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Sample records for soluble sugars decreased

  1. Solubility and physical properties of sugars in pressurized water

    International Nuclear Information System (INIS)

    Saldaña, Marleny D.A.; Alvarez, Víctor H.; Haldar, Anupam

    2012-01-01

    Highlights: ► Sugar solubility in pressurized water and density at high pressures were measured. ► Glucose solubility was higher than that of lactose as predicted by their σ-profiles. ► Sugar aqueous solubility decreased with an increase in pressure from 15 to 120 bar. ► Aqueous glucose molecular packing shows high sensitivity to pressure. ► The COSMO-SAC model qualitatively predicted the sugar solubility data. - Abstract: In this study, the solubility, density, and refractive index of glucose and lactose in water as a function of temperature were measured. For solubility of sugars in pressurized water, experimental data were obtained at pressures of (15 to 120) bar and temperatures of (373 to 433) K using a dynamic flow high pressure system. Density data for aqueous sugar solutions were obtained at pressures of (1 to 300) bar and temperatures of (298 to 343) K. The refractive index of aqueous sugar solutions was obtained at 293 K and atmospheric pressure. Activity coefficient models, Van Laar and the Conductor-like Screening Model-Segment Activity Coefficient (COSMO-SAC), were used to fit and predict the experimental solubility data, respectively. The results obtained showed that the solubility of both sugars in pressurized water increase with an increase in temperature. However, with the increase of pressure from 15 bar to 120 bar, the solubility of both sugars in pressurized water decreased. The Van Laar model fit the experimental aqueous solubility data with deviations lower than 13 and 53% for glucose and lactose, respectively. The COSMO-SAC model predicted qualitatively the aqueous solubility of these sugars.

  2. Inter-species comparative analysis of components of soluble sugar concentration in fleshy fruits

    Directory of Open Access Journals (Sweden)

    Zhanwu eDai

    2016-05-01

    Full Text Available The soluble sugar concentration of fleshy fruit is a key determinant of fleshy fruit quality. It affects directly the sweetness of fresh fruits and indirectly the properties of processed products (e.g. alcohol content in wine. Despite considerable divergence among species, soluble sugar accumulation in a fruit results from the complex interplay of three main processes, namely sugar import, sugar metabolism, and water dilution. Therefore, inter-species comparison would help to identify common and/or species-specific modes of regulation in sugar accumulation. For this purpose, a process-based mathematical framework was used to compare soluble sugar accumulation in three fruits: grape, tomato and peach. Representative datasets covering the time course of sugar accumulation during fruit development were collected. They encompassed 104 combinations of species (3, genotypes (32, and growing conditions (19 years and 16 nutrient and environmental treatments. At maturity, grape showed the highest soluble sugar concentrations (16.5-26.3 g /100 g FW, followed by peach (2.2 to 20 g /100 g FW and tomato (1.4 to 5 g /100 g FW. Main processes determining soluble sugar concentration were decomposed into sugar importation, metabolism and water dilution with the process-based analysis. Different regulation modes of soluble sugar concentration were then identified, showing either import-based, dilution-based, or import and dilution dual-based. Firstly, the higher soluble sugar concentration in grape than in tomato is a result of higher sugar importation. Secondly, the higher soluble sugar concentration in grape than in peach is due to a lower water dilution. The third mode of regulation is more complicated than the first two, with differences both in sugar importation and water dilution (grape vs cherry tomato; cherry tomato vs peach; peach vs tomato. On the other hand, carbon utilization for synthesis of non-soluble sugar compounds (namely metabolism was

  3. Transcription Factor AREB2 Is Involved in Soluble Sugar Accumulation by Activating Sugar Transporter and Amylase Genes.

    Science.gov (United States)

    Ma, Qi-Jun; Sun, Mei-Hong; Lu, Jing; Liu, Ya-Jing; Hu, Da-Gang; Hao, Yu-Jin

    2017-08-01

    Sugars play important roles in plant growth and development, crop yield and quality, as well as responses to abiotic stresses. Abscisic acid (ABA) is a multifunctional hormone. However, the exact mechanism by which ABA regulates sugar accumulation is largely unknown in plants. Here, we tested the expression profile of several sugar transporter and amylase genes in response to ABA treatment. MdSUT2 and MdAREB2 were isolated and genetically transformed into apple ( Malus domestica ) to investigate their roles in ABA-induced sugar accumulation. The MdAREB2 transcription factor was found to bind to the promoters of the sugar transporter and amylase genes and activate their expression. Both MdAREB2 and MdSUT2 transgenic plants produced more soluble sugars than controls. Furthermore, MdAREB2 promoted the accumulation of sucrose and soluble sugars in an MdSUT2 -dependent manner. Our results demonstrate that the ABA-responsive transcription factor MdAREB2 directly activates the expression of amylase and sugar transporter genes to promote soluble sugar accumulation, suggesting a mechanism by which ABA regulates sugar accumulation in plants. © 2017 American Society of Plant Biologists. All Rights Reserved.

  4. Profiling and relationship of water-soluble sugar and protein compositions in soybean seeds.

    Science.gov (United States)

    Yu, Xiaomin; Yuan, Fengjie; Fu, Xujun; Zhu, Danhua

    2016-04-01

    Sugar and protein are important quality traits in soybean seeds for making soy-based food products. However, the investigations on both compositions and their relationship have rarely been reported. In this study, a total of 35 soybean germplasms collected from Zhejiang province of China, were evaluated for both water-soluble sugar and protein. The total water-soluble sugar (TWSS) content of the germplasms studied ranged from 84.70 to 140.91 mg/g and the water-soluble protein (WSP) content varied from 26.5% to 36.0%. The WSP content showed positive correlations with the TWSS and sucrose contents but negative correlations with the fructose and glucose contents. The clustering showed the 35 germplasms could be divided into four groups with specific contents of sugar and protein. The combination of water-soluble sugar and protein profiles provides useful information for future breeding and genetic research. This investigation will facilitate future work for seed quality improvement. Copyright © 2015. Published by Elsevier Ltd.

  5. Water deficit modifies the carbon isotopic composition of lipids, soluble sugars and leaves of Copaifera langsdorffii Desf. (Fabaceae

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    Angelo Albano da Silva Bertholdi

    2017-11-01

    Full Text Available ABSTRACT Water deficit is most frequent in forest physiognomies subjected to climate change. As a consequence, several tree species alter tissue water potential, gas exchange and production of carbon compounds to overcome damage caused by water deficiency. The working hypothesis, that a reduction in gas exchange by plants experiencing water deficit will affect the composition of carbon compounds in soluble sugars, lipids and vegetative structures, was tested on Copaifera langsdorffii. Stomatal conductance, leaf water potential, and CO2 assimilation rate declined after a period of water deficit. After rehydration, leaf water potential and leaf gas exchange did not recover completely. Water deficit resulted in 13C enrichment in leaves, soluble sugars and root lipids. Furthermore, the amount of soluble sugars and root lipids decreased after water deficit. In rehydration, the carbon isotopic composition and amount of root lipids returned to levels similar to the control. Under water deficit, 13C-enriched in root lipids assists in the adjustment of cellular membrane turgidity and avoids damage to the process of water absorption by roots. These physiological adjustments permit a better understanding of the responses of Copaifera langsdorffi to water deficit.

  6. Comparison of techniques for determination of soluble sugars used in feed for ruminant nutrition

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    Cândida Camila dos Reis

    2015-02-01

    Full Text Available This study aims to evaluate different techniques for determination of soluble sugars (fraction CA in foods used for ruminant nutrition. Feed analyzed were: sugar-cane, bermuda grass, corn meal and soybean meal. Dry matter (DM, ash, ether extract (EE and crude protein (CP were determined to make possible the calculation of total carbohydrates concentration in the samples. The soluble carbohydrate fraction were determined in 15 repetitions of each sample by two different analytical techniques. One technique based on soluble carbohydrates extraction and its quantification by spectrophotometry after chelation of the sugar with anthrone; and another one that uses phenol as the chelating agent. The experiment were conducted in a completely randomized design and the data submitted to the two-factor factorial analysis of variance (? = 0.05, with the different feeds and the two techniques as factors. There was no statistical difference between techniques, but the effect of the feed and the interaction feed x technique were significant. So, a new analysis of variance was conducted to test the difference between techniques in each feed separately. Only soybean meal did not show statistical difference between the water soluble sugars determined by the two techniques.

  7. Method to produce water-soluble sugars from biomass using solvents containing lactones

    Science.gov (United States)

    Dumesic, James A.; Luterbacher, Jeremy S.

    2017-08-08

    A process to produce an aqueous solution of carbohydrates that contains C6-sugar-containing oligomers, C6 sugar monomers, C5-sugar-containing oligomers, C5 sugar monomers, or any combination thereof is presented. The process includes the steps of reacting biomass or a biomass-derived reactant with a solvent system including a lactone and water, and an acid catalyst. The reaction yields a product mixture containing water-soluble C6-sugar-containing oligomers, C6-sugar monomers, C5-sugar-containing oligomers, C5-sugar monomers, or any combination thereof. A solute is added to the product mixture to cause partitioning of the product mixture into an aqueous layer containing the carbohydrates and a substantially immiscible organic layer containing the lactone.

  8. Effect of water deficit stress on proline contents, soluble sugars ...

    African Journals Online (AJOL)

    Effect of water deficit stress on proline contents, soluble sugars, chlorophyll and grain yield of sunflower ... Journal Home > Vol 11, No 1 (2012) > ... The objective of the present work was to determine the mechanisms of tolerance of four ...

  9. Comparative transcriptome analysis reveals key genes potentially related to soluble sugar and organic acid accumulation in watermelon

    Science.gov (United States)

    Gao, Lei; Zhao, Shengjie; Lu, Xuqiang; He, Nan; Zhu, Hongju; Dou, Junling

    2018-01-01

    Soluble sugars and organic acids are important components of fruit flavor and have a strong impact on the overall organoleptic quality of watermelon (Citrullus lanatus) fruit. Several studies have analyzed the expression levels of the genes related to soluble sugar accumulation and the dynamic changes in their content during watermelon fruit development and ripening. Nevertheless, to date, there have been no reports on the organic acid content in watermelon or the genes regulating their synthesis. In this study, the soluble sugars and organic acids in watermelon were measured and a comparative transcriptome analysis was performed to identify the key genes involved in the accumulation of these substances during fruit development and ripening. The watermelon cultivar ‘203Z’ and its near-isogenic line (NIL) ‘SW’ (in the ‘203Z’ background) were used as experimental materials. The results suggested that soluble sugar consist of fructose, glucose and sucrose while malic-, citric-, and oxalic acids are the primary organic acids in watermelon fruit. Several differentially expressed genes (DEGs) related to soluble sugar- and organic acid accumulation and metabolism were identified. These include the DEGs encoding raffinose synthase, sucrose synthase (SuSy), sucrose-phosphate synthase (SPSs), insoluble acid invertases (IAI), NAD-dependent malate dehydrogenase (NAD-cyt MDH), aluminum-activated malate transporter (ALMT), and citrate synthase (CS). This is the first report addressing comparative transcriptome analysis via NILs materials in watermelon fruit. These findings provide an important basis for understanding the molecular mechanism that leads to soluble sugar and organic acid accumulation and metabolism during watermelon fruit development and ripening. PMID:29324867

  10. Comparative transcriptome analysis reveals key genes potentially related to soluble sugar and organic acid accumulation in watermelon.

    Directory of Open Access Journals (Sweden)

    Lei Gao

    Full Text Available Soluble sugars and organic acids are important components of fruit flavor and have a strong impact on the overall organoleptic quality of watermelon (Citrullus lanatus fruit. Several studies have analyzed the expression levels of the genes related to soluble sugar accumulation and the dynamic changes in their content during watermelon fruit development and ripening. Nevertheless, to date, there have been no reports on the organic acid content in watermelon or the genes regulating their synthesis. In this study, the soluble sugars and organic acids in watermelon were measured and a comparative transcriptome analysis was performed to identify the key genes involved in the accumulation of these substances during fruit development and ripening. The watermelon cultivar '203Z' and its near-isogenic line (NIL 'SW' (in the '203Z' background were used as experimental materials. The results suggested that soluble sugar consist of fructose, glucose and sucrose while malic-, citric-, and oxalic acids are the primary organic acids in watermelon fruit. Several differentially expressed genes (DEGs related to soluble sugar- and organic acid accumulation and metabolism were identified. These include the DEGs encoding raffinose synthase, sucrose synthase (SuSy, sucrose-phosphate synthase (SPSs, insoluble acid invertases (IAI, NAD-dependent malate dehydrogenase (NAD-cyt MDH, aluminum-activated malate transporter (ALMT, and citrate synthase (CS. This is the first report addressing comparative transcriptome analysis via NILs materials in watermelon fruit. These findings provide an important basis for understanding the molecular mechanism that leads to soluble sugar and organic acid accumulation and metabolism during watermelon fruit development and ripening.

  11. Comparative transcriptome analysis reveals key genes potentially related to soluble sugar and organic acid accumulation in watermelon.

    Science.gov (United States)

    Gao, Lei; Zhao, Shengjie; Lu, Xuqiang; He, Nan; Zhu, Hongju; Dou, Junling; Liu, Wenge

    2018-01-01

    Soluble sugars and organic acids are important components of fruit flavor and have a strong impact on the overall organoleptic quality of watermelon (Citrullus lanatus) fruit. Several studies have analyzed the expression levels of the genes related to soluble sugar accumulation and the dynamic changes in their content during watermelon fruit development and ripening. Nevertheless, to date, there have been no reports on the organic acid content in watermelon or the genes regulating their synthesis. In this study, the soluble sugars and organic acids in watermelon were measured and a comparative transcriptome analysis was performed to identify the key genes involved in the accumulation of these substances during fruit development and ripening. The watermelon cultivar '203Z' and its near-isogenic line (NIL) 'SW' (in the '203Z' background) were used as experimental materials. The results suggested that soluble sugar consist of fructose, glucose and sucrose while malic-, citric-, and oxalic acids are the primary organic acids in watermelon fruit. Several differentially expressed genes (DEGs) related to soluble sugar- and organic acid accumulation and metabolism were identified. These include the DEGs encoding raffinose synthase, sucrose synthase (SuSy), sucrose-phosphate synthase (SPSs), insoluble acid invertases (IAI), NAD-dependent malate dehydrogenase (NAD-cyt MDH), aluminum-activated malate transporter (ALMT), and citrate synthase (CS). This is the first report addressing comparative transcriptome analysis via NILs materials in watermelon fruit. These findings provide an important basis for understanding the molecular mechanism that leads to soluble sugar and organic acid accumulation and metabolism during watermelon fruit development and ripening.

  12. Light exposure during storage preserving soluble sugar and l-ascorbic acid content of minimally processed romaine lettuce (Lactuca sativa L.var. longifolia).

    Science.gov (United States)

    Zhan, Lijuan; Hu, Jinqiang; Ai, Zhilu; Pang, Lingyun; Li, Yu; Zhu, Meiyun

    2013-01-01

    Minimally processed romaine lettuce (MPRL) leaves were stored in light condition (2500lux) or darkness at 4°C for 7d. Light exposure significantly delayed the degradation of chlorophyll and decrease of glucose, reducing sugar, and sucrose content, and thus preserved more total soluble solid (TSS) content at the end of storage in comparison with darkness. While, it did not influenced starch content that progressively decreased over time. The l-ascorbic acid (AA) accumulated in light-stored leaves, but deteriorated in dark-stored leaves during storage. The dehydroascorbic acid (DHA) increased in all leaves stored in both light and dark condition, of which light condition resulted in less DHA than darkness. In addition, the fresh weight loss and dry matter significantly increased and these increases were accelerated by light exposure. Conclusively, light exposure in applied intensity effectively alleviated MPRL quality deterioration by delaying the decreases of pigments, soluble sugar, TSS content and accumulating AA. Copyright © 2012 Elsevier Ltd. All rights reserved.

  13. Multiple Roles of Soluble Sugars in the Establishment of Gunnera-Nostoc Endosymbiosis1[OA

    Science.gov (United States)

    Khamar, Hima J.; Breathwaite, Erick K.; Prasse, Christine E.; Fraley, Elizabeth R.; Secor, Craig R.; Chibane, Fairouz L.; Elhai, Jeff; Chiu, Wan-Ling

    2010-01-01

    Gunnera plants have the unique ability to form endosymbioses with N2-fixing cyanobacteria, primarily Nostoc. Cyanobacteria enter Gunnera through transiently active mucilage-secreting glands on stems. We took advantage of the nitrogen (N)-limitation-induced gland development in Gunnera manicata to identify factors that may enable plant tissue to attract and maintain cyanobacteria colonies. Cortical cells in stems of N-stressed Gunnera plants were found to accumulate a copious amount of starch, while starch in the neighboring mature glands was nearly undetectable. Instead, mature glands accumulated millimolar concentrations of glucose (Glc) and fructose (Fru). Successful colonization by Nostoc drastically reduced sugar accumulation in the surrounding tissue. Consistent with the abundance of Glc and Fru in the gland prior to Nostoc colonization, genes encoding key enzymes for sucrose and starch hydrolysis (e.g. cell wall invertase, α-amylase, and starch phosphorylase) were expressed at higher levels in stem segments with glands than those without. In contrast, soluble sugars were barely detectable in mucilage freshly secreted from glands. Different sugars affected Nostoc’s ability to differentiate motile hormogonia in a manner consistent with their locations. Galactose and arabinose, the predominant constituents of polysaccharides in the mucilage, had little or no inhibitory effect on hormogonia differentiation. On the other hand, soluble sugars that accumulated in gland tissue, namely sucrose, Glc, and Fru, inhibited hormogonia differentiation and enhanced vegetative growth. Results from this study suggest that, in an N-limited environment, mature Gunnera stem glands may employ different soluble sugars to attract Nostoc and, once the cyanobacteria are internalized, to maintain them in the N2-fixing vegetative state. PMID:20833727

  14. Consumption of added sugars is decreasing in the United States.

    Science.gov (United States)

    Welsh, Jean A; Sharma, Andrea J; Grellinger, Lisa; Vos, Miriam B

    2011-09-01

    The consumption of added sugars (caloric sweeteners) has been linked to obesity, diabetes, and heart disease. Little is known about recent consumption trends in the United States or how intakes compare with current guidelines. We examined trends in intakes of added sugars in the United States over the past decade. A cross-sectional study of US residents ≥2 y of age (n = 42,316) was conducted by using dietary data from NHANES 1999-2008 (five 2-y cycles) and data for added-sugar contents from the MyPyramid Equivalents Database. Mean intakes of added sugars (grams and percentage of total energy intake) were weighted to obtain national estimates over time across age, sex, and race-ethnic groups. Linear trends were tested by using Wald's F tests. Between 1999-2000 and 2007-2008, the absolute intake of added sugars decreased from a mean (95% CI) of 100.1 g/d (92.8, 107.3 g/d) to 76.7 g/d (71.6, 81.9 g/d); two-thirds of this decrease, from 37.4 g/d (32.6, 42.1 g/d) to 22.8 g/d (18.4, 27.3 g/d), resulted from decreased soda consumption (P-linear trend added sugars to increase over the study period (P-linear trend = 0.003), although the peak consumption reached only 0.15 g/d (0.08, 0.22 g/d). The percentage of total energy from added sugars also decreased from 18.1% (16.9%, 19.3%) to 14.6% (13.7%, 15.5%) (P-linear trend added sugars in the United States decreased between 1999-2000 and 2007-2008, primarily because of a reduction in soda consumption, mean intakes continue to exceed recommended limits.

  15. Solubility data and modeling for sugar alcohols in ionic liquids

    International Nuclear Information System (INIS)

    Okuniewski, Marcin; Ramjugernath, Deresh; Naidoo, Paramespri; Domańska, Urszula

    2014-01-01

    Highlights: • Solubility of D-sorbitol and xylitol in six ILs. • The (liquid + liquid) phase equilibrium of (SA + IL) with UCST. • Interesting properties of [BMIM][TDI] IL. • The correlation with NRTL model. - Abstract: Ionic liquids (ILs) are novel media characterized by strong interactions with different organic substances which leads to a wide spectrum of applications involving extraction. Ionic liquids have been used as a solvent for sugar alcohols, sugars and hydrates. This work demonstrates the experimental and theoretical study of (liquid + liquid) phase equilibria for two sugar alcohols, D-sorbitol and xylitol in a few ILs based on different cations and anions (namely, 1-ethyl-1-methylpiperidinium bis(trifluoromethylsulfonyl)imide [EMPIP][NTf 2 ], 1-hexyl-1-methylpiperidinium bis(trifluoromethylsulfonyl)imide [HMPIP][NTf 2 ], N-hexylquinolinium bis(trifluoromethylsulfonyl)imide [HQuin][NTf 2 ], N-hexylisoquinolinium bis(trifluoromethylsulfonyl)imide [HiQuin][NTf 2 ], 1-butyl-1-methylimidazolium 4,5-dicyano-2-(trifluoromethyl)-imidazolide [BMIM][TDI] and 1-(cyanomethyl)-3-methylimidazolium 4,5-dicyano-2-(trifluoromethyl)-imidazolide [CCNMIM][TDI]). This study was conducted to assess the applicability of the studied ILs for dissolution of these biomass-related materials. (Liquid + liquid) phase equilibrium diagrams (LLE) in binary systems (sugar alcohol + ionic liquid) were measured using the dynamic technique. The influence of the chemical structure of both the ionic liquids and sugar alcohols were established and is discussed

  16. Atmospheric carbon dioxide changes photochemical activity, soluble sugars and volatile levels in broccoli (Brassica oleracea var. italica).

    Science.gov (United States)

    Krumbein, Angelika; Kläring, Hans-Peter; Schonhof, Ilona; Schreiner, Monika

    2010-03-24

    Atmospheric carbon dioxide (CO(2)) concentration is an environmental factor currently undergoing dramatic changes. The objective of the present study was to determine the effect of doubling the ambient CO(2) concentration on plant photochemistry as measured by photochemical quenching coefficient (qP), soluble sugars and volatiles in broccoli. Elevated CO(2) concentration increased qP values in leaves by up to 100% and 89% in heads, while glucose and sucrose in leaves increased by about 60%. Furthermore, in broccoli heads elevated CO(2) concentration induced approximately a 2-fold increase in concentrations of three fatty acid-derived C(7) aldehydes ((E)-2-heptenal, (E,Z)-2,4-heptadienal, (E,E)-2,4-heptadienal), two fatty acid-derived C(5) alcohols (1-penten-3-ol, (Z)-2-pentenol), and two amino acid-derived nitriles (phenyl propanenitrile, 3-methyl butanenitrile). In contrast, concentrations of the sulfur-containing compound 2-ethylthiophene and C(6) alcohol (E)-2-hexenol decreased. Finally, elevated CO(2) concentration increased soluble sugar concentrations due to enhanced photochemical activity in leaves and heads, which may account for the increased synthesis of volatiles.

  17. Consumption of added sugars is decreasing in the United States1234

    Science.gov (United States)

    Sharma, Andrea J; Grellinger, Lisa; Vos, Miriam B

    2011-01-01

    Background: The consumption of added sugars (caloric sweeteners) has been linked to obesity, diabetes, and heart disease. Little is known about recent consumption trends in the United States or how intakes compare with current guidelines. Objective: We examined trends in intakes of added sugars in the United States over the past decade. Design: A cross-sectional study of US residents ≥2 y of age (n = 42,316) was conducted by using dietary data from NHANES 1999–2008 (five 2-y cycles) and data for added-sugar contents from the MyPyramid Equivalents Database. Mean intakes of added sugars (grams and percentage of total energy intake) were weighted to obtain national estimates over time across age, sex, and race-ethnic groups. Linear trends were tested by using Wald's F tests. Results: Between 1999–2000 and 2007–2008, the absolute intake of added sugars decreased from a mean (95% CI) of 100.1 g/d (92.8, 107.3 g/d) to 76.7 g/d (71.6, 81.9 g/d); two-thirds of this decrease, from 37.4 g/d (32.6, 42.1 g/d) to 22.8 g/d (18.4, 27.3 g/d), resulted from decreased soda consumption (P-linear trend added sugars to increase over the study period (P-linear trend = 0.003), although the peak consumption reached only 0.15 g/d (0.08, 0.22 g/d). The percentage of total energy from added sugars also decreased from 18.1% (16.9%, 19.3%) to 14.6% (13.7%, 15.5%) (P-linear trend added sugars in the United States decreased between 1999–2000 and 2007–2008, primarily because of a reduction in soda consumption, mean intakes continue to exceed recommended limits. PMID:21753067

  18. Coregulation of soybean vegetative storage protein gene expression by methyl jasmonate and soluble sugars.

    Science.gov (United States)

    Mason, H S; Dewald, D B; Creelman, R A; Mullet, J E

    1992-03-01

    The soybean vegetative storage protein genes vspA and vspB are highly expressed in developing leaves, stems, flowers, and pods as compared with roots, seeds, and mature leaves and stems. In this paper, we report that physiological levels of methyl jasmonate (MeJA) and soluble sugars synergistically stimulate accumulation of vsp mRNAs. Treatment of excised mature soybean (Glycine max Merr. cv Williams) leaves with 0.2 molar sucrose and 10 micromolar MeJA caused a large accumulation of vsp mRNAs, whereas little accumulation occurred when these compounds were supplied separately. In soybean cell suspension cultures, the synergistic effect of sucrose and MeJA on the accumulation of vspB mRNA was maximal at 58 millimolar sucrose and was observed with fructose or glucose substituted for sucrose. In dark-grown soybean seedlings, the highest levels of vsp mRNAs occurred in the hypocotyl hook, which also contained high levels of MeJA and soluble sugars. Lower levels of vsp mRNAs, MeJA, and soluble sugars were found in the cotyledons, roots, and nongrowing regions of the stem. Wounding of mature soybean leaves induced a large accumulation of vsp mRNAs when wounded plants were incubated in the light. Wounded plants kept in the dark or illuminated plants sprayed with dichlorophenyldimethylurea, an inhibitor of photosynthetic electron transport, showed a greatly reduced accumulation of vsp mRNAs. The time courses for the accumulation of vsp mRNAs induced by wounding or sucrose/MeJA treatment were similar. These results strongly suggest that vsp expression is coregulated by endogenous levels of MeJA (or jasmonic acid) and soluble carbohydrate during normal vegetative development and in wounded leaves.

  19. Serum Soluble Corin is Decreased in Stroke.

    Science.gov (United States)

    Peng, Hao; Zhu, Fangfang; Shi, Jijun; Han, Xiujie; Zhou, Dan; Liu, Yan; Zhi, Zhongwen; Zhang, Fuding; Shen, Yun; Ma, Juanjuan; Song, Yulin; Hu, Weidong

    2015-07-01

    Soluble corin was decreased in coronary heart disease. Given the connections between cardiac dysfunction and stroke, circulating corin might be a candidate marker of stroke risk. However, the association between circulating corin and stroke has not yet been studied in humans. Here, we aimed to examine the association in patients wtith stroke and community-based healthy controls. Four hundred eighty-one patients with ischemic stroke, 116 patients with hemorrhagic stroke, and 2498 healthy controls were studied. Serum soluble corin and some conventional risk factors of stroke were examined. Because circulating corin was reported to be varied between men and women, the association between serum soluble corin and stroke was evaluated in men and women, respectively. Patients with ischemic and hemorrhagic stroke had a significantly lower level of serum soluble corin than healthy controls in men and women (all P values, stroke than men in the highest quartile. Women in the lowest quartile of serum soluble corin were also more likely to have ischemic (OR, 3.10; 95% confidence interval, 1.76-5.44) and hemorrhagic (OR, 8.54; 95% confidence interval, 2.35-31.02) stroke than women in the highest quartile. ORs of ischemic and hemorrhagic stroke were significantly increased with the decreasing levels of serum soluble corin in men and women (all P values for trend, stroke compared with healthy controls. Our findings raise the possibility that serum soluble corin may have a pathogenic role in stroke. © 2015 American Heart Association, Inc.

  20. Soluble Sugars as the Carbohydrate Reserve for CAM in Pineapple Leaves 1

    Science.gov (United States)

    Carnal, Nancy Wieland; Black, Clanton C.

    1989-01-01

    Neutral ethanol-soluble sugar pools serve as carbohydrate reserves for Crassulacean acid metabolism (CAM) in pineapple (Ananas comosus (L.) Merr.) leaves. Levels of neutral soluble sugars and glucans fluctuated reciprocally with concentrations of malic acid. Hexose loss from neutral soluble-sugar pools was sufficient to account for malic acid accumulation with about 95% of the required hexose accounted for by turnover of fructose and glucose pools. Hexose loss from starch or starch plus lower molecular weight glucan pools was insufficient to account for nocturnal accumulation of malic acid. The apparent maximum catalytic capacity of pyrophosphate:6-phosphofructokinase (PPi-PFK) at 15°C was about 16 times higher than the mean maximum rate of glycolysis that occurred to support malic acid accumulation in pineapple leaves at night and 12 times higher than the mean maximum rate of hexose turnover from all carbohydrate pools. The apparent maximum catalytic capacity of ATP-PFK at 15°C was about 70% of the activity required to account for the mean maximal rate of hexose turnover from all carbohydrate pools if turnover were completely via glycolysis, and marginally sufficient to account for mean maximal rates of acidification. Therefore, at low night temperatures conducive to CAM and under subsaturating substrate concentrations, PPi-PFK activity, but not ATP-PFK activity, would be sufficient to support the rate of glycolytic carbohydrate processing required for acid accumulation. These data for pineapple establish that there are at least two types of CAM plants with respect to the nature of the carbohydrate reserve utilized to support nighttime CO2 accumulation. The data further indicate that the glycolytic carbohydrate processing that supports acidification proceeds in different subcellular compartments in plants utilizing different carbohydrate reserves. PMID:16666775

  1. Seasonal patterns of reserve and soluble carbohydrates in mature sugar maple (Acer saccharum)

    Science.gov (United States)

    B.L. Wong; K.L. Baggett; A.H. Rye

    2003-01-01

    Sugar maple (Acer saccharum Marsh.) trees exhibit seasonal patterns of production, accumulation, and utilization of nonstructural carbohydrates that are closely correlated with phenological events and (or) physiological processes. The simultaneous seasonal patterns of both reserve and soluble carbohydrates in the leaves, twigs, branches, and trunks of healthy mature...

  2. Countercurrent extraction of soluble sugars from almond hulls and assessment of the bioenergy potential.

    Science.gov (United States)

    Holtman, Kevin M; Offeman, Richard D; Franqui-Villanueva, Diana; Bayati, Andre K; Orts, William J

    2015-03-11

    Almond hulls contain considerable proportions (37% by dry weight) of water-soluble, fermentable sugars (sucrose, glucose, and fructose), which can be extracted for industrial purposes. The maximum optimal solids loading was determined to be 20% for sugar extraction, and the addition of 0.5% (w/v) pectinase aided in maintaining a sufficient free water volume for sugar recovery. A laboratory countercurrent extraction experiment utilizing a 1 h steep followed by three extraction (wash) stages produced a high-concentration (131 g/L fermentable sugar) syrup. Overall, sugar recovery efficiency was 88%. The inner stage washing efficiencies were compatible with solution equilibrium calculations, indicating that efficiency was high. The concentrated sugar syrup was fermented to ethanol at high efficiency (86% conversion), and ethanol concentrations in the broth were 7.4% (v/v). Thin stillage contained 233 g SCOD/L, which was converted to biomethane at an efficiency of 90% with a biomethane potential of 297 mL/g SCODdestroyed. Overall, results suggested that a minima of 49 gal (185 L) ethanol and 75 m(3) methane/t hulls (dry whole hull basis) are achievable.

  3. Coregulation of Soybean Vegetative Storage Protein Gene Expression by Methyl Jasmonate and Soluble Sugars 1

    Science.gov (United States)

    Mason, Hugh S.; DeWald, Daryll B.; Creelman, Robert A.; Mullet, John E.

    1992-01-01

    The soybean vegetative storage protein genes vspA and vspB are highly expressed in developing leaves, stems, flowers, and pods as compared with roots, seeds, and mature leaves and stems. In this paper, we report that physiological levels of methyl jasmonate (MeJA) and soluble sugars synergistically stimulate accumulation of vsp mRNAs. Treatment of excised mature soybean (Glycine max Merr. cv Williams) leaves with 0.2 molar sucrose and 10 micromolar MeJA caused a large accumulation of vsp mRNAs, whereas little accumulation occurred when these compounds were supplied separately. In soybean cell suspension cultures, the synergistic effect of sucrose and MeJA on the accumulation of vspB mRNA was maximal at 58 millimolar sucrose and was observed with fructose or glucose substituted for sucrose. In dark-grown soybean seedlings, the highest levels of vsp mRNAs occurred in the hypocotyl hook, which also contained high levels of MeJA and soluble sugars. Lower levels of vsp mRNAs, MeJA, and soluble sugars were found in the cotyledons, roots, and nongrowing regions of the stem. Wounding of mature soybean leaves induced a large accumulation of vsp mRNAs when wounded plants were incubated in the light. Wounded plants kept in the dark or illuminated plants sprayed with dichlorophenyldimethylurea, an inhibitor of photosynthetic electron transport, showed a greatly reduced accumulation of vsp mRNAs. The time courses for the accumulation of vsp mRNAs induced by wounding or sucrose/MeJA treatment were similar. These results strongly suggest that vsp expression is coregulated by endogenous levels of MeJA (or jasmonic acid) and soluble carbohydrate during normal vegetative development and in wounded leaves. ImagesFigure 1Figure 4Figure 5 PMID:16668757

  4. The relationship between growth and soluble sugar concentration of Aloe vera plants grown under three levels of irradiance

    Energy Technology Data Exchange (ETDEWEB)

    Paez, A.; Gebre, G.M.; Tschaplinski, T.J. (Universidad del Zulia (Venezuela))

    1994-06-01

    The CAM plant Aloe vera was vegetatively propagated and grown under three irradiances: full sun, partial and deep shade (30% and 10% of ambient light, respectively) to determine the effect on growth, biomass allocation, and sugar concentration. After one year, the plants were harvested to determine final dry weight and the sugar concentration of the leaf mucilaginous gel. Plants grown under full sun produced twice the total dry weight of those grown under partial shade, with the difference equally partitioned between the shoot and root. Plants grown under full sun also produced thicker leaves, and more numerous and large auxiliary shoots. The dry weight of plants grown under deep shade was 8.6% that of plants grown under full sun, which was directly proportional to the irradiance received. Partial shade increased the number and length of leaves produced on the primary shoot, but the allocation of carbon to roots was the lowest of all treatments. Partial shade reduced the total sugar concentration of the leaf gel matrix to 34% that of plants under full sun, due to reductions in all sugars measured. Glucose was the most abundant soluble sugar, with its relative contribution to the total pool increasing under shade. In summary, the proportional effects of partial shading were greater on soluble sugar concentrations than on the total plant biomass produced.

  5. Effect of drought stress on leaf soluble sugar content, leaf rolling index and relative water content of proso millet (Panicum miliaceum L. genotypes

    Directory of Open Access Journals (Sweden)

    mohamad javad seghatol eslami

    2009-06-01

    Full Text Available With respect to water shortage in arid and semi- arid regions, the study about drought stress effects on crop plants and selection of resistance cultivars, are among the most important goals in the agricultural researches. In order to examine drought stress effects on millet, an experiment was conducted in Birjand and Sarbisheh, simultaneously. In this experiment, five irrigation treatments (well-watered, drought stress in vegetative stage, in ear emergence stage, in seed filling stage and in vegetative and seed filling stage and five proso millet genotypes (Native, K-C-M.2, K-C-M.4, K-C-M.6 and K-C-M.9 were compared in a split plot design along with three replications. Drought stress increased grain protein content, leaf rolling index and soluble sugars concentration and decreased seed germination and leaf RWC. Although seed protein content and germination percentage of genotypes were not significantly different, there were some differences among leaf rolling index, RWC and soluble sugar content of these genotypes. The results of this study indicated that leaf sugar content, RWC and leaf rolling index can not be considered as the only parameters for selection of high yield genotypes. Therefore, it is recommended that some other factors should also be used apart from the above mentioned ones.

  6. Predicting the solubility of mixtures of sugars and their replacers using the Flory-Huggins theory

    NARCIS (Netherlands)

    Sman, van der R.G.M.

    2017-01-01

    In this paper we investigate whether the Flory-Huggins theory can describe the thermodynamics of solutions of simple carbohydrates, like sugars and polyols. In particular, we focus on the description of the solubility of the carbohydrates in water. This is investigated for both binary and ternary

  7. Chemical constituents: water-soluble vitamins, free amino acids and sugar profile from Ganoderma adspersum.

    Science.gov (United States)

    Kıvrak, İbrahim

    2015-01-01

    Ganoderma adspersum presents a rigid fruiting body owing to chitin content and having a small quantity of water or moisture. The utility of bioactive constituent of the mushroom can only be available by extraction for human usage. In this study, carbohydrate, water-soluble vitamin compositions and amino acid contents were determined in G. adspersum mushroom. The composition in individual sugars was determined by HPLC-RID, mannitol (13.04 g/100 g) and trehalose (10.27 g/100 g) being the most abundant sugars. The examination of water-soluble vitamins and free amino acid composition was determined by UPLC-ESI-MS/MS. Essential amino acid constituted 67.79% of total amino acid, which is well worth the attention with regard to researchers and consumers. In addition, G. adspersum, which is also significantly rich in B group vitamins and vitamin C, can provide a wide range of notable applications in the pharmaceutics, cosmetics, food and dietary supplement industries. G. adspersum revealed its value for pharmacy and nutrition fields.

  8. Enzyme Enzyme activities in relation to sugar accumulation in tomato

    International Nuclear Information System (INIS)

    Alam, M.J.; Rahman, M.H.; Mamun, M.A.; Islam, K.

    2006-01-01

    Enzyme activities in tomato juice of five different varieties viz. Ratan, Marglove, BARI-1, BARI-5 and BARI-6, in relation to sugar accumulation were investigated at different maturity stages. The highest amount of invertase and beta-galactosidase was found in Marglove and the lowest in BARI- 6 at all maturity stages. Total soluble sugar and sucrose contents were highest in BARI-1 and lowest in BARI-6. The activity of amylase was maximum in Ratan and minimum in Marglove. Protease activity was highest in Ratan and lowest in BARI-6. BARI-1 contained the highest cellulase activity and the lowest in BARI-5. The amount of total soluble sugar and sucrose increased moderately from premature to ripe stage. The activities of amylase and cellulase increased up to the mature stage and then decreased drastically in the ripe stage. The activities of invertase and protease increased sharply from the premature to the ripe stage while the beta-galactosidase activity decreased remarkably. No detectable amount of reducing sugar was present in the premature stage in all cultivars of tomato but increased thereafter upto the ripe stage. The highest reducing sugar was present in BARI-5 in all of the maturity stages. (author)

  9. Effects of bagging on sugar metabolism and the activity of sugar ...

    African Journals Online (AJOL)

    To investigate the effects of bagging on sugar metabolism and the activity of sugar metabolism related enzymes in Qingzhong loquat fruit development, the contents of sucrose, glucose and soluble solids as well as the activities of sugar metabolism related enzymes were evaluated. The content of sucrose, glucose and ...

  10. Impact of the nutrients N and K and soluble sugars on Diabrotica speciosa (Germar) (Coleoptera, Chrysomelidae) and Agrotis ipsilon (Hufnagel) (Lepidoptera, Noctuidae) populations in potato crops, Solanum tuberosum L. (Solanaceae)

    International Nuclear Information System (INIS)

    Azeredo, Edson Henrique de; Lima, Eduardo; Cassino, Paulo Cesar Rodrigues

    2004-01-01

    Impact of the nutrients N and K and soluble sugars on Diabrotica speciosa (Germar) (Coleoptera, Chrysomelidae) and Agrotis ipsilon (Huefnagel) (Lepidoptera, Noctuidae) populations in potato crops, Solanum tuberosum L. (Solanaceae). The occurrence of Diabrotica speciosa (Germar, 1824) and Agrotis ipsilon (Huefnagel, 1767) on the potato cultivars Achat and Monalisa, influenced by nitrogen and potassium dosage, and minimum quantity of soluble sugars, was studied. The following parameters were evaluated: concentration of mineral nutrient and sugar in green leaf, senescent leaf, leaf in abscission, stem, tubercle and total plant using extracts of infusion in ethanol 80%. The largest infestation of D. speciosa larvae was on Monalisa cultivar at 150 kg.ha -1 of N + K with 27.03% at P -1 , in the absence of potassium. On the other hand, high dosage of K reduced the damages by A. ipsilon on Monalisa cultivar. However, it did not influence the storage of soluble sugar. The results indicated that in Achat cultivar the accumulated soluble sugar was reduced, probably sensitized by elevation of potassic fertilization dosing, differing from Monalisa cultivar, in which the influence was by nitrogen dosing. (author)

  11. Co-extraction of soluble and insoluble sugars from energy sorghum based on a hydrothermal hydrolysis process.

    Science.gov (United States)

    Yu, Qiang; Tan, Xuesong; Zhuang, Xinshu; Wang, Qiong; Wang, Wen; Qi, Wei; Zhou, Guixiong; Luo, Yu; Yuan, Zhenhong

    2016-12-01

    A process for co-extraction of soluble and insoluble sugars from energy sorghum (ES) was developed based on hydrothermal hydrolysis (HH). Two series of ES were investigated: one (N) with a high biomass yield displayed a higher recalcitrance to sugar release, whereas the second (T) series was characterized by high sugar extraction. The highest total xylose recoveries of 87.2% and 98.7% were obtained for N-11 and T-106 under hydrolysis conditions of 180°C for 50min and 180°C for 30min, respectively. Moreover, the T series displayed higher enzymatic digestibility (ED) than the N series. The high degree of branching (arabinose/xylose ratio) and acetyl groups in the hemicellulose chains of T-106 would be expected to accelerate sugar release during the HH process. In addition, negative correlations between ED and the lignin content, crystallinity index (CrI) and syringyl/guaiacyl (S/G) lignin ratio were observed. Furthermore, finding ways to overcome the thickness of the cell wall and heterogeneity of its chemical composition distribution would make cellulose more accessible to the enzyme. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Glucose, fructose and sucrose increase the solubility of protein-tannin complexes and at high concentration, glucose and sucrose interfere with bisulphite bleaching of wine pigments.

    Science.gov (United States)

    Harbertson, James F; Yuan, Chunlong; Mireles, Maria S; Hanlin, Rachel L; Downey, Mark O

    2013-05-01

    Wines were modified with increasing sugar concentrations and decreasing tannin concentrations and analysed by a combination of protein precipitation and bisulphite bleaching. Increasing sugar concentration decreased the precipitation of tannin and protein-precipitable polymeric pigments (PPP). The use of a hydrogen bond disruptor (urea) to reduce protein-tannin and protein-pigment complex formation showed that the effect of sugar concentration occurred by increasing the solubility of the tannin-protein complex, not by interfering with protein-tannin complex formation. By increasing the solubility of pigment-protein complexes, non-protein-precipitable polymeric pigments (nPPP) appeared to increase. There was also an increase in total polymeric pigments at each tannin concentration with increasing glucose and sucrose concentration, indicating that sugar concentration might also affect bisulphite bleaching of wine pigments. While a significant effect of sugar concentration on tannin-protein complex solubility was observed, these effects were greatest at sugar concentrations far in excess of normal wine making conditions. Under normal wine making conditions, sugar concentration will have a negligible effect on protein-precipitable tannin, PPP and nPPP concentrations. Copyright © 2012 Elsevier Ltd. All rights reserved.

  13. Relationships between soluble sugar concentrations in roots and ecosystem stress for first-year sugar maple seedlings

    Energy Technology Data Exchange (ETDEWEB)

    McLaughlin, J.W.; Reed, D.D.; Jurgensen, M.F.; Mroz, G.D.; Bagley, S.T. [Michigan Technological University, Houghton, MI (United States). School of Forestry and Wood Products

    1996-03-01

    Accumulation of reducing sugars (i.e. glucose and fructose) in plant roots has been consistently correlated with forest dieback and decline and, therefore, has potential as a biological indicator of ecosystem stress. In this study, the relationships between acidic deposition and `natural` (temperature, mycorrhizae, and nutrition) factors with first-year sugar maple seedling root sugar concentrations and growth were assessed in two sugar maple dominated forests in Michigan. Seedlings at the southern site (Wellston) had greater root growth, phosphorus, total sugar, and sucrose concentrations in roots, but lower reducing sugar concentration in roots. In addition, percent root length colonized by vesicular-arbuscular mycorrhizal fungi was less than that found for seedlings growing at the northern site (Alberta). Throughfall deposition of nitrate, sulfate, and hydrogen ions was not significantly correlated with seedling total or reducing sugar concentration. Total sugar concentration in seedling roots was positively correlated with air and soil temperatures at the southern site, but not at the northern site. Seedling tissue phosphorus concentration was correlated with total sugars at both sites, with sucrose at the southern site, and reducing sugars at the northern site. Mycorrhizal colonization rates at the Alberta site were positively correlated with reducing sugar concentration in seedling roots and negatively correlated with sucrose concentration. The results suggest that differences in seedling root sugar concentrations in these two forests are related to seedling root growth and are most likely due to ecological variables, such as available soil phosphorus, temperature, and growing season length through some complex interaction with mycorrhizae rather than acidic deposition stress. 56 refs., 3 figs.

  14. Ageing mechanisms in chickpea seeds: Relationship of sugar hydrolysis and lipid peroxidation with Amadori and Millard reactions

    Directory of Open Access Journals (Sweden)

    mahdi shaaban

    2017-05-01

    Full Text Available This experiment was performed in order to study on ageing mechanisms of chickpea seeds (Cicer arietinum L. in natural storage and accelerated ageing conditions in seed laboratory of Gorgan Agricultural Science and Natural Resources, Gorgan, Iran at 2015. Experiment was in completely randomized design arrangement with four replications. Treatments were 2 and 4 years natural storage and 1-5 days of accelerated ageing with control treatment. The results showed that with increasing of natural storage and accelerated ageing duration, germination percentage was decreased. Increasing of ageing duration decreased soluble sugars, non-reducing sugars and soluble proteins but lipid peroxidation, reducing sugars, protein carbonylation and Amadori and Millard reaction were increased. In natural storage condition lipid peroxidation was more than sugar hydrolysis but in accelerated ageing condition sugar hydrolysis was more than lipid peroxidation. These results show that the main reason of Amadori and Millard reaction in chickpea seeds in natural storage condition is lipid peroxidation and in accelerated ageing condition is sugar hydrolysis. Also, the results showed that Amadori reaction in natural storage condition was more than Amadori reaction and in accelerated ageing condition Millard reaction was more than Amadori reaction. The results of the present study showed that sever Millard reaction after Amadori reaction induced higher damage on seed and results to more decrease of seed viability and reduce of seed germination percentage in accelerated ageing than natural storage.

  15. Prediction of Starch, Soluble Sugars and Amino Acids in Potatoes (Solanum tuberosum L.) Using Hyperspectral Imaging, Dielectric and LF-NMR Methodologies

    DEFF Research Database (Denmark)

    Kjær, Anders; Nielsen, Glenn; Stærke, Søren

    2016-01-01

    Handling and processing of potatoes is performed in increasingly large and more automated facilities, and the industry calls for more automated machinery for quality assessment and sorting by concentration of starch, soluble sugars, protein, amino acids etc. of the potato tubers. The present study...... cultivars were simultaneously sampled for analyses of content and scanned by the five different scanning methods. The resulting multivariate dataset was used to estimate the prediction ability of the individual scanning methods on starch-related parameters, selected simple sugars, selected amino acids......, conductivity of pressed cell sap and cell sizes. Results showed that most types of spectral analyses had relatively high potential for predicting the starch-related parameters and medium potential for predicting the concentration of the reducing sugars fructose and glucose. Most methods showed medium potential...

  16. Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass.

    Science.gov (United States)

    Kim, Youngmi; Ximenes, Eduardo; Mosier, Nathan S; Ladisch, Michael R

    2011-04-07

    Liquid hot water, steam explosion, and dilute acid pretreatments of lignocellulose generate soluble inhibitors which hamper enzymatic hydrolysis as well as fermentation of sugars to ethanol. Toxic and inhibitory compounds will vary with pretreatment and include soluble sugars, furan derivatives (hydroxymethyl fulfural, furfural), organic acids (acetic, formic and, levulinic acid), and phenolic compounds. Their effect is seen when an increase in the concentration of pretreated biomass in a hydrolysis slurry results in decreased cellulose conversion, even though the ratio of enzyme to cellulose is kept constant. We used lignin-free cellulose, Solka Floc, combined with mixtures of soluble components released during pretreatment of wood, to prove that the decrease in the rate and extent of cellulose hydrolysis is due to a combination of enzyme inhibition and deactivation. The causative agents were extracted from wood pretreatment liquid using PEG surfactant, activated charcoal or ethyl acetate and then desorbed, recovered, and added back to a mixture of enzyme and cellulose. At enzyme loadings of either 1 or 25mg protein/g glucan, the most inhibitory components, later identified as phenolics, decreased the rate and extent of cellulose hydrolysis by half due to both inhibition and precipitation of the enzymes. Full enzyme activity occurred when the phenols were removed. Hence detoxification of pretreated woods through phenol removal is expected to reduce enzyme loadings, and therefore reduce enzyme costs, for a given level of cellulose conversion. Copyright © 2011 Elsevier Inc. All rights reserved.

  17. Evaluation of proline, chlorophyll, soluble sugar content and uptake of nutrients in the German chamomile (Matricaria chamomilla L. under drought stress and organic fertilizer treatments

    Directory of Open Access Journals (Sweden)

    Amin Salehi

    2016-10-01

    Conclusions: Totally, organic fertilization by vermicompost could partly alleviate the effect of drought stress on chamomile by increasing N, P and K uptake and leaf soluble sugar, especially in stressed treatments.

  18. Cold-season patterns of reserve and soluble carbohydrates in sugar maple and ice-damaged trees of two age classes following drought

    Science.gov (United States)

    B. L. Wong; K. L. Baggett; A. H. Rye

    2009-01-01

    This study examines the effects of summer drought on the composition and profiles of cold-season reserve and soluble carbohydrates in sugar maple (Acer saccharum Marsh.) trees (50-100 years old or ~200 years old) in which the crowns were nondamaged or damaged by the 1998 ice storm. The overall cold season reserve...

  19. Lipid and sugar profiles of various barley cultivars (Hordeum vulgare

    Directory of Open Access Journals (Sweden)

    Pastor Kristian A.

    2015-01-01

    Full Text Available The lipid components and soluble sugars in flour samples of different cultivars of barley (Hordeum vulgare, involving winter malting barley, winter forage barley, spring barley, and hulless barley, were identified. Fatty acids were extracted from flour samples with n-hexane, and derivatized into volatile methyl esters, using TMSH (trimethylsulfonium hydroxide in methanol. Soluble sugars were extracted from defatted and dried samples of barley flour with 96% ethanol, and further derivatized into the corresponding trimethylsilyl (TMS oximes, using hydroxylamine hydrochloride solution and BSTFA (N,O-bis-(trimethylsilyl-trifluoroacetamide. The hexane and alcoholic extracts of barley cultivars were analyzed by GC-MS system. Lipid and sugar compositions were very similar in all barley cultivars. Therefore, multivariate analysis was applied to numerical values of automatically integrated areas of the identified fatty acid methyl esters and TMS oximes of soluble sugars. The application of hierarchical cluster analysis showed a great similarity between the investigated flour samples of barley cultivars, according to their fatty acid content (0.96. Also, significant, but somewhat less similarity was observed regarding the content of soluble sugars (0.70. These preliminary results indicate the possibility of distinguishing flour made of barley, regardless of the variety, from flours made of other cereal species, just by the analysis of the contents of fatty acids and soluble sugars.[Projekat Ministarstva nauke Republike Srbije, br. TR 31066

  20. Soluble Sugars as the Carbohydrate Reserve for CAM in Pineapple Leaves : Implications for the Role of Pyrophosphate:6-Phosphofructokinase in Glycolysis.

    Science.gov (United States)

    Carnal, N W; Black, C C

    1989-05-01

    Neutral ethanol-soluble sugar pools serve as carbohydrate reserves for Crassulacean acid metabolism (CAM) in pineapple (Ananas comosus (L.) Merr.) leaves. Levels of neutral soluble sugars and glucans fluctuated reciprocally with concentrations of malic acid. Hexose loss from neutral soluble-sugar pools was sufficient to account for malic acid accumulation with about 95% of the required hexose accounted for by turnover of fructose and glucose pools. Hexose loss from starch or starch plus lower molecular weight glucan pools was insufficient to account for nocturnal accumulation of malic acid. The apparent maximum catalytic capacity of pyrophosphate:6-phosphofructokinase (PPi-PFK) at 15 degrees C was about 16 times higher than the mean maximum rate of glycolysis that occurred to support malic acid accumulation in pineapple leaves at night and 12 times higher than the mean maximum rate of hexose turnover from all carbohydrate pools. The apparent maximum catalytic capacity of ATP-PFK at 15 degrees C was about 70% of the activity required to account for the mean maximal rate of hexose turnover from all carbohydrate pools if turnover were completely via glycolysis, and marginally sufficient to account for mean maximal rates of acidification. Therefore, at low night temperatures conducive to CAM and under subsaturating substrate concentrations, PPi-PFK activity, but not ATP-PFK activity, would be sufficient to support the rate of glycolytic carbohydrate processing required for acid accumulation. These data for pineapple establish that there are at least two types of CAM plants with respect to the nature of the carbohydrate reserve utilized to support nighttime CO(2) accumulation. The data further indicate that the glycolytic carbohydrate processing that supports acidification proceeds in different subcellular compartments in plants utilizing different carbohydrate reserves.

  1. Dynamics of sugar-metabolic enzymes and sugars accumulation during watermelon (citrullus lanatus) fruit development

    International Nuclear Information System (INIS)

    Zhang, H.

    2016-01-01

    We analyzed sugar accumulation and the activities of sugar-metabolic enzymes in ripening fruits of three cultivars of watermelon; a high-sugar type w2, a low-sugar type (w1), and their hybrid. In w2, the glucose and fructose contents were higher than the sucrose content in the earlier stage of fruit development, and fruit growth was accompanied by increases in glucose, fructose, and sucrose contents. The sucrose content increased substantially after 20 days after anthesis (DAA) and it was the main soluble sugar in mature fruit (sucrose: hexoses ratio, 0.71). In W, the fructose and glucose contents were significantly higher than the sucrose content in mature fruit (sucrose: hexoses ratio, 0.25). Comparing the two parent cultivars, sucrose was the most important factor affecting the total sugar content in mature fruit, although glucose and fructose also contributed to total sugar contents. The fructose and glucose contents in the fruit of F1 were mid-way between those of their parents, while the sucrose content was closer to that of W (sucrose:hexoses ratio in F1, 0.26). In the early stage of fruit development of W2, the activities of acid invertase and neutral invertase were higher than those of sucrose synthase and sucrose phosphate synthase. After 20 DAA, the acid invertase and neutral invertase activities decreased and those of sucrose synthase and sucrose phosphate synthase increased, leading to increased sucrose content. In W1, the activities of acid invertase and neutral invertase were higher than those of sucrose synthase and sucrose phosphate synthase at the early stage. The sucrose synthase and sucrose phosphate synthase activities were lower in W1 than in W2 at the later stages of fruit development. The patterns of sugar accumulation and sugar-metabolic enzyme activities during fruit development in F1 were similar to those in W1. (author)

  2. Leaching behavior of water-soluble carbohydrates from almond hulls

    Science.gov (United States)

    Over 58% of the dry matter content of the hulls from the commercial almond (Prunus dulcis (Miller) D.A. Webb) is soluble in warm water (50-70°C) extraction. The water-soluble extractables include useful amounts of fermentable sugars (glucose, fructose, sucrose), sugar alcohols (inositol and sorbito...

  3. Water-Deficit Tolerance in Sweet Potato [Ipomoea batatas (L. Lam.] by Foliar Application of Paclobutrazol: Role of Soluble Sugar and Free Proline

    Directory of Open Access Journals (Sweden)

    Suravoot Yooyongwech

    2017-08-01

    Full Text Available The objective of this study was to elevate water deficit tolerance by improving soluble sugar and free proline accumulation, photosynthetic pigment stabilization, photosynthetic abilities, growth performance and storage root yield in sweet potato cv. ‘Tainung 57’ using a foliar application of paclobutrazol (PBZ. The experiment followed a Completely Randomized Block Design with four concentrations of PBZ: 0 (control, 17, 34, and 51 μM before exposure to 47.5% (well irrigation, 32.3% (mild water deficit or 17.5% (severe water deficit soil water content. A sweet potato cultivar, ‘Japanese Yellow’, with water deficit tolerance attributes was the positive check in this study. Total soluble sugar content (sucrose, glucose, and fructose increased by 3.96-folds in ‘Tainung 57’ plants treated with 34 μM PBZ grown under 32.3% soil water content (SWC compared to the untreated plants, adjusting osmotic potential in the leaves and controlling stomatal closure (represented by stomatal conductance and transpiration rate. In addition, under the same treatment, free proline content (2.15 μmol g-1 FW increased by 3.84-folds when exposed to 17.5% SWC. PBZ had an improved effect on leaf size, vine length, photosynthetic pigment stability, chlorophyll fluorescence, and net photosynthetic rate; hence, delaying wilting symptoms and maintaining storage root yield (26.93 g plant-1 at the harvesting stage. A positive relationship between photon yield of PSII (ΦPSII and net photosynthetic rate was demonstrated (r2 = 0.73. The study concludes that soluble sugar and free proline enrichment in PBZ-pretreated plants may play a critical role as major osmoprotectant to control leaf osmotic potential and stomatal closure when plants were subjected to low soil water content, therefore, maintaining the physiological and morphological characters as well as storage root yield.

  4. Water-Deficit Tolerance in Sweet Potato [Ipomoea batatas (L.) Lam.] by Foliar Application of Paclobutrazol: Role of Soluble Sugar and Free Proline.

    Science.gov (United States)

    Yooyongwech, Suravoot; Samphumphuang, Thapanee; Tisarum, Rujira; Theerawitaya, Cattarin; Cha-Um, Suriyan

    2017-01-01

    The objective of this study was to elevate water deficit tolerance by improving soluble sugar and free proline accumulation, photosynthetic pigment stabilization, photosynthetic abilities, growth performance and storage root yield in sweet potato cv. 'Tainung 57' using a foliar application of paclobutrazol (PBZ). The experiment followed a Completely Randomized Block Design with four concentrations of PBZ: 0 (control), 17, 34, and 51 μM before exposure to 47.5% (well irrigation), 32.3% (mild water deficit) or 17.5% (severe water deficit) soil water content. A sweet potato cultivar, 'Japanese Yellow', with water deficit tolerance attributes was the positive check in this study. Total soluble sugar content (sucrose, glucose, and fructose) increased by 3.96-folds in 'Tainung 57' plants treated with 34 μM PBZ grown under 32.3% soil water content (SWC) compared to the untreated plants, adjusting osmotic potential in the leaves and controlling stomatal closure (represented by stomatal conductance and transpiration rate). In addition, under the same treatment, free proline content (2.15 μmol g -1 FW) increased by 3.84-folds when exposed to 17.5% SWC. PBZ had an improved effect on leaf size, vine length, photosynthetic pigment stability, chlorophyll fluorescence, and net photosynthetic rate; hence, delaying wilting symptoms and maintaining storage root yield (26.93 g plant -1 ) at the harvesting stage. A positive relationship between photon yield of PSII (Φ PSII ) and net photosynthetic rate was demonstrated ( r 2 = 0.73). The study concludes that soluble sugar and free proline enrichment in PBZ-pretreated plants may play a critical role as major osmoprotectant to control leaf osmotic potential and stomatal closure when plants were subjected to low soil water content, therefore, maintaining the physiological and morphological characters as well as storage root yield.

  5. The Changes in Color, Soluble Sugars, Organic Acids, Anthocyanins and Aroma Components in “Starkrimson” during the Ripening Period in China

    Directory of Open Access Journals (Sweden)

    Yulian Liu

    2016-06-01

    Full Text Available “Starkrimson” is a traditional apple cultivar that was developed a long time ago and was widely cultivated in the arid region of the northern Wei River of China. However, little information regarding the quality characteristics of “Starkrimson” fruit has been reported in this area. To elucidate these characteristics, the color, soluble sugars, organic acids, anthocyanins and aroma components were measured during the ripening period through the use of high performance liquid chromatography (HPLC and gas chromatography-mass spectrometry (GC-MS. The results indicated that the changes in anthocyanin contents took place later than the changes in the Commission International Eclairage (CIE parameters. Meanwhile, cyanidin 3-galactoside (cy3-gal, fructose, sucrose, glucose and malic acid were the primary organic compounds, and 1-butanol-2-methyl-acetate, 2-hexenal and 1-hexanol were the most abundant aroma components in the skin. Furthermore, rapidly changing soluble sugars and organic acid synchronization took place in the early ripening period, while rapidly changing aroma components occurred later, on the basis of fresh weight. This result suggested that the production of aroma components might be a useful index of apple maturity.

  6. Influence of cold stress on contents of soluble sugars, vitamin C and free amino acids including gamma-aminobutyric acid (GABA) in spinach (Spinacia oleracea).

    Science.gov (United States)

    Yoon, Young-Eun; Kuppusamy, Saranya; Cho, Kye Man; Kim, Pil Joo; Kwack, Yong-Bum; Lee, Yong Bok

    2017-01-15

    The contents of soluble sugars (sucrose, fructose, glucose, maltose and raffinose), vitamin C and free amino acids (34 compounds, essential and non-essential) were quantified in open-field and greenhouse-grown spinaches in response to cold stress using liquid chromatography. In general, greenhouse cultivation produced nutritionally high value spinach in a shorter growing period, where the soluble sugars, vitamin C and total amino acids concentrations, including essential were in larger amounts compared to those grown in open-field scenarios. Further, low temperature exposure of spinach during a shorter growth period resulted in the production of spinach with high sucrose, ascorbate, proline, gamma-aminobutyric acid, valine and leucine content, and these constitute the most important energy/nutrient sources. In conclusion, cultivation of spinach in greenhouse at a low temperature (4-7°C) and exposure for a shorter period (7-21days) before harvest is recommended. This strategy will produce a high quality product that people can eat. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Growth responses and accumulation of soluble sugars in Inga marginata Wild. (Fabaceae) subjected to flooding under contrasting light conditions.

    Science.gov (United States)

    Bender, B; Capellesso, E S; Lottici, M E; Sentkovski, J; Mielniczki-Pereira, A A; Rosa, L M G; Sausen, T L

    2017-01-01

    Flood events in riparian forests of southern Brazil, can be characterized as unpredictable and of low magnitude with an average duration of less than 15 days. Inga marginata is an evergreen tree which grows in Southeast South America on a wide range of environments, including riparian forests. In this paper, the interactive effects of the light environment and soil flooding on morphological parameters of I. marginata were examined. Seedlings were acclimated in two contrasting light conditions: sun or shade for 30 days. Sun and shade plants were subjected to soil flooding for two periods; five or 15 days. After 5 days, the interaction between flooding and light did not affect growth, chlorophyll content and dry mass or the root-shoot ratio. After 15 days, flooded plants from the sunny treatment had a lower shoot dry mass compared to control sun plants and flooded plants from the shaded treatment. Moreover, the higher dry mass observed for shade plants compared to sun plants, following flooding, can also be directly associated with a higher content of soluble sugars. Shade plants of I. marginata showed a greater acclimation to soil waterlogging. This acclimation appears to be associated with a larger accumulation of soluble sugars compared to non-flooded plants. The responses observed on the shade plants appear to be decisive to indicate the use of I. marginata in degraded areas.

  8. A physicochemical study of sugar palm (Arenga Pinnata) starch films plasticized by glycerol and sorbitol

    Science.gov (United States)

    Poeloengasih, Crescentiana D.; Pranoto, Yudi; Hayati, Septi Nur; Hernawan, Rosyida, Vita T.; Prasetyo, Dwi J.; Jatmiko, Tri H.; Apriyana, Wuri; Suwanto, Andri

    2016-02-01

    The present work explores the physicochemical characteristics of sugar palm starch film for a potential hard capsule purpose. Sugar palm (Arenga pinnata) starch films were plasticized with glycerol or sorbitol in various concentrations (30% up to 50% w/w starch). Their effects on physicochemical properties of the films were investigated. The results showed that sugar palm starch was successfully developed as the main material of film using casting method. Incorporation of both glycerol or sorbitol affected the properties of films in different ways. It was found that thickness and solubility increased as plasticizer concentration increased, whereas retraction ratio, swelling degree and swelling thickness decreased with the increased plasticizer concentration.

  9. A tonoplast sugar transporter underlies a sugar accumulation QTL in watermelon

    Science.gov (United States)

    The molecular mechanism controlling accumulation of soluble sugars in watermelon (Citrullus lanatus) fruit, a trait associated with sweet-dessert watermelon domestication, is still unknown. We re-sequenced 96 recombinant inbred lines, derived from a cross between sweet and unsweet watermelon accessi...

  10. Frost decreases content of sugars, ascorbic acid and some quercetin glycosides but stimulates selected carotenes in Rosa canina hips.

    Science.gov (United States)

    Cunja, Vlasta; Mikulic-Petkovsek, Maja; Zupan, Anka; Stampar, Franci; Schmitzer, Valentina

    2015-04-15

    Primary and secondary metabolites of Rosa canina hips were determined by HPLC/MS during ripening and after frost damage. Rose hips were harvested six times from the beginning of September until the beginning of December. Color parameters a*, b* and L* decreased during maturation. Glucose and fructose were the predominant sugars representing up to 92% total sugars, and citric acid was the major organic acid detected in rose hips (constituting up to 58% total organic acids). Total sugar and ascorbic acid content significantly decreased after frost damage; from 42.2 to 25.9 g 100 g(-1) DW for sugars and from 716.8 to 176.0 mg 100 g(-1) DW for ascorbic acid. Conversely, β-carotene and lycopene levels increased in frostbitten rose hips to 22.1 and 113.2 mg 100 g(-1) DW, respectively. In addition to cyanidin-3-glucoside (highest level in hips was 125.7 μg 100 g (-1) DW), 45 different phenolic compounds have been identified. The most abundant were proanthocyanidins (their levels amounted up to 90% of total flavanol content) and their content showed no significant differences during maturation. The levels of catechin, phloridzin, flavanones and several quercetin glycosides were highest on the first three sampling dates and decreased after frost. Antioxidant capacity similarly decreased in frostbitten rose hips. Total phenolic content increased until the third sampling and decreased on later samplings. Copyright © 2015 Elsevier GmbH. All rights reserved.

  11. Sugars and Desiccation Tolerance in Seeds 1

    Science.gov (United States)

    Koster, Karen L.; Leopold, A. Carl

    1988-01-01

    Soluble sugars have been shown to protect liposomes and lobster microsomes from desiccation damage, and a protective role has been proposed for them in several anhydrous systems. We have studied the relationship between soluble sugar content and the loss of desiccation tolerance in the axes of germinating soybean (Glycine max L. Merr. cv Williams), pea (Pisum sativum L. cv Alaska), and corn (Zea mays L. cv Merit) axes. The loss of desiccation tolerance during imbibition was monitored by following the ability of seeds to germinate after desiccation following various periods of preimbibition and by following the rates of electrolyte leakage from dried, then rehydrated axes. Finally, we analyzed the soluble sugar contents of the axes throughout the transition from desiccation tolerance to intolerance. These analyses show that sucrose and larger oligosaccharides were consistently present during the tolerant stage, and that desiccation tolerance disappeared as the oligosaccharides were lost. The results support the idea that sucrose may serve as the principal agent of desiccation tolerance in these seeds, with the larger oligosaccharides serving to keep the sucrose from crystallizing. PMID:16666392

  12. Increase The Sugar Concentration of The Solution Sugar by Reverse Osmotic Membrane

    Science.gov (United States)

    Redjeki, S.; Hapsari, N.; Iriani

    2018-01-01

    Sugar is one of the basic needs of people and food and drink industry. As technology advances and the demand for efficient usage of sugar rises, crystal sugar is seen as less advantageous than liquid sugar. If sugar is always dissolved in water before use, then it will be more efficient and practical for consumers to use sugar in liquid form than in crystal form. Other than that, liquid sugar is also attractive to consumers because it is economical, hygienic, instantly soluble in hot and cold water, fresher and longer-lasting, able to thicken and enrich the texture of foods and drinks, and functions as sweetener, syrup, and flavor enhancer. Liquid sugar is also more beneficial for sugar producers because of simpler production process, cheaper production cost, and similar yield with no extra cost. In sugar production, separation process is found in most of its stages and therefore the use of membrane technology for separating solute and water content has a good potential. In this research, water content reduction of sugar solution was done in order to increase the sugar concentration of the solution. The parameters of this research were 4%, 5%, and 6% starting concentration of sugar solution; 20, 40, and 60 minutes of process time; and 85 and 60 PSI ΔP. The best result was acquired on 4% starting concentration, 60 PSI ΔP, and 60 minutes process time.

  13. Changes in soluble carbohydrates during storage of Caesalpinia echinata LAM. (Brazilwood seeds, an endangered leguminous tree from the Brazilian Atlantic Forest

    Directory of Open Access Journals (Sweden)

    I. S. Garcia

    Full Text Available Caesalpinia echinata seeds stored in laboratory environmental conditions lose their viability in one month whilst under low temperatures germination is maintained for 18 months of storage. These seeds are tolerant to desiccation, keeping their viability up to 0.08 gH2O.gDW-1. Since soluble carbohydrates are believed to be involved with desiccation tolerance and seed storability, the aim of this work is to analyze the content and composition of soluble carbohydrates in C. echinata seeds during storage in paper bags (PB and glass flasks (GF at laboratory room (RT and cool (CT temperatures. In freshly harvested seeds, total soluble carbohydrates comprised approximately 10% of the dry weight, decreasing to ca. 8% over 18 months of storage at RT. In seeds stored at CT, sugars varied differently decreasing initially and being restored at the end of the analysis period. The main neutral sugars in seeds from all treatments were sucrose, fructose and glucose. Raffinose and stachyose were present as traces. Free myo-inositol and other cyclitols were also detected. The main tendency observed was the variation in levels of both glucose and fructose in relation to sucrose, the highest levels of monosaccharides which were found in seeds stored at CT. The values of glucose and fructose were practically constant in seeds stored in paper bags for 18 months at CT, decreasing consistently in the other treatments, mainly at RT. Sucrose contents remained relatively stable. Changes in soluble sugars during storage suggest that the loss of germinability of seeds of C. echinata could be associated with low levels of glucose and fructose in relation to sucrose.

  14. Soluble carbohydrates in cereal (wheat, rye, triticale seed after storage under accelerated ageing conditions

    Directory of Open Access Journals (Sweden)

    Agnieszka I. Piotrowicz-Cieślak

    2011-01-01

    Full Text Available Germinability and the content of soluble carbohydrates were analysed in cereal seed (winter rye, cv. Warko; spring wheat, cv. Santa; hexaploid winter triticale, cv. Fidelio and cv. Woltario. Seed moisture content (mc was equilibrated over silica gel to 0.08 g H2O/g dry mass and stored in a desiccator at 20oC for up to 205 weeks or were equilibrated to mc 0.06, 0.08 or 0.10 g H2O/g dm and subjected to artificial aging at 35oC in air-tight laminated aluminium foil packages for 205 weeks. It was shown that the rate of seed aging depended on the species and seed moisture content. The fastest decrease of germinability upon storage was observed in seed with the highest moisture level. Complete germinability loss for winter rye, winter triticale cv. Fidelio, winter triticale cv. Woltario and spring wheat seed with mc 0.10 g H2O/g dm3 occurred after 81, 81, 101 and 133 weeks, respectively. Fructose, glucose, galactose, myo-inositol, sucrose, galactinol, raffinose, stachyose and verbascose were the main soluble carbohydrates found in the seed. The obtained data on the contents of specific sugars and the composition of soluble sugars fraction in seed of rye, wheat and triticale did not corroborate any profound effect of reducing sugars, sucrose and oligosaccharides on seed longevity.

  15. Seasonal alteration of sugar metabolism in strawberry ( Fragaria ...

    African Journals Online (AJOL)

    Plants of strawberry cvs Aromas and Diamante were removed from the field in cold acclimated (CA, January) and non-acclimated (NA, July) stages. Crown parts of the plant were used for analysis. Apoplastic total soluble sugar (TSS), reducing sugars and sucrose contents did not change in both cultivars in both sampling ...

  16. Accumulation of soluble sugars in peel at high temperature leads to stay-green ripe banana fruit.

    Science.gov (United States)

    Yang, Xiaotang; Pang, Xuequn; Xu, Lanying; Fang, Ruiqiu; Huang, Xuemei; Guan, Peijian; Lu, Wangjin; Zhang, Zhaoqi

    2009-01-01

    Bananas (Musa acuminata, AAA group) fail to develop a yellow peel and stay green when ripening at temperatures >24 degrees C. The identification of the mechanisms leading to the development of stay-green ripe bananas has practical value and is helpful in revealing pathways involved in the regulation of chlorophyll (Chl) degradation. In the present study, the Chl degradation pathway was characterized and the progress of ripening and senescence was assessed in banana peel at 30 degrees C versus 20 degrees C, by monitoring relevant gene expression and ripening and senescence parameters. A marked reduction in the expression levels of the genes for Chl b reductase, SGR (Stay-green protein), and pheophorbide a oxygenase was detected for the fruit ripening at 30 degrees C, when compared with fruit at 20 degrees C, indicating that Chl degradation was repressed at 30 degrees C at various steps along the Chl catabolic pathway. The repressed Chl degradation was not due to delayed ripening and senescence, since the fruit at 30 degrees C displayed faster onset of various ripening and senescence symptoms, suggesting that the stay-green ripe bananas are of similar phenotype to type C stay-green mutants. Faster accumulation of high levels of fructose and glucose in the peel at 30 degrees C prompted investigation of the roles of soluble sugars in Chl degradation. In vitro incubation of detached pieces of banana peel showed that the pieces of peel stayed green when incubated with 150 mM glucose or fructose, but turned completely yellow in the absence of sugars or with 150 mM mannitol, at either 20 degrees C or 30 degrees C. The results suggest that accumulation of sugars in the peel induced by a temperature of 30 degrees C may be a major factor regulating Chl degradation independently of fruit senescence.

  17. Cellular and soluble components decrease the viable pathogen counts in milk from dairy cows with subclinical mastitis.

    Science.gov (United States)

    Koshiishi, Tomoko; Watanabe, Masako; Miyake, Hajime; Hisaeda, Keiichi; Isobe, Naoki

    2017-08-10

    The present study was undertaken to clarify the factors that reduce the viable pathogen count in milk collected from the udders of subclinical mastitic cows during preservation. Milk was centrifuged to divide somatic cells (cellular components, precipitates) and antimicrobial peptides (soluble components, supernatants without fat layer); each fraction was cultured with bacteria, and the number of viable bacteria was assessed prior to and after culture. In 28.8% of milk samples, we noted no viable bacteria immediately after collection; this value increased significantly after a 5-hr incubation of milk with cellular components but not with soluble components (48.1 and 28.8%, respectively). After culture with cellular components, the numbers of bacteria (excluding Staphylococcus aureus and Streptococcus uberis) and yeast decreased dramatically, although the differences were not statistically significant. After cultivation with soluble components, only yeasts showed a tendency toward decreased mean viability, whereas the mean bacterial counts of S. uberis and T. pyogenes tended to increase after 5-hr preservation with soluble components. These results suggest that most pathogens in high somatic cell count (SCC) milk decreased during preservation at 15 to 25°C, due to both the cellular components and antimicrobial components in the milk. Particularly, the cellular components more potently reduced bacterial counts during preservation.

  18. The influence of growth conditions and duckweed diversity on the content of soluble sugar%生长条件和多样性对浮萍可溶性糖含量的影响

    Institute of Scientific and Technical Information of China (English)

    李阳; 成家杨; 钟钰; 唐杰

    2016-01-01

    To explore the effects of growth conditions and duckweed species diversity on duckweed growth and soluble sugar productivity,three strains (Lemna aequinoctialis LC33,Landoltia punctata LC06 and Spirodela polyrhiza LC15) were employed to culture either in polyculture or in monocultures under different conditions (temperature,light intensity,nutrient concentration).Results showed that the duckweed accumulated more soluble content and biomass under the low temperature (20 ℃),high light intensity (10 000 lx) and lower nutrient concentration (0 mg N/L,0 mg P/L).Overall,several duckweed polyculture had higher soluble sugar content than those in monoculture.Specifically,the soluble sugar content of the poluculture composed of three strains was the highest when the temperature was low;the soluble sugar content of the polycultures composed of two or three strains was higher than those in monoculture when the light intensity was high;the polyculture composed of two strains accumulated more soluble content under the low nutrient concentration.Therefore,the growth conditions and species diversity had a great influence on soluble sugar content of duckweed.The appropriate polyculture is helpful for improving the biomass production of the whole duckweed cultivation system.%以Lemna aequinoctialis LC33,Landoltia punc tataLC06和Spirodela polyrhiza LC15作为供试材料,在不同环境条件(温度、光照、营养)下对其进行单独和混合培养,研究生长条件和物种多样性对可溶性糖含量和生物量的影响.结果表明,低温(20℃)、强光照(10000lx)、低营养(N为0mg/L,P为0mg/L)的条件更有利于浮萍可溶性糖的积累.此外,适当的混养有助于浮萍可溶性糖的积累,如由3种浮萍组成的混养体系在低温条件下可溶性糖含量较高;由两种或3种浮萍组成的混养体系在强光照条件下可溶性糖含量较高;由两种浮萍组成的混养体系在低营养条件下可溶性糖含量较高.由此可见,生长条件和物种多样性与可溶性糖含量密切相关,适当的混合培养可以提高整个体系的生物质产量.

  19. Dynamic analysis of sugar metabolism in different harvest seasons ...

    African Journals Online (AJOL)

    In pineapple fruits, sugar accumulation plays an important role in flavor characteristics, which varies according to the stage of fruit development. Metabolic changes in the contents of fructose, sucrose and glucose and reducing sugar related to the activities of soluble acid invertase (AI), neutral invertase (NI), sucrose ...

  20. Variation in total sugars and reductive sugars in the moss Pleurozium schreberi (hylocomiaceae) under water deficit conditions

    International Nuclear Information System (INIS)

    Montenegro Ruiz, Luis Carlos; Melgarejo Munoz, Luz Marina.

    2012-01-01

    The structural simplicity of the bryophytes exposed them easily to water stress, forcing them to have physiological and biochemical mechanisms that enable them to survive. This study evaluated the variation of total soluble sugars and reducing sugars in relation to relative water content, in Pleurozium schreberi when faced with low water content in the Paramo de Chingaza (Colombia) and under simulated conditions of water deficit in the laboratory. we found that total sugars increase when the plant is dehydrated and returned to their normal content when re-hydrated moss, this could be interpreted as a possible mechanism of osmotic adjustment and osmoprotection of the cell content and cellular structure. Reducing sugars showed no significant variation, showing that monosaccharides do not have a protective role during dehydration.

  1. Vacuolar invertase gene silencing in potato (Solanum tuberosum L. improves processing quality by decreasing the frequency of sugar-end defects.

    Directory of Open Access Journals (Sweden)

    Xiaobiao Zhu

    Full Text Available Sugar-end defect is a tuber quality disorder and persistent problem for the French fry processing industry that causes unacceptable darkening of one end of French fries. This defect appears when environmental stress during tuber growth increases post-harvest vacuolar acid invertase activity at one end of the tuber. Reducing sugars produced by invertase form dark-colored Maillard reaction products during frying. Acrylamide is another Maillard reaction product formed from reducing sugars and acrylamide consumption has raised health concerns worldwide. Vacuolar invertase gene (VInv expression was suppressed in cultivars Russet Burbank and Ranger Russet using RNA interference to determine if this approach could control sugar-end defect formation. Acid invertase activity and reducing sugar content decreased at both ends of tubers. Sugar-end defects and acrylamide in fried potato strips were strongly reduced in multiple transgenic potato lines. Thus vacuolar invertase silencing can minimize a long-standing French fry quality problem while providing consumers with attractive products that reduce health concerns related to dietary acrylamide.

  2. Decreased levels of soluble Toll-like Receptor 2 in patients with asthma

    Directory of Open Access Journals (Sweden)

    Mohsen Tehrani

    2012-10-01

    Full Text Available Background: Recently, reports have indicated a role for the membrane form of Toll-like Receptor 2 (TLR2 in asthma pathogenesis. In this study we examined soluble TLR2 levels in serum and sputum of asthmatic and healthy subjects. Methods: Serum and sputum samples were obtained from 33 asthmatic and 19 healthy subjects. The asthmatics were classified into four groups according to the Global Initiative for Asthma. A sandwich ELISA was developed to measure soluble TLR2 (sTLR2 in serum and sputum. TLR2 mRNA expression was determined by semi-quantitative RT-PCR of all sputum samples. Results: The mean sTLR2 levels from serum and sputum of asthmatics were significantly lower than those from healthy subjects. Moreover, sTLR2 concentration decreased concomitantly with asthma severity. The differences observed, however, were not statistically significant. TLR2/GAPDH mRNA of sputum leukocytes was also significantly lower in asthmatics than in healthy subjects. Conclusion: This study demonstrated for the first time thatsTLR2 levels are lower in serum and sputum samples from asthmatic than from healthy subjects, and this could be an indicator of TLR2 expression. We also found that sTLR2 concentration in serum decreased concomitantly with an increase of asthma severity clinical score.

  3. Effects of photoperiod, growth temperature and cold acclimatisation on glucosinolates, sugars and fatty acids in kale.

    Science.gov (United States)

    Steindal, Anne Linn Hykkerud; Rødven, Rolf; Hansen, Espen; Mølmann, Jørgen

    2015-05-01

    Curly kale is a robust, cold tolerant plant with a high content of health-promoting compounds, grown at a range of latitudes. To assess the effects of temperature, photoperiod and cold acclimatisation on levels of glucosinolates, fatty acids and soluble sugars in kale, an experiment was set up under controlled conditions. Treatments consisted of combinations of the temperatures 15/9 or 21/15 °C, and photoperiods of 12 or 24h, followed by a cold acclimatisation period. Levels of glucosinolates and fatty acid types in leaves were affected by growth conditions and cold acclimatisation, being generally highest before acclimatisation. The effects of growth temperature and photoperiod on freezing tolerance were most pronounced in plants grown without cold acclimatisation. The results indicate that cold acclimatisation can increase the content of soluble sugar and can thereby improve the taste, whilst the content of unsaturated fatty and glucosinolates acids may decrease. Copyright © 2014 Elsevier Ltd. All rights reserved.

  4. Effects of limited irrigation on root yield and quality of sugar beet ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-12-17

    Dec 17, 2008 ... Irrigation treatments had a significant effect on sugar yield and its quality. Potassium ... beet plants respond to water stress by an increase in tap- ..... nitrogen fertilization on soluble carbohydrate concentration in sugar beet.

  5. Effect of electron radiation on sugar content in inverted liquid sugar

    International Nuclear Information System (INIS)

    Podadera, P.; Sabato, S.F.

    2009-01-01

    Inverted liquid sugar is a mixture of sucrose, glucose and fructose, which shows its relevant characteristic on high sweetness power. Ionizing radiation has been applied to different kind of food and ingredients for different reasons, such as pathogens reduction, disinfestations, quarantine purposes, ripening delay among others. Radiation from an electron beam can be utilized as a technique to treat this ingredient because it can process a great volume of material per unit of time. The main goal of this paper was to verify the effect of radiation on the properties of inverted liquid sugar. This ingredient was irradiated in an electron accelerator (Radiation Dynamics) at a dose ranging from 5 to 50 kGy. Sucrose content measurements were reduced by 23% at 30 kGy when compared to control and the reduced sugar content increased around 11%. Density and moisture values were not affected by radiation. The total soluble solids (Brix degrees) rose in function of the absorbed dose. (authors)

  6. Comparative assessment of sugar and malic acid composition in cultivated and wild apples.

    Science.gov (United States)

    Ma, Baiquan; Chen, Jie; Zheng, Hongyu; Fang, Ting; Ogutu, Collins; Li, Shaohua; Han, Yuepeng; Wu, Benhong

    2015-04-01

    Soluble sugar and malic acid contents in mature fruits of 364 apple accessions were quantified using high-performance liquid chromatography (HPLC). Fructose and sucrose represented the major components of soluble sugars in cultivated fruits, whilst fructose and glucose were the major items of sugars in wild fruits. Wild fruits were significantly more acidic than cultivated fruits, whilst the average concentration of total sugars and sweetness index were quite similar between cultivated and wild fruits. Thus, our study suggests that fruit acidity rather than sweetness is likely to have undergone selection during apple domestication. Additionally, malic acid content was positively correlated with glucose content and negatively correlated with sucrose content. This suggests that selection of fruit acidity must have an effect on the proportion of sugar components in apple fruits. Our study provides information that could be helpful for future apple breeding. Copyright © 2014 Elsevier Ltd. All rights reserved.

  7. Removal of Water-Soluble Extractives Improves the Enzymatic Digestibility of Steam-Pretreated Softwood Barks.

    Science.gov (United States)

    Frankó, Balázs; Carlqvist, Karin; Galbe, Mats; Lidén, Gunnar; Wallberg, Ola

    2018-02-01

    Softwood bark contains a large amounts of extractives-i.e., soluble lipophilic (such as resin acids) and hydrophilic components (phenolic compounds, stilbenes). The effects of the partial removal of water-soluble extractives before acid-catalyzed steam pretreatment on enzymatic digestibility were assessed for two softwood barks-Norway spruce and Scots pine. A simple hot water extraction step removed more than half of the water-soluble extractives from the barks, which improved the enzymatic digestibility of both steam-pretreated materials. This effect was more pronounced for the spruce than the pine bark, as evidenced by the 30 and 11% glucose yield improvement, respectively, in the enzymatic digestibility. Furthermore, analysis of the chemical composition showed that the acid-insoluble lignin content of the pretreated materials decreased when water-soluble extractives were removed prior to steam pretreatment. This can be explained by a decreased formation of water-insoluble "pseudo-lignin" from water-soluble bark phenolics during the acid-catalyzed pretreatment, which otherwise results in distorted lignin analysis and may also contribute to the impaired enzymatic digestibility of the barks. Thus, this study advocates the removal of extractives as the first step in the processing of bark or bark-rich materials in a sugar platform biorefinery.

  8. "JCE" Classroom Activity #105. A Sticky Situation: Chewing Gum and Solubility

    Science.gov (United States)

    Montes-Gonzalez, Ingrid; Cintron-Maldonado, Jose A.; Perez-Medina, Ilia E.; Montes-Berrios, Veronica; Roman-Lopez, Saurie N.

    2010-01-01

    In this Activity, students perform several solubility tests using common food items such as chocolate, chewing gum, water, sugar, and oil. From their observations during the Activity, students will initially classify the substances tested as soluble or insoluble. They will then use their understanding of the chemistry of solubility to classify the…

  9. Anomalous dissolution behaviour of tablets prepared from sugar glass-based solid dispersions

    NARCIS (Netherlands)

    Van Drooge, D.J.; Hinrichs, W.L.J.; Frijlink, H.W.

    2004-01-01

    In this study, anomalous dissolution behaviour of tablets consisting of sugar glass dispersions was investigated. The poorly aqueous soluble diazepam was used as a lipophilic model drug. The release of diazepam and sugar carrier was determined to study the mechanisms governing dissolution behaviour.

  10. Rapid screening for anthocyanins in cane sugars using ESR spectroscopy.

    Science.gov (United States)

    Thamaphat, Kheamrutai; Goodman, Bernard A; Limsuwan, Pichet; Smith, Siwaporn Meejoo

    2015-03-15

    Anthocyanin, which is soluble in water and released into sugar steam during extraction, was investigated in this study. The anthocyanin content in refined sugar, plantation white sugar, soft brown sugar and raw sugar was determined using electron spin resonance (ESR) spectroscopy, which was operated at room temperature, and compared with spectra from standard anthocyanin. The ESR spectra of red and violet anthocyanins was predominantly g ≈ 2.0055, which corresponded to an unpaired electron located in the pyrylium ring. Signals for Fe(III) and Mn(II), which naturally occur in plants, were found in raw sugar, soft brown sugar and standard anthocyanin but were absent from refined sugar and plantation white sugar due to the refining process. In addition, the ESR results were correlated with the apparent colour of the sugar, which was determined using the method of the International Commission for Uniform Methods of Sugar Analysis and inductively coupled plasma optical emission spectroscopy. Copyright © 2014 Elsevier Ltd. All rights reserved.

  11. Physicochemical and sensory characteristics of reduced sugar starfruit juice

    Directory of Open Access Journals (Sweden)

    Prasetijio, L.D.,

    2017-06-01

    Full Text Available The objective of this research was to study the physicochemical and sensory characteristics of reduced sugar starfruit juice. Cloudy starfruit juice produced by juicing of the edible part of starfruit, then the obtained filtrate was formulated with citric acid, Na- CMC and different ratio of cane sugar and sorbitol i.e. 10.0%:0.0%, 7.5%:2.5%, 5.0%:5.0%, 2.5%:5.0% and 0.0%:10.0% (% b/v. Physicochemical characteristics of the starfruit juice were total soluble solids in a range of 9.20 and 11.90%, the viscosity of 8.27 and 8.65 cP, pH of 3.56 and 3.63; and titratable acidity of 0.29 and 0.31%. Those cane sugar and sorbitol ratios affected significantly only on the total soluble solids. Based on the sensory evaluation, starfruit juice with cane sugar and sorbitol ratio of 7.50%:2.50% had the highest total preference scores of color, appearance, and taste. Colloidal stability of the starfruit juices during 10 days storage was in a range of 86.26% and 88.18%.

  12. Effectiveness of anchovy substrate application on decreasing acid solubility of Sprague Dawley rats’ tooth enamel (in vivo)

    Science.gov (United States)

    Triputra, F.; Puspitawati, R.; Gunawan, H. A.

    2017-08-01

    Anchovies (Stolephorus insularis), a natural resource of Indonesia, contain fluoride in the form of CaF2 and can function as a fluoridation material to prevent dental caries. The aim of this study is to study the effectiveness of anchovy substrate, through food or topical application, in decreasing the acid solubility of tooth enamel. This research used 14 Sprague Dawley rats as subjects divided into the following 5 groups: baseline, experimental feeding, experimental smearing, and their negative controls. After 15 days of anchovy substrate application, lower incisors were extracted and the acid solubility of enamel was analyzed qualitatively and quantitatively using a stereo microscope and a Micro-Vickers Hardness Tester. Analysis of enamel surface destruction and enamel surface microscopic hardness shifting after a 60 sec application of H2PO4 (50% concentration) resulted in a decrease in acid solubility of enamel treated with anchovy substrate. This result can be seen with both the chewing and smearing method. S. insularis can be used as an alternative material for fluoridation.

  13. Physicochemical and antioxidant properties of non-refined sugarcane alternatives to white sugar

    OpenAIRE

    Seguí Gil, Lucía; CALABUIG JIMENEZ, LAURA; Betoret Valls, Noelia; Fito Maupoey, Pedro

    2015-01-01

    [EN] Antioxidant properties of commercial sugarcane-derived products were analysed to study their suitability for being used as functional ingredients. Cane honey, several jaggeries and several brown sugars were selected from the market and analysed in terms of physicochemical characteristics and antioxidant properties, and compared with white refined sugar (twelve products in total). Moisture, water activity, total soluble solids, pH, colour and sugar profile are reported. As for antioxidant...

  14. Decreasing sugar-sweetened beverage consumption in the rural adolescent population.

    Science.gov (United States)

    Delpier, Terry; Giordana, Sheri; Wedin, Bitsy M

    2013-01-01

    Adolescent consumption of sugar-sweetened beverages (SSBs) has increased drastically with detrimental effects such as weight gain, weakened bones, dental caries, and associated higher levels of type II diabetes in this population. While in the clinical setting, rural family nurse practitioner (FNP) students, using Kellogg-funded Smart Phones, screened adolescents aged 13 to 17 years for SSB consumption in the previous 24 hours. Adolescents initially were provided with a pamphlet and related oral teaching concerning SSBs by the FNP students, as well as a water bottle to encourage healthy fluid intake. Screening SSB information was loaded onto Smart Phones, which resulted in immediate access by the primary investigator sometimes even hundreds of miles distant. After 30 days, FNP students completed follow-up phone interviews to reassess SSB consumption in the previous 24 hours. Results concerning decreased SSB consumption were statistically significant. Additionally, Smart Phones were instrumental in high-speed data transfer. Both advantages and disadvantages were encountered when using this evolving technology. Copyright © 2013 National Association of Pediatric Nurse Practitioners. Published by Mosby, Inc. All rights reserved.

  15. Impact of the nutrients N and K and soluble sugars on Diabrotica speciosa (Germar) (Coleoptera, Chrysomelidae) and Agrotis ipsilon (Hufnagel) (Lepidoptera, Noctuidae) populations in potato crops, Solanum tuberosum L. (Solanaceae); Impacto dos nutrientes N e K e de acucares soluveis sobre populacoes de Diabrotica speciosa (Germar) (Coleoptera, Chrysomelidae) e Agrotis ipsilon (Hufnagel) (Lepidoptera, Noctuidae) na cultura da batata, Solanum tuberosum L. (Solanaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Azeredo, Edson Henrique de [Universidade Federal Fluminense (UFF), Pinheiral, RJ (Brazil). Pro-Reitoria de Extensao], e-mail: edsonhenrique.azeredo@bol.com.br; Lima, Eduardo [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ (Brazil). Inst. de Agronomia. Dept. de Solos; Cassino, Paulo Cesar Rodrigues [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ (Brazil). Inst. de Biologia. Centro Integrado de Manejo de Pragas C.R.G.

    2004-03-15

    Impact of the nutrients N and K and soluble sugars on Diabrotica speciosa (Germar) (Coleoptera, Chrysomelidae) and Agrotis ipsilon (Huefnagel) (Lepidoptera, Noctuidae) populations in potato crops, Solanum tuberosum L. (Solanaceae). The occurrence of Diabrotica speciosa (Germar, 1824) and Agrotis ipsilon (Huefnagel, 1767) on the potato cultivars Achat and Monalisa, influenced by nitrogen and potassium dosage, and minimum quantity of soluble sugars, was studied. The following parameters were evaluated: concentration of mineral nutrient and sugar in green leaf, senescent leaf, leaf in abscission, stem, tubercle and total plant using extracts of infusion in ethanol 80%. The largest infestation of D. speciosa larvae was on Monalisa cultivar at 150 kg.ha{sup -1} of N + K with 27.03% at P< 0,05. It was observed that the effect of the dosage of N + K in the increment of the concentration of soluble sugars increased the damages in the tubercles and stems by A. ipsilon. The infestation by these species increased to 58.82% on the Monalisa cultivar, when the nitrogen dosage increased from zero to 150 kg.ha{sup -1}, in the absence of potassium. On the other hand, high dosage of K reduced the damages by A. ipsilon on Monalisa cultivar. However, it did not influence the storage of soluble sugar. The results indicated that in Achat cultivar the accumulated soluble sugar was reduced, probably sensitized by elevation of potassic fertilization dosing, differing from Monalisa cultivar, in which the influence was by nitrogen dosing. (author)

  16. Sugar Intake, Obesity, and Diabetes in India

    Directory of Open Access Journals (Sweden)

    Seema Gulati

    2014-12-01

    Full Text Available Sugar and sweet consumption have been popular and intrinsic to Indian culture, traditions, and religion from ancient times. In this article, we review the data showing increasing sugar consumption in India, including traditional sources (jaggery and khandsari and from sugar-sweetened beverages (SSBs. Along with decreasing physical activity, this increasing trend of per capita sugar consumption assumes significance in view of the high tendency for Indians to develop insulin resistance, abdominal adiposity, and hepatic steatosis, and the increasing “epidemic” of type 2 diabetes (T2DM and cardiovascular diseases. Importantly, there are preliminary data to show that incidence of obesity and T2DM could be decreased by increasing taxation on SSBs. Other prevention strategies, encompassing multiple stakeholders (government, industry, and consumers, should target on decreasing sugar consumption in the Indian population. In this context, dietary guidelines for Indians show that sugar consumption should be less than 10% of total daily energy intake, but it is suggested that this limit be decreased.

  17. Factors associated with the acceptance of sugar and sugar substitutes by the public.

    Science.gov (United States)

    Mackay, D A

    1985-09-01

    Acceptance is described in both market and sensory research terminology and recent developments in the fields of applied psychology and physiology are examined for their pertinence to public acceptance of sucrose and its substitutes. Information on the function of sucrose in foods other than beverages is presented with emphasis on salivation as an acceptance factor and attention is drawn to its possible dental significance. Distinctions are made between the sweetening and bulking properties of sucrose and sugar substitutes. Factors having a bearing on the acceptance of sweet foods and the determination of their optimal sugar content are described in detail. While major decreases in sucrose intake in the US resulted from high-fructose corn-sweetener usage in soft drinks, no evidence is yet available to suggest that the use of sugar substitutes of the intense artificial sweetener type has caused any decrease in ordinary sugar consumption. Neither is the consumption of polyols (sorbitol, mannitol, xylitol) high enough in confectionery categories to cause any discernible decrease in sugar usage. The evidence suggests not so much that sugar substitutes may have stopped the growth in sucrose usage, but that new product categories such as diet foods and "sugarless' confections may have been created. These categories were never available to fermentable carbohydrate sweeteners and equivalence in acceptance to sucrose-sweetened products was not an important factor in their growth.(ABSTRACT TRUNCATED AT 250 WORDS)

  18. Changes in the sugar content of sweet sorghum stems under natural conditions during winter in saline soil of the Yellow River Delta

    Science.gov (United States)

    Li, Ying; Yuan, Fang; Wang, Baoshan

    2018-02-01

    In order to investigate the maximum storage period during their natural growth state, the sweet sorghum (Sorghum bicolor L. Moench) stems of four cultivars were analyzed to determine changes in contents of water, total sugars, main soluble sugars and the enzyme activity. From early November 2016 to late January 2017, the decrease in the total sugar content and the contents of sucrose, glucose and fructose slowed down, and the enzyme activities (sucrose synthase and sucrose phosphate synthase) involving sucrose metabolism in the stem remained stable. However, these indicators decreased significantly after the end of January 2017. Low temperatures and a dry environment were conducive to the storage of the sweet sorghum stems. During the winter (from early November 2016 to late January 2017) in northern China, the sweet sorghum plants can be stored naturally in the field via regulating sowing dates, which saves a lot of storage space and production costs for bioethanol company.

  19. Dual effect of soluble materials in pretreated lignocellulose on simultaneous saccharification and co-fermentation process for the bioethanol production.

    Science.gov (United States)

    Qin, Lei; Li, Xia; Liu, Li; Zhu, Jia-Qing; Guan, Qi-Man; Zhang, Man-Tong; Li, Wen-Chao; Li, Bing-Zhi; Yuan, Ying-Jin

    2017-01-01

    In this study, wash liquors isolated from ethylenediamine and dry dilute acid pretreated corn stover were used to evaluate the effect of soluble materials in pretreated biomass on simultaneous saccharification and co-fermentation (SSCF) for ethanol production, respectively. Both of the wash liquors had different impacts on enzymatic hydrolysis and fermentation. Enzymatic conversions of glucan and xylan monotonically decreased as wash liquor concentration increased. Whereas, with low wash liquor concentrations, xylose consumption rate, cell viability and ethanol yield were maximally stimulated in fermentation without nutrient supplementary. Soluble lignins were found as the key composition which promoted sugars utilization and cell viability without nutrient supplementary. The dual effects of soluble materials on enzymatic hydrolysis and fermentation resulted in the reduction of ethanol yield as soluble materials increased in SSCF. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. ISOLATION AND CHARACTERIZATION OF SOLUBLE POLYSACCHARIDES FROM CALAMAGROSTIS ANGUSTIFOLIA KOM

    Directory of Open Access Journals (Sweden)

    Xue-Fei Cao

    2011-06-01

    Full Text Available Sequential treatments of dewaxed Calamagrostis angustifolia Kom with water (60 ºC and 90 ºC, 70% ethanol, and 70% ethanol containing 0.2%, 1.0%, 2.0%, 4.0%, and 8.0% NaOH at a solid to liquid ratio of 1:25 (g/mL at 80 ºC for 3 h yielded 36.2% soluble polysaccharides of the dry dewaxed material. The eight polysaccharide fractions obtained were comparatively studied by sugar analysis, GPC, FT-IR, 1H and 13C-NMR, and 2D-NMR (HSQC spectroscopy. The results showed that the water-soluble polysaccharides might contain noticeable amounts of β-D-glucan, as well as some pectic substances and galactoarabinoxylan. 70% ethanol-soluble polysaccharide was mainly arabinogalactan. The five alkali-soluble hemicelluloses were mainly galactoarabinoxylans. The Ara/Xyl and Ara/Gal values of H5-H8 fractions decreased with the increment of NaOH concentration from 1.0% to 8.0%. Meanwhile, the molecular weights had a declining trend from ~60,000 to ~40,000 g/mol. The smaller sized and more branched polysaccharides tended to be extracted in the early stages under milder conditions, and the larger molecular sized and more linear hemicelluloses tended to be isolated under more highly alkaline conditions.

  1. Effects of Watering and Fertilization on Carbohydrate Reserves in Sugar Maple Seedlings

    Science.gov (United States)

    Clayton M., Jr. Carl; John R. Donnelly; Boyd W. Post

    1978-01-01

    Sugar maple seedlings, grown under three nutrient and three moisture levels, were analyzed after three growing seasons for starch and ethanol-soluble sugars. Analytical procedures are detailed in the appendix. Fertilization did not affect carbohydrate levels in stems or roots. Water stress caused a significant reduction in the amount of carbohydrates in stems and roots...

  2. Evidence that cellulolysis by an anaerobic ruminal fungus is catabolite regulated by glucose, cellobiose, and soluble starch

    International Nuclear Information System (INIS)

    Morrison, M.; Mackie, R.I.; Kistner, A.

    1990-01-01

    A Piromyces-like ruminal fungus was used to study preferential carbohydrate utilization of [U- 14 C]cellulose, both alone and in combination with several soluble sugars. For cells grown on cellulose alone, cellulolytic activity was immediate and, initially, greater than that observed in the presence of added carbohydrate. Cellulolytic activity remained minimal in cultures containing cellulose plus glucose or cellobiose until the soluble sugar was depleted. Soluble starch also regulated cellulose activity but to a lesser extent. The results presented suggest that some fungal cellulases are susceptible to catabolite regulatory mechanisms

  3. Sugar-rich sweet sorghum is distinctively affected by wall polymer features for biomass digestibility and ethanol fermentation in bagasse.

    Science.gov (United States)

    Li, Meng; Feng, Shengqiu; Wu, Leiming; Li, Ying; Fan, Chunfen; Zhang, Rui; Zou, Weihua; Tu, Yuanyuan; Jing, Hai-Chun; Li, Shizhong; Peng, Liangcai

    2014-09-01

    Sweet sorghum has been regarded as a typical species for rich soluble-sugar and high lignocellulose residues, but their effects on biomass digestibility remain unclear. In this study, we examined total 63 representative sweet sorghum accessions that displayed a varied sugar level at stalk and diverse cell wall composition at bagasse. Correlative analysis showed that both soluble-sugar and dry-bagasse could not significantly affect lignocellulose saccharification under chemical pretreatments. Comparative analyses of five typical pairs of samples indicated that DP of crystalline cellulose and arabinose substitution degree of non-KOH-extractable hemicelluloses distinctively affected lignocellulose crystallinity for high biomass digestibility. By comparison, lignin could not alter lignocellulose crystallinity, but the KOH-extractable G-monomer predominately determined lignin negative impacts on biomass digestions, and the G-levels released from pretreatments significantly inhibited yeast fermentation. The results also suggested potential genetic approaches for enhancing soluble-sugar level and lignocellulose digestibility and reducing ethanol conversion inhibition in sweet sorghum. Copyright © 2014. Published by Elsevier Ltd.

  4. Ambient aerosol concentrations of sugars and sugar-alcohols at four different sites in Norway

    Directory of Open Access Journals (Sweden)

    K. E. Yttri

    2007-08-01

    Full Text Available Sugars and sugar-alcohols are demonstrated to be important constituents of the ambient aerosol water-soluble organic carbon fraction, and to be tracers for primary biological aerosol particles (PBAP. In the present study, levels of four sugars (fructose, glucose, sucrose, trehalose and three sugar-alcohols (arabitol, inositol, mannitol in ambient aerosols have been quantified using a novel HPLC/HRMS-TOF (High Performance Liquid Chromatography in combination with High Resolution Mass Spectrometry – Time of Flight method to assess the contribution of PBAP to PM>sub>10 and PM2.5. Samples were collected at four sites in Norway at different times of the year in order to reflect the various contributing sources and the spatial and seasonal variation of the selected compounds.

    Sugars and sugar-alcohols were present at all sites investigated, underlining the ubiquity of these highly polar organic compounds. The highest concentrations were reported for sucrose, reaching a maximum concentration of 320 ng m−3 in PM10 and 55 ng m−3 in PM2.5. The mean concentration of sucrose was up to 10 times higher than fructose, glucose and the dimeric sugar trehalose. The mean concentrations of the sugar-alcohols were typically lower, or equal, to that of the monomeric sugars and trehalose. Peak concentrations of arabitol and mannitol did not exceed 30 ng m−3 in PM10, and for PM2.5 all concentrations were below 6 ng m−3.

    Sugars and sugar-alcohols were associated primarily with coarse aerosols except during wintertime at the suburban site in Elverum, where a shift towards sub micron aerosols was observed. It is proposed that this shift was due to the intensive use of wood burning for residential heating at this site during winter, confirmed by high concurrent concentrations of levoglucosan. Elevated concentrations of sugars in PM2

  5. Enantiomer Ratios of Meteoritic Sugar Derivatives

    Science.gov (United States)

    Cooper, George

    2012-01-01

    Carbonaceous meteorites contain a diverse suite of soluble organic compounds. Studies of these compounds reveal the Solar System's earliest organic chemistry. Among the classes of organic compounds found in meteorites are keto acids (pyruvic acid, etc.), hydroxy tricarboxylic acids (1), amino acids, amides, purines and pyrimidines. The Murchison and Murray meteorites are the most studied for soluble and insoluble organic compounds and organic carbon phases. The majority of (indigenous) meteoritic compounds are racemic, (i.e., their D/L enantiomer ratios are 50:50). However, some of the more unusual (non-protein) amino acids contain slightly more of one enantiomer (usually the L) than the other. This presentation focuses on the enantiomer analyses of three to six-carbon (3C to 6C) meteoritic sugar acids. The molecular and enantiomer analysis of corresponding sugar alcohols will also be discussed. Detailed analytical procedures for sugar-acid enantiomers have been described. Results of several meteorite analyses show that glyceric acid is consistently racemic (or nearly so) as expected of non-biological mechanisms of synthesis. Also racemic are 4-C deoxy sugar acids: 2-methyl glyceric acid; 2,4-dihydroxybutyric acid; 2,3-dihydroxybutyric acid (two diastereomers); and 3,4-dihydroxybutyric acid. However, a 4C acid, threonic acid, has never been observed as racemic, i.e., it possesses a large D excess. In several samples of Murchison and one of GRA 95229 (possibly the most pristine carbonaceous meteorite yet analyzed) threonic acid has nearly the same D enrichment. In Murchison, preliminary isotopic measurements of individual threonic acid enantiomers point towards extraterrestrial sources of the D enrichment. Enantiomer analyses of the 5C mono-sugar acids, ribonic, arabinonic, xylonic, and lyxonic also show large D excesses. It is worth noting that all four of these acids (all of the possible straight-chained 5C sugar acids) are present in meteorites, including the

  6. Improving the process of progressive preliming in sugar production

    OpenAIRE

    V. A. Golybin; V. A. Fedoruk; N. A. Voronkova; I. S. Bushmin

    2016-01-01

    Tasks progressive preliming are to conduct neutralization, coagulation, deposition of non-sugars, the formation of a precipitate structure. This is the first operation of physical and chemical cleaning of the raw juice in the case of it in optimum conditions enables the coagulation and transferred to precipitate a substantial portion of high-molecular non-sugars, poorly soluble calcium salts that provides the cleaning effect of 14–18%, that is, up to half of the total effect on the whole stat...

  7. Changes in sugars, acids, and volatiles during ripening of koubo [Cereus peruvianus (L.) Miller] fruits.

    Science.gov (United States)

    Ninio, Racheli; Lewinsohn, Efraim; Mizrahi, Yosef; Sitrit, Yaron

    2003-01-29

    The columnar cactus Cereus peruvianus (L.) Miller, Cactaceae (koubo), is grown commercially in Israel. The unripe fruits are green, and the color changes to violet and then to red when the fruit is fully ripe. The content of soluble sugars was found to increase 5-fold during ripening. Glucose and fructose were the main sugars accumulated in the fruit pulp, and each increased from 0.5 to 5.5 g/100 g fresh weight during ripening. The polysaccharides content decreased during ripening from 1.4 to 0.4 g/100 g fresh weight. The titratable acidity decreased and the pH increased during ripening. The major organic acid found in the fruit was malic acid, which decreased from 0.75 g/100 g fresh weight at the mature green stage to 0.355 g/100 g fresh weight in ripe fruits. Citric, succinic, and oxalic acids were found in concentrations lower than 0.07 g/100 g fresh weight. Prominent accumulation of aroma volatiles occurred toward the end of the ripening process. The main volatile found in the ripe fruit was linalool, reaching concentrations of 1.5-3.5 microg/g fresh weight.

  8. Worldwide trends in dietary sugars intake.

    Science.gov (United States)

    Wittekind, Anna; Walton, Janette

    2014-12-01

    Estimating trends in dietary intake data is integral to informing national nutrition policy and monitoring progress towards dietary guidelines. Dietary intake of sugars is a controversial public health issue and guidance in relation to recommended intakes is particularly inconsistent. Published data relating to trends in sugars intake are relatively sparse. The purpose of the present review was to collate and review data from national nutrition surveys to examine changes and trends in dietary sugars intake. Only thirteen countries (all in the developed world) appear to report estimates of sugars intake from national nutrition surveys at more than one point in time. Definitions of dietary sugars that were used include 'total sugars', 'non-milk extrinsic sugars', 'added sugars', sucrose' and 'mono- and disaccharides'. This variability in terminology across countries meant that comparisons were limited to within countries. Hence trends in dietary sugars intake were examined by country for the whole population (where data permitted), and for specific or combined age and sex subpopulations. Findings indicate that in the majority of population comparisons, estimated dietary sugars intake is either stable or decreasing in both absolute (g/d) and relative (% energy) terms. An increase in sugars intake was observed in few countries and only in specific subpopulations. In conclusion, the findings from the present review suggest that, in the main, dietary sugars intake are decreasing or stable. A consistent approach to estimation of dietary sugars intake from national nutrition surveys is required if more valid estimates of changes in dietary sugars intakes are required in the future.

  9. Antioxidative activity of water soluble polysaccharide in pumpkin fruits (Cucurbita maxima Duchesne).

    Science.gov (United States)

    Nara, Kazuhiro; Yamaguchi, Akira; Maeda, Naomi; Koga, Hidenori

    2009-06-01

    We evaluated the antioxidative activity of a water soluble polysaccharide fraction (WSP) from pumpkin fruits (Cucurbita maxima Duchesne). In the WSP, DPPH radical scavenging and superoxide dismutase-like activity increased depending on the total sugar content. Furthermore, the WSP can serve as an inhibitor of ascorbic acid oxidation. The efficacy was also affected by the total sugar content.

  10. Sevelamer does not decrease lipopolysaccharide or soluble CD14 levels but decreases soluble tissue factor, low-density lipoprotein (LDL) cholesterol, and oxidized LDL cholesterol levels in individuals with untreated HIV infection.

    Science.gov (United States)

    Sandler, Netanya G; Zhang, Xinyan; Bosch, Ronald J; Funderburg, Nicholas T; Choi, Andrew I; Robinson, Janet K; Fine, Derek M; Coombs, Robert W; Jacobson, Jeffrey M; Landay, Alan L; Douek, Daniel C; Tressler, Randall; Read, Sarah W; Wilson, Cara C; Deeks, Steven G; Lederman, Michael M; Gandhi, Rajesh T

    2014-11-15

    Abnormal levels of inflammation are associated with cardiovascular disease and mortality in human immunodeficiency virus (HIV)-infected patients. Microbial translocation, which may cause inflammation, is decreased by sevelamer in patients undergoing hemodialysis. In this single-arm study, we evaluated the effects of 8 weeks of sevelamer therapy on 36 HIV-infected subjects who were not receiving antiretroviral therapy. Sevelamer did not significantly change markers of microbial translocation, inflammation, or T-cell activation. During sevelamer treatment, however, levels of soluble tissue factor, low-density lipoprotein (LDL) cholesterol, and oxidized LDL cholesterol decreased significantly, whereas D-dimer levels increased. Thus, in this study population, sevelamer did not reduce microbial translocation but may have yielded cardiovascular benefits. NCT 01543958. © The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  11. Kinetics of sugars consumption and ethanol inhibition in carob pulp fermentation by Saccharomyces cerevisiae in batch and fed-batch cultures.

    Science.gov (United States)

    Lima-Costa, Maria Emília; Tavares, Catarina; Raposo, Sara; Rodrigues, Brígida; Peinado, José M

    2012-05-01

    The waste materials from the carob processing industry are a potential resource for second-generation bioethanol production. These by-products are small carob kibbles with a high content of soluble sugars (45-50%). Batch and fed-batch Saccharomyces cerevisiae fermentations of high density sugar from carob pods were analyzed in terms of the kinetics of sugars consumption and ethanol inhibition. In all the batch runs, 90-95% of the total sugar was consumed and transformed into ethanol with a yield close to the theoretical maximum (0.47-0.50 g/g), and a final ethanol concentration of 100-110 g/l. In fed-batch runs, fresh carob extract was added when glucose had been consumed. This addition and the subsequent decrease of ethanol concentrations by dilution increased the final ethanol production up to 130 g/l. It seems that invertase activity and yeast tolerance to ethanol are the main factors to be controlled in carob fermentations. The efficiency of highly concentrated carob fermentation makes it a very promising process for use in a second-generation ethanol biorefinery.

  12. Sugar regulation of SUGAR TRANSPORTER PROTEIN 1 (STP1) expression in Arabidopsis thaliana

    Science.gov (United States)

    Cordoba, Elizabeth; Aceves-Zamudio, Denise Lizeth; Hernández-Bernal, Alma Fabiola; Ramos-Vega, Maricela; León, Patricia

    2015-01-01

    Sugars regulate the expression of many genes at the transcriptional level. In Arabidopsis thaliana, sugars induce or repress the expression of >1800 genes, including the STP1 (SUGAR TRANSPORTER PROTEIN 1) gene, which encodes an H+/monosaccharide cotransporter. STP1 transcript levels decrease more rapidly after the addition of low concentrations of sugars than the levels of other repressed genes, such as DIN6 (DARK-INDUCED 6). We found that this regulation is exerted at the transcriptional level and is initiated by phosphorylatable sugars. Interestingly, the sugar signal that modulates STP1 expression is transmitted through a HEXOKINASE 1-independent signalling pathway. Finally, analysis of the STP1 5′ regulatory region allowed us to delimit a region of 309bp that contains the cis elements implicated in the glucose regulation of STP1 expression. Putative cis-acting elements involved in this response were identified. PMID:25281700

  13. Sugar-starvation-induced changes of carbon metabolism in excised maize root tips

    International Nuclear Information System (INIS)

    Dieuaide-Noubhani, M.; Canioni, P.; Raymond, P.

    1997-01-01

    Excised maize (Zea mays L.) root tips were used to study the early metabolic effects of glucose (Glc) starvation. Root tips were prelabeled with [1-13C]Glc so that carbohydrates and metabolic intermediates were close to steady-state labeling, but lipids and proteins were scarcely labeled. They were then incubated in a sugar-deprived medium for carbon starvation. Changes in the level of soluble sugars, the respiratory quotient, and the 13C enrichment of intermediates, as measured by 13C and 1H nuclear magnetic resonance, were studied to detect changes in carbon fluxes through glycolysis and the tricarboxylic acid cycle. Labeling of glutamate carbons revealed two major changes in carbon input into the tricarboxylic acid cycle: (a) the phosphoenolpyruvate carboxylase flux stopped early after the start of Glc starvation, and (b) the contribution of glycolysis as the source of acetyl-coenzyme A for respiration decreased progressively, indicating an increasing contribution of the catabolism of protein amino acids, fatty acids, or both. The enrichment of glutamate carbons gave no evidence for proteolysis in the early steps of starvation, indicating that the catabolism of proteins was delayed compared with that of fatty acids. Labeling of carbohydrates showed that sucrose turnover continues during sugar starvation, but gave no indication for any significant flux through gluconeogenesis

  14. Improved sugar beet pectin-stabilized emulsions through complexation with sodium caseinate.

    Science.gov (United States)

    Li, Xiangyang; Fang, Yapeng; Phillips, Glyn O; Al-Assaf, Saphwan

    2013-02-13

    The study investigates the complexes formed between sodium caseinate (SC) and sugar beet pectin (SBP) and to harness them to stabilize SBP emulsions. We find that both hydrophobic and electrostatic interactions are involved in the complexation. In SC/SBP mixed solution, soluble SC/SBP complexes first form on acidification and then aggregate into insoluble complexes, which disassociate into soluble polymers upon further decreasing pH. The critical pH's for the formation of soluble and insoluble complexes and disappearance of insoluble complexes are designated as pH(c), pH(φ), and pH(d), respectively. These critical pH values define four regions in the phase diagram of complexation, and SC/SBP emulsions were prepared in these regions. The results show that the stability of SBP-stabilized emulsion is greatly improved at low SC/SBP ratios and acidic pH's. This enhancement can be attributed to an increase in the amount of adsorbed SBP as a result of cooperative adsorption to sodium caseinate. Using a low ratio of SC/SBP ensured that all caseinate molecules are completely covered by adsorbed SBP chains, which eliminates possible instability induced by thermal aggregation of caseinate molecules resulting from stress acceleration at elevated temperatures. A mechanistic model for the behavior is proposed.

  15. Decreased plasma levels of factor II + VII + X correlate with increased levels of soluble cytokine receptors in patients with malaria and meningococcal infections

    DEFF Research Database (Denmark)

    Bygbjerg, I C; Hansen, M B; Rønn, A M

    1997-01-01

    The levels of coagulation factors II + VII + X and of blood platelets (thrombocytes) as well as of cytokines and soluble cytokine receptors were studied in the patients with malaria or meningococcal infections. The coagulation factors were decreased particularly in the meningococcal patients, while...... thrombocytes were lowest in the Plasmodium falciparum malaria patients. There was no correlation between factors II + VII + X and thrombocytes, but plasma levels of coagulation factors II + VII + X were found to correlate inversely with levels of soluble interleukin-2 receptor (sIL-2R) and soluble tumour...... necrosis factor-I (sTNF-RI) in patients with malaria and meningococcal infections. Elevated sIL-2R and sTNF-RI levels and decreased coagulation factors reverted to normal within 3-5 days after initiation of therapy in P. falciparum patients followed consecutively. Estimation of coagulation factors may...

  16. Production of ethanol in batch and fed-batch fermentation of soluble sugar

    International Nuclear Information System (INIS)

    Chaudhary, M.Y.; Shah, M.A.; Shah, F.H.

    1991-01-01

    Keeping in view of the demand and need for alternate energy source, especially liquid fuels and the availability of raw materials in Pakistan, we have carried out biochemical and technological studies for ethanol through fermentation of renewable substrates. Molasses and sugar cane have been used as substrate for yeast fermentation. Selected yeast were used in both batch and semi continuous fermentation of molasses. Clarified dilute molasses were fermented with different strains of Saccharomyces cerevisiae. Ethanol concentration after 64 hours batch fermentation reached 9.4% with 90% yield based on sugar content. During feed batch system similar results were obtained after a fermentation cycle of 48 hours resulting in higher productivity. Similarly carbohydrates in fruit juices and hydro lysates of biomass can be economically fermented to ethanol to be used as feed stock for other chemicals. (author)

  17. Spray Drying of High Sugar Content Foods: Improving of Product Yield and Powder Properties

    Directory of Open Access Journals (Sweden)

    Mehmet Koç

    2016-05-01

    Full Text Available Spray drying is the most preferred drying method to produce powdered food in the food industry and it is also widely used to convert sugar-rich liquid foods to a powder form. During and/or after spray drying process of sugar-rich products, undesirable situation was appeared such as stickiness, high moisture affinity (hygroscopicity and low solubility due to low molecular weight monosaccharides that found naturally in the structure. The basis of these problems was formed by low glass transition temperature of sugar-rich products. This review gives information about the difficulties in drying of sugar-rich products via spray dryer, actions need to be taken against these difficulties and drying of sugar-rich honey and fruit juices with spray drying method.

  18. Towards producing novel fish gelatin films by combination treatments of ultraviolet radiation and sugars (ribose and lactose) as cross-linking agents.

    Science.gov (United States)

    Bhat, Rajeev; Karim, A A

    2014-07-01

    Developing novel fish gelatin films with better mechanical properties than mammalian gelatin is a challenging but promising endeavor. Studies were undertaken to produce fish gelatin films by combining treatments with different sugars (ribose and lactose) followed 'by' 'and' ultraviolet (UV) radiation, as possible cross-linking agents. Increase in tensile strength and percent elongation at break was recorded, which was more significant in films without sugars that were exposed to UV radiation. Films with added ribose showed decreased solubility after UV treatment and exhibited higher swelling percentage than films with added lactose, which readily dissolved in water. FTIR spectra of all the films showed identical patterns, which indicated no major changes to have occurred in the functional groups as a result of interaction between gelatin, sugars and UV irradiation. The results of this study could be explored for commercial use, depending on industrial needs for either production of edible films or for food packaging purposes.

  19. Characterization of the Water-Soluble Fraction of Woody Biomass Pyrolysis Oils

    Energy Technology Data Exchange (ETDEWEB)

    Stankovikj, Filip; McDonald, Armando G.; Helms, Gregory L.; Olarte, Mariefel V.; Garcia-Perez, Manuel

    2017-01-31

    This paper reports a study of the chemical composition of the water soluble (WS) fraction obtained by cold water precipitation of two commercial wood pyrolysis oils (BTG and Amaron). The fraction studied accounts for between 50.3 and 51.3 wt. % of the oils. With the most common analytical techniques used today for the characterization of this fraction (KF titration, GC/MS, hydrolysable sugars and total carbohydrates), it is possible to quantify only between 45 and 50 wt. % of it. Our results confirm that most of the total carbohydrates (hydrolysable sugars and non-hydrolysable) are soluble in water. The ion chromatography hydrolysis method showed that between 11.6 and 17.3 wt. % of these oils were hydrolysable sugars. A small quantity of phenols detectable by GC/MS (between 2.5 and 3.9 wt. %) were identified. It is postulated that the unknown high molecular weight fraction (30-55 wt. %) is formed by highly dehydrated sugars rich in carbonyl groups and WS phenols. The overall content of carbonyl, carboxyl, hydroxyl and phenolic compounds in the WS fraction were quantified by titration, Folin-Ciocalteu, 31P-NMR and 1H-NMR. The WS fraction contains between 5.5 and 6.2 mmol/g of carbonyl groups, between 0.4 and 1.0 mmol/g of carboxylic acid groups, between 1.2 and 1.8 mmol/g phenolic -OH, and between 6.0 and 7.9 mmol/g of aliphatic alcohol groups. Translation into weight fractions of the WS was done by supposing surrogate structures for the water soluble phenols, carbonyl and carboxyl groups and we estimated the content of WS phenols (21-27 wt. %), carbonyl (5-14 wt.%), and carboxyl (0-4 wt.%). Together with the total carbohydrates (23-27 wt.%), this approach leads to > 90 wt. % of the WS material in the bio-oils being quantified. We speculate the larger portion of the difference between the total carbohydrates and hydrolysable sugars is the missing furanic fraction. Further refinement of the suggested methods and development of separation schemes to obtain and

  20. Effect of non-nutritive sugars to decrease the survivorship of spotted wing drosophila, Drosophila suzukii

    Science.gov (United States)

    In this study, we investigated the effects of non-nutritive sugars and sugar alcohols on the survivorship of spotted wing drosophila, Drosophila suzukii, and found erythritol and erythrose as potentially toxic to the fly. In a dose-dependent study, erythritol and erythrose significantly reduced fly ...

  1. Multicompartment analysis of the effects of fertilizing nitrogen form, quantity of potassium fertilizer and tomato variety upon tomato-fruit sugar metabolism

    International Nuclear Information System (INIS)

    Watanabe, Kazuhiko; Mori, Masato; Kubo, Yunosuke; Takeba, Tsuyoshi.

    1977-01-01

    Concerning ''streaky decay'' of tomato fruits, the sugar metabolism of tomato fruits has been studied by multicompartment analysis dividing the radioactivity into 14 C-glucose non-absorbing, ethanol soluble, carbonic acid gas and ethanol insoluble compartments. 14 C-glucose was introduced to pieces of tomato fruits about 60 days after fructification. Influence in the stage of 14 C-glucose entry into the tissue was recognized in a tomato variety affected by excess ammonia and a tomato variety affected by potassium shortage on the carbonic acid gas generation portion. The decrease of metabolism turnover from the ethanol soluble to the insoluble compartment was remarkable in the tomato variety so nutritionally treated as to be apt to cause streaky decay and the variety susceptible to it. (Mori, K.)

  2. Study the Effect of Different Phosphorus Fertilizers on Physiological Characteristic of Photosynthetic Pigments and Soluble Sugars of Safflower under Water Deficit Condition

    Directory of Open Access Journals (Sweden)

    S Heshmati

    2016-10-01

    capacity. The sub-factors were the six treatments resulting from three levels of phosphate chemical fertilizer (0, 50, and 100 kg ha-1 Triple Super Phosphate, each at two levels of Barvar-2 bio-fertilizer (with and without inoculation with Barvar-2. The applied biological fertilizer was in the type of the bacteria which release the phosphorus from soil components and neutralize the soil pH. The commercial name of the biologic fertilizer is Barvar-2. The effective gradients of the biological phosphorus fertilizer is comprised of two bacteria strains of p5 (Bacillus lentus and p13 (Pseudomonas potida with 108 cfu (colony forming units which have been screened from soil bacteria populations. The bacteria strain p5 (Bacillus lentus dissolves P from soil mineral compounds while p13 (Pseudomonas potida separates P from soil organic compounds by exerting a variety of phosphatase enzymes. Results and Discussion The results showed that the highest amount of chlorophyll a, b and total chlorophyll obtained with application of chemical phosphorus by 100 kg ha-1 Triple Super Phosphate followed by inoculation with Barvar-2 under water deficit condition at vegetation and flowering stages. At drought stress in flowering stage, use of high level of phosphorus without inoculation with Barvar-2, had highest effect in terms of increasing amount of chlorophyll a/b ratio, but at treatment of inoculation with Barvar-2, the highest amount of chlorophyll a/ b ratio obtained with low level of chemical phosphorus by 50 kg ha-1 Triple Super Phosphate. The means comparison showed that highest amount of carotenoids, fv/fm, anthocyanin, flavonoids and soluble sugars obtained with application of chemical phosphorus by 50 kg ha-1 Triple Super Phosphate without Barvar-2 under water stress condition at vegetation and flowering stages. The inoculation with Barvar-2 followed by chemical phosphorus by 50 kg ha-1 Triple Super Phosphate significantly increased the amount of anthocyanin and soluble sugars of leaf

  3. Sugar uptake and starch biosynthesis by slices of developing maize endosperm

    International Nuclear Information System (INIS)

    Felker, F.C.; Liu, Kangchien; Shannon, J.C.

    1990-01-01

    14 C-Sugar uptake and incorporation into starch by slices of developing maize (Zea mays L.) endosperm were examined and compared with sugar uptake by maize endosperm-derived suspension cultures. Rates of sucrose, fructose, and D- and L-glucose uptake by slices were similar, whereas uptake rates for these sugars differed greatly in suspension cultures. Concentration dependence of sucrose, fructose, and D-glucose uptake was biphasic (consisting of linear plus saturable components) with suspension cultures but linear with slices. These and other differences suggest that endosperm slices are freely permeable to sugars. After diffusion into the slices, sugars were metabolized and incorporated into starch. Starch synthesis, but not sugar accumulation, was greatly reduced by 2.5 millimolar p-chloromercuribenzenesulfonic acid and 0.1 millimolar carbonyl cyanide m-chlorophenylhydrazone. Starch synthesis was dependent on kernel age and incubation temperature, but not on external pH (5 through 8). Competing sugars generally did not affect the distribution of 14 C among the soluble sugars extracted from endosperm slices incubated in 14 C-sugars. Competing hexoses reduced the incorporation of 14 C into starch, but competing sucrose did not, suggesting that sucrose is not a necessary intermediate in starch biosynthesis. The bidirectional permeability of endosperm slices to sugars makes the characterization of sugar transport into endosperm slices impossible, however the model system is useful for experiments dealing with starch biosynthesis which occurs in the metabolically active tissue

  4. [Sugar Chain Construction of Functional Natural Products Using Plant Glucosyltransferases].

    Science.gov (United States)

    Mizukami, Hajime

    2015-01-01

    Plant secondary product glycosyltransferases belong to family 1 of the glycosyltransferase superfamily and mediate the transfer of a glycosyl residue from activated nucleotide sugars to lipophilic small molecules, thus affecting the solubility, stability and pharmacological activities of the sugar-accepting compounds. The biotechnological application of plant glycosyltransferases in glycoside synthesis has attracted attention because enzymatic glycosylation offers several advantages over chemical methods, including (1) avoiding the use of harsh conditions and toxic catalysts, (2) providing strict control of regio-and stereo-selectivity and (3) high efficiency. This review describes the in vivo and in vitro glycosylation of natural organic compounds using glycosyltransferases, focusing on our investigation of enzymatic synthesis of curcumin glycosides. Our current efforts toward functional characterization of some glycosyltransferases involved in the biosynthesis of iridoids and crocin, as well as in the sugar chain elongation of quercetin glucosides, are described. Finally, I describe the relationship of the structure of sugar chains and the intestinal absorption which was investigated using chemoenzymatically synthesized quercetin glycosides.

  5. Tobacco expressing pap1 increases the responses to par and uv-a by enhancing soluble sugars and flavonoids and elevating plant protections

    International Nuclear Information System (INIS)

    Sompornpailin, K.; Kanthang, S.

    2015-01-01

    Five lines of transgenic tobacco over-expressing Production of Anthocyanin Pigment 1 (PAP1) cDNA were analysis of metabolic response against the radiation and their protection of the plant under tissue culture condition. PAP1 transgenic and wild type (WT) plants were treated with the radiations of photosynthetically activate radiation (PAR) or PAR combined with UV-A. All lines of transgenic significantly increased in amounts of p-coumaric acid, naringenin apigenin more than WT under both treatments. Additional UV-A radiating to plant rose up kaempferol content in WT plant (1.5 times) and in PAP1 transgenics (1.8 times). These transgenic plants treated under both conditions had also increased anthocyanin substances (pelargonidin) with significant value after compared to WT. Content of total soluble sugar (TSS) was related to the content of total flavonoids in transgenic. PAR combined with UV-A had a lower induction of the electrolyte leakage percentage and malondialdehyde (MDA) level in the transgenic leaf tissue compared to WT tissue. The metabolic substance levels were considered on its protection of plant cells. In transgenic tissue, the enhancement of apigenin level strongly diminished the increase level of electrolyte leakage while the levels of TSS, p-coumaric acid and naringinin less affected. Moreover, the increase levels of kaempferol and pelargonidin associated with the decrease level of MDA, while the TSS level reversely responded. The PAP1 transgenic increased response of light by adaptation of their metabolites (TSS, p-coumaric acid and flavonoids) consequently enhance parameter indicating protections of the cell. (author)

  6. Sugars in atmospheric aerosols over the Eastern Mediterranean

    Science.gov (United States)

    Theodosi, Christina; Panagiotopoulos, Christos; Nouara, Amel; Zarmpas, Pavlos; Nicolaou, Panagiota; Violaki, Kalliopi; Kanakidou, Maria; Sempéré, Richard; Mihalopoulos, Nikolaos

    2017-04-01

    The role of biomass combustion and primary bio-particles in atmospheric PM10 aerosols in the Eastern Mediterranean over a two-year period was estimated by studying sugar tracers. Sugar concentrations ranged from 6 to 334 ng m-3, while their contributions to the organic carbon (OC) and water soluble organic carbon (WSOC) pools were 3 and 11%, respectively. Over the studied period, glucose and levoglucosan were the two most abundant sugars accounting equally about 25% of the total sugar concentration in PM10 aerosols whereas fructose, sucrose, and mannitol represented 18%, 15% and 10%, respectively. Primary saccharides (glucose, fructose, and sucrose) peaked at the beginning of spring (21, 17 and 15 ng m-3, respectively), indicating significant contributions of bioaerosols to the total organic aerosol mass. On the other hand, higher concentrations of anhydrosugars (burning biomass tracers including levoglucosan, mannosan and galactosan) were recorded in winter (19, 1.4 and 0.2 ng m-3, respectively) than in summer (9.1, 1.1 and 0.5 ng m-3, respectively). Levoglucosan was the dominant monosaccharide in winter (37% of total sugars) with less contribution in summer (19%) probably in relation with enhanced photochemical oxidation reactions by hydroxyl (ṡOH) radicals impacting anhydrosugars. We estimate that atmospheric oxidation by ṡOH decreases levoglucosan levels by 54% during summer. Biomass burning, based on levoglucosan observations, was estimated to contribute up to 13% to the annual average OC measured at Finokalia. Annual OC, WSOC, and carbohydrate dry deposition fluxes for the studied period were estimated to 414, 175, and 9 mg C m-2 y-1, respectively. Glucose and levoglucosan accounted for 34% and 2% of the total sugar fluxes. According to our estimations, atmospheric OC and WSOC inputs account for ˜0.70% of the carbon produced by annual primary production (PP) in the Cretan Sea. Considering the entire Mediterranean, dry deposition of OC could provide at

  7. Pretreatment of Dried Distiller Grains with Solubles by Soaking in Aqueous Ammonia and Subsequent Enzymatic/Dilute Acid Hydrolysis to Produce Fermentable Sugars.

    Science.gov (United States)

    Nghiem, Nhuan P; Montanti, Justin; Kim, Tae Hyun

    2016-05-01

    Dried distillers grains with solubles (DDGS), a co-product of corn ethanol production in the dry-grind process, was pretreated by soaking in aqueous ammonia (SAA) using a 15 % w/w NH4OH solution at a solid/liquid ratio of 1:10. The effect of pretreatment on subsequent enzymatic hydrolysis was studied at two temperatures (40 and 60 °C) and four reaction times (6, 12, 24, and 48 h). Highest glucose yield of 91 % theoretical was obtained for the DDGS pretreated at 60 °C and 24 h. The solubilized hemicellulose in the liquid fraction was further hydrolyzed with dilute H2SO4 to generate fermentable monomeric sugars. The conditions of acid hydrolysis included 1 and 4 wt% acid, 60 and 120 °C, and 0.5 and 1 h. Highest yields of xylose and arabinose were obtained at 4 wt% acid, 120 °C, and 1 h. The fermentability of the hydrolysate obtained by enzymatic hydrolysis of the SAA-pretreated DDGS was demonstrated in ethanol fermentation by Saccharomyces cerevisiae. The fermentability of the hydrolysate obtained by consecutive enzymatic and dilute acid hydrolysis was demonstrated using a succinic acid-producing microorganism, strain Escherichia coli AFP184. Under the fermentation conditions, complete utilization of glucose and arabinose was observed, whereas only 47 % of xylose was used. The succinic acid yield was 0.60 g/g total sugar consumed.

  8. GC-EI-MS identification data of neutral sugars of polysaccharides extracted from Zizyphus lotus fruit

    Directory of Open Access Journals (Sweden)

    Khaoula Mkadmini Hammi

    2018-06-01

    Full Text Available Gas chromatography coupled to mass spectrometer (GC–MS was used to identify and to quantify neutral sugars that constitute the water soluble polysaccharides from Zizyphus lotus fruit. The trimethylsilyl (TMS method was successfully used for derivatization of the monosaccharides units of extracted polysaccharides that were released by hydrolysis method. Sugars were identified based on their retention times compared with those of standards and the NIST MS Spectral Library. All sugars were quantified in TIC (Total Ion Current mode using calibration curves. Data is related to “Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity” (Mkadmini Hammi et al., 2016 [1]. Keywords: Trimethylsilyl, Derivatization, GC–MS, Neutral sugar

  9. The Effect of Ultrasonic Waves on Sugar Extraction and Mechanical Properties of Sugar Beet

    Directory of Open Access Journals (Sweden)

    K Hedayati

    2013-09-01

    Full Text Available Sugar, which can be extracted from sugar cane and sugar beet, is one of the most important ingredients of food. Conducting more research to increase the extraction efficiency of sugar is necessary due to high production of sugar beet and its numerous processing units in northern Khorasan province. In this research, the effect of temperature, time and the frequency of ultrasonic waves on mechanical properties of sugar beet and its extraction rate of sugar in moisture content of 75% were studied. In this regard, an ultrasonic bath in laboratory scale was used. The studied parameters and their levels were frequency in three levels (zero, 25 and 45 KHz, temperature in three levels (25, 50 and 70 ° C and the imposed time of ultrasonic waves in three levels (10, 20 and 30 min. Samples were prepared using planned experiments and the results were compared with control sugar beet samples. A Saccharimeter was used to measure the concenteration of sugar in samples. Two different types of probe including semi-spherical end and the other one with sharpened edges were used to measure mechanical properties. The studied parameters of frequency, temperature and time showed significant effect on sugar extraction and their resulted effect in optimized levels revealed up to 56% increase in sugar extraction compared with control samples. The obtained values of elastic modulus and shear modulus showed a decreasing trend. The obtained values of total energy of rupture, the total energy of shear, the maximum force of rupture, and the yield point of rupture showed an increasing trend. The frequency had no significant effect on the yield point of rupture and shear force.

  10. The synthesis of a water-soluble derivative of rutin as an antiradical agent

    Energy Technology Data Exchange (ETDEWEB)

    Pedriali, Carla Aparecida; Fernandes, Adjaci Uchoa [Universidade de Sao Paulo (USP), SP (Brazil). Inst. de Quimica. Dept. de Bioquimica]. E-mail: capedriali@hotmail.com; Bernusso, Leandra de Cassia; Polakiewicz, Bronislaw [Universidade de Sao Paulo (USP), SP (Brazil). Faculdade de Ciencias Farmaceuticas. Dept. de Tecnologia Bioquimico-Farmaceutica

    2008-07-01

    The purpose of this study was to synthesize a water-soluble derivative of rutin (compound 2) by introducing carboxylate groups on rutin's sugar moiety. The rutin derivative showed an almost 100-fold solubility increase in water. The antiradical capacity of compound 2 was evaluated using the luminol/AAPH system, and the derivative's activity was 1.5 times greater than that of Trolox. Despite the derivative's high solubility in water (log P = -1.13), lipid peroxidation of brain homogenate membranes was very efficiently inhibited (inhibition values were only 19% lower than the inhibition values of rutin). (author)

  11. The synthesis of a water-soluble derivative of rutin as an antiradical agent

    International Nuclear Information System (INIS)

    Pedriali, Carla Aparecida; Fernandes, Adjaci Uchoa; Bernusso, Leandra de Cassia; Polakiewicz, Bronislaw

    2008-01-01

    The purpose of this study was to synthesize a water-soluble derivative of rutin (compound 2) by introducing carboxylate groups on rutin's sugar moiety. The rutin derivative showed an almost 100-fold solubility increase in water. The antiradical capacity of compound 2 was evaluated using the luminol/AAPH system, and the derivative's activity was 1.5 times greater than that of Trolox. Despite the derivative's high solubility in water (log P = -1.13), lipid peroxidation of brain homogenate membranes was very efficiently inhibited (inhibition values were only 19% lower than the inhibition values of rutin). (author)

  12. Spray Drying of High Sugar Content Foods: Improving of Product Yield and Powder Properties

    OpenAIRE

    Mehmet Koç; Figen Kaymak-Ertekin

    2016-01-01

    Spray drying is the most preferred drying method to produce powdered food in the food industry and it is also widely used to convert sugar-rich liquid foods to a powder form. During and/or after spray drying process of sugar-rich products, undesirable situation was appeared such as stickiness, high moisture affinity (hygroscopicity) and low solubility due to low molecular weight monosaccharides that found naturally in the structure. The basis of these problems was formed by low glass transiti...

  13. Current knowledge on agarolytic enzymes and the industrial potential of agar-derived sugars.

    Science.gov (United States)

    Yun, Eun Ju; Yu, Sora; Kim, Kyoung Heon

    2017-07-01

    Agar is a major cell wall carbohydrate of red macroalgae (Rhodophyta). Sugars derived from agar, such as agarooligosaccharides (AOSs), neoagarooligosaccharides (NAOSs), neoagarobiose (NAB), and 3,6-anhydro-L-galactose (L-AHG), possess various physiological activities. These agar-derived sugars can be produced by hydrolysis using chemicals or agarolytic enzymes. Despite the industrial potential of agar-derived sugars, their application has been hampered mainly due to the absence of efficient processes for the liquefaction and saccharification of agar. In this review, we have focused on strategies for producing high value-added sugars from agarose via chemical or enzymatic liquefaction and enzymatic saccharification. The liquefaction of agarose is a key step for preventing gelling and increasing the solubility of agarose in water by prehydrolyzing agarose into AOSs or NAOSs. For the industrial use of agar-derived sugars, AOS, NAOS, NAB, and L-AHG can be used as functional biomaterials owing to their physiological activities such as antiinflammation, skin whitening, and moisturizing. Recently, it was reported that AHG could be considered as a new anticariogenic sugar to replace xylitol. This review provides a comprehensive overview of processes for the hydrolysis of agar or agarose to produce high value-added sugars and the industrial application of these sugars.

  14. Soluble CD206 plasma levels in rheumatoid arthritis reflect decrease in disease activity

    DEFF Research Database (Denmark)

    Heftdal, Line Dam; Stengaard-Pedersen, Kristian; Ørnbjerg, Lykke Midtbøll

    2017-01-01

    internalization and degradation. The soluble form has been suggested as a biomarker of M2A-macrophage activation. The aim of this study was to investigate sCD206 plasma levels in early RA patients initiating anti-TNFα treatment. Plasma levels of sCD206 were measured by ELISA in samples from 155 early RA patients...... from baseline after 6 months. In the ADA group, however, levels remained lower than baseline throughout the treatment period. In conclusion, initially, plasma sCD206 in early RA patients decreased in accordance with disease activity and initiation of DMARD treatment. Treatment with anti-TNFα preserved......Rheumatoid arthritis (RA) is characterized by chronic joint inflammation and infiltration by activated macrophages. TNFα is a central mediator in this process. The mannose receptor, CD206, is a scavenger receptor expressed by M2A-macrophages and dendritic cells. It is involved in collagen...

  15. Physicochemical properties of sugar palm starch film: Effect of concentration and plasticizer type

    Science.gov (United States)

    Prasetyo, D. J.; Apriyana, W.; Jatmiko, T. H.; Hernawan; Hayati, S. N.; Rosyida, V. T.; Pranoto, Y.; Poeloengasih, C. D.

    2017-07-01

    In order to find the best formula for capsule shell production, this present work dealt with exploring physicochemical properties of sugar palm (Arenga pinnata) starch film as a function of different kinds and various concentrations of plasticizers. The films were prepared by casting method at different formula: starch 9-11%, glycerol or sorbitol 35-45% and polyethylene-glycol 400 (PEG 400) 5-9%. Appearance, thickness, retraction ratio, moisture content, swelling behavior and solubility of the film in water were analyzed. Both glycerol and sorbitol are compatible with starch matrix. On the contrary, PEG 400 did not form a film with suitable characteristics. The result reveals that glycerol- and sorbitol-plasticized films appeared translucent, homogenous, smooth and slightly brown in all formulas. Different type and concentration of plasticizers altered the physicochemical of film in different ways. The sorbitol-plasticized film had lower moisture content (≤ 10%) than that of glycerol-plasticized film (≥ 18%). In contrast, film plasticized with sorbitol showed higher solubility in water (28-35%) than glycerol-plasticized film (22-28%). As the concentration of both plasticizers increased, there was an increasing tendency on thickness and solubility in water. Conversely, retraction ratio and swelling degree decreased when both plasticizers concentration increased. In conclusion, the sorbitol-plasticized film showed a potency to be developed as hard capsule material.

  16. PROCESS OF OBTAINING OF SUGAR FROM SUGAR BEET AND INFLUENCE ON ITS QUALITY

    Directory of Open Access Journals (Sweden)

    Marián Tokár

    2011-02-01

    Full Text Available Cooking of massecuites has been study in the connection with different particle size distribution of white sugar. During the crystallization is possible to operate with parameters which have influence on particle size of white sugar. Dry matter of juice in crystallizer, volume of the standard syrup in crystallizer and heating curve of crystallization process are constant parameters in this process. Quantity of slurry (seed magma crystallizate and volume of slurry massecuite are parameters which can be changed for control the particle size distribution of white sugar. Five variants of viable parameters have been trying for obtain ideal particle size distribution of white sugar. As a best has been evaluated variant with 1100 cm3 of slurry and 20 % of volume of slurry massecuite in crystallizer. This variant has had the crystals proportions captured by the sieves between 1.00 and 0.40 mm with minimal differences in weight. More results have been related to reduction of losses of sugar in molasses with the right setting for the line of cooling crystallization process. The looses of sugar can be reduced by adding two coolers in the end of cooling crystalization process what will decrease a temperature to 40 ° C. This temperature will lead to more efficient crystallization in the cooling crystallization process.doi:10.5219/122

  17. USE CELLULOSE FOR CLEANING CONCENTRATED SUGAR SOLUTIONS

    Directory of Open Access Journals (Sweden)

    N. G. Kul’neva

    2015-01-01

    Full Text Available Summary. Producing high quality intermediate products in the boiling-crystallization station is an actual problem of sugar production. In the production of white sugar brown sugar syrup is not further purified that decreases the quality of the end product. Studies have been conducted using cellulose as an adsorbent for the purification of concentrated sugar solutions, having affinity to dyes and other impurities. Research have been carried out with the intermediate products of the Lebedyan sugar plant. Test results have shown cellulose ability to adsorb the dyes in sugar production. The influence of the adsorbent concentration and the mass fraction of solids in the syrup on the decolorization effect has been studied; rational process parameters have been obtained. It has been found that proceeding an additional adsorption purification of brown sugars syrup allows to reduce the solution color, increase the amount and quality of the end product. Adsorbing means, received from production wastes on the basis of organic resources, have many advantages: economical, environmentally friendly for disposal, safe to use, reliable and efficient in use. Conducted research on using cellulose as adsorbent for treatment of concentrated sugar solutions, having an affinity for colouring matter and other impurities. The experiments were carried out on the intermediates Lebedyanskiy sugar factory. The test results showed the ability of cellulose to adsorb coloring matter of sugar production. To evaluate the effect of bleaching depending on the mass fraction of dry substances prepared yellow juice filtration of sugar concentration of 55, 60, 65 % with subsequent adsorption purification of cellulose. The results of the experiment built adsorption isotherm of dyestuffs. The influence of the concentration of the adsorbent and a mass fraction of solids of juice filtration on the efficiency of decolorization obtained by rational parameters of the process. It is

  18. A decreased soluble Klotho level with normal eGFR, FGF23, serum phosphate, and FEP in an ADPKD patient with enlarged kidneys due to multiple cysts.

    Science.gov (United States)

    Kanai, Takahiro; Shiizaki, Kazuhiro; Betsui, Hiroyuki; Aoyagi, Jun; Yamagata, Takanori

    2018-05-16

    Autosomal dominant polycystic kidney disease (ADPKD) is the most common hereditary renal disorder. ADPKD is characterized clinically by the presence of multiple bilateral renal cysts that lead to chronic renal failure. The cysts evolve from renal tubular epithelial cells that express the Klotho gene. Notably, Klotho acts as a co-receptor for fibroblast growth factor 23 (FGF23); in this context, it induces phosphaturia and maintains serum phosphate at a normal level. Many reports have shown that decreases in the soluble Klotho level and increases in the FGF23 level are associated with glomerular filtration rate (GFR) decline, but a recent study observed these changes in patient with normal eGFR. It remains unclear whether the decrease in the Klotho level precedes the increase in FGF23. Here, we present an ADPKD patient with enlarged kidneys due to multiple cysts who had a decreased soluble Klotho level but a normal eGFR and a normal FGF23 level. The patient's serum phosphate level was normal, as was the fractional excretion of phosphate (FEP). This appears to be the first reported case to show a decreased soluble Klotho level plus normal eGFR, FGF23, and FEP. These results suggest that Klotho decreases before FGF23 increases and further suggest that Klotho is not required to maintain normal serum phosphate levels in ADPKD if the FEP and serum phosphate levels are normal.

  19. Free sugar contents in onion bulbs on different cultivars and different production areas, and their changes by storage and gamma-irradiation. Free sugars in onion bulbs, 1

    Energy Technology Data Exchange (ETDEWEB)

    Nishibori, Sukie [Tokaigakuen Women' s Coll., Nagoya (Japan); Namiki, Kazuko

    1982-05-01

    Changes in the contents of free sugar in onion bulbs of different cultivars and different production areas were investigated during storage and processing by using gas-chromatography. No significant difference was observed in the total free sugar contents among the onions of different cultivars and different production areas except early season's cultivars which showed a slightly lower content of sugar. The free sugar was mainly composed with glucose (1.7 - 3.2%), fructose (1.1 - 2.5%) and sucrose (0.7 - 2.6%), and the composition was somewhat different among the onions of different cultivars and different production areas. During the storage at 4 - 5/sup 0/C, the total free suger contents gradually decreased during the first few months but more markedly to about 30% after sprouting. The middle part of onion bulbs contained more total free sugar than the other parts (middle part, 5.6 ..-->.. 3.5%, inner part, 5.2 ..-->.. 3.2%, outer part, 4.8 ..-->.. 1.5%), and the total free sugar contents in the outer part decreased markedly during the storage. The changes of the sugar contents after the freeze-drying were almost negligible, but showed significant decrease after the hot-air drying, especially for sucrose. The gamma irradiation with 5 - 50 krad gave no appreciable effects on the total free sugar contents though it seemed to make a slight increase in the sucrose content.

  20. The influence of the extraction parameters on the quality of dried sugar beet pulp

    Directory of Open Access Journals (Sweden)

    Bogdanović Branislav V.

    2013-01-01

    Full Text Available Growing demand for better quality and cheaper products put on the need to industry to balance between the two kinds of needs, which are the reduction of production costs and to ensure a high quality product. This is the case with dried sugar beet pulp as a byproduct of sugar refining process. One of the possibilities to reduce energy consumption is savings in the process of thermal dehydration in the process of the conventional drying of pulp. Pulp drying is one third of total energy consumption in the sugar factory. Acidification of water extraction result in pulp that could be pressed better and in other hand in the juice with less non-sucrose compounds. Keeping the extraction process under certain conditions, directly affects on the quality of the extracted pulp and on effects of their further processing. This paper examines the impact of the extraction parameters of sugar from sugar beet and agents of acidification for water extraction (sulphurous acid, hydrogen peroxide on the quality of dried sugar beet pulp. Extraction of sugar from sugar beet cossettes by hydrogen peroxide at pH 5.5 to 8.5 does not cause changes in mechanical properties of sugar beet cossettes and in water retention coefficient, but increases the brightness of dried sugar beet pulp for about 5-7 units, which solubility in water does not depend on the applied concentration of extracting agents, but increases in dependence on the duration of the extraction. Sugar beet pulp extracted at pH 11.0 has reduced strength, they are highly hydrated, and under the force they lose their shape, binding a larger amount of water thus reducing the effect of their pressing. The changes in microstructure were probably accompanied by segmenting the linear parts of macromolecules and are reflected in the increase of water retention capacity and increase the solubility of dried sugar beet pulp for two to three times. Under the same conditions of extraction, sulphurous acid produces similar

  1. COMPUTER SYSTEM FOR DETERMINATION OF COST DAILY SUGAR PRODUCTION AND INCIDENTS DECISIONS FOR COMPANIES SUGAR (SACODI

    Directory of Open Access Journals (Sweden)

    Alejandro Álvarez-Navarro

    2016-01-01

    Full Text Available The process of sugar production is complex; anything that affects this chain has direct repercussions in the sugar production’s costs, it’s synthetic and decisive indicator for the taking of decisions. Currently the Cuban sugar factory determine this cost weekly, for that, its process of taking of decisions is affected. Looking for solutions to this problem, the present work, being part of a territorial project approved by CITMA, intended to calculate the cost of production daily, weekly, monthly and accumulated until indicated date, according to an adaptation to the methodology used by the National Costs System of sugarcane created by the MINAZ, it’s supported by a computer system denominated SACODI. This adaptation registers the physical and economic indicators of all direct and indirect expenses of the  sugarcane and besides this information generates an economic-mathematical model of goal programming whose solution indicates the best balance in amount of sugar of the entities of the sugar factory, in short term. The implementation of the system in the sugar factory «Julio A. Mella» in Santiago de Cuba in the sugar-cane production 08-09 produced an estimate of decrease of the cost of until 3,5 % for the taking of better decisions. 

  2. Grading sugar pine saw logs in trees.

    Science.gov (United States)

    John W. Henley

    1972-01-01

    Small limbs and small overgrown limbs cause problems when grading saw logs in sugar pine trees. Surface characteristics and lumber recovery information for 426 logs from 64 sugar pine trees were examined. Resulting modifications in the grading specification that allow a grader to ignore small limbs and small limb indicators do not appear to decrease the performance of...

  3. Hydrocolloids Decrease the Digestibility of Corn Starch, Soy Protein, and Skim Milk and the Antioxidant Capacity of Grape Juice.

    Science.gov (United States)

    Yi, Yue; Jeon, Hyeong-Ju; Yoon, Sun; Lee, Seung-Min

    2015-12-01

    Hydrocolloids have many applications in foods including their use in dysphagia diets. We aimed to evaluate whether hydrocolloids in foods affect the digestibility of starch and protein, and their effects on antioxidant capacity. The thickening hydrocolloids: locust bean gum and carboxymethyl cellulose, and the gel-forming agents: agar agar, konjac-glucomannan, and Hot & Soft Plus were blended with corn starch and soy protein, skim milk, or grape juice and were examined for their in vitro-digestability by comparing the reducing sugar and trichloroacetic acid (TCA)-soluble peptide, for antioxidant capacity by total polyphenol contents and the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity. The hydrocolloids resulted in a decrease in starch digestibility with the gel-forming agents. Hydrocolloids diminished TCA-soluble peptides in skim milk compared to soy protein with the exception of locust bean gum and decreased free radical scavenging capacities and total phenolic contents in grape juice. Our findings may provide evidence for the use of hydro-colloids for people at risk of nutritional deficiencies such as dysphagia patients.

  4. Blending of soluble corn fiber with pullulan, sorbitol, or fructose attenuates glycemic and insulinemic responses in the dog and affects hydrolytic digestion in vitro.

    Science.gov (United States)

    de Godoy, M R C; Knapp, B K; Bauer, L L; Swanson, K S; Fahey, G C

    2013-08-01

    The objective of these experiments was to measure in vitro hydrolytic digestion and glycemic and insulinemic responses of select carbohydrate blends, all containing the novel carbohydrate soluble corn fiber (SCF). Two SCF that varied in their method of production were used to formulate the carbohydrate blends. One set of blends contained a SCF that was spray dried (SCFsd) and then blended with different amounts of either pullulan, sorbitol, or fructose. The other set of blends contained a SCF produced using longer evaporation time (SCF) and then blended with different ratios of pullulan, sorbitol, and fructose. Free sugar concentrations found in the individual SCFsd and SCF substrates were low but varied. Spray-dried soluble corn fiber had a reduced free sugar concentration compared with SCF (2.8 vs. 14.2%). Glucose was the main free sugar found in both SCFsd and SCF but at different concentrations (2.7 vs. 12.7%, respectively). The majority of the SCFsd blends were completely hydrolyzed to their monosaccharide components. Glucose accounted for most of the hydrolyzed monosaccharides for SCFsd and all the SCFsd blends. Hydrolyzed monosaccharide concentrations for the SCF:pullulan:sorbitol:fructose blends followed similar trends to the SCFsd blends where greater percentages of fructose and sorbitol resulted in decreased (P sorbitol. Total released monosaccharides were high in SCFsd blends containing either 50% fructose or sorbitol, but the combination resulted in reduced concentrations of glucose released (P sorbitol:fructose blends also had intermediate to high released monosaccharides as a result of in vitro hydrolytic digestion. All SCF blends resulted in decreased glycemic and insulinemic responses compared with the maltodextrin control (P sorbitol in the blends had the greatest impact on glycemic and insulinemic responses, even at concentrations as low as 5% of the blends. Overall, SCF and their blends may prove beneficial as components of low glycemic

  5. RESEARCH OF LIMY AND CARBONATE SYSTEM OF SUGAR PRODUCTION

    Directory of Open Access Journals (Sweden)

    N. G. Kulneva

    2012-01-01

    Full Text Available Influence of рН and temperature on activity of suspension of lime and carbonate in sugar production is investigated. Possibility of decrease in a consumption of reagents on purification of production sugar solutions is established.

  6. Solubility of Carbon in Nanocrystalline -Iron

    OpenAIRE

    Alexander Kirchner; Bernd Kieback

    2012-01-01

    A thermodynamic model for nanocrystalline interstitial alloys is presented. The equilibrium solid solubility of carbon in -iron is calculated for given grain size. Inside the strained nanograins local variation of the carbon content is predicted. Due to the nonlinear relation between strain and solubility, the averaged solubility in the grain interior increases with decreasing grain size. The majority of the global solubility enhancement is due to grain boundary enrichment however. Therefor...

  7. Cut Back on Your Kid's Sweet Treats: 10 Tips to Decrease Added Sugars

    Science.gov (United States)

    United States Department of Agriculture 10 tips Nutrition Education Series MyPlate MyWins Based on the Dietary Guidelines for Americans Cut back on your kid’s sweet treats Set your kids on a path for lifelong healthy eating by limiting the amount of added sugars they ...

  8. Association of Dietary Sugars and Sugar-Sweetened Beverage Intake with Obesity in Korean Children and Adolescents

    Directory of Open Access Journals (Sweden)

    Kyungho Ha

    2016-01-01

    Full Text Available Few studies have examined the association between dietary sugar intake and obesity in Asian children and adolescents. We evaluated the association of dietary sugar intake and its food source with obesity in Korean children and adolescents. In this cross-sectional analysis, data were obtained from five studies conducted between 2002 and 2011. The study included 2599 children and adolescents who had completed more than three days of dietary records and had anthropometric data. Total sugar intake was higher in girls than in boys (54.3 g for girls and 46.6 g for boys, p < 0.0001. Sugar intake from milk and fruits was inversely associated with overweight or obesity in girls only (OR for overweight, 0.52; 95% CI, 0.32–0.84; p for trend = 0.0246 and OR for obesity, 0.42; 95% CI, 0.23–0.79; p for trend = 0.0113. Sugar-sweetened beverage (SSB consumption was not associated with obesity in girls, while boys had lower odds ratios for obesity (OR for obesity, 0.52; 95% CI, 0.26–1.05; p for trend = 0.0310. These results suggest that total sugars and SSB intake in Asian children and adolescents remains relatively low and sugar intake from milk and fruits is associated with a decreased risk of overweight or obesity, especially in girls.

  9. Association of Dietary Sugars and Sugar-Sweetened Beverage Intake with Obesity in Korean Children and Adolescents.

    Science.gov (United States)

    Ha, Kyungho; Chung, Sangwon; Lee, Haeng-Shin; Kim, Cho-il; Joung, Hyojee; Paik, Hee-Young; Song, YoonJu

    2016-01-08

    Few studies have examined the association between dietary sugar intake and obesity in Asian children and adolescents. We evaluated the association of dietary sugar intake and its food source with obesity in Korean children and adolescents. In this cross-sectional analysis, data were obtained from five studies conducted between 2002 and 2011. The study included 2599 children and adolescents who had completed more than three days of dietary records and had anthropometric data. Total sugar intake was higher in girls than in boys (54.3 g for girls and 46.6 g for boys, p < 0.0001). Sugar intake from milk and fruits was inversely associated with overweight or obesity in girls only (OR for overweight, 0.52; 95% CI, 0.32-0.84; p for trend = 0.0246 and OR for obesity, 0.42; 95% CI, 0.23-0.79; p for trend = 0.0113). Sugar-sweetened beverage (SSB) consumption was not associated with obesity in girls, while boys had lower odds ratios for obesity (OR for obesity, 0.52; 95% CI, 0.26-1.05; p for trend = 0.0310). These results suggest that total sugars and SSB intake in Asian children and adolescents remains relatively low and sugar intake from milk and fruits is associated with a decreased risk of overweight or obesity, especially in girls.

  10. NMR-Based Metabolic Profiling of Field-Grown Leaves from Sugar Beet Plants Harbouring Different Levels of Resistance to Cercospora Leaf Spot Disease

    Directory of Open Access Journals (Sweden)

    Yasuyo Sekiyama

    2017-01-01

    Full Text Available Cercospora leaf spot (CLS is one of the most serious leaf diseases for sugar beet (Beta vulgaris L. worldwide. The breeding of sugar beet cultivars with both high CLS resistance and high yield is a major challenge for breeders. In this study, we report the nuclear magnetic resonance (NMR-based metabolic profiling of field-grown leaves for a subset of sugar beet genotypes harbouring different levels of CLS resistance. Leaves were collected from 12 sugar beet genotypes at four time points: seedling, early growth, root enlargement, and disease development stages. 1H-NMR spectra of foliar metabolites soluble in a deuterium-oxide (D2O-based buffer were acquired and subjected to multivariate analyses. A principal component analysis (PCA of the NMR data from the sugar beet leaves shows clear differences among the growth stages. At the later time points, the sugar and glycine betaine contents were increased, whereas the choline content was decreased. The relationship between the foliar metabolite profiles and resistance level to CLS was examined by combining partial least squares projection to latent structure (PLS or orthogonal PLS (OPLS analysis and univariate analyses. It was difficult to build a robust model for predicting precisely the disease severity indices (DSIs of each genotype; however, GABA and Gln differentiated susceptible genotypes (genotypes with weak resistance from resistant genotypes (genotypes with resistance greater than a moderate level before inoculation tests. The results suggested that breeders might exclude susceptible genotypes from breeding programs based on foliar metabolites profiled without inoculation tests, which require an enormous amount of time and effort.

  11. Sugar Price Supports and Taxation

    Science.gov (United States)

    Dilk, Abby; Savaiano, Dennis A.

    2017-01-01

    Domestic US sugar production has been protected by government policy for the past 82 years, resulting in elevated domestic prices and an estimated annual (2013) $1.4 billion dollar “tax” on consumers. These elevated prices and the simultaneous federal support for domestic corn production have ensured a strong market for high-fructose corn syrup. Americans have dramatically increased their consumption of caloric sweeteners during the same period. Consumption of “empty” calories (ie, foods with low-nutrient/high-caloric density)—sugar and high-fructose corn syrup being the primary sources—is considered by most public health experts to be a key contributing factor to the rise in obesity. There have been substantial efforts to tax sugar-sweetened beverages (SSBs) to both reduce consumption and provide a source of funds for nutrition education, thereby emulating the tobacco tax model. Volume-based SSB taxes levy the tax rate per ounce of liquid, where some are only imposed on beverages with added sugar content exceeding a set threshold. Nonetheless, volume-based taxes have significant limitations in encouraging consumers to reduce their caloric intake due to a lack of transparency at the point of purchase. Thus, it is hypothesized that point-of-purchase, nutrient-specific excise taxes on SSBs would be more effective at reducing sugar consumption. However, all SSB taxes are limited by the possibility that consumers may compensate their decreased intake from SSBs with other high-calorie junk foods. Furthermore, there are no existing studies to provide evidence on how SSB taxes will impact obesity rates in the long term. The paradox of sugar prices is that Americans have paid higher prices for sugar to protect domestic production for more than 80 years, and now, Americans are being asked to pay even more to promote public health. The effective use of sugar taxes should be considered based on their merits in reducing sugar consumption and making available a new

  12. Characterization of soluble dietary fiber from Moringa oleifera seeds and its immunomodulatory effects.

    Science.gov (United States)

    Anudeep, Sandanamudi; Prasanna, Vaddi K; Adya, Shruthi M; Radha, Cheruppanpullil

    2016-10-01

    Moringa oleifera (moringa or drumstick) seeds are a potential source of dietary fiber with 6.5% w/w soluble dietary fiber. Biochemical characterization of moringa seed soluble fiber revealed that it is a glycoprotein with 5% neutral sugars. Arabinose and xylose are the major neutral sugars identified by gas liquid chromatography (GLC). Moringa seed soluble fiber was identified as protease resistant-glycoprotein and termed as moringa seed resistant protein (MSRP). MSRP was found to be a homodimer (18kDa) containing two 9kDa monomeric units as revealed by SDS-PAGE analysis with pI 10.8. Immunostimulating activity of MSRP was assessed by murine splenocyte proliferation and production of NO from macrophages. MSRP at low concentration (0.01μg/well) strongly increased proliferation of splenocytes, while MSRP at high concentration weakly responded. MSRP induced 6-fold increase in NO production when compared to the control which indicates the activation of macrophages. MSRP isolated from defatted moringa seed flour is a potent mitogen, enhancing the proliferation of lymphocytes and inducing NO from macrophages. This study concludes that moringa seed is a potential nutritional source to promote the immune system of the host. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. Elevated atmospheric CO2 affected photosynthetic products in wheat seedlings and biological activity in rhizosphere soil under cadmium stress.

    Science.gov (United States)

    Jia, Xia; Liu, Tuo; Zhao, Yonghua; He, Yunhua; Yang, Mingyan

    2016-01-01

    The objective of this study was to investigate the effects of elevated CO2 (700 ± 23 μmol mol(-1)) on photosynthetic products in wheat seedlings and on organic compounds and biological activity in rhizosphere soil under cadmium (Cd) stress. Elevated CO2 was associated with decreased quantities of reducing sugars, starch, and soluble amino acids, and with increased quantities of soluble sugars, total sugars, and soluble proteins in wheat seedlings under Cd stress. The contents of total soluble sugars, total free amino acids, total soluble phenolic acids, and total organic acids in the rhizosphere soil under Cd stress were improved by elevated CO2. Compared to Cd stress alone, the activity of amylase, phenol oxidase, urease, L-asparaginase, β-glucosidase, neutral phosphatase, and fluorescein diacetate increased under elevated CO2 in combination with Cd stress; only cellulase activity decreased. Bacterial abundance in rhizosphere soil was stimulated by elevated CO2 at low Cd concentrations (1.31-5.31 mg Cd kg(-1) dry soil). Actinomycetes, total microbial abundance, and fungi decreased under the combined conditions at 5.31-10.31 mg Cd kg(-1) dry soil. In conclusion, increased production of soluble sugars, total sugars, and proteins in wheat seedlings under elevated CO2 + Cd stress led to greater quantities of organic compounds in the rhizosphere soil relative to seedlings grown under Cd stress only. Elevated CO2 concentrations could moderate the effects of heavy metal pollution on enzyme activity and microorganism abundance in rhizosphere soils, thus improving soil fertility and the microecological rhizosphere environment of wheat under Cd stress.

  14. Studies on the sugars development of irradiated potatoes receiving different nitrogen levels during growth and stored at different temperatures

    International Nuclear Information System (INIS)

    Badshah, N.; Iritani, W.M.; Rom, C.R.; Patterson, M.E.

    1990-01-01

    Tubers of Russet Burbank potatoes from 0, 181.8 and 363.6 kg/ha nitrogen were irradiated with 0, 0.05, 0.1 and 0.2 kGy of gamma rays (Co 60 source) and stored for three months at temperatures of 10 and 15.5°C. Changes in reducing sugars and sucrose contents were significantly influenced by nitrogen and irradiation levels while storage temperatures had no significant effect. Nitrogen and irradiation significantly decreased reducing and non-reducing sugars while temperature had no significant effect. Reducing sugars decreased with increasing levels of nitrogen and irradiation. Tubers from zero fertilizer regime developed 1.5% reducing sugars. Irradiation at 0.2 kGy dosage decreased reducing sugars from 1.7 to 0.9%. The breakdown of non-reducing sugars increased with increasing nitrogen levels but decreased with irradiation. Tubers from the maximum nitrogen plot had a 36% decrease of non-reducing sugars. Irradiation at 0.1 kGy dosage had the least change (4.9% decrease) of non-reducing sugars. (author)

  15. Soluble Fermentable Dietary Fibre (Pectin) Decreases Caloric Intake, Adiposity and Lipidaemia in High-Fat Diet-Induced Obese Rats

    Science.gov (United States)

    Adam, Clare L.; Thomson, Lynn M.; Williams, Patricia A.; Ross, Alexander W.

    2015-01-01

    Consumption of a high fat diet promotes obesity and poor metabolic health, both of which may be improved by decreasing caloric intake. Satiety-inducing ingredients such as dietary fibre may be beneficial and this study investigates in diet-induced obese (DIO) rats the effects of high or low fat diet with or without soluble fermentable fibre (pectin). In two independently replicated experiments, young adult male DIO rats that had been reared on high fat diet (HF; 45% energy from fat) were given HF, low fat diet (LF; 10% energy from fat), HF with 10% w/w pectin (HF+P), or LF with 10% w/w pectin (LF+P) ad libitum for 4 weeks (n = 8/group/experiment). Food intake, body weight, body composition (by magnetic resonance imaging), plasma hormones, and plasma and liver lipid concentrations were measured. Caloric intake and body weight gain were greatest in HF, lower in LF and HF+P, and lowest in the LF+P group. Body fat mass increased in HF, was maintained in LF, but decreased significantly in LF+P and HF+P groups. Final plasma leptin, insulin, total cholesterol and triglycerides were lower, and plasma satiety hormone PYY concentrations were higher, in LF+P and HF+P than in LF and HF groups, respectively. Total fat and triglyceride concentrations in liver were greatest in HF, lower in LF and HF+P, and lowest in the LF+P group. Therefore, the inclusion of soluble fibre in a high fat (or low fat) diet promoted increased satiety and decreased caloric intake, weight gain, adiposity, lipidaemia, leptinaemia and insulinaemia. These data support the potential of fermentable dietary fibre for weight loss and improving metabolic health in obesity. PMID:26447990

  16. Co-Production of Fungal Biomass Derived Constituents and Ethanol from Citrus Wastes Free Sugars without Auxiliary Nutrients in Airlift Bioreactor.

    Science.gov (United States)

    Satari, Behzad; Karimi, Keikhosro; Taherzadeh, Mohammad J; Zamani, Akram

    2016-02-26

    The potential of two zygomycetes fungi, Mucor indicus and Rhizopus oryzae, in assimilating citrus waste free sugars (CWFS) and producing fungal chitosan, oil, and protein as well as ethanol was investigated. Extraction of free sugars from citrus waste can reduce its environmental impact by decreasing the possibility of wild microorganisms growth and formation of bad odors, a typical problem facing the citrus industries. A total sugar concentration of 25.1 g/L was obtained by water extraction of citrus waste at room temperature, used for fungal cultivation in shake flasks and airlift bioreactor with no additional nutrients. In shake flasks cultivations, the fungi were only able to assimilate glucose, while fructose remained almost intact. In contrast, the cultivation of M. indicus and R. oryzae in the four-liter airlift bioreactor resulted in the consumption of almost all sugars and production of 250 and 280 g fungal biomass per kg of consumed sugar, respectively. These biomasses correspondingly contained 40% and 51% protein and 9.8% and 4.4% oil. Furthermore, the fungal cell walls, obtained after removing the alkali soluble fraction of the fungi, contained 0.61 and 0.69 g chitin and chitosan per g of cell wall for M. indicus and R. oryzae, respectively. Moreover, the maximum ethanol yield of 36% and 18% was obtained from M. indicus and R. oryzae, respectively. Furthermore, that M. indicus grew as clump mycelia in the airlift bioreactor, while R. oryzae formed spherical suspended pellets, is a promising feature towards industrialization of the process.

  17. Co-Production of Fungal Biomass Derived Constituents and Ethanol from Citrus Wastes Free Sugars without Auxiliary Nutrients in Airlift Bioreactor

    Directory of Open Access Journals (Sweden)

    Behzad Satari

    2016-02-01

    Full Text Available The potential of two zygomycetes fungi, Mucor indicus and Rhizopus oryzae, in assimilating citrus waste free sugars (CWFS and producing fungal chitosan, oil, and protein as well as ethanol was investigated. Extraction of free sugars from citrus waste can reduce its environmental impact by decreasing the possibility of wild microorganisms growth and formation of bad odors, a typical problem facing the citrus industries. A total sugar concentration of 25.1 g/L was obtained by water extraction of citrus waste at room temperature, used for fungal cultivation in shake flasks and airlift bioreactor with no additional nutrients. In shake flasks cultivations, the fungi were only able to assimilate glucose, while fructose remained almost intact. In contrast, the cultivation of M. indicus and R. oryzae in the four-liter airlift bioreactor resulted in the consumption of almost all sugars and production of 250 and 280 g fungal biomass per kg of consumed sugar, respectively. These biomasses correspondingly contained 40% and 51% protein and 9.8% and 4.4% oil. Furthermore, the fungal cell walls, obtained after removing the alkali soluble fraction of the fungi, contained 0.61 and 0.69 g chitin and chitosan per g of cell wall for M. indicus and R. oryzae, respectively. Moreover, the maximum ethanol yield of 36% and 18% was obtained from M. indicus and R. oryzae, respectively. Furthermore, that M. indicus grew as clump mycelia in the airlift bioreactor, while R. oryzae formed spherical suspended pellets, is a promising feature towards industrialization of the process.

  18. Formation of Polyphenol-Denatured Protein Flocs in Alcohol Beverages Sweetened with Refined Cane Sugars.

    Science.gov (United States)

    Eggleston, Gillian; Triplett, Alexa

    2017-11-08

    The sporadic appearance of floc from refined, white cane sugars in alcohol beverages remains a technical problem for both beverage manufacturers and sugar refiners. Cane invert sugars mixed with 60% pure alcohol and water increased light scattering by up to ∼1000-fold. Insoluble and soluble starch, fat, inorganic ash, oligosaccharides, Brix, and pH were not involved in the prevailing floc-formation mechanism. Strong polynomial correlations existed between the haze floc and indicator values (IVs) (color at 420 nm pH 9.0/color at pH 4.0-an indirect measure of polyphenolic and flavonoid colorants) (R 2 = 0.815) and protein (R 2 = 0.819) content of the invert sugars. Ethanol-induced denaturation of the protein exposed hydrophobic polyphenol-binding sites that were further exposed when heated to 80 °C. A tentative mechanism for floc formation was advanced by molecular probing with a haze (floc) active protein and polyphenol as well as polar, nonpolar, and ionic solvents.

  19. Ethanol from sugar cane bagasse. Contribution to atmospheric CO[sub 2] decrease. El etanol de bagazo como combustible. Contribucion a la reduccion del CO[sub 2] atmosferico

    Energy Technology Data Exchange (ETDEWEB)

    Cardenas, G.J. (Estacion Experimental Agroindustrial Obispo Colombres. Tucuman (Argentina))

    1993-03-01

    The current problem related to the increasing concentration of atmospheric CO[sub 2] produced by the industrial use of fossil fuels is reviewed. An analysis of the contribution that the use of ethanol from sugar cane bagasse might have on CO[sub 2] decrease is described. (Author)

  20. Taxation and Sugar-Sweetened Beverages: Position of Dietitians of Canada.

    Science.gov (United States)

    2016-06-01

    Dietitians of Canada recommends that an excise tax of at least 10-20% be applied to sugar-sweetened beverages sold in Canada given the negative impact of these products on the health of the population and the viability of taxation as a means to reduce consumption. For the greatest impact, taxation measures should be combined with other policy interventions such as increasing access to healthy foods while decreasing access to unhealthy foods in schools, daycares, and recreation facilities; restrictions on the marketing of foods and beverages to children; and effective, long-term educational initiatives. This position is based on a comprehensive review of the literature. The Canadian population is experiencing high rates of obesity and excess weight. There is moderate quality evidence linking consumption of sugar-sweetened beverages to excess weight, obesity, and chronic disease onset in children and adults. Taxation of sugar-sweetened beverages holds substantiated potential for decreasing its consumption. Based on economic models and results from recent taxation efforts, an excise tax can lead to a decline in sugar-sweetened beverage purchase and consumption. Taxation of up to 20% can lead to a consumption decrease by approximately 10% in the first year of its implementation, with a postulated 2.6% decrease in weight per person on average. Revenue generated from taxation can be used to fund other obesity reduction initiatives. A number of influential national organizations support a tax on sugar-sweetened beverages.

  1. Liming Poultry Manures to Kill Pathogens and Decrease Soluble Phosphorus

    International Nuclear Information System (INIS)

    Maguire, R.; Hesterberg, D.; Gernat, A.; Anderson, K.; Wineland, M.; Grimes, J.

    2006-01-01

    Received for publication September 9, 2005. Stabilizing phosphorus (P) in poultry waste to reduce P losses from manured soils is important to protect surface waters, while pathogens in manures are an emerging issue. This study was conducted to evaluate CaO and Ca(OH) 2 for killing manure bacterial populations (pathogens) and stabilizing P in poultry wastes and to investigate the influence on soils following amendment with the treated wastes. Layer manure and broiler litter varying in moisture content were treated with CaO and Ca(OH) 2 at rates of 2.5, 5, 10, and 15% by weight. All treated wastes were analyzed for microbial plate counts, pH, and water-soluble phosphorus (WSP), while a few selected layer manures were analyzed by phosphorus X-ray absorption near edge structure (XANES). A loamy sand and a silt loam were amended with broiler litter and layer manure treated with CaO at rates of 0, 2.5, 5, 10, and 15% and soil WSP and pH were measured at times 1, 8, and 29 d. Liming reduced bacterial populations, with greater rates of lime leading to greater reductions; for example 10% CaO applied to 20% solids broiler litter reduced the plate counts from 793 000 to 6500 mL -1 . Liming also reduced the WSP in the manures by over 90% in all cases where at least 10% CaO was added. Liming the manures also reduced WSP in soils immediately following application and raised soil pH. The liming process used successfully reduced plate counts and concerns about P losses in runoff following land application of these limed products due to decreased WSP

  2. Sweet taste in apple: the role of sorbitol, individual sugars, organic acids and volatile compounds

    Science.gov (United States)

    Aprea, Eugenio; Charles, Mathilde; Endrizzi, Isabella; Laura Corollaro, Maria; Betta, Emanuela; Biasioli, Franco; Gasperi, Flavia

    2017-03-01

    Sweetness is one of the main drivers of consumer preference, and thus is given high priority in apple breeding programmes. Due to the complexity of sweetness evaluation, soluble solid content (SSC) is commonly used as an estimation of this trait. Nevertheless, it has been demonstrated that SSC and sweet taste are poorly correlated. Though individual sugar content may vary greatly between and within apple cultivars, no previous study has tried to investigate the relationship between the amount of individual sugars, or ratios of these, and apple sweetness. In this work, we quantified the major sugars (sucrose, glucose, fructose, xylose) and sorbitol and explored their influence on perceived sweetness in apple; we also related this to malic acid content, SSC and volatile compounds. Our data confirmed that the correlation between sweetness and SSC is weak. We found that sorbitol content correlates (similarly to SSC) with perceived sweetness better than any other single sugar or total sugar content. The single sugars show no differentiable importance in determining apple sweetness. Our predictive model based on partial least squares regression shows that after sorbitol and SSC, the most important contribution to apple sweetness is provided by several volatile compounds, mainly esters and farnesene.

  3. Cloning, localization and expression analysis of vacuolar sugar transporters in the CAM plant Ananas comosus (pineapple).

    Science.gov (United States)

    Antony, Edna; Taybi, Tahar; Courbot, Mikaël; Mugford, Sam T; Smith, J Andrew C; Borland, Anne M

    2008-01-01

    In photosynthetic tissues of the CAM plant pineapple (Ananas comosus), storage of soluble sugars in the central vacuole during the daytime and their remobilization at night is required to provide carbon skeletons for nocturnal CO(2) fixation. However, soluble sugars produced photosynthetically must also be exported to support growth processes in heterotrophic tissues. To begin to address how vacuolar sugar storage and assimilate partitioning are regulated in A. comosus, degenerate PCR and cDNA library screening were used to clone three candidate sugar transporters from the leaves of this species. Subcellular localization of the three transporters was investigated via expression of YFP-fusion proteins in tobacco epidermal cells and their co-localization with subcellular markers by confocal microscopy. Using this strategy, a putative hexose transporter (AcMST1) and a putative inositol transporter (AcINT1) were identified that both localized to the tonoplast, whereas a putative sucrose transporter (AcSUT1) was found to localize to prevacuolar compartments. A cDNA (AcMST2) with high similarity to a recently characterized tonoplast hexose transporter in Arabidopsis was also identified from an A. comosus fruit EST database. Analyses of transcript abundance indicated that AcMST1 was more highly expressed in fruits compared to leaves of A. comosus, whilst transcripts of AcINT1, AcSUT1, and AcMST2 were more abundant in leaves. Transcript abundance of AcINT1, the putative inositol transporter, showed day-night changes comparable to those of other CAM-related transcripts described in Mesembryanthemum crystallinum. The results are discussed in terms of the role of vacuolar sugar transporters in regulating carbon flow during the diel cycle in CAM plants.

  4. Studies on soluble carbohydrates in yellow lupin (Lupinus luteus L. seeds of different age

    Directory of Open Access Journals (Sweden)

    Kazimierz Zalewski

    2011-01-01

    Full Text Available Yellow lupin seeds cv. Juno were stored under laboratory conditions for 2 month, 4, 6 and 8 years. Eighteen soluble carbohydrates were identified in embryonic axes and cotyledons of different age seeds. The concentration of soluble carbohydrates in analysed seeds ranged from 25 to 34% of dry mass. Axes contained more carbohydrates than cotyledons. Stachyose dominated in axes, and verbascose - in cotyledons. Other detected galactosides were: galactinol, galactosyl pinitols and galactosyl chiro-inositols (fagopyritols, but their content was several-fold lower than that of RFOs (in both axes and cotyledons tissues. The concentration of soluble carbohydrates indicated, that sucrose to RFOs mass ratio, or other changes in sugars composition are not indicators of seed storage.

  5. Decreases in high-fat and/or high added sugar food group intake occur when a hypocaloric, low-fat diet is prescribed within a lifestyle intervention: a secondary cohort analysis

    Science.gov (United States)

    Keshani, Vaishali Deepak; Sheikh, Vaishali Keshani; Raynor, Hollie Anne

    2016-01-01

    Background When a hypocaloric, low-fat diet is prescribed, intake of currently consumed foods can decrease, foods naturally low in fat and/or added sugar may increase, or fat- or sugar-modified foods may increase. Objective Examine food group intake change and its relation to reductions in energy and fat intake, and weight during a lifestyle intervention. Design Secondary cohort analysis. Participants One hundred sixty-nine participants (52.0 ± 8.6 years, 34.9 ± 4.5 kg/m2, 92% white, 97.6% non-Hispanic, and 56.8% female) with complete data at 0 and 6 months collected in a research setting. Main Outcome Measures From 3, 24-hr phone dietary recalls, 165 food groups from NDSR software were coded into 25 larger food groups assessing intake of higher fat and/or added sugar food groups vs. naturally lower fat and/or added sugar food groups and into 17 larger food groups assessing intake of non-modified vs. fat- and/or sugar-modified food groups. Statistical Analyses Performed Repeated measures analyses of covariance (intervention group: covariate) assessed changes from 0 to 6 months. Hierarchical regressions examined changes in food group intake and changes in energy intake, percent energy from fat intake, and weight from 0 to 6 months. Results Significant reductions (p hypocaloric, low-fat diet is prescribed, reductions in high-fat and/or high-added sugar food groups occur. Targeting reductions in high-fat meats may improve outcomes. PMID:27436530

  6. Early response of plant cell to carbon deprivation: in vivo 31P-NMR spectroscopy shows a quasi-instantaneous disruption on cytosolic sugars, phosphorylated intermediates of energy metabolism, phosphate partitioning, and intracellular pHs.

    Science.gov (United States)

    Gout, Elisabeth; Bligny, Richard; Douce, Roland; Boisson, Anne-Marie; Rivasseau, Corinne

    2011-01-01

    • In plant cells, sugar starvation triggers a cascade of effects at the scale of 1-2 days. However, very early metabolic response has not yet been investigated. • Soluble phosphorus (P) compounds and intracellular pHs were analysed each 2.5 min intervals in heterotrophic sycamore (Acer pseudoplatanus) cells using in vivo phosphorus nuclear magnetic resonance ((31)P-NMR). • Upon external-sugar withdrawal, the glucose 6-P concentration dropped in the cytosol, but not in plastids. The released inorganic phosphate (Pi) accumulated transiently in the cytosol before influx into the vacuole; nucleotide triphosphate concentration doubled, intracellular pH increased and cell respiration decreased. It was deduced that the cytosolic free-sugar concentration was low, corresponding to only 0.5 mM sucrose in sugar-supplied cells. • The release of sugar from the vacuole and from plastids is insufficient to fully sustain the cell metabolism during starvation, particularly in the very short term. Similarly to Pi-starvation, the cell's first response to sugar starvation occurs in the cytosol and is of a metabolic nature. Unlike the cytoplasm, cytosolic homeostasis is not maintained during starvation. The important metabolic changes following cytosolic sugar exhaustion deliver early endogenous signals that may contribute to trigger rescue metabolism. © The Authors (2010). Journal compilation © New Phytologist Trust (2010).

  7. Decreased concentrations of soluble interleukin-1 receptor accessory protein levels in the peritoneal fluid of women with endometriosis.

    Science.gov (United States)

    Michaud, Nadège; Al-Akoum, Mahéra; Gagnon, Geneviève; Girard, Karine; Blanchet, Pierre; Rousseau, Julie Anne; Akoum, Ali

    2011-12-01

    Interleukin 1 (IL1) may play an important role in endometriosis-associated pelvic inflammation, and natural specific inhibitors, including soluble IL1 receptor accessory protein (sIL1RAcP) and soluble IL1 receptor type 2 (sIL1R2), are critical for counterbalancing the pleiotropic effects of IL1. The objective of this study was to evaluate the levels of sIL1RAcP, together with those of sIL1R2 and IL1β, in the peritoneal fluid of women with and without endometriosis. Peritoneal fluid samples were obtained at laparoscopy and assessed by ELISA. sIL1RAcP concentrations were reduced in endometriosis stages I-II and III-IV. sIL1R2 concentrations were decreased, and those of IL1β were significantly increased in endometriosis stages I-II. sIL1RAcP and sIL1R2 concentrations were significantly decreased in the secretory phase of the menstrual cycle, and IL1β concentrations were elevated in the proliferative and the secretory phases. sIL1RAcP and sIL1R2 concentrations were reduced in women with endometriosis who were infertile, fertile, suffering from pelvic pain or pain-free. However, IL1β concentrations were significantly reduced in women with endometriosis who were infertile or had pelvic pain. These changes may exacerbate the local peritoneal inflammatory reaction observed in women with endometriosis and contribute to endometriosis pathophysiology and the major symptoms of this disease. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  8. Effects of low molecular sugars on the retrogradation of tapioca starch gels during storage.

    Directory of Open Access Journals (Sweden)

    Xiaoyu Zhang

    Full Text Available The effects of low molecular sugars (sucrose, glucose and trehalose on the retrogradation of tapioca starch (TS gels stored at 4°C for different periods were examined with different methods. Decrease in melting enthalpy (ΔHmelt were obtained through differential scanning calorimetry analysis. Analysis of decrease in crystallization rate constant (k and increase in semi-crystallization time (τ1/2 results obtained from retrogradation kinetics indicated that low molecular sugars could retard the retrogradation of TS gels and further revealed trehalose as the best inhibitor among the sugars used in this study. Fourier transform infrared (FTIR analysis indicated that the intensity ratio of 1047 to 1022 cm-1 was increased with the addition of sugars in the order of trehalose > sucrose > glucose. Decrease in hardness parameters and increase in springiness parameters obtained from texture profile analysis (TPA analysis also indicated that low molecular sugars could retard the retrogradation of TS gels. The results of FTIR and TPA showed a consistent sugar effect on starch retrogradation with those of DSC and retrogradation kinetics analysis.

  9. Decreased soluble cell adhesion molecules after tirofiban infusion in patients with unstable angina pectoris

    Directory of Open Access Journals (Sweden)

    Aliyev Emil

    2004-04-01

    Full Text Available Abstract Aim The inflammatory response, initiated by neutrophil and monocyte adhesion to endothelial cells, is important in the pathogenesis of acute coronary syndromes. Platelets play an important role in inflammatory process by interacting with monocytes and neutrophils. In this study, we investigated the effect of tirofiban on the levels of cell adhesion molecules (soluble intercellular adhesion molecule-1, sICAM-1, and vascular cell adhesion molecule-1, sVCAM-1 in patients with unstable angina pectoris (AP. Methods Thirty-five patients with unstable AP (Group I, ten patients with stable AP (Group II and ten subjects who had angiographycally normal coronary arteries (Group III were included the study. Group I was divided into two subgroups for the specific treatment regimens: Group IA (n = 15 received tirofiban and Group IB (n = 20 did not. Blood samples for investigating the cell adhesion molecules were drawn at zero time (baseline; 0 h in all patients and at 72 h in Group I. Results The baseline levels of sICAM-1 and sVCAM-1 were higher in Group I than in Groups II and III. They were higher in Group IA than in Group IB. However, the sICAM-1 and sVCAM-1 levels decreased significantly in Group IA after tirofiban infusion. In contrast, these levels remained unchanged or were increased above the baseline value in Group IB at 72 h. Conclusion The levels of cell adhesion molecules in patients with unstable AP decreased significantly after tirofiban infusion. Inhibition of platelet function by specific glycoprotein IIb/IIIa antagonists may decrease platelet-mediated inflammation and the ischemic end-point.

  10. Sugar cane bagasse prehydrolysis using hot water

    Directory of Open Access Journals (Sweden)

    D. Abril

    2012-03-01

    Full Text Available Results are presented on the hot water prehydrolysis of sugar cane bagasse for obtaining ethanol by fermentation. The experimental study consisted of the determination of the effect of temperature and time of prehydrolysis on the extraction of hemicelluloses, with the objective of selecting the best operating conditions that lead to increased yield of extraction with a low formation of inhibitors. The study, carried out in a pilot plant scale rotational digester, using a 3² experimental design at temperatures of 150-190ºC and times of 60-90 min, showed that it is possible to perform the hot water prehydrolysis process between 180-190ºC in times of 60-82 min, yielding concentrations of xylose > 35 g/L, furfural < 2.5 g/L, phenols from soluble lignin < 1.5 g/L, and concentrations < 3.0 g/L of hemicelluloses in the cellolignin residue. These parameters of temperature and prehydrolysis time could be used for the study of the later hydrolysis and fermentation stages of ethanol production from sugar cane bagasse.

  11. The Soluble Epoxide Hydrolase Inhibitor AR9281 Decreases Blood Pressure, Ameliorates Renal Injury and Improves Vascular Function in Hypertension

    Directory of Open Access Journals (Sweden)

    Sean Shaw

    2009-12-01

    Full Text Available Soluble epoxide hydrolase inhibitors (sEHIs are demonstrating promise as potential pharmaceutical agents for the treatment of cardiovascular disease, diabetes, inflammation, and kidney disease. The present study determined the ability of a first-inclass sEHI, AR9281, to decrease blood pressure, improve vascular function, and decrease renal inflammation and injury in angiotensin hypertension. Rats were infused with angiotensin and AR9281 was given orally during the 14-day infusion period. Systolic blood pressure averaged 180 ± 5 mmHg in vehicle treated and AR9281 treatment significantly lowered blood pressure to 142 ± 7 mmHg in angiotensin hypertension. Histological analysis demonstrated decreased injury to the juxtamedullary glomeruli. Renal expression of inflammatory genes was increased in angiotensin hypertension and two weeks of AR9281 treatment decreased this index of renal inflammation. Vascular function in angiotensin hypertension was also improved by AR9281 treatment. Decreased afferent arteriolar and mesenteric resistance endothelial dependent dilator responses were ameliorated by AR9281 treatment of angiotensin hypertensive rats. These data demonstrate that the first-in-class sEHI, AR9281, lowers blood pressure, improves vascular function and reduces renal damage in angiotensin hypertension.

  12. CCN activation of fumed silica aerosols mixed with soluble pollutants

    Science.gov (United States)

    Dalirian, M.; Keskinen, H.; Ahlm, L.; Ylisirniö, A.; Romakkaniemi, S.; Laaksonen, A.; Virtanen, A.; Riipinen, I.

    2014-09-01

    Particle-water interactions of completely soluble or insoluble particles are fairly well understood but less is known of aerosols consisting of mixtures of soluble and insoluble components. In this study, laboratory measurements were performed to investigate cloud condensation nuclei (CCN) activity of silica particles coated with ammonium sulphate (a salt), sucrose (a sugar) and bovine serum albumin known as BSA (a protein). In addition, the agglomerated structure of the silica particles was investigated by estimating the surface equivalent diameter based on measurements with a Differential Mobility Analyzer (DMA) and an Aerosol Particle Mass Analyzer (APM). By using the surface equivalent diameter the non-sphericity of the particles containing silica was accounted for when estimating CCN activation. Furthermore, characterizing critical supersaturations of particles consisting of pure soluble on insoluble compounds using existing frameworks showed that the CCN activation of single component particles was in good agreement with Köhler and adsorption theory based models when the agglomerated structure was accounted for. For mixed particles the CCN activation was governed by the soluble components, and the soluble fraction varied considerably with particle size for our wet-generated aerosols. Our results confirm the hypothesis that knowing the soluble fraction is the key parameter needed for describing the CCN activation of mixed aerosols, and highlight the importance of controlled coating techniques for acquiring a detailed understanding of the CCN activation of atmospheric insoluble particles mixed with soluble pollutants.

  13. Sugar recognition by human galactokinase

    Directory of Open Access Journals (Sweden)

    Timson David J

    2003-11-01

    Full Text Available Abstract Background Galactokinase catalyses the first committed step of galactose catabolism in which the sugar is phosphorylated at the expense of MgATP. Recent structural studies suggest that the enzyme makes several contacts with galactose – five side chain and two main chain hydrogen bonds. Furthermore, it has been suggested that inhibition of galactokinase may help sufferers of the genetic disease classical galactosemia which is caused by defects in another enzyme of the pathway galactose-1-phosphate uridyl transferase. Galactokinases from different sources have a range of substrate specificities and a diversity of kinetic mechanisms. Therefore only studies on the human enzyme are likely to be of value in the design of therapeutically useful inhibitors. Results Using recombinant human galactokinase expressed in and purified from E. coli we have investigated the sugar specificity of the enzyme and the kinetic consequences of mutating residues in the sugar-binding site in order to improve our understanding of substrate recognition by this enzyme. D-galactose and 2-deoxy-D-galactose are substrates for the enzyme, but N-acetyl-D-galactosamine, L-arabinose, D-fucose and D-glucose are all not phosphorylated. Mutation of glutamate-43 (which forms a hydrogen bond to the hydroxyl group attached to carbon 6 of galactose to alanine results in only minor changes in the kinetic parameters of the enzyme. Mutation of this residue to glycine causes a ten-fold drop in the turnover number. In contrast, mutation of histidine 44 to either alanine or isoleucine results in insoluble protein following expression in E. coli. Alteration of the residue that makes hydrogen bonds to the hydroxyl attached to carbons 3 and 4 (aspartate 46 results in an enzyme that although soluble is essentially inactive. Conclusions The enzyme is tolerant to small changes at position 2 of the sugar ring, but not at positions 4 and 6. The results from site directed mutagenesis could

  14. pH Regulation of Electrogenic Sugar/H+ Symport in MFS Sugar Permeases

    Science.gov (United States)

    Bazzone, Andre; Madej, M. Gregor; Kaback, H. Ronald

    2016-01-01

    Bacterial sugar symporters in the Major Facilitator Superfamily (MFS) use the H+ (and in a few cases Na+) electrochemical gradients to achieve active transport of sugar into the cell. Because a number of structures of MFS sugar symporters have been solved recently, molecular insight into the transport mechanism is possible from detailed functional analysis. We present here a comparative electrophysiological study of the lactose permease (LacY), the fucose permease (FucP) and the xylose permease (XylE), which reveals common mechanistic principles and differences. In all three symporters energetically downhill electrogenic sugar/H+ symport is observed. Comparison of the pH dependence of symport at symmetrical pH exhibits broad bell-shaped pH profiles extending over 3 to 6 pH units and a decrease at extremely alkaline pH ≥ 9.4 and at acidic to neutral pH = 4.6–7.5. The pH dependence can be described by an acidic to neutral apparent pK (pKapp) and an alkaline pKapp. Experimental evidence suggests that the alkaline pKapp is due to H+ depletion at the protonation site, while the acidic pKapp is due to inhibition of deprotonation. Since previous studies suggest that a single carboxyl group in LacY (Glu325) may be the only side chain directly involved in H+ translocation and a carboxyl side chain with similar properties has been identified in FucP (Asp46) and XylE (Asp27), the present results imply that the pK of this residue is switched during H+/sugar symport in all three symporters. PMID:27227677

  15. Impacto dos nutrientes N e K e de açúcares solúveis sobre populações de Diabrotica speciosa (Germar (Coleoptera, Chrysomelidae e Agrotis ipsilon (Hüfnagel (Lepidoptera, Noctuidae na cultura da batata, Solanum tuberosum L. (Solanaceae Impact of the nutrients N and K and soluble sugars on Diabrotica speciosa (Germar (Coleoptera, Chrysomelidae and Agrotis ipsilon (Hüfnagel (Lepidoptera, Noctuidae populations in potato crops, Solanum tuberosum L. (Solanaceae

    Directory of Open Access Journals (Sweden)

    Edson Henrique de Azeredo

    2004-03-01

    Full Text Available Foi estudada a ocorrência de Diabrotica speciosa (Germar, 1824 (Coleoptera, Chrysomelidae e de Agrotis ipsilon (Hüfnagel, 1767 (Lepidoptera, Noctuidae em plantas de batata, cultivares Achat e Monalisa, influenciadas por dosagens de nitrogênio e potássio, e teor mínimo de açúcares solúveis. Os seguintes parâmetros foram avaliados: concentração de nutrientes minerais e açúcar em folha verde, folha senescente, folha em abcisão, haste, tubérculo e planta total usando extratos de infusão em etanol 80%. A maior infestação por larvas de D. speciosa foi na cultivar Monalisa a 150 kg.ha-1 de N + K com 27,03% a PThe occurrence of Diabrotica speciosa (Germar, 1824 and Agrotis ipsilon (Hüfnagel, 1767 on the potato cultivars Achat and Monalisa, influenced by nitrogen and potassium dosage, and minimum theor of soluble sugars, was studied. The following parameters were evaluated: concentration of mineral nutrient and sugar in green leaf, senescent leaf, leaf in abscission, stem, tubercle and total plant using extracts of infusion in ethanol 80%. The largest infestation of D. speciosa larvae was on Monalisa cultivar at 150 kg.ha-1 of N + K with 27.03% at P< 0,05. It was observed that the effect of the dosage of N + K in the increment of the concentration of soluble sugars increased the damages in the tubercles and stems by A. ipsilon. The infestation by these species increased to 58.82% on the Monalisa cultivar, when the nitrogen dosage increased from zero to 150 kg.ha-1, in the absence of potassium. On the other hand, high dosage of K reduced the damages by A. ipsilon on Monalisa cultivar. However, it did not influence the storage of soluble sugar. The results indicated that in Achat cultivar the accumulated soluble sugar was reduced, probably sensibilized by elevation of potassic fertilization dosing, differing from Monalisa cultivar, in which the influence was by nitrogen dosing.

  16. Soluble Programmed Death 1 (PD-1) Is Decreased in Patients With Immune Thrombocytopenia (ITP): Potential Involvement of PD-1 Pathway in ITP Immunopathogenesis.

    Science.gov (United States)

    Birtas Atesoglu, Elif; Tarkun, Pinar; Demirsoy, Esra Terzi; Geduk, Ayfer; Mehtap, Ozgur; Batman, Adnan; Kaya, Fatih; Cekmen, Mustafa Baki; Gulbas, Zafer; Hacıhanefioglu, Abdullah

    2016-04-01

    Immune thrombocytopenia (ITP) is an autoimmune disease characterized by dysregulation of T cells. Programmed death (PD) 1 and programmed death 1 ligand 1 (PD-L1) are cosignaling molecules, and the major role of the PD-1 pathway is the inhibition of self-reactive T cells and to protect against autoimmune diseases. We measured levels of serum soluble PD 1 (sPD-1) and serum soluble PD-L1 (sPD-L1) in 67 patients with ITP (24 newly diagnosed ITP [ndITP], 43 chronic ITP [cITP]) and 21 healthy controls (HCs). We determined decreased serum sPD-1 levels both in patients with ndITP and in patients with cITP when compared to HC. Moreover, there was a positive correlation between sPD-1 levels and platelet counts. The sPD-L1 levels were decreased in patients with ndITP when compared to patients with cITP. This is the first study investigating PD-1 signaling pathway in ITP. Decreased sPD-1 levels may have a role in ITP pathogenesis as without the inhibitory regulation of PD-1, sustained activation of T cells may cause inflammatory responses which is the case in ITP. © The Author(s) 2014.

  17. Research on solubility characteristics of gaseous methyl iodide

    International Nuclear Information System (INIS)

    Zhou Yanmin; Sun Zhongning; Gu Haifeng; Wang Junlong

    2014-01-01

    With the deionized water as the absorbent, the solubility characteristics of the gaseous methyl iodide were studied under different temperature and pressure conditions, using a dynamic measuring method. The results show that within the range of experiment parameters, namely temperature is below 80℃ and pressure is lower than 0.3 MPa, the physical dissolution process of gaseous methyl iodide in water obeys Henry's law. The solubility coefficient under different temperature and pressure conditions was calculated based on the measurement results. Further research indicates that at atmospheric pressure, the solubility coefficient of methyl iodide in water decreases exponentially with the increase of temperature. While the pressure changes from 0.1 MPa to 0.3 MPa with equal interval, the solubility coefficient also increases linearly. The variation of the solubility coefficient with temperature under different pressure conditions all decreases exponentially. An equation is given to calculate the solubility coefficient of methyl iodide under different pressure and temperature conditions. (authors)

  18. Decreases in High-Fat and/or High-Added-Sugar Food Group Intake Occur when a Hypocaloric, Low-Fat Diet Is Prescribed Within a Lifestyle Intervention: A Secondary Cohort Analysis.

    Science.gov (United States)

    Sheikh, Vaishali Keshani; Raynor, Hollie A

    2016-10-01

    When a hypocaloric, low-fat diet is prescribed, intake of currently consumed foods can decrease, foods naturally low in fat and/or added sugar may increase, or fat- or sugar-modified foods may increase. To examine food group intake change and its relation to reductions in energy and fat intake and weight during a lifestyle intervention. Secondary cohort analysis. One hundred sixty-nine participants (aged 52.0±8.6 years, body mass index 34.9±4.5, 92% white, 97.6% non-Hispanic, and 56.8% women) with complete data at 0 and 6 months collected in a research setting. From three 24-hour telephone dietary recalls, 165 food groups from Nutrition Data System for Research software were coded into 25 larger food groups assessing intake of higher-fat and/or added-sugar food groups vs naturally lower-fat and/or added-sugar food groups and into 17 larger food groups assessing intake of nonmodified vs fat- and/or sugar-modified food groups. Repeated measures analyses of covariance (intervention group: covariate) assessed changes from 0 to 6 months. Hierarchical regressions examined changes in food group intake and changes in energy intake, percent energy from fat intake, and weight from 0 to 6 months. Significant reductions (Phypocaloric, low-fat diet is prescribed, reductions in high-fat and/or high-added-sugar food groups occur. Targeting reductions in high-fat meats may improve outcomes. Copyright © 2016 Academy of Nutrition and Dietetics. Published by Elsevier Inc. All rights reserved.

  19. Oral formulation strategies to improve solubility of poorly water-soluble drugs.

    Science.gov (United States)

    Singh, Abhishek; Worku, Zelalem Ayenew; Van den Mooter, Guy

    2011-10-01

    In the past two decades, there has been a spiraling increase in the complexity and specificity of drug-receptor targets. It is possible to design drugs for these diverse targets with advances in combinatorial chemistry and high throughput screening. Unfortunately, but not entirely unexpectedly, these advances have been accompanied by an increase in the structural complexity and a decrease in the solubility of the active pharmaceutical ingredient. Therefore, the importance of formulation strategies to improve the solubility of poorly water-soluble drugs is inevitable, thus making it crucial to understand and explore the recent trends. Drug delivery systems (DDS), such as solid dispersions, soluble complexes, self-emulsifying drug delivery systems (SEDDS), nanocrystals and mesoporous inorganic carriers, are discussed briefly in this review, along with examples of marketed products. This article provides the reader with a concise overview of currently relevant formulation strategies and proposes anticipated future trends. Today, the pharmaceutical industry has at its disposal a series of reliable and scalable formulation strategies for poorly soluble drugs. However, due to a lack of understanding of the basic physical chemistry behind these strategies, formulation development is still driven by trial and error.

  20. Biochemical characterisation and dietary fibre analysis of sugar beet supplemented cookies

    International Nuclear Information System (INIS)

    Pasha, I.; Jahangir, M.F.; Akhter, S.; Manzoor, M.S.

    2016-01-01

    This study was planned to utilize sugar beet powder as a rich source of dietary fibre in cookies. Purposely, five treatments namely T1, T2, T3, T4 and T5 with 4%, 8%, 12%, 16% and 20% sugar beet powder addition in wheat flour were chosen to estimate fibre, antioxidant profiling and engineering properties of cookies. Results showed an increased content of all above mentioned parameters. With the increment in sugar beet powder addition in treatments, dietary fibre analysis have shown that total dietary fibre (TDF), insoluble dietary fibre (IDF) and soluble dietary fibre (SDF) have depicted increasing trend with maximum for T5 for all dietary fibre types. Significant results were obtained for in vitro antioxidant studies including total phenolic content (TPC) and DPPH that showed increasing trend with T1 0.6 mg GAE/g and maximum values for T5 with 2.0 mg GAE/g for TPC and for DPPH with T5 being maximum value of 1.7% and minimum for T1 with 1.3%. T5 treatment with 20% sugar beet gave best physicochemical results but disturbed sensory properties while T3 with 12% sugar beet powder showed good physicochemical and sensory characteristics. Therefore, T3 with 12% level is considered as the best source of dietary fibre in bakery products and can be considered as the prospective choice to address metabolic syndromes. (author)

  1. Saccharification of recalcitrant biomass and integration options for lignocellulosic sugars from Catchlight Energy's sugar process (CLE Sugar).

    Science.gov (United States)

    Gao, Johnway; Anderson, Dwight; Levie, Benjamin

    2013-01-28

    Woody biomass is one of the most abundant biomass feedstocks, besides agriculture residuals in the United States. The sustainable harvest residuals and thinnings alone are estimated at about 75 million tons/year. These forest residuals and thinnings could produce the equivalent of 5 billion gallons of lignocellulosic ethanol annually. Softwood biomass is the most recalcitrant biomass in pretreatment before an enzymatic hydrolysis. To utilize the most recalcitrant lignocellulosic materials, an efficient, industrially scalable and cost effective pretreatment method is needed. Obtaining a high yield of sugar from recalcitrant biomass generally requires a high severity of pretreatment with aggressive chemistry, followed by extensive conditioning, and large doses of enzymes. Catchlight Energy's Sugar process, CLE Sugar, uses a low intensity, high throughput variation of bisulfite pulping to pretreat recalcitrant biomass, such as softwood forest residuals. By leveraging well-proven bisulfite technology and the rapid progress of enzyme suppliers, CLE Sugar can achieve a high yield of total biomass carbohydrate conversion to monomeric lignocellulosic sugars. For example, 85.8% of biomass carbohydrates are saccharified for un-debarked Loblolly pine chips (softwood), and 94.0% for debarked maple chips (hardwood). Furan compound formation was 1.29% of biomass feedstock for Loblolly pine and 1.10% for maple. At 17% solids hydrolysis of pretreated softwood, an enzyme dose of 0.075 g Sigma enzyme mixture/g dry pretreated (unwashed) biomass was needed to achieve 8.1% total sugar titer in the hydrolysate and an overall prehydrolysate liquor plus enzymatic hydrolysis conversion yield of 76.6%. At a much lower enzyme dosage of 0.044 g CTec2 enzyme product/g dry (unwashed) pretreated softwood, hydrolysis at 17% solids achieved 9.2% total sugar titer in the hydrolysate with an overall sugar yield of 85.0% in the combined prehydrolysate liquor and enzymatic hydrolysate. CLE Sugar has

  2. Sugar Sugar – don’t be misled / laat je niet misleiden

    NARCIS (Netherlands)

    Toebes, Brigit

    2017-01-01

    NRC Handelsblad’s Saturday 25 November issue contains an entry of eleven pages entirely devoted to sugar. It discusses a broad range of topics related to sugar, including the role of sugar throughout the centuries, sugar consumption in the Netherlands, the amount of sugar in bread, and sugar

  3. Blood sugar test

    Science.gov (United States)

    ... sugar; Blood sugar level; Fasting blood sugar; Glucose test; Diabetic screening - blood sugar test; Diabetes - blood sugar test ... The test may be done in the following ways: After you have not eaten anything for at least 8 ...

  4. A rapid review examining purchasing changes resulting from fiscal measures targeted at high sugar foods and sugar-sweetened drinks.

    Science.gov (United States)

    Roberts, Katharine E; Ells, Louisa J; McGowan, Victoria J; Machaira, Theodora; Targett, Victoria C; Allen, Rachel E; Tedstone, Alison E

    2017-12-15

    To aim of the review was to examine the most recent (2010 onwards) research evidence on the health and behavioural impacts, in adults and children, of fiscal strategies that target high sugar foods and sugar-sweetened drinks (SSDs). A pragmatic rapid review was undertaken using a systematic search strategy. The review was part of a programme of work to support policy development in relation to high sugar food and SSDs. A total of 11 primary research publications were included, describing evidence from France (n = 1), the Netherlands (n = 3), and the United States of America (n = 7), assessed through a variety of study designs, with the majority in adult populations (n = 10). The evidence reviewed focused on consumer behaviour outcomes and suggested that fiscal strategies can influence purchases of high sugar products. Although the majority of studies (n = 10), including three field studies, demonstrated that an increase in the price of high sugar foods and SSDs resulted in a decrease in purchases, eight studies were conducted in a laboratory or virtual setting which may not reflect real-life situations.Findings from this review support evidence from the broader literature that suggests that fiscal measures can be effective in influencing the purchasing of high sugar foods and SSDs.

  5. Hydrolysis technology for producing sugars from biomass as raw material for the chemical industry - SugarTech

    Energy Technology Data Exchange (ETDEWEB)

    Siika-aho, M.; Kallioinen, A.; Pakula, T. (and others) (VTT Technical Research Centre of Finland, Espoo (Finland)), email: matti.siika-aho@vtt.fi

    2009-10-15

    In SugarTech project, spruce, forest residue, birch and sugar cane bagasse have been studied as a raw material for production of sugars to be processed further to ethanol and other chemicals. These raw materials containing high proportion of carbohydrates have been analysed and pretreated for enzyme hydrolysis by steam explosion and oxidative methods. The pretreated materials have been studied in respect to yield and enzymatic hydrolysability. Birch and bagasse could easily be pretreated with steam explosion. Catalytic oxidation treatment of spruce produced material with superior hydrolysability to steam exploded material. Enzyme adsorption and desorption were studied aiming at recycling of enzymes in the hydrolysis process. Purified cellulase enzymes were found to have high tendency to adsorption on lignocellulosic substrate. Adsorption could be decreased by additives, e.g. urea and BSA. In addition, the hydrolytic system of Trichoderma reesei in the presence of different substrates has been studied. (orig.)

  6. 76 FR 62339 - Domestic Sugar Program-2011-Crop Cane Sugar and Beet Sugar Marketing Allotments and Company...

    Science.gov (United States)

    2011-10-07

    ... DEPARTMENT OF AGRICULTURE Commodity Credit Corporation Domestic Sugar Program--2011-Crop Cane Sugar and Beet Sugar Marketing Allotments and Company Allocations AGENCY: Commodity Credit Corporation... the fiscal year (FY) 2012 State sugar marketing allotments and company allocations to sugarcane and...

  7. Head-To-Head Comparison of Different Solubility-Enabling Formulations of Etoposide and Their Consequent Solubility-Permeability Interplay.

    Science.gov (United States)

    Beig, Avital; Miller, Jonathan M; Lindley, David; Carr, Robert A; Zocharski, Philip; Agbaria, Riad; Dahan, Arik

    2015-09-01

    The purpose of this study was to conduct a head-to-head comparison of different solubility-enabling formulations, and their consequent solubility-permeability interplay. The low-solubility anticancer drug etoposide was formulated in several strengths of four solubility-enabling formulations: hydroxypropyl-β-cyclodextrin, the cosolvent polyethylene glycol 400 (PEG-400), the surfactant sodium lauryl sulfate, and an amorphous solid dispersion formulation. The ability of these formulations to increase the solubility of etoposide was investigated, followed by permeability studies using the parallel artificial membrane permeability assay (PAMPA) and examination of the consequent solubility-permeability interplay. All formulations significantly increased etoposide's apparent solubility. The cyclodextrin-, surfactant-, and cosolvent-based formulations resulted in a concomitant decreased permeability that could be modeled directly from the proportional increase in the apparent solubility. On the contrary, etoposide permeability remained constant when using the ASD formulation, irrespective of the increased apparent solubility provided by the formulation. In conclusion, supersaturation resulting from the amorphous form overcomes the solubility-permeability tradeoff associated with other formulation techniques. Accounting for the solubility-permeability interplay may allow to develop better solubility-enabling formulations, thereby maximizing the overall absorption of lipophilic orally administered drugs. © 2015 Wiley Periodicals, Inc. and the American Pharmacists Association.

  8. Noble gases solubility in water

    International Nuclear Information System (INIS)

    Crovetto, Rosa; Fernandez Prini, Roberto.

    1980-07-01

    The available experimental data of solubility of noble gases in water for temperatures smaller than 330 0 C have been critically surveyed. Due to the unique structure of the solvent, the solubility of noble gases in water decreases with temperature passing through a temperature of minimum solubility which is different for each gas, and then increases at higher temperatures. As aresult of the analysis of the experimental data and of the features of the solute-solvent interaction, a generalized equation is proposed which enables thecalculation of Henry's coefficient at different temperatures for all noble gases. (author) [es

  9. Nectar Sugar Modulation and Cell Wall Invertases in the Nectaries of Day- and Night- Flowering Nicotiana.

    Science.gov (United States)

    Tiedge, Kira; Lohaus, Gertrud

    2018-01-01

    Nectar composition varies between species, depending on flowering time and pollinator type, among others. Various models of the biochemical and molecular mechanisms underlying nectar production and secretion have been proposed. To gain insights into these mechanisms, day- and night-flowering tobacco ( Nicotiana ) species with high or low proportions of hexoses in the nectar were analyzed. Nectar and nectaries were simultaneously collected, throughout the day and night. Soluble sugars and starch were determined and the activity and expression level of cell wall invertase (CW-INVs) were measured in nectaries. Nectaries and nectar of the five Nicotiana species contained different amounts of sucrose, glucose, and fructose. CW-INV activity was detected in the nectaries of all Nicotiana species and is probably involved in the hydrolysis of sucrose in the nectary tissue and during nectar secretion. The larger differences in the sucrose-to-hexose-ratio between nectaries and nectar in diurnal species compared to nocturnal species can be explained by higher sucrose cleavage within the nectaries in night-flowering species, and during secretion in day-flowering species. However, cell wall invertase alone cannot be responsible for the differences in sugar concentrations. Within the nectaries of the Nicotiana species, a portion of the sugars is transiently stored as starch. In general, night-flowering species showed higher starch contents in the nectaries compared to day-flowering species. Moreover, in night flowering species, the starch content decreased during the first half of the dark period, when nectar production peaks. The sucrose concentrations in the cytoplasm of nectarial cells were extrapolated from nectary sucrose contents. In day-flowering species, the sucrose concentration in the nectary cytoplasm was about twice as high as in nectar, whereas in night-flowering species the situation was the opposite, which implies different secretion mechanisms. The secreted nectar

  10. BIOACTIVE COMPOUNDS IN CONVENTIONAL AND NO ADDED SUGARS RED STRAWBERRY GUAVA (Psidium cattleianum Sabine JELLIES

    Directory of Open Access Journals (Sweden)

    GABRIELA NIEMEYER REISSIG

    Full Text Available ABSTRACT This study aimed to prepare jellies of conventional type of red strawberry guava (with added sucrose and no added sugar and evaluate the physical and chemical composition and content of bioactive compounds in them. Four jellies formulations were prepared: conventional with addition of sucrose (F1, aspartame (F2, saccharin and cyclamate (F3, acesulfame and sucralose (F4. Physicochemical analysis of pH were carried out, as well as analysis of titratable acidity, total soluble solids, ashes, proteins, lipids, moisture, carbohydrates, calories, lightness, color tone, total phenols, anthocyanins, carotenoids, ascorbic acid and antioxidant activity, by the capture of DPPH and ABTS radicals. Conventional and no added sugars jellies did not differ for total phenols, total anthocyanins and ascorbic acid. However, processing exerted significant influence (p=0.05 on total carotenoids and antioxidant activity. It is feasible to use red strawberry guava for the preparation of conventional and no added sugar jellies. The products, however, show a significant difference in carotenoids content, with the highest content of these and higher antioxidant activity in processed jellies without sugars addition.

  11. N-succinyl-chitosan as a drug carrier: water-insoluble and water-soluble conjugates.

    Science.gov (United States)

    Kato, Yoshinori; Onishi, Hiraku; Machida, Yoshiharu

    2004-02-01

    N-succinyl-chitosan (Suc-Chi) has favourable properties as a drug carrier such as biocompatibility, low toxicity and long-term retention in the body. It was long retained in the systemic circulation after intravenous administration, and the plasma half-lives of Suc-Chi (MW: 3.4 x 10(5); succinylation degree: 0.81 mol/sugar unit; deacetylation degree: 1.0 mol/sugar unit) were ca. 100.3h in normal mice and 43 h in Sarcoma 180-bearing mice. The biodistribution of Suc-Chi into other tissues was trace apart from the prostate and lymph nodes. The maximum tolerable dose for the intraperitoneal injection of Suc-Chi to mice was greater than 2 g/kg. The water-insoluble and water-soluble conjugates could be prepared using a water-soluble carbodiimide and mitomycin C (MMC) or using an activated ester of glutaric MMC. In vitro release characteristics of these conjugates showed similar patterns, i.e. a pH-dependent manner, except that water-insoluble conjugates showed a slightly slower release of MMC than water-soluble ones. The conjugates of MMC with Suc-Chi showed good antitumour activities against various tumours such as murine leukaemias (L1210 and P388), B16 melanoma, Sarcoma 180 solid tumour, a murine liver metastatic tumour (M5076) and a murine hepatic cell carcinoma (MH134). This review summarizes the utilization of Suc-Chi as a drug carrier for macromolecular conjugates of MMC and the therapeutic efficacy of the conjugates against various tumours.

  12. High sugar and butter (HSB) diet induces obesity and metabolic syndrome with decrease in regulatory T cells in adipose tissue of mice.

    Science.gov (United States)

    Maioli, Tatiani Uceli; Gonçalves, Juliana Lauar; Miranda, Mariana Camila Gonçalves; Martins, Vinícius Dantas; Horta, Laila Sampaio; Moreira, Thais Garcias; Godard, Ana Lucia Brunialti; Santiago, Andrezza Fernanda; Faria, Ana Maria Caetano

    2016-02-01

    The purpose of the study was to develop a novel diet based on standard AIN93G diet that would be able to induce experimental obesity and impair immune regulation with high concentrations of both carbohydrate and lipids. To compare the effects of this high sugar and butter (HSB) diet with other modified diets, male C57BL/6 mice were fed either mouse chow, or AIN93G diet, or high sugar (HS) diet, or high-fat (HF) diet, or high sugar and butter (HSB) diet for 11 weeks ad libitum. HSB diet induced higher weight gain. Therefore, control AIN93G and HSB groups were chosen for additional analysis. Regulatory T cells were studied by flow cytometry, and cytokine levels were measured by ELISA. Although HF and HSB diets were able to induce a higher weight gain compatible with obesity in treated mice, HSB-fed mice presented the higher levels of serum glucose after fasting and the lowest frequency of regulatory T cells in adipose tissue. In addition, mice that were fed HSB diet presented higher levels of cholesterol and triglycerides, hyperleptinemia, increased resistin and leptin levels as well as reduced adiponectin serum levels. Importantly, we found increased frequency of CD4(+)CD44(+) effector T cells, reduction of CD4(+)CD25(+)Foxp3(+) and Th3 regulatory T cells as well as decreased levels of IL-10 and TGF-β in adipose tissue of HSB-fed mice. Therefore, HSB represents a novel model of obesity-inducing diet that was efficient in triggering alterations compatible with metabolic syndrome as well as impairment in immune regulatory parameters.

  13. High-sugar intake does not exacerbate metabolic abnormalities or cardiac dysfunction in genetic cardiomyopathy.

    Science.gov (United States)

    Hecker, Peter A; Galvao, Tatiana F; O'Shea, Karen M; Brown, Bethany H; Henderson, Reney; Riggle, Heather; Gupte, Sachin A; Stanley, William C

    2012-05-01

    A high-sugar intake increases heart disease risk in humans. In animals, sugar intake accelerates heart failure development by increased reactive oxygen species (ROS). Glucose-6-phosphate dehydrogenase (G6PD) can fuel ROS production by providing reduced nicotinamide adenine dinucleotide phosphate (NADPH) for superoxide generation by NADPH oxidase. Conversely, G6PD also facilitates ROS scavenging using the glutathione pathway. We hypothesized that a high-sugar intake would increase flux through G6PD to increase myocardial NADPH and ROS and accelerate cardiac dysfunction and death. Six-week-old TO-2 hamsters, a non-hypertensive model of genetic cardiomyopathy caused by a δ-sarcoglycan mutation, were fed a long-term diet of high starch or high sugar (57% of energy from sucrose plus fructose). After 24 wk, the δ-sarcoglycan-deficient animals displayed expected decreases in survival and cardiac function associated with cardiomyopathy (ejection fraction: control 68.7 ± 4.5%, TO-2 starch 46.1 ± 3.7%, P sugar 58.0 ± 4.2%, NS, versus TO-2 starch or control; median survival: TO-2 starch 278 d, TO-2 sugar 318 d, P = 0.133). Although the high-sugar intake was expected to exacerbate cardiomyopathy, surprisingly, there was no further decrease in ejection fraction or survival with high sugar compared with starch in cardiomyopathic animals. Cardiomyopathic animals had systemic and cardiac metabolic abnormalities (increased serum lipids and glucose and decreased myocardial oxidative enzymes) that were unaffected by diet. The high-sugar intake increased myocardial superoxide, but NADPH and lipid peroxidation were unaffected. A sugar-enriched diet did not exacerbate ventricular function, metabolic abnormalities, or survival in heart failure despite an increase in superoxide production. Copyright © 2012 Elsevier Inc. All rights reserved.

  14. Glycogen synthase activation by sugars in isolated hepatocytes.

    Science.gov (United States)

    Ciudad, C J; Carabaza, A; Bosch, F; Gòmez I Foix, A M; Guinovart, J J

    1988-07-01

    We have investigated the activation by sugars of glycogen synthase in relation to (i) phosphorylase a activity and (ii) changes in the intracellular concentration of glucose 6-phosphate and adenine nucleotides. All the sugars tested in this work present the common denominator of activating glycogen synthase. On the other hand, phosphorylase a activity is decreased by mannose and glucose, unchanged by galactose and xylitol, and increased by tagatose, glyceraldehyde, and fructose. Dihydroxyacetone exerts a biphasic effect on phosphorylase. These findings provide additional evidence proving that glycogen synthase can be activated regardless of the levels of phosphorylase a, clearly establishing that a nonsequential mechanism for the activation of glycogen synthase occurs in liver cells. The glycogen synthase activation state is related to the concentrations of glucose 6-phosphate and adenine nucleotides. In this respect, tagatose, glyceraldehyde, and fructose deplete ATP and increase AMP contents, whereas glucose, mannose, galactose, xylitol, and dihydroxyacetone do not alter the concentration of these nucleotides. In addition, all these sugars, except glyceraldehyde, increase the intracellular content of glucose 6-phosphate. The activation of glycogen synthase by sugars is reflected in decreases on both kinetic constants of the enzyme, M0.5 (for glucose 6-phosphate) and S0.5 (for UDP-glucose). We propose that hepatocyte glycogen synthase is activated by monosaccharides by a mechanism triggered by changes in glucose 6-phosphate and adenine nucleotide concentrations which have been described to modify glycogen synthase phosphatase activity. This mechanism represents a metabolite control of the sugar-induced activation of hepatocyte glycogen synthase.

  15. The solubility-permeability interplay and its implications in formulation design and development for poorly soluble drugs.

    Science.gov (United States)

    Dahan, Arik; Miller, Jonathan M

    2012-06-01

    While each of the two key parameters of oral drug absorption, the solubility and the permeability, has been comprehensively studied separately, the relationship and interplay between the two have been largely ignored. For instance, when formulating a low-solubility drug using various solubilization techniques: what are we doing to the apparent permeability when we increase the solubility? Permeability is equal to the drug's diffusion coefficient through the membrane times the membrane/aqueous partition coefficient divided by the membrane thickness. The direct correlation between the intestinal permeability and the membrane/aqueous partitioning, which in turn is dependent on the drug's apparent solubility in the GI milieu, suggests that the solubility and the permeability are closely associated, exhibiting a certain interplay between them, and the current view of treating the one irrespectively of the other may not be sufficient. In this paper, we describe the research that has been done thus far, and present new data, to shed light on this solubility-permeability interplay. It has been shown that decreased apparent permeability accompanies the solubility increase when using different solubilization methods. Overall, the weight of the evidence indicates that the solubility-permeability interplay cannot be ignored when using solubility-enabling formulations; looking solely at the solubility enhancement that the formulation enables may be misleading with regards to predicting the resulting absorption, and hence, the solubility-permeability interplay must be taken into account to strike the optimal solubility-permeability balance, in order to maximize the overall absorption.

  16. Old oil palm trunk: A promising source of sugars for bioethanol production

    Energy Technology Data Exchange (ETDEWEB)

    Yamada, H.; Ohara, S. [Department of Global Agricultural Sciences, University of Tokyo, 1-1-1, Yayoi, Bunkyo 113-8657 (Japan); Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki 305-8687 (Japan); Tanaka, R.; Yamamoto, K. [Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki 305-8687 (Japan); Sulaiman, O.; Hashim, R.; Hamid, Z.A.A.; Yahya, M.K.A. [School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang (Malaysia); Kosugi, A.; Arai, T.; Murata, Y.; Nirasawa, S. [Japan International Research Center for Agricultural Sciences, 1-1, Owashi, Tsukuba, Ibaraki 305-8686 (Japan); Mohd Yusof, Mohd Nor; Ibrahim, Wan Asma [Forest Research Institute Malaysia (FRIM), Kepong, 52109 Selangor (Malaysia); Mori, Y. [Department of Global Agricultural Sciences, University of Tokyo, 1-1-1, Yayoi, Bunkyo 113-8657 (Japan); Japan International Research Center for Agricultural Sciences, 1-1, Owashi, Tsukuba, Ibaraki 305-8686 (Japan)

    2010-11-15

    Oil palm trees are replanted at an interval of approximately 25 years because of decreased oil productivity of old trees. Consequently the felled trunks are the enormous amount of biomass resources in the palm oil producing countries such as Malaysia and Indonesia. In this report, we found that the felled oil palm trunk contains large quantity of sap, which accounts for approximately 70% of the whole trunk weight, and that sugars existing in the sap increased remarkably during storage after logging. Total sugar in the sap increased from 83 mg ml{sup -1} to 153 mg ml{sup -1}, the concentration comparable to that of sugar cane juice, after 30 days of storage, followed by the gradual decrease. The sugars contained in the sap were glucose, sucrose, fructose and galactose, all of which are fermentable by ordinary industrial yeast strains. The results indicate that old oil palm trunk becomes a promising source of sugars by proper aging after logging and, thus, its sap can be a good feedstock for bioethanol. (author)

  17. Improving the de-agglomeration and dissolution of a poorly water soluble drug by decreasing the agglomerate strength of the cohesive powder.

    Science.gov (United States)

    Allahham, Ayman; Stewart, Peter J; Das, Shyamal C

    2013-11-30

    Influence of ternary, poorly water-soluble components on the agglomerate strength of cohesive indomethacin mixtures during dissolution was studied to explore the relationship between agglomerate strength and extent of de-agglomeration and dissolution of indomethacin (Ind). Dissolution profiles of Ind from 20% Ind-lactose binary mixtures, and ternary mixtures containing additional dibasic calcium phosphate (1% or 10%; DCP), calcium sulphate (10%) and talc (10%) were determined. Agglomerate strength distributions were estimated by Monte Carlo simulation of particle size, work of cohesion and packing fraction distributions. The agglomerate strength of Ind decreased from 1.19 MPa for the binary Ind mixture to 0.84 MPa for 1DCP:20Ind mixture and to 0.42 MPa for 1DCP:2Ind mixture. Both extent of de-agglomeration, demonstrated by the concentration of the dispersed indomethacin distribution, and extent of dispersion, demonstrated by the particle size of the dispersed indomethacin, were in descending order of 1DCP:2Ind>1DCP:20Ind>binary Ind. The addition of calcium sulphate dihydrate and talc also reduced the agglomerate strength and improved de-agglomeration and dispersion of indomethacin. While not definitively causal, the improved de-agglomeration and dispersion of a poorly water soluble drug by poorly water soluble components was related to the agglomerate strength of the cohesive matrix during dissolution. Copyright © 2013 Elsevier B.V. All rights reserved.

  18. Enzymatic synthesis of hydrophilic undecylenic acid sugar esters and their biodegradability.

    Science.gov (United States)

    Raku, Takao; Kitagawa, Masaru; Shimakawa, Hiromi; Tokiwa, Yutaka

    2003-01-01

    To enhance water solubility of 10-undecylenic acid, which has anti-fungus, anti-bacterial and anti-virus activity, D-glucose, trehalose and sucrose were regioselectively esterified with vinyl 10-undecylenic acid ester in dimethyl formamide by a commercial protease, Bioprase conc., from Bacillus subtilis. 6-O-(10-Undecylenoyl) D-glucose, 6-O-(10-undecylenoyl) trehalose and 1'-O-(10-undecylenoyl) sucrose were obtained. The influence of structural variation by changing the sugar moiety was analyzed the surface tension and biodegradability.

  19. Impacts of Sugar Import Policy on Sugar Production in Indonesia

    OpenAIRE

    Suryantoro, Agustinus; Susilo, Albertus Magnus; Supriyono, Supriyono

    2013-01-01

    Production of sugar unful lled consumption of Indonesia society. The lack of consumption and productionhave ful lled by import. Assumption national consumption 2,7 million ton, Indonesia will import sugar in 2013predicted about 300.000 ton (Tempo.co, August, 21, 2012).The aims in general of this research are to understand the impact of sugar import policy on sugar production.Especially (1) to understand the factors that in uence sugar import price, (2) to understand impact of sugarimport pric...

  20. On solubility of rare earth chlorides in water at different temperatures

    International Nuclear Information System (INIS)

    Nikolaev, A.V.; Sorokina, A.A.; Sokolova, N.P.; Kotlyar-Shapirov, G.S.; Bagryantseva, L.I.

    1978-01-01

    Solubility of rare earth chlorides at -5, -10 and -15 deg C is studied. Rare earth chloride solubility dependences on the temperature in the interval from -15 to 50 deg C are presented. Decrease of solubility temperature coefficient to a zero is observed at temperature drop almost for all rare earth chlorides. Solubility temperature coefficient at the same temperature but for different rare earth chlorides reduces appreciably with the growth of rare earth chloride serial number. This testifies to the corresponding decrease of integral solution heat of rare earth chloride crystallohydrates

  1. Dental Caries Level and Sugar Consumption in 12-Year-Old Children from Poland.

    Science.gov (United States)

    Olczak-Kowalczyk, Dorota; Turska, Anna; Gozdowski, Dariusz; Kaczmarek, Urszula

    2016-01-01

    The frequent and high consumption of sugar products, particularly sucrose, is one of the causative factors of dental caries. Meta-analyses assessing the relationship between sugar intake and dental caries revealed that a restricted sugar intake to less than 10% of the daily energy intake results in substantial health benefits. Sugar consumption in Poland is 2-fold higher than recommended by the WHO. As change in dietary habits is slow, knowledge of whether a gradual reduction of sugar consumption influences beneficially the dental condition is important. Assessment of the relationship between caries experience and sugar consumption in 12-year-old children. The data obtained from the Statistical Agricultural Yearbooks of the Central Statistical Office in Poland regarding the average yearly sugar intake by a person in the years 1995-2013, and caries prevalence (frequency and DMFT) resulting from the national epidemiological studies of the 12-year-old children conducted by the Ministry of Health in those years were analyzed. The data was analyzed by linear regression. Regression function parameters and coefficients of determination were assessed for a possible link between sugar consumption and dental caries frequency and severity was expressed as DMFT value. The mean yearly sugar intake by a statistical Pole ranged from 43.6 kg (2002) to 35.3 kg (2006). Despite a slight trend to lower the sugar consumption, its mean intake in 1995 and 2013 was the same (41.9 kg). Caries frequency and DMFT decreased in 2012 compared to 1995 from 90.5% to 79.6% and from 4.3 to 3.53 kg in 2012, respectively. The increased sugar intake by 1 kg/year caused the increase of caries frequency by 1% and DMFT value by 0.2. Even a relatively low decrease in sugar consumption can exert some beneficial influence on the dental condition in adolescents, particularly upon the severity of caries.

  2. Decreasing DOC trends in soil solution along the hillslopes at two IM sites in southern Sweden--geochemical modeling of organic matter solubility during acidification recovery.

    Science.gov (United States)

    Löfgren, Stefan; Gustafsson, Jon Petter; Bringmark, Lage

    2010-12-01

    Numerous studies report increased concentrations of dissolved organic carbon (DOC) during the last two decades in boreal lakes and streams in Europe and North America. Recently, a hypothesis was presented on how various spatial and temporal factors affect the DOC dynamics. It was concluded that declining sulphur deposition and thereby increased DOC solubility, is the most important driver for the long-term DOC concentration trends in surface waters. If this recovery hypothesis is correct, the DOC levels should increase both in the soil solution as well as in the surrounding surface waters as soil pH rises and the ionic strength declines due to the reduced input of SO(4)(2-) ions. In this project a geochemical model was set up to calculate the net humic charge and DOC solubility trends in soils during the period 1996-2007 at two integrated monitoring sites in southern Sweden, showing clear signs of acidification recovery. The Stockholm Humic Model was used to investigate whether the observed DOC solubility is related to the humic charge and to examine how pH and ionic strength influence it. Soil water data from recharge and discharge areas, covering both podzols and riparian soils, were used. The model exercise showed that the increased net charge following the pH increase was in many cases counteracted by a decreased ionic strength, which acted to decrease the net charge and hence the DOC solubility. Thus, the recovery from acidification does not necessarily have to generate increasing DOC trends in soil solution. Depending on changes in pH, ionic strength and soil Al pools, the trends might be positive, negative or indifferent. Due to the high hydraulic connectivity with the streams, the explanations to the DOC trends in surface waters should be searched for in discharge areas and peat lands. Copyright © 2010 Elsevier B.V. All rights reserved.

  3. Water relations link carbon and oxygen isotope discrimination to phloem sap sugar concentration in eucalyptus globulus

    International Nuclear Information System (INIS)

    Cernusak, L.A.; Farquhar, G.D.; Arthur, D.J; Pate, J.S.

    2002-01-01

    Full text: The carbon isotope ratio of phloem sap sugars has been previously observed to correlate strongly with the phloem sap sugar concentration in Eucalyptus globulus. We hypothesized that the correspondence between these two parameters results from co-linearity in their responses to variation in plant water potential. Carbon isotope discrimination is expected to decrease with decreasing plant water potential due to the influence of stomatal conductance on the ratio of intercellular to ambient CO 2 , concentrations (c 1 /c a ). Conversely, we expected the phloem sap sugar concentration to increase with decreasing plant water potential, thereby maintaining positive turgor pressure within the sieve tubes. The study comprised 40 individual Eucalyptus globulus trees growing in three plantations situated on opposing ends of a rainfall gradient in southwestern Australia. A strong correlation was observed between the carbon isotope ratio in phloem sap sugars and phloem sap sugar concentration. Carbon isotope discrimination correlated positively with shoot water potential, whereas phloem sap sugar concentration correlated negatively with shoot water potential. The relationship between carbon isotope discrimination measured in phloem sap sugars collected from the stem and c 1 /c a measured instantaneously on subtending leaves was close to that theoretically predicted. Accordingly, a strong, negative relationship was observed between instantaneous c 1 /c a and the phloem sap sugar concentration. Oxygen isotope discrimination in phloem sap sugars also correlated strongly with phloem sap sugar concentration. A theoretical model suggested that the observed variation in stomatal conductance was sufficient to account for the variation observed in oxygen isotope discrimination across the study. Results strongly support the contention that water relations form a mechanistic link between phloem sap sugar concentration and both instantaneous and integrated measures of the

  4. The ultrasound-assisted sugar extraction from sugar beet cossettes

    International Nuclear Information System (INIS)

    Stasiak, D.M.

    2005-01-01

    The aim of this work was to study the ultrasound-assisted water extraction of sugar from sugar beet cossettes. The ultrasound bath device (25 kHz, 200 W) was used. The sonication accelerated sugar diffusion at both temperatures 18 deg C and 77.6 deg C and gave the higher level of dry matter content SS (4-6 percent) and sugar content CK (7-22 percent) in juice. The SS and CK depended on time of exposition, time and temperature of extraction. In particular, the effects of 5 min ultrasound-assisted extraction were equal to 20 min extraction in traditional conditions. The shorter time, lower temperature, higher efficiency and purity of juice could be the effects of sugar extraction with ultrasound. The change of thickness of diffusion membrane, microflows in tissue as well as it's environment caused by ultrasound was the reason of acceleration of sugar extraction

  5. Role of chlorophylls, amino acids and sugars in tea

    International Nuclear Information System (INIS)

    Dev Choudhury, M.N.

    1980-01-01

    Plucked tea shoots from clones of different varieties of tea were withered, rolled, fermented and fired by CTC and orthodox methods of manufacture. Quantitative changes in the levels of chlorophylls, amino acids and water soluble sugars during different stages of processing of tea and also changes in the contents of their degradation products were studied by feeding 14 C-labelled phenylalanine, glucose, sodium carbonate and sodium propionate to the excised shoots and subsequently analysing the products. Results are discussed and suggestions have been made about adjusting the conditions of manufacture so that the teas with desired chemical constituents are produced. (M.G.B.)

  6. Solubility of Nd in brine

    International Nuclear Information System (INIS)

    Khalili, F.I.; Symeopoulos, V.; Chen, J.F.; Choppin, G.R.

    1994-01-01

    The solubility of Nd(III) has been measured at 23±3 C in a synthetic brine at pcH 6.4, 8.4, 10.4 and 12.4. The brine consisted predominantly of (Na+K)Cl and MgCl 2 with an ionic strength of 7.8 M (9.4 m) a solid compound of Nd(III) at each pcH was assigned from X-ray diffraction patterns. The log values of the experimental solubilities decrease fomr -3 at pcH 6.4 to -5.8 at pcH 8.4; at pcH 10.4 and 12.4 the solubility was below the detection limit of -7.5. The experimental solubility does not follow closely the variation with pcH estimated from modeling of the species in solution in equilibrium with the Nd solid using S.I.T. (orig.)

  7. Ozone impact on vegetation: A primary lesion in sugar export from source leaves

    Energy Technology Data Exchange (ETDEWEB)

    Grantz, D.A.

    1999-07-01

    Ozone (O{sub 3}) remains a serious threat to native and agricultural vegetation. In many plants, including Pima cotton (Gossypium barbadense L.), O{sub 3} reduces development of efficient root systems. To investigate the mechanism of O{sub 3}-inhibited allocation of biomass to roots, leaves were exposed to 45 min pulses of O{sub 3} ranging from 0.0 to 0.8 {micro}l 1{sup 11} followed by a 15 min pulse of {sup 14}CO{sub 2}. The rapid phase of export of {sup 14}C-carbohydrate from source leaves was monitored with a Geiger Muller Tube. A single compartment model with a single exponential decay function yielded first order rate constants. O{sub 3} retarded efflux, decreased the rate constant, and increased the calculated soluble sugar pool remaining in the source leaves. With incorporation of an asymptote into the single exponential model, equivalent to the label remaining in the leaf at the end of a prolonged photoperiod, calculated rate constants and sugar contents for O{sub 3}-free control leaves were similar to values from the literature. Total carbohydrate transported from source leaves and thus available for export to the roots was reduced by O{sub 3} effects on assimilation (up to 20%) and O{sub 3} effects on efflux (up to 70%). O{sub 3}-inhibition of root system development is therefore dominated by the impact on phloem translocation rather than by effects on carbon assimilation.

  8. Solubility of xenon in amino-acid solutions. II. Nine less-soluble amino acids

    Science.gov (United States)

    Kennan, Richard P.; Himm, Jeffrey F.; Pollack, Gerald L.

    1988-05-01

    Ostwald solubility (L) of xenon gas, as the radioisotope 133Xe, has been measured as a function of solute concentration, at 25.0 °C, in aqueous solutions of nine amino acids. The amino-acid concentrations investigated covered much of their solubility ranges in water, viz., asparagine monohydrate (0-0.19 M), cysteine (0-1.16 M), glutamine (0-0.22 M), histidine (0-0.26 M), isoleucine (0-0.19 M), methionine (0-0.22 M), serine (0-0.38 M), threonine (0-1.4 M), and valine (0-0.34 M). We have previously reported solubility results for aqueous solutions of six other, generally more soluble, amino acids (alanine, arginine, glycine, hydroxyproline, lysine, and proline), of sucrose and sodium chloride. In general, L decreases approximately linearly with increasing solute concentration in these solutions. If we postulate that the observed decreases in gas solubility are due to hydration, the results under some assumptions can be used to calculate hydration numbers (H), i.e., the number of H2O molecules associated with each amino-acid solute molecule. The average values of hydration number (H¯) obtained at 25.0 °C are 15.3±1.5 for asparagine, 6.8±0.3 for cysteine, 11.5±1.1 for glutamine, 7.3±0.7 for histidine, 5.9±0.4 for isoleucine, 10.6±0.8 for methionine, 11.2±1.3 for serine, 7.7± 1.0 for threonine, and 6.6±0.6 for valine. We have also measured the temperature dependence of solubility L(T) from 5-40 °C for arginine, glycine, and proline, and obtained hydration numbers H¯(T) in this range. Between 25-40 °C, arginine has an H¯ near zero. This may be evidence for an attractive interaction between xenon and arginine molecules in aqueous solution.

  9. Amino acid digestibility of corn distillers dried grains with solubles, liquid condensed solubles, pulse dried thin stillage, and syrup balls fed to growing pigs.

    Science.gov (United States)

    Soares, J A; Stein, H H; Singh, V; Shurson, G S; Pettigrew, J E

    2012-04-01

    Distillers dried grains with solubles (DDGS) has low and variable AA digestibility. The variability is often attributed to damage during the heating process, and it has been suggested that the damage happens to the soluble components of DDGS such as reducing sugars. Combining solubles and grains sometimes produces syrup balls (SB); their digestibility is unknown. The objective of this experiment was to identify potential sources of poor and variable AA digestibility in DDGS. Specifically, our objective was to determine whether the problems are associated with the solubles component or with SB. The ingredients evaluated were DDGS, intact SB, ground SB, liquid condensed solubles (LCS), and pulse dried thin stillage (PDTS) obtained from the same ethanol plant. The LCS is produced by evaporation of thin stillage. Each ingredient was used as the only source of AA in an experimental diet. In a duplicate 6 × 6 Latin square design with 7-d adaptation and collection periods, the 6 treatments consisted of an N-free diet and the 5 test ingredients. Pigs had 5 d of adaptation to each diet, and on d 6 and 7 ileal digesta were collected from an ileal cannula for 8 h each day. Both SB treatments had apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of AA that were similar or greater (P < 0.05) than those of DDGS. The AID and SID values of Lys and a few other AA were similar in LCS (SID Lys: 63.1%) and DDGS (SID Lys: 61.5%), but the digestibility values of most AA in LCS were less than in DDGS (P < 0.05). The low digestibility of AA in LCS was most pronounced for Met (SID: LCS, 41.9% vs. DDGS, 82.8%). The LCS had less (P < 0.05) AID and SID of CP (SID: 67.8%) than intact SB (SID: 85.2%) and ground SB (SID: 85.9%) as well as all AA. The PDTS generally had the least AID and SID and had less (P < 0.05) CP (SID: 55.3%) and several AA, including Lys, compared with LCS. In conclusion, the presence of SB does not decrease AA digestibility of DDGS, and the LCS

  10. Sap-Sugar Content of Grafted Sugar Maple Trees

    Science.gov (United States)

    Maurice E. Jr. Demeritt; Maurice E. Jr. Demeritt

    1985-01-01

    In March and April 1983, 289 and 196 young grafted sugar maple trees were tapped and evaluated for sap-sugar content. In April, sap was collected from taps both above and below the graft union. Diameter of all tapped trees at 18 inches above the ground was measured. Analysis of the data revealed that: (1) trees selected for high sugar yield cannot be reproduced by...

  11. Changes in sugar content and related enzyme activities in table grape (Vitis vinifera L.) in response to foliar selenium fertilizer.

    Science.gov (United States)

    Zhu, Shuaimeng; Liang, Yinli; An, Xiaojuan; Kong, Fanchao; Gao, Dekai; Yin, Hongfei

    2017-09-01

    Spraying selenium (Se) fertilizer is an effective method for Se-enriched fruit production. Sugar content in fruit is the major factor determining berry quality. However, changes in sugar metabolism in response to Se fertilizer are unclear. Hence, this study was conducted to identify the effects of Se fertilizer on sugar metabolism and related enzyme activities of grape berries. Additionally, production of leaves with and without Se fertilizer was also investigated. Acid invertase (AI) activity, total soluble sugar and Se content in berries, and photosynthetic rate in leaves produced under Se fertilizer treatments were higher than that of control. Glucose and fructose were the primary sugars in berries, with a trace of sucrose. In both berries and leaves, neutral invertase activity was lower than AI, there was no significant difference in neutral invertase, sucrose synthase and sucrose phosphate synthase between Se fertilizer-treated and control. In berries, AI showed a significant positive correlation with glucose and fructose; also Se content was significantly correlated with sugar content. AI played an important role in the process of sugar accumulation in berries; high AI activity in berries and photosynthetic rate in leaves could explain the mechanism by which Se fertilizer affected sugar accumulation in berries. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  12. Agronomical Parameters, Sugar Profile and Antioxidant Compounds of “Catherine” Peach Cultivar Influenced by Different Plum Rootstocks

    Directory of Open Access Journals (Sweden)

    Carolina Font i Forcada

    2014-02-01

    Full Text Available The influence of seven plum rootstocks (Adesoto, Monpol, Montizo, Puebla de Soto 67 AD, PM 105 AD, St. Julien GF 655/2 and Constantí 1 on individual and total sugars, as well as on antioxidant content in fruit flesh of “Catherine” peaches, was evaluated for three years. Agronomical and basic fruit quality parameters were also determined. At twelve years after budding, significant differences were found between rootstocks for the different agronomic and fruit quality traits evaluated. The Pollizo plum rootstocks Adesoto and PM 105 AD seem to induce higher sweetness to peach fruits, based on soluble solids content, individual (sucrose, fructose and sorbitol and total sugars. A clear tendency was also observed with the rootstock Adesoto, inducing the highest content of phenolics, flavonoids, vitamin C and relative antioxidant capacity (RAC. Thus, the results of this study demonstrate the significant effect of rootstock on the sugar profile and phytochemical characteristics of peach fruits. In addition, this work shows the importance of the sugar profile, because specific sugars play an important role in peach flavour quality, as well as the studied phytochemical compounds when looking for high quality peaches with enhanced health properties.

  13. Solubility of carbohydrates in heavy water.

    Science.gov (United States)

    Cardoso, Marcus V C; Carvalho, Larissa V C; Sabadini, Edvaldo

    2012-05-15

    The solubility of several mono-(glucose and xylose), di-(sucrose and maltose), tri-(raffinose) and cyclic (α-cyclodextrin) saccharides in H(2)O and in D(2)O were measured over a range of temperatures. The solution enthalpies for the different carbohydrates in the two solvents were determined using the vant' Hoff equation and the values in D(2)O are presented here for the first time. Our findings indicate that the replacement of H(2)O by D(2)O remarkably decreases the solubilities of the less soluble carbohydrates, such as maltose, raffinose and α-cyclodextrin. On the other hand, the more soluble saccharides, glucose, xylose, and sucrose, are practically insensitive to the H/D replacement in water. Copyright © 2012 Elsevier Ltd. All rights reserved.

  14. Characterization of Physical and Mechanical Properties of Miscible Lactose-Sugars Systems.

    Science.gov (United States)

    Li, Runjing; Roos, Yrjö H; Miao, Song

    2017-09-01

    Lactose-sugars systems were produced by spray drying. They were lactose, lactose-glucose (4:1) mixtures, lactose-maltose (4:1) mixtures, lactose-sucrose (4:1) mixtures, lactose-trehalose (4:1) mixtures, and lactose-corn syrup solids (CSS) (4:1) mixtures. The physical characteristics, water sorption behavior, glass transition, and mechanical properties of miscible lactose-sugars systems were investigated. Lactose-glucose mixtures had larger particle size than other lactose-sugars systems after spray drying. The presence of glucose or sucrose in lactose-sugars mixtures decreased the glass transition temperatures of amorphous systems, while the presence of maltose and trehalose had only minor impact on the glass transition temperatures. Moreover, glucose accelerated the crystallization of amorphous system at 0.44 a w , but its presence delayed the loss of sorbed water at higher water activities (≥0.54 a w ). Mechanical property study indicated that glucose and sucrose in amorphous system could result in an increase of molecular mobility, while the presence of CSS could decrease the free volume and maintain the stiffness of the miscible systems. © 2017 Institute of Food Technologists®.

  15. Effect of amides on lithium tetraborate solubility

    Energy Technology Data Exchange (ETDEWEB)

    Tsekhanskij, R S; Skvortsov, V C; Molodkin, A K; Sadetdi-pov, Sh V [Chuvashskij Gosudarstvennyj Pedagogicheskij Inst., Cheboksary (USSR); Universitet Druzhby Narodov, Moscow (USSR))

    1983-03-01

    Using the methods of solubility, densi- and refractometry at 25 deg C, it has been established that the systems lithium tetraborate-formamide (acetamide, dimethyl-formamide)-water are of a simple eutonic type. Amides decrease the salt solubility. Lyotropic effect, as calculated for molar concentrations (-Lsub(M)) relative to the absolute value, increases from formamide to dimethyl-formamide. The sequence is determined by the fact that, when there is one or two hydrophilic methyl groups in amide molecules which are in contact with tetraborate, they decrease the hydration energy of lithium cations.

  16. Effect of amides on lithium tetraborate solubility

    International Nuclear Information System (INIS)

    Tsekhanskij, R.S.; Skvortsov, V.C.; Molodkin, A.K.; Sadetdi- pov, Sh.V.

    1983-01-01

    Using the methods of solubility, densi- and refractometry at 25 deg C, it has been established that the systemS lithium tetraborate-formamide (acetamide, dimethyl-formamide)-water are of a simple eutonic type. Amides decrease the salt solubility. Lyotropic effect, as calculated for molar concentrations (-Lsub(M)) relative to the absolute value, increases from formamide to dimethylformamide. The sequence is determined by the fact that, when there is one or two hydrophilic methyl groups in amide molecules which are in contact with tetraborate, they decrease the hydration energy of lithium cations

  17. Study of solubility of some metal cyclohexane carbonates

    International Nuclear Information System (INIS)

    Niyazov, A.N.; Amanov, K.B.; Trapeznikova, V.F.; Kul'maksimov, A.; Kolosova, N.

    1978-01-01

    The solubility of calcium, magnesium, strontium, barium, cabalt, copper and aluminium cyclohexane, carbonates (CHC) in water has been studied at 25 deg C. The salt solubility has been calculated according to the metal ion concentration in saturated solutions. It has been established, that the cobalt and rare earth cyclohexane carbonates are relatively very soluble in water and have solubility products of SP > 1x10 -5 . The solubility of CHC of multivalent metals increases with the decrease of pH values. Each salt has some ''limiting'' pH value of a solution, below which it decomposes completely and can not exist in a solution in the form of solid phase

  18. Sugar beet breeding

    Science.gov (United States)

    Sugar beet is a recent crop developed solely for extraction of the sweetener sucrose. Breeding and improvement of Beta vulgaris for sugar has a rich historical record. Sugar beet originated from fodder beet in the 1800s, and selection has increased sugar content from 4 to 6% then to over 18% today. ...

  19. Study of the properties of inverted liquid sugar processed with gamma radiation and electron beam

    International Nuclear Information System (INIS)

    Podadera, Priscilla

    2007-01-01

    Brazil occupies position of prominence in the sugar market and high consumption of this ingredient in the liquid form, in special for the beverages and food industries. The liquid sugar presents advantages in relation to the bulk granulated system such as easiness of the handling and dosage, reduced space for the storage, reduction of the losses, costs and hand of workmanship, improvement of the sanitizing and great possible variation in the ratios of different mixtures of sugars. The inverted liquid sugar is a solution of sucrose, glucose and fructose in water. This ingredient receives this name because during its production it has an inversion of the optic power of rotation of the solution. In the productive process of the inverted sugar some points occur that can generate contamination by microorganisms, so its become important the development of techniques that propitiate the efficient sanitizing of this raw material. This work aims at to consider two alternative techniques of microbiologic control: gamma radiation with source of cobalto-60 and radiation with electron beam. The study was lead in the doses of 5, 10, 20, 30 and 50 kGy. Concentration of sucrose presented in syrup decreased with the radiation indicating the scission of glycosidic linkage in addition with the glucose and fructose formation, generating the increase in solids soluble. The acid compounds formation occurred, confirmed by the reduction of pH, that it was proportional with the increase of the radiation dose. The biggest doses had provoked change of color in the syrup by the long molecular chain polymers formation. Viscosity in the samples processed by gamma radiation increased in relation to the control, indicating the prevalence of polymerization of molecules, whereas in the radiation with electron beam the polymerization was only observed in the sample that received the dose of 50 kGy, In the sensorial analysis, the judges had pointed significant difference (5%) in the flavor among

  20. Are sugar-free confections really beneficial for dental health?

    Science.gov (United States)

    Nadimi, H; Wesamaa, H; Janket, S-J; Bollu, P; Meurman, J H

    2011-10-07

    Various sugar substitutes have been introduced and are widely used in confections and beverages to avoid tooth decay from sugar and other fermentable carbohydrates. One group of sugar substitutes are sugar alcohols or polyols. They have been specifically used in foods for diabetic patients because polyols are not readily absorbed in the intestine and blood stream, preventing post-prandial elevation of glucose level. Additionally they may lower caloric intake. We searched PubMed, Cochrane Controlled Trials Registry, Cochrane Oral Health Review, Centre for Reviews and Dissemination in the UK, National Library for Public Health and a Centre for Evidence Based Dentistry website up to the end of October 2010, using the search terms 'sugar alcohol' or 'sugar-free' or 'polyols' and combined with a search with terms 'dental caries' or 'dental erosion'. Xylitol, a polyol, has been approved by the US Food and Drug Administration for its non-cariogenic properties that actually reduce the risk of dental decay and recently, the European Union also officially approved a health claim about xylitol as a 'tooth friendly' component in chewing gums. Although the presence of acidic flavourings and preservatives in sugar-free products has received less attention, these additives may have adverse dental health effects, such as dental erosion. Furthermore, the term sugar-free may generate false security because people may automatically believe that sugar-free products are safe on teeth. We concluded that polyol-based sugar-free products may decrease dental caries incidence but they may bring another dental health risk, dental erosion, if they contain acidic flavouring. There is a need for properly conducted clinical studies in this area.

  1. Improving the process of progressive preliming in sugar production

    Directory of Open Access Journals (Sweden)

    V. A. Golybin

    2016-01-01

    Full Text Available Tasks progressive preliming are to conduct neutralization, coagulation, deposition of non-sugars, the formation of a precipitate structure. This is the first operation of physical and chemical cleaning of the raw juice in the case of it in optimum conditions enables the coagulation and transferred to precipitate a substantial portion of high-molecular non-sugars, poorly soluble calcium salts that provides the cleaning effect of 14–18%, that is, up to half of the total effect on the whole station lime-carbon dioxide purification. The results preliming felt not only on the properties of the filtration of the carbonated juices, but a lso on the quality of performance of all intermediates and produced of sugar, in particular the color and turbidity of an aqueous solution, the content of the ash. In our investigations using the raw juice from sugar beet low quality found that when a uniform progressi ve change in pH of the juice during warm preliming best results for deposition rate and volume solids of the resulting precipitate are achieved when the length of 7 to 10 minutes. If it increases to 15 minutes and further reduced the deposition rate of the solid phase with a significant increase in turbidity decantate. It found that as a result of excess visit a large part of the raw juice in a progressive preliming at 60 °C increase in color prelimed juice was 30–55%, which is in agreement with the calculated values decay reducing agents in alkaline solution and formation of any additional colorants. The results confirming the feasibility of the use preddefekatsionnoy for processing the raw juice of condensed carbonate refunds.

  2. Changes in content and composition of sugar in molasses caused by gamma-irradiation

    International Nuclear Information System (INIS)

    Watanabe, Hiroshi; Sato, Tomotaro

    1980-01-01

    The sugar contents in undiluted molasses were found to be stable on γ-irradiation. The contents of total sugar and direct reducing sugar in cane molasses were completely unaffected by irradiation with doses of less than 3 Mrad. The reduction of total sugar content in beet molasses was 2% at 3 Mrad. On the other hand, in the molasses diluted with water, sugar contents decreased with the dose. Sucrose in molasses was degraded by irradiation, and subsequently glucose and fructose were formed. Raffinose in beet molasses was also degraded by irradiation, but galactose could not be detected. The irradiation temperature ranging from room temperature to 61 0 C, was hardly responsible for the change of sugar contents, but the changes in sugar caused by irradiation were enhanced at pHs of less than 4 and with an excess of oxygen. (author)

  3. Changes in content and composition of sugar in molasses caused by gamma-irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Watanabe, H; Sato, T

    1980-01-01

    The sugar contents in undiluted molasses were found to be stable on ..gamma..-irradiation. The contents of total sugar and direct reducing sugar in cane molasses were completely unaffected by irradiation with doses of less than 3 Mrad. The reduction of total sugar content in beet molasses was 2% at 3 Mrad. On the other hand, in the molasses diluted with water, sugar contents decreased with the dose. Sucrose in molasses was degraded by irradiation, and subsequently glucose and fructose were formed. Raffinose in beet molasses was also degraded by irradiation, but galactose could not be detected. The irradiation temperature ranging from room temperature to 61/sup 0/C, was hardly responsible for the change of sugar contents, but the changes in sugar caused by irradiation were enhanced at pHs of less than 4 and with an excess of oxygen.

  4. Changes in content and composition of sugar in molasses caused by gamma-irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Watanabe, H; Sato, T [Japan Atomic Energy Research Inst., Takasaki, Gunma. Takasaki Radiation Chemistry Research Establishment

    1980-08-01

    The sugar contents in undiluted molasses were found to be stable on ..gamma..-irradiation. The contents of total sugar and direct reducing sugar in cane molasses were completely unaffected by irradiation with doses of less than 3 Mrad. The reduction of total sugar content in beet molasses was 2% at 3 Mrad. On the other hand, in the molasses diluted with water, sugar contents decreased with the dose. Sucrose in molasses was degraded by irradiation, and subsequently glucose and fructose were formed. Raffinose in beet molasses was also degraded by irradiation, but galactose could not be detected. The irradiation temperature ranging from room temperature to 61/sup 0/C, was hardly responsible for the change of sugar contents, but the changes in sugar caused by irradiation were enhanced at pHs of less than 4 and with an excess of oxygen.

  5. Feasibility study of SMART core with soluble boron

    International Nuclear Information System (INIS)

    Kim, Kang Seog; Lee, Chung Chan; Zee, Sung Quun

    2000-11-01

    The excess reactivity of SMART core without soluble boron is effectively controlled by 49 CEDM. We suggest another method to control the core excess reactivity using both the checkerboard type of 25 CEDM and soluble boron and perform a feasibility calculation. The soluble boron operation is categorized into the on-line and the off-line mechanisms. The former is to successively control the boron concentration according to the excess reactivity during operation and the latter is to add and change some soluble boron during refueling and repairing. Since the on-line soluble boron control system of SMART is conceptually identical to that of the commercial pressurized water reactor, we did not perform the analysis. Since the soluble boron in the complete off-line system increases the moderator temperature coefficient, the reactivity defect between hot and cold moderator temperature is decreased. However, the decrease of the reactivity is not big to satisfy the core reactivity limits. When using 25 CEDM, the possible mechanism is to control the excess reactivity by both control rod and on-line boron control mechanism between cold and hot zero power and by only control rod at hot full power. We selected the loading pattern satisfying the requirement in the view of nuclear design

  6. Sustainability Issues and Opportunities in the Sugar and Sugar-Bioproduct Industries

    Directory of Open Access Journals (Sweden)

    Gillian Eggleston

    2015-09-01

    Full Text Available Like many other industries, the sugar and sugar-bioproduct industries are facing important sustainability issues and opportunities. The relatively low and fluctuating profit for sugar, surpluses of sugar, world-wide trend to produce alternative, renewable bio-based fuels and chemicals to those derived from petroleum and reduce greenhouse gases, water- and energy-intensive factories and refineries, and increased consumer demands for sustainably manufactured products are putting pressure on the industries to diversify for sustainability. Sugar crops, including sugar and energy cane (Saccharum officinarum, sugar and energy beets (Beta vulgaris, and sweet sorghum (Sorghum bicolor L. Moench, are excellent, renewable biomass feedstocks because of their availability, their being amongst the plants that give the highest yields of carbohydrates per hectare, and high sugar contents. While much research has been focused on conversion technologies for advanced biofuels and bioproducts, attention is now focused on developing sustainable supply chains of sugar feedstocks for the new, flexible biorefineries, with customers wanting maximum feedstock reliability and quality, while minimizing cost. All biomass from sugar crops are potential feedstocks. The cogeneration of bioelectricity from bagasse and leaf residues is being increasingly manufactured in more countries and, due to the high carbon content of bagasse and leaves, can also be converted into value-added products such as biochar. Sugar crops are superior feedstocks for the production of platform chemicals for the manufacture of a range of end-products, e.g., bioplastics, chemicals, and biomaterials. In several countries and regions, green sustainability criteria are now in place and have to be met to count against national biofuel targets. Processes to convert high-fiber sugar crop biomass into biofuel have been developed but there has only been limited commercialization at the large-scale.

  7. Role of potassium and nitrogen on sugar concentration of sugar beet

    African Journals Online (AJOL)

    Sugar is obtained from root of sugar beet (Beta vulgaris L.) in addition to other sources. Three important economic parameters are often considered and these are root yield, sugar concentration in root juice and total sugar yield. All the three are affected by cropping period and use of fertilisers. Existing literature suggests the ...

  8. Sensory and Physical Effects of Sugar Reduction in a Caramel Coating System.

    Science.gov (United States)

    Mayhew, Emily J; Schmidt, Shelly J; Lee, Soo-Yeun

    2017-08-01

    Sugar reduction in processed foods is a pressing and complex problem, as sugars contribute important sensory and physical properties to foods. Composed of sugars and lipids, caramel coating systems, like the coating in caramel popcorns, exemplify this challenge. In order to probe the feasibility and consequences of sugar reduction, both sensory and physical properties were measured for 3 types of caramel coating systems. Four commonly used sugar alcohols, isomalt, maltitol, mannitol, and sorbitol, with different thermal properties and relative sweetness values were chosen to replace sugar in the caramel coating systems at 25% and 50% sugar reduction levels. Full sugar (control) and reduced sugar caramel coating samples were prepared in duplicate. Ten trained panelists participated in a 6-wk descriptive analysis panel to define and quantify the intensity of important sensory characteristics. All 24 sensory terms generated by the panel differed significantly across caramel type and sugar replacer. Thermal properties were measured through differential scanning calorimetry, and textural properties were measured through texture profile analysis. Replacement of sugar with sugar alcohols was found to decrease the glass transition temperature and systematically alter the hardness and resilience of caramel samples. Principal component analysis of sensory and physical data revealed that caramel coating type dictates caramel aroma, aroma by mouth, taste, and aftertaste, while sugar replacer and replacement level dictate texture. This research represents the first comprehensive study of the effects of sugar reduction in a caramel coating system and suggests successful strategies for sugar reduction and key parameters to control in reduced sugar systems. © 2017 Institute of Food Technologists®.

  9. Effect of high-sugar grasses on methane emissions simulated using a dynamic model

    NARCIS (Netherlands)

    St-Pierre, J.L.; Dijkstra, J.; France, J.; Parsons, A.J.; Edwards, G.R.; Rasmussen, S.; Kebreab, E.; Bannink, A.

    2012-01-01

    High-sugar grass varieties have received considerable attention for their potential ability to decrease N excretion in cattle. However, feeding high-sugar grasses alters the pattern of rumen fermentation, and no in vivo studies to date have examined this strategy with respect to another

  10. Managing your blood sugar

    Science.gov (United States)

    Hyperglycemia - control; Hypoglycemia - control; Diabetes - blood sugar control; Blood glucose - managing ... sugar ( hypoglycemia ) Recognize and treat high blood sugar ( hyperglycemia ) Plan healthy meals Monitor your blood sugar (glucose) ...

  11. Effect of sorbitol on dough rheology and quality of sugar replaced cookies

    Directory of Open Access Journals (Sweden)

    Mohammad Ali Shariati

    2018-02-01

    Full Text Available A high amount of sugar is used in bakery products, which may cause diabetes, high blood glucose levels and obesity. Due to these reasons, sugar is being replaced with substitutes. There is different carbohydrate-based sugar substitutes (polyols that can efficiently replace sugar. Among polyols, sorbitol is an efficient replacer that can mimic sugar with minimal effects on cookie quality. Effects of different sorbitol levels (0 to 12.5% were seen on the dough rheology. Mixographic studies showed that peak height and mixing time reduced with the addition of sorbitol. Farinographic studies showed that water absorption and the mixing tolerance index of dough reduced with the supplementation of sorbitol, whereas dough development time, arrival time, dough stability time and softening of dough increased. Extensographic studies revealed that sorbitol substitution produced hard, cohesive, adhesive and elastic dough. Sugar in cookies formulations was reduced from 100 to 50% by replacing with sorbitol 0 to 50%. Physical analysis of sorbitol containing cookies showed that the diameter and spread factor of cookies decreased with higher levels of sorbitol, whereas thickness, color, hardness and water activity of cookies increased. The calorific value of cookies decreased with the increasing levels of sorbitol. At upto 20% replacement of sugar, other parameters of cookies were not affected. Sensory evaluation of the cookies showed that hedonic points for sensory evaluation parameters reduced with the increasing levels of sorbitol, T2 (20% replacement showed maximum overall acceptability. Normal 0 false false false EN-GB X-NONE AR-SA

  12. Intrinsic nitric oxide regulates the taste response of the sugar receptor cell in the blowfly, Phormia regina.

    Science.gov (United States)

    Murata, Yoshihiro; Mashiko, Masashi; Ozaki, Mamiko; Amakawa, Taisaku; Nakamura, Tadashi

    2004-01-01

    The taste organ in insects is a hair-shaped taste sensory unit having four functionally differentiated contact chemoreceptor cells. In the blowfly, Phormia regina, cGMP has been suggested to be a second messenger for the sugar receptor cell. Generally, cGMP is produced by membranous or soluble guanylyl cyclase (sGC), which can be activated by nitric oxide (NO). In the present paper, we electrophysiologically showed that an NO scavenger, 2-phenyl-4,4,5,5-tetramethylimidazoline-3-oxide-1-oxyl (PTIO), an NO donor, 1-hydroxy-2-oxo-3-(N-methyl-3-aminopropyl)-3-methyl-1-triazene (NOC 7) or an NO synthase (NOS) inhibitor, NG-nitro-L-arginine methyl ester (L-NAME) specifically affected the response in the sugar receptor cell, but not in other receptor cells. PTIO, when introduced into the receptor cells in a sensillum aided by sodium deoxycholate (DOC, pH 7.2), depressed the response of sugar receptor cells to sucrose but did not affect those of the salt or water receptor cells. NOC 7, given extracellularly, latently induced the response of sugar receptor cells; and L-NAME, when introduced into the receptor cells, depressed the response of sugar receptor cells. The results clearly suggest that NO, which may be produced by intrinsic NOS in sugar receptor cells, participates in the transduction cascade of these cells in blowfly.

  13. Identification, cloning and characterization of sis7 and sis10 sugar-insensitive mutants of Arabidopsis

    Directory of Open Access Journals (Sweden)

    Biddle Kelly D

    2008-10-01

    Full Text Available Abstract Background The levels of soluble sugars, such as glucose and sucrose, help regulate many plant metabolic, physiological and developmental processes. Genetic screens are helping identify some of the loci involved in plant sugar response and reveal extensive cross-talk between sugar and phytohormone response pathways. Results A forward genetic screen was performed to identify mutants with increased resistance to the inhibitory effects of high levels of exogenous sugars on early Arabidopsis seedling development. The positional cloning and characterization of two of these sugar insensitive (sis mutants, both of which are also involved in abscisic acid (ABA biosynthesis or response, are reported. Plants carrying mutations in SIS7/NCED3/STO1 or SIS10/ABI3 are resistant to the inhibitory effects of high levels of exogenous Glc and Suc. Quantitative RT-PCR analyses indicate transcriptional upregulation of ABA biosynthesis genes by high concentrations of Glc in wild-type germinating seeds. Gene expression profiling revealed that a significant number of genes that are expressed at lower levels in germinating sis7-1/nced3-4/sto1-4 seeds than in wild-type seeds are implicated in auxin biosynthesis or transport, suggesting cross-talk between ABA and auxin response pathways. The degree of sugar insensitivity of different sis10/abi3 mutant seedlings shows a strong positive correlation with their level of ABA insensitivity during seed germination. Conclusion Mutations in the SIS7/NCED3/STO1 gene, which is primarily required for ABA biosynthesis under drought conditions, confer a sugar-insensitive phenotype, indicating that a constitutive role in ABA biosynthesis is not necessary to confer sugar insensitivity. Findings presented here clearly demonstrate that mutations in ABI3 can confer a sugar-insensitive phenotype and help explain previous, mixed reports on this topic by showing that ABA and sugar insensitivity exhibit a strong positive correlation in

  14. Informatic System for Improve of Sugarcane Harvest in Cubans Sugar Factories

    Directory of Open Access Journals (Sweden)

    Alejandro Álvarez-Navarro

    2015-12-01

    Full Text Available The restructure of the sugar industry, led to the implementation of efficient methods in order to enhance the sugar cane harvest in the agroindustrial process. The Information and Communications Technology (ICT, associated to optimization methods, may allow take the right decisions at different levels of the productive process. The organization of the sugar cane harvest is an important moment within this scheme. The objective of this  investigation is to determine the optimal cutting strategy considering the new changes existing that permit obtain higher amount of sugar using the same quantity of raw material. Has been implemented a new informatic system, supported by a new branch of the goal programming with lexicographic priorities. The experimental results show that there is an impact at the planning level, as the cutting out of date decreases until a 15 %, and the sugar production increases in 2 043,89 t. 

  15. Effects of Mg on C and N Metabolism of Soybean at Different Nitrogen Supplying Levels

    Institute of Scientific and Technical Information of China (English)

    LANG Man; LIU Yuanying; PENG Xianlong; ZHANG Wenzhao

    2006-01-01

    A pot experiment was conducted to study the effects of magnesium on carbon and nitrogen metabolism of soybean at different nitrogen supplying levels. The results showed that the effects of magnesium at low nitrogen rate on N content, soluble protein, soluble sugar contents were not alike at different growth stage, although nodule dry weights raised, the yield and protein content of seeds decreased, however, the oil content was improved.The application of magnesium at medium and high nitrogen supplying levels promoted the uptake of N effectively,increased the soluble protein and soluble sugar contents, but the nodule dry weights of application magnesium at medium nitrogen supplying level decreased and the yield increased only a little despite the improved quality.Application of magnesium at high nitrogen supplying level raised nodule dry weights and soybean yield significantly, the quality of seeds was also improved.

  16. MICROFLORA OF BEET SUGAR PRODUCTION: PROBLEMS AND SOLUTIONS

    Directory of Open Access Journals (Sweden)

    N. G. Kulneva

    2014-01-01

    Full Text Available Summary. Sugar beet is one of the strategic crops for food safety of Russia. The lack of specialized warehouse for harvest does not provide storage of roots for a long time. In the case of a thaw roots that have been defrosted unsuitable for processing. Beet and products of its processing is a good object for the development of microorganisms. Permanent microflora of sugar production are: Bacillus subtilis, Clostridium perfringes, Leuconostoc dextranicum, Torula alba, Pseudomonas fluorescens, Sarcina lutea and other kinds of microorganisms, leading to a problem processing of beet root and reduced quality of sugar. The most dangerous is the slimy bacteriosis is a bacterial disease beet caused by heterofermentative cocci of Leuconostoc (Leuconostoc mesenteroides, L. dextranicum. Product of the vital activity of microorganisms is dextran, which is synthesized from sucrose as a result of dextrany or mucous fermentation and leads to significant technological problems in processing of infected beet. Improving the efficiency of sugar production is connected with decrease in loss of quality of raw material preparation, storing and processing of sugar beet. At sugar plants use a variety of drugs that suppress the growth of pathogenic microflora, but there comes a rapid adaptation of microorganisms, therefore there is a need to implement new products to prevent damage to roots and improve the quality of produced sugar. To resolve this problem experimentally selected bactericidal drug, defined its rational concentration and conditions for the use in sugar beet production. The use of antibacterial drug in the process of extraction allows to increase the purity of diffusion juice 1.3 %, reduce the protein content in it (12.5 %; with the purity of the pure juice increases by 1.1 %, its color index is reduced by 44.7 %.

  17. Taste-independent detection of the caloric content of sugar in Drosophila.

    Science.gov (United States)

    Dus, Monica; Min, SooHong; Keene, Alex C; Lee, Ga Young; Suh, Greg S B

    2011-07-12

    Feeding behavior is influenced primarily by two factors: nutritional needs and food palatability. However, the role of food deprivation and metabolic needs in the selection of appropriate food is poorly understood. Here, we show that the fruit fly, Drosophila melanogaster, selects calorie-rich foods following prolonged food deprivation in the absence of taste-receptor signaling. Flies mutant for the sugar receptors Gr5a and Gr64a cannot detect the taste of sugar, but still consumed sugar over plain agar after 15 h of starvation. Similarly, pox-neuro mutants that are insensitive to the taste of sugar preferentially consumed sugar over plain agar upon starvation. Moreover, when given a choice between metabolizable sugar (sucrose or D-glucose) and nonmetabolizable (zero-calorie) sugar (sucralose or L-glucose), starved Gr5a; Gr64a double mutants preferred metabolizable sugars. These findings suggest the existence of a taste-independent metabolic sensor that functions in food selection. The preference for calorie-rich food correlates with a decrease in the two main hemolymph sugars, trehalose and glucose, and in glycogen stores, indicating that this sensor is triggered when the internal energy sources are depleted. Thus, the need to replenish depleted energy stores during periods of starvation may be met through the activity of a taste-independent metabolic sensing pathway.

  18. SNPs in genes functional in starch-sugar interconversion associate with natural variation of tuber starch and sugar content of potato (Solanum tuberosum L.).

    Science.gov (United States)

    Schreiber, Lena; Nader-Nieto, Anna Camila; Schönhals, Elske Maria; Walkemeier, Birgit; Gebhardt, Christiane

    2014-07-31

    Starch accumulation and breakdown are vital processes in plant storage organs such as seeds, roots, and tubers. In tubers of potato (Solanum tuberosum L.) a small fraction of starch is converted into the reducing sugars glucose and fructose. Reducing sugars accumulate in response to cold temperatures. Even small quantities of reducing sugars affect negatively the quality of processed products such as chips and French fries. Tuber starch and sugar content are inversely correlated complex traits that are controlled by multiple genetic and environmental factors. Based on in silico annotation of the potato genome sequence, 123 loci are involved in starch-sugar interconversion, approximately half of which have been previously cloned and characterized. By means of candidate gene association mapping, we identified single-nucleotide polymorphisms (SNPs) in eight genes known to have key functions in starch-sugar interconversion, which were diagnostic for increased tuber starch and/or decreased sugar content and vice versa. Most positive or negative effects of SNPs on tuber-reducing sugar content were reproducible in two different collections of potato cultivars. The diagnostic SNP markers are useful for breeding applications. An allele of the plastidic starch phosphorylase PHO1a associated with increased tuber starch content was cloned as full-length cDNA and characterized. The PHO1a-HA allele has several amino acid changes, one of which is unique among all known starch/glycogen phosphorylases. This mutation might cause reduced enzyme activity due to impaired formation of the active dimers, thereby limiting starch breakdown. Copyright © 2014 Schreiber et al.

  19. Effects of sugar rich diet on brain serotonin, hyperphagia and anxiety in animal model of both genders.

    Science.gov (United States)

    Inam, Qurrat-ul-Aen; Ikram, Huma; Shireen, Erum; Haleem, Darakhshan Jabeen

    2016-05-01

    Lower levels of 5-hydroxytryptamine (5-HT; serotonin) in the brain elicit sugar craving, while ingestion of sugar rich diet improves mood and alleviates anxiety. Gender differences occur not only in brain serotonin metabolism but also in a serotonin mediated functional responses. The present study was therefore designed to investigate gender related differences on the effects of long term consumption of sugar rich diet on the metabolism of serotonin in the hypothalamus and whole brain which may be relevant with the hyperphagic and anxiety reducing effects of sugar rich diet. Male and female rats were fed freely on a sugar rich diet for five weeks. Hyperphagic effects were monitored by measuring total food intake and body weights changes during the intervention. Anxiolytic effects of sugar rich diet was monitored in light-dark transition test. The results show that ingestion of sugar rich diet decreased serotonin metabolism more in female than male rats. Anxiolytic effects were elicited only in male rats. Hyperphagia was comparable in both male and female rats. Finings would help in understanding the role of sugar rich diet-induced greater decreases of serotonin in sweet craving in women during stress.

  20. Effect of pre sowing gamma irradiation on yield and quality traits of sugar beet multi germ varieties

    International Nuclear Information System (INIS)

    Moustafa, R.A.; Elgeddawy, I.W.; Tolba, A.M.

    1994-01-01

    In this study which was carried out during two growing seasons in the experimental farm of the atomic energy authority, Egypt, seeds of the sugar beet varieties gloria and vital were subjected to different gamma irradiation doses from 0.0 to 1000 gray. Obtained results indicate that dosages up to 400 gray affected insignificantly on germination percent, while the higher doses had a progressive depression on the percentage of the germinated seeds of the both varieties. Stimulating effect of gamma irradiation on seeding height and seeding fresh weight was clearly shown following irradiating vital seeds with 100 or 200 gray, while marked reduction in mean values of these traits was noticed both varieties in the treatments of 500 gray or more. Considerable increases in root and leaves yield/plant of vital and gloria were detected after the application of 200 and 300 gray, respectively seeds received 500 gray or more gave plants with low productivity of roots and leaves. The chemical constituent of sugar beet roots was markedly influenced by gamma ray treatments, a dose of 400 gray existed a favourable and pronounced effect on sucrose content of the couple varieties, while the stimulating effects of gamma dosages on purity % of vital and gloria occurred at the higher doses of 500 and 750 gray. On the contrary, these dosages decreased remarkable the total soluble solids %. Based on the exhibited results, it could be concluded that performing gamma rays at dosages located between 200 and 400 gray may be of significant role in enhancing the quantitative and qualitative characteristics of sugar beet genotypes. 2 tabs

  1. Kinetics of ethanol production by immobilized Kluyveromyces marxianus cells at varying sugar concentrations of Jerusalem artichoke juice

    Energy Technology Data Exchange (ETDEWEB)

    Bajpai, P.; Margaritis, A.

    1987-08-01

    Kinetics of ethanol fermentation at varying sugar concentrations of Jerusalem artichoke tuber extract has been studied using Kluyveromyces marxianus cells immobilized in calcium alginate gel beads. A maximum ethanol concentration of 111 g/l was achieved at an initial sugar concentration of 260 g/l in 20 hours, when the immobilized cell concentration in the calcium alginate beads was 53.3 g dry wt./l bead volume. Ethanol yield remained almost unaffected by initial sugar concentration up to 250 g/l and was found to be about 88% of the theoretical. Maximum rate of ethanol production decreased from 22.5 g ethanol/l/h to 10.5 g ethanol/l/h while the maximum rate of total sugars utilization decreased from 74.9 g sugars/l/h to 28.5 g sugars/l/h as the initial substrate concentration was increased from 100 to 300 g/l. The concentration of free cells in the fermentation broth was low.

  2. Added sugars drive nutrient and energy deficit in obesity: a new paradigm.

    Science.gov (United States)

    DiNicolantonio, James J; Berger, Amy

    2016-01-01

    Obesity has traditionally been thought of as a state of caloric imbalance, where the intake of calories exceeds the expenditure or 'burning' of calories. However, a more nuanced appreciation for the complex biochemistry and physiology of cellular energy generation suggests that obesity is a state of hormonal imbalance causing increased shunting of food energy into adipose tissue for storage, resulting in decreased satiety and ultimately leading to increased caloric intake. Adding to this hypothesis, we propose that obesity is also a state of nutrient and energy deficit, leading to decreased fatty acid mobilisation and oxidation, the result of which may be a natural disinclination towards physical activity. Added sugars (sucrose, a.k.a. table sugar and high-fructose corn syrup) may provide energy (4 kcal/g) but at current intakes they do not facilitate-and may even hinder-the production of energy. Not only do added sugars displace nutritionally superior foods in the diet, but they may also deplete nutrients from other foods that have been consumed, as well as from body stores, in order to enable their proper oxidation and liberate their calories as energy. Additionally, the consumption of added sugars damages the mitochondria and hence impairs energy generation. Moreover, overconsuming added sugars may result in a kind of 'internal starvation' (via leptin and insulin resistance) leading to further hunger signals in the body. Added sugars promote nutrient and energy deficit and through this novel pathway promote obesity.

  3. High sugar diet disrupts gut homeostasis though JNK and STAT pathways in Drosophila.

    Science.gov (United States)

    Zhang, Xiaoyue; Jin, Qiuxia; Jin, Li Hua

    2017-06-10

    The incidence of diseases associated with a high sugar diet has increased in the past years, and numerous studies have focused on the effect of high sugar intake on obesity and metabolic syndrome. However, how a high sugar diet influences gut homeostasis is still poorly understood. In this study, we used Drosophila melanogaster as a model organism and supplemented a culture medium with 1 M sucrose to create a high sugar condition. Our results indicate that a high sugar diet promoted differentiation of intestinal stem cells through upregulation of the JNK pathway and downregulation of the JAK/STAT pathway. Moreover, the number of commensal bacteria decreased in the high sugar group. Our data suggests that the high caloric diet disrupts gut homeostasis and highlights Drosophila as an ideal model system to explore gastrointestinal disease. Copyright © 2017 Elsevier Inc. All rights reserved.

  4. Effect of ozonation on the reactivity of lignocellulose substrates in enzymatic hydrolyses to sugars

    Science.gov (United States)

    Ben'ko, E. M.; Manisova, O. R.; Lunin, V. V.

    2013-07-01

    The efficiency of pre-treatment of aspen wood with ozone for subsequent enzymatic hydrolysis into sugars is determined by the amount of absorbed ozone. The ozone absorption rate depended on the water content in the sample being ozonized and was maximum at a relative humidity of wood of ˜40%. As a result of ozone pre-treatment, the initial rate of the enzymatic hydrolysis of wood under the action of a cellulase complex increased eightfold, and the maximum yield of sugars increased tenfold depending on the ozone dose. The ozonation at ozone doses of more than 3 mol/PPU (phenylpropane structural unit of lignin) led to a decrease in the yield of sugars because of the oxidative destruction of cellulose and hemicellulose. The alkaline ozonation in 2 and 12% NaOH was inefficient because of the accompanying oxidation of carbohydrates and considerably decreased the yield of sugars.

  5. Polysaccharide components from the scape of Musa paradisiaca: main structural features of water-soluble polysaccharide component.

    Science.gov (United States)

    Anjaneyalu, Y V; Jagadish, R L; Raju, T S

    1997-06-01

    Polysaccharide components present in the pseudo-stem (scape) of M. paradisiaca were purified from acetone powder of the scape by delignification followed by extraction with aqueous solvents into water soluble polysaccharide (WSP), EDTA-soluble polysaccharide (EDTA-SP), alkali-soluble polysaccharide (ASP) and alkali-insoluble polysaccharide (AISP) fractions. Sugar compositional analysis showed that WSP and EDTA-SP contained only D-Glc whereas ASP contained D-Glc, L-Ara and D-Xyl in approximately 1:1:10 ratio, respectively, and AISP contained D-Glc, L-Ara and D-Xyl in approximately 10:1:2 ratio, respectively. WSP was further purified by complexation with iso-amylalcohol and characterized by specific rotation, IR spectroscopy, Iodine affinity, ferricyanide number, blue value, hydrolysis with alpha-amylase and glucoamylase, and methylation linkage analysis, and shown to be a amylopectin type alpha-D-glucan.

  6. Intraspecies differences in cold hardiness, carbohydrate content and β-amylase gene expression of Vaccinium corymbosum during cold acclimation and deacclimation.

    Science.gov (United States)

    Lee, Jun Hyung; Yu, Duk Jun; Kim, Su Jin; Choi, Doil; Lee, Hee Jae

    2012-12-01

    Changes in cold hardiness, carbohydrate content and β-amylase gene expression were monitored in the shoots of the highbush blueberry (Vaccinium corymbosum L.) cultivars 'Sharpblue' and 'Jersey' during cold acclimation (CA) and deacclimation (DA). The seasonal patterns were similar in both cultivars, but the levels of cold hardiness determined by electrolyte leakage analysis were significantly different; 'Jersey' was hardier than 'Sharpblue'. Cold hardiness was closely related to total soluble sugar content (r = -0.98** and -0.99** for 'Sharpblue' and 'Jersey', respectively). In 'Jersey', more soluble sugars accumulated during CA. Of the detected soluble sugars, glucose, fructose and raffinose contents were significantly associated with cold hardiness in both cultivars. Sucrose was abundant in both cultivars, and stachyose content changed significantly during CA and DA. However, they were not associated with cold hardiness. A sharp decrease in starch contents in the middle of CA coincided with β-amylase gene (VcBMY) expression, indicating the conversion of starch into soluble sugars. During CA, VcBMY was expressed up to twofold higher in 'Jersey' than in 'Sharpblue'. These results suggest that intraspecies differences in the cold hardiness of highbush blueberries are associated with total soluble sugar content, which is driven partly by differential expression of VcBMY.

  7. Effect of pulp preconditioning on acidification, proteolysis, sugars and free fatty acids concentration during fermentation of cocoa (Theobroma cacao) beans.

    Science.gov (United States)

    Afoakwa, Emmanuel Ohene; Quao, Jennifer; Budu, Agnes Simpson; Takrama, Jemmy; Saalia, Firibu Kwesi

    2011-11-01

    Changes in acidification, proteolysis, sugars and free fatty acids (FFAs) concentrations of Ghanaian cocoa beans as affected by pulp preconditioning (pod storage or PS) and fermentation were investigated. Non-volatile acidity, pH, proteolysis, sugars (total, reducing and non-reducing) and FFAs concentrations were analysed using standard methods. Increasing PS consistently decreased the non-volatile acidity with concomitant increase in pH during fermentation of the beans. Fermentation decreased the pH of the unstored beans from 6.7 to 4.9 within the first 4 days and then increased slightly again to 5.3 by the sixth day. Protein, total sugars and non-reducing sugars decreased significantly (p cocoa beans was largely affected by fermentation than by PS.

  8. Grapevine tissues and phenology differentially affect soluble carbohydrates determination by capillary electrophoresis.

    Science.gov (United States)

    Moreno, Daniela; Berli, Federico; Bottini, Rubén; Piccoli, Patricia N; Silva, María F

    2017-09-01

    Soluble carbohydrates distribution depends on plant physiology and, among other important factors, determines fruit yield and quality. In plant biology, the analysis of sugars is useful for many purposes, including metabolic studies. Capillary electrophoresis (CE) proved to be a powerful green separation technique with minimal sample preparation, even in complex plant tissues, that can provide high-resolution efficiency. Matrix effect refers to alterations in the analytical response caused by components of a sample other than the analyte of interest. Thus, the assessment and reduction of the matrix factor is fundamental for metabolic studies in different matrices. The present study evaluated the source and levels of matrix effects in the determination of most abundant sugars in grapevine tissues (mature and young leaves, berries and roots) at two phenological growth stages. Sucrose was the sugar that showed the least matrix effects, while fructose was the most affected analyte. Based on plant tissues, young leaves presented the smaller matrix effects, irrespectively of the phenology. These changes may be attributed to considerable differences at chemical composition of grapevine tissues with plant development. Therefore, matrix effect should be an important concern for plant metabolomics. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  9. Study of sugar phloem unloading in ripening grape berries under water stress conditions

    Directory of Open Access Journals (Sweden)

    Zenphing Wang

    2003-12-01

    Full Text Available Sugar phloem unloading in ripening grape berries (Vitis vinifera L. cv. Syrah was studied under water stress conditions using the «beny-cup» technique. After veraison, berry growth, the potential Exposed Leaf Area (pELA and photosynthetic activity are clearly reduced in water-stressed vines (- 0.5 > Ψb > - 0.6 MPa as compared to normal 1 Ψ-watcred vines (Mb = - 0.2 MPa. The ratio pELA/yield is also reduced, which is particular to this experiment. The beiries' ripening period (between veraison and maturity can be divided into three growth phases, Illa, Illb and IIlc. During phase Ma, the berries grow rapidly; at this point, water stress severely inhibits cell expan¬ sion of the berries but does not impact on daily sugar accumulation. During phase Mb, the berries grow slowly in both water-stressed and control vines. Water stress can shorten this phase and reduce sugar accumulation in the berries by decreasing daily sugar unloading. During phase II le, the Iresh weight and volume of the berries decreases as does the daily sugar unloading. During the day, sugar unloading in ripening berries occurs mainly in the morning (7 am to 10.30 am and at noon (1 to 1.30 pm; little sugar is unloaded in the afternoon (4 pm to 4.30 pin. Moderate water stress from veraison to maturity affects végétative growth (i.e. the growth of primary and secoridary shoots, and reduces the exposed leaf area, photosynthetic activity, berry growth, and the accumulation of sugar at the end of ripening (phases Mb and IIlc.

  10. Thermal, mechanical, and physical properties of seaweed/sugar palm fibre reinforced thermoplastic sugar palm Starch/Agar hybrid composites.

    Science.gov (United States)

    Jumaidin, Ridhwan; Sapuan, Salit M; Jawaid, Mohammad; Ishak, Mohamad R; Sahari, Japar

    2017-04-01

    The aim of this research is to investigate the effect of sugar palm fibre (SPF) on the mechanical, thermal and physical properties of seaweed/thermoplastic sugar palm starch agar (TPSA) composites. Hybridized seaweed/SPF filler at weight ratio of 25:75, 50:50 and 75:25 were prepared using TPSA as a matrix. Mechanical, thermal and physical properties of hybrid composites were carried out. Obtained results indicated that hybrid composites display improved tensile and flexural properties accompanied with lower impact resistance. The highest tensile (17.74MPa) and flexural strength (31.24MPa) was obtained from hybrid composite with 50:50 ratio of seaweed/SPF. Good fibre-matrix bonding was evident in the scanning electron microscopy (SEM) micrograph of the hybrid composites' tensile fracture. Fourier transform infrared spectroscopy (FT-IR) analysis showed increase in intermolecular hydrogen bonding following the addition of SPF. Thermal stability of hybrid composites was enhanced, indicated by a higher onset degradation temperature (259°C) for 25:75 seaweed/SPF composites than the individual seaweed composites (253°C). Water absorption, thickness swelling, water solubility, and soil burial tests showed higher water and biodegradation resistance of the hybrid composites. Overall, the hybridization of SPF with seaweed/TPSA composites enhances the properties of the biocomposites for short-life application; that is, disposable tray, plate, etc. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. The Truth about Sugar.

    Science.gov (United States)

    Yeung, C Albert; Goodfellow, Ashley; Flanagan, Louise

    2015-01-01

    Sugars are used by the industry to enhance the attractiveness of foods and drinks. These added sugars, or 'free sugars', are not easily identified in food or drink labels. Certain manufactured foods and drinks with 'safe' names, such as dried fruit and fruit juice, still contain free sugars and can be confusing. Guidance states that daily consumption of free sugars should be less than 10% of total energy intake (no more than 5% in the UK). However, it is found that both tooth decay and obesity are associated with consumption of free sugars in large quantities and at inappropriate times.

  12. Sugar-Sweetened Beverages Are the Main Sources of Added Sugar Intake in the Mexican Population.

    Science.gov (United States)

    Sánchez-Pimienta, Tania G; Batis, Carolina; Lutter, Chessa K; Rivera, Juan A

    2016-09-01

    Sugar intake has been associated with an increased prevalence of obesity, other noncommunicable diseases, and dental caries. The WHO recommends that free sugars should be ENSANUT (National Health and Nutrition Survey) 2012], which represents 3 geographic regions and urban and rural areas. Dietary information was obtained by administering a 24-h recall questionnaire to 10,096 participants. Total sugar intake was estimated by using the National Institute of Public Health (INSP) food-composition table and an established method to estimate added sugars. The mean intakes of total, intrinsic, and added sugars were 365, 127, and 238 kcal/d, respectively. Added sugars contributed 13% of TEI. Sugar-sweetened beverages (SSBs) were the main source of sugars, contributing 69% of added sugars. Food products high in saturated fat and/or added sugar (HSFAS) were the second main sources of added sugars, contributing 25% of added sugars. The average intake of added sugars in the Mexican diet is higher than WHO recommendations, which may partly explain the high prevalence of obesity and diabetes in Mexico. Because SSBs and HSFAS contribute >94% of total added sugars, strategies to reduce their intake should be strengthened. This includes stronger food labels to warn the consumer about the content of added sugars in foods and beverages. © 2016 American Society for Nutrition.

  13. Changes in physical, chemical and functional properties of whey protein isolate (WPI) and sugar beet pectin (SBP) conjugates formed by controlled dry-heating

    Science.gov (United States)

    A Maillard type reaction in the dry state was utilized to create conjugates between whey protein isolate (WPI) and sugar beet pectin (SBP) to achieve improved functional properties including solubility, colloidal stability and oil-in-water emulsion stability. To optimize the reaction conditions, mi...

  14. Effect of fasting on the urinary excretion of water-soluble vitamins in humans and rats.

    Science.gov (United States)

    Fukuwatari, Tsutomu; Yoshida, Erina; Takahashi, Kei; Shibata, Katsumi

    2010-01-01

    Recent studies showed that the urinary excretion of the water-soluble vitamins can be useful as a nutritional index. To determine how fasting affects urinary excretion of water-soluble vitamins, a human study and an animal experiment were conducted. In the human study, the 24-h urinary excretion of water-soluble vitamins in 12 healthy Japanese adults fasting for a day was measured. One-day fasting drastically decreased urinary thiamin content to 30%, and increased urinary riboflavin content by 3-fold. Other water-soluble vitamin contents did not show significant change by fasting. To further investigate the alterations of water-soluble vitamin status by starvation, rats were starved for 3 d, and water-soluble vitamin contents in the liver, blood and urine were measured during starvation. Urinary excretion of thiamin, riboflavin, vitamin B(6) metabolite 4-pyridoxic acid, nicotinamide metabolites and folate decreased during starvation, but that of vitamin B(12), pantothenic acid and biotin did not. As for blood vitamin levels, only blood vitamin B(1), plasma PLP and plasma folate levels decreased with starvation. All water-soluble vitamin contents in the liver decreased during starvation, whereas vitamin concentrations in the liver did not decrease. Starvation decreased only concentrations of vitamin B(12) and folate in the skeletal muscle. These results suggest that water-soluble vitamins were released from the liver, and supplied to the peripheral tissues to maintain vitamin nutrition. Our human study also suggested that the effect of fasting should be taken into consideration for subjects showing low urinary thiamin and high urinary riboflavin.

  15. 75 FR 60715 - Domestic Sugar Program-FY 2010 and FY 2011 Cane Sugar and Beet Sugar Marketing Allotments and...

    Science.gov (United States)

    2010-10-01

    ... marketing allotment and the associated production history will be transferred from MDFC to WSG, effective... Cane Sugar and Beet Sugar Marketing Allotments and Company Allocations AGENCY: Commodity Credit... publish the modifications to the fiscal year 2010 (FY 2010) State sugar marketing allotments and company...

  16. Flux, rejection and fouling during microfiltration and ultrafiltration of sugar palm sap using a pilot plant scale

    Directory of Open Access Journals (Sweden)

    Wanichapichart, P.

    2006-07-01

    Full Text Available The possibility of using a pilot plant scale microfiltration (MF and ultrafiltration (UF to clarify and reduce number of bacteria, yeast and mould of sugar palm sap was studied. The membrane used was multi channel tubular ceramic membrane (ZrO2-TiO2 with membrane pore size 0.2 and 0.1 μm and molecular weight cut off (MWCO 300 and 50 kDa for microfiltration and ultrafiltration respectively. The experiment was carried out to investigate the rejection of the components in sugar palm sap, permeate flux and fouling characteristics. The results showed that the turbidity, the total solid, the viscosity and the numbers of bacteria, yeast and mould in the permeate obtained by MF and UF were reduced significantly compared to those of fresh sugar palm sap. The total soluble solid, total sugar, reducing sugar and pH were not affected by MF and UF. The permeate fluxes for all membranes were reduced greatly as the volume concentration ratio (VCR increased due to severe fouling. The irreversible fouling on membrane surface and/or inside the membrane tended to increase with increasing membrane pore size or MWCO. The result also suggested that protein and small particle in the sugar palm sap were probably responsible for the internal fouling of large pore size membrane. According to the physical, chemical and microorganism quality results, both MF and UF showed the potential use for improving the quality of sugar palm sap but flux reduction due to fouling was a major problem affecting the process performance.

  17. Organic compounds in hot-water-soluble fractions from water repellent soils

    Science.gov (United States)

    Atanassova, Irena; Doerr, Stefan

    2014-05-01

    Water repellency (WR) is a soil property providing hydrophobic protection and preventing rapid microbial decomposition of organic matter entering the soil with litter or plant residues. Global warming can cause changes in WR, thus influencing water storage and plant productivity. Here we assess two different approaches for analysis of organic compounds composition in hot water extracts from accelerated solvent extraction (ASE) of water repellent soils. Extracts were lyophilized, fractionated on SiO2 (sand) and SPE cartridge, and measured by GC/MS. Dominant compounds were aromatic acids, short chain dicarboxylic acids (C4-C9), sugars, short chain fatty acids (C8-C18), and esters of stearic and palmitic acids. Polar compounds (mainly sugars) were adsorbed on applying SPE clean-up procedure, while esters were highly abundant. In addition to the removal of polar compounds, hydrophobic esters and hydrocarbons (alkanes and alkenes particle wettability and C dynamics in soils. Key words: soil water repellency, hot water soluble carbon (HWSC), GC/MS, hydrophobic compounds

  18. Serum Level of a Soluble Form of Endoglin (CD105 is Decreased after Goeckerman’s Therapy of Psoriasis

    Directory of Open Access Journals (Sweden)

    David Pohl

    2011-01-01

    Full Text Available Background. Goeckerman’s therapy (GT of psoriasis is based on daily application of pharmacy grade coal tar on affected skin with subsequent exposure to UV light. Disturbances in angiogenic activity are characteristic for the immunopathogenesis of psoriasis. The aim of study was to evaluate the influence of GT of psoriasis on proinflammatory and angiogenic activities expressed as changes in levels of endoglin (CD105. Methods. Serum levels of a soluble form of endoglin were measured in peripheral blood samples of 38 patients with psoriasis before and after therapy. Sixty three otherwise healthy blood donors serve as a control group. The efficacy of GT was expressed as changes in Psoriasis Area and Severity Index (PASI. Results. PASI score was significantly diminished by GT (p<0.001. Serum levels of soluble CD105 were significantly diminished after GT. The serum level of soluble CD105 dropped from 7.85 ± 2.26 ng/ml before therapy to 7.01 ± 1.71 ng/ml after therapy (p= 0.0002. Compared to serum levels of soluble CD105 in healthy blood donors, serum levels of soluble CD105 in patients before GT were significantly higher (p<0.001 and remained elevated after therapy (p<0.001. Angiogenic activity expressed as serum endoglin is diminished in patients with psoriasis treated by GT.

  19. Comparison of dynamic changes in endogenous hormone level, water content and water-soluble sugars in camellia oleifera of different ages

    International Nuclear Information System (INIS)

    Hu, J.; Shah, F.A.; Fu, S.; Wu, W.

    2017-01-01

    The aim of this study is to investigate and understand the dynamic changes and the possible functions of hormones, water and sugar content during the development of C. oleifera. We analyzed and compared WWC, WSS and five endogenous hormones (DHZR, ZR, GA, IAA and ABA) contents between May and August in C. oleifera plants at different ages from the same orchard. The results showed that WWC, WSS, DHZR, ZR, GA, IAA and ABA contents varied from 61.64%-74.15%, 21.24mg/g-62.44mg/g, 6.237ng/g-38.342ng/g, 6.215ng/g-16.721ng/g, 5.668ng/g-18.024ng/g, 24.753ng/g-147.507ng/g and 66.541ng/g-156.212ng/g, respectively. WWC showed a decrease-increase-decrease variation pattern, while the WSS contents demonstrated a generally increasing trend with increasing tree age. The levels of ABA in leaves sampled in August was higher than that in May, but the opposite was found for the GA and IAA levels. Tree ages were negatively correlated with ZR and GA concentration at the 1% or 5% significance level. The results of this study broaden our understanding of the interrelationships between phytohormones, WWC, and WSS content in the growth and development of C. oleifera. (author)

  20. In vitro dynamic solubility test: influence of various parameters.

    Science.gov (United States)

    Thélohan, S; de Meringo, A

    1994-10-01

    This article discusses the dissolution of mineral fibers in simulated physiological fluids (SPF), and the parameters that affect the solubility measurement in a dynamic test where an SPF runs through a cell containing fibers (Scholze and Conradt test). Solutions simulate either the extracellular fluid (pH 7.6) or the intracellular fluid (pH 4.5). The fibers have various chemical compositions and are either continuously drawn or processed as wool. The fiber solubility is determined by the amount of SiO2 (and occasionally other ions) released in the solution. Results are stated as percentage of the initial silica content released or as dissolution rate v in nm/day. The reproducibility of the test is higher with the less soluble fibers (10% solubility), than with highly soluble fibers (20% solubility). The influence of test parameters, including SPF, test duration, and surface area/volume (SA/V), has been studied. The pH and the inorganic buffer salts have a major influence: industrial glasswool composition is soluble at pH 7.6 but not at pH 4.5. The opposite is true for rock- (basalt) wool composition. For slightly soluble fibers, the dissolution rate v remains constant with time, whereas for highly soluble fibers, the dissolution rate decreases rapidly. The dissolution rates believed to occur are v1, initial dissolution rate, and v2, dissolution rate of the residual fibers. The SA of fibers varies with the mass of the fibers tested, or with the fiber diameter at equal mass. Volume, V, is the chosen flow rate. An increase in the SA/V ratio leads to a decrease in the dissolution rate.(ABSTRACT TRUNCATED AT 250 WORDS)

  1. Consumption of Added Sugars and Cardiometabolic Risk Indicators Among US Adolescents

    Science.gov (United States)

    Welsh, Jean A.; Sharma, Andrea; Argeseanu, Solveig; Vos, Miriam B.

    2014-01-01

    Background Increased carbohydrate and sugar consumption has been associated with dyslipidemia among adults. However, the effect of high consumption of added sugars (caloric sweeteners) on measures of cardiometabolic risk among US adolescents is unknown. Methods and Results This was a cross-sectional study of 2,252 US adolescents (13–18 y) in the National Health and Nutrition Examination Survey (NHANES) 1999–2004. Dietary data from one 24-hour recall were merged with added sugar content data from the USDA MyPyramid Equivalents Databases. Multivariate-adjusted means of cardiometabolic indicators were estimated by added sugar consumption level (added sugars was 21.4% of daily energy intake. Adjusted mean high-density lipoprotein cholesterol (HDL) levels were lower, 1.38 mmol/L (95% CI: 1.32, 1.43) among the lowest consumers to 1.28 mmol/L (95% confidence interval [CI]: 1.23, 1.33) among the highest (p-trend=0.007). Geometric mean triglyceride levels ranged from 0.79 mmol/L (95% CI: 0.72, 0.86) to 0.89 mmol/L (95% CI: 0.83, 0.96) (p-trend=0.03) with greater consumption of added sugars. Among those overweight/obese (≥85th percentile body-mass-index [BMI]), HOMA-IRs were positively associated with added sugars (p-linear trendadded sugars is positively associated with measures of cardiometabolic risk. Long-term studies are needed to determine if reduction in added sugars will improve these parameters and, thereby decrease future cardiovascular events. PMID:21220734

  2. 21 CFR 173.320 - Chemicals for controlling microorganisms in cane-sugar and beet-sugar mills.

    Science.gov (United States)

    2010-04-01

    ...-sugar and beet-sugar mills. 173.320 Section 173.320 Food and Drugs FOOD AND DRUG ADMINISTRATION... controlling microorganisms in cane-sugar and beet-sugar mills. Agents for controlling microorganisms in cane-sugar and beet-sugar mills may be safely used in accordance with the following conditions: (a) They are...

  3. Aluminum solubility and mobility in relation to organic carbon in surface soils affected by six tree species of the northeastern United States

    NARCIS (Netherlands)

    Dijkstra, F.A.; Fitzhugh, R.D.

    2003-01-01

    We compared Al solubility and mobility in surface soils among six tree species (sugar maple [Acer saccharum], white ash [Fraxinus americana], red maple [Acer rubrum, L.], American beech [Fagus grandifolia, Ehrh.], red oak [Quercus rubra, L.], and hemlock [Tsuga canadensis, Carr.]) in a mixed

  4. Reaction of simple sterile sugar beet hybrids to regulated environmental factors for sugar yield

    Directory of Open Access Journals (Sweden)

    М. О. Корнєєва

    2014-03-01

    Full Text Available Sugar yield, an important indicator, is resulting from interaction of two components: the yield and sugar content, both they are controlled by polygenes, and influenced significantly by environment. A.V. Kilchevskyi and L.V. Hotyliova pointed to the fact that in breeding, contrary to evolution, stabilizing forms are dominated by driving ones, which determine the growth response to regulated factors and decreased resistance to non-regulated environmental factors [1]. A relevant breeding trend is to create the genotypes adequate with certain technologies, so to say «low input variety high input variety» i.e. varieties with low and high energy contribution to technology. From this perspective, selection of stable in their manifestation lines against the analyzing backgrounds has become an important element of the breeding process [2]. Home scientists, such as A.L. Mazmulov and M.S. Hrytsyk have contributed greatly to the development and application of the agro-backgrounds [3, 4].

  5. Quantificação de açúcares solúveis totais, açúcares redutores e amido nos grãos verdes de cultivares de milho na colheita Quantification of total soluble sugars, reducing sugars and starch in immature kernels of corn cultivars

    Directory of Open Access Journals (Sweden)

    Fernanda Fátima Caniato

    2007-12-01

    Full Text Available O objetivo deste trabalho foi quantificar os teores de açúcares solúveis totais (AST, açúcares redutores (AR e amido em nove cultivares de milho colhidos no estádio verde, uma vez que estes influenciam diretamente o sabor, o aroma e a aceitação do consumidor. O experimento foi conduzido em Ponte Nova, MG, no ano agrícola 2002/2003. Para a determinação das características, foi retirada uma amostra de três espigas de cada unidade experimental. O ponto de colheita foi identificado por método empírico, quando o estilo-estigma se desprendia da espiga com facilidade. As cultivares apresentaram umidades diferentes no momento da colheita, variando de 56 a 64%. Não foi detectada diferença entre as cultivares quanto aos teores de AR, porém, P3232 e AG4051 apresentaram boa relação entre AR e AST, destacando-se para esta finalidade.Total soluble sugars (TSS, reducing sugars (RS, moisture and starch directly influence flavor and consumption. In this work, TSS, RS and starch were determined at harvest in young kernels of nine corn cultivars. The experiment was carried out in Ponte Nova, MG, Brazil. To determine chemical characters of young kernels a sample was obtained of three cobs from each experimental plot. Corn ears were harvested using an empirical method, when the silk was loose from the ear. Moisture varied among samples of different cultivars at harvest. Moisture content ranged from 56 to 64%. There were no differences among cultivars regarding RS. However, cultivars P3232 and AG4051 showed good RS to TSS ratio, being adequate for fresh consumption.

  6. Waste fatty acid addition to black liquor to decrease tall oil soap solubility and increase skimming efficiency in kraft mills pulping mountain pine beetle-infested wood

    Energy Technology Data Exchange (ETDEWEB)

    Uloth, V.; Guy, E. [FPInnovations, Prince George, BC (Canada). PAPRICAN Div.; Shewchuk, D. [Cariboo Pulp and Paper, Quesnel, BC (Canada); Van Heek, R. [Aker Kvaerner, Vancouver, BC (Canada)

    2009-07-01

    This paper presented the results of tests conducted to determine if the addition of waste fatty acids from vegetable oil processing might decrease tall oil soap solubility in pine-beetle impacted wood from British Columbia (BC). The soap recovery and tall oil production at BC mills has fallen by 30 to 40 percent in recent years due to the pulping of high proportions of grey-stage beetle-impacted wood. Full-scale mill tests were conducted over a 4-day period. The study showed that the addition of tall oil fatty acids or waste fatty acids from vegetable oil processing could decrease tall oil soap solubility and increase the soup skimming efficiency in mills pulping a large percentage of grey stage beetle-infested wood. The addition of fatty acids increased tall oil soap skimming efficiency from 50.2 percent in the baseline tests to 71.8 percent based on the total soap available, and from 76.7 percent in the baseline tests to 87.5 percent based on insoluble soap only. The economic analyses indicated that waste fatty acid addition could be economical when natural gas and oil prices are high. 4 tabs., 9 figs.

  7. Simulating multi-component liquid phase adsorption systems: ethanol and residual sugar

    Energy Technology Data Exchange (ETDEWEB)

    Jones, R.; Tezel, F.H.; Thibault, J. [Department of Chemical and Biological Engineering, University of Ottawa (Canada)], email: Jules.Thibault@uottawa.ca

    2011-07-01

    A series of multi-component adsorption studies was performed to determine the relative advantages of producing ethanol which is to be blended with gasoline. These studies developed a model to describe the competition for adsorption sites between ethanol and sugar molecules on the surface of the adsorbent. Three competitive adsorption models established by batch systems were examined to evaluate the suitability of the experiment data across different ethanol and sugar concentrations and determine their isotherm parameters. Multi-component packed bed adsorption experiments were then performed. The results show that ethanol capacity was decreased only slightly from that obtained in single component adsorption studies. There is significant evidence to indicate that sugar displacement from adsorption sites occurs because adsorption of ethanol is preferred. So the capacity of sugars will be greatly reduced if there are appreciable ethanol concentrations.

  8. Scoring function to predict solubility mutagenesis

    Directory of Open Access Journals (Sweden)

    Deutsch Christopher

    2010-10-01

    Full Text Available Abstract Background Mutagenesis is commonly used to engineer proteins with desirable properties not present in the wild type (WT protein, such as increased or decreased stability, reactivity, or solubility. Experimentalists often have to choose a small subset of mutations from a large number of candidates to obtain the desired change, and computational techniques are invaluable to make the choices. While several such methods have been proposed to predict stability and reactivity mutagenesis, solubility has not received much attention. Results We use concepts from computational geometry to define a three body scoring function that predicts the change in protein solubility due to mutations. The scoring function captures both sequence and structure information. By exploring the literature, we have assembled a substantial database of 137 single- and multiple-point solubility mutations. Our database is the largest such collection with structural information known so far. We optimize the scoring function using linear programming (LP methods to derive its weights based on training. Starting with default values of 1, we find weights in the range [0,2] so that predictions of increase or decrease in solubility are optimized. We compare the LP method to the standard machine learning techniques of support vector machines (SVM and the Lasso. Using statistics for leave-one-out (LOO, 10-fold, and 3-fold cross validations (CV for training and prediction, we demonstrate that the LP method performs the best overall. For the LOOCV, the LP method has an overall accuracy of 81%. Availability Executables of programs, tables of weights, and datasets of mutants are available from the following web page: http://www.wsu.edu/~kbala/OptSolMut.html.

  9. Payback time for soil carbon and sugar-cane ethanol

    Science.gov (United States)

    Mello, Francisco F. C.; Cerri, Carlos E. P.; Davies, Christian A.; Holbrook, N. Michele; Paustian, Keith; Maia, Stoécio M. F.; Galdos, Marcelo V.; Bernoux, Martial; Cerri, Carlos C.

    2014-07-01

    The effects of land-use change (LUC) on soil carbon (C) balance has to be taken into account in calculating the CO2 savings attributed to bioenergy crops. There have been few direct field measurements that quantify the effects of LUC on soil C for the most common land-use transitions into sugar cane in Brazil, the world's largest producer . We quantified the C balance for LUC as a net loss (carbon debt) or net gain (carbon credit) in soil C for sugar-cane expansion in Brazil. We sampled 135 field sites to 1 m depth, representing three major LUC scenarios. Our results demonstrate that soil C stocks decrease following LUC from native vegetation and pastures, and increase where cropland is converted to sugar cane. The payback time for the soil C debt was eight years for native vegetation and two to three years for pastures. With an increasing need for biofuels and the potential for Brazil to help meet global demand, our results will be invaluable for guiding expansion policies of sugar-cane production towards greater sustainability.

  10. A physiological and genetic approach to the improvement of tomato (Lycopersicon esculentum Mill.) fruit soluble solids

    International Nuclear Information System (INIS)

    Damon, S.E.

    1989-01-01

    Physiological processes and the genetic basis determining soluble solids content (SSC) of processing tomato fruit were addressed. Analysis of [ 3 H]-(fructosyl)-sucrose translocation in tomato indicates that phloem unloading in the fruit occurs, at least in part, to the apoplast. Apoplastic sucrose, glucose and fructose concentrations were estimated as 1 to 7, 12 to 49 and 8 to 63 millimolar, respectively in tomato fruit pericarp. Short-term uptake of [ 14 C]sucrose, -glucose and -fructose in tomato pericarp discs showes first order kinetics over the physiologically relevant concentration range. The uptake of [ 14 C]-(glycosyl)-1'fluorosucrose was identical to the rate of [ 14 C] sucrose uptake suggesting sucrose may be taken up directly without prior extracellular hydrolysis. Short-term uptake of all three sugars was insensitive to 10 micromolar carbonyl cyanide m-chlorophenylhydrazone and to 10 micromolar p-chloromercuribenzene sulfonic acid. However, long-term accumulation of glucose was sensitive to carbonyl cyanide m-chlorophenylhydrazone. Sugar uptake across the plasmamembrane does not appear to be energy dependent, suggesting that sugar accumulation in the tomato is driven by subsequent intracellular metabolism and/or active uptake at the tonoplast. Fourteen genomic DNA probes and ten restriction endonucleases were used to identify restriction fragment length polymorphisms (RFLPs) useful in the linkage analysis of quantitative trait loci controlling the expression of SSC in a segregating F 2 population from a cross between L. esculentum (UC204B) and L. cheesmanii f. minor, a wild species with high fruit soluble solids. RFLPs were detected between the DNAs of the two tomato species with all 14 probes

  11. One-step enzymatic synthesis of nucleosides from low water-soluble purine bases in non-conventional media.

    Science.gov (United States)

    Fernández-Lucas, Jesús; Fresco-Taboada, Alba; de la Mata, Isabel; Arroyo, Miguel

    2012-07-01

    The effect of several water-miscible cosolvents on activity and stability of soluble and immobilized 2'-deoxyribosyltransferase from Lactobacillus reuteri on Sepabeads® has been studied in order to establish optimal conditions for enzymatic synthesis of nucleosides using purine bases with low solubility in aqueous buffer. As a rule of thumb, there was a general reduction of soluble enzyme activity when cosolvent content was gradually increased in reaction medium. In contrast, immobilized enzyme activity was enhanced 1.2-1.4-fold at 20% of methanol, ethanol, 2-propanol, diethylene glycol, and acetone; and at 10% and 30% acetonitrile. Likewise, highest increased activity (1.8-fold) was also obtained in presence of 20% acetonitrile. Immobilized enzyme was successfully used in the synthesis of 2'-deoxyxanthosine and 2'-deoxyguanosine using 2'-deoxyuridine as sugar donor and the corresponding poor water-soluble base in the presence of 30% of methanol, ethanol, 2-propanol, ethylene glycol, acetonitrile, and DMSO, giving high nucleoside yields at 4h. Copyright © 2011 Elsevier Ltd. All rights reserved.

  12. Effect of storage on the physicochemical characteristics of the ...

    African Journals Online (AJOL)

    The experiment involved determination of the physicochemical compositions of moisture, total soluble solid (TSS), acidity, total sugar, reducing sugar, non ... Results indicate that increase in storage time increases the chemical compositions in the stored mangoes, except for acidity and fat that were decreased with the ...

  13. Method for determining the composition of the sugar moiety of a sugar containing compound

    DEFF Research Database (Denmark)

    2016-01-01

    The present invention relates to methods of labeling sugar moieties of sugar containing compounds including glycopeptides. The compounds presented in the present invention facilitate reliable detection of sugar moieties of sugar containing compounds by a combination of spectroscopy methods...

  14. STATISTICAL ANALYSIS OF RAW SUGAR MATERIAL FOR SUGAR PRODUCER COMPLEX

    OpenAIRE

    A. A. Gromkovskii; O. I. Sherstyuk

    2015-01-01

    Summary. In the article examines the statistical data on the development of average weight and average sugar content of sugar beet roots. The successful solution of the problem of forecasting these raw indices is essential for solving problems of sugar producing complex control. In the paper by calculating the autocorrelation function demonstrated that the predominant trend component of the growth raw characteristics. For construct the prediction model is proposed to use an autoregressive fir...

  15. SUGAR-SWEETENED BEVERAGE, SUGAR INTAKE OF INDIVIDUALS AND THEIR BLOOD PRESSURE: INTERMAP STUDY

    Science.gov (United States)

    Brown, Ian J.; Stamler, Jeremiah; Van Horn, Linda; Robertson, Claire E.; Chan, Queenie; Dyer, Alan R.; Huang, Chiang-Ching; Rodriguez, Beatriz L.; Zhao, Liancheng; Daviglus, Martha L.; Ueshima, Hirotsugu; Elliott, Paul

    2011-01-01

    The obesity epidemic has focused attention on relationships of sugars and sugar-sweetened beverages (SSB) to cardiovascular risk factors. Here we report cross-sectional associations of SSB, diet beverages, sugars with blood pressure (BP) for UK and USA participants of the International Study of Macro/Micro-nutrients and Blood Pressure (INTERMAP). Data collected includes four 24-h dietary recalls, two 24-h urine collections, eight BP readings, questionnaire data for 2,696 people ages 40-59 from 10 USA/UK population samples. Associations of SSB, diet beverages, and sugars (fructose, glucose, sucrose) with BP were assessed by multiple linear regression. Sugar-sweetened beverage intake related directly to BP, P-values 0.005 to Sugar-sweetened beverage intake higher by 1 serving/day (355 ml/24-h) was associated with systolic/diastolic BP differences of +1.6/+0.8 mm Hg (both P sugar-sodium interactions: for individuals with above-median 24-h urinary sodium excretion, fructose intake higher by 2 SD (5.6 %kcal) was associated with systolic/diastolic BP differences of +3.4/+2.2 mm Hg (both P sugar-BP differences for persons with higher sodium excretion, lend support to recommendations that intake of SSB, sugars, and salt be substantially reduced. PMID:21357284

  16. Biomass by-product from crystal sugar production: A comparative study between Ngadirejo and Mauritius sugar mill

    Science.gov (United States)

    Gunawan; Bantacut, T.; Romli, M.; Noor, E.

    2018-03-01

    Sugarcane has been used as raw material in crystal sugar industry. Sugar cane that contains high sugar will be utilized into crystals sugar. In addition, the productivity of sugarcane is large enough in 2016 approximately 360 713 tons/year. Crystal sugar itself is a daily necessity for its use in the food and beverage industry. Problem that occurs in Indonesia is the energy consumption. The sugar mills supposed to be an independent energy source which means it can produce its own energy by utilization the material that is available in the sugar mills such as by-product (bagasse, molasses, filter cake, etc.), the by product in every production stage are quite a lot in sugar industry especially in Indonesia. In this paper, a comparison between two sugar mills was examined between Ngedirejo sugar mill and Mauritius sugar mill which has the same geological state as Indonesia. The results of comparison between the two sugar factories demonstrated the difference in terms of productivity of the sugar that has been produced and the effectiveness of the production process in a sugar mill seen from the amount of waste and the by product.

  17. The effects of disordered structure on the solubility and dissolution rates of some hydrophilic, sparingly soluble drugs.

    Science.gov (United States)

    Mosharraf, M; Sebhatu, T; Nyström, C

    1999-01-15

    The effects of experimental design on the apparent solubility of two sparingly soluble hydrophilic compounds (barium sulphate and calcium carbonate) were studied in this paper. The apparent solubility appeared to be primarily dependent on the amount of solute added to the solvent in each experiment, increasing with increased amounts. This effect seems to be due to the existence of a peripheral disordered layer. However physico-chemical methods used in the present study were not able to unambiguously verify the existence of any disorder in the solid state structure of the drugs. At higher proportions of solute to solvent, the solubility reached a plateau corresponding to the solubility of the disordered or amorphous molecular form of the material. Milling the powders caused the plateau to be reached at lower proportions of solute to solvent, since this further disordered the surface of the drug particles. It was also found that the apparent solubility of the drugs tested decreased after storage at high relative humidities. A model for describing the effects of a disordered surface layer of varying thickness and continuity on the solubility of a substance is presented. This model may be used as a method for detection of minute amount of disorder, where no other technique is capable of detecting the disordered structure. It is suggested that recrystallisation of the material occurs via slow solid-state transition at the surface of the drug particle; this would slowly reduce the apparent solubility of the substance at the plateau level to the thermodynamically stable value. A biphasic dissolution rate profile was obtained. The solubility of the disordered surface of the particles appeared to be the rate-determining factor during the initial dissolution phase, while the solubility of the crystalline core was the rate-determining factor during the final slower phase.

  18. Study on Processing Technology and Quality of Moringa oleifera leaves with y - Aminobutyric Acid

    Directory of Open Access Journals (Sweden)

    Wei Zhou

    2017-01-01

    Full Text Available In order to obtain the high level of γ-aminobutyric acid Moringa oleifera leaves, Use 7% sodium glutamate solution to soak the fresh Moringa oleifera leaves, study effect of different treatment times and three different drying methods( hot air drying, vacuum freeze drying, shadow drying on the formation of y-aminobutyric acid and quality (total flavonoids, soluble sugar, amino acids, polyphenols, colorof dried Moringa oleifera leaves. The results indicated that shadow-dried Moringa oleifera leaves had the hightest retention of γ-aminobutyric acid, but its browning degree were not preferable, soluble sugar was damaged gravely, and its vulnerable to weather conditions. Vacuum freeze dried Moringa oleifera leaves had the hightest retention of flavonoids, polyphenols and amino acids. The y-aminobutyric acid content of Vacuum freeze dried and hot air dried Moringa oleifera leaves had no much difference. Hot air dried Moringa oleifera leaves browning degree were preferable, it’s had an moderate content of soluble sugar and amino acids, the short drying time is characteristics of this drying method.with the treatment time increased, the content of γ-aminobutyric acid and amino acids content first increased and then decreased. Flavonoids and polyphenols content first decreased and then increased. Soluble sugar content decreased. In summary, after soaking with 7% sodium glutamate solution for 10h, then dried by hot air drying(drying temperature of 60°C, was the most suitable way for industrial production of the high level of γ-aminobutyric acid Moringa oleifera leaves.

  19. Water-soluble dietary fibers and cardiovascular disease.

    Science.gov (United States)

    Theuwissen, Elke; Mensink, Ronald P

    2008-05-23

    One well-established way to reduce the risk of developing cardiovascular disease (CVD) is to lower serum LDL cholesterol levels by reducing saturated fat intake. However, the importance of other dietary approaches, such as increasing the intake of water-soluble dietary fibers is increasingly recognized. Well-controlled intervention studies have now shown that four major water-soluble fiber types-beta-glucan, psyllium, pectin and guar gum-effectively lower serum LDL cholesterol concentrations, without affecting HDL cholesterol or triacylglycerol concentrations. It is estimated that for each additional gram of water-soluble fiber in the diet serum total and LDL cholesterol concentrations decrease by -0.028 mmol/L and -0.029 mmol/L, respectively. Despite large differences in molecular structure, no major differences existed between the different types of water-soluble fiber, suggesting a common underlying mechanism. In this respect, it is most likely that water-soluble fibers lower the (re)absorption of in particular bile acids. As a result hepatic conversion of cholesterol into bile acids increases, which will ultimately lead to increased LDL uptake by the liver. Additionally, epidemiological studies suggest that a diet high in water-soluble fiber is inversely associated with the risk of CVD. These findings underlie current dietary recommendations to increase water-soluble fiber intake.

  20. The water footprint of sweeteners and bio-ethanol from sugar cane, sugar beet and maize

    NARCIS (Netherlands)

    Gerbens-Leenes, Winnie; Hoekstra, Arjen Ysbert

    2009-01-01

    Sugar cane and sugar beet are used for sugar for human consumption. In the US, maize is used, amongst others, for the sweetener High Fructose Maize Syrup (HFMS). Sugar cane, sugar beet and maize are also important for bio-ethanol production. The growth of crops requires water, a scarce resource. The

  1. Sugar palm (Argena pinnata). Potential of sugar palm for bio-ethanol production

    OpenAIRE

    Elbersen, H.W.; Oyen, L.P.A.

    2010-01-01

    The energetic and economic feasibility of bioethanol production from sugar palm is virtually unknown. A positive factor are the potentially very high yields while the long non-productive juvenile phase and the high labor needs can be seen as problematic. Expansion to large scale sugar palm cultivation comes with risks. Small-scale cultivation of sugar palm perfectly fits into local farming systems. In order to make a proper assessment of the value palm sugar as bio-ethanol crop more informati...

  2. Alfalfa root role in osmotic adjustment under salt stress (abstract)

    International Nuclear Information System (INIS)

    Ibriz, M.; Ghorri, M.; Alami, T.; El Guilli, M.; El- Moidaoui, M.; Benbella, M.

    2005-01-01

    The aim of this work was to evaluate the effect of the sodium chloride on the morpho physiological characteristics of Alfalfa (Medicago sativa L.). The characteristics taken into consideration dry matter production of shoot and root (DMS, DMR), root volume (RV), proline content (PS, PR), included total soluble sugar (SSS; SSR) and chlorophyll a, band (a+b). Salt tolerance of the six genotypes was characterised by capacity to growth in salt environment, buildup of osmoregulating compounds (proline and solubles sugar) and a less inhibition of photosynthesis process (decrease of chlorophyll pigment content). Important genotypes differences were observed for each parameter, which make possible a better understanding of the Alfalfa adaptation mechanisms. The results show that the salt stress has a significant influence on the growth of this plants by decreasing the production of dry matter and :)f the root volume. The most important decreases were clear at the 12 g/l concentration mainly upon the Australian variety (Siriver).Thus the most tolerant to salt stress was the Demnate genotype (Dem04) which presented the lowest decrease percentage. The salt effect upon the plant physiological characteristics causes a decrease of the relative water content and chlorophyll a, b and (a+b) content. It also causes an increase of the relative loss of water, the total soluble sugars (SSS; SSR) and the proline contents (PS, PR). Thus, we found a high correlation between the proline and sugar contents of shoot and root and also between these substances and shoot and root dry matter production. (author)

  3. Anaerobic Treatment of Cane Sugar Effluent from Muhoroni Sugar ...

    African Journals Online (AJOL)

    It was therefore concluded that anaerobic treatment, particularly with pH control and seeding shows potential in first stage management of sugar mill wastewater. Keywords: cane sugar mill effluent, anaerobic treatment, batch reactor, waste stabilization ponds. Journal of Civil Engineering Research and Practice Vol.

  4. Free Sugar Profile in Cycads

    Directory of Open Access Journals (Sweden)

    Thomas Edward Marler

    2014-10-01

    Full Text Available The sugars fructose, glucose, maltose, and sucrose were quantified in seven tissues of Zamia muricata Willd. to determine their distribution throughout various organs of a model cycad species, and in lateral structural roots of 18 cycad species to determine the variation in sugar concentration and composition among species representing every cycad genus. Taproot and lateral structural roots contained more sugars than leaf, stem, female strobilus, or coralloid roots. For example, taproot sugar concentration was 6.4-fold greater than stem sugar concentration. The dominant root sugars were glucose and fructose, and the only detected stem sugar was sucrose. Sucrose also dominated the sugar profile for leaflet and coralloid root tissue, and fructose was the dominant sugar in female strobilus tissue. Maltose was a minor constituent of taproot, leaflet, and female strobilus tissue, but absent in other tissues. The concentration of total free sugars and each of the four sugars did not differ among genera or families. Stoichiometric relationships among the sugars, such as the quotient hexoses/disaccharides, differed among organs and families. Although anecdotal reports on cycad starch have been abundant due to its historical use as human food and the voluminous medical research invested into cycad neurotoxins, this is the first report on the sugar component of the non-structural carbohydrate profile of cycads. Fructose, glucose, and sucrose are abundant in cycad tissues, with their relative abundance highly contrasting among organs. Their importance as forms of carbon storage, messengers of information, or regulators of cycad metabolism have not been determined to date.

  5. Rare sugars, d-allulose, d-tagatose and d-sorbose, differently modulate lipid metabolism in rats.

    Science.gov (United States)

    Nagata, Yasuo; Mizuta, Narumi; Kanasaki, Akane; Tanaka, Kazunari

    2018-03-01

    Rare sugars including d-allulose, d-tagatose, and d-sorbose are present in limited quantities in nature; some of these rare sugars are now commercially produced using microbial enzymes. Apart from the anti-obesity and anti-hyperglycaemic activities of d-allulose, effects of these sugars on lipid metabolism have not been investigated. Therefore, we aimed to determine if and how d-tagatose and d-sorbose modulate lipid metabolism in rats. After feeding these rare sugars to rats, parameters on lipid metabolism were determined. No diet-related effects were observed on body weight and food intake. Hepatic lipogenic enzyme activity was lowered by d-allulose and d-sorbose but increased by d-tagatose. Faecal fatty acid excretion was non-significantly decreased by d-allulose, but significantly increased by d-sorbose without affecting faecal steroid excretion. A trend toward reduced adipose tissue weight was observed in groups fed rare sugars. Serum adiponectin levels were decreased by d-sorbose relative to the control. Gene expression of cholesterol metabolism-related liver proteins tended to be down-regulated by d-allulose and d-sorbose but not by d-tagatose. In the small intestine, SR-B1 mRNA expression was suppressed by d-sorbose. Lipid metabolism in rats varies with rare sugars. Application of rare sugars to functional foods for healthy body weight maintenance requires further studies. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  6. Home blood sugar testing

    Science.gov (United States)

    Diabetes - home glucose testing; Diabetes - home blood sugar testing ... Usual times to test your blood sugar are before meals and at bedtime. Your provider may ask you to check your blood sugar 2 hours after a meal or ...

  7. Sugar from Palms

    DEFF Research Database (Denmark)

    Barfod, Anders

    Throughout the tropics and subtropics a large number of products are derived from the sugar-rich sap tapped from palms. I will give an overview of the most important species being exploited, harvesting practices and yields. I will further provide insights in the biomechanmics of sugar...... transportation in palms, which remain an enigma. Finally, the prospects for developing palm sugar into a commodity of worlswide significance will be discussed....

  8. Sugar maple growth in relation to nutrition and stress in the northeastern United States.

    Science.gov (United States)

    Long, Robert P; Horsley, Stephen B; Hallett, Richard A; Bailey, Scott W

    2009-09-01

    Sugar maple, Acer saccharum, decline disease is incited by multiple disturbance factors when imbalanced calcium (Ca), magnesium (Mg), and manganese (Mn) act as predisposing stressors. Our objective in this study was to determine whether factors affecting sugar maple health also affect growth as estimated by basal area increment (BAI). We used 76 northern hardwood stands in northern Pennsylvania, New York, Vermont, and New Hampshire, USA, and found that sugar maple growth was positively related to foliar concentrations of Ca and Mg and stand level estimates of sugar maple crown health during a high stress period from 1987 to 1996. Foliar nutrient threshold values for Ca, Mg, and Mn were used to analyze long-term BAI trends from 1937 to 1996. Significant (P maples sampled in the 1990s had decreased growth in the 1970s, 10-20 years in advance of the 1980s and 1990s decline episode in Pennsylvania. Even apparently healthy stands that had no defoliation, but had below-threshold amounts of Ca or Mg and above-threshold Mn (from foliage samples taken in the mid 1990s), had decreasing growth by the 1970s. Co-occurring black cherry, Prunus serotina, in a subset of the Pennsylvania and New York stands, showed opposite growth responses with greater growth in stands with below-threshold Ca and Mg compared with above-threshold stands. Sugar maple growing on sites with the highest concentrations of foliar Ca and Mg show a general increase in growth from 1937 to 1996 while other stands with lower Ca and Mg concentrations show a stable or decreasing growth trend. We conclude that acid deposition induced changes in soil nutrient status that crossed a threshold necessary to sustain sugar maple growth during the 1970s on some sites. While nutrition of these elements has not been considered in forest management decisions, our research shows species specific responses to Ca and Mg that may reduce health and growth of sugar maple or change species composition, if not addressed.

  9. Cross-linking of wheat gluten using a water-soluble carbodiimide

    NARCIS (Netherlands)

    Tropini, V.; Lens, J.P.; Mulder, W.J.; Silvestre, F.

    2000-01-01

    Wheat gluten was cross-linked using water-soluble 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC). To enhance cross-linking, N-hydroxysuccinimide (NHS) was added to the reaction mixture. The cross-linking efficiency was evaluated by the decrease in the amount of amino groups, the solubility

  10. Sugar Restriction Leads to Increased Ad Libitum Sugar Intake by Overweight Adolescents in an Experimental Test Meal Setting.

    Science.gov (United States)

    O'Reilly, Gillian A; Black, David S; Huh, Jimi; Davis, Jaimie N; Unger, Jennifer; Spruijt-Metz, Donna

    2017-07-01

    The impact of sugar restriction on subsequent sugar intake by overweight adolescents is unknown. Our aim was to examine the effect of sugar restriction on subsequent ad libitum sugar intake by overweight adolescents and whether habitual sugar intake and impulsivity influence the effect of sugar restriction on subsequent sugar intake. This was an in-laboratory crossover feeding trial with sugar-exposure and sugar-restriction conditions. Eighty-seven overweight Latino and African-American adolescents underwent both meal conditions in two separate 8-hour in-laboratory visits. Participants had access to ad libitum snack trays for 3 hours after the condition-specific meals. Ad libitum sugar intake during the snack period was measured at each visit. Habitual sugar intake and impulsivity were assessed at baseline. Repeated measures analysis of covariance was used to examine the within-person effect of meal condition on ad libitum sugar intake. Mixed models were used to examine the moderating effects of habitual sugar intake and impulsivity on the meal condition-ad libitum sugar intake relationship. Participants consumed more ad libitum sugar during the snack period in the sugar-restriction condition than in the sugar-exposure condition (sugar restriction=78.63±38.84 g, sugar exposure=70.86±37.73 g; F=9.64, P=0.002). There was no relationship between habitual sugar intake and how much ad libitum sugar participants consumed during either condition. Higher impulsivity was associated with greater ad libitum sugar intake during both conditions (sugar restriction: b=.029, standard error=.01, Poverweight adolescents restricted from sugar intake consume greater amounts of sugar when they are later given access to high-sugar foods. Overweight adolescents with higher impulsivity appear to consume greater amounts of sugar regardless of previous levels of sugar consumption. Compensatory sugar intake and trait impulsivity may have implications for dietary interventions in this

  11. Complete oxidative conversion of lignocellulose derived non-glucose sugars to sugar acids by Gluconobacter oxydans.

    Science.gov (United States)

    Yao, Ruimiao; Hou, Weiliang; Bao, Jie

    2017-11-01

    Non-glucose sugars derived from lignocellulose cover approximately 40% of the total carbohydrates of lignocellulose biomass. The conversion of the non-glucose sugars to the target products is an important task of lignocellulose biorefining research. Here we report a fast and complete conversion of the total non-glucose sugars from corn stover into the corresponding sugar acids by whole cell catalysis and aerobic fermentation of Gluconobacter oxydans. The conversions include xylose to xylonate, arabinose to arabonate, mannose to mannonate, and galactose to galactonate, as well as with glucose into gluconate. These cellulosic non-glucose sugar acids showed the excellent cement retard setting property. The mixed cellulosic sugar acids could be used as cement retard additives without separation. The conversion of the non-glucose sugars not only makes full use of lignocellulose derived sugars, but also effectively reduces the wastewater treatment burden by removal of residual sugars. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Changes in polyphenol and sugar concentrations in wild type and genetically modified Nicotiana langsdorffii Weinmann in response to water and heat stress.

    Science.gov (United States)

    Ancillotti, Claudia; Bogani, Patrizia; Biricolti, Stefano; Calistri, Elisa; Checchini, Leonardo; Ciofi, Lorenzo; Gonnelli, Cristina; Del Bubba, Massimo

    2015-12-01

    In this study wild type Nicotiana langsdorffii plants were genetically transformed by the insertion of the rat gene (gr) encoding the glucocorticoid receptor or the rolC gene and exposed to water and heat stress. Water stress was induced for 15 days by adding 20% PEG 6000 in the growth medium, whereas the heat treatment was performed at 50 °C for 2 h, after that a re-growing capability study was carried out. The plant response to stress was investigated by determining electrolyte leakage, dry weight biomass production and water content. These data were evaluated in relation to antiradical activity and concentrations of total polyphenols, selected phenolic compounds and some soluble sugars, as biochemical indicators of metabolic changes due to gene insertion and/or stress treatments. As regards the water stress, the measured physiological parameters evidenced an increasing stress level in the order rolC < gr < WT plants (e.g. about 100% and 50% electrolyte leakage increase in WT and gr samples, respectively) and complied with the biochemical pattern, which consisted in a general decrease of antiradical activity and phenolics, together with an increase in sugars. As regard heat stress, electrolyte leakage data were only in partial agreement with the re-growing capability study. In fact, according to this latter evaluation, gr was the genotype less affected by the heat shock. In this regard, sugars and especially phenolic compounds are informative of the long-term effects due to heat shock treatment. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  13. Atmospheric sugar alcohols: evaporation rates and saturation vapor pressures

    DEFF Research Database (Denmark)

    Bilde, Merete; Zardini, Alessandro Alessio; Hong, Juan

    alcohols. These polyols are common in the water soluble fraction of atmospheric aerosols. In our experimental system sub-micron particles are generated by nebulization from aqueous solution, and a mono disperse fraction of the aerosol is selected using a differential mobility analyzer. The particles......The atmospheric partitioning between gas and condensed phase of organic molecules is poorly understood, and discrepancies exist between predicted and observed concentrations of secondary organic aerosols. A key problem is the lack of information about thermodynamic properties of semi- and low...... volatile organic molecules. Saturation vapor pressure and the associated temperature dependence (dH) are key parameters for improving predictive atmospheric models. In this work we combine experiments and thermodynamic modeling to investigate these parameters for a series of polyols, so-called sugar...

  14. Respiration-dependent utilization of sugars in yeasts: a determinant role for sugar transporters.

    Science.gov (United States)

    Goffrini, Paola; Ferrero, Iliana; Donnini, Claudia

    2002-01-01

    In many yeast species, including Kluyveromyces lactis, growth on certain sugars (such as galactose, raffinose, and maltose) occurs only under respiratory conditions. If respiration is blocked by inhibitors, mutation, or anaerobiosis, growth does not take place. This apparent dependence on respiration for the utilization of certain sugars has often been suspected to be associated with the mechanism of the sugar uptake step. We hypothesized that in many yeast species, the permease activities for these sugars are not sufficient to ensure the high substrate flow that is necessary for fermentative growth. By introducing additional sugar permease genes, we have obtained K. lactis strains that were capable of growing on galactose and raffinose in the absence of respiration. High dosages of both the permease and maltase genes were indeed necessary for K. lactis cells to grow on maltose in the absence of respiration. These results strongly suggest that the sugar uptake step is the major bottleneck in the fermentative assimilation of certain sugars in K. lactis and probably in many other yeasts.

  15. 76 FR 50285 - Fiscal Year 2012 Tariff-Rate Quota Allocations for Raw Cane Sugar, Refined and Specialty Sugar...

    Science.gov (United States)

    2011-08-12

    ... for Raw Cane Sugar, Refined and Specialty Sugar and Sugar-Containing Products AGENCY: Office of the... quantity of the tariff-rate quotas for imported raw cane sugar, refined and specialty sugar and sugar...), the United States maintains tariff-rate quotas (TRQs) for imports of raw cane sugar and refined sugar...

  16. Sugar Price Supports and Taxation: A Public Health Policy Paradox.

    Science.gov (United States)

    Dilk, Abby; Savaiano, Dennis A

    2017-05-01

    Domestic US sugar production has been protected by government policy for the past 82 years, resulting in elevated domestic prices and an estimated annual (2013) $1.4 billion dollar "tax" on consumers. These elevated prices and the simultaneous federal support for domestic corn production have ensured a strong market for high-fructose corn syrup. Americans have dramatically increased their consumption of caloric sweeteners during the same period. Consumption of "empty" calories (ie, foods with low-nutrient/high-caloric density)-sugar and high-fructose corn syrup being the primary sources-is considered by most public health experts to be a key contributing factor to the rise in obesity. There have been substantial efforts to tax sugar-sweetened beverages (SSBs) to both reduce consumption and provide a source of funds for nutrition education, thereby emulating the tobacco tax model. Volume-based SSB taxes levy the tax rate per ounce of liquid, where some are only imposed on beverages with added sugar content exceeding a set threshold. Nonetheless, volume-based taxes have significant limitations in encouraging consumers to reduce their caloric intake due to a lack of transparency at the point of purchase. Thus, it is hypothesized that point-of-purchase, nutrient-specific excise taxes on SSBs would be more effective at reducing sugar consumption. However, all SSB taxes are limited by the possibility that consumers may compensate their decreased intake from SSBs with other high-calorie junk foods. Furthermore, there are no existing studies to provide evidence on how SSB taxes will impact obesity rates in the long term. The paradox of sugar prices is that Americans have paid higher prices for sugar to protect domestic production for more than 80 years, and now, Americans are being asked to pay even more to promote public health. The effective use of sugar taxes should be considered based on their merits in reducing sugar consumption and making available a new source of

  17. Ray tissues as an indirect measure of relative sap-sugar concentration in sugar maple

    Science.gov (United States)

    Peter W. Garrett; Kenneth R. Dudzik; Kenneth R. Dudzik

    1989-01-01

    Attempts to correlate ray tissue as a percentage of total wood volume with sap-sugar concentrations of sugar maple progenies were unsuccessful. These results raise doubts about our ability to use a relatively constant value such as ray-tissue volume in a selection program designed to increase the sap-sugar concentration of sugar maple seedlings.

  18. Effect of Domestic Processing Methods on Dry Matter, Total Sugar, Phenolics and Mineral Composition in Different Developmental Stages of Parkia roxburghii G. (Don. Pods

    Directory of Open Access Journals (Sweden)

    Jekendra Singh SALAM

    2013-02-01

    Full Text Available Parkia roxburghii is considered delicious in the Northeastern part of India, especially in the state of Manipur. Though it is widely used, information about the biochemical composition and its changes, after processing, is hardly available. In the present experiment, effect of processing methods on dry matter, soluble sugar, phenolics and mineral composition in different developmental stages of P. roxburghii pods were studied. Total soluble sugar (TSS, total phenols (TP, and orthodihydric phenols (ODHP were determined as per the methods of Morris (1948, Bray and Thorpe (1954 and Mahadevan and Sridhar (1986. Sodium (Na, potassium (K, sulphur (S and phosphorus (P were estimated as per the procedures of Tandon (1993. Calcium (Ca, magnesium (Mg, manganese (Mn, zinc (Zn, iron (Fe, copper (Cu and cobalt (Co were analyzed in an atomic absorption spectrophotometer. Processing methods reduced dry matter, soluble sugar and phenolics in all the stages of the pod. TP and ODHP lost up to the extent of 72.78% in tender stage due to ordinary cooking, while higher loss of ODHP recorded in immature stage of the pod due to pressure cooking. Ca, Mg, K and Zn in different stages were found to be affected significantly by different methods of cooking, while no such changes were observed in case of S, P, Fe, Mn and Cu. The level of iron amongst the micro minerals is appreciably high recording up to 51.0 mg/100 g in the immature stage of the pod. As iron, zinc and manganese are antioxidant micronutrients, their higher presence in P. roxburghii might be of some nutritional importance.

  19. 77 FR 57180 - Fiscal Year 2013 Tariff-rate Quota Allocations for Raw Cane Sugar, Refined and Specialty Sugar...

    Science.gov (United States)

    2012-09-17

    ... Sugar, Refined and Specialty Sugar, and Sugar-Containing Products AGENCY: Office of the United States... quantity of the tariff-rate quotas for imported raw cane sugar, refined and specialty sugar, and sugar... imports of raw cane sugar and refined sugar. Pursuant to Additional U.S. Note 8 to Chapter 17 of the HTS...

  20. Apparatus for drying sugar cubes

    NARCIS (Netherlands)

    Derckx, H.A.J.; Torringa, H.M.

    1999-01-01

    Device for drying sugar cubes containing a heating apparatus for heating and dehumidifying the sugar cubes, a conditioning apparatus for cooling off and possibly further dehumidifying the sugar cubes and a conveying apparatus for conveying the sugar cubes through the heating apparatus and the

  1. Influence of calcium and phosphorus, lactose, and salt-to-moisture ratio on Cheddar cheese quality: changes in residual sugars and water-soluble organic acids during ripening.

    Science.gov (United States)

    Upreti, P; McKay, L L; Metzger, L E

    2006-02-01

    Cheddar cheese ripening involves the conversion of lactose to glucose and galactose or galactose-6-phosphate by starter and nonstarter lactic acid bacteria. Under ideal conditions (i.e., where bacteria grow under no stress of pH, water activity, and salt), these sugars are mainly converted to lactic acid. However, during ripening of cheese, survival and growth of bacteria occurs under the stressed condition of low pH, low water activity, and high salt content. This forces bacteria to use alternate biochemical pathways resulting in production of other organic acids. The objective of this study was to determine if the level and type of organic acids produced during ripening was influenced by calcium (Ca) and phosphorus (P), residual lactose, and salt-to-moisture ratio (S/M) of cheese. Eight cheeses with 2 levels of Ca and P (0.67 and 0.47% vs. 0.53 and 0.39%, respectively), lactose at pressing (2.4 vs. 0.78%), and S/M (6.4 vs. 4.8%) were manufactured. The cheeses were analyzed for organic acids (citric, orotic, pyruvic, lactic, formic, uric, acetic, propanoic, and butyric acids) and residual sugars (lactose, galactose) during 48 wk of ripening using an HPLC-based method. Different factors influenced changes in concentration of residual sugars and organic acids during ripening and are discussed in detail. Our results indicated that the largest decrease in lactose and the largest increase in lactic acid occurred between salting and d 1 of ripening. It was interesting to observe that although the lactose content in cheese was influenced by several factors (Ca and P, residual lactose, and S/M), the concentration of lactic acid was influenced only by S/M. More lactic acid was produced in low S/M treatments compared with high S/M treatments. Although surprising for Cheddar cheese, a substantial amount (0.2 to 0.4%) of galactose was observed throughout ripening in all treatments. Minor changes in the levels of citric, uric, butyric, and propanoic acids were observed during

  2. Process evaluation of enzymatic hydrolysis with filtrate recycle for the production of high concentration sugars.

    Science.gov (United States)

    Xue, Ying; Rusli, Jannov; Chang, Hou-Min; Phillips, Richard; Jameel, Hasan

    2012-02-01

    Process simulation and lab trials were carried out to demonstrate and confirm the efficiency of the concept that recycling hydrolysate at low total solid enzymatic hydrolysis is one of the options to increase the sugar concentration without mixing problems. Higher sugar concentration can reduce the capital cost for fermentation and distillation because of smaller retention volume. Meanwhile, operation cost will also decrease for less operating volume and less energy required for distillation. With the computer simulation, time and efforts can be saved to achieve the steady state of recycling process, which is the scenario for industrial production. This paper, to the best of our knowledge, is the first paper discussing steady-state saccharification with recycling of the filtrate form enzymatic hydrolysis to increase sugar concentration. Recycled enzymes in the filtrate (15-30% of the original enzyme loading) resulted in 5-10% higher carbohydrate conversion compared to the case in which recycled enzymes were denatured. The recycled hydrolysate yielded 10% higher carbohydrate conversion compared to pure sugar simulated hydrolysate at the same enzyme loading, which indicated hydrolysis by-products could boost enzymatic hydrolysis. The high sugar concentration (pure sugar simulated) showed inhibition effect, since about 15% decrease in carbohydrate conversion was observed compared with the case with no sugar added. The overall effect of hydrolysate recycling at WinGEMS simulated steady-state conditions with 5% total solids was increasing the sugar concentration from 35 to 141 g/l, while the carbohydrate conversion was 2% higher for recycling at steady state (87%) compared with no recycling strategy (85%). Ten percent and 15% total solid processes were also evaluated in this study.

  3. Short–Term Exposure to Electromagnetic Fields Generated by Mobile Phone Jammers Decreases the Fasting Blood Sugar in Adult Male Rats

    Directory of Open Access Journals (Sweden)

    Shekoohi Shooli F.

    2016-03-01

    Full Text Available Background: Substantial evidence indicates that exposure to electromagnetic fields (EMF above certain levels can affect human health through triggering some biological responses. According to WHO, short-term exposure to EMF at the levels present in the home/environment do not cause any apparent detrimental effects in healthy individuals. However, now, there is a debate on whether long-term exposure to low level EMF can evoke detrimental biological responses. Although based on the Communications Act of 1934, selling, advertising, using, or importing mobile jammers which block cell phone calls and text messages are illegal acts, in some countries these devices are being used for security purpose and for prevention of cheating during examinations. Methods: In this study 30 male Wistar rats were randomly divided into 3 groups of 10 each. The control group received no radiation. The sham exposure group was exposed to a switched-off jammer device. After fasting for 12 hours, the exposure group was exposed to EMFs at a distance of 50 cm from the jammer. Blood samples were collected from the tail vein after 24, 48 and72 hours and fasting blood sugar was measured by using a common blood glucose monitor (BIONIME GM110, Taiwan. The significance level was considered 5% and SPSS Ver. 21 was used for statistical analysis. The data were analyzed by ANOVA followed by Tukey’s test. Results: A statistically significant difference was observed between blood sugar level in the control and exposure groups after 24, 48 and 72 hours of continuous irradiation (p values were <0.001, <0.001 and 0.002, respectively. No significant difference was found between the level of fasting blood sugar in control and sham groups. Conclusion: Short-term exposure to electromagnetic field generated by mobile phone jammer can reduce blood sugar level in adult male rats. These findings, in contrast with our previous results, lead us to this conclusion that the use of these signal

  4. Antagonism of the morphine-induced locomotor activation of mice by fructose: comparison with other opiates and sugars, and sugar effects on brain morphine.

    Science.gov (United States)

    Brase, D A; Ward, C R; Bey, P S; Dewey, W L

    1991-01-01

    The mouse locomotor activation test of opiate action in a 2+2 dose parallel line assay was used in a repeated testing paradigm to determine the test, opiate and hexose specificities of a previously reported antagonism of morphine-induced antinocociception by hyperglycemia. In opiate specificity studies, fructose (5 g/kg, i.p.) significantly reduced the potency ratio for morphine and methadone, but not for levorphanol, meperidine or phenazocine when intragroup comparisons were made. In intergroup comparisons, fructose significantly reduced the potencies of levorphanol and phenazocine, but not methadone or meperidine. In hexose/polyol specificity studies, tagatose and fructose significantly reduced the potency ratio for morphine, whereas glucose, galactose, mannose and the polyols, sorbitol and xylitol, caused no significant decrease in potency. Fructose, tagatose, glucose and mannose (5 g/kg, i.p.) were tested for effects on brain morphine levels 30 min after morphine (60 min after sugar), and all four sugars significantly increased brain morphine relative to saline-pretreated controls. It is concluded that the antagonism of morphine by acute sugar administration shows specificity for certain sugars and occurs despite sugar-induced increases in the distribution of morphine to the brain. Furthermore, the effects of fructose show an opiate specificity similar to that of glucose on antinociception observed previously in our laboratory, except that methadone was also significantly inhibited in the present study, when a repeated-testing experimental design was used.

  5. GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence.

    Directory of Open Access Journals (Sweden)

    Miki Kawada-Matsuo

    Full Text Available Streptococcus mutans is a cariogenic pathogen that produces an extracellular polysaccharide (glucan from dietary sugars, which allows it to establish a reproductive niche and secrete acids that degrade tooth enamel. While two enzymes (GlmS and NagB are known to be key factors affecting the entrance of amino sugars into glycolysis and cell wall synthesis in several other bacteria, their roles in S. mutans remain unclear. Therefore, we investigated the roles of GlmS and NagB in S. mutans sugar metabolism and determined whether they have an effect on virulence. NagB expression increased in the presence of GlcNAc while GlmS expression decreased, suggesting that the regulation of these enzymes, which functionally oppose one another, is dependent on the concentration of environmental GlcNAc. A glmS-inactivated mutant could not grow in the absence of GlcNAc, while nagB-inactivated mutant growth was decreased in the presence of GlcNAc. Also, nagB inactivation was found to decrease the expression of virulence factors, including cell-surface protein antigen and glucosyltransferase, and to decrease biofilm formation and saliva-induced S. mutans aggregation, while glmS inactivation had the opposite effects on virulence factor expression and bacterial aggregation. Our results suggest that GlmS and NagB function in sugar metabolism in opposing directions, increasing and decreasing S. mutans virulence, respectively.

  6. Fenton reagent and titanium dioxide nanoparticles as antifungal agents to control leaf spot of sugar beet under field conditions

    Directory of Open Access Journals (Sweden)

    Hamza Amany

    2016-07-01

    Full Text Available In this study, foliar sprays of Fenton solutions (Fenton reaction, Fenton-like reaction and Fenton complex, titanium dioxide (TiO2 and the recommended fungicide (chlorothalonil were estimated in the control of sugar beet leaf spot caused by Cercospora beticola under field conditions in two growing seasons. In addition, the impacts of these treatments on some crop characters (leaf dry weight, root fresh weight, soluble solid content, sucrose content and purity of sugar were examined. Biochemical and histological changes in the livers and kidneys of treated rats compared to an untreated control were utilized to assess the toxicity of the examined curative agents. Overall, chlorothalonil and Fenton complex were the most effective treatments for disease suppression in both tested seasons followed by Fenton-like reagent, Fenton’s reagent and TiO2, respectively. Growth and yield characters of treated sugar beet significantly increased in comparison to an untreated control. There were mild or no (biochemical and histological changes in the livers and kidneys of treated rats compared to the control. Fenton solutions and TiO2 may offer a new alternative for leaf spot control in sugar beet.

  7. Sugar consumption, metabolic disease and obesity: The state of the controversy

    Science.gov (United States)

    Stanhope, Kimber L.

    2016-01-01

    The impact of sugar consumption on health continues to be a controversial topic. The objective of this review is to discuss the evidence and lack of evidence that allows the controversy to continue, and why resolution of the controversy is important. There are plausible mechanisms and research evidence that support the suggestion that consumption of excess sugar promotes the development of cardiovascular disease (CVD) and type 2 diabetes (T2DM) both directly and indirectly. The direct pathway involves the unregulated hepatic uptake and metabolism of fructose, which leads to liver lipid accumulation, dyslipidemia, decreased insulin sensitivity and increased uric acid levels. The epidemiological data suggest that these direct effects of fructose are pertinent to the consumption of the fructose-containing sugars, sucrose and HFCS, which are the predominant added sugars. Consumption of added sugar is associated with development and/or prevalence of fatty liver, dyslipidemia, insulin resistance, hyperuricemia, cardiovascular disease and type 2 diabetes, and many of these associations are independent of body weight gain or total energy intake. There are diet intervention studies in which human subjects exhibited increased circulating lipids and decreased insulin sensitivity when consuming high sugar compared with control diets. Most recently, our group has reported that supplementing the ad libitum diets of young adults with beverages containing 0, 10, 17.5 or 25% of daily energy requirement (Ereq) as high fructose corn syrup (HFCS) increased lipid/lipoprotein risk factors for cardiovascular disease (CVD) and uric acid in a dose response manner. However, un-confounded studies conducted in healthy humans under a controlled, energy-balanced diet protocol that allow determination of the effects of sugar with diets that do not allow for body weight gain are lacking. Furthermore, there are recent reports that conclude that there are no adverse effects of consuming beverages

  8. Determination and correlation of the solubility for diosgenin in alcohol solvents

    International Nuclear Information System (INIS)

    Chen Feixiong; Zhao Mingrui; Liu Chuochuo; Peng Feifei; Ren Baozeng

    2012-01-01

    Highlights: ► The solubilities of diosgenin in different alcohols solvents have been obtained. ► The solubility decreases with the increase of the polarity of the alcohols solvents. ► The results show that the three models agree well with the experimental data. - Abstract: Using a laser monitoring technique, the solubility of diosgenin in ethanol, 1-propanol, 1-butanol, isobutyl alcohol, tert-butanol, 1-pentanol, and iso-octyl alcohol was measured over the temperature range from (290.15 to 330.15) K at atmospheric pressure. Its corresponding (solid + liquid) equilibrium data will provide essential support for industrial design and further theoretical studies. From the experimental results, the solubility of diosgenin in ethanol, 1-propanol, 1-butanol, isobutyl alcohol, tert-butanol, 1-pentanol, and iso-octyl alcohol was found to increase with increasing temperature and decrease with the increase of the polarity of the alcohols solvents. The Apelblat equation, the ideal model and the λh equation were used to correlate the solubility values. The results showed that the three models mentioned above agreed well with the experimental data.

  9. The Sugar Tax in Holland

    NARCIS (Netherlands)

    Ajjaji, Fadoua

    2016-01-01

    This inquiry supports the theory of a sugar tax has a positive influence on the sugar consumption of Dutch individuals. Once a tax is implemented, the sugar consumption declines. Furthermore, this study supported the hypothesis claiming that children have a positive influence on their parental sugar

  10. Sugar - a harmless indulgence?

    DEFF Research Database (Denmark)

    Rasmussen, Lone Banke; Andersen, Niels Lyhne; Ovesen, L.

    1998-01-01

    The consumption of sugar is relatively high in Denmark - and other industrial countries - and many persons have a consumption which exceeds the recommended level of maximally 10% of energy intake. A high sugar consumption may reduce the nutrient density of the diet and increase the risk of vitamin...... and mineral deficiency, especially in low energy consumers. The sugar intake and the fat intake, expressed as percentage of energy, usually show an inverse association. This has lead to the statement that a diet with both a low sugar content and a low fat content is incompatible, but we will argue...... that this is not the fact. The significance of sugar for the development of obesity is not clarified. A high fat content in the diet seems to promote the development of obesity, while a high carbohydrate content tends to reduce obesity. It is not known if sugar in this connection is comparable to the other carbohydrates...

  11. Elevated temperature altered photosynthetic products in wheat seedlings and organic compounds and biological activity in rhizopshere soil under cadmium stress

    Science.gov (United States)

    Jia, Xia; Zhao, Yonghua; Wang, Wenke; He, Yunhua

    2015-09-01

    The objective of this study was to investigate the effects of slightly elevated atmospheric temperature in the spring on photosynthetic products in wheat seedlings and on organic compounds and biological activity in rhizosphere soil under cadmium (Cd) stress. Elevated temperature was associated with increased soluble sugars, reducing sugars, starch, and total sugars, and with decreased amino acids in wheat seedlings under Cd stress. Elevated temperature improved total soluble sugars, free amino acids, soluble phenolic acids, and organic acids in rhizosphere soil under Cd stress. The activity of amylase, phenol oxidase, invertase, β-glucosidase, and L-asparaginase in rhizosphere soil was significantly improved by elevated temperature under Cd stress; while cellulase, neutral phosphatase, and urease activity significantly decreased. Elevated temperature significantly improved bacteria, fungi, actinomycetes, and total microorganisms abundance and fluorescein diacetate activity under Cd stress. In conclusion, slightly elevated atmospheric temperature in the spring improved the carbohydrate levels in wheat seedlings and organic compounds and biological activity in rhizosphere soil under Cd stress in the short term. In addition, elevated atmospheric temperature in the spring stimulated available Cd by affecting pH, DOC, phenolic acids, and organic acids in rhizosphere soil, which resulted in the improvement of the Cd uptake by wheat seedlings.

  12. Corporate Governance of Sugar Mills in East Java: A Transaction Cost Economics Perspective

    Directory of Open Access Journals (Sweden)

    Ahmad Erani Yustika

    2007-01-01

    Full Text Available Despite Indonesia’s overall achievements during the past two decades%2C the economy is encountering a series of problems. One of the biggest challenges is the unsatisfactory performance of the state-owned enterprises (SOEs. Subsidisies and uncovered loans to the SOEs have drained the government’s fiscal resources%2C and the signing off of employees creates many social problems. Compared with the SOEs in other sectors%2C state-owned sugar mills face more serious crises which not only jeopardise the social fabric%2C but also endanger the production. Research results strengthen the statement that the basic problem in sugar mills is management inefficiency resulting in high transaction costs. This research compares the transaction costs between state-owned (Ngadiredjo and privately-owned (Kebon Agung sugar mills. The study shows that in Kebon Agung Sugar Mill transaction costs are higher than production costs%2C while in Ngadiredjo Sugar Mill the reverse is true. However%2C the high transaction costs in Kebon Agung Sugar Mill cannot be attributed directly to inefficient institutions%2C because Ngadiredjo Sugar Mill incurred high costs for plants%2C land preparation%2C and fertilizer%2C which decreased the proportion of transaction costs. If analyzed in detail%2C the following facts are revealed: (i market transaction costs in Kebon Agung Sugar Mill are higher than in Ngadiredjo Sugar Mill. This is because Kebon Agung Sugar Mill has established cooperation with sugarcane farmers in the form of extensions and transport subsidies; and (ii the political transaction costs proportion in Ngadiredjo Sugar Mill is higher than in Kebon Agung Sugar Mill because of the imposition of many ‘illegal’ fees. Abstract in Bahasa Indonesia : transaction cost economics%2C corporate governance%2C sugar mill%2C East Java.

  13. [Effects of exogenous nitric oxide on physiological characteristics of longan (Dimocarpus longana) seedlings under acid rain stress].

    Science.gov (United States)

    Liu, Jian-fu; Wang, Ming-yuan; Yang, Chen; Zhu, Ai-jun

    2013-08-01

    This paper studied the effects of exogenous nitric oxide donor sodium nitroprusside (SNP) on the chlorophyll content, antioxidant enzyme activities, and osmotic regulation substances of longan (Dimocarpus longana 'Fuyan') seedlings under acid rain (pH 3.0) stress. Under the acid rain stress, the seedling leaf superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities and chlorophyll, soluble protein and soluble sugar contents decreased obviously, while the leaf malondialdedyde content had a remarkable increase, suggesting the toxic effect of the acid rain on the seedlings. Exogenous nitric oxide had dual nature on the physiological characteristics of longan seedlings under acid rain stress. Applying 0.1-0.5 mmol x L(-1) of SNP improved the SOD, POD and CAT activities and the chlorophyll, soluble protein and soluble sugar contents significantly, and decreased the malondialdedyde content. Low concentrations SNP reduced the oxidative damage caused by the acid rain stress, and 0.5 mmol x L(-1) of SNP had the best effect. Under the application of 0.5 mmol x L(-1) of SNP, the total chlorophyll, soluble protein, and soluble sugar contents and the SOD, POD and CAT activities increased by 76.0%, 107.0%, 216.1%, 150. 0%, 350.9% and 97.1%, respectively, and the malondialdedyde content decreased by 46.4%. It was suggested that low concentration (0.1-0.5 mmol x L(-1)) SNP could alleviate the toxic effect of acid rain stress on longan seedlings via activating the leaf antioxidant enzyme activities and reducing oxidative stress, while high concentration SNP (1.0 mmol x L(-1)) lowered the mitigation effect.

  14. Homogenity of oil and sugar components of flour amaranth investigated by GC-MS

    Directory of Open Access Journals (Sweden)

    Psodorov Đorđe B.

    2015-01-01

    Full Text Available Gas chromatography with mass spectrometry (GC-MS was used for performing a qualitative analysis of liposoluble and hydrosoluble flour extracts of three genotypes of Amaranthus sp. All three samples were first defatted with hexane. Hexane extracts were used for the analysis of fatty acids of lipid components. TMSH (Trimethylsulfonium hydroxide, 0.2M in methanol was used as the transesterification reagent. With transesterification reaction, fatty acids were esterified from acilglycerol to methyl-esters. Defatted flour samples were dried in the air and then extracted with ethanol. Ethanol extracts were used for the analysis of soluble carbohydrates. TMSI (trimethylsilylimidazole was used as a reagent for the derivatization of carbohydrates into trimethylsilylethers. The results show that the dominant methyl-esters of fatty acids are very similar in all the three samples. Such a similarity was not detected in the analysis of soluble sugars. The following test cluster analysis was used for the comparison of liposoluble and hydrosoluble flour extracts of three genotypes of Amaranthus sp.

  15. 76 FR 36512 - USDA Increases the Domestic Sugar Overall Allotment Quantity, Reassigns Domestic Cane Sugar...

    Science.gov (United States)

    2011-06-22

    ... imports. The OAQ was increased due to an increase in estimated sugar demand since the FY 2011 OAQ was... sugar imports, as required by law. Upon review of the domestic sugarcane processors' sugar marketing allocations relative to their FY 2011 expected raw sugar supplies, CCC determined that all sugarcane...

  16. Damage to the microbial cell membrane during pyrolytic sugar utilization and strategies for increasing resistance.

    Science.gov (United States)

    Jin, Tao; Rover, Marjorie R; Petersen, Elspeth M; Chi, Zhanyou; Smith, Ryan G; Brown, Robert C; Wen, Zhiyou; Jarboe, Laura R

    2017-09-01

    Lignocellulosic biomass is an appealing feedstock for the production of biorenewable fuels and chemicals, and thermochemical processing is a promising method for depolymerizing it into sugars. However, trace compounds in this pyrolytic sugar syrup are inhibitory to microbial biocatalysts. This study demonstrates that hydrophobic inhibitors damage the cell membrane of ethanologenic Escherichia coli KO11+lgk. Adaptive evolution was employed to identify design strategies for improving pyrolytic sugar tolerance and utilization. Characterization of the resulting evolved strain indicates that increased resistance to the membrane-damaging effects of the pyrolytic sugars can be attributed to a glutamine to leucine mutation at position 29 of carbon storage regulator CsrA. This single amino acid change is sufficient for decreasing EPS protein production and increasing membrane integrity when exposed to pyrolytic sugars.

  17. Effect of sucrose and potassium metabisulphite on the physicochemical and microbial analysis of apricot pulp

    International Nuclear Information System (INIS)

    Ayub, M.; Durrani, T.; Zeb, A.

    2007-01-01

    Effect of different concentration of sucrose and potassium metabisulphite on the apricot pulp was studied fortnightly for 90 days through physicochemical and microbial analysis. No significant change in total soluble solids (TSS) of apricot pulp was observed during the storage. Acidity and non-reducing sugar significantly (p<0.05) decreased, while pH and reducing sugars significantly (p<0.05) increased during the storage. Samples with added 20% sucrose and 0.2% potassium metabisulphite, packed in plastic and glass containers, had negligible microbial population maintained maximum nutrients and the best sensory characteristics during the storage. Storage duration and treatments had significant (p<0.05) effect on pH, acidity, non reducing sugars and total fungal count, while on TSS (total soluble solids) and reducing sugar, the effect of treatments was nonsignificant (p<0.05). (author)

  18. Effects of Bacterial Inoculants and Absorbents on Fermentation Properties and Chemical Composition of Fresh Sugar Beet Pulp Silage Using Laboratory silos

    Directory of Open Access Journals (Sweden)

    Saeid Seidali Dolat-Abad

    2016-04-01

    Full Text Available Introduction Ensiling is one of the common preserving methods for forage or other organic materials. In this method, organic matters were preserved by proving an aerobic condition and then by reducing the pH with increasing acids production (mainly lactic acid. Some circumstances like enough soluble carbohydrates, low buffering capacity and appropriate dry matter concentration are needed in ensilages for an ideal silage production. Seepage production during ensiling is one of the most problems especially when high moisture materials (like fresh beet sugar pulp are ensiled. Silage seepage can pollute the environment and make loses in nutrients like soluble carbohydrates, protein, organic acids and etc. Moreover, lactic acid bacteria inoculants (Mainly consist of Lactobacillus plantarum have been widely used for improving fermentation pattern in ensilages. These external provided bacteria usually enhance lactic acid production in silage and then accelerate the falling of pH values in silages. Rapid decrease in pH can inhibit non-beneficial bacteria from activity which finally preserves nutrients from un-necessary fermentation or oxidation. The aim of this study was to investigate the interactive effects of lactic acid bacteria inoculants and some absorbents (straw and pith on chemical properties and fermentation profile of wet sugar beet pulp silage. Materials and Methods In the first experiment, fresh wet sugar beet pulp was treated with 5% straw or 5% pith in order to investigate the effects of these absorbents on chemical composition, fermentation characteristics and effluent production during ensiling period. In the second experiment, fresh wet sugar beet pulp was treated with a commercial lactic acid bacteria inoculants (Ecosyle and/or 5% pith in order to investing the main and interaction effects of the bacterial bacteria inoculants and the best absorbents from the experiment 1. In both experiments, triplicate samples were prepared for each

  19. Study on the Varying Patterns of Total Phospholipids, Selenium, Phosphorus, Reducing Sugar and Total Sugar, Hydrolyzed Amino Acids in the Velvet Antler of Northeast Sika Deer in Growth Period

    Directory of Open Access Journals (Sweden)

    Wang Shu-li

    2016-01-01

    Full Text Available In this study, the varying patterns of total phospholipids, selenium, phosphorus, reducing sugar and total sugar, hydrolyzed amino acids in the velvet antler of Northeast sika deer in growth period were evaluated. Eighteen Northeast sika deer were allocated into 6 groups according to the antler shedding time. Results indicated that there was significant difference of the selenium content between any two of the six groups (P<0.05 except that of Group 1 and Group 2 or Group 5 and Group 6. About the phosphorus there was significant difference between any two of the six groups (P<0.05 except that of Group 4 and Group 5 or Group 2 and Group 3 or Group 1 and Group 2. Group 6 had the lowest total Phospholipids content. Both of the reducing sugar and total sugar showed an increasing pattern initially and then decrease gradually.

  20. 75 FR 53013 - Fiscal Year 2011 Tariff-rate Quota Allocations for Raw Cane Sugar, Refined and Specialty Sugar...

    Science.gov (United States)

    2010-08-30

    ... for Raw Cane Sugar, Refined and Specialty Sugar, and Sugar-containing Products; Revision AGENCY... August 17, 2010 concerning Fiscal Year 2011 tariff-rate quota allocations of raw cane sugar, refined and special sugar, and sugar-containing products. USTR is revising the effective date of that notice to...

  1. The Prodrug Approach: A Successful Tool for Improving Drug Solubility

    Directory of Open Access Journals (Sweden)

    Daniela Hartmann Jornada

    2015-12-01

    Full Text Available Prodrug design is a widely known molecular modification strategy that aims to optimize the physicochemical and pharmacological properties of drugs to improve their solubility and pharmacokinetic features and decrease their toxicity. A lack of solubility is one of the main obstacles to drug development. This review aims to describe recent advances in the improvement of solubility via the prodrug approach. The main chemical carriers and examples of successful strategies will be discussed, highlighting the advances of this field in the last ten years.

  2. Gamma ray induced fruit quality variations in banana variety Nendran (Musa Paradasiaca L.)

    International Nuclear Information System (INIS)

    Radha Devi, D.S.; Nayer, N.K.

    1992-01-01

    Gamma ray induced fruit quality variation was envisaged to analyse the direct effect of Co 60 gamma rays in banana variety Nendran. Fruit quality analysis showed that the total soluble solids and acidity decreased and total sugar and sugar acid ratio increased with increase in dose of gamma ray exposures. (author). 5 refs., 1 tab

  3. Methods for dehydration of sugars and sugar alcohols

    Science.gov (United States)

    Holladay, Johnathan E [Kennewick, WA; Hu, Jianli [Kennewick, WA; Zhang, Xinjie [Burlington, MA; Wang, Yong [Richland, WA

    2010-08-10

    The invention includes a method of dehydration of a sugar using a dehydration catalyst and a co-catalyst within a reactor. A sugar is introduced and H.sub.2 is flowed through the reactor at a pressure of less than or equal to about 300 psig to convert at least some of the sugar into an anhydrosugar product. The invention includes a process for producing isosorbide. A starting material comprising sorbitol is flowed into a reactor. H.sub.2 is counter flowed through the reactor. The starting material is exposed to a catalyst in the presence of a co-catalyst which comprises at least one metal. The exposing is conducted at a hydrogen pressure of less than or equal to 300 psig within the reactor and the hydrogen removes at least some of any water present during the exposing and inhibits formation of colored byproducts.

  4. Active intestinal drug absorption and the solubility-permeability interplay.

    Science.gov (United States)

    Porat, Daniel; Dahan, Arik

    2018-02-15

    The solubility-permeability interplay deals with the question: what is the concomitant effect on the drug's apparent permeability when increasing the apparent solubility with a solubility-enabling formulation? The solubility and the permeability are closely related, exhibit certain interplay between them, and ongoing research throughout the past decade shows that treating the one irrespectively of the other may be insufficient. The aim of this article is to provide an overview of the current knowledge on the solubility-permeability interplay when using solubility-enabling formulations for oral lipophilic drugs, highlighting active permeability aspects. A solubility-enabling formulation may affect the permeability in opposite directions; the passive permeability may decrease as a result of the apparent solubility increase, according to the solubility-permeability tradeoff, but at the same time, certain components of the formulation may inhibit/saturate efflux transporters (when relevant), resulting in significant apparent permeability increase. In these cases, excipients with both solubilizing and e.g. P-gp inhibitory properties may lead to concomitant increase of both the solubility and the permeability. Intelligent development of such formulation will account for the simultaneous effects of the excipients' nature/concentrations on the two arms composing the overall permeability: the passive and the active arms. Overall, thorough mechanistic understanding of the various factors involved in the solubility-permeability interplay may allow developing better solubility-enabling formulations, thereby exploiting the advantages analyzed in this article, offering oral delivery solution even for BCS class IV drugs. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Trends in sugar supply and consumption in Australia: is there an Australian Paradox?

    Science.gov (United States)

    Rikkers, Wavne; Lawrence, David; Hafekost, Katherine; Mitrou, Francis; Zubrick, Stephen R

    2013-07-18

    High consumption of refined carbohydrate, in particular sugar, has been identified as a possible contributory factor in greater risk of excess weight gain. In spite of data limitations, one recent paper suggests that Australian sugar consumption has decreased over the same time period that obesity has increased, a so called 'Australian Paradox'. Given the significant public health focus on nutrition, we aimed to estimate Australian sugar supply and consumption over recent decades, to determine whether these data could be used to make any conclusions about sugar's role in obesity. Foods high in sugar were identified. Data relating to sugar supply and consumption from 1988 to 2010 were obtained from multiple sources. Using these data we attempted to generate a time series estimate of sugar in Australia's food supply. Australia produces and exports sugar from sugar cane and the sugar in imported foods has received little attention. We were unable to produce a reliable and robust estimate of total sugars in the Australian diet due to data limitations and a lack of current data sources. However, available Import data showed large increases in the volume and value of imported sweetened products between 1988 and 2010 to over 30 grams of sugar per person per day. Value estimates of local production of sweetened products also show substantial increases in this period. The Australian Paradox assertion is based on incomplete data, as it excludes sugar contained in imported processed foods, which have increased markedly. A major Australian public health target is to improve the quality of the food supply, and actions have been set in terms of achieving broader environmental changes. However, evaluation of progress is hampered by lack of high quality data relating to supply and consumption. We recommend the regular collection of comprehensive food supply statistics, which include both local production and imports. This would provide an inexpensive addition to survey data and

  6. Life Cycle Assessment of Sugar Production (VB)

    DEFF Research Database (Denmark)

    Teljigovic, Mehmed; Mengiardi, Jon; Factor, Gabriela

    1999-01-01

    The environmental organisation NOAH has proposed carrying out an environmental assessment of two different sugar productions (using sugar beet or sugar cane) in order to illustrate which of the systems has a higher environmental impact for sugar consumption in Denmark. Therefore a comparison...... will be made between sugar from sugar beet produced in Denmark versus sugar produces from sugar cane in a tropical country, Brazil, and transported afterwards to Denmark. To evaluate the environmental aspects of these two product systems a Life Cycle Assessement (LCA) will be carried out.From the results...... obtained in the present LCA of sugar produces from sugar canes or sugar beet it is difficult to make an immediate choice between the two possibilities. Indeed, Quantitative results from the EDIP (Environmental Design of Industrial Products) software are globally similar for both ways of producing sugar...

  7. Substitution of Sugar with Dates Powder and Dates Syrup in Cookies Making

    OpenAIRE

    W.A. Alsenaien; R.A. Alamer; Zhen-Xing Tang; S.A. Albahrani; M.A. Al-Ghannam; S.M. Aleid

    2015-01-01

    In this study, the effects of dates powder and dates syrup as a sugar substitution, on the physical properties and sensory attributes of cookies were studied. An increase in firmness and moisture content of cookies supplemented with dates was obtained. The diameter and spread ratio of cookies showed a decrease with increasing levels of date powder or date syrup. Partial replacement of sugar with date powder or date syrup produced cookies with more dark and red color. Sensory evaluation result...

  8. Effect of timing and intensity of defoliation on yield and quality of sugar beet

    Directory of Open Access Journals (Sweden)

    ali kamandi

    2009-06-01

    Full Text Available The effect of defoliation on root yield and quality of suagrbeet (Beta vulgaris L. was examined in a field study in Research Station of Faculty of Agriculture, Ferdowsi University of Mashhd during 2005 growing season. The experiment was conducted as a split plot on the basis of randomized complete block design with three replications.Time of Defoliation (May 16th, May 31th, June 18th, July 9th, July 31th, and August 20th were allocated to main plots and defoliation severity (0, 30, 60, and 100% were assigned to subplots. Results showed that root yield, shoot and root dry weight and sugar yield were influenced by defoliation time. However, defoliation time had no significant effects on sugar white content and white sugar yield. Effects of defoliation on plant growth was more pronounced in mid season when about 1500 degree days was accumulated, compared to early or late season. Defoliation intensity resulted in a significant decrease in root yield, shoot fresh weigth, root and shoot dry weight, sugar and white sugar content, and white sugar yield compared with undefoliated control, but molass sugar was increased in all defoliation intensities. sugar and white suagr percent were significantly affected by defoliation time x intensity interaction. Complete defoliation (100% on July 30th and Auguat 19th led to 18 and 21% reduction in sugar and 29 and 28% in white suagar, respectively. However, 100% defoliation in July 8th resulted in 6 and 8% increase in sugar and white sugar, respectively. Keywords: Time of defoliation, defoliation intensity, sugarbeet yield,sugar beet quality.

  9. Changes in consumption of added sugars from age 13 to 30 years: a systematic review and meta-analysis of longitudinal studies.

    Science.gov (United States)

    Winpenny, E M; Penney, T L; Corder, K; White, M; van Sluijs, E M F

    2017-11-01

    Added sugar intake during adolescence has been associated with weight gain and cardiometabolic risk factors. Moreover, dietary habits may persist into adulthood, increasing chronic disease risk in later life. This systematic review investigated changes in intake of added sugars between the ages of 13 and 30 years. Literature databases were searched for longitudinal studies of diet during adolescence or early adulthood. Retrieved articles were screened for studies including multiple measures of intake of sugars or sugary foods from cohort participants between the ages of 13 and 30. Data were analysed using random-effects meta-analysis, by the three main nutrient and food group categories identified (PROSPERO: CRD42015030126). Twenty-four papers reported longitudinal data on intake of added sugar or sucrose (n = 6), sugar-sweetened beverages (SSBs) (n = 20) and/or confectionery (n = 9). Meta-analysis showed a non-significant per year of age decrease in added sugar or sucrose intake (-0.15% total energy intake (95%CI -0.41; 0.12)), a decrease in confectionery consumption (-0.20 servings/week (95%CI -0.41; -0.001)) and a non-significant decrease in SSB consumption (-0.15 servings/week (95%CI -0.32; 0.02)). Taken together, the overall decrease in added sugar intake observed from adolescence to early adulthood may suggest opportunities for intervention to further improve dietary choices within this age range. © 2017 The Authors. Obesity Reviews published by John Wiley & Sons Ltd on behalf of World Obesity Federation.

  10. Solubility of Tc(IV) oxides

    International Nuclear Information System (INIS)

    Liu, D.J.; Fan, X.H.

    2005-01-01

    Full text of publication follows: The deep geological disposal of the high level radioactive wastes is expected to be a safer disposal method in most countries. The long-lived fission product 99 Tc is present in large quantities in nuclear wastes and its chemical behavior in aqueous solution is of considerable interest. Under the reducing conditions, expected to exist in a deep geological repository, it is generally predicted that technetium will be present as TcO 2 .nH 2 O. The solubility of Tc(IV) is used as a source term in performance assessment of radioactive waste repository. Technetium oxide was prepared by reduction of a technetate solution with Sn 2+ . The solubility of Tc(IV) oxide has been determined in simulated groundwater and re-distilled water under aerobic and anaerobic conditions. The effects of pH and CO 3 2- concentration of solution on solubility of Tc(IV) oxide were studied. The concentration of total technetium and Tc(IV) species in the solutions were periodically determined by separating the oxidized and reduced technetium species using a solvent extraction procedure and counting the beta activity of the 99 Tc with a liquid scintillation counter. The experimental results show that the rate of oxidation of Tc(IV) in simulated groundwater and re-distilled water is about (1.49∼1.86) x 10 -9 mol/(L.d) under aerobic conditions, but Tc(IV) in simulated groundwater and re-distilled water is not oxidized under anaerobic conditions. Under aerobic or anaerobic conditions the solubility of Tc(IV) oxide in simulated groundwater and re-distilled water is equal on the whole after centrifugation or ultrafiltration. The solubility of Tc(IV) oxide decreases with the increase of pH at pH 10 and is pH independent in the range 2 -8 to 10 -9 mol/L at 2 3 2- concentration. These data could be used to estimate the Tc(IV) solubility for cases where solubility limits transport of technetium in reducing environments of high-level waste repositories. (authors)

  11. Closing the Yield Gap of Sugar Beet in the Netherlands-A Joint Effort.

    Science.gov (United States)

    Hanse, Bram; Tijink, Frans G J; Maassen, Jurgen; van Swaaij, Noud

    2018-01-01

    The reform of the European Union's sugar regime caused potential decreasing beet prices. Therefore, the Speeding Up Sugar Yield (SUSY) project was initiated. At the start, a 3 × 15 target was formulated: in 2015 the national average sugar yield in the Netherlands equals 15 t/ha (60% of the sugar beet potential) and the total variable costs 15 euro/t sugar beet, aspiring a saving on total variable costs and a strong increase in sugar yield. Based on their average sugar yield in 2000-2004, 26 pairs of "type top" (high yielding) and "type average" (average yielding) growers were selected from all sugar beet growing regions in the Netherlands. On the fields of those farmers, all measures of sugar beet cultivation were investigated, including cost calculation and recording phytopathological, agronomical and soil characteristics in 2006 and 2007. Although there was no significant difference in total variable costs, the "type top" growers yielded significantly 20% more sugar in each year compared to the "type average" growers. Therefore, the most profitable strategy for the growers is maximizing sugar yield and optimizing costs. The difference in sugar yield between growers could be explained by pests and diseases (50%), weed control (30%), soil structure (25%) and sowing date (14%), all interacting with each other. The SUSY-project revealed the effect of the grower's management on sugar yield. As a follow up for the SUSY-project, a growers' guide "Suikerbietsignalen" was published, Best Practice study groups of growers were formed and trainings and workshops were given and field days organized. Further, the benchmarking and feedback on the crop management recordings and the extension on variety choice, sowing performance, foliar fungi control and harvest losses were intensified. On the research part, a resistance breaking strain of the Beet Necrotic Yellow Vein Virus (BNYVV) and a new foliar fungus, Stemphylium beticola , were identified and options for control were

  12. Purification and characterization of a soluble glycoprotein from garlic (Allium sativum) and its in vitro bioactivity.

    Science.gov (United States)

    Wang, Yan; Zou, Tingting; Xiang, Minghui; Jin, Chenzhong; Zhang, Xuejiao; Chen, Yong; Jiang, Qiuqing; Hu, Yihong

    2016-10-02

    A soluble glycoprotein was purified to homogeneity from ripe garlic (Allium sativum) bulbs using ammonium sulfate precipitation, Sephadex G-100 gel filtration, and diethylaminoethyl-52 cellulose anion-exchange chromatography. A native mass of 55.7 kDa estimated on gel permeation chromatography and a molecular weight of 13.2 kDa observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis supported that the glycoprotein is a homotetramer. β-Elimination reaction result suggested that the glycoprotein is an N-linked type. Fourier-transform infrared spectroscopy proved that it contains sugar. Gas chromatography-mass spectrometer analysis showed that its sugar component was galactose. The glycoprotein has 1,1-diphenyl-2-picrylhydrazil free radical scavenging activity and the peroxidation inhibition ability to polyunsaturated fatty acid. These results indicated that the glycoprotein has potential for food additives, functional foods, and even biotechnological and medical applications.

  13. Generation of Soluble Advanced Glycation End Products Receptor (sRAGE)-Binding Ligands during Extensive Heat Treatment of Whey Protein/Lactose Mixtures Is Dependent on Glycation and Aggregation

    NARCIS (Netherlands)

    Liu, Fahui; Teodorowicz, Gosia; Wichers, Harry J.; Boekel, van Tiny; Hettinga, Kasper A.

    2016-01-01

    Heating of protein- and sugar-containing materials is considered the primary factor affecting the formation of advanced glycation end products (AGEs). This study aimed to investigate the influence of heating conditions, digestion, and aggregation on the binding capacity of AGEs to the soluble AGE

  14. A trial of sugar-free or sugar-sweetened beverages and body weight in children

    NARCIS (Netherlands)

    de Ruyter, Janne C; Olthof, Margreet R; Seidell, Jacob C; Katan, Martijn B

    2012-01-01

    BACKGROUND: The consumption of beverages that contain sugar is associated with overweight, possibly because liquid sugars do not lead to a sense of satiety, so the consumption of other foods is not reduced. However, data are lacking to show that the replacement of sugar-containing beverages with

  15. Improving conversion yield of fermentable sugars into fuel ethanol in 1st generation yeast-based production processes.

    Science.gov (United States)

    Gombert, Andreas K; van Maris, Antonius J A

    2015-06-01

    Current fuel ethanol production using yeasts and starch or sucrose-based feedstocks is referred to as 1st generation (1G) ethanol production. These processes are characterized by the high contribution of sugar prices to the final production costs, by high production volumes, and by low profit margins. In this context, small improvements in the ethanol yield on sugars have a large impact on process economy. Three types of strategies used to achieve this goal are discussed: engineering free-energy conservation, engineering redox-metabolism, and decreasing sugar losses in the process. Whereas the two former strategies lead to decreased biomass and/or glycerol formation, the latter requires increased process and/or yeast robustness. Copyright © 2014 Elsevier Ltd. All rights reserved.

  16. Mechanisms for oral absorption of poorly water-soluble compounds

    DEFF Research Database (Denmark)

    Lind, Marianne Ladegaard

    Abstract A large part of the new drug candidates discovered by the pharmaceutical industry have poor solubility in aqueous media. The preferred route of drug administration is the oral route, but for these poorly water-soluble drug candidates the oral bioavailability can be low and variable. Often......, phospholipids) and exogenous surfactants used in pharmaceutical formulations on the oral absorption of poorly water-soluble drug substances. Three different models were used for this purpose. The first model was the in vitro Caco-2 cell model. Simulated intestinal fluids which did not decrease cellular...... products are important for the solubilization of poorly water-soluble drug substances and thus absorption. The second model used was the lipoprotein secreting Caco-2 cell model, which was used to simulate intestinal lymphatic transport in vitro. Various simulated intestinal fluids were composed...

  17. Sugar holograms with erioglaucine and tartrazine

    Science.gov (United States)

    Mejias-Brizuela, N. Y.; Olivares-Pérez, A.; Páez-Trujillo, G.; Fuentes-Tapia, I.

    2007-09-01

    An artificial green colorant, composed by erioglaucine (Blue 1) and tartrazine (Yellow 5), was employed in a sugar matrix to improve the material sensibility and to make a comparative analysis of the diffraction efficiency parameter, for holograms replications, the holographic pattern was obtained by a computer and recorded in sugar films and in modified sugar (sugar-colorant). Conventional lithography and UV radiation were used. The results show that the behavior diffraction efficiency of the sugar-colorant films is slightly larger than in the sugar matrix under the same recording conditions.

  18. Identification of candidate genes involved in the sugar metabolism and accumulation during pear fruit post-harvest ripening of 'Red Clapp's Favorite' (Pyrus communis L.) by transcriptome analysis.

    Science.gov (United States)

    Wang, Long; Chen, Yun; Wang, Suke; Xue, Huabai; Su, Yanli; Yang, Jian; Li, Xiugen

    2018-01-01

    Pear ( Pyrus spp.) is a popular fruit that is commercially cultivated in most temperate regions. In fruits, sugar metabolism and accumulation are important factors for fruit organoleptic quality. Post-harvest ripening is a special feature of 'Red Clapp's Favorite'. In this study, transcriptome sequencing based on the Illumina platform generated 23.8 - 35.8 million unigenes of nine cDNA libraries constructed using RNAs from the 'Red Clapp's Favorite' pear variety with different treatments, in which 2629 new genes were discovered, and 2121 of them were annotated. A total of 2146 DEGs, 3650 DEGs, 1830 DEGs from each comparison were assembled. Moreover, the gene expression patterns of 8 unigenes related to sugar metabolism revealed by qPCR. The main constituents of soluble sugars were fructose and glucose after pear fruit post-harvest ripening, and five unigenes involved in sugar metabolism were discovered. Our study not only provides a large-scale assessment of transcriptome resources of 'Red Clapp's Favorite' but also lays the foundation for further research into genes correlated with sugar metabolism.

  19. Effect of biosurfactants on the aqueous solubility of PCE and TCE.

    Science.gov (United States)

    Albino, John D; Nambi, Indumathi M

    2009-12-01

    The effect of biosurfactants on the solubility of tetrachloroethylene (PCE) and trichloroethylene (TCE) was studied in batch experiments pertaining to their use for solubilization and mobilization of such contaminants in surfactant enhanced aquifer remediation. Biosurfactants, rhamnolipid and surfactin used in solubility studies were synthesized in our laboratory by Pseudomonas aeruginosa (MTCC 2297) and Bacillus subtilis (MTCC 2423), respectively. The efficiency of the biosurfactants in solubilizing the chlorinated solvents was compared to that of synthetic surfactants. The Weight Solubilization Ratio (WSR) values for solubilization of PCE and TCE by biosurfactants were very high compared to the values obtained for synthetic surfactants. Surfactin proved to be a better surfactant over rhamnolipid. The WSR of surfactin on solubilization of PCE and TCE were 3.83 and 12.5, respectively, whereas the values obtained for rhamnolipid were 2.06 and 8.36. The solubility of the chlorinated solvents by biosurfactants was considerably affected by the changes in pH. The aqueous solubility of PCE and TCE increased tremendously with decrease in pH. The solubility of biosurfactants was observed to decrease with the pH, favoring partitioning of surfactants into the chlorinated solvents in significant amounts at lower pH. The excessive accumulation of biosurfactants at the interface facilitated interfacial tension reductions resulting in higher solubility of the chlorinated solvents at pH less than 7.

  20. 14C-incorporation into sugars and organic acids of water-stressed maize leaves

    International Nuclear Information System (INIS)

    Becker, T.; Fock, H.

    1986-01-01

    The incorporation of 14 C into sugars and some organic acids of maize leaves has been studied in relation to the leaf water potential by feeding 14 CO 2 (370 ppm) for 1,2 and 4 min during steady state photosynthesis at 25 0 C (PAR = 800 μmol m -2 s -1 ). The relative specific radioactivity (RSA) of the sugars was low (0.2% after 4 min) at -0.62 MPa (control) and decreased by about 50% when psi dropped to -0.95 MPa. The authors conclude that the low rate of photosynthetic sugar synthesis in maize leaves decreased during water stress. The RSA of malate was extremely low at -0.62 MPa (0.02%). This result may be the consequence of the large pool size of malate in maize leaves. The authors presume that there are two malate pools present in maize leaves, a small metabolic pool and a larger storage pool. The RSA of malate decreased during the stress period. This is consistent with the decline in net CO 2 uptake during water stress. The pool sizes of citrate and isocitrate increased when psi dropped to -0.95 MPa. As practically no radioactivity was detected in these organic acids, they conclude that these compounds are synthesized from unlabelled precursors during water stress

  1. Solubility of ferrocyanide compounds. Ferrocyanide Safety Project, Interim report FY1994

    International Nuclear Information System (INIS)

    Rai, D.; Felmy, A.R.; Smith, S.C.; Ryan, J.L.

    1994-10-01

    The solubility of Cs 2 NiFe(CN) 6 (c) [1] as a function of NaOH and temperature was determined to ascertain whether [1] shows retrograde solubility (i.e., decreasing solubility with increasing temperature), which would have bearing on the possible formation of ''hot spots'' in the tanks and thus the safety of the ferrocyanide tanks. The results show that the aqueous concentrations of Cs in equilibrium with [1] at 25, 60, 75 and 90 C are similar (within the limits of experimental error), indicating that [1] does not show retrograde solubility. To understand general solubility relationships of Ni 2 Fe(CN) 6 (c) [2] and to determine the influence on solubility of high electrolyte concentrations (e.g., NaNO 3 ) that are commonly encountered in the ferrocyanide tanks, the solubility of [2] as a function of CsNO 3 , NiCl 2 , and NaNO 3 was determined. In general, [2] is fairly insoluble and shows slightly increased solubility at high electrolyte concentrations only. For [2] in NiCl 2 , the aqueous Fe concentrations show first a decrease and then an increase with the increase in NiCl 2 concentrations. The increase in Fe concentrations at high Ni concentrations appears to be the result of replacement of Fe by Ni in the [2] structure. For [2] in CsNO 3 and at 0.001 M Na 4 Fe(CN) 6 , the Cs is quantitatively removed from solution at low added Cs concentrations and appears to approach the final solid composition of [1]. The solubility of [2] in NaNO 3 and at 0.001 M Na 4 Fe(CN) 6 shows an increase in Ni concentrations to about 0.5 mg/l at NaNO 3 concentrations > 1.0 M. These increased Ni concentrations may be the result of substitution of Na for Ni in the solid phase

  2. Impact of earthworms on trace element solubility in contaminated mine soils amended with green waste compost

    Energy Technology Data Exchange (ETDEWEB)

    Sizmur, Tom, E-mail: t.p.sizmur@reading.ac.uk [Soil Research Centre, Dept. Geography and Environmental Science, School of Human and Environmental Sciences, University of Reading, Whiteknights, Reading, RG6 6DW (United Kingdom); Palumbo-Roe, Barbara [British Geological Survey, Kingsley Dunham Centre, Keyworth, Nottingham, NG12 5GG (United Kingdom); Hodson, Mark E. [Soil Research Centre, Dept. Geography and Environmental Science, School of Human and Environmental Sciences, University of Reading, Whiteknights, Reading, RG6 6DW (United Kingdom)

    2011-07-15

    The common practice of remediating metal contaminated mine soils with compost can reduce metal mobility and promote revegetation, but the effect of introduced or colonising earthworms on metal solubility is largely unknown. We amended soils from an As/Cu (1150 mgAs kg{sup -1} and 362 mgCu kg{sup -1}) and Pb/Zn mine (4550 mgPb kg{sup -1} and 908 mgZn kg{sup -1}) with 0, 5, 10, 15 and 20% compost and then introduced Lumbricus terrestris. Porewater was sampled and soil extracted with water to determine trace element solubility, pH and soluble organic carbon. Compost reduced Cu, Pb and Zn, but increased As solubility. Earthworms decreased water soluble Cu and As but increased Pb and Zn in porewater. The effect of the earthworms decreased with increasing compost amendment. The impact of the compost and the earthworms on metal solubility is explained by their effect on pH and soluble organic carbon and the environmental chemistry of each element. - Graphical abstract: Display Omitted Highlights: > Compost reduced the mobility of Cu, Pb and Zn. > Compost increased the mobility of As. > Earthworms decreased water soluble As and Cu but increased Pb and Zn in porewater. > These effects are explained by the impact of the earthworms and compost on pH and DOC. - The effect of earthworms on metal solubility was due to changes in dissolved organic carbon and pH but was reduced with increasing compost amendments.

  3. Impact of earthworms on trace element solubility in contaminated mine soils amended with green waste compost

    International Nuclear Information System (INIS)

    Sizmur, Tom; Palumbo-Roe, Barbara; Hodson, Mark E.

    2011-01-01

    The common practice of remediating metal contaminated mine soils with compost can reduce metal mobility and promote revegetation, but the effect of introduced or colonising earthworms on metal solubility is largely unknown. We amended soils from an As/Cu (1150 mgAs kg -1 and 362 mgCu kg -1 ) and Pb/Zn mine (4550 mgPb kg -1 and 908 mgZn kg -1 ) with 0, 5, 10, 15 and 20% compost and then introduced Lumbricus terrestris. Porewater was sampled and soil extracted with water to determine trace element solubility, pH and soluble organic carbon. Compost reduced Cu, Pb and Zn, but increased As solubility. Earthworms decreased water soluble Cu and As but increased Pb and Zn in porewater. The effect of the earthworms decreased with increasing compost amendment. The impact of the compost and the earthworms on metal solubility is explained by their effect on pH and soluble organic carbon and the environmental chemistry of each element. - Graphical abstract: Display Omitted Highlights: → Compost reduced the mobility of Cu, Pb and Zn. → Compost increased the mobility of As. → Earthworms decreased water soluble As and Cu but increased Pb and Zn in porewater. → These effects are explained by the impact of the earthworms and compost on pH and DOC. - The effect of earthworms on metal solubility was due to changes in dissolved organic carbon and pH but was reduced with increasing compost amendments.

  4. DEPENDENCY OF SULFATE SOLUBILITY ON MELT COMPOSITION AND MELT POLYMERIZATION

    International Nuclear Information System (INIS)

    JANTZEN, CAROL M.

    2004-01-01

    Sulfate and sulfate salts are not very soluble in borosilicate waste glass. When sulfate is present in excess it can form water soluble secondary phases and/or a molten salt layer (gall) on the melt pool surface which is purported to cause steam explosions in slurry fed melters. Therefore, sulfate can impact glass durability while formation of a molten salt layer on the melt pool can impact processing. Sulfate solubility has been shown to be compositionally dependent in various studies, (e.g. , B2O3, Li2O, CaO, MgO, Na2O, and Fe2O3 were shown to increase sulfate solubility while Al2O3 and SiO2 decreased sulfate solubility). This compositional dependency is shown to be related to the calculated melt viscosity at various temperatures and hence the melt polymerization

  5. Source-to-sink transport of sugar and regulation by environmental factors.

    Science.gov (United States)

    Lemoine, Remi; La Camera, Sylvain; Atanassova, Rossitza; Dédaldéchamp, Fabienne; Allario, Thierry; Pourtau, Nathalie; Bonnemain, Jean-Louis; Laloi, Maryse; Coutos-Thévenot, Pierre; Maurousset, Laurence; Faucher, Mireille; Girousse, Christine; Lemonnier, Pauline; Parrilla, Jonathan; Durand, Mickael

    2013-01-01

    Source-to-sink transport of sugar is one of the major determinants of plant growth and relies on the efficient and controlled distribution of sucrose (and some other sugars such as raffinose and polyols) across plant organs through the phloem. However, sugar transport through the phloem can be affected by many environmental factors that alter source/sink relationships. In this paper, we summarize current knowledge about the phloem transport mechanisms and review the effects of several abiotic (water and salt stress, mineral deficiency, CO2, light, temperature, air, and soil pollutants) and biotic (mutualistic and pathogenic microbes, viruses, aphids, and parasitic plants) factors. Concerning abiotic constraints, alteration of the distribution of sugar among sinks is often reported, with some sinks as roots favored in case of mineral deficiency. Many of these constraints impair the transport function of the phloem but the exact mechanisms are far from being completely known. Phloem integrity can be disrupted (e.g., by callose deposition) and under certain conditions, phloem transport is affected, earlier than photosynthesis. Photosynthesis inhibition could result from the increase in sugar concentration due to phloem transport decrease. Biotic interactions (aphids, fungi, viruses…) also affect crop plant productivity. Recent breakthroughs have identified some of the sugar transporters involved in these interactions on the host and pathogen sides. The different data are discussed in relation to the phloem transport pathways. When possible, the link with current knowledge on the pathways at the molecular level will be highlighted.

  6. Iron solubility in highly boron-doped silicon

    International Nuclear Information System (INIS)

    McHugo, S.A.; McDonald, R.J.; Smith, A.R.; Hurley, D.L.; Weber, E.R.

    1998-01-01

    We have directly measured the solubility of iron in high and low boron-doped silicon using instrumental neutron activation analysis. Iron solubilities were measured at 800, 900, 1000, and 1100thinsp degree C in silicon doped with either 1.5x10 19 or 6.5x10 14 thinspboronthinspatoms/cm 3 . We have measured a greater iron solubility in high boron-doped silicon as compared to low boron-doped silicon, however, the degree of enhancement is lower than anticipated at temperatures >800thinsp degree C. The decreased enhancement is explained by a shift in the iron donor energy level towards the valence band at elevated temperatures. Based on this data, we have calculated the position of the iron donor level in the silicon band gap at elevated temperatures. We incorporate the iron energy level shift in calculations of iron solubility in silicon over a wide range of temperatures and boron-doping levels, providing a means to accurately predict iron segregation between high and low boron-doped silicon. copyright 1998 American Institute of Physics

  7. Solubility of ocular therapeutic agents in self-emulsifying oils. I. Self-emulsifying oils for ocular drug delivery: solubility of indomethacin, aciclovir and hydrocortisone.

    Science.gov (United States)

    Czajkowska-Kośnik, Anna; Sznitowska, Małgorzata

    2009-01-01

    Self-emulsifying drug delivery systems (SEDDS) were prepared by dissolving Cremophor EL, Tween 20, Tween 80 and Span 80 (1% or 5%) in oils (Miglyol 812 or castor oil). Solubilities of three ophthalmic drugs, namely aciclovir, hydrocortisone and indomethacin were determined in these systems. In addition, the effect of a small amount of water (0.5% and 2%) on solubilization properties of the systems was estimated. Of the three substances, indomethacin showed the best solubility in Miglyol while aciclovir was practically insoluble in this oil. The surfactants usually increased drug solubility in the oily phase. Only Tween 20 was found to decrease the solubility of aciclovir and hydrocortisone in Miglyol. Addition of a small amount of water to the oil/surfactant system increased solubility of hydrocortisone, but not of indomethacin. The results of the current study may be utilized to design a suitable composition of SEDDS and allow continuation of research on this type of drug carriers.

  8. Declining consumption of added sugars and sugar-sweetened beverages in Australia: a challenge for obesity prevention.

    Science.gov (United States)

    Brand-Miller, Jennie C; Barclay, Alan W

    2017-04-01

    Background: Reduced intakes of added sugars and sugar-sweetened beverages (SSBs) have been the main focus of efforts to stall obesity. Although obesity has risen steeply in Australia, some evidence suggests that added-sugars and SSB intakes have declined over the same time frame. Objective: We investigated recent trends in the availability of sugars and sweeteners and changes in intakes of total sugars, added sugars, and SSBs in Australia by using multiple, independent data sources. Design: The study was designed to compare relevant data published by the Food and Agriculture Organization of the United Nations [FAO Statistics Division Database (FAOSTAT)], the Australian government, academia, and the food industry. Results: With the use of the FAOSTAT food balance sheets for Australia, the per capita availability of added or refined sugars and sweeteners was shown to have fallen 16% from 152 g/d in 1980 to 127 g/d in 2011 ( P -trend = 0.001). In national dietary surveys in 1995 and 2011-2012, added-sugars intake declined markedly in adult men (from 72 to 59 g/d; -18%) but not in women (44-42 g/d; NS). As a proportion of total energy, added-sugars intake fell 10% in adult men but nonsignificantly in adult women. Between 1995 and 2011-2012, the proportion of energy from SSBs (including 100% juice) declined 10% in adult men and 20% in women. More marked changes were observed in children aged 2-18 y. Data from national grocery sales indicated that per capita added-sugars intakes derived from carbonated soft drinks fell 26% between 1997 and 2011 (from 23 to 17 g/d) with similar trends for noncarbonated beverages. Conclusions: In Australia, 4 independent data sets confirmed shorter- and longer-term declines in the availability and intake of added sugars, including those contributed by SSBs. The findings challenge the widespread belief that energy from added sugars or sugars in solution are uniquely linked to the prevalence of obesity. © 2017 American Society for Nutrition.

  9. Carbohydrates, Sugar, and Your Child

    Science.gov (United States)

    ... carbs are: simple carbohydrates (or simple sugars): including fructose, glucose, and lactose, which also are found in nutritious ... sugar, check the ingredients list for sugar, corn syrup, or other sweeteners, such as dextrose, fructose, honey, or molasses, to name just a few. ...

  10. Distribution and harmfulness of field dodder (Cuscuta campestris Yuncker at sugar beet fields in Slovakia

    Directory of Open Access Journals (Sweden)

    Tóth Peter

    2006-01-01

    Full Text Available During 2002-2004, field surveys of field dodder (Cuscuta campestris Yunck e r in croplands were done in southwestern Slovakia. From among 150 localities surveyed, 80 were found infested by the field dodder. Within crop plants, C. campestris infested sugar beet (Beta vulgaris, alfalfa (Medicago sativa tobacco (Nicotiana tabacum, potato (Solanum tuberosum, lentil (Lens esculenta, parsley (Pastinaca sativa and onion (Allium cepa. Besides the crops, 18 weed species were also recorded. The species from the genus Polygonum (Polygonaceae were the most important and acted as a significant reservoir of field dodder in cropland. C. campestris was not found in cold climatic regions with altitude higher than 240 m. The impact of field dodder infestation on sugar beet yield was studied during the year of 2004 in two localities (Šalov and Žitavce in southwestern Slovakia. The presence of field dodder markedly reduced both, quantity and quality of sugar beet yield. Weight of heavily infested beets was reduced from 21.6 to 37.4% and sugar content from 12.0 to 15.2%. Such decline of both parameters was also recorded when field dodder was removed together with leaves of sugar beet during growing season at the end of July. The aim of the infested leaves removal was to decrease mass of field dodder seeds. Although the leaf area of sugar beet regenerates, the decrease of quality and quantity was observed. The decline was the same at both localities, no matter whether the fields were irrigated (Šalov or not (Žitavce.

  11. Biochemical and histological characterization of Agave fourcroydes Lem. plants in vitro acclimatized

    Directory of Open Access Journals (Sweden)

    Yunel Pérez Hernández

    2016-10-01

    Full Text Available Henequen (Agave fourcroydes Lem. is a representative crop of the province of Matanzas, Cuba, which has been exploited extensively for the quality of its fibers. The present work aimed to characterize from the biochemical and histological point of view henequen plants cv. 'Sac Ki' in vitro acclimatized. The biochemical indicators analyzed were concentration of soluble phenols, total soluble carbohydrates, reducing sugars, soluble proteins and total chlorophylls, as well as the relative contents of flavonoids, terpins and anthocyanins. In addition, a histological analysis of the epidermis was performed and the results were compared with plants of one month in rooting culture medium. In vitro acclimatized plants were subsequently transferred to the ex vitro conditions. The contents of total soluble carbohydrates, reducing sugars and chlorophylls decreased, whereas the levels of soluble phenols, proteins and secondary metabolites were higher in relation to in vitro plants rooted for 30 days. The epidermis of the in vitro acclimatized plants showed a greater development of the stomatal apparatus that could positively affect the ex vitro acclimatization, where a survival rate of 87% and a reduction of the acclimatization time were obtained.   Keywords: chlorophyll, henequen, phenol, reducing sugar

  12. Short-Term Exposure to Electromagnetic Fields Generated by Mobile Phone Jammers Decreases the Fasting Blood Sugar in Adult Male Rats.

    Science.gov (United States)

    Shekoohi Shooli, F; Mortazavi, S A R; Jarideh, S; Nematollahii, S; Yousefi, F; Haghani, M; Mortazavi, S M J; Shojaei-Fard, M B

    2016-03-01

    Substantial evidence indicates that exposure to electromagnetic fields (EMF) above certain levels can affect human health through triggering some biological responses. According to WHO, short-term exposure to EMF at the levels present in the home/environment do not cause any apparent detrimental effects in healthy individuals. However, now, there is a debate on whether long-term exposure to low level EMF can evoke detrimental biological responses. Although based on the Communications Act of 1934, selling, advertising, using, or importing mobile jammers which block cell phone calls and text messages are illegal acts, in some countries these devices are being used for security purpose and for prevention of cheating during examinations. In this study 30 male Wistar rats were randomly divided into 3 groups of 10 each. The control group received no radiation. The sham exposure group was exposed to a switched-off jammer device. After fasting for 12 hours, the exposure group was exposed to EMFs at a distance of 50 cm from the jammer. Blood samples were collected from the tail vein after 24, 48 and72 hours and fasting blood sugar was measured by using a common blood glucose monitor (BIONIME GM110, Taiwan). The significance level was considered 5% and SPSS Ver. 21 was used for statistical analysis. The data were analyzed by ANOVA followed by Tukey's test. A statistically significant difference was observed between blood sugar level in the control and exposure groups after 24, 48 and 72 hours of continuous irradiation (p values were mobile phone jammer can reduce blood sugar level in adult male rats. These findings, in contrast with our previous results, lead us to this conclusion that the use of these signal blocking devices in very specific circumstances may have some therapeutic effects. However, further studies have to be performed to find out the exact mechanism by which Jammer EMFs reduce fasting blood sugar.

  13. The Effects of Ribose on Mechanical and Physicochemical Properties of Cold Water Fish Gelatin Films

    Directory of Open Access Journals (Sweden)

    Neda Javadian

    2014-06-01

    Full Text Available Native fish gelatin has some disadvantages such as high hydrophilic, and solubility in cold water. Mixing with other biopolymers and crosslinking by sugars may improve functional properties of fish gelatin. So in this research, the effects of ribose were investigated on moisture sorption isotherm, solubility in water, and mechanical properties of cold water fish gelatin (CWFG films. Ribose sugar was incorporated into CWFG solutions at different concentrations (e.g. 0, 2, 4, and 6% w/w dried gelatin. Physicochemical properties such as water solubility, moisture sorption isotherm and mechanical properties of the films were measured according to ASTM standards. Results showed that incorporation of ribose sugar significantly improved functional properties of CWFG films. Solubility, moisture content and monolayer water content of the matrixes were decreased by increasing the ribose contents. Mechanical properties of biocomposites were improved more than 20% and moisture sorption isotherm curve significantly shifted to lower moisture contents. The results of this study could be explored for commercial use, depending on industrial needs for either production of edible films or for packaging purposes.

  14. Rapid and prodium iodide-compatible optical clearing method for brain tissue based on sugar/sugar-alcohol

    Science.gov (United States)

    Yu, Tingting; Qi, Yisong; Wang, Jianru; Feng, Wei; Xu, Jianyi; Zhu, Jingtan; Yao, Yingtao; Gong, Hui; Luo, Qingming; Zhu, Dan

    2016-08-01

    The developed optical clearing methods show great potential for imaging of large-volume tissues, but these methods present some nonnegligible limitations such as complexity of implementation and long incubation times. In this study, we tried to screen out rapid optical clearing agents by means of molecular dynamical simulation and experimental demonstration. According to the optical clearing potential of sugar and sugar-alcohol, we further evaluated the improvement in the optical clearing efficacy of mouse brain samples, imaging depth, fluorescence preservation, and linear deformation. The results showed that drops of sorbitol, sucrose, and fructose could quickly make the mouse brain sample transparent within 1 to 2 min, and induce about threefold enhancement in imaging depth. The former two could evidently enhance the fluorescence intensity of green fluorescent protein (GFP) and prodium iodide (PI) nuclear dye. Fructose could significantly increase the fluorescence intensity of PI, but slightly decrease the fluorescence intensity of GFP. Even though the three agents caused some shrinkage in samples, the contraction in horizontal and longitudinal directions are almost the same.

  15. 75 FR 22095 - USDA Reassigns Domestic Cane Sugar Allotments and Increases the Fiscal Year 2010 Raw Sugar Tariff...

    Science.gov (United States)

    2010-04-27

    ... USDA Reassigns Domestic Cane Sugar Allotments and Increases the Fiscal Year 2010 Raw Sugar Tariff-Rate... announced a reassignment of surplus sugar under domestic cane sugar allotments of 200,000 short tons raw value (STRV) to imports, and increased the fiscal year (FY) 2010 raw sugar tariff-rate quota (TRQ) by...

  16. 75 FR 38764 - USDA Reassigns Domestic Cane Sugar Allotments and Increases the Fiscal Year 2010 Raw Sugar Tariff...

    Science.gov (United States)

    2010-07-06

    ... USDA Reassigns Domestic Cane Sugar Allotments and Increases the Fiscal Year 2010 Raw Sugar Tariff-Rate... announced a reassignment of surplus sugar under domestic cane sugar allotments of 300,000 short tons raw value (STRV) to imports, and increased the fiscal year (FY) 2010 raw sugar tariff-rate quota (TRQ) by...

  17. 76 FR 20305 - USDA Reassigns Domestic Cane Sugar Allotments and Increases the Fiscal Year 2011 Raw Sugar Tariff...

    Science.gov (United States)

    2011-04-12

    ... USDA Reassigns Domestic Cane Sugar Allotments and Increases the Fiscal Year 2011 Raw Sugar Tariff-Rate... announced a reassignment of surplus sugar under domestic cane sugar allotments of 325,000 short tons raw value (STRV) to imports, and increased the fiscal year (FY) 2011 raw sugar tariff-rate quota (TRQ) by...

  18. Probing the Geometry of the Inner Vestibule of BK Channels with Sugars

    Science.gov (United States)

    Brelidze, Tinatin I.; Magleby, Karl L.

    2005-01-01

    The geometry of the inner vestibule of BK channels was probed by examining the effects of different sugars in the intracellular solution on single-channel current amplitude (unitary current). Glycerol, glucose, and sucrose decreased unitary current through BK channels in a concentration- and size-dependent manner, in the order sucrose > glucose > glycerol, with outward currents being reduced more than inward currents. The fractional decrease of outward current was more directly related to the fractional hydrodynamic volume occupied by the sugars than to changes in osmolality. For concentrations of sugars ≤1 M, the i/V plots for outward currents in the presence and absence of sugar superimposed after scaling, and increasing K+ i from 150 mM to 2 M increased the magnitudes of the i/V plots with little effect on the shape of the scaled curves. These observations suggest that sugars ≤1 M reduce outward currents mainly by entering the inner vestibule and reducing the movement of K+ through the vestibule, rather than by limiting diffusion-controlled access of K+ to the vestibule. With 2 M sucrose, the movement of K+ into the inner vestibule became diffusion limited for 150 mM K+ i and voltages >+100 mV. Increasing K+ i then relieved the diffusion limitation. An estimate of the capture radius based on the 5 pA diffusion-limited current for channels without the ring of negative charge at the entrance to the inner vestibule was 2.2 Å. Adding the radius of a hydrated K+ (6–8 Å) then gave an effective radius for the entrance to the inner vestibule of 8–10 Å. Such a functionally wide entrance to the inner vestibule together with our observation that even small concentrations of sugar in the inner vestibule reduce unitary current suggest that a wide inner vestibule is required for the large conductance of BK channels. PMID:16043773

  19. Comparative anatomy of the peduncles of Thai Sugar Palms provides insight on putative sugar transport mechanisms

    DEFF Research Database (Denmark)

    Somjaiai, Pananun; Barfod, Anders; Jampeetong, Arunothai

    Inflorescences of sugar palms exude copious amounts of sugar-rich sap, when their peduncles are scarred. In Thailand this phenomenon form the basis of a widespread cottage industry based on species such as Arenga pinnata, Borassus flabellifera, Cocos nucifera and Nypa fruticans. The extracted sugar...... sap is used mainly for jaggery, syrup and different types of beverages. In this study we looked for anatomical correlates of the elevated sap flow in injured peduncles of sugar palms. Despite a limited sample size we observed that sugar producing palms differ from the reference palm Chamaedorea...

  20. Sugar in Infants, Children and Adolescents

    DEFF Research Database (Denmark)

    Mis, Nataša Fidler; Braegger, Christian; Bronsky, Jiri

    2017-01-01

    The consumption of sugars, particularly sugar-sweetened beverages (SSBs; beverages or drinks that contain added caloric sweeteners (i.e. sucrose, high-fructose corn syrup, fruit-juice concentrates), in European children and adolescents exceeds current recommendations. This is of concern because...... there is no nutritional requirement for free sugars, and infants have an innate preference for sweet taste, which may be modified and reinforced by pre- and postnatal exposures. Sugar containing beverages/free sugars increase the risk for overweight/obesity and dental caries, can result in poor nutrient supply...... and reduced dietary diversity and may be associated with increased risk of type 2 diabetes mellitus, cardiovascular risk, and other health effects. The term 'free sugars', includes all monosaccharides/disaccharides added to foods/beverages by the manufacturer/cook/consumer, plus sugars naturally present...

  1. Sugar in infants, children and adolescents

    DEFF Research Database (Denmark)

    Mis, Nataša Fidler; Braegger, Christian; Bronsky, Jiri

    2017-01-01

    The consumption of sugars, particularly sugar-sweetened beverages (SSBs; beverages or drinks that contain added caloric sweeteners (i.e. sucrose, high-fructose corn syrup, fruit-juice concentrates), in European children and adolescents exceeds current recommendations. This is of concern because...... there is no nutritional requirement for free sugars, and infants have an innate preference for sweet taste, which may be modified and reinforced by pre- and postnatal exposures. Sugar containing beverages/free sugars increase the risk for overweight/obesity and dental caries, can result in poor nutrient supply...... and reduced dietary diversity and may be associated with increased risk of type 2 diabetes mellitus, cardiovascular risk, and other health effects. The term 'free sugars', includes all monosaccharides/disaccharides added to foods/beverages by the manufacturer/cook/consumer, plus sugars naturally present...

  2. An Intergrated Sustainability Assessment of the Swedish Sugar Production System from a Life-Cycle Perspective: 2003-2015

    Directory of Open Access Journals (Sweden)

    Barry Ness

    2011-06-01

    Full Text Available The article presents a sustainability assessment of the beet-to-sugar production system in Sweden from 2003 until 2015. It focuses on the life-cycle phases of beet growing, beet transport and sugar processing. Based on the Swedish sustainable development strategy, eight indicators in environmental and socio-economic domains based significantly on EU price and production quota changes are assessed. The study also appraises the autumn wheat-to-flour production system as an alternative scenario to provide a better understanding of the overall impacts on the region of the effects of the EU price and quota changes. The method used is a system analysis (simulation model developed with the software STELLA 9.1. The study is a part of a broader regional sustainability assessment that focuses on the sugar sector in Sweden. Model results of the combined sugar and flour systems show general declines in agricultural landscape diversity and revenues earned in the region with only slight decreases in the number of full-time jobs in the region. Results also reveal decreases in the amount of nutrient runoff, fossil fuel energy use, greenhouse gas releases and field chemical use, with more substantial decreases in biodiversity via the suspension of organic beet growing in the region.

  3. Acid-producing capacity from sugars and sugar alcohols among Lactobacillus isolates collected in connection with radiation therapy.

    Science.gov (United States)

    Almståhl, Annica; Rudbäck, Helena; Basic, Amina; Carlén, Anette; Alstad, Torgny

    2017-12-01

    To investigate the acid-producing capacity from sugars and sugar alcohols of oral Lactobacillus collected in connection with radiation therapy (RT) to the head and neck region. Lactobacillus were collected from the tongue, buccal mucosa and supragingival plaque in 24 patients before, during, and after RT. The acid-producing capacity of Lactobacillus isolates (n=211) was analyzed using a colorimetric fermentation test in microtiter plates. Solutions containing 2% sugars (sucrose, glucose, fructose, lactose) or sugar-alcohols (sorbitol and xylitol) were used. After 24h of incubation, bacterial acid-producing capacity was determined as strong (pH6). Data regarding intake frequency of sugar-rich products and products with sugar-alcohols was collected. The highest acid-producing capacity using the sugars was seen for isolates collected during RT. Sorbitol was fermented to a higher extent during and post RT, especially among isolates from plaque. Lactobacillus fermenting xylitol showed the highest acid-producing capacity during RT (psugar-rich products or sugar-alcohol containing products and Lactobacillus acid-producing capacity, were found. The results suggest that Lactobacillus isolates, collected from the tongue, buccal mucosa and supragingival plaque, have a higher acid-producing capacity using sugars and sugar-alcohols during RT than one year post RT. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. The Effect of Sugar Price Policy on U.S. Imports of Processed Sugar-containing foods

    OpenAIRE

    Jabara, Cathy L.

    1988-01-01

    This paper examines the effects of sugar protection, as well as the effects of these other factors, on U.S. demand for imports of sugar-containing products. The paper also addresses two main issues: (1) whether substitution of alternative sweeteners has allowed U.S. food manufactures to reduce the competitive advantage provided to foreign manufactures by U.S. sugar policies, and (2) which countries -- developed or developing -- have been able to increase their exports of sugar-containing prod...

  5. KARAKTERISASI KIMIA, FISIKOKIMIA DAN ORGANOLEPTIK JAM DAN JELLY JONJOT LABU KUNING (Cucurbita maxima [Chemical, Physicochemical and Sensory Characterization of Jam and Jelly Made from Pumpkin Strands (Cucurbita maxima

    Directory of Open Access Journals (Sweden)

    Murdijati Gardjito1

    2006-08-01

    Full Text Available The objective of this research was to examine the contents of pectin,carotene and sugars of the pumpkin strands, and to evaluate the effects of sugar addition and pH on the chemical, physical, and sensory characteristics of jam and jelly made from pumpkin strands. The preparation of jam and jelly were conducted with sugar addition of 55%, 60%, and 65% and pH values of 3.0, 3.2, and 3.4. The products were analyzed for moisture, sugar,carotene, and soluble solid, and were evaluated for firmness and sensory properties.The results showed that the moisture content of jelly was between 26.36 - 35.27% and that of the jam between 39.53 - 45.67%. Beta-carotene of jelly was between 241.79 - 404.42 RE/100g (db and that of jam was between 235.58 - 487.51 RE/100g (db. The decreasing of pH and increasing of sugar addition tended to increase the content of reducing sugars and soluble solid of the products. Jelly could be made from pumpkin strands by addition of 55% or 60% sugar, and the firmness increased by decreasing of pH value. There were no significance differences between the treatments for color, flavor and overall preference score. The addition of 60% sugar and pH of 3.2 was the best condition for making jam and jelly from pumpkin strands.

  6. Minimization of sucrose losses in sugar industry by pH and temperature optimization

    International Nuclear Information System (INIS)

    Panpae, Kornvalai; Jaturonrusmee, Wasna; Mingvanish, Withawat; Santudrob, Kittisak; Triphanpitak, Siriphan

    2008-01-01

    Invert sugar has several disadvantage properties that play an important role in many food applications. It has a high affinity for water and is the cause of making products retain moisture. Invert sugar also affects the carmelization process, producing a browning effect. In this study, the possibility of minimization of sucrose inversion during the industrial production of sugar cane was investigated by the variation of the important parameters, i.e. temperature and pH of sugar cane juice for each of samples. The amounts of sucrose and reducing sugar alerting during the sucrose inversion process were determined by the values of % Pol and % reducing sugar (% RS), respectively. Starting with the study of temperature and pH effects of the sucrose solution with the concentration of 16 Brix, used as a sample model, it was found that no change in amounts of reducing sugar and sucrose was observed at room temperature (34 degree Celsius) in the pH range of 5-11. At pH 3, the amounts of reducing sugar increased and the amount of sucrose decreased as the time increased. These indicated that the process of sucrose inversion should better occur in more acidic solutions. Compared to the room temperature, it was found that the increment of temperature led to enhance the process of sucrose inversion. This was depicted by higher values of % RS and lower value of % Pol as the temperatures were elevated. The experiments were also done with real sugar cane juice, i.e. first, last, and mixed juice. The tendency of changes of the amounts of reducing sugar and sucrose in sugar cane samples by varying temperature and pH were found to resemble to those for the sample model. The increment of temperatures have also affected on a reduction of amounts of sucrose in each sugar cane juice. In addition, it could be concluded that the acidity of the solution affects sucrose easier to be broken down to glucose and fructose molecules. (author)

  7. Sugar-free medicines are counterproductive.

    Science.gov (United States)

    Sundar, S

    2012-09-01

    Sugar in food and drinks is responsible for the poor dental health of many children and adults. On the other hand, there is no evidence that the small amount of sugar in medicines has been responsible for any dental problems. A recent British Heart Foundation survey found that nearly one in three UK children are eating sweets, chocolate and crisps three or more times a day. Hence it is futile administering sugar-free medicine to a child consuming lot of sweets. Moreover, sugar in medicines makes them palatable and bitter medicines inevitably affect compliance with the prescribed treatment. Poor compliance leads to inadequate treatment of illness and consequently increases the risk of complications from illness. Hence sugar-free medicines promoted as a public health policy could have actually caused more harm than any meaningful net benefit. There is an urgent need for a healthy debate and a fresh look at the policy of promoting sugar-free medicines.

  8. Three new hydrochlorothiazide cocrystals: Structural analyses and solubility studies

    Science.gov (United States)

    Ranjan, Subham; Devarapalli, Ramesh; Kundu, Sudeshna; Vangala, Venu R.; Ghosh, Animesh; Reddy, C. Malla

    2017-04-01

    Hydrochlorothiazide (HCT) is a diuretic BCS class IV drug with poor aqueous solubility and low permeability leading to poor oral absorption. The present work explores the cocrystallization technique to enhance the aqueous solubility of HCT. Three new cocrystals of HCT with water soluble coformers phenazine (PHEN), 4-dimethylaminopyridine (DMAP) and picolinamide (PICA) were prepared successfully by solution crystallization method and characterized by single crystal X-ray diffraction (SCXRD), powder X-ray diffraction (PXRD), fourier transform -infraredspectroscopy (FT-IR), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). Structural characterization revealed that the cocrystals with PHEN, DMAP and PICA exists in P21/n, P21/c and P21/n space groups, respectively. The improved solubility of HCT-DMAP (4 fold) and HCT-PHEN (1.4 fold) cocrystals whereas decreased solubility of HCT-PICA (0.5 fold) as compared to the free drug were determined after 4 h in phosphate buffer, pH 7.4, at 25 °C by using shaking flask method. HCT-DMAP showed a significant increase in solubility than all previously reported cocrystals of HCT suggest the role of a coformer. The study demonstrates that the selection of coformer could have pronounced impact on the physicochemical properties of HCT and cocrystallization can be a promising approach to improve aqueous solubility of drugs.

  9. Effect of Germination and Fermentation on Carbohydrate Composition of Australian Sweet Lupin and Soybean Seeds and Flours.

    Science.gov (United States)

    Kaczmarska, Kornelia T; Chandra-Hioe, Maria V; Zabaras, Dimitrios; Frank, Damian; Arcot, Jayashree

    2017-11-22

    This study investigated the effect of germination and fermentation on the composition of carbohydrates in Australian sweet lupin. Specifically, the amount of sugars (sucrose, fructose, and glucose), starch, oligosaccharides (verbascose, stachyose, and raffinose), and dietary fiber were measured in germinated lupin seeds and fermented lupin flour, and compared with those in soy. High performance liquid chromatography coupled with refractive index was employed for quantitation of sugars, starch, and oligosaccharides, and gas chromatography coupled with a flame ionization detector was used for quantitation of simple sugars in total, and soluble, and insoluble dietary fiber. The enzyme activities of α-amylase and α-glucosidase were compared before and after germination or fermentation. The α-amylase activity in germinated lupin increased to  ∼17 nmol/mL/min/0.1 g and in germinated soy∼32; in fermented lupin, the activity increased to ∼52, while in fermented soy it decreased to ∼20. In general, germination or fermentation decreased the oligosaccharide content, and increased the total sugar in samples (p Fermentation with yogurt culture lowered the content of total oligosaccharides due to 94% decrease in stachyose. Total oligosaccharides in soy flour prior to fermentation were 180 mg/g and significantly decreased to ∼124 mg/g in fermented soy. Germination did not affect the starch content. There was no significant change in the amounts of total, soluble, and insoluble dietary fiber after germination or fermentation of lupin except for galactose, which was significantly reduced in germinated lupin seeds. Soluble dietary fiber in germinated soy significantly increased. Germination and fermentation are simple and effective techniques to reduce the oligosaccharides while maintaining the composition of dietary fibers.

  10. MMP-15 is upregulated in preeclampsia, but does not cleave endoglin to produce soluble endoglin.

    Directory of Open Access Journals (Sweden)

    Tu'uhevaha J Kaitu'u-Lino

    Full Text Available Preeclampsia is a major pregnancy complication, characterized by severe endothelial dysfunction, hypertension and maternal end-organ damage. Soluble endoglin is an anti-angiogenic protein released from placenta and thought to play a central role in causing the endothelial dysfunction and maternal organ injury seen in severe preeclampsia. We recently reported MMP-14 was the protease producing placentally-derived soluble endoglin by cleaving full-length endoglin present on the syncytiotrophoblast surface. This find identifies a specific drug target for severe preeclampsia; interfering with MMP-14 mediated cleavage of endoglin could decrease soluble endoglin production, ameliorating clinical disease. However, experimental MMP-14 inhibition alone only partially repressed soluble endoglin production, implying other proteases might have a role in producing soluble endoglin. Here we investigated whether MMP-15--phylogenetically the closest MMP relative to MMP-14 with 66% sequence similarity--also cleaves endoglin to produce soluble endoglin. MMP-15 was localized to the syncytiotrophoblast layer of the placenta, the same site where endoglin was localized. Interestingly, it was significantly (p = 0.03 up-regulated in placentas from severe early-onset preeclamptic pregnancies (n = 8 compared to gestationally matched preterm controls (n = 8. However, siRNA knockdown of MMP-15 yielded no significant decrease of soluble endoglin production from either HUVECs or syncytialised BeWo cells in vitro. Importantly, concurrent siRNA knockdown of both MMP-14 and MMP-15 in HUVECS did not yield further decrease in soluble endoglin production compared to MMP-14 siRNA alone. We conclude MMP-15 is up-regulated in preeclampsia, but does not cleave endoglin to produce soluble endoglin.

  11. Respiration-Dependent Utilization of Sugars in Yeasts: a Determinant Role for Sugar Transporters

    OpenAIRE

    Goffrini, Paola; Ferrero, Iliana; Donnini, Claudia

    2002-01-01

    In many yeast species, including Kluyveromyces lactis, growth on certain sugars (such as galactose, raffinose, and maltose) occurs only under respiratory conditions. If respiration is blocked by inhibitors, mutation, or anaerobiosis, growth does not take place. This apparent dependence on respiration for the utilization of certain sugars has often been suspected to be associated with the mechanism of the sugar uptake step. We hypothesized that in many yeast species, the permease activities fo...

  12. Plutonium solubilities

    International Nuclear Information System (INIS)

    Puigdomnech, I.; Bruno, J.

    1991-02-01

    Thermochemical data has been selected for plutonium oxide, hydroxide, carbonate and phosphate equilibria. Equilibrium constants have been evaluated in the temperature range 0 to 300 degrees C at a pressure of 1 bar to T≤100 degrees C and at the steam saturated pressure at higher temperatures. Measured solubilities of plutonium that are reported in the literature for laboratory experiments have been collected. Solubility data on oxides, hydroxides, carbonates and phosphates have been selected. No solubility data were found at temperatures higher than 60 degrees C. The literature solubility data have been compared with plutonium solubilities calculated with the EQ3/6 geochemical modelling programs, using the selected thermodynamic data for plutonium. (authors)

  13. Sugar uptake, carbohydrate metabolism and ANA and RNA contents of paecilomyces violacea mats arising from gamma irradiated inocula

    International Nuclear Information System (INIS)

    El-Zawahry, A.Y.; Salama, A.M.; Nadia, M.M.; Abo-El-Khair, I.A.

    1988-01-01

    Irradiated inocula of paecilomyces violacea with the lowest doses 0.1 and 0.25 KGy increased the amounts of sugar uptake over control value by 11.43% and 19.17% respectively. promoted sugar uptake at such doses was coupled by a consequent drop in the monosaccharide content of the medium amounting to 30.62% and 58.53% and 58.53% below control value respectively. Higher doses of gamma radiation were associated with decreased rates of sugar uptake by the respective mats. Polysaccharide synthesis was inhibited gradually with increasing the irradiation dose. Irradiated incocula of P. Violacea produced mats which showed a general inconsistent decrease in RNA content and a general limited increase in the DNA content with the increase of irradiation dose

  14. Sugar and Sugar Derivatives in Residues Produced from the UV Irradiation of Astrophysical Ice Analogs

    Science.gov (United States)

    Nuevo, M.; Sandford, S. A.; Cooper, G.

    2016-01-01

    A large variety and number of organic compounds of prebiotic interest are known to be present in carbonaceous chondrites. Among them, one sugar (dihydroxyacetone) as well as several sugar acids, sugar alcohols, and other sugar derivatives have been reported in the Murchison and Murray meteorites. Their presence, along with amino acids, amphiphiles, and nucleobases strongly suggests that molecules essential to life can form abiotically under astrophysical conditions. This hypothesis is supported by laboratory studies on the formation of complex organic molecules from the ultraviolet (UV) irradiation of simulated astrophysical ice mixtures consisting of H2O, CO, CO2, CH3OH, CH4, NH3, etc., at low temperature. In the past 15 years, these studies have shown that the organic residues recovered at room temperature contain amino acids, amphiphiles, nucleobases, as well as other complex organics. However, no systematic search for the presence of sugars and sugar derivatives in laboratory residues have been reported to date, despite the fact that those compounds are of primary prebiotic significance. Indeed, only small (up to 3 carbon atoms) sugar derivatives including glycerol and glyceric acid have been detected in residues so far.

  15. Co-ensiling as a new technique for long-term storage of agro-industrial waste with low sugar content prior to anaerobic digestion.

    Science.gov (United States)

    Hillion, Marie-Lou; Moscoviz, Roman; Trably, Eric; Leblanc, Yoann; Bernet, Nicolas; Torrijos, Michel; Escudié, Renaud

    2018-01-01

    Biodegradable wastes produced seasonally need an upstream storage, because of the requirement for a constant feeding of anaerobic digesters. In the present article, the potential of co-ensiling biodegradable agro-industrial waste (sugar beet leaves) and lignocellulosic agricultural residue (wheat straw) to obtain a mixture with low soluble sugar content was evaluated for long-term storage prior to anaerobic digestion. The aim is to store agro-industrial waste while pretreating lignocellulosic biomass. The dynamics of co-ensiling was evaluated in vacuum-packed bags at lab-scale during 180 days. Characterization of the reaction by-products and microbial communities showed a succession of metabolic pathways. Even though the low initial sugars content was not sufficient to lower the pH under 4.5 and avoid undesirable fermentations, the methane potential was not substantially impacted all along the experiment. No lignocellulosic damages were observed during the silage process. Overall, it was shown that co-ensiling was effective to store highly fermentable fresh waste evenly with low sugar content and offers new promising possibilities for constant long-term supply of industrial anaerobic digesters. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. A free sugars daily value (DV) identifies more "less healthy" prepackaged foods and beverages than a total sugars DV.

    Science.gov (United States)

    Bernstein, Jodi T; Labonté, Marie-Ève; Franco-Arellano, Beatriz; Schermel, Alyssa; L'Abbé, Mary R

    2018-04-01

    Regulatory changes in Canada will require food labels to have a benchmark [% Daily Value, %DV] for total sugars, based on 100 g/day, while US labels will require a %DV for added sugars, based on 50 g/day. The objective of this study was to compare two labelling policies, a total sugars DV (100 g/day) and a free sugars DV (50 g/day) on food labels. This cross-sectional analysis of the Food Label Information Program database focussed on top sources of total sugars intake in Canada (n = 6924 foods). Products were categorized as "less healthy" using two sets of criteria: a) free sugars levels exceeding the WHO guidelines (≥10% energy from free sugars); and b) exceeding healthfulness cut-offs of the Food Standards Australia New Zealand Nutrient Profiling Scoring Criterion (FSANZ-NPSC). The proportion of "less healthy" products with ≥15%DV (defined as "a lot" of sugars i.e. high in sugars, based on Health Canada's %DV labelling footnote and educational message for dietary guidance) were compared for each sugar labelling scenario. The free sugars DV showed better alignment with both methods for assessing "healthfulness" than the total sugars DV. The free sugars DV identified a greater proportion of "less healthy" foods with ≥15%DV, based on both the FSANZ-NPSC (70% vs. 45%, p chocolate bars, confectionery, and frozen desserts categories. Compared to total sugars DV labelling, using a free sugars DV identified more "less healthy" foods. Findings support the adoption of free sugars labelling. Copyright © 2018 Elsevier Inc. All rights reserved.

  17. Aromatics extraction from pyrolytic sugars using ionic liquid to enhance sugar fermentability

    NARCIS (Netherlands)

    Li, X.; Luque-Moreno, L.C.; Oudenhoven, Stijn; Rehmann, L.; Kersten, Sascha R.A.; Schuur, Boelo

    2016-01-01

    Fermentative bioethanol production from pyrolytic sugars was improved via aromatics removal by liquid–liquid extraction. As solvents, the ionic liquid (IL) trihexyltetradecylphosphonium dicyanamide (P666,14[N(CN)2]) and ethyl acetate (EA) were compared. Two pyrolytic sugar solutions were created

  18. Biochemical and anatomical changes and yield reduction in rice (Oryza sativa L.) under varied salinity regimes.

    Science.gov (United States)

    Hakim, M A; Juraimi, Abdul Shukor; Hanafi, M M; Ismail, Mohd Razi; Selamat, Ahmad; Rafii, M Y; Latif, M A

    2014-01-01

    Five Malaysian rice (Oryza sativa L.) varieties, MR33, MR52, MR211, MR219, and MR232, were tested in pot culture under different salinity regimes for biochemical response, physiological activity, and grain yield. Three different levels of salt stresses, namely, 4, 8, and 12 dS m(-1), were used in a randomized complete block design with four replications under glass house conditions. The results revealed that the chlorophyll content, proline, sugar content, soluble protein, free amino acid, and yield per plant of all the genotypes were influenced by different salinity levels. The chlorophyll content was observed to decrease with salinity level but the proline increased with salinity levels in all varieties. Reducing sugar and total sugar increased up to 8 dS m(-1) and decreased up to 12 dS m(-1). Nonreducing sugar decreased with increasing the salinity levels in all varieties. Soluble protein and free amino acid also decreased with increasing salinity levels. Cortical cells of MR211 and MR232 did not show cell collapse up to 8 dS m(-1) salinity levels compared to susceptible checks (IR20 and BRRI dhan29). Therefore, considering all parameters, MR211 and MR232 showed better salinity tolerance among the tested varieties. Both cluster and principal component analyses depict the similar results.

  19. Biochemical and Anatomical Changes and Yield Reduction in Rice (Oryza sativa L. under Varied Salinity Regimes

    Directory of Open Access Journals (Sweden)

    M. A. Hakim

    2014-01-01

    Full Text Available Five Malaysian rice (Oryza sativa L. varieties, MR33, MR52, MR211, MR219, and MR232, were tested in pot culture under different salinity regimes for biochemical response, physiological activity, and grain yield. Three different levels of salt stresses, namely, 4, 8, and 12 dS m−1, were used in a randomized complete block design with four replications under glass house conditions. The results revealed that the chlorophyll content, proline, sugar content, soluble protein, free amino acid, and yield per plant of all the genotypes were influenced by different salinity levels. The chlorophyll content was observed to decrease with salinity level but the proline increased with salinity levels in all varieties. Reducing sugar and total sugar increased up to 8 dS m−1 and decreased up to 12 dS m−1. Nonreducing sugar decreased with increasing the salinity levels in all varieties. Soluble protein and free amino acid also decreased with increasing salinity levels. Cortical cells of MR211 and MR232 did not show cell collapse up to 8 dS m−1 salinity levels compared to susceptible checks (IR20 and BRRI dhan29. Therefore, considering all parameters, MR211 and MR232 showed better salinity tolerance among the tested varieties. Both cluster and principal component analyses depict the similar results.

  20. Microbiological Spoilage of High-Sugar Products

    Science.gov (United States)

    Thompson, Sterling

    The high-sugar products discussed in this chapter are referred to as chocolate, sugar confectionery (non-chocolate), liquid sugars, sugar syrups, and honey. Products grouped in the sugar confectionery category include hard candy, soft/gummy candy, caramel, toffee, licorice, marzipan, creams, jellies, and nougats. A common intrinsic parameter associated with high-sugar products is their low water activity (a w), which is known to inhibit the growth of most spoilage and pathogenic bacteria. However, spoilage can occur as a result of the growth of osmophilic yeasts and xerophilic molds (Von Richter, 1912; Anand & Brown, 1968; Brown, 1976). The a w range for high-sugar products is between 0.20 and 0.80 (Banwart, 1979; Richardson, 1987; Lenovich & Konkel, 1992; ICMSF, 1998; Jay, Loessner, & Golden, 2005). Spoilage of products, such as chocolate-covered cherries, results from the presence of yeasts in the liquid sugar brine or the cherry. Generally, the spoiled product will develop leakers. The chocolate covering the cherry would not likely be a source of yeast contamination.

  1. The Effect of Alkaline Concentration on Coconut Husk Crystallinity and the Yield of Sugars Released

    Science.gov (United States)

    Sangian, H. F.; Widjaja, A.

    2018-02-01

    This work was to analyze the effect of alkaline concentration on coconut coir husk crystallinity and sugar liberated enzymatically. The data showed that the employing of alkaline on lignocellulose transformed the crystallinity. The XRD peaks increased highly which indicated that cellulose was more opened and exposed. After pretreatment, the chemical compositions (cellulose, hemicellulose, and lignin) were changed significantly. The employing 1% alkaline, the cellulosic content inclined if compared to that of non-pretreatment. When the alkaline concentration was added to 4%, the cellulose was decreased slightly which indicated that a part of cellulose and hemicellulose was dissolved into solution. It was found the alkaline pretreatment influenced by the biochemical reaction of treated substrates in producing the reducing sugars. The amounts of sugar liberated enzymatically of coconut husk treated by 1% and 4% alkaline increased to 0.26, and 0.24 g sugar/g (cellulose+hemicellulose), respectively, compared to that of native solid recorded at 0.18 g sugar/g (cellulose+hemicellulose).

  2. I Mend It With Sugar

    OpenAIRE

    Lindvall, Charlotta

    2015-01-01

    I mend it with sugar         Abstract   "Gluttony" and "sloth" is the sugar addictions best friend, or could it be that the addiction comes out of a disturbed hormone production caused by the environment that surrounds us? Trying to understand my own sugar addiction I weave in my personal story into my artistic research around this subject. The sugar might be the cause of the pandemic obesity and that's why it has to bee brought up into the light from its darkness down the basement of the fo...

  3. CHARACTERISTICS AND FERTILITY OF SUMATRAN TIGER SPERMATOZOA CRYOPRESERVED WITH DIFFERENT SUGARS.

    Science.gov (United States)

    Wayan Kurniani Karja, Ni; Fahrudin, Mokhamad; Setiadi, Mohamad Agus; Tumbelaka, Ligaya Ita; Sudarwati, Retno; Hastuti, Yohana Tri; Mulia, Bongot Huas; Widianti, Ardyta; Sultan, Keni; Terazono, Tsukasa; Namula, Zhao; Taniguchi, Masayasu; Tanihara, Fuminori; Takemoto, Tatsuya; Kikuchi, Kazuhiro; Sato, Yoko; Otoi, Takeshige

    Cryopreservation of semen is one of the most important methods for the preservation of endangered tigers. This study evaluated the effects of sugar supplementation on the cryosurvival of spermatozoa from Sumatran tigers (Panthera tigris sumatrae). The post-thaw characteristics and fertility of spermatozoa cryopreserved with different sugars (glucose, lactose, and trehalose) were evaluated using heterologous in-vitro fertilisation with cat oocytes. All parameters of post-thaw spermatozoa significantly decreased as compared with those of fresh spermatozoa. The index of sperm motility for semen cryopreserved with lactose was significantly higher than that for semen cryopreserved with trehalose. The percentage of total fertilisation for tiger spermatozoa cryopreserved with trehalose was significantly lower than that for control cat spermatozoa. Our findings indicated that supplementation with lactose or glycerol as the main sugar in the egg yolk extender resulted in a better motility and fertility potential for post-thawed spermatozoa.

  4. 75 FR 23631 - Sugar Re-Export Program, the Sugar-Containing Products Re-Export Program, and the Polyhydric...

    Science.gov (United States)

    2010-05-04

    ... Part 1530 Sugar Re-Export Program, the Sugar-Containing Products Re-Export Program, and the Polyhydric...), Additional U.S. Note 6, which authorizes entry of raw cane sugar under subheading 1701.11.20 of the HTS for the production of polyhydric alcohols, except polyhydric alcohols for use as a substitute for sugar in...

  5. Low blood sugar - newborns

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/007306.htm Low blood sugar - newborns To use the sharing features on this page, please enable JavaScript. A low blood sugar level in newborn babies is also ...

  6. Decolorization of turbid sugar juice from sugar factory using waste powdered carbon

    Science.gov (United States)

    Aljohani, Hind; Ahmed, Youssef; El-Shafey, Ola; El-Shafey, Shaymaa; Fouad, Rasha; Shoueir, Kamel

    2018-03-01

    Waste management of powdered activated carbon from cyclone of some sugar factories was used for decolorization of sugar mud juice (SMJ) in this study. The presence of powdered activated carbon waste (PACW) was admitted again for their use in SMJ decolorization. The determined specific surface area are typically S BET = 613.887 m2/g and the pore distribution lies in mesoporous domain. Color removal (CR%) and decolorization capacity (DC) of the characterized PACW are similar to those of decolorants used at this time for sugar refining. The CR% with PACW reached 81.03% at pH7.0 and dosed in the amount 0.5 g/50 ml of SMJ. There are two acceptable mechanisms illustrates the attachments between phenols and carboxylate ions. In this paper, we put a simple and rapid dark liquid decolorization by controlling rejected carbon waste, which will be useful for treatment of dark liquid sugar.

  7. Added sugars in kids' meals from chain restaurants.

    Science.gov (United States)

    Scourboutakos, Mary J; Semnani-Azad, Zhila; L'Abbé, Mary R

    2016-06-01

    To analyze the added sugars in kids' meals from Canadian chain restaurants in relation to the World Health Organization's proposed sugar recommendation (less than 5% of total daily calories should come from added sugars) and current recommendation (less than 10% of total daily calories should come from added sugars). Total sugar levels were retrieved from the websites of 10 fast-food and 7 sit-down restaurants in 2010. The added sugar levels in 3178 kids' meals from Canadian chain restaurants were calculated in 2014 (in Toronto, Canada) by subtracting all naturally occurring sugars from the total sugar level. The average amount of added sugars in restaurant kids' meals (25 ± 0.36 g) exceeded the WHO's proposed daily recommendation for sugar intake. There was a wide range of added sugar levels in kids' meals ranging from 0 g to 114 g. 50% of meals exceeded the WHO's proposed daily sugar recommendation, and 19% exceeded the WHO's current daily sugar recommendation. There is a wide range of sugar levels in kids' meals from restaurants, and many contain more than a day's worth of sugar.

  8. Sugar addiction: the state of the science.

    Science.gov (United States)

    Westwater, Margaret L; Fletcher, Paul C; Ziauddeen, Hisham

    2016-11-01

    As obesity rates continue to climb, the notion that overconsumption reflects an underlying 'food addiction' (FA) has become increasingly influential. An increasingly popular theory is that sugar acts as an addictive agent, eliciting neurobiological changes similar to those seen in drug addiction. In this paper, we review the evidence in support of sugar addiction. We reviewed the literature on food and sugar addiction and considered the evidence suggesting the addictiveness of highly processed foods, particularly those with high sugar content. We then examined the addictive potential of sugar by contrasting evidence from the animal and human neuroscience literature on drug and sugar addiction. We find little evidence to support sugar addiction in humans, and findings from the animal literature suggest that addiction-like behaviours, such as bingeing, occur only in the context of intermittent access to sugar. These behaviours likely arise from intermittent access to sweet tasting or highly palatable foods, not the neurochemical effects of sugar. Given the lack of evidence supporting it, we argue against a premature incorporation of sugar addiction into the scientific literature and public policy recommendations.

  9. Decreasing the Burden of Type 2 Diabetes in South Africa: The Impact of Taxing Sugar-Sweetened Beverages

    OpenAIRE

    Manyema, Mercy; Veerman, J. Lennert; Chola, Lumbwe; Tugendhaft, Aviva; Labadarios, Demetre; Hofman, Karen

    2015-01-01

    Introduction Type 2 diabetes poses an increasing public health burden in South Africa (SA) with obesity as the main driver of the epidemic. Consumption of sugar sweetened beverages (SSBs) is linked to weight gain and reducing SSB consumption may significantly impact the prevalence of obesity and related diseases. We estimated the effect of a 20% SSB tax on the burden of diabetes in SA. Methods and Findings We constructed a life table-based model in Microsoft Excel (2010). Consumption data fro...

  10. A Loose Relationship: Incomplete H+/Sugar Coupling in the MFS Sugar Transporter GlcP.

    Science.gov (United States)

    Bazzone, Andre; Zabadne, Annas J; Salisowski, Anastasia; Madej, M Gregor; Fendler, Klaus

    2017-12-19

    The glucose transporter from Staphylococcus epidermidis, GlcP Se , is a homolog of the human GLUT sugar transporters of the major facilitator superfamily. Together with the xylose transporter from Escherichia coli, XylE Ec , the other prominent prokaryotic GLUT homolog, GlcP Se , is equipped with a conserved proton-binding site arguing for an electrogenic transport mode. However, the electrophysiological analysis of GlcP Se presented here reveals important differences between the two GLUT homologs. GlcP Se , unlike XylE Ec , does not perform steady-state electrogenic transport at symmetrical pH conditions. Furthermore, when a pH gradient is applied, partially uncoupled transport modes can be generated. In contrast to other bacterial sugar transporters analyzed so far, in GlcP Se sugar binding, translocation and release are also accomplished by the deprotonated transporter. Based on these experimental results, we conclude that coupling of sugar and H + transport is incomplete in GlcP Se . To verify the viability of the observed partially coupled GlcP Se transport modes, we propose a universal eight-state kinetic model in which any degree of coupling is realized and H + /sugar symport represents only a specific instance. Furthermore, using sequence comparison with strictly coupled XylE Ec and similar sugar transporters, we identify an additional charged residue that may be essential for effective H + /sugar symport. Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  11. The effect of alpha amylase enzyme on quality of sweet sorghum juice for chrystal sugar

    Science.gov (United States)

    Marwati, T.; Cahyaningrum, N.; Widodo, S.; Astiati, U. T.; Budiyanto, A.; Wahyudiono; Arif, A. B.; Richana, N.

    2018-01-01

    Sweet sorghum juice (Sorghum bicolor L. Moench) has characteristics similar to sugar cane juice and potentially used for sugar substitutes that can support food security. Nevertheless the sweet sorghum juicecontain starch which impede sorghum sugar crystallization. Therefore, research on the enzymatic process is needed to convert starch into reducing sugar. The experimental design used was the Factorial Randomized Design with the first factor was alpha amylase enzyme concentration (0, 20, 40, 60, 80, 100, 120 μL/100 mL) and second factor was incubation time (0, 30, 60, 90 minute) at temperature 100°C. The experiment was conducted on fresh sweet sorghum. The results showed that the addition of the alpha amylase enzyme increased the content of reducing sugar and decreased levels of starch. Elevating concentration of alpha amylase enzyme will increase the reducing sugar content in sweet sorghum juice. The optimum alpha amylase enzyme concentration to produce the highest total sugar was 80 μL/100 mL of sweet sorghum juice with the optimum incubation time was 90 minutes. The results of this study are expected to create a new sweetener for sugar substitution. From the economic prospective aspect, sorghum is a potential crop and can be relied upon to support the success of the food diversification program which further leads to the world food security

  12. Determination of mangiferin solubility in solvents used in the biopharmaceutical industry

    Directory of Open Access Journals (Sweden)

    Jhoany Acosta

    2016-04-01

    Full Text Available Context: Pharmacological properties and studies of methods of extraction of mangiferin have been reported, but there are not studies related to the solubility of mangiferin in the solvents used in the pharmaceutical industry. Aims: Study the solubility of mangiferin in different solvents used in the pharmaceutical industry. Methods: The mangiferin used had a purity of 97.3% determined by High-Performance Liquid Chromatographic (HPLC, and solubility measurements were made in ethanol, methanol, water, acetone, diethyl ether, and hexane at 5, 15, 30, 40, 50 and 600C of temperature. The mangiferin concentrations were determined by ultraviolet spectrometry at 254 nm. The experimental solubility data were correlated with the Van´t Hoff equation and the dissolution heat determined. Results: The solubility of mangiferin in pure solvents decreases with increasing of temperature and in the following order: ethanol>methanol>water>diethyl ether>acetone>n hexane. Conclusions: This results indicated that mangiferin is slightly soluble in ethanol, sparingly soluble in methanol and water and practically insoluble in diethyl ether, acetone, and n-hexane.

  13. Effect of infection by chlorotic spot virus on 14CO2 fixation in leaves of groundnut Arachis hypogea L

    International Nuclear Information System (INIS)

    Sreenivasulu, P.; Nayudu, M.V.

    1980-01-01

    Photosynthetic incorporation of 14 CO 2 into leaves of groundnut infected by chlorotic spot virus (GCSV) was slightly more at stages 2 and 5 less at stage 4 as compared to control. 14 C incorporation into the alcohol soluble fraction of infected leaves followed the same trend as total 14 CO 2 fixation but in the alcohol-insoluble fraction the same was less at all the sampled stages. 14 C in the alcohol-soluble fraction of fed leaves of both types (stage 5) decreased with time along with simultaneous increase in alcohol-insoluble fraction. The proportion of 14 C incorporated into organic acids, amino acids and sugars was same in both the samples at stage 2, greater into organic and amino acids and less into sugars at stages 4 and 5, and at 12 and 24 hr time periods of stage 5 of virus infected leaves when compared to healthy ones. 14 C incorporated into total sugars and organic acids of infected leaves followed that of total 14 C fixation, and varied in individual sugars and organic acids. 14 C in sugars of both type of leaves decreased with time and with simultaneous increase in organic and amino acids. 14 C incorporated into virus infected leaf proteins was more when compared to healthy leaves. (auth.)

  14. Solubility of calcium in CaO-CaCl2

    International Nuclear Information System (INIS)

    Perry, G.S.; Shaw, S.J.

    1991-06-01

    The Direct Oxide Reduction (DOR) process is well established as a process to produce plutonium metal from plutonium dioxide by reaction with calcium. Calcium chloride is added to dissolve the calcium oxide produced, allowing the metal to coalesce into a button. Since calcium metal melts at 840 0 C and DOR can take place successfully below this temperature, it is likely calcium dissolved in calcium chloride reacts with the plutonium dioxide. The solubility of calcium in calcium chloride is reasonably well established but the effect of the CaO formed during the DOR process on the solubility of calcium has not been previously determined. For this reason the solubility of calcium in CaCl 2 -CaO melts at 800 o C has been studied. The solubility decreases from 2.7 mol % in CaCl 2 to 0.4 mol % in 9 mol % CaO-CaCl 2 . (author)

  15. Sugar palm (Argena pinnata). Potential of sugar palm for bio-ethanol production

    NARCIS (Netherlands)

    Elbersen, H.W.; Oyen, L.P.A.

    2010-01-01

    The energetic and economic feasibility of bioethanol production from sugar palm is virtually unknown. A positive factor are the potentially very high yields while the long non-productive juvenile phase and the high labor needs can be seen as problematic. Expansion to large scale sugar palm

  16. A rapid method for simultaneous quantification of 13 sugars and sugar alcohols in food products by UPLC-ELSD.

    Science.gov (United States)

    Koh, Dong-Wan; Park, Jae-Woong; Lim, Jung-Hoon; Yea, Myeong-Jai; Bang, Dae-Young

    2018-02-01

    A novel, rapid, simultaneous analysis method for five sugars (fructose, glucose, sucrose, maltose, and lactose) and eight sugar alcohols (erythritol, xylitol, sorbitol, mannitol, inositol, maltitol, lactitol, and isomalt) was developed using UPLC-ELSD, without derivatization. The analysis conditions, including the gradient conditions, modifier concentration and column length, were optimized. Thirteen sugars and sugar alcohols were separated well and the resolution of their peaks was above 1.0. Their optimum analysis condition can be analyzed within 15min. Standard curves for sugars and sugar alcohols with concentrations of 5.0-0.1% and 2.0-0.05% are presented herein, and their correlation coefficients are found to be above 0.999 and the limit of detection (LOD) was around 0.006-0.018%. This novel analysis system can be used for foodstuffs such as candy, chewing gum, jelly, chocolate, processed chocolate products, and snacks containing 0.21-46.41% of sugars and sugar alcohols. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Yield of glyphosate-resistant sugar beets and efficiency of weed management systems with glyphosate and conventional herbicides under German and Polish crop production.

    Science.gov (United States)

    Nichterlein, Henrike; Matzk, Anja; Kordas, Leszek; Kraus, Josef; Stibbe, Carsten

    2013-08-01

    In sugar beet production, weed control is one of the most important and most expensive practices to ensure yield. Since glyphosate-resistant sugar beets are not yet approved for cultivation in the EU, little commercial experience exists with these sugar beets in Europe. Experimental field trials were conducted at five environments (Germany, Poland, 2010, 2011) to compare the effects of glyphosate with the effects of conventional weed control programs on the development of weeds, weed control efficiency and yield. The results show that the glyphosate weed control programs compared to the conventional methods decreased not only the number of herbicide applications but equally in magnitude decreased the dosage of active ingredients. The results also showed effective weed control with glyphosate when the weed covering was greater and sugar beets had a later growth stage of four true leaves. Glyphosate-resistant sugar beets applied with the glyphosate herbicide two or three times had an increase in white sugar yield from 4 to 18 % in comparison to the high dosage conventional herbicide systems. In summary, under glyphosate management sugar beets can positively contribute to the increasingly demanding requirements regarding efficient sugar beet cultivation and to the demands by society and politics to reduce the use of chemical plant protection products in the environment.

  18. Biogas from sugar beet press pulp as substitute of fossil fuel in sugar beet factories.

    Science.gov (United States)

    Brooks, L; Parravicini, V; Svardal, K; Kroiss, H; Prendl, L

    2008-01-01

    Sugar beet press pulp (SBP) accumulates as a by-product in sugar factories and it is generally silaged or dried to be used as animal food. Rising energy prices and the opening of the European Union sugar market has put pressure on the manufacturers to find alternatives for energy supply. The aim of this project was to develop a technology in the treatment of SBP that would lead to savings in energy consumption and would provide a more competitive sugar production from sugar beets. These goals were met by the anaerobic digestion of SBP for biogas production. Lab-scale experiments confirmed the suitability of SBP as substrate for anaerobic bacteria. Pilot-scale experiments focused on process optimization and procedures for a quick start up and operational control. Both single-stage and two-stage process configurations showed similar removal efficiency. A stable biogas production could be achieved in single-stage at a maximum volumetric loading rate of 10 kgCSB/(m(3) x d). Degradation efficiency was 75% for VS and 72% for COD. Average specific gas production reached 530 NL/kgCOD(SBP) or 610 NL/kgVS(SBP). (CH(4): 50 to 53%). The first large-scale biogas plant was put into operation during the sugar processing period 2007 at a Hungarian sugar factory. Digesting approximately 50% of the SBP (800 t/d, 22%TS), the biogas produced could substitute about 40% of the natural gas required for the thermal energy supply within the sugar processing. Copyright IWA Publishing 2008.

  19. Molybdenum solubility in aluminium nitrate solutions

    Energy Technology Data Exchange (ETDEWEB)

    Heres, X.; Sans, D.; Bertrand, M.; Eysseric, C. [CEA, Centre de Marcoule, Nuclear Energy Division, DRCP, BP 17171, 30207 Bagnols-sur-Ceze Cedex (France); Brackx, E.; Domenger, R.; Excoffier, E. [CEA, Centre de Marcoule, Nuclear Energy Division, DTEC, BP 17171, 30207 Bagnols-sur-Ceze Cedex (France); Valery, J.F. [AREVA-NC, DOR/RDP, Paris - La Defense (France)

    2016-07-01

    For over 60 years, research reactors (RR or RTR for research testing reactors) have been used as neutron sources for research, radioisotope production ({sup 99}Mo/{sup 99m}Tc), nuclear medicine, materials characterization, etc... Currently, over 240 of these reactors are in operation in 56 countries. They are simpler than power reactors and operate at lower temperature (cooled to below 100 C. degrees). The fuel assemblies are typically plates or cylinders of uranium alloy and aluminium (U-Al) coated with pure aluminium. These fuels can be processed in AREVA La Hague plant after batch dissolution in concentrated nitric acid and mixing with UOX fuel streams. The aim of this study is to accurately measure the solubility of molybdenum in nitric acid solution containing high concentrations of aluminium. The higher the molybdenum solubility is, the more flexible reprocessing operations are, especially when the spent fuels contain high amounts of molybdenum. To be most representative of the dissolution process, uranium-molybdenum alloy and molybdenum metal powder were dissolved in solutions of aluminium nitrate at the nominal dissolution temperature. The experiments showed complete dissolution of metallic elements after 30 minutes long stirring, even if molybdenum metal was added in excess. After an induction period, a slow precipitation of molybdic acid occurs for about 15 hours. The data obtained show the molybdenum solubility decreases with increasing aluminium concentration. The solubility law follows an exponential relation around 40 g/L of aluminium with a high determination coefficient. Molybdenum solubility is not impacted by the presence of gadolinium, or by an increasing concentration of uranium. (authors)

  20. Acceptance of sugar reduction in flavored yogurt.

    Science.gov (United States)

    Chollet, M; Gille, D; Schmid, A; Walther, B; Piccinali, P

    2013-09-01

    To investigate what level of sugar reduction is accepted in flavored yogurt, we conducted a hedonic test focusing on the degree of liking of the products and on optimal sweetness and aroma levels. For both flavorings (strawberry and coffee), consumers preferred yogurt containing 10% added sugar. However, yogurt containing 7% added sugar was also acceptable. On the just-about-right scale, yogurt containing 10% sugar was more often described as too sweet compared with yogurt containing 7% sugar. On the other hand, the sweetness and aroma intensity for yogurt containing 5% sugar was judged as too low. A second test was conducted to determine the effect of flavoring concentration on the acceptance of yogurt containing 7% sugar. Yogurts containing the highest concentrations of flavoring (11% strawberry, 0.75% coffee) were less appreciated. Additionally, the largest percentage of consumers perceived these yogurts as "not sweet enough." These results indicate that consumers would accept flavored yogurts with 7% added sugar instead of 10%, but 5% sugar would be too low. Additionally, an increase in flavor concentration is undesirable for yogurt containing 7% added sugar. Copyright © 2013 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  1. Transcriptome and selected metabolite analyses reveal points of sugar metabolism in jackfruit (Artocarpus heterophyllus Lam.).

    Science.gov (United States)

    Hu, Lisong; Wu, Gang; Hao, Chaoyun; Yu, Huan; Tan, Lehe

    2016-07-01

    Artocarpus heterophyllus Lam., commonly known as jackfruit, produces the largest tree-borne fruit known thus far. The edible part of the fruit develops from the perianths, and contains many sugar-derived compounds. However, its sugar metabolism is poorly understood. A fruit perianth transcriptome was sequenced on an Illumina HiSeq 2500 platform, producing 32,459 unigenes with an average length of 1345nt. Sugar metabolism was characterized by comparing expression patterns of genes related to sugar metabolism and evaluating correlations with enzyme activity and sugar accumulation during fruit perianth development. During early development, high expression levels of acid invertases and corresponding enzyme activities were responsible for the rapid utilization of imported sucrose for fruit growth. The differential expression of starch metabolism-related genes and corresponding enzyme activities were responsible for starch accumulated before fruit ripening but decreased during ripening. Sucrose accumulated during ripening, when the expression levels of genes for sucrose synthesis were elevated and high enzyme activity was observed. The comprehensive transcriptome analysis presents fundamental information on sugar metabolism and will be a useful reference for further research on fruit perianth development in jackfruit. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  2. Conversion of yellow crude sugar into alcohol. Conversion of yellow crude sugar in a mixture with sugar beet molasses

    Energy Technology Data Exchange (ETDEWEB)

    Krishtul, F B; Malchenko, A L; Poluyanova, M T; Gromovich, V F; Maskimova, E A; Golodovskaya, A I; Pal' gova, L S

    1963-01-01

    Crude sugar (96.5 to 98.4% sucrose) in a mixture with molasses can be converted into alcohol by either batchwise or continuous fermentation processes with good process characteristics. Best yields are obtained when the amount of crude sugar is not more than 50% of the total weight of fermenting material. The bakers' yeast and alcohol produced are of good quality.

  3. Frontostriatal and behavioral adaptations to daily sugar-sweetened beverage intake: a randomized controlled trial.

    Science.gov (United States)

    Burger, Kyle S

    2017-03-01

    Background: Current obesity theories suggest that the repeated intake of highly palatable high-sugar foods causes adaptions in the striatum, parietal lobe, and prefrontal and visual cortices in the brain that may serve to perpetuate consumption in a feed-forward manner. However, the data for humans are cross-sectional and observational, leaving little ability to determine the temporal precedence of repeated consumption on brain response. Objective: We tested the impact of regular sugar-sweetened beverage intake on brain and behavioral responses to beverage stimuli. Design: We performed an experiment with 20 healthy-weight individuals who were randomly assigned to consume 1 of 2 sugar-sweetened beverages daily for 21 d, underwent 2 functional MRI sessions, and completed behavioral and explicit hedonic assessments. Results: Consistent with preclinical experiments, daily beverage consumption resulted in decreases in dorsal striatal response during receipt of the consumed beverage ( r = -0.46) and decreased ventromedial prefrontal response during logo-elicited anticipation ( r = -0.44). This decrease in the prefrontal response correlated with increases in behavioral disinhibition toward the logo of the consumed beverage ( r = 0.54; P = 0.02). Daily beverage consumption also increased precuneus response to both juice logos compared with a tasteless control ( r = 0.45), suggesting a more generalized effect toward beverage cues. Last, the repeated consumption of 1 beverage resulted in an explicit hedonic devaluation of a similar nonconsumed beverage ( P < 0.001). Conclusions: Analogous to previous reports, these initial results provide convergent data for a role of regular sugar-sweetened beverage intake in altering neurobehavioral responses to the regularly consumed beverage that may also extend to other beverage stimuli. Future research is required to provide evidence of replication in a larger sample and to establish whether the neurobehavioral adaptations observed

  4. Influence of gamma radiation on microbiological parameters of the ethanolic fermentation of sugar-cane must

    International Nuclear Information System (INIS)

    Alcarde, A.R.; Walder, J.M.M.; Horii, J.

    2003-01-01

    The influence of gamma radiation on reducing the population of some bacteria Bacillus and Lactobacillus that usually contaminate the sugar-cane must and its effects on acidity of the medium and viability of the yeast during fermentation were evaluated. The treatment with gamma radiation reduced the bacterial load of the sugar-cane must. Consequently, the volatile acidity produced during the fermentation of the must decreased and the viability of the yeast afterwards added increased

  5. APPLICATION OF FTA AND FMEA METHOD TO IMPROVE SUGAR PRODUCTION PROCESS QUALITY

    Directory of Open Access Journals (Sweden)

    JojoAndriana

    2016-10-01

    Full Text Available Defective product is a product that has poor quality and do not meet the standart. This defective products can give a bad impact to company, such as high production costs and decreased image company. Several methods that can be used to improve the quality is Six Sigma DMAIC methodology, FTA, and FMEA method. This study is conducted for several purpose, they are to determine the value of sigma level on the process of sugar production in PT.PG. Krebet Baru, to determine the factors that cause defective products in the process of sugar production by the FTA method, and to make a suitable solution based on the FMEA defective causes. The process sigma level in PT.PG.Krebet Baru is 3.58. That value sigma level indicates PT. PG. Krebet Baru is a company that are still growing and need improvement. The primary cause of the defects in the production process is a factor of operator and machine. Mode of failure with the highest RPN at 210 is time for steam process is too long, so they need to install the equipment that can detect the water level on sugar. When this equipment is installed, the exact time for drying will be known and the amount of defective product will be decreased.

  6. Cellulose hydrolysis by Trichoderma reesei cellulases: studies on adsorption, sugar production and synergism of cellobiohydrolase I,II and endoglucanase II

    Energy Technology Data Exchange (ETDEWEB)

    Medve, J.

    1997-02-01

    Three major cellulases have been purified by ion-exchange chromatography in an FPLC system. Microcrystalline cellulose (Avicel) was hydrolyzed by the single enzymes and by equimolar mixtures of CBH I-CBH II and CBH I-EG II. Enzyme adsorption was followed indirectly by selectively quantifying the enzymes in the supernatant by ion-exchange chromatography in an FPLC system. The (synergistic) production of small, soluble sugars (glucose, cellobiose and cellotriose) by the enzymes was followed by HPLC. 76 refs

  7. Kinetics of enzyme-catalyzed cross-linking of feruloylated arabinan from sugar beet

    DEFF Research Database (Denmark)

    Abang Zaidel, Dayang Norulfairuz; Arnous, Anis; Holck, Jesper

    2011-01-01

    the kinetics of HRP catalyzed cross-linking of FA esterified to α-(1,5)-linked arabinans are affected by the length of the arabinan chains carrying the feruloyl substitutions. The kinetics of the HRP-catalyzed cross-linking of four sets of arabinan samples from sugar beet pulp, having different molecular...... weights and hence different degrees of polymerization, were monitored by the disappearance of FA absorbance at 316 nm. MALDI-TOF/TOF-MS analysis confirmed that the sugar beet arabinans were feruloyl-substituted, and HPLC analysis verified that the amounts of diFAs increased when FA levels decreased...

  8. When Blood Sugar Is Too Low

    Science.gov (United States)

    ... Videos for Educators Search English Español When Blood Sugar Is Too Low KidsHealth / For Kids / When Blood ... get too low. The Causes of Low Blood Sugar Low blood sugar levels can happen to kids ...

  9. Solubility study of Tc(IV) oxides

    International Nuclear Information System (INIS)

    Liu, D.J.; Fan, X.H.

    2005-01-01

    solubility of Tc(IV) oxide decreases with the increase of pH at pH 10 and is pH independent in the range 2 3 2- concentration. These data could be used to estimate the Tc(IV) solubility for cases where solubility limits transport of technetium in reducing environments of high-level waste repositories. (authors)

  10. Effects of putrescine, kinetin and IAA on protein synthesis in 'Phaseolus vulgaris' coleoptiles

    Energy Technology Data Exchange (ETDEWEB)

    Crocomo, O J; Lee, T S.G. [Centro de Energia Nuclear na Agricultura, Piracicaba (Brazil)

    1975-01-01

    Incubation of etiolated 'Phaseolus vulgaris' coleoptiles shows a converse flux between soluble protein and reducing sugar. The rate of incorporation of radioactive arginine into protein was higher than that of radioactive leucine. Radioactive arginine incorporation into protein was linear up to 120 min and then started to decline. The rate of incorporation of radioactive leucine was increased by preincubation of the tissue in the incubation medium. Roots were found to contain more soluble protein and much less reducing sugar than the coleoptile. The optimum pH value for protein synthesis in coleoptile sections was found to be 6 for control tissues and 4 for those treated with 10-/sup 3/M IAA. This high concentration of IAA was also found to inhibit soluble protein synthesis, the incorporation rate of radioactive arginine and leucine into protein fraction, the secretion of hydrogen ion into the incubation medium and elongation of the bean segment. Kinetin at 2x10/sup -4/M and putrescine at 5mM both decreased the rate of /sup 14/C-arginine incorporation into soluble protein, but for /sup 14/C-leucine, this rate of incorporation was found to be increased after 90 min incubation with a preincubation of 30 min. In general, the change pattern of the soluble protein content, the reducing sugar level and the incorporation rate of radioactive arginine and leucine into protein in the kinetin and putrescine treated tissues were about the same although tissues that incubated with kinetin always contain more soluble protein and less reducing sugar than that of incubated with putrescine.

  11. The benefits of soluble non-bacterial fraction of kefir on blood pressure and cardiac hypertrophy in hypertensive rats are mediated by an increase in baroreflex sensitivity and decrease in angiotensin-converting enzyme activity.

    Science.gov (United States)

    Brasil, Girlandia Alexandre; Silva-Cutini, Mirian de Almeida; Moraes, Flávia de Souza Andrade; Pereira, Thiago de Melo Costa; Vasquez, Elisardo Corral; Lenz, Dominik; Bissoli, Nazaré Souza; Endringer, Denise Coutinho; de Lima, Ewelyne Miranda; Biancardi, Vinícia Campana; Maia, June Ferreira; de Andrade, Tadeu Uggere

    We aimed to evaluate whether long-term treatment with the soluble non-bacterial fraction of kefir affects mean arterial pressure (MAP) and cardiac hypertrophy through the modulation of baroreflex sensitivity, ACE activity, and the inflammatory-to-anti-inflammatory cytokine ratio in spontaneously hypertensive rats (SHRs). SHRs were treated with the soluble non-bacterial kefir fraction (SHR-kefir) or with kefir vehicle (SHR-soluble fraction of milk). Normotensive control Wistar Kyoto animals received the soluble fraction of milk. All treatments were administered by gavage (0.3 mL/100g/body weight), once daily for eight weeks. At the end, after basal MAP and Heart Rate (HT) measurement, barorreflex sensitivity was evaluated through in bolus administrations of sodium nitroprusside and phenylephrine (AP 50 [arterial pressure 50%], the lower plateau, and HR range were measured). ACE activity and cytokines (TNF-α and IL-10) were evaluated by ELISA. Cardiac hypertrophy was analysed morphometrically. Compared to SHR control, SHR-kefir exhibited a significant decrease in both MAP (SHR: 184 ± 5; SHR-Kefir: 142 ± 8 mmHg), and HR (SHR: 360 ± 10; SHR-kefir: 310 ± 14 bpm). The non-bacterial fraction of kefir also reduced cardiac hypertrophy, TNF-α-to-IL10 ratio, and ACE activity in SHRs. SHR-kefir baroreflex sensitivity, resulted in a partial but significant recovery of baroreflex gain, as demonstrated by improvements in AP 50 , the lower plateau, and HR range. In summary, our results indicate that long-term administration of the non-bacterial fraction of kefir promotes a significant decrease in both MAP and HR, by improving baroreflex, and reduces cardiac hypertrophy in SHRs, likely via ACE inhibition, and reduction of the TNF-α-to-IL10 ratio. Copyright © 2018 Elsevier Inc. All rights reserved.

  12. Aqueous solubility of calcium citrate and interconversion between the tetrahydrate and the hexahydrate as a balance between endothermic dissolution and exothermic complex formation

    DEFF Research Database (Denmark)

    Hedegaard, Martina Vavrusova; Skibsted, Leif Horsfelt

    2016-01-01

    Aqueous solubility of calcium citrate tetrahydrate was found to decrease with increasing temperature, while solubility of hexahydrate increased with a transition temperature at 51.6 °C. Excess citrate increased calcium citrate solubility but decreased the calcium ion activity of the saturated sol...

  13. Issues concerning the determination of solubility products of sparingly soluble crystalline solids. Solubility of HfO2(cr)

    International Nuclear Information System (INIS)

    Rai, Dhanpat; Kitamura, Akira; Rosso, Kevin M.; Sasaki, Takayuki; Kobayashi, Taishi

    2016-01-01

    Solubility studies were conducted with HfO 2 (cr) solid as a function HCl and ionic strength ranging from 2.0 to 0.004 mol kg -1 . These studies involved (1) using two different amounts of the solid phase, (2) acid washing the bulk solid phase, (3) preheating the solid phase to 1400 C, and (4) heating amorphous HfO 2 (am) suspensions to 90 C to ascertain whether the HfO 2 (am) converts to HfO 2 (cr) and to determine the solubility from the oversaturation direction. Based on the results of these treatments it is concluded that the HfO 2 (cr) contains a small fraction of less crystalline, but not amorphous, material [HfO 2 (lcr)] and this, rather than the HfO 2 (cr), is the solubility-controlling phase in the range of experimental variables investigated in this study. The solubility data are interpreted using both the Pitzer and SIT models and they provide log 10 K 0 values of -(59.75±0.35) and -(59.48±0.41), respectively, for the solubility product of HfO 2 (lcr)[HfO 2 (lcr) + 2H 2 O ↔ Hf 4+ + 4OH - ]. The log 10 of the solubility product of HfO 2 (cr) is estimated to be < -63. The observation of a small fraction of less crystalline higher solubility material is consistent with the general picture that mineral surfaces are often structurally and/or compositionally imperfect leading to a higher solubility than the bulk crystalline solid. This study stresses the urgent need, during interpretation of solubility data, of taking precautions to make certain that the observed solubility behavior for sparingly-soluble solids is assigned to the proper solid phase.

  14. Differential responses of sugar, organic acids and anthocyanins to source-sink modulation in Cabernet Sauvignon and Sangiovese grapevines.

    Science.gov (United States)

    Bobeica, Natalia; Poni, Stefano; Hilbert, Ghislaine; Renaud, Christel; Gomès, Eric; Delrot, Serge; Dai, Zhanwu

    2015-01-01

    Grape berry composition mainly consists of primary and secondary metabolites. Both are sensitive to environment and viticultural management. As a consequence, climate change can affect berry composition and modify wine quality and typicity. Leaf removal techniques can impact berry composition by modulating the source-to-sink balance and, in turn, may mitigate some undesired effects due to climate change. The present study investigated the balance between technological maturity parameters such as sugars and organic acids, and phenolic maturity parameters such as anthocyanins in response to source-sink modulation. Sugar, organic acid, and anthocyanin profiles were compared under two contrasting carbon supply levels in berries of cv. Cabernet Sauvignon and Sangiovese collected at 9 and 14 developmental stages respectively. In addition, whole-canopy net carbon exchange rate was monitored for Sangiovese vines and a mathematic model was used to calculate the balance between carbon fixation and berry sugar accumulation. Carbon limitation affected neither berry size nor the concentration of organic acids at harvest. However, it significantly reduced the accumulation of sugars and total anthocyanins in both cultivars. Most interestingly, carbon limitation decreased total anthocyanin concentration by 84.3% as compared to the non source-limited control, whereas it decreased sugar concentration only by 27.1%. This suggests that carbon limitation led to a strong imbalance between sugars and anthocyanins. Moreover, carbon limitation affected anthocyanin profiles in a cultivar dependent manner. Mathematical analysis of carbon-balance indicated that berries used a higher proportion of fixed carbon for sugar accumulation under carbon limitation (76.9%) than under carbon sufficiency (48%). Thus, under carbon limitation, the grape berry can manage the metabolic fate of carbon in such a way that sugar accumulation is maintained at the expense of secondary metabolites.

  15. Differential responses of sugar, organic acids and anthocyanins to source-sink modulation in Cabernet Sauvignon and Sangiovese grapevines

    Directory of Open Access Journals (Sweden)

    Natalia eBobeica

    2015-05-01

    Full Text Available Grape berry composition mainly consists of primary and secondary metabolites. Both are sensitive to environment and viticultural management. As a consequence, climate change can affect berry composition and modify wine quality and typicity. Leaf removal techniques can impact berry composition by modulating the source-to-sink balance and, in turn, may mitigate some undesired effects due to climate change. The present study investigated the balance between technological maturity parameters such as sugars and organic acids, and phenolic maturity parameters such as anthocyanins in response to source-sink modulation. Sugar, organic acid, and anthocyanin profiles were compared under two contrasting carbon supply levels in berries of cv. Cabernet Sauvignon and Sangiovese collected at 9 and 14 developmental stages respectively. In addition, whole-canopy net carbon exchange rate was monitored for Sangiovese vines and a mathematic model was used to calculate the balance between carbon fixation and berry sugar accumulation. Carbon limitation affected neither berry size nor the concentration of organic acids at harvest. However, it significantly reduced the accumulation of sugars and total anthocyanins in both cultivars. Most interestingly, carbon limitation decreased total anthocyanin concentration by 84.3 % as compared to the non source-limited control, whereas it decreased sugar concentration only by 27.1 %. This suggests that carbon limitation led to a strong imbalance between sugars and anthocyanins. Moreover, carbon limitation affected anthocyanin profiles in a cultivar dependent manner. Mathematical analysis of carbon-balance indicated that berries used a higher proportion of fixed carbon for sugar accumulation under carbon limitation (76.9% than under carbon sufficiency (48%. Thus, under carbon limitation, the grape berry can manage the metabolic fate of carbon in such a way that sugar accumulation is maintained at the expense of secondary

  16. The solubility of solid fission products in carbides and nitrides of uranium and plutonium: Pt.2. Solubility rules based on lattice parameter differences

    International Nuclear Information System (INIS)

    Benedict, U.

    1977-01-01

    The Relative Lattice Parameter Difference (RLPD) is defined for a solute element with respect to cubic carbides and nitrides of uranium and plutonium as solvents. Rules are given for the relationship between the solubility and the RLPD. NaCl type monocarbides with RLPD's from -10.2% to +7.8% are completely miscible with UC and PuC. NaCl type mononitrides with RLPD's from -7.5% to +8.5% are completely miscible with UN and PuN. The solubility in the sesquicarbides increases with decreasing RPLD and becomes complete in Pu 2 C 3 at RLPD = +4%, and in U 2 C 3 at RLPD approximately +1.5%. Solubilities are predicted on the basis of these rules for the cases where no experimental results are available

  17. The UK sugar tax - a healthy start?

    Science.gov (United States)

    Jones, C M

    2016-07-22

    The unexpected announcement by the UK Chancellor of the Exchequer of a levy on sugar sweetened beverages (SSBs) on the 16 March 2016, should be welcomed by all health professionals. This population based, structural intervention sends a strong message that there is no place for carbonated drinks, neither sugared nor sugar-free, in a healthy diet and the proposed levy has the potential to contribute to both general and dental health. The sugar content of drinks exempt from the proposed sugar levy will still cause tooth decay. Improving the proposed tax could involve a change to a scaled volumetric tax of added sugar with a lower exemption threshold. External influences such as the Common Agricultural Policy and the Transatlantic Trade and Investment Partnership may negate the benefits of the sugar levy unless it is improved. However, the proposed UK sugar tax should be considered as a start in improving the nation's diet.

  18. Graded levels of sugar syrup in broiler rations and its effect on growth performance and blood biochemical parameters

    Directory of Open Access Journals (Sweden)

    Ahmed S. Hussein

    2016-09-01

    Full Text Available Dietary energy for chickens normally includes cereal grains and fat. This innovative study investigated the effect of replacing part of the corn and fat in broiler chicken rations with graded levels of sugar syrup on growth performance and biochemical parameters. Experimental treatments consisted of feeding a corn-soy basal diet alone, or with graded levels of sugar syrup in increments of 5%, 10% and 15%. All starter diets were isonitrogenous and isocaloric. Body weight gain and efficiency of feed utilization of chicks fed the control diet alone were not significantly (P < 0.05 different from chicks fed diets supplemented with either 5% or 15% sugar syrup. Supplementation of sugar syrup to broiler diets had no significant effect on blood glucose, creatinine, total protein, or liver enzymes. Adding 5% sugar syrup to broiler rations significantly decreased blood cholesterol and triglycerides in chickens fed the sugar syrup diet compared with birds fed the control diet. In conclusion, the results shows sugar syrup can be used in poultry ration to replace part of the corn as a source of energy. These results allowed the authors to recommend the safe usage of sugar syrup in broiler rations.

  19. pH level, Ascorbic Acid, Proline and Soluble Sugar as Bio ...

    African Journals Online (AJOL)

    2017-08-12

    Aug 12, 2017 ... white blood cell, proline is a part of many protein and enzymes and has ..... cellular osmotic adjustment. Ashton and .... be attributed to increased respiration and decreased .... or industrial activities that took place, coupled with.

  20. Heat-induced alterations in cashew allergen solubility and IgE binding

    Directory of Open Access Journals (Sweden)

    Christopher P. Mattison

    Full Text Available Cashew nuts are an increasingly common cause of food allergy. We compare the soluble protein profile of cashew nuts following heating. SDS-PAGE indicate that heating can alter the solubility of cashew nut proteins. The 11S legumin, Ana o 2, dominates the soluble protein content in ready to eat and mildly heated cashew nuts. However, we found that in dark-roasted cashew nuts, the soluble protein profile shifts and the 2S albumin Ana o 3 composes up to 40% of the soluble protein. Analysis of trypsin-treated extracts by LC/MS/MS indicate changes in the relative number and intensity of peptides. The relative cumulative intensity of the 5 most commonly observed Ana o 1 and 2 peptides are altered by heating, while those of the 5 most commonly observed Ana o 3 peptides remaine relatively constant. ELISA experiments indicate that there is a decrease in rabbit IgG and human serum IgE binding to soluble cashew proteins following heating. Our findings indicate that heating can alter the solubility of cashew allergens, resulting in altered IgE binding. Our results support the use of both Ana o 2 and Ana o 3 as potential cashew allergen diagnostic targets. Keywords: Cashew nut, Food allergy, Immunoglobulin E, Mass-spectrometry, Peptide, Solubility

  1. Impact of the Sugar Import Reduction on Iran Economic Value Added (Input- Output Approach

    Directory of Open Access Journals (Sweden)

    Fateme Hayatgheibi

    2014-06-01

    Full Text Available The present study aimed at understanding interactions and linkages between the sugar sector with other economic sectors, and the influence of sugar import reduction on the economic value added. To achieve the purpose, the Input-Output table of Iran for the year 2006, Leontief inverse matrix and hypothetical extraction method were used. Based on the results, sugar industry has the most forward linkages with “Manufacture of food products and beverages,…”, “husbandry, aviculture,…”, “cultivation, horticulture”, “bakery products” and “restaurants”. This sector has also strong backward linkages with “cultivation, horticulture”, “chemicals and chemical products”, “other services”, “transport and telecommunication” and “financial services, insurance and bank”. Furthermore, either one unit increase in the final demand of sugar or one unit decrease in the sugar import increases the output of whole economic, agricultural and fishing, industry and mining, and services sectors by 2.3060, 0.6019, 1.4331, and 0.2710 unit, respectively. The increasing coefficients of the value added for the above sectors are 0.4308, 0.3700, and 0.1992 unit, respectively.

  2. High-Fat, High-Sugar Diet-Induced Subendothelial Matrix Stiffening is Mitigated by Exercise.

    Science.gov (United States)

    Kohn, Julie C; Azar, Julian; Seta, Francesca; Reinhart-King, Cynthia A

    2018-03-01

    Consumption of a high-fat, high-sugar diet and sedentary lifestyle are correlated with bulk arterial stiffening. While measurements of bulk arterial stiffening are used to assess cardiovascular health clinically, they cannot account for changes to the tissue occurring on the cellular scale. The compliance of the subendothelial matrix in the intima mediates vascular permeability, an initiating step in atherosclerosis. High-fat, high-sugar diet consumption and a sedentary lifestyle both cause micro-scale subendothelial matrix stiffening, but the impact of these factors in concert remains unknown. In this study, mice on a high-fat, high-sugar diet were treated with aerobic exercise or returned to a normal diet. We measured bulk arterial stiffness through pulse wave velocity and subendothelial matrix stiffness ex vivo through atomic force microscopy. Our data indicate that while diet reversal mitigates high-fat, high-sugar diet-induced macro- and micro-scale stiffening, exercise only significantly decreases micro-scale stiffness and not macro-scale stiffness, during the time-scale studied. These data underscore the need for both healthy diet and exercise to maintain vascular health. These data also indicate that exercise may serve as a key lifestyle modification to partially reverse the deleterious impacts of high-fat, high-sugar diet consumption, even while macro-scale stiffness indicators do not change.

  3. Influence of milling process on efavirenz solubility

    Directory of Open Access Journals (Sweden)

    Erizal Zaini

    2017-01-01

    Full Text Available Introduction: The aim of this study was to investigate the influence of the milling process on the solubility of efavirenz. Materials and Methods: Milling process was done using Nanomilling for 30, 60, and 180 min. Intact and milled efavirenz were characterized by powder X-ray diffraction, scanning electron microscopy (SEM, spectroscopy infrared (IR, differential scanning calorimetry (DSC, and solubility test. Results: The X-ray diffractogram showed a decline on peak intensity of milled efavirenz compared to intact efavirenz. The SEM graph depicted the change from crystalline to amorphous habit after milling process. The IR spectrum showed there was no difference between intact and milled efavirenz. Thermal analysis which performed by DSC showed a reduction on endothermic peak after milling process which related to decreasing of crystallinity. Solubility test of intact and milled efavirenz was conducted in distilled water free CO2with 0.25% sodium lauryl sulfate media and measured using high-performance liquid chromatography method with acetonitrile: distilled water (80:20 as mobile phases. The solubility was significantly increased (P < 0.05 after milling processes, which the intact efavirenz was 27.12 ± 2.05, while the milled efavirenz for 30, 60, and 180 min were 75.53 ± 1.59, 82.34 ± 1.23, and 104.75 ± 0.96 μg/mL, respectively. Conclusions: Based on the results, the solubility of efavirenz improved after milling process.

  4. Calcination of Fluorinel-sodium waste blends using sugar as a feed additive (formerly WINCO-11879)

    International Nuclear Information System (INIS)

    Newby, B.J.; Thomson, T.D.; O'Brien, B.H.

    1992-06-01

    Methods were studied for using sugar as a feed additive for converting the sodium-bearing wastes stored at the Idaho Chemical Processing Plant into granular, free flowing solids by fluidized-bed calcination at 500 degrees C. All methods studied blended sodium-bearing wastes with Fluorinel wastes but differed in the types of sugar (sucrose or dextrose) that were added to the blend. The most promising sugar additive was determined to be sucrose, since it is converted more completely to inorganic carbon than is dextrose. The effect of the feed aluminum-to-alkali metal mole ratio on calcination of these blends with sugar was also investigated. Increasing the aluminum-to-alkali metal ratio from 0.6 to 1.0 decreased the calcine product-to-fines ratio from 3.0 to 1.0 and the attrition index from 80 to 15%. Further increasing the ratio to 1.25 had no effect

  5. Gradual reduction of free sugars in beverages on sale by implementing the beverage checklist as a public health strategy.

    Science.gov (United States)

    Luger, Maria; Winzer, Eva; Schätzer, Manuel; Dämon, Sabine; Moser, Nadine; Blagusz, Karin; Rittmannsberger, Barbara; Schätzer, Julia; Lechleitner, Monika; Rieder, Anita; Hoppichler, Friedrich

    2018-03-15

    Sugar-sweetened beverages (SSBs) are a major source of free sugar intake and contribute to obesity and obesity-related diseases. Therefore, we analyzed the effect of a gradual sugar reduction strategy within the so-called 'beverage checklist' on free sugar content in beverages on sale in Austria. From 2010 until 2017, data on the amount of free sugar of sweetened beverages (sweetened with sugars, fruit juice and artificial sweeteners) with 0.20-0.75l serving sizes in all main supermarkets and from industry was collected. These data were published annually as the beverage checklist, which displays beverages on sale in Austria. The checklist aims to encourage beverage production with a free sugar content of ≤7.4 g/100 ml and no artificial sweeteners. Free sugar content in the total supply decreased significantly [7.53 (2.86) vs. 6.75 (2.79) g/100 ml; 10.4%; P strategy, conducted by a small non-profit organization, showed a reduction in the mean free sugar content by working with the industry to voluntarily reformulate beverages. More beverages with less added sugar were brought to the market, which implies healthier choices. The challenge now is to further engage the industry and also policy makers to achieve a greater reduction in the future.

  6. 3D printing PLA and silicone elastomer structures with sugar solution support material

    Science.gov (United States)

    Hamidi, Armita; Jain, Shrenik; Tadesse, Yonas

    2017-04-01

    3D printing technology has been used for rapid prototyping since 1980's and is still developing in a way that can be used for customized products with complex design and miniature features. Among all the available 3D printing techniques, Fused Deposition Modeling (FDM) is one of the most widely used technologies because of its capability to build different structures by employing various materials. However, complexity of parts made by FDM is greatly limited by restriction of using support materials. Support materials are often used in FDM for several complex geometries such as fully suspended shapes, overhanging surfaces and hollow features. This paper describes an approach to 3D print a structure using silicone elastomer and polylactide fiber (PLA) by employing a novel support material that is soluble in water. This support material is melted sugar which can easily be prepared at a low cost. Sugar is a carbohydrate, which is found naturally in plants such as sugarcane and sugar beets; therefore, it is completely organic and eco-friendly. As another advantage, the time for removing this material from the part is considerably less than other commercially available support materials and it can be removed easily by warm water without leaving any trace. Experiments were done using an inexpensive desktop 3D printer to fabricate complex structures for use in soft robots. The results envision that further development of this system would contribute to a method of fabrication of complex parts with lower cost yet high quality.

  7. Changes in Intakes of Total and Added Sugar and their Contribution to Energy Intake in the U.S.

    Directory of Open Access Journals (Sweden)

    Won O. Song

    2010-08-01

    Full Text Available This study was designed to document changes in total sugar intake and intake of added sugars, in the context of total energy intake and intake of nutrient categories, between the 1970s and the 1990s, and to identify major food sources contributing to those changes in intake. Data from the NHANES I and III were analyzed to obtain nationally representative information on food consumption for the civilian, non-institutionalized population of the U.S. from 1971 to 1994. In the past three decades, in addition to the increase in mean intakes of total energy, total sugar, added sugars, significant increases in the total intake of carbohydrates and the proportion of carbohydrates to the total energy intake were observed. The contribution of sugars to total carbohydrate intake decreased in both 1–18 y and 19+ y age subgroups, and the contribution of added sugars to the total energy intake did not change. Soft drinks/fluid milk/sugars and cakes, pastries, and pies remained the major food sources for intake of total sugar, total carbohydrates, and total energy during the past three decades. Carbonated soft drinks were the most significant sugar source across the entire three decades. Changes in sugar consumption over the past three decades may be a useful specific area of investigation in examining the effect of dietary patterns on chronic diseases.

  8. Serum concentrations of soluble Flt-1 are decreased among women with a viable fetus and no symptoms of miscarriage destined for pregnancy loss.

    Directory of Open Access Journals (Sweden)

    Tu'uhevaha J Kaitu'u-Lino

    Full Text Available Miscarriage is the most common complication of pregnancy. Pre-clinical miscarriage has an estimated incidence of 30%, whilst clinical miscarriage has an incidence of 12-15%. Two thirds of pregnancies lost to miscarriage are believed to be attributable to defective placentation, thus a number of studies have sought to identify markers of defective placentation that could be used as clinical biomarkers of miscarriage. Decreased soluble FMS-like tyrosine kinase-1 (sFlt1, placental growth factor (PlGF, and soluble endoglin (sEng in the maternal circulation during the first trimester have recently been proposed as potential markers of pregnancy loss. However, in these studies clinical samples were only obtained once women had presented with symptoms of miscarriage. In this study we prospectively screened serum samples collected from asymptomatic women with a viable fetus. We assessed maternal serum levels of sFlt1, PlGF and sEng across the first trimester of normal pregnancy and compared levels between women who continued to a live birth, to those who subsequently miscarried. Both sFlt1 and PlGF significantly (p≤0.05 increased across gestation in normal pregnancy with serum levels rising from 0.65±0.12 ng/ml at 6 weeks to 1.85±0.24 ng/ml at 12 weeks for sFlt1, and 57.2±19.2 pg/ml to 106±22.7 pg/ml for PlGF. sEng remained unchanged throughout the the first trimester. Importantly we detected a significant (35%, p≤0.05 decrease in sFlt1 levels between our control and miscarriage cohort, however there was significant overlap between cases and controls, suggesting serum sFlt1 is unlikely to be useful as a clinical biomarker in asymptomatic women. Nevertheless, our data suggests a dysregulation of angiogenic factors may be involved in the pathophysiology of miscarriage.

  9. The solubilities and solubility products of zirconium hydroxide and oxide after aging at 278, 313, and 333 K

    Energy Technology Data Exchange (ETDEWEB)

    Kobayashi, Taishi; Uemura, Takuya; Sasaki, Takayuki; Takagi, Ikuji [Kyoto Univ. (Japan). Dept. of Nuclear Engineering; Moriyama, Hirotake [Kyoto Univ. (Japan). Research Reactor Inst.

    2016-07-01

    The solubilities of zirconium hydroxide and oxide after aging at 278, 313, and 333 K were measured at 278, 298, 313, and 333 K in the pH{sub c} range of 0.3-7 in a 0.5 M ionic strength solution of NaClO{sub 4} and HClO{sub 4}. Size distributions of the colloidal species were investigated by ultrafiltration using membranes with different pore sizes, and the solid phases were examined by X-ray diffraction. The apparent solubility of zirconium amorphous hydroxide (Zr(OH){sub 4}(am)), prepared by the oversaturation method, decreased with increasing aging temperature (T{sub a}), and the size distributions obtained after aging at elevated temperatures indicated the growth of the colloidal species. We, therefore, suggested that agglomeration of the colloidal species and dehydration and crystallization of Zr(OH){sub 4}(am) as the solubility-limiting solid phase occurred over the course of aging at elevated temperatures. For sample solutions of the crystalline oxide (ZrO{sub 2}(cr)), the aging temperature had no significant effect on the solubility, but the solubility data at lower temperatures were found to be slightly higher than those at higher temperatures, implying a small fraction of the amorphous components. In the analysis of different solid phases (Zr(OH){sub 4}(s,T{sub a}), T{sub a} = 278, 313, and 333 K) depending on the aging temperatures, the solubility products (K{sub sp}, T{sub a}) were determined at different measurement temperatures, and the enthalpy change (Δ{sub r}H {sup circle}) for Zr{sup 4+} 4OH{sup -} <=> Zr(OH){sub 4}(s,T{sub a}) was calculated using the van't Hoff equation. The solid-phase-transformation process at elevated temperatures was also analyzed based on the obtained K{sub sp}, T{sub a} and Δ{sub r}H {sup circle} values.

  10. Solubility studies of Np(V) in simulated underground water

    International Nuclear Information System (INIS)

    Zhang Yingjie; Ren Lilong; Jiao Haiyang; Yao Jun; Su Xiguang; Fan Xianhua

    2004-01-01

    The solubility of Np(V) in simulated underground water has been measured with the variation of pH, storage time (0-100 days). All experiments were performed in an Ar glove box which contained high purity Ar, with an oxygen content of less than 5ppm. Experimental results show that the solubility of Np(V) in simulated underground water decreased with increasing pH value of solution; the solubility of Np(V) in simulated underground water determined at different pH is : pH=6.96, [Np(V)]=(3.52±0.37) x 10 -4 mol/L; pH=8.04, [Np(V)]=(8.24±0.32) x 10 -5 mol/L; pH=9.01, [Np(V)]=(3.04±0.48) x 10'- 5 mol/L, respectively. (author)

  11. Characterization of major ripening events during softening in grape: turgor, sugar accumulation, abscisic acid metabolism, colour development, and their relationship with growth.

    Science.gov (United States)

    Castellarin, Simone D; Gambetta, Gregory A; Wada, Hiroshi; Krasnow, Mark N; Cramer, Grant R; Peterlunger, Enrico; Shackel, Kenneth A; Matthews, Mark A

    2016-02-01

    Along with sugar accumulation and colour development, softening is an important physiological change during the onset of ripening in fruits. In this work, we investigated the relationships among major events during softening in grape (Vitis vinifera L.) by quantifying elasticity in individual berries. In addition, we delayed softening and inhibited sugar accumulation using a mechanical growth-preventing treatment in order to identify processes that are sugar and/or growth dependent. Ripening processes commenced on various days after anthesis, but always at similarly low elasticity and turgor. Much of the softening occurred in the absence of other changes in berry physiology investigated here. Several genes encoding key cell wall-modifying enzymes were not up-regulated until softening was largely completed, suggesting softening may result primarily from decreases in turgor. Similarly, there was no decrease in solute potential, increase in sugar concentration, or colour development until elasticity and turgor were near minimum values, and these processes were inhibited when berry growth was prevented. Increases in abscisic acid occurred early during softening and in the absence of significant expression of the V. vinifera 9-cis-epoxycarotenoid dioxygenases. However, these increases were coincident with decreases in the abscisic acid catabolite diphasic acid, indicating that initial increases in abscisic acid may result from decreases in catabolism and/or exogenous import. These data suggest that softening, decreases in turgor, and increases in abscisic acid represent some of the earliest events during the onset of ripening. Later, physical growth, further increases in abscisic acid, and the accumulation of sugar are integral for colour development. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  12. Intake of added sugar in Malaysia: a review.

    Science.gov (United States)

    Amarra, Maria Sofia V; Khor, Geok Lin; Chan, Pauline

    2016-01-01

    The term 'added sugars' refers to sugars and syrup added to foods during processing or preparation, and sugars and syrups added at the table. Calls to limit the daily intakes of added sugars and its sources arose from evidence analysed by WHO, the American Heart Association and other organizations. The present review examined the best available evidence regarding levels of added sugar consumption among different age and sex groups in Malaysia and sources of added sugars. Information was extracted from food balance sheets, household expenditure surveys, nutrition surveys and published studies. Varying results emerged, as nationwide information on intake of sugar and foods with added sugar were obtained at different times and used different assessment methods. Data from the 2003 Malaysian Adult Nutrition Survey (MANS) using food frequency questionnaires suggested that on average, Malaysian adults consumed 30 grams of sweetened condensed milk (equivalent to 16 grams sugar) and 21 grams of table sugar per day, which together are below the WHO recommendation of 50 grams sugar for every 2000 kcal/day to reduce risk of chronic disease. Published studies suggested that, for both adults and the elderly, frequently consumed sweetened foods were beverages (tea or coffee) with sweetened condensed milk and added sugar. More accurate data should be obtained by conducting population-wide studies using biomarkers of sugar intake (e.g. 24-hour urinary sucrose and fructose excretion or serum abundance of the stable isotope 13C) to determine intake levels, and multiple 24 hour recalls to identify major food sources of added sugar.

  13. EFFECT OF HEAT TREATMENT ON SOYBEAN PROTEIN SOLUBILITY

    Directory of Open Access Journals (Sweden)

    RODICA CĂPRIŢĂ

    2007-05-01

    Full Text Available The use of soybean products in animal feeds is limited due to the presence of antinutritional factors (ANF. Proper heat processing is required to destroy ANF naturally present in raw soybeans and to remove solvent remaining from the oil extraction process. Over and under toasting of soybean causes lower nutritional value. Excessive heat treatment causes Maillard reaction which affects the availability of lysine in particular and produces changes to the chemical structure of proteins resulting in a decrease of the nutritive value. The objective of this study was to evaluate the effect of heating time on the protein solubility. The investigation of the heating time on protein solubility in soybean meal (SBM revealed a negative correlation (r = -0.9596. Since the urease index is suitable only for detecting under processed SBM, the protein solubility is an important index for monitoring SBM quality.

  14. Issues concerning the determination of solubility products of sparingly soluble crystalline solids. Solubility of HfO{sub 2}(cr)

    Energy Technology Data Exchange (ETDEWEB)

    Rai, Dhanpat [Rai Enviro-Chem, LLC, Yachats, OR (United States); Kitamura, Akira [Japan Atomic Energy Agency, Ibaraki (Japan); Rosso, Kevin M. [Pacific Northwest National Laboratory, Richland, WA (United States); Sasaki, Takayuki; Kobayashi, Taishi [Kyoto Univ. (Japan)

    2016-11-01

    Solubility studies were conducted with HfO{sub 2}(cr) solid as a function HCl and ionic strength ranging from 2.0 to 0.004 mol kg{sup -1}. These studies involved (1) using two different amounts of the solid phase, (2) acid washing the bulk solid phase, (3) preheating the solid phase to 1400 C, and (4) heating amorphous HfO{sub 2}(am) suspensions to 90 C to ascertain whether the HfO{sub 2}(am) converts to HfO{sub 2}(cr) and to determine the solubility from the oversaturation direction. Based on the results of these treatments it is concluded that the HfO{sub 2}(cr) contains a small fraction of less crystalline, but not amorphous, material [HfO{sub 2}(lcr)] and this, rather than the HfO{sub 2}(cr), is the solubility-controlling phase in the range of experimental variables investigated in this study. The solubility data are interpreted using both the Pitzer and SIT models and they provide log{sub 10} K{sup 0} values of -(59.75±0.35) and -(59.48±0.41), respectively, for the solubility product of HfO{sub 2}(lcr)[HfO{sub 2}(lcr) + 2H{sub 2}O ↔ Hf{sup 4+} + 4OH{sup -}]. The log{sub 10} of the solubility product of HfO{sub 2}(cr) is estimated to be < -63. The observation of a small fraction of less crystalline higher solubility material is consistent with the general picture that mineral surfaces are often structurally and/or compositionally imperfect leading to a higher solubility than the bulk crystalline solid. This study stresses the urgent need, during interpretation of solubility data, of taking precautions to make certain that the observed solubility behavior for sparingly-soluble solids is assigned to the proper solid phase.

  15. 27 CFR 24.181 - Use of sugar.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Use of sugar. 24.181... OF THE TREASURY LIQUORS WINE Production of Wine § 24.181 Use of sugar. Only sugar, as defined in § 24.10, may be used in the production of standard wine. The quantity of sugar used will be determined...

  16. Soluble Non-ammonia Nitrogen in Ruminal and Omasal Digesta of Korean Native Steers Supplemented with Soluble Proteins

    Directory of Open Access Journals (Sweden)

    C. W. Choi

    2012-09-01

    Full Text Available An experiment was conducted to study the effect of soluble protein supplements on concentration of soluble non-ammonia nitrogen (SNAN in the liquid phase of ruminal (RD and omasal digesta (OD of Korean native steers, and to investigate diurnal pattern in SNAN concentration in RD and OD. Three ruminally cannulated Korean native steers in a 3×3 Latin square design consumed a basal diet of rice straw and corn-based concentrate (control, and that supplemented (kg/d DM basis with intact casein (0.24; IC or acid hydrolyzed casein (0.46; AHC. Ruminal digesta was sampled using a vacuum pump, whereas OD was collected using an omasal sampling system at 2.0 h intervals after a morning feeding. The SNAN fractions (free amino acid (AA, peptide and soluble protein in RD and OD were assessed using the ninhydrin assay. Concentrations of free AA and total SNAN in RD were significantly (p<0.05 lower than those in OD. Although free AA concentration was relatively high, mean peptide was quantitatively the most important fraction of total SNAN in both RD and OD, indicating that degradation of peptide to AA rather than hydrolysis of soluble protein to peptide or deamination may be the most limiting step in rumen proteolysis of Korean native steers. Diurnal variation in peptide concentration in OD for the soluble protein supplemented diets during the feeding cycle peaked 2 h post-feeding and decreased thereafter whereas that for the control was relatively constant during the entire feeding cycle. Diurnal variation in peptide concentration was rather similar between RD and OD.

  17. Frontostriatal and behavioral adaptations to daily sugar-sweetened beverage intake: a randomized controlled trial123

    Science.gov (United States)

    2017-01-01

    Background: Current obesity theories suggest that the repeated intake of highly palatable high-sugar foods causes adaptions in the striatum, parietal lobe, and prefrontal and visual cortices in the brain that may serve to perpetuate consumption in a feed-forward manner. However, the data for humans are cross-sectional and observational, leaving little ability to determine the temporal precedence of repeated consumption on brain response. Objective: We tested the impact of regular sugar-sweetened beverage intake on brain and behavioral responses to beverage stimuli. Design: We performed an experiment with 20 healthy-weight individuals who were randomly assigned to consume 1 of 2 sugar-sweetened beverages daily for 21 d, underwent 2 functional MRI sessions, and completed behavioral and explicit hedonic assessments. Results: Consistent with preclinical experiments, daily beverage consumption resulted in decreases in dorsal striatal response during receipt of the consumed beverage (r = −0.46) and decreased ventromedial prefrontal response during logo-elicited anticipation (r = −0.44). This decrease in the prefrontal response correlated with increases in behavioral disinhibition toward the logo of the consumed beverage (r = 0.54; P = 0.02). Daily beverage consumption also increased precuneus response to both juice logos compared with a tasteless control (r = 0.45), suggesting a more generalized effect toward beverage cues. Last, the repeated consumption of 1 beverage resulted in an explicit hedonic devaluation of a similar nonconsumed beverage (P < 0.001). Conclusions: Analogous to previous reports, these initial results provide convergent data for a role of regular sugar-sweetened beverage intake in altering neurobehavioral responses to the regularly consumed beverage that may also extend to other beverage stimuli. Future research is required to provide evidence of replication in a larger sample and to establish whether the neurobehavioral adaptations observed

  18. Inorganic elements in sugar samples

    International Nuclear Information System (INIS)

    Salles, Paulo M.B. de; Campos, Tarcisio P.R. de

    2013-01-01

    Sugar is considered a safe food ingredient; however, it can be contaminated by organic elements since its planting until its production process. Thus, this study aims at checking the presence of inorganic elements in samples of crystal, refined and brown sugar available for consumption in Brazil. The applied technique was neutron activation analysis, the k 0 method, using the TRIGA MARK - IPR-R1 reactor located at CDTN/CNEN, in Belo Horizonte. It was identified the presence of elements such as, Au, Br, Co, Cr, Hf, K, Na, Sb, Sc and Zn in the samples of crystal/refined sugar and the presence of As, Au, Br, Ca, Co, Cr, Cs, Fe, Hf, K, Na, Sb, Sc, Sm, Sr, Th and Zn in the brown sugar samples. The applied technique was appropriate to this study because it was not necessary to put the samples in solution, essential condition in order to apply other techniques, avoiding contaminations and sample losses, besides allowing a multi elementary detection in different sugar samples. (author)

  19. Inorganic elements in sugar samples

    Energy Technology Data Exchange (ETDEWEB)

    Salles, Paulo M.B. de; Campos, Tarcisio P.R. de, E-mail: pauladesalles@yahoo.com.br, E-mail: tprcampos@pq.cnpq.br [Universidade Federal de Minas Gerais (DEN/UFMG), Belo Horizonte, MG (Brazil). Departamento de Engenharia Nuclear; Menezes, Maria Angela de B.C., E-mail: menezes@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)

    2013-07-01

    Sugar is considered a safe food ingredient; however, it can be contaminated by organic elements since its planting until its production process. Thus, this study aims at checking the presence of inorganic elements in samples of crystal, refined and brown sugar available for consumption in Brazil. The applied technique was neutron activation analysis, the k{sub 0} method, using the TRIGA MARK - IPR-R1 reactor located at CDTN/CNEN, in Belo Horizonte. It was identified the presence of elements such as, Au, Br, Co, Cr, Hf, K, Na, Sb, Sc and Zn in the samples of crystal/refined sugar and the presence of As, Au, Br, Ca, Co, Cr, Cs, Fe, Hf, K, Na, Sb, Sc, Sm, Sr, Th and Zn in the brown sugar samples. The applied technique was appropriate to this study because it was not necessary to put the samples in solution, essential condition in order to apply other techniques, avoiding contaminations and sample losses, besides allowing a multi elementary detection in different sugar samples. (author)

  20. Solubility and diffusion coefficient of oxygen in silicon

    International Nuclear Information System (INIS)

    Itoh, Yoshiko; Nozaki, Tadashi

    1985-01-01

    The solubility and diffusion coefficient of oxygen in silicon between 1000 0 C and 1375 0 C were examined by charged particle activation analysis with the 16 O( 3 He,p) 18 F reaction, in which oxygen was activated with an equal probability over the depth of up to 250μm by a specially devised apparatus. Silicon wafers of known histories were heated in oxygen or argon for 12 to 473 hours, and the resultant oxygen depth profiles were determined by the activation, subsequent stepwise etching and 18 F activity measurement. The solubility thus obtained is given as 9.3 x 10 21 exp[-27.6kcal mol -1 /RT] at.cm -3 ; the diffusion coefficient has been found to be approximated as 3.2 exp[-67.1kcal mol -1 /RT] cm 2 s -1 over 1150 0 C, under which the apparent activation energy seems to decrease with decrease of temperature. (author)

  1. Mechanical and thermal properties of environmentally friendly composites derived from sugar palm tree

    International Nuclear Information System (INIS)

    Sahari, J.; Sapuan, S.M.; Zainudin, E.S.; Maleque, M.A.

    2013-01-01

    Highlights: ► We successfully developed biocomposites derived from sugar palm tree. ► The addition of SPF improve the mechanical properties of biocomposites. ► The thermal stability of biocomposites increase with increasing of SPF. ► The water absorption of biocomposites decrease with increasing of SPF. ► We investigate the morphological fracture through scanning electron microscopy. - Abstract: The aim of this paper is to study the effect of fibre content on mechanical properties, water absorption behaviour and thermal properties of sugar palm fibre (SPF) reinforced plasticized sugar palm starch (SPF/SPS) biocomposites. The biocomposites were prepared with different amounts of fibres (i.e. 10%, 20% and 30% by weight percent) by using glycerol as plasticizer for the starch. The mechanical properties of plasticized SPS improved with the incorporation of fibres. Fibre loading also increased the thermal stability of the biocomposite in this investigation. Water uptake and moisture content of SPF/SPS biocomposites decreased with the incorporation of fibres, which is due to better interfacial bonding between the matrix and fibres as well as the hindrance to absorption caused by the fibres. Fractographic studies through scanning electron microscopy showed homogeneous distribution of fibres and matrix with good adhesion which play an important role in improving the mechanical properties of biocomposites

  2. Textual analysis of sugar industry influence on the World Health Organization’s 2015 sugars intake guideline

    Science.gov (United States)

    Reeves, Aaron; Loopstra, Rachel; McKee, Martin

    2016-01-01

    Abstract Objective To determine whether sugar industry-related organizations influenced textual changes between the draft and final versions of the World Health Organization’s (WHO’s) 2015 guideline Sugars intake for adults and children. Methods Stakeholder consultation submissions on the draft guideline from seven sugar industry-related and 10 public health organizations were assessed using the Wordscores program. Document scores were rescaled using the Martin–Vanberg transformation to improve comparability. Draft and final guidelines were compared to identify changes influenced by the sugar industry and public health organizations. Findings There was a small shift in transformed Wordscores score between the draft and final guidelines, from 0.25 to 0.24, towards the industry position. The change was linked to increased use of the word “low” to describe the quality of the evidence, consistent with industry arguments. There was also a shift from use of the word “consumption” to “intake”, irrespective of policy position. Scores for World Sugar Research Organisation and Sugar Nutrition UK submissions ( 0.11 and 0.18, respectively) represented strong pro-industry positions and scores for European Public Health Alliance and Wemos submissions (1.00 and 0.88, respectively) represented the strongest public health positions. Industry tactics included challenging the quality of the evidence, distinguishing between different types of sugar and advocating harm reduction. Conclusion There was little change between draft and final versions of the WHO sugars intake guideline 2015, following industry consultation. The main change was linked to emphasizing the low quality of the evidence on sugar’s adverse effects. Guideline development appeared relatively resistant to industry influence at the stakeholder consultation stage. PMID:27516634

  3. Detection of colloidal silver chloride near solubility limit

    Science.gov (United States)

    Putri, K. Y.; Adawiah, R.

    2018-03-01

    Detection of nanoparticles in solution has been made possible by several means; one of them is laser-induced breakdown detection (LIBD). LIBD is able to distinguish colloids of various sizes and concentrations. This technique has been used in several solubility studies. In this study, the formation of colloids in a mixed system of silver nitrate and sodium chloride was observed by acoustic LIBD. Silver chloride has low solubility limit, therefore LIBD measurement is appropriate. Silver and chloride solutions with equal concentrations, set at below and above the solubility of silver chloride as the expected solid product, were mixed and the resulting colloids were observed. The result of LIBD measurement showed that larger particles were present as more silver and chloride introduced. However, once the concentrations exceeded the solubility limit of silver chloride, the detected particle size seemed to be decreasing, hence suggested the occurrence of coprecipitation process. This phenomenon indicated that the ability of LIBD to detect even small changes in colloid amounts might be a useful tool in study on formation and stability of colloids, i.e. to confirm whether nanoparticles synthesis has been successfully performed and whether the system is stable or not.

  4. Added sugars in kids' meals from chain restaurants

    OpenAIRE

    Scourboutakos, Mary J.; Semnani-Azad, Zhila; L'Abbé, Mary R.

    2016-01-01

    Objective To analyze the added sugars in kids' meals from Canadian chain restaurants in relation to the World Health Organization's proposed sugar recommendation (less than 5% of total daily calories should come from added sugars) and current recommendation (less than 10% of total daily calories should come from added sugars). Methods Total sugar levels were retrieved from the websites of 10 fast-food and 7 sit-down restaurants in 2010. The added sugar levels in 3178 kids' meals from Canadian...

  5. 27 CFR 24.317 - Sugar record.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Sugar record. 24.317... OF THE TREASURY LIQUORS WINE Records and Reports § 24.317 Sugar record. A proprietor who receives, stores, or uses sugar shall maintain a record of receipt and use. The record will show the date of...

  6. The Solubility of metal oxides in molten carbonates - why the acid-basic chemistry fails?

    DEFF Research Database (Denmark)

    Bjerrum, Niels; Qingfeng, Li; Borup, Flemming

    1999-01-01

    Solubilities of various metal oxides in molten Li/K carbonates have been measured at 650°C under carbon dioxide atmosphere. It is found that the solubility of NiO and PbO decreases with increasing lithium mole fraction and decreasing CO2 partial pressure. On the other hand, the emf measurement...... shows opposite effects, i.e., decreasing CO2 pressure leads to more negative emf values but increasing lithium content gives more positive emf values. This contradiction is explained by means of a complex formation model. The possible species for lead are proposed to be [Pb(CO3)2]-2 and/or [Pb(CO3) 3...

  7. Mechanical properties, phenolic composition and extractability indices of Barbera grapes of different soluble solids contents from several growing areas.

    Science.gov (United States)

    Torchio, Fabrizio; Cagnasso, Enzo; Gerbi, Vincenzo; Rolle, Luca

    2010-02-15

    Phenolic compounds, extractable from grape skins and seeds, have a notable influence on the quality of red wines. Many studies have clearly demonstrated the relationship between the phenolic composition of the grape at harvest time and its influence on the phenolic composition of the red wine produced. In many previous works the evolution of phenolic composition and relative extractability was normally studied on grapes sampled at different times during ripening, but at the same date the physiological characteristics of grape berries in a vineyard are often very heterogeneous. Therefore, the main goal of the study is to investigate the differences among mechanical properties, phenolic composition and relative extractability of Vitis vinifera L. cv Barbera grape berries, harvested at the same date from several vineyards, and calibrated according to their density at three levels of soluble solids (A=235+/-8, B=252+/-8 and C=269+/-8 g L(-1) sugar) with the aim of studying the influence of ripeness stages and growing locations on these parameters. Results on mechanical properties showed that the thickness of the berry skin (Sp(sk)) was the parameter most affected by the different level of sugars in the pulp, while different skin hardnesses, evaluated by the break skin force (F(sk)), were related to the cultivation sites. The latter were also observed to influence the mechanical characteristics of seeds. Generally, the anthocyanin content increased with the level of soluble solids, while the increase in the tannin content of the berry skin and seeds was less marked. However, significant changes in flavanols reactive to vanillin in the seeds were found. The cellular maturity index (EA%) was little influenced by the soluble solids content of grapes. Copyright 2009 Elsevier B.V. All rights reserved.

  8. Safety assessment of sugar dusting treatments by analysis of hygienic behavior in honey bee colonies

    Directory of Open Access Journals (Sweden)

    Stevanovic Jevrosima

    2011-01-01

    Full Text Available The hygienic behavior in honey bees is a dominant natural defense mechanism against brood diseases. In this study, the influence of sugar dusting treatments on hygienic behavior was evaluated in 44 strong honey bee colonies. Three doses of pulverized sugar, 20, 30 and 40 g, each applied at three-, seven- and fourteen-day intervals were tested. The percentage of cleaned cells (PCC in the total number of those with pin-killed brood served as a measure of the hygienic potential. The effect was dependent on the frequency of treatments: all doses applied every third and seventh day significantly (p<0.001 decreased the PCC in comparison with the untreated control colonies. Nevertheless, sugar did not threaten the hygienic potential, as PPC values remained above 94% following all treatments. Thus, it can be concluded that the tested sugar treatments are safe and can be justifiably implemented into integrated pest management strategies to control Varroa destructor.

  9. Gas solubilities widespread applications

    CERN Document Server

    Gerrard, William

    1980-01-01

    Gas Solubilities: Widespread Applications discusses several topics concerning the various applications of gas solubilities. The first chapter of the book reviews Henr's law, while the second chapter covers the effect of temperature on gas solubility. The third chapter discusses the various gases used by Horiuti, and the following chapters evaluate the data on sulfur dioxide, chlorine data, and solubility data for hydrogen sulfide. Chapter 7 concerns itself with solubility of radon, thoron, and actinon. Chapter 8 tackles the solubilities of diborane and the gaseous hydrides of groups IV, V, and

  10. Chemical composition and organoleptic evaluation of juice from steamed cashew apple blended with orange juice.

    Science.gov (United States)

    Inyang, U E; Abah, U J

    1997-01-01

    Fully riped cashew apples (yellow variety) were steamed for 7 minutes prior to juice extraction. The extracted juice was blended with various proportions of sweet orange juice. Chemical composition and organoleptic evaluation were carried out on both the blended and unblended juices. The ascorbic acid content of unsteamed cashew apple juice was 287 mg/100 ml. Steaming of the cashew apple prior to juice extraction resulted in a decreased (230 mg/100 ml) content of ascorbic acid. It also led to slight decreases in soluble solids and titratable acidity. A comparison of the chemical composition of the two juices showed that the orange juice contained more sugars, titratable acidity and soluble solids but less ascorbic acid than cashew apple juice. Consequently, the soluble solids, titratable acidity, reducing and total sugars of the blends increased with increase in the proportions of orange juice while the content of ascorbic acid was decreasing. In spite of the decrease in ascorbic acid content of the blends, results showed that blended juice would no doubt be a very good source of ascorbic acid. Result of the organoleptic evaluation revealed that a 60% cashew apple and 40% orange juice gave a good quality juice in terms of flavor, after taste and overall acceptability.

  11. Drug-induced low blood sugar

    Science.gov (United States)

    Drug-induced low blood sugar is low blood glucose that results from taking medicine. ... Low blood sugar (hypoglycemia) is common in people with diabetes who are taking insulin or other medicines to control their diabetes. ...

  12. 21 CFR 184.1859 - Invert sugar.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Invert sugar. 184.1859 Section 184.1859 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN... Substances Affirmed as GRAS § 184.1859 Invert sugar. (a) Invert sugar (CAS Reg. No. 8013-17-0) is an aqueous...

  13. Evaluation of thermodynamic properties of solubility of noble gases in nitrogen tetroxide

    International Nuclear Information System (INIS)

    Drugachenok, M.A.; Baklaj, A.A.; Basharina, L.P.

    1986-01-01

    The Henry constants and Gibbs energies of dissolution of noble gases in nitrogen tetroxide have been calculated on the basis of the theory of infinitely dilute solutions. A satisfactory agreement between the calculated and experimental results has been obtained. With the increase of the gas atomic mass, enthalpy of solubility decreases monotonously, so that the process of krypton and xenon slubility in nitrogen tetroxide occurs with heat release. Xenon solubility rises with the increase of temperature. Argon solubility in the condition of operation of the loop plant condenser involves investigation of kinetic behaviour of this process

  14. Hydrotropic solubilization of lipophilic drugs for oral delivery: The effects of urea and nicotinamide on carbamazepine solubility-permeability interplay

    Directory of Open Access Journals (Sweden)

    Avital Beig

    2016-10-01

    Full Text Available Hydrotropy refers to increasing the water solubility of otherwise poorly soluble compound by the presence of small organic molecules. While it can certainly increase the apparent solubility of a lipophilic drug, the effect of hydrotropy on the drugs' permeation through the intestinal membrane has not been studied. The purpose of this work was to investigate the solubility-permeability interplay when using hydrotropic drug solubilization. The concentration-dependent effects of the commonly used hydrotropes urea and nicotinamide, on the solubility and the permeability of the lipophilic antiepileptic drug carbamazepine were studied. Then, the solubility-permeability interplay was mathematically modeled, and was compared to the experimental data. Both hydrotropes allowed significant concentration-dependent carbamazepine solubility increase (up to ~30-fold. A concomitant permeability decrease was evident both in-vitro and in-vivo (~17-fold for nicotinamide and ~9-fold for urea, revealing a solubility-permeability tradeoff when using hydrotropic drug solubilization. A relatively simplified simulation approach based on proportional opposite correlation between the solubility increase and the permeability decrease at a given hydrotrope concentration allowed excellent prediction of the overall solubility-permeability tradeoff. In conclusion, when using hydrotropic drug solubilization it is prudent to not focus solely on solubility, but to account for the permeability as well; achieving optimal solubility-permeability balance may promote the overall goal of the formulation to maximize oral drug exposure.

  15. Possibility as monosaccharide laxative of rare sugar alcohols.

    Science.gov (United States)

    Oosaka, Kazumasa

    2009-05-01

    Allitol, D-talitol and L-iditol are sugar alcohols that are rare in nature. Due to their previous rarity, little is known about the laxative effects of these rare sugar alcohols. Therefore, reliable data on the laxative effect that these sugar alcohols cause in experimental animals could help to evaluate the effectiveness of new monosaccharide laxative drugs. To investigate the laxative effect of rare sugar alcohols, the study was designed to observe the diarrhea that occurred after oral administration of these sugar alcohols in mice. Moreover, to investigate the influence on intestinal function of rare sugar alcohols, the study was designed to examine small intestine transit and the luminal water content. Results indicated that rare sugar alcohols have a laxative effect in mice. Diarrhea started at a dose of 4.95 g/kg of rare sugar alcohols. There was a statistically significant laxative effect for D-talitol and L-iditol at a dose of 9.9 g/kg as compared to vehicle. Moreover, rare sugar alcohols significantly increased the small intestinal transit and the luminal water content of the small intestine and cecum in mice as compared to each vehicle. Overall, L-iditol greatly changes the function of intestine. In conclusion, rare sugar alcohols increase water content in small intestine and accelerate small intestine transit. These results support laxative effect of rare sugar alcohols. Therefore, rare sugar alcohols may be useful as monosaccharide laxatives and may be used to treat constipation.

  16. Reconciliation of opposing views on membrane-sugar interactions

    DEFF Research Database (Denmark)

    Andersen, Heidi D.; Wang, Chunhua; Arleth, Lise

    2011-01-01

    It is well established that small sugars exert different types of stabilization of biomembranes both in vivo and in vitro. However, the essential question of whether sugars are bound to or expelled from membrane surfaces, i.e., the sign and size of the free energy of the interaction, remains...... unresolved, and this prevents a molecular understanding of the stabilizing mechanism. We have used small-angle neutron scattering and thermodynamic measurements to show that sugars may be either bound or expelled depending on the concentration of sugar. At low concentration, small sugars bind quite strongly...... to a lipid bilayer, and the accumulation of sugar at the interface makes the membrane thinner and laterally expanded. Above â¼0.2 M the sugars gradually become expelled from the membrane surface, and this repulsive mode of interaction counteracts membrane thinning. The dual nature of sugar...

  17. Sugars as tobacco ingredient: Effects on mainstream smoke composition.

    Science.gov (United States)

    Talhout, Reinskje; Opperhuizen, Antoon; van Amsterdam, Jan G C

    2006-11-01

    Sugars are natural tobacco components, and are also frequently added to tobacco during the manufacturing process. This review describes the fate of sugars during tobacco smoking, in particular the effect of tobacco sugars on mainstream smoke composition. In natural tobacco, sugars can be present in levels up to 20 wt%. In addition, various sugars are added in tobacco manufacturing in amounts up to 4 wt% per sugar. The added sugars are usually reported to serve as flavour/casing and humectant. However, sugars also promote tobacco smoking, because they generate acids that neutralize the harsh taste and throat impact of tobacco smoke. Moreover, the sweet taste and the agreeable smell of caramelized sugar flavors are appreciated in particular by starting adolescent smokers. Finally, sugars generate acetaldehyde, which has addictive properties and acts synergistically with nicotine in rodents. Apart from these consumption-enhancing pyrolysis products, many toxic (including carcinogenic) smoke compounds are generated from sugars. In particular, sugars increase the level of formaldehyde, acetaldehyde, acetone, acrolein, and 2-furfural in tobacco smoke. It is concluded that sugars in tobacco significantly contribute to the adverse health effects of tobacco smoking.

  18. Monitoring Your Blood Sugar Level

    Science.gov (United States)

    ... and NutritionHealth Insurance: Understanding What It CoversHigh Homocysteine Level: How It Affects Your Blood VesselsUnderstanding Your Medical ... Health Resources Healthcare Management Monitoring Your Blood Sugar Level Monitoring Your Blood Sugar Level Share Print What ...

  19. Polyelectrolyte microcapsules for sustained delivery of water-soluble drugs

    Energy Technology Data Exchange (ETDEWEB)

    Anandhakumar, S.; Debapriya, M. [Department of Materials Engineering, Indian Institute of Science, Bangalore, 560012 (India); Nagaraja, V. [Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012 (India); Raichur, Ashok M., E-mail: amr@materials.iisc.ernet.in [Department of Materials Engineering, Indian Institute of Science, Bangalore, 560012 (India)

    2011-03-12

    Polyelectrolyte capsules composed of weak polyelectrolytes are introduced as a simple and efficient system for spontaneous encapsulation of low molecular weight water-soluble drugs. Polyelectrolyte capsules were prepared by layer-by-layer (LbL) assembling of weak polyelectrolytes, poly(allylamine hydrochloride) (PAH) and poly(methacrylic acid) (PMA) on polystyrene sulfonate (PSS) doped CaCO{sub 3} particles followed by core removal with ethylene-diaminetetraacetic acid (EDTA). The loading process was observed by confocal laser scanning microscopy (CLSM) using tetramethylrhodamineisothiocyanate labeled dextran (TRITC-dextran) as a fluorescent probe. The intensity of fluorescent probe inside the capsule decreased with increase in cross-linking time. Ciprofloxacin hydrochloride (a model water-soluble drug) was spontaneously deposited into PAH/PMA capsules and their morphological changes were investigated by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The quantitative study of drug loading was also elucidated which showed that drug loading increased with initial drug concentration, but decreased with increase in pH. The loaded drug was released in a sustained manner for 6 h, which could be further extended by cross-linking the capsule wall. The released drug showed significant antibacterial activity against E. coli. These findings indicate that such capsules can be potential carriers for water-soluble drugs in sustained/controlled drug delivery applications.

  20. Polyelectrolyte microcapsules for sustained delivery of water-soluble drugs

    International Nuclear Information System (INIS)

    Anandhakumar, S.; Debapriya, M.; Nagaraja, V.; Raichur, Ashok M.

    2011-01-01

    Polyelectrolyte capsules composed of weak polyelectrolytes are introduced as a simple and efficient system for spontaneous encapsulation of low molecular weight water-soluble drugs. Polyelectrolyte capsules were prepared by layer-by-layer (LbL) assembling of weak polyelectrolytes, poly(allylamine hydrochloride) (PAH) and poly(methacrylic acid) (PMA) on polystyrene sulfonate (PSS) doped CaCO 3 particles followed by core removal with ethylene-diaminetetraacetic acid (EDTA). The loading process was observed by confocal laser scanning microscopy (CLSM) using tetramethylrhodamineisothiocyanate labeled dextran (TRITC-dextran) as a fluorescent probe. The intensity of fluorescent probe inside the capsule decreased with increase in cross-linking time. Ciprofloxacin hydrochloride (a model water-soluble drug) was spontaneously deposited into PAH/PMA capsules and their morphological changes were investigated by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The quantitative study of drug loading was also elucidated which showed that drug loading increased with initial drug concentration, but decreased with increase in pH. The loaded drug was released in a sustained manner for 6 h, which could be further extended by cross-linking the capsule wall. The released drug showed significant antibacterial activity against E. coli. These findings indicate that such capsules can be potential carriers for water-soluble drugs in sustained/controlled drug delivery applications.

  1. Mitochondrial Dysfunction Plus High-Sugar Diet Provokes a Metabolic Crisis That Inhibits Growth

    Science.gov (United States)

    Kemppainen, Esko; George, Jack; Garipler, Görkem; Tuomela, Tea; Kiviranta, Essi; Soga, Tomoyoshi; Dunn, Cory D.; Jacobs, Howard T.

    2016-01-01

    The Drosophila mutant tko25t exhibits a deficiency of mitochondrial protein synthesis, leading to a global insufficiency of respiration and oxidative phosphorylation. This entrains an organismal phenotype of developmental delay and sensitivity to seizures induced by mechanical stress. We found that the mutant phenotype is exacerbated in a dose-dependent fashion by high dietary sugar levels. tko25t larvae were found to exhibit severe metabolic abnormalities that were further accentuated by high-sugar diet. These include elevated pyruvate and lactate, decreased ATP and NADPH. Dietary pyruvate or lactate supplementation phenocopied the effects of high sugar. Based on tissue-specific rescue, the crucial tissue in which this metabolic crisis initiates is the gut. It is accompanied by down-regulation of the apparatus of cytosolic protein synthesis and secretion at both the RNA and post-translational levels, including a novel regulation of S6 kinase at the protein level. PMID:26812173

  2. Mitochondrial Dysfunction Plus High-Sugar Diet Provokes a Metabolic Crisis That Inhibits Growth.

    Science.gov (United States)

    Kemppainen, Esko; George, Jack; Garipler, Görkem; Tuomela, Tea; Kiviranta, Essi; Soga, Tomoyoshi; Dunn, Cory D; Jacobs, Howard T

    2016-01-01

    The Drosophila mutant tko25t exhibits a deficiency of mitochondrial protein synthesis, leading to a global insufficiency of respiration and oxidative phosphorylation. This entrains an organismal phenotype of developmental delay and sensitivity to seizures induced by mechanical stress. We found that the mutant phenotype is exacerbated in a dose-dependent fashion by high dietary sugar levels. tko25t larvae were found to exhibit severe metabolic abnormalities that were further accentuated by high-sugar diet. These include elevated pyruvate and lactate, decreased ATP and NADPH. Dietary pyruvate or lactate supplementation phenocopied the effects of high sugar. Based on tissue-specific rescue, the crucial tissue in which this metabolic crisis initiates is the gut. It is accompanied by down-regulation of the apparatus of cytosolic protein synthesis and secretion at both the RNA and post-translational levels, including a novel regulation of S6 kinase at the protein level.

  3. Response of a two-year sugar beet-sweet sorghum rotation to an agronomic management approach diversified by soil tillage and nitrogen fertilisation

    Directory of Open Access Journals (Sweden)

    A. Domenico Palumbo

    2014-08-01

    Full Text Available Conservative agriculture and nitrogen fertilisation have been evaluated for the purpose of assessing their impact on the sustainability of a cropping system based on a two-year rotation with two crops considered for the bio-ethanol supply chain: sugar beet (Beta vulgaris L. subsp. vulgaris and sweet sorghum (Sorghum bicolor L. Moench. The experimental activity started in 2009 in Foggia (Apulia, southern Italy. We discuss the results obtained in the 2010-2011 period. Soil minimum tillage (MT vs no tillage (NT combined with two doses of nitrogen fertilisation (75 and 150 kg ha–1 of mineral nitrogen as ammonium nitrate were compared. The experimental system, which is still operational (soil tillage plus nitrogen fertilisation, was arranged with a split-plot design with three replicates. Treatments were applied on the same plots every year with both crops present at the same time. At the first harvest in 2010, no difference was observed. As to the second year, the comparison between NT vs MT treatments showed that sugar beet had lower total yield (35 vs 42 t ha–1, dry biomass (10 vs 14 t ha–1, and sucrose yield (6.7 vs 8.2 t ha–1. Total soluble solids, on average 19%, were not influenced by the experimental treatments. Nitrogen (N control was less productive than the fertilised treatments (average between N75 and N150 in terms of total fresh root yield (32 vs 42 t ha–1, dry biomass (10 vs 14 t ha–1, and sucrose yield (6.0 vs 8.1 t ha–1. As with sugar beet, during the second year, also sweet sorghum sown in NT vs MT plots had a reduced yield, although the difference was more marked for fresh biomass (–35% than for dry biomass (–20%. No interaction in terms of soil tillage nitrogen fertilisation occurred. In summary, in the first two-year period (2010-2011 of the experimental trial, no tillage soil management showed decreased yields of both crops. Sugar beet displayed a higher sensitivity to the lack of nitrogen supply than sweet

  4. An econometrics method to estimate demand of sugar

    Directory of Open Access Journals (Sweden)

    Negar Seyed Soleimany

    2012-01-01

    Full Text Available Sugar is one of the strategic goods in the basket of households in each country and it plays an important role in supplying the required energy. On the other hand, it is one of the goods, which Iranian government is about to change its subsidy strategies. To design useful sugar subsidy strategies, it is necessary to know sugar position in the basket of households and be familiar with households' sugar demand or consumption behavior. This research estimates sugar demand for Iranian households by using time series of 1984-2008, which is taken from central bank of Iran. In this paper, first independent and dependent variables of household sugar demand model are chosen based on the literature review and theory of demand. Then, sugar demand is estimated by OLS technique and linear regression. The preliminary statistical observations such as Durbin-Watson, F statistic and R2 indicate that the regression is admissible. The results seem plausible and consistent with theory and show that sugar demand in Iranian households is associated with household expenditure, relative sugar price, family size and indicate that demand of sugar is affected during the war time. The results also show the income elasticity is 0.8 and price elasticity is -0.2 which means sugar is essential good for Iranian households and is inelastic to price.

  5. Combination of sugar analysis and stable isotope ratio mass spectrometry to detect the use of artificial sugars in royal jelly production.

    Science.gov (United States)

    Wytrychowski, Marine; Daniele, Gaëlle; Casabianca, Hervé

    2012-05-01

    The effects of feeding bees artificial sugars and/or proteins on the sugar compositions and (13)C isotopic measurements of royal jellies (RJs) were evaluated. The sugars fed to the bees were two C4 sugars (cane sugar and maize hydrolysate), two C3 sugars (sugar beet, cereal starch hydrolysate), and honey. The proteins fed to them were pollen, soybean, and yeast powder proteins. To evaluate the influence of the sugar and/or protein feeding over time, samples were collected during six consecutive harvests. (13)C isotopic ratio measurements of natural RJs gave values of around -25 ‰, which were also seen for RJs obtained when the bees were fed honey or C3 sugars. However, the RJs obtained when the bees were fed cane sugar or corn hydrolysate (regardless of whether they were also fed proteins) gave values of up to -17 ‰. Sugar content analysis revealed that the composition of maltose, maltotriose, sucrose, and erlose varied significantly over time in accordance with the composition of the syrup fed to the bees. When corn and cereal starch hydrolysates were fed to the bees, the maltose and maltotriose contents of the RJs increased up to 5.0 and 1.3 %, respectively, compared to the levels seen in authentic samples (i.e., samples obtained when the bees were fed natural food: honey and pollen) that were inferior to 0.2% and not detected, respectively. The sucrose and erlose contents of natural RJs were around 0.2 %, whereas those in RJs obtained when the bees were fed cane or beet sugar were as much as 4.0 and 1.3 %, respectively. The combination of sugar analysis and (13)C isotopic ratio measurements represents a very efficient analytical methodology for detecting (from early harvests onward) the use of C4 and C3 artificial sugars in the production of RJ.

  6. Silencing a sugar transporter gene reduces growth and fecundity in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae).

    Science.gov (United States)

    Ge, Lin-Quan; Jiang, Yi-Ping; Xia, Ting; Song, Qi-Sheng; Stanley, David; Kuai, Peng; Lu, Xiu-Li; Yang, Guo-Qing; Wu, Jin-Cai

    2015-07-17

    The brown planthopper (BPH), Nilaparvata lugens, sugar transporter gene 6 (Nlst6) is a facilitative glucose/fructose transporter (often called a passive carrier) expressed in midgut that mediates sugar transport from the midgut lumen to hemolymph. The influence of down regulating expression of sugar transporter genes on insect growth, development, and fecundity is unknown. Nonetheless, it is reasonable to suspect that transporter-mediated uptake of dietary sugar is essential to the biology of phloem-feeding insects. Based on this reasoning, we posed the hypothesis that silencing, or reducing expression, of a BPH sugar transporter gene would be deleterious to the insects. To test our hypothesis, we examined the effects of Nlst6 knockdown on BPH biology. Reducing expression of Nlst6 led to profound effects on BPHs. It significantly prolonged the pre-oviposition period, shortened the oviposition period, decreased the number of eggs deposited and reduced body weight, compared to controls. Nlst6 knockdown also significantly decreased fat body and ovarian (particularly vitellogenin) protein content as well as vitellogenin gene expression. Experimental BPHs accumulated less fat body glucose compared to controls. We infer that Nlst6 acts in BPH growth and fecundity, and has potential as a novel target gene for control of phloem-feeding pest insects.

  7. Fermentation of sugar-beet molasses

    Energy Technology Data Exchange (ETDEWEB)

    Malchenko, A L; Krishtul, F B

    1956-08-25

    Sugar-beet molasses is fermented with yeast separated from the mash, sterilized, and reactivated. To reduce sugar losses and hasten fermentation, the yeast is removed from the mash as the cells fall to the bottom during the fermentation process.

  8. [The energy density and the nutritional quality of diet depending on their sugar content].

    Science.gov (United States)

    Martínez Álvarez, Jesús Román

    2013-07-01

    Sugar content in foods cannot be distinguished from added sugar, although it is true that this added sugar brings mainly energy and no other essential nutrients. On the other hand, in the context of diet, sugar helps make it more varied and palatable allowing including foods that may otherwise not be would consume, thus indirectly contributing to the intake of other nutrients. Having interest in knowing the possible relationship between a high intake of sugars and the decrease in micronutrients intake, we noted that the nutrient density of the diet might be influenced by factors such as the high presence of sugar added to food. It seems that this nutritional dilution produced by adding sugar to food is, in general, not very significant and, often, offset by the fortification in micronutrients that we usually can find in many sugary products. After a detailed analysis of the published studies on the subject, it has been found that there is no a clear evidence of the hypothetical micronutrient dilution that would occur by adding sugar to the diet. On the other hand, given that the addition of sugar to the diet doesn't seem to report any remarkable advantages from the point of view of the intake of micronutrients; It seems reasonable to promote a moderate consumption of foods and sugary drinks, so in that way, they become an important extra energy source. It should also be borne in mind that the addition of sugar to the diet does not seem remarkable report any advantage in terms of intake of micronutrients. For this reason, it seems logical that the consumption of sugary food and sweet drinks will be moderate given the ease of consumption and the likelihood of their becoming a major source of energy. Finally, it is concluded on the need for further research on the mechanisms underlying that, up to now showed no, possible displacement of micro-nutrients and other food components of the diet that could occur in cases of consumption of foods with a high sugar content, as

  9. Effect of commonly consumed sugar containing and sugar free fruit drinks on the hydrogen ion modulation of human dental plaque

    Directory of Open Access Journals (Sweden)

    Nanika Mahajan

    2014-01-01

    Full Text Available Background: With the increased awareness of healthy diet among the population, the intake of fruit juices as health drinks has been increased. This study has been designed to check the potential cariogenicity of fruit drinks frequently consumed by infants and young children. Aim: To compare the acidogenic potential of sugar free fruit juices with fruit juices containing sugar by evaluating the plaque pH changes, following consumption of the above drinks. Design: The study was carried out on 10 children in the age group of 8-15 years. The four fruit juices used were 1 orange juice with added sugar 2 orange juice with no added sugar 3 apple juice with added sugar 4 apple juice with no added sugar. Sucrose rinse of 10% was used as control group. The endogenous pH of the fruit juices and control was assessed using digital pH meter. The plaque pH was assessed at the baseline and after the consumption of the drinks at 5, 10, 20, 30, 40, and 60 minutes time interval using the plaque-harvesting technique. The obtained results were compiled and subjected to statistical analysis using paired t-test. Result: All the fruit juices showed drop in plaque pH. A drop in pH was also observed in the juices despite of no added sugar content. Conclusion: The fruit juices labeled with "no added sugar" or "free from added sugar", contained substantial quantities of sugar and are equally cariogenic as are fruit drinks with added sugar.

  10. COMPARISON OF PRETREATMENT STRATEGIES FOR CONVERSION OF COCONUT HUSK FIBER TO FERMENTABLE SUGARS

    Directory of Open Access Journals (Sweden)

    Teck Y. Ding,

    2012-02-01

    Full Text Available In the present study, coconut husk was employed as biomass feedstock for production of bioethanol, due to its abundance in Malaysia. Due to the complex structures of coconut husk, a pretreatment process is crucial in extracting fermentable sugars from the embedded cellulose matrix for subsequent ethanol fermentation process. The ground coconut husk was subjected to three different pretreatment processes inclusive of thermal, chemical, and microwave-assisted-alkaline techniques, prior to enzymatic hydrolysis and fermentation process. The composition profile of coconut husk was significantly altered upon the microwave-assisted-alkaline treatment as compared to the untreated sample, with the cellulose content increasing from 18-21% to 38-39% while lignin content decreased from 46-53% to 31-33%. Among the pretreatment methods applied, enzymatic hydrolysis of coconut husk pretreated by microwave-assisted-alkaline method recorded the highest yield of fermentable sugars, 0.279 g sugar/g substrate. SEM imaging showed the obvious and significant disruption of coconut husks’ structure after microwave-assisted-alkaline pretreatment. In conclusion, by employing suitable pretreatment technique in treating the lignocellulosic materials of coconut husk, the extracted fermentable sugar is a potential substrate for bioethanol production.

  11. Tagging of resistance gene(s) to rhizomania disease in sugar beet ...

    African Journals Online (AJOL)

    The rhizomania disease is one of the most important diseases in Iran and some other parts of the world which potentially could play a role in decreasing sugar yield in fields. One approach to combat with this disease is the use of resistance varieties. This varieties have been identified which are having resistance genes to ...

  12. SILAGE CANE SUGAR ADDED WITH DRIED BREWER

    Directory of Open Access Journals (Sweden)

    W. J. R. Castro

    2015-02-01

    Full Text Available The objective of this experiment was to evaluate the fermentative parameters and chemical composition of silage cane sugar added with residue dried brewery. The experimental design was completely randomized with four treatments and four replications: 100% cane sugar; 90% of cane sugar + 10% residue dried brewer; 80% of cane sugar + 20% residue dried brewer and 70% cane sugar + 30% dried brewer based on natural matter, composed silages. The sugar cane was chopped in a stationary machine with forage particle size of approximately 2 cm, and homogenized manually with the additives. For storage chopped fresh weight were used in experimental silos capacity of about 4 liters. The results showed that the contents of dry matter and crude protein showed positive linear (P0.05 with mean value of 3.81, while for ether extract and ash results were positive linear (P0.05 for N ammonia presented average value of 4.18. It is concluded that the addition of brewer dehydrated improves the fermentation process of silage cane sugar, in addition to improving their nutritional characteristics.

  13. Supply optimization for the production of raw sugar

    DEFF Research Database (Denmark)

    Grunow, Martin; Günther, H.O.; Westinner, R.

    2007-01-01

    Based on a case study from Venezuela, the production of raw sugar is investigated. Ideally, sugar mills operate at a constant production rate. However, safety stocks of the raw material cannot be maintained as sugar cane quality deteriorates very rapidly. Sugar cane is therefore continuously...

  14. A study of transformation water - soluble forms of hevy metals at waste incenerator for detoxicationof ash.

    Directory of Open Access Journals (Sweden)

    Bilets'ka V. А.

    2011-11-01

    Full Text Available The complex research processes of transformation of soluble forms of heavy metals in sediment interaction with ash. Proved that the adsorption processes of immobilization lead to a significant decrease of soluble forms of heavy metals in the waste.

  15. Allelopathic effects of barley straw on germination and seedling growth of corn, sugar beet and sunflower

    Directory of Open Access Journals (Sweden)

    mohamad taghi naseri poor yazdi

    2009-06-01

    Full Text Available Allelopathic effects of barley straw and root on germination and growth of maize, sugar beet, and sunflower were investigated under glasshouse and laboratory experiments in Faculty of Agriculture, Ferdowsi University of Mashhad in 2006. The glasshouse experiment was designed based on randomized complete block design with three replications, treatments included: 0, 200, 400, 600 g/m² of grounded barley straw and also 0 and 50 g/m2 barley root. A laboratory experiment was carried out in order to study the effect of different concentrations of barley water extracts on germination and seedling characteristics of corn, sugar beet and sunflower. Treatments in laboratory trial included 0, 33, 50 and 100 percent of barley extracts. Results showed that leaf area of corn was significantly affected by barley straw treatments. Shoot dry matter and seed weight per plant in corn , leaf and tuber weight in sugar beet and leaf , stem weights , plant per plant in corn , leaf and tuber weight in sugar beet and leaf, stem weights, plant height, head diameter, head weight and seed weight in sunflower were significantly higher in treatment of 50g/m² barley roots. Crop seed germination decreased with increasing the amount of barley straw. The best germination response to barley extract was observed in corn. Maize radicle weight was significantly decreased with increasing concentration of barley water extract.

  16. Manage your blood sugar (image)

    Science.gov (United States)

    Checking your blood sugar levels often and writing down the results will tell you how well you are managing your diabetes so you ... possible. The best times to check your blood sugar are before meals and at bedtime. Your blood ...

  17. Are restrictive guidelines for added sugars science based?

    Science.gov (United States)

    Erickson, Jennifer; Slavin, Joanne

    2015-12-12

    Added sugar regulations and recommendations have been proposed by policy makers around the world. With no universal definition, limited access to added sugar values in food products and no analytical difference from intrinsic sugars, added sugar recommendations present a unique challenge. Average added sugar intake by American adults is approximately 13% of total energy intake, and recommendations have been made as low 5% of total energy intake. In addition to public health recommendations, the Food and Drug Administration has proposed the inclusion of added sugar data to the Nutrition and Supplemental Facts Panel. The adoption of such regulations would have implications for both consumers as well as the food industry. There are certainly advantages to including added sugar data to the Nutrition Facts Panel; however, consumer research does not consistently show the addition of this information to improve consumer knowledge. With excess calorie consumption resulting in weight gain and increased risk of obesity and obesity related co-morbidities, added sugar consumption should be minimized. However, there is currently no evidence stating that added sugar is more harmful than excess calories from any other food source. The addition of restrictive added sugar recommendations may not be the most effective intervention in the treatment and prevention of obesity and other health concerns.

  18. Synthesis gas solubility in Fischer-Tropsch slurry: Final report

    Energy Technology Data Exchange (ETDEWEB)

    Chao, K.C.; Lin, H.M.

    1988-01-01

    The objective is to investigate the phase equilibrium behavior of synthesis gases and products in a Fischer-Tropsch slurry reactor. A semi-flow apparatus has been designed and constructed for this purpose. Measurements have been made for hydrogen, cabon monoxide, methane, ethane, ethylene, and carbon dioxide in a heavy n-paraffin at temperatures from 100 to 300)degree)C and pressures 10 to 50 atm. Three n-paraffin waxes: n-eicosane (n-C/sub 20/), n-octacosane )n-C/sub 28/), and n-hexatriacontane (n-C/sub 36/), were studied to model the industrial wax. Solubility of synthesis gas mixtures of H/sub 2/ and CO in n-C/sub 28/ was also determined at two temperatures (200 and 300)degree)C) for each of three gas compositions (40.01, 50.01, and 66.64 mol%) of hydrogen). Measurements were extended to investigate the gas solubility in two industrial Fischer-Tropsch waxes: Mobilwax and SASOL wax. Observed solubility increases in the order: H/sub 2/, CO, CH/sub 4/, CO/sub 2/, C/sub 2/H/sub 4/, C/sub 2/H/sub 6/, at a given temperature pressure, and in the same solvent. Solubility increases with increasing pressure for all the gases. Lighter gases H/sub 2/ and CO show increased solubility with increasing temperature, while the heavier gases CO/sub 2/, ethane, and ethylene show decreased solubility with increasing temperature. The solubility of methane, the intermediate gas, changes little with temperature, and shows a shallow minimum at about 200)degrees)C or somewhat above. Henry's constant and partial molal volume of the gas solute at infinite dilution are determinedfrom the gas solubility data. A correlation is developed from the experimental data in the form on an equation of state. A computer program has been prepared to implement the correlation. 19 refs., 66 figs., 39 tabs.

  19. The determination of solubility and diffusion coefficient for solids in liquids by an inverse measurement technique using cylinders of amorphous glucose as a model compound

    International Nuclear Information System (INIS)

    Hu, Chengyao; Huang, Pei

    2011-01-01

    The importance of sugar and sugar-containing materials is well recognized nowadays, owing to their application in industrial processes, particularly in the food, pharmaceutical and cosmetic industries. Because of the large numbers of those compounds involved and the relatively small number of solubility and/or diffusion coefficient data for each compound available, it is highly desirable to measure the solubility and/or diffusion coefficient as efficiently as possible and to be able to improve the accuracy of the methods used. In this work, a new technique was developed for the measurement of the diffusion coefficient of a stationary solid solute in a stagnant solvent which simultaneously measures solubility based on an inverse measurement problem algorithm with the real-time dissolved amount profile as a function of time. This study differs from established techniques in both the experimental method and the data analysis. The experimental method was developed in which the dissolved amount of solid solute in quiescent solvent was investigated using a continuous weighing technique. In the data analysis, the hybrid genetic algorithm is used to minimize an objective function containing a calculated and a measured dissolved amount with time. This is measured on a cylindrical sample of amorphous glucose in methanol or ethanol. The calculated dissolved amount, that is a function of the unknown physical properties of the solid solute in the solvent, is calculated by the solution of the two-dimensional nonlinear inverse natural convection problem. The estimated values of the solubility of amorphous glucose in methanol and ethanol at 293 K were respectively 32.1 g/100 g methanol and 1.48 g/100 g ethanol, in agreement with the literature values, and support the validity of the simultaneously measured diffusion coefficient. These results show the efficiency and the stability of the developed technique to simultaneously estimate the solubility and diffusion coefficient. Also

  20. Residues in the H+ Translocation Site Define the pKa for Sugar Binding to LacY†

    Science.gov (United States)

    Smirnova, Irina; Kasho, Vladimir; Sugihara, Junichi; Choe, Jun-Yong; Kaback, H. Ronald

    2009-01-01

    A remarkably high pKa of approximately 10.5 has been determined for sugar-binding affinity to the lactose permease of Escherichia coli (LacY), indicating that, under physiological conditions, substrate binds to fully protonated LacY. We have now systematically tested site-directed replacements for the residues involved in sugar binding, as well as H+ translocation and coupling, in order to determine which residues may be responsible for this alkaline pKa. Mutations in the sugar-binding site (Glu126, Trp151, Glu269) markedly decrease affinity for sugar but do not alter the pKa for binding. In contrast, replacements for residues involved in H+ translocation (Arg302, Tyr236, His322, Asp240, Glu325, Lys319) exhibit pKa values for sugar binding that are either shifted toward neutral pH or independent of pH. Values for the apparent dissociation constant for sugar binding (Kdapp) increase greatly for all mutants except neutral replacements for Glu325 or Lys319, which are characterized by remarkably high affinity sugar binding (i.e., low Kdapp) from pH 5.5 to pH 11. The pH dependence of the on- and off-rate constants for sugar binding measured directly by stopped-flow fluorometry implicates koff as a major factor for the affinity change at alkaline pH and confirms the effects of pH on Kdapp inferred from steady-state fluorometry. These results indicate that the high pKa for sugar binding by wild-type LacY cannot be ascribed to any single amino acid residue but appears to reside within a complex of residues involved in H+ translocation. There is structural evidence for water bound in this complex, and the water could be the site of protonation responsible for the pH dependence of sugar binding. PMID:19689129

  1. Evaluation of Physical-Chemical Indexes, Sugars, Pigments and Phenolic Compounds of Fruits from Three Apple Varieties at the End of Storage Period

    Directory of Open Access Journals (Sweden)

    Andruta Elena Muresan

    2014-05-01

    Full Text Available Apples are the most cultivated and consumed fruits in the world. They not only taste great, but there are also rich sources of monosaccharides, pigments, fibers, functional compounds such as polyphenols which are well-known for their antioxidant action. Due to the high level of apples consumption, it is important to monitor and know the detailed chemical composition of this fruits on the market shelf. The aim of this paper was to study the detailed chemical composition of apples from three varieties. Samples from three varieties (Ionathan, Golden Delicious and Starkrimson were taken from the Romanian market. Individual sugars composition was performed by HPLC, total polyphenols content by Folin Ciocalteu method, antioxidant capacity by using the DPPH test, while pigments were analysed by spectrophotometric specific methods and the total starch content measured by a polarimetric method. Water content, acidity, total soluble solids and pH were also monitored through specific methods. There were found differences between varieties particularly in relation to the polyphenols content, carotenoids and chlorophyll. Regarding the individual sugars composition, fructose and glucose were predominant followed by sucrose for all samples. Values of starch, moisture, acidity, total soluble solids and the pH were according to other apple varieties found in literature. These results provide important information regarding the chemical composition of apple varieties from Romanian market, for both human direct consumption and industrial processing. 

  2. 21 CFR 184.1857 - Corn sugar.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Corn sugar. 184.1857 Section 184.1857 Food and... Substances Affirmed as GRAS § 184.1857 Corn sugar. (a) Corn sugar (C6H12O6, CAS Reg. No. 50-99-7), commonly... monohydrate form and is produced by the complete hydrolysis of corn starch with safe and suitable acids or...

  3. Cross-sectional survey of the amount of free sugars and calories in carbonated sugar-sweetened beverages on sale in the UK

    Science.gov (United States)

    Hashem, Kawther M; He, Feng J; Jenner, Katharine H; MacGregor, Graham A

    2016-01-01

    Objectives To investigate the free sugars and calorie content of carbonated sugar-sweetened beverages (CSSB) available in the main UK supermarkets. Study design We carried out a cross-sectional survey in 2014 of 169 CSSB. Methods The free sugars (sugars g/100 mL) and calorie (kcal/100 mL) were collected from product packaging and nutrient information panels of CSSB available in 9 main UK supermarkets. Results The average free sugars content in CSSB was 30.1±10.7 g/330 mL, and 91% of CSSB would receive a ‘red’ (high) label for sugars per serving. There was a large variation in sugars content between different flavours of CSSB and within the same type of flavour ranging from 3.3 to 52.8 g/330 mL. On average, ginger beer (38.5±9.9 g/330 mL) contained the highest amounts of sugars and ginger ale (22.9±7.7 g/330 mL) contained the lowest. Cola flavour is the most popular flavour in the UK with an average free sugars content of 35.0±1.1 g/330 mL. On average, the supermarket own brand contained lower levels of sugars than branded products (27.9±10.6 vs 31.6±10.6 g/330 mL, p=0.02). The average calorie content in CSSB was 126.1±43.5 kcal/330 mL. Cola flavour had a calorie content of 143.5±5.2 kcal/330 mL. Among the 169 products surveyed, 55% exceeded the maximum daily recommendation for free sugars intake (30 g) per 330 mL. Conclusions Free sugars content of CSSB in the UK is high and is a major contributor to free sugars intake. There is a wide variation in the sugars content of CSSB and even within the same flavour of CSSB. These findings demonstrate that the amount of free sugars added to CSSB can be reduced without technical issues, and there is an urgent need to set incremental free sugars reduction targets. A reduction in sugars content and overall CSSB consumption will be very beneficial in reducing obesity, type 2 diabetes and dental caries. PMID:28186923

  4. Effect of accessions on the chemical quality of fresh pumpkin ...

    African Journals Online (AJOL)

    Twenty (20) fresh pumpkin fruits were assessed for dry matter, total soluble solid, total sugar, reducing sugar, titratable acidity, pH, ascorbic acid and sugar-to-acid ratio. Statistically significant (P < 0.01) differences were found for dry matter, total soluble solid, total sugar, reducing sugar, titratable acidity, ascorbic acid, pH ...

  5. THE FACTORS FORMING QUALITY OF GRANULATED SUGAR

    Directory of Open Access Journals (Sweden)

    N. G. Kulneva

    2015-01-01

    Full Text Available Sugar, with good taste and high caloric, is one of the most popular human food. Consumers sugar must be sure that the sugar under normal conditions of use is of high quality and is not harmful to the health of the product. One reason for the decline in the quality of sugar is bacterial contamination. This is because the sugar industry products are good targets for the development of different groups of microorganisms, e.g., Bacillus subtilis, Clostridium perfringes, Leuconostoc dextranicum, Torula alba, Pseudomonas fluorescens, Sarcina lutea and others. These organisms are affected with beets, and then with sugar beet chips and diffusion juice fall into the processing line of sugar production. Their number in the diffusion juice varies and depends on many facto rs such as the quality of raw materials, the quality of cleaning beet root colonization of transporter-washing and the supply of water to the diffusion process, the temperature of the diffusion and others. In the diffusion unit has the most favorable conditions for the development of micro-organisms. Some of them, especially resistant bacteria and thermophilic bacteria or their spores, forming a capsule which protects against external influences occur in the final product sugar. When injected into the fresh crop of product (juice, syrup, they begin to multiply rapidly, causing difficulties in the process. The higher seeding beet microorganisms, the more they decompose and emit sucrose metabolism byproducts. To reduce the negative impact of microbiological and reduce losses from decomposition of sucrose conducted research on the possibility of using chlorine-containing substances in the sugar industry. It was established experimentally that the investigated chlorinated drug has bacteriostatic action and can be recommended for use in sugar beet production.

  6. Taxation of unprocessed sugar or sugar-added foods for reducing their consumption and preventing obesity or other adverse health outcomes: Protocol

    OpenAIRE

    Pfinder, Manuela; Katikireddi, Srinivasa V.; Pega, Frank; Gartlehner, Gerald; Fenton, Candida; Griebler, Ursula; Sommer, Isolde; Heise, Thomas L.; Lhachimi, Stefan K.

    2016-01-01

    To assess the effects of taxation of unprocessed sugar or sugar-added foods in the general population on the:\\ud \\ud consumption of unprocessed sugar or sugar-added foods;\\ud prevalence and incidence of overweight and obesity; and\\ud prevalence and incidence of diet-related health conditions.

  7. Effects of zinc toxicity on sugar beet (Beta vulgaris L.) plants grown in hydroponics.

    Science.gov (United States)

    Sagardoy, R; Morales, F; López-Millán, A-F; Abadía, A; Abadía, J

    2009-05-01

    The effects of high Zn concentration were investigated in sugar beet (Beta vulgaris L.) plants grown in a controlled environment in hydroponics. High concentrations of Zn sulphate in the nutrient solution (50, 100 and 300 microm) decreased root and shoot fresh and dry mass, and increased root/shoot ratios, when compared to control conditions (1.2 microm Zn). Plants grown with excess Zn had inward-rolled leaf edges and a damaged and brownish root system, with short lateral roots. High Zn decreased N, Mg, K and Mn concentrations in all plant parts, whereas P and Ca concentrations increased, but only in shoots. Leaves of plants treated with 50 and 100 microm Zn developed symptoms of Fe deficiency, including decreases in Fe, chlorophyll and carotenoid concentrations, increases in carotenoid/chlorophyll and chlorophyll a/b ratios and de-epoxidation of violaxanthin cycle pigments. Plants grown with 300 microm Zn had decreased photosystem II efficiency and further growth decreases but did not have leaf Fe deficiency symptoms. Leaf Zn concentrations of plants grown with excess Zn were high but fairly constant (230-260 microg.g(-1) dry weight), whereas total Zn uptake per plant decreased markedly with high Zn supply. These data indicate that sugar beet could be a good model to investigate Zn homeostasis mechanisms in plants, but is not an efficient species for Zn phytoremediation.

  8. Soluble polymer conjugates for drug delivery.

    Science.gov (United States)

    Minko, Tamara

    2005-01-01

    The use of water-soluble polymeric conjugates as drug carriers offers several possible advantages. These advantages include: (1) improved drug pharmacokinetics; (2) decreased toxicity to healthy organs; (3) possible facilitation of accumulation and preferential uptake by targeted cells; (4) programmed profile of drug release. In this review, we will consider the main types of useful polymeric conjugates and their role and effectiveness as carriers in drug delivery systems.: © 2005 Elsevier Ltd . All rights reserved.

  9. Estimating Free and Added Sugar Intakes in New Zealand

    Directory of Open Access Journals (Sweden)

    Rachael Kibblewhite

    2017-11-01

    Full Text Available The reduction of free or added sugar intake (sugars added to food and drinks as a sweetener is almost universally recommended to reduce the risk of obesity-related diseases and dental caries. The World Health Organisation recommends intakes of free sugars of less than 10% of energy intake. However, estimating and monitoring intakes at the population level is challenging because free sugars cannot be analytically distinguished from naturally occurring sugars and most national food composition databases do not include data on free or added sugars. We developed free and added sugar estimates for the New Zealand (NZ food composition database (FOODfiles 2010 by adapting a method developed for Australia. We reanalyzed the 24 h recall dietary data collected for 4721 adults aged 15 years and over participating in the nationally representative 2008/09 New Zealand Adult Nutrition Survey to estimate free and added sugar intakes. The median estimated intake of free and added sugars was 57 and 49 g/day respectively and 42% of adults consumed less than 10% of their energy intake from free sugars. This approach provides more direct estimates of the free and added sugar contents of New Zealand foods than previously available and will enable monitoring of adherence to free sugar intake guidelines in future.

  10. Estimating Free and Added Sugar Intakes in New Zealand.

    Science.gov (United States)

    Kibblewhite, Rachael; Nettleton, Alice; McLean, Rachael; Haszard, Jillian; Fleming, Elizabeth; Kruimer, Devonia; Te Morenga, Lisa

    2017-11-27

    The reduction of free or added sugar intake (sugars added to food and drinks as a sweetener) is almost universally recommended to reduce the risk of obesity-related diseases and dental caries. The World Health Organisation recommends intakes of free sugars of less than 10% of energy intake. However, estimating and monitoring intakes at the population level is challenging because free sugars cannot be analytically distinguished from naturally occurring sugars and most national food composition databases do not include data on free or added sugars. We developed free and added sugar estimates for the New Zealand (NZ) food composition database (FOODfiles 2010) by adapting a method developed for Australia. We reanalyzed the 24 h recall dietary data collected for 4721 adults aged 15 years and over participating in the nationally representative 2008/09 New Zealand Adult Nutrition Survey to estimate free and added sugar intakes. The median estimated intake of free and added sugars was 57 and 49 g/day respectively and 42% of adults consumed less than 10% of their energy intake from free sugars. This approach provides more direct estimates of the free and added sugar contents of New Zealand foods than previously available and will enable monitoring of adherence to free sugar intake guidelines in future.

  11. Estimating Free and Added Sugar Intakes in New Zealand

    Science.gov (United States)

    Kibblewhite, Rachael; Nettleton, Alice; McLean, Rachael; Haszard, Jillian; Fleming, Elizabeth; Kruimer, Devonia

    2017-01-01

    The reduction of free or added sugar intake (sugars added to food and drinks as a sweetener) is almost universally recommended to reduce the risk of obesity-related diseases and dental caries. The World Health Organisation recommends intakes of free sugars of less than 10% of energy intake. However, estimating and monitoring intakes at the population level is challenging because free sugars cannot be analytically distinguished from naturally occurring sugars and most national food composition databases do not include data on free or added sugars. We developed free and added sugar estimates for the New Zealand (NZ) food composition database (FOODfiles 2010) by adapting a method developed for Australia. We reanalyzed the 24 h recall dietary data collected for 4721 adults aged 15 years and over participating in the nationally representative 2008/09 New Zealand Adult Nutrition Survey to estimate free and added sugar intakes. The median estimated intake of free and added sugars was 57 and 49 g/day respectively and 42% of adults consumed less than 10% of their energy intake from free sugars. This approach provides more direct estimates of the free and added sugar contents of New Zealand foods than previously available and will enable monitoring of adherence to free sugar intake guidelines in future. PMID:29186927

  12. Fatty acid synthase cooperates with glyoxalase 1 to protect against sugar toxicity.

    Directory of Open Access Journals (Sweden)

    Damien Garrido

    2015-02-01

    Full Text Available Fatty acid (FA metabolism is deregulated in several human diseases including metabolic syndrome, type 2 diabetes and cancers. Therefore, FA-metabolic enzymes are potential targets for drug therapy, although the consequence of these treatments must be precisely evaluated at the organismal and cellular levels. In healthy organism, synthesis of triacylglycerols (TAGs-composed of three FA units esterified to a glycerol backbone-is increased in response to dietary sugar. Saturation in the storage and synthesis capacity of TAGs is associated with type 2 diabetes progression. Sugar toxicity likely depends on advanced-glycation-end-products (AGEs that form through covalent bounding between amine groups and carbonyl groups of sugar or their derivatives α-oxoaldehydes. Methylglyoxal (MG is a highly reactive α-oxoaldehyde that is derived from glycolysis through a non-enzymatic reaction. Glyoxalase 1 (Glo1 works to neutralize MG, reducing its deleterious effects. Here, we have used the power of Drosophila genetics to generate Fatty acid synthase (FASN mutants, allowing us to investigate the consequence of this deficiency upon sugar-supplemented diets. We found that FASN mutants are lethal but can be rescued by an appropriate lipid diet. Rescued animals do not exhibit insulin resistance, are dramatically sensitive to dietary sugar and accumulate AGEs. We show that FASN and Glo1 cooperate at systemic and cell-autonomous levels to protect against sugar toxicity. We observed that the size of FASN mutant cells decreases as dietary sucrose increases. Genetic interactions at the cell-autonomous level, where glycolytic enzymes or Glo1 were manipulated in FASN mutant cells, revealed that this sugar-dependent size reduction is a direct consequence of MG-derived-AGE accumulation. In summary, our findings indicate that FASN is dispensable for cell growth if extracellular lipids are available. In contrast, FA-synthesis appears to be required to limit a cell

  13. Sugar Metabolism in Hummingbirds and Nectar Bats.

    Science.gov (United States)

    Suarez, Raul K; Welch, Kenneth C

    2017-07-12

    Hummingbirds and nectar bats coevolved with the plants they visit to feed on floral nectars rich in sugars. The extremely high metabolic costs imposed by small size and hovering flight in combination with reliance upon sugars as their main source of dietary calories resulted in convergent evolution of a suite of structural and functional traits. These allow high rates of aerobic energy metabolism in the flight muscles, fueled almost entirely by the oxidation of dietary sugars, during flight. High intestinal sucrase activities enable high rates of sucrose hydrolysis. Intestinal absorption of glucose and fructose occurs mainly through a paracellular pathway. In the fasted state, energy metabolism during flight relies on the oxidation of fat synthesized from previously-ingested sugar. During repeated bouts of hover-feeding, the enhanced digestive capacities, in combination with high capacities for sugar transport and oxidation in the flight muscles, allow the operation of the "sugar oxidation cascade", the pathway by which dietary sugars are directly oxidized by flight muscles during exercise. It is suggested that the potentially harmful effects of nectar diets are prevented by locomotory exercise, just as in human hunter-gatherers who consume large quantities of honey.

  14. Sugar Metabolism in Hummingbirds and Nectar Bats

    Directory of Open Access Journals (Sweden)

    Raul K. Suarez

    2017-07-01

    Full Text Available Hummingbirds and nectar bats coevolved with the plants they visit to feed on floral nectars rich in sugars. The extremely high metabolic costs imposed by small size and hovering flight in combination with reliance upon sugars as their main source of dietary calories resulted in convergent evolution of a suite of structural and functional traits. These allow high rates of aerobic energy metabolism in the flight muscles, fueled almost entirely by the oxidation of dietary sugars, during flight. High intestinal sucrase activities enable high rates of sucrose hydrolysis. Intestinal absorption of glucose and fructose occurs mainly through a paracellular pathway. In the fasted state, energy metabolism during flight relies on the oxidation of fat synthesized from previously-ingested sugar. During repeated bouts of hover-feeding, the enhanced digestive capacities, in combination with high capacities for sugar transport and oxidation in the flight muscles, allow the operation of the “sugar oxidation cascade”, the pathway by which dietary sugars are directly oxidized by flight muscles during exercise. It is suggested that the potentially harmful effects of nectar diets are prevented by locomotory exercise, just as in human hunter-gatherers who consume large quantities of honey.

  15. Thermo-mechanical behaviors of thermoplastic starch derived from sugar palm tree (Arenga pinnata).

    Science.gov (United States)

    Sahari, J; Sapuan, S M; Zainudin, E S; Maleque, M A

    2013-02-15

    In recent years, increasing environmental concerns focused greater attention on the development of biodegradable materials. A thermoplastic starch derived from bioresources, sugar palm tree was successfully developed in the presence of biodegradable glycerol as a plasticizer. Sugar palm starch (SPS) was added with 15-40 w/w% of glycerol to prepare workable bioplastics and coded as SPS/G15, SPS/G20, SPS/G30 and SPS/G40. The samples were characterized for thermal properties, mechanical properties and moisture absorption on exposure to humidity were evaluated. Morphological studies through scanning electron microscopy (SEM) were used to explain the observed mechanical properties. Generally, the addition of glycerol decrease the transition temperature of plasticized SPS. The mechanical properties of plasticized SPS increase with the increasing of glycerol but up to 30 w/w%. Meanwhile, the water absorption of plasticized SPS decrease with increasing of glycerol. Copyright © 2012 Elsevier Ltd. All rights reserved.

  16. Root rot diseases of sugar beet

    Directory of Open Access Journals (Sweden)

    Jacobsen Barry J.

    2006-01-01

    Full Text Available Root rot diseases of sugar beet caused by Rhizoctonia solani (AG 2-2 IIIB and AG 2-2 IV, R. crocorum, Aphanomyces cochlioides, Phoma betae, Macrophomina phaeseolina, Fusarium oxysporum f.sp. radicis-betae, Pythium aphanidermatum Phytophthora drechsleri, Rhizopus stolonifer, R. arrhizus and Sclerotium rolfsii cause significant losses wherever sugar beets are grown. However, not all these soil-borne pathogens have been reported in all sugar beet production areas. Losses include reduced harvestable tonnage and reduced white sugar recovery. Many of these pathogens also cause post harvest losses in storage piles. Control for diseases caused by these pathogens include disease resistant cultivars, avoidance of stresses, cultural practices such as water management and the use of fungicides.

  17. THE TECHNOLOGY OF PASTE FROM SUGAR BEET OBTAINING

    Directory of Open Access Journals (Sweden)

    M. G. Magomedov

    2014-01-01

    Full Text Available We considered a new technology for processing sugar beet into intermediate product for the food industry. Nowadays sugar beet is mainly used for processing into granulated sugar. In the granulated sugar obtaining sugar beet undergoes deep extraction of all nutrients: proteins, minerals, pectin, organic acids, preventing the crystallization process, and then the refined product (granulated sugar with a mass fraction of sucrose not less than 99.75% is obtained. We developed a technology for producing a sugar beet paste, which allows to preserve both sucrose, and almost all useful for human food substances containing in beet. A sugar beet paste is a valuable food product. Carbohydrates, organic acids, minerals, proteins, dietary fibers and vitamins are found in it. The block diagram of a sugar beet paste is given in the article. Technology of obtaining a sugar beet paste was tested under production conditions at the cannery (Joint Stock Company "Sadovoye" Liskinsky district,. Storozhevoe village, Voronezh region. The chemical composition of the paste (Dry Substances = 40% was determined, the degree of meeting of daily requirement for nutrients was calculated. 100 g of the product satisfy the daily requirement for dietary fiber by 42.5%, organic acids by 27.5% potassium by 24.0%, magnesium by 40.0%, iron by 26.7%. The sugar beet paste can be used as a semi-finished product in the confectionery, bakery, food concentrates industry, for products with increased nutritional value, as well as the finished product instead of marmalade and jam.

  18. The effect of honey compared to sucrose, mixed sugars, and a sugar-free diet on weight gain in young rats.

    Science.gov (United States)

    Chepulis, L M

    2007-04-01

    To determine whether honey, sucrose, and mixed sugars as in honey have different effects on weight gain, 40 6-wk-old Sprague-Dawley rats were fed a powdered diet that was either sugar free or contained 8% sucrose, 8% mixed sugars as in honey, or 10% honey freely for 6 wk. Weight gain and food intake were assessed weekly, and at completion of the study blood samples were removed for measurement of blood sugar (HbA1c) and a fasting lipid profile. The animals were then minced and total percentage body fat and protein measured. Overall percentage weight gain was significantly lower in honey-fed rats than those fed sucrose or mixed sugars, despite a similar food intake. Weight gains were comparable for rats fed honey and a sugar free diet although food intake was significantly higher in honey-fed rats. HbA1c and triglyceride levels were significantly higher in all sugar treatments compared with rats fed a sugar free diet, but no other differences in lipid profiles were reported. No differences in percentage body fat or protein levels were reported.

  19. Mitochondrial Dysfunction Plus High-Sugar Diet Provokes a Metabolic Crisis That Inhibits Growth.

    Directory of Open Access Journals (Sweden)

    Esko Kemppainen

    Full Text Available The Drosophila mutant tko25t exhibits a deficiency of mitochondrial protein synthesis, leading to a global insufficiency of respiration and oxidative phosphorylation. This entrains an organismal phenotype of developmental delay and sensitivity to seizures induced by mechanical stress. We found that the mutant phenotype is exacerbated in a dose-dependent fashion by high dietary sugar levels. tko25t larvae were found to exhibit severe metabolic abnormalities that were further accentuated by high-sugar diet. These include elevated pyruvate and lactate, decreased ATP and NADPH. Dietary pyruvate or lactate supplementation phenocopied the effects of high sugar. Based on tissue-specific rescue, the crucial tissue in which this metabolic crisis initiates is the gut. It is accompanied by down-regulation of the apparatus of cytosolic protein synthesis and secretion at both the RNA and post-translational levels, including a novel regulation of S6 kinase at the protein level.

  20. Young People\\'s Relationships with Sugar Daddies and Sugar ...

    African Journals Online (AJOL)

    s relationships with sugar daddies and mummies. It considers definitional, measurement and analytical issues involved in assessing these relationships, their magnitude, patterns, determinants and consequences. The review compares and ...

  1. Reducing Sugar in Children's Diets: Why? How?

    Science.gov (United States)

    Rogers, Cosby S.; Morris, Sandra S.

    1986-01-01

    Maintains that sugar intake should be reduced in young children's diets because of its link to dental cavities, poor nutrition, and obesity. Reducing the focus on sweetness, limiting sugar consumption, and using natural sources of sweetness and other treats are ways to help reduce sugar intake. (BB)

  2. Rare sugars and sugar-based synthons by chemo-enzymatic synthesis.

    Science.gov (United States)

    Giffhorn; Köpper; Huwig; Freimund

    2000-12-01

    The unique catalytic potential of the fungal enzyme pyranose oxidase was demonstrated by preparative conversions of a variety of carbohydrates, and by extensive chemical characterization of the reaction products with NMR spectroscopy. The studies revealed that POx not only oxidizes most substrates very efficiently but also that POx possesses a glycosyl-transfer potential, producing disaccharides from beta-glycosides of higher alcohols. Although most substrates are oxidized by POx at the C-2 position, several substrates are converted into the 3-keto-derivatives. On the basis of these products, strategies are developed for the convenient production of sugar-derived synthons, rare sugars and fine chemicals by combining biotechnical and chemical methods.

  3. Composition of sugar cane, energy cane, and sweet sorghum suitable for ethanol production at Louisiana sugar mills.

    Science.gov (United States)

    Kim, Misook; Day, Donal F

    2011-07-01

    A challenge facing the biofuel industry is to develop an economically viable and sustainable biorefinery. The existing potential biorefineries in Louisiana, raw sugar mills, operate only 3 months of the year. For year-round operation, they must adopt other feedstocks, besides sugar cane, as supplemental feedstocks. Energy cane and sweet sorghum have different harvest times, but can be processed for bio-ethanol using the same equipment. Juice of energy cane contains 9.8% fermentable sugars and that of sweet sorghum, 11.8%. Chemical composition of sugar cane bagasse was determined to be 42% cellulose, 25% hemicellulose, and 20% lignin, and that of energy cane was 43% cellulose, 24% hemicellulose, and 22% lignin. Sweet sorghum was 45% cellulose, 27% hemicellulose, and 21% lignin. Theoretical ethanol yields would be 3,609 kg per ha from sugar cane, 12,938 kg per ha from energy cane, and 5,804 kg per ha from sweet sorghum.

  4. 3,6-Anhydro-l-galactose, a rare sugar from agar, a new anticariogenic sugar to replace xylitol.

    Science.gov (United States)

    Yun, Eun Ju; Lee, Ah Reum; Kim, Jung Hyun; Cho, Kyung Mun; Kim, Kyoung Heon

    2017-04-15

    The significance for anticariogenic sugar substitutes is growing due to increasing demands for dietary sugars and rising concerns of dental caries. Xylitol is widely used as an anticariogenic sugar substitute, but the inhibitory effects of xylitol on Streptococcus mutans, the main cause of tooth decay, are exhibited only at high concentrations. Here, the inhibitory effects of 3,6-anhydro-l-galactose (AHG), a rare sugar from red macroalgae, were evaluated on S. mutans, in comparison with those of xylitol. In the presence of 5g/l of AHG, the growth of S. mutans was retarded. At 10g/l of AHG, the growth and acid production by S. mutans were completely inhibited. However, in the presence of xylitol, at a much higher concentration (i.e., 40g/l), the growth of S. mutans still occurred. These results suggest that AHG can be used as a new anticariogenic sugar substitute for preventing dental caries. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. Simulating the Impact of Sugar-Sweetened Beverage Warning Labels in Three Cities.

    Science.gov (United States)

    Lee, Bruce Y; Ferguson, Marie C; Hertenstein, Daniel L; Adam, Atif; Zenkov, Eli; Wang, Peggy I; Wong, Michelle S; Gittelsohn, Joel; Mui, Yeeli; Brown, Shawn T

    2018-02-01

    A number of locations have been considering sugar-sweetened beverage point-of-purchase warning label policies to help address rising adolescent overweight and obesity prevalence. To explore the impact of such policies, in 2016 detailed agent-based models of Baltimore, Philadelphia, and San Francisco were developed, representing their populations, school locations, and food sources, using data from various sources collected between 2005 and 2014. The model simulated, over a 7-year period, the mean change in BMI and obesity prevalence in each of the cities from sugar-sweetened beverage warning label policies. Data analysis conducted between 2016 and 2017 found that implementing sugar-sweetened beverage warning labels at all sugar-sweetened beverage retailers lowered obesity prevalence among adolescents in all three cities. Point-of-purchase labels with 8% efficacy (i.e., labels reducing probability of sugar-sweetened beverage consumption by 8%) resulted in the following percentage changes in obesity prevalence: Baltimore: -1.69% (95% CI= -2.75%, -0.97%, p<0.001); San Francisco: -4.08% (95% CI= -5.96%, -2.2%, p<0.001); Philadelphia: -2.17% (95% CI= -3.07%, -1.42%, p<0.001). Agent-based simulations showed how warning labels may decrease overweight and obesity prevalence in a variety of circumstances with label efficacy and literacy rate identified as potential drivers. Implementing a warning label policy may lead to a reduction in obesity prevalence. Focusing on warning label design and store compliance, especially at supermarkets, may further increase the health impact. Copyright © 2018 American Journal of Preventive Medicine. Published by Elsevier Inc. All rights reserved.

  6. Translation and implementation of added sugars consumption recommendations: a conference report from the American Heart Association Added Sugars Conference 2010.

    Science.gov (United States)

    Van Horn, Linda; Johnson, Rachel K; Flickinger, Brent D; Vafiadis, Dorothea K; Yin-Piazza, Shirley

    2010-12-07

    A 2-day forum was convened to (1) discuss ways to translate the 2009 American Heart Association added sugars recommendations into actions in areas such as regulation, food labeling, nutrient content claims, and practical application in the American diet; (2) review surveillance methodology and metrics for tracking and understanding the impact of reducing added sugars in the diet; and (3) initiate the development of a framework for future collaboration to help Americans implement science-based guidance relative to added sugars. More than 100 multinational participants representing scientists from academia and government and stakeholders engaged in food production, development, and processing, food manufacturing and servicing, food and nutrition policy, and nutrition recommendations for the public attended the conference. Presentations included definitions and examples of added sugars, current US and international added sugars perspectives, added sugars in diets of individuals and in the food supply, food technology behind added sugars, added sugars and health, food manufacturer perspectives, added sugars food-labeling considerations, and examples of positive approaches to improve eating behaviors and the food environment. Facilitated breakout sessions were conducted after the plenary sessions to allow participants to contribute their expertise and thoughts. The American Heart Association Added Sugars Conference is the first step in an important process that facilitates collaboration across science, public health, and industry to foster innovation, partnerships, policy, and implementation of new products and services for the benefit of the health and well-being of the American public. Science has advanced in the area of added sugars and health, creating mounting pressure to use better methods for translation and dissemination of the science for consumer education and for food companies to respond by producing foods and beverages with fewer added sugars. The new

  7. Hierarchy of non-glucose sugars in Escherichia coli.

    Science.gov (United States)

    Aidelberg, Guy; Towbin, Benjamin D; Rothschild, Daphna; Dekel, Erez; Bren, Anat; Alon, Uri

    2014-12-24

    Understanding how cells make decisions, and why they make the decisions they make, is of fundamental interest in systems biology. To address this, we study the decisions made by E. coli on which genes to express when presented with two different sugars. It is well-known that glucose, E. coli's preferred carbon source, represses the uptake of other sugars by means of global and gene-specific mechanisms. However, less is known about the utilization of glucose-free sugar mixtures which are found in the natural environment of E. coli and in biotechnology. Here, we combine experiment and theory to map the choices of E. coli among 6 different non-glucose carbon sources. We used robotic assays and fluorescence reporter strains to make precise measurements of promoter activity and growth rate in all pairs of these sugars. We find that the sugars can be ranked in a hierarchy: in a mixture of a higher and a lower sugar, the lower sugar system shows reduced promoter activity. The hierarchy corresponds to the growth rate supported by each sugar- the faster the growth rate, the higher the sugar on the hierarchy. The hierarchy is 'soft' in the sense that the lower sugar promoters are not completely repressed. Measurement of the activity of the master regulator CRP-cAMP shows that the hierarchy can be quantitatively explained based on differential activation of the promoters by CRP-cAMP. Comparing sugar system activation as a function of time in sugar pair mixtures at sub-saturating concentrations, we find cases of sequential activation, and also cases of simultaneous expression of both systems. Such simultaneous expression is not predicted by simple models of growth rate optimization, which predict only sequential activation. We extend these models by suggesting multi-objective optimization for both growing rapidly now and preparing the cell for future growth on the poorer sugar. We find a defined hierarchy of sugar utilization, which can be quantitatively explained by

  8. Improved sugar yields from biomass sorghum feedstocks: comparing low-lignin mutants and pretreatment chemistries.

    Science.gov (United States)

    Godin, Bruno; Nagle, Nick; Sattler, Scott; Agneessens, Richard; Delcarte, Jérôme; Wolfrum, Edward

    2016-01-01

    For biofuel production processes to be economically efficient, it is essential to maximize the production of monomeric carbohydrates from the structural carbohydrates of feedstocks. One strategy for maximizing carbohydrate production is to identify less recalcitrant feedstock cultivars by performing some type of experimental screening on a large and diverse set of candidate materials, or by identifying genetic modifications (random or directed mutations or transgenic plants) that provide decreased recalcitrance. Economic efficiency can also be increased using additional pretreatment processes such as deacetylation, which uses dilute NaOH to remove the acetyl groups of hemicellulose prior to dilute acid pretreatment. In this work, we used a laboratory-scale screening tool that mimics relevant thermochemical pretreatment conditions to compare the total sugar yield of three near-isogenic brown midrib ( bmr ) mutant lines and the wild-type (WT) sorghum cultivar. We then compared results obtained from the laboratory-scale screening pretreatment assay to a large-scale pretreatment system. After pretreatment and enzymatic hydrolysis, the bmr mutants had higher total sugar yields than the WT sorghum cultivar. Increased pretreatment temperatures increased reactivity for all sorghum samples reducing the differences observed at lower reaction temperatures. Deacetylation prior to dilute acid pretreatment increased the total sugar yield for all four sorghum samples, and reduced the differences in total sugar yields among them, but solubilized a sizable fraction of the non-structural carbohydrates. The general trends of increased total sugar yield in the bmr mutant compared to the WT seen at the laboratory scale were observed at the large-scale system. However, in the larger reactor system, the measured total sugar yields were lower and the difference in total sugar yield between the WT and bmr sorghum was larger. Sorghum bmr mutants, which have a reduced lignin content showed

  9. Urinary sugars biomarker relates better to extrinsic than to intrinsic sugars intake in a metabolic study with volunteers consuming their normal diet.

    Science.gov (United States)

    Tasevska, N; Runswick, S A; Welch, A A; McTaggart, A; Bingham, S A

    2009-05-01

    Sugars in diet are very difficult to measure because of the unreliability of self-reported dietary intake. Sucrose and fructose excreted in urine have been recently suggested as a biomarker for total sugars intake. To further characterize the use of this biomarker, we investigated whether urinary sugars correlated better to extrinsic compared to intrinsic sugars in the diet. Seven male and six female healthy participants were living for 30 days in a metabolic suite under strictly controlled conditions consuming their usual diet as assessed beforehand from four consecutive 7-day food diaries kept at home. During the 30-day study, all 24 h urine specimens were collected, validated for their completeness and analysed for sucrose and fructose. The mean total sugars intake in the group was 202+/-69 g day(-1). Daily intake of extrinsic, intrinsic and milk sugars contributed 60.1, 34.4 and 5.5%, to the total sugars intake, respectively. The individuals' 30-day mean sugars excretion levels were significantly correlated with the 30-day means of extrinsic sugars (r=0.84; Psugars intake (r=0.43; P=0.144). In the regression, only extrinsic sugars intake explained a significant proportion of the variability in sugars excretion (adjusted R(2)=0.64; P=0.001); daily excretion of 100 mg sucrose and fructose in urine predicted 124 g of extrinsic total sugars in the diet. Using fewer urinary and dietary measurements in the analysis did not change the overall trend of the findings. In this group of volunteers, sucrose and fructose in urine better correlated to extrinsic than to intrinsic sugars intake.

  10. Eh and fission product solubilities: two factors in the leaching of UO2

    International Nuclear Information System (INIS)

    Ogard, A.E.; Duffy, C.J.

    1981-01-01

    Eh was found to have a large effect on the dissolution of UO 2 in water at pH 4. As was estimated from thermodynamic data, the solubility was found to decrease as the oxygen fugacity, and therefore the Eh of the water, was decreased. Some of the rare earths and other actinides such as europium, cerium, americium, and plutonium released during the leaching of a spent fuel element behaved differently. These elements were not affected to any large extent by the variation in Eh of these experiments. It has been postulated that these elements reached their solubility limits and precipitated as the spent fuel was leached. 2 figures, 2 tables

  11. The effect of organosolv pretreatment on optimization of hydrolysis process to produce the reducing sugar

    Directory of Open Access Journals (Sweden)

    Lini Fibrillian Zata

    2018-01-01

    Full Text Available As the fossil energy decrease such as petroleum and natural gas, that are encourages a lot of research to develop new sources of energy from renewable raw materials. One of the source is through reducing sugar (glucose and xylose obtained from coffee pulp waste; this is due to abundant production of coffee pulp every year reaching 743 kg/ha. In addition, this waste has not been used optimally and the cellulose and hemicellulose content of the coffee is high. The purpose of this study is to get the optimal operating condition for reducing sugar production from coffee pulp waste. The method used for optimization is Response Surface Methodology with Central Composite Design. The optimum operation condition obtained was pH 4.63 at 34ºC for 16.29 hours of hydrolysis. As a result, the predicted yield gained was 0.147 grams of reducing sugars / gram of cellulose+hemicellulose. The result indicates the gained yield was 0.137 grams of reducing sugars / gram of cellulose+hemicellulose.

  12. Solubilities of benzoic acid in binary (benzyl alcohol + benzaldehyde) solvent mixtures

    International Nuclear Information System (INIS)

    Wang, Hui; Wang, Qinbo; Xiong, Zhenhua; Chen, Chuxiong; Shen, Binwei

    2015-01-01

    Highlights: • Solubilities of benzoic acid in (benzyl alcohol + benzaldehyde) mixtures were measured at 1 atm. • The experimental temperature ranges at (298.35 to 355.65) K. • Effects of benzyl alcohol mass concentration at (0.00 to 1.00) on the solubilities of benzoic acid were studied. • The experimental data were correlated with NRTL model. • Thermodynamic functions of dissolution of benzoic acid in (benzyl alcohol + benzaldehyde) mixtures were discussed. - Abstract: The solubility of benzoic acid in binary (benzyl alcohol + benzaldehyde) solvent mixtures was measured at temperature from (298.35 to 355.65) K and atmospheric pressure. The measured solubility increases with the increasing temperature at constant solvent composition. The effects of mass fraction benzaldehyde in the solvent mixtures at (0.0 to 1.00) on the solubility were studied. The measured solubility decreases with the increasing mass fraction of benzaldehyde. The experimental results were correlated with the non-random two-liquid (NRTL) equations, and good agreement between the correlated and the experimental values was obtained. Thermodynamic functions for the solution of benzoic acid in binary (benzyl alcohol + benzaldehyde) solvent mixtures were calculated with the van’t Hoff plot. The apparent dissolution Gibbs free energy change was also calculated

  13. Toll-like receptor 4 mediates fat, sugar, and umami taste preference and food intake and body weight regulation.

    Science.gov (United States)

    Camandola, Simonetta; Mattson, Mark P

    2017-07-01

    Immune and inflammatory pathways play important roles in the pathogenesis of metabolic disorders. This study investigated the role of toll-like receptor 4 (TLR4) in orosensory detection of dietary lipids and sugars. Taste preferences of TLR4 knockout (KO) and wild-type (WT) male mice under a standard and a high-fat, high-sugar diet were assessed with two-bottle tests. Gene expression of taste signaling molecules was analyzed in the tongue epithelium. The role of TLR4 in food intake and weight gain was investigated in TLR4 KO and WT mice fed a high-fat and high-sugar diet for 12 weeks. Compared to WT mice, TLR4 KO mice showed reduced preference for lipids, sugars, and umami in a two-bottle preference test. The altered taste perception was associated with decreased levels of key taste regulatory molecules in the tongue epithelium. TLR4 KO mice on a high-fat and high-sugar diet consumed less food and drink, resulting in diminished weight gain. TLR4 signaling promotes ingestion of sugar and fat by a mechanism involving increased preference for such obesogenic foods. © 2017 The Obesity Society.

  14. Sugars, exercise and health.

    Science.gov (United States)

    Codella, Roberto; Terruzzi, Ileana; Luzi, Livio

    2017-12-15

    There is a direct link between a variety of addictions and mood states to which exercise could be relieving. Sugar addiction has been recently counted as another binge/compulsive/addictive eating behavior, differently induced, leading to a high-significant health problem. Regularly exercising at moderate intensity has been shown to efficiently and positively impact upon physiological imbalances caused by several morbid conditions, including affective disorders. Even in a wider set of physchiatric diseases, physical exercise has been prescribed as a complementary therapeutic strategy. A comprehensive literature search was carried out in the Cochrane Library and MEDLINE databases (search terms: sugar addiction, food craving, exercise therapy, training, physical fitness, physical activity, rehabilitation and aerobic). Seeking high-sugar diets, also in a reward- or craving-addiction fashion, can generate drastic metabolic derangements, often interpolated with affective disorders, for which exercise may represent a valuable, universal, non-pharmachological barrier. More research in humans is needed to confirm potential exercise-mechanisms that may break the bond between sugar over-consumption and affective disorders. The purpose of this review is to address the importance of physical exercise in reversing the gloomy scenario of unhealthy diets and sedentary lifestyles in our modern society. Copyright © 2016 Elsevier B.V. All rights reserved.

  15. СHIPS FROM SUGAR BEET

    Directory of Open Access Journals (Sweden)

    G. O. Magomedov

    2014-01-01

    Full Text Available Summary. Priority social problem in the Russian Federation is to provide diverse populations rational healthy diet, taking into account their traditions and economic status. Solving this problem requires the development of processing industries of agriculture on the basis of the improvement of existing and creation of new energy-saving environmentally friendly technologies that can provide deep, if possible without waste, recycling of raw materials. Therefore, the aim of research was the development of technology for production of sugar beet chips. Technology is as follows: sugar beet supplied into the washing machine to remove dirt from its surface. Washed roots inspect on conveyor belts. Next pure sugar beets sent to steam-heat treatment for cleaning the skin. After the beets is subjected to cutting by combining this process with a treatment with an aqueous solution of citric acid. Then he sent for the drying process is completed upon reaching a product of moisture content of 4-5 %. Drying chips feature is that under the high temperature reaction proceeds melanoidins between proteins and sugars present in sugar beet. As a result, the product obtained has the following characteristics: gold-yellow color; absence of a characteristic odor of sugar beet; pleasant sour taste; humidity of 4-5%. Thus, the new technology is relevant, because now the chips are one of the most popular products, ready to eat. A beet chips are rich in dietary fiber (pectin, hemicellulose and cellulose - 4-5 % minerals - macroelements (potassium, sodium, magnesium, calcium, phosphorus, trace elements (iron, zinc, copper, manganese - 0.5-0.6 %, and are the product of a functional food.

  16. Total antioxidant content of alternatives to refined sugar.

    Science.gov (United States)

    Phillips, Katherine M; Carlsen, Monica H; Blomhoff, Rune

    2009-01-01

    Oxidative damage is implicated in the etiology of cancer, cardiovascular disease, and other degenerative disorders. Recent nutritional research has focused on the antioxidant potential of foods, while current dietary recommendations are to increase the intake of antioxidant-rich foods rather than supplement specific nutrients. Many alternatives to refined sugar are available, including raw cane sugar, plant saps/syrups (eg, maple syrup, agave nectar), molasses, honey, and fruit sugars (eg, date sugar). Unrefined sweeteners were hypothesized to contain higher levels of antioxidants, similar to the contrast between whole and refined grain products. To compare the total antioxidant content of natural sweeteners as alternatives to refined sugar. The ferric-reducing ability of plasma (FRAP) assay was used to estimate total antioxidant capacity. Major brands of 12 types of sweeteners as well as refined white sugar and corn syrup were sampled from retail outlets in the United States. Substantial differences in total antioxidant content of different sweeteners were found. Refined sugar, corn syrup, and agave nectar contained minimal antioxidant activity (sugar had a higher FRAP (0.1 mmol/100 g). Dark and blackstrap molasses had the highest FRAP (4.6 to 4.9 mmol/100 g), while maple syrup, brown sugar, and honey showed intermediate antioxidant capacity (0.2 to 0.7 mmol FRAP/100 g). Based on an average intake of 130 g/day refined sugars and the antioxidant activity measured in typical diets, substituting alternative sweeteners could increase antioxidant intake an average of 2.6 mmol/day, similar to the amount found in a serving of berries or nuts. Many readily available alternatives to refined sugar offer the potential benefit of antioxidant activity.

  17. European Sugar Market – Impact of Quota System

    Directory of Open Access Journals (Sweden)

    Irena Benešová

    2015-01-01

    Full Text Available The European agricultural market has been criticized for its heavy regulations and subsidization. The sugar market is one of the most regulated ones; however, this will change radically in 2017 when the current system of production quotas will end. The aim of this paper is to present the basic characteristics of the EU quota sugar market. The analysis identifies the main drivers of EU sugar market and their position within the EU sugar market. The paper identifies especially those drivers/companies/alliances which take control over the EU sugar production realized under the quota production system. The paper also highlights the level of EU sugar market concentration and also identifies those countries and companies which are the main leaders in the sugar production area realized under the quota system. Based on the results deriving from the paper, it is possible to characterize the EU sugar market as a heavily concentrated one – nearly 75% (10 mil. tonnes of the quota is controlled by five multinational companies only; these companies are operating more than 50% of all the available sugar plants located in the EU. These multinational alliances are also in control of the production capacities of their subsidiaries. In most countries, this causes serious problems as the given quota is controlled by one or two producers only. The EU sugar market is extremely concentrated especially if we take into consideration the location of each alliance’s headquarters. The majority of production capacities are under (the control of especially German and French companies. These two countries are also the main beneficiaries in relation to the EU sugar production quota system.

  18. Temporal variability in trace metal solubility in a paddy soil not reflected in uptake by rice (Oryza sativa L.).

    Science.gov (United States)

    Pan, Yunyu; Koopmans, Gerwin F; Bonten, Luc T C; Song, Jing; Luo, Yongming; Temminghoff, Erwin J M; Comans, Rob N J

    2016-12-01

    Alternating flooding and drainage conditions have a strong influence on redox chemistry and the solubility of trace metals in paddy soils. However, current knowledge of how the effects of water management on trace metal solubility are linked to trace metal uptake by rice plants over time is still limited. Here, a field-contaminated paddy soil was subjected to two flooding and drainage cycles in a pot experiment with two rice plant cultivars, exhibiting either high or low Cd accumulation characteristics. Flooding led to a strong vertical gradient in the redox potential (Eh). The pH and Mn, Fe, and dissolved organic carbon concentrations increased with decreasing Eh and vice versa. During flooding, trace metal solubility decreased markedly, probably due to sulfide mineral precipitation. Despite its low solubility, the Cd content in rice grains exceeded the food quality standards for both cultivars. Trace metal contents in different rice plant tissues (roots, stem, and leaves) increased at a constant rate during the first flooding and drainage cycle but decreased after reaching a maximum during the second cycle. As such, the high temporal variability in trace metal solubility was not reflected in trace metal uptake by rice plants over time. This might be due to the presence of aerobic conditions and a consequent higher trace metal solubility near the root surface, even during flooding. Trace metal solubility in the rhizosphere should be considered when linking water management to trace metal uptake by rice over time.

  19. Dietary sources of sugars in adolescents' diet: the HELENA study.

    Science.gov (United States)

    Mesana, M I; Hilbig, A; Androutsos, O; Cuenca-García, M; Dallongeville, J; Huybrechts, I; De Henauw, S; Widhalm, K; Kafatos, A; Nova, E; Marcos, A; González-Gross, M; Molnar, D; Gottrand, F; Moreno, L A

    2018-03-01

    To report dietary sugars consumption and their different types and food sources, in European adolescents. Food consumption data of selected groups were obtained from 1630 adolescents (45.6% males, 12.5-17.5 years) from the HELENA study using two nonconsecutive 24-h recalls. Energy intake, total sugars and free sugars were assessed using the HELENA-DIAT software. Multiple regression analyses were performed adjusting for relevant confounders. Total sugars intake (137.5 g/day) represented 23.6% and free sugars (110.1 g/day), 19% of energy intake. Girls had significantly lower intakes of energy, carbohydrates, total sugars and free sugars. 94% of adolescents had a consumption of free sugars above 10% of total energy intake. The main food contributor to free sugars was 'carbonated, soft and isotonic drinks,' followed by 'non-chocolate confectionary' and 'sugar, honey, jam and syrup.' Older boys and girls had significantly higher intakes of free sugars from 'cakes, pies and biscuits.' Free sugars intake was negatively associated with low socioeconomic status for 'non-chocolate confectionary' and 'sugar, honey and jam' groups; with low maternal educational level for carbonated and 'soft drinks,' 'sugar, honey and jam,' 'cakes and pies' and 'breakfast cereals' groups; and with high paternal educational level for 'carbonated and soft drinks' and 'chocolates' group. The majority (94%) of studied adolescents consumed free sugars above 10% of daily energy intake. Our data indicate a broad variety in foods providing free sugars. Continued efforts are required at different levels to reduce the intake of free sugars, especially in families with a low educational level.

  20. Effect of Cuscuta (Dodder on Quality and Quantity Traits of Sugar Beet in Chenaran, Khorasan Razavi Province

    Directory of Open Access Journals (Sweden)

    sh Amirmoradi

    2012-02-01

    Full Text Available Abstract Dodder is an annual parasitic plant that has not chlorophyll. This parasitic plant can attach to many field crops such as sugar beet. Also this parasite consumes some water, nutrients and assimilates from plants which result in decreasing of quality and quantity of crops yield. This study carried out in Chenaran county, Khorasan Razavi province, in order to evaluation of damage effects of dodder on qualitative and quantitative traits of sugar beet in 2006. Three fields which naturally formerly have infested by dodder, selected in three locations (Ghezlar, Masi Hazrati and Moghan. Sowing date was nearly in April in all fields. At the harvest time, in November, in each field 20 sample of sugar beet roots separately were harvested. Harvest area of each sample was 8m2. Each sample selected from spots which indicated nearly 80-100 percentage infestation. Observations were paired samples and then 20 samples of uninfested spots (with zero percentage infestation at neighboring rows of infested spots with the same area (8m2 were harvested. Samples were analyzed with qualitative and quantitative standard methods. Obtained data were analyzed by SAS Software with t test and samples compaired in pairwise comparisons. Results indicated that dodder in all sugar beet fields reduced root yield (RY, sugar content(SC, sugar yield (SY, white sugar content (WSC, yield, white sugar yield (WSY, 15.07% , 1.06 Unit, 20.35 %, 7.40 %, 1.89 Unit and 17.73%, respectively. Root potassium content and harmful nitrogen content on field infested spots reduced (8.55% and 8.02 % respectively, but Na content increased 24.3% and molasses Sugar content (MS increased 11.16 %. Conclusion from this experiment showed that dodder damage and its harmful effects were highly significant in all farms in Chenaran. Therefore consideration of field sanitation related to attachment of dodder to sugar beet is completely necessary. Also it had better consider methods of dodder control