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Sample records for snapper pagrus auratus

  1. Infection with Photobacterium damselae subspecies damselae and Vibrio harveyi in snapper, Pagrus auratus with bloat.

    Science.gov (United States)

    Stephens, F J; Raidal, S R; Buller, N; Jones, B

    2006-05-01

    To diagnose the cause of chronic, low mortality associated with bloat in tanks of snapper at an aquaculture facility. A clinical, pathological and microbiological investigation into the cause of a low number of ongoing mortalities associated with bloat in snapper at an aquaculture facility is outlined. Necropsy, histology, microbiology and a comparison of haematology and water analysis from affected and unaffected fish and holding tanks, respectively were conducted. Affected moribund fish were found in lateral or dorsal recumbency floating on the water surface within 24 hours of death. Photobacterium damselae subspecies damselae was isolated from intestinal contents and Vibrio harveyi from the blood of affected fish and both were isolated from culture water. Both V harveyi and P damselae subspecies damselae isolates were sensitive to tetracycline, ciprofloxacin and sulphamethoxazole plus trimethoprim. Environmental parameters such as pH and dissolved oxygen were similar in tanks of affected and unaffected fish. Affected fish had gas distended swimbladders, anaemia, and the intestines were diffusely distended with a clear, pale yellowish fluid. Livers were mottled tan and green in a zonal pattern. Histologically the intestines of fish from tanks suffering mortality had a moderate granulocytic enteritis with oedema and infiltrations with eosinophilic granule cells that were also present as an infiltrate in the gills. There were elevated numbers of melanomacrophage centres and haemosiderin deposits in the spleen, kidney and liver of affected fish. Vibrio harveyi and Photobacterium damselae subspecies damselae infection should be recognised as potential pathogens of snapper held in water of less than optimal quality.

  2. Annual, lunar and diel reproductive periodicity of a spawning aggregation of snapper Pagrus auratus (Sparidae) in a marine embayment on the lower west coast of Australia.

    Science.gov (United States)

    Wakefield, C B

    2010-10-01

    Ichthyoplankton sampling and ovarian characteristics were used to elucidate whether the reproductive cycles of a spawning aggregation of snapper Pagrus auratus in a nearshore marine embayment were temporally and spatially specific and related with environmental conditions. The reproductive dynamics of this aggregation were studied over four consecutive years (2001-2004). Spawning occurred between September and January each year, when water temperatures ranged from 15·8 to 23·1° C. In all 4 years, the cumulative egg densities in Cockburn Sound were highest when water temperatures were between the narrow range of 19-20° C. The spawning fraction of females was monthly bimodal and peaked during new and the full moons at 96-100% and c. 75%, respectively. The backcalculated ages of P. auratus eggs collected from 16 ichthyoplankton surveys demonstrated that P. auratus in Cockburn Sound spawn at night during the 3 h following the high tide. The spatial distributions of P. auratus eggs in Cockburn Sound during the peak reproductive period in all 4 years were consistent, further implying spawning was temporally and spatially specific. High concentrations of recently spawned eggs (8-16 h old) demonstrated spawning also occurred within the adjacent marine embayments of Owen Anchorage and Warnbro Sound. Water circulation in Cockburn and Warnbro Sounds resembled an eddy that was most prominent during the period of highest egg densities, thereby facilitating the retention of eggs in these areas. The reproductive cycles of P. auratus described in this study have assisted managers with the appropriate temporal and spatial scale for a closed fishing season to protect these spawning aggregations. © 2010 The Author. Journal compilation © 2010 The Fisheries Society of the British Isles.

  3. The acute stress response of red porgy, Pagrus pagrus, kept on a red or white background

    NARCIS (Netherlands)

    Salm, A.L. van der; Pavlidis, M; Flik, G.; Wendelaar Bonga, S.E.

    2005-01-01

    The skin colour of red porgy, Pagrus pagrus, can be modified by exposure to different background colours. Red and white background colours brighten the dark skin colour that develops under common culture conditions in red porgy. To assess whether skin colour is also modified by aquaculture related

  4. Effects of husbandry conditions on the skin colour and stress response of red porgy, Pagrus pagrus

    NARCIS (Netherlands)

    Salm, A.L. van der; Martinez, M.; Flik, G.; Wendelaar Bonga, S.E.

    2004-01-01

    Red porgy, Pagrus pagrus, is a potential candidate for aquaculture. However, darkening of the body occurs after capture of wild fish and during fanning of cultured animals. In fish, skin pigmentation is hormonally controlled and the main hormone involved in skin darkening,

  5. The use of morphological and histological features as nutritional condition indices of Pagrus pagrus larvae

    Directory of Open Access Journals (Sweden)

    Marina Vera Diaz

    Full Text Available Morphometrical and histological techniques were employed to characterize Pagrus pagrus larvae nutritional condition. Larvae were reared in laboratory under controlled conditions with the main objective of testing whether these methodologies allowed finding differences between larvae from different feeding treatments. Once yolk was consumed (three days after hatching larvae were assigned to a feeding treatment: starved during the whole experiment; delayed feeding, starved during three days; fed during the entire experiment. Algae (Nannochloropsis oculata and rotifers (Brachionus plicatilis were provided to larvae for feed treatments. Larvae were fixed daily; for morphometrical purposes in 5% formaldehyde solution, and in Bouin for histological sections. Results herein obtained showed that both methodologies are sensitive enough to distinguish larvae characterized by different nutritional condition states obtained from the feeding treatments. Consequently, these methodologies could be employed in wild red porgy larvae in order to asses their nutritional condition. These techniques could also be employed to check larval quality obtained with aquaculture purposes to estimate the effects of changes in rearing protocols or kind of food supply and thus, to guaranty a higher survival of early developmental stages of reared larvae.

  6. Can Lutjanus purpureus (South red snapper be "legally" considered a red snapper (Lutjanus campechanus?

    Directory of Open Access Journals (Sweden)

    Grazielle Gomes

    2008-01-01

    Full Text Available Red snappers (Lutjanus purpureus in Brazil and Lutjanus campechanus in USA and Gulf of Mexico are both under clear effect of overfishing. Because of their high morphological similarity it has already been suggested that they could possibly be considered as a single species. To investigate the degree of similarity and the genetic structure of red snapper populations we constructed a common dataset of partial D-loop mtDNA sequences of L. purpureus from Brazil (Amapá, Pará and Maranhão and L. campechanus from the Atlantic coast of the USA (Florida, Louisiana and Mississippi. Phylogenetic and population genetic analyses surprisingly depicted high similarity between L. campechanus and L. purpureus, compatible with the hypothesis of a single species of red snapper for the Western Atlantic Ocean. These preliminary but very curious findings open an important discussion regarding the legislation involved on the capture of this overexploited fish resources as well as regarding their taxonomy.

  7. Effect of diet on the feces quality in javan langur (Trachypithecus auratus auratus)

    OpenAIRE

    Nijboer, Joeke; Clauss, Marcus; Olsthoorn, M; Noordermeer, W; Huisman, T R; Verheyen, C; van der Kuilen, J; Streich, W J; Beynen, A C

    2006-01-01

    A high intake of easily fermentable carbohydrates and a low intake of fiber material are generally regarded as major factors affecting the health of captive langurs. The effect on fecal consistency of excluding fruits and vegetables from the diet was evaluated in Javan langurs (Trachypithecus auratus auratus). Cross-over trials were carried out at Rotterdam Zoo and at the Apenheul Zoo, The Netherlands. During the first and third dietary period, the langurs were fed their usual diet, which con...

  8. 78 FR 49183 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2013-08-13

    ... 15. The purpose of Regulatory Amendment 15 and this final rule is to provide socio-economic benefits... griseus Mahogany snapper, Lutjanus mahogoni Dog snapper, Lutjanus jocu Lane snapper, Lutjanus synagris...

  9. Cryopreservation of mutton snapper ( Lutjanus analis sperm

    Directory of Open Access Journals (Sweden)

    EDUARDO G. SANCHES

    2013-09-01

    Full Text Available This study aimed to develop a protocol of semen cryopreservation of the mutton snapper Lutjanus analis. The interaction between three extenders ( pH 6.1; 7.8 and 8.2 , two concentrations of dimethyl sulfoxide ( DMSO, 5 and 10% and three cooling rates ( -90; -60 and -30°C.min−1 on the sperm motility rate and motility time were analyzed by a factorial experiment. A sample of 30 fishes ( 1,261 ± 449 g collected in the nature was kept in floating net cages. The semen was frozen by using cryogenic straws, in nitrogen vapour and transferred, later, to liquid nitrogen. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The highest sperm motility rate and motility time ( P < 0.05 was achieved by combining extender C ( pH 8.2 with DMSO ( 10% and cooling rate of -60°C.min−1 ( P < 0.05 . The use of cryopreserved sperm presented fertilization rates higher than 59% validating the present protocol for mutton snapper.

  10. 2010 FL-GA red snapper longline CRP project

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data are from a cooperative effort between scientists and commercial fishers to assess whether a cryptic biomass of relatively old and large red snapper...

  11. On the record of Red seabream Pagrus major (Temminck and Schlegel,1843 (Osteichthyes: Sparidae in the Adriatic Sea

    Directory of Open Access Journals (Sweden)

    Jakov Dulcic

    2007-03-01

    Full Text Available One specimen of red seabream Pagrus major, 44.9 cm total length, was caught in the eastern middle Adriatic (Island Molat, Cape Bonaster by long-line hook at a depth of about 20 m on a hard rocky bottom on 25 September 2004. It is the first record of this species in the Adriatic Sea and Mediterranean area.

  12. 2012 GA-SC-NC red snapper multi-gear CRP project

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Surveys were completed at 1,656 locations, yielding 173 red snapper length and 159 red snapper age samples. Catches were greatest in waters off GA (n 133), and...

  13. 78 FR 15672 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2013-03-12

    ... (SEDAR) benchmark stock assessment for red snapper, SEDAR 24, was completed in October 2010. Much like... found in the 2008 stock assessment. The next benchmark stock assessment for red snapper is scheduled for... red snapper stock and to provide socio-economic benefits to snapper-grouper fishermen and communities...

  14. Ethnobiology of snappers (Lutjanidae: target species and suggestions for management

    Directory of Open Access Journals (Sweden)

    Clauzet Mariana

    2011-03-01

    Full Text Available Abstract In this study, we sought to investigate the biology (diet and reproduction and ethnobiology (fishers knowledge and fishing spots used to catch snappers of five species of snappers (Lutjanidae, including Lutjanus analis, Lutjanus synagris, Lutjanus vivanus, Ocyurus chrysurus, and Romboplites saliens at five sites along the northeast (Riacho Doce, Maceió in Alagoas State, and Porto do Sauípe, Entre Rios at Bahia State and the southeast (SE Brazilian coast (Paraty and Rio de Janeiro cities at Rio de Janeiro State, and Bertioga, at São Paulo State.. We collected 288 snappers and interviewed 86 fishermen. The stomach contents of each fish were examined and macroscopic gonad analysis was performed. Snappers are very important for the fisheries of NE Brazil, and our results indicated that some populations, such as mutton snapper (L. analis and lane snapper (L. synagris, are being caught when they are too young, at early juvenile stages. Local knowledge has been shown to be a powerful tool for determining appropriate policies regarding management of target species, and artisanal fishermen can be included in management processes. Other suggestions for managing the fisheries are discussed, including proposals that could provide motivation for artisanal fishermen to participate in programs to conserve resources, such as co-management approaches that utilize local knowledge, the establishment of fishing seasons, and compensation of fishermen, through 'payment for environmental services'. These suggestions may enhance the participation of local artisanal fishermen in moving to a more realistic and less top-down management approach of the fish population.

  15. Ethnobiology of snappers (Lutjanidae): target species and suggestions for management.

    Science.gov (United States)

    Begossi, Alpina; Salivonchyk, Svetlana V; Araujo, Luciana G; Andreoli, Tainá B; Clauzet, Mariana; Martinelli, Claudia M; Ferreira, Allan G I; Oliveira, Luiz E C; Silvano, Renato A M

    2011-03-16

    In this study, we sought to investigate the biology (diet and reproduction) and ethnobiology (fishers knowledge and fishing spots used to catch snappers) of five species of snappers (Lutjanidae), including Lutjanus analis, Lutjanus synagris, Lutjanus vivanus, Ocyurus chrysurus, and Romboplites saliens at five sites along the northeast (Riacho Doce, Maceió in Alagoas State, and Porto do Sauípe, Entre Rios at Bahia State) and the southeast (SE) Brazilian coast (Paraty and Rio de Janeiro cities at Rio de Janeiro State, and Bertioga, at São Paulo State.).We collected 288 snappers and interviewed 86 fishermen. The stomach contents of each fish were examined and macroscopic gonad analysis was performed. Snappers are very important for the fisheries of NE Brazil, and our results indicated that some populations, such as mutton snapper (L. analis) and lane snapper (L. synagris), are being caught when they are too young, at early juvenile stages.Local knowledge has been shown to be a powerful tool for determining appropriate policies regarding management of target species, and artisanal fishermen can be included in management processes. Other suggestions for managing the fisheries are discussed, including proposals that could provide motivation for artisanal fishermen to participate in programs to conserve resources, such as co-management approaches that utilize local knowledge, the establishment of fishing seasons, and compensation of fishermen, through 'payment for environmental services'. These suggestions may enhance the participation of local artisanal fishermen in moving to a more realistic and less top-down management approach of the fish population.

  16. Dietary vitamin E on the reproductive performance of the fantail goldfish Carassius auratus auratus

    Directory of Open Access Journals (Sweden)

    Harton Arfah

    2014-01-01

    Full Text Available ABSTRACT This research was conducted to examine the different dose of vitamin E in the diet on female broodstock reproduction performance of the fantail goldfish Carassius auratus auratus. This research consisted of four treatments with three replications. The use of vitamin E doses was 0, 125, 250, and 375 mg/kg. The vitamin E was dissolved in vegetable oil and mixed with albumen as a binder in feed. The vitamin E was sprayed at feed and was air dried. Female broodstock of the fantail goldfishes were reared for 40 days. The result showed that 375 mg/kg treatment performed the highest quality of reproduction. Egg diameter, gonadosomatic index, fecundity, and germinal vesicle breakdown of fish which are treated by 375 mg/kg vitamin E were respectively 0.92±0.05 mm, 8.86±4.62%, 56.00±29.18%, and 67.35±17.67% higher than control. Thus, 375 mg/kg of dietary vitamin E suplementating was a best dose to improve female broodstock productivity of the fantail goldfish Keywords: female broodstock, fantail goldfish, vitamin E, reproduction quality  ABSTRAK Penelitian dilakukan untuk menguji suplementasi vitamin E dengan dosis berbeda dicampur ke dalam pakan komersial terhadap produktivitas induk betina ikan komet Carassius auratus auratus. Penelitian ini menggunakan empat perlakuan dengan tiga ulangan. Dosis vitamin E yang diberikan, yaitu 0, 125, 250, dan 375 mg/kg pakan. Vitamin E dilarutkan dalam minyak nabati dan dicampur dengan putih telur sebagai perekat pada pakan. Vitamin E disemprotkan ke pakan dan dikeringanginkan. Induk betina ikan komet pascasalin dengan bobot 72,78±19,47 g diberi perlakuan selama 40 hari. Hasil penelitian menunjukkan bahwa induk betina ikan komet yang diberi suplementasi vitamin E sebanyak 375 mg/kg dalam pakan memiliki diameter telur 0,92±0,05 mm, gonadosomatic index 8,86±4,62%, fekunditas 56,00±29,18 butir/g ikan, dan germinal vesicle breakdown 67,35±17,67% yang lebih tinggi dibandingkan kontrol. Dengan demikian

  17. 75 FR 27658 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery of the...

    Science.gov (United States)

    2010-05-18

    ... reduce overfishing of red snapper in the South Atlantic implemented by a temporary rule published by NMFS... reduce overfishing of red snapper in the South Atlantic. DATES: The effective date for the interim rule... extension of the rule is to reduce the overfishing of red snapper while long-term management measures are...

  18. 78 FR 25047 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2013-04-29

    ... the Stock The most recent Southeast Data, Assessment, and Review (SEDAR) benchmark stock assessment.... The next benchmark stock assessment for red snapper is scheduled for 2014. Measures Contained in This... continue the rebuilding of the red snapper stock and to provide socio-economic benefits to snapper-grouper...

  19. 75 FR 76890 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2010-12-09

    ... 17A. A Southeast Data Assessment and Review (SEDAR) benchmark stock assessment for red snapper (SEDAR... time to respond to new scientific information. Based upon the results of the SEDAR 24 benchmark stock... of overfishing of the red snapper stock. The SEDAR 24 benchmark stock assessment for red snapper was...

  20. 76 FR 23728 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2011-04-28

    ... determined through a Southeast Data Assessment and Review (SEDAR) benchmark stock assessment for red snapper... or spearfishing gear for species other than red snapper. Through the SEDAR 24 benchmark stock.... The SEDAR 24 assessment determined, similar to the SEDAR 15 benchmark, that the red snapper stock is...

  1. 76 FR 78879 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2011-12-20

    ..., recent analysis of landings data (June 1, 2011, SERO-LAPP- 2011-06 Report) indicate that speckled hind... shelf break. Blueline tilefish, which is targeted for harvest by the deep-water component of the snapper.... Even though yellowedge grouper, misty grouper, queen snapper, and silk snapper primarily share the same...

  2. Species distribution models of tropical deep-sea snappers.

    Directory of Open Access Journals (Sweden)

    Céline Gomez

    Full Text Available Deep-sea fisheries provide an important source of protein to Pacific Island countries and territories that are highly dependent on fish for food security. However, spatial management of these deep-sea habitats is hindered by insufficient data. We developed species distribution models using spatially limited presence data for the main harvested species in the Western Central Pacific Ocean. We used bathymetric and water temperature data to develop presence-only species distribution models for the commercially exploited deep-sea snappers Etelis Cuvier 1828, Pristipomoides Valenciennes 1830, and Aphareus Cuvier 1830. We evaluated the performance of four different algorithms (CTA, GLM, MARS, and MAXENT within the BIOMOD framework to obtain an ensemble of predicted distributions. We projected these predictions across the Western Central Pacific Ocean to produce maps of potential deep-sea snapper distributions in 32 countries and territories. Depth was consistently the best predictor of presence for all species groups across all models. Bathymetric slope was consistently the poorest predictor. Temperature at depth was a good predictor of presence for GLM only. Model precision was highest for MAXENT and CTA. There were strong regional patterns in predicted distribution of suitable habitat, with the largest areas of suitable habitat (> 35% of the Exclusive Economic Zone predicted in seven South Pacific countries and territories (Fiji, Matthew & Hunter, Nauru, New Caledonia, Tonga, Vanuatu and Wallis & Futuna. Predicted habitat also varied among species, with the proportion of predicted habitat highest for Aphareus and lowest for Etelis. Despite data paucity, the relationship between deep-sea snapper presence and their environments was sufficiently strong to predict their distribution across a large area of the Pacific Ocean. Our results therefore provide a strong baseline for designing monitoring programs that balance resource exploitation and

  3. Effect of diet on the feces quality in javan langur (Trachypithecus auratus auratus).

    Science.gov (United States)

    Nijboer, Joeke; Clauss, Marcus; Olsthoorn, Moniek; Noordermeer, Wendy; Huisman, Tjalling R; Verheyen, Celine; van der Kuilen, Jan; Jürgen, W Streich; Beynen, Anton C

    2006-09-01

    A high intake of easily fermentable carbohydrates and a low intake of fiber material are generally regarded as major factors affecting the health of captive langurs. The effect on fecal consistency of excluding fruits and vegetables from the diet was evaluated in Javan langurs (Trachypithecus auratus auratus). Cross-over trials were carried out at Rotterdam Zoo and at the Apenheul Zoo, The Netherlands. During the first and third dietary period, the langurs were fed their usual diet, which contained fruits, vegetables, langur pellets, and browse. During the second period, the vegetables and fruits were excluded from the diet and the diet essentially consisted of pellets and browse. Feces consistency was scored using a fecal score chart developed for langurs. During the second feeding period the feces consistency improved significantly in animals at both zoos. Across all trials, a firmer feces consistency was correlated with an increase in dietary cell wall (measured as neutral detergent fiber) and a decrease in dietary water. It is suggested that the combined decrease in the intake of soluble sugars, the increase of fiber intake, and a lower amount of dietary water in the diet resulted in more solid stools. The results indicate that a dietary neutral detergent fiber content of approximately 46% in dry matter will result in a feces consistency indicative of undisturbed gut function.

  4. Atlantic Ocean Red Snapper Multi-gear CRP Project 2012 (NCEI Accession 0157033)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains information useful for red snapper stock assessment. The data set provided has count, weight, length, and location available of caught red...

  5. Small-vessel Survey and Auction Sampling to Estimate Growth and Maturity of Eteline Snappers

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Small-vessel Survey and Auction Sampling to Estimate Growth and Maturity of Eteline Snappers and Improve Data-Limited Stock Assessments. This biosampling project...

  6. 78 FR 31511 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2013-05-24

    ...-economic benefits to snapper-grouper fishermen and communities that utilize the snapper-grouper resource... stock is neither overfished nor currently undergoing overfishing. The assessment results suggest the... the FMP established a suite of management measures to end the overfishing of gag (74 FR 30964, June 29...

  7. 75 FR 57734 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery off the...

    Science.gov (United States)

    2010-09-22

    ... measures (AMs) for nine snapper-grouper species, eight of which are undergoing overfishing; specify... address overfishing of eight snapper-grouper species while maintaining catch levels consistent with...-Stevens Act in 2006 require that if a stock or stock complex in an FMP is undergoing overfishing, the FMP...

  8. 76 FR 9530 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2011-02-18

    ... Assessment and Review (SEDAR) benchmark stock assessment for red snapper, SEDAR 15, which was completed in... snapper. Through the SEDAR 24 benchmark stock assessment, updated information on the status of the red... scientific information from the SEDAR 24 benchmark stock assessment. The Council voted to approve Regulatory...

  9. 75 FR 79309 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2010-12-20

    ...-Grouper Fishery Off the South Atlantic States; Emergency Rule To Delay Effectiveness of the Snapper... Atmospheric Administration (NOAA), Commerce. ACTION: Temporary rule; correction. SUMMARY: This document contains a correction to the temporary rule that delays the effective date of the area closure for snapper...

  10. Hierarchical structure and cytocompatibility of fish scales from Carassius auratus

    International Nuclear Information System (INIS)

    Fang, Zhou; Wang, Yukun; Feng, Qingling; Kienzle, Arne; Müller, Werner E.G.

    2014-01-01

    To study the structure and the cytocompatibility of fish scales from Carassius auratus, scanning electron microscopy (SEM) was used to observe the morphology of fish scales treated with different processing methods. Based on varying morphologies and components, the fish scales can be divided into three regions on the surface and three layers in vertical. The functions of these three individual layers were analyzed. SEM results show that the primary inorganic components are spherical or cubic hydroxyapatite (HA) nanoparticles. The fish scales have an ∼ 60° overlapped plywood structure of lamellas in the fibrillary plate. The plywood structure consists of co-aligned type I collagen fibers, which are parallel to the HA lamellas. X-ray diffraction (XRD), thermogravimetric analysis/differential scanning calorimetry (TGA/DSC) and Fourier transform infrared (FTIR) analysis indicate that the main components are HA and type I collagen fibers. MC3T3-E1 cell culture results show a high cytocompatibility and the ability to guide cell proliferation and migration along the scale ridge channels of the fish scales. This plywood structure provides inspiration for a structure-enhanced composite material. - Highlights: • The Carassius auratus fish scale can be divided into 3 layers rather than 2. • The functions of these three individual layers were firstly analyzed. • The fish scale shows a high cytocompatibility. • The fish scale can guide cells migration along the scale ridge channels

  11. Hierarchical structure and cytocompatibility of fish scales from Carassius auratus

    Energy Technology Data Exchange (ETDEWEB)

    Fang, Zhou [State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China); Wang, Yukun [State Key Laboratory of Geological Processes and Mineral Resources, China University of Geosciences, Beijing 100084 (China); Feng, Qingling, E-mail: biomater@mail.tsinghua.edu.cn [State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China); Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing 100084 (China); Kienzle, Arne; Müller, Werner E.G. [Institut für Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Johannes Gutenberg-Universität, Duesbergweg 6, Mainz 55099 (Germany)

    2014-10-01

    To study the structure and the cytocompatibility of fish scales from Carassius auratus, scanning electron microscopy (SEM) was used to observe the morphology of fish scales treated with different processing methods. Based on varying morphologies and components, the fish scales can be divided into three regions on the surface and three layers in vertical. The functions of these three individual layers were analyzed. SEM results show that the primary inorganic components are spherical or cubic hydroxyapatite (HA) nanoparticles. The fish scales have an ∼ 60° overlapped plywood structure of lamellas in the fibrillary plate. The plywood structure consists of co-aligned type I collagen fibers, which are parallel to the HA lamellas. X-ray diffraction (XRD), thermogravimetric analysis/differential scanning calorimetry (TGA/DSC) and Fourier transform infrared (FTIR) analysis indicate that the main components are HA and type I collagen fibers. MC3T3-E1 cell culture results show a high cytocompatibility and the ability to guide cell proliferation and migration along the scale ridge channels of the fish scales. This plywood structure provides inspiration for a structure-enhanced composite material. - Highlights: • The Carassius auratus fish scale can be divided into 3 layers rather than 2. • The functions of these three individual layers were firstly analyzed. • The fish scale shows a high cytocompatibility. • The fish scale can guide cells migration along the scale ridge channels.

  12. Bioaccumulation of heavy metals in maricultured fish, Lates calcarifer (Barramudi), Lutjanus campechanus (red snapper) and Lutjanus griseus (grey snapper).

    Science.gov (United States)

    Nasyitah Sobihah, Nasri; Ahmad Zaharin, Aris; Khairul Nizam, Mohammad; Ley Juen, Looi; Kyoung-Woong, Kim

    2018-04-01

    Mariculture fish contains a rich source of protein, but some species may bioaccumulate high levels of heavy metals, making them unsafe for consumption. This study aims to identify heavy metal concentration in Lates calcarifer (Barramudi), Lutjanus campechanus (Red snapper) and Lutjanus griseus (Grey snapper). Three species of mariculture fish, namely, L. calcarifer, L. campechanus and L. griseus were collected for analyses of heavy metals. The concentration of heavy metal (As, Cd, Cu, Cr, Fe, Pb, Mn, Ni, Se, and Zn) was determined using inductive coupled plasma mass spectrometry (ICP-MS). The distribution of heavy metals mean concentration in muscle is Zn > Fe > As > Se > Cr > Cu > Mn > Pb > Ni > Cd for L. calcarifer, Fe > Zn > Cr > As > Ni > Mn > Se > Cu > Pb > Cd for L. campechanus and Fe > Zn > Cr > Ni > Se > Cu > As > Mn > Pb > Cd for L. griseus. Among all of the species under investigation, the highest concentration of Fe was found in the muscle tissue of L. campechanus (19.985 ± 1.773 mg kg -1 ) and liver tissue of L. griseus (58.248 ± 8.736 mg kg -1 ). Meanwhile, L. calcarifer has the lowest concentration of Cd in both muscle (0.007 ± 0.004 mg kg -1 ) and liver tissue (0.027 ± 0.016 mg kg -1 ). The heavy metal concentration in muscle tissue is below the permissible limit guidelines stipulated by the Food & Agriculture Organization, 1983 and Malaysia Food Act, 1983. The concentration of heavy metals varies significantly among fish species and tissues. L. campechanus was found to have a higher ability to accumulate heavy metals as compared to the other two species (p heavy metals compared to muscle tissue (p heavy metal concentration in mariculture fish must be performed to prevent acute and chronic food intoxication. Copyright © 2018 Elsevier Ltd. All rights reserved.

  13. Effects of dietary supplementation of Lactobacillus rhamnosus or/and Lactococcus lactis on the growth, gut microbiota and immune responses of red sea bream, Pagrus major.

    Science.gov (United States)

    Dawood, Mahmoud A O; Koshio, Shunsuke; Ishikawa, Manabu; Yokoyama, Saichiro; El Basuini, Mohammed F; Hossain, Md Sakhawat; Nhu, Truong H; Dossou, Serge; Moss, Amina S

    2016-02-01

    Pagrus major fingerlings (3·29 ± 0·02 g) were fed with basal diet (control) supplemented with Lactobacillus rhamnosus (LR), Lactococcus lactis (LL), and L. rhamnosus + L. lactis (LR + LL) at 10(6) cell g(-1) feed for 56 days. Feeding a mixture of LR and LL significantly increased feed utilization (FER and PER), intestine lactic acid bacteria (LAB) count, plasma total protein, alternative complement pathway (ACP), peroxidase, and mucus secretion compared with the other groups (P red sea bream aquaculture. Copyright © 2016 Elsevier Ltd. All rights reserved.

  14. In vivo and in vitro activities of the seed extract of Piper guineense Schum. and Thonn. against skin and gill monogenean parasites of gold fish (Carassius auratus auratus).

    Science.gov (United States)

    Ekanem, A P; Wang, M; Simon, J E; Obiekezie, A I; Morah, F

    2004-10-01

    Methanol extracts of the seeds of Piper guineense (Piperaceae) were active against gold fish (Carassius auratus auratus L. Pisces Cyprinidae) monogenean parasites. The seed extract of P. guineense was administered at different concentrations (0.5-2.0 mg/L) under in vivo and in vitro conditions. There was a higher efficacy of the effects of the extracts against fish parasites under in vitro situations than under in vivo. Three major compounds (piperanine, N-isobutyl (E,E)-2,4 decadienamide and Deltaalpha,beta-dihydrowasanine) were identified from the seed extract of Piper guineense by LC-MS analysis. Copyright 2004 John Wiley & Sons, Ltd.

  15. Thyroid function and cold acclimation in the hamster, Mesocricetus auratus

    International Nuclear Information System (INIS)

    Tomasi, T.E.; Horwitz, B.A.

    1987-01-01

    Basal metabolic rate (BMR), thyroxine utilization rate (T 4 U), and triiodothyronine utilization rate (T 3 U) were measured in cold-acclimated (CA) and room temperature-acclimated (RA) male golden hamsters, Mesocricetus auratus. Hormone utilization rates were calculated via the plasma disappearance technique using 125 I-labeled hormones and measuring serum hormone levels via radioimmunoassay. BMR showed a significant 28% increase with cold acclimation. The same cold exposure also produced a 32% increase in T 4 U, and a 204% increase in T 3 U. The much greater increase in T 3 U implies that previous assessments of the relationship between cold acclimation and thyroid function may have been underestimated and that cold exposure induces both quantitative and qualitative changes in thyroid function. It is concluded that in the cold-acclimated state, T 3 U more accurately reflects thyroid function than does T 4 U. A mechanism for the cold-induced change in BMR is proposed

  16. Estudios inmunologicos en hamsters (Cricetus auratus infectados con Schistosoma mansoni

    Directory of Open Access Journals (Sweden)

    Eduardo Monge

    1986-08-01

    Full Text Available Los resultados de este trabajo muestran que el hamster (Cricetus auratus puede ser utilizado como un modelo experimental para estudios inmunológicos en la infección por Schistosoma mansoni. Los datos obtenidos, relativos a inmunidad concomitante, producción de anticuerpo letal e inmunosupresión se asemejan a los conseguidos en otros modelos experimentales ya establecidos. Estas observaciones indican que el hámster, además de ser un hospedero satisfactorio para el mantenimiento del parásito en el laboratorio, puede ser considerado como un modelo experimental alterno cuyo crecimiento y mantenimiento son relativamente simples y además es un animal de fácil manejo.

  17. Use of riverine through reef habitat systems by dog snapper ( Lutjanus jocu ) in eastern Brazil

    Science.gov (United States)

    Moura, Rodrigo L.; Francini-Filho, Ronaldo B.; Chaves, Eduardo M.; Minte-Vera, Carolina V.; Lindeman, Kenyon C.

    2011-11-01

    The early life history of Western Atlantic snappers from the Southern hemisphere is largely unknown. Habitat use of different life stages (i.e. size categories) of the dog snapper ( Lutjanus jocu) was examined across the largest South Atlantic reef-estuarine complex (Abrolhos Shelf, Brazil, 16-19° S). Visual surveys were conducted in different habitats across the shelf (estuary, inner-shelf reefs and mid-shelf reefs). Lutjanus jocu showed higher densities on inner-shelf habitats, with a clear increase in fish size across the shelf. Individuals mangrove and rocky habitats) and inner-shelf reefs (particularly shallow fore-reefs and tide pools). Individuals ranging 10-30 cm were broadly distributed, but consistently more abundant on inner-shelf reefs. Individuals between 30 and 40 cm were more common on mid-shelf reefs, while individuals >40 cm were recorded only on mid-shelf reefs. Literature data indicate that individuals ranging 70-80 cm are common on deep offshore reefs. This pattern suggests that the dog snapper performs ontogenetic cross-shelf migrations. Protecting portions of the different habitats used by the dog snapper during its post-settlement life cycle is highlighted as an important conservation and management measure.

  18. Pre-European Maori fishing at Foxton, Manawatu, New Zealand

    International Nuclear Information System (INIS)

    Davidson, J.; Leach, F.; Greig, K.; Leach, P.

    2000-01-01

    Fish remains from excavations in four areas of the Foxton archaeological site (S24/3) were analysed. The 4109 identified bones produced a Minimum Mumber of Individuals of 1040 fish from 8 families. The assemblage was dominated by New Zealand snapper (Pagrus auratus, Family Sparidae), which comprised 80% of the total MNI. Kahawai (Arripis trutta, Family Arripidae) contributed 15% and other families only minor amounts. Snapper decreased in abundance and kahawai increased from the lower to the upper layers. The Foxton catch at all periods is different from other assemblages studied from central New Zealand. This partly reflects the local marine environment, which lacks rocky shores and reefs, but we also hypothesise that it is related to warmer surface sea water conditions in Cook Strait in the early phase of the New Zealand prehistoric period. Size frequency diagrams were constructed for snapper and kahawai. It was found that the mean fork length and mean ungutted weight of snapper increased over time. Similar changes have been observed for other species in archaeological sites in New Zealand. (author). 26 refs., 6 figs., 8 tabs

  19. Carbon isotopes in otolith amino acids identify residency of juvenile snapper (Family: Lutjanidae) in coastal nurseries

    KAUST Repository

    McMahon, Kelton

    2011-08-26

    This study explored the potential for otolith geochemistry in snapper (Family: Lutjanidae) to identify residency in juvenile nursery habitats with distinctive carbon isotope values. Conventional bulk otolith and muscle stable isotope analyses (SIA) and essential amino acid (AA) SIA were conducted on snapper collected from seagrass beds, mangroves, and coral reefs in the Red Sea, Caribbean Sea, and Pacific coast of Panama. While bulk stable isotope values in otoliths showed regional differences, they failed to distinguish nursery residence on local scales. Essential AA δ13C values in otoliths, on the other hand, varied as a function of habitat type and provided a better tracer of residence in different juvenile nursery habitats than conventional bulk otolith SIA alone. A strong linear relationship was found between paired otolith and muscle essential AA δ13C values regardless of species, geographic region, or habitat type, indicating that otolith AAs recorded the same dietary information as muscle AAs. Juvenile snapper in the Red Sea sheltered in mangroves but fed in seagrass beds, while snapper from the Caribbean Sea and Pacific coast of Panama showed greater reliance on mangrove-derived carbon. Furthermore, compound-specific SIA revealed that microbially recycled detrital carbon, not water-column-based new phytoplankton carbon, was the primary carbon source supporting snapper production on coastal reefs of the Red Sea. This study presented robust tracers of juvenile nursery residence that will be crucial for reconstructing ontogenetic migration patterns of fishes among coastal wetlands and coral reefs. This information is key to determining the importance of nursery habitats to coral reef fish populations and will provide valuable scientific support for the design of networked marine-protected areas. © 2011 Springer-Verlag.

  20. Carbon isotopes in otolith amino acids identify residency of juvenile snapper (Family: Lutjanidae) in coastal nurseries

    KAUST Repository

    McMahon, Kelton; Berumen, Michael L.; Mateo, Ivan; Elsdon, Travis S.; Thorrold, Simon R.

    2011-01-01

    This study explored the potential for otolith geochemistry in snapper (Family: Lutjanidae) to identify residency in juvenile nursery habitats with distinctive carbon isotope values. Conventional bulk otolith and muscle stable isotope analyses (SIA) and essential amino acid (AA) SIA were conducted on snapper collected from seagrass beds, mangroves, and coral reefs in the Red Sea, Caribbean Sea, and Pacific coast of Panama. While bulk stable isotope values in otoliths showed regional differences, they failed to distinguish nursery residence on local scales. Essential AA δ13C values in otoliths, on the other hand, varied as a function of habitat type and provided a better tracer of residence in different juvenile nursery habitats than conventional bulk otolith SIA alone. A strong linear relationship was found between paired otolith and muscle essential AA δ13C values regardless of species, geographic region, or habitat type, indicating that otolith AAs recorded the same dietary information as muscle AAs. Juvenile snapper in the Red Sea sheltered in mangroves but fed in seagrass beds, while snapper from the Caribbean Sea and Pacific coast of Panama showed greater reliance on mangrove-derived carbon. Furthermore, compound-specific SIA revealed that microbially recycled detrital carbon, not water-column-based new phytoplankton carbon, was the primary carbon source supporting snapper production on coastal reefs of the Red Sea. This study presented robust tracers of juvenile nursery residence that will be crucial for reconstructing ontogenetic migration patterns of fishes among coastal wetlands and coral reefs. This information is key to determining the importance of nursery habitats to coral reef fish populations and will provide valuable scientific support for the design of networked marine-protected areas. © 2011 Springer-Verlag.

  1. 78 FR 44461 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery off the...

    Science.gov (United States)

    2013-07-24

    ... tournaments occur in July which would conflict with a July fishing season and would increase traffic at the... snapper would overlap with the fishing season for black sea bass and would help solve the problem of red...

  2. Marine Fisheries Initiative Program (MARFIN) Exploratory surveys for red snapper juveniles off Florida South Atlantic coast from 2012-07-31 to 2012-09-08 (NCEI Accession 0156976)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The data set contains information on locations sampled, gears used, and whether red snapper were observed. If a red snapper was caught its total length was recorded.

  3. Development of Taenia pisiformis in golden hamster (Mesocricetus auratus

    Directory of Open Access Journals (Sweden)

    Maravilla Pablo

    2011-07-01

    Full Text Available Abstract The life cycle of Taenia pisiformis includes canines as definitive hosts and rabbits as intermediate hosts. Golden hamster (Mesocricetus auratus is a rodent that has been successfully used as experimental model of Taenia solium taeniosis. In the present study we describe the course of T. pisiformis infection in experimentally infected golden hamsters. Ten females, treated with methyl-prednisolone acetate were infected with three T. pisiformis cysticerci each one excised from one rabbit. Proglottids released in faeces and adults recovered during necropsy showed that all animals were infected. Eggs obtained from the hamsters' tapeworms, were assessed for viability using trypan blue or propidium iodide stains. Afterwards, some rabbits were inoculated with eggs, necropsy was performed after seven weeks and viable cysticerci were obtained. Our results demonstrate that the experimental model of adult Taenia pisiformis in golden hamster can replace the use of canines in order to study this parasite and to provide eggs and adult tapeworms to be used in different types of experiments.

  4. Vitamin A supplementation in diets for Goldfish (Carassius auratus

    Directory of Open Access Journals (Sweden)

    Edionei Maico Fries

    2014-09-01

    Full Text Available Vitamin A is important to the proper development and functioning of the body, appearing in several metabolic functions. This experiment was conducted with aim evaluate to the vitamin A supplementation in diets for Goldfish Carassius auratus. The experimental was completely randomized with six treatments (0, 2.000, 4.000, 8.000, 16.000 and 32.000 IU of vitamin A kg-1 diet with four repetitions into 24 experimental cages, with capacity for 150 liters useful volume, made of mesh shading, installed inside a masonry circular tank with a capacity of 25 m3 of water. 192 fish with an average initial weight of 6.66±0.57 g and initial length of 7.30±0.33 cm, feed at 8h, 11h, 14h and 17h at satiety were used. Evaluated the effects on productive performance and intensity of yellow-red coloring of the skin of the fish were. The Linear Response Plateau analysis of weight gain and length total, feed conversion and daily growth rate versus vitamin A requirement estimate showed 2,624, 2,385 and 2,358, 4,381 and 2,866 IU of vitamin A per kg diet, respectively. The data indicated that this vitamin has little or no influence on the intensity of the skin color of the Goldfish.

  5. HISTOLOGICAL STUDY ON INTERNAL ORGANS DEVELOPMENT OF MANGROVE SNAPPER, Lutjanus argentimaculatus LARVAE

    Directory of Open Access Journals (Sweden)

    Ketut Mahardika

    2012-12-01

    Full Text Available Mangrove snapper, Lutjanus argentimaculatus is one of highly economic value of marine fish commodity. Culture of this species has been developed a few years ago. Basic data on biological aspect is still limited whereas those kinds of information are needed. Histological study to determine development of internal organs of the larvae of mangrove snapper was conducted. Amount of 15-20 larvae each from 1 day after DAH hatching (1 DAH up to 30 days after hatching (30 DAH were collected, fixed in Bouin’s solution for 4-6 hours and then transferred into 70% alcohol. The samples were then embedded in paraffin wax and the sections were stained with haematoxylineosin (H&E. Results showed that the eye retina of the mangrove snapper larvae well developed on 3 DAH, whereas their digestive tract was on 5 DAH. Under this condition, the larvae started feeding as showed by present of natural food tissues in the intestine. Development of digestive tract and other internal organs reached their complete form like in adult fish after 20 DAH.

  6. Reproduction of the fish Lutjanus analis (mutton snapper; Perciformes: Lutjanidae) from Northeastern Brazil.

    Science.gov (United States)

    Teixeira, Simone F; Duarte, Yalan F; Ferreira, Beatrice P

    2010-09-01

    The mutton snapper Lutjanus analis is an overexploited species of economic importance for the Northeast region of Brazil. To determine the reproductive aspects of the mutton snapper, biological data were sampled from Bahia, Pernambuco, Paraíba, Rio Grande do Norte and Ceará States, between 7 and 200m depth, from February 1997 to December 1999, performed in the Brazilian Economic Exclusive Zone Study Program (REVIZEE-NE Program). Data on total length (TL), fork length (FL), standard length (SL), total weight (TW) and gutted weight (GW) were measured. The gonads were collected and fixed in FAACC for histological analysis on maturational stage and calculation of the gonadosomatic index (GSI). A total of 135 fishes were collected; from these, 74 were females and 61 were males. Females were between 28.0 and 69.0cm (TL) and the males between 35.0 and 75.0cm (TL). Mutton snapper are gonochorists, and the spawning season, determined by the maturity stages frequency and the GSI, occurred between November and April, with peak spawning in March. The mature females' minimum length was 28.0cm fork length, smaller than the minimum maturation length previously reported for the species in Cuba. The exploitation status of the species and the biological traits enhances the importance of establishing spatial and seasonal protection measures such as protected areas both in nursery and spawning sites.

  7. Development of immune functionality in larval and juvenile crimson snapper Lutjanus erythropterus (Bloch 1790

    Directory of Open Access Journals (Sweden)

    Ke Cui

    2018-05-01

    Full Text Available Ontogenetic development of the immune system in crimson snapper (Lutjanus erythropterus Bloch 1790 larvae was histologically and enzymatically studied from hatch to 36 days post-hatch (DPH. Primitive hepatopancreas appeared on 2 DPH and renal tubules started hematopoiesis on 4 DPH. The spleen anlage appeared on 6 DPH and the thymus formed on 14 DPH. Total activities of superoxide dismutase (SOD, catalase (CAT, glutathione peroxidase (GPX and sodium-potassium adenosine triphosphatase (Na+ K+-ATPase gradually increased after hatch, and showed a sharp increase after 29 DPH during the transitional feeding period from Artemia to inert feed. The specific activities of SOD, CAT, and GPX showed a trend of sharp increase and reached the maximum level on 4 DPH when exogenous feeding started, except for Na+ K+-ATPase where the peak occurred on10 DPH. The specific activities of these five enzymes reached the peak during the food transition from rotifers to Artemia, but the total activity of enzymes showed an increasing trend as fish grew. The present study provides new knowledge of the development of functional enzymes relevant to fish larvae immunity, sheds light on the understanding of the change of larval health, and improves hatchery management of crimson snapper. Keywords: Immune system, Enzyme activity, Ontogenetic development, Crimson snapper Lutjanus erythropterus

  8. An approach to unraveling the coexistence of snappers (Lutjanidae using otolith morphology

    Directory of Open Access Journals (Sweden)

    Zahra Sadighzadeh

    2014-09-01

    Full Text Available The sagittae otolith morphology of marine fishes has been used in many ecomorphological studies to explain certain ecological adaptations of species to habitat. Our study compares the sagittal otolith shapes of ten species of snappers (Family Lutjanidae inhabiting the Persian Gulf. We used a morphometric analysis of the otolith measurements (length, height, perimeter, area and weight and of the ratio between the area of the sulcus acusticus and the area of the otolith (S:O. The otolith contour was also analysed using wavelets as a mathematical descriptor. Morphological variations in the otoliths were associated with the morphology and external colouration of snappers as well as ecological traits. An analysis of the interspecific S:O ratio suggested that the highest ratios occurred in snappers inhabiting shallower waters. A categorical multivariate analysis, including morphological, ecological and otolith size factors, showed that the species adapted to dim light conditions had a greater otolith perimeter. An analysis of variance of the otolith contour revealed zones with a higher interspecific variability, although only the antero-dorsal zone showed differing patterns. Although the otolith patterns appear to have a phylogenetic component, they might also be related to diel activity rhythms or to the light conditions in the habitat. The results of the study showed that variation in otolith morphology can be used to explain the coexistence of sympatric species.

  9. Brief communication: a cranial morphometric assessment of the taxonomic affinities of Trachypithecus auratus (E. Geoffroy, 1812 primates: Colobinae) with a reassessment of the T. auratus type specimen.

    Science.gov (United States)

    Ingicco, Thomas; Balzeau, Antoine; Callou, Cécile; Fitriana, Yuliana Sulistya

    2011-10-01

    The debate over the taxonomic position and affinities of Trachypithecus auratus has been ongoing since its identification by E. Geoffroy Saint-Hilaire in 1812. The type specimen of this species is housed in Muséum national d'Histoire naturelle in Paris (MNHN-ZM 2005-912). This point is debated due to the complex and fluctuating taxonomy of Southeast Asian Colobinae (Brandon-Jones et al.: Int J Primatol 25 (2004) 97-164) and to the fact that this individual is represented by a mounted skeleton. By means of 3D medical imaging methodologies we describe for the first time the cranial anatomy of the specimen MNHN-ZM 2005-912 and compare it with other Trachypithecus species, in order to test the molecular systematic hypotheses for affinities among the T. auratus-T. cristatus group. We ascertain the taxonomic attribution of this individual to the species Trachypithecus auratus species. The most diagnostic characters shared by the type specimen and Trachypithecus auratus compared to other species of Trachypithecus are the rounded orbits and the straight facial profile. We then try to clarify the inconsistencies concerning the geographical provenance of the type. The island of Java appears to be the most probable locality from a cluster analysis based on linear morphometry. After this approach and a discriminant analysis, a northeastern Javanese provenance of this specimen, as proposed by Brandon-Jones et al. (Int J Primatol 25 (2004) 97-164) is dubious. Finally we provide 3D models of the skull and the endocast, and a list of cranial landmark coordinates of the holotype for future research. Copyright © 2011 Wiley-Liss, Inc.

  10. Fin Type Variation of Lionhead Strain Goldfish (Carassius auratus Offspring

    Directory of Open Access Journals (Sweden)

    M. Syaifudin

    2007-12-01

    Full Text Available  Lionhead strain goldfish (Carassius auratus inheritance produce many variations in phenotype qualitative traits of their offspring that is not common with the parents. Lionhead is an ornamental freshwater fish, they do not have a dorsal fin, but it is a beauty finfish, is popular to the people and have a high economic value. Of the 846 offspring of lionhead is produced in this experiment, and 57,7% of them have dorsal fin (42,3% normal, 13,1% of them have anal fin which did not similar with their parents, 58,6% caudal fin of them did not have similar to their parents. It might be caused by incompletely segregation in meiosis and many gen responsible to certain phenotype trait. Another abnormalities such as no anal fin, blindness, stumped and no pigmen in their gill lamella also occurred. Key words: Inheritance, fin, phenotype, abnormality   ABSTRAK Ikan maskoki strain lionhead menghasilkan keturunan dengan fenotip yang sangat bervariasi dan berbeda dengan induknya. Ikan ini merupakan ikan hias air tawar yang tidak memiliki sirip punggung namun tetap memiliki keindahan, sehingga menjadi begitu populer di masyarakat dan memiliki nilai ekonomi yang tinggi. Sebanyak 846 keturunan (anakan telah dihasilkan dalam percobaan ini, dan sebanyak 57,7% dari populasi tersebut memiliki sirip punggung (berarti 42,3% merupakan keturunan normal, 13,1% memiliki sirip dubur yang tidak mirip dengan induknya. Variasi keturunan ikan maskoki strain lionhead ini disebabkan oleh segregasi yang tidak sempurna dalam proses meiosis dan banyaknya gen yang terlibat dalam pembentukan penotip tertentu. Abnormalitas lainnya juga terjadi pada keturunan ikan maskoki strain lionhead ini, seperti tidak adanya sirip dubur, mata buta, tubuh pendek dan tidak adanya pigmen pada lemela insang Kata kunci: Keturunan, sirip, fenotip, abnormalitas  

  11. Trophic and reproductive ecology of Red Snapper, Lutjanus campechanus, on natural and artificial reefs in the western Gulf of Mexico

    Science.gov (United States)

    Ajemian, M. J.; Wetz, J. J.; Brewton, R. A.; Downey, C. H.; Stunz, G. W.

    2016-02-01

    Energy exploration in the Gulf of Mexico (Gulf) has resulted in the addition of numerous oil and gas production rigs that have added structurally complex habitat to an area otherwise dominated by bare bottom. The impact of these artificial structures on fish populations is largely unknown and there is ongoing debate about their functionality. Red Snapper (Lutjanus campechanus), an ecologically and economically important sportfish to the region, use natural as well as the artificial reefs created by standing and reefed (toppled or cutoff) oil and gas rigs. However, little is known about how trophic and reproductive characteristics of Red Snapper vary over these multiple habitat types. We analyzed stable isotopic composition (δ13C, δ 15N) of epaxial muscle and compared reproductive potential of Red Snapper (155-855 mm TL) from standing rigs, reefed rigs, and natural hard-bottom habitats off Texas. Red Snapper from standing rig sites were isotopically enriched in δ 15N compared to lower relief habitats, suggesting they were feeding at a higher trophic level on standing rigs. While gonadosomatic indices (GSI) and comparative histology implied a similar spawning season among structure types, GSI was highest for both sexes at standing rigs. These initial results suggest that while standing rigs appear to provide more enriched food resources leading to higher Red Snapper reproductive capacity, the productivity of this species is similar between currently permitted rig decommissioning options (i.e., cutoff and toppled rigs) and natural hard-bottom habitats in the Gulf of Mexico.

  12. PTHrP potentiating estradiol-induced vitellogenesis in sea bream (Sparus auratus, L.)

    NARCIS (Netherlands)

    Bevelander, G.S.; Hang, X.; Abbink, W.; Spanings, F.A.T.; Canario, A.V.; Flik, G.

    2006-01-01

    In fish, vitellogenin is an important nutritional precursor protein produced solely in the liver and released into the blood where it binds calcium. In the gilthead sea bream (Sparus auratus) 17beta-Estradiol (E2) plays an important role in the synthesis of vitellogenin, but also the pituitary

  13. Carassius auratus gibelio—the most successful invasive fish in waters of the Czech Republic

    Czech Academy of Sciences Publication Activity Database

    Lusková, Věra; Lusk, Stanislav; Halačka, Karel; Vetešník, Lukáš

    2010-01-01

    Roč. 1, č. 3 (2010), s. 176-180 ISSN 2075-1117 R&D Projects: GA MŽP SP/2D4/55/07 Institutional research plan: CEZ:AV0Z60930519 Keywords : invasive alien fish * Carassius auratus gibelio * sex ratio * ploidy status * impact Subject RIV: EH - Ecology, Behaviour

  14. The involvement of thyroid hormone metabolism in gilthead sea bream (Sparus auratus) osmoregulation

    NARCIS (Netherlands)

    Klaren, P.H.M.; Guzman, J.M.; Mancera, J.M.; Geven, E.J.W.; Flik, G.

    2005-01-01

    We have investigated the effect of adaptation to low salinity water on the thyroid status of the euryhaline teleost, Sparus auratus. We show that, following low salinity adaptation, the plasma T(4) concentration increases and branchial deiodination activities of T(4), T(3), and rT(3) decrease.

  15. Biogeography and evolution of the Carassius auratus-complex in East Asia

    Directory of Open Access Journals (Sweden)

    Iguchi Kei'ichiro

    2010-01-01

    Full Text Available Abstract Background Carassius auratus is a primary freshwater fish with bisexual diploid and unisexual gynogenetic triploid lineages. It is distributed widely in Eurasia and is especially common in East Asia. Although several genetic studies have been conducted on C. auratus, they have not provided clear phylogenetic and evolutionary descriptions of this fish, probably due to selection bias in sampling sites and the DNA regions analysed. As the first step in clarifying the evolutionary entity of the world's Carassius fishes, we attempted to clarify the phylogeny of C. auratus populations distributed in East Asia. Results We conducted a detailed analysis of a large dataset of mitochondrial gene sequences [CR, 323 bp, 672 sequences (528 sequenced + 144 downloaded; CR + ND4 + ND5 + cyt b, 4669 bp in total, 53 sequences] obtained from C. auratus in East Asia. Our phylogeographic analysis revealed two superlineages, one distributed mainly among the Japanese main islands and the other in various regions in and around the Eurasian continent, including the Ryukyus and Taiwan. The two superlineages include seven lineages with high regional specificity that are composed of endemic populations indigenous to each region. The divergence time of the seven lineages was estimated to be 0.2 million years ago (Mya by a fossil-based method and 1.0-1.9 Mya by the molecular clock method. The antiquity and endemism of these lineages suggest that they are native to their respective regions, although some seem to have been affected by the artificial introduction of C. auratus belonging to other lineages. Triploids of C. auratus did not form a monophyletic lineage but were clustered mostly with sympatric diploids. Conclusions The results of the present study revealed the existence of two superlineages of C. auratus in East Asia that include seven lineages endemic to each of the seven regions examined. The lack of substantial genetic separation between triploids and

  16. Action of neuro-hypophysis hormones on sodium exchanges of Carassius auratus L; Action des hormones neurohypophysaires sur les echanges de sodium de Carassius auratus L.

    Energy Technology Data Exchange (ETDEWEB)

    Julien, Monique

    1960-06-25

    This academic work reports the use of radio-sodium as indicator of sodium exchanges to simultaneously measure the gill input flow and the output gill and urinary flows. This technique has been applied in the case of a common soft water fish (Carassius auratus L.) to study the possible action of neuro-hypophysis extracts on these flows, and the action of these hormones on urinary excretion [French] Nous avons mesure les echanges de sodium de Carassius auratus a l'aide de radiosodium 24. L'action d'extraits neuro-hypophysaires d'origine diverse a ete comparee a celle d'injection temoin de chlorure de sodium isotonique, aussi bien sur les flux d'entree et de sortie de sodium que sur l'excretion urinaire. De ces experiences faites in vivo, nous pouvons tirer les conclusions suivantes: 1 - L'extrait neurohypophysaire de Carassius auratus provoque une augmentation du flux d'entree de sodium au niveau de la branchie de cet animal alors que le flux de sortie reste inchange. Cet extrait semble plus actif qu'une dose d'ocytocine egale a son activite ocytocique propre. Parmi les hormones neurohypophysaires de mammiferes, seule l'ocytocine (a forte dose) produit une action analogue a celle decrite ci-dessus. L'extrait de Rana esculenta reste sans action. Nous en avons conclu a l'existence dans la neurohypophyse de Carassius auratus d'un facteur hormonal agissant au niveau de la branchie pour controler l'osmoregulation de ce poisson. 2 - Les extraits neurohypophysaires de Carassius auratus entrainent chez l'animal une augmentation de la diurese et de la concentration de sodium de l'urine. Cependant cette action reste discrete et fugace. Les hormones de mammiferes, et les extraits neurohypophysaires d'amphibiens restent sans action. 3 - D'autres auteurs ont montre que chez les amphibiens, l'action des hormones neurohypophysaires se traduit a la fois par une augmentation du transport actif de sodium a travers la peau et par un desequilibre de la balance hydrique (augmentation du

  17. Antidiabetic Effects of Carassius auratus Complex Formula in High Fat Diet Combined Streptozotocin-Induced Diabetic Mice

    Directory of Open Access Journals (Sweden)

    Zhi-Hong Wang

    2014-01-01

    Full Text Available Carassius auratus complex formula, including Carassius auratus, Rhizoma dioscoreae, Lycium chinense, and Rehmannia glutinosa Libosch, is a combination prescription of traditional Chinese medicine, which has always been used to treat diabetes mellitus in ancient China. In this study, we provided experimental evidence for the use of Carassius auratus complex formula in the treatment of high fat diet combined streptozotocin- (STZ- induced type 2 diabetes. Carassius auratus complex formula aqueous extract was prepared and the effects of it on blood glucose, serum insulin, adipose tissue weight, oral glucose tolerance test (OGTT, total cholesterol, and triglyceride (TG levels in mice were measured. Moreover, adiponectin, TG synthesis related gene expressions, and the inhibitory effect of aldose reductase (AR were performed to evaluate its antidiabetic effects. After the 8-week treatment, blood glucose, insulin levels, and adipose tissue weight were significantly decreased. OGTT and HOMA-IR index showed improved glucose tolerance. It could also lower plasma TG, TC, and liver TG levels. Furthermore, Carassius auratus complex formula could inhibit the activity of AR and restore adiponectin expression in serum. Based on these findings, it is suggested that Carassius auratus complex formula possesses potent anti-diabetic effects on high fat diet combined STZ-induced diabetic mice.

  18. The effect of finasteride on spermatogenesis of Mesocricetus auratus Impacto da finasterida na espermatogênese do Mesocricetus auratus

    Directory of Open Access Journals (Sweden)

    Dimas José Araújo Vidigal

    2008-06-01

    Full Text Available PURPOSE: To study the effect of finasteride on the spermatogenesis of adult Mesocricetus auratus. METHODS: Twenty adult hamsters were evaluated. The animals were one year-older, and were randomly divided in 2 different groups: control group with ten animals (n=10 and experimental group also with ten animals (n=10. The animals in the experimental group were shot 7.14 ng/mL (0.5mL of finasteride by 100mg/Kg, subcutaneously in the dorsal region three times per week during 90 days. This dose correspondes to 5mg of the drug used in adult men for the treatment of benign prostatic hyperplasia (BPH. After three months, the animals were anesthetized through association of 200mg/kg ketamine chloridrate and 2.5 mg/kg of diazepan and were dead through hypovolemia.. The testis removed along with the whole genitourinary apparel were fixed with 10% formalin and submitted to histological analisys by optical microscopy. The hematoxilin-eosin (HE method was used to stain the slides. RESULTS: The mean weight of animals in the control group before death was 129.0±18.8gr. The mean weight of animals in experimental group was 145.0±15.25gr. The mean age of animals in control group before death was 15.2±1.13 months. The mean age of animals in experimental group before death was 17.16±0.82 months. The mean difference in weight between both groups was not statistical significant (p=0.0514. The totality of animals in control group (100% presented no tubular alterations and showed no disturbancy in the spermatogenesis stages. Four animals (40% in the experimental group showed hypotrophy of the seminiferous tubules and six (60% showed normal spermatogenesis, however reduced compared to control group. There was statiscally significant difference (p=0.043 between the control and experimental group related to testicular alterations. CONCLUSION: The animals that were administered finasteride showed significant tubules atrophy and spermatogenesis reduction compared to

  19. Spatial variation in density and size structure indicate habitat selection throughout life stages of two Southwestern Atlantic snappers.

    Science.gov (United States)

    Aschenbrenner, Alexandre; Hackradt, Carlos Werner; Ferreira, Beatrice Padovani

    2016-02-01

    The early life history of Lutjanus alexandrei and Lutjanus jocu in Southwestern Atlantic is still largely unknown. Habitat use of different life stages (i.e. size categories and densities) of the Brazilian snapper (L. alexandrei) and dog snapper (L. jocu) was examined in a tropical portion of NE coast of Brazil. Visual surveys were conducted in different shallow habitats (mangroves and reefs). Both snapper species showed higher densities in early life stages in mangrove habitat, with a clear increase in fish size from mangrove to adjacent reefs. Post-settler individuals were exclusively found in mangroves for both species. Juveniles of L. alexandrei were also registered only in mangroves, while sub-adult individuals were associated with both mangrove and reef habitats. Mature individuals of L. alexandrei were only observed in reef habitats. Juvenile and sub-adult individuals of the dog snapper were both associated with mangrove and reef habitats, with high densities registered in mangroves. Mature individuals of L. jocu were not registered in the study area. This pattern suggests preference for mangrove habitat in early life stages for both species. Ontogenetic movement between habitats was also recorded. This pattern denotes habitat selection across different life cycle of both species. Such information highlights the importance of directing management and conservation efforts to these habitats to secure the continuity of contribution to adult populations. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. 78 FR 59635 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2013-09-27

    ... Magnuson-Stevens Act requires fishery managers to end overfishing of stocks and to minimize bycatch and..., which would lower overall cost, or as a marketing tool to attract additional angler trips, which could... illegal, this practice could pose problems in tracking recreational versus commercial landings of snapper...

  1. 78 FR 39700 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery off the...

    Science.gov (United States)

    2013-07-02

    ... this rule is to provide socio-economic benefits to snapper-grouper fishermen and communities that... councils to prevent overfishing and to achieve, on a continuing basis, the OY for federally managed fish... fishery managers to end overfishing of stocks while achieving OY from the fishery, and to minimize bycatch...

  2. Francisella marina sp. nov., etiologic agent of systemic disease in cultured spotted rose snapper (Lutjanus guttatus) in Central America

    Science.gov (United States)

    Historically, piscine francisellosis in various warm, temperate and coldwater fish hosts has been attributed to Francisella noatunensis. From 2015-2016, an undescribed Francisella sp. was recovered during mortality events in cultured spotted rose snapper (Lutjanus guttatus) off the Pacific coast of ...

  3. 78 FR 78770 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2013-12-27

    ... a marketing tool to attract additional angler trips, which could bring in additional revenues. It is... targeting mainly Spanish or king mackerel is different from that with vessels mainly dependent on snapper... blue runner from the FMP. These vessels may take as many trips targeting blue runner as they can...

  4. 77 FR 1908 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2012-01-12

    .... SUMMARY: NMFS announces that the South Atlantic Fishery Management Council (Council) has submitted Amendment 20A to the Fishery Management Plan (FMP) for the Snapper-Grouper Fishery of the South Atlantic... for the wreckfish individual transferable quota (ITQ) program, including actions to define and revert...

  5. 78 FR 46923 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery off the...

    Science.gov (United States)

    2013-08-02

    ... the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery off the South Atlantic... following an ACL overage, could have adverse economic and social impacts on fishery participants... implementing the procedural provisions of the National Environmental Policy Act (NEPA; 40 CFR parts 1500-1508...

  6. 77 FR 50672 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2012-08-22

    ... National Environmental Policy Act, including biological, economic, social, administrative, and cumulative... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration 50 CFR Part 622 RIN 0648-BC30 Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

  7. 78 FR 11628 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery of the...

    Science.gov (United States)

    2013-02-19

    ... collected from the fish for genetic analysis, and age and growth studies. Additional information on the... Texas A&M University in College Station, TX, for genetic analysis. Otoliths would also be removed from... Fishery Management Council (Council) and NMFS data that may be used for future management of red snapper...

  8. 75 FR 5950 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper and Grouper Off the...

    Science.gov (United States)

    2010-02-05

    ... States AGENCY: National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric Administration... United States. This would include reef fish, red drum, coastal migratory pelagics, stone crab, and lobsters in the Gulf of Mexico, and snapper-grouper, coastal migratory pelagics, dolphin and wahoo, and...

  9. 75 FR 62488 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery off the...

    Science.gov (United States)

    2010-10-12

    ... U.S.C. 1801 et seq. 2. In Sec. 622.2, the definitions of ``Deep-water grouper (DWG)'' and ``Shallow... neither overfished nor undergoing overfishing. Red snapper overfishing is being addressed separately in... and possession of all South Atlantic shallow-water groupers (gag, black grouper, red grouper, scamp...

  10. Effects of Large-Scale Releases on the Genetic Structure of Red Sea Bream (Pagrus major, Temminck et Schlegel) Populations in Japan.

    Science.gov (United States)

    Blanco Gonzalez, Enrique; Aritaki, Masato; Knutsen, Halvor; Taniguchi, Nobuhiko

    2015-01-01

    Large-scale hatchery releases are carried out for many marine fish species worldwide; nevertheless, the long-term effects of this practice on the genetic structure of natural populations remains unclear. The lack of knowledge is especially evident when independent stock enhancement programs are conducted simultaneously on the same species at different geographical locations, as occurs with red sea bream (Pagrus major, Temminck et Schlegel) in Japan. In this study, we examined the putative effects of intensive offspring releases on the genetic structure of red sea bream populations along the Japanese archipelago by genotyping 848 fish at fifteen microsatellite loci. Our results suggests weak but consistent patterns of genetic divergence (F(ST) = 0.002, p Red sea bream in Japan appeared spatially structured with several patches of distinct allelic composition, which corresponded to areas receiving an important influx of fish of hatchery origin, either released intentionally or from unintentional escapees from aquaculture operations. In addition to impacts upon local populations inhabiting semi-enclosed embayments, large-scale releases (either intentionally or from unintentional escapes) appeared also to have perturbed genetic structure in open areas. Hence, results of the present study suggest that independent large-scale marine stock enhancement programs conducted simultaneously on one species at different geographical locations may compromise native genetic structure and lead to patchy patterns in population genetic structure.

  11. Action of neuro-hypophysis hormones on sodium exchanges of Carassius auratus L

    International Nuclear Information System (INIS)

    Julien, Monique

    1960-01-01

    This academic work reports the use of radio-sodium as indicator of sodium exchanges to simultaneously measure the gill input flow and the output gill and urinary flows. This technique has been applied in the case of a common soft water fish (Carassius auratus L.) to study the possible action of neuro-hypophysis extracts on these flows, and the action of these hormones on urinary excretion [fr

  12. Erythrocyte profile of diploid and triploid silver crucian carp (Carassius auratus)

    Czech Academy of Sciences Publication Activity Database

    Vetešník, Lukáš; Halačka, Karel; Lusková, Věra; Lusk, Stanislav

    2006-01-01

    Roč. 75, č. 2 (2006), s. 203-207 ISSN 0001-7213 R&D Projects: GA ČR GA206/05/2159 Institutional research plan: CEZ:AV0Z60930519 Keywords : Carassius auratus * complete red blood count * erythrocyte nuclear dimensions Subject RIV: EG - Zoology Impact factor: 0.491, year: 2006 http://vfu-www.vfu.cz/acta-vet/vol75/75-203.pdf

  13. Tingkah Laku Makan Lutung Jawa Trachypithecus Auratus Di Kawasan Pancuran 7 Baturaden Gunung Slamet Jawa Tengah

    OpenAIRE

    Eliana, Dwi; Nasution, Erie Kolya; Indarmawan, Indarmawan

    2017-01-01

    Javan Langur (Trachypithecus auratus) has an important ecological role in conserving the forest as their habitat by spreading seeds of fruits of their food. They are also responsible for the pattern of the plant diversity and forest regeneration as their habitat. Unfortunately, Javan Langur has been listed on Appendix II of CITES and categorized as vulnerable by the IUCN. Pancuran 7 Baturaden on the Mount Slamet slope is one of the few Javan Langur habitats in Java. Information on the Javan L...

  14. Bioceramics synthesis of hydroxyapatite from red snapper fish scales biowaste using wet chemical precipitation route

    Science.gov (United States)

    Ulfyana, D.; Anugroho, F.; Sumarlan, S. H.; Wibisono, Y.

    2018-03-01

    Fish scales biowaste contain high collagens and calcium phosphates, therefore have considerable potential as raw material for value-added biomaterial such as hydroxyapatite (HAp). HAp is the main constituent component of hard tissue such as bone and teeth in the human body and is known as bioceramic materials. In this work, wet chemical precipitation method was used to syntesize HAp from Red Snapper Fish (Lutjanus campechanus) Scales. Two variations of calcination temperatures of 600°C (FHAp1) and 800°C (FHAp2) were conducted for 5 hours. The results showed calcium content from biowaste of red snapper fish scale was 83.62%. FTIR result shows that PO4 3-, OH-, and CO3 2- functional groups presence as indicates the formation of HAp. XRD result showed the degree of crystallinity for FHAp1 and FHAp2 were 75.52% and 79.20%, respectively. The degree of crystallinity is in accordance with ISO 13779-2:2000 standard in which the minimum degree of crystallinity of hydroxyapatite used for biomedical materials is 45%. Finally, Particle Size Analyzer (PSA) results show that the particle size distribution is evenly distributed, with the size of micro-scale hydroxyapatite particles, ranging from 5.76 μm to 132.64 μm.

  15. Tempo and mode of recurrent polyploidization in the Carassius auratus species complex (Cypriniformes, Cyprinidae).

    Science.gov (United States)

    Luo, J; Gao, Y; Ma, W; Bi, X-y; Wang, S-y; Wang, J; Wang, Y-q; Chai, J; Du, R; Wu, S-f; Meyer, A; Zan, R-g; Xiao, H; Murphy, R W; Zhang, Y-p

    2014-04-01

    Polyploidization is an evolutionarily rare but important mechanism in both plants and animals because it increases genetic diversity. Goldfish of the Carassius auratus species complex can be tetraploids, hexaploids and octaploids. Polyploidization events have occurred repeatedly in goldfish, yet the extent of this phenomenon and its phyletic history are poorly understood. We explore the origin, tempo and frequency of polyploidization in Chinese and Japanese goldfish using both mitochondrial (mtDNA) and nuclear DNA sequences from up to 1202 individuals including the outgroup taxon, Cyprinus carpio. Analyses of de novo nuclear gene data resolve two clusters of alleles and the pattern supports the prior hypothesis of an ancient allotetraploidization for Carassius. Alleles shared by tetraploid and hexaploid individuals indicate recent autoploidizations within the C. auratus complex. Sympatric tetraploids and hexaploids share mtDNA haplotypes and these frequently occur independently within six well-supported lineages and sublineages on a small spatial scale. Gene flow estimates (Fst values) indicate that hexaploids differ only slightly from sympatric tetraploids, if at all. In contrast, allopatric populations of tetraploids and hexaploids differ from one another to a far greater extent. Gene flow between sampled localities appears to be limited. Coalescence-based time estimations for hexaploids reveal that the oldest lineage within any sampled locality is around one million years old, which is very young. Sympatric, recurrent autoploidization occurs in all sampled populations of the C. auratus complex. Goldfish experience polyploidization events more frequently than any other vertebrate.

  16. Radioecological studies of [sup 137]Cs in limnological ecosystems. Accumulation and excretion of [sup 137]Cs in goldfish, Carassium auratus auratus, rearing in the radioactive freshwater

    Energy Technology Data Exchange (ETDEWEB)

    Miyake, Sadaaki; Motegi, Misako; Oosawa, Takashi; Nakazawa, Kiyoaki (Saitama Institute of Public Health, Urawa (Japan)); Ogata, Hiromitsu; Izumo, Yoshiro; Nakamura, Fumio

    1994-11-01

    Accumulation and excretion of [sup 137]Cs in goldfish, Carassius auratus auratus, rearing in the radioactive freshwater were investigated in order to elucidate the accumulation mechanism of the radionuclide in natural living fishes. The accumulation of [sup 137]Cs, expressed in concentration ratio (CR), in whole body of the fish showed a rapid increasing tendency during the rearing, and the CR value reached nearly 1 at 7th day. On the other hand, the excretion of [sup 137]Cs, expressed in retention rate, in whole body rearing in non-radioactive freshwater following the accumulation above for 7 days demonstrated a gradual decreasing tendency, and the retention rate resulted in nearly 71% at 25th day. As for the tissues and organs, higher accumulation and higher excretion were found in the viscera and the gill than those in the muscle. It is so suggested that the metabolic turnover rate of this radionuclide for the viscera and the gill is more rapid than that for the muscle. (author).

  17. Artificial Reefs as Surrogate Habitats for Red Snapper in the Northwestern Gulf of Mexico: A Fishery-Independent Comparison of Artificial and Natural Habitats

    Science.gov (United States)

    Streich, M.; Wetz, J. J.; Ajemian, M. J.; Stunz, G. W.

    2016-02-01

    The goal of our study was to evaluate the relative abundance, size and age structure of Red Snapper among three different habitat types (standing oil and gas platforms, artificial reefs [rigs-to-reefs], and natural banks) in the northwestern Gulf of Mexico. From May 2013 - January 2015, we conducted 140 vertical line sets and captured 1538 Red Snapper ranging in size from 251 to 855 mm TL. Ages determined for 801 of these fish ranged from 2-30 years. No differences were detected in Red Snapper CPUE among the three habitats. However, a comparison of TL and TW distributions suggested that natural banks supported a greater proportion of larger fish than artificial reefs or standing platforms (K-S test, pdata will help elucidate the role artificial structures play in maintaining the Red Snapper population.

  18. Toxicity assessment of simulated urban runoff containing polycyclic musks and cadmium in Carassius auratus using oxidative stress biomarkers

    International Nuclear Information System (INIS)

    Chen Fang; Gao Jie; Zhou Qixing

    2012-01-01

    The objective of this study was to assess potential toxic effects of simulated urban runoff on Carassius auratus using oxidative stress biomarkers. The activity of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and the content of malondialdehyde (MDA) in the liver of C. auratus were analyzed after a 7-, 14- and 21-day exposure to simulated urban runoff containing galaxolide (HHCB) and cadmium (Cd). The results showed that the activity of antioxidant enzymes and the content of MDA increased significantly exposed to the simulated urban runoff containing HHCB alone or mixture of HHCB and Cd. The activity of the investigated enzymes and the content of MDA then returned to the blank level over a longer period of exposure. The oxidative stress could be obviously caused in the liver of C. auratus under the experimental conditions. This could provide useful information for toxic risk assessment of urban runoff. - Highlights: ► We assessed potential toxicity of urban runoff containing HHCB and Cd. ► Exposure of simulated urban runoff can caused oxidative stress in C. auratus liver. ► SOD and CAT are more sensitive than POD and more suitable for indicating the toxicity of urban runoff. ► The present study using oxidative stress biomarkers could provide useful information for toxic risk assessment of urban runoff. - Simulated urban runoff containing HHCB and Cd could cause oxidative stress on the liver of Carassius auratus, which could provide useful information for toxic risk assessment of urban runoff.

  19. Interspecies differences in the accumulation of tributyltin and its metabolites under dietary exposure in sea perch, Lateolabrax japonicus, and red sea bream, Pagrus major.

    Science.gov (United States)

    Uno, Seiichi; Murakami, Masayo; Kokushi, Emiko; Koyama, Jiro

    2011-02-01

    Interspecies differences in the accumulation of dietary tributyltin (TBT) between sea perch, Lateolabrax japonicus, and red sea bream, Pagrus major, were studied. Although TBTs in both species reached steady-state condition in low-concentration group (L-group, 200 ng/g nominal concentration) by 1 week, it increased up to the end of exposure in high-concentration group (H-group, 3000 ng/g nominal concentration). In H-group, the accumulation rate of TBT in sea perch from 1 to 2 weeks was much higher than in red sea bream, which were 2.4-fold for sea perch and 1.7-fold for red sea bream, although TBT concentrations were similar between sea perch and red sea bream in L-group. Furthermore, in the H-group, the concentrations of TBT at 1 and 2 weeks were about 1.3- and 1.9-fold, respectively, higher in the sea perch than in the red sea bream. On the other hand, DBT residue in red sea bream was about 1.4-fold higher in sea perch for the L-group but concentrations were similar in both fishes for the H-group. These results suggest that red sea bream could metabolize faster the TBT to DBT than sea perch. This study also reveals that fish probably could absorb TBT through the food chain. The uptake of TBT by fish should be regarded in the real environment, because TBT concentration in seawater has been decreasing and now already at significantly low level. Copyright © 2009 Wiley Periodicals, Inc.

  20. Effects of dietary administration of guanosine monophosphate on the growth, digestibility, innate immune responses and stress resistance of juvenile red sea bream, Pagrus major.

    Science.gov (United States)

    Hossain, Md Sakhawat; Koshio, Shunsuke; Ishikawa, Manabu; Yokoyama, Saichiro; Sony, Nadia Mahjabin

    2016-10-01

    The present study explored the dietary administration effects of guanosine monophosphate (GMP) on growth, digestibility, innate immune responses and stress resistance of juvenile red sea bream, Pagrus major. A semi-purified basal diet supplemented with 0% (Control), 0.1% (GMP-0.1), 0.2% (GMP-0.2), 0.4% (GMP-0.4) and 0.8% (GMP-0.8) purified GMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (mean initial weight 3.4 g) for 56 days. The obtained results clearly indicated that, growth performance of red sea bream enhanced by dietary GMP supplementation compared to control and significantly higher final weight was found in fish fed diet group GMP-0.4. Specific growth rate (SGR) and percent weight gain (%WG) also significantly higher in diet group GMP-0.4 in compared to control and it was not differed (P > 0.05) with diet group GMP-0.8. Feed intake significantly increased with the supplementation of GMP. Feed conversion efficiency (FCE) and protein efficiency ratio (PER) also improved (P  0.05) by GMP supplementation. In contrast, catalase activity decreased with GMP supplementation. In terms of oxidative stress GMP-0.2 showed best condition with low oxidative stress and high antioxidant level. Moreover, the fish fed GMP supplemented diets had better improvement (P red sea bream, which is also in line with the most of the growth performance and health parameters of the fish. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Dietary taurine supplementation ameliorates the lethal effect of phenanthrene but not the bioaccumulation in a marine teleost, red sea bream, Pagrus major.

    Science.gov (United States)

    Hano, Takeshi; Ito, Mana; Ito, Katsutoshi; Kono, Kumiko; Ohkubo, Nobuyuki

    2017-03-01

    The present study was performed to evaluate the effect of dietary taurine on the hepatic metabolic profiles of red sea bream (Pagrus major) and on phenanthrene (a polyaromatic hydrocarbon) toxicity and bioaccumulation. The fish were fed a diet supplemented with 0% (TAU0%), 0.5% (TAU0.5%), or 5% (TAU5%) taurine for 40-55d and subjected to phenanthrene acute toxicity and bioaccumulation tests. Taurine deficiency in feed severely affected the hepatic metabolic profiles of fish, which indicated a complementary physiological response to taurine deficiency. For the acute toxicity test, fish were fed the test diets for 55d and were then exposed to 0-893µg/L phenanthrene for 96h. Tolerance to phenanthrene was significantly improved by 0.5% of taurine inclusion in feed relative to TAU0%, but not by 5.0% inclusion. Reduced glutathione in the liver, which acts as an oxygen-free radical scavenger, was associated with a reduction in the toxicity of phenanthrene. For the bioaccumulation test, fish were fed the test diets for 40d and were thereafter chronically exposed to 20µg/L phenanthrene for 13d followed by depuration for 3d. The activity of hepatic biomarker, ethoxyresorufin-O-deethylase, was increased by phenanthrene exposure in the taurine inclusion groups. However, phenanthrene concentrations in the liver and muscle of fish fed TAU5.0% tended to be higher than those of fish fed TAU0% and TAU0.5% during the exposure period. These results indicate that 0.5% of taurine inclusion in feed plays an important role in the alleviation of phenanthrene toxicity but not bioaccumulation. Furthermore, larger amount of taurine inclusion (TAU5%) did not show marked beneficial effects against phenanthrene exposure. This study provides insight about a major concern of environmental contaminants into aquatic environment and can be effectively used for improvement of aquaculture. Copyright © 2016 Elsevier Inc. All rights reserved.

  2. Effect of taurine supplementation on hepatic metabolism and alleviation of cadmium toxicity and bioaccumulation in a marine teleost, red sea bream, Pagrus major.

    Science.gov (United States)

    Hano, Takeshi; Ito, Katsutoshi; Kono, Kumiko; Ito, Mana; Ohkubo, Nobuyuki; Mochida, Kazuhiko

    2017-02-01

    This study was performed to unravel the mechanism of the beneficial action of taurine on marine teleost fish, red sea bream (Pagrus major), by analyzing the hepatic metabolism. Moreover, the ameliorative effects of the nutrient against cadmium toxicity and bioaccumulation were further evaluated. The fish were fed a diet containing 0 % (TAU0 %), 0.5 % (TAU0.5 %), or 5.0 % (TAU5.0 %) taurine for 40-55 days (d) and subjected to cadmium acute toxicity and bioaccumulation tests. Taurine deficiency in feed severely affected growth and the hepatic metabolic profiles of the fish, including a remarkable increase in myo-inositol, aspartate, and ß-alanine in the TAU0 % group, which indicates a complementary physiological response to taurine deficiency. For the acute toxicity test, fish were fed the test diets for 55 d and were then exposed to different dose of cadmium ranging from 0 to 5.6 mg/L for 96 h. Fish fed taurine had a higher tolerance to cadmium than those not fed taurine. For the bioaccumulation test, fish were fed the test diets for 40 d and then were chronically exposed to 0.2 mg/L of cadmium for 28 d followed by depuration for 21 d. Cadmium concentrations in the liver and muscle of fish fed TAU5.0 % were significantly lower than those of fish fed TAU0 % for the first 7 d of exposure and the first 7 d of elimination. Our findings suggest a possible mechanism for the beneficial role played by taurine and that the inclusion of taurine in fish aquaculture feed may reduce cadmium contamination of fish intended for human consumption.

  3. Egg quality traits and predictors of embryo and fry viability in red snapper Lutjanus campechanus

    Directory of Open Access Journals (Sweden)

    Agnès Bardon-Albaret

    2017-08-01

    Full Text Available The quality of red snapper eggs is highly variable and unpredictable in aquaculture, leading to high mortality during early larval rearing. In this work, the viability of red snapper eggs was monitored from fertilization until unfed larvae expired because of exhaustion of vitelline reserves to determine egg quality traits in this species. The spawns were obtained via strip spawning wild-caught females following hormonal induction with chorionic gonadotropin. Females were induced immediately after capture (wild group, n = 17 or held captive for the entire maturation period prior to induction (captive group, n = 7. Candidate predictors of egg quality measured on the female parent at the time of induction or on the spawn at ovulation were evaluated using correlation and multiple regression analysis. The fertilization rate, the hatching rate, and the duration of survival of unfed larvae post hatch were weakly correlated to each other (-0.23 < r < −0.08, revealing occurrence of distinct and independent components of egg quality. Spawns from captive females were characterized by a longer latency interval between hormonal induction and ovulation, lower fecundity, and lower hatching rates, as compared to those from wild females. Among the wild brood fish, a positive correlation was observed between the age of the female and the hatching rate. The best model optimized during stepwise multiple regression analysis of hatching rate data only explained 34% of the variance for this trait and no model could be optimized for the prediction of fertilization rate or the duration of survival post hatch. These results highlight the need to develop alternative egg quality measures to predict the viability of fry with confidence.

  4. Candidatus Renichlamydia lutjani, a Gram-negative bacterium in internal organs of blue striped snapper Lutjanus kasmira from Hawaii

    Science.gov (United States)

    Corsaro, Daniele; Work, Thierry M.

    2012-01-01

    The blue-striped snapper Lutjanus kasmira (Perciformes, Lutjanidae) are cosmopolitan in the Indo-Pacific but were introduced into Oahu, Hawaii, USA, in the 1950s and have since colonized most of the archipelago. Studies of microparasites in blue-striped snappers from Hawaii revealed chlamydia-like organisms (CLO) infecting the spleen and kidney, characterized by intracellular basophilic granular inclusions containing Gram-negative and Gimenez-positive bacteria similar in appearance to epitheliocysts when seen under light microscopy. We provide molecular evidence that CLO are a new member of Chlamydiae, i.e. Candidatus Renichlamydia lutjani, that represents the first reported case of chlamydial infection in organs other than the gill in fishes.

  5. Annatto in diets Carassius auratus goldfish fingerlings: growth performance and skin pigmentation

    Directory of Open Access Journals (Sweden)

    Edionei Maico Fries

    2014-12-01

    Full Text Available This study evaluated the effect of adding annatto as agent pigmentation on the staining characteristics of the skin, muscle and productive performance of Carassius auratus, reared in 0.15 m3 (dimensions 0.50 x 0.50 x 0.65 m hapas disposed within a circular masonry tank of 25 m3. We used 336 C. auratus fingerlings completely randomized design in 28 hapas with seven treatments and four replicates with 12 fish per unit. The addition of annatto levels were 0.0, 0.25, 0.50, 1.00, 2.00, 4.00 and 8.00%. Fish with an average initial weight of 1.12 ± 0.18 g and length of 4.31 ± 0.44 cm, were fed the 08, 11, 14 and 17 hours. The b * colorimetric characteristics (yellow showed significant at 96 and 141 days in the Hunter coordinate system with the addition of 2.0 and 1.0% annatto in the diet, respectively, and the coordinate system of CMYK and 96 days for C (cyan, and 141 days for the Y (yellow, with the addition of 8.0 to 2.0% annatto in the diet, respectively. We observed differences (P <0.05 average final weight, weight gain, feed conversion, daily growth rate and feed efficiency. It is recommended a diet containing 1.0% added annatto to C. auratus to better performance and 1.0 to 4.0% added annatto to intensified light fish skin.

  6. Radioecological studies of {sup 137}Cs in limnological ecosystems. Accumulation and excretion of {sup 137}Cs in goldfish, Carassius auratus auratus, from diets

    Energy Technology Data Exchange (ETDEWEB)

    Miyake, Sadaaki; Motegi, Misako; Oosawa, Takashi; Nakazawa, Kiyoaki [Saitama Inst. of Public Health, Urawa (Japan); Ogata, Hiromitsu; Izumo, Yoshiro; Nakamura, Fumio

    1996-06-01

    Accumulation and excretion Of {sup 137}Cs in goldfish, Carassius auratus auratus, from radioactive diets (killifish, Oryzias latipes, meats accumulated {sup 137}Cs in the radioactive freshwater) or control diets (killifish meats mixed {sup 137}CsCl) were investigated in order to elucidate the accumulation mechanism of the radionuclide in natural living fishes. The accumulation of {sup 137}Cs, expressed in concentration ratio, in whole body of the fish showed a increasing tendency in proportion to the rearing time. On the other hand, the excretion of {sup 137}Cs, expressed in retention rate, in whole body rearing by administration of non-radioactive commercial diets after the accumulation above for 7 days, demonstrated a rapid decreasing at first few days, and followed a gradual decreasing tendency (the biological half lives were about 1.5 days and 52 days, respectively). As for the tissues and organs, higher accumulation and higher excretion were found in the viscera than those in the muscle. It is so suggested that the metabolic turnover rate of this radionuclide for the viscera is more rapid than that for the muscle. From the experimental results, it was confirmed that the relative contribution by ingestion of the radioactive diets in the accumulation of {sup 137}Cs in natural environmental fish was comparatively higher in comparison with uptake of the radionuclide in the radioactive freshwater. With regard to the difference between the radioactive diets and the control diets, the accumulation of {sup 137}Cs in whole body of the fish from the radioactive diets was less than that from the control diets to rearing 5 days, but afterward was not different. The difference of the accumulation between these diets will be attributable to that of the metabolic turnover between these diets. (author)

  7. Radioecological studies of {sup 90}Sr in limnological ecosystems. Accumulation and excretion of {sup 85}Sr in goldfish, Carassius auratus auratus, rearing in the radioactive freshwater

    Energy Technology Data Exchange (ETDEWEB)

    Miyake, Sadaaki [Saitama Inst. of Public Health (Japan); Izumo, Yoshiro [Association of Public Health Promotion, Wako, Saitama (Japan)

    2003-01-01

    Accumulation and excretion of {sup 85}Sr in goldfish, Carassius auratus auratus, rearing in the radioactive freshwater were investigated in order to elucidate the accumulation mechanism of {sup 90}Sr in naturally living fishes. The accumulation of {sup 85}Sr, expressed in concentration ratio (CR) between fish and water, in whole body of the fish showed a increasing tendency with the rearing time, and the CR value reached 5.4{+-}0.4 (mean{+-}standard error) at 7th day. On the other hand, the excretion of {sup 85}Sr, expressed in retention rate, in whole body rearing in non-radioactive freshwater following the accumulation above the 7 days demonstrated a rapid decreasing at first few days, and then a gradual decreasing tendency indicating the biological half lives about 4 days and 205 days, respectively. The retention rate resulted in nearly 75% of initial radioactivity, i.e. 25% of excretion, at 25th day. As for the tissues and organs, the CR values of {sup 85}Sr reared in the radioactive freshwater for 7 days were 62.3 (vertebra: bone), 31.1 (scale), 12.5 (gill), 0.6 (viscera) and 0.4 (muscle). On the other hand, higher excretion was found in the viscera and the muscle than that in the bone, the scale and the gill. It is so suggested that the metabolic turnover rate of this radionuclide is different among these tissues and organs particularly characterizing higher accumulation and lower excretion in the vertebra (bone) and scale. (author)

  8. Characterization and expression analysis of B Cell receptor accessory molecule CD79 gene in humphead snapper ( Lutjanus sanguineus)

    Science.gov (United States)

    Huang, Yucong; Yan, Xiuying; Cai, Shuanghu; Cai, Jia; Jian, Jichang; Lu, Yishan; Tang, Jufen; Wu, Zaohe

    2016-04-01

    CD79, a key component of the B cell antigen receptor complex, is composed of CD79α(Igα) and CD79β(Igβ) encoded by mb-1 and B29 respectively, and plays an important role in B cell signaling. In this study, we isolated and characterized mb-1 and B29 from humphead snapper ( Lutjanus sanguineus). Their tissue distribution and expression profiles after stimulations in vitro and in vivo were also investigated. The humphead snapper mb-1 and B29 contain open reading frames of 684 bp and 606 bp, encoding 227 amino acids and 201 amino acids, respectively. Both CD79α and CD79β possess signal peptide, extracellular Ig domain, transmembrane region and immunoreceptor tyrosine kinase activation motif (ITAM). Mb-1 is highly expressed in lymphoid organs (thymus, posterior kidney and spleen) and mucosal-associated lymphoid tissues (gill and intestine), while B29 is mainly detected in posterior kidney, spleen, gill and skin. Furthermore, transcription of mb-1 and B29 in head kidney leucocytes was up-regulated following lipopolysaccharide (LPS), pokeweed mitogen (PWM), and polyinosinic-polycytidylic acid (PolyI:C) stimulation, respectively, and their expression level in anterior kidney and spleen was also increased after challenged with formalin-inactived Vibrio harveyi. These results indicated that humphead snapper CD79 molecule might play an important role in immune response to pathogen infection.

  9. Many branches, one root: First evidence for a monophyly of the morphologically highly diverse goldfish (Carassius auratus)

    Czech Academy of Sciences Publication Activity Database

    Rylková, K.; Kalous, L.; Šlechtová, Vendula; Bohlen, Jörg

    2010-01-01

    Roč. 302, 1-2 (2010), s. 36-41 ISSN 0044-8486 R&D Projects: GA ČR GA206/05/2159 Institutional research plan: CEZ:AV0Z50450515 Keywords : Goldfish * Carassius auratus * Phylogenetics Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.044, year: 2010

  10. Geographic distribution of ebony leaf monkey Trachypithecus auratus (E. Geoffroy Saint-Hilaire, 1812) (Mammalia: Primates: Cercopithecidae)

    NARCIS (Netherlands)

    Nijman, Vincent

    2000-01-01

    As one of the fundamental units of ecology and biogeography, the geographic distribution of the endemic and threatened ebony leaf monkey Trachypithecus auratus (E. Geoffroy Saint-Hilaire, 1812) on the islands of Java, Bali, and Lombok (Indonesia) has been assessed. All localities where the species

  11. Kin discrimination in cannibalistic tadpoles of the Green Poison Frog, Dendrobates auratus (Anura, Dendrobatidae

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    Heather M. Gray

    2009-07-01

    Full Text Available Cannibalizing a related individual can reduce the inclusive fitness of the cannibal. Hence, mechanisms that allow a tadpole to recognize and modify its behavior toward kin may reduce the inclusive fitness costs of cannibalism. Alternatively, ecological factors may cause preferential treatment of kin to be too costly to be favored by selection. We tested these two predictions in the Green Poison Frog, Dendrobates auratus. The effect of kinship on larval cannibalism was examined through a series of kin-discrimination trials. The behavior of large tadpoles was observed when presented with two small, tethered tadpoles, one a clutchmate and one an unrelated tadpole. In these simultaneous presentation tests, tadpoles displayed a significant preference for attacking kin. In a series of timed trials, pairs of unequally sized tadpoles were placed together in containers. The majority (70% of large tadpoles took less than24 hr to consume the small tadpole. Kinship did not affect the survival time of the small tadpole. Our results are consistent with observations that D. auratus is an indiscriminate predator. As conspecifics may be serious competitors, their swift elimination would be an advantage, particularly in the small, nutrient-poor poolsused by this species.

  12. Toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the peripheral nervous system of developing red seabream (Pagrus major)

    Energy Technology Data Exchange (ETDEWEB)

    Iida, Midori [Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577 (Japan); Kim, Eun-Young [Department of Life and Nanopharmaceutical Science and Department of Biology, Kyung Hee University, Seoul 130-701 (Korea, Republic of); Murakami, Yasunori [Graduate School of Science and Engineering, Ehime University, Matsuyama 790-8577 (Japan); Shima, Yasuhiro [National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Imabari 794-2305 (Japan); Iwata, Hisato, E-mail: iwatah@agr.ehime-u.ac.jp [Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577 (Japan)

    2013-03-15

    We investigated 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced effects on the morphology of peripheral nervous system (PNS) in the developing red seabream (Pagrus major) embryos. The embryos at 10 h post-fertilization (hpf) were treated with 0, 0.1, 0.4 or 1.7 μg/L of TCDD in seawater for 80 min. The morphology of PNS was microscopically observed with florescence staining using an anti-acetylated tubulin antibody at 48, 78, 120 and 136 hpf. Axon length of facial nerve (VII) was found to be shortened by TCDD exposure. Axon guidance in the glossopharyngeal nerve (IX) and vagus nerve (X) was altered at 120 and 136 hpf in a TCDD dose-dependent manner. Lowest observable effect level of TCDD (0.1 μg/L) that induced the morphological alteration of PNS was lower than those of other endpoints on morphological deformities so far reported. Given that the growth cone at the tip of growing nerve axons advances under the influence of its surrounding tissues, we hypothesized that TCDD exposure would affect (1) the nerve cell proliferation/differentiation, (2) the structure of muscle as an axon target and (3) the nerve guidance factor in the embryos. By the immunostaining of embryos with an antibody against the neuronal specific RNA-binding protein, HuD, and an antibody against the sarcomeric myosin, no morphological effects were observed on the neural proliferation/differentiation and the structure of facial muscles of TCDD-treated embryos. In contrast, whole mount in situ hybridization of semaphorin 3A (Sema3A), a secretory axon repulsion factor, revealed the altered expression pattern of its transcripts in TCDD-treated embryos. Our findings suggest that TCDD treatment affects the projection of PNS in the developing red seabream embryos through the effects on the axonal growth cone guidance molecule such as Sema3A, but not on the neuronal differentiation/proliferation and axon target. The PNS in developing embryos may be one of the most sensitive biomarkers to the exposure

  13. Enzymatic complex in diets for gold fish fingerlings (Carassius auratusComplexo enzimático na dieta de alevinos de kinguio (Carassius auratus

    Directory of Open Access Journals (Sweden)

    Arcângelo Augusto Signor

    2013-06-01

    Full Text Available Aiming to evaluate the enzymatic complex supplementation in diets for goldfish fingerlings (Carassius auratus, 240 fish weighing initially 1,36 ± 0,02g, randomly distributed in 20 tanks with 150L, in four treatments and five replications, with twelve fish in each experimental unit were used. The fish were fed at 8:00 and 11:00 a.m. and 2:00 and 5:00 p.m. with diets containing different inclusion levels (0; 0,033; 0,066 e 0,099% of enzymatic complex (amilase, protease, celulase, lipase, ?-glucanase and phytase, and formulated with 32,36% of digestible protein and 3.023kcal of digestible energy kg-1. There were no differences observed (P>0,05 in the mean final weight, weight gain, total length, standard length, survival and carcass composition. However, the fish apparent feed conversion was impaired by the supplementation of enzymatic complex with 0,099% in diet. The use of enzymatic complex does not provides benefits in the productive performance for goldfish fingerlings. Objetivando avaliar a suplementação de complexo enzimático em dietas de alevinos de kinguios (Carassius auratus, foram utilizados 240 peixes com peso inicial de 1,36 ± 0,02g, distribuídos aleatoriamente, em 20 tanques de 150L, em quatro tratamentos e cinco repetições, com 12 peixes em cada unidade experimental. Os peixes foram arraçoados as 8h, 11h, 14h e 17h, com dietas contendo diferentes níveis de inclusão (0; 0,033; 0,066 e 0,099% de complexo enzimático (amilase, protease, celulase, lipase, b-glucanase e fitase, e formuladas para conter 32,36% de proteína digestível e 3.023kcal de energia digestível kg-1. Não foram observadas diferenças no peso final, ganho de peso, comprimento total, comprimento padrão, sobrevivência e composição de carcaça. No entanto, a conversão alimentar aparente dos peixes alimentados com rações contendo 0,099% do complexo enzimático foi inferior em relação aos alimentados com as demais dietas. Conclui-se que a utiliza

  14. Biochemical and physicochemical analysis of fish protein isolate recovered from red snapper (Lutjanus sp.) by-product using isoelectric solubilization/precipitation method

    Science.gov (United States)

    Pramono, H.; Pujiastuti, D. Y.; Sahidu, A. M.

    2018-04-01

    The effect of acid- and alkali-process on biochemical and physicochemical characteristics of fish protein isolate from red snapper (Lutjanus sp) by-product was evaluated. Protein recovered by alkali process (16.79%) was higher compared to acid process (13.75%). Reduction of lipid content and total volatile basic nitrogen (TVB-N) exhibited in both treatments indicated both process improved fish protein isolate recovered from red snapper by-product. In addition, the increasing of water holding capacity and oil binding capacity were observed. However, high peroxide value of fish protein isolate was showed in both treatment. This finding indicated that acid and alkali process can be used as a useful method to recover proteins from red snapper by-product. Alkali process gave a protein isolate with better overall quality compared to acid process.

  15. Comparative characterization of protease activity in cultured spotted rose snapper juveniles (Lutjanus guttatus

    Directory of Open Access Journals (Sweden)

    Emyr Peña

    2015-09-01

    Full Text Available Partial characterizations of digestive proteases were studied in three life stages of spotted rose snapper: early (EJ, middle (MJ and late juvenile (LJ with corresponding average weights of 21.3 ± 2.6 g (3 months after hatching, MAH, 190 ± 4.4 g (7 MAH, and 400 ± 11.5 g (12 MAH. At sampling points, the digestive tract was dissected into the stomach (St, pyloric caeca (PC, and the intestine in three sections (proximal (PI, middle (MI and distal intestine (DI. The effect of pH and temperature and specific inhibitors were evaluated for acid and alkaline proteases. Total acid and alkaline protease activity showed a tendency to increase with juvenile life stage of fish while trypsin activity decreased. Differences were found in acid and alkaline protease activities at different pH and temperatures during juvenile stages. Pepstatin A inhibited total activity in the stomach extract in all juvenile stages. Activity in total alkaline protease inhibition was significantly higher in EJ using TLCK, PMSF, SBTI, Phen and Ovo than in MJ and LJ, while no significant differences were found with TPCK inhibition. Therefore increases in protease activities with fish growth through juvenile stages in which a substitution or diversification in the type of alkaline enzymes exist. These results lead a better comprehension of changes in digestive potential of Lutjanidae fish.

  16. The effect of mimosa and syntan mixture on the quality of tanned red snapper leather

    Science.gov (United States)

    Pratama, M.; Sahubawa, L.; Pertiwiningrum, A.; Rahmadian, Y.; Puspita, I. D.

    2018-03-01

    This study aimed to determine the effect of syntan and mimosa combination on the quality of tanned red snapper leather. The study was designed using complete randomized design (CRD) and the advanced test of Least Significant Difference (LSD) with three replications and three treatments of combined syntan and mimosa, namely: p1 (4 % syntan, 5 % mimosa), p2 (8 % syntan, 5 %mimosa), p3 (12 % syntan, 5 % mimosa). Data were analyzed using SPSS 18. The measured parameters were tensile strength (N·cm-2), elongation (%), tear strength (N·cm-1), enervation (mm), wrinkle temperature (°C), fat/oil content (%) and water content (%). The results indicate that the combined syntan and mimosa tanners gave no significant effect on the physical parameters (tensile strength, elongation, tear strength, enervation and wrinkle temperature) and chemical parameters (moisture and fat/oil). All treatments (p1, p2 and p3) met the Indonesian National Standard (SNI) 06-4586-1998 for chrome tanned freshwater snake leather for the parameters of tensile strength, tear strength, enervation, wrinkle temperature and moisture. The parameter of elongation and fat/oil content did not meet with the SNI 06-4586-1998. Among the three combinations of a tanner, syntan 4 % and mimosa 5 % treatment gave the best leather quality.

  17. [Reproductive biology of the golden snapper Lutjanus inermis (Perciformes: Lutjanidae) in the central Mexican Pacific].

    Science.gov (United States)

    Lucano-Ramírez, Gabriela; Ruiz-Ramírez, Salvador; González-Sansón, Gaspar; Ceballos-Vázquez, Bertha Patricia

    2012-03-01

    Reproductive aspects and information on the effect of fishery activities over this species stock are scarce, despite the imperative need of effective protection and resource management programs. To support the possible establishment of closed seasons and catch levels for the area, our study aimed to evaluate the spawning season of the Golden snapper (Lutjanus inermis). For this, fish caught by artisanal fisheries in the Navidad, Tenacatita and Chamela bays (Jalisco, Mexico) were sampled monthly from 1999-2008, and analyzed sex ratios, spawning season and size at first maturity. A total of 311 (53.1%) females and 275 (46.9%) males were caught, and the observed sex ratio was 1:0.88. The total length range was 14.4-37.0cm and total weight range from 48-575g. The length at first maturity was 23.9cm TL for females and 23.6cm TL for males. The gonadosomatic index showed two principal reproductive periods in the year (February-April and September-November), when the largest number of mature gonads were observed. Ovaries were of asynchronic development type and seven oocyte development stages were identified. Testicles showed lobular development and spermatozoa were observed in the center of the cysts and in the collecting ducts. It is important to mention that our results support the current suitable exploitation of this species in the area.

  18. Effect of Protein-Based Edible Coating from Red Snapper (Lutjanus sp.) Surimi on Cooked Shrimp

    Science.gov (United States)

    Rostini, I.; Ibrahim, B.; Trilaksani, W.

    2018-02-01

    Surimi can be used as a raw material for making protein based edible coating to protect cooked shrimp color. The purpose of this study was to determine consumers preference level on cooked shrimp which coated by surimi edible coating from red snapper and to know the microscopic visualization of edible coating layer on cooked shrimp. The treatments for surimi edible coating were without and added by sappan wood (Caesalpinia sappan Linn) extract. Application of surimi edible coating on cooked shrimp was comprised methods (1) boiled then coated and (2) coated then boiled. Edible coating made from surimi with various concentrations which were 2, 6, 10 and 14% of distillated water. The analysis were done using hedonic test and microscopic observation with microscope photographs. Effect of surimi edible coating on cooked shrimp based on the hedonic and colour test results showed that the 14% surimi concentration, added by sappan wood (Caesalpinia sappan Linn) extract on edible coating was the most preferable by panellist and giving the highest shrimp colour. The edible coating surimi application on cooked shrimp which gave the best result was processed by boiling followed by coating.

  19. MALATHION INDUCED HISTOLOGICAL MODIFICATIONS IN GILLS AND KIDNEY OF CARASSIUS AURATUS GIBELIO

    Directory of Open Access Journals (Sweden)

    ANDREA CRISTINA STAICU

    2008-05-01

    Full Text Available Malathion is an organophosphorous insecticide, used in agriculture and a possible source of environmental poisoning. During malathion metabolization, mitochondria generates reactive oxygen species, responsible for significant structural changes. In this study, gills and kidney histological changes in Carassius auratus gibelio exposed to 0.05 mg/l malathion were investigated. In kidney, the effects were dramatic. The 24 -72 hours exposure to malathion induced cytoplasm vacuolization and changes in cell and nuclear volumes. In addition, necrotic renal tubules appeared, nuclear malformations of epithelial cells, anisokary, nuclei pycnosis and nuclei hypertrophy, were noticed. Epithelial ruptures, secondary lamellae fusion and hyperplasia of branchial epithelium, vascular congestion were the main changes noticed soon after pollutant exposure. We suggest that structural changes in gill and kidney could be used as good response to aquatic pollution with organophosphorous insecticides.

  20. Hamsters' (Mesocricetus auratus) memory in a radial maze analog: the role of spatial versus olfactory cues.

    Science.gov (United States)

    Tonneau, François; Cabrera, Felipe; Corujo, Alejandro

    2012-02-01

    The golden hamster's (Mesocricetus auratus) performance on radial maze tasks has not been studied a lot. Here we report the results of a spatial memory task that involved eight food stations equidistant from the center of a circular platform. Each of six male hamsters depleted the food stations along successive choices. After each choice and a 5-s retention delay, the hamster was brought back to the center of the platform for the next choice opportunity. When only one baited station was left, the platform was rotated to evaluate whether olfactory traces guided hamsters' choices. Results showed that despite the retention delay hamsters performed above chance in searching for food. The choice distributions observed during the rotation probes were consistent with spatial memory and could be explained without assuming guidance by olfactory cues. The radial maze analog we devised could be useful in furthering the study of spatial memory in hamsters.

  1. Cause and Possible Treatments of Foot Lesions in Captive Syrian Hamsters (Mesocricetus auratus

    Directory of Open Access Journals (Sweden)

    Mélisa Veillette

    2010-01-01

    Full Text Available Syrian hamsters (Mesocricetus auratus run extensively in exercise wheels. This running may cause paw lesions. Three treatments of these wounds (topical application of vitamin E, wheel blocking, and a combination of both were compared using both sexes. A pretreatment period with or without wheels lasted 15 days and the ensuing treatment period lasted 45 days. At the end of the pre-treatment period, none of the animals without wheels had paw wounds, whereas at least 75% of the females and 100% of the males with wheels did. Females had fewer and smaller wounds than males at this point. At the end of the treatment period, no effect of vitamin E could be discerned, but significant wound healing occurred after wheel blocking in both males and females. Wheel blocking is an easy way to prevent or treat paw wounds, but it presents problems in terms of animal welfare, as wheels are an important cage enrichment for hamsters.

  2. Memory for individual scent in hamsters (Mesocricetus auratus) as assessed by habituation methods.

    Science.gov (United States)

    Johnston, R E

    1993-06-01

    The memory of hamsters (Mesocricetus auratus) for the flank scent of other male hamsters was investigated in a series of habituation experiments. In 2 types of habituation tasks (Experiments 1 and 2), male hamsters habituated to the flank scent of 1 male and then increased their level of investigation to that of a novel male; similar results were obtained when the intervals between trials ranged from 1 s to 2 days. When the test trial was 10 or 21 days after habituation (Experiment 3), males discriminated between familiar and novel flank scents at 10 days but not at 21 days. The results demonstrate recognition of familiar and unfamiliar individual odors and excellent memory for these differences. Habituation techniques yield extremely robust results and may be useful for investigations of other aspects of individual signatures.

  3. Complete genome sequence and architecture of crucian carp Carassius auratus herpesvirus (CaHV).

    Science.gov (United States)

    Zeng, Xiao-Tao; Chen, Zhong-Yuan; Deng, Yuan-Sheng; Gui, Jian-Fang; Zhang, Qi-Ya

    2016-12-01

    Crucian carp Carassius auratus herpesvirus (CaHV) was isolated from diseased crucian carp with acute gill hemorrhages and high mortality. The CaHV genome was sequenced and analyzed. The data showed that it consists of 275,348 bp and contains 150 predicted ORFs. The architecture of the CaHV genome differs from those of four cyprinid herpesviruses (CyHV1, CyHV2, SY-C1, CyHV3), with insertions, deletions and the absence of a terminal direct repeat. Phylogenetic analysis of the DNA polymerase sequences of 17 strains of Herpesvirales members, and the concatenated 12 core ORFs from 10 strains of alloherpesviruses showed that CaHV clustered together with members of the genus Cyprinivirus, family Alloherpesviridae.

  4. BIOCHEMICAL AND HISTOLOGICAL EFFECTS OF DELTAMETHRIN EXPOSURE ON THE GILLS OF CARASSIUS AURATUS GIBELIO (Pisces Cyprinidae

    Directory of Open Access Journals (Sweden)

    DIANA COSTIN

    2007-05-01

    Full Text Available This study investigated the alterations in the activities of several antioxidant enzymes inthe gills of the freshwater fish Carassius auratus gibelio exposed to deltamethrin. To getthis goal, groups of 10 individuals were exposed for one, two, three, seven and fourteendays to sublethal concentration of deltamethrin (2 µg/L. Another group was used ascontrol. The activities of catalase, gluthatione peroxidase and gluthatione reductasewere significantly decreased, while the glutathione-S-transferase was up-regulated. Allfish, exposed to 2 µg/L deltamethrin revealed gills morphological alterations after 48h ofexposure which were accentuated after 14 days. In the gills hyperemia, fusion ofsecondary lamellae, epithelial layer rupture and chloride cells proliferation wereobserved. These results suggest that an immediate adaptive response to the oxidativestress appeared, demonstrating alterations in the antoxidant defense mechanism in thegills of deltamethrin intoxicated fish.

  5. LDH ACTIVITY IN COPPER INTOXICATION OF CARASSIUS AURATUS GIBELIO GILLS AND INTESTINE

    Directory of Open Access Journals (Sweden)

    DANIELA TEODORESCU

    2008-05-01

    Full Text Available The pathological effects of two sublethal concentrations (100 μg/l and 250 μg/l of copper (CuSO4x5H2O on goldfish Carassius auratus gibelio were studied for 7,14 and 21 days. The specific activity of LDH in gills and intestine, two target organs that uptake the metal from the water were assayed. In gills at 100 μg Cu2+/l the specific activity of LDH was gradually decreasing, while in the intestine, after 7 days of exposure, the enzymatic activity was distinct significantly increased. LDH activity demonstrated a hypoxic condition and a stimulation of glycolysis. In the both organs ,the 250 μg Cu2+/l concentration generated a decrease of LDH specific activity after 7 days followed by an increase of this after 14 and 21 days of exposure. Histologically, the modifications are, generally, directly correlated with the toxicant dose and exposure time.

  6. Negative effect of 17-beta-estradiol on growth parameters of goldfish (Carassius auratus

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    Reza Tarkhani

    2015-03-01

    Full Text Available Objective: To evaluate the effects of 17-beta-estradiol on growth factors of goldfish (Carassius auratus. Methods: To perform the test, 17-beta-estradiol was given 3 months period to fish at different doses as followed: control group, Group 1: 10 mg/kg food, Group 2: 25 mg/kg food and Group 3: 50 mg/kg food. For this purpose, a solution of hormone in pure ethanol used to spray on food. Feeding was done 3 times daily as an appetite. Comparing the mean values measured for length and weight using ANOVA. Results: Indicated with increase length and weight, the effects of the hormone get more distinct, so that with increase concentration of hormone, reduce weight and length. Conclusions: Estradiol along with testosterone and progesterone regulates final stages of oocyte maturation and ovulation. Various studies have proven the different concentrations of this hormone has different effects on the growth of different fishes.

  7. Variation in myelin lipid composition induced by change in environmental temperature of goldfish (Carassius auratus L. )

    Energy Technology Data Exchange (ETDEWEB)

    Selivonchick, D.P.; Roots, B.I.

    1976-04-01

    Goldfish (Carassius auratus L.) were acclimated to 5, 15, and 30/sup 0/C, and the lipid and protein composition of brain and spinal cord myelin was determined. Goldfish myelin contains less galactolipid, but more protein and phospholipid than mammalian and bird myelin. Phosphatidyl choline was the predominant phospholipid in both brain and spinal cord myelin. Fish myelin also showed a greater plasmalogen content with an average ethanolamine plasmalogen/total phosphatidyl ethanolamine ratio of 0.84. Total brain and myelin lipids, with the exception of plasmalogens, showed a resistance to change with thermal acclimation. Differences between brain and spinal cord myelin protein and phospholipids were not observed. It is suggested that temperature acclimation in poikilotherms may be used as a tool in the study of membrane adaptability.

  8. THE ACTION OF CORAGEN INSECTICIDE ON CERTAIN PHYSIOLOGICAL BIOMARKERS ON CARASSIUS AURATUS GIBELIO BLOCH L. 1758

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    Claudiu Alexandru Baciu

    2015-12-01

    Full Text Available In our researches we have determined the variation of certain physiological indexes, such as the oxygen consume, the breathing rhythm, the glycaemia and the number of red blood cells under the action of Coragen insecticide on Carassius auratus gibelio Bloch. Under the action of Coragen, we have registered significant changes in the oxygen consume, the breathing rhythm, the number of red blood cells and glycemia at the Carassius auratus gibelio Bloch items, considered as answers to the stress provoked by emissions. The highest variations of the physiological indexes, from the perspective of the percentage, were noticed at the glycemia, which at the mark was 28 mg/dl, and in the treated sample, with 0.1 ml/l Coragen is 42 mg/dl, representing a 50% growth and at the breathing rhythm in 24 hours, where values significantly decreased with 41.18% at the concentration of 0.07 ml/l and with 39.33% at the concentrations of 0.05 and 0.1 ml/l Coragen. The slightest variations of the physiological indexes, from the perspective of percentage, were noticed at the oxygen consumption, which, at the mark is of 55.302 ml oxygen/kg/hour, and for the treated sample, with 0.1 ml/l Coragen is 34.81 ml oxygen/kg/hour, representing a decrease of 37.06% in 24 hours and the number of red blood cells, where the values have significantly decrease with 9.58%, 13.48%, respectively 18.44% for the concentrations of 0.05, 0.07 and 0.1 ml/l Coragen.

  9. Cucullanus pargi sp. n. (Nematoda: Cucullanidae) from the grey snapper Lutjanus griseus off the southern coast of Quintana Roo, Mexico.

    Science.gov (United States)

    González-Solís, David; Tuz-Paredes, Vielka M; Quintal-Loria, Miguel A

    2007-09-01

    A new nematode species, Cucullanus pargi sp. n., is described from the intestine and pyloric caeca of the grey snapper, Lutjanus griseus (Linnaeus), off the southern Quintana Roo coast, Mexico. This species shows similar morphological features as cucullanids occurring in marine and brackish-water fishes; however, it differs from all other species in the length of spicules, arrangement and number of caudal papillae, position of the excretory pore and deirids. Cucullanus pargi is the third species of this genus described from fishes in Mexico and the second one from Mexican marine fishes.

  10. Laboratory evaluation of different formulations of Stress Coat? for slime production in goldfish (Carassius auratus) and koi (Cyprinus carpio)

    OpenAIRE

    Shivappa, Raghunath B.; Christian, Larry S.; Law, Jerry M.; Lewbart, Gregory A.

    2017-01-01

    A study was carried out to assess the effect of Stress Coat® on slime production in goldfish (Carassius auratus) and koi (Cyprinus carpio). The study also investigated histological changes that might be associated with slime producing cells, and wound healing in koi. Several formulations of Stress Coat® were investigated and the results showed that polyvinylpyrrolidone (PVP), also known as povidone, an ingredient of Stress Coat®, when used alone, showed significantly higher slime production i...

  11. Changes of ploidy and sexuality status of "Carassius auratus" populations in the drainage area of the River Dyje (Czech Republic)

    Czech Academy of Sciences Publication Activity Database

    Lusková, Věra; Halačka, Karel; Vetešník, Lukáš; Lusk, Stanislav

    2004-01-01

    Roč. 4, č. 2 (2004), s. 165-171 ISSN 1642-3593. [International Symposium on the Ecology of Fluvial Fishes /9./. Lodz, 23.06.2003-26.06.2003] R&D Projects: GA ČR GA206/00/0668; GA AV ČR IBS5045111 Institutional research plan: CEZ:AV0Z6093917 Keywords : Carassius auratus * goldfish * ploidy status Subject RIV: EH - Ecology, Behaviour

  12. TINGKAH LAKU MAKAN LUTUNG JAWA Trachypithecus auratus DI KAWASAN PANCURAN 7 BATURADEN GUNUNG SLAMET JAWA TENGAH

    OpenAIRE

    Dwi Eliana; Erie Kolya Nasution; Indarmawan Indarmawan

    2017-01-01

    Javan Langur (Trachypithecus auratus) has an important ecological role in conserving the forest as their habitat by spreading seeds of fruits of their food. They are also responsible for the pattern of the plant diversity and forest regeneration as their habitat. Unfortunately, Javan Langur has been listed on Appendix II of CITES and categorized as vulnerable by the IUCN. Pancuran 7 Baturaden on the Mount Slamet slope is one of the few Javan Langur habitats in Java. Information on the Javan L...

  13. Javan Leaf Monkey (Trachypithecus Auratus) Movement in a Fragmented Habitat, at Bromo Tengger Semeru National Park, East Java, Indonesia

    OpenAIRE

    Subarkah, M. Hari; Wawandono, Novianto Bambang; Pudyatmoko, Satyawan; Subeno, Subeno; Nurvianto, Sandy; Budiman, Arif

    2011-01-01

    Pergerakan Lutung budeng (Trachypithecus auratus) didaerah habitat terfragmentasi Taman Nasional Bromo Tengger Semeru, Jawa Timur, Indonesia. Pergerakan lutung budeng di daerah habitat terfragmentasi diamati dengan metode transek. Hasil kajian menunjukkan bahwaada empat kelompok masing masing beranggotakan 12 (grup A), 16 (grup B), 15 (grup C) dan 12 lutung (grup D). Penelitian yang dilakukan disekitar hunian penduduk, jalan, hutan terdegradasi dan jalan-jalan setapak mengindikasikan bahwa lu...

  14. Geographic distribution of ebony leaf monkey Trachypithecus auratus (E. Geoffroy Saint-Hilaire, 1812) (Mammalia: Primates: Cercopithecidae)

    OpenAIRE

    Nijman, Vincent

    2000-01-01

    As one of the fundamental units of ecology and biogeography, the geographic distribution of the endemic and threatened ebony leaf monkey Trachypithecus auratus (E. Geoffroy Saint-Hilaire, 1812) on the islands of Java, Bali, and Lombok (Indonesia) has been assessed. All localities where the species has been collected are listed, and forty-two areas (each in itself consisting of numerous smaller sites) where the species has been recorded are discussed. The species occurs in a large variety of f...

  15. Common carp (Cyprinus carpio) and goldfish (Carassius auratus) were not susceptible to challenge with ranavirus under certain challenge conditions

    DEFF Research Database (Denmark)

    Jensen, Britt Bang; Reschova, S.; Cinkova, K.

    2011-01-01

    Goldfish, Carassius auratus, and common carp, Cyprinus carpio, were challenged with a panel of 8 different piscine and amphibian ranavirus isolates. Goldfish were exposed at a water temperature of 16 degrees C and 23 degrees C, and carp at 15 degrees C and 25 degrees C. No significant mortality...... was registered, and virus could not be re-isolated from goldfish or carp bath-challenged with any of the isolates in the panel....

  16. Severe glomerular disease in juvenile grey snapper Lutjanus griseus L. in the Gulf of Mexico caused by the myxozoan Sphaerospora motemarini n. sp

    Czech Academy of Sciences Publication Activity Database

    Holzer, Astrid S.; Pecková, Hana; Patra, Sneha; Brennan, N.P.; Yanes-Roca, C.; Main, K.L.

    2013-01-01

    Roč. 2, - (2013), s. 124-130 ISSN 2213-2244 Grant - others:GA AV ČR(CZ) M200961250 Institutional support: RVO:60077344 Keywords : Mangrove snapper * Fisheries * Juvenile pathology * Glomerulonephritis * Mortality * Myxozoa Subject RIV: EA - Cell Biology

  17. Influence of the temperature on the early larval development of the Pacific red snapper, Lutjanus peru (Nichols & Murphy, 1922

    Directory of Open Access Journals (Sweden)

    José Antonio Estrada-Godínez

    2015-03-01

    Full Text Available The Pacific red snapper, Lutjanus peru, is a commercially important species throughout its distribution range, making it a good alternative for aquaculture; however, there is few information regarding environmental conditions and their influence on early development of this species. Temperature is one of the main factors affecting embryo and larval development in marine fishes. In this paper, the effects of different temperatures upon hatching rate, growth, consumption of yolk sac and oil droplet and the formation of the digestive system and eye pigmentation were evaluated in larvae of this species under experimental conditions. Eggs incubated between 20 and 32°C showed hatching rates higher than 90%. However, larvae maintained at 26°C showed significantly larger notochord length and were the first to complete the pigmentation of the eyes and the formation of the digestive system when still possessing enough reserves in the yolk sac. Therefore, according to the results obtained, it is recommended that the incubation of eggs and larval rearing in Pacific red snapper takes place between 25 and 26°C.

  18. Morphology and morphometric relationships of the sagitta of Diapterus auratus (Perciformes: Gerreidae from Veracruz, Mexico

    Directory of Open Access Journals (Sweden)

    Verónica Rivera Félix

    2013-03-01

    Full Text Available Sagittae otoliths are the most studied because of their morphological variability and size; the sagittae may also have valuable taxonomic use and for D. auratus has not yet been described. In the present study we present a morphological description of the sagitta otolith and an analysis of its correlation with various morphometric parameters. The biological material was captured with a beach seine in the coasts of Alvarado and Port of Veracruz, from November 2009 to June 2010. Sagittae were extracted, cleaned, photographed (SEM and optical, and measured. The analysis included the correlations between sagitta’s length and width, versus the fish standard length, height and weight, for a total of 449 fishes. The comparison between left and right sagitta showed no significant difference in both otolith length and width (t-tests, pLos otolitos sagitta son los más estudiados debido a su variabilidad morfológica y a su tamaño, sin embargo, los otolitos de D. auratus no han sido estudiados hasta el momento. En el presente estudio, se muestra una descripción completa de la morfología y un análisis morfométrico de diversas medidas. El análisis incluye las relaciones entre el ancho y largo de la sagitta contra la longitud cefálica, longitud estándar, altura y peso de 449 peces. El material biológico fue capturado con un chinchorro playero en las costas de Alvarado y el Puerto de Veracruz de noviembre 2009 a junio 2010. Las sagittae fueron extraídas, limpiadas, fotografiadas (SEM y ópticas y medidas. La comparación entre el otolito derecho e izquierdo no mostró diferencias significativas entre el ancho y largo (prueba de t, p<0.05 para cada sexo. El otolito derecho fue utilizado para las relaciones de las distintas medidas del otolito del pez y del otolito de hembras y machos; la prueba de Fisher (d.f. 161 and 143, p<0.001 no mostró diferencias significativas de las pendientes e intersecciones entre ellos. La relación longitud del otolito

  19. High connectivity in the deepwater snapper Pristipomoides filamentosus (Lutjanidae) across the Indo-Pacific with isolation of the Hawaiian archipelago.

    Science.gov (United States)

    Gaither, Michelle R; Jones, Shelley A; Kelley, Christopher; Newman, Stephen J; Sorenson, Laurie; Bowen, Brian W

    2011-01-01

    In the tropical Indo-Pacific, most phylogeographic studies have focused on the shallow-water taxa that inhabit reefs to approximately 30 m depth. Little is known about the large predatory fishes, primarily snappers (subfamily Etelinae) and groupers (subfamily Epinephelinae) that occur at 100-400 m. These long-lived, slow-growing species support fisheries across the Indo-Pacific, yet no comprehensive genetic surveys within this group have been conducted. Here we contribute the first range-wide survey of a deepwater Indo-Pacific snapper, Pristipomoides filamentosus, with special focus on Hawai'i. We applied mtDNA cytochrome b and 11 microsatellite loci to 26 samples (N=1,222) collected across 17,000 km from Hawai'i to the western Indian Ocean. Results indicate that P. filamentosus is a highly dispersive species with low but significant population structure (mtDNA Φ(ST)=0.029, microsatellite F(ST)=0.029) due entirely to the isolation of Hawai'i. No population structure was detected across 14,000 km of the Indo-Pacific from Tonga in the Central Pacific to the Seychelles in the western Indian Ocean, a pattern rarely observed in reef species. Despite a long pelagic phase (60-180 days), interisland dispersal as adults, and extensive gene flow across the Indo-Pacific, P. filamentosus is unable to maintain population connectivity with Hawai'i. Coalescent analyses indicate that P. filamentosus may have colonized Hawai'i 26 K-52 K y ago against prevailing currents, with dispersal away from Hawai'i dominating migration estimates. P. filamentosus harbors low genetic diversity in Hawai'i, a common pattern in marine fishes, and our data indicate a single archipelago-wide stock. However, like the Hawaiian Grouper, Hyporthodus quernus, this snapper had several significant pairwise comparisons (F(ST)) clustered around the middle of the archipelago (St. Rogatien, Brooks Banks, Gardner) indicating that this region may be isolated or (more likely) receives input from Johnston Atoll to

  20. Oceanic, Latitudinal, and Sex-Specific Variation in Demography of a Tropical Deepwater Snapper across the Indo-Pacific Region

    Directory of Open Access Journals (Sweden)

    Ashley J. Williams

    2017-12-01

    Full Text Available Deepwater tropical fisheries provide an important source of income and protein to Pacific and Indian Ocean coastal communities who are highly dependent on fish for food security. The development of quantitative assessments and management strategies for these deepwater fisheries has been hindered by insufficient biological and fisheries data. We examine the age-specific demography of the pygmy ruby snapper Etelis carbunculus, an important target species in tropical deepwater fisheries, across 90° of longitude and 20° of latitude in the Pacific and Indian Oceans. Our results show that growth of E. carbunculus varies significantly between oceans and sexes and across latitudes in both oceans. Estimates of natural and fishing mortality were similar between oceans, but higher for females than males in both oceans. Evidence of greater fishing pressure on females than males is likely due to the larger size-at-age of females compared to males, assuming that selectivity of the fishing gear is related directly to fish size. Sex ratios were significantly female biased in both oceans despite this species being gonochoristic, and maturity schedules were similar between sexes in the Pacific Ocean. This species exhibits a protracted spawning season from mid-spring to autumn (i.e., October to May in the Pacific Ocean. These results represent the first estimates of age-specific demographic parameters for E. carbunculus, and provide the foundation for the development of the first species-specific assessment models and harvest strategies for the species. Future stock assessment models for E. carbunculus should consider sex-specific demographic parameters and spatial variation in demography. Our results reveal substantial differences in biology between E. carbunculus and the giant ruby snapper E. sp., a cryptic congeneric species, and thus contribute to greater clarity in managing fisheries that are dependent on these two species. Furthermore, the improved

  1. High connectivity in the deepwater snapper Pristipomoides filamentosus (Lutjanidae across the Indo-Pacific with isolation of the Hawaiian archipelago.

    Directory of Open Access Journals (Sweden)

    Michelle R Gaither

    Full Text Available In the tropical Indo-Pacific, most phylogeographic studies have focused on the shallow-water taxa that inhabit reefs to approximately 30 m depth. Little is known about the large predatory fishes, primarily snappers (subfamily Etelinae and groupers (subfamily Epinephelinae that occur at 100-400 m. These long-lived, slow-growing species support fisheries across the Indo-Pacific, yet no comprehensive genetic surveys within this group have been conducted. Here we contribute the first range-wide survey of a deepwater Indo-Pacific snapper, Pristipomoides filamentosus, with special focus on Hawai'i. We applied mtDNA cytochrome b and 11 microsatellite loci to 26 samples (N=1,222 collected across 17,000 km from Hawai'i to the western Indian Ocean. Results indicate that P. filamentosus is a highly dispersive species with low but significant population structure (mtDNA Φ(ST=0.029, microsatellite F(ST=0.029 due entirely to the isolation of Hawai'i. No population structure was detected across 14,000 km of the Indo-Pacific from Tonga in the Central Pacific to the Seychelles in the western Indian Ocean, a pattern rarely observed in reef species. Despite a long pelagic phase (60-180 days, interisland dispersal as adults, and extensive gene flow across the Indo-Pacific, P. filamentosus is unable to maintain population connectivity with Hawai'i. Coalescent analyses indicate that P. filamentosus may have colonized Hawai'i 26 K-52 K y ago against prevailing currents, with dispersal away from Hawai'i dominating migration estimates. P. filamentosus harbors low genetic diversity in Hawai'i, a common pattern in marine fishes, and our data indicate a single archipelago-wide stock. However, like the Hawaiian Grouper, Hyporthodus quernus, this snapper had several significant pairwise comparisons (F(ST clustered around the middle of the archipelago (St. Rogatien, Brooks Banks, Gardner indicating that this region may be isolated or (more likely receives input from Johnston

  2. Significant improvement of intestinal microbiota of gibel carp (Carassius auratus gibelio) after traditional Chinese medicine feeding.

    Science.gov (United States)

    Wu, Z B; Gatesoupe, F-J; Li, T T; Wang, X H; Zhang, Q Q; Feng, D Y; Feng, Y Q; Chen, H; Li, A H

    2018-03-01

    Increasing attention has been attracted to intestinal microbiota, due to interactions with nutrition, metabolism and immune defence of the host. Traditional Chinese medicine (TCM) feed additives have been applied in aquaculture to improve fish health, but the interaction with fish gut microbiota is still poorly understood. This study aimed to explore the effect of adding TCM in feed on the intestinal microbiota of gibel carp (Carassius auratus gibelio). Bacterial communities of 16 fish intestinal contents and one water sample were characterized by high-throughput sequencing and analysis of the V4-V5 region of the 16S rRNA gene. The results showed that the composition and structure of the bacterial community were significantly altered by the TCM feeding. Some phyla increased markedly (Proteobacteria, Actinobacteria, Acidobacteria, etc.), while Fusobacteria were significantly reduced. Concurrently, the richness and diversity of the taxonomic units increased, and the microbiota composition of TCM-treated fish was more homogeneous among individuals. At the genus level, the addition of TCM tended to reduce the incidence of potential pathogens (Aeromonas, Acinetobacter and Shewanella), while stimulating the emergence of some potential probiotics (Lactobacillus, Lactococcus, Bacillus and Pseudomonas). These data suggested that the feed additive could regulate the fish intestinal microbiota by reinforcing the microbial balance. This study may provide useful information for further application of TCM for diseases prevention and stress management in aquaculture. © 2017 The Society for Applied Microbiology.

  3. The Aggregation of Boron on the Tissues of Gold Fish (Carassius auratus Linnaeus, 1758

    Directory of Open Access Journals (Sweden)

    Tuncer Okan Genç

    2015-03-01

    Full Text Available In this study, it was aimed to determine the water-borne and food-borne boron accumulation in the liver and muscle tissues of Gold Fish (Carassius auratus Linnaeus, 1758. For each treatment, 12 individuals were. The water-borne boron treatments were applied as boron acid concentration of 1 mg/L, 10 mg/L and 20 mg/L in the aquarium water, while the food-borne boron treatments were prepared food contained the defined levels of boron (1 mg/kg, 5 mg/kg and 10 mg/kg as boric acid. The boron levels in the tissues were determined by an ICP-MS procedure. The maximum boron concentration was found in the 20mg/L water borne boron treatment in the liver tissue (1.78±0.02 mg/kg. In the water-borne boron treatments, the maximum Transfer Factor (TF was found in the 20mg/L boron concentration, and TF values were increased when the boron concentrations were decreasing. In the 1 mg/kg food-borne boron treatment, TF was found as 0, and increasing concentration of boron in the food caused an increase in TF reached about 0.06. This study suggested that the target organ for boron accumulation is the liver rather than the muscles and the accumulation of food-borne boron is lower when comparing water-borne boron.

  4. Pharmacokinetics of enrofloxacin after oral, intramuscular and bath administration in crucian carp (Carassius auratus gibelio).

    Science.gov (United States)

    Shan, Q; Fan, J; Wang, J; Zhu, X; Yin, Y; Zheng, G

    2018-02-01

    The pharmacokinetics of enrofloxacin (ENR) was studied in crucian carp (Carassius auratus gibelio) after single administration by intramuscular (IM) injection and oral gavage (PO) at a dose of 10 mg/kg body weight and by 5 mg/L bath for 5 hr at 25°C. The plasma concentrations of ENR and ciprofloxacin (CIP) were determined by HPLC. Pharmacokinetic parameters were calculated based on mean ENR or CIP concentrations using WinNonlin 6.1 software. After IM, PO and bath administration, the maximum plasma concentration (C max ) of 2.29, 3.24 and 0.36 μg/ml was obtained at 4.08, 0.68 and 0 hr, respectively; the elimination half-life (T 1/2β ) was 80.95, 62.17 and 61.15 hr, respectively; the area under the concentration-time curve (AUC) values were 223.46, 162.72 and 14.91 μg hr/ml, respectively. CIP, an active metabolite of enrofloxacin, was detected and measured after all methods of drug administration except bath. It is possible and practical to obtain therapeutic blood concentrations of enrofloxacin in the crucian carp using IM, PO and bath immersion administration. © 2017 John Wiley & Sons Ltd.

  5. Effect of cooking temperatures on protein hydrolysates and sensory quality in crucian carp (Carassius auratus) soup.

    Science.gov (United States)

    Zhang, Jinjie; Yao, Yanjia; Ye, Xingqian; Fang, Zhongxiang; Chen, Jianchu; Wu, Dan; Liu, Donghong; Hu, Yaqin

    2013-06-01

    Cooking methods have a significant impact on flavour compounds in fish soup. The effects of cooking temperatures (55, 65, 75, 85, 95, and 100 °C) on sensory properties and protein hydrolysates were studied in crucian carp (Carassius auratus) soup. The results showed that the soup prepared at 85 °C had the best sensory quality in color, flavour, amour, and soup pattern. Cooking temperature had significant influence on the hydrolysis of proteins in the soup showed by SDS-PAGE result. The contents of water soluble nitrogen (WSN) and non-protein nitrogen (NPN) increased with the cooking temperature, but the highest contents of total peptides and total free amino acids (FAA) were obtained at the cooking temperature of 85 °C. The highest contents of umami-taste active amino acid and branched-chain amino acids were also observed in the 85 °C sample. In conclusion, a cooking temperature of 85 °C was preferred for more excellent flavor and higher nutritional value of crucian carp soup.

  6. Comparative aspects of calcium dynamics in calcified tissues in the goldfish Carassius auratus

    International Nuclear Information System (INIS)

    Ichii, Taro; Mugiya, Yasuo

    1983-01-01

    The comparative nature of calcium physiology in bone, scales and otoliths was studied in young goldfish, Carassius auratus. Net calcium uptake by the fish was estimated to be 143 μg/g body weight/day. Of that, 79 % was distributed in bone, 13 % in scales, 3.5 % in otoliths and 4.5 % in soft tissues. Scales showed the highest incorporation of 45 Ca per mg-tissue weight after 1 or 2 days in 45 Ca-containing water; bone came second and otoliths last. However, 35 days after transfer to non-radioactive water, the order of descending radioactivity had changed to otoliths, bone and scales, reflecting different rates of calcium turnover. In bone, prelabeled 45 Ca activity increased for the first 2 days after transfer and then decreased gradually (biological half-life, Tsub(0.5) = 94 days). In otoliths, prelabeled radioactivity consistently increased for 35 days. Scales showed two phases of calcium turnover. They lost about 33 % of their prelabeled radioactivity during the first 7 days (Tsub(0.5) = 10.5 days) in non-radioactive water, but thereafter the rate of decrease slowed down greatly (Tsub(0.5) = 210 days). These two phases of calcium turnover were found in the osseous layer (including calcium crystals in the fibrillary plate) of scales, indicating the presence of physiologically labile as well as stable forms of calcium in the layer. (author)

  7. Tributyltin disrupts feeding and energy metabolism in the goldfish (Carassius auratus).

    Science.gov (United States)

    Zhang, Jiliang; Sun, Ping; Yang, Fan; Kong, Tao; Zhang, Ruichen

    2016-06-01

    Tributyltin (TBT) can induce obesogen response. However, little is known about the adverse effects of TBT on food intake and energy metabolism. The present study was designed to investigate the effects of TBT, at environmental concentrations of 2.44 and 24.4 ng/L (1 and 10 ng/L as Sn), on feeding and energy metabolism in goldfish (Carassius auratus). After exposure for 54 d, TBT increased the weight gain and food intake in fish. The patterns of brain neuropeptide genes expression were in line with potential orexigenic effects, with increased expression of neuropeptide Y and apelin, and decreased expression of pro-opiomelanocortin, ghrelin, cocaine and amphetamine-regulated transcript, and corticotropin-releasing factor. Interestingly, the energy metabolism indicators (oxygen consumption, ammonia exertion and swimming activity) and the serum thyroid hormones were all significantly increased at the 2.44 ng/L TBT group in fish. However, no changes of energy metabolism indicators or a decrease of thyroid hormones was found at the 24.4 ng/L TBT group, which indicated a complex disrupting effect on metabolism of TBT. In short, TBT can alter feeding and energy metabolism in fish, which might promote the obesogenic responses. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. Bioaccumulation, Subacute Toxicity, and Tissue Distribution of Engineered Titanium Dioxide Nanoparticles in Goldfish (Carassius auratus

    Directory of Open Access Journals (Sweden)

    Mehmet Ates

    2013-01-01

    Full Text Available The increased use of nanosized materials is likely to result in the release of these particles into the environment. It is, however, unclear if these materials are harmful to aquatic animals. In this study, the sublethal effects of exposure of low and high concentrations of titanium dioxide nanoparticles (TiO2 NPs on goldfish (Carassius auratus were investigated. Accumulation of TiO2 NPs increased from 42.71 to 110.68 ppb in the intestine and from 4.10 to 9.86 ppb in the gills of the goldfish with increasing exposure dose from 10 to 100 mg/L TiO2 NPs. No significant accumulation in the muscle and brain of the fish was detected. Malondialdehyde as a biomarker of lipid oxidation was detected in the liver of the goldfish. Moreover, TiO2 NPs exposure inhibited growth of the goldfish. Although there was an increase (8.1% in the body weights of the goldfish for the control group, in the low and high exposure groups 1.8% increase and 19.7% decrease were measured, respectively. The results of this study contribute to the current understanding of the potential ecotoxicological effects of nanoparticles and highlight the importance of characterization of NPs in understanding their behavior, uptake, and effects in aquatic systems and in fish.

  9. Effects of dietary antioxidant of tomato extract and lycopene on Carassius auratus and Xiphophorus maculatus

    Directory of Open Access Journals (Sweden)

    Cynthia Montoya M.

    2014-06-01

    Full Text Available Objective. Evaluate the effect on tegument pigmentation, survival, growth and antioxidant capacity in diets supplemented with tomato extract and lycopene as additives in experimental feed for Carassius auratus and Xiphophorus maculatus. Materials and methods. The additives were added in different concentrations to a basic diet. We performed beginning and an ending biometrics for 100% of the population in each bioassay. The growth and survival of organisms were evaluated. The antioxidant capacity was analyzed by ABTS assay, both in the tomato extract sample as well as in foods used in different bioassays. The concentration of lycopene was determined in food and liver and muscle samples of fish fed with it. Acquired pigmentation of fish was assessed through photographs analyzed with Adobe Photoshop®. The results were evaluated by analysis of variance, and when differences were found (p0.05 on pigmentation and growth of the organisms under the established experimental conditions was obtained. Significant differences in antioxidant capacity (p<0.05 were obtained in foods with added lycopene. Conclusions. The inclusion of lycopene or tomato extract in food for the organisms used is not recommended to improve pigmentation, but further studies are needed to demonstrate antioxidant effect.

  10. Acute ammonia toxicity in crucian carp Carassius auratus and effects of taurine on hyperammonemia.

    Science.gov (United States)

    Ren, Qianyan; Li, Ming; Yuan, Lixia; Song, Meize; Xing, Xiaodan; Shi, Ge; Meng, Fanxing; Wang, Rixin

    2016-12-01

    The four experimental groups were carried out to test the response of crucian carp Carassius auratus to ammonia toxicity and taurine: group 1 was injected with NaCl, group 2 was injected with ammonium acetate, group 3 was injected with ammonium acetate and taurine, and group 4 was injected with taurine. Fish in group 2 had the highest ammonia and glutamine contents, and the lowest glutamate content in liver and brain. Serum superoxide dismutase (SOD), glutathione (GSH) activities, red cell count (RBC), white cell count (WBC), lysozyme (LYZ) activity, complement C3 content of fish in group 2 reflected the lowest, but malondialdehyde content was the highest. Importantly, serum SOD and GSH activites, RBC, WBC, and LYZ activity, C3, C4 and total immunoglobulin contents of fish in group 3 were significantly higher than those of fish in group 2. This study indicates that ammonia exerts its toxic effects by interfering with amino acid transport, inducing ROS generation, leading to malondialdehyde accumulation and immunosuppression of crucian carp. The exogenous taurine could mitigate the adverse effect of high ammonia level on fish physiological disorder. Copyright © 2016 Elsevier Inc. All rights reserved.

  11. Persistence of experimental Rocio virus infection in the golden hamster (Mesocricetus auratus

    Directory of Open Access Journals (Sweden)

    Daniele Freitas Henriques

    2012-08-01

    Full Text Available Rocio virus (ROCV is an encephalitic flavivirus endemic to Brazil. Experimental flavivirus infections have previously demonstrated a persistent infection and, in this study, we investigated the persistence of ROCV infection in golden hamsters (Mesocricetus auratus. The hamsters were infected intraperitoneally with 9.8 LD50/0.02 mL of ROCV and later anaesthetised and sacrificed at various time points over a 120-day period to collect of blood, urine and organ samples. The viral titres were quantified by real-time-polymerase chain reaction (qRT-PCR. The specimens were used to infect Vero cells and ROCV antigens in the cells were detected by immunefluorescence assay. The levels of antibodies were determined by the haemagglutination inhibition technique. A histopathological examination was performed on the tissues by staining with haematoxylin-eosin and detecting viral antigens by immunohistochemistry (IHC. ROCV induced a strong immune response and was pathogenic in hamsters through neuroinvasion. ROCV was recovered from Vero cells exposed to samples from the viscera, brain, blood, serum and urine and was detected by qRT-PCR in the brain, liver and blood for three months after infection. ROCV induced histopathological changes and the expression of viral antigens, which were detected by IHC in the liver, kidney, lung and brain up to four months after infection. These findings show that ROCV is pathogenic to golden hamsters and has the capacity to cause persistent infection in animals after intraperitoneal infection.

  12. Cyprinid herpesvirus 2 infection emerged in cultured gibel carp, Carassius auratus gibelio in China.

    Science.gov (United States)

    Xu, Jin; Zeng, Lingbing; Zhang, Hui; Zhou, Yong; Ma, Jie; Fan, Yuding

    2013-09-27

    An epizootic with severe mortality has emerged in cultured gibel carp, Carassius auratus gibelio, in China since 2009, and caused huge economic loss. The signs and epidemiology background of the disease were investigated. Parasite examination, bacteria and virus isolation were carried out for pathogen isolation. The causative pathogen was obtained and identified as Cyprinid herpesvirus 2 (CyHV-2) by experimental infection, electron microscopy, cell culture, PCR assay and sequence alignment, designated as CyHV-2-JSSY. Experimental infection proved the high virulence of CyHV-2-JSSY to healthy gibel carp. Electron microscopy revealed that the viral nucleocapsid was hexagonal in shape measuring 110-120 nm in diameter with a 170-200 nm envelope. The virus caused significant CPE in Koi-Fin cells at the early passages, but not beyond the fifth passages. Sequence alignment of the partial viral helicase gene (JX566884) showed that it shared 99-100% identity to the published sequences of other CyHV-2 isolates. This study represented the first isolation and identification of CyHV-2 in cultured gibel carp in China and laid a foundation for the further studies of the disease. Copyright © 2013 Elsevier B.V. All rights reserved.

  13. Argon laser-induced damage in the goldfish (C. auratus) retina following whole-body hyperthermia

    Science.gov (United States)

    Deaton, Michael A.; Lund, David J.; Schuschereba, Steven T.; Dahlberg, Ann M.; Cowan, Beth L.; Lester, Paul; Odom, Daniel G.

    1990-07-01

    The heat shock response is a phenomenon common to all cells and is characterized by an increase in the rate of synthesis of intracellular heat shock proteins (HSPs) . The response occurs following rapid transient increases in terrerature sufficient to cause stress but not cell death. HSPs appear to perform protective functions that raise the cell''s tolerance to diverse noxious stimuli. Thus we postulated that we could limit laser-induced retinal darriage through induction of the heat shock -response. Corrmon goldfish (C. auratus) made hyperthermic by immersion in 35C water for 15 minutes and radiolabeled with [355]methionine showed retinal liSPs with apparent molecular weights of 110 90 70 and 35 kilodaltons. To test the protective effects of HSPs against laser injury goldfish were made hyperthermic and 4 and 24 hr later their retinas were irradiated with argon laser light (51 4 . 5 nm spot size at the cornea 3. 0 mm irradiance 125 mW/cm2) . NonhyperLhermic animals served as controls. Following 24 hr of recovery fish were terminated and retinas fixed for histology. Fundus photographs were taken irrunediately after laser exposure . Lesion diameters were measured from fundus photographs and evaluated statistically. The mean retinal lesion diameters of fish not subjected to hyperthermJ. a laser exposed 4 hr post hyperLhermia and laser exposed 24 hr post hyperthermia were 10. 25 1. 4 SD 8. 82 2. 1 SD and 6. 78

  14. Repair of UVC induced DNA lesions in erythrocytes from Carassius auratus gibelio

    International Nuclear Information System (INIS)

    Bagdonas, E.; Zukas, K.

    2004-01-01

    The kinetics of UVC (254 nm) irradiation induced DNA single-strand breaks generated during the excision repair of UV induced DNA damage in erythrocytes from Carassius auratus gibelio were studied using alkaline comet assay. Nucleotide excision repair recognised DNA lesions such as UVC induced cyclobutane pyrimidine dimers and 6-4 pyrimidine-pyrimidone photoproducts and produced DNA single-stranded breaks that were easily detected by comet assay. After irradiation of erythrocytes with 58 j/m 2 UVC dose, there was an increase in comet tail moment (CTM) at 2 hours post-radiation, whereas at 4 hours post-radiation CTM decreased and did not differ significantly from the control level (P=0,127). When erythrocytes were exposed to 173 J/m 2 UVC dose, the excision repair delayed in the beginning (0 hours), reached maximum level at 2 hours post-radiation (CTM-54,8) and showed slightly decreased level at 4 hours post-radiation (CTM=18,5). (author)

  15. Benzocaína e eugenol como anestésicos para o quinguio (Carassius auratus

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    F. Bittencourt

    2012-12-01

    Full Text Available Avaliaram-se os tempos de indução e recuperação de quinguios (Carassius auratus expostos a dois anestésicos, eugenol e benzocaína. Foram utilizados 128 juvenis com peso médio de 2,07±0,53g e comprimento total médio de 5,51±0,56cm. A benzocaína mostrou ser mais eficiente do que o eugenol em relação ao tempo, tanto para indução ao coma quanto para a recuperação à fuga e também no que diz respeito à sobrevivência. As doses de benzocaína com melhores resultados foram de 87,5 e 100mg.L-1. O eugenol proporcionou demora na indução e na recuperação dos animais, além de ter apresentado mortalidades quando as doses anestésicas foram elevadas.

  16. TINGKAH LAKU MAKAN LUTUNG JAWA Trachypithecus auratus DI KAWASAN PANCURAN 7 BATURADEN GUNUNG SLAMET JAWA TENGAH

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    Dwi Eliana

    2017-06-01

    Full Text Available Javan Langur (Trachypithecus auratus has an important ecological role in conserving the forest as their habitat by spreading seeds of fruits of their food. They are also responsible for the pattern of the plant diversity and forest regeneration as their habitat. Unfortunately, Javan Langur has been listed on Appendix II of CITES and categorized as vulnerable by the IUCN. Pancuran 7 Baturaden on the Mount Slamet slope is one of the few Javan Langur habitats in Java. Information on the Javan Langur in this area was inadequate. Therefore this study was aimed to get information on the type of food and the feeding behavior of Javan Langur, particularly in Pancuran 7 Baturaden. This study used a survey method, and the Animal Scan sampling technique was applied. The variables observed were sex ratio, population size, eating position, eating process, feeding locations, feeding duration, including the food type of the Javan Langur. Observed data for each variable were analyzed descriptively as percentages, and the results showed the Javan Langur were eating by sitting and standing, were marking trees to feed, were using hands and mouth to eat, were eating leaves and fruit, were eating on the trees and bushes. There were nine plant species as their food variety, while Matoa fruit and leave (Pometia  pinnata were their preferred foods.

  17. Characterization of actions of dopamine in the pituitary of the goldfish, Carassius auratus

    Energy Technology Data Exchange (ETDEWEB)

    Omeljaniuk, R.J.

    1988-01-01

    The dopamine receptor in the goldfish (Carassius auratus) pituitary and its involvement with inhibition of gonadotropin (GtH) and {alpha}-melanocyte stimulating hormone ({alpha}-MSH) release was studied. In vitro dopamine, in a dose-related manner, inhibited spontaneous GtH and {alpha}-MSH release from superfused fragments of pars distalis (PD) and neurointermediate lob (NIL), respectively; dopamine also inhibited sGnRH-A stimulation of GtH release. Thyrotropin releasing-hormone (TRH), in a dose-related manner, stimulated {alpha}-MSH release from NIL fragments; dopamine inhibited TRH action. The stereoisomers of apomorphine were equivalent in inhibiting GtH and {alpha}-MSH release from fragments treated with releasing factors. Domperidone, in a dose-related manner, antagonized dopamine action. ({sup 3}H)-Spiperone was used to radiolabel the goldfish pituitary dopamine receptor in vitro. The binding of ({sup 3}H)-spiperone had the characteristics of a receptor: tissue specificity, dependence on tissue quantity, reversibility, saturability, displaceability, specificity of binding with various drugs and a correlation of binding with biological effects were demonstrated. This is a low-affinity, high-capacity receptor which does not show binding stereoselectivity for apomorphine; domperidone binds avidly to this receptor. The NIL contains significantly greater numbers of this receptor compared to the PD.

  18. Fishery biology of the goldfish Carassius auratus (Linnaeus, 1758 in Lake Trasimeno (Umbria, Italy

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    Lorenzoni M.

    2010-01-01

    Full Text Available The fishery biology of the goldfish Carassius auratus was studied in Lake Trasimeno by analysing the selectivity of the equipment that can be used to control this invasive species. Sampling was conducted monthly from February 2003 to January 2004 by means of electrofishing, fyke-nets and gill-nets of different-sized mesh (22, 25, 28, 35, 40, 50, 70 and 80 mm. The average value of the CPUEs of goldfish caught was 10175.48 g·10−2·m−2 for gill-nets, 246.97 g·h−1 for fyke-nets and 606.50 g·min−1 for electrofishing. The efficiency of electrofishing was not uniform in all periods of the year, the mean CPUEs being highest in spring, when specimens of reproductive age were preferentially caught. Gill-nets yielded abundant catches of goldfish in all seasons, and displayed good sampling efficiency even in winter. With regard to the selectivity of gill-nets, estimated optimum selection lengths for each mesh size were: 11.92 cm, 13.55 cm, 15.18 cm, 18.97 cm, 21.68 cm, 27.10 cm, 37.94 cm and 43.36 cm. The results of this research demonstrate that the various fishing techniques can be effectively combined with the aim of controlling the goldfish population in Lake Trasimeno.

  19. Mathematical modelling of nutrient balance of a goldfish (Carassius auratus Linn. recirculating aquaculture system (GRAS

    Directory of Open Access Journals (Sweden)

    Sudeep Puthravilakom Sadasivan Nair

    2010-08-01

    Full Text Available In the present study, a goldfish (Carassius auratus Linn. recirculating aquaculture system (GRAS has been developed. The GRAS consisted of a culture tank, a screen filter and a foam fractionator for removal of particulate and dissolved solids and a trickling filter for conversion of ammonium- and nitrite-nitrogen to relatively harmless nitrate-nitrogen. The culture of goldfish at a stocking density of 1.08 kg/m3 was continued for a period of two and half months. Based on mass balance analysis of ammonium- and nitrate-nitrogen and assuming the trickling filter to be a plug flow reactor, a model was formulated to determine the necessary recirculation flow rate at different times of culture for maintaining the major nutrients, viz., ammonium- and nitrate-nitrogen below their permissible limits. The model was calibrated and validated using the real time data obtained from the experimental run. The high values of coefficient of determination and low values of root mean square error show the effectiveness of the model.

  20. Comparative aspects of calcium dynamics in calcified tissues in the goldfish Carassius auratus

    Energy Technology Data Exchange (ETDEWEB)

    Ichii, Taro; Mugiya, Yasuo (Hokkaido Univ., Hakodate (Japan). Faculty of Fisheries)

    1983-07-01

    The comparative nature of calcium physiology in bone, scales and otoliths was studied in young goldfish, Carassius auratus. Net calcium uptake by the fish was estimated to be 143 ..mu..g/g body weight/day. Of that, 79 % was distributed in bone, 13 % in scales, 3.5 % in otoliths and 4.5 % in soft tissues. Scales showed the highest incorporation of /sup 45/Ca per mg-tissue weight after 1 or 2 days in /sup 45/Ca-containing water; bone came second and otoliths last. However, 35 days after transfer to non-radioactive water, the order of descending radioactivity had changed to otoliths, bone and scales, reflecting different rates of calcium turnover. In bone, prelabeled /sup 45/Ca activity increased for the first 2 days after transfer and then decreased gradually (biological half-life, Tsub(0.5) = 94 days). In otoliths, prelabeled radioactivity consistently increased for 35 days. Scales showed two phases of calcium turnover. They lost about 33 % of their prelabeled radioactivity during the first 7 days (Tsub(0.5) = 10.5 days) in non-radioactive water, but thereafter the rate of decrease slowed down greatly (Tsub(0.5) = 210 days). These two phases of calcium turnover were found in the osseous layer (including calcium crystals in the fibrillary plate) of scales, indicating the presence of physiologically labile as well as stable forms of calcium in the layer.

  1. Fluoxetine and diclofenac interaction on food intake in goldfish, Carassius auratus

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    Mohammad Navid Forsatkar

    2014-07-01

    Full Text Available This study was carried out to investigate the interaction of simultaneous use of fluoxetine (Flx, a selective serotonin reuptake inhibitor, and diclofenac (Dcf, a nonsteroidal anti-inflammatory drug, on food intake in goldfish, Carassius auratus. Treatments with different dosage of Flx including control, 0, 1, and 10  µg/g body weight (BW were injected in the fish with mean weight of 30.16 ± 8.57 g every other day in total of 5 times. Then fish were exposed to 3 different levels of Dcf including 0, 10, and 100 mg/l for 5 days. Injection of fluoxetine significantly decreased food intake and consequently body weight. After 5 days exposure to Dcf, the amount of food intake in the Dcf receiving treatments of 1 mg/l and 10 mg/l was significantly larger than that of 0 mg/l Dcf recieving treatment in both the Flx dosage groups of 1 μg/g BW and 10 μg/g BW. Our results indicated that Dcf inhibits behavioral change effects of Flx showing the complex effects of pharmaceuticals on fish.

  2. p53 dependent apoptotic cell death induces embryonic malformation in Carassius auratus under chronic hypoxia.

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    Paramita Banerjee Sawant

    Full Text Available Hypoxia is a global phenomenon affecting recruitment as well as the embryonic development of aquatic fauna. The present study depicts hypoxia induced disruption of the intrinsic pathway of programmed cell death (PCD, leading to embryonic malformation in the goldfish, Carrasius auratus. Constant hypoxia induced the early expression of pro-apoptotic/tumor suppressor p53 and concomitant expression of the cell death molecule, caspase-3, leading to high level of DNA damage and cell death in hypoxic embryos, as compared to normoxic ones. As a result, the former showed delayed 4 and 64 celled stages and a delay in appearance of epiboly stage. Expression of p53 efficiently switched off expression of the anti-apoptotic Bcl-2 during the initial 12 hours post fertilization (hpf and caused embryonic cell death. However, after 12 hours, simultaneous downregulation of p53 and Caspase-3 and exponential increase of Bcl-2, caused uncontrolled cell proliferation and prevented essential programmed cell death (PCD, ultimately resulting in significant (p<0.05 embryonic malformation up to 144 hpf. Evidences suggest that uncontrolled cell proliferation after 12 hpf may have been due to downregulation of p53 abundance, which in turn has an influence on upregulation of anti-apoptotic Bcl-2. Therefore, we have been able to show for the first time and propose that hypoxia induced downregulation of p53 beyond 12 hpf, disrupts PCD and leads to failure in normal differentiation, causing malformation in gold fish embryos.

  3. Efeito da densidade de estocagem do quinguio, Carassius auratus L., 1758 (Osteichthyes, Cyprinidae, em suas fases iniciais de desenvolvimento Stocking density effect on goldfish, Carassius auratus L., 1758 (Osteichthyes, Cyprinidae fry, during its initial development phases

    Directory of Open Access Journals (Sweden)

    Christiano Rodrigues Schamber

    2002-05-01

    Full Text Available Objetivou-se avaliar a influência da densidade de estocagem sobre o desenvolvimento do quinguio, Carassius auratus (Osteichthyes, Cyprinidae em suas fases iniciais. Foram utilizados 300 larvas (Lt = 7,00 mm, distribuídas em 20 aquários (12-L. O delineamento experimental utilizado foi o inteiramente casualizado com quatro tratamentos (0,50; 1,00; 1,50 e 2,00 larvas/L e cinco repetições. A alimentação utilizada foi à base de náuplios de Artemia sp. (fornecida de forma proporcional a densidade e ração com 40% de proteína bruta (fornecida à vontade. Observou-se aumento linear (p0,001 pelas diferentes densidades de estocagem utilizadas. Os valores dos parâmetros físico-químicos permaneceram nos níveis adequados para o cultivo de peixes, embora os valores de pH tenham apresentado redução linear (pThis experiment aimed to evaluate the influence of stocking density on the goldfish, Carassius auratus (Osteichthyes, Cyprinidae development, during its initial phases. 300 fry (Lt.: 7.00 mm were distributed in 20 12-liter aquariums. The experimental design was entirely randomized, with four treatments (0.50; 1.00; 1.50 and 2.00 fry/liter and five replications. Food consisted of Artemia sp nauplii (supplied proportionally to density and rations with 40% raw protein, given ad libitum. While the biomass per aquarium linear increase (p0.001. On the other hand, quadract effect (p 0.001 by the different stocking densities. The physical-chemical parameters rates remained at adequate levels for fish culture, although pH values suffered linear decrease (pCarassius auratus fry culture can use such density during its initial development phases.

  4. Fathead minnows (Pimephales promelas) and goldfish (Carassius auratus) as standard fish in bioassays and their reaction to potential reference toxicants

    Energy Technology Data Exchange (ETDEWEB)

    Adelman, I.R.; Smith, L.L. Jr.

    1976-02-01

    Fathead minnows (Pimephales promelas) and goldfish (Carassius auratus) were compared for their suitability as standard bioassay fish. Both species showed the same variability of bioassay results when tested with four toxicants. Fathead minnows are recommended on the basis of their small size and on their capability for use in complete life cycle tests. On the basis of minimum variability of bioassay results, sodium chloride was superior for use as a reference toxicant. Both sodium chloride and pentachlorophenol seemed capable of detecting abnormal fish. On the basis of seven listed criteria either sodium chloride or pentachlorophenol would be acceptable as a reference toxicant.

  5. Temporal and spatial comparisons of the reproductive biology of northern Gulf of Mexico (USA) red snapper (Lutjanus campechanus) collected a decade apart.

    Science.gov (United States)

    Kulaw, Dannielle H; Cowan, James H; Jackson, Melissa W

    2017-01-01

    In studies done a decade apart, we provide evidence of a recent shift toward a slower progression to sexual maturity as well as reduced egg production, especially among young, small female red snapper, in the Gulf of Mexico (Gulf). Slower maturation rates (among fish ≤6 years old), lower GSI values and decreased spawning frequency were observed, and were especially pronounced in the northwestern Gulf. Furthermore, an Index of Reproductive Importance showed that young fish (ages 2-7) are contributing far less to the spawning stock in recent years, while older fish (>8 years) are contributing more, when compared to fish from the same age groups sampled in the previous decade. Coincident with these changes in reproductive output, fishing pressure has steadily declined gulf-wide, and spawning stock biomass and spawning potential ratio have increased. Thus, it is possible that the age structure of the red snapper stock is becoming less truncated, or that reproductive effort observed is due to the temporary influence of recent strong year classes produced in 2004 and 2006 as they begin to reach full reproductive potential. If the latter is true, careful documentation of the stock's reproductive dynamics during a time of population growth provides new understanding at the meta-population spatial and decadal temporal scales. In contrast, if the former is true, a truncated age structure due to overharvest can limit the productivity of the Gulf red snapper stock. In addition, we have learned that red snapper females in the northwestern Gulf collected on natural reefs and banks have much higher reproductive output than those on artificial reefs in the form of standing and toppled oil and gas platforms, thus making the need to know the relative abundance of females found on these disparate habitats an important next step toward better-understanding factors impacting the reproductive dynamics of this species.

  6. Diversity, occurrence and socio-economic aspects of snappers and job fish (Family: Lutjanidae) fisheries from Gulf of Mannar region, south-east coast of India.

    Digital Repository Service at National Institute of Oceanography (India)

    Murugan, A.; Vinod, K.; Saravanan, K.R.; Anbalagan, T.; Saravanan, R.; Sanaye, S.V.; Mojjada, S.K.; Rajagopal, S.; Balasubramanian, T.

    Journal of Geo-Marine Sciences Vol. 43 (4), April 2014, pp. 618-633 Diversity, occurrence and socio-economic aspects of snappers and job fish (Family: Lutjanidae) fisheries from Gulf of Mannar region, south-east coast of India A. Murugan...] Introduction The role of human activity on marine ecosystems is receiving increased attention with the perception that human activities are causing considerable economic loss for local fishing communities1. In particular, the increase in fishing pressure...

  7. Effect of ice storage on muscle protein properties and qualities of emulsion fish sausage from bigeye snapper (Priacanthus tayenus and lizardfish (Saurida undosquamis

    Directory of Open Access Journals (Sweden)

    Vittayanont, M.

    2005-01-01

    Full Text Available The chemical changes in fish muscle and natural actomyosin (NAM from bigeye snapper (Priacanthus tayenus and lizardfish (Saurida undosquamis muscle during 0, 2, 4, 6, 8, 10, 12 and 14 days of iced storage were studied. Myosin heavy chain (MHC of NAM extracted from two fish species was degraded throughout iced storage. However, no changes in actin were observed. The total volatile base (TVB. trimethylamine (TMA and surface hydrophobicity increased, while the total sulfhydryl content and emulsion capacity of NAM from both fish species decreased significantly as the storage time increased (p<0.05. A Texture ProfileAnalysis (TPA and shear force of emulsion fish sausages prepared from two fish species kept in ice for 0, 4, 8 and 12 days were investigated. The results showed that hardness, cohesiveness, gumminess, chewiness and shear force of sausage prepared from fish kept in ice were lower than those produced from fresh fish. However, no significant differences in adhesiveness were observed. Cooking loss of emulsion fish sausage from two fish species increased throughout storage time (p<0.05. The texture of bigeye snapper sausage was better than that of lizardfish sausages. Scanning electron microscopy (SEM micrographs of emulsion fish sausage from two fish species revealed bigger voids, thicker strands and less continuity of protein strands with increasing storage time. More microstructural changes were observed in sausages from lizardfish, compared to those in sausages from bigeye snapper.

  8. Effects of carbofuran, diuron, and nicosulfuron on acetylcholinesterase activity in goldfish (Carassius auratus).

    Science.gov (United States)

    Bretaud, S; Toutant, J P; Saglio, P

    2000-10-01

    Juvenile goldfish (Carassius auratus) were exposed to three widely used pesticides; carbofuran, diuron, and nicosulfuron. Acetylcholinesterase (AChE) activity and molecular forms of AChE were first characterized in brain and skeletal muscle of unexposed fish. Skeletal muscle had higher AChE activity than brain (306 and 215 nmol/min/mg protein, respectively). In brain, four molecular forms of AChE were found: A12, G4, G2, and G1. In the muscle, three molecular forms were found A12, A8, and G2. AChE activity was then evaluated in both tissues of fish exposed to different concentration of pesticides (5, 50, and 500 microg/L) for 6, 12, 24, and 48 h. In brain, AChE activity was significantly inhibited during all the periods of exposure in response to 50 microg/L (19-28%) and 500 microg/L (85-87%) carbofuran. Such effect was observed in the muscle only at 500 microg/L (86-92%). Carbofuran had no effect on the distribution of molecular forms. Significant inhibitions (9-12%) of brain AChE activity were also observed in response to diuron and nicosulfuron at 500 microg/L during all periods of exposure and for 50 microg/L nicosulfuron after 24 and 48 h. This study pointed out short-term effects of exposure to sublethal concentrations of the three pesticides, ranging among different chemical families, on brain and muscle AChE in goldfish. Copyright 2000 Academic Press.

  9. Reproductive activity of the frog Dendrobates auratus Girard in captivity breeding

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    Romana Křížová

    2010-01-01

    Full Text Available Research of the reproduction activities of Dendrobates auratus Girard, 1855 is part of a broader ana­ly­sis of the problems encountered when keeping frogs from the family Dendrobatidae in captivity. The aim is to achieve an optimization of measures for successful breeding of these amphibians, for both the amateur hobbyist and conservationist alike. Due to the wide area they have colonized, many colour variations exist; the reproduction parameters of six differently coloured examples of the species were tracked. The collection of data happened in standardized conditions over two consecutive years (2007, 2008 and was aimed at measuring five indicators concerning reproduction, understanding of egg-laying dynamics during a year, measuring the risk of stock loss during different stages of development and determining if any differences existed between the different colour variations in these parameters. A noticable alternating cycle of rest and reproduction activities has been observed between equinoxes. In the harvest there were usually 5–10 eggs with an average incubation period of 10 days (8–18 at a temperature of 22 °C. The total duration of development was on average 74 days (64–103. The number of eggs in a harvest and the duration of incubation were statistically dif­fe­rent with the blue form between different observed periods. Total mortality rate during ontogenesis reached 10–53%, the highest losses were in the period when the tadpole left its egg and began developing its hind legs, 5–60%. The optimisation of breeding conditions has been reccommended. Secondary be­ne­fits of this study research was the collection of new knowledge about ethology of this species in captivity.

  10. Uptake and accumulation of mercury from dental amalgam in the common goldfish, Carassius auratus

    International Nuclear Information System (INIS)

    Kennedy, C.J.

    2003-01-01

    Exposure of fish to concentrations of dental amalgam typically found in waste discharge leads to mercury accumulation in tissues. - In this study, the bioavailability and accumulation of mercury from external environmental exposure to mixed, cured, milled, sieved and proportioned dental amalgam was examined in the common goldfish, Carassius auratus. Fish were exposed to dental amalgam (particle size range from <0.10 to 3.15 mm) in order to represent the particle size and distribution of that found within the typical dental office wastewater discharge stream. Experimental amalgam water loadings were 0 g/l, 0.5 g/l and 1 g/l in glass aquaria at 15 deg. C for 28 days. Fish tissues were sampled at 5 min and 28 days of exposure, and the liver, brain, muscle and whole body analyzed for total mercury using cold vapor atomic fluorescence spectroscopy. Mercury was found in several tissues examined and generally increased with exposure to higher amounts of dental amalgam. The highest levels were found in the whole body (17.68±5.73 μg/g) followed by the liver (0.80±0.16 μg/g) and muscle (0.47±0.16 μg/g). The lowest concentrations were seen in the brain (0.28±0.19 μg/g). Compared to controls, concentrations in the whole body, muscle and liver in fish exposed for 28 days to the highest concentration of amalgam were 200-, 233-, and 40-fold higher, respectively. This study shows that mercury from an environmental exposure to representative samples of dental amalgam typically found within the dental wastewater discharge stream is bioavailable to fish and may accumulate in internal tissues

  11. Skin healing and scale regeneration in fed and unfed sea bream, Sparus auratus

    Directory of Open Access Journals (Sweden)

    Canario Adelino VM

    2011-10-01

    Full Text Available Abstract Background Fish scales are an important reservoir of calcium and phosphorus and together with the skin function as an integrated barrier against environmental changes and external aggressors. Histological studies have revealed that the skin and scales regenerate rapidly in fish when they are lost or damaged. In the present manuscript the histological and molecular changes underlying skin and scale regeneration in fed and fasted sea bream (Sparus auratus were studied using a microarray 3 and 7 days after scale removal to provide a comprehensive molecular understanding of the early stages of these processes. Results Histological analysis of skin/scales revealed 3 days after scale removal re-epithelisation and formation of the scale pocket had occurred and 53 and 109 genes showed significant up or down-regulation, respectively. Genes significantly up-regulated were involved in cell cycle regulation, cell proliferation and adhesion, immune response and antioxidant activities. 7 days after scale removal a thin regenerated scale was visible and only minor changes in gene expression occurred. In animals that were fasted to deplete mineral availability the expression profiles centred on maintaining energy homeostasis. The utilisation of fasting as a treatment emphasised the competing whole animal physiological requirements with regard to barrier repair, infection control and energy homeostasis. Conclusions The identification of numerous genes involved in the mitotic checkpoint and cell proliferation indicate that the experimental procedure may be useful for understanding cell proliferation and control in vertebrates within the context of the whole animal physiology. In response to skin damage genes of immune surveillance were up-regulated along with others involved in tissue regeneration required to rapidly re-establish barrier function. Additionally, candidate fish genes were identified that may be involved in cytoskeletal re

  12. Effects of neutral phytase on growth performance and phosphorus utilization in crucian carp (Carassius auratus).

    Science.gov (United States)

    Nie, Xin-Zheng; Chen, Sha; Zhang, Xiao-Xu; Dai, Bin-Yang; Qian, Li-Chun

    A feeding trial was conducted for nine weeks to investigate the effects of partially replacing Ca(H 2 PO 4 ) 2 with neutral phytase on the growth performance, phosphorus utilization, nutrient digestibility, serum biochemical parameters, bone and carcass mineral composition, and digestive-enzyme-specific activity in crucian carp (Carassius auratus). The diets prepared with 0.8%, 0%, and 1.8% Ca(H 2 PO 4 ) 2 (1%=1 g/100 g) supplements were regarded as the P 1 E 0 , negative control (NC), and positive control (PC) groups, respectively; the other three experimental diets were prepared with the addition of 200, 300, and 500 U/kg of neutral phytase, respectively, based on the P 1 E 0 group. Three hundred and eighty-four fish ((1.50±0.01) g) were randomly distributed in the six treatments with four replicates each. The fish were initially fed with 2%-3% diets of their body weight per day, with feeding twice daily (08:00 and 16:00), under a 12-h light/12-h dark cycle at the temperature of (27.56±0.89) °C. The results showed that supplemental phytase at different levels in the diet improved the final body weight, average daily gain, feed conversion ratio, phosphorus utilization, and protein efficiency ratio of crucian carp (PPhytase supplementation increased the mineral content in serum (P), bone (P, Ca), and carcass (P, Ca, Zn, Na, and Mg) (Pphytase-supplemented diets (Pphytase improved the growth performance, phosphorus utilization as well as nutrient utilization in crucian carp, and it can be considered an important nutritional replacement for Ca(H 2 PO 4 ) 2 .

  13. Uptake and accumulation of mercury from dental amalgam in the common goldfish, Carassius auratus

    Energy Technology Data Exchange (ETDEWEB)

    Kennedy, C.J

    2003-03-01

    Exposure of fish to concentrations of dental amalgam typically found in waste discharge leads to mercury accumulation in tissues. - In this study, the bioavailability and accumulation of mercury from external environmental exposure to mixed, cured, milled, sieved and proportioned dental amalgam was examined in the common goldfish, Carassius auratus. Fish were exposed to dental amalgam (particle size range from <0.10 to 3.15 mm) in order to represent the particle size and distribution of that found within the typical dental office wastewater discharge stream. Experimental amalgam water loadings were 0 g/l, 0.5 g/l and 1 g/l in glass aquaria at 15 deg. C for 28 days. Fish tissues were sampled at 5 min and 28 days of exposure, and the liver, brain, muscle and whole body analyzed for total mercury using cold vapor atomic fluorescence spectroscopy. Mercury was found in several tissues examined and generally increased with exposure to higher amounts of dental amalgam. The highest levels were found in the whole body (17.68{+-}5.73 {mu}g/g) followed by the liver (0.80{+-}0.16 {mu}g/g) and muscle (0.47{+-}0.16 {mu}g/g). The lowest concentrations were seen in the brain (0.28{+-}0.19 {mu}g/g). Compared to controls, concentrations in the whole body, muscle and liver in fish exposed for 28 days to the highest concentration of amalgam were 200-, 233-, and 40-fold higher, respectively. This study shows that mercury from an environmental exposure to representative samples of dental amalgam typically found within the dental wastewater discharge stream is bioavailable to fish and may accumulate in internal tissues.

  14. Physiological and condition-related traits in the gynogenetic-sexual Carassius auratus complex: different investments promoting the coexistence of two reproductive forms?

    Czech Academy of Sciences Publication Activity Database

    Šimková, A.; Hyršl, P.; Halačka, Karel; Vetešník, Lukáš

    2015-01-01

    Roč. 15, č. 154 (2015), s. 154 ISSN 1471-2148 R&D Projects: GA ČR GAP505/12/0375 Institutional support: RVO:68081766 Keywords : Carassius auratus * gene expression * growth Subject RIV: EG - Zoology Impact factor: 3.406, year: 2015

  15. Probiotic potential of Bacillus velezensis JW: Antimicrobial activity against fish pathogenic bacteria and immune enhancement effects on Carassius auratus.

    Science.gov (United States)

    Yi, Yanglei; Zhang, Zhenhua; Zhao, Fan; Liu, Huan; Yu, Lijun; Zha, Jiwei; Wang, Gaoxue

    2018-04-24

    This study evaluated the probiotic potential of B. velezensis JW through experimental and genomic analysis approaches. Strain JW showed antimicrobial activity against a broad range of fish pathogenic bacteria including Aeromonas hydrophila, Aeromonas salmonicida, Lactococcus garvieae, Streptococcus agalactiae, and Vibrio Parahemolyticus. Fish (Carassius auratus) were fed with the diets containing 0 (control), 10 7 , and 10 9  cfu/g of B. velezensis JW for 4 weeks. Various immune parameters were examined at 1, 2, 3, and 4 weeks of post-feeding. Results showed that JW supplemented diets significantly increased acid phosphatase (ACP), alkaline phosphatase (AKP), and glutathione peroxidase (GSH-PX) activity. The mRNA expression of immune-related genes in the head kidney of C. auratus was measured. Among them, the interferon gamma gene (IFN- γ) and tumor necrosis factor-α (TNF-α) showed higher expression after 3 and 4 weeks of feeding (P velezensis JW has the potential to be developed as a probiotic agent in aquaculture. Copyright © 2018. Published by Elsevier Ltd.

  16. Phenotypic and molecular variation in the green and black poison-dart frog Dendrobates auratus (Anura: Dendrobatidae from Costa Rica

    Directory of Open Access Journals (Sweden)

    Lisa D Patrick

    2009-11-01

    Full Text Available The green and black poison-dart frog Dendrobates auratus exhibits high intraspecific variation in hue color and pattern throughout its range, making it a very popular species in the pet trade. We analyzed the correspondence between color variation and molecular variation of D. auratus from Costa Rica using RAPD analysis. Twenty-six random primers were analyzed for variation in 99 individuals from seven populations. Color pattern was scored from digital images of the dorsal and ventral views. In general, frogs from the Caribbean coast had significantly more light coloration than black color but cannot be grouped by population based only on hue pattern. Only 3 RAPD primers were found to be polymorphic, representing a total of 16 loci. Most of the molecular variation encountered here occurs within populations, thus making unclear the degree of population structure and differentiation. Further examination of COI mtDNA sequences from our samples also supports these results. Partial Mantel correlations suggested that the pattern of molecular variation is not congruent with the variation in color pattern in this species, an outcome that is discussed in terms of phenotypic evolution. Rev. Biol. Trop. 57 (Suppl. 1: 313-321. Epub 2009 November 30.

  17. In vitro and in vivo antiparasitic activity of Azadirachtin against Argulus spp. in Carassius auratus (Linn. 1758).

    Science.gov (United States)

    Kumar, Saurav; Raman, R P; Kumar, Kundan; Pandey, P K; Kumar, Neeraj; Mohanty, Snatashree; Kumar, Abhay

    2012-05-01

    Argulus is one of the most common and predominant ectoparasites which cause serious parasitic disease and is a potent carrier of viruses and bacteria in the ornamental fish industry. In recent years, organic (herbs)-based medicines are widely used to cure the disease, and neem (Sarbaroganibarini) medicine is very popular and effective throughout the world. The present study was conducted to find the effects of Azadirachtin against Argulus spp. on Carassius auratus under in vitro and in vivo conditions. The 96-h median lethal concentration (LC(50)) for Azadirachtin EC 25% against Carassius auratus was found to be 82.115 mg L(-1). The antiparasitic activity test under in vitro and in vivo was evaluated at 1 (T1), 5 (T2), 10 (T3), 15 (T4) and 20 mg L(-1) (T5) to treat Argulus for 3 h and 72 h, respectively. In vitro effect of Azadirachtin solution led to 100% mortality of Argulus at 20 and 15 mg L(-1) for 2.5 and 3 h, respectively. Whereas, under in vivo test, the 100% antiparasitic efficacy of Azadirachtin solution was found at 15 and 20 mg L(-1) for 72 and 48 h, respectively. The EC(50) for 48 h was 20 mg L(-1), and thus, therapeutic index is 4.10. The results provided evidence that Azadirachtin can be used as a potential agent for controlling Argulus.

  18. Electron paramagnetic resonance evidence of hydroxyl radical generation and oxidative damage induced by tetrabromobisphenol A in Carassius auratus

    Energy Technology Data Exchange (ETDEWEB)

    Shi Huahong [State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Nanjing 210093 (China)]. E-mail: huahongshi@tom.com; Wang Xiaorong [State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Nanjing 210093 (China); Luo Yi [State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Nanjing 210093 (China); Su Yan [State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Nanjing 210093 (China)

    2005-09-30

    Tetrabromobisphenol A (TBBPA) is one of the most widely used brominated flame retardants (BFRs). To confirm its putative oxidative stress-inducing activity, freshwater fish Carassius auratus were injected intraperitoneally with TBBPA. One experiment lasted 3 h to 28 days after a single injection of 100 mg/kg TBBPA, and the other lasted 24 h after a single injection of 0-300 mg/kg TBBPA. Reactive oxygen species (ROS) were trapped by phenyl-tert-butyl nitrone (PBN) and detected by electron paramagnetic resonance (EPR). Protein carbonyl (PCO) and lipid peroxidation product (LPO) content were also determined. A six-line EPR spectrum was detected in the sample prepared in air, and a multiple one was obtained in nitrogen. The observed spectrum in nitrogen fits the simulation one with PBN/{center_dot}OCH{sub 3} and PBN/{center_dot}CH{sub 3} quite well. As compared to the control group, TBBPA significantly induced ROS production marked by the intensity of the prominent spectra in liver and bile. TBBPA (100 mg/kg) also significantly increased PCO content in liver starting 24 h and LPO content 3 days after injection. Either PCO or LPO content showed significant relation with ROS production. Based on the hyperfine constants and shape of the spectrum, ROS induced by TBBPA was determined as {center_dot}OH. The results clearly indicated that TBBPA could induce {center_dot}OH generation and result in oxidative damage in liver of C. auratus.

  19. Reproductive biology of black seabream Acanthopagrus schlegelii, threadfin porgy Evynnis cardinalis and red pargo Pagrus major in the northern South China Sea with consideration of fishery status and management needs.

    Science.gov (United States)

    Law, C S W; Sadovy de Mitcheson, Y

    2017-07-01

    The reproductive biology of three commercially significant seabream species (family: Sparidae) Acanthopagrus schlegelii, Evynnis cardinalis and Pagrus major, taken from Hong Kong and adjacent northern South China Sea (SCS) waters, were investigated for their sexual patterns, spawning seasons, length at maturity and exploitation in relation to their conservation and management status. Histological analysis showed E. cardinalis and P. major to be functionally gonochoristic, the latter having a bisexual juvenile stage and being a rudimentary hermaphrodite. Acanthopagrus schlegelii is a protandric hermaphrodite. Standard length (L S ) at 50% sex change for A. schlegelii is 291 mm. L S at 50% female maturity for E. cardinalis and P. major are 117 and 332 mm, respectively. For all three species, the spawning period falls between November and March. The study highlights geographical differences in reproductive biology among the species and a paucity of fishery or other population-related data. While heavy fishing pressure, life-history characteristics and absence of effective management throughout the geographic ranges of these species make them susceptible to overfishing, they nonetheless appear to be generally more resilient than many other taxa that comprise the multi-species fisheries of the region, possibly due to their relatively rapid sexual maturation and spatial movement patterns. Overall, however, little is known of the biology, fishing history and current fishery status of sparids in general in the northern SCS and the current study is one of the first to examine such aspects of this family in the region and to consider appropriate management options. © 2017 The Fisheries Society of the British Isles.

  20. Induction of Fish Biomarkers by Synthetic-Based Drilling Muds

    Science.gov (United States)

    Gagnon, Marthe Monique; Bakhtyar, Sajida

    2013-01-01

    The study investigated the effects of chronic exposure of pink snapper (Pagrus auratus Forster), to synthetic based drilling muds (SBMs). Fish were exposed to three mud systems comprised of three different types of synthetic based fluids (SBFs): an ester (E), an isomerized olefin (IO) and linear alpha olefin (LAO). Condition factor (CF), liver somatic index (LSI), hepatic detoxification (EROD activity), biliary metabolites, DNA damage and stress proteins (HSP-70) were determined. Exposure to E caused biologically significant effects by increasing CF and LSI, and triggered biliary metabolite accumulation. While ester-based SBFs have a rapid biodegradation rate in the environment, they caused the most pronounced effects on fish health. IO induced EROD activity and biliary metabolites and LAO induced EROD activity and stress protein levels. The results demonstrate that while acute toxicity of SBMs is generally low, chronic exposure to weathering cutting piles has the potential to affect fish health. The study illustrates the advantages of the Western Australian government case-by-case approach to drilling fluid management, and highlights the importance of considering the receiving environment in the selection of SBMs. PMID:23894492

  1. Method to measure the force to pull and to break pin bones of fish.

    Science.gov (United States)

    Balaban, Murat O; Jie, Hubert; Yin Yee, Yin; Alçiçek, Zayde

    2015-02-01

    A texture measurement device was modified to measure the force required to pull pin bones from King salmon (Oncorhynchus tshawytscha), snapper (Pagrus auratus), and kahawai (Arripis trutta). Pulled bones were also subjected to tension to measure the breaking force. For all fish, the pulling force depended on the size of the fish, and on the length of the pin bone (P bones. For example, fresh small salmon (about 1500 g whole) required 600 g on average to pull pin bones, and large fish (about 3700 g whole) required 850 g. Longer bones required greater pulling force. The breaking force followed the same trend. In general, the breaking force was greater than the pulling force. This allows the removal of the bones without breaking them. There was no statistically significant (P > 0.05) difference between the forces (both pulling and breaking) from fresh and frozen/thawed samples, although in general frozen/thawed samples required less force to pull. With the quantification of pulling and breaking forces for pin bones, it is possible to design and build better, "more intelligent" pin bone removal equipment. © 2015 Institute of Food Technologists®

  2. Temporal Assessment of Natural Radionuclide Bioaccumulation by the Cubera Snapper Fish (Lutjanus Cyanopterus, Cuvier, 1828) from the Brazilian Coast

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, W. S. [Coordenacao de Protecao Radiologica, Unidade de Tratamento de Mineiros (UTM), Industrias Nucleares do Brasil (INB), Pocos de Caldas, MG and Curso de Pos-Graduacao em Biologia Marinha, Instituto de Biologia, Depto. de Biologia Marinha, Universidade Federal Fluminense UFF, Niteroi, RJ (Brazil); Kelecom, A. [Curso de Pos-Graduacao em Biologia Marinha, Instituto de Biologia, Depto. de Biologia Marinha, Universidade Federal Fluminense (UFF), and Programa de Pos-Graduacao em Ciencia Ambiental, Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil); Py, Jr., D. A. [Coordenacao de Protecao Radiologica, Unidade de Tratamento de Mineiros (UTM), Industrias Nucleares do Brasil (INB), Pocos de Caldas, MG (Brazil)

    2013-07-15

    The present study aims to assess the seasonal variation in the accumulation of the isotopes {sup 238}U, {sup 235}U, {sup 234}U, {sup 232}Th, {sup 230}Th, {sup 228}Th, {sup 226}Ra, {sup 228}Ra and {sup 210}Pb in the cubera snapper fish from the coast of the state of Ceara (Brazil). The fish was caught in January (six specimens), June (eight) and September (seven). The uranium and thorium were analysed by electrodeposition, followed by alpha spectrometry. The isotopes of radium and lead were selectively co-precipitated and measured by gross alpha radiometry for {sup 226}Ra and gross beta radiometry for {sup 228}Ra and {sup 210}Pb. No seasonal variations could be observed neither for the uranium and thorium isotopes nor for the {sup 226}Ra isotope. For {sup 228}Ra, the activity concentration in February was equal to that of September, and both were higher than in June. For {sup 210}Pb, the situation was different: the month of February showed a lower activity concentration than in June, which in turn was lower than in September. (author)

  3. Occurrence and intensity of parasites in goldfish (Carassius auratus L. from Guilan province fish ponds, north Iran

    Directory of Open Access Journals (Sweden)

    Roohi Javad Daghigh

    2016-03-01

    Full Text Available In this survey 109 specimens of goldfish (Carassius auratus were collected from Guilan fish ponds during 2012-13. After recording biometric characteristics, common parasitology methods were used. In the present study 11 parasite species were recovered from goldfish. Parasitofauna consisted of two protozoans: Ichthyophthirius multifiliis and Trichodina sp.; one digenean trematodes: Diplostomum spathaceum; six monogenean trematodes: Dactylogyrus vastator, Dactylogyrus formosus, Dactylogyrus baueri, Dactylogyrus anchoratus and Gyrodactylus sp.; one crustacean: copepodid stage of Lernaea cyprinacea and one nematodes larvae. All the monogeneans found during the current study are considered new locality records for goldfish in Guilan province, Iran. Mean intensity of infection and abundances of parasite species (with prevalences >10% among seasons were tested by the Kruskal-Wallis test (KW, multiple comparisons and Conover-Inman test. Results have shown that monogeneans had the highest prevalence values (49.54% in goldfish in Guilan fish ponds.

  4. Continuous flow bioassay method to evaluate the effects of outboard motor exhausts and selected aromatic toxicants on fish. [Carassius auratus

    Energy Technology Data Exchange (ETDEWEB)

    Brenniman, G. (Univ. of Illinois, Chicago); Hartung, R.; Weber, W.J. Jr.

    1976-01-01

    A continuous flow bioassay system was designed to measure the effects of outboard motor exhaust (OME) emissions and selected volatile and evaporative aromatic toxicants on goldfish (Carassius auratus). Continuous flow bioassays were run for 24, 48, 72, 96, and 720 h to determine lethal concentrations for 50 percent of individuals (LC 50's) for leaded OME, non-leaded OME, toluene, xylene, and 1,3,5 trimethylbenzene, the three individual compounds having been identified as significant aromatic components of OME. The 96 h LC-50's for these substances were found to be 171, 168, 23, 17, and 13 ppm, respectively. The values of 171 and 168 ppm for the two OME's are given in terms of gallons of fuel burned per million gallons of water. The continuous flow bioassay method was demonstrated to be a more reliable indicator of the effects of OME pollutants on aquatic organisms than is the static bioassay method.

  5. Lethal and mutagenic effects of radiation and chemicals on cultured fish cells derived the erythrophoroma of goldfish (Carassius auratus)

    Energy Technology Data Exchange (ETDEWEB)

    Mitani, H. (Tokyo Univ. (Japan). Inst. of Zoology)

    1983-01-01

    GEM 199 cells derived from an eryhtrophoroma of goldfish (Carassius auratus), which had a high plating efficiency, were used to investigate the lethal and mutational effects of radiations (UV and ..gamma..-rays) and chemicals (4NQO and MNNG). The cells were more resistant to rays than mammalian cells and CAF-MM1 cells derived from the normal fin tissue of goldfish. They were also more resistant to UV-irradiation than CAF-MM1 cells. Photoreactivation after UV-irradiation was present in GEM 199 cells for both survival and mutation. The initial shoulder of the survival curve of UV-irradiated cells was reduced greatly by caffeine, suggesting a high activity of the post-replication repair. The spontaneous mutation frequency to ouabain resistance was 1-5x10/sup -6/ clones per viable cell. MNNG was effective in inducing ouabain-resistant mutation, while 4NQO and ..gamma..-rays did not induce mutation.

  6. ZONASI PERIKANAN PASI UNTUK KEPENTINGAN PEMANFAATAN SECARA BERKELANJUTAN SUMBERDAYA IKAN KAKAP MERAH DI KEPULAUAN LEASE (Pasi Zone for Interest Sustainable Utilization of Red Snapper Resources in Lease Islands

    Directory of Open Access Journals (Sweden)

    Delly Dominggas Paulina Matrutty

    2015-01-01

    Full Text Available ABSTRAK Pasi adalah daerah penangkapan spesifik ikan kakap merah di Kepulauan Lease. Eksploitasi terhadap sumberdaya ikan kakap merah cenderung tinggi akhir-akhir ini karena sangat disukai di pasar lokal, regional maupun internasional, selain dijadikan sebagai objek wisata pancing. Kondisi ini akan mengancam kelestarian sumberdaya jika tidak dikelola dengan baik. Penetapan zonasi merupakan salah satu alternatif pengelolaan sumberdaya perikanan yang baik, dan jika dilakukan dengan benar akan memberikan nilai tambah bagi masyarakat, tanpa mengganggu kelestariannya. Tujuan penelitian ini adalah menentukan zonasi perikanan pasi untuk kepentingan pemanfaatan berkelanjutan sumberdaya ikan kakap merah di Kepulauan Lease. Data yang digunakan meliputi hasil kajian potensi sumberdaya ikan kakap merah, kondisi oseanografi  daerah penangkapan (pasi, dan sistem nilai perikanan pasi yang meliputi komponen nilai dasar ekologi, sosial dan teknologi, serta metode Participatory Rural Appraisal (PRA.  Berdasarkan kriteria yang dibangun dari seluruh aspek tersebut  maka dibuat zonasi khusus untuk kawasan pasi di Kepulauan Lease.  Berdasarkan hasil  analisis  diperoleh 4 (empat dari  25  pasi  ditetapkan sebagai zona lindung, 21 pasi ditetapkan sebagai zona perikanan berkelanjutan sub-zona perikanan tangkap, sedangkan 13 di antaranya ditetapkan sebagai zona pemanfaatan sub-zona wisata pancing. ABSTRACT Pasi is a specific fishing ground for Red Snapper in Lease Islands.  Recently, the exploitation of the red snapper population is tend to increase due to high demand of local, regional and international market as well as object for fishing tourism. This condition will threat the sustainability of the resources as if it is unmanaged in good way.  Zone determination is one of the alternatives of good fisheries resources management. If it is done in a right way, it will add value to community without disturbing the sustainability. The objectives of the present

  7. Uptake and release kinetics of 134Cs by goldfish (Carassius auratus) and 137Cs by zebra fish (Brachydanio rerio) in controlled aquatic environment

    International Nuclear Information System (INIS)

    Srivastava, A.; Reddy, S.J.; Kelber, O.; Urich, K.; Denschlag, H.O.

    1994-01-01

    The uptake and release kinetics of 134 Cs by Goldfish (Carassius auratus) and 137 Cs by Zebra Fish (Brachydanio rerio) from aquatic media of different ionic compositions and temperature was studied in controlled laboratory conditions. The accumulation of radiocesium in the case of Brachydanio rerio is observed to be strongly dependent on the potassium ion concentration of the aquatic medium, but in the case of Carassius auratus this dependence is quite weak. The biological half-lives of the cesium isotopes incorporated into the fish investigated in the present work vary from 19 to 80 days and are influenced by the temperature and the ionic composition of the aquatic medium. (author) 19 refs.; 1 fig.; 3 tabs

  8. The study of the peptide composition of the supernatants from mealworm Tenebrio molitor larvae and goldfish Carassius auratus during cold acclimation

    Directory of Open Access Journals (Sweden)

    А. К. Гулевский

    2016-11-01

    Full Text Available The molecular-mass distribution of peptides from supernatants, obtained from the tissues of larvae Tenebrio molitor and goldfish Carassius auratus during cold acclimation, has been determined by chromatography. The results showed that peptide spectrum of the supernatants from larvae T. molitor and C. auratus varied during cold acclimation. The supernatants from non-acclimated larvae of T. molitor and deacclimated fish possessed the highest number of peptide fractions. Furthermore, the cold-acclimated larvae of T. molitor had the peptide fractions of the low molecular weight (ca. 5.4×102 ÷22.6×102 Da, and non-acclimated insects had the peptides of the high molecular weight (ca. 46.8×102÷66×102 Da. Next, the organ-specific changes of the peptide composition of the goldfish during winter deacclimation have been revealed. Specifically, the low molecular weight peptides (ca. (14.1 ± 0.3×102 and (6.75 ± 0.25×102 Da, have been detected in the C. auratus muscles, and both the high (ca. (67.83 ± 0.21×102 ( ca. 64.16 ± 0.26×102 Da and low (ca. (34.1 ± 1.0×102 and (14.29 ± 0.15×102 Da molecular weight peptides have been detected in the liver. Quantitative and qualitative changes in the peptide spectra from supernatants of the T. molitor and C. auratus during cold acclimation could be one of the mechanisms of their natural adaptation to low temperatures.

  9. The effects of temperature on gastric emptying time of malabar blood snapper (Lutjanus malabaricus, Bloch & Schneider 1801) using X-radiography technique

    International Nuclear Information System (INIS)

    Mazumder, Sabuj Kanti; Ghaffar, Mazlan Abd.; Das, Simon Kumar

    2015-01-01

    Water temperature can influence the metabolic rate of ectothermal animal such as, fish. This study aims to investigate the gastric emptying time (GET) of malabar blood snapper (L. malabaricus) at different temperature (22, 26, 30, 34°C) fed with commercial diet pellet. The gastric emptying times at different temperatures were determined X-radiographically, using barium sulfate (BaSO 4 ) as a contrast medium food marker. The food marker and X-radiography showed that initial voidance of fecal matter began 4-8 h after feeding at all temperature. The fastest GET (18 h) was obsereved at 30°C, whereas the longest (28 h) GET was seen at 22°C. GET was increased with increasing temperature up to 30°C whereas decreased at extreme (34°C) and stress temperature (22°C). The findings of this study suggest to culture L. malabaricus at 26-30°C with commercial diet pellet as this temperature ranges escalate the faster digestion process which may contribute faster growth rate of this commercially important fish species. Overall, the data obtained from this study may have important consequences for optimization of commercial production of malabar blood snapper

  10. The effects of temperature on gastric emptying time of malabar blood snapper (Lutjanus malabaricus, Bloch & Schneider 1801) using X-radiography technique

    Energy Technology Data Exchange (ETDEWEB)

    Mazumder, Sabuj Kanti, E-mail: sabujsau@gmail.com [School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor (Malaysia); Ghaffar, Mazlan Abd., E-mail: magfish05@yahoo.com [Marine Ecosystem Research Centre (EKOMAR), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor (Malaysia); Institute of Tropical Aquaculture, University of Malaysia Terengganu, 21030 Kuala Terengganu (Malaysia); Das, Simon Kumar, E-mail: skdas-maa@yahoo.com [School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor (Malaysia); Marine Ecosystem Research Centre (EKOMAR), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor (Malaysia)

    2015-09-25

    Water temperature can influence the metabolic rate of ectothermal animal such as, fish. This study aims to investigate the gastric emptying time (GET) of malabar blood snapper (L. malabaricus) at different temperature (22, 26, 30, 34°C) fed with commercial diet pellet. The gastric emptying times at different temperatures were determined X-radiographically, using barium sulfate (BaSO{sub 4}) as a contrast medium food marker. The food marker and X-radiography showed that initial voidance of fecal matter began 4-8 h after feeding at all temperature. The fastest GET (18 h) was obsereved at 30°C, whereas the longest (28 h) GET was seen at 22°C. GET was increased with increasing temperature up to 30°C whereas decreased at extreme (34°C) and stress temperature (22°C). The findings of this study suggest to culture L. malabaricus at 26-30°C with commercial diet pellet as this temperature ranges escalate the faster digestion process which may contribute faster growth rate of this commercially important fish species. Overall, the data obtained from this study may have important consequences for optimization of commercial production of malabar blood snapper.

  11. Initial sexual maturity and fecundity of the goldfish Carassius auratus (Perciformes: Cyprynidae under semi-controlled conditions

    Directory of Open Access Journals (Sweden)

    A.A Ortega-Salas

    2006-12-01

    Full Text Available We studied the time of initial sexual maturity and the fecundity of the goldfish Carassius auratus under semi-controlled conditions. During the study the water temperature fluctuated from 21 to 30 ºC, the pH between 7 and 8, and the dissolved oxygen between 5.5 and 7 ppm. Sixty days old juvenile fish received prophylactic treatments of brackish water (15 ‰ and methylene blue baths (1 %, and they were fed with live Daphnia magna. The initial sexual maturity occurred between 225 and 233 days of captivity. Mean absolute fecundity was 2 347 (± 302.2 SD ova per gonad, and mean relative fecundity was 63.44 eggs/g fish weight. Gonadosomatic index was between 7.5 and 9.0. Our data will allow ornamental fish producers to calculate the time of initial maturity and fecundity for this species. Rev. Biol. Trop. 54 (4: 1113-1116. Epub 2006 Dec. 15.Estudiamos el tiempo inicial de madurez sexual y la fecundidad del pez dorado Carassius auratus en condiciones semi-controladas. Durante el estudio la temperatura del gua varió de 21 a 30 ºC, el pH entre 7 y 8, y el oxígeno disuelto entre 5.5 y 7 ppm. Jóvenes de 60 días de edad recibieron tratamiento profiláctico con agua salobre (15 ‰, y baños de azul de metileno (1 %. Se les alimentó con Daphnia magna. La madurez sexual inicial fue tras 225 a 233 días en cautiverio. La fecundidad absoluta media fue de 2 347 (±302.2 DS óvulos por gónada y la fecundidad relativa fue de 63.44 óvulos/g del peso del pez. El índice gonadosomático se calculó entre 7.5 and 9.0.

  12. Spatial and temporal variability in the otolith chemistry of the Brazilian snapper Lutjanus alexandrei from estuarine and coastal environments.

    Science.gov (United States)

    Aschenbrenner, A; Ferreira, B P; Rooker, J R

    2016-07-01

    Otolith chemistry of juvenile and adult individuals of the Brazilian snapper Lutjanus alexandrei was measured to assess the utility of natural markers for investigating individual movements. Individuals were collected over a 3-year period (2010-2012) along the north-eastern coast of Brazil from both estuarine (juvenile to sub-adult stages) and coastal (sub-adult to adult stages) areas. Six elements ((7) Li, (24) Mg, (55) Mn, (59) Co, (88) Sr and (137) Ba) were measured in sectioned otoliths of L. alexandrei using laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS). Edge composition analysis indicated that element:Ca ratios in the otoliths of juvenile and sub-adult L. alexandrei from estuaries were not significantly different among the three consecutive years (2010, 2011 and 2012), suggesting that physicochemical conditions within the nursery area investigated were temporally stable. Similarly, apart from two elements (Ba and Co), element:Ca ratios for larger L. alexandrei inhabiting coastal waters were also similar. In contrast, otolith chemistry of similar sized L. alexandrei from estuarine and coastal areas was significantly different (based on recently accreted material). Otolith Mn:Ca and Ba:Ca were both significantly higher for L. alexandrei collected in estuaries compared to fish from adjacent coastal reefs, while the opposite trend was observed for Sr:Ca. Given the pronounced differences in otolith chemistry between estuarine and coastal areas, element:Ca transects were constructed from the core to margin of the otoliths for adults (age 7+ years) collected on reefs to determine the timing of movement (ontogenetic migration) from estuarine to coastal areas. Based on observed patterns of decline for both Mn:Ca and Ba:Ca, it appears that L. alexandrei begin the move to more coastal habitats (i.e. lower element:Ca ratios) after age 2 years. The patterns observed for this species highlight the importance of conserving connectivity between

  13. THE EFFECTS OF DIFFERENT LEVELS OF DIETARY PROTEIN AND LIPID ON THE GROWTH OF RED SNAPPER, Lutjanus sebae

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    Nyoman Adiasmara Giri

    2009-06-01

    Full Text Available Red snapper, Lutjanus sebae is favored in mariculture activities because it has a relatively good market and price. Technology for big scale seed production of this species has been developed and is now adequate to supply seed for grow-out activities. However, the availability of artifical diets for L. sebae is still a major constraint for grow-out production. Data on optimum dietary protein and lipid requirements for this fish as a basic information in feed development is not available yet. The objective of the present study was to find out dietary protein and lipid requirements for juvenile of L. sebae. A 70-day feeding experiment was conducted in 24 fiberglass tanks, 200 L volume. Each tank was equipped with a flow-through water system. Twenty five hatchery-produced juveniles of L. sebae (43.1 g BW were randomly selected and stocked in each tank. The fish were fed with the experimental diets twice everyday at a level of 3% of biomass for the first 4 weeks, and then 2% of biomass afterward. Twelve experimental diets were prepared in form of dry pellet containing casein and fish meal as the main protein sources. Experimental diet had 4 levels of crude protein (32%, 37%, 42%, and 47% and each protein level consisted of 3 levels of lipid (7%, 12%, and 17%. The experiment employed factorial method with completely random design using 12 combination treatments and 2 replications for each treatment. Result of the experiment showed that there was no significant effect of dietary protein and lipid on growth, feed consumption, and feed efficiency of tested fish. Growth and feed efficiency of fish fed on diet containing 42% and 47% crude protein were significantly higher than that of fish fed on diet containing 32% and 37% crude protein. High lipid content in the diet (17% resulted in poor growth and poor feed efficiency. This data indicates that Lutjanus sebae has limited ability to utilize dietary lipid as an energy

  14. Effects of nutritional history on stress response in gibel carp (Carassius auratus gibelio) and largemouth bass (Micropterus salmoides).

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    Jiang, Danli; Wu, Yubo; Huang, Di; Ren, Xing; Wang, Yan

    2017-08-01

    The stress response of omnivorous gibel carp (Carassius auratus gibelio) and carnivorous largemouth bass (Micropterus salmoides) with different nutritional history were evaluated. A 2×2 layout, including two fish species (gibel carp or largemouth bass) and two nutritional history (fasted or fed to satiation for four weeks), was used. After feeding or fasting, the fishes were subjected to an acute handling. Fasting resulted in decrease of plasma glucose level and liver glycogen content of gibel carp and largemouth bass. After handling stress, plasma levels of cortisol, glucose and lactate of gibel carp and largemouth bass increased, regardless the fasted fish or fed fish. During the period from 0h to 24h post-stress, the fasted gibel carp exhibited lower plasma cortisol and glucose levels, brain and liver glycogen contents, and liver phosphoenolpyruvate carboxykinase (PEPCK) activity compared with the fed counterpart. The plasma glucose level, brain glucose level, brain and liver glycogen contents were lower, while the liver PEPCK and hexokinase (HK) activities were higher, in the faster largemouth bass than the fed counterpart. This study indicates that nutritional history can influence stress response of gibel carp and largemouth bass, and the stress response is less severe in the fasted fish relative to the fed counterpart. This study also reveals that gibel carp and largemouth bass may have different strategies in response to fasting and acute handling stress. Copyright © 2017 Elsevier Inc. All rights reserved.

  15. Standard metabolic rate predicts growth trajectory of juvenile Chinese crucian carp (Carassius auratus under changing food availability

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    Ling-Qing Zeng

    2017-09-01

    Full Text Available Phenotypic traits vary greatly within populations and can have a significant influence on aspects of performance. The present study aimed to investigate the effects of individual variation in standard metabolic rate (SMR on growth rate and tolerance to food deprivation in juvenile Chinese crucian carp (Carassius auratus under varying levels of food availability. To address this issue, 19 high and 16 low SMR individuals were randomly assigned to a satiation diet for 3 weeks, whereas another 20 high and 16 low SMR individuals were assigned to a restricted diet (approximately 50% of satiation for the same period. Then, all fish were completely food-deprived for another 3 weeks. High SMR individuals showed a higher growth rate when fed to satiation, but this advantage of SMR did not exist in food-restricted fish. This result was related to improved feeding efficiency with decreased food intake in low SMR individuals, due to their low food processing capacity and maintenance costs. High SMR individuals experienced more mass loss during food deprivation as compared to low SMR individuals. Our results here illustrate context-dependent costs and benefits of intraspecific variation in SMR whereby high SMR individuals show increased growth performance under high food availability but had a cost under stressful environments (i.e. food shortage.

  16. Effect of selenium nanoparticles with different sizes in primary cultured intestinal epithelial cells of crucian carp, Carassius auratus gibelio

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    Wang YB

    2013-10-01

    Full Text Available Yanbo Wang, Xuxia Yan, Linglin Fu Marine Resources and Nutrition Biology Research Center, Food Quality and Safety Department, Zhejiang Gongshang University, Hangzhou, People's Republic of China Abstract: Nano-selenium (Se, with its high bioavailability and low toxicity, has attracted wide attention for its potential application in the prevention of oxidative damage in animal tissues. However, the effect of nano-Se of different sizes on the intestinal epithelial cells of the crucian carp (Carassius auratus gibelio is poorly understood. Our study showed that different sizes and doses of nano-Se have varied effects on the cellular protein contents and the enzyme activities of secreted lactate dehydrogenase, intracellular sodium potassium adenosine triphosphatase, glutathione peroxidase, and superoxide dismutase. It was also indicated that nano-Se had a size-dependent effect on the primary intestinal epithelial cells of the crucian carp. Thus, these findings may bring us a step closer to understanding the size effect and the bioavailability of nano-Se on the intestinal tract of the crucian carp. Keywords: selenium nanoparticle, intestinal epithelial cell, crucian carp, primary culture

  17. Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)

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    Pang, Meixia; Luo, Weiwei; Yu, Xiaomu; Zhou, Ying; Tong, Jingou

    2018-01-01

    Feed efficiency is an economically crucial trait for cultured animals, however, progress has been scarcely made in the genetic analyses of feed conversion efficiency (FCE) in fish because of the difficulties in measurement of trait phenotypes. In the present investigation, we present the first application of RNA sequencing (RNA-Seq) combined with differentially expressed genes (DEGs) analysis for identification of functional determinants related to FCE at the gene level in an aquaculture fish, crucian carp (Carassius auratus). Brain tissues of six crucian carp with extreme FCE performances were subjected to transcriptome analysis. A total of 544,612 unigenes with a mean size of 644.38 bp were obtained from Low- and High-FCE groups, and 246 DEGs that may be involved in FCE traits were identified in these two groups. qPCR confirmed that genes previously identified as up- or down-regulated by RNA-Seq were effectively up- or down-regulated under the studied conditions. Thirteen key genes, whose functions are associated with metabolism (Dgkk, Mgst3 and Guk1b), signal transduction (Vdnccsa1b, Tgfα, Nr4a1 and Tacr2) and growth (Endog, Crebrtc2, Myh7, Myh1, Myh14 and Igfbp7) were identified according to GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) annotations. Our novel findings provide useful pathway information and candidate genes for future studies of genetic mechanisms underlying FCE in crucian carp. PMID:29538345

  18. Morphological, histological and molecular characterization of Myxobolus kingchowensis and Thelohanellus cf. sinensis infecting gibel carp Carassius auratus gibelio (Bloch, 1782).

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    Zhang, Bo; Zhai, Yanhua; Gu, Zemao; Liu, Yang

    2018-06-26

    A Myxobolus species and a Thelohanellus species infecting Carassius auratus gibelio (Bloch, 1782) were redescribed by their morphological, histological and molecular characterization. In the present study, the Myxobolus species infecting the muscle was identified as Myxobolus kingchowensis Chen et Ma, 1998 by the morphological and molecular data. Histologically, mature spores of M. kingchowensis were observed in the intercellular and connective tissue of muscle, though the plasmodia were not found. In addition, scattered spores also occurred in the intercellular of haematopoietic cells, intraepithelial of the renal tubules and interior of the melano-macrophage centres. Phylogenetic analysis showed that M. kingchowensis clustered in the clade of muscle-infecting Myxobolus species, further supporting muscle as the infection site of M. kingchowensis. The present Thelohanellus species infecting the gills was identified conspecific as Thelohanellus sinensis reported in Sun (2006) (mark it as T. sinensis-Sun)based on spore morphology, biological traits (host specificity and organ specificity), and molecular data. However, compared with the original description of T. sinensis Chen et Hsieh, 1960, the present Thelohanellus species and T. sinensis-Sun both infecting the gills of gibel carp are distinguishable from the original description in the host and infection site, which made the validity of T. sinensis-Sun dubious. Due to the absence of molecular data in the original description of T. sinensis, we suggest marking the present species and T. sinensis-Sun as T. cf. sinensis to avoid the confusion until T. sinensis is obtained from the type host and type infection site.

  19. Effect of azadirachtin on haematological and biochemical parameters of Argulus-infested goldfish Carassius auratus (Linn. 1758).

    Science.gov (United States)

    Kumar, Saurav; Raman, R P; Kumar, Kundan; Pandey, P K; Kumar, Neeraj; Mallesh, B; Mohanty, Snatashree; Kumar, Abhay

    2013-08-01

    Argulosis hampers aquaculture production and alters the host physiology and growth. Azadirachtin is recognized as a potential antiparasitic agent against Argulus sp. The present study aimed to investigate the effect of different concentration of azadirachtin solution on haematological and serum biochemical parameters of Argulus-infested goldfish Carassius auratus. Ninety Argulus-infested goldfish were randomly divided into six equal groups. Fish of group 1-5 were treated with azadirachtin solution through bath of 1, 5, 10, 15 and 20 mg L(-1) as T1, T2, T3, T4 and T5, respectively, and group 6 was exposed to 2% DMSO solution without azadirachtin and considered as negative control T0(-). Along with six treatment groups, a positive control T0(+) of healthy goldfish free from Argulus infestation was also maintained. Parasitic mortality was evaluated after 3 days of consecutive bath treatment. After 7 days of post-treatment, the blood and serum were drawn from each of the treatment groups and haematological and serum biochemical parameters were evaluated. Total leucocyte count (TLC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), blood glucose, total protein (TP), globulin, serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) were significantly (p azadirachtin have notable effects on activity of vital tissues function and physiology of the host. Argulus spp. from infested goldfish could be eliminated using bath treatment with solution of azadirachtin having concentration of 15 mg L(-1) and that also shifted haematological and serum biochemical parameters towards homeostasis.

  20. Report on the observed response of Javan lutungs (Trachypithecus auratus mauritius) upon encountering a reticulated python (Python reticulatus).

    Science.gov (United States)

    Tsuji, Yamato; Prayitno, Bambang; Suryobroto, Bambang

    2016-04-01

    We observed an encounter between a reticulated python (Python reticulatus) and a group of wild Javan lutungs (Trachypithecus auratus mauritius) at the Pangandaran Nature Reserve, West Java, Indonesia. A python (about 2 m in length) moved toward a group of lutungs in the trees. Upon seeing the python, an adult male and several adult female lutungs began to emit alarm calls. As the python approached, two adult and one sub-adult female jumped onto a branch near the python and began mobbing the python by shaking the branch. During the mobbing, other individuals in the group (including an adult lutung male) remained nearby but did not participate. The python then rolled into a ball-like shape and stopped moving, at which point the lutungs moved away. The total duration of the encounter was about 40 min, during which time the lutungs stopped feeding and grooming. Group cohesiveness during and after the encounter was greater than that before the encounter, indicating that lutungs adjust their daily activity in response to potential predation risk.

  1. Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus

    Directory of Open Access Journals (Sweden)

    Meixia Pang

    2018-03-01

    Full Text Available Feed efficiency is an economically crucial trait for cultured animals, however, progress has been scarcely made in the genetic analyses of feed conversion efficiency (FCE in fish because of the difficulties in measurement of trait phenotypes. In the present investigation, we present the first application of RNA sequencing (RNA-Seq combined with differentially expressed genes (DEGs analysis for identification of functional determinants related to FCE at the gene level in an aquaculture fish, crucian carp (Carassius auratus. Brain tissues of six crucian carp with extreme FCE performances were subjected to transcriptome analysis. A total of 544,612 unigenes with a mean size of 644.38 bp were obtained from Low- and High-FCE groups, and 246 DEGs that may be involved in FCE traits were identified in these two groups. qPCR confirmed that genes previously identified as up- or down-regulated by RNA-Seq were effectively up- or down-regulated under the studied conditions. Thirteen key genes, whose functions are associated with metabolism (Dgkk, Mgst3 and Guk1b, signal transduction (Vdnccsa1b, Tgfα, Nr4a1 and Tacr2 and growth (Endog, Crebrtc2, Myh7, Myh1, Myh14 and Igfbp7 were identified according to GO (Gene Ontology and KEGG (Kyoto Encyclopedia of Genes and Genomes annotations. Our novel findings provide useful pathway information and candidate genes for future studies of genetic mechanisms underlying FCE in crucian carp.

  2. Zn subcellular distribution in liver of goldfish (carassius auratus with exposure to zinc oxide nanoparticles and mechanism of hepatic detoxification.

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    Wenhong Fan

    Full Text Available Zinc Oxide Nanoparticles (ZnO NPs have attracted increasing concerns because of their widespread use and toxic potential. In this study, Zn accumulations in different tissues (gills, liver, muscle, and gut of goldfish (Carassius auratus after exposure to ZnO NPs were studied in comparison with bulk ZnO and Zn(2+. And the technique of subcellular partitioning was firstly used on the liver of goldfish to study the hepatic accumulation of ZnO NPs. The results showed that at sublethal Zn concentration (2 mg/L, bioaccumulation in goldfish was tissue-specific and dependent on the exposure materials. Compared with Zn(2+, the particles of bulk ZnO and the ZnO NPs appeared to aggregate in the environmentally contacted tissues (gills and gut, rather than transport to the internal tissues (liver and muscle. The subcellular distributions of liver differed for the three exposure treatments. After ZnO NPs exposure, Zn percentage in metal-rich granule (MRG increased significantly, and after Zn(2+ exposure, it increased significantly in the organelles. Metallothionein-like proteins (MTLP were the main target for Zn(2+, while MRG played dominant role for ZnO NPs. The different results of subcellular distributions revealed that metal detoxification mechanisms of liver for ZnO NPs, bulk ZnO, and Zn(2+ were different. Overall, subcellular partitioning provided an interesting start to better understanding of the toxicity of nano- and conventional materials.

  3. Toxicity of hydrogen sulfide to goldfish (Carassius auratus L. ) as influenced by temperature, oxygen, and bioassay techniques

    Energy Technology Data Exchange (ETDEWEB)

    Adelman, I.R.; Smith, L.L. Jr.

    1972-01-01

    Bioassays were conducted to test the effect of temperature and oxygen on H/sub 2/S toxicity to goldfish (Carassius auratus L.) and to investigate some factors that influence bioassay results. Relation of H/sub 2/S toxicity to temperature is negatively logarithmic over the range of 6.5-25 C. The mean 96-hr TL50 at 6 C was 530 ..mu..g/liter and at 25 C was 4 ..mu..g/liter. At temperatures of 14, 20, and 26 C, most acute mortality from H/sub 2/S ended by 11 days and the 11-day TL50's at these temperatures were significantly different. In bioassays with and without prior oxygen acclimation, decreasing oxygen concentrations increased H/sub 2/S toxicity. In the former, mean TL50's were 62 and 48 ..mu..g/liter H/sub 2/S at oxygen concentrations of 6 and 1.5 mg/liter, respectively, and in the latter, 71 and 53 ..mu..g/liter H/sub 2/S at the same oxygen concentrations. Variability in bioassay results was not affected by test temperatures of 14, 20, and 26 C, and in most cases 1 week of temperature acclimation was adequate. Stocks of fish responded differently after 11 days of bioassay, although differences were not detected after 4 days of bioassay. 27 references, 4 figures, 3 tables.

  4. Responses of Medullary Lateral Line Units of the Goldfish, Carassius auratus, to Amplitude-Modulated Sinusoidal Wave Stimuli

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    Ramadan Ali

    2010-01-01

    Full Text Available This paper describes the responses of brainstem lateral line units in goldfish, Carassius auratus, to constant-amplitude and to amplitude-modulated sinusoidal water motions. If stimulated with constant-amplitude sinusoidal water motions, units responded with phasic (50% or with sustained (50% increases in dicharge rate. Based on isodisplacement curves, units preferred low (33 Hz, 12.5%, mid (50 Hz, 10% and 100 Hz, 30% or high (200 Hz, 47.5% frequencies. In most units, responses were weakly phase locked to the carrier frequency. However, at a carrier frequency of 50 Hz or 100 Hz, a substantial proportion of the units exhibited strong phase locking. If stimulated with amplitude-modulated water motions, units responded with a burst of discharge to each modulation cycle, that is, units phase locked to the amplitude modulation frequency. Response properties of brainstem units were in many respects comparable to those of midbrain units, suggesting that they emerge first in the lateral line brainstem.

  5. Ectocommensal and ectoparasites in goldfish Carassius auratus (Linnaeus, 1758 in farmed in the State of São Paulo

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    Carla Renata Serantoni Moyses

    Full Text Available Concomitant infections by several parasitic genera are rare, very debilitating and often lethal to fish reared under commercial breeding conditions. Were describe a multiple and concurrent parasite infestation in cultured goldfish Carassius auratus with skin damage (nodules and/or ulceration. Fish with skin lesions underwent necropsy, and the skin and gills were scraped and examined. Histopathological examination with Hematoxylin-Eosin and Giemsa stain, and an ultrastructure study using transmission and scanning electron microscopy were conducted. In the skin, we identified multiple-parasite infestations by Gyrodactylidae, Epistylis sp., Trichodina sp., Ichthyophthirius multifiliis, Tetrahymena sp. and Ichthyobodo necator,associated with epithelial cell hyperplasia and epidermal sloughing. Although no gross lesions were observed, were identified a large number of parasites in the gills (Epistylis sp., Piscinoodinium sp., Ichthyophthirius multifiliis, Trichodina sp., Apiosoma sp., Hexamitasp. and cysts of a trematode digenean. The observed trematodes were not identified. The associated microscopic lesions were epithelial hypertrophic and hyperplasic and exhibited fusion of secondary lamellae and epithelial cell detachment. This is the first description of a protozoan Vorticella sp. parasitizing goldfish in Brazil. Multiple ectoparasitism by protozoa and Platyhelminthes, with or without apparent tissue damage, can be fatal for goldfish raised on farms with poor management.

  6. Acute toxicity of sodium chloride, pentachlorophenol, Guthion, and hexavalent chromium to fathead minnows (Pimephales promelas) and goldfish (Carassius auratus)

    Energy Technology Data Exchange (ETDEWEB)

    Adelman, I.R.; Smith, L.L. Jr.; Siesennop, G.D.

    1976-02-01

    The 96-h LC50's for sodium chloride were 7650 and 7341 mg/liter, for pentachlorophenol 0.21 and 0.22 mg/liter, for Guthion 1.9 and 2.4 mg/liter, and for hexavalent chromium 48 and 120 mg/liter, for fathead minnows (Pimephales promelas) and goldfish (Carassius auratus), respectively. Threshold LC50's were reached in 6 days for sodium chloride (7650 and 7322 mg/liter for fathead minnows and goldfish, respectively), and pentachlorophenol (0.21 and 0.21 mg/liter), but were not attained in 11 days (termination of testing) with Guthion (0.76 and 0.80 mg/liter) and hexavalent chromium (18 and 33 mg/liter). With pentachlorophenol and Guthion goldfish were initially more resistant, but by termination there was no significant difference in LC50's between the two species. With hexavalent chromium the goldfish were more resistant throughout the 11-day test, and with sodium chloride goldfish were initially more resistant but at attainment of a threshold LC50 were less resistant. Use of toxicity curves for assessment of acute mortality permits interpretation not possible in 96-h tests where LC50's are computed at 24-h intervals.

  7. EPR detection of hydroxyl radical generation and its interaction with antioxidant system in Carassius auratus exposed to pentachlorophenol

    Energy Technology Data Exchange (ETDEWEB)

    Luo Yi [Key Laboratory of Pollution Processes and Environmental Criteria (Nankai University), Ministry of Education, College of Environmental Sciences and Engineering, Nankai University, Tianjin 300071 (China); Wang Xiaorong, E-mail: yiyluo@gmail.com [State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210093 (China); Ji Liangliang; Su Yan [State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing 210093 (China)

    2009-11-15

    In the present study, direct evidence of hydroxyl radical production in livers of Carassius auratus exposed to pentachlorophenol (PCP) was provided using electron paramagnetic resonance (EPR) with spin-trapping. A dose-effect relationship was obtained between hydroxyl radical intensities and PCP exposure. It was observed that hydroxyl radical was significantly induced by 0.001 mg l{sup -1} (below the criteria for Chinese fishery water quality) of PCP exposure. A strong positive correlation (r = 0.9581, p < 0.001) was observed between PCP liver concentrations and hydroxyl radical intensities within 7 d of PCP exposure, which suggests that hydroxyl radical are mainly produced from PCP itself. However, no correlation was observed between PCP liver concentrations and hydroxyl radical intensities after 7 d, and a higher intensity of hydroxyl radical could still be observed when the PCP liver concentrations decreased to a lower level, which suggests that other mechanisms may possibly contribute to hydroxyl radical production after 7 d. The glutathione/oxidized glutathione (GSH/GSSG) ratio decreased below that of the control level during the entire period of PCP exposure (0.05 mg l{sup -1}), which suggested oxidative stress occurred.

  8. DNA methylation analysis of allotetraploid hybrids of red crucian carp (Carassius auratus red var. and common carp (Cyprinus carpio L..

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    Jun Xiao

    Full Text Available Hybridization and polyploidization may lead to divergence in adaptation and boost speciation in angiosperms and some lower animals. Epigenetic change plays a significant role in the formation and adaptation of polyploidy. Studies of the effects of methylation on genomic recombination and gene expression in allopolyploid plants have achieved good progress. However, relevant advances in polyploid animals have been relatively slower. In the present study, we used the bisexual, fertile, genetically stable allotetraploid generated by hybridization of Carassius auratus red var. and Cyprinus carpio L. to investigate cytosine methylation level using methylation-sensitive amplification polymorphism (MSAP analysis. We observed 38.31% of the methylation changes in the allotetraploid compared with the parents at 355 randomly selected CCGG sites. In terms of methylation status, these results indicate that the level of methylation modification in the allotetraploid may have increased relative to that in the parents. We also found that the major methylation changes were hypermethylation on some genomic fragments and genes related to metabolism or cell cycle regulation. These results provide circumstantial evidence that DNA methylation might be related to the gene expression and phenotype variation in allotetraploid hybrids. Our study partly fulfils the need for epigenetic research in polyploid animals, and provides evidence for the epigenetic regulation of allopolyploids.

  9. Idades e crescimento da cioba, Ocyururs chrysurus, da Costa Central do Brasil Age and growth of yellowtail snapper, Ocyururs chrysurus, from Central Coast of Brazil

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    Júlio Neves de Araújo

    2002-01-01

    Full Text Available A idade e o crescimento da cioba da costa central do Brasil foram estudados a partir de leituras de otólitos obtidos em amostragens mensais de desembarques comerciais, realizados nas cidades de Vitória e Vila Velha, entre os anos de 1998 e 1999. Através da análise do incremento marginal, foi verificada a formação anual de uma zona translúcida a partir do final do outono e durante o inverno. Foram observadas ciobas de 2 a 19 anos. Os comprimentos furcais (CF médios retrocalculados variaram de 108 mm para idade 1 a 524 mm para idade 19. A equação de Von Bertalanffy ajustada aos CF médios retrocalculados foi CFt = 567,1 (1 - e -0.130 (t + 0.773. A relação comprimento peso foi P = 2,68x10-5CF2.914, onde P = peso total em gramas. O crescimento é lento, com os incrementos anuais em peso aumentando gradativamente até atingir o máximo de 164 g entre as idades 7 e 8 anos. Os resultados deste trabalho indicam que a cioba tem longa expectativa de vida e baixas taxas de crescimento somático, características das espécies mais sensíveis à exploração pesqueira.To age and study the growth of yellowtail snapper, we analyzed readings of otoliths obtained in monthly samplings from commercial landings in the cities of Vitória and Vila Velha, from 1998 to 1999. Marginal increment analysis showed that the formation of a translucent zone occurred in the early fall and during the winter. The observed ages range from 2 to 19 years. Mean back-calculated fork lengths (FL range from 108 mm for age 1 to 524 mm for age 19. The Von Bertalanffy growth equation fitted to the mean back-calculated FL was FLt = 567,1 (1 - e -0.130 (t + 0.773, The length-weight relationship was W= 2,68x10-5FL2.914, where W = whole weight in grams. Yellowtail snapper has a slow growth with annual growth increments in weight raising progressively to the maximum of 164 g between the 7 and 8 years. The yellowtail snapper has long lifespan and slow growth rates, features of

  10. Initial Response by a Native Beetle, Chrysochus auratus (Coleoptera: Chrysomelidae), to a Novel Introduced Host-Plant, Vincetoxicum rossicum (Gentianales: Apocynaceae).

    Science.gov (United States)

    deJonge, R B; Bourchier, R S; Smith, S M

    2017-06-01

    Native insects can form novel associations with introduced invasive plants and use them as a food source. The recent introduction into eastern North America of a nonnative European vine, Vincetoxicum rossicum (Kleopow) Barbar., allows us to examine the initial response of a native chrysomelid beetle, Chrysochus auratus F., that feeds on native plants in the same family as V. rossicum (Apocynaceae). We tested C. auratus on V. rossicum and closely related or co-occurring native plants (Apocynum spp., Asclepias spp., and Solidago canadensis L.) using all life stages of the beetle in lab, garden, and field experiments. Experiments measured feeding (presence or absence and amount), survival, oviposition, and whether previous exposure to V. rossicum in the lab or field affected adult beetle feeding. Beetles fed significantly less on V. rossicum than on native Apocynum hosts. Adult beetles engaged in exploratory feeding on leaves of V. rossicum and survived up to 10 d. Females oviposited on V. rossicum, eggs hatched, and larvae fed initially on the roots; however, no larvae survived beyond second instar. Beetles collected from Apocynum cannabinum L. field sites intermixed with V. rossicum were less likely to feed on this novel nonnative host than those collected from colonies further from and less likely to be exposed to V. rossicum (>5 km). Our experimental work indicates that V. rossicum may act as an oviposition sink for C. auratus and that this native beetle has not adapted to survive on this recently introduced novel host plant. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  11. A new species of Dentiphilometra (Nematoda: Philometridae) from the musculature of the gray snapper Lutjanus griseus (osteichthyes) off the Caribbean coast of Mexico.

    Science.gov (United States)

    González-Solís, David; Moravec, Frantisek; Paredes, Vielka M Tuz

    2007-10-01

    A new nematode, Dentiphilometra lutjani n. sp. (Philometridae), is described from gravid females (the male is unknown) collected from the body musculature of the marine perciform fish gray snapper, Lutjanus griseus (Lutjanidae), from the Bay of Chetumal and southern coast of Quintana Roo, off the Caribbean coast of Mexico. The new species differs from the only other congener, Dentiphilometra monopteri, from the swamp eel Monopterus albus in China, mainly in the body length of gravid female (15.40-53.21 mm), the shape of the posterior body end (not markedly narrowed, with low caudal projections), the esophageal gland (maximum width near its posterior end), and the length (344-483 microm) of larvae from the uterus; both species also differ in their host types (marine perciform fish vs. freshwater swamp eel) and geographical distribution (Mexico vs. China).

  12. A new species of Trichosomoididae (Nematoda) from skin of red snapper, Lutjanus campechanus (Perciformes: Lutjanidae), on the Texas-Louisiana shelf, northern Gulf of Mexico.

    Science.gov (United States)

    Ruiz, Carlos F; Ray, Candis L; Cook, Melissa; Grace, Mark A; Bullard, Stephen A

    2013-04-01

    Eggs and larvae of Huffmanela oleumimica n. sp. infect red snapper, Lutjanus campechanus (Poey, 1860), were collected from the Texas-Louisiana Shelf (28°16'36.58″N, 93°03'51.08″W) and are herein described using light and scanning electron microscopy. Eggs in skin comprised fields (1-5 × 1-12 mm; 250 eggs/mm(2)) of variously oriented eggs deposited in dense patches or in scribble-like tracks. Eggs had clear (larvae indistinct, principally vitelline material), amber (developing larvae present) or brown (fully developed larvae present; little, or no, vitelline material) shells and measured 46-54 μm (x = 50; SD ± 1.6; n = 213) long, 23-33 (27 ± 1.4; 213) wide, 2-3 (3 ± 0.5; 213) in eggshell thickness, 18-25 (21 ± 1.1; 213) in vitelline mass width, and 36-42 (39 ± 1.1; 213) in vitelline mass length with protruding polar plugs 5-9 (7 ± 0.6; 213) long and 5-8 (6 ± 0.5; 213) wide. Fully developed larvae were 160-201 (176 ± 7.9) long and 7-8 (7 ± 0.5) wide, had transverse cuticular ridges, and were emerging from some eggs within and beneath epidermis. The new species differs from its congeners by having eggs 110 μm long with transverse cuticular ridges. The eggs lack a spindle-shaped envelope, polar filaments, and eggshell ridges. This is the first report of a species of Huffmanela from a snapper (Lutjanidae) or from the Gulf of Mexico. A table of egg and larval characteristics, hosts, and localities for Huffmanela spp. is provided.

  13. Growth performance of pearl goldfish juvenile carassius auratus cultured in 3 ppt salinity with different exposure time of electric field

    Directory of Open Access Journals (Sweden)

    Kukuh Nirmala

    2015-04-01

    Full Text Available ABSTRACTGrowth rate of pearl goldfish juvenile Carassius auratus relatively slow to reach market size which will take approximately three months. To accelerate its growth can be done by providing exposure of the fish to low-power electric fields (10 V via 3 ppt salinity water, with the goal of providing the close isoosmotic conditions, and also to streamline the flow of electricity from the electrodes to the body of the fish. This study aims to calculate the survival and growth rate of pearl goldfish juvenile of S sizes (2‒4 cm of body length which were maintained at 3 ppt salinity water and treated by different exposure time of electric field (zero, two, four, and six minutes before feeding with 10 volt electric power. Fish were cultured at a density of 2 fish/L in the (20×30×20 cm3 aquaria in volume of 6 L of water. Test fish had an average body length of 4.11±0.05 cm and the average body weight of 2.89±0.05 g. Exposure time of electric field were zero, two, four, and six minutes before the fish are fed, performed every day as much as three times i.e. morning, afternoon, and evening. The research design used was completely randomized design with four treatments, namely 0, 2, 4, and 6 (time for exposure is zero/control, two, four, and six minute with three replications. The results show test fishes exposed to 10 volt electrical field for zero, two, four, and six minutes, have no significant effect on survival rate (P>0.05. For growth performance, four minute exposure treatment gives the best results compared to controls (P<0.05, supported by an increase in the percentage of the ratio of gut length to body length of the fish and higher feed efficiency.Keywords: long exposure to the electric field, growth performance, pearl goldfishABSTRAKPertumbuhan benih ikan hias maskoki mutiara Carassius auratus relatif lambat, karena untuk mencapai ukuran jual memerlukan waktu sekitar tiga bulan. Untuk mempercepat pertumbuhannya dapat dilakukan dengan

  14. Radioactive tracers in the study of energy turnover by a grazing insect (Chrysochus auratus Fab. ; coleoptera chrysomelidae)

    Energy Technology Data Exchange (ETDEWEB)

    Williams, Jr, E C; Reichle, D E

    1968-01-01

    Radioisotope tags of rubidium-86 and phosphorus-32 were used to measure food consumption by the chrysomelid beetle Chrysochus auratus feeding upon the old field herb Apocynum cannabinum. The elimination of isotope by beetles which had fed upon tagged plants was used to estimate the rates of food consumption. For individuals at isotopic equilibrium with their food base, isotope input (I) equals the biological turnover of isotope: I = kQ/sub e/M/a, where k is the turnover rate of isotope, Q/sub e/ is the equilibrium concentration of isotope in the beetles, M is mass (mg dry wt), and a is the assimilation coefficient for the particular radioisotope. Turnover rates (k) were calculated from biological half-lives (T/sub b/), where k = 0.693/T/sub b/. The biological half-life of YWRb in Chrysochus was one day, while that of TSP was 7.5 days in females and 10.2 days in males. The calorific value of Apocynum leaves was 4625 cal/g dry wt (5640 cal/g ash-free wt); that of Chrysochus was 5227 cal/g dry wt (5537 cal/g ash-free wt). Individuals consumed 56.8% (15.9 mg) of their dry body weight in food per day. Of the ingested foliage, 43% of the ash-free dry matter was digested, 56% of the energy assimilated, and 71% of the ash content assimilated. The calorific value (cal/g) of digested food was 1.17 times greater than that consumed. Beetles assimilated 87% of the YWRb and 74% of the TSP present in food. Ranking of turnover times showed Chrysochus to be most conservative in its expenditure of food energy and dry matter, followed by mineral constituents. 9 references, 3 figures, 3 tables.

  15. Adolescent anabolic/androgenic steroids: Aggression and anxiety during exposure predict behavioral responding during withdrawal in Syrian hamsters (Mesocricetus auratus).

    Science.gov (United States)

    Ricci, Lesley A; Morrison, Thomas R; Melloni, Richard H

    2013-11-01

    In the U.S. and worldwide anabolic/androgenic steroid use remains high in the adolescent population. This is concerning given that anabolic/androgenic steroid use is associated with a higher incidence of aggressive behavior during exposure and anxiety during withdrawal. This study uses pubertal Syrian hamsters (Mesocricetus auratus) to investigate the hypothesis that an inverse behavioral relationship exists between anabolic/androgenic steroid-induced aggression and anxiety across adolescent exposure and withdrawal. In the first experiment, we examined aggression and anxiety during adolescent anabolic/androgenic steroid exposure and withdrawal. Adolescent anabolic/androgenic steroid administration produced significant increases in aggression and decreases in anxiety during the exposure period followed by significant decreases in aggression and increases in anxiety during anabolic/androgenic steroid withdrawal. In a second experiment, anabolic/androgenic steroid exposed animals were separated into groups based on their aggressive response during the exposure period and then tested for anxiety during exposure and then for both aggression and anxiety during withdrawal. Data were analyzed using a within-subjects repeated measures predictive analysis. Linear regression analysis revealed that the difference in aggressive responding between the anabolic/androgenic steroid exposure and withdrawal periods was a significant predictor of differences in anxiety for both days of testing. Moreover, the combined data suggest that the decrease in aggressive behavior from exposure to withdrawal predicts an increase in anxiety-like responding within these same animals during this time span. Together these findings indicate that early anabolic/androgenic steroid exposure has potent aggression- and anxiety-eliciting effects and that these behavioral changes occur alongside a predictive relationship that exists between these two behaviors over time. © 2013.

  16. NMR-based metabolomics approach to study the toxicity of lambda-cyhalothrin to goldfish (Carassius auratus)

    International Nuclear Information System (INIS)

    Li, Minghui; Wang, Junsong; Lu, Zhaoguang; Wei, Dandan; Yang, Minghua; Kong, Lingyi

    2014-01-01

    Highlights: •A goldfish model was established to investigate the toxicity of lambda-cyhalothrin (LCT) exposure on multiple organs. •NMR based metabolomics approach were firstly used to provide a global view of the toxicity of LCT. •LCT induced neurotransmitters and osmoregulatory imbalances, oxidative stress, energy and amino acid metabolic disorders. •Glutamate–glutamine–GABA axis as a potential target for LCT toxicity was first found. -- Abstract: In this study, a 1 H nuclear magnetic resonance (NMR) based metabolomics approach was applied to investigate the toxicity of lambda-cyhalothrin (LCT) in goldfish (Carassius auratus). LCT showed tissue-specific damage to gill, heart, liver and kidney tissues of goldfish. NMR profiling combined with statistical methods such as orthogonal partial least squares discriminant analysis (OPLS-DA) and two-dimensional statistical total correlation spectroscopy (2D-STOCSY) was developed to discern metabolite changes occurring after one week LCT exposure in brain, heart and kidney tissues of goldfish. LCT exposure influenced levels of many metabolites (e.g., leucine, isoleucine and valine in brain and kidney; lactate in brain, heart and kidney; alanine in brain and kidney; choline in brain, heart and kidney; taurine in brain, heart and kidney; N-acetylaspartate in brain; myo-inositol in brain; phosphocreatine in brain and heart; 2-oxoglutarate in brain; cis-aconitate in brain, and etc.), and broke the balance of neurotransmitters and osmoregulators, evoked oxidative stress, disturbed metabolisms of energy and amino acids. The implication of glutamate–glutamine–gamma-aminobutyric axis in LCT induced toxicity was demonstrated for the first time. Our findings demonstrated the applicability and potential of metabolomics approach for the elucidation of toxicological effects of pesticides and the underlying mechanisms, and the discovery of biomarkers for pesticide pollution in aquatic environment

  17. Brain Mapping of Ghrelin O-Acyltransferase in Goldfish (Carassius Auratus): Novel Roles for the Ghrelinergic System in Fish?

    Science.gov (United States)

    Blanco, Ayelén M; Sánchez-Bretaño, Aída; Delgado, María J; Valenciano, Ana I

    2016-06-01

    Ghrelin O-acyltransferase (GOAT) is the enzyme responsible for acylation of ghrelin, a gut-brain hormone with important roles in many physiological functions in vertebrates. Many aspects of GOAT remain to be elucidated, especially in fish, and particularly its anatomical distribution within the different brain areas has never been reported to date. The present study aimed to characterize the brain mapping of GOAT using RT-qPCR and immunohistochemistry in a teleost, the goldfish (Carassius auratus). Results show that goat transcripts are expressed in different brain areas of the goldfish, with the highest levels in the vagal lobe. Using immunohistochemistry, we also report the presence of GOAT immunoreactive cells in different encephalic areas, including the telencephalon, some hypothalamic nuclei, pineal gland, optic tectum and cerebellum, although they are especially abundant in the hindbrain. Particularly, an important signal is observed in the vagal lobe and some fiber tracts of the brainstem, such as the medial longitudinal fasciculus, Mauthneri fasciculus, secondary gustatory tract and spinothalamic tract. Most of the forebrain areas where GOAT is detected, particularly the hypothalamic nuclei, also express the ghs-r1a ghrelin receptor and other appetite-regulating hormones (e.g., orexin and NPY), supporting the role of ghrelin as a modulator of food intake and energy balance in fish. Present results are the first report on the presence of GOAT in the brain using imaging techniques. The high presence of GOAT in the hindbrain is a novelty, and point to possible new functions for the ghrelinergic system in fish. Anat Rec, 299:748-758, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  18. NMR-based metabolomics approach to study the toxicity of lambda-cyhalothrin to goldfish (Carassius auratus)

    Energy Technology Data Exchange (ETDEWEB)

    Li, Minghui [State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009 (China); Wang, Junsong, E-mail: wang.junsong@gmail.com [Center for Molecular Metabolism, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, 200 Xiao Ling Wei Street, Nanjing 210094 (China); Lu, Zhaoguang; Wei, Dandan; Yang, Minghua [State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009 (China); Kong, Lingyi, E-mail: cpu_lykong@126.com [State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009 (China)

    2014-01-15

    Highlights: •A goldfish model was established to investigate the toxicity of lambda-cyhalothrin (LCT) exposure on multiple organs. •NMR based metabolomics approach were firstly used to provide a global view of the toxicity of LCT. •LCT induced neurotransmitters and osmoregulatory imbalances, oxidative stress, energy and amino acid metabolic disorders. •Glutamate–glutamine–GABA axis as a potential target for LCT toxicity was first found. -- Abstract: In this study, a {sup 1}H nuclear magnetic resonance (NMR) based metabolomics approach was applied to investigate the toxicity of lambda-cyhalothrin (LCT) in goldfish (Carassius auratus). LCT showed tissue-specific damage to gill, heart, liver and kidney tissues of goldfish. NMR profiling combined with statistical methods such as orthogonal partial least squares discriminant analysis (OPLS-DA) and two-dimensional statistical total correlation spectroscopy (2D-STOCSY) was developed to discern metabolite changes occurring after one week LCT exposure in brain, heart and kidney tissues of goldfish. LCT exposure influenced levels of many metabolites (e.g., leucine, isoleucine and valine in brain and kidney; lactate in brain, heart and kidney; alanine in brain and kidney; choline in brain, heart and kidney; taurine in brain, heart and kidney; N-acetylaspartate in brain; myo-inositol in brain; phosphocreatine in brain and heart; 2-oxoglutarate in brain; cis-aconitate in brain, and etc.), and broke the balance of neurotransmitters and osmoregulators, evoked oxidative stress, disturbed metabolisms of energy and amino acids. The implication of glutamate–glutamine–gamma-aminobutyric axis in LCT induced toxicity was demonstrated for the first time. Our findings demonstrated the applicability and potential of metabolomics approach for the elucidation of toxicological effects of pesticides and the underlying mechanisms, and the discovery of biomarkers for pesticide pollution in aquatic environment.

  19. Identification of individual adult female Javan lutungs (Trachypithecus auratus sondaicus) by using patterns of dark pigmentation in the pubic area.

    Science.gov (United States)

    Tsuji, Yamato; Widayati, Kanthi Arum; Hadi, Islamul; Suryobroto, Bambang; Watanabe, Kunio

    2013-01-01

    In a series of field surveys of wild Javan lutungs (Trachypithecus auratus sondaicus) conducted at Pangandaran Nature Reserve in West Java, Indonesia, from 2011 to 2012, we tried to use a method of individual identification by using individual-specific patterns of dark pigmentation in the pubic area. During the 2011 dry season, we used a digital SLR camera with a 400-mm telephoto lens to photograph the pubic area of each individual of a habituated group. These photographs were the basis for identifying 14 different adult females. During the rainy season of 2011 and the dry season of 2012, we checked the presence/absence of each of the identified individuals and found that these patterns were stable, at least during our study period. We found that two adult females and one adult female disappeared from the subject group between the first and second and between the second and third surveys, respectively, and that one adult female gave birth between the first and second surveys, but the infant had disappeared from the group between the second and third surveys. We could not confirm the validity of the method for juvenile females because of the dense white hair in their pubic areas and the fact that few individuals had clear patterns. Furthermore, we could not use this method for males because of the lack of pigmentation in the pubic area. As patterns of pigmentation in the pubic area are known to be present in other Trachypithecus species, our method can be useful for identification of individual adult females of these species, on which few individual-based behavioral studies have been conducted. Collecting individual-based behavioral data would enable us to track the presence of individuals in groups or movements between groups; determine the effects of social rank and age on within-group competition and copulation; and examine population data.

  20. The origin and evolution of the surfactant system in fish: insights into the evolution of lungs and swim bladders.

    Science.gov (United States)

    Daniels, Christopher B; Orgeig, Sandra; Sullivan, Lucy C; Ling, Nicholas; Bennett, Michael B; Schürch, Samuel; Val, Adalberto Luis; Brauner, Colin J

    2004-01-01

    Several times throughout their radiation fish have evolved either lungs or swim bladders as gas-holding structures. Lungs and swim bladders have different ontogenetic origins and can be used either for buoyancy or as an accessory respiratory organ. Therefore, the presence of air-filled bladders or lungs in different groups of fishes is an example of convergent evolution. We propose that air breathing could not occur without the presence of a surfactant system and suggest that this system may have originated in epithelial cells lining the pharynx. Here we present new data on the surfactant system in swim bladders of three teleost fish (the air-breathing pirarucu Arapaima gigas and tarpon Megalops cyprinoides and the non-air-breathing New Zealand snapper Pagrus auratus). We determined the presence of surfactant using biochemical, biophysical, and morphological analyses and determined homology using immunohistochemical analysis of the surfactant proteins (SPs). We relate the presence and structure of the surfactant system to those previously described in the swim bladders of another teleost, the goldfish, and those of the air-breathing organs of the other members of the Osteichthyes, the more primitive air-breathing Actinopterygii and the Sarcopterygii. Snapper and tarpon swim bladders are lined with squamous and cuboidal epithelial cells, respectively, containing membrane-bound lamellar bodies. Phosphatidylcholine dominates the phospholipid (PL) profile of lavage material from all fish analyzed to date. The presence of the characteristic surfactant lipids in pirarucu and tarpon, lamellar bodies in tarpon and snapper, SP-B in tarpon and pirarucu lavage, and SPs (A, B, and D) in swim bladder tissue of the tarpon provide strong evidence that the surfactant system of teleosts is homologous with that of other fish and of tetrapods. This study is the first demonstration of the presence of SP-D in the air-breathing organs of nonmammalian species and SP-B in actinopterygian

  1. Peroral Echinococcus multilocularis egg inoculation in Myodes glareolus, Mesocricetus auratus and Mus musculus (CD-1 IGS and C57BL/6j)

    DEFF Research Database (Denmark)

    Woolsey, Ian David; Jensen, Per Moestrup; Deplazes, Peter

    2016-01-01

    Echinococcus multilocularis transmission predominantly occurs in Europe between the red fox (Vulpes vulpes) and various species of rodent intermediate hosts. We infected 3 species of rodent, Myodes glareolus (n = 47), Mesocricetus auratus (n = 11) and outbred Mus musculus (CD-1 IGS) (n = 9...

  2. Genetic evidence for gonochoristic reproduction in gynogenetic silver crucian carp (Carassius auratus gibelio bloch) as revealed by RAPD assays.

    Science.gov (United States)

    Zhou, L; Wang, Y; Gui, J F

    2000-11-01

    Sex evolution has been a debating focus in evolutionary genetics. In lower vertebrates of reptiles, amphibians, and fish, a species or a bioform reproduces either sexually or asexually but never both. A few species were found to consist of all females in fish. These all-female species can propagate by asexual reproduction modes, such as gynogenesis and hybridogenesis. However, the coexistence of sexuality and asexuality in a single species was recently noted only in a cyprinid fish silver crucian carp, Carassius auratus gibelio. This fish had been demonstrated to be capable of gynogenesis stimulated by sperm from other related species. Surprisingly, natural populations of this fish consist of a minor but significant portion (approx. 20%) of males. As different clones with specific phenotypic and genetic characteristics have been found, and RAPD markers specific to each clone have recently been identified, this fish offers many advantages for analyzing whether or not genetic recombination occurs between different clones. In this study, artificial propagation was performed in clone F and clone D. Ovulated eggs from clone F were divided into two parts and respectively inseminated with sperm from a clone D male and from a red common carp (Cyprinus carpio) male. The control clone D individuals were selected from gynogenetic offspring of clone D activated by sperm of red common carp. The phenotype and sex ratio in the experimental groups were also observed. Using RAPD molecular markers, which allow for reliable discrimination and genetic analysis of different clones, we have revealed direct molecular evidence for gonochoristic reproduction in the gynogenetic silver crucian carp and confirmed a previous hypothesis that the silver crucian carp might reproduce both gynogenetically and gonochoristically. Therefore, we conclude that the silver crucian carp possesses two reproductive modes, i.e., gynogenetic and gonochoristic reproduction. The response mechanism of two

  3. Feeding ecology of juvenile dog snapper Lutjanus jocu (Bloch and Shneider, 1801 (Lutjanidae in intertidal mangrove creeks in Curuçá estuary (Northern Brazil

    Directory of Open Access Journals (Sweden)

    Dijane Pantoja Monteiro

    2009-12-01

    Full Text Available The diet and feeding ecology of juvenile dog snapper (Lutjanus jocu were investigated in 92 specimens collected in four intertidal mangrove creeks of Curuçá estuary, Northern Brazil, between September 2003 and July 2004. No significant differences in total length were found between the sampling months. Feeding intensity was high as indicated by high stomach fullness index and low vacuity index. The most important prey was Penaeidae, followed by Grapsidae and Porcellanidae. The diet of juvenile dog snapper showed clear seasonal differences. Specimens from dry season (September and November and dry / wet season transition (January, were specialists feeding exclusively on Penaeidae. However, the specimens from wet season (March and May and wet /dry season transition (July that consumed mainly Grapsidae, Penaeidae and Porcellanidae were generalist. These seasonal changes in diet could be related to variations in food availability.A dieta e a ecologia alimentar de juvenis de Lutjanus jocu foram verificadas em 92 espécimes coletados em quatro canais de maré do estuário do rio Curuçá, Norte do Brasil, entre setembro de 2003 e julho de 2004. O comprimento total dos peixes coletados não apresentou diferenças significativas entre os meses amostrados. A intensidade alimentar foi elevada conforme indicado pelos altos valores do índice de repleção estomacal e os baixos valores do índice de vacuidade. A presa mais importante foi Penaeidae, seguida por Grapsidae e Porcellanidae. A dieta de juvenis de L. jocu apresentou diferenças sazonais evidentes. Os espécimes da estação seca (setembro e novembro e transição seca/chuvosa (janeiro foram considerados especialistas alimentando-se exclusivamente de Penaeidae. No entanto, os espécimes da estação chuvosa (março e maio e da transição chuvosa/seca (julho, que alimentaram-se principalmente de Grapsidae, Penaeidae e Porcellanidae, foram considerados generalistas. Esta mudança sazonal na dieta

  4. Effects of replacing fishmeal with animal by-products meal supplementation in diets on the growth and nutrient utilization of mangrove red snapper

    Science.gov (United States)

    Jamil, Khalid; Abbas, Ghulam; Akhtar, Rukhsana; Lin, Hong; Li, Zhenxing

    2007-07-01

    A feeding trial was conducted for 75 d to evaluate the nutritive value of a mixture of animal by-products (MAB) as a possible protein source in diets for juvenile mangrove red snapper, Lutjanus argentimaculatus (mean initial body weight, 30 g). Fish were fed one of five isonitrogenous diets (40% crude protein) replacing 0, 25% (MAB25), 50% (MAB50), 75% (MAB75) and 100% (MAB100) of fish meal protein with similar percentages of MAB. The MAB consisted of 25% cow liver meal, 20% leather meal, 20% meat and bone meal, 15% blood meal, 10% APC (poultry feather meal), 8% poultry manure dried, 1.5% choline and 0.5% chromic oxide. After 75 d of feeding, fish fed with diets MAB50, MAB75 and MAB100 exhibited significantly lower growth performance than that of fish fed with control and MAB25 diets. The optimum level of MAB was estimated to be 23%. Replacement of fish meal by MAB23% showed the following performance: maximum weight gain, 510%; SGR, 2.39% and FCE, 2.83%. The MAB substitution up to 75% of fish meal protein in diets did not show differences in apparent protein digestibility (83.6% for MAB25, 79.2% for MAB50, 78.7% for MAB75) compared with control (83.4%), whereas in MAB100 group digestibility (65.3%) was significantly lower than in other groups. The apparent phosphorus absorption of test diet groups was significantly higher (37.1% for MAB25, 28.5% for MAB50, 55.6% for MAB75 and 54.5% for MAB100) than that of control (11.2%). The levels of protein and ash in the whole body, carcass and viscera increased as MAB substitution in diets increased, whereas lipids and moisture remained consistent among all treatment groups. These results showed that approximately 23% of fish meal protein could be replaced by a mixture of animal by-products for juvenile snapper growing from 30 g to 167 g in 75 d without compromising growth performance and feed efficiency.

  5. Laboratory evaluation of different formulations of Stress Coat® for slime production in goldfish (Carassius auratus) and koi (Cyprinus carpio).

    Science.gov (United States)

    Shivappa, Raghunath B; Christian, Larry S; Law, Jerry M; Lewbart, Gregory A

    2017-01-01

    A study was carried out to assess the effect of Stress Coat ® on slime production in goldfish ( Carassius auratus) and koi ( Cyprinus carpio ). The study also investigated histological changes that might be associated with slime producing cells, and wound healing in koi. Several formulations of Stress Coat ® were investigated and the results showed that polyvinylpyrrolidone (PVP), also known as povidone, an ingredient of Stress Coat ® , when used alone, showed significantly higher slime production in goldfish than salt and Stress Coat ® without PVP after 25 h. The results also showed that koi treated with compounds containing PVP showed better wound healing than those not exposed to PVP. Histology results showed no difference between compounds tested with regards to density and number of slime producing cells.

  6. Influence of ammonia on forming the toxicity of waters from the surface sources of water supply determined on Carassius auratus gibelio (Bloch, 1782

    Directory of Open Access Journals (Sweden)

    Е. Arystarkhova

    2018-03-01

    Full Text Available Purpose. Determination of the influence of ammonia in waters from surface sources of water supply of Zhytomyr city on forming the toxicity of these waters determined by test-reactions of atypical motor activity of Carassius auratus gibelio (Bloch, 1782 with the use of the «time sampling» method during 2012–2014. Methodology. Biotesting was performed at the Municipal Enterprise "Zhytomyrvodokanal". Water samples were taken once a month time from the Teteriv river reservoirs and tap water network and then placed into aquaria (8 dm3 on a group. Control and experimental groups of fish were formed according to the following scheme: control group — samples of settled (24 hours tap water; experimental group D-1 — water samples from the Denyshivske reservoir; experimental group D-2 — water samples from the Vidsichne water intake. Test specimens were females of C. auratus gibelio. Biotesting was conducted using the «time sampling» method by keeping fish (n=30 in water for 12 hours. The toxicity indexes of waters were calculated on the basis of the following test-reactions: spiral-like and vector movements, jumping out from water, immobilization and death of fish. Statistical processing of study results were performed using cross-correlation and regression analysis in MS Excel 2007 and Statistica-6. Findings. The study showed an effect of ammonia on the toxicity of waters from reservoirs of the Teteriv river that was determined by atypical motor activity with the use of the «time sampling» method, which consisted in the instantaneous fixation of the number of individuals that favored one or another act of behavior. It was shown that females not adapted to the action of ammonia reacted to its concentration in water of more than 0.55 mg/dm3 by disorders in movements. Unlike fish of experience groups, only single pathological acts were observed in the control group. A positive moderate relationship, which had a tendency to an increase, was

  7. Laboratory evaluation of different formulations of Stress Coat® for slime production in goldfish (Carassius auratus and koi (Cyprinus carpio

    Directory of Open Access Journals (Sweden)

    Raghunath B. Shivappa

    2017-09-01

    Full Text Available A study was carried out to assess the effect of Stress Coat® on slime production in goldfish (Carassius auratus and koi (Cyprinus carpio. The study also investigated histological changes that might be associated with slime producing cells, and wound healing in koi. Several formulations of Stress Coat® were investigated and the results showed that polyvinylpyrrolidone (PVP, also known as povidone, an ingredient of Stress Coat®, when used alone, showed significantly higher slime production in goldfish than salt and Stress Coat® without PVP after 25 h. The results also showed that koi treated with compounds containing PVP showed better wound healing than those not exposed to PVP. Histology results showed no difference between compounds tested with regards to density and number of slime producing cells.

  8. N-methyl-D-aspartate receptor antagonist MK-801 impairs learning but not memory fixation or expression of classical fear conditioning in goldfish (Carassius auratus).

    Science.gov (United States)

    Xu, X; Davis, R E

    1992-04-01

    The amnestic effects of the noncompetitive antagonist MK-801 on visually mediated, classic fear conditioning in goldfish (Carassius auratus) was examined in 5 experiments. MK-801 was administered 30 min before the training session on Day 1 to look for anterograde amnestic effects, immediately after training to look for retrograde amnestic effects, and before the training or test session, or both, to look for state-dependence effects. The results showed that MK-801 produced anterograde amnesia at doses that did not produce retrograde amnesia or state dependency and did not impair the expression of conditioned or unconditioned branchial suppression responses (BSRs) to the conditioned stimulus. The results indicate that MK-801 disrupts the mechanism of learning of the conditioned stimulus-unconditioned stimulus relation. Evidence is also presented that the learning processes that are disrupted by MK-801 occur during the initial stage of BSR conditioning.

  9. Perception of scent over-marks by golden hamsters (Mesocricetus auratus): novel mechanisms for determining which individual's mark is on top.

    Science.gov (United States)

    Johnston, R E; Bhorade, A

    1998-09-01

    Hamsters preferentially remember or value the top scent of a scent over-mark. What cues do they use to do this? Using habituation-discrimination techniques, we exposed male golden hamsters (Mesocricetus auratus) on 3 to 4 trials to genital over-marks from 2 females and then tested subjects for their familiarity with these 2 scents compared with that of a novel female's secretion. Preferential memory for 1 of the 2 individuals' scents did not occur if the 2 marks did not overlap or did not overlap but differed in age, but it did occur if a region of overlap existed or 1 mark apparently occluded another (but did not overlap it). Thus, hamsters use regions of overlap and the spatial configuration of scents to evaluate over-marks. These phenomena constitute evidence for previously unsuspected perceptual abilities, including olfactory scene analysis, which is analogous to visual and auditory scene analysis.

  10. Variation in fatty acid composition of the bigeye snapper Lutjanus lutjanus collected in coral reef habitats of the Malaysian South China Sea.

    Science.gov (United States)

    Arai, Takaomi; Amalina, Razikin; Bachok, Zainudin

    2015-12-01

    In order to understand trophic ecology, habitat use and migration of coral reef fish, fatty acid composition and levels were examined in the bigeye snapper Lutjanus lutjanus collected in the Malaysian South China Sea. Proportions of saturated fatty acids (SAFA) ranged from 55.0% to 66.5%, with the highest proportions in fatty acids, the second highest was monounsaturated fatty acids (MUFA) ranged from 30.7% to 40.2% while the proportion of polyunsaturated fatty acids (PUFA) was the lowest ranged from 2.8% to 4.8%. Palmitic acid (16:0) was the most common in SAFA, oleic acid (C18:1ω9c) was the dominant in MUFA and linolenic acid (C18:3n3) showed the highest value in PUFA. Fatty acid concentrations, especially in SAFA and MUFA, increased with fish growth, suggesting diet and habitat shifts during the fish life history. Most of the fish had more than 1 of EPA: DHA ratio, which suggested that diets of L. lutjanus tended to be higher trophic organisms such as zooplankton and crustacean in coral ecosystem. The diet shift revealed by the composition and levels of the fatty acid profile revealed potential pattern in the habitat use and migration scale in coral reef environment of L. lutjanus.

  11. Metal accumulation and antioxidant defenses in the freshwater fish Carassius auratus in response to single and combined exposure to cadmium and hydroxylated multi-walled carbon nanotubes

    International Nuclear Information System (INIS)

    Qu, Ruijuan; Wang, Xinghao; Wang, Zunyao; Wei, Zhongbo; Wang, Liansheng

    2014-01-01

    Highlights: • Cd and OH-MWCNTs have a synergistic effect on Carassius auratus. • OH-MWCNTs significantly increased Cd accumulation in liver after 12 d exposure. • Co-exposure to Cd and OH-MWCNTs evoked severe hepatic oxidative stress. - Abstract: The effects of cadmium, hydroxylated multi-walled carbon nanotubes, and their mixture on metal accumulation and antioxidant defenses were studied using the goldfish Carassius auratus as the test organism. The fish were exposed to 0.1 mg/L Cd, 0.5 mg/L OH-MWCNTs, or 0.1 mg/L Cd + 0.5 mg/L OH-MWCNTs for 3 and 12 days. Then, the Cd concentration was determined in the gill, liver and muscle. Moreover, hepatic antioxidant enzyme activity (superoxide dismutase, catalase and glutathione peroxidase), glutathione level and malondialdehyde content were also measured. A continuous accumulation of Cd was observed throughout the experimental period. Cd accumulation in tissues occurred in the following order: gill > liver > muscle at 3 days and liver > gill > muscle at 12 days. The concentrations of Cd in the livers of fish exposed to the combination of Cd + OH-MWCNTs were significantly higher than those in fish exposed to either single chemical after 12 d of exposure. Meanwhile, the mixture evoked severe oxidative stress in the exposed fish, as indicated by significant inhibition of SOD, CAT and GPx activity, a remarkable decrease in GSH level, and simultaneous elevation of MDA content. These results suggested that the effect of the combined factors on metal accumulation and oxidative stress biomarkers was more obvious than that of single factors at longer exposure durations

  12. Metal accumulation and antioxidant defenses in the freshwater fish Carassius auratus in response to single and combined exposure to cadmium and hydroxylated multi-walled carbon nanotubes

    Energy Technology Data Exchange (ETDEWEB)

    Qu, Ruijuan; Wang, Xinghao; Wang, Zunyao, E-mail: wangzun315cn@163.com; Wei, Zhongbo; Wang, Liansheng

    2014-06-30

    Highlights: • Cd and OH-MWCNTs have a synergistic effect on Carassius auratus. • OH-MWCNTs significantly increased Cd accumulation in liver after 12 d exposure. • Co-exposure to Cd and OH-MWCNTs evoked severe hepatic oxidative stress. - Abstract: The effects of cadmium, hydroxylated multi-walled carbon nanotubes, and their mixture on metal accumulation and antioxidant defenses were studied using the goldfish Carassius auratus as the test organism. The fish were exposed to 0.1 mg/L Cd, 0.5 mg/L OH-MWCNTs, or 0.1 mg/L Cd + 0.5 mg/L OH-MWCNTs for 3 and 12 days. Then, the Cd concentration was determined in the gill, liver and muscle. Moreover, hepatic antioxidant enzyme activity (superoxide dismutase, catalase and glutathione peroxidase), glutathione level and malondialdehyde content were also measured. A continuous accumulation of Cd was observed throughout the experimental period. Cd accumulation in tissues occurred in the following order: gill > liver > muscle at 3 days and liver > gill > muscle at 12 days. The concentrations of Cd in the livers of fish exposed to the combination of Cd + OH-MWCNTs were significantly higher than those in fish exposed to either single chemical after 12 d of exposure. Meanwhile, the mixture evoked severe oxidative stress in the exposed fish, as indicated by significant inhibition of SOD, CAT and GPx activity, a remarkable decrease in GSH level, and simultaneous elevation of MDA content. These results suggested that the effect of the combined factors on metal accumulation and oxidative stress biomarkers was more obvious than that of single factors at longer exposure durations.

  13. Parasitological and histological analysis of a new species of the genus Thalohanellus and description of a myxozoan parasite (Myxosporea: Bivalvulida from cultured ornamental goldfish, Carassius auratus L.

    Directory of Open Access Journals (Sweden)

    Mandira Saha

    2017-11-01

    Full Text Available An ornamental fish parasitological survey of West Bengal, India during the year 2014–16 revealed that goldfish, Carassius auratus, was the most susceptible species for myxozoan infestation. This communication revealed the presence of two myxosporean species belonging to the genera Myxobolus and Thelohanellus. Although myxozoan infestation has been determined by isolating small to large, spherical to ellipsoidal plasmodia up to 0.5–2.5 mm were filled with disporic pansporoblasts and mature spores. M. ichkeulensis and one new species T. dipaki n. sp. have been isolated infecting the ornamental goldfish (Carassius auratus for the first time in India. In the present study, new host, and new locality for M. ichkeulensis have been reported. The description of M. ichkeulensis is being considered as a first report from India. Spore of T. dipaki n. sp. measures uniquely 13.99 ± 0.60 × 9.82 ± 0.60 μm in size, having a one globular pyriform polar capsule measuring 7.45 ± 0.62 × 5.91 ± 0.39 μm. The severity of newly isolated myxozoan infestation has also been assessed by the histopathological changes of fins of the hostfish. A combination of light and scanning electron microscopic observation along with its severity of infestation, comparison of same and closely related species has been incorporated to identify the new species. The paper deals with the diversity, distribution and taxonomic descriptions of new and known myxozoan species along with new host, locality records and incidence of infestation.

  14. BIOLOGICAL CHARACTERISTICS OF PRUSSIAN CARP (CARASSIUS AURATUS GIBELIO (BLOCH, 1782 COMMERCIAL STOCK OF THE DNIEPER-BUG ESTUARY

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    К. Heina

    2017-09-01

    Full Text Available Purpose. To provide the biological assessment of the silver Prussian carp (Carassius auratus gibelio (Bloch, 1782 commercial stock of the Dnieper-Bug estuary in the conditions of the transformed Dnieper river flow. Methodology. During the analysis of the biological state of the Prussian carp commercial stock, the main attention was given to the dynamics of age and sexual structure, length-weight growth rate, absolute fecundity and condition factor. The basic data were collected during the work of control-observation stations of the Institute of Fisheries in the Dnieper-Bug estuary during the current century. The collection and processing of ichthyological materials were performed in accordance with the generally accepted methodologies. Findings. The analysis showed that during the current century, the age structure of the Prussian carp of the Dnieper-Bug estuary was the most labile among other commercial cyprinids. It was found that as a result of an increase in the right wing of the age series, there was a gradual increase of the mean weighted age of its commercial stock. At the beginning of studies (2001-2002, the core of the stock was formed by age-3-6 fish (up ; however in subsequent years, a displacement of dominant groups toward the dominance of age-4-7 fish (more than 80% of the total stock was observed. At the same time, the relative number of age-3 fish (recruits was at a relatively high level – up to 10.6%. The linear growth varied more intensively until the age-5, but it reduced with ageing and did not show high variability. The body weight most variable was in age-4 fish (Cv=9.62%. The noted insignificant deviations in the body weight growth rate of the right wing of the age series was due to stable predominance of females in the stock structure, which were characterized by a variability of the mean weight as a result of different development of gonads. The dynamics of the age-related changes in the condition factor indicated on a

  15. PERBEDAAN TINGKAT STRES LUTUNG JAWA (Trachypitecus auratus) PADA KANDANG PERAWATAN DAN KANDANG KARANTINA DI JAVAN LANGUR CENTER (JLC) DITINJAU DARI KADAR KORTISOL DAN RASIO NEUTROFIL PERLIMFOSIT (N/L)

    OpenAIRE

    Eko Dini Danafi; Djoko Winarso; Rahadi Swatomo; Ahmad Fauzi; Ida Masnur; Iwan Kurniawan; Nurina Titisari

    2017-01-01

    Lutung Jawa (Trachypithecus auratus) adalah satwa endemik Jawa dan Bali. Populasi lutung Jawa semakin mengalami penurunan dan masuk kategori satwa yang dilindungi. Penurunan populasi lutung jawa dapat disebabkan oleh berbagai macam penyakit yang diperparah dengan tingginya tingkat stres pada satwa. Javan Langur Center yang berlokasi di Cangar, Batu merupakan salah satu tempat untuk merehabilitasi lutung Jawa sebelum dilepasliarkan ke alam. Javan Langur Center terdiri dari tiga kandang yaitu k...

  16. Temperature and diet effect on the pepsin enzyme activities, digestive somatic index and relative gut length of Malabar blood snapper (Lutjanus malabaricus Bloch & Schneider, 1801

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    Sabuj Kanti Mazumder

    2018-02-01

    Full Text Available An integrated experiment was performed on juvenile Malabar blood snapper (Lutjanus malabaricus to investigate the effect of temperature and diet in their pepsin activities in relation with digestive somatic index (IDS and relative gut length (RGL. One hundred twenty L. malabaricus juvenile (13–15 cm were equally distributed among four exposed temperature treatments (22, 26, 30 and 34 °C representing their seasonal range and to account for end of century predicted temperatures, and two diets as commercial pellet and natural shrimp. After 7 days of acclimation period fish were reared for 30 days in twenty four 400 l glass aquaria at a stocking density of 5 fish tank−1. All treatments were three replications. The result showed that, IDS and RGL gradually decreased with increasing temperature up to 30 °C and again increase at 34 °C. And the values were also higher in pellet feeding fish than shrimp feeding fish at all the temperatures. Alternatively, in pepsin activity, an increased activity was seen between 26 °C to 30 °C and this activity was significantly higher than the 22 °C and 34 °C (P < 0.05. In general, highest pepsin activity was observed among fish which fed on a natural shrimp diet reared at temperature 30 °C (5.47 ± 1.60 U mg protein−1, followed by those at 26, 34 and 22 °C (P < 0.05 at both diet however, no mortalities were observed. These results could be used as a basis for selecting a suitable diet for maximizing the growth and sustainable aquaculture coping with global warming.

  17. Modulation of vitellogenin synthesis through estrogen receptor beta-1 in goldfish (Carassius auratus) juveniles exposed to 17-β estradiol and nonylphenol

    International Nuclear Information System (INIS)

    Soverchia, L.; Ruggeri, B.; Palermo, F.; Mosconi, G.; Cardinaletti, G.; Scortichini, G.; Gatti, G.; Polzonetti-Magni, A.M.

    2005-01-01

    Many synthetic chemicals, termed xenoestrogens, have been shown to interact as agonists with the estrogen receptor (ER) to elicit biological responses similar to those of natural hormones. To date, the regulation of vitellogenesis in oviparous vertebrates has been widely used for evaluation of estrogenic effects. Therefore, Carassius auratus juveniles were chosen as a fish model for studying the effects of estradiol-17β and different concentrations (10 -6 and 10 -7 M) of 4-nonylphenol (4-NP) on the expression of liver ERβ-1 subtype; plasma vitellogenin and sex steroids (androgens and estradiol-17β) were also evaluated together with the bioaccumulation process, through mass-spectrometry. C. auratus is a species widespread in the aquatic environment and, on the toxicological point of view, can be considered a good 'sentinel' species. Juveniles of goldfish were maintained in tanks with only tap water or water with different concentrations (10 -6 and 10 -7 M) of 4-nonylphenol (4-NP), or 10 -7 M of estradiol-17β. After 3 weeks of treatment, animals were anesthetized within 5 min after capture, and blood was immediately collected into heparinized syringes by cardiac puncture and stored at -70 deg. C; the gonads were fixed, then frozen and stored at -70 deg. C; the whole fish, liver, and muscle tissues were harvested and immediately stored at -70 deg. C for molecular biology experiments and bioaccumulation measurements. The estrogenic effects of 4-NP were evidenced by the presence of plasma vitellogenin in juveniles exposed both to estradiol-17β and the two doses of 4-NP; moreover, exposure to 4-NP also increased aromatization of androgens, as suggested by decreasing androgens and increasing estradiol-17β plasma levels. The changes of these parameters were in agreement with the increasing transcriptional rate of ERβ-1 mRNA in the liver, demonstrating that both estradiol-17β and 4-NP modulate the vitellogenin rate through interaction with the ERβ-1 subtype. The

  18. Genotoxicity of Water Contaminants from the Basin of Lake Sevan, Armenia Evaluated by the Comet Assay in Gibel Carp (Carassius auratus gibelio) and Tradescantia Bioassays.

    Science.gov (United States)

    Simonyan, Anna; Gabrielyan, Barduch; Minasyan, Seyran; Hovhannisyan, Galina; Aroutiounian, Rouben

    2016-03-01

    Combination of bioassays and chemical analysis was applied to determine the genotoxic/mutagenic contamination in four different sites of the basin of Lake Sevan in Armenia. Water genotoxicity was evaluated using the single cell gel electrophoresis technique (comet assay) in erythrocytes of gibel carp (Carassius auratus gibelio), Tradescantia micronucleus (Trad-MCN) and Tradescantia stamen hair mutation (Trad-SHM) assays. Significant inter-site differences in the levels of water genotoxicity according to fish and Trad-MCN bioassays have been revealed. Two groups of locations with lower (south-southwest of the village Shorzha and Peninsula of Lake Sevan) and higher (estuaries of Gavaraget and Dzknaget rivers) levels of water genotoxicity were distinguished. Correlation analysis support the hypothesis that the observed genetic alterations in fish and plant may be a manifestation of the effects of water contamination by nitrate ions, Si, Al, Fe, Mn and Cu. Increase of DNA damage in fish also correlated with content of total phosphorus.

  19. Effects of melatonin and green-wavelength LED light on the physiological stress and immunity of goldfish, Carassius auratus, exposed to high water temperature.

    Science.gov (United States)

    Jung, Seo Jin; Kim, Na Na; Choi, Young Jae; Choi, Ji Yong; Choi, Young-Ung; Heo, Youn Seong; Choi, Cheol Young

    2016-10-01

    This study investigated the effects of increasing water temperature (22-30 °C) on the physiological stress response and immunity of goldfish, Carassius auratus, and the ability of green light-emitting diode (LED) irradiation or melatonin injections to mitigate this temperature-induced stress. To evaluate the effects of either green-wavelength LED light or melatonin on stress in goldfish, we measured plasma triiodothyronine (T3), thyroxine (T4), and thyroid hormone receptor (TR) mRNA expression; plasma cortisol and glucose; and immunoglobulin M (IgM) and lysozyme mRNA expression. The thyroid hormone activities, TR mRNA expression, and plasma cortisol and glucose were higher in goldfish exposed to high-temperature water, but were lower after exposure to melatonin or green-wavelength LED light. Lysozyme mRNA expression and plasma IgM activity and protein expression were lower after exposure to high water temperatures and higher after melatonin or green-wavelength LED light treatments. Therefore, high water temperature induced stress and decreased immunity; however, green-wavelength LED light and melatonin treatments mitigated the effects of stress and enhanced immunity. The benefits of melatonin decreased with time, whereas those of green-wavelength LED treatment did not.

  20. Effects of melatonin injection or green-wavelength LED light on the antioxidant system in goldfish (Carassius auratus) during thermal stress.

    Science.gov (United States)

    Jung, Seo Jin; Choi, Young Jae; Kim, Na Na; Choi, Ji Yong; Kim, Bong-Seok; Choi, Cheol Young

    2016-05-01

    We tested the mitigating effects of melatonin injections or irradiation from green-wavelength light-emitting diodes (LEDs) on goldfish (Carassius auratus) exposed to thermal stress (high water temperature, 30 °C). The effects of the two treatments were assessed by measuring the expression and activity levels of the antioxidant enzymes, superoxide dismutase and catalase, plasma hydrogen peroxide, lipid hydroperoxide, and lysozyme. In addition, a comet assay was conducted to confirm that high water temperature damaged nuclear DNA. The expression and activity of the antioxidant enzymes, plasma hydrogen peroxide, and lipid hydroperoxide were significantly higher after exposure to high temperature and were significantly lower in fish that received melatonin or LED light than in those that received no mitigating treatment. Plasma lysozyme was significantly lower after exposure to high temperature and was significantly higher after exposure to melatonin or LED light. The comet assay revealed that thermal stress caused a great deal of damage to nuclear DNA; however, treatment with melatonin or green-wavelength LED light prevented a significant portion of this damage from occurring. These results indicate that, although high temperatures induce oxidative stress and reduce immune system strength in goldfish, both melatonin and green-wavelength LED light inhibit oxidative stress and boost the immune system. LED treatment increased the antioxidant and immune system activity more significantly than did melatonin treatment. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Organization and variation analysis of 5S rDNA in gynogenetic offspring of Carassius auratus red var. (♀) × Megalobrama amblycephala (♂).

    Science.gov (United States)

    Qin, QinBo; Wang, Juan; Wang, YuDe; Liu, Yun; Liu, ShaoJun

    2015-03-13

    The offspring with 100 chromosomes (abbreviated as GRCC) have been obtained in the first generation of Carassius auratus red var. (abbreviated as RCC, 2n = 100) (♀) × Megalobrama amblycephala (abbreviated as BSB, 2n = 48) (♂), in which the females and unexpected males both are found. Chromosomal and karyotypic analysis has been reported in GRCC which gynogenesis origin has been suggested, but lack genetic evidence. Fluorescence in situ hybridization with species-specific centromere probes directly proves that GRCC possess two sets of RCC-derived chromosomes. Sequence analysis of the coding region (5S) and adjacent nontranscribed spacer (abbreviated as NTS) reveals that three types of 5S rDNA class (class I; class II and class III) in GRCC are completely inherited from their female parent (RCC), and show obvious base variations and insertions-deletions. Fluorescence in situ hybridization with the entire 5S rDNA probe reveals obvious chromosomal loci (class I and class II) variation in GRCC. This paper provides directly genetic evidence that GRCC is gynogenesis origin. In addition, our result is also reveals that distant hybridization inducing gynogenesis can lead to sequence and partial chromosomal loci of 5S rDNA gene obvious variation.

  2. Accumulation and Toxicity of CuO and ZnO Nanoparticles through Waterborne and Dietary Exposure of Goldfish (Carassius auratus)

    Science.gov (United States)

    Ates, Mehmet; Arslan, Zikri; Demir, Veysel; Daniels, James; Farah, Ibrahim O.

    2014-01-01

    Dietary and waterborne exposure to CuO and ZnO nanoparticles (NPs) was conducted using a simplified model of an aquatic food chain consisting of zooplankton (Artemia salina) and goldfish (Carassius auratus) to determine bioaccumulation, toxic effects and particle transport through trophic levels. Artemia contaminated with NPs were used as food in dietary exposure. Fish were exposed to suspensions of the NPs in waterborne exposure. ICP-MS analysis showed that accumulation primarily occurred in the intestine, followed by the gills and liver. Dietary uptake was lower, but was found to be a potential pathway for transport of NPs to higher organisms. Waterborne exposure resulted in about a tenfold higher accumulation in the intestine. The heart, brain and muscle tissue had no significant Cu or Zn. However, concentrations in muscle increased with NP concentration, which was ascribed to bioaccumulation of Cu and Zn released from NPs. Free Cu concentration in the medium was always higher than that of Zn, indicating CuO NPs dissolved more readily. ZnO NPs were relatively benign, even in waterborne exposure (p≥0.05). In contrast, CuO NPs were toxic. Malondialdehyde levels in the liver and gills increased substantially (p<0.05). Despite lower Cu accumulation, the liver exhibited significant oxidative stress, which could be from chronic exposure to Cu ions. PMID:24860999

  3. Effect of orally administered azadirachtin on non-specific immune parameters of goldfish Carassius auratus (Linn. 1758) and resistance against Aeromonas hydrophila.

    Science.gov (United States)

    Kumar, Saurav; Raman, R P; Pandey, P K; Mohanty, Snatashree; Kumar, Abhay; Kumar, Kundan

    2013-02-01

    Modulation of the immune responses using active bio-ingredients as a possible prophylaxis measure has been novel prospect for aquaculture. The present study evaluated the effects of azadirachtin EC 25% on non-specific immune responses in goldfish Carassius auratus and resistance against pathogenic bacteria Aeromonas hydrophila. The experimental trial for effects of azadirachtin on immuno-haematoloical parameters in goldfish was conducted by feeding the various levels of azadirachtin as control T(0) (without azadirachtin), T(1) (0.1%), T(2) (0.2%), T(3) (0.4%), T(4) (0.8%) and T(5) (1.6%) for a period of 28 days. Fishes were challenged with A. hydrophila 28 days post feeding and relative percentage survival (%) was recorded over 14 days post infection. Immuno-haematoloical (total erythrocyte count, total leukocyte count, haemoglobin, packed cell volume, NBT activity, phagocytic activity, serum lysozyme activity, myeloperoxidase activity, total immunoglobulin) and serum biochemical parameters (serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and blood glucose) of fishes were examined at 14 and 28 days of feedings. Fish fed with azadirachtin, showed significantly (p 0.05) in the treatment groups compared to control groups. Azadirachtin at the concentration of 4 g kg(-1) showed significantly (p azadirachtin EC 25% (4 g kg(-1)) showed higher NBT activity, serum lysozyme, protein profiles, leukocyte counts and resistance against A. hydrophila infection and thus, can be used as a potential immunostimulant in aquaculture. Copyright © 2012 Elsevier Ltd. All rights reserved.

  4. Lectin histochemistry as a tool to identify apoptotic cells in the seminiferous epithelium of Syrian hamster (Mesocricetus auratus) subjected to short photoperiod.

    Science.gov (United States)

    Seco-Rovira, V; Beltrán-Frutos, E; Ferrer, C; Sánchez-Huertas, M M; Madrid, J F; Saez, F J; Pastor, L M

    2013-12-01

    Lectins have been widely used to study the pattern of cellular glycoconjugates in numerous species. In the process of cellular apoptosis, it has been observed that changes occur in the membrane sugar sequences of these apoptotic cells. The aim of our work was to identify which lectins, out of an extensive battery of the same (PNA, SBA, HPA, LTA, Con-A, UEA-I, WGA, DBA, MAA, GNA, AAA, SNA), show affinity for germinal cells in apoptosis, at what stage of cell death they do so and in which germinal cell types they can be detected. For this, we studied testis sections during testicular regression in Syrian hamster (Mesocricetus auratus) subjected to short photoperiod. Several lectins showed an affinity for the glycoconjugate residues of germ cells in apoptosis: Gal β1,3-GalNAcα1, α-d-mannose, N-acetylgalactosamine and l-fucose. Furthermore, lectin specificity was observed for some specific germinal cells and in certain stages of apoptosis. It was also observed that one of these lectins (PNA) showed affinity for Sertoli cells undergoing apoptosis. Therefore, we conclude that the use of lectin histochemistry could be a very useful tool for studying apoptosis in the seminiferous epithelium because of the specificity shown towards germinal cells in pathological or experimentally induced epithelial depletion models. © 2013 Blackwell Verlag GmbH.

  5. Yellow fever virus isolated from a fatal post vaccination event: an experimental comparative study with the 17DD vaccine strain in the Syrian hamster (Mesocricetus auratus

    Directory of Open Access Journals (Sweden)

    Sueli Guerreiro Rodrigues

    2004-01-01

    Full Text Available In order to investigate the pathogenicity of the virus strain GOI 4191 that was isolated from a fatal adverse event after yellow fever virus (YFV vaccination, an experimental assay using hamsters (Mesocricetus auratus as animal model and YFV 17DD vaccine strain as virus reference was accomplished. The two virus strains were inoculated by intracerebral, intrahepatic and subcutaneous routes. The levels of viremia, antibody response, and aminotransferases were determined in sera; while virus, antigen and histopathological changes were determined in the viscera. No viremia was detected for either strain following infection; the immune response was demonstrated to be more effective to strain GOI 4191; and no significant aminotransferase levels alterations were detected. Strain GOI 4191 was recovered only from the brain of animals inoculated by the IC route. Viral antigens were detected in liver and brain by immunohistochemical assay. Histothological changes in the viscera were characterized by inflammatory infiltrate, hepatocellular necrosis, and viral encephalitis. Histological alterations and detection of viral antigen were observed in the liver of animals inoculated by the intrahepatic route. These findings were similar for both strains used in the experiment; however, significant differences were observed from those results previously reported for wild type YFV strains.

  6. Comparative analysis of differential gene expression in kidney tissues of moribund and surviving crucian carp (Carassius auratus gibelio) in response to cyprinid herpesvirus 2 infection.

    Science.gov (United States)

    Xu, Lijuan; Podok, Patarida; Xie, Jun; Lu, Liqun

    2014-08-01

    Cyprinid herpesvirus 2 (CyHV-2) has recently been associated with high mortality of cultured crucian carp (Carassius auratus gibelio) in eastern China. In this study, we established a real-time PCR method to confirm viral infection of crucian carp and to quantify CyHV-2 particles obtained by sucrose gradient centrifugation from diseased fish. Virus-free crucian carp were artificially infected with CyHV-2 using an injection method, which resulted in a dose-dependent death rate. In situ hybridization analysis indicated that there was extensive viral replication and lysis in the kidneys of moribund fish, in contrast to very limited replication in surviving fish. To probe the host immune response to viral infection at the level of gene expression, we identified virus-responsive genes using suppression subtractive hybridization (SSH) in head kidney tissues, the principal immune organ of fish, from moribund and surviving crucian carps after viral challenge. From the moribund SSH library, 363 expressed sequence tags (ESTs) were clustered to 234 unigenes (including 15 singletons and 45 contigs). From the survivor SSH library, 599 ESTs was clustered to 549 unigenes (including 107 singletons and 105 contigs). We further analyzed the transcriptional levels of all immune-related genes by quantitative real-time RT-PCR, which confirmed the upregulation of 90.48 % of these genes. The significantly upregulated immune-related genes identified in this study can serve as candidate marker genes for acute CyHV-2 infection.

  7. Detection of cyprinid herpesvirus 2 in peripheral blood cells of silver crucian carp, Carassius auratus gibelio (Bloch), suggests its potential in viral diagnosis.

    Science.gov (United States)

    Wang, H; Xu, Lj; Lu, Lq

    2016-02-01

    Epidemics caused by cyprinid herpesvirus 2 (CyHV-2) in domestic cyprinid species have been reported in both European and Asian countries. Although the mechanisms remain unknown, acute CyHV-2 infections generally result in high mortality, and the surviving carps become chronic carriers displaying no external clinical signs. In this study, in situ hybridization analysis showed that CyHV-2 tended to infect peripheral blood cells during either acute or chronic infections in silver crucian carp, Carassius auratus gibelio (Bloch). Laboratory challenge experiments coupled with real-time PCR quantification assays further indicated that steady-state levels of the viral genomic copy number in fish serum exhibited a typical 'one-step' growth curve post-viral challenge. Transcriptional expression of open reading frames (ORF) 121, which was selected due to its highest transcriptional levels in almost all tested tissues, was monitored to represent the replication kinetics of CyHV-2 in peripheral blood cells. Similar kinetic curve of active viral gene transcription in blood cells was obtained as that of serum viral load, indicating that CyHV-2 replicated in peripheral blood cells as well as in other well-characterized tissues. This study should pave the way for designing non-invasive and cost-effective serum diagnostic methods for quick detection of CyHV-2 infection. © 2015 John Wiley & Sons Ltd.

  8. Development of the leptospirosis by experimental infection in hamsters (Mesocricetus auratus with Leptospira interrogans serovar Canicola, strain LO4, by intact and scratched skin exposures

    Directory of Open Access Journals (Sweden)

    Carolina de Sousa Américo Batista

    2010-10-01

    Full Text Available The establishment and evolution of leptospirosis in hamster (Mesocricetus auratus by experimental infection with Leptospira interrogans serovar Canicola, LO4 strain, by intact and scratched skin exposures, having as control the intraperitoneal route, were evaluated. Hundred-twenty female hamsters distributed in two groups according to inoculation route (intact and scratched skin were used. Infectious inoculum was constituted by a pure culture of L. interrogans serovar Canicola (strain LO4, isolated from liver from a slaughtered swine in Londrina, Paraná state and typified by agglutinins adsortion technique with monoclonal antibody kit at the Royal Tropical Institute, Amsterdam, the Netherlands. The animals were observed twice a day during 21 days. Animals that died were necropsied and kidneys, liver, genital tract (uterus and ovaries and brain were aseptically collected. On the 21st post-inoculation day, surviving animals were euthanized. In these animals, serum samples were also collected by cardiac puncture to antileptospires agglutinins research using microscopic agglutination test (MAT. Fresh direct microscopy and microbiological culture were used for the detection of leptospires. Scratched skin route induced larger lethality when compared to intact skin route, with establishment and evolution of leptospirosis. On the other hand, intact skin route induced renal and/or genital carrier state more frequently. LO4 strain presented low immunogenic power, characterized by soroconversion at the MAT in only one inoculated animal.

  9. Influence of the diet on the microbial diversity of faecal and gastrointestinal contents in gilthead sea bream (Sparus aurata) and intestinal contents in goldfish (Carassius auratus).

    Science.gov (United States)

    Silva, Flávia Cristina de Paula; Nicoli, Jacques Robert; Zambonino-Infante, José Luiz; Kaushik, Sadasivam; Gatesoupe, François-Joël

    2011-11-01

    Fish intestinal microbiota changes with the diet and this effect is of particular interest considering the increasing substitution of fish meal by plant protein sources. The objective of this work was to study the effects of partial substitution of fish meal with lupin and rapeseed meals on gut microbiota of the gilthead sea bream (Sparus aurata) and in goldfish (Carassius auratus). Faecal, gastrointestinal and intestinal contents were characterized using culture-based and molecular methods. Vibrionaceae was high in faeces and in the intestine of sea bream, while a more diverse microbiota was retrieved from the stomach, where Bacillales and Flavobacteriaceae appeared to be influenced by the diet. PCR-denaturing gradient gel electrophoresis profiles revealed a high diversity of the microbiota transiting in the sea bream digestive tract, with a shift between gastric and intestinal communities, especially in the group fed with lupin meal. The goldfish was different, with a predominance of Aeromonas spp., Shewanella putrefaciens and Staphylococcus spp. among the aerotolerant-cultivable bacteria. The culture-independent methods revealed the presence of anaerobes like Cetobacterium somerae, and that of Vibrio spp., likely in a viable, but noncultivable state. There was a trend towards decreasing diversity in goldfish microbiota with the partial substitution by lupin, which seemed to inhibit some taxa. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  10. Waterborne gemfibrozil challenges the hepatic antioxidant defense system and down-regulates peroxisome proliferator-activated receptor beta (PPARβ) mRNA levels in male goldfish (Carassius auratus)

    International Nuclear Information System (INIS)

    Mimeault, C.; Trudeau, V.L.; Moon, T.W.

    2006-01-01

    The lipid regulator gemfibrozil (GEM) is one of many human pharmaceuticals found in the aquatic environment. We previously demonstrated that GEM bioconcentrates in blood and reduces plasma testosterone levels in goldfish (Carassius auratus). In this study, we address the potential of an environmentally relevant waterborne concentration of GEM (1.5 μg/l) to induce oxidative stress in goldfish liver and whether this may be linked to GEM acting as a peroxisome proliferator (PP). We also investigate the autoregulation of the peroxisome proliferator-activated receptors (PPARs) as a potential index of exposure. The three PPAR subtypes (α, β, and γ) were amplified from goldfish liver cDNA. Goldfish exposed to a concentration higher (1500 μg/l) than environmentally relevant for 14 and 28 days significantly reduce hepatic PPARβ mRNA levels (p < 0.001). Levels of CYP1A1 mRNA were unchanged. GEM exposure significantly induced the antioxidant defense enzymes catalase (p < 0.001), glutathione peroxidase (p < 0.001) and glutathione-S-transferase (p = 0.006) but not acyl-CoA oxidase or glutathione reductase. As GEM exposure failed to increase levels of thiobarbituric reactive substances (TBARS), we conclude that a sub-chronic exposure to GEM upregulates the antioxidant defense status of the goldfish as an adaptive response to this human pharmaceutical

  11. Phylogeny of deepwater snappers (Genus Etelis) reveals a cryptic species pair in the Indo-Pacific and Pleistocene invasion of the Atlantic.

    Science.gov (United States)

    Andrews, Kimberly R; Williams, Ashley J; Fernandez-Silva, Iria; Newman, Stephen J; Copus, Joshua M; Wakefield, Corey B; Randall, John E; Bowen, Brian W

    2016-07-01

    Evolutionary genetic patterns in shallow coastal fishes are documented with dozens of studies, but corresponding surveys of deepwater fishes (>200m) are scarce. Here we investigate the evolutionary history of deepwater snappers (genus Etelis), comprised of three recognized Indo-Pacific species and one Atlantic congener, by constructing a phylogeny of the genus with two mtDNA loci and two nuclear introns. Further, we apply range-wide Indo-Pacific sampling to test for the presence and distribution of a putative cryptic species pair within E. carbunculus using morphological analyses and mtDNA cytochrome b sequences from 14 locations across the species range (N=1696). These analyses indicate that E. carbunculus is comprised of two distinct, non-interbreeding lineages separated by deep divergence (d=0.081 in cytochrome b). Although these species are morphologically similar, we identified qualitative differences in coloration of the upper-caudal fin tip and the shape of the opercular spine, as well as significant differences in adult body length, body depth, and head length. These two species have overlapping Indo-Pacific distributions, but one species is more widespread across the Indo-Pacific, whereas the other species is documented in the Indian Ocean and Western Central Pacific. The dated Etelis phylogeny places the cryptic species divergence in the Pliocene, indicating that the biogeographic barrier between the Indian and Pacific Oceans played a role in speciation. Based on historic taxonomy and nomenclature, the species more widespread in the Pacific Ocean is E. carbunculus, and the other species is previously undescribed (referred to here as E. sp.). The Atlantic congener E. oculatus has only recently (∼0.5Ma) diverged from E. coruscans in the Indo-Pacific, indicating colonization via southern Africa. The pattern of divergence at the Indo-Pacific barrier, and Pleistocene colonization from the Indian Ocean into the Atlantic, is concordant with patterns observed

  12. A High-Density Genetic Linkage Map and QTL Fine Mapping for Body Weight in Crucian Carp (Carassius auratus Using 2b-RAD Sequencing

    Directory of Open Access Journals (Sweden)

    Haiyang Liu

    2017-08-01

    Full Text Available A high-resolution genetic linkage map is essential for a wide range of genetics and genomics studies such as comparative genomics analysis and QTL fine mapping. Crucian carp (Carassius auratus is widely distributed in Eurasia, and is an important aquaculture fish worldwide. In this study, a high-density genetic linkage map was constructed for crucian carp using 2b-RAD technology. The consensus map contains 8487 SNP markers, assigning to 50 linkage groups (LGs and spanning 3762.88 cM, with an average marker interval of 0.44 cM and genome coverage of 98.8%. The female map had 4410 SNPs, and spanned 3500.42 cM (0.79 cM/marker, while the male map had 4625 SNPs and spanned 3346.33 cM (0.72 cM/marker. The average recombination ratio of female to male was 2.13:1, and significant male-biased recombination suppressions were observed in LG47 and LG49. Comparative genomics analysis revealed a clear 2:1 syntenic relationship between crucian carp LGs and chromosomes of zebrafish and grass carp, and a 1:1 correspondence, but extensive chromosomal rearrangement, between crucian carp and common carp, providing evidence that crucian carp has experienced a fourth round of whole genome duplication (4R-WGD. Eight chromosome-wide QTL for body weight at 2 months after hatch were detected on five LGs, explaining 10.1–13.2% of the phenotypic variations. Potential candidate growth-related genes, such as an EGF-like domain and TGF-β, were identified within the QTL intervals. This high-density genetic map and QTL analysis supplies a basis for genome evolutionary studies in cyprinid fishes, genome assembly, and QTL fine mapping for complex traits in crucian carp.

  13. Tooth Morphogenesis and FGF4 Expression During Development of Molar Tooth in Three Muroid Rodents: Calomyscus elburzensis (Calomyscidae), Mesocricetus auratus (Cricetidae) and Mus musculus (Muridae).

    Science.gov (United States)

    Hamidi, Kordiyeh; Darvish, Jamshid; Matin, Maryam M; Javanmard, Athar Sadat; Kilpatrick, C William

    2017-12-01

    To date, no studies have examined the tooth formation during developmental stages of brush-tailed mice (Calomyscidae) and true hamsters (Cricetidae). Herein, we compared the timing of tooth morphogenesis and FGF4 expression pattern during development of the first lower molar in Goodwin's brush-tailed mouse, Calomyscus elburzensis with two other muroid rodents; the house mouse, Mus musculus (Muridae), model organism for tooth morphogenesis, and the golden hamster, Mesocricetus auratus which shares great similarities in cusp pattern with brush-tailed mice. All three species were bred in captivity and developing embryos were isolated at different embryonic days (E). Histological evaluation of lower molars was performed and spatiotemporal pattern of FGF4 expression was determined by immunohistochemistry. Results indicated that morphogenesis of the tooth cusps starts at the beginning of the cap stage of the first lower molar (E14 in house mouse, about E11.5 in golden hamster and E22 in Goodwin's brush-tailed mouse). During the cap to bell stage (E15 in house mouse, E12 in golden hamster and at about E24 in Goodwin's brush-tailed mouse), a decrease in the expression of FGF4 was observed in the mesenchyme, except for the cusp tips. According to our observations, the developmental process of the first lower molar formation in Goodwin's brush-tailed mouse began much later as compared with the other two species. Despite the differences in the temporal pattern of molar development between these three members of the same superfamily (Muroidea), the correlation in the expression of FGF4 with specific stages of tooth morphogenesis supported its regulatory function. Anat Rec, 300:2138-2149, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  14. Comparative antioxidant status in freshwater fish Carassius auratus exposed to six current-use brominated flame retardants: A combined experimental and theoretical study

    Energy Technology Data Exchange (ETDEWEB)

    Feng, Mingbao; Qu, Ruijuan; Wang, Chao; Wang, Liansheng; Wang, Zunyao, E-mail: wangzun315cn@163.com

    2013-09-15

    Highlights: •A combined experimental and theoretical approach was used for risk assessments of six BFRs in fish. •Oxidative stress biomarkers were measured for toxicity identification. •Toxicity order was proposed via the integrated biomarker response. •Theoretical calculations were performed to analyze the BFRs toxicity. -- Abstract: Decabromodiphenyl ether (BDE-209) and several non-polybrominated diphenyl ether (PBDE) brominated flame retardants (BFRs), such as tetrabromobisphenol A (TBBPA), hexabromocyclododecane (HBCD), decabromodiphenyl ethane (DBDPE), hexabromobenzene (HBB) and pentabromotoluene (PBT), are persistent halogenated contaminants ubiquitously detected in aquatic systems. However, data on comparative toxicological effects of these BFRs are lacking for fish. In this study, a combined experimental and theoretical approach was used to compare and analyze the effects of these BFRs on biochemical biomarkers in liver of Carassius auratus injected intraperitoneally with different doses (10 and 100 mg/kg) for 7, 14 and 30 days. Oxidative stress was evoked evidently for the prolonged exposure, represented by the significantly altered indices (superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione, and malondialdehyde). The integrated biomarker response (IBR) index ranked biotoxicity as: PBT > HBB > HBCD > TBBPA > BDE-209 > DBDPE. Quantum chemical calculations (electronic parameters, frontier molecular orbitals, and Wiberg bond order) were performed for theoretical analysis. Notably, some descriptors were correlated with the toxicity order, probably implying the existence of a potential structure–activity relationship when more BFRs were included. Besides, theoretical calculations also provided some valuable information regarding the molecular characteristics and metabolic pathways of these current-use BFRs, which may facilitate the understanding on their environmental behavior and fate. Overall, this study adopted a combined

  15. Alternations in neuroendocrine and endocrine regulation of reproduction in male goldfish (Carassius auratus) following an acute and chronic exposure to vinclozolin, in vivo.

    Science.gov (United States)

    Golshan, Mahdi; Hatef, Azadeh; Zare, Ava; Socha, Magdalena; Milla, Sylvain; Gosiewski, Grzegorz; Fontaine, Pascal; Sokołowska-Mikołajczyk, Mirosława; Habibi, Hamid R; Alavi, Sayyed Mohammad Hadi

    2014-10-01

    The fungicide vinclozolin (VZ) is in use globally and known to disrupt reproductive function in male. The present study tested the hypothesis that VZ disrupts testicular function in goldfish (Carassius auratus) by affecting brain-pituitary-testis axis. Goldfish were exposed to 100, 400 and 800 μg/L VZ and 5 μg/L 17β-estradiol (E2) for comparison. In VZ treated goldfish, 11-ketotesteosterone (11-KT) secretion was changed depending on dose and duration period of treatment. Following 7 days of exposure, 11-KT was decreased in goldfish exposed to 800 μg/L VZ, while it was increased in goldfish exposed to 100 μg/L VZ after 30 days of exposure. Circulating E2 level was unchanged in VZ treated goldfish, however the E2/11-KT ratio was increased in a concentration-related manner. In E2 treated goldfish, circulatory 11-KT and E2 levels were decreased and increased, respectively, which resulted in an increase in the E2/11-KT ratio. Exposure to VZ at 100 μg/L caused a significant increase in the circulatory luteinizing hormone (LH) after 30 days. In E2 treated fish circulatory LH was decreased, significantly. Transcripts of genes encoding gonadotropin-releasing hormone and androgen receptor in the brain, and those of genes encoding LH and follicle-stimulating hormone receptors, StAR, CYP17, and 3β-HSD in the testis changed in VZ-treated goldfish depending on concentration and period of treatment. mRNA of genes encoding vitellogenin and estrogen receptor in the liver and cytochrome P450 aromatase in the brain were increased in E2-treated goldfish. The results suggest that VZ-induced changes in 11-KT were due to disruption in brain-pituitary-testis axis and provide integrated characterization of VZ-related reproductive disorders in male fish. Copyright © 2014 Elsevier B.V. All rights reserved.

  16. Melatonin membrane receptor (MT1R) expression and nitro-oxidative stress in testis of golden hamster, Mesocricetus auratus: An age-dependent study.

    Science.gov (United States)

    Mukherjee, Arun; Haldar, Chandana

    2015-09-01

    Age-dependent decline in melatonin level induces nitro-oxidative stress that compromises physiological homeostasis including reproduction. However, less information exist regarding the age-dependent variation in local melatonin (lMel) concentration and MT1R expression in testis and its interaction with testicular steroidogenesis and nitro-oxidative stress in golden hamster, Mesocricetus auratus. Therefore, we evaluated lMel level along with MT1R expression and its possible interaction with steroidogenesis and nitro-oxidative stress in testes of young (6weeks), adult (15weeks) and old (2years) aged hamsters. Further, we injected the old hamsters with melatonin to address whether age-related decline in lMel and MT1R is responsible for the reduction in testicular steroidogenesis and antioxidant status. Increased expression of steroidogenic markers suggests increased testicular steroidogenesis in adult hamsters that declined in old hamsters. An age-dependent elevation in the level of NOX, TBARS, corticosterone and the expression of iNOS and GR with a concomitant decrease in enzyme activities for SOD, CAT, GSH-PX indicate increased nitro-oxidative stress in testes. Data suggest that reproductive senescence in male hamsters might be a consequence of declined lMel concentration with MT1R expression inducing nitro-oxidative stress resulting in diminished testicular steroidogenesis. However, administration of Mel in old-aged hamsters significantly increased steroidogenesis and antioxidant status without a significant variation in lMel concentration and MT1R expression in testes. Therefore, decreased lMel and MT1R might not be the causative factor underlying the age-associated decrease in antioxidant defence and steroidogenesis in testes. In conclusion, Mel induced amelioration of testicular oxidative insult and elevation of steroidogenic activity suggests a potential role of increased nitro-oxidative stress underlying the age-dependent decrease in steroidogenesis. Copyright

  17. Réponses comportementales du brochet (Esox lucius L. à un extrait épidermique de carassin doré (Carassius auratus L. en olfactomètre

    Directory of Open Access Journals (Sweden)

    SAGLIO Ph.

    1993-04-01

    Full Text Available Les effets de solutions brutes (EE et d'une fraction d'acides aminés libres reconstituée (FAA, issues d'un extrait épidermique de Carassin doré (Carassius auratus L., ont été analysés sur l'attraction et le comportement exploratoire du Brochet (Esox lucius L. en olfactomètre. Des effets significatifs ont été observés en réponse aux différentes solutions et doses testées (EE = 0,005 ; 0,05 ; 0,5 équivalent-carassin/l ; FAA = 0,5 équivalent-carassin/l. L'effet stimulant des solutions épidermiques brutes sur l'exploration varie en raison inverse de la concentration testée. L'activité de ces solutions sur l'attraction augmente avec la concentration. L'effet stimulant de la fraction d'acides aminés libres reconstituée sur l'exploration et sur l'attraction est apparu inférieur à celui observé en réponse à la solution brute. Ces résultats sont discutés à la lueur de données sur le rôle des sécrétions de la peau et des acides aminés dans la communication chimique chez les poissons.

  18. Size-relative Effectiveness of Clove Oil as an Anaesthetic for Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792 and Goldfish (Carassius auratus Linnaeus, 1758

    Directory of Open Access Journals (Sweden)

    Costas Perdikaris

    2010-01-01

    Full Text Available The purpose of this work was to investigate the size-relative effectiveness of clove oil as an anaesthetic for rainbow trout and goldfish. In total, 128 rainbow trout (Oncorhynchus mykiss (two groups of 20-23 and 30-33 cm mean fork length and 160 goldfish (Carassius auratus (four size groups of 1.5-2.5, 5-7, 11-15 and 20-25 cm were anaesthetized at different clove oil concentrations of 50, 100, 150 mg·l-1 for trouts and 75, 100, 150 mg·l-1 for goldfish. Rainbow trout exhibited total loss of balance and no response to external stimuli with shorter induction time as dosage increased (120.5 s, 64.4 s and 44.3 s, respectively. Goldfish exhibited total loss of balance and no response to external stimuli after induction time that varied with dosage used and body size of fish. The small fish (1.5-7 cm exhibited shorter induction time which ranged from 84.28 s at 75 mg·l-1 clove oil to 41.14 s at 150 mg·l-1 clove oil. The larger fish had a longer induction time inversely related to the dosage. Recovery time was longer than induction time in both species. Both species recovered within 6 min after anaesthesia at 150 mg·l-1 clove oil. Clove oil did not produce marked changes (P compared to the control. However, marked changes (P P > 0.05. For both fish species, clove oil was effective, producing minimum stress and zero mortalities, and can be recommended as an effective anaesthetic.

  19. Hamster (Mesocricetus auratus cheek pouch as an experimental model to investigate human skin and keloid heterologous graft Bolsa jugal no hamster (Mesocricetus auratus como modelo experimental de investigação de enxertos heterólogos de pele humana e quelóide

    Directory of Open Access Journals (Sweden)

    Bernardo Hochman

    2004-12-01

    Full Text Available To describe the integration process of grafts of total human skin and keloid in hamster (Mesocricetus auratus cheek pouch, whose sub-epithelium is naturally an "Immunologically Privileged Site". Fragments of human normal skin and keloid from the breast region of mulatto female patients were transplanted into the cheek pouch subepithelium in situ. Surgical procedure and grafted pouches for microscopic exam at several time points of the transplantation were standardized. The integration of grafted fragments of human skin and keloid was seen in late periods (84 days since the microscopic assessment showed the presence of blood vases within the conjunctive tissue of grafted fragments. It was also possible to see among the grafted fragments the epithelium, the appearing of early cellular infiltrated, epithelial secretion of keratin, the presence of melanocytes, and delayed changes on the aspect of collagen fibers of conjunctive tissue. Pooled results allow to define hamster cheek pouch sub-epithelium as an experimental model to investigating heterologous graft physiology of human total skin and keloid with epithelium.Descrever a integração dos enxertos de pele total humana e quelóide na bolsa jugal do hamster (Mesocricetus auratus, cujo subepitélio é, naturalmente, um "Local de Privilégio Imunológico". Foram transplantados fragmentos, de pele humana normal e de quelóide, obtidos da região mamária de pacientes pardas, no subepitélio da bolsa jugal in situ. O procedimento operatório, e de preparo das bolsas enxertadas para exame microscópico em vários períodos de transplante, foi padronizado. Verificou-se a integração dos fragmentos enxertados de pele humana e de quelóide em períodos tardios (84 dias, uma vez que a avaliação microscópica revelou a presença de vasos sangüíneos no tecido conjuntivo dos fragmentos enxertados. Foi também possível observar, nos fragmentos enxertados, o epitélio, o aparecimento de infiltrado

  20. Plâncton, Artemia sp, dieta artificial e suas combinações no desenvolvimento e sobrevivência do quinguio (Carassius auratus durante a larvicultura Plankton, Artemia sp, artificial diet and their combinations on the development and survival of goldfish (Carassius auratus during larviculture phase

    Directory of Open Access Journals (Sweden)

    Eliana Maria Galdioli

    2000-05-01

    Full Text Available O objetivo deste estudo foi verificar a influência do uso de diferentes alimentos sobre o desenvolvimento de larvas de quinguio (Carassius auratus, assim como determinar o melhor manejo alimentar. O delineamento experimental utilizado foi o inteiramente casualizado com cinco tratamentos e quatro repetições. Para análise estatística utilizou-se o teste de Tukey. Utilizaram-se 20 aquários com capacidade para 10 L, sendo estocadas 20 larvas com 5,20 mm de comprimento por aquário. Os tratamentos utilizados foram: somente ração com 56,00% de proteína bruta (R; plâncton (PL; náuplios de Artemia sp (NA, ração + plâncton (R+PL e ração + náuplios de Artemia sp (R+NA, sendo seu fornecimento à vontade duas vezes ao dia. Houve mortalidade de todos os indivíduos submetidos ao tratamento R. O uso de NA e R+NA proporcionou taxas de sobrevivência superiores (p 0,05. Conclui-se que as diferentes fontes de alimento interferem no desempenho das larvas, sendo o manejo alimentar com o fornecimento de ração + náuplios de Artemia sp o mais adequado para a larvicultura do quinguioThe aim of this study was to verify the influence of different diets on the development of goldfish (Carassius auratus larvae and determine the best diet management during its larviculture phase. The experimental design was totally randomized with five treatments and four replicatess, using Tukey test for statistical analysis. Twenty 10L-aquariums were used, where twenty larvae with 5.20 mm length were stocked per aquarium. Quinguio larvae were fed daily ration of different diets: ration alone including 56.00% crude protein (R, plankton (PL, Artemia sp nauplii (AN, ration + plankton (R+PL and ration + Artemia sp nauplii (R+AN, ad libitum twice a day. Treatment R caused the death of all individuals. AN and R+AN diets provided a higher survival rate (p 0.05 was observed between the different treatments. Under the conditions of the present experiment, the results led to

  1. Técnica para obtenção do aparelho geniturinário e dosagem do PSA (Prostate Specific Antigen no hamster sírio, Mesocricetus auratus Technique for collecting blood for PSA (Prostate Specific Antigen dosing and genitourinary system obtaining in syrian hamster, Mesocricetus auratus

    Directory of Open Access Journals (Sweden)

    Dimas José Araújo Vidigal

    2004-12-01

    Full Text Available Objetivo: Expor a técnica utilizada na colheita de sangue para dosagem do PSA ( Prostate Specific Antigen e retirada do aparelho geniturinário no hamster sírio, Mesocricetus auratus, e correlacionar os achados do PSA com as alterações histológicas dos anexos sexuais desse roedor. Métodos: Foram usados no experimento trinta (n= 30 Hamsters: dez (n=10 animais considerados jovens com idade média no momento da morte de 47,5 dias e vinte (n=20 animais considerados adultos com idade superior à um ano. Após serem anestesiados com cloridrato de quetamina e diazepam, foi colhido diretamente da veia cava, em nível de abdome superior, cerca de 1,5mL a 2,0mL de sangue para dosagem do PSA totalpelo método ELISA, com antígeno humano. Morriam após colheita do sangue. Constatado a morte do animal, fazia-se a laparotomia retirando-se em monobloco todo aparelho geniturinário para estudo histológico dos anexos sexuais. Correlacionou-se o PSA com as alterações histológicas encontradas. Resultados: Os animais após serem anestesiados com cloridrato de quetamina e diazepam intraperitonealmente, obteve-se um excelente plano anestésico, que possibilitou colher via trans-dérmica da veia cava inferior em abdome superior sangue para dosagem do PSA. O animal morria após colheita do sangue. Fazia-se a laparotomia, com retirada em monobloco do aparelho geniturinário para estudo histológico e comparação das alterações encontradas nos anexos sexuais com o PSA dosado. Entre os Hamsters Jovens a média do PSA encontrado foi de 0,252ng/mL. Desvio Padrão (DP = 0,36. Entre os Hamsters adultos esta média foi de 0,325 ng/mL, DP= 0,12 . Quando comparou-se as médias do PSA entre os dois grupos de jovens e adultos obteve-se p= 0,0427. Dentre os Hamsters jovens, três apresentaram PSA não detectado. A análise histológica mostrou que, entre os animais jovens, não foi identificada qualquer alteração das estruturas microscópicas da próstata, ves

  2. Operation TUMBLER-SNAPPER 1952

    Science.gov (United States)

    1982-06-14

    coordinates are used in this report, as seen in table 1-1. The first three digits refer to I point on an east-west axis, and the second throe digits ...8217icultural ,ech S’tte ’No,. a4inlovvi as t ;’ib i Libary U ’ aerSji ’, T’" Lbra ": 2N L ibrn "Ne•w 5e,•t ’tall I it’ra ’vr Vn Ver t 1 .o0 ,rthl ’a l

  3. Integração do enxerto heterólogo de pele humana no subepitélio da bolsa jugal do hamster (Mesocricetus auratus

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    Hochman Bernardo

    2003-01-01

    Full Text Available OBJETIVO: Descrever a integração de enxertos de pele total humana no subepitélio da bolsa jugal do hamster (Mesocricetus auratus. MÉTODOS: A amostragem consistiu de 18 hamsters machos, exogâmicos, com 10 a 14 semanas de idade. Fragmentos de pele humana normal foram obtidos de pele excedente de mastoplastia redutora de paciente parda. Cada hamster foi enxertado em ambas as bolsas com fragmentos de pele, perfazendo um total de 36 fragmentos enxertados. Os animais foram distribuídos, em 6 grupos, para exame dos fragmentos enxertados com 5, 12, 21, 42, 84 e 168 dias. Uma avaliação macroscópica foi realizada comparando a bolsa contendo o fragmento enxertado em cada período com a mesma bolsa no pós-operatório imediato, mediante fotografias padronizadas. Na avaliação microscópica foi adotado como critério de integração a presença de vasos sangüíneos na derme dos enxertos. Observou-se também a presença de queratina, melanócitos, infiltrado celular e aspecto do tecido conjuntivo. RESULTADOS: Na avaliação macroscópica foi observada uma reação vascular em torno dos fragmentos até 12 dias do implante, e a presença de pigmentação castanho-escura a partir de 42 dias. À microscopia, integraram-se 80,64% dos fragmentos enxertados, inclusive no grupo de 168 dias. Observou-se infiltrado celular inflamatório até 12 dias, a presença de melanócitos a partir de 42 dias e uma hialinização do tecido conjuntivo após 84 dias. CONCLUSÕES: Fragmentos de pele humana integram-se no tecido celular subcutâneo da bolsa jugal do hamster, mantêm-se vascularizados por 168 dias, e conservam o epitélio íntegro até 21 dias. O subepitélio da bolsa representa modelo experimental de investigação da fisiologia de pele humana ex vivo.

  4. The effects of dissolved organic matter and feeding on bioconcentration and oxidative stress of ethylhexyl dimethyl p-aminobenzoate (OD-PABA) to crucian carp (Carassius auratus).

    Science.gov (United States)

    Ma, Binni; Lu, Guanghua; Yang, Haohan; Liu, Jianchao; Yan, Zhenhua; Nkoom, Matthew

    2018-03-01

    Bioconcentration of UV filters in organisms is an important indicator for the assessment of environmental hazards. However, bioconcentration testing rarely accounts for the influence of natural aquatic environmental factors. In order to better assess the ecological risk of organic UV filters (OUV-Fs) in an actual water environment, this study determined the influences of dissolved organic matter (DOM) (0, 1, 10, and 20 mg/L) and feeding (0, 0.5, 1, and 2% body weight/d) on bioconcentration of ethylhexyl dimethyl p-aminobenzoate (OD-PABA) in various tissues of crucian carp (Carassius auratus). Moreover, oxidative stress in the fish liver caused by the OD-PABA was also investigated by measuring activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST), and levels of glutathione (GSH) and malondialdehyde (MDA). The bioconcentration of OD-PABA in the fish tissues was significantly decreased with the presence of DOM indicating a reduction of OD-PABA bioavailability caused by DOM. The bioconcentration factors (BCFs) decreased by 28.00~50.93% in the muscle, 72.67~96.74% in the gill, 37.84~87.72% in the liver, and 10.32~79.38% in the kidney at different DOM concentrations compared to those of the non-DOM treatments. Significant changes in SOD, CAT, GST, GSH, and MDA levels were found in the DOM- and OD-PABA-alone treatments. However, there were no significant differences in the SOD, CAT, GST, and MDA levels found when co-exposure to OD-PABA and DOM. Feeding led to lower OD-PABA concentrations in the fish tissues, and the concentrations were decreased with increasing feeding ratios. BCFs in various tissues reduced by 39.75~72.52% in the muscle, 56.86~79.73% in the gill, 66.41~87.50% in the liver, and 75.88~89.10% in the kidney, respectively. In the unfed treatments, the levels of SOD and MDA were significantly higher than those of the fed ones while GST and GSH levels were remarkably inhibited indicating the enhanced effect of starvation

  5. Interactive effects of high stocking density and food deprivation on carbohydrate metabolism in several tissues of gilthead sea bream Sparus auratus.

    Science.gov (United States)

    Sangiao-Alvarellos, Susana; Guzmán, José M; Láiz-Carrión, Raúl; Míguez, Jesús M; Martín Del Río, María P; Mancera, Juan M; Soengas, José L

    2005-09-01

    The influence of high stocking density (HSD) and food deprivation was assessed on carbohydrate metabolism of several tissues of gilthead sea bream Sparus auratus for 14 days. Fish were randomly assigned to one of four treatments: (1) fed fish under normal stocking density (NSD) (4 kg m(-3)); (2) fed fish under HSD (70 kg m(-3)); (3) food-deprived fish under NSD; and (4) food-deprived fish under HSD. After 14 days, samples were taken from the plasma, liver, gills, kidney and brain for the assessment of plasma cortisol, levels of metabolites and the activity of several enzymes involved in carbohydrate metabolism. HSD conditions alone elicited important changes in energy metabolism of several tissues that in some cases were confirmatory (5-fold increase in plama cortisol, 20% increase in plasma glucose, 60% decrease in liver glycogen and 20% increase in gluconeogenic potential in the liver) whereas in others provided new information regarding metabolic adjustments to cope with HSD in the liver (100% increase in glucose phosphorylating capacity), gills (30% decrease in capacity for phosphorylating glucose), kidney (80% increase in the capacity of phosphorylating glucose) and brain (2.5-fold increase in ATP levels). On the other hand, food deprivation alone resulted in increased plasma cortisol, and metabolic changes in the liver (enhanced gluconeogenic and glycogenolytic potential of 13% and 18%, respectively) and brain (10% increase in glycolytic capacity), confirmatory of previous studies, whereas new information regarding metabolic adjustments during food deprivation was obtained in the gills and kidney (decreased lactate levels in both tissues of 45% and 55%, respectively). Furthermore, the results obtained provided, for the first time in fish, information indicating that food deprivation increased the sensitivity of gilthead sea bream to the stress induced by HSD compared with the fed controls, as demonstrated by increased plasma cortisol levels (50% increase vs

  6. In Situ Localization and Rhythmic Expression of Ghrelin and ghs-r1 Ghrelin Receptor in the Brain and Gastrointestinal Tract of Goldfish (Carassius auratus.

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    Aída Sánchez-Bretaño

    Full Text Available Ghrelin is a gut-brain peptide hormone, which binds to the growth hormone secretagogue receptor (GHS-R to regulate a wide variety of biological processes in fish. Despite these prominent physiological roles, no studies have reported the anatomical distribution of preproghrelin transcripts using in situ hybridization in a non-mammalian vertebrate, and its mapping within the different encephalic areas remains unknown. Similarly, no information is available on the possible 24-h variations in the expression of preproghrelin and its receptor in any vertebrate species. The first aim of this study was to investigate the anatomical distribution of ghrelin and GHS-R1a ghrelin receptor subtype in brain and gastrointestinal tract of goldfish (Carassius auratus using immunohistochemistry and in situ hybridization. Our second aim was to characterize possible daily variations of preproghrelin and ghs-r1 mRNA expression in central and peripheral tissues using real-time reverse transcription-quantitative PCR. Results show ghrelin expression and immunoreactivity in the gastrointestinal tract, with the most abundant signal observed in the mucosal epithelium. These are in agreement with previous findings on mucosal cells as the primary synthesizing site of ghrelin in goldfish. Ghrelin receptor was observed mainly in the hypothalamus with low expression in telencephalon, pineal and cerebellum, and in the same gastrointestinal areas as ghrelin. Daily rhythms in mRNA expression were found for preproghrelin and ghs-r1 in hypothalamus and pituitary with the acrophase occurring at nighttime. Preproghrelin, but not ghs-r1a, displayed a similar daily expression rhythm in the gastrointestinal tract with an amplitude 3-fold higher than the rest of tissues. Together, these results described for the first time in fish the mapping of preproghrelin and ghrelin receptor ghs-r1a in brain and gastrointestinal tract of goldfish, and provide the first evidence for a daily regulation

  7. Potential for bias in using hybrids between common carp (Cyprinus carpio) and goldfish (Carassius auratus) in endocrine studies: a first report of hybrids in Lake Mead, Nevada, U.S.A

    Science.gov (United States)

    Goodbred, Steven L.; Patino, Reynaldo; Orsak, Erik; Sharma, Prakash; Ruessler, Shane

    2013-01-01

    During a 2008 study to assess endocrine and reproductive health of common carp (Cyprinus carpio) in Lake Mead, Nevada (U.S.A.) we identified two fish, one male and one female, as hybrids with goldfish (Carassius auratus) based on morphology, lateral line scale count, and lack of anterior barbels. Gross examination of the female hybrid ovaries indicated presence of vitellogenic ovarian follicles; whereas histological evaluation of the male hybrid testes showed lobule-like structures with open lumens but without germ cells, suggesting it was sterile. Because common carp/goldfish hybrids are more susceptible to gonadal tumors and may have different endocrine profiles than common carp, researchers using common carp as a model for endocrine/reproductive studies should be aware of the possible presence of hybrids.

  8. Method for aquatic multiple species toxicant testing: acute toxicity of 10 chemicals to 5 vertebrates and 2 invertebrates. [Pimephales promelas; Carassius auratus; Ictalurus punctatus; Lepomis macrochirus; Salmo gairdneri; Orconectes immunis; Aplexa hypnorum

    Energy Technology Data Exchange (ETDEWEB)

    Phipps, G.L.; Holcombe, G.W.

    1985-01-01

    A method was developed to simultaneously ascertain 96 h LC/sub 50/ values for seven freshwater species in a single flow through test with measured concentrations. It allows interspecific comparisons, easy determination of the most sensitive species, and cuts cost of labor, materials and chemical analysis for measured concentration tests. Species tested included fathead minnows Pimephales promelas, goldfish Carassius auratus, channel catfish Ictalurus punctatus, bluegill Lepomis macrochirus, rainbow trout Salmo gairdneri, crayfish Orconectes immunis and snails Aplexa hypnorum. Compounds tested were pentachlorophenol, 2-chloroethanol, 2,4-pentanedione, hexachloroethane, ..cap alpha..-bromo-2',5'-dimethoxyacetophenone, benzaldehyde, 1,3-dichloro-4,6-dinitro-benzene, dursban, sevin and cadmium chloride. The LC/sub 50/ values from these multiple species tests compared favourably with those determined using single species tests at this laboratory, usually within 20%.

  9. Pilot-scale UV/H2O2 advanced oxidation process for municipal reuse water: Assessing micropollutant degradation and estrogenic impacts on goldfish (Carassius auratus L.).

    Science.gov (United States)

    Shu, Zengquan; Singh, Arvinder; Klamerth, Nikolaus; McPhedran, Kerry; Bolton, James R; Belosevic, Miodrag; Gamal El-Din, Mohamed

    2016-09-15

    Low concentrations (ng/L-μg/L) of emerging micropollutant contaminants in municipal wastewater treatment plant effluents affect the possibility to reuse these waters. Many of those micropollutants elicit endocrine disrupting effects in aquatic organisms resulting in an alteration of the endocrine system. A potential candidate for tertiary municipal wastewater treatment of these micropollutants is ultraviolet (UV)/hydrogen peroxide (H2O2) as an advanced oxidation process (AOP) which was currently applied to treat the secondary effluent of the Gold Bar Wastewater Treatment Plant (GBWWTP) in Edmonton, AB, Canada. A new approach is presented to predict the fluence-based degradation rate constants (kf') of environmentally occurring micropollutants including carbamazepine [(0.87-1.39) × 10(-3) cm(2)/mJ] and 2,4-Dichlorophenoxyacetic acid (2,4-D) [(0.60-0.91) × 10(-3) cm(2)/mJ for 2,4-D] in a medium pressure (MP) UV/H2O2 system based on a previous bench-scale investigation. Rather than using removal rates, this approach can be used to estimate the performance of the MP UV/H2O2 process for degrading trace contaminants of concern found in municipal wastewater. In addition to the ability to track contaminant removal/degradation, evaluation of the MP UV/H2O2 process was also accomplished by identifying critical ecotoxicological endpoints (i.e., estrogenicity) of the treated wastewater. Using quantitative PCR, mRNA levels of estrogen-responsive (ER) genes ERα1, ERα2, ERβ1, ERβ2 and NPR as well as two aromatase encoding genes (CYP19a and CYP19b) in goldfish (Carassius auratus L.) were measured during exposure to the GBWWTP effluent before and after MP UV/H2O2 treatment (a fluence of 1000 mJ/cm(2) and 20 mg/L of H2O2) in spring, summer and fall. Elevated expression of estrogen-responsive genes in goldfish exposed to UV/H2O2 treated effluent (a 7-day exposure) suggested that the UV/H2O2 process may induce acute estrogenic disruption to goldfish principally because

  10. Clinical Procedures Training for Veterinary Technicians and Investigators using Common Laboratory Animal Species, including: Mice (Mus musculus), Rats (Rattus norvegicus), Hamsters (Mesocricetus auratus), Guinea Pigs (Gavia porcellus), Rabbits (Otyctolagus cuniculus), Ferrets (Mustela putorius furo), Pigs (Sus scrofa), Sheep (Ovis aries), and Goats (Capra hircus)

    Science.gov (United States)

    2016-08-30

    60th Medical Group (AMC), Travis AFB, CA INSTITUTIONAL ANIMAL CARE AND USE COMMITTEE (IACUC) FINAL REPORT SUMMARY (Please !ml all information. Use...Technicians and Investigators using Common Laboratory Animal Species, including: Mice (Mus muscu/us), Rats (Rattus norvegicus), Hamsters (Mesocricetus auratus...DATE: 14 November 2016 FUNDING SOURCE: SG O&M funds LAST TRIENNIAL REVISION DATE: 15 October 2015 1. RECORD OF ANIMAL USAGE: Animal Species: Total

  11. Otolith morphometry and population parameters of red porgy, Pagrus pagrus (Linnaeus, 1758 in Saros Bay (North Aegean Sea.

    Directory of Open Access Journals (Sweden)

    Ali İşmen

    2015-12-01

    Full Text Available Bu çalışmada, Kuzey Ege Denizi’nde (Saroz Körfezi Fangri balığının toplam boy (TL ile toplam ağırlık (TW arasındaki ilişki, yaş, büyüme ve otolit boyu (OL - otolit ağırlığı (OW, balık boyu (TL - otolit boyu (OL, balık ağırlığı (TW - otolit boyu (OL, otolit boyu (OL - otolit genişliği (OWi arasındaki morfometrik ilişkiler incelenmiştir. Toplam 100 adet balık üzerinde yapılan ölçümlerde minimum boy 9,6 cm, maksimum boy 44,4 cm; minimum ağırlık 17,1 g, maksimum ağırlık 1372,5 g olarak tespit edilmiştir. Otolit ağırlığı minimum 0,0181 g, maksimum 0,1456 g; otolit boyu minimum 0,52 cm, maksimum 1,17 cm olarak ölçülmüştür. Boy-ağırlık ilişkisi TW=0,021*TL2,885 (r2=0,98, otolit boyu - otolit ağırlığı OW=0,105*OL2,609 (r2=0,92, toplam boy - otolit boyu OL=0,024*TL+0,249 (r2=0,83 otolit boyu - toplam ağırlık OL=0,235*TW0,237 (r2=0,81 otolit boyu - otolit genişliği OWi=0.501*OL+0.116 (r2=0.832 ilişkileri tespit edilmiştir. En büyük yaş 9 olarak belirlenmiş, von Bertalanffy büyüme parametreleri L∞=51,59 cm, K=0,12 yıl-1ve t0= -1,13 yıl olarak hesaplanmıştır

  12. Biología reproductiva del pargo Lutjanus inermis (Perciformes: Lutjanidae, en el Pacífico central mexicano Reproductive biology of the Golden snapper Lutjanus inermis (Perciformes: Lutjanidae in the central Mexican Pacific

    Directory of Open Access Journals (Sweden)

    Gabriela Lucano-Ramírez

    2012-03-01

    Full Text Available Debido a los escasos antecedentes del efecto de las pesquerías y manejo del pargo Lutjanus inermis, es importante conocer la época reproductiva para el establecimiento de temporadas de veda, ya que la biomasa de los peces en fase de reproducción, es usada como punto de referencia para evaluar la condición del stock explotado y para establecer futuros niveles de captura. Por ello, el objetivo de este trabajo fue analizar algunos aspectos reproductivos como proporción sexual, época reproductiva, talla de madurez de L. inermis en las costas de Jalisco, México. Durante 1999-2008, se llevaron a cabo muestreos mensuales de los ejemplares capturados por la pesca artesanal en las bahías de Navidad, Tenacatita y Chamela (Jalisco, México. Se recolectaron 311 (53.1% hembras y 275 (46.9% machos y, la proporción de sexos (1:0.88 no presentó diferencia significativa. El intervalo de longitud total fue 14.4 a 37.0cm y el peso total de 48 a 575g. L. inermis presentó los máximos valores del IGS en dos periodos, febrero-abril y septiembre-noviembre, también en estos periodos se presentaron los mayores porcentajes de organismos en estadio de maduración. El ovario presentó el tipo de desarrollo asincrónico y se reconocieron siete fases del desarrollo de los ovocitos. En los testículos se observó el tipo de desarrollo lobular, se observaron espermatozoides en el centro de los cistos y en el conducto recolector. La talla de madurez (L50 fue de 23.9cm LT para hembras y 23.6cm LT para machos. Considerando el periodo de muestreo de este estudio, los antecedentes descritos podrían ser de utilidad en la formulación de planes o estrategias de manejo de esta especie.Reproductive biology of the Golden snapper Lutjanus inermis (Perciformes: Lutjanidae in the central Mexican Pacific. Reproductive aspects and information on the effect of fishery activities over this species stock are scarce, despite the imperative need of effective protection and resource

  13. Parámetros hematológicos y bioquímicos en el hámster dorado (Mesocricetus auratus L. alimentado con base en harina de lombriz roja (Eisenia spp. y fuentes convencionales

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    L. Cova

    2011-01-01

    Full Text Available Se evaluó durante 21 días el efecto de cuatro dietas proteicas con base en harina de Lombriz Roja (Hl (Eisenia spp. (nivel de inclusión de Hl: D1: 21; D2: 24; D3: 27 y D4: 30% y dos alimentos convencionales: Conejarina ® (D0 y Ratarina® (D5 en algunos parámetros hematológicos y bioquímicos en el hámster dorado (Mesocrisetus auratus L. mediante un diseño totalmente aleatorizado para un factor, en el estado Trujillo, Venezuela. El hematocrito (37.65-41.01%, la hemoglobina (10.83-12.25%, la bilirrubina total, directa e indirecta (0.20-0.40; 0.10-0.22; 0.10-0.20mg/ dL, respectivamente, las lipoproteínas de muy baja densidad (Vldl (15.12-41.27mg/dL; los triglicéridos (95.42-190.54mg/dL; así como los valores de Calcio (Ca (9.83-12.32 mg/dL y Potasio (4.24-5.43mg/dL no exhibieron diferencias significativas entre dietas (P>0,05. Los leucocitos (Lto, las plaquetas (Plq, el conteo diferencial (neutrófilos/linfocitos (Linf/eosinófilos, la glicemia (Glc, la amilasa, el colesterol total, las lipoproteínas de elevada (Hdl y baja densidad y el Fósforo mostraron variaciones sustanciales entre los tratamientos (P<0,05. Los niveles crecientes de proteína de las dietas no afectaron los parámetros evaluados. Sin embargo, se observó una relación negativa entre la inclusión de Hl y las cantidades de Lto (r=-0.76**, Plq (r=-0.76**, Linf (r=-0.71**, Glc (r=-0.58* y Hdl (r=-0.50*. La adición de hl en proporciones de 24, 27 y 30% para obtener alimentos formulados entre 13.5 y 19.6% de proteína, para M. auratus, afecta algunos de los parámetros hematológicos y la concentración sérica de metabolitos relacionados con la composición nutricional de la dieta.

  14. Concentration of anesthetic for long-term exposure and the effects of inter-suture distance in the goldfish Carassius auratus - DOI: 10.4025/actascibiolsci.v31i2.802 Concentration of anesthetic for long-term exposure and the effects of inter-suture distance in the goldfish Carassius auratus - DOI: 10.4025/actascibiolsci.v31i2.802

    Directory of Open Access Journals (Sweden)

    Eduardo Shimoda

    2009-05-01

    Full Text Available This study aimed to determine the minimum concentration of 2-phenoxyethanol for long-term exposure and evaluate the effect of inter-suture distance on wound healing in the goldfish Carassius auratus. Twenty adult goldfish (standard length = 12.4 ± 1.1 cm; weight = 58.7 ± 17.2 g were anesthetized in 2-phenoxiethanol at 1.2‰ and placed in an anesthesia delivery system at the following concentrations of 2-phenoxiethanol: 0.0 (control; 0.1; 0.2; 0.3 and 0.4‰, and the duration of sedation was measured. Fifteen days later, fishes were anesthetized using the same procedure, and a 36 mm incision was performed in the ventro-lateral region. The incision was sutured using a simple-interrupted pattern with 3, 6 or 9 mm as inter-suture distances. Results demonstrated that 2-phenoxiethanol at 0.4‰ maintain the sedation for surgical procedures up to 60 minutes, and 9 mm as inter-suture distance optimized the wound healing in goldfish.This study aimed to determine the minimum concentration of 2-phenoxyethanol for long-term exposure and evaluate the effect of inter-suture distance on wound healing in the goldfish Carassius auratus. Twenty adult goldfish (standard length = 12.4 ± 1.1 cm; weight = 58.7 ± 17.2 g were anesthetized in 2-phenoxiethanol at 1.2‰ and placed in an anesthesia delivery system at the following concentrations of 2-phenoxiethanol: 0.0 (control; 0.1; 0.2; 0.3 and 0.4‰, and the duration of sedation was measured. Fifteen days later, fishes were anesthetized using the same procedure, and a 36 mm incision was performed in the ventro-lateral region. The incision was sutured using a simple-interrupted pattern with 3, 6 or 9 mm as inter-suture distances. Results demonstrated that 2-phenoxiethanol at 0.4‰ maintain the sedation for surgical procedures up to 60 minutes, and 9 mm as inter-suture distance optimized the wound healing in goldfish.

  15. PERBEDAAN TINGKAT STRES LUTUNG JAWA (Trachypitecus auratus PADA KANDANG PERAWATAN DAN KANDANG KARANTINA DI JAVAN LANGUR CENTER (JLC DITINJAU DARI KADAR KORTISOL DAN RASIO NEUTROFIL PERLIMFOSIT (N/L

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    Eko Dini Danafi

    2017-11-01

    Full Text Available Lutung Jawa (Trachypithecus auratus adalah satwa endemik Jawa dan Bali. Populasi lutung Jawa semakin mengalami penurunan dan masuk kategori satwa yang dilindungi. Penurunan populasi lutung jawa dapat disebabkan oleh berbagai macam penyakit yang diperparah dengan tingginya tingkat stres pada satwa. Javan Langur Center yang berlokasi di Cangar, Batu merupakan salah satu tempat untuk merehabilitasi lutung Jawa sebelum dilepasliarkan ke alam. Javan Langur Center terdiri dari tiga kandang yaitu kandang perawatan, kandang karantina dan kandang sosialisasi. Respon stress pada primata dapat dievaluasi dengan menggunakan indikator pengukuran hormon kortisol dan rasio neutrofil per limfosit. Penelitian bertujuan untuk mengetahui perbedaan kadar kortisol dan rasio neutrofil per limfosit sebagai indikator stres pada lutung Jawa di kandang perawatan dengan kandang karantina. Lutung Jawa dengan umur 2-7,5 tahun dan berjenis kelamin jantan dan betina. Pengukuran kadar kortisol dengan metode ELISA Indirect gelombang pada 450 nm dan diferensial leukosit diukur dengan hematology analizer. Hasil penelitian menunjukkan bahwa sampel pada kandang perawatan memiliki rata-rata kadar kortisol yang lebih tinggi (116,1 ng/mL daripada kandang karantina (87,5 ng/mL, dan pengukuran rasio N/L pada kandang perawatan lebih tinggi (11,3 daripada kandang karantina (1,2.

  16. Japon Balığı (Carassius Auratus L. 1758) ve Arpa Bitkisinin (Hordeum Vulgare L.) Gelişimi ve Su Kalitesinin İyileştirilmesi Üzerine Aquaponik Sistemin Etkileri

    OpenAIRE

    KESKİNBALTA, Mehmet Anıl; HAMZAOĞLU, Gökhan; ÇELİK, Meryem Yeşim; DERNEKBAŞI, Seval

    2015-01-01

    Bu araştırmada, Japon balığı (Carassius auratus L. 1758) ve arpa bitkisi (Hordeum vulgare L.) kullanılarak model bir akuaponik sistem oluşturulmuştur. Araştırma süresince arpa bitkisinin suyun nitrit, nitrat ve fosfat değerlerinde yaptığı değişim ve balıkların gelişimi üzerindeki etkilerinin belirlenmesi amaçlanmıştır. 30 günlük araştırma süresince günlük olarak pH, sıcaklık ve oksijen değerleri ölçülmüştür. Haftalık olarak bitki yetiştirme yatağına giren ve bitkiden süzülen suyun nitrit (NO2...

  17. Japon Balığı (Carassius auratus L. 1758) ve Arpa Bitkisinin (Hordeum vulgare L.) Gelişimi ve Su Kalitesinin İyileştirilmesi Üzerine Aquaponik Sistemin Etkileri

    OpenAIRE

    KESKİNBALTA, Mehmet; HAMZAOĞLU, Gökhan; ÇELİK, Meryem; DERNEKBAŞI, Seval

    2015-01-01

    Bu araştırmada, Japon balığı (Carassius auratus L. 1758) ve arpa bitkisi (Hordeum vulgare L.) kullanılarak model bir akuaponik sistem oluşturulmuştur. Araştırma süresince arpa bitkisinin suyun nitrit, nitrat ve fosfat değerlerinde yaptığı değişim ve balıkların gelişimi üzerindeki etkilerinin belirlenmesi amaçlanmıştır. 30 günlük araştırma süresince günlük olarak pH, sıcaklık ve oksijen değerleri ölçülmüştür. Haftalık olarak bitki yetiştirme yatağına giren ve bitkiden süzülen suyun nitrit (NO2...

  18. Dactylogyrids (Monogenoidea: Polyonchoinea parasitizing the gills of snappers (Perciformes: Lutjanidae: revision of Euryhaliotrema with new and previously described species from the Red Sea, Persian Gulf, the eastern and Indo-west Pacific Ocean, and the Gulf of Mexico

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    Delane C. Kritsky

    2012-06-01

    Full Text Available Twenty one of 29 species of snappers (Lutjanidae, examined for dactylogyrids (Monogenoidea from the Red Sea, Persian Gulf, the Indo-west and eastern Pacific Ocean, the Gulf of Mexico, and Caribbean Sea were parasitized by 16 new and 11 previously described species of Euryhaliotrema: Euryhaliotrema adelpha sp. nov., Euryhaliotrema anecorhizion sp. nov., Euryhaliotrema cardinale sp. nov., Euryhaliotrema chrysotaeniae, Euryhaliotrema cognatus sp. nov., Euryhaliotrema cryptophallus sp. nov., Euryhaliotrema diplops sp. nov., Euryhaliotrema distinctum sp. nov., Euryhaliotrema fajeravilae sp. nov., Euryhaliotrema fastigatum, Euryhaliotrema fatuum sp. nov., Euryhaliotrema ferocis sp. nov., Euryhaliotrema hainanense, Euryhaliotrema longibaculum, Euryhaliotrema mehen comb. nov., Euryhaliotrema paracanthi, Euryhaliotrema paululum sp. nov., Euryhaliotrema perezponcei, Euryhaliotrema ramulum sp. nov., Euryhaliotrema seyi sp. nov., Euryhaliotrema simplicis sp. nov., Euryhaliotrema spirotubiforum, Euryhaliotrema tormocleithrum sp. nov., Euryhaliotrema torquecirrus, Euryhaliotrema tubocirrus, Euryhaliotrema xinyingense, and Euryhaliotrema youngi sp. nov. Six species of Euryhaliotrema, previously reported from lutjanid hosts, were not collected: Euryhaliotrema anguiformis comb. nov., Euryhaliotrema guangdongense, Euryhaliotrema johni, Euryhaliotrema lutiani, Euryhaliotrema lutjani, and Euryhaliotrema nanaoense comb. nov. The diagnosis of Euryhaliotrema was emended to include species having tandem or slightly overlapping gonads, a pretesticular germarium, a globose haptor with morphologically similar anchors and hooks, a coiled or meandering male copulatory organ, a dextral vaginal pore, and hooks with upright acute thumbs and slender shanks comprised of one subunit. A bulbous base of the MCO and presence of an accessory piece in the copulatory complex were no longer considered features defining the genus. As a result, Euryhaliotrematoides and Aliatrema were

  19. Do nursery habitats provide shelter from flow for juvenile fish?

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    Darren M Parsons

    Full Text Available Juvenile fish nurseries are an essential life stage requirement for the maintenance of many fish populations. With many inshore habitats globally in decline, optimising habitat management by increasing our understanding of the relationship between juvenile fish and nursery habitats may be a prudent approach. Previous research on post-settlement snapper (Chrysophrys auratus has suggested that structure may provide a water flow refuge, allowing snapper to access high water flow sites that will also have a high flux of their pelagic prey. We investigated this hypothesis by describing how Artificial Seagrass Units (ASUs modified water flow while also using a multi-camera set up to quantify snapper position in relation to this water flow environment. Horizontal water flow was reduced on the down-current side of ASUs, but only at the height of the seagrass canopy. While the highest abundance of snapper did occur down-current of the ASUs, many snapper also occupied other locations or were too high in the water column to receive any refuge from water flow. The proportion of snapper within the water column was potentially driven by strategy to access zooplankton prey, being higher on the up-current side of ASUs and on flood tides. It is possible that post-settlement snapper alternate position to provide opportunities for both feeding and flow refuging. An alternative explanation relates to an observed interaction between post-settlement snapper and a predator, which demonstrated that snapper can utilise habitat structure when threatened. The nature of this relationship, and its overall importance in determining the value of nursery habitats to post-settlement snapper remains an elusive next step.

  20. Do nursery habitats provide shelter from flow for juvenile fish?

    Science.gov (United States)

    Parsons, Darren M; MacDonald, Iain; Buckthought, Dane; Middleton, Crispin

    2018-01-01

    Juvenile fish nurseries are an essential life stage requirement for the maintenance of many fish populations. With many inshore habitats globally in decline, optimising habitat management by increasing our understanding of the relationship between juvenile fish and nursery habitats may be a prudent approach. Previous research on post-settlement snapper (Chrysophrys auratus) has suggested that structure may provide a water flow refuge, allowing snapper to access high water flow sites that will also have a high flux of their pelagic prey. We investigated this hypothesis by describing how Artificial Seagrass Units (ASUs) modified water flow while also using a multi-camera set up to quantify snapper position in relation to this water flow environment. Horizontal water flow was reduced on the down-current side of ASUs, but only at the height of the seagrass canopy. While the highest abundance of snapper did occur down-current of the ASUs, many snapper also occupied other locations or were too high in the water column to receive any refuge from water flow. The proportion of snapper within the water column was potentially driven by strategy to access zooplankton prey, being higher on the up-current side of ASUs and on flood tides. It is possible that post-settlement snapper alternate position to provide opportunities for both feeding and flow refuging. An alternative explanation relates to an observed interaction between post-settlement snapper and a predator, which demonstrated that snapper can utilise habitat structure when threatened. The nature of this relationship, and its overall importance in determining the value of nursery habitats to post-settlement snapper remains an elusive next step.

  1. Ghrelin suppresses cholecystokinin (CCK), peptide YY (PYY) and glucagon-like peptide-1 (GLP-1) in the intestine, and attenuates the anorectic effects of CCK, PYY and GLP-1 in goldfish (Carassius auratus).

    Science.gov (United States)

    Blanco, Ayelén Melisa; Bertucci, Juan Ignacio; Valenciano, Ana Isabel; Delgado, María Jesús; Unniappan, Suraj

    2017-07-01

    Ghrelin is an important gut-derived hormone with an appetite stimulatory role, while most of the intestinal hormones, including cholecystokinin (CCK), peptide YY (PYY) and glucagon-like peptide-1 (GLP-1), are appetite-inhibitors. Whether these important peptides with opposing roles on food intake interact to regulate energy balance in fish is currently unknown. The aim of this study was to characterize the putative crosstalk between ghrelin and CCK, PYY and GLP-1 in goldfish (Carassius auratus). We first determined the localization of CCK, PYY and GLP-1 in relation to ghrelin and its main receptor GHS-R1a (growth hormone secretagogue 1a) in the goldfish intestine by immunohistochemistry. Colocalization of ghrelin/GHS-R1a and CCK/PYY/GLP-1 was found primarily in the luminal border of the intestinal mucosa. In an intestinal explant culture, a significant decrease in prepro-cck, prepro-pyy and proglucagon transcript levels was observed after 60min of incubation with ghrelin, which was abolished by preincubation with the GHS-R1a ghrelin receptor antagonist [D-Lys3]-GHRP-6 (except for proglucagon). The protein expression of PYY and GLP-1 was also downregulated by ghrelin. Finally, intraperitoneal co-administration of CCK, PYY or GLP-1 with ghrelin results in no modification of food intake in goldfish. Overall, results of the present study show for the first time in fish that ghrelin exerts repressive effects on enteric anorexigens. It is likely that these interactions mediate the stimulatory effects of ghrelin on feeding and metabolism in fish. Copyright © 2017 Elsevier Inc. All rights reserved.

  2. Light and scanning electron microscopic studies of Myxobolus indica n. sp. and a report of three Myxozoan (Myxosporea: Bivalvulida parasites of cultured ornamental goldfish, Carassius auratus L. for the first time in India

    Directory of Open Access Journals (Sweden)

    Mandira Saha

    2017-08-01

    Full Text Available The ornamental fish industry is an economically viable sector in India which suffers from different ectoparasitic infestations, including the myxozoan parasites. An icthyoparasitological survey of myxozoan infections in ornamental fish farms in India revealed the presence of four myxozoan parasites belonging to the family Myxobolidae, in the genera Myxobolus and Thelohanellus. The myxozoan spores were small to large, spherical to ellipsoidal in size. The plasmodia measured 0.5–3.0 mm in diameter with disporic pansporoblasts and mature spores. During the survey the authors identified for the first time in India, three previously described species, namely, M. mehlhorni, T. nikolskii and T. batae; and one new species M. indica n. sp., all infecting the ornamental goldfish, Carassius auratus. The present study thus reports a new host, and a new locality for T. batae and M. mehlhorni. The description of T. nikolskii is the first record found in India. The spore of M. indica n. sp. measures 5.8 ± 0.2 × 4.1 ± 0.5 μm in size, having two equal shaped pyriform polar capsules measuring 4.1 ± 0.4 × 2.7 ± 0.6 μm. The results from a combination of light and scanning electron microscopic observations along with a comparison with closely related species were incorporated here. Molecular data is needed to complete the description of the new species.

  3. Monocrotophos pesticide decreases the plasma levels of total 3,3',5-triiodo-l-thyronine and alters the expression of genes associated with the thyroidal axis in female goldfish (Carassius auratus.

    Directory of Open Access Journals (Sweden)

    Xiaona Zhang

    Full Text Available Our recent study showed that monocrotophos (MCP pesticide disrupted the hypothalamic-pituitary-thyroid (HPT axis in male goldfish (Carassius auratus; however, the effects of MCP on the thyroid system in female goldfish are remain unclear. In the present study, plasma thyroid hormone (TH and thyroid-stimulating hormone (TSH levels were evaluated in female goldfish exposed to 0.01, 0.10, and 1.00 mg/L of 40% MCP-based pesticide for 21 days in a semi-static exposure system. Expression profiles of HPT axis-responsive genes, including transthyretin (ttr, deiodinases (d1, d2, and d3, tshβ, thyrotropin-releasing hormone (trh, and corticotrophin-releasing hormone (crh, were determined. The results indicated that MCP decreased the plasma levels of total 3,3',5-triiodo-l-thyronine (TT3 and the ratio of TT3 to total 3,3',5,5'-l-thyroxine (TT4, and induced alternative expression of TH-related genes. Exposure to 0.01 and 0.10 mg/L MCP pesticide resulted in the up-regulation of ttr mRNA. The reduction of plasma TT3 levels was partly attributed to an increase in the metabolism of T3 in the liver, as revealed by the highly elevated hepatic d1 and d3 mRNA levels in the MCP treatment groups, and the expression of hepatic d3 showed a negative correlation with the plasma TT3/TT4 levels in females. Moreover, the plasma TSH levels were lower in females exposed to 0.01 and 0.10 mg/L MCP pesticide, whereas the up-regulation of tshβ mRNA levels was compensated by the decreased plasma TT3 levels. These results indicated that MCP had the potential to influence several pathways of HPT axis homeostasis in female goldfish.

  4. La inmunocromatografía para el diagnóstico de la infección por Taenia solium en Mesocricetus auratus mediante la detección de coproantígenos*

    Directory of Open Access Journals (Sweden)

    Hermes Escalante A

    2001-07-01

    Full Text Available Objetivo: Evaluar la técnica de inmunocromatografía para el diagnóstico de la infección por Taenia solium en Mesocricetus auratus "hámsters" mediante la detección de coproantígenos. Materiales y métodos: Se produjo en Oryctolagus cunniculus "conejo" IgG anti-antígenos de excreción/secreción (ES de T. solium y en Capra hircus "cabra" IgG anti-IgG de conejo. Parte de las IgG anti-antígenos ES de T. solium fue conjugado con oro coloidal. La inmunocromatografía se llevó a cabo utilizando tiras de nitrocelulosa en las cuáles se fijó dos bandas con anticuerpos de captura; en la primera, las IgG anti-antígenos ES de T. solium (banda de prueba, y en la segunda las IgG anti-IgG de conejo (banda control. La técnica fue evaluada con un "pool" de tres muestras de medio de cultivo con antígenos ES de T. solium, Hymenolepis nana, Dyphillobotrium pacificum y de larvas de Anisakis simplex, así como con un "pool" de tres muestras fecales de hámsters no parasitados, de parasitados por H. nana y de siete muestras fecales de hámsters parasitados por T. Solium. Resultados: La inmunocromatografía permitió detectar antígenos de T. solium en muestras de medio de cultivo con antígenos ES específicas y en heces de hámsters parasitados por T. solium mediante la coloración de la banda de prueba, siendo negativo cuando se utilizó medios con ES de otros helmintos y sin antígenos, así como con muestras de heces de hámsters infectados por otros parásitos y de no parasitados. En todas las tiras usadas se observó coloración de la banda control. Conclusión: La técnica de inmunocromatografía es capaz de detectar antígenos de T. solium, tanto en medio de cultivo como en heces de hámsters, faltando evaluar la sensibilidad y la especificidad con muestras fecales humanas para el diagnóstico de la teniosis mediante la detección de coproantígenos.

  5. Spatial and dietary overlap between red snapper and vermilion snapper (NCEI Accession 0130920)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains abundance data for nine species of reef fish observed at 40 reef sites between Mobile Bay, Alabama and Saint Andrews Bay, Florida, during...

  6. Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major).

    Science.gov (United States)

    Sawayama, Eitaro; Tanizawa, Shiho; Kitamura, Shin-Ichi; Nakayama, Kei; Ohta, Kohei; Ozaki, Akiyuki; Takagi, Motohiro

    2017-12-01

    Red sea bream iridoviral disease (RSIVD) is a major viral disease in red sea bream farming in Japan. Previously, we identified one candidate male individual of red sea bream that was significantly associated with convalescent individuals after RSIVD. The purpose of this study is to identify the quantitative trait loci (QTL) linked to the RSIVD-resistant trait for future marker-assisted selection (MAS). Two test families were developed using the candidate male in 2014 (Fam-2014) and 2015 (Fam-2015). These test families were challenged with RSIV, and phenotypes were evaluated. Then, de novo genome sequences of red sea bream were obtained through next-generation sequencing, and microsatellite markers were searched and selected for linkage map construction. One immune-related gene, MHC class IIβ, was also used for linkage map construction. Of the microsatellite markers searched, 148 and 197 were mapped on 23 and 27 linkage groups in the female and male linkage maps, respectively, covering approximately 65% of genomes in both sexes. One QTL linked to an RSIVD-resistant trait was found in linkage group 2 of the candidate male in Fam-2014, and the phenotypic variance of the QTL was 31.1%. The QTL was closely linked to MHC class IIβ. Moreover, the QTL observed in Fam-2014 was also significantly linked to an RSIVD-resistant trait in the candidate male of Fam-2015. Our results suggest that the RSIVD-resistant trait in the candidate male was controlled by one major QTL closely linked to the MHC class IIβ gene and could be useful for MAS of red sea bream.

  7. PIXE analysis of otoliths from reared red sea bream, pagrus major (temminck et schlegl)

    International Nuclear Information System (INIS)

    Arai, Nobuaki; Sakamoto, Wataru

    1993-01-01

    PIXE analysis was applied to estimate mineral concentration in red sea bream otoliths without cutting or polishing. Detected elements include Sr, Fe, Mn and Zn, which are commonly found in the otoliths in marine fishes. Strontium-Calcium concentration ratio is calculated by means of combined X-ray yields. The ratio doesn't indicate clear correlaion with mean reared seawater temperature. It is caused by the diffraction error induced by rough topographies of the otoliths surface. (author)

  8. Spatial Distribution of Reef Fish Species along the Southeast US Atlantic Coast Inferred from Underwater Video Survey Data.

    Directory of Open Access Journals (Sweden)

    Nathan M Bacheler

    Full Text Available Marine fish abundance and distribution often varies across spatial scales for a variety of reasons, and this variability has significant ecological and management consequences. We quantified the distribution of reef-associated fish species along the southeast United States Atlantic coast using underwater video survey samples (N = 4,855 in 2011-2014 to elucidate variability within species across space, depths, and habitats, as well as describe broad-scale patterns in species richness. Thirty-two species were seen at least 10 times on video, and the most commonly observed species were red porgy (Pagrus pagrus; 41.4% of videos, gray triggerfish (Balistes capriscus; 31.0%, black sea bass (Centropristis striata; 29.1%, vermilion snapper (Rhomboplites aurorubens; 27.7%, and red snapper (Lutjanus campechanus; 22.6%. Using generalized additive models, we found that most species were non-randomly distributed across space, depths, and habitats. Most rare species were observed along the continental shelf break, except for goliath grouper (Epinephelus itajara, which was found on the continental shelf in Florida and Georgia. We also observed higher numbers of species in shelf-break habitats from southern North Carolina to Georgia, and fewer in shallower water and at the northern and southern ends of the southeast United States Atlantic coast. Our study provides the first broad-scale description of the spatial distribution of reef fish in the region to be based on fishery-independent data, reinforces the utility of underwater video to survey reef fish, and can help improve the management of reef fish in the SEUS, for example, by improving indices of abundance.

  9. 额尔齐斯河2种类型雌性银鲫的形态特征及D-loop基因序列比较%Comparative analysis between two types of female silver crucian carp Carassius auratus gibelio in the Irtysh River using morphology and mitochondrial control regions

    Institute of Scientific and Technical Information of China (English)

    马波; 霍堂斌; 李喆; 蔡林钢; 阿达可白克可尔江; 姜作发

    2013-01-01

    Two types of female silver crucian carp Carassius auratus gibelio were discovered in the main stem of the Irtysh Rive (the Crop 185) in May 2008. We defined them as high-dorsal silver crucian carp (1.78−2.14) and low-dorsal silver crucian carp (2.18−2.39) based on differences in their morphology (body length/body depth). The number of lateral line scales in the high and low-dorsal silver crucian carp is 31 and 30, respectively. The number of scales above (or below) the lateral line is 7 (6), the number of scales above (or below) the lateral line in the caudal peduncle is 3 (2). High-dorsal silver crucian carp exhibit a size advantage based on the significant differ-ence in body depth and width, head depth and width, and caudal peduncle depth (P<0.01) between high and low-dorsal silver crucian carp. The mean weight of high-dorsal silver crucian carp is 1.3−1.9 times that of low-dorsal silver crucian carp. A comparison of the mitochondrial control region sequences of the two types re-vealed that each has one haplotype, thus representing intraspecific variation with a low genetic diversity value (0.004). The two types and Fangzheng silver crucian carp in the Heilongjiang River are monophylic, and share the same haplotype with Fangzheng silver crucian carp. Accordingly, we speculate that the Irtysh silver crucian carps are derived from the Heilongjiang River basin. Our observations suggest the two types of C. a. gibelio, particularly the high-dorsal silver crucian carp, have a growth advantage over other crucian carps. Given this, they offer greater potential as breeding stock. Our results provide a theoretical basis for better conservation and utilization of the unique varieties of Irtysh River silver crucian carp.%  2008年5月调查发现,在额尔齐斯河主河道(185团)的雌性银鲫(Carassius auratus gibelio)群体中存在2种形态类型,按照体长比体高的形态学性状区分,分别为高背型(1.78~2.14)和低背型(2.18~2.39).形态学分

  10. 78 FR 17336 - Snapper-Grouper Fishery off the Southern Atlantic States; Snapper-Grouper Management Measures

    Science.gov (United States)

    2013-03-21

    ... nation, particularly with respect to providing food production and recreational opportunities, and... and sustainability of the fishery and how many fish can be harvested the following year. Since the... identified. The rule would apply directly to licensed commercial fishermen in the Finfish Fishing Industry...

  11. 77 FR 42688 - Snapper-Grouper Fishery Off the Southern Atlantic States; Snapper-Grouper Management Measures

    Science.gov (United States)

    2012-07-20

    ... values as a catch limit is a risk-averse approach that sacrifices some yield over the short-term to gain... Islands. Dated: July 17, 2012. Samuel D. Rauch III, Deputy Assistant Administrator for Regulatory Programs...

  12. 77 FR 61295 - Snapper-Grouper Fishery off the Southern Atlantic States; Snapper-Grouper Management Measures

    Science.gov (United States)

    2012-10-09

    ... recreational sector. The third alternative would specify a recreational sector in-season AM and includes two... tilefish commercial sector with a commercial trip limit of 300 lb (136 kg) for the 2012 fishing year. The... recreational sectors in the South Atlantic based on new stock assessment analyses. DATES: This rule is...

  13. Effects of Polybrominated Diphenyl Ethers on DNA Damage and p53 Protein Expression in Carassius auratus Linn.%多溴联苯醚对鲫鱼组织DNA损伤及p53蛋白表达的影响

    Institute of Scientific and Technical Information of China (English)

    吴伟; 瞿建宏; 陈家长; 聂凤琴

    2011-01-01

    以鲫鱼(Carawsius auratus)为试验材料,研究了经不同浓度的2,2’,4,4’-四溴联苯醚(PBDE-47)和十溴联苯醚(PBDE-209)暴露后,鲫鱼体内DNA损伤产物8-羟基脱氧鸟苷(8-OHdG)的含量及p53蛋白的水平.结果表明,采用浓度为0.10~5.00 mg·L-1的PBDE-47和10.0~50.0mg·L-1的PBDE-209处理鲫鱼20d,除了0.10 mg·L-1 PBDE-47和10.0 mg·L-1 PBDE-209浓度组的8-OHdG和p53蛋白含量与对照组相比无显著差异(P>0.05),其余各浓度组的8-OHdG和p53蛋白含量均随PBDE-47和PBDE-209浓度增加而逐渐上升,呈显著的相关关系(P<0.01),且8-OHdG和p53蛋白之间也呈显著的正相关.说明PBDE-47和PBDE-209对鲫鱼组织的DNA产生了损伤,具有遗传毒性影响.%Persistent organic pollutants(POPs) has a long-term threat to different biota, including aquatic animals, mammals, especially humans, due to their biomagnified effect in food chain, the study on the toxic effect of POPs to aquatic animals was evidently important and concerned. This paper discussed the variation of DNA-damage product 8-Hydroxy-desoxyguanosine(8-OHdG) and p53 protein to Carassius awatus in exposed to poly -brominated diphenyl ethers (PBDE-47 and PBDE-209), where the exposed dose of PBDE-47 and PBDE-209 ranged from 0.10 to 5.00 mg·L-1 and 10.0 to 50.0 mg·L-1, respectively. The results showed that, DNA-damage product 8-OHdC and p53 protein were generated extraordinary in all treatment lasted for 20 days, expectihe 0.1 mg·L-1 in PBDE-47 and 10.0 mg·L-1 in PBDE-209. The variation of 8-OHdC and p53 protein to Carassius awatus when exposed to PBDE-47 and PBDE-209 were significantly dose-depend (P<0.01), respectively, and the variation of 8-OHdC was significantly correlated with the variation of p53 protein as well. The results indicated that, the genotoxic effect, characterized by DNA-damage product, such as 8-OHdC and p53 protein in Carassius awatus when exposed to PBDE-47 and PBDE-209 were produced. The paper provided more

  14. Sensibility of the hamster (Cricetus auratus to the Treponema pertenue

    Directory of Open Access Journals (Sweden)

    F. Nery-Guimarães

    1955-05-01

    Full Text Available In two experiments, 8 Hamsters inoculated with material from yaws lesions (Treponema pertenue, developed skin lesions considered specific by their clinical and histopathological aspects and by the presence of treponemae. These lesions appeared on the scrotumm, testicle, prepuce, anus, tail, muzzle, back and hinders paws (palm surface. In the internal organs no treponemae were found in direct examinations and inoculation of brain, spleen and lymph node. The incubation period was of 35 days for the testicle, 55 days for the scrotum and 107 days for peritoneal cavity inoculation. Positive sub-inoculations were obtained. The serum reactions (Qasserman's and Kahn's were negative in all 5 tested Hamsters. Out of 4 normal females matched to infected males two developed nasal lesions resulting from direct contact. Apparently the genital lesions hindered copulation. Hamsters are very well suited for an experimental study of yaws.Em 2 experiências, 8 Hamsters inoculados com material direto de lesões boubáticas (Treponema pertenue, desenvolveram lesões cutâneas consideradas específicas, pelo aspecto clínico e histopatológico e pela presentça de treponemas. Essas lesões se manifestaram no escrôto, testículo, prepúcio, anus, cauda, focinho, dorso e patas posteriores (face palmar. Nos órgãos internos não foram vistos treponemas ao exame direto e, uma vez, por inoculação de cérebro, baço e gânglio linfático. O período incubativo foi de 35 dias pela via testicular, 55 dias pela via escrotal e 107 dias pela via peritonial. Foram obtidas sub-inoculações positivas para Hamsters normais. As experiências continuam. De 4 fêmeas normais, acasaladas com 4 hamsters infectados apenas 2 mostraram lesões positivas resultantes de contágio direto. Aparentemente, não houve copulação e, se esta ocorreu, não determinou fecundação.

  15. Enhanced longevity in tau mutant Syrian hamsters, Mesocricetus auratus

    NARCIS (Netherlands)

    Oklejewicz, Malgorzata; Daan, Serge

    The single-gene mutation tau in the Syrian hamster shortens the circadian period by about 20% in the homozygous mutant and simultaneously increases the mass-specific metabolic rate by about 20%. Both effects might be expected to lead to a change in longevity. To test such expectations, the life span

  16. Morphology of the intestine of the Goldfish (Carassius auratus)

    NARCIS (Netherlands)

    Weinberg, Steven

    1976-01-01

    A survey of the structures of the intestine of the Goldfish is given. Several morphological techniques have been used. Special attention has been paid to those characteristics of this organ that may be of importance to physiologists working with the same material, with emphasis on cell-to-cell

  17. Starvation beneficially influences the liver physiology and nutrient metabolism in Edwardsiella tarda infected red sea bream (Pagrus major).

    Science.gov (United States)

    Mohapatra, Sipra; Chakraborty, Tapas; Shimizu, Sonoko; Urasaki, Shintaro; Matsubara, Takahiro; Nagahama, Yoshitaka; Ohta, Kohei

    2015-11-01

    Dietary compromises, especially food restrictions, possess species-specific effects on the health status and infection control in several organisms, including fish. To understand the starvation-mediated physiological responses in Edwardsiella tarda infected red sea bream, especially in the liver, we performed a 20-day starvation experiment using 4 treatment (2 fed and 2 starved) groups, namely, fed-placebo, starved-placebo, fed-infected, and starved-infected, wherein bacterial exposure was done on the 11th day. In the present study, the starved groups showed reduced hepatosomatic index and drastic depletion in glycogen storage and vacuole formation. The fed-infected fish showed significant (Pred sea bream. Copyright © 2015 Elsevier Inc. All rights reserved.

  18. Effect of the squid viscera hydrolysate on growth performance and digestion in the red sea bream Pagrus major.

    Science.gov (United States)

    Kondo, Fumitaka; Ohta, Takashi; Iwai, Toshiharu; Ido, Atsushi; Miura, Chiemi; Miura, Takeshi

    2017-12-01

    The improvement in feed efficiency is one of the most important subjects in fish culture. The development of feed, in terms of good intake, high growth performance, and high feed efficiency is needed. Squid viscera are one of the candidates for alternative material in improving feed efficiency in fish culture. In the present study, we described the dietary effect of the squid viscera hydrolysate (SVH) on the growth performance of the red sea bream. The addition of SVH to feed caused significant increases in feed intake, fork length, and body weight and produced a marked improvement in feed conversion after 4 weeks of feeding. Furthermore, the results of this feeding revealed that low dietary levels of SVH promote growth performance in the red sea bream. We physiologically analyzed digestion and appetite in fish fed diet containing SVH. SVH promoted the activity of hepatic trypsin and lipase, gene expression of stomach pepsin, hepatic lipase, and pyloric caeca trypsin, thereby improving the nutrient availability in red sea bream. Moreover, the mRNA expression of appetite regulating factor, such as brain NPY and stomach ghrelin was significantly improved by dietary SVH. Our current results indicate that dietary SVH as alternative material produced excellent effects on growth performance, which is dependent on the promoting effect on digestion and appetite in red sea bream.

  19. Identification, Characterization, and Mapping of a Novel SNP Associated with Body Color Transparency in Juvenile Red Sea Bream (Pagrus major).

    Science.gov (United States)

    Sawayama, Eitaro; Noguchi, Daiki; Nakayama, Kei; Takagi, Motohiro

    2018-03-23

    We previously reported a body color deformity in juvenile red sea bream, which shows transparency in the juvenile stage because of delayed chromatophore development compared with normal individuals, and this finding suggested a genetic cause based on parentage assessments. To conduct marker-assisted selection to eliminate broodstock inheriting the causative gene, developing DNA markers associated with the phenotype was needed. We first conducted SNP mining based on AFLP analysis using bulked-DNA from normal and transparent individuals. One SNP was identified from a transparent-specific AFLP fragment, which significantly associated with transparent individuals. Two alleles (A/G) were observed in this locus, and the genotype G/G was dominantly observed in the transparent groups (97.1%) collected from several production lots produced from different broodstock populations. A few normal individuals inherited the G/G genotype (5.0%), but the A/A and A/G genotypes were dominantly observed in the normal groups. The homologs region of the SNP was searched using a medaka genome database, and intron 12 of the Nell2a gene (located on chromosome 6 of the medaka genome) was highly matched. We also mapped the red sea bream Nell2a gene on the previously developed linkage maps, and this gene was mapped on a male linkage group, LG4-M. The newly found SNP was useful in eliminating broodstock possessing the causative gene of the body color transparency observed in juvenile stage of red sea bream.

  20. Connectivity of a large embayment and coastal fishery: spawning aggregations in one bay source local and broad-scale fishery replenishment.

    Science.gov (United States)

    Hamer, P A; Acevedo, S; Jenkins, G P; Newman, A

    2011-04-01

    Ichthyoplankton sampling and otolith chemistry were used to determine the importance of transient spawning aggregations of snapper Chrysophrys auratus (Sparidae) in a large embayment, Port Phillip Bay (PPB), Australia, as a source of local and broad-scale fishery replenishment. Ichthyoplankton sampling across five spawning seasons within PPB, across the narrow entrance to the bay and in adjacent coastal waters, indicated that although spawning may occur in coastal waters, the spawning aggregations within the bay were the primary source of larval recruitment to the bay. Otolith chemical signatures previously characterized for 0+ year C. auratus of two cohorts (2000 and 2001) were used as the baseline signatures to quantify the contribution that fish derived from reproduction in PPB make to fishery replenishment. Sampling of these cohorts over a 5 year period at various widely dispersed fishery regions, combined with maximum likelihood analyses of the chemistry of the 0+ year otolith portions of these older fish, indicated that C. auratus of 1 to 3+ years of age displayed both local residency and broad-scale emigration from PPB to populate coastal waters and an adjacent bay (Western Port). While the PPB fishery was consistently dominated (>70%) by locally derived fish irrespective of cohort or age, the contribution of fish that had originated from PPB to distant populations increased with age. At 4 to 5+ years of age, when C. auratus mature and fully recruit to the fishery, populations of both cohorts across the entire central and western Victorian fishery, including two major embayments and c. 800 km of coastal waters, were dominated (>70%) by fish that had originated from the spawning aggregations and nursery habitat within PPB. Dependence of this broadly dispersed fishery on replenishment from heavily targeted spawning aggregations within one embayment has significant implications for management and monitoring programmes. © 2011 The Authors. Journal of Fish

  1. Temperate marine protected area provides recruitment subsidies to local fisheries.

    Science.gov (United States)

    Le Port, A; Montgomery, J C; Smith, A N H; Croucher, A E; McLeod, I M; Lavery, S D

    2017-10-25

    The utility of marine protected areas (MPAs) as a means of protecting exploited species and conserving biodiversity within MPA boundaries is supported by strong empirical evidence. However, the potential contribution of MPAs to fished populations beyond their boundaries is still highly controversial; empirical measures are scarce and modelling studies have produced a range of predictions, including both positive and negative effects. Using a combination of genetic parentage and relatedness analysis, we measured larval subsidies to local fisheries replenishment for Australasian snapper ( Chrysophrys auratus : Sparidae) from a small (5.2 km 2 ), well-established, temperate, coastal MPA in northern New Zealand. Adult snapper within the MPA contributed an estimated 10.6% (95% CI: 5.5-18.1%) of newly settled juveniles to surrounding areas (approx. 400 km 2 ), with no decreasing trend in contributions up to 40 km away. Biophysical modelling of larval dispersal matched experimental data, showing larvae produced inside the MPA dispersed over a comparable distance. These results demonstrate that temperate MPAs have the potential to provide recruitment subsidies at magnitudes and spatial scales relevant to fisheries management. The validated biophysical model provides a cost-efficient opportunity to generalize these findings to other locations and climate conditions, and potentially informs the design of MPA networks for enhancing fisheries management. © 2017 The Author(s).

  2. The application of hCG, CPH and Ovopel in successful artificial reproduction of goldfish (Carassius auratus auratus) under controlled conditions.

    Science.gov (United States)

    Targońska, K; Kucharczyk, D

    2011-08-01

    Artificial reproduction of fish is one of the main goals of aquaculture production. The aim of this study is to optimize the method of goldfish reproduction under controlled conditions by comparing the effectiveness of carp pituitary homogenate (CPH), Ovopel and human chorionic gonadotropin (hCG), administered as a one-off dose and inducing two spawns in the same fish within a short time period. Goldfish spawners were stimulated with hCG, CPH and Ovopel, and the results were compared to the fish from the control group, comprised of unstimulated fish. In another experiment, spawn were induced twice within an interval of 21 days with the same group of fish. The best results in the first experiment in terms of the percentage of ovulating females and survival to the eyed-egg stage were achieved after administering hCG (100% and 88.7%, respectively). However, the highest fecundity was observed in fish stimulated with Ovopel (89,960 eggs/kg). It was shown in the second experiment that female goldfish produce higher weight of eggs during the first spawning, but the number of eggs/BW ratio was higher during the next reproduction process. Survival, both that of embryos to the eyed-egg stage and that of spawners, is higher during the first reproduction act. © 2010 Blackwell Verlag GmbH.

  3. The effect of the addition of cow brain powder in commercial feed on the gonadal maturity of comet goldfish (Carassius auratus auratus)

    Science.gov (United States)

    Andriani, Y.; Subhan, U.; Rosidah; Iskandar; Zidni, I.; Abdillah, A. M.

    2018-04-01

    The aim of this research was to analysis the effect of addition bovine’s brain meal in artificial feed on gonad maturity and to find out the best time of gonad maturity in comet fish.This research was conducted at Fourth Building Hatchery Faculty of Fisheries and Marine Sciences Padjadjaran University on November 2014 until Januari 2015. Freeze drying of bovine brain was conducted at Research Center Inter University Bandung Institute of Technology. The research was using Completely Randomized Design (CRD) with four treatments and three replications.The treatment were 20 mg/kg, 35 mg/kg, 50mg/kg and control. The parameters of this research are Gonado Somatic Index (GSI) and egg maturity level. Addition of bovine brain meal in feed with the dose of 50 mg/kg are giving the best result until 45 days of the care time against gonad maturity of comet fish with GSI result 12.93 %, egg maturity level ripe phase 21.115 and fecundity 1520 grain/g.

  4. Effects of protein hydrolysates supplementation in low fish meal diets on growth performance, innate immunity and disease resistance of red sea bream Pagrus major.

    Science.gov (United States)

    Khosravi, Sanaz; Rahimnejad, Samad; Herault, Mikaël; Fournier, Vincent; Lee, Cho-Rong; Dio Bui, Hien Thi; Jeong, Jun-Bum; Lee, Kyeong-Jun

    2015-08-01

    This study was conducted to evaluate the supplemental effects of three different types of protein hydrolysates in a low fish meal (FM) diet on growth performance, feed utilization, intestinal morphology, innate immunity and disease resistance of juvenile red sea bream. A FM-based diet was used as a high fish meal diet (HFM) and a low fish meal (LFM) diet was prepared by replacing 50% of FM by soy protein concentrate. Three other diets were prepared by supplementing shrimp, tilapia or krill hydrolysate to the LFM diet (designated as SH, TH and KH, respectively). Triplicate groups of fish (4.9 ± 0.1 g) were fed one of the test diets to apparent satiation twice daily for 13 weeks and then challenged by Edwardsiella tarda. At the end of the feeding trial, significantly (P red sea bream. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Dietary effects of adenosine monophosphate to enhance growth, digestibility, innate immune responses and stress resistance of juvenile red sea bream, Pagrus major.

    Science.gov (United States)

    Hossain, Md Sakhawat; Koshio, Shunsuke; Ishikawa, Manabu; Yokoyama, Saichiro; Sony, Nadia Mahjabin

    2016-09-01

    Our study explored the dietary effects of adenosine monophosphate (AMP) to enhance growth, digestibility, innate immune responses and stress resistance of juvenile red sea bream. A semi-purified basal diet supplemented with 0% (Control), 0.1% (AMP-0.1), 0.2% (AMP-0.2), 0.4% (AMP-0.4) and 0.8% (AMP-0.8) purified AMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (mean initial weight 3.4 g) for 56 days. The results indicated that dietary AMP supplements tended to improve growth performances. One of the best ones was found in diet group AMP-0.2, followed by diet groups AMP-0.1, AMP-0.4 and AMP-0.8. The Apparent digestibility coefficients (dry matter, protein and lipid) also improved by AMP supplementation and the significantly highest dry matter digestibility was observed in diet group AMP-0.2. Fish fed diet groups AMP-0.2 and AMP-0.4 had significantly higher peroxidase and bactericidal activities than fish fed the control diet. Nitro-blue-tetrazolium (NBT) activity was found to be significantly (P  0.05) by dietary supplementation. In contrast, catalase activity decreased with AMP supplementation. Moreover, the fish fed AMP supplemented diets had better improvement (P red sea bream. The regression analysis revealed that a dietary AMP supplementation between 0.2 and 0.4% supported weight gain and lysozyme activity as a marker of immune functions for red sea bream, which is also inline with the most of the growth and health performance parameters of fish under present experimental conditions. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Temperature effects on snapping performance in the common snapper Chelydra serpentina (Reptilia, Testudines).

    Science.gov (United States)

    Vervust, Bart; Brecko, Jonathan; Herrel, Anthony

    2011-01-01

    Studies on the effect of temperature on whole-animal performance traits other than locomotion are rare. Here we investigate the effects of temperature on the performance of the turtle feeding apparatus in a defensive context. We measured bite force and the kinematics of snapping in the Common Snapping Turtle (Chelydra serpentina) over a wide range of body temperatures. Bite force performance was thermally insensitive over the broad range of temperatures typically experienced by these turtles in nature. In contrast, neck extension (velocity, acceleration, and deceleration) and jaw movements (velocity, acceleration, and deceleration) showed clear temperature dependence with peak acceleration and deceleration capacity increasing with increasing temperatures. Our results regarding the temperature dependence of defensive behavior are reflected by the ecology and overall behavior of this species. These data illustrate the necessity for carefully controlling T(b) when carrying out behavioral and functional studies on turtles as temperature affects the velocity, acceleration, and deceleration of jaw and neck extension movements. More generally, these data add to the limited but increasing number of studies showing that temperature may have important effects on feeding and defensive performance in ectotherms. © 2010 Wiley-Liss, Inc.

  7. 77 FR 32914 - Snapper-Grouper Fishery of the South Atlantic; 2012 Recreational Accountability Measure and...

    Science.gov (United States)

    2012-06-04

    ... Accountability Measure and Closure for South Atlantic Golden Tilefish AGENCY: National Marine Fisheries Service...; closure. SUMMARY: NMFS implements accountability measures (AMs) for the recreational sector of golden...

  8. 78 FR 30779 - Snapper-Grouper Fishery of the South Atlantic; 2013 Recreational Accountability Measure and...

    Science.gov (United States)

    2013-05-23

    ... Accountability Measure and Closure for South Atlantic Snowy Grouper AGENCY: National Marine Fisheries Service...; closure. SUMMARY: NMFS implements accountability measures (AMs) for the recreational sector of snowy...

  9. 78 FR 32995 - Snapper-Grouper Fishery of the South Atlantic; 2013 Recreational Accountability Measure and...

    Science.gov (United States)

    2013-06-03

    ... Accountability Measure and Closure for South Atlantic Golden Tilefish AGENCY: National Marine Fisheries Service...; closure. SUMMARY: NMFS implements accountability measures (AMs) for the recreational sector of golden...

  10. 75 FR 62762 - Proposed Information Collection; Comment Request; Socio-Economic Assessment of Snapper Grouper...

    Science.gov (United States)

    2010-10-13

    ... strengthen and improve fishery management decision-making, satisfy legal mandates under Executive Order 12866... statutes. II. Method of Collection The socio-economic information sought will be collected via in- person... Hours: 1,200. Estimated Total Annual Cost to Public: $0. IV. Request for Comments Comments are invited...

  11. 50 CFR 622.16 - Gulf red snapper individual fishing quota (IFQ) program.

    Science.gov (United States)

    2010-10-01

    ... shareholder must retain sufficient shares to account for the allocation that will be deducted the subsequent.... Failure to comply with this advance notice of landing requirement is unlawful and will preclude... not limited to: A locked gate, fence, wall, or other barrier preventing 24-hour access to the site; a...

  12. Snapper Island Extension, Density, and Calcification Data for 1923 to 1984

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Extension, Density, and Calcification data from 35 Porites coral cores covering the entire length of the Great Barrier Reef, Australia. Data set contains 35...

  13. SEAMAP Groundfish/Shrimp Trawl Red Snapper Analytical Dataset Provided to NRDA

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Southeast Fisheries Science Center Mississippi Laboratories has conducted standardized groundfish trawl surveys in the Gulf of Mexico since 1987. Prior to 1987,...

  14. Helminth parasite communities of spotted rose snapper Lutjanus guttatus from the Mexican Pacific

    Directory of Open Access Journals (Sweden)

    Morales-Serna F. N.

    2017-09-01

    Full Text Available The helminth communities of L. guttatus from Mazatlan Bay (MB and Banderas Bay (BB, on the Pacific coast of Mexico, were studied during two consecutive years. A total of 536 fish were collected and 19 parasite taxa registered (six digeneans, two cestodes, nine nematodes, and two monogeneans. Infection levels of common helminth species (Helicometrina nimia, Siphodera vinaledwardsii, Tetraphyllidea gen. sp., Pseudoterranova sp., Ancyrocephalidae gen. sp. and Microcotyloides incisa as well as the infracommunity indices varied significantly between MB and BB, and among dry and rainy seasons; however, no clear seasonal patterns were observed. Pseudoterranova larvae appeared frequently in MB, possibly because of the presence of the California sea lion in this locality. Similarity analysis did not show a clear separation of parasite species composition between both localities, which suggest that fish samples came from a single population of L. guttatus.

  15. Waste utilization of red snapper (Lutjanus sp.) fish bone to improve phosphorus contents in compost

    Science.gov (United States)

    Ramadhani, S.; Iswanto, B.; Purwaningrum, P.

    2018-01-01

    The purpose of this research is to get the idea that bone waste will be the P content enhancer in compost so that the compost produced meets the standard P levels specified in SNI 19-7030-2004 which regulating compost quality standard. Nutrient levels were obtained in fish bone meal (FBM) are C (3.35%), N (0.48%), P (30.90%) and K (0.02%). Effects of fish bone meal to the rising levels of P in the compost has been known. P levels of compost B, C, D, and E increased at 428.57; 542.85; 657.14 and 914.28% against the compost A (blank). FBM ideal addition indicated in compost B, as much as 15 gr, with a P content of 0.37% and has been passed according standards (0.10% for P). C/N ratio decreased over the 21 days period of composting, with the greatest decline was compost E with a ratio of 16:1. Highest nitrogen (N) levels recorded respectively in compost B and C with value of 1.09% and the lowest of recorded N content was compost A, D and E (1.08%). N content in all samples of compost were eligible minimum N of 0.40%. Carbon (C) is the highest recorded in compost B; 20.20% and the lowest in the compost E; 17.34%. Highest and lowest C levels on the compost has met the minimum C of 9.80%. Composting is done in a bucket as an aerobic composter (with air holes), compost pile turnover for each sample is controlled as much as once/2 days. Mesophilic period (23-450C) occurs during the 21-day period of composting. Compost B has P content of 0.37%, so it has fulfilled the provisions of SNI 19-7030-2004 about the recommended compost standard.

  16. Ultraviolet influence on catalase activity and mineral content in eyeballs of gibel carp (Carassius auratus gibelio)

    International Nuclear Information System (INIS)

    Formicki, Grzegorz; Stawarz, Robert

    2006-01-01

    UV radiation present in the environment may induce several eye disorders including inflammation and cataract. The aim of this work was to study catalase activity and Mg, Cu, Ca and Zn contents in the eyeballs of gibel carp exposed to ecologically relevant doses of UV radiation (0.09 kJ/m 2 for UV-A and 0.98 kJ/m 2 for UV-B) simulated in laboratory conditions. Ultraviolet exposure resulted in significant reduction of catalase activity in the eyeballs of tested fish. Reductions in Mg, Cu and Ca contents after UV exposure were also observed. The differences in Mg and Cu levels between control and UV exposed animals were statistically significant, whereas the reduction of Ca level was insignificant. Zinc level in the eyeballs of UV-exposed fish was significantly higher than in non-irradiated specimens. The results suggest that ultraviolet radiation affects prooxidant/antioxidant balance and Mg, Cu, Zn contents in the eyes of fish living in shallow habitats. These may lead to cataract formation

  17. Quantification of fecal estradiol and progesterone metabolites in Syrian hamsters (Mesocricetus auratus

    Directory of Open Access Journals (Sweden)

    Chelini M.O.M.

    2005-01-01

    Full Text Available Alternative methods to the utilization of laboratory animal blood and its by-products are particularly attractive, especially regarding hamsters due to their small size and difficulties in obtaining serial blood samples. Steroid hormone metabolite quantification in feces, widely used in studies of free-ranging or intractable animals, is a non-invasive, non-stressor, economical, and animal saving technique which allows longitudinal studies by permitting frequent sampling of the same individual. The present study was undertaken to determine the suitability of this method for laboratory animals. Estradiol and progesterone metabolites were quantified by radioimmunoassay in feces of intact, sexually mature female Syrian hamsters during the estrous cycle (control and in feces of superovulated females. Metabolites were extracted by fecal dilution in ethanol and quantified by solid phase radioimmunoassay. Median estrogen and progesterone concentrations were 9.703 and 180.74 ng/g feces in the control group, respectively. Peaks of estrogen (22.44 ± 4.54 ng/g feces and progesterone (655.95 ± 129.93 ng/g feces mean fecal concentrations respectively occurred 12 h before and immediately after ovulation, which is easily detected in this species by observation of a characteristic vaginal postovulatory discharge. Median estrogen and progesterone concentrations (28.159 and 586.57 ng/g feces, respectively were significantly higher in superovulated animal feces (P < 0.0001. The present study demonstrated that it is possible to monitor ovarian activity in Syrian hamsters non-invasively by measuring fecal estradiol and progesterone metabolites. This technique appears to be a quite encouraging method for the development of new endocrinologic studies on laboratory animals.

  18. Neural mechanisms of individual and sexual recognition in Syrian hamsters (Mesocricetus auratus).

    Science.gov (United States)

    Petrulis, Aras

    2009-06-25

    Recognizing the individual and sexual identities of conspecifics is critical for adaptive social behavior and, in most mammals this information is communicated primarily by chemosensory cues. Due to its heavy reliance on odor cues, we have used the Syrian hamster as our model species for investigating the neural regulation of social recognition. Using lesion, electrophysiological and immunocytochemical techniques, separate neural pathways underlying recognition of individual odors and guidance of sex-typical responses to opposite-sex odors have been identified in both male and female hamsters. Specifically, we have found that recognition of individual odor identity requires olfactory bulb connections to entorhinal cortex (ENT) rather than other chemoreceptive brain regions. This kind of social memory does not appear to require the hippocampus and may, instead, depend on ENT connections with piriform cortex. In contrast, sexual recognition, through either differential investigation or scent marking toward opposite-sex odors, depends on both olfactory and vomeronasal system input to the corticomedial amygdala. Preference for investigating opposite-sex odors requires primarily olfactory input to the medial amygdala (ME) whereas appropriately targeted scent marking responses require vomeronasal input to ME as well as to other structures. Within the ME, the anterior section (MEa) appears important for evaluating or classifying social odors whereas the posterodorsal region (MEpd) may be more involved in generating approach to social odors. Evidence is presented that analysis of social odors may initially be done in MEa and then communicated to MEpd, perhaps through micro-circuits that separately process male and female odors.

  19. Experimental mixed infection of Leishmania (Leishmania) amazonensis and Leishmania (L.) infantum in hamsters (Mesocricetus auratus).

    Science.gov (United States)

    DE Lima Celeste, Jordanna Luíza; Venuto Moura, Ana Paula; França-Silva, João Carlos; Matos DE Sousa, Gabriela; Oliveira Silva, Soraia; Norma Melo, Maria; Luiz Tafuri, Wagner; Carvalho Souza, Carolina; Monteiro DE Andrade, Hélida

    2017-08-01

    In South America, visceral leishmaniasis is frequently caused by Leishmania infantum and, at an unknown frequency, by Leishmania amazonensis. Therefore, mixed infections with these organisms are possible. Mixed infections might affect the clinical course, immune response, diagnosis, treatment and epidemiology of the disease. Here we describe the clinical course of mixed infections with L. amazonensis and L. infantum in a hamster model. We show that mixed infections are associated with more severe clinical disease than infection with L. amazonensis or L. infantum alone. In spleens with mixed infections, L. infantum outcompeted L. amazonensis in the tissue, but not in culture from tissue. We found increased levels of IgG in animals infected with L. infantum. Although more than 30 bands were revealed in a Western blot, the highest immunogenicity was observed with proteins having molecular masses of 95 and 90 kDa, whereas proteins with molecular masses of lower than 50 kDa were reactive frequently with serum from hamsters infected with L. amazonensis, and proteins with molecular masses of 80 and 70 kDa were reactive only with serum from hamsters infected with L. infantum. This finding has important implications regarding the biology of Leishmania and humoral immune responses to infections with these organisms.

  20. Histochemical study of brown-fat cells in the golden hamster (Mesocricetus auratus) in cultures

    International Nuclear Information System (INIS)

    Sokolov, V.E.; Boyadzhieva-Mikhailova, A.; Koncheva, L.; Angelova, P.; Evgen'eva, T.P.

    1985-01-01

    The authors undertake the task of studying the synthesis of certain hormones by brown-fat cells. The authors used brown-fat cells from the golden hamster. The metabolism of brown-fat cells was studied on precultured cells, which made it possible to detect the synthesis of the studied substances rather than their accumulation in the organ. The authors conducted three experiments. First, fragments of brown fat were cultivated in diffusion chambers in vivo. Pieces of brown fat were cultivated in parallel in vitro on agar (organotypic cultures) and on plasma (histotypic cultures). During cultivation in diffusion chambers, the chambers were implanted in the abdominal cavity of young white rats. For in vitro cultivation, TCM 199 plus 15-20% calf serum was used. A total of 36 cultures with 12 cultures in each series of experiments were performed. The auto-radiographic studies of brown-fat cells were conducted on 24-hour cultures and on brown-fat fragments taken from the intact animal. The cultures were incubated with isotopes for 1 h. Either [ 3 H]lysine (87.3 Ci/mM specific activity), [ 3 H]arginine (16.7 Ci/mM), [ 3 H]glycerol (43 Ci/mM), or [ 3 H]cholesterol (43 Ci/mM) were added to the medium. After incubation, the cultures were washed three times in pure medium, fixed in Sierra fluid, and embedded in paraffin. The paraffin sections were covered with Ilford K 2 emulsion, and the preparations were exposed for 20 days at 4 0 C temperature. Radio-immunological methods were used to study the accumulation of estradiol-17-beta in the culture medium by the Dobson method and that of testerone. The culture medium was taken on cultivation days 2,4,6,8, and 10. The medium was changed during cultivation every third day, which made it possible to judge the rates of accumulation of material with increase in the cultivation times

  1. Study of goldfish (Carassius auratus) growth hormone structure-function relationship by domain swapping.

    Science.gov (United States)

    Chan, Y H; Cheng, C H K; Chan, K M

    2007-03-01

    Using goldfish as a model, the structure-function relationship of goldfish growth hormone was studied using the strategy of homologous domain swapping. Chimeric mutants were constructed by exchanging homologous regions between goldfish growth hormone (gfGH II) and goldfish prolactin (gfPRL) with their cloned complementary DNAs. Six mutants, with their domain-swapped, were generated to have different combinations of three target regions, including the helix a, helix d and the large section in between these helices (possess the helices b, c and other random coiled regions). After expression in E. coli and refolding, these mutants were characterized by using competitive receptor binding assay (RRA) and growth hormone responding promoter activation assay. The different activity profiles of mutants in Spi 2.1 gene promoter assays from that in RRA shows that, for gfGH, receptor binding dose not confer receptor signal activations. When either helices a or d of gfGH was maintained with other helices replaced by their gfPRL counterparts, both receptor binding and hence gene activation activities are reduced. In mutants with helices b and c in gfGH maintained, containing the gfGH middle section, and helices a and d swapped with gfPRL, the had reduced RRA activities but the promoter activation activities retained. In conclusion, as in the case of human GH, the gfGH molecule possesses two functional sites: one of them is composed of discontinuous epitopes located on the target regions of this study and is for receptor binding; another site is located on the middle section of the molecule that helices a and d are not involved, and it is for activation of GH receptor and intracellular signals.

  2. Foraging Behavior in Golden Hamsters (Mesocricetus Auratus: Effect of the Distance among Multiple Patches

    Directory of Open Access Journals (Sweden)

    Felipe Cabrera

    2008-05-01

    Full Text Available The pattern of travel and the efficiency in foraging behavior was evaluated in four hamsters searching for food within an enclosure with multiple patches. Two different distances among patches were randomly arranged: Near-Patches (10 cm separation and Distant-Patches (21.5 cm separation. Subjects obtained the food by mounting over the cylinders (stations placed in the enclosure of 110 cm2. Results showed that in both, Near and Distant conditions, the distance between responses was longer in late stages of the trials then in early stages. Nonetheless, the most choices to adjacent stations were in Distant-Patches condition, while skips and diagonal-station choices were more frequently showed in the Near-Patches condition.

  3. Morphogenesis of palatal ridges in the golden hamster (Mesocricetus auratus, Rodentia)

    Czech Academy of Sciences Publication Activity Database

    Buchtová, M.; Matulová, Petra; Witter, K.; Tichý, F.

    2005-01-01

    Roč. 74, č. 3 (2005), s. 319-327 ISSN 0001-7213 R&D Projects: GA MŠk OC B23.001 Institutional research plan: CEZ:AV0Z50450515 Keywords : oral cavity Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 0.353, year: 2005

  4. Melatonin synthesis under calcium constraint in gilthead sea bream (Sparus auratus L.)

    NARCIS (Netherlands)

    Abbink, W.; Kulczkowska, E.; Kalamarz, H.; Guerreiro, P.M.G.; Flik, G.

    2008-01-01

    Brain or blood plasma melatonin was analysed as a measure for pineal melatonin production in sea bream. Access to calcium was limited by diluting the seawater to 2.5‰ and removing calcium from the diet or by prolonged feeding of vitamin D-deficient diet. Interactions/relations between melatonin and

  5. The effects of salinity on growth of Goldfish, Carassius auratus and ...

    African Journals Online (AJOL)

    adunet

    2013-04-17

    Apr 17, 2013 ... weight gain, food intake and survival of goldfish and crucian carp were investigated for 20 days. Both ... There are various studies on the effects of these factors ..... have high salt in the blood and do not spend more energy.

  6. Isolation of a novel aquaglyceroporin from a marine teleost (Sparus auratus): function and tissue distribution.

    NARCIS (Netherlands)

    Santos, C.R.; Estevao, M.D.; Fuentes, J.; Cardoso, -; Fabra, M.; Passos, A.L.; Detmers, F.J.M.; Deen, P.M.T.; Cerda, J.; Power, D.M.

    2004-01-01

    The aquaporins (formerly called the major intrinsic protein family) are transmembrane channel proteins. The family includes the CHIP group, which are functionally characterised as water channels and the GLP group, which are specialised for glycerol transport. The present study reports the

  7. Dietary Aloe vera components' effects on cholesterol lowering and estrogenic responses in juvenile goldfish, Carassius auratus.

    Science.gov (United States)

    Palermo, Francesco A; Cocci, Paolo; Angeletti, Mauro; Felici, Alberto; Polzonetti-Magni, Alberta Maria; Mosconi, Gilberto

    2013-08-01

    Aloes are now considered a very interesting source of bioactive compounds among which phytosterols should play a major role. The present study is an attempt to investigate the hypocholesterolemic activity of Aloe vera associated with its impact on the reproductive status of juvenile goldfish. Therefore, the short- and long-term effects of feeding supplementary diet containing aloe components (20 mg aloe/g diet; 2%) on plasma lipids, plasma vitellogenin, and hepatic estrogen receptor α/β1 mRNA levels in goldfish were examined. Results of GC-MS for phytosterols show high abundance of β-sitosterol in freeze-dried powder of Aloe vera whole leaves. Moreover, a 2% aloe powder dietary supplement was not found estrogenic in juvenile goldfish after either 7- or 30-day treatment, but was consistent in plasma hypocholesterolemic effects following long-term exposure. The present data further support that plasma cholesterol modulation induced by phytosterols may not be related to estrogen-like activity.

  8. Exploratory metabolomics study of the experimental opisthorchiasis in a laboratory animal model (golden hamster, Mesocricetus auratus.

    Directory of Open Access Journals (Sweden)

    Daria A Kokova

    2017-10-01

    Full Text Available Opisthorchiasis is a parasitic infection caused by the liver flukes of the Opisthorchiidae family. Both experimental and epidemiological data strongly support a role of these parasites in the etiology of the hepatobiliary pathologies and an increased risk of intrahepatic cholangiocarcinoma. Understanding a functional link between the infection and hepatobiliary pathologies requires a detailed description a host-parasite interaction on different levels of biological regulation including the metabolic response on the infection. The last one, however, remains practically undocumented. Here we are describing a host response on Opisthorchiidae infection using a metabolomics approach and present the first exploratory metabolomics study of an experimental model of O. felineus infection.We conducted a Nuclear Magnetic Resonance (NMR based longitudinal metabolomics study involving a cohort of 30 animals with two degrees of infection and a control group. An exploratory analysis shows that the most noticeable trend (30% of total variance in the data was related to the gender differences. Therefore further analysis was done of each gender group separately applying a multivariate extension of the ANOVA-ASCA (ANOVA simultaneous component analysis. We show that in the males the infection specific time trends are present in the main component (43.5% variance, while in the females it is presented only in the second component and covers 24% of the variance. We have selected and annotated 24 metabolites associated with the observed effects and provided a physiological interpretation of the findings.The first exploratory metabolomics study an experimental model of O. felineus infection is presented. Our data show that at early stage of infection a response of an organism unfolds in a gender specific manner. Also main physiological mechanisms affected appear rather nonspecific (a status of the metabolic stress the data provides a set of the hypothesis for a search of the more specific metabolic markers of the Opisthorchiidae infection.

  9. Opportunity of integrated production of "Ceratophyllum demersum" in a nutrition trial with "Carassius auratus"

    OpenAIRE

    Santana García, Gustavo Julián

    2016-01-01

    [EN]The integrated culture of fish and plant in a recirculated system is called aquaponics, where a mutual benefit have been demonstrated for the cocultured species. Today this practice is still not well known for the most studied consumed freshwater species, and almost nothing has been done for aquarium species. The objective of present work was to study the opportunity of growing one of the most selled aquarium plant coontail (Ceratophyllum demersum) in a coculture with the goldfish (Carass...

  10. Immunocytochemical localization of the glutamate transporter GLT-1 in goldfish (Carassius auratus) retina

    NARCIS (Netherlands)

    Vandenbranden, C. A.; Yazulla, S.; Studholme, K. M.; Kamphuis, W.; Kamermans, M.

    2000-01-01

    Glutamate is the major excitatory neurotransmitter in the retina of vertebrates. Electrophysiological experiments in goldfish and salamander have shown that neuronal glutamate transporters play an important role in the clearance of glutamate from cone synaptic clefts. In this study, the localization

  11. Ultraviolet influence on catalase activity and mineral content in eyeballs of gibel carp (Carassius auratus gibelio)

    Energy Technology Data Exchange (ETDEWEB)

    Formicki, Grzegorz [Cracow Pedagogical Academy, Institute of Biology, Department of Zoology, ul. Podbrzezie 3, 31-054 Cracow (Poland)]. E-mail: formicki@ap.krakow.pl; Stawarz, Robert [Cracow Pedagogical Academy, Institute of Biology, Department of Zoology, ul. Podbrzezie 3, 31-054 Cracow (Poland)

    2006-10-01

    UV radiation present in the environment may induce several eye disorders including inflammation and cataract. The aim of this work was to study catalase activity and Mg, Cu, Ca and Zn contents in the eyeballs of gibel carp exposed to ecologically relevant doses of UV radiation (0.09 kJ/m{sup 2} for UV-A and 0.98 kJ/m{sup 2} for UV-B) simulated in laboratory conditions. Ultraviolet exposure resulted in significant reduction of catalase activity in the eyeballs of tested fish. Reductions in Mg, Cu and Ca contents after UV exposure were also observed. The differences in Mg and Cu levels between control and UV exposed animals were statistically significant, whereas the reduction of Ca level was insignificant. Zinc level in the eyeballs of UV-exposed fish was significantly higher than in non-irradiated specimens. The results suggest that ultraviolet radiation affects prooxidant/antioxidant balance and Mg, Cu, Zn contents in the eyes of fish living in shallow habitats. These may lead to cataract formation.

  12. Effect of temperature on the concentration of plasma gonadotropine and spermatogenesis of the goldfish Carassius auratus

    Energy Technology Data Exchange (ETDEWEB)

    Gillet, C.; Billard, R.; Breton, B.

    1977-01-01

    Concentrations of immunoreactive plasma gonadotropin (c-GtH) were measured for goldfish kept at conditions of temperature held constant or increasing between 10 and 30/sup 0/C, between February and July. At temperatures of 17/sup 0/C or above the levels of plasma c-GtH were significantly (P < 0.005) higher during April and May than at 10/sup 0/C. In April the pituitary concentration of c-GtH was maximum for fish held at 17/sup 0/C or above and the minimum for fish held below 17/sup 0/C, but in June the situation was reversed. A study of the gonads showed that spermatogenesis is faster at 17--24/sup 0/C than at 10/sup 0/C, but at 30/sup 0/C spermatogenic activity was inhibited.

  13. Nitric oxide improves the hemodynamic performance of the hypoxic goldfish (Carassius auratus) heart

    DEFF Research Database (Denmark)

    Imbrogno, Sandra; Capria, C.; Tota, Bruno

    2014-01-01

    Goldfish tolerate prolonged and severe hypoxia, thus representing a well-suited model to study the maintenance of cardiac function when O2 availability represents a limiting factor. Using a working heart preparation, we explored the role of the intracardiac nitric oxide synthase (NOS)- derived...... complemented by Western blotting analysis which revealed increased expressions of NOS and hypoxia inducible factor α(HIF-1α). In conclusion, we demonstrated that intracardiac NO/NOS enhances goldfish heart performance, remarkably expanding its hypoxic tolerance....

  14. Electrophysiological characterization of male goldfish (Carassius auratus ventral preoptic area neurons receiving olfactory inputs

    Directory of Open Access Journals (Sweden)

    Wudu E. Lado

    2014-06-01

    Full Text Available Chemical communication via sex pheromones is critical for successful reproduction but the underlying neural mechanisms are not well-understood. The goldfish is a tractable model because sex pheromones have been well-characterized in this species. We used male goldfish forebrain explants in vitro and performed whole-cell current clamp recordings from single neurons in the ventral preoptic area (vPOA to characterize their membrane properties and synaptic inputs from the olfactory bulbs (OB. Principle component and cluster analyses based on intrinsic membrane properties of vPOA neurons (N = 107 revealed five (I-V distinct cell groups. These cells displayed differences in their input resistance (Rinput: I II = IV > III = V. Evidence from electrical stimulation of the OB and application of receptor antagonists suggests that vPOA neurons receive monosynaptic glutamatergic inputs via the medial olfactory tract, with connectivity varying among neuronal groups [I (24%, II (40%, III (0%, IV (34% and V (2%].

  15. GABAergic projections to the oculomotor nucleus in the goldfish (Carassius auratus

    Directory of Open Access Journals (Sweden)

    M. Angeles eLuque

    2011-02-01

    Full Text Available The mammalian oculomotor nucleus receives a strong -aminobutyric acid (GABAergic synaptic input, whereas such projections have rarely been reported in fish. In order to determine whether this synaptic organization is preserved across vertebrates, we investigated the GABAergic projections to the oculomotor nucleus in the goldfish by combining retrograde transport of biotin dextran amine, injected into the antidromically identified oculomotor nucleus, and GABA immunohistochemistry. The main source of GABAergic afferents to the oculomotor nucleus was the ipsilateral anterior octaval nucleus, with only a few, if any, GABAergic neurons being located in the contralateral tangential and descending nuclei of the octaval column. In mammals there is a nearly exclusive ipsilateral projection from vestibular neurons to the oculomotor nucleus via GABAergic inhibitory inputs; thus, the vestibulooculomotor GABAergic circuitry follows a plan that appears to be shared throughout the vertebrate phylogeny. The second major source of GABAergic projections was the rhombencephalic reticular formation, primarily from the medial area but, to a lesser extent, from the inferior area. A few GABAergic oculomotor projecting neurons were also observed in the ipsilateral nucleus of the medial longitudinal fasciculus. The GABAergic projections from neurons located in both the reticular formation surrounding the abducens nucleus and the nucleus of the medial reticular formation have primarily been related to the control of saccadic eye movements. Finally, all retrogradely labeled internuclear neurons of the abducens nucleus, and neurons in the cerebellum (close to the caudal lobe, were negative for GABA. These data suggest that the vestibuloocular and saccadic inhibitory GABAergic systems appear early in vertebrate phylogeny to modulate the firing properties of the oculomotor nucleus motoneurons.

  16. Bait effects in sampling coral reef fish assemblages with stereo-BRUVs.

    Science.gov (United States)

    Dorman, Stacey R; Harvey, Euan S; Newman, Stephen J

    2012-01-01

    Baited underwater video techniques are increasingly being utilised for assessing and monitoring demersal fishes because they are: 1) non extractive, 2) can be used to sample across multiple habitats and depths, 3) are cost effective, 4) sample a broader range of species than many other techniques, 5) and with greater statistical power. However, an examination of the literature demonstrates that a range of different bait types are being used. The use of different types of bait can create an additional source of variability in sampling programs. Coral reef fish assemblages at the Houtman Abrolhos Islands, Western Australia, were sampled using baited remote underwater stereo-video systems. One-hour stereo-video recordings were collected for four different bait treatments (pilchards, cat food, falafel mix and no bait (control)) from sites inside and outside a targeted fishery closure (TFC). In total, 5209 individuals from 132 fish species belonging to 41 families were recorded. There were significant differences in the fish assemblage structure and composition between baited and non-baited treatments (Pcat food and pilchards contained similar ingredients and were found to record similar components of the fish assemblage. There were no significant differences in the fish assemblages in areas open or closed to fishing, regardless of the bait used. Investigation of five targeted species indicated that the response to different types of bait was species-specific. For example, the relative abundance of Pagrus auratus was found to increase in areas protected from fishing, but only in samples baited with pilchards and cat food. The results indicate that the use of bait in conjunction with stereo-BRUVs is advantageous. On balance, the use of pilchards as a standardised bait for stereo-BRUVs deployments is justified for use along the mid-west coast of Western Australia.

  17. Bait effects in sampling coral reef fish assemblages with stereo-BRUVs.

    Directory of Open Access Journals (Sweden)

    Stacey R Dorman

    Full Text Available Baited underwater video techniques are increasingly being utilised for assessing and monitoring demersal fishes because they are: 1 non extractive, 2 can be used to sample across multiple habitats and depths, 3 are cost effective, 4 sample a broader range of species than many other techniques, 5 and with greater statistical power. However, an examination of the literature demonstrates that a range of different bait types are being used. The use of different types of bait can create an additional source of variability in sampling programs. Coral reef fish assemblages at the Houtman Abrolhos Islands, Western Australia, were sampled using baited remote underwater stereo-video systems. One-hour stereo-video recordings were collected for four different bait treatments (pilchards, cat food, falafel mix and no bait (control from sites inside and outside a targeted fishery closure (TFC. In total, 5209 individuals from 132 fish species belonging to 41 families were recorded. There were significant differences in the fish assemblage structure and composition between baited and non-baited treatments (P<0.001, while no difference was observed with species richness. Samples baited with cat food and pilchards contained similar ingredients and were found to record similar components of the fish assemblage. There were no significant differences in the fish assemblages in areas open or closed to fishing, regardless of the bait used. Investigation of five targeted species indicated that the response to different types of bait was species-specific. For example, the relative abundance of Pagrus auratus was found to increase in areas protected from fishing, but only in samples baited with pilchards and cat food. The results indicate that the use of bait in conjunction with stereo-BRUVs is advantageous. On balance, the use of pilchards as a standardised bait for stereo-BRUVs deployments is justified for use along the mid-west coast of Western Australia.

  18. Gill structural change in response to turbidity has no effect on the oxygen uptake of a juvenile sparid fish.

    Science.gov (United States)

    Cumming, H; Herbert, N A

    2016-01-01

    Turbidity as a result of increased suspended sediments in coastal waters is an environmental stress of worldwide concern. Recent research on fish suggests that detrimental changes to gill structure can occur in turbid waters, with speculation that these alterations diminish fitness variables, such as growth and development, by negatively impacting the O 2 uptake capacity (respiration) of fish. Specifically to address this unknown, the impact of turbid water on the gill structure, somatic growth rate and O 2 uptake rates of a juvenile sparid species ( Pagrus auratus ) was addressed following exposure to five different turbidity treatments (turbidity units) for 30 days. Significant gill structural change was apparent with a progressive increase in turbidity and was quantified as a reduction in lamellar density, as well as an increase in basal hyperplasia, epithelial lifting and increased oxygen diffusion distance across the lamellae. The weight of control fish did not change throughout the experiment, but all fish exposed to turbid waters lost weight, and weight loss increased with nephelometric turbidity units, confirming that long-term turbidity exposure is detrimental to growth productivity. The growth of fish could be impacted in a variety of ways, but the specific hypothesis that structural alteration of the gills impairs O 2 uptake across the gills and limits growth fitness was not supported because there was no measurable difference in the standard metabolic rate, maximal metabolic rate, aerobic metabolic scope or critical oxygen saturation limit of fish measured in clear water after 30 days of exposure. Although impaired O 2 uptake as a result of structurally adjusted gills is unlikely to be the cause of poor fish growth, the exact mechanism by which growth productivity is affected in turbid conditions remains unclear and warrants further investigation.

  19. 77 FR 66744 - Temporary Rule to Increase the Commercial Annual Catch Limit for South Atlantic Yellowtail Snapper

    Science.gov (United States)

    2012-11-07

    ... South Atlantic Comprehensive ACL Amendment and the Generic ACL Amendment to the Red Drum, Reef Fish Resources, Shrimp, and Coral and Coral Reefs FMPs for the Gulf of Mexico (Generic ACL Amendment) (76 FR... (518,270 kg), round weight. The AM implemented through that rule states that the commercial sector will...

  20. 75 FR 76873 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2010-12-09

    ... AGENCY: National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric Administration (NOAA... be applied over the recovery period; however, such adaptive management measures should balance... current available information. It is recognized that fish dealers, restaurants, and other establishments...

  1. 77 FR 27374 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2012-05-10

    ... preventing overfishing, achieving optimum yield, and minimizing impacts to fishing communities. The Council... currently undergoing overfishing and an annual catch limit (ACL) of zero was established through the final... Regulatory Amendment 11 fails to end overfishing of speckled hind and warsaw grouper and that the Council and...

  2. 77 FR 34254 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2012-06-11

    ... undergoing overfishing, and NMFS notified the Council of the red grouper stock status on June 9, 2010. The... Amendment 24 are expected to ensure red grouper overfishing does not occur and the stock rebuilds to target... communities. The preamble to the final rule provides a statement and need for, and the objectives of this rule...

  3. 76 FR 61285 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; South Atlantic Snapper-Grouper...

    Science.gov (United States)

    2011-10-04

    ... recreational ACL overage. This action is necessary to reduce overfishing of the South Atlantic black sea bass... (AMs) be implemented to end overfishing and prevent overfishing from occurring. AMs are management... overfishing, including black sea bass, and AMs if these ACLs are reached or exceeded. The recreational ACL for...

  4. 78 FR 47574 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2013-08-06

    ... benchmark assessment for the stock to be completed in 2014. After the new benchmark assessment is conducted... a new SEDAR benchmark stock assessment for 2014. Until then, the SSC recommended, and the Council... opportunity for the stock to rebuild until the Council can review the new benchmark assessment. Comment 2: The...

  5. 75 FR 82280 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2010-12-30

    ... bycatch mortality of speckled hind and warsaw grouper. Additionally, a new benchmark stock assessment for speckled hind and warsaw grouper is scheduled to be conducted in 2013. If the results of this new stock... be 100 percent, and this is taken into consideration when conducting stock assessments. Comment 9...

  6. Species Profiles: Life Histories and Environmental Requirements of Coastal Fishes and Invertebrates (South Florida): Gray, Lane, Mutton and Yellowtail Snappers.

    Science.gov (United States)

    1986-06-01

    pargo prieto, pargo, criollo , vivaneau sorbe (Cervigon pargo dienton, pargo de piedra, 1966, Fisher 1978) pargo moreno, vivaneau sarde grise, aquadera... criollo , Lutjanus 1970-79. Florida landings, analis. Cent. Invest. Pesq. annual summary 1969-1978. U.S. uba. Nota 2:1-16. Natl. Mar. Fish. Serv. Curr

  7. NOAA TIFF Image - 4m Bathymetric Depth of Red Snapper Research Areas in the South Atlantic Bight, 2010

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains unified Bathymetric Depth GeoTiffs with 4x4 meter cell resolution describing the topography of 15 areas along the shelf edge off the South...

  8. NOAA TIFF Image - 4m Bathymetric Curvature of Red Snapper Research Areas in the South Atlantic Bight, 2010

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains unified Bathymetric Curvature GeoTiffs with 4x4 meter cell resolution describing the topography of 15 areas along the shelf edge off the South...

  9. NOAA TIFF Image - 4m Bathymetric Mean Depth of Red Snapper Research Areas in the South Atlantic Bight, 2010

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains unified Bathymetric Mean Depth GeoTiffs with 4x4 meter cell resolution describing the topography of 15 areas along the shelf edge off the South...

  10. NOAA TIFF Image - 4m Bathymetric Depth Range of Red Snapper Research Areas in the South Atlantic Bight, 2010

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains unified Bathymetric Depth Range GeoTiffs with 4x4 meter cell resolution describing the topography of 15 areas along the shelf edge off the...

  11. NOAA TIFF Image - 4m Multibeam Backscatter for Red Snapper Research Areas in the South Atlantic Bight, 2010

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains unified Multibeam Backscatter GeoTiffs with 4x4 meter cell resolution describing the geomorphology of 15 areas along the shelf edge off the...

  12. 77 FR 16991 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery off the...

    Science.gov (United States)

    2012-03-23

    ... economic, vessel safety, and social perspectives. Derby-style harvesting, also termed ``the race for fish... biomass of the stock is above the minimum stock size threshold (MSST), which is the level that triggers an... less interaction with protected species, future restrictions imposed on the fishery may also lessen...

  13. 77 FR 32408 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2012-06-01

    ..., Magnuson-Stevens Act requirements, the social and economic environment, and trends in effort which are... landings reach the appropriate level to trigger the AMs. Comment 15: Two recreational anglers state that... brought to shore each day is not necessary to reduce the risk of right whale interactions with black sea...

  14. 77 FR 74119 - Snapper-Grouper Fishery of the South Atlantic; 2012 Commercial Accountability Measure and Closure...

    Science.gov (United States)

    2012-12-13

    ... Accountability Measure and Closure for South Atlantic Snowy Grouper AGENCY: National Marine Fisheries Service...; closure. SUMMARY: NMFS implements accountability measures (AMs) for the commercial sector for snowy...

  15. 76 FR 66273 - Snapper-Grouper Fishery Off the Southern Atlantic States and Coral and Coral Reefs Fishery in the...

    Science.gov (United States)

    2011-10-26

    ...-Grouper Fishery Off the Southern Atlantic States and Coral and Coral Reefs Fishery in the South Atlantic... the South Atlantic Region and the FMP for Coral, Coral Reefs, and Live/Hard Bottom Habitats of the... Aquariums to collect, with certain conditions, various species of reef fish and live rock in Federal waters...

  16. 78 FR 23858 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery off the...

    Science.gov (United States)

    2013-04-23

    ..., in theory, will result in negative effects on the longline component and positive effects on the hook... ownership changes from individual to corporate or vice versa, must document his or her continuity of... procedures of the NOAA Finance Handbook for determining the administrative costs of each special product or...

  17. Human health risk assessment of pesticide residues in snappers (Lutjanus) fish from the Navachiste Lagoon complex, Mexico.

    Science.gov (United States)

    Granados-Galván, Ingrid Alejandra; Rodríguez-Meza, Durga Guadalupe; Luna-González, Antonio; González-Ocampo, Héctor Abelardo

    2015-08-15

    Organochlorine pesticides (OCPs) residues were determined in fillets of Lutjanus colorado, L. argentiventris, and L. novemfasciatus. Fillet samples were collected bimonthly from February 2012 to February 2013. OCPs average concentrations do not differ significantly according to size, weight, or season, nor do they relate with the physico-chemical parameters of the sea water. The highest concentration and most frequently encountered OCPs were endosulfan sulfate, δ-HCH, and heptachlor epoxide, which indicates their use in the recent past and confirms their persistence. Average concentrations of ∑HCHs, ∑chlordane, and ∑heptachlor in samples were above cancer MRLs according to data from monthly consumed portions. HCHs and heptachlor are listed in Appendix III of the Rotterdam Convention of chemicals placed on a prior informed consent procedure for import and export purposes; they are considered illegal in Mexico. The OCPs concentrations above cancer MRLs in Lutjanus spp. turn its frequent consumption into a human health risk. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. 78 FR 26740 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2013-05-08

    ... rule and the results of the SEDAR 17 update, the Council's Scientific and Statistical Committee (SSC...] 0 2. In Sec. 622.183, paragraph (b)(4) is removed and reserved. 0 3. In Sec. 622.190, introductory...

  19. 77 FR 59129 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2012-09-26

    ... landings history from April 16, 2006, through January 14, 2011. Comments and Responses NMFS received a..., including those in the recreational sector, restaurant and seafood businesses, and a consulting firm... addition to 50-percent landings history for the total wreckfish landings from April 16, 2006, through...

  20. 78 FR 46925 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery off the...

    Science.gov (United States)

    2013-08-02

    ... preparation of a draft environmental impact statement (EIS) to an EA for Regulatory Amendment 14. FOR FURTHER... of No Significant Impact (FONSI), the EA and FONSI will be the final environmental documents required by NEPA. If the EA reveals that significant environmental impacts may be reasonably expected to...

  1. 76 FR 12605 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2011-03-08

    ... interest. This correcting amendment includes minor, non-substantive changes to regulatory text to clarify... the FMP. These amendments do not modify, add or remove any rights, privileges or obligations of any... business plans. Because this is a minor technical amendment that will benefit the affected entities, public...

  2. 78 FR 58249 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2013-09-23

    ... years. For the 2013-2014, 2014- 2015, and 2015-2016 fishing years, the net present value of increased... net present value of these profit increases will be approximately $930,000, of [[Page 58252

  3. 77 FR 19169 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2012-03-30

    ..., but the magnitude could be lower or higher. The third alternative for a recreational sector AM would... recreational sector annual catch limits (ACLs); establish red grouper sector accountability measures (AMs); and... sector ACLs and AMs. The intent of this rule is to specify ACLs and AMs for red grouper while maintaining...

  4. 77 FR 19165 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2012-03-30

    ... maximizing harvest potential and not exceeding the ACL. Management Measures Contained in This Proposed Rule... fisheries, and thus earned no gross revenue or profit from commercial fishing activities, between 2006 and... information regarding their profits is currently not available. Seven of the 20 shareholders had at least 1 lb...

  5. 75 FR 49447 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery off the...

    Science.gov (United States)

    2010-08-13

    ... vessels. Depending on the physical structure of a fish's mouth and the way that they take bait, the circle... until modified. OY at equilibrium would be 2,425,000 lb (1,099,961 kg) whole weight. The proposed action... set F OY at a level equivalent to 85 percent F 40%SPR such that OY at equilibrium equals 2,196,000 lb...

  6. 77 FR 75093 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Snapper-Grouper Fishery Off the...

    Science.gov (United States)

    2012-12-19

    ... would not eliminate the underlying incentive to ``race to fish.'' With this incentive remaining intact..., would be transitory. The incentive to ``race to fish'' is still intact so that effort from qualifying... longline component to the hook-and-line component. This, in theory, would result in negative effects on the...

  7. Interaction effects of dietary supplementation of heat-killed Lactobacillus plantarum and β-glucan on growth performance, digestibility and immune response of juvenile red sea bream, Pagrus major.

    Science.gov (United States)

    Dawood, Mahmoud A O; Koshio, Shunsuke; Ishikawa, Manabu; Yokoyama, Saichiro

    2015-07-01

    Both heat-killed Lactobacillus plantarum (HK-LP) and β-glucan (BG) play important roles in growth performance, feed utilization and health status of fish. Therefore, a feeding trial was conducted to determine the interactive effects of dietary HK-LP and BG on growth performance, digestibility, oxidative status and immune response of red sea bream for 56 days. A significant interaction was found between HK-LP and BG on final body weight, total plasma protein, glucose, serum bactericidal activity (BA), total serum protein, serum alternative complement pathway (ACP) activity, protein and dry matter digestibility coefficients (P 0.05). Interestingly, fish fed with both HK-LP at (0.025 and 0.1%) in combination with BG at (0 and 0.1%) showed higher oxidative stress resistance. Under the experimental conditions, dietary HK-LP and BG had a significant interaction on enhancing the growth, digestibility and immune responses of red sea bream. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Efficacy of UV-irradiated larval vaccine of Ancylostoma ceylanicum (Looss, 1911) in golden hamsters (Merocricetus auratus)

    International Nuclear Information System (INIS)

    Menon, S.; Bhopale, M.K.

    1985-01-01

    A vaccination trial in golden hamsters with UV-irradiated infective larvae of Ancylostoma ceylanicum was attempted. One oral vaccination of hamsters with 100 infective larvae irradiated by means of UV-tube (390 nm) at different time intervals induced the development of resistance. As the time exposure of irradiation was increased, there was a corresponding decrease in the subsequent worm establishment. A high level of protection afforded by larvae irradiated for 15 min UV-exposure was recorded giving 99.0% and 95.0% worm reduction against the challenge doses of 100 and 1000 normal larvae respectively. There was no marked difference in worm establishment in hamsters vaccinated either orally or subcutaneously, followed by oral challenge. In the vaccinated hamsters, the manifestations of resistance at 15 min UV-exposure were shown by marked reduction in worm establishment and highly reduced epg in pellets with significantly higher blood haemoglobin levels compared with those given normal larvae as vaccine and challenge controls. (author)

  9. Ultrasonic vocalizations in golden hamsters (Mesocricetus auratus) reveal modest sex differences and nonlinear signals of sexual motivation.

    Science.gov (United States)

    Fernández-Vargas, Marcela; Johnston, Robert E

    2015-01-01

    Vocal signaling is one of many behaviors that animals perform during social interactions. Vocalizations produced by both sexes before mating can communicate sex, identity and condition of the caller. Adult golden hamsters produce ultrasonic vocalizations (USV) after intersexual contact. To determine whether these vocalizations are sexually dimorphic, we analyzed the vocal repertoire for sex differences in: 1) calling rates, 2) composition (structural complexity, call types and nonlinear phenomena) and 3) acoustic structure. In addition, we examined it for individual variation in the calls. The vocal repertoire was mainly composed of 1-note simple calls and at least half of them presented some degree of deterministic chaos. The prevalence of this nonlinear phenomenon was confirmed by low values of harmonic-to-noise ratio for most calls. We found modest sexual differences between repertoires. Males were more likely than females to produce tonal and less chaotic calls, as well as call types with frequency jumps. Multivariate analysis of the acoustic features of 1-note simple calls revealed significant sex differences in the second axis represented mostly by entropy and bandwidth parameters. Male calls showed lower entropy and inter-quartile bandwidth than female calls. Because the variation of acoustic structure within individuals was higher than among individuals, USV could not be reliably assigned to the correct individual. Interestingly, however, this high variability, augmented by the prevalence of chaos and frequency jumps, could be the result of increased vocal effort. Hamsters motivated to produce high calling rates also produced longer calls of broader bandwidth. Thus, the sex differences found could be the result of different sex preferences but also of a sex difference in calling motivation or condition. We suggest that variable and complex USV may have been selected to increase responsiveness of a potential mate by communicating sexual arousal and preventing habituation to the caller.

  10. Ultrasonic vocalizations in golden hamsters (Mesocricetus auratus reveal modest sex differences and nonlinear signals of sexual motivation.

    Directory of Open Access Journals (Sweden)

    Marcela Fernández-Vargas

    Full Text Available Vocal signaling is one of many behaviors that animals perform during social interactions. Vocalizations produced by both sexes before mating can communicate sex, identity and condition of the caller. Adult golden hamsters produce ultrasonic vocalizations (USV after intersexual contact. To determine whether these vocalizations are sexually dimorphic, we analyzed the vocal repertoire for sex differences in: 1 calling rates, 2 composition (structural complexity, call types and nonlinear phenomena and 3 acoustic structure. In addition, we examined it for individual variation in the calls. The vocal repertoire was mainly composed of 1-note simple calls and at least half of them presented some degree of deterministic chaos. The prevalence of this nonlinear phenomenon was confirmed by low values of harmonic-to-noise ratio for most calls. We found modest sexual differences between repertoires. Males were more likely than females to produce tonal and less chaotic calls, as well as call types with frequency jumps. Multivariate analysis of the acoustic features of 1-note simple calls revealed significant sex differences in the second axis represented mostly by entropy and bandwidth parameters. Male calls showed lower entropy and inter-quartile bandwidth than female calls. Because the variation of acoustic structure within individuals was higher than among individuals, USV could not be reliably assigned to the correct individual. Interestingly, however, this high variability, augmented by the prevalence of chaos and frequency jumps, could be the result of increased vocal effort. Hamsters motivated to produce high calling rates also produced longer calls of broader bandwidth. Thus, the sex differences found could be the result of different sex preferences but also of a sex difference in calling motivation or condition. We suggest that variable and complex USV may have been selected to increase responsiveness of a potential mate by communicating sexual arousal and preventing habituation to the caller.

  11. After infection with Leishmania infantum, Golden Hamsters (Mesocricetus auratus) become more attractive to female sand flies (Lutzomyia longipalpis).

    Science.gov (United States)

    Nevatte, T M; Ward, R D; Sedda, L; Hamilton, J G C

    2017-07-21

    In Brazil, human and canine visceral leishmaniasis is caused by infection with Leishmania infantum, a Protist parasite transmitted by blood-feeding female Lutzomyia longipalpis sand flies. The objective of this study was to determine if the odour of hamsters, infected with Le. infantum, was more attractive than the odour of the same hamsters, before they were infected. The attractiveness of odour collected from individual hamsters (n = 13), before they were infected, was compared in a longitudinal study, with the attractiveness of the odour of the same hamster in a Y-tube olfactometer bioassay, at a late stage of infection. The odour of six of the golden hamsters was significantly more attractive to 50% of the female sand flies at the end of infection compared to before infection and the odour of four of the golden hamsters was significantly more attractive to 75% of the female sand flies at the end of infection. These results strongly indicate that hamsters infected with Le. infantum become significantly more attractive to a greater proportion of female sand flies as the infection progresses.

  12. Effect of green light spectra on the reduction of retinal damage and stress in goldfish, Carassius auratus

    International Nuclear Information System (INIS)

    Song, Jin Ah; Kim, Na Na; Choi, Young Jae; Choi, Cheol Young

    2016-01-01

    We investigated the effect of light spectra on retinal damage and stress in goldfish using green (530 nm) and red (620 nm) light emitting diodes (LEDs) at three intensities each (0.5, 1.0, and 1.5 W/m"2). We measured the change in the levels of plasma cortisol and H_2O_2 and expression and levels of caspase-3. The apoptotic response of green and red LED spectra was assessed using the terminal transferase dUTP nick end labeling (TUNEL) assay. Stress indicator (cortisol and H_2O_2) and apoptosis-related genes (caspase-3) decreased in green light, but increased in red light with higher light intensities over time. The TUNEL assay revealed that more apoptotic cells were detected in outer nuclear layers after exposure to red LED over time with the increase in light intensity, than the other spectra. These results indicate that green light efficiently reduces retinal damage and stress, whereas red light induces it. Therefore, red light-induced retina damage may induce apoptosis in goldfish retina. -- Highlights: •Green light efficiently reduces retinal damage and stress. •Green spectra reduce caspase production and apoptosis. •Red light-induced retina damage may induce apoptosis in goldfish retina. •The retina of goldfish recognizes green spectra as a stable environment.

  13. Gonadotropin-inhibitory hormone reduces sexual motivation but not lordosis behavior in female Syrian hamsters (Mesocricetus auratus)

    DEFF Research Database (Denmark)

    Piekarski, David J; Zhao, Sheng; Jennings, Kimberly J

    2013-01-01

    with GnIH or saline. The effect of GnIH on sexual motivation, vaginal scent marking, and lordosis was examined. Following mating, FOS activation was quantified in brain regions implicated in the regulation of female sexual behavior. Intracerebroventricular administration of GnIH reduced sexual motivation...... and vaginal scent marking, but not lordosis behavior. GnIH administration altered FOS expression in key neural loci implicated in female reproductive behavior, including the medial preoptic area, medial amygdala and bed nucleus of the stria terminalis, independent of changes in circulating gonadal steroids...

  14. Effects of various ecological factors on radiostrontium uptake in two euryhaline teleosts: Mugil auratus Risso and Pleuronectes platessa L

    International Nuclear Information System (INIS)

    Amiard, J.-C.

    1975-11-01

    The effects of various ecological, biotic and abiotic factors (age, species, salinity, temperature, sediment, calcium overload, food) on the accumulation of 85 Sr were studied in two euryhaline Teleosts. Generally, all the physico-chemical and biotic factors tending to activate metabolism, slightly increased radiostrontium intake. Concentration factors were seldom above one for animals measured in toto. According to the concentration kinetics of 85 Sr, three types of organs were distinguished: bone-type tissues, soft tissues and digestive tract [fr

  15. Social housing and social isolation: Impact on stress indices and energy balance in male and female Syrian hamsters (Mesocricetus auratus).

    Science.gov (United States)

    Ross, Amy P; Norvelle, Alisa; Choi, Dennis C; Walton, James C; Albers, H Elliott; Huhman, Kim L

    2017-08-01

    Although Syrian hamsters are thought to be naturally solitary, recent evidence from our laboratory demonstrates that hamsters may actually prefer social contact. Hamsters increase their preference for a location associated with an agonistic encounter regardless of whether they have "won" or "lost". It has also been reported that social housing as well as exposure to intermittent social defeat or to a brief footshock stressor increase food intake and body mass in hamsters. By contrast, it has also been suggested that housing hamsters in social isolation causes anxiety-induced anorexia and reductions in body mass selectively in females. The purpose of this study was to determine the physiological consequences of housing hamsters in social isolation versus in social groups. Male and female hamsters were housed singly or in stable groups of 5 for 4weeks after which they were weighed and trunk blood was collected. In addition, fat pads and thymus and adrenal glands were extracted and weighed. Serum and fecal cortisol were measured using an enzyme-linked immunoassay. Housing condition had no effect on serum or fecal cortisol, but socially housed hamsters displayed modest thymus gland involution. Socially housed females weighed more than did any other group, and socially housed females and males had more fat than did socially isolated hamsters. No wounding or tissue damage occurred in grouped hamsters. Overall, these data suggest that Syrian hamsters tolerate both stable social housing and social isolation in the laboratory although social housing is associated with some alteration in stress-related and bioenergetic measures. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. Vegetation Structure of Ebony Leaf Monkey (Trachypithecus auratus) Habitat in Kecubung Ulolanang Nature Preservation Central Java-Indonesia

    Science.gov (United States)

    Ervina, Rahmawati; Wasiq, Hidayat Jafron

    2018-02-01

    Kecubung Ulolanang Nature Preservation is ebony leaf monkey's habitats in Central Java Indonesia. Continuously degradation of their population is caused by illegal hunting and habitat degradation that made this species being vulnerable. Habitat conservation is one of important aspects to prevent them from extinction. The purpose of this research was to analyze the vegetation's structure and composition, which was potentially, becomes habitat and food source for the monkeys. Data collected using purposive sampling with line transect method of four different level of vegetation. Data analysis used Important Value Index and Diversity Index. There were 43 species of vegetation at seedling stage, 18 species at sapling stage, 8 species at poles stage and 27 species at trees stage. Species that had the highest important value index at seedling was Stenochlaena palustri , at the sapling was Gnetum gnemon, at pole was Swietenia mahagoni and at tree was Tectona grandis . Species of trees those were potentially to become habitat (food source) for ebony leaf monkey were T. grandis, Dipterocarpus gracilis, Quercus sundaica and Ficus superba. The highest diversity index was at seedling gwoth stage.

  17. Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish, Carassius auratus.

    Science.gov (United States)

    Suzuki, Nobuo; Sato, Masayuki; Nassar, Hossam F; Abdel-Gawad, Fagr Kh; Bassem, Samah M; Yachiguchi, Koji; Tabuchi, Yoshiaki; Endo, Masato; Sekiguchi, Toshio; Urata, Makoto; Hattori, Atsuhiko; Mishima, Hiroyuki; Shimasaki, Youhei; Oshima, Yuji; Hong, Chun-Sang; Makino, Fumiya; Tang, Ning; Toriba, Akira; Hayakawa, Kazuichi

    2016-08-01

    We have developed an original in vitro bioassay using teleost scale, that has osteoclasts, osteoblasts, and bone matrix as each marker: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Using this scale in vitro bioassay, we examined the effects of seawater polluted with highly concentrated polycyclic aromatic hydrocarbons (PAHs) and nitro-polycyclic aromatic hydrocarbons (NPAHs) on osteoblastic and osteoclastic activities in the present study. Polluted seawater was collected from two sites (the Alexandria site on the Mediterranean Sea and the Suez Canal site on the Red Sea). Total levels of PAHs in the seawater from the Alexandria and Suez Canal sites were 1364.59 and 992.56 ng/l, respectively. We were able to detect NPAHs in both seawater samples. Total levels of NPAHs were detected in the seawater of the Alexandria site (12.749 ng/l) and the Suez Canal site (3.914 ng/l). Each sample of polluted seawater was added to culture medium at dilution rates of 50, 100, and 500, and incubated with the goldfish scales for 6 hrs. Thereafter, ALP and TRAP activities were measured. ALP activity was significantly suppressed by both polluted seawater samples diluted at least 500 times, but TRAP activity did not change. In addition, mRNA expressions of osteoblastic markers (ALP, osteocalcin, and the receptor activator of the NF-κB ligand) decreased significantly, as did the ALP enzyme activity. In fact, ALP activity decreased on treatment with PAHs and NPAHs. We conclude that seawater polluted with highly concentrated PAHs and NPAHs influences bone metabolism in teleosts.

  18. Effect of green light spectra on the reduction of retinal damage and stress in goldfish, Carassius auratus

    Energy Technology Data Exchange (ETDEWEB)

    Song, Jin Ah; Kim, Na Na; Choi, Young Jae; Choi, Cheol Young, E-mail: choic@kmou.ac.kr

    2016-07-22

    We investigated the effect of light spectra on retinal damage and stress in goldfish using green (530 nm) and red (620 nm) light emitting diodes (LEDs) at three intensities each (0.5, 1.0, and 1.5 W/m{sup 2}). We measured the change in the levels of plasma cortisol and H{sub 2}O{sub 2} and expression and levels of caspase-3. The apoptotic response of green and red LED spectra was assessed using the terminal transferase dUTP nick end labeling (TUNEL) assay. Stress indicator (cortisol and H{sub 2}O{sub 2}) and apoptosis-related genes (caspase-3) decreased in green light, but increased in red light with higher light intensities over time. The TUNEL assay revealed that more apoptotic cells were detected in outer nuclear layers after exposure to red LED over time with the increase in light intensity, than the other spectra. These results indicate that green light efficiently reduces retinal damage and stress, whereas red light induces it. Therefore, red light-induced retina damage may induce apoptosis in goldfish retina. -- Highlights: •Green light efficiently reduces retinal damage and stress. •Green spectra reduce caspase production and apoptosis. •Red light-induced retina damage may induce apoptosis in goldfish retina. •The retina of goldfish recognizes green spectra as a stable environment.

  19. Toxicological responses on cytochrome P450 and metabolic transferases in liver of goldfish (Carassius auratus) exposed to lead and paraquat.

    Science.gov (United States)

    Xu, Xiaoming; Cui, Zhaojie; Wang, Xinlei; Wang, Xixin; Zhang, Su

    2018-04-30

    As the producer of reactive oxygen species (ROS), both lead (Pb) and paraquat (PQ) can generate serious oxidative stress in target organs which result in irreversible toxic effects on organisms. They can disturb the normal catalytic activities of many enzymes by means of different toxicity mechanism. The changed responses of enzymes are frequently used as the biomarkers for indicating the relationship between toxicological effects and exposure levels. In this work, goldfish was exposed to a series of test groups containing lead and paraquat in the range of 0.05-10mg/L, respectively. Four hepatic enzyme activities, including 7-ethoxyresorufinO-deethylase (EROD), 7-benzyloxy-4-trifluoromethyl-coumarin-O-debenzyloxylase (BFCOD), glutathione S-transferase (GST) and UDP-glucuronosyltransferase (UGT) were determined after 1, 7, 14, 28 days exposure. The results showed that the activities of EROD and BFCOD in fish were significantly inhibited in response to paraquat at all exposure levels during the whole experiment. Similarly, the inhibitory effects of lead exposure on BFCOD activity were found in our study, while different responses of lead on EROD were observed. There were no significant differences on EROD activity under lower concentrations of lead (less than 0.1mg/L) before 14 days until an obvious increase was occurred for the 0.5mg/L lead treatment group at day 14. Furthermore, lead showed stronger inhibition on GST activity than paraquat when the concentrations of the two toxicants were more than 0.5mg/L. However, the similar dose and time-dependent manners of UGT activity were found under lead and paraquat exposure. Our results indicated that higher exposure levels and longer accumulations caused inhibitory effects on the four enzymes regardless of lead or paraquat stress. In addition, the responses of phase I enzymes were more sensitive than that of phase II enzymes and they may be served as the acceptable biomarkers for evaluating the toxicity effects of both lead and paraquat. Copyright © 2018 Elsevier Inc. All rights reserved.

  20. Radiation-induced acute necrosis of the pancreatic islet and the diabetic syndrome in the golden hamster (Mesocricetus auratus)

    Energy Technology Data Exchange (ETDEWEB)

    Tsubouchi, S; Suzuki, H; Ariyoshi, H [Aichi Cancer Center, Nagoya (Japan); Matsuzawa, T [Tohoku Univ., Sendai (Japan). Research Inst. for Tuberculosis and Cancer

    1981-07-01

    Exposure of golden hamsters to 35 000 rad of X-rays induced acute and specific necrosis of the cells of the islets of Langerhans of the pancreas within 4 hours, whereas no other tissue revealed any drastic changes which would lead to a critical illness until 36 hours. Animals began to show the characteristic signs of diabetes, that is, hyperglycaemia, hyperkalaemia, ketonemia, and acidosis at 12 hours and these continued until death, 56+-8 hours later. These were accompanied by the disappearance of ..beta..-cell granules and a decrease of plasma insulin. Treatment of irradiated animals with injections of insulin resulted in a reduction in high blood glucose and the prolongation of survival time up to 5 days, which is comparable to the survival time when the cause of death is gastrointestinal. It is concluded that this radiation-induced diabetic syndrome resulted from acute necrosis of the cells of the islets of Langerhans, a previously unreported lethal effect of radiation in golden hamsters.

  1. Infección experimental del hámster (Mesocricetus auratus con metacercarias de Paragonimus mexicanus (peruvianus

    Directory of Open Access Journals (Sweden)

    Alina Huiza F

    2001-07-01

    Full Text Available Un hámster hembra adulta infectada oralmente con 11 metacercarias de Paragonimus mexicanus (peruvianus murió a los 10 días de ser infectado. A la autopsia del animal, se observaron y recuperaron 5 ejemplares juveniles de Paragonimus mexicanus (peruvianus aún móviles: 2 en tejido pulmonar, 2 en cavidad toráxica y 1 en tejido graso del peritoneo abdominal.

  2. Vegetation Structure of Ebony Leaf Monkey (Trachypithecus auratus Habitat in Kecubung Ulolanang Nature Preservation Central Java-Indonesia

    Directory of Open Access Journals (Sweden)

    Ervina Rahmawati

    2018-01-01

    Full Text Available Kecubung Ulolanang Nature Preservation is ebony leaf monkey’s habitats in Central Java Indonesia. Continuously degradation of their population is caused by illegal hunting and habitat degradation that made this species being vulnerable. Habitat conservation is one of important aspects to prevent them from extinction. The purpose of this research was to analyze the vegetation’s structure and composition, which was potentially, becomes habitat and food source for the monkeys. Data collected using purposive sampling with line transect method of four different level of vegetation. Data analysis used Important Value Index and Diversity Index. There were 43 species of vegetation at seedling stage, 18 species at sapling stage, 8 species at poles stage and 27 species at trees stage. Species that had the highest important value index at seedling was Stenochlaena palustri , at the sapling was Gnetum gnemon, at pole was Swietenia mahagoni and at tree was Tectona grandis . Species of trees those were potentially to become habitat (food source for ebony leaf monkey were T. grandis, Dipterocarpus gracilis, Quercus sundaica and Ficus superba. The highest diversity index was at seedling gwoth stage.

  3. Vegetation Structure of Ebony Leaf Monkey (Trachypithecus auratus) Habitat in Kecubung Ulolanang Nature Preservation Central Java-Indonesia

    OpenAIRE

    Ervina Rahmawati; Jafron Wasiq Hidayat

    2018-01-01

    Kecubung Ulolanang Nature Preservation is ebony leaf monkey’s habitats in Central Java Indonesia. Continuously degradation of their population is caused by illegal hunting and habitat degradation that made this species being vulnerable. Habitat conservation is one of important aspects to prevent them from extinction. The purpose of this research was to analyze the vegetation’s structure and composition, which was potentially, becomes habitat and food source for the monkeys. Data collected usi...

  4. Freilanduntersuchungen zur Ökologie und zum Verhalten von Trachypithecus auratus kohlbruggei (Haubenlanguren) im West-Bali-Nationalpark, Indonesien

    OpenAIRE

    Vogt, Martina

    2003-01-01

    Languren gehören zu den asiatischen Schlankaffen, die zusammen mit den afrikanischen Stummelaffen die Unterfamilie Colobinae, neben den Cercopithecinae die zweite rezente Gruppe der Cercopithecoidea, bilden. Während heute über viele Vertreter der Cercopithecinae zahlreiche Langzeitstudien zu verschiedenen Jahreszeiten und in unterschiedlichen Habitaten vorliegen, ist über die Biologie der Colobinae noch vergleichsweise wenig bekannt. Die Kenntnisse zur Lebensweise vieler Schlank- und Stummela...

  5. Temperature selection of goldfish (Carassius auratus L. ) and brook trout (Salvelinus fontinalis Mitch. ) after heterogeneous temperature acclimation

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, R.

    1977-01-01

    Goldfish and brook trout were acclimated heterogeneously to temperature by exposing the head and tail simultaneously to different temperatures. The temperature selection of heterogeneously acclimated fish was tested in a vertical temperature gradient and compared to that of homogeneously acclimated fish. All fish selected temperatures essentially corresponding to the state of acclimation of their head. It is concluded that the brain acts as the main control of temperature selection in fish.

  6. Role of pH on the acute toxicity of sulfite in water. [Carassius auratus; Leistes reticulatus

    Energy Technology Data Exchange (ETDEWEB)

    Sano, H.

    1976-01-01

    The toxicity of sulfite to fish decreases with increasing pH value, because the HSO/sub 3//sup -/ ion is more toxic than the SO/sub 3//sup 2 -/ ion. An effective sulfite concentration S/sub eff/ which is proportional to the toxicity on fish is expressed by the following equation: S/sub eff/ = (HSO/sub 3//sup -/) + f(SO/sub 3//sup 2 -/), where f is a coefficient which expresses the change of toxicity of sulfite depending on the pH of the water, and varies for each species of fish. For goldfish, owing to the very small toxic contribution of SO/sub 3//sup 2 -/ ion (f = 0.07), the pH dependence of the toxicity of sulfite on pH was so strong that sulfite seemed almost non-toxic in basic solution. However, f for guppy is somewhat larger (f = 0.20) so that the toxicity of sulfite weakly depends on the pH value of water.

  7. Effects of photoperiod and temperature on the release of prolactin from the pituitary gland of the goldfish, Carassius auratus L

    Energy Technology Data Exchange (ETDEWEB)

    McKeown, B.A. (Simon Fraser Univ., Burnaby, BC); Peter, R.E.

    1976-11-01

    A number of experiments were conducted to investigate the effects of photoperiod and temperature on prolactin release from the goldfish pituitary gland. Fish were acclimated to different photoperiods and temperatures, and also were subjected to a change in either of these two parameters after different acclimation conditions. Serum and pituitary samples were collected and analyzed by radioimmunoassay for prolactin levels. In other experiments samples for prolactin analysis were taken every 3 h intermittently over a period of 3 days from fish that were acclimated to different photoperiod and temperature conditions. Longer photoperiods and higher temperatures caused pituitary prolactin release. Serum prolactin changed on a circadian rhythm and the rhythm was modified depending on the length of the photoperiod.

  8. Behavioral and hormonal changes associated with the infective dose in experimental taeniasis in golden hamsters (Mesocricetus auratus).

    Science.gov (United States)

    Domínguez-Roldan, Rosa; Hallal-Calleros, Claudia; Sciutto, Edda; Hernández, Marisela; Aguirre-Flores, Virginio; García-Jiménez, Sara; Báez-Saldaña, Armida; Flores-Pérez, Fernando Iván

    2016-07-01

    It has been reported that behavioral changes relate to infection in different parasitoses. However, the relation between the extent of the behavioral changes and the magnitude of the infection has been scarcely studied. The aim of this study was to evaluate the relationship between different doses of infection and the behavioral changes induced in the experimental Taenia pisiformis taeniasis in golden hamsters. Groups of nine hamsters were infected with three or six T. pisiformis metacestodes. The locomotor activity was quantified daily in an open field test during the 21 days after infection; anxiety test was performed in an elevated plus-maze with a dark/light area at 7, 14 and 21 days post-infection, and serum cortisol levels were determined by radioimmunoassay before infection and at day 22 after infection. The challenge itself induced modifications on behavior and cortisol levels in hamsters, with or without successful infection (taenia development). Animals challenged with three metacestodes induced a decrease in locomotor activity and an increase in anxiety in infected animals. A higher and earlier decrease in locomotor activity and increased anxiety levels were observed in hamsters challenged with six cysticerci, which were accompanied by higher levels of sera cortisol at the end of the experiment. At necropsy, 44-55% of hamster became infected with an efficiency of implantation of 22-26%, challenged with three or six cysticerci respectively. The challenge of hamsters with metacestodes, promote behavioral changes in an extent dependent on the magnitude of the challenge, disregarding the effectiveness of the infection. Copyright © 2016 Elsevier Inc. All rights reserved.

  9. 76 FR 34656 - Fisheries of the Caribbean; Southeast Data, Assessment, and Review (SEDAR); Public Meeting

    Science.gov (United States)

    2011-06-14

    ... status of the stock, estimates biological benchmarks, projects future population conditions, and... evaluate stock status, estimate population benchmarks and stock status criteria, and project future.... SUMMARY: The SEDAR assessments of the Caribbean stocks of silk snapper, queen snapper and redtail...

  10. 78 FR 18961 - Gulf of Mexico Fishery Management Council; Public Meetings

    Science.gov (United States)

    2013-03-28

    ... Management for Recreational Red Snapper; discuss Red Snapper 5-year IFQ Review and IFQ Inter-sector Trading... Migratory Pelagic Zones; and, receive a summary of South Atlantic Council Motions and Recommendations for...

  11. 78 FR 46820 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Reef Fish Fishery of the Gulf of...

    Science.gov (United States)

    2013-08-02

    ... Institute (FWRI) conducted a benchmark stock assessment of yellowtail snapper. The assessment indicated that... recreational bag limit, revises the yellowtail snapper stock annual catch limit (ACL), and removes the... minimize the opportunity for the vermilion snapper stock ACL to be exceeded by slowing the rate of...

  12. Results of calculations of external gamma radiation exposure rates from fallout and the related radionuclide compositions. Operation Tumbler-Snapper, 1952

    International Nuclear Information System (INIS)

    Hicks, H.G.

    1981-07-01

    This report presents data on calculated gamma radiation exposure rates and ground deposition of related radionuclides resulting from Events that deposited detectable radioactivity outside the Nevada Test Site complex

  13. NOAA TIFF Image - 4m Bathymetric Principal Component Analysis (PCA) of Red Snapper Research Areas in the South Atlantic Bight, 2010

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains unified Bathymetric PCA GeoTiffs with 4x4 meter cell resolution describing the topography of 15 areas along the shelf edge off the South...

  14. A new species of Dentiphilometra (Nematoda: Philometridae) from the musculature of the gray snapper Lutjanus griseus (Osteichthyes) off the Caribbean coast of Mexico

    Czech Academy of Sciences Publication Activity Database

    González-Solís, D.; Moravec, František; Tuz Paredes, V. M.

    2007-01-01

    Roč. 93, č. 6 (2007), s. 1132-1135 ISSN 0022-3395 R&D Projects: GA ČR(CZ) GA524/06/0170; GA MŠk LC522 Institutional research plan: CEZ:AV0Z60220518 Keywords : parasitic nematode * Dentiphilometra * Mexico Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 1.129, year: 2007

  15. Groundfish/Shrimp and Red Snapper trawl surveys conducted in the Gulf of Mexico from 1990-01-01 to 2014-12-30 (NCEI Accession 0147703)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Southeast Fisheries Science Center Mississippi Laboratories has conducted standardized groundfish trawl surveys in the Gulf of Mexico since 1987. Prior to 1987,...

  16. Investigating bomb radiocarbon transport in the southern Pacific Ocean with otolith radiocarbon

    Science.gov (United States)

    Grammer, G. L.; Fallon, S. J.; Izzo, C.; Wood, R.; Gillanders, B. M.

    2015-08-01

    To explore the transport of carbon into water masses from the surface ocean to depths of ∼ 1000 m in the southwest Pacific Ocean, we generated time series of radiocarbon (Δ14C) from fish otoliths. Otoliths (carbonate earstones) from long-lived fish provide an indirect method to examine the "bomb pulse" of radiocarbon that originated in the 1950s and 1960s, allowing identification of changes to distributions of 14C that has entered and mixed within the ocean. We micro-sampled ocean perch (Helicolenus barathri) otoliths, collected at ∼ 400- 500 m in the Tasman Sea, to obtain measurements of Δ14C for those depths. We compared our ocean perch Δ14C series to published otolith-based marine surface water Δ14C values (Australasian snapper (Chrysophrys auratus) and nannygai (Centroberyx affinis)) and to published deep-water values (800-1000 m; orange roughy (Hoplostethus atlanticus)) from the southwest Pacific to establish a mid-water Δ14C series. The otolith bomb 14C results from these different depths were consistent with previous water mass results in the upper 1500 m of the southwest Pacific Ocean (e.g. World Ocean Circulation Experiment and Geochemical Ocean Sections Study). A comparison between the initial Δ14C bomb pulse rise at 400-500 m suggested a ventilation lag of 5 to 10 yr, whereas a comparison of the surface and depths of 800-1000 m detailed a 10 to 20 yr lag in the time history of radiocarbon invasion at this depth. Pre-bomb reservoir ages derived from otolith 14C located in Tasman Sea thermocline waters were ∼ 530 yr, while reservoir ages estimated for Tasman Antarctic intermediate water were ∼ 730 yr.

  17. Introduction to Some Species of Argulus (Crustacea: Branchiura ...

    African Journals Online (AJOL)

    PROF HORSFALL

    2017-12-12

    Dec 12, 2017 ... has been found in Asia, Europe, Africa, Australia and. North, Central, and South .... of Culture Cyprinidae in Pen Culture of Anzali. Lagoon. .... PHM; Wendelaar Bonga, SE (1999). Modulation ... auratus auratus) in India. Biolife.

  18. Oxytocin (OT) and arginine-vasopressin (AVP) act on OT receptors and not AVP V1a receptors to enhance social recognition in adult Syrian hamsters (Mesocricetus auratus).

    Science.gov (United States)

    Song, Zhimin; Larkin, Tony E; Malley, Maureen O'; Albers, H Elliott

    2016-05-01

    Social recognition is a fundamental requirement for all forms of social relationships. A majority of studies investigating the neural mechanisms underlying social recognition in rodents have investigated relatively neutral social stimuli such as juveniles or ovariectomized females over short time intervals (e.g., 2h). The present study developed a new testing model to study social recognition among adult males using a potent social stimulus. Flank gland odors are used extensively in social communication in Syrian hamsters and convey important information such as dominance status. We found that the recognition of flank gland odors after a 3min exposure lasted for at least 24h, substantially longer than the recognition of other social cues in rats and mice. Intracerebroventricular injections of OT and AVP prolonged the recognition of flank gland odor for up to 48h. Selective OTR but not V1aR agonists, mimicked these enhancing effects of OT and AVP. Similarly, selective OTR but not V1aR antagonists blocked recognition of the odors after 20min. In contrast, the recognition of non-social stimuli was not blocked by either the OTR or the V1aR antagonists. Our findings suggest both OT and AVP enhance social recognition via acting on OTRs and not V1aRs and that the recognition enhancing effects of OT and AVP are limited to social stimuli. Copyright © 2016 Elsevier Inc. All rights reserved.

  19. Influence of long-term exposure to dietary cadmium on growth, maturation and reproduction of goldfish (subspecies: Prussian carp Carassius auratus gibelio B.)

    International Nuclear Information System (INIS)

    Szczerbik, P.; Mikolajczyk, T.; Sokolowska-Mikolajczyk, M.; Socha, M.; Chyb, J.; Epler, P.

    2006-01-01

    The influence of long-term exposure of goldfish to dietary cadmium (Cd) on its accumulation in tissues, growth, ovarian development, luteinizing hormone (LH) secretion and a response to hormonal stimulation of spawning were evaluated. The study was conducted on four groups of females for the period of 3 years, from the age of 10 weeks to second spawning. Four doses of Cd were applied in the feed: 0 (control group), 0.1, 1 and 10 mg Cd g -1 of feed (wet weight). The highest dose of Cd (10 mg g -1 ) inhibited growth and caused several behavioural effects. In contrast, lower dose of Cd (1 mg g -1 ) stimulated fish growth. The doses of Cd from 0.1 to 1 mg Cd g -1 did not influence ovarian development. The gonado-somatic index (GSI) and histological analysis of ovaries showed no differences in ovarian development between the control group and the groups receiving these doses of Cd. However, in the group receiving the highest Cd dose, GSI decreased. This was associated with persistent, long-lasting elevation of plasma LH levels. Ovulation did not occur in this group. Injections of salmon GnRH-analogue (sGnRHa) alone or with domperidone (a dopamine receptor antagonist) in sexually mature fish caused an increase of LH levels in all groups, although in the group fed with the highest Cd dose the effect was weaker than in the other groups. After the first spawning season, a negative effect of lower Cd doses (0.1 and 1 mg Cd g -1 ) on ovarian recrudescence (rebuilding of ovaries) and on the response to the consecutive hormonal stimulation of spawning was observed (lower number of ovulating females). There was a significantly higher content of Cd in the livers of fish than in their muscles. The results of hormonal stimulation of spawning and histological analysis of ovaries suggest that in goldfish cadmium acts mainly at the level of ovary rather than on the pituitary gland. We suppose that in the natural environment cadmium present in the feed can play an important role in the accumulation of this element in fish tissues and can influence vital physiological processes

  20. Oxidative stress and apoptosis of Carassius auratus lymphocytes induced by nonplanar (PCB153) and coplanar (PCB169) polychlorinated biphenyl congeners in vitro

    Institute of Scientific and Technical Information of China (English)

    ZHANG Jian-ying; ZHANG Hang-jun; NI Wan-min

    2009-01-01

    and the coplanar congener was more cytotoxic than nonplanar congener. This study suggests that cytotoxicity mechanisms of the PCB congeners on fish lymphocytes depend on their planarity and chemical structures.

  1. Lesions that functionally disconnect the anterior and posterodorsal sub-regions of the medial amygdala eliminate opposite-sex odor preference in male Syrian hamsters (Mesocricetus auratus)

    Science.gov (United States)

    Maras, Pamela M.; Petrulis, Aras

    2009-01-01

    In many rodent species, such as Syrian hamsters, reproductive behavior requires neural integration of chemosensory information and steroid hormone cues. The medial amygdala processes both of these signals through anatomically distinct sub-regions; the anterior region (MeA) receives substantial chemosensory input, but contains few steroid receptor-labeled neurons, whereas the posterodorsal region (MePD) receives less chemosensory input, but contains a dense population of steroid receptors. Importantly, these sub-regions have considerable reciprocal connections, and the goal of this experiment was therefore to determine whether interactions between MeA and MePD are required for male hamsters’ preference to investigate female over male odors. To functionally disconnect MeA and MePD, males received unilateral lesions of MeA and MePD within opposite brain hemispheres. Control males received either unilateral lesions of MeA and MePD within the same hemisphere or sham surgery. Odor preferences were measured using a 3-choice apparatus, which simultaneously presented female, male and clean odor stimuli; all tests were done under conditions that either prevented or allowed contact with the odor sources. Under non-contact conditions, males with asymmetrical lesions investigated female and male odors equally, whereas males in both control groups preferred to investigate female odors. Under contact conditions, all groups investigated female odors longer than male odors, although males with asymmetrical lesions displayed decreased investigation of female odors compared to sham males. These data suggest that MeA-MePD interactions are critical for processing primarily the volatile components of social odors and highlight the importance of input from the main olfactory system to these nuclei in the regulation of reproductive behavior. More broadly, these results support the role of the medial amygdala in integrating chemosensory and hormone information, a process that may underlie social odor processing in a variety of behavioral contexts. PMID:19931356

  2. Elevated CO_2 levels increase the toxicity of ZnO nanoparticles to goldfish (Carassius auratus) in a water-sediment ecosystem

    International Nuclear Information System (INIS)

    Yin, Ying; Hu, Zhengxue; Du, Wenchao; Ai, Fuxun; Ji, Rong; Gardea-Torresdey, Jorge L.; Guo, Hongyan

    2017-01-01

    Highlights: • Elevated CO_2 increased the Zn content in suspension by reducing pH value. • Elevated CO_2 led to higher Zn accumulation in fish tissues. • Elevated CO_2 also intensified the oxidative damage to fish induced by nZnO. - Abstract: Concerns about the environmental safety of metal-based nanoparticles (MNPs) in aquatic ecosystems are increasing. Simultaneously, elevated atmospheric CO_2 levels are a serious problem worldwide, making it possible for the combined exposure of MNPs and elevated CO_2 to the ecosystem. Here we studied the toxicity of nZnO to goldfish in a water-sediment ecosystem using open-top chambers flushed with ambient (400 ± 10 μL/L) or elevated (600 ± 10 μL/L) CO_2 for 30 days. We measured the content of Zn in suspension and fish, and analyzed physiological and biochemical changes in fish tissues. Results showed that elevated CO_2 increased the Zn content in suspension by reducing the pH value of water and consequently enhanced the bioavailability and toxicity of nZnO. Elevated CO_2 led to higher accumulation of Zn in fish tissues (increased by 43.3%, 86.4% and 22.5% in liver, brain and muscle, respectively) when compared to ambient. Elevated CO_2 also intensified the oxidative damage to fish induced by nZnO, resulting in higher ROS intensity, greater contents of MDA and MT and lower GSH content in liver and brain. Our results suggest that more studies in natural ecosystems are needed to better understand the fate and toxicity of nanoparticles in future CO_2 levels.

  3. Investigation of de novo cholesterol synthetic capacity in the gonads of goldfish (Carassius auratus exposed to the phytosterol beta-sitosterol

    Directory of Open Access Journals (Sweden)

    MacLatchy Deborah L

    2006-11-01

    Full Text Available Abstract Total and intra-mitochondrial gonadal cholesterol concentrations are decreased in fish exposed to the phytoestrogen beta-sitosterol (beta-sit. The present study examined the potential for beta-sit to disrupt de novo cholesterol synthesis in the gonads of goldfish exposed to 200 microgram/g beta-sit and 10 microgram/g 17beta-estradiol (E2; estrogenic control by intra-peritoneal Silastic® implants for 21 days. The de novo cholesterol synthetic capacity was estimated by incubating gonadal tissue with 14C-acetate for a period of 18 hours, followed by chloroform/methanol lipid extraction and thin layer chromatography (TLC lipid separation. Lipid classes were confirmed using infrared spectroscopy. Plasma testosterone (T and total cholesterol concentration were measured and gonadosomatic index (GSI was calculated. Plasma T was significantly reduced in male beta-sit-treated fish compared to control and E2-treated fish (p

  4. Effects of chlorpyrifos on the transcription of CYP3A cDNA, activity of acetylcholinesterase, and oxidative stress response of goldfish (Carassius auratus).

    Science.gov (United States)

    Ma, Junguo; Liu, Yang; Niu, Daichun; Li, Xiaoyu

    2015-04-01

    Chlorpyrifos (CPF) is the widely used organophosphate pesticide in agriculture throughout the world. It has been found that CPF is relatively safe to human but highly toxic to fish. In this study, acute toxicity of CPF on goldfish was determined and then the transcription of goldfish cytochrome P450 (CYP) 3A was evaluated after 96 h of CPF exposure at concentrations of 15.3 [1/10 50% lethal concentration (LC50 )] or 51 μg L(-1) (1/3 LC50 ) of CPF. Meanwhile, the enzymatic activities of acetylcholinesterase (AChE), superoxide dismutase (SOD), and catalase (CAT), total antioxidant activity (T-AOC), and the contents of malondialdehyde (MDA) in the liver or brain of goldfish were also determined. The results of acute toxicity testing showed that the 96-h LC50 of CPF to the goldfish was 153 μg L(-1) . Moreover, a length sequence of 1243 bp CYP3A cDNA encoding for 413 amino acids from goldfish liver was cloned. Polymerase chain reaction results reveal that CPF exposure downregulates CYP 3A transcription in goldfish liver, suggesting that goldfish CYP 3A may be not involved in CPF bioactivation. Finally, the results of biochemical assays indicate that 96 h of CPF exposure remarkably inhibits AChE activity in fish liver or brain, alters hepatic antioxidant enzyme activities, decreases brain T-AOC, and causes lipid peroxidation in fish liver. These results suggest that oxidative stress might be involved in CPF toxicity on goldfish. Copyright © 2013 Wiley Periodicals, Inc.

  5. Elevated CO{sub 2} levels increase the toxicity of ZnO nanoparticles to goldfish (Carassius auratus) in a water-sediment ecosystem

    Energy Technology Data Exchange (ETDEWEB)

    Yin, Ying; Hu, Zhengxue [State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210046 (China); Du, Wenchao, E-mail: du@nju.edu.cn [State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210046 (China); Ai, Fuxun; Ji, Rong [State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210046 (China); Gardea-Torresdey, Jorge L. [Department of Chemistry, The University of Texas at El Paso, El Paso, TX 79968 (United States); Environmental Science and Engineering PhD program, The University of Texas at El Paso, El Paso, TX 79968 (United States); University of California Center for Environmental Implications of Nanotechnology (UC CEIN), The University of Texas at El Paso, El Paso, TX 79968 (United States); Guo, Hongyan, E-mail: hyguo@nju.edu.cn [State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210046 (China)

    2017-04-05

    Highlights: • Elevated CO{sub 2} increased the Zn content in suspension by reducing pH value. • Elevated CO{sub 2} led to higher Zn accumulation in fish tissues. • Elevated CO{sub 2} also intensified the oxidative damage to fish induced by nZnO. - Abstract: Concerns about the environmental safety of metal-based nanoparticles (MNPs) in aquatic ecosystems are increasing. Simultaneously, elevated atmospheric CO{sub 2} levels are a serious problem worldwide, making it possible for the combined exposure of MNPs and elevated CO{sub 2} to the ecosystem. Here we studied the toxicity of nZnO to goldfish in a water-sediment ecosystem using open-top chambers flushed with ambient (400 ± 10 μL/L) or elevated (600 ± 10 μL/L) CO{sub 2} for 30 days. We measured the content of Zn in suspension and fish, and analyzed physiological and biochemical changes in fish tissues. Results showed that elevated CO{sub 2} increased the Zn content in suspension by reducing the pH value of water and consequently enhanced the bioavailability and toxicity of nZnO. Elevated CO{sub 2} led to higher accumulation of Zn in fish tissues (increased by 43.3%, 86.4% and 22.5% in liver, brain and muscle, respectively) when compared to ambient. Elevated CO{sub 2} also intensified the oxidative damage to fish induced by nZnO, resulting in higher ROS intensity, greater contents of MDA and MT and lower GSH content in liver and brain. Our results suggest that more studies in natural ecosystems are needed to better understand the fate and toxicity of nanoparticles in future CO{sub 2} levels.

  6. Effect of adjusting kilovolt peak of the X-ray machine on radiographic-image quality in goldfish(Carassius auratus)

    International Nuclear Information System (INIS)

    Nut Nuanmanee; Dilok Wongsathein; Rutch Khattiya; Atigan Thongtharb; Utumma Maghanemi

    2008-01-01

    Objective: To find optimal kilovolt peak (kVp) for radiographic-image quality in Oranda fish with body width and depth ranging between 3-5 centimeters. Materials and Methods: 18 Oranda fish were divided into 6 groups (3 for each group). Body widths of fish in group 1, 2, and 3 were 3, 4, and 5 centimeters, respectively. Body depths of fish in group 4, 5, and 6 were 3, 4, and 5 centimeters, respectively. After the fish were anesthetized, the radiographic images of group 1, 2, and 3 were taken in lateral position with milliamperes (mA) at 50 mA and duration (sec) for 0.02, 0.05, and 0.05 sec, respectively. Kilovolt peak (kVp) was used in range from 40 to 58. In addition, the radiographic images of group 4, 5, and 6 were taken in dorsoventral position at 50 mA for 0.1 sec with varying kVp from 40 to 62. The quality of radiographic images was evaluated by 5 veterinarians and 5 radiologists. Results: The most appropriate kVp in lateral position with 3, 4 and 5 centimeters in body width were 44, 44, and 48, respectively. In addition, the most appropriate kVp in dorsoventral position with 3, 4, and 5 centimeters in body depth were 46, 48, and 50, respectively. Conclusion: The radiographic-image quality in Oranda fish was depended on the appropriated kVp

  7. Effects of various feeding patterns of Bacillus coagulans on growth performance, antioxidant response and Nrf2-Keap1 signaling pathway in juvenile gibel carp (Carassius auratus gibelio).

    Science.gov (United States)

    Yu, Yebing; Wang, Changhai; Wang, Aimin; Yang, Wenping; Lv, Fu; Liu, Fei; Liu, Bo; Sun, Cunxin

    2018-02-01

    The present study was conducted to evaluate the effects of various Bacillus coagulans feeding patterns on growth, antioxidant parameter and Nrf2 pathway in juvenile gibel carp. The similar size of gibel carp (initial weight: 14.33 ± 0.15 g) were subjected to three levels of B. coagulans supplementation (0, 500, and 1000 mg/kg) and two feeding modes (supplementing B. coagulans continuously or for two days of B. coagulans after 5 days of a basal diet) according to a 3 × 2 factorial design. The fish that were continuously fed 500 mg/kg B. coagulans (P2) and those fed the first basal diet for 5 days followed by 500 mg/kg or 1000 mg/kg B.coagulans for 2 days (P4 or P5) showed higher weight gain rate and specific growth rate than the other groups. Blood respiratory burst (RB), myeloperoxidase (MPO), and anti-superoxide anion free radical (AFASER) activities in the P4 group were higher than those of the control. White blood cell count (WBC), RB activity, MPO activity, and glutathione (GSH) content in the P5 group were also higher than those of the control. A similar higher trend was observed in the gene expressions of NADPH oxidase 2 (NOX2), NFE2-related factor (Nrf2), Kelch-like-ECH-associated protein(Keap1) in the P4 and NOX2, NRF2, CNC homolog 1 (Bach1), peroxiredoxin 2 (Prx2) in the P5 group compared with the control. Additionally, we observed a significantly lower level of plasma aspartate aminotransferase (AST), lower activity of alanine aminotransferase (ALT), a higher level of MPO, higher GPX activity, and increased NRF2 and Prx2 expression were all observed in the P2 treatment group compared with the control. Furthermore, the malondialdehyde (MDA) content in the P2, P3, and P4 groups was lower than that of the control. These results indicate that a diet supplemented with appropriate levels of B.coagulans could improve the growth, immune response, and antioxidant capability of gibel carp. We concluded that the pattern of two days of 500 or 1000 mg/kg B. coagulans after 5 days of a basal diet was recommended for gibel carp. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Efecto de substratos de la tirosinasa sobre el crecimiento del mela noma de Greene del jámster sirio (mesocricetus auratus

    Directory of Open Access Journals (Sweden)

    José Perea Sasiaín

    1966-04-01

    Full Text Available En el proceso de oxidación de la L- tirosina a melanina son notables dos productos intermediarios inestables: la DOPA-quinona y el DOPA cromo (II. Es evidente que estos compuestos no inhiben el crecimiento de las células del melanoma. Sin embargo, Chevremont describió la acción mitotóxica de los compuestos de oxidación de la adrenalina: adrenocromo (III y adrenolutina (IV sobre fibroblastos en cultivo de tejidos. Riley planteó la posibilidad de una "quimioterapia racional" del melanoma utilizando la orto-fenilendiamina. Otros autores han hecho indicaciones al respecto. Personalmente hemos estado interesados en encontrar un substrato del sistema oxidativo fenolásico (tirosinasa del melanocito, capaz de generar productos tóxicos, según el concepto de "sintesis" letal de Peters. Aun cuando la especificidad de la tirosinasa del melanoma es mayor que la de los vegetales, encontramos que es capaz de oxidar rápidamente algunos derivados metilados en la cadena lateral de la DOPA y, más lentamente, ciertos  análogos de la adrenalina.

  9. Influence of long-term exposure to dietary cadmium on growth, maturation and reproduction of goldfish (subspecies: Prussian carp Carassius auratus gibelio B.)

    Energy Technology Data Exchange (ETDEWEB)

    Szczerbik, P. [Department of Ichthyobiology and Fisheries, University of Agriculture, 30-199 Cracow-Mydlniki, ul. Spiczakowa 6 (Poland)]. E-mail: rzbienia@kinga.cyf-kr.edu.pl; Mikolajczyk, T. [Department of Ichthyobiology and Fisheries, University of Agriculture, 30-199 Cracow-Mydlniki, ul. Spiczakowa 6 (Poland); Sokolowska-Mikolajczyk, M. [Department of Ichthyobiology and Fisheries, University of Agriculture, 30-199 Cracow-Mydlniki, ul. Spiczakowa 6 (Poland); Socha, M. [Department of Ichthyobiology and Fisheries, University of Agriculture, 30-199 Cracow-Mydlniki, ul. Spiczakowa 6 (Poland); Chyb, J. [Department of Ichthyobiology and Fisheries, University of Agriculture, 30-199 Cracow-Mydlniki, ul. Spiczakowa 6 (Poland); Epler, P. [Department of Ichthyobiology and Fisheries, University of Agriculture, 30-199 Cracow-Mydlniki, ul. Spiczakowa 6 (Poland)

    2006-05-01

    The influence of long-term exposure of goldfish to dietary cadmium (Cd) on its accumulation in tissues, growth, ovarian development, luteinizing hormone (LH) secretion and a response to hormonal stimulation of spawning were evaluated. The study was conducted on four groups of females for the period of 3 years, from the age of 10 weeks to second spawning. Four doses of Cd were applied in the feed: 0 (control group), 0.1, 1 and 10 mg Cd g{sup -1} of feed (wet weight). The highest dose of Cd (10 mg g{sup -1}) inhibited growth and caused several behavioural effects. In contrast, lower dose of Cd (1 mg g{sup -1}) stimulated fish growth. The doses of Cd from 0.1 to 1 mg Cd g{sup -1} did not influence ovarian development. The gonado-somatic index (GSI) and histological analysis of ovaries showed no differences in ovarian development between the control group and the groups receiving these doses of Cd. However, in the group receiving the highest Cd dose, GSI decreased. This was associated with persistent, long-lasting elevation of plasma LH levels. Ovulation did not occur in this group. Injections of salmon GnRH-analogue (sGnRHa) alone or with domperidone (a dopamine receptor antagonist) in sexually mature fish caused an increase of LH levels in all groups, although in the group fed with the highest Cd dose the effect was weaker than in the other groups. After the first spawning season, a negative effect of lower Cd doses (0.1 and 1 mg Cd g{sup -1}) on ovarian recrudescence (rebuilding of ovaries) and on the response to the consecutive hormonal stimulation of spawning was observed (lower number of ovulating females). There was a significantly higher content of Cd in the livers of fish than in their muscles. The results of hormonal stimulation of spawning and histological analysis of ovaries suggest that in goldfish cadmium acts mainly at the level of ovary rather than on the pituitary gland. We suppose that in the natural environment cadmium present in the feed can play an important role in the accumulation of this element in fish tissues and can influence vital physiological processes.

  10. Roles of nitric oxide, nitrite and myoglobin on myocardial efficiency in trout (Oncorthynchus mykiss) and goldfish (Carassius auratus): implications for hypoxia tolerance

    DEFF Research Database (Denmark)

    Pedersen, Claus Lunde; Faggiano, Serena; Helbo, Signe

    2010-01-01

    The roles of nitric oxide synthase activity (NOS), nitrite and myoglobin (Mb) in the regulation of myocardial function during hypoxia were examined in trout and goldfish, a hypoxia-intolerant and hypoxia-tolerant species, respectively. We measured the effect of NOS inhibition, adrenaline and nitr......The roles of nitric oxide synthase activity (NOS), nitrite and myoglobin (Mb) in the regulation of myocardial function during hypoxia were examined in trout and goldfish, a hypoxia-intolerant and hypoxia-tolerant species, respectively. We measured the effect of NOS inhibition, adrenaline...... in both trout and goldfish myocardium, with trout showing a significant increase in the O2 utilization efficiency, i.e. the ratio of twitch force to O2 consumption, suggesting an increased anaerobic metabolism. NOS inhibition enhanced myocardial O2 consumption and decreased efficiency, indicating...... that mitochondrial respiration is under a tone of NOS-produced NO. When trout myocardial twitch force and O2 consumption are enhanced by adrenaline, this NO tone disappears. Consistent with its conversion to NO, nitrite reduced O2 consumption and increased myocardial efficiency in trout but not in goldfish...

  11. PENGARUH VARIASI PERSENTASE EKSTRAK JANGKRIK (Gryllus sp Walk YANG BERBEDA TERHADAP TINGKAT SURVIVAL RATE BENIH IKAN MAS KOKI (Carrasius auratus L

    Directory of Open Access Journals (Sweden)

    Elrifadah Elrifadah

    2016-10-01

    Full Text Available This study Aims to Determine the effect of different variations on survival seed cicada goldfish. The method used in this study is completely randomized design with 3 treatments (A = 3%, B = 5%, and C = 7% and three replications. From these results Obtained survival rate to 83.0% Obtained treatment A, B Obtained 88.33% and 80.0% Obtained C treatment. Then the results of analysis of variance accept the hypothesis Ho that the percentage variation of different cicada extract (3%, 5% and 7% did not Affect the survival rate of seedlings goldfish. During the last condition of water quality research support for seed viability parameters goldfish.

  12. An annotated list of fish parasites (Isopoda, Copepoda, Monogenea, Digenea, Cestoda, Nematoda) collected from Snappers and Bream (Lutjanidae, Nemipteridae, Caesionidae) in New Caledonia confirms high parasite biodiversity on coral reef fish

    OpenAIRE

    Justine, Jean-Lou; Beveridge, Ian; Boxshall, Geoffrey A; Bray, Rodney A; Miller, Terrence L; Moravec, František; Trilles, Jean-Paul; Whittington, Ian D

    2012-01-01

    Background Coral reefs are areas of maximum biodiversity, but the parasites of coral reef fishes, and especially their species richness, are not well known. Over an 8-year period, parasites were collected from 24 species of Lutjanidae, Nemipteridae and Caesionidae off New Caledonia, South Pacific. Results Host-parasite and parasite-host lists are provided, with a total of 207 host-parasite combinations and 58 parasite species identified at the species level, with 27 new host records. Results ...

  13. An annotated list of fish parasites (Isopoda, Copepoda, Monogenea, Digenea, Cestoda, Nematoda) collected from Snappers and Bream (Lutjanidae, Nemipteridae, Caesionidae) in New Caledonia confirms high parasite biodiversity on coral reef fish.

    Science.gov (United States)

    Justine, Jean-Lou; Beveridge, Ian; Boxshall, Geoffrey A; Bray, Rodney A; Miller, Terrence L; Moravec, František; Trilles, Jean-Paul; Whittington, Ian D

    2012-09-04

    Coral reefs are areas of maximum biodiversity, but the parasites of coral reef fishes, and especially their species richness, are not well known. Over an 8-year period, parasites were collected from 24 species of Lutjanidae, Nemipteridae and Caesionidae off New Caledonia, South Pacific. Host-parasite and parasite-host lists are provided, with a total of 207 host-parasite combinations and 58 parasite species identified at the species level, with 27 new host records. Results are presented for isopods, copepods, monogeneans, digeneans, cestodes and nematodes. When results are restricted to well-sampled reef fish species (sample size > 30), the number of host-parasite combinations is 20-25 per fish species, and the number of parasites identified at the species level is 9-13 per fish species. Lutjanids include reef-associated fish and deeper sea fish from the outer slopes of the coral reef: fish from both milieus were compared. Surprisingly, parasite biodiversity was higher in deeper sea fish than in reef fish (host-parasite combinations: 12.50 vs 10.13, number of species per fish 3.75 vs 3.00); however, we identified four biases which diminish the validity of this comparison. Finally, these results and previously published results allow us to propose a generalization of parasite biodiversity for four major families of reef-associated fishes (Lutjanidae, Nemipteridae, Serranidae and Lethrinidae): well-sampled fish have a mean of 20 host-parasite combinations per fish species, and the number of parasites identified at the species level is 10 per fish species. Since all precautions have been taken to minimize taxon numbers, it is safe to affirm than the number of fish parasites is at least ten times the number of fish species in coral reefs, for species of similar size or larger than the species in the four families studied; this is a major improvement to our estimate of biodiversity in coral reefs. Our results suggest that extinction of a coral reef fish species would eventually result in the coextinction of at least ten species of parasites.

  14. Morphological and genetic analyses of the first record of longrakered trevally, Ulua mentalis (Perciformes: Carangidae) and of the pinjalo snapper, Pinjalo pinjalo (Perciformes: Lutjanidae) in the Odisha coast, Bay of Bengal.

    Science.gov (United States)

    Barik, Tapan K; Swain, Surya N; Sahu, Bijayalaxmi; Tripathy, Bibarani; Acharya, Usha R

    2018-05-01

    Identification of fish species have so far been carried out mostly by classical morpho-taxonomy. In the present study, however, an attempt has been taken to identify two species of fishes Ulua mentalis and Pinjalo pinjalo of order Perciformes which happens to be the first record in Odisha coast Bay of Bengal, India during the year 2015, using DNA barcoding technique for reconfirmation over conventional morpho-taxonomy. During recent past, study of molecular-taxonomical profile of mitochondrial DNA in general and Cytochrome Oxidase subunit I (COI) gene in particular has gained enormous importance for accurate identification of species. In the present study, the partial COI sequence of Ulua mentalis and Pinjalo pinjalo were generated. Analysis using the COI gene produced phylogenetic trees in concurrence with other multi gene studies and we came across the identical phylogenetic relationship considering Neighbor-Joining and Maximum Likelihood tree. Moreover, these molecular data set further testified in Bayesian framework to reevaluate the exact taxonomic groupings within the family. Surprisingly, Ulua mentalis and Pinjalo pinjalo seems to be closely related to their sister taxa.

  15. Hábitos alimenticios del pargo rayado, Lutjanus synagris (Perciformes: Lutjanidae, en la zona norte del Caribe colombiano Food habits of the lane snapper, Lutjanus synagris (Perciformes: Lutjanidae, in the north zone of the Colombian Caribbean

    Directory of Open Access Journals (Sweden)

    Oscar Doncel

    2010-01-01

    Full Text Available Se describen los hábitos alimenticios de Lutjanus synagris, mediante el análisis de contenido estomacal, considerando el espectro trófico a nivel general, por sexo y talla, los aspectos ecológicos de las presas, su distribución espacial y la relación de la dieta con las condiciones del habitat. Se analizaron 148 estómagos, de los cuales el 45,3% estaba lleno y el 54,7% vacío. Las presas más representativas (%N fueron: Portunus spp. (27,3%; n = 44 y Squilla intermedia (10,6%; n = 17. El análisis gravimétrico (%P mostró que entre las categorías con mayor peso se encontraron: Gastropoda (31,8%; 51,7 g y Farfantepenaeus spp. (17,5%o; 28,5 g. La frecuencia de ocurrencia (%>FO mostró que: Portunus spp. (25,8%>; 17 estómagos y S. intermedia (10,5%>; 7 estómagos fueron las presas más frecuentes. Según el %>IIR, las categorías más representativas en la dieta fueron Portunus spp. (43,0%> y Gastropoda (13,4%>. Se identificaron cuatro categorías como presas principales: Portunidae (837,06, Gastropoda (260,29, Squillidae (234,66 y Penaeidae (218,05, dos como presas secundarias: Sicyoniidae (126,35 y Trichiuridae (75,64 y las demás como presas circunstanciales. La distribución espacial de la amplitud del nicho trófico y de las tallas de L. synagris, permitió identificar dos zonas: la primera entre Riohacha y Dibulla con los valores más altos de amplitud del nicho trófico (0,64 a 1,00 denotando predadores generalista y donde se registraron las tallas menores y la segunda, entre Riohacha y Punta Gallinas con los valores más bajos de amplitud del nicho trófico (0,00 a 0,23, donde los individuos mostraron alto grado de especialización en la dieta y las tallas mayores.This work describes the food habits of Lutjanus synagris by means of stomach content analysis, considering the trophic spectrum in general as well as by sex and size, the ecological aspeets of the preys, their spatial distribution, and the relationship of the diet with the habitat conditions. In the analysis of 148 stomachs 45.3%o were found to be full and 54.7%> empty. The most representative preys (%>N were: Portunus spp. (27.3 %>; n = 44 and Squilla intermedia (10.6%>; n = 17, whereas the gravimetric analysis (%>P showed the heaviest preys to be Gastropoda (31.8%>; 51.74 g and Farfantepenaeus spp. (17.5%>; 28.52 g. The frequeney of oceurrence (%>FO showed that Portunus spp. (25.4%>; 17 stomachs and S. intermedia (10.5%>; 7 stomachs were the most frequent preys. According to the IRI (%>, Portunus spp. (43.01%> and Gastropoda (13.38%> were the most representative categories. The diet of L. synagris was found to consist of four types of main preys: Portunidae (837.06, Gastropod (260.29, Squillidae (234.66, and Penaeidae (218.05; and two types of secondary preys: Sicyoniidae (126.35 and Trichiuridae (75.64; with all others being incidental preys. The spatial distribution of the breadth of the trophic niche and the size of L. synagris allowed us to identify two zones. The first, between Riohacha and Dibulla, had the highest niche breadth valúes (0.64-1.00, denoting generalist predators, and the smallest specimens of L. synagris. The second zone, between Riohacha and Punta Gallinas, had the lowest niche breath values (0.00-0.23, indicating a high degree of dietary specialization, and the largest-sized individuals.

  16. An annotated list of fish parasites (Isopoda, Copepoda, Monogenea, Digenea, Cestoda, Nematoda) collected from Snappers and Bream (Lutjanidae, Nemipteridae, Caesionidae) in New Caledonia confirms high parasite biodiversity on coral reef fish

    Czech Academy of Sciences Publication Activity Database

    Justine, J.-L.; Beveridge, I.; Boxshall, G.A.; Bray, R. A.; Miller, T.L.; Moravec, František; Trilles, J.-P.; Whittington, I.D.

    2012-01-01

    Roč. 8, č. 1 (2012), s. 22 ISSN 2046-9063 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : biodiversity * coral reef s * parasites * coextinction * New Caledonia * South Pacific Subject RIV: EG - Zoology

  17. Fish remains from Miocene beds of Višnja vas near Vojnik, Slovenia

    Directory of Open Access Journals (Sweden)

    Aleš Šoster

    2013-06-01

    Full Text Available This paper discusses fossil teeth of sharks (Elasmobranchii, Neoselachii and porgies (Teleostei, Sparidae fromthe Miocene glauconite sandstones of Vi{nja vas near Vojnik. The remains of fish teeth, mostly tooth crowns, belongto cartilaginous fishes of the genera Notorynchus, Carcharias, Carcharoides, Isurus and Cosmopolitodus and to abony fish genus Pagrus.

  18. 76 FR 52638 - Fisheries of the Gulf of Mexico and South Atlantic; Southeast Data, Assessment, and Review (SEDAR...

    Science.gov (United States)

    2011-08-23

    ... stock, estimates biological benchmarks, projects future population conditions, and recommends research... yellowtail snapper. SUMMARY: The technical stock assessments of the Gulf of Mexico stock of menhaden and the southeast U.S. stocks of yellowtail snapper will be reviewed during the Review Workshop. See SUPPLEMENTARY...

  19. 75 FR 28783 - South Atlantic Fishery Management Council; Public Meetings

    Science.gov (United States)

    2010-05-24

    ... event the red snapper benchmark stock assessment allows for modification to the proposed management... (SSCs) regarding Atlantic and Gulf of Mexico stocks of king mackerel and Spanish mackerel. The... snapper, including ending overfishing and rebuilding the stock. Management measures include a closure...

  20. 50 CFR Appendix A to Part 622 - Species Tables

    Science.gov (United States)

    2010-10-01

    ... Table 2 of Appendix A to Part 622—Caribbean Reef Fish Lutjanidae—Snappers Unit 1 Silk snapper, Lutjanus... adscensionis Graysby, Epinephelus cruentatus Creole-fish, Paranthias furcifer Unit 4 Red grouper, Epinephelus... striatus Redspotted hawkfish, Amblycirrhitus pinos Flying gurnard, Dactylopterus volitans Atlantic...

  1. 76 FR 59375 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Amendments to the Queen Conch and...

    Science.gov (United States)

    2011-09-26

    ... sectors; revise the composition of the snapper and grouper complexes; prohibit fishing for and possession of three parrotfish species (midnight, blue, rainbow); establish recreational bag limits for snappers... Portal: http://www.regulations.gov . Follow the instructions for submitting comments. Mail: Bill Arnold...

  2. 78 FR 79674 - Gulf of Mexico Fishery Management Council; Public Meeting

    Science.gov (United States)

    2013-12-31

    ... Limits Monitoring Overview 5. Red Snapper Slot Limit Analysis 6. Alternative Red Snapper ABCs a. based on...: The Gulf of Mexico Fishery Management Council (Council) will hold a meeting of the ABC Control Rule... the individual meeting agendas are as follows: ABC Control Rule Working Group Agenda, Wednesday...

  3. 76 FR 69230 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Comprehensive Ecosystem-Based...

    Science.gov (United States)

    2011-11-08

    ...: PART 622--FISHERIES OF THE CARIBBEAN, GULF, AND SOUTH ATLANTIC 1. The authority citation for part 622... and styles used in the South Atlantic snapper- grouper fishery. (b) Extended reach handle. The... appropriate to secure the range of hook sizes and styles used in the South Atlantic snapper-grouper fishery...

  4. 77 FR 4493 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Comprehensive Ecosystem-Based...

    Science.gov (United States)

    2012-01-30

    ... CARIBBEAN, GULF, AND SOUTH ATLANTIC 0 1. The authority citation for part 622 continues to read as follows... and styles used in the South Atlantic snapper- grouper fishery. (b) Extended reach handle. The... appropriate to secure the range of hook sizes and styles used in the South Atlantic snapper-grouper fishery...

  5. 76 FR 17625 - Fisheries of the South Atlantic; Southeast Data, Assessment, and Review; Public Meeting

    Science.gov (United States)

    2011-03-30

    ...: Participants will present summary data, and discuss data needs and treatments. Special Accommodations The... the South Atlantic; Southeast Data, Assessment, and Review; Public Meeting AGENCY: National Marine... of Southeast Data and Review (SEDAR) 26 data webinar for Caribbean Silk snapper, Queen snapper, and...

  6. 77 FR 36396 - Safety Zones; Fireworks Displays in Captain of the Port Long Island Sound Zone

    Science.gov (United States)

    2012-06-19

    ... viewing or submitting material to the docket, call Renee V. Wright, Program Manager, Docket Operations... Fireworks. South Bay off Snapper Inn Restaurant, Oakdale, NY. 5. North Bay 4th of July Fireworks... p.m. Location: A point on the Connetquot River off Snapper Inn Restaurant, Oakdale, NY in...

  7. Validação de um radioimunoensaio para a quantificação de metabólitos fecais de testosterona em hamster Sírio (Mesocricetus auratus

    Directory of Open Access Journals (Sweden)

    Marie-Odile M Chelini

    2011-05-01

    Full Text Available Modelo para o estudo de diversas doenças humanas, o hamster tem sido objeto de numerosos estudos comportamentais e envolvendo estresse e/ou comportamento agonístico que supõem, muitas vezes, o monitoramento das flutuações hormonais nos indivíduos envolvidos. O objetivo do presente trabalho foi confirmar a adequação de um conjunto comercial para dosagem de testosterona em sangue humano para a quantificação de metabólitos fecais de testosterona (MFT em hamsters Sírios machos e fêmeas. Dez machos foram submetidos a um desafio com um agonista de GnRH para estimular a atividade testicular, elevando os níveis circulantes de testosterona. Cinco fêmeas receberam uma injeção de testosterona enquanto cinco outras receberam uma injeção de solução salina. Amostras de fezes coletadas antes e depois dos procedimentos, assim como amostras fecais de 20 fêmeas gestantes coletadas ao longo da gestação foram analisadas com um conjunto comercial para radioimunoensaio. Um pico de MFT 12h após a injeção seguido de uma queda abaixo do nível basal comprovou que, nos machos, as alterações nos níveis de MFT refletem as alterações da concentração de testosterona no sangue. Nestes observou-se um ciclo circadiano das concentrações de MFT com acrofase no início do período claro correspondendo ao ciclo descrito para as concentrações sanguíneas na literatura. Nas fêmeas a administração de testosterona exógena provocou uma elevação importante dos níveis de MFT, mas as concentrações medidas ao longo da gestação não refletiram o padrão dos níveis sanguíneos do hormônio endógeno. O conjunto para radioimunoensaio para testosterona em sangue humano mostrou-se adequado para o monitoramento da função testicular no hamster macho, mas um ensaio mais específico seria necessário para as fêmeas.

  8. Contaminación aguda por vertidos textiles: Tratamiento de carpines dorados (Carassius auratus) con dicromato potásico; determinación de la toxicidad y niveles de cromo en branquias.

    OpenAIRE

    Riva Juan, María del Carmen; Flos Bassols, Rosa; Crespi Rosell, Martí; Balash, J.

    1980-01-01

    El cromo es el metal pesado cuantitativamente más importante en el vertido de la Industria Textil; procede esencialmente de los procesos de tintura con colorantes cromatables. Hemos sometido carpines dorados al tratamiento agudo con dicromato potásico disuelto en el agua de tanques experimentales. Se determina la toxicidad y los niveles de cromo en branquias. La concentración de cromo en el tejido branquia1 se halla mediante espectrofotometría de absorción atómica, previa digestión c...

  9. SPAWNING IN CONDITION OF THE NORTH-WESTERN PART OF THE MIDDLE CASPIAN OF BLACK SEA ACCLIMATIZED FAMILY MUGILIDAE. TWO SPECIES OF THE GENUS LIZA (L. AURATUS AND (L. SALIENS

    Directory of Open Access Journals (Sweden)

    D. R Adueva

    2012-01-01

    Full Text Available Due to the number of migration changes, adaptive and plastic peculiarities of Mugilidae family two species of grey mullet from Liza family the acclimatization of which in the Caspian Sea formed the shoal of quite valuable marketable fish. Grey mullet sexual puberty in the Caspian Sea happens at the age of 3 or 4. The length and the rate of growth increases. The weight of the Caspian-sea grey mullet is bigger than that of the Black-sea grey mullet.

  10. Absorción, transporte y utilización de los lípidos dietéticos en el intestino e hígado de dorada (sparus auratus) y lubina (dicentrarchus labrax)

    OpenAIRE

    Caballero Cansino, María José

    2002-01-01

    Los lípidos constituyen un componente de gran importancia estructural y funcional en los teleósteos. La elevación de los niveles de lípidos en las dietas comerciales para peces de cultivo tiene un gran interés, ya que permite reducir los niveles de proteína dietética que es el componente más abundante y caro de las dietas para peces. Sin embargo, la limitación en -la producción de aceite de pescado, tradicionalmente la principal fuente de lípidos en las dietas para peces, y el paulatino in...

  11. Estudio sobre tumores melanóticos del jámster sirio. I. Anotaciones sobre una cría pequeña de jámsteres dorados (Mesocricetus auratus

    Directory of Open Access Journals (Sweden)

    José Perea Sasiaín

    1966-10-01

    Full Text Available Para realizar un estudio sobre la inducción de tumores melanóticos por el 9-10 dimetilbenzantraceno, fue necesario iniciar una cría de jamsteres dorados. Estos animales ya habían sido criados con éxito por el doctor Humberto Granados Espitia en la Escuela de Salud Pública de la Universidad Nacional, pero su colonia no pudo ser mantenida un período suficiente de tiempo, y al iniciar mi trabajo no se podían obtener jámsteres adecuados en el país. La cría del jámster dorado se ha efectuado desde 1930 a partir de una hembra capturada con su camada. Desde entonces se demostró que este animal cría bien en cautividad, pero requiere un manejo cuidadoso. Detalles al respecto se pueden encontrar en la excelente tesis de Granados Espitia .

  12. Labeling fish with an activable element through their diet. [samarium

    Energy Technology Data Exchange (ETDEWEB)

    Michibata, Hitoshi (Toyama Univ. (Japan). Faculty of Science)

    1981-10-01

    Stable samarium, one of the rare earth elements, was fed to medaka (Oryzias latipes) and goldfish (Carassius auratus). The concentration of samarium in the labeled fish was determined by neutron activation analysis. In O. latipes, samarium was detectable even 1 yr after the labeled diet was eaten. In C. auratus, samarium was retained in the fifth brachial arch, scales, and gills.

  13. Importation of CyHV-2-infected goldfish into the Netherlands

    NARCIS (Netherlands)

    Ito, Takafumi; Kurita, Jun; Haenen, Olga L.M.

    2017-01-01

    Cyprinid herpesvirus 2 (CyHV-2) is known as the causative agent of herpesviral haematopoietic necrosis in goldfish Carassius auratus auratus. However, the virus has also been detected in Prussian carp C. gibelio and crucian carp C. carassius from European and Asian countries. To prevent spread of

  14. 77 FR 62217 - Gulf of Mexico Fishery Management Council; Public Meetings

    Science.gov (United States)

    2012-10-12

    ... 33607. FOR FURTHER INFORMATION CONTACT: Dr. Stephen Bortone, Executive Director, Gulf of Mexico Fishery... Snapper Benchmark Assessment; review any additional comments from the Scientific and Statistical Committee and from the Socioeconomic Scientific and Statistical Committee; and discuss Exempted Fishing Permits...

  15. Journal of Genetics

    Indian Academy of Sciences (India)

    29

    mangrove red snapper is most valued food fish along the Indo-Pacific region. L. 23 ..... neutrality either due to selection, bottleneck, or population expansion. ..... Based on the above criteria, low nucleotide diversity (π <0.5) and larger haplotype.

  16. Analyses of otoliths from juvenile black sea bass collected from estuaries in SC, FL, NJ, and NY: 2008-2009.

    Data.gov (United States)

    Baruch Institute for Marine and Coastal Sciences, Univ of South Carolina — Snappers/Groupers have traditionally been some of the most desired demersal fishes in the South Atlantic Bight and are the most abundant and diverse group of large...

  17. 77 FR 21955 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Reef Fish Fishery of the Gulf of...

    Science.gov (United States)

    2012-04-12

    ... stock, and that the ABC may be increased. The stock, however, is still overfished and under a rebuilding plan through 2032. The next SEDAR benchmark stock assessment currently scheduled for Gulf red snapper...

  18. 78 FR 32179 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Reef Fish Fishery of the Gulf of...

    Science.gov (United States)

    2013-05-29

    ... snapper stock is still overfished and under a rebuilding plan through 2032. The next SEDAR benchmark stock..., weekend fishing, peak season fishing, increases in stock abundance, potentially significant levels of...

  19. 78 FR 27365 - Gulf of Mexico Fishery Management Council; Public Meeting

    Science.gov (United States)

    2013-05-10

    ... analysts sought advice from the SSC at the March meeting, where it was determined that a proxy based on 30... of explosive oil and gas rig removals on the red snapper stock. Copies of the agenda and other...

  20. The Classroom Animal: Snapping Turtles.

    Science.gov (United States)

    Kramer, David C.

    1987-01-01

    Describes the distinctive features of the common snapping turtle. Discusses facts and misconceptions held about the turtle. Provides guidelines for proper care and treatment of a young snapper in a classroom environment. (ML)

  1. Chrome Tanning Leather of Giant Sea Perch Combined with Seed Extract Areca Nut on the Physical Characteristics

    Directory of Open Access Journals (Sweden)

    Bustami - Ibrahim

    2014-11-01

    Full Text Available Tanning is the process of converting raw hide protein to leather, which are stable, not easily decompose, and is suitable for a variety of uses. The use of vegetable based tanning materials in the leather tanning process has not been carried out. Vegetable based materials that were used are betel nuts. This plant contains tannin which is the main agent in the process of leather tanning. The aim of this study was to determine the physical characteristics of snapper leather treated with betel nut extract. Soxhlet extracting method with methanol as a solvent were used to obtain tannin from betel nuts. Tanned Snapper Leather were analyzed for physical quality, elongation strength, tensile strength, tear strength, and sewing strength. The result showed that methanol extracted betel nut with 10% concentration gives the optimum physical characteristics.Keywords: areca nut, chrome, snapper, snapper

  2. 78 FR 48653 - Gulf of Mexico Fishery Management Council; Public Meeting

    Science.gov (United States)

    2013-08-09

    ...--Recreational Red Snapper Regional Management. 5. Review IFQ Inter-sector Trading Scoping Document. 6. Review... 20-- Modifications to the Coastal Migratory Pelagics Zone Management, and Reef Fish Amendment 39...

  3. 78 FR 49477 - Gulf of Mexico Fishery Management Council; Public Meeting; Correction

    Science.gov (United States)

    2013-08-14

    ... Inter-sector Trading Scoping Document. 6. Review Amendment 28--Red Snapper Allocation Options Paper. 7... Permit Provisions, Mackerel Amendment 20--Modifications to the Coastal Migratory Pelagics Zone Management...

  4. 78 FR 26607 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Reef Fish Fishery of the Gulf of...

    Science.gov (United States)

    2013-05-07

    ... yellowtail snapper. The assessment was reviewed by the Scientific and Statistical Committees (SSCs) of both...: 16 U.S.C. 1801 et seq. 0 2. In Sec. 622.30, paragraph (c) is removed and the introductory paragraph...

  5. Wreckfish Logbook Survey (Vessels)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Federally managed fisheries that currently have mandatory logbook and bycatch reporting requirements for the Southeast Region include snapper/grouper/wreckfish....

  6. EOP Acoustic tagging and monitorings of cultured and wild juvenile crimson jobfish (Pristipomoides filamentosus) in a nursery habitat

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Raw data from Vemco receivers that monitored the Kaneohe, Oahu nursery grounds while tagged juvenile snapper were released in 2006 (cultured) and 2007 (wild). Also...

  7. Resource partitioning among woodpeckers in northeastern Oregon.

    Science.gov (United States)

    Bull Evelyn L.; Steven R. Peterson; Jack Ward. Thomas

    1986-01-01

    Eight species of woodpeckers coexist in conifer forests in northeastern Oregon: northern flicker (Colaptes auratus); yellow-bellied (Sphyrapicus varius) and Williamson's (S. thyroideus) sapsuckers; and pileated (Dryocopus pileatus), hairy (Picoides villosus),...

  8. Toxicity of peracetic acid to fish: Variation among species and impact of water chemistry

    DEFF Research Database (Denmark)

    Straus, David L.; Meinelt, Thomas; Liu, Dibo

    2017-01-01

    , Ictalurus punctatus; golden shiner, Notemigonus crysoleucas; goldfish, Carassius auratus; grass carp, Ctenopharyngodon idella; largemouth bass, Micropterus salmoides; rainbow trout, Oncorhynchus mykiss; sunshine bass, Morone chrysops × M. saxatilis; and walleye, Sander vitreus. Median lethal concentration...

  9. Estudio sobre tumores melanóticos del jámster sirio. II. Efecto de la edad del jámster dorado (Mesocricetus auratus sobre la inducción de tumores melanóticos por el 9,10 dimetilbenzantraceno

    Directory of Open Access Journals (Sweden)

    José Perea Sasiaín

    1966-10-01

    Full Text Available En medicina humana es bien conocida la influencia de la edad sobre la frecuencia del melanoma maligno: este tumor es muy infrecuente, si no excepcional, antes de la pubertad. Los pocos casos documentados de transmisión transplacentaria de tumores, han sido en su mayoría melanomas, lo cual prueba que el infante es susceptible al crecimiento de trasplantes de este tumor, proveniente de la madre. En el jamster dorado se han inducido tumores melanóticos mediante una sola aplicación de 9,10 dimetilbenzantraceno (D MBA. Estos tumores no son considerados malignos por su aspecto histológico, no dar metástasis, ni producir la muerte del animal, ni ser transplantables   regularmente a huéspedes homólogos. Son, sin embargo, de grande importancia para el estudio de la inducción de la melanogénesis. El presente estudio fue realizado "con el fin de establecer la influencia que la edad pudiera tener sobre la cantidad de tumores inducidos por el DMBA. Se consideró en particular que el jámster dorado no alcanza su madurez sexual hasta después del mes (45-60 días, descontando casos excepcionales como el observado a los 28 días.

  10. Estudio sobre tumores melanóticos del jámster sirio. III. Influencia de la castración sobre la inducción de tumores melanóticos por el 9,10 dimetilbenzantraceno en el jámster dorado (Mesocricetus auratus

    Directory of Open Access Journals (Sweden)

    José Perea Sasiaín

    1966-10-01

    Full Text Available En medicina humana el melanoma se observa con mayor frecuencia en la mujer, y los resultados terapéuticos son mejores en ella que en el hombre. Estos resultados han sido atribuidos a mayor atención sobre la piel por razones cosméticas y a un tratamiento más oportuno. En el hombre se han señalado casos excepcionales de influencia gonadal sobre el melanoma, pero la orquedectomia no produce ningún cambio en la evolución de este tumor en el hámster dorado. Procedimos a este estudio para establecer la influencia que puedan tener las gónadas sobre la inducción y desarrollo de las lesiones pigmentarias, pues es conocida la extremada infrecuencia del melanoma antes de la pubertad, en comparación con edades posteriores, así como el hecho de que en 'los jóvenes son frecuentes los nevi de unión, con marcados signos de actividad, sin que lleguen a producir verdaderos melanomas: son los confusamente llamados melanomas juveniles.

  11. The host preference and impact of Argulus japonicus ectoparasite on cyprinids in Central Java, Indonesia

    Science.gov (United States)

    Kismiyati; Wulansari, P. D.; Dewi, N. N.

    2018-04-01

    The most widely cultivated freshwater fish are from Familia Cyprinidae, among others goldfish (Carassius auratus), koi (Cyprinus carpio) and comet goldfish (Carassius auratus auratus). One of the constraints of freshwater fish cultivation is ectoparasite infestation Argulus japonicus. Financial losses have been experienced by some farmers, caused by these ectoparasitic infestaions. This study was aimed to determine the impact of ectoparasite Argulus japonicus infestation on host (freshwater ornamental fish from Familia Cyprinidae), in order to find a preventive solution to treatment on the host. The results showed that prevalence of infested fish by Argulus japonicus were 57 % goldfish, 31 % comet fish and 65 % koi. Changes of histopathology on host were congestion, baoning degeneration, epithelium erosion and inflammatory cell infiltration. The image of infected leukocytes infested by Argulus japonicus were 8.5 % of lymphocytes, 4.7 % neurophils, 3.9 % monocytes, 1.45 % eosinophils and 0,17% basophils.

  12. Metal contents in common edible fish species and evaluation of potential health risks to consumers

    Directory of Open Access Journals (Sweden)

    Naglaa Farag Soliman

    2015-12-01

    Full Text Available Objective: To conduct a health risk assessment of some heavy metals attributed to consumption of common edible fish species available for consumers. Methods: Concentrations of Cd, Cr, Cu, Fe, Mn, Pb and Zn were determined in muscles, gills, livers, bones and skins of six common edible fish species, namely Oreochromis niloticus, Mugil cephalus, Sardinella aurita, Mullus barbatus, Boops boops, Pagrus pagrus. Concentrations of heavy metals were determined by atomic absorption spectrophotometer and expressed as µg/g of wet tissue. Results: Results showed that iron and zinc were the most abundant among all fish tissues under investigation. The data obtained in the present work were compared well with the counterpart data reported internationally. The estimated values of all metals in muscles of fish in this study were below the permissible limits. Moreover, the potential health risks of metals to human via consumption of seafood were assessed by estimating daily intake and target heath quotient. Generally, risk values for the measured metals do not pose unacceptable risks at mean ingestion rate for muscles. Conclusions: It can be concluded that the investigated metals in edible parts of the examined species have no health problems for consumers.

  13. A Study of Some Problems in Network Information Theory

    Science.gov (United States)

    Kamath, Sudeep Uday

    2013-01-01

    Red snapper, "Lutjanus campechanus," were sampled with hook and line at natural (n = 33) and artificial (n = 27) reef sites in the northern Gulf of Mexico from 2009-2011. Stomachs (n = 708) were extracted and their contents preserved for gut content analysis, and muscle tissue samples (n = 200) were dissected and frozen for stable…

  14. 77 FR 64960 - Gulf of Mexico Fishery Management Council; Public Meeting

    Science.gov (United States)

    2012-10-24

    ... webinar and is accessible by computers (Windows or Mac), or by iPhone, iPad, or Android device with the GoToMeeting app available from the App Store or Google Play. A registration link to sign up for the... Southeast Fisheries Science Center will evaluate the effect of this overharvest on the red snapper...

  15. 77 FR 29981 - Fisheries of the Gulf of Mexico; Southeast Data, Assessment, and Review (SEDAR); Public Meetings

    Science.gov (United States)

    2012-05-21

    ... describes the fisheries, evaluates the status of the stock, estimates biological benchmarks, projects future... population models to evaluate stock status, estimate population benchmarks and Magnuson-Stevens... assessment of the Gulf of Mexico stock of red snapper will consist of a series of three workshops: a Data...

  16. 78 FR 20292 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Reef Fish Fishery of the Gulf of...

    Science.gov (United States)

    2013-04-04

    ... for the red snapper stock, and that the acceptable biological catch (ABC) may be increased. The stock, however, is still overfished and is under a rebuilding plan through 2032. The next SEDAR benchmark stock... stock abundance, potentially significant levels of deliberate or accidental non-compliance by...

  17. Molecular authentication of Pargo fillets Lutjanus purpureus (Perciformes: Lutjanidae by DNA barcoding reveals commercial fraud

    Directory of Open Access Journals (Sweden)

    Ivana Veneza

    2018-03-01

    Full Text Available ABSTRACT The Caribbean Red Snapper (Pargo Lutjanus purpureus is the most economically important snapper in Brazil, which is sold, among other forms, as frozen fillets. During the process of transformation into fillets there is the removal of the distinctive morphological traits, being able to favor the substitution by less valued species. In addition, there is no national legislation requiring the insertion of the specific name on the product label. However, according to a Normative Instruction (IN N ° 29/2015 MAPA that correlates the common and specific names of the products destined to the national trade, in Brazil only L. purpureus and L. campechanus can be denominated “Pargo”. Thus, the DNA barcode tool was used to identify the fillets sold in north of Brazil, labeled “Pargo”, with the aid of sequences from the public and control databases. The results showed that among 142 fillets examined, 78% was identified as L. purpureus and 22% as Rhomboplites aurorubens, a snapper with low commercial value in the country, revealing commercial fraud. The molecular identification method successfully used in this study to authenticate fillets snappers may also be used by surveillance authorities in the quality control of processed fish products, towards ensuring consumer rights.

  18. Destructive fishing and fisheries enforcement in eastern Indonesia

    NARCIS (Netherlands)

    Bailey, M.L.; Sumaila, U.R.

    2015-01-01

    A simple bioeconomic leader-follower model was constructed to simulate snapper (family Lutjanidae) and grouper (family Serranidae) fisheries in Raja Ampat, Indonesia, an area of significant coral and fish biodiversity. We developed a leader-follower game, wherein the Regency government as the leader

  19. Spatial and Temporal Distribution of Reef Fish Spawning ...

    African Journals Online (AJOL)

    The spatial patterns among fish families were attributed to a combination of differences in species abundance and distribution as well as variation in fishing effort. Spawning periodicity reported by fishers indicated that for snappers and rabbitfishes, the most activity occurred across a protracted period of October to April/May, ...

  20. 50 CFR Appendix A to Part 635 - Species Tables

    Science.gov (United States)

    2010-10-01

    ... Lemon, Negaprion brevirostris Nurse, Ginglymostoma cirratum Scalloped hammerhead, Sphyrna lewini Smooth..., Lutjanus analis Nurse shark, Ginglymostoma cirratum Queen snapper, Etelis oculatus Red grouper, Epinephelus... and will not become effective until approval has been given by the Office of Management and Budget. ...

  1. 78 FR 17882 - Recreational Closure Authority Specific to Federal Waters Off Individual States for the...

    Science.gov (United States)

    2013-03-25

    ... support of this emergency rule, which include an environmental assessment, may be obtained from the... Louisiana and Florida have recently indicated they will implement inconsistent recreational red snapper... reductions would be needed if other Gulf states (Mississippi and Alabama) also implement inconsistent...

  2. 75 FR 24883 - Fisheries of the South Atlantic; Southeast Data, Assessment, and Review (SEDAR); Public Meeting

    Science.gov (United States)

    2010-05-06

    ... Assessment Process is a stock assessment report which describes the fisheries, evaluates the status of the stock, estimates biological benchmarks, projects future population conditions, and recommends research... South Atlantic stock of red snapper will consist of a series of workshops and webinars: a Data Workshop...

  3. Response Acquisition under Targeted Percentile Schedules: A continuing Quandary for Molar Models of Operant Behavior

    Science.gov (United States)

    1993-07-01

    dimensions topics such as timing (e.g., Gibbon & Allan, from interresponse-time duration (e.g.. Alle- 1984), numerositv (e.g., Gallistel , 1989), and...Behavior, 51, 145-162. Snapper, A. G., & Inglis, G. B. (1985). SKED-li soft- Gallistel , C. R. (1989). Animal cognition: The repre- ware system. Kalamazoo

  4. Ciguatera: the detection of neurotoxins in carnivorous reef fish from ...

    African Journals Online (AJOL)

    Two barracuda and one snapper tested positive for a sodium-channel activator, i.e. presumptive ciguatoxin, in the N2a assay. LC/MS analyses showed that only these three samples contained high-intensity peaks, with masses of 1 222 amu and 1 279 amu. These results represent the first analytical report indicating the ...

  5. 78 FR 49440 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Reef Fish Fishery of the Gulf of...

    Science.gov (United States)

    2013-08-14

    ... Regulatory Flexibility Act analysis may be obtained from the Southeast Regional Office Web site at http... (SEDAR 31), conducted in 2013, determined that the acceptable biological catch (ABC) for red snapper... May 2013 to review SEDAR 31, and recommended an ABC of 13.5 million lb (6.1 million kg), round weight...

  6. 77 FR 56168 - Reef Fish Fishery of the Gulf of Mexico; Gulf of Mexico Individual Fishing Quota Programs

    Science.gov (United States)

    2012-09-12

    .... 090206140-91081-03] RIN 0648-XC227 Reef Fish Fishery of the Gulf of Mexico; Gulf of Mexico Individual... red snapper and grouper/tilefish components of the reef fish fishery in the Gulf of Mexico (Gulf), the... INFORMATION: The reef fish fishery of the Gulf of Mexico is managed under the Fishery Management Plan for Reef...

  7. Mitochondrial signatures revealed panmixia in Lutjanus ...

    Indian Academy of Sciences (India)

    user

    mangrove red snapper is most valued food fish along the Indo-Pacific region. ... conservation of genetic diversity of our natural resources is beneficial in genetic ... to be fast evolving (1.3% per million years) and used to detect the population ...

  8. 76 FR 82183 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Comprehensive Ecosystem-Based...

    Science.gov (United States)

    2011-12-30

    ... THE CARIBBEAN, GULF, AND SOUTH ATLANTIC 0 1. The authority citation for part 622 continues to read as... appropriate to secure the range of hook sizes and styles used in the South Atlantic snapper- grouper fishery.... The device must be of a size appropriate to secure the range of hook sizes and styles used in the...

  9. 78 FR 20277 - Safety Zones & Special Local Regulations; Recurring Marine Events in Captain of the Port Long...

    Science.gov (United States)

    2013-04-04

    .... Regulatory History and Information The Coast Guard promulgated safety zones and special local regulations for... first week of July. Location: Waters of the Connetquot River off Snapper Inn Restaurant, Oakdale, NY in...: Waters of Patchogue Bay off ``Lombardi's On the Bay'' restaurant, Patchogue, NY in approximate position...

  10. 76 FR 3616 - Gulf of Mexico Fishery Management Council; Public Meetings

    Science.gov (United States)

    2011-01-20

    ...; Outreach & Education; Coral; Spiny Lobster/Stone Crab; and Coastal Migratory Pelagic (Mackerel). 5:15 p.m... red grouper stock assessment; review an options paper for a red snapper regulatory amendment to adjust... individual fishing quota discussion paper. Recess Immediately Following Committee Recess--There will be an...

  11. Louisiana Coastal Area, Louisiana. Land Loss and Marsh Creation

    Science.gov (United States)

    1984-11-01

    Blue runner Southern flounder Crevalle jack American oyster Greater amberjack Rangia clam Florida pompano White shrimp Dolphin Brown shrimp Red snapper...are winter residents and the Mississippi kite and broad-winged hawk are common summer residents (Lowery 1974a). -7. 0 Oftmm# za oSZ I.- ~ ~ M j cz~~e

  12. 76 FR 50979 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Reef Fish Fishery of the Gulf of...

    Science.gov (United States)

    2011-08-17

    ... tackle shops, and hotels and motels, in addition to environmental groups, student clubs, and other groups...- information displays a currently valid Office of Management and Budget (OMB) control number. This proposed... transaction; weight and actual ex-vessel price of red snapper landed and sold; and information necessary to...

  13. 78 FR 31402 - Safety Zones and Special Local Regulations; Recurring Marine Events in Captain of the Port Long...

    Science.gov (United States)

    2013-05-24

    ... Operations, telephone (202) 366-9826. SUPPLEMENTARY INFORMATION: Table of Acronyms COTP Captain of the Port... River off Snapper Inn Restaurant, Oakdale, NY in approximate position 40[deg]43'32.38'' N, 073[deg]9'02...'' restaurant Patchogue, NY in approximate position 40[deg]44'39.18'' N 073[deg]00'37.80'' W (NAD 83). 11.3...

  14. Recent foraminiferal assemblages from the continental shelf sediments of Madras, Bay of Bengal

    Digital Repository Service at National Institute of Oceanography (India)

    Setty, M.G.A.P.

    During the 15th cruise of INS Kistna, as part of the International Indian Ocean Expedition, several sub-surface samples were collected, (using LaFond-Dietz snapper), from the continental shelf region of the Bay of Bengal. Samples from two locations...

  15. 78 FR 56217 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Shrimp Fisheries of the Gulf of...

    Science.gov (United States)

    2013-09-12

    ... Atlantic States AGENCY: National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric... specified in 50 CFR part 622, Subparts A through R for reef fish, red drum, coastal migratory pelagics, and spiny lobster in the Gulf of Mexico, and snapper-grouper, coastal migratory pelagics, dolphin and wahoo...

  16. 76 FR 35409 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Shrimp Fisheries of the Gulf of...

    Science.gov (United States)

    2011-06-17

    ... Atlantic States AGENCY: National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric..., red drum, coastal migratory pelagics, stone crab, and lobsters in the Gulf, and snapper-grouper, coastal migratory pelagics, dolphin and wahoo, and lobsters in the South Atlantic. The EFP exempts...

  17. Potential of Pigeon Creek, San Salvador, Bahamas, as Nursery Habitat for Juvenile Reef Fish

    Directory of Open Access Journals (Sweden)

    Conboy, Ian Christopher

    2011-10-01

    Full Text Available This project assessed the significance of Pigeon Creek, San Salvador, Bahamas as a nursery habitat for coral reef fishes. Pigeon Creek’s perimeter is lined with mangrove and limestone bedrock. The bottom is sand or seagrass and ranges in depth from exposed at low tide to a 3-m deep, tide-scoured channel. In June 2006 and January 2007, fish were counted and their maturity was recorded while sampling 112 of 309 possible 50-m transects along the perimeter of the Pigeon Creek. Excluding silversides (Atherinidae, 52% of fish counted, six families each comprised >1% of the total abundance (Scaridae/parrotfishes, 35.3%; Lutjanidae/snappers, 23.9%; Haemulidae/grunts, 21.0%; Gerreidae/mojarras, 8.5%; Pomacentridae/damselfishes, 6.1%; Labridae/wrasses, 2.4%. There were few differences in effort-adjusted counts among habitats (mangrove, bedrock, mixed, sections (north, middle, southwest and seasons (summer 2006 and winter 2007. Red Mangrove (Rhizophora mangle, covering 68% of the perimeter was where 62% of the fish were counted. Snappers, grunts and parrotfishes are important food fishes and significant families in terms of reef ecology around San Salvador. Mangrove was the most important habitat for snappers and grunts; bedrock was most important for parrotfishes. The southwest section was important for snappers, grunts and parrotfishes, the north section for grunts and parrotfishes, and the middle section for snappers. Among the non-silverside fish counted, 91.2% were juveniles. These results suggest that Pigeon Creek is an important nursery for the coral reefs surrounding San Salvador and should be protected from potential disturbances.

  18. Evolutionary history of the somatostatin and somatostatin receptors

    Indian Academy of Sciences (India)

    Somatostatin and its receptors have a critical role in mammalian growth through their control pattern of secretion of growth hormone, but the evolutionary history of somatostatin and somatostatin receptors are ill defined. We used comparative whole genome analysis of Danio rerio, Carassius auratus, Xenopus tropicalis, ...

  19. Effect of storage media and time on fin explants culture in the ...

    African Journals Online (AJOL)

    The effect of storage media and time was investigated on fin explants culture in the goldfish (Carassius auratus). Fin explants under sterile conditions were able to produce cells at different storage media and time. On the outgrowth of cells, fin explants stored for seven days before culturing showed significantly higher growth ...

  20. Temporal changes in cranial size in South African vlei rats ( Otomys ...

    African Journals Online (AJOL)

    We used the greatest length of the skull (GLS) as a robust indicator of body size. Cranial size of both O. auratus and O. angoniensis decreased significantly over the past 100 years, as expected given historical evidence of warming across northern South Africa since the 1950s. In terms of geographical patterns, GLS was ...

  1. Development of Chronic and Acute Golden Syrian Hamster Infection Models with Leptospira borgpetersenii serovar Hardjo

    Science.gov (United States)

    The golden Syrian hamster (Mesocricetus auratus) is frequently used as a model to study virulence for several species of Leptospira. Onset of an acute, lethal infection following infection with several pathogenic Leptospira species has been widely adopted for vaccine testing. An important exceptio...

  2. Differences in iron concentration in whole blood of animal models using NAA

    International Nuclear Information System (INIS)

    Bahovschi, V; Zamboni, C B; Silva, L F F Lopes; Metairon, S; Medeiros, I M M A

    2015-01-01

    In this study Neutron Activation Analysis technique (NAA) was applied to determine Fe concentration in whole blood samples of several animal models such as: mice (Mus musculus), Golden Hamster (Mesocricetus auratus), Wistar rats, Albinic Rabbits of New Zealand, Golden Retriever dogs and Crioulabreed horses. These results were compared with human whole blood estimation to check their similarities. (paper)

  3. Differences in iron concentration in whole blood of animal models using NAA

    Science.gov (United States)

    Bahovschi, V.; Zamboni, C. B.; Lopes Silva, L. F. F.; Metairon, S.; Medeiros, I. M. M. A.

    2015-07-01

    In this study Neutron Activation Analysis technique (NAA) was applied to determine Fe concentration in whole blood samples of several animal models such as: mice (Mus musculus), Golden Hamster (Mesocricetus auratus), Wistar rats, Albinic Rabbits of New Zealand, Golden Retriever dogs and Crioulabreed horses. These results were compared with human whole blood estimation to check their similarities.

  4. Metabolic rate changes proportionally to circadian frequency in tau mutant Syrian hamsters

    NARCIS (Netherlands)

    Oklejewicz, M; Hut, RA; Daan, S; Loudon, ASI; Stirland, AJ; Loudon, Andrew S.I.; Stirland, Anne J.

    1997-01-01

    The tau mutation in Syrian hamsters (Mesocricetus auratus) is phenotypically expressed in a period of the circadian rhythm of about 20 h in homozygotes (SS) and about 22 h in heterozygotes (S+). The authors investigate whether this well-defined model for variation in circadian period exhibits

  5. African Journal of Health Sciences - Vol 14, No 3 (2007)

    African Journals Online (AJOL)

    ... study of Multiple versus Single infection doses of Schistosoma haematobium in Golden hamsters (Mesocricetus auratus) · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. VK Njoroge, F Nyundo, M Limo, DS Yole, 187-194. http://dx.doi.org/10.4314/ajhs.v14i3.30864 ...

  6. Vasopressin immunoreactivity and release in the suprachiasmatic nucleus of wild-type and tau mutant Syrian hamsters

    NARCIS (Netherlands)

    Van der Zee, EA; Oklejewicz, M; Jansen, K; Daan, S; Gerkema, MP

    2002-01-01

    Despite the prominent role of the Syrian hamster (Mesocricetus auratus) in studies of circadian rhythms, there are no data available on the temporal dynamics of the neuropeptide vasopressin (AVP), a major output system of the suprachiasmatic nucleus (SCN). We studied the hamster SCN-AVP system in

  7. Download this PDF file

    African Journals Online (AJOL)

    Administrator

    The sex-ratio of most fish in the lake tends to unity (1:1), suggesting reproductive stability. Condition factors are high for the fish species ... Parachanna obscuraGunther. Snake head. Characidae. Chrysichthys auratusPfaff. Characines. Cichlidae. Hemichromis faciatusPeter. Banded jewel fish. Oreochromis niloticusLinnaeus.

  8. Expertomica Fishgui: comparison of fish skin colour

    Czech Academy of Sciences Publication Activity Database

    Urban, J.; Štys, D.; Sergejevova, Magda; Masojídek, Jiří

    2013-01-01

    Roč. 29, č. 1 (2013), s. 172-180 ISSN 0175-8659 R&D Projects: GA ČR GA521/09/0656 Institutional support: RVO:61388971 Keywords : CARASSIUS-AURATUS * IMAGE-ANALYSIS * DIETARY SUPPLEMENTATION Subject RIV: EE - Microbiology, Virology Impact factor: 0.903, year: 2013

  9. Food habits of Northern Goshawks nesting in south central Wyoming

    Science.gov (United States)

    John R. Squires

    2000-01-01

    Northern Goshawks (Accipiter gentiles) nesting in south central Wyoming consumed at least 33 species of prey; 14 were mammals and 19 were birds. Based on percent occurrence in regurgitated pellets, dominant (>10% frequency) prey species included: red squirrel (Tamiasciurus hudsonicus; present in 50% of pellets), Northern Flicker (Colaptes auratus; 34...

  10. Environmental Assessment for the Construction and Operation of an Indoor Small Arms Range at Niagara Falls Air Reserve Station, New York

    Science.gov (United States)

    2009-08-01

    Name Northern flicker Colaptes auratus Northern harrier2 Circus cyaneus Northern mockingbird Mimus polyglottos Northern rough-winged swallow...Installation operations impact wildlife habitat. Disturbance of vegetation by construction. Displacement and potential to kill or injure...Unless otherwise permitted by regulations, the MBTA makes it unlawful to pursue, hunt, take, capture, or kill ; attempt to take, capture or kill

  11. Studies of FAUNA at Eglin Air Force Base

    Science.gov (United States)

    2006-07-01

    occasionally resulted in fires that were hot enough to kill mature pines, including the old growth pines upon which RCWs depend for cavity excavation...cardinalis NOCA Northern flicker* Colaptes auratus NOFL Northern mockingbird Mimus polyglottos NOMO Northern rough-winged swallow Stelgidopteryx

  12. Phylogeny and biogeographic history of the cyprinid fish genus Carassius (Teleostei: Cyprinidae) with focus on natural and anthropogenic arrivals in Europe

    Czech Academy of Sciences Publication Activity Database

    Rylková, K.; Kalous, L.; Bohlen, Jörg; Lamatsch, D.K.; Petrtýl, M.

    2013-01-01

    Roč. 380, č. 383 (2013), s. 13-20 ISSN 0044-8486 R&D Projects: GA ČR GA206/09/1154 Institutional research plan: CEZ:AV0Z5045916 Institutional support: RVO:67985904 Keywords : Carassius auratus complex * Cytochrome b * Distribution Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.828, year: 2013

  13. The influence of low-level radiation on nucleolus activity of cyprinidae embryos

    International Nuclear Information System (INIS)

    Arkhipchuk, V.V.

    1990-01-01

    The number and sizes of nucleoli in embryos of Cyprinus carpio and Carassius auratus gibelio exposed to low-level radiation at a dose-rate of 10 μ Gy/h and down were larger than those on non-irradiated controls

  14. Is humane slaughter of fish possible for industry?

    NARCIS (Netherlands)

    Vis, van de J.W.; Kestin, S.; Robb, D.; Oehlenschlaeger, J.; Lambooij, E.; Kuhlmann, H.; Kloosterboer, K.

    2003-01-01

    The objective was to evaluate industrial and research slaughter methods for Atlantic salmon (Salmo salar), gilt-head seabream (Sparus auratus) and eel (Anguilla anguilla) with respect to welfare and quality. As a general term of reference, an optimal slaughter method should render fish unconscious

  15. Finding of No Significant Impact & Tiered Environmental Assessment: Public Law 84-99 Rehabilitation Program Levee Unit 624-627 - Mosquito Creek Pottawattamie County, Iowa

    Science.gov (United States)

    2014-10-01

    spp.), northern flicker (Colaptes auratus), red-headed woodpecker (Melanerpes erythrocephalus), wild turkey (Meleagris gallopavo), eastern kingbird...aquatic environment. Some commenters asked that compensatory mitigation be required for all activities authorized by this NWP. A few commenters...remarked that compensatory mitigation should be required for adverse effects on high quality riparian areas. Another commenter said that mitigation should

  16. Identification and characterisation of an androgen receptor from zebrafish Danio rerio

    DEFF Research Database (Denmark)

    Jørgensen, Anne; Andersen, Ole; Bjerregaard, Poul

    2007-01-01

    ) and goldfish (Carassius auratus). Binding assays with zfAR demonstrated high affinity, saturable, single class binding site, with the characteristics of an androgen receptor. Saturation experiments along with subsequent Scatchard analysis determined that the Kd of the zfAR for 3H-testosterone was 2 n...

  17. Trichodina nobilis Chen, 1963 and Trichodina reticulata Hirschmann et Partsch, 1955 from ornamental freshwater fishes in Brazil

    Directory of Open Access Journals (Sweden)

    ML Martins

    Full Text Available In the present work Trichodina reticulata and T. nobilis (Ciliophora: Trichodinidae are morphologically characterised from ornamental freshwater fish culture in the State of Santa Catarina, Brazil. The prevalence of infection and a list of comparative measurements are discussed. We examined "southern platyfish" Xiphophorus maculatus (n = 35, "goldfish" Carassius auratus (n = 31, "guppy" Poecilia reticulata (n = 20, "sailfin molly" Poecilia latipinna (n = 6, "beta" Betta splendens (n = 2 and "spotted headstander" Chilodus punctatus (n = 1. After being anesthetised in a benzocaine solution, fishes were examined for parasitological evaluation. A total of 51.57% fishes were parasitised by Trichodina spp. Carassius auratus was the most parasitised species, followed by X. maculatus and P. reticulata. Beta splendens, C. punctatus and P. latipinna were not parasitised by any trichodinid species. Two species of Trichodina were collected from the skin of fish: T. nobilis was found in C. auratus, P. reticulata and X. maculatus and T. reticulata was only observed in C. auratus. The importance of adequate handling in ornamental fish culture are also discussed.

  18. Host use evolution in Chrysochus milkweed beetles: evidence from behaviour, population genetics and phylogeny.

    Science.gov (United States)

    Dobler, S; Farrell, B D

    1999-08-01

    In two sister species of leaf beetles with overlapping host associations, Chrysochus auratus and C. cobaltinus, we established diet breadth and food preference of local populations for evaluation together with genetic differentiation between populations. While C. auratus turned out to be monophagous on the same plant wherever we collected the beetles, the studied populations of C. cobaltinus fed on three different plant species in the field. Plant preference and ranking of the potential host plants significantly differed between these populations. The amount of genetic differentiation between populations was measured by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay of a 1300 bp mitochondrial DNA (mtDNA) sequence. In addition, the dominant genotypes of all populations were sequenced. No genetic differentiation between the populations of C. auratus could be detected in the RFLP assay and sequence divergence was low (= 0.3%). In C. cobaltinus, on the other hand, genetic differentiation between populations was high, revealing a lack of gene flow over a much smaller scale and a maximum of 1.3% sequence divergence. C. cobaltinus thereby has the prerequisites for host race formation on different plants from the original host spectrum. Our sequence-based phylogeny estimate allows us to reconstruct historical diet evolution in Chrysochus. Starting from an original association with Asclepiadaceae, the common ancestor of C. auratus and C. cobaltinus included Apocynaceae in its diet. The strict specialization on Apocynum and the loss of acceptance of Asclepiadaceae observed in C. auratus could have resulted from a process similar to that displayed by C. cobaltinus populations.

  19. Biochemical characteristics of four marine fish skins in Korea.

    Science.gov (United States)

    Cho, Jae-Kwon; Jin, Young-Guk; Rha, Sung-Ju; Kim, Seon-Jae; Hwang, Jae-Ho

    2014-09-15

    In this study, we investigated the biochemical characteristics of the fish skins of four industrial species: olive flounder (Paralichthys olivaceus), black rockfish (Sebastes schlegeli), sea bass (Lateolabrax maculatus) and red sea bream (Pagrus major). There is high domestic demand in Korea for farming of these fish for human consumption. Crude protein contents in the skin of these fish ranged from 73% to 94% by dry weight; this was in part due to a high content of the structural protein, collagen. Among the four species, olive flounder had the thickest dermal and epidermal layers in the dorsal skin. This species was also associated with the highest extraction ratio of acid-soluble collagen. We also examined whether fish skin could be a cost-effective alternative to current fish meal sources. Our analysis indicates that, when supplemented with additional fish oils and essential amino acids, fish skin is a viable alternative for fish meal formulations. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. Behavioral Response of Reef Fish and Green Sea Turtles to Midfrequency Sonar.

    Science.gov (United States)

    Watwood, Stephanie L; Iafrate, Joseph D; Reyier, Eric A; Redfoot, William E

    2016-01-01

    There is growing concern over the potential effects of high-intensity sonar on wild fish populations and commercial fisheries. Acoustic telemetry was employed to measure the movements of free-ranging reef fish and sea turtles in Port Canaveral, FL, in response to routine submarine sonar testing. Twenty-five sheepshead (Archosargus probatocephalus), 28 gray snapper (Lutjanus griseus), and 29 green sea turtles (Chelonia mydas) were tagged, with movements monitored for a period of up to 4 months using an array of passive acoustic receivers. Baseline residency was examined for fish and sea turtles before, during, and after the test event. No mortality of tagged fish or sea turtles was evident from the sonar test event. There was a significant increase in the daily residency index for both sheepshead and gray snapper at the testing wharf subsequent to the event. No broad-scale movement from the study site was observed during or immediately after the test.

  1. Decision Making and Training: A Review of Theoretical and Empirical Studies of Decision Making and Their Implications for the Training of Decision Makers

    Science.gov (United States)

    1975-08-01

    13-C-0128-] Pruitt, 1961; Schrenk, 1964; Snapper & Peterson, 1971; Swots & Birdsall, 1967). Thc results of most of these studies suggest that although...numerous attempts to apply decision trees and flow diaqrams to the solution of decision problems (e.g., Baker, 1967; Clarkson, 1963; Dutton & Starbuck ...Baltimore: Penguin Books, 1968, 28-43. i 192 6• NAVTRAEQUTPCEN 73-C-0128-1 Dutton, J. M. & Starbuck , W. H. Finding Charlie’s run time estimator. In J

  2. Po-210 and Pb-210 in water and fish from Taboshar uranium mining Pit Lake, Tajikistan

    International Nuclear Information System (INIS)

    Skipperud, L.; Jørgensen, A.G.; Heier, L.S.; Salbu, B.; Rosseland, B.O.

    2013-01-01

    Polonium-210 in water and 210 Pb and 210 Po in different fish organs from 3 different fish species in Taboshar Pit Lake (n = 13), located in the uranium mining area in Tajikistan, and in Kairakkum Reservoir (reference lake, n = 3), have been determined as part of a Joint project between Norway, Kazakhstan, Kyrgyzstan and Tajikistan. The average activity concentration of 210 Pb and 210 Po in liver, muscle and bone of Carassius auratus was higher than the concentration in similar tissues of C. carpio and Sander lucioperca from the reference site. The accumulation of 210 Po was higher than for 210 Pb, and the accumulation of 210 Po was highest in the liver of C. auratus (3673 ± 434 Bq kg −1 ww). Although the average activity concentration of 210 Pb in liver and bones of C. auratus from Pit Lake were fairly similar, a huge variation in the liver activity concentrations (25–327 Bq kg −1 ww) was found. The results confirm direct uptake of unsupported 210 Po into the liver, and that the distributions of 210 Po and 210 Pb in fish organs were different. The BCF (L/kg) for 210 Po in bone, liver and muscle clearly demonstrates high accumulation of 210 Po in C. auratus, especially in the liver. The average BCFs of liver, bone and muscle were >1.4 × 10 5 , >2.5 × 10 4 and >1.4 × 10 4 , respectively. All fish in the Pit Lake were found to be in the same trophic level, however, a linear correlation between log 210 Po in liver and δ 15 N could indicate biomagnification of 210 Po in liver of C. auratus. In regards to the recommended Annual Limit of Intake (ALI) for 210 Po, the concentration of 210 Po in muscle tissues of C. auratus is alarming, as there is a high probability for the local population at risk to exceed the recommended ALI through consumption of fish from Taboshar Pit Lake

  3. Feeding ecology of Lutjanus analis (Teleostei: Lutjanidae from Abrolhos Bank, Eastern Brazil

    Directory of Open Access Journals (Sweden)

    Matheus Oliveira Freitas

    Full Text Available Diet and feeding ecology of the mutton snapper Lutjanus analis were investigated in the Abrolhos Bank, Eastern Brazil, the largest and richest coral reefs in the South Atlantic, where about 270 species of reef and shore fishes occur. To evaluate seasonal and ontogenetic shifts in the diet, specimens of L. analis were obtained through a fish monitoring program in four cities in southern Bahia State, from June 2005 to March 2007. Stomachs from 85 mutton snappers that ranged in size from 18.1 to 74.0 cm TL were examined. Prey were identified to the lowest possible taxon and assessed by the frequency of occurrence and volumetric methods. Variations in volume prey consumption were evaluated using non-metric multi-dimensional scaling ordination, analysis of similarity, and similarity percentage methods. Significant differences in diet composition among size classes were registered, whereas non significant differences between seasons were observed. Considering size-classes, food items consumption showed important variations: juveniles (50.1 cm TL consumed basically fish, mostly Anguiliformes. Lutjanus analis is an important generalist reef predator, with a broad array of food resources and ontogenetic changes in the diet. This snapper species plays an important role on the trophic ecology of the Abrolhos Bank coral reefs.

  4. Comparing relative abundance, lengths, and habitat of temperate reef fishes using simultaneous underwater visual census, video, and trap sampling

    KAUST Repository

    Bacheler, NM

    2017-04-28

    Unbiased counts of individuals or species are often impossible given the prevalence of cryptic or mobile species. We used 77 simultaneous multi-gear deployments to make inferences about relative abundance, diversity, length composition, and habitat of the reef fish community along the southeastern US Atlantic coast. In total, 117 taxa were observed by underwater visual census (UVC), stationary video, and chevron fish traps, with more taxa being observed by UVC (100) than video (82) or traps (20). Frequency of occurrence of focal species was similar among all sampling approaches for tomtate Haemulon aurolineatum and black sea bass Centropristis striata, higher for UVC and video compared to traps for red snapper Lutjanus campechanus, vermilion snapper Rhomboplites aurorubens, and gray triggerfish Balistes capriscus, and higher for UVC compared to video or traps for gray snapper L. griseus and lionfish Pterois spp. For 6 of 7 focal species, correlations of relative abundance among gears were strongest between UVC and video, but there was substantial variability among species. The number of recorded species between UVC and video was correlated (ρ = 0.59), but relationships between traps and the other 2 methods were weaker. Lengths of fish visually estimated by UVC were similar to lengths of fish caught in traps, as were habitat characterizations from UVC and video. No gear provided a complete census for any species in our study, suggesting that analytical methods accounting for imperfect detection are necessary to make unbiased inferences about fish abundance.

  5. Predator effects on reef fish settlement depend on predator origin and recruit density.

    Science.gov (United States)

    Benkwitt, Cassandra E

    2017-04-01

    During major life-history transitions, animals often experience high mortality rates due to predation, making predator avoidance particularly advantageous during these times. There is mixed evidence from a limited number of studies, however, regarding how predator presence influences settlement of coral-reef fishes and it is unknown how other potentially mediating factors, including predator origin (native vs. nonnative) or interactions among conspecific recruits, mediate the non-consumptive effects of predators on reef fish settlement. During a field experiment in the Caribbean, approximately 52% fewer mahogany snapper (Lutjanus mahogoni) recruited to reefs with a native predator (graysby grouper, Cephalopholis cruentata) than to predator-free control reefs and reefs with an invasive predator (red lionfish, Pterois volitans) regardless of predator diet. These results suggest that snapper recruits do not recognize nonnative lionfish as a threat. However, these effects depended on the density of conspecific recruits, with evidence that competition may limit the response of snapper to even native predators at the highest recruit densities. In contrast, there was no effect of predator presence or conspecific density on the recruitment of bicolor damselfish (Stegastes partitus). These context-dependent responses of coral-reef fishes to predators during settlement may influence individual survival and shape subsequent population and community dynamics. © 2017 by the Ecological Society of America.

  6. Attempt at cloning high-quality goldfish breed 'Ranchu' by fin-cultured cell nuclear transplantation.

    Science.gov (United States)

    Tanaka, Daisuke; Takahashi, Akito; Takai, Akinori; Ohta, Hiromi; Ueno, Koichi

    2012-02-01

    The viability of ornamental fish culture relies on the maintenance of high-quality breeds. To improve the profitability of culture operations we attempted to produce cloned fish from the somatic nucleus of the high-quality Japanese goldfish (Carassius auratus auratus) breed 'Ranchu'. We transplanted the nucleus of a cultured fin-cell from an adult Ranchu into the non-enucleated egg of the original goldfish breed 'Wakin'. Of the 2323 eggs we treated, 802 underwent cleavage, 321 reached the blastula stage, and 51 reached the gastrula stage. Two of the gastrulas developed until the hatching stage. A considerable number of nuclear transplants retained only the donor nucleus. Some of these had only a 2n nucleus derived from the same donor fish. Our results provide insights into the process of somatic cell nuclear transplantation in teleosts, and the cloning of Ranchu.

  7. Strontium-90 and cesium-137 in freshwater fish

    International Nuclear Information System (INIS)

    1983-01-01

    Sr-90 and Cs-137 in freshwater fish were determined using radiochemical analysis. Three species of fish (Carassius auratus, Cyprinus carpio, and Hypomesus transpacificus nipponensis) were collected during the fishing season from seven sampling locations. Only edible part was used in case of larger sized fish, and the whole part was used in case of smaller ones. Each sample was weighed and placed in a stainless steel pan or a porcelain dish. After carbonized, the sample was ashed in an electric muffle furnace. The maximum values of Sr-90 and Cs-137 were 49.0 +- 1.30 pCi/kg and 8.5 +- 0.64 pCi/kg in Carassius auratus collected from Kyoto and Fukui, respectively, in December 1983. (Namekawa, K.)

  8. Strontium-90 and cesium-137 in freshwater fish (from Jul. 1984 to Dec. 1984)

    International Nuclear Information System (INIS)

    1984-01-01

    Sr-90 and Cs-137 in freshwater fish were determined using radiochemical analysis. Three species of fish (Cyprinus carpio, Carassius auratus, and Hypomesus transpacificus nipponensis) were collected during the fishing season from eight sampling locations. Only the edible part was used in case of larger sized fish, and the whole part was used in case of smaller ones. Each sample was weighed and placed in a stainless steel pan or a porcelain dish. After carbonized, the sample was ashed in an electric muffle furnace. The maximum value of Sr-90 was 96 +- 1.7 pCi/kg in Cyprinus carpio collected from Akita in July 1984; and that of Cs-137 was 11 +- 0.7 pCi/kg in Carassius auratus from Fukui in December 1984. (Namekawa, K.)

  9. Scale brightness of common goldfish on diet supplemented with seaweed Sargassum sp. and pumpkin Cucurbita moschata

    Directory of Open Access Journals (Sweden)

    Irfina Manda Astari

    2016-02-01

    Full Text Available ABSTRACT The common goldfish Carassius auratus auratus is an ornamental freshwater fish species with  high market value. The market value of ornamental fish can be determined through some parameters, one of them is brightness of the fish scales. Brightness level may be enhanced by supplementing plant powder containing carotenoid into fish diet. The purpose of this research was to examine the scale brightness level of common goldfish fed diet supplemented with Sargassum sp. and pumpkin powder, and to determine the dose of those powders that allows higher brightness level of scale.  The feed of common goldfish was supplemented with either Sargassum sp. or pumpkin powder at the doses of 10, 20 and 30%, and without supplementation as control. Fishes were reared for 75 days and fed diet at feeding rate of 5% biomass daily. The results of ANOVA test showed that the Sargassum sp. and pumpkin mill addition on food significantly improved of scales brightness of  common goldfish. Increasing supplementation levels of Sargassum sp. and pumpkin powder increased brightness level of scale. The highest improvement in brightness was the fish that fed by 30% concentration of Sargassum sp. flour with the value scale of brightness was 2.67 and the total number of 2,890.67 chromatophore cells. The results suggest that Sargassum sp. powder is better to improve the brightness level of common goldfish scale. Keywords: Carassius auratus auratus, Sargassum sp., Cucurbita moschata, carotenoid, brightness  ABSTRAK Ikan komet Carassius auratus auratus merupakan salah satu spesies ikan hias air tawar yang memiliki nilai jual tinggi. Nilai jual ikan hias dapat ditentukan melalui beberapa parameter, salah satunya yaitu kecerahan sisik ikan. Kecerahan sisik ikan dapat ditingkatkan melalui pemberian pakan yang diperkaya karotenoid. Tujuan penelitian ini adalah menguji tingkat kecerahan warna sisik ikan komet setelah diberi pakan dengan tambahan tepung Sargassum sp. dan

  10. Comparative study of infection with Tetrahymena of different ornamental fish species

    DEFF Research Database (Denmark)

    Sharon, G.; Leibowitz, M. Pimenta; Chettri, Jiwan Kumar

    2014-01-01

    different super orders. The species examined were platy (Xiphophorus), molly (Poecilia sphenops) and angelfish (Pterophyllum scalare) of the Acanthopterygii super order (which also includes guppies) and goldfish (Carassius auratus auratus) and koi carp (Cyprinus carpio) of the Ostariophysi super order....... These two super orders are phylogenetically distant from each other. Infection with Tetrahymena resulted in parasite invasion of internal organs, skin and muscle in all fish species. A relatively strong inflammatory response was observed in infected goldfish and koi, with negligible response in fish species...... of the Acanthopterygii super order. Guppies were the most susceptible to Tetrahymena infection, exhibiting a mortality rate of 87% and 100% in two separate experiments. A high mortality rate was also observed in platy (77%), while that of molly and angelfish was significantly lower (23% and 33%, respectively). Goldfish...

  11. Legacy Bird Species at Risk Monitoring in and Around Camp Navajo and the Naval Observatory Flagstaff Station, AZ

    Science.gov (United States)

    2010-04-16

    19th century due to a convergence of complex stressors such as overgrazing, timber harvest, drought , and fire suppression (Allen et al. 2002, Moore... pinus 16 Olive Warbler Peucedramus taeniatus 5 Plumbeous Vireo Vireo solitarius 60 Pygmy Nuthatch Sitta pygmaea 177 Red Crossbill Loxia...Dove Zenaida macroura 14 Northern Flicker Colaptes auratus 50 Pine Siskin Carduelis pinus 58 Olive-sided Flycatcher Contopus cooperi 1

  12. Colour intensity in angelfish (Pterophyllum scalare) as influenced by dietary microalgae addition

    Czech Academy of Sciences Publication Activity Database

    Kouba, A.; Sales, J.; Sergejevova, Magda; Kozák, P.; Masojídek, Jiří

    2013-01-01

    Roč. 29, č. 1 (2013), s. 193-199 ISSN 0175-8659 R&D Projects: GA ČR GA521/09/0656; GA MŠk(CZ) ED2.1.00/03.0110 Institutional support: RVO:61388971 Keywords : GOLDFISH CARASSIUS-AURATUS * HAEMATOCOCCUS-PLUVIALIS * ASTAXANTHIN Subject RIV: EE - Microbiology, Virology Impact factor: 0.903, year: 2013

  13. American River Watershed Investigation, California. Volume 7. Appendix S. Part 2

    Science.gov (United States)

    1991-12-01

    towhee Pipilo erythrophthalmus C,U,F Brown towhee Pipilo fuscus C,U,F Rufous-crowned sparrow Aimophila ruficeps C,R Chipping sparrow Spizella passerina C,R...pipistrelle Pipistrellus hesperus Widespread in ma habitat Big Brown Bat Eptesicus fuscus Widespread in ma habitat Townsends big-caved bat Plecotus...occidentalis * Carp Qjprjnus ca rv i *Goldfish Carassius auratus Sacramento blackfish Orthodon microlepidotus Hardhead Mylopharodon conocephalus

  14. Radiosensitivity of primary cultured fish cells with different ploidy

    International Nuclear Information System (INIS)

    Mitani, Hiroshi; Egami, Nobuo; Kobayashi, Hiromu.

    1986-01-01

    The radiosensitivity of primary cultured goldfish cells (Carassius auratus) was investigated by colony formation assay. The radiosensitivity of cells from two varieties of goldfish, which show different sensitivity to lethal effect of ionizing radiation in vivo, was almost identical. Primary cultured cells from diploid, triploid and tetraploid fish retained their DNA content as measured by microfluorometry, and the nuclear size increases as ploidy increases. However, radiosensitivity was not related to ploidy. (author)

  15. Environmental Assessment: Addressing Expanded Herbicide Applications and the Relocation of Dry Chemical Testing at Niagra Falls Air Reserve Station, New York

    Science.gov (United States)

    2011-07-01

    description of their uses is provided in the following paragraphs. Roundup ProT" or Kleenup ProT". Both products are post-emergent (i.e., kills existing...cardinalis Northern flicker Colaptes auratus Northern harrier2 Circus cyaneus Northern mockingbird Mimus polyglottos Northern rough-winged swallow...and Kleenup Pro™, when used according to label directions, have no weed killing activity once in contact with the soil. Glyphosate will not move in

  16. Phlebotomus Sandflies of the Paloich Area in the Sudan (Diptera, Psychodidae)

    Science.gov (United States)

    1964-10-01

    Hamster, Cricetus auratus Toad, Bufo regularis (?) Gecko, H. turicus Skink, M. striata Puff adder Snake, CMNH HH-9111’ Snake, CMNH HH-9112’ Hedgehog ...Pharynx unarmed distally or only with few, smail spines ; pigment patch not dark enough to obscure teeth .................. 8 Pharynx heavily...armed distally with numerous, dark spines ; pigment patch very dark, 1964 Quate: Sudanese sandflies 237 often obscuring teeth unless specimen well

  17. Change of nucleolus characteristic of fish embryo cells under the influence of low-level radiation

    International Nuclear Information System (INIS)

    Arkhipchuk, V.V.

    1995-01-01

    The nucleolus activity of fish embryo cells was stimulated by low-level radiation at a dose rate of 2-13 mGy/h. The size of nucleoli generally increased in embryos of Cyprinus carpio, whereas the number of nucleoli was greater in embryos of Carassius auratus gibelio. The higher the functional activity of nucleolus is, the more pronounced are changes in the characteristics. The size of single nucleolus at gastrulation is the most sensitive characteristic. 16 refs.; 1 tab

  18. Susceptibility of Japanese Cyprininae fish species to cyprinid herpesvirus 2 (CyHV-2).

    Science.gov (United States)

    Ito, Takafumi; Maeno, Yukio

    2014-03-14

    Cyprinid herpesvirus 2 (CyHV-2) is known as the causative agent of herpesviral haematopoietic necrosis (HVHN) of goldfish (Carassius auratus). Recently, the virus has also been detected from Prussian carp (C. gibelio) and crucian carp (C. carassius) from European and Asian countries. To analyze the risk of spreading to new host species, the susceptibility of other fish species to the virus is essential. In this study experimental infections of indigenous Cyprininae species in Japan were performed by immersion in and intraperitoneal injection of a CyHV-2 isolate. Although Edonishiki, a variety of goldfish, immersed with the virus showed a cumulative mortality of 90%, no mortality was observed in ginbuna C. auratus langsdorfii, nagabuna C. auratus buergeri, nigorobuna C. auratus grandoculis and common carp Cyprinus carpio. Cumulative mortality was 100, 20 and 10% in intraperitoneally injected Edonishiki, ginbuna and nagabuna, respectively. Furthermore all Edonishiki immersed with the virus died. However, even after stimuli of sudden temperature changes, the immersed ginbuna and nagabuna did not die. Moreover no mortality was observed in co-reared Ranchu, another variety of goldfish, with immersed ginbuna and nagabuna although all three Ranchu co-reared with immersed Edonishiki died. CyHV-2 DNA was detected and the virus was re-isolated from all dead fish. Moreover CyHV-2 DNA was detected from some of the surviving Carassius spp. These results revealed that susceptibility of Japanese indigenous Cyprininae fish species to CyHV-2 is much lower than for goldfish. In addition, ability of replication of CyHV-2 might be different among Carassius fish species. Copyright © 2014 Elsevier B.V. All rights reserved.

  19. Strontium-90 and cesium-137 in freshwater fish (from Jul. 1981 to Dec. 1981)

    International Nuclear Information System (INIS)

    1981-01-01

    90 Sr and 137 Cs in freshwater fish (Carassius auratus, Cyprinus carpio and Hypomesus transpacificus nipponensis) were determined. Fish was rinsed with water and blotted with a filter paper. Only the edible part was used in case of larger sized fish, and the whole part was used in case of smaller ones. The results from July to December 1981 are shown in a table. (Namekawa, K.)

  20. A PTH/PTHrP receptor antagonist blocks the hypercalcemic response to estradiol-17b

    OpenAIRE

    Fuentes, J.; Guerreiro, P. M.; Modesto, Teresa; Rotllant, J.; Canario, Adelino V. M.; Power, Deborah

    2007-01-01

    Estradiol (E2) increases circulating calcium and phosphate levels in fish, thus acting as a hypercalcemic and hyperphosphatemic factor during periods of high calcium requirements, such as during vitellogenesis. Since parathyroid hormone (PTH)-related protein (PTHrP) has been shown to be calciotropic in fish, we hypothesized that the two hormones could be mediating the same process. Sea bream (Sparus auratus) juveniles receiving a single intraperitoneal injection of piscin...