WorldWideScience

Sample records for single-particle multi-mode system

  1. New apparatus of single particle trap system for aerosol visualization

    Science.gov (United States)

    Higashi, Hidenori; Fujioka, Tomomi; Endo, Tetsuo; Kitayama, Chiho; Seto, Takafumi; Otani, Yoshio

    2014-08-01

    Control of transport and deposition of charged aerosol particles is important in various manufacturing processes. Aerosol visualization is an effective method to directly observe light scattering signal from laser-irradiated single aerosol particle trapped in a visualization cell. New single particle trap system triggered by light scattering pulse signal was developed in this study. The performance of the device was evaluated experimentally. Experimental setup consisted of an aerosol generator, a differential mobility analyzer (DMA), an optical particle counter (OPC) and the single particle trap system. Polystylene latex standard (PSL) particles (0.5, 1.0 and 2.0 μm) were generated and classified according to the charge by the DMA. Singly charged 0.5 and 1.0 μm particles and doubly charged 2.0 μm particles were used as test particles. The single particle trap system was composed of a light scattering signal detector and a visualization cell. When the particle passed through the detector, trigger signal with a given delay time sent to the solenoid valves upstream and downstream of the visualization cell for trapping the particle in the visualization cell. The motion of particle in the visualization cell was monitored by CCD camera and the gravitational settling velocity and the electrostatic migration velocity were measured from the video image. The aerodynamic diameter obtained from the settling velocity was in good agreement with Stokes diameter calculated from the electrostatic migration velocity for individual particles. It was also found that the aerodynamic diameter obtained from the settling velocity was a one-to-one function of the scattered light intensity of individual particles. The applicability of this system will be discussed.

  2. Exact solution for a time-dependent multi-mode coupled quadratic Bose system

    Energy Technology Data Exchange (ETDEWEB)

    Xu Xiuwei; Mu Haifeng [College of Physics, Ludong University, Yantai 264025 (China); Liu Shuyan [College of Electric and Electronic Engineering, Ludong University, Yantai 264025 (China); Guo Chun, E-mail: hai-fengmu@163.co [Office of Teaching Affairs, Ludong University, Yantai 264025 (China)

    2010-11-12

    By utilizing generalized linear quantum transformation theory, the evolution operator, normal and anti-normal Wigner characteristic functions, P- and Q-representations of a multi-mode coupled quadratic Boson system are presented. The squeezing properties of a time-dependent double-mode coupled quadratic Bose system are investigated as a specific example.

  3. Online probabilistic operational safety assessment of multi-mode engineering systems using Bayesian methods

    International Nuclear Information System (INIS)

    Lin, Yufei; Chen, Maoyin; Zhou, Donghua

    2013-01-01

    In the past decades, engineering systems become more and more complex, and generally work at different operational modes. Since incipient fault can lead to dangerous accidents, it is crucial to develop strategies for online operational safety assessment. However, the existing online assessment methods for multi-mode engineering systems commonly assume that samples are independent, which do not hold for practical cases. This paper proposes a probabilistic framework of online operational safety assessment of multi-mode engineering systems with sample dependency. To begin with, a Gaussian mixture model (GMM) is used to characterize multiple operating modes. Then, based on the definition of safety index (SI), the SI for one single mode is calculated. At last, the Bayesian method is presented to calculate the posterior probabilities belonging to each operating mode with sample dependency. The proposed assessment strategy is applied in two examples: one is the aircraft gas turbine, another is an industrial dryer. Both examples illustrate the efficiency of the proposed method

  4. A robo-pigeon based on an innovative multi-mode telestimulation system.

    Science.gov (United States)

    Yang, Junqing; Huai, Ruituo; Wang, Hui; Lv, Changzhi; Su, Xuecheng

    2015-01-01

    In this paper, we describe a new multi-mode telestimulation system for brain-microstimulation for the navigation of a robo-pigeon, a new type of bio-robot based on Brain-Computer Interface (BCI) techniques. The multi-mode telestimulation system overcomes neuron adaptation that was a key shortcoming of the previous single-mode stimulation by the use of non-steady TTL biphasic pulses accomplished by randomly alternating pulse modes. To improve efficiency, a new behavior model ("virtual fear") is proposed and applied to the robo-pigeon. Unlike the previous "virtual reward" model, the "virtual fear" behavior model does not require special training. The performance and effectiveness of the system to alleviate the adaptation of neurons was verified by a robo-pigeon navigation test, simultaneously confirming the practicality of the "virtual fear" behavioral model.

  5. Feasibility study to damp power system multi-mode oscillations by using a single FACTS device

    Energy Technology Data Exchange (ETDEWEB)

    Du, W.; Wu, X. [School of Electrical Engineering, Southeast University, Nanjing (China); Wang, H.F. [School of Electronics, Electrical Engineering and Computer Science, The Queen' s University of Belfast, Belfast BT7 1NN (United Kingdom); Dunn, R. [University of Bath, Bath (United Kingdom)

    2010-07-15

    To damp power system multi-mode oscillations, the commonly-used method is to arrange multiple decentralized stabilizers, such as PSS (Power System Stabilizer) and FACTS (Flexible AC Transmission Systems) stabilizers. In order to overcome the problem of interactions between stabilizers, coordinated design of multiple decentralized stabilizers has been proposed to simultaneously set parameters of all stabilizers. However, in practice it could be very difficult to implement the coordinated design of multiple stabilizers. This is because those stabilizers are often at different geographical locations in a power system and cross-location simultaneous field tuning of stabilizers' parameters is a tremendous task due to their interactions. Hence this paper proposes a novel scheme of damping power system multi-mode oscillations by using a single FACTS device and presents the results of feasibility study of the proposed scheme. It is demonstrated that multiple stabilizers can be arranged in a single FACTS device to effectively damp power system multi-mode oscillations. Under this scheme, multiple stabilizers are at a same geographical location in the power system and hence their parameters can be tuned simultaneously in coordination in the field. In the paper, three examples of multi-machine power systems installed with a UPFC (Unified Power Flow Controller), a STATCOM (Static Synchronous Compensator)/BESS (Battery Energy Storage System) and a MUPFC (Multiple-terminal UPFC) respectively are presented. Parameters of multiple stabilizers are designed in coordination by using a newly appeared method of optimisation-artificial fish swarm algorithm. Simulation results in the paper are compared with those obtained from applying the conventional scheme of decentralized control involving multiple PSSs. They demonstrate and confirm the feasibility of proposed scheme in the paper. (author)

  6. MIMO signal progressing with RLSCMA algorithm for multi-mode multi-core optical transmission system

    Science.gov (United States)

    Bi, Yuan; Liu, Bo; Zhang, Li-jia; Xin, Xiang-jun; Zhang, Qi; Wang, Yong-jun; Tian, Qing-hua; Tian, Feng; Mao, Ya-ya

    2018-01-01

    In the process of transmitting signals of multi-mode multi-core fiber, there will be mode coupling between modes. The mode dispersion will also occur because each mode has different transmission speed in the link. Mode coupling and mode dispersion will cause damage to the useful signal in the transmission link, so the receiver needs to deal received signal with digital signal processing, and compensate the damage in the link. We first analyzes the influence of mode coupling and mode dispersion in the process of transmitting signals of multi-mode multi-core fiber, then presents the relationship between the coupling coefficient and dispersion coefficient. Then we carry out adaptive signal processing with MIMO equalizers based on recursive least squares constant modulus algorithm (RLSCMA). The MIMO equalization algorithm offers adaptive equalization taps according to the degree of crosstalk in cores or modes, which eliminates the interference among different modes and cores in space division multiplexing(SDM) transmission system. The simulation results show that the distorted signals are restored efficiently with fast convergence speed.

  7. Integration of geospatial multi-mode transportation Systems in Kuala Lumpur

    International Nuclear Information System (INIS)

    Ismail, M A; Said, M N

    2014-01-01

    Public transportation serves people with mobility and accessibility to workplaces, health facilities, community resources, and recreational areas across the country. Development in the application of Geographical Information Systems (GIS) to transportation problems represents one of the most important areas of GIS-technology today. To show the importance of GIS network analysis, this paper highlights the determination of the optimal path between two or more destinations based on multi-mode concepts. The abstract connector is introduced in this research as an approach to integrate urban public transportation in Kuala Lumpur, Malaysia including facilities such as Light Rapid Transit (LRT), Keretapi Tanah Melayu (KTM) Komuter, Express Rail Link (ERL), KL Monorail, road driving as well as pedestrian modes into a single intelligent data model. To assist such analysis, ArcGIS's Network Analyst functions are used whereby the final output includes the total distance, total travelled time, directional maps produced to find the quickest, shortest paths, and closest facilities based on either time or distance impedance for multi-mode route analysis

  8. Integration of geospatial multi-mode transportation Systems in Kuala Lumpur

    Science.gov (United States)

    Ismail, M. A.; Said, M. N.

    2014-06-01

    Public transportation serves people with mobility and accessibility to workplaces, health facilities, community resources, and recreational areas across the country. Development in the application of Geographical Information Systems (GIS) to transportation problems represents one of the most important areas of GIS-technology today. To show the importance of GIS network analysis, this paper highlights the determination of the optimal path between two or more destinations based on multi-mode concepts. The abstract connector is introduced in this research as an approach to integrate urban public transportation in Kuala Lumpur, Malaysia including facilities such as Light Rapid Transit (LRT), Keretapi Tanah Melayu (KTM) Komuter, Express Rail Link (ERL), KL Monorail, road driving as well as pedestrian modes into a single intelligent data model. To assist such analysis, ArcGIS's Network Analyst functions are used whereby the final output includes the total distance, total travelled time, directional maps produced to find the quickest, shortest paths, and closest facilities based on either time or distance impedance for multi-mode route analysis.

  9. Role of single-particle and pair condensates in Bose systems with arbitrary intensity of interaction

    Directory of Open Access Journals (Sweden)

    A.S. Peletminskii

    2013-03-01

    Full Text Available We study a superfluid Bose system with single-particle and pair condensates on the basis of a half-phenomenological theory of a Bose liquid not involving the weakness of interparticle interaction. The coupled equations describing the equilibrium state of such system are derived from the variational principle for entropy. These equations are analyzed at zero temperature both analytically and numerically. It is shown that the fraction of particles in the single-particle and pair condensates essentially depends on the total density of the system. At densities attainable in condensates of alkali-metal atoms, almost all particles are in the single-particle condensate. The pair condensate fraction grows with increasing total density and becomes dominant. It is shown that at density of liquid helium, the single-particle condensate fraction is less than 10% that agrees with experimental data on inelastic neutron scattering, Monte Carlo calculations and other theoretical predictions. The ground state energy, pressure, and compressibility are found for the system under consideration. The spectrum of single-particle excitations is also analyzed.

  10. Label free imaging system for measuring blood flow speeds using a single multi-mode optical fiber (Conference Presentation)

    Science.gov (United States)

    Sigal, Iliya; Caravaca Aguirre, Antonio M.; Gad, Raanan; Piestun, Rafael; Levi, Ofer

    2016-03-01

    We demonstrate a single multi-mode fiber-based micro-endoscope for measuring blood flow speeds. We use the transmission-matrix wavefront shaping approach to calibrate the multi-mode fiber and raster-scan a focal spot across the distal fiber facet, imaging the cross-polarized back-reflected light at the proximal facet using a camera. This setup allows assessment of the backscattered photon statistics: by computing the mean speckle contrast values across the proximal fiber facet we show that spatially-resolved flow speed maps can be inferred by selecting an appropriate camera integration time. The proposed system is promising for minimally-invasive studies of neurovascular coupling in deep brain structures.

  11. Single-Particle Momentum Distributions of Efimov States in Mixed-Species Systems

    DEFF Research Database (Denmark)

    T. Yamashita, M.; F. Bellotti, F.; Frederico, T.

    2013-01-01

    to derive formulas for the scaling factor of the Efimov spectrum for any mass ratio assuming either that two or three of the two-body subsystems have a bound state at zero energy. We consider the single-particle momentum distribution analytically and numerically and analyse the tail of the momentum......We solve the three-body bound state problem in three dimensions for mass imbalanced systems of two identical bosons and a third particle in the universal limit where the interactions are assumed to be of zero-range. The system displays the Efimov effect and we use the momentum-space wave equation...... distribution to obtain the three-body contact parameter. Our finding demonstrate that the functional form of the three-body contact term depends on the mass ratio and we obtain an analytic expression for this behavior. To exemplify our results, we consider mixtures of Lithium with either two Caesium or Rubium...

  12. Use of anomolous thermal imaging effects for multi-mode systems control during crystal growth

    Science.gov (United States)

    Wargo, Michael J.

    1989-01-01

    Real time image processing techniques, combined with multitasking computational capabilities are used to establish thermal imaging as a multimode sensor for systems control during crystal growth. Whereas certain regions of the high temperature scene are presently unusable for quantitative determination of temperature, the anomalous information thus obtained is found to serve as a potentially low noise source of other important systems control output. Using this approach, the light emission/reflection characteristics of the crystal, meniscus and melt system are used to infer the crystal diameter and a linear regression algorithm is employed to determine the local diameter trend. This data is utilized as input for closed loop control of crystal shape. No performance penalty in thermal imaging speed is paid for this added functionality. Approach to secondary (diameter) sensor design and systems control structure is discussed. Preliminary experimental results are presented.

  13. TELAER: a multi-mode/multi-antenna interferometric airborne SAR system

    Science.gov (United States)

    Perna, Stefano; Amaral, Tiago; Berardino, Paolo; Esposito, Carmen; Jackson, Giuseppe; Pauciullo, Antonio; Vaz Junior, Eurico; Wimmer, Christian; Lanari, Riccardo

    2014-05-01

    The present contribution is aimed at showing the capabilities of the TELAER airborne Synthetic Aperture Radar (SAR) system recently upgraded to the interferometric mode [1]. TELAER is an Italian airborne X-Band SAR system, mounted onboard a LearJet 35A aircraft. Originally equipped with a single TX/RX antenna, it now operates in single-pass interferometric mode thanks to a system upgrading [1] funded by the Italian National Research Council (CNR), via the Italian Ministry of Education, Universities and Research (MIUR), in the framework of a cooperation between CNR and the Italian Agency for Agriculture Subsidy Payments (AGEA). In the frame of such cooperation, CNR has entrusted the Institute for Electromagnetic Sensing of the Environment (IREA) for managing all the activities, included the final flight tests, related to the system upgrading. According to such an upgrading, two additional receiving X-band antennas have been installed in order to allow, simultaneously, single-pass Across-Track and Along-Track interferometry [1]. More specifically, the three antennas are now installed in such a way to produce three different across-track baselines and two different along-track baselines. Moreover, in the frame of the same system upgrading, it has been mounted onboard the Learjet an accurate embedded Global Navigation Satellite System and Inertial Measurement Unit equipment. This allows precise measurement of the tracks described by the SAR antennas during the flight, in order to accurately implement Motion Compensation (MOCO) algorithms [2] during the image formation (focusing) step. It is worth remarking that the TELAER system upgraded to the interferometric mode is very flexible, since the user can set different operational modes characterized by different geometric resolutions and range swaths. In particular, it is possible to reach up to 0.5 m of resolution with a range swath of 2km; conversely, it is possible to enlarge the range swath up to 10 km at expenses of

  14. Multi-mode diagnosis of a gas turbine engine using an adaptive neuro-fuzzy system

    Directory of Open Access Journals (Sweden)

    Houman HANACHI

    2018-01-01

    Full Text Available Gas Turbine Engines (GTEs are vastly used for generation of mechanical power in a wide range of applications from airplane propulsion systems to stationary power plants. The gas-path components of a GTE are exposed to harsh operating and ambient conditions, leading to several degradation mechanisms. Because GTE components are mostly inaccessible for direct measurements and their degradation levels must be inferred from the measurements of accessible parameters, it is a challenge to acquire reliable information on the degradation conditions of the parts in different fault modes. In this work, a data-driven fault detection and degradation estimation scheme is developed for GTE diagnostics based on an Adaptive Neuro-Fuzzy Inference System (ANFIS. To verify the performance and accuracy of the developed diagnostic framework on GTE data, an ensemble of measurable gas path parameters has been generated by a high-fidelity GTE model under (a diverse ambient conditions and control settings, (b every possible combination of degradation symptoms, and (c a broad range of signal to noise ratios. The results prove the competency of the developed framework in fault diagnostics and reveal the sensitivity of diagnostic results to measurement noise for different degradation symptoms.

  15. A Research on Development of The Multi-mode Flood Forecasting System Version Management

    Science.gov (United States)

    Shen, J.-C.; Chang, C. H.; Lien, H. C.; Wu, S. J.; Horng, M. J.

    2009-04-01

    With the global economy and technological development, the degree of urbanization and population density relative to raise. At the same time, a natural buffer space and resources year after year, the situation has been weakened, not only lead to potential environmental disasters, more and more serious, disaster caused by the economy, loss of natural environment at all levels has been expanded. In view of this, the active participation of all countries in the world cross-sectoral integration of disaster prevention technology research and development, in addition, the specialized field of disaster prevention technology, science and technology development, network integration technology, high-speed data transmission and information to support the establishment of mechanisms for disaster management The decision-making and cross-border global disaster information network building and other related technologies, has become the international anti-disaster science and technology development trends, this trend. Naturally a few years in Taiwan, people's lives and property losses caused by many problems related to natural disaster prevention and disaster prevention and the establishment of applications has become a very important. For FEWS_Taiwan, flood warning system developed by the Delft Hydraulics and introduced the Water Resources Agency (WRA), it provides those functionalities for users to modify contents to add the basins, regions, data sources, models and etc. Despite this advantage, version differences due to different users or different teams yet bring about the difficulties on synchronization and integration.At the same time in different research teams will also add different modes of meteorological and hydrological data. From the government perspective of WRA, the need to plan standard operation procedures for system integration demands that the effort for version control due to version differences must be cost down or yet canceled out. As for FEWS_Taiwan, this

  16. Probing correlated quantum many-body systems at the single-particle level

    International Nuclear Information System (INIS)

    Endres, Manuel

    2013-01-01

    The detection of correlation and response functions plays a crucial role in the experimental characterization of quantum many-body systems. In this thesis, we present novel techniques for the measurement of such functions at the single-particle level. Specifically, we show the single-atom- and single-site-resolved detection of an ultracold quantum gas in an optical lattice. The quantum gas is described by the Bose-Hubbard model, which features a zero temperature phase transition from a superfluid to a Mott-insulating state, a paradigm example of a quantum phase transition. We used the aforementioned detection techniques to study correlation and response properties across the superfluid-Mott-insulator transition. The single-atom sensitivity of our method is achieved by fluorescence detection of individual atoms with a high signal-to-noise ratio. A high-resolution objective collects the fluorescence light and yields in situ 'snapshots' of the quantum gas that allow for a single-site-resolved reconstruction of the atomic distribution. This allowed us to measure two-site and non-local correlation-functions across the superfluid-Mott-insulator transition. Non-local correlation functions are based on the information of an extended region of the system and play an important role for the characterization of low-dimensional quantum phases. While non-local correlation functions were so far only theoretical tools, our results show that they are actually experimentally accessible. Furthermore, we used a new thermometry scheme, based on the counting of individual thermal excitations, to measure the response of the system to lattice modulation. Using this method, we studied the excitation spectrum of the system across the two-dimensional superfluid-Mott-insulator transition. In particular, we detected a 'Higgs' amplitude mode in the strongly-interacting superfluid close to the transition point where the system is described by an effectively Lorentz-invariant low-energy theory

  17. Probing correlated quantum many-body systems at the single-particle level

    Energy Technology Data Exchange (ETDEWEB)

    Endres, Manuel

    2013-02-27

    The detection of correlation and response functions plays a crucial role in the experimental characterization of quantum many-body systems. In this thesis, we present novel techniques for the measurement of such functions at the single-particle level. Specifically, we show the single-atom- and single-site-resolved detection of an ultracold quantum gas in an optical lattice. The quantum gas is described by the Bose-Hubbard model, which features a zero temperature phase transition from a superfluid to a Mott-insulating state, a paradigm example of a quantum phase transition. We used the aforementioned detection techniques to study correlation and response properties across the superfluid-Mott-insulator transition. The single-atom sensitivity of our method is achieved by fluorescence detection of individual atoms with a high signal-to-noise ratio. A high-resolution objective collects the fluorescence light and yields in situ 'snapshots' of the quantum gas that allow for a single-site-resolved reconstruction of the atomic distribution. This allowed us to measure two-site and non-local correlation-functions across the superfluid-Mott-insulator transition. Non-local correlation functions are based on the information of an extended region of the system and play an important role for the characterization of low-dimensional quantum phases. While non-local correlation functions were so far only theoretical tools, our results show that they are actually experimentally accessible. Furthermore, we used a new thermometry scheme, based on the counting of individual thermal excitations, to measure the response of the system to lattice modulation. Using this method, we studied the excitation spectrum of the system across the two-dimensional superfluid-Mott-insulator transition. In particular, we detected a 'Higgs' amplitude mode in the strongly-interacting superfluid close to the transition point where the system is described by an effectively Lorentz

  18. Single particle Green's functions calculation of the electrical conductivity of strong correlated systems

    International Nuclear Information System (INIS)

    Rodrigues Junior, W.A.

    A calculation of the electrical conductivity for Hubbard materials is presented which is valid when U/t >> 1 (U being the Coulomb repulsion and t the nearest neighbor hopping energy) for arbitrary electron concentration and temperature. The derivation emploies the single particle Green's functions with real and imaginary times instead of the usual two-particle real time Green's function. The result is compared with the experimental data available for some organic charge transfer salts [pt

  19. Continuous quantum mechanics of single particles in closed and quasi-closed systems: Pt. 1 and 2

    International Nuclear Information System (INIS)

    Brieger, M.

    2004-01-01

    The established statistical interpretation of quantum mechanics never envisioned our today's ability to handle and investigate single particles in trap devices. After scrutinizing the development of quantum mechanics, we point out that Schroedinger's equation establishes an energy representation, which obtains the energy eigenvalues as extrema of the energy curve or on the energy hypersurface, respectively. We also strongly emphasize its never exhausted capability of accounting in classical terms and full detail for the dynamics of single particles in closed systems. This is demonstrated for several familiar examples. They show that the eigensolutions to Schroedinger's equation must not blindly be identified with physically stationary states. The gained insight into the true dynamics allows to describe, without involving QED, the time evolution of a complete spontaneous transition as being driven by unbalanced internal dynamics. This mechanism relies on the fact that perfect balances are only possible in the exact extrema of the total energy and that any deviation, which is characterized by nonstationary states, makes multipole moments oscillate and emit electromagnetic radiation. (orig.)

  20. Continuous quantum mechanics of single particles in closed and quasi-closed systems: Pt. 1 and 2

    Energy Technology Data Exchange (ETDEWEB)

    Brieger, M. [DLR Deutsches Zentrum fuer Luft- und Raumfahrt e.V., Stuttgart (Germany). Inst. fuer Technische Physik

    2004-07-01

    The established statistical interpretation of quantum mechanics never envisioned our today's ability to handle and investigate single particles in trap devices. After scrutinizing the development of quantum mechanics, we point out that Schroedinger's equation establishes an energy representation, which obtains the energy eigenvalues as extrema of the energy curve or on the energy hypersurface, respectively. We also strongly emphasize its never exhausted capability of accounting in classical terms and full detail for the dynamics of single particles in closed systems. This is demonstrated for several familiar examples. They show that the eigensolutions to Schroedinger's equation must not blindly be identified with physically stationary states. The gained insight into the true dynamics allows to describe, without involving QED, the time evolution of a complete spontaneous transition as being driven by unbalanced internal dynamics. This mechanism relies on the fact that perfect balances are only possible in the exact extrema of the total energy and that any deviation, which is characterized by nonstationary states, makes multipole moments oscillate and emit electromagnetic radiation. (orig.)

  1. A single particle energies

    Energy Technology Data Exchange (ETDEWEB)

    Bodmer, A.R. [Illinois Univ., Chicago, IL (United States). Dept. of Physics]|[Argonne National Lab., IL (United States); Usmani, Q.N.; Sami, M. [Jamia Millia Islamia, New Delhi (India). Dept. of Physics

    1993-09-01

    We consider the binding energies of {Lambda} hypernuclei (HN), in particular the single-particle (s.p.) energy data, which have been obtained for a wide range of HN with mass numbers A {le} 89 and for orbital angular momenta {ell}{sub {Lambda}} {le} 4. We briefly review some of the relevant properties of A hypernuclei. These are nuclei {sub {Lambda}}{sup A}Z with baryon number A in which a single {Lambda} hyperon (baryon number = 1) is bound to an ordinary nucleus {sup A}Z consisting of A - 1 nucleons = Z protons + N neutrons. The {Lambda} hyperon is neutral, has spin 1/2, strangeness S = {minus}1, isospin I = O and a mass M{sub {Lambda}} = 1116 MeV/c{sup 2}. Although the {Lambda} interacts with a nucleon, its interaction is only about half as strong as that between two nucleons, and thus very roughly V{sub {Lambda}N} {approx} 0.5 V{sub NN}. As a result, the two-body {Lambda}N system is unbound, and the lightest bound HN is the three-body hypertriton {sub {Lambda}}{sup 3}H in which the {Lambda} is bound to a deuteron with the {Lambda}-d separation energy being only {approx} 0.1 MeV corresponding to an exponential tail of radius {approx} 15 fm! In strong interactions the strangeness S is of course conserved, and the {Lambda} is distinct from the nucleons. In a HN strangeness changes only in the weak decays of the {Lambda} which can decay either via ``free`` pionic decay {Lambda} {yields} N + {pi} or via induced decay {Lambda} + N {yields} N + N which is only possible in the presence of nucleons. Because of the small energy release the pionic decay is strongly suppressed in all but the lightest HN and the induced decay dominates. However, the weak decay lifetime {approx} 10{sup {minus}10}s is in fact close to the lifetime of a free {Lambda}. Since this is much longer than the strong interaction time {approx} 10{sup {minus}22}s we can ignore the weak interactions when considering the binding of HN, just as for ordinary nuclei.

  2. A single particle energies

    International Nuclear Information System (INIS)

    Bodmer, A.R.; Usmani, Q.N.; Sami, M.

    1993-01-01

    We consider the binding energies of Λ hypernuclei (HN), in particular the single-particle (s.p.) energy data, which have been obtained for a wide range of HN with mass numbers A ≤ 89 and for orbital angular momenta ell Λ ≤ 4. We briefly review some of the relevant properties of A hypernuclei. These are nuclei Λ A Z with baryon number A in which a single Λ hyperon (baryon number = 1) is bound to an ordinary nucleus A Z consisting of A - 1 nucleons = Z protons + N neutrons. The Λ hyperon is neutral, has spin 1/2, strangeness S = -1, isospin I = O and a mass M Λ = 1116 MeV/c 2 . Although the Λ interacts with a nucleon, its interaction is only about half as strong as that between two nucleons, and thus very roughly V ΛN ∼ 0.5 V NN . As a result, the two-body ΛN system is unbound, and the lightest bound HN is the three-body hypertriton Λ 3 H in which the Λ is bound to a deuteron with the Λ-d separation energy being only ∼ 0.1 MeV corresponding to an exponential tail of radius ∼ 15 fm exclamation point In strong interactions the strangeness S is of course conserved, and the Λ is distinct from the nucleons. In a HN strangeness changes only in the weak decays of the Λ which can decay either via ''free'' pionic decay Λ → N + π or via induced decay Λ + N → N + N which is only possible in the presence of nucleons. Because of the small energy release the pionic decay is strongly suppressed in all but the lightest HN and the induced decay dominates. However, the weak decay lifetime ∼ 10 -10 s is in fact close to the lifetime of a free Λ. Since this is much longer than the strong interaction time ∼ 10 -22 s we can ignore the weak interactions when considering the binding of HN, just as for ordinary nuclei

  3. Object recognition through a multi-mode fiber

    Science.gov (United States)

    Takagi, Ryosuke; Horisaki, Ryoichi; Tanida, Jun

    2017-04-01

    We present a method of recognizing an object through a multi-mode fiber. A number of speckle patterns transmitted through a multi-mode fiber are provided to a classifier based on machine learning. We experimentally demonstrated binary classification of face and non-face targets based on the method. The measurement process of the experimental setup was random and nonlinear because a multi-mode fiber is a typical strongly scattering medium and any reference light was not used in our setup. Comparisons between three supervised learning methods, support vector machine, adaptive boosting, and neural network, are also provided. All of those learning methods achieved high accuracy rates at about 90% for the classification. The approach presented here can realize a compact and smart optical sensor. It is practically useful for medical applications, such as endoscopy. Also our study indicated a promising utilization of artificial intelligence, which has rapidly progressed, for reducing optical and computational costs in optical sensing systems.

  4. Collective versus single-particle motion in quantum many-body systems from the perspective of an integrable model

    International Nuclear Information System (INIS)

    Haemmerling, Jens; Gutkin, Boris; Guhr, Thomas

    2010-01-01

    We study the emergence of collective dynamics in the integrable Hamiltonian system of two finite ensembles of coupled harmonic oscillators. After identification of a collective degree of freedom, the Hamiltonian is mapped onto a model of Caldeira-Leggett type, where the collective coordinate is coupled to an internal bath of phonons. In contrast to the usual Caldeira-Leggett model, the bath in the present case is part of the system. We derive an equation of motion for the collective coordinate which takes the form of a damped harmonic oscillator. We show that the distribution of quantum transition strengths induced by the collective mode is determined by its classical dynamics.

  5. Structure of the mycobacterial ESX-5 type VII secretion system membrane complex by single-particle analysis.

    Science.gov (United States)

    Beckham, Katherine S H; Ciccarelli, Luciano; Bunduc, Catalin M; Mertens, Haydyn D T; Ummels, Roy; Lugmayr, Wolfgang; Mayr, Julia; Rettel, Mandy; Savitski, Mikhail M; Svergun, Dmitri I; Bitter, Wilbert; Wilmanns, Matthias; Marlovits, Thomas C; Parret, Annabel H A; Houben, Edith N G

    2017-04-10

    Mycobacteria are characterized by their impermeable outer membrane, which is rich in mycolic acids 1 . To transport substrates across this complex cell envelope, mycobacteria rely on type VII (also known as ESX) secretion systems 2 . In Mycobacterium tuberculosis, these ESX systems are essential for growth and full virulence and therefore represent an attractive target for anti-tuberculosis drugs 3 . However, the molecular details underlying type VII secretion are largely unknown, due to a lack of structural information. Here, we report the molecular architecture of the ESX-5 membrane complex from Mycobacterium xenopi determined at 13 Å resolution by electron microscopy. The four core proteins of the ESX-5 complex (EccB 5 , EccC 5 , EccD 5 and EccE 5 ) assemble with equimolar stoichiometry into an oligomeric assembly that displays six-fold symmetry. This membrane-associated complex seems to be embedded exclusively in the inner membrane, which indicates that additional components are required to translocate substrates across the mycobacterial outer membrane. Furthermore, the extended cytosolic domains of the EccC ATPase, which interact with secretion effectors, are highly flexible, suggesting an as yet unseen mode of substrate interaction. Comparison of our results with known structures of other bacterial secretion systems demonstrates that the architecture of type VII secretion system is fundamentally different, suggesting an alternative secretion mechanism.

  6. Free-Propagator Reweighting Integrator for Single-Particle Dynamics in Reaction-Diffusion Models of Heterogeneous Protein-Protein Interaction Systems

    Directory of Open Access Journals (Sweden)

    Margaret E. Johnson

    2014-09-01

    Full Text Available We present a new algorithm for simulating reaction-diffusion equations at single-particle resolution. Our algorithm is designed to be both accurate and simple to implement, and to be applicable to large and heterogeneous systems, including those arising in systems biology applications. We combine the use of the exact Green’s function for a pair of reacting particles with the approximate free-diffusion propagator for position updates to particles. Trajectory reweighting in our free-propagator reweighting (FPR method recovers the exact association rates for a pair of interacting particles at all times. FPR simulations of many-body systems accurately reproduce the theoretically known dynamic behavior for a variety of different reaction types. FPR does not suffer from the loss of efficiency common to other path-reweighting schemes, first, because corrections apply only in the immediate vicinity of reacting particles and, second, because by construction the average weight factor equals one upon leaving this reaction zone. FPR applications include the modeling of pathways and networks of protein-driven processes where reaction rates can vary widely and thousands of proteins may participate in the formation of large assemblies. With a limited amount of bookkeeping necessary to ensure proper association rates for each reactant pair, FPR can account for changes to reaction rates or diffusion constants as a result of reaction events. Importantly, FPR can also be extended to physical descriptions of protein interactions with long-range forces, as we demonstrate here for Coulombic interactions.

  7. High transport efficiency of nanoparticles through a total-consumption sample introduction system and its beneficial application for particle size evaluation in single-particle ICP-MS.

    Science.gov (United States)

    Miyashita, Shin-Ichi; Mitsuhashi, Hiroaki; Fujii, Shin-Ichiro; Takatsu, Akiko; Inagaki, Kazumi; Fujimoto, Toshiyuki

    2017-02-01

    In order to facilitate reliable and efficient determination of both the particle number concentration (PNC) and the size of nanoparticles (NPs) by single-particle ICP-MS (spICP-MS) without the need to correct for the particle transport efficiency (TE, a possible source of bias in the results), a total-consumption sample introduction system consisting of a large-bore, high-performance concentric nebulizer and a small-volume on-axis cylinder chamber was utilized. Such a system potentially permits a particle TE of 100 %, meaning that there is no need to include a particle TE correction when calculating the PNC and the NP size. When the particle TE through the sample introduction system was evaluated by comparing the frequency of sharp transient signals from the NPs in a measured NP standard of precisely known PNC to the particle frequency for a measured NP suspension, the TE for platinum NPs with a nominal diameter of 70 nm was found to be very high (i.e., 93 %), and showed satisfactory repeatability (relative standard deviation of 1.0 % for four consecutive measurements). These results indicated that employing this total consumption system allows the particle TE correction to be ignored when calculating the PNC. When the particle size was determined using a solution-standard-based calibration approach without an NP standard, the particle diameters of platinum and silver NPs with nominal diameters of 30-100 nm were found to agree well with the particle diameters determined by transmission electron microscopy, regardless of whether a correction was performed for the particle TE. Thus, applying the proposed system enables NP size to be accurately evaluated using a solution-standard-based calibration approach without the need to correct for the particle TE.

  8. Polarization Characterization of a Multi-Moded Feed Structure

    Data.gov (United States)

    National Aeronautics and Space Administration — The Polarization Characterization of a Multi-Moded Feed Structure projects characterize the polarization response of a multi-moded feed horn as an innovative...

  9. Single Particle Entropy in Heated Nuclei

    International Nuclear Information System (INIS)

    Guttormsen, M.; Chankova, R.; Hjorth-Jensen, M.; Rekstad, J.; Siem, S.; Sunde, A. C.; Syed, N. U. H.; Agvaanluvsan, U.; Schiller, A.; Voinov, A.

    2006-01-01

    The thermal motion of single particles represents the largest contribution to level density (or entropy) in atomic nuclei. The concept of single particle entropy is presented and shown to be an approximate extensive (additive) quantity for mid-shell nuclei. A few applications of single particle entropy are demonstrated

  10. A theorem on the single particle energy in a Fermi gas with interaction

    NARCIS (Netherlands)

    Hugenholtz, N.M.; Hove, Léon van

    1958-01-01

    This paper investigates single particle properties in a Fermi gas with interaction at the absolute zero of temperature. In such a system a single particle energy has only a meaning for particles of momentum k close to the Fermi momentum kF. These single particle states are metastable with a

  11. Nanoscale three-dimensional single particle tracking.

    Science.gov (United States)

    Dupont, Aurélie; Lamb, Don C

    2011-11-01

    Single particle tracking (SPT) in biological systems is a quickly growing field. Many new technologies are being developed providing new tracking capabilities, which also lead to higher demands and expectations for SPT. Following a single biomolecule as it performs its function provides quantitative mechanistic information that cannot be obtained in classical ensemble methods. From the 3D trajectory, information is available over the diffusional behavior of the particle and precise position information can also be used to elucidate interactions of the tracked particle with its surroundings. Thus, three-dimensional (3D) SPT is a very valuable tool for investigating cellular processes. This review presents recent progress in 3D SPT, from image-based techniques toward more sophisticated feedback approaches. We focus mainly on the feedback technique known as orbital tracking. We present here a modified version of the original orbital tracking in which the intensities from two z-planes are simultaneously measured allowing a concomitant wide-field imaging. The system can track single particles with a precision down to 5 nm in the x-y plane and 7 nm in the axial direction. The capabilities of the system are demonstrated using single virus tracing to follow the infection pathway of Prototype Foamy Virus in living cells.

  12. Contribution to the development of a multi-mode measurement system for dynamic neutronic measurements and processing of the related uncertainties

    International Nuclear Information System (INIS)

    Geslot, B.

    2006-11-01

    It is difficult to estimate integral reactor parameters, especially reactivity, in deeply subcritical cores. Indeed the standard neutronic methods have been designed for near critical reactivity levels and they often need a critical reference. This thesis takes part in the research on ADS (Accelerated Driven Systems), for which the multiplication coefficient would be about 0.95. The first part of the thesis deals with the development of the XMODE system. It is a flexible measurement system dedicated to experiments in neutronics. X-MODE is capable of acquiring logical signals particularly in time-stamping mode as well as analogical signals. The second part of the thesis presents a statistical study of the methods used to analyse flux transients. Indeed a lot of methods exist to analyse flux transients and some are little known. Means to estimate characteristics of reactivity estimators are provided, methods compared and recommendations made. Finally, the dynamic measurements of the TRADE program are analysed and discussed. During this program, three subcritical configurations were explored. It appears that pulsed neutron source experiments give reactivity estimations that are much more precise than those obtained from flux transients. (author)

  13. Compressive multi-mode superresolution display

    KAUST Repository

    Heide, Felix

    2014-01-01

    Compressive displays are an emerging technology exploring the co-design of new optical device configurations and compressive computation. Previously, research has shown how to improve the dynamic range of displays and facilitate high-quality light field or glasses-free 3D image synthesis. In this paper, we introduce a new multi-mode compressive display architecture that supports switching between 3D and high dynamic range (HDR) modes as well as a new super-resolution mode. The proposed hardware consists of readily-available components and is driven by a novel splitting algorithm that computes the pixel states from a target high-resolution image. In effect, the display pixels present a compressed representation of the target image that is perceived as a single, high resolution image. © 2014 Optical Society of America.

  14. Development and Analysis of a Novel Multi-Mode MPPT Technique with Fast and Efficient Performance for PMSG-Based Wind Energy Conversion Systems

    Directory of Open Access Journals (Sweden)

    S. Heshmatian

    2018-03-01

    Full Text Available Wind energy is one of the most promising renewable energy resources. Due to instantaneous variations of the wind speed, an appropriate Maximum Power Point Tracking (MPPT method is necessary for maximizing the captured energy from the wind at different speeds. The most commonly used MPPT algorithms are Tip Speed Ratio (TSR, Power Signal Feedback (PSF, Optimal Torque Control (OTC and Hill Climbing Search (HCS. Each of these algorithms has some advantages and also some major drawbacks. In this paper, a novel hybrid MPPT algorithm is proposed which modifies the conventional methods in a way that eliminates their drawbacks and yields an improved performance. This proposed algorithm is faster in tracking the maximum power point and provides a more accurate response with lower steady state error. Moreover, it presents a great performance under conditions with intensive wind speed variations. The studied Wind Energy Conversion System (WECS consists of a Permanent Magnet Synchronous Generator (PMSG connected to the dc link through a Pulse-Width Modulated (PWM rectifier. The proposed algorithm and the conventional methods are applied to this WECS and their performances are compared using the simulation results. These results approve the satisfactory performance of the proposed algorithm and its notable advantages over the conventional methods.

  15. Integrated Method for Purification and Single-Particle Characterization of Lentiviral Vector Systems by Size Exclusion Chromatography and Tunable Resistive Pulse Sensing.

    Science.gov (United States)

    Heider, Susanne; Muzard, Julien; Zaruba, Marianne; Metzner, Christoph

    2017-07-01

    Elements derived from lentiviral particles such as viral vectors or virus-like particles are commonly used for biotechnological and biomedical applications, for example in mammalian protein expression, gene delivery or therapy, and vaccine development. Preparations of high purity are necessary in most cases, especially for clinical applications. For purification, a wide range of methods are available, from density gradient centrifugation to affinity chromatography. In this study we have employed size exclusion columns specifically designed for the easy purification of extracellular vesicles including exosomes. In addition to viral marker protein and total protein analysis, a well-established single-particle characterization technology, termed tunable resistive pulse sensing, was employed to analyze fractions of highest particle load and purity and characterize the preparations by size and surface charge/electrophoretic mobility. With this study, we propose an integrated platform combining size exclusion chromatography and tunable resistive pulse sensing for monitoring production and purification of viral particles.

  16. Multi-mode ultrasonic welding control and optimization

    Science.gov (United States)

    Tang, Jason C.H.; Cai, Wayne W

    2013-05-28

    A system and method for providing multi-mode control of an ultrasonic welding system. In one embodiment, the control modes include the energy of the weld, the time of the welding process and the compression displacement of the parts being welded during the welding process. The method includes providing thresholds for each of the modes, and terminating the welding process after the threshold for each mode has been reached, the threshold for more than one mode has been reached or the threshold for one of the modes has been reached. The welding control can be either open-loop or closed-loop, where the open-loop process provides the mode thresholds and once one or more of those thresholds is reached the welding process is terminated. The closed-loop control provides feedback of the weld energy and/or the compression displacement so that the weld power and/or weld pressure can be increased or decreased accordingly.

  17. Single-particle density matrix of liquid 4He

    International Nuclear Information System (INIS)

    Vakarchuk, I.A.

    2008-01-01

    The density single-particle matrix in the coordinate notation was calculated based on the expression for the interacting Bose-particle N system density matrix. Under the low temperatures the mentioned matrix in the first approximation enables to reproduce the Bogoliubov theory results. In the classical terms the mentioned theory enables to reproduce the results of the theory of the classical fluids in the approximation of the chaotic phases. On the basis of the density single-particle matrix one managed to obtain the function of the pulse distribution of the particles, the Bose-liquid average kinetic energy, and to study the Bose-Einstein condensation phenomenon [ru

  18. Single particle labeling of RNA virus in live cells.

    Science.gov (United States)

    Liu, Xiaohui; Ouyang, Ting; Ouyang, Hongsheng; Ren, Linzhu

    2017-06-02

    Real-time and visual tracking of viral infection is crucial for elucidating the infectious and pathogenesis mechanisms. To track the virus successfully, an efficient labeling method is necessary. In this review, we first discuss the practical labeling techniques for virus tracking in live cells. We then describe the current knowledge of interactions between RNA viruses (especially influenza viruses, immunodeficiency viruses, and Flaviviruses) and host cellular structures, obtained using single particle labeling techniques combined with real-time fluorescence microscopy. Single particle labeling provides an easy system for understanding the RNA virus life cycle. Copyright © 2017 Elsevier B.V. All rights reserved.

  19. Single particle degrees of freedom in the interacting boson model

    NARCIS (Netherlands)

    Scholten, O.

    1985-01-01

    An overview is given of different aspects of the Interacting Boson Fermion Model, the extension of the interacting Boson Model to odd mass nuclei. The microscopic model for the coupling of single-particle degrees of freedom to the system of bosons is outlined and the interaction between the bosons

  20. Squeezing in multi-mode nonlinear optical state truncation

    International Nuclear Information System (INIS)

    Said, R.S.; Wahiddin, M.R.B.; Umarov, B.A.

    2007-01-01

    In this Letter, we show that multi-mode qubit states produced via nonlinear optical state truncation driven by classical external pumpings exhibit squeezing condition. We restrict our discussions to the two- and three-mode cases

  1. Single particle raster image analysis of diffusion.

    Science.gov (United States)

    Longfils, M; Schuster, E; Lorén, N; Särkkä, A; Rudemo, M

    2017-04-01

    As a complement to the standard RICS method of analysing Raster Image Correlation Spectroscopy images with estimation of the image correlation function, we introduce the method SPRIA, Single Particle Raster Image Analysis. Here, we start by identifying individual particles and estimate the diffusion coefficient for each particle by a maximum likelihood method. Averaging over the particles gives a diffusion coefficient estimate for the whole image. In examples both with simulated and experimental data, we show that the new method gives accurate estimates. It also gives directly standard error estimates. The method should be possible to extend to study heterogeneous materials and systems of particles with varying diffusion coefficient, as demonstrated in a simple simulation example. A requirement for applying the SPRIA method is that the particle concentration is low enough so that we can identify the individual particles. We also describe a bootstrap method for estimating the standard error of standard RICS. © 2016 The Authors Journal of Microscopy © 2016 Royal Microscopical Society.

  2. Single-particle dispersion in compressible turbulence

    Science.gov (United States)

    Zhang, Qingqing; Xiao, Zuoli

    2018-04-01

    Single-particle dispersion statistics in compressible box turbulence are studied using direct numerical simulation. Focus is placed on the detailed discussion of effects of the particle Stokes number and turbulent Mach number, as well as the forcing type. When solenoidal forcing is adopted, it is found that the single-particle dispersion undergoes a transition from the ballistic regime at short times to the diffusive regime at long times, in agreement with Taylor's particle dispersion argument. The strongest dispersion of heavy particles is announced when the Stokes number is of order 1, which is similar to the scenario in incompressible turbulence. The dispersion tends to be suppressed as the Mach number increases. When hybrid solenoidal and compressive forcing at a ratio of 1/2 is employed, the flow field shows apparent anisotropic property, characterized by the appearance of large shock wave structures. Accordingly, the single-particle dispersion shows extremely different behavior from the solenoidal forcing case.

  3. Dual color single particle tracking via nanobodies

    International Nuclear Information System (INIS)

    Albrecht, David; Winterflood, Christian M; Ewers, Helge

    2015-01-01

    Single particle tracking is a powerful tool to investigate the function of biological molecules by following their motion in space. However, the simultaneous tracking of two different species of molecules is still difficult to realize without compromising the length or density of trajectories, the localization accuracy or the simplicity of the assay. Here, we demonstrate a simple dual color single particle tracking assay using small, bright, high-affinity labeling via nanobodies of accessible targets with widely available instrumentation. We furthermore apply a ratiometric step-size analysis method to visualize differences in apparent membrane viscosity. (paper)

  4. Projection operator treatment of single particle resonances

    International Nuclear Information System (INIS)

    Lev, A.; Beres, W.P.

    1976-01-01

    A projection operator method is used to obtain the energy and width of a single particle resonance. The resonance energy is found without scanning. An example of the first g/sub 9/2/ neutron resonance in 40 Ca is given and compared with the traditional phase shift method. The results of both approaches are quite similar. 4 figures

  5. The probe rules in single particle tracking

    DEFF Research Database (Denmark)

    Clausen, Mathias P.; Lagerholm, B. Christoffer

    2011-01-01

    Single particle tracking (SPT) enables light microscopy at a sub-diffraction limited spatial resolution by a combination of imaging at low molecular labeling densities and computational image processing. SPT and related single molecule imaging techniques have found a rapidly expanded use within...

  6. Real-time multi-mode neutron multiplicity counter

    Science.gov (United States)

    Rowland, Mark S; Alvarez, Raymond A

    2013-02-26

    Embodiments are directed to a digital data acquisition method that collects data regarding nuclear fission at high rates and performs real-time preprocessing of large volumes of data into directly useable forms for use in a system that performs non-destructive assaying of nuclear material and assemblies for mass and multiplication of special nuclear material (SNM). Pulses from a multi-detector array are fed in parallel to individual inputs that are tied to individual bits in a digital word. Data is collected by loading a word at the individual bit level in parallel, to reduce the latency associated with current shift-register systems. The word is read at regular intervals, all bits simultaneously, with no manipulation. The word is passed to a number of storage locations for subsequent processing, thereby removing the front-end problem of pulse pileup. The word is used simultaneously in several internal processing schemes that assemble the data in a number of more directly useable forms. The detector includes a multi-mode counter that executes a number of different count algorithms in parallel to determine different attributes of the count data.

  7. Single-particle Schroedinger fluid. I. Formulation

    International Nuclear Information System (INIS)

    Kan, K.K.; Griffin, J.J.

    1976-01-01

    The problem of a single quantal particle moving in a time-dependent external potential well is formulated specifically to emphasize and develop the fluid dynamical aspects of the matter flow. This idealized problem, the single-particle Schroedinger fluid, is shown to exhibit already a remarkably rich variety of fluid dynamical features, including compressible flow and line vortices. It provides also a sufficient framework to encompass simultaneously various simplified fluidic models for nuclei which have earlier been postulated on an ad hoc basis, and to illuminate their underlying restrictions. Explicit solutions of the single-particle Schroedinger fluid problem are studied in the adiabatic limit for their mathematical and physical implications (especially regarding the collective kinetic energy). The basic generalizations for extension of the treatment to the many-body Schroedinger fluid are set forth

  8. Single particle closed orbits in Yukawa potential

    Science.gov (United States)

    Mukherjee, R.; Sounda, S.

    2018-02-01

    Orbit of a single particle moving under the Yukawa potential is studied and there exists precessing ellipse type orbits. The amount of precession can be tuned through the coupling parameter α. With a suitable choice of the coupling parameter; we get a closed bound orbit. In some cases few petals are observed which is possessed of a closed bound nature for suitably chosen coupling parameter. Threshold energy has also been calculated for bound orbits.

  9. An efficient fluorescent single-particle position tracking system for long-term pulsed measurements of nitrogen-vacancy centers in diamond

    Science.gov (United States)

    Kim, Kiho; Yun, Jiwon; Lee, Donghyuck; Kim, Dohun

    2018-02-01

    A simple and convenient design enables real-time three-dimensional position tracking of nitrogen-vacancy (NV) centers in diamond. The system consists entirely of commercially available components (a single-photon counter, a high-speed digital-to-analog converter, a phase-sensitive detector-based feedback device, and a piezo stage), eliminating the need for custom programming or rigorous optimization processes. With a large input range of counters and trackers combined with high sensitivity of single-photon counting, high-speed position tracking (upper bound recovery time of 0.9 s upon 250 nm of step-like positional shift) not only of bright ensembles, but also of low-photon-collection-efficiency single to few NV centers (down to 103 s-1) is possible. The tracking requires position modulation of only 10 nm, which allows simultaneous position tracking and pulsed measurements in the long term. Therefore, this tracking system enables measuring a single-spin magnetic resonance and Rabi oscillations at a very high resolution even without photon collection optimization. The system is widely applicable to various fields related to NV center quantum manipulation research such as NV optical trapping, NV tracking in fluid dynamics, and biological sensing using NV centers inside a biological cell.

  10. Single Particle Tracking: Analysis Techniques for Live Cell Nanoscopy

    Science.gov (United States)

    Relich, Peter Kristopher, II

    Single molecule experiments are a set of experiments designed specifically to study the properties of individual molecules. It has only been in the last three decades where single molecule experiments have been applied to the life sciences; where they have been successfully implemented in systems biology for probing the behaviors of sub-cellular mechanisms. The advent and growth of super-resolution techniques in single molecule experiments has made the fundamental behaviors of light and the associated nano-probes a necessary concern amongst life scientists wishing to advance the state of human knowledge in biology. This dissertation disseminates some of the practices learned in experimental live cell microscopy. The topic of single particle tracking is addressed here in a format that is designed for the physicist who embarks upon single molecule studies. Specifically, the focus is on the necessary procedures to generate single particle tracking analysis techniques that can be implemented to answer biological questions. These analysis techniques range from designing and testing a particle tracking algorithm to inferring model parameters once an image has been processed. The intellectual contributions of the author include the techniques in diffusion estimation, localization filtering, and trajectory associations for tracking which will all be discussed in detail in later chapters. The author of this thesis has also contributed to the software development of automated gain calibration, live cell particle simulations, and various single particle tracking packages. Future work includes further evaluation of this laboratory's single particle tracking software, entropy based approaches towards hypothesis validations, and the uncertainty quantification of gain calibration.

  11. BIODEGRADATION AND DENTIN BONDING EFFECTIVENESS OF ONE "UNIVERSAL" SELF-ETCH ADHESIVE USED IN MULTI-MODE MANNER

    Directory of Open Access Journals (Sweden)

    Natalia Gateva

    2017-03-01

    Full Text Available A new type of one-step self-etch adhesives classified as "Universal" or "multi-mode" adhesives appeared in recent years. The idea is that these adhesives can be applied simultaneously with both techniques - etch and rinse and with self-etching technique, without compromising the bonding effectiveness. The aim of this study is to evaluate the micro-tensile adhesive bond strength to dentin of permanent teeth achieved after application of Single Bond Universal (3M ESPE adhesive system used in multi-mode manner. The results of our study show that the use of this universal adhesive system in multi-mode manner will not lead to the same results regarding the achieved bond strength with dentin. The additional etching with 37% phosphoric acid as well as the application of the adhesive in several layers deteriorates the bond strength right after application and after six months storage in artificial saliva medium (SAGF.

  12. Single-Particle States in $^{133}$Sn

    CERN Multimedia

    Huck, A

    2002-01-01

    % IS338 \\\\ \\\\ It is suggested to investigate the $\\beta^- $-decay of $^{133}$In and $^{134}$In in order to determine the single-particle states in $^{133}$Sn, which are so far unknown and needed for the shell-model description of the region close to $^{132}$Sn. Large hyper-pure Ge-detectors will be used for the $\\gamma$-ray spectroscopy. In the experiments with $^{134}$In, delayed neutrons in coincidence with $\\gamma$-rays from excited states in $^{133}$Sn provide the opportunity for a very selective detection of the states in question.

  13. Microorganism characterization by single particle mass spectrometry.

    Science.gov (United States)

    Russell, Scott C

    2009-01-01

    In recent years a major effort by several groups has been undertaken to identify bacteria by mass spectrometry at the single cell level. The intent of this review is to highlight the recent progress made in the application of single particle mass spectrometry to the analysis of microorganisms. A large portion of the review highlights improvements in the ionization and mass analysis of bio-aerosols, or particles that contain biologically relevant molecules such as peptides or proteins. While these are not direct applications to bacteria, the results have been central to a progression toward single cell mass spectrometry. Developments in single particle matrix-assisted laser desorption/ionization (MALDI) are summarized. Recent applications of aerosol laser desorption/ionization (LDI) to the analysis of single microorganisms are highlighted. Successful applications of off-line and on-the-fly aerosol MALDI to microorganism detection are discussed. Limitations to current approaches and necessary future achievements are also addressed. (c) 2009 Wiley Periodicals, Inc.

  14. Single particle tomography in EMAN2.

    Science.gov (United States)

    Galaz-Montoya, Jesús G; Flanagan, John; Schmid, Michael F; Ludtke, Steven J

    2015-06-01

    Single particle tomography (SPT or subtomogram averaging) offers a powerful alternative to traditional 2-D single particle reconstruction for studying conformationally or compositionally heterogeneous macromolecules. It can also provide direct observation (without labeling or staining) of complexes inside cells at nanometer resolution. The development of computational methods and tools for SPT remains an area of active research. Here we present the EMAN2.1 SPT toolbox, which offers a full SPT processing pipeline, from particle picking to post-alignment analysis of subtomogram averages, automating most steps. Different algorithm combinations can be applied at each step, providing versatility and allowing for procedural cross-testing and specimen-specific strategies. Alignment methods include all-vs-all, binary tree, iterative single-model refinement, multiple-model refinement, and self-symmetry alignment. An efficient angular search, Graphic Processing Unit (GPU) acceleration and both threaded and distributed parallelism are provided to speed up processing. Finally, automated simulations, per particle reconstruction of subtiltseries, and per-particle Contrast Transfer Function (CTF) correction have been implemented. Processing examples using both real and simulated data are shown for several structures. Copyright © 2015 Elsevier Inc. All rights reserved.

  15. Semi-quartic force fields retrieved from multi-mode expansions: Accuracy, scaling behavior, and approximations

    Energy Technology Data Exchange (ETDEWEB)

    Ramakrishnan, Raghunathan [Institute of Physical Chemistry and National Center for Computational Design and Discovery of Novel Materials (MARVEL), Department of Chemistry, University of Basel, Klingelbergstrasse 80, CH-4056 Basel (Switzerland); Rauhut, Guntram, E-mail: rauhut@theochem.uni-stuttgart.de [Institute for Theoretical Chemistry, University of Stuttgart, Pfaffenwaldring 55, D-70569 Stuttgart (Germany)

    2015-04-21

    Semi-quartic force fields (QFF) rely on a Taylor-expansion of the multi-dimensional Born-Oppenheimer potential energy surface (PES) and are frequently used within the calculation of anharmonic vibrational frequencies based on 2nd order vibrational perturbation theory (VPT2). As such they are usually determined by differentiation of the electronic energy with respect to the nuclear coordinates. Alternatively, potential energy surfaces can be expanded in terms of multi-mode expansions, which typically do not require any derivative techniques. The computational effort to retrieve QFF from size-reduced multi-mode expansions has been studied and has been compared with standard Taylor-expansions. As multi-mode expansions allow for the convenient introduction of subtle approximations, these will be discussed in some detail. In addition, a preliminary study about the applicability of a generalized Duschinsky transformation to QFFs is provided. This transformation allows for the efficient evaluation of VPT2 frequencies of isotopologues from the PES of the parent compound and thus avoids the recalculation of PESs in different axes systems.

  16. Semi-quartic force fields retrieved from multi-mode expansions: Accuracy, scaling behavior, and approximations

    International Nuclear Information System (INIS)

    Ramakrishnan, Raghunathan; Rauhut, Guntram

    2015-01-01

    Semi-quartic force fields (QFF) rely on a Taylor-expansion of the multi-dimensional Born-Oppenheimer potential energy surface (PES) and are frequently used within the calculation of anharmonic vibrational frequencies based on 2nd order vibrational perturbation theory (VPT2). As such they are usually determined by differentiation of the electronic energy with respect to the nuclear coordinates. Alternatively, potential energy surfaces can be expanded in terms of multi-mode expansions, which typically do not require any derivative techniques. The computational effort to retrieve QFF from size-reduced multi-mode expansions has been studied and has been compared with standard Taylor-expansions. As multi-mode expansions allow for the convenient introduction of subtle approximations, these will be discussed in some detail. In addition, a preliminary study about the applicability of a generalized Duschinsky transformation to QFFs is provided. This transformation allows for the efficient evaluation of VPT2 frequencies of isotopologues from the PES of the parent compound and thus avoids the recalculation of PESs in different axes systems

  17. Damping of unbound single-particle modes

    International Nuclear Information System (INIS)

    Fortier, S.; Beaumel, D.; Gales, S.; Guillot, J.; Langevin-Joliot, H.; Laurent, H.; Maison, J.M.; Bordewijk, J.; Brandenburg, S.; Krasznahorkay, A.; Crawley, G.M.; Massolo, C.P.; Renteria, M.; Khendriche, A.

    1995-01-01

    The (α, 3 He-n) reaction has been investigated at 120 MeV incident energy on 64 Ni, 90 Zr, and 120 Sn target nuclei. Neutrons in coincidence with 3 He particles emitted at 0 degree were detected using the multidetector array EDEN, in order to get information about the decay of single-particle states embedded in the (α, 3 He) continuum. Neutron angular correlations, multiplicity values, and branching ratios to low-lying states of the final nuclei have been compared with the predictions of the statistical decay model. Evidence for a significant nonstatistical decay branch has been observed in the three nuclei below about 15 MeV excitation energy. Direct branching ratios in 91 Zr deduced from this analysis are compared with the predictions of two nuclear structure models. At higher excitation energy, the decay characteristics of the (α, 3 He) continuum are shown to be mainly statistical

  18. Multiplex single particle analysis in microfluidics.

    Science.gov (United States)

    Dannhauser, D; Romeo, G; Causa, F; De Santo, I; Netti, P A

    2014-10-21

    A straightforward way to measure separated micrometric sized particles in microfluidic flow is reported. The light scattering profile (LSP) of each single particle is fully characterized by using a CMOS-camera based small angle light scattering (SALS) apparatus, ranging from 2° up to 30°. To ensure controlled particle passage through the incident laser, a viscoelastic 3D alignment effect by viscoelastic induced particle migration has been implemented in a simple and cost-effective microfluidic device. Different polystyrene particle sizes are measured in microfluidic flows and the obtained scattering signatures are matched with the Lorenz-Mie based scattering theory. The results confirm the possibility of using this apparatus for real multiplex particle analyses in microfluidic particle flows.

  19. Single particle tracking and single molecule energy transfer

    CERN Document Server

    Bräuchle, Christoph; Michaelis, Jens

    2009-01-01

    Closing a gap in the literature, this handbook gathers all the information on single particle tracking and single molecule energy transfer. It covers all aspects of this hot and modern topic, from detecting virus entry to membrane diffusion, and from protein folding using spFRET to coupled dye systems, as well recent achievements in the field. Throughout, the first-class editors and top international authors present content of the highest quality, making this a must-have for physical chemists, spectroscopists, molecular physicists and biochemists.

  20. Damping of unbound single-particle modes

    Energy Technology Data Exchange (ETDEWEB)

    Fortier, S.; Beaumel, D.; Gales, S.; Guillot, J.; Langevin-Joliot, H.; Laurent, H.; Maison, J.M.; Bordewijk, J.; Brandenburg, S.; Krasznahorkay, A.; Crawley, G.M.; Massolo, C.P.; Renteria, M.; Khendriche, A. [Institut de Physique Nucleaire, IN2P3-CNRS, 91406 Orsay Cedex (France)]|[Kernfysisch Versneller Instituut, 9747 AA Groningen (Netherlands)]|[Nuclear Research Institute, Debrecen P.O. Box 51, H-4001 (Hungary)]|[NSCL, Michigan State University, East Lansing, Michigan 48824 (United States)]|[Dep. Fisica, Fac. Cs. Exactas, UNLP, CC Nio 67, 1900 La Plata (Argentina)]|[Institut de Sciences Exactes,Universite de Tizi-Ouzou, 15000 Tizi-Ouzou (Algeria)

    1995-11-01

    The ({alpha},{sup 3}He-{ital n}) reaction has been investigated at 120 MeV incident energy on {sup 64}Ni, {sup 90}Zr, and {sup 120}Sn target nuclei. Neutrons in coincidence with {sup 3}He particles emitted at 0{degree} were detected using the multidetector array EDEN, in order to get information about the decay of single-particle states embedded in the ({alpha},{sup 3}He) continuum. Neutron angular correlations, multiplicity values, and branching ratios to low-lying states of the final nuclei have been compared with the predictions of the statistical decay model. Evidence for a significant nonstatistical decay branch has been observed in the three nuclei below about 15 MeV excitation energy. Direct branching ratios in {sup 91}Zr deduced from this analysis are compared with the predictions of two nuclear structure models. At higher excitation energy, the decay characteristics of the ({alpha},{sup 3}He) continuum are shown to be mainly statistical.

  1. Single particle level scheme for alpha decay

    International Nuclear Information System (INIS)

    Mirea, M.

    1998-01-01

    The fine structure phenomenon in alpha decay was evidenced by Rosenblum. In this process the kinetic energy of the emitted particle has several determined values related to the structure of the parent and the daughter nucleus. The probability to find the daughter in a low lying state was considered strongly dependent on the spectroscopic factor defined as the square of overlap between the wave function of the parent in the ground state and the wave functions of the specific excited states of the daughter. This treatment provides a qualitative agreement with the experimental results if the variations of the penetrability between different excited states are neglected. Based on single particle structure during fission, a new formalism explained quantitatively the fine structure of the cluster decay. It was suggested that this formalism can be applied also to alpha decay. For this purpose, the first step is to construct the level scheme of this type of decay. Such a scheme, obtained with the super-asymmetric two-center potential, is plotted for the alpha decay of 223 Ra. It is interesting to note that, diabatically, the level with spin 3/2 emerging from 1i 11/2 (ground state of the parent) reaches an excited state of the daughter in agreement with the experiment. (author)

  2. Brightness enhancement of a multi-mode ribbon fiber using transmitting Bragg gratings

    Science.gov (United States)

    Anderson, B. M.; Venus, G.; Ott, D.; Divliansky, I.; Dawson, J. W.; Drachenberg, D. R.; Messerly, M. J.; Pax, P. H.; Tassano, J. B.; Glebov, L. B.

    2015-03-01

    Increasing the dimensions of a waveguide provides the simplest means of reducing detrimental nonlinear effects, but such systems are inherently multi-mode, reducing the brightness of the system. Furthermore, using rectangular dimensions allows for improved heat extraction, as well as uniform temperature profile within the core. We propose a method of using the angular acceptance of a transmitting Bragg grating (TBG) to filter the fundamental mode of a fiber laser resonator, and as a means to increase the brightness of multi-mode fiber laser. Numerical modeling is used to calculate the diffraction losses needed to suppress the higher order modes in a laser system with saturable gain. The model is tested by constructing an external cavity resonator using an ytterbium doped ribbon fiber with core dimensions of 107.8μm by 8.3μm as the active medium. We show that the TBG increases the beam quality of the system from M2 = 11.3 to M2 = 1.45, while reducing the slope efficiency from 76% to 53%, overall increasing the brightness by 5.1 times.

  3. Microprocessor-based single particle calibration of scintillation counter

    Science.gov (United States)

    Mazumdar, G. K. D.; Pathak, K. M.

    1985-01-01

    A microprocessor-base set-up is fabricated and tested for the single particle calibration of the plastic scintillator. The single particle response of the scintillator is digitized by an A/D converter, and a 8085 A based microprocessor stores the pulse heights. The digitized information is printed. Facilities for CRT display and cassette storing and recalling are also made available.

  4. Automated data collection in single particle electron microscopy

    Science.gov (United States)

    Tan, Yong Zi; Cheng, Anchi; Potter, Clinton S.; Carragher, Bridget

    2016-01-01

    Automated data collection is an integral part of modern workflows in single particle electron microscopy (EM) research. This review surveys the software packages available for automated single particle EM data collection. The degree of automation at each stage of data collection is evaluated, and the capabilities of the software packages are described. Finally, future trends in automation are discussed. PMID:26671944

  5. Damage classification of pipelines under water flow operation using multi-mode actuated sensing technology

    International Nuclear Information System (INIS)

    Lee, Changgil; Park, Seunghee

    2011-01-01

    In a structure, several types of damage can occur, ranging from micro-cracking to corrosion or loose bolts. This makes identifying the damage difficult with a single mode of sensing. Therefore, a multi-mode actuated sensing system is proposed based on a self-sensing circuit using a piezoelectric sensor. In self-sensing-based multi-mode actuated sensing, one mode provides a wide frequency-band structural response from the self-sensed impedance measurement and the other mode provides a specific frequency-induced structural wavelet response from the self-sensed guided wave measurement. In this experimental study, a pipeline system under water flow operation was examined to verify the effectiveness and robustness of the proposed structural health monitoring approach. Different types of structural damage were inflicted artificially on the pipeline system. To classify the multiple types of structural damage, supervised learning-based statistical pattern recognition was implemented by composing a three-dimensional space using the damage indices extracted from the impedance and guided wave features as well as temperature variations. For a more systematic damage classification, several control parameters were optimized to determine an optimal decision boundary for the supervised learning-based pattern recognition. Further research issues are also discussed for real-world implementations of the proposed approach

  6. Multi-mode ultra-strong coupling in circuit quantum electrodynamics

    Science.gov (United States)

    Bosman, Sal J.; Gely, Mario F.; Singh, Vibhor; Bruno, Alessandro; Bothner, Daniel; Steele, Gary A.

    2017-10-01

    With the introduction of superconducting circuits into the field of quantum optics, many experimental demonstrations of the quantum physics of an artificial atom coupled to a single-mode light field have been realized. Engineering such quantum systems offers the opportunity to explore extreme regimes of light-matter interaction that are inaccessible with natural systems. For instance the coupling strength g can be increased until it is comparable with the atomic or mode frequency ωa,m and the atom can be coupled to multiple modes which has always challenged our understanding of light-matter interaction. Here, we experimentally realize a transmon qubit in the ultra-strong coupling regime, reaching coupling ratios of g/ωm = 0.19 and we measure multi-mode interactions through a hybridization of the qubit up to the fifth mode of the resonator. This is enabled by a qubit with 88% of its capacitance formed by a vacuum-gap capacitance with the center conductor of a coplanar waveguide resonator. In addition to potential applications in quantum information technologies due to its small size, this architecture offers the potential to further explore the regime of multi-mode ultra-strong coupling.

  7. A Multi-mode RCPSP with Stochastic Nonrenewable Resource Consumption

    DEFF Research Database (Denmark)

    Muller, Laurent Flindt

    , and thus improve bounds. In addition we experiment with cutting on the conic quadratic resource constraints. Computational experiments show that the branch-and-cut algorithm outperforms CPLEX 12.1. We finally examine the “cost of uncertainty” by investigating the relation between values of p, the makespan...... of estimations, and thus generally subject to uncertainty. If this uncertainty is not taken into account the resulting schedules may not be robust in the sense that, when executed, the uncertainty may cause the schedules to take longer than expected, consume more resources, or be outright infeasible. We propose...... a new variant of the Multi-mode Resource-Constrained Project Scheduling Problem, where the nonrenewable resource requirements of each mode is given by a Gaussian distribution, and the nonrenewable resource constraints must be satisfied with a certain probability p. Such constraints are also known...

  8. Single particle orbitals of the heaviest known actinide nuclei

    International Nuclear Information System (INIS)

    Ahmad, I.

    1992-01-01

    Single particle states in the actinide nuclei have been well characterized by decay scheme, (n, γ) and one nucleon transfer reaction studies. The energies of the single particle states are used to calculate the shell corrections which may give rise to stable superheavy elements. Large shell corrections for the superheavy elements arise from the gaps in the proton single-particle spectrum at Z = 114 and in the neutron single-particle spectrum at N = 184. The gap at Z = 114 is determined by the splitting of the f 7/2 and f 5/2 orbitals and the gap at N = 184 is determined by the locations of the h 11/2 , k 17/2 and j 13/2 spherical orbitals. Many of these states have been identified in very heavy actinide nuclei. Experiments identifying these states and the relation of the observed energies to the stability of superheavy elements are discussed

  9. Quantum chaos in nuclear single-particle motion and damping of giant resonances

    International Nuclear Information System (INIS)

    Pal, Santanu; Mukhopadhyay, Tapan

    1995-01-01

    The spectral statistics of single particle motion in deformed cavities with axial symmetry are presented. The single particle motion in the cavities considered are non-integrable and the systematics of the fluctuation measures of the spectra reveal a transition from regular to chaotic regime in the corresponding classical systems. Quantitative estimate of the degree of chaos enables us to introduce a correction factor to the one-body wall formula for the damping widths of isoscalar giant resonances. The damping widths calculated with this correction factor give much better agreement with experimental values than earlier calculations of one-body damping widths. (author). 21 refs., 5 figs

  10. Physics basis of Multi-Mode anomalous transport module

    Energy Technology Data Exchange (ETDEWEB)

    Rafiq, T.; Kritz, A. H.; Luo, L. [Department of Physics, Lehigh University, Bethlehem, Pennsylvania 18015 (United States); Weiland, J. [Departments of Applied Physics, Chalmers University of Technology and Euratom-VR Assoc., S41296 Gothenburg (Sweden); Pankin, A. Y. [Tech-X Corporation, Boulder, Colorado (United States)

    2013-03-15

    The derivation of Multi-Mode anomalous transport module version 8.1 (MMM8.1) is presented. The MMM8.1 module is advanced, relative to MMM7.1, by the inclusion of peeling modes, dependence of turbulence correlation length on flow shear, electromagnetic effects in the toroidal momentum diffusivity, and the option to compute poloidal momentum diffusivity. The MMM8.1 model includes a model for ion temperature gradient, trapped electron, kinetic ballooning, peeling, collisionless and collision dominated magnetohydrodynamics modes as well as model for electron temperature gradient modes, and a model for drift resistive inertial ballooning modes. In the derivation of the MMM8.1 module, effects of collisions, fast ion and impurity dilution, non-circular flux surfaces, finite beta, and Shafranov shift are included. The MMM8.1 is used to compute thermal, particle, toroidal, and poloidal angular momentum transports. The fluid approach which underlies the derivation of MMM8.1 is expected to reliably predict, on an energy transport time scale, the evolution of temperature, density, and momentum profiles in plasma discharges for a wide range of plasma conditions.

  11. Structure, single-particle and many-particle coefficients of Lennard ...

    Indian Academy of Sciences (India)

    We investigate the effects of temperature and density on the single-particle and many-particle coefficients as well as on the structures of homogenous systems in which the particles are assumed to interact via a continuous soft sphere potential in the microcanonical ensemble. The pair distribution function and therefore the ...

  12. Multi-mode excitation of a clamped–clamped microbeam resonator

    KAUST Repository

    Younis, Mohammad I.

    2015-02-18

    We present modeling and simulation of the nonlinear dynamics of a microresonator subjected to two-source electrostatic excitation. The resonator is composed of a clamped–clamped beam excited by a DC voltage load superimposed to two AC voltage loads of different frequencies. One frequency is tuned close to the first natural frequency of the beam and the other is close to the third (second symmetric) natural frequency. A multi-mode Galerkin procedure is applied to extract a reduced-order model, which forms the basis of the numerical simulations. Time history response, Poincare’ sections, Fast Fourier Transforms FFT, and bifurcation diagrams are used to reveal the dynamics of the system. The results indicate complex nonlinear phenomena, which include quasiperiodic motion, torus bifurcations, and modulated chaotic attractors.

  13. Brightness calibrates particle size in single particle fluorescence imaging.

    Science.gov (United States)

    Liu, Zhihe; Sun, Zezhou; Di, Weihua; Qin, Weiping; Yuan, Zhen; Wu, Changfeng

    2015-04-01

    This Letter provides a novel approach to quantify the particle sizes of highly bright semiconductor polymer dots (Pdots) for single-particle imaging and photobleaching studies. A quadratic dependence of single-particle brightness on particle size was determined by single-particle fluorescence imaging and intensity statistics. In terms of the same imaging conditions, the particle diameter can be quantified by comparing the individual brightness intensity with associated calibration curve. Based on this sizing method, photobleaching trajectories and overall photon counts emitted by single particles were analyzed. It is found that photobleaching rate constants of different sized Pdots are not strongly dependent on particle diameter except the sparsely occurring fluorescence blinking in certain dim particles and the rapid photobleaching component in some bright particles. The overall photon counts increase with increasing particle diameter. However, those larger than 30 nm deviate away from the increasing tendency. These results reveal the significance of selecting appropriate Pdots (≤30  nm) for single-particle imaging and tracking applications.

  14. Evolution of single-particle structure of silicon isotopes

    Science.gov (United States)

    Bespalova, O. V.; Fedorov, N. A.; Klimochkina, A. A.; Markova, M. L.; Spasskaya, T. I.; Tretyakova, T. Yu.

    2018-01-01

    New data on proton and neutron single-particle energies E_{nlj} of Si isotopes with neutron number N from 12 to 28 as well as occupation probabilities N_{nlj} of single-particle states of stable isotopes 28, 30Si near the Fermi energy were obtained by the joint evaluation of the stripping and pick-up reaction data and excited state decay schemes of neighboring nuclei. The evaluated data indicate the following features of single-particle structure evolution: persistence of Z = 14 subshell closure with N increase, the new magicity of the number N = 16, and the conservation of the magic properties of the number N = 20 in Si isotopic chain. The features were described by the dispersive optical model. The calculation also predicts the weakening of N = 28 shell closure and demonstrates evolution of a bubble-like structure of the proton density distributions in neutron-rich Si isotopes.

  15. High Fidelity Multi-Mode Hyperspectral Multispectral Imager with Programmable Spectral Resolution, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — This SBIR Phase II proposal introduces a fast multi-mode hyperspectral-multispectral (MM-HS-MS) sensor with programmable spectral resolution. The sensor brings the...

  16. Decay properties of high-lying single-particles modes

    NARCIS (Netherlands)

    Beaumel, D; Fortier, S; Gales, S; Guillot, J; LangevinJoliot, H; Laurent, H; Maison, JM; Vernotte, J; Bordewijck, J; Brandenburg, S; Krasznahorkay, A; Crawley, GM; Massolo, CP; Renteria, M; Khendriche, A

    1996-01-01

    The neutron decay of high-lying single-particle states in Ni-64, Zr-90, Sn-120 and (208)pb excited by means of the (alpha,He-3) reaction has been investigated at 120 MeV incident energy using the multidetector EDEN. The characteristics of this reaction are studied using inclusive spectra and angular

  17. Single-particle spectral density of the Hubbard model

    NARCIS (Netherlands)

    Mehlig, B.; Eskes, H.; Hayn, R.; Meinders, M.B.J.

    1995-01-01

    We calculate the single-particle spectral function for the Hubbard model within the framework of a projection technique equivalent to the two-pole approximation. We show that the two-pole approximation can be well understood as an average characterization of the upper and the lower Hubbard bands,

  18. SINGLE-PARTICLE SPECTRAL DENSITY OF THE HUBBARD-MODEL

    NARCIS (Netherlands)

    MEHLIG, B; ESKES, H; HAYN, R; MEINDERS, MBJ

    1995-01-01

    We calculate the single-particle spectral function for the Hubbard model within the framework of a projection technique equivalent to the two-pole approximation. We show that the two-pole approximation can be well understood as an average characterization of the upper and the lower Hubbard bands,

  19. Ergodicity of a single particle confined in a nanopore

    DEFF Research Database (Denmark)

    Bernardi, S.; Hansen, Jesper Schmidt; Frascolli, F.

    2012-01-01

    -ergodic component of the phase space for energy levels typical of experiments, is surprisingly small, i.e. we conclude that the ergodic hypothesis is a reasonable approximation even for a single particle trapped in a nanopore. Due to the numerical scope of this work, our focus will be the onset of ergodic behavior...

  20. Calibration of single particle sizing velocimeters using photomask reticles

    Science.gov (United States)

    Hirleman, E. D.; Holve, D. J.; Hovenac, E. A.

    1988-01-01

    The development of photomask reticle calibration standards for single particle instruments is discussed. The calibration method studied involves the use of photomask reticles where the particle artifacts are actually disks of chrome thin film in the clear field reticles produced by photolithography and etching processes. Consideration is given to various aspects of theory, design, and performance.

  1. Influence of Torrefaction on Single Particle Combustion of Wood

    DEFF Research Database (Denmark)

    Lu, Zhimin; Jian, Jie; Jensen, Peter Arendt

    2016-01-01

    This study focuses on the influence of torrefaction on the char reactivity, char yield, and combustion time of 3-5 mm spherical wood particles in a single particle combustion reactor (SPC) operating at a nominal temperature of 1231 °C. The devolatilization times were reduced and the char burnout...

  2. Efficient multi-mode to single-mode conversion in a 61 port photonic lantern

    DEFF Research Database (Denmark)

    Noordegraaf, Danny; Skovgaard, Peter M. W.; Dybendahl Maack, Martin

    2010-01-01

    We demonstrate the fabrication of a multi-mode (MM) to 61 port single-mode (SM) splitter or "Photonic Lantern". Low port count Photonic Lanterns were first described by Leon-Saval et al. (2005). These are based on a photonic crystal fiber type design, with air-holes defining the multi-mode fiber ...... of astrophotonics for coupling MM star-light to an ensemble of SM fibers in order to perform fiber Bragg grating based spectral filtering....

  3. Single-particle response function in finite nuclei

    International Nuclear Information System (INIS)

    Shlomo, S.; Texas A and M Univ., College Station

    1982-01-01

    I derive expressions for the single-particle response (structure) function S(E, q) and its sum rule, (Pauli blocking factor) P(q) = ∫ dE S(E, q), in terms of the Wiqner transforms (WTs) of the single-particle wave functions and the scattering probe sigma(q, r) and discuss the semi-classical phase-space interpretation of the results. For sigma(q, r) = esup(iq x r), I derive simple expressions for S(E, q) and P(q) for finite nuclei within the harmonic-oscillator model and compare the results with the well-known results of the Fermi-gas model. (orig.)

  4. Particle segmentation algorithm for flexible single particle reconstruction.

    Science.gov (United States)

    Zhou, Qiang; Zhou, Niyun; Wang, Hong-Wei

    2017-01-01

    As single particle cryo-electron microscopy has evolved to a new era of atomic resolution, sample heterogeneity still imposes a major limit to the resolution of many macromolecular complexes, especially those with continuous conformational flexibility. Here, we describe a particle segmentation algorithm towards solving structures of molecules composed of several parts that are relatively flexible with each other. In this algorithm, the different parts of a target molecule are segmented from raw images according to their alignment information obtained from a preliminary 3D reconstruction and are subjected to single particle processing in an iterative manner. This algorithm was tested on both simulated and experimental data and showed improvement of 3D reconstruction resolution of each segmented part of the molecule than that of the entire molecule.

  5. Single-particle cryo-electron microscopy of macromolecular complexes.

    Science.gov (United States)

    Skiniotis, Georgios; Southworth, Daniel R

    2016-02-01

    Recent technological breakthroughs in image acquisition have enabled single-particle cryo-electron microscopy (cryo-EM) to achieve near-atomic resolution structural information for biological complexes. The improvements in image quality coupled with powerful computational methods for sorting distinct particle populations now also allow the determination of compositional and conformational ensembles, thereby providing key insights into macromolecular function. However, the inherent instability and dynamic nature of biological assemblies remain a tremendous challenge that often requires tailored approaches for successful implementation of the methodology. Here, we briefly describe the fundamentals of single-particle cryo-EM with an emphasis on covering the breadth of techniques and approaches, including low- and high-resolution methods, aiming to illustrate specific steps that are crucial for obtaining structural information by this method. © The Author 2015. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  6. Single particle electrochemical sensors and methods of utilization

    Science.gov (United States)

    Schoeniger, Joseph [Oakland, CA; Flounders, Albert W [Berkeley, CA; Hughes, Robert C [Albuquerque, NM; Ricco, Antonio J [Los Gatos, CA; Wally, Karl [Lafayette, CA; Kravitz, Stanley H [Placitas, NM; Janek, Richard P [Oakland, CA

    2006-04-04

    The present invention discloses an electrochemical device for detecting single particles, and methods for using such a device to achieve high sensitivity for detecting particles such as bacteria, viruses, aggregates, immuno-complexes, molecules, or ionic species. The device provides for affinity-based electrochemical detection of particles with single-particle sensitivity. The disclosed device and methods are based on microelectrodes with surface-attached, affinity ligands (e.g., antibodies, combinatorial peptides, glycolipids) that bind selectively to some target particle species. The electrodes electrolyze chemical species present in the particle-containing solution, and particle interaction with a sensor element modulates its electrolytic activity. The devices may be used individually, employed as sensors, used in arrays for a single specific type of particle or for a range of particle types, or configured into arrays of sensors having both these attributes.

  7. Single Particle Orientation and Rotational Tracking (SPORT) in biophysical studies

    Science.gov (United States)

    Gu, Yan; Ha, Ji Won; Augspurger, Ashley E.; Chen, Kuangcai; Zhu, Shaobin; Fang, Ning

    2013-10-01

    The single particle orientation and rotational tracking (SPORT) techniques have seen rapid development in the past 5 years. Recent technical advances have greatly expanded the applicability of SPORT in biophysical studies. In this feature article, we survey the current development of SPORT and discuss its potential applications in biophysics, including cellular membrane processes and intracellular transport.The single particle orientation and rotational tracking (SPORT) techniques have seen rapid development in the past 5 years. Recent technical advances have greatly expanded the applicability of SPORT in biophysical studies. In this feature article, we survey the current development of SPORT and discuss its potential applications in biophysics, including cellular membrane processes and intracellular transport. Electronic supplementary information (ESI) available: Three supplementary movies and an experimental section. See DOI: 10.1039/c3nr02254d

  8. Spin resonance strength calculation through single particle tracking for RHIC

    Energy Technology Data Exchange (ETDEWEB)

    Luo, Y. [Brookhaven National Lab. (BNL), Upton, NY (United States); Dutheil, Y. [Brookhaven National Lab. (BNL), Upton, NY (United States); Huang, H. [Brookhaven National Lab. (BNL), Upton, NY (United States); Meot, F. [Brookhaven National Lab. (BNL), Upton, NY (United States); Ranjbar, V. [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2015-05-03

    The strengths of spin resonances for the polarized-proton operation in the Relativistic Heavy Ion Collider are currently calculated with the code DEPOL, which numerically integrates through the ring based on an analytical approximate formula. In this article, we test a new way to calculate the spin resonance strengths by performing Fourier transformation to the actual transverse magnetic fields seen by a single particle traveling through the ring. Comparison of calculated spin resonance strengths is made between this method and DEPOL.

  9. Spatially Resolved Sensitivity of Single-Particle Plasmon Sensors.

    Science.gov (United States)

    Beuwer, Michael A; van Hoof, Bas; Zijlstra, Peter

    2018-03-01

    The high sensitivity of localized surface plasmon resonance sensors to the local refractive index allows for the detection of single-molecule binding events. Though binding events of single objects can be detected by their induced plasmon shift, the broad distribution of observed shifts remains poorly understood. Here, we perform a single-particle study wherein single nanospheres bind to a gold nanorod, and relate the observed plasmon shift to the binding location using correlative microscopy. To achieve this we combine atomic force microscopy to determine the binding location, and single-particle spectroscopy to determine the corresponding plasmon shift. As expected, we find a larger plasmon shift for nanospheres binding at the tip of a rod compared to its sides, in good agreement with numerical calculations. However, we also find a broad distribution of shifts even for spheres that were bound at a similar location to the nanorod. Our correlative approach allows us to disentangle effects of nanoparticle dimensions and binding location, and by comparison to numerical calculations we find that the biggest contributor to this observed spread is the dispersion in nanosphere diameter. These experiments provide insight into the spatial sensitivity and signal-heterogeneity of single-particle plasmon sensors and provides a framework for signal interpretation in sensing applications.

  10. Single Particle Nanoplasmonic Sensing in Individual Nanofluidic Channels.

    Science.gov (United States)

    Fritzsche, Joachim; Albinsson, David; Fritzsche, Michael; Antosiewicz, Tomasz J; Westerlund, Fredrik; Langhammer, Christoph

    2016-12-14

    Nanoplasmonics allows label-free optical sensing and spectroscopy at the single nanoparticle level by exploiting plasmonic excitations in metal nanoparticles. Nanofluidics offers exclusive possibilities for applying and controlling fluid flow and mass transport at the nanoscale and toward nanosized objects. Here, we combine these two concepts in a single device, by integrating single particle nanoplasmonic sensing with nanofluidics using advanced nanofabrication. The developed devices enable on-chip referenced parallel single particle nanoplasmonic sensing inside multiple individual nanofluidic channels with dimensions down to the 100 nm range. Beyond detailed discussion of the nanofabrication, general device characterization, and parallelized single particle plasmonic readout concepts, we demonstrate device function on two examples: (i) in situ measurements of local buffer concentrations inside a nanofluidic channel; (ii) real time binding kinetics of alkanethiol molecules to a single plasmonic nanonatenna sensor in a single nanochannel. Our concept thus provides a powerful solution for controlling mass transport to and from individual (plasmonic) nanoparticles, which in a long-term perspective offers unique opportunities for label-free detection of analyte molecules at low concentrations and for fundamental studies of fluids in extreme confinement.

  11. Inequivalence of single-particle and population lifetimes in a cuprate superconductor

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Shuolong [SLAC National Accelerator Lab., Menlo Park, CA (United States); Stanford Univ., Stanford, CA (United States); Sobota, J. A. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Leuenberger, D. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Stanford Univ., Stanford, CA (United States); He, Y. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Stanford Univ., Stanford, CA (United States); Hashimoto, M. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Lu, D. H. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Eisaki, H. [National Institute of Advanced Industrial Science and Technology, Ibaraki (Japan); Kirchmann, P. S. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Shen, Z. -X. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Stanford Univ., Stanford, CA (United States)

    2015-06-15

    We study optimally doped Bi-2212 (Tc=96 K) using femtosecond time- and angle-resolved photoelectron spectroscopy. Energy-resolved population lifetimes are extracted and compared with single-particle lifetimes measured by equilibrium photoemission. The population lifetimes deviate from the single-particle lifetimes in the low excitation limit by 1–2 orders of magnitude. Fundamental considerations of electron scattering unveil that these two lifetimes are in general distinct, yet for systems with only electron-phonon scattering they should converge in the low-temperature, low-fluence limit. As a result, the qualitative disparity in our data, even in this limit, suggests that scattering channels beyond electron-phonon interactions play a significant role in the electron dynamics of cuprate superconductors.

  12. Guidelines for the fitting of anomalous diffusion mean square displacement graphs from single particle tracking experiments.

    Directory of Open Access Journals (Sweden)

    Eldad Kepten

    Full Text Available Single particle tracking is an essential tool in the study of complex systems and biophysics and it is commonly analyzed by the time-averaged mean square displacement (MSD of the diffusive trajectories. However, past work has shown that MSDs are susceptible to significant errors and biases, preventing the comparison and assessment of experimental studies. Here, we attempt to extract practical guidelines for the estimation of anomalous time averaged MSDs through the simulation of multiple scenarios with fractional Brownian motion as a representative of a large class of fractional ergodic processes. We extract the precision and accuracy of the fitted MSD for various anomalous exponents and measurement errors with respect to measurement length and maximum time lags. Based on the calculated precision maps, we present guidelines to improve accuracy in single particle studies. Importantly, we find that in some experimental conditions, the time averaged MSD should not be used as an estimator.

  13. Digital atom interferometer with single particle control on a discretized space-time geometry.

    Science.gov (United States)

    Steffen, Andreas; Alberti, Andrea; Alt, Wolfgang; Belmechri, Noomen; Hild, Sebastian; Karski, Michał; Widera, Artur; Meschede, Dieter

    2012-06-19

    Engineering quantum particle systems, such as quantum simulators and quantum cellular automata, relies on full coherent control of quantum paths at the single particle level. Here we present an atom interferometer operating with single trapped atoms, where single particle wave packets are controlled through spin-dependent potentials. The interferometer is constructed from a sequence of discrete operations based on a set of elementary building blocks, which permit composing arbitrary interferometer geometries in a digital manner. We use this modularity to devise a space-time analogue of the well-known spin echo technique, yielding insight into decoherence mechanisms. We also demonstrate mesoscopic delocalization of single atoms with a separation-to-localization ratio exceeding 500; this result suggests their utilization beyond quantum logic applications as nano-resolution quantum probes in precision measurements, being able to measure potential gradients with precision 5 x 10(-4) in units of gravitational acceleration g.

  14. Three-dimensional single particle tracking in dense dust clouds by stereoscopy of fluorescent particles

    Science.gov (United States)

    Himpel, Michael; Killer, Carsten; Buttenschön, Birger; Melzer, André

    2012-12-01

    In dense dust clouds of a dusty plasma single particle trajectories are impossible to follow due to occlusion of particles and ambiguities in particle correspondences. By stereoscopic imaging of fluorescent tracer particles, we were able to reconstruct 3D single particle trajectories within dense dust clouds. Several measurements are shown that justify to regard the tracer particles as suitable representatives for the whole dust system. A first analysis of dust density waves in dense clouds already shows that these waves exhibit three-dimensional dynamics at larger wave amplitudes that cannot be resolved by 2D imaging techniques: a broad velocity distribution perpendicular to the oscillation plane due to dust-dust collisions is seen, while the velocity distribution in the oscillation direction is bimodal and shifted due to the bulk wave propagation.

  15. Three-dimensional single particle tracking in dense dust clouds by stereoscopy of fluorescent particles

    Energy Technology Data Exchange (ETDEWEB)

    Himpel, Michael; Killer, Carsten; Buttenschoen, Birger; Melzer, Andre [Ernst-Moritz-Arndt-University, 17489 Greifswald (Germany)

    2012-12-15

    In dense dust clouds of a dusty plasma single particle trajectories are impossible to follow due to occlusion of particles and ambiguities in particle correspondences. By stereoscopic imaging of fluorescent tracer particles, we were able to reconstruct 3D single particle trajectories within dense dust clouds. Several measurements are shown that justify to regard the tracer particles as suitable representatives for the whole dust system. A first analysis of dust density waves in dense clouds already shows that these waves exhibit three-dimensional dynamics at larger wave amplitudes that cannot be resolved by 2D imaging techniques: a broad velocity distribution perpendicular to the oscillation plane due to dust-dust collisions is seen, while the velocity distribution in the oscillation direction is bimodal and shifted due to the bulk wave propagation.

  16. A low power dual-band multi-mode RF front-end for GNSS applications

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Hao; Li Zhiqun; Wang Zhigong, E-mail: zhhseu@gmail.com [Institute of RF- and OE- ICs, Southeast University, Nanjing 210096 (China)

    2010-11-15

    A CMOS dual-band multi-mode RF front-end for the global navigation satellite system receivers of all GPS, Bei-Dou, Galileo and Glonass systems is presented. It consists of a reconfigurable low noise amplifier (LNA), a broadband active balun, a high linearity mixer and a bandgap reference (BGR) circuit. The effect of the input parasitic capacitance on the input impedance of the inductively degenerated common source LNA is analyzed in detail. By using two different LC networks at the input port and the switched capacitor at the output port, the LNA can work at two different frequency bands (1.2 GHz and 1.5 GHz) under low power consumption. The active balun uses a hybrid-connection structure to achieve high bandwidth. The mixer uses the multiple gated transistors technique to acquire a high linearity under low power consumption but does not deteriorate other performances. The measurement results of the proposed front-end achieve a noise figure of 2.1/2.0 dB, again of 33.9/33.8 dB and an input 1-dB compression point of 0/1 dBm at 1227.6/1575.42 MHz. The power consumption is about 16 mW under a 1.8 V power supply.

  17. Single-Particle Time-of-Flight Mass Spectrometry Utilizing a Femtosecond Desorption and Ionization Laser.

    Science.gov (United States)

    Zawadowicz, Maria A; Abdelmonem, Ahmed; Mohr, Claudia; Saathoff, Harald; Froyd, Karl D; Murphy, Daniel M; Leisner, Thomas; Cziczo, Daniel J

    2015-12-15

    Single-particle time-of-flight mass spectrometry has now been used since the 1990s to determine particle-to-particle variability and internal mixing state. Instruments commonly use 193 nm excimer or 266 nm frequency-quadrupled Nd:YAG lasers to ablate and ionize particles in a single step. We describe the use of a femtosecond laser system (800 nm wavelength, 100 fs pulse duration) in combination with an existing single-particle time-of-flight mass spectrometer. The goal of this project was to determine the suitability of a femtosecond laser for single-particle studies via direct comparison to the excimer laser (193 nm wavelength, ∼10 ns pulse duration) usually used with the instrument. Laser power, frequency, and polarization were varied to determine the effect on mass spectra. Atmospherically relevant materials that are often used in laboratory studies, ammonium nitrate and sodium chloride, were used for the aerosol. Detection of trace amounts of a heavy metal, lead, in an ammonium nitrate matrix was also investigated. The femtosecond ionization had a large air background not present with the 193 nm excimer and produced more multiply charged ions. Overall, we find that femtosecond laser ablation and ionization of aerosol particles is not radically different than that provided by a 193 nm excimer.

  18. Analysis and synthesis of multi-qubit, multi-mode quantum devices

    Energy Technology Data Exchange (ETDEWEB)

    Solgun, Firat

    2015-03-27

    In this thesis we propose new methods in multi-qubit multi-mode circuit quantum electrodynamics (circuit-QED) architectures. First we describe a direct parity measurement method for three qubits, which can be realized in 2D circuit-QED with a possible extension to four qubits in a 3D circuit-QED setup for the implementation of the surface code. In Chapter 3 we show how to derive Hamiltonians and compute relaxation rates of the multi-mode superconducting microwave circuits consisting of single Josephson junctions using an exact impedance synthesis technique (the Brune synthesis) and applying previous formalisms for lumped element circuit quantization. In the rest of the thesis we extend our method to multi-junction (multi-qubit) multi-mode circuits through the use of state-space descriptions which allows us to quantize any multiport microwave superconducting circuit with a reciprocal lossy impedance response.

  19. Dwell time algorithm for multi-mode optimization in manufacturing large optical mirrors

    Science.gov (United States)

    Liu, Zhenyu

    2014-08-01

    CCOS (Computer Controlled Optical Surfacing) is one of the most important method to manufacture optical surface. By controlling the dwell time of a polishing tool on the mirror we can get the desired material removal. As the optical surface becoming larger, traditional CCOS method can't meet the demand that manufacturing the mirror in higher efficiency and precision. This paper presents a new method using multi-mode optimization. By calculate the dwell time map of different tool in one optimization cycle, the larger tool and the small one have complementary advantages and obtain a global optimization for multi tool and multi-processing cycles. To calculate the dwell time of different tool at the same time we use multi-mode dwell time algorithm that based on matrix calculation. With this algorithm we did simulation experiment, the result shows using multi-mode optimization algorithm can improve the efficiency maintaining good precision.

  20. OM4 bend insensitive multi-mode fibers’ usefulness for MCM integration

    International Nuclear Information System (INIS)

    Guzowski, Bartłomiej; Lisik, Zbigniew; Tosik, Grzegorz; Ciupa, Emilia

    2012-01-01

    Highlights: ► The influence of high temperature exposure on OM4 fibers’ mechanical properties. ► Researching OM4 class fibers for use in innovative Optical Multi Chip Module. ► The influence of bending at a very small radius, up to 2 mm, on MM fibers. - Abstract: For future generations of electronic systems, a severe bottleneck is expected on the interconnection level and the use of optical interconnection is considered as one of the most promising solutions in this matter. Recent progress in fiber development resulted in new generation of optical fibers that are bend insensitive. This makes them ideal for Multi Chip Module (MCM) application. This paper focuses on OM4 bend insensitive multi-mode fibers’ usefulness for MCM integration, particularly the investigation of MM fiber loss is presented, which is influenced by bend diameter and the fiber's mechanical performance under influence of high temperature (400 °C–1000 °C adequate to MCM production process).

  1. An inductorless multi-mode RF front end for GNSS receiver in 55 nm CMOS

    Science.gov (United States)

    Yanbin, Luo; Chengyan, Ma; Yebing, Gan; Min, Qian; Tianchun, Ye

    2015-10-01

    An inductorless multi-mode RF front end for a global navigation satellite system (GNSS) receiver is presented. Unlike the traditional topology of a low noise amplifier (LNA), the inductorless current-mode noise-canceling LNA is applied in this design. The high-impedance-input radio frequency amplifier (RFA) further amplifies the GNSS signals and changes the single-end signal path into fully differential. The passive mixer down-converts the signals to the intermediate frequency (IF) band and conveys the signals to the analogue blocks. The local oscillator (LO) buffer divides the output frequency of the voltage controlled oscillator (VCO) and generates 25%-duty-cycle quadrature square waves to drive the mixer. Our measurement results display that the implemented RF front end achieves good overall performance while consuming only 6.7 mA from 1.2 V supply. The input return loss is better than -26 dB and the ultra low noise figure of 1.43 dB leads to high sensitivity of the GNSS receiver. The input 1 dB compression point is -43 dBm at the high gain of 48 dB. The designed circuit is fabricated in 55 nm CMOS technology and the die area, which is much smaller than traditional circuit, is around 220 × 280 μm2.

  2. Emissions from Ethanol-Gasoline Blends: A Single Particle Perspective

    Directory of Open Access Journals (Sweden)

    Peter H. McMurry

    2011-06-01

    Full Text Available Due to its agricultural origin and function as a fuel oxygenate, ethanol is being promoted as an alternative biomass-based fuel for use in spark ignition engines, with mandates for its use at state and regional levels. While it has been established that the addition of ethanol to a fuel reduces the particulate mass concentration in the exhaust, little attention has been paid to changes in the physicochemical properties of the emitted particles. In this work, a dynamometer-mounted GM Quad-4 spark ignition engine run without aftertreatment at 1,500 RPM and 100% load was used with four different fuel blends, containing 0, 20, 40 and 85 percent ethanol in gasoline. This allowed the effects of the fuel composition to be isolated from other effects. Instrumentation employed included two Aerosol Time-of-Flight Mass Spectrometers covering different size ranges for analysis of single particle composition, an Aethalometer for black carbon, a Scanning Mobility Particle Sizer for particle size distributions, a Photoelectric Aerosol Sensor for particle-bound polycyclic aromatic hydrocarbon (PAH species and gravimetric filter measurements for particulate mass concentrations. It was found that, under the conditions investigated here, additional ethanol content in the fuel changes the particle size distribution, especially in the accumulation mode, and decreases the black carbon and total particulate mass concentrations. The molecular weight distribution of the PAHs was found to decrease with added ethanol. However, PAHs produced from higher ethanol-content fuels are associated with NO2− (m/z—46 in the single-particle mass spectra, indicating the presence of nitro-PAHs. Compounds associated with the gasoline (e.g., sulfur-containing species are diminished due to dilution as ethanol is added to the fuel relative to those associated with the lubricating oil (e.g., calcium, zinc, phosphate in the single particle spectra. These changes have potential

  3. Decay properties of high-lying single-particles modes

    Science.gov (United States)

    Beaumel, D.; Fortier, S.; Galès, S.; Guillot, J.; Langevin-Joliot, H.; Laurent, H.; Maison, J. M.; Vernotte, J.; Bordewijck, J.; Brandenburg, S.; Krasznahorkay, A.; Crawley, G. M.; Massolo, C. P.; Renteria, M.; Khendriche, A.

    1996-02-01

    The neutron decay of high-lying single-particle states in 64Ni, 90Zr, 120Sn and 208Pb excited by means of the (α, 3He) reaction has been investigated at 120 MeV incident energy using the multidetector EDEN. The characteristics of this reaction are studied using inclusive spectra and angular correlation analysis. The structure located between 11 and 15 MeV in 91Zr, and between 8 and 12 MeV excitation energy in 209Pb display large departures from a pure statistical decay. The corresponding non-statistical branching ratios are compared with the results of two theoretical calculations.

  4. Real stabilization method for nuclear single-particle resonances

    International Nuclear Information System (INIS)

    Zhang Li; Zhou Shangui; Meng Jie; Zhao Enguang

    2008-01-01

    We develop the real stabilization method within the framework of the relativistic mean-field (RMF) model. With the self-consistent nuclear potentials from the RMF model, the real stabilization method is used to study single-particle resonant states in spherical nuclei. As examples, the energies, widths, and wave functions of low-lying neutron resonant states in 120 Sn are obtained. These results are compared with those from the scattering phase-shift method and the analytic continuation in the coupling constant approach and satisfactory agreements are found

  5. Single-particle cryo-EM at crystallographic resolution

    Science.gov (United States)

    Cheng, Yifan

    2015-01-01

    Until only a few years ago, single-particle electron cryo-microscopy (cryo-EM) was usually not the first choice for many structural biologists due to its limited resolution in the range of nanometer to subnanometer. Now, this method rivals X-ray crystallography in terms of resolution and can be used to determine atomic structures of macromolecules that are either refractory to crystallization or difficult to crystallize in specific functional states. In this review, I discuss the recent breakthroughs in both hardware and software that transformed cryo-microscopy, enabling understanding of complex biomolecules and their functions at atomic level. PMID:25910205

  6. Coupled cluster approach to the single-particle Green's function

    International Nuclear Information System (INIS)

    Nooijen, M.; Snijders, J.G.

    1992-01-01

    Diagrammatic and coupled cluster techniques are used to develop an approach to the single-particle Green's function G which concentrates on G directly rather than first approximating the irreducible self-energy and then solving Dyson's equation. As a consequence the ionization and attachment parts of the Green's function satisfy completely decoupled sets of equations. The proposed coupled cluster Green's function method (CCGF) is intimately connected to both coupled cluster linear response theory (CCLRT) and the normal coupled cluster method (NCCM). These relations are discussed in detail

  7. A multi-mode operation control strategy for flexible microgrid based on sliding-mode direct voltage and hierarchical controls.

    Science.gov (United States)

    Zhang, Qinjin; Liu, Yancheng; Zhao, Youtao; Wang, Ning

    2016-03-01

    Multi-mode operation and transient stability are two problems that significantly affect flexible microgrid (MG). This paper proposes a multi-mode operation control strategy for flexible MG based on a three-layer hierarchical structure. The proposed structure is composed of autonomous, cooperative, and scheduling controllers. Autonomous controller is utilized to control the performance of the single micro-source inverter. An adaptive sliding-mode direct voltage loop and an improved droop power loop based on virtual negative impedance are presented respectively to enhance the system disturbance-rejection performance and the power sharing accuracy. Cooperative controller, which is composed of secondary voltage/frequency control and phase synchronization control, is designed to eliminate the voltage/frequency deviations produced by the autonomous controller and prepare for grid connection. Scheduling controller manages the power flow between the MG and the grid. The MG with the improved hierarchical control scheme can achieve seamless transitions from islanded to grid-connected mode and have a good transient performance. In addition the presented work can also optimize the power quality issues and improve the load power sharing accuracy between parallel VSIs. Finally, the transient performance and effectiveness of the proposed control scheme are evaluated by theoretical analysis and simulation results. Copyright © 2015 ISA. Published by Elsevier Ltd. All rights reserved.

  8. A triple hybrid micropower generator with simultaneous multi-mode energy harvesting

    Science.gov (United States)

    Uluşan, H.; Chamanian, S.; Pathirana, W. P. M. R.; Zorlu, Ö.; Muhtaroğlu, A.; Külah, H.

    2018-01-01

    This study presents a triple hybrid energy harvesting system that combines harvested power from thermoelectric (TE), vibration-based electromagnetic (EM) and piezoelectric (PZT) harvesters into a single DC supply. A power management circuit is designed and implemented in 180 nm standard CMOS technology based on the distinct requirements of each harvester, and is terminated with a Schottky diode to avoid reverse current flow. The system topology hence supports simultaneous power generation and delivery from low and high frequency vibrations as well as temperature differences in the environment. The ultra-low DC voltage harvested from TE generator is boosted with a cross-coupled charge-pump driven by an LC oscillator with fully-integrated center-tapped differential inductors. The EM harvester output was rectified with a self-powered and low drop-out AC/DC doubler circuit. The PZT interface electronics benefits from peak-to-peak cycle of the harvested voltage through a negative voltage converter followed by synchronous power extraction and DC-to-DC conversion through internal switches, and an external inductor. The hybrid system was tested with a wearable in-house EM energy harvester placed wrist of a jogger, a commercial low volume PZT harvester, and DC supply as the TE generator output. The system generates more than 1.2 V output for load resistances higher than 50 kΩ, which corresponds to 24 μW to power wearable sensors. Simultaneous multi-mode operation achieves higher voltage and power compared to stand-alone harvesting circuits, and generates up to 110 μW of output power. This is the first hybrid harvester circuit that simultaneously extracts energy from three independent sources, and delivers a single DC output.

  9. High resolution single particle refinement in EMAN2.1.

    Science.gov (United States)

    Bell, James M; Chen, Muyuan; Baldwin, Philip R; Ludtke, Steven J

    2016-05-01

    EMAN2.1 is a complete image processing suite for quantitative analysis of grayscale images, with a primary focus on transmission electron microscopy, with complete workflows for performing high resolution single particle reconstruction, 2-D and 3-D heterogeneity analysis, random conical tilt reconstruction and subtomogram averaging, among other tasks. In this manuscript we provide the first detailed description of the high resolution single particle analysis pipeline and the philosophy behind its approach to the reconstruction problem. High resolution refinement is a fully automated process, and involves an advanced set of heuristics to select optimal algorithms for each specific refinement task. A gold standard FSC is produced automatically as part of refinement, providing a robust resolution estimate for the final map, and this is used to optimally filter the final CTF phase and amplitude corrected structure. Additional methods are in-place to reduce model bias during refinement, and to permit cross-validation using other computational methods. Copyright © 2016 Elsevier Inc. All rights reserved.

  10. Single-particle absorption spectroscopy by photothermal contrast.

    Science.gov (United States)

    Yorulmaz, Mustafa; Nizzero, Sara; Hoggard, Anneli; Wang, Lin-Yung; Cai, Yi-Yu; Su, Man-Nung; Chang, Wei-Shun; Link, Stephan

    2015-05-13

    Removing effects of sample heterogeneity through single-molecule and single-particle techniques has advanced many fields. While background free luminescence and scattering spectroscopy is widely used, recording the absorption spectrum only is rather difficult. Here we present an approach capable of recording pure absorption spectra of individual nanostructures. We demonstrate the implementation of single-particle absorption spectroscopy on strongly scattering plasmonic nanoparticles by combining photothermal microscopy with a supercontinuum laser and an innovative calibration procedure that accounts for chromatic aberrations and wavelength-dependent excitation powers. Comparison of the absorption spectra to the scattering spectra of the same individual gold nanoparticles reveals the blueshift of the absorption spectra, as predicted by Mie theory but previously not detectable in extinction measurements that measure the sum of absorption and scattering. By covering a wavelength range of 300 nm, we are furthermore able to record absorption spectra of single gold nanorods with different aspect ratios. We find that the spectral shift between absorption and scattering for the longitudinal plasmon resonance decreases as a function of nanorod aspect ratio, which is in agreement with simulations.

  11. Single-particle excitations in disordered Weyl fluids

    Science.gov (United States)

    Pixley, J. H.; Chou, Yang-Zhi; Goswami, Pallab; Huse, David A.; Nandkishore, Rahul; Radzihovsky, Leo; Das Sarma, S.

    2017-06-01

    We theoretically study the single-particle Green function of a three-dimensional disordered Weyl semimetal using a combination of techniques. These include analytic T -matrix and renormalization group methods with complementary regimes of validity and an exact numerical approach based on the kernel polynomial technique. We show that at any nonzero disorder, Weyl excitations are not ballistic: They instead have a nonzero linewidth that for weak short-range disorder arises from nonperturbative resonant impurity scattering. Perturbative approaches find a quantum critical point between a semimetal and a metal at a finite disorder strength, but this transition is avoided due to nonperturbative effects. At moderate disorder strength and intermediate energies the avoided quantum critical point renormalizes the scaling of single-particle properties. In this regime we compute numerically the anomalous dimension of the fermion field and find η =0.13 ±0.04 , which agrees well with a renormalization group analysis (η =0.125 ). Our predictions can be directly tested by ARPES and STM measurements in samples dominated by neutral impurities.

  12. Efficient multi-mode to single-mode coupling in a photonic lantern

    DEFF Research Database (Denmark)

    Noordegraaf, Danny; Skovgaard, Peter M.; Nielsen, Martin D.

    2009-01-01

    We demonstrate the fabrication of a high performance multi-mode (MM) to single-mode (SM) splitter or “photonic lantern”, first described by Leon-Saval et al. (2005). Our photonic lantern is a solid all-glass version, and we show experimentally that this device can be used to achieve efficient and...

  13. A quantum-classical simulation of a multi-surface multi-mode ...

    Indian Academy of Sciences (India)

    J. Chem. Sci. Vol. 124, No. 1, January 2012, pp. 51–58. c Indian Academy of Sciences. A quantum-classical simulation of a multi-surface multi-mode nuclear dynamics on C6H. +. 6 incorporating degeneracy among electronic states. #. SUBHANKAR SARDAR and SATRAJIT ADHIKARI. ∗. Department of Physical Chemistry, ...

  14. An Adaptive Large Neighborhood Search Algorithm for the Multi-mode RCPSP

    DEFF Research Database (Denmark)

    Muller, Laurent Flindt

    We present an Adaptive Large Neighborhood Search algorithm for the Multi-mode Resource-Constrained Project Scheduling Problem (MRCPSP). We incorporate techniques for deriving additional precedence relations and propose a new method, so-called mode-diminution, for removing modes during execution...

  15. Multi-Mode, Multi-Band Active-RC Filterand Tuning Circuits for SDR Applications

    OpenAIRE

    Lee, Kang-Yoon

    2010-01-01

    The CMOS multi-mode, multi-band low-pass filter and complex baseband filter are presented. Capacitors and resistors were shared to minimize the area. Proposed two-step tuning method can reduce the number of switches and thus, can reduce the noise and the area.

  16. Phase-coexistence simulations of fluid mixtures by the Markov Chain Monte Carlo method using single-particle models

    KAUST Repository

    Li, Jun

    2013-09-01

    We present a single-particle Lennard-Jones (L-J) model for CO2 and N2. Simplified L-J models for other small polyatomic molecules can be obtained following the methodology described herein. The phase-coexistence diagrams of single-component systems computed using the proposed single-particle models for CO2 and N2 agree well with experimental data over a wide range of temperatures. These diagrams are computed using the Markov Chain Monte Carlo method based on the Gibbs-NVT ensemble. This good agreement validates the proposed simplified models. That is, with properly selected parameters, the single-particle models have similar accuracy in predicting gas-phase properties as more complex, state-of-the-art molecular models. To further test these single-particle models, three binary mixtures of CH4, CO2 and N2 are studied using a Gibbs-NPT ensemble. These results are compared against experimental data over a wide range of pressures. The single-particle model has similar accuracy in the gas phase as traditional models although its deviation in the liquid phase is greater. Since the single-particle model reduces the particle number and avoids the time-consuming Ewald summation used to evaluate Coulomb interactions, the proposed model improves the computational efficiency significantly, particularly in the case of high liquid density where the acceptance rate of the particle-swap trial move increases. We compare, at constant temperature and pressure, the Gibbs-NPT and Gibbs-NVT ensembles to analyze their performance differences and results consistency. As theoretically predicted, the agreement between the simulations implies that Gibbs-NVT can be used to validate Gibbs-NPT predictions when experimental data is not available. © 2013 Elsevier Inc.

  17. Multi-mode optical fibers for connecting space-based spectrometers

    Science.gov (United States)

    Roberts, W. T.; Lindenmisth, C. A.; Bender, S.; Miller, E. A.; Motts, E.; Ott, M.; LaRocca, F.; Thomes, J.

    2017-11-01

    significantly smaller, less massive and less robust. Large core multi-mode optical fibers are often used to accommodate the optical connection of the two separated portions of such instrumentation. In some cases, significant throughput efficiency improvement can be realized by judiciously orienting the strands of multi-fiber cable, close-bunching them to accommodate a tight focus of the optical system on the optical side of the connection, and splaying them out linearly along a spectrometer slit on the other end. For such instrumentation to work effectively in identifying elements and molecules, and especially to produce accurate quantitative results, the spectral throughput of the optical fiber connection must be consistent over varying temperatures, over the range of motion of the optical head (and it's implied optical cable stresses), and over angle-aperture invariant of the total system. While the first two of these conditions have been demonstrated[4], spectral observations of the latter present a cause for concern, and may have an impact on future design of fiber-connected LIBS and Raman spectroscopy instruments. In short, we have observed that the shape of the spectral efficiency curve of a large multi-mode core optical fiber changes as a function of input angle.

  18. Optimizing strategy software for repetitive construction projects within multi-mode resources

    Directory of Open Access Journals (Sweden)

    Remon Fayek Aziz

    2013-09-01

    Full Text Available Estimating tender data for specific project is the most essential part in construction areas as of contractor’s view such as: proposed project duration with corresponding gross value and cash flows. This paper focuses on how to calculate tender data using Optimizing Strategy Software (OSS for repetitive construction projects with identical activity’s duration in case of single number of crew such as: project duration, project/bid price, project maximum working capital, and project net present value of the studied project. A simplified multi-objective optimization software (OSS will be presented that creates best tender data to contractor compared with more feasible options generated from multi-mode resources in a given project. OSS is intended to give more scenarios which provide practical support for typical construction contractors who need to optimize resource utilization in order to minimize project duration, project/bid price, and project maximum working capital while maximizing its net present value simultaneously. OSS is designed by java programing code system to provide a number of new and unique capabilities, including: (1 Ranking the obtained optimal plans according to a set of planner specified weights representing the relative importance of duration, price, maximum working capital and net present value in the analyzed project; (2 Visualizing and viewing the generated optimal trade-off; and (3 Providing seamless integration with available project management calculations. In order to provide the aforementioned capabilities of OSS, the system is implemented and developed in four main modules: (1 A user interface module; (2 A database module; (3 A running module; (4 A connecting module. At the end of the paper, an illustrative example will be presented to demonstrate and verify the applications of the proposed software (OSS to an optimization expressway of repetitive construction project.

  19. Decay properties of high-lying single-particles modes

    Energy Technology Data Exchange (ETDEWEB)

    Beaumel, D. [Institut de Physique Nucleaire, 91 - Orsay (France); Fortier, S. [Institut de Physique Nucleaire, 91 - Orsay (France); Gales, S. [Institut de Physique Nucleaire, 91 - Orsay (France); Guillot, J. [Institut de Physique Nucleaire, 91 - Orsay (France); Langevin-Joliot, H. [Institut de Physique Nucleaire, 91 - Orsay (France); Laurent, H. [Institut de Physique Nucleaire, 91 -Orsay (France); Maison, J.M. [Institut de Physique Nucleaire, 91 - Orsay (France); Vernotte, J. [Institut de Physique Nucleaire, 91 - Orsay (France); Bordewijck, J. [Kernfysisch Versneller Instituut, 9747 Groningen (Netherlands); Brandenburg, S. [Kernfysisch Versneller Instituut, 9747 Groningen (Netherlands); Krasznahorkay, A. [Kernfysisch Versneller Instituut, 9747 Groningen (Netherlands); Crawley, G.M. [NSCL, Michigan State University, East Lansing, MI 48824 (United States); Massolo, C.P. [Universitad Nacional de La Plata, 1900 La Plata (Argentina); Renteria, M. [Universitad Nacional de La Plata, 1900 La Plata (Argentina); Khendriche, A. [University of Tizi-Ouzou, Tizi-Ouzou (Algeria)

    1996-03-18

    The neutron decay of high-lying single-particle states in {sup 64}Ni, {sup 90}Zr, {sup 120}Sn and {sup 208}Pb excited by means of the ({alpha},{sup 3}He) reaction has been investigated at 120 MeV incident energy using the multidetector EDEN. The characteristics of this reaction are studied using inclusive spectra and angular correlation analysis. The structure located between 11 and 15 MeV in {sup 91}Zr, and between 8 and 12 MeV excitation energy in {sup 209}Pb display large departures from a pure statistical decay. The corresponding non-statistical branching ratios are compared with the results of two theoretical calculations. (orig.).

  20. Magnetophoretic circuits for digital control of single particles and cells

    Science.gov (United States)

    Lim, Byeonghwa; Reddy, Venu; Hu, Xinghao; Kim, Kunwoo; Jadhav, Mital; Abedini-Nassab, Roozbeh; Noh, Young-Woock; Lim, Yong Taik; Yellen, Benjamin B.; Kim, Cheolgi

    2014-05-01

    The ability to manipulate small fluid droplets, colloidal particles and single cells with the precision and parallelization of modern-day computer hardware has profound applications for biochemical detection, gene sequencing, chemical synthesis and highly parallel analysis of single cells. Drawing inspiration from general circuit theory and magnetic bubble technology, here we demonstrate a class of integrated circuits for executing sequential and parallel, timed operations on an ensemble of single particles and cells. The integrated circuits are constructed from lithographically defined, overlaid patterns of magnetic film and current lines. The magnetic patterns passively control particles similar to electrical conductors, diodes and capacitors. The current lines actively switch particles between different tracks similar to gated electrical transistors. When combined into arrays and driven by a rotating magnetic field clock, these integrated circuits have general multiplexing properties and enable the precise control of magnetizable objects.

  1. Classification using diffraction patterns for single-particle analysis

    Energy Technology Data Exchange (ETDEWEB)

    Hu, Hongli; Zhang, Kaiming [Department of Biophysics, the Health Science Centre, Peking University, Beijing 100191 (China); Meng, Xing, E-mail: xmeng101@gmail.com [Wadsworth Centre, New York State Department of Health, Albany, New York 12201 (United States)

    2016-05-15

    An alternative method has been assessed; diffraction patterns derived from the single particle data set were used to perform the first round of classification in creating the initial averages for proteins data with symmetrical morphology. The test protein set was a collection of Caenorhabditis elegans small heat shock protein 17 obtained by Cryo EM, which has a tetrahedral (12-fold) symmetry. It is demonstrated that the initial classification on diffraction patterns is workable as well as the real-space classification that is based on the phase contrast. The test results show that the information from diffraction patterns has the enough details to make the initial model faithful. The potential advantage using the alternative method is twofold, the ability to handle the sets with poor signal/noise or/and that break the symmetry properties. - Highlights: • New classification method. • Create the accurate initial model. • Better in handling noisy data.

  2. Classification using diffraction patterns for single-particle analysis

    International Nuclear Information System (INIS)

    Hu, Hongli; Zhang, Kaiming; Meng, Xing

    2016-01-01

    An alternative method has been assessed; diffraction patterns derived from the single particle data set were used to perform the first round of classification in creating the initial averages for proteins data with symmetrical morphology. The test protein set was a collection of Caenorhabditis elegans small heat shock protein 17 obtained by Cryo EM, which has a tetrahedral (12-fold) symmetry. It is demonstrated that the initial classification on diffraction patterns is workable as well as the real-space classification that is based on the phase contrast. The test results show that the information from diffraction patterns has the enough details to make the initial model faithful. The potential advantage using the alternative method is twofold, the ability to handle the sets with poor signal/noise or/and that break the symmetry properties. - Highlights: • New classification method. • Create the accurate initial model. • Better in handling noisy data.

  3. Reconstructing an icosahedral virus from single-particle diffraction experiments

    Science.gov (United States)

    Saldin, D. K.; Poon, H.-C.; Schwander, P.; Uddin, M.; Schmidt, M.

    2011-08-01

    The first experimental data from single-particle scattering experiments from free electron lasers (FELs) are now becoming available. The first such experiments are being performed on relatively large objects such as viruses, which produce relatively low-resolution, low-noise diffraction patterns in so-called ``diffract-and-destroy'' experiments. We describe a very simple test on the angular correlations of measured diffraction data to determine if the scattering is from an icosahedral particle. If this is confirmed, the efficient algorithm proposed can then combine diffraction data from multiple shots of particles in random unknown orientations to generate a full 3D image of the icosahedral particle. We demonstrate this with a simulation for the satellite tobacco necrosis virus (STNV), the atomic coordinates of whose asymmetric unit is given in Protein Data Bank entry 2BUK.

  4. Single particle analysis with a 3600 light scattering photometer

    International Nuclear Information System (INIS)

    Bartholdi, M.F.

    1979-06-01

    Light scattering by single spherical homogeneous particles in the diameter range 1 to 20 μm and relative refractive index 1.20 is measured. Particle size of narrowly dispersed populations is determined and a multi-modal dispersion of five components is completely analyzed. A 360 0 light scattering photometer for analysis of single particles has been designed and developed. A fluid stream containing single particles intersects a focused laser beam at the primary focal point of an ellipsoidal reflector ring. The light scattered at angles theta = 2.5 0 to 177.5 0 at phi = 0 0 and 180 0 is reflected onto a circular array of photodiodes. The ellipsoidal reflector is situated in a chamber filled with fluid matching that of the stream to minimize refracting and reflecting interfaces. The detector array consists of 60 photodiodes each subtending 3 0 in scattering angle on 6 0 centers around 360 0 . 32 measurements on individual particles can be acquired at rates of 500 particles per second. The intensity and angular distribution of light scattered by spherical particles are indicative of size and relative refractive index. Calculations, using Lorenz--Mie theory, of differential scattering patterns integrated over angle corresponding to the detector geometry determined the instrument response to particle size. From this the expected resolution and experimental procedures are determined.Ultimately, the photometer will be utilized for identification and discrimination of biological cells based on the sensitivity of light scattering to size, shape, refractive index differences, internal granularity, and other internal morphology. This study has demonstrated the utility of the photometer and indicates potential for application to light scattering studies of biological cells

  5. Hybrid PD and effective multi-mode positive position feedback control for slewing and vibration suppression of a smart flexible manipulator

    International Nuclear Information System (INIS)

    Lou, Jun-qiang; Wei, Yan-ding; Yang, Yi-ling; Xie, Feng-ran

    2015-01-01

    A hybrid control strategy for slewing and vibration suppression of a smart flexible manipulator is presented in this paper. It consists of a proportional derivative controller to realize motion control, and an effective multi-mode positive position feedback (EMPPF) controller to suppress the multi-mode vibration. Rather than treat each mode equally as the standard multi-mode PPF, the essence of the EMPPF is that control forces of different modes are applied according to the mode parameters of the respective modes, so the vibration modes with less vibration energy receive fewer control forces. Stability conditions for the close loop system are established through stability analysis. Optimal parameters of the EMPPF controller are obtained using the method of root locus analysis. The performance of the proposed strategy is demonstrated by simulation and experiments. Experimental results show that the first two vibration modes of the manipulator are effectively suppressed. The setting time of the setup descends approximately 55%, reaching 3.12 s from 5.67 s. (paper)

  6. Development and characterization of a single particle laser ablation mass spectrometer (SPLAM for organic aerosol studies

    Directory of Open Access Journals (Sweden)

    F. Gaie-Levrel

    2012-01-01

    Full Text Available A single particle instrument was developed for real-time analysis of organic aerosol. This instrument, named Single Particle Laser Ablation Mass Spectrometry (SPLAM, samples particles using an aerodynamic lens system for which the theoretical performances were calculated. At the outlet of this system, particle detection and sizing are realized by using two continuous diode lasers operating at λ = 403 nm. Polystyrene Latex (PSL, sodium chloride (NaCl and dioctylphtalate (DOP particles were used to characterize and calibrate optical detection of SPLAM. The optical detection limit (DL and detection efficiency (DE were determined using size-selected DOP particles. The DE ranges from 0.1 to 90% for 100 and 350 nm DOP particles respectively and the SPLAM instrument is able to detect and size-resolve particles as small as 110–120 nm. During optical detection, particle scattered light from the two diode lasers, is detected by two photomultipliers and the detected signals are used to trigger UV excimer laser (λ = 248 nm used for one-step laser desorption ionization (LDI of individual aerosol particles. The formed ions are analyzed by a 1 m linear time-of-flight mass spectrometer in order to access to the chemical composition of individual particles. The TOF-MS detection limit for gaseous aromatic compounds was determined to be 0.85 × 10−15 kg (∼4 × 103 molecules. DOP particles were also used to test the overall operation of the instrument. The analysis of a secondary organic aerosol, formed in a smog chamber by the ozonolysis of indene, is presented as a first application of the instrument. Single particle mass spectra were obtained with an effective hit rate of 8%. Some of these mass spectra were found to be very different from one particle to another possibly reflecting chemical differences within the investigated indene SOA particles. Our study shows that an exhaustive statistical analysis, over hundreds of particles

  7. Color digital holography using speckle illumination by means of a multi-mode fiber

    Science.gov (United States)

    Funamizu, Hideki; Shimoma, Shohei; Aizu, Yoshihisa

    2014-02-01

    We present color digital holography using speckle illumination by means of a multi-mode fiber. In this technique, speckle fields emitted from the fiber are used as both a reference wave and a wavefront illuminating an object. For three wavelengths, the interference patterns of two coherent waves are recorded as digital holograms on a CCD camera. A speckle method is used for suppressing DC terms and reducing a twin image in an in-line color digital holography. The speckle fields are changed by vibrating the multi-mode fiber using a vibrator, and a number of holograms are acquired to average reconstructed images. The dependence of the averaged number of holograms on color quality of reconstructed images is evaluated by chromaticity coordinates and color differences in colorimetry.

  8. Improved resistive shunt by means of negative capacitance: new circuit, performances and multi-mode control

    OpenAIRE

    BERARDENGO, Marta; THOMAS, Olivier; GIRAUD-AUDINE, Christophe; MANZONI, Stefano

    2016-01-01

    This paper deals with vibration control by means of piezoelectric patches shunted with electrical impedances made up by a resistance and a negative capacitance. The paper analyses most of the possible layouts by which a negative capacitance can be built and shows that a common mathematical description is possible. This allows closed formulations to be found in order to optimise the electrical network for mono- and multi-mode control. General analytical formulations are obtained to estimate th...

  9. Unconventional geometric logic gate in a strong-driving-assisted multi-mode cavity

    International Nuclear Information System (INIS)

    Chang-Ning, Pan; Di-Wu, Yang; Xue-Hui, Zhao; Mao-Fa, Fang

    2010-01-01

    We propose a scheme to implement an unconventional geometric logic gate separately in a two-mode cavity and a multi-mode cavity assisted by a strong classical driving field. The effect of the cavity decay is included in the investigation. The numerical calculation is carried out, and the result shows that our scheme is more tolerant to cavity decay than the previous one because the time consumed for finishing the logic gate is doubly reduced. (general)

  10. 3D dual-virtual-pinhole assisted single particle tracking microscopy

    International Nuclear Information System (INIS)

    Ma, Ye; Wang, Yifan; Zhou, Xin; Kuang, Cuifang; Liu, Xu

    2014-01-01

    We propose a novel approach for high-speed, three-dimensional single particle tracking (SPT), which we refer to as dual-virtual-pinhole assisted single particle tracking microscopy (DVPaSPTM). DVPaSPTM system can obtain axial information of the sample without optical or mechanical depth scanning, so as to offer numbers of advantages including faster imaging, improved efficiency and a great reduction of photobleaching and phototoxicity. In addition, by the use of the dual-virtual-pinhole, the effect that the quantum yield exerts to the fluorescent signal can be eliminated, which makes the measurement independent of the surroundings and increases the accuracy of the result. DVPaSPTM system measures the intensity within different virtual pinholes of which the radii are given by the host computer. Axial information of fluorophores can be measured by the axial response curve through the ratio of intensity signals. We demonstrated the feasibility of the proposed method by a series of experiments. Results showed that the standard deviation of the axial measurement was 19.2 nm over a 2.5 μm range with 30 ms temporal resolution. (papers)

  11. The single-particle microbeam facility at CEA-Saclay

    Energy Technology Data Exchange (ETDEWEB)

    Khodja, H. [DSM/IRAMIS/SIS2M, CEA Saclay, F-91191 Gif-sur-Yvette Cedex (France); CNRS, UMR9956, Laboratoire Pierre Suee, F-91191 Gif-sur-Yvette Cedex (France)], E-mail: hicham.khodja@cea.fr; Hanot, M.; Carriere, M.; Hoarau, J. [DSM/IRAMIS/SIS2M, CEA Saclay, F-91191 Gif-sur-Yvette Cedex (France); CNRS, UMR9956, Laboratoire Pierre Suee, F-91191 Gif-sur-Yvette Cedex (France); Angulo, J.F. [DSV, IRCM, SRO, Laboratoire de Genetique de la Radiosensibilite, F-92265 Fontenay aux Roses (France)

    2009-06-15

    Low dose and non-targeted effect studies continue to attract the attention of a growing number of radiobiologists. Experimental setups based on light ion microbeams constitute a tool of choice for this kind of investigations. However, a careful attention must be given to experimental conditions, as setup-induced stress levels should be well below those induced by the irradiation itself. Here, we present the current status of the single-particle microbeam facility that has been developed these last years at the nuclear microprobe of Saclay. The driving idea was to build a facility in which local irradiation studies are performed in an environment close to cellular biology standards. This facility includes unique features, such as (i) a compact setup that allows easy access and vertical irradiation mode, (ii) a collimated beam that can be mechanically positioned under the desired cells at a very fast speed, avoiding the requirement of a focusing element and (iii) a controlled environment (temperature, CO{sub 2}, humidity) that allows performing of very long term experiments on cultured cells. Fluorescent techniques are implemented and permit in situ monitoring of cellular responses to irradiations. Several radiobiological studies are already underway and this will be illustrated with recent results regarding DNA damage and reactive oxygen species signaling time courses following targeted irradiations.

  12. Single particle raster image analysis of diffusion for particle mixtures.

    Science.gov (United States)

    Longfils, M; Röding, M; Altskär, A; Schuster, E; Lorén, N; Särkkä, A; Rudemo, M

    2018-03-01

    Recently we complemented the raster image correlation spectroscopy (RICS) method of analysing raster images via estimation of the image correlation function with the method single particle raster image analysis (SPRIA). In SPRIA, individual particles are identified and the diffusion coefficient of each particle is estimated by a maximum likelihood method. In this paper, we extend the SPRIA method to analyse mixtures of particles with a finite set of diffusion coefficients in a homogeneous medium. In examples with simulated and experimental data with two and three different diffusion coefficients, we show that SPRIA gives accurate estimates of the diffusion coefficients and their proportions. A simple technique for finding the number of different diffusion coefficients is also suggested. Further, we study the use of RICS for mixtures with two different diffusion coefficents and investigate, by plotting level curves of the correlation function, how large the quotient between diffusion coefficients needs to be in order to allow discrimination between models with one and two diffusion coefficients. We also describe a minor correction (compared to published papers) of the RICS autocorrelation function. © 2017 The Authors Journal of Microscopy © 2017 Royal Microscopical Society.

  13. Single particle composition measurements of artificial Calcium Carbonate aerosols

    Science.gov (United States)

    Zorn, S. R.; Mentel, T. F.; Schwinger, T.; Croteau, P. L.; Jayne, J.; Worsnop, D. R.; Trimborn, A.

    2012-12-01

    Mineral dust, with an estimated total source from natural and anthropogenic emissions of up to 2800 Tg/yr, is one of the two largest contributors to total aerosol mass, with only Sea salt having a similar source strength (up to 2600 Tg/yr). The composition of dust particles varies strongly depending on the production process and, most importantly, the source location. Therefore, the composition of single dust particles can be used both to trace source regions of air masses as well as to identify chemical aging processes. Here we present results of laboratory studies on generating artificial calcium carbonate (CaCO3) particles, a model compound for carbonaceous mineral dust particles. Particles were generated by atomizing an aqueous hydrogen carbonate solution. Water was removed using a silica diffusion dryer., then the particles were processed in an oven at temperatures up to 900°C, converting the hydrogen carbonate to its anhydrous form. The resulting aerosol was analyzed using an on-line single particle laser ablation aerosol particle time-of-flight mass spectrometer (LAAPTOF). The results confirm the conversion to calcium carbonate, and validate that the produced particles indeed can be used as a model compound for carbonaceous dust aerosols.

  14. Automated single particle detection and tracking for large microscopy datasets.

    Science.gov (United States)

    Wilson, Rhodri S; Yang, Lei; Dun, Alison; Smyth, Annya M; Duncan, Rory R; Rickman, Colin; Lu, Weiping

    2016-05-01

    Recent advances in optical microscopy have enabled the acquisition of very large datasets from living cells with unprecedented spatial and temporal resolutions. Our ability to process these datasets now plays an essential role in order to understand many biological processes. In this paper, we present an automated particle detection algorithm capable of operating in low signal-to-noise fluorescence microscopy environments and handling large datasets. When combined with our particle linking framework, it can provide hitherto intractable quantitative measurements describing the dynamics of large cohorts of cellular components from organelles to single molecules. We begin with validating the performance of our method on synthetic image data, and then extend the validation to include experiment images with ground truth. Finally, we apply the algorithm to two single-particle-tracking photo-activated localization microscopy biological datasets, acquired from living primary cells with very high temporal rates. Our analysis of the dynamics of very large cohorts of 10 000 s of membrane-associated protein molecules show that they behave as if caged in nanodomains. We show that the robustness and efficiency of our method provides a tool for the examination of single-molecule behaviour with unprecedented spatial detail and high acquisition rates.

  15. Simulating Biomass Fast Pyrolysis at the Single Particle Scale

    Energy Technology Data Exchange (ETDEWEB)

    Ciesielski, Peter [National Renewable Energy Laboratory (NREL); Wiggins, Gavin [ORNL; Daw, C Stuart [ORNL; Jakes, Joseph E. [U.S. Forest Service, Forest Products Laboratory, Madison, Wisconsin, USA

    2017-07-01

    Simulating fast pyrolysis at the scale of single particles allows for the investigation of the impacts of feedstock-specific parameters such as particle size, shape, and species of origin. For this reason particle-scale modeling has emerged as an important tool for understanding how variations in feedstock properties affect the outcomes of pyrolysis processes. The origins of feedstock properties are largely dictated by the composition and hierarchical structure of biomass, from the microstructural porosity to the external morphology of milled particles. These properties may be accounted for in simulations of fast pyrolysis by several different computational approaches depending on the level of structural and chemical complexity included in the model. The predictive utility of particle-scale simulations of fast pyrolysis can still be enhanced substantially by advancements in several areas. Most notably, considerable progress would be facilitated by the development of pyrolysis kinetic schemes that are decoupled from transport phenomena, predict product evolution from whole-biomass with increased chemical speciation, and are still tractable with present-day computational resources.

  16. Pairing in the BCS and LN approximations using continuum single particle level density

    Energy Technology Data Exchange (ETDEWEB)

    Id Betan, R.M., E-mail: idbetan@ifir-conicet.gov.ar [Instituto de Física Rosario (CONICET-UNR), Bv. 27 de Febrero 210 bis, S2000EZP Rosario (Argentina); Facultad de Ciencias Exactas, Ingeniería y Agrimensura (UNR), Av. Pellegrini 250, S2000BTP Rosario (Argentina); Instituto de Estudios Nucleares y Radiaciones Ionizantes (UNR), Riobamba y Berutti, S2000EKA Rosario (Argentina); Repetto, C.E. [Instituto de Física Rosario (CONICET-UNR), Bv. 27 de Febrero 210 bis, S2000EZP Rosario (Argentina); Facultad de Ciencias Exactas, Ingeniería y Agrimensura (UNR), Av. Pellegrini 250, S2000BTP Rosario (Argentina)

    2017-04-15

    Understanding the properties of drip line nuclei requires to take into account the correlations with the continuum spectrum of energy of the system. This paper has the purpose to show that the continuum single particle level density is a convenient way to consider the pairing correlation in the continuum. Isospin mean-field and isospin pairing strength are used to find the Bardeen–Cooper–Schrieffer (BCS) and Lipkin–Nogami (LN) approximate solutions of the pairing Hamiltonian. Several physical properties of the whole chain of the Tin isotope, as gap parameter, Fermi level, binding energy, and one- and two-neutron separation energies, were calculated and compared with other methods and with experimental data when they exist. It is shown that the use of the continuum single particle level density is an economical way to include explicitly the correlations with the continuum spectrum of energy in large scale mass calculation. It is also shown that the computed properties are in good agreement with experimental data and with more sophisticated treatment of the pairing interaction.

  17. Effect of single-particle splitting in the exact wave function of the isovectorial pairing Hamiltonian

    International Nuclear Information System (INIS)

    Lerma H, S.

    2010-01-01

    The structure of the exact wave function of the isovectorial pairing Hamiltonian with nondegenerate single-particle levels is discussed. The way that the single-particle splittings break the quartet condensate solution found for N=Z nuclei in a single degenerate level is established. After a brief review of the exact solution, the structure of the wave function is analyzed and some particular cases are considered where a clear interpretation of the wave function emerges. An expression for the exact wave function in terms of the isospin triplet of pair creators is given. The ground-state wave function is analyzed as a function of pairing strength, for a system of four protons and four neutrons. For small and large values of the pairing strength a dominance of two-pair (quartets) scalar couplings is found, whereas for intermediate values enhancements of the nonscalar couplings are obtained. A correlation of these enhancements with the creation of Cooper-like pairs is observed.

  18. Detection and characterization of chemical aerosol using laser-trapping single-particle Raman spectroscopy.

    Science.gov (United States)

    Kalume, Aimable; Beresnev, Leonid A; Santarpia, Joshua; Pan, Yong-Le

    2017-08-10

    Detection and characterization of the presence of chemical agent aerosols in various complex atmospheric environments is an essential defense mission. Raman spectroscopy has the ability to identify chemical molecules, but there are limited numbers of photons detectable from single airborne aerosol particles as they are flowing through a detection system. In this paper, we report on a single-particle Raman spectrometer system that can measure strong spontaneous, stimulated, and resonance Raman spectral peaks from a single laser-trapped chemical aerosol particle, such as a droplet of the VX nerve agent chemical simulant diethyl phthalate. Using this system, time-resolved Raman spectra and elastic scattered intensities were recorded to monitor the chemical properties and size variation of the trapped particle. Such a system supplies a new approach for the detection and characterization of single airborne chemical aerosol particles.

  19. Single particle dynamics and nonlinear resonances in circular accelerators

    International Nuclear Information System (INIS)

    Ruth, R.D.

    1985-11-01

    The purpose of this paper is to introduce the reader to single particle dynamics in circular accelerators with an emphasis on nonlinear resonances. We begin with the Hamiltonian and the equations of motion in the neighborhood of the design orbit. In the linear theory this yields linear betatron oscillations about a closed orbit. It is useful then to introduce the action-angle variables of the linear problem. Next we discuss the nonlinear terms which are present in an actual accelerator, and in particular, we motivate the inclusion of sextupoles to cure chromatic effects. To study the effects of the nonlinear terms, we next discuss canonical perturbation theory which leads us to nonlinear resonances. After showing a few examples of perturbation theory, we abandon it when very close to a resonance. This leads to the study of an isolated resonance in one degree of freedom with a 'time'-dependent Hamiltonian. We see the familiar resonance structure in phase space which is simply closed islands when the nonlinear amplitude dependence of the frequency or 'tune' is included. To show the limits of the validity of the isolated resonance approximation, we discuss two criteria for the onset of chaotic motion. Finally, we study an isolated coupling resonance in two degrees of freedom with a 'time'-dependent Hamiltonian and calculate the two invariants in this case. This leads to a surface of section which is a 2-torus in 4-dimensional phase space. However, we show that it remains a 2-torus when projected into particular 3-dimensional subspaces, and thus can be viewed in perspective

  20. Laboratory Measurements of Single-Particle Polarimetric Spectrum

    Science.gov (United States)

    Gritsevich, M.; Penttila, A.; Maconi, G.; Kassamakov, I.; Helander, P.; Puranen, T.; Salmi, A.; Hæggström, E.; Muinonen, K.

    2017-12-01

    Measuring scattering properties of different targets is important for material characterization, remote sensing applications, and for verifying theoretical results. Furthermore, there are usually simplifications made when we model targets and compute the scattering properties, e.g., ideal shape or constant optical parameters throughout the target material. Experimental studies help in understanding the link between the observed properties and computed results. Experimentally derived Mueller matrices of studied particles can be used as input for larger-scale scattering simulations, e.g., radiative transfer computations. This method allows to bypass the problem of using an idealized model for single-particle optical properties. While existing approaches offer ensemble- and orientation-averaged particle properties, our aim is to measure individual particles with controlled or known orientation. With the newly developed scatterometer, we aim to offer novel possibility to measure single, small (down to μm-scale) targets and their polarimetric spectra. This work presents an experimental setup that measures light scattered by a fixed small particle with dimensions ranging between micrometer and millimeter sizes. The goal of our setup is nondestructive characterization of such particles by measuring light of multiple wavelengths scattered in 360° in a horizontal plane by an ultrasonically levitating sample, whilst simultaneously controlling its 3D position and orientation. We describe the principles and design of our instrument and its calibration. We also present example measurements of real samples. This study was conducted under the support from the European Research Council, in the frame of the Advanced Grant project No. 320773 `Scattering and Absorption of Electromagnetic Waves in Particulate Media' (SAEMPL).

  1. Drift correction of the dissolved signal in single particle ICPMS.

    Science.gov (United States)

    Cornelis, Geert; Rauch, Sebastien

    2016-07-01

    A method is presented where drift, the random fluctuation of the signal intensity, is compensated for based on the estimation of the drift function by a moving average. It was shown using single particle ICPMS (spICPMS) measurements of 10 and 60 nm Au NPs that drift reduces accuracy of spICPMS analysis at the calibration stage and during calculations of the particle size distribution (PSD), but that the present method can again correct the average signal intensity as well as the signal distribution of particle-containing samples skewed by drift. Moreover, deconvolution, a method that models signal distributions of dissolved signals, fails in some cases when using standards and samples affected by drift, but the present method was shown to improve accuracy again. Relatively high particle signals have to be removed prior to drift correction in this procedure, which was done using a 3 × sigma method, and the signals are treated separately and added again. The method can also correct for flicker noise that increases when signal intensity is increased because of drift. The accuracy was improved in many cases when flicker correction was used, but when accurate results were obtained despite drift, the correction procedures did not reduce accuracy. The procedure may be useful to extract results from experimental runs that would otherwise have to be run again. Graphical Abstract A method is presented where a spICP-MS signal affected by drift (left) is corrected (right) by adjusting the local (moving) averages (green) and standard deviations (purple) to the respective values at a reference time (red). In combination with removing particle events (blue) in the case of calibration standards, this method is shown to obtain particle size distributions where that would otherwise be impossible, even when the deconvolution method is used to discriminate dissolved and particle signals.

  2. Crosslinked Functional Polymer Nanowire Formation Along Single Particle Tracks

    International Nuclear Information System (INIS)

    Tagawa, S.

    2006-01-01

    The use of high-energy charged particles has extended to many fields in recent years. In medicine, non-homogeneous energy deposition along an ion trajectory (ion track) plays a crucial role in cancer radiotherapy, allowing for high spatial selectivity in the distribution of the radiation dose. The direct observation and application of ion tracks in media have also attracted interest in materials science, where it is known as nuclear track fabrication. Since the discovery that high-energy particle leave latent tracks in inorganic and organic polymer materials, the technique has also been applied to the production of micro- and nano-sized pores in materials through chemical etching of the tracks. The clear correlation between the etched pore and the characteristics of the incident charged particle has been utilized for measurement of the velocity and mass of the incident particles, and such organic film detectors are widely used in dosimetry, and in particular for galactic cosmic rays in space. The scope of the present paper is the direct nano-structure formation based on crosslinking reactions induced in nano-scale ultra-small spaces of single particle tracks. We have developed the simple one-step formation processes of nanowires without using any chemical etching or refilling processes. The present technique is in striking contrast to the previous 'nuclear track' nanofabrication techniques. According to its high feasibility for the preparation of 1-D nanowires based on 'any' kinds of polymeric materials, the present paper demonstrates the formation of not only simple polymer nanowires but also ceramic and/or multi-segment multi-functional nanowires

  3. DEMONIC programming: a computational language for single-particle equilibrium thermodynamics, and its formal semantics.

    Directory of Open Access Journals (Sweden)

    Samson Abramsky

    2015-11-01

    Full Text Available Maxwell's Demon, 'a being whose faculties are so sharpened that he can follow every molecule in its course', has been the centre of much debate about its abilities to violate the second law of thermodynamics. Landauer's hypothesis, that the Demon must erase its memory and incur a thermodynamic cost, has become the standard response to Maxwell's dilemma, and its implications for the thermodynamics of computation reach into many areas of quantum and classical computing. It remains, however, still a hypothesis. Debate has often centred around simple toy models of a single particle in a box. Despite their simplicity, the ability of these systems to accurately represent thermodynamics (specifically to satisfy the second law and whether or not they display Landauer Erasure, has been a matter of ongoing argument. The recent Norton-Ladyman controversy is one such example. In this paper we introduce a programming language to describe these simple thermodynamic processes, and give a formal operational semantics and program logic as a basis for formal reasoning about thermodynamic systems. We formalise the basic single-particle operations as statements in the language, and then show that the second law must be satisfied by any composition of these basic operations. This is done by finding a computational invariant of the system. We show, furthermore, that this invariant requires an erasure cost to exist within the system, equal to kTln2 for a bit of information: Landauer Erasure becomes a theorem of the formal system. The Norton-Ladyman controversy can therefore be resolved in a rigorous fashion, and moreover the formalism we introduce gives a set of reasoning tools for further analysis of Landauer erasure, which are provably consistent with the second law of thermodynamics.

  4. Synthesis of micro-sized shell-isolated 3D plasmonic superstructures for in situ single-particle SERS monitoring

    Science.gov (United States)

    Zhang, Kun; Zhao, Jingjing; Ji, Ji; Liu, Baohong

    2016-04-01

    A single-particle SERS system enabling real-time and in situ observation of Au-catalyzed reactions has been developed. Both the catalytic activity and the SERS effect are coupled into a single bi-functional 3D superstructure comprising Au nanosatellites self-assembled onto a shell-insulated Ag microflower core, which eliminates the interference from photocatalysis.A single-particle SERS system enabling real-time and in situ observation of Au-catalyzed reactions has been developed. Both the catalytic activity and the SERS effect are coupled into a single bi-functional 3D superstructure comprising Au nanosatellites self-assembled onto a shell-insulated Ag microflower core, which eliminates the interference from photocatalysis. Electronic supplementary information (ESI) available: Details of the synthesis and characterization of the Ag@SiO2@Au superstructures (SEM and TEM images, UV/vis and SERS spectra). See DOI: 10.1039/c6nr00278a

  5. Planck scale physics of the single-particle Schrödinger equation ...

    Indian Academy of Sciences (India)

    August 2002 physics pp. 375–383. Planck scale physics of the single-particle Schrödinger equation with gravitational self-interaction. VIKRAM SONI. National Physical Laboratory, K.S. Krishnan Marg, New Delhi 110 016, India. Abstract. We consider the modification of a single-particle Schrödinger equation by the inclusion.

  6. Effect of acoustic waveguide properties on the Brillouin gain spectrum in multi-mode fibers

    Science.gov (United States)

    Ke, Wei-Wei; Wang, Xiao-Jun; Tang, Xuan

    2015-02-01

    With a recent developed mode-coupling model, the Brillouin gain spectra (BGS) of multi-mode fibers (MMF) are investigated and compared with the corresponding experiment. It is found that the calculation results are coincident well with the experiment data. Furthermore, the BGS are found to be very sensitive to the index fluctuation. Such phenomenon is demonstrated by introducing a small index hump or dip in the center of the fiber core. And it can be explained by that the index fluctuation may influence the acoustic mode greatly.

  7. Improving the theoretical foundations of the multi-mode transport model

    International Nuclear Information System (INIS)

    Bateman, G.; Kritz, A.H.; Redd, A.J.; Erba, M.; Rewoldt, G.; Weiland, J.; Strand, P.; Kinsey, J.E.; Scott, B.

    2001-01-01

    A new version of the Multi-Mode transport model, designated MMM98, is being developed with improved theoretical foundations, in an ongoing effort to predict the temperature and density profiles in tokamaks. For transport near the edge of the plasma, MMM98 uses a new model based on 3-D nonlinear simulations of drift Alfven mode turbulence. Flow shear stabilization effects have been added to the Weiland model for Ion Temperature Gradient and Trapped Electron Modes, which usually dominates in most of the plasma core. For transport near the magnetic axis at high beta, a new kinetic ballooning mode model has been constructed based on FULL stability code computations. (author)

  8. Heralded source of bright multi-mode mesoscopic sub-Poissonian light

    DEFF Research Database (Denmark)

    Iskhakov, Timur; Usenko, V. C.; Andersen, Ulrik Lund

    2016-01-01

    In a direct detection scheme, we observed 7.8 dB of twin-beam squeezing for multi-mode two-color squeezed vacuum generated via parametric downconversion. Applying postselection, we conditionally prepared a sub-Poissonian state of light containing 6.3 . 105 photons per pulse on the average...... with the Fano factor 0.63 +/- 0.01. The scheme can be considered as the heralded preparation of pulses with the mean energy varying between tens and hundreds of fJ and the uncertainty considerably below the shot-noise level. Such pulses can be used in metrology (for instance, for radiometer calibration...

  9. Multi-mode to single-mode conversion in a 61 port photonic lantern

    DEFF Research Database (Denmark)

    Noordegraaf, Danny; Skovgaard, Peter M.W.; Maack, Martin D.

    2010-01-01

    Efficient multi-mode (MM) to single-mode (SM) conversion in a 61 port splitter or “Photonic Lantern” is demonstrated. The coupling loss from a 100 µm core diameter MM section to an ensemble of 61 SM fibers and back to another 100 µm core MM section is measured to be as low as 0.76 dB. This demons......B. This demonstration shows the feasibility of using the Photonic Lanterns within the field of astrophotonics for coupling MM star-light to an ensemble of SM fibers in order to perform fiber Bragg grating based spectral filtering."...

  10. Chaos and nonlinear dynamics of single-particle orbits in a magnetotaillike magnetic field

    Science.gov (United States)

    Chen, J.; Palmadesso, P. J.

    1986-01-01

    The properties of charged-particle motion in Hamiltonian dynamics are studied in a magnetotaillike magnetic field configuration. It is shown by numerical integration of the equation of motion that the system is generally nonintegrable and that the particle motion can be classified into three distinct types of orbits: bounded integrable orbits, unbounded stochastic orbits, and unbounded transient orbits. It is also shown that different regions of the phase space exhibit qualitatively different responses to external influences. The concept of 'differential memory' in single-particle distributions is proposed. Physical implications for the dynamical properties of the magnetotail plasmas and the possible generation of non-Maxwellian features in the distribution functions are discussed.

  11. A Multi-Mode Blade Damping Control using Shunted Piezoelectric Transducers with Active Feedback Structure

    Science.gov (United States)

    Choi, Benjamin; Morrison, Carlos; Min, James

    2009-01-01

    The Structural Dynamics and. Mechanics branch (RXS) is developing smart adaptive structures to improve fan blade damping at resonances using piezoelectric (PE) transducers. In this presentation, only one shunted PE transducer was used to demonstrate active control of multi-mode blade resonance damping on a titanium alloy (Ti-6A1-4V) flat plate model, regardless of bending, torsion, and 2-stripe modes. This work would have a significant impact on the conventional passive shunt damping world because the standard feedback control design tools can now be used to design and implement electric shunt for vibration control. In other words, the passive shunt circuit components using massive inductors and. resistors for multi-mode resonance control can be replaced with digital codes. Furthermore, this active approach with multi patches can simultaneously control several modes in the engine operating range. Dr. Benjamin Choi presented the analytical and experimental results from this work at the Propulsion-Safety and. Affordable Readiness (P-SAR) Conference in March, 2009.

  12. Single Particle Soot Photometer intercomparison at the AIDA chamber

    Directory of Open Access Journals (Sweden)

    M. Laborde

    2012-12-01

    Full Text Available Soot particles, consisting of black carbon (BC, organic carbon (OC, inorganic salts, and trace elements, are emitted into the atmosphere during incomplete combustion. Accurate measurements of atmospheric BC are important as BC particles cause adverse health effects and impact the climate.

    Unfortunately, the accurate measurement of the properties and mass concentrations of BC particles remains difficult. The Single Particle Soot Photometer (SP2 can contribute to improving this situation by measuring the mass of refractory BC in individual particles as well as its mixing state.

    Here, the results of the first detailed SP2 intercomparison, involving 6 SP2s from 6 different research groups, are presented, including the most evolved data products that can presently be calculated from SP2 measurements.

    It was shown that a detection efficiency of almost 100% down to 1 fg BC per particle can readily be achieved, and that this limit can be pushed down to ∼0.2 fg BC with optimal SP2 setup. Number and mass size distributions of BC cores agreed within ±5% and ±10%, respectively, in between the SP2s, with larger deviations in the range below 1 fg BC.

    The accuracy of the SP2's mass concentration measurement depends on the calibration material chosen. The SP2 has previously been shown to be equally sensitive to fullerene soot and ambient BC from sources where fossil fuel was dominant and less sensitive to fullerene soot than to Aquadag. Fullerene soot was therefore chosen as the standard calibration material by the SP2 user community; however, many data sets rely solely on Aquadag calibration measurements. The difference in SP2 sensitivity was found to be almost equal (fullerene soot to Aquadag response ratio of ∼0.75 at 8.9 fg BC for all SP2s. This allows the calculation of a fullerene soot equivalent calibration curve from a measured Aquadag calibration, when no fullerene soot calibration is available. It could be

  13. Optical, magnetic, and single-particle excitations in the multiband Hubbard model for cuprate superconductors

    Science.gov (United States)

    Wagner, J.; Hanke, W.; Scalapino, D. J.

    1991-05-01

    On the basis of exact diagonalizations, a comparative study of two-particle optical and magnetic, as well as single-particle, excitations is presented for a two-dimensional (2D) multiorbital Hubbard model. For reasonable parameter sets appropriate for the cuprate superconductors, the single-particle excitations display strongly correlated states related to the Zhang-Rice Cu-O singlet construction. These states define the gap (to the upper Hubbard band) at half-filling and become partially occupied by doping holes in our 2×2 unit-cell system. The optical results, which are the first quantitative calculations performed for realistic parameters of the three-band Hubbard model, clearly show three allowed optical transitions: (i) itinerant motion of the Cu-O singlets, having (for doping concentrations x≠0) a spectral Drude distribution around ω=0 with spectral weight proportional to x; (ii) unbinding of the O hole from the Cu spin in the singlet. This gives, in particular, a strong absorption peak due to singlet-->nonbonding oxygen transitions, again with relative weight ~x. It is roughly centered at ω~JKondoUpd. They show a pronounced excitonic effect due to the p-d interaction Upd and have a reduced spectral weight shifted to higher energies for increased dopings. Findings (i)-(iii) are in general accordance with recent experimental data. Our study of the low-energy absorption is complemented with a numerical scaling analysis of the Drude weight in 1D, where, in particular, we find an interesting violation of Lenz's law for 4n-site Hubbard rings. Finally, the magnetic structure factor is calculated for the 2D case. For finite doping it contains a peak at 2JKondo, which should be detectable in experiment.

  14. Many-particle nucleon-nucleon forces from nuclear single-particle states

    OpenAIRE

    Birbrair, B. L.; Ryazanov, V. I.

    1999-01-01

    As follows from the energies of single-particle states in ^{40}Ca, ^{90}Zr and ^{208}Pb nuclei the contribution of many-particle NN forces to the nuclear single-particle potential is at least the sum of repulsive and attractive parts resulting from three-particle and four-particle forces respectively. In addition the specified nucleon density distributions in the above nuclei are determined from both the 1 GeV proton-nucleus elastic scattering and the single-particle energies.

  15. Multi-Mode Estimation for Small Fixed Wing Unmanned Aerial Vehicle Localization Based on a Linear Matrix Inequality Approach.

    Science.gov (United States)

    Elzoghby, Mostafa; Li, Fu; Arafa, Ibrahim I; Arif, Usman

    2017-04-18

    Information fusion from multiple sensors ensures the accuracy and robustness of a navigation system, especially in the absence of global positioning system (GPS) data which gets degraded in many cases. A way to deal with multi-mode estimation for a small fixed wing unmanned aerial vehicle (UAV) localization framework is proposed, which depends on utilizing a Luenberger observer-based linear matrix inequality (LMI) approach. The proposed estimation technique relies on the interaction between multiple measurement modes and a continuous observer. The state estimation is performed in a switching environment between multiple active sensors to exploit the available information as much as possible, especially in GPS-denied environments. Luenberger observer-based projection is implemented as a continuous observer to optimize the estimation performance. The observer gain might be chosen by solving a Lyapunov equation by means of a LMI algorithm. Convergence is achieved by utilizing the linear matrix inequality (LMI), based on Lyapunov stability which keeps the dynamic estimation error bounded by selecting the observer gain matrix (L). Simulation results are presented for a small UAV fixed wing localization problem. The results obtained using the proposed approach are compared with a single mode Extended Kalman Filter (EKF). Simulation results are presented to demonstrate the viability of the proposed strategy.

  16. A Study of the Multi-Mode Purcell Effect for a Transmon in 3D Circuit QED

    Science.gov (United States)

    Petrenko, Andrei; Sun, Luyan; Blumoff, Jacob; Nigg, Simon; Girvin, Steve; Schoelkopf, Robert

    2013-03-01

    Although superconducting 3D transmon qubits offer a promising path toward realizing an architecture for quantum computation, they are still limited by decoherence processes that are not yet fully understood. Qubit T1 relaxation due to the Purcell Effect presents one such limitation on coherence times, but thus far a complete model of Purcell processes for transmons in 3D cavities, beyond the approximation of a single cavity mode and lumped element qubit, has been absent. Employing a simple scheme to vary the decay rate κ (or quality factor Q) of our cavities in-situ we explore in detail how multiple cavity modes contribute to qubit T1 decay in the Purcell regime. In addition, we show the continued dependence of qubit T1 on cavity κ as we systematically decouple from our cavity and are no longer Purcell-limited and how this dependence is related to a steady rise in qubit excited state population. Our findings are consistent with theory we have developed based on an effective circuit model for the cavity-qubit system, and set the stage for continuing the study of the multi-mode Purcell Effect by means of in-situ tuning of not just the cavity coupling, but the qubit frequency itself.

  17. Multi-Objective Optimization Considering Battery Degradation for a Multi-Mode Power-Split Electric Vehicle

    Directory of Open Access Journals (Sweden)

    Xuerui Ma

    2017-07-01

    Full Text Available A multi-mode power-split (MMPS hybrid electric vehicle (HEV has two planetary gearsets and clutches/grounds which results in several operation modes with enhanced electric drive capability and better fuel economy. Basically, the battery storage system is involved in different operation modes to satisfy the power demand and minimize the fuel consumption, whereas the complicated operation modes with frequent charging/discharging will absolutely influence the battery life because of degradation. In this paper, firstly, we introduce the solid electrolyte interface (SEI film growth model based on the previous study of the battery degradation principles and was verified according to the test data. We consider both the fuel economy and battery degradation as a multi-objective problem for MMPS HEV by normalization with a weighting factor. An instantaneous optimization is implemented based on the equivalent fuel consumption concept. Then the control strategy is implemented on a simulation framework integrating the MMPS powertrain model and the SEI film growth map model over some typical driving cycles, such as New European Driving Cycle (NEDC and Urban Dynamometer Driving Schedule (UDDS. Finally, the result demonstrates that these two objectives are conflicting and the trade-off reduces the battery degradation with fuel sacrifice. Additionally, the analysis reveals how the mode selection will reflect the battery degradation.

  18. Multi-Mode Lamb Wave Arrival Time Extraction for Improved Tomographic Reconstruction

    International Nuclear Information System (INIS)

    Hinders, Mark K.; Hou Jidong; Leonard, Kevin R.

    2005-01-01

    An ultrasonic signal processing technique is applied to multi-mode arrival time estimation from Lamb waveforms. The basic tool is a simplified time-scale projection called a dynamic wavelet fingerprint (DWFP) which enables direct observation of the variation of features of interest in non-stationary ultrasonic signals. The DWFP technique was used to automatically detect and evaluate each candidate through-transmitted Lamb mode. The area of the dynamic wavelet fingerprint was then used as a feature to distinguish false modes caused by noise and other interference from the true modes of interest. The set of estimated arrival times were then used as inputs for tomographic reconstruction. The Lamb wave tomography images generated with these estimated arrival times were able to indicate different defects in aluminum plates

  19. The Combinatorial Multi-Mode Resource Constrained Multi-Project Scheduling Problem

    Directory of Open Access Journals (Sweden)

    Denis Pinha

    2016-11-01

    Full Text Available This paper presents the formulation and solution of the Combinatorial Multi-Mode Resource Constrained Multi-Project Scheduling Problem. The focus of the proposed method is not on finding a single optimal solution, instead on presenting multiple feasible solutions, with cost and duration information to the project manager. The motivation for developing such an approach is due in part to practical situations where the definition of optimal changes on a regular basis. The proposed approach empowers the project manager to determine what is optimal, on a given day, under the current constraints, such as, change of priorities, lack of skilled worker. The proposed method utilizes a simulation approach to determine feasible solutions, under the current constraints. Resources can be non-consumable, consumable, or doubly constrained. The paper also presents a real-life case study dealing with scheduling of ship repair activities.

  20. Time displacement pictures with multi-mode probes from circumferential welds

    International Nuclear Information System (INIS)

    Wustenberg, H.; Jaffrey, D.; Ludwig, B.; Bertus, N.; Erhard, A.

    1985-01-01

    If a creeping wave probe is applied to butt welds typical echo patterns from weld defects can be received. It seems possible that echoes from the geometric shape of the root or the crown and defect echoes can be separated by simple means. This has been the reason for the development of a special presentation of the echo patterns received by this multi-mode creeping wave probe. The so called time displacement pictures show the AD-converted A-scans in a gray scale along a line corresponding to the time axis of the propagation. Perpendicular to this time axis results obtained from displacement of the probe parallel to the weld are presented. This kind of picture immediately provides the whole A-scan information. This paper presents some first results on simulated welds with artificial defects and on circumferential welds with typical geometric imperfections

  1. Refractive index sensors based on the fused tapered special multi-mode fiber

    Science.gov (United States)

    Fu, Xing-hu; Xiu, Yan-li; Liu, Qin; Xie, Hai-yang; Yang, Chuan-qing; Zhang, Shun-yang; Fu, Guang-wei; Bi, Wei-hong

    2016-01-01

    In this paper, a novel refractive index (RI) sensor is proposed based on the fused tapered special multi-mode fiber (SMMF). Firstly, a section of SMMF is spliced between two single-mode fibers (SMFs). Then, the SMMF is processed by a fused tapering machine, and a tapered fiber structure is fabricated. Finally, a fused tapered SMMF sensor is obtained for measuring external RI. The RI sensing mechanism of tapered SMMF sensor is analyzed in detail. For different fused tapering lengths, the experimental results show that the RI sensitivity can be up to 444.517 81 nm/RIU in the RI range of 1.334 9—1.347 0. The RI sensitivity is increased with the increase of fused tapering length. Moreover, it has many advantages, including high sensitivity, compact structure, fast response and wide application range. So it can be used to measure the solution concentration in the fields of biochemistry, health care and food processing.

  2. Contribution to the development of a multi-mode measurement system for dynamic neutronic measurements and processing of the related uncertainties; Contribution au developpement d'un systeme de mesure multimode pour des mesures neutroniques dynamiques et traitement des incertitudes associees

    Energy Technology Data Exchange (ETDEWEB)

    Geslot, B

    2006-11-15

    It is difficult to estimate integral reactor parameters, especially reactivity, in deeply subcritical cores. Indeed the standard neutronic methods have been designed for near critical reactivity levels and they often need a critical reference. This thesis takes part in the research on ADS (Accelerated Driven Systems), for which the multiplication coefficient would be about 0.95. The first part of the thesis deals with the development of the XMODE system. It is a flexible measurement system dedicated to experiments in neutronics. X-MODE is capable of acquiring logical signals particularly in time-stamping mode as well as analogical signals. The second part of the thesis presents a statistical study of the methods used to analyse flux transients. Indeed a lot of methods exist to analyse flux transients and some are little known. Means to estimate characteristics of reactivity estimators are provided, methods compared and recommendations made. Finally, the dynamic measurements of the TRADE program are analysed and discussed. During this program, three subcritical configurations were explored. It appears that pulsed neutron source experiments give reactivity estimations that are much more precise than those obtained from flux transients. (author)

  3. Mass Spectrometry of Single Particles Levitated in an Electrodynamic Balance: Applications to Laboratory Atmospheric Chemistry Research

    Science.gov (United States)

    Birdsall, A.; Krieger, U. K.; Keutsch, F. N.

    2017-12-01

    Dynamic changes to atmospheric aerosol particle composition (e.g., originating from evaporation/condensation, oxidative aging, or aqueous-phase chemical reactions) impact particle properties with importance for understanding particle effects on climate and human health. These changes can take place over the entire lifetime of an atmospheric particle, which can extend over multiple days. Previous laboratory studies of such processes have included analyzing single particles suspended in a levitation device, such as an electrodynamic balance (EDB), an optical levitator, or an acoustic trap, using optical detection techniques. However, studying chemically complex systems can require an analytical method, such as mass spectrometry, that provides more molecular specificity. Existing work coupling particle levitation with mass spectrometry is more limited and largely has consisted of acoustic levitation of millimeter-sized droplets.In this work an EDB has been coupled with a custom-built ionization source and commercial time-of-flight mass spectrometer (MS) as a platform for laboratory atmospheric chemistry research. Single charged particles (radius 10 μm) have been injected into an EDB, levitated for an arbitrarily long period of time, and then transferred to a vaporization-corona discharge ionization region for MS analysis. By analyzing a series of particles of identical composition, residing in the controlled environment of the EDB for varying times, we can trace the chemical evolution of a particle over hours or days, appropriate timescales for understanding transformations of atmospheric particles.To prove the concept of our EDB-MS system, we have studied the evaporation of particles consisting of polyethylene glycol (PEG) molecules of mixed chain lengths, used as a benchmark system. Our system can quantify the composition of single particles (see Figure for sample spectrum of a single PEG-200 particle: PEG parent ions labeled with m/z, known PEG fragment ions

  4. Pick-off annihilation of positronium in matter using full correlation single particle potentials: solid He.

    Science.gov (United States)

    Zubiaga, A; Tuomisto, F; Puska, M J

    2015-01-29

    We investigate the modeling of positronium (Ps) states and their pick-off annihilation trapped at open volumes pockets in condensed molecular matter. Our starting point is the interacting many-body system of Ps and a He atom because it is the smallest entity that can mimic the energy gap between the highest occupied and lowest unoccupied molecular orbitals of molecules, and yet the many-body structure of the HePs system can be calculated accurately enough. The exact-diagonalization solution of the HePs system enables us to construct a pairwise full-correlation single-particle potential for the Ps-He interaction, and the total potential in solids is obtained as a superposition of the pairwise potentials. We study in detail Ps states and their pick-off annihilation rates in voids inside solid He and analyze experimental results for Ps-induced voids in liquid He obtaining the radii of the voids. More importantly, we generalize our conclusions by testing the validity of the Tao-Eldrup model, widely used to analyze ortho-Ps annihilation measurements for voids in molecular matter, against our theoretical results for the solid He. Moreover, we discuss the influence of the partial charges of polar molecules and the strength of the van der Waals interaction on the pick-off annihilation rate.

  5. Characterizing physical properties and heterogeneous chemistry of single particles in air using optical trapping-Raman spectroscopy

    Science.gov (United States)

    Gong, Z.; Wang, C.; Pan, Y. L.; Videen, G.

    2017-12-01

    Heterogeneous reactions of solid particles in a gaseous environment are of increasing interest; however, most of the heterogeneous chemistry studies of airborne solids were conducted on particle ensembles. A close examination on the heterogeneous chemistry between single particles and gaseous-environment species is the key to elucidate the fundamental mechanisms of hydroscopic growth, cloud nuclei condensation, secondary aerosol formation, etc., and reduce the uncertainty of models in radiative forcing, climate change, and atmospheric chemistry. We demonstrate an optical trapping-Raman spectroscopy (OT-RS) system to study the heterogeneous chemistry of the solid particles in air at single-particle level. Compared to other single-particle techniques, optical trapping offers a non-invasive, flexible, and stable method to isolate single solid particle from substrates. Benefited from two counter-propagating hollow beams, the optical trapping configuration is adaptive to trap a variety of particles with different materials from inorganic substitution (carbon nanotubes, silica, etc.) to organic, dye-doped polymers and bioaerosols (spores, pollen, etc.), with different optical properties from transparent to strongly absorbing, with different sizes from sub-micrometers to tens of microns, or with distinct morphologies from loosely packed nanotubes to microspheres and irregular pollen grains. The particles in the optical trap may stay unchanged, surface degraded, or optically fragmented according to different laser intensity, and their physical and chemical properties are characterized by the Raman spectra and imaging system simultaneously. The Raman spectra is able to distinguish the chemical compositions of different particles, while the synchronized imaging system can resolve their physical properties (sizes, shapes, morphologies, etc.). The temporal behavior of the trapped particles also can be monitored by the OT-RS system at an indefinite time with a resolution from

  6. Tuning the dispersion and single/multi-modeness of a hole-assisted fiber by the hole's geometrical parameters

    NARCIS (Netherlands)

    Uranus, H.P.; Hoekstra, Hugo; van Groesen, Embrecht W.C.

    2008-01-01

    Using a vectorial finite element mode solver developed earlier, we studied a hole-assisted multi-ring fiber. We report the role of the hole’s geometrical parameters in tuning the waveguide dispersion and the single/multi-modeness of the particular fiber. By correctly selecting the hole’s size and

  7. Reconfigurable Bandpass Sigma-Delta Modulator With Programmable NTF for Low-IF Multi-Mode Receivers

    DEFF Research Database (Denmark)

    Zhang, Ke; Mikkelsen, Jan H.; Shen, Ming

    2012-01-01

    transfer function of the loop while still maintaining stability. Compared with conventional multi-mode BPSDM, employing cascade structures and multi-bit sub-ADCs, the proposed modulator features many attractive advantages, such as (1) avoiding coefficient mismatch between analog and digital components...

  8. A review of progress in single particle tracking: from methods to biophysical insights

    Science.gov (United States)

    Manzo, Carlo; Garcia-Parajo, Maria F.

    2015-12-01

    Optical microscopy has for centuries been a key tool to study living cells with minimum invasiveness. The advent of single molecule techniques over the past two decades has revolutionized the field of cell biology by providing a more quantitative picture of the complex and highly dynamic organization of living systems. Amongst these techniques, single particle tracking (SPT) has emerged as a powerful approach to study a variety of dynamic processes in life sciences. SPT provides access to single molecule behavior in the natural context of living cells, thereby allowing a complete statistical characterization of the system under study. In this review we describe the foundations of SPT together with novel optical implementations that nowadays allow the investigation of single molecule dynamic events with increasingly high spatiotemporal resolution using molecular densities closer to physiological expression levels. We outline some of the algorithms for the faithful reconstruction of SPT trajectories as well as data analysis, and highlight biological examples where the technique has provided novel insights into the role of diffusion regulating cellular function. The last part of the review concentrates on different theoretical models that describe anomalous transport behavior and ergodicity breaking observed from SPT studies in living cells.

  9. Fluorescence preselection of bioaerosol for single-particle mass spectrometry

    NARCIS (Netherlands)

    Stowers, M.A.; Van Wuijckhuijse, A.L.; Marijnissen, J.C.M.; Kientz, C.E.; Ciach, T.

    2006-01-01

    We have designed, constructed, and tested a system that preselects the biological fraction of airborne particles from the overall aerosol. The preselection is based on fluorescence emission excited by a continuous 266 nm laser beam. This beam is one of two cw beams used to measure the aerodynamic

  10. Online single particle measurements of black carbon coatings, structure and optical properties

    Science.gov (United States)

    Allan, James; Liu, Dantong; Taylor, Jonathan; Flynn, Michael; Williams, Paul; Morgan, William; Whitehead, James; Alfarra, Rami; McFiggans, Gordon; Coe, Hugh

    2016-04-01

    The impacts of black carbon on meteorology and climate remain a major source of uncertainty, owing in part to the complex relationship between the bulk composition of the particulates and their optical properties. A particular complication stems from how light interacts with particles in response to the microphysical configuration and any 'coatings', i.e. non-black carbon material that is either co-emitted or subsequently obtained through atmospheric processing. This may cause the particle to more efficiently absorb or scatter light and may even change the sign of its radiative forcing potential. While much insight has been gained through measurements of bulk aerosol properties, either while suspended or after collection on a filter or impactor substrate, this does not provide a complete picture and thus may not adequately constrain the system. Here we present an overview of recent work to better constrain the properties of black carbon using online, in situ measurements of single particles, primarily using a Single Particle Soot Photometer (SP2). We have developed novel methods of inverting the data produced and combining the different metrics derived so as to give the most effective insights into black carbon sources, processes and properties. We have also used this measurement in conjunction with other instruments (sometimes in series) and used the data to challenge many commonly used models of optical properties such as core-shell Mie, Rayleigh-Debeye-Gans and effective medium. This work has been carried out in a variety of atmospheric environments and with laboratory-produced soots, e.g. from a diesel engine rig. Highlights include the finding that with real-world atmospheric aerosols, bulk optical measurements may be insufficient to derive brown carbon parameters without detailed morphological data. We also show that the enhancement of absorption for both ambient and laboratory generated particles only occurs after the coating mass fraction reaches a certain

  11. Theoretical investigations of single particle spectroscopies of novel materials

    Energy Technology Data Exchange (ETDEWEB)

    Randeria, Mohit [The Ohio State Univ., Columbus, OH (United States)

    2017-02-24

    The project focused on three areas in the theoretical investigation of quantum materials. The first was novel magnetism in low dimensional systems, especially chiral magnetism and topological spin textures that can arise in thin films and at interfaces. The second related to high temperature superconductivity, and particularly on understanding puzzling features of quantum oscillations. The third related to collaborations with experimentalists on angle-resolved photoemission spectroscopy of high Tc superconductors and charge density wave materials.

  12. Single particle mass spectral signatures from vehicle exhaust particles and the source apportionment of on-line PM2.5by single particle aerosol mass spectrometry.

    Science.gov (United States)

    Yang, Jian; Ma, Shexia; Gao, Bo; Li, Xiaoying; Zhang, Yanjun; Cai, Jing; Li, Mei; Yao, Ling'ai; Huang, Bo; Zheng, Mei

    2017-09-01

    In order to accurately apportion the many distinct types of individual particles observed, it is necessary to characterize fingerprints of individual particles emitted directly from known sources. In this study, single particle mass spectral signatures from vehicle exhaust particles in a tunnel were performed. These data were used to evaluate particle signatures in a real-world PM 2.5 apportionment study. The dominant chemical type originating from average positive and negative mass spectra for vehicle exhaust particles are EC species. Four distinct particle types describe the majority of particles emitted by vehicle exhaust particles in this tunnel. Each particle class is labeled according to the most significant chemical features in both average positive and negative mass spectral signatures, including ECOC, NaK, Metal and PAHs species. A single particle aerosol mass spectrometry (SPAMS) was also employed during the winter of 2013 in Guangzhou to determine both the size and chemical composition of individual atmospheric particles, with vacuum aerodynamic diameter (d va ) in the size range of 0.2-2μm. A total of 487,570 particles were chemically analyzed with positive and negative ion mass spectra and a large set of single particle mass spectra was collected and analyzed in order to identify the speciation. According to the typical tracer ions from different source types and classification by the ART-2a algorithm which uses source fingerprints for apportioning ambient particles, the major sources of single particles were simulated. Coal combustion, vehicle exhaust, and secondary ion were the most abundant particle sources, contributing 28.5%, 17.8%, and 18.2%, respectively. The fraction with vehicle exhaust species particles decreased slightly with particle size in the condensation mode particles. Copyright © 2017 Elsevier B.V. All rights reserved.

  13. cisTEM, user-friendly software for single-particle image processing.

    Science.gov (United States)

    Grant, Timothy; Rohou, Alexis; Grigorieff, Nikolaus

    2018-03-07

    We have developed new open-source software called cis TEM (computational imaging system for transmission electron microscopy) for the processing of data for high-resolution electron cryo-microscopy and single-particle averaging. cis TEM features a graphical user interface that is used to submit jobs, monitor their progress, and display results. It implements a full processing pipeline including movie processing, image defocus determination, automatic particle picking, 2D classification, ab-initio 3D map generation from random parameters, 3D classification, and high-resolution refinement and reconstruction. Some of these steps implement newly-developed algorithms; others were adapted from previously published algorithms. The software is optimized to enable processing of typical datasets (2000 micrographs, 200 k - 300 k particles) on a high-end, CPU-based workstation in half a day or less, comparable to GPU-accelerated processing. Jobs can also be scheduled on large computer clusters using flexible run profiles that can be adapted for most computing environments. cis TEM is available for download from cistem.org. © 2018, Grant et al.

  14. Accelerators for critical experiments involving single-particle upset in solid-state microcircuits

    Science.gov (United States)

    Zoutendyk, J. A.

    1985-01-01

    Charged-particle interactions in microelectronic circuit chips (integrated circuits) present a particularly insidious problem for solid-state electronic systems due to the generation of soft errors or single-particle event upset (SEU) by either cosmic rays or other radiation sources. Particle accelerators are used to provide both light and heavy ions in order to assess the propensity of integrated circuit chips for SEU. Critical aspects of this assessment involve the ability to analytically model SEU for the prediction of error rates in known radiation environments. In order to accurately model SEU, the measurement and prediction of energy deposition in the form of an electron-hole plasma generated along an ion track is of paramount importance. This requires the use of accelerators which allow for ease in both energy control (change of energy) and change of ion species. This and other aspects of ion-beam control and diagnostics (e.g., uniformity and flux) are of critical concern for the experimental verification of theoretical SEU models.

  15. Scattering measurement of single particle for highly sensitive homogeneous detection of DNA in serum.

    Science.gov (United States)

    Zhu, Liang; Li, Guohua; He, Yonghong; Tan, Hui; Sun, Shuqing

    2018-02-01

    A highly sensitive homogeneous method for DNA detection has been developed. The system relies on two kinds of gold nanorod (AuNR) probes with complementary DNA sequences to the target DNA. In the presence of the target DNA, two kinds of AuNR probes are assembling into dimers or small aggregates. The target-induced AuNR aggregate has higher scattering intensity than that of a single AuNR because of the plasmonic coupling effect. Dark field microscopy was utilized to image the single particle and measure its scattering intensity. We wrote our own Matlab code and used it to extract the scattering signal of all particles. Difference in distribution of scattering intensity between the single AuNR and its aggregate provides a quantitative basis for the detection of target DNA. A linear dynamic range spanning from 0.1pM to 1nM and a detection limit of ~ 30fM were achieved for the detection of DNA in serum sample. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. Single particle nonlocality, geometric phases and time-dependent boundary conditions

    Science.gov (United States)

    Matzkin, A.

    2018-03-01

    We investigate the issue of single particle nonlocality in a quantum system subjected to time-dependent boundary conditions. We discuss earlier claims according to which the quantum state of a particle remaining localized at the center of an infinite well with moving walls would be specifically modified by the change in boundary conditions due to the wall’s motion. We first prove that the evolution of an initially localized Gaussian state is not affected nonlocally by a linearly moving wall: as long as the quantum state has negligible amplitude near the wall, the boundary motion has no effect. This result is further extended to related confined time-dependent oscillators in which the boundary’s motion is known to give rise to geometric phases: for a Gaussian state remaining localized far from the boundaries, the effect of the geometric phases is washed out and the particle dynamics shows no traces of a nonlocal influence that would be induced by the moving boundaries.

  17. Surface chemistry and morphology in single particle optical imaging

    Science.gov (United States)

    Ekiz-Kanik, Fulya; Sevenler, Derin Deniz; Ünlü, Neşe Lortlar; Chiari, Marcella; Ünlü, M. Selim

    2017-05-01

    Biological nanoparticles such as viruses and exosomes are important biomarkers for a range of medical conditions, from infectious diseases to cancer. Biological sensors that detect whole viruses and exosomes with high specificity, yet without additional labeling, are promising because they reduce the complexity of sample preparation and may improve measurement quality by retaining information about nanoscale physical structure of the bio-nanoparticle (BNP). Towards this end, a variety of BNP biosensor technologies have been developed, several of which are capable of enumerating the precise number of detected viruses or exosomes and analyzing physical properties of each individual particle. Optical imaging techniques are promising candidates among broad range of label-free nanoparticle detectors. These imaging BNP sensors detect the binding of single nanoparticles on a flat surface functionalized with a specific capture molecule or an array of multiplexed capture probes. The functionalization step confers all molecular specificity for the sensor's target but can introduce an unforeseen problem; a rough and inhomogeneous surface coating can be a source of noise, as these sensors detect small local changes in optical refractive index. In this paper, we review several optical technologies for label-free BNP detectors with a focus on imaging systems. We compare the surface-imaging methods including dark-field, surface plasmon resonance imaging and interference reflectance imaging. We discuss the importance of ensuring consistently uniform and smooth surface coatings of capture molecules for these types of biosensors and finally summarize several methods that have been developed towards addressing this challenge.

  18. Experimental Investigation of Multi-mode Fiber Laser Cutting of Cement Mortar

    Science.gov (United States)

    2018-01-01

    This study successfully applied multi-mode laser cutting with the variation of the laser cutting speed to cement mortar for the first time. The effects of the amount of silica sand in the cement mortar on laser cutting are tested and analyzed. The kerf width and penetration depth of the specimens after laser cutting are investigated. As the laser cutting speed increases, the penetration depth decreases for both cement paste and cement mortar, whereas the kerf width becomes saturated and increases, respectively, for cement paste and cement mortar. Cross sections of the specimens are compared with illustrations. Top-view images of the cement mortar with indicators of the physical characteristics, such as re-solidification, burning, and cracks are examined, and the possible causes of these characteristics are explained. The optical absorption rates of cement-based materials are quantified at wide ranges of wavelength to compare the absorption rates in accordance with the materials compositions. The chemical composition variation before and after laser cutting is also compared by EDX (Energy Dispersive X-Ray) analysis. In addition to these observations, material removal mechanisms for cement mortar are proposed. PMID:29439431

  19. The multi-mode modulator: A versatile fluidic device for two-dimensional gas chromatography.

    Science.gov (United States)

    Seeley, John V; Schimmel, Nicolaas E; Seeley, Stacy K

    2018-02-09

    A fluidic device called the multi-mode modulator (MMM) has been developed for use as a comprehensive two-dimensional gas chromatography (GC x GC) modulator. The MMM can be employed in a wide range of capacities including as a traditional heart-cutting device, a low duty cycle GC x GC modulator, and a full transfer GC x GC modulator. The MMM is capable of producing narrow component pulses (widths modulated components is confined to the interior of a joining capillary. The joining capillary dimensions and the position of the columns within the joining capillary can be optimized for the selected modulation mode. Furthermore, the joining capillary can be replaced easily and inexpensively if it becomes fouled due to sample matrix components or column bleed. The principles of operation of the MMM are described and its efficacy is demonstrated as a heart-cutting device and as a GC x GC modulator. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Multi-mode dynamics of optical oscillators based on intracavity nonlinear frequency down-conversion

    Science.gov (United States)

    Morozov, Yuri A.

    2018-01-01

    The transient power characteristics of two optical oscillators—a difference frequency generator (ICDFG) and a singly resonant optical parametric oscillator (ICSRO)—based on intracavity nonlinear optical frequency conversion, are described. The simulation has been performed via the rate-equation mathematical model, which features a multi-mode behavior of all optical fields. The reason for unattainability of single-mode emission in these devices without an additional frequency-selective element (e.g., a Fabry-Perot etalon) is clarified. It is shown that the dynamics of a short-wavelength emission (pump) results mainly from the nonlinear optical interaction, while that of the longer-wavelength optical fields (signal and idler) depends on selectivity of the etalon. With the suitable etalons inserted in their cavities, both devices are shown to operate dynamically single-mode under conventional experimental conditions. The nonlinear interaction makes the pump emission collapse to the single-mode operation very fast (it takes no more than a few tens of the photon lifetimes). To overcome the threshold of parametric generation, the intracavity pump power in the ICSRO has to exceed ˜ 100 W, while the ICDFG is essentially a "thresholdless" device.

  1. Optimizing strategy for repetitive construction projects within multi-mode resources

    Directory of Open Access Journals (Sweden)

    Remon Fayek Aziz

    2013-03-01

    Full Text Available Estimating tender data for specific project is the most essential part in construction areas as of a contractor’s view such as: proposed project duration with corresponding gross value and cash flows. Cash flow analysis of construction projects has a long history and has been an important topic in construction management. Determination of project cash flows is very sensitive, especially for repetitive construction projects. This paper focuses on how to calculate tender data for repetitive construction projects such as: project duration, project cost, project/bid price, project cash flows, project maximum working capital and project net present value that is equivalent to net profit at the beginning of the project. A simplified multi-objective optimization formulation will be presented that creates best tender data to contractor comparing with more feasible options that are generated from multi-mode resources in a given project. This mathematical formulation is intended to give more scenarios which provide a practical support for typical construction contractors who need to optimize resource utilization in order to minimize project duration, project/bid price and project maximum working capital while maximizing its net present value simultaneously. At the end of the paper, an illustrative example will be presented to demonstrate the applications of proposed technique to an optimization expressway of repetitive construction project.

  2. Bonding of the silane containing multi-mode universal adhesive for lithium disilicate ceramics.

    Science.gov (United States)

    Lee, Hyun-Young; Han, Geum-Jun; Chang, Juhea; Son, Ho-Hyun

    2017-05-01

    This study evaluated the influence of a multi-mode universal adhesive (MUA) containing silane (Single Bond Universal, 3M EPSE) on the bonding of resin cement to lithium disilicate. Thirty IPS e.max CAD specimens (Ivoclar Vivadent) were fabricated. The surfaces were treated as follows: Group A, adhesive that did not contain silane (ANS, Porcelain Bonding Resin, Bisco); Group B, silane (S) and ANS; Group C, hydrofluoric acid (HF), S, and ANS; Group D, MUA; Group E, HF and MUA. Dual-cure resin cement (NX3, Kerr) was applied and composite resin cylinders of 0.8 mm in diameter were placed on it before light polymerization. Bonded specimens were stored in water for 24 hours or underwent a 10,000 thermocycling process prior to microshear bond strength testing. The data were analyzed using multivariate analysis of variance ( p silane increased the bond strength of resin cement. However, after thermocycling, the silane in MUA did not help achieve durable bond strength between lithium disilicate and resin cement, even when HF was applied.

  3. Experimental Investigation of Multi-mode Fiber Laser Cutting of Cement Mortar.

    Science.gov (United States)

    Lee, Dongkyoung; Pyo, Sukhoon

    2018-02-10

    This study successfully applied multi-mode laser cutting with the variation of the laser cutting speed to cement mortar for the first time. The effects of the amount of silica sand in the cement mortar on laser cutting are tested and analyzed. The kerf width and penetration depth of the specimens after laser cutting are investigated. As the laser cutting speed increases, the penetration depth decreases for both cement paste and cement mortar, whereas the kerf width becomes saturated and increases, respectively, for cement paste and cement mortar. Cross sections of the specimens are compared with illustrations. Top-view images of the cement mortar with indicators of the physical characteristics, such as re-solidification, burning, and cracks are examined, and the possible causes of these characteristics are explained. The optical absorption rates of cement-based materials are quantified at wide ranges of wavelength to compare the absorption rates in accordance with the materials compositions. The chemical composition variation before and after laser cutting is also compared by EDX (Energy Dispersive X-Ray) analysis. In addition to these observations, material removal mechanisms for cement mortar are proposed.

  4. Optimal Estimation of Diffusion Coefficients from Noisy Time-Lapse-Recorded Single-Particle Trajectories

    DEFF Research Database (Denmark)

    Vestergaard, Christian Lyngby

    2012-01-01

    Optimal Estimation of Diusion Coecients from Noisy Time-Lapse- Measurements of Single-Particle Trajectories Single-particle tracking techniques allow quantitative measurements of diusion at the single-molecule level. Recorded time-series are mostly short and contain considerable measurement noise....... The standard method for estimating diusion coecients from single-particle trajectories is based on leastsquares tting to the experimentally measured mean square displacements. This method is highly inecient, since it ignores the high correlations inherent in these. We derive the exact maximum likelihood...... parameter values. We extend the methods to particles diusing on a uctuating substrate, e.g., exible or semi exible polymers such as DNA, and show that uctuations induce an important bias in the estimates of diusion coecients if they are not accounted for. We apply the methods to obtain precise estimates...

  5. Centroids of effective interactions from measured single-particle energies: An application

    International Nuclear Information System (INIS)

    Cole, B.J.

    1990-01-01

    Centroids of the effective nucleon-nucleon interaction for the mass region A=28--64 are extracted directly from experimental single-particle spectra, by comparing single-particle energies relative to different cores. Uncertainties in the centroids are estimated at approximately 100 keV, except in cases of exceptional fragmentation of the single-particle strength. The use of a large number of inert cores allows the dependence of the interaction on mass or model space to be investigated. The method permits accurate empirical modifications to be made to realistic interactions calculated from bare nucleon-nucleon potentials, which are known to possess defective centroids in many cases. In addition, the centroids can be used as input to the more sophisticated fitting procedures that are employed to produce matrix elements of the effective interaction

  6. Understanding particle size and distance driven competition of interparticle interactions and effective single-particle anisotropy.

    Science.gov (United States)

    Pacakova, B; Mantlikova, A; Niznansky, D; Kubickova, S; Vejpravova, J

    2016-05-25

    Magnetic response of single-domain nanoparticles (NPs) in concentrated systems is strongly affected by mutual interparticle interactions. However, particle proximity significantly influences single-particle effective anisotropy. To solve which of these two phenomena plays a dominant role in the magnetic response of real NP systems, systematic study on samples with well-defined parameters is required. In our work, we prepared a series of nanocomposites constituted of highly-crystalline and well-isolated CoFe2O4 NPs embedded in an amorphous SiO2 matrix using a single-molecule precursor method. This preparation method enabled us to reach a wide interval of particle size and concentration. We observed that the characteristic parameters of the single-domain state (coercivity, blocking temperature) and dipole-dipole interaction energy ([Formula: see text]) scaled with each other and increased with increasing [Formula: see text], where d XRD was the NP diameter and r was the interparticle distance. Our results are in excellent agreement with Monte-Carlo simulations of the particle growth. Moreover, we demonstrated that the contribution of [Formula: see text] acting as an additional energetic barrier to the superspin reversal or as an average static field did not sufficiently explain how the concentrated NP systems responded to an external magnetic field. Alternations in the blocking temperature and coercivity of our NP systems accounted for reformed relaxations of the NP superspins and modified effective anisotropy energy of the interacting NPs. Therefore, the concept of modified NP effective anisotropy explains the magnetic response of our concentrated NP systems better than the concept of the energy barrier influenced by interparticle interactions.

  7. Single particle radiation between high spin states in /sup 147/Gd

    Energy Technology Data Exchange (ETDEWEB)

    Borggreen, J.; Sletten, G.; Bjoernholm, S.; Pedersen, J.; Del Zoppo, A.; Radford, D.C.; Janssens, R.V.F.; Chowdhury, P.; Emling, H.; Frekers, D.

    1987-05-04

    Transitions above the T/sub 1/2/=550 ns, 8.59 MeV isomer in /sup 147/Gd have been studied using the (/sup 30/Si, 5n) reaction. Results from ..gamma gamma.. coincidence, angular distribution and recoil distance measurements are combined to establish a level scheme up to 16.9 MeV and I approx. = 79/2. Single particle configurations are assigned on the basis of the deformed independent particle model. The single particle nature of the highest spin states and the apparent lack of collectivity is discussed.

  8. The application of single particle hydrodynamics in continuum models of multiphase flow

    Science.gov (United States)

    Decker, Rand

    1988-01-01

    A review of the application of single particle hydrodynamics in models for the exchange of interphase momentum in continuum models of multiphase flow is presented. Considered are the equations of motion for a laminar, mechanical two phase flow. Inherent to this theory is a model for the interphase exchange of momentum due to drag between the dispersed particulate and continuous fluid phases. In addition, applications of two phase flow theory to de-mixing flows require the modeling of interphase momentum exchange due to lift forces. The applications of single particle analysis in deriving models for drag and lift are examined.

  9. Preparation of Disease-Related Protein Assemblies for Single Particle Electron Microscopy.

    Science.gov (United States)

    Cameron Varano, A; Harafuji, Naoe; Dearnaley, William; Guay-Woodford, Lisa; Kelly, Deborah F

    2017-01-01

    Electron microscopy (EM) is a rapidly growing area of structural biology that permits us to decode biological assemblies at the nanoscale. To examine biological materials for single particle EM analysis, purified assemblies must be obtained using biochemical separation techniques. Here, we describe effective methodologies for isolating histidine (his)-tagged protein assemblies from the nucleus of disease-relevant cell lines. We further demonstrate how isolated assemblies are visualized using single particle EM techniques and provide representative results for each step in the process.

  10. Comparison of single-/few-/multi-mode 850 nm VCSELs for optical OFDM transmission.

    Science.gov (United States)

    Kao, Hsuan-Yun; Tsai, Cheng-Ting; Leong, Shan-Fong; Peng, Chun-Yen; Chi, Yu-Chieh; Huang, Jian Jang; Kuo, Hao-Chung; Shih, Tien-Tsorng; Jou, Jau-Ji; Cheng, Wood-Hi; Wu, Chao-Hsin; Lin, Gong-Ru

    2017-07-10

    For high-speed optical OFDM transmission applications, a comprehensive comparison of the homemade multi-/few-/single-transverse mode (MM/FM/SM) vertical cavity surface emitting laser (VCSEL) chips is performed. With microwave probe, the direct encoding of pre-leveled 16-QAM OFDM data and transmission over 100-m-long OM4 multi-mode-fiber (MMF) are demonstrated for intra-datacenter applications. The MM VCSEL chip with the largest emission aperture of 11 μm reveals the highest differential quantum efficiency which provides the highest optical power of 8.67 mW but exhibits the lowest encodable bandwidth of 21 GHz. In contrast, the SM VCSEL chip fabricated with the smallest emission aperture of only 3 μm provides the highest 3-dB encoding bandwidth up to 23 GHz at a cost of slight heat accumulation. After optimization, with the trade-off set between the receiving signal-to-noise ratio (SNR) and bandwidth, the FM VCSEL chip guarantees the highest optical OFDM transmission bit rate of 96 Gbit/s under back-to-back case with its strongest throughput. Among three VCSEL chips, the SM VCSEL chip with nearly modal-dispersion free feature is treated as the best candidate for carrying the pre-leveled 16-QAM OFDM data over 100-m OM4-MMF with same material structure but exhibits different oxide-layer confined gain cross-sections with one another at 80-Gbit/s with the smallest receiving power penalty of 1.77 dB.

  11. DECAY MODES OF HIGH-LYING SINGLE-PARTICLE STATES IN PB-209

    NARCIS (Netherlands)

    BEAUMEL, D; FORTIER, S; GALES, S; GUILLOT, J; LANGEVINJOLIOT, H; LAURENT, H; MAISON, JM; VERNOTTE, J; BORDEWIJK, JA; BRANDENBURG, S; KRASZNAHORKAY, A; CRAWLEY, GM; MASSOLO, CP; RENTERIA, M

    The neutron decay of high-lying single-particle states in Pb-209 excited by means of the (alpha, He-3) reaction has been investigated at 122 MeV incident energy using a multidetector array. The high-spin values of these states, inferred from previous inclusive experiments, are confirmed by the

  12. A new single-particle basis for nuclear many-body calculations

    Science.gov (United States)

    Puddu, G.

    2017-10-01

    Predominantly, harmonic oscillator single-particle wave functions are the preferred choice for a basis in ab initio nuclear many-body calculations. These wave-functions, although very convenient in order to evaluate the matrix elements of the interaction in the laboratory frame, have too fast a fall-off at large distances. In the past, as an alternative to the harmonic oscillator, other single-particle wave functions have been proposed. In this work, we propose a new single-particle basis, directly linked to nucleon-nucleon interaction. This new basis is orthonormal and complete, has the proper asymptotic behavior at large distances and does not contain the continuum which would pose severe convergence problems in nuclear many body calculations. We consider the newly proposed NNLO-opt nucleon-nucleon interaction, without any renormalization. We show that, unlike other bases, this single-particle representation has a computational cost similar to the harmonic oscillator basis with the same space truncation and it gives lower energies for 6He and 6Li.

  13. Summary report of the group on single-particle nonlinear dynamics

    International Nuclear Information System (INIS)

    Axinescu, S.; Bartolini, R.; Bazzani, A.

    1996-10-01

    This report summarizes the research on single-particle nonlinear beam dynamics. It discusses the following topics: analytical and semi-analytical tools; early prediction of the dynamic aperture; how the results are commonly presented; Is the mechanism of the dynamic aperture understand; ripple effects; and beam-beam effects

  14. Homogeneous vs heterogeneous polymerization catalysis revealed by single-particle fluorescence microscopy.

    Science.gov (United States)

    Esfandiari, N Melody; Blum, Suzanne A

    2011-11-16

    A high-sensitivity and high-resolution single-particle fluorescence microscopy technique differentiated between homogeneous and heterogeneous metathesis polymerization catalysis by imaging the location of the early stages of polymerization. By imaging single polymers and single crystals of Grubbs II, polymerization catalysis was revealed to be solely homogeneous rather than heterogeneous or both.

  15. Single-particle electron microscopy in the study of membrane protein structure.

    Science.gov (United States)

    De Zorzi, Rita; Mi, Wei; Liao, Maofu; Walz, Thomas

    2016-02-01

    Single-particle electron microscopy (EM) provides the great advantage that protein structure can be studied without the need to grow crystals. However, due to technical limitations, this approach played only a minor role in the study of membrane protein structure. This situation has recently changed dramatically with the introduction of direct electron detection device cameras, which allow images of unprecedented quality to be recorded, also making software algorithms, such as three-dimensional classification and structure refinement, much more powerful. The enhanced potential of single-particle EM was impressively demonstrated by delivering the first long-sought atomic model of a member of the biomedically important transient receptor potential channel family. Structures of several more membrane proteins followed in short order. This review recounts the history of single-particle EM in the study of membrane proteins, describes the technical advances that now allow this approach to generate atomic models of membrane proteins and provides a brief overview of some of the membrane protein structures that have been studied by single-particle EM to date. © The Author 2015. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  16. Single-particle thermal diffusion of charged colloids: Double-layer theory in a temperature gradient

    NARCIS (Netherlands)

    Dhont, J.K.G.; Briels, Willem J.

    2008-01-01

    The double-layer contribution to the single-particle thermal diffusion coefficient of charged, spherical colloids with arbitrary double-layer thickness is calculated and compared to experiments. The calculation is based on an extension of the Debye-Hückel theory for the double-layer structure that

  17. Lagrangian single-particle turbulent statistics through the Hilbert-Huang transform

    NARCIS (Netherlands)

    Huang, Y.; Biferale, L.; Calzavarini, E.; Sun, Chao; Toschi, F.

    2013-01-01

    The Hilbert-Huang transform is applied to analyze single-particle Lagrangian velocity data from numerical simulations of hydrodynamic turbulence. The velocity trajectory is described in terms of a set of intrinsic mode functions C i (t) and of their instantaneous frequency ω i (t) . On the basis of

  18. Insensitivity of single particle time domain measurements to laser velocimeter 'Doppler ambiguity.'

    Science.gov (United States)

    Johnson, D. A.

    1973-01-01

    It is shown that single particle time domain measurements in high speed gas flows obtained by a laser velocimeter technique developed for use in wind tunnels are not affected by the so-called 'Doppler ambiguity.' A comparison of hot-wire anemometer and laser velocimeter measurements taken under similar flow conditions is used for the demonstration.

  19. Single particle electron microscopy in combination with mass spectrometry to investigate novel complexes of membrane proteins

    NARCIS (Netherlands)

    Arteni, Ana A.; Nowaczyk, Marc; Lax, Julia; Rögner, Matthias; Boekema, Egbert J.; Kouril, R.; Rogner, M.

    2005-01-01

    Large data sets of molecular projections of the membrane proteins Photosystem I and Photosystem II from cyanobacteria were analyzed by single particle electron microscopy (EM). Analysis resulted in the averaging of 2D projections from the purified complexes but also in the simultaneous detection and

  20. Deformed single-particle levels in the boson-fermion model

    International Nuclear Information System (INIS)

    Leviatan, A.; Shao, B.

    1989-01-01

    Deformed single-particle levels are derived from a boson-fermion Hamiltonian in which the odd fermion occupies several j orbits. The geometric-oriented approach applied to 169 Tm clarified the role of algebraic interactions and provides an intuitive interpretation and guidance to numerical calculations in deformed nuclei

  1. Deformed single-particle levels in the boson-fermion model

    Energy Technology Data Exchange (ETDEWEB)

    Leviatan, A.; Shao, B. (Center for Theoretical Physics, Sloane Laboratory, Yale University, New Haven, Connecticut 06511 (US) Theoretical Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (US))

    1989-11-13

    Deformed single-particle levels are derived from a boson-fermion Hamiltonian in which the odd fermion occupies several {ital j} orbits. The geometric-oriented approach applied to {sup 169}Tm clarified the role of algebraic interactions and provides an intuitive interpretation and guidance to numerical calculations in deformed nuclei.

  2. Understanding particle size and distance driven competition of interparticle interactions and effective single-particle anisotropy

    Czech Academy of Sciences Publication Activity Database

    Pacáková, Barbara; Mantlíková, Alice; Nižňanský, D.; Kubíčková, Simona; Vejpravová, Jana

    2016-01-01

    Roč. 28, č. 20 (2016), 1-11, č. článku 206004. ISSN 0953-8984 R&D Projects: GA ČR(CZ) GA15-01953S Institutional support: RVO:68378271 Keywords : magnetic nanoparticles * single-particle anisotropy * dipolar energy Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 2.649, year: 2016

  3. Optical Splitters Based on Self-Imaging Effect in Multi-Mode Waveguide Made by Ion Exchange in Glass

    OpenAIRE

    Barkman, O.; Jerabek, V.; Prajzler, V.

    2013-01-01

    Design and modeling of single mode optical multi-mode interference structures with graded refractive index is reported. Several samples of planar optical channel waveguides were obtained by Ag+Na+ and K+Na+ one step thermal ion exchange process in molten salt on GIL49 glass substrate and new special optical glass for ion exchange technology. Waveguide properties were measured by optical mode spectroscopy. Obtained data were used for further design and modeling of single mode channel waveguide...

  4. An Entropy-Based Upper Bound Methodology for Robust Predictive Multi-Mode RCPSP Schedules

    Directory of Open Access Journals (Sweden)

    Angela Hsiang-Ling Chen

    2014-09-01

    Full Text Available Projects are an important part of our activities and regardless of their magnitude, scheduling is at the very core of every project. In an ideal world makespan minimization, which is the most commonly sought objective, would give us an advantage. However, every time we execute a project we have to deal with uncertainty; part of it coming from known sources and part remaining unknown until it affects us. For this reason, it is much more practical to focus on making our schedules robust, capable of handling uncertainty, and even to determine a range in which the project could be completed. In this paper we focus on an approach to determine such a range for the Multi-mode Resource Constrained Project Scheduling Problem (MRCPSP, a widely researched, NP-complete problem, but without adding any subjective considerations to its estimation. We do this by using a concept well known in the domain of thermodynamics, entropy and a three-stage approach. First we use Artificial Bee Colony (ABC—an effective and powerful meta-heuristic—to determine a schedule with minimized makespan which serves as a lower bound. The second stage defines buffer times and creates an upper bound makespan using an entropy function, with the advantage over other methods that it only considers elements which are inherent to the schedule itself and does not introduce any subjectivity to the buffer time generation. In the last stage, we use the ABC algorithm with an objective function that seeks to maximize robustness while staying within the makespan boundaries defined previously and in some cases even below the lower boundary. We evaluate our approach with two different benchmarks sets: when using the PSPLIB for the MRCPSP benchmark set, the computational results indicate that it is possible to generate robust schedules which generally result in an increase of less than 10% of the best known solutions while increasing the robustness in at least 20% for practically every

  5. Quantification of dermal exposure to nanoparticles from solid nanocomposites by using single particle ICP-MS

    DEFF Research Database (Denmark)

    Mackevica, Aiga; Olsson, Mikael Emil; Hansen, Steffen Foss

    2016-01-01

    was tested by surface wiping followed by analysis using single particle ICP-MS. The nanoparticles were extracted from the wipes by ultrasonication in deionized water, and this technique was tested to be around 60-100% effective for extracting the particles adsorbed to the wipes. The method was optimized......Engineered nanoparticles are used in various applications due to their unique properties, which has led to their widespread use in consumer products. Silver, titanium and copper-based nanoparticles are few of the most commonly used nanomaterials in consumer products, mainly due to their biocidal...... by spiking the wipes with known amounts of nanoparticles and treating them the same way as the experimental samples. Our preliminary results show that single particle ICP-MS has the potential for quantitatively measuring potential dermal exposure to nanoparticles, and when used in combination with other...

  6. Blowing Snow and Aerosol Composition: Bulk and Single Particle Measurements in Antarctica

    Science.gov (United States)

    DeCarlo, P. F.; Giordano, M.

    2017-12-01

    Recent evidence suggests that aerosol concentration and composition in the cryosphere is influenced by blowing snow, though the mechanisms remain unclear. Changes in aerosol composition due to blowing snow may significantly alter local and regional aerosol production, processing, transport, and lifetimes in the cryosphere. This presentation will focus on both bulk composition changes and single particle results from deploying an aerosol mass spectrometer (AMS) to the Antarctic sea ice during the 2ODIAC campaign, with a focus on blowing snow events. With this first on-line analysis, blowing snow clearly enhances the submicron sea salt (Na and Cl) concentrations in Antarctic aerosols. These bulk composition changes are shown to be independent from air mass origins. Single particle results from the AMS show a variety of chemical species in addition to sulfates in the submicron aerosol mass. K-means cluster analysis also shows distinct changes in the overall aerosol mass spectra during to blowing snow events.

  7. Single-particle model of a strongly driven, dense, nanoscale quantum ensemble

    Science.gov (United States)

    DiLoreto, C. S.; Rangan, C.

    2018-01-01

    We study the effects of interatomic interactions on the quantum dynamics of a dense, nanoscale, atomic ensemble driven by a strong electromagnetic field. We use a self-consistent, mean-field technique based on the pseudospectral time-domain method and a full, three-directional basis to solve the coupled Maxwell-Liouville equations. We find that interatomic interactions generate a decoherence in the state of an ensemble on a much faster time scale than the excited-state lifetime of individual atoms. We present a single-particle model of the driven, dense ensemble by incorporating interactions into a dephasing rate. This single-particle model reproduces the essential physics of the full simulation and is an efficient way of rapidly estimating the collective dynamics of a dense ensemble.

  8. The advent of structural biologyin situby single particle cryo-electron tomography.

    Science.gov (United States)

    Galaz-Montoya, Jesús G; Ludtke, Steven J

    2017-01-01

    Single particle tomography (SPT), also known as subtomogram averaging, is a powerful technique uniquely poised to address questions in structural biology that are not amenable to more traditional approaches like X-ray crystallography, nuclear magnetic resonance, and conventional cryoEM single particle analysis. Owing to its potential for in situ structural biology at subnanometer resolution, SPT has been gaining enormous momentum in the last five years and is becoming a prominent, widely used technique. This method can be applied to unambiguously determine the structures of macromolecular complexes that exhibit compositional and conformational heterogeneity, both in vitro and in situ . Here we review the development of SPT, highlighting its applications and identifying areas of ongoing development.

  9. Comparison of mineral dust and droplet residuals measured with two single particle aerosol mass spectrometers

    Science.gov (United States)

    Wonaschütz, Anna; Ludwig, Wolfgang; Zawadowicz, Maria; Hiranuma, Naruki; Hitzenberger, Regina; Cziczo, Daniel; DeMott, Paul; Möhler, Ottmar

    2017-04-01

    Single Particle mass spectrometers are used to gain information on the chemical composition of individual aerosol particles, aerosol mixing state, and other valuable aerosol characteristics. During the Mass Spectrometry Intercomparison at the Fifth Ice Nucleation (FIN-01) Workshop, the new LAAPTOF single particle aerosol mass spectrometer (AeroMegt GmbH) was conducting simultaneous measurements together with the PALMS (Particle Analysis by Laser Mass Spectrometry) instrument. The aerosol particles were sampled from the AIDA chamber during ice cloud expansion experiments. Samples of mineral dust and ice droplet residuals were measured simultaneously. In this work, three expansion experiments are chosen for a comparison between the two mass spectrometers. A fuzzy clustering routine is used to group the spectra. Cluster centers describing the ensemble of particles are compared. First results show that while differences in the peak heights are likely due to the use of an amplifier in PALMS, cluster centers are comparable.

  10. Insight into interrelation between single-particle and collective diffusion in binary melts

    Science.gov (United States)

    Levchenko, Elena V.; Evteev, Alexander V.

    2018-01-01

    The interrelation between the kinetics of single-particle (tracer) and collective diffusion in a binary melt is investigated theoretically within the framework of the Mori-Zwanzig formalism of statistical mechanics. An analytical expression for the Onsager coefficient for mass transport and two self-diffusion coefficients of species in a binary melt is derived using analysis based on the generalized Langevin equation. The derived expression naturally accounts for manifestation of microscopic (dynamic) cross-correlation effects in the kinetics of collective diffusion. Hence, it presents an explicit extension of the well-known Darken equation which is currently often used for expressing collective interdiffusion in terms of the two self-diffusion coefficients. An application of our analysis for interpretation of recent experimental data on the interrelation between the kinetics of single-particle and collective diffusion in Al-rich Ni-Al melts is demonstrated.

  11. Innovative molecular-based fluorescent nanoparticles for multicolor single particle tracking in cells

    International Nuclear Information System (INIS)

    Daniel, Jonathan; Blanchard-Desce, Mireille; Godin, Antoine G; Palayret, Matthieu; Lounis, Brahim; Cognet, Laurent

    2016-01-01

    Based on an original molecular-based design, we present bright and photostable fluorescent organic nanoparticles (FONs) showing excellent colloidal stability in various aqueous environments. Complementary near-infrared emitting and green emitting FONs were prepared using a simple, fast and robust protocol. Both types of FONs could be simultaneously imaged at the single-particle level in solution as well as in biological environments using a monochromatic excitation and a dual-color fluorescence microscope. No evidence of acute cytotoxicity was found upon incubation of live cells with mixed solutions of FONs, and both types of nanoparticles were found internalized in the cells where their motion could be simultaneously tracked at video-rate up to minutes. These fluorescent organic nanoparticles open a novel non-toxic alternative to existing nanoparticles for imaging biological structures, compatible with live-cell experiments and specially fitted for multicolor single particle tracking. (paper)

  12. Radiative capture of nucleons at astrophysical energies with single-particle states

    International Nuclear Information System (INIS)

    Huang, J.T.; Bertulani, C.A.; Guimaraes, V.

    2010-01-01

    Radiative capture of nucleons at energies of astrophysical interest is one of the most important processes for nucleosynthesis. The nucleon capture can occur either by a compound nucleus reaction or by a direct process. The compound reaction cross sections are usually very small, especially for light nuclei. The direct capture proceeds either via the formation of a single-particle resonance or a non-resonant capture process. In this work we calculate radiative capture cross sections and astrophysical S-factors for nuclei in the mass region A<20 using single-particle states. We carefully discuss the parameter fitting procedure adopted in the simplified two-body treatment of the capture process. Then we produce a detailed list of cases for which the model works well. Useful quantities, such as spectroscopic factors and asymptotic normalization coefficients, are obtained and compared to published data.

  13. A clustering approach to multireference alignment of single-particle projections in electron microscopy

    OpenAIRE

    Sorzano, C.O.S.; Bilbao-Castro, J.R.; Shkolnisky, Y.; Alcorlo, M.; Melero, R.; Caffarena-Fernández, G.; Li, M.; Xu, G.; Marabini, R.; Carazo, J.M.

    2010-01-01

    Two-dimensional analysis of projections of single particles acquired by an electron microscope is a useful tool to help identifying the different kinds of projections present in a dataset and their different projection directions. Such analysis is also useful to distinguish between different kinds of particles or different particle conformations. In this paper we introduce a new algorithm for performing two-dimensional multireference alignment and classification that is based on a Hierarchica...

  14. Single particle measurements and two particle interferometry results from CERN experiment NA44

    International Nuclear Information System (INIS)

    Simon-Gillo, J.

    1994-01-01

    CERN experiment NA44 is optimized for the study of identified single and multiple particle distributions to p T = 0 near mid-rapidity. We measure π +- , K +- , p, bar p, d and bar d, in p + A and A + A collisions at 450 and 20OGeV/u, respectively. Two-particle intensity interferometry results from π + π + , K + K + , and K - K - measurements and single particle distributions are presented

  15. A deep convolutional neural network approach to single-particle recognition in cryo-electron microscopy.

    Science.gov (United States)

    Zhu, Yanan; Ouyang, Qi; Mao, Youdong

    2017-07-21

    Single-particle cryo-electron microscopy (cryo-EM) has become a mainstream tool for the structural determination of biological macromolecular complexes. However, high-resolution cryo-EM reconstruction often requires hundreds of thousands of single-particle images. Particle extraction from experimental micrographs thus can be laborious and presents a major practical bottleneck in cryo-EM structural determination. Existing computational methods for particle picking often use low-resolution templates for particle matching, making them susceptible to reference-dependent bias. It is critical to develop a highly efficient template-free method for the automatic recognition of particle images from cryo-EM micrographs. We developed a deep learning-based algorithmic framework, DeepEM, for single-particle recognition from noisy cryo-EM micrographs, enabling automated particle picking, selection and verification in an integrated fashion. The kernel of DeepEM is built upon a convolutional neural network (CNN) composed of eight layers, which can be recursively trained to be highly "knowledgeable". Our approach exhibits an improved performance and accuracy when tested on the standard KLH dataset. Application of DeepEM to several challenging experimental cryo-EM datasets demonstrated its ability to avoid the selection of un-wanted particles and non-particles even when true particles contain fewer features. The DeepEM methodology, derived from a deep CNN, allows automated particle extraction from raw cryo-EM micrographs in the absence of a template. It demonstrates an improved performance, objectivity and accuracy. Application of this novel method is expected to free the labor involved in single-particle verification, significantly improving the efficiency of cryo-EM data processing.

  16. Quantum private comparison with d-level single-particle states

    International Nuclear Information System (INIS)

    Yu, Chao-Hua; Guo, Gong-De; Lin, Song

    2013-01-01

    In this paper, a quantum private comparison protocol with d-level single-particle states is proposed. In the protocol, a semi-honest third party is introduced to help two participants compare the size relationship of their secrets without revealing them to any other people. It is shown that the protocol is secure in theory. Moreover, the security of the protocol in real circumstance is also discussed. (paper)

  17. Single-particle cryo-electron microscopy of Rift Valley fever virus

    OpenAIRE

    Sherman, Michael B.; Freiberg, Alexander N.; Holbrook, Michael R.; Watowich, Stanley J.

    2009-01-01

    Rift Valley fever virus (RVFV; Bunyaviridae; Phlebovirus) is an emerging human veterinary pathogen causing acute hepatitis in ruminants and has the potential to Single-particle cryo-EM reconstruction of RVFV MP-12 hemorrhagic fever in humans. We report a three-dimensional reconstruction of RVFV vaccine strain MP-12 (RVFV MP-12) by cryo-electron microcopy using icosahedral symmetry of individual virions. Although the genomic core of RVFV MP-12 is apparently poorly ordered, the glycoproteins on...

  18. A new seniority scheme for non-degenerate single particle orbits

    International Nuclear Information System (INIS)

    Otsuka, T.; Arima, A.

    1978-01-01

    A new method is proposed in the treatment of the seniority scheme. The method enables one to evaluate analytically the contribution from J = 0 Cooper pairs in non-degenerate single-particle orbits to many-body matrix elements. It includes the SU(2) quasi-spin and the BCS approximation as two extreme limits. The effect of particle number conservation is properly taken into account. (Auth.)

  19. High rate discharge capability of single particle electrode of LiCoO{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Dokko, Kaoru [Department of Chemistry and Biotechnology, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama 240-8501 (Japan); Nakata, Natsuko; Kanamura, Kiyoshi [Department of Applied Chemistry, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachioji, Tokyo 192-0397 (Japan)

    2009-04-01

    The electrochemical properties of a single particle of LiCoO{sub 2} (8 {mu}m in diameter) in an organic electrolyte were characterized using a microelectrode technique, and the high rate capability of commercially available micron-sized LiCoO{sub 2} was examined in this study. A Pt microfilament (10 {mu}m in diameter) was attached to the single LiCoO{sub 2} particle in the electrolyte during optical microscope observation, and galvanostatic charge-discharge tests were carried out. The discharge capacity of the single LiCoO{sub 2} particle (8 {mu}m diameter) was 0.157 nA h in the potential range of 3.0-4.2 V vs. Li/Li{sup +}, which was close to the theoretical capacity. The discharge rate capability of the single LiCoO{sub 2} particle was excellent, and the particle exhibited its full-discharge capacity up to a high rate of 30 C (5 nA). The discharge reaction of the single particle was not controlled by the solid-state diffusion of Li{sup +}, but by the charge transfer process at a rate lower than 30 C. The discharge capacity of the particle measured at a high rate of 300 C (50 nA) was 0.12 nA h, which was more than 75% of the full capacity of a single particle. (author)

  20. High rate discharge capability of single particle electrode of LiCoO 2

    Science.gov (United States)

    Dokko, Kaoru; Nakata, Natsuko; Kanamura, Kiyoshi

    The electrochemical properties of a single particle of LiCoO 2 (8 μm in diameter) in an organic electrolyte were characterized using a microelectrode technique, and the high rate capability of commercially available micron-sized LiCoO 2 was examined in this study. A Pt microfilament (10 μm in diameter) was attached to the single LiCoO 2 particle in the electrolyte during optical microscope observation, and galvanostatic charge-discharge tests were carried out. The discharge capacity of the single LiCoO 2 particle (8 μm diameter) was 0.157 nA h in the potential range of 3.0-4.2 V vs. Li/Li +, which was close to the theoretical capacity. The discharge rate capability of the single LiCoO 2 particle was excellent, and the particle exhibited its full-discharge capacity up to a high rate of 30 C (5 nA). The discharge reaction of the single particle was not controlled by the solid-state diffusion of Li +, but by the charge transfer process at a rate lower than 30 C. The discharge capacity of the particle measured at a high rate of 300 C (50 nA) was 0.12 nA h, which was more than 75% of the full capacity of a single particle.

  1. A new Insight Into Microscale Soil Organic Matter Dynamics - From Single Particles to Aggregates

    Science.gov (United States)

    Mueller, C. W.; Heister, K.; Hillion, F.; Herrmann, A. M.; Koegel-Knabner, I.

    2008-12-01

    Both mineral interactions and the spatial inaccessibility due to aggregation are key-factors affecting the stabilization of soil organic matter (SOM). Knowledge about the factors controlling the preservation of SOM and underlying stabilization mechanisms has improved significantly over the last years. Nevertheless, in situ processes remain almost unclear and are still challenging to evaluate. In the presented work, we studied the alteration of spatial distribution of fresh introduced OM over time on single particles and in intact soil aggregates. Single particles of a fine silt and clay mixture (resin embedded. Samples were then analyzed by scanning electron microscopy (SEM) and nano-scale secondary ion mass spectrometry (nanoSIMS50). We will demonstrate the spatial distribution of OM on single particles and in intact soil aggregates at the microscale by SEM and nanoSIMS. In addition, with the isotopic sensitivity of nanoSIMS, we are able to follow the fate of 13C and 15N, which is expected to be influenced by diffusion, sorption and microbial activity. From these results, we propose how OM in soil can be stabilized on single soil particles and at complex soil aggregates.

  2. Single-particle cryo-EM-Improved ab initio 3D reconstruction with SIMPLE/PRIME.

    Science.gov (United States)

    Reboul, Cyril F; Eager, Michael; Elmlund, Dominika; Elmlund, Hans

    2018-01-01

    Cryogenic electron microscopy (cryo-EM) and single-particle analysis now enables the determination of high-resolution structures of macromolecular assemblies that have resisted X-ray crystallography and other approaches. We developed the SIMPLE open-source image-processing suite for analysing cryo-EM images of single-particles. A core component of SIMPLE is the probabilistic PRIME algorithm for identifying clusters of images in 2D and determine relative orientations of single-particle projections in 3D. Here, we extend our previous work on PRIME and introduce new stochastic optimization algorithms that improve the robustness of the approach. Our refined method for identification of homogeneous subsets of images in accurate register substantially improves the resolution of the cluster centers and of the ab initio 3D reconstructions derived from them. We now obtain maps with a resolution better than 10 Å by exclusively processing cluster centers. Excellent parallel code performance on over-the-counter laptops and CPU workstations is demonstrated. © 2017 The Protein Society.

  3. Online single particle measurement of fireworks pollution during Chinese New Year in Nanning.

    Science.gov (United States)

    Li, Jingyan; Xu, Tingting; Lu, Xiaohui; Chen, Hong; Nizkorodov, Sergey A; Chen, Jianmin; Yang, Xin; Mo, Zhaoyu; Chen, Zhiming; Liu, Huilin; Mao, Jingying; Liang, Guiyun

    2017-03-01

    Time-resolved single-particle measurements were conducted during Chinese New Year in Nanning, China. Firework displays resulted in a burst of SO 2 , coarse mode, and accumulation mode (100-500nm) particles. Through single particle mass spectrometry analysis, five different types of particles (fireworks-metal, ash, dust, organic carbon-sulfate (OC-sulfate), biomass burning) with different size distributions were identified as primary emissions from firework displays. The fireworks-related particles accounted for more than 70% of the total analyzed particles during severe firework detonations. The formation of secondary particulate sulfate and nitrate during firework events was investigated on single particle level. An increase of sulfite peak (80SO 3 - ) followed by an increase of sulfate peaks (97HSO 4 - +96SO 4 - ) in the mass spectra during firework displays indicated the aqueous uptake and oxidation of SO 2 on particles. High concentration of gaseous SO 2 , high relative humidity and high particle loading likely promoted SO 2 oxidation. Secondary nitrate formed through gas-phase oxidation of NO 2 to nitric acid, followed by the condensation into particles as ammonium nitrate. This study shows that under worm, humid conditions, both primary and secondary aerosols contribute to the particulate air pollution during firework displays. Copyright © 2016. Published by Elsevier B.V.

  4. Exploring Electronic Structure and Order in Polymers via Single-Particle Microresonator Spectroscopy.

    Science.gov (United States)

    Horak, Erik H; Rea, Morgan T; Heylman, Kevin D; Gelbwaser-Klimovsky, David; Saikin, Semion K; Thompson, Blaise J; Kohler, Daniel D; Knapper, Kassandra A; Wei, Wei; Pan, Feng; Gopalan, Padma; Wright, John C; Aspuru-Guzik, Alán; Goldsmith, Randall H

    2018-02-08

    PSS, a transparent electrically conductive polymer, finds widespread use in electronic devices. While empirical efforts have increased conductivity, a detailed understanding of the coupled electronic and morphological landscapes in PEDOT:PSS has lagged due to substantial structural heterogeneity on multiple length-scales. We use an optical microresonator-based absorption spectrometer to perform single-particle measurements, providing a bottom-up examination of electronic structure and morphology ranging from single PEDOT:PSS polymers to nascent films. Using single-particle spectroscopy with complementary theoretical calculations and ultrafast spectroscopy, we demonstrate that PEDOT:PSS displays bulk-like optical response even in single polymers. We find highly ordered PEDOT assemblies with long-range ordering mediated by the insulating PSS matrix and reveal a preferential surface orientation of PEDOT nanocrystallites absent in bulk films with implications for interfacial electronic communication. Our single-particle perspective provides a unique window into the microscopic structure and electronic properties of PEDOT:PSS.

  5. Automatic CTF correction for single particles based upon multivariate statistical analysis of individual power spectra.

    Science.gov (United States)

    Sander, B; Golas, M M; Stark, H

    2003-06-01

    Three-dimensional electron cryomicroscopy of randomly oriented single particles is a method that is suitable for the determination of three-dimensional structures of macromolecular complexes at molecular resolution. However, the electron-microscopical projection images are modulated by a contrast transfer function (CTF) that prevents the calculation of three-dimensional reconstructions of biological complexes at high resolution from uncorrected images. We describe here an automated method for the accurate determination and correction of the CTF parameters defocus, twofold astigmatism and amplitude-contrast proportion from single-particle images. At the same time, the method allows the frequency-dependent signal decrease (B factor) and the non-convoluted background signal to be estimated. The method involves the classification of the power spectra of single-particle images into groups with similar CTF parameters; this is done by multivariate statistical analysis (MSA) and hierarchically ascending classification (HAC). Averaging over several power spectra generates class averages with enhanced signal-to-noise ratios. The correct CTF parameters can be deduced from these class averages by applying an iterative correlation procedure with theoretical CTF functions; they are then used to correct the raw images. Furthermore, the method enables the tilt axis of the sample holder to be determined and allows the elimination of individual poor-quality images that show high drift or charging effects.

  6. Single Particle ICP-MS: Advances toward routine analysis of nanomaterials.

    Science.gov (United States)

    Montaño, Manuel D; Olesik, John W; Barber, Angela G; Challis, Katie; Ranville, James F

    2016-07-01

    From its early beginnings in characterizing aerosol particles to its recent applications for investigating natural waters and waste streams, single particle inductively coupled plasma-mass spectrometry (spICP-MS) has proven to be a powerful technique for the detection and characterization of aqueous dispersions of metal-containing nanomaterials. Combining the high-throughput of an ensemble technique with the specificity of a single particle counting technique and the elemental specificity of ICP-MS, spICP-MS is capable of rapidly providing researchers with information pertaining to size, size distribution, particle number concentration, and major elemental composition with minimal sample perturbation. Recently, advances in data acquisition, signal processing, and the implementation of alternative mass analyzers (e.g., time-of-flight) has resulted in a wider breadth of particle analyses and made significant progress toward overcoming many of the challenges in the quantitative analysis of nanoparticles. This review provides an overview of spICP-MS development from a niche technique to application for routine analysis, a discussion of the key issues for quantitative analysis, and examples of its further advancement for analysis of increasingly complex environmental and biological samples. Graphical Abstract Single particle ICP-MS workflow for the analysis of suspended nanoparticles.

  7. Determining Complex Structures using Docking Method with Single Particle Scattering Data

    Directory of Open Access Journals (Sweden)

    Haiguang Liu

    2017-04-01

    Full Text Available Protein complexes are critical for many molecular functions. Due to intrinsic flexibility and dynamics of complexes, their structures are more difficult to determine using conventional experimental methods, in contrast to individual subunits. One of the major challenges is the crystallization of protein complexes. Using X-ray free electron lasers (XFELs, it is possible to collect scattering signals from non-crystalline protein complexes, but data interpretation is more difficult because of unknown orientations. Here, we propose a hybrid approach to determine protein complex structures by combining XFEL single particle scattering data with computational docking methods. Using simulations data, we demonstrate that a small set of single particle scattering data collected at random orientations can be used to distinguish the native complex structure from the decoys generated using docking algorithms. The results also indicate that a small set of single particle scattering data is superior to spherically averaged intensity profile in distinguishing complex structures. Given the fact that XFEL experimental data are difficult to acquire and at low abundance, this hybrid approach should find wide applications in data interpretations.

  8. Determining Complex Structures using Docking Method with Single Particle Scattering Data.

    Science.gov (United States)

    Wang, Hongxiao; Liu, Haiguang

    2017-01-01

    Protein complexes are critical for many molecular functions. Due to intrinsic flexibility and dynamics of complexes, their structures are more difficult to determine using conventional experimental methods, in contrast to individual subunits. One of the major challenges is the crystallization of protein complexes. Using X-ray free electron lasers (XFELs), it is possible to collect scattering signals from non-crystalline protein complexes, but data interpretation is more difficult because of unknown orientations. Here, we propose a hybrid approach to determine protein complex structures by combining XFEL single particle scattering data with computational docking methods. Using simulations data, we demonstrate that a small set of single particle scattering data collected at random orientations can be used to distinguish the native complex structure from the decoys generated using docking algorithms. The results also indicate that a small set of single particle scattering data is superior to spherically averaged intensity profile in distinguishing complex structures. Given the fact that XFEL experimental data are difficult to acquire and at low abundance, this hybrid approach should find wide applications in data interpretations.

  9. Fluidity evaluation of cell membrane model formed on graphene oxide with single particle tracking using quantum dot

    Science.gov (United States)

    Okamoto, Yoshiaki; Motegi, Toshinori; Iwasa, Seiji; Sandhu, Adarsh; Tero, Ryugo

    2015-04-01

    The lipid bilayer is the fundamental structure of plasma membranes, and artificial lipid bilayer membranes are used as model systems of cell membranes. Recently we reported the formation of a supported lipid bilayer (SLB) on graphene oxide (GO) by the vesicle fusion method. In this study, we conjugated a quantum dot (Qdot) on the SLB surface as a fluorescence probe brighter than dye-labeled lipid molecules, to qualitatively evaluate the fluidity of the SLB on GO by the single particle tracking method. We obtained the diffusion coefficient of the Qdot-conjugated lipids in the SLB on GO. We also performed the Qdot conjugation on the SLB containing a lipid conjugated with polyethylene glycol, to prevent the nonspecific adsorption of Qdots. The difference in the diffusion coefficients between the SLBs on the GO and the bare SiO2 regions was evaluated from the trajectory of single Qdot-conjugated lipid diffusing between the two regions.

  10. Optical Splitters Based on Self-Imaging Effect in Multi-Mode Waveguide Made by Ion Exchange in Glass

    Directory of Open Access Journals (Sweden)

    O. Barkman

    2013-04-01

    Full Text Available Design and modeling of single mode optical multi-mode interference structures with graded refractive index is reported. Several samples of planar optical channel waveguides were obtained by Ag+, Na+ and K+, Na+ one step thermal ion exchange process in molten salt on GIL49 glass substrate and new special optical glass for ion exchange technology. Waveguide properties were measured by optical mode spectroscopy. Obtained data were used for further design and modeling of single mode channel waveguide and subsequently for the design of 1 to 3 multimode interference power splitter in order to improve simulation accuracy. Designs were developed by utilizing finite difference beam propagation method.

  11. Single particle electron microscopy reconstruction of the exosome complex using the random conical tilt method.

    Science.gov (United States)

    Liu, Xueqi; Wang, Hong-Wei

    2011-03-28

    Single particle electron microscopy (EM) reconstruction has recently become a popular tool to get the three-dimensional (3D) structure of large macromolecular complexes. Compared to X-ray crystallography, it has some unique advantages. First, single particle EM reconstruction does not need to crystallize the protein sample, which is the bottleneck in X-ray crystallography, especially for large macromolecular complexes. Secondly, it does not need large amounts of protein samples. Compared with milligrams of proteins necessary for crystallization, single particle EM reconstruction only needs several micro-liters of protein solution at nano-molar concentrations, using the negative staining EM method. However, despite a few macromolecular assemblies with high symmetry, single particle EM is limited at relatively low resolution (lower than 1 nm resolution) for many specimens especially those without symmetry. This technique is also limited by the size of the molecules under study, i.e. 100 kDa for negatively stained specimens and 300 kDa for frozen-hydrated specimens in general. For a new sample of unknown structure, we generally use a heavy metal solution to embed the molecules by negative staining. The specimen is then examined in a transmission electron microscope to take two-dimensional (2D) micrographs of the molecules. Ideally, the protein molecules have a homogeneous 3D structure but exhibit different orientations in the micrographs. These micrographs are digitized and processed in computers as "single particles". Using two-dimensional alignment and classification techniques, homogenous molecules in the same views are clustered into classes. Their averages enhance the signal of the molecule's 2D shapes. After we assign the particles with the proper relative orientation (Euler angles), we will be able to reconstruct the 2D particle images into a 3D virtual volume. In single particle 3D reconstruction, an essential step is to correctly assign the proper orientation

  12. Detection of zinc oxide and cerium dioxide nanoparticles during drinking water treatment by rapid single particle ICP-MS methods.

    Science.gov (United States)

    Donovan, Ariel R; Adams, Craig D; Ma, Yinfa; Stephan, Chady; Eichholz, Todd; Shi, Honglan

    2016-07-01

    Nanoparticles (NPs) entering water systems are an emerging concern as NPs are more frequently manufactured and used. Single particle inductively coupled plasma-mass spectrometry (SP-ICP-MS) methods were validated to detect Zn- and Ce-containing NPs in surface and drinking water using a short dwell time of 0.1 ms or lower, ensuring precision in single particle detection while eliminating the need for sample preparation. Using this technique, information regarding NP size, size distribution, particle concentration, and dissolved ion concentrations was obtained simultaneously. The fates of Zn- and Ce-NPs, including those found in river water and added engineered NPs, were evaluated by simulating a typical drinking water treatment process. Lime softening, alum coagulation, powdered activated carbon sorption, and disinfection by free chlorine were simulated sequentially using river water. Lime softening removed 38-53 % of Zn-containing and ZnO NPs and >99 % of Ce-containing and CeO2 NPs. Zn-containing and ZnO NP removal increased to 61-74 % and 77-79 % after alum coagulation and disinfection, respectively. Source and drinking water samples were collected from three large drinking water treatment facilities and analyzed for Zn- and Ce-containing NPs. Each facility had these types of NPs present. In all cases, particle concentrations were reduced by a minimum of 60 % and most were reduced by >95 % from source water to finished drinking water. This study concludes that uncoated ZnO and CeO2 NPs may be effectively removed by conventional drinking water treatments including lime softening and alum coagulation.

  13. Graphene oxide and shape-controlled silver nanoparticle hybrids for ultrasensitive single-particle surface-enhanced Raman scattering (SERS) sensing.

    Science.gov (United States)

    Fan, Wei; Lee, Yih Hong; Pedireddy, Srikanth; Zhang, Qi; Liu, Tianxi; Ling, Xing Yi

    2014-05-07

    Graphene oxide (GO) is an emerging material for surface-enhanced Raman scattering (SERS) due to its strong chemical enhancement. Studying the SERS performance of plasmonic nanoparticle/GO hybrid materials at the single particle level is crucial for direct probing of the chemical effect of GO on plasmonic nanoparticles. In this work, we integrate GO and shape-controlled Ag nanoparticles to create hybrid nanomaterials, and the chemical enhancement arising from GO is investigated using single-particle SERS measurements. Ag nanoparticle@GO hybrid nanostructures are prepared by assembling Ag nanoparticles, including spheres, cubes and octahedra with GO sheets. The SERS behaviors of the hybrid nanostructures are characterized, and 2-3 times enhanced SERS intensities are detected from the Ag nanoparticle@GO hybrid nanostructures as compared to pure Ag nanoparticles. Furthermore, we probe the mechanism of SERS enhancement in the hybrid nanostructures by changing the surface coverage of GO on Ag octahedra, by using reduced GO in place of GO as well as by using probe molecules of different electronegativities. This hybrid system is an excellent candidate for single-particle SERS sensors. Sub-nanomolar levels of aromatic molecules are detected using a single Ag/GO hybrid nanomaterial. This as-prepared GO and shape-controlled Ag nanoparticle hybrid is capable of serving as a high performance SERS platform, providing new opportunities for efficient chemical and biological sensing applications.

  14. Building the Nanoplasmonics Toolbox Through Shape Modeling and Single Particle Optical Studies

    Science.gov (United States)

    Ringe, Emilie

    Interest in nanotechnology is driven by unprecedented properties tailorability, achievable by controlling particle structure and composition. Unlike bulk components, minute changes in size and shape affect the optical and electronic properties of nanoparticles. Characterization of such structure-function relationships and better understanding of structure control mechanisms is crucial to the development of applications such as plasmonic sensors and devices. The objective of the current research is thus twofold: to theoretically predict and understand how shape is controlled by synthesis conditions, and to experimentally unravel, through single particle studies, how shape, composition, size, and surrounding environment affect plasmonic properties in noble metal particles. Quantitative, predictive rules and fundamental knowledge obtained from this research contributes to the "nanoplasmonics toolbox", a library designed to provide scientists and engineers the tools to create and optimize novel nanotechnology applications. In this dissertation, single particle approaches are developed and used to unravel the effects of size, shape, substrate, aggregation state and surrounding environment on the optical response of metallic nanoparticles. Ag and Au nanocubes on different substrates are first presented, followed by the discussion of the concept of plasmon length, a universal parameter to describe plasmon energy for a variety of particle shapes and plasmon modes. Plasmonic sensing (both refractive index sensing and surface-enhanced Raman spectroscopy) and polarization effects are then studied at the single particle level. In the last two Chapters, analytical shape models based on the Wulff construction provide unique modeling tools for alloy and kinetically grown nanoparticles. The former reveals a size-dependence of the shape of small alloy particles (such as those used in catalysis) because of surface segregation, while the latter uniquely models the shape of many

  15. Single particle 3D reconstruction for 2D crystal images of membrane proteins.

    Science.gov (United States)

    Scherer, Sebastian; Arheit, Marcel; Kowal, Julia; Zeng, Xiangyan; Stahlberg, Henning

    2014-03-01

    In cases where ultra-flat cryo-preparations of well-ordered two-dimensional (2D) crystals are available, electron crystallography is a powerful method for the determination of the high-resolution structures of membrane and soluble proteins. However, crystal unbending and Fourier-filtering methods in electron crystallography three-dimensional (3D) image processing are generally limited in their performance for 2D crystals that are badly ordered or non-flat. Here we present a single particle image processing approach, which is implemented as an extension of the 2D crystallographic pipeline realized in the 2dx software package, for the determination of high-resolution 3D structures of membrane proteins. The algorithm presented, addresses the low single-to-noise ratio (SNR) of 2D crystal images by exploiting neighborhood correlation between adjacent proteins in the 2D crystal. Compared with conventional single particle processing for randomly oriented particles, the computational costs are greatly reduced due to the crystal-induced limited search space, which allows a much finer search space compared to classical single particle processing. To reduce the considerable computational costs, our software features a hybrid parallelization scheme for multi-CPU clusters and computer with high-end graphic processing units (GPUs). We successfully apply the new refinement method to the structure of the potassium channel MloK1. The calculated 3D reconstruction shows more structural details and contains less noise than the map obtained by conventional Fourier-filtering based processing of the same 2D crystal images. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

  16. Impact of KCl impregnation on single particle combustion of wood and torrefied wood

    DEFF Research Database (Denmark)

    Lu, Zhimin; Jian, Jie; Jensen, Peter Arendt

    2017-01-01

    In this work, single particle combustion of raw and torrefied 4 mm wood particles with different potassium content obtained by KCl impregnation and washing was studied experimentally under a condition of 1225 °C, 3.1% O2 and 26.1% H2O. The ignition time and devolatilization time depended almost......, and unchanged by torrefaction. Compared to the raw wood particle, the char conversion time was increased by torrefaction, decreased by washing, and almost unchanged by KCl impregnation due to its promoting effect on both char yield and reactivity....

  17. Statistical and direct decay of high-lying single-particle excitations

    International Nuclear Information System (INIS)

    Gales, S.

    1993-01-01

    Transfer reactions induced by hadronic probes at intermediate energies have revealed a rich spectrum of high-lying excitations embedded in the nuclear continuum. The investigation of their decay properties is believed to be a severe test of their microscopic structure as predicted by microscopic nuclear models. In addition the degree of damping of these simple modes in the nuclear continuum can be obtained by means of the measured particle (n,p) decay branching ratios. The neutron and proton decay studies of high-lying single-particle states in heavy nuclei are presented. (author). 13 refs., 9 figs

  18. The free-electron laser - Maxwell's equations driven by single-particle currents

    Science.gov (United States)

    Colson, W. B.; Ride, S. K.

    1980-01-01

    It is shown that if single particle currents are coupled to Maxwell's equations, the resulting set of self-consistent nonlinear equations describes the evolution of the electron beam and the amplitude and phase of the free-electron-laser field. The formulation is based on the slowly varying amplitude and phase approximation, and the distinction between microscopic and macroscopic scales, which distinguishes the microscopic bunching from the macroscopic pulse propagation. The capabilities of this new theoretical approach become apparent when its predictions for the ultrashort pulse free-electron laser are compared to experimental data; the optical pulse evolution, determined simply and accurately, agrees well with observations.

  19. Uncovering non-ergodicity on the cell membrane using single particle tracking approaches

    OpenAIRE

    Symeonidou Besi, Parthena

    2013-01-01

    Treball final de màster oficial fet en col·laboració amb Universitat Autònoma de Barcelona (UAB), Universitat de Barcelona (UB) i Institut de Ciències Fotòniques (ICFO) [ANGLES] In this work, we study the diffusion on the plasma membrane of the receptor DC-SIGN. The data we used were obtained by Single Particle Tracking technique and hence consist of individual trajectories. Motivated by investigating the dynamics of this receptor, our analysis comprises not only of standard statistical ap...

  20. Effects of single particle on shape phase transitions and phase coexistence in odd-even nuclei

    Science.gov (United States)

    Yu, Xiang-Ru; Hu, Jing; Li, Xiao-Xue; An, Si-Yu; Zhang, Yu

    2018-02-01

    A classical analysis of shape phase transitions and phase coexistence in odd-even nuclei has been performed in the framework of the interacting boson-fermion model. The results indicate that the effects of a single particle may influence different types of transitions in different ways. Especially, it is revealed that phase coexistence can clearly emerge in the critical region and thus be taken as a indicator of the shape phase transitions in odd-even nuclei. Supported by National Natural Science Foundation of China (11375005)

  1. Single-particle spectral function of a generalized Hubbard model: Metal-insulator transition

    Science.gov (United States)

    Gagliano, E. R.; Aligia, A. A.; Arrachea, Liliana; Avignon, Michel

    1995-05-01

    A generalized Hubbard model with correlated hoppings is studied at half filling using exact diagonalization methods. For certain values of the hopping parameters our results for several static properties, the Drude weight and the single-particle spectra function, suggest the occurrence of a metal-insulator transition (MIT) at a finite value of the local Coulomb interaction Uc. We identify the regions of the hopping parameters where the MIT is of the Mott type. In these regions, for large U

  2. Structural defect induced peak splitting in gold-copper bimetallic nanorods during growth by single particle spectroscopy.

    Science.gov (United States)

    Thota, Sravan; Chen, Shutang; Zhou, Yadong; Zhang, Yong; Zou, Shengli; Zhao, Jing

    2015-09-21

    A single particle level study of bimetallic nanoparticle growth provides valuable information that is usually hidden in ensemble measurements, helping to improve the understanding of a reaction mechanism and overcome the synthetic challenges. In this study, we use single particle spectroscopy to monitor the changes in the scattering spectra of Au-Cu alloy nanorods during growth. We found that the unique features of the single particle scattering spectra were due to atomic level geometric defects in the nanorods. Electrodynamics simulations have demonstrated that small structural defects of a few atomic layers split the scattering peaks, giving rise to higher order modes, which do not exist in defect-free rods of similar geometry. The study shows that single particle scattering technique is as sensitive as high-resolution electron microscopy in revealing atomic level structural defects.

  3. Massively parallel unsupervised single-particle cryo-EM data clustering via statistical manifold learning.

    Science.gov (United States)

    Wu, Jiayi; Ma, Yong-Bei; Congdon, Charles; Brett, Bevin; Chen, Shuobing; Xu, Yaofang; Ouyang, Qi; Mao, Youdong

    2017-01-01

    Structural heterogeneity in single-particle cryo-electron microscopy (cryo-EM) data represents a major challenge for high-resolution structure determination. Unsupervised classification may serve as the first step in the assessment of structural heterogeneity. However, traditional algorithms for unsupervised classification, such as K-means clustering and maximum likelihood optimization, may classify images into wrong classes with decreasing signal-to-noise-ratio (SNR) in the image data, yet demand increased computational costs. Overcoming these limitations requires further development of clustering algorithms for high-performance cryo-EM data processing. Here we introduce an unsupervised single-particle clustering algorithm derived from a statistical manifold learning framework called generative topographic mapping (GTM). We show that unsupervised GTM clustering improves classification accuracy by about 40% in the absence of input references for data with lower SNRs. Applications to several experimental datasets suggest that our algorithm can detect subtle structural differences among classes via a hierarchical clustering strategy. After code optimization over a high-performance computing (HPC) environment, our software implementation was able to generate thousands of reference-free class averages within hours in a massively parallel fashion, which allows a significant improvement on ab initio 3D reconstruction and assists in the computational purification of homogeneous datasets for high-resolution visualization.

  4. Shape evolution and single particle luminescence of organometal halide perovskite nanocrystals.

    Science.gov (United States)

    Zhu, Feng; Men, Long; Guo, Yijun; Zhu, Qiaochu; Bhattacharjee, Ujjal; Goodwin, Peter M; Petrich, Jacob W; Smith, Emily A; Vela, Javier

    2015-03-24

    Organometallic halide perovskites CH3NH3PbX3 (X = I, Br, Cl) have quickly become one of the most promising semiconductors for solar cells, with photovoltaics made of these materials reaching power conversion efficiencies of near 20%. Improving our ability to harness the full potential of organometal halide perovskites will require more controllable syntheses that permit a detailed understanding of their fundamental chemistry and photophysics. In this manuscript, we systematically synthesize CH3NH3PbX3 (X = I, Br) nanocrystals with different morphologies (dots, rods, plates or sheets) by using different solvents and capping ligands. CH3NH3PbX3 nanowires and nanorods capped with octylammonium halides show relatively higher photoluminescence (PL) quantum yields and long PL lifetimes. CH3NH3PbI3 nanowires monitored at the single particle level show shape-correlated PL emission across whole particles, with little photobleaching observed and very few off periods. This work highlights the potential of low-dimensional organometal halide perovskite semiconductors in constructing new porous and nanostructured solar cell architectures, as well as in applying these materials to other fields such as light-emitting devices and single particle imaging and tracking.

  5. Bidirectional reflectance spectroscopy 7. The single particle phase function hockey stick relation

    Science.gov (United States)

    Hapke, Bruce

    2012-11-01

    The measured volume-average single particle angular scattering functions of a large number of types of particle of interest for planetary regoliths in the visible-near-IR wavelength region can be represented to a reasonable approximation by two-parameter, double Henyey-Greenstein functions. When the two parameters of this function are plotted against one another they are found to be inversely correlated and lie within a restricted zone shaped like a hockey stick within the parameter space. The centroid of the zone is a curve that can be represented by a simple empirical equation. The wide variety of types of particles used to construct the plot implies that this equation may represent most of the particles found in regoliths. This means that when modeling the bidirectional reflectance of a regolith it may be possible to reduce the number of parameters necessary to specify the reflectance, and also to characterize the entire single particle phase function from observations at phase angles less than 90°. Even if the hockey stick relation has a finite width, rather than being a line, it restricts the parameter space that must be searched when fitting data. The curve should also be useful for forward modeling particle phase functions.

  6. Study on the fragmentation of granite due to the impact of single particle and double particles

    Directory of Open Access Journals (Sweden)

    Yuchun Kuang

    2016-09-01

    Full Text Available Particle Impact Drilling (PID is a novel method to improve the rate of penetration (ROP. In order to further improve the performance of PID, an investigation into the effect of single and double particles: (1 diameter; (2 initial velocity; (3 distance; and (4 angle of incidence was undertaken to investigate their effects on broken volume and penetration depth into hard brittle rock. For this purpose, the laboratory experiment of single particle impact rock was employed. Meanwhile, based on the LS-DYNA, a new finite element (FE simulation of the PID, including single and double particles impact rock, has been presented. The 3-dimensional (3D, aix-symmetric, dynamic-explicit, Lagrangian model has been considered in this simulation. And the Elastic and Holmquist Johnson Cook (HJC material behaviors have been used for particles and rocks, respectively. The FE simulation results of single particle impacting rock are good agreement with experimental data. Furthermore, in this article the optimal impact parameters, including diameter, initial velocity, distance and the angle of incidence, are obtained in PID.

  7. Single Particle Cryo-electron Microscopy and 3-D Reconstruction of Viruses

    Science.gov (United States)

    Guo, Fei; Jiang, Wen

    2014-01-01

    With fast progresses in instrumentation, image processing algorithms, and computational resources, single particle electron cryo-microscopy (cryo-EM) 3-D reconstruction of icosahedral viruses has now reached near-atomic resolutions (3–4 Å). With comparable resolutions and more predictable outcomes, cryo-EM is now considered a preferred method over X-ray crystallography for determination of atomic structure of icosahedral viruses. At near-atomic resolutions, all-atom models or backbone models can be reliably built that allow residue level understanding of viral assembly and conformational changes among different stages of viral life cycle. With the developments of asymmetric reconstruction, it is now possible to visualize the complete structure of a complex virus with not only its icosahedral shell but also its multiple non-icosahedral structural features. In this chapter, we will describe single particle cryo-EM experimental and computational procedures for both near-atomic resolution reconstruction of icosahedral viruses and asymmetric reconstruction of viruses with both icosahedral and non-icosahedral structure components. Procedures for rigorous validation of the reconstructions and resolution evaluations using truly independent de novo initial models and refinements are also introduced. PMID:24357374

  8. Mask-based approach to phasing of single-particle diffraction data.

    Science.gov (United States)

    Lunin, Vladimir Y; Lunina, Natalia L; Petrova, Tatiana E; Baumstark, Manfred W; Urzhumtsev, Alexandre G

    2016-01-01

    A Monte Carlo-type approach for low- and medium-resolution phasing of single-particle diffraction data is suggested. Firstly, the single-particle phase problem is substituted with the phase problem for an imaginary crystal. A unit cell of this crystal contains a single isolated particle surrounded by a large volume of bulk solvent. The developed phasing procedure then generates a large number of connected and finite molecular masks, calculates their Fourier coefficients, selects the sets with magnitudes that are highly correlated with the experimental values and finally aligns the selected phase sets and calculates the averaged phase values. A test with the known structure of monomeric photosystem II resulted in phases that have 97% correlation with the exact phases in the full 25 Å resolution shell (1054 structure factors) and correlations of 99, 94, 81 and 79% for the resolution shells ∞-60, 60-40, 40-30 and 30-25 Å, respectively. The same procedure may be used for crystallographic ab initio phasing.

  9. Silver nanoflowers for single-particle SERS with 10 pM sensitivity.

    Science.gov (United States)

    Roy, Shrawan; Muhammed Ajmal, C; Baik, Seunghyun; Kim, Jeongyong

    2017-11-17

    Surface-enhanced Raman scattering (SERS) has received considerable attention as a noninvasive optical sensing technique with ultrahigh sensitivity. While numerous types of metallic particles have been actively investigated as SERS substrates, the development of new SERS agents with high sensitivity and their reliable characterization are still required. Here we report the preparation and characterization of flower-shaped silver (Ag) nanoparticles that exhibit high-sensitivity single-particle SERS performance. Ag nanoflowers (NFs) with bud sizes in the range 220-620 nm were synthesized by the wet synthesis method. The densely packed nanoscale petals with thicknesses in the range 9-22 nm exhibit a large number of hot spots that significantly enhance their plasmonic activity. A single Ag NF particle (530-620 nm) can detect as little as 10 -11 M 4-mercaptobenzoic acid, and thus provides a sensitivity three orders of SERS magnitude greater than that of a spherical Ag nanoparticle. The analytical enhancement factors for single Ag NF particles were found to be as high as 8.0 × 10 9 , providing unprecedented high SERS detectivity at the single particle level. Here we present an unambiguous and systematic assessment of the SERS performances of the Ag NFs and demonstrate that they provide highly sensitive sensing platforms by single SERS particle.

  10. Detection of isolated protein-bound metal ions by single-particle cryo-STEM.

    Science.gov (United States)

    Elad, Nadav; Bellapadrona, Giuliano; Houben, Lothar; Sagi, Irit; Elbaum, Michael

    2017-10-17

    Metal ions play essential roles in many aspects of biological chemistry. Detecting their presence and location in proteins and cells is important for understanding biological function. Conventional structural methods such as X-ray crystallography and cryo-transmission electron microscopy can identify metal atoms on protein only if the protein structure is solved to atomic resolution. We demonstrate here the detection of isolated atoms of Zn and Fe on ferritin, using cryogenic annular dark-field scanning transmission electron microscopy (cryo-STEM) coupled with single-particle 3D reconstructions. Zn atoms are found in a pattern that matches precisely their location at the ferroxidase sites determined earlier by X-ray crystallography. By contrast, the Fe distribution is smeared along an arc corresponding to the proposed path from the ferroxidase sites to the mineral nucleation sites along the twofold axes. In this case the single-particle reconstruction is interpreted as a probability distribution function based on the average of individual locations. These results establish conditions for detection of isolated metal atoms in the broader context of electron cryo-microscopy and tomography.

  11. Massively parallel unsupervised single-particle cryo-EM data clustering via statistical manifold learning

    Science.gov (United States)

    Wu, Jiayi; Ma, Yong-Bei; Congdon, Charles; Brett, Bevin; Chen, Shuobing; Xu, Yaofang; Ouyang, Qi

    2017-01-01

    Structural heterogeneity in single-particle cryo-electron microscopy (cryo-EM) data represents a major challenge for high-resolution structure determination. Unsupervised classification may serve as the first step in the assessment of structural heterogeneity. However, traditional algorithms for unsupervised classification, such as K-means clustering and maximum likelihood optimization, may classify images into wrong classes with decreasing signal-to-noise-ratio (SNR) in the image data, yet demand increased computational costs. Overcoming these limitations requires further development of clustering algorithms for high-performance cryo-EM data processing. Here we introduce an unsupervised single-particle clustering algorithm derived from a statistical manifold learning framework called generative topographic mapping (GTM). We show that unsupervised GTM clustering improves classification accuracy by about 40% in the absence of input references for data with lower SNRs. Applications to several experimental datasets suggest that our algorithm can detect subtle structural differences among classes via a hierarchical clustering strategy. After code optimization over a high-performance computing (HPC) environment, our software implementation was able to generate thousands of reference-free class averages within hours in a massively parallel fashion, which allows a significant improvement on ab initio 3D reconstruction and assists in the computational purification of homogeneous datasets for high-resolution visualization. PMID:28786986

  12. Fourier transforms of single-particle wave functions in cylindrical coordinates

    International Nuclear Information System (INIS)

    Rizea, M.; Carjan, N.

    2016-01-01

    A formalism and the corresponding numerical procedures that calculate the Fourier transform of a single-particle wave function defined on a grid of cylindrical (ρ, z) coordinates is presented. Single-particle states in spherical and deformed nuclei have been chosen in view of future applications in the field of nuclear reactions. Bidimensional plots of the probability that the nucleon's momentum has a given value K = √(k ρ 2 +k z 2 ) are produced and from them the K -distributions are deduced. Three potentials have been investigated: (a) a sharp surface spherical well (i.e., of constant depth), (b) a spherical Woods-Saxon potential (i.e., diffuse surface) and (c) a deformed potential of Woods-Saxon type. In the first case the momenta are as well defined as allowed by the uncertainty principle. Depending on the state, their distributions have up to three separated peaks as a consequence of the up to three circular ridges of the bidimensional probabilities plots. In the second case the diffuseness allows very low momenta to be always populated thus creating tails towards the origin (K = 0). The peaks are still present but not well separated. In the third case the deformation transforms the above mentioned circular ridges into ellipses thus spreading the K-values along them. As a consequence the K-distributions have only one broad peak. (orig.)

  13. Recognition and separation of single particles with size variation by statistical analysis of their images.

    Science.gov (United States)

    White, Helen E; Saibil, Helen R; Ignatiou, Athanasios; Orlova, Elena V

    2004-02-13

    Macromolecules may occupy conformations with structural differences that cannot be resolved biochemically. The separation of mixed molecular populations is a pressing problem in single-particle analysis. Until recently, the task of distinguishing small structural variations was intractable, but developments in cryo-electron microscopy hardware and software now make it possible to address this problem. We have developed a general strategy for recognizing and separating structures of variable size from cryo-electron micrographs of single particles. The method uses a combination of statistical analysis and projection matching to multiple models. Identification of size variations by multivariate statistical analysis was used to do an initial separation of the data and generate starting models by angular reconstitution. Refinement was performed using alternate projection matching to models and angular reconstitution of the separated subsets. The approach has been successful at intermediate resolution, taking it within range of resolving secondary structure elements of proteins. Analysis of simulated and real data sets is used to illustrate the problems encountered and possible solutions. The strategy developed was used to resolve the structures of two forms of a small heat shock protein (Hsp26) that vary slightly in diameter and subunit packing.

  14. Low cost, high performance processing of single particle cryo-electron microscopy data in the cloud.

    Science.gov (United States)

    Cianfrocco, Michael A; Leschziner, Andres E

    2015-05-08

    The advent of a new generation of electron microscopes and direct electron detectors has realized the potential of single particle cryo-electron microscopy (cryo-EM) as a technique to generate high-resolution structures. Calculating these structures requires high performance computing clusters, a resource that may be limiting to many likely cryo-EM users. To address this limitation and facilitate the spread of cryo-EM, we developed a publicly available 'off-the-shelf' computing environment on Amazon's elastic cloud computing infrastructure. This environment provides users with single particle cryo-EM software packages and the ability to create computing clusters with 16-480+ CPUs. We tested our computing environment using a publicly available 80S yeast ribosome dataset and estimate that laboratories could determine high-resolution cryo-EM structures for $50 to $1500 per structure within a timeframe comparable to local clusters. Our analysis shows that Amazon's cloud computing environment may offer a viable computing environment for cryo-EM.

  15. Single-Particle Cryo-EM of the Ryanodine Receptor Channel in an Aqueous Environment.

    Science.gov (United States)

    Baker, Mariah R; Fan, Guizhen; Serysheva, Irina I

    2015-01-07

    Ryanodine receptors (RyRs) are tetrameric ligand-gated Ca(2+) release channels that are responsible for the increase of cytosolic Ca(2+) concentration leading to muscle contraction. Our current understanding of RyR channel gating and regulation is greatly limited due to the lack of a high-resolution structure of the channel protein. The enormous size and unwieldy shape of Ca(2+) release channels make X-ray or NMR methods difficult to apply for high-resolution structural analysis of the full-length functional channel. Single-particle electron cryo-microscopy (cryo-EM) is one of the only effective techniques for the study of such a large integral membrane protein and its molecular interactions. Despite recent developments in cryo-EM technologies and break-through single-particle cryo-EM studies of ion channels, cryospecimen preparation, particularly the presence of detergent in the buffer, remains the main impediment to obtaining atomic-resolution structures of ion channels and a multitude of other integral membrane protein complexes. In this review we will discuss properties of several detergents that have been successfully utilized in cryo-EM studies of ion channels and the emergence of the detergent alternative amphipol to stabilize ion channels for structure-function characterization. Future structural studies of challenging specimen like ion channels are likely to be facilitated by cryo-EM amenable detergents or alternative surfactants.

  16. Massively parallel unsupervised single-particle cryo-EM data clustering via statistical manifold learning.

    Directory of Open Access Journals (Sweden)

    Jiayi Wu

    Full Text Available Structural heterogeneity in single-particle cryo-electron microscopy (cryo-EM data represents a major challenge for high-resolution structure determination. Unsupervised classification may serve as the first step in the assessment of structural heterogeneity. However, traditional algorithms for unsupervised classification, such as K-means clustering and maximum likelihood optimization, may classify images into wrong classes with decreasing signal-to-noise-ratio (SNR in the image data, yet demand increased computational costs. Overcoming these limitations requires further development of clustering algorithms for high-performance cryo-EM data processing. Here we introduce an unsupervised single-particle clustering algorithm derived from a statistical manifold learning framework called generative topographic mapping (GTM. We show that unsupervised GTM clustering improves classification accuracy by about 40% in the absence of input references for data with lower SNRs. Applications to several experimental datasets suggest that our algorithm can detect subtle structural differences among classes via a hierarchical clustering strategy. After code optimization over a high-performance computing (HPC environment, our software implementation was able to generate thousands of reference-free class averages within hours in a massively parallel fashion, which allows a significant improvement on ab initio 3D reconstruction and assists in the computational purification of homogeneous datasets for high-resolution visualization.

  17. Evolution of Single Particle and Collective properties in the Neutron-Rich Mg Isotopes

    CERN Multimedia

    Reiter, P; Wiens, A; Fitting, J; Lauer, M; Van duppen, P L E; Finke, F

    2002-01-01

    We propose to study the single particle and collective properties of the neutron-rich Mg isotopes in transfer reactions and Coulomb excitation using REX-ISOLDE and MINIBALL. From the Coulomb excitation measurement precise and largely model independent B( E2 ; 0$^{+}_{g.s.}\\rightarrow$ 2$^{+}_{1}$ ) will be determined for the even-even isotopes. For the odd isotopes the distribution of the E2 strength over a few low-lying states will be measured. The sign of the M1/E2 mixing ratio, extracted from angular distributions, is characteristic of the sign of the deformation, as is the resulting level scheme. The neutron-pickup channel in the transfer reactions will allow for a determination of the single particle properties (spin, parity, spectroscopic factors) of these nuclei. This information will give new insights in changes of nuclear structure in the vicinity of the island of deformation around $^{32}$Mg. A total of 24 shifts of REX beam time is requested.

  18. The development of optical microscopy techniques for the advancement of single-particle studies

    Energy Technology Data Exchange (ETDEWEB)

    Marchuk, Kyle [Iowa State Univ., Ames, IA (United States)

    2013-05-15

    Single particle orientation and rotational tracking (SPORT) has recently become a powerful optical microscopy tool that can expose many molecular motions. Unfortunately, there is not yet a single microscopy technique that can decipher all particle motions in all environmental conditions, thus there are limitations to current technologies. Within, the two powerful microscopy tools of total internal reflection and interferometry are advanced to determine the position, orientation, and optical properties of metallic nanoparticles in a variety of environments. Total internal reflection is an optical phenomenon that has been applied to microscopy to produce either fluorescent or scattered light. The non-invasive far-field imaging technique is coupled with a near-field illumination scheme that allows for better axial resolution than confocal microscopy and epi-fluorescence microscopy. By controlling the incident illumination angle using total internal reflection fluorescence (TIRF) microscopy, a new type of imaging probe called “non-blinking” quantum dots (NBQDs) were super-localized in the axial direction to sub-10-nm precision. These particles were also used to study the rotational motion of microtubules being propelled by the motor protein kinesin across the substrate surface. The same instrument was modified to function under total internal reflection scattering (TIRS) microscopy to study metallic anisotropic nanoparticles and their dynamic interactions with synthetic lipid bilayers. Utilizing two illumination lasers with opposite polarization directions at wavelengths corresponding to the short and long axis surface plasmon resonance (SPR) of the nanoparticles, both the in-plane and out-of-plane movements of many particles could be tracked simultaneously. When combined with Gaussian point spread function (PSF) fitting for particle super-localization, the binding status and rotational movement could be resolved without degeneracy. TIRS microscopy was also used to

  19. Biomass Burning Research Using DOE ARM Single-Particle Soot Photometer (SP2) Field Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    Onasch, Timothy B [Aerodyne Research, Inc., Billerica, MA (United States); Sedlacek, Arthur J [Brookhaven National Lab. (BNL), Upton, NY (United States); Lewis, Ernie [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2017-03-01

    The focus of this laboratory study was to investigate the chemical and optical properties, and the detection efficiencies, of tar balls generated in the laboratory using the same instruments deployed on the U.S. Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) Climate Research Facility Gulfstream-1 (G-1) aircraft during the 2013 Biomass Burning Observation Project (BBOP) field study, during which tar balls were observed in wildland biomass burning particulate emissions. Key goals of this laboratory study were: (a) measuring the chemical composition of tar balls to provide insights into the atmospheric processes that form (evaporation/oxidation) and modify them in biomass burning plumes, (b) identifying whether tar balls contain refractory black carbon, (c) determining the collection efficiencies of tar balls impacting on the 600oC heated tungsten vaporizer in the Aerodyne Soot Particle Aerosol Mass Spectrometer (SP-AMS) (i.e., given the observed low volatilities, AMS measurements might underestimate organic biomass burning plume loadings), and (d) measuring the wavelength-dependent, mass-specific absorption cross-sections of brown carbon components of tar balls. This project was funded primarily by the DOE Atmospheric System Research (ASR) program, and the ARM Facility made their single-particle soot photometer (SP2) available for September 1-September 31, 2016 in the Aerodyne laboratories. The ARM mentor (Dr. Sedlacek) requested no funds for mentorship or data reduction. All ARM SP2 data collected as part of this project are archived in the ARM Data Archive in accordance with established protocols. The main objectives of the ARM Biomass Burning Observation Period (BBOP, July-October, 2013) field campaign were to (1) assess the impact of wildland fires in the Pacific Northwest on climate, through near-field and regional intensive measurement campaigns, and (2) investigate agricultural burns to determine how those biomass burn plumes differ from

  20. [Analysis of Single Particle Aging and Mixing State at an Agriculture Site (Quzhou) in the North China Plain in Summer Using a Single Particle Aerosol Mass Spectrometer].

    Science.gov (United States)

    Huang, Zi-long; Zeng, Li-mm; Dong, I-Iua-Bin; Li, Mei; Zhu, Tong

    2016-04-15

    To characterize the size distribution and chemical ompsitins f abiet prtices t a agicuturesit intheNorh o Chinese Plain, a single particle aerosol mass spectrometer (SPAMS) was deployed from June 30 to July 8, 2013. A total of 230,152 particles in the size range of 0.2-2.0 pm were chemically analyzed with both positive and negative ion spectra. The results revealed that aerosol could he classified into eight dominant groups, including elemental carbon (EC, 55.5%), organic carbon (OC, 10.7%), alkalis (Na-K, 17.4%), other metals (1.7%), Fe-rich (6.3%), Pb-rich (3.1%), dust (4.8%), and other (0.8%). The observed eight types of particles contained secondary components such as 46NO2-, 62NO3-, 96SO3-, 96SO4-, 97HSO4-, showing that they probably went through different aging processes. The analysis of particle size distribution showed that 700-800 nm was the peak value of all particles, and that dust and Fe particles were mainly in the coarse size range. EC particles subtype group research revealed EC particles tended to be aging with the above mentioned secondary ions and eventually led to a particle type conversion from EC to the less aging ECN and the more serious aging ECS, the diurnal variation of which was obviously negatively correlated, and there was a possibility of forming OC/EC mixture with the adsorption of secondary organic matter on EC surface.

  1. Investigation of single-mode and multi-mode hydromagnetic Rayleigh-Taylor instability in planar geometry

    International Nuclear Information System (INIS)

    Roderick, N.F.; Cochrane, K.; Douglas, M.R.

    1998-01-01

    Previous investigations carried out to study various methods of seeding the hydromagnetic Rayleigh-Taylor instability in magnetohydrodynamic simulations showed features similar to those seen in hydrodynamic calculations. For periodic single-mode initiations the results showed the appearance of harmonics as the single modes became nonlinear. For periodic multi-mode initiations new modes developed that indicated the presence of mode coupling. The MHD simulations used parameters of the high velocity large radius z-pinch experiments performed in the Z-accelerator at Sandia National Laboratories. The cylindrical convergent geometry and variable acceleration of these configurations made comparison with analytic, developed for planar geometry with constant acceleration, difficult. A set of calculations in planar geometry using constant current to produce acceleration and parameters characteristic of the cylindrical implosions has been performed to allow a better comparison. Results of these calculations, comparison with analytic theory, and comparison with the cylindrical configuration calculations will be discussed

  2. Dynamic configuration management of a multi-standard and multi-mode reconfigurable multi-ASIP architecture for turbo decoding

    Science.gov (United States)

    Lapotre, Vianney; Gogniat, Guy; Baghdadi, Amer; Diguet, Jean-Philippe

    2017-12-01

    The multiplication of connected devices goes along with a large variety of applications and traffic types needing diverse requirements. Accompanying this connectivity evolution, the last years have seen considerable evolutions of wireless communication standards in the domain of mobile telephone networks, local/wide wireless area networks, and Digital Video Broadcasting (DVB). In this context, intensive research has been conducted to provide flexible turbo decoder targeting high throughput, multi-mode, multi-standard, and power consumption efficiency. However, flexible turbo decoder implementations have not often considered dynamic reconfiguration issues in this context that requires high speed configuration switching. Starting from this assessment, this paper proposes the first solution that allows frame-by-frame run-time configuration management of a multi-processor turbo decoder without compromising the decoding performances.

  3. Choosing a Better Delay Line Medium between Single-Mode and Multi-Mode Optical Fibers: the Effect of Bending

    Directory of Open Access Journals (Sweden)

    Auwal Mustapha Imam

    2017-12-01

    Full Text Available Optical fiber cables are materials whose core is made of silica and other materials such as chalcogenide glasses; they transmit a digital signal via light pulses through an extremely thin strand of glass. The light propagates and is being guided by the core which is surrounded by the cladding. Light travels in the optical fiber in the form of total internal reflection in the core of the fibers. The flexibility, low tensile strength, low signal loss, high bandwidth and other characteristics of optical fibers favors it for use as a delay medium in many applications. Another favorable characteristic of optical fiber delay lines is are their relative insensitivities to environmental effects and electromagnetic interferences. The immunity of optical fibers to interferences and their less weight added advantages to it for use as delay medium. Single-mode and multi-mode are the two most popular types of optical fibers. Single-mode fibers have good propagation and delay properties with a minimal loss that allows the signal to propagate in a large distance with insignificant distortion or attenuation. The percentage of power transmission of single-mode fibers is found to be higher than that of the multi-mode fibers. It is, therefore, a preferred type for use as a delay line. In this paper, relative studies of the two optical fibers modes, and the results of power input/output measurement of the two modes are presented with a view to coming up with a better type for use as a delay medium.

  4. Single particle characterization of black carbon aerosols at a tropospheric alpine site in Switzerland

    Science.gov (United States)

    Liu, D.; Flynn, M.; Gysel, M.; Targino, A.; Crawford, I.; Bower, K.; Choularton, T.; Jurányi, Z.; Steinbacher, M.; Hüglin, C.; Curtius, J.; Kampus, M.; Petzold, A.; Weingartner, E.; Baltensperger, U.; Coe, H.

    2010-08-01

    The refractory black carbon (rBC) mass, size distribution (190-720 nm) and mixing state in sub-micron aerosols were characterized from late February to March 2007 using a single particle incandescence method at the high alpine research station Jungfraujoch (JFJ), Switzerland (46.33° N, 7.59° E, 3580 m a.s.l.). JFJ is a ground based location, which is at times exposed to continental free tropospheric air. A median mass absorption coefficient (MAC) of 10.2±3.2 m2 g-1 at λ=630 nm was derived by comparing single particle incandescence measurements of black carbon mass with continuous measurements of absorption coefficient. This value is comparable with other estimates at this location. The aerosols measured at the site were mostly well mixed and aged during transportation via the free troposphere. Pollutant sources were traced by air mass back trajectories, trace gases concentrations and the mass loading of rBC. In southeasterly wind directions, mixed or convective weather types provided the potential to vent polluted boundary layer air from the southern Alpine area and industrial northern Italy, delivering enhanced rBC mass loading and CN concentrations to the JFJ. The aerosol loadings at this site were also significantly influenced by precipitation, which led to the removal of rBC from the atmosphere. Precipitation events were shown to remove about 65% of the rBC mass from the free tropospheric background reducing the mean loading from 13±5 ng m-3 to 6±2 ng m-3(corrected to standard temperature and pressure). Overall, 40±15% of the observed rBC particles within the detectable size range were mixed with large amounts of non-refractory materials present as a thick coating. The growth of particle size into the accumulation mode was positively linked with the degree of rBC mixing, suggesting the important role of condensable materials in increasing particle size and leading to enhanced internal mixing of these materials with rBC. It is the first time that BC mass

  5. Decay modes of high-lying single-particle states in [sup 209]Pb

    Energy Technology Data Exchange (ETDEWEB)

    Beaumel, D.; Fortier, S.; Gales, S.; Guillot, J.; Langevin-Joliot, H.; Laurent, H.; Maison, J.M.; Vernotte, J.; Bordewijk, J.A.; Brandenburg, S.; Krasznahorkay, A.; Crawley, G.M.; Massolo, C.P.; Renteria, M. (Institut de Physique Nucleaire, Institut National de Physique Nucleaire et de Physique des Particules Centre National de la Recherche Scientifique, 91406 Orsay Cedex (France) Kernfysisch Versneller Instituut, 9747AA Groningen (Netherlands) National Superconducting Cyclotron Laboratory, Michigan State University, East Lansing, Michigan 48824 (United States) Departamento de Fisica, Fac. Cs. Exactas, Universidad Nacional de La Plata, CC No. 67, 1900 La Plata (Argentina))

    1994-05-01

    The neutron decay of high-lying single-particle states in [sup 209]Pb excited by means of the ([alpha],[sup 3]He) reaction has been investigated at 122 MeV incident energy using a multidetector array. The high spin values of these states, inferred from previous inclusive experiments, are confirmed by the present data involving angular correlation measurements and the determination of branching ratios to low lying levels in [sup 208]Pb. The structure located between 8.5 and 12 MeV excitation energy in [sup 209]Pb displays large departures from a pure statistical decay with significant direct feeding of the low-lying collective states (3[sup [minus

  6. Decay modes of high-lying single-particle states in 209Pb

    International Nuclear Information System (INIS)

    Beaumel, D.; Fortier, S.; Gales, S.; Guillot, J.; Crawley, G.M.; Massolo, C.P.; Renteria, M.

    1993-01-01

    The neutron decay of high-lying single-particle states in 209 Pb excited by means of the (α, 3 He) reaction has been investigated at 122 MeV incident energy using the multidetector array EDEN. The high spin values of these states, inferred from previous inclusive experiments, are confirmed by the present data involving angular correlation measurements and the determination of branching ratios to low lying levels in 208 Pb. The structure located between 8.5 and 12 MeV excitation energy in 209 Pb displays large departures from a pure statistical decay with significant direct feeding of the low-lying collective states (3 - ,5 - ) of 208 Pb. At higher excitation energy up to 20 MeV, the measured neutron decay is in agreement with the predictions of the statistical model. (authors). 24 refs., 16 figs., 2 tabs

  7. A clustering approach to multireference alignment of single-particle projections in electron microscopy.

    Science.gov (United States)

    Sorzano, C O S; Bilbao-Castro, J R; Shkolnisky, Y; Alcorlo, M; Melero, R; Caffarena-Fernández, G; Li, M; Xu, G; Marabini, R; Carazo, J M

    2010-08-01

    Two-dimensional analysis of projections of single-particles acquired by an electron microscope is a useful tool to help identifying the different kinds of projections present in a dataset and their different projection directions. Such analysis is also useful to distinguish between different kinds of particles or different particle conformations. In this paper we introduce a new algorithm for performing two-dimensional multireference alignment and classification that is based on a Hierarchical clustering approach using correntropy (instead of the more traditional correlation) and a modified criterion for the definition of the clusters specially suited for cases in which the Signal-to-Noise Ratio of the differences between classes is low. We show that our algorithm offers an improved sensitivity over current methods in use for distinguishing between different projection orientations and different particle conformations. This algorithm is publicly available through the software package Xmipp. Copyright 2010 Elsevier Inc. All rights reserved.

  8. Single-particle structure determination by correlations of snapshot X-ray diffraction patterns

    Science.gov (United States)

    Starodub, D.; Aquila, A.; Bajt, S.; Barthelmess, M.; Barty, A.; Bostedt, C.; Bozek, J. D.; Coppola, N.; Doak, R. B.; Epp, S. W.; Erk, B.; Foucar, L.; Gumprecht, L.; Hampton, C. Y.; Hartmann, A.; Hartmann, R.; Holl, P.; Kassemeyer, S.; Kimmel, N.; Laksmono, H.; Liang, M.; Loh, N. D.; Lomb, L.; Martin, A. V.; Nass, K.; Reich, C.; Rolles, D.; Rudek, B.; Rudenko, A.; Schulz, J.; Shoeman, R. L.; Sierra, R. G.; Soltau, H.; Steinbrener, J.; Stellato, F.; Stern, S.; Weidenspointner, G.; Frank, M.; Ullrich, J.; Strüder, L.; Schlichting, I.; Chapman, H. N.; Spence, J. C. H.; Bogan, M. J.

    2012-12-01

    Diffractive imaging with free-electron lasers allows structure determination from ensembles of weakly scattering identical nanoparticles. The ultra-short, ultra-bright X-ray pulses provide snapshots of the randomly oriented particles frozen in time, and terminate before the onset of structural damage. As signal strength diminishes for small particles, the synthesis of a three-dimensional diffraction volume requires simultaneous involvement of all data. Here we report the first application of a three-dimensional spatial frequency correlation analysis to carry out this synthesis from noisy single-particle femtosecond X-ray diffraction patterns of nearly identical samples in random and unknown orientations, collected at the Linac Coherent Light Source. Our demonstration uses unsupported test particles created via aerosol self-assembly, and composed of two polystyrene spheres of equal diameter. The correlation analysis avoids the need for orientation determination entirely. This method may be applied to the structural determination of biological macromolecules in solution.

  9. Single-particle And Collective Effects Of Cubic Nonlinearity In The Beam Dynamics Of Proton Synchrotrons

    CERN Document Server

    Tran Hy, J

    1998-01-01

    This thesis describes some new studies of the effects of cubic nonlinearities arising from image-charge forces and octupole magnets on the transverse beam dynamics of proton synchrotrons and storage rings, and also a study of the damping of coherent oscillations using a feed-back damper. In the latter case, various corrective algorithms were modeled using linear one-turn maps. Kicks of fixed amplitude but appropriate sign were shown to provide linear damping and no coherent tune shift, though the rate predicted analytically was somewhat higher than that observed in simulations. This algorithm gave much faster damping (for equal power) than conventional proportional kicks, which damp exponentially. Two single-particle effects of the image-change force were investigated: distortion of the momentum dispersion function and amplitude dependence of the betatron tunes (resulting in tune spread). The former is calculated using transfer maps and the method of undetermined coefficients, the latter by solving the cubic ...

  10. Response of microchannel plates in ionization mode to single particles and electromagnetic showers

    Science.gov (United States)

    Barnyakov, A. Yu.; Barnyakov, M. Yu.; Brianza, L.; Cavallari, F.; Cipriani, M.; Ciriolo, V.; del Re, D.; Gelli, S.; Ghezzi, A.; Gotti, C.; Govoni, P.; Katcin, A. A.; Malberti, M.; Martelli, A.; Marzocchi, B.; Meridiani, P.; Organtini, G.; Paramatti, R.; Pigazzini, S.; Preiato, F.; Prisekin, V. G.; Rahatlou, S.; Rovelli, C.; Santanastasio, F.; Tabarelli de Fatis, T.

    2018-01-01

    Hundreds of concurrent collisions per bunch crossing are expected at future hadron colliders. Precision timing calorimetry has been advocated as a way to mitigate the pileup effects and, thanks to their excellent time resolution, microchannel plates (MCPs) are good candidate detectors for this goal. We report on the response of MCPs, used as secondary emission detectors, to single relativistic particles and to electromagnetic showers. Several prototypes, with different geometries and characteristics, were exposed to particle beams at the INFN-LNF Beam Test Facility and at CERN. Their time resolution and efficiency are measured for single particles and as a function of the multiplicity of particles. Efficiencies between 50% and 90% to single relativistic particles are reached, and up to 100% in presence of a large number of particles. Time resolutions between 20 ps and 30 ps are obtained.

  11. Single particle transfer for quantitative analysis with total-reflection X-ray fluorescence spectrometry

    International Nuclear Information System (INIS)

    Esaka, Fumitaka; Esaka, Konomi T.; Magara, Masaaki; Sakurai, Satoshi; Usuda, Shigekazu; Watanabe, Kazuo

    2006-01-01

    The technique of single particle transfer was applied to quantitative analysis with total-reflection X-ray fluorescence (TXRF) spectrometry. The technique was evaluated by performing quantitative analysis of individual Cu particles with diameters between 3.9 and 13.2 μm. The direct quantitative analysis of the Cu particle transferred onto a Si carrier gave a discrepancy between measured and calculated Cu amounts due to the absorption effects of incident and fluorescent X-rays within the particle. By the correction for the absorption effects, the Cu amounts in individual particles could be determined with the deviation within 10.5%. When the Cu particles were dissolved with HNO 3 solution prior to the TXRF analysis, the deviation was improved to be within 3.8%. In this case, no correction for the absorption effects was needed for quantification

  12. Detecting the shape of anisotropic gold nanoparticles in dispersion with single particle extinction and scattering.

    Science.gov (United States)

    Potenza, M A C; Krpetić, Ž; Sanvito, T; Cai, Q; Monopoli, M; de Araújo, J M; Cella, C; Boselli, L; Castagnola, V; Milani, P; Dawson, K A

    2017-02-23

    The shape and size of nanoparticles are important parameters affecting their biodistribution, bioactivity, and toxicity. The high-throughput characterisation of the nanoparticle shape in dispersion is a fundamental prerequisite for realistic in vitro and in vivo evaluation, however, with routinely available bench-top optical characterisation techniques, it remains a challenging task. Herein, we demonstrate the efficacy of a single particle extinction and scattering (SPES) technique for the in situ detection of the shape of nanoparticles in dispersion, applied to a small library of anisotropic gold particles, with a potential development for in-line detection. The use of SPES paves the way to the routine quantitative analysis of nanoparticles dispersed in biologically relevant fluids, which is of importance for the nanosafety assessment and any in vitro and in vivo administration of nanomaterials.

  13. EMHP: an accurate automated hole masking algorithm for single-particle cryo-EM image processing.

    Science.gov (United States)

    Berndsen, Zachary; Bowman, Charles; Jang, Haerin; Ward, Andrew B

    2017-12-01

    The Electron Microscopy Hole Punch (EMHP) is a streamlined suite of tools for quick assessment, sorting and hole masking of electron micrographs. With recent advances in single-particle electron cryo-microscopy (cryo-EM) data processing allowing for the rapid determination of protein structures using a smaller computational footprint, we saw the need for a fast and simple tool for data pre-processing that could run independent of existing high-performance computing (HPC) infrastructures. EMHP provides a data preprocessing platform in a small package that requires minimal python dependencies to function. https://www.bitbucket.org/chazbot/emhp Apache 2.0 License. bowman@scripps.edu. Supplementary data are available at Bioinformatics online. © The Author(s) 2017. Published by Oxford University Press.

  14. An efficient, movable single-particle detector for use in cryogenic ultra-high vacuum environments.

    Science.gov (United States)

    Spruck, Kaija; Becker, Arno; Fellenberger, Florian; Grieser, Manfred; von Hahn, Robert; Klinkhamer, Vincent; Novotný, Oldřich; Schippers, Stefan; Vogel, Stephen; Wolf, Andreas; Krantz, Claude

    2015-02-01

    A compact, highly efficient single-particle counting detector for ions of keV/u kinetic energy, movable by a long-stroke mechanical translation stage, has been developed at the Max-Planck-Institut für Kernphysik (Max Planck Institute for Nuclear Physics, MPIK). Both, detector and translation mechanics, can operate at ambient temperatures down to ∼10 K and consist fully of ultra-high vacuum compatible, high-temperature bakeable, and non-magnetic materials. The set-up is designed to meet the technical demands of MPIK's Cryogenic Storage Ring. We present a series of functional tests that demonstrate full suitability for this application and characterise the set-up with regard to its particle detection efficiency.

  15. The on-line analysis of aerosol-delivered pharmaceuticals via single particle aerosol mass spectrometry.

    Science.gov (United States)

    Morrical, Bradley D; Balaxi, Maria; Fergenson, David

    2015-07-15

    The use of single particle aerosol mass spectrometry (SPAMS) was evaluated for the analysis of inhaled pharmaceuticals to determine the mass distribution of the individual active pharmaceutical ingredients (API) in both single ingredient and combination drug products. SPAMS is an analytical technique where the individual aerodynamic diameters and chemical compositions of many aerosol particles are determined in real-time. The analysis was performed using a Livermore Instruments SPAMS 3.0, which allowed the efficient analysis of aerosol particles with broad size distributions and can acquire data even under a very large particle load. Data similar to what would normally require roughly three days of experimentation and analysis was collected in a five minute period and analyzed automatically. The results were computed to be comparable to those returned by a typical Next Generation Impactor (NGI) particle size distribution experiment. Copyright © 2015. Published by Elsevier B.V.

  16. Sampling and single particle analysis for the chemical characterisation of fine atmospheric particulates: A review.

    Science.gov (United States)

    Elmes, Michele; Gasparon, Massimo

    2017-11-01

    To better understand the potential environmental and human health impacts of fine airborne particulate matter (APM), detailed physical and chemical characterisation is required. The only means to accurately distinguish between the multiple compositions in APM is by single particle analysis. A variety of methods and instruments are available, which range from filter-based sample collection for off-line laboratory analysis to on-line instruments that detect the airborne particles and generate size distribution and chemical data in real time. There are many reasons for sampling particulates in the ambient atmosphere and as a consequence, different measurement strategies and sampling devices are used depending on the scientific objectives and subsequent analytical techniques. This review is designed as a guide to some of the techniques available for the sampling and subsequent chemical analysis of individual inorganic particles. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Single particle momentum and angular distributions in hadron-hadron collisions at ultrahigh energies

    Science.gov (United States)

    Chou, T. T.; Chen, N. Y.

    1985-01-01

    The forward-backward charged multiplicity distribution (P n sub F, n sub B) of events in the 540 GeV antiproton-proton collider has been extensively studied by the UA5 Collaboration. It was pointed out that the distribution with respect to n = n sub F + n sub B satisfies approximate KNO scaling and that with respect to Z = n sub F - n sub B is binomial. The geometrical model of hadron-hadron collision interprets the large multiplicity fluctuation as due to the widely different nature of collisions at different impact parameters b. For a single impact parameter b, the collision in the geometrical model should exhibit stochastic behavior. This separation of the stochastic and nonstochastic (KNO) aspects of multiparticle production processes gives conceptually a lucid and attractive picture of such collisions, leading to the concept of partition temperature T sub p and the single particle momentum spectrum to be discussed in detail.

  18. 3D structure determination of protein using TEM single particle analysis.

    Science.gov (United States)

    Sato, Chikara; Mio, Kazuhiro; Kawata, Masaaki; Ogura, Toshihiko

    2014-11-01

    Proteins play important roles in cell functions such as enzymes, cell trafficking, neurotransmission, muscle contraction and hormone secretion. However, some proteins are very difficult to be crystallized and their structures are undetermined. Several techniques have been developed to elucidate the structure of macromolecules; X-ray or electron crystallography, nuclear magnetic resonance spectroscopy, and high-resolution electron microscopy. Among them, electron microscopy based single particle reconstruction (SPA) technique is a computer-aided structure determination method. This method reconstructs the 3D structure from projection images of dispersed protein. A large number of two-dimensional particle images are picked up from EM films, aligned and classified to generate 2D averages, and used to reconstruct the 3D structure by assigning the Euler angle of each 2D average. Due to the necessity of elaborate collaboration between the classical biology and the innovative information technology including parallel computing, scientists needed to break unseen barriers to get a start of this analysis. However, recent progresses in electron microscopes, mathematical algorithms, and computational abilities greatly reduced the height of barriers and expanded targets that are considered to be primarily addressable using single particle analysis. Membrane proteins are one of these targets to which the single particle analysis is successfully applied for the understanding of their 3D structures. For this purpose, we have developed various SPA methods [1-5] and applied them to different proteins [6-8].Here, we introduce reconstructed proteins, and discuss the availability of this technique. The intramembrane-cleaving proteases (I-CLiPs) that sever the transmembrane domains of their substrates have been identified in a range of organisms and play a variety of roles in biological conditions. I-CLiPs have been classified into three groups: serine-, aspartyl- and metalloprotease

  19. Proton resonance elastic scattering of $^{30}$Mg for single particle structure of $^{31}$Mg

    CERN Multimedia

    The single particle structure of $^{31}$Mg, which is located in the so-called “island of inversion”, will be studied through measuring Isobaric Analog Resonances (IARs) of bound states of $^{31}$Mg. They are located in the high excitation energy of $^{31}$Al. We are going to determine the spectroscopic factors and angular momenta of the parent states by measuring the excitation function of the proton resonance elastic scattering around 0 degrees in the laboratory frame with around 3 MeV/nucleon $^{30}$Mg beam. The present study will reveal the shell evolution around $^{32}$Mg. In addition, the spectroscopic factor of the (7/2)$^{−}$ state which was not yet determined experimentally, may allow one to study the shape coexistence in this nucleus.

  20. Singlet-triplet splittings from the virial theorem and single-particle excitation energies

    Science.gov (United States)

    Becke, Axel D.

    2018-01-01

    The zeroth-order (uncorrelated) singlet-triplet energy difference in single-particle excited configurations is 2Kif, where Kif is the Coulomb self-energy of the product of the transition orbitals. Here we present a non-empirical, virial-theorem argument that the correlated singlet-triplet energy difference should be half of this, namely, Kif. This incredibly simple result gives vertical HOMO-LUMO excitation energies in small-molecule benchmarks as good as the popular TD-B3LYP time-dependent approach to excited states. For linear acenes and nonlinear polycyclic aromatic hydrocarbons, the performance is significantly better than TD-B3LYP. In addition to the virial theorem, the derivation borrows intuitive pair-density concepts from density-functional theory.

  1. Estimating the anomalous diffusion exponent for single particle tracking data with measurement errors - An alternative approach.

    Science.gov (United States)

    Burnecki, Krzysztof; Kepten, Eldad; Garini, Yuval; Sikora, Grzegorz; Weron, Aleksander

    2015-06-11

    Accurately characterizing the anomalous diffusion of a tracer particle has become a central issue in biophysics. However, measurement errors raise difficulty in the characterization of single trajectories, which is usually performed through the time-averaged mean square displacement (TAMSD). In this paper, we study a fractionally integrated moving average (FIMA) process as an appropriate model for anomalous diffusion data with measurement errors. We compare FIMA and traditional TAMSD estimators for the anomalous diffusion exponent. The ability of the FIMA framework to characterize dynamics in a wide range of anomalous exponents and noise levels through the simulation of a toy model (fractional Brownian motion disturbed by Gaussian white noise) is discussed. Comparison to the TAMSD technique, shows that FIMA estimation is superior in many scenarios. This is expected to enable new measurement regimes for single particle tracking (SPT) experiments even in the presence of high measurement errors.

  2. Logarithmic Decay in Single-Particle Relaxation of Hydrated Lysozyme Powder

    Science.gov (United States)

    Lagi, Marco; Baglioni, Piero; Chen, Sow-Hsin

    2009-09-01

    We present the self-dynamics of protein amino acids of hydrated lysozyme powder around the physiological temperature by means of molecular dynamics simulations. The self-intermediate scattering functions of the amino acid residue center of mass display a logarithmic decay over 3 decades of time, from 2 ps to 2 ns, followed by an exponential α relaxation. This kind of slow dynamics resembles the relaxation scenario within the β-relaxation time range predicted by mode coupling theory in the vicinity of higher-order singularities. These results suggest a strong analogy between the single-particle dynamics of the protein and the dynamics of colloidal, polymeric, and molecular glass-forming liquids.

  3. Preliminary Understanding of Surface Plasmon-Enhanced Circular Dichroism Spectroscopy by Single Particle Imaging

    Science.gov (United States)

    Zhan, Kangshu

    Monitoring chiral optical signals of biomolecules as their conformation changes is an important means to study their structures, properties, and functions. Most measurements, however, are ensemble measurements because chiral optical signals from a single biomolecule is often too weak to be detected. In this dissertation, I present my early attempts to study conformational changes of adsorbed proteins by taking advantage of the enhanced electromagnetic (EM) field around a well-designed plasmonic nanofeature. In particular, I discuss the detection of protein adsorption and denaturation on metallic nanoparticles using single particle scattering and CD spectroscopic imaging. Particles of two distinctively different sizes were compared and two different sample protein molecules were studied. A combination of experimental and computational tools was used to simulate and interpret the collected scattering and CD results. The first chapter provides a brief overview of the state-of-art research in CD spectroscopic studies at the single particle level. Three different means to make particles capable of chiral detection are discussed. Various applications beyond single particle imaging are presented to showcase the potential of the described research project, beyond our immediate goals. The second chapter describes my initial characterization of large, metallic, anisotropic nanorods and the establishment of experimental procedures used later for spectrum reconstruction, data visualization and analysis. The physical shape and structure of the particles were imaged by scanning electron microscopy (SEM), the chemical composition by energy dispersive X-ray Spectroscopy (EDS), and the optical properties by darkfield microscopy. An experimental protocol was developed to connect information collected from separate techniques for the same particle, with the aims of discovering any possible structural-property correlation. The reproducibility of the single particle imaging method was

  4. Single particle analysis with a 360/sup 0/ light scattering photometer

    Energy Technology Data Exchange (ETDEWEB)

    Bartholdi, M.F.

    1979-06-01

    Light scattering by single spherical homogeneous particles in the diameter range 1 to 20 ..mu..m and relative refractive index 1.20 is measured. Particle size of narrowly dispersed populations is determined and a multi-modal dispersion of five components is completely analyzed. A 360/sup 0/ light scattering photometer for analysis of single particles has been designed and developed. A fluid stream containing single particles intersects a focused laser beam at the primary focal point of an ellipsoidal reflector ring. The light scattered at angles theta = 2.5/sup 0/ to 177.5/sup 0/ at phi = 0/sup 0/ and 180/sup 0/ is reflected onto a circular array of photodiodes. The ellipsoidal reflector is situated in a chamber filled with fluid matching that of the stream to minimize refracting and reflecting interfaces. The detector array consists of 60 photodiodes each subtending 3/sup 0/ in scattering angle on 6/sup 0/ centers around 360/sup 0/. 32 measurements on individual particles can be acquired at rates of 500 particles per second. The intensity and angular distribution of light scattered by spherical particles are indicative of size and relative refractive index. Calculations, using Lorenz--Mie theory, of differential scattering patterns integrated over angle corresponding to the detector geometry determined the instrument response to particle size. From this the expected resolution and experimental procedures are determined.Ultimately, the photometer will be utilized for identification and discrimination of biological cells based on the sensitivity of light scattering to size, shape, refractive index differences, internal granularity, and other internal morphology. This study has demonstrated the utility of the photometer and indicates potential for application to light scattering studies of biological cells.

  5. Quantitative diagnosis of HER2 protein expressing breast cancer by single-particle quantum dot imaging.

    Science.gov (United States)

    Miyashita, Minoru; Gonda, Kohsuke; Tada, Hiroshi; Watanabe, Mika; Kitamura, Narufumi; Kamei, Takashi; Sasano, Hironobu; Ishida, Takanori; Ohuchi, Noriaki

    2016-10-01

    Overexpression of HER2 is one of the major causes of breast cancer, and therefore precise diagnosis of its protein expression level is important. However, current methods estimating the HER2-expression level are insufficient due to problem with the lack of quantification. This might result in a gap between diagnostics and therapeutics targeting HER2. Therefore, a new effective diagnostic method is needed. We developed a new immunohistochemical (IHC) technique with quantum dots (QD)-conjugated trastuzumab using single-particle imaging to quantitatively measure the HER2 expression level. Tissues from 37 breast cancer patients with available detailed clinical information were tested by IHC with QDs (IHC-QD) and the correlation with IHC with 3,3'-diaminobenzidine (DAB), fluorescence in situ hybridization (FISH), and IHC-QD was examined. The number of QD-conjugated trastuzumab particles binding specifically to a cancer cell was precisely calculated as the IHC-QD score. The IHC-QD score in 37 cases was correlated proportionally with the score of HER2 gene copy number as assessed by FISH (R = 0.83). When HER2 positivity was judged to be positive, the IHC-QD score with our cut-off level was exactly concordant with the FISH score with a cut-off value of 2.0. Furthermore, IHC-QDs score and time to progression (TTP) of trastuzumab therapy were well correlated in HER2-positive cases (R = 0.69). Conversely, the correlation between FISH score and TTP was not observed. We developed a precisely quantitative IHC method using trastuzumab-conjugated QDs and single-particle imaging analysis and propose the possibility of using IHC-QDs score as a predictive factor for trastuzumab therapy. © 2016 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

  6. Characteristics of tyre dust in polluted air: Studies by single particle mass spectrometry (ATOFMS)

    Science.gov (United States)

    Dall'Osto, Manuel; Beddows, David C. S.; Gietl, Johanna K.; Olatunbosun, Oluremi A.; Yang, Xiaoguang; Harrison, Roy M.

    2014-09-01

    There is a paucity of quantitative knowledge on the contributions of non-exhaust (abrasion and re-suspension) sources to traffic emissions. Abrasive emissions can be broadly categorised as tyre wear, brake wear and road dust/road surface wear. Current research often considers road dust and tyre dust as externally mixed particles, the former mainly composed of mineral matter and the latter solely composed of mainly organic matter and some trace elements. The aim of this work was to characterise tyre wear from both laboratory and field studies by using Aerosol Time-Of-Flight Mass Spectrometry (ATOFMS). Real-time single particle chemical composition was obtained from a set of rubber tyres rotating on a metal surface. Bimodal particle number size distributions peaking at 35 nm and 85 nm were obtained from SMPS/APS measurements over the range 6-20,000 nm. ATOFMS mass spectra of tyre wear in the particle size range 200-3000 nm diameter show peaks due to exo-sulphur compounds, nitrate, Zn and ions of high molecular weight (m/z > 100) attributed to organic polymers. Two large ATOFMS datasets collected from a number of outdoor studies were examined. The former was constituted of 48 road dust samples collected on the roads of London. The latter consisted of ATOFMS ambient air field studies from Europe, overall composed of more than 2,000,000 single particle mass spectra. The majority (95%) of tyre wear particles present in the road dust samples and atmospheric samples are internally mixed with metals (Li, Na, Ca, Fe, Ti), as well as phosphate. It is concluded that the interaction of tyres with the road surface creates particles internally mixed from two sources: tyre rubber and road surface materials. Measurements of the tyre rubber component alone may underestimate the contribution of tyre wear to concentrations of airborne particulate matter. The results presented are especially relevant for urban aerosol source apportionment and PM2.5 exposure assessment.

  7. Multiparameter Quantification of Liposomal Nanomedicines at the Single-Particle Level by High-Sensitivity Flow Cytometry.

    Science.gov (United States)

    Chen, Chaoxiang; Zhu, Shaobin; Wang, Shuo; Zhang, Wenqiang; Cheng, Yu; Yan, Xiaomei

    2017-04-26

    Drug-encapsulated liposomes have been considered the most clinically acceptable drug-delivery systems. However, current methods fall short in the quantitative characterization of individual nanoliposomes because of their small sizes and large heterogeneity. Here, we report a high-throughput method for the absolute quantification of particle size, drug content, fraction of drug encapsulation, and particle concentration of liposomal nanomedicines at the single-particle level. A laboratory-built high-sensitivity flow cytometer was used to simultaneously detect the side-scatter and fluorescence signals generated by individual nanomedicine particles at a speed up to 10 000 nanoparticles/min. To cope with the size dependence of the refractive index of liposomal nanomedicines, different sizes of doxorubicin-loaded liposomes were fabricated and characterized to serve as the calibration standards for the measurement of both particle size and drug content. This method provides a highly practical platform for the characterization of liposomal nanomedicines, and broad applications can be envisioned.

  8. Single particle ICP-MS characterization of titanium dioxide, silver, and gold nanoparticles during drinking water treatment.

    Science.gov (United States)

    Donovan, Ariel R; Adams, Craig D; Ma, Yinfa; Stephan, Chady; Eichholz, Todd; Shi, Honglan

    2016-02-01

    One of the most direct means for human exposure to nanoparticles (NPs) released into the environment is drinking water. Therefore, it is critical to understand the occurrence and fate of NPs in drinking water systems. The objectives of this study were to develop rapid and reliable analytical methods and apply them to investigate the fate and transportation of NPs during drinking water treatments. Rapid single particle ICP-MS (SP-ICP-MS) methods were developed to characterize and quantify titanium-containing, titanium dioxide, silver, and gold NP concentration, size, size distribution, and dissolved metal element concentration in surface water and treated drinking water. The effectiveness of conventional drinking water treatments (including lime softening, alum coagulation, filtration, and disinfection) to remove NPs from surface water was evaluated using six-gang stirrer jar test simulations. The selected NPs were nearly completely (97 ± 3%) removed after lime softening and alum coagulation/activated carbon adsorption treatments. Additionally, source and drinking waters from three large drinking water treatment facilities utilizing similar treatments with the simulation test were collected and analyzed by the SP-ICP-MS methods. Ti-containing particles and dissolved Ti were present in the river water samples, but Ag and Au were not present. Treatments used at each drinking water treatment facility effectively removed over 93% of the Ti-containing particles and dissolved Ti from the source water. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. 4 Gbps Impulse Radio (IR) Ultra-Wideband (UWB) Transmission over 100 Meters Multi Mode Fiber with 4 Meters Wireless Transmission

    DEFF Research Database (Denmark)

    Jensen, Jesper Bevensee; Rodes Lopez, Roberto; Caballero Jambrina, Antonio

    2009-01-01

    We present experimental demonstrations of in-building impulse radio (IR) ultra-wideband (UWB) link consisting of 100 m multi mode fiber (MMF) and 4 m wireless transmission at a record 4 Gbps, and a record 8 m wireless transmission at 2.5 Gbps. A directly modulated vertical cavity surface emitting...

  10. Evaluation of Aerosol Mixing State Classes in the GISS Modele-matrix Climate Model Using Single-particle Mass Spectrometry Measurements

    Science.gov (United States)

    Bauer, Susanne E.; Ault, Andrew; Prather, Kimberly A.

    2013-01-01

    Aerosol particles in the atmosphere are composed of multiple chemical species. The aerosol mixing state, which describes how chemical species are mixed at the single-particle level, provides critical information on microphysical characteristics that determine the interaction of aerosols with the climate system. The evaluation of mixing state has become the next challenge. This study uses aerosol time-of-flight mass spectrometry (ATOFMS) data and compares the results to those of the Goddard Institute for Space Studies modelE-MATRIX (Multiconfiguration Aerosol TRacker of mIXing state) model, a global climate model that includes a detailed aerosol microphysical scheme. We use data from field campaigns that examine a variety of air mass regimens (urban, rural, and maritime). At all locations, polluted areas in California (Riverside, La Jolla, and Long Beach), a remote location in the Sierra Nevada Mountains (Sugar Pine) and observations from Jeju (South Korea), the majority of aerosol species are internally mixed. Coarse aerosol particles, those above 1 micron, are typically aged, such as coated dust or reacted sea-salt particles. Particles below 1 micron contain large fractions of organic material, internally-mixed with sulfate and black carbon, and few external mixtures. We conclude that observations taken over multiple weeks characterize typical air mass types at a given location well; however, due to the instrumentation, we could not evaluate mass budgets. These results represent the first detailed comparison of single-particle mixing states in a global climate model with real-time single-particle mass spectrometry data, an important step in improving the representation of mixing state in global climate models.

  11. Single-particle properties of N = 12 to N = 20 silicon isotopes within the dispersive optical model

    Science.gov (United States)

    Bespalova, O. V.; Ermakova, T. A.; Klimochkina, A. A.; Spasskaya, T. I.

    2017-09-01

    Experimental neutron and proton single-particle energies in N = 12 to N = 20 silicon isotopes and data on neutron and proton scattering by nuclei of the isotope 28Si are analyzed on the basis of the dispersive optical model. Good agreement with available experimental data was attained. The occupation probabilities calculated for the single-particle states in question suggest a parallel-type filling of the 1 d and 2 s 1/2 neutron states in the isotopes 26,28,30,32,34Si. The single-particle spectra being considered are indicative of the closure of the Z = 14 proton subshell in the isotopes 30,32,34Si and the N = 20 neutron shell.

  12. Detection and characterisation of aluminium-containing nanoparticles in Chinese noodles by single particle ICP-MS

    DEFF Research Database (Denmark)

    Löschner, Katrin; Correia, Manuel; López Chaves, Carlos

    2018-01-01

    This study investigated Chinese noodles for the presence of aluminium-containing nanoparticles by using inductively coupled plasma mass spectrometry in single particle mode (spICP-MS) after enzymatic digestion by α-amylase. The aluminium concentrations in the noodle samples, determined by convent......This study investigated Chinese noodles for the presence of aluminium-containing nanoparticles by using inductively coupled plasma mass spectrometry in single particle mode (spICP-MS) after enzymatic digestion by α-amylase. The aluminium concentrations in the noodle samples, determined......-containing particles in food by spICP-MS....

  13. Body-insensitive Multi-Mode MIMO Terminal Antenna of Double-Ring Structure

    DEFF Research Database (Denmark)

    Zhao, Kun; Zhang, Shuai; Ishimiya, Katsunori

    2015-01-01

    In this paper, we propose a novel multimode multi-input multi-output (MIMO) antenna system composed of a dual-element MIMO cellular antenna and dual-element MIMO Wi-Fi antenna for mobile terminal applications. The antenna system has a double-ring structure and can be integrated with the metal frame...... of mobile terminals. With the multimode excitation, the MIMO cellular antenna can operate at 830-900 MHz, 1700-2200 MHz, and 2400-2700 MHz, for 2G, 3G, and LTE bands, respectively. The MIMO Wi-Fi antenna can cover two Wi-Fi bands from 2.4 to 2.5 GHz and from 5.2 to 5.8 GHz. The effect of a user's body...

  14. Technical Note: The single particle soot photometer fails to reliably detect PALAS soot nanoparticles

    Directory of Open Access Journals (Sweden)

    M. Gysel

    2012-12-01

    Full Text Available The single particle soot photometer (SP2 uses laser-induced incandescence (LII for the measurement of atmospheric black carbon (BC particles. The BC mass concentration is obtained by combining quantitative detection of BC mass in single particles with a counting efficiency of 100% above its lower detection limit. It is commonly accepted that a particle must contain at least several tenths of a femtogram BC in order to be detected by the SP2.

    Here we show the result that most BC particles from a PALAS spark discharge soot generator remain undetected by the SP2, even if their BC mass, as independently determined with an aerosol particle mass analyser (APM, is clearly above the typical lower detection limit of the SP2. Comparison of counting efficiency and effective density data of PALAS soot with flame generated soot (combustion aerosol standard burner, CAST, fullerene soot and carbon black particles (Cabot Regal 400R reveals that particle morphology can affect the SP2's lower detection limit. PALAS soot particles are fractal-like agglomerates of very small primary particles with a low fractal dimension, resulting in a very low effective density. Such loosely packed particles behave like "the sum of individual primary particles" in the SP2's laser. Accordingly, most PALAS soot particles remain undetected as the SP2's laser intensity is insufficient to heat the primary particles to their vaporisation temperature because of their small size (Dpp ≈ 5–10 nm. Previous knowledge from pulsed laser-induced incandescence indicated that particle morphology might have an effect on the SP2's lower detection limit, however, an increase of the lower detection limit by a factor of ∼5–10, as reported here for PALAS soot, was not expected.

    In conclusion, the SP2's lower detection limit at a certain laser power depends primarily on the total BC mass per particle for compact particles with sufficiently high effective

  15. Radiation damage in single-particle cryo-electron microscopy: effects of dose and dose rate

    International Nuclear Information System (INIS)

    Karuppasamy, Manikandan; Karimi Nejadasl, Fatemeh; Vulovic, Milos; Koster, Abraham J.; Ravelli, Raimond B. G.

    2011-01-01

    The effects of dose and dose-rate were investigated for single-particle cryo-electron microscopy using stroboscopic data collection. A dose-rate effect was observed favoring lower flux densities. Radiation damage is an important resolution limiting factor both in macromolecular X-ray crystallography and cryo-electron microscopy. Systematic studies in macromolecular X-ray crystallography greatly benefited from the use of dose, expressed as energy deposited per mass unit, which is derived from parameters including incident flux, beam energy, beam size, sample composition and sample size. In here, the use of dose is reintroduced for electron microscopy, accounting for the electron energy, incident flux and measured sample thickness and composition. Knowledge of the amount of energy deposited allowed us to compare doses with experimental limits in macromolecular X-ray crystallography, to obtain an upper estimate of radical concentrations that build up in the vitreous sample, and to translate heat-transfer simulations carried out for macromolecular X-ray crystallography to cryo-electron microscopy. Stroboscopic exposure series of 50–250 images were collected for different incident flux densities and integration times from Lumbricus terrestris extracellular hemoglobin. The images within each series were computationally aligned and analyzed with similarity metrics such as Fourier ring correlation, Fourier ring phase residual and figure of merit. Prior to gas bubble formation, the images become linearly brighter with dose, at a rate of approximately 0.1% per 10 MGy. The gradual decomposition of a vitrified hemoglobin sample could be visualized at a series of doses up to 5500 MGy, by which dose the sample was sublimed. Comparison of equal-dose series collected with different incident flux densities showed a dose-rate effect favoring lower flux densities. Heat simulations predict that sample heating will only become an issue for very large dose rates (50 e − Å −2 s

  16. Single-particle characterization of the high-Arctic summertime aerosol

    Directory of Open Access Journals (Sweden)

    B. Sierau

    2014-07-01

    Full Text Available Single-particle mass-spectrometric measurements were carried out in the high Arctic north of 80° during summer 2008. The campaign took place onboard the icebreaker Oden and was part of the Arctic Summer Cloud Ocean Study (ASCOS. The instrument deployed was an aerosol time-of-flight mass spectrometer (ATOFMS that provides information on the chemical composition of individual particles and their mixing state in real time. Aerosols were sampled in the marine boundary layer at stations in the open ocean, in the marginal ice zone, and in the pack ice region. The largest fraction of particles detected for subsequent analysis in the size range of the ATOFMS between approximately 200 and 3000 nm in diameter showed mass-spectrometric patterns, indicating an internal mixing state and a biomass burning and/or biofuel source. The majority of these particles were connected to an air mass layer of elevated particle concentration mixed into the surface mixed layer from the upper part of the marine boundary layer. The second largest fraction was represented by sea salt particles. The chemical analysis of the over-ice sea salt aerosol revealed tracer compounds that reflect chemical aging of the particles during their long-range advection from the marginal ice zone, or open waters south thereof prior to detection at the ship. From our findings we conclude that long-range transport of particles is one source of aerosols in the high Arctic. To assess the importance of long-range particle sources for aerosol–cloud interactions over the inner Arctic in comparison to local and regional biogenic primary aerosol sources, the chemical composition of the detected particles was analyzed for indicators of marine biological origin. Only a minor fraction showed chemical signatures of potentially ocean-derived primary particles of that kind. However, a chemical bias in the ATOFMS's detection capabilities observed during ASCOS might suggest the presence of a particle type of

  17. Measurement of ambient aerosols in northern Mexico City by single particle mass spectrometry

    Directory of Open Access Journals (Sweden)

    R. C. Moffet

    2008-08-01

    Full Text Available Continuous ambient measurements with aerosol time-of-flight mass spectrometry (ATOFMS were made in an industrial/residential section in the northern part of Mexico City as part of the Mexico City Metropolitan Area-2006 campaign (MCMA-2006. Results are presented for the period of 15–27 March 2006. The submicron size mode contained both fresh and aged biomass burning, aged organic carbon (OC mixed with nitrate and sulfate, elemental carbon (EC, nitrogen-organic carbon, industrial metal, and inorganic NaK inorganic particles. Overall, biomass burning and aged OC particle types comprised 40% and 31%, respectively, of the submicron mode. In contrast, the supermicron mode was dominated by inorganic NaK particle types (42% which represented a mixture of dry lake bed dust and industrial NaK emissions mixed with soot. Additionally, aluminosilicate dust, transition metals, OC, and biomass burning contributed to the supermicron particles. Early morning periods (2–6 a.m. showed high fractions of inorganic particles from industrial sources in the northeast, composed of internal mixtures of Pb, Zn, EC and Cl, representing up to 73% of the particles in the 0.2–3μm size range. A unique nitrogen-containing organic carbon (NOC particle type, peaking in the early morning hours, was hypothesized to be amines from local industrial emissions based on the time series profile and back trajectory analysis. A strong dependence on wind speed and direction was observed in the single particle types that were present during different times of the day. The early morning (3:30–10 a.m. showed the greatest contributions from industrial emissions. During mid to late mornings (7–11 a.m., weak northerly winds were observed along with the most highly aged particles. Stronger winds from the south picked up in the late morning (after 11 a.m., resulting in a decrease in the concentrations of the major aged particle types and an increase in the number fraction of fresh

  18. Mission Capability Gains from Multi-Mode Propulsion Thrust Variations on a Variety Spacecraft Orbital Maneuvers

    Science.gov (United States)

    2011-03-01

    a 30 cm derated ion thruster, a xenon Hall thruster (SPT-100), and a hydrazine arcjet thruster. Repositioning a spacecraft in a sun synchronous low ...4000, which has a high thrust/high power mode and a low thrust/ low power mode. The maximum thrust on this system was around 0.3 N and has had...Small Satellite LEO Maneuvers with Low - Power Electric Propulsion,” 44th AIAA/ASME/SAE/ASEE Joint Propulsion Conference, AIAA 2008-4516.     99

  19. Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking.

    Science.gov (United States)

    von Diezmann, Alex; Shechtman, Yoav; Moerner, W E

    2017-06-14

    Single-molecule super-resolution fluorescence microscopy and single-particle tracking are two imaging modalities that illuminate the properties of cells and materials on spatial scales down to tens of nanometers or with dynamical information about nanoscale particle motion in the millisecond range, respectively. These methods generally use wide-field microscopes and two-dimensional camera detectors to localize molecules to much higher precision than the diffraction limit. Given the limited total photons available from each single-molecule label, both modalities require careful mathematical analysis and image processing. Much more information can be obtained about the system under study by extending to three-dimensional (3D) single-molecule localization: without this capability, visualization of structures or motions extending in the axial direction can easily be missed or confused, compromising scientific understanding. A variety of methods for obtaining both 3D super-resolution images and 3D tracking information have been devised, each with their own strengths and weaknesses. These include imaging of multiple focal planes, point-spread-function engineering, and interferometric detection. These methods may be compared based on their ability to provide accurate and precise position information on single-molecule emitters with limited photons. To successfully apply and further develop these methods, it is essential to consider many practical concerns, including the effects of optical aberrations, field dependence in the imaging system, fluorophore labeling density, and registration between different color channels. Selected examples of 3D super-resolution imaging and tracking are described for illustration from a variety of biological contexts and with a variety of methods, demonstrating the power of 3D localization for understanding complex systems.

  20. Multi-Mode Electric Actuator Dynamic Modelling for Missile Fin Control

    Directory of Open Access Journals (Sweden)

    Bhimashankar Gurav

    2017-06-01

    Full Text Available Linear first/second order fin direct current (DC actuator model approximations for missile applications are currently limited to angular position and angular velocity state variables. Furthermore, existing literature with detailed DC motor models is decoupled from the application of interest: tail controller missile lateral acceleration (LATAX performance. This paper aims to integrate a generic DC fin actuator model with dual-mode feedforward and feedback control for tail-controlled missiles in conjunction with the autopilot system design. Moreover, the characteristics of the actuator torque information in relation to the aerodynamic fin loading for given missile trim velocities are also provided. The novelty of this paper is the integration of the missile LATAX autopilot states and actuator states including the motor torque, position and angular velocity. The advantage of such an approach is the parametric analysis and suitability of the fin actuator in relation to the missile lateral acceleration dynamic behaviour.

  1. Catastrophic optical bulk degradation in high-power single- and multi-mode InGaAs-AlGaAs strained QW lasers: part II

    Science.gov (United States)

    Sin, Yongkun; Ayvazian, Talin; Brodie, Miles; Lingley, Zachary

    2018-03-01

    High-power single-mode (SM) and multi-mode (MM) InGaAs-AlGaAs strained quantum well (QW) lasers are critical components for both terrestrial and space satellite communications systems. Since these lasers predominantly fail by catastrophic and sudden degradation due to catastrophic optical damage (COD), it is especially crucial for space satellite applications to investigate reliability, failure modes, precursor signatures of failure, and degradation mechanisms of these lasers. Our group reported a new failure mode in MM and SM InGaAs-AlGaAs strained QW lasers in 2009 and 2016, respectively. Our group also reported in 2017 that bulk failure due to catastrophic optical bulk damage (COBD) is the dominant failure mode of both SM and MM lasers that were subject to long-term life-tests. For the present study, we continued our physics of failure investigation by performing long-term life-tests followed by failure mode analysis (FMA) using nondestructive and destructive micro-analytical techniques. We performed long-term accelerated life-tests on state-of-the-art SM and MM InGaAs- AlGaAs strained QW lasers under ACC mode. Our life-tests have accumulated over 25,000 test hours for SM lasers and over 35,000 test hours for MM lasers. We first employed electron beam induced current (EBIC) technique to identify failure modes of degraded SM lasers by observing dark line defects. All the SM failures that we studied showed catastrophic and sudden degradation and all of these failures were bulk failures. Since degradation mechanisms responsible for COBD are still not well understood, we also employed other techniques including focused ion beam (FIB) and high-resolution TEM to further study dark line defects and dislocations in post-aged lasers. Keywor

  2. Characterization of biomass combustion at high temperatures based on an upgraded single particle model

    International Nuclear Information System (INIS)

    Li, Jun; Paul, Manosh C.; Younger, Paul L.; Watson, Ian; Hossain, Mamdud; Welch, Stephen

    2015-01-01

    Highlights: • High temperature rapid biomass combustion is studied based on single particle model. • Particle size changes in devolatilization and char oxidation models are addressed. • Time scales of various thermal sub-processes are compared and discussed. • Potential solutions are suggested to achieve better biomass co-firing performances. - Abstract: Biomass co-firing is becoming a promising solution to reduce CO 2 emissions, due to its renewability and carbon neutrality. Biomass normally has high moisture and volatile contents, complicating its combustion behavior, which is significantly different from that of coal. A computational fluid dynamics (CFD) combustion model of a single biomass particle is employed to study high-temperature rapid biomass combustion. The two-competing-rate model and kinetics/diffusion model are used to model biomass devolatilization reaction and char burnout process, respectively, in which the apparent kinetics used for those two models were from high temperatures and high heating rates tests. The particle size changes during the devolatilization and char burnout are also considered. The mass loss properties and temperature profile during the biomass devolatilization and combustion processes are predicted; and the timescales of particle heating up, drying, devolatilization, and char burnout are compared and discussed. Finally, the results shed light on the effects of particle size on the combustion behavior of biomass particle

  3. Real-time analysis of insoluble particles in glacial ice using single-particle mass spectrometry

    Science.gov (United States)

    Osman, Matthew; Zawadowicz, Maria A.; Das, Sarah B.; Cziczo, Daniel J.

    2017-11-01

    Insoluble aerosol particles trapped in glacial ice provide insight into past climates, but analysis requires information on climatically relevant particle properties, such as size, abundance, and internal mixing. We present a new analytical method using a time-of-flight single-particle mass spectrometer (SPMS) to determine the composition and size of insoluble particles in glacial ice over an aerodynamic size range of ˜ 0.2-3.0 µm diameter. Using samples from two Greenland ice cores, we developed a procedure to nebulize insoluble particles suspended in melted ice, evaporate condensed liquid from those particles, and transport them to the SPMS for analysis. We further determined size-dependent extraction and instrument transmission efficiencies to investigate the feasibility of determining particle-class-specific mass concentrations. We find SPMS can be used to provide constraints on the aerodynamic size, composition, and relative abundance of most insoluble particulate classes in ice core samples. We describe the importance of post-aqueous processing to particles, a process which occurs due to nebulization of aerosols from an aqueous suspension of originally soluble and insoluble aerosol components. This study represents an initial attempt to use SPMS as an emerging technique for the study of insoluble particulates in ice cores.

  4. Single Particle Laser Mass Spectrometry Applied to Differential Ice Nucleation Experiments at the AIDA Chamber

    International Nuclear Information System (INIS)

    Gallavardin, S. J.; Froyd, Karl D.; Lohmann, U.; Moehler, Ottmar; Murphy, Daniel M.; Cziczo, Dan

    2008-01-01

    Experiments conducted at the Aerosol Interactions and Dynamics in the Atmosphere (AIDA) chamber located in Karlsruhe, Germany permit investigation of particle properties that affect the nucleation of ice at temperature and water vapor conditions relevant to cloud microphysics and climate issues. Ice clouds were generated by heterogeneous nucleation of Arizona test dust (ATD), illite, and hematite and homogeneous nucleation of sulfuric acid. Ice crystals formed in the chamber were inertially separated from unactivated, or 'interstitial' aerosol particles with a pumped counterflow virtual impactor (PCVI), then evaporated. The ice residue (i.e., the aerosol which initiated ice nucleation plus any material which was scavenged from the gas- and/or particle-phase), was chemically characterized at the single particle level using a laser ionization mass spectrometer. In this manner the species that first nucleated ice could be identified out of a mixed aerosol population in the chamber. Bare mineral dust particles were more effective ice nuclei (IN) than similar particles with a coating. Metallic particles from contamination in the chamber initiated ice nucleation before other species but there were few enough that they did not compromise the experiments. Nitrate, sulfate, and organics were often detected on particles and ice residue, evidently from scavenging of trace gas-phase species in the chamber. Hematite was a more effective ice nucleus than illite. Ice residue was frequently larger than unactivated test aerosol due to the formation of aggregates due to scavenging, condensation of contaminant gases, and the predominance of larger aerosol in nucleation

  5. Analysis and differentiation of mineral dust by single particle laser mass spectrometry

    International Nuclear Information System (INIS)

    Gallavardin, S. J.; Lohmann, U.; Cziczo, Daniel J.

    2008-01-01

    This study evaluates the potential of single particle laser desorption/ionization mass spectrometry for the analysis of atmospherically relevant mineral dusts. Samples of hematite, goethite, calcium carbonate, calcium sulfate, silica, quartz, montmorrillonite, kaolinite, illite, hectorite, wollastonite and nephelinsyenit were investigated in positive and negative ion mode with a monopolar time-of-flight mass spectrometer where the desorption/ionization step was performed with a 193 nm excimer laser (∼10 9 W/cm 2 ). Particle size ranged from 500 nm to 3 (micro)m. Positive mass spectra mainly provide elemental composition whereas negative ion spectra provide information on element speciation and of a structural nature. The iron oxide, calcium-rich and aluminosilicate nature of particles is established in positive ion mode. The differentiation of calcium materials strongly relies on the calcium counter-ions in negative mass spectra. Aluminosilicates can be differentiated in both positive and negative ion mode using the relative abundance of various aluminum and silicon ions

  6. Investigating single-particle structure in 26Na using the new SHARC array

    International Nuclear Information System (INIS)

    Wilson, G.L.; Catford, W.N.; Diget, C.Aa.

    2015-01-01

    The changing of the nuclear shells for light, neutron-rich nuclei, and the single-particle nature of 26 Na, has been explored by studying 25 Na(d, p) 26 Na in inverse kinematics, using a beam of 25 Na ions at 5 MeV per nucleon, provided by the ISAC-II facility at TRIUMF, Vancouver. Charged particles were detected with a highly-segmented silicon array that surrounded the 0.5 mg/cm 2 (CD 2 ) n target. Gamma rays from the recoiling 26 Na nucleus were detected using eight Compton-suppressed HPGe clover detectors. Recoil tagging was provided by an in-beam scintillation foil, downstream of the germanium array. A novel technique of utilising pγ- and pγγ-gating to extract proton angular distributions from states populated close in energy was employed with success. New states in 26 Na that are populated directly have been identified, using γ-decay patterns. Shell model calculations for comparison to experimental results are ongoing, using different model bases. (author)

  7. Investigating Single-Particle Structure in 26Na Using the New SHARC Array

    Science.gov (United States)

    Wilson, G. L.; Catford, W. N.; Diget, C. Aa.; Orr, N. A.; Matta, A.; Hackman, G.; Williams, S. J.; Simpson, E. C.; Celik, I. C.; Achouri, N. L.; Adsley, P.; Al-Falou, H.; Ashley, R.; Austin, R. A. E.; Ball, G. C.; Blackmon, J. C.; Boston, A. J.; Boston, H. C.; Brown, S. M.; Cross, D. S.; Djongolov, M.; Drake, T. E.; Hager, U.; Fox, S. P.; Fulton, B. R.; Galinski, N.; Garnsworthy, A. B.; Jamieson, D.; Kanungo, R.; Leach, K.; Orce, J. N.; Pearson, C. J.; Porter-Peden, M.; Sarazin, F.; Sjue, S.; Smalley, D.; Sumithrarachchi, C.; Triambak, S.; Unsworth, C.; Wadsworth, R.

    The changing of the nuclear shells for light, neutron-rich nuclei, and the single-particle nature of 26Na, has been explored by studying 25Na(d, p)26Na in inverse kinematics, using a beam of 25Na ions at 5 MeV per nucleon, provided by the ISAC-II facility at TRIUMF, Vancouver. Charged particles were detected with a highly-segmented silicon array that surrounded the 0.5 mg/cm2 (CD2)n target. Gamma rays from the recoiling 26Na nucleus were detected using eight Compton-suppressed HPGe clover detectors. Recoil tagging was provided by an in-beam scintillation foil, downstream of the germanium array. A novel technique of utilising pγ- and pγγ-gating to extract proton angular distributions from states populated close in energy was employed with success. New states in 26Na that are populated directly have been identified, using γ-decay patterns. Shell model calculations for comparison to experimental results are ongoing, using different model bases.

  8. Single Particle Transport Through Carbon Nanotube Wires: Effect of Defects and Polyhedral Cap

    Science.gov (United States)

    Anantram, M. P.; Govidan, T. R.

    1999-01-01

    The ability to manipulate carbon nanotubes with increasing precision has enabled a large number of successful electron transport experiments. These studies have primarily focussed on characterizing transport through both metallic and semiconducting wires. Tans et al. demonstrated ballistic transport in single-wall nanotubes for the first time, although the experimental configuration incurred large contact resistance. Subsequently, methods of producing low contact resistances have been developed and two terminal conductances smaller than 50 k-ohms have been repeatably demonstrated in single-wall and multi-wall nanotubes. In multi-wall nanotubes, Frank et al. demonstrated a resistance of approximately h/2e(exp 2) in a configuration where the outermost layer made contact to a liquid metal. This was followed by the work of de Pablo et al. where a resistance of h(bar)/27e(exp 2) (approximately 478 ohms) was measured in a configuration where electrical contact was made to many layers of a multi-wall nanotube. Frank et al. and Pablo et al. note that each conducting layer contributes a conductance of only 2e(exp 2)/h, instead of the 4e(exp 2)/h that a single particle mode counting picture yields. These small resistances have been obtained in microns long nanotubes, making them the best conducting molecular wires to date. The large conductance of nanotube wires stems from the fact that the crossing bands of nanotubes are robust to defect scattering.

  9. Particle interactions of fluticasone propionate and salmeterol xinafoate detected with single particle aerosol mass spectrometry (SPAMS).

    Science.gov (United States)

    Jetzer, Martin W; Morrical, Bradley D; Fergenson, David P; Imanidis, Georgios

    2017-10-30

    Particle co-associations between the active pharmaceutical ingredients fluticasone propionate and salmeterol xinafoate were examined in dry powder inhaled (DPI) and metered dose inhaled (MDI) combination products. Single Particle Aerosol Mass Spectrometry was used to investigate the particle interactions in Advair Diskus ® (500/50 mcg) and Seretide ® (125/25 mcg). A simple rules tree was used to identify each compound, either alone or co-associated at the level of the individual particle, using unique marker peaks in the mass spectra for the identification of each drug. High levels of drug particle co-association (fluticasone-salmeterol) were observed in the aerosols emitted from Advair Diskus ® and Seretide ® . The majority of the detected salmeterol particles were found to be in co-association with fluticasone in both tested devices. Another significant finding was that rather coarse fluticasone particles (in DPI) and fine salmeterol particles (both MDI and DPI) were forming the particle co-associations. Copyright © 2017 Elsevier B.V. All rights reserved.

  10. Robust model-based analysis of single-particle tracking experiments with Spot-On.

    Science.gov (United States)

    Hansen, Anders S; Woringer, Maxime; Grimm, Jonathan B; Lavis, Luke D; Tjian, Robert; Darzacq, Xavier

    2018-01-04

    Single-particle tracking (SPT) has become an important method to bridge biochemistry and cell biology since it allows direct observation of protein binding and diffusion dynamics in live cells. However, accurately inferring information from SPT studies is challenging due to biases in both data analysis and experimental design. To address analysis bias, we introduce 'Spot-On', an intuitive web-interface. Spot-On implements a kinetic modeling framework that accounts for known biases, including molecules moving out-of-focus, and robustly infers diffusion constants and subpopulations from pooled single-molecule trajectories. To minimize inherent experimental biases, we implement and validate stroboscopic photo-activation SPT (spaSPT), which minimizes motion-blur bias and tracking errors. We validate Spot-On using experimentally realistic simulations and show that Spot-On outperforms other methods. We then apply Spot-On to spaSPT data from live mammalian cells spanning a wide range of nuclear dynamics and demonstrate that Spot-On consistently and robustly infers subpopulation fractions and diffusion constants. © 2018, Hansen et al.

  11. Single particle transfer reactions: what can they tell us about vibrational states

    International Nuclear Information System (INIS)

    Hering, W.R.

    1975-01-01

    The topic discussed concerns single particle transfer reactions (SPTR) which are, in general, used to study SP states. However, good SP states are rare objects in nature and people who try to look for them have often to settle with something less than ideal. Indeed the picture of a pure SP state is physically not even reasonable. It means that a nucleon is moving around a core nucleus which stays in its ground state: a process which one could call equivalent to elastic scattering of a nucleon which is not free but rather in a bound state. However it is shown that inelastic scattering is a very strong competitor to elastic scattering if the nucleus possesses states of high collectivity. Thus one would expect inelastic scattering to happen also while the nucleon is bound. This is a very intuitive picture of what is called the fragmentation of SP states. A final state psi sub(B) is populated by the transfer reaction A + a → B + b where psi sub(B) = α 1 phi 1 phi sub(A)(0) + α 2 phi 2 phi sub(A)(lambda). Hence the population of psi sub(B) automatically involves the collective state phi sub(A)(lambda). A discussion of how one can get information about phi sub(A)(lambda) out of the experimental data is given. (Auth.)

  12. Refined source apportionment of coal combustion sources by using single particle mass spectrometry.

    Science.gov (United States)

    Xu, Jiao; Wang, Haiting; Li, Xiujian; Li, Yue; Wen, Jie; Zhang, Jinsheng; Shi, Xurong; Li, Mei; Wang, Wei; Shi, Guoliang; Feng, Yinchang

    2018-06-15

    In this study, samples of three typical coal combustion source types, including Domestic bulk coal combustion (DBCC), Heat supply station (HSS), and Power plant (PP) were sampled and large sets of their mass spectra were obtained and analyzed by SPAMS during winter in a megacity in China. A primary goal of this study involves determining representative size-resolved single particle mass spectral signatures of three source types that can be used in source apportionment activities. Chemical types describe the majority of the particles of each source type were extracted by ART-2a algorithm with distinct size characteristics, and the corresponding tracer signals were identified. Mass spectral signatures from three source types were different from each other, and the tracer signals were effective in distinguishing different source types. A high size-resolution source apportionment method were proposed in this study through matching sources' mass spectral signatures to particle spectra in a twelve days ambient sampling to source apportion the particles. Contributions of three source types got different size characteristics, as HSS source got higher contribution in smaller sizes, But PP source got higher contributions as size increased. Source contributions were also quantified during two typical haze episodes, and results indicated that HSS source (for central-heating) and DBCC source (for domestic heating and cooking) may contribute evidently to pollution formation. Copyright © 2018. Published by Elsevier B.V.

  13. Deblurring of class-averaged images in single-particle electron microscopy

    International Nuclear Information System (INIS)

    Park, Wooram; Chirikjian, Gregory S; Madden, Dean R; Rockmore, Daniel N

    2010-01-01

    This paper proposes a method for the deblurring of class-averaged images in single-particle electron microscopy (EM). Since EM images of biological samples are very noisy, the images which are nominally identical projection images are often grouped, aligned and averaged in order to cancel or reduce the background noise. However, the noise in the individual EM images generates errors in the alignment process, which creates an inherent limit on the accuracy of the resulting class averages. This inaccurate class average due to the alignment errors can be viewed as the result of a convolution of an underlying clear image with a blurring function. In this work, we develop a deconvolution method that gives an estimate for the underlying clear image from a blurred class-averaged image using precomputed statistics of misalignment. Since this convolution is over the group of rigid-body motions of the plane, SE(2), we use the Fourier transform for SE(2) in order to convert the convolution into a matrix multiplication in the corresponding Fourier space. For practical implementation we use a Hermite-function-based image modeling technique, because Hermite expansions enable lossless Cartesian-polar coordinate conversion using the Laguerre–Fourier expansions, and Hermite expansion and Laguerre–Fourier expansion retain their structures under the Fourier transform. Based on these mathematical properties, we can obtain the deconvolution of the blurred class average using simple matrix multiplication. Tests of the proposed deconvolution method using synthetic and experimental EM images confirm the performance of our method

  14. Component tree analysis of cystovirus φ6 nucleocapsid Cryo-EM single particle reconstructions.

    Directory of Open Access Journals (Sweden)

    Lucas M Oliveira

    Full Text Available The 3-dimensional structure of the nucleocapsid (NC of bacteriophage φ6 is described utilizing component tree analysis, a topological and geometric image descriptor. The component trees are derived from density maps of cryo-electron microscopy single particle reconstructions. Analysis determines position and occupancy of structure elements responsible for RNA packaging and transcription. Occupancy of the hexameric nucleotide triphosphorylase (P4 and RNA polymerase (P2 are found to be essentially complete in the NC. The P8 protein lattice likely fixes P4 and P2 in place during maturation. We propose that the viral procapsid (PC is a dynamic structural intermediate where the P4 and P2 can attach and detach until held in place in mature NCs. During packaging, the PC expands to accommodate the RNA, and P2 translates from its original site near the inner 3-fold axis (20 sites to the inner 5-fold axis (12 sites with excess P2 positioned inside the central region of the NC.

  15. Single-Particle Tracking of Human Immunodeficiency Virus Type 1 Productive Entry into Human Primary Macrophages.

    Science.gov (United States)

    Li, Qin; Li, Wei; Yin, Wen; Guo, Jia; Zhang, Zhi-Ping; Zeng, Dejun; Zhang, Xiaowei; Wu, Yuntao; Zhang, Xian-En; Cui, Zongqiang

    2017-04-25

    Macrophages are one of the major targets of human immunodeficiency virus (HIV-1), but the viral entry pathway remains poorly understood in these cells. Noninvasive virus labeling and single-virus tracking are effective tools for studying virus entry. Here, we constructed a quantum dot (QD)-encapsulated infectious HIV-1 particle to track viral entry at a single-particle level in live human primary macrophages. QDs were encapsulated in HIV-1 virions by incorporating viral accessory protein Vpr-conjugated QDs during virus assembly. With the HIV-1 particles encapsulating QDs, we monitored the early phase of viral infection in real time and observed that, during infection, HIV-1 was endocytosed in a clathrin-mediated manner; the particles were translocated into Rab5A-positive endosomes, and the core was released into the cytoplasm by viral envelope-mediated endosomal fusion. Drug inhibition assays verified that endosome fusion contributes to HIV-1 productive infection in primary macrophages. Additionally, we observed that a dynamic actin cytoskeleton is critical for HIV-1 entry and intracellular migration in primary macrophages. HIV-1 dynamics and infection could be blocked by multiple different actin inhibitors. Our study revealed a productive entry pathway in macrophages that requires both endosomal function and actin dynamics, which may assist in the development of inhibitors to block the HIV entry in macrophages.

  16. Detection of Engineered Copper Nanoparticles in Soil Using Single Particle ICP-MS

    Directory of Open Access Journals (Sweden)

    Jana Navratilova

    2015-12-01

    Full Text Available Regulatory efforts rely on nanometrology for the development and implementation of laws regarding the incorporation of engineered nanomaterials (ENMs into industrial and consumer products. Copper is currently one of the most common metals used in the constantly developing and expanding sector of nanotechnology. The use of copper nanoparticles in products, such as agricultural biocides, cosmetics and paints, is increasing. Copper based ENMs will eventually be released to the environment through the use and disposal of nano-enabled products, however, the detection of copper ENMs in environmental samples is a challenging task. Single particle inductively coupled plasma mass spectroscopy (spICP-MS has been suggested as a powerful tool for routine nanometrology efforts. In this work, we apply a spICP-MS method for the detection of engineered copper nanomaterials in colloidal extracts from natural soil samples. Overall, copper nanoparticles were successfully detected in the soil colloidal extracts and the importance of dwell time, background removal, and sample dilution for method optimization and recovery maximization is highlighted.

  17. Development and applications of single particle orientation and rotational tracking in dynamic systems

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Kuangcai [Iowa State Univ., Ames, IA (United States)

    2016-02-19

    The goal of this study is to help with future data analysis and experiment designs in rotational dynamics research using DIC-based SPORT technique. Most of the current studies using DIC-based SPORT techniques are technical demonstrations. Understanding the mechanisms behind the observed rotational behaviors of the imaging probes should be the focus of the future SPORT studies. More efforts are still needed in the development of new imaging probes, particle tracking methods, instrumentations, and advanced data analysis methods to further extend the potential of DIC-based SPORT technique.

  18. Detection of lead nanoparticles in game meat by single particle ICP-MS following use of lead-containing bullets

    DEFF Research Database (Denmark)

    Kollander, Barbro; Widemo, Fredrik; Ågren, Erik

    2017-01-01

    This study investigated whether game meat may contain nanoparticles of lead from ammunition. Lead nanoparticles in the range 40 to 750 nm were detected by ICP-MS in single particle mode in game shot with lead-containing bullets. The median diameter of the detected nanoparticles was around 60 nm. ...

  19. Single Particle and PET-based Platform for Identifying Optimal Plasmonic Nano-Heaters for Photothermal Cancer Therapy

    DEFF Research Database (Denmark)

    Jørgensen, Jesper Tranekjær; Nørregaard, Kamilla; Tian, Pengfei

    2016-01-01

    Plasmonic nanoparticle-based photothermal cancer therapy is a promising new tool to inflict localized and irreversible damage to tumor tissue by hyperthermia, without harming surrounding healthy tissue. We developed a single particle and positron emission tomography (PET)-based platform to quanti...

  20. Inert gases in a terra sample - Measurements in six grain-size fractions and two single particles from Lunar 20.

    Science.gov (United States)

    Heymann, D.; Lakatos, S.; Walton, J. R.

    1973-01-01

    Review of the results of inert gas measurements performed on six grain-size fractions and two single particles from four samples of Luna 20 material. Presented and discussed data include the inert gas contents, element and isotope systematics, radiation ages, and Ar-36/Ar-40 systematics.

  1. Large-Scale Single Particle and Cell Trapping based on Rotating Electric Field Induced-Charge Electroosmosis.

    Science.gov (United States)

    Wu, Yupan; Ren, Yukun; Tao, Ye; Hou, Likai; Jiang, Hongyuan

    2016-12-06

    We propose a simple, inexpensive microfluidic chip for large-scale trapping of single particles and cells based on induced-charge electroosmosis in a rotating electric field (ROT-ICEO). A central floating electrode array, was placed in the center of the gap between four driving electrodes with a quadrature configuration and used to immobilize single particles or cells. Cells were trapped on the electrode array by the interaction between ROT-ICEO flow and buoyancy flow. We experimentally optimized the efficiency of trapping single particles by investigating important parameters like particle or cell density and electric potential. Experimental and numerical results showed good agreement. The operation of the chip was verified by trapping single polystyrene (PS) microspheres with diameters of 5 and 20 μm and single yeast cells. The highest single particle occupancy of 73% was obtained using a floating electrode array with a diameter of 20 μm with an amplitude voltage of 5 V and frequency of 10 kHz for PS microbeads with a 5-μm diameter and density of 800 particles/μL. The ROT-ICEO flow could hold cells against fluid flows with a rate of less than 0.45 μL/min. This novel, simple, robust method to trap single cells has enormous potential in genetic and metabolic engineering.

  2. Single particle deformation and analysis of the same silica coated gold nanorods before and after fs-laser pulse excitation

    NARCIS (Netherlands)

    Albrecht, W.; Deng, Tian-Song; Goris, Bart; van Huis, M.A.; Bals, Sarah; van Blaaderen, Alfons

    2016-01-01

    We performed single particle deformation experiments on silicacoated gold nanorods under femtosecond (fs) illumination. Changes in the particle shape were analyzed by electron microscopy and associated changes in the plasmon resonance by electron energy loss spectroscopy. Silica-coated rods were

  3. Development of a Charged Particle Microbeam for Single-Particle Subcellular Irradiations at the MIT Laboratory for Accelerator Beam Application

    International Nuclear Information System (INIS)

    Yanch, Jacquelyn C.

    2004-01-01

    The development of a charged particle microbeam for single particle, subcellular irradiations at the Massachusetts Institute of Technology Laboratory for Accelerator Beam Applications (MIT LABA) was initiated under this NEER aeard. The Microbeam apparatus makes use of a pre-existing electrostatic accelerator with a horizontal beam tube

  4. Optimization of plasma sampling depth and aerosol gas flow rates for single particle inductively coupled plasma mass spectrometry analysis.

    Science.gov (United States)

    Kálomista, Ildikó; Kéri, Albert; Galbács, Gábor

    2017-09-01

    We performed experiments to assess the separate and also the combined effect of the sampling depth and the aerosol gas flow rates on the signal formation in single particle inductively coupled plasma mass spectrometry (spICP-MS) measurements by using dispersions containing Ag and Au NPs. It was found that the NP signal can significantly be improved by the optimization of the sampling depth. With respect to the "robust" setting, a signal improvement of nearly 100% could be achieved, which translates into a 25-30% improvement in size detection limits. It was also found that the shape of the spICP-MS signal histograms also change with the change of the plasma sampling depth. It was demonstrated that nanoparticle peak separation can also be significantly enhanced by using sampling depth optimization. The effect of the aerosol dilution gas flow, now standard in most ICP-MS instruments, on the spICP-MS signal formation was also studied for the first time in the literature, as this flow was hoped to make spICP-MS measurements more practical and faster via the on-line dilution of the aerosol generated from nano-dispersions. Our experimental results revealed that the dilution gas flow can only be used for a moderate aerosol dilution in spICP-MS measurements, if the gas flow going to the pneumatic nebulizer is proportionally lowered at the same time. This however was found to cause a significant worsening in the operation of the sample introduction system, which gives rise to a strong NP signal loss. Thus it was concluded that the use of the aerosol dilution gas flow, in its present form, can not be suggested for spICP-MS analysis. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Principles and biophysical applications of single particle super-localization and rotational tracking

    Energy Technology Data Exchange (ETDEWEB)

    Gu, Yan [Iowa State Univ., Ames, IA (United States)

    2013-01-01

    While conventional Single Particle Tracking (SPT) techniques acquire 2D or 3D trajectories of particle probes, we have developed Single Particle Orientation and Rotational Tracking (SPORT) techniques to extract orientation and rotational information. Combined with DIC microscopy, the SPORT technique has been applied in biophysical studies, including membrane diffusion and intracellular transport. The rotational dynamics of nanoparticle vectors on live cell membranes was recorded and its influence on the fate of these nanoparticle vectors was elucidated. The rotational motions of gold nanorods with various surface modifiers were tracked continuously at a temporal resolution of 5 ms under a DIC microscope. We found that the rotational behaviors of gold nanorod vectors are strongly related to their surface charge, specific surface functional groups, and the availability of receptors on cell membranes. The study of rotational Brownian motion of nanoparticles on cell membranes will lead to a better understanding of the mechanisms of drug delivery and provide guidance in designing surface modification strategies for drug delivery vectors under various circumstances. To characterize the rotation mode of surface functionalized gold nanorods on cell membranes, the SPORT technique is combined with the correlation analysis of the bright and dark DIC intensities. The unique capabilities of visualizing and understanding rotational motions of functionalized nanoparticles on live cell membranes allow us to correlate rotational and translational dynamics in unprecedented detail and provide new insights for complex membrane processes, including electrostatic interactions, ligand-receptor binding, and lateral (confined and hopping) diffusion of membrane receptors. Surface-functionalized nanoparticles interact with the membrane in fundamentally different ways and exhibit distinct rotational modes. The early events of particle-membrane approach and attachment are directly visualized

  6. Characterisation of Black Carbon (BC) mixing state and flux in Beijing using single particle measurements.

    Science.gov (United States)

    Joshi, Rutambhara; Liu, Dantong; Allan, James; Coe, Hugh; Flynn, Michael; Broda, Kurtis; Olfert, Jason; Irwin, Martin; Sun, Yele; Fu, Pingqing; Wang, Junfeng; Ge, Xinlei; Langford, Ben; Nemitz, Eiko; Mullinger, Neil

    2017-04-01

    BC is generated by the incomplete combustion of carbonaceous fuels and it is an important component of fine PM2.5. In the atmosphere BC particles have a complex structure and its mixing state has crucial impact on optical properties. Quantifying the sources and emissions of black carbon in urban environments is important and presently uncertain, particularly in megacities undergoing rapid growth and change in emissions. During the winter of 2016 (10th Nov-10th Dec) the BC was characterised as part of a large joint UK-China field experiment in Beijing. This paper focuses on understanding the mixing state of BC as well as identification and quantification of BC sources. We used a combination of a Centrifugal Particle Mass Analyser (CPMA) and a Single Particle Soot Photometer (SP2) to uniquely quantify the morphology independent mass of single refractory BC particles and their coating content. The CPMA allows us to select pre-charged aerosol particles according to their mass to charge ratio and the SP2 provides information on the mass of refractory BC through a laser-induced incandescence method. Furthermore, another SP2 was used to measure the BC flux at 100m height using the Eddy Covariance method. We have successfully gathered 4 weeks of continuous measurements which include several severe pollution events in Beijing. Here we present preliminary results, characterising the distribution of coating mass on BC particles in Beijing and linking this to the main sources of BC in the city. We will provide initial estimates of the BC flux over a several kilometre footprint. Such analysis will provide important information for the further investigation of source distribution, emission, lifetime and optical properties of BC under complex environments in Beijing.

  7. RNA interference and single particle tracking analysis of hepatitis C virus endocytosis.

    Directory of Open Access Journals (Sweden)

    Kelly E Coller

    2009-12-01

    Full Text Available Hepatitis C virus (HCV enters hepatocytes following a complex set of receptor interactions, culminating in internalization via clathrin-mediated endocytosis. However, aside from receptors, little is known about the cellular molecular requirements for infectious HCV entry. Therefore, we analyzed a siRNA library that targets 140 cellular membrane trafficking genes to identify host genes required for infectious HCV production and HCV pseudoparticle entry. This approach identified 16 host cofactors of HCV entry that function primarily in clathrin-mediated endocytosis, including components of the clathrin endocytosis machinery, actin polymerization, receptor internalization and sorting, and endosomal acidification. We next developed single particle tracking analysis of highly infectious fluorescent HCV particles to examine the co-trafficking of HCV virions with cellular cofactors of endocytosis. We observe multiple, sequential interactions of HCV virions with the actin cytoskeleton, including retraction along filopodia, actin nucleation during internalization, and migration of internalized particles along actin stress fibers. HCV co-localizes with clathrin and the ubiquitin ligase c-Cbl prior to internalization. Entering HCV particles are associated with the receptor molecules CD81 and the tight junction protein, claudin-1; however, HCV-claudin-1 interactions were not restricted to Huh-7.5 cell-cell junctions. Surprisingly, HCV internalization generally occurred outside of Huh-7.5 cell-cell junctions, which may reflect the poorly polarized nature of current HCV cell culture models. Following internalization, HCV particles transport with GFP-Rab5a positive endosomes, which is consistent with trafficking to the early endosome. This study presents technical advances for imaging HCV entry, in addition to identifying new host cofactors of HCV infection, some of which may be antiviral targets.

  8. TrackMate: An open and extensible platform for single-particle tracking.

    Science.gov (United States)

    Tinevez, Jean-Yves; Perry, Nick; Schindelin, Johannes; Hoopes, Genevieve M; Reynolds, Gregory D; Laplantine, Emmanuel; Bednarek, Sebastian Y; Shorte, Spencer L; Eliceiri, Kevin W

    2017-02-15

    We present TrackMate, an open source Fiji plugin for the automated, semi-automated, and manual tracking of single-particles. It offers a versatile and modular solution that works out of the box for end users, through a simple and intuitive user interface. It is also easily scriptable and adaptable, operating equally well on 1D over time, 2D over time, 3D over time, or other single and multi-channel image variants. TrackMate provides several visualization and analysis tools that aid in assessing the relevance of results. The utility of TrackMate is further enhanced through its ability to be readily customized to meet specific tracking problems. TrackMate is an extensible platform where developers can easily write their own detection, particle linking, visualization or analysis algorithms within the TrackMate environment. This evolving framework provides researchers with the opportunity to quickly develop and optimize new algorithms based on existing TrackMate modules without the need of having to write de novo user interfaces, including visualization, analysis and exporting tools. The current capabilities of TrackMate are presented in the context of three different biological problems. First, we perform Caenorhabditis-elegans lineage analysis to assess how light-induced damage during imaging impairs its early development. Our TrackMate-based lineage analysis indicates the lack of a cell-specific light-sensitive mechanism. Second, we investigate the recruitment of NEMO (NF-κB essential modulator) clusters in fibroblasts after stimulation by the cytokine IL-1 and show that photodamage can generate artifacts in the shape of TrackMate characterized movements that confuse motility analysis. Finally, we validate the use of TrackMate for quantitative lifetime analysis of clathrin-mediated endocytosis in plant cells. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

  9. Overcoming challenges in single particle inductively coupled plasma mass spectrometry measurement of silver nanoparticles.

    Science.gov (United States)

    Liu, Jingyu; Murphy, Karen E; Winchester, Michael R; Hackley, Vincent A

    2017-10-01

    Single particle ICP-MS has evolved rapidly as a quantitative method for determining nanoparticle size and number concentration at environmentally relevant exposure levels. Central to the application of spICP-MS is a commonly used, but not rigorously validated, calibration approach based on the measured transport efficiency and the response of ionic standards. In this work, we present a comprehensive and systematic study of the accuracy, precision and robustness of spICP-MS using the rigorously characterized reference material (RM) 8017 (Polyvinylpyrrolidone Coated Nominal 75 nm Silver Nanoparticles), recently issued by the National Institute of Standards and Technology (NIST). We report for the first time, statistically significant differences in frequency-based and size-based measures of transport efficiency with NIST RM 8013 Gold Nanoparticles and demonstrate that the size-based measure of transport efficiency is more robust and yields accurate results for the silver nanoparticle RM relative to TEM-based reference values. This finding is significant, because the frequency-based method is more widely applied. Furthermore, we demonstrate that the use of acidified ionic standards improves measurement of ICP-MS Ag response, but does not degrade the accuracy of the results for AgNP suspensions in water or various other diluents. Approaches for controlling AgNP dissolution were investigated and are shown to effectively improve particle stability in dilute suspensions required for spICP-MS analysis, while minimally affecting the measured intensity and allowing for more robust analysis. This study is an important and necessary advancement toward full validation and adoption of spICP-MS by the broader research community. Graphical abstract Measurement challenges in spICP-MS analysis.

  10. FIREX-Related Biomass Burning Research Using ARM Single-Particle Soot Photometer Field Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    Onasch, Timothy B [Aerodyne Research, Inc.; Sedlacek, Arthur J [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2017-03-15

    The scientific focus of this study was to investigate and quantify the mass loadings, chemical compositions, and optical properties of biomass burning particulate emissions generated in the laboratory from Western U.S. fuels using a similar instrument suite to the one deployed on the U.S. Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) Climate Research Facility Gulfstream-1 (G-1) aircraft during the 2013 Biomass Burning Observation Project (BBOP) field study (Kleinman and Sedlacek, 2013). We deployed the single-particle soot photometer (SP2) to make measurements of biomass burning refractory black carbon (rBC) mass loadings and size distributions to correlate with non-refractory particulate matter (NR-PM; i.e., HR-AMS) and rBC (SP-AMS) measurements as a function of photo-oxidation processes in an environmental chamber. With these measurements, we will address the following scientific questions: 1. What are the emission indices (g/kg fuel) of rBC from various wildland fuels from the Pacific Northwest (i.e., relevant to BBOP analysis) as a function of combustion conditions and simulated atmospheric processing in an environmental chamber? 2. What are the optical properties (e.g., mass-specific absorption cross-section [MAC], single-scattering albedo [SSA], and absorption Angstrom exponent [AAE)] of rBC emitted from various wildland fuels and how are they impacted by atmospheric processing? 3. How does the mixing state of rBC in biomass-burning plumes relate to the optical properties? 4. How does the emitted rBC affect radiative forcing?

  11. Whimsicality of multi-mode Hasegawa space-charge waves in a complex plasma containing collision-dominated electrons and streaming ions

    Science.gov (United States)

    Lee, Myoung-Jae; Jung, Young-Dae

    2017-09-01

    The influence of collision-dominated electrons on multi-mode Hasegawa space-charge waves are investigated in a complex plasma containing streaming ions. The dispersion relation for the multi-mode Hasegawa space-charge wave propagating in a cylindrical waveguide filled with dusty plasma containing collision-dominated electrons and streaming ions is derived by using the fluid equations and Poisson’s equation which lead to a Bessel equation. By the boundary condition, the roots of the Bessel function would characterize the property of space-charge wave propagation. It is found that two solutions exist for wave frequency, which are affected by the radius of waveguide and the roots of the Bessel function. The damping and growing modes are found to be enhanced by an increase of the radius. However, an increase of electron collision frequency would suppress the damping and the growing modes of the propagating space-charge wave in a cylindrical waveguide plasma.

  12. An Efficient Genetic Agorithm for Solving the Multi-Mode Resource-Constrained Project Scheduling Problem Based on Random Key Representation

    Directory of Open Access Journals (Sweden)

    Mohammad Hassan Sebt

    2015-11-01

    Full Text Available In this paper, a new genetic algorithm (GA is presented for solving the multi-mode resource-constrained project scheduling problem (MRCPSP with minimization of project makespan as the objective subject to resource and precedence constraints. A random key and the related mode list (ML representation scheme are used as encoding schemes and the multi-mode serial schedule generation scheme (MSSGS is considered as the decoding procedure. In this paper, a simple, efficient fitness function is proposed which has better performance compared to the other fitness functions in the literature. Defining a new mutation operator for ML is the other contribution of the current study. Comparing the results of the proposed GA with other approaches using the well-known benchmark sets in PSPLIB validates the effectiveness of the proposed algorithm to solve the MRCPSP.

  13. Role of multi-mode ultrasound in the diagnosis of level 4 BI-RADS breast lesions and Logistic regression model.

    Science.gov (United States)

    Leng, Xiaoling; Huang, Guofu; Yao, Lanhui; Ma, Fucheng

    2015-01-01

    This study is to investigate the diagnostic role of multi-mode ultrasound in level 4 BI-RADS breast lesions and to establish a Logistic regression model. Totally 179 patients with 182 sites of breast lesions were enrolled in this study. Preoperatively, the examinations of routine ultrasonography, elastography, contrast-enhanced ultrasonography and three-dimensional color Doppler were performed. Postoperatively, the breast lesions were diagnosed as benign and malignant lesions according to pathological results. Diagnostic indicators of each ultrasound analysis were determined and compared. The relationship between these diagnostic indicators and the benign and malignant features of breast lesions was analyzed by single factor analysis. Logistic regression model was established. The diagnostic indicators with high sensitivity and specificity were tumor edge, enhanced range and score of elastography. Four factors of tumor edge, enhanced order, contrast mode and score of elastography were related with the benign and malignant features of breast lesions. The prediction model was Logit (P) = 0.636 + 4.471X1 + 4.337X2 + 3.753X3 + 3.014X4 + 2.525X5 + 2.105X6. Likelihood ratio test showed that the model was statistically significant (χ(2) = 161.876, P R(2) = 0.813, prediction accuracy 92.3%). The differences in sensitivity and specificity between multi-mode ultrasound diagnosis and routine ultrasound diagnosis were statistically significant (P Logistic regression model and multi-mode ultrasound diagnosis. Multi-mode ultrasound and Logistic regression model are more effective in diagnosing level 4 BI-RADS breast lesions.

  14. Development of a numerical tool to study the mixing phenomenon occurring during mode one operation of a multi-mode ejector-augmented pulsed detonation rocket engine

    Science.gov (United States)

    Dawson, Joshua

    A novel multi-mode implementation of a pulsed detonation engine, put forth by Wilson et al., consists of four modes; each specifically designed to capitalize on flow features unique to the various flow regimes. This design enables the propulsion system to generate thrust through the entire flow regime. The Multi-Mode Ejector-Augmented Pulsed Detonation Rocket Engine operates in mode one during take-off conditions through the acceleration to supersonic speeds. Once the mixing chamber internal flow exceeds supersonic speed, the propulsion system transitions to mode two. While operating in mode two, supersonic air is compressed in the mixing chamber by an upstream propagating detonation wave and then exhausted through the convergent-divergent nozzle. Once the velocity of the air flow within the mixing chamber exceeds the Chapman-Jouguet Mach number, the upstream propagating detonation wave no longer has sufficient energy to propagate upstream and consequently the propulsive system shifts to mode three. As a result of the inability of the detonation wave to propagate upstream, a steady oblique shock system is established just upstream of the convergent-divergent nozzle to initiate combustion. And finally, the propulsion system progresses on to mode four operation, consisting purely of a pulsed detonation rocket for high Mach number flight and use in the upper atmosphere as is needed for orbital insertion. Modes three and four appear to be a fairly significant challenge to implement, while the challenge of implementing modes one and two may prove to be a more practical goal in the near future. A vast number of potential applications exist for a propulsion system that would utilize modes one and two, namely a high Mach number hypersonic cruise vehicle. There is particular interest in the dynamics of mode one operation, which is the subject of this research paper. Several advantages can be obtained by use of this technology. Geometrically the propulsion system is fairly

  15. Silver speciation and characterization of nanoparticles released from plastic food containers by single particle ICPMS.

    Science.gov (United States)

    Ramos, K; Gómez-Gómez, M M; Cámara, C; Ramos, L

    2016-05-01

    Silver migration from a commercial baby feeding bottle and a food box containing AgNPs, as confirmed by SEM-EDX analysis, was evaluated using food simulant solutions [i.e., water, 3% (v/v) acetic acid, and 10% and 90% (v/v) ethanol]. Silver release was investigated at temperatures in the 20-70°C range using contact times of up to 10 days. Migration of silver from the food box was in all cases 2 to 3 orders of magnitude higher than that observed for the baby bottle, although the total silver content in the original box material was half of that found in the baby bottle. As expected, for both food containers, silver migration depended on both the nature of the tested solution and the applied conditions. The highest release was observed for 3% acetic acid at 70°C for 2h, corresponding to 62ngdm(2) and 1887ngdm(-2) of silver for the baby bottle and the food box, respectively. Single particle-inductively coupled plasma mass spectrometry (SP-ICPMS) was used to characterise and quantify AgNPs in the food simulants extracts. Sample preparation was optimized to preserve AgNPs integrity. The experimental parameters affecting AgNPs detection, sizing and quantification by SP-ICPMS were also optimised. Analyses of water and acidic extracts revealed the presence of both dissolved silver and AgNPs. Small AgNPs (in the 18-30nm range) and particle number concentrations within the 4-1510 10(6)L(-1) range were detected, corresponding to only 0.1-8.6% of the total silver released from these materials. The only exception was AgNPs migrated into water at 40°C and 70°C from the food box, which accounted for as much as 34% and 69% of the total silver content, respectively. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. Measuring the temporal evolution of aerosol composition in a remote marine environment influenced by Saharan dust outflow using a new single particle mass spectrometer.

    Science.gov (United States)

    Marsden, Nicholas; Williams, Paul; Flynn, Michael; Taylor, Jonathan; Liu, Dantong; Allan, James; Coe, Hugh

    2016-04-01

    Refractory material constitutes a significant fraction of the atmospheric aerosol burden and has a strong influence on climate through the direct radiative effect and aerosol-cloud interactions, particularly in cold and mixed phase clouds. Composition of refractory aerosols is traditionally measured using off-line analytical techniques such as filter analyses. However, when using off-line techniques the temporal evolution of the data set is lost, meaning the measurements are difficult to relate to atmospheric processes. Recently, single particle mass spectrometry (SPMS) has proven a useful tool for the on-line study of refractory aerosols with the ability to probe size resolved chemical composition with high temporal resolution on a particle by particle basis. A new Laser Ablation Aerosol Time-of-Flight (LAAP-TOF) SPMS instrument with a modified optical detection system was deployed for ground based measurements at Praia, Cape Verde during the Ice in Cloud - Dust (ICE-D) multi-platform campaign in August 2015. A primary aim of the project was to evaluate the impact of Saharan dust on ice nucleation in mixed phase clouds. The instrument was operated over a 16 day period in which several hundred thousand single particle mass spectra were obtained from air masses with back trajectories traversing the Mid-Atlantic, Sahara Desert and West Africa. The data presented indicate external mixtures of sea salt and silicate mineral dust internally mixed with secondary species that are consistent with long range transport to a remote marine environment. The composition and size distributions measured with the LAAP-TOF are compared with measurements from an aerodynamic particle sizer (APS), Single Particle Soot Photometer (SP2), and data from SEM-EDX analysis of filter samples. The particle number fraction identified as silicate mineral from the mass spectra correlates with a fraction of the incandescent particles measured with the SP2. We discuss the suitability of the modified

  17. Two-Step Single Particle Mass Spectrometry for On-Line Monitoring of Polycyclic Aromatic Hydrocarbons Bound to Ambient Fine Particulate Matter

    Science.gov (United States)

    Zimmermann, R.; Bente, M.; Sklorz, M.

    2007-12-01

    Polycyclic aromatic hydrocarbons (PAH) are formed as trace products in combustion processes and are emitted to the atmosphere. Larger PAH have low vapour pressure and are predominantly bound to the ambient fine particulate matter (PM). Upon inhalation, PAH show both, chronic human toxicity (i.e. many PAH are potent carcinogens) as well as acute human toxicity (i.e. inflammatory effects due to oxi-dative stress) and are discussed to be relevant for the observed health effect of ambient PM. Therefore a better understanding of the occurrence, dynamics and particle size dependence of particle bound-PAH is of great interest. On-line aerosol mass spectrometry in principle is the method of choice to investigate the size resolved changes in the chemical speciation of particles as well the status of internal vs. external mixing of chemical constituents. However the present available aerosol mass spectrometers (ATOFMS and AMS) do not allow detection of PAH from ambient air PM. In order to allow a single particle based monitoring of PAH from ambient PM a new single particle laser ionisation mass spectrometer was built and applied. The system is based on ATOFMS principle but uses a two- step photo-ionization. A tracked and sized particle firstly is laser desorbed (LD) by a IR-laser pulse (CO2-laser, λ=10.2 μm) and subsequently the released PAH are selectively ionized by an intense UV-laser pulse (ArF excimer, λ=248 nm) in a resonance enhanced multiphoton ionisation process (REMPI). The PAH-ions are detected in a time of flight mass spectrometer (TOFMS). A virtual impactor enrichment unit is used to increase the detection frequency of the ambient particles. With the current inlet system particles from about 400 nm to 10 μm are accessible. Single particle based temporal profiles of PAH containing particles ion (size distribution and PAH speciation) have been recorded in Oberschleissheim, Germany from ambient air. Furthermore profiles of relevant emission sources (e

  18. Convergence of lateral dynamic measurements in the plasma membrane of live cells from single particle tracking and STED-FCS

    DEFF Research Database (Denmark)

    Lagerholm, B. Christoffer; Andrade, Débora M.; Clausen, Mathias P.

    2017-01-01

    Fluorescence correlation spectroscopy (FCS) in combination with the super-resolution imaging method STED (STED-FCS), and single-particle tracking (SPT) are able to directly probe the lateral dynamics of lipids and proteins in the plasma membrane of live cells at spatial scales much below the diff......Fluorescence correlation spectroscopy (FCS) in combination with the super-resolution imaging method STED (STED-FCS), and single-particle tracking (SPT) are able to directly probe the lateral dynamics of lipids and proteins in the plasma membrane of live cells at spatial scales much below...... the diffraction limit of conventional microscopy. However, a major disparity in interpretation of data from SPT and STED-FCS remains, namely the proposed existence of a very fast (unhindered) lateral diffusion coefficient, ≥5 μm2 s-1, in the plasma membrane of live cells at very short length scales, ≈ 100 nm...

  19. Determination of the ribosome structure to a resolution of 2.5 Å by single-particle cryo-EM.

    Science.gov (United States)

    Liu, Zheng; Gutierrez-Vargas, Cristina; Wei, Jia; Grassucci, Robert A; Sun, Ming; Espina, Noel; Madison-Antenucci, Susan; Tong, Liang; Frank, Joachim

    2017-01-01

    With the advance of new instruments and algorithms, and the accumulation of experience over decades, single-particle cryo-EM has become a pivotal part of structural biology. Recently, we determined the structure of a eukaryotic ribosome at 2.5 Å for the large subunit. The ribosome was derived from Trypanosoma cruzi, the protozoan pathogen of Chagas disease. The high-resolution density map allowed us to discern a large number of unprecedented details including rRNA modifications, water molecules, and ions such as Mg 2+ and Zn 2+ . In this paper, we focus on the procedures for data collection, image processing, and modeling, with particular emphasis on factors that contributed to the attainment of high resolution. The methods described here are readily applicable to other macromolecules for high-resolution reconstruction by single-particle cryo-EM. © 2016 The Protein Society.

  20. Quasifree (p , 2 p ) Reactions on Oxygen Isotopes: Observation of Isospin Independence of the Reduced Single-Particle Strength

    Science.gov (United States)

    Atar, L.; Paschalis, S.; Barbieri, C.; Bertulani, C. A.; Díaz Fernández, P.; Holl, M.; Najafi, M. A.; Panin, V.; Alvarez-Pol, H.; Aumann, T.; Avdeichikov, V.; Beceiro-Novo, S.; Bemmerer, D.; Benlliure, J.; Boillos, J. M.; Boretzky, K.; Borge, M. J. G.; Caamaño, M.; Caesar, C.; Casarejos, E.; Catford, W.; Cederkall, J.; Chartier, M.; Chulkov, L.; Cortina-Gil, D.; Cravo, E.; Crespo, R.; Dillmann, I.; Elekes, Z.; Enders, J.; Ershova, O.; Estrade, A.; Farinon, F.; Fraile, L. M.; Freer, M.; Galaviz Redondo, D.; Geissel, H.; Gernhäuser, R.; Golubev, P.; Göbel, K.; Hagdahl, J.; Heftrich, T.; Heil, M.; Heine, M.; Heinz, A.; Henriques, A.; Hufnagel, A.; Ignatov, A.; Johansson, H. T.; Jonson, B.; Kahlbow, J.; Kalantar-Nayestanaki, N.; Kanungo, R.; Kelic-Heil, A.; Knyazev, A.; Kröll, T.; Kurz, N.; Labiche, M.; Langer, C.; Le Bleis, T.; Lemmon, R.; Lindberg, S.; Machado, J.; Marganiec-Gałązka, J.; Movsesyan, A.; Nacher, E.; Nikolskii, E. Y.; Nilsson, T.; Nociforo, C.; Perea, A.; Petri, M.; Pietri, S.; Plag, R.; Reifarth, R.; Ribeiro, G.; Rigollet, C.; Rossi, D. M.; Röder, M.; Savran, D.; Scheit, H.; Simon, H.; Sorlin, O.; Syndikus, I.; Taylor, J. T.; Tengblad, O.; Thies, R.; Togano, Y.; Vandebrouck, M.; Velho, P.; Volkov, V.; Wagner, A.; Wamers, F.; Weick, H.; Wheldon, C.; Wilson, G. L.; Winfield, J. S.; Woods, P.; Yakorev, D.; Zhukov, M.; Zilges, A.; Zuber, K.; R3B Collaboration

    2018-01-01

    Quasifree one-proton knockout reactions have been employed in inverse kinematics for a systematic study of the structure of stable and exotic oxygen isotopes at the R3B /LAND setup with incident beam energies in the range of 300 - 450 MeV /u . The oxygen isotopic chain offers a large variation of separation energies that allows for a quantitative understanding of single-particle strength with changing isospin asymmetry. Quasifree knockout reactions provide a complementary approach to intermediate-energy one-nucleon removal reactions. Inclusive cross sections for quasifree knockout reactions of the type O A (p ,2 p )N-1A have been determined and compared to calculations based on the eikonal reaction theory. The reduction factors for the single-particle strength with respect to the independent-particle model were obtained and compared to state-of-the-art ab initio predictions. The results do not show any significant dependence on proton-neutron asymmetry.

  1. Inclusive single-particle production in two-photon collisions at LEP II with the DELPHI detector

    CERN Document Server

    Abdallah, J.; Adam, W.; Adzic, P.; Albrecht, T.; Alemany-Fernandez, R.; Allmendinger, T.; Allport, P.P.; Amaldi, U.; Amapane, N.; Amato, S.; Anashkin, E.; Andreazza, A.; Andringa, S.; Anjos, N.; Antilogus, P.; Apel, W.D.; Arnoud, Y.; Ask, S.; Asman, B.; Augustin, J.E.; Augustinus, A.; Baillon, P.; Ballestrero, A.; Bambade, P.; Barbier, R.; Bardin, D.; Barker, G.J.; Baroncelli, A.; Battaglia, M.; Baubillier, M.; Becks, K.H.; Begalli, M.; Behrmann, A.; Ben-Haim, E.; Benekos, N.; Benvenuti, A.; Berat, C.; Berggren, M.; Bertrand, D.; Besancon, M.; Besson, N.; Bloch, D.; Blom, M.; Bluj, M.; Bonesini, M.; Boonekamp, M.; Booth, P.S.L.; Borisov, G.; Botner, O.; Bouquet, B.; Bowcock, T.J.V.; Boyko, I.; Bracko, M.; Brenner, R.; Brodet, E.; Bruckman, P.; Brunet, J.M.; Buschbeck, B.; Buschmann, P.; Calvi, M.; Camporesi, T.; Canale, V.; Carena, F.; Castro, N.; Cavallo, F.; Chapkin, M.; Charpentier, Ph.; Checchia, P.; Chierici, R.; Chliapnikov, P.; Chudoba, J.; Chung, S.U.; Cieslik, K.; Collins, P.; Contri, R.; Cosme, G.; Cossutti, F.; Costa, M.J.; Crennell, D.; Cuevas, J.; D'Hondt, J.; da Silva, T.; Da Silva, W.; Della Ricca, G.; De Angelis, A.; De Boer, W.; De Clercq, C.; De Lotto, B.; De Maria, N.; De Min, A.; de Paula, L.; Di Ciaccio, L.; Di Simone, A.; Doroba, K.; Drees, J.; Eigen, G.; Ekelof, T.; Ellert, M.; Elsing, M.; Espirito Santo, M.C.; Fanourakis, G.; Fassouliotis, D.; Feindt, M.; Fernandez, J.; Ferrer, A.; Ferro, F.; Flagmeyer, U.; Foeth, H.; Fokitis, E.; Fulda-Quenzer, F.; Fuster, J.; Gandelman, M.; Garcia, C.; Gavillet, Ph.; Gazis, E.; Gokieli, R.; Golob, B.; Gomez-Ceballos, G.; Goncalves, P.; Graziani, E.; Grosdidier, G.; Grzelak, K.; Guy, J.; Haag, C.; Hallgren, A.; Hamacher, K.; Hamilton, K.; Haug, S.; Hauler, F.; Hedberg, V.; Hennecke, M.; Hoffman, J.; Holmgren, S.O.; Holt, P.J.; Houlden, M.A.; Jackson, J.N.; Jarlskog, G.; Jarry, P.; Jeans, D.; Johansson, E.K.; Jonsson, P.; Joram, C.; Jungermann, L.; Kapusta, F.; Katsanevas, S.; Katsoufis, E.; Kernel, G.; Kersevan, B.P.; Kerzel, U.; King, B.T.; Kjaer, N.J.; Kluit, P.; Kokkinias, P.; Kourkoumelis, C.; Kouznetsov, O.; Krumstein, Z.; Kucharczyk, M.; Lamsa, J.; Leder, G.; Ledroit, F.; Leinonen, L.; Leitner, R.; Lemonne, J.; Lepeltier, V.; Lesiak, T.; Liebig, W.; Liko, D.; Lipniacka, A.; Lopes, J.H.; Lopez, J.M.; Loukas, D.; Lutz, P.; Lyons, L.; MacNaughton, J.; Malek, A.; Maltezos, S.; Mandl, F.; Marco, J.; Marco, R.; Marechal, B.; Margoni, M.; Marin, J.C.; Mariotti, C.; Markou, A.; Martinez-Rivero, C.; Masik, J.; Mastroyiannopoulos, N.; Matorras, F.; Matteuzzi, C.; Mazzucato, F.; Mazzucato, M.; Mc Nulty, R.; Meroni, C.; Migliore, E.; Mitaroff, W.; Mjoernmark, U.; Moa, T.; Moch, M.; Moenig, K.; Monge, R.; Montenegro, J.; Moraes, D.; Moreno, S.; Morettini, P.; Mueller, U.; Muenich, K.; Mulders, M.; Mundim, L.; Murray, W.; Muryn, B.; Myatt, G.; Myklebust, T.; Nassiakou, M.; Navarria, F.; Nawrocki, K.; Nemecek, S.; Nicolaidou, R.; Nikolenko, M.; Oblakowska-Mucha, A.; Obraztsov, V.; Olshevski, A.; Onofre, A.; Orava, R.; Osterberg, K.; Ouraou, A.; Oyanguren, A.; Paganoni, M.; Paiano, S.; Palacios, J.P.; Palka, H.; Papadopoulou, Th.D.; Pape, L.; Parkes, C.; Parodi, F.; Parzefall, U.; Passeri, A.; Passon, O.; Peralta, L.; Perepelitsa, V.; Perrotta, A.; Petrolini, A.; Piedra, J.; Pieri, L.; Pierre, F.; Pimenta, M.; Piotto, E.; Podobnik, T.; Poireau, V.; Pol, M.E.; Polok, G.; Pozdniakov, V.; Pukhaeva, N.; Pullia, A.; Radojicic, D.; Rebecchi, P.; Rehn, J.; Reid, D.; Reinhardt, R.; Renton, P.; Richard, F.; Ridky, J.; Rivero, M.; Rodriguez, D.; Romero, A.; Ronchese, P.; Roudeau, P.; Rovelli, T.; Ruhlmann-Kleider, V.; Ryabtchikov, D.; Sadovsky, A.; Salmi, L.; Salt, J.; Sander, C.; Savoy-Navarro, A.; Schwickerath, U.; Sekulin, R.; Siebel, M.; Sisakian, A.; Smadja, G.; Smirnova, O.; Sokolov, A.; Sopczak, A.; Sosnowski, R.; Spassov, T.; Stanitzki, M.; Stocchi, A.; Strauss, J.; Stugu, B.; Szczekowski, M.; Szeptycka, M.; Szumlak, T.; Tabarelli, T.; Tegenfeldt, F.; Timmermans, J.; Tkatchev, L.; Tobin, M.; Todorovova, S.; Tome, B.; Tonazzo, A.; Tortosa, P.; Travnicek, P.; Treille, D.; Tristram, G.; Trochimczuk, M.; Troncon, C.; Turluer, M.L.; Tyapkin, I.A.; Tyapkin, P.; Tzamarias, S.; Uvarov, V.; Valenti, G.; Van Dam, P.; Van Eldik, J.; van Remortel, N.; Van Vulpen, I.; Vegni, G.; Veloso, F.; Venus, W.; Verdier, P.; Verzi, V.; Vilanova, D.; Vitale, L.; Vrba, V.; Wahlen, H.; Washbrook, A.J.; Weiser, C.; Wicke, D.; Wickens, J.; Wilkinson, G.; Winter, M.; Witek, M.; Yushchenko, O.; Zalewska, A.; Zalewski, P.; Zavrtanik, D.; Zhuravlov, V.; Zimine, N.I.; Zintchenko, A.; Zupan, M.

    2009-01-01

    A study of the inclusive charged hadron production in two-photon collisions is described. The data were collected with the DELPHI detector at LEP II. Results on the inclusive single-particle p_T distribution and the differential charged hadrons dsigma/dp_T cross-section are presented and compared to the predictions of perturbative NLO QCD calculations and to published results.

  2. Mass spectra features of biomass burning boiler and coal burning boiler emitted particles by single particle aerosol mass spectrometer.

    Science.gov (United States)

    Xu, Jiao; Li, Mei; Shi, Guoliang; Wang, Haiting; Ma, Xian; Wu, Jianhui; Shi, Xurong; Feng, Yinchang

    2017-11-15

    In this study, single particle mass spectra signatures of both coal burning boiler and biomass burning boiler emitted particles were studied. Particle samples were suspended in clean Resuspension Chamber, and analyzed by ELPI and SPAMS simultaneously. The size distribution of BBB (biomass burning boiler sample) and CBB (coal burning boiler sample) are different, as BBB peaks at smaller size, and CBB peaks at larger size. Mass spectra signatures of two samples were studied by analyzing the average mass spectrum of each particle cluster extracted by ART-2a in different size ranges. In conclusion, BBB sample mostly consists of OC and EC containing particles, and a small fraction of K-rich particles in the size range of 0.2-0.5μm. In 0.5-1.0μm, BBB sample consists of EC, OC, K-rich and Al_Silicate containing particles; CBB sample consists of EC, ECOC containing particles, while Al_Silicate (including Al_Ca_Ti_Silicate, Al_Ti_Silicate, Al_Silicate) containing particles got higher fractions as size increase. The similarity of single particle mass spectrum signatures between two samples were studied by analyzing the dot product, results indicated that part of the single particle mass spectra of two samples in the same size range are similar, which bring challenge to the future source apportionment activity by using single particle aerosol mass spectrometer. Results of this study will provide physicochemical information of important sources which contribute to particle pollution, and will support source apportionment activities. Copyright © 2017. Published by Elsevier B.V.

  3. A local-optimization refinement algorithm in single particle analysis for macromolecular complex with multiple rigid modules

    Directory of Open Access Journals (Sweden)

    Hong Shan

    2015-12-01

    Full Text Available ABSTRACT Single particle analysis, which can be regarded as an average of signals from thousands or even millions of particle projections, is an efficient method to study the three-dimensional structures of biological macromolecules. An intrinsic assumption in single particle analysis is that all the analyzed particles must have identical composition and conformation. Thus specimen heterogeneity in either composition or conformation has raised great challenges for high-resolution analysis. For particles with multiple conformations, inaccurate alignments and orientation parameters will yield an averaged map with diminished resolution and smeared density. Besides extensive classification approaches, here based on the assumption that the macromolecular complex is made up of multiple rigid modules whose relative orientations and positions are in slight fluctuation around equilibriums, we propose a new method called as local optimization refinement to address this conformational heterogeneity for an improved resolution. The key idea is to optimize the orientation and shift parameters of each rigid module and then reconstruct their three-dimensional structures individually. Using simulated data of 80S/70S ribosomes with relative fluctuations between the large (60S/50S and the small (40S/30S subunits, we tested this algorithm and found that the resolutions of both subunits are significantly improved. Our method provides a proof-of-principle solution for high-resolution single particle analysis of macromolecular complexes with dynamic conformations.

  4. A local-optimization refinement algorithm in single particle analysis for macromolecular complex with multiple rigid modules.

    Science.gov (United States)

    Shan, Hong; Wang, Zihao; Zhang, Fa; Xiong, Yong; Yin, Chang-Cheng; Sun, Fei

    2016-01-01

    Single particle analysis, which can be regarded as an average of signals from thousands or even millions of particle projections, is an efficient method to study the three-dimensional structures of biological macromolecules. An intrinsic assumption in single particle analysis is that all the analyzed particles must have identical composition and conformation. Thus specimen heterogeneity in either composition or conformation has raised great challenges for high-resolution analysis. For particles with multiple conformations, inaccurate alignments and orientation parameters will yield an averaged map with diminished resolution and smeared density. Besides extensive classification approaches, here based on the assumption that the macromolecular complex is made up of multiple rigid modules whose relative orientations and positions are in slight fluctuation around equilibriums, we propose a new method called as local optimization refinement to address this conformational heterogeneity for an improved resolution. The key idea is to optimize the orientation and shift parameters of each rigid module and then reconstruct their three-dimensional structures individually. Using simulated data of 80S/70S ribosomes with relative fluctuations between the large (60S/50S) and the small (40S/30S) subunits, we tested this algorithm and found that the resolutions of both subunits are significantly improved. Our method provides a proof-of-principle solution for high-resolution single particle analysis of macromolecular complexes with dynamic conformations.

  5. A tilt-pair based method for assigning the projection directions of randomly oriented single-particle molecules.

    Science.gov (United States)

    Ueno, Yutaka; Mine, Shouhei; Kawasaki, Kazunori

    2015-04-01

    In this article, we describe an improved method to assign the projection angle for averaged images using tilt-pair images for three-dimensional reconstructions from randomly oriented single-particle molecular images. Our study addressed the so-called 'initial volume problem' in the single-particle reconstruction, which involves estimation of projection angles of the particle images. The projected images of the particles in different tilt observations were mixed and averaged for the characteristic views. After the ranking of these group average images in terms of reliable tilt angle information, mutual tilt angles between images are assigned from the constituent tilt-pair information. Then, multiples of the conical tilt series are made and merged to construct a network graph of the particle images in terms of projection angles, which are optimized for the three-dimensional reconstruction. We developed the method with images of a synthetic object and applied it to a single-particle image data set of the purified deacetylase from archaea. With the introduction of low-angle tilt observations to minimize unfavorable imaging conditions due to tilting, the results demonstrated reasonable reconstruction models without imposing symmetry to the structure. This method also guides its users to discriminate particle images of different conformational state of the molecule. © The Author 2015. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  6. Effect of Structural Heterogeneity in Chemical Composition on Online Single-Particle Mass Spectrometry Analysis of Sea Spray Aerosol Particles.

    Science.gov (United States)

    Sultana, Camille M; Collins, Douglas B; Prather, Kimberly A

    2017-04-04

    Knowledge of the surface composition of sea spray aerosols (SSA) is critical for understanding and predicting climate-relevant impacts. Offline microscopy and spectroscopy studies have shown that dry supermicron SSA tend to be spatially heterogeneous particles with sodium- and chloride-rich cores surrounded by organic enriched surface layers containing minor inorganic seawater components such as magnesium and calcium. At the same time, single-particle mass spectrometry reveals several different mass spectral ion patterns, suggesting that there may be a number of chemically distinct particle types. This study investigates factors controlling single particle mass spectra of nascent supermicron SSA. Depth profiling experiments conducted on SSA generated by a fritted bubbler and total ion intensity analysis of SSA generated by a marine aerosol reference tank were compared with observations of ambient SSA observed at two coastal locations. Analysis of SSA produced by utilizing controlled laboratory methods reveals that single-particle mass spectra with weak sodium ion signals can be produced by the desorption of the surface of typical dry SSA particles composed of salt cores and organic-rich coatings. Thus, this lab-based study for the first time unifies findings from offline and online measurements as well as lab and field studies of the SSA particle-mixing state.

  7. Facilitating model reconstruction for single-particle scattering using small-angle X-ray scattering methods.

    Science.gov (United States)

    Ma, Shufen; Liu, Haiguang

    2016-04-01

    X-ray free-electron lasers generate intense femtosecond X-ray pulses, so that high-resolution structure determination becomes feasible from noncrystalline samples, such as single particles or single molecules. At the moment, the orientation of sample particles cannot be precisely controlled, and consequently the unknown orientation needs to be recovered using computational algorithms. This delays the model reconstruction until all the scattering patterns have been re-oriented, which often entails a long elapse of time and until the completion of the experiment. The scattering patterns from single particles or multiple particles can be summed to form a virtual powder diffraction pattern, and the low-resolution region, corresponding to the small-angle X-ray scattering (SAXS) regime, can be analysed using existing SAXS methods. This work presents a pipeline that converts single-particle data sets into SAXS data, from which real-time model reconstruction is achieved using the model retrieval approach implemented in the software package SASTBX [Liu, Hexemer & Zwart (2012). J. Appl. Cryst. 45 , 587-593]. To illustrate the applications, two case studies are presented with real experimental data sets collected at the Linac Coherent Light Source.

  8. Single-Particle Cryo-EM and 3D Reconstruction of Hybrid Nanoparticles with Electron-Dense Components.

    Science.gov (United States)

    Yu, Guimei; Yan, Rui; Zhang, Chuan; Mao, Chengde; Jiang, Wen

    2015-10-01

    Single-particle cryo-electron microscopy (cryo-EM), accompanied with 3D reconstruction, is a broadly applicable tool for the structural characterization of macromolecules and nanoparticles. Recently, the cryo-EM field has pushed the limits of this technique to higher resolutions and samples of smaller molecular mass, however, some samples still present hurdles to this technique. Hybrid particles with electron-dense components, which have been studied using single-particle cryo-EM yet with limited success in 3D reconstruction due to the interference caused by electron-dense elements, constitute one group of such challenging samples. To process such hybrid particles, a masking method is developed in this work to adaptively remove pixels arising from electron-dense portions in individual projection images while maintaining maximal biomass signals for subsequent 2D alignment, 3D reconstruction, and iterative refinements. As demonstrated by the success in 3D reconstruction of an octahedron DNA/gold hybrid particle, which has been previously published without a 3D reconstruction, the devised strategy that combines adaptive masking and standard single-particle 3D reconstruction approach has overcome the hurdle of electron-dense elements interference, and is generally applicable to cryo-EM structural characterization of most, if not all, hybrid nanomaterials with electron-dense components. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Development of a high throughput single-particle screening for inorganic semiconductor nanorods as neural voltage sensor

    Science.gov (United States)

    Kuo, Yung; Park, Kyoungwon; Li, Jack; Ingargiola, Antonino; Park, Joonhyuck; Shvadchak, Volodymyr; Weiss, Shimon

    2017-08-01

    Monitoring membrane potential in neurons requires sensors with minimal invasiveness, high spatial and temporal (sub-ms) resolution, and large sensitivity for enabling detection of sub-threshold activities. While organic dyes and fluorescent proteins have been developed to possess voltage-sensing properties, photobleaching, cytotoxicity, low sensitivity, and low spatial resolution have obstructed further studies. Semiconductor nanoparticles (NPs), as prospective voltage sensors, have shown excellent sensitivity based on Quantum confined Stark effect (QCSE) at room temperature and at single particle level. Both theory and experiment have shown their voltage sensitivity can be increased significantly via material, bandgap, and structural engineering. Based on theoretical calculations, we synthesized one of the optimal candidates for voltage sensors: 12 nm type-II ZnSe/CdS nanorods (NRs), with an asymmetrically located seed. The voltage sensitivity and spectral shift were characterized in vitro using spectrally-resolved microscopy using electrodes grown by thin film deposition, which "sandwich" the NRs. We characterized multiple batches of such NRs and iteratively modified the synthesis to achieve higher voltage sensitivity (ΔF/F> 10%), larger spectral shift (>5 nm), better homogeneity, and better colloidal stability. Using a high throughput screening method, we were able to compare the voltage sensitivity of our NRs with commercial spherical quantum dots (QDs) with single particle statistics. Our method of high throughput screening with spectrally-resolved microscope also provides a versatile tool for studying single particles spectroscopy under field modulation.

  10. The single-particle density of states, bound states, phase-shift flip, and a resonance in the presence of an Aharonov-Bohm potential

    International Nuclear Information System (INIS)

    Moroz, A.

    1994-01-01

    Both the nonrelativistic scattering and the spectrum in the presence of the Aharonov-Bohm potential are analyzed, and the single-particle density of states for different self-adjoint extensions is calculated, which is shown to be a symmetric and periodic function of the flux depending only on the distance from the nearest integer. The Aharonov-Casher theorem on the number of zero modes is corrected for the singular field configuration. The Hall resistivity is calculated in the dilute vortex limit. The magnetic moment coupling and not the spin is shown to be the primary source for the phase-shift flip that may occur even in its absence. The total energy of the system consisting of particles and field is discussed. (author) 65 refs.; 5 figs.; 1 tab

  11. Detection and characterization of silver nanoparticles in chicken meat by asymmetric flow field flow fractionation with detection by conventional or single particle ICP-MS

    DEFF Research Database (Denmark)

    Löschner, Katrin; Navratilova, Jana; Købler, Carsten

    2013-01-01

    A method of analysis of silver nanoparticles (AgNPs) in chicken meat was developed. The homogenized chicken meat sample, which was spiked with AgNPs, was subjected to enzymolysis by Proteinase K for 40 min at 37 °C. Transmission electron microscopy and inductively coupled plasma mass spectrometry...... (ICP-MS) in single particle mode were used to characterize the number-based size distribution of AgNPs in the meat digestate. Because similar size distributions were found in the meat digestate and in the aqueous suspension of AgNPs used for spiking the meat, it was shown that no detectable dissolution...... of the AgNPs took place during the sample preparation stage. The digestate was injected into the asymmetric flow field flow fractionation (AF(4)) -ICP-MS system, which enabled fractionation of nanoparticles from the remaining meat matrix, and resulted in one large peak in the fractograms as well as two...

  12. Practical limitations of single particle ICP-MS in the determination of nanoparticle size distributions and dissolution: case of rare earth oxides.

    Science.gov (United States)

    Fréchette-Viens, Laurie; Hadioui, Madjid; Wilkinson, Kevin J

    2017-01-15

    The applicability of single particle ICP-MS (SP-ICP-MS) for the analysis of nanoparticle size distributions and the determination of particle numbers was evaluated using the rare earth oxide, La 2 O 3 , as a model particle. The composition of the storage containers, as well as the ICP-MS sample introduction system were found to significantly impact SP-ICP-MS analysis. While La 2 O 3 nanoparticles (La 2 O 3 NP) did not appear to interact strongly with sample containers, adsorptive losses of La 3+ (over 24h) were substantial (>72%) for fluorinated ethylene propylene bottles as opposed to polypropylene (ICP-MS may nonetheless be appropriate for NP analysis in environmental matrices. Coupling of an ion-exchange resin to the SP-ICP-MS led to more accurate determinations of the La 2 O 3 NP size distributions. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. Intracellular formation of α-synuclein oligomers and the effect of heat shock protein 70 characterized by confocal single particle spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Levin, Johannes [Department of Neurology, Ludwig-Maximilians-University, Marchioninistr. 15, 81377 Munich (Germany); German Center for Neurodegenerative Diseases – DZNE, Site Munich, Feodor-Lynen-Str. 17, 81377 Munich (Germany); Hillmer, Andreas S. [Center for Neuropathology and Prion Research, Ludwig-Maximilians-University, Feodor-Lynen-Str. 23, 81377 Munich (Germany); Högen, Tobias [Department of Neurology, Ludwig-Maximilians-University, Marchioninistr. 15, 81377 Munich (Germany); McLean, Pamela J. [Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224 (United States); Giese, Armin, E-mail: armin.giese@med.uni-muenchen.de [Center for Neuropathology and Prion Research, Ludwig-Maximilians-University, Feodor-Lynen-Str. 23, 81377 Munich (Germany)

    2016-08-12

    Synucleinopathies such as dementia with Lewy bodies or Parkinson’s disease are characterized by intracellular deposition of pathologically aggregated α-synuclein. The details of the molecular pathogenesis of PD and especially the conditions that lead to intracellular aggregation of α-synuclein and the role of these aggregates in cell death remain unknown. In cell free in vitro systems considerable knowledge about the aggregation processes has been gathered. In comparison, the knowledge about these aggregation processes in cells is far behind. In cells α-synuclein aggregates can be toxic. However, the crucial particle species responsible for decisive steps in pathogenesis such as seeding a continuing aggregation process and triggering cell death remain to be identified. In order to understand the complex nature of intracellular α-synuclein aggregate formation, we analyzed fluorescent particles formed by venus and α-synuclein-venus fusion proteins and α-synuclein-hemi-venus fusion proteins derived from gently lyzed cells. With these techniques we were able to identify and characterize α-synuclein oligomers formed in cells. Especially the use of α-synuclein-hemi-venus fusion proteins enabled us to identify very small α-synuclein oligomers with high sensitivity. Furthermore, we were able to study the molecular effect of heat shock protein 70, which is known to inhibit α-synuclein aggregation in cells. Heat shock protein 70 does not only influence the size of α-synuclein oligomers, but also their quantity. In summary, this approach based on fluorescence single particle spectroscopy, that is suited for high throughput measurements, can be used to detect and characterize intracellularly formed α-synuclein aggregates and characterize the effect of molecules that interfere with α-synuclein aggregate formation. - Highlights: • Single particle spectroscopy detects intracellular formed α-synuclein aggregates. • Fusion proteins allow detection of protein

  14. Sensitivity of the Single Particle Soot Photometer to different black carbon types

    Science.gov (United States)

    Laborde, M.; Mertes, P.; Zieger, P.; Dommen, J.; Baltensperger, U.; Gysel, M.

    2012-05-01

    Black carbon (BC) is now mainly of anthropogenic origin. It is the dominant light absorbing component of atmospheric aerosols, playing an important role in the earth's radiative balance and therefore relevant to climate change studies. In addition, BC is known to be harmful to human beings making it relevant to policy makers. Nevertheless, the measurement of BC remains biased by the instrument-based definition of BC. The Single Particle Soot Photometer (SP2), allows the measurement of the refractory BC (rBC) mass of individual particles using laser-induced incandescence. However, the SP2 needs an empirical calibration to retrieve the rBC mass from the incandescence signal and the sensitivity of the SP2 differs between different BC types. Ideally, for atmospheric studies, the SP2 should be calibrated using ambient particles containing a known mass of ambient rBC. However, such "ambient BC" calibration particles cannot easily be obtained and thus commercially available BC particles are commonly used for SP2 calibration instead. In this study we tested the sensitivity of the SP2 to different BC types in order to characterize the potential error introduced by using non-ambient BC for calibration. The sensitivity of the SP2 was determined, using an aerosol particle mass analyzer, for rBC from thermodenuded diesel exhaust, wood burning exhaust and ambient particles as well as for commercially available products: Aquadag® and fullerene soot. Thermodenuded, fresh diesel exhaust has been found to be ideal for SP2 calibration for two reasons. First, the small amount of non-BC matter upon emission reduces the risk of bias due to incomplete removal of non-BC matter and second, it is considered to represent atmospheric rBC in urban locations where diesel exhaust is the main source of BC. The SP2 was found to be up to 16% less sensitive to rBC from thermodenuded ambient particles (≤15 fg) than rBC from diesel exhaust, however, at least part of this difference can be explained

  15. Developing new optical imaging techniques for single particle and molecule tracking in live cells

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Wei [Iowa State Univ., Ames, IA (United States)

    2010-01-01

    Differential interference contrast (DIC) microscopy is a far-field as well as wide-field optical imaging technique. Since it is non-invasive and requires no sample staining, DIC microscopy is suitable for tracking the motion of target molecules in live cells without interfering their functions. In addition, high numerical aperture objectives and condensers can be used in DIC microscopy. The depth of focus of DIC is shallow, which gives DIC much better optical sectioning ability than those of phase contrast and dark field microscopies. In this work, DIC was utilized to study dynamic biological processes including endocytosis and intracellular transport in live cells. The suitability of DIC microscopy for single particle tracking in live cells was first demonstrated by using DIC to monitor the entire endocytosis process of one mesoporous silica nanoparticle (MSN) into a live mammalian cell. By taking advantage of the optical sectioning ability of DIC, we recorded the depth profile of the MSN during the endocytosis process. The shape change around the nanoparticle due to the formation of a vesicle was also captured. DIC microscopy was further modified that the sample can be illuminated and imaged at two wavelengths simultaneously. By using the new technique, noble metal nanoparticles with different shapes and sizes were selectively imaged. Among all the examined metal nanoparticles, gold nanoparticles in rod shapes were found to be especially useful. Due to their anisotropic optical properties, gold nanorods showed as diffraction-limited spots with disproportionate bright and dark parts that are strongly dependent on their orientation in the 3D space. Gold nanorods were developed as orientation nanoprobes and were successfully used to report the self-rotation of gliding microtubules on kinesin coated substrates. Gold nanorods were further used to study the rotational motions of cargoes during the endocytosis and intracellular transport processes in live mammalian

  16. Single particle analysis of TiO2in candy products using triple quadrupole ICP-MS.

    Science.gov (United States)

    Candás-Zapico, S; Kutscher, D J; Montes-Bayón, M; Bettmer, J

    2018-04-01

    Titanium dioxide (TiO 2 ) belongs to the materials that have gained great importance in many applications. In its particulate form (micro- or nanoparticles), it has entered a huge number of consumer products and food-grade TiO 2 , better known as E171 within the European Union, represents an important food additive. Thus, there is an increasing need for analytical methods able to detect and quantify such particles. In this regard, inductively coupled-mass spectrometry (ICP-MS), in particular single particle ICP-MS (spICP-MS), has gained importance due to its simplicity and ease of use. Nevertheless, the number of applications for Ti nanoparticles is rather limited. In this study, we have applied the spICP-MS strategy by comparing different measuring modes available in triple quadrupole ICP-MS. First, single quadrupole mode using the collision/reaction cell system was selected for monitoring the isotope 47 Ti. Different cell gases like He, O 2 and NH 3 were tested under optimised conditions for its applicability in spICP-MS of standard suspensions of TiO 2 . The determined analytical figures of merit were compared to those obtained by triple quadrupole mode using the 47 Ti or 48 Ti reaction products using O 2 and NH 3 as reaction gases. This comparison demonstrated that the triple quadrupole mode (TQ mode) was superior in terms of sensitivity due to the more efficient removal of spectral interferences. Particle size detection limits down to 26nm were obtained using the best instrumental conditions for TiO 2 particles at a dwell time of 10ms. Finally, the different measuring modes were applied to the analysis of chewing gum samples after a simple extraction procedure using an ultrasonic bath. The obtained results showed a good agreement for the detected particle size range using the different TQ modes. The size range of TiO 2 particles was determined to be between approximately 30 and 200nm, whereas roughly 40% of the particles were smaller than 100nm. For the

  17. High-Throughput Single-Particle Analysis of Metal-Enhanced Fluorescence in Free Solution Using Ag@SiO2Core-Shell Nanoparticles.

    Science.gov (United States)

    Yan, Ya; Meng, Lingyan; Zhang, Wenqiang; Zheng, Yan; Wang, Shuo; Ren, Bin; Yang, Zhilin; Yan, Xiaomei

    2017-09-22

    Metal-enhanced fluorescence (MEF) based on localized surface plasmon resonance (LSPR) is an effective strategy to increase the detection sensitivity in biotechnology and biomedicine. Because plasmonic nanoparticles are intrinsically heterogeneous, high-throughput single-particle analysis of MEF in free solution are highly demanded for the mechanistic understanding and control of this nanoscale process. Here, we report the application of a laboratory-built high-sensitivity flow cytometer (HSFCM) to investigate the fluorescence-enhancing effect of individual plasmonic nanoparticles on nearby fluorophore molecules. Ag@SiO 2 core-shell nanoparticles were used as the model system which comprised a silver core, a silica shell, and an FITC-doped thin layer of silica shell. FITC-doped silica nanoparticles of the same particle size but without silver core were used as the counterparts. Both the side scattering and fluorescence signals of single nanoparticles in suspension were measured simultaneously by the HSFCM at a speed of thousands of particles per minute. The roles of silver core size (40-100 nm) and fluorophore-metal distance (5-30 nm) were systematically examined. Fluorescence enhancement factor exceeding 30 was observed at silver core size of 70 nm and silica shell thickness of 5 nm. Compared with ensemble-averaged spectrofluorometric measurements, our experimental observation at the single-particle level was well supported by the finite difference time domain (FDTD) calculation. It allows us to achieve a fundamental understanding of MEF, which is important to the design and control of plasmonic nanostructures for efficient fluorescence enhancement.

  18. On the generalization of constraint programming and boolean satisfiability solving techniques to schedule a resource-constrained project consisting of multi-mode jobs

    Directory of Open Access Journals (Sweden)

    Alexander Schnell

    Full Text Available In our paper, we analyze new exact approaches for the multi-mode resource-constrained project scheduling (MRCPSP problem with the aim of makespan minimization. For the single-mode RCPSP (SRCPSP recent exact algorithms combine a Branch and Bound algorithm with principles from Constraint Programming (CP and Boolean Satisfiability Solving (SAT. We extend the above principles for the solution of MRCPSP instances. This generalization is on the one hand achieved on the modeling level. We propose three CP-based formulations of the MRCPSP for the G12 CP platform and the optimization framework SCIP which both provide solution techniques combining CP and SAT principles. For one of the latter we implemented a new global constraint for SCIP, which generalizes the domain propagation and explanation generation principles for renewable resources in the context of multi-mode jobs. Our constraint applies the above principles in a more general way than the existing global constraint in SCIP. We compare our approaches with the state-of-the-art exact algorithm from the literature on MRCPSP instances with 20 and 30 jobs. Our computational experiments show that we can outperform the latter approach on these instances. Furthermore, we are the first to close (find the optimal solution and prove its optimality for 628 open instances with 50 and 100 jobs from the literature. In addition, we improve the best known lower bound of 2815 instances and the best known upper bound of 151 instances. Keywords: Multi-mode resource-constrained project scheduling, Constraint programming, SAT solving, SCIP, Lazy clause generation, Exact algorithm

  19. Novel algorithm and MATLAB-based program for automated power law analysis of single particle, time-dependent mean-square displacement

    Science.gov (United States)

    Umansky, Moti; Weihs, Daphne

    2012-08-01

    In many physical and biophysical studies, single-particle tracking is utilized to reveal interactions, diffusion coefficients, active modes of driving motion, dynamic local structure, micromechanics, and microrheology. The basic analysis applied to those data is to determine the time-dependent mean-square displacement (MSD) of particle trajectories and perform time- and ensemble-averaging of similar motions. The motion of particles typically exhibits time-dependent power-law scaling, and only trajectories with qualitatively and quantitatively comparable MSD should be ensembled. Ensemble averaging trajectories that arise from different mechanisms, e.g., actively driven and diffusive, is incorrect and can result inaccurate correlations between structure, mechanics, and activity. We have developed an algorithm to automatically and accurately determine power-law scaling of experimentally measured single-particle MSD. Trajectories can then categorized and grouped according to user defined cutoffs of time, amplitudes, scaling exponent values, or combinations. Power-law fits are then provided for each trajectory alongside categorized groups of trajectories, histograms of power laws, and the ensemble-averaged MSD of each group. The codes are designed to be easily incorporated into existing user codes. We expect that this algorithm and program will be invaluable to anyone performing single-particle tracking, be it in physical or biophysical systems. Catalogue identifier: AEMD_v1_0 Program summary URL:http://cpc.cs.qub.ac.uk/summaries/AEMD_v1_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: Standard CPC licence, http://cpc.cs.qub.ac.uk/licence/licence.html No. of lines in distributed program, including test data, etc.: 25 892 No. of bytes in distributed program, including test data, etc.: 5 572 780 Distribution format: tar.gz Programming language: MATLAB (MathWorks Inc.) version 7.11 (2010b) or higher, program

  20. Evaluation of number concentration quantification by single-particle inductively coupled plasma mass spectrometry: microsecond vs. millisecond dwell times.

    Science.gov (United States)

    Abad-Álvaro, Isabel; Peña-Vázquez, Elena; Bolea, Eduardo; Bermejo-Barrera, Pilar; Castillo, Juan R; Laborda, Francisco

    2016-07-01

    The quality of the quantitative information in single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS) depends directly on the number concentration of the nanoparticles in the sample analyzed, which is proportional to the flux of nanoparticles through the plasma. Particle number concentrations must be selected in accordance with the data acquisition frequency, to control the precision from counting statistics and the bias, which is produced by the occurrence of multiple-particle events recorded as single-particle events. With quadrupole mass spectrometers, the frequency of data acquisition is directly controlled by the dwell time. The effect of dwell times from milli- to microseconds (10 ms, 5 ms, 100 μs, and 50 μs) on the quality of the quantitative data has been studied. Working with dwell times in the millisecond range, precision figures about 5 % were achieved, whereas using microsecond dwell times, the suitable fluxes of nanoparticles are higher and precision was reduced down to 1 %; this was independent of the dwell time selected. Moreover, due to the lower occurrence of multiple-nanoparticle events, linear ranges are wider when dwell times equal to or shorter than 100 μs are used. A calculation tool is provided to determine the optimal concentration for any instrument or experimental conditions selected. On the other hand, the use of dwell times in the microsecond range reduces significantly the contribution of the background and/or the presence of dissolved species, in comparison with the use of millisecond dwell times. Although the use of dwell times equal to or shorter than 100 μs offers improved performance working in single-particle mode, the use of conventional dwell times (3-10 ms) should not be discarded, once their limitations are known.

  1. Investigation of the single Particle Structure of the neutron-rich Sodium Isotopes $^{27-31}\\!$Na

    CERN Multimedia

    2002-01-01

    We propose to study the single particle structure of the neutron-rich isotopes $^{27-31}\\!$Na. These isotopes will be investigated via neutron pickup reactions in inverse kinematics on a deuterium and a beryllium target. Scattered beam particles and transfer products are detected in a position sensitive detector located around 0$^\\circ$. De-excitation $\\gamma$-rays emitted after an excited state has been populated will be registered by the MINIBALL Germanium array. The results will shed new light on the structure of the neutron-rich sodium isotopes and especially on the region of strong deformation around the N=20 nucleus $^{31}\\!$Na.

  2. Characterization of silver nanoparticle aggregates using single particle-inductively coupled plasma-mass spectrometry (spICP-MS).

    Science.gov (United States)

    Kim, Hyun-A; Lee, Byung-Tae; Na, So-Young; Kim, Kyoung-Woong; Ranville, James F; Kim, Soon-Oh; Jo, Eunhye; Eom, Ig-Chun

    2017-03-01

    The single particle-inductively coupled plasma-mass spectrometry was applied to characterize the aggregates of AgNPs. was applied to characterize the aggregates of AgNPs. Two sizes of citrate-AgNPs and PVP-AgNPs were used at relatively high and predicted environmental concentrations under various ionic strengths. Citrate-AgNP aggregated with increases in the ionic strength, whereas PVP-AgNPs were sterically stable. The critical coagulation concentrations were 85 mM and 100 mM NaNO 3 for 60 nm and 100 nm citrate-AgNPs at 2 mg L -1 as total Ag obtained by dynamic light scattering (DLS). At 2 mg L -1 as total Ag, the mass of an aggregate gradually increased with increasing ionic strength for both citrate-AgNP during spICP-MS analyses. The average number of single particles derived from the mass in an aggregate was calculated to be 8.68 and 5.95 for 60 nm and 100 nm citrate-AgNPs at 85 mM and 100 mM NaNO 3 , respectively after 2 h. The mass fractal dimensions were determined to be 2.97 and 2.83, further implying that the aggregate structures were very rigid and compact. Only marginal increases in the average mass and number of single particles in the aggregate units were found during 24 h under environmentally relevant AgNP concentrations. The average number of single particles constituting an aggregate unit for 60 nm and 100 nm citrate-AgNPs was 1.24 and 1.37 after 24 h at a high ionic strength. These results indicate that under environmentally relevant conditions, the collision frequency is predominant in the aggregation and that NPs are likely to encounter natural colloids such as clay and organic matter to form hetero-aggregates. Copyright © 2016. Published by Elsevier Ltd.

  3. Pre-asymptotic behavior of single-particle overlap integrals of non-Borromean two-neutron halos

    International Nuclear Information System (INIS)

    Timofeyuk, N.K.; Tostevin, J.A.; Blokhintsev, L.D.

    2003-01-01

    For non-Borromean two-neutron halo nuclei, modifications to the behavior of single-particle overlap integrals will arise due to the correlations of the two interacting nucleons in the halo. An additional contribution to the overlap integral can be obtained using the Feynman diagram approach. This additional term is modeled using a simple local potential model. We show that these modifications may play a role in detailed interpretations of experimental results from single-nucleon knockout, transfer, and other reactions that probe the single-nucleon overlap functions

  4. Single particle analysis of thylakoid proteins from Thermosynechococcus elongatus and Synechocystis 6803 : Localization of the CupA subunit of NDH-1

    NARCIS (Netherlands)

    Folea, I. Mihaela; Zhang, Pengpeng; Nowaczyk, Marc M.; Ogawa, Teruo; Aro, Eva-Marl; Boekema, Egbert J.; Aro, Eva-Mari

    The larger protein complexes of the cyanobacterial photosynthetic membrane of Thermosynechoccus elongatus and Synechocystis 6803 were studied by single particle electron microscopy after detergent solubilization, without any purification steps. Besides the "standard" L-shaped NDH-1L complex, related

  5. A comparison of single particle tracking and temporal image correlation spectroscopy for quantitative analysis of endosome motility

    DEFF Research Database (Denmark)

    Lund, F. W.; Wustner, D.

    2013-01-01

    Single particle tracking (SPT) is becoming a standard method to extract transport parameters from time-lapse image sequences of fluorescent vesicles in living cells. Another method to obtain these data is temporal image correlation spectroscopy (TICS), but this method is less often used for measu......Single particle tracking (SPT) is becoming a standard method to extract transport parameters from time-lapse image sequences of fluorescent vesicles in living cells. Another method to obtain these data is temporal image correlation spectroscopy (TICS), but this method is less often used...... for measurement of intracellular vesicle transport. Here, we present an extensive comparison of SPT and TICS. First we examine the effect of photobleaching, shading and noise on SPT and TICS analysis using simulated image sequences. To this end, we developed a simple photophysical model, which relates spatially...... varying illumination intensity to the bleaching propensity and fluorescence intensity of the moving particles. We found that neither SPT nor TICS are affected by photobleaching per se, but the transport parameters obtained by both methods are sensitive to the signal-to-noise ratio. In addition, the number...

  6. Detection of lead nanoparticles in game meat by single particle ICP-MS following use of lead-containing bullets.

    Science.gov (United States)

    Kollander, Barbro; Widemo, Fredrik; Ågren, Erik; Larsen, Erik H; Loeschner, Katrin

    2017-03-01

    This study investigated whether game meat may contain nanoparticles of lead from ammunition. Lead nanoparticles in the range 40 to 750 nm were detected by ICP-MS in single particle mode in game shot with lead-containing bullets. The median diameter of the detected nanoparticles was around 60 nm. The particle mass concentration ranged from 290 to 340 ng/g meat and the particle number concentrations from 27 to 50 million particles/g meat. The size limit of detection strongly depended on the level of dissolved lead and was in the range of 40 to 80 nm. In game meat sampled more than 10 cm away from the wound channel, no lead particles with a diameter larger than 40 nm were detected. In addition to dissolved lead in meat that originated from particulates, the presence of lead nano particles in game meat represents a hitherto unattended source of lead with a largely unknown toxicological impact to humans. Graphical Abstract Detection of lead nanoparticles in game meat by single particle ICP-MS following use of leadcontaining bullets.

  7. Reconstruction from limited single-particle diffraction data via simultaneous determination of state, orientation, intensity, and phase.

    Science.gov (United States)

    Donatelli, Jeffrey J; Sethian, James A; Zwart, Peter H

    2017-07-11

    Free-electron lasers now have the ability to collect X-ray diffraction patterns from individual molecules; however, each sample is delivered at unknown orientation and may be in one of several conformational states, each with a different molecular structure. Hit rates are often low, typically around 0.1%, limiting the number of useful images that can be collected. Determining accurate structural information requires classifying and orienting each image, accurately assembling them into a 3D diffraction intensity function, and determining missing phase information. Additionally, single particles typically scatter very few photons, leading to high image noise levels. We develop a multitiered iterative phasing algorithm to reconstruct structural information from single-particle diffraction data by simultaneously determining the states, orientations, intensities, phases, and underlying structure in a single iterative procedure. We leverage real-space constraints on the structure to help guide optimization and reconstruct underlying structure from very few images with excellent global convergence properties. We show that this approach can determine structural resolution beyond what is suggested by standard Shannon sampling arguments for ideal images and is also robust to noise.

  8. Source characterization of urban particles from meat smoking activities in Chongqing, China using single particle aerosol mass spectrometry.

    Science.gov (United States)

    Chen, Yang; Wenger, John C; Yang, Fumo; Cao, Junji; Huang, Rujin; Shi, Guangming; Zhang, Shumin; Tian, Mi; Wang, Huanbo

    2017-09-01

    A Single Particle Aerosol Mass Spectrometer (SPAMS) was deployed in the urban area of Chongqing to characterize the particles present during a severe particulate pollution event that occurred in winter 2014-2015. The measurements were made at a time when residents engaged in traditional outdoor meat smoking activities to preserve meat before the Chinese Spring Festival. The measurement period was predominantly characterized by stagnant weather conditions, highly elevated levels of PM 2.5 , and low visibility. Eleven major single particle types were identified, with over 92.5% of the particles attributed to biomass burning emissions. Most of the particle types showed appreciable signs of aging in the stagnant air conditions. To simulate the meat smoking activities, a series of controlled smoldering experiments was conducted using freshly cut pine and cypress branches, both with and without wood logs. SPAMS data obtained from these experiments revealed a number of biomass burning particle types, including an elemental and organic carbon (ECOC) type that proved to be the most suitable marker for meat smoking activities. The traditional activity of making preserved meat in southwestern China is shown here to be a major source of particulate pollution. Improved measures to reduce emissions from the smoking of meat should be introduced to improve air quality in regions where smoking meat activity prevails. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. Site-Specific SERS Assay for Survivin Protein Dimer: From Ensemble Experiments to Correlative Single-Particle Imaging.

    Science.gov (United States)

    Wissler, Jörg; Bäcker, Sandra; Feis, Alessandro; Knauer, Shirley K; Schlücker, Sebastian

    2017-08-01

    An assay for Survivin, a small dimeric protein which functions as modulator of apoptosis and cell division and serves as a promising diagnostic biomarker for different types of cancer, is presented. The assay is based on switching on surface-enhanced Raman scattering (SERS) upon incubation of the Survivin protein dimer with Raman reporter-labeled gold nanoparticles (AuNP). Site-specificity is achieved by complexation of nickel-chelated N-nitrilo-triacetic acid (Ni-NTA) anchors on the particle surface by multiple histidines (His 6 -tag) attached to each C-terminus of the centrosymmetric protein dimer. Correlative single-particle analysis using light sheet laser microscopy enables the simultaneous observation of both elastic and inelastic light scattering from the same sample volume. Thereby, the SERS-inactive AuNP-protein monomers can be directly discriminated from the SERS-active AuNP-protein dimers/oligomers. This information, i.e. the percentage of SERS-active AuNP in colloidal suspension, is not accessible from conventional SERS experiments due to ensemble averaging. The presented correlative single-particle approach paves the way for quantitative site-specific SERS assays in which site-specific protein recognition by small chemical and in particular supramolecular ligands can be tested. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Single particle tracking of internalized metallic nanoparticles reveals heterogeneous directed motion after clathrin dependent endocytosis in mouse chromaffin cells

    Science.gov (United States)

    Gabriel, Manuela; Moya-Díaz, José; Gallo, Luciana I.; Marengo, Fernando D.; Estrada, Laura C.

    2018-01-01

    Most accepted single particle tracking methods are able to obtain high-resolution trajectories for relatively short periods of time. In this work we apply a straightforward combination of single-particle tracking microscopy and metallic nanoparticles internalization on mouse chromaffin cells to unveil the intracellular trafficking mechanism of metallic-nanoparticle-loaded vesicles (MNP-V) complexes after clathrin dependent endocytosis. We found that directed transport is the major route of MNP-Vs intracellular trafficking after stimulation (92.6% of the trajectories measured). We then studied the MNP-V speed at each point along the trajectory, and found that the application of a second depolarization stimulus during the tracking provokes an increase in the percentage of low-speed trajectory points in parallel with a decrease in the number of high-speed trajectory points. This result suggests that stimulation may facilitate the compartmentalization of internalized MNPs in a more restricted location such as was already demonstrated in neuronal and neuroendocrine cells (Bronfman et al 2003 J. Neurosci. 23 3209-20). Although further experiments will be required to address the mechanisms underlying this transport dynamics, our studies provide quantitative evidence of the heterogeneous behavior of vesicles mobility after endocytosis in chromaffin cells highlighting the potential of MNPs as alternative labels in optical microscopy to provide new insights into the vesicles dynamics in a wide variety of cellular environments.

  11. Enhancement of single particle rare earth doped NaYF4: Yb, Er emission with a gold shell

    International Nuclear Information System (INIS)

    Li, Ling; Green, Kory; Hallen, Hans; Lim, Shuang Fang

    2015-01-01

    Upconversion of infrared light to visible light has important implications for bioimaging. However, the small absorption cross-section of rare earth dopants has limited the efficiency of these anti-Stokes nanomaterials. We present enhanced excitation absorption and single particle fluorescent emission of sodium yttrium fluoride, NaYF 4 : Yb, Er based upconverting nanoparticles coated with a gold nanoshell through surface plasmon resonance. The single gold-shell coated nanoparticles show enhanced absorption in the near infrared, enhanced total emission intensity, and increased green relative to red emission. We also show differences in enhancement between single and aggregated gold shell nanoparticles. The surface plasmon resonance of the gold-shell coated nanoparticle is shown to be dependent on the shell thickness. In contrast to other reported results, our single particle experimental observations are corroborated by finite element calculations that show where the green/red emission enhancement occurs, and what portion of the enhancement is due to electromagnetic effects. We find that the excitation enhancement and green/red emission ratio enhancement occurs at the corners and edges of the doped emissive core. (paper)

  12. Characteristic rotational behaviors of rod-shaped cargo revealed by automated five-dimensional single particle tracking.

    Science.gov (United States)

    Chen, Kuangcai; Gu, Yan; Sun, Wei; Bin Dong; Wang, Gufeng; Fan, Xinxin; Xia, Tian; Fang, Ning

    2017-10-12

    We report an automated single particle tracking technique for tracking the x, y, z coordinates, azimuthal and elevation angles of anisotropic plasmonic gold nanorod probes in live cells. These five spatial coordinates are collectively referred to as 5D. This method overcomes a long-standing challenge in distinguishing rotational motions from translational motions in the z-axis in differential interference contrast microscopy to result in full disclosure of nanoscale motions with high accuracy. Transferrin-coated endocytic gold nanorod cargoes initially undergo active rotational diffusion and display characteristic rotational motions on the membrane. Then as the cargoes being enclosed in clathrin-coated pits, they slow down the active rotation and experience a quiet period before they restore active rotational diffusion after fission and eventually being transported away from the original entry spots. Finally, the 3D trajectories and the accompanying rotational motions of the cargoes are resolved accurately to render the intracellular transport process in live cells.Distinguishing rotational motions from translational motions in the z-axis has been a long-standing challenge. Here the authors develop a five-dimensional single particle tracking method to detect rotational behaviors of nanocargos during clathrin-mediated endocytosis and intracellular transport.

  13. Interplay between Single-Particle and Collective Effects in the Odd-A Cu Isotopes beyond N=40

    CERN Document Server

    Stefanescu, I; Balabanski, D L; Blasi, N; Blazhev, A; Bree, N; Cederkäll, J; Cocolios, T E; Davinson, T; Diriken, J; Eberth, J; Ekström, A; Fedorov, D; Fedosseev, V; Fraile-Prieto, L M; Franchoo, S; Gladnishki, K; Huyse, M; Ivanov, O; Ivanov, I; Iwanicki, J; Jolie, J; Konstantinopoulos, T; Kröll, Th; Krücken, R; Köster, U; Lagoyannis, A; Lo Bianco, G; Maierbeck, P; Marsh, B A; Napiorkowski, P; Patronis, N; Pauwels, D; Rainovski, G; Reiter, P; Riisager, K; Seliverstov, M; Sletten, G; Van de Walle, J; Van Duppen, P; Voulot, D; Warr, N; Wenander, F; Wrzosek, K

    2008-01-01

    Collective properties of the low-lying levels in the odd-A 67–73Cu were investigated by Coulomb excitation with radioactive beams. The beams were produced at ISOLDE and postaccelerated by REX-ISOLDE up to 2.99  MeV/u. In 67,69Cu, low-lying 1/2-, 5/2-, and 7/2- states were populated. In 71,73Cu, besides the known transitions deexciting the single-particle-like 5/2- and core-coupled 7/2- levels, γ rays of 454 and 135 keV, respectively, were observed for the first time. Based on a reanalysis of β-decay work and comparison with the systematics, a spin 1/2- is suggested for these excited states. Three B(E2) values were determined in each of the four isotopes. The results indicate a significant change in the structure of the odd-A Cu isotopes beyond N=40 where single-particle-like and collective levels are suggested to coexist at very low excitation energies.

  14. Considerations of particle vaporization and analyte diffusion in single-particle inductively coupled plasma-mass spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Ho, Koon-Sing; Lui, Kwok-On; Lee, Kin-Ho; Chan, Wing-Tat, E-mail: wtchan@hku.hk

    2013-11-01

    The intensity of individual gold nanoparticles with nominal diameters of 80, 100, 150, and 200 nm was measured using single-particle inductively coupled plasma-mass spectrometry (ICP-MS). Since the particles are not perfectly monodisperse, a distribution of ICP-MS intensity was obtained for each nominal diameter. The distribution of particle mass was determined from the transmission electron microscopy (TEM) image of the particles. The distribution of ICP-MS intensity and the distribution of particle mass for each nominal diameter were correlated to give a calibration curve. The calibration curves are linear, but the slope decreases as the nominal diameter increases. The reduced slope is probably due to a smaller degree of vaporization of the large particles. In addition to the degree of particle vaporization, the rate of analyte diffusion in the ICP is an important factor that determines the measured ICP-MS intensity. Simulated ICP-MS intensity versus particle size was calculated using a simple computer program that accounts for the vaporization rate of the gold nanoparticles and the diffusion rate and degree of ionization of the gold atoms. The curvature of the simulated calibration curves changes with sampling depth because the effects of particle vaporization and analyte diffusion on the ICP-MS intensity are dependent on the residence time of the particle in the ICP. Calibration curves of four hypothetical particles representing the four combinations of high and low boiling points (2000 and 4000 K) and high and low analyte diffusion rates (atomic masses of 10 and 200 Da) were calculated to further illustrate the relative effects of particle vaporization and analyte diffusion. The simulated calibration curves show that the sensitivity of single-particle ICP-MS is smaller than that of the ICP-MS measurement of continuous flow of standard solutions by a factor of 2 or more. Calibration using continuous flow of standard solution is semi-quantitative at best. An

  15. Quantitative single-particle digital autoradiography with α-particle emitters for targeted radionuclide therapy using the iQID camera

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Brian W., E-mail: brian.miller@pnnl.gov [Pacific Northwest National Laboratory, Richland, Washington 99354 and College of Optical Sciences, The University of Arizona, Tucson, Arizona 85719 (United States); Frost, Sofia H. L.; Frayo, Shani L.; Kenoyer, Aimee L.; Santos, Erlinda; Jones, Jon C.; Orozco, Johnnie J. [Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 (United States); Green, Damian J.; Press, Oliver W.; Pagel, John M.; Sandmaier, Brenda M. [Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 and Department of Medicine, University of Washington, Seattle, Washington 98195 (United States); Hamlin, Donald K.; Wilbur, D. Scott [Department of Radiation Oncology, University of Washington, Seattle, Washington 98195 (United States); Fisher, Darrell R. [Dade Moeller Health Group, Richland, Washington 99354 (United States)

    2015-07-15

    Purpose: Alpha-emitting radionuclides exhibit a potential advantage for cancer treatments because they release large amounts of ionizing energy over a few cell diameters (50–80 μm), causing localized, irreparable double-strand DNA breaks that lead to cell death. Radioimmunotherapy (RIT) approaches using monoclonal antibodies labeled with α emitters may thus inactivate targeted cells with minimal radiation damage to surrounding tissues. Tools are needed to visualize and quantify the radioactivity distribution and absorbed doses to targeted and nontargeted cells for accurate dosimetry of all treatment regimens utilizing α particles, including RIT and others (e.g., Ra-223), especially for organs and tumors with heterogeneous radionuclide distributions. The aim of this study was to evaluate and characterize a novel single-particle digital autoradiography imager, the ionizing-radiation quantum imaging detector (iQID) camera, for use in α-RIT experiments. Methods: The iQID camera is a scintillator-based radiation detection system that images and identifies charged-particle and gamma-ray/x-ray emissions spatially and temporally on an event-by-event basis. It employs CCD-CMOS cameras and high-performance computing hardware for real-time imaging and activity quantification of tissue sections, approaching cellular resolutions. In this work, the authors evaluated its characteristics for α-particle imaging, including measurements of intrinsic detector spatial resolutions and background count rates at various detector configurations and quantification of activity distributions. The technique was assessed for quantitative imaging of astatine-211 ({sup 211}At) activity distributions in cryosections of murine and canine tissue samples. Results: The highest spatial resolution was measured at ∼20 μm full width at half maximum and the α-particle background was measured at a rate as low as (2.6 ± 0.5) × 10{sup −4} cpm/cm{sup 2} (40 mm diameter detector area

  16. Quantitative single-particle digital autoradiography with α-particle emitters for targeted radionuclide therapy using the iQID camera.

    Science.gov (United States)

    Miller, Brian W; Frost, Sofia H L; Frayo, Shani L; Kenoyer, Aimee L; Santos, Erlinda; Jones, Jon C; Green, Damian J; Hamlin, Donald K; Wilbur, D Scott; Fisher, Darrell R; Orozco, Johnnie J; Press, Oliver W; Pagel, John M; Sandmaier, Brenda M

    2015-07-01

    Alpha-emitting radionuclides exhibit a potential advantage for cancer treatments because they release large amounts of ionizing energy over a few cell diameters (50-80 μm), causing localized, irreparable double-strand DNA breaks that lead to cell death. Radioimmunotherapy (RIT) approaches using monoclonal antibodies labeled with α emitters may thus inactivate targeted cells with minimal radiation damage to surrounding tissues. Tools are needed to visualize and quantify the radioactivity distribution and absorbed doses to targeted and nontargeted cells for accurate dosimetry of all treatment regimens utilizing α particles, including RIT and others (e.g., Ra-223), especially for organs and tumors with heterogeneous radionuclide distributions. The aim of this study was to evaluate and characterize a novel single-particle digital autoradiography imager, the ionizing-radiation quantum imaging detector (iQID) camera, for use in α-RIT experiments. The iQID camera is a scintillator-based radiation detection system that images and identifies charged-particle and gamma-ray/x-ray emissions spatially and temporally on an event-by-event basis. It employs CCD-CMOS cameras and high-performance computing hardware for real-time imaging and activity quantification of tissue sections, approaching cellular resolutions. In this work, the authors evaluated its characteristics for α-particle imaging, including measurements of intrinsic detector spatial resolutions and background count rates at various detector configurations and quantification of activity distributions. The technique was assessed for quantitative imaging of astatine-211 ((211)At) activity distributions in cryosections of murine and canine tissue samples. The highest spatial resolution was measured at ∼20 μm full width at half maximum and the α-particle background was measured at a rate as low as (2.6 ± 0.5) × 10(-4) cpm/cm(2) (40 mm diameter detector area). Simultaneous imaging of multiple tissue sections was

  17. Quantitative determination of carbonaceous particle mixing state in Paris using single-particle mass spectrometer and aerosol mass spectrometer measurements

    Directory of Open Access Journals (Sweden)

    R. M. Healy

    2013-09-01

    Full Text Available Single-particle mixing state information can be a powerful tool for assessing the relative impact of local and regional sources of ambient particulate matter in urban environments. However, quantitative mixing state data are challenging to obtain using single-particle mass spectrometers. In this study, the quantitative chemical composition of carbonaceous single particles has been determined using an aerosol time-of-flight mass spectrometer (ATOFMS as part of the MEGAPOLI 2010 winter campaign in Paris, France. Relative peak areas of marker ions for elemental carbon (EC, organic aerosol (OA, ammonium, nitrate, sulfate and potassium were compared with concurrent measurements from an Aerodyne high-resolution time-of-flight aerosol mass spectrometer (HR-ToF-AMS, a thermal–optical OCEC analyser and a particle into liquid sampler coupled with ion chromatography (PILS-IC. ATOFMS-derived estimated mass concentrations reproduced the variability of these species well (R2 = 0.67–0.78, and 10 discrete mixing states for carbonaceous particles were identified and quantified. The chemical mixing state of HR-ToF-AMS organic aerosol factors, resolved using positive matrix factorisation, was also investigated through comparison with the ATOFMS dataset. The results indicate that hydrocarbon-like OA (HOA detected in Paris is associated with two EC-rich mixing states which differ in their relative sulfate content, while fresh biomass burning OA (BBOA is associated with two mixing states which differ significantly in their OA / EC ratios. Aged biomass burning OA (OOA2-BBOA was found to be significantly internally mixed with nitrate, while secondary, oxidised OA (OOA was associated with five particle mixing states, each exhibiting different relative secondary inorganic ion content. Externally mixed secondary organic aerosol was not observed. These findings demonstrate the range of primary and secondary organic aerosol mixing states in Paris. Examination of the

  18. Single particle and molecular assembly analysis of polyribosomes by single- and double-tilt cryo electron tomography

    Energy Technology Data Exchange (ETDEWEB)

    Myasnikov, Alexander G. [IGBMC (Institute of Genetics and of Molecular and Cellular Biology), Department of Integrative Structural Biology, Centre National de la Recherche Scientifique (CNRS) UMR 7104/ Institut National de la Santé de la Recherche Médicale INSERM U964/ Université de Strasbourg, 1 rue Laurent Fries, 67404 Illkirch (France); Afonina, Zhanna A. [Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region (Russian Federation); Klaholz, Bruno P., E-mail: klaholz@igbmc.fr [IGBMC (Institute of Genetics and of Molecular and Cellular Biology), Department of Integrative Structural Biology, Centre National de la Recherche Scientifique (CNRS) UMR 7104/ Institut National de la Santé de la Recherche Médicale INSERM U964/ Université de Strasbourg, 1 rue Laurent Fries, 67404 Illkirch (France)

    2013-03-15

    Cryo electron tomography (cryo-ET) can provide cellular and molecular structural information on various biological samples. However, the detailed interpretation of tomograms reconstructed from single-tilt data tends to suffer from low signal-to-noise ratio and artefacts caused by some systematically missing angular views. While these can be overcome by sub-tomogram averaging, they remain limiting for the analysis of unique structures. Double-tilt ET can improve the tomogram quality by acquiring a second tilt series after an in-plane rotation, but its usage is not widespread yet because it is considered technically demanding and it is rarely used under cryo conditions. Here we show that double-tilt cryo-ET improves the quality of 3D reconstructions so significantly that even single particle analysis can be envisaged despite of the intrinsically low image contrast obtained from frozen-hydrated specimens. This is illustrated by the analysis of eukaryotic polyribosomes in which individual ribosomes were reconstructed using single-tilt, partial and full double-tilt geometries. The improved tomograms favour the faster convergence of iterative sub-tomogram averaging and allow a better 3D classification using multivariate statistical analysis. Our study of single particles and molecular assemblies within polysomes illustrates that the dual-axis approach is particularly useful for cryo applications of ET, both for unique objects and for structures that can be classified and averaged. - Highlights: ► Double-tilt cryo-ET improves 3D reconstructions thus making single particle analysis possible. ► Dual-axis cryo-ET data favour a faster convergence of iterative sub-tomogram averaging. ► Individual ribosomes were reconstructed from single-tilt, partial/ full double-tilt geometries. ► Double-tilt cryo-ET facilitates analysis of larger molecular assemblies such as in cell sections. ► Dual-axis cryo-ET is applicable to unique objects and to structures that can be

  19. Strong reduction of spectral heterogeneity in gold bipyramids for single-particle and single-molecule plasmon sensing.

    Science.gov (United States)

    Peters, S M E; Verheijen, M A; Prins, M W J; Zijlstra, P

    2016-01-15

    Single metal nanoparticles are attractive biomolecular sensors. Binding of analyte to a functional particle results in a plasmon shift that can be conveniently monitored in a far-field optical microscope. Heterogeneities in spectral properties of individual particles in an ensemble affect the reliability of a single-particle plasmon sensor, especially when plasmon shifts are monitored in real-time using a fixed irradiation wavelength. We compare the spectral heterogeneity of different plasmon sensor geometries (gold nanospheres, nanorods, and bipyramids) and correlate this to their size and aspect-ratio dispersion. We show that gold bipyramids exhibit a strongly reduced heterogeneity in aspect ratio and plasmon wavelength compared to commonly used gold nanorods. We show that this translates into a significantly improved homogeneity of the response to molecular binding without compromising single-molecule sensitivity.

  20. Investigation on the biological effects of pharynx irradiation by single-particle microbeam and C.elegans immobilization

    International Nuclear Information System (INIS)

    Guo Xiaoying; Yang Gen; Chen Lianyun; Wu Lijun; Li Buqing

    2010-01-01

    Using C.elegans- with clear genetic background, easy genetic maneuverability, small individual, transparence, easily penetrated by a variety of particle as a in vivo model organism, irradiation damage at the individual level of the signal transduction and the mechanism research were investigated. In order to radiate accurately, the worms need Immobilize. The results showed that the ether: ethanol = 1:1 mixture, enabled the worms quickly anesthesia, and kept the worms Immobilization in the whole irradiation process, then quickly recovered after irradiation and recovery rate of 100%. On the basis, the laved and the apoptotic cells in the distal gonad would be test when the worm pharynx were irradiated by single-particle microbeam. The primary results showed that the apoptotic cells in distal gonad significantly increased when the worm pharynx were irradiated 5000 particles. (authors)

  1. Insight into the three-dimensional structure of maize chlorotic mottle virus revealed by Cryo-EM single particle analysis.

    Science.gov (United States)

    Wang, Chun-Yan; Zhang, Qin-Fen; Gao, Yuan-Zhu; Zhou, Xue-Ping; Ji, Gang; Huang, Xiao-Jun; Hong, Jian; Zhang, Chuan-Xi

    2015-11-01

    Maize chlorotic mottle virus (MCMV) is the only member of the Machlomovirus genus in the family Tombusviridae. Here, we obtained the Cryo-EM structure of MCMV by single particle analysis with most local resolution at approximately 4 Å. The Cα backbone was built based on residues with bulky side chains. The resolved C-terminus of the capsid protein subunit and obvious openings at the 2-fold axis demonstrated the compactness of the asymmetric unit, which indicates an important role in the stability of MCMV. The Asp116 residue from each subunit around the 5-fold and 3-fold axes contributed to the negative charges in the centers of the pentamers and hexamers, which might serve as a solid barrier against the leakage of genomic RNA. Finally, the loops most exposed on the surface were analyzed and are proposed to be potential functional sites related to MCMV transmission. Copyright © 2015 Elsevier Inc. All rights reserved.

  2. Elucidating distinct ion channel populations on the surface of hippocampal neurons via single-particle tracking recurrence analysis

    Science.gov (United States)

    Sikora, Grzegorz; Wyłomańska, Agnieszka; Gajda, Janusz; Solé, Laura; Akin, Elizabeth J.; Tamkun, Michael M.; Krapf, Diego

    2017-12-01

    Protein and lipid nanodomains are prevalent on the surface of mammalian cells. In particular, it has been recently recognized that ion channels assemble into surface nanoclusters in the soma of cultured neurons. However, the interactions of these molecules with surface nanodomains display a considerable degree of heterogeneity. Here, we investigate this heterogeneity and develop statistical tools based on the recurrence of individual trajectories to identify subpopulations within ion channels in the neuronal surface. We specifically study the dynamics of the K+ channel Kv1.4 and the Na+ channel Nav1.6 on the surface of cultured hippocampal neurons at the single-molecule level. We find that both these molecules are expressed in two different forms with distinct kinetics with regards to surface interactions, emphasizing the complex proteomic landscape of the neuronal surface. Further, the tools presented in this work provide new methods for the analysis of membrane nanodomains, transient confinement, and identification of populations within single-particle trajectories.

  3. Intra-Nuclear Single-Particle Tracking (I-SPT) to Reveal the Functional Architecture of Chromosomes.

    Science.gov (United States)

    Récamier, Vincent

    2016-01-01

    Chromosome architecture needs to be investigated in relation with the chemical function of DNA. The kinetics of gene expression, DNA replication, and repair are driven by the mechanisms by which a functional nuclear protein finds its substrate in the nucleus. Single-particle tracking (SPT) is a method to quantify fluorescent molecules dynamics from the tracks of the single molecules recorded by high-resolution microscopes. SPT offers direct observation of the movement and single-molecule resolution. Usually SPT is performed on membranes because of higher contrast. Here, we introduce a novel method to record the trajectories of weakly fluorescent molecules in the nucleus of living cells. I-SPT uses some specific detection and analysis tools to enable the computation of reliable statistics on nuclear particle movement.

  4. Second order single particle dynamics in quasi-isochronous storage rings and its application to the LNLS-UVX ring

    Energy Technology Data Exchange (ETDEWEB)

    Lin Liu (Pro-Reitoria de Pesquisa, Univ. Estadual de Campinas, SP (Brazil) Lab. Nacional de Luz Sincrotron-LNLS, Campinas, SP (Brazil)); Concalves da Silva, C.E.T. (Inst. de Fisica Gleb Wataghin, Univ. Estadual de Campinas, SP (Brazil) Lab. Nacional de Luz Sincrotron-LNLS, Campinas, SP (Brazil))

    1993-05-15

    We analyze the second order single particle longitudinal dynamics in a quasi-isochronous storage ring. We expand the momentum compaction factor to include the effects of second order terms taking sextupoles into account and of transverse betatron oscillations. The introduction of nonlinearities due to higher order terms results in a second stability region for longitudinal phase oscillations, in addition to the well known linear stable operation point. The conditions for this new solution to fall within the energy acceptance of the storage ring are presented. Inclusion of transverse motion coupling may lead to either a reduction or an enhancement of the stable longitudinal phase-space regions. The analysis is applied to the LNLS 1.15 GeV UVX electron storage ring, indicating that it should be possible to operate this ring in a quasi-isochronous mode. (orig.).

  5. Stereoscopy of dust density waves under microgravity: Velocity distributions and phase-resolved single-particle analysis

    Energy Technology Data Exchange (ETDEWEB)

    Himpel, Michael, E-mail: himpel@physik.uni-greifswald.de; Killer, Carsten; Melzer, André [Institute of Physics, Ernst-Moritz-Arndt-University, 17489 Greifswald (Germany); Bockwoldt, Tim; Piel, Alexander [IEAP, Christian-Albrechts-Universität Kiel, D-24098 Kiel (Germany); Ole Menzel, Kristoffer [ABB Switzerland Ltd, Corporate Research Center, 5405 Dättwil (Switzerland)

    2014-03-15

    Experiments on dust-density waves have been performed in dusty plasmas under the microgravity conditions of parabolic flights. Three-dimensional measurements of a dust density wave on a single particle level are presented. The dust particles have been tracked for many oscillation periods. A Hilbert analysis is applied to obtain trajectory parameters such as oscillation amplitude and three-dimensional velocity amplitude. While the transverse motion is found to be thermal, the velocity distribution in wave propagation direction can be explained by harmonic oscillations with added Gaussian (thermal) noise. Additionally, it is shown that the wave properties can be reconstructed by means of a pseudo-stroboscopic approach. Finally, the energy dissipation mechanism from the kinetic oscillation energy to thermal motion is discussed and presented using phase-resolved analysis.

  6. Connecting diffusion and entropy of bulk water at the single particle ...

    Indian Academy of Sciences (India)

    The relation between the dynamic (e.g., diffusion) and thermodynamic (e.g., entropy) properties of water and water-like liquids has been an active area of research for a long time. Although several studies have investigated the diffusivity and entropy for different systems, these studies have probed either the configurational ...

  7. Quantitative Single-Particle Digital Autoradiography with α-Particle Emitters for Targeted Radionuclide Therapy using the iQID Camera

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Brian W.; Frost, Sophia; Frayo, Shani; Kenoyer, Aimee L.; Santos, E. B.; Jones, Jon C.; Green, Damian J.; Hamlin, Donald K.; Wilbur, D. Scott; Fisher, Darrell R.; Orozco, Johnnie J.; Press, Oliver W.; Pagel, John M.; Sandmaier, B. M.

    2015-07-01

    ). Estimation of the 211At activity distribution was demonstrated at mBq/μg levels. Conclusion: Single-particle digital autoradiography of alpha emitters has advantages over traditional autoradiographic techniques in terms of spatial resolution, sensitivity, and activity quantification capability. The system features and characterization results presented in this study show that iQID is a promising technology for microdosimetry, because it provides necessary information for interpreting alpha-RIT outcomes and for predicting the therapeutic efficacy of cell-targeted approaches using alpha emitters.

  8. Experimental study of the rearrangements of valence protons and neutrons amongst single-particle orbits during double- β decay in Mo100

    Energy Technology Data Exchange (ETDEWEB)

    Freeman, S. J.; Sharp, D. K.; McAllister, S. A.; Kay, B. P.; Deibel, C. M.; Faestermann, T.; Hertenberger, R.; Mitchell, A. J.; Schiffer, J. P.; Szwec, S. V.; Thomas, J. S.; Wirth, H. -F.

    2017-11-01

    The rearrangements of protons and neutrons amongst the valence single-particle orbitals during double-beta decay of Mo-100 have been determined by measuring cross sections in (d, p), (p, d), (He-3, a), and (He-3, d) reactions on Mo-98,Mo-100 and Ru-100,Ru-102 targets. The deduced nucleon occupancies reveal significant discrepancies when compared with theoretical calculations; the same calculations have previously been used to determine the nuclear matrix element associated with the decay probability of double-beta decay of the Mo-100 system.

  9. Lipid diffusion in the distal and proximal leaflets of supported lipid bilayer membranes studied by single particle tracking

    Science.gov (United States)

    Schoch, Rafael L.; Barel, Itay; Brown, Frank L. H.; Haran, Gilad

    2018-03-01

    Supported lipid bilