S. estuaries with the intent to protect winter flounder (Pseudopleuronectes americanus). A major source of concern is impacts on demersal eggs due to elevated sedimentation rates during the winter-earl...

  12. 77 FR 68723 - Fisheries of the Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries...

    Science.gov (United States)

    2012-11-16

    ... flounder, scup, black sea bass, longfin squid, butterfish, and Atlantic bluefish fisheries. If the total... of butterfish; and 715,830 lb of bluefish. For 2014, projects may collectively be awarded up to 587... Atlantic bluefish, and assessment-quality data for weakfish, Atlantic croaker, spot, several skate and ray...

  13. 75 FR 70192 - Fisheries of the Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries...

    Science.gov (United States)

    2010-11-17

    ... squid, butterfish, and Atlantic bluefish fisheries. If the total amount of RSA is not awarded, NMFS will...; 727,527 lb (330 mt) of Loligo squid; 818,790 lb (371 mt) of bluefish; and 33,069 lb (15 mt) of... stock assessment data for summer flounder, scup, black sea bass, Loligo squid, butterfish, bluefish...

  14. Clonal xenobiotic resistance during pollution-induced toxic injury and hepatocellular carcinogenesis in liver of female flounder (Platichthys flesus (L.))

    NARCIS (Netherlands)

    Koehler, Angela; Alpermann, Tilmann; Lauritzen, Bjarne; van Noorden, Cornelis J. F.

    2004-01-01

    Juvenile and adult female flounder (Platichthys flesus (L.)) were caught either in the estuary of the most polluted European river, the Elbe, or as controls in a reference site to study pollution-induced xenobiotic resistance in their livers in relation to pathological alterations. In juvenile fish,

  15. Glucose-6-phosphate dehydrogenase: the key to sex-related xenobiotic toxicity in hepatocytes of European flounder (Platichthys flesus L.)?

    NARCIS (Netherlands)

    Winzer, Katja; van Noorden, Cornelis J. F.; Köhler, Angela

    2002-01-01

    The role of glucose-6-phosphate dehydrogenase (G6PDH) in oxidative stress responses was investigated in isolated intact living hepatocytes of immature female and male European flounder (Platichthys flesus L.) because it is the major provider of NADPH needed as reducing power for various

  16. Fecundity regulation in relation to habitat utilisation of two sympatric flounder (Platichtys flesus) populations in the brackish water Baltic Sea

    DEFF Research Database (Denmark)

    Nissling, Anders; Thorsen, Anders; da Silva, Filipa F.G.

    2015-01-01

    Two populations of flounder (Platichtys flesus) with different life history traits inhabit the brackish water Baltic Sea. Both types share feeding areas in coastal waters during summer-autumn but utilise different habitats for spawning in spring, namely offshore spawning with pelagic eggs and coa...

  17. Osteología craneal comparada de tres especies de lenguado del género Paralichthys (Pleuronectiformes, Paralichthyidae del Atlántico suroccidental Comparative cranial osteology of three species of the flatfish genus Paralichthys (Pleuronectiformes, Paralichthyidae from the southwestern Atlantic

    Directory of Open Access Journals (Sweden)

    JUAN M. DÍAZ DE ASTARLOA

    2005-09-01

    Full Text Available Se realiza un análisis osteológico comparativo del esqueleto craneal en Paralichthys isosceles, P. orbignyanus y P. patagonicus, tres especies de lenguados presentes en el Atlántico suroccidental. Se describen las características de los componentes óseos y se detallan las diferencias morfológicas y morfométricas encontradas, con el fin de aportar elementos diagnósticos para la identificación taxonómica de las tres especies de Paralichthys. Se utilizaron técnicas de desarticulación de esqueletos, tanto en ejemplares frescos congelados, como en especímenes conservados en líquidos fijadores. También fueron usadas técnicas de clareado y tinción diferencial para ejemplares de pequeño tamaño. Se observaron potenciales características diagnósticas para la discriminación de las especies en los huesos paretmoides derecho, lacrimal izquierdo, anguloarticular, mesopterigoides, los otolitos y el número de dientes del dentario. Se discuten las relaciones entre los huesos y se comparan sus características con las halladas en otras especies del género Paralichthys, en particular, y en otros Pleuronectiformes en generalAn osteological analysis of the cranial skeleton of the southwestern Atlantic flatfish species Paralichthys isosceles, P. orbignyanus and P. patagonicus was carried out based on comparative morphology and morphometrics of the bones. The objective was to provide osteological evidence of diagnostic value for a correct identification of the flatfish species. Methods for preparing disarticulated skeletons were used for both dissected fresh-frosen and alcohol-preserved specimens. Also methods of clearing and staining for bone and cartilage were applied for small fish specimens. Potentially diagnostic features were found to distinguish among species in the right parethmoid, left lachrymal, angulo-articular, mesopterygoid, the otoliths, and the number of dentary teeth. Relationships between bones are discussed and bone

  18. Cyclic volatile methylsiloxane bioaccumulation in flounder and ragworm in the Humber Estuary.

    Science.gov (United States)

    Kierkegaard, Amelie; van Egmond, Roger; McLachlan, Michael S

    2011-07-15

    Cyclic volatile methylsiloxanes are being subjected to regulatory scrutiny as possible PBT chemicals. The investigation of bioaccumulation has yielded apparently contradictory results, with high laboratory fish bioconcentration factors on the one hand and low field trophic magnification factors on the other. In this study, octamethylcyclotetrasiloxane (D4), decamethylcyclopentasiloxane (D5), and dodecamethylcyclohexasiloxane (D6) were studied along with polychlorinated biphenyls (PCBs) in sediments, ragworm, and flounder from six sites in the Humber Estuary. Bioaccumulation was evaluated using multimedia bioaccumulation factors (mmBAFs) which quantified the fraction of the contaminant present in the aquatic environment that is transferred to the biota. PCB 180, a known strongly bioaccumulative chemical, was used as a benchmark. The mean mmBAF of D5 was about twice that of PCB 180 in both polycheates and flounder, while for D4 it was 6 and 14 times higher, respectively. The mmBAF of D6 was a factor 5-10 lower than that of PCB180. The comparatively strong multimedia bioaccumulation of D4 and D5, even in the absence of biomagnification, was explained by both compounds having a >100 times stronger tendency to partition into lipid rather than into organic carbon, while PCB 180 partitions to a similar extent into both matrices.

  19. Ichthyophonus irregularis sp. nov. from the yellowtail flounder Limanda ferruginea from the Nova Scotia shelf.

    Science.gov (United States)

    Rand, T G; White, K; Cannone, J J; Gutell, R R; Murphy, C A; Ragan, M A

    2000-05-25

    A previously described unusual form of the protistan parasite Ichthyophonus, differing in morphological and developmental features from I. hoferi sensu Plehn & Mulsow, was recovered from yellowtail flounder Limanda ferruginea Storer from the Brown's Bank area of the Nova Scotia shelf. The nuclear gene encoding the rRNA of the small ribosomal subunit was amplified from this unusual form of Ichthyophonus using the polymerase chain reaction, sequenced and aligned with other eukaryote small subunit (ssu)-rDNAs. Inferred phylogenetic trees clearly show that its ssu-rDNA is distinct from those of 2 isolates of I. hoferi sensu Plehn & Mulsow from different hosts and geographical locations (herring in the North Sea, and yellowtail flounder from the Nova Scotia shelf). We consider the unusual form to be a separate species, I. irregularis. The occurrence of a second, distinct type of Ichthyophonus within a single host species raises the possibility that ichthyophoniasis could be produced by different (although related) pathogens, and in some cases, by concurrent infections of the two.

  20. Fecundity regulation in relation to habitat utilisation of two sympatric flounder (Platichtys flesus) populations in the brackish water Baltic Sea

    Science.gov (United States)

    Nissling, Anders; Thorsen, Anders; da Silva, Filipa F. G.

    2015-01-01

    Two populations of flounder (Platichtys flesus) with different life history traits inhabit the brackish water Baltic Sea. Both types share feeding areas in coastal waters during summer-autumn but utilise different habitats for spawning in spring, namely offshore spawning with pelagic eggs and coastal spawning with demersal eggs respectively. Fecundity regulation by atresia was assessed as prevalence (portion of fish with atresia) and intensity (calculated as the average intensity of atresia in these fish) during the reproductive cycle following start of gonad development in the autumn up to spawning in spring, and evaluated in relation to fish condition (Fulton's condition factor reflecting energy reserves of the fish) and feeding incidence of the respective population. Peaking in winter (December-February), fecundity regulation was significantly higher for coastal spawning flounder than for flounder spawning offshore. For coastal spawners, the prevalence was 45-90% with an intensity of 6.4-9.3% vs. 0-25% and an intensity of 2.1-3.4% for offshore spawners during winter. Further, fecundity regulation ceased prior to spawning for offshore spawners but continued for coastal spawners. For coastal spawners, the prevalence was 12-29% and an intensity of 2.5-6.1% during spawning. The change in fish condition was strongly related to feeding incidence and differed between populations. As feeding ceased, condition of offshore spawners decreased during winter up to spawning, whereas condition of coastal spawners decreased during autumn but was maintained as feeding started again prior to spawning. Thus, habitat utilisation according to spawning strategy affects the timing of fecundity down-regulation reflecting availability of resources, namely limited food resources in deep areas and higher availability in coastal areas. Offshore spawning flounder display characteristics typical for a capital spawner with ceasing of feeding and oocyte down-regulation well before spawning

  1. Development of practical diet for aquaculture of Japanese flounder; Gyofun wo sakutenshita hirameyo shiryo no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    Kikuchi, K.; Furuta, T.

    2000-07-01

    Feeding experiments were conducted to examine the potential use of defatted soybean meal (SBM) in combinations with blood meal (BM), corn gluten meal (CGM) and freeze dried meat of blue mussel (BMM) as a partial replacement of fish meal (FM) in the diet of Japanese flounder. Availability of CGM in combinations with BM and/or BMM was also examined. Juvenile fish of about 4 g in initial body weight were fed diets to satiation twice daily, 6 days per week for 8 weeks at 20 degree C in both feeding experiments. Weight gain of fish fed diet replacing 45% FM protein with SBM was not different from that of fish fed the control diet containing FM as a protein source, however, feed efficiency (FE) in fish fed the SBM diet was significantly lower. Growth and feed performance of fish fed diet replacing 45% FM protein with SBM and BM were comparable to those of fish the control diet. Inclusion of 5% BMM for equal amount of SBM in the SBM-BM diet improved the growth and FE of the flounder remarkably and both values were significantly higher than those in the control. Replacing 55% of FM protein with SBM and CGM or SBM and BM resulted in poor growth performance. There were not marked differences in hematological characteristics together with proximate composition of the whole body of cultured fish fed the dietary treatments. Weight gain and FE of fish fed diet replacing 40% FM protein with CGM were inferior to those of fish fed the control diet containing FM as a protein source. Replacement of 40% FM protein with CGM and BM made those parameters worse. Inclusion of BMM in CGM-BM diet did not improve the growth of fish. Adverse effects of replacing FM protein with CGM and/or CGM and BM were stronger with increasing replacement level. These results indicate that SBM is highly available as alternative protein source for fish meal in the diet of Japanese flounder. SBM in combination with BM successfully replace about 45% of FM protein in the diet without supplemental amino acids

  2. Survival of Anisakis simplex in arrowtooth flounder (Atheresthes stomia) during frozen storage.

    Science.gov (United States)

    Adams, Ann M; Ton, My N; Wekell, Marleen M; MacKenzie, Alan P; Dong, Faye M

    2005-07-01

    Survival of naturally occurring larvae of Anisakis simplex in fresh arrowtooth flounder (Atheresthes stomia) was determined after storage for specified periods at four freezing temperatures. All larvae were killed by 96, 60, 12, and 9 h at temperatures of -15, -20, -30, and -40 degrees C, respectively. The average percentages of live larvae per fillet at the next shortest holding time were as follows: 72 h at -15 degrees C, 0 to 3%; 48 h at -20 degrees C, 11 to 30%; 9 h at -30 degrees C, 5%; and 6 h at -40 degrees C, 0 to 3%. Larval survival was directly related to fillet thickness or weight (P < or = 0.05). Larval death was directly correlated to freezing temperatures. Holding time necessary to kill larval nematodes decreased as storage temperature decreased.

  3. Properties of protein powders from arrowtooth flounder (Atheresthes stomias) and herring (Clupea harengus) byproducts.

    Science.gov (United States)

    Sathivel, Subramaniam; Bechtel, Peter J; Babbitt, Jerry; Prinyawiwatkul, Witoon; Negulescu, Ioan I; Reppond, Kermit D

    2004-08-11

    Functional, nutritional, and thermal properties of freeze-dried protein powders (FPP) from whole herring (WHP), herring body (HBP), herring head (HHP), herring gonad (HGP), and arrowtooth flounder fillets (AFP) were evaluated. The FPP samples have desirable nutritional and functional properties and contained 63-81.4% protein. All FPP samples had desirable essential amino acid profiles and mineral contents. The emulsifying and fat adsorption capacities of all FPP samples were higher than those of soy protein concentrate. The emulsifying stability of WHP was lower than that of egg albumin but greater than that of soy protein concentrate. Thermal stability of the FPP samples is in the following order: HGP > HBP > WHP > HHP > AFP.

  4. Differentiation of chondrocytes and scleroblasts during dorsal fin skeletogenesis in flounder larvae.

    Science.gov (United States)

    Suzuki, Tohru; Haga, Yutaka; Takeuchi, Toshio; Uji, Susumu; Hashimoto, Hisashi; Kurokawa, Tadahide

    2003-01-01

    In teleosts, the embryonic fin fold consists of a peridermis, an underlying epidermis and a small number of mesenchymal cells. Beginning from such a simple structure, the fin skeletons, including the proximal and distal radials and lepidotrichia (finrays), develop in the dorsal fin fold at the larval stage. Their process of skeletogenesis and embryonic origin are unclear. Using flounder larvae, we report the differentiation process for chondrocytes and scleroblasts prior to fin skeletogenesis and the effects of retinoic acid (RA) on it. In early larvae, the mesenchymal cells grow between the epidermis and spinal cord to form a line of periodical condensations, which are proximal radial primordia, to produce chondrocytes. The prescleroblasts, which ossify the proximal radial cartilages, differentiate in the mesenchymal cells remaining between the cartilages. Then, mesenchymal condensations occur between the distal ends of the proximal radials, forming distal radial primordia, to produce chondrocytes. Simultaneously, condensations occur between the distal radial primordia and peridermis, which are lepidotrichia primordia, to produce prescleroblasts. Exogenous RA specifically inhibits the mesenchymal condensation prior to the proximal radial formation together with the down-regulation of sonic hedgehog (shh) and patched (pta) expression, resulting in the loss of proximal radials. Thus, it was indicated that differentiation of the precursor cells of radials and lepidotrichia begins in the proximal part of the fin fold and that the initial mesenchymal condensation prior to the proximal radial formation is highly susceptible to the effects of RA. Lepidotrichia formation does not occur where proximal radials are absent, indicating that lepidotrichia differentiation requires interaction with the radial cartilages. To examine the suggestion that neural crest cells contribute to the medial fin skeletons, we localized the HNK-1 positive cells in flounder embryos and slug and

  5. Population ecology and habitat preferences of juvenile flounder Platichthys flesus (Actinopterygii: Pleuronectidae) in a temperate estuary

    Science.gov (United States)

    Souza, Allan T.; Dias, Ester; Nogueira, Ana; Campos, Joana; Marques, João C.; Martins, Irene

    2013-05-01

    The European flounder Platichthys flesus is a widely distributed epibenthic species and an important component of demersal fish assemblages in the European Atlantic coastal waters. In Portuguese estuaries, this species reaches high densities, especially in Minho estuary (NW Iberian Peninsula, Europe), potentially playing an important role in the system's ecology. In this context, the population structure, production and the habitat use of juvenile P. flesus were investigated. Sampling took place monthly, from February 2009 until July 2010 along the entire estuarine gradient (5 sampling stations distributed in the first 29 km from the river mouth, with S1 located near the river mouth, S2 inside a salt marsh, S3 in a salinity transition zone, while S4 and S5 were located in the upper estuary). Flounder's density varied significantly among sampling stations and seasons (two-way PERMANOVA: p PERMANOVA: p < 0.001), with larger fishes being found in S1 during the autumn (168.50 ± 59.50 mm) and the smallest in S4 during the spring (33.80 ± 3.12 mm). Size classes associated differently with environmental variables, with larger juveniles being more abundant in the downstream areas of the estuary, whereas smaller juveniles were related to higher water temperatures, suggesting a habitat segregation of P. flesus of different sizes. The fish condition of P. flesus in Minho estuary was higher than in other systems, probably due to the dominance of juveniles on the population. Also, the densities found in this estuary were up to 32 times higher than in other locations, suggesting that Minho estuary is an important nursery area for the species. The estimated secondary production of P. flesus was lower than previous studies acknowledged in the system (0.037 g.WWm- 2.year- 1), indicating that the production estimates of this species in estuaries can vary considerably depending on of several factors such as the sampling year and strategy, population and fish size.

  6. Assessing power plant impacts on fish populations at Northeast Utilities sites: winter flounder studies at Millstone Nuclear Power Station

    International Nuclear Information System (INIS)

    Lorda, E.; Danila, D.J.; Miller, J.D.; Bireley, L.E.; Jacobsen, P.M.

    1987-01-01

    An historical view is presented of the various impact assessment approaches used to study the winter flounder, including efforts to identify and quantify compensation and to model its population dynamics. This review illustrates the need for unbiased estimates of basic life history parameters and power plant related mortalities if compensatory mechanisms are to be understood and if impact assessments are to be meaningful. 67 references, 19 figures, 10 tables

  7. Control strategy for viral diseases of salmonid fish, flounders and shrimp at hatchery and seed production facility in Japan

    OpenAIRE

    Yoshimizu, Mamoru

    2009-01-01

    Salmonid fish are important species for hatchery reared and released fish. Flounders and shrimp are also important species for seed production and sea-farming in Japan. Viral disease is one of the limitations of successful propagation of these species. Methods currently used to control viral diseases are 1) hygiene and sanitation in facilities, 2) disinfection of rearing and waste water using U. V. irradiation, ozonization and electrolyzation, 3) selection of pathogen-free brood stock by cell...

  8. Relationship between growth of marbled flounder (Pleuronectes yokohamae) and Cs concentration in their muscle determined by ICP-MS

    International Nuclear Information System (INIS)

    Isoyama, Naohiko; Oikawa, Shinji; Misonoo, Jun

    2008-01-01

    For the purpose of marine environmental radioactivity monitoring, stable Cs and 137 Cs concentrations were obtained from 214 marbled flounder (Pleuronectes yokohamae) samples collected from off the coast of Ibaraki prefecture in Japan in 2005 and 2006. Prior to the investigation, an analytical method for the determination of Cs in muscle of marbled flounder samples by inductively coupled plasma mass spectrometry (ICP-MS) was seriously considered from the view point of sample preparation (wet way) to a Cs measurement. Since major problems in determining Cs by the ICP-MS is the interference of matrix materials and molecular ions on Cs peak area (m/z 133), a known amount of In (m/s 115) was added to the sample solutions as an internal standard. To check the accuracy of the determination, duplication analysis and standard reference materials were used. Analytical results of Cs in muscles samples by ICP-MS agreed well with duplicated samples, and standard reference materials. The standard length and body weight were obtained from a total of 214 flounder samples. The stable Cs, stable K and 137 Cs concentrations in muscle were determined by the ICP-MS, flame photometry and radiochemical analysis, respectively. The mean standard length correlates closely with the mean body weight. The stable Cs concentration was in proportion to the body weight, also, the 137 Cs concentration correlated closely with stable Cs concentration in muscle. On the other hand, a stable K concentration was found to be uniform, and unrelated with 137 Cs and stable Cs concentrations. The specific activities, the ratio of 137 Cs/stable Cs in muscle, was found to be 5.5 - 6.7 with an average of 6.2 ± 0.35 (SD) among five different size groups of the standard length of marbled flounder samples. From these results, ICP-MS provided useful tools for the determination of a trace amount of Cs in muscle samples, combined with wet way pretreatment techniques. In addition, marbled flounder proved to be a

  9. Relationship between biomarker responses and contaminant concentration in selected tissues of flounder (Platichthys flesus from the Polish coastal area of the Baltic Sea

    Directory of Open Access Journals (Sweden)

    Magdalena Podolska

    2008-09-01

    Full Text Available Previous studies in the Gulf of Gdańsk discussed the responses of selected enzymatic biomarkers to the contaminant gradient in fish and mussels. In the present study, flounder muscle and liver tissues were analyzed for polychlorinated biphenyls (PCB congeners: 28, 52, 101, 118, 138, 153 and 180, organochlorine pesticides (HCHs, HCB and DDTs, and trace metals (Pb, Cd, Zn, Cu, Hg, Cr. An attempt was made to identify the relationship between the measured enzymatic biomarker responses (cholinesterases, malic enzyme, isocitrate dehydrogenase, glutathione S-transferase and contaminant concentrations in selected flounder tissues. The observed differences in enzymatic biomarker levels suggest that chronic exposure to low-concentration mixtures of contaminants may be occurring in the studied area. However, no conclusive evidence was found of a clear link between the biomarker responses and contaminant concentrations in flounder tissues.

  10. Effects of cooking on levels of PCBs in the fillets of winter flounder (Pseudopleuronectes americanus)

    Energy Technology Data Exchange (ETDEWEB)

    Poston, T.M. [Pacific Northwest Lab., Richland, WA (United States); Durell, G.S.; Koczwara, G.; Spellacy, A.M. [Battelle Ocean Sciences, Duxbury, MA (United States)

    1995-08-01

    The Pacific Northwest Laboratory and Battelle Ocean Sciences performed a study to determine the effect of cooking on polychlorinated biphenyl (PCB) levels in the fillets of winter flounder (Pseudopleuronectes americanus). Broiling, pan frying, and deep frying in oil were tested on fillets from 21 fish collected from New Bedford Harbor, Massachusetts, on February 21, 1991. The evaluation involved estimating the change in PCB concentrations using a mass-balance approach that factored the change in fillet weight resulting from cooking with the changes in PCB concentration expressed on a precooked wet-weight basis. Deep frying in oil resulted in a 47% reduction in total PCB levels in fillet tissue. Additionally, deep frying caused a 40% reduction in fillet mass. Pan frying and broiling resulted in statistically in insignificant increases in total PCB levels of 15% and 17%, respectively. Fillet mass reductions resulting from pan frying and broiling were 7% and 15%, respectively. The effects of cooking on 18 individual congeners generally paralleled the results observed for total PCB. All 18 congeners were significantly reduced by deep frying. Congener Cl{sub 2}(08) also was significantly reduced by either pan frying. Congeners Cl{sub 5}(105) and Cl{sub 5}(118) showed apparent significant increases in concentrations following pan frying. Congeners Cl{sub 5}(105), Cl{sub 5}(118), and C1{sub 6}(138) showed significant increases in concentration following broiling.

  11. Developmental toxicity in flounder embryos exposed to crude oils derived from different geographical regions.

    Science.gov (United States)

    Jung, Jee-Hyun; Lee, Eun-Hee; Choi, Kwang-Min; Yim, Un Hyuk; Ha, Sung Yong; An, Joon Geon; Kim, Moonkoo

    2017-06-01

    Crude oils from distinct geographical regions have distinct chemical compositions, and, as a result, their toxicity may be different. However, developmental toxicity of crude oils derived from different geographical regions has not been extensively characterized. In this study, flounder embryos were separately exposed to effluents contaminated by three crude oils including: Basrah Light (BLO), Pyrenees (PCO), and Sakhalin Vityaz (SVO), in addition to a processed fuel oil (MFO-380), to measure developmental toxicity and for gene expressions. Each oil possessed a distinct chemical composition. Edema defect was highest in embryos exposed to PCO and MFO-380 that both have a greater fraction of three-ring PAHs (33% and 22%, respectively) compared to BLO and SVO. Observed caudal fin defects were higher in embryos exposed to SVO and MFO-380, which are both dominated by naphthalenes (81% and 52%, respectively). CYP1A gene expressions were also highest in embryos exposed to SVO and MFO-380. Higher incidence of cardiotoxicity and lower nkx 2.5 expression were detected in embryos exposed to PCO. Unique gene expression profiles were observed in embryos exposed to crude oils with distinct compositions. This study demonstrates that crude oils of different geographical origins with different compositional characteristics induce developmental toxicity to different degrees. Copyright © 2017 Elsevier Inc. All rights reserved.

  12. Tyrosine transport in winter flounder intestine: Interaction with Na+-K+-2Cl- cotransport

    International Nuclear Information System (INIS)

    Musch, M.W.; McConnell, F.M.; Goldstein, L.; Field, M.

    1987-01-01

    Tyrosine absorption across the brush border of the intestinal epithelium of the winter flounder Pseudopleuronectes americanus was studied in Ussing chambers modified to determine early rates of uptake. At 0.1 mM tyrosine, the 4-min rate of uptake (influx) of tyrosine across the brush border averaged 37.5 nmol·cm -2 ·h -1 . Omission of Na decreased influx by 60%, indicting that tyrosine influx occurs, at least in part, by a Na-coupled process. Ouabain inhibited influx by 80%. Inhibition of brush border Na + -K + -2Cl - cotransport by bumetanide, 8-bromo-cyclic GMP, or Cl replacement stimulated tyrosine influx 2.5- to 4-fold. However, atriopeptin III, which also inhibits Na + -K + -2Cl - cotransport, did not stimulate tyrosine influx. Cyclic AMP, which does not appear to inhibit ion cotransport, did not stimulate tyrosine influx. Both cyclic GMP and bumetanide also stimulated the net mucosa-to-serosa tyrosine flux (43 and 29%, respectively) and increased the cellular concentration of tyrosine by 50%. Thus tyrosine's influx is increased to a greater extent than is its transmural flux or its cellular concentration, suggesting that the main change occurs at the brush border and represents large increases in both influx and efflux of tyrosine across this membrane

  13. Tissue specific haemoglobin gene expression suggests adaptation to local marine conditions in North Sea flounder (Platichthys flesus L.)

    DEFF Research Database (Denmark)

    Larsen, P.F.; Eg Nielsen, Einar; Hansen, M.M.

    2013-01-01

    Recent genetic analyses of candidate genes and gene expression in marine fishes have provided evidence of local adaptation in response to environmental differences, despite the lack of strong signals of population structure from conventional neutral genetic markers. In this study expression...... in flounder. In gill tissue a plastic response to salinity treatments was observed with general up-regulation of these genes concomitant with higher salinity. For liver tissue a population specific expression differences was observed with lower expression at simulated non-native compared to native salinities...... in high gene flow marine fishes. © 2013 The Genetics Society of Korea...

  14. Genetic diversity in two Japanese flounder populations from China seas inferred using microsatellite markers and COI sequences

    Science.gov (United States)

    Xu, Dongdong; Li, Sanlei; Lou, Bao; Zhang, Yurong; Zhan, Wei; Shi, Huilai

    2012-07-01

    Japanese flounder is one of the most important commercial species in China; however, information on the genetic background of natural populations in China seas is scarce. The lack of genetic data has hampered fishery management and aquaculture development programs for this species. In the present study, we have analyzed the genetic diversity in natural populations of Japanese flounder sampled from the Yellow Sea (Qingdao population, QD) and East China Sea (Zhoushan population, ZS) using 10 polymorphic microsatellite loci and cytochrome c oxidase subunit I (COI) sequencing data. A total of 68 different alleles were observed over 10 microsatellite loci. The total number of alleles per locus ranged from 2 to 9, and the number of genotypes per locus ranged from 3 to 45. The observed heterozygosity and expected heterozygosity in QD were 0.733 and 0.779, respectively, and in ZS the heterozygosity values were 0.708 and 0.783, respectively. Significant departures from Hardy-Weinberg equilibrium were observed in 7 of the 10 microsatellite loci in each of the two populations. The COI sequencing analysis revealed 25 polymorphic sites and 15 haplotypes in the two populations. The haplotype diversity and nucleotide diversity in the QD population were 0.746±0.072 8 and 0.003 34±0.001 03 respectively, and in ZS population the genetic diversity values were 0.712±0.047 0 and 0.003 18±0.000 49, respectively. The microsatellite data ( F st =0.048 7, P <0.001) and mitochondrial DNA data ( F st =0.128, P <0.001) both revealed significant genetic differentiation between the two populations. The information on the genetic variation and differentiation in Japanese flounder obtained in this study could be used to set up suitable guidelines for the management and conservation of this species, as well as for managing artificial selection programs. In future studies, more geographically diverse stocks should be used to obtain a deeper understanding of the population structure of Japanese

  15. Quantitative cytochemical analysis of glucose-6-phosphate dehydrogenase activity in living isolated hepatocytes of European flounder for rapid analysis of xenobiotic effects

    NARCIS (Netherlands)

    Winzer, K.; van Noorden, C. J.; Köhler, A.

    2001-01-01

    There is a great need for rapid but reliable assays to determine quantitatively effects of xenobiotics on biological systems in environmental research. Hepatocytes of European flounder are sensitive to low-dose toxic stress. Glucose-6-phosphate dehydrogenase (G6PDH) is the major source of NADPH in

  16. Trends in nitrogen isotope ratios of juvenile winter flounder reflect changing nitrogen inputs to Rhode Island, USA estuarine systems.

    Science.gov (United States)

    Pruell, Richard J; Taplin, Bryan K; Miller, Kenneth M

    2017-05-15

    Nitrogen isotope ratios (δ 15 N) in juvenile winter flounder, Pseudopleuronectes americanus, were used to examine changes in nitrogen inputs to several Rhode Island, USA estuarine systems. Fish were collected over two three-year periods with a ten-year interval between sampling periods (2002-2004 and 2012-2014). During that interval numerous changes to nutrient management practices were initiated in the watersheds of these estuarine systems including the upgrade of several major wastewater treatment facilities that discharge to Narragansett Bay, which significantly reduced nitrogen inputs. Following these reductions, the δ 15 N values of flounder in several of the systems decreased as expected; however, isotope ratios in fish from upper Narragansett Bay significantly increased. We believe that low δ 15 N values measured in 2002-2004 were related to concentration-dependent fractionation at this location. Increased δ 15 N values measured between 2012 and 2014 may indicate reduced fractionation or that changes in wastewater treatment processes altered the nitrogen isotopic ratios of the effluents. Published by Elsevier Ltd.

  17. Distortion of frontal bones results from cell apoptosis by the mechanical force from the up-migrating eye during metamorphosis in Paralichthys olivaceus.

    Science.gov (United States)

    Sun, Mingyan; Wei, Fen; Li, Hui; Xu, Juan; Chen, Xinye; Gong, Xiaoling; Tian, Yongsheng; Chen, Songlin; Bao, Baolong

    2015-05-01

    Craniofacial remodeling during flatfish metamorphosis, including eye migration, is perhaps the most striking example of asymmetric postembryonic development in the vertebrate world. The asymmetry of the cranium mainly results from distortion of the frontal bones, which depends on eye migration during metamorphosis. However, it is unclear how the up-migrating eye causes distortion of the frontal bones. In this study, we first show that distortion of the frontal bones during metamorphosis in Paralichthys olivaceus is the result of cell apoptosis, rather than cell autophagy or cell proliferation. Secondly, we report that cell apoptosis in the frontal bones is induced by the mechanical force transferred from the up-migrating eye. The mechanical force from the up-migrating eye signals through FAK to downstream molecules that are integrated into the BMP-2 signal pathway. Finally, it is shown that cell apoptosis in the frontal bones is activated by the intrinsic mitochondrial pathway; the extrinsic death receptor is not involved in this process. Moreover, cell apoptosis in frontal bones is not induced directly by thyroid hormones, which are thought to mediate metamorphosis in flatfishes and directly mediate cell apoptosis during amphibian metamorphosis. These findings help identify the major signaling route used during regulation of frontal bone distortion during metamorphosis in flatfish, and indicate that the asymmetry of the cranium, or at least the distortion of frontal bones, is the result of rather than the reason underlying eye migration. Copyright © 2015. Published by Elsevier Ireland Ltd.

  18. Embryonic and larval development in barfin flounder Verasper moseri (Jordan and Gilbert)

    Science.gov (United States)

    Du, Rongbin; Wang, Yongqiang; Jiang, Haibin; Liu, Liming; Wang, Maojian; Li, Tianbao; Zhang, Shubao

    2010-01-01

    side on day 50, when the average body length attained 2.5±0.18 cm, and juveniles accomplished metamorphosis to young. The embryonic and larval characters of several flounder species are compared.

  19. Survival of Anisakis simplex in microwave-processed arrowtooth flounder (Atheresthes stomias).

    Science.gov (United States)

    Adams, A M; Miller, K S; Wekell, M M; Dong, F M

    1999-04-01

    The purpose of this study was to define the relationship between survival and temperature of nematodes of the species Anisakis simplex in microwave-processed arrowtooth flounder (Atheresthes stomias). Ten fillets (each 126 to 467 g, 0.5 to 1.75 cm thick), with an average of five larvae of Anisakis simplex per fillet, were processed to target temperatures on high (100%) power using a commercial 700-W microwave oven. Fillets were neither covered nor rotated and had a temperature probe inserted to two-thirds depth into the thickest portion. After the fillet was digested using a 1% pepsin solution, the viability of nematodes was determined by viewing them under a dissecting microscope. Survival rates were 31% at 140 degrees F (60 degrees C), 11% at 150 degrees F (65 degrees C), 2% at 160 degrees F (71 degrees C), 3% at 165 degrees F (74 degrees C), and 0% at 170 degrees F (77 degrees C). Microwave processing of standardized fillet "sandwiches," 14 cm long, 4.5 cm wide, and approximately 1.75 cm high, each of which was preinoculated with 10 live nematodes, resulted in no survival at either 160 degrees F or 170 degrees F. Using ultraviolet light to detect both viable and nonviable nematodes in fillet sandwiches as an alternative method to pepsin digestion resulted in survival rates of 1% at 140 degrees F (60 degrees C), 3% at 145 degrees F (63 degrees C), and 0% at 150 degrees F (65 degrees C). Smaller fillet sandwiches, which most likely had fewer cold spots during microwave processing, required 150 degrees F (65 degrees C), whereas larger whole fillets required 170 degrees F (77 degrees C) to kill larvae of Anisakis simplex. The parasites were most likely inactivated by a thermal mechanism of microwave treatment. Damage to the nematodes was often evident from ruptured cuticles that were no longer resistant to digestive enzymes. The high hydrostatic pressure and low chloride content of the pseudocoelomic fluid probably contributed greatly to the damage incurred by the

  20. {sup 241}Pu concentrations in water, plankton and fish from the southern Baltic Sea

    Energy Technology Data Exchange (ETDEWEB)

    Struminska-Parulska, D.I.; Skwarzec, B. [Gdansk, Univ. (Poland). Facuty of Chemistry

    2013-08-01

    The aim of the work was to determine {sup 241}Pu activities in different components (water, plankton and fish) of the southern Baltic Sea ecosystem. Measurement of {sup 241}Pu in the samples was done indirectly by determining the increment in {sup 241}Am from the decay of the {beta}-emitting {sup 241}Pu in samples collected 10-15 years after the Chernobyl accident. Enhanced levels of {sup 241}Pu were observed in all analyzed Baltic samples. The highest {sup 241}Pu concentrations in fish were found in Perciformes: benthic round goby (0.863 {+-} 0.066 mBq/g ww) and pelagic perch (0.666 {+-} 0.001 mBq/g ww). Plutonium is also non-uniformly distributed in the organs and tissues of the analyzed fish; especially pelagic herring and cod as well as benthic flounder. Most of {sup 241}Pu in flounder, herring and cod is located in soft tissues, especially digestive organs (stomach, intestine, liver). The annual individual effective doses calculated on the basis of {sup 241}Pu concentrations in fish indicated that the impact of the consumption of {sup 241}Pu containing Baltic fish on the annual effective dose for a statistical inhabitant of Poland was very small. (orig.)

  1. Binding and movement of silver in the intestinal epithelium of a marine teleost fish, the European flounder (Platichthys flesus)

    DEFF Research Database (Denmark)

    Hogstrand, C.; Wood, C. M.; Bury, N.R.

    2002-01-01

    The intestine has been indicated as a site of waterborne silver toxicity in marine fish and chronic effects at the intestine have been observed at concentrations far below acutely toxic level. Thus, models of silver toxicity to marine fish need to consider the intestine as a biotic ligand....... The present study characterises binding of silver to the intestine of the European flounder (Platichthys flesus). Everted intestinal sacks were prepared and submersed in a solution mimicking the intestinal fluid of the fish at the acclimation salinity (21‰). Silver was added as 110mAgNO3 or 110mAgNO3/AgNO3...... mixtures at concentrations ranging from 1.6 to 950 nM total silver. Appearance of 110mAg was analysed in mucosal scrapings, muscle layers, and in the plasma saline on the serosal side of the intestine. The latter represented uptake into blood and other extra-intestinal compartments. Mucosal scrapings...

  2. Effect of water temperature on survival of early-life stages of marbled flounder Pseudopleuronectes yokohamae in Tokyo Bay, Japan.

    Science.gov (United States)

    Lee, Jeong-Hoon; Kodama, Keita; Oyama, Masaaki; Shiraishi, Hiroaki; Horiguchi, Toshihiro

    2017-07-01

    We investigated factors that might have disturbed the stock recovery of marbled flounder in Tokyo Bay by focusing on the early life stages. Field surveys in Tokyo Bay from 2006 to 2011 revealed that mature adult biomass increased from 2006 to 2008 and decreased thereafter. Meanwhile, larval and juvenile densities were high in 2006 and 2008 but low in other years. Discrepancies in the yearly trends of these parameters suggest that mortality during life stages between spawning and early larval phases might have affected the abundance of the subsequent life stages. Monthly mean water temperature between January and February, in which hatching and pelagic larvae occur in the bay, was lower in 2006 (8.6 °C) and 2008 (9.6 °C) than was observed in other years (10.4-11.4 °C). Significant negative correlation between water temperature and larval density implies that mortality during pre- and post-larval stages would be higher in warmer winter years (>10 °C). To test this hypothesis, we examined the effects of water temperature on mortality and development in egg and larval stages under controlled laboratory conditions. Hatching rate was high in a water temperature range of 9.2-12.7 °C (66.6-82.5%), whereas it decreased in cooler (3.7% at 5.9 °C) or warmer (33.9% at 14.8 °C) conditions. Meanwhile, days from fertilization to hatching, size of larvae at hatching and survival rate of larvae after 18 d from hatching were monotonically and significantly decreased as water temperature was elevated. Combined evidence of the field and laboratory studies suggests that a warmer reproductive season (>10 °C) might induce mortalities of marbled flounder larvae in Tokyo Bay. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Evolutionary conservation and expression of miR-10a-3p in olive flounder and rock bream.

