Hanford Site Emergency Alerting System siren testing report

International Nuclear Information System (INIS)

Weidner, L.B.

1997-01-01

The purpose of the test was to determine the effective coverage of the proposed upgrades to the existing Hanford Site Emergency Alerting System (HSEAS). The upgrades are to enhance the existing HSEAS along the Columbia River from the Vernita Bridge to the White Bluffs Boat Launch as well as install a new alerting system in the 400 Area on the Hanford Site. Five siren sites along the Columbia River and two sites in the 400 Area were tested to determine the site locations that will provide the desired coverage

Invasion of Human Oral Epithelial Cells by Prevotella intermedia

Science.gov (United States)

Dorn, Brian R.; Leung, K.-P.; Progulske-Fox, Ann

1998-01-01

Invasion of oral epithelial cells by pathogenic oral bacteria may represent an important virulence factor in the progression of periodontal disease. Here we report that a clinical isolate of Prevotella intermedia, strain 17, was found to invade a human oral epithelial cell line (KB), whereas P. intermedia 27, another clinical isolate, and P. intermedia 25611, the type strain, were not found to invade the cell line. Invasion was quantified by the recovery of viable bacteria following a standard antibiotic protection assay and observed by electron microscopy. Cytochalasin D, cycloheximide, monodansylcadaverine, and low temperature (4°C) inhibited the internalization of P. intermedia 17. Antibodies raised against P. intermedia type C fimbriae and against whole cells inhibited invasion, but the anti-type-C-fimbria antibody inhibited invasion to a greater extent than the anti-whole-cell antibody. This work provides evidence that at least one strain of P. intermedia can invade an oral epithelial cell line and that the type C fimbriae and a cytoskeletal rearrangement are required for this invasion. PMID:9826397

Morphometric and genetic analysis of Arcella intermedia and Arcella intermedia laevis (Amoebozoa, Arcellinida) illuminate phenotypic plasticity in microbial eukaryotes.

Science.gov (United States)

Porfírio-Sousa, Alfredo L; Ribeiro, Giulia M; Lahr, Daniel J G

2017-04-01

Testate amoebae are eukaryotic microorganisms characterized by the presence of an external shell (test). The shell morphology is used as a diagnostic character, but discordance between morphological and molecular data has been demonstrated in groups of arcellinids (Amoebozoa), one of the principal groups of testate amoebae. Morphology of the test is supposed to differentiate genera and species and it is applied in ecological, monitoring and paleontological studies. However, if phenotype does not reflect genotype, conclusions in these types of studies become severely impaired. The objective of this work is to evaluate the morphometrical and morphological variation of the closely related and morphologically similar taxa Arcella intermedia laevis Tsyganov and Mazei, 2006 and Arcella intermedia (Deflandre 1928) Tsyganov and Mazei, 2006 in nature and in cultured individuals and see how these are correlated with molecular data. Our results demonstrate that phenotypic plasticity in Arcella intermedia make morphological distinctions impossible in both taxa. Arcella intermedia and Arcella intermedia laevis are molecularly identical for SSU rDNA and a mitochondrial molecular marker (NAD9/7). We conclude that morphological techniques alone cannot identify phenotypic plasticity from natural populations. More work is clearly needed to better understand the morphological, morphometric and molecular variability in these organisms. Copyright © 2016 Elsevier GmbH. All rights reserved.

Echocardiographic evaluation of thalassemia intermedia patients in Duhok, Iraq.

Science.gov (United States)

Mohammad, Ameen Mosa

2014-12-11

Cardiac complications are among the most serious problems of thalassemia intermedia patients. The current study was initiated to address the latter issue through the study of the echocardiographic findings and correlate it with clinical characteristics of thalassemia intermedia patients in Duhok, Kurdistan region, Iraq. An echocardiographic assessment of 61 beta-thalassemia intermedia cases was performed. It included 30 males and 31 females, with a mean age 19.6 ± 7.5 years. The standard echostudy of two-dimension and M-mode measurements of cardiac chambers were done. The continuous doppler regurgitant jet of tricuspid and pulmonary valves were recorded. Left ventricle diastolic function was assessed by pulsed doppler of mitral valve inflow. To correlate the clinical with echocardiographic findings, patients were divided, according to tricuspid regurgitant velocity, into three groups (intermedia patients. Therapeutic trails targeting these complications are indicated, and echocardiographic assessment is necessary to be offered early for thalassemia intermedia.

Prevotella intermedia induces prostaglandin E2 via multiple signaling pathways.

Science.gov (United States)

Guan, S-M; Fu, S-M; He, J-J; Zhang, M

2011-01-01

Prostaglandin E(2) (PGE(2)) plays important roles in the bone resorption of inflammatory diseases such as rheumatoid arthritis and periodontitis via specific prostaglandin receptors (i.e., EP1-EP4). In this study, the authors examined whether Prevotella intermedia regulates PGE(2) production and EP expression in human periodontal ligament fibroblasts (hPDLs); they also explored the potential signaling pathways involved in PGE(2) production. P. intermedia induced PGE(2) production and cyclooxygenase-2 (COX-2) expression in a dose- and time-dependent manner. Indomethacin and NS-398 completely abrogated the P. intermedia-induced PGE(2) production without modulating COX-2 expression. Specific inhibitors of extracellular signal-regulated kinase, c-Jun N-terminal kinase, p38, phosphatidylinositol 3-kinase, and protein kinase C--but not c-AMP and protein kinase A--significantly attenuated the P. intermedia-induced COX-2 and PGE(2) expression. P. intermedia reduced EP1 expression in a concentration- and time-dependent manner. The results indicate that the COX-2-dependent induction of PGE(2) by P. intermedia in hPDLs is mediated by multiple signaling pathways.

β-Thalassemia intermedia: a comprehensive overview and novel approaches.

Science.gov (United States)

Asadov, Chingiz; Alimirzoeva, Zohra; Mammadova, Tahira; Aliyeva, Gunay; Gafarova, Shahla; Mammadov, Jeyhun

2018-01-29

β-Thalassemia intermedia is a clinical condition of intermediate gravity between β-thalassemia minor, the asymptomatic carrier, and β-thalassemia major, the transfusion-dependent severe anemia. It is characterized by a significant clinical polymorphism, which is attributable to its genetic heterogeneity. Ineffective erythropoiesis, chronic anemia, and iron overload contribute to the clinical complications of thalassemia intermedia through stepwise pathophysiological mechanisms. These complications, including splenomegaly, extramedullary erythropoiesis, iron accumulation, leg ulcers, thrombophilia, and bone abnormalities can be managed via fetal hemoglobin induction, occasional transfusions, chelation, and in some cases, stem cell transplantation. Given its clinical diversity, thalassemia intermedia patients require tailored approaches to therapy. Here we present an overview and novel approaches to the genetic basis, pathophysiological mechanisms, clinical complications, and optimal management of thalassemia intermedia.

Genome sequence of Prevotella intermedia SUNY aB G8-9K-3, a biofilm forming strain with drug-resistance.

Science.gov (United States)

Moon, Ji-Hoi; Kim, Minjung; Lee, Jae-Hyung

Prevotella intermedia has long been known to be as the principal etiologic agent of periodontal diseases and associated with various systemic diseases. Previous studies showed that the intra-species difference exists in capacity of biofilm formation, antibiotic resistance, and serological reaction among P. intermedia strains. Here we report the genome sequence of P. intermedia SUNY aB G8-9K-3 (designated ATCC49046) that displays a relatively high antimicrobial resistant and biofilm-forming capacity. Genome sequencing information provides important clues in understanding the genetic bases of phenotypic differences among P. intermedia strains. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

INTERACTIONS BETWEEN BRANCHIATE MOLE SALAMANDERS (AMBYSTOMA TALPOIDEUM) AND LESSER SIRENS (SIREN INTERMEDIA): ASYMMETRICAL COMPETITION AND INTRAGUILD PREDATION. (R825795)

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

Thalamic Massa Intermedia Duplication in a Dysmorphic 14 month-old Toddler.

Science.gov (United States)

Whitehead, Matthew T

2015-06-01

The massa intermedia is an inconstant parenchymal band connecting the medial thalami. It may be thickened in various disease processes such as Chiari II malformation or absent in other disease states. However, the massa intermedia may also be absent in up to 30% of normal human brains. To the best of my knowledge, detailed imaging findings of massa intermedia duplication have only been described in a single case report. An additional case of thalamic massa intermedia duplication discovered on a routine brain MR performed for dysmorphic facial features is reported herein.

Constraining early and interacting dark energy with gravitational wave standard sirens: the potential of the eLISA mission

International Nuclear Information System (INIS)

Caprini, Chiara; Tamanini, Nicola

2016-01-01

We perform a forecast analysis of the capability of the eLISA space-based interferometer to constrain models of early and interacting dark energy using gravitational wave standard sirens. We employ simulated catalogues of standard sirens given by merging massive black hole binaries visible by eLISA, with an electromagnetic counterpart detectable by future telescopes. We consider three-arms mission designs with arm length of 1, 2 and 5 million km, 5 years of mission duration and the best-level low frequency noise as recently tested by the LISA Pathfinder. Standard sirens with eLISA give access to an intermediate range of redshift 1 ∼< z ∼< 8, and can therefore provide competitive constraints on models where the onset of the deviation from ΛCDM (i.e. the epoch when early dark energy starts to be non-negligible, or when the interaction with dark matter begins) occurs relatively late, at z ∼< 6. If instead early or interacting dark energy is relevant already in the pre-recombination era, current cosmological probes (especially the cosmic microwave background) are more efficient than eLISA in constraining these models, except possibly in the interacting dark energy model if the energy exchange is proportional to the energy density of dark energy.

Antibacterial and antibiofilm effects of iron chelators against Prevotella intermedia.

Science.gov (United States)

Moon, Ji-Hoi; Kim, Cheul; Lee, Hee-Su; Kim, Sung-Woon; Lee, Jin-Yong

2013-09-01

Prevotella intermedia, a major periodontopathogen, has been shown to be resistant to many antibiotics. In the present study, we examined the effect of the FDA-approved iron chelators deferoxamine (DFO) and deferasirox (DFRA) against planktonic and biofilm cells of P. intermedia in order to evaluate the possibility of using these iron chelators as alternative control agents against P. intermedia. DFRA showed strong antimicrobial activity (MIC and MBC values of 0.16 mg ml(-1)) against planktonic P. intermedia. At subMICs, DFRA partially inhibited the bacterial growth and considerably prolonged the bacterial doubling time. DFO was unable to completely inhibit the bacterial growth in the concentration range tested and was not bactericidal. Crystal violet binding assay for the assessment of biofilm formation by P. intermedia showed that DFRA significantly decreased the biofilm-forming activity as well as the biofilm formation, while DFO was less effective. DFRA was chosen for further study. In the ATP-bioluminescent assay, which reflects viable cell counts, subMICs of DFRA significantly decreased the bioactivity of biofilms in a concentration-dependent manner. Under the scanning electron microscope, P. intermedia cells in DFRA-treated biofilm were significantly elongated compared to those in untreated biofilm. Further experiments are necessary to show that iron chelators may be used as a therapeutic agent for periodontal disease.

Beyond concordance cosmology with magnification of gravitational-wave standard sirens.

Science.gov (United States)

Camera, Stefano; Nishizawa, Atsushi

2013-04-12

We show how future gravitational-wave detectors would be able to discriminate between the concordance Λ cold dark matter cosmological model and up-to-date competing alternatives, e.g., dynamical dark energy (DE) models or modified gravity (MG) theories. Our method consists of using the weak-lensing magnification effect that affects a standard-siren signal because of its traveling through the Universe's large scale structure. As a demonstration, we present constraints on DE and MG from proposed gravitational-wave detectors, namely Einstein Telescope and DECI-Hertz Interferometer Gravitational-Wave Observatory and Big-Bang Observer.

Protective effect of an egg yolk-derived immunoglobulin (IgY) against Prevotella intermedia-mediated gingivitis.

Science.gov (United States)

Hou, Y-Y; Zhen, Y-H; Wang, D; Zhu, J; Sun, D-X; Liu, X-T; Wang, H-X; Liu, Y; Long, Y-Y; Shu, X-H

2014-04-01

To investigate the effects of an egg yolk-derived immunoglobulin (IgY) specific to Prevotella intermedia in vitro and in vivo. An IgY specific to P. intermedia was produced by immunizing hens with formaldehyde-inactivated P. intermedia and showed high titres when subjected to an ELISA. The obtained IgY inhibited the growth of P. intermedia in a dose-dependent manner at concentrations from 1 to 20 mg ml(-1) in Center for Disease Control and Prevention liquid medium. Forty rats were challenged with P. intermedia on gingivae and then randomly divided into four groups, which were syringed respectively with phosphate-buffered saline, 1 mg ml(-1) of tinidazole, 20 mg ml(-1) of nonspecific IgY and 20 mg ml(-1) of the IgY specific to P. intermedia at a dosage of 300 μl per day. Gingival index (GI), plaque index (PI), bleeding on probing (BOP), counts of white blood cell (WBC) and histopathological slide of the gums were measured after treatment for 15 days. The gingivitis rats treated with the IgY specific to P. intermedia showed significantly decreased GI, PI, BOP and WBC (P intermedia-mediated gingivitis. A new immunoglobulin specific to P. intermedia was developed from egg yolk. This specific IgY can dose-dependently inhibit the growth of P. intermedia and protect rats from gingivitis induced by P. intermedia. The new IgY has potential for the treatment of P. intermedia-mediated gingivitis. © 2013 The Society for Applied Microbiology.

Issues in Designing a Hypermedia Document System: The Intermedia Case Study.

Science.gov (United States)

Yankelovich, Nicole; And Others

1986-01-01

Intermedia, a hypermedia system developed at Brown University's Institute for Research (Rhode Island) in Information and Scholarship, is first described, and then used as a case study to explore a number of key issues that software designers must consider in the development of hypermedia document systems. A hypermedia document system is defined as…

β-Thalassemia Intermedia: A Clinical Perspective

Science.gov (United States)

Musallam, Khaled M.; Taher, Ali T.; Rachmilewitz, Eliezer A.

2012-01-01

Our understanding of the molecular and pathophysiological mechanisms underlying the disease process in patients with β-thalassemia intermedia has substantially increased over the past decade. Earlier studies observed that patients with β-thalassemia intermedia experience a clinical-complications profile that is different from that in patients with β-thalassemia major. In this article, a variety of clinical morbidities are explored, and their associations with the underlying disease pathophysiology and risk factors are examined. These involve several organs and organ systems including the vasculature, heart, liver, endocrine glands, bone, and the extramedullary hematopoietic system. The effects of some therapeutic interventions on the development of clinical complications are also discussed. PMID:22762026

Chair-side detection of Prevotella Intermedia in mature dental plaque by its fluorescence.

Science.gov (United States)

Nomura, Yoshiaki; Takeuchi, Hiroaki; Okamoto, Masaaki; Sogabe, Kaoru; Okada, Ayako; Hanada, Nobuhiro

2017-06-01

Prevotella intermedia/nigrescens is one of the well-known pathogens causing periodontal diseases, and the red florescence excited by the visible blue light caused by the protoporphyrin IX in the bacterial cells could be useful for the chair-side detection. The aim of this study was to evaluated levels of periodontal pathogen, especially P. intermedia in clinical samples of red fluorescent dental plaque. Thirty two supra gingival plaque samples from six individuals were measured its fluorescence at 640nm wavelength excited by 409nm. Periodontopathic bacteria were counted by the Invader PLUS PCR assay. Co-relations the fluorescence intensity and bacterial counts were analyzed by Person's correlation coefficient and simple and multiple regression analysis. Positive and negative predictive values of the fluorescence intensities for with or without P. intermedia in supragingival plaque was calculated. When relative fluorescence unit (RFU) were logarithmic transformed, statistically significant linear relations between RFU and bacterial counts were obtained for P. intermedia, Porphyromonas gingivalis and Tannerella forsythia. By the multiple regression analysis, only P. intermedia had statistically significant co-relation with fluorescence intensities. All of the fluorescent dental plaque contained P. intermedia m. In contrast, 28% of non-fluorescent plaques contained P. intermedia. To check the fluorescence dental plaque in the oral cavity could be the simple chair-side screening of the mature dental plaque before examining the periodontal pathogens especially P. intermedia by the PCR method. Copyright © 2017 Elsevier B.V. All rights reserved.

Science with the space-based interferometer eLISA. III: probing the expansion of the universe using gravitational wave standard sirens

Energy Technology Data Exchange (ETDEWEB)

Tamanini, Nicola; Caprini, Chiara [Institut de Physique Théorique, CEA-Saclay, CNRS UMR 3681, Université Paris-Saclay, F-91191 Gif-sur-Yvette (France); Barausse, Enrico [Sorbonne Universités, UPMC Université Paris 6, UMR 7095, Institut d' Astrophysique de Paris, 98 bis Bd Arago, 75014 Paris (France); Sesana, Alberto [School of Physics and Astronomy, The University of Birmingham, Edgbaston, Birmingham B15 2TT (United Kingdom); Klein, Antoine [Department of Physics and Astronomy, The University of Mississippi, University, MS 38677 (United States); Petiteau, Antoine, E-mail: nicola.tamanini@cea.fr, E-mail: chiara.caprini@cea.fr, E-mail: barausse@iap.fr, E-mail: asesana@star.sr.bham.ac.uk, E-mail: aklein@physics.montana.edu, E-mail: antoine.petiteau@apc.univ-paris7.fr [APC, Université Paris Diderot, Observatoire de Paris, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205 Paris Cedex 13 (France)

2016-04-01

We investigate the capability of various configurations of the space interferometer eLISA to probe the late-time background expansion of the universe using gravitational wave standard sirens. We simulate catalogues of standard sirens composed by massive black hole binaries whose gravitational radiation is detectable by eLISA, and which are likely to produce an electromagnetic counterpart observable by future surveys. The main issue for the identification of a counterpart resides in the capability of obtaining an accurate enough sky localisation with eLISA. This seriously challenges the capability of four-link (2 arm) configurations to successfully constrain the cosmological parameters. Conversely, six-link (3 arm) configurations have the potential to provide a test of the expansion of the universe up to z ∼ 8 which is complementary to other cosmological probes based on electromagnetic observations only. In particular, in the most favourable scenarios, they can provide a significant constraint on H{sub 0} at the level of 0.5%. Furthermore, (Ω{sub M}, Ω{sub Λ}) can be constrained to a level competitive with present SNIa results. On the other hand, the lack of massive black hole binary standard sirens at low redshift allows to constrain dark energy only at the level of few percent.

Tropical House Gecko (Hemidactylus mabouia predation on brown spiders (Loxosceles intermedia

Directory of Open Access Journals (Sweden)

E.N. Ramires

2004-01-01

Full Text Available Brown spiders (Loxosceles spp. are venomous arachnids, successfully adapted to urban habitats in Brazil. Loxoscelism became a serious public health problem in Paraná State, especially at the capital Curitiba, where the most abundant species is Loxosceles intermedia. Hemidactylus mabouia (Gekkonidae lizards are synanthropic predators of arthropods. In this paper, we describe the predatory behavior of the Tropical House Gecko H. mabouia on L. intermedia under laboratory conditions. Twelve geckos were observed, and all of them fed on brown spiders (n=123 observations. The attack consisted of a fast run followed by one bite on the spider’s abdomen or legs. The geckos did not attack L. intermedia anterior body parts, probably due to the fangs present in this region. Two Hemidactylus individuals were killed by L. intermedia bites: during a predatory encounter, and by an induced bite on a restrained lizard. The observations summarized in this paper show that H. mabouia could be used in the biological control of Loxosceles populations in human dwellings. However, additional field studies are necessary to quantify the impact of H. mabouia predation on urban populations of L. intermedia and other species of the same genus.

Ramalina intermedia in Nederland

NARCIS (Netherlands)

Segal, S.

1968-01-01

The lichen Ramalina intermedia Del. apud Lamy was found near Apeldoorn on a decayed wall of an old castle in the possession of the Royal family. The wall, rising up from the castle moat, dates from the 16th century, but remained covered with soil for several centuries after the moat had been filled

Prevotella intermedia and Porphyromonas gingivalis in dental caries with periapical granuloma

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Risya Cilmiaty

2013-12-01

Full Text Available Background: Dental caries with necrotic pulp is a multifactorial disease that attacks enamel involving tooth pulp. The anaerobic bacteria infection in the pulp chamber could induce the formation of periapical granuloma. However, the presence of the most frequently anaerobic bacteria identified in apical periodontitis, Porphyromonas gingivalis and Prevotella intermedia, in periapical granuloma have not been confirmed. Purpose: The aims of study were to determine the presence of Porphyromonas gingivalis and Prevotella intermedia in dental caries with necrotic pulp and to determine its relation to periapical granuloma. Methods: Thirty-six patients of dental caries with necrotic pulp in Dr. Moewardi General Hospital in Surakarta, Indonesia were involved and classified into two groups, the group of patients with periapical granuloma and the group of patients without periapical granuloma. The caries tooth was extracted, and the chronic periapical tissue was swabbed and cultured on blood agar medium in anaerobic condition. The bacterial DNA was extracted from the positive cultures and subjected for Polymerase Chain Reaction (PCR. Results: Periapical granuloma was more likely found in women (OR 5.5, 95% CI=1.277-23.693; RR 2.5, 95% CI= 1.025-6.100. Black colonies bacteria were associated with periapical granuloma (OR 2.2, 95% CI=0.517-9.594; RR 1.5, 95% CI=0.655-3.623. Porphyromonas gingivalis and Prevotella intermedia were detected in group with or without periapical granuloma, however, only Prevotella intermedia was associated with periapical granuloma (OR 1.6, 95% CI=0.418-5.903; RR 1.3, 95% CI=0.653-2.393. Conclusion: The presence of Porphyromonas gingivalis and Prevotella intermedia in periapical granuloma were confirmed, however, only Prevotella intermedia were associated with periapical granuloma.Latar belakang: Karies gigi dengan pulpa nekrosis adalah penyakit multifaktorial yang menyerang enamel hingga ruang pulpa gigi. Infeksi bakteri anaerob

BITING IN THE SALAMANDER SIREN INTERMEDIA: COURTSHIP COMPONENT OR AGONISTIC BEHAVIOR? (R825795)

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

Development and evaluation of new primers for PCR-based identification of Prevotella intermedia.

Science.gov (United States)

Zhou, Yanbin; Liu, Dali; Wang, Yiwei; Zhu, Cailian; Liang, Jingping; Shu, Rong

2014-08-01

The aim of this study was to develop new Prevotella intermedia-specific PCR primers based on the 16S rRNA. The new primer set, Pi-192 and Pi-468, increased the accuracy of PCR-based P. intermedia identification and could be useful in the detection of P. intermedia as well as epidemiological studies on periodontal disease. Copyright © 2014 Elsevier Ltd. All rights reserved.

Acoustic signals in the sand fly Lutzomyia (Nyssomyia) intermedia (Diptera: Psychodidae).

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Vigoder, Felipe M; Souza, Nataly A; Peixoto, Alexandre A

2011-05-13

Acoustic signals are part of the courtship of many insects and they often act as species-specific signals that are important in the reproductive isolation of closely related species. Here we report the courtship songs of the sand fly Lutzomyia (Nyssomyia) intermedia, one of the main vectors of cutaneous leishmaniasis in Brazil. Recordings were performed using insects from three localities from Eastern Brazil: Posse and Jacarepaguá in Rio de Janeiro State and Corte de Pedra in Bahia State. The three areas have remnants of the Brazilian Atlantic forest, they are endemic for cutaneous leishmaniasis and L. intermedia is the predominant sand fly species. We observed that during courtship L. intermedia males from all populations produced pulse songs consisting of short trains. No significant differences in song parameters were observed between the males of the three localities. L. intermedia males produce acoustic signals as reported for some other sand flies such as the sibling species of the Lutzomyia longipalpis complex. The lack of differences between the males from the three localities is consistent with previous molecular studies of the period gene carried out in the same populations, reinforcing the idea that L. intermedia is not a species complex in the studied areas and that the three populations are likely to have similar vectorial capacities.

Sobre a dispersão de Lutzomyia Intermedia (Diptera, Psychodidae The dispersion of Lutzomyia intermedia (Diptera, Psychodidae

Directory of Open Access Journals (Sweden)

Mario B. Aragão

1987-12-01

Full Text Available Utilizando um mapa de domínios morfoclimáticos e um de vegetação, foram estudadas as áreas de dispersão da espécie e obtidas as seguintes conclusões: 1 - Lutzomyia intermedia é um flebótomo que ocorre nas florestas que apresentam o seu máximo de desenvolvimento nas encostas úmidas, mas, que ocupam também áreas de relevo pouco movimentadas ou planas. 2 - No Brasil, essas encostas pertencem às serras do Mar e da Mantiqueira e, no noroeste da Argentina, situam-se na Cordilheira dos Andes. 3 - E provável que a espécie exista nas serras florestadas do interior do nordeste brasileiro.Using vegetation and morphoclimactic domain maps the areas of dispersion of Lutzomyia intermedia were studied and the following conclusions were obtained: 1 - L. intermedia is a sandfly of the forests which have their maximum growth at the humid slopes although these forests can also be found in plains and areas with low hills. 2 - In Brazil these slopes are found at the Serras do Mar and Mantiqueira and in Argentina ath the Andes Mountains in the northwest of the country. 3 - This species probably occurs also in the forested mountainous areas of the northeastern region of Brazil.

The identification of genes specific to Prevotella intermedia and Prevotella nigrescens using genomic subtractive hybridization.

Science.gov (United States)

Masakiyo, Yoshiaki; Yoshida, Akihiro; Shintani, Yasuyuki; Takahashi, Yusuke; Ansai, Toshihiro; Takehara, Tadamichi

2010-06-01

Prevotella intermedia and Prevotella nigrescens, which are often isolated from periodontal sites, were once considered two different genotypes of P. intermedia. Although the genomic sequence of P. intermedia was determined recently, little is known about the genetic differences between P. intermedia and P. nigrescens. The subtractive hybridization technique is a powerful method for generating a set of DNA fragments differing between two closely related bacterial strains or species. We used subtractive hybridization to identify the DNA regions specific to P. intermedia ATCC 25611 and P. nigrescens ATCC 25261. Using this method, four P. intermedia ATCC 25611-specific and three P. nigrescens ATCC 25261-specific regions were determined. From the species-specific regions, insertion sequence (IS) elements were isolated for P. intermedia. IS elements play an important role in the pathogenicity of bacteria. For the P. intermedia-specific regions, the genes adenine-specific DNA-methyltransferase and 8-amino-7-oxononanoate synthase were isolated. The P. nigrescens-specific region contained a Flavobacterium psychrophilum SprA homologue, a cell-surface protein involved in gliding motility, Prevotella melaninogenica ATCC 25845 glutathione peroxide, and Porphyromonas gingivalis ATCC 33277 leucyl-tRNA synthetase. The results demonstrate that the subtractive hybridization technique was useful for distinguishing between the two closely related species. Furthermore, this technique will contribute to our understanding of the virulence of these species. 2009 Elsevier Ltd. All rights reserved.

The pars intermedia: an anatomic basis for a coordinated vascular response to female genital arousal.

Science.gov (United States)

Shih, Cheryl; Cold, Christopher J; Yang, Claire C

2013-06-01

The pars intermedia is an area of the vulva that has been inconsistently described in the literature. We conducted anatomic studies to better describe the tissues and vascular structures of the pars intermedia and proposed a functional rationale of the pars intermedia in the female sexual response. Nine cadaveric vulvectomy specimens were used. Each was serially sectioned and stained with hematoxylin and eosin and Masson's trichrome. Histologic ultrastructural description of the pars intermedia. The pars intermedia contains veins traveling longitudinally in the angle of the clitoris, supported by collagen-rich stromal tissues. These veins drain the different vascular compartments of the vulva, including the clitoris, the bulbs, and labia minora; also, the interconnecting veins link the different vascular compartments. The pars intermedia is not composed of erectile tissue, distinguishing it from the erectile tissues of the corpora cavernosa of the clitoris as well as the corpus spongiosum of the clitoral (vestibular) bulbs. The venous communications of the pars intermedia, linking the erectile tissues with the other vascular compartments of the vulva, appear to provide the anatomic basis for a coordinated vascular response during female sexual arousal. © 2012 International Society for Sexual Medicine.

Fluxacademy: From Intermedia to Interactive Education.

Science.gov (United States)

Saper, Craig

1992-01-01

Advocates a Fluxus-based experimental pedagogy which is suited for scholarship confronted with film and electronic media. Notes that the theory explored in Fluxacademy focuses specifically on the use of intermedia for interactive education. (RS)

[Genetic relationship analysis of Ephedra intermedia from different habitat in Gansu by ISSR analysis].

Science.gov (United States)

Zhu, Tian-Tian; Jin, Ling; Du, Tao; Cui, Zhi-Jia; Zhang, Xian-Fei; Wu, Di

2013-09-01

To investigate the genetic relationship of Ephedra intermedia from different habitats in Gansu. The genetic diversity and genetic relationship of E. intermedia from different habitats in Gansu were studied by ISSR molecular marker technique. Twelve ISSR primers were selected from 70 ISSR primers and used for ISSR amplification. Total 112 loci were amplified, in which 81 were polymorphic loci, the average percentage of polymorphie bands (PPB) was 72.32%. Clustering results indicated that the wild species and cultivating species were clustered into different group. The wild species, which had closer distance, were clustered into a group. E. intermedia of different habitats in Gansu have rich genetic diversities among species, it is the reason that E. intermedia has strong adaptability and wide distribution. Further, the genetic distance of E. intermedia is associated with geographical distance, the further distance can hinder the gene flow.

Connection of Gnomonia intermedia to Discula betulina and its relationship to other taxa in Gnomoniaceae.

Science.gov (United States)

Green, Sarah; Castlebury, Lisa A

2007-01-01

Discula betulina is a foliar pathogen on birch (Betula) and Gnomonia intermedia is found on overwintered birch leaves. Perithecia of G. intermedia developed in vitro on colonies of D. betulina isolated from birch tissues in late summer, and single ascospores of G. intermedia consistently developed into colonies similar to D. betulina, producing typical D. betulina conidia. Isolates of D. betulina could be grouped into two mating types, which produced fertile perithecia of G. intermedia when mated with each other. Mycelia from single-ascospore and single-conidial isolates were inoculated onto shoots of downy birch, causing lesions and die-back from which D. betulina was consistently isolated. ITS region ribosomal DNA sequence analysis confirmed the results of the morphological and mating studies, and found that the closest known relatives of G. intermedia/D. betulina are Gnomoniella nana and Sirococcus clavigignenti-juglandacearum. The conclusion from these studies is that D. betulina is the anamorph of G. intermedia.

Acoustic signals in the sand fly Lutzomyia (Nyssomyia intermedia (Diptera: Psychodidae

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Peixoto Alexandre A

2011-05-01

Full Text Available Abstract Background Acoustic signals are part of the courtship of many insects and they often act as species-specific signals that are important in the reproductive isolation of closely related species. Here we report the courtship songs of the sand fly Lutzomyia (Nyssomyia intermedia, one of the main vectors of cutaneous leishmaniasis in Brazil. Findings Recordings were performed using insects from three localities from Eastern Brazil: Posse and Jacarepaguá in Rio de Janeiro State and Corte de Pedra in Bahia State. The three areas have remnants of the Brazilian Atlantic forest, they are endemic for cutaneous leishmaniasis and L. intermedia is the predominant sand fly species. We observed that during courtship L. intermedia males from all populations produced pulse songs consisting of short trains. No significant differences in song parameters were observed between the males of the three localities. Conclusions L. intermedia males produce acoustic signals as reported for some other sand flies such as the sibling species of the Lutzomyia longipalpis complex. The lack of differences between the males from the three localities is consistent with previous molecular studies of the period gene carried out in the same populations, reinforcing the idea that L. intermedia is not a species complex in the studied areas and that the three populations are likely to have similar vectorial capacities.

Acoustic signals in the sand fly Lutzomyia (Nyssomyia) intermedia (Diptera: Psychodidae)

Science.gov (United States)

2011-01-01

Background Acoustic signals are part of the courtship of many insects and they often act as species-specific signals that are important in the reproductive isolation of closely related species. Here we report the courtship songs of the sand fly Lutzomyia (Nyssomyia) intermedia, one of the main vectors of cutaneous leishmaniasis in Brazil. Findings Recordings were performed using insects from three localities from Eastern Brazil: Posse and Jacarepaguá in Rio de Janeiro State and Corte de Pedra in Bahia State. The three areas have remnants of the Brazilian Atlantic forest, they are endemic for cutaneous leishmaniasis and L. intermedia is the predominant sand fly species. We observed that during courtship L. intermedia males from all populations produced pulse songs consisting of short trains. No significant differences in song parameters were observed between the males of the three localities. Conclusions L. intermedia males produce acoustic signals as reported for some other sand flies such as the sibling species of the Lutzomyia longipalpis complex. The lack of differences between the males from the three localities is consistent with previous molecular studies of the period gene carried out in the same populations, reinforcing the idea that L. intermedia is not a species complex in the studied areas and that the three populations are likely to have similar vectorial capacities. PMID:21569534

Draft genome sequence of a Kluyvera intermedia isolate from a patient with a pancreatic abscess

DEFF Research Database (Denmark)

Thele, Roland; Gumpert, Heidi; Christensen, Louise B.

2017-01-01

The genus Kluyvera comprises potential pathogens that can cause many infections. This study reports a Kluyvera intermedia strain (FOSA7093) from a pancreatic cyst specimen from a long-term hospitalised patient. Whole-genome sequencing (WGS) of the K. intermedia isolate was performed and the strain...... an efficient tool for monitoring Kluyvera spp. and its role as a reservoir of multidrug resistance. Therefore, this susceptible K. intermedia genome has many characteristics that allow comparison of resistant K. intermedia that might be discovered in the future....

Control of postharvest Botrytis fruit rot of strawberry by volatile organic compounds of Candida intermedia.

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Huang, R; Li, G Q; Zhang, J; Yang, L; Che, H J; Jiang, D H; Huang, H C

2011-07-01

A study was conducted to identify volatile organic compounds or volatiles produced by Candida intermedia strain C410 using gas chromatography-mass spectrometry, and to determine efficacy of the volatiles of C. intermedia in suppression of conidial germination and mycelial growth of Botrytis cinerea and control of Botrytis fruit rot of strawberry. Results showed that, among 49 volatiles (esters, alcohols, alkenes, alkanes, alkynes, organic acids, ketones, and aldehydes) identified from C. intermedia cultures on yeast extract peptone dextrose agar, two compounds, 1,3,5,7-cyclooctatetraene and 3-methyl-1-butanol, were the most abundant. Synthetic chemicals of 1,3,5,7-cyclooctatetraene; 3-methyl-1-butanol; 2-nonanone; pentanoic acid, 4-methyl-, ethyl ester; 3-methyl-1-butanol, acetate; acetic acid, pentyl ester; and hexanoic acid, ethyl ester were highly inhibitory to conidial germination and mycelial growth of B. cinerea. Inhibition of conidial germination and mycelial growth of B. cinerea by volatiles of C. intermedia was also observed. Meanwhile, results showed that incidence and severity of Botrytis fruit rot of strawberry was significantly (P intermedia cultures or C. intermedia-infested strawberry fruit. These results suggest that the volatiles of C. intermedia C410 are promising biofumigants for control of Botrytis fruit rot of strawberry.

In Vitro Effects of Polyphosphate against Prevotella intermedia in Planktonic Phase and Biofilm.

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Jang, Eun-Young; Kim, Minjung; Noh, Mi Hee; Moon, Ji-Hoi; Lee, Jin-Yong

2016-02-01

Polyphosphate (polyP) has gained a wide interest in the food industry due to its potential as a decontaminating agent. In this study, we examined the effect of sodium tripolyphosphate (polyP3; Na5P3O10) against planktonic and biofilm cells of Prevotella intermedia, a major oral pathogen. The MIC of polyP3 against P. intermedia ATCC 49046 determined by agar dilution method was 0.075%, while 0.05% polyP3 was bactericidal against P. intermedia in time-kill analysis performed using liquid medium. A crystal violet binding assay for the assessment of biofilm formation by P. intermedia showed that sub-MICs of polyP3 significantly decreased biofilm formation. Under the scanning electron microscope, decreased numbers of P. intermedia cells forming the biofilms were observed when the bacterial cells were incubated with 0.025% or higher concentrations of polyP3. Assessment of biofilm viability with LIVE/DEAD staining and viable cell count methods showed that 0.05% or higher concentrations of polyP3 significantly decreased the viability of the preformed biofilms in a concentration-dependent manner. The zone sizes of alpha-hemolysis formed on horse blood agar produced by P. intermedia were decreased in the presence of polyP3. The expression of the genes encoding hemolysins and the genes of the hemin uptake (hmu) locus was downregulated by polyP3. Collectively, our results show that polyP is an effective antimicrobial agent against P. intermedia in biofilms as well as planktonic phase, interfering with the process of hemin acquisition by the bacterium. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Stellar binary black holes in the LISA band: a new class of standard sirens

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Del Pozzo, Walter; Sesana, Alberto; Klein, Antoine

2018-04-01

The recent Advanced LIGO detections of coalescing black hole binaries (BHBs) imply a large population of such systems emitting at milli-Hz frequencies, accessible to the Laser Interferometer Space Antenna (LISA). We show that these systems provide a new class of cosmological standard sirens. Direct LISA luminosity distance - Dl - measurements, combined with the inhomogeneous redshift - z - distribution of possible host galaxies provide an effective way to populate the Dl-z diagram at z arm-length, respectively.

Thalidomide has a significant effect in patients with thalassemia intermedia.

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Li, YunShuan; Ren, Quan; Zhou, Yali; Li, Pingping; Lin, Wanhua; Yin, Xiaolin

2018-01-01

To investigate the effect of thalidomide in patients with thalassemia intermedia. We observed the effect of thalidomide in seven patients with thalassemia intermedia requiring blood transfusion. Four of the patients were transfusion-independent, and three patients were transfusion-dependent. For the four transfusion-independent patients, hemoglobin concentration increased significantly (≥2 g/dl) in three and moderately (1-2 g/dl) in one. After 3 months of treatment, hemoglobin concentration increased 3.2 ± 1.2 g/dl compared to pretreatment. Among the three transfusion-dependent patients, transfusion was terminated after one month of treatment in one patient and decreased >50% in the other two patients, accompanied by an increase in the average hemoglobin concentration. Thalidomide had a significant effect in patients with thalassemia intermedia. Further studies of a larger scale and more rigorous design are warranted.

Leptin potentiates Prevotella intermedia lipopolysaccharide-induced production of TNF-alpha in monocyte-derived macrophages.

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Kim, Sung-Jo

2010-06-01

In addition to regulating body weight, leptin is also recognized for its role in the regulation of immune function and inflammation. The purpose of this study was to investigate the effect of leptin on Prevotella (P.) intermedia lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-alpha production in differentiated THP-1 cells, a human monocytic cell line. LPS from P. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. THP-1 cells were incubated in the medium supplemented with phorbol myristate acetate to induce differentiation into macrophage-like cells. The amount of TNF-alpha and interleukin-8 secreted into the culture medium was determined by enzyme-linked immunosorbent assay (ELISA). TNF-alpha and Ob-R mRNA expression levels were determined by semi-quantitative reverse transcription-polymerase chain reaction analysis. Leptin enhanced P. intermedia LPS-induced TNF-alpha production in a dose-dependent manner. Leptin modulated P. intermedia LPS-induced TNF-alpha expression predominantly at the transcriptional level. Effect of leptin on P. intermedia LPS-induced TNF-alpha production was not mediated by the leptin receptor. The ability of leptin to enhance P. intermedia LPS-induced TNF-alpha production may be important in the establishment of chronic lesion accompanied by osseous tissue destruction observed in inflammatory periodontal disease.

Nucleases from Prevotella intermedia can degrade neutrophil extracellular traps.

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Doke, M; Fukamachi, H; Morisaki, H; Arimoto, T; Kataoka, H; Kuwata, H

2017-08-01

Periodontitis is an inflammatory disease caused by periodontal bacteria in subgingival plaque. These bacteria are able to colonize the periodontal region by evading the host immune response. Neutrophils, the host's first line of defense against infection, use various strategies to kill invading pathogens, including neutrophil extracellular traps (NETs). These are extracellular net-like fibers comprising DNA and antimicrobial components such as histones, LL-37, defensins, myeloperoxidase, and neutrophil elastase from neutrophils that disarm and kill bacteria extracellularly. Bacterial nuclease degrades the NETs to escape NET killing. It has now been shown that extracellular nucleases enable bacteria to evade this host antimicrobial mechanism, leading to increased pathogenicity. Here, we compared the DNA degradation activity of major Gram-negative periodontopathogenic bacteria, Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. We found that Pr. intermedia showed the highest DNA degradation activity. A genome search of Pr. intermedia revealed the presence of two genes, nucA and nucD, putatively encoding secreted nucleases, although their enzymatic and biological activities are unknown. We cloned nucA- and nucD-encoding nucleases from Pr. intermedia ATCC 25611 and characterized their gene products. Recombinant NucA and NucD digested DNA and RNA, which required both Mg 2+ and Ca 2+ for optimal activity. In addition, NucA and NucD were able to degrade the DNA matrix comprising NETs. © 2016 The Authors Molecular Oral Microbiology Published by John Wiley & Sons Ltd.

Comparison of the virulence of exopolysaccharide-producing Prevotella intermedia to exopolysaccharide non-producing periodontopathic organisms.

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Yamanaka, Takeshi; Yamane, Kazuyoshi; Furukawa, Tomoyo; Matsumoto-Mashimo, Chiho; Sugimori, Chieko; Nambu, Takayuki; Obata, Noboru; Walker, Clay B; Leung, Kai-Poon; Fukushima, Hisanori

2011-08-25

Evidence in the literature suggests that exopolysaccharides (EPS) produced by bacterial cells are essential for the expression of virulence in these organisms. Secreted EPSs form the framework in which microbial biofilms are built. This study evaluates the role of EPS in Prevotella intermedia for the expression of virulence. This evaluation was accomplished by comparing EPS-producing P. intermedia strains 17 and OD1-16 with non-producing P. intermedia ATCC 25611 and Porphyromonas gingivalis strains ATCC 33277, 381 and W83 for their ability to induce abscess formation in mice and evade phagocytosis. EPS-producing P. intermedia strains 17 and OD1-16 induced highly noticeable abscess lesions in mice at 107 colony-forming units (CFU). In comparison, P. intermedia ATCC 25611 and P. gingivalis ATCC 33277, 381 and W83, which all lacked the ability to produce viscous materials, required 100-fold more bacteria (109 CFU) in order to induce detectable abscess lesions in mice. Regarding antiphagocytic activity, P. intermedia strains 17 and OD1-16 were rarely internalized by human polymorphonuclear leukocytes, but other strains were readily engulfed and detected in the phagosomes of these phagocytes. These results demonstrate that the production of EPS by P. intermedia strains 17 and OD1-16 could contribute to the pathogenicity of this organism by conferring their ability to evade the host's innate defence response.

Characterization of Atypical Isolates of Yersinia intermedia and Definition of Two New Biotypes▿ †

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Martin, Liliane; Leclercq, Alexandre; Savin, Cyril; Carniel, Elisabeth

2009-01-01

The species Yersinia intermedia is a member of the genus Yersinia which belongs to the Enterobacteriaceae family. This species is divided into eight biotypes, according to Brenner's biotyping scheme. This scheme relies on five tests (utilization of Simmons citrate and acid production from d-melibiose, d-raffinose, α-methyl-d-glucoside [αMG], and l-rhamnose). The collection of the French Yersinia Reference Laboratory (Institut Pasteur, Paris, France) contained 44 strains that were originally identified as Y. intermedia but whose characteristics did not fit into the biotyping scheme. These 44 strains were separated into two biochemical groups: variant 1 (positive for acid production from l-rhamnose and αMG and positive for Simmons citrate utlization) and variant 2 (positive for acid production from l-rhamnose and αMG). These atypical strains could correspond to new biotypes of Y. intermedia, to Y. frederiksenii strains having the atypical property of fermenting αMG, or to new Yersinia species. These strains did not exhibit growth or phenotypic properties different from those of Y. intermedia and Y. frederiksenii and did not harbor any of the virulence traits usually found in pathogenic species. DNA-DNA hybridizations performed between one strain each of variants 1 and 2 and the Y. intermedia and Y. frederiksenii type strains demonstrated that these variants do belong to the Y. intermedia species. We thus propose that Brenner's biotyping scheme be updated by adding two new biotypes: 9 (for variant 1) and 10 (for variant 2) to the species Y. intermedia. PMID:19494062

Splenectomy for people with thalassaemia major or intermedia.

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Easow Mathew, Manu; Sharma, Akshay; Aravindakshan, Rajeev

2016-06-14

surgery. Given the lack of detail regarding the study methods beyond randomisation, the overall risk of bias for this study was unclear. The study was carried out over a period of 3.5 years, with each participant followed up only until discharge (less than one week after the intervention); it did not assess the majority of the outcomes outlined in this review (including two of the three primary outcomes, frequency of transfusion and quality of life). A total of three serious post-operative adverse events (the review's third primary outcome) were reported in the laparoscopic splenectomy group (one case of atelectasis and two cases of bleeding), compared to two events of atelectasis in the open surgery group; however, there were no significant differences between the groups for either atelectasis, risk ratio 0.50 (95% confidence interval 0.05 to 4.90) or for bleeding, risk ratio 5.00 (95% confidence interval 0.26 to 95.61). In addition, the study also reported three serious cases of intra-operative bleeding in the laparoscopic group which mandated conversion to open surgery, although the difference between groups was not statistically significant, risk ratio 7.00 (95% confidence interval 0.39 to 124.14). These effect estimates are based on very small numbers and hence are unreliable and imprecise. From this small study, there appeared to be an advantage for the laparoscopic approach, in terms of post-operative hospital stay, although the group difference was not large (median difference of 1.5 days, P = 0.03). The review was unable to find good quality evidence, in the form of randomised controlled studies, regarding the efficacy of splenectomy for treating thalassaemia major or intermedia. The single included study provided little information about the efficacy of splenectomy, and compared open surgery and laparoscopic methods. Further studies need to evaluate the long-term effectiveness of splenectomy and the comparative advantages of surgical methods. Due to a lack of

Public Safety Transmitter Towers, Civil Service emergency siren locations within Sedgwick County. Cover is maintained interactively by GIS staff. Primary attributes include siren ID, class, range, model, description, ownership, control, address, city, and GPS verification status. Publi, Published in 2008, 1:1200 (1in=100ft) scale, Sedgwick County Government.

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NSGIC Local Govt | GIS Inventory — Public Safety Transmitter Towers dataset current as of 2008. Civil Service emergency siren locations within Sedgwick County. Cover is maintained interactively by GIS...

Development of Lutzomyia intermedia and Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae larvae in different diets

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WERMELINGER E. D.

2001-01-01

Full Text Available The objective of this research was to evaluate, in laboratory, the development of Lutzomyia intermedia and Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominae larvae, vectors of leishmaniasis in Brazil, in the following diets: industrialized food for rabbits, dogs, hamsters and aquarium fishes, besides liver powder, cooked lettuce, wheat germ, beer yeast, oat, wheat bran and a diet denominated aged food. Except wheat bran for L. intermedia, all diets provided adequate development for both species, which showed that any of them can be used in laboratory insectaries for these insects. L. intermedia showed better development with most nutritious diets and both species presented better development with aged food. Fungi as an additional nutrient source for L. intermedia and L. longipalpis is suggested.

Development of Prevotella intermedia-specific PCR primers based on the nucleotide sequences of a DNA probe Pig27.

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Kim, Min Jung; Hwang, Kyung Hwan; Lee, Young-Seok; Park, Jae-Yoon; Kook, Joong-Ki

2011-03-01

The aim of this study was to develop Prevotella intermedia-specific PCR primers based on the P. intermedia-specific DNA probe. The P. intermedia-specific DNA probe was screened by inverted dot blot hybridization and confirmed by Southern blot hybridization. The nucleotide sequences of the species-specific DNA probes were determined using a chain termination method. Southern blot analysis showed that the DNA probe, Pig27, detected only the genomic DNA of P. intermedia strains. PCR showed that the PCR primers, Pin-F1/Pin-R1, had species-specificity for P. intermedia. The detection limits of the PCR primer sets were 0.4pg of the purified genomic DNA of P. intermedia ATCC 49046. These results suggest that the PCR primers, Pin-F1/Pin-R1, could be useful in the detection of P. intermedia as well as in the development of a PCR kit in epidemiological studies related to periodontal diseases. Crown Copyright © 2010. Published by Elsevier B.V. All rights reserved.

Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective salivary protein against Leishmania braziliensis infection.

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de Moura, Tatiana R; Oliveira, Fabiano; Carneiro, Marcia W; Miranda, José Carlos; Clarêncio, Jorge; Barral-Netto, Manoel; Brodskyn, Cláudia; Barral, Aldina; Ribeiro, José M C; Valenzuela, Jesus G; de Oliveira, Camila I

2013-01-01

Leishmania parasites are transmitted in the presence of sand fly saliva. Together with the parasite, the sand fly injects salivary components that change the environment at the feeding site. Mice immunized with Phlebotomus papatasi salivary gland (SG) homogenate are protected against Leishmania major infection, while immunity to Lutzomyia intermedia SG homogenate exacerbated experimental Leishmania braziliensis infection. In humans, antibodies to Lu. intermedia saliva are associated with risk of acquiring L. braziliensis infection. Despite these important findings, there is no information regarding the repertoire of Lu. intermedia salivary proteins. A cDNA library from the Salivary Glands (SGs) of wild-caught Lu. intermedia was constructed, sequenced, and complemented by a proteomic approach based on 1D SDS PAGE and mass/mass spectrometry to validate the transcripts present in this cDNA library. We identified the most abundant transcripts and proteins reported in other sand fly species as well as novel proteins such as neurotoxin-like proteins, peptides with ML domain, and three small peptides found so far only in this sand fly species. DNA plasmids coding for ten selected transcripts were constructed and used to immunize BALB/c mice to study their immunogenicity. Plasmid Linb-11--coding for a 4.5-kDa protein--induced a cellular immune response and conferred protection against L. braziliensis infection. This protection correlated with a decreased parasite load and an increased frequency of IFN-γ-producing cells. We identified the most abundant and novel proteins present in the SGs of Lu. intermedia, a vector of cutaneous leishmaniasis in the Americas. We also show for the first time that immunity to a single salivary protein from Lu. intermedia can protect against cutaneous leishmaniasis caused by L. braziliensis.

Stabilized Acoustic Levitation of Dense Materials Using a High-Powered Siren

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Gammell, P. M.; Croonquist, A.; Wang, T. G.

1982-01-01

Stabilized acoustic levitation and manipulation of dense (e.g., steel) objects of 1 cm diameter, using a high powered siren, was demonstrated in trials that investigated the harmonic content and spatial distribution of the acoustic field, as well as the effect of sample position and reflector geometries on the acoustic field. Although further optimization is possible, the most stable operation achieved is expected to be adequate for most containerless processing applications. Best stability was obtained with an open reflector system, using a flat lower reflector and a slightly concave upper one. Operation slightly below resonance enhances stability as this minimizes the second harmonic, which is suspected of being a particularly destabilizing influence.

CLINICAL APPLICATION OF RECOMBINANT ERYTHROPOIETIN IN BETA-THALASSAEMIA INTERMEDIA.

Science.gov (United States)

Asadov, Ch; Alimirzoyeva, Z; Hasanova, M; Mammadova, T; Shirinova, A

2016-06-01

Research objective is to study the efficacy of recombinant erythropoietin (epoetin alfa) as alternative method of treatment beta-thalassemia intermedia. Study involved 58 patients with beta-thalassemia intermedia (23 women and 35 men). In all observed patients was defined levels of hemoglobin (Hb), red blood cells (RBC), erythrocyte indexes (MCV, MCH, MCHC), hemoglobin fractions (HbA, HbA2, HbF), serum ferritin, serum erythropoietin before and after administrated rEPO. All patients received rEPO during 6 month at the dose - 10000 IU subcutaneously. The majority of patients - 39 (67%) had a good response to rEPO (increase in hemoglobin level more than 20 g/l); 16 patients (28%) had a mean response (increase in Hb 10 - 20 g/l); in 3 (5%) patients occurred poor response to rEPO therapy (increase in Hb intermedia patients there was a statistically significant change in the number of RBC, levels of HbF and sEPO. The evaluation of interdependence between the indices of the baseline sEPO and increased Hb values in patients after rEPO treatment revealed the presence of the reverse direct relationship (r=-0.67). Based on the results, it can be concluded that the use of rEPO in complex therapy of beta-thalassemia intermedia leads to increased levels of Hb and consequently reducing the need for blood transfusions, and accordingly expected to prevent severe complications of blood transfusion (alloimmunization, hypersplenism, iron overload, contamination transmissible infections) facilitating normal growth and development, and a better quality of life.

Composition, Cytotoxic and Antimicrobial Activities of Satureja intermedia C.A.Mey Essential Oil.

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Sharifi-Rad, Javad; Sharifi-Rad, Mehdi; Hoseini-Alfatemi, Seyedeh Mahsan; Iriti, Marcello; Sharifi-Rad, Majid; Sharifi-Rad, Marzieh

2015-08-03

In this study, the essential oil (EO) constituents from the aerial parts of Satureja intermedia C.A.Mey were detected by GC and GC/MS. The antimicrobial activity of EO on oral pathogens and its cytotoxicity to human cancer cells were determined by the microbroth dilution method and the crystal violet staining method, respectively. Thirty-nine compounds were identified and the main EO constituents were γ-terpinene (37.1%), thymol (30.2%), p-cymene (16.2%), limonene (3.9%), α-terpinene (3.3%), myrcene (2.5%), germacrene B (1.4%), elemicine (1.1%) and carvacrol (0.5%). The S. intermedia EO showed a concentration-dependent decrease in viability of Hep-G2 (hepatocellular carcinoma) and MCF-7 (breast adenocarcinoma) human cancer cell lines (p intermedia EO demonstrated slight antibacterial and antifungal activities against Streptococcus mutants, S. salivarius, Enterococcus faecalis, Staphylococcus aureus, Candida albicans and C. glabrata. Further preclinical studies are needed to assess the efficacy and safety of S. intermedia EO as a new promising anticancer agent.

Contribution of hly homologs to the hemolytic activity of Prevotella intermedia.

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Suzuki, Naoko; Fukamachi, Haruka; Arimoto, Takafumi; Yamamoto, Matsuo; Igarashi, Takeshi

2012-06-01

Prevotella intermedia is a periodontal pathogen that requires iron for its growth. Although this organism has hemolytic activity, the precise nature of its hemolytic substances and their associated hemolytic actions are yet to be fully determined. In the present study, we identified and characterized several putative hly genes in P. intermedia ATCC25611 which appear to encode hemolysins. Six hly genes (hlyA, B, C, D, E, and hlyI) of P. intermedia were identified by comparing their nucleotide sequences to those of known hly genes of Bacteroides fragilis NCTC9343. The hlyA-E, and hlyI genes were overexpressed individually in the non-hemolytic Escherichia coli strain JW5181 and examined its contribution to the hemolytic activity on sheep blood agar plates. E. coli cells expressing the hlyA and hlyI genes exhibited hemolytic activity under anaerobic conditions. On the other hand, only E. coli cells stably expressing the hlyA gene were able to lyse the red blood cells when cultured under aerobic conditions. In addition, expression of the hlyA and hlyI genes was significantly upregulated in the presence of red blood cells. Furthermore, we found that the growth of P. intermedia was similar in an iron-limited medium supplemented with either red blood cells or heme. Taken together, our results indicate that the hlyA and hlyI genes of P. intermedia encode putative hemolysins that appear to be involved in the lysis of red blood cells, and suggest that these hemolysins might play important roles in the iron-dependent growth of this organism. Copyright © 2012 Elsevier Ltd. All rights reserved.

Restriction-modification mediated barriers to exogenous DNA uptake and incorporation employed by Prevotella intermedia.

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Johnston, Christopher D; Skeete, Chelsey A; Fomenkov, Alexey; Roberts, Richard J; Rittling, Susan R

2017-01-01

Prevotella intermedia, a major periodontal pathogen, is increasingly implicated in human respiratory tract and cystic fibrosis lung infections. Nevertheless, the specific mechanisms employed by this pathogen remain only partially characterized and poorly understood, largely due to its total lack of genetic accessibility. Here, using Single Molecule, Real-Time (SMRT) genome and methylome sequencing, bisulfite sequencing, in addition to cloning and restriction analysis, we define the specific genetic barriers to exogenous DNA present in two of the most widespread laboratory strains, P. intermedia ATCC 25611 and P. intermedia Strain 17. We identified and characterized multiple restriction-modification (R-M) systems, some of which are considerably divergent between the two strains. We propose that these R-M systems are the root cause of the P. intermedia transformation barrier. Additionally, we note the presence of conserved Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) systems in both strains, which could provide a further barrier to exogenous DNA uptake and incorporation. This work will provide a valuable resource during the development of a genetic system for P. intermedia, which will be required for fundamental investigation of this organism's physiology, metabolism, and pathogenesis in human disease.

SYNCHROTRON X-RAY ABSORPTION-EDGE COMPUTED MICROTOMOGRAPHY IMAGING OF THALLIUM COMPARTMENTALIZATION IN IBERIS INTERMEDIA

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Thallium (TI) is an extremely toxic metal which, due to its similarities to K, is readily taken up by plants. Thallium is efficiently hyperaccumulated in Iberis intermedia as TI(I). Distribution and compartmentalization of TI in I. intermedia is highes...

Effect of estradiol on planktonic growth, coaggregation, and biofilm formation of the Prevotella intermedia group bacteria.

Science.gov (United States)

Fteita, Dareen; Könönen, Eija; Söderling, Eva; Gürsoy, Ulvi Kahraman

2014-06-01

Alterations in the quantity and quality of biofilms at gingival margin are considered to play a role in the initiation and development of pregnancy-related gingivitis. Prevotella intermedia sensu lato is able to consume estradiol, the major sex hormone secreted during pregnancy, in the absence of vitamin K. The aim of the study was to examine the effect of estradiol on the planktonic growth, coaggregation, polysaccharide production, and biofilm formation of the P. intermedia group bacteria, namely P. intermedia, Prevotella nigrescens, and Prevotella pallens. In all experiments, the type strain (ATCC) and a clinical strain (AHN) of P. intermedia, P. nigrescens, and P. pallens were incubated with the concentrations of 0, 30, 90, and 120 nmol/L of estradiol. Planktonic growth was assessed by means of the colony forming unit method, while coaggregation and biofilm formation were assessed by spectrophotometric methods. In the determination of protein and polysaccharide levels, the Bradford and phenol-sulfuric acid methods were used, respectively. P. pallens AHN 9283 and P. nigrescens ATCC 33563 increased their numbers at planktonic stage with increasing estradiol concentrations. In 48-h biofilm tests, elevated protein levels were found for both strains of P. intermedia, and the strains P. nigrescens ATCC 33563 and P. pallens AHN 9283 in the presence of estradiol. The P. intermedia strains also increased the levels of polysaccharide formation in the biofilm. Coaggregation of the P. intermedia group organisms with Fusobacterium nucleatum was enhanced only in P. intermedia AHN 8290. In conclusion, our in vitro experiments indicate that estradiol regulates planktonic growth, coaggregation, polysaccharide production, and biofilm formation characteristics of P. intermedia, P. nigrescens, and P. pallens differently. These results may, at least partly, explain the differences seen in their contribution to the pathogenesis of pregnancy-related gingivitis

Relative contribution of Prevotella intermedia and Pseudomonas aeruginosa to lung pathology in airways of patients with cystic fibrosis.

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Ulrich, Martina; Beer, Isabelle; Braitmaier, Peter; Dierkes, Michaela; Kummer, Florian; Krismer, Bernhard; Schumacher, Ulrike; Gräpler-Mainka, Ute; Riethmüller, Joachim; Jensen, Peter Ø; Bjarnsholt, Thomas; Høiby, Niels; Bellon, Gabriel; Döring, Gerd

2010-11-01

Patients with cystic fibrosis (CF) with Pseudomonas aeruginosa lung infections produce endobronchial mucus plugs allowing growth of obligate anaerobes including Prevotella spp. Whether obligate anaerobes contribute to the pathophysiology of CF lung disease is unknown. The virulence of Prevotella intermedia and Ps aeruginosa was investigated in vitro and in mice, antibodies against P intermedia in CF sera were assessed and a culture-independent detection method for P intermedia/P nigrescens in CF sputum was tested. P intermedia reached cell numbers of >10(5)->10(7) colony-forming units (CFU)/ml sputum. The majority of patients with CF (16/17; 94.1%) produced antibodies against two immunoreactive antigens of P intermedia. Culture supernatant fluids, collected from 10(9) P intermedia cells, were more cytotoxic to respiratory epithelial cells in vitro and inflammatory in mouse lungs than respective fluids from anaerobically grown Ps aeruginosa, while fluids from aerobically grown Ps aeruginosa had the highest cytotoxicity and inflammation. Both pathological effects were largely reduced when culture supernatant fluids from 10(7) cells of either species were used. P intermedia cells (∼10(6)CFU/lung) did not induce mortality in the agar beads lung infection mouse model, while Ps aeruginosa cells caused death in 30% of mice due to rapid multiplication. A P intermedia/P nigrescens-specific PNA probe was significantly more sensitive than culture-dependent diagnostic assays to detect these strict anaerobes. Ps aeruginosa and P intermedia become significantly virulent in vitro and in vivo when cell numbers exceed 10(8) CFU/lung.

Incidence of Prevotella intermedia and Prevotella nigrescens Carriage among Family Members with Subclinical Periodontal Disease

Science.gov (United States)

Fukui, Katsuhito; Kato, Naoki; Kato, Haru; Watanabe, Kunitomo; Tatematsu, Norichika

1999-01-01

We established a typing system for Prevotella intermedia and Prevotella nigrescens using the combination of PCR ribotyping and arbitrarily primed PCR (AP-PCR) fingerprinting and applied this system to the study of intrafamilial incidence of these species in the oral cavity. PCR ribotyping followed by subtyping by AP-PCR fingerprinting was applied to each type strain of P. intermedia and P. nigrescens and 54 isolates (32 isolates of P. intermedia and 24 isolates of P. nigrescens) from extraoral infections, resulting in an excellent discriminatory power (discrimination index, 0.99) for both species. A total of 18 subjects from six families, with the subjects from each family comprising the mother, the father, and a child who had subclinical early-stage to moderate adult periodontitis or simple gingivitis and who carried P. intermedia or P. nigrescens, or both, were enrolled in the study of intrafamilial carriage. When 20 colonies per specimen of subgingival plaque, if available, were picked from primary culture, 115 P. intermedia and 178 P. nigrescens isolates were recovered from the 18 subjects. Among the subjects studied, family members shared the same subtype strain(s) but non-family members did not. Multiple subtypes were found in 8 (57%) of the 14 P. nigrescens-positive subjects but in only 3 (27%) of the 11 P. intermedia-positive subjects; the difference was, however, not statistically significant (P = 0.14). These results suggest that the combination of PCR ribotyping and AP-PCR fingerprinting is well suited for the epidemiological study of P. intermedia and P. nigrescens and that each family seems to carry a distinct subtype(s) of these species. PMID:10488167

Prevalence of Pulmonary Hypertension in Patients with Thalassemia Intermedia in 2009: a single center's experience.

Science.gov (United States)

Moghaddam, Hassan Mottaghi; Badiei, Zahra; Eftekhari, Kambiz; Shakeri, Reza; Farhangi, Hamid

2015-07-01

There are various clinical symptoms of thalassemia intermedia, and they lie roughly between those of major and minor forms of the disease. Patients with thalassemia intermedia occasionally require blood transfusions. This renders them susceptible to pulmonary arterial hypertension (PAH) syndrome, which is one of the most significant complications in patients with thalassemia intermedia. PAH is more common in in thalassemia intermedia than in thalassemia major, and it may cause cardiac complications in patients who are older than 30. The objective of this study was to estimate the prevalence of PAH in thalassemia intermedia patients so that they can be referred expeditiously for treatment, thereby preventing the complications that occur later. This cross sectional study was conducted under the supervision of hematology department of Mashhad Medical University. Forty-one patients with thalassemia intermedia were examined at the Sarvar Thalassemia and Hemophilia Clinic of Mashhad. Electrocardiography, chest radiography, and echocardiography tests were performed for all of the patients by the same pediatric cardiologist. The data were processed by SPSS software, version 11.5, and the results were analyzed using chi-squared, Student's t, and Mann-Whitney tests. The mean age of the patients was 21.93±8.34. They had been under pediatric heart specialists' constant examination and treatment since their childhood when they were diagnosed with TI, and continue to receive regular follow-up care. The prevalence of pulmonary hypertension was 24% in our study population. In patients with thalassemia intermedia, the left ventricular (LV) mass indices were about 3-5 times higher than would be expected in a normal population. Patients with higher LV mass indices have a greater risk of developing pulmonary hypertension, and those with serum ferritin levels below 1000 ng/ml are less susceptible to diastolic dysfunction. Pulmonary hypertension is common in patients with thalassemia

Composition, Cytotoxic and Antimicrobial Activities of Satureja intermedia C.A.Mey Essential Oil

Directory of Open Access Journals (Sweden)

Javad Sharifi-Rad

2015-08-01

Full Text Available In this study, the essential oil (EO constituents from the aerial parts of Satureja intermedia C.A.Mey were detected by GC and GC/MS. The antimicrobial activity of EO on oral pathogens and its cytotoxicity to human cancer cells were determined by the microbroth dilution method and the crystal violet staining method, respectively. Thirty-nine compounds were identified and the main EO constituents were γ-terpinene (37.1%, thymol (30.2%, p-cymene (16.2%, limonene (3.9%, α-terpinene (3.3%, myrcene (2.5%, germacrene B (1.4%, elemicine (1.1% and carvacrol (0.5%. The S. intermedia EO showed a concentration-dependent decrease in viability of Hep-G2 (hepatocellular carcinoma and MCF-7 (breast adenocarcinoma human cancer cell lines (p < 0.05. Antimicrobial screening of S. intermedia EO demonstrated slight antibacterial and antifungal activities against Streptococcus mutants, S. salivarius, Enterococcus faecalis, Staphylococcus aureus, Candida albicans and C. glabrata. Further preclinical studies are needed to assess the efficacy and safety of S. intermedia EO as a new promising anticancer agent.

Experimental weed control of Najas marina ssp. intermedia and Elodea nuttallii in lakes using biodegradable jute matting

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Markus A. Hoffmann

2013-10-01

Full Text Available The use of jute matting in managing the invasive aquatic macrophyte species Elodea nuttallii (Planch. H. St. John and Najas marina ssp. intermedia (Wolfg. ex Gorski Casper (Najas intermedia was studied in laboratory experiments and field trials. Four German lakes with predominant population of Najas intermedia or Elodea nuttalli were chosen for the experiment and areas between 150 and 300 m² were covered with jute textile. The effect of the matting on the growth of invasive and non-invasive macrophytes was determined through comparison with control transects. Biodegradable jute matting successfully suppressed the invasive macrophyte Najas intermedia and significantly reduced the growth of Elodea nuttalli in lakes. The results indicate that the capability of the matting to inhibit the growth of Elodea nuttallii and Najas intermedia depends on the mesh size of the jute weaving and that environmental conditions can affect its efficiency. Various indigenous species like Charales or Potamogeton pusillus L. were able to grow through the jute fabric and populate the treated areas. Until the end of the vegetation period, none of the invasive species were able to penetrate the covering and establish a stable population; in fact, in the subsequent year the jute matting affected only the spread of Najas intermedia. Jute matting proved to be an easy-to-use and cheap method to control the growth of Elodea nuttallii and Najas intermedia.

Analisis Strategi Pemasaran Internasional Pada Produk Revoluzio (Studi Pada PT Beon Intermedia)

OpenAIRE

Aisyah, Nugrahanti Khairani; Mawardi, M. Kholid

2017-01-01

PT Beon Intermedia is company engaged in the field of ICT (information and communication technology. Based on preliminary observations conducted by researchers in the field, Revoluzio is an innovative e-business to implement automation in digital marketing. This product is an innovative e-business the most and become the only one, both Indonesia and abroad, so the company should also establish appropriate strategy for the International market. The aim of this study was to describe the changes...

In vitro Resistance Testing of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to Triclosan.

Science.gov (United States)

Farsi, Deema; Tanner, Anne

2016-04-01

To determine the sensitivity of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to triclosan, and determine if these bacteria develop resistance to triclosan upon prolonged exposure. Susceptibility to triclosan was tested against three periodontal pathogens P. gingivalis, P. intermedia, and T. forsythia. Escherichia coli strains sensitive and resistant to triclosan were used as biological controls to confirm the efficacy of triclosan in the assays. Agar plates were prepared locally with vitamin K and hemin-supplemented medium. Porphyromonas gingivalis and P. intermedia did not grow on plates containing ≥ 2 μg/ml triclosan, while T. forsythia did not grow on ≥ 1.66 μg/ml. Colonies of P. intermedia resistant to triclosan developed after prolonged incubation at 2 μg/ml, but this resistance disappeared during subculture in the absence of triclosan. No significant resistance to triclosan was detected for these species. Dental products containing triclosan can be beneficial in controlling periodontal disease.

A high-powered siren for stable acoustic levitation of dense materials in the earth's gravity

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Gammel, Paul M.; Croonquist, Arvid P.; Wang, Taylor G.

1988-01-01

Levitation of large dense samples (e.g., 1-cm diameter steel balls) has been performed in a 1-g environment. A siren was used to study the effects of reflector geometry and variable-frequency operation in order to attain stable acoustic positioning. The harmonic content and spatial distribution of the acoustic field have been investigated. The best stability was obtained with an open reflector system, using a flat lower reflector and a slightly concave upper reflector while operating at a frequency slightly below resonance.

Comparative genome analysis of Prevotella intermedia strain isolated from infected root canal reveals features related to pathogenicity and adaptation.

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Ruan, Yunfeng; Shen, Lu; Zou, Yan; Qi, Zhengnan; Yin, Jun; Jiang, Jie; Guo, Liang; He, Lin; Chen, Zijiang; Tang, Zisheng; Qin, Shengying

2015-02-25

Many species of the genus Prevotella are pathogens that cause oral diseases. Prevotella intermedia is known to cause various oral disorders e.g. periodontal disease, periapical periodontitis and noma as well as colonize in the respiratory tract and be associated with cystic fibrosis and chronic bronchitis. It is of clinical significance to identify the main drive of its various adaptation and pathogenicity. In order to explore the intra-species genetic differences among strains of Prevotella intermedia of different niches, we isolated a strain Prevotella intermedia ZT from the infected root canal of a Chinese patient with periapical periodontitis and gained a draft genome sequence. We annotated the genome and compared it with the genomes of other taxa in the genus Prevotella. The raw data set, consisting of approximately 65X-coverage reads, was trimmed and assembled into contigs from which 2165 ORFs were predicted. The comparison of the Prevotella intermedia ZT genome sequence with the published genome sequence of Prevotella intermedia 17 and Prevotella intermedia ATCC25611 revealed that ~14% of the genes were strain-specific. The Preveotella intermedia strains share a set of conserved genes contributing to its adaptation and pathogenic and possess strain-specific genes especially those involved in adhesion and secreting bacteriocin. The Prevotella intermedia ZT shares similar gene content with other taxa of genus Prevotella. The genomes of the genus Prevotella is highly dynamic with relative conserved parts: on average, about half of the genes in one Prevotella genome were not included in another genome of the different Prevotella species. The degree of conservation varied with different pathways: the ability of amino acid biosynthesis varied greatly with species but the pathway of cell wall components biosynthesis were nearly constant. Phylogenetic tree shows that the taxa from different niches are scarcely distributed among clades. Prevotella intermedia ZT

Pigment Production by the Edible Filamentous Fungus Neurospora Intermedia

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Rebecca Gmoser

2018-02-01

Full Text Available The production of pigments by edible filamentous fungi is gaining attention as a result of the increased interest in natural sources with added functionality in the food, feed, cosmetic, pharmaceutical and textile industries. The filamentous fungus Neurospora intermedia, used for production of the Indonesian food “oncom”, is one potential source of pigments. The objective of the study was to evaluate the fungus’ pigment production. The joint effect from different factors (carbon and nitrogen source, ZnCl2, MgCl2 and MnCl2 on pigment production by N. intermedia is reported for the first time. The scale-up to 4.5 L bubble column bioreactors was also performed to investigate the effect of pH and aeration. Pigment production of the fungus was successfully manipulated by varying several factors. The results showed that the formation of pigments was strongly influenced by light, carbon, pH, the co-factor Zn2+ and first- to fourth-order interactions between factors. The highest pigmentation (1.19 ± 0.08 mg carotenoids/g dry weight biomass was achieved in a bubble column reactor. This study provides important insights into pigmentation of this biotechnologically important fungus and lays a foundation for future utilizations of N. intermedia for pigment production.

Las organizaciones intermedias en el campo de la responsabilidad social empresarial

OpenAIRE

Abelenda Casalet, Lucía

2010-01-01

Se presenta un estudio sobre un tipo particular de organizaciones de la sociedad civil, las organizaciones intermedias. Focalizamos el estudio en las organizaciones intermedias que se desempeñan en el campo de la Responsabilidad Social Empresarial. La investigación está orientada a la búsqueda de rasgos que puedan definir la acción de estas organizaciones y su capacidad de innovación social en un campo particular. En el desarrollo de la investigación se jerarquizó el nivel micro, relacionado ...

Hearing the music of the primes: auditory complementarity and the siren song of zeta

International Nuclear Information System (INIS)

Berry, M V

2012-01-01

A counting function for the primes can be rendered as a sound signal whose harmonies, spanning the gamut of musical notes, are the Riemann zeros. But the individual primes cannot be discriminated as singularities in this ‘music’, because the intervals between them are too short. Conversely, if the prime singularities are detected as a series of clicks, the Riemann zeros correspond to frequencies too low to be heard. The sound generated by the Riemann zeta function itself is very different: a rising siren howl, which can be understood in detail from the Riemann–Siegel formula. (fast track communication)

Draft genome sequence of a Kluyvera intermedia isolate from a patient with a pancreatic abscess.

Science.gov (United States)

Thele, Roland; Gumpert, Heidi; Christensen, Louise B; Worning, Peder; Schønning, Kristian; Westh, Henrik; Hansen, Thomas A

2017-09-01

The genus Kluyvera comprises potential pathogens that can cause many infections. This study reports a Kluyvera intermedia strain (FOSA7093) from a pancreatic cyst specimen from a long-term hospitalised patient. Whole-genome sequencing (WGS) of the K. intermedia isolate was performed and the strain was reported as sensitive to Danish-registered antibiotics although it had a fosA-like gene in the genome. There were nine contigs that aligned to a plasmid, and these contigs contained several heavy metal resistance gene homologues. Furthermore, a prophage was discovered in the genome. WGS represents an efficient tool for monitoring Kluyvera spp. and its role as a reservoir of multidrug resistance. Therefore, this susceptible K. intermedia genome has many characteristics that allow comparison of resistant K. intermedia that might be discovered in the future. Copyright © 2017 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

Cloning, Codon Optimization, and Expression of Yersinia intermedia Phytase Gene in E. coli.

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Mirzaei, Maryam; Saffar, Behnaz; Shareghi, Behzad

2016-06-01

Phytate is an anti-nutritional factor in plants, which catches the most phosphorus contents and some vital minerals. Therefore, Phytase is added mainly as an additive to the monogastric animals' foods to hydrolyze phytate and increase absorption of phosphorus. Y. intermedia phytase is a new phytase with special characteristics such as high specific activity, pH stability, and thermostability. Our aim was to clone, express, and characterizea codon optimized Y. intermedia phytase gene in E. coli . The Y. intermedia phytase gene was optimized according to the codon usage in E. coli . The sequence was synthesized and sub-cloned in pET-22b (+) vector and transformed into E. coli Bl21 (DE3). The protein was expressed in the presence of IPTG at a final concentration of 1 mM at 30°C. The purification of recombinant protein was performed by Ni 2+ affinity chromatography. Phytase activity and stability were determined in various pH and temperatures. The codon optimized Y. intermedia phytase gene was sub-cloned successfully.The expression was confirmed by SDS-PAGE and Western blot analysis. The recombinant enzyme (approximately 45 kDa) was purified. Specific activity of enzyme was 3849 (U.mg -1 ) with optimal pH 5 and optimal temperature of 55°C. Thermostability (80°C for 15 min) and pH stability (3-6) of the enzyme were 56 and more than 80%, respectively. The results of the expression and enzyme characterization revealed that the optimized Y. intermedia phytase gene has a good potential to be produced commercially andto be applied in animals' foodsindustry.

Taxonomic study on Japanese Salvia (Lamiaceae): Phylogenetic position of S. akiensis, and polyphyletic nature of S. lutescens var. intermedia.

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Takano, Atsuko

2017-01-01

Both Salvia akiensis and S. lutescens (Lamiaceae) are endemic to Japan. Salvia akiensis was recently described in 2014 in the Chugoku (= SW Honshu) region, and each four varieties of S. lutescens distributed allopatrically. Among varieties in S. lutescens , var. intermedia show a disjunctive distribution in the Kanto (=E Honshu) and Kinki (= W Honshu) regions. Recent field studies of S. lutescens var. intermedia revealed several morphological differences between the Kanto and Kinki populations. Here, I evaluated these differences among Salvia lutescens var. intermedia and its allies with morphological analysis and molecular phylogenetic analyses of nuclear ribosomal DNA (internal and external transcribed spacer regions) and plastid DNA ( ycf1-rps15 spacer, rbcL , and trnL-F ) sequences. Both morphological analysis and molecular phylogenetic analyses showed that S. lutescens var. intermedia from the Kinki region and var. lutescens were closely related to each other. However, var. intermedia from the Kanto region exhibited an association with S. lutescens var. crenata and var. stolonifera, which also grew in eastern Japan, rather than var. intermedia in the Kinki region. These results indicated that S. lutescens var. intermedia is not a taxon with a disjunctive distribution, but a combination of two or more allopatric taxa. Present study also suggested that S. akiensis was most closely related to S. omerocalyx .

Equine pituitary pars intermedia dysfunction: current understanding and recommendations from the Australian and New Zealand Equine Endocrine Group.

Science.gov (United States)

Secombe, C J; Bailey, S R; de Laat, M A; Hughes, K J; Stewart, A S; Sonis, J M; Tan, Rhh

2018-06-03

The purpose of this article is to provide a review of the current knowledge and opinions about the epidemiology, clinical findings (including sequelae), diagnosis, treatment and monitoring of equine pituitary pars intermedia dysfunction, particularly in the Australian context. This information and the recommendations provided will assist practitioners in making informed decisions regarding the diagnosis and management of this disorder. © 2018 Australian Veterinary Association.

Anti-inflammatory effect of extract and fractions from the leaves of Byrsonima intermedia A. Juss. in rats.

Science.gov (United States)

Moreira, Lucimara Q; Vilela, Fabiana C; Orlandi, Lidiane; Dias, Danielle F; Santos, Ana Laura A; da Silva, Marcelo A; Paiva, Renato; Alves-da-Silva, Geraldo; Giusti-Paiva, Alexandre

2011-11-18

Byrsonima intermedia is commonly used for its antiseptic, antimicrobial, and anti-inflammatory properties in the treatment of diarrhea and dysentery in Brazilian folk medicine. The purpose of this study was to examine the anti-inflammatory activity of the aqueous extract and fractions of Byrsonima intermedia leaves. Rats with carrageenan-induced paw edema and fibrovascular tissue growth, which was induced by subcutaneous implantation of a cotton pellet, were used as acute and chronic animal models of inflammation to investigate the anti-inflammatory effects of the aqueous extract and the individual ethyl acetate (EtOAc) and aqueous fractions of Byrsonima intermedia and catechin. High-performance liquid chromatography (HPLC) was used to determine the fingerprint chromatogram of the aqueous extract and fractions of Byrsonima intermedia. The crude aqueous extract at test doses of 30-300 mg/kg p.o. clearly demonstrated anti-inflammatory effects by reducing carrageenan-induced paw edema, as did the ethyl acetate (100mg/kg) and aqueous fractions (30-100mg/kg). In the chronic inflammation rat animal model with fibrovascular tissue growth, the aqueous extract of Byrsonima intermedia (BiAE) at doses of 30-300 mg/kg and the individual EtOAc and aqueous fractions at doses of 30-100mg/kg and catechin significantly reduced the formation of granulomatous tissue. The presence of catechin and phenolic compounds in the extract and fractions of Byrsonima intermedia was confirmed using HPLC. BiAE and the individual EtOAc and aqueous fractions of Byrsonima intermedia exhibited chronic and acute anti-inflammatory efficacy in rats, which supports previous claims of its use in traditional medicine. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Expression Profiles of TGF-β and TLR Pathways in Porphyromonas gingivalis and Prevotella intermedia Challenged Osteoblasts.

Science.gov (United States)

Aydin, Kubra; Ekinci, Fatma Yesim; Korachi, May

2015-04-01

The presence of certain oral pathogens at implant sites can hinder the osseointegration process. However, it is unclear how and by what microorganisms it happens. This study investigated whether the presence of oral pathogens of Porphyromonas gingivalis and Prevotella intermedia individually, play a role in the failure of bone formation by determining the expression profiles of Transforming Growth Factor Beta (TGF-β/Bone Morphogenic Protein (BMP) and Toll-Like Receptor (TLR) pathways in challenged osteoblasts. Cell viability of P. gingivalis and P. intermedia challenged osteoblasts were determined by WST assay. Changes in osteoblast morphology and inhibition of mineralization were observed by Scanning Electron Microscopy (SEM) and Von Kossa staining, respectively. Expression of TGF-β and TLR pathway genes on challenged cells were identified by RT profiler array. Both P. gingivalis and P. intermedia challenges resulted in reduced viability and mineralization of osteoblasts. Viability was reduced to 56.8% (P. gingivalis) and 52.75% (P. intermedia) at 1000 multiplicity. Amongst 48 genes examined, expressions of BMPER, SMAD1, IL8 and NFRKB were found to be highly upregulated by both bacterial challenges (Fold Change > 4). P. gingivalis and P. intermedia could play a role in implant failure by changing the expression profiles of genes related to bone formation and resorption.

Formation of Lignans(-)-Secoisolariciresinol and (-)-Matairesinol with Forsythia intermedia Cell-Free Extracts

Science.gov (United States)

Umezawa, Toshiaki; Davin, Laurence B.; Lewis, Norman G.

1991-01-01

In vivo labeling experiments of Forsythia intermedia plant tissue with [8-(C-14)]- and [9,9-(2)H2,OC(2)H3]coniferyl alcohols revealed that the lignans, (-)-secoisolariciresinol and (-)-matairesinol, were derived from two coniferyl alcohol molecules; no evidence for the formation of the corresponding (+)-enantiomers was found. Administration of (+/-)-[Ar-(H-3)] secoisolariciresinols to excised shoots of F.intermedia resulted in a significant conversion into (-)-matairesinol; again, the (+)-antipode was not detected. Experiments using cell-free extracts of F.intermedia confirmed and extended these findings. In the presence of NAD(P)H and H2O2, the cell-free extracts catalyzed the formation of (-)- secoisolariciresinol, with either [8-(C-14)]- or [9,9-(2)H2,OC(2)H3]coniferyl alcohols as substrates. The (+)- enantiomer was not formed. Finally, when either (-)-[Ar-(H-3)] or (+/-)-[Ar-(H-2)]secoisolariciresinols were used as substrates, in the presence of NAD(P), only (-)- and not (+)-matairesinol formation occurred. The other antipode, (+)-secoisolariciresinol, did not serve as a substrate for the formation of either (+)- or (-)-matairesinol. Thus, in F.intermedia, the formation of the lignan, (-)-secoisolariciresinol, occurs under strict stereochemical control, in a reaction or reactions requiring NAD(P)H and H2O2 as cofactors. This stereoselectivity is retained in the subsequent conversion into (-)-matairesinol, since (+)-secoisolariciresinol is not a substrate. These are the first two enzymes to be discovered in lignan formation.

A comparison of heart function and arrhythmia in clinically asymptomatic patients with beta thalassemia intermedia and beta thalassemia major.

Science.gov (United States)

Amoozgar, Hamid; Zeighami, Samaneh; Haghpanah, Sezaneh; Karimi, Mehran

2017-01-01

The goal of this study was to compare heart function and arrhythmia in clinically asymptomatic patients with beta thalassemia intermedia and beta thalassemia major. In this cross-sectional study, 60 patients with beta thalassemia major and 60 patients with beta thalassemia intermedia who had clinically no symptoms of arrhythmia and clinically normal heart function were evaluated using 24-hour ambulatory electrocardiogram monitoring and echocardiography. For data analysis SPSS ver.20 software was used. A P-value of less than 0.05 was considered statistically significant. The mean age of the beta thalassemia intermedia patients was 24.18 ± 7.9 years and the mean age in beta thalassemia major was 24.38 ± 7.7 years (P>0.05). Premature atrial contractions (PACs) were observed in 14 (23.3%) patients with beta thalassemia intermedia and in 22 (36.6%) beta thalassemia major patients. Premature ventricular contractions (PVCs) were detected in 8 (13.3%) patients in the beta thalassemia intermediate group and 16 (26.6) patients in the beta thalassemia major group, respectively. The left ventricular diastolic dimension, end-diastolic volume, and stroke volume were significantly higher in beta thalassemia intermedia group (Pintermedia group. Both atrial and ventricular arrhythmias were more common in the beta thalassemia major group. Higher end-diastolic volume and stroke volume were detected in the beta thalassemia intermedia group. Pulmonary acceleration time was lower in the beta thalassemia intermedia group, which can be an indicator of higher pulmonary pressure.

Irradiation for progressive paraparesis in β-thalassemia intermedia

International Nuclear Information System (INIS)

Wilson, J.J.; Hill, M.E.; Crookston, J.H.

1980-01-01

This article describes the case history of a patient suffering from β-thalassemia intermedia. The patient was treated with radiation to the thoraic spine (1500 rad in 5 days) and his condition improved. Subsequent tomography indicated the success of this type of irradiation without recourse to laminectomy. (T.I.)

The mass remote sensing image data management based on Oracle InterMedia

Science.gov (United States)

Zhao, Xi'an; Shi, Shaowei

2013-07-01

With the development of remote sensing technology, getting the image data more and more, how to apply and manage the mass image data safely and efficiently has become an urgent problem to be solved. According to the methods and characteristics of the mass remote sensing image data management and application, this paper puts forward to a new method that takes Oracle Call Interface and Oracle InterMedia to store the image data, and then takes this component to realize the system function modules. Finally, it successfully takes the VC and Oracle InterMedia component to realize the image data storage and management.

Interpain A, a cysteine proteinase from Prevotella intermedia, inhibits complement by degrading complement factor C3.

Directory of Open Access Journals (Sweden)

Michal Potempa

2009-02-01

Full Text Available Periodontitis is an inflammatory disease of the supporting structures of the teeth caused by, among other pathogens, Prevotella intermedia. Many strains of P. intermedia are resistant to killing by the human complement system, which is present at up to 70% of serum concentration in gingival crevicular fluid. Incubation of human serum with recombinant cysteine protease of P. intermedia (interpain A resulted in a drastic decrease in bactericidal activity of the serum. Furthermore, a clinical strain 59 expressing interpain A was more serum-resistant than another clinical strain 57, which did not express interpain A, as determined by Western blotting. Moreover, in the presence of the cysteine protease inhibitor E64, the killing of strain 59 by human serum was enhanced. Importantly, we found that the majority of P. intermedia strains isolated from chronic and aggressive periodontitis carry and express the interpain A gene. The protective effect of interpain A against serum bactericidal activity was found to be attributable to its ability to inhibit all three complement pathways through the efficient degradation of the alpha-chain of C3 -- the major complement factor common to all three pathways. P. intermedia has been known to co-aggregate with P. gingivalis, which produce gingipains to efficiently degrade complement factors. Here, interpain A was found to have a synergistic effect with gingipains on complement degradation. In addition, interpain A was able to activate the C1 complex in serum, causing deposition of C1q on inert and bacterial surfaces, which may be important at initial stages of infection when local inflammatory reaction may be beneficial for a pathogen. Taken together, the newly characterized interpain A proteinase appears to be an important virulence factor of P. intermedia.

Effect of quercetin on the production of nitric oxide in murine macrophages stimulated with lipopolysaccharide from Prevotella intermedia.

Science.gov (United States)

Cho, Yun-Jung; Kim, Sung-Jo

2013-08-01

Nitric oxide (NO) is a short-lived bioactive molecule that is known to play an important role in the pathogenesis of periodontal disease. In the current study, we investigated the effect of the flavonoid quercetin on the production of NO in murine macrophages activated with lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen related to inflammatory periodontal disease, and tried to elucidate the underlying mechanisms of action. LPS was isolated from P. intermedia ATCC 25611 cells by the standard hot phenol-water method. The concentration of NO in cell culture supernatants was determined by measuring the accumulation of nitrite. Inducible NO synthase (iNOS) and heme oxygenase-1 (HO-1) protein expression, phosphorylation of c-Jun N-terminal kinase (JNK) and p38, inhibitory κB (IκB)-α degradation, and signal transducer and activator of transcription 1 (STAT1) phosphorylation were analyzed via immunoblotting. Quercetin significantly attenuated iNOS-derived NO production in RAW246.7 cells activated by P. intermedia LPS. In addition, quercetin induced HO-1 protein expression in cells activated with P. intermedia LPS. Tin protoporphyrin IX (SnPP), a competitive inhibitor of HO-1, abolished the inhibitory effect of quercetin on LPS-induced NO production. Quercetin did not affect the phosphorylation of JNK and p38 induced by P. intermedia LPS. The degradation of IκB-α induced by P. intermedia LPS was inhibited when the cells were treated with quercetin. Quercetin also inhibited LPS-induced STAT1 signaling. Quercetin significantly inhibits iNOS-derived NO production in murine macrophages activated by P. intermedia LPS via anti-inflammatory HO-1 induction and inhibition of the nuclear factor-κB and STAT1 signaling pathways. Our study suggests that quercetin may contribute to the modulation of host-destructive responses mediated by NO and appears to have potential as a novel therapeutic agent for treating inflammatory periodontal disease.

Effect of azithromycin on Prevotella intermedia lipopolysaccharide-induced production of interleukin-6 in murine macrophages.

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Choi, Eun-Young; Jin, Ji-Young; Choi, Jeom-Il; Choi, In Soon; Kim, Sung-Jo

2014-04-15

Interleukin-6 (IL-6) is a key proinflammatory cytokine which plays a central role in the pathogenesis of periodontal disease. Host modulatory agents targeting at inhibiting IL-6, therefore, appear to be beneficial in slowing the progression of periodontal disease and potentially reducing destructive aspects of the host response. The present study was designed to investigate the effect of the macrolide antibiotic azithromycin on IL-6 generation in murine macrophages treated with lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in inflammatory periodontal disease, and its mechanisms of action. Azithromycin significantly suppressed IL-6 production as well as its mRNA expression in P. intermedia LPS-activated RAW264.7 cells. LPS-induced activation of JNK and p38 was not affected by azithromycin treatment. Azithromycin failed to prevent P. intermedia LPS from degrading IκB-α. Instead, azithromycin significantly diminished nuclear translocation and DNA binding activity of NF-κB p50 subunit induced with LPS. Azithromycin inhibited P. intermedia LPS-induced STAT1 and STAT3 phosphorylation. In addition, azithromycin up-regulated the mRNA level of SOCS1 in cells treated with LPS. In conclusion, azithromycin significantly attenuated P. intermedia LPS-induced production of IL-6 in murine macrophages via inhibition of NF-κB, STAT1 and STAT3 activation, which is possibly related to the activation of SOCS1 signaling. Further in vivo studies are required to better evaluate the potential of azithromycin in the treatment of periodontal disease. Copyright © 2014 Elsevier B.V. All rights reserved.

Circaea x intermedia Ehrh. (Klein heksenkruid) een tuinplant?

NARCIS (Netherlands)

Beringen, Ruud

2001-01-01

Circaea x intermedia, a very rare taxon in the Netherlands, was found on a new locality on the edge of a woodland near the outskirts of the town of Ede. Growing conditions on the site are not suitable for both parental species ( C. alpina and C. lutetiana). Therefore it is most unlikely the hybrid

Effect of nitric oxide-releasing derivative of indomethacin on Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.

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Choe, So-Hui; Choi, Eun-Young; Hyeon, Jin-Yi; Choi, In Soon; Kim, Sung-Jo

2017-10-14

The purpose of this study was to investigate the influences of NCX 2121, a nitric oxide (NO)-releasing derivative of indomethacin, upon the generation of proinflammatory mediators using murine macrophages activated by lipopolysaccharide (LPS) isolated from Prevotella intermedia, which is one of the pathogens implicated in periodontal diseases. Inducible NO synthase (iNOS)-derived NO, IL-1β and IL-6 as well as their relevant mRNA were significantly attenuated by NCX 2121 in RAW264.7 cells activated by P. intermedia LPS. NCX 2121 was much more effective than the parental compound indomethacin in reducing these proinflammatory mediators. NCX 2121 triggered induction of heme oxygenase-1 (HO-1) in cells exposed to P. intermedia LPS, and its inhibitory influence upon P. intermedia LPS-elicited NO generation was notably blocked by SnPP treatment. NCX 2121 attenuated NF-κB-dependent SEAP release induced by P. intermedia LPS. NCX 2121 did not display inhibitory action towards IκB-α degradation triggered by LPS. Instead, it significantly diminished nuclear translocation as well as DNA-binding action of NF-κB p50 subunit elicited by P. intermedia LPS. Further, NCX 2121 significantly up-regulated SOCS1 mRNA expression in cells challenged with P. intermedia LPS. In summary, NCX 2121 down-regulates P. intermedia LPS-elicited generation of NO, IL-1β and IL-6 in murine macrophages in a mechanism that involves anti-inflammatory HO-1 induction as well as decrement of NF-κB activation, which may be associated with SOCS1 expression. NCX 2121 may have potential benefits as a host immunomodulatory agent for the therapy of periodontal disease. Copyright © 2017 Elsevier Inc. All rights reserved.

Las organizaciones intermedias en los procesos de innovación en México

OpenAIRE

Pérez Hernández, Maria del Pilar Monserrat

2016-01-01

Resumen: La actividad innovadora depende de los vínculos o mediación que exista entre los actores que generan la tecnología y el conocimiento y los usuarios finales de una y otro; este papel lo cumplen diversas organizaciones intermedias (OI), las cuales traducen las necesidades de los usuarios de tecnologías en desarrollo. Este trabajo analiza el papel de dichas organizaciones intermedias en los procesos de innovación en México. Así, se distingue que existen tres vacíos estructurales: en la ...

Estudo da competência vetorial de Lutzomyia intermedia (Lutz & Neiva, 1912 para Leishmania (Viannia braziliensis, Vianna, 1911 Study of the vectorial competence of Lutzomyia intermedia (Lutz & Neiva, 1912 to Leishmania (Viannia braziliensis, Vianna, 1911

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Antonio Carlos da Silva

2001-04-01

Full Text Available Estudou-se a competência vetorial de Lutzomyia intermedia (Diptera: Psychodidae do Vale do Ribeira (SP para estirpes de Leishmania (Viannia braziliensis (Kinetoplastida: Trypanosomatidae, mediante pesquisa de infectividade natural; exposições de fêmeas silvestres e colonizadas (F1 às lesões de hamsters experimentalmente infectados e testes de transmissão via picada. A infectividade natural e os testes de transmissão revelaram-se negativos e, nas exposições, foram obtidas positividades de 74% (123+/166 dissecados e 70% (115+/164 dissecados para fêmeas silvestres e colonizadas respectivamente, e o desenvolvimento das formas evolutivas compatíveis com o modelo Peripilaria. A suscetibilidade às estirpes testadas associada aos indicadores epidemiológicos concorrem para a suspeita do papel vetorial de Lutzomyia intermedia na região estudada.This paper investigated the vectorial competence of Lutzomyia intermedia (Diptera: Psychodidae in Vale do Ribeira (SP to strains of Leishmania (Viannia braziliensis (Kinetoplastida: Trypanosomatidae, by means of a search for natural infection; exposure of wild and colonized females (F1 to the lesions of experimentally infected hamsters and transmission tests by bite. The natural infection and the transmission tests were negative. In the exposures of Lu. intermedia to infected lesions we found rates of 74% (123+/166 dissected and 70% (115+/164 dissected for the wild and colonized females respectively. The development of the parasites was compatible with the development model of Peripilaria. The susceptibility of the tested strains associated with the epidemiological indicators contribute to the vectorial role suspicion of Lutzomyia intermedia in the studied region.

Identification and functional analysis of the gene cluster for fructan utilization in Prevotella intermedia.

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Fuse, Haruka; Fukamachi, Haruka; Inoue, Mitsuko; Igarashi, Takeshi

2013-02-25

Fructanase enzymes hydrolyze the β-2,6 and β-2,1 linkages of levan and inulin fructans, respectively. We analyzed the influence of fructan on the growth of Prevotella intermedia. The growth of P. intermedia was enhanced by addition of inulin, implying that P. intermedia could also use inulin. Based on this finding, we identified and analyzed the genes encoding a putative fructanase (FruA), sugar transporter (FruB), and fructokinase (FruK) in the genome of strain ATCC25611. Transcript analysis by RT-PCR showed that the fruABK genes were co-transcribed as a single mRNA and semi-quantitative analysis confirmed that the fruA gene was induced in response to fructose and inulin. Recombinant FruA and FruK were purified and characterized biochemically. FruA strongly hydrolyzed inulin, with slight degradation of levan via an exo-type mechanism, revealing that FruA is an exo-β-d-fructanase. FruK converted fructose to fructose-6-phosphate in the presence of ATP, confirming that FruK is an ATP-dependent fructokinase. These results suggest that P. intermedia can utilize fructan as a carbon source for growth, and that the fructanase, sugar transporter, and fructokinase proteins we identified are involved in this fructan utilization. Copyright © 2012 Elsevier B.V. All rights reserved.

Incomplete paraplegia caused by extramedullary hematopoiesis in a patient with thalassemia intermedia.

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Hisamud-Din, Nurhasyimah; Mustafah, Nadia Mohd; Fauzi, Aishah Ahmad; Hashim, Natiara Mohamad

2017-01-01

Extramedullary hematopoiesis (EMH) is the production of blood cell precursors outside the bone marrow that occur in various hematological diseases. In patients with thalassemia intermedia, ineffective erythropoiesis drives compensatory EMH in the liver, pancreas, pleura, spleen, ribs and spine. We describe a patient with thalassemia intermedia who presented with acute neurological symptoms caused by paraspinal EMH, which responded well to combination therapy of steroid, hypertransfusion, laminectomy and excision of pseudotumor and hydroxyurea therapy to boost the formation of fetal haemoglobin. Prompt recognition of EMH based on clinical presentation and typical radiological findings should be made. Early treatment is recommended to prevent irreversible damage to the spinal cord.

Prevotella intermedia stimulates tissue-type plasminogen activator and plasminogen activator inhibitor-2 expression via multiple signaling pathways in human periodontal ligament cells.

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Guan, Su-Min; He, Jian-Jun; Zhang, Ming; Shu, Lei

2011-06-01

Prevotella intermedia is an important periodontal pathogen that induces various inflammatory and immune responses. In this study, we investigated the effects of P. intermedia on the plasminogen system in human periodontal ligament (hPDL) cells and explored the signaling pathways involved. Using semi-quantitative reverse transcription (RT)-PCR and quantitative real-time RT-qPCR, we demonstrated that P. intermedia challenge increased tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor (PAI)-2 expression in a concentration- and time-dependent manner, but exerted no influence on urokinase-type plasminogen activator and PAI-1mRNA expression in hPDL cells. Prevotella intermedia stimulation also enhanced tPA protein secretion as confirmed by enzyme-linked immunosorbent assay. Western blot results revealed that P. intermedia treatment increased phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 kinase (p38). ERK, JNK and protein kinase C inhibitors significantly attenuated the P. intermedia-induced tPA and PAI-2 expression. Furthermore, p38 and phosphatidylinositol 3-kinase inhibitors markedly decreased PAI-2 expression, whereas they showed no or little inhibition on tPA expression. In contrast, inhibition of protein kinase A greatly enhanced the upregulatory effect of P. intermedia on tPA and PAI-2 expression. Our results suggest that P. intermedia may contribute to periodontal tissue destruction by upregulating tPA and PAI-2 expression in hPDL cells via multiple signaling pathways. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

THALASSAEMIA INTERMEDIA : AN UPDATE

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Ali Taher

2009-08-01

Full Text Available Our understanding of the molecular and pathophysiological mechanisms underlying the disease process in patients with thalassaemia intermedia (TI has substantially increased over the past decade. TI encompasses a wide clinical spectrum of beta-thalassaemia phenotypes. Some TI patients are asymptomatic until adult life, whereas others are symptomatic from as young as 2 years of age. A number of clinical complications commonly associated with TI are rarely seen in thalassaemia major, including extramedullary hematopoiesis, leg ulcers, gallstones, thrombosis and pulmonary hypertension. There are a number of options currently available for managing patients with TI, including transfusion therapy, iron chelation therapy, modulation of foetal haemoglobin production and haematopoietic stem cell transplantation. However, at present, there are no clear guidelines for an orchestrated optimal treatment plan.

Reconstruction of chromosome rearrangements between the two most ancestral duckweed species Spirodela polyrhiza and S. intermedia.

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Hoang, Phuong T N; Schubert, Ingo

2017-12-01

The monophyletic duckweeds comprising five genera within the monocot order Alismatales are neotenic, free-floating, aquatic organisms with fast vegetative propagation. Some species are considered for efficient biomass production, for life stock feeding, and for (simultaneous) wastewater phytoremediation. The ancestral genus Spirodela consists of only two species, Spirodela polyrhiza and Spirodela intermedia, both with a similar small genome (~160 Mbp/1C). Reference genome drafts and a physical map of 96 BACs on the 20 chromosome pairs of S. polyrhiza strain 7498 are available and provide useful tools for further evolutionary studies within and between duckweed genera. Here we applied sequential comparative multicolor fluorescence in situ hybridization (mcFISH) to address homeologous chromosomes in S. intermedia (2n = 36), to detect chromosome rearrangements between both species and to elucidate the mechanisms which may have led to the chromosome number alteration after their evolutionary separation. Ten chromosome pairs proved to be conserved between S. polyrhiza and S. intermedia, the remaining ones experienced, depending on the assumed direction of evolution, translocations, inversion, and fissions, respectively. These results represent a first step to unravel karyotype evolution among duckweeds and are anchor points for future genome assembly of S. intermedia.

Characteristics of β-lactamases and their genes (blaA and blaB in Yersinia intermedia and Y. frederiksenii

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Sharma Sachin

2007-04-01

Full Text Available Abstract Background The presence of β-lactamases in Y. enterocolitica has been reported to vary with serovars, biovars and geographical origin of the isolates. An understanding of the β-lactamases in other related species is important for an overall perception of antibiotic resistance in yersiniae. The objective of this work was to study the characteristics of β-lactamases and their genes in strains of Y. intermedia and Y. frederiksenii, isolated from clinical and non-clinical sources in India. Results The enzymes, Bla-A (a constitutive class A penicillinase and Bla-B (an inducible class C cephalosporinase were found to be present in all the clinical and non-clinical strains of Y. intermedia and Y. frederiksenii by double disc diffusion method. The results showed differential expression of Bla-A as indicated by presence/absence of synergy whereas expression of Bla-B was quite consistent. The presence of these enzymes was also reflected in the high minimum inhibitory concentrations, MIC50 (126–1024 mg/L and MIC90 (256–1024 mg/L of β-lactam antibiotics against these species. Restriction fragment length polymorphism (RFLP revealed heterogeneity in both blaA and blaB genes of Y. intermedia and Y. frederiksenii. The blaA gene of Y. intermedia shared significant sequence identity (87–96% with blaA of Y. enterocolitica biovars 1A, 1B and 4. The sequence identity of blaA of Y. frederiksenii with these biovars was 77–79%. The sequence identity of blaB gene of Y. intermedia and Y. frederiksenii was more (85% with that of Y. enterocolitica biovars 1A, 1B and 2 compared to other species viz., Y. bercovieri, Y. aldovae and Y. ruckeri. Isoelectric focusing data further revealed that both Y. intermedia and Y. frederiksenii produced Bla-A (pI 8.7 and "Bla-B like" (pI 5.5–7.1 enzymes. Conclusion Both Y. intermedia and Y. frederiksenii showed presence of blaA and blaB genes and unequivocal expression of the two β-lactamases. Limited heterogeneity

Content-based intermedia synchronization

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Oh, Dong-Young; Sampath-Kumar, Srihari; Rangan, P. Venkat

1995-03-01

Inter-media synchronization methods developed until now have been based on syntactic timestamping of video frames and audio samples. These methods are not fully appropriate for the synchronization of multimedia objects which may have to be accessed individually by their contents, e.g. content-base data retrieval. We propose a content-based multimedia synchronization scheme in which a media stream is viewed as hierarchial composition of smaller objects which are logically structured based on the contents, and the synchronization is achieved by deriving temporal relations among logical units of media object. content-based synchronization offers several advantages such as, elimination of the need for time stamping, freedom from limitations of jitter, synchronization of independently captured media objects in video editing, and compensation for inherent asynchronies in capture times of video and audio.

In vitro effects of N-acetyl cysteine alone and in combination with antibiotics on Prevotella intermedia.

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Moon, Ji-Hoi; Jang, Eun-Young; Shim, Kyu Sang; Lee, Jin-Yong

2015-05-01

N-acetyl cysteine (NAC) is an antioxidant that possesses anti-inflammatory activities in tissues. In the field of dentistry, NAC was demonstrated to prevent the expression of LPS-induced inflammatory mediators in phagocytic cells and gingival fibroblasts during the inflammatory process, but the effect of NAC on oral pathogens has been rarely studied. Here, we examined the effect of NAC against planktonic and biofilm cells of Prevotella intermedia, a major oral pathogen. NAC showed antibacterial activity against the planktonic P. intermedia with MIC value of 3 mg/ml and significantly decreased biofilm formation by the bacterium even at sub MIC. NAC did not affect the antibiotic susceptibility of planktonic P. intermedia, showing indifference (fractional inhibitory concentration index of 0.5-4) results against the bacterium in combination with ampicillin, ciprofloxacin, tetracycline or metronidazole. On the other hand, viability of the pre-established bacterial biofilm exposed to the antibiotics except metronidazole was increased in the presence of NAC. Collectively, NAC may be used for prevention of the biofilm formation by P. intermedia rather than eradication of the pre-established bacterial biofilm. Further studies are required to explore antibacterial and anti-biofilm activity of NAC against mixed population of oral bacteria and its modulatory effect on antibiotics used for oral infectious diseases.

Digital Distribution of Advertising for Publications (DDAP): a graphic arts prototype of electronic intermedia publishing (EIP)

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Dunn, Patrice M.

1998-01-01

The Digital Distribution of Advertising for Publications (DDAP) is a graphic arts industry prototype of Electronic Intermedia Publishing (EIP). EIP is a strategic, multi- industrial concept that seeks to enable the capture and input of volumes of data (i.e., both raster and object oriented data -- as well as the latter's antecedent which is vector data -- color data and black-and-white data) from a multiplicity of devices; then flowing, controlling, manipulating, modifying, storing, retrieving, transmitting, and shipping, that data through an industrial process for output to a multiplicity of output devices (e.g., ink on paper, toner on paper, bits and bytes on CD ROM, Internet, Multimedia, HDTV, etc.). As the technical requirements of the print medium are among the most rigorous in the Intermedia milieu the DDAP prototype addresses some of the most challenging issues faced in Electronic Intermedia Publishing (EIP).

Del Distrito Industrial al Desarrollo Territorial: estrategias de innovación en ciudades intermedias

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Ricardo Méndez

2006-01-01

Full Text Available Los contrastes territoriales se ha incrementado desde el inicio de los procesos de globalización e integración, pero, al mismo tiempo, las ciudades están mostrando diferente capacidad de respuesta ante esas transformaciones. Dentro de la Unión Europea, el policentrismo y el papel de las ciudades intermedias han cobrado creciente importancia en la actual agenda política, especialmente en el caso de las regiones periféricas con problemas estructurales que dificultan su desarrollo. El artículo hace una revisión crítica del paradigma de los distritos industriales y los ambientes innovadores para centrarse en su posible utilidad en la búsqueda de las claves para la construcción de ciudades intermedias innovadoras. Se concede especial importancia a los actores locales, las redes institucionales, la organización de sistemas productivos locales y la gobernabilidad territorial. Por último, se plantean algunas propuestas para apoyar la consolidación de procesos innovativos en ciudades intermedias.

Diploid hybrid origin of Ostryopsis intermedia (Betulaceae) in the Qinghai-Tibet Plateau triggered by Quaternary climate change.

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Liu, Bingbing; Abbott, Richard J; Lu, Zhiqiang; Tian, Bin; Liu, Jianquan

2014-06-01

Despite the well-known effects that Quaternary climate oscillations had on shaping intraspecific diversity, their role in driving homoploid hybrid speciation is less clear. Here, we examine their importance in the putative homoploid hybrid origin and evolution of Ostryopsis intermedia, a diploid species occurring in the Qinghai-Tibet Plateau (QTP), a biodiversity hotspot. We investigated interspecific relationships between this species and its only other congeners, O. davidiana and O. nobilis, based on four sets of nuclear and chloroplast population genetic data and tested alternative speciation hypotheses. All nuclear data distinguished the three species clearly and supported a close relationship between O. intermedia and the disjunctly distributed O. davidiana. Chloroplast DNA sequence variation identified two tentative lineages, which distinguished O. intermedia from O. davidiana; however, both were present in O. nobilis. Admixture analyses of genetic polymorphisms at 20 SSR loci and sequence variation at 11 nuclear loci and approximate Bayesian computation (ABC) tests supported the hypothesis that O. intermedia originated by homoploid hybrid speciation from O. davidiana and O. nobilis. We further estimated that O. davidiana and O. nobilis diverged 6-11 Ma, while O. intermedia originated 0.5-1.2 Ma when O. davidiana is believed to have migrated southward, contacted and hybridized with O. nobilis possibly during the largest Quaternary glaciation that occurred in this region. Our findings highlight the importance of Quaternary climate change in the QTP in causing hybrid speciation in this important biodiversity hotspot. © 2014 John Wiley & Sons Ltd.

Extramedullary hematopoiesis with spinal cord compression in a child with thalassemia intermedia.

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Ileri, Talia; Azik, Fatih; Ertem, Mehmet; Uysal, Zumrut; Gozdasoglu, Sevgi

2009-09-01

Spinal cord compression due to extramedullary hematopoiesis is an extremely rare complication of thalassemia intermedia. No cases with this complication have been reported in the first decade of life, because masses of heterotropic marrow developed in patients as a result of continuous erythropoiesis. We report the 9-year-old patient suffering from thalassemia intermedia and presenting spinal cord compression. We also review the literature about treatment options, because there is no consensus about the optimal treatment of these patients. Our patient was successfully treated with radiation therapy followed by hydroxyurea. With this combination therapy, he had no recurrence during the 4-year follow-up period. Clinical awareness of this phenomenon with the early treatment is essential for optimizing the successful outcome.

Resisting the siren call of individualism in pediatric decision-making and the role of relational interests.

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Salter, Erica K

2014-02-01

The siren call of individualism is compelling. And although we have recognized its dangerous allure in the realm of adult decision-making, it has had profound and yet unnoticed dangerous effects in pediatric decision-making as well. Liberal individualism as instantiated in the best interest standard conceptualizes the child as independent and unencumbered and the goal of child rearing as rational autonomous adulthood, a characterization that is both ontologically false and normatively dangerous. Although a notion of the individuated child might have a place in establishing a threshold of care obligated and enforced by the state, beyond this context we should turn our attention more explicitly to the relational interests of children.

DHA suppresses Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.

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Choi, Eun-Young; Jin, Ji-Young; Choi, Jeom-Il; Choi, In Soon; Kim, Sung-Jo

2014-04-14

Several reports have indicated that dietary intake of DHA is associated with lower prevalence of periodontitis. In the present study, we investigated the effect of DHA on the production of proinflammatory mediators in murine macrophage-like RAW264.7 cells stimulated with lipopolysaccharide (LPS) isolated from Prevotella intermedia, a pathogen implicated in inflammatory periodontal disease, and its mechanisms of action. LPS was isolated from lyophilised P. intermedia ATCC 25,611 cells using the standard hot-phenol-water protocol. Culture supernatants were collected and assayed for NO, IL-1β and IL-6. Real-time PCR analysis was carried out to detect the expression of inducible NO synthase (iNOS), IL-1β, IL-6 and haeme oxygenase-1 (HO-1) mRNA. Immunoblot analysis was carried out to quantify the expression of iNOS and HO-1 protein and concentrations of signalling proteins. DNA-binding activities of NF-κB subunits were determined using an ELISA-based assay kit. DHA significantly attenuated the production of NO, IL-1β and IL-6 at both gene transcription and translation levels in P. intermedia LPS-activated RAW264.7 cells. DHA induced the expression of HO-1 in cells treated with P. intermedia LPS. Selective inhibition of HO-1 activity by tin protoporphyrin IX significantly mitigated the inhibitory effects of DHA on LPS-induced NO production. DHA significantly attenuated the phosphorylation of c-Jun N-terminal kinase induced by LPS. In addition, DHA suppressed the transcriptional activity of NF-κB by regulating the nuclear translocation and DNA-binding activity of NF-κB p50 subunit and inhibited the phosphorylation of signal transducer and activator of transcription 1. Further in vivo studies are needed to better evaluate the potential of DHA in humans as a therapeutic agent to treat periodontal disease.

A Preliminary Study of the Effects of pH upon Fluorescence in Suspensions of Prevotella intermedia.

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Hope, Christopher K; Billingsley, Karen; de Josselin de Jong, Elbert; Higham, Susan M

2016-01-01

The quantification of fluorescence in dental plaque is currently being developed as a diagnostic tool to help inform and improve oral health. The oral anaerobe Prevotella intermedia exhibits red fluorescence due to the accumulation of porphyrins. pH affects the fluorescence of abiotic preparations of porphyrins caused by changes in speciation between monomers, higher aggregates and dimers, but this phenomenon has not been demonstrated in bacteria. Fluorescence spectra were obtained from suspensions of P. intermedia that were adjusted to pHs commensurate with the range found within dental plaque. Two fluorescent motifs were identified; 410 nm excitation / 634 nm emission (peak A) and 398 nm excitation / 622 nm emission (peak B). A transition in the fluorescence spectra was observed from peak A to peak B with increasing pH which was also evident as culture age increased from 24 hours to 96 hours. In addition to these 'blue-shifts', the intensity of peak A increased with pH whilst decreasing with culture age from 24 to 96 hours. A bacterium's relationship with the local physiochemical environment at the time of image capture may therefore affect the quantification of dental plaque fluorescence.

Evaluation of the efficacy of vacuum cleaners for the integrated control of brown spider Loxosceles intermedia

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E. N. Ramires

2007-01-01

Full Text Available Some venomous spiders of the genus Loxosceles can reach high population densities inside and around houses. In Brazil, most spider accidents are related to Loxosceles intermedia. Control of loxoscelism should utilize integrated pest management tools, such as vacuum cleaners, to eliminate egg sacs, webs and spiders. The present study tested the efficacy of one type of vacuum cleaner (for professional and domestic use in the control of L. intermedia populations. Cockroaches (Pycnoscelus surinamensis were used in some tests for comparison. Vacuuming using standard accessories or a paper tube resulted in the death of all female (n=60, male (n=60, young (n=60 and just-hatched (n=60 L. intermedia, and all egg sacs (n=5 were destroyed. The removal of the plastic plate present at the bottom of the vacuuming tube inside the machine allowed some spiders to survive the vacuuming process. When kept inside a vacuum bag full of dust and debris, adult females (n=10 survived for 10 days; however, significant mortality was observed among male (n=10 and young individuals (n=10. Addition of cornstarch to the vacuum bag did not affect the spiders (n=20. Vacuum cleaners, such as the one used in the present investigation, are promising tools for integrated management of L. intermedia and other spiders in domestic environments.

Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood.

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Weinkopff, Tiffany; de Oliveira, Camila I; de Carvalho, Augusto M; Hauyon-La Torre, Yazmin; Muniz, Aline C; Miranda, Jose Carlos; Barral, Aldina; Tacchini-Cottier, Fabienne

2014-01-01

During a blood meal, Lutzomyia intermedia sand flies transmit Leishmania braziliensis, a parasite causing tegumentary leishmaniasis. In experimental leishmaniasis, pre-exposure to saliva of most blood-feeding sand flies results in parasite establishment in absence of any skin damages in mice challenged with dermotropic Leishmania species together with saliva. In contrast, pre-immunization with Lu. intermedia salivary gland sonicate (SGS) results in enhanced skin inflammatory exacerbation upon co-inoculation of Lu. intermedia SGS and L. braziliensis. These data highlight potential unique features of both L. braziliensis and Lu. intermedia. In this study, we investigated the genes modulated by Lu. intermedia SGS immunization to understand their potential impact on the subsequent cutaneous immune response following inoculation of both SGS and L. braziliensis. The cellular recruitment and global gene expression profile was analyzed in mice repeatedly inoculated or not with Lu. intermedia. Microarray gene analysis revealed the upregulation of a distinct set of IFN-inducible genes, an immune signature not seen to the same extent in control animals. Of note this INF-inducible gene set was not induced in SGS pre-immunized mice subsequently co-inoculated with SGS and L. braziliensis. These data suggest the parasite prevented the upregulation of this Lu. intermedia saliva-related immune signature. The presence of these IFN-inducible genes was further analyzed in peripheral blood mononuclear cells (PBMCs) sampled from uninfected human individuals living in a L. braziliensis-endemic region of Brazil thus regularly exposed to Lu. intermedia bites. PBMCs were cultured in presence or absence of Lu. intermedia SGS. Using qRT-PCR we established that the IFN-inducible genes induced in the skin of SGS pre-immunized mice, were also upregulated by SGS in PBMCs from human individuals regularly exposed to Lu. intermedia bites, but not in PBMCs of control subjects. These data demonstrate

Prevotella intermedia infection causing acute and complicated aortitis-A case report.

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Boersma, C; Kampschreur, L M; Buter, H; Doorenbos, B M; Klinkert, P; Koning, G G

2017-01-01

Aortitis is a general term that refers to all conditions involving an inflammation of the aortic wall. This case report describes the surgical approach of a patient with infectious and symptomatic aortitis caused by the rare vector Prevotella intermedia. A 44-year old male patient was admitted with fever and general discomfort after a period of sore throat in a non-teaching hospital. After two weeks he developed acute abdominal and back pain accompanied by sweating and elevated infection parameters. Computed tomography angiography revealed atherosclerotic changes of the infrarenal aorta with a locally contained rupture of the aorta alongside peri-aortal signs of inflammation (and aortitis aspects). An urgent aortic reconstruction was performed according to Nevelsteen. The blood cultures turned out positive for Prevotella intermedia. Postoperatively the patient received antibiotics for six weeks. The patient recovered uneventful from this infection and surgical procedure. A complicated and acute aortitis is a rare but potentially life-threatening disease. The aetiology can be ordered into two main groups; inflammatory and infectious. Diagnosis is based upon symptoms, biochemical values, microbiological results and imaging modalities. Treatment depends on aetiology and should be discussed in an experienced multidisciplinary setting. Infectious aortitis should be treated with antibiotics for at least six weeks with close monitoring of the patient's clinic and biochemical values, even after surgery. Prevotella intermedia is a rare causative agent for aortitis. Acute aortitis is a challenging clinical entity which should be managed in an equipped medical center by an experienced multidisciplinary team. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

Morphology, morphogenesis, and phylogeny of an Anteholosticha intermedia (Ciliophora, Urostylida) population from the United States.

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Chen, Lingyun; Wu, Weining; El-Serehy, Hamed A; Hu, Xiaozhong; Clamp, John C

2018-04-30

A distinct population of Anteholosticha intermedia was isolated from soil in the Great Smoky Mountains of North Carolina, USA, and its morphology, morphogenesis and molecular phylogeny investigated by microscopic observations of live and protargol-prepared specimens and analyses of the sequence of small subunit (SSU) rDNA. Our population closely resembles the populations from Austria and Korea. Members of the genus Anteholosticha have been regarded as ontogenetically diverse, which is confirmed by the present work. The most noteworthy ontogenetic feature of the American population of A. intermedia is that the oral primordium in the proter appears apokinetally at the posterior end of the undulating membranes anlage at the beginning of division and then dedifferentiates midway through morphogenesis. Molecular phylogenetic analyses demonstrate, with high support, that the American population of A. intermedia is clearly distinct from congeners and branches as part of a sister lineage to the Bakuella-Urostyla clade that belongs to the major clade comprising the order Urostylida. Copyright © 2018 Elsevier GmbH. All rights reserved.

Population-Genomic Insights into Variation in Prevotella intermedia and Prevotella nigrescens Isolates and Its Association with Periodontal Disease

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Yifei Zhang

2017-09-01

Full Text Available High-throughput sequencing has helped to reveal the close relationship between Prevotella and periodontal disease, but the roles of subspecies diversity and genomic variation within this genus in periodontal diseases still need to be investigated. We performed a comparative genome analysis of 48 Prevotella intermedia and Prevotella nigrescens isolates that from the same cohort of subjects to identify the main drivers of their pathogenicity and adaptation to different environments. The comparisons were done between two species and between disease and health based on pooled sequences. The results showed that both P. intermedia and P. nigrescens have highly dynamic genomes and can take up various exogenous factors through horizontal gene transfer. The major differences between disease-derived and health-derived samples of P. intermedia and P. nigrescens were factors related to genome modification and recombination, indicating that the Prevotella isolates from disease sites may be more capable of genomic reconstruction. We also identified genetic elements specific to each sample, and found that disease groups had more unique virulence factors related to capsule and lipopolysaccharide synthesis, secretion systems, proteinases, and toxins, suggesting that strains from disease sites may have more specific virulence, particularly for P. intermedia. The differentially represented pathways between samples from disease and health were related to energy metabolism, carbohydrate and lipid metabolism, and amino acid metabolism, consistent with data from the whole subgingival microbiome in periodontal disease and health. Disease-derived samples had gained or lost several metabolic genes compared to healthy-derived samples, which could be linked with the difference in virulence performance between diseased and healthy sample groups. Our findings suggest that P. intermedia and P. nigrescens may serve as “crucial substances” in subgingival plaque, which may

Population-Genomic Insights into Variation in Prevotella intermedia and Prevotella nigrescens Isolates and Its Association with Periodontal Disease.

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Zhang, Yifei; Zhen, Min; Zhan, Yalin; Song, Yeqing; Zhang, Qian; Wang, Jinfeng

2017-01-01

High-throughput sequencing has helped to reveal the close relationship between Prevotella and periodontal disease, but the roles of subspecies diversity and genomic variation within this genus in periodontal diseases still need to be investigated. We performed a comparative genome analysis of 48 Prevotella intermedia and Prevotella nigrescens isolates that from the same cohort of subjects to identify the main drivers of their pathogenicity and adaptation to different environments. The comparisons were done between two species and between disease and health based on pooled sequences. The results showed that both P. intermedia and P. nigrescens have highly dynamic genomes and can take up various exogenous factors through horizontal gene transfer. The major differences between disease-derived and health-derived samples of P. intermedia and P. nigrescens were factors related to genome modification and recombination, indicating that the Prevotella isolates from disease sites may be more capable of genomic reconstruction. We also identified genetic elements specific to each sample, and found that disease groups had more unique virulence factors related to capsule and lipopolysaccharide synthesis, secretion systems, proteinases, and toxins, suggesting that strains from disease sites may have more specific virulence, particularly for P. intermedia . The differentially represented pathways between samples from disease and health were related to energy metabolism, carbohydrate and lipid metabolism, and amino acid metabolism, consistent with data from the whole subgingival microbiome in periodontal disease and health. Disease-derived samples had gained or lost several metabolic genes compared to healthy-derived samples, which could be linked with the difference in virulence performance between diseased and healthy sample groups. Our findings suggest that P. intermedia and P. nigrescens may serve as "crucial substances" in subgingival plaque, which may reflect changes in

The contingency of intermedia agenda setting: a longitudinal study in Belgium

NARCIS (Netherlands)

Vliegenthart, R.; Walgrave, S.

2008-01-01

This large-scale study investigates how intermedia agenda-setting effects are moderated by five factors: (1) lag length; (2) medium type; (3) language/institutional barriers; (4) issue type; and (5) election or non-election context. Longitudinal analyses of daily attention to twenty-five issues in

Amelioration of atopic-like skin conditions in NC/Tnd mice by topical application with distilled Alpinia intermedia Gagnep extracts.

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Amagai, Yosuke; Katsuta, Chihiro; Nomura, Yoshihiro; Oida, Kumiko; Matsuda, Kenshiro; Jang, Hyosun; Ahn, Ginnae; Hamasaki, Tetsuyoshi; Matsuda, Hiroshi; Tanaka, Akane

2017-11-01

Alpinia intermedia, a perennial plant that belongs to the Zingiberaceae family, has been used in folk medicine for a long time in the southern region of Japan. Because skin care is an effective approach that enables patients to manage their atopic dermatitis (AD), various herbal ingredients with few adverse effects have been evaluated for use in AD patients in recent years. In this study, we examined whether distilled extracts obtained from A. intermedia were beneficial for AD-like skin conditions in NC/Tnd mice. Topical application with the A. intermedia extracts significantly reduced the severity of AD, transepidermal water loss and scratching behavior in the mice. Supplementation of the extracts to cell cultures suppressed the expression of Tslp mRNA in PAM212 keratinocytes, degranulation in bone marrow-derived cultured mast cells (BMCMC), and neurite outgrowth in PC12 cells and dorsal root ganglia. In addition, the component analysis revealed that β-pinene was a major constituent of the A. intermedia extracts. The inhibitory effects of β-pinene both in vivo and in vitro were also demonstrated. These results indicate that topical application with the A. intermedia extract to the skin of NC/Tnd mice improved the condition of the skin by suppressing multiple inflammatory responses. The extracts may become novel skin-care remedies for AD patients. © 2017 The Authors. The Journal of Dermatology published by John Wiley & Sons Australia, Ltd on behalf of Japanese Dermatological Association.

Pathogenicity of facultative and obligate anaerobic bacteria in monoculture and combined with either Prevotella intermedia or Prevotella nigrescens.

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Siqueira, J F; Magalhães, F A; Lima, K C; de Uzeda, M

1998-12-01

The pathogenicity of obligate and facultative anaerobic bacteria commonly found in endodontic infections was tested using a mouse model. The capacity of inducing abscesses was evaluated seven days after subcutaneous injection of the bacteria in pure culture and in combinations with either Prevotella intermedia or Prevotella nigrescens. Nine of the fifteen bacterial strains tested were pathogenic in pure culture. No statistically significant differences were detected between these strains in pure culture and in mixtures with either P. intermedia or P. nigrescens. Synergism between the bacterial strains was only apparent when associating Porphyromonas endodontalis with P. intermedia or P. nigrescens. Histopathological examination of tissue sections from induced abscesses revealed an acute inflammatory reaction, dominated by polymorphonuclear leukocytes. Sections from the control group using sterile medium showed no evidence of inflammatory reaction.

Acquisition of complement inhibitor serine protease factor I and its cofactors C4b-binding protein and factor H by Prevotella intermedia.

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Malm, Sven; Jusko, Monika; Eick, Sigrun; Potempa, Jan; Riesbeck, Kristian; Blom, Anna M

2012-01-01

Infection with the Gram-negative pathogen Prevotella intermedia gives rise to periodontitis and a growing number of studies implies an association of P. intermedia with rheumatoid arthritis. The serine protease Factor I (FI) is the central inhibitor of complement degrading complement components C3b and C4b in the presence of cofactors such as C4b-binding protein (C4BP) and Factor H (FH). Yet, the significance of complement inhibitor acquisition in P. intermedia infection and FI binding by Gram-negative pathogens has not been addressed. Here we show that P. intermedia isolates bound purified FI as well as FI directly from heat-inactivated human serum. FI bound to bacteria retained its serine protease activity as shown in degradation experiments with (125)I-labeled C4b. Since FI requires cofactors for its activity we also investigated the binding of purified cofactors C4BP and FH and found acquisition of both proteins, which retained their activity in FI mediated degradation of C3b and C4b. We propose that FI binding by P. intermedia represents a new mechanism contributing to complement evasion by a Gram-negative bacterial pathogen associated with chronic diseases.

Rhamnus intermedia Steud. et Hochst. - a New Source of Bioactive Phytochemicals

Czech Academy of Sciences Publication Activity Database

Samec, D.; Kremer, D.; Grúz, Jiří; Grubešić, R. J.; Piljac-Žegarac, J.

2012-01-01

Roč. 85, č. 2 (2012), s. 125-129 ISSN 0011-1643 R&D Projects: GA AV ČR KAN200380801 Institutional research plan: CEZ:AV0Z50380511 Keywords : Rhamnus intermedia * antioxidant activity * total phenols Subject RIV: EC - Immunology Impact factor: 0.614, year: 2012

Different water use strategies of juvenile and adult Caragana intermedia plantations in the Gonghe Basin, Tibet Plateau.

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Zhiqing Jia

Full Text Available In a semi-arid ecosystem, water is one of the most important factors that affect vegetation dynamics, such as shrub plantation. A water use strategy, including the main water source that a plant species utilizes and water use efficiency (WUE, plays an important role in plant survival and growth. The water use strategy of a shrub is one of the key factors in the evaluation of stability and sustainability of a plantation.Caragana intermedia is a dominant shrub of sand-binding plantations on sand dunes in the Gonghe Basin in northeastern Tibet Plateau. Understanding the water use strategy of a shrub plantation can be used to evaluate its sustainability and long-term stability. We hypothesized that C. intermedia uses mainly deep soil water and its WUE increases with plantation age. Stable isotopes of hydrogen and oxygen were used to determine the main water source and leaf carbon isotope discrimination was used to estimate long-term WUE. The root system was investigated to determine the depth of the main distribution. The results showed that a 5-year-old C. intermedia plantation used soil water mainly at a depth of 0-30 cm, which was coincident with the distribution of its fine roots. However, 9- or 25-year-old C. intermedia plantations used mainly 0-50 cm soil depth water and the fine root system was distributed primarily at soil depths of 0-50 cm and 0-60 cm, respectively. These sources of soil water are recharged directly by rainfall. Moreover, the long-term WUE of adult plantations was greater than that of juvenile plantations.The C. intermedia plantation can change its water use strategy over time as an adaptation to a semi-arid environment, including increasing the depth of soil water used for root growth, and increasing long-term WUE.

Different Water Use Strategies of Juvenile and Adult Caragana intermedia Plantations in the Gonghe Basin, Tibet Plateau

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Jia, Zhiqing; Zhu, Yajuan; Liu, Liying

2012-01-01

Background In a semi-arid ecosystem, water is one of the most important factors that affect vegetation dynamics, such as shrub plantation. A water use strategy, including the main water source that a plant species utilizes and water use efficiency (WUE), plays an important role in plant survival and growth. The water use strategy of a shrub is one of the key factors in the evaluation of stability and sustainability of a plantation. Methodology/Principal Findings Caragana intermedia is a dominant shrub of sand-binding plantations on sand dunes in the Gonghe Basin in northeastern Tibet Plateau. Understanding the water use strategy of a shrub plantation can be used to evaluate its sustainability and long-term stability. We hypothesized that C. intermedia uses mainly deep soil water and its WUE increases with plantation age. Stable isotopes of hydrogen and oxygen were used to determine the main water source and leaf carbon isotope discrimination was used to estimate long-term WUE. The root system was investigated to determine the depth of the main distribution. The results showed that a 5-year-old C. intermedia plantation used soil water mainly at a depth of 0–30 cm, which was coincident with the distribution of its fine roots. However, 9- or 25-year-old C. intermedia plantations used mainly 0–50 cm soil depth water and the fine root system was distributed primarily at soil depths of 0–50 cm and 0–60 cm, respectively. These sources of soil water are recharged directly by rainfall. Moreover, the long-term WUE of adult plantations was greater than that of juvenile plantations. Conclusions The C. intermedia plantation can change its water use strategy over time as an adaptation to a semi-arid environment, including increasing the depth of soil water used for root growth, and increasing long-term WUE. PMID:23029303

Morphology, ecology, and behaviour of Hylarana intermedia, a Western Ghats frog

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Ambika Kamath

2016-06-01

Full Text Available Despite being common in the Western Ghats-Sri Lanka biodiversity hotspot, Golden-backed frogs (Hylarana, Ranidae remain poorly studied. In this paper, we present some preliminary data on the morphology, behaviour, and habitat use of Hylarana intermedia, a member of the Hylarana aurantiaca species group. We find evidence for female biased size dimorphism, as well as potential shape differences between the sexes in this species. Additionally, we investigate the relationships between traits that may contribute to male conspicuousness (call rates, dorsal coloration, and body size in two breeding habitats, a paddy field and a trench. Our results suggest both size-dependent and environment-dependent variation in call rate and colour in this species. Specifically, we find evidence against the adoption of sneaker mating strategies by small males in H. intermedia, and instead find variation both within and between populations in traits contributing to male conspicuousness. We conclude by proposing future directions for research on this common frog species.

Intraspecies Variability Affects Heterotypic Biofilms of Porphyromonas gingivalis and Prevotella intermedia: Evidences of Strain-Dependence Biofilm Modulation by Physical Contact and by Released Soluble Factors.

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Graziela Murta Barbosa

Full Text Available It is well known that strain and virulence diversity exist within the population structure of Porphyromonas gingivalis. In the present study we investigate intra- and inter-species variability in biofilm formation of Porphyromonas gingivalis and partners Prevotella intermedia and Prevotella nigrescens. All strains tested showed similar hydrophobicity, except for P. gingivalis W83 which has roughly half of the hydrophobicity of P. gingivalis ATCC33277. An intraspecies variability in coaggregation of P. gingivalis with P. intermedia was also found. The association P. gingivalis W83/P. intermedia 17 produced the thickest biofilm and strain 17 was prevalent. In a two-compartment system P. gingivalis W83 stimulates an increase in biomass of strain 17 and the latter did not stimulate the growth of P. gingivalis W83. In addition, P. gingivalis W83 also stimulates the growth of P. intermedia ATCC25611 although strain W83 was prevalent in the association with P. intermedia ATCC25611. P. gingivalis ATCC33277 was prevalent in both associations with P. intermedia and both strains of P. intermedia stimulate the growth of P. gingivalis ATCC33277. FISH images also showed variability in biofilm structure. Thus, the outcome of the association P. gingivalis/P. intermedia seems to be strain-dependent, and both soluble factors and physical contact are relevant. The association P. gingivalis-P. nigrescens ATCC33563 produced larger biomass than each monotypic biofilm, and P. gingivalis was favored in consortia, while no differences were found in the two-compartment system. Therefore, in consortia P. gingivalis-P. nigrescens physical contact seems to favor P. gingivalis growth. The intraspecies variability found in our study suggests strain-dependence in ability of microorganisms to recognize molecules in other bacteria which may further elucidate the dysbiosis event during periodontitis development giving additional explanation for periodontal bacteria, such as P

Intraspecies Variability Affects Heterotypic Biofilms of Porphyromonas gingivalis and Prevotella intermedia: Evidences of Strain-Dependence Biofilm Modulation by Physical Contact and by Released Soluble Factors.

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Barbosa, Graziela Murta; Colombo, Andrea Vieira; Rodrigues, Paulo Henrique; Simionato, Maria Regina Lorenzetti

2015-01-01

It is well known that strain and virulence diversity exist within the population structure of Porphyromonas gingivalis. In the present study we investigate intra- and inter-species variability in biofilm formation of Porphyromonas gingivalis and partners Prevotella intermedia and Prevotella nigrescens. All strains tested showed similar hydrophobicity, except for P. gingivalis W83 which has roughly half of the hydrophobicity of P. gingivalis ATCC33277. An intraspecies variability in coaggregation of P. gingivalis with P. intermedia was also found. The association P. gingivalis W83/P. intermedia 17 produced the thickest biofilm and strain 17 was prevalent. In a two-compartment system P. gingivalis W83 stimulates an increase in biomass of strain 17 and the latter did not stimulate the growth of P. gingivalis W83. In addition, P. gingivalis W83 also stimulates the growth of P. intermedia ATCC25611 although strain W83 was prevalent in the association with P. intermedia ATCC25611. P. gingivalis ATCC33277 was prevalent in both associations with P. intermedia and both strains of P. intermedia stimulate the growth of P. gingivalis ATCC33277. FISH images also showed variability in biofilm structure. Thus, the outcome of the association P. gingivalis/P. intermedia seems to be strain-dependent, and both soluble factors and physical contact are relevant. The association P. gingivalis-P. nigrescens ATCC33563 produced larger biomass than each monotypic biofilm, and P. gingivalis was favored in consortia, while no differences were found in the two-compartment system. Therefore, in consortia P. gingivalis-P. nigrescens physical contact seems to favor P. gingivalis growth. The intraspecies variability found in our study suggests strain-dependence in ability of microorganisms to recognize molecules in other bacteria which may further elucidate the dysbiosis event during periodontitis development giving additional explanation for periodontal bacteria, such as P. gingivalis and P

Estabelecimento de colonia, em laboratório, de Lutzomyia intermedia Lutz & Neiva, 1912 (Diptera, Psychodidae, Phlebotominae Establishment of colony, in laboratory, of Lutzomyia intermedia Lutz & Neiva, 1912 (Diptera, Psychodidae, Phlebotominae

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Elizabeth F. Rangel

1985-06-01

Full Text Available Para a utilização em infecções experimentais e xenodiagnósticos de infecções naturais por leishmânias dermotrópicas do Rio de Janeiro, estabelecemos, em laboratório, uma colônia de Lutzomyia intermedia apresentando aqui a metodologia seguida, juntamente com dados relativos ao rendimento e duração de cada fase evolutiva nas quatro primeiras gerações.A laboratory colony of the sandfly Lutzomyia intermedia was established (at present in its seventh generation in order to provide specimens for experimental infection and for experimental xenodiagnosis of natural infections due to cutaneous leishmanias in Rio de Janeiro. Date are presented on the methodology and on the yield and duration of each stage of development, during the first four generations.

Comparison of right and left side heart functions in patients with thalassemia major, patients with thalassemia intermedia, and control group.

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Noori, Noormohammad; Mohamadi, Mehdi; Keshavarz, Kambiz; Alavi, Seyed Mostafa; Mahjoubifard, Maziar; Mirmesdagh, Yalda

2013-01-01

Heart disease is the main cause of mortality and morbidity in patients with beta thalassemia, rendering its early diagnosis vital. We studied and compared echocardiographic findings in patients with beta thalassemia major, patients with beta thalassemia intermedia, and a control group. Eighty asymptomatic patients with thalassemia major and 22 asymptomatic cases with thalassemia intermedia (8-25 years old) were selected from those referred to Ali Asghar Hospital (Zahedan-Iran) between June 2008 and June 2009. Additionally, 80 healthy individuals within the same age and sex groups were used as controls. All the individuals underwent echocardiography, the data of which were analyzed with the Student t-test. The mean value of the pre-ejection period/ejection time ratio of the left ventricle during systole, the diameter of the posterior wall of the left ventricle during diastole, the left and right isovolumic relaxation times, and the right myocardial performance index in the patients with beta thalassemia major and intermedia increased significantly compared to those of the controls, but the other parameters were similar between the two patient groups. The mean values of the left and right pre-ejection periods, left ventricular end systolic dimension, and left isovolumic contraction time in the patients with thalassemia intermedia increased significantly compared to those of the controls. In the left side, myocardial performance index, left ventricular mass index, isovolumic contraction time, and deceleration time exhibited significant changes between the patients with thalassemia major and those with thalassemia intermedia, whereas all the echocardiographic parameters of the right side were similar between these two groups. The results showed that the systolic and diastolic functions of the right and left sides of the heart would be impaired in patients with thalassemia major and thalassemia intermedia. Consequently, serial echocardiography is suggested in

Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.

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Natalia M Tavares

Full Text Available BACKGROUND: Leishmania transmission occurs in the presence of insect saliva. Immunity to Phlebotomus papatasi or Lutzomyia longipalpis saliva or salivary components confers protection against an infection by Leishmania in the presence of the homologous saliva. However, immunization with Lutzomyia intermedia saliva did not protect mice against Leishmania braziliensis plus Lu. intermedia saliva. In the present study, we have studied whether the immunization with Lu. longipalpis saliva or a DNA plasmid coding for LJM19 salivary protein would be protective against L. braziliensis infection in the presence of Lu. intermedia saliva, the natural vector for L. braziliensis. METHODOLOGY/PRINCIPAL FINDINGS: Immunization with Lu. longipalpis saliva or with LJM19 DNA plasmid induced a Delayed-Type Hypersensitivity (DTH response against Lu. longipalpis as well as against a Lu. intermedia saliva challenge. Immunized and unimmunized control hamsters were then intradermally infected in the ears with L. braziliensis in the presence of Lu. longipalpis or Lu. intermedia saliva. Animals immunized with Lu. longipalpis saliva exhibited smaller lesion sizes as well as reduced disease burdens both at lesion site and in the draining lymph nodes. These alterations were associated with a significant decrease in the expression levels of IL-10 and TGF-β. Animals immunized with LJM19 DNA plasmid presented similar findings in protection and immune response and additionally increased IFN-γ expression. CONCLUSIONS/SIGNIFICANCE: Immunization with Lu. longipalpis saliva or with a DNA plasmid coding LJM19 salivary protein induced protection in hamsters challenged with L. braziliensis plus Lu. intermedia saliva. These findings point out an important role of immune response against saliva components, suggesting the possibility to develop a vaccine using a single component of Lu. longipalpis saliva to generate protection against different species of Leishmania, even those

Effect of pollen irradiation on hybridization of wheat and Eltrigia intermedia and their embryo development

International Nuclear Information System (INIS)

Li Guiying; Wang Linqing; Shi Jinguo

2005-01-01

Spikes of Eltrigia intermedia were radiated with 5-100 Gy γ-ray during anthesis, and then their pollens were collected to pollinate to the common wheat 'J-11' and 'Chinese Spring'. The effects of pollen irradiation on the seed setting, embryo development, embryo culture and plantlet rate were studied. The results showed that low dose (5-9 Gy) of irradiation enhanced the seed setting for Chinese spring x E. intermedia, but no such effect for J-11xE. intermedia. Irradiation with all doses damaged embryo development, percentage of seeds with embryos; rate of immature hybrid embryos developing into plantlets decreases with the increased doses. Percentage of seeds with abnormal embryos increased significantly with the doses. 12.9%-14.5% of embryos could develop into plants in 30 Gy treatment, which seldom occur to wheat. Embryos in 50 Gy-100 Gy treatment were affected so serious that even none of them could develop into plants in vitro culture. It may be an effective approach to obtaining more mutants and enhancing induced mutation breeding by using combination of pollen irradiation, immature embryo rescue and anther culture of the resultant progenies. (authors)

Beta-thalassemia intermedia associated with moyamoya syndrome.

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Göksel, Basak Karakurum; Ozdogu, Hakan; Yildirim, Tulin; Oğuzkurt, Levent; Asma, Suheyl

2010-07-01

Moyamoya syndrome (MMS) is a progressive disorder. We report a 19-year-old boy with beta-thalassemia who presented with a left hemiparesis. Brain MRI showed old middle cerebral artery and left frontal subcortical white matter infarcts. Brain magnetic resonance angiography and digital subtraction angiography revealed occlusion of the bilateral internal carotid arteries with a rich network of basal collateral vessels. To our knowledge this is the third report of beta-thalassemia intermedia and MMS, and the first report of a patient in Turkey. It emphasizes the potential for cerebral infarct due to anemia, protein S and thrombocytosis.

Effect of caffeic acid phenethyl ester on Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.

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Choi, E-Y; Choe, S-H; Hyeon, J-Y; Choi, J-I; Choi, I S; Kim, S-J

2015-12-01

Caffeic acid phenethyl ester (CAPE) has numerous potentially beneficial properties, including antioxidant, immunomodulatory and anti-inflammatory activities. However, the effect of CAPE on periodontal disease has not been studied before. This study was designed to investigate the efficacy of CAPE in ameliorating the production of proinflammatory mediators in macrophages activated by lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in periodontal disease. LPS from P. intermedia ATCC 25611 was isolated by using the standard hot phenol-water method. Culture supernatants were assayed for nitric oxide (NO), interleukin (IL)-1β and IL-6. We used real-time polymerase chain reaction to quantify inducible NO synthase, IL-1β, IL-6, heme oxygenase (HO)-1 and suppressors of cytokine signaling (SOCS) 1 mRNA expression. HO-1 protein expression and levels of signaling proteins were assessed by immunoblot analysis. DNA-binding activities of NF-κB subunits were analyzed by using the enzyme-linked immunosorbent assay-based kits. CAPE exerted significant inhibitory effects on P. intermedia LPS-induced production of NO, IL-1β and IL-6 as well as their mRNA expression in RAW264.7 cells. CAPE-induced HO-1 expression in cells activated with P. intermedia LPS, and selective inhibition of HO-1 activity by tin protoporphyrin IX attenuated the inhibitory effect of CAPE on LPS-induced NO production. CAPE did not interfere with IκB-α degradation induced by P. intermedia LPS. Instead, CAPE decreased nuclear translocation of NF-κB p65 and p50 subunits induced with LPS, and lessened LPS-induced p50 binding activity. Further, CAPE showed strong inhibitory effects on LPS-induced signal transducer and activator of transcription 1 and 3 phosphorylation. Besides, CAPE significantly elevated SOCS1 mRNA expression in P. intermedia LPS-stimulated cells. Modulation of host response by CAPE may represent an attractive strategy towards the treatment of periodontal disease

Curcumin suppresses the production of interleukin-6 in Prevotella intermedia lipopolysaccharide-activated RAW 264.7 cells

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2011-01-01

Purpose Curcumin is known to exert numerous biological effects including anti-inflammatory activity. In this study, we investigated the effects of curcumin on the production of interleukin-6 (IL-6) by murine macrophage-like RAW 264.7 cells stimulated with lipopolysaccharide (LPS) from Prevotella intermedia, a major cause of inflammatory periodontal disease, and sought to determine the underlying mechanisms of action. Methods LPS was prepared from lyophilized P. intermedia ATCC 25611 cells by the standard hot phenol-water method. Culture supernatants were collected and assayed for IL-6. We used real-time polymerase chain reaction to detect IL-6 mRNA expression. IκB-α degradation, nuclear translocation of NF-κB subunits, and STAT1 phosphorylation were characterized via immunoblotting. DNA-binding of NF-κB was also analyzed. Results Curcumin strongly suppressed the production of IL-6 at both gene transcription and translation levels in P. intermedia LPS-activated RAW 264.7 cells. Curcumin did not inhibit the degradation of IκB-α induced by P. intermedia LPS. Curcumin blocked NF-κB signaling through the inhibition of nuclear translocation of NF-κB p50 subunit. Curcumin also attenuated DNA binding activity of p50 and p65 subunits and suppressed STAT1 phosphorylation. Conclusions Although further study is required to explore the detailed mechanism of action, curcumin may contribute to blockade of the host-destructive processes mediated by IL-6 and appears to have potential therapeutic values in the treatment of inflammatory periodontal disease. PMID:21811692

Eclectic feeding behavior of Lutzomyia (Nyssomyia intermedia (Diptera, Psychodidae, Phlebotominae in the transmission area of American cutaneous leishmaniasis, state of Paraná, Brazil

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Mauricio Baum

2013-10-01

Full Text Available Introduction The blood meal source of sandflies provides valuable information about the vector/host interaction and allows for an understanding of American cutaneous leishmaniasis (ACL transmission mechanisms. The aim of this study was to identify the blood meal sources of Lutzomyia (Nyssomyia intermedia in an endemic area of leishmaniasis in Brazil's State of Paraná using a precipitin test. Methods Sandflies were collected in the rural locality of Epitácio Pessoa within the City of Adrianópolis, State of Paraná, in southern Brazil. A total of 864 female sandflies were captured, and 862 (99.8% were identified as L. intermedia species. However, two unidentified specimens were considered to be part of the genus Lutzomyia. Results Among the females examined, 396 specimens presented reactions to a certain type of tested antiserum, and most (67.9% reacted to the simple type. These sandflies fed mainly on the blood of birds, opossums, and rodents, but specimens that fed on the blood of humans, dogs, horses, cattle, and cats were also found. Among the cross-reactions found (32.1%, bird/rodent, bird/opossum, bird/dog, bird/human, and horse/dog cross-reactions were the most common. Conclusions These results demonstrate a tendency in the eclectic feeding behavior of L. intermedia and support its potential role as a vector for ACL in the study area.

Eclectic feeding behavior of Lutzomyia (Nyssomyia) intermedia (Diptera, Psychodidae, Phlebotominae) in the transmission area of American cutaneous leishmaniasis, state of Paraná, Brazil.

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Baum, Mauricio; Ribeiro, Magda Clara Vieira da Costa; Lorosa, Elias Seixas; Damasio, Guilherme Augustto Costa; Castro, Edilene Alcântara de

2013-01-01

The blood meal source of sandflies provides valuable information about the vector/host interaction and allows for an understanding of American cutaneous leishmaniasis (ACL) transmission mechanisms. The aim of this study was to identify the blood meal sources of Lutzomyia (Nyssomyia) intermedia in an endemic area of leishmaniasis in Brazil's State of Paraná using a precipitin test. Sandflies were collected in the rural locality of Epitácio Pessoa within the City of Adrianópolis, State of Paraná, in southern Brazil. A total of 864 female sandflies were captured, and 862 (99.8%) were identified as L. intermedia species. However, two unidentified specimens were considered to be part of the genus Lutzomyia. Among the females examined, 396 specimens presented reactions to a certain type of tested antiserum, and most (67.9%) reacted to the simple type. These sandflies fed mainly on the blood of birds, opossums, and rodents, but specimens that fed on the blood of humans, dogs, horses, cattle, and cats were also found. Among the cross-reactions found (32.1%), bird/rodent, bird/opossum, bird/dog, bird/human, and horse/dog cross-reactions were the most common. These results demonstrate a tendency in the eclectic feeding behavior of L. intermedia and support its potential role as a vector for ACL in the study area.

Transcriptome Changes Associated with Anaerobic Growth in Yersinia intermedia (ATCC29909)

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Kiley, Patricia J.; Glasner, Jeremy D.; Perna, Nicole T.

2013-01-01

Background The yersiniae (Enterobacteriaceae) occupy a variety of niches, including some in human and flea hosts. Metabolic adaptations of the yersiniae, which contribute to their success in these specialized environments, remain largely unknown. We report results of an investigation of the transcriptome under aerobic and anaerobic conditions for Y. intermedia, a non-pathogenic member of the genus that has been used as a research surrogate for Y. pestis. Y. intermedia shares characteristics of pathogenic yersiniae, but is not known to cause disease in humans. Oxygen restriction is an important environmental stimulus experienced by many bacteria during their life-cycles and greatly influences their survival in specific environments. How oxygen availability affects physiology in the yersiniae is of importance in their life cycles but has not been extensively characterized. Methodology/Principal Findings Tiled oligonucleotide arrays based on a draft genome sequence of Y. intermedia were used in transcript profiling experiments to identify genes that change expression in response to oxygen availability during growth in minimal media with glucose. The expression of more than 400 genes, constituting about 10% of the genome, was significantly altered due to oxygen-limitation in early log phase under these conditions. Broad functional categorization indicated that, in addition to genes involved in central metabolism, genes involved in adaptation to stress and genes likely involved with host interactions were affected by oxygen-availability. Notable among these, were genes encoding functions for motility, chemotaxis and biosynthesis of cobalamin, which were up-regulated and those for iron/heme utilization, methionine metabolism and urease, which were down-regulated. Conclusions/Significance This is the first transcriptome analysis of a non-pathogenic Yersinia spp. and one of few elucidating the global response to oxygen limitation for any of the yersiniae. Thus this study

Red blood cells free α-haemoglobin pool: a biomarker to monitor the β-thalassemia intermedia variability. The ALPHAPOOL study.

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Vasseur, Corinne; Domingues-Hamdi, Elisa; Ledudal, Katia; Le Corvoisier, Philippe; Barau, Caroline; Ghaleh, Bijan; Rialland, Amandine; Pissard, Serge; Galactéros, Frédéric; Baudin-Creuza, Véronique

2017-10-01

The severity of β-thalassaemia (β-thal) intermedia is mainly correlated to the degree of imbalanced α/non α-globin chain synthesis. The phenotypic diversity of β-thal depends on this imbalance and reflects all possible combinations of α- and β-globin genotypes, levels of fetal haemoglobin (HbF) and co-inheritance of other modulating factors. This study aimed to demonstrate the validity of a new surrogate of α/non α-globin biosynthetic ratio by measuring the soluble α-Hb pool in lysed red blood cells. Our results confirm that the α-Hb pool measurement allows a good discrimination between β-thal intermedia patients, controls and α-thal patients (P intermedia patients and may be clinically useful for monitoring the evolution of the disequilibrium of globin synthesis in response to treatments. © 2017 John Wiley & Sons Ltd.

Intermedia: A System for Linking Multimedia Documents. IRIS Technical Report 86-2.

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Yankelovich, Nicole

"Intermedia" is a hypermedia system which was developed for use in university research and teaching. It provides a framework for object-oriented, direct manipulation editors and applications, and the capability to link together materials created with those applications. Instructors are able to construct exploratory environments for their…

HACIA UN CURRÍCULO FLEXIBLE: SALIDAS INTERMEDIAS Y CURSOS OPTATIVOS

Directory of Open Access Journals (Sweden)

Carlos Ortigoza Garcell

2013-04-01

Full Text Available Tradicionalmente los currículos de las carreras universitarias se han caracterizado por el predominio de una formación de tipo terminal con una sola salida al mercado laboral. Con el fin de contribuir de manera flexible desde la Formación Laboral y Profesional a que los estudiantes definan su trayectoria formativa, en este trabajo se analizan las ventajas de las salidas intermedias en una carrera universitaria, sin afectar la continuidad de los estudios superiores ni la calidad en la formación del egresado universitario. Estas permiten a los estudiantes obtener un título de nivel medio que los pone en condiciones de salir al mercado laboral con un perfil ocupacional relacionado con la carrera universitaria que estudia. Desde la perspectiva curricular la propuesta realizada se basa en currículos flexibles con salidas intermedias donde pueden ser de gran utilidad los cursos optativos y/o electivos.PALABRAS CLAVE: currículo; salidas intermedias; asignaturas optativasTOWARDS A FLEXIBLE CURRICULUM: INTERMEDIATE EXITS AND OPTIONAL COURSES ABSTRACTTraditionally, the curricula of the university degree courses have been focused on the formation of professionals within the required period for their graduation (5-6 years, with a single exit to the labour market. This research is aimed at contributing, from the Labour and Professional Formation of the students, to the definition of their formative process, according to their needs and interests. This paper also analyses the advantages of the intermediate exits in a university degree course, and how they allow students to obtain a college degree without affecting the continuation of the higher education studies or the quality of the formation of the university graduate. Such reality favours the insertion of the students in the labour market under conditions that suit the qualifications and experience related to the degree they are doing. From the curricular perspective, the proposal is based on

Influence of in-feed zinc bacitracin and tiamulin treatment on experimental avian intestinal spirochaetosis caused by Brachyspira intermedia.

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Hampson, D J; Oxberry, S L; Stephens, C P

2002-06-01

Thirty individually caged layer hens were inoculated with Brachyspira intermedia, and 20 control birds remained unchallenged. Birds received a diet containing 100 parts/10(6) zinc bacitracin (ZnB), and were monitored for 10 weeks. B. intermedia was recovered sporadically from five of the inoculated birds, and there were no significant effects on body weight, faecal water or egg production. ZnB was presumed to be indirectly inhibiting spirochaete growth, and when removed from the diet, 18 of the 30 inoculated birds rapidly became culture positive. After 4 weeks, 10 of the 30 infected birds were treated with tiamulin at 25 mg/kg for 5 days, and 10 were returned to the diet containing ZnB. Birds receiving tiamulin became spirochaete negative and maintained their egg production, but re-infection occurred. The other 20 infected birds had a significant drop in egg production, but those receiving ZnB showed a reduced colonization by B. intermedia after 3 weeks.

Bacterial diversity of the American sand fly Lutzomyia intermedia using high-throughput metagenomic sequencing.

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Monteiro, Carolina Cunha; Villegas, Luis Eduardo Martinez; Campolina, Thais Bonifácio; Pires, Ana Clara Machado Araújo; Miranda, Jose Carlos; Pimenta, Paulo Filemon Paolucci; Secundino, Nagila Francinete Costa

2016-08-31

Parasites of the genus Leishmania cause a broad spectrum of diseases, collectively known as leishmaniasis, in humans worldwide. American cutaneous leishmaniasis is a neglected disease transmitted by sand fly vectors including Lutzomyia intermedia, a proven vector. The female sand fly can acquire or deliver Leishmania spp. parasites while feeding on a blood meal, which is required for nutrition, egg development and survival. The microbiota composition and abundance varies by food source, life stages and physiological conditions. The sand fly microbiota can affect parasite life-cycle in the vector. We performed a metagenomic analysis for microbiota composition and abundance in Lu. intermedia, from an endemic area in Brazil. The adult insects were collected using CDC light traps, morphologically identified, carefully sterilized, dissected under a microscope and the females separated into groups according to their physiological condition: (i) absence of blood meal (unfed = UN); (ii) presence of blood meal (blood-fed = BF); and (iii) presence of developed ovaries (gravid = GR). Then, they were processed for metagenomics with Illumina Hiseq Sequencing in order to be sequence analyzed and to obtain the taxonomic profiles of the microbiota. Bacterial metagenomic analysis revealed differences in microbiota composition based upon the distinct physiological stages of the adult insect. Sequence identification revealed two phyla (Proteobacteria and Actinobacteria), 11 families and 15 genera; 87 % of the bacteria were Gram-negative, while only one family and two genera were identified as Gram-positive. The genera Ochrobactrum, Bradyrhizobium and Pseudomonas were found across all of the groups. The metagenomic analysis revealed that the microbiota of the Lu. intermedia female sand flies are distinct under specific physiological conditions and consist of 15 bacterial genera. The Ochrobactrum, Bradyrhizobium and Pseudomonas were the common genera. Our results detailing

Treatment with hydroxyurea in thalassemia intermedia with paravertebral pseudotumors of extramedullary hematopoiesis.

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Cario, H; Wegener, M; Debatin, K-M; Kohne, E

2002-08-01

Excessive ineffective erythropoiesis in thalassemia intermedia may cause paravertebral pseudotumors of extramedullary hematopoiesis. Due to the proximity to the spinal canal, these paravertebral masses carry the risk of severe neurological damage. Treatment strategies include hypertransfusion, radiotherapy, and laminectomy. Hydroxyurea, stimulating fetal hemoglobin synthesis, may represent an alternative therapeutic approach. We report on a 26-year-old patient suffering from thalassemia intermedia with progressive anemia symptoms and presenting multiple intrathoracic paravertebral pseudotumors of extramedullary hematopoiesis. Hypertransfusion therapy and splenectomy were followed by regular transfusion (baseline hemoglobin 10 g/dl) and chelation with desferrioxamine. With this treatment, clinical symptoms disappeared, paravertebral hematopoietic masses did not progress, but severe hemosiderosis developed within a few years. Hydroxyurea therapy was initiated to increase the efficacy of erythropoiesis, thereby reducing the required transfusion volume but suppressing concomitantly further expansion of extramedullary hematopoiesis, and finally leading to a reduction of transfusional iron load. Treatment was started with 4 mg/kg per day and stepwise increased to 12.5 mg/kg per day. The fetal hemoglobin concentration increased from 4.5 to 5.5 g/dl after 1 year and to 9.9 g/dl after 2 years of treatment. The yearly transfusion volume was halved during the 1st year of treatment. At present, after 26 months of treatment, the patient has been transfusion-independent for 10 months. Serum ferritin levels decreased from 2844 to 1335 ng/ml. Size and shape of paravertebral hematopoietic pseudotumors remained stable. No side effects of hydroxyurea have been observed. In thalassemia intermedia patients with extramedullary hematopoiesis, hydroxyurea may lead to independence from regular transfusion therapy without further expansion of ectopic hematopoietic tissue.

Does estradiol have an impact on the dipeptidyl peptidase IV enzyme activity of the Prevotella intermedia group bacteria?

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Fteita, Dareen; Könönen, Eija; Gürsoy, Mervi; Söderling, Eva; Gürsoy, Ulvi Kahraman

2015-12-01

Initiation and development of pregnancy-associated gingivitis is seemingly related to the microbial shift towards specific gram-negative anaerobes in subgingival biofilms. It is known that Prevotella intermedia sensu lato is able to use estradiol as an alternative source of growth instead of vitamin K. The aim of the present study was to investigate the impact of estradiol on the bacterial dipeptidyl peptidase IV (DPPIV) enzyme activity in vitro as a virulent factor of the Prevotella intermedia group bacteria, namely P. intermedia, Prevotella nigrescens, Prevotella pallens, and Prevotella aurantiaca. In all experiments, 2 strains of each Prevotella species were used. Bacteria were incubated with the concentrations of 0, 30, 90, and 120 nmol/L of estradiol and were allowed to build biofilms at an air-solid interface. DPPIV activities of biofilms were measured kinetically during 20 min using a fluorometric assay. The enzyme activity was later related to the amount of protein produced by the same biofilm, reflecting the biofilm mass. Estradiol significantly increased DPPIV activities of the 8 Prevotella strains in a strain- and dose-dependent manner. In conclusion, our in vitro experiments indicate that estradiol regulates the DPPIV enzyme activity of P. intermedia, P. nigrescens, P. pallens, and P. aurantiaca strains differently. Our results may, at least partly, explain the role of estradiol to elicit a virulent state which contributes to the pathogenesis of pregnancy-related gingivitis. Copyright © 2015 Elsevier Ltd. All rights reserved.

Carbon monoxide-releasing molecule-3 suppresses Prevotella intermedia lipopolysaccharide-induced production of nitric oxide and interleukin-1β in murine macrophages.

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Choi, Eun-Young; Choe, So-Hui; Hyeon, Jin-Yi; Choi, Jeom-Il; Choi, In Soon; Kim, Sung-Jo

2015-10-05

This study was performed to analyze the effect of carbon monoxide (CO)-releasing molecule-3 (CORM-3) in alleviating the production of proinflammatory mediators in macrophages treated with lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen associated with periodontal disease, and its possible mechanisms of action. LPS was isolated using the hot phenol-water method. Culture supernatants were assayed for nitric oxide (NO) and interleukin-1β (IL-1β). Gene expression was quantified by real-time PCR, and protein expression by immunoblotting. DNA-binding activities of NF-κB subunits were determined using an ELISA-based kit. CORM-3 suppressed the production of inducible NO synthase (iNOS)-derived NO and IL-1β at both gene transcription and translation levels in P. intermedia LPS-activated RAW264.7 cells. CORM-3 enhanced heme oxygenase-1 (HO-1) expression in cells stimulated with P. intermedia LPS, and inhibition of HO-1 activity by SnPP notably reversed the suppressive effect of CORM-3 on LPS-induced production of NO. LPS-induced phosphorylation of p38 and JNK was not affected by CORM-3. CORM-3 did not influence P. intermedia LPS-induced degradation of IκB-α. Instead, nuclear translocation of NF-κB p65 and p50 subunits was blocked by CORM-3 in LPS-treated cells. In addition, CORM-3 reduced LPS-induced p65 and p50 binding to DNA. Besides, CORM-3 significantly suppressed P. intermedia LPS-induced phosphorylation of STAT1. Overall, this study indicates that CORM-3 suppresses the production of NO and IL-1β in P. intermedia LPS-activated murine macrophages via HO-1 induction and inhibition of NF-κB and STAT1 pathways. The modulation of host inflammatory response by CORM-3 would be an attractive therapeutic approach to attenuate the progression of periodontal disease. Copyright © 2015 Elsevier B.V. All rights reserved.

Ruptured Massa Intermedia Secondary to Hydrocephalus.

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El Damaty, Ahmed; Langner, Soenke; Schroeder, Henry W S

2017-01-01

We report a case of ruptured massa intermedia (MI) as a sequela of hydrocephalus. A single case report is presented describing the sequelae of tumor bed hematoma after a posterior fossa hemangioblastoma resection in which the patient bled 3 days after surgery, resulting in secondary hydrocephalus and subsequently dilatation of the third ventricle, which resulted in rupture of the MI. The patient was managed on emergency basis with an external ventricular drain then endoscopically with a third ventriculostomy and clot extraction. Absent MI is not uncommon in hydrocephalic patients, and it is assumed to be the result of rupture from acute dilatation of the third ventricle. Our case report proves this assumption and documents the presence and absence of the MI before and after developing hydrocephalus. Copyright © 2016 Elsevier Inc. All rights reserved.

Gravi-photomorphogenesis of the moss Pottia intermedia protonemata

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Demkiv, O. T.; Kyjak, N. Y.; Khorkavtsiv, Y. D.; Kit, N. A.

The protonemata development proceeds in the process of gradual differentiation of growing apical cells and intercalar cells the shortened lateral branches of the latters being transformed into three-dimensional gametophore buds (Demkiv et al., 1991). Normal course of plant development needs favourable external conditions. Sometimes, however, external environment agents can accelerate the development of organism. So, apical protonema cells of darkgrown gravitropic P. intermedia differentiate gametophore-buds in light of low intensity (Ripetskyj, 1999). We investigate the influence of gravistimulation on bud formation in haploid and diploid P. intermedia protonema. Diploid protonema was found to react on light weaker than haploid one. Under the influence of light the darkgrown apical cells and lateral branches of haploid protonema were directly transformed into buds, while in diploid protonema at first the formation of bundles of rhizoid type filaments takes place on the tips of caulonema and buds appeared in center of such bundles. The participation of gravity in gametophore bud formation was assessed by clinorotating protonema in darkness. Being illuminated such protonema also developed buds quickly the latters being formed along all stolon. It can be suggested that at 1g the growth zone of apical cells actively attract inductors of bud formation. During clinorotation the inductors probably are transferred much more slower than under stationary state and that is why the buds arised not only at the tips of stolons but along all their length. It is known that gametophore bud formation can be stimulated by exogenous phytohormones. As M. Bopp (1980) has shown, that kinetin selectively promotes bud formation on moss protonema. Our observations have shown 0,5 -- 50 μ M of kinetin stimulate the bud formation on diploid aposporic protonema much more effectively that on haploid one. It can be concluded that the amount of endogenous cytokinins in haploid protonemal cells is

Biology of the first generation of a laboratory colony of Nyssomyia intermedia (Lutz & Neiva, 1912) and Nyssomyia neivai (Pinto, 1926) (Diptera: Psychodidae).

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Andrade Filho, José Dilermando; Galati, Eunice A Bianchi; Falcão, Alda Lima

2004-10-01

The phlebotomine sand flies Nyssomyia intermedia (Lutz & Neiva, 1912) and Nyssomyia neivai (Pinto, 1926) are very close and may be involved in the transmission of Leishmania spp. Ross, 1903 in Brazil. The biology of the first laboratory-reared generations of these species, descended from insects captured in Além Paraíba (N. intermedia) and Corinto (N. neivai) in the Brazilian state of Minas Gerais, is described here. The captured females were fed on hamsters and maintained individually in rearing pots. Laboratory temperature and relative humidity were maintained at 25-26 masculineC and 80% respectively. The productivity of the first generation of N. intermedia was greater than that of N. neivai, and its development time clearly shorter, particularly for the second and third larval instars.

Cloning of a sesquiterpene synthase from Lavandula x intermedia glandular trichomes.

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Sarker, Lukman S; Demissie, Zerihun A; Mahmoud, Soheil S

2013-11-01

The essential oil (EO) of Lavandula is dominated by monoterpenes, but can also contain small amounts of sesquiterpenes, depending on species and environmental conditions. For example, the sesquiterpene 9-epi-caryophyllene can make up to 8 % of the EO in a few species, including those commercially propagated for EO production. Here, we report the cloning and functional characterization of 9-epi-caryophyllene synthase (LiCPS) from the glandular trichomes of Lavandula x intermedia, cv. Grosso. The 1,617 bp open reading frame of LiCPS, which did not encode a transit peptide, was expressed in Escherichia coli and the recombinant protein purified by Ni-NTA agarose affinity chromatography. The ca. 60 kDa recombinant protein specifically converted farnesyl diphosphate to 9-epi-caryophyllene. LiCPS also produced a few monoterpenes when assayed with the monoterpene precursor geranyl diphosphate (GPP), but--unlike most monoterpene synthases--was not able to derive detectable amounts of any products from the cis isomer of GPP, neryl diphosphate. The LiCPS transcripts accumulated in developing L. x intermedia flowers and were highly enriched in glandular trichomes, but were not detected in leaves suggesting that the transcriptional expression of this gene is spatially and developmentally regulated.

NCX 4040, a nitric oxide-donating aspirin derivative, inhibits Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.

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Choi, Eun-Young; Choe, So-Hui; Hyeon, Jin-Yi; Park, Hae Ryoun; Choi, Jeom-Il; Choi, In Soon; Kim, Sung-Jo

2015-12-05

In this study, the effects and underlying mechanisms of NCX 4040, a nitric oxide (NO)-donating aspirin derivative, on the production of proinflammatory mediators were examined using murine macrophages exposed to lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in the etiology of periodontal disease. NCX 4040 significantly reduced P. intermedia LPS-induced production of inducible NO synthase (iNOS)-derived NO, IL-1β and IL-6 as well as their mRNA expression in RAW264.7 cells. Notably, NCX 4040 was much more effective than the parental compound aspirin in reducing LPS-induced production of inflammatory mediators. NCX 4040 induced the expression of heme oxygenase-1 (HO-1) in cells treated with P. intermedia LPS, and the suppressive effect of NCX 4040 on LPS-induced NO production was significantly reversed by SnPP, a competitive HO-1 inhibitor. NCX 4040 did not influence LPS-induced phosphorylation of JNK and p38. IκB-α degradation as well as nuclear translocation and DNA-binding activities of NF-κB p65 and p50 subunits induced by P. intermedia LPS were significantly reduced by NCX 4040. Besides, LPS-induced phosphorylation of STAT1 and STAT3 was significantly down-regulated by NCX 4040. Further, NCX 4040 elevated the SOCS1 mRNA in cells stimulated with LPS. This study indicates that NCX 4040 inhibits P. intermedia LPS-induced production of NO, IL-1β and IL-6 in murine macrophages through anti-inflammatory HO-1 induction and suppression of NF-κB, STAT1 and STAT3 activation, which is associated with the activation of SOCS1 signaling. NCX 4040 could potentially be a promising tool in the treatment of periodontal disease, although further studies are required to verify this. Copyright © 2015 Elsevier B.V. All rights reserved.

Complete genome sequence of Brachyspira intermedia reveals unique genomic features in Brachyspira species and phage-mediated horizontal gene transfer

Science.gov (United States)

2011-01-01

Background Brachyspira spp. colonize the intestines of some mammalian and avian species and show different degrees of enteropathogenicity. Brachyspira intermedia can cause production losses in chickens and strain PWS/AT now becomes the fourth genome to be completed in the genus Brachyspira. Results 15 classes of unique and shared genes were analyzed in B. intermedia, B. murdochii, B. hyodysenteriae and B. pilosicoli. The largest number of unique genes was found in B. intermedia and B. murdochii. This indicates the presence of larger pan-genomes. In general, hypothetical protein annotations are overrepresented among the unique genes. A 3.2 kb plasmid was found in B. intermedia strain PWS/AT. The plasmid was also present in the B. murdochii strain but not in nine other Brachyspira isolates. Within the Brachyspira genomes, genes had been translocated and also frequently switched between leading and lagging strands, a process that can be followed by different AT-skews in the third positions of synonymous codons. We also found evidence that bacteriophages were being remodeled and genes incorporated into them. Conclusions The accessory gene pool shapes species-specific traits. It is also influenced by reductive genome evolution and horizontal gene transfer. Gene-transfer events can cross both species and genus boundaries and bacteriophages appear to play an important role in this process. A mechanism for horizontal gene transfer appears to be gene translocations leading to remodeling of bacteriophages in combination with broad tropism. PMID:21816042

Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan.

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Gul, Rahman; Jan, Syed Umer; Faridullah, Syed; Sherani, Samiullah; Jahan, Nusrat

2017-01-01

The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the E. intermedia to prove its uses in Pakistani folk medicines for the treatment of asthma and bronchitis. Antioxidant activity was analyzed by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. Standard methods were used for the identification of cardiac glycosides, phenolic compounds, flavonoids, anthraquinones, and alkaloids. High performance liquid chromatography (HPLC) was used for quantitative purpose of ephedrine alkaloids in E. intermedia . The quantitative separation was confirmed on Shimadzu 10AVP column (Shampack) of internal diameter (id) 3.0 mm and 50 mm in length. The extract of the solute in flow rate of 1 ml/min at the wavelength 210 nm and methanolic extract showed the antioxidant activity and powerful oxygen free radicals scavenging activities and the IC50 for the E. intermedia plant was near to the reference standard ascorbic acid. The HPLC method was useful for the quantitative purpose of ephedrine (E) and pseudoephedrine (PE) used for 45 samples of one species collected from central habitat in three districts (Ziarat, Shairani, and Kalat) of Balochistan. Results showed that average alkaloid substance in E. intermedia was as follows: PE (0.209%, 0.238%, and 0.22%) and E (0.0538%, 0.0666%, and 0.0514%).

THALASSAEMIA INTERMEDIA : AN UPDATE

Directory of Open Access Journals (Sweden)

Maria Domenica Cappellini

2009-06-01

Full Text Available

Our understanding of the molecular and pathophysiological mechanisms underlying the disease process in patients with thalassaemia intermedia (TI has substantially increased over the past decade. TI encompasses a wide clinical spectrum of beta-thalassaemia phenotypes. Some TI patients are asymptomatic until adult life, whereas others are symptomatic from as young as 2 years of age. A number of clinical complications commonly associated with TI are rarely seen in thalassaemia major, including extramedullary hematopoiesis, leg ulcers, gallstones, thrombosis and pulmonary hypertension. There are a number of options currently available for managing patients with TI, including transfusion therapy, iron chelation therapy, modulation of foetal haemoglobin production and haematopoietic stem cell transplantation. However, at present, there are no clear guidelines for an orchestrated optimal treatment plan.

Networked Intermedia Agenda Setting: The Geography of a Hyperlinked Scandinavian News Ecology

DEFF Research Database (Denmark)

Sjøvaag, Helle; Stavelin, Eirik; Karlsson, Michael

How does agenda setting work within the hyperlinked Scandinavian news ecology? This paper investigates intermedia agenda setting within and between the local, regional, national and supra-national levels in Sweden, Denmark and Norway; analyses the center/periphery dimensions of hyperlink connecti......, social geography and hyperlinked news agendas in Scandinavia, adding to the research on the political implications of the Internet on national public spheres....... March 2016, amounting to approximately 2 million hyperlinks, each geotagged with publication origin. The visualisation of the hyperlink structure is one of the main results of the analysis, illuminating a) the relative disconnect between local and national hyperlinked agendas, b) the relative disconnect...... between news agendas in the three countries, and c) the connectedness enabled by size, resources and central location in the Scandinavian hyperlinked information structure. The network analysis provides new insights into the relationship between centralized political structures, media ownership dispersal...

Biology of the first generation of a laboratory colony of Nyssomyia intermedia (Lutz & Neiva, 1912 and Nyssomyia neivai (Pinto, 1926 (Diptera: Psychodidae

Directory of Open Access Journals (Sweden)

José Dilermando Andrade Filho

2004-10-01

Full Text Available The phlebotomine sand flies Nyssomyia intermedia (Lutz & Neiva, 1912 and Nyssomyia neivai (Pinto, 1926 are very close and may be involved in the transmission of Leishmania spp. Ross, 1903 in Brazil. The biology of the first laboratory-reared generations of these species, descended from insects captured in Além Paraíba (N. intermedia and Corinto (N. neivai in the Brazilian state of Minas Gerais, is described here. The captured females were fed on hamsters and maintained individually in rearing pots. Laboratory temperature and relative humidity were maintained at 25-26ºC and 80% respectively. The productivity of the first generation of N. intermedia was greater than that of N. neivai, and its development time clearly shorter, particularly for the second and third larval instars.

Mycelial pellet formation by edible ascomycete filamentous fungi, Neurospora intermedia.

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Nair, Ramkumar B; Lennartsson, Patrik R; Taherzadeh, Mohammad J

2016-12-01

Pellet formation of filamentous fungi in submerged culture is an imperative topic of fermentation research. In this study, we report for the first time the growth of filamentous ascomycete fungus, Neurospora intermedia in its mycelial pellet form. In submerged culture, the growth morphology of the fungus was successfully manipulated into growing as pellets by modifying various cultivation conditions. Factors such as pH (2.0-10.0), agitation rate (100-150 rpm), carbon source (glucose, arabinose, sucrose, and galactose), the presence of additive agents (glycerol and calcium chloride) and trace metals were investigated for their effect on the pellet formation. Of the various factors screened, uniform pellets were formed only at pH range 3.0-4.0, signifying it as the most influential factor for N. intermedia pellet formation. The average pellet size ranged from 2.38 ± 0.12 to 2.86 ± 0.38 mm. The pellet formation remained unaffected by the inoculum type used and its size showed an inverse correlation with the agitation rate of the culture. Efficient glucose utilization was observed with fungal pellets, as opposed to the freely suspended mycelium, proving its viability for fast-fermentation processes. Scale up of the pelletization process was also carried out in bench-scale airlift and bubble column reactors (4.5 L).

Design for safety : A new service for alarming and informing the population in case of emergency

NARCIS (Netherlands)

Jagtman, H.M.

2012-01-01

In case of emergencies the population in danger should be alarmed so individuals can take action to get or remain out of danger. For alarming the population the means available are limited. Many countries have outdoor sirens. They operability however is limited since the siren has only one implicit

Polymorphism for pKALILO based senescence in Hawaiian populations of Neurospora intermedia and Neurospora tetrasperma.

NARCIS (Netherlands)

Maas, M.F.P.M.; Mourik, van A.; Hoekstra, R.F.; Debets, A.J.M.

2005-01-01

The natural population of Neurospora intermedia from Hawaii is polymorphic for the presence of the linear mitochondrial plasmid pKALILO that is associated with an infectious senescence syndrome. Although inter-specific horizontal transmission is experimentally possible, thus far pKALILO associated

Approaches to management of beta-thalassemia intermedia

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Joseph E. Maakaron

2013-03-01

Full Text Available Thalassemia intermedia is a genetically diverse group of diseases that is the result of an imbalance in the production of the alpha and beta chains with ensuing chronic hemolysis, ineffective erythropoiesis, and iron overload.Resulting complications include bone changes, hypercoagulability, and end-organ damage due to iron overload. This decade has witnessed major breakthroughs in the management of thalassemia. In this article, we examine these novelties in therapy including iron chelation therapy, stem cell transplant, and gene therapy.Iron chelation therapy has been revolutionized with the advent of deferasirox, a once-daily oral iron chelator, that has been shown to be safe and efficacious.Gene therapy was also at the core of this revolution with the discovery of novel gene elements and viral vectors allowing for better control and improved outcomes.

Intermedin A, a New Labdane Diterpene Isolated from Alpinia intermedia, Prolonged the Survival Time of P-388D1 Tumor-Bearing CDF1 Mice.

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Chen, Lih-Geeng; Su, Pei-Jung; Tsai, Po-Wei; Yang, Ling-Ling; Wang, Ching-Chiung

2017-01-01

Eight ethanolic extracts of indigenous Taiwanese plants of the genus Alpinia were tested for tumor cytotoxicity against AGS, Hep G2, HeLa, KB, and HL-60 cells. Among the 50 % and 95 % EtOH extracts of eight Alpinia species, the cytotoxic effects of Alpinia intermedia leaves were the strongest. When the leaf extract of A. intermedia was partitioned using n -hexane and aqueous solvents, the n -hexane layer showed a greater cytotoxic effect and could prolong the survival time of P-388D 1 tumor-bearing CDF1 mice. Two new labdane diterpene derivatives, intermedin A ( 1 ) and intermedin B ( 2 ), and coronarin E ( 3 ) were isolated from the n -hexane layer of A. intermedia . Intermedin A induced apoptosis in HL-60 cells at 30 µg/mL and significantly prolonged the survival time of P-388D 1 tumor-bearing CDF 1 mice by 48.7 % at 20 mg/kg of body weight. We suggest that intermedin A is a major compound of A. intermedia and has a cytotoxic effect on HL-60 and P-388D 1 cells. Georg Thieme Verlag KG Stuttgart · New York.

Kinetics activity of Yersinia Intermedia Against ZnO Nanoparticles Either Synergism Antibiotics by Double-Disc Synergy Test Method.

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Fathi Azar Khavarani, Motahareh; Najafi, Mahla; Shakibapour, Zahra; Zaeifi, Davood

2016-03-01

Bacterial resistance to the commonly used antibacterial agents is an increasing challenge in the medicine, and a major problem for the health care systems; the control of their spread is a constant challenge for the hospitals. In this study, we have investigated the antimicrobial activity of the Zinc Oxide nanoparticles against clinical sample; Yersinia intermedia bacteria. Nanoparticle susceptibility constants and death kinetic were used to evaluate the antimicrobial characteristics of the Zinc Oxide (ZnO) against the bacteria. Antimicrobial tests were performed with 10 8 cfu.mL -1 at baseline. At first, Minimum Inhibitory Concentration (MIC) of ZnO was determined and then nanoparticle suspension at one and two times of the MIC was used for death kinetic and susceptibility constant assay at 0 to 360 min treatment time. ZnO nanoparticles with size ranging from 10 to 30 nm showed the highest susceptibility reaction against Y. intermedia (Z=39.06 mL.μg -1 ). The process of Y. intermedia death in ZnO suspension was assumed to follow the first-order kinetics and the survival ratio of bacteria decreased with the increasing treatment time. An increased concentration of the nanoparticle was seen to enhance the bactericidal action of the nanoparticle. Then we performed the best ratio of the nanoparticles on semi-sensitive and resistance antibiotic for the bacteria. However, based on experimental results, synergy of ZnO nanoparticles and Oxacilin was determined and Y. intermedia showed a higher sensitivity compared to the ZnO nanoparticles alone. The results of the present study illustrates that ZnO has a strong antimicrobial effect and could potentially be employed to aid the bacterial control. It could also improve- antibacterial effects in combination with the antibiotics.

Biocidal Potential and Chemical Composition of Industrial Essential Oils from Hyssopus officinalis, Lavandula × intermedia var. Super, and Santolina chamaecyparissus.

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Ortiz de Elguea-Culebras, Gonzalo; Sánchez-Vioque, Raúl; Berruga, María Isabel; Herraiz-Peñalver, David; González-Coloma, Azucena; Andrés, María Fé; Santana-Méridas, Omar

2018-01-01

This work presents the biocidal (insecticidal, ixodicidal, nematicidal, and phytotoxic) effects and chemical compositions of three essential oils obtained from the industrial steam distillation (IEOs) of hyssop (Hyssopus officinalis L.), lavandin (Lavandula × intermedia or L. × hybrida var. Super), and cotton lavender (Santolina chamaecyparissus L.). Their chemical composition analyzed by gas chromatography coupled to mass spectrometry showed 1,8-cineole (53%) and β-pinene (16%) as the major components of H. officinalis, linalyl acetate (38%) and linalool (29%) of L. × intermedia; and 1,8-cineole (10%) and 8-methylene-3-oxatricyclo[5.2.0.0 2,4 ]nonane (8%) in S. chamaecyparissus. The biocidal tests showed that L. × intermedia IEO was the most active against the insect Spodoptera littoralis and toxic to the tick Hyalomma lusitanicum, IEO of H. officinalis was strongly active against S. littoralis, and finally, S. chamaecyparissus IEO was a strong antifeedant against the aphid Rhopalosiphum padi, toxic to H. lusitanicum and with moderate effects against Leptinotarsa decemlineata, S. littoralis, and Lolium perenne. © 2018 Wiley-VHCA AG, Zurich, Switzerland.

Dispersal and survival of Nyssomyia intermedia and Nyssomyia neivai (Diptera: Psychodidae: Phlebotominae) in a cutaneous leishmaniasis endemic area of the speleological province of the Ribeira Valley, state of São Paulo, Brazil.

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Galati, Eunice A B; Fonseca, Mauro Breviglieri; Marassá, Ana Maria; Bueno, Edna Fátima M

2009-12-01

The dispersal and survival of the phlebotomines Nyssomyia intermedia and Nyssomyia neivai (both implicated as vectors of the cutaneous leishmaniasis agent) in an endemic area was investigated using a capture-mark-release technique in five experiments from August-December 2003 in municipality of Iporanga, state of São Paulo, Brazil. A total of 1,749 males and 1,262 females of Ny. intermedia and 915 males and 411 females of Ny. neivai were marked and released during the five experiments. Recapture attempts were made using automatic light traps, aspiration in natural resting places and domestic animal shelters and Shannon traps. A total of 153 specimens (3.48%) were recaptured: 2.59% (78/3,011) for Ny. intermedia and 5.35% (71/1,326) for Ny. neivai. Both species were recaptured up to 144 h post-release, with the larger part of them recaptured within 48 h. The median dispersion distances for Ny. intermedia and Ny. neivai, respectively, were 109 m and 100 m. The greatest dispersal range of Ny. intermedia was 180 m, while for Ny. neivai one female was recaptured in a pasture at 250 m and another in a pigsty at 520 m, showing a tendency to disperse to more open areas. The daily survival rates calculated based on regressions of the numbers of marked insects recaptured on the six successive days after release were 0.746 for males and 0.575 for females of Ny. intermedia and 0.649 for both sexes of Ny. neivai. The size of the populations in the five months ranged from 8,332-725,085 for Ny. intermedia males, 2,193-104,490 for Ny. intermedia females, 1,687-350,122 for Ny. neivai males and 254-49,705 for Ny. neivai females.

Interaction of Prevotella intermedia strain 17 leucine-rich repeat domain protein AdpF with eukaryotic cells promotes bacterial internalization.

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Sengupta, Dipanwita; Kang, Dae-Joong; Anaya-Bergman, Cecilia; Wyant, Tiana; Ghosh, Arnab K; Miyazaki, Hiroshi; Lewis, Janina P

2014-06-01

Prevotella intermedia is an oral bacterium implicated in a variety of oral diseases. Although internalization of this bacterium by nonphagocytic host cells is well established, the molecular players mediating the process are not well known. Here, the properties of a leucine-rich repeat (LRR) domain protein, designated AdpF, are described. This protein contains a leucine-rich region composed of 663 amino acid residues, and molecular modeling shows that it folds into a classical curved solenoid structure. The cell surface localization of recombinant AdpF (rAdpF) was confirmed by electron and confocal microscopy analyses. The recombinant form of this protein bound fibronectin in a dose-dependent manner. Furthermore, the protein was internalized by host cells, with the majority of the process accomplished within 30 min. The internalization of rAdpF was inhibited by nystatin, cytochalasin, latrunculin, nocodazole, and wortmannin, indicating that microtubules, microfilaments, and signal transduction are required for the invasion. It is noteworthy that preincubation of eukaryotic cells with AdpF increased P. intermedia 17 internalization by 5- and 10-fold for HeLa and NIH 3T3 fibroblast cell lines, respectively. The addition of the rAdpF protein was also very effective in inducing bacterial internalization into the oral epithelial cell line HN4, as well as into primary cells, including human oral keratinocytes (HOKs) and human umbilical vein endothelial cells (HUVECs). Finally, cells exposed to P. intermedia 17 internalized the bacteria more readily upon reinfection. Taken together, our data demonstrate that rAdpF plays a role in the internalization of P. intermedia 17 by a variety of host cells.

Prevotella intermedia induces severe bacteremic pneumococcal pneumonia in mice with upregulated platelet-activating factor receptor expression.

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Nagaoka, Kentaro; Yanagihara, Katsunori; Morinaga, Yoshitomo; Nakamura, Shigeki; Harada, Tatsuhiko; Hasegawa, Hiroo; Izumikawa, Koichi; Ishimatsu, Yuji; Kakeya, Hiroshi; Nishimura, Masaharu; Kohno, Shigeru

2014-02-01

Streptococcus pneumoniae is the leading cause of respiratory infection worldwide. Although oral hygiene has been considered a risk factor for developing pneumonia, the relationship between oral bacteria and pneumococcal infection is unknown. In this study, we examined the synergic effects of Prevotella intermedia, a major periodontopathic bacterium, on pneumococcal pneumonia. The synergic effects of the supernatant of P. intermedia (PiSup) on pneumococcal pneumonia were investigated in mice, and the stimulation of pneumococcal adhesion to human alveolar (A549) cells by PiSup was assessed. The effects of PiSup on platelet-activating factor receptor (PAFR) transcript levels in vitro and in vivo were analyzed by quantitative real-time PCR, and the differences between the effects of pneumococcal infection induced by various periodontopathic bacterial species were verified in mice. Mice inoculated with S. pneumoniae plus PiSup exhibited a significantly lower survival rate, higher bacterial loads in the lungs, spleen, and blood, and higher inflammatory cytokine levels in the bronchoalveolar lavage fluid (macrophage inflammatory protein 2 and tumor necrosis factor alpha) than those infected without PiSup. In A549 cells, PiSup increased pneumococcal adhesion and PAFR transcript levels. PiSup also increased lung PAFR transcript levels in mice. Similar effects were not observed in the supernatants of Porphyromonas gingivalis or Fusobacterium nucleatum. Thus, P. intermedia has the potential to induce severe bacteremic pneumococcal pneumonia with enhanced pneumococcal adhesion to lower airway cells.

Fatty acid methyl ester from Neurospora intermedia N-1 isolated from Indonesian red peanut cake (oncom merah).

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Priatni, S; Hartati, S; Dewi, P; Kardono, L B S; Singgih, M; Gusdinar, T

2010-08-01

The objective of this study was to identify the Fatty Acid Methyl Ester (FAME) from Neurospora intermedia N-1 that isolated from Indonesian red peanut cake (oncom). FAME profiles have been used as biochemical characters to study many different groups of organisms, such as bacteria and yeasts. FAME from N. intermedia N-1 was obtained by some stages of extraction the orange spores and fractination using a chromatotron. The pure compound (1) was characterized by 500 mHz NMR (1H and 13C), FTIR and LC-MS. Summarized data's of 1H and 13C NMR spectra of compound 1 contained 19 Carbon, 34 Hydrogen and 2 Oxygen (C19H34O2). The position of the double bonds at carbon number 8 and 12 were indicated in the HMBC spectrum (2D-NMR). LC-MS spectrum indicates molecular weight of the compound 1 as 294 which is visible by the presence of protonated molecular ion [M+H] at m/z 295. Methyl esters of long chain fatty acids was presented by a 3 band pattern of IR spectrum with bands near 1249, 1199 and 1172 cm(-1). We suggested that the structure of the pure compound 1 is methyl octadeca-8,12-dienoate. The presence methyl octadeca-8,12-dienoate in N. intermedia is the first report.

Human dental stem cells suppress PMN activity after infection with the periodontopathogens Prevotella intermedia and Tannerella forsythia

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Hieke, Cathleen; Kriebel, Katja; Engelmann, Robby; Müller-Hilke, Brigitte; Lang, Hermann; Kreikemeyer, Bernd

2016-01-01

Periodontitis is characterized by inflammation associated with the colonization of different oral pathogens. We here aimed to investigate how bacteria and host cells shape their environment in order to limit inflammation and tissue damage in the presence of the pathogen. Human dental follicle stem cells (hDFSCs) were co-cultured with gram-negative P. intermedia and T. forsythia and were quantified for adherence and internalization as well as migration and interleukin secretion. To delineate hDFSC-specific effects, gingival epithelial cells (Ca9-22) were used as controls. Direct effects of hDFSCs on neutrophils (PMN) after interaction with bacteria were analyzed via chemotactic attraction, phagocytic activity and NET formation. We show that P. intermedia and T. forsythia adhere to and internalize into hDFSCs. This infection decreased the migratory capacity of the hDFSCs by 50%, did not disturb hDFSC differentiation potential and provoked an increase in IL-6 and IL-8 secretion while leaving IL-10 levels unaltered. These environmental modulations correlated with reduced PMN chemotaxis, phagocytic activity and NET formation. Our results suggest that P. intermedia and T. forsythia infected hDFSCs maintain their stem cell functionality, reduce PMN-induced tissue and bone degradation via suppression of PMN-activity, and at the same time allow for the survival of the oral pathogens. PMID:27974831

Spinal cord compression due to extramedullary hematopoiesis in beta-thalassemia intermedia

International Nuclear Information System (INIS)

Munn, Rita K.; Kramer, Carol A.; Arnold, Susanne M.

1998-01-01

Background: Extramedullary hematopoiesis (EMH) occurs in many disorders, including thalassemias and other hemoglobinopathies, and commonly presents in the spleen and liver. We present a case of spinal cord compression in a patient with beta-thalassemia intermedia, and review the literature and available treatment options. Patient and Methods: A 35-year-old black female with beta-thalassemia intermedia presented with a 3-week history of back pain and lower extremity weakness. Neurologic examination was consistent with spinal cord compression, and gadolinium enhanced magnetic resonance imaging (MRI) confirmed this diagnosis. She was given intravenous steroids and radiotherapy was begun in 200 cGy fractions to a total dose of 2000 cGy. Results: At the completion of radiotherapy the patient was ambulatory with mild residual weakness. MRI scans 16 months later showed smaller, but persistent masses, and she remains asymptomatic 5 years from her diagnosis. Conclusion: Recognition of spinal cord EMH requires prompt physical examination and MRI for accurate diagnosis. EMH can be managed with radiation, surgery, transfusions, or a combination of these therapies. Radiation in conservative doses of (750-3500 cGy) is non-invasive, avoids the surgical risks of potentially severe hemorrhage and incomplete resection, and has a high complete remission rate in the majority of patients. Relapse rates are moderate (37.5%), but retreatment provides excellent chance for second remission

Isolation of Yersinia enterocolitica and Y. intermedia from fatal cases of diarrhoeal illness in Bangladesh

NARCIS (Netherlands)

Butler, T.; Islam, M.; Islam, M. R.; Azad, A. K.; Huq, M. I.; Speelman, P.; Roy, S. K.

1984-01-01

From three fatal cases of diarrhoeal illness in Bangladesh, Yersinia species were isolated from tissues at post-mortem examination. One patient was infected with Y. enterocolitica serotype 0:7, 8 and two patients were infected with Y. intermedia. These patients were infected also with other enteric

The production of 7-methyljuglone, plumbagin and quercetin in wild and cultivated Drosera rotundifolia and Drosera intermedia

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B. Baranyai

2016-09-01

Full Text Available The recent establishment of Sphagnum farming areas has created large artificial habitats where Drosera grows under semi-natural conditions. Here we test the suitability, for pharmaceutical purposes, of two Drosera species collected from such areas. We measured the concentration of the biologically active compounds 7-methyljuglone, plumbagin and quercetin in Drosera rotundifolia and D. intermedia. All three compounds were found in pharmacologically suitable concentrations with 7-methlyjuglone characteristic for D. rotundifolia and plumbagin for D. intermedia. The concentrations required for pharmacological purposes were achieved within one year, but higher concentrations occurred in older plants and plants in flower. Concentrations did not differ between plants collected in the morning and in the afternoon. Drosera plants cultivated under semi-natural conditions are suitable as sources of raw materials for industrial pharmacological applications.

Mechanics of lung ventilation in a large aquatic salamander, siren lacertina

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Brainerd; j

1998-06-01

Lung ventilation in Siren lacertina was studied using X-ray video, measurements of body cavity pressure and electromyography of hypaxial muscles. S. lacertina utilizes a two-stroke buccal pump in which mixing of expired and inspired gas is minimized by partial expansion of the buccal cavity during exhalation and then full expansion after exhalation is complete. Mixing is further reduced by the use of one or two accessory inspirations after the first, mixed-gas cycle. Exhalation occurs in two phases: a passive phase in which hydrostatic pressure and possibly lung elasticity force air out of the lungs, and an active phase in which contraction of the transverse abdominis (TA) muscle increases body cavity pressure and forces most of the remaining air out. In electromyograms of the lateral hypaxial musculature, the TA became active 200-400 ms before the rise in body cavity pressure, and activity ceased at peak pressure. The TA was not active during inspiration, and no consistent activity during breathing was noted in the external oblique, internal oblique and rectus abdominis muscles. The finding that the TA is the primary expiratory muscle in S. lacertina agrees with findings in a previous study of another salamander, Necturus maculosus. Together, these results indicate that the use of the TA for exhalation is a primitive character for salamanders and support the hypothesis that the breathing mechanism of salamanders represents an intermediate step in evolution between a buccal pump, in which only head muscles are used for ventilation, and an aspiration pump, in which axial muscles are used for both exhalation and inhalation.

Paraspinal extramedullary hematopoiesis in patients with thalassemia intermedia.

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Haidar, Rachid; Mhaidli, Hani; Taher, Ali T

2010-06-01

Ineffective erythropoiesis in patients with thalassemia intermedia drives extramedullary hematopoietic tumor formation in several parts of the body. Paraspinal involvement has received increasing attention due to the associated morbidity secondary to spinal cord compression. Although the history and physical examination may help narrow the differential diagnosis, radiographic imaging remains essential to confirm the existence of hematopoietic tissue. Characteristic appearance has been observed mainly on magnetic resonance imaging. Several treatment options have been described, including transfusion therapy, laminectomy, radiotherapy, and the use of fetal hemoglobin inducing agents that decrease the hematopoietic drive. However, the ideal management scheme remains controversial. Until large prospective trials evaluate the efficacy and safety of the available treatment options, both in single and in combination therapy, an individualized approach should be entertained.

Heuristic model of education and a prototype of a system for remote siren activation in emergency

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Paun Josif Bereš

2013-02-01

Full Text Available A heuristic models of methodical innovation with special emphasis on training of team members to work in emergency situations, where each team member must be aware of the importance of their work, must know and apply a methodology for finding the best solutions and feel satisfaction with the results as an individual and as a part of that team, are proposed in this paper. Heuristic approach to the problems of teaching in emergency situations should allow team members to come up with the creative process of acquiring knowledge, to learn to think and develop skills for education. It is, therefore, such an approach that is not based on passive observation of phenomena and impersonation performed by a teacher, but establishes an active relationship to phenomena of thought and introduces the team members in their own research of the heuristic concept problems with the range of solutions - in this case, " Digital Communications - a program of operational functions of mobile phones ", the function of emergency situations, the example of a prototype system for automatic activation of the alert - sirens.

Alternative substrata for xaxim on culture of Cattleya intermedia X Hadrolaelia purpurata (Orchidaceae / Substratos alternativos ao xaxim no cultivo do híbrido Cattleya intermedia X Hadrolaelia purpurata (Orchidaceae

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Maria Aparecida da Fonseca Sorace

2009-12-01

Full Text Available The present work had the purpose of evaluating the efficiency of different substrata by vegetal origin, to grow species of orchids (Orchidaceae. Seedlings of Cattleya intermedia X Hadrolaelia purpurata, obtained by in vitro culture, and acclimated, with medium height of 5 cm, had the following substrata: 1- Defibered xaxim (control; 2- Carbonized rice husk; 3- Coconut fiber; 4- Husk pínus; 5- Carbonized rice husk + Coconut fiber (1:1; 6- Carbonized rice husk + Husk pínus (1:1; 7- Coconut fiber + Husk pinus (1:1; 8- Husk pínus + Carbonized rice husk + Coconut fiber (1:1:1. A randomized design with eight treatments and ten replications. Results were submited to Variance Analysis and Tukey Test at 5 %. Growing parameters evaluated after 17 months were: root length, roots number, height of above ground, buds number, leaves number, leaf length, leaf width, total fresh mass and root volume. Determinated substrata parameters were: pH, eletric conductivity and density. All studied substratum can be recommended to substitute xaxim but, coconut fiber was least efficient to develop roots and aerial parts of plants. Better results were showed by substratum only with carbonized rice husk + husk pine on culture of Cattleya intermedia X Hadrolaelia purpurata.O presente trabalho teve como objetivo avaliar a eficiência de diferentes substratos de origem vegetal no cultivo de espécies de orquídeas. Plântulas de Cattleya intermedia X Hadrolaelia purpurata, provenientes de cultivo in vitro, aclimatizadas e com altura média de 5 cm, foram plantadas nos substratos: 1-Xaxim desfibrado (testemunha; 2-Casca de arroz carbonizada; 3-Fibra de coco; 4-Casca de pínus; 5-Casca de pínus + Fibra de coco (1:1; 6-Casca de arroz + Fibra de coco (1:1; 7-Casca de arroz carbonizada + Casca de pínus (1:1; 8-Casca de pínus + Casca de arroz carbonizada + Fibra de coco (1:1:1. O delineamento experimental foi o inteiramente casualizado com oito tratamentos e dez repeti

Evaluation of anti-microbial activity of spore powder of Ganoderma lucidum on clinical isolates of Prevotella intermedia: A pilot study

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Ranganath N Nayak

2015-01-01

Full Text Available Aim: This study aimed at evaluating the anti-microbial activity of spore powder of Ganoderma lucidum on Prevotella intermedia isolated from subgingival plaque from chronic periodontitis patients. Settings and Design: Written informed consent was obtained from each subject enrolled in the study. The Institutional Ethics Committee granted the ethical clearance for the study. Materials and Methods: This study included 20 patients diagnosed with chronic periodontitis. Pooled subgingival plaque samples were collected using sterile curettes from the deepest sites of periodontal pockets. The collected samples were then transported in 1 mL of reduced transport fluid. The organisms were cultured and confirmed. These organisms were then used for minimum inhibitory concentration (MIC procedure. Statistical Analysis: Mean of the MIC value obtained was calculated. Results: Thirteen out of the 20 clinical samples were tested that showed sensitivity at various concentrations. Five samples showed sensitivity at all concentrations. Twelve samples showed sensitivity at 8 mcg/ml. Eleven samples showed sensitivity at 4 mcg/ml, 8 samples showed sensitivity at 2 mcg/ml, and 5 samples showed sensitivity even at 1 mcg/ml. Mean MIC value of G. lucidum spore powder for P. intermedia obtained was 3.62 mcg/ml. Conclusion: G. lucidum with its multipotential bioactivity could be used as an anti-microbial, in conjunction with conventional therapy in periodontal disease.

Gibberella intermedia the pathogen of St. John's Wort, coneflower and marshmallow in Serbia

OpenAIRE

Pavlović Snežana Đ.; Stojšin Vera B.; Stojanović Saša D.; Starović Mira S.; Bagi Ferenc F.; Budakov Dragana B.

2009-01-01

Gibberella intermedia (Kuhlmann) Samuels et al. (anamorf: Fusarium proliferatum /Matsushima/ Nirenberg) was isolated from seeds of St. John's wort, marshmallow, and coneflower, as well as from roots and stalks of marshmallow and roots of coneflower. These plants had symptoms of leaf chlorosis, malformation, withering and plant dwarfing and were collected from several localities in Serbia during five-year investigations of mycopopulations of the mentioned plants. The morphological characterist...

First evidence of SGPL1 expression in the cell membrane silencing the extracellular S1P siren in mammary epithelial cells.

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Engel, Nadja; Adamus, Anna; Frank, Marcus; Kraft, Karin; Kühn, Juliane; Müller, Petra; Nebe, Barbara; Kasten, Annika; Seitz, Guido

2018-01-01

The bioactive lipid sphingosine-1-phosphate (S1P) is a main regulator of cell survival, proliferation, motility, and platelet aggregation, and it is essential for angiogenesis and lymphocyte trafficking. In that S1P acts as a second messenger intra- and extracellularly, it might promote cancer progression. The main cause is found in the high S1P concentration in the blood, which encourage cancer cells to migrate through the endothelial barrier into the blood vessels. The irreversible degradation of S1P is solely caused by the sphingosine-1-phosphate lyase (SGPL1). SGPL1 overexpression reduces cancer cell migration and therefore silences the endogenous S1P siren, which promotes cancer cell attraction-the main reason for metastasis. Since our previous metabolomics studies revealed an increased SGPL1 activity in association with successful breast cancer cell treatment in vitro, we further investigated expression and localization of SGPL1. Expression analyses confirmed a very low SGPL1 expression in all breast cancer samples, regardless of their subtype. Additionally, we were able to prove a novel SGPL expression in the cytoplasm membrane of non-tumorigenic breast cells by fusing three independent methods. The general SGPL1 downregulation and the loss of the plasma membrane expression resulted in S1P dependent stimulation of migration in the breast cancer cell lines MCF-7 and BT-20. Not only S1P stimulated migration could be repressed by overexpressing the natural SGPL1 variant not but also more general migratory activity was significantly reduced. Here, for the first time, we report on the SGPL1 plasma membrane location in human, non-malignant breast epithelial cell lines silencing the extracellular S1P siren in vitro, and thereby regulating pivotal cellular functions. Loss of this plasma membrane distribution as well as low SGPL1 expression levels could be a potential prognostic marker and a viable target for therapy. Therefore, the precise role of SGPL1 for cancer

First evidence of SGPL1 expression in the cell membrane silencing the extracellular S1P siren in mammary epithelial cells.

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Nadja Engel

Full Text Available The bioactive lipid sphingosine-1-phosphate (S1P is a main regulator of cell survival, proliferation, motility, and platelet aggregation, and it is essential for angiogenesis and lymphocyte trafficking. In that S1P acts as a second messenger intra- and extracellularly, it might promote cancer progression. The main cause is found in the high S1P concentration in the blood, which encourage cancer cells to migrate through the endothelial barrier into the blood vessels. The irreversible degradation of S1P is solely caused by the sphingosine-1-phosphate lyase (SGPL1. SGPL1 overexpression reduces cancer cell migration and therefore silences the endogenous S1P siren, which promotes cancer cell attraction-the main reason for metastasis. Since our previous metabolomics studies revealed an increased SGPL1 activity in association with successful breast cancer cell treatment in vitro, we further investigated expression and localization of SGPL1. Expression analyses confirmed a very low SGPL1 expression in all breast cancer samples, regardless of their subtype. Additionally, we were able to prove a novel SGPL expression in the cytoplasm membrane of non-tumorigenic breast cells by fusing three independent methods. The general SGPL1 downregulation and the loss of the plasma membrane expression resulted in S1P dependent stimulation of migration in the breast cancer cell lines MCF-7 and BT-20. Not only S1P stimulated migration could be repressed by overexpressing the natural SGPL1 variant not but also more general migratory activity was significantly reduced. Here, for the first time, we report on the SGPL1 plasma membrane location in human, non-malignant breast epithelial cell lines silencing the extracellular S1P siren in vitro, and thereby regulating pivotal cellular functions. Loss of this plasma membrane distribution as well as low SGPL1 expression levels could be a potential prognostic marker and a viable target for therapy. Therefore, the precise role of

Identification of the WRKY gene family and functional analysis of two genes in Caragana intermedia.

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Wan, Yongqing; Mao, Mingzhu; Wan, Dongli; Yang, Qi; Yang, Feiyun; Mandlaa; Li, Guojing; Wang, Ruigang

2018-02-09

WRKY transcription factors, one of the largest families of transcriptional regulators in plants, play important roles in plant development and various stress responses. The WRKYs of Caragana intermedia are still not well characterized, although many WRKYs have been identified in various plant species. We identified 53 CiWRKY genes from C. intermedia transcriptome data, 28 of which exhibited complete open reading frames (ORFs). These CiWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and zinc finger motifs. Conserved domain analysis showed that the CiWRKY proteins contain a highly conserved WRKYGQK motif and two variant motifs (WRKYGKK and WKKYEEK). The subcellular localization of CiWRKY26 and CiWRKY28-1 indicated that these two proteins localized exclusively to nuclei, supporting their role as transcription factors. The expression patterns of the 28 CiWRKYs with complete ORFs were examined through quantitative real-time PCR (qRT-PCR) in various tissues and under different abiotic stresses (drought, cold, salt, high-pH and abscisic acid (ABA)). The results showed that each CiWRKY responded to at least one stress treatment. Furthermore, overexpression of CiWRKY75-1 and CiWRKY40-4 in Arabidopsis thaliana suppressed the drought stress tolerance of the plants and delayed leaf senescence, respectively. Fifty-three CiWRKY genes from the C. intermedia transcriptome were identified and divided into three groups via phylogenetic analysis. The expression patterns of the 28 CiWRKYs under different abiotic stresses suggested that each CiWRKY responded to at least one stress treatment. Overexpression of CiWRKY75-1 and CiWRKY40-4 suppressed the drought stress tolerance of Arabidopsis and delayed leaf senescence, respectively. These results provide a basis for the molecular mechanism through which CiWRKYs mediate stress tolerance.

Partial Splenectomy in the treatment of an adult with β thalassemia intermedia: A case report.

Science.gov (United States)

Correia, João Guardado; Moreira, Nídia; Costa Almeida, Carlos Eduardo; Reis, Luís Simões

2017-01-01

Thalassemia is a common disease which treatment is often based on splenectomy. The risks associated with total splenectomy stimulated partial splenectomy as a potentially alternative therapy. A 45 year-old female patient with long term follow-up for β thalassemia intermedia started to develop signs of hypersplenism and iron overload. A partial splenectomy was performed and was observed a marked hematologic improvement while preserving the desired splenic function. Partial splenectomy proved to provide a persistent decrease in hemolytic rate while preserving the integrity of splenic phagocytic function, presenting itself as an effective alternative to total splenectomy. After being subjected to partial splenectomy, our patient experienced a sustained control of hemolysis and showed no signs of hypersplenism or iron overload. No splenic regrowth or infectious complications were observed. The major drawbacks of partial splenectomy are the increased risk of intra- and postoperative bleeding, splenic remnant torsion and splenic regrowth. Partial splenectomy is an alternative to total splenectomy for the treatment of adult β Thalassemia intermedia patients avoiding the risks associated with total splenectomy.

Detection of Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia, and Prevotella nigrescens in chronic endodontic infection.

Science.gov (United States)

Tomazinho, Luiz Fernando; Avila-Campos, Mario J

2007-02-01

Black-pigmented anaerobic rods such as Prevotella spp. and Porphyromonas spp. are involved in the etiology and perpetuation of endodontic infections. The aim of this study was to evaluate the prevalence of these species in chronic endodontic infections by using culture and polymerase chain reaction (PCR) techniques. Samples of 100 patients with root canals displaying chronic endodontic infections were obtained by sterilized paper points. Bacterial identification was performed by using culture and PCR techniques. By culture, in 33% of the samples, P. intermedia-P. nigrescens (75.8%), P. gingivalis (27.3%), and P. endodontalis (9.1%) were identified, and by PCR 60% of the samples harbored P. nigrescens (43.3%), P. gingivalis (43.3%), P. intermedia (31.7%), and P. endodontalis (23.3%). The presence of these black-pigmented anaerobic rods alone or in association in chronic endodontic infections seems to be frequent. PCR is a very sensitive technique for detecting DNA from bacterial cells. Culturing is only able to reveal living bacteria and is less sensitive for the identification of low numbers of bacterial cells.

Characterization of pars intermedia connections in amphibians by biocytin tract tracing and immunofluorescence aided by confocal microscopy

NARCIS (Netherlands)

Jansen, K; Fabro, C; Artero, C; Feuilloley, M; Vaudry, H; Fasolo, A; Franzoni, MF

Biocytin, recently introduced in neuroanatomical studies, was used as a retrograde tract tracer in combination with immunofluorescence in order to analyse the neurochemical characters of some central neuronal projections to the pars intermedia in two amphibian species, the anuran Rana esculenta and

Noninvasive analysis of skin iron and zinc levels in beta-thalassemia major and intermedia

International Nuclear Information System (INIS)

Gorodetsky, R.; Goldfarb, A.; Dagan, I.; Rachmilewitz, E.A.

1985-01-01

Diagnostic x-ray spectrometry, a method based on x-ray fluorescence analysis, was used for noninvasive determination of iron and zinc in two distinct skin areas, representing predominantly dermal and epidermal tissues, in 56 patients with beta-thalassemia major and intermedia. The mean iron levels in the skin of patients with beta-thalassemia major and intermedia were elevated by greater than 200% and greater than 50%, respectively, compared with control values. The zinc levels of both skin areas examined were within the normal range. The data indicate that the rate and number of blood transfusions, which correlated well with serum ferritin levels (r . 0.8), are not the only factors that determine the amount of iron deposition in the skin (r less than 0.6). Other sources of iron intake contribute to the total iron load in the tissues, particularly in patients who are not given multiple transfusions. The noninvasive quantitation of skin levels may reflect the extent of iron deposition in major parenchymal organs. Repeated DXS examinations of the skin could monitor the clearance of iron from the tissues of patients with iron overload in the course of therapy with chelating agents

The Effects of Gravitation on the Inter-Media Agenda-Setting Central Process: The Case of the Murder of Hrant Dink

Directory of Open Access Journals (Sweden)

Cem YAŞIN

2014-02-01

Full Text Available While the first level agenda setting researches focus on the transfer of issue salience from the media to public agenda, second level agenda setting researches interest in the attributes emphasized in the news and their affect on the public agenda. Some of these researches tends to analysis the media agenda. Influences of the news media on each other are studied by the inter-media agenda setting researches at at both the first and second levels. The same researches examine also the effects of different types of media on each other. However there is the problem of lack of a systematic theoretical model. This is caused by the differentiation in the aims of researchers and in their research objects. The other problem in the inter-media agenda setting researches is that there is no research on the agenda setting effects of the newspapers which have different ideological and political identities. This research aims to scrutinize the inter-media agenda-setting effects among various newspapers that have got different points of view. The research is designed to test the central gravitation effects of the mainstream news papers. Here the Murder of Hrant Dink is selected as a case study.

[Effects of cutting and reseeding on the ground-dwelling arthropod community in Caragana intermedia forest in desert steppe].

Science.gov (United States)

Liu, Ren-Tao; Chai, Yong-Qing; Yang, Xin-Guo; Song, Nai-Ping; Wang, Xin-Yun; Wang, Lei

2013-01-01

Taking a 25-year-old Caragana intermedia forest in desert steppe as test object, an investigation was conducted on the ground-dwelling arthropod community in cutting and no-cutting stands with and without reseeding, aimed to understand the effects of cutting, reseeding and their interaction on the individual number and group richness of ground-dwelling arthropod in C. intermedia forest. There were significantly lower number and richness of ground-dwelling arthropod in the open spaces than under the shrubs in the no-cutting and no-reseeding stands. Cutting, reseeding and both of them could significantly increase the number and richness of ground-dwelling arthropod in the open spaces, but not under the shrubs, compared with no cutting or reseeding. Consequently, there were no significant differences in the distribution of ground-dwelling arthropod in the open spaces and under the shrubs in the cutting, reseeding, or cutting and reseeding stands. Further, there was a similar buffer effect between cutting and reseeding on the ground-dwelling arthropod. No significant differences were observed in the ground-dwelling arthropod distribution, between cutting stand and reseeding stand, between cutting stand and cutting and reseeding stand, and between reseeding stand and cutting and reseeding stand. It was suggested that cutting, reseeding, or both of them could significantly improve the ground-dwelling arthropod diversity especially in the open spaces, being beneficial for the restoration of degraded grassland ecosystem and the rational management on artificial C. intermedia forest in desert steppe.

Nuevos sistemas de frecuencia intermedia para el IAR

Science.gov (United States)

Olalde, J. C.; Perilli, D.; Larrarte, J. J.

Se presenta el diagrama en bloques de los nuevos sistemas de Frecuencia Intermedia para los dos radiómetros instalados en el IAR. Entre las características más importantes del sistema podemos mencionar la posibilidad de conectar cualquiera de las dos antenas a los ``backend" disponibles: analizador espectral de alta resolución (META II) de 0,05 Hz, autocorrelador de 1008 canales y contínuo. Se incorporan al sistema nuevos sintetizadores de frecuencia implementados con PLL y la moderna técnica de síntesis digital directa. Por último, el conjunto del sistema es susceptible de ser configurado por las computadoras de adquisición de datos, supervisadas por otra, que entrega el estado de funcionamiento actual y evita la selección de configuraciones incorrectas por parte del usuario.

Suppression of linalool acetate production in Lavandula x intermedia.

Science.gov (United States)

Desautels, Amy; Biswas, Kamal; Lane, Alexander; Boeckelmann, Astrid; Mahmoud, Soheil S

2009-11-01

Linalool acetate, one of the major constituent of several essential oils, is heat-labile and decomposes upon exposure to the high injector temperature during gas chromatography. Here we report the development of an improved method for detection of this compound by gas chromatography mass spectrometry (GCMS) using cold on-column injection of the sample. By using this sensitive method, it has been demonstrated that a lavandin (L. x intermedia) mutant accumulates trace quantities of linalool acetate and camphor and higher amounts of cineole and borneol compared to its parent. This plant, which very likely carries a point mutation in one or more of the genes involved in essential oil production, provides a unique tool for investigating regulation of essential oil biogenesis in plants.

Los límites de la ciudad intermedia : conectividad e innovación

OpenAIRE

Basurto Alvarez, Rodolfo

2014-01-01

Este trabajo vincula conectividad e innovación para fijar los alcances de la segunda modernidad de una ciudad intermedia (Hermosillo, Sonora, México), estableciendo sus restricciones para ascender como nodo de comunicación sur-norte de la ciudad global. Se evalúa la diversidad incipiente del ecosistema urbano mediante el referente hipotético de la existencia de flujos comunicacionales de una sola dirección (norte-sur) en el funcionamiento de las empresas multinacionales. ...

Gibberella intermedia the pathogen of St. John's Wort, coneflower and marshmallow in Serbia

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Pavlović Snežana Đ.

2009-01-01

Full Text Available Gibberella intermedia (Kuhlmann Samuels et al. (anamorf: Fusarium proliferatum /Matsushima/ Nirenberg was isolated from seeds of St. John's wort, marshmallow, and coneflower, as well as from roots and stalks of marshmallow and roots of coneflower. These plants had symptoms of leaf chlorosis, malformation, withering and plant dwarfing and were collected from several localities in Serbia during five-year investigations of mycopopulations of the mentioned plants. The morphological characteristics of the pathogen were described.

Identification of microRNAs in Caragana intermedia by high-throughput sequencing and expression analysis of 12 microRNAs and their targets under salt stress.

Science.gov (United States)

Zhu, Jianfeng; Li, Wanfeng; Yang, Wenhua; Qi, Liwang; Han, Suying

2013-09-01

142 miRNAs were identified and 38 miRNA targets were predicted, 4 of which were validated, in C. intermedia . The expression of 12 miRNAs in salt-stressed leaves was assessed by qRT-PCR. MicroRNAs (miRNAs) are endogenous small RNAs that play important roles in various biological and metabolic processes in plants. Caragana intermedia is an important ecological and economic tree species prominent in the desert environment of west and northwest China. To date, no investigation into C. intermedia miRNAs has been reported. In this study, high-throughput sequencing of small RNAs and analysis of transcriptome data were performed to identify both conserved and novel miRNAs, and also their target mRNA genes in C. intermedia. Based on sequence similarity and hairpin structure prediction, 132 putative conserved miRNAs (12 of which were confirmed to form hairpin precursors) belonging to 31 known miRNA families were identified. Ten novel miRNAs (including the miRNA* sequences of three novel miRNAs) were also discovered. Furthermore, 36 potential target genes of 17 known miRNA families and 2 potential target genes of 1 novel miRNA were predicted; 4 of these were validated by 5' RACE. The expression of 12 miRNAs was validated in different tissues, and these and five target mRNAs were assessed by qRT-PCR after salt treatment. The expression levels of seven miRNAs (cin-miR157a, cin-miR159a, cin-miR165a, cin-miR167b, cin-miR172b, cin-miR390a and cin-miR396a) were upregulated, while cin-miR398a expression was downregulated after salt treatment. The targets of cin-miR157a, cin-miR165a, cin-miR172b and cin-miR396a were downregulated and showed an approximately negative correlation with their corresponding miRNAs under salt treatment. These results would help further understanding of miRNA regulation in response to abiotic stress in C. intermedia.

Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens in endodontic lesions detected by culture and by PCR.

Science.gov (United States)

Gomes, B P F A; Jacinto, R C; Pinheiro, E T; Sousa, E L R; Zaia, A A; Ferraz, C C R; Souza-Filho, F J

2005-08-01

he aim of this study was to investigate the presence of four black-pigmented bacteria, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens, in endodontic infections by culture and polymerase chain reaction (PCR) analyses. Microbial samples were obtained from 50 teeth with untreated necrotic pulps (primary infection) and from 50 teeth with failing endodontic treatment (secondary infection). Microbiological strict anaerobic techniques were used for serial dilution, plating, incubation, and identification. For PCR detection, the samples were analyzed using species-specific primers of 16S rDNA and the downstream intergenic spacer region. Culture and PCR detected the test species in 13/100 and 50/100 of the study teeth, respectively. The organisms were cultured from 11/50 (22%) of primarily infected root canal samples and from 2/50 (4%) of secondary root canal samples. PCR detection identified the target species in 32/50 (64%) and 18/50 (36%) of primary and secondary infections, respectively. P. gingivalis was rarely isolated by culture methods (1%), but was the most frequently identified test species by PCR (38%). Similarly, P. endodontalis was not recovered by culture from any tooth studied, but was detected by PCR in 25% of the sampled teeth. PCR-based identification also showed higher detection rates of P. intermedia (33%) and P. nigrescens (22%) than culture (13%). In conclusion, P. gingivalis, P. endodontalis, P. intermedia, and P. nigrescens were identified more frequently in teeth with necrotic pulp than in teeth with failing endodontic treatment. Also, a higher frequency of black-pigmented species was detected by PCR than by culture.

Polarization of the interference field during reflection of electromagnetic waves from an intermedia boundary

Science.gov (United States)

Bulakhov, M. G.; Buyanov, Yu. I.; Yakubov, V. P.

1996-10-01

It has been shown that a full vector measurement of the total field allows one to uniquely distinguish the incident and reflected waves at each observation point without the use of a spatial difference based on an analysis of the polarization structure of the interference pattern which arises during reflection of electromagnetic waves from an intermedia boundary. We have investigated the stability of these procedures with respect to measurement noise by means of numerical modeling.

Detection and genetic characterization of β-lactamases in Prevotella intermedia and Prevotella nigrescens isolated from oral cavity infections and peritonsillar abscesses.

Science.gov (United States)

Fernández-Canigia, Liliana; Cejas, Daniela; Gutkind, Gabriel; Radice, Marcela

2015-06-01

A prospective analysis on β-lactam resistance mechanisms and β-lactamase prevalence was conducted on Prevotella intermedia and Prevotella nigrescens recovered from patients with chronic periodontitis and peritonsillar abscesses. Both phenotypic and genotypic methods were performed to characterize the β-lactamases, their coding genes and their genetic contexts. Overall, β-lactamase production was observed in 64% (16/25) P. intermedia and 23.8% (5/21) P. nigrescens (p intermedia (8/16) than in P. nigrescens (2/16) recovered from chronic periodontitis, almost all isolates from peritonsillar abscesses were producers (8/9 and 3/3, respectively). cfxA, but not cepA and cblA, was detected in those isolates, which were previously categorized as β-lactamase producers. CfxA producing isolates displayed higher β-lactam MICs than non-producers in both species. The most frequent allele was cfxA2, followed by cfxA3 and a new allelic variant named cfxA6. The analysis of the downstream flanking region in the three cfxA variants revealed the association with mobA of Tn4555, suggesting their localization in a mobilizable element. β-lactam resistance and cfxA carriage prevalence seems to be not only related to the bacterial species but also to the infection site. Copyright © 2015 Elsevier Ltd. All rights reserved.

Evaluation of antioxidant potential of Lavandula x intermedia Emeric ex Loisel. 'Budrovka': a comparative study with L. angustifolia Mill.

Science.gov (United States)

Blazeković, Biljana; Vladimir-Knezević, Sanda; Brantner, Adelheid; Stefan, Maja Bival

2010-08-30

A Croatian indigenous cultivar of lavandin, Lavandula x intermedia 'Budrovka' (Lamiaceae) was studied for the phenolic acids, flavonoids, anthocyanins, procyanidins and total tannins, as well as total polyphenols content, in the flower, inflorescence stalk and leaf ethanolic extracts. Antioxidant potentials on these plant part extracts were assessed by the DPPH free radical scavenging activity, iron chelating activity, reducing power, lipid peroxidation inhibition properties and total antioxidant capacity assays. All results were compared with those of Lavandula angustifolia, the only member of the Lavandula genus officially used in modern phytotherapy. Based on the results of our parallel study, we may suggest that Lavandula x intermedia 'Budrovka' is as potent an antioxidant as Lavandula angustifolia and the antioxidant activity of the Lavandula extracts is mainly due to the presence of rosmarinic acid. A good correlation was found between the polyphenol contents and antioxidant activities of the extracts.

Evaluation of Antioxidant Potential of Lavandula x intermedia Emeric ex Loisel. 'Budrovka': A Comparative Study with L. angustifolia Mill.

Directory of Open Access Journals (Sweden)

Maja Bival Štefan

2010-08-01

Full Text Available A Croatian indigenous cultivar of lavandin, Lavandula x intermedia 'Budrovka' (Lamiaceae was studied for the phenolic acids, flavonoids, anthocyanins, procyanidins and total tannins, as well as total polyphenols content, in the flower, inflorescence stalk and leaf ethanolic extracts. Antioxidant potentials on these plant part extracts were assessed by the DPPH free radical scavenging activity, iron chelating activity, reducing power, lipid peroxidation inhibition properties and total antioxidant capacity assays. All results were compared with those of Lavandula angustifolia, the only member of the Lavandula genus officially used in modern phytotherapy. Based on the results of our parallel study, we may suggest that Lavandula x intermedia 'Budrovka' is as potent an antioxidant as Lavandula angustifolia and the antioxidant activity of the Lavandula extracts is mainly due to the presence of rosmarinic acid. A good correlation was found between the polyphenol contents and antioxidant activities of the extracts

Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom

International Nuclear Information System (INIS)

Ullah, Anwar; Giuseppe, Priscila Oliveira de; Murakami, Mario Tyago; Trevisan-Silva, Dilza; Wille, Ana Carolina Martins; Chaves-Moreira, Daniele; Gremski, Luiza Helena; Silveira, Rafael Bertoni da; Sennf-Ribeiro, Andrea; Chaim, Olga Meiri; Veiga, Silvio Sanches; Arni, Raghuvir Krishnaswamy

2011-01-01

Wild-type and H12A-mutant class II phospholipase D from L. intermedia venom were crystallized; the crystals diffracted to maximum resolutions of 1.95 and 1.60 Å, respectively. Phospholipases D are the major dermonecrotic component of Loxosceles venom and catalyze the hydrolysis of phospholipids, resulting in the formation of lipid mediators such as ceramide-1-phosphate and lysophosphatidic acid which can induce pathological and biological responses. Phospholipases D can be classified into two classes depending on their catalytic efficiency and the presence of an additional disulfide bridge. In this work, both wild-type and H12A-mutant forms of the class II phospholipase D from L. intermedia venom were crystallized. Wild-type and H12A-mutant crystals were grown under very similar conditions using PEG 200 as a precipitant and belonged to space group P12 1 1, with unit-cell parameters a = 50.1, b = 49.5, c = 56.5 Å, β = 105.9°. Wild-type and H12A-mutant crystals diffracted to maximum resolutions of 1.95 and 1.60 Å, respectively

Intermediação no futebol profissional : regime e âmbitos contratuais

OpenAIRE

Marques, Fernanda Duarte de Barros, 1973-

2018-01-01

Dissertação de mestrado em Direito, Universidade Lusíada de Lisboa, 2017 Exame público realizado em 23 de Janeiro de 2018 A presente dissertação de mestrado tem por objecto nuclear a análise do regime jurídico da actividade do empresário de futebol e do respectivo contrato que este firma com os jogadores de futebol ou com os clubes/sociedades desportivas vocacionado para a sua intermediação na celebração de contratos desportivos. A versátil, elástica e complexa actividade do empresário ...

Evolución cenozoica de la Depresión Intermedia (Cuenca-Guadalajara)

OpenAIRE

Torres, T.; Zapata, J. L.

1987-01-01

En este trabajo se analiza la evolución Cenozoica de la Depresión Intermedia, habiéndose determinado la existencia de dos ciclos paleógenos y cuatro neógenos. En todos ellos la sedimentación es continental y de carácter fluvial y lacustre-palustre. Si se exceptúa el Primer Ciclo Paleógeno, que tuvo su área fuente en una zona al sur de la cuenca en la que predominaban rocas paleozoicas, los cinco restantes las tuvieron en el Sistema Ibérico, donde predominan rocas mesozoicas

Evolución cenozoica de la Depresión Intermedia (Cuenca-Guadalajara)

OpenAIRE

Torres Pérez-Hidalgo, Trinidad José

1987-01-01

En este trabajo se analiza la evolución Cenozoica de la Depresión Intermedia, habiéndose determinado la existencia de dos ciclos paleógenos y cuatro neógenos. En todos ellos la sedimentación es continental y de carácter fluvial y lacustre-palustre. Si se exceptúa el Primer Ciclo Paleógeno, que tuvo su área fuente en una zona al sur de la cuenca en la que predominaban rocas paleozoicas, los cinco restantes las tuvieron en el Sistema Ibérico, donde predominan rocas mesozoicas....

Genistein suppresses Prevotella intermedia lipopolysaccharide-induced inflammatory response in macrophages and attenuates alveolar bone loss in ligature-induced periodontitis.

Science.gov (United States)

Choi, Eun-Young; Bae, Seung Han; Ha, Min Hee; Choe, So-Hui; Hyeon, Jin-Yi; Choi, Jeom-Il; Choi, In Soon; Kim, Sung-Jo

2016-02-01

Genistein is a major isoflavone subclass of flavonoids found in soybean and a potent tyrosine kinase inhibitor. The present study aimed to assess the effect of genistein on the production of proinflammatory mediators in murine macrophages stimulated with lipopolysaccharide (LPS) isolated from Prevotella intermedia, a pathogen associated with different forms of periodontal disease, and to evaluate its possible influence on alveolar bone loss in ligature-induced periodontitis using micro-computed tomography (micro-CT) analysis as well. LPS was isolated from P. intermedia ATCC 25611 by using the standard hot phenol-water method. Culture supernatants were analyzed for nitric oxide (NO) and interleukin-6 (IL-6). Inducible NO synthase (iNOS) protein expression was evaluated by immunoblot analysis. Real-time PCR was carried out to measure iNOS and IL-6 mRNA expression. In addition, effect of genistein on alveolar bone loss was evaluated in a rat model of experimental periodontitis using micro-CT analysis. Genistein significantly attenuated P. intermedia LPS-induced production of iNOS-derived NO and IL-6 with attendant decrease in their mRNA expression in RAW264.7 cells. In addition, when genistein was administered to rats, decreases in alveolar bone height and bone volume fraction induced by ligature placement were significantly inhibited. Genistein administration also prevented ligature-induced alterations in the microstructural parameters of trabecular bone, including trabecular thickness, trabecular separation, bone mineral density and structure model index. While additional studies are required, we suggest that genistein could be utilized for the therapy of human periodontitis in the future. Copyright © 2015 Elsevier Ltd. All rights reserved.

La organización de los actores en el desarrollo territorial. El tren de alta velocidad en la ciudad intermedia

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Jaume Feliu Torrent

2007-01-01

Full Text Available Si queremos evaluar el grado de desarrollo local que aporta el Tren de Alta Velocidad (TAV en una ciudad es primordial observar la capacidad de los actores del territorio de organizarse para aprovechar sus recursos endógenos. Mediante la elaboración de una nueva metodología, se propone el estudio del proceso de desarrollo de tres ciudades intermedias con TAV, Lérida (España, Aviñón (Francia y Novara (Italia. En las conclusiones se ponen de manifiesto ciertos problemas propios de la ciudad intermedia en la ejecución de su proyecto local, básicamente la existencia de una relación multiescalar conflictiva y de una débil red de actores locales. La fragilidad de este contexto urbano es debida a la falta de correctos procesos de gobernanza interna y externa.

Network of siren, public address and display system to preparedness and response for nuclear emergencies

International Nuclear Information System (INIS)

Joshi, G.H.; Padmanabhan, N.; Raman, N.; Pradeepkumar, K.S.; Sharma, D.N.; Abani, M.C.

2003-01-01

For an effective emergency response and implementation of counter measures, communication during a nuclear emergency is a very important aspect. The declaration of a nuclear emergency must be immediately conveyed to all those working in the plant and around the nuclear site. Besides this, the nature of emergency also needs to be conveyed unambiguously along with corresponding counter measures, such as stay in, evacuation or all clear signal for the relevant plants. This requirement has necessitated the need for a networked signaling system. Based on this requirement, a microcontroller based signaling and a telephone/wireless based communication and display system has been designed at Bhabha Atomic Research Centre. It is proposed to be used as a part of emergency preparedness and response programme at the nuclear facility sites. As per the design made for Bhabha Atomic Research Centre, Trombay site, each plant or area in the site is identified by a unique identification code. The main Site Emergency Control Centre/Emergency Response Centre at Mod. Labs. selectively calls the various plants and declares the nature of emergency to be followed In that plant/area through different siren signals along with display and announcement of instructions. This paper describes the details of the system that is designed for Bhabha Atomic Research Centre, Trombay site and proposed for other nuclear power plant sites. (author)

Spatial Heterogeneity of Soil Nutrients after the Establishment of Caragana intermedia Plantation on Sand Dunes in Alpine Sandy Land of the Tibet Plateau.

Science.gov (United States)

Li, Qingxue; Jia, Zhiqing; Zhu, Yajuan; Wang, Yongsheng; Li, Hong; Yang, Defu; Zhao, Xuebin

2015-01-01

The Gonghe Basin region of the Tibet Plateau is severely affected by desertification. Compared with other desertified land, the main features of this region is windy, cold and short growing season, resulting in relatively difficult for vegetation restoration. In this harsh environment, identification the spatial distribution of soil nutrients and analysis its impact factors after vegetation establishment will be helpful for understanding the ecological relationship between soil and environment. Therefore, in this study, the 12-year-old C. intermedia plantation on sand dunes was selected as the experimental site. Soil samples were collected under and between shrubs on the windward slopes, dune tops and leeward slopes with different soil depth. Then analyzed soil organic matter (SOM), total nitrogen (TN), total phosphorus (TP), total potassium (TK), available nitrogen (AN), available phosphorus (AP) and available potassium (AK). The results showed that the spatial heterogeneity of soil nutrients was existed in C. intermedia plantation on sand dunes. (1) Depth was the most important impact factor, soil nutrients were decreased with greater soil depth. One of the possible reasons is that windblown fine materials and litters were accumulated on surface soil, when they were decomposed, more nutrients were aggregated on surface soil. (2) Topography also affected the distribution of soil nutrients, more soil nutrients distributed on windward slopes. The herbaceous coverage were higher and C. intermedia ground diameter were larger on windward slopes, both of them probably related to the high soil nutrients level for windward slopes. (3) Soil "fertile islands" were formed, and the "fertile islands" were more marked on lower soil nutrients level topography positions, while it decreased towards higher soil nutrients level topography positions. The enrichment ratio (E) for TN and AN were higher than other nutrients, most likely because C. intermedia is a leguminous shrub.

CITYMARKETING EN CIUDADES INTERMEDIAS: CASO PALMIRA

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Miguel Ezequiel Badillo Mendoza

2010-01-01

Full Text Available En este artículo se presentan los resultados de la investigación Citymarketing en ciudades intermedias: caso Palmira, específicamente en su etapa de diseño. Este proyecto surgió de la necesidad de generar acciones académicas y metodológicas para desarrollar un mecanismo que permitiera el diseño de un instrumento de gestión y promoción de la ciudad. La población de estudio se integró con habitantes de la ciudad, visitantes y dirigentes de diversos sectores del departamento del Valle del Cauca. La metodología empleada se basó en un estudio documental y el uso de instrumentos cualitativos, como cartografías urbanas, estudio de imaginarios y mediciones cuantitativas de comunicación publicitaria. Para su realización se estructuró en dos fases: La primera donde se estableció un eje conceptual para la implementación de un Plan de citymarketing en Palmira y una fase 2: donde se desarrolló la sensibilización, la caracterización de la ciudad, los lineamientos estratégicos, los parámetros para la construcción de la marca y el diseño del Plan de Comunicaciones.

Full structure and insight into the gene cluster of the O-specific polysaccharide of Yersinia intermedia H9-36/83 (O:17).

Science.gov (United States)

Sizova, Olga V; Shashkov, Alexander S; Kondakova, Anna N; Knirel, Yuriy A; Shaikhutdinova, Rima Z; Ivanov, Sergei A; Kislichkina, Angelina A; Kadnikova, Lidia A; Bogun, Aleksandr G; Dentovskaya, Svetlana V

2018-05-02

Lipopolysaccharide was isolated from bacteria Yersinia intermedia H9-36/83 (O:17) and degraded with mild acid to give an O-specific polysaccharide, which was isolated by GPC on Sephadex G-50 and studied by sugar analysis and 1D and 2D NMR spectroscopy. The polysaccharide was found to contain 3-deoxy-3-[(R)-3-hydroxybutanoylamino]-d-fucose (d-Fuc3NR3Hb) and the following structure of the heptasaccharide repeating unit was established: The structure established is consistent with the gene content of the O-antigen gene cluster. The O-polysaccharide structure and gene cluster of Y. intermedia are related to those of Hafnia alvei 1211 and Escherichia coli O:103. Copyright © 2018 Elsevier Ltd. All rights reserved.

Nanopore Long-Read Guided Complete Genome Assembly of Hydrogenophaga intermedia, and Genomic Insights into 4-Aminobenzenesulfonate, p-Aminobenzoic Acid and Hydrogen Metabolism in the Genus Hydrogenophaga.

Science.gov (United States)

Gan, Han M; Lee, Yin P; Austin, Christopher M

2017-01-01

We improved upon the previously reported draft genome of Hydrogenophaga intermedia strain PBC, a 4-aminobenzenesulfonate-degrading bacterium, by supplementing the assembly with Nanopore long reads which enabled the reconstruction of the genome as a single contig. From the complete genome, major genes responsible for the catabolism of 4-aminobenzenesulfonate in strain PBC are clustered in two distinct genomic regions. Although the catabolic genes for 4-sulfocatechol, the deaminated product of 4-aminobenzenesulfonate, are only found in H. intermedia , the sad operon responsible for the first deamination step of 4-aminobenzenesulfonate is conserved in various Hydrogenophaga strains. The absence of pabB gene in the complete genome of H. intermedia PBC is consistent with its p -aminobenzoic acid (pABA) auxotrophy but surprisingly comparative genomics analysis of 14 Hydrogenophaga genomes indicate that pABA auxotrophy is not an uncommon feature among members of this genus. Of even more interest, several Hydrogenophaga strains do not possess the genomic potential for hydrogen oxidation, calling for a revision to the taxonomic description of Hydrogenophaga as "hydrogen eating bacteria."

In Vitro Antibacterial and Antifungal Activity of Lavandula x intermedia Emeric ex Loisel. ‘Budrovka’

OpenAIRE

Blazekovic, Biljana; Stanic, Gordana; Pepeljnjak, Stjepan; Vladimir-Knezevic, Sanda

2011-01-01

This study aimed to evaluate the in vitro antibacterial and antifungal activities of Lavandula x intermedia Emeric ex Loisel. ‘Budrovka’, an indigenous Croatian cultivar of lavandin. For that purpose the activity of ethanolic extracts of flowers, inflorescence stalks and leaves against thirty one strains of bacteria, yeasts, dermatophytes and moulds were studied using both the agar well diffusion and broth dilution assays. Among the investigated extracts found to be effective against a broad ...

Flujo vaginal. Etiología en una unidad intermedia de salud

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Luz Marina Alzate

1987-12-01

Full Text Available En una serie de 584 mujeres que consultaron por flujo vaginal a una Unidad Intermedia de Salud se halló que la entidad más frecuente era la vaginosis bacteriana (vaginitis inespecífica o por Gardnerella vaginalis con un 34,8% seguida por la candidiasis (9,4% y la tricomoniasis (43%; hubo también infecciones mixtas (7,2%; la candidiasis, la tricomoniasis y las infecciones mixtas, pero no la vaginosis bacteriana, fueron más frecuentes en mujeres embarazadas; se hace énfasis en la facilidad de diagnosticar vaginosis bacteriana en el laboratorio corriente con base en criterios de muy fácil determinación.

Ramalina farinacea (L. Ach. ve Usnea intermedia (A.Massal. Jatta Likenlerinin Antimikrobiyal Aktiviteleri Üzerine Araştırmalar

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Neslihan ŞİRİN

2015-04-01

Full Text Available Bu çalışmada Bursa-Uludağ Milli Park civarından toplanan Ramalina farinacea (L. Ach. ve Usnea intermedia (A. Massal. Jatta likenlerinin Bacillus cereus ATCC 7064, Bacillus licheniformis ATCC 14580, Citrobacter freundii ATCC 8090, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25992, Klebsiella oxytoca ATCC 8724, Listeria innocua ATCC 33090, Proteus vulgaris ATCC 8427, Pseudomonas aeruginosa ATCC 27853, Salmonella enteridis ATCC 13076, Salmonella typhimurium CCM 5445, Shigella flexneri ATCC 12022, Staphylococcus aureus ATCC 6538P, Staphylococcus epidermidis ATCC 3699, Streptococcus pyogenes ATCC 19615, Yersinia pestis ATCC 19428, Candida albicans ATCC 90028, Candida glabrata ATCC 90030, Candida lipolytica ATCC 8660, Candida parapsilosis ATCC 22019, Candida tropicalis ATCC 4563, Cryptococcus neoformans ATCC 32045, Debaryomyces hansenii DSM 70238,  Saccharomyces cerevisiae ATCC 9796 türlerine karşı antimikrobiyal aktiviteleri araştırılmıştır.Bulgulara göre; Ramalina farinacea (L. Ach. likeninin en yüksek antimikrobiyal aktivitesi Salmonella enteritidis ATCC 13076 ve Candida albicans ATCC 90028 türlerine karşı bulunurken, Usnea intermedia (A. Massal Jatta likeninin en yüksek antimikrobiyal aktivitesi Streptococcus pyogenes ATCC 19615 ve Candida albicans ATCC 90028 türlerinde ölçülmüştür. Liken türlerinin diğer tüm mikroorganizmalara karşı farklı seviyelerde antimikrobiyal aktivite gösterdikleri saptanmıştır.Anahtar Kelimeler: Antimikrobiyal Aktivite, Ramalina farinacea, Usnea intermedia

Expression and immunological cross-reactivity of LALP3, a novel astacin-like metalloprotease from brown spider (Loxosceles intermedia) venom.

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Morgon, Adriano M; Belisario-Ferrari, Matheus R; Trevisan-Silva, Dilza; Meissner, Gabriel O; Vuitika, Larissa; Marin, Brenda; Tashima, Alexandre K; Gremski, Luiza H; Gremski, Waldemiro; Senff-Ribeiro, Andrea; Veiga, Silvio S; Chaim, Olga M

2016-01-01

Loxosceles spiders' venom comprises a complex mixture of biologically active toxins, mostly consisting of low molecular mass components (2-40 kDa). Amongst, isoforms of astacin-like metalloproteases were identified through transcriptome and proteome analyses. Only LALP1 (Loxosceles Astacin-Like protease 1) has been characterized. Herein, we characterized LALP3 as a novel recombinant astacin-like metalloprotease isoform from Loxosceles intermedia venom. LALP3 cDNA was cloned in pET-SUMO vector, and its soluble heterologous expression was performed using a SUMO tag added to LALP3 to achieve solubility in Escherichia coli SHuffle T7 Express LysY cells, which express the disulfide bond isomerase DsbC. Protein purification was conducted by Ni-NTA Agarose resin and assayed for purity by SDS-PAGE under reducing conditions. Immunoblotting analyses were performed with specific antibodies recognizing LALP1 and whole venom. Western blotting showed linear epitopes from recombinant LALP3 that cross-reacted with LALP1, and dot blotting revealed conformational epitopes with native venom astacins. Mass spectrometry analysis revealed that the recombinant expressed protein is an astacin-like metalloprotease from L. intermedia venom. Furthermore, molecular modeling of LALP3 revealed that this isoform contains the zinc binding and Met-turn motifs, forming the active site, as has been observed in astacins. These data confirmed that LALP3, which was successfully obtained by heterologous expression using a prokaryote system, is a new astacin-like metalloprotease isoform present in L. intermedia venom. Copyright © 2016 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

Agomelatine, a MT1/MT2 melatonergic receptor agonist with serotonin 5-HT2C receptor antagonistic properties, suppresses Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages.

Science.gov (United States)

Hyeon, Jin-Yi; Choi, Eun-Young; Choe, So-Hui; Park, Hae Ryoun; Choi, Jeom-Il; Choi, In Soon; Kim, Sung-Jo

2017-10-01

This study was performed in an attempt to examine the influence of agomelatine in mitigating the generation of proinflammatory mediators in RAW264.7 murine macrophages exposed to lipopolysaccharide (LPS) obtained from Prevotella intermedia, a gram-negative anaerobic bacterium that is related with various types of periodontal diseases, and the molecular mechanisms behind its effects. LPS from P. intermedia strain ATCC 25611 was prepared employing the conventional phenol-water procedure. Conditioned culture media were analyzed for the levels of nitric oxide (NO), interleukin-1β (IL-1β) and IL-6. Real-time PCR analysis was carried out to determine the mRNA levels of inducible NO synthase (iNOS), IL-1β, IL-6 and SOCS1. Protein expression levels were evaluated by immunoblot test. NF-κB-dependent SEAP reporter assay was performed using a reporter cell line. DNA-binding activities of NF-κB subunits were analyzed utilizing the ELISA-based kits. Agomelatine was found to down-regulate significantly the generation of iNOS-derived NO, IL-1β and IL-6 as well as the expression of their mRNAs in cells activated with P. intermedia LPS. Agomelatine decreased NF-κB-dependent SEAP release caused by P. intermedia LPS. Agomelatine did not inhibit NF-κB transcription induced by LPS at the level of IκB-α degradation. Instead, LPS-induced nuclear translocation and DNA binding of NF-κB p50 subunit was blocked by agomelatine. P. intermedia LPS-elicited activation of STAT1 and STAT3 was reduced notably by co-treatment with agomelatine. Agomelatine showed a tendency to enhance mRNA level of SOCS1 in LPS-activated cells as well. Agomelatine merits further evaluation to reveal its usefulness on the host modulation of periodontal disease. Copyright © 2017 Elsevier Ltd. All rights reserved.

The complete genome sequencing of Prevotella intermedia strain OMA14 and a subsequent fine-scale, intra-species genomic comparison reveal an unusual amplification of conjugative and mobile transposons and identify a novel Prevotella-lineage-specific repeat.

Science.gov (United States)

Naito, Mariko; Ogura, Yoshitoshi; Itoh, Takehiko; Shoji, Mikio; Okamoto, Masaaki; Hayashi, Tetsuya; Nakayama, Koji

2016-02-01

Prevotella intermedia is a pathogenic bacterium involved in periodontal diseases. Here, we present the complete genome sequence of a clinical strain, OMA14, of this bacterium along with the results of comparative genome analysis with strain 17 of the same species whose genome has also been sequenced, but not fully analysed yet. The genomes of both strains consist of two circular chromosomes: the larger chromosomes are similar in size and exhibit a high overall linearity of gene organizations, whereas the smaller chromosomes show a significant size variation and have undergone remarkable genome rearrangements. Unique features of the Pre. intermedia genomes are the presence of a remarkable number of essential genes on the second chromosomes and the abundance of conjugative and mobilizable transposons (CTns and MTns). The CTns/MTns are particularly abundant in the second chromosomes, involved in its extensive genome rearrangement, and have introduced a number of strain-specific genes into each strain. We also found a novel 188-bp repeat sequence that has been highly amplified in Pre. intermedia and are specifically distributed among the Pre. intermedia-related species. These findings expand our understanding of the genetic features of Pre. intermedia and the roles of CTns and MTns in the evolution of bacteria. © The Author 2015. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

Desarrollo curricular, conciencia ambiental y tecnologia para estudiantes de intermedia: Una investigacion en accion

Science.gov (United States)

Rodriguez Ramos, Teresita

Se llevó a cabo una investigación en acción con los propósitos de 1) documentar las relaciones de las tecnologías de la información y la comunicación en las clases de ciencias de escuela intermedia como elemento de apoyo cuando se aborda el tema ambiental y sus conceptos pertinentes, a partir de las observaciones de la investigadora, así como las entrevistas y diarios reflexivos de los estudiantes de una escuela intermedia en la zona metropolitana, y luego 2) diseñar una unidad instruccional sobre el tema ambiental que integre actividades tecnologías para el curso de ciencias de la escuela intermedia según el modelo PROCIC y las observaciones que hayan iniciado los estudiantes participantes. Finalmente, se plantearon las implicaciones educativas para el currículo del Programa de Ciencias al instrumentar este modelo de unidad mediante PROCIC, e integrado la tecnología y el tema ambiental. Los hallazgos se analizaron y se categorizaron de acuerdo con las preguntas de investigación. El hallazgo principal de la investigación aborda las cuatro relaciones centrales en las que se articula la utilización de las tecnologías y sus aplicaciones en la clase de ciencias. Estas cuatro relaciones que recogen la posición de los estudiantes son: 1) Perspectiva de los estudiantes hacia la tecnología. 2) Participación de los estudiantes en los aspectos docentes. 3) Aprendizaje estudiantil sobre el ambiente, y 4) Conciencia ambiental en relación con la vida diaria. Estas relaciones ponen de manifiesto,cómo se plantea en las implicaciones, la necesidad de más investigación en acción en la sala de clases, la importancia—como tema transversal—de la conciencia ambiental mediante la tecnología al construir conocimientos significativos dentro y fuera de la escuela, asó como, valorar la investigación y la dialogicidad en la sala de clases como actividades que obligan al reexamen de la práctica didáctica en su formas curriculares de objetivos, recursos

Intermedia transfer factors for fifteen toxic pollutants released to air basins in California

Energy Technology Data Exchange (ETDEWEB)

McKone, T.E.; Daniels, J.I. [Lawrence Livermore National Lab., CA (United States); Chiao, F.F.; Hsieh, D.P.H. [Univ. of California, Davis, CA (United States)

1993-10-01

This report provides a summary definition of the intermedia-transfer factors (ITFs). Methods are discussed for estimating these parameters in the absence of measured values, and the estimation errors inherent in these estimation methods are considered. A detailed summary is provided of measured and estimated ITF values for fifteen air contaminants. They include: 1,3 butadiene; cadmium; cellosolve; cellosolve acetate; chloroform; di-2-ethylhexylphthalate; 1,4-dioxame; hexachlorobenzene; inorganic arsenic; inorganic lead; nickel; tetrachloroethylene; toluene; toluene-2,4-diisocyanate; and 1,3-xylene. Recommendations are made regarding the expected value and variance in these values for use in exposure models.

Interleukin 10–Dominant Immune Response and Increased Risk of Cutaneous Leishmaniasis After Natural Exposure to Lutzomyia intermedia Sand Flies

Science.gov (United States)

Carvalho, Augusto M.; Cristal, Juqueline R.; Muniz, Aline C.; Carvalho, Lucas P.; Gomes, Regis; Miranda, José C.; Barral, Aldina; Carvalho, Edgar M.; de Oliveira, Camila I.

2015-01-01

Background. Leishmaniasis is caused by parasites transmitted to the vertebrate host by infected sand flies. During transmission, the vertebrate host is also inoculated with sand fly saliva, which exerts powerful immunomodulatory effects on the host's immune response. Methods. We conducted a prospective cohort analysis to characterize the human immune response to Lutzomyia intermedia saliva in 264 individuals, from an area for cutaneous leishmaniasis (CL) caused by Leishmania braziliensis. Results. Antibodies were found in 150 individuals (56.8%); immunoglobulin G1 and G4 were the predominant subclasses. Recall responses to salivary gland sonicate showed elevated production of interleukin 10 (IL-10), interleukin 13, interferon γ, CXCL9, and CCL2 compared with controls. CD4+CD25+ T cells, including Foxp3+ cells, were the main source of IL-10. L. braziliensis replication was increased (P intermedia sand flies skews the human immune response, facilitating L. braziliensis survival in vitro, and increases the risk of developing CL. PMID:25596303

Spatial distribution of Munida intermedia and M. sarsi (crustacea: Anomura) on the Galician continental shelf (NW Spain): Application of geostatistical analysis

Science.gov (United States)

Freire, J.; González-Gurriarán, E.; Olaso, I.

1992-12-01

Geostatistical methodology was used to analyse spatial structure and distribution of the epibenthic crustaceans Munida intermedia and M. sarsi within sets of data which had been collected during three survey cruises carried out on the Galician continental shelf (1983 and 1984). This study investigates the feasibility of using geostatistics for data collected according to traditional methods and of enhancing such methodology. The experimental variograms were calculated (pooled variance minus spatial covariance between samples taken one pair at a time vs. distance) and fitted to a 'spherical' model. The spatial structure model was used to estimate the abundance and distribution of the populations studied using the technique of kriging. The species display spatial structures, which are well marked during high density periods and in some areas (especially northern shelf). Geostatistical analysis allows identification of the density gradients in space as well as the patch grain along the continental shelf of 16-25 km diameter for M. intermedia and 12-20 km for M. sarsi. Patches of both species have a consistent location throughout the different cruises. As in other geographical areas, M. intermedia and M. sarsi usually appear at depths ranging from 200 to 500 m, with the highest densities in the continental shelf area located between Fisterra and Estaca de Bares. Althouh sampling was not originally designed specifically for geostatistics, this assay provides a measurement of spatial covariance, and shows variograms with variable structure depending on population density and geographical area. These ideas are useful in improving the design of future sampling cruises.

Differential Midgut Attachment of Leishmania (Viannia braziliensis in the Sand Flies Lutzomyia (Nyssomyia whitmani and Lutzomyia (Nyssomyia intermedia

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Rodrigo P. Soares

2010-01-01

Full Text Available The interaction between Leishmania and sand flies has been demonstrated in many Old and New World species. Besides the morphological differentiation from procyclic to infective metacyclic promastigotes, the parasite undergoes biochemical transformations in its major surface lipophosphoglycan (LPG. An upregulation of β-glucose residues was previously shown in the LPG repeat units from procyclic to metacyclic phase in Leishmania (Viannia braziliensis, which has not been reported in any Leishmania species. LPG has been implicated as an adhesion molecule that mediates the interaction with the midgut epithelium of the sand fly in the Subgenus Leishmania. These adaptations were explored for the first time in a species from the Subgenus Viannia, L. (V. braziliensis with its natural vectors Lutzomyia (Nyssomyia intermedia and Lutzomyia (Nyssomyia whitmani. Using two in vitro binding techniques, phosphoglycans (PGs derived from procyclic and metacyclic parasites were able to bind to the insect midgut and inhibit L. braziliensis attachment. Interestingly, L. braziliensis procyclic parasite attachment was ∼11-fold greater in the midgut of L. whitmani than in L. intermedia. The epidemiological relevance of L. whitmani as a vector of American Cutaneous Leishmaniasis (ACL in Brazil is discussed.

Side effects of Deferasirox Iron Chelation in Patients with Beta Thalassemia Major or Intermedia

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Murtadha Al-Khabori

2013-03-01

Full Text Available Objectives: Chelating agents remain the mainstay in reducing the iron burden and extending patient survival in homozygous beta-thalassemia but adverse and toxic effects may increase with the institution and long term use of this essential therapy. This study aimed to estimate the incidence of deferasirox (DFX side effects in patients with thalassemia major or intermedia.Methods: A retrospective study of 72 patients (mean age: 20.3±0.9 yrs; 36 male, 36 female with thalassemia major or intermedia treated at Sultan Qaboos University Hospital, Oman, was performed to assess the incidence of side effects related to deferasirox over a mean of 16.7 month follow-up period.Results: Six patients experienced rashes and 6 had gastro-intestinal upset. DFX was discontinued in 18 patients for the following reasons: persistent progressive rise(s in serum creatinine (7 patients; 40% mean serum creatinine rise from baseline, feeling unwell (2, severe diarrhea (1, pregnancy (1, death unrelated to chelator (2 and rise in serum transaminases (2. Three patients were reverted to desferoxamine and deferiprone combination therapy as DFX was no longer biochemically effective after 18 months of therapy. There was no correlation between baseline serum ferritin and serum creatinine or a rise in serum creatinine. Cardiac MRI T2* did not change with DFX therapy. However, there was an improvement in liver MRI T2* (p=0.013.Conclusion: Renal side effects related to deferasirox appear to be higher than those reported in published clinical trials. Further larger studies are required to confirm these findings.

Antimicrobial susceptibility pattern of Brachyspira intermedia isolates from European layers.

Science.gov (United States)

Verlinden, Marc; Boyen, Filip; Pasmans, Frank; Garmyn, An; Haesebrouck, Freddy; Martel, An

2011-09-01

A broth microdilution method was used to determine the antimicrobial susceptibility of 20 Brachyspira intermedia isolates obtained from different layer flocks in Belgium and The Netherlands between 2008 and 2010. The antimicrobial agents used were tylosin, tilmicosin, tiamulin, valnemulin, doxycycline, and lincomycin. The minimal inhibitory concentration (MIC) distribution patterns of tylosin, tilmicosin, lincomycin, and doxycycline were bimodal, demonstrating acquired resistance against doxycycline in three strains, against the macrolides in two strains, and against lincomycin in one strain. The MICs of tiamulin and valnemulin showed a monomodal distribution, but with tailing toward the higher MIC values, possibly suggesting low-level acquired resistance in six isolates. Sequencing revealed a G1058C mutation in the 16S rRNA gene in all doxycycline-resistant strains. The strain resistant to tylosin, tilmicosin, and lincomycin had an A2058T mutation in the 23S rRNA gene.

Periodontitis in cardiovascular disease patients with or without Marfan syndrome--a possible role of Prevotella intermedia.

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Jun-ichi Suzuki

Full Text Available BACKGROUND: Although periodontitis is a risk factor for cardiovascular disease (CVD, the influence of periodontitis on Marfan syndrome (MFS with CVD is unclear. The aim of this study was to assess the relationship between periodontal bacterial burden and MSF with CVD. METHODS AND RESULTS: The subjects were patients with MFS with CVD (n = 47; age and gender matched non-MFS CVD patients (n = 48 were employed as controls. Full-mouth clinical measurements, including number of teeth, probing of pocket depth (PD, bleeding on probing (BOP and community periodontal index (CPI were recorded. We also evaluated the existence of three periodontal pathogens, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Prevotella intermedia using polymerase chain reaction assays. Serum antibody titers against the pathogens were also measured. We revealed that MFS with CVD patients had periodontitis more frequently than the age and gender matched non-MFS CVD control subjects. MFS with CVD patients had significantly severer periodontitis, fewer remaining teeth and deeper PD compared to the non-MFS CVD controls. Furthermore, the serum antibody titer level against Prevotella intermedia was significantly lower in MFS plus CVD patients compared to the non-MFS CVD patients. CONCLUSION: Periodontitis may influence the pathophysiology of cardiovascular complications in MFS patients. A specific periodontal pathogen might be a crucial therapeutic target to prevent CVD development.

Ethanol and Protein from Ethanol Plant By-Products Using Edible Fungi Neurospora intermedia and Aspergillus oryzae.

Science.gov (United States)

Bátori, Veronika; Ferreira, Jorge A; Taherzadeh, Mohammad J; Lennartsson, Patrik R

2015-01-01

Feasible biorefineries for production of second-generation ethanol are difficult to establish due to the process complexity. An alternative is to partially include the process in the first-generation plants. Whole stillage, a by-product from dry-mill ethanol processes from grains, is mostly composed of undegraded bran and lignocelluloses can be used as a potential substrate for production of ethanol and feed proteins. Ethanol production and the proteins from the stillage were investigated using the edible fungi Neurospora intermedia and Aspergillus oryzae, respectively. N. intermedia produced 4.7 g/L ethanol from the stillage and increased to 8.7 g/L by adding 1 FPU of cellulase/g suspended solids. Saccharomyces cerevisiae produced 0.4 and 5.1 g/L ethanol, respectively. Under a two-stage cultivation with both fungi, up to 7.6 g/L of ethanol and 5.8 g/L of biomass containing 42% (w/w) crude protein were obtained. Both fungi degraded complex substrates including arabinan, glucan, mannan, and xylan where reductions of 91, 73, 38, and 89% (w/v) were achieved, respectively. The inclusion of the current process can lead to the production of 44,000 m(3) of ethanol (22% improvement), around 12,000 tons of protein-rich biomass for animal feed, and energy savings considering a typical facility producing 200,000 m(3) ethanol/year.

Molecular cloning and functional characterization of borneol dehydrogenase from the glandular trichomes of Lavandula x intermedia.

Science.gov (United States)

Sarker, Lukman S; Galata, Mariana; Demissie, Zerihun A; Mahmoud, Soheil S

2012-12-15

Several varieties of Lavandula x intermedia (lavandins) are cultivated for their essential oils (EOs) for use in cosmetic, hygiene and personal care products. These EOs are mainly constituted of monoterpenes including camphor, which contributes an off odor reducing the olfactory appeal of the oil. We have recently constructed a cDNA library from the glandular trichomes (the sites of EO synthesis) of L. x intermedia plants. Here, we describe the cloning of a borneol dehydrogenase cDNA (LiBDH) from this library. The 780 bp open reading frame of the cDNA encoded a 259 amino acid short chain alcohol dehydrogenase with a predicted molecular mass of ca. 27.5 kDa. The recombinant LiBDH was expressed in Escherichia coli, purified by Ni-NTA agarose affinity chromatography, and functionally characterized in vitro. The bacterially produced enzyme specifically converted borneol to camphor as the only product with K(m) and k(cat) values of 53 μM and 4.0 × 10(-4) s(-1), respectively. The LiBDH transcripts were specifically expressed in glandular trichomes of mature flowers indicating that like other Lavandula monoterpene synthases the expression of this gene is regulated in a tissue-specific manner. The cloning of LiBDH has far reaching implications in improving the quality of Lavandula EOs through metabolic engineering. Copyright © 2012 Elsevier Inc. All rights reserved.

Liver transplantation from a deceased donor with β-thalassemia intermedia is not contraindicated: A case report.

Science.gov (United States)

Gumus, Ersin; Abbasoglu, Osman; Tanyel, Cahit; Gumruk, Fatma; Ozen, Hasan; Yuce, Aysel

2017-05-01

The use of extended criteria donors who might have previously been deemed unsuitable is an option to increase the organ supply for transplantation. This report presents a pediatric case of a successful liver transplantation from a donor with β-thalassemia intermedia. A patient, 6-year-old female, with a diagnosis of cryptogenic liver cirrhosis underwent deceased donor liver transplantation from a thalassemic donor. Extreme hyperferritinemia was detected shortly after transplantation. The most probable cause of hyperferritinemia was iron overload secondary to transplantation of a hemosiderotic liver. Hepatocellular injury due to acute graft rejection might have contributed to elevated ferritin levels by causing release of stored iron from the hemosiderotic liver graft. Iron chelation and phlebotomy therapies were started simultaneously in the early postoperative period to avoid iron-related organ toxicity and transplant failure. Follow-up with monthly phlebotomies after discharge yielded a favorable outcome with normal transplant functions. Thalassemia intermedia patients can be candidates of liver donors to decrease pretransplant waitlist mortality. After transplantation of a hemosiderotic liver, it is important to monitor the recipient in terms of iron overload and toxicity. Early attempts to lower iron burden including chelation therapy and/or phlebotomy should be considered to avoid organ toxicity and transplant failure. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Survival and development of reintroduced Cattleya intermedia plants related to abiotic factors and herbivory at the edge and in the interior of a forest fragment in South Brazil

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Delio Endres Júnior

2018-06-01

Full Text Available ABSTRACT Biotic and abiotic factors, such as luminosity, temperature, air humidity, and herbivory, can affect the establishment of reintroduced plants in natural habitats. This study evaluated the effects of these factors on the survival and growth of Cattleya intermedia plants reintroduced into a forest fragment in South Brazil. Plants of C. intermedia were obtained from in vitro seed germination in asymbiotic culture. Eighty-eight plants were reintroduced at both the forest edge and forest interior. Plants with greater shoot heights and number of leaves and pseudobulbs suffered more damage from herbivores at the edge. There were no significant differences in morphometric parameters between damaged and non-damaged plants in the interior. Tenthecoris bicolor, Helionothrips errans, Ithomiola nepos, Molomea magna and Coleoptera larvae damaged C. intermedia. Luminosity was higher at the edge, while air humidity and temperature were the same in both environments. Herbivory associated with abiotic factors increased plant mortality in the interior, while abiotic factors were determinative of plant survival at the edge. Luminosity is important to the survival of reintroduced epiphytic orchids, and herbivory affects the success of reintroduction.

Biologia de Lutzomyia intermedia (Lutz & Neiva, 1912 e Lutzomyia longipalpis (Lutz & Neiva, 1912 (Diptera, Psychodidae em condições experimentais: II. Influência de fatores ambientais no comportamento das formas imaturas e adultas Biology of Lutzomyia intermedia (Lutz & Neiva, 1912 and Lutzomyia longipalpis (Lutz & Neiva, 1912 (Diptera, Psychodidae in experimental conditions: II. Influence of environmental factors in the behavior of immature stages and adults

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Elizabeth F. Rangel

1987-09-01

Full Text Available Com o objetivo de ampliar os conheciemntos sobre a biologia de Lutzomyia intermedia e Lutzomya longipalpis, mantidos em colônias autônomas no laboratório, apresentamos dados referentes a alterações em seu comportamento determinadas por influência de fatores ambientais. L. longipalpis foi mais fácil de criar, mais produtiva e mais resitente ás variações das condições ambientais; suga a qualquer hora do dia, enquanto que L. intermedia prefere fazê-lo ao crepúsculo e à noite, quando também ocorrem masi freqüentemente as desovas e as ecdises dos adultos das duas espécies. As fases imaturas de ambas as espécies resistem à imersão na água por até 1 hora e a baixa temperatura de 5ºC por até 6 horas.Proceeding on our studies on the biology of Lutzomyia intermedia and Lutzomyia longipalpis in closed colonies in the laboratory, we here present our observations on changes in their behavior caused by environmental conditions. L. longipaldis was easier to breed, more productive and more resistant to environmental changes; it feeds at any time of the day or night, while L. intermedia prefers to do it at sunset and at night, the preferencial time for oviposition and ecdysis of adults of both species. The immature stages of both species resisted to immersion in water for up to 1 hour and low temperature (5ºC for 1 to 6 hours.

Identification of two CiGADs from Caragana intermedia and their transcriptional responses to abiotic stresses and exogenous abscisic acid.

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Ji, Jing; Zheng, Lingyu; Yue, Jianyun; Yao, Xiamei; Chang, Ermei; Xie, Tiantian; Deng, Nan; Chen, Lanzhen; Huang, Yuwen; Jiang, Zeping; Shi, Shengqing

2017-01-01

stress. This study showed that two CiGAD s cloned from C. intermedia are closely related to homologs in another legume, soybean. CiGAD2 expression was much higher than that of CiGAD1 in bark, indicating that CiGAD2 might participate in the process of secondary growth in woody plants. Multiple stresses, interestingly, showed that Zn and heat stresses had the strongest effects on CiGAD1 expression, suggesting that CiGAD1 plays important roles in the responses to Zn and heat stresses. Additionally, these two genes might be involved in ABA dependent pathway during stress. This result provides important information about the role of GAD s in woody plants' responses to environmental stresses.

Species-specific ecological niche modelling predicts different range contractions for Lutzomyia intermedia and a related vector of Leishmania braziliensis following climate change in South America.

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McIntyre, Shannon; Rangel, Elizabeth F; Ready, Paul D; Carvalho, Bruno M

2017-03-24

Before 1996 the phlebotomine sand fly Lutzomyia neivai was usually treated as a synonym of the morphologically similar Lutzomyia intermedia, which has long been considered a vector of Leishmania braziliensis, the causative agent of much cutaneous leishmaniasis in South America. This report investigates the likely range changes of both sand fly species in response to a stabilisation climate change scenario (RCP4.5) and a high greenhouse gas emissions one (RCP8.5). Ecological niche modelling was used to identify areas of South America with climates currently suitable for each species, and then the future distributions of these climates were predicted based on climate change scenarios. Compared with the previous ecological niche model of L. intermedia (sensu lato) produced using the GARP algorithm in 2003, the current investigation modelled the two species separately, making use of verified presence records and additional records after 2001. Also, the new ensemble approach employed ecological niche modelling algorithms (including Maximum Entropy, Random Forests and Support Vector Machines) that have been widely adopted since 2003 and perform better than GARP, as well as using a more recent climate change model (HadGEM2) considered to have better performance at higher resolution than the earlier one (HadCM2). Lutzomyia intermedia was shown to be the more tropical of the two species, with its climatic niche defined by higher annual mean temperatures and lower temperature seasonality, in contrast to the more subtropical L. neivai. These different latitudinal ranges explain the two species' predicted responses to climate change by 2050, with L. intermedia mostly contracting its range (except perhaps in northeast Brazil) and L. neivai mostly shifting its range southwards in Brazil and Argentina. This contradicts the findings of the 2003 report, which predicted more range expansion. The different findings can be explained by the improved data sets and modelling methods. Our

Evaluation of Antioxidant Potential of Lavandula x intermedia Emeric ex Loisel. 'Budrovka': A Comparative Study with L. angustifolia Mill.

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Blažeković, Biljana; Vladimir-Knežević, Sanda; Brantner, Adelheid; Štefan, Maja Bival

2010-01-01

A Croatian indigenous cultivar of lavandin, Lavandula x intermedia 'Budrovka' (Lamiaceae) was studied for the phenolic acids, flavonoids, anthocyanins, procyanidins and total tannins, as well as total polyphenols content, in the flower, inflorescence stalk and leaf ethanolic extracts. Antioxidant potentials on these plant part extracts were assessed by the DPPH free radical scavenging activity, iron chelating activity, reducing power, lipid peroxidation inhibition properties and total antioxi...

Detection of Porphyromonas endodontalis, Porphyromonas gingivalis and Prevotella intermedia in primary endodontic infections in a Chinese population.

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Cao, H; Qi, Z; Jiang, H; Zhao, J; Liu, Z; Tang, Z

2012-08-01

To assess the prevalence of three black-pigmented bacterial species (Porphyromonas endodontalis, Porphyromonas gingivalis and Prevotella intermedia) using microarray technology in root canals of teeth associated with primary endodontic infections in a Chinese population. Microbial samples were taken from root canals of 80 teeth with pulp necrosis and primary endodontic infections in a Chinese population. DNA extracted from the samples was amplified by PCR with universal bacterial primers based on 16S rRNA gene sequences, and the products hybridized with the microarrays in which the specific oligonucleotide probes were added. The results of hybridization were screened by a confocal laser scanner. Pearson chi-square test and the two-sided Fisher exact test were used to analyse whether a significant association existed between the species and symptoms as well as in co-existence of two target organisms by a statistical software package (SAS 8.02). The 16S rRNA gene microarray detected at least one of the three test species in 76% of the study teeth. P. endodontalis, P. gingivalis and P. intermedia were found in 50%, 33% and 45%, respectively. A significant association was found in the presence of the pair P. endodontalis / P. gingivalis (P endodontalis (P endodontalis and P. gingivalis was also associated with the presence of a sinus tract (Pendodontalis were associated with the presence of sinus tract and abscess formation. © 2012 International Endodontic Journal.

Ethanol and Protein from Ethanol Plant By-Products Using Edible Fungi Neurospora intermedia and Aspergillus oryzae

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Veronika Bátori

2015-01-01

Full Text Available Feasible biorefineries for production of second-generation ethanol are difficult to establish due to the process complexity. An alternative is to partially include the process in the first-generation plants. Whole stillage, a by-product from dry-mill ethanol processes from grains, is mostly composed of undegraded bran and lignocelluloses can be used as a potential substrate for production of ethanol and feed proteins. Ethanol production and the proteins from the stillage were investigated using the edible fungi Neurospora intermedia and Aspergillus oryzae, respectively. N. intermedia produced 4.7 g/L ethanol from the stillage and increased to 8.7 g/L by adding 1 FPU of cellulase/g suspended solids. Saccharomyces cerevisiae produced 0.4 and 5.1 g/L ethanol, respectively. Under a two-stage cultivation with both fungi, up to 7.6 g/L of ethanol and 5.8 g/L of biomass containing 42% (w/w crude protein were obtained. Both fungi degraded complex substrates including arabinan, glucan, mannan, and xylan where reductions of 91, 73, 38, and 89% (w/v were achieved, respectively. The inclusion of the current process can lead to the production of 44,000 m3 of ethanol (22% improvement, around 12,000 tons of protein-rich biomass for animal feed, and energy savings considering a typical facility producing 200,000 m3 ethanol/year.

α:Non–α and Gγ:Aγ globin chain ratios in thalassemia intermedia patients treated with hydroxyurea

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Abbas Najjari

2014-05-01

Conclusions: Improvement in α:non-α ratio and consequent decrease of free α-globin chain might be the cause of beneficial effects of hydroxyurea therapy. Two patients who felt better didn't show significant increase in their fetal hemoglobin level, and this is in contradiction with the hypothesis claiming that the HbF level increase is the cause of such therapeutic effect. In spite of the unclear mechanism of action of this drug, hydroxyurea therapy had noticeable impacts on thalassemia intermedia and also sickle cell disease and even patients suffering from thalassemia major.

Gravitropism in caulonemata of the moss Pottia intermedia

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Chaban, C. I.; Kern, V. D.; Ripetskyj, R. T.; Demkiv, O. T.; Sack, F. D.

1998-01-01

The gravitropism of caulonemata of Pottia intermedia is described and compared with that of other mosses. Spore germination produces primary protonemata including caulonemata which give rise to buds that form the leafy moss plant, the gametophore. Primary caulonemata are negatively gravitropic but their growth and the number of filaments are limited in the dark. Axenic culture of gametophores results in the production of secondary caulonemata that usually arise near the leaf base. Secondary protonemata that form in the light are agravitropic. Secondary caulonemata that form when gametophores are placed in the dark for several days show strong negative gravitropism and grow well in the dark. When upright caulonemata are reorientated to the horizontal or are inverted, upward bending can be detected after 1 h and caulonemata reach the vertical within 1-2 d. Clear amyloplast sedimentation occurs 10-15 minutes after horizontal placement and before the start of upward curvature. This sedimentation takes place in a sub-apical zone. Amyloplast sedimentation also takes place along the length of upright and inverted Pottia protonemata. These results support the hypothesis that amyloplast sedimentation functions in gravitropic sensing since sedimentation occurs before gravitropism in Pottia and since the location and presence of a unique sedimentation zone is conserved in all four mosses known to gravitropic protonomata.

Blood feeding patterns of Nyssomyia intermedia and Nyssomyia neivai (Diptera, Psychodidae in a cutaneous leishmaniasis endemic area of the Ribeira Valley, State of São Paulo, Brazil

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Ana Maria Marassa

2013-09-01

Full Text Available Introduction The aim of this study was to identify the blood feeding sources of Nyssomyia intermedia (Ny. intermedia and Nyssomyia neivai (Ny. neivai, which are Leishmania vectors and the predominant sandfly species in the Ribeira Valley, State of São Paulo, Brazil, an endemic area for cutaneous leishmaniasis. Methods Specimens were captured monthly between February 2001 and December 2003 on a smallholding and a small farm situated in the Serra district in the Iporanga municipality. The blood meals of 988 engorged females were tested using the avidin-biotin immunoenzymatic enzyme-linked immunosorbent assay (ELISA. Seven blood meal sources were investigated: human, dog, chicken, bovine, pig, horse and rat. Results The results showed that among the females that fed on one or more blood sources, the respective percentages for Ny. intermedia and Ny. neivai, respectively, were as follows: human (23% and 36.8%, pig (47.4% and 26.4%, chicken (25.7% and 36.8% and dog (3.9% and 0%, and the differences in the blood sources between the two species were statistically significant (p = 0.043. Conclusions Both species had predominant reactivity for one or two blood sources, and few showed reactivity indicating three or four sources. Many different combinations were observed among the females that showed reactivity for more than one source, which indicated their opportunistic habits and eclecticism regarding anthropic environmental conditions.

Studies on the biosynthesis of lignins and their production in plant cell cultures. [Forsythia intermedia; Podophyllum hexandrum; Podophyllum peltatum

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Abdul-Rahman, M.M.

1988-01-01

Phytochemical analysis of Forsythia intermedia tissues has demonstrated the presence of lignins of the dibenzylbutyrolactone and dioxabicyclo(3,3,0)octane classes, together with their O-glucosides. Lignin distribution in different parts of the part, and variation with the season has been recorded. Cell suspension cultures from F. intermedia were developed. These also synthesized lignins, though not the full range as found in the fresh tissues. Culture lines synthesizing either matairesinol 4{prime}-O-glucoside and major lignin components were established. These cultures rapidly metabolized exogenous lignins without producing detectable lignin transformation products. The seasonal variation in aryltetralin lactone levels in young plants Podophyllum hexandrum and P. peltatum has been investigated. Cell cultures of the plants were established, but no lignins were detectable in them. However, a protocol for micropropagation via embryoid production was obtained. Feeding experiments in P. hexandrum plants showed that matairesinol was a precursor of both podophyllotoxin and 4{prime}-demethylpodophyllotoxin, thus indicating it to be a common precursor of the trimethoxy and hydroxydimethoxy series of lignins in this plant. Precursor feeding experiments with {sup 3}H/{sup 14}C-labeled coniferyl alcohol showed an unexpected increase in {sup 3}H/{sup 14}C ratio when incorporated into both Forsythia and Podophyllum lignins.

La tendencia ambiental urbana en ciudades intermedias. Una propuesta metodológica

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Pinzón Botero María Victoria

2011-11-01

Full Text Available Desde las últim as décadas, es evidente la preocupación m undial por los im pactos ambientales negativos que ponen en riesgo la sostenibilidad urbana y su calidad ambiental. Diferentes estudios exponen las variables más importantes para minim izar estos impactos ambientales, y en este sentido, este trabajo de investigación muestra cómo a partir de un diseño metodológico más sencillo con 27 variables am bientales estratégicas, es posible evaluar la tendencia am biental urbana de ciudades intermedias. Este grupo priorizado de variables trabajadas en el marco de una gestión compartida, orientará el desarrollo de la ciudad hacia una estructura territorial más sostenible, como reto para la planificación ambiental actual y con visión de futuro.

BIOLOGICALLY ACTIVE SUBSTANCES OF LAVANDULA X INTERMEDIA EMERIC EX LOISEL (LAMIACEAE

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A. E. Paliy

2016-01-01

Full Text Available Data about qualitative and quantitative composition of biologically active substances (volatile and phenolic compounds in water- ethanol extract of Lavandula x intermedia Emeric ex Loisel (Lamiaceae cv. ‘Bora’ bred in Nikitsky Botanical Garden are presented in the article. Concentration of volatile compounds in Lavandin extract was 398 mg/dm3 and 51 components were identified. Main volatiles in Lavandin cv. «Bora» extract were linalyl acetate (36,9% and linalool (33,5%. Content of phenolic compounds in water- ethanol extract of Lavandin was 945 mg/dm3 and 14 components were found out. Among the variety of Lavandin cv. ‘Bora’ phenolic compounds luteolin-7-O-glycoside and п-coumaric acid are predominated. The conclusion about possible use of Lavandin cv. ‘Bora’ for creation of food, cosmetic, therapeutic and preventive products is made.

Histopathological hoof laminar changes in horses with Pituitary Pars Intermedia Adenoma: cases report

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L. M. Laskoski

2015-10-01

Full Text Available ABSTRACTLaminitis in horses is often associated with endocrine disorders, especially the pituitary pars intermedia dysfunction (PPID in older animals. Morphologic exams of the laminar tissue of the hoof were performed in two horses with suspected PPID, with no clinical signs of laminitis. Changes compatible with laminitis of endocrine origin were observed, such as rounding of the nuclei of the basal cells, thinning and stretching of the secondary epidermal laminae and tissue proliferation. PPID horses with no clinical signs of laminitis may be affected by lesions of the laminar tissue of the hoof that compromise the integrity of the dermal-epidermal junction and may develop clinical symptoms of the disease. It has been suggested that the development stage of endocrine laminitis is longer, but further studies should be conducted to confirm it.

Determination of Five Chemical Markers in DF Formula, the Herbal Composition of Ephedra intermedia, Rheum palmatum, and Lithospermum erythrorhizon, Using High-performance Liquid Chromatography-ultraviolet Detection.

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Jeong, Birang; Roh, Jong Seong; Yoon, Michung; Yoon, Yoosik; Shin, Soon Shik; Cho, Hyun Joo; Kwon, Yong Soo; Yang, Heejung

2018-01-01

DF formula is a herbal preparation comprised three medicinal herbs, namely, Ephedra intermedia , Rheum palmatum , and Lithospermum erythrorhizon , which is being used for the treatment of obesity and liver fibrosis in Korean local clinics. Since the abovementioned three herbs exist with different proportions in DF formula and their chemical markers have different physiochemical properties; it is quite challenging to develop an analytical methodology for the determination of these chemical markers. For the analysis of the three herbs, five chemicals, (+)-pseudoephedrine (1) and (-)-ephedrine (2) for E. intermedia , aloe-emodin (3), and chrysophanol (4) for R. palmatum , and shikonin (5) for L. erythrorhizon , were selected for method validation of DF formula, and the analytical conditions were optimized and validated using high-performance liquid chromatography coupled with an ultraviolet detector (HPLC-UV). The specificities for the five compounds 1-5 were determined by their UV absorption spectra (1-4: 215 nm and 5: 520 nm). Their calibration curves showed good linear regressions with high correlation coefficient values ( R 2 > 0.9997). The limits of detection of these five markers were in the range 0.4-2.1 ng/mL, with the exception of 5 (12.7 ng/mL). The intraday variability for all the chemical markers was less than a Relative standard deviation (RSD) of 3%, except for 5 (RSD = 12.6%). In the case of interday analysis, 1 (1.0%), 2 (3.1%), and 4 (3.7%) showed much lower variabilities (RSD chemical markers showed good recoveries with good accuracies in the range of 90%-110%. The developed HPLC-UV method for the determination of the five chemical markers of the components of DF formula was validated. DF formula, the herbal composition of Ephedra intermedia , Rheum palmatum and Lithospermum erythrorhizon Five chemical markers in DF formula were (+)-pseudoephedrine (1) and (-)-ephedrine (2) for E. intermedia , aloe-emodin (3) and chrysopanol (4) for R. palmatum , and

Protective effects of fermented honeybush (Cyclopia intermedia) extract (HU-018) against skin aging: a randomized, double-blinded, placebo-controlled study.

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Choi, Sun Young; Hong, Ji Yeon; Ko, Eun Jung; Kim, Beom Joon; Hong, Sung-Woon; Lim, Mi Hyoung; Yeon, Sung Hum; Son, Rak Ho

2018-02-01

Oxidative stress and photodamage resulting from ultraviolet radiation exposure play key roles in skin aging. Fermented Cyclopia intermedia, which is used to brew honeybush tea, exerts antioxidant and anti-wrinkle effects by inhibiting reactive oxygen species production and downregulating matrix metalloproteinase activity. This randomized, double-blinded, placebo-controlled study aimed to evaluate the efficacy and safety of fermented honeybush (Cyclopia intermedia) extract (HU-018) for skin rejuvenation. 120 Korean subjects with crow's feet wrinkles were randomized to receive either low-dose extract (400 mg/day), high-dose extract (800 mg/day), or placebo (negative control, only dextran) for 12 weeks. Wrinkles were evaluated using JANUS ® and PRIMO pico ® . Skin elasticity, hydration and transepidermal water loss were measured. Global skin wrinkle grade was significantly improved in both low-dose and high-dose groups compared to placebo group, as well as for skin hydration and elasticity. Both the low- and high-dose groups showed significantly decreased TEWL compared to the placebo group. There were no adverse effects during the entire study period. Our data indicate that HU-018 is effective for improving skin wrinkles, elasticity, and hydration. Therefore, daily supplementation with fermented honeybush could be helpful for protecting against skin aging.

Postembryonic development of Loxosceles intermedia Mello-Leitão, 1934, L. laeta (Nicolet, 1849 and L. gaucho Gertsch, 1967 (Araneae; Sicariidae breeding under conditions of monospecific diet

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Emanuel Marques da Silva

2005-05-01

Full Text Available The influence of monospecific feeding on the post-embryonic development of L. intermedia, L. laeta and L. gaucho was evaluated. Two hundred and ten spiderlings were individualized in containers (120 ml, 105 being fed with Tenebrio molitor larvae and 105 with Pycnoscelus surinamensis nymphs. In the three species, the number of molts varied according to the diet. The number of spiders that reached maturity was lower in L. gaucho. In L. intermedia, the duration of the post-embryonic period was greater when larvae are fed. Mortality was higher in the second instar in the three species, the highest frequency being registered for L. gaucho. The data provided evidence that monospecific feeding influenced the post-embryonic development of the studied species. This influence might intensified by specific characteristics such as origin, habits and habitats.

[125I]Bolton-Hunter neuropeptide-Y-binding sites on folliculo-stellate cells of the pars intermedia of Xenopus laevis: A combined autoradiographic and immunocytochemical study

International Nuclear Information System (INIS)

De Rijk, E.P.; Cruijsen, P.M.; Jenks, B.G.; Roubos, E.W.

1991-01-01

It has previously been established that neuropeptide-Y (NPY) is a potent inhibitor of alpha MSH release from the pars intermedia of the amphibian Xenopus laevis. The location of binding sites for NPY in the pars intermedia of the pituitary has now been studied with light microscopic autoradiography, using a dispersed cell labeling method with the specific NPY receptor ligand [ 125 I]Bolton-Hunter NPY. The majority of radioactive labeling was associated with folliculo-stellate cells; the percentage of labeling as well as the mean number of grains were approximately 5 times higher for folliculo-stellate cells than for melanotropes. An excess of nonlabeled NPY drastically reduced radiolabeling of folliculo-stellate cells, but had no effect on the degree of labeling of melanotropes. These results show that folliculo-stellate cells of X. laevis possess specific binding sites for NPY and indicate that NPY exerts its inhibitory action on the release of alpha MSH in an indirect fashion, by acting on the folliculo-stellate cells

Double heterozygocity for hemoglobin C and beta thalassemia dominant: A rare case of thalassemia intermedia

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Alexandra Agapidou

2018-01-01

Full Text Available Beta thalassemia dominant results from mutations in the β globin chain gene resulting in the production of elongated, highly unstable beta globin chains. Several such mutations have been described and in a heterozygous state they may confer a phenotype more severe than that of β thalassemia trait and lead to a clinical syndrome of thalassemia intermedia and its associated complications such as extramedullary hemopoiesis, bone disease, endocrinopathies and iron overload even in the absence of transfusion. In this report we present a case of double heterozygocity for HbC and β thalassemia dominant leading to a series of complications that were treated successfully once the correct diagnosis was made.

CÓMO SE NEGOCIA UNA POSICIÓN DE SUJETO INTERMEDIA: MUCHACHOS ENTRE ONCE Y CATORCE AÑOS, MASCULINIDADES Y EDUCACIÓN ESCOLAR

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Ann Phoenix

2002-01-01

Full Text Available Este ensayo pone a discusión la ciencia (generalizada en las escuelas de Londres según la cual existe una oposición entre la masculinidad y la realización de tareas escolares, que tiene fuertes efectos en la subjetividad de lo masculino del joven. Así considera las formas en que éstos asisten a las escuelas de Londres y negocian lo que ellos consideran las contradicciones que involucra el tratar de reconciliar el hacer las tareas escolares con ser aceptado por los otros en su condición masculina. El documento se centra particularmente en la forma como los muchachos negocian una posición de sujeto intermedia, en un intento de asegurarse que trabajaron lo suficiente para no reprobar, pero no tanto como para llamar la atención de los otros, por no ser "propiamente masculinos". El texto también afirma que estas contradicciones tienen una carga emocional, de modo que los jóvenes se esfuerzan en negociar lo que ellos consideran una posición intermedia, pues esta es la posición de sujeto que les resulta menos problemática ocupar.

[Phylogenetic diversity of microorganisms associated with the deep-water sponge Baikalospongia intermedia].

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Kalyzhnaya, O V; Itskovich, V B

2014-07-01

The diversity of bacteria associated with deep-water sponge Baikalospongia intermedia was evaluated by sequence analysis of 16S rRNA genes from two sponge samples collected in Lake Baikal from depths of 550 and 1204 m. A total of 64 operational taxonomic units, belonging to nine bacterial phyla, Proteobacteria (classes Alphaproteobacteria,. Betaproteobacteria, Gammaproteobacteria, and Deltaproteobacteria), Actinobacteria, Planctomycetes, Cloroflexi, Verrucomicrobia, Acidobacteria, Chlorobi, and Nitrospirae, including candidate phylum WS5, were identified. Phylogenetic analysis showed that the examined communities contained phylotypes exhibiting homology to uncultured bacteria from different lake ecosystems, freshwater sediments, soil and geological formations. Moreover, a number of phylotypes were relative to psychrophilic, methane-oxidizing, sulfate-reducing bacteria, and to microorganisms resistant to the influence of heavy metals. It seems likely that the unusual habitation conditions of deep-water sponges contribute to the taxonomic diversity of associated bacteria and have an influence on the presence of functionally important microorganisms in bacterial communities.

Toward intelligent information sysytem

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Onodera, Natsuo

"Hypertext" means a concept of a novel computer-assisted tool for storage and retrieval of text information based on human association. Structure of knowledge in our idea processing is generally complicated and networked, but traditional paper documents merely express it in essentially linear and sequential forms. However, recent advances in work-station technology have allowed us to process easily electronic documents containing non-linear structure such as references or hierarchies. This paper describes concept, history and basic organization of hypertext, and shows the outline and features of existing main hypertext systems. Particularly, use of the hypertext database is illustrated by an example of Intermedia developed by Brown University.

Isolation and functional characterization of a methyl jasmonate-responsive 3-carene synthase from Lavandula x intermedia.

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Adal, Ayelign M; Sarker, Lukman S; Lemke, Ashley D; Mahmoud, Soheil S

2017-04-01

A methyl jasmonate responsive 3-carene synthase (Li3CARS) gene was isolated from Lavandula x intermedia and functionally characterized in vitro. Lavenders produce essential oils consisting mainly of monoterpenes, including the potent antimicrobial and insecticidal monoterpene 3-carene. In this study we isolated and functionally characterized a leaf-specific, methyl jasmonate (MeJA)-responsive monoterpene synthase (Li3CARS) from Lavandula x intermedia. The ORF excluding transit peptides encoded a 64.9 kDa protein that was expressed in E. coli, and purified with Ni-NTA agarose affinity chromatography. The recombinant Li3CARS converted GPP into 3-carene as the major product, with K m and k cat of 3.69 ± 1.17 µM and 2.01 s -1 respectively. Li3CARS also accepted NPP as a substrate to produce multiple products including a small amount of 3-carene. The catalytic efficiency of Li3CARS to produce 3-carene was over ten fold higher for GPP (k cat /K m = 0.56 µM -1 s -1 ) than NPP (k cat /K m = 0.044 µM -1 s -1 ). Production of distinct end product profiles from different substrates (GPP versus NPP) by Li3CARS indicates that monoterpene metabolism may be controlled in part through substrate availability. Li3CARS transcripts were found to be highly abundant in leaves (16-fold) as compared to flower tissues. The transcriptional activity of Li3CARS correlated with 3-carene production, and was up-regulated (1.18- to 3.8-fold) with MeJA 8-72 h post-treatment. The results suggest that Li3CARS may have a defensive role in Lavandula.

Lavandin (Lavandula × intermedia Emeric ex Loiseleur) essential oil from Spain: determination of aromatic profile by gas chromatography-mass spectrometry, antioxidant and lipoxygenase inhibitory bioactivities.

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Carrasco, Alejandro; Martinez-Gutierrez, Ramiro; Tomas, Virginia; Tudela, Jose

2016-01-01

Lavandin (Lavandula × intermedia Emeric ex Loiseleur) essential oils (EOs), from Abrial, Super and Grosso cultivars, cultivated and extracted in the South East of Spain, were analysed by using GC/MS to determine their composition, in both relative (peak area) and absolute (using standard curves) concentrations. Linalool (34-47%), linalyl acetate (17-34%), camphor (4-9%) and eucalyptol (3-7%) were determined as the main molecules. This characterisation was completed with the enantioselective gas chromatography, where ( - )-linalool, (+)-camphor and ( - )-linalyl acetate were determined as the main components. Antioxidant activity was evaluated positively by several methods: activity against free radicals, chelating and reducing power, probably due to linalool and linalyl acetate. Mild inhibitory activity on lipoxygenase was observed supporting potential anti-inflammatory activity, mainly due to linalool and camphor. These properties support the potential use of L. × intermedia essential oils as natural cosmetic and natural pharmaceutical ingredient to fight several skin diseases.

CARDIAC FUNCTION AND IRON CHELATION IN THALASSEMIA MAJOR AND INTERMEDIA: A REVIEW OF THE UNDERLYING PATHOPHYSIOLOGY AND APPROACH TO CHELATION MANAGEMENT

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Athanasios Aessopos

2009-07-01

Full Text Available Heart disease is the leading cause of mortality and one of the main causes of morbidity in beta-thalassemia. Patients with homozygous thalassemia may have either a severe phenotype which is usually transfusion dependent or a milder form that is thalassemia intermedia.  The two main factors that determine cardiac disease in homozygous β thalassemia are the high output state that results from chronic tissue hypoxia, hypoxia-induced compensatory reactions and iron overload.  The high output state playing a major role in thalassaemia intermedia and the iron load being more significant in the major form. Arrhythmias, vascular involvement that leads to an increased pulmonary vascular resistance and an increased systemic vascular stiffness and valvular abnormalities also contribute to the cardiac dysfunction in varying degrees according to the severity of the phenotype.  Endocrine abnormalities, infections, renal function and medications can also play a role in the overall cardiac function.  For thalassaemia major, regular and adequate blood transfusions and iron chelation therapy are the mainstays of management. The approach to thalassaemia intermedia, today, is aimed at monitoring for complications and initiating, timely, regular transfusions and/or iron chelation therapy.  Once the patients are on transfusions, then they should be managed in the same way as the thalassaemia major patients.  If cardiac manifestations of dysfunction are present in either form of thalassaemia, high pre transfusion Hb levels need to be maintained in order to reduce cardiac output and appropriate intensive chelation therapy needs to be instituted.  In general recommendations on chelation, today, are usually made according to the Cardiac Magnetic Resonance findings, if available.  With the advances in the latter technology and the ability to tailor chelation therapy according to the MRI findings as well as the availability of three iron chelators, together with

Are light traps baited with kairomones effective in the capture of Lutzomyia longipalpis and Lutzomyia intermedia? An evaluation of synthetic human odor as an attractant for phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae).

Science.gov (United States)

Andrade, Andrey J; Andrade, Mateus R; Dias, Edelberto S; Pinto, Mara C; Eiras, Alvaro E

2008-06-01

Phlebotomine sand flies are often captured with human bait and/or light traps, either with or without an animal bait. More recently, synthetic attractants have been used as bait in traps to improve the capture of phlebotomine sand flies as well as other insects of medical and veterinary importance. The aim of the present study was to evaluate the effects of the kairomone 1-octen-3-ol (octenol) and the synthetic human odor BG-Mesh Lure (BGML--lactic acid, caproic acid and ammonia) baited in modified CDC light traps on the capture of phlebotomine sand flies. The experiments followed the 5x5 Latin square design. Among the species caught, Lutzomyia intermedia apparently presented a dose-dependent response to octenol. The response obtained with the BGML, alone or in combination with octenol (5 mg/h), indicated some degree of attractiveness of these baits to different phlebotomine sand fly species. Octenol seems to be more attractive to L. intermedia than to Lutzomyia longipalpis, while the BGML presented a higher success in capturing L. longipalpis. When the components of the BGML were used separately, there was no increase in catching the female of L. intermedia. Apparently, there was no synergistic effect between the octenol and the BGML. In conclusion, the octenol and the BGML were demonstrated to be possible baits to attract some phlebotomine sand fly species.

Transfusion regimens in thalassemia intermedia

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Z. Karakas

2011-12-01

Full Text Available Thalassemia intermedia (TI is a heterogeneous disease, in terms of both clinical manifestations and underlying molecular defects. Some TI patients are asymptomatic until adult life, whereas others are symptomatic from early childhood. In contrast with patients with Thalassemia major (TM, the severity of anemia is less and the patients do not require transfusions during at least the first few years of life. Many patients with TI, especially older ones, have been exposed to the multiple long-term effects of chronic anemia and tissue hypoxia and their compensatory reactions, including enhanced erythropoiesis and increased iron absorption. Bone marrow expansion and extramedullary hematopoiesis lead to bone deformities and liver and spleen enlargement. Therapeutic strategies in TI are not clear and different criteria are used to decide the initiation of transfusion and chelation therapy, modulation of fetal hemoglobin production, and hematopoietic stem cell transplantation on an individual basis. The clinical picture of well-treated TM patients with regular transfusionchelation therapy is better from TI patients who have not received adequate transfusion therapy. There is a significant role of early blood transfusion to prevent and treat complications commonly associated with TI, such as extramedullary erythropoiesis and bone deformities, autoimmune hemolytic anemia, leg ulcers, gallstones, pseudoxantoma elasticum, hyperuricosuria, gout and pulmonary hypertension, which are rarely seen in thalassemia major. Nowadays, indications of transfusion in patients with TI are chronic anemia (Hb < 7 g/dL, bone deformities, growth failure, extramedullary erythropoiesis, heart failure, pregnancy and preparation for surgical procedures. Conclusion: Adequate (regular or tailored transfusion therapy is an important treatment modality for increasing the quality of life in patients with thalassemia intermedia during childhood

In Vitro Antibacterial and Antifungal Activity of Lavandula x intermedia Emeric ex Loisel. ‘Budrovka’

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Sanda Vladimir-Knezevic

2011-05-01

Full Text Available This study aimed to evaluate the in vitro antibacterial and antifungal activities of Lavandula x intermedia Emeric ex Loisel. ‘Budrovka’, an indigenous Croatian cultivar of lavandin. For that purpose the activity of ethanolic extracts of flowers, inflorescence stalks and leaves against thirty one strains of bacteria, yeasts, dermatophytes and moulds were studied using both the agar well diffusion and broth dilution assays. Among the investigated extracts found to be effective against a broad spectrum of microorganisms, the flower extract was considered to be the most potent one. Linalool and rosmarinic acid, as the most abundant constituents found, are very likely major contributors to the observed antimicrobial effects. The results suggest that flowers of lavandin ‘Budrovka’ could serve as a rich source of natural terpene and polyphenol antimicrobial agents.

Biodegradation of diuron by an endophytic fungus Neurospora intermedia DP8-1 isolated from sugarcane and its potential for remediating diuron-contaminated soils

OpenAIRE

Wang, Yanhui; Li, Honghong; Feng, Guojun; Du, Liangwei; Zeng, Dongqiang

2017-01-01

A diuron-degrading endophyte DP8-1 was isolated from sugarcane root grown in diuron-treated soil in the present study. The endophyte was identified as Neurospora intermedia based on the morphological characteristics and sequence analysis. The fermentation parameters including temperature, pH, inoculation size, carbon source, and initial diuron concentration were also investigated for the optimization of degradation efficiency. The results indicated that strain DP8-1 was capable of degrading u...

Evidence of mutualism between two periodontal pathogens: co-operative haem acquisition by the HmuY haemophore of Porphyromonas gingivalis and the cysteine protease interpain A (InpA) of Prevotella intermedia.

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Byrne, D P; Potempa, J; Olczak, T; Smalley, J W

2013-06-01

Haem (iron protoporphyrin IX) is both an essential growth factor and a virulence regulator of the periodontal pathogens Porphyromonas gingivalis and Prevotella intermedia, which acquire it through the proteolytic degradation of haemoglobin and other haem-carrying plasma proteins. The haem-binding lipoprotein HmuY haemophore and the gingipain proteases of P. gingivalis form a unique synthrophic system responsible for capture of haem from haemoglobin and methaemalbumin. In this system, methaemoglobin is formed from oxyhaemoglobin by the activities of gingipain proteases and serves as a facile substrate from which HmuY can capture haem. This study examined the possibility of cooperation between HmuY and the cysteine protease interpain A (InpA) of Pr. intermedia in the haem acquisition process. Using UV-visible spectroscopy and polyacrylamide gel electrophoresis, HmuY was demonstrated to be resistant to proteolysis and so able to cooperate with InpA to extract haem from haemoglobin, which was proteolytically converted to methaemoglobin by the protease. Spectroscopic pH titrations showed that both the iron(II) and iron(III) protoporphyrin IX-HmuY complexes were stable over the pH range 4-10, demonstrating that the haemophore could function over a range of pH that may be encountered in the dental plaque biofilm. This is the first demonstration of a bacterial haemophore working in conjunction with a protease from another bacterial species to acquire haem from haemoglobin and may represent mutualism between P. gingivalis and Pr. intermedia co-inhabiting the periodontal pocket. © 2013 John Wiley & Sons A/S. Published by Blackwell Publishing Ltd.

The effect of HBB:c.*+96T>C (3’UTR +1570 T>C on the mild b-thalassemia intermedia phenotype

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Türker Bilgen

2011-09-01

Full Text Available Hemoglobin beta (HBB:c.*+96T>C substitution is very rare among β-globin gene mutations and its clinical significance remains to be clarified. The present study aimed to investigate the role of HBB:c.*+96T>C in the β-thalassemia intermedia phenotype in a Turkish family. The proband and parents were screened for β-globin gene mutations via direct sequencing. Hematological and physical examination results were recorded, and correlated according to genotype. The proband was compound heterozygous for Cod 8 (-AA and HBB:c.*+96T>C, whereas his mother and father were heterozygous for Cod 8 (-AA and HBB:c.*+96T>C, respectively. The father had almost normal hematological findings, whereas the mother had the typical β-thalassemia trait phenotype. The proband was diagnosed as mild β-thalassemia intermedia based on hepatosplenomegaly and hematological findings. To the best of our knowledge this is the first report of HBB:c.*+96T>C mutation in a Turkish family. HBB:c.* 96T>C substitution is a very rare, but clinically relevant β-globin gene mutation. Additionally, we think that if 1 spouse is a carrier for β-globin gene mutation the other should be screened for silent mutations, such as HBB:c.*+96T>C mutation of the β-globin gene, even if she/he does not have any clinical or hematological signs of the β-thalassemia trait phenotype.

Single-cell Protein and Xylitol Production by a Novel Yeast Strain Candida intermedia FL023 from Lignocellulosic Hydrolysates and Xylose.

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Wu, Jiaqiang; Hu, Jinlong; Zhao, Shumiao; He, Mingxiong; Hu, Guoquan; Ge, Xiangyang; Peng, Nan

2018-05-01

Yeasts are good candidates to utilize the hydrolysates of lignocellulose, the most abundant bioresource, for bioproducts. This study aimed to evaluate the efficiencies of single-cell protein (SCP) and xylitol production by a novel yeast strain, Candida intermedia FL023, from lignocellulosic hydrolysates and xylose. This strain efficiently assimilated hexose, pentose, and cellubiose for cell mass production with the crude protein content of 484.2 g kg -1 dry cell mass. SCP was produced by strain FL023 using corncob hydrolysate and urea as the carbon and nitrogen sources with the dry cell mass productivity 0.86 g L -1  h -1 and the yield of 0.40 g g -1 sugar. SCP was also produced using NaOH-pretreated Miscanthus sinensis straw and corn steep liquor as the carbon and nitrogen sources through simultaneous saccharification and fermentation with the dry cell productivity of 0.23 g L -1  h -1 and yield of 0.17 g g -1 straw. C. intermedia FL023 was tolerant to 0.5 g L -1 furfural, acetic acid, and syringaldehyde in xylitol fermentation and produced 45.7 g L -1 xylitol from xylose with the productivity of 0.38 g L -1  h -1 and the yield of 0.57 g g -1 xylose. This study provides feasible methods for feed and food additive production from the abundant lignocellulosic bioresources.

Psycho-Cultural Analysis of Disaster Risk Attitudes in Situation Awareness

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2013-09-01

Ignore information - Seek information .595 B2 Warn people - Remain silent .741 B1 Attend warnings - Ignore warning .719...Trust warning siren .714 B5 Ignore information - Seek information .697 B1 Attend warnings - Ignore warning .638...Reduction and Climate Change Adaptation. Rugby , UK: Practical Action Publishing, ISBN 978-1-85339-786-8. UNISDR (2009). Terminology on Disaster Risk

A persistent cough as atypical clinical presentation of intrathoracic extramedullary hematopoiesis (EMH) in a female with thalassemia intermedia.

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Abdulla, Mohammad Aj; Yassin, Mohamed A; Abdelrazek, Mohamed; Mudawi, Deena; Ibrahim, Firyal; Soliman, Dina S; ElOmri, Halima; Nashwan, Abdulqadir J; Fernihough, Liam; De Sanctis, Vincenzo; Soliman, Ashraf T

2018-02-16

Extramedullary hematopoiesis (EMH) is a rare disorder, defined as the appearance of hematopoietic elements outside the bone marrow or peripheral blood. The most common sites of EMH are liver and spleen, but it has been documented in other organs such as the mediastinum, lymph nodes, breast, and central nervous system. EMH occurs as a compensatory mechanism for bone marrow dysfunction in severe thalassemia. We report a case of EMH presenting as a posterior mediastinal mass in a 34-year-old woman with thalassemia intermedia with chronic cough and shortness of breath on exertion. The diagnosis of EMH was confirmed by a CT-guided fine needle biopsy. All symptoms disappeared after surgical removal of the mass.

Caracterización de dos sistemas de abanicos aluviales húmedos en el Terciario de la Depresión Intermedia (Cuenca-Guadalajara)

OpenAIRE

Torres Pérez-Hidalgo, Trinidad José

1986-01-01

En este trabajo se comparan las características de dos abanicos aluviales del Primer Ciclo Neógeno (Ageniense superior-Aragoniense inferior) de la Depresion Intermedia. Estos abanicos tienen una serie de características comunes como: área fuente en la Cordillera Ibérica, períodos alternantes de funcinamiento de canales de baja y alta sinuosidad e interdigitación dista1 con depósitos lacustres de yesos bioturbados. Se diferencian en la orientación de paleocorrientes y presenc...

Comparing the Efficacy of Tadalafil Versus Placebo on Pulmonary Artery Systolic Pressure and Right Ventricular Function in Patients with Beta-Thalassaemia Intermedia.

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Jalalian, Rozita; Moghadamnia, Ali Akbar; Tamaddoni, Ahmad; Khafri, Soraya; Iranian, Mohammadreza

2017-07-01

Conventional oral therapies in the management of pulmonary hypertension in people without haemoglobinopathies are of limited value in thalassaemia patients because of toxicity and poor effectiveness. This study was conducted to assess the effect of tadalafil on pulmonary artery pressure and right ventricular systolic function in patients with beta-thalassaemia intermedia. Forty-four patients with beta-thalassaemia intermedia with pulmonary hypertension based on transthoracic echocardiography (TTE) were entered in the study. Patients with hepatic or renal insufficiency and also patients who were treated with organic nitrates or alpha-blockers were excluded. The patients were randomly divided into two groups (n=22) and they were treated for six weeks with tadalafil (40mg daily) or placebo. The pulmonary artery systolic pressure (PASP), tricuspid regurgitation velocity (TRV) and parameters related to systolic function of the right ventricle were measured by the TTE before and after treatment. Significant improvement in TRV (3.02±0.02 m/s-2.52±0.06 m/s), PASP (45.31±0.66 mmHg-34.26±1.15mmHg) and parameters related to systolic function of the right ventricle were observed in the group who received tadalafil compared to placebo (pintermedia. Likewise, tadalafil improved right ventricular systolic function in the patients. Copyright © 2016 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ). Published by Elsevier B.V. All rights reserved.

Caracterización de dos sistemas de abanicos aluviales húmedos en el Terciario de la Depresión Intermedia (Cuenca-Guadalajara)

OpenAIRE

de TORRES, T.; ZAPATA, J. L.

1986-01-01

En este trabajo se comparan las características de dos abanicos aluviales del Primer Ciclo Neógeno (Ageniense superior-Aragoniense inferior) de la Depresion Intermedia. Estos abanicos tienen una serie de características comunes como: área fuente en la Cordillera Ibérica, períodos alternantes de funcinamiento de canales de baja y alta sinuosidad e interdigitación distal con depósitos lacustres de yesos bioturbados. Se diferencian en la orientación de paleocorrientes y presencia en uno de ellos...

La Ciénaga de Córcoles: un área de sedimentación lacustre-palustre en el Mioceno (Aragoniense inferior-medio) de la Depresión Intermedia (Guadalajara)

OpenAIRE

Torres Pérez-Hidalgo, Trinidad José

1986-01-01

Las ciénagas de Córcoles son depósitos lacustres-palustres del terce Ciclo Neógeno de la Depresión Intermedia (Aragoniense inferior medio). Estuvieron distalmente relacionadas con el abanico aluvial, aunque rodeadas de un paisaje árido. las concentraciones singenéticas de metales sugiere que también tuvieron aportes de aguas termales.

Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae by in vitro techniques Indução e identificação de poliploides em Cattleya intermedia Lindl. (orchidaceae através de técnicas in vitro

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Paulo Artur Konzen Xavier de Mello e Silva

2000-03-01

Full Text Available The effect of colchicine on the protocorm-like bodies of Cattleya intermedia L. (Orchidaceae cultured in vitro was studied. Different concentrations of the drug (0.00, 0.05, 0.10 and 0.20% as well as two times of culture (4 or 8 days were tested to determine the best treatment for the induction of tetraploid plants. Of the 3 colchicine levels applied, 0.05 and 0.10% of drug treatments appeared to be effective on the production of mixoploids and tetraploids and could be further used in breeding programs. The suitability of stomatal area and stomata density as criteria for the distinction between diploids and tetraploids was also tested. The results suggested that tetraploid plants could be identified with a fair amount of certainty when the screening was based on the density of stomata.No presente trabalho, foi estudado o efeito da colchicina em corpos semelhantes a protocormos de Cattleya intermedia L. (Orchidaceae. Diferentes concentrações da droga (0,00; 0,05; 0,10 e 0,20%, bem como diferentes tempos de cultura (4 ou 8 dias foram testados para determinar o melhor tratamento para a indução de plantas tetraplóides. Dos três níveis de colchicina aplicados, os tratamentos com 0,05 e 0,10% da droga parecem ser mais efetivos na produção de mixoplóides e tetraplóides, sendo que tais tratamentos podem ser utilizados em programas de melhoramento. Como critério para distinção entre diplóides e tetraplóides, foram realizadas medidas de área de estômatos e densidade de estômatos das folhas. Os resultados sugerem que as plantas tetraplóides podem ser identificadas com elevado grau de certeza quando a seleção é realizada com base na densidade de estômatos.

Localization of amylin-like immunoreactivity in melanocyte-stimulating hormone-containing cells of the pars intermedia but not those of the pars distalis in the axolotl (Ambystoma mexicanum) pituitary.

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Suzuki, Hirohumi; Yamamoto, Toshiharu

2016-04-01

Immunohistochemical techniques were employed to investigate the distribution of amylin-like immunoreactivity in the axolotl (Ambystoma mexicanum) pituitary. Amylin-immunoreactive cells were observed in the pars intermedia, and these cells were found to be immunoreactive for α-melanocyte-stimulating hormone (αMSH) as well. In contrast, αMSH-immunoreactive cells in the pars distalis were immuno-negaitive for amylin. These light microscopic findings were confirmed by immunoelectron microscopy. Amylin-immunoreactive signals were located on the haloes of presumable secretory granules in association with αMSH-immunoreactive signals in the amylin-positive cells. However, in the pars distalis, the αMSH-positive cells did not contain amylin-immunoreactive secretory granules. Western blot analysis of axolotl pituitary extracts revealed the labeling of a protein band at approximately 10.5-kDa by the anti-rat amylin serum, which was not labeled by the anti-αMSH antibody. These findings indicate that amylin secreted from MSH-producing cells in the pars intermedia may modulate MSH secretion in an autocrine fashion and may participate in MSH functions such as fatty homeostasis together with MSH. Copyright © 2016 Elsevier GmbH. All rights reserved.

POLÍTICAS URBANAS PARA EL DESARROLLO Y LA INNOVACIÓN EN LAS CIUDADES INTERMEDIAS

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Henar Pascual Ruiz-Valdepeñas

2008-01-01

Full Text Available En los últimos años se asiste al fortalecimiento del papel de las ciudades intermedias al estimarsesu capacidad para lograr una mayor cohesión económica, social y territorial en Europa. Las políticas territoriales se orientan a reforzar sus ventajas competitivas y a potenciar la configuración y consolidación de redes de cooperación en las que las ciudades actúan como partes de un modelo policéntrico. La valoración de los impactos de los programas europeos en las ciudades y el análisis de las políticas y estrategias urbanas, ponen de relieve la naturaleza específica de los diversos niveles de intervención pública en el territorio y la multiplicidad de las interacciones que se generan entre ellos. En el nivel local de intervención, la planificación estratégica amplía el abanico de objetivos y actuaciones y aporta nuevos instrumentos que ponen el acento en el reforzamiento de los factores de competitividad urbana.

Uveítis intermedia: Experiencias terapéuticas Intermediate uveitis: Therapeutic experiences

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Sandra M. Borges Pérez

2001-12-01

Full Text Available Se realizó un estudio descriptivo prospectivo a 50 ojos (41 pacientes con el diagnóstico de uveítis intermedia durante un período de 3 años. Esta patología fue más frecuente en la mujer (58 %, en edades jóvenes (25 a 45 años y en plena actividad laboral. Se presentan complicaciones y dentro de ellas el edema macular cistoide constituye la causa que más afecta la visión. La mejoría de la agudeza visual fue más notable en los pacientes bajo régimen de tratamiento médico acompañado de tratamiento quirúrgico (40 %. El 50 % de los pacientes presentaron recidivas.A descriptive and prospective study of 50 eyes (41 patients with the diagnosis of intermediate uveitis was conducted during 3 years. This pathology was more frequent in women (58 %, in young patients (25-45 years old and among workers. Among the complications, the cystoid macular edema was the main cause of vision affections. The improvement of visual acuity was more remarkable in patients under medical and surgical treatment (40 %. 50 % of the patients had relapses.

Fábricas diagenéticas asociadas al paleokarst del techo de la Unidad Intermedia del Mioceno de la cuenca de Madrid

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Calvo, J. P.

1997-12-01

Full Text Available Carbonates and evaporites from the top of the Miocene Intermediate Unit of the Madrid Basin have set as host-rock for early karstification phenomena. The development of these karstic processes imply an important change in the depositional evolution of the basin. In these materials, complex diagenetic fabrics formed as a result of shallow meteoric diagenetic processes have been recognized, being dedolomitization and calcitization of gypsums the main diagenetic processes that affected the top of the Miocene Intermediate Unit. The high variability of diagenetic fabrics is thought to be mainly influenced by the original composition of sediments, the activity of microorganisms and the chemistry of diagenetic waters.Los materiales carbonáticos y evaporíticos del techo de la Unidad Intermedia del Mioceno de la Cuenca de Madrid han actuado como soporte de fenómenos de karstificación temprana, cuyo desarrollo ha supuesto un cambio significativo en la evolución del relleno de la cuenca. Dentro de estos materiales se han reconocido carbonatos con fábricas diagenéticas complejas resultado de procesos de diagénesis meteórica superficial. Los procesos predominantes en la evolución diagenética del encajante de la paleokarstificación del techo de la Unidad Intermedia son la dedolomitización y la calcitización/pseudomorfización de yesos. Factores como la composición original del sedimento, la influencia de microorganismos y la hidroquímica de los fluidos diagenéticos han determinado la gran variabilidad de las fábricas diagenéticas reconocidas.

Dominant modifiable risk factors for stroke in Ghana and Nigeria (SIREN: a case-control study

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Mayowa O Owolabi, ProfDrMed

2018-04-01

Full Text Available Summary: Background: Sub-Saharan Africa has the highest incidence, prevalence, and fatality from stroke globally. Yet, only little information about context-specific risk factors for prioritising interventions to reduce the stroke burden in sub-Saharan Africa is available. We aimed to identify and characterise the effect of the top modifiable risk factors for stroke in sub-Saharan Africa. Methods: The Stroke Investigative Research and Educational Network (SIREN study is a multicentre, case-control study done at 15 sites in Nigeria and Ghana. Cases were adults (aged ≥18 years with stroke confirmed by CT or MRI. Controls were age-matched and gender-matched stroke-free adults (aged ≥18 years recruited from the communities in catchment areas of cases. Comprehensive assessment for vascular, lifestyle, and psychosocial factors was done using standard instruments. We used conditional logistic regression to estimate odds ratios (ORs and population-attributable risks (PARs with 95% CIs. Findings: Between Aug 28, 2014, and June 15, 2017, we enrolled 2118 case-control pairs (1192 [56%] men with mean ages of 59·0 years (SD 13·8 for cases and 57·8 years (13·7 for controls. 1430 (68% had ischaemic stoke, 682 (32% had haemorrhagic stroke, and six (<1% had discrete ischaemic and haemorrhagic lesions. 98·2% (95% CI 97·2–99·0 of adjusted PAR of stroke was associated with 11 potentially modifiable risk factors with ORs and PARs in descending order of PAR of 19·36 (95% CI 12·11–30·93 and 90·8% (95% CI 87·9–93·7 for hypertension, 1·85 (1·44–2·38 and 35·8% (25·3–46·2 for dyslipidaemia, 1·59 (1·19–2·13 and 31·1% (13·3–48·9 for regular meat consumption, 1·48 (1·13–1·94 and 26·5% (12·9–40·2 for elevated waist-to-hip ratio, 2·58 (1·98–3·37 and 22·1% (17·8–26·4 for diabetes, 2·43 (1·81–3·26 and 18·2% (14·1–22·3 for low green leafy vegetable consumption, 1·89 (1·40–2·54 and 11·6% (6·6–16·7

On the re-emergence of motion and innovations in the Gábor Bódy's intermedia experiments

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Kamil Lipiński

2015-06-01

Full Text Available The article explores the main cases of Hungarian neo-avant-garde cinema in terms of re-emergence of visual material and the introduction of electronic innovations resulting in the transformation of reality. Among the research conducted in Béla Balázs Studio based on reframing the perception limits in the 1960s and the 1970s, the most innovative were Gábor Bódy's intermedia experiments. Along with launching K/3 subgroup within BBS, Bódy directed its objectives to reduce the material derived from reality and to visually intervene into media image electronically transforming the landscape of his films. Innovatively adapting the Sándor Weöres's verse, the film Narcissus and Psyche (1983 aimed at using hyper-real aesthetics to reframe the film reality. Starting from 1980, Bódy founded an international magazine Infermental to disseminate the advent of the video and electronics by creating a community drew on sharing news about emerging media image.

A randomized Phase I/II Trial of HQK-1001, an oral fetal globin gene inducer, in β–thalassaemia intermedia and HbE/β–thalassaemia

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Fucharoen, Suthat; Inati, Adlette; Siritanaratku, Noppadol; Thein, Swee Lay; Wargin, William C.; Koussa, Suzanne; Taher, Ali; Chaneim, Nattawara; Boosalis, Michael; Berenson, Ronald; Perrine, Susan P.

2014-01-01

β–thalassemia intermedia syndromes (BTI) cause hemolytic anemia, ineffective erythropoiesis, and widespread complications. Higher fetal globin expression within genotypes reduces globin imbalance and ameliorates anemia. Sodium 2,2 dimethylbutyrate (HQK-1001), an orally bioavailable short-chain fatty acid derivative, induces γ-globin expression experimentally and is well-tolerated in normal subjects. Accordingly, a randomized, blinded, placebo-controlled, Phase I/II trial was performed in 21 adult BTI patients (14 with HbE/β0 thalassemia and 7 with β+/β0 thalassemia intermedia, to determine effective doses for fetal globin induction, safety, and tolerability. HQK-1001 or placebo were administered once daily for 8 weeks at four dose levels (10, 20, 30, or 40 mg/kg/day), and subjects were monitored for laboratory and clinical events. Pharmacokinetic profiles demonstrated a t1/2 of 10–12 hours. Adverse events with HQK-1001 treatment were not significantly different from placebo treatment. Median HbF increased with the 20 mg/kg treatment doses above baseline levels by 6.6% and 0.44 g/dL (p <0.01) in 8/9 subjects; total hemoglobin (Hgb) increased by a mean of 1.1 gm/dL in 4/9 subjects. These findings identify a safe oral therapeutic which induces fetal globin in BTI. Further investigation of HQK-1001 with longer dosing to definitively evaluate its hematologic potential appears warranted. PMID:23530969

Paisajes del delito y la inseguridad en las ciudades intermedias

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Quim Bonastra

2011-02-01

Full Text Available Until very recently, phenomena like delinquency were only considered dysfunctions of the system. However, nowadays this phenomenon has become inherent to its way of functioning. This dynamic is influenced by factors such as the increasing polarization of developed economies or the enormous masses of displaced people, due to either economic or political reasons, from developing countries who, in rich countries, often fall in situations of precariousness. In the following text we will see how all these pieces are interrelated in an intermediate city, and we will analyse the fluctuation of violent or intimidating thefts, as well as the most common places where these are committed.Si hasta hace bien poco se había considerado que fenómenos como la delincuencia no eran más que disfunciones del sistema, en el mundo en el que vivimos este fenómeno se ha convertido en inherente a su manera de funcionar. Influyen en esta dinámica factores como la polarización creciente de las economías desarrolladas o las ingentes masas de desplazados, ya sea por razones económicas o políticas, de países en vías de desarrollo que, en los países ricos, caen las más de las veces en la precariedad. En el texto que sigue se verá como se engarzan todas estas piezas en una ciudad intermedia y se analizará la fluctuación de los robos con violencia o intimidación, así como los lugares típicos en los que éstos se cometen.DOWNLOAD THIS PAPER FROM SSRN: http://ssrn.com/abstract=1739302

Quesintuu y Umantuu: Sirenas y memoria andina Quesintuu y Umantuu: Sereia e memória andina Quesintuu and Umantuu: Sirens and Andean Memory

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Paola Revilla Orías

2012-12-01

Full Text Available En una aproximación a ciertas representaciones de sirenas que se encuentran en diferentes soportes coloniales del área andina, particularmente de la zona lacustre, este estudio indaga en su sentido polisémico. Se propone una lectura alternativa que parte de la identificación de un referente indígena poco conocido sobre dos seres femeninos y pisciformes del entorno del Lago Titicaca, cuya memoria se preserva y reactualiza por siglos en un plano de contenido subyacente al de la representación europea.Em uma abordagem sobre as representações das sereias que estão em diferentes meios da região andina colonial, particularmente na área lacustre, este estudo investiga o sentido polissêmico para propor uma leitura alternativa. Nesse sentido, esta pesquisa parte da identificação de alguns referentes indígenas pouco conhecidos sobre dois seres femininos e pisciformes do ambiente do Lago Titicaca, cuja memória é preservada e atualizada durante séculos em um plano subjacente ao da representação européia.This research analyzes the polysemic meanings and proposes an alternative interpretation to certain representations of Sirens found in a variety of colonial materials in the Andean region, particularly in the lacustrine region. This interpretation is based on the identification of some little known indigenous referent on two fishlike female beings from the surroundings of Lake Titicaca, whose memory has being preserved and rearticulated for centuries with content levels underlying European representation.

Epidemiology of pituitary pars intermedia dysfunction: A systematic literature review of clinical presentation, disease prevalence and risk factors.

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Ireland, J L; McGowan, C M

2018-05-01

Pituitary pars intermedia dysfunction (PPID) is caused by an age-related degenerative disease of dopaminergic neurones. Despite its importance in equine practice, available information regarding its epidemiology is limited. This systematic review aimed to assess published literature to evaluate available evidence regarding the clinical presentation, prevalence and risk factors for PPID in horses and ponies. Electronic database searches were undertaken using a range of terms, and English language publications published prior to August 2016 were included. Both authors independently reviewed screened papers for inclusion, extracted data, and assessed the quality of reporting using predefined criteria. Data were extracted using modified critically appraised topic data collection forms. Meta-analysis was not undertaken due to marked between-study variations. Following removal of duplicate records, of 358 published papers yielded by the search, 97 abstracts were screened for eligibility and 29 publications meeting inclusion criteria were included in the review. Most studies reviewed were case series or cross-sectional studies, with considerable variation in study populations and PPID case definition. Hypertrichosis and/or other hair coat abnormalities, laminitis and epaxial muscle wastage or muscle atrophy are the most frequently reported clinical signs, with prevalence of these signs increasing with increasing horse age. The most robust prevalence estimates for PPID were 21.2% in horses and ponies aged ≥15 years and 2.9% amongst the general equine population. Findings regarding breed and sex predispositions were equivocal and only increasing age has been identified as a significant risk factor for PPID. Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

El anticlinal y las discordancias de Pareja (Guadalajara): definición de las unidades cenozoicas de la Depresión Intermedia (provs. Cuenca y Guadalajara, España)

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Torres Pérez-Hidalgo, Trinidad José

2006-01-01

Este trabajo contempla las unidades representadas en la sección estratigráfica del Anticlinal la Pareja (Guadalajara, España) en la cuenca cenozoica denominada Depresión Intermedia. La columna estratigráfica de la zona permite identificar tres unidades principales separadas por discordancias angulares relacionables con fases tectónicas de la orogenia alpina. También se han diferenciado subunidades que, en su mayor parte, vienen definidas por rupturas sedimentarias ligadas a proces...

Hydroxyurea appears beneficial in patients with beta-thalassaemia major and intermedia

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S. F.S.A. Abdul Wahid

2007-06-01

Full Text Available Patients with severe inherited β-globin chain disorders may have milder illness if they produce high levels of fetal hemoglobin (HbF. Hydroxyurea (HU has been shown to enhance HbF levels in patients with sickle cell disease and may be useful in β-thalassemias. We administered HU to 13 patients with β-thalassemia intermedia or major, including 6 splenectomized patients. The patients received escalating doses (10 to 25 mg/kg/d of HU for around 2 years (median: 21 months, range: 8 - 55 months. Eleven patients responded with an increase in the pre-transfusion HbF levels, from a base line median of 8.0% (2.5 - 61.3% to 28.0% (6.6 - 49.2% and 40.7% (4.8 - 72.3% at 3 months and 18 months post-HU, respectively. A concomitant increment in median hemoglobin levels was noted at 1, 3 and 18 months of HU therapy. Six of 7 transfusion-dependent patients who had an increment of HbF (one with β-thalassemia major also had reduced transfusion requirement over the 2-year period of HU therapy. Response to HU was also shown by a reduction in spleen size. Apart from oral ulcers that resolved upon dose reduction of HU, no significant toxicity was noted. We conclude that increased HbF production in β-thalassemia patients, with an improvement in erythropoiesis, can be achieved using HU with minimal toxicity. (Med J Indones 2007; 16:78-83 Keywords: fetal hemoglobin (HbF, erythropoiesis, toxicity

A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom

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Trevisan-Silva, Dilza; Bednaski, Aline V.; Fischer, Juliana S.G.; Veiga, Silvio S.; Bandeira, Nuno; Guthals, Adrian; Marchini, Fabricio K.; Leprevost, Felipe V.; Barbosa, Valmir C.; Senff-Ribeiro, Andrea; Carvalho, Paulo C.

2017-01-01

Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10 kDa cutoff mass. Each of these was further fractioned and digested with trypsin (4 h), trypsin (18 h), pepsin (18 h), and chymotrypsin (18 h), then analyzed by MudPIT on an LTQ-Orbitrap XL ETD mass spectrometer fragmenting precursors by CID, HCD, and ETD. Aliquots of undigested samples were also analyzed. Our experimental design allowed us to apply spectral networks, thus enabling us to obtain meta-contig assemblies, and consequently de novo sequencing of practically complete proteins, culminating in a deep proteome assessment of the venom. Data are available via ProteomeXchange, with identifier PXD005523. PMID:28696408

Isorhamnetin inhibits Prevotella intermedia lipopolysaccharide-induced production of interleukin-6 in murine macrophages via anti-inflammatory heme oxygenase-1 induction and inhibition of nuclear factor-κB and signal transducer and activator of transcription 1 activation.

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Jin, J Y; Choi, E Y; Park, H R; Choi, J I; Choi, I S; Kim, S J

2013-12-01

Interleukin-6 (IL-6) is a key proinflammatory cytokine that has been considered to be important in the pathogenesis of periodontal disease. Therefore, host-modulatory agents directed at inhibiting IL-6 appear to be beneficial in terms of attenuating periodontal disease progression and potentially improving disease susceptibility. In the current study, we investigated the effect of the flavonoid isorhamnetin on the production of IL-6 in murine macrophages stimulated with lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in inflammatory periodontal disease, and its mechanisms of action. Lipopolysaccharide from P. intermedia ATCC 25611 was isolated using the standard hot phenol-water method. Culture supernatants were collected and assayed for IL-6. We used real-time PCR to quantify IL-6 and heme oxygenase-1 (HO-1) mRNA expression. The expression of HO-1 protein and the levels of signaling proteins were monitored using immunoblot analyses. The DNA-binding activity of nuclear factor-κB (NF-κB) was analyzed using ELISA-based assay kits. Isorhamnetin significantly down-regulated P. intermedia LPS-induced production of IL-6 as well as its mRNA expression in RAW264.7 cells. Isorhamnetin up-regulated the expression of HO-1 at both gene transcription and translation levels in cells stimulated with P. intermedia LPS. In addition, inhibition of HO-1 activity by tin protoporphyrin IX blocked the inhibitory effect of isorhamnetin on IL-6 production. Isorhamnetin failed to prevent LPS from activating either c-Jun N-terminal kinase or p38 pathways. Isorhamnetin did not inhibit NF-κB transcriptional activity at the level of inhibitory κB-α degradation. Isorhamnetin suppressed NF-κB signaling through inhibition of nuclear translocation and DNA binding activity of NF-κB p50 subunit and attenuated signal transducer and activator of transcription 1 signaling. Although further research is required to clarify the detailed mechanism of action, we propose

Beyond Chloride Brines: Variable Metabolomic Responses in the Anaerobic Organism Yersinia intermedia MASE-LG-1 to NaCl and MgSO4 at Identical Water Activity

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Schwendner, Petra; Bohmeier, Maria; Rettberg, Petra; Beblo-Vranesevic, Kristina; Gaboyer, Frédéric; Moissl-Eichinger, Christine; Perras, Alexandra K.; Vannier, Pauline; Marteinsson, Viggó T.; Garcia-Descalzo, Laura; Gómez, Felipe; Malki, Moustafa; Amils, Ricardo; Westall, Frances; Riedo, Andreas; Monaghan, Euan P.; Ehrenfreund, Pascale; Cabezas, Patricia; Walter, Nicolas; Cockell, Charles

2018-01-01

Growth in sodium chloride (NaCl) is known to induce stress in non-halophilic microorganisms leading to effects on the microbial metabolism and cell structure. Microorganisms have evolved a number of adaptations, both structural and metabolic, to counteract osmotic stress. These strategies are well-understood for organisms in NaCl-rich brines such as the accumulation of certain organic solutes (known as either compatible solutes or osmolytes). Less well studied are responses to ionic environments such as sulfate-rich brines which are prevalent on Earth but can also be found on Mars. In this paper, we investigated the global metabolic response of the anaerobic bacterium Yersinia intermedia MASE-LG-1 to osmotic salt stress induced by either magnesium sulfate (MgSO4) or NaCl at the same water activity (0.975). Using a non-targeted mass spectrometry approach, the intensity of hundreds of metabolites was measured. The compatible solutes L-asparagine and sucrose were found to be increased in both MgSO4 and NaCl compared to the control sample, suggesting a similar osmotic response to different ionic environments. We were able to demonstrate that Yersinia intermedia MASE-LG-1 accumulated a range of other compatible solutes. However, we also found the global metabolic responses, especially with regard to amino acid metabolism and carbohydrate metabolism, to be salt-specific, thus, suggesting ion-specific regulation of specific metabolic pathways. PMID:29535699

Beyond Chloride Brines: Variable Metabolomic Responses in the Anaerobic Organism Yersinia intermedia MASE-LG-1 to NaCl and MgSO4 at Identical Water Activity

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Petra Schwendner

2018-02-01

Full Text Available Growth in sodium chloride (NaCl is known to induce stress in non-halophilic microorganisms leading to effects on the microbial metabolism and cell structure. Microorganisms have evolved a number of adaptations, both structural and metabolic, to counteract osmotic stress. These strategies are well-understood for organisms in NaCl-rich brines such as the accumulation of certain organic solutes (known as either compatible solutes or osmolytes. Less well studied are responses to ionic environments such as sulfate-rich brines which are prevalent on Earth but can also be found on Mars. In this paper, we investigated the global metabolic response of the anaerobic bacterium Yersinia intermedia MASE-LG-1 to osmotic salt stress induced by either magnesium sulfate (MgSO4 or NaCl at the same water activity (0.975. Using a non-targeted mass spectrometry approach, the intensity of hundreds of metabolites was measured. The compatible solutes L-asparagine and sucrose were found to be increased in both MgSO4 and NaCl compared to the control sample, suggesting a similar osmotic response to different ionic environments. We were able to demonstrate that Yersinia intermedia MASE-LG-1 accumulated a range of other compatible solutes. However, we also found the global metabolic responses, especially with regard to amino acid metabolism and carbohydrate metabolism, to be salt-specific, thus, suggesting ion-specific regulation of specific metabolic pathways.

Differential accumulation of volatile terpene and terpene synthase mRNAs during lavender (Lavandula angustifolia and L. x intermedia) inflorescence development.

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Guitton, Yann; Nicolè, Florence; Moja, Sandrine; Valot, Nadine; Legrand, Sylvain; Jullien, Frédéric; Legendre, Laurent

2010-02-01

Despite the commercial importance of Lavandula angustifolia Mill. and L. x intermedia Emeric ex Loisel floral essential oils (EOs), no information is currently available on potential changes in individual volatile organic compound (VOC) content during inflorescence development. Calyces were found to be the main sites of VOC accumulation. The 20 most abundant VOCs could be separated into three sub-groups according to their patterns of change in concentration The three groups of VOCs sequentially dominated the global scent bouquet of inflorescences, the transition between the first and second groups occurring around the opening of the first flower of the inflorescence and the one between the second and third groups at the start of seed set. Changes in calyx VOC accumulation were linked to the developmental stage of individual flowers. Leaves accumulated a smaller number of VOCs which were a subset of those seen in preflowering inflorescences. Their nature and content remained constant during the growing season. Quantitative real time polymerase chain reaction assessments of the expression of two terpene synthase (TPS) genes, LaLIMS and LaLINS, revealed similar trends between their patterns of expression and those of their VOC products. Molecular and chemical analyses suggest that changes in TPS expression occur during lavender inflorescence development and lead to changes in EO composition. Both molecular data and terpene analysis support the findings that changes in biosynthesis of terpene occurred during inflorescence development.

Serum Prohepcidin Levels in Children with Thalassemia Major and Intermedia

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Celil Yılmaz

2017-04-01

Full Text Available Objective: To determine the role of hepcidin hormone levels in iron accumulation in patients with thalassemia major (TM and thalassemia intermedia (TI. Materials and Methods: Serum prohepcidin and ferritin levels were determined in 34 patient with TM, 10 patient with TI, who attended the Department of Pediatric Hematology Adnan Menderes University Medical Faculty and the Department of Pediatrics at Aydın Atatürk State Hospital between 1 September 2006 and 30 September 2007 and 40 control patients without infection/inflammation, hepatitis or liver failure. Serum prohepcidin levels were measured using a commercial enzyme-linked immunosorbent assay kit (DRG International, Inc. Marburg, Germany; ferritin was studied with chemiluminescence method (Immulite 2000 DPC. Results: Mean serum ferritin levels in TM, TI and control groups were 2347.97±1724.81 ng/mL (range: 144-8015 ng/mL, 1352.40±918.94 ng/mL (range: 311-3109 ng/mL, and 33.35±12.03 ng/mL (range: 20-69.1 ng/mL, respectively. Serum prohepcidin levels in the same groups were 221.78±74.38 ng/mL (range: 7l.14-446.57 ng/mL, 173.31±52.14 ng/mL (range: 100.83-267.69 ng/mL, and 218.20±50.37 ng/mL (range: 116.18-330.43 ng/mL, respectively. There was a statistically significant difference in prohepcidin levels between patients with TI and control group only (p=0.016. No correlation was found between prohepcidin and ferritin levels in all groups (r=-0.023, p=0.839. Conclusion: Low levels of prohepcidin in patients with TI may be related to increased erythropoietic activity. Prohepcidin can be an indicator of active erythropoiesis.

La periferia espontánea en las ciudades intermedias latinoamericanas: perspectivas de solución desde la dimensión territorial-ambiental de la sostenibilidad

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José Jorge Peralta Arias

2017-05-01

Full Text Available La investigación aborda el problema de la insostenibilidad de la periferia espontánea en las ciudades intermedias latinoamericanas, cuya informalidad ha determinado un crecimiento discontinuo, una baja densidad y el déficit de infraestructuras y dotaciones; a esto, se suman los riesgos futuros por desastres de origen natural asociados al cambio climático. En dicho contexto, el trabajo se plantea como objetivo central contribuir a solucionar este problema, a partir de una propuesta metodológica para el estudio de las periferias espontáneas de las ciudades latinoamericanas de escala intermedia, desde la perspectiva del desarrollo sostenible, poniendo énfasis en la dimensión física, ambiental y climática. Para ello, la investigación se organiza en tres partes: revisión y análisis de la información existente sobre la temática y consulta a expertos, para la determinación de criterios; la determinación de acciones prioritarias; y una propuesta de estructura que sustenta un sistema de indicadores. Los resultados del trabajo proponen una batería de treinta y nueve (39 indicadores referenciales, articulados en cuatro subsistemas relacionados con: la vulnerabilidad física-ambiental y climática, el eje ambiental, el desarrollo urbano y la cohesión social. Estos constituyen la principal conclusión del trabajo, el que, se espera, será útil para comenzar a tomar las primeras decisiones frente al problema de este tipo de periferias.

Classical monsters in new Doctor Who fan fiction

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Amanda Potter

2016-03-01

Full Text Available Although a number of classic Doctor Who episodes featured story lines and characters drawn from Greek myth, no new Who episodes based on Greek myth appeared until seasons 5 and 6, in 2010 and 2011. These episodes featured Pandora's box, the Minotaur, and a Siren. They all use the mythical monster or artifact outside of its ancient Greek context, and I argue that the mythical monsters were additions to earlier story ideas. I compare this with the treatment of the myths of the Minotaur and the Sirens in five stories posted to FanFiction.net between 2008 and 2013. These stories all engage with classical myths, and the longest, "Lure of the Sirens," even engages with different versions of the myth of the Sirens. In this article I discuss how the writers use the classical myths within their stories, and how the myths fit in with the primary aims of the writers, for example in developing romantic relationships between characters.

Relationship of the Interaction Between Two Quantitative Trait Loci with γ-Globin Expression in β-Thalassemia Intermedia Patients.

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NickAria, Shiva; Haghpanah, Sezaneh; Ramzi, Mani; Karimi, Mehran

2018-05-10

Globin switching is a significant factor on blood hemoglobin (Hb) level but its molecular mechanisms have not yet been identified, however, several quantitative trait loci (QTL) and polymorphisms involved regions on chromosomes 2p, 6q, 8q and X account for variation in the γ-globin expression level. We studied the effect of interaction between a region on intron six of the TOX gene, chromosome 8q (chr8q) and XmnI locus on the γ-globin promoter, chr11p on γ-globin expression in 150 β-thalassemia intermedia (β-TI) patients, evaluated by statistical interaction analysis. Our results showed a significant interaction between one QTL on intron six of the TOX gene (rs9693712) and XmnI locus that effect γ-globin expression. Interchromosomal interaction mediates through transcriptional machanisms to preserve true genome architectural features, chromosomes localization and DNA bending. This interaction can be a part of the unknown molecular mechanism of globin switching and regulation of gene expression.

A presacral mass in a patient with thalassemia intermedia: A case report and review of the literature

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Mohammad Hossein Anbardar

2016-01-01

Full Text Available Extramedullary hematopoiesis (EMH is defined as the production of the myeloid and erythroid elements outside the bone marrow. It is usually a compensatory mechanism of the myeloid and erythroid production due to increased breakdown or diminished production of erythrocytes. Presacral EMH is an extremely rare condition, and there is a limited number of case reports published in the literature. A 39-year-old female patient as a known case of thalassemia intermedia presented with lower abdominal pain. A computerized tomography scan showed a large presacral mass, associated with bone destruction. The patient was admitted for exploratory laparotomy with suspicion to malignant lesions, but the final pathological diagnosis was EMH. It should be considered in differential diagnosis of mass-like lesions in the presacral area in patients with predisposing factors such as thalassemia, although there were malignant features such as bone destruction in imaging studies. Preoperative diagnostic tools such as fine needle aspiration and biopsy could help us to render the definite diagnosis and prevent unnecessary operation.

El patrimonio modesto de las ciudades intermedias bonaerenses: prácticas usuarias y reflexiones hacia la preservación de las casas "chorizo" de Tandil

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Lorena Marina Sánchez

2014-12-01

Por ello, la indagación se enfoca en el conocimiento del estado de concientización de los usuarios de estas viviendas en la ciudad intermedia de Tandil, cuyo pasado ha forjado un destacado corpus patrimonial modesto que subsiste dentro de la actual dinámica urbana. Se trabaja desde una concepción de la investigación principalmente cualitativa, con ayuda de datos cuantitativos, centrada en la variable histórica urbana-arquitectónica, la social y sus relaciones. Los resultados generales alcanzados, que hacen hincapié en las modificaciones de las viviendas, han posibilitado reflexiones orientadas hacia su salvaguarda.

Una breve descripción de la vegetación, con especial énfasis en las pioneras intermedias de los bosques secos de la Jagua, en la cuenca alta del Rio Magdalena en el Huila

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William Gerardo Vargas

2015-01-01

Full Text Available Se revisó la información existente sobre la flora de los bosques secos del sur del departamento del Huila, con especial interés en la de La Jagua y sus alrededores con el fin de identificar especies pioneras intermedias que puedan apoyar procesos de restauración ecológica y conservación de la biodiversidad. Para los bosques secos del departamento se identificaron 203 especies pioneras intermedias distribuidas en 123 géneros y 45 familias botánicas, mientras que para La Jagua 153 especies agrupadas en 109 géneros y 42 familias, entre las que sobresalen Fabaceae,  Rubiaceae, Malvaceae, Moraceae y Anacardiaceae. Se proponen 85 especies consideradas claves para procesos de conservación, la restauración de los ecosistemas secos de la región dirigida a la generación de hábitat y de conectividad, la producción de recursos para la fauna, así como la producción de leña y madera para las comunidades dentro de un modelo de herramientas de manejo del paisaje.

Identification of Stria Medullaris Fibers in the Massa Intermedia Using Diffusion Tensor Imaging.

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Kochanski, Ryan B; Dawe, Robert; Kocak, Mehmet; Sani, Sepehr

2018-04-01

The massa intermedia (MI) or interthalamic adhesion is an inconsistent band spanning between bilateral medial thalami that is absent in up to 20%-30% of individuals. Little is known of its significance, especially in regard to functional pathways. Probabilistic diffusion tensor imaging (DTI) has recently been used to seed the lateral habenula and define its afferent white matter pathway, the stria medullaris thalami (SM). We sought to determine whether the MI serves as a conduit for crossing of limbic fibers such as the SM. Probabilistic DTI was performed on 10 subjects who had presence of a MI as visualized on magnetic resonance imaging. Tractography was also performed on 2 subjects without MI. Manual identification of the lateral habenula on axial T1-weighted magnetic resonance imaging was used for the initial seed region for tractography. In all subjects, the SM was reliably visualized. In 7 of the 10 subjects with MI, there was evidence of SM fibers that crossed to the ipsilateral hemisphere. Three subjects with small diameter MI did not have tractographic evidence of crossing SM fibers. Of the 7 subjects with crossing SM fibers within the MI, 5 showed predilection toward the right orbitofrontal cortex from both the left and right seed regions. Probabilistic DTI provides evidence of SM fibers within the MI. Given its anatomic location as a bridging pathway between thalami, further studies are necessary to assess its role within the limbic functional network. Copyright © 2018 Elsevier Inc. All rights reserved.

Beyond the myth: The mermaid syndrome from Homerus to Andersen

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Romano, Stefania; Esposito, Vincenzo; Fonda, Claudio; Russo, Anna; Grassi, Roberto

2006-01-01

Mermaid or sirens have been part of the cultural tradition of the sailors during the first expeditions in the western world. The Siren's Myth appeared for a first time with Homer, who described in the Odyssey some singing creatures that lured the enchanted sailors to death. More frequently described with a bird body and a female head, sometimes the female part was extended to torso, with arms prolonged in sturdy claws. In the Latin literature Publius Ovidius Naso presented in the Metamorphoses these creatures. Proposed ethimology for the word 'siren' seems to confirm the prerogatives of these creatures, related to magnetism, seduction, charm. The first figuration of Sirens resembling to fish-women was in the second century BC. Hans Christian Andersen provided to leave us the strongest legend of Siren in the well-known fairy tale 'The Little Mermaid'. Following this story, Sirens are definitely considered as beautiful half-fish women who lived in the bottom of the sea, having a lovely voice to be used when they rise up to allow sweeter the agony of the wrecked sailors. Beyond the Myth, may the Siren really exist? It can be hypothesized that these creatures probably were individuals affected by sirenomelia. In our literature and medical review, we describe the etiology of the disease, and we illustrated the anatomical features of fetuses affected by this pathology using MDCT 3D reconstructions. Syrenomelia is a condition not compatible with the normal life, however nine cases of 'mermaid' survived to reconstructive surgery have been reported until now. In our report we also presented a case of survival baby girl affected by sirenomelia, before and after surgery, with correlative radiologic imaging findings. The most important characteristic that seems to allow survival of the affected individuals is the presence of one functional kidney, displaced in pelvis. As so dramatically tragic was the history of the Andersen Little Mermaid, so unattended pleasant would be the

Beyond the myth: The mermaid syndrome from Homerus to Andersen

Energy Technology Data Exchange (ETDEWEB)

Romano, Stefania [Department of Diagnostic Imaging, ' A. Cardarelli' Hospital, Viale Cardarelli 9, 80131 Naples (Italy)]. E-mail: stefromano@libero.it; Esposito, Vincenzo [Institute of Anatomy, Second University of Naples, Via L. Armanni 5, 80138 Naples (Italy); Fonda, Claudio [Pediatric Radiology Section, Meyer' s Children Hospital, Florence (Italy); Russo, Anna [Institute of Anatomy, Second University of Naples, Via L. Armanni 5, 80138 Naples (Italy); Grassi, Roberto [Institute of Radiology, Second University of Naples, Via L. Armanni 5, 80138 Naples (Italy)

2006-05-15

Mermaid or sirens have been part of the cultural tradition of the sailors during the first expeditions in the western world. The Siren's Myth appeared for a first time with Homer, who described in the Odyssey some singing creatures that lured the enchanted sailors to death. More frequently described with a bird body and a female head, sometimes the female part was extended to torso, with arms prolonged in sturdy claws. In the Latin literature Publius Ovidius Naso presented in the Metamorphoses these creatures. Proposed ethimology for the word 'siren' seems to confirm the prerogatives of these creatures, related to magnetism, seduction, charm. The first figuration of Sirens resembling to fish-women was in the second century BC. Hans Christian Andersen provided to leave us the strongest legend of Siren in the well-known fairy tale 'The Little Mermaid'. Following this story, Sirens are definitely considered as beautiful half-fish women who lived in the bottom of the sea, having a lovely voice to be used when they rise up to allow sweeter the agony of the wrecked sailors. Beyond the Myth, may the Siren really exist? It can be hypothesized that these creatures probably were individuals affected by sirenomelia. In our literature and medical review, we describe the etiology of the disease, and we illustrated the anatomical features of fetuses affected by this pathology using MDCT 3D reconstructions. Syrenomelia is a condition not compatible with the normal life, however nine cases of 'mermaid' survived to reconstructive surgery have been reported until now. In our report we also presented a case of survival baby girl affected by sirenomelia, before and after surgery, with correlative radiologic imaging findings. The most important characteristic that seems to allow survival of the affected individuals is the presence of one functional kidney, displaced in pelvis. As so dramatically tragic was the history of the Andersen Little Mermaid

La ordenación territorial en el País Vasco. Debilidades, amenazas,fortalezas y oportunidades de la escala intermedia

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Pedro J. Lozano Valencia

2003-01-01

Full Text Available La ordenación del territorio encuentra su razón de ser, en el País Vasco, a través de las Directrices de Ordenación y dos tipos diferentes de documentos: los Planes Territoriales Parciales y los Planes Territoriales Sectoriales. Una vez delimitadas las comarcas y los sectores estratégicos, se aplican dichos documentos. Con todo, se logra la coordinación de las Directivas europeas a escala superior, y los Planes y Normas Subsidiarias de Ordenación Urbana a una escala inferior o local. En medio quedan todas esas herramientas de ordenación mencionadas anteriormente, que conforman lo que se ha venido a definir como la escala intermedia. Ésta aparece como un marco muy apropiado para abordar las debilidades, amenazas, fortalezas y oportunidades que, en un horizonte de 16 a 20 años, presenta el territorio vasco

Determination of Five Chemical Markers in DF Formula, the Herbal Composition of Ephedra intermedia, Rheum palmatum, and Lithospermum erythrorhizon, Using High-performance Liquid Chromatography-ultraviolet Detection

Science.gov (United States)

Jeong, Birang; Roh, Jong Seong; Yoon, Michung; Yoon, Yoosik; Shin, Soon Shik; Cho, Hyun Joo; Kwon, Yong Soo; Yang, Heejung

2018-01-01

Background: DF formula is a herbal preparation comprised three medicinal herbs, namely, Ephedra intermedia, Rheum palmatum, and Lithospermum erythrorhizon, which is being used for the treatment of obesity and liver fibrosis in Korean local clinics. Objective: Since the abovementioned three herbs exist with different proportions in DF formula and their chemical markers have different physiochemical properties; it is quite challenging to develop an analytical methodology for the determination of these chemical markers. Materials and Methods: For the analysis of the three herbs, five chemicals, (+)-pseudoephedrine (1) and (−)-ephedrine (2) for E. intermedia, aloe-emodin (3), and chrysophanol (4) for R. palmatum, and shikonin (5) for L. erythrorhizon, were selected for method validation of DF formula, and the analytical conditions were optimized and validated using high-performance liquid chromatography coupled with an ultraviolet detector (HPLC-UV). Results: The specificities for the five compounds 1–5 were determined by their UV absorption spectra (1–4: 215 nm and 5: 520 nm). Their calibration curves showed good linear regressions with high correlation coefficient values (R2 > 0.9997). The limits of detection of these five markers were in the range 0.4–2.1 ng/mL, with the exception of 5 (12.7 ng/mL). The intraday variability for all the chemical markers was less than a Relative standard deviation (RSD) of 3%, except for 5 (RSD = 12.6%). In the case of interday analysis, 1 (1.0%), 2 (3.1%), and 4 (3.7%) showed much lower variabilities (RSD High-performance liquid chromatography; UV: Ultraviolet; LOD: Limit of detection; LOQ: Limit of quantification; RSD: Relative standard deviation. PMID:29720825

HOMER Economic Models - US Navy

Energy Technology Data Exchange (ETDEWEB)

Bush, Jason William [Idaho National Lab. (INL), Idaho Falls, ID (United States); Myers, Kurt Steven [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2016-02-01

This LETTER REPORT has been prepared by Idaho National Laboratory for US Navy NAVFAC EXWC to support in testing pre-commercial SIREN (Simulated Integration of Renewable Energy Networks) computer software models. In the logistics mode SIREN software simulates the combination of renewable power sources (solar arrays, wind turbines, and energy storage systems) in supplying an electrical demand. NAVFAC EXWC will create SIREN software logistics models of existing or planned renewable energy projects at five Navy locations (San Nicolas Island, AUTEC, New London, & China Lake), and INL will deliver additional HOMER computer models for comparative analysis. In the transient mode SIREN simulates the short time-scale variation of electrical parameters when a power outage or other destabilizing event occurs. In the HOMER model, a variety of inputs are entered such as location coordinates, Generators, PV arrays, Wind Turbines, Batteries, Converters, Grid costs/usage, Solar resources, Wind resources, Temperatures, Fuels, and Electric Loads. HOMER's optimization and sensitivity analysis algorithms then evaluate the economic and technical feasibility of these technology options and account for variations in technology costs, electric load, and energy resource availability. The Navy can then use HOMER’s optimization and sensitivity results to compare to those of the SIREN model. The U.S. Department of Energy (DOE) Idaho National Laboratory (INL) possesses unique expertise and experience in the software, hardware, and systems design for the integration of renewable energy into the electrical grid. NAVFAC EXWC will draw upon this expertise to complete mission requirements.

Regression of extramedullary hematopoiesis with hydroxyurea therapy in ß-thalassemia intermedia Regressão da hematopoese extramedular na talassemia intermédia após terapia com hidroxiuréia

Directory of Open Access Journals (Sweden)

Perla Vicari

2006-03-01

Full Text Available Excessive ineffective erythropoiesis in thalassemia intermedia may cause extramedullary hematopoiesis (EMH, resulting in spleen and liver enlargement or masses in several tissues, mainly paravertebrally. Other less frequent locations of diffuse compensatory EMH are kidneys, adrenal glands, breasts, spinal cord, pleura, pericardium, duramater, adipose tissue and skin, although intrathoracic extramedullary hematopoiesis is a rare condition. Management strategies have included radiation and transfusion therapy. Hydroxyurea with transfusion therapy has been associated with clinical regression of EMH in thalassemia. We report an uncommon case of intrathoracic EMH in a patient with beta-thalassemia intermedia, that showed significant recovery with HU therapy.A excessiva eritropoese ineficaz na talassemia pode causar hemato-poese extramedular (HEM, resultando em hepatomegalia, esplenomegalia e massas de tecido hematopoético em diversos tecidos. Localizações de HEM compensatória menos freqüentes são rins, glândulas adrenais, canal medular, pleura, pericárdio, duramáter, tecido adiposo e pele. Entretanto, HEM intratorácica é condição rara. Estratégias terapêuticas incluem radiação e transfusões sanguíneas. O uso de hidroxiuréia concomitante a terapêutica transfusional foi associado à regressão clínica da HEM na talassemia. Nós descrevemos um caso de HEM intratorácica em paciente portadora de talassemia intermédia, com significante regressão do quadro após terapêutica isolada com hidroxiuréia.

Jóvenes sin vínculos. El papel de las estructuras intermedias en un espacio urbano desfavorecido

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Cecilia Eseverri Mayer

2015-01-01

Full Text Available Este artículo describe el proceso de desafiliación (Castel, 1995 juvenil, tomando como caso de estudio un barrio desfavorecido de Madrid. A través de una metodología etnográfica examina el efecto de las estructuras intermedias (la familia, la comunidad local y las asociaciones civiles en la reinserción educativa y laboral de los jóvenes de origen marroquí, dominicano y ecuatoriano. La investigación señala como elemento más determinante de dicha reinserción el capital humano de los padres, confi rmando los hallazgos de la sociología de la educación (Bourdieu, 1970. Además, advierte del doble efecto (positivo y negativo que provoca la influencia de las comunidades étnicas en los jóvenes más vulnerables y demuestra el rol crucial que cumple el tejido asociativo. Estas organizaciones, herederas de las movilizaciones de los años 70, tienen la capacidad de abrir nuevas vías de inserción a través de la colaboración con las administraciones, los grupos étnicos y las familias.

Information and interaction with the public

International Nuclear Information System (INIS)

Grlicarev, I.

2003-01-01

before the Operation of the facility. The Slovenian Protection against Natural and Other Disasters Act (PNODA) stipulates the notification to the affected population in case of an emergency only. Distributing prior information to the population likely to be affected is not mandatory. Based an the provisions of the PNODA the system for dissemination of information to the affected population has been established. The notification centres serve as the communication nodes which collect the initial information and distribute it according to pre-defined scheine to local or state authorities or other organisations. For alerting the population the siren system can be triggered from the notification centre. If the sirens are activated the population should go indoors and listen to the advice given by the selected radio stations and TV channels. The nuclear emergency plans are devised and maintained by the civil protection authorities. The regulation on emergency planning requires organisation of a public hearing before the emergency plans are adopted. Although the prior information to the population is not enforced by law, the Krsko NPP issued and distributed brochure to the population in the urgent protective actions planning zone. In Slovenia the urgent protective actions planning zone is the area within 10 km around the Krsko NPP. For the information flow in case of a nuclear or radiological emergency the following organizations are the most important: the National Civil Protection Headquarters together with the Regional Civil Protection Headquarters for potentially affected population; the Governmental Office for Information for general type of news for the rest of (non affected) population. The news are conveyed to the news agencies in form of press releases or press conferences by the Governmental Office for Information, which receives support in preparation of press releases from the National Civil Protection Headquarters or directly from the SNSA, if the National Civil

Reconstructing the dark sector interaction with LISA

Energy Technology Data Exchange (ETDEWEB)

Cai, Rong-Gen; Yang, Tao [CAS Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Sciences, P.O. Box 2735, Beijing 100190 (China); Tamanini, Nicola, E-mail: cairg@itp.ac.cn, E-mail: nicola.tamanini@cea.fr, E-mail: yangtao@itp.ac.cn [Institut de Physique Théorique, CEA-Saclay, CNRS UMR 3681, Université Paris-Saclay, F-91191 Gif-sur-Yvette (France)

2017-05-01

We perform a forecast analysis of the ability of the LISA space-based interferometer to reconstruct the dark sector interaction using gravitational wave standard sirens at high redshift. We employ Gaussian process methods to reconstruct the distance-redshift relation in a model independent way. We adopt simulated catalogues of standard sirens given by merging massive black hole binaries visible by LISA, with an electromagnetic counterpart detectable by future telescopes. The catalogues are based on three different astrophysical scenarios for the evolution of massive black hole mergers based on the semi-analytic model of E. Barausse, Mon. Not. Roy. Astron. Soc. 423 (2012) 2533. We first use these standard siren datasets to assess the potential of LISA in reconstructing a possible interaction between vacuum dark energy and dark matter. Then we combine the LISA cosmological data with supernovae data simulated for the Dark Energy Survey. We consider two scenarios distinguished by the time duration of the LISA mission: 5 and 10 years. Using only LISA standard siren data, the dark sector interaction can be well reconstructed from redshift z ∼1 to z ∼3 (for a 5 years mission) and z ∼1 up to z ∼5 (for a 10 years mission), though the reconstruction is inefficient at lower redshift. When combined with the DES datasets, the interaction is well reconstructed in the whole redshift region from 0 z ∼ to z ∼3 (5 yr) and z ∼0 to z ∼5 (10 yr), respectively. Massive black hole binary standard sirens can thus be used to constrain the dark sector interaction at redshift ranges not reachable by usual supernovae datasets which probe only the z ∼< 1.5 range. Gravitational wave standard sirens will not only constitute a complementary and alternative way, with respect to familiar electromagnetic observations, to probe the cosmic expansion, but will also provide new tests to constrain possible deviations from the standard ΛCDM dynamics, especially at high redshift.

Molecular Basis of β-Thalassemia Intermedia in Erbil Province of Iraqi Kurdistan.

Science.gov (United States)

Shamoon, Rawand P; Al-Allawi, Nasir A S; Cappellini, Maria D; Di Pierro, Elena; Brancaleoni, Valentina; Granata, Francesca

2015-01-01

β-Thalassemia intermedia (β-TI) is a clinical term describing a range of clinical phenotypes that are intermediate in severity between the carrier state and β-thalassemia major (β-TM). To characterize the molecular basis of β-TI in Erbil Province, Northern Iraq, 83 unrelated patients were investigated. Detection of β-globin gene mutations was carried out by reverse hybridization assay and direct gene sequencing. All patients were screened for the XmnI polymorphism by direct sequencing of HBG2 ((G)γ promoter gene). Detection of α-globin gene deletions and triplication was carried out using the reverse hybridization assay. Four main molecular patterns were identified in association with the β-TI phenotype, namely: β(+)/β(+) (38.5%), β(+)/β(0) (21.6%), β(0)/β(0) (31.3%), and β(0)/wild type (8.4%). IVS-I-6 (T > C) was the most frequently encountered mutation (55 alleles, 34.6%), followed by IVS-II-1 (G > A) and codon 8 (-AA); furthermore, we report for the first time from Iraq two β(+) mutations, -87 (C > G) and 5' untranslated region (5'UTR) +22 (G > A). The XmnI polymorphism was detected in 47.0% of patients, mainly in association with the β(0)/β(0) genotype. The α-globin gene deletions were encountered in four cases, including one case with (- -(FIL)) double gene deletion, a report that is the first from our country. The α-globin gene triplication was detected in five of the seven heterozygous β-thalassemia (β-thal) patients. Similar to other Mediterranean countries, inheritance of mild β-globin mutations was the main molecular pattern underlying β-TI in our patients followed by the ameliorating effect of the XmnI polymorphism.

Flood warnings in coastal areas: how do experience and information influence responses to alert services?

Science.gov (United States)

Pescaroli, G.; Magni, M.

2015-04-01

Many studies discuss the economic and technical aspects of flood warnings. Less attention has been given to the social and behavioural patterns that affect alert services. In particular, the literature focuses on warnings activated in river basins or marine environments without providing clear evidence on Mediterranean coastal areas, even though these are subjected to growing flood risk related to climate change. This paper is a first attempt to bridge this gap. Our research develops an in-depth analysis of the village of Cesenatico on the Adriatic Sea coast. Here the municipality adopted two complementary warning systems: a siren and an alert via short message service (SMS). The analysis focuses on a survey conducted in 2011 and 2012 with 228 participants. The relationships between social and behavioural variables and warning services are investigated as well as flood preparedness and information dissemination. Qualitative evidence from informal interviews is used to support the understanding of key responses. The conclusions show how different social and behavioural patterns can influence the effectiveness and use of warning systems, regardless of the technology adopted and the structural mitigation measures implemented. Education, training and accountability are seen to be critical elements for implementation. Finally, the statistical output is used to suggest new questions and new directions for research.

Evaluation of the prompt alerting systems at four nuclear power stations

International Nuclear Information System (INIS)

Towers, D.A.; Anderson, G.S.; Keast, D.N.; Kenoyer, J.L.; Desrosiers, A.E.

1982-09-01

This report presents evaluations of the prompt notification siren systems at the following four US nuclear power facilities: Trojan, Three Mile Island, Indian Point, and Zion. The objective of these evaluations was to provide examples of an analytical procedure for predicting siren-system effectiveness under specific conditions in the 10-mile emergency planning zone (EPZ) surrounding nuclear power plants. This analytical procedure is discussed in report No. PNL-4227

Thalassemia intermedia as a result of heterozygosis for ß0-thalassemia and aaaanti-3.7/aa genotype in a Brazilian patient

Directory of Open Access Journals (Sweden)

Kimura E.M.

2003-01-01

Full Text Available We report a case in which the interaction of heterozygosis for both the ß0-IVS-II-1 (G->A mutation and the aaaanti-3.7 allele was the probable cause for the clinical occurrence of thalassemia intermedia. The propositus, a 6-year-old Caucasian Brazilian boy of Portuguese descent, showed a moderately severe chronic anemia in spite of having the ß-thalassemia trait. Investigation of the alpha-globin gene status revealed heterozygosis for alpha-gene triplication (aaa/aa. The patient's father, also presenting mild microcytic and hypochromic anemia, had the same alpha and ß genotypes as his son, while the mother, not related to the father and hematologically normal, was also a carrier of the aaaanti-3.7 allele. The present case emphasizes the need for considering the possibility of alpha-gene triplication in ß-thalassemia heterozygotes who display an unexpected severe phenotype. The ß-thalassemia mutation found here is being described for the first time in Brazil.

An Extraordinary Accumulation of (-)-Pinoresinol in Cell-Free Extracts of Forsythia intermedia: Evidence for Enantiospecific Reduction of (+)-Pinoresinol

Science.gov (United States)

Katayama, Takeshi; Davin, Laurence B.; Lewis, Norman G.

1992-01-01

Stereoselective and enantiospecific transformation mechanisms in lignan biogenesis are only now yielding to scientific inquiry: it has been shown that soluble cell-free preparations from Forsythia intermedia catalysis the formation of the enantiomerically pure lignan, (-)-secoisolariciresinol, when incubated with coniferyl alcohol in the presence of NAD(P)H and H2O2. Surprisingly, (-)-pinoresinol also accumulates in this soluble cell-free assay mixture in greater than 96% enantiomeric excess, even though it is not the naturally occurring antipode present in Forsythia sp. But these soluble cell-free preparations do not engender stereoselective coupling; instead, racemic pinoresinols are first formed, catalysed by an H2O2-dependent peroxidase reaction. An enantiospecific NAD(P)H reductase then converts (+)- pinoresinol, and not the (-)-antipode, into (-)-secoisolariciresinol. Stereoselective syntheis of(+)-pinoresinol from E-coniferyl alcohol is, however, catalysed by an insoluble enzyme preparation in F. suspensa, obtained following removal of readily soluble and ionically bound enzymes; no exogenously supplied cofactors were required other than oxygen, although the reaction was stimulated by NAD-malate addition. Thus, the overall biochemical pathway to enantiomerically pure (-)-secoisolariciresinol has been delineated.

Rat beta-LPH, gamma-LPH and beta-endorphin biosynthesized by isolated cells of pars intermedia and pars distalis

International Nuclear Information System (INIS)

Gianoulakis, C.; Seidah, N.G.; Routhier, R.; Chretien, M.

1980-01-01

Rats pars intermedia cells were incubated for 3 h with the following amino-acids: a) 35 S-methionine and 3 H-phenylalamine; b) 3 H-valine; and c) 3 H-valine and 3 H-lysine. Radioactive gamma-lipotropin, beta-lipotropin and beta-endorphin were purified on carboxy- methyl-cellulose and characterized by polyacrylamide disc gel electrophoresis af pH 4.5, molecular weight estimation and microsequencing. Rat gamma-lipotropin was shown to differ slightly from ovine gamma-lipotropin in its NH 2 -terminal amino acid sequence, in containing no methionine and having phenylalanine at position 6, valine at positions 13 and 27, and lysine at position 20. The same variations were observed in the sequence of rat beta-lipotropin, while rat beta-endorphin was shown to be identical to the ovine beta-endorphin. Following a 3-h pulse of rat pars distalis, the cells were extracted with care to avoid beta-lipotropin degradation by proteolytic enzymes. A peptide was purified and identified to be rat beta-endorphin, thus demonstrating that beta-endorphin is biosynthesized in pars distalis and is not an extraction artifact. (author)

Los elementos traza (Mg, Sr, Ha, Fe, Mn) en carbonatos: ambiente genético del Karst del techo de la unidad intermedia de la Cuenca neógena de Madrid.

OpenAIRE

Cañaveras Jiménez, Juan Carlos; Ordóñez Delgado, Salvador; Hoyos Gómez, Manuel; Calvo Sorando, José Pedro

1992-01-01

Este trabajo trata de caracterizar geoquímicamente el karst del techo de la U. Intermedia del Mioceno en las partes centrales de la Cuenca de Madrid. Para ello se han estudiado una serie de elementos traza (Mg, Sr, Na, Fe y Mn) mediante fluorescencia de rayos X. En base a este estudio se han distinguido dos tipos de procesos: De reemplazamiento y/o recristalización, que implican la litificación y homogeneización química de un sedimento calcítico-dolomítico; y procesos de precipitación química...

Cómo se negocia una posición de sujeto intermedia: muchachos entre once y catorce años, masculinidades y educación escolar

Directory of Open Access Journals (Sweden)

Ann Phoenix

2002-04-01

Full Text Available Este ensayo pone a discusión la ciencia (generalizada en las escuelas de Londres según la cual existe una oposición entre la masculinidad y la realización de tareas escolares, que tiene fuertes efectos en la subjetividad de lo masculino del joven. Así considera las formas en que éstos asisten a las escuelas de Londres y negocian lo que ellos consideran las contradicciones que involucra el tratar de reconciliar el hacer las tareas escolares con ser aceptado por los otros en su condición masculina. El documento se centra particularmente en la forma como los muchachos negocian una posición de sujeto intermedia, en un intento de asegurarse que trabajaron lo suficiente para no reprobar, pero no tanto como para llamar la atención de los otros, por no ser “propiamente masculinos”. El texto también afirma que estas contradicciones tienen una carga emocional, de modo que los jóvenes se esfuerzan en negociar lo que ellos consideran una posición intermedia, pues esta es la posición de sujeto que les resulta menos problemática ocupar.This paper discusses how the construction (pervasive in London schools that masculinities and doing schoolwork are antithetical to each other has an impact on boys’ subject positions. It considers the ways in which boys attending schools in London negotiate what they see as the contradictions involved in attempting to reconcile doing schoolwork with being accepted by other boys as masculine.In particular, it focuses on how they negotiate a middle subject position, attempting to ensure that they did sufficient work not to fail at school, but not so much that they came to other boys’ attention as not ‘properly masculine’. The paper argues that these contradictions are emotionally charged, so that boys put effort into negotiating what they see as a middle position because this is the least problematic subject position for them to take up.

TECHNIQUES DE MYSTIFICATION DU SACRÉ DANS LA LITTÉRATURE MYTHOLOGISANTE DE LA GRÈCE ANTIQUE

OpenAIRE

Dr. Maria-Luiza Oancea (Dumitru)

2008-01-01

This paper entails the sacred mytification motif, a subject hardly debated, but extremely challenging, that we can identify in several myths and episodes of the ancient Greek mythology literature (Thanatos-Sisyphus myth, Sirens-Odysseus or Sirens-Orpheus myth, Polifem-Odysseus myth, Gorgo Medusa-Perseus myth, etc.). Our essay starts from the general thesis that a direct confrontation with the Sacred is essentially impossible, making the Sacred almost always manipulated (cf. R. Caillois, R. Gi...

Environmental Assessment of the Construction of the Joint Use Small Arms Range for the 934th Airlift Wing at Minneapolis-St. paul International Airport Air Reserve Station, Minnesota

Science.gov (United States)

2006-10-01

environments can also mask verbal or mechanical warning signals such as sirens, bells, or horns. Range safety violations and unsafe operating conditions must...January 29, 1993), CEQ provides guidance to Federal agencies on how to “incorporate pollution prevention principles, techniques, and mechanisms into...take no formal action on the EA. If you have any questions or need further information, please contact Denise Pedersen Engen , principal reviewer for

Steps towards the institutionalisation of feminism in Norway

Directory of Open Access Journals (Sweden)

Alexandra COLUMBAN

2011-01-01

Full Text Available The present article discusses important issues related to the institutionalisation of feminism in Norway, by providing a case study on the country’s first feminist magazine, Sirene. The first part of the article focuses on the political and historical context which favoured the emergence of the magazine, and insists on a series of legal measures taken in the twentieth century – suffrage rights, equal payment, political representation, etc. – which provided the institutional framework for the women’s movement. The more comprehensive second part of the article focuses on Sirene itself: it analyses the magazine’s structure and key concepts, its political agenda, its aims, but also thebranch of feminism to which Sirene adheres. The article concludes that the magazine represented an influential alternative to the mainstream press of the time, and contributed greatly to achieving the goals of women’s liberation movement.

Anuncios y estrategias de campaña en televisión: un balance de la elección intermedia de 2015 en México

Directory of Open Access Journals (Sweden)

Oniel Francisco Díaz Jiménez

2016-10-01

Full Text Available En las campañas electorales profesionalizadas, los partidos políticos y los candidatos presentan sus propuestas ante el electorado y buscan maximizar votos, principalmente, a través de los medios de comunicación. En elecciones presidenciales, las estrategias y las tácticas de campaña se enfocan en las imágenes y las propuestas políticas de los candidatos que compiten por la Presidencia. Sin embargo, ¿qué ocurre en elecciones congresionales intermedias, donde los actores principales de la campaña son los partidos políticos y sus candidatos que compiten por cargos legislativos? Este trabajo realiza un análisis de contenido de los anuncios televisivos (spots de 30 segundos transmitidos por los partidos mexicanos durante las elecciones congresionales intermedias de 2015, con el fin de identificar sus estrategias de campaña. Los resultados muestran diferencias relevantes entre las campañas realizadas por las organizaciones nacionales de los partidos y las campañas de los candidatos individuales a nivel distrital. Sin embargo, también ponen a la vista que los anuncios de ataque fueron un componente central en las estrategias mediáticas de los partidos y los candidatos durante la campaña. / In professionalized election campaigns, political parties and candidates present their policy proposals to the electorate and seek to maximize votes, primarily through media appeals. In presidential elections, campaign tactics and strategies focus on the images and policy proposals of presidential candidates. However, what happens in congressional midterm elections, where the main campaigners are the political parties and their congressional candidates? This article analyzes the content of all television announcements (30-second spots broadcast by Mexican political parties during the 2015 mid-term congressional elections, in order to identify their campaign strategies. The results show relevant differences between the campaigns run by national

Progression of periodontal inflammation in adolescents is associated with increased number of Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, and Fusobacterium nucleatum.

Science.gov (United States)

Yang, Ning-Yan; Zhang, Quan; Li, Jin-Lu; Yang, Sheng-Hui; Shi, Qing

2014-05-01

The study aims to evaluate the change of related subgingival periodontopathogens among different stage of gingivitis in adolescent and assess the relationship between periodontopathogens and the progression of periodontal inflammation. A total of 77 subgingival plaque samples from 35 adolescent individuals were divided into three groups including gingivitis group (mild, 15 samples; moderate, 16 samples; severe, 15 samples), chronic periodontitis group (15 samples) and healthy group (15 samples). Real-time PCR was used to quantitate Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, and Fusobacterium nucleatum in subgingival plaque samples. All species, except for F. nucleatum, were detected in samples from gingivitis and periodontitis groups in significantly greater number than in those from healthy group (P periodontitis group in significantly greater number than in those from moderate-to-severe gingivitis group (P periodontal inflammation in adolescents. Examination of periodontopathogens number in adolescents may be of some value for monitoring of periodontal disease development. © 2013 BSPD, IAPD and John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Association of Distinct Fine Specificities of Anti-Citrullinated Peptide Antibodies With Elevated Immune Responses to Prevotella intermedia in a Subgroup of Patients With Rheumatoid Arthritis and Periodontitis.

Science.gov (United States)

Schwenzer, Anja; Quirke, Anne-Marie; Marzeda, Anna M; Wong, Alicia; Montgomery, Anna B; Sayles, Harlan R; Eick, Sigrun; Gawron, Katarzyna; Chomyszyn-Gajewska, Maria; Łazarz-Bartyzel, Katarzyna; Davis, Simon; Potempa, Jan; Kessler, Benedikt M; Fischer, Roman; Venables, Patrick J; Payne, Jeffrey B; Mikuls, Ted R; Midwood, Kim S

2017-12-01

In addition to the long-established link with smoking, periodontitis (PD) is a risk factor for rheumatoid arthritis (RA). This study was undertaken to elucidate the mechanism by which PD could induce antibodies to citrullinated peptides (ACPAs), by examining the antibody response to a novel citrullinated peptide of cytokeratin 13 (CK-13) identified in gingival crevicular fluid (GCF), and comparing the response to 4 other citrullinated peptides in patients with RA who were well-characterized for PD and smoking. The citrullinomes of GCF and periodontal tissue from patients with PD were mapped by mass spectrometry. ACPAs of CK13 (cCK13), tenascin-C (cTNC5), vimentin (cVIM), α-enolase (CEP-1), and fibrinogen β (cFIBβ) were examined by enzyme-linked immunosorbent assay in patients with RA (n = 287) and patients with osteoarthritis (n = 330), and cross-reactivity was assessed by inhibition assays. A novel citrullinated peptide cCK13-1 ( 444 TSNASGR-Cit-TSDV-Cit-RP 458 ) identified in GCF exhibited elevated antibody responses in RA patients (24%). Anti-cCK13-1 antibody levels correlated with anti-cTNC5 antibody levels, and absorption experiments confirmed this was not due to cross-reactivity. Only anti-cCK13-1 and anti-cTNC5 were associated with antibodies to the periodontal pathogen Prevotella intermedia (P = 0.05 and P = 0.001, respectively), but not with antibodies to Porphyromonas gingivalis arginine gingipains. Levels of antibodies to CEP-1, cFIBβ, and cVIM correlated with each other, and with smoking and shared epitope risk factors in RA. This study identifies 2 groups of ACPA fine specificities associated with different RA risk factors. One is predominantly linked to smoking and shared epitope, and the other links anti-cTNC5 and cCK13-1 to infection with the periodontal pathogen P intermedia. © 2017 The Authors. Arthritis & Rheumatology published by Wiley Periodicals, Inc. on behalf of American College of Rheumatology.

The psychosomatic symptom and the self: a sirens' song.

Science.gov (United States)

Kradin, R L

1997-07-01

This paper examines the symbolic nature of the psychosomatic symptom. It is suggested that the psychosomatic symptom is an informationally rich symbolic derivative of the Self that serves to focus attention on developmental disturbances in the archetypal processes of constructing body image and interpreting dysphoric somatic sensations. Clinical examples are offered to illustrate the changing nature of the psychomatic symptom in society. The therapeutic importance of monitoring affectual transactions in the transference-countertransference field is stressed.

Agenda Trending: Reciprocity and the Predictive Capacity of Social Networking Sites in Intermedia Agenda Setting across Topics over Time

Directory of Open Access Journals (Sweden)

Jacob Groshek

2013-08-01

Full Text Available In the contemporary converged media environment, agenda setting is being transformed by the dramatic growth of audiences that are simultaneously media users and producers. The study reported here addresses related gaps in the literature by first comparing the topical agendas of two leading traditional media outlets (New York Times and CNN with the most frequently shared stories and trending topics on two widely popular Social Networking Sites (Facebook and Twitter. Time-series analyses of the most prominent topics identify the extent to which traditional media sets the agenda for social media as well as reciprocal agenda-setting effects of social media topics entering traditional media agendas. In addition, this study examines social intermedia agenda setting topically and across time within social networking sites, and in so doing, adds a vital understanding of where traditional media, online uses, and social media content intersect around instances of focusing events, particularly elections. Findings identify core differences between certain traditional and social media agendas, but also within social media agendas that extend from uses examined here. Additional results further suggest important topical and event-oriented limitations upon the predictive capacit of social networking sites to shape traditional media agendas over time.

Predicting Ambulance Time of Arrival to the Emergency Department Using Global Positioning System and Google Maps

Science.gov (United States)

Fleischman, Ross J.; Lundquist, Mark; Jui, Jonathan; Newgard, Craig D.; Warden, Craig

2014-01-01

Objective To derive and validate a model that accurately predicts ambulance arrival time that could be implemented as a Google Maps web application. Methods This was a retrospective study of all scene transports in Multnomah County, Oregon, from January 1 through December 31, 2008. Scene and destination hospital addresses were converted to coordinates. ArcGIS Network Analyst was used to estimate transport times based on street network speed limits. We then created a linear regression model to improve the accuracy of these street network estimates using weather, patient characteristics, use of lights and sirens, daylight, and rush-hour intervals. The model was derived from a 50% sample and validated on the remainder. Significance of the covariates was determined by p times recorded by computer-aided dispatch. We then built a Google Maps-based web application to demonstrate application in real-world EMS operations. Results There were 48,308 included transports. Street network estimates of transport time were accurate within 5 minutes of actual transport time less than 16% of the time. Actual transport times were longer during daylight and rush-hour intervals and shorter with use of lights and sirens. Age under 18 years, gender, wet weather, and trauma system entry were not significant predictors of transport time. Our model predicted arrival time within 5 minutes 73% of the time. For lights and sirens transports, accuracy was within 5 minutes 77% of the time. Accuracy was identical in the validation dataset. Lights and sirens saved an average of 3.1 minutes for transports under 8.8 minutes, and 5.3 minutes for longer transports. Conclusions An estimate of transport time based only on a street network significantly underestimated transport times. A simple model incorporating few variables can predict ambulance time of arrival to the emergency department with good accuracy. This model could be linked to global positioning system data and an automated Google Maps web

Biodegradation of diuron by an endophytic fungus Neurospora intermedia DP8-1 isolated from sugarcane and its potential for remediating diuron-contaminated soils.

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Wang, Yanhui; Li, Honghong; Feng, Guojun; Du, Liangwei; Zeng, Dongqiang

2017-01-01

A diuron-degrading endophyte DP8-1 was isolated from sugarcane root grown in diuron-treated soil in the present study. The endophyte was identified as Neurospora intermedia based on the morphological characteristics and sequence analysis. The fermentation parameters including temperature, pH, inoculation size, carbon source, and initial diuron concentration were also investigated for the optimization of degradation efficiency. The results indicated that strain DP8-1 was capable of degrading up to 99% diuron within 3 days under the optimal degrading condition. The study of degradation spectrum indicated that strain DP8-1 could also degrade and utilize fenuron, monuron, metobromuron, isoproturon, chlorbromuron, linuron, and chlortoluron as substrate for strain growth. On basis of liquid chromatography-mass spectrometry analysis for the products of the degradation of diuron, strain DP8-1 metabolized diuron to produce N-(3,4-dichlorophenyl)-urea and N-(3,4-dichlorophenyl)-N-methylurea through sequential N-dealkylations. In a soil bioaugmentation experiment, the inoculation of strain DP8-1 into diuron-treated soil effectively enhanced the disappearance rate of diuron.

Biodegradation of diuron by an endophytic fungus Neurospora intermedia DP8-1 isolated from sugarcane and its potential for remediating diuron-contaminated soils

Science.gov (United States)

Feng, Guojun; Du, Liangwei; Zeng, Dongqiang

2017-01-01

A diuron-degrading endophyte DP8-1 was isolated from sugarcane root grown in diuron-treated soil in the present study. The endophyte was identified as Neurospora intermedia based on the morphological characteristics and sequence analysis. The fermentation parameters including temperature, pH, inoculation size, carbon source, and initial diuron concentration were also investigated for the optimization of degradation efficiency. The results indicated that strain DP8-1 was capable of degrading up to 99% diuron within 3 days under the optimal degrading condition. The study of degradation spectrum indicated that strain DP8-1 could also degrade and utilize fenuron, monuron, metobromuron, isoproturon, chlorbromuron, linuron, and chlortoluron as substrate for strain growth. On basis of liquid chromatography-mass spectrometry analysis for the products of the degradation of diuron, strain DP8-1 metabolized diuron to produce N-(3,4-dichlorophenyl)-urea and N-(3,4-dichlorophenyl)-N-methylurea through sequential N-dealkylations. In a soil bioaugmentation experiment, the inoculation of strain DP8-1 into diuron-treated soil effectively enhanced the disappearance rate of diuron. PMID:28809955

A Novel Hyaluronidase from Brown Spider (Loxosceles intermedia) Venom (Dietrich's Hyaluronidase): From Cloning to Functional Characterization

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Ferrer, Valéria Pereira; de Mari, Thiago Lopes; Gremski, Luiza Helena; Trevisan Silva, Dilza; da Silveira, Rafael Bertoni; Gremski, Waldemiro; Chaim, Olga Meiri; Senff-Ribeiro, Andrea; Nader, Helena Bonciani; Veiga, Silvio Sanches

2013-01-01

Loxoscelism is the designation given to clinical symptoms evoked by Loxosceles spider's bites. Clinical manifestations include skin necrosis with gravitational spreading and systemic disturbs. The venom contains several enzymatic toxins. Herein, we describe the cloning, expression, refolding and biological evaluation of a novel brown spider protein characterized as a hyaluronidase. Employing a venom gland cDNA library, we cloned a hyaluronidase (1200 bp cDNA) that encodes for a signal peptide and a mature protein. Amino acid alignment revealed a structural relationship with members of hyaluronidase family, such as scorpion and snake species. Recombinant hyaluronidase was expressed as N-terminal His-tag fusion protein (∼45 kDa) in inclusion bodies and activity was achieved using refolding. Immunoblot analysis showed that antibodies that recognize the recombinant protein cross-reacted with hyaluronidase from whole venom as well as an anti-venom serum reacted with recombinant protein. Recombinant hyaluronidase was able to degrade purified hyaluronic acid (HA) and chondroitin sulfate (CS), while dermatan sulfate (DS) and heparan sulfate (HS) were not affected. Zymograph experiments resulted in ∼45 kDa lytic zones in hyaluronic acid (HA) and chondroitin sulfate (CS) substrates. Through in vivo experiments of dermonecrosis using rabbit skin, the recombinant hyaluronidase was shown to increase the dermonecrotic effect produced by recombinant dermonecrotic toxin from L. intermedia venom (LiRecDT1). These data support the hypothesis that hyaluronidase is a “spreading factor”. Recombinant hyaluronidase provides a useful tool for biotechnological ends. We propose the name Dietrich's Hyaluronidase for this enzyme, in honor of Professor Carl Peter von Dietrich, who dedicated his life to studying proteoglycans and glycosaminoglycans. PMID:23658852

Shark Spotters: Successfully reducing spatial overlap between white sharks (Carcharodon carcharias) and recreational water users in False Bay, South Africa.

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Engelbrecht, Tamlyn; Kock, Alison; Waries, Sarah; O'Riain, M Justin

2017-01-01

White sharks (Carcharodon carcharias) are apex predators that play an important role in the structure and stability of marine ecosystems. Despite their ecological importance and protected status, white sharks are still subject to lethal control to reduce the risk of shark bites for recreational water users. The Shark Spotters program, pioneered in Cape Town, South Africa, provides a non-lethal alternative for reducing the risk of human-shark conflict. In this study we assessed the efficacy of the Shark Spotters program in reducing overlap between water users and white sharks at two popular beaches in False Bay, South Africa. We investigated seasonal and diel patterns in water use and shark presence at each beach, and thereafter quantified the impact of different shark warnings from shark spotters on water user abundance. We also assessed the impact of a fatal shark incident on patterns of water use. Our results revealed striking diel and seasonal overlap between white sharks and water users at both beaches. Despite this, there was a low rate of shark-human incidents (0.5/annum) which we attribute partly to the success of the Shark Spotters program. Shark spotters use visual (coloured flags) and auditory (siren) cues to inform water users of risk associated with white shark presence in the surf zone. Our results showed that the highest risk category (denoted by a white flag and accompanying siren) caused a significant reduction in water user abundance; however the secondary risk category (denoted by a red flag with no siren) had no significant effect on water users. A fatal shark incident was shown to negatively impact the number of water users present for at least three months following the incident. Our results indicate that the Shark Spotters program effectively reduces spatial overlap between white sharks and water users when the risk of conflict is highest.

Circumpolar diversity and geographic differentiation of mtDNA in the critically endangered Antarctic blue whale (Balaenoptera musculus intermedia.

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Angela L Sremba

Full Text Available The Antarctic blue whale (Balaenoptera musculus intermedia was hunted to near extinction between 1904 and 1972, declining from an estimated initial abundance of more than 250,000 to fewer than 400. Here, we describe mtDNA control region diversity and geographic differentiation in the surviving population of the Antarctic blue whale, using 218 biopsy samples collected under the auspices of the International Whaling Commission (IWC during research cruises from 1990-2009. Microsatellite genotypes and mtDNA sequences identified 166 individuals among the 218 samples and documented movement of a small number of individuals, including a female that traveled at least 6,650 km or 131° longitude over four years. mtDNA sequences from the 166 individuals were aligned with published sequences from 17 additional individuals, resolving 52 unique haplotypes from a consensus length of 410 bp. From this minimum census, a rarefaction analysis predicted that only 72 haplotypes (95% CL, 64, 86 have survived in the contemporary population of Antarctic blue whales. However, haplotype diversity was relatively high (0.968±0.004, perhaps as a result of the longevity of blue whales and the relatively recent timing of the bottleneck. Despite the potential for circumpolar dispersal, we found significant differentiation in mtDNA diversity (F(ST = 0.032, p<0.005 and microsatellite alleles (F(ST = 0.005, p<0.05 among the six Antarctic Areas historically used by the IWC for management of blue whales.

Identification, validation and cross-species transferability of novel Lavandula EST-SSRs.

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Adal, Ayelign M; Demissie, Zerihun A; Mahmoud, Soheil S

2015-04-01

We identified and characterized EST-SSRs with strong discrimination power against Lavandula angustifolia and Lavandula x intermedia . The markers also showed considerable cross-species transferability rate into six related Lavandula species. Lavenders (Lavandula) are important economical crops grown around the globe for essential oil production. In an attempt to develop genetic markers for these plants, we analyzed over 13,000 unigenes developed from L. angustifolia and L. x intermedia EST databases, and identified 3,459 simple sequence repeats (SSR), which were dominated by trinucleotides (41.2 %) and dinucleotides (31.45 %). Approximately, 19 % of the unigenes contained at least one SSR marker, over 60 % of which were localized in the UTRs. Only 252 EST-SSRs were 18 bp or longer from which 31 loci were validated, and 24 amplified discrete fragments with 85 % polymorphism in L. x intermedia and L. angustifolia. The average number of alleles in L. x intermedia and L. angustifolia were 3.42 and 3.71 per marker with average PIC values of 0.47 and 0.52, respectively. These values suggest a moderate to strong level of informativeness for the markers, with some loci producing unique fingerprints. The cross-species transferability rate of the markers ranges 50-100 % across eight species. The utility of these markers was assessed in eight Lavandula species and 15 L. angustifolia and L. x intermedia cultivars, and the dendrogram deduced from their similarity indexes successfully delineated the species into their respective sections and the cultivars into their respective species. These markers have potential for application in fingerprinting, diversity studies and marker-assisted breeding of Lavandula.

Development of SNS Stream Analysis Based on Forest Disaster Warning Information Service System

Science.gov (United States)

Oh, J.; KIM, D.; Kang, M.; Woo, C.; Kim, D.; Seo, J.; Lee, C.; Yoon, H.; Heon, S.

2017-12-01

Forest disasters, such as landslides and wildfires, cause huge economic losses and casualties, and the cost of recovery is increasing every year. While forest disaster mitigation technologies have been focused on the development of prevention and response technologies, they are now required to evolve into evacuation and border evacuation, and to develop technologies fused with ICT. In this study, we analyze the SNS (Social Network Service) stream and implement a system to detect the message that the forest disaster occurred or the forest disaster, and search the keyword related to the forest disaster in advance in real time. It is possible to detect more accurate forest disaster messages by repeatedly learning the retrieved results using machine learning techniques. To do this, we designed and implemented a system based on Hadoop and Spark, a distributed parallel processing platform, to handle Twitter stream messages that open SNS. In order to develop the technology to notify the information of forest disaster risk, a linkage of technology such as CBS (Cell Broadcasting System) based on mobile communication, internet-based civil defense siren, SNS and the legal and institutional issues for applying these technologies are examined. And the protocol of the forest disaster warning information service system that can deliver the SNS analysis result was developed. As a result, it was possible to grasp real-time forest disaster situation by real-time big data analysis of SNS that occurred during forest disasters. In addition, we confirmed that it is possible to rapidly propagate alarm or warning according to the disaster situation by using the function of the forest disaster warning information notification service. However, the limitation of system application due to the restriction of opening and sharing of SNS data currently in service and the disclosure of personal information remains a problem to be solved in the future. Keyword : SNS stream, Big data, Machine

DESEMPEÑO DE UNA PELÍCULA DE MAÍZ AZUL EN EL ENVASADO DE UN QUESO DE HUMEDAD INTERMEDIA

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CECILIA ROJAS DE G.

Full Text Available La PCT/MX2009/000092, describe un método para obtener películas a partir de harina de cereales mediante método de "casting" y empleocomo almohadillas antimicrobianas yenvases termosellables para alimentos secos. Debido a su sensibilidad al agua y baja resistencia mecánica para contener alimentos de humedad intermedia; se reformuló la matriz biopolimérica para mejorar ambos parámetros.La película derivada de la reformulación fue caracterizada mecánicamente (ASTM D1708, se midió el "swelling" y se observó mediante microscopía electrónica de barrido.El desempeño de la película fue evaluado envasando unalimento perecedero: un quesosemimadurado,sometiéndolo a almacenamiento refrigerado durante 2 meses. Control: se emplearon queso del mismo lote envasado al vacío en película alta barrera. Durante los estudios de estabilidad en anaquel se evaluaron parámetros críticos microbiológicos como fisicoquímicos para el alimento envasado.Se observó que si bien las películas activas controlaron la carga microbiana, la integridad del envase se vio alterada debido a absorción de agua por contacto con el alimento, observándose ruptura de la estructura e integridad del biopolímeroy en consecuencia, exposición del producto y término de su vida útil.

The responses of an anaerobic microorganism, Yersinia intermedia MASE-LG-1 to individual and combined simulated Martian stresses.

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Kristina Beblo-Vranesevic

Full Text Available The limits of life of aerobic microorganisms are well understood, but the responses of anaerobic microorganisms to individual and combined extreme stressors are less well known. Motivated by an interest in understanding the survivability of anaerobic microorganisms under Martian conditions, we investigated the responses of a new isolate, Yersinia intermedia MASE-LG-1 to individual and combined stresses associated with the Martian surface. This organism belongs to an adaptable and persistent genus of anaerobic microorganisms found in many environments worldwide. The effects of desiccation, low pressure, ionizing radiation, varying temperature, osmotic pressure, and oxidizing chemical compounds were investigated. The strain showed a high tolerance to desiccation, with a decline of survivability by four orders of magnitude during a storage time of 85 days. Exposure to X-rays resulted in dose-dependent inactivation for exposure up to 600 Gy while applied doses above 750 Gy led to complete inactivation. The effects of the combination of desiccation and irradiation were additive and the survivability was influenced by the order in which they were imposed. Ionizing irradiation and subsequent desiccation was more deleterious than vice versa. By contrast, the presence of perchlorates was not found to significantly affect the survival of the Yersinia strain after ionizing radiation. These data show that the organism has the capacity to survive and grow in physical and chemical stresses, imposed individually or in combination that are associated with Martian environment. Eventually it lost its viability showing that many of the most adaptable anaerobic organisms on Earth would be killed on Mars today.

The responses of an anaerobic microorganism, Yersinia intermedia MASE-LG-1 to individual and combined simulated Martian stresses

Science.gov (United States)

Bohmeier, Maria; Perras, Alexandra K.; Schwendner, Petra; Rabbow, Elke; Moissl-Eichinger, Christine; Cockell, Charles S.; Pukall, Rüdiger; Vannier, Pauline; Marteinsson, Viggo T.; Monaghan, Euan P.; Ehrenfreund, Pascale; Garcia-Descalzo, Laura; Gómez, Felipe; Malki, Moustafa; Amils, Ricardo; Gaboyer, Frédéric; Westall, Frances; Cabezas, Patricia; Walter, Nicolas; Rettberg, Petra

2017-01-01

The limits of life of aerobic microorganisms are well understood, but the responses of anaerobic microorganisms to individual and combined extreme stressors are less well known. Motivated by an interest in understanding the survivability of anaerobic microorganisms under Martian conditions, we investigated the responses of a new isolate, Yersinia intermedia MASE-LG-1 to individual and combined stresses associated with the Martian surface. This organism belongs to an adaptable and persistent genus of anaerobic microorganisms found in many environments worldwide. The effects of desiccation, low pressure, ionizing radiation, varying temperature, osmotic pressure, and oxidizing chemical compounds were investigated. The strain showed a high tolerance to desiccation, with a decline of survivability by four orders of magnitude during a storage time of 85 days. Exposure to X-rays resulted in dose-dependent inactivation for exposure up to 600 Gy while applied doses above 750 Gy led to complete inactivation. The effects of the combination of desiccation and irradiation were additive and the survivability was influenced by the order in which they were imposed. Ionizing irradiation and subsequent desiccation was more deleterious than vice versa. By contrast, the presence of perchlorates was not found to significantly affect the survival of the Yersinia strain after ionizing radiation. These data show that the organism has the capacity to survive and grow in physical and chemical stresses, imposed individually or in combination that are associated with Martian environment. Eventually it lost its viability showing that many of the most adaptable anaerobic organisms on Earth would be killed on Mars today. PMID:29069099

76 FR 35235 - Endangered and Threatened Species Permit Applications

Science.gov (United States)

2011-06-16

... chub (Gila intermedia), Gila topminnow (Poeciliopsis occidentalis occidentalis), humpback chub (Gila... intermedia) within Arizona. Permit TE-43777A Applicant: Sea Life US, LLC, Grapevine, Texas. Applicant...), and Gila chub (Gila intermedia) within Arizona. Permit TE-118414 Applicant: Cherokee Nation, Tahlequah...

Raíz secundaria embrional asociada a la germinación de Garcinia intermedia (Clusiaceae y su posible papel en la supervivencia de las plántulas

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José Fco Di Stefano

2006-09-01

Full Text Available En pruebas preliminares de germinación con semillas de Garcinia intermedia (Clusiaceae se observó que ésta producía dos tipos de raíces: la primaria y una secundaria embrional que atraviesa longitudinalmente la semilla. Para determinar su posible papel en la supervivencia y crecimiento de esta especie, se analizaron 90 plántulas con al menos seis meses de germinadas en bolsas plásticas con suelo orgánico ubicadas en un invernadero, a las que se les aplicó uno de los siguientes tratamientos: corte de la raíz primaria o la secundaria embrional, además de un grupo control con ambas raíces (30 repeticiones de cada uno. Luego de tres meses se empezaron a extraer 10 plántulas/mes/tratamiento a los que se les midió la altura total, diámetro basal, longitud de la raíz primaria y secundaria, y la biomasa del vástago, raíz y semilla sin la cubierta seminal. Las plántulas que obtuvieron el mayor crecimiento fueron las del control, seguidas por aquéllas sin la raíz secundaria. Sin embargo, más del 90% de las plántulas a las que se les cortó la raíz primaria, sobrevivieron después de cinco meses de haberse aplicado el tratamiento, en parte debido a una muy alta capacidad de recuperación de su sistema radical, ya sea produciendo rebrotes de la principal (20%, aumentando el crecimiento de la raíz secundaria (13.3%, o ambos (26.7%, y a las reservas de la semilla. La longitud de los rebrotes de la raíz principal fue estadísticamente significativa en las primeras dos extracciones mensuales de plantas, comparado con las del control (F6= 18.6, F7= 16.0, pSecondary embryonary root associated with seed germination in Garcinia intermedia (Clusiaceae and its possible role in seedling survival. Germination tests on Garcinia intermedia (Clusiaceae seeds showed the growth of two types of roots: additionally to the primary root, a secondary root crosses the seed lengthwise. To determine its possible role on the survival and growth of this

Framework for Multi-Pathway Cumulative Exposure for Comparative Assessments

DEFF Research Database (Denmark)

McKone, Tom; Fantke, Peter

2016-01-01

in comparative risk assessment, life-cycle assessment (LCA), and chemical alternatives assessment (CAA), multimedia fate and exposure models synthesize information about partitioning, reaction, and intermedia-transport properties of chemicals in a representative (local to regional) or generic (continental...

An intelligent IoT emergency vehicle warning system using RFID and Wi-Fi technologies for emergency medical services.

Science.gov (United States)

Lai, Yeong-Lin; Chou, Yung-Hua; Chang, Li-Chih

2018-01-01

Collisions between emergency vehicles for emergency medical services (EMS) and public road users have been a serious problem, impacting on the safety of road users, emergency medical technicians (EMTs), and the patients on board. The aim of this study is to develop a novel intelligent emergency vehicle warning system for EMS applications. The intelligent emergency vehicle warning system is developed by Internet of Things (IoT), radio-frequency identification (RFID), and Wi-Fi technologies. The system consists of three major parts: a system trigger tag, an RFID system in an emergency vehicle, and an RFID system at an intersection. The RFID system either in an emergency vehicle or at an intersection contains a controller, an ultrahigh-frequency (UHF) RFID reader module, a Wi-Fi module, and a 2.4-GHz antenna. In addition, a UHF ID antenna is especially designed for the RFID system in an emergency vehicle. The IoT system provides real-time visual warning at an intersection and siren warning from an emergency vehicle in order to effectively inform road users about an emergency vehicle approaching. The developed intelligent IoT emergency vehicle warning system demonstrates the capabilities of real-time visual and siren warnings for EMS safety.

A Development of Common Cause Failure Propagation Paths Identification Method Using Coloured Petri Nets

International Nuclear Information System (INIS)

Yim, Ho Bin; Park, Jae Min; Lee, Chang Gyun; Huh, Jae Young; Lee, Gyu Cheon

2017-01-01

The concept of Common-Cause Failure (CCF) first appeared in the aerospace industry several decades ago, and nuclear power industry actively adopted the concept to the nuclear power plant (NPP) system analysis after the TMI accident. Since digital Instrumentation and Control (I and C) systems were applied to the NPP design, the CCF issues once again drew attention from the nuclear power industry in 90's. Identification of CCF has not been considered as a challenging issue because of its simplicity. However, as the systems become more complex and interconnected, demands are increasing to analyze CCF in more detail, for example, CCF with multiple initiating events or supporting situation awareness of the operation crew. The newly suggested CCF propagation paths identification method, CCF-SIREn, is expected to resolve path identification issue more practically and efficiently. CCF-SIREn uses general diagrams so that the compatibility and usability can be hugely increased. It also offers up-to-date CCF information with a least analysis effort whenever the ordinary NPP design change processes are made. A back-propagation technique is still under development to find out root-causes from the suspiciously responding signals, alarms and components. The probabilistic approach is also under consideration to prioritize defined CCF.

22nd Italian Workshop on Neural Nets

CERN Document Server

Bassis, Simone; Esposito, Anna; Morabito, Francesco

2013-01-01

This volume collects a selection of contributions which has been presented at the 22nd Italian Workshop on Neural Networks, the yearly meeting of the Italian Society for Neural Networks (SIREN). The conference was held in Italy, Vietri sul Mare (Salerno), during May 17-19, 2012. The annual meeting of SIREN is sponsored by International Neural Network Society (INNS), European Neural Network Society (ENNS) and IEEE Computational Intelligence Society (CIS). The book – as well as the workshop-  is organized in three main components, two special sessions and a group of regular sessions featuring different aspects and point of views of artificial neural networks and natural intelligence, also including applications of present compelling interest.

Primer aislamiento en Argentina de Staphylococcus aureus resistente a la meticilina adquirido en la comunidad con sensibilidad intermedia a la vancomicina y no sensibilidad a la daptomicina

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Laura Errecalde

Full Text Available Describimos el primer caso en Argentina de Staphylococcus aureus resistente a la meticilina adquirido en la comunidad con sensibilidad intermedia a la vancomicina y no sensibilidad a la daptomicina. Caso clínico: Paciente masculino, con antecedente de insuficiencia renal crónica en hemodiálisis y osteosíntesis de cadera debido a una fractura. Se internó por síndrome febril persistente luego del desplazamiento de la prótesis por un traumatismo. Se aisló de hemocultivos S. aureus resistente a la meticilina adquirido en la comunidad. Durante el tratamiento con vancomicina y daptomicina, se observó un aumento gradual de la CIM de vancomicina de 1 µg/ml (VSSA a 2 µg/ml (h-VISA y a 4 µg/ml (VISA, y la emergencia de no sensibilidad a daptomicina (CIM = 4 µg/ml. Al suspender la vancomicina y la daptomicina, la cepa revirtió al fenotipo de sensibilidad a ambas drogas. Es mandatorio evaluar por CIM la sensibilidad a vancomicina y a daptomicina intratratamiento cuando estas drogas se usan como terapia.

Sistema constructivo de tecnología intermedia como solución al problema de vivienda en las áreas marginales y socioeconómicamente deprimidas de una ciudad

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Alva Mejía, Alberto

1984-07-01

Full Text Available The constructive system of intermediate technology offers to the population the possibility to construct his dwelling by the way of autoconstruction in stages. Its aim is to project and construct at least 10 % of the demands for housing in Lima, within a three years limit, and to support the creation of Communal Crafts Workshops. The system is to join several prefabricated elements as girders, sheets and concrete arches with vegetal fibres, wooden roof frames, etc.El sistema constructivo de tecnología intermedia ofrece a la población la posibilidad de construir su vivienda bajo la modalidad de autoconstrucción por etapas. Su meta es proyectar y construir, al menos, el 10 % de las viviendas demandadas en la actualidad en Lima con un plazo de 3 años, y apoyar la formación de talleres artesanales comunales. El sistema consiste en la unión de varios elementos prefabricados como son viguetas, láminas y arcos de hormigón con fibras vegetales, tijerales de madera, etcétera.

Therapeutic superiority and safety of combined hydroxyurea with recombinant human erythropoietin over hydroxyurea in young β-thalassemia intermedia patients.

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Elalfy, Mohsen S; Adly, Amira A M; Ismail, Eman A; Elhenawy, Yasmine I; Elghamry, Islam R

2013-12-01

To assess the efficacy and safety of combined hydroxyurea (HU) and recombinant human erythropoietin (rHuEPO) in β-thalassemia intermedia (TI) patients compared with single HU therapy. An interventional prospective randomized study registered in the ClinicalTrials.gov (NCT01624038) was performed on 80 TI patients (≤ 18 yr) divided into group A (40 patients received combined HU and rHuEPO) and group B (40 patients received single HU therapy). Baseline serum EPO levels were measured, and both groups were followed up for a mean period of 1 yr with regular assessment of transfusion requirements, blood pressure, ferritin, liver and renal functions, hemoglobin, and HbF. Quality of life (QoL) was assessed at the start and end of the study. Transfusion frequency and index were significantly decreased, while QoL was increased in group A compared with group B where 85% of patients showed improvement on combined therapy compared with 50% of patients on HU. Hemoglobin and HbF were significantly increased in both TI groups; however, this was more evident in group A than in group B. Also, 37.5% of patients in group A became transfusion-independent compared with 15% in group B. EPO levels were negatively related to increments of hemoglobin and HbF. Splenectomized patients and those with initial HbF% >40% had the best response to combined therapy. No serious adverse events necessitating discontinuation of therapy in both groups. HU was effective in management of TI; however, combination with rHuEPO gave a superior therapeutic effect resulting in the best clinical and hematological responses without adverse events. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Evolución de estrellas enanas blancas de Helio de masa baja e intermedia

Science.gov (United States)

Althaus, L. G.; Benvenuto, O. G.

Numerosas observaciones realizadas particularmente en los últimos dos años parecen confirmar que las enanas blancas (EB) de helio de masa baja e intermedia son el resultado de la evolución de sistemas binarios cercanos. Con el objeto de realizar una adecuada interpretación de estos objetos son necesarios modelos de EBs de helio lo más detallado posibles. En este estudio presentamos cálculos detallados de la evolución de EBs de helio con masas entre M=0.1Msolar y M=0.5Msolar a intervalos de 0.05Msolar . Para ello, hemos tenido en cuenta los efectos de temperatura finita mediante un código de evolución estelar lo más actualizado posible. En particular, el transporte de energía es descripto en el marco del nuevo modelo para la convección turbulenta desarrollado por Canuto - Mazzitelli. Además hemos considerado la nueva ecuación de estado para plasmas de helio de Saumon et al. y nuevas opacidades radiativas OPAL. Las pérdidas por neutrinos fueron asimismo tenidas en cuenta. Excepto para las EBs más masivas, nuestros modelos iniciales están ubicados en las cercanías de la correspondiente línea de Hayashi para configuraciones de helio. Nuestros resultados muestran que existe una región prohibida en el diagrama observacional HR donde ninguna EB de helio puede encontrarse. Dicha región es para log{(L/Lsolar )}>= -0.25 and log{Teff} >= 4.45. Hemos encontrado también que los tracks evolutivos en el diagrama HR en el dominio de alta luminosidad (pre - EB) son fuertemente afectados por la eficiencia convectiva y que las pérdidas por neutrinos son importantes en los modelos más masivos. Finalmente hemos analizado la estructura de la zona convectiva externa encontrando que la teoría de Canuto - Mazzitelli conduce a un perfil convectivo muy diferente del dado por cualquier versión de la popular teoría de la mixing length. Si bién este comportamiento es decisivo en el contexto de las inestabilides pulsacionales, los radios y gravedades superficiales de

Does your profile say it all? Using demographics to predict expressive head movement during gameplay

DEFF Research Database (Denmark)

Asteriadis, Stylianos; Karpouzis, Kostas; Shaker, Noor

2012-01-01

interest (when the player loses during game play). Experi- ments were conducted on the Siren database, which consists of 58 par- ticipants, playing a modi¯ed version of the Super Mario. Here, as player demographics are considered the gender and age, while the statistical importance of certain facial cues......In this work, we explore the relation between expressive head movement and user pro¯le information in game play settings. Facial ges- ture analysis cues are statistically correlated with players' demographic characteristics in two di®erent settings, during game-play and at events of special...

Comments on the variation of spike morphology in selected species of Elytrigia and Elymus (Triticeae

Directory of Open Access Journals (Sweden)

Romuald Kosina

2014-01-01

Full Text Available The structure of spikes of Elytrigia repens, E. intermedia and Elymus caninus was investigated. The number of spikelets per spike reveals the weakest correlations with other characters of the spike. The same concerns some character ratios. The correlations provide information about the segmented structure (metamers of the spike. There is a great difference between matrices of correlation coefficients for E. repens and E. intermedia related to the development and structure of spike. Characters important for the description of the spike were chosen - in five-character set these are among others: length of glume awn in median spikelet, length of lemma awn in the first floret of the median spikelet, number of spikelets per spike. Length of lemma awn and mean length of the rachis segment were recognized as the best discriminants for species. Ordination of forms along axes of canonical variates does not indicate the subunits within E. repens. Intermediate forms between E. repens and Elymus caninus have not been found. Between E. repens and E. intermedia there exists some proximity. Heteromorphic individuals were described by means of cluster analysis. They prove the mobility of the genome in ramets of a single genet.

Plaadid / Jüri F. Lotman

Index Scriptorium Estoniae

Lotman, Jüri F.

2005-01-01

Uutest heliplaatidest Asian Dub Foundation "Tank", Wilco "A Ghost Is Born", New Order "Waiting For The Sirens Call", Athlete "Tourist", Cristina Branco "Ulisses", Gruff Rhys "Yr Atal Genhedlaeth", Billy Idol "Devil's Playground"

Kuu plaat : Beck "Guero". Plaadid kauplusest Lasering

Index Scriptorium Estoniae

2005-01-01

Heliplaatidest: Beck "Guero", Common "Be", Stupid F ja Hash "Koodeks: R.L.V.", Kulo "Onarhia", Natalie Imbruglia "Counting Down the Days", New Order "Waiting for the Sirens' Call", Human Ground "Human Ground"

Show and Tell: Video Modeling and Instruction Without Feedback Improves Performance but Is Not Sufficient for Retention of a Complex Voice Motor Skill.

Science.gov (United States)

Look, Clarisse; McCabe, Patricia; Heard, Robert; Madill, Catherine J

2018-02-02

Modeling and instruction are frequent components of both traditional and technology-assisted voice therapy. This study investigated the value of video modeling and instruction in the early acquisition and short-term retention of a complex voice task without external feedback. Thirty participants were randomized to two conditions and trained to produce a vocal siren over 40 trials. One group received a model and verbal instructions, the other group received a model only. Sirens were analyzed for phonation time, vocal intensity, cepstral peak prominence, peak-to-peak time, and root-mean-square error at five time points. The model and instruction group showed significant improvement on more outcome measures than the model-only group. There was an interaction effect for vocal intensity, which showed that instructions facilitated greater improvement when they were first introduced. However, neither group reproduced the model's siren performance across all parameters or retained the skill 1 day later. Providing verbal instruction with a model appears more beneficial than providing a model only in the prepractice phase of acquiring a complex voice skill. Improved performance was observed; however, the higher level of performance was not retained after 40 trials in both conditions. Other prepractice variables may need to be considered. Findings have implications for traditional and technology-assisted voice therapy. Copyright © 2017 The Voice Foundation. Published by Elsevier Inc. All rights reserved.

Assessment of tissue-specific cortisol activity with regard to degeneration of the suspensory ligaments in horses with pituitary pars intermedia dysfunction.

Science.gov (United States)

Hofberger, Sina C; Gauff, Felicia; Thaller, Denise; Morgan, Ruth; Keen, John A; Licka, Theresia F

2018-02-01

OBJECTIVE To identify signs of tissue-specific cortisol activity in samples of suspensory ligament (SL) and neck skin tissue from horses with and without pituitary pars intermedia dysfunction (PPID). SAMPLE Suspensory ligament and neck skin tissue samples obtained from 26 euthanized horses with and without PPID. PROCEDURES Tissue samples were collected from 12 horses with and 14 horses without PPID (controls). Two control horses had received treatment with dexamethasone; data from those horses were not used in statistical analyses. The other 12 control horses were classified as old horses (≥ 14 years old) and young horses (≤ 9 years old). Standard histologic staining, staining for proteoglycan accumulation, and immunostaining of SL and neck skin tissue sections for glucocorticoid receptors, insulin, 11β hydroxysteroid dehydrogenase type 1, and 11β hydroxysteroid dehydrogenase type 2 were performed. Findings for horses with PPID were compared with findings for young and old horses without PPID. RESULTS Compared with findings for old and young control horses, there were significantly more cells stained for glucocorticoid receptors in SL samples and for 11 β hydroxysteroid dehydrogenase type 1 in SL and skin tissue samples from horses with PPID. Insulin could not be detected in any of the SL or skin tissue samples. Horses with PPID had evidence of SL degeneration with significantly increased proteoglycan accumulation. Neck skin tissue was found to be significantly thinner in PPID-affected horses than in young control horses. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that tissue-specific dysregulation of cortisol metabolism may contribute to the SL degeneration associated with PPID in horses.

Seasonal and Diel Vocalization Patterns of Antarctic Blue Whale (Balaenoptera musculus intermedia in the Southern Indian Ocean: A Multi-Year and Multi-Site Study.

Directory of Open Access Journals (Sweden)

Emmanuelle C Leroy

Full Text Available Passive acoustic monitoring is an efficient way to provide insights on the ecology of large whales. This approach allows for long-term and species-specific monitoring over large areas. In this study, we examined six years (2010 to 2015 of continuous acoustic recordings at up to seven different locations in the Central and Southern Indian Basin to assess the peak periods of presence, seasonality and migration movements of Antarctic blue whales (Balaenoptera musculus intermedia. An automated method is used to detect the Antarctic blue whale stereotyped call, known as Z-call. Detection results are analyzed in terms of distribution, seasonal presence and diel pattern of emission at each site. Z-calls are detected year-round at each site, except for one located in the equatorial Indian Ocean, and display highly seasonal distribution. This seasonality is stable across years for every site, but varies between sites. Z-calls are mainly detected during autumn and spring at the subantarctic locations, suggesting that these sites are on the Antarctic blue whale migration routes, and mostly during winter at the subtropical sites. In addition to these seasonal trends, there is a significant diel pattern in Z-call emission, with more Z-calls in daytime than in nighttime. This diel pattern may be related to the blue whale feeding ecology.

Pop / Mart Juur

Index Scriptorium Estoniae

Juur, Mart, 1964-

2005-01-01

Heliplaatidest: Athlete "Tourist", The Phazz "Natural Fake", David Sylvian "The Good Son Vs The Only Daughter: The Blemish Remixes", Konono No. 1 "Congotronics", Halo "Värvid", Rufus Wainwright "Want Two", New Order "Waiting For The Sirens' Call"

Hearing Loss

Science.gov (United States)

... Risk range 80 to 90 Heavy city traffic, power lawn mower 90 Motorcycle 100 Snowmobile, hand drill 110 Chain saw, rock concert Injury range 120 Ambulance siren 140 (pain threshold) Jet engine at takeoff 165 12-guage ...

Summary of the 2010 assessment on medium- to low-temperature geothermal resources in Mexico; Resumen de la evaluacion 2010 de los recursos geotermicos mexicanos de temperatura intermedia a baja

Energy Technology Data Exchange (ETDEWEB)

Iglesias, Eduardo R.; Torres, Rodolfo J.; Martinez Estrella, J. Ignacio; Reyes Picasso, Neftali [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)]. E-mail: iglesias@iie.org.mx

2011-07-15

In 2003 we published our first assessment of the medium- to low-temperature (T {<=} 200 degrees Celsius) Mexican geothermal resources. The assessment was based on a database of 1,358 geothermal manifestations (surface manifestations, e.g. springs, fumaroles, water wells, etc.) identified at that time. Due to a lack of information on one or more relevant parameters, such as geographical coordinates, reservoir or surface temperatures, types of fluid, etc., that assessment included only about 30% of the geothermal manifestations in the database. Since then our group has increased significantly the amount of information in the database, using field work and data compilation from different sources. We have developed a database linked with a Geographical Information System (GIS). This work presents an updated assessment of the medium- to low-temperature Mexican geothermal resources based on our current database, which includes 2,361 geothermal manifestations. As before, we have relied on the volume method and Montecarlo simulations to estimate geothermal resources and their uncertainties for each identified geothermal system. These geothermal systems very often include more than one geothermal manifestation, generally increasing the reliability of the individual estimations. In all, we estimated the geothermal resources of 918 individual geothermal systems which included 1,797 geothermal manifestations (as before, a significant fraction of the identified manifestations lack relevant information) located in 26 of the 32 Mexican States. In most cases these resources would be classified as inferred resources, according to the Australian Geothermal Code. We then added the inferred thermal-energy statistical distributions of the 918 geothermal systems by Montecarlo simulation, obtaining the total estimated geothermal resources of the 26 Mexican States and its uncertainty. With the resulting statistical distribution, we estimated the total-thermal energy stored in the 918

Acoustic Levitation System

Science.gov (United States)

Gammell, P. M.; Wang, T. G.; Croonquist, A.; Lee, M. C.

1985-01-01

Dense materials, such as steel balls, continuously levitated with energy provided by efficient high-powered siren in combination with shaped reflector. Reflector system, consisting of curved top reflector and flat lower reflector, eliminates instability in spatial positioning of sample.

For easy sleep along the shore: Making hazard warnings more ...

International Development Research Centre (IDRC) Digital Library (Canada)

2011-01-27

Jan 27, 2011 ... For easy sleep along the shore: Making hazard warnings more effective ... to alert the public; local leaders trained to make the right decisions; .... and the sirens sound, its people will have the motivation and the capacity to act?

Diagnostic accuracy and therapeutic impact of endoscopic ultrasonography in patients with intermediate suspicion of choledocholithiasis and absence of findings in magnetic resonance cholangiography Precisión diagnóstica e impacto terapéutico de la ultrasonografía endoscópica en los pacientes con sospecha intermedia de coledocolitiasis y colangiorresonancia normal

Directory of Open Access Journals (Sweden)

Enrique Vázquez-Sequeiros

2011-09-01

Full Text Available Background: endoscopic ultrasonography (EUS and magnetic resonance cholangiography (MRC are the elective tests in the diagnosis of choledocholithiasis. MRC is best accepted by patients, but its sensitivity might decrease in the evaluation of microlithiasis. Aim: to evaluate the diagnostic accuracy and therapeutic impact of EUS in a prospective cohort of patients with intermediate suspicion of choledocolithiasis and no findings in MRC (normal MRC. Material and methods: during a period of 24 months, all the patients with clinical intermediate suspicion of choledocholithiasis and normal MRC were included. Sensitivity, specificity, diagnostic accuracy, positive predictive value (PPV and negative predictive value (NPV of MRC and EUS were compared, and so their impact in the management of these patients. Results: seventy six patients were evaluated (lithiasis in 30% of them. Sensitivity and diagnostic accuracy of EUS (100%, 92% were significantly higher than MRC values (0%, 70% (p Antecedentes: la ultrasonografía endoscópica (USE y la colangiografía por resonancia magnética nuclear (CRM son las técnicas de elección para el diagnóstico de litiasis en la vía biliar. Aunque la CRM es mejor aceptada por los pacientes, la sensibilidad de esta para detectar litiasis de pequeño tamaño podría ser insuficiente. Objetivo: estudiar de forma prospectiva la precisión diagnóstica y el impacto terapéutico de la USE en los pacientes con sospecha intermedia de coledocolitiasis y CRM normal. Material y métodos: se incluyeron prospectivamente durante 24 meses todos los pacientes con sospecha clínica intermedia de coledocolitiasis y CRM negativa. Se compar�� la sensibilidad, especificidad, precisión diagnóstica, valor predictivo positivo y negativo de CRM y USE, así como su impacto en el tratamiento. Resultados: se evaluaron 76 pacientes (litiasis en el 30%. La sensibilidad y precisión diagnóstica de USE (100%, 92% eran significativamente

Genetic Variants at BCL11A and HBS1L-MYB loci Influence Hb F Levels in Chinese Zhuang β-Thalassemia Intermedia Patients.

Science.gov (United States)

Lai, Yunli; Chen, Yun; Chen, Biyan; Zheng, Haiyang; Yi, Sheng; Li, Guojian; Wei, Hongwei; He, Sheng; Zheng, Chenguang

2016-11-01

Increased Hb F levels can ameliorate the symptoms of β-thalassemia (β-thal). Due to the genetic heterogenicity of β-thal, the relationship between genetic variants in modifier genes and Hb F level has been studied in different populations. The Chinese Zhuang has the second largest population in China and has 6.78% prevalence of β-thal. However, the effects of these single nucleotide polymorphism (SNP) variants on the Hb F levels of β-thal intermedia (β-TI) patients in this population have not been reported. To explore the association between modifier loci (β-globin gene cluster, HBS1L-MYB intergenic region and BCL11A) and Hb F levels in Chinese Zhuang β-TI patients, 96 unrelated β-TI patients (50 males and 46 females) with different Hb F levels were recruited and genotyped by mass spectrometry. A total of 13 SNPs were confirmed to be in a significant relationship with Hb F levels in this population. Of these, high-risk genotypes of six Hb F-associated SNPs, rs9376090, rs7776054, rs9399137, rs9389268, rs9402685 in the HBS1L-MYB intergenic region and rs189984760 in the BCL11A locus, showed association with high Hb F levels, especially for SNPs in linkage disequilibrium. One novel Hb F-associated SNP, rs189984760, was identified in our study. Our findings will be of valuable reference for correlation between modifier genes and Hb F in Chinese Zhuang populations and may lead to better understand the modifying mechanisms for β-thal.

Lignans From Forsythia x Intermedia Leaves and Flowers Attenuate the Pro-inflammatory Function of Leukocytes and Their Interaction With Endothelial Cells

Science.gov (United States)

Michalak, Barbara; Filipek, Agnieszka; Chomicki, Piotr; Pyza, Małgorzata; Woźniak, Marta; Żyżyńska-Granica, Barbara; Piwowarski, Jakub P.; Kicel, Agnieszka; Olszewska, Monika A.; Kiss, Anna K.

2018-01-01

Aim of the study: Taking into account that overactivated leukocytes are an important factor in the development of many chronic diseases, we investigated the activity of phytochemically characterized (HPLC-DAD-MSn) extracts from forsythia leaves and flowers on the pro- and anti-inflammatory functions of leukocytes (effects on IL-1β, IL-8, TNF-α, and TGFβ release) and their adherence to endothelial cells. Using bio-guided fractionation, we isolated the active compounds and determined their biological activity, and we included the positive control quercetin. Methods: The effect on IL-1β, TNF-α, IL-8, and TGF-α production by leukocytes was measured by enzyme-linked immunosorbent assay (ELISA). The surface expression of adhesion molecules was analyzed with flow cytometry, and the neutrophil attachment to the endothelial cells was assessed fluorimetrically. The effects on p38MAPK, ERK1/2 and JNK phosphorylation were determined using western blots. Results: Leaf extracts had the effect of decreasing TNF-α production in neutrophils and monocyte/macrophage cells. The bio-guided fractionation led to the isolation of the following lignan aglycones: (+)-pinoresinol, (+)-epipinoresinol, (−)-matairesinol, (+)-phillygenin, and (−)-arctigenin. Only phillygenin was able to stimulate the anti-inflammatory function of macrophages by inducing TGF-β release and IL-10 receptor surface expression. Arctigenin, phillygenin, and a metabolite produced by the gut microbiota, enterolactone, decreased TNF-α and IL-1β production and neutrophil adhesion to endothelial cells, probably by attenuating the p38 and ERK kinase pathways. Conclusion: Forsythia x intermedia is a valuable source of active lignans, which may be potential candidates for treating inflammatory diseases that are associated with the excessive production of cytokines such as TNF-α and IL-1β. PMID:29740324

Lignans From Forsythia x Intermedia Leaves and Flowers Attenuate the Pro-inflammatory Function of Leukocytes and Their Interaction With Endothelial Cells

Directory of Open Access Journals (Sweden)

Barbara Michalak

2018-04-01

Full Text Available Aim of the study: Taking into account that overactivated leukocytes are an important factor in the development of many chronic diseases, we investigated the activity of phytochemically characterized (HPLC-DAD-MSn extracts from forsythia leaves and flowers on the pro- and anti-inflammatory functions of leukocytes (effects on IL-1β, IL-8, TNF-α, and TGFβ release and their adherence to endothelial cells. Using bio-guided fractionation, we isolated the active compounds and determined their biological activity, and we included the positive control quercetin.Methods: The effect on IL-1β, TNF-α, IL-8, and TGF-α production by leukocytes was measured by enzyme-linked immunosorbent assay (ELISA. The surface expression of adhesion molecules was analyzed with flow cytometry, and the neutrophil attachment to the endothelial cells was assessed fluorimetrically. The effects on p38MAPK, ERK1/2 and JNK phosphorylation were determined using western blots.Results: Leaf extracts had the effect of decreasing TNF-α production in neutrophils and monocyte/macrophage cells. The bio-guided fractionation led to the isolation of the following lignan aglycones: (+-pinoresinol, (+-epipinoresinol, (−-matairesinol, (+-phillygenin, and (−-arctigenin. Only phillygenin was able to stimulate the anti-inflammatory function of macrophages by inducing TGF-β release and IL-10 receptor surface expression. Arctigenin, phillygenin, and a metabolite produced by the gut microbiota, enterolactone, decreased TNF-α and IL-1β production and neutrophil adhesion to endothelial cells, probably by attenuating the p38 and ERK kinase pathways.Conclusion:Forsythia x intermedia is a valuable source of active lignans, which may be potential candidates for treating inflammatory diseases that are associated with the excessive production of cytokines such as TNF-α and IL-1β.

Lignans From Forsythia x Intermedia Leaves and Flowers Attenuate the Pro-inflammatory Function of Leukocytes and Their Interaction With Endothelial Cells.

Science.gov (United States)

Michalak, Barbara; Filipek, Agnieszka; Chomicki, Piotr; Pyza, Małgorzata; Woźniak, Marta; Żyżyńska-Granica, Barbara; Piwowarski, Jakub P; Kicel, Agnieszka; Olszewska, Monika A; Kiss, Anna K

2018-01-01

Aim of the study: Taking into account that overactivated leukocytes are an important factor in the development of many chronic diseases, we investigated the activity of phytochemically characterized (HPLC-DAD-MS n ) extracts from forsythia leaves and flowers on the pro- and anti-inflammatory functions of leukocytes (effects on IL-1β, IL-8, TNF-α, and TGFβ release) and their adherence to endothelial cells. Using bio-guided fractionation, we isolated the active compounds and determined their biological activity, and we included the positive control quercetin. Methods: The effect on IL-1β, TNF-α, IL-8, and TGF-α production by leukocytes was measured by enzyme-linked immunosorbent assay (ELISA). The surface expression of adhesion molecules was analyzed with flow cytometry, and the neutrophil attachment to the endothelial cells was assessed fluorimetrically. The effects on p38MAPK, ERK1/2 and JNK phosphorylation were determined using western blots. Results: Leaf extracts had the effect of decreasing TNF-α production in neutrophils and monocyte/macrophage cells. The bio-guided fractionation led to the isolation of the following lignan aglycones: (+)-pinoresinol, (+)-epipinoresinol, (-)-matairesinol, (+)-phillygenin, and (-)-arctigenin. Only phillygenin was able to stimulate the anti-inflammatory function of macrophages by inducing TGF-β release and IL-10 receptor surface expression. Arctigenin, phillygenin, and a metabolite produced by the gut microbiota, enterolactone, decreased TNF-α and IL-1β production and neutrophil adhesion to endothelial cells, probably by attenuating the p38 and ERK kinase pathways. Conclusion: Forsythia x intermedia is a valuable source of active lignans, which may be potential candidates for treating inflammatory diseases that are associated with the excessive production of cytokines such as TNF-α and IL-1β.

Anuncios y estrategias de campaña en televisión: un balance de la elección intermedia de 2015 en México / Television Campaign Ads and Strategies: The Case of Mexico’s 2015 Mid-term Elections

Directory of Open Access Journals (Sweden)

Oniel Francisco Díaz Jiménez

2016-07-01

Full Text Available En las campañas electorales profesionalizadas, los partidos políticos y los candidatos presentan sus propuestas ante el electorado y buscan maximizar votos, principalmente, a través de los medios de comunicación. En elecciones presidenciales, las estrategias y las tácticas de campaña se enfocan en las imágenes y las propuestas políticas de los candidatos que compiten por la Presidencia. Sin embargo, ¿qué ocurre en elecciones congresionales intermedias, donde los actores principales de la campaña son los partidos políticos y sus candidatos que compiten por cargos legislativos? Este trabajo realiza un análisis de contenido de los anuncios televisivos (spotsde 30 segundos transmitidos por los partidos mexicanos durante las elecciones congresionales intermedias de 2015, con el fin de identificar sus estrategias de campaña. Los resultados muestran diferencias relevantes entre las campañas realizadas por las organizaciones nacionales de los partidos y las campañas de los candidatos individuales a nivel distrital. Sin embargo, también ponen a la vista que los anuncios de ataque fueron un componente central en las estrategias mediáticas de los partidos y los candidatos durante la campaña. / In professionalized election campaigns, political parties and candidates present their policy proposals to the electorate and seek to maximize votes, primarily through media appeals. In presidential elections, campaign tactics and strategies focus on the images and policy proposals of presidential candidates. However, what happens in congressional mid-term elections, where the main campaigners are the political parties and their congressional candidates? This article analyzes the content of all television announcements (30-second spots broadcast by Mexican political parties during the 2015 mid-term congressional elections, in order to identify their campaign strategies. The results show relevant differences between the campaigns run by national

Calibración in situ del sensor cosmos para determinar humedad del suelo en escalas intermedias (~1 km

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Fidencio Cruz Bautista

2013-10-01

Full Text Available La heterogeneidad del suelo influye ampliamente en el contenido de humedad, dificultando la precisa determinación de este parámetro en estudios con fines hidrológicos y ecológicos que requieren de mediciones continuas y representativas para escalas intermedias (~1 km. En este contexto un sensor de neutrón de rayo cósmico The COsmic-ray Soil Moisture Observing System (COSMOS permite cuantificar humedad del suelo de manera continua y a escalas espaciales de cientos de metros. El objetivo de este estudio fue evaluar un esquema de calibración para un sensor COSMOS CRS-1000. El estudio se realizó en una sabana de zacate buffel (Pennisetum ciliare en Rayón Sonora, México. En este sitio se instaló el COSMOS CRS-1000 y para su calibración se realizaron muestreos de suelo en dos etapas. A estas muestras se les determinó el contenido de humedad y su densidad aparente por técnicas gravimétricas. Con el contenido de humedad de estas muestras, expresado en términos volumétricos, se obtuvo por aproximación el parámetro de calibración para el COSMOS CRS-1000. El valor obtenido para este parámetro fue de 4121 conteos por hora (tasa de conteo del neutrón sobre suelo. Con este valor se realizó la corrección a los valores estimados originalmente por el sensor COSMOS CRS-1000. Al realizar esta corrección, se observó un incremento en el contenido de humedad del suelo de 1 a 2 % con respecto a los valores estimados con el COSMOS CRS-1000 en todo el periodo de análisis. A pesar de la variabilidad espacial en el contenido de humedad del suelo bajo estudio, se observó que el sensor COSMOS CRS-1000 tiene la capacidad de proveer estimaciones razonables del contenido de la humedad del suelo de manera continua a una profundidad de 0 a 40 cm, en una superficie de alrededor de 30 ha.

NCBI nr-aa BLAST: CBRC-MDOM-01-0302 [SEVENS

Lifescience Database Archive (English)

Full Text Available CBRC-MDOM-01-0302 ref|ZP_05500348.1| RarD protein, DMT superfamily transporter [Thiomonas intermedia... K12] gb|EEU54627.1| RarD protein, DMT superfamily transporter [Thiomonas intermedia K12] ZP_05500348.1 1.9 27% ...

Tants - üks viis edastada kehalist kogemist / Evelin Lagle

Index Scriptorium Estoniae

Lagle, Evelin, 1986-

2008-01-01

Helsingi teatriakadeemia tantsukompanii TADaC Kumus 25. III 2008: Ervi Siren "Seize the Moment - Snatch, Catch, Enjoy", Jyrki Karttunen "Dramatic Miniatures", Maria Saivosalmi ja Andrius Katinas "Farewell Anna Karenina", Jenni Kivelä "Scrapbook for Rainy Days", Ari Tenhula videomäng/installatsioon "Entr'acte"

Global Positioning System Navigation Algorithms

Science.gov (United States)

1977-05-01

Historical Remarks on Navigation In Greek mythology , Odysseus sailed safely by the Sirens only to encounter the monsters Scylla and Charybdis...TNED 000 00 1(.7 BIBLIOGRAPHY 1. Pinsent, John. Greek Mythology . Paul Hamlyn, London, 1969. 2. Kline, Morris. Mathematical Thought from Ancient to

Moraea intermedia and M. vuvuzela (Iridaceae-Iridoideae, two new species from western South Africa, and some nomenclatural changes and range extensions in the genus

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P. Goldblatt

2010-07-01

Full Text Available We describe two new species in the largely sub-Saharan genus Moraea Mill. (± 205 spp. from its centre of diversity in the winter rainfall region of southern Africa. Moraea intermedia, from north-central Namaqualand near Springbok, is a member of the small section Tubiflorae (now eight species, remarkable in its growth habit with a long basal intemode. leaves clustered at the first aerial node, and Moraea-type stamens and style branches but subequal tepals with very short claws that clasp only the base of the filament column. Moraea vuvuzela. a member of series Galaxia of the Galaxia group of the genus (now 17 species, has deeply fringed stigma lobes, filaments free in the upper 1 mm, ± prostrate, lanceolate leaves and. remarkable for the series, dark brown to purple markings near the base of the tepal limbs. In the unusually variable M.fugax, currently with two subspecies, new collections of subsp. fugax co-occurring but on different soils with subsp.  filicaulis, cast doubt on their current treatment as members of the same species. We now favour recognition of the diminutive subsp.filicaulis as a separate species, M. filicaulis. In the M iripetala group we recommend recognition of the early blooming M. punctata, described in 1892 and later subsumed in M. iripetala but readily distinguished by the long inner  tepals broader in the midline and short, relatively broad, plane rather than channelled leaves. We also report small but significant range extensions for M. barkerae, M. macrocarpa and M. tricolor.

Sense of Threat as a Mediator of Peritraumatic Stress Symptom Development During Wartime: An Experience Sampling Study.

Science.gov (United States)

Lapid Pickman, Liron; Greene, Talya; Gelkopf, Marc

2017-08-01

Exposure and sense of threat have been associated with stress symptoms, yet these relationships have not been clarified during the peritraumatic period. We investigated the mediating role of sense of threat in the link between exposure to rocket warning sirens and stress symptoms during wartime, and the effect of severe mental illness (SMI) status and gender on this mediation. A 30-day twice-daily smartphone-based intensive assessment of exposure to sirens, sense of threat, and peritraumatic stress symptoms was performed during the 2014 Israel-Gaza conflict. Participants included 182 highly exposed individuals with or without SMI. Multilevel structural equation modeling analysis was performed, with SMI status and gender as confounders. Exposure affected the level of peritraumatic stress symptoms both directly, b = 1.07, p development during the peritraumatic timeframe. Intervention and prevention efforts should start early and focus on promoting a sense of safety, particularly with people with SMI. Copyright © 2017 International Society for Traumatic Stress Studies.

Systems to prevent nuclear material from re-entering the biosphere

International Nuclear Information System (INIS)

Buden, D.; Angelo, J.A. Jr.; Lapin, S.

1992-01-01

Nuclear systems are key to the success of many space missions as we have witness in the Apollo science packages, Viking Mars landers, and Pioneer and Voyager planetary exploration missions. There is always a concern that nuclear materials will re-enter the biosphere from a mission abort. In fact, this has happened for radioisotope and reactor power systems. Until now, the emphasize has been an incorporating on-board means to protect the biosphere. With possible increased use of nuclear power and propulsion systems in space, Project SIREN (Search, Intercept, Retrieve, Expulsion, Nuclear) has determined that external means can be used as a back up to current on-board systems to provide assured prevention of nuclear materials from re-entry once in space. The technology base to implement a SIREN vehicle has been assessed and a data base and mission analysis program prepared (called THOR) to evaluate various missions. The degree of hazard from existing nuclear power systems in space has been assessed and found to be significant

Excellent and durable response to radiotherapy in a rare case of spinal cord compression due to extra-medullary hematopoiesis in β-thalassemia intermedia: case report and clinicoradiological correlation.

Science.gov (United States)

Yathiraj, Prahlad H; Singh, Anshul; Vidyasagar, Sudha; Varma, Muralidhar; Mamidipudi, Vidyasagar

2017-04-01

Spinal cord compression (SCC) is an unusual sequale of extra-medullary hematopoiesis (EMH). We report a patient diagnosed with β-thalassemia intermedia at the age of 7 years presenting as a 24-year-old with symptoms suggestive of paraparesis. MR imaging revealed long masses of EMH opposite T5-T11 and L5-S2 vertebrae with cord compression at T6 vertebrae. Patient was treated with external beam radiotherapy (EBRT) to a low dose of 20 Gy in 10 fractions over 2 weeks. The patient had symptomatic relief of paraparesis by the 5th fraction and nearly regained full power in bilateral lower limbs by EBRT conclusion. Patient was begun on hydroxyurea post EBRT and was symptom free at 2-month follow up. With a follow-up of 18 months so far, he remains asymptomatic and free of recurrence. MRI correlation of pre-EBRT, post-EBRT and at first follow-up showed a significant reduction in the size of EMH, increase in diameter of spinal canal post EBRT but a persistent edema which had no clinical manifestation. Though there was a 58% drop in leukocyte count by the end of EBRT, there was no leukocytopenia. We suggest that EBRT should be treatment of choice for SCC due to EMH as it produces as rapid and durable response with minimal acute hematological side-effects.

Colleges Grapple with the "Behavioral Broken Arm"

Science.gov (United States)

Hoover, Eric

2008-01-01

After the fatal shootings at Virginia Tech last April, colleges went shopping for hardware. They bought sirens, mass-messaging systems, surveillance cameras, and door locks. Some colleges armed their police departments for the first time. Others added assault rifles to their arsenals. "Active shooter" drills happened everywhere. As administrators…

Stroke Investigative Research and Education Network: Community Engagement and Outreach within Phenomics Core

Science.gov (United States)

Jenkins, Carolyn; Arulogun, Oyedunni Sola; Singh, Arti; Mande, Aliyu T.; Ajayi, Eric; Benedict, Calys Tagoe; Ovbiagele, Bruce; Lackland, Daniel T.; Sarfo, Fred Stephen; Akinyemi, Rufus; Akpalu, Albert; Obiako, Reginald; Melikam, Enzinne Sylvia; Laryea, Ruth; Shidali, Vincent; Sagoe, Kwamena; Ibinaiye, Philip; Fakunle, Adekunie Gregory; Owolabi, Lukman F.; Owolabi, Mayowa O.

2016-01-01

Stroke is the leading cause of neurological hospital admissions in sub-Saharan Africa (SSA) and the second leading cause of death globally. The Stroke Investigative Research and Education Network [SIREN] seeks to comprehensively characterize the genomic, sociocultural, economic, and behavioral risk factors for stroke and to build effective teams…

Evaluation of cytotoxic effects and acute and chronic toxicity of aqueous extract of the seeds of Calycotome villosa (Poiret) Link (subsp. intermedia) in rodents.

Science.gov (United States)

Lyoussi, Badiaa; Cherkaoui Tangi, Khadija; Morel, Nicole; Haddad, Mohamed; Quetin-Leclercq, Joelle

2018-01-01

The present investigation was carried out to evaluate the safety of an aqueous extract of the seeds of Calycotome villosa (Poiret) Link (subsp. intermedia) by determining its cytotoxicity and potential toxicity after acute and sub-chronic administration in rodents. Cytotoxic activity was tested in cancer and non-cancer cell lines HeLa, Mel-5, HL-60 and 3T3. Acute toxicity tests were carried out in mice by a single oral administration of Calycotome seed-extract (0 - 12 g/kg) as well as intraperitoneal doses of 0 - 5 g/kg. Sub-chronic studies were conducted in Wistar rats by administration of oral daily doses for up to 90 days. Changes in body and vital organ weights, mortality, haematology, clinical biochemistry and histologic morphology were evaluated. The lyophilized aqueous extract of C. villosa exhibited a low cytotoxicity in all cell lines tested with an IC 50 > 100 µg/ml. In the acute study in mice, intra-peritoneal administration caused dose-dependent adverse effects and mortality with an LD 50 of 4.06 ± 0.01 g/kg. In the chronic tests, neither mortality nor visible signs of lethality was seen in rats. Even AST and ALT were not affected while a significant decrease in serum glucose levels, at 300 and 600 mg/kg was detected. Histopathological examination of the kidney and liver did not show any alteration or inflammation at the end of treatment. In conclusion, the aqueous extract of C. villosa seed appeared to be non-toxic and did not produce mortality or clinically significant changes in the haematological and biochemical parameters in rats.

Leishmaniose tegumentar americana na Ilha Grande. Rio de Janeiro: V. Observações sobre a biologia dos transmissores em condiçoes naturais

Directory of Open Access Journals (Sweden)

Nelson A. de Araújo Filho

1981-12-01

Full Text Available Durante um surto de Leishmaniose Tegumentar Americana (LTA na Praia Vermelha, Ilha Grande, município de Angra dos Reis, Rio de Janeiro, foram coletados 4.192 exemplares de flebotomíneos em ambiente domiciliar e silvestre, com isca animal e outros abrigos naturais, durante o período de janeiro de 1976 a abril de 1977. Entre o total de 11 espécies coletadas encontraram-se 2.493 exemplares de Lutzomyia intermedia, 1.594 de Lutzomyia migonei e 105 exemplares de outras espécies. A L. intermedia apresentou densidade mais elevada no domicílio humano, enquanto a L. migonei apresentou coletas mais rendosas com iscas animais e galinheiros. O sinantropismo e a elevada densidade no ambiente domiciliar incriminam as espécies L. intermedia e L. migonei como as prováveis vetoras da LTA na Ilha Grande.During an outbreak of mucocutaneous leishmaniasis in Praia Vermelha, Ilha Grande (Great Island in the county of Angra dos Reis, State of Rio de Janeiro, 4.192 species of Phlebotomus were collected in domestic and wild environment with the use of animal baits and other natural shelters from January 1976 to April 1977. In a total of 11 species collected, 2.493 samples of Lutzomyia intermedia, 1.594 of Lutzomyia migonei and 105 samples of other species were found. The Lutzomyia intermedia presented a higher density in human dwellings, while the Lutzomyia migonei presented better results with animal baits and chicken-coops. The sinantropism and high density found in the houses incriminate the species L. intermedia and L. migonei as the probable vectors of Mucocutaneous Leishmaniasis in Ilha Grande.

Phlebotomines (Diptera, Psychodidae in the Speleological Province of the Ribeira Valley: 3. Serra district - area of hostels for tourists who visit the Parque Estadual do Alto Ribeira (PETAR, state of São Paulo, Brazil

Directory of Open Access Journals (Sweden)

Eunice Aparecida Bianchi Galati

2010-01-01

Full Text Available Phlebotomines (Diptera, Psychodidae in the Speleological Province of the Ribeira Valley: 3. Area of hostels for tourists who visit the Parque Estadual do Alto Ribeira (PETAR, state of São Paulo, Brazil. The study characterizes some ecological aspects of the phlebotomine fauna in an endemic area of cutaneous leishmaniasis (CL situated in the Serra district, Iporanga municipality where the hostels for tourists visiting the PETAR are located. Captures were undertaken on a smallholding and a small farm situated near the hostels, monthly between January/2001 and December/2003 with automatic light traps (ALT in pigsty, hen-house and veranda of a domicile at the two sites, and in peridomicile of the small farm also with black/white Shannon traps. With the ALT a total of 87,224 phlebotomines representing 19 species and also two hybrids of Nyssomyia intermedia (Lutz & Neiva and Nyssomyia neivai (Pinto and two anomalous specimens were captured. The standardized index species abundance was for Ny. intermedia = 1.0 and Ny. neivai = 0.935. The highest frequencies of the smallholding occurred in the pigsty, the Williams' mean/capture for Ny. intermedia being 63.7 specimens and for Ny. neivai 29.2, and on the small farm, in the hen-house, Ny. intermedia 402.6 and Ny. neivai 116.2. A total of 863 phlebotomines (Ny. intermedia: 75.4%; Ny. neivai: 24.3% were captured with black/white Shannon traps; females of both species being predominant in the white trap. The high frequencies of Ny. intermedia and Ny. neivai, both implicated in CL transmission, indicate the areas presenting risk of the disease.

Verdes Mares: The Ideology of the Siren

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Bruno Marinoni Marinoni

2009-02-01

Full Text Available The television channel number 10 of Fortaleza (current TV Verdes Mares, during the 1960 decade, passed through the ideological filter of the Military Dictatorship, when the channel was taken from its owner and given to the businessman Edson Queiroz. The filter had consequences in the next decade with the channel affiliation to the biggest network broadcasting of the country, the Rede Globo, which was also protected by the Military Regime. Based on those two facts, it is intended to discuss some aspects of the exploration models of communication that occurs in the country since the specified period, emphasizing the ideology transmitted by the TV channel which has the biggest audience ratings in Ceará.

33 CFR 165.1187 - Security Zones; Golden Gate Bridge and the San Francisco-Oakland Bay Bridge, San Francisco Bay...

Science.gov (United States)

2010-07-01

... Limited Access Areas Eleventh Coast Guard District § 165.1187 Security Zones; Golden Gate Bridge and the... Golden Gate Bridge and the San Francisco-Oakland Bay Bridge, in San Francisco Bay, California. (b... siren, radio, flashing light, or other means, the operator of a vessel shall proceed as directed. [COTP...

Mixing Under Transcritical Flow Conditions

Science.gov (United States)

2011-03-01

between them. The signals were then sent to two amplifiers (Krohn-Hite model 7500 and a Trek model PZD2000A), one for each piezo-siren. The amplified...In Annual Research Briefs, pages 73–84. Center for Turbulence Research, NASA Ames/Stanford Univ., 1999. [61] C. Segal and SA Polikhov. Subcritical to

In vitro cytokine responses to periodontal pathogens: generalized aggressive periodontitis is associated with increased IL-6 response to Porphyromonas gingivalis

DEFF Research Database (Denmark)

Borch, T S; Holmstrup, Palle; Bendtzen, K

2010-01-01

with GAgP and 10 controls stimulated with periodontal pathogens or a control antigen, tetanus toxoid (TT) in the presence of autologous serum. The pathogens used were Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum, either as type strains or bacteria isolated from...... the participants' inherent oral flora. The P. gingivalis -induced production of IL-6 was approximately 2.5-fold higher in patients with GAgP than in healthy controls (P gingivalis, as all cytokine...... responses induced by Pr. intermedia, F. nucleatum and TT was similar in the two groups. A reduced IL-12p70 response to Pr. intermedia and F. nucleatum was observed in smokers compared to non-smoking patients (P gingivalis, MNC...

Setting the media agenda: A study of the 2010 Sudanese presidential elections

Directory of Open Access Journals (Sweden)

Syed Arabi Idid

2015-06-01

Full Text Available This research investigates the influence of new media agendas on traditional news media according to the theoretical framework of intermedia agenda-setting during the 2010 Sudanese presidential elections. Upon applying content analysis, cross-lagged correlation analysis, and Rozelle-Campbell Baseline analysis, the paper provides evidence of intermedia agenda-setting in Sudanese media across a number of dependent variables. Sudanese newspapers influenced the agenda of Sudanese blogs whilst blogs have an increasing impact on the agenda of the newspapers. Our content analysis found that the agendas of socio-political blogs were strongly correlated with those of the newspapers. The findings also presented evidence of intermedia agenda-setting between socio-political blogs and citizen journalism.

Who is to change all that

International Nuclear Information System (INIS)

Huber, J.

1980-01-01

How practical is the practice of the alternative movement. The illusion of alternative economics. Simply more and more. The internal limits of the projects. How far can the alternative movement stabilize the system. Two cultures - no thanks. Established subculturals and intermedias. The alternative movement and the development of industrial society. Criteria for intermediaer projects. (orig.) [de

Project B610 process control configuration acceptance test procedure

International Nuclear Information System (INIS)

Silvan, G.R.

1994-01-01

The purpose of this test is to verify the Westinghouse configuration of the MICON A/S Distributed Control System for project B610. The following will be verified: proper assignment and operation of all field inputs to and outputs from the MICON Termination panels; proper operation of all display data on the operator's console; proper operation of all required alarms; and proper operation of all required interlocks. The MICON A/S control system is configured to replace all the control, indication, and alarm panels now located in the Power Control Room. Nine systems are covered by this control configuration, 2736-ZB HVAC, 234-5Z HVAC, Process Vacuum, Dry Air, 291-Z Closed Loop Cooling, Building Accelerometer, Evacuation Siren, Stack CAMs, and Fire. The 2736-ZB HVAC system consists of the ventilation controls for 2736-ZB and 2736-Z as well as alarms for the emergency generators and 232-Z. The 234-5Z HVAC system is the ventilation controls for 235-5Z and 236-Z buildings. Process Vacuum covers the controls for the 26 inch vacuum system. Dry Air covers the controls for the steam and electric air dryers. The 291-Z Closed Loop Cooling system consists of the status indications and alarms for the 291-Z compressor and vacuum pump closed loop cooling system. The rest of closed loop cooling was tested earlier. The Building Accelerometer system consists of the status indications for the two seismic system accelerometers. The Evacuation Siren system includes the controls for the evacuation and take cover sirens. Stack CAMs cover the alarms for the various building ventilation stack continuous air monitors. Finally, the Fire system covers the various fire alarms now located in Room 321-A

Health Plan Performance Measurement within Medicare Subvention.

Science.gov (United States)

1998-06-01

the causes of poor performance (Siren & Laffel, 1996). Although outcomes measures such as nosocomial infection rates, admission rates for select...defined. Traditional outcomes measures include infection rates, morbidity, and mortality. The problem with these traditional measures is... Maternal /Child Care Indicators Nursing Staffing Indicators Outcome Indicators Technical Outcomes Plan Performance Stability of Health Plan

Intermedia and Intermittency

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Veres Bálint

2014-12-01

Full Text Available It is commonly known that medial reflections have been initiated by attempts to secure the borders of discrete medial forms and to define the modus operandi of each essentialized medial area. Later on, the focus of study has shifted to plurimedial formations and the interactions between predefined medial genres. In the last few decades, taxonomic approaches to various multi-, inter-, and transmedial phenomena dominated the discussions, which offered invaluable support in mapping the terrain, but at the same time hindered the analysis of the ephemeral, time-dependent aspects of plurimedial operations. While we explore the properties of each medial configuration, we lose sight of the actual historical drivers that produce ever-new configurations. My thesis is that any discourse on intermediality should be paralleled by a discourse on cultural intermittency, and consequently, media studies should involve an approach that focuses on the “ecosystem” of the constantly renewing media configurations from the point of view of their vitalizing potential and capability to trigger heightened experiences. This approach draws much inspiration from K. Ludwig Pfeiffer’s media anthropology that gives orientation in my paper.

Techniques for Soundscape Retrieval and Synthesis

Science.gov (United States)

Mechtley, Brandon Michael

The study of acoustic ecology is concerned with the manner in which life interacts with its environment as mediated through sound. As such, a central focus is that of the soundscape: the acoustic environment as perceived by a listener. This dissertation examines the application of several computational tools in the realms of digital signal processing, multimedia information retrieval, and computer music synthesis to the analysis of the soundscape. Namely, these tools include a) an open source software library, Sirens, which can be used for the segmentation of long environmental field recordings into individual sonic events and compare these events in terms of acoustic content, b) a graph-based retrieval system that can use these measures of acoustic similarity and measures of semantic similarity using the lexical database WordNet to perform both text-based retrieval and automatic annotation of environmental sounds, and c) new techniques for the dynamic, realtime parametric morphing of multiple field recordings, informed by the geographic paths along which they were recorded.

Financing the alternative: renewable energy in developing and transition countries

OpenAIRE

Brunnschweiler, Christa N.

2006-01-01

This paper examines the determinants of credit allocation to renewable energy firms in developing and transition countries. Using a simple en- dogenous growth model, we show that the development of the renewable energy sector, i.e. the diversification of renewable energy resources used in primary energy production, depends on the quality of financial intermedia- tion, debtor information costs to banks, and financing needs of renewable energy firms. Policies should aim at increasing financial ...

VERIFICACIÓN DEL CONTENIDO DE MATERIA SECA COMO INDICADOR DE COSECHA PARA AGUACATE (persea americana CULTIVAR HASS EN ZONA INTERMEDIA DE PRODUCCIÓN DE LOS SANTOS, COSTA RICA

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María del Milagro Cerdas Araya

2014-01-01

Full Text Available El objetivo de esta investigación fue estu - diar si el contenido de materia seca (CMS de aguacate podría ser utilizado como un índice de cosecha adecuado en sustitución del contenido de aceite (CA en aguacate Hass, cultivado en una zona de altitud intermedia (1750 msnm, en Los Santos, Costa Rica. Se marcaron 100 árboles y un total de 2000 flores polinizadas; se evaluaron 35 frutos cada semana, de la semana 31 a la 44. Se encontró que ambos, CA y CMS, mostraron un aumento rápido y significativo después de la semana 39 (p≥0,05, con un coeficiente de correlación entre ellos de 0,92. Otros cambios observados simultáneamente, y relacionados con la maduración de la fruta, fueron el desarrollo del color morado en la cáscara (74% de la superficie, la reducción de la adherencia de la cubierta de la semilla a la pulpa de la fruta, y de ésta a la cáscara y a la semilla, así como la disminución de la firmeza del fruto, de 118 N durante la semana 31 hasta 57 N en la semana 40 y después de ésta. Se recomienda usar el 23% de CMS en el fruto de aguacate Hass como un índice de cosecha para el aguacate cultivado a 1750 msnm en la zona de Los Santos, como una alternativa al conteni - do de aceite de la fruta (CA.

Chemical Compounds and Bioactivity of Aqueous Extracts of Alibertia spp. in the Control of Plutella xylostella L. (Lepidoptera: Plutellidae).

Science.gov (United States)

Peres, Lucas L S; Sobreiro, Ana I; Couto, Irys F S; Silva, Rosicléia M; Pereira, Fabricio F; Heredia-Vieira, Silvia C; Cardoso, Claudia A L; Mauad, Munir; Scalon, Silvana P Q; Verza, Sandra S; Mussury, Rosilda M

2017-11-22

Successive applications of insecticides to control Plutella xylostella L. (Lepidoptera: Plutellidae) have resulted in the emergence of resistant populations of this insect. A novel control measure for this target insect could be the use of botanical insecticides derived from plant tissues. Hence, we experimentally tested aqueous extracts of Alibertia edulis (Rich.), Alibertia intermedia (Mart.), and Alibertia sessilis (Vell.) K. Schum. found in the Brazilian savannah in order to investigate their potential to disrupt the life cycle of P. xylostella . Aqueous extracts of the leaves of A. intermedia and A. sessilis negatively affected the development of P. xylostella in all stages of the life cycle, prolonging the larval stage and causing mortality in the larval or pupal stages. Treatments with A. intermedia and A. sessilis extracts caused the lowest fecundity and the number of hatched larvae. The harmful effects of these aqueous extracts on the life cycle of P. xylostella may be attributable to the flavonoids and other phenolic compounds present in A. intermedia and A. sessilis . These aqueous botanical extracts are low in toxicity when compared to non-aqueous pesticides, and may emerge as an effective approach for control of populations of P. xylostella .

Chemical Compounds and Bioactivity of Aqueous Extracts of Alibertia spp. in the Control of Plutella xylostella L. (Lepidoptera: Plutellidae

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Lucas L. S. Peres

2017-11-01

Full Text Available Successive applications of insecticides to control Plutella xylostella L. (Lepidoptera: Plutellidae have resulted in the emergence of resistant populations of this insect. A novel control measure for this target insect could be the use of botanical insecticides derived from plant tissues. Hence, we experimentally tested aqueous extracts of Alibertia edulis (Rich., Alibertia intermedia (Mart., and Alibertia sessilis (Vell. K. Schum. found in the Brazilian savannah in order to investigate their potential to disrupt the life cycle of P. xylostella. Aqueous extracts of the leaves of A. intermedia and A. sessilis negatively affected the development of P. xylostella in all stages of the life cycle, prolonging the larval stage and causing mortality in the larval or pupal stages. Treatments with A. intermedia and A. sessilis extracts caused the lowest fecundity and the number of hatched larvae. The harmful effects of these aqueous extracts on the life cycle of P. xylostella may be attributable to the flavonoids and other phenolic compounds present in A. intermedia and A. sessilis. These aqueous botanical extracts are low in toxicity when compared to non-aqueous pesticides, and may emerge as an effective approach for control of populations of P. xylostella.

Activation of human gingival epithelial cells by cell-surface components of black-pigmented bacteria: augmentation of production of interleukin-8, granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor and expression of intercellular adhesion molecule 1.

Science.gov (United States)

Sugiyama, A; Uehara, A; Iki, K; Matsushita, K; Nakamura, R; Ogawa, T; Sugawara, S; Takada, H

2002-01-01

Black-pigmented anaerobic bacteria, such as Porphyromonas gingivalis and Prevotella intermedia, are amongst the predominant bacteria in periodontal pockets and have been implicated in periodontal diseases. To elucidate the roles of gingival keratinocytes, which are the first cells encountered by oral bacteria in periodontal diseases, human gingival keratinocytes in primary culture were stimulated with cell-surface components of P gingivalis and Pr. intermedia. A glycoprotein fraction from Pr. intermedia (PGP) clearly augmented the release of interleukin-8, granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor, as determined by enzyme-linked immunosorbent assay. This PGP also induced expression of intercellular adhesion molecule-1 (ICAM-1), as determined by flow cytometry. The augmentation of mRNA expression for these molecules was also confirmed by reverse transcription PCR. In contrast, lipopolysaccharide (LPS) from Pr. intermedia and Escherichia coli was completely inactive in these assays. LPS fraction and purified fimbriae from P gingivalis exhibited weak activities. Cytokine production and ICAM-1 expression by gingival keratinocytes might cause accumulation and activation of neutrophils in the epithelium and, therefore, may be involved in the initiation and development of inflammation in periodontal tissues.

Adipocytokine concentrations in children with different types of beta-thalassemia.

Science.gov (United States)

Enli, Yaşar; Balci, Yasemin I; Gönen, Cafer; Uzun, Ebru; Polat, Aziz

2014-06-01

Beta-thalassemia is an inherited blood disorder. It results from the impaired production of β-globin chains, leading to a relative excess of alpha-globin chains. Clinical severity separates this disease into three main subtypes: β- thalassemia major, β-thalassemia intermedia and β-thalassemia minor, the former two being clinically more significant. Inflammatory processes may play an important role in some of the complications of thalassemia. Adipose tissue is one of the most important endocrine and secretory organs that release adipocytokines like adiponectin, resistin and visfatin. The aim of our study was to analyze adipocytokine concentrations (adiponectin, resistin and visfatin) in different types of β-thalassemia patients and determine any possible correlations with disease severity. We recruited 29 patients who were transfusion-dependent β-thalassemia-major patients, 17 patients with β-thalassemia intermedia, 30 β-thalassemia minor patients. The control group consisted of 30 healthy children. Anthropometric measurements, complete blood count, biochemical parameters, serum concentrations of adiponectin, resistin, visfatin were performed for all subjects. Resistin and visfatin concentrations were significantly higher in β-thalassemia minor patients than in controls. Adiponetin, resistin and visfatin concentrations were significantly higher in both β-thalassemia intermedia and major patients than in controls. The concentrations of adiponectin, resistin and visfatin were significantly higher in both β-thalassemia intermedia and major patients than in β-thalassemia minor patients. There was no significant difference between β-thalassemia intermedia and β-thalassemia major patients for adipocytokines concentrations. We speculate that these adipocytokines may play a role in the development of complications in β-thalassaemia.

Profiling of Human Acquired Immunity Against the Salivary Proteins of Phlebotomus papatasi Reveals Clusters of Differential Immunoreactivity

Science.gov (United States)

2014-03-10

leishmaniasis.56 Pre-exposure of PROFILING OF SAND FLY SALIVARY PROTEINS 935 murine cells to L. intermedia salivary sonicates resulted in decreased IP-10...Thompson JD, Higgins DG, 2011. Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol 7...Brodskyn C, Barral A, de Oliveira CI, 2010. Immunity to Lutzomyia intermedia saliva modulates the inflammatory environ- ment induced by Leishmania

Scanning electron microscopy and fluorescent in situ hybridization of experimental Brachyspira (Serpulina) pilosicoli infection in growing pigs

DEFF Research Database (Denmark)

Jensen, Tim Kåre; Møller, Kristian; Boye, Mette

2000-01-01

Two groups of six 8-week-old pigs were challenged with 1X10(9) cfu Brachyspira (Serpulina) pilosicoli or Serpulina intermedia daily for 3 consecutive days to study the pathology of porcine colonic spirochetosis by scanning electron microscopy (SEM) and fluorescent in situ hybridization (FISH......; however, only two pigs developed transient watery diarrhea. S. intermedia was reisolated from four of the inoculated pigs, but clinical signs were not observed. Gross examination of the B. pilosicoli-infected pigs revealed dilated large intestines with a hyperemic mucosa, whereas the large intestines...... of the S. intermedia-inoculated pigs and the control pigs appeared normal. SEM examination of B. pilosicoli-infected pigs revealed degenerated epithelial cells and spirochetal colonization of the colonic mucosa in four pigs. By FISH, B. pilosicoli cells were found colonizing and invading the surface...

Risk factors for intestinal pathogens in Danish finishing pig herds

DEFF Research Database (Denmark)

Stege, H.; Jensen, Tim Kåre; Møller, Kristian

2001-01-01

The objective of this investigation was to identify risk factors for infection with the intestinal bacteria: Lawsonia intracellularis, Brachyspira hyodysenteriae, Serpulina intermedia, Brachyspira innocens, Brachyspira pilosicoli and swine-pathogenic Escherichia coli (serogroups O138, O139, O141....... intermedia, B. innocens, B. pilosicoli) (OR's = 0.43 and 0.06, respectively). 2. Home-mixed (and/or non-pelleted) feed was associated with reduced prevalences of L. intracellularis and weakly beta -haemolytic spirochetes (OR's = 0.6 and 0.4, respectively). 3. Providing straw to finishers was associated...... with a reduced prevalence of weakly beta -haemolytic spirochetes (OR = 0.28-0.32). 4. Not using antimicrobial growth promoters for piglets was associated with an increased prevalence of S. intermedia (OR = 11.11). 5. Rare occurrence of post-weaning diarrhoea (as opposed to common) was associated...

USSR Report: Political and Sociological Affairs, No. 1478.

Science.gov (United States)

1983-11-18

34 [indyuk], " whale " [Mt], "gooseberry" [kryzhovnik], "palm tree" [pal’ma], "lilac" [siren’], "battle" [srazheniye], and "navy" [flot]. You have to... addict , then on a swindling salesman, then on a degenerate priest who has just come from the bed of his lover. Adding that, "all of them are

Alpha Centauri's siren call has frustrated planet hunters

Science.gov (United States)

Clery, Daniel

2018-04-01

Alpha Centauri, a three-star system just 4 light-years away that is the sun's nearest neighbor, ought to be a great place to look for Earth-like planets. But last week, at a meeting of the European Astronomical Society here, astronomers lamented that the system has so far thwarted discovery efforts—and announced new schemes to probe it. The two sunlike stars, Alpha Centauri A and B, orbit each other closely while Proxima Centauri, a tempestuous red dwarf, hangs onto the system tenuously in a much more distant orbit. In 2016, astronomers discovered an Earth-mass planet around Proxima Centauri, but few think the planet, blasted by radiation and fierce stellar winds, is habitable. Astrobiologists believe the other two stars are more likely to host temperate, Earth-like worlds.

Rapid Identification of Key Pathogens in Wound Infection by Molecular Means

Science.gov (United States)

2006-01-01

3 3 5 4 7 T I NT NT NT NT - - - Prevotella denticola ATCC 33185 1 NT NT NT NT - - - Prevotella intermedia ATCC 2561 IT I NT NT NT NT - - Prevotella...TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia , tetQ gene and total bacteria...detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. J. Clin.Microbiol. 41, 863-866. (2003). 20. Higgins ,D. et al. CLUSTAL W

Impact of early colonizers on in vitro subgingival biofilm formation.

Directory of Open Access Journals (Sweden)

Thomas W Ammann

Full Text Available The aim of this study was to investigate the impact of early colonizing species on the structure and the composition of the bacterial community developing in a subgingival 10-species biofilm model system. The model included Streptococcus oralis, Streptococcus anginosus, Actinomycesoris, Fusobacterium nucleatum subsp. nucleatum, Veillonella dispar, Campylobacter rectus, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. Based on literature, we considered Streptococcus oralis, Streptococcus anginosus, and Actinomyces oris as early colonizers and examined their role in the biofilms by either a delayed addition to the consortium, or by not inoculating at all the biofilms with these species. We quantitatively evaluated the resulting biofilms by real-time quantitative PCR and further compared the structures using confocal laser scanning microscopy following fluorescence in situ hybridisation. The absence of the early colonizers did not hinder biofilm formation. The biofilms reached the same total counts and developed to normal thickness. However, quantitative shifts in the abundances of individual species were observed. In the absence of streptococci, the overall biofilm structure appeared looser and more dispersed. Moreover, besides a significant increase of P. intermedia and a decrease of P. gingivalis , P. intermedia appeared to form filamented long chains that resembled streptococci. A. oris, although growing to significantly higher abundance in absence of streptococci, did not have a visible impact on the biofilms. Hence, in the absence of the early colonizers, there is a pronounced effect on P. intermedia and P. gingivalis that may cause distinct shifts in the structure of the biofilm. Streptococci possibly facilitate the establishment of P. gingivalis into subgingival biofilms, while in their absence P. intermedia became more dominant and forms elongated chains.

Subgingival bacteria in Ghanaian adolescents with or without progression of attachment loss

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Gunnar Dahlén

2014-05-01

Full Text Available Objective: This study describes subgingival bacterial profiles associated with clinical periodontal status in Ghanaian adolescents with or without progression of attachment loss. Materials and methods: Among 500 adolescents included in a cohort study, 397 returned 2 years later for a periodontal re-examination, including full-mouth CAL measurements. At follow-up, a subgroup of 98 adolescents was also subjected to bacterial sampling with paper points at four periodontal sites (mesial aspect of 11, 26, 31, and 46 and analyzed with the checkerboard DNA–DNA hybridization technique against DNA-probes from nine periodontitis-associated bacterial species. Results: The 98 Ghanaian adolescents examined in the present study were similar to the entire group examined at the 2-year follow-up with respect to age, gender, and CAL ≥3 mm. A high detection frequency of Fusobacterium nucleatum and Prevotella intermedia (>99% using checkerboard analysis was found, while for Aggregatibacter actinomycetemcomitans the detection frequency was <50%. A strong correlation was found at the individual level between the presence of P. intermedia and the total CAL change, and P. intermedia and Porphyromonas gingivalis were strongly correlated with a change in CAL and probing pocket depth (PPD at the sampled sites. In a linear regression model, a significant discriminating factor for the total CAL change in the dentition during the 2-year follow-up period was obtained for P. intermedia and public school. Conclusion: This study indicates that subgingival bacterial species other than A. actinomycetemcomitans, for example, P. intermedia, have a significant association with periodontal breakdown (change in CAL in Ghanaian adolescents with progression of periodontal attachment loss.

Planning for a space infrastructure for disposal of nuclear space power systems

International Nuclear Information System (INIS)

Angelo, J. Jr.; Albert, T.E.; Lee, J.

1989-01-01

The development of safe, reliable, and compact power systems is vital to humanity's exploration, development, and, ultimately, civilization of space. Nuclear power systems appear to present to offer the only practical option of compact high-power systems. From the very beginning of US space nuclear power activities, safety has been a paramount requirement. Assurance of nuclear safety has included prelaunch ground handling operations, launch, and space operations of nuclear power sources, and more recently serious attention has been given to postoperational disposal of spent or errant nuclear reactor systems. The purpose of this paper is to describe the progress of a project to utilize the capabilities of an evolving space infrastructure for planning for disposal of space nuclear systems. Project SIREN (Search, Intercept, Retrieve, Expulsion - Nuclear) is a project that has been initiated to consider post-operational disposal options for nuclear space power systems. The key finding of Project SIREN was that although no system currently exists to affect the disposal of a nuclear space power system, the requisite technologies for such a system either exist or are planned for part of the evolving space infrastructure

Blood transfusion versus hydroxyurea in beta-thalassemia in Iran: a cost-effectiveness study.

Science.gov (United States)

Ravangard, Ramin; Mirzaei, Zahra; Keshavarz, Khosro; Haghpanah, Sezaneh; Karimi, Mehran

2017-11-21

Thalassemia intermedia is a type of anemia which has several treatments modalities. We aimed to study the cost effectiveness of two treatments, including blood transfusion and hydroxyurea, in patients with beta-thalassemia intermedia in south of Iran referred to a referral center affiliated to Iran, Shiraz University of Medical Sciences in 2015. This was a cost-effectiveness study which was conducted on 122 patients with beta-thalassemia intermedia. The indicator of effectiveness in this study was the reduction of growth disorder (normal BMI). Data analysis was done using SPSS 21, Excel 2010 and Treeage 2011. Finally, the one-way sensitivity analysis was performed to determine the robustness of the results. The average annual costs of blood transfusion and the use of hydroxyurea in 2015 were 20733.27 purchasing power parity (PPP)$ and 7040.29 PPP$, respectively. The effectiveness of blood transfusion was57.4% while in hydroxyurea group was 60.7%. The results showed that the cost effectiveness of using hydroxyurea was more than that of blood transfusion. Therefore, it is recommended that the use of hydroxyurea in the treatment of patients with beta-thalassemia intermedia would become the first priority, and more basic and supplementary insurance coverage for treating such patients using hydroxyurea should be considered.

Louisiana Coastal Area, Louisiana. Freshwater Diversion to Barataria and Breton Sound Basins. Feasibility Study. Volume 2. Technical Appendixes A, B, C, and D.

Science.gov (United States)

1984-09-01

the bullfrog, pig frog, bronze frop, leopard frog, lesser siren, gulf coast toad, preen and squirrel treefrogs, cricket frog, and several species of...geese that winter in significant numbers are lesser snow geese. Other game birds include coots, rails, gallinules, snipe, woodcock, and mourning doves...98 • . - . . . ." . . . . - " - -. - . . Simoneaux, L.F. 1979. The distribution of menhaden, genus Brevortia. with respect to salinity, in the upper

Distance probes of dark energy

Energy Technology Data Exchange (ETDEWEB)

Kim, A. G.; Padmanabhan, N.; Aldering, G.; Allen, S. W.; Baltay, C.; Cahn, R. N.; D’Andrea, C. B.; Dalal, N.; Dawson, K. S.; Denney, K. D.; Eisenstein, D. J.; Finley, D. A.; Freedman, W. L.; Ho, S.; Holz, D. E.; Kasen, D.; Kent, S. M.; Kessler, R.; Kuhlmann, S.; Linder, E. V.; Martini, P.; Nugent, P. E.; Perlmutter, S.; Peterson, B. M.; Riess, A. G.; Rubin, D.; Sako, M.; Suntzeff, N. V.; Suzuki, N.; Thomas, R. C.; Wood-Vasey, W. M.; Woosley, S. E.

2015-03-01

This document presents the results from the Distances subgroup of the Cosmic Frontier Community Planning Study (Snowmass 2013). We summarize the current state of the field as well as future prospects and challenges. In addition to the established probes using Type Ia supernovae and baryon acoustic oscillations, we also consider prospective methods based on clusters, active galactic nuclei, gravitational wave sirens and strong lensing time delays.

Bayesian inference for partially identified models exploring the limits of limited data

CERN Document Server

Gustafson, Paul

2015-01-01

Introduction Identification What Is against Us? What Is for Us? Some Simple Examples of Partially Identified ModelsThe Road Ahead The Structure of Inference in Partially Identified Models Bayesian Inference The Structure of Posterior Distributions in PIMs Computational Strategies Strength of Bayesian Updating, Revisited Posterior MomentsCredible Intervals Evaluating the Worth of Inference Partial Identification versus Model Misspecification The Siren Call of Identification Comp

Merger of two dispatch centres: does it improve quality and patient safety?

Science.gov (United States)

Moser, Alexandre; Mettler, Annette; Fuchs, Vincent; Hanhart, Walter; Robert, Claude-François; Della Santa, Vincent; Dami, Fabrice

2017-04-13

Dispatch centres (DCs) are considered an essential but expensive component of many highly developed healthcare systems. The number of DCs in a country, region, or state is usually based on local history and often related to highly decentralised healthcare systems. Today, current technology (Global Positioning System or Internet access) abolishes the need for closeness between DCs and the population. Switzerland went from 22 DCs in 2006 to 17 today. This study describes from a quality and patient safety point of view the merger of two DCs. The study analysed the performance (over and under-triage) of two medical DCs for 12 months prior to merging and for 12 months again after the merger in 2015. Performance was measured comparing the priority level chosen by dispatcher and the severity of cases assessed by paramedics on site using the National Advisory Committee for Aeronautics (NACA) score. We ruled that NACA score > 3 (injuries/diseases which can possibly lead to deterioration of vital signs) to 7 (lethal injuries/diseases) should require a priority dispatch with lights and siren (L&S). While NACA score  3. Prior to merging, Dispatch A had a sensitivity/specificity regarding the use of lights and sirens and severity of cases of 86%/48% with over- and under-triage rates of 78% and 5%, respectively. Dispatch B had sensitivity and specificity of 92%/20% and over- and under-triage rates of 84% and 7%, respectively. After they merged, global sensitivity/specificity reached 87%/67%, and over- and under-triage rates were 71% and 3%, respectively CONCLUSIONS: A part the potential cost advantage achieved by the merger of two DCs, it can improve the quality of services to the population, reducing over- and under-triage and the use of lights and sirens and therefore, the risk of accidents. This is especially the case when a DC with poor triage performance merges with a high-performing DC.

Flebotomíneos de áreas com notificações de casos autóctones de leishmaniose visceral canina e leishmaniose tegumentar americana em Angra dos Reis, Rio de Janeiro, Brasil Phlebotomine fauna in areas of autochthonous cases of canine visceral leishmaniasis and American cutaneous leishmaniasis in Angra dos Reis, Rio de Janeiro, Brazil

Directory of Open Access Journals (Sweden)

Marcos Barbosa de Souza

2009-01-01

Full Text Available Flebótomos de áreas com notificações de casos autóctones de leishmaniose visceral canina e leishmaniose tegumentar americana em Angra dos Reis, Rio de Janeiro, Brasil. O município de Angra dos Reis apresenta casos humanos de leishmaniose tegumentar americana desde 1945. Inquéritos flebotomínicos realizados em 1978 revelaram a presença de Nyssomyia intermedia e a primeira notificação de Lutzomyia longipalpis no Rio de Janeiro, Ilha Grande, Angra dos Reis. Em agosto de 2002 foi notificado o primeiro caso canino de leishmaniose visceral na Ilha Grande, Angra dos Reis. Inquéritos flebotomínicos realizados nos peridomicílios, no período de novembro de 2002 a maio de 2003, em quatro localidades de Angra dos Reis, resultaram em 12.554 flebotomíneos e a presença de nove espécies: Brumptomyia sp.; Nyssomyia intermedia, Migonemyia migonei, Micropygomyia schreiberi, Pintomyia fischeri, Psychodopygus davisi, Psychodopygus ayrosai, Evandromyia tupinambay, Psathyromyia pelloni. foi Nyssomyia intermedia, predominante em todas as localidades, seguida por M.migonei. O principal vetor da LVA, Lutzomyia longipalpis, não foi detectado nas localidades, incluindo áreas do entorno, onde um cão infectado residia.The city of Angra dos Reis presents human cases of American cutaneous leishmaniasis since 1945. Phlebotomine surveys in 1978 had disclosed the presence of Nyssomyia intermedia and the first notification of Lutzomyia longipalpis in Rio de Janeiro, Ilha Grande, Angra dos Reis. In August of 2002 the first canine case of visceral leishmaniasis in the Ilha Grande, Angra dos Reis was notified. Surveys of phlebotomines in the peridomiciliary areas, in the period of November, 2002, through May, 2003, in four localities of Angra dos Reis, resulted in 12,554 specimens belonging to nine species: Brumptomyia sp. Nyssomyia intermedia, Migonemyia migonei, Micropygomyia schreiberi, Pintomyia fischeri, Psychodopygus davisi, Psychodopygus ayrosai

Leishmaniose tegumentar americana na Ilha Grande, Rio de Janeiro: VI. observações sobre a freqüência horária e variação mensal dos transmissores

Directory of Open Access Journals (Sweden)

Nelson A. de Araújo Filho

1981-12-01

Full Text Available Para estudo da freqüência horária dos principais vetores da Leishmaniose Tegumentar Americana (LTA, na Ilha Grande, durante os anos de 1976 a 1977, foram realizadas 4 capturas de 24 horas com isca animal (cão, ao ar livre, e 5 capturas de 24 horas em um único domicilio da área. Observou-se que a espécie Lutzomyia intermedia ocorreu durante todo o período noturno, com densidade constante no domicílio humano, enquanto a espécie Lutzomyia migonei apresentou maiores densidades no período da madrugada, nas capturas com isca animal (cão. Em 156 horas de estudo sobre variação mensal, durante o período de março de 1976 a fevereiro de 1977, a espécie L. intermedia ocorreu durante todo o ano, sendo abril, maio, outubro e dezembro os meses de maior densidade; a espécie L. migonei apresentou-se com baixa densidade, chegando a desaparecer no mês de setembro. As elevadas densidades das espécies L. intermedia e L. migonei indicam-nas como as prováveis vetoras de LTA na Ilha Grande.For the study of time frequency of the principal vectors of the Mucocutaneous Leishmaniasis in the Ilha Grande (Great Island, from 1976 to 1977 we made four catches in 24 hours with animal bait (dog in the outdoors and five catches in just one house of the area. It was found that the species Lutzomyia intermedia occurred during the whole night a steady density in human dwellings, while the species Lutzomyia migonei presented greater density at dawn, in catches with animal bait (dog. in a 156 hours' study on monthly variation, from March 1976 to February 1977, a specie Lutzomyia intermedia appeared during the whole year, the months of April, May, October and December being those of greater density while the specie Lutzomyia migonei had a low density even disappearing in September. The high densities of the species Lutzomyia intermedia and Lutzomyia migonei indica te them as the probable vectors of the mucocutaneous leishmaniasis in Ilha Grande.

De la urbanización a la intervención urbana estratégica: Instrumentos de planificación intermedia en Costa Rica

Directory of Open Access Journals (Sweden)

Roy Allan Jiménez Céspedes

2016-07-01

Full Text Available El presente artículo analiza el contexto y las posibilidades normativas del país para implementar estrategias de planificación de grandes conjuntos urbanos. Este tipo de planificación se ubica entre los planes reguladores locales y la ejecución del proyecto individual. Su aplicación tiene el alcance del conocido pero nunca aplicado “plan maestro”. Los actuales formatos de transformación del suelo en Costa Rica impiden un abordaje integral de grandes conjuntos urbanos. La separación práctica de la figura de la urbanización y del condominio ha generado una mejor salida en el mercado inmobiliario del segundo. Grandes áreas de la ciudad han sido intervenidas bajo el formato de condominio horizontal. Esta práctica aceptada e institucionalizada, provoca que la trama urbana existente experimente un proceso de sobrecarga. Al mismo tiempo, el condominio horizontal se considera exento de las cesiones que el desarrollo inmobiliario debe brindar a la ciudad. La renovación urbana, inexplorada pero existente en nuestra normativa, abraza muchas de las posibilidades para la implementación de diferentes procesos de escala intermedia. This paper analyzes the opportunities of Costa Rica to implement planning strategies for large urban sectors. This kind of planning takes place between municipal planning (local government and specific (urban projects, in which case, replaces the quite known but difficult to apply “master plan”. The current patterns of urban development and real estate in Costa Rica, prevent an integrated approach on large urban sectors. The lack of commercial advantages of the traditional urban development versus the gated community has provoked that large sectors of the city have been intervened as closed environments. The consequences of this very profitable and institutionalized practice are the overload on existing urban areas and the deficit of public facilities, which are necessary for an adequate urban growth. The urban

Leishmaniose tegumentar americana na Ilha Grande. Rio de Janeiro: V. Observações sobre a biologia dos transmissores em condiçoes naturais

Directory of Open Access Journals (Sweden)

Nelson A. de Araújo Filho

1981-12-01

Full Text Available Durante um surto de Leishmaniose Tegumentar Americana (LTA na Praia Vermelha, Ilha Grande, município de Angra dos Reis, Rio de Janeiro, foram coletados 4.192 exemplares de flebotomíneos em ambiente domiciliar e silvestre, com isca animal e outros abrigos naturais, durante o período de janeiro de 1976 a abril de 1977. Entre o total de 11 espécies coletadas encontraram-se 2.493 exemplares de Lutzomyia intermedia, 1.594 de Lutzomyia migonei e 105 exemplares de outras espécies. A L. intermedia apresentou densidade mais elevada no domicílio humano, enquanto a L. migonei apresentou coletas mais rendosas com iscas animais e galinheiros. O sinantropismo e a elevada densidade no ambiente domiciliar incriminam as espécies L. intermedia e L. migonei como as prováveis vetoras da LTA na Ilha Grande.

Inhibitory Effects of Lactoferrin on Growth and Biofilm Formation of Porphyromonas gingivalis and Prevotella intermedia▿

Science.gov (United States)

Wakabayashi, Hiroyuki; Yamauchi, Koji; Kobayashi, Tetsuo; Yaeshima, Tomoko; Iwatsuki, Keiji; Yoshie, Hiromasa

2009-01-01

Lactoferrin (LF) is an iron-binding antimicrobial protein present in saliva and gingival crevicular fluids, and it is possibly associated with host defense against oral pathogens, including periodontopathic bacteria. In the present study, we evaluated the in vitro effects of LF-related agents on the growth and biofilm formation of two periodontopathic bacteria, Porphyromonas gingivalis and Prevotella intermedia, which reside as biofilms in the subgingival plaque. The planktonic growth of P. gingivalis and P. intermedia was suppressed for up to 5 h by incubation with ≥130 μg/ml of human LF (hLF), iron-free and iron-saturated bovine LF (apo-bLF and holo-bLF, respectively), and ≥6 μg/ml of bLF-derived antimicrobial peptide lactoferricin B (LFcin B); but those effects were weak after 8 h. The biofilm formation of P. gingivalis and P. intermedia over 24 h was effectively inhibited by lower concentrations (≥8 μg/ml) of various iron-bound forms (the apo, native, and holo forms) of bLF and hLF but not LFcin B. A preformed biofilm of P. gingivalis and P. intermedia was also reduced by incubation with various iron-bound bLFs, hLF, and LFcin B for 5 h. In an examination of the effectiveness of native bLF when it was used in combination with four antibiotics, it was found that treatment with ciprofloxacin, clarithromycin, and minocycline in combination with native bLF for 24 h reduced the amount of a preformed biofilm of P. gingivalis compared with the level of reduction achieved with each agent alone. These results demonstrate the antibiofilm activity of LF with lower iron dependency against P. gingivalis and P. intermedia and the potential usefulness of LF for the prevention and treatment of periodontal diseases and as adjunct therapy for periodontal diseases. PMID:19451301

AVALIAÇÃO DA EFICIÊNCIA BANCÁRIA POR MEIO DA ABORDAGEM DE INTERMEDIAÇÃO: UMA ANÁLISE COMPARATIVA DE INSTITUIÇÕES FINANCEIRAS BRASILEIRAS

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Murilo Carneiro

Full Text Available Resumo Este estudo avalia, de forma comparativa, por meio da abordagem de intermediação, o grau de eficiência de 99 instituições financeiras que atuaram, em 2013, no mercado financeiro brasileiro. Foi a atividade de intermediação financeira que gerou o surgimento e a consolidação das instituições financeiras por todo o mundo. Portanto, essa foi uma das razões que motivou a escolha da abordagem de intermediação para se avaliar a eficiência das instituições financeiras. Acredita-se que seja relevante avaliar se tais instituições, no Brasil, estão conseguindo ser eficientes em sua atividade mais tradicional, a intermediação financeira. É importante ressaltar que, além da abordagem de intermediação, há também duas outras principais abordagens para se avaliar a eficiência das instituições financeiras: produção e rentabilidade. A técnica data envelopment analysis (DEA, cuja aplicabilidade para avaliação da eficiência bancária é muito difundida na literatura, foi adotada para calcular os escores de eficiência das instituições, que foram classificadas de acordo com quatro critérios: origem do capital, porte, risco (rating de crédito e setor de atuação. Foram identificadas 22 instituições com maior grau de eficiência, havendo certa heterogeneidade em relação à origem do capital, porte e setor de atuação. Por outro lado, foi identificado um indício de que há uma relação diretamente proporcional positiva entre o rating de crédito e a eficiência.

Thalassemia intermedia: An overview

African Journals Online (AJOL)

Rabah M. Shawky

2012-04-25

Apr 25, 2012 ... abnormalities occur due to massive expansion of erythroid bone marrow activity ..... [3] Savage D, Wood NAP, Bidwell JL, Fitches A, Old JM, Hui KM. Detection of b-thalassemia mutations using DNA heteroduplex generator ...

Preparation of 11C-labelled methanol on alumina column

International Nuclear Information System (INIS)

Sarkadi, E.; Kovacs, Z.; Horvath, G.

1998-01-01

The [ 11 C]methyl iodide is an important intermedia to synthesize 11 C-labelled radiopharmaceuticals for medical diagnostics in positron emission tomography. Recently a new method has been developed to produce [ 11 C]methanol intermedia. The advantage of this method of radiomethanol preparation is the application of an alumina column at room temperature instead of a complicated cooling unit used with the conventional reaction vessel. The yield and purity of radiomethanol was the same as in the previous methods. (K.A.)

The phlebotomine sand flies fauna in Parque Estadual do Rio Doce, Minas Gerais, Brazil.

Science.gov (United States)

de Souza, Cristian Ferreira; Brazil, Reginaldo Peçanha; Bevilacqua, Paula Dias; Andrade Filho, Jose Dilermando

2015-12-02

Phlebotomine sand flies are dipterans of the family Psychodidae. They are very important to veterinary medicine because some species are vectors of infective forms of Leishmania spp., the etiological agents of leishmaniasis. The Parque Estadual do Rio Doce is located in an area with constant reports of cases of leishmaniasis. In order to better understanding the phlebotamine sand fly fauna of the park, the present work was undertaken with the goal of analyzing phlebotomine sand flies collected there, verifying their seasonality and correlating their presence with forest and/or anthropic areas. To analyze the fauna of phlebotomine sand flies, HP-type, model CDC light traps were distributed along the Juquita trail of PERD. Twelve traps were installed between September 2012 and February 2014, and captured specimens were identified to species. A total of 1993 phlebotomine sand flies of 30 species were captured. The most abundant species were Pressatia choti, Psychodopygus davisi and Nyssomyia intermedia. The high number of Nyssomyia intermedia captured drew attention because they are considered one of the vectors of the infective Leishmania braziliensis present at PERD. No seasonality was observed in the occurrence of phlebotomine sand flies captured at PERD. The number of captured specimens of vector species, and the distance of traps from the forest boarder, were negatively correlated, showing that these vectors (Nyssomyia intermedia, Nyssomyia whitmani and Migonemyia migonei) were less common inside the forest area and that attention should be drawn to other potential vector species in the forest. These results can contribute to leishmaniasis prevention strategies directed at the visitors and professionals at or near PERD. The finding of the presence of Leishmania vectors in the park area must be given attention, since disease transmission can threaten people who visit PERD and its surroundings. Therefore, information on the prevention of leishmaniasis needs to be

Warnings to the far future

International Nuclear Information System (INIS)

Posner, R.

1990-01-01

Inquiries were launched in the USA, in Poland, France, Hungary, Brazil, and the Federal Republic of Gemany to find semiotic solutions to problems arising from the communication of radioactive waste locations and dangers to posteriority. How can information be communicated over a period of 10000 years? What has been communicated to us from the past 10000 years? It seems to be sure that neither natural nor artificial barriers nor remnants (e.g. skeletons) or sources (today's communication), but only explicit messages will keep intelligent creatures from intrusion into radioactive waste depositories. Among the subjects dealt with are: Pandora's box and how to prevent its opening; mathematical coding on living sign matter; living detectors and complementary signs: ray coat, broken eye, and nuclear sirens; the three-chamber system: a way to the democratic organization of collective knowledge and conscience which can survive millennia. (orig./HSCH) [de

Warnings to the far future. Radioactive wastes as a communications problem. Warnungen an die ferne Zukunft. Atommuell als Kommunikationsproblem

Energy Technology Data Exchange (ETDEWEB)

Posner, R. (Technische Univ. Berlin (Germany, F.R.). Arbeitsstelle fuer Semiotik) (ed.)

1990-01-01

Inquiries were launched in the USA, in Poland, France, Hungary, Brazil, and the Federal Republic of Gemany to find semiotic solutions to problems arising from the communication of radioactive waste locations and dangers to posteriority. How can information be communicated over a period of 10000 years What has been communicated to us from the past 10000 years It seems to be sure that neither natural nor artificial barriers nor remnants (e.g. skeletons) or sources (today's communication), but only explicit messages will keep intelligent creatures from intrusion into radioactive waste depositories. Among the subjects dealt with are: Pandora's box and how to prevent its opening; mathematical coding on living sign matter; living detectors and complementary signs: ray coat, broken eye, and nuclear sirens; the three-chamber system: a way to the democratic organization of collective knowledge and conscience which can survive millennia. (orig./HSCH).

Sirenomelia in Bmp7 and Tsg compound mutant mice: requirement for Bmp signaling in the development of ventral posterior mesoderm.

Science.gov (United States)

Zakin, Lise; Reversade, Bruno; Kuroda, Hiroki; Lyons, Karen M; De Robertis, Eddy M

2005-05-01

Sirenomelia or mermaid-like phenotype is one of the principal human congenital malformations that can be traced back to the stage of gastrulation. Sirenomelia is characterized by the fusion of the two hindlimbs into a single one. In the mouse, sirens have been observed in crosses between specific strains and as the consequence of mutations that increase retinoic acid levels. We report that the loss of bone morphogenetic protein 7 (Bmp7) in combination with a half dose or complete loss of twisted gastrulation (Tsg) causes sirenomelia in the mouse. Tsg is a Bmp- and chordin-binding protein that has multiple effects on Bmp metabolism in the extracellular space; Bmp7 is one of many Bmps and is shown here to bind to Tsg. In Xenopus, co-injection of Tsg and Bmp7 morpholino oligonucleotides (MO) has a synergistic effect, greatly inhibiting formation of ventral mesoderm and ventral fin tissue. In the mouse, molecular marker studies indicate that the sirenomelia phenotype is associated with a defect in the formation of ventroposterior mesoderm. These experiments demonstrate that dorsoventral patterning of the mouse posterior mesoderm is regulated by Bmp signaling, as is the case in other vertebrates. Sirens result from a fusion of the hindlimb buds caused by a defect in the formation of ventral mesoderm.

Protection of G2 and G3 against CO{sub 2}; La protection contre le CO{sub 2} des ensembles G.2 et G.3

Energy Technology Data Exchange (ETDEWEB)

Chassany, J Ph; Rodier, J [Commissariat a l' Energie Atomique, Service de Protection contre les Radiations, Marcoule (France). Centre d' Etudes Nucleaires

1961-07-01

The presence of 60.000 m{sup 3} of CO{sub 2} at 15 kg/cm{sup 2} pressure has made necessary to set up a detection and protection system on a scale equal to that used for ionising radiations. Instruments to check CO and CO{sub 2} in the atmosphere carry out measurements continuously, alarm systems give warning if the CO{sub 2} content increases, and the working areas may be surveyed by a whole series of portable instruments. The order for evacuation is given by sirens, and respiratory units are placed at strategic points along the exit paths. (author) [French] La presence de 60000 m{sup 3} de CO{sub 2} a 15 kg/cm{sup 2} de pression a exige la mise en place d'un dispositif de detection et de protection aussi important que celui realise pour les radiations ionisantes. Des appareils de controle d'ambiance pour le CO et le CO{sub 2} effectuent des mesures en permanence, des appareils d'alarme donnent l'alerte en cas d'augmentation de la teneur en CO{sub 2} et tout une serie d'appareils portatifs permettant la surveillance des chantiers. L'evacuation est demandee par sirene et des appareils respiratoires autonomes jalonnent les trajets vers les sorties. (auteur)

Moving Stimuli Facilitate Synchronization But Not Temporal Perception.

Science.gov (United States)

Silva, Susana; Castro, São Luís

2016-01-01

Recent studies have shown that a moving visual stimulus (e.g., a bouncing ball) facilitates synchronization compared to a static stimulus (e.g., a flashing light), and that it can even be as effective as an auditory beep. We asked a group of participants to perform different tasks with four stimulus types: beeps, siren-like sounds, visual flashes (static) and bouncing balls. First, participants performed synchronization with isochronous sequences (stimulus-guided synchronization), followed by a continuation phase in which the stimulus was internally generated (imagery-guided synchronization). Then they performed a perception task, in which they judged whether the final part of a temporal sequence was compatible with the previous beat structure (stimulus-guided perception). Similar to synchronization, an imagery-guided variant was added, in which sequences contained a gap in between (imagery-guided perception). Balls outperformed flashes and matched beeps (powerful ball effect) in stimulus-guided synchronization but not in perception (stimulus- or imagery-guided). In imagery-guided synchronization, performance accuracy decreased for beeps and balls, but not for flashes and sirens. Our findings suggest that the advantages of moving visual stimuli over static ones are grounded in action rather than perception, and they support the hypothesis that the sensorimotor coupling mechanisms for auditory (beeps) and moving visual stimuli (bouncing balls) overlap.

First assessment of low- to medium-temperature geothermal reserves in 20 Mexican states; Primera estimacion de las reservas geotermicas de temperatura intermedia a baja en veinte estados de Mexico

Energy Technology Data Exchange (ETDEWEB)

Iglesias, Eduardo R.; Torres, Rodolfo J. [Instituto de Investigaciones Electricas, Gerencia de Geotermia, Cuernavaca, Morelos (Mexico)]. E-mail: iglesias@iie.org.mx

2009-07-15

A first, partial, assessment is included of the low- to medium-temperature geothermal reserves in 20 Mexican States and their aggregate value. The assessment covers about 29.16% of the identified geothermal-surface manifestations in the public database. For reserve assessments, we use the volumetric method, supplemented with Montecarlo simulations and statistics, to quantify inherent uncertainties. Our estimations are presented on a state-by-state basis. We estimate the aggregated reserves of the 20 states as between 7.7 x 1016 and 8.6 x 1016 kJ, with 90% confidence. The most likely reservoir temperatures range between 60-180 degrees Celsius, with a mean of 111 degrees Celsius. Such massive amounts of recoverable energy-and the associated temperatures-are potentially important for the economic development of nearby localities and the nation. [Spanish] En este trabajo se hace una primera estimacion, parcial, de las reservas geotermicas de temperatura intermedia a baja de Mexico. La estimacion incluye 29.16% de las manifestaciones geotermicas identificadas en la base de datos publica utilizada. Para estimar las reservas se utilizo el metodo de volumen, suplementado con simulaciones por el metodo de Montecarlo, con el fin de cuantificar las incertidumbres inherentes. Las estimaciones se presentan estado por estado. Estos resultados indican que las reservas agregadas de los 20 estados considerados estan entre 7.7 x 1016 y 8.6 x 1016 kJ, con 90% de confianza. La distribucion de las temperaturas de yacimiento mas probables varia entre aproximadamente 60 y 180 grados centigrados, con un valor medio de 111 grados centigrados. La enorme magnitud de estas reservas, y sus temperaturas asociadas, son potencialmente importantes para el desarrollo economico de las poblaciones ubicadas en su cercania.

Comparison of Riboflavin and Toluidine Blue O as Photosensitizers for Photoactivated Disinfection on Endodontic and Periodontal Pathogens In Vitro

DEFF Research Database (Denmark)

Nielsen, Henrik Krarup; Garcia, Javier; Væth, Michael

2015-01-01

, Lactobacillus paracasei, Porphyromonas gingivalis, Prevotella intermedia and Propionibacterium acnes) were subjected to photoactivated disinfection with riboflavin/blue light and toluidine blue O/red light, and survival rates were determined by CFU counts. Within the limited irradiation time of one minute......, photoactivated disinfection with riboflavin/blue light only resulted in minor reductions in CFU counts, whereas full kills were achieved for all organisms when using toluidine blue O/red light. The black pigmented anaerobes P. gingivalis and P. intermedia were eradicated completely by riboflavin/blue light...

Responses to playback of different subspecies songs in the Reed Bunting (Emberiza s. schoeniclus)

DEFF Research Database (Denmark)

Matessi, Giuliano; Dabelsteen, Torben; Pilastro, A.

2000-01-01

Populations of Reed Buntings Emberiza schoeniclus in the western Palearctic are classified in two major subspecies groups according to morphology: northern migratory schoeniclus and Mediterranean resident intermedia. Songs of the two groups differ mainly in complexity and syllable structure......, with intermedia songs being more complex. We explored the possibilities of song as a subspecies isolating mechanism by testing if male schoeniclus Reed Buntings reacted differently to field playbacks of songs from their own subspecies group, from the foreign subspecies group and from a control species...

ORAL PERFORMANCE AS SIREN The Example of Ozoemena Nwa ...

African Journals Online (AJOL)

This brand of music was peddled and is still being peddled by some ... propagation of new ideas and or condemning of deviant behavior. Fully aware of the .... one can describe Ozoemena's commentaries on the position of the. Igbo race in the ...

Molecular and Clinical Based Cardiovascular Care Program

Science.gov (United States)

2007-01-01

civilian collaboration between WRMC, Windber Medical Center (WMC) /Windber Research Institute (WRI) is to: 1) Teach , implement and study lifestyle...Walter Reed Army Medical Center (WRAMC) and Windber Medical Center (WMC)/Windber Research Institute (WRI) is to: 1) Teach , implement and study...995. p. 114 • 11 ~. 15. Ornit.h D, Sd1erwilz LW, Doody ~S, et al. Efleds of siren mone1gemenl training and dietary changes in trealin9 isehornie

Tsunamis: Detection, monitoring, and early-warning technologies

Digital Repository Service at National Institute of Oceanography (India)

Joseph, A.

upon, which are relevant to tsunami warning, include public announcement siren systems and numerical models. Special attention has been paid to the submarine cable-mounted systems. Due importance has been attached to the instrumented era which... of appropriate emergency measures. Whereas satellite-based reporting is one option, a simple and cost-effective methodology for real-time reporting of tsunami from coastal regions and islands is cellular based GPRS technology. From an operational point...

Acoustic Excitation of Liquid Fuel Droplets and Coaxial Jets

Science.gov (United States)

2009-01-01

would also like to acknowledge the support of the NASA Microgravity Combustion program which made possible the completion of this research and Maj...fuels exposed to different acoustic excitation conditions in a laboratory environment and during free-fall (microgravity) conditions in a NASA drop tower...then sent to two amplifiers, one for each piezo-siren. The amplifiers were a Krohn-Hite (model 7500) and a Trek (model PZD2000A), which amplified the

Droplet Combustion and Non-Reactive Shear-Coaxial Jets with Transverse Acoustic Excitation

Science.gov (United States)

2012-06-01

for this. Recent studies at UCLA and at NASA Glenn Research Center by Dattarajan et al. [20, 21] have focused on methanol droplet combustion...via Trek PZD2000A high-voltage amplifiers, to each piezo-siren. The waveform generators output signals were locked in frequency. However, their phase...1.3. Verify the wire on Channel 1 of the Tenma oscilloscope (Model No. 72-6800) comes from the output voltage monitor on the Trek -1 amplifier

Auditory Perception in an Open Space: Detection and Recognition

Science.gov (United States)

2015-06-01

recognition ranges of most sounds were approximately 100–200 m. Therefore, it may be hypothesized that this range makes up the soundscape or the range of the... soundscapes . Acta Acustica united with Acustica. 2003;89:287–295. Delaney ME. Range predictions for siren sources. Teddington (UK): National...management of park soundscapes : a review. Applied Acoustics. 2008;69:77–92. Mirabella A, Goldstein D. The effects of ambient noise upon signal detection

Férula quirúrgica intermedia en cirugía ortognática bimaxilar: Un método simple de obtención Intermediate surgical splint in orthognathic bimaxillary surgery: A simple method for obtaining it

Directory of Open Access Journals (Sweden)

J.V. Pascual Gil

2004-12-01

Full Text Available La utilización de la férula quirúrgica intermedia (FQI en las intervenciones de cirugía ortognática bimaxilar es un procedimiento habitual. La utilidad, ayuda y confianza que aporta es indiscutible. Sin embargo, el procedimiento clásico de obtención de la FQI es complejo. Proponemos simplificar la fabricación de la FQI sin renunciar a su precisión y fiabilidad. Debemos cuestionar algunos conceptos del método de obtención de esta férula y reflexionar sobre el siguiente concepto básico: la dimensión vertical craneomandibular preoperatoria es igual a la dimensión vertical craneomandibular postoperatoria con la férula en posición. Para confeccionarla se ha diseñado un nuevo dispositivo denominado: Posicionador del maxilar.The employment of the intermediate surgical splint in bimaxillary orthognatic surgery is a common procedure. The utility, aid and confidence which are provided are inquestionable. Anyway the classic procedure of attainment of the splint is complex. We propose to simplify the manufacturing of the splint without renouncing to it´s accuracy and reliability. We must question some concepts about the attainment method of this splint and consider the following basic concept: the vertical preoperatory craneomandibular dimension is the same as the vertical postoperatory craneomandibular dimension with the splint in position. To manufacture it we have designed a new device called: Maxillary Placet.

Recombinant Protein Containing B-Cell Epitopes of Different Loxosceles Spider Toxins Generates Neutralizing Antibodies in Immunized Rabbits.

Science.gov (United States)

Lima, Sabrina de Almeida; Guerra-Duarte, Clara; Costal-Oliveira, Fernanda; Mendes, Thais Melo; Figueiredo, Luís F M; Oliveira, Daysiane; Machado de Avila, Ricardo A; Ferrer, Valéria Pereira; Trevisan-Silva, Dilza; Veiga, Silvio S; Minozzo, João C; Kalapothakis, Evanguedes; Chávez-Olórtegui, Carlos

2018-01-01

Loxoscelism is the most important form of araneism in South America. The treatment of these accidents uses heterologous antivenoms obtained from immunization of production animals with crude loxoscelic venom. Due to the scarcity of this immunogen, new alternatives for its substitution in antivenom production are of medical interest. In the present work, three linear epitopes for Loxosceles astacin-like protease 1 (LALP-1) (SLGRGCTDFGTILHE, ENNTRTIGPFDYDSIMLYGAY, and KLYKCPPVNPYPGGIRPYVNV) and two for hyaluronidase (LiHYAL) (NGGIPQLGDLKAHLEKSAVDI and ILDKSATGLRIIDWEAWR) from Loxosceles intermedia spider venom were identified by SPOT-synthesis technique. One formerly characterized linear epitope (DFSGPYLPSLPTLDA) of sphingomyelinase D (SMase D) SMase-I from Loxosceles laeta was also chosen to constitute a new recombinant multiepitopic protein. These epitopes were combined with a previously produced chimeric multiepitopic protein (rCpLi) composed by linear and conformational B-cell epitopes from SMase D from L. intermedia venom, generating a new recombinant multiepitopic protein derived from loxoscelic toxins (rMEPLox). We demonstrated that rMEPLox is non-toxic and antibodies elicited in rabbits against this antigen present reactivity in ELISA and immunoblot assays with Brazilian L. intermedia, L. laeta, L. gaucho , and L. similis spider venoms. In vivo and in vitro neutralization assays showed that anti-rMEPLox antibodies can efficiently neutralize the sphingomyelinase, hyaluronidase, and metalloproteinase activity of L. intermedia venom. This study suggests that this multiepitopic protein can be a suitable candidate for experimental vaccination approaches or for antivenom production against Loxosceles spp. venoms.

Recombinant Protein Containing B-Cell Epitopes of Different Loxosceles Spider Toxins Generates Neutralizing Antibodies in Immunized Rabbits

Science.gov (United States)

Lima, Sabrina de Almeida; Guerra-Duarte, Clara; Costal-Oliveira, Fernanda; Mendes, Thais Melo; Figueiredo, Luís F. M.; Oliveira, Daysiane; Machado de Avila, Ricardo A.; Ferrer, Valéria Pereira; Trevisan-Silva, Dilza; Veiga, Silvio S.; Minozzo, João C.; Kalapothakis, Evanguedes; Chávez-Olórtegui, Carlos

2018-01-01

Loxoscelism is the most important form of araneism in South America. The treatment of these accidents uses heterologous antivenoms obtained from immunization of production animals with crude loxoscelic venom. Due to the scarcity of this immunogen, new alternatives for its substitution in antivenom production are of medical interest. In the present work, three linear epitopes for Loxosceles astacin-like protease 1 (LALP-1) (SLGRGCTDFGTILHE, ENNTRTIGPFDYDSIMLYGAY, and KLYKCPPVNPYPGGIRPYVNV) and two for hyaluronidase (LiHYAL) (NGGIPQLGDLKAHLEKSAVDI and ILDKSATGLRIIDWEAWR) from Loxosceles intermedia spider venom were identified by SPOT-synthesis technique. One formerly characterized linear epitope (DFSGPYLPSLPTLDA) of sphingomyelinase D (SMase D) SMase-I from Loxosceles laeta was also chosen to constitute a new recombinant multiepitopic protein. These epitopes were combined with a previously produced chimeric multiepitopic protein (rCpLi) composed by linear and conformational B-cell epitopes from SMase D from L. intermedia venom, generating a new recombinant multiepitopic protein derived from loxoscelic toxins (rMEPLox). We demonstrated that rMEPLox is non-toxic and antibodies elicited in rabbits against this antigen present reactivity in ELISA and immunoblot assays with Brazilian L. intermedia, L. laeta, L. gaucho, and L. similis spider venoms. In vivo and in vitro neutralization assays showed that anti-rMEPLox antibodies can efficiently neutralize the sphingomyelinase, hyaluronidase, and metalloproteinase activity of L. intermedia venom. This study suggests that this multiepitopic protein can be a suitable candidate for experimental vaccination approaches or for antivenom production against Loxosceles spp. venoms. PMID:29666624

Prevalence of intestinal pathogens in Danish finishing pig herds

DEFF Research Database (Denmark)

Stege, H.; Jensen, Tim Kåre; Møller, Kristian

2000-01-01

Our aim was to determine the prevalence of the intestinal bacteria: Lawsonia intracellularis, Brachyspira hyodysenteriae, Serpulina intermedia, Brachyspira innocens, Brachyspira pilosicoli, pathogenic Escherichia coli (serogroups 0138, 0139, 0141 and 0149) and Salmonella enterica in Danish...... were collected and examined by polymerase chain reaction (PCR) or culture. L. intracellularis was found in 74 herds (93.7%), B. hyodysenteriae in two herds (2.5%), S. intermedia in 10 herds (12.7%), B. innocens in 27 herds (34.2%), B. pilosicoli in 15 herds (19.0%), pathogenic E. call in 19 herds (24...

Using On-scene EMS Responders' Assessment and Electronic Patient Care Records to Evaluate the Suitability of EMD-triaged, Low-acuity Calls for Secondary Nurse Triage in 911 Centers.

Science.gov (United States)

Scott, Greg; Clawson, Jeff; Fivaz, Mark C; McQueen, Jennie; Gardett, Marie I; Schultz, Bryon; Youngquist, Scott; Olola, Christopher H O

2016-02-01

Using the Medical Priority Dispatch System (MPDS) - a systematic 911 triage process - to identify a large subset of low-acuity patients for secondary nurse triage in the 911 center is a largely unstudied practice in North America. This study examines the ALPHA-level subset of low-acuity patients in the MPDS to determine the suitability of these patients for secondary triage by evaluating vital signs and necessity of lights-and-siren transport, as determined by attending Emergency Medical Services (EMS) ambulance crews. The primary objective of this study was to determine the clinical status of MPDS ALPHA-level (low-acuity) patients, as determined by on-scene EMS crews' patient care records, in two US agencies. A secondary objective was to determine which ALPHA-level codes are suitable candidates for secondary triage by a trained Emergency Communication Nurse (ECN). In this retrospective study, one full year (2013) of both dispatch data and EMS patient records data, associated with all calls coded at the ALPHA-level (low-acuity) in the dispatch protocol, were collected. The primary outcome measure was the number and percentage of ALPHA-level codes categorized as low-acuity, moderate-acuity, high-acuity, and critical using four common vital signs to assign these categories: systolic blood pressure (SBP), pulse rate (PR), oxygen saturation (SpO2), and Glasgow Coma Score (GCS). Vital sign data were obtained from ambulance crew electronic patient care records (ePCRs). The secondary endpoint was the number and percentage of ALPHA-level codes that received a "hot" (lights-and-siren) transport. Out of 19,300 cases, 16,763 (86.9%) were included in the final analysis, after excluding cases from health care providers and those with missing data. Of those, 89% of all cases did not have even one vital sign indicator of unstable patient status (high or critical vital sign). Of all cases, only 1.1% were transported lights-and-siren. With the exception of the low-acuity, ALPHA

Droplet Combustion and Non-Reactive Shear-Coaxial Jets with and without Transverse Acoustic Excitation

Science.gov (United States)

2012-01-01

node, there is no droplet deflection, but there is limited evidence for this. Recent studies at UCLA and at NASA Glenn Research Center by Dattarajan et...generator supplied continuous sine wave signals, which were amplified via Trek PZD2000A high-voltage amplifiers, to each piezo-siren. The waveform...1.3. Verify the wire on Channel 1 of the Tenma oscilloscope (Model No. 72-6800) comes from the output voltage monitor on the Trek -1 amplifier and the

Numerical modelling and evacuation strategies for tsunami awareness: lessons from the 2012 Haida Gwaii Tsunami

OpenAIRE

Santos, Angela; Tavares, Alexandre Oliveira; Queirós, Margarida

2016-01-01

On October 28, 2012, an earthquake occurred offshore Canada, with a magnitude Mw of 7.8, triggering a tsunami that propagated through the Pacific Ocean. The tsunami numerical model results show it would not be expected to generate widespread inundation on Hawaii. Yet, two hours after the earthquake, the Pacific Tsunami Warning Centre (PTWC) issued a tsunami warning to the state of Hawaii. Since the state was hit by several tsunamis in the past, regular siren exercises, tsuna...

Mixing in Shear Coaxial Jets (Briefing Charts)

Science.gov (United States)

2013-08-01

relevant boundary layers 9. Thermodynamic states (2 phase, 1 phase) 10. Transverse Acoustic mode from chamber/siren, f=f(c, geometry St=fDij/Uij 11...stability theory for inviscid instability of a hyperbolic tangent velocity profile for free boundary layers • U(y)=0.5[1 + tanh(y)] • Chigier and Beer , 1964...acoustics Natural OJ excited IJ excited From Chigier NA. and Beer JM, The Flow Region Near the Nozzle in Double Concentric Jets, J of

Synergy in biofilm formation between Fusobacterium nucleatum and Prevotella species.

Science.gov (United States)

Okuda, Tamaki; Kokubu, Eitoyo; Kawana, Tomoko; Saito, Atsushi; Okuda, Katsuji; Ishihara, Kazuyuki

2012-02-01

The formation of biofilm by anaerobic, Gram-negative bacteria in the subgingival crevice plays an important role in the development of chronic periodontitis. The aim of this study was to characterize the role of coaggregation between Fusobacterium nucleatum and Prevotella species in biofilm formation. Coaggregation between F. nucleatum and Prevotella species was determined by visual assay. Effect of co-culture of the species on biofilm formation was assessed by crystal violet staining. Effect of soluble factor on biofilm formation was also examined using culture supernatant and two-compartment co-culture separated by a porous membrane. Production of autoinducer-2 (AI-2) by the organisms was evaluated using Vibrio harveyi BB170. Cells of all F. nucleatum strains coaggregated with Prevotella intermedia or Prevotella nigrescens with a score of 1-4. Addition of ethylenediamine tetraacetic acid or l-lysine inhibited coaggregation. Coaggregation disappeared after heating of P. intermedia or P. nigrescens cells, or Proteinase K treatment of P. nigrescens cells. Co-culture of F. nucleatum ATCC 25586 with P. intermedia or P. nigrescens strains increased biofilm formation compared with single culture (p culture with culture supernatant of these strains, however, did not enhance biofilm formation by F. nucleatum. Production of AI-2 in Prevotella species was not related to enhancement of biofilm formation by F. nucleatum. These findings indicate that physical contact by coaggregation of F. nucleatum strains with P. intermedia or P. nigrescens plays a key role in the formation of biofilm by these strains. Copyright © 2011 Elsevier Ltd. All rights reserved.

The Phlebotominae sand fly (Diptera: Psychodidae fauna of two Atlantic Rain Forest Reserves in the State of Rio de Janeiro, Brazil

Directory of Open Access Journals (Sweden)

Souza Nataly A

2001-01-01

Full Text Available During two consecutive years, studies on the sand fly fauna in Poço das Antas and Fazenda Bom Retiro, two Atlantic Rain Forest Reserves from the State of Rio de Janeiro, were performed using Shannon traps, CDC light traps and human bait collections. Eleven species were identified; Lutzomyia longipalpis, L. migonei, L. edwardsi, L. intermedia, L. whitmani, L. fischeri, L. shannoni, L. ayrozai, L. hirsuta, L. monticola and L. misionensis (first occurrence in the State of Rio de Janeiro. L. intermedia and L. whitmani were the predominant anthropophilic species around houses, while L. hirsuta predominated in the forest.

Diet and dietary overlap of three sympatric fish species in lakes of the upper Paraná River floodplain

Directory of Open Access Journals (Sweden)

Karla Danielle Gaspar da Luz

1999-01-01

Full Text Available For a dietary study, specimens of Astyanax bimaculatus, Astyanax schubarti, and Moenkhausia intermedia were caught monthly with seines-nets in six lakes (Pau Veio, Porto Rico, Três Amigos, Mutum, Pontal, and Canal do Meio, on the floodplain of the Upper Paraná River, from March 1992 through February 1993. We analyzed stomach contents of 599 A. bimaculatus, 293 A. schubarti, and 394 M. intermedia, by the Occurrence and Points methods, combined in the Alimentary Index (AI. This analysis revealed that these species have broad dietary spectra. A. bimaculatus was an omnivore, with a tendency toward herbivory-insectivory. In most of the lakes it consumed terrestrial insects, followed by higher plants and microcrustaceans. A. schubarti was an omnivore, consuming the same type of items in the different lakes, in different proportions depending on the locality. M. intermedia was a planktophage, feeding mainly on cladocerans (more than 50% in all the lakes except for Pontal, where higher plants were predominant (45.1%. Although A. bimaculatus and A. schubarti consumed similar items in the different localities, detrended correspondence analysis (DCA showed distinct segregation among the three species in all lakes studied.Para estudo da dieta foram capturados mensalmente exemplares de Astyanax bimaculatus, Astyanax schubarti e Moenkhausia intermedia com redes de arraste, em seis lagoas (Pau Velho, Porto Rico, Três Amigos, Mutum, Pontal e Canal do Meio, na planície de inundação do alto rio Paraná, de março/92 a fevereiro/93. Foram analisados 599 conteúdos gástricos de A. bimaculatus, 293 de A. schubarti e 394 de M. intermedia, pelos métodos de Ocorrência e Pontos, combinados no Índice Alimentar (IAi. Tal análise revelou que estas espécies apresentam amplo espectro alimentar. A. bimaculatus foi caracterizada como onívora com tendência a herbivoria-insetivoria, consumindo, na maioria das lagoas, insetos terrestres, seguidos de vegetal superior

Intermedia: Tomorrow's AV Experience Today

Science.gov (United States)

McVey, G. F.

1970-01-01

The coordinator of the Multimedia Instructional Laboratory at Wisconsin describes an experimental course whose primary objective was to expose through direct student participation the relationships and processes involved when messages are presented through several media simultaneously." (Author/LS)

Abscisic acid regulates seed germination of Vellozia species in response to temperature.

Science.gov (United States)

Vieira, B C; Bicalho, E M; Munné-Bosch, S; Garcia, Q S

2017-03-01

The relationship between the phytohormones, gibberellin (GA) and abscisic acid (ABA) and light and temperature on seed germination is still not well understood. We aimed to investigate the role of the ABA and GA on seed germination of Vellozia caruncularis, V. intermedia and V. alutacea in response to light/dark conditions on different temperature. Seeds were incubated in GA (GA 3 or GA 4 ) or ABA and their respective biosynthesis inhibitors (paclobutrazol - PAC, and fluridone - FLU) solutions at two contrasting temperatures (25 and 40 °C). Furthermore, endogenous concentrations of active GAs and those of ABA were measured in seeds of V. intermedia and V. alutacea during imbibition/germination. Exogenous ABA inhibited the germination of Vellozia species under all conditions tested. GA, FLU and FLU + GA 3 stimulated germination in the dark at 25 °C (GA 4 being more effective than GA 3 ). PAC reduced seed germination in V. caruncularis and V. alutacea, but did not affect germination of V. intermedia at 40 °C either under light or dark conditions. During imbibition in the dark, levels of active GAs decreased in the seeds of V. intermedia, but were not altered in those of V. alutacea. Incubation at 40 °C decreased ABA levels during imbibition in both V. caruncularis and V. alutacea. We conclude that the seeds of Vellozia species studied here require light or high temperature to germinate and ABA has a major role in the regulation of Vellozia seed germination in response to light and temperature. © 2016 German Botanical Society and The Royal Botanical Society of the Netherlands.

Clinical and Microbial Evaluation of the Effects on Gingivitis of a Mouth Rinse Containing an Enteromorpha linza Extract

Science.gov (United States)

Cho, Han-Bin; Lee, Hee-Hyun; Lee, Ok-Hwan; Choi, Hyeon-Son; Choi, Jae-Suk

2011-01-01

Abstract Enteromorpha linza, a green alga, has been recognized as a potential source of natural antimicrobial and antifungal compounds. We previously reported that an E. linza extract strongly inhibited the growth of Prevotella intermedia and Porphyromonas gingivalis. The principal objective of this study was to evaluate the clinical effect of a mouth rinse containing the E. linza extract on gingivitis disease, as measured by the plaque index (PI), gingival index (GI), and bleeding on probing (BOP), and on two bacterial strains (P. intermedia and P. gingivalis), in comparison with Listerine® (Listerine-Korea, Seoul, Korea), which was used as a positive control. In total, 55 subjects were recruited into active participation in this clinical study. The PI, GI, BOP, and bacterial strains were then evaluated over a test period of 6 weeks. After 1, 2, 4, and 6 weeks, the same clinical indices were recorded, and the levels of P. intermedia and P. gingivalis were quantified via real-time polymerase chain reaction. At the end of the study, the group using the mouth rinse containing the E. linza extract evidenced significant reductions in the clinical indices (PI, GI, and BOP) and P. gingivalis compared with baseline values. Moreover, E. linza extract containing mouth rinse produced effects similar to those of Listerine. Overall, these results indicate that a mouth rinse containing E. linza extract significantly reduces plaque, improves the condition of gingival tissues, and reduces bleeding. Additionally, E. linza extract mouth rinse significantly inhibits P. gingivalis and P. intermedia. Thus, this clinical study demonstrated that the twice-daily use of an E. linza extract mouth rinse can inhibit and prevent gingivitis. PMID:22145775

The Siren song of vocal fundamental frequency for romantic relationships

Directory of Open Access Journals (Sweden)

Sarah eWeusthoff

2013-07-01

Full Text Available A multitude of factors contribute to why and how romantic relationships are formed as well as whether they ultimately succeed or fail. Drawing on evolutionary models of attraction and speech production as well as integrative models of relationship functioning, this review argues that paralinguistic cues (more specifically the fundamental frequency of the voice that are initially a strong source of attraction also increase couples’ risk for relationship failure. Conceptual similarities and differences between the multiple operationalizations and interpretations of vocal fundamental frequency are discussed and guidelines are presented for understanding both convergent and non-convergent findings. Implications for clinical practice and future research are discussed.

Peering beyond the horizon with standard sirens and redshift drift

Science.gov (United States)

Jimenez, Raul; Raccanelli, Alvise; Verde, Licia; Matarrese, Sabino

2018-04-01

An interesting test on the nature of the Universe is to measure the global spatial curvature of the metric in a model independent way, at a level of |Ωk|limit of |Ωk|<10‑4 would yield stringent tests on several models of inflation. Further, improving the constraint by an order of magnitude would help in reducing "model confusion" in standard parameter estimation. Moreover, if the curvature is measured to be at the value of the amplitude of the CMB fluctuations, it would offer a powerful test on the inflationary paradigm and would indicate that our Universe must be significantly larger than the current horizon. On the contrary, in the context of standard inflation, measuring a value above CMB fluctuations will lead us to conclude that the Universe is not much larger than the current observed horizon; this can also be interpreted as the presence of large fluctuations outside the horizon. However, it has proven difficult, so far, to find observables that can achieve such level of accuracy, and, most of all, be model-independent. Here we propose a method that can in principle achieve that; this is done by making minimal assumptions and using distance probes that are cosmology-independent: gravitational waves, redshift drift and cosmic chronometers. We discuss what kind of observations are needed in principle to achieve the desired accuracy.

The siren song of vocal fundamental frequency for romantic relationships.

Science.gov (United States)

Weusthoff, Sarah; Baucom, Brian R; Hahlweg, Kurt

2013-01-01

A multitude of factors contribute to why and how romantic relationships are formed as well as whether they ultimately succeed or fail. Drawing on evolutionary models of attraction and speech production as well as integrative models of relationship functioning, this review argues that paralinguistic cues (more specifically the fundamental frequency of the voice) that are initially a strong source of attraction also increase couples' risk for relationship failure. Conceptual similarities and differences between the multiple operationalizations and interpretations of vocal fundamental frequency are discussed and guidelines are presented for understanding both convergent and non-convergent findings. Implications for clinical practice and future research are discussed.

A gravitational-wave standard siren measurement of the Hubble constant

NARCIS (Netherlands)

Abbott, B. P.; Abbott, R.; Abbott, T. D.; Acernese, F.; Ackley, K.; Adams, C.; Adams, T.; Addesso, P.; Adhikari, R. X.; Adya, V. B.; Affeldt, C.; Afrough, M.; Agarwal, B.; Agathos, M.; Agatsuma, K.; Aggarwal, N.; Aguiar, O. D.; Aiello, L.; Ain, A.; Ajith, P.; Allen, B.; Allen, G.; Allocca, A.; Altin, P. A.; Amato, A.; Ananyeva, A.; Anderson, S. B.; Anderson, W. G.; Angelova, S. V.; Antier, S.; Appert, S.; Arai, K.; Araya, M. C.; Areeda, J. S.; Arnaud, N.; Arun, K. G.; Ascenzi, S.; Ashton, G.; Ast, M.; Aston, S. M.; Astone, P.; Atallah, D. V.; Aufmuth, P.; Aulbert, C.; AultO'Neal, K.; Austin, C.; Avila-Alvarez, A.; Babak, S.; Bacon, P.; Bader, M. K. M.; Bae, S.; Baker, P. T.; Baldaccini, F.; Ballardin, G.; Ballmer, S. W.; Banagiri, S.; Barayoga, J. C.; Barclay, S. E.; Barish, B. C.; Barker, D.; Barkett, K.; Barone, F.; Barr, B.; Barsotti, L.; Barsuglia, M.; Barta, D.; Bartlett, J.; Bartos, I.; Bassiri, R.; Basti, N.; Batch, J. C.; Bawaj, M.; Bayley, J. C.; Bazzan, M.; Becsy, B.; Beer, C.; Bejger, M.; Belahcene, I.; Bell, A. S.; Berger, B. K.; Bergmann, G.; Bero, J. J.; Berry, C. P. L.; Bersanetti, D.; Bertolini, A.; Betzwieser, J.; Bhagwat, S.; Bhandare, R.; Bilenko, I. A.; Billingsley, G.; Billman, C. R.; Birch, J.; Birney, R.; Birnholtz, O.; Biscans, S.; Biscoveanu, S.; Bisht, A.; Bitossi, M.; Biwer, C.; Bizouard, A.; Blackburn, J. K.; Blackman, J.; Blair, C. D.; Blair, D. G.; Blair, R. M.; Bloemen, S.; Bock, O.; Bode, N.; Boer, M.; Bogaert, G.; Bohe, A.; Bondu, F.; Bonilla, E.; Bonnand, R.; Boom, B. A.; Bork, R.; Boschi, V.; Bose, S.; Bossie, K.; Bouffanais, Y.; Bozzi, A.; Bradaschia, C.; Brady, P. R.; Branchesi, M.; Brau, J. E.; Briant, T.; Brillet, A.; Brinkmann, M.; Brisson, V.; Brockill, R.; Broida, J. E.; Brooks, N. F.; Brown, D. A.; Brown, D. D.; Brunett, S.; Buchanan, C. C.; Buikema, A.; Bulik, H.; Bulten, H. J.; Buonanno, A.; Buskulic, D.; Buy, C.; Byer, R. L.; Cabero, M.; Cadonati, L.; Cagnoli, G.; Cahillane, C.; Bustillo, J. Calderon; Callister, T. A.; Calloni, E.; Camp, J. B.; Canepa, M.; Canizares, P.; Cannon, K. C.; Cao, H.; Cao, J.; Capano, C. D.; Capocasa, E.; Carbognani, F.; Caride, S.; Carney, M. F.; Diaz, J. Casanueva; Casentini, C.; Caudill, S.; Cavaglia, M.; Cavalier, F.; Cavalieri, R.; Cella, G.; Cepeda, C. B.; Cerda-Duran, P.; Cerretani, G.; Cesarini, E.; Chamberlin, S. J.; Chan, M.; Chao, S.; Charlton, P.; Chase, E.; Chassande-Mottin, E.; Chatterjee, D.; Chatziioannou, K.; Cheeseboro, B. D.; Chen, H. Y.; Chen, X.; Chen, Y.; Cheng, H. -P.; Chia, H.; Chincarini, N.; Chiummo, A.; Chmiel, T.; Cho, H. S.; Cho, M.; Chow, J. H.; Christensen, N.; Chu, Q.; Chua, A. J. K.; Chua, S.; Chung, A. K. W.; Chung, S.; Ciani, G.; Ciolfi, R.; Cirelli, C. E.; Cirone, N.; Clara, F.; Clark, J. A.; Clearwater, P.; Cleva, F.; Cocchieri, C.; Coccia, E.; Cohadon, P. -F.; Cohen, D.; Colla, A.; Collette, C. G.; Cominsky, L. R.; Constancio, M., Jr.; Conti, L.; Cooper, S. J.; Corban, P.; Corbitt, T. R.; Cordero-Carrion, I.; Corley, K. R.; Cornish, N.; Corsi, A.; Cortese, S.; Costa, C. A.; Coughlin, M. W.; Coughlin, S. B.; Coulon, J. -P.; Countryman, S. T.; Couvares, P.; Covas, P. B.; Cowan, E. E.; Coward, D. M.; Cowart, M. J.; Coyne, D. C.; Coyne, R.; Creighton, J. D. E.; Creighton, T. D.; Cripe, J.; Crowder, S. G.; Cullen, T. J.; Cumming, A.; Cunningham, L.; Cuoco, E.; Dal Canton, T.; Dalya, G.; Danilishin, S. L.; D'Antonio, S.; Danzmann, K.; Dasgupta, A.; Costa, C. F. Da Silva; Datrier, L. E. H.; Dattilo, V.; Dave, I.; Davier, M.; Davis, E. J.; Daw, E. J.; Day, B.; De, S.; DeBra, D.; Degallaix, J.; De laurentis, M.; Deleglise, S.; Del Pozzo, W.; Demos, N.; Denker, T.; Dent, T.; De Pietri, R.; Dergachev, V.; De Rosa, R.; DeRosa, R. T.; De Rossi, C.; DeSalvo, R.; de Verona, O.; Devenson, J.; Dhurancihar, S.; Diaz, M. C.; Di Fiore, L.; Di Giovanni, M.; Di Girolamo, T.; Di Lieto, A.; Di Pace, S.; Di Palma, I.; Di Renzo, F.; Doctor, Z.; Dolique, V.; Donovan, F.; Dooley, K. L.; Doravari, S.; Dorrington, I.; Douglas, R.; Alvarez, M. Dovale; Downes, T. P.; Drago, M.; Dreissigacker, C.; Driggers, J. C.; Du, Z.; Ducrot, M.; Dupej, P.; Dwyer, S. E.; Edo, T. B.; Edwards, M. C.; Effler, N.; Eggenstein, H. -B.; Ehrens, P.; Eichholz, J.; Eikenberry, S. S.; Eisenstein, R. A.; Essick, R. C.; Estevez, D.; Etienne, Z. B.; Etzel, T.; Evans, M.; Evans, T. M.; Factourovich, M.; Fafone, V.; Fair, H.; Fairhurst, S.; Fan, X.; Farinon, S.; Farr, B.; Farr, W. M.; Fauchon-Jones, E. J.; Favata, M.; Fays, M.; Fee, C.; Fehrmann, H.; Feicht, J.; Fejer, M. M.; Fernandez-Galiana, A.; Ferrante, I.; Ferreira, E. C.; Ferrini, F.; Fidecaro, F.; Finstad, D.; Fiori, I.; Fiorucci, D.; Fishbach, M.; Fisher, R. P.; Fitz-Axen, M.; Flaminio, R.; Fletcher, M.; Fong, H.; Font, J. A.; Forsyth, P. W. F.; Forsyth, S. S.; Fournier, J. -D.; Frasca, S.; Frasconi, E.; Frei, Z.; Freise, A.; Frey, R.; Frey, V.; Fries, E. M.; Fritschel, P.; Frolov, V. V.; Fulda, P.; Fyffe, M.; Gabbard, H.; Gadre, B. U.; Gaebel, S. M.; Gair, J. R.; Gammaitoni, L.; Ganija, M. R.; Gaonkar, S. G.; Garcia-Quiros, C.; Garufi, F.; Gateley, B.; Gaudio, S.; Gaur, G.; Gayathril, V.; Gehrels, N.; Gemme, G.; Genin, E.; Gennai, A.; George, D.; George, J.; Gergely, L.; Germain, V.; Ghonge, S.; Ghosh, Abhirup; Ghosh, Archisman; Ghosh, S.; Giaime, J. A.; Giardina, K. D.; Giazotto, A.; Gill, K.; Glover, L.; Goetz, E.; Goetz, R.; Gomes, S.; Goncharov, B.; Gonzalez, G.; Castro, J. M. Gonzalez; Gopakumar, A.; Gorodetsky, M. L.; Gossan, S. E.; Gosselin, M.; Gouaty, R.; Grado, A.; Graef, C.; Granata, M.; Grant, A.; Gras, S.; Gray, C.; Greco, G.; Green, A. C.; Gretarsson, E. M.; Groot, P.; Grote, H.; Grunewald, S.; Gruning, P.; Guidi, G. M.; Guo, X.; Gupta, A.; Gupta, M. K.; Gushwa, K. E.; Gustafson, E. K.; Gustafson, R.; Halim, O.; Hall, B. R.; Hall, E. D.; Hamilton, E. Z.; Hammond, G.; Haney, M.; Hanke, M. M.; Hanks, J.; Hanna, C.; Hannam, M. D.; Hannuksela, O. A.; Hanson, J.; Hardwick, I.; Harms, J.; Harry, G. M.; Harry, I. W.; Hart, M. J.; Hester, C. -J.; Haughian, K.; Healy, J.; Heidmann, N.; Heintze, M. C.; Heitmann, H.; Hello, P.; Hemming, G.; Hendry, M.; Heng, I. S.; Hennig, J.; Heptonstall, A. W.; Heurs, M.; Hild, S.; Hinderer, T.; Hoak, D.; Hofman, D.; Holt, K.; Holz, D. E.; Hopkins, P.; Horst, C.; Hough, J.; Houston, E. A.; Howell, E. J.; Hreibi, A.; Hu, Y. M.; Huerta, E. A.; Huet, D.; Hughey, B.; Husa, S.; Huttner, S. H.; Huynh-Dinh, T.; Indik, N.; Inta, R.; Intini, G.; Isa, H. N.; Isac, J. -M.; Isi, M.; Iyer, B. R.; Izumi, K.; Jacqmin, T.; Jani, K.; Jaranowski, P.; Jawahar, S.; Jimenez-Forteza, F.; Johnson, W. W.; Jones, D. I.; Jones, R.; Jonker, R. J. G.; Ju, L.; Junker, J.; Kalaghatgi, C. V.; Kalogera, V.; Kamai, B.; Kandhasamy, S.; Kang, G.; Kanner, J. B.; Kapadia, S. J.; Karki, S.; Karvinen, K. S.; Kasprzack, M.; Katolik, M.; Katsavounidis, E.; Katzman, W.; Kaufer, S.; Kawabe, K.; Kefelian, E.; Keitel, D.; Kemball, A. J.; Kennedy, R.; Kent, C.; Key, J. S.; Khalili, F. Y.; Khan, I.; Khan, S.; Khan, Z.; Khazanov, E. A.; Kijbunchoo, N.; Kim, Chunglee; Kim, J. C.; Kim, K.; Kim, W.; Kim, W. S.; Kim, Y. -M.; Kimbrell, S. J.; King, E. J.; King, P. J.; Kinley-Hanlon, M.; Kirchhoff, R.; Kissel, J. S.; Kleybolte, L.; Klimenko, S.; Knowles, T. D.; Koch, P.; Koehlenbeck, S. M.; Koley, S.; Kondrashov, V.; Kontos, A.; Korobko, M.; Korth, W. Z.; Kowalska, I.; Kozak, D. B.; Kraemer, C.; Kringel, V.; Krishnan, B.; Krolak, A.; Kuehn, G.; Kumar, P.; Kumar, R.; Kumar, S.; Kuo, L.; Kutynia, A.; Kwang, S.; Lackey, B. D.; Lai, K. H.; Landry, M.; Lang, R. N.; Lange, J.; Lantz, B.; Lanza, R. K.; Lartaux-Vollard, N.; Lasky, P. D.; Laxen, M.; Lazzarini, A.; Lazzaro, C.; Leaci, P.; Leavey, S.; Lee, C. H.; Lee, H. K.; Lee, H. M.; Lee, H. W.; Lee, K.; Lehmann, J.; Lenon, A.; Leonardi, M.; Leroy, N.; Letendre, N.; Levin, Y.; Li, T. S.; Linker, S. D.; Littenberg, T. B.; Liu, J.; Liu, X.; Lo, R. K. L.; Lockerbie, N. N.; London, L. T.; Lord, J. E.; Lorenzini, M.; Loriette, V.; Lormand, M.; Losurdo, G.; Lough, J. D.; Lousto, C. O.; Lovelace, G.; Lueock, H.; Lumaca, D.; Lundgren, A. P.; Lynch, R.; Ma, Y.; Macas, R.; Macfoy, S.; Machenschalk, B.; Maclnnis, M.; Macleod, D. M.; Hernandez, I. Magana; Magana-Sandoval, F.; Zertuche, L. Magana; Magee, R. M.; Majorana, E.; Maksimovic, I.; Man, N.; Mandic, V.; Mangano, V.; Mansell, G. L.; Manske, M.; Mantovani, M.; Marchesoni, F.; Marion, F.; Marka, S.; Marka, Z.; Markakis, C.; Markosyan, A. S.; Markowitz, A.; Maros, E.; Marquina, A.; Martelli, F.; Martellini, L.; Martin, I. W.; Martin, R. M.; Martynov, D. V.; Mason, K.; Massera, E.; Masserot, A.; Massinger, T. J.; Masso-Reid, M.; Mastrogiovanni, S.; Matas, A.; Matichard, F.; Matone, L.; Mavalvala, N.; Mazumder, N.; McCarthy, R.; McClelland, D. E.; McCormick, S.; McCuller, L.; McGuire, S. C.; McIntyre, G.; McIver, J.; McManus, D. J.; McNeill, L.; McRae, T.; McWilliams, S. T.; Meacher, D.; Meadors, G. D.; Mehmet, M.; Meidam, J.; Mejuto-Villa, E.; Melatos, A.; Mendell, G.; Mercer, R. A.; Merilh, E. L.; Merzougui, M.; Meshkov, S.; Messenger, C.; Messick, C.; Metzdorff, R.; Meyers, P. M.; Miao, H.; Michel, C.; Middleton, H.; Mikhailov, E. E.; Milano, L.; Miller, A. L.; Miller, B. B.; Miller, J.; Millhouse, M.; Milovich-Goff, M. C.; Minazzoli, O.; Minenkov, Y.; Ming, J.; Mishra, C.; Mitra, S.; Mitrofanov, V. R.; Mitselmakher, G.; Mittleman, R.; Moffa, D.; Moggi, N.; Mogushi, K.; Mohan, M.; Mohapatra, S. R. P.; Montani, M.; Moore, C. J.; Moraru, D.; Moreno, G.; Morriss, S. R.; Mours, B.; Mow-Lowry, C. M.; Mueller, G.; Muir, A. W.; Mukherjee, Arunava; Mukherjee, D.; Mukherjee, S.; Mukund, N.; Mullavey, A.; Munch, J.; Muniz, E. A.; Muratore, M.; Murray, P. G.; Napier, K.; Nardecchia, I.; Naticchioni, L.; Nayak, R. K.; Neilson, J.; Nelemans, G.; Nelson, T. J. N.; Nery, M.; Neunzert, A.; Nevin, L.; Newport, J. M.; Newton, G.; Ng, K. K. Y.; Nguyen, T. T.; Nichols, D.; Nielsen, A. B.; Nissanke, S.; Nitz, A.; Noack, A.; Nocera, F.; Nolting, D.; North, C.; Nuttall, L. K.; Oberling, J.; O'Dea, G. D.; Ogin, G. H.; Oh, J. J.; Oh, S. H.; Ohme, F.; Okada, M. A.; Oliver, M.; Oppermann, P.; Oram, Richard J.; O'Reilly, B.; Ormiston, R.; Ortega, L. F.; O'Shaughnessy, R.; Ossokine, S.; Ottaway, D. J.; Overmier, H.; Owen, B. J.; Pace, A. E.; Page, J.; Page, M. A.; Pai, A.; Pai, S. A.; Palamos, J. R.; Palashov, O.; Palomba, C.; Pal-Singh, A.; Pan, Howard; Pan, Huang-Wei; Pang, B.; Pang, P. T. H.; Pankow, C.; Pannarale, F.; Pant, B. C.; Paoletti, F.; Paoli, A.; Papa, M. A.; Parida, A.; Parker, W.; Pascucci, D.; Pasqualetti, A.; Passaquieti, R.; Passuello, D.; Patil, M.; Patricelli, B.; Pearlstone, B. L.; Pedraza, M.; Pedurand, R.; Pekowsky, L.; Pele, A.; Penn, S.; Perez, C. J.; Perreca, A.; Perri, L. M.; Pfeiffer, H. P.; Phelps, M.; Piccinni, O. J.; Pichot, M.; Piergiovanni, F.; Pierro, V.; Pillant, G.; Pinard, L.; Pinto, I. M.; Pirello, M.; Pitkin, M.; Poe, M.; Poggiani, R.; Popolizio, P.; Porter, E. K.; Post, A.; Powell, J.; Prasad, J.; Pratt, J. W. W.; Pratten, G.; Predoi, V.; Prestegard, T.; Prijatelj, M.; Principe, M.; Privitera, S.; Prodi, G. A.; Prokhorov, L. G.; Puncken, O.; Punturo, M.; Puppo, P.; Puerrer, M.; Qi, H.; Quetschke, V.; Quintero, E. A.; Quitzow-James, R.; Raab, F. J.; Rabeling, D. S.; Radkins, H.; Raffai, P.; Raja, S.; Rajan, C.; Rajbhandari, B.; Rakhmanov, M.; Ramirez, K. E.; Ramos-Buades, A.; Rapagnani, P.; Raymond, V.; Razzano, M.; Read, J.; Regimbau, T.; Rei, L.; Reid, S.; Reitze, D. H.; Ren, W.; Reyes, S. D.; Ricci, F.; Ricker, P. M.; Rieger, S.; Riles, K.; Rizzo, M.; Robertson, N. A.; Robie, R.; Robinet, F.; Rocchi, A.; Rolland, L.; Rollins, J. G.; Roma, V. J.; Romano, J. D.; Romano, R.; Romel, C. L.; Romie, J. H.; Rosinska, D.; Ross, M. P.; Rowan, S.; Ruediger, A.; Ruggi, P.; Rutins, G.; Ryan, K.; Sachdev, S.; Sadecki, I.; Sadeghian, L.; Sakellariadou, M.; Salconi, L.; Saleem, M.; Salemi, F.; Samajdar, A.; Sammut, L.; Sampson, L. M.; Sanchez, E. J.; Sanchez, L. E.; Sanchis-Gual, N.; Sandberg, V.; Sanders, J. R.; Sassolas, B.; Sathyaprakash, B. S.; Saulson, P. R.; Sauter, O.; Savage, R. L.; Sawadsky, A.; Schale, P.; Scheel, M.; Schauer, J.; Schmidt, J.; Schmidt, P.; Schnabel, R.; Schofield, R. M. S.; Schoenbeck, A.; Schreiber, E.; Schuette, D.; Schulte, B. W.; Schutz, B. F.; Schwalbe, S. G.; Scott, J.; Scott, S. M.; Seidel, E.; Sellers, D.; Sengupta, A. S.; Sentenac, D.; Sequino, V.; Sergeev, A.; Shaddock, D. A.; Shaffer, T. J.; Shah, A. A.; Shahriar, M. S.; Shaner, M. 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B.; Bechtol, K.; Benoit-Levy, A.; Bertin, E.; Bridle, S. L.; Brooks, D.; Buckley-Geer, E.; Burke, D. L.; Rosell, A. Carnero; Kind, M. Carrasco; Carretero, J.; Castander, F. J.; Cunha, C. E.; D'Andrea, C. B.; da Costa, L. N.; Davis, C.; DePoy, D. L.; Desai, S.; Dietrich, J. P.; Estrada, J.; Fernandez, E.; Flaugher, B.; Fosalba, P.; Gaztanaga, E.; Gerdes, D. W.; Giannantonio, T.; Goldstein, D. A.; Gruen, D.; Gutierrez, G.; Hartley, W. G.; Honscheid, K.; Jain, B.; James, D. J.; Jeltema, T.; Johnson, M. W. G.; Kent, S.; Krause, E.; Kron, R.; Kuehn, K.; Kuhlmann, S.; Kuropatkin, N.; Lahav, O.; Lima, M.; Maia, M. A. G.; March, M.; Miller, C. J.; Miquel, R.; Neilsen, E.; Nord, B.; Ogando, R. L. C.; Plazas, A. N.; Romer, A. K.; Roodman, A.; Rykoff, E. S.; Sanchez, E.; Scarpine, V.; Schubnell, M.; Sevilla-Noarbe, I.; Smith, M.; Smith', R. C.; Suchyta, E.; Tarle, G.; Thomas, D.; Thomas, R. C.; Troxel, M. A.; Tucker, D. L.; Vikram, V.; Walker, A. R.; Weller, J.; Zhang, Y.; Haislip, J. B.; Kouprinov, V. V.; Reichart, D. E.; Tartaglia, L.; Sand, D. J.; Valenti, S.; Yang, S.; Arcavi, Iair; Hosseinzadeh, Griffin; Howell, D. Andrew; McCully, Curtis; Poznanski, Dovi; Vasylyev, Sergiy; Tanvir, N. R.; Levan, N. J.; Hjorth, J.; Cano, Z.; Copperwheat, C.; de Ugarte-Postigo, A.; Evans, P. A.; Fynbo, J. P. U.; Gonzalez-Fernandez, C.; Greiner, J.; Irwin, M.; Lyman, J.; Mandel, I.; McMahon, R.; Milvang-Jensen, B.; O'Brien, P.; Osborne, J. P.; Perley, D. A.; Pian, E.; Palazzi, E.; Rol, E.; Rosetti, S.; Rosswog, S.; Rowlinson, A.; Schulze, S.; Steeghs, D. T. H.; Thone, C. C.; Ulaczyk, K.; Watson, D.; Wiersema, K.; Lipunov, V. M.; Gorbovskoy, E.; Kornilov, V. G.; Tyurina, N.; Balanutsa, P.; Vlasenko, D.; Gorbunov, I.; Podesta, R.; Levato, H.; Saffe, C.; Buckley, D. A. H.; Budnev, N. M.; Gress, O.; Yurkov, V.; Rebolo, R.; Serra-Ricart, M.

2017-01-01

On 17 August 2017, the Advanced LIGO1 and Virgo2 detectors observed the gravitational-wave event GW170817—a strong signal from the merger of a binary neutron-star system3. Less than two seconds after the merger, a γ-ray burst (GRB 170817A) was detected within a region of the sky consistent with the

Pharmakobotanische Untersuchungen von Lavendelsorten auf dem Plattensee- Plateau

Directory of Open Access Journals (Sweden)

Tóth, Frida

2014-09-01

Full Text Available Auf dem Hof Dörgicsei Levendula Major GmbH wurden 9 Lavendelsorten (6 Sorten von Lavandula angustifolia und 3 Sorten von Lavandula x intermedia untersucht. Neben den morphologischen und Wachstumseigenschaften wurden auch Frisch- und Trockengewichte bewertet. Quantitative und qualitative Untersuchungen von den Blüten- und Ätherischöldrogen wurden auch durchgeführt. Die statistische Analyse zeigte signifikant höhere Erträge bei den Sorten L. angustifolia ’Essence Purple’ und L. x intermedia ’Edelweiss’. Gehalt und Zusammensetzung von ätherischem Öl war eindeutig bei der Sorte L. angustifolia ’Ellagance Purple’ am günstigsten.

Physiologically-induced changes in proopiomelanocortin mRNA levels in the pituitary gland of the amphibian Xenopus laevis.

Science.gov (United States)

Martens, G J; Weterings, K A; van Zoest, I D; Jenks, B G

1987-03-13

In the pars intermedia of the pituitary gland of the amphibian Xenopus laevis the level of mRNA encoding proopiomelanocortin (POMC), the precursor protein for alpha-melanophore-stimulating hormone (alpha-MSH), is shown to be dependent on physiological parameters. POMC mRNA levels in the pars intermedia of black-background-adapted Xenopus are much higher than those of white-adapted animals. These physiological changes in POMC mRNA levels are tissue-specific because they were not found in the pars distalis of the pituitary gland. Background transfer experiments revealed that modulation of POMC gene activity is much slower than changes in the secretion of alpha-MSH.

Classical monsters in new Doctor Who fan fiction

OpenAIRE

Amanda Potter

2016-01-01

Although a number of classic Doctor Who episodes featured story lines and characters drawn from Greek myth, no new Who episodes based on Greek myth appeared until seasons 5 and 6, in 2010 and 2011. These episodes featured Pandora's box, the Minotaur, and a Siren. They all use the mythical monster or artifact outside of its ancient Greek context, and I argue that the mythical monsters were additions to earlier story ideas. I compare this with the treatment of the myths of the Minotaur and the ...

France's seismic zoning

International Nuclear Information System (INIS)

Mohammadioun, B.

1997-01-01

In order to assess the seismic hazard in France in relation to nuclear plant siting, the CEA, EDF and the BRGM (Mine and Geology Bureau) have carried out a collaboration which resulted in a seismic-tectonic map of France and a data base on seismic history (SIRENE). These studies were completed with a seismic-tectonic zoning, taking into account a very long period of time, that enabled a probabilistic evaluation of the seismic hazard in France, and that may be related to adjacent country hazard maps

Cloning, functional characterization and genomic organization of 1,8-cineole synthases from Lavandula.

Science.gov (United States)

Demissie, Zerihun A; Cella, Monica A; Sarker, Lukman S; Thompson, Travis J; Rheault, Mark R; Mahmoud, Soheil S

2012-07-01

Several members of the genus Lavandula produce valuable essential oils (EOs) that are primarily constituted of the low molecular weight isoprenoids, particularly monoterpenes. We isolated over 8,000 ESTs from the glandular trichomes of L. x intermedia flowers (where bulk of the EO is synthesized) to facilitate the discovery of genes that control the biosynthesis of EO constituents. The expression profile of these ESTs in L. x intermedia and its parents L. angustifolia and L. latifolia was established using microarrays. The resulting data highlighted a differentially expressed, previously uncharacterized cDNA with strong homology to known 1,8-cineole synthase (CINS) genes. The ORF, excluding the transit peptide, of this cDNA was expressed in E. coli, purified by Ni-NTA agarose affinity chromatography and functionally characterized in vitro. The ca. 63 kDa bacterially produced recombinant protein, designated L. x intermedia CINS (LiCINS), converted geranyl diphosphate (the linear monoterpene precursor) primarily to 1,8-cineole with K ( m ) and k ( cat ) values of 5.75 μM and 8.8 × 10(-3) s(-1), respectively. The genomic DNA of CINS in the studied Lavandula species had identical exon-intron architecture and coding sequences, except for a single polymorphic nucleotide in the L. angustifolia ortholog which did not alter protein function. Additional nucleotide variations restricted to L. angustifolia introns were also observed, suggesting that LiCINS was most likely inherited from L. latifolia. The LiCINS mRNA levels paralleled the 1,8-cineole content in mature flowers of the three lavender species, and in developmental stages of L. x intermedia inflorescence indicating that the production of 1,8 cineole in Lavandula is most likely controlled through transcriptional regulation of LiCINS.

Morphological and cytochemical characterization of cell types of the adenohypophysis of Manjuba, Anchoviella lepidentostole (Fowler, 1911 (Osteichthyes, Engraulidae

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Sarah Arana

1997-04-01

Full Text Available The pituitary gland of Anchoviella lepidentostole consists of the neurohypophysis and the adenohypophysis, which is subdivided in pars intermedia and pars distalis. The pars distalis comprises pars distalis rostralis and pars distalis proximalis. The cell types of the pars distalis rostralis are arranged in follicles. In the follicular epithelium, four cell types were cytochemically characterized: l-PDR (basophilic, ll-PDR (lead haematoxylin+/HPb+, lll-PDR (PAS+, AB pH2.5+ and AF+, IV-PDR (acidophilic. Thepars distalis proximalis has two cell types: l-PDP (PAS+, AB pH 2.5+ and AF+ and ll-PDP (acidophilic. In the pars intermedia there are two cell types: l-PI (HPb+ and ll-PI (chromophobes.

[Sirenomelia apus. Case report].

Science.gov (United States)

Chávez-Corral, Dora Virginia; Aguilar Torres, Cesar R; Levario-Carrillo, Margarita; Alcalá-Sánchez, Imelda; Fierro-Murga, Ricardo; Arámbula-Almanza, Jaqueline; García-Mendoza, Alejandra

2011-08-01

We report a case of Sirenomelia. The mother began prenatal care in the second trimester. Transabdominal ultrasound was determined anhydramnios, cardiac abnormalities and lumbosacral spine. We obtained a single fetus of 21 weeks' gestation with fused lower extremities from the hip to finish in a stump without the presence of feet. Heart with transposition of the great vessels, among other birth defects. It was classified as symelia, Apodi apus, monopodio sirenoide, siren ectropodia, type VI. It is important to diagnose early, because it is a serious and deadly disorder.

Las sirenas, o la inmortalidad de un mito (una visión comparatista)

OpenAIRE

Pedrosa, José Manuel

2015-01-01

Resumen: Las sirenas son uno de los mitos más fascinantes y perdurables de los que pueblan el imaginario humano. Este artículo revisa su cronología y su geografía, desde los orígenes hasta hoy. Y estudia sus versiones en la literatura oral y en la literatura escrita. Abstract: Sirens and mermaids are one of the most fascinating and enduring of the myths that live in human imagination. This article reviews its chronology and geography, from its origins to today. And it studies v...

Using a new, free spectrograph program to critically investigate acoustics

Science.gov (United States)

Ball, Edward; Ruiz, Michael J.

2016-11-01

We have developed an online spectrograph program with a bank of over 30 audio clips to visualise a variety of sounds. Our audio library includes everyday sounds such as speech, singing, musical instruments, birds, a baby, cat, dog, sirens, a jet, thunder, and screaming. We provide a link to a video of the sound sources superimposed with their respective spectrograms in real time. Readers can use our spectrograph program to view our library, open their own desktop audio files, and use the program in real time with a computer microphone.

Análise de custos da atenção hospitalar a recém-nascidos de risco: uma comparação entre Unidade Intermediária Convencional e Unidade Canguru Análisis de los costes de la atención hospitalaria para los recién nacidos en riesgo: una comparación entre la Unidad Intermedia Neonatal y la Unidad Canguro Cost analysis of hospital care for newborns at risk: comparison of an Intermediate Neonatal Care Unit and a Kangaroo Unit

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Cynthia Magluta

2013-06-01

Full Text Available O objetivo deste estudo foi realizar uma análise comparativa entre o custo da Unidade Canguru e da Unidade Intermediária Convencional sob a perspectiva do SUS, no Município do Rio de Janeiro, Brasil. A população de referência é de recém-nascidos estáveis clinicamente, que podem receber assistência nas duas modalidades de cuidado. Um modelo de análise de decisão para uma coorte hipotética de mil recém-nascidos elegíveis foi elaborado para comparar os custos em cada estratégia avaliada. As probabilidades dos eventos e o consumo de recursos de saúde foram incorporados ao modelo com base na literatura e consulta a especialistas. O custo da diária foi de R$343,53 para a 2ª etapa da Unidade Canguru e de R$394,22 para a Unidade Intermediária Convencional. O custo para a coorte hipotética foi de R$5.710.281,66 para a assistência na 2ª e 3ª etapa da Unidade Canguru e de R$7.119.865,61 para a Unidade Intermediária Convencional. A Unidade Intermediária Convencional apresentou custos 25% superiores aos da Unidade Canguru. O estudo pode contribuir para a tomada de decisão na área da saúde, além de fornecer subsídios para pesquisas relacionadas à avaliação econômica na área neonatal.El objetivo de este estudio fue realizar un análisis comparativo entre el coste de la Unidad Canguro y la Unidad Intermedia Convencional, dentro de la perspectiva del Sistema Único de Salud (SUS en el municipio de Río de Janeiro, Brasil. La población de referencia son los recién nacidos clínicamente estables, que pueden recibir asistencia en ambas modalidades de atención. Se diseñó un modelo de análisis de decisión para una cohorte hipotética de 1.000 bebés elegibles, con el fin de comparar los costes de cada estrategia evaluada. Las probabilidades de ocurrencias y consumo de recursos sanitarios se incorporaron al modelo de la literatura y la consulta con expertos. El coste diario fue de R$343,53 en la 2ª etapa de la Unidad Canguro

Xmni polymorphism and disease severity in patients with beta thalassemia from northern Pakistan

International Nuclear Information System (INIS)

Hanif, T.B.; Ahmed, S.; Anwar, J.

2015-01-01

Thalassemia is a heterogeneous disorder and several genetic factors influence the severity of thalassemia. An accurate and early diagnosis of a mild thalassemia genotype helps to avoid unnecessary transfusion and its complications. The aim of this study is to identify the association between XmnI polymorphism and disease severity in patients with ?-thalassemia from northern Pakistan. Methods: The cross sectional study was conducted at the Department of Haematology, Armed Forces Institute of Pathology (AFIP) Rawalpindi, from September 2006 to June 2009. A total of 90 subjects including 30 with thalassemia major, 30 with thalassemia intermedia and 30 normal individuals were studied. DNA from each subject was tested for 15 ?-thalassemia mutations and the presence of XmnI polymorphism using Amplification Refractory Mutation System and Restriction Fragment Length Polymorphism respectively. Results: One normal and one thalassemia major subject were found to be positive for homozygous and heterozygous XmnI polymorphism respectively. Among the thalassemia intermedia group, XmnI polymorphism was found in 12/30 patients, of whom 10 were homozygous and 2 were heterozygous for it. Conclusion: XmnI polymorphism is an important genotypic factor in Pakistani population for making a prospective diagnosis of thalassemia intermedia and predicting the severity of the disease. (author)

Leishmaniose tegumentar americana na Ilha Grande, Rio de Janeiro: VI. observações sobre a freqüência horária e variação mensal dos transmissores

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Nelson A. de Araújo Filho

1981-12-01

Full Text Available Para estudo da freqüência horária dos principais vetores da Leishmaniose Tegumentar Americana (LTA, na Ilha Grande, durante os anos de 1976 a 1977, foram realizadas 4 capturas de 24 horas com isca animal (cão, ao ar livre, e 5 capturas de 24 horas em um único domicilio da área. Observou-se que a espécie Lutzomyia intermedia ocorreu durante todo o período noturno, com densidade constante no domicílio humano, enquanto a espécie Lutzomyia migonei apresentou maiores densidades no período da madrugada, nas capturas com isca animal (cão. Em 156 horas de estudo sobre variação mensal, durante o período de março de 1976 a fevereiro de 1977, a espécie L. intermedia ocorreu durante todo o ano, sendo abril, maio, outubro e dezembro os meses de maior densidade; a espécie L. migonei apresentou-se com baixa densidade, chegando a desaparecer no mês de setembro. As elevadas densidades das espécies L. intermedia e L. migonei indicam-nas como as prováveis vetoras de LTA na Ilha Grande.

On variation in Schloenbachia varians (J. Sowerby, 1817) from the Lower Cenomanian of western Kazakhstan

Science.gov (United States)

Kennedy, William James

2013-12-01

Kennedy, W.J. 2013. On variation in Schloenbachia varians (J. Sowerby, 1817) from the Lower Cenomanian of western Kazakhstan Acta Geologica Polonica, 63(4), 443-468. Warszawa. An assemblage of 94 specimens of Schloenbachia varians (J. Sowerby, 1817) from the Lower Cenomanian Sharpeiceras schlueteri Subzone of the Mantelliceras mantelli Zone of the Besakty section in the Mangyshlak Mountains of western Kazakhstan includes 26 complete adults that range from to 59-174 mm in diameter. No size-related dimorphism was detected in the assemblage, which shows wide, continuous intraspecific variation. This is described in terms of five formae; from robust to gracile these are: ventriosa, varians sensu stricto, subtuberculata , intermedia, and subplana. The ratio of robust (ventriosa + varians sensu stricto) to gracile (subtuberculata + intermedia + subplana) individuals is 34% to 66%. The reference specimens of the formae and their synonyms are described and illustrated, and related to the Besakty material. The modification of adult body chamber ornament of all formae is documented, and Jakeiceras Cooper and Owen, 2011 is shown to be based on an adult of a passage form between forma subtuberculata and forma intermedia. The differences between Lower Cenomanian S. varians, lower Middle Cenomanian S. coupei (Brongniart, 1822), and upper Middle and lower Upper Cenomanian S. lymensis Spath, 1926b are described and illustrated.

Community Based Warning and Evacuation System against Debris Flow in the Upper Jeneberang River, Gowa, South Sulawesi

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Sutikno Hardjosuwarno

2008-07-01

Full Text Available Gigantic collapse of the Caldera wall of Mt. Bawakaraeng (2,830 m in March 2004 had supplied the sediment volume of 230 million to the most upper stream of Jeneberang River, which flowed down to the lower reach in the form of debris flow which is triggered by rainfall. The purpose of the research is to provide a system which is able to forecast the occurrence of debris flow, to identify the weak points along the river course, to identify the hazard areas and how to inform effectively and efficiently the warning messages to the inhabitants in the dangerous area by using the existing modern equipment combined with the traditional one. The standard rainfall which is used to judge the occurrence of debris flow was established by Yano method. It is based on the historical data of rainfall that trigger and not trigger to the occurrence of debris flow which is widely used in Japan so far. The hazard area was estimated by Two-Dimensional Simulation Model for debris flow, the debris flow arrival time at each point in the river were estimated by dividing their distance from reference point by debris flow velocity, where the check dam no. 7-1 in Manimbahoi was designated as reference point. The existing evacuation routes were checked by field survey, the strength and coverage of sound for kentongan and manual siren were examined using sound pressure level at the location of the existing monitoring post and the effectiveness of warning and evacuation were evaluated by comparing the warning and evacuation time against the debris flow arrival time. It was resulted that debris flow occurrence was triggered by short duration of high rainfall intensity, long duration of low rainfall intensity and the outbreak of natural dam which is formed by land slide or bank collapses. The hazard area of upper Jeneberang River are mostly located on the river terraces where the local inhabitants earn their living through cultivating the river terraces as paddy fields, dry

Emergency response guide for Point Lepreau area residents

International Nuclear Information System (INIS)

1982-01-01

The design, construction and operating procedures of CANDU nuclear generating stations ensure that an accident causing a significant risk to people living near these stations is extremely unlikely. However, despite the excellent safety record of nuclear stations, it is common practice to prepare an emergency plan for such facilities. In this regard, The New Brunswick Emergency Measures Organization is responsible for developing and implementing the Off-Site Emergency Plan for the Point Lepreau Generating Station. Work for the Off-Site Emergency Plan began in 1976 and, under the leadership of N.B.E.M.O., a number of government agencies co-operated in this project. The completed plan thus represents agreement among a number of Province of New Brunswick departments, various community groups, NB Power, and representatives of the Government of Canada. Also, information gathered in the annual door-to-door survey of the Lepreau area enabled government planners to make specialized arrangements such as an extensive warden service, a siren system, and evacuation assistance for the disabled

The malacostraca Caspian Sea as object of acclimatization and enrichment of a foоd reserve of fishes of lakes, water basins of foothill and mountain Dagestan

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A. A. Abdulmedzhidov

2009-01-01

Full Text Available Peracarilae – Paramysis (Paramysis intermedia, Paramysis (Paramysis kessleri, Paramysis (Mesomysis lacustris, Limnomysis benedeni, Niphargoides (Pontogammarus robustoides, can live in mountain lakes of Dagestan.

Flood warnings in coastal areas: how do social and behavioural patterns influence alert services?

Science.gov (United States)

Pescaroli, G.; Magni, M.

2015-01-01

Many studies discuss the economic and technical aspects of flood warnings. Less attention has been given to the social and psychological patterns that affect alert services. In particular, the literature focuses on warnings activated in river basins or marine environments without providing clear evidence of relevance to Mediterranean coastal areas, even though these are subjected to growing flood risk related to climate change. This paper is a first attempt to bridge this gap. Our research develops an in- depth analysis of the village of Cesenatico on the Adriatic Sea coast. Here the municipality adopted two complementary warning systems: a siren and an alert via Short Message Service (SMS). The analysis focuses on a survey conducted in 2011 and 2012 with 228 participants. The relationships between social and behavioural variables and warning services are investigated, and so are flood preparedness and information dissemination. Qualitative evidence from informal interviews is used to support the understanding of key responses. The conclusions show how different social and behavioural patterns can influence the effectiveness and use of warning systems, regardless of the technology adopted and the structural mitigation measures implemented. Education, training and accountability are seen to be critical elements for improvement. Finally, the statistical output is used to suggest new questions and new directions for research.

Identification of Glucosinolates in Seeds of Three Brassicaceae Species Known to Hyperaccumulate Heavy Metals.

Science.gov (United States)

Montaut, Sabine; Guido, Benjamin S; Grison, Claude; Rollin, Patrick

2017-03-01

Plants from the Brassicaceae family are known to contain secondary metabolites called glucosinolates. Our goal was to establish by LC/MS the glucosinolate profile of seeds of three Brassicaceae species known to hyperaccumulate heavy metals. We investigated Alyssum fallacinum auct. non Hausskn., Iberis intermedia Guers., and Noccaea caerulescens (J. Presl & C. Presl) F. K. Mey. Our results indicate that A. fallacinum seeds contain glucoiberin and glucoibervirin, which had not been previously identified in this plant. Furthermore, we report for the first time the presence of glucoiberin, glucoibervirin, glucotropaeolin, and sinigrin in I. intermedia. We have detected for the first time glucoconringiin in N. caerulescens. In addition, glucosinalbin, 4-hydroxyglucobrassicin, and glucomoringin were also detected. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

Chillán, una ciudad morfológica y socialmente fragmentada en busca del desarrollo sustentable

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Nelson Anabalón Pinto

2006-05-01

Full Text Available Chillán, una ciudad intermedia, se ubica en la depresión intermedia de Chile Central, a poco más de cien kilómetros de Concepción, capital de la región del Biobío, y a cuatrocientos kilómetros de la ciudad de Santiago. Es el centro funcional y espacial de la provincia de Ñuble donde los habitantes de las comunas rurales convergen para satisfacer sus necesidades de servicio y equipamiento. La población comunal asciende a 156.537 habitantes, de los cuales el 88% se concentra en la ciudad de Chillán y un 12% en las zonas rurales de la comuna.

Distribution, feeding and ecomorphology of four species of Auchenipteridae (Teleostei: Siluriformes in Eastern Amazonia, Brazil

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Tiago M. S. Freitas

Full Text Available ABSTRACT Fish exhibit morphological, physiological and behavioral specializations which enable them to display different ways to explore the environments and resources. Thus, the aim of this study was to verify how four Auchenipteridae species differ in the distribution, feeding habits and morphological traits: Auchenipterichthys longimanus (Günther, 1864, Auchenipterus nuchalis (Spix & Agassiz, 1829, Tatia intermedia (Steindachner, 1877 and Trachelyopterus galeatus (Linnaeus, 1766. This study was conducted in rivers and bays of the Anapú Basin, Pará State (Brazil, where these species are abundant. Specimens were collected using gillnets, and after caught the stomachs were removed for the contents analyzes. Eighteen morphometric measurements from ten adult specimens of each species were taken, combined into fifteen ecomorphological attributes. The species distribution showed that A. longimanus was restricted to rivers, while the others were exclusively caught in the bays. All four species had their diet composed of allochthonous insects, but A. longimanus also exhibited a great frugivorous habit. The most important ecomorphological attributes were relative to the consumption of larger food items (for A. longimanus and T. galeatus and to the longer swimming capacity (for A. longimanus and T. intermedia. These morphological differences and the trophic diversity presented in this study highlighted some important information about how ecomorphological similar species behave and share resources, which may play a significant role on the coexistence of these species in the Anapú Basin.

Methodological aspects of mutation breeding

International Nuclear Information System (INIS)

Broertjes, C.; Roest, S.; Bokelmann, G.S.

1980-01-01

The authors discuss the development of the in vitro adventitious bud technique for Chrysanthemum morifolium, Solanum tuberosum, Begonia x hieemalis, Dianthus caryophyllus, Muscari, Euphorbia pulcherrima, and Forsythia intermedia. (G.T.H.)

Arabidopsis CDS blastp result: AK061856 [KOME

Lifescience Database Archive (English)

Full Text Available rotein similar to stem secoisolariciresinol dehydrogenase GI:13752458 from {Forsythia x intermedia}; similar to sex determination protein tasselseed 2 SP:P50160 from [Zea mays] 4e-65 ...

Arabidopsis CDS blastp result: AK070585 [KOME

Lifescience Database Archive (English)

Full Text Available otein similar to stem secoisolariciresinol dehydrogenase GI:13752458 from {Forsythia x intermedia}; similar to sex determination protein tasselseed 2 SP:P50160 from [Zea mays] 2e-67 ...

Arabidopsis CDS blastp result: AK104614 [KOME

Lifescience Database Archive (English)

Full Text Available rotein similar to stem secoisolariciresinol dehydrogenase GI:13752458 from {Forsythia x intermedia}; similar to sex determination protein tasselseed 2 SP:P50160 from [Zea mays] 2e-67 ...

Arabidopsis CDS blastp result: AK107619 [KOME

Lifescience Database Archive (English)

Full Text Available rotein similar to stem secoisolariciresinol dehydrogenase GI:13752458 from {Forsythia x intermedia}; similar to sex determination protein tasselseed 2 SP:P50160 from [Zea mays] 2e-67 ...

SiRen: Leveraging Similar Regions for Efficient and Accurate Variant Calling

Science.gov (United States)

2015-05-30

Cloudera, EMC2, Ericsson, Facebook, Guavus, HP, Huawei, Informatica , Intel, Microsoft, NetApp, Pivotal, Samsung, Schlumberger, Splunk, Virdata and VMware...EMC2, Ericsson, Facebook, Guavus, HP, Huawei, Informatica , Intel, Microsoft, NetApp, Pivotal, Samsung, Schlumberger, Splunk, Virdata and VMware

The Siren Song of Economic Diversification: Alberta’s Legacy of Loss

Directory of Open Access Journals (Sweden)

F.L. Ted Morton

2015-03-01

Full Text Available Former Alberta premier Peter Lougheed is celebrated for his defence of the province and Western Canada during the energy wars of the 1970s, and deservedly so. Prime Minister Pierre Trudeau was a formidable opponent. He was able and willing to use the full arsenal of federal powers to redirect soaring western energy revenues away from Alberta to Ottawa. For those of us in Western Canada, it is unpleasant to imagine what the outcome of this struggle would have been if a lesser man than Peter Lougheed had been at Alberta’s helm. But there is another aspect of the Lougheed legacy that is less remembered because it is less celebrated—also deservedly so. These were Lougheed’s ambitious economic diversification projects. Between 1973 and 1993 (when Ralph Klein became premier, the Lougheed-Getty “forced-growth” economic diversification projects are conservatively estimated to have cost Albertans $2.2 billion. While former premier Don Getty got most the blame for these losses (as many occurred during his watch, most of these programs began earlier. Lougheed’s push for government-led diversification of the Alberta economy was a policy hallmark of his 1971 electoral breakthrough, and marked a sharp break from three decades of Social Credit laissez-faire policies. The Lougheed-Getty diversification fiascos are of more than just historical interest. While the subsequent Progressive Conservative (PC regime of Premier Ralph Klein (1993–2006 followed an explicit philosophy of “government is not in the business of business,” the more recent Stelmach (2006–11 and Redford (2011-14 governments have not. Both have embraced government-sponsored “value-added” and diversification initiatives, including the North West Redwater Partnership upgrader and two new endowments to provide “funding for social and cultural innovation, and agricultural innovation.”† As Alberta’s fifth premier in the past nine years, Jim Prentice, takes the helm and tries to restore some stability to Alberta’s public finances, it merits revisiting the Lougheed-Getty experience for lessons learned. Our read of their record cautions against going down the same road again. While we identify several successes (e.g., Syncrude, Alberta Energy Company, and the ethane-based petrochemical industry, these were mostly in the hydrocarbon energy sector, and so contributed little to diversifying Alberta’s economy. Our analysis identifies the largest dollar losses (the “Dirty Dozen”, several of which suggest that failure to control costs is endemic to government-led projects. Last but not least, the sheer number and diversity of government-funded projects reflects an unhealthy culture of corporate cronyism. With billions of dollars sitting in the newly created Alberta Heritage Savings Trust Fund earmarked for “diversification” and “capital projects,” the temptation to spend became irresistible. The Heritage Fund, rather than serving its original purpose of a long-term “rainy-day account,” became a giant slush fund for ministers’ pet projects. The result is that, in real dollars, the Heritage Savings Trust Fund has a lower net worth in 2015 than it did in 1987. By the time Klein won the leadership of the PCs in 1993, his predecessors had racked up over $23 billion in net debt. Klein is widely celebrated by some (and criticized by others for the harsh budget cuts he made to eliminate the structural deficit he inherited. Less well known is that the Klein team also terminated almost all the Lougheed-Getty diversification and stimulus programs. In their stead, the Klein governments—under the leadership of treasurers Jim Dinning and Stockwell Day—pursued a diversification policy based on macroeconomics: making Alberta the most taxcompetitive jurisdiction in Canada. This “build-it-and-they-will-come” approach was intended to attract both financial and human capital. This approach has enjoyed modest success thus far, as witnessed in the growth of the financial services sector and the relocation of many corporate head offices to Calgary. It is clearly a lower-risk path to sustained prosperity than the ill-fated, government-led “forced-growth” initiatives of the Lougheed-Getty era.

Plant Guide: Limestone hawksbeard: Crepis intermedia

Science.gov (United States)

L. St. John; D. Tilley

2012-01-01

Sunflower family (Asteraceae). Limestone hawksbeard is a native perennial forb with one or two stems arising from a taproot. Plants are 30-70cm tall and basal leaves are 10-40 cm long, pinnatifid, with a fairly broad, undivided midstrip and entire or dentate segments. Plants are densely or sparsely gray-tomentulose. There are 10-60 heads per plant that are 7-12...

78 FR 16288 - Endangered and Threatened Wildlife and Plants; Permit Applications

Science.gov (United States)

2013-03-14

...--Hemistena lata Pink mucket--Lampsilis abrupta Higgins eye pearlymussel--Lampsilis higginsii Scaleshell... obliquata perobliqua Higgins eye (pearlymussel)--Lampsilis higginsii Mapleleaf, winged--Quadrula fragosa... Moccasinshell, Ochlockonee--Medionidus simpsonianus Monkeyface, Cumberland (pearlymussel)--Quadrula intermedia...

Information Space, Information Field, Information Environment

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Victor Ya. Tsvetkov

2014-08-01

Full Text Available The article analyzes information space, information field and information environment; shows that information space can be natural and artificial; information field is substantive and processual object and articulates the space property; information environment is concerned with some object and acts as the surrounding in relation to it and is considered with regard to it. It enables to define information environment as a subset of information space. It defines its passive description. Information environment can also be defined as a subset of information field. It corresponds to its active description.

Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis

Directory of Open Access Journals (Sweden)

Ana Carolina Borsanelli

2015-10-01

Full Text Available Abstratc: Bovine periodontitis is a progressive purulent infectious process associated with the presence of strictly and facultative anaerobic subgingival biofilm and epidemiologically related to soil management in large geographic areas of Brazil. This study aimed to detect species of the genera Porphyromonas and Prevotella, which occurr in periodontal pockets of cattle with lesions deeper than 5mm (n=26 and in gingival sulcus of animals considered periodontally healthy (n=25. Presence of the microorganisms was evaluated by independent-culture medium diagnostic method, using polymerase chain reaction (PCR with specific primers of Porphyromonas asaccharolytica, P. endodontalis, P. gingivalis, P. gulae, Prevotella buccae, P. intermedia, P. loescheii, P. melaninogenica, P. nigrescens, P. oralis and P. tannerae. The species P. endodontalis (80.7%, P. melaninogenica (73.1% and P. intermedia (61.5% were the most predominant in samples of cattle with periodontitis. Regarding non-injured gingival sulcus of cattle, P. endodontalis (40% and P. loeschei (40% prevailed. Porphyromonas gingivalis, P. gulae and Prevotella tannerae were not detected in the 51 samples studied. Data evaluation by T test, enabled to verify that ocorrence of Porphyromonas asaccharolytica (p=0.000003, P. endodontalis (p=0.0023, Prevotella buccae (p=0.0017, P. intermedia (p=0.0020, P. melaninogenica (p=0.00006 and P. oralis (p=0.0028 is correlated with bovine periodontitis.

Ethnopharmacological study of two Lippia species from Oriximiná, Brazil.

Science.gov (United States)

Oliveira, Danilo R; Leitão, Gilda G; Santos, Stela S; Bizzo, Humberto R; Lopes, Daíse; Alviano, Celuta S; Alviano, Daniela S; Leitão, Suzana G

2006-11-03

Lippia alba (Miller) N.E. Brown is an aromatic herb that occurs in practically all regions of Brazil and has a large importance in Brazilian folk medicine. This species is characterized by a variability in morphology and in the chemical composition of the essential oil. The present work focused on the ethnopharmacological investigation of Lippia alba, locally known as "erva-cidreira" and Lippia alba f. intermedia, known as "carmelitana". In addition, the chemical composition and antimicrobial activity of their essential oils was investigated in order to correlate to their traditional uses. The ethnopharmacological study showed a good agreement of the major use (MUA) of Lippia alba (MUA=92.0%) and to a lesser extent, for Lippia alba f. intermedia (MUA=66.7%), as sedatives. The analyses of the essential oils allowed the identification of Lippia alba as a myrcene-citral chemotype (15% and 37.1%, respectively) and Lippia alba f. intermedia as a citral chemotype (22.1%). The essential oils of both species were active against all microorganisms assayed (bacteria and fungi) by the drop test, with inhibition halos ranging from 1.1 to 5.0 cm, probably due to the high content of oxygenated monoterpenes (51.0% and 40.1%, respectively), specially represented by aldehydes and alcohols. The chemical and pharmacological data of Lippia alba are in agreement with the ethnobotanical survey.

Comparative evaluation of subgingival plaque microflora in pregnant and non-pregnant women: A clinical and microbiologic study

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Rishi Emmatty

2013-01-01

Full Text Available Context: Gingival changes in pregnancy have been attributed to changes in the subgingival biofilm related to hormonal variations. Aims: To evaluate the subgingival plaque microflora in pregnant and nonpregnant women to determine if pregnancy induces any alterations in the subgingival plaque and to associate these changes with changes in periodontal status. Settings and Design: Thirty pregnant and 10 nonpregnant women within the age group of 20-35 years having a probing pocket depth (PPD of 3-4 mm were included in the study. The pregnant women were equally divided into 3 groups of 10, each belonging to I, II, and III trimester. Materials and Methods: Plaque index, gingival index, PPD, and microbiologic evaluation for specific bacterial counts for Prevotella intermedia, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Parvimonas micra, and Fusobacterium nucleatum were carried out for all subjects. Statistical Analysis: Mann-Whitney U test. Results: Increase in gingival inflammation was observed in II and III trimester as compared with I trimester and control. Plaque scores did not show any significant difference between pregnant and nonpregnant women. Specific bacterial examination revealed an increase in proportion of P. intermedia in pregnant women of both II and III trimester as compared with I trimester and nonpregnant women. Conclusions: A definite increase in proportions of P. intermedia occurs in subgingival plaque microflora in pregnancy that may be responsible for the exaggerated gingival response.

EFFECT OF CIS ACTING POTENTIAL REGULATORS IN THE ß GLOBIN GENE CLUSTER ON THE PRODUCTION OF HBF IN THALASSEMIA PATIENTS

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Anita Nadkarni

2013-02-01

Full Text Available The clinical presentation of   b-thalassemia intermedia phenotypes are influenced by many factors .The persistence of fetal hemoglobin and  several polymorphisms located in the promoters of  g- and b-globin genes are some of them .The aim of this study was to evaluate the combined effect of  the -158Gg (CàT polymorphism and of the (ATx(Ty configuration, as well as their eventual association with elevated levels of HbF  in  b-thalassemia carriers, b-thalassemia Intermedia , b-thalassemia major and normal controls of Indian origin. The -158 Gg T allele was found to be associated with increased levels of HbF in b-thalassemia carriers, and not in wild-type subjects. In the homozygous group the -158 Gg T allele was significantly higher in the thalassemia intermedia group (66% as against the thalassemia major group (21%. The (AT9(T5 allele did not show any association with raised HbF levels. However 24% of milder cases showed presence of this allele. This study suggests that two regions of the b globin cluster, whether in cis or in trans to each other, can interact to enhance HbF expression when a b thalassemic determinant is present in heterozigosity and help in amelioration of the severity of the disease in homozygotes.

A study of membrane protein defects and alpha hemoglobin chains of red blood cells in human beta thalassemia

International Nuclear Information System (INIS)

Rouyer-Fessard, P.; Garel, M.C.; Domenget, C.; Guetarni, D.; Bachir, D.; Colonna, P.; Beuzard, Y.

1989-01-01

The soluble pool of alpha hemoglobin chains present in blood or bone marrow cells was measured with a new affinity method using a specific probe, beta A hemoglobin chain labeled with [ 3 H]N-ethylmaleimide. This pool of soluble alpha chains was 0.067 ± 0.017% of hemoglobin in blood of normal adult, 0.11 ± 0.03% in heterozygous beta thalassemia and ranged from 0.26 to 1.30% in homozygous beta thalassemia intermedia. This elevated pool of soluble alpha chains observed in human beta thalassemia intermedia decreased 33-fold from a value of 10% of total hemoglobin in bone marrow cells to 0.3% in the most dense red blood cells. The amount of insoluble alpha chains was measured by using the polyacrylamide gel electrophoresis in urea and Triton X-100. In beta thalassemia intermedia the amount of insoluble alpha chains was correlated with the decreased spectrin content of red cell membrane and was associated with a decrease in ankyrin and with other abnormalities of the electrophoretic pattern of membrane proteins. The loss and topology of the reactive thiol groups of membrane proteins was determined by using [ 3 H]N-ethylmaleimide added to membrane ghosts prior to urea and Triton X-100 electrophoresis. Spectrin and ankyrin were the major proteins with the most important decrease of thiol groups

Preliminary investigation into aerosol mobilization resulting from fusion reactor disruptions

International Nuclear Information System (INIS)

Sharpe, J.P.; Bourham, M.A.; Gilligan, J.G.

1996-01-01

An experimental system has been developed to study disruption-induced aerosol mobilization for fusion accident analysis. The SIRENS high heat flux facility at North Carolina State University has been modified to closely simulate disruption conditions expected in tokamak reactors. A hot vapor is formed by an ablation-controlled arc and expansion cooled into a glass chamber, where particle condensation and growth occur. The particles are collected and analyzed for relevant transport properties (e.g. size distribution and shape). Particle characterization methods are discussed, and preliminary results based on simple analysis techniques are given. 2 refs., 6 figs

Integrated Baseline System (IBS) Version 2.0: Models guide

Energy Technology Data Exchange (ETDEWEB)

1994-03-01

The Integrated Baseline System (IBS) is an emergency management planning and analysis tool being developed under the direction of the US Army Nuclear and Chemical Agency. This Models Guide summarizes the IBS use of several computer models for predicting the results of emergency situations. These include models for predicting dispersion/doses of airborne contaminants, traffic evacuation, explosion effects, heat radiation from a fire, and siren sound transmission. The guide references additional technical documentation on the models when such documentation is available from other sources. The audience for this manual is chiefly emergency management planners and analysts, but also data managers and system managers.

Protection of G2 and G3 against CO2

International Nuclear Information System (INIS)

Chassany, J.Ph.; Rodier, J.

1961-01-01

The presence of 60.000 m 3 of CO 2 at 15 kg/cm 2 pressure has made necessary to set up a detection and protection system on a scale equal to that used for ionising radiations. Instruments to check CO and CO 2 in the atmosphere carry out measurements continuously, alarm systems give warning if the CO 2 content increases, and the working areas may be surveyed by a whole series of portable instruments. The order for evacuation is given by sirens, and respiratory units are placed at strategic points along the exit paths. (author) [fr

Lake sediment cores as indicators of historical metal(loid) accumulation – A case study in Mexico

International Nuclear Information System (INIS)

Hansen, Anne M.

2012-01-01

To examine and compare historical accumulation of metal(loid)s in Mexican lakes and reservoirs, 210 Pb and 137 Cs dated sediment cores were evaluated: two from the remote Zempoala and Miramar Lagoons and three from Lake Pátzcuaro, and the Intermedia and Silva dams that are affected by human activities. Sediment ecotoxicology was assessed using consensus-based sediment quality guidelines for freshwater ecosystems. The +100 a sediment core from the remote Miramar Lagoon had the highest concentrations of Cr and Ni these being higher than the Probable Effect Levels (PELs). Zinc concentrations were also higher in the Miramar Lagoon compared to the other lakes and reservoirs, with concentrations higher than the Threshold Effect Level (TEL). Mercury concentrations from this lagoon were comparable to those for the Intermedia dam that receives water from urban, industrial and agricultural areas. The higher metal concentrations in the core from the Miramar Lagoon suggest that metal concentrations in the rocks of the watershed are high. Another explanation for the higher metal concentrations is the slow sediment accumulation that causes metals to be accumulated over longer time-periods at the sediment–water interface. A decrease in the concentration of As in the Intermedia dam was observed in sediments corresponding to the last decades. This may be due to an increase in sediment accumulation rate or to the reduction in sources of this metalloid in the watershed. In the Miramar Lagoon, an increase was observed in concentrations of As and Cr in more recent sediments, probably related to increased deforestation in the area or the eruption of El Chichonal volcano in 1982. Concentrations of Pb showed a decreasing tendency over the past decades in the Lake Pátzcuaro, Miramar and Zempoala Lagoons sediment cores while such behavior was not be observed for the Intermedia dam. This reduction in concentrations of Pb was attributed to the decrease in use of leaded gasoline.

Leishmaniose tegumentar americana: flebotomíneos de área de transmissão, no município de Pedro de Toledo, região sul do Estado de São Paulo, Brasil American tegumentary leishmaniasis: phlebotominae of transmission area in the Pedro de Toledo County, in the South region of São Paulo State, Brazil

Directory of Open Access Journals (Sweden)

Maria de Fátima Domingos

1998-10-01

Full Text Available Considerado como área endêmica de leishmaniose tegumentar americana (LTA, o Vale do Ribeira, na região sul do Estado de São Paulo, teve 929 casos notificados nos últimos 15 anos. Com o objetivo de identificar a fauna flebotomínea, foram realizadas capturas quinzenais em área rural do município de Pedro de Toledo, durante um ano, a partir de maio de 1994. Foram utilizadas armadilhas de Shannon no peridomicílio e armadilhas luminosas, tipo CDC, nos ambientes: domiciliar (intra e peri e florestal (margem e interior, instaladas a partir do crepúsculo vespertino. Foram coletadas 8 espécies de flebotomíneos, totalizando 11.096 exemplares, sendo Lutzomyia intermedia a espécie dominante (96,4%. Esta espécie mostrou-se mais freqüente na primeira metade da noite, ocorrendo o ano inteiro, preferencialmente no ambiente domiciliar, o que indica sua preferência por ambiente antrópico. Os dados do presente trabalho ratificam L. intermedia como importante espécie vetora de LTA no Vale do Ribeira.Considered as an American tegumentary leishmaniasis (ATL endemic area, Ribeira Valley, in the south region of São Paulo State, presented 929 notified cases in the last 15 years. Aiming to identify the phlebotomine fauna, captures were performed fortnightly in the rural area of Pedro de Toledo County during one year, from may 1994 onwards. Set at dusk Shannon traps were used in the peridomicile and CDC light traps both intra and peridomiciliarly and at the edge and in the forest. Eight species summing to 11096 specimens were caught, L. intermedia being the dominant one (96,4%. More frequent all over the year during the first half of the night preferably in the domicile surroundings this species clearly indicated its preference for the anthropic environment. The data presented in this paper ratify L. intermedia as an important ATL vector species in the Ribeira Valley.

Evaluating the fate of organic compounds in the Cameroon ...

African Journals Online (AJOL)

ISHIOMA

A level III fugacity model was developed to evaluate the fate of chemicals in the Cameroon ... environment, quantify intermedia transfer processes and the major loss ... perform baseline exposure and risk assessment of chemicals used in ...

Análisis de género de las percepciones y actitudes de los y las profesionales de atención primaria ante el cuidado informal Gender analysis of primary care professionals' perceptions and attitudes to informal care

Directory of Open Access Journals (Sweden)

María del Mar García-Calvente

2010-08-01

Full Text Available Objetivos: Analizar, con un enfoque de género, las percepciones y actitudes de los y las profesionales de atención primaria ante el cuidado informal. Métodos: Diseño cualitativo con entrevistas individuales y grupo de discusión. Mediante muestreo intencional se seleccionaron 18 profesionales de atención primaria (Distrito Sanitario de Granada. Se realizó un análisis de contenido con las categorías: a percepciones: conceptos de dependencia y cuidado informal, roles de género e impacto en salud; b actitudes: conservadora, actitud hacia el cambio y derecho a no cuidar. Resultados: Los profesionales y las destacaron el carácter profano, gratuito y con gran componente afectivo del cuidado informal; otorgaron a la familia, especialmente a las mujeres, la principal responsabilidad de cuidar, y recurrieron a estereotipos para diferenciar el cuidado dispensado por hombres y mujeres. Reconocieron que las mujeres presentan una mayor sobrecarga psicológica, principalmente por cuidar en solitario en mayor medida que los hombres. Emergieron tres actitudes ante el cuidado informal: ausencia de cuestionamiento de la situación actual e idealización de la familia como marco idóneo para cuidar; propuesta de cambio hacia una atención pública universal a la dependencia que descargue a las familias; y en una posición intermedia, actitud educadora para lograr el bienestar de las personas cuidadoras y evitar que dejen de cuidar. Conclusiones: Se identifican algunas percepciones y actitudes de profesionales ante el cuidado informal poco sensibles o potencialmente negativas para la equidad de género, como son la actitud conservadora que otorga a la familia la principal responsabilidad de cuidar y algunos estereotipos sexistas que atribuyen a las mujeres más capacidades para hacerlo. Se precisa formación específica de los y las profesionales para potenciar la incorporación del enfoque de género en el trabajo que realizan en relación con el cuidado

Assessment of diagnostics and antimicrobial susceptibility testing of Brachyspira species using a ring test

DEFF Research Database (Denmark)

Rasback, T.; Fellstrøm, C.; Bergsjo, B.

2005-01-01

used were Brachyspira hyodysenteriae, Brachyspira pilosicoli, Brachyspira innocens, Brachyspira murdochii and Brachyspira intermedia. In most cases, the correct Brachyspira spp. were detected. However, the results showed that Brachyspira spp. could be difficult to identify, especially if two...

Oral health and Brain Injury: Causal or Casual Relation?

DEFF Research Database (Denmark)

Pillai, Rajath; Iyer, Kiran; Spin-Neto, Rubens

2018-01-01

subtypes. Stroke prediction was associated with various factors such as number of teeth, periodontal conditions (even after controlling for confounding factors), clinical attachment loss, antibody levels to Aggregatibacter actinomycetemcomitans and Prevotella intermedia. The literature showed no consensus...

On two new species of Siriella (Mysidacea)

Digital Repository Service at National Institute of Oceanography (India)

Panampunnayil, S.U.

Descriptions of two new mysids, Siriella africana ap. nov. collected from Agulhas Bank and S. intermedia sp. nov. collected from Laccadives are given. The present paper contains observations on two species of mysidacea collected from the Indian...

Occurrence of periodontal pathogens in ethnic groups from a native Brazilian reservation.

Science.gov (United States)

Gaetti-Jardim, Elerson; Pereira, Maurício Fabiano; Vieira, Evanice Menezes Marçal; Schweitzer, Christiane Marie; Okamoto, Ana Cláudia; Ávila-Campos, Mario J

2015-06-01

The present study was designed to evaluate the occurrence of periodontal pathogens in the subgingival biofilm of 100 native Brazilians living at the Umutina Indian Reservation, Mato Grosso State, Brazil. Periodontal clinical examinations were carried out prior to collection of subgingival biofilm, and the presence of 14 periodontal microorganisms was evaluated by polymerase chain reaction (PCR). The prevalence and risk analysis was performed using Cochran and Mantel-Haenszel statistics for dichotomous variables or Pearson's chi-squared test for analysis of proportions when variables had three or more categories. The interrelations between clinical and microbiological parameters were assessed using Fisher's exact test and the Mann-Whitney U test. Individuals with chronic periodontitis were frequently colonized by the association between Porphyromonas gingivalis and Campylobacter rectus, P. gingivalis and Prevotella intermedia, or P. gingivalis and Tannerella forsythia. Patients with chronic periodontitis were also colonized by Porphyromonas gulae and P. intermedia or by the association between P. gulae and T. forsythia. P. gulae was detected only in the subgingival samples from natives on a traditional diet. Gingival bleeding was associated with Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, T. forsythia, P. gingivalis, P. gulae, Porphyromonas endodontalis, P. intermedia, and Prevotella nigrescens. Treponema denticola was uncommon. Peculiar microbiota was demonstrated to be associated with different periodontal disease statuses in native Brazilians, with modest occurrence of certain pathogens, such as T. denticola, and the presence of P. gulae in natives with gingivitis or chronic periodontitis. Copyright © 2015. Published by Elsevier Ltd.

Narativní scénáře a piktoriální modely

Czech Academy of Sciences Publication Activity Database

Fedrová, Stanislava; Jedličková, Alice

2 /19/, č. 2 (2010), s. 20-41 ISSN 1337-9275 Institutional research plan: CEZ:AV0Z90560517 Keywords : narrative * narrative script * inter-art relations * intermedia studies * pictorial model * ekphrasis Subject RIV: AJ - Letters, Mass-media, Audiovision

Association between periodontal condition and subgingival microbiota in women during pregnancy: a longitudinal study

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Priscila Viola BORGO

2014-12-01

Full Text Available Objectivo In this study, the gingival conditions and the quantitative detection for Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia in pregnant women were determined. Material and Methods Quantitative determinations of periodontal bacteria by using a SyBr green system in women during pregnancy were performed. Women at the 2nd and 3rd trimesters of pregnancy and non-pregnant women were included in this study. A. actinomycetemcomitans was observed in high numbers in women at the 2nd and 3rd trimesters of pregnancy with a significant difference (p<0.05. F. nucleatum and P. intermedia were also observed in high levels. Results and Conclusion Our results show that pregnant women are more susceptible to gingivitis, and the presence of A. actinomycetemcomitans in subgingival biofilm might be taken into account for the treatment of periodontal disease.

Technetium-99m radiolabeling of a recombinant dermonecrotic protein (recLiD1) from the Loxosceles venom for biodistribution study

International Nuclear Information System (INIS)

Valadares, D.; Felicori, L.; Olortegui, C.C.; Simal, C.; Gouvea dos Santos, R.

2007-01-01

In the present study the recombinant form (recLiD1) of a dermonecrotic protein present in the Brazilian brown spider Loxosceles intermedia venom was labeled with technetium-99m using stannous chloride and sodium borohydride as reducing agents. 99mTc-recLiD 1 kept its biological activity evoking dermonecrotic activity in rabbits. In vivo biodistribution in mice with the radiolabeled recLiD 1 showed high kidney uptake followed by stomach and liver uptakes. Also, we can see that 20% of toxin remaining in the skin after 120 min and once absorbed, 99mTc-recLiD 1 is rapidly cleared from the blood with long-lasting. We also observed one displacement of 99mTc-recLiD 1 by one monoclonal antibody raised against L. intermedia venom that indicates specific interaction with kidney tissue. (author)

Intermediários e as novas configurações no tecido organizacional brasileiro: Um estudo sobre as organizações sociais de saúde

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Julio Cesar Donadone

Full Text Available Resumo O artigo pretende discutir o surgimento e a configuração da intermediação, tomando como ponto de partida as transformações dos espaços organizacionais. Para tanto, utiliza como objeto de pesquisa a figura das organizações sociais de saúde, responsáveis por uma parte significativa dos hospitais estaduais em São Paulo. Enfatiza-se a reconfiguração do hospital causada pelas novas estratégias de gerenciamento provindas da intermediação que se estabelece, na saúde, entre o Estado e as organizações sociais. Assim, busca-se contribuir para a caracterização das formas de atuação e configuração dos intermediários no espaço social brasileiro.

O velikosti nosu, svědomí a metodách vyprávění: Weilova poetika po padesáti letech

Czech Academy of Sciences Publication Activity Database

Jedličková, Alice

2009-01-01

Roč. 60, č. 2 (2009), s. 68-75 ISSN 0009-0786 Institutional research plan: CEZ:AV0Z90560517 Keywords : Czech literature * Weil, Jiří * holocaust * Jews * narratology * intermedia studies Subject RIV: AJ - Letters, Mass-media, Audiovision

Direct 16S rRNA gene sequencing of polymicrobial culture-negative samples with analysis of mixed chromatograms

DEFF Research Database (Denmark)

Hartmeyer, Gitte N; Justesen, Ulrik S

2010-01-01

Two cases involving polymicrobial culture-negative samples were investigated by 16S rRNA gene sequencing, with analysis of mixed chromatograms. Fusobacterium necrophorum, Prevotella intermedia and Streptococcus constellatus were identified from pleural fluid in a patient with Lemierre's syndrome...

Biodiesel Fuel Quality and the ASTM Standard

Science.gov (United States)

Biodiesel is usually produced from vegetable oils, animal fats and used cooking oils with alternative feedstocks such as algae receiving increasing interest. The transesterification reaction which produces biodiesel also produces glycerol and proceeds stepwise via mono- and diacylglycerol intermedia...

Obraz v příběhu - ekfráze ve vyprávění. Předběžné návrhy k rozlišení popisnosti a vizuality

Czech Academy of Sciences Publication Activity Database

Jedličková, Alice; Fedrová, Stanislava

2010-01-01

Roč. 57, č. 1 (2010), s. 29-59 ISSN 0037-6973 Institutional research plan: CEZ:AV0Z90560517 Keywords : narrative * ekphrasis * visuality * descriptivity * intermedia studies * Urban, Miloš * Arbes, Jakub Subject RIV: AJ - Letters, Mass-media, Audiovision

Implante de lente intraocular trifocal difractivo: análisis y resultado de la agudeza visual

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Miguel Ángel Ibáñez-Hernández

2017-09-01

Conclusiones: El implante de un lente intraocular trifocal difractivo es capaz de proporcionar una excelente visión lejana, intermedia y cercana, con escasas aberraciones ópticas, permitiendo una mejor calidad visual útil en actividades desarrolladas a diferentes distancias.

Inform@ed space

DEFF Research Database (Denmark)

Bjerrum, Peter; Olsen, Kasper Nefer

2001-01-01

Inform@ed space Sensorial Perception And Computer Enchancement - bidrag til Nordisk Arkitekturforskningsforenings IT-konference, AAA april 2001.......Inform@ed space Sensorial Perception And Computer Enchancement - bidrag til Nordisk Arkitekturforskningsforenings IT-konference, AAA april 2001....

Microbial profile comparisons of saliva, pooled and site-specific subgingival samples in periodontitis patients

DEFF Research Database (Denmark)

Belstrøm, Daniel; Sembler-Møller, Maria Lynn; Grande, Maria Anastasia

2017-01-01

by pooled subgingival samples. Presence of Porphyromonas gingivalis, Treponema denticola, Prevotella intermedia, Filifactor alocis, Tannerella forsythia and Parvimona micra in site-specific subgingival samples were detected in saliva with an AUC of 0.79 (sensitivity: 0.61, specificity: 0.94), compared...

Workshop Polli Talu Loomingulises Keskuses

Index Scriptorium Estoniae

2004-01-01

MAP Intermedia Performance Collaboration'i (USA) workshop. Brendan McCall (liikumine), N. B. Aldrich (heli) ja Zach Poff (video) workshop tutvustab kolme kunstniku koostööd. Näidatakse ka multimeedia etendust, mis on külaliskunstnikel valminud Polli talus

The anti-adhesive mode of action of a purified mushroom (Lentinus edodes) extract with anticaries and antigingivitis properties in two oral bacterial phatogens

NARCIS (Netherlands)

Signoretto, C.; Marchi, A.; Bertoncelli, A.; Buralcchini, G.; Papetti, A.; Pruzzo, C.; Zaura, E.; Lingström, P.; Ofek, I.; Pratten, J.; Spratt, D.A.; Wilson, M.; Canepari, P.

2014-01-01

Background In previous works we have shown that a low-molecular-mass (LMM) fraction from mushroom (Lentinus edodes) homogenate interferes with binding of Streptococcus mutans to hydroxyapatite and Prevotella intermedia to gingival cells. Additionally, inhibition of biofilm formation of both odonto-

Ground beetle (Coleoptera: Carabidae) assemblages in narrow hedgerows in a Danish agricultural landscape

DEFF Research Database (Denmark)

Lövei, G. L.; Magura, T.

2015-01-01

Sorbus intermedia), and the non-native spruce (Picea spp.). We hypothesised that hedgerows with deciduous trees harbour more diverse ground beetle assemblages than hedges composed of non-native conifer trees. We also investigated which vegetation structure characteristics might influence the ground...