    Science.gov (United States)

    Jo, Ara; Im, Jennifer; Lee, Hee-Eun; Jang, Dongmin; Nam, Gyu-Hwi; Mishra, Anshuman; Kim, Woo-Jin; Kim, Won; Cha, Hee-Jae; Kim, Heui-Soo

    2017-09-10

    MicroRNAs (miRNAs) are small non-coding RNAs (ncRNAs) that mainly bind to the seed sequences located within the 3' untranslated region (3' UTR) of target genes. They perform an important biological function as regulators of gene expression. Different genes can be regulated by the same miRNA, whilst different miRNAs can be regulated by the same genes. Here, the evolutionary conservation and expression pattern of miR-10a-3p in olive flounder and rock bream was examined. Binding sites (AAAUUC) to seed region of the 3' UTR of target genes were highly conserved in various species. The expression pattern of miR-10a-3p was ubiquitous in the examined tissues, whilst its expression level was decreased in gill tissues infected by viral hemorrhagic septicemia virus (VHSV) compared to the normal control. In the case of rock bream, the spleen, kidney, and liver tissues showed dominant expression levels of miR-10a-3p. Only the liver tissues in the rock bream samples infected by the iridovirus indicated a dominant miR-10a-3p expression. The gene ontology (GO) analysis of predicted target genes for miR-10a-3p revealed that multiple genes are related to binding activity, catalytic activity, cell components as well as cellular and metabolic process. Overall the results imply that the miR-10a-3p could be used as a biomarker to detect VHSV infection in olive flounder and iridovirus infection in rock bream. In addition, the data provides fundamental information for further study of the complex interaction between miR-10a-3p and gene expression. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. Developmental defects in pelagic embryos of several flatfish species in the Southern North sea

    Science.gov (United States)

    Cameron, P.; Berg, J.; Dethlefsen, V.; Von Westernhagen, H.

    In the spring of 1984 through and in the summer of 1987 pelagic flatfish eggs were collected in horizontal subsurface hauls with a 1-m ring net in the southern North Sea between 51°49' to 55°30'N and 2°30' to 8°00'E. The flatfish eggs were observed alive for their developmental stage and aberrations from normal development. Flatfish eggs occurring in the winter ichthyoplankton of the southern North Sea were dab, Limanda limanda; flounder. Platichthys flesus; plaice, Pleuronectes platessa, and long rough dab, Hippoglossoides platessoides. Summer samples contained dab, turbot, Psetta maxima; little sole, Buglossidium luteum; common sole, Solea solea; Norwegian topknot, Phrynorhombus norwegicus, and lemon sole, Microstomus kitt. In winter as well as in summer eggs of dab were the most abundant in the southern North Sea with up to 249 eggs·m -3 surface water in winter and 26 eggs·m -3 in summer. The investigations revealed considerable quantities of abnormally developed flatfish embryos in the plankton samples. Highest malformation rates were recorded for the early stages of dab (44.4%) followed by flounder (41.3%) and plaice (26.0%) in winter; in summer these rates were for dab 18.9%, for turbot 14.1% and for little sole 12.5%. Generally malformation rates decreased with development. Areas of high malformation rates were off the river estuaries and in near-shore areas along the Dutch and German coasts. Malformation rates decreased with the distance from the coast. Causes and effects of the occurrence of malformations during embryonic development are discussed.

  5. Inversión ocular en Paralichthys adspersus (Steindachner, 1867 lenguado de ojos chicos (Pleuronectiformes, Paralichthyidae: un caso del ambiente y un análisis en ejemplares cultivados

    Directory of Open Access Journals (Sweden)

    Héctor Flores

    2013-03-01

    Full Text Available En diversas especies de Pleuronectiformes se han registrado casos de inversión ocular, fenómeno, en que el individuo reposa en el fondo por el lado contrario al habitual y/o normal. La proporción en que se presenta esta condición es variable y depende de las especies que se analicen. En el género Paralichthys se ha reportado inversión ocular en cuatro especies: P. albigutta, P. orbignyanus, P. californicus y P. dentatus. En el presente trabajo, se informa la captura de un ejemplar silvestre de Paralichthys adspersus (Steindachner, 1867, con inversión ocular, capturado en Caldera (Chile. También, se han reportado individuos invertidos de esta especie provenientes de un cultivo, a partir de reproductores que fueron capturados en Bahía Coquimbo. En ejemplares diestros y siniestros proveniente de cultivo, se efectuó comparación de relaciones morfométricas. La proporción de peces con inversión ocular y que provienen de un cultivo, representa 2,2% de la población de peces producidos. Se discute la frecuencia de este tipo de anomalías y las probables causas que las estarían produciendo.

  6. Acute toxicity profile of cadmium revealed by proteomics in brain tissue of Paralichthys olivaceus: Potential role of transferrin in cadmium toxicity

    International Nuclear Information System (INIS)

    Zhu Jinyong; Huang Heqing; Bao Xiaodong; Lin Qingmei; Cai Zongwei

    2006-01-01

    An analytical approach using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) separated proteins from the brain tissue of the fish Paralichthys olivaceus. Approximately 600 protein spots were detected from the brain sample when applying 600 μg protein to a 2D-PAGE gel in the pH range 3.5-10.0. Compared to a control sample, significant changes of 24 protein spots were observed in the fish tissue exposed to acute toxicity of seawater cadmium (SCAT) at 10 ppm for 24 h. Among these spots, nine were down-regulated, nine were up-regulated, two showed high expression, and four showed low expression. The collected spots were identified by peptide mass fingerprinting (PMF) and database search, and they were further classified by LOCtree, a hierarchical system of support vector machines which predict their sub-cellular localization. The amount of transferrin expression in brain cells decreased linearly with the increase of SCAT concentration in seawater. Among the 24 proteins identified on a 2D-PAGE gel, 9 demonstrated a synchronous response to acute cadmium, suggesting that they might represent a biomarker profile. Based on their variable levels and trends on the 2D-PAGE gel this protein (likely to be transferrin) suggesting they might be utilized as biomarkers to investigate cadmium pollution levels in seawater and halobios survival, as well as to evaluate the degree of risk of human fatalities. The results indicate that the application of multiple biomarkers has an advantage over a single biomarker for monitoring levels of environmental contamination

  7. Construction of a subtractive library from hexavalent chromium treated winter flounder (Pseudopleuronectes americanus) reveals alterations in non-selenium glutathione peroxidases

    International Nuclear Information System (INIS)

    Chapman, Laura M.; Roling, Jonathan A.; Bingham, Lacey K.; Herald, Matt R.; Baldwin, William S.

    2004-01-01

    Chromium is released during several industrial processes and has accumulated in some estuarine areas. Its effects on mammals have been widely studied, but relatively little information is available on its effects on fish. Gene expression changes are useful biomarkers that can provide information about toxicant exposure and effects, as well as the health of an organism and its ability to adapt to its surroundings. Therefore, we investigated the effects of Cr(VI) on gene expression in the sediment dwelling fish, winter flounder (Pseudopleuronectes americanus). Winter flounder ranging from 300 to 360 g were injected i.p. with Cr(VI) as chromium oxide at 25 μg/kg chromium in 0.15N KCl. Twenty-four hours following injections, winter flounder were euthanized with MS-222 and the livers were excised. Half of the livers were used to make cytosol and the other half were used to isolate mRNA for subtractive hybridization. Subtractive clones obtained were spotted onto nylon filters, which revealed several genes with potentially altered expression due to Cr(VI), including an α class GST, 1-Cys peroxiredoxin (a non-selenium glutathione peroxidase), a P-450 2X subfamily member, two elongation factors (EF-1 gamma and EF-2), and complement component C3. Semi-quantitative RT-PCR was performed and confirmed that Cr(VI) down-regulated complement component C3, an EST, and two potential glutathione peroxidases, GSTA3 and 1-Cys peroxiredoxin. In addition, cytosolic GSH peroxidase activity was reduced, and silver stained SDS-PAGE gels from glutathione-affinity purified cytosol demonstrated that a 27.1 kDa GSH-binding protein was down-regulated greater than 50%. Taken together, Cr(VI) significantly altered the expression of several genes including two potential glutathione peroxidases in winter flounder

  8. Structural characterization of peptides derived from prosomatostatins I and II isolated from the pancreatic islets of two species of teleostean fish: the daddy sculpin and the flounder.

    Science.gov (United States)

    Conlon, J M; Davis, M S; Falkmer, S; Thim, L

    1987-11-02

    The primary structures of three peptides from extracts from the pancreatic islets of the daddy sculpin (Cottus scorpius) and three analogous peptides from the islets of the flounder (Platichthys flesus), two species of teleostean fish, have been determined by automated Edman degradation. The structures of the flounder peptides were confirmed by fast-atom bombardment mass spectrometry. The peptides show strong homology to residues (49-60), (63-96) and (98-125) of the predicted sequence of preprosomatostatin II from the anglerfish (Lophius americanus). The amino acid sequences of the peptides suggest that, in the sculpin, prosomatostatin II is cleaved at a dibasic amino acid residue processing site (corresponding to Lys61-Arg62 in anglerfish preprosomatostatin II). The resulting fragments are further cleaved at monobasic residue processing sites (corresponding to Arg48 and Arg97 in anglerfish preprosomatostatin II). In the flounder the same dibasic residue processing site is utilised but cleavage at different monobasic sites takes place (corresponding to Arg50 and Arg97 in anglerfish preprosomatostatin II). A peptide identical to mammalian somatostatin-14 was also isolated from the islets of both species and is presumed to represent a cleavage product of prosomatostatin I.

  9. Histopathological effects of chronic aqueous exposure to bis(tri-n-butyltin)oxide (TBTO) to environmentally relevant concentrations reveal thymus atrophy in European flounder (Platichthys flesus)

    Energy Technology Data Exchange (ETDEWEB)

    Grinwis, G.C.M., E-mail: g.c.m.grinwis@uu.n [Department of Pathobiology, Faculty of Veterinary Medicine University of Utrecht, P.O. Box 80.158, 3508 TD Utrecht (Netherlands); Wester, P.W. [Laboratory for Toxicology, Pathology and Genetics, National Institute for Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven (Netherlands); Vethaak, A.D. [Deltares, Unit Coastal and Marine Systems, P.O. Box 177, 2600 MH Delft (Netherlands)

    2009-10-15

    Although the use of tributyltin in antifouling paints has been banned, this compound is still a serious pollutant of the marine environment. This paper describes a unique study in which European flounder (Platichthys flesus) were chronically (8 months) exposed to bis(tri-n-butyltin)oxide (TBTO) in the water under controlled laboratory conditions. Residue levels in selected tissues (liver, muscle) and general health status indices were measured and the effects on several organs (gills, liver, mesonephros, ovary/testis, spleen, and gastrointestinal tract) were examined histopathologically. Additionally, morphometric analysis of the thymus was performed. The major finding is that exposure of flounder to 5 mug TBTO/l over a period of 8 months, resulting in body burdens comparable to high field levels, induced significant reduction of thymus volume, possibly affecting immunocompetence of the animals. Chronic exposure of European flounder to tributyltin is therefore likely to affect the general health status of this species in heavily polluted aquatic environments. - Current levels of tributyltin antifouling contamination are still likely to affect the general health status of estuarine flatfish in heavily polluted environments.

  10. Histopathological effects of chronic aqueous exposure to bis(tri-n-butyltin)oxide (TBTO) to environmentally relevant concentrations reveal thymus atrophy in European flounder (Platichthys flesus)

    International Nuclear Information System (INIS)

    Grinwis, G.C.M.; Wester, P.W.; Vethaak, A.D.

    2009-01-01

    Although the use of tributyltin in antifouling paints has been banned, this compound is still a serious pollutant of the marine environment. This paper describes a unique study in which European flounder (Platichthys flesus) were chronically (8 months) exposed to bis(tri-n-butyltin)oxide (TBTO) in the water under controlled laboratory conditions. Residue levels in selected tissues (liver, muscle) and general health status indices were measured and the effects on several organs (gills, liver, mesonephros, ovary/testis, spleen, and gastrointestinal tract) were examined histopathologically. Additionally, morphometric analysis of the thymus was performed. The major finding is that exposure of flounder to 5 μg TBTO/l over a period of 8 months, resulting in body burdens comparable to high field levels, induced significant reduction of thymus volume, possibly affecting immunocompetence of the animals. Chronic exposure of European flounder to tributyltin is therefore likely to affect the general health status of this species in heavily polluted aquatic environments. - Current levels of tributyltin antifouling contamination are still likely to affect the general health status of estuarine flatfish in heavily polluted environments.

  11. Characterization of an Olive Flounder Bone Gelatin-Zinc Oxide Nanocomposite Film and Evaluation of Its Potential Application in Spinach Packaging.

    Science.gov (United States)

    Beak, Songee; Kim, Hyeri; Song, Kyung Bin

    2017-11-01

    Olive flounder bone gelatin (OBG) was used for a film base material in this study. In addition, zinc oxide nanoparticles (ZnO) were incorporated into the OBG film to prepare a nanocomposite film and to impart antimicrobial activity to it. The tensile strength of the OBG film increased by 6.62 MPa, and water vapor permeability and water solubility decreased by 0.93 × 10 -9 g/m s Pa and 13.79%, respectively, by the addition of ZnO to the OBG film. In particular, the OBG-ZnO film exhibited antimicrobial activity against Listeria monocytogenes. To investigate the applicability of the OBG-ZnO packaging film, fresh spinach was wrapped in this film and stored for a week. The results indicated that the OBG-ZnO film showed antimicrobial activity against L. monocytogenes inoculated on spinach without affecting the quality of spinach, such as vitamin C content and color. Thus, the OBG-ZnO nanocomposite film can be applied as an efficient antimicrobial food packaging material. As a base material of edible films, gelatin was extracted from olive flounder bone, which is fish processing by-product. Olive flounder bone gelatin (OBG) nanocomposite films were prepared with zinc oxide nanoparticles (ZnO). For an application to antimicrobial packaging, spinach was wrapped with the OBG-ZnO nanocomposite film. © 2017 Institute of Food Technologists®.

  12. Profiles of alpha-melanocyte-stimulating hormone in the Japanese flounder as revealed by a newly developed time-resolved fluoroimmunoassay and immunohistochemistry.

    Science.gov (United States)

    Amiya, Noriko; Amano, Masafumi; Takahashi, Akiyoshi; Yamanome, Takeshi; Yamamori, Kunio

    2007-03-01

    Profiles of alpha-melanocyte-stimulating hormone (alpha-MSH) in the Japanese flounder were examined by a newly developed time-resolved fluoroimmunoassay (TR-FIA) and immunohistochemistry. A TR-FIA for alpha-MSH was newly developed, and its levels in the pituitary gland and plasma of Japanese flounder reared in a white or black tank for 5 months were compared. A competitive assay using two antibodies was performed among secondary antibodies in the solid phase, alpha-MSH antibodies, samples, and europium-labeled Des-Ac-alpha-MSH. The sensitivity of the assay, defined as twice the standard deviation at a zero dose, was 0.98 ng/ml (49 pg/well). The intra- and interassay coefficients of variation of the assay were 8.8% (n=8) and 17.3% (n=5), respectively, at about 50% binding. Cross-reactivities of Des-Ac-alpha-MSH and Di-Ac-alpha-MSH were about 100%. Cross-reactivities of adrenocorticotropic hormone, salmon gonadotropin-releasing hormone (sGnRH), and chicken GnRH-II were less than 0.2%, and that of melanin-concentrating hormone was less than 2.0% at 50% binding. Displacement curves of serially twofold-diluted hypothalamus extract, pituitary gland extract, and plasma extract of Japanese flounder with the assay buffer were parallel to the alpha-MSH standard curve. Moreover, displacement curves of serially twofold-diluted hypothalamus and/or pituitary gland extract of masu salmon, goldfish, red seabream, Japanese eel, tiger puffer, and barfin flounder with the assay buffer were also parallel to the alpha-MSH standard. In Japanese flounder, total immunoreactive (ir)-alpha-MSH levels in the pituitary gland were lower in the black tank, whereas those in the plasma tended to be higher in the black tank, suggesting that the synthesis and release of alpha-MSH are higher in the black tank. alpha-MSH-ir cells were detected in the pars intermedia and a small part of the pars distalis of the pituitary gland. alpha-MSH-ir cell bodies were located in the basal hypothalamus and alpha

  13. Comparison of selected biomarkers in flounder (Platichthys flesus L.) from the Douro (Portugal) and Vistula (Poland) River estuaries.

    Science.gov (United States)

    Kopecka-Pilarczyk, Justyna

    2013-08-15

    Sixty female flounder (Platichthys flesus) were collected in Autumn 2011, 15 from each of the following sampling sites: at the mouths of the Douro and Vistula Rivers, and at nearby open sea locations. The aim of the study was to assess several biomarkers in the two geographically distant regions. Hepatic EROD, GST, SOD, GPx, POx, LP; muscular AChE, BChE, LP; and branchial Na(+)/K(+)-ATPase were analysed. Moreover, BTI, PY, and three gross morphometric indices were calculated. The results were analysed with t-test, ANOVA, and PCA. Many differences were found between the open sea sites and the river mouths, mainly in Portugal, and between the two rivers. Salinity and pollution seem to be the main factors that affected the biomarkers. Effects of chronic pollution were observed at the river mouths, and an indication of a possible temporary exposure to pollutants was found at the open ocean site in Portugal. Copyright © 2013 Elsevier Ltd. All rights reserved.

  14. Molecular Characterization and Expression of α-Globin and β-Globin Genes in the Euryhaline Flounder (Platichthys flesus

    Directory of Open Access Journals (Sweden)

    Weiqun Lu

    2011-01-01

    Full Text Available In order to understand the possible role of globin genes in fish salinity adaptation, we report the molecular characterization and expression of all four subunits of haemoglobin, and their response to salinity challenge in flounder. The entire open reading frames of α1-globin and α2-globin genes were 432 and 435 bp long, respectively, whereas the β1-globin and β2-globin genes were both 447 bp. Although the head kidney (pronephros is the predicted major site of haematopoiesis, real-time PCR revealed that expression of α-globin and β-globin in kidney (mesonephros was 1.5 times higher than in head kidney. Notably, the α1-globin and β1-globin mRNA expression was higher than α2-globin and β2-globin in kidney. Expression levels of all four globin subunits were higher in freshwater- (FW- than in seawater- (SW-adapted fish kidney. If globins do play a role in salinity adaptation, this is likely to be more important in combating the hemodilution faced by fish in FW than the dehydration and salt loading which occur in SW.

  15. Environmental and anthropogenic factors affecting the probability of occurrence of Oncomegas wageneri (Cestoda: Trypanorhyncha) in the southern Gulf of Mexico.

    Science.gov (United States)

    Vidal-Martínez, Víctor M; Torres-Irineo, Edgar; Romero, David; Gold-Bouchot, Gerardo; Martínez-Meyer, Enrique; Valdés-Lozano, David; Aguirre-Macedo, M Leopoldina

    2015-11-26

    Understanding the environmental and anthropogenic factors influencing the probability of occurrence of the marine parasitic species is fundamental for determining the circumstances under which they can act as bioindicators of environmental impact. The aim of this study was to determine whether physicochemical variables, polyaromatic hydrocarbons or sewage discharge affect the probability of occurrence of the larval cestode Oncomegas wageneri, which infects the shoal flounder, Syacium gunteri, in the southern Gulf of Mexico. The study area included 162 sampling sites in the southern Gulf of Mexico and covered 288,205 km(2), where the benthic sediments, water and the shoal flounder individuals were collected. We used the boosted generalised additive models (boosted GAM) and the MaxEnt to examine the potential statistical relationships between the environmental variables (nutrients, contaminants and physicochemical variables from the water and sediments) and the probability of the occurrence of this parasite. The models were calibrated using all of the sampling sites (full area) with and without parasite occurrences (n = 162) and a polygon area that included sampling sites with a depth of 1500 m or less (n = 134). Oncomegas wageneri occurred at 29/162 sampling sites. The boosted GAM for the full area and the polygon area accurately predicted the probability of the occurrence of O. wageneri in the study area. By contrast, poor probabilities of occurrence were obtained with the MaxEnt models for the same areas. The variables with the highest frequencies of appearance in the models (proxies for the explained variability) were the polyaromatic hydrocarbons of high molecular weight (PAHH, 95 %), followed by a combination of nutrients, spatial variables and polyaromatic hydrocarbons of low molecular weight (PAHL, 5 %). The contribution of the PAHH to the variability was explained by the fact that these compounds, together with N and P, are carried by rivers that

  16. Southern blotting.

    Science.gov (United States)

    Brown, T

    2001-05-01

    Southern blotting is the transfer of DNA fragments from an electrophoresis gel to a membrane support (the properties and advantages of the different types of membrane, transfer buffer, and transfer method are discussed in detail), resulting in immobilization of the DNA fragments, so the membrane carries a semipermanent reproduction of the banding pattern of the gel. After immobilization, the DNA can be subjected to hybridization analysis, enabling bands with sequence similarity to a labeled probe to be identified. This appendix describes Southern blotting via upward capillary transfer of DNA from an agarose gel onto a nylon or nitrocellulose membrane, using a high-salt transfer buffer to promote binding of DNA to the membrane. With the high-salt buffer, the DNA becomes bound to the membrane during transfer but not permanently immobilized. Immobilization is achieved by UV irradiation (for nylon) or baking (for nitrocellulose). A Support Protocol describes how to calibrate a UV transilluminator for optimal UV irradiation of a nylon membrane. An alternate protocol details transfer using nylon membranes and an alkaline buffer, and is primarily used with positively charged nylon membranes. The advantage of this combination is that no post-transfer immobilization step is required, as the positively charged membrane binds DNA irreversibly under alkaline transfer conditions. The method can also be used with neutral nylon membranes but less DNA will be retained. A second alternate protocol describes a transfer method based on a different transfer-stack setup. The traditional method of upward capillary transfer of DNA from gel to membrane described in the first basic and alternate protocols has certain disadvantages, notably the fact that the gel can become crushed by the weighted filter papers and paper towels that are laid on top of it. This slows down the blotting process and may reduce the amount of DNA that can be transferred. The downward capillary method described in

  17. Disease prevalence in flounder (Platichthys flesus) from the Dutch Wadden Sea as indicator of environmental quality: A summary of 1988-2005 surveys

    Science.gov (United States)

    Vethaak, A. D.

    2013-09-01

    In 1988, epizootics of ulcer disease in the flatfish flounder in the Dutch Wadden Sea were reported near freshwater drainage sluices of IJsselmeer Lake, locally affecting up to 38.9% of fish. Other diseases such as fin rot and lymphocystis were less frequent, but followed a similar pattern. Results of follow-up surveys in the Wadden Sea in 1994-2005 confirm previous findings and also show significantly elevated ulcer prevalences at other smaller drainage works. The most likely stress factors that contributed to the development of the epizootics at these sites include osmotic stress, adverse water quality conditions including chemical contaminants, nutritional deficiencies, and obstruction to fish migration. It was shown that discharges of IJsselmeer Lake freshwater in 1988-96 had a wide effect on the prevalence and distribution of ulcers and lymphocystis in the western Wadden Sea. A general reduction in disease prevalence in flounder in the entire Dutch Wadden Sea was observed during 1988-2005, which was most likely due to a general improvement in water quality and locally improved habitat conditions for flounder near drainage sluices. Ulcer prevalences outside the two IJsselmeer Lake sluices (Den Oever and Kornwerderzand) declined in this period from approximately 30% to 10% for medium-sized fish. Other skin diseases have also displayed a downward trend at both sites in recent years, with prevalences falling sharply to below 1%. Elsewhere in the Wadden Sea and the Ems-Dollard estuary, disease prevalences have declined towards natural background levels (< 1%). It is concluded that skin diseases, especially ulcers, are useful indicators of environmental quality in the Wadden Sea.

  18. Utilization of dietary lipid in young and immature Japanese flounder; Hirame wakagyo, miwseigyo shiryoni okeru shibo no riyono

    Energy Technology Data Exchange (ETDEWEB)

    Kikuchi, K.; Furuta, T.

    2000-02-01

    Effects of dietary protein and lipid levels on growth and body composition of Japanese flounder were examined. Young fish of about 55 g in initial body weight were fed six extruded diets with dietary protein levels of 45 and 55% and three lipid levels each to satiation twice daily. 6 days a week for 12 weeks at 20 degree C with triplicate groups per dietary treatment. Four diets with the same protein level (45 and 55 %) and two lipid levels each were supplied to immature fish of 240 g of initial body weight for 20 weeks with duplicate groups per treatment. The final body weight and weight gain of young fish fed the diets containing 55 %protein were higher than those of fish fed the 45 % protein diets, although both parameters were statistically identical among dietary treatments. The dietary lipid level did not affect these parameters regardless of the protein level. Protein efficiency ratio improved as the lipid level of the diet increased in the 55 % protein diets. The growth of immature fish fed the diet with 55 % protein and the lowest lipid level was higher than that in the other dietary treatment. However, protein efficiency ratio was the highest in the dietary group with 45 % protein and the highest lipid level. Both in two feeding trials, triglyceride content of the cultured fish increased depending on the dietary lipid level, while the other hematological and hematochemical parameters were not affected seriously by the dietary composition. Liver weight and hepato-somatic index of the cultured fish tended to increase in accordance with increasing dietary lipid level both in two trials. Crude lipid content of the li8ver and digestive tract appeared to depend on dietary lipid level. The diet containing 45 % protein and the highest lipid level resulted in significantly higher crude lipid content of the eye-side muscle as well as Engawa muscle of the cultured fish than those in the other dietary groups. (author)

  19. Mechanisms of mercurial and arsenical inhibition of tyrosine absorption in intestine of the winter flounder Pseudopleuronectus americanus

    International Nuclear Information System (INIS)

    Musch, M.W.; Chauncey, B.; Schmid, E.C.; Kinne, R.K.; Goldstein, L.

    1990-01-01

    Effects of HgCl2 (100 microM) para-chloromercuribenzene sulfonate (PCMBS) (1 mM), and oxophenylarsine (OPA) (250 microM) were determined on (a) the rate of Na pump activity in intact winter flounder intestine; (b) activity of Na-K-ATPase in tissue homogenates; and (c) Na-dependent and Na-independent uptake of tyrosine in brush border membrane vesicles. Initial rate of uptake (influx) of 86Rb from the serosal solution of tissues mounted in Ussing chambers, a measure of Na-K-ATPase activity in the intact cell, was inhibited by all three agents with differing time courses. Rapidly permeating HgCl2 inhibited influx to the same degree as ouabain at 30 min, whereas the effects of PCMBS and OPA required 90 min. Cell potassium was also measured as an indirect indicator of ATPase activity and cell membrane permeability. All three agents decreased cell K, although effects on cell K lagged behind those for inhibition of the ATPase. At the concentrations used in the Ussing chamber (or at one-tenth concentration), all agents completely inhibited Na-K-ATPase activity in enzyme assays performed with tissue homogenates. In contrast, only HgCl2 decreased Na-dependent uptake of tyrosine by brush border membrane vesicles. These results suggest that mercurial and arsenical effects on tyrosine absorption are due to inhibition of the Na-K-ATPase thus decreasing the driving force for the cellular uptake by the Na-tyrosine cotransport system. Direct effects on Na-tyrosine cotransport may play a role in the inhibition observed with HgCl2, but not for PCMBS or OPA

  20. Catabolic signaling pathways, atrogenes, and ubiquitinated proteins are regulated by the nutritional status in the muscle of the fine flounder.

    Directory of Open Access Journals (Sweden)

    Eduardo N Fuentes

    Full Text Available A description of the intracellular mechanisms that modulate skeletal muscle atrophy in early vertebrates is still lacking. In this context, we used the fine flounder, a unique and intriguing fish model, which exhibits remarkably slow growth due to low production of muscle-derived IGF-I, a key growth factor that has been widely acknowledged to prevent and revert muscle atrophy. Key components of the atrophy system were examined in this species using a detailed time-course of sampling points, including two contrasting nutritional periods. Under basal conditions high amounts of the atrogenes MuRF-1 and Atrogin-1 were observed. During fasting, the activation of the P38/MAPK and Akt/FoxO signaling pathways decreased; whereas, the activation of the IκBα/NFκB pathway increased. These changes in signal transduction activation were concomitant with a strong increase in MuRF-1, Atrogin-1, and protein ubiquitination. During short-term refeeding, the P38/MAPK and Akt/FoxO signaling pathways were strongly activated, whereas the activation of the IκBα/NFκB pathway decreased significantly. The expression of both atrogenes, as well as the ubiquitination of proteins, dropped significantly during the first hour of refeeding, indicating a strong anti-atrophic condition during the onset of refeeding. During long-term refeeding, Akt remained activated at higher than basal levels until the end of refeeding, and Atrogin-1 expression remained significantly lower during this period. This study shows that the components of the atrophy system in skeletal muscle appeared early in the evolution of vertebrates and some mechanisms have been conserved, whereas others have not. These results represent an important achievement for the area of fish muscle physiology, showing an integrative view of the atrophy system in a non-mammalian species and contributing to novel insights on the molecular basis of muscle growth regulation in earlier vertebrates.

  1. Isolation and characterization of a rhabdovirus from starry flounder (Platichthys stellatus) collected from the northern portion of Puget Sound, Washington, USA

    Science.gov (United States)

    Mork, Christina; Hershberger, Paul K.; Kocan, Richard; Batts, William N.; Winton, James R.

    2004-01-01

    The initial characterization of a rhabdovirus isolated from a single, asymptomatic starry flounder (Platichthys stellatus) collected during a viral survey of marine fishes from the northern portion of Puget Sound, Washington, USA, is reported. Virions were bullet-shaped and approximately 100 nm long and 50 nm wide, contained a lipid envelope, remained stable for at least 14 days at temperatures ranging from -80 to 5 degrees C and grew optimally at 15 degrees C in cultures of epithelioma papulosum cyprini (EPC) cells. The cytopathic effect on EPC cell monolayers was characterized by raised foci containing rounded masses of cells. Pyknotic and dark-staining nuclei that also showed signs of karyorrhexis were observed following haematoxylin and eosin, May-Grunwald Giemsa and acridine orange staining. PAGE of the structural proteins and PCR assays using primers specific for other known fish rhabdoviruses, including Infectious hematopoietic necrosis virus, Viral hemorrhagic septicemia virus, Spring viremia of carp virus, and Hirame rhabdovirus, indicated that the new virus, tentatively termed starry flounder rhabdovirus (SFRV), was previously undescribed in marine fishes from this region. In addition, sequence analysis of 2678 nt of the amino portion of the viral polymerase gene indicated that SFRV was genetically distinct from other members of the family Rhabdoviridae for which sequence data are available. Detection of this virus during a limited viral survey of wild fishes emphasizes the void of knowledge regarding the diversity of viruses that naturally infect marine fish species in the North Pacific Ocean.

  2. Studies on substitutional protein sources for fish meal in the diet of Japanese flounder; Hirame shiryo ni okeru miriyo shigen no riyo

    Energy Technology Data Exchange (ETDEWEB)

    Kikuchi, K; Furuta, T; Sakaguchi, I [Central Research Institute of Electric Power Industry, Tokyo (Japan)

    1996-08-01

    Effectiveness of livestock industry wastes and vegetable protein added to fish meal in fish farming is tested by feeding the Japanese flounder. In the experiment, a part or the whole of the fish meal protein is replaced by the meat meal (MM), meat and bone meal (MBM), corngluten meal (CGM), or dried silkworm pupa meal (SPM), and fries of the Japanese flounder are fed on the new diets for eight weeks. On a diet containing 60% or less of MM, no change is detected in the fish in terms of increase in weight, protein efficiency ratio, and blood components, indicating that 60% at the highest of fish meal may be replaced by MM. In the case of MBM, it can occupy approximately 20%. As for CGM, the proper substitution rate is approximately 40%. Essential amino acids that the new diets may lack are added for an approximately 10% improvement on the result. The SPM substitution works up to 40%, when, however, the blood components are degraded. The proper substitution rate is therefore placed at approximately 20%. 38 refs., 2 figs., 17 tabs.

  3. Relationship between shipping amounts of olive flounder aquacultured from Jejudo and the reported events of acute food poisoning by in 2015, South Korea: an ecological study

    Directory of Open Access Journals (Sweden)

    Jong-Myon Bae

    2017-09-01

    Full Text Available OBJECTIVES Confirmation of Kudoa septempunctata (K. septempunctata as the pathogenic agent causing acute food poisoning remains under debate owing to inconsistencies in the reproducibility of experimental evidence. Higher intake of olive flounder infected with K. septempunctata would result in increased diagnosis of food poisoning by K. septempunctata, if the latter was one of the causal agents of acute food poisoning. The aim was to evaluate the relationship between the shipping amount of olive flounder aquacultured from Jejudo and the incidence of K. septempunctata food poisoning in 2015, Korea. METHODS Data of shipping amounts between March 2014 and February 2016 and of monthly reported events of Kudoa food poisoning were taken from Jejudo Fish-Culture Fisheries Cooperatives and Korea Centers for Disease Control and Prevention, respectively. Non-parametric correlation analyses were conducted. RESULTS Shipping amounts indicated the seasonal changes according to variation of consumption. Spearman’s rho and Kendall’s tau-a between the monthly shipping amounts and the reported events in 2015 were 0.39 (p=0.21 and 0.27 (p=0.20, respectively. CONCLUSIONS An independent relationship was noted between the shipping amount and the reported events, which contrasted with the claim that the virulence of K. septempunctata caused acute food poisoning.

  4. Intraspecific variation in expression of candidate genes for osmoregulation, heme biosynthesis and stress resistance suggests local adaptation in European flounder ( Platichthys flesus )

    DEFF Research Database (Denmark)

    Larsen, Peter Foged; Eg Nielsen, Einar; Williams, T.D.

    2008-01-01

    Despite the recent discovery of significant genetic structuring in a large number of marine organisms, the evolutionary significance of these often minute genetic differences are still poorly understood. To elucidate the adaptive relevance of low genetic differentiation among marine fish...... in osmoregulative processes (Na/K-ATPases-alpha and angiotensinogen) showed highly plastic but similar expression in the two populations dependent on environmental salinity. However, we observed a unique sixfold up-regulation of hsp70 in kidney tissue of flounder from the North Sea following long-term acclimation...... conditions. This identification of adaptive differences in high gene flow marine organisms adds a new dimension to our current understanding of evolutionary processes in the sea and is of paramount importance for identification, protection and sustainable management of marine biodiversity....

  5. Fertilizing Southern Hardwoods

    Science.gov (United States)

    W. M. Broadfoot; A. F. Ike

    1967-01-01

    If present trends continue, fertilizing may soon be economically feasible in southern hardwood stands. Demands for the wood are rising, and the acreage alloted for growing it is steadily shrinking. To supply anticipated requests for information, the U. S. Forest Service has established tree nutrition studies at the Southern Hardwoods Laboratory in Stoneville,...

  6. Southern Gothic Literature

    DEFF Research Database (Denmark)

    Bjerre, Thomas Ærvold

    2017-01-01

    Provides an outline of Southern Gothic Literature, offers an argument about its history and shape, and discusses the scholarly literature surrounding Southern Gothic. Oxford Research Encyclopedia is an online peer-reviewed encyclopedia for researchers, teachers, and students interested in all...... facets of the study of literature...

  7. Southern Identity in "Southern Living" Magazine

    Science.gov (United States)

    Lauder, Tracy

    2012-01-01

    A fantasy-theme analysis of the editors' letters in "Southern Living" magazine shows an editorial vision of valuing the past and showcasing unique regional qualities. In addition, a content analysis of the visual representation of race in the magazine's formative years and recent past validates that inhabitants of the region were portrayed…

  8. European Southern Observatory

    CERN Multimedia

    CERN PhotoLab

    1970-01-01

    Professor A. Blaauw, Director general of the European Southern Observatory, with George Hampton on his right, signs the Agreement covering collaboration with CERN in the construction of the large telescope to be installed at the ESO Observatory in Chile.

  9. University of Southern California

    Data.gov (United States)

    Federal Laboratory Consortium — The focus of the University of Southern California (USC) Children''s Environmental Health Center is to develop a better understanding of how host susceptibility and...

  10. Southern African Business Review

    African Journals Online (AJOL)

    The Southern African Business Review is a refereed and accredited scientific journal of the College of Economic and Management Sciences of the .... The effects of extended water supply disruptions on the operations of SMEs · EMAIL FREE ...

  11. Southern African Business Review

    African Journals Online (AJOL)

    Southern African Business Review. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 20, No 1 (2016) >. Log in or Register to get access to full text downloads.

  12. Reduction of nitrogen in the excretion on Japanese flounder using Ulva and Capitellid; Anaaosa to itogokai ni yoru hirame haisetsubutsuchu no chisso shori

    Energy Technology Data Exchange (ETDEWEB)

    Honda, H.; Kikuchi, K.; Sakaguchi, I. [Central Research Institute of Electric Power Industry, Tokyo (Japan)

    1997-03-01

    To develop the culture residue treatment technique using aquatic organisms, the ammonia and nitrate uptake rates of seaweed Ulva and the nitrogen reduction rate of polychaeta annelid Captella sp. with organic sediment predaceous ability were examined in the excretion of Japanese flounder. Nitrogen uptake rate of Ulva was affected by water temperature. It was highest at 20degC, followed at 15degC and 25degC in the order. It was not affected by light intensity between 1500 and 6000 lux. Ammonia and nitrate uptake rates by Ulva were estimated to be 28.2 and 14.6 {mu}g-N/g/h at 20degC under 3000 lux, respectively. Proportion of feces excreted from Capitellid to ingested sediments was 0.38. At 25degC, Capitellid population of one thousand individuals ingested-N at the rate of 24 mg-N/day, and excreted the feces-N of Capitellid at the rate of 7 mg-N/day. About 70% of nitrogen in the sediment was reduced through this process. 15 refs., 9 figs., 13 tabs.

  13. Southern pulpwood production, 1962

    Science.gov (United States)

    Joe F. Christopher; Martha E. Nelson

    1963-01-01

    Pulpwood production in the south rose to an all-time high of 25,586,300 cords in 1962-58 percent of the Nation's total. At the year's end, 80 southern pulpmills were operating; their combined daily pulping capacity was more than 52,000 tons. Nine mills outside the region were using wood grown in the South.

  14. Multilingualism in Southern Africa.

    Science.gov (United States)

    Peirce, Bonny Norton; Ridge, Stanley G. M.

    1997-01-01

    Reviews recent research in multilingualism in Southern Africa, focusing on the role of languages in education, sociolinguistics, and language policy. Much of the research is on South Africa. Topics discussed include language of instruction in schools, teacher education, higher education, adult literacy, language contact, gender and linguistic…

  15. NREL + Southern California Gas

    Energy Technology Data Exchange (ETDEWEB)

    Berdahl, Sonja E [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2017-10-09

    NREL and Southern California Gas Company are evaluating a new 'power-to-gas' approach - one that produces methane through a biological pathway and uses the expansive natural gas infrastructure to store it. This approach has the potential to change how the power industry approaches renewable generation and energy storage.

  16. Southern Universities Nuclear Institute

    International Nuclear Information System (INIS)

    Anon.

    1977-01-01

    The Southern Universities Nuclear Institute was created in 1961 to provide postgraduate research and teaching facilities for the universities of Cape Town and Stellenbosch. The main research tool is the 6,0 MV Van de Graaff accelerator installed in 1964. Developments and improvements over the years have maintained the Institute's research effectiveness. The work of local research groups has led to a large number of M Sc and doctorate degrees and numerous publications in international journals. Research at the Institute includes front-line studies of basic nuclear and atomic physics, the development and application of nuclear analytical techniques and the application of radioisotope tracers to problems in science, industry and medicine. The Institute receives financial support from the two southern universities, the Department of National Education, the CSIR and the Atomic Energy Board

  17. Southern Alberta system reinforcement

    Energy Technology Data Exchange (ETDEWEB)

    Rehman, A. [Alberta Electric System Operator, Calgary, AB (Canada)

    2008-07-01

    System planning for the Alberta Electric System Operator's (AESO) southern system was discussed in view of the growing interest in developing wind energy resources in the province. While Alberta currently has a total of 11,500 MW of installed wind power, southern Alberta has a very small capability for interconnecting additional wind resources. There are 3 main agencies involved in system planning for the southern region: (1) the Alberta Utilities Commission (AUC), (2) the AESO, and (3) the transmission facility owners. Transmission needs are studied by the AESO, who then applies to the AUC for approval. Transmission facility owners also apply to the AUC for approval to construct facilities. The AESO's roles are to operate the wholesale electricity market; plan the transmission system; arrange access for loads and generation; and oversee transmission system operation. The AESO is an independent agency with a public interest mandate. The AESO's queue management process has been designed to facilitate non-discriminatory system access. Development options currently being considered by the AESO include a 240 kV AC transmission line; a 500 kV AC transmission line; a 765 kV AC transmission line; a high voltage direct current (HVDC) system; and a voltage source converter (VSC) HVDC system. Radial and looped configurations are also being considered. The AESO is currently conducting a participant involvement program that involves open houses with the Canadian Wind Energy Association (CanWEA) and other provincial stakeholders. tabs., figs.

  18. Parasites of the flounder Platichthys flesus (L.) from the German Bight, North Sea, and their potential use in ecosystem monitoring. A. Infection characteristics of potential indicator species

    Science.gov (United States)

    Schmidt, V.; Zander, S.; Körting, W.; Steinhagen, D.

    2003-10-01

    As part of integrated biological-effect monitoring, the parasite fauna of the flounder Platichthys flesus (L.) was investigated at five locations in the German Bight, with a view to using parasite species as bio-indicators. Over a period of 6 years, parasites from 30 different taxa were identified, but only 7 taxa of the parasite community occurred regularly at all locations and in sufficient abundance that they could be considered as potential indicator species. These species were the ciliophoran Trichodina spp., the copepods Acanthochondria cornuta, Lepeophtheirus pectoralis and Lernaeocera branchialis, the helminths Zoogonoides viviparus and Cucullanus heterochrous and metacercaria of an unidentified digenean species. Infection characteristics of these parasites are presented, with a comparison of the results from individual sampling periods and those of the long-term data set. Natural influences on the infection levels, such as temporal variations, habitat conditions and host-related factors, were evaluated. All of these parasite species showed significant differences in their infection levels between the Elbe estuary, as the most polluted site, and the less polluted coastal and marine locations: Helgoland, Outer Eider estuary and Spiekeroog, especially in the long-term data set. Gradual differences between the Elbe, the Outer Eider and Helgoland, which were not detected in individual sampling periods, also became evident in the pooled-data set. These were found in the prevalence of Trichodina spp., A. cornuta, Z. viviparus and C. heterochrous. Although salinity is considered as the most important natural factor, influencing the distribution pattern of the majority of the potential indicator species, infection levels of most of these species differed between locations with similar salinity conditions. Infection levels corresponded to a contamination gradient (Elbe > Inner Eider, Outer Eider > Helgoland) established across the locations. Seasonal variation in

  19. The Southern Ocean Observing System

    OpenAIRE

    Rintoul, Stephen R.; Meredith, Michael P.; Schofield, Oscar; Newman, Louise

    2012-01-01

    The Southern Ocean includes the only latitude band where the ocean circles the earth unobstructed by continental boundaries. This accident of geography has profound consequences for global ocean circulation, biogeochemical cycles, and climate. The Southern Ocean connects the ocean basins and links the shallow and deep limbs of the overturning circulation (Rintoul et al., 2001). The ocean's capacity to moderate the pace of climate change is therefore influenced strongly by the Southern Ocean's...

  20. Southern Pine Beetle Information System (SPBIS)

    Science.gov (United States)

    Valli Peacher

    2011-01-01

    The southern pine beetle (SPB) is the most destructive forest insect in the South. The SPB attacks all species of southern pine, but loblolly and shortleaf are most susceptible. The Southern Pine Beetle Information System (SPBIS) is the computerized database used by the national forests in the Southern Region for tracking individual southern pine beetle infestations....

  1. 77 FR 47373 - New England Fishery Management Council; Public Meeting

    Science.gov (United States)

    2012-08-08

    ..., Southern New England/Mid-Atlantic yellowtail flounder, Georges Bank yellowtail flounder, witch flounder, plaice and Georges Bank/Gulf of Maine white hake. The committee may not develop all the recommendations... accessible to people with disabilities. Requests for sign language interpretation or other auxiliary aids...

  2. Fusiform Rust of Southern Pines

    Science.gov (United States)

    W. R. Phelps; F. L. Czabator

    1978-01-01

    Fusiform rust, caused by the fungus Cronartium fusiforme Hedg. & Hunt ex Cumm., is distributed in the Southern United States from Maryland to Florida and west to Texas and southern Arkansas. Infections by the fungus, which develops at or near the point of infection, result in tapered, spindle-shaped swells, called galls, on branches and stems of pines. (see photo...

  3. Shakespeare in Southern Africa: Submissions

    African Journals Online (AJOL)

    Shakespeare in Southern Africa publishes articles, commentary and reviews on all aspects of Shakespearean studies and performance, with a particular emphasis on responses to Shakespeare in southern Africa. Submissions are reviewed by at least two referees. The practice of 'blind' reviewing is adhered to. The Journal ...

  4. Tornado Strikes Southern Maryland

    Science.gov (United States)

    2002-01-01

    Evening light catches the tops of towering thunderheads over the Mid-Atlantic states on April 28, 2002. The powerful storms spawned several tornados, one of which was classified as an F4 tornado. The powerful tornado touched down in the southern Maryland town of La Plata, destroying most of the historic downtown. The twister-one of the strongest ever to hit the state-beat a 24-mile swath running west to east through the state and claimed at least three lives. The image above was taken by the Geostationary Operational Environmental Satellite (GOES) at 7:15 PM Eastern Daylight Savings Time. A large version of the animation shows more detail. (5.9 MB Quicktime) Image courtesy National Oceanic and Atmospheric Administration (NOAA) and the GOES Project Science Office. Animation by Robert Simmon, NASA GSFC.

  5. The Mysterious Southern Torque

    Science.gov (United States)

    McDowell, M. S.

    2004-05-01

    Something weird happened to twist the southern hemisphere out of alignment with the northern, as evidenced by the positions of the mountain ranges of North and South America, the Atlantic MAR, and the closure of West Africa to North America - all smooth were the torque reversed. What happened, and when, and why? We identify a number of global "cracks" of almost exactly the same length and direction, with some, even more peculiarly, turning the same angle, and proceeding an equal distance in the new direction. The Emperor-Hawaiian chain, the Louisville chain and the west coast of North America, as examples, are essentially parallel. Their northerly legs follow the angle of the axis of orbital ellipse. But then they all make equal 45 degree easterly bends, to 17.5 NW, and continue on, still parallel, for very similar distances. It is the same at the north coast of South America, and the mid-section of the MAR from 46W to 12W. It is the distance from the Cameroons to Kenya, from the south end of the Red Sea to the SE Indian Ridge at the Nema Fracture zone, from west to east of the Nazca plate.What is all this? Coincidence? Seeing things? Researchers have attributed plate motion or hot spot motion or both or absolutely none, to all of the above. Geophysicists have dated the surfaces from Archean to Pleistocene by all possible scientific means, certainly no possible correlation can be made. Yet we postulate the physical reality can be demonstrated. It is so global a phenomenon that it is well beyond what a hot spot or a plate could do. Even a really tremendous impact would have trouble making such precise geometric arrangements. So what is it - perhaps the angle of rotation, or the inertia of northern hemisphere mass above the geoid? And if so, then, what changed it? It would seem that some huge imbalance occurred. Suppose the whole bottom blew out of the southern hemisphere, and the center of mass drastically altered. Suppose some unknown universal force changed our

  6. Methodological issues affecting the study of fish parasites. III. Effect of fish preservation method

    Czech Academy of Sciences Publication Activity Database

    Kvach, Yuriy; Ondračková, Markéta; Janáč, Michal; Jurajda, Pavel

    2018-01-01

    Roč. 127, č. 3 (2018), s. 213-224 ISSN 0177-5103 R&D Projects: GA ČR(CZ) GBP505/12/G112 Institutional support: RVO:68081766 Keywords : flounder Paralichthys-olivaceus * Neoheterobothrium-hirame * community structure * infection levels * Baltic sea * Odontobutidae * ectoparasites * Perciformes * collection * ecology * Parasite community * Preservation methods * Perca fluviatilis * Rhodeus amarus * Methodology * Parasitological examination Subject RIV: GL - Fish ing OBOR OECD: Fish ery Impact factor: 1.549, year: 2016

  7. THE SOUTHERN AEGEAN SYSTEM

    Directory of Open Access Journals (Sweden)

    Ina Berg

    2015-08-01

    Full Text Available Although world-systems theory was originally formulated with our modern economic system in mind (Wallerstein 1974, it was not long before archaeologists began to apply it to ancient societies. Archaeologists and world-system theorists alike both argued that Wallerstein had disregarded evidence of interconnected, hierarchical systems in prehistoric times (Schneider 1977; Chase-Dunn & Hall 1991, 1997; Kardulias 1999a. Pailes and Whitecotton (1979 were among the first to modify world-systems theory for use in pre-capitalist settings. Since then many archaeologists have looked at data and regions with a world-systems perspective in mind (e.g. Champion 1989; Bilde et al. 1993; Rowlands & Larsen 1987; Kardulias 1999a. Some have attempted to map Wallerstein's theory directly onto prehistory (Kohl 1979; Whitecotton & Pailes 1986; Ekholm & Friedman 1982. Others have found the world systems model heuristically useful but lacking the analytical power needed for their prehistoric cases (Blanton et al. 1981; Upham 1982; Plog 1983; Alcock 1993. Building on the assumption that ancient societies were not qualitatively, but only quantitatively, different from modern capitalist ones (Schneider 1977; Sherratt & Sherratt 1991, this study applies world systems theory to the Southern Aegean during the Middle and Late Bronze Age (ca. 2000-1550 BC.

  8. Invertebrate diversity in southern California

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This shapefile displays mean invertebrate diversity within 5 minute grid cells. The Shannon Index of diversity was calculated from Southern California Coastal Water...

  9. Southern African Business Review: Submissions

    African Journals Online (AJOL)

    The Southern African Business Review is a refereed and accredited journal of the College of Economic and Management Sciences of the University of South Africa. ... the right to make minor editorial adjustments without consulting the author.

  10. Utilization of the southern pines

    Energy Technology Data Exchange (ETDEWEB)

    Koch, P

    1972-01-01

    After several years out of print, this book is again available. The two-volume reference characterizes the southern pine tree as raw material and describes the process by which it is converted to use. All 10 species are considered. The book is addressed primarily to the incoming generation of researchers and industrial managers in the southern pine industry. Foremen, superintendents, quality control personnel, wood procurement men, forest managers, extension workers, professors, and students of wood technology should find the handbook of value.

  11. Energy Trade in Southern Africa

    International Nuclear Information System (INIS)

    Holland, W F.

    1996-01-01

    This document deals with possible energy growth in Southern African countries. This region possesses substantial energy resources (including fossil fuels), but because of political instability, government intervention, financial paralysis and lack of adequate transportation infrastructure, this region faces problems in satisfying energy needs. Two key international actions, namely the South African Development Community (SADC) Energy Protocol and the Southern African Power Pool (SAPP) are expected to enhance energy trade and promote economic development. (TEC)

  12. Treasures of the Southern Sky

    CERN Document Server

    Gendler, Robert; Malin, David

    2011-01-01

    In these pages, the reader can follow the engaging saga of astronomical exploration in the southern hemisphere, in a modern merger of aesthetics, science, and a story of human endeavor. This book is truly a celebration of southern skies.  Jerry Bonnell, Editor - Astronomy Picture of the Day The southern sky became accessible to scientific scrutiny only a few centuries ago, after the first European explorers ventured south of the equator. Modern observing and imaging techniques have since revealed what seems like a new Universe, previously hidden below the horizon, a fresh astronomical bounty of beauty and knowledge uniquely different from the northern sky. The authors have crafted a book that brings this hidden Universe to all, regardless of location or latitude. Treasures of the Southern Sky celebrates the remarkable beauty and richness of the southern sky in words and with world-class imagery. In part, a photographic anthology of deep sky wonders south of the celestial equator, this book also celebrates th...

  13. REC profile 2: Southern Electric

    International Nuclear Information System (INIS)

    Anon.

    1995-01-01

    A review is presented of South Electric plc, one of the United Kingdom's Regional Electricity Companies (RECs), at present distributing electric power in central southern England. Known in financial markets as an efficient performer, the staff reductions and changes of employment conditions undertaken to achieve this end are described. The need to maintain success in its core business, while simultaneously seeking diversification, drive the company's keen competitive edge. The relationship between the RECs and the electricity regulator is also explored, as Southern Electric considers a friendly merger with another REC to improve its market competitiveness in price terms. (UK)

  14. Southern states' routing agency report

    International Nuclear Information System (INIS)

    1989-02-01

    The Southern states' routing agency report is a compendium of 16-southern states' routing programs relative to the transportation of high-level radioactive materials. The report identifies the state-designated routing agencies as defined under 49 Code of Federal Regulations (CFR) Part 171 and provides a reference to the source ad scope of the agencies' rulemaking authority. Additionally, the state agency and contact designated by the state's governor to receive advance notification and shipment routing information under 10 CFR Parts 71 and 73 are also listed

  15. Southern States' Routing Agency Report

    International Nuclear Information System (INIS)

    1990-03-01

    The Southern States' Routing Agency Report is a compendium of 16-southern states' routing program for the transportation of high-level radioactive materials. The report identifies the state-designated routing agencies as defined under 49 Code of Federal Regulations (CFR) Part 171 and provides a reference to the source and scope of the agencies' rulemaking authority. Additionally, the state agency and contact designated by the state's governor to receive advance notification and shipment routing information under 10 CFR Parts 71 and 73 are also listed

  16. Sexty Southerners: Sexting Content and Behaviors among Selected Southern Undergraduates

    Science.gov (United States)

    Hudson, Heather K.; Marshall, S. Alexandra

    2016-01-01

    Sexting is defined as sending/posting/sharing sexually explicit messages or nude/semi-nude images via electronic communication. Purpose: The purpose of this study was to assess and determine relationships of sexting behavioral intentions, sexting behaviors, and sexting content among selected Southern undergraduate students. Methods: Survey…

  17. Southern African Journal of Environmental Education

    African Journals Online (AJOL)

    The Southern African Journal of Environmental Education (SAJEE) is an accredited and ... It is published at least once a year, by the Environmental Education Association of Southern Africa (EEASA). ... AJOL African Journals Online. HOW TO ...

  18. Southern Coal Corporation Clean Water Settlement

    Science.gov (United States)

    Southern Coal Corporation is a coal mining and processing company headquartered in Roanoke, VA. Southern Coal Corporation and the following 26 affiliated entities are located in Alabama, Kentucky, Tennessee, Virginia and West Virginia

  19. Review of Southern African Studies: Journal Sponsorship

    African Journals Online (AJOL)

    Review of Southern African Studies: Journal Sponsorship. Journal Home > About the Journal > Review of Southern African Studies: Journal Sponsorship. Log in or Register to get access to full text downloads.

  20. Review of Southern African Studies

    African Journals Online (AJOL)

    Review of Southern African Studies is a multidisciplinary journal of Arts, Social and Behavioural Sciences. Vol 13, No 1 (2009). DOWNLOAD FULL TEXT Open Access DOWNLOAD FULL TEXT Subscription or Fee Access. Table of Contents. Articles. Health-Care Waste Practices in Selected Health-Care Facilities in Maseru ...

  1. Unprecedented emergency in Southern Africa.

    Science.gov (United States)

    1999-03-01

    Despite knowledge of better prevention strategies, AIDS continues to be an unprecedented emergency in southern Africa. Statistics show that in 1998, 1.4 million people between the ages of 15 and 49 in the 9 countries of southern Africa were infected, with nearly three-quarters of a million of these new infections occurring in South Africa. In addition, some 2 million people died of AIDS in sub-Saharan Africa in 1998 and millions of new infections are occurring every year. Factors such as the loneliness suffered by migrant laborers, the wars and armed conflicts in Rwanda, and the stigma of shame, silence, and denial associated with AIDS all generate fertile conditions for the spread of HIV in southern Africa. Overcoming silence and denial, and bringing AIDS out into the open, has been considered by some countries in southern Africa. In Botswana and South Africa, appeals for greater awareness and openness by the top leadership have been coupled with a decision to set up government funding and AIDS. The challenge now will be to translate these into effective prevention and care programs.

  2. Pteropods in Southern Ocean ecosystems

    Science.gov (United States)

    Hunt, B. P. V.; Pakhomov, E. A.; Hosie, G. W.; Siegel, V.; Ward, P.; Bernard, K.

    2008-09-01

    To date, little research has been carried out on pelagic gastropod molluscs (pteropods) in Southern Ocean ecosystems. However, recent predictions are that, due to acidification resulting from a business as usual approach to CO 2 emissions (IS92a), Southern Ocean surface waters may begin to become uninhabitable for aragonite shelled thecosome pteropods by 2050. To gain insight into the potential impact that this would have on Southern Ocean ecosystems, we have here synthesized available data on pteropod distributions and densities, assessed current knowledge of pteropod ecology, and highlighted knowledge gaps and directions for future research on this zooplankton group. Six species of pteropod are typical of the Southern Ocean south of the Sub-Tropical Convergence, including the four Thecosomes Limacina helicina antarctica, Limacina retroversa australis, Clio pyramidata, and Clio piatkowskii, and two Gymnosomes Clione limacina antarctica and Spongiobranchaea australis. Limacina retroversa australis dominated pteropod densities north of the Polar Front (PF), averaging 60 ind m -3 (max = 800 ind m -3) and 11% of total zooplankton at the Prince Edward Islands. South of the PF L. helicina antarctica predominated, averaging 165 ind m -3 (max = 2681 ind m -3) and up to >35% of total zooplankton at South Georgia, and up to 1397 ind m -3 and 63% of total zooplankton in the Ross Sea. Combined pteropods contributed 40% of community grazing impact. Further research is required to quantify diet selectivity, the effect of phytoplankton composition on growth and reproductive success, and the role of carnivory in thecosomes. Life histories are a significant knowledge gap for Southern Ocean pteropods, a single study having been completed for L. retroversa australis, making population studies a priority for this group. Pteropods appear to be important in biogeochemical cycling, thecosome shells contributing >50% to carbonate flux in the deep ocean south of the PF. Pteropods may also

  3. Thunderstorms caused by southern cyclones in Estonia

    OpenAIRE

    Kaupo Mändla; Sven-Erik Enno; Mait Sepp

    2014-01-01

    The relationships between the frequency and duration of thunderstorms, lightning and southern cyclones over Estonia are presented for the period 1950–2010. A total of 545 southern cyclones and 2106 thunderstorm days were detected, whereas 11.3% of the observed thunder days were associated with southern cyclones. At the same time, 29.2% of all southern cyclones were accompanied by thunderstorms. In the thunder season, however, this percentage was much higher, reaching up to 80% in summer month...

  4. The Southern Ocean biogeochemical divide.

    Science.gov (United States)

    Marinov, I; Gnanadesikan, A; Toggweiler, J R; Sarmiento, J L

    2006-06-22

    Modelling studies have demonstrated that the nutrient and carbon cycles in the Southern Ocean play a central role in setting the air-sea balance of CO(2) and global biological production. Box model studies first pointed out that an increase in nutrient utilization in the high latitudes results in a strong decrease in the atmospheric carbon dioxide partial pressure (pCO2). This early research led to two important ideas: high latitude regions are more important in determining atmospheric pCO2 than low latitudes, despite their much smaller area, and nutrient utilization and atmospheric pCO2 are tightly linked. Subsequent general circulation model simulations show that the Southern Ocean is the most important high latitude region in controlling pre-industrial atmospheric CO(2) because it serves as a lid to a larger volume of the deep ocean. Other studies point out the crucial role of the Southern Ocean in the uptake and storage of anthropogenic carbon dioxide and in controlling global biological production. Here we probe the system to determine whether certain regions of the Southern Ocean are more critical than others for air-sea CO(2) balance and the biological export production, by increasing surface nutrient drawdown in an ocean general circulation model. We demonstrate that atmospheric CO(2) and global biological export production are controlled by different regions of the Southern Ocean. The air-sea balance of carbon dioxide is controlled mainly by the biological pump and circulation in the Antarctic deep-water formation region, whereas global export production is controlled mainly by the biological pump and circulation in the Subantarctic intermediate and mode water formation region. The existence of this biogeochemical divide separating the Antarctic from the Subantarctic suggests that it may be possible for climate change or human intervention to modify one of these without greatly altering the other.

  5. Photographs of the southern heavens

    CERN Document Server

    West, R M

    1975-01-01

    The 1 m Schmidt telescope of the European Southern Observatory (ESO) has been used for a number of sky surveys. In particular a main task has been an examination of the southern night sky between declinations of -20 degrees and -90 degrees . This exercise is known as the ESO(B) Survey (or Quick Blue Survey); some of the more interesting results are shown and are briefly discussed. The photographic plates used were Kodak II a-o. There are photographs of the two Magellanic Clouds and of the galaxy NGC 1313 and the globular cluster NGC 6752. A spectrogram of our Galaxy for wavelengths in the band 3900 A to 4900 A, taken with the telescope's large objective prism is also shown. (0 refs).

  6. Identification of southern radio sources

    International Nuclear Information System (INIS)

    Savage, A.; Bolton, J.G.; Wright, A.E.

    1976-01-01

    Identifications are suggested for 36 radio sources from the southern zones of the Parkes 2700 MHz survey, 28 with galaxies, six with confirmed and two with suggested quasi-stellar objects. The identifications were made from the ESO quick blue survey plates, the SRC IIIa-J deep survey plates and the Palomar sky survey prints. Accurate optical positions have also been measured for nine of the objects and for five previously suggested identifications. (author)

  7. Southern Great Plains Safety Orientation

    Energy Technology Data Exchange (ETDEWEB)

    Schatz, John

    2014-05-01

    Welcome to the Atmospheric Radiation Measurement (ARM) Climate Research Facility (ARM) Southern Great Plains (SGP) site. This U.S. Department of Energy (DOE) site is managed by Argonne National Laboratory (ANL). It is very important that all visitors comply with all DOE and ANL safety requirements, as well as those of the Occupational Safety and Health Administration (OSHA), the National Fire Protection Association, and the U.S. Environmental Protection Agency, and with other requirements as applicable.

  8. Southern Appalachian Regional Seismic Network

    Energy Technology Data Exchange (ETDEWEB)

    Chiu, S.C.C.; Johnston, A.C.; Chiu, J.M. [Memphis State Univ., TN (United States). Center for Earthquake Research and Information

    1994-08-01

    The seismic activity in the southern Appalachian area was monitored by the Southern Appalachian Regional Seismic Network (SARSN) since late 1979 by the Center for Earthquake Research and Information (CERI) at Memphis State University. This network provides good spatial coverage for earthquake locations especially in east Tennessee. The level of activity concentrates more heavily in the Valley and Ridge province of eastern Tennessee, as opposed to the Blue Ridge or Inner Piedmont. The large majority of these events lie between New York - Alabama lineament and the Clingman/Ocoee lineament, magnetic anomalies produced by deep-seated basement structures. Therefore SARSN, even with its wide station spacing, has been able to define the essential first-order seismological characteristics of the Southern Appalachian seismic zone. The focal depths of the southeastern U.S. earthquakes concentrate between 8 and 16 km, occurring principally beneath the Appalachian overthrust. In cross-sectional views, the average seismicity is shallower to the east beneath the Blue Ridge and Piedmont provinces and deeper to the west beneath the Valley and Ridge and the North American craton. Results of recent focal mechanism studies by using the CERI digital earthquake catalog between October, 1986 and December, 1991, indicate that the basement of the Valley and Ridge province is under a horizontal, NE-SW compressive stress. Right-lateral strike-slip faulting on nearly north-south fault planes is preferred because it agrees with the trend of the regional magnetic anomaly pattern.

  9. Southern Appalachian Regional Seismic Network

    International Nuclear Information System (INIS)

    Chiu, S.C.C.; Johnston, A.C.; Chiu, J.M.

    1994-08-01

    The seismic activity in the southern Appalachian area was monitored by the Southern Appalachian Regional Seismic Network (SARSN) since late 1979 by the Center for Earthquake Research and Information (CERI) at Memphis State University. This network provides good spatial coverage for earthquake locations especially in east Tennessee. The level of activity concentrates more heavily in the Valley and Ridge province of eastern Tennessee, as opposed to the Blue Ridge or Inner Piedmont. The large majority of these events lie between New York - Alabama lineament and the Clingman/Ocoee lineament, magnetic anomalies produced by deep-seated basement structures. Therefore SARSN, even with its wide station spacing, has been able to define the essential first-order seismological characteristics of the Southern Appalachian seismic zone. The focal depths of the southeastern U.S. earthquakes concentrate between 8 and 16 km, occurring principally beneath the Appalachian overthrust. In cross-sectional views, the average seismicity is shallower to the east beneath the Blue Ridge and Piedmont provinces and deeper to the west beneath the Valley and Ridge and the North American craton. Results of recent focal mechanism studies by using the CERI digital earthquake catalog between October, 1986 and December, 1991, indicate that the basement of the Valley and Ridge province is under a horizontal, NE-SW compressive stress. Right-lateral strike-slip faulting on nearly north-south fault planes is preferred because it agrees with the trend of the regional magnetic anomaly pattern

  10. Agro-fuels: southern impacts

    International Nuclear Information System (INIS)

    Duterme, B.

    2011-01-01

    At a time of controversies about global warming, agro-fuels production is often presented as a future solution for automotive fuels and as a new way of development for southern countries. However, in Asia, South America and Africa, it very often takes the form of huge mono-culture areas of sugar cane, palm oil and soy bean in the hands of multinational companies of the agriculture and food industry. The consequences of these land, social and environmental impacts in Southern countries is an increase of disequilibria and a threat for the diet of local populations by changing the appropriation of lands. Deforestation, private monopolization of lands, concentration of profits, soils and water pollution, biodiversity impoverishment, exploitation of vulnerable manpower, populations migration, violation of human rights are today the consequences of mass production of agro-fuels in Southern countries. To what conditions a fair re-appropriation and a sustainable development of agro-fuels production and consumption is foreseeable? The solutions require a re-shaping of economical and agricultural politics. The book gathers testimonies and analyses from specialists who have evaluated locally the consequences of these energy choices. (J.S.)

  11. Atmospheric Chemistry Over Southern Africa

    Science.gov (United States)

    Gatebe, Charles K.; Levy, Robert C.; Thompson, Anne M.

    2011-01-01

    During the southern African dry season, regional haze from mixed industrial pollution, biomass burning aerosol and gases from domestic and grassland fires, and biogenic sources from plants and soils is worsened by a semi-permanent atmosphere gyre over the subcontinent. These factors were a driver of several major international field campaigns in the 1990s and early 2000s, and attracted many scientists to the region. Some researchers were interested in understanding fundamental processes governing chemistry of the atmosphere and interaction with climate change. Others found favorable conditions for evaluating satellite-derived measurements of atmospheric properties and a changing land surface. With that background in mind a workshop on atmospheric chemistry was held in South Africa. Sponsored by the International Commission for Atmospheric Chemistry and Global Pollution (ICACGP; http://www.icacgp.org/), the workshop received generous support from the South African power utility, Eskom, and the Climatology Research Group of the University of the Witwatersrand, Johannesburg, South Africa. The purpose of the workshop was to review some earlier findings as well as more recent findings on southern African climate vulnerability, chemical changes due to urbanization, land-use modification, and how these factors interact. Originally proposed by John Burrows, president of ICACGP, the workshop was the first ICACGP regional workshop to study the interaction of air pollution with global chemical and climate change. Organized locally by the University of the Witwatersrand, the workshop attracted more than 60 delegates from South Africa, Mozambique, Botswana, Zimbabwe, France, Germany, Canada, and the United States. More than 30 presentations were given, exploring both retrospective and prospective aspects of the science. In several talks, attention was focused on southern African chemistry, atmospheric pollution monitoring, and climate processes as they were studied in the field

  12. Thunderstorms caused by southern cyclones in Estonia

    Directory of Open Access Journals (Sweden)

    Kaupo Mändla

    2014-05-01

    Full Text Available The relationships between the frequency and duration of thunderstorms, lightning and southern cyclones over Estonia are presented for the period 1950–2010. A total of 545 southern cyclones and 2106 thunderstorm days were detected, whereas 11.3% of the observed thunder days were associated with southern cyclones. At the same time, 29.2% of all southern cyclones were accompanied by thunderstorms. In the thunder season, however, this percentage was much higher, reaching up to 80% in summer months. The number of thunder days was largest when the centres of southern cyclones passed a measuring station at a distance less than 500 km. The number of cloud-to-ground lightning strikes related to southern cyclones was larger than that of any other thunder events. The results of our study demonstrate that the intensity of thunderstorms related to southern cyclones is higher than that of other thunderstorms. Correlation analysis revealed statistically significant relationships between the frequency of thunder days related to southern cyclones and the frequency of southern cyclones, also between the frequency of thunder days related to southern cyclones and days of other thunder events.

  13. Subduction in the Southern Caribbean

    Science.gov (United States)

    Levander, A.; Schmitz, M.; Bezada, M.; Masy, J.; Niu, F.; Pindell, J.

    2012-04-01

    The southern Caribbean is bounded at either end by subduction zones: In the east at the Lesser Antilles subduction zone the Atlantic part of the South American plate subducts beneath the Caribbean. In the north and west under the Southern Caribbean Deformed Belt accretionary prism, the Caribbean subducts under South America. In a manner of speaking, the two plates subduct beneath each other. Finite-frequency teleseismic P-wave tomography confirms this, imaging the Atlantic and the Caribbean subducting steeply in opposite directions to transition zone depths under northern South America (Bezada et al, 2010). The two subduction zones are connected by the El Pilar-San Sebastian strike-slip fault system, a San Andreas scale system. A variety of seismic probes identify where the two plates tear as they begin to subduct (Niu et al, 2007; Clark et al., 2008; Miller et al. 2009; Masy et al, 2009). The El Pilar system forms at the southeastern corner of the Antilles subduction zone by the Atlantic tearing from South America. The deforming plate edges control mountain building and basin formation at the eastern end of the strike-slip system. In northwestern South America the Caribbean plate tears, its southernmost element subducting at shallow angles under northernmost Colombia and then rapidly descending to transition zone depths under Lake Maracaibo (Bezada et al., 2010). We believe that the flat slab produces the Merida Andes, the Perija, and the Santa Marta ranges. The southern edge of the nonsubducting Caribbean plate underthrusts northern Venezuela to about the width of the coastal mountains (Miller et al., 2009). We infer that the underthrust Caribbean plate supports the coastal mountains, and controls continuing deformation.

  14. Identification of southern radio sources

    International Nuclear Information System (INIS)

    Savage, A.; Bolton, J.G.; Wright, A.E.

    1977-01-01

    Identifications are suggested for 53 radio sources from the southern zones of the Parkes 2700-MHz survey, 32 with galaxies, 11 with suggested QSOs and 10 with confirmed QSOs. The identifications were made from the ESO quick blue survey plates, the SRC IIIa-J deep survey plates and the Palomar Sky Survey prints. Accurate optical positions have been measured for four of the new identifications and for two previously suggested identifications. A further nine previously suggested QSO identifications have also been confirmed by two-colour photography or spectroscopy. (author)

  15. Rural migration in southern Nevada

    International Nuclear Information System (INIS)

    Mosser, D.; Soden, D.L.

    1993-01-01

    This study reviews the history of migration in two rural counties in Southern Nevada. It is part of a larger study about the impact of a proposed high-level nuclear waste repository on, in and out-migration patterns in the state. The historical record suggests a boom and bust economic cycle has predominated in the region for the past century creating conditions that should be taken into account, by decision makers, when ascertaining the long-term impacts of the proposed repository

  16. TTVs study in southern stars

    Science.gov (United States)

    Petrucci, Romina; Jofré, Emiliano; Schwartz, Martín; Buccino, Andrea; Mauas, Pablo

    2012-07-01

    In this contribution we present 4 complete planetary transits observed with the 40-cm telescope ``Horacio Ghielmetti'' located in San Juan(Argentina). These objects correspond to a continuous photometric monitoring program of Southern planet host-stars that we are carrying out since mid-2011. The goal of this project is to detect additional planetary mass objects around stars with known transiting-planets through Transit Timing Variations (TTVs). For all 4 transits the depth and duration are in good agreement with the values published in the discovery papers.

  17. Identification of southern radio sources

    International Nuclear Information System (INIS)

    Savage, A.

    1976-01-01

    Identifications are suggested for 32 radio sources from the southern zones of the Parkes 2700 MHz survey, 18 with galaxies, one with a confirmed and 12 with possible quasistellar objects, and one with a supernova remnant in the Large Magellanic Cloud. The identifications were made from the ESO IIa-O quick blue survey plates, the SRC IIIa-J deep survey plates and the Palomar sky survey prints. Accurate optical positions have also been measured for 10 of the objects and for five previously suggested QSOs. (author)

  18. Southern high-velocity stars

    International Nuclear Information System (INIS)

    Augensen, H.J.; Buscombe, W.

    1978-01-01

    Using the model of the Galaxy presented by Eggen, Lynden-Bell and Sandage (1962), plane galactic orbits have been calculated for 800 southern high-velocity stars which possess parallax, proper motion, and radial velocity data. The stars with trigonometric parallaxes were selected from Buscombe and Morris (1958), supplemented by more recent spectroscopic data. Photometric parallaxes from infrared color indices were used for bright red giants studied by Eggen (1970), and for red dwarfs for which Rodgers and Eggen (1974) determined radial velocities. A color-color diagram based on published values of (U-B) and (B-V) for most of these stars is shown. (Auth.)

  19. Preparing Soil for Turfgrass Establishment - Southern Utah

    OpenAIRE

    Caron, Michael; Schaible, Candace; Heflebower, Rick; Cardon, Grant; Beddes, Taun; Kopp, Kelly

    2017-01-01

    This fact sheet provides information for successfully establishing a lawn including planning, soil preparation, appropriate seed or sod choice, and an understanding of turfgrass requirements in southern Utah.

  20. Future climate change over Southern Africa

    CSIR Research Space (South Africa)

    Davis, Claire L

    2017-10-01

    Full Text Available This chapter presents key messages drawn from recent subsets of future climate projections for the southern Africa region. Material in this chapter is drawn from Chapter 3 of Climate Risk and Vulnerability: A Handbook for Southern Africa (Davis 2011...

  1. The Southern Forest Futures Project: technical report

    Science.gov (United States)

    David N. Wear; John G. Greis

    2013-01-01

    Please visit the Southern Forest Futures Project website for more information.The Southern Forest Futures Project provides a science-based “futuring” analysis of the forests of the 13 States of the Southeastern United States. With findings...

  2. THE ORIGINS OF THE SOUTHERN AFRICAN MAMMAL FAUNA ...

    African Journals Online (AJOL)

    Southern Africa can be geographically subdivided into different biotic zones, differing from .... The greater part of the southern African mammal fauna consists of savanna .... spread into southern Africa by way of the Savanna biotic zone.

  3. Power generation in Southern Africa

    International Nuclear Information System (INIS)

    Beer, J.A. de

    2002-01-01

    This paper outlines the main characteristics of power generation in Southern Africa, in terms of primary energy resources, existing and projected power supply and demand, types and location of power plants, regional integration, and environmental management aspects. Various options for future development of power generation are presented as part of an overall integrated resource planning (IRP) process for the power industry. These include coal and natural gas based options, hydro power and other renewable energy, and nuclear power plants. A specific option, the pebble bed modular reactor (PBMR), under development by Eskom Enterprises and other international and local partners, is described in terms of overall design parameters, inherent safety features, economics and environmental aspects. Included is a high level discussion on the selection of materials for the design of this PBMR plant, an advanced design version of a high temperature gas reactor (HTGR). (orig.)

  4. Southern Energy Efficiency Center (SEEC)

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Robin; Sonne, Jeffrey; Withers, Charles; Cummings, James; Verdict, Malcolm; Roberts, Sydney

    2009-09-30

    The Southern Energy Efficiency Center (SEEC) builds collaborative partnerships with: state and local governments and their program support offices, the building delivery industry (designers, contractors, realtors and commissioning agents), product manufacturers and their supply chains, utilities and their program implementers, consumers and other stakeholders in order to forge a strong regional network of building energy efficiency allies. Through a project Steering Committee composed of the state energy offices and building industry stakeholders, the SEEC works to establish consensus-based goals, priorities and strategies at the regional, state and local levels that will materially advance the deployment of high-performance “beyond code” buildings. In its first Phase, SEEC will provide limited technical and policy support assistance, training, certification and education to a wide spectrum of the building construction, codes and standards, and the consumer marketplace.

  5. Discussing epigenetics in Southern California

    Science.gov (United States)

    2012-01-01

    With the goal of discussing how epigenetic control and chromatin remodeling contribute to the various processes that lead to cellular plasticity and disease, this symposium marks the collaboration between the Institut National de la Santé et de la Recherche Médicale (INSERM) in France and the University of California, Irvine (UCI). Organized by Paolo Sassone-Corsi (UCI) and held at the Beckman Center of the National Academy of Sciences at the UCI campus December 15–16, 2011, this was the first of a series of international conferences on epigenetics dedicated to the scientific community in Southern California. The meeting also served as the official kick off for the newly formed Center for Epigenetics and Metabolism at the School of Medicine, UCI (http://cem.igb.uci.edu). PMID:22414797

  6. A Southern African positron beam

    International Nuclear Information System (INIS)

    Britton, D.T.; Haerting, M.; Teemane, M.R.B.; Mills, S.; Nortier, F.M.; Van der Walt, T.N.

    1997-01-01

    The first stage of a state of the art positron beam, being constructed at the University of Cape Town, is currently being brought into operation. This is the first positron beam on the African continent, as well as being the first positron beam dedicated to solid and surface studies in the southern hemisphere. The project also contains a high proportion of local development, including the encapsulated 22 Na positron source developed by our collaboration. Novel features in the design include a purely magnetic in-line deflector, working in the solenoidal guiding field, to eliminate unmoderated positrons and block the direct line of sight to the source. A combined magnetic projector and single pole probe forming lens is being implemented in the second phase of construction to achieve a spot size of 10 μm without remoderation

  7. The southern cone petroleum market

    International Nuclear Information System (INIS)

    Pisani, W.

    1992-01-01

    The Argentine oil sector has been moving strongly toward complete deregulation since 1989. Price controls on byproducts has been lifted, old petroleum contracts became into concessions, and the state oil company, YPF, is under process of privatization. In this context, the international companies scouting for opportunities can find an important menu of potential investments But here remain some problems connected with this deregulation, too. The lack of a reference crude and product market price is one of them. This paper focuses how to overcome this trouble with the establishment of an institutional market for crude and products, not only for Argentina but also for the entire Southern Cone Region (Argentina, Bolivia, Brazil, Chile, Paraguay and Uruguay), inquiring into the benefits of its creation

  8. Southern company energy storage study :

    Energy Technology Data Exchange (ETDEWEB)

    Ellison, James; Bhatnagar, Dhruv; Black, Clifton; Jenkins, Kip

    2013-03-01

    This study evaluates the business case for additional bulk electric energy storage in the Southern Company service territory for the year 2020. The model was used to examine how system operations are likely to change as additional storage is added. The storage resources were allowed to provide energy time shift, regulation reserve, and spinning reserve services. Several storage facilities, including pumped hydroelectric systems, flywheels, and bulk-scale batteries, were considered. These scenarios were tested against a range of sensitivities: three different natural gas price assumptions, a 15% decrease in coal-fired generation capacity, and a high renewable penetration (10% of total generation from wind energy). Only in the elevated natural gas price sensitivities did some of the additional bulk-scale storage projects appear justifiable on the basis of projected production cost savings. Enabling existing peak shaving hydroelectric plants to provide regulation and spinning reserve, however, is likely to provide savings that justify the project cost even at anticipated natural gas price levels. Transmission and distribution applications of storage were not examined in this study. Allowing new storage facilities to serve both bulk grid and transmission/distribution-level needs may provide for increased benefit streams, and thus make a stronger business case for additional storage.

  9. South African southern ocean research programme

    CSIR Research Space (South Africa)

    SASCAR

    1987-01-01

    Full Text Available This document describes the South African National Antarctic Research Programme's (SANARP) physical, chemical and biological Southern Ocean research programme. The programme has three main components: ecological studies of the Prince Edward Islands...

  10. Arctic and Southern Ocean Sea Ice Concentrations

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Monthly sea ice concentration for Arctic (1901 to 1995) and Southern oceans (1973 to 1990) were digitized on a standard 1-degree grid (cylindrical projection) to...

  11. Southern Forests: a Journal of Forest Science

    African Journals Online (AJOL)

    Southern Hemisphere Forestry Journal. ... The journal particularly encourages contributions from South America, Africa and tropical/subtropical ... Alternative eucalypts for commercial pulpwood production at moderately dry sites in the warm ...

  12. Southern African Development Research Network | IDRC ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    ... to craft policies for fruitful integration into the global economy and inclusive growth. ... The grant will support a broad-based research network, the Southern Africa ... researchers based in regional institutions; transforming selected institutions ...

  13. Southern African Business Review: Editorial Policies

    African Journals Online (AJOL)

    The Southern African Business Review is a refereed and accredited scientific ... Accounting, Public Management, Tourism Management and related fields. ... This journal provides immediate open access to its content on the principle that ...

  14. Introduction to prescribed fires in Southern ecosystems

    Science.gov (United States)

    Thomas A. Waldrop; Scott L. Goodrick

    2012-01-01

    This publication is a guide for resource managers on planning and executing prescribed burns in Southern forests and grasslands. It includes explanations of reasons for prescribed burning, environmental effects, weather, and techniques as well as general information on prescribed burning.

  15. Climate Prediction Center Southern Oscillation Index

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This is one of the CPC?s Monthly Atmospheric and Sea Surface Temperature (SST)Indices. It contains Southern Oscillation Index which is standardized sea level...

  16. Eastern and Southern Africa Seismological Working Group

    International Nuclear Information System (INIS)

    Ogubazghi, G.

    2002-01-01

    Member countries of the Eastern and Southern Africa Seismologica Working Group are listed. The presentation also gives the objectives, activities, date of birth and sponsors of the said ESARSWG. Areas of possible cooperation with CTBTO are indicated

  17. Exploring sustainable transportation for Texas Southern University.

    Science.gov (United States)

    2014-05-01

    Texas Southern University is a commuter campus with students, faculty, and staff traveling from the : Greater Houston area to the university. Over the past few years, the TSU campus has made marked : improvements to move towards a greener more ...

  18. Southern African Journal of Environmental Education

    African Journals Online (AJOL)

    ... knowledge and community based management of wildlife resources: a study of the Mumbwa and Lupande Game Management areas of Zambia. ... Southern African Journal of Environmental Education ... AJOL African Journals Online.

  19. Mental Health Services in Southern Sudan

    African Journals Online (AJOL)

    Siegal_D

    Editorial: Mental Health Services in Southern Sudan – a. Vision for the Future. Major mental illness exists all over the world with a remarkably .... minus one or both parents. ... There he taught and inspired child health professionals from all over.

  20. Southern Hemisphere Ice Limits, 1973-1978

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Weekly Southern Ocean ice limits, have been digitized from U.S. Navy Fleet Weather Facility ice charts, at the Max-Planck Institut fur Meteorologie, Hamburg....

  1. sustainable management of rainforest in southern nigeria

    African Journals Online (AJOL)

    BARTH

    2012-07-23

    Jul 23, 2012 ... predict the stand structures of the most complex tropical rainforest ecosystem in Southern ... matrix R was 0.977, which is the intrinsic rate of natural increase with less than zero. ..... management of renewable resources with.

  2. Southern Great Plains Atmospheric Radiation Measurement Site

    Data.gov (United States)

    Federal Laboratory Consortium — The Southern Great Plains Atmospheric Radiation Measurement Site (SGP-ARM) is the oldest and largest of DOE's Arm sites. It was established in 1992. It consists of...

  3. Leveraging Emerging Technologies in Southern Thailand

    National Research Council Canada - National Science Library

    Valentine, Albert R

    2005-01-01

    .... This thesis examines the history of southern Thailand, including the political factors behind the Malay-Muslim rebellions of the past, the roots of this rebellion back to the era of Patani Raya...

  4. Irreplaceable Acting | Wright | Shakespeare in Southern Africa

    African Journals Online (AJOL)

    Shakespeare in Southern Africa. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 25 (2013) >. Log in or Register to get access to full text downloads.

  5. Two Hamlets | Burnett | Shakespeare in Southern Africa

    African Journals Online (AJOL)

    Shakespeare in Southern Africa. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 23 (2011) >. Log in or Register to get access to full text downloads.

  6. The Southern Forest Futures Project: summary report

    Science.gov (United States)

    David N. Wear; John G. Greis

    2012-01-01

    The Southern Forest Futures Project provides a science-based “futuring” analysis of the forests of the 13 States of the Southeastern United States. With findings organized in a set of scenarios and using a combination of computer models and science synthesis, the authors of the Southern Forest Futures Project examine a variety of possible futures that could shape...

  7. Marketing of organic products in southern Poland

    OpenAIRE

    Kuboń Maciej; Olech Elżbieta

    2018-01-01

    The article presents an outline of the issue concerning formulation of a marketing strategy and the possibility of using the knowledge on consumers' preferences for organic development of farms and their products on the example of southern Poland. The paper analyses the distribution process of organic food in the aspect of developing innovative marketing strategies. The studies were performed in 50 organic farms and on the example of 100 respondents from the region of southern Poland. In the ...

  8. Southern Ocean carbon-wind stress feedback

    Science.gov (United States)

    Bronselaer, Ben; Zanna, Laure; Munday, David R.; Lowe, Jason

    2018-02-01

    The Southern Ocean is the largest sink of anthropogenic carbon in the present-day climate. Here, Southern Ocean pCO2 and its dependence on wind forcing are investigated using an equilibrium mixed layer carbon budget. This budget is used to derive an expression for Southern Ocean pCO2 sensitivity to wind stress. Southern Ocean pCO2 is found to vary as the square root of area-mean wind stress, arising from the dominance of vertical mixing over other processes such as lateral Ekman transport. The expression for pCO2 is validated using idealised coarse-resolution ocean numerical experiments. Additionally, we show that increased (decreased) stratification through surface warming reduces (increases) the sensitivity of the Southern Ocean pCO2 to wind stress. The scaling is then used to estimate the wind-stress induced changes of atmospheric pCO_2 in CMIP5 models using only a handful of parameters. The scaling is further used to model the anthropogenic carbon sink, showing a long-term reversal of the Southern Ocean sink for large wind stress strength.

  9. Silvicultural Considerations in Managing Southern Pine Stands in the Context of Southern Pine Beetle

    Science.gov (United States)

    James M. Guldin

    2011-01-01

    Roughly 30 percent of the 200 million acres of forest land in the South supports stands dominated by southern pines. These are among the most productive forests in the nation. Adapted to disturbance, southern pines are relatively easy to manage with even-aged methods such as clearcutting and planting, or the seed tree and shelterwood methods with natural regeneration....

  10. 77 FR 14351 - New England Fishery Management Council; Public Meeting

    Science.gov (United States)

    2012-03-09

    ... assessments and develop ABC recommendations for the Council for redfish, Georges Bank haddock, Gulf of Maine/Georges Bank windowpane flounder, Southern New England/Mid-Atlantic windowpane flounder, ocean pout... address the emergency. Special Accommodations This meeting is physically accessible to people with...

  11. Indirect taxes on food in Southern Brazil

    Directory of Open Access Journals (Sweden)

    Denize Mirian da Silva

    2013-12-01

    Full Text Available The objective of this paper is to estimate the indirect tax burden on food for ten income classes, based on income and household total expenditure in southern Brazil. Thus it can be seen as indirect taxes on foods affect the monetary income and consumption pattern of households. To reach the objectives proposed, will be used the Pintos-Payeras (2008 model. The database iscomposed by microdata from the Household Budgeting Survey (POF 2008-2009 and the tax regulations of the country and the southern states of Brazil. The results show that indirect taxes on food in Southern Brazil is regressive when based on income and expenditure of household , ie , the poorest people pay proportionately more taxes and have their consumption pattern highest taxed ICMS (Brazilian value added tax is the tax that contributes most to the regressivity.

  12. The genetic prehistory of southern Africa.

    Science.gov (United States)

    Pickrell, Joseph K; Patterson, Nick; Barbieri, Chiara; Berthold, Falko; Gerlach, Linda; Güldemann, Tom; Kure, Blesswell; Mpoloka, Sununguko Wata; Nakagawa, Hirosi; Naumann, Christfried; Lipson, Mark; Loh, Po-Ru; Lachance, Joseph; Mountain, Joanna; Bustamante, Carlos D; Berger, Bonnie; Tishkoff, Sarah A; Henn, Brenna M; Stoneking, Mark; Reich, David; Pakendorf, Brigitte

    2012-01-01

    Southern and eastern African populations that speak non-Bantu languages with click consonants are known to harbour some of the most ancient genetic lineages in humans, but their relationships are poorly understood. Here, we report data from 23 populations analysed at over half a million single-nucleotide polymorphisms, using a genome-wide array designed for studying human history. The southern African Khoisan fall into two genetic groups, loosely corresponding to the northwestern and southeastern Kalahari, which we show separated within the last 30,000 years. We find that all individuals derive at least a few percent of their genomes from admixture with non-Khoisan populations that began ∼1,200 years ago. In addition, the East African Hadza and Sandawe derive a fraction of their ancestry from admixture with a population related to the Khoisan, supporting the hypothesis of an ancient link between southern and eastern Africa.

  13. The Southern Alberta Information Resources (SAIR Project

    Directory of Open Access Journals (Sweden)

    Kathy Crewdson

    2008-06-01

    Full Text Available Abstract Southern Alberta Information Resources (SAIR is a collaborative bibliography of published resources significant to southern Alberta. Objectives and progress with evolving methodology, technology, issues and challenges are explored within the context of the library field. We investigate a collaborative digital library that allows librarians and non-librarians alike to share information on specific topics through MARC records. An outcome of a collaborative digital library is how to create and sustain interest within the library community. Southern Alberta region was selected based on the authors’ familiarity with the region. Some issues and questions remain to be resolved. Digital formats present a number of challenges in terms of selection and presentation. Legal issues relating to technology such as linking and location information have emerged. Basic technical issues remain, such as, how best to update links.

  14. A conspicuous globule in the Southern Coalsack

    International Nuclear Information System (INIS)

    Bok, B.J.; Sim, M.E.; Hawarden, T.G.

    1977-01-01

    It is stated that the Southern Coalsack is the finest dark nebula in the Southern Milky Way. Two photographs are here shown taken in October 1976 with the SRC 1.2 m Schmidt telescope at the Siding Spring Observatory, Australia. These show the whole of the Coalsack in all its beauty, and its features are discussed. It is shown how much can be learned by simple star counting techniques. A large number of potential globules are indicated, some of which are described. (U.K.)

  15. New Moho map of southern Norway

    DEFF Research Database (Denmark)

    Stratford, Wanda Rose; Thybo, Hans; Faleide, J.-I.

    2009-01-01

    A recent seismic refraction study across southern Norway has revealed that the up to 2469 m high Southern Scandes Mountains are not isostatically compensated by a thick crust. Rather, the Moho depths are close to average for continental crust with elevations of ~1 km. Evidence from new seismic data......, low densities in the upper-mantle or mantle dynamics, is likely. The relationship between topography and Moho depth breaks down for the Oslo Graben and the Fennoscandian Shield to the east and north. High density lower crustal rocks below Oslo Graben and increasing crust and lithospheric thicknesses...

  16. Natural road construction materials of Southern Africa

    CSIR Research Space (South Africa)

    Weinert, HH

    1980-01-01

    Full Text Available respectively. p. 210. column 4: Insert Notre in rect311glc directly below heading Matrix. p. 240, paragraph 5, line 4: J 770 should read I 700. I ~I -~ r THE NATURAL ROAD CONSTRUCTION MATERIALS OF SOUTHERN AFRICA ACADEMICA I PRETORIA ICAPE TOWN ISBN... and the author acknowledges gratefully the valuable assistance and the trouble taken by the following persons: Southern African road authorities: Mr W. J. Biesenbach, Mr H. K. Geel, Mr C. L. Laubscher, Mr G. P. Marais, Mr R. L. Mitchell, Mr I. P. A. Smit, Mr C...

  17. The Southern Kalahari: a potential new dust source in the Southern Hemisphere?

    International Nuclear Information System (INIS)

    Bhattachan, Abinash; D’Odorico, Paolo; Baddock, Matthew C; Zobeck, Ted M; Okin, Gregory S; Cassar, Nicolas

    2012-01-01

    Most sources of atmospheric dust on Earth are located in the Northern Hemisphere. The lower dust emissions in the Southern Hemisphere in part limit the supply of micronutrients (primarily soluble iron) to the Southern Ocean, thereby constraining its productivity. Climate and land use change can alter the current distribution of dust source regions on Earth. Can new dust sources be activated in the Southern Hemisphere? Here we show that vegetation loss and dune remobilization in the Southern Kalahari can promote dust emissions comparable to those observed from major contemporary dust sources in the Southern African region. Dust generation experiments support the hypothesis that, in the Southern Kalahari, aeolian deposits that are currently mostly stabilized by savanna vegetation are capable of emitting substantial amounts of dust from interdune areas. We show that dust from these areas is relatively rich in soluble iron, an important micronutrient for ocean productivity. Trajectory analyses show that dust from the Kalahari commonly reaches the Southern Ocean and could therefore enhance its productivity. (letter)

  18. Biochemical characteristics of four marine fish skins in Korea.

    Science.gov (United States)

    Cho, Jae-Kwon; Jin, Young-Guk; Rha, Sung-Ju; Kim, Seon-Jae; Hwang, Jae-Ho

    2014-09-15

    In this study, we investigated the biochemical characteristics of the fish skins of four industrial species: olive flounder (Paralichthys olivaceus), black rockfish (Sebastes schlegeli), sea bass (Lateolabrax maculatus) and red sea bream (Pagrus major). There is high domestic demand in Korea for farming of these fish for human consumption. Crude protein contents in the skin of these fish ranged from 73% to 94% by dry weight; this was in part due to a high content of the structural protein, collagen. Among the four species, olive flounder had the thickest dermal and epidermal layers in the dorsal skin. This species was also associated with the highest extraction ratio of acid-soluble collagen. We also examined whether fish skin could be a cost-effective alternative to current fish meal sources. Our analysis indicates that, when supplemented with additional fish oils and essential amino acids, fish skin is a viable alternative for fish meal formulations. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Southern Fireworks above ESO Telescopes

    Science.gov (United States)

    1999-05-01

    New Insights from Observations of Mysterious Gamma-Ray Burst International teams of astronomers are now busy working on new and exciting data obtained during the last week with telescopes at the European Southern Observatory (ESO). Their object of study is the remnant of a mysterious cosmic explosion far out in space, first detected as a gigantic outburst of gamma rays on May 10. Gamma-Ray Bursters (GRBs) are brief flashes of very energetic radiation - they represent by far the most powerful type of explosion known in the Universe and their afterglow in optical light can be 10 million times brighter than the brightest supernovae [1]. The May 10 event ranks among the brightest one hundred of the over 2500 GRB's detected in the last decade. The new observations include detailed images and spectra from the VLT 8.2-m ANTU (UT1) telescope at Paranal, obtained at short notice during a special Target of Opportunity programme. This happened just over one month after that powerful telescope entered into regular service and demonstrates its great potential for exciting science. In particular, in an observational first, the VLT measured linear polarization of the light from the optical counterpart, indicating for the first time that synchrotron radiation is involved . It also determined a staggering distance of more than 7,000 million light-years to this GRB . The astronomers are optimistic that the extensive observations will help them to better understand the true nature of such a dramatic event and thus to bring them nearer to the solution of one of the greatest riddles of modern astrophysics. A prime example of international collaboration The present story is about important new results at the front-line of current research. At the same time, it is also a fine illustration of a successful collaboration among several international teams of astronomers and the very effective way modern science functions. It began on May 10, at 08:49 hrs Universal Time (UT), when the Burst

  20. Current Writing: Text and Reception in Southern Africa: Advanced ...

    African Journals Online (AJOL)

    Current Writing: Text and Reception in Southern Africa: Advanced Search. Journal Home > Current Writing: Text and Reception in Southern Africa: Advanced Search. Log in or Register to get access to full text downloads.

  1. Southern Africa Journal of Education, Science and Technology ...

    African Journals Online (AJOL)

    Southern Africa Journal of Education, Science and Technology: Journal Sponsorship. Journal Home > About the Journal > Southern Africa Journal of Education, Science and Technology: Journal Sponsorship. Log in or Register to get access to full text downloads.

  2. Southern Africa Journal of Education, Science and Technology: Site ...

    African Journals Online (AJOL)

    Southern Africa Journal of Education, Science and Technology: Site Map. Journal Home > About the Journal > Southern Africa Journal of Education, Science and Technology: Site Map. Log in or Register to get access to full text downloads.

  3. Prevalence of Risk Factors for Coronary Artery Disease in Southern ...

    African Journals Online (AJOL)

    CAD) in Southern Punjab, Pakistan. Methods: A cross-sectional survey was conducted for the prevalence of risk factors for CAD among 200 patients admitted at the different hospitals of Southern Punjab, Pakistan from December 2012 to April ...

  4. Climate and southern Africa's water-energy-food nexus

    CSIR Research Space (South Africa)

    Conway, D

    2015-09-01

    Full Text Available be strengthened for this purpose: the Southern African Development Community, the Southern African Power Pool and trade of agricultural products amounting to significant transfers of embedded water....

  5. 78 FR 34151 - Notice of Public Hearing: Norfolk Southern Corporation

    Science.gov (United States)

    2013-06-06

    ... DEPARTMENT OF TRANSPORTATION Federal Railroad Administration [Docket Number FRA-2012-0087] Notice of Public Hearing: Norfolk Southern Corporation The Norfolk Southern Corporation (NS) petitioned the Federal Railroad Administration (FRA) seeking the approval of the proposed discontinuance of automatic...

  6. Comparing internal and external drivers in the southern Benguela ...

    African Journals Online (AJOL)

    Comparing internal and external drivers in the southern Benguela and the southern and ... during the model fitting: internal forcing by means of the trophic flow controls ... and two kinds of external forcing, namely fishing and the environment.

  7. The lithospheric mantle below southern West Greenland

    DEFF Research Database (Denmark)

    Sand, Karina Krarup; Waight, Tod Earle; Pearson, D. Graham

    2009-01-01

    Geothermobarometry of primarily garnet lherzolitic xenoliths from several localities in southern West Greenland is applied to address the diamond potential, pressure and temperature distribution and the stratigraphy of the subcontinental lithospheric mantle ~600 Ma ago. The samples are from kimbe...... into the reworked Archean North of the Naqssugtoqidian deformation front....

  8. Sequence crystallization during isotherm evaporation of southern ...

    African Journals Online (AJOL)

    Southern Algerian's natural brine sampled from chott Baghdad may be a source of mineral salts with a high economic value. These salts are recoverable by simple solar evaporation. Indeed, during isothermal solar evaporation, it is possible to recover mineral salts and to determine the precipitation sequences of different ...

  9. Optical observations of southern planetary nebula candidates

    NARCIS (Netherlands)

    VandeSteene, GC; Sahu, KC; Pottasch, [No Value

    1996-01-01

    We present H alpha+[NII] images and low resolution spectra of 16 IRAS-selected, southern planetary nebula candidates previously detected in the radio continuum. The H alpha+[NII] images are presented as finding charts. Contour plots are shown for the resolved planetary nebulae. From these images

  10. First Hemolivia from southern Africa: reassigning chelonian ...

    African Journals Online (AJOL)

    To date, only a single species of Hemolivia, Hemolivia mauritanica (Sergent & Sergent, 1904), has been described from African terrestrial tortoises. Although various haemogregarines have been described from southern African terrapins and tortoises, including species from the genus Haemogregarina and one from the ...

  11. Southern Europeans in France: Invisible Migrants?

    NARCIS (Netherlands)

    Eremenko, T.; El Qadim, N.; Steichen, E.; Lafleur, J.-M.; Stanek, M.

    2016-01-01

    France fared relatively well at the start of the current economic crisis, but has experienced low economic growth and high unemployment rates in the recent years. As a result it has been a less popular destination with Southern Europeans and EU migrants in general in search of economic

  12. On some birds from southern Mexico

    NARCIS (Netherlands)

    Mees, G.F.

    1970-01-01

    In the years 1962/64 our museum purchased from Mr. Otto Epping, now of Pittsburgh, U.S.A., a collection of 700 bird-specimens from southern Mexico (mainly from Vera Cruz and Oaxaca, a few specimens from Puebla). As our museum was poorly provided with material from Mexico, this was a very welcome

  13. Structural Change in Southern Softwood Stumpage Markets

    Science.gov (United States)

    Douglas R. Carter

    1998-01-01

    The potential for structural change in southern stumpage market models has impacts on not only our basic understanding of those markets, but also on harvest, inventory and price projections, and related policy. In this paper, we test for structural change in both sawtimber and pulpwood softwood stumpage markets in the U.S. South over the period 1950-1994. Test...

  14. Invasive species in southern Nevada [Chapter 4

    Science.gov (United States)

    Mathew L. Brooks; Steven M. Ostoja; Jeanne C. Chambers

    2013-01-01

    Southern Nevada contains a wide range of topographies, elevations, and climatic zones emblematic of its position at the ecotone between the Mojave Desert, Great Basin, and Colorado Plateau ecoregions. These varied environmental conditions support a high degree of biological diversity (Chapter 1), but they also provide opportunities for a wide range of invasive species...

  15. Southern Forest Resource Assessment - Summary Report

    Science.gov (United States)

    David N. Wear; John G. Greis

    2002-01-01

    The Southern Forest Resource Assessment was initiated in 1999 as a result of concerns raised by natural resource managers, the science community, and the public regarding the status and likely future of forests in the South. These included changes to the region’s forests brought about by rapid urbanization, increasing timber demand, increasing numbers of...

  16. Economic Impacts of the Southern Pine Beetle

    Science.gov (United States)

    John M. Pye; Thomas P. Holmes; Jeffrey P. Prestemon; David N. Wear

    2011-01-01

    This paper provides an overview of the timber economic impacts of the southern pine beetle (SPB). Although we anticipate that SPB outbreaks cause substantial economic losses to households that consume the nonmarket economic services provided by healthy forests, we have narrowly focused our attention here on changes in values to timber growers and wood-products...

  17. Community management of coastal resources, southern Thailand

    OpenAIRE

    Chansnoh, P.

    1993-01-01

    The involvement of communities with the assistance and support of government and non government organizations on the management of the coastal resources in Southern Thailand are discussed. The 3 most important resources, mangrove, seagrass and coral, create a complex coastal ecology. Several man-made activities causing the deterioration of this resources are also presented.

  18. Gold deposits of the southern Piedmont

    Science.gov (United States)

    Pardee, J.T.; Park, C.F.

    1948-01-01

    This report deals chiefly with the gold mines in the Southern Appalachian gold belt whose workings were accessible at the time of examination, but it also · summarizes available information concerning many mines that were not accessible. Most of the mines lie within a belt, 10 to 100 miles wide, that extends

  19. Assessing estuarine biota in southern California

    Science.gov (United States)

    Kevin D. Lafferty

    2005-01-01

    In southern California, most estuarine wetlands are gone, and what little habitat remains is degraded. For this reason, it is often of interest to assess the condition of estuaries over time, such as when determining the success of a restoration project. To identify impacts or opportunities for restoration, we also may want to know how a particular estuary, or area...

  20. Marketing University Education: The Southern African Experience.

    Science.gov (United States)

    Maringe, Felix; Foskett, Nick

    2002-01-01

    Examined the perceptions of university marketers in southern Africa. Found a varying awareness of the significance of marketing, with more mature institutions exhibiting more developed marketing orientations. Strategies ranged from marketing as public relations to marketing as sales, with universities in South Africa the only ones demonstrating a…

  1. Soyabeans and sustainable agriculture in southern Africa

    NARCIS (Netherlands)

    Giller, K.E.; Murwira, M.S.; Dhliwayo, D.K.C.; Mafongoya, P.L.; Mpepereki, S.

    2011-01-01

    Maize is the dominant staple crop across most of southern Africa - it is so dominant in some areas that more than 80 per cent of the smallholder land area is planted with maize. Soyabean was identified as the crop with a potential to address the need for diversifying the cropping systems, which

  2. Field guide to trees of Southern Africa

    OpenAIRE

    Rishan Singh

    2012-01-01

    The mainland region of Africa is Southern Africa because it is considered to be robust with an estimate of around 1700 tree species that are native and a couple 100 more that are alien, but have become accustomed to the natural environment; invading, penetrating and replacing vegetation.

  3. Zoogeography of the southern African echinoderm fauna

    African Journals Online (AJOL)

    1988-04-17

    Apr 17, 1988 ... Pertinent features of the oceanography of southern Africa are reviewed and an analysis of the echinoderm fauna in relation to the genera] ..... five extant echinoderm classes (all species). Crinoids. Asteroids Ophiuroids .... Australia and New Zealand, which are included with. R eprod u ced by Sabin et G.

  4. Southern forests: Yesterday, today, and tomorrow

    Science.gov (United States)

    R. Neil Sampson

    2004-01-01

    In the 20th century, southern forests changed dramatically. Those changes pale, however, when compared to what happened to the people of the region. In addition to growing over fourfold in numbers, the South's population has urbanized, globalized, and intellectualized in 100 years. Rural and isolated in the 19th century, they are today urban and cosmopolitan. One...

  5. Rabies vaccine and neuraxial anaesthesia | Rewari | Southern ...

    African Journals Online (AJOL)

    Southern African Journal of Anaesthesia and Analgesia. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 16, No 5 (2010) >. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register · Download this PDF file. The PDF ...

  6. Towards a Southern African English Defining Vocabulary

    African Journals Online (AJOL)

    user

    of parameters, such as avoiding synonyms and antonyms, to determine which words are necessary to write definitions in a concise and simple way. It has been found that existing defining vocabularies lack certain words that would make definitions more accessible to southern African learners, and therefore there is a need ...

  7. Risk Assessment for the Southern Pine Beetle

    Science.gov (United States)

    Andrew Birt

    2011-01-01

    The southern pine beetle (SPB) causes significant damage (tree mortality) to pine forests. Although this tree mortality has characteristic temporal and spatial patterns, the precise location and timing of damage is to some extent unpredictable. Consequently, although forest managers are able to identify stands that are predisposed to SPB damage, they are unable to...

  8. A MEAT PRODUCTION STRATEGYFOR SOUTHERN AFRICA

    African Journals Online (AJOL)

    the task of formulating a rneat prclduction strategy fclr. Southern Africa .... Ch ickcn. '55. '5? '59. '61. 6l. '65. '61. '69. '71. '7-f is. "47. Fig. I Edible protein deived from various animal products ...... of the effort and finance expended on the original.

  9. Globalisation, transport and HIV | Andrews | Southern African ...

    African Journals Online (AJOL)

    No Abstract Southern African Journal of HIV Medicine Vol. 5 (4) 2004: 41-44. Full Text: EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT · AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL ...

  10. Globalisation, transport and HIV | Andrews | Southern African ...

    African Journals Online (AJOL)

    Southern African Journal of HIV Medicine. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 5, No 4 (2004) >. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register · Download this PDF file. The PDF file you selected ...

  11. Southern Nevada ecosystem stressors [Chapter 2

    Science.gov (United States)

    Burton K. Pendleton; Jeanne C. Chambers; Mathew L. Brooks; Steven M. Ostoja

    2013-01-01

    Southern Nevada ecosystems and their associated resources are subject to a number of global and regional/local stressors that are affecting the sustainability of the region. Global stressors include elevated carbon dioxide (CO2) concentrations and associated changes in temperature and precipitation patterns and amounts, solar radiation, and nutrient cycles (Smith and...

  12. Anatomical characteristics of southern pine stemwood

    Science.gov (United States)

    Elaine T. Howard; Floyd G. Manwiller

    1968-01-01

    To obtain a definitive description of the wood and anatomy of all 10 species of southern pine, juvenile, intermediate, and mature wood was sampled at three heights in one tree of each species and examined under a light microscope. Photographs and three-dimensional drawings were made to illustrate the morphology. No significant anatomical differences were found...

  13. Home garden system dynamics in Southern Ethiopia

    NARCIS (Netherlands)

    Mellisse, Beyene Teklu; Ven, van de Gerrie W.J.; Giller, Ken E.; Descheemaeker, Katrien

    2017-01-01

    Home gardens in southern Ethiopia are regarded as efficient farming systems, allowing interactions and synergies between crop, tree and livestock components. However, these age-old traditional home gardens are evolving rapidly in response to changes in both the socio-economic and biophysical

  14. Radio broadcasting for sustainable development in southern ...

    African Journals Online (AJOL)

    Patrick O. Waeber and Yvonne Orengo

    2008-12-01

    Dec 1, 2008 ... ABSTRACT. The Millennium Development Goals have been written into the Madagascar Road Map (2007 - 2012) in order to improve the Malagasy social, economic and environmental situation. The Andrew Lees Trust Radio Broadcasting Project in southern. Madagascar has been set up to alleviate ...

  15. Resource reviews | Naude | Southern African Journal of ...

    African Journals Online (AJOL)

    Petro Naude. Walk through the wilderness by Don Richards and Clive. Walker. Published by Endangered Wildlife Trust and Wilderness Trust of Southern Africa, Johannesburg. Second (revised) edition 1986. 146 pp., photographs, maps, charts and line drawings. Price R9,95. 2. Pat Irwin. Trout in South Africa edited by P.H. ...

  16. PLANT DERMATITIS IN THE SOUTHERN TRANSVAAL*

    African Journals Online (AJOL)

    le bush stinging-nettle may be encountered in bush ps in Southern Transvaal veld. vo patients with seasonal rashes of the face suggestive lant dermatitis were seen in this series, but no definite es were found. MANAGEMENT. ~rential Diagnosis mditions seen in the survey period which could be used with plant dermatitis ...

  17. Faulkner's Southern belle - myth or reality?

    Directory of Open Access Journals (Sweden)

    Nataša Intihar Klančar

    2011-12-01

    Full Text Available The article deals with heroines of William Faulkner's novels Light in August, Absalom, Absalom!, The Sound and the Fury, The Unvanquished, The Town and his short story "A Rose for Emily". The Southern belle features as a recurring character in Faulkner's fiction, her fragility, modesty, weakness yet strength, beauty, sincerity, generous nature, status and her fall from innocence comprise her central characteristics. Confronted with various expectations of Southern society and with the hardships of war, the belle is faced with many obstacles and challenges. Faulkner's heroines face a wide array of problems that prevent them from being and/or remaining a Southern belle. Let us name a few: Lena's inappropriate social status, Joanna's wrong roots, Mrs. Hightower's inability to fulfill her duties as the minister's wife, Ellen's miserable marriage, Judith's sad love life, Rosa's feelings of inferiority and humiliation, Mrs. Compson's failure as a mother, Caddy's weak rebellion against male convention, Drusilla's male characteristics, Linda's unrequited love and Emily's dark secret, to name a few. Through these characters and their destinies Faulkner shows a decaying South whose position has changed considerably over the years. Can the Southern belle save it? Can she save herself?

  18. Review of Southern African Studies: Submissions

    African Journals Online (AJOL)

    Author Guidelines. 1. Every manuscript should be accompanied with a statement that it has not been submitted for publication elsewhere. 2. The Review of Southern African Studies prefers articles which cut across disciplinary boundaries. Articles with narrow foci and incomprehensible to people outside those disciplines ...

  19. Review of Southern African Studies: Editorial Policies

    African Journals Online (AJOL)

    Editorial Board. Prof. R.C. Leduka Institute of Southern African Studies, NUL. Dr. F. Baffoe Baffoe and Associates, Maseru. Prof. Q. Chakela National University of Lesotho. Prof. L.B.B.J. Machobane Machobane and Associates, Maseru. Prof. E.M. Sebatane National University of Lesotho. Dr. E. Obioha National University of ...

  20. Early German Plans for a Southern Observatory

    Science.gov (United States)

    Wolfschmidt, Gudrun

    As early as the 18th and 19th centuries, French and English observers were active in South Africa. Around the beginning of the 20th century the Heidelberg astronomer Max Wolf (1863-1932) proposed a southern observatory. In 1907 Hermann Carl Vogel (1841-1907), director of the Astrophysical Observatory Potsdam, suggested a southern station in Spain. His ideas for building an observatory in Windhuk for photographing the sky and measuring the solar constant were taken over by the Göttingen astronomers. In 1910 Karl Schwarzschild (1873-1916), after having visited the observatories in America, pointed out the usefulness of an observatory in South West Africa, where it would have better weather than in Germany and also give access to the southern sky. Seeing tests were begun in 1910 by Potsdam astronomers, but WW I stopped the plans. In 1928 Erwin Finlay-Freundlich (1885-1964), inspired by the Hamburg astronomer Walter Baade (1893-1960), worked out a detailed plan for a southern observatory with a reflecting telescope, spectrographs and an astrograph with an objective prism. Paul Guthnick (1879-1947), director of the Berlin observatory, in cooperation with APO Potsdam and Hamburg, made a site survey to Africa in 1929 and found the conditions in Windhuk to be ideal. Observations were started in the 1930s by Berlin and Breslau astronomers, but were stopped by WW II. In the 1950s, astronomers from Hamburg and The Netherlands renewed the discussion in the framework of European cooperation, and this led to the founding of ESO in 1963, as is well described by Blaauw (1991). Blaauw, Adriaan: ESO's Early History. The European Southern Observatory from Concept to Reality. Garching bei München: ESO 1991.

  1. Southern Forest Resource Assessment and Linkages to the National RPA

    Science.gov (United States)

    Fredrick Cubbage; Jacek Siry; Steverson Moffat; David N. Wear; Robert Abt

    1998-01-01

    We developed a Southern Forest Resource Assessment Consortium (SOFAC) in 1994, which is designed to enhance our capabilities to analyze and model the southern forest and timber resources. Southern growth and yield analyses prepared for the RPA via SOFAC indicate that substantial increases in timber productivity can occur given current technology. A survey about NIPF...

  2. Southern Voice State of the Sustainable Development Goals (SDGs ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    The Southern Voice on post-MDG International Development Goals (Southern ... conceived by the Southern Voice network that will generate and disseminate ... this project will help expand ownership of the SDGs in case study countries and ... think tanks as effective interlocutors between national capitals, regional hubs, ...

  3. Straight studs are produced from southern pine cordwood

    Science.gov (United States)

    Peter Koch

    1967-01-01

    A Process for converting southern pine veneer cores into 8-foot 2 by 4's of SPIB Stud grade and better has been developed at the Alexandria, Louisiana, Utilization Laboratory of the Southern Forest Experiment Station. The research leading to this development suggests that a similiar process would be practical for converting 8-foot southern pine cordwood into studs...

  4. Southern hardwood forestry group going strong after 50 years

    Science.gov (United States)

    Brian Roy Lockhart; Steve Meadows; Jeff Portwood

    2005-01-01

    On November 15,200 1, the Southern Hardwood Forestry Group (referred to as the Group) met at the U.S. Forest Service Southern Research Station's Southern Hardwoods Laboratory in Stoneville, hlississippi to celebrate the Group's 50th anniversary. About 130 members and guests attended to celebrate the 50th anniversary of the Group and to honor its charter...

  5. 77 FR 15994 - Southern Region Recreation Resource Advisory Committee

    Science.gov (United States)

    2012-03-19

    ... DEPARTMENT OF AGRICULTURE Forest Service Southern Region Recreation Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting via teleconference. SUMMARY: The Southern Region... and requests to Southern Region Recreation RAC, Caroline Mitchell, P.O. Box 1270, Hot Springs, AR...

  6. Comparisons of invasive plants in southern Africa originating from southern temperate, northern temperate and tropical regions

    Directory of Open Access Journals (Sweden)

    L. Henderson

    2006-08-01

    Full Text Available A subset of invasive alien plant species in southern Africa was analysed in terms of their history of introduction, rate of spread, countries/region of origin, taxonomy, growth forms, cultivated uses, weed status and current distribution in southern Africa, and comparisons made of those originating from south of the tropic of Capricorn, north of the tropic of Cancer and from the tropics. The subset of 233 species, belonging to 58 families, includes all important declared species and some potentially important species. Almost as many species originate from temperate regions (112 as from the tropics (121. Most southern temperate species came from Australia (28/36, most tropical species from tropical America (92/121 and most northern temperate species from Europe (including the Mediterranean and Asia (58/76. Transformers account for 33% of  all species. More transformers are of tropical origin (36 than of northern temperate (24 and southern temperate origin (18. However. 50% of southern temperate species are transformers, compared to 32% of northern temperate and 29% of tropical species. Southern temperate transformer species are mainly woody trees and shrubs that were established on a grand scale as silvicultural crops, barriers (hedges, windbreaks and screens and cover/binders. Most aquatics, herbs, climbers and succulent shrubs an. trom the tropics. Ornamentals are the single largest category of plants from all three regions, the tropics having contributed twice as many species as temperate regions.

  7. Support for the Confederate Battle Flag in the Southern United States: Racism or Southern Pride?

    Directory of Open Access Journals (Sweden)

    Joshua D. Wright

    2017-05-01

    Full Text Available Supporters of the Confederate battle flag often argue that their support is driven by pride in the South, not negative racial attitudes. Opponents of the Confederate battle flag often argue that the flag represents racism, and that support for the flag is an expression of racism and an attempt to maintain oppression of Blacks in the Southern United States. We evaluate these two competing views in explaining attitudes toward the Confederate battle flag in the Southern United States through a survey of 526 Southerners. In the aggregate, our latent variable model suggests that White support for the flag is driven by Southern pride, political conservatism, and blatant negative racial attitudes toward Blacks. Using cluster-analysis we were able to distinguish four distinct sub-groups of White Southerners: Cosmopolitans, New Southerners, Traditionalists, and Supremacists. The greatest support for the Confederate battle flag is seen among Traditionalists and Supremacists; however, Traditionalists do not display blatant negative racial attitudes toward Blacks, while Supremacists do. Traditionalists make up the majority of Confederate battle flag supporters in our sample, weakening the claim that supporters of the flag are generally being driven by negative racial attitudes toward Blacks.

  8. 76 FR 35508 - Alabama Southern Railroad, L.L.C.-Temporary Trackage Rights Exemption-Norfolk Southern Railway...

    Science.gov (United States)

    2011-06-17

    ... Railroad, L.L.C.--Temporary Trackage Rights Exemption--Norfolk Southern Railway Company Norfolk Southern... grant nonexclusive overhead temporary trackage rights to Alabama Southern Railroad, L.L.C. (ABS) over a... http://www.stb.dot.gov . Decided: June 13, 2011. By the Board. Rachel D. Campbell, Director, Office of...

  9. The Southern Nevada Agency Partnership Science and Research Synthesis: Science to support land management in Southern Nevada

    Science.gov (United States)

    Jeanne C. Chambers; Matthew L. Brooks; Burton K. Pendleton; Carol B. Raish

    2013-01-01

    This synthesis provides information related to the Southern Nevada Agency Partnership (SNAP) Science and Research Strategy Goal 1 - to restore, sustain and enhance southern Nevada’s ecosystems - and Goal 2 - to provide for responsible use of southern Nevada’s lands in a manner that preserves heritage resources and promotes an understanding of human interaction with the...

  10. The Southern Nevada Agency Partnership Science and Research Synthesis: Science to support land management in Southern Nevada - Executive Summary

    Science.gov (United States)

    Jeanne C. Chambers; Matthew L. Brooks; Burton K. Pendleton; Carol B. Raish

    2013-01-01

    This synthesis provides information related to the Southern Nevada Agency Partnership (SNAP) Science and Research Strategy Goal 1 - to restore, sustain and enhance southern Nevada’s ecosystems - and Goal 2 - to provide for responsible use of southern Nevada’s lands in a manner that preserves heritage resources and promotes an understanding of human interaction with the...

  11. Reemergence of Dengue in Southern Texas, 2013

    Science.gov (United States)

    Thomas, Dana L.; Santiago, Gilberto A.; Abeyta, Roman; Hinojosa, Steven; Torres-Velasquez, Brenda; Adam, Jessica K.; Evert, Nicole; Caraballo, Elba; Hunsperger, Elizabeth; Muñoz-Jordán, Jorge L.; Smith, Brian; Banicki, Alison; Tomashek, Kay M.; Gaul, Linda

    2016-01-01

    During a dengue epidemic in northern Mexico, enhanced surveillance identified 53 laboratory-positive cases in southern Texas; 26 (49%) patients acquired the infection locally, and 29 (55%) were hospitalized. Of 83 patient specimens that were initially IgM negative according to ELISA performed at a commercial laboratory, 14 (17%) were dengue virus positive by real-time reverse transcription PCR performed at the Centers for Disease Control and Prevention. Dengue virus types 1 and 3 were identified, and molecular phylogenetic analysis demonstrated close identity with viruses that had recently circulated in Mexico and Central America. Of 51 household members of 22 dengue case-patients who participated in household investigations, 6 (12%) had been recently infected with a dengue virus and reported no recent travel, suggesting intrahousehold transmission. One household member reported having a recent illness consistent with dengue. This outbreak reinforces emergence of dengue in southern Texas, particularly when incidence is high in northern Mexico. PMID:27191223

  12. Emerging arboviral human diseases in Southern Europe.

    Science.gov (United States)

    Papa, Anna

    2017-08-01

    Southern Europe is characterized by unique landscape and climate which attract tourists, but also arthropod vectors, some of them carrying pathogens. Among several arboviral diseases that emerged in the region during the last decade, West Nile fever accounted for high number of human cases and fatalities, while Crimean-Congo hemorrhagic fever expanded its geographic distribution, and is considered as a real threat for Europe. Viruses evolve rapidly and acquire mutations making themselves stronger and naive populations more vulnerable. In an effort to tackle efficiently the emerging arboviral diseases, preparedness and strategic surveillance are needed for the early detection of the pathogen and containment and mitigation of probable outbreaks. In this review, the main human arboviral diseases that emerged in Southern Europe are described. © 2017 Wiley Periodicals, Inc.

  13. Marketing of organic products in southern Poland

    Directory of Open Access Journals (Sweden)

    Kuboń Maciej

    2018-01-01

    Full Text Available The article presents an outline of the issue concerning formulation of a marketing strategy and the possibility of using the knowledge on consumers' preferences for organic development of farms and their products on the example of southern Poland. The paper analyses the distribution process of organic food in the aspect of developing innovative marketing strategies. The studies were performed in 50 organic farms and on the example of 100 respondents from the region of southern Poland. In the opinion of the surveyed representatives of the organic food producers, a competitive advantage of their offer depends the most on the health values, brand, reputation, and taste. Moreover, information on products and the form and place of their sale are significant. The analysis shows that the knowledge is the most eagerly obtained from the Internet. Thus, producers should publish their profiles and pages on social media and business portals.

  14. Treaty aids 'detensioning of southern Africa'

    International Nuclear Information System (INIS)

    Stumpf, W.

    1991-01-01

    South Africa's accession to the Treaty on the Non-Proliferation of Nuclear Weapons is a significant step forward in trading non-weapons related nuclear technology with other world powers. In an interview with Dr Waldo Stumpf, chief executive officer of the Atomic Energy Corporation of South Africa, the impact of this decision on the Corporation and the whole of southern Africa is discussed

  15. Photometry of Southern Hemisphere red dwarf stars

    Science.gov (United States)

    Weistrop, D.

    1980-01-01

    Results are presented for a photometric investigation of a spectroscopically selected sample of red dwarf stars in the Southern Hemisphere. Absolute magnitudes and distances for the stars are estimated from broadband red colors. Three stars which may be subluminous are identified, as are several stars which may be within 25 pc. The tangential velocity and velocity dispersion of the sample are similar to values found in other studies of nearby late-type stars.

  16. Entrepreneurship and Income Inequality in Southern Ethiopia

    OpenAIRE

    Kimhi, Ayal

    2009-01-01

    This paper uses inequality decomposition techniques in order to analyse the consequences of entrepreneurial activities to household income inequality in southern Ethiopia. A uniform increase in entrepreneurial income reduces per capita household income inequality. This implies that encouraging rural entrepreneurship may be favourable for both income growth and income distribution. Such policies could be particularly successful if directed at the low-income, low-wealth, and relatively uneducat...

  17. Southern Woods-Burners: A Descriptive Analysis

    Science.gov (United States)

    M.L. Doolittle; M.L. Lightsey

    1979-01-01

    About 40 percent of the South's nearly 60,000 wildfires yearly are set by woods-burners. A survey of 14 problem areas in four southern States found three distinct sets of woods-burners. Most active woods-burners are young, white males whose activities are supported by their peers. An older but less active group have probably retired from active participation but...

  18. Aspidonepsis (Asclepiadaceae, a new southern African genus

    Directory of Open Access Journals (Sweden)

    A. Nicholas

    1992-10-01

    Full Text Available Aspidonepsis, an endemic southern African genus, is described and compared to the closely allied genus Aspidoglossum. This newly described genus is composed of two subgenera, Aspidonepsis and Unguilobium. consisting of three and two species respectively.  Asclepias diploglossa, A. flava, A. cognata and A. reneensis are transferred to Aspidonepsis. and A. shebae is newly described. All species are discussed, illustrated and a key is given to aid in their identification.

  19. Southern Ocean Phytoplankton in a Changing Climate

    OpenAIRE

    Deppeler, Stacy L.; Davidson, Andrew T.

    2017-01-01

    Phytoplankton are the base of the Antarctic food web, sustain the wealth and diversity of life for which Antarctica is renowned, and play a critical role in biogeochemical cycles that mediate global climate. Over the vast expanse of the Southern Ocean (SO), the climate is variously predicted to experience increased warming, strengthening wind, acidification, shallowing mixed layer depths, increased light (and UV), changes in upwelling and nutrient replenishment, declining sea ice, reduced sal...

  20. Southern hemisphere searches for short period pulsars

    International Nuclear Information System (INIS)

    Manchester, R.N.

    1984-01-01

    Two searches of the southern sky for short period pulsars are briefly described. The first, made using the 64-m telescope at Parkes, is sensitive to pulsars with periods greater than about 10 ms and the second, made using the Molonglo radio telescope, has sensitivity down to periods of about 1.5 ms. Four pulsars were found in the Parkes survey and none in the Molonglo survey, although analysis of the latter is as yet incomplete. 10 references, 1 figure, 2 tables

  1. Southern Africa’s Transnational Threats

    Science.gov (United States)

    2013-04-01

    private sectors lends evidence to sophisticated criminal activities.”5 As regional organizations contemplated on how to prevent and/or deter these crimes...distribution of Cannabis (also known as marijuana) is prevalent throughout Southern Africa. In most incidences, Cannabis profits dwarfs local income...had its source in Tanzania and Tanzanians have identified markets for their fake US dollars in South Africa. Cannabis South Africa, Lesotho

  2. Contemporary Russia Policy for the Southern Caucasus

    Directory of Open Access Journals (Sweden)

    Karen P. Marabyan

    2014-01-01

    Full Text Available The bilateral relations between Russia on the one hand, and the countries of the Southern Caucasus region on the other hand are examined in the article. The main directions of Russian policy in the Southern Caucasus region are examined there. The article focusses attention to the value of the Southern Caucasus region for Russia and also to the key role of Russia in the Southern Caucasus region. The overview of Russian-Azerbaijani, Russian-Armenian and Russian- Georgian relations is given in the article. Russian relations with unrecognized Nagorno-Karabakh Republic and with partially recognized Abkhazia and South Ossetia are examined in the context of above-mentioned bilateral relations. The Caucasian direction of Russian foreign policy is of great importance in questions of ensuring Russian national security. The Caucasian crisis of2008 showed potential conflictness of the region and safety hazard of Russia. Now other processes proceed already in the region. Armenian intention to join the Customs union, the change of Georgian leadership and signing of the agreement on association with EU by Georgia start new mechanisms of interaction between the region countries on the one hand and Russia on the other hand. The relation format between region republics can be changed. The main purpose of the article is to show Russian role in questions of the South Caucasus regional security. The region has the conflict territories, from which the Nagorno-Karabakh Republic, the Abkhazian Republic and the South Ossetia Republic are distinguished especially. And Russia won't be able to keep aloof in case of escalation of the conflicts around above-mentioned subjects. The vital questions for the region are peace and stability. And Russia plays a key role in these questions.

  3. Southern Pine Based on Biorefinery Center

    Energy Technology Data Exchange (ETDEWEB)

    Ragauskas, Arthur J. [Georgia Inst. of Technology, Atlanta, GA (United States); Singh, Preet [Georgia Inst. of Technology, Atlanta, GA (United States)

    2013-12-20

    This program seeks to develop an integrated southern pine wood to biofuels/biomaterials processing facility on the Recipient’s campus, that will test advanced integrated wood processing technologies at the laboratory scale, including: The generation of the bioethanol from pines residues and hemicelluloses extracted from pine woodchips; The conversion of extracted woodchips to linerboard and bleach grade pulps; and the efficient conversion of pine residues, bark and kraft cooking liquor into a useful pyrolysis oil.

  4. Electron Microscopy Society of Southern Africa : proceedings

    International Nuclear Information System (INIS)

    Snyman, H.C.; Coetzee, J.; Coubrough, R.I.

    1987-01-01

    The proceedings of the 26th annual conference of the Electron Microscopy Society of Southern Africa are presented. Papers were presented on the following topics: techniques and instrumentation used in electron microscopy, and applications of electron microscopy in the life sciences, including applications in medicine, zoology, botany and microbiology. The use of electron microscopy in the physical sciences was also discussed. Separate abstracts were prepared for seven of the papers presented. The remaining papers were considered outside the subject scope of INIS

  5. Intensity of plant collecting in southern Africa

    Directory of Open Access Journals (Sweden)

    G. E. Gibbs Russell

    1984-12-01

    Full Text Available The intensity of plant collecting in southern Africa is mapped using records from the Pretoria National Herbarium Computerized Information System (PRECIS, For the entire area, over 85% of the quarter degree grid squares have fewer than 100 specimens recorded. Collecting intensities are compared for different countries, biomes and climatic zones. Future field work from the National Herbarium will be concentrated in areas most seriously under-collected.

  6. Parameterized and resolved Southern Ocean eddy compensation

    Science.gov (United States)

    Poulsen, Mads B.; Jochum, Markus; Nuterman, Roman

    2018-04-01

    The ability to parameterize Southern Ocean eddy effects in a forced coarse resolution ocean general circulation model is assessed. The transient model response to a suite of different Southern Ocean wind stress forcing perturbations is presented and compared to identical experiments performed with the same model in 0.1° eddy-resolving resolution. With forcing of present-day wind stress magnitude and a thickness diffusivity formulated in terms of the local stratification, it is shown that the Southern Ocean residual meridional overturning circulation in the two models is different in structure and magnitude. It is found that the difference in the upper overturning cell is primarily explained by an overly strong subsurface flow in the parameterized eddy-induced circulation while the difference in the lower cell is mainly ascribed to the mean-flow overturning. With a zonally constant decrease of the zonal wind stress by 50% we show that the absolute decrease in the overturning circulation is insensitive to model resolution, and that the meridional isopycnal slope is relaxed in both models. The agreement between the models is not reproduced by a 50% wind stress increase, where the high resolution overturning decreases by 20%, but increases by 100% in the coarse resolution model. It is demonstrated that this difference is explained by changes in surface buoyancy forcing due to a reduced Antarctic sea ice cover, which strongly modulate the overturning response and ocean stratification. We conclude that the parameterized eddies are able to mimic the transient response to altered wind stress in the high resolution model, but partly misrepresent the unperturbed Southern Ocean meridional overturning circulation and associated heat transports.

  7. [The new migratory deal in Southern Europe].

    Science.gov (United States)

    Simon, G

    1986-09-01

    The author examines migration patterns in Southern Europe during the 1970s and early 1980s, noting particularly the reduction in migration northward from this region. It is noted that "departure potential remains sizable in certain areas of Portugal, Spain, Southern Italy, and most particularly, of Turkey and Yugoslavia. Transoceanic migrations have by no means ceased, as new flows of skilled labor have, since 1974, gone towards Arab states endowed with petrol (oil) revenues. And yet, the paramount fact is most surely the emergence and the proliferation in Greece, Spain, and (especially) in Italy, of basically clandestine (illegal) immigration. This movement is due to the convergence of several factors: economic and demographic disparities between northern and southern shores of the Mediterranean Sea, the sealing-off of borders in Northwestern Europe and the 'carry-over' effect upon nations of 'transit', the extent of the flow of refugees, and--most particularly--the appeal provided by the development, in these new employer countries, of an underground economy accompanied by the extension into industry of the practice of 'undeclared' work. And notwithstanding the series of rules lastly drawn up in Spain and in Greece, such forms of clandestine (unauthorized) migration appear highly likely--to say the least--to persist." (SUMMARY IN ENG AND SPA) excerpt

  8. Water. Unresolved emergency of the Southern hemisphere

    International Nuclear Information System (INIS)

    Lotti, C.

    2001-01-01

    Among the serious dramas in the Southern hemisphere, the most serious relates to health, and the most serious health problem is dysentery among the rural population, caused by the lack of unpolluted water. Bad management frustrates much of the aid sent to this part of the world, including projects to help the water crisis. In view of the success, it would appear that a concrete solution could be found within a decade, at a cost that can easily be sustained by the rich world, so long as funds are managed honestly. Much more ambitious and, at least for the moment, beyond all concrete possibility, is the project to provide a barrel of water per head to each person in the southern hemisphere. Providing unpolluted water and facilitating farming development through minor irrigation and family cattle-raising schemes is an idea that appears entirely Utopian. The conclusion provides a critical analysis of the major hydraulic works essential for true development, but effective only if the human environment is ready to receive them, whereas minor works are useful in any case - at village level - to resolve the thirst of the southern hemisphere [it

  9. Early German plans for southern observatories

    Science.gov (United States)

    Wolfschmidt, G.

    2002-07-01

    As early as the 18th and 19th centuries, French and English observers were active in South Africa. Around the beginning of the 20th century, Heidelberg and Potsdam astronomers proposed a southern observatory. Then Göttingen astronomers suggested building an observatory in Windhoek for photographing the sky and measuring the solar constant. In 1910 Karl Schwarzschild (1873-1916), after a visit to observatories in the United States, pointed out the usefulness of an observatory in South West Africa, in a climate superior to that in Germany, giving German astronomers access to the southern sky. Seeing tests were begun in 1910 by Potsdam astronomers, but WW I stopped the plans. In 1928 Erwin Finlay-Freundlich (1885-1964), inspired by the Hamburg astronomer Walter Baade (1893-1960), worked out a detailed plan for a southern observatory with a reflecting telescope, spectrographs and an astrograph with an objective prism. Paul Guthnick (1879-1947), director of the Berlin observatory, in cooperation with APO Potsdam and Hamburg, made a site survey to Africa in 1929 and found the conditions in Windhoek to be ideal. Observations were started in the 1930s by Berlin and Breslau astronomers, but were stopped by WW II. In the 1950s, astronomers from Hamburg and The Netherlands renewed the discussion in the framework of European cooperation, and this led to the founding of ESO in 1963.

  10. Towards Marine Spatial Planning in Southern Taiwan

    Directory of Open Access Journals (Sweden)

    Meng-Tsung Lee

    2014-11-01

    Full Text Available Due to population growth, rapid economic development and inadequate marine control, the use of ocean and coastal regions in Taiwan has become more frequent and intense in recent years. However, the lack of comprehensive marine and coastal planning in this island nation has led to many conflicts over space and resources and limited its ability to prepare for and respond to environmental hazards, thus threatening national security as well as the safety and property of its citizens. This study proposes a marine zoning scheme for southern Taiwan. The results show that many important habitats in the southern sea areas have not been properly protected due to the extremely small size of the marine protected area. Furthermore, the majority of the conflicts derive from the exclusive fishing right vs. other uses such as marine conservation. Therefore, it is crucial to establish the marine spatial planning (MSP for the Southern Taiwan to deal with the conflicts of use seas and uncertainties associated with complex, heterogeneous, and dynamic marine system.

  11. Industrial Physics---Southern California Style

    Science.gov (United States)

    Leslie, Stuart

    2013-03-01

    Only in Southern California did space-age style really come into its own as a unique expression of Cold War scientific culture. The corporate campuses of General Atomic in San Diego and North American Aviation in Los Angeles perfectly expressed the exhilarating spirit of Southern California's aerospace era, scaling up the residential version of California modernism to industrial proportion. Architects William Pereira and A.C. Martin Jr., in collaboration with their scientific counterparts, fashioned military-industrial `dream factories' for industrial physics that embodied the secret side of the space-age zeitgeist, one the public could only glimpse of in photographs, advertisements, and carefully staged open houses. These laboratories served up archetypes of the California dream for a select audience of scientists, engineers, and military officers, live-action commercials for a lifestyle intended to lure the best and brightest to Southern California. Paradoxically, they hid in plain sight, in the midst of aerospace suburbs, an open secret, at once visible and opaque, the public face of an otherwise invisible empire. Now, at the end of the aerospace era, these places have become an endangered species, difficult to repurpose, on valuable if sometimes highly polluted land. Yet they offer an important reminder of a more confident time when many physicists set their sights on the stars.

  12. Industry Based Survey (IBS) Yellowtail

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The "Southern New England Yellowtail Flounder Industry-Based Survey" was a collaboration between the Rhode Island Division of Fish and Wildlife and the fishing...

  13. Ecology of southern ocean pack ice.

    Science.gov (United States)

    Brierley, Andrew S; Thomas, David N

    2002-01-01

    Around Antarctica the annual five-fold growth and decay of sea ice is the most prominent physical process and has a profound impact on marine life there. In winter the pack ice canopy extends to cover almost 20 million square kilometres--some 8% of the southern hemisphere and an area larger than the Antarctic continent itself (13.2 million square kilometres)--and is one of the largest, most dynamic ecosystems on earth. Biological activity is associated with all physical components of the sea-ice system: the sea-ice surface; the internal sea-ice matrix and brine channel system; the underside of sea ice and the waters in the vicinity of sea ice that are modified by the presence of sea ice. Microbial and microalgal communities proliferate on and within sea ice and are grazed by a wide range of proto- and macrozooplankton that inhabit the sea ice in large concentrations. Grazing organisms also exploit biogenic material released from the sea ice at ice break-up or melt. Although rates of primary production in the underlying water column are often low because of shading by sea-ice cover, sea ice itself forms a substratum that provides standing stocks of bacteria, algae and grazers significantly higher than those in ice-free areas. Decay of sea ice in summer releases particulate and dissolved organic matter to the water column, playing a major role in biogeochemical cycling as well as seeding water column phytoplankton blooms. Numerous zooplankton species graze sea-ice algae, benefiting additionally because the overlying sea-ice ceiling provides a refuge from surface predators. Sea ice is an important nursery habitat for Antarctic krill, the pivotal species in the Southern Ocean marine ecosystem. Some deep-water fish migrate to shallow depths beneath sea ice to exploit the elevated concentrations of some zooplankton there. The increased secondary production associated with pack ice and the sea-ice edge is exploited by many higher predators, with seals, seabirds and whales

  14. Southern Hemisphere Upper Thermospheric Wind Climatology

    Science.gov (United States)

    Dhadly, M. S.; Emmert, J. T.; Drob, D. P.

    2017-12-01

    This study is focused on the poorly understood large-scale upper thermospheric wind dynamics in the southern polar cap, auroral, and mid latitudes. The gaps in our understanding of the dynamic high-latitude thermosphere are largely due to the sparseness of thermospheric wind measurements. Using data from current observational facilities, it is unfeasible to construct a synoptic picture of the Southern Hemisphere upper thermospheric winds. However, enough data with wide spatial and temporal coverage have accumulated to construct a meaningful statistical analysis of winds as function of season, magnetic latitude, and magnetic local time. We use long-term data from nine ground-based stations located at different southern high latitudes and three space-based instruments. These diverse data sets possess different geometries and different spatial and solar coverage. The major challenge of the effort is to combine these disparate sources of data into a coherent picture while overcoming the sampling limitations and biases among the datasets. Our preliminary analyses show mutual biases present among some of them. We first address the biases among various data sets and then combine them in a coherent way to construct maps of neutral winds for various seasons. We then validate the fitted climatology against the observational data and compare with corresponding fits of 25 years of simulated winds from the National Center for Atmospheric Research Thermosphere-Ionosphere-Electrodynamics General Circulation Model. This study provides critical insight into magnetosphere-ionosphere-thermosphere coupling and sets a necessary benchmark for validating new observations and tuning first-principles models.

  15. Holocene glacial fluctuations in southern South America

    Science.gov (United States)

    Reynhout, S.; Sagredo, E. A.; Kaplan, M. R.; Aravena, J. C.; Martini, M. A.; Strelin, J. A.; Schaefer, J. M.

    2016-12-01

    Understanding the timing and magnitude of former glacier fluctuations is critical to decipher long-term climatic trends and to unravel both natural cycles and human impact on the current glacial behavior. Despite more than seven decades of research efforts, a unifying model of Holocene glacial fluctuations in Southern South America remains elusive. Here, we present the state-of-the-art regarding the timing of Holocene glacial fluctuation in southern Patagonia-Tierra del Fuego, with a focus on a new generation of high-resolution radiocarbon and 10Be surface exposure dating chronologies. Recently acquired evidence suggest that after receding from advanced Late Glacial positions, Patagonian glaciers were for the most part close to, or even behind, present ice margins during the Early Holocene. On the other hand, emerging chronologies indicate that in some areas there were extensive expansions (century scale?) that punctuated the warm interval. Subsequently, we have evidence of multiple millennial timescale glacial advances starting in the middle Holocene. Several glacial maxima are defined by moraines and other landforms from 7000 years ago to the 19th century, with a gap sometime between 4,500 and 2,500 years ago. The last set of advances began around 800-600 years ago. Although glacial activity is documented in Patagonia at the same time as the European Little Ice Age, the extent of these glacial events are less prominent than those of the mid-Holocene. The causes that may explain these glacial fluctuations remain elusive. Finally, we discuss ongoing efforts to better define the timing and extent of Holocene glaciations in southern South America, and to establish the basis to test competing hypothesis of regional Holocene climate variability.

  16. Building the Southern California Earthquake Center

    Science.gov (United States)

    Jordan, T. H.; Henyey, T.; McRaney, J. K.

    2004-12-01

    Kei Aki was the founding director of the Southern California Earthquake Center (SCEC), a multi-institutional collaboration formed in 1991 as a Science and Technology Center (STC) under the National Science Foundation (NSF) and the U. S. Geological Survey (USGS). Aki and his colleagues articulated a system-level vision for the Center: investigations by disciplinary working groups would be woven together into a "Master Model" for Southern California. In this presentation, we will outline how the Master-Model concept has evolved and how SCEC's structure has adapted to meet scientific challenges of system-level earthquake science. In its first decade, SCEC conducted two regional imaging experiments (LARSE I & II); published the "Phase-N" reports on (1) the Landers earthquake, (2) a new earthquake rupture forecast for Southern California, and (3) new models for seismic attenuation and site effects; it developed two prototype "Community Models" (the Crustal Motion Map and Community Velocity Model) and, perhaps most important, sustained a long-term, multi-institutional, interdisciplinary collaboration. The latter fostered pioneering numerical simulations of earthquake ruptures, fault interactions, and wave propagation. These accomplishments provided the impetus for a successful proposal in 2000 to reestablish SCEC as a "stand alone" center under NSF/USGS auspices. SCEC remains consistent with the founders' vision: it continues to advance seismic hazard analysis through a system-level synthesis that is based on community models and an ever expanding array of information technology. SCEC now represents a fully articulated "collaboratory" for earthquake science, and many of its features are extensible to other active-fault systems and other system-level collaborations. We will discuss the implications of the SCEC experience for EarthScope, the USGS's program in seismic hazard analysis, NSF's nascent Cyberinfrastructure Initiative, and other large collaboratory programs.

  17. Southern Ocean Convection and tropical telleconnections

    Science.gov (United States)

    Marinov, I.; Cabre, A.; Gnanadesikan, A.

    2014-12-01

    We show that Southern Ocean (SO) temperatures in the latest generation of Earth System Models exhibit two major modes of variation, one driven by deep convection, the other by tropical variability. We perform a CMIP5 model intercomparison to understand why different climate models represent SO variability so differently in long, control simulations. We show that multiyear variability in Southern Ocean sea surface temperatures (SSTs) can in turn influence oceanic and atmospheric conditions in the tropics on short (atmospheric) time-scales. We argue that the strength and pattern of SO-tropical teleconnections depends on the intensity of SO deep convection. Periodic convection in the SO is a feature of most CMIP5 models under preindustrial forcing (deLavergne et al., 2014). Models show a wide distribution in the spatial extent, periodicity and intensity of their SO convection, with some models convecting most of the time, and some showing very little convection. In a highly convective coupled model, we find that multidecadal variability in SO and global SSTs, as well as SO heat storage are driven by Weddell Sea convective variability, with convective decades relatively warm due to the heat released from the deep southern ocean and non-convective decades cold due to the subsurface storage of heat. Furthermore, pulses of SO convection drive SST and sea ice variations, influencing absorbed shortwave and emitted longwave radiation, wind, cloud and precipitation patterns, with climatic implications for the low latitudes via fast atmospheric teleconnections. We suggest that these high-low latitude teleconnection mechanisms are relevant for understanding hiatus decades. Additionally, Southern Ocean deep convection varied significantly during past, natural climate changes such as during the last deglaciation. Weddell Sea open convection was recently weakened, likely as a consequence of anthropogenic forcing and the resulting surface freshening. Our study opens up the

  18. The Herpetology of the Southern Kalahari domain

    Directory of Open Access Journals (Sweden)

    W. D Haacke

    1984-12-01

    Full Text Available The herpetofauna of the southern Kalahari has mixed affinities, as this area lies on a rainfall gradient in a critical area where a transition between the arid south-west and the moister north-east takes place. As the variation in substrate type is relatively limited, the effect of the rainfall gradient appears to influence and determine the range limits of many taxa in both directions, resulting in an area in which of 55 recorded reptiles, 11 western taxa overlap or form a parapatric zone with 25 eastern taxa, while the remaining taxa are endemic or wideranging.

  19. The Southern Ocean and South Pacific Region

    OpenAIRE

    Kelleher, K.; Warnau, Michel; Failler, Pierre; Pecl, Gretta; Turley, Carol; Boeuf, Gilles; Laffoley, Dan; Parker, Laura; Gurney, Leigh

    2012-01-01

    The Region comprises three sub-regions (FAO Statistical Areas) with very different characteristics. The South Pacific includes the vast and virtually unpopulated Southern Ocean surrounding the Antarctic. It has the world’s largest fisheries off Peru and Chile and some of the world’s best managed fisheries in Australia and New Zealand. The Region has over 27% of the world’s ocean area and over 98% of the Region’s total area of 91 million km2 is ‘open ocean’. The Region contains less than 5% of...

  20. Epidemiology of cerebral palsy in Southern Denmark

    DEFF Research Database (Denmark)

    Frøslev-Friis, Christina; Dunkhase-Heinl, Ulrike; Andersen, Johnny Dohn Holmgren

    2015-01-01

    INTRODUCTION: The aim of this study was to describe the prevalence, subtypes, severity and neuroimaging findings of cerebral palsy (CP) in a cohort of children born in Southern Denmark. Risk factors were analysed and aetiology considered. METHODS: A population-based cohort study covering 17...... prevention of CP is possible if the numbers of preterm births and multiple pregnancies can be reduced. FUNDING: The Danish Cerebral Palsy Follow-up Programme is supported by the foundation "Ludvig og Sara Elsass Fond". TRIAL REGISTRATION: 2008-58-0034....

  1. Uranium mineralization of migmatite in southern China

    Energy Technology Data Exchange (ETDEWEB)

    Feng, Mingyue

    1987-09-01

    The uranium mineralization occurs in migmatite in the form of disseminated uraninite is a new type found in southern China. According to the forms of uraninite existence in orebodies, it can be divided into primary and reworked subtypes. The principal uranium mineral in the deposits of primary subtype is uraninite, but those in reworked subtype are pitchblende and relict uraninite. The formation of uranium mineralization is considered as a result of remobilization, migration and local concentration caused by preferential melting of the uranium-rich strata.

  2. Uranium mineralization of migmatite in southern China

    International Nuclear Information System (INIS)

    Feng Mingyue.

    1987-01-01

    The uranium mineralization occurs in migmatite in the form of disseminated uraninite is a new type found in southern China. According to the forms of uraninite existence in orebodies, it can be divided into primary and reworked subtypes. The principal uranium mineral in the deposits of primary subtype is uraninite, but those in reworked subtype are pitchblende and relict uraninite. The formation of uranium mineralization is considered as a result of remobilization, migration and local concentration caused by preferential melting of the uranium-rich strata

  3. Stroemgren photometry of southern white dwarfs

    International Nuclear Information System (INIS)

    Bessell, M.S.; Wickramasinghe, D.T.

    1978-01-01

    Colours of southern white dwarfs in the uvby (Stroemgren four-colour) system have been obtained. The results are compared with those of Graham. The extensive absolute photometry of white dwarfs published by Greenstein has also been transferred into the four-colour system and both sets of results are compared with model atmosphere calculations. The scatter in log (g) is higher than previously supposed, and the evidence for an increase in at the cooler (Tsub(e) < 10 000 K) end of the DA sequence is discussed. (author)

  4. Dryland orchard development in southern Tamil Nadu

    International Nuclear Information System (INIS)

    Mohandas, S.; Marimuthu, R.; Karthikeyan, M.

    2002-01-01

    With low annual rainfall and high potential of evaporation in southern districts of Tamil Nadu, it is difficult to grow the crops, successfully. Drylands of this tract are vulnerable to high radiation, high temperature and variable rainfall. Hence, dryfarming has become a risky enterprise in this zone and all the above factors altogether reduce the choice of agricultural crops. Considering the magnitude of the area and it's untapped potential, which offers enormous scope for dryland horticulture in this region and thereby improving the soil productivity for other crops too

  5. H2 fuelling infrastructure in Southern California

    International Nuclear Information System (INIS)

    Miyasato, M.

    2004-01-01

    'Full text:' The South Coast Air Quality Management District (SCAQMD) is the local air agency covering the majority of southern California, and the entity legislatively responsible for bringing the region into compliance with the federal Clean Air standards by 2010. One of the tools used by the SCAQMD to accelerate achieving cleaner air is the funding of research, development, and demonstration projects for advanced, clean air technologies. One major focus has been on hydrogen and fuel cells for both stationary and mobile applications. The presentation will discuss the SCAQMD strategy and deployment efforts regarding the development and expansion of hydrogen fueling infrastructure in the South Coast Air Basin. (author)

  6. Automated design of genomic Southern blot probes

    Directory of Open Access Journals (Sweden)

    Komiyama Noboru H

    2010-01-01

    Full Text Available Abstract Background Sothern blotting is a DNA analysis technique that has found widespread application in molecular biology. It has been used for gene discovery and mapping and has diagnostic and forensic applications, including mutation detection in patient samples and DNA fingerprinting in criminal investigations. Southern blotting has been employed as the definitive method for detecting transgene integration, and successful homologous recombination in gene targeting experiments. The technique employs a labeled DNA probe to detect a specific DNA sequence in a complex DNA sample that has been separated by restriction-digest and gel electrophoresis. Critically for the technique to succeed the probe must be unique to the target locus so as not to cross-hybridize to other endogenous DNA within the sample. Investigators routinely employ a manual approach to probe design. A genome browser is used to extract DNA sequence from the locus of interest, which is searched against the target genome using a BLAST-like tool. Ideally a single perfect match is obtained to the target, with little cross-reactivity caused by homologous DNA sequence present in the genome and/or repetitive and low-complexity elements in the candidate probe. This is a labor intensive process often requiring several attempts to find a suitable probe for laboratory testing. Results We have written an informatic pipeline to automatically design genomic Sothern blot probes that specifically attempts to optimize the resultant probe, employing a brute-force strategy of generating many candidate probes of acceptable length in the user-specified design window, searching all against the target genome, then scoring and ranking the candidates by uniqueness and repetitive DNA element content. Using these in silico measures we can automatically design probes that we predict to perform as well, or better, than our previous manual designs, while considerably reducing design time. We went on to

  7. Laser ablation studies in southern Africa

    Science.gov (United States)

    McKenzie, Edric; Forbes, A.; Turner, G. R.; Michaelis, Max M.

    2000-08-01

    With the launch of the South African National Laser Centre, new programs will need to be defined. Medical, environmental and industrial laser applications must obviously take top priority -- as opposed to the uranium isotope separation and military applications of the past. We argue however, that a small effort in laser ablation for space propulsion is justifiable, since a few very large CO2 lasers are available and since two tentative propulsion experiments have already been conducted in South Africa. We attempt to give LISP (Laser Impulse Space Propulsion) an equatorial and a Southern dimension.

  8. Anthropogenic radiative forcing of southern African and Southern Hemisphere climate variability and change

    CSIR Research Space (South Africa)

    Engelbrecht, FA

    2014-10-01

    Full Text Available of stratospheric ozone, greenhouse gasses, aerosols and sulphur dioxide, can improve the model's skill to simulate inter-annual variability over southern Africa. The paper secondly explores the role of different radiative forcings of future climate change over...

  9. Zonally asymmetric response of the Southern Ocean mixed-layer depth to the Southern Annular Mode

    Science.gov (United States)

    Sallée, J. B.; Speer, K. G.; Rintoul, S. R.

    2010-04-01

    Interactions between the atmosphere and ocean are mediated by the mixed layer at the ocean surface. The depth of this layer is determined by wind forcing and heating from the atmosphere. Variations in mixed-layer depth affect the rate of exchange between the atmosphere and deeper ocean, the capacity of the ocean to store heat and carbon and the availability of light and nutrients to support the growth of phytoplankton. However, the response of the Southern Ocean mixed layer to changes in the atmosphere is not well known. Here we analyse temperature and salinity data from Argo profiling floats to show that the Southern Annular Mode (SAM), the dominant mode of atmospheric variability in the Southern Hemisphere, leads to large-scale anomalies in mixed-layer depth that are zonally asymmetric. From a simple heat budget of the mixed layer we conclude that meridional winds associated with departures of the SAM from zonal symmetry cause anomalies in heat flux that can, in turn, explain the observed changes of mixed-layer depth and sea surface temperature. Our results suggest that changes in the SAM, including recent and projected trends attributed to human activity, drive variations in Southern Ocean mixed-layer depth, with consequences for air-sea exchange, ocean sequestration of heat and carbon, and biological productivity.

  10. Southern Annular Mode drives multicentury wildfire activity in southern South America.

    Science.gov (United States)

    Holz, Andrés; Paritsis, Juan; Mundo, Ignacio A; Veblen, Thomas T; Kitzberger, Thomas; Williamson, Grant J; Aráoz, Ezequiel; Bustos-Schindler, Carlos; González, Mauro E; Grau, H Ricardo; Quezada, Juan M

    2017-09-05

    The Southern Annular Mode (SAM) is the main driver of climate variability at mid to high latitudes in the Southern Hemisphere, affecting wildfire activity, which in turn pollutes the air and contributes to human health problems and mortality, and potentially provides strong feedback to the climate system through emissions and land cover changes. Here we report the largest Southern Hemisphere network of annually resolved tree ring fire histories, consisting of 1,767 fire-scarred trees from 97 sites (from 22 °S to 54 °S) in southern South America (SAS), to quantify the coupling of SAM and regional wildfire variability using recently created multicentury proxy indices of SAM for the years 1531-2010 AD. We show that at interannual time scales, as well as at multidecadal time scales across 37-54 °S, latitudinal gradient elevated wildfire activity is synchronous with positive phases of the SAM over the years 1665-1995. Positive phases of the SAM are associated primarily with warm conditions in these biomass-rich forests, in which widespread fire activity depends on fuel desiccation. Climate modeling studies indicate that greenhouse gases will force SAM into its positive phase even if stratospheric ozone returns to normal levels, so that climate conditions conducive to widespread fire activity in SAS will continue throughout the 21st century.

  11. GPM Ground Validation Southern Appalachian Rain Gauge IPHEx V1

    Data.gov (United States)

    National Aeronautics and Space Administration — The GPM Ground Validation Southern Appalachian Rain Gauge IPHEx dataset was collected during the Integrated Precipitation and Hydrology Experiment (IPHEx) field...

  12. Climate change induced by Southern Hemisphere desertification

    Science.gov (United States)

    Wang, Ye; Yan, Xiaodong

    2017-12-01

    Some 10-20% of global dry-lands are already degraded, and the ongoing desertification threatens the world's poorest populations. Studies on desertification effects are essential for humans to adapt to the environmental challenges posed by desertification. Given the importance of the much larger southern ocean to the global climate and the Southern Hemisphere (SH) climate changes in phase with those in the north, the biogeophysical effects of the SH desertification on climate are assessed using an Earth system model of intermediate complexity, MPM-2. This analysis focuses on differences in climate among the averages of simulations with desert expansion in different latitude bands by year 2000. The localized desertification causes significant global changes in temperature and precipitation as well as surface albedo. On the global scale, cooling dominates the SH desertification effects. However, the biogeophysical effects are most significant in regions with desertification, and the cooling is also prominent in northern mid-latitudes. Desert expansion in 15°-30°S reveals statistically most significant cooling and increased precipitation over the forcing regions during spring. The global and regional scale responses from desertification imply the climate teleconnection and address the importance of the effects from the SH which are contingent on the location of the forcing. Our study indicates that biogeophysical mechanisms of land cover changes in the SH need to be accounted for in the assessment of land management options especially for latitude band over 15°-30°S.

  13. SOAR + SMARTS Southern White Dwarf Survey

    Science.gov (United States)

    Subasavage, John P.; Lepine, S.

    2012-01-01

    We present early results from the SOAR + SMARTS Southern White Dwarf SURVEY (SSSWDS). Our initial sift of relatively bright (15 color relation of Oppenheimer et al. 2001 are obtained and permit prioritized follow-up. For confirmation of luminosity class, we use the SOAR telescope atop Cerro Pachon equipped with the Goodman Spectrograph and a moderate resolution grating. In tandem, we acquire multi-epoch, optical Johnson-Kron-Cousins BVRI photometry using the SMARTS 1.0m telescope atop CTIO. Combined with JHK from 2MASS, we compare the photometric SED to relevant white dwarf model atmospheres to estimate physical parameters (e.g., effective temperature, mass) and distance. For the nearest targets, specifically those within the RECONS (www.recons.org) horizon of 25 pc, we aim to obtain trigonometric parallaxes as part of the Cerro Tololo Inter-American Observatory Parallax Investigation (CTIOPI) project being conducted at the SMARTS 0.9m telescope. To date, we have confirmed 100 relatively bright, new white dwarfs in the southern hemisphere. Of those, 13 are estimated to be within our 25 pc horizon-of-interest, including two that are estimated to be within 15 pc. Ongoing observations will boost these figures by the end of the project.

  14. Cooling tower water ozonation at Southern University

    International Nuclear Information System (INIS)

    Chen, C.C.; Knecht, A.T.; Trahan, D.B.; Yaghi, H.M.; Jackson, G.H.; Coppenger, G.D.

    1990-01-01

    Cooling-tower water is a critical utility for many industries. In the past, inexpensive water coupled with moderate regulation of discharge water led to the neglect of the cooling tower as an energy resource. Now, with the increased cost of chemical treatment and tough EPA rules and regulations, this situation is rapidly changing. The operator of the DOE Y-12 Plant in Oak Ridge as well as many other industries are forced to develop an alternate method of water treatment. The cooling tower is one of the major elements in large energy systems. The savings accrued from a well engineered cooling tower can be a significant part of the overall energy conservation plan. During a short-term ozonation study between 1987-1988, the Y-12 Plant has been successful in eliminating the need for cooling tower treatment chemicals. However, the long-term impact was not available. Since April 1988, the ozone cooling water treatment study at the Y-12 Plant has been moved to the site at Southern University in Baton Rouge, Louisiana. The purpose of this continued study is to determine whether the use of ozonation on cooling towers is practical from an economic, technical and environmental standpoint. This paper discusses system design, operating parameter and performance testing of the ozonation system at Southern University

  15. Plutonium in Southern Hemisphere Ocean Waters

    Energy Technology Data Exchange (ETDEWEB)

    Hirose, K. [Sophia University, Tokyo (Japan); Aoyama, M. [Meteorological Research Institute, Tsukuba (Japan); Gastaud, J.; Levy, I. [Marine Environment Laboratories, International Atomic Energy Agency (Monaco); Fukasawa, M. [Japan Agency for Marine-Earth Science and Technology Yokosuka (Japan); Kim, C. -S. [Environment Laboratories, International Atomic Energy Agency, Seibersdorf (Austria); Povinec, P. P. [Comenius University, Bratislava (Slovakia); Roos, P. [Riso National Laboratory, Roskilde (Denmark); Sanchez-Cabeza, J. A. [Universitat Autonoma de Barcelona, Bellaterra (Spain); Yim, S. A. [Korea Institute of Nuclear Safety, Daejeon (Korea, Republic of)

    2013-07-15

    Plutonium in seawater collected by the BEAGLE2003 cruise was determined using ICP-SF-MS and alpha spectrometry after Fe co-precipitation and radiochemical purification. Levels and distributions of dissolved plutonium activity concentrations in Southern Hemisphere ocean waters are summarized here, including historical data. Pu-239 concentrations in surface water of the central South Pacific (32.5{sup o}S) in 2003 were around 1 mBq/m{sup 3}. The {sup 239}Pu concentrations in the Indian Ocean surface waters (20{sup o}S) were similar to that in the South Pacific, whereas the {sup 239}Pu concentrations in the South Atlantic surface waters (30{sup o}S) were markedly lower than those in the South Pacific and Indian Oceans. The {sup 239}Pu vertical profile pattern was similar to that in the North Pacific subtropical gyre, although {sup 239}Pu concentrations in the deep South Pacific were significantly lower than those in the North Pacific. One of the dominant factors controlling plutonium distributions in the Southern Hemisphere oceans is biogeochemical processes including particle scavenging. (author)

  16. QUATERNARY HISTORY OF CEDRUS IN SOUTHERN EUROPE

    Directory of Open Access Journals (Sweden)

    D. Magri

    2012-05-01

    Full Text Available A database of 68 pollen records of Pleistocene age was compiled from the western Mediterranean regions, with the aim of reconstructing the history of Cedrus in south Europe during the last 2 Ma. Marine pollen records from the Alboran Sea suggest that Cedrus was present in Morocco throughout the Quaternary, while it was absent from the Iberian peninsula, except a possible local presence in a coastal site of southern Spain. In France, Cedrus pollen was recorded in Pliocene deposits, but its Quaternary finds are always very sparse and suggest a long distance origin of cedar pollen. Cedrus was widespread in all the Italian sites during the Early Pleistocene, but it is sporadically found in the Middle Pleistocene deposits. Although times and modes of the disappearance of Cedrus from Italy are not known, it appears that the marked climate changes occurred between 0.9 and 0.7 Ma determined its local extinction. A similar trend is found in Greece, where Cedrus may have persisted a little longer than in the Italian Peninsula. On the whole, the history of Cedrus in southern Europe indicates that it is a taxon vulnerable to global climate changes and warns of a future risk of extinction also in the rest of the Mediterranean Basin.

  17. Anthropocene Survival of Southern New England's Salt ...

    Science.gov (United States)

    In southern New England, salt marshes are exceptionally vulnerable to the impacts of accelerated sea level rise. Regional rates of sea level rise have been as much as 50 % greater than the global average over past decades, a more than fourfold increase over late Holocene background values. In addition, coastal development blocks many potential marsh migration routes, and compensatory mechanisms relying on positive feedbacks between inundation and sediment deposition are insufficient to counter inundation increases in extreme low-turbidity tidal waters. Accordingly, multiple lines of evidence suggest that marsh submergence is occurring in southern New England. A combination of monitoring data, field re-surveys, radiometric dating, and analysis of peat composition have established that, beginning in the early and mid-twentieth century, the dominant low-marsh plant, Spartina alterniflora, has encroached upward in tidal marshes, and typical high-marsh plants, including Juncus gerardii and Spartina patens, have declined, providing strong evidence that vegetation changes are being driven, at least in part, by higher water levels. Additionally, aerial and satellite imagery show shoreline retreat, widening and headward extension of channels, and new and expanded interior depressions. Papers in this special section highlight changes in marsh-building processes, patterns of vegetation loss, and shifts in species composition. The final papers turn to strategies for minimiz

  18. Reappraisal of the seismotectonics of southern Ontario

    International Nuclear Information System (INIS)

    Mohajer, A.A.

    1987-11-01

    The fundamental objectives of this study were to review and improve the seismological data base as an aid in more realistic evaluation of seismic hazard in southern Ontario. For this purpose, the following procedures have been undertaken: In the first stage, the types of errors in earthquake location files are identified, sources of uncertainties are discussed and a sensitivity analysis of the errors to different parameters is presented. In the second stage, a group location technique, Joint Hypocenter Determination (JHD), has been utilized to improve the locations of a group of 67 well-recorded events, mostly from the more active region near the Ottawa River and in western Quebec. The third stage, to relocate smaller and less reliably detected events in southern Ontario, utilized a nw algorithm, 'HYPOCENTER', which proved very efficient and flexible in the test runs for handling local explosion and natural events. A preliminary interpretation of the seismicity patterns in the study regions shows that earthquakes of magnitude 3 and larger tend to align along preferred seismic trends which may, in turn, be controlled by weakness planes in the Earth's crust. These inferred trends coincide with dominant northwesterly and northeasterly striking structural directions. For earthquakes smaller than magnitude 4 prior to 1970 and for microearthquakes (M<3) which occurred near the Lake Ontario shoreline, the detection coverage was not sufficient to conclusively discuss accurate locations and causative mechanisms

  19. Biological Correlates of Northern-Southern Italy Differences in IQ

    Science.gov (United States)

    Templer, Donald I.

    2012-01-01

    The present study was intended to provide perspective, albeit less than unequivocal, on the research of Lynn (2010) who reported higher IQs in the northern than southern Italian regions. He attributes this to northern Italians having a greater genetic similarity to middle Europeans and southern Italians to Mediterranean people. Higher regional IQ…

  20. Interest group opinions about fuel reduction in southern Appalachia

    Science.gov (United States)

    Carin E. Vadala; Robert D. Bixler; Thomas A. Waldrop

    2013-01-01

    Opinions of interested publics and interest groups (n = 640) about fuel reduction (FR) in the Southern Appalachian Mountains were investigated through social survey using both pictorial and written questions. The study identified three discrete groups based on knowledge of forest history in the Southern Appalachian Mountains, attitudes toward social and ecological...

  1. The earliest unequivocally modern humans in southern China

    NARCIS (Netherlands)

    Liu, Wu; Martinón-Torres, María; Cai, Yan-Jun; Xing, Song; Tong, Hao-Wen; Pei, Shu-Wen; Sier, Mark Jan; Wu, Xiao-Hong; Edwards, R Lawrence; Cheng, Hai; Li, Yi-Yuan; Yang, Xiong-Xin; de Castro, José María Bermúdez; Wu, Xiu-Jie

    2015-01-01

    The hominin record from southern Asia for the early Late Pleistocene epoch is scarce. Well-dated and well-preserved fossils older than ∼45,000 years that can be unequivocally attributed to Homo sapiens are lacking. Here we present evidence from the newly excavated Fuyan Cave in Daoxian (southern

  2. Dust from southern Africa: rates of emission and biogeochemical properties

    Science.gov (United States)

    Bhattachan, A.; D'Odorico, P.; Zobeck, T. M.; Okin, G. S.; Dintwe, K.

    2012-12-01

    The stabilized linear dunefields in the southern Kalahari show signs of reactivation due to reduced vegetation cover owing to drought and/or overgrazing. It has been demonstrated with a laboratory dust generator that the southern Kalahari soils are good emitters of dust and that large-scale dune reactivation can potentially make the region an important dust source in the relatively low-dust Southern Hemisphere. We show that emergence of the southern Kalahari as a new dust source may affect ocean biogeochemistry as the soils are rich in soluble iron and the dust from the southern Kalahari commonly reaches the Southern Ocean. We investigate the biogeochemical properties of the fine fraction of soil from the Kalahari dunes and compare them to those of currently active dust sources such as the Makgadikgadi and the Etosha pans as well as other smaller pans in the region. Using field measurements of sediment fluxes and satellite images, we calculate the rates of dust emission from the southern Kalahari under different land cover scenarios. To assess the reversibility of dune reactivation in the southern Kalahari, we investigate the resilience of dunefield vegetation by looking at changes in soil nutrients, fine soil fractions, and seed bank in areas affected by intense denudation.

  3. Patterns of endemicity and range restriction among southern African ...

    African Journals Online (AJOL)

    Patterns of endemicity and range restriction among southern African coastal marine invertebrates. RJ Scott, CL Griffiths, TB Robinson. Abstract. Southern Africa supports a rich marine biota of 12 734 currently described marine species. Although the distribution and overall species-richness patterns of several component ...

  4. Prevalence of diabetes mellitus in the rural southern Free State ...

    African Journals Online (AJOL)

    Prevalence of diabetes mellitus in the rural southern Free State. ... The objectives of this study were to determine the prevalence of DM in the rural southern Free State and to investigate the contribution of risk ... A need for intervention regarding the identification and treatment of DM in these rural areas has been identified.

  5. Approaching Southern Theory: Explorations of Gender in South African Education

    Science.gov (United States)

    Epstein, Debbie; Morrell, Robert

    2012-01-01

    This article draws on the five other papers from South Africa in this issue of "Gender and Education" to consider how Southern theory has been developed and is developing in relation to gender and education in South Africa. We argue that Southern theory is not an on-the-shelf solution to global geopolitical inequalities but a work in…

  6. Breeding density of the southern ground hornbill, Bucorvus ...

    African Journals Online (AJOL)

    The southern ground hornbill, Bucorvus leadbeateri, is on the IUCN Red List of Threatened Species as a 'Vulnerable' species with a decreasing population. Despite a myriad of threats, including loss of nesting sites, accidental poisoning and persecution, there has been no detailed study of the southern ground hornbill in ...

  7. A new tree classification system for southern hardwoods

    Science.gov (United States)

    James S. Meadows; Daniel A. Jr. Skojac

    2008-01-01

    A new tree classification system for southern hardwoods is described. The new system is based on the Putnam tree classification system, originally developed by Putnam et al., 1960, Management ond inventory of southern hardwoods, Agriculture Handbook 181, US For. Sew., Washington, DC, which consists of four tree classes: (1) preferred growing stock, (2) reserve growing...

  8. Minimizing and predicting delamination of southern plywood in exterior exposure

    Science.gov (United States)

    Peter Koch

    1967-01-01

    Southern pine plywood is substantially all being manufactured with phenolic glue for exterior use. Because panels must not delaminate in service, a reliable predictor of glueline durability is required. Drawing on the experience of the Douglas-fir plywood industry, southern manufacutrers have adopted as a predictor the percentage of wood failure(% WF) observed in...

  9. AIDS Prevention in the Southern African Development Community ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    AIDS Prevention in the Southern African Development Community : Policy Research and Decision Support. The Southern African Development Community (SADC) is at the epicentre of the AIDS pandemic. The regional adult HIV prevalence is approximately 11%, twice the average in other African countries. Scores of ...

  10. Book review: Southern Forested Wetlands: Ecology and Management

    Science.gov (United States)

    Carl C. Trettin

    2000-01-01

    The southern region has the largest proportion of wetlands in the conterminous US. The majority of that wetland resource is forested by diverse vegetation communities reflecting differences in soil, hydrology, geomorphology, climatic conditions and past management. Wetland resources in the southern US are very important to the economy providing both commodity and non-...

  11. Mercury bioaccumulation in Southern Appalachian birds, assessed through feather concentrations

    Science.gov (United States)

    Rebecca Hylton Keller; Lingtian Xie; David B. Buchwalter; Kathleen E. Franzreb; Theodore R Simons

    2014-01-01

    Mercury contamination in wildlife has rarely been studied in the Southern Appalachians despite high deposition rates in the region. From 2006 to 2008 we sampled feathers from 458 birds representing 32 species in the Southern Appalachians for total mercury and stable isotope ä 15N. Mercury concentrations (mean ± SE) averaged 0.46...

  12. Southern Research Station Global Change Research Strategy 2011-2019

    Science.gov (United States)

    Kier Klepzig; Zoe Hoyle; Stevin Westcott; Emrys Treasure

    2012-01-01

    In keeping with the goals of the Research and Development agenda of the Forest Service, U.S. Department of Agriculture (USDA), the Southern Research Station (SRS) provides the information and technology needed to develop best management practices for the forest lands of the Southern United States, where science-guided actions are needed to sustain ecosystem health,...

  13. Forest fires and air quality issues in southern Europe

    Science.gov (United States)

    Ana Isabel Miranda; Enrico Marchi; Marco Ferretti; Millán M. Millán

    2009-01-01

    Each summer forest fires in southern Europe emit large quantities of pollutants to the atmosphere. These fires can generate a number of air pollution episodes as measured by air quality monitoring networks. We analyzed the impact of forest fires on air quality of specific regions of southern Europe. Data from several summer seasons were studied with the aim of...

  14. Proceedings of the 23rd Southern Forest Tree Improvement Conference

    Science.gov (United States)

    Robert J. Weir; Alice V. Hatcher; [Compilers

    1995-01-01

    The 23rd Southern Forest Tree Improvement Conference was held at the Holiday Inn SunSpree Resort in Asheville, North Carolina. The Conference was sponsored by the Southern Forest Tree Improvement Committee and hosted by the N. C. State University-Industry Cooperative Tree Improvement Program. A total of 37 presentations, three invited and 34 voluntary, were given....

  15. 46 CFR 42.30-10 - Southern Winter Seasonal Zone.

    Science.gov (United States)

    2010-10-01

    ... Island; thence the rhumb line to Black Rock Point on Stewart Island; thence the rhumb line to the point... BY SEA Zones, Areas, and Seasonal Periods § 42.30-10 Southern Winter Seasonal Zone. (a) The northern boundary of the Southern Winter Seasonal Zone is the rhumb line from the east coast of the American...

  16. Crop model usefulness in drylands of southern Africa: an application ...

    African Journals Online (AJOL)

    Data limitations in southern Africa frequently hinder adequate assessment of crop models before application. ... three locations to represent varying cropping and physical conditions in southern Africa, i.e. maize and sorghum (Mohale's Hoek, Lesotho and Big Bend, Swaziland) and maize and groundnut (Lilongwe, Malawi).

  17. Storm wave deposits in southern Istria (Croatia)

    Science.gov (United States)

    Biolchi, Sara; Furlani, Stefano; Devoto, Stefano; Scicchitano, Giovanni

    2017-04-01

    The accumulation of large boulders related to extreme waves are well documented in different areas of the Mediterranean coasts, such as in Turkey, Algeria, Egypt, Greece (Lesbos and Crete islands), France, Spain, Malta, Italy (Sicily and Apulia regions). These deposits have been associated to storm or tsunami events or both, depending on the local history. If compared to the Mediterranean Sea, the Adriatic Sea is considered a shallow basin, with very low wave energy. In particular the NE Adriatic, where Istria Peninsula (Croatia) is located, geological and geomorphological evidences of extreme wave events have never been described, as well as no tsunamis have been registered. We present the boulder deposits that have been recently found out in southern Istria, at Premantura and Marlera localities and we discuss the mechanisms that could have been responsible of the detachment and movement of these large rocky blocks from the emerged part of the coast and from the sea bottom inland. A multidisciplinary approach was adopted: geological and geomorphological surveyings, UAV and digital photogrammetric analysis, applying of the hydrodynamic equations as well as underwater profiles were carried out between 2012 and 2016. The southern Istrian coasts are composed of Cretaceous bedded limestones, sub-horizontal or gently inclined toward the sea and are exposed to southern winds, Scirocco and Libeccio, with wide fetch. The boulder deposits occur in correspondence of flat promontories or ancient quarry pavements, where the topography, together with the bedding planes and a dense fracture pattern constitute the predisposing factors of the boulder sizing and detachment. Boulder sizes, density, position and elevation have been measured in order to apply the hydrodynamic equations, which provide wave height values that can discriminate a storm from a tsunami origin. Biogenic marine encrustations, sometimes very recent, have been observed on large part of the boulders, attesting

  18. Epilepsy care in the southern Caribbean.

    Science.gov (United States)

    Krauss, Gregory; Sandy, Sherry; Corbin, David O C; Bird-Compton, Jacqueline; Jack, Frances; Nelson, Beverly; Jalonen, Tuula O; Ali, Amza; Fortuné, Taryn; Clarke, Dave; Okolie, Jacqueline; Cervenka, Mackenzie C

    2015-10-01

    Very little has been reported about the health resources available for patients with epilepsy in the five English-speaking southern Caribbean countries of Trinidad and Tobago, Barbados, Grenada, Saint Vincent and the Grenadines, and Saint Lucia. There is no comprehensive resource describing their health systems, access to specialty care, antiepileptic drug (AED) use, and availability of brain imaging and EEG. The purpose of this study was to profile epilepsy care in these countries as an initial step toward improving the standard of care and identifying gaps in care to guide future policy changes. In each southern Caribbean country, we conducted study visits and interviewed health-care providers, government health ministers, pharmacy directors, hospital medical directors, pharmacists, clinic staff, radiologists, and radiology and EEG technicians. Health-care providers completed extensive epilepsy care surveys. The five countries all have integrated government health systems with clinics and hospitals that provide free or heavily subsidized care and AEDs for patients with epilepsy. Only Trinidad and Tobago and Barbados, however, have neurology specialists. The three smaller countries lack government imaging and EEG facilities. Trinidad had up to one-year waits for public MRI/EEG. Government formularies in Grenada, Saint Vincent and the Grenadines, and Saint Lucia are limited to first-generation AEDs. One or more second-line agents are formulary in Trinidad and Barbados. Nonformulary drugs may be obtained for individual patients in Barbados. Grenada, Saint Lucia, and Saint Vincent and the Grenadines participate in an Organization of Eastern Caribbean States formulary purchasing system, which added levetiracetam following the survey. Newer generic AED formulations with the lowest risks for pregnancy malformation were not in use. In conclusion, patients with epilepsy in the southern Caribbean have excellent access to government clinics and hospitals, but AED choices

  19. Plutonium in Southern Hemisphere ocean Waters

    DEFF Research Database (Denmark)

    Hirose, K.; Aoyama, M.; Gastaud, J.

    2013-01-01

    Plutonium in seawater collected by the BEAGLE2003 cruise was determined using ICP- SF-MS and alpha spectrometry after Fe co-precipitation and radiochemical purification. Levels and distributions of dissolved plutonium activity concentrations in Southern Hemisphere ocean waters are summarized here......, including historical data. Pu-239 concentrations in surface water----of the central South Pacific (32.5 °S) in 2003 were around 1 mBq/m3. The 239Pu concentrations in the Indian Ocean surface waters (20°S) were similar to that in the South Pacific, whereas the 239Pu concentrations in the South Atlantic...... surface waters (30°S) were markedly lower than those in the South Pacific and Indian Oceans. The 239Pu vertical profile pattern was similar to that in the North Pacific subtropical gyre, although 239Pu concentrations in the deep South Pacific were significantly lower than those in the North Pacific. One...

  20. [Toxicity and apple production in southern Brazil].

    Science.gov (United States)

    Klanovicz, Jó

    2010-03-01

    The article explores the links between the controversial apprehension of contaminated apples in southern Brazil in 1989 and the reactions of the apple industry to press reports on the use of pesticides in Brazilian orchards. The issue is framed within a broader analysis of the notions of toxicity and 'danger' surrounding the consumption of healthier food and the idea of 'food security,' notions that have begun taking hold in public and private life. It is argued that apple growers' responses to the problem can be better understood through a historical reading of the interactions between the biology of the apple tree, the agroecology of this monoculture, and the structures, actors, and discourses of the human and non-human groups in Brazil's apple-producing region.

  1. Ciguatera fish poisoning. A southern California epidemic.

    Science.gov (United States)

    Barton, E D; Tanner, P; Turchen, S G; Tunget, C L; Manoguerra, A; Clark, R F

    1995-01-01

    Ciguatera fish poisoning results from the bioconcentration of a variety of toxins produced by marine dinoflagellates. Signs and symptoms vary widely, but it usually presents as gastrointestinal and neurologic complaints beginning shortly after the ingestion of fish containing the toxins. Symptoms may persist for months and sometimes even years. Although cases have been reported throughout the United States, epidemics are most common along tropical and subtropical coasts and usually involve the ingestion of large carnivorous fish. We review the literature and report the first epidemic of 25 cases of ciguatera fish poisoning presenting to area hospitals in Southern California that were successfully tracked by the Department of Health Services and isolated to fish caught off the coast of Baja California, Mexico. Images Figure 1. PMID:7667980

  2. Prevalence of Asthma in Southern Punjab, Pakistan.

    Directory of Open Access Journals (Sweden)

    Maryum Khan

    2016-01-01

    Full Text Available AbstractAsthma is exaggerated response of immune system which is a leading cause of death in the third world. Main causes of asthma are allergy, smoking, drugs like NSAID (Aspirin and family history. Objective of study was to check the prevalence of asthma in different age groups and its impact on socioeconomical behaviors of the peoples of southern Punjab, by developing a questionnaire. Incidence of asthmatic attack in the age group of 20 to 60 years was more than in age group of 20 years, furthermore the incidence was found to be more common in females as compare to males. The smokers were at more risk to develop the disease as compared to the nonsmokers.

  3. Neutral hydrogen observations of southern galaxies

    International Nuclear Information System (INIS)

    Davies, R.D.; Staveley-Smith, L.; Murray, J.D.

    1989-01-01

    21-cm neutral hydrogen observations, made using the 64-m Parkes radio telescope, are presented for 48 galaxies, 39 of which are clear detections. The H I properties and homogeneous optical data, including new photometry, are tabulated for the galaxies. A high signal-to-noise ratio subset of these observations is used to demonstrate that the overall flux-density scale is within a few per cent of northern (Jodrell Bank and Green Bank) scales. The remainder are used elsewhere in a new determination of the Local Group peculiar velocity. H I-optical diameter correlations are shown to be a useful discriminant between competing diameter systems and indicate that the Lauberts ESO(B) catalogue is the most useful currently available source of southern galaxy diameters. (author)

  4. A phytogeographic survey of Southern Benin

    Directory of Open Access Journals (Sweden)

    G. Paradis

    1983-11-01

    Full Text Available Southern Benin has a dry subequatoriai climate with a rainfall gradient from 850 mm in the west to 1 500 mm in the east, the geomorphology is varied and the vegetation has been subjected to strong human influence. There are numerous plant formations, namely: 1, forest islands which are probably relics of the primitive vegetation and include (a dense semi-deciduous forests of several types, (b swamp forests of two types, (c periodically flooded forest of two types, (d Lophira lanceolata  (Hutchinson & Dalziel, 1954-72 woodlands and (e mangrove swamps; 2, formations which are probably derived and include (a thickets of several types, (b tree savannas and shrub savannas, (c grassy savannas and prairies varying according to soil characteristics and (d halophytic grasslands; and 3, floating vegetation on fresh-water lakes.

  5. SOUTH POL: Revealing the Polarized Southern Sky

    Science.gov (United States)

    Magalhaes, Antonio Mario Mario; Ramírez, Edgar; Ribeiro, Nadili; Seriacopi, Daiane; Rubinho, Marcelo; Ferrari, Tiberio; Rodrigues, Claudia; Schoenell, William; Herpich, Fabio; Pereyra, Antonio

    2018-01-01

    SOUTH POL will be a survey of the Southern sky in optical polarized light. It will use a newly built polarimeter for T80-S, an 84 cm robotic telescope installed at Cerro Tololo (CTIO), Chile. It will initially cover the sky South of declination -15 deg with a polarimetric accuracy Solar System.The polarimeter has just been commissioned in mid-November, 2017. The data reduction pipeline has already been built. We will describe the instrument and the data reduction, as well as a few of the science cases. The survey is expected to begin midway through the 1st semester of 2018. Both catalog data and raw images will be made available.

  6. Southern marl prairies conceptual ecological model

    Science.gov (United States)

    Davis, S.M.; Loftus, W.F.; Gaiser, E.E.; Huffman, A.E.

    2005-01-01

    About 190,000 ha of higher-elevation marl prairies flank either side of Shark River Slough in the southern Everglades. Water levels typically drop below the ground surface each year in this landscape. Consequently, peat soil accretion is inhibited, and substrates consist either of calcitic marl produced by algal periphyton mats or exposed limestone bedrock. The southern marl prairies support complex mosaics of wet prairie, sawgrass sawgrass (Cladium jamaicense), tree islands, and tropical hammock communities and a high diversity of plant species. However, relatively short hydroperiods and annual dry downs provide stressful conditions for aquatic fauna, affecting survival in the dry season when surface water is absent. Here, we present a conceptual ecological model developed for this landscape through scientific concensus, use of empirical data, and modeling. The two major societal drivers affecting the southern marl prairies are water management practices and agricultural and urban development. These drivers lead to five groups of ecosystem stressors: loss of spatial extent and connectivity, shortened hydroperiod and increased drought severity, extended hydroperiod and drying pattern reversals, introduction and spread of non-native trees, and introduction and spread of non-native fishes. Major ecological attributes include periphyton mats, plant species diversity and community mosaic, Cape Sable seaside sparrow (Ammodramus maritimus mirabilis), marsh fishes and associated aquatic fauna prey base, American alligator (Alligator mississippiensis), and wading bird early dry season foraging. Water management and development are hypothesized to have a negative effect on the ecological attributes of the southern marl prairies in the following ways. Periphyton mats have decreased in cover in areas where hydroperiod has been significantly reduced and changed in community composition due to inverse responses to increased nutrient availability. Plant species diversity and

  7. Neonatal screening for hypothyroidism in Southern Thailand

    International Nuclear Information System (INIS)

    Sukthomya, V.

    1985-08-01

    Thyroid stimulating hormone (TSH) determination by a radioimmunoassay on dried blood spots was selected as the screening method for congenital hypothyroidism in Southern Thailand. Blood from the infant's heel was obtained in 7814 newborns on the fifth day of life. Infants with TSH values greater than 25 mU/L were recalled for a more careful clinical examination and to have their T4 (thyroxine) and TSH re-estimated. Fifty-four infants with TSH values between 25 and 50 mU/L were found to be normal at re-evaluation. These are still being followed longitudinally for full confirmation. Two with values above 50 mU/L, although clinically euthyroid, were found to be hypothyroid by T3, T4, thyroid scintigraphy and bone age on roentgenography. Replacement therapy has been started. We have shown that a screening program for neonatal hypothyroidism can be done, and is probably worthwhile and perhaps even cost-effective

  8. Southern California Edison instrument setpoint program

    International Nuclear Information System (INIS)

    Bockhorst, R.M.; Quinn, E.L.

    1991-01-01

    In November of 1989, the US Nuclear Regulatory Commission (NRC) conducted an electrical safety system functional inspection (ESSFI) at the San Onofre nuclear generating station (SONGS), which was followed by an NRC audit on instrument setpoint methodology in January 1991. Units 2 and 3 at SONGS are 1100-MW(electric) Combustion Engineering (C-E) pressurized water reactors (PWRs) operated by Southern California Edison (SCE). The purpose of this paper is to summarize the results of the NRC audit and SCE's follow-up activities. The NRC team inspection reinforced the need to address several areas relative to the SCE setpoint program. The calculations withstood the intensive examination of four NRC inspectors for 2 weeks and only a few minor editorial-type problems were noted. Not one of the calculated plant protections system setpoints will change as a result of the audit. There were no questions raised relative to setpoint methodology

  9. Some isotope hydrological studies in Southern Africa

    International Nuclear Information System (INIS)

    Verhagen, B.Th.

    2001-01-01

    Four case studies involving the use of the environmental isotopes 14 C and 3 H, in the and to semi-arid Kalahari region of Southern Africa are described and general conclusions regarding the qualitative aspects of recharge and discharge characteristics of the systems are based on these measurements. In each of the studies, diffuse, local recharge was found to be the dominant recharge mechanism. Recharge via river beds was found to be limited at the regional scale. The balancing discharge mechanism for groundwater was found to be via evapotranspiration. Groundwater salinity and mineralisation as well as the regional hydrogeology are controlled by geological structure rather than lithologies or residence times and the absence of hypersaline groundwaters indicates that the aquifers are periodically flushed during pluvial periods, thus pointing to long-term hydroclimatic controls over the observed present-day hydrology. (author)

  10. Brazil to Join the European Southern Observatory

    Science.gov (United States)

    2010-12-01

    The Federative Republic of Brazil has yesterday signed the formal accession agreement paving the way for it to become a Member State of the European Southern Observatory (ESO). Following government ratification Brazil will become the fifteenth Member State and the first from outside Europe. On 29 December 2010, at a ceremony in Brasilia, the Brazilian Minister of Science and Technology, Sergio Machado Rezende and the ESO Director General, Tim de Zeeuw signed the formal accession agreement aiming to make Brazil a Member State of the European Southern Observatory. Brazil will become the fifteen Member State and the first from outside Europe. Since the agreement means accession to an international convention, the agreement must now be submitted to the Brazilian Parliament for ratification [1]. The signing of the agreement followed the unanimous approval by the ESO Council during an extraordinary meeting on 21 December 2010. "Joining ESO will give new impetus to the development of science, technology and innovation in Brazil as part of the considerable efforts our government is making to keep the country advancing in these strategic areas," says Rezende. The European Southern Observatory has a long history of successful involvement with South America, ever since Chile was selected as the best site for its observatories in 1963. Until now, however, no non-European country has joined ESO as a Member State. "The membership of Brazil will give the vibrant Brazilian astronomical community full access to the most productive observatory in the world and open up opportunities for Brazilian high-tech industry to contribute to the European Extremely Large Telescope project. It will also bring new resources and skills to the organisation at the right time for them to make a major contribution to this exciting project," adds ESO Director General, Tim de Zeeuw. The European Extremely Large Telescope (E-ELT) telescope design phase was recently completed and a major review was

  11. Early ceramic sites in southern Lau, Fiji

    International Nuclear Information System (INIS)

    Clark, G.

    2009-01-01

    The Lau Group is a dispersed chain of islands in east Fiji trending north-south on the inactive Lau Ridge remnant volcanic arc, which is part of the convergent margin system comprising the Lau Basin and Tonga Ridge to the east. In the south of the Lau Group are several remote islands that are closer to Tangatapu than they are to the largest islands of Viti Levu and Vanua Levu in the Fiji Group. Two of these islands, Vatoa and Doi islands in the Ono-i-Lau Group, were visited briefly in 2006 after field work on Kabara when the government supply boat 'Sandy' made scheduled stops to unload and collect cargo. Lapita pottery and a fortification were recorded on Doi Island in the Ono Group, and on Vatoa Island a site with plain ware ceramics dating to c. 2000 cal. BP was located. The position of the two remote islands lying between the main islands of Fiji and Tongan archipelago suggests they were particularly subject to influence from population contacts and movements from islands to their east and west in prehistory. In the eighteenth and nineteenth century central and southern Lau were the centre of an important canoe building industry based on the hardwood Instia bijuga (vesi) used to construct large sailing canoes which were the prerogative of Tongan and Fijian chiefs. Archaeological and linguistic studies of the Lau Group suggest a complicated history of population movements earlier during Lapita (2900-2500 BP) and post-Lapita (∼2500-1000 BP) times, which can be examined from the study of ceramic sites in southern Lau. (author). 27 refs., 3 figs

  12. Biogeochemistry of southern Australian continental slope sediments

    International Nuclear Information System (INIS)

    Veeh, H.H.; Crispe, A.J.; Heggie, D.T.

    1999-01-01

    Sediment cores from the middle to lower slope of the southern continental margin of Australia between the Great Australian Bight and western Tasmania are compared in terms of marine and terrigenous input signals during the Holocene. The mass accumulation rates of carbonate, organic carbon, biogenic Ba. and Al are corrected for lateral sediment input (focusing), using the inventory of excess 230 Th in the sediment normalised to its known production rate in the water column above each site. The biogenic signal is generally higher in the eastern part of the southern margin probably due to enhanced productivity associated with seasonal upwelling off southeastern South Australia and the proximity of the Subtropical Front, which passes just south of Tasmania. The input of Al, representing the terrigenous signal, is also higher in this region reflecting the close proximity of river runoff from the mountainous catchment of southeastern Australia. The distribution pattern of Mn and authigenic U, together with pore-water profiles of Mn ++ , indicate diagenetic reactions driven by the oxidation of buried organic carbon in an oxic to suboxic environment. Whereas Mn is reduced at depth and diffuses upwards to become immobilised in a Mn-rich surface layer. U is derived from seawater and diffuses downward into the sediment, driven by reduction and precipitation at a depth below the reduction zone of Mn. The estimated removal rate of U from seawater by this process is within the range of U removal measured in hemipelagic sediments from other areas, and supports the proposition that hemipelagic sediments are a major sink of U in the global ocean. Unlike Mn, the depth profile of sedimentary Fe appears to be little affected by diagenesis, suggesting that little of the total Fe inventory in the sediment is remobilised and redistributed as soluble Fe. Copyright (1999) Blackwell Science Pty Ltd

  13. SRAO: the first southern robotic AO system

    Science.gov (United States)

    Law, Nicholas M.; Ziegler, Carl; Tokovinin, Andrei

    2016-08-01

    We present plans for SRAO, the first Southern Robotic AO system. SRAO will use AO-assisted speckle imaging and Robo-AO-heritage high efficiency observing to confirm and characterize thousands of planet candidates produced by major new transit surveys like TESS, and is the first AO system to be capable of building a comprehensive several-thousand-target multiplicity survey at sub-AU scales across the main sequence. We will also describe results from Robo-AO, the first robotic LGS-AO system. Robo-AO has observed tens of thousands of Northern targets, often using a similar speckle or Lucky-Imaging assisted mode. SRAO will be a moderate-order natural-guide-star adaptive optics system which uses an innovative photoncounting wavefront sensor and EMCCD speckle-imaging camera to guide on faint stars with the 4.1m SOAR telescope. The system will produce diffraction-limited imaging in the NIR on targets as faint as mν = 16. In AO-assisted speckle imaging mode the system will attain the 30-mas visible diffraction limit on targets at least as faint as mν = 17. The system will be the first Southern hemisphere robotic adaptive optics system, with overheads an order of magnitude smaller than comparable systems. Using Robo-AO's proven robotic AO software, SRAO will be capable of observing overheads on sub-minute scales, allowing the observation of at least 200 targets per night. SRAO will attain three times the angular resolution of the Palomar Robo-AO system in the visible.

  14. Is wetland mitigation successful in Southern California?

    Science.gov (United States)

    Cummings, D. L.; Rademacher, L. K.

    2004-12-01

    Wetlands perform many vital functions within their landscape position; they provide unique habitats for a variety of flora and fauna and they act as treatment systems for upstream natural and anthropogenic waste. California has lost an estimated 91% of its wetlands. Despite the 1989 "No Net Loss" policy and mitigation requirements by the regulatory agencies, the implemented mitigation may not be offsetting wetlands losses. The "No Net Loss" policy is likely failing for numerous reasons related to processes in the wetlands themselves and the policies governing their recovery. Of particular interest is whether these mitigation sites are performing essential wetlands functions. Specific questions include: 1) Are hydric soil conditions forming in mitigation sites; and, 2) are the water quality-related chemical transformations that occur in natural wetlands observed in mitigation sites. This study focuses on success (or lack of success) in wetlands mitigation sites in Southern California. Soil and water quality investigations were conducted in wetland mitigation sites deemed to be successful by vegetation standards. Observations of the Standard National Resource Conservation Service field indicators of reducing conditions were made to determine whether hydric soil conditions have developed in the five or more years since the implementation of mitigation plans. In addition, water quality measurements were performed at the inlet and outlet of these mitigation sites to determine whether these sites perform similar water quality transformations to natural wetlands within the same ecosystem. Water quality measurements included nutrient, trace metal, and carbon species measurements. A wetland location with minimal anthropogenic changes and similar hydrologic and vegetative features was used as a control site. All sites selected for study are within a similar ecosystem, in the interior San Diego and western Riverside Counties, in Southern California.

  15. Characteristics of southern California atmospheric rivers

    Science.gov (United States)

    Harris, Sarah M.; Carvalho, Leila M. V.

    2018-05-01

    Atmospheric rivers (ARs) are channels of high water vapor flux that transport moisture from low to higher latitudes on synoptic timescales. In areas of topographical variability, ARs may lead to high-intensity precipitation due to orographic forcing. ARs landfalling along North America's west coast are linked to extreme events including those leading to flooding and landslides. In southern California (SCA), proper AR forecasting is important for regional water resources as well as hazard mitigation and as the area's annual precipitation totals occur from relatively few storms per season, any changes to storm frequency and/or intensity may have dramatic consequences. Yet, as most regional AR studies focus on the Pacific Northwest, there is little information about SCA ARs. We develop an algorithm to identify ARs landfalling on North America's west coast between 1979 and 2013 within total precipitable water reanalysis fields. ARs are then categorized according to landfall region. To determine and differentiate the characteristics and spatial distributions of ARs affecting these areas, we examine lag composites of various atmospheric variables for each landfall region. SCA ARs differ from ARs landfalling farther north in the days prior to landfall with the position and amplitude of a trough offshore from the Asian continent and ridge over Alaska, as well as the displacement and eastward extension of the jet core that potentially guides AR moisture southwards. The relationships between AR landfalls and the El Niño/Southern Oscillation (ENSO), the Madden-Julian Oscillation (MJO), and the Pacific/North American Teleconnection Pattern (PNA) are also investigated.

  16. Extratropical Cyclone in the Southern Ocean

    Science.gov (United States)

    2002-01-01

    These images from the Multi-angle Imaging SpectroRadiometer (MISR) portray an occluded extratropical cyclone situated in the Southern Ocean, about 650 kilometers south of the Eyre Peninsula, South Australia. The left-hand image, a true-color view from MISR's nadir (vertical-viewing) camera, shows clouds just south of the Yorke Peninsula and the Murray-Darling river basin in Australia. Retrieved cloud-tracked wind velocities are indicated by the superimposed arrows. The image on the right displays cloud-top heights. Areas where cloud heights could not be retrieved are shown in black. Both the wind vectors and the cloud heights were derived using data from multiple MISR cameras within automated computer processing algorithms. The stereoscopic algorithms used to generate these results are still being refined, and future versions of these products may show modest changes. Extratropical cyclones are the dominant weather system at midlatitudes, and the term is used generically for regional low-pressure systems in the mid- to high-latitudes. In the southern hemisphere, cyclonic rotation is clockwise. These storms obtain their energy from temperature differences between air masses on either side of warm and cold fronts, and their characteristic pattern is of warm and cold fronts radiating out from a migrating low pressure center which forms, deepens, and dissipates as the fronts fold and collapse on each other. The center of this cyclone has started to decay, with the band of cloud to the south most likely representing the main front that was originally connected with the cyclonic circulation. These views were acquired on October 11, 2001, and the large view represents an area of about 380 kilometers x 1900 kilometers. Image courtesy NASA/GSFC/LaRC/JPL, MISR Team.

  17. A methodology for assessing annual risk of southern pine beetle outbreaks across the southern region using pheromone traps

    Science.gov (United States)

    Ronald F Billings; William W. Upton

    2010-01-01

    An operational system to forecast infestation trends (increasing, static, declining) and relative population levels (high, moderate, low) of the southern pine beetle (SPB), Dendroctonus frontalis, has been implemented in the Southern and Eastern United States. Numbers of dispersing SPB and those of a major predator (the clerid beetle, ...

  18. Blue-stain Fungi Associated with Roots of Southern Pine Trees Attacked by the Southern Pine Beetle, Dendroctonus frontalis

    Science.gov (United States)

    William J. Otrosina; Nolan J. Hess; Stanley J. Zarnoch; Thelma J. Perry; John P. Jones

    1997-01-01

    Forty paired plots were established from eastern Texas to Alabama to study root-infecting, blue-stain fungi in southern pine stands undergoing southern pine beetle (SPB) attack. Woody roots were sampled in plots undergoing recent or current attack by the SPB. Comparisons were made between occurrence of Lcptogrqhiumspp. and related fungi and data on various...

  19. Northerners versus southerners: Italian anthropology and psychology faced with the "southern question".

    Science.gov (United States)

    Cimino, Guido; Foschi, Renato

    2014-11-01

    Following the Unification of Italy (1861), when confronted with the underdevelopment problems of the south that had given rise to the so-called "southern question," some Italian anthropologists and psychologists began to study the populations of the south from the psycho-anthropological point of view. These scientists, at times subject to preconceived ideas toward the southerners, conveyed observations and descriptions of the southern character traits that, in general, were considered different, in a negative sense, with respect to those of the northern peoples. To explain such diversity in the "psychological" characteristics between the north and south of the country (presumed cause also of the south's backwardness), various hypotheses were advanced related to the kind of heredity theory adopted, which could be of, more or less, an "innatist" or "transformist" or "environmentalist" kind. The distinction proposed in this article between at least 2 different "hereditarian" theories formulated by the Italian scientists, and the confrontation of these theories with the hypotheses expressed by the "southernist" sociologists, contrary to the idea of "racial varieties" present in the Italian population, allows one to understand in what way and in what sense, at the threshold of the 20th century, there arose the ideology of "Nordicism" and the roots of racism were planted.

  20. Monitoring natural vegetation in Southern Greenland using NOAA AVHRR and field measurements

    DEFF Research Database (Denmark)

    Hansen, Birger Ulf

    1991-01-01

    vegetation, sheep farming, biomass production, Remote Sensing, NOAA AVHRR, Southern Greenland, NDVI......vegetation, sheep farming, biomass production, Remote Sensing, NOAA AVHRR, Southern Greenland, NDVI...

  1. Ocean Acidification Effects on the Early Life-Stages of Commercially Important Flatfish of the Northeast USA

    Science.gov (United States)

    Chambers, R. C.; Habeck, E. A.; Candelmo, A. C.; Poach, M.; Wieczorek, D.; Phelan, B.; Caldarone, E.; Cooper, K. R.

    2012-12-01

    The limited available evidence about effects on finfish of high CO2 levels and acidification of our oceans suggests that effects will differ across fish species, be subtle, and interact with other stressors. A carefully planned, experimental framework was developed to cast an extensive yet strategic inferential net. Three key elements of our approach are the use of 1) multiple marine finfish species of relevance to the northeastern USA that differ in their ecologies including spawning season and habitat of early life-stages; 2) a wide yet realistic range of environmental conditions (i.e., concurrent manipulation of CO2 levels and water temperatures), and 3) a diverse set of response variables related to fish sensitivity to elevated CO2 levels, water temperatures, and their interactions. The response variable set reflects fish condition, fitness, and likelihood of recruitment, and includes measures of viability, physiology, histopathology, growth, development, and behavior expressed during fish early life-stages (i.e., gametes, embryos, and larvae). Early life-stages were chosen due to the anticipation of their vulnerability to acid-base challenges in their environment. To date, factorial experiments have been implemented on summer flounder (Paralichthys dentatus) and winter flounder (Pseudopleuronectes americanus). Initial results reveal survival of summer flounder embryos is compromised by pH 790 ppm). These results were similar across offspring groups (i.e., embryos from different parents). Winter flounder are larger at hatching when exposed to high CO2 levels in the coolest environment implemented in our experiments (range 4 to 10 ○C). Further responses of advanced larvae of both flounder species are currently being assessed for evidence of other whole body, component organ, and biochemical impairment. This study will aid researchers and resource managers in identifying species types, life-stages, and biotic responses that are most sensitive to the expected

  2. Clean Air Slots Amid Dense Atmospheric Pollution in Southern Africa

    Science.gov (United States)

    Hobbs, Peter V.

    2003-01-01

    During the flights of the University of Washington's Convair-580 in the Southern African Regional Science Initiative (SAFARI 2000) in southern Africa, a phenomenon was observed that has not been reported previously. This was the occurrence of thin layers of remarkably clean air, sandwiched between heavily polluted air, which persisted for many hours during the day. Photographs are shown of these clean air slots (CAS), and particle concentrations and light scattering coefficients in and around such slot are presented. An explanation is proposed for the propensity of CAS to form in southern Africa during the dry season.

  3. Severe wind phenomena in Southern Africa and the related damage

    CSIR Research Space (South Africa)

    Goliger, Adam M

    2007-10-01

    Full Text Available ) and the major factor promoting the spread of bushfires. 3. Origin and character of severe wind storms A schematic map of the occurrence of strong wind events over southern Africa, based on Griffith (1972), Lundholm (1979), Goliger and Milford (1998... the southern tip of the sub- continent and originate in extra-tropical cyclones. 3. Shallow south-easters, which develop as a result of localised coastal lows. Their influence extends along the western and southern coast of South Africa, from Luderitz...

  4. Stars and Seasons in Southern Africa

    Science.gov (United States)

    Snedegar, K. V.

    Although the indigenous people of Southern Africa traditionally viewed the sky as a place quite apart from the Earth, they believed celestial phenomena to be natural signs united with those of the Earth in a harmonious synchronicity. There is no substantial evidence that the precolonial Africans imagined a casual relationship between celestial bodies and the seasonal patterns of life on Earth. They did, however, recognize a coincidental relationship. The traditional African cosmos, then, worked as a noetic principle unifying the observed motions of celestial bodies, the sequence of seasons, and the behavior of plants and animals. Such a cosmos, with local peculiarities, was widely understood in Southern Africa before the end of the last century. By the early 20th century European colonial paradigms had largely obliterated this African worldview. This paper will offer a partial reconstruction. Pre-colonial South African people viewed time as a sequence of discrete natural events; through annual repetition these events served as a guide for proper human action. The South Africans analyzed the passage of time in terms of the motions of celestial bodies, the maturation of beneficial plants, and the mating patterns of animals. The rightful course of human life was seen to fit within the seasonal context of these natural phenomena. The visibility of conspicuous stars and asterisms marked significant times of year. For instance, the Lovedu people greeted the dawn rising of Canopus with joy: "The boy has come out." The star was a signal for rainmaking and boys' initiation ceremonies to proceed. The Venda constellation Thutlwa, the giraffes, comprises α and β Crucis and α and β Centauri. In October Thutlwa skims the trees of the evening horizon. The Venda Thutlwa literally means 'rising above the trees,' an allusion to the majestic vegetarian creatures and the stars advising the people to be done with their spring planting. This paper will describe stellar associations

  5. Mechanization in firewood harvesting in southern Italy

    Directory of Open Access Journals (Sweden)

    2005-01-01

    Full Text Available The aim of this research was to survey current mechanization level of coppice harvesting in Southern Italy. The cooperation of the General Direction of the National Forest Service (NFS has been a basic tool of survey. A questionnaire compiled on purpose was sent to each Forest Station (hereinafter referred to as CS in the following regions: Basilicata, Campania and Calabria. A high percentage (80% of the CSs did fulfill the questionnaire. The answers highlight that: i the main assortment currently produced is firewood; ii the level of harvesting mechanization is rather low, equipment being quite obsolete: indeed, the most widely used machineries are farm tractors partly adapted to forest harvesting and equipped with cages or back winch; iii the use of animals for hauling (mules and oxen, the latter in Calabria is still quite frequent, while forest tractors, polyethylene chutes and cable cranes are almost absent; iv the use of individual protection (DPI and machinery protection devices (DPM is on average quite low.

  6. Alboran Basin, southern Spain - Part I: Geomorphology

    Energy Technology Data Exchange (ETDEWEB)

    Munoz, A. [Secretaria General de Pesca Maritima, Corazon de Maria, 8, 28002 Madrid (Spain); Ballesteros, M.; Rivera, J.; Acosta, J. [Instituto Espanol de Oceanografia, Corazon de Maria, 8, 28002 Madrid (Spain); Montoya, I. [Universidad Juan Carlos I, Campus de Mostoles, Madrid (Spain); Uchupi, E. [Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States)

    2008-01-15

    Bathymetric, 3D relief and shaded relief maps created from multibeam echo-sounding data image the morphology of the Alboran Basin, a structural low along the east-west-trending Eurasian-African plates boundary. Topographic features in the basin are the consequence of volcanism associated with Miocene rifting, rift and post-rift sedimentation, and recent faulting resulting from the convergence of the African-Eurasian plates. Pleistiocene glacially induced regressions/transgressions when the sea level dropped to about 150 m below its present level gas seeps and bottom currents. Recent faulting and the Pleistocene transgressions/regressions led to mass-wasting, formation of turbidity currents and canyon erosion on the basin's slopes. Recent fault traces at the base of the northern basin slope have also served as passageways for thermogenic methane, the oxidation of which by bacteria led to the formation of carbonate mounds along the fault intercepts on the sea floor. Expulsion of thermogenic or biogenic gas has led to the formation of pockmarks; erosion by bottom currents has resulted in the formation of moats around seamounts and erosion of the seafloor of the Alboran Ridge and kept the southern edge of the 36 10'N high sediment free. (author)

  7. Wastewater effluent dispersal in Southern California Bays

    KAUST Repository

    Uchiyama, Yusuke; Idica, Eileen Y.; McWilliams, James C.; Stolzenbach, Keith D.

    2014-01-01

    The dispersal and dilution of urban wastewater effluents from offshore, subsurface outfalls is simulated with a comprehensive circulation model with downscaling in nested grid configurations for San Pedro and Santa Monica Bays in Southern California during Fall of 2006. The circulation is comprised of mean persistent currents, mesoscale and submesoscale eddies, and tides. Effluent volume inflow rates at Huntington Beach and Hyperion are specified, and both their present outfall locations and alternative nearshore diversion sites are assessed. The effluent tracer concentration fields are highly intermittent mainly due to eddy currents, and their probability distribution functions have long tails of high concentration. The dilution rate is controlled by submesoscale stirring and straining in tracer filaments. The dominant dispersal pattern is alongshore in both directions, approximately along isobaths, over distances of more than 10. km before dilution takes over. The current outfall locations mostly keep the effluent below the surface and away from the shore, as intended, but the nearshore diversions do not. © 2014 Elsevier Ltd.

  8. Eimeria infections in goats in Southern Portugal

    Directory of Open Access Journals (Sweden)

    Liliana Machado Ribeiro da Silva

    Full Text Available Coccidiosis caused by Eimeria species is a major form of intestinal infection affecting intensively and semi-intensively reared goats. The province of Alentejo is the main goat-producing area in Portugal. Therefore, all 15 Serpentina goat farms in Alentejo were analyzed regarding the occurrence and diversity of Eimeria species. Fecal samples obtained from 144 animals (52.1% dairy goats, 47.9% pre-pubertal goats were examined using the modified McMaster technique to determine the number of oocysts per gram of feces. Eimeria spp. oocysts were present in 98.61% of the fecal samples and, overall, nine different Eimeria species were identified. The most prevalent species were E. ninakohlyakimovae (88% and E. arloingi (85%, followed by E. alijevi (63% and E. caprovina(63%. The average number of oocysts shed was significantly lower in dairy goats than in pre-adult animals. Astonishingly, no clinical signs of coccidiosis were observed in any of the animals examined, even though they were shedding high numbers of oocysts and were infected with highly pathogenic species. Thus, implementation of routine diagnostic investigation of the occurrence and diversity of caprine Eimeria species may be a useful tool for determination and better understanding of their potential economic impact on goat herds in southern Portugal.

  9. Crotalid envenomation: the southern Arizona experience.

    Science.gov (United States)

    Tokish, J T; Benjamin, J; Walter, F

    2001-01-01

    To review a regional experience with the treatment of snakebites. Five major southern Arizona hospitals, including two Level I trauma centers. A review of all snakebite admissions over a five-year period was performed. During the period reviewed, 164 patients were admitted for snakebites. Rattlesnakes were responsible for 98 percent of identified envenomations. Thirty-six percent of the patients were transported by air to the admitting facility. Eighty percent of patients were admitted to the intensive care unit for an average of 1.6 days. Total hospital stays averaged 2.8 days. Ninety percent of patients received antivenin, usually only on the day of admission. Of those receiving antivenin, 20 percent had an anaphylactoid reaction, and 1 percent required readmission for serum sickness. Laboratory evaluation indicated abnormalities in platelet count, coagulation parameters, and fibrinogen levels, but these rarely required treatment. Thirteen percent of patients underwent surgical intervention, including a 4 percent fasciotomy rate, and a single amputation. The use of field treatment, including "cut and suck," tourniquets, and cryotherapy, increased the likelihood of surgery. The authors concluded that the intensive care unit and helicopter transport system were overused. They recommend that established objective envenomation severity scores be used to dictate patient treatment, specifically the use of antivenin.

  10. The Southern HII Region Discovery Survey

    Science.gov (United States)

    Wenger, Trey; Miller Dickey, John; Jordan, Christopher; Bania, Thomas M.; Balser, Dana S.; Dawson, Joanne; Anderson, Loren D.; Armentrout, William P.; McClure-Griffiths, Naomi

    2016-01-01

    HII regions are zones of ionized gas surrounding recently formed high-mass (OB-type) stars. They are among the brightest objects in the sky at radio wavelengths. HII regions provide a useful tool in constraining the Galactic morphological structure, chemical structure, and star formation rate. We describe the Southern HII Region Discovery Survey (SHRDS), an Australia Telescope Compact Array (ATCA) survey that discovered ~80 new HII regions (so far) in the Galactic longitude range 230 degrees to 360 degrees. This project is an extension of the Green Bank Telescope HII Region Discovery Survey (GBT HRDS), Arecibo HRDS, and GBT Widefield Infrared Survey Explorer (WISE) HRDS, which together discovered ~800 new HII regions in the Galactic longitude range -20 degrees to 270 degrees. Similar to those surveys, candidate HII regions were chosen from 20 micron emission (from WISE) coincident with 10 micron (WISE) and 20 cm (SGPS) emission. By using the ATCA to detect radio continuum and radio recombination line emission from a subset of these candidates, we have added to the population of known Galactic HII regions.

  11. The buffalo in Southern South America

    Directory of Open Access Journals (Sweden)

    M. Zava

    2010-02-01

    Full Text Available The number of buffaloes in the American continent is growing at the explosive rate of 12.7 % a year, well above all other continents. Within this context the different situations of the southern part of South America are described, those countries that two hundred years ago were part of the River Plate Viceroyship and the Chile General Command, both of them part of the Spanish empire. The first steps of buffaloes in Paraguay, Bolivia and Uruguay. The expansion of buffaloes in Argentina and their recent start up in Chile. The production systems in the region are described, principally Argentina and Paraguay. The major changes and expansion of agriculture in Argentina are displacing cattle production from the North towards the Tropics, where buffalo has proven to be very superior in productivity compared to cattle production thanks to its very good adaptation to the local conditions. In Paraguay, a totally subtropical country, something very similar is happening. Paraguay has consolidated its sales of buffalo hides and beef. Argentina is well on its way to having a very efficient buffalo beef marketing competing with cattle of the highest quality. Buffalo milk production is still not mature in these countries, although there are several projects underway in Uruguay, Bolivia and Argentina.

  12. Southern Regional Center for Lightweight Innovative Design

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Paul T. [Mississippi State Univ., Mississippi State, MS (United States)

    2012-12-01

    The Southern Regional Center for Lightweight Innovative Design (SRCLID) has developed an experimentally validated cradle-to-grave modeling and simulation effort to optimize automotive components in order to decrease weight and cost, yet increase performance and safety in crash scenarios. In summary, the three major objectives of this project are accomplished: To develop experimentally validated cradle-to-grave modeling and simulation tools to optimize automotive and truck components for lightweighting materials (aluminum, steel, and Mg alloys and polymer-based composites) with consideration of uncertainty to decrease weight and cost, yet increase the performance and safety in impact scenarios; To develop multiscale computational models that quantify microstructure-property relations by evaluating various length scales, from the atomic through component levels, for each step of the manufacturing process for vehicles; and To develop an integrated K-12 educational program to educate students on lightweighting designs and impact scenarios. In this final report, we divided the content into two parts: the first part contains the development of building blocks for the project, including materials and process models, process-structure-property (PSP) relationship, and experimental validation capabilities; the second part presents the demonstration task for Mg front-end work associated with USAMP projects.

  13. Wastewater effluent dispersal in Southern California Bays

    KAUST Repository

    Uchiyama, Yusuke

    2014-03-01

    The dispersal and dilution of urban wastewater effluents from offshore, subsurface outfalls is simulated with a comprehensive circulation model with downscaling in nested grid configurations for San Pedro and Santa Monica Bays in Southern California during Fall of 2006. The circulation is comprised of mean persistent currents, mesoscale and submesoscale eddies, and tides. Effluent volume inflow rates at Huntington Beach and Hyperion are specified, and both their present outfall locations and alternative nearshore diversion sites are assessed. The effluent tracer concentration fields are highly intermittent mainly due to eddy currents, and their probability distribution functions have long tails of high concentration. The dilution rate is controlled by submesoscale stirring and straining in tracer filaments. The dominant dispersal pattern is alongshore in both directions, approximately along isobaths, over distances of more than 10. km before dilution takes over. The current outfall locations mostly keep the effluent below the surface and away from the shore, as intended, but the nearshore diversions do not. © 2014 Elsevier Ltd.

  14. Channel Responses and Hydromodification in Southern California

    Science.gov (United States)

    Hawley, R. J.; Dust, D. W.; Bledsoe, B. P.

    2007-12-01

    Hydromodification (changes in watershed hydrologic characteristics, and the resulting hydraulics and channel forms due to urbanization) is ubiquitous in Southern California. In this region, the effects of hydromodification are driven and compounded by the arid/semiarid climate, high relief, erodible soils, high urbanization rates, and relatively low frequency of retention/detention. We conducted a preliminary survey of over 50 stream reaches along a gradient from least disturbed to fully urbanized. All stages of the Channel Evolution Model (CEM) of Schumm et al. (1984) were observed, from stable to degrading, widening, aggrading, and quasi-equilibrium channels. Several sites have CEM stages II through V in close proximity due to headcutting, hardpoints, and infrastructure. We also observed channels in undeveloped watersheds impacted by downstream urbanization via headcutting. A range of intervention measures was observed, with the frequent evolutionary endpoint as a concrete engineered flood control channel. We also observed multiple channel evolution sequences that deviate from the CEM for single-thread, incising channels. An alternative channel response, particularly on smaller urbanized streams is a stabilized, vegetation encroached low-flow channel with regular baseflow supplied by residential irrigation runoff. The limited cases of unimpacted streams that remain tend to be high gradient, high energy systems that are naturally proximate to the transition between braided and meandering form for a given sediment size.

  15. UBV photometry of 262 southern galaxies

    International Nuclear Information System (INIS)

    Wegner, G.

    1979-01-01

    Multi-aperture photometry of 262 bright southern galaxies in the Johnson UBV system is given. Most of these are south of delta = -30 0 , although some northward to delta = -10 0 are included. A total of 169 objects have published radial velocity determinations. These provide distances, and enable construction of colour-magnitude diagrams for this subset of objects througha physical diameter of 2.0 kpc (with H 0 = 100). The two-colour diagrams for the inner regions of the galaxies differ from those of integrated galaxies due to the colour changes towards their centres. Comparison with theoretical models of Larson and Tinsley (1978) suggest that the colours of the inner portions of most ellipticals and lenticulars are consistent with their having all stars formed at nearly one epoch with little subsequent star formation, while for spirals larger amounts of star formation, either in bursts of continuously, are suggested. This simple picture is complicated by the presence of certain objects having peculiar colours indicative of large amounts of recent star formation. (Auth.)

  16. Holocene geologic slip rate for the Banning strand of the southern San Andreas Fault, southern California

    Science.gov (United States)

    Gold, Peter O.; Behr, Whitney M.; Rood, Dylan; Sharp, Warren D.; Rockwell, Thomas; Kendrick, Katherine J.; Salin, Aaron

    2015-01-01

    Northwest directed slip from the southern San Andreas Fault is transferred to the Mission Creek, Banning, and Garnet Hill fault strands in the northwestern Coachella Valley. How slip is partitioned between these three faults is critical to southern California seismic hazard estimates but is poorly understood. In this paper, we report the first slip rate measured for the Banning fault strand. We constrain the depositional age of an alluvial fan offset 25 ± 5 m from its source by the Banning strand to between 5.1 ± 0.4 ka (95% confidence interval (CI)) and 6.4 + 3.7/−2.1 ka (95% CI) using U-series dating of pedogenic carbonate clast coatings and 10Be cosmogenic nuclide exposure dating of surface clasts. We calculate a Holocene geologic slip rate for the Banning strand of 3.9 + 2.3/−1.6 mm/yr (median, 95% CI) to 4.9 + 1.0/−0.9 mm/yr (median, 95% CI). This rate represents only 25–35% of the total slip accommodated by this section of the southern San Andreas Fault, suggesting a model in which slip is less concentrated on the Banning strand than previously thought. In rejecting the possibility that the Banning strand is the dominant structure, our results highlight an even greater need for slip rate and paleoseismic measurements along faults in the northwestern Coachella Valley in order to test the validity of current earthquake hazard models. In addition, our comparison of ages measured with U-series and 10Be exposure dating demonstrates the importance of using multiple geochronometers when estimating the depositional age of alluvial landforms.

  17. The new southern novel: a bibliographical assay The new southern novel: a bibliographical assay

    OpenAIRE

    Robert F. Kiernan

    2008-01-01

    Any attempt to chronicle the contemporary southern novel is beset by troublesome, interlocking questions. Is the very existence of the genro presumptive? If not, does its tradition continue to instill a sectional awareness among writers flourishing in the last years of the twentieth century? If so, is it not the case that to read any literary text as a mimetic expression of a geographical region is to confine each to the other and to ignore the quiddity of both? no task of the chronicler...

  18. Fire Perimeters - Southern California, Fall 2007 [ds385

    Data.gov (United States)

    California Natural Resource Agency — Southern Callifornia fire perimeters for the Fall 2007 wildfires. The perimeters were assembled from various sources by California Department of Fish and Game (DFG)...

  19. Mammals of the Kammanassie Mountains, southern Cape Province

    African Journals Online (AJOL)

    regrettable as in the southern Cape, for example, the marked gradients in .... green plant material (leaves) and insects, using a stereo- scopic microscope with ..... cial and logistic support, especially Mr P. Ie Roux, the. Assistant Director, for his ...

  20. Prevalence of Risk Factors for Coronary Artery Disease in Southern ...

    African Journals Online (AJOL)

    (48.5 %), fatty food consumption (47.5 %), obesity (38 %) and smoking (37 %), respectively. Other less ... Keywords: Risk factors, Prevalence, Coronary artery disease, Diabetes, Southern Punjab ... developing world, including Pakistan [1]. The.

  1. Seasonal temperature prediction skill over Southern Africa and human health

    CSIR Research Space (South Africa)

    Lazenby, MJ

    2014-10-01

    Full Text Available An assessment of probabilistic prediction skill of seasonal temperature extremes over Southern Africa is presented. Verification results are presented for six run-on seasons; September to November, October to December, November to January, December...

  2. The beginnings of the Southern Child/Pediatric Neurology Society.

    Science.gov (United States)

    Dyken, Paul Richard; Bodensteiner, John B

    2015-04-01

    The founding and early development of the Southern Pediatric Neurology Society was in many ways parallel to that of the Child Neurology Society. The organization started out as the Southern Child Neurology Society but the name was changed at the time of incorporation so as to avoid confusion of identity and purpose with the larger Child Neurology Society. Although there are archives of early days and the later development of the Southern Pediatric Neurology Society, the details have never been set down in a narrative explaining the events that led to the development of the organization. In this paper, we try to produce a written record of the history of the founding and early development of the Southern Pediatric Neurology Society. © The Author(s) 2014.

  3. determination of the power law exponent for southern highlands

    African Journals Online (AJOL)

    Mgina

    site located in the southern highland zone of Tanzania, was established using wind speeds measured at heights ... distribution, turbulence and wind gusts at the ... mathematical models, which normally .... other locations that have similar wind.

  4. Southern African Journal of Environmental Education - Vol 8 (1988)

    African Journals Online (AJOL)

    Southern African Journal of Environmental Education - Vol 8 (1988) ... An interaction of archaeology with school history in a museum education context ... The child in the outdoor classroom · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  5. Model simulations of rainfall over southern Africa and its eastern ...

    African Journals Online (AJOL)

    2016-01-01

    Jan 1, 2016 ... Rainfall simulations over southern and tropical Africa in the form of low-resolution Atmospheric Model ..... provision of sea-surface temperatures and sea-ice fields of a host ...... with variability of the Atlantic Ocean. Bull.

  6. Research Ship Southern Surveyor Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Southern Surveyor Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  7. Population structure of Han nationality in Central-Southern China.

    Science.gov (United States)

    Liu, Qiu-Ling; Chen, Ye-Fei; He, Xin; Shi, Yan-Wei; Wu, Wei-Wei; Zhao, Hu; Lu, De-Jian

    2017-07-01

    Knowledge of population structure is very important for forensic genetics. However, the population substructure in Central-Southern China Han nationality has still not been fully described. In this study, we investigated the genetic diversity of 15 forensic autosomal STR loci from 6879 individuals in 12 Han populations subdivided by administrative provinces in Central-Southern China. The statistical analysis of genetic variation showed that genetic differentiation among these populations was very small with a F st value of 0.0009. The Discriminant Analysis of Principal Components (DAPC) showed that there were no obvious population clusters in Central-Southern China Han population. In practice, the population structure effect in Central-Southern China Han population can be negligible in forensic identification and paternity testing. Copyright © 2017. Published by Elsevier B.V.

  8. Phytoplankton composition and biomass across the southern Indian Ocean

    DEFF Research Database (Denmark)

    Schlüter, Louise; Henriksen, Peter; Nielsen, Torkel Gissel

    2011-01-01

    prochlorophytes dominated at these two stations, but also pelagophytes, haptophytes and cyanobacteria were abundant. Haptophytes Type 6 (sensu Zapata et al., 2004), most likely Emiliania huxleyi, and pelagophytes were the dominating eucaryotes in the southern Indian Ocean. Prochlorophytes dominated...

  9. Transboundary Water Resources in Southern Africa: Conflict or cooperation?

    CSIR Research Space (South Africa)

    Patrick, MJ

    2006-01-01

    Full Text Available Literature suggests a linkage between internationally shared water resources and conflict potential. Anthony R. Turton, Marian J. Patrick and Frederic Julien examine transboundary water resource management in southern Africa, showing that empirical...

  10. Southern Monterey Bay Littoral Cell CRSMP Sensitive Habitat 2008

    Data.gov (United States)

    California Natural Resource Agency — One of the most important functions of the southern Monterey Bay coastal system is its role as a habitat for a unique flora and fauna. The beaches are habitat for...

  11. Academic publishing: Lessons learnt from the Southern African ...

    African Journals Online (AJOL)

    Key words: academic publishing, peer review, Southern African Business Review ... Management Sciences of Unisa, for example, 16.5% of academics ..... as scientific field of manuscript; to number, origin and designation of authors; and.

  12. Straight studs from southern pine veneer cores and cordwood

    Science.gov (United States)

    Peter Koch

    1968-01-01

    An economically feasible system has been developed for converting southern pine veneer cores into straight 8-foot studs (2). Prototype studs - two per core - were 100 percent SPIB stud grade and better.

  13. Southern Monterey Bay Littoral Cell CRSMP Proposed Receiver Site 2008

    Data.gov (United States)

    California Natural Resource Agency — Given the location of the critical areas of erosion and the need to avoid adverse impacts to local sensitive habitat, the Southern Monterey Bay Coastal RSM Plan...

  14. Environmental education and quality of life | Bak | Southern African ...

    African Journals Online (AJOL)

    Southern African Journal of Environmental Education. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 17 (1997) >. Log in or Register to get access to full text downloads.

  15. Access to Knowledge Southern Africa : Universities, Open Research ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Journal articles. Research productivity-visibility-accessibility and scholarly communication in Southern African universities. Download PDF ... Call for new OWSD Fellowships for Early Career Women Scientists now open. In partnership with ...

  16. ESARBICA Journal: Journal of the Eastern and Southern Africa ...

    African Journals Online (AJOL)

    ... (IKS) in the Southern African Development Community (SADC): One step at a time ... The role of records management in promoting business efficiency and ... controversy: a case for records keeping in international sports management ...

  17. Promoting production and marketing of root crops in Southern Africa ...

    African Journals Online (AJOL)

    African Journal of Food, Agriculture, Nutrition and Development ... climate using integrated Agricultural Research for Development (IAR4d) pathway ... smallholder farmers in southern Africa continue to show preference to maize over other ...

  18. (MSV) resistant maize varieties for adaptation to a southern guinea

    African Journals Online (AJOL)

    STORAGESEVER

    2009-10-05

    Oct 5, 2009 ... African Journal of Biotechnology Vol. 8 (19), pp. ... the development of superior maize varieties for the southern guinea savanna ecology. Key words: Zea ... tional agricultural research systems have succeeded in converting ...

  19. Forests, rangelands and climate change in Southern Africa

    CSIR Research Space (South Africa)

    Naidoo, Sasha

    2013-09-01

    Full Text Available This paper provides an analysis of the implications of climate change for forests and rangelands in southern Africa. The extent of the resources and their economic and social functions and drivers of change is outlined. The vulnerability...

  20. A Critical Approach towards Jade Axes in Southern Scandinavia

    DEFF Research Database (Denmark)

    Sørensen, Lasse

    2013-01-01

    Southern Scandinavian jade axes have been interpreted as items of prestigious exchange illustrating contact with the agrarian societies of Central Europe and reflecting agrarian ideas and ideology. They are therefore important in the discussion concerning the process of neolithisation in Northern...... axes produced in local raw materials clearly indicate the importance of this artefact group within the Mesolithic and Neolithic transition in Southern Scandinavia....... Europe, but the difficulties in differentiating between Neolithic axes of alpine jade from axes imported from other continents has attracted some criticism. Furthermore, some of the jade axes found in Southern Scandinavian collections originate from private collectors, many of whom had contacts all over...... Europe. The axes lack therefore secure archaeological contexts, and may suggest that they have not been found in Scandinavian soil. The aim of this paper is to maintain a critical approach towards the question of the origin of the jade axes from Southern Scandinavia. However, the many imitations of jade...

  1. Assessment of Aero-radiometric Data of Southern Anambra Basin ...

    African Journals Online (AJOL)

    ADOWIE PERE

    Potassium and Thorium) were used and processed independently to investigate the Southern. Anambra basin for the .... Based on the Rybach equation (1) above, Uranium produces the .... Geothermal Exploration: State of the Art. Techniques.

  2. VT Green Mountain National Forest Map - Southern Section

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) The BasemapOther_GMNFMAPS is a cartographic map product depicting the southern half of the Green Mountain National Forest (GMNF). The paper map...

  3. Killer Whale Genetic Data - Southern resident killer whale pedigree analysis

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — In this project, we are using genetic variation to infer mating patterns in the southern killer whale community. In Canada, this population was listed as threatened...

  4. Patterns of maternity care service utilization in Southern Ethiopia ...

    African Journals Online (AJOL)

    Patterns of maternity care service utilization in Southern Ethiopia: Evidence from a community ... PROMOTING ACCESS TO AFRICAN RESEARCH ... Result: The study revealed that only 26.1 % and 3.3% of the women received antenatal and ...

  5. Southern Monterey Bay Littoral Cell CRSMP Proposed Receiver Site 2008

    Data.gov (United States)

    California Department of Resources — Given the location of the critical areas of erosion and the need to avoid adverse impacts to local sensitive habitat, the Southern Monterey Bay Coastal RSM Plan...

  6. Southern Monterey Bay Littoral Cell CRSMP Sensitive Habitat 2008

    Data.gov (United States)

    California Department of Resources — One of the most important functions of the southern Monterey Bay coastal system is its role as a habitat for a unique flora and fauna. The beaches are habitat for...

  7. systems, pelagic fish of the southern Benguela are dominated

    African Journals Online (AJOL)

    denise

    cyclopoid copepods, anchovy eggs and crustacean eggs being the primary prey types. ... Key words: feeding, phytoplankton, sardine, southern Benguela, zooplankton. * Marine ...... KAWASAKI, T. and A. KUMAGAI 1984 — Food habits of the.

  8. Southern African Business Review - Vol 21, No 1 (2017)

    African Journals Online (AJOL)

    Southern African Business Review - Vol 21, No 1 (2017) ... Pre-packaged applications in business reorganisations: International principles · EMAIL ... Microcredit supply under Islamic Banking in Khartoum State, Sudan · EMAIL FREE FULL ...

  9. Southern African Linguistics and Applied Language Studies - Vol 31 ...

    African Journals Online (AJOL)

    Southern African Linguistics and Applied Language Studies. ... Object marking restrictions on Shona causative and applicative constructions · EMAIL ... A problem-oriented, form-focused course design for teaching isiZulu as an additional ...

  10. JASSA: Journal of Applied Science in Southern Africa

    African Journals Online (AJOL)

    The Journal of Applied Science in Southern Africa (JASSA), aims to publish each issue six to ten original research papers. It is multi-disciplinary in approach specializing in publishing results of applied research considered important to the region.

  11. Towards a spatial development perspective for Southern Africa

    CSIR Research Space (South Africa)

    Chakwizira, J

    2008-11-01

    Full Text Available To ensure equitable development in the Southern African Development Community (SADC) region, unravelling and understanding the role that physical space and spatial structures play in economic growth is of cardinal importance. In this regard...

  12. PROSPECTS OF REGIONAL NETWORK STRUCTURES IN THE SOUTHERN FEDERAL DISTRICT

    Directory of Open Access Journals (Sweden)

    I. V. Morozov

    2014-01-01

    Full Text Available The article reveals the possibility of the Southern Federal District to form regional network structures. The prospects for the formation of networks in the region in relation to the Olympic Winter Games Sochi 2014.

  13. The Prevalence of Hepatitis B (Australia) Antigen in Southern Mrica

    African Journals Online (AJOL)

    Bushmen) or rural Blacks indicated that geographical environment might be one of the factors influencing antigenaemia in healthy persons. The prevalence was highest in persons originating from the west coast regions of Southern Africa, ...

  14. A nurse's perspective on the ART rollout | Tito | Southern African ...

    African Journals Online (AJOL)

    Southern African Journal of HIV Medicine. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 15, No 1 (2014) >. Log in or Register to get access to full text downloads.

  15. Reflections on six years in paediatric ART | Moore | Southern African ...

    African Journals Online (AJOL)

    Southern African Journal of HIV Medicine. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 15, No 1 (2014) >. Log in or Register to get access to full text downloads.

  16. The role of fine art in environmental education | Hardy | Southern ...

    African Journals Online (AJOL)

    Southern African Journal of Environmental Education. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 8 (1988) >. Log in or Register to get access to full text downloads.

  17. Antarctic stratospheric ozone and seasonal predictability over southern Africa

    CSIR Research Space (South Africa)

    Engelbrecht, FA

    2015-09-01

    Full Text Available of the Atmospheric Model Intercomparison project (AMIP) was performed first, generating different ensemble members using a lagged-average forecasting approach. These simulations are shown to be skilful in representing southern African summer-season inter...

  18. Book Reviews | Various Authors | Shakespeare in Southern Africa

    African Journals Online (AJOL)

    Shakespeare in Southern Africa. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 18 (2006) >. Log in or Register to get access to full text downloads.

  19. On Playing Cleopatra | Suzman | Shakespeare in Southern Africa

    African Journals Online (AJOL)

    Shakespeare in Southern Africa. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 25 (2013) >. Log in or Register to get access to full text downloads.

  20. Book Reviews | Various Authors | Shakespeare in Southern Africa

    African Journals Online (AJOL)

    Shakespeare in Southern Africa. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 19 (2007) >. Log in or Register to get access to full text downloads.

  1. Is Shylock a Catholic? | Jeffery | Shakespeare in Southern Africa

    African Journals Online (AJOL)

    Shakespeare in Southern Africa. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 16 (2004) >. Log in or Register to get access to full text downloads.

  2. Book reviews | Various Authors | Shakespeare in Southern Africa

    African Journals Online (AJOL)

    Shakespeare in Southern Africa. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 15 (2003) >. Log in or Register to get access to full text downloads.

  3. Hamlet the Populist Politician | Titlestad | Shakespeare in Southern ...

    African Journals Online (AJOL)

    Shakespeare in Southern Africa. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 25 (2013) >. Log in or Register to get access to full text downloads.

  4. In Search of Perfect Freedom | Houliston | Shakespeare in Southern ...

    African Journals Online (AJOL)

    Shakespeare in Southern Africa. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 26 (2014) >. Log in or Register to get access to full text downloads.

  5. Theatre Reviews | Various Authors | Shakespeare in Southern Africa

    African Journals Online (AJOL)

    Shakespeare in Southern Africa. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 14 (2002) >. Log in or Register to get access to full text downloads.

  6. A synopsis of Peristrophe (Acanthaceae in southern Africa

    Directory of Open Access Journals (Sweden)

    K. Balkwill

    1996-10-01

    Full Text Available A synopsis of Peristrophe Nees (Acanthaceae in southern Africa is provided. Peristrophe comprises nine species, one with two subspecies in southern Africa. A key for identification and descriptions of species not included in recent literature are provided. Morphology of tertiary bracts,indumentum on stems and distribution is illustrated and diagnostic characters, distribution, habitat, flowering  time and conservation status are discussed for each species.

  7. Thalassemia and Hemoglobin E in Southern Thai Blood Donors

    OpenAIRE

    Nuinoon, Manit; Kruachan, Kwanta; Sengking, Warachaya; Horpet, Dararat; Sungyuan, Ubol

    2014-01-01

    Thalassemia and hemoglobin E (Hb E) are common in Thailand. Individuals with thalassemia trait usually have a normal hemoglobin concentration or mild anemia. Therefore, thalassemic individuals who have minimum acceptable Hb level may be accepted as blood donors. This study was aimed at determining the frequency of α-thalassemia 1 trait, β-thalassemia trait, and Hb E-related syndromes in Southern Thai blood donors. One hundred and sixteen voluntary blood donors, Southern Thailand origin, were ...

  8. AMS radiocarbon dating of 'Grotta Cappuccini' in Southern Italy

    International Nuclear Information System (INIS)

    Quarta, G.; Calcagnile, L.; D'Elia, M.; Rizzo, A.; Ingravallo, E.

    2004-01-01

    We present the results of AMS radiocarbon dating of human bones recovered in 'Grotta Cappuccini', a prehistoric cave in Galatone, Lecce (Southern Italy). The AMS analysis has confirmed the archaeological dating of the cave to the period between the end of the Copper Age and the early Bronze Age, and has given a fundamental contribution to the chronological definition of an important cultural aspect of the prehistory of Southern Italy

  9. Distribution and phenology of ixodid ticks in southern Zambia.

    Science.gov (United States)

    Speybroeck, N; Madder, M; Van Den Bossche, P; Mtambo, J; Berkvens, N; Chaka, G; Mulumba, M; Brandt, J; Tirry, L; Berkvens, D

    2002-12-01

    Distribution data for epidemiologically important ticks (Acari: Ixodidae) in the Southern Province of Zambia, one of the main cattle areas of the country, are presented. Boophilus microplus (Canestrini) was not recorded in southern Zambia, whereas Boophilus decoloratus (Koch) is present throughout the area. New distribution patterns for less economically important ixodid ticks are also discussed. Southern Zambia is a transition zone because it is the most northern area in Africa where mixed Rhipicephalus appendiculatus Neumann and Rhipicephalus zambeziensis Walker, Norval & Corwin populations were reported. Although a second generation of adult R. appendiculatus/R. zamnbeziensis was encountered, simulations indicated that this phenomenon is very rare in southern Zambia, mainly because of the colder temperatures during the early dry season and lower rainfall. These simulations were supported by a development trial under experimental conditions. Tick body size measurements showed that southern Zambian ticks are larger than eastern Zambian R. appendiculatus. It is hypothesized that body size is related to diapausing intensity in this species. The epidemiological consequences are that a different approach to control Theileria parva (Theiler) (Piroplasmida: Theileriidae) and other tick-borne diseases is needed in southern Zambia, compared to the one adopted in eastern Zambia.

  10. Radioactive fallout in the southern hemisphere from nuclear weapons tests

    International Nuclear Information System (INIS)

    Moroney, J.R.

    1979-11-01

    Fallout in the southern hemisphere, and its origins in the national programs of atmospheric nuclear weapons testing in both hemispheres, are reviewed. Of the 390 nuclear tests conducted in the atmosphere to date, 53 were carried out in the southern hemisphere and it is the second phase of these, between 1966 and 1974, that is seen to have been responsible for the main fallout of short-lived fission products in the southern hemisphere. In contrast to this, the programs of atmospheric nuclear testing in the northern hemisphere up to 1962 are shown to have been the main source of long-lived fission products in fallout in the southern hemisphere. The course followed by this contamination through the environment of the southern hemisphere is traced for the national programs of nuclear testing after 1962 taken separately (France, China) and for the earlier national programs taken together (U.S.S.R., U.S.A. and U.K.). The impact on populations in the southern hemisphere of fallout from atmospheric nuclear weapons tests to date is assessed

  11. Radioactive fallout in the southern hemisphere from nuclear weapons tests

    International Nuclear Information System (INIS)

    Moroney, J.R.

    1979-01-01

    Fallout in the southern hemisphere, and its origins in the national programs of atmospheric nuclear weapons testing in both hemispheres, are reviewed. Of the 390 nuclear tests conducted in the atmosphere to date, 53 were carried out in the southern hemisphere and it is the second phase of these, between 1966 and 1974, that is seen to have been responsible for the main fallout of short-lived fission products in the southern hemisphere. In contrast to this, the programs of atmospheric nuclear testing in the northern hemisphere up to 1962 are shown to have been the main source of long-lived fission products in fallout in the southern hemisphere. The course followed by this contamination through the environment of the southern hemisphere is traced for the national programs of nuclear testing after 1962 taken separately (France, China) and for the earlier national programs taken together (U.S.S.R., U.S.A. and U.K.). The impact on populations in the southern hemisphere of fallout from atmospheric nuclear weapons tests to date is assessed. (author)

  12. Nearshore marine fish diversity in southern California using trawl information from the Southern California Coastal Water Research Project.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This is a point file of mean fish diversity within 5 minute grid cells. The Shannon Index of diversity was calculated from Southern California Coastal Water Research...

  13. The bi-decadal rainfall cycle, Southern Annular Mode and tropical cyclones over the Limpopo River Basin, southern Africa

    CSIR Research Space (South Africa)

    Malherbe, J

    2014-06-01

    Full Text Available contribution to rainfall by tropical cyclones and depressions. The findings suggest that a broadening of the Hadley circulation underpinned by an anomalous anticyclonic pattern to the east of southern Africa altered tropospheric steering flow, relative...

  14. Characteristics of Southern California coastal aquifer systems

    Science.gov (United States)

    Edwards, B.D.; Hanson, R.T.; Reichard, E.G.; Johnson, T.A.

    2009-01-01

    Most groundwater produced within coastal Southern California occurs within three main types of siliciclastic basins: (1) deep (>600 m), elongate basins of the Transverse Ranges Physiographic Province, where basin axes and related fluvial systems strike parallel to tectonic structure, (2) deep (>6000 m), broad basins of the Los Angeles and Orange County coastal plains in the northern part of the Peninsular Ranges Physiographic Province, where fluvial systems cut across tectonic structure at high angles, and (3) shallow (75-350 m), relatively narrow fluvial valleys of the generally mountainous southern part of the Peninsular Ranges Physiographic Province in San Diego County. Groundwater pumped for agricultural, industrial, municipal, and private use from coastal aquifers within these basins increased with population growth since the mid-1850s. Despite a significant influx of imported water into the region in recent times, groundwater, although reduced as a component of total consumption, still constitutes a significant component of water supply. Historically, overdraft from the aquifers has caused land surface subsidence, flow between water basins with related migration of groundwater contaminants, as well as seawater intrusion into many shallow coastal aquifers. Although these effects have impacted water quality, most basins, particularly those with deeper aquifer systems, meet or exceed state and national primary and secondary drinking water standards. Municipalities, academicians, and local water and governmental agencies have studied the stratigraphy of these basins intensely since the early 1900s with the goals of understanding and better managing the important groundwater resource. Lack of a coordinated effort, due in part to jurisdictional issues, combined with the application of lithostratigraphic correlation techniques (based primarily on well cuttings coupled with limited borehole geophysics) have produced an often confusing, and occasionally conflicting

  15. Cephalopods distribution in the southern Aegean Sea

    Directory of Open Access Journals (Sweden)

    E. LEFKADITOU

    2003-06-01

    Full Text Available The present study concerns faunal composition and distribution of cephalopods in the southern Aegean Sea (35Ζ 13’ 19’’- 37Ζ 55’ 25’’ N, 23Ζ 00’ 15’’ - 28Ζ 15’ 37’’ E. Samples were collected from 708 hauls, obtained by an experimental bottom trawl net during eight surveys carried out in the summers of the years 1994-2001, as well as by commercial trawl net during four surveys carried out in September 1995, December 1995, May and September 1996. The hauls were performed at depths ranging from 16 to 778 m. A total of 34 species of cephalopod in 12 families were identified, including 11 oegopsid squid, 3 myopsid squid, 7 octopod, 3 cuttlefish and 10 sepiolid. Trawling with the experimental net resulted in the capturing of some uncommon pelagic species, such as Ctenopteryx siculaand Octopoteuthis sicula, which were recorded for the first time in the Aegean Sea. Most of the species showed a wide depth and geographical range. The species: Sepia officinalis, Sepietta neglecta, Sepietta obscuraand Sepiola rondeletiwere caught only on the continental shelf, whereas the Ancistroteuthis lichtensteini, Bathypolypous sponsalis, Brachioteuthis riisei, Chiroteuthis veranyi, Ctenopteryx sicula, Heteroteuthis dispar, Histioteuthis reversa, Neorossia caroli and Pyroteuthis margaritiferawere found only on the slope. The rest of the species extended in both continental shelf and slope. The spatial distribution of different species groups is discussed in relation to the hydrology and topography of the study area and the species ecology.

  16. Evidence of nutrition transition in Southern Africa.

    Science.gov (United States)

    Nnyepi, Maria S; Gwisai, Namo; Lekgoa, Malebogo; Seru, Tumelo

    2015-11-01

    Nutrition transition is characterised by shift to highly refined diets high in fat, salt and caloric sweeteners and low in fibre in rapidly growing economies. Dietary shifts occur almost concurrently with demographic and epidemiologic shifts, urbanisation and industrialisation and together contribute to increased prevalence of nutrition related (NR)-non-communicable disease (NCR). The emergence of nutrition transition in Southern Africa countries (SAC) was examined using anthropometric, NCD prevalence, and food consumption data. The findings reveal growing prevalence of overweight and obesity (OWOB) across SAC, with national prevalence estimated between 30 and 60 % in all but two SAC. Overweight prevalence in excess of 60 % has been reported in some sub-population groups. Hypertension prevalence of at least 30 % has also been reported. Further, the prevalence of OWOB and hypertension in many SAC exceeds that of HIV and is often at par with stunting in children. NCD are equally serious public health problems as stunting and HIV. Collectively, NR-NCD explain 20-31 % of mortality for Botswana, South Africa, Swaziland, Mozambique and Zambia. At least 72 % of adults in SAC have fewer servings of fruit and vegetable servings daily than recommended. Additionally, adults in SAC do poorly in physical activity; 31-75 % do not exercise regularly. Not surprisingly, 15-40 % of adults in SAC have at least three risk factors of CVD. SAC are grappling with NR-NCD which threaten to surpass infectious diseases burden. SAC are at various levels in interventions for moving their populations to stage 5, but there is room for much improvement.

  17. Phenological Changes in the Southern Hemisphere

    Science.gov (United States)

    Chambers, Lynda E.; Altwegg, Res; Barbraud, Christophe; Barnard, Phoebe; Beaumont, Linda J.; Crawford, Robert J. M.; Durant, Joel M.; Hughes, Lesley; Keatley, Marie R.; Low, Matt; Morellato, Patricia C.; Poloczanska, Elvira S.; Ruoppolo, Valeria; Vanstreels, Ralph E. T.; Woehler, Eric J.; Wolfaardt, Anton C.

    2013-01-01

    Current evidence of phenological responses to recent climate change is substantially biased towards northern hemisphere temperate regions. Given regional differences in climate change, shifts in phenology will not be uniform across the globe, and conclusions drawn from temperate systems in the northern hemisphere might not be applicable to other regions on the planet. We conduct the largest meta-analysis to date of phenological drivers and trends among southern hemisphere species, assessing 1208 long-term datasets from 89 studies on 347 species. Data were mostly from Australasia (Australia and New Zealand), South America and the Antarctic/subantarctic, and focused primarily on plants and birds. This meta-analysis shows an advance in the timing of spring events (with a strong Australian data bias), although substantial differences in trends were apparent among taxonomic groups and regions. When only statistically significant trends were considered, 82% of terrestrial datasets and 42% of marine datasets demonstrated an advance in phenology. Temperature was most frequently identified as the primary driver of phenological changes; however, in many studies it was the only climate variable considered. When precipitation was examined, it often played a key role but, in contrast with temperature, the direction of phenological shifts in response to precipitation variation was difficult to predict a priori. We discuss how phenological information can inform the adaptive capacity of species, their resilience, and constraints on autonomous adaptation. We also highlight serious weaknesses in past and current data collection and analyses at large regional scales (with very few studies in the tropics or from Africa) and dramatic taxonomic biases. If accurate predictions regarding the general effects of climate change on the biology of organisms are to be made, data collection policies focussing on targeting data-deficient regions and taxa need to be financially and logistically

  18. Venomous snakebites in children in southern Croatia.

    Science.gov (United States)

    Karabuva, Svjetlana; Vrkić, Ivana; Brizić, Ivica; Ivić, Ivo; Lukšić, Boris

    2016-03-15

    This retrospective study represents observation of 160 children and adolescents aged up to 18 years that experienced venomous snakebites in southern Croatia and were treated in the Clinical Department of Infectious Diseases in the University Hospital Centre Split from 1979 to 2013. The main purpose of this research was to determine the epidemiological characteristics, clinical presentation, local and general complications, and received treatment. Most bites occurred during warm months, from early May to late August (80%), mostly in May and June. Upper limb bites were more frequent (59%) than lower limb bites (40%). Out of the total number of poisoned children, 24% developed local, and 25% general complications. The most common local complications were haemorrhagic blisters that occurred in 20% children, followed by compartment syndrome presented in 7.5% patients. The most dominated general complication was cranial nerve paresis or paralysis, which was identified in 11.2% patients, whereas shock symptoms were registrated in 7% children. According to severity of poisoning, 9.4% children had minor, 35% mild, 30.6% moderate, and 24.4% had severe clinical manifestation of envenomation. Only one (0.6%) child passed away because of snakebite directly on the neck. All patients received antivenom produced by the Institute of Immunology in Zagreb, tetanus prophylaxis as well, and almost all of them received antibiotics, and a great majority of them also received corticosteroids and antihistamines. Neighter anaphylactic reaction nor serum disease were noticed in our patients after administrating antivenom. A total of 26% children underwent surgical interventions, and incision of haemorrhagic blister was the most common applied surgical treatment, which was preformed in 15.6% patients, while fasciotomy was done in 7.5% subjects. All of our surgically treated patients recovered successfully. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. URBAN WATERSHED STUDIES IN SOUTHERN BRAZIL

    Directory of Open Access Journals (Sweden)

    Cristiano Poleto

    2007-12-01

    Full Text Available One of the greatest problems observed in Brazilian urban watersheds are concerned to the amount of solid residues, domestic sewerage and sediments that are disposed in the rivers and streams that drain those areas. This project aims to present these problems through a study of case taken in an urban watershed in Porto Alegre city, Southern Brazil. For this study, different procedures were used, such as field surveys, interviews with the inhabitants, satellite images, sediment samples, flow measures and morphology assessment of part of the local fluvial system to check the degree of instability of the channel. In 2005, it was verified that 42.57% of the watershed was impermeable, considering the paved streets, the residential and commercial buildings and stone pavements. As there was no sewer treatment, most of this sewerage was directly disposed into the stream and the TOC has reached 20% (m/m. Moreover, the occupation of riparian areas, a great amount of soil exposed in the watershed, the nonpaved streets and a great volume of solid residues were causing the instability in the channel, silting the stream bed. The metals (Zn, Pb and Cr selected for this study are most frequently found in high concentrations in urban areas. The results suggest the occurrence of a high enrichment of the fluvial sediment by these metals. The concentrations of these elements vary temporally during storms due to the input of impervious area runoff containing high concentration of elements associated to vehicular traffic and other anthropogenic activities. Then, it is possible to conclude that the contamination of the urban watershed is reflected in the results obtained in the fluvial suspended sediments.

  20. URBAN WATERSHED STUDIES IN SOUTHERN BRAZIL

    Directory of Open Access Journals (Sweden)

    Cristiano Poleto

    2007-01-01

    Full Text Available One of the greatest problems observed in Brazilian urban watersheds are concerned to the amount of solid residues, domestic sewerage and sediments that are disposed in the rivers and streams that drain those areas. This project aims to present these problems through a study of case taken in an urban watershed in Porto Alegre city, Southern Brazil. For this study, different procedures were used, such as field surveys, interviews with the inhabitants, satellite images, sediment samples, flow measures and morphology assessment of part of the local fluvial system to check the degree of instability of the channel. In 2005, it was verified that 42.57% of the watershed was impermeable, considering the paved streets, the residential and commercial buildings and stone pavements. As there was no sewer treatment, most of this sewerage was directly disposed into the stream and the TOC has reached 20% (m/m. Moreover, the occupation of riparian areas, a great amount of soil exposed in the watershed, the nonpaved streets and a great volume of solid residues were causing the instability in the channel, silting the stream bed. The metals (Zn, Pb and Cr selected for this study are most frequently found in high concentrations in urban areas. The results suggest the occurrence of a high enrichment of the fluvial sediment by these metals. The concentrations of these elements vary temporally during storms due to the input of impervious area runoff containing high concentration of elements associated to vehicular traffic and other anthropogenic activities. Then, it is possible to conclude that the contamination of the urban watershed is reflected in the results obtained in the fluvial suspended sediments.

  1. The Southern African Large Telescope project

    Science.gov (United States)

    Buckley, David A. H.; Charles, Philip A.; Nordsieck, Kenneth H.; O'Donoghue, Darragh

    The recently completed Southern African Large Telescope (SALT) is a low cost, innovative, 10 m class optical telescope, which began limited scientific operations in August 2005, just 5 years after ground-breaking. This paper describes the design and construction of SALT, including the first-light instruments, SALTICAM and the Robert Stobie Spectrograph (RSS). A rigorous systems engineering approach has ensured that SALT was built to specification, on budget, close to the original schedule and using a relatively small project team. The design trade-offs, which include an active spherical primary mirror array and a fixed altitude telescope with a prime focus tracker, although restrictive in comparison to conventional telescopes, have resulted in an affordable 10 m class telescope for South Africa and its ten partners. Coupled with an initial set of two seeing-limited instruments that concentrate on the UV-visible region (320 - 900 nm) and featuring some niche observational capabilities, SALT will have an ability to conduct some unique science. This includes high time resolution studies, for which some initial results have already been obtained. Many of the versatile modes available with the RSS - which is currently being commissioned - are unique and provide unparallelled opportunities for imaging polarimetry and spectropolarimetry. Likewise, Multi-Object Spectroscopy (with slit masks) and imaging spectroscopy with the RSS, the latter using Fabry-Perot étalons and interference filters, will extend the multiplex advantage over resolutions from 300 to 9000 and fields of view of 2 to 8 arcminutes. Future instrumentation plans include an extremely stable, fibre-fed, high resolution échelle spectrograph and a near-IR (to between 1.5 to 1.7 μm) extension to the RSS. Future development possibilities include phasing the primary mirror and AO. Finally, extrapolations of the SALT/HET designs to ELT proportions remain viable and are surely more affordable than conventional

  2. Completion of the Southern African Large Telescope

    Science.gov (United States)

    Buckley, D. A. H.; Charles, P. A.; O'Donoghue, D.; Nordsieck, K. H.

    2006-08-01

    The Southern African Large Telescope (SALT) is a low cost (19.7M), innovative, 10-m class optical telescope, which was inaugurated on 10 November 2005, just 5 years after ground-breaking. SALT and its first-light instruments are currently being commissioned, and full science operations are expected to begin later this year. This paper describes the design and construction of SALT, including the first-light instruments, SALTICAM and the Robert Stobie Spectrograph (RSS). A rigorous Systems Engineering approach was adopted to ensure that SALT was built to specification, on budget, close to the original schedule and using a relatively small project team. The design trade-offs, which include an active spherical primary mirror array in a fixed altitude telescope with a prime focus tracker, although restrictive in comparison to conventional telescopes, have resulted in an affordable and capable 10-m class telescope for South Africa and its ten partners. Coupled with an initial set of two seeing-limited instruments that concentrate on the UV-visible region (320 - 900nm) and featuring some unique observational capabilities, SALT will have an ability to conduct a wide range of science programs. These will include high time resolution studies, for which some initial results have already been obtained and are presented here. Many of the versatile modes available with the RSS will provide unparalleled opportunities for imaging polarimetry and spectropolarimetry. Likewise, Multi-Object Spectroscopy (using laser cut graphite slit masks) and imaging spectroscopy with the RSS, the latter using Fabry-Perot etalons and interference filters, will extend the multiplex advantage over resolutions from R = 300 to 9000 over fields of view of 2 to 8 arcminutes. Future instrumentation plans include an extremely stable, fibre-fed, high resolution échelle spectrograph and a near-IR (possibly to 1.7 μm) extension to the RSS. Future development possibilities include phasing the primary mirror

  3. Preliminary technology report for Southern Sector bioremediation

    International Nuclear Information System (INIS)

    Brigmon, R.L.; White, R.; Hazen, T.C.; Jones, D.; Berry, C.

    1997-01-01

    This project was designed to demonstrate the potential of intrinsic bioremediation and phytoremediation in the Southern Sector of the A/M-Area at the Savannah River Site. A subsurface plume of trichloroethylene (TCE) and perchloroethylene (PCE) is present in the Lost Lake aquifer upgradient of the study site and is predicted to impact the area at some point in the future. The surface area along the Lost lake aquifer seep line where the plume is estimated to emerge was identified. Ten sites along the seep line were selected for biological, chemical, and contaminant treatability analyses. A survey was undertaken in this area to to quantify the microbial and plant population known to be capable of remediating TCE and PCE. The current groundwater quality upgradient and downgradient of the zone of influence was determined. No TCE or PCE was found in the soils or surface water from the area tested at this time. A TCE biodegradation treatability test was done on soil from the 10 selected locations. From an initial exposure of 25 ppm of TCE, eight of the samples biodegraded up to 99.9 percent of all the compound within 6 weeks. This biodegradation of TCE appears to be combination of aerobic and anaerobic microbial activity as intermediates that were detected in the treatability test include vinyl chloride (VC) and the dichloroethenes (DCE) 1,2-cis-dichloroethylene and 1,1-dichloroethylene. The TCE biological treatability studies were combines with microbiological and chemical analyses. The soils were found through immunological analysis with direct fluorescent antibodies (DFA) and microbiological analysis with direct fluorescent antibodies (DFA) and microbiological analysis to have a microbial population of methanotrophic bacteria that utilize the enzyme methane monooxygenase (MMO) and cometabolize TCE

  4. Phenological changes in the southern hemisphere.

    Directory of Open Access Journals (Sweden)

    Lynda E Chambers

    Full Text Available Current evidence of phenological responses to recent climate change is substantially biased towards northern hemisphere temperate regions. Given regional differences in climate change, shifts in phenology will not be uniform across the globe, and conclusions drawn from temperate systems in the northern hemisphere might not be applicable to other regions on the planet. We conduct the largest meta-analysis to date of phenological drivers and trends among southern hemisphere species, assessing 1208 long-term datasets from 89 studies on 347 species. Data were mostly from Australasia (Australia and New Zealand, South America and the Antarctic/subantarctic, and focused primarily on plants and birds. This meta-analysis shows an advance in the timing of spring events (with a strong Australian data bias, although substantial differences in trends were apparent among taxonomic groups and regions. When only statistically significant trends were considered, 82% of terrestrial datasets and 42% of marine datasets demonstrated an advance in phenology. Temperature was most frequently identified as the primary driver of phenological changes; however, in many studies it was the only climate variable considered. When precipitation was examined, it often played a key role but, in contrast with temperature, the direction of phenological shifts in response to precipitation variation was difficult to predict a priori. We discuss how phenological information can inform the adaptive capacity of species, their resilience, and constraints on autonomous adaptation. We also highlight serious weaknesses in past and current data collection and analyses at large regional scales (with very few studies in the tropics or from Africa and dramatic taxonomic biases. If accurate predictions regarding the general effects of climate change on the biology of organisms are to be made, data collection policies focussing on targeting data-deficient regions and taxa need to be financially

  5. Southern pine beetle infestations in relation to forest stand conditions, previous thinning, and prescribed burning: evaluation of the Southern Pine Beetle Prevention Program

    Science.gov (United States)

    John T. Nowak; James R. Meeker; David R. Coyle; Chris A. Steiner; Cavell Brownie

    2015-01-01

    Since 2003, the Southern Pine Beetle Prevention Program (SPBPP) (a joint effort of the USDA Forest Service and Southern Group of State Foresters) has encouraged and provided cost-share assistance for silvicultural treatments to reduce stand/forest susceptibility to the southern pine beetle (SPB)(Dendroctonus frontalis Zimmermann) in the southeastern United States....

  6. Exotic Members of Southern Alaska's Jurassic Arc

    Science.gov (United States)

    Todd, E.; Jones, J. V., III; Karl, S. M.; Box, S.; Haeussler, P. J.

    2017-12-01

    The Jurassic Talkeetna arc and contemporaneous plutonic rocks of the Alaska-Aleutian Range batholith (ARB) are key components of the Peninsular terrane of southern Alaska. The Talkeetna arc, considered to be a type example of an intra-oceanic arc, was progressively accreted to northwestern North America in the Jurassic to Late Cretaceous, together with associated components of the Wrangellia Composite terrane. Older Paleozoic and Mesozoic rock successions closely associated with the ARB suggest that at least part of the Peninsular terrane might be an overlap succession built on pre-existing crust, possibly correlative with the Wrangellia terrane to the east. However, the relationship between the Talkeetna arc, ARB, and any pre-existing crust remains incompletely understood. Field investigations focused on the petrogenesis of the ARB near Lake Clark National Park show that Jurassic to Late Cretaceous plutonic rocks commonly host a diverse range of mineralogically distinct xenolith inclusions, ranging in size from several cm to hundreds of meters. The modal fraction of these inclusions ranges from 50% in some outcrops. They are generally mafic in composition and, with few exceptions, are more mafic than host plutonic rocks, although they are observed as both igneous (e.g., gabbro cumulate, diorite porphyry) and metamorphic types (e.g., amphibolite, gneiss and quartzite). Inclusion shapes range from angular to rounded with sharp to diffuse boundaries and, in some instances, are found as planar, compositionally distinct bands or screens containing high-temperature ductile shear fabrics. Other planar bands are more segmented, consistent with lower-temperature brittle behavior. Comparison of age, geochemical fractionation trends, and isotope systematics between the inclusions and host plutons provides a critical test of whether they are co-genetic with host plutons. Where they are related, mafic inclusions provide clues about magmatic evolution and fractionation history

  7. Southern Nevada Alternative Fuels Demonstration Project

    Energy Technology Data Exchange (ETDEWEB)

    Hyde, Dan; Fast, Matthew

    2009-12-31

    The Southern Nevada Alternative Fuels Program is designed to demonstrate, in a day-to-day bus operation, the reliability and efficiency of a hydrogen bus operation under extreme conditions. By using ICE technology and utilizing a virtually emission free fuel, benefits to be derived include air quality enhancement and vehicle performance improvements from domestically produced, renewable energy sources. The project objective is to help both Ford and the City demonstrate and evaluate the performance characteristics of the E-450 H2ICE shuttle buses developed by Ford, which use a 6.8-liter supercharged Triton V-10 engine with a hydrogen storage system equivalent to 29 gallons of gasoline. The technology used during the demonstration project in the Ford buses is a modified internal combustion engine that allows the vehicles to run on 100% hydrogen fuel. Hydrogen gives a more thorough fuel burn which results in more power and responsiveness and less pollution. The resultant emissions from the tailpipe are 2010 Phase II compliant with NO after treatment. The City will lease two of these E-450 H2ICE buses from Ford for two years. The buses are outfitted with additional equipment used to gather information needed for the evaluation. Performance, reliability, safety, efficiency, and rider comments data will be collected. The method of data collection will be both electronically and manually. Emissions readings were not obtained during the project. The City planned to measure the vehicle exhaust with an emissions analyzer machine but discovered the bus emission levels were below the capability of their machine. Passenger comments were solicited on the survey cards. The majority of comments were favorable. The controllable issues encountered during this demonstration project were mainly due to the size of the hydrogen fuel tanks at the site and the amount of fuel that could be dispensed during a specified period of time. The uncontrollable issues encountered during this

  8. A biogeographical study on tropical flora of southern China.

    Science.gov (United States)

    Zhu, Hua

    2017-12-01

    The tropical climate in China exists in southeastern Xizang (Tibet), southwestern to southeastern Yunnan, southwestern Guangxi, southern Guangdon, southern Taiwan, and Hainan, and these southern Chinese areas contain tropical floras. I checked and synonymized native seed plants from these tropical areas in China and recognized 12,844 species of seed plants included in 2,181 genera and 227 families. In the tropical flora of southern China, the families are mainly distributed in tropical areas and extend into temperate zones and contribute to the majority of the taxa present. The genera with tropical distributions also make up the most of the total flora. In terms of geographical elements, the genera with tropical Asian distribution constitute the highest proportion, which implies tropical Asian or Indo-Malaysia affinity. Floristic composition and geographical elements are conspicuous from region to region due to different geological history and ecological environments, although floristic similarities from these regions are more than 90% and 64% at the family and generic levels, respectively, but lower than 50% at specific level. These differences in the regional floras could be influenced by historical events associated with the uplift of the Himalayas, such as the southeastward extrusion of the Indochina geoblock, clockwise rotation and southeastward movement of Lanping-Simao geoblock, and southeastward movement of Hainan Island. The similarity coefficients between the flora of southern China and those of Indochina countries are more than 96% and 80% at family and generic levels, indicating their close floristic affinity and inclusion in the same biogeographically floristic unit.

  9. The unusual wet summer (July) of 2014 in Southern Europe

    Science.gov (United States)

    Ratna, Satyaban B.; Ratnam, J. V.; Behera, Swadhin K.; Cherchi, Annalisa; Wang, Wanqiu; Yamagata, Toshio

    2017-06-01

    Southern Europe (Italy and the surrounding countries) experienced an unusual wet summer in 2014. The monthly rainfall in July 2014 was 84% above (more than three standard deviation) normal with respect to the 1982-2013 July climatology. The heavy rainfall damaged agriculture, and affected tourism and overall economy of the region. In this study, we tried to understand the physical mechanisms responsible for such abnormal weather by using model and observed datasets. The anomalously high precipitation over Italy is found to be associated with the positive sea surface temperature (SST) and convective anomalies in the tropical Pacific through the atmospheric teleconnection. Rossby wave activity flux at upper levels shows an anomalous tropospheric quasi-stationary Rossby wave from the Pacific with an anomalous cyclonic phase over southern Europe. This anomalous cyclonic circulation is barotropic in nature and seen extending to lower atmospheric levels, weakening the seasonal high and causing heavy precipitation over the Southern Europe. The hypothesis is verified using the National Centers for Environmental Prediction (NCEP) coupled forecast system model (CFSv2) seasonal forecasts. It is found that two-month lead forecast of CFSv2 was able to capture the wet summer event of 2014 over Southern Europe. The teleconnection pattern from Pacific to Southern Europe was also forecasted realistically by the CFSv2 system.

  10. Sociology with a Southern Face: Why Are We Sociologists and What Are We Doing about It in the South?

    Science.gov (United States)

    Wimberley, Ronald C.

    2008-01-01

    Sociology with a Southern face characterizes Southern sociologists and Southern social issues. This article asks why we are sociologists and what we are doing about it in the South. I posit that many Southern sociologists share common biographical experiences that provide insights for understanding and solving Southern problems. Longstanding…

  11. Energy security in South America and Southern Africa: synthesis report

    International Nuclear Information System (INIS)

    Kiratu, Sheila

    2011-01-01

    In developing countries, the ability to provide adequate and reliable energy supplies is a key to sustainable economic development. The aim of this report is to study the energy supply security in both Southern Africa and South America and how these regions can participate in the international effort to combat climate change. This report showed that South Africa's energy sector mostly relies on carbon intensive coal while Brazil is mainly supplied by hydroelectricity. It was found that in both countries energy needs will increase significantly due to rising demand both internally and at a regional scale. However it was also shown that both Southern Africa and South America have important hydro, solar and wind renewable resources which could enhance their electricity security while minimizing their environmental impacts. This study demonstrated that Southern Africa and South America can enhance their electricity security through the use of renewable energies but that technology and financing is needed to develop the sector.

  12. Forecasting sea fog on the coast of southern China

    Science.gov (United States)

    Huang, H.; Huang, B.; Liu, C.; Tu, J.; Wen, G.; Mao, W.

    2016-12-01

    Forecast sea fog is still full of challenges. We have performed the numerical forecasting of sea fog on the coast of southern China by using the operational meso-scale regional model GRAPES (Global/Regional assimilation and prediction system). The GRAPES model horizontal resolution was 3km and with 66 vertical levels. A total of 72 hours forecasting of sea fog was conducted with hourly outputs over the sea fog event. The results show that the model system can predict reasonable characteristics of typical sea fog events on the coast of southern China. The scope of sea fog coincides with the observations of meteorological stations, the observations of the Marine Meteorological Science Experiment Base (MMSEB) at Bohe, Maoming and satellite products of sea fog. The goal of this study is to establish an operational numerical forecasting model system of sea fog on the coast of southern China.

  13. Recent changes in the ventilation of the southern oceans.

    Science.gov (United States)

    Waugh, Darryn W; Primeau, Francois; Devries, Tim; Holzer, Mark

    2013-02-01

    Surface westerly winds in the Southern Hemisphere have intensified over the past few decades, primarily in response to the formation of the Antarctic ozone hole, and there is intense debate on the impact of this on the ocean's circulation and uptake and redistribution of atmospheric gases. We used measurements of chlorofluorocarbon-12 (CFC-12) made in the southern oceans in the early 1990s and mid- to late 2000s to examine changes in ocean ventilation. Our analysis of the CFC-12 data reveals a decrease in the age of subtropical subantarctic mode waters and an increase in the age of circumpolar deep waters, suggesting that the formation of the Antarctic ozone hole has caused large-scale coherent changes in the ventilation of the southern oceans.

  14. Comparison of 'electrojet' indices from the northern and southern hemispheres

    International Nuclear Information System (INIS)

    Maclennan, C.G.; Lanzerotti, L.J.; Akasofu, S. I.; Zaitzev, A.N.; Popov, V.; Wilkinson, P.J.; Wolfe, A.

    1991-01-01

    A unique data set of digital and digitized analog magnetic recordings from 22 stations in the Antarctic was used to construct the southern hemisphere equivalent of the northern hemisphere auroral electrojet index, AE, for two separate intervals of magnetic disturbance (totaling seven days) in June 1982. A second index constructed using only 9 stations between ∼ 60 degrees-70 degrees geomagnetic south latitude showed only small differences from that using all 22 stations. For the universal time interval (00-11 UT inclusive) when a reasonably good coverage of ground stations exists in the austral auroral zone, it is found that there is a good correlation between the northern and southern hemisphere indices; this is the case even though the southern ionosphere is largely in total darkness during the interval studied. No effect of the north-south direction of the interplanetary magnetic field is found on the correlation

  15. Biodiversity of macrozoobenthos some running waters of southern Moravia

    Directory of Open Access Journals (Sweden)

    Ivo Sukop

    2010-01-01

    Full Text Available The present work gives the results of the research of macrozoobenthos some running waters drai­na­ge areas of the Dyje River (southern Moravia – Czech Republic. Altogether, 762 taxa of macrozoobenthos were determined from the running waters of southern Moravia. Porifera (3, Hydrozoa (3, Turbellaria (8, Nematoda (14, Nematomorpha (1, Oligochaeta (60, Hirudinea (18, Bryozoa (5, Mollusca (44, Isopoda (2, Amphipoda (4, Decapoda (2, Hydracarina (17, Ephemeroptera (65, Plecoptera (55, Odonata (26, Heteroptera (3, Plannipennia (2, Trichoptera (128, Coleoptera (59, Diptera (243. Some taxa of macrozoobenthos are extinct unfortunately in running waters of Southern Moravia at present time. Another ones appear newly, for example snail Potamopyrgus antipodarum from New Zealand or Dreissena polymorpha from Pontic region. The data presented in this paper may serve as a basis for future monitoring of water quality and zoobenthos composition in connection with presumption of climate changes.

  16. Southern Appalachian assessment. Summary report, Report 1 of 5

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-07-01

    This final report for the Southern Appalachian Man and the Biosphere Program is comprised of two documents: (1) a brief summary of programs and projects, and (2) a more extensive summary report included as an attachment. The purpose of the program is to promote a sustainable balance between the conservation of biological diversity, compatible economic uses, and cultural values across the Southern Appalachians. Program and project areas addressing regional issues include environmental monitoring and assessment, sustainable development/sustainable technologies, conservation biology, ecosystem management, environmental education and training, cultural and historical resources, and public information and education. The attached summary report is one of five that documents the results of the Southern Appalachian Assessment; it includes atmospheric, social/cultural/economic, terrestrial, and aquatic reports.

  17. Organizational Model of the Southern Asia Cluster Family Businesses

    Directory of Open Access Journals (Sweden)

    Vipin Gupta

    2013-07-01

    Full Text Available Recently, there has been an increased interest in the family business organization. Traditionally, the ideal typical organizational model was one where the management, governance, and ownership entities are kept separate. This principal agent model has been a subject of public debate in the wake of several corporate scandals. In the family business organization, significant management, governance and ownership is often with the members of a family & its trusted partners. It is common in the US to regulate the management, governance, and ownership roles of the family members by using competitive criteria for the involvement of different members. In Southern Asia cluster (Gupta & Hanges, 2004, on the other hand, it is quite common for the family involvement to be holistic and undivided, where the family collectively owns the shares in the family business. In this work, this organizational model of the Southern Asian family businesses is investigated. Keywords: Southern Asia, family business, organizational model

  18. Paleolakes in the Gobi region of southern Mongolia

    Science.gov (United States)

    Lehmkuhl, Frank; Grunert, Jörg; Hülle, Daniela; Batkhishig, Ochirbat; Stauch, Georg

    2018-01-01

    Numerous lakes and remnants of paleolakes exist in western and southern Mongolia. For six basins in the area, detailed geomorphological maps were compiled, based on extensive field studies and remote sensing datasets. Several phases of high and low lake levels were reconstructed and dated by radiocarbon and optically stimulated luminescence. During the marine isotope stage (MIS) 6 lakes in southern and western Mongolia mostly disappeared. In contrast, large paleolakes existed during the last interglacial (MIS 5e) and lasted probably until the beginning of the last glacial. These huge lakes were caused by a strong East Asian summer monsoon, which reached southern and even western Mongolia. During the MIS 3 the monsoon was considerably weaker and most of the lakes were relatively small or even disappeared. Higher lake levels of this period were only recorded at the Orog Nuur. However, at this time the lake was fed by glacial melt water from the Khangai Mountains. The MIS 2 was again a very dry period. The previously supposed phase of synchronous high lake levels and glaciations in southern and western Mongolia is not supported by the data presented here. During the Holocene, lakes in the western and southern part of the study area evolved differently. Early Holocene high lake levels were reconstructed for the western lakes, while most of the southern lakes had highest lake levels in the mid-Holocene. These differences can be attributed to different moisture bearing atmospheric systems. In the late Holocene lake levels were generally low and in the last 50 years most lakes completely disappeared due to a strong human usage of the water resources.

  19. Lessons learned by southern states in transportation of radioactive materials

    International Nuclear Information System (INIS)

    1992-03-01

    This report has been prepared under a cooperative agreement with DOE's Office of Civilian Radioactive Waste Management (OCRWM) and is a summary of the lessons learned by southern states regarding the transportation of radioactive materials including High-Level Radioactive Wastes (HLRW) and Spent Nuclear Fuel (SNF). Sources used in this publication include interviews of state radiological health and public safety officials that are members of the Southern States Energy Board (SSEB) Advisory Committee on Radioactive Materials Transportation, as well as the Board's Transuranic (TRU) Waste Transportation Working Group. Other sources include letters written by the above mentioned committees concerning various aspects of DOE shipment campaigns

  20. A synopsis of the tribe Desmodieae (Fabaceae in southern Africa

    Directory of Open Access Journals (Sweden)

    B. D. Schrire

    1988-10-01

    Full Text Available The tribe Desmodieae has a pantropical distribution and is one of the most advanced tribes in the subfamily Papilionoideae. Its greatest centres of development are in tropical Asia and America. Africa is relatively poorly endowed and only four genera comprising 16 species occur in the flora of southern Africa. Many of these species are widespread in the Old World tropics and the few African endemics appear to be closely related to them. A synopsis of the genera Desmodium, Pseudarthria, Alysicarpus and Lespedeza is given for southern Africa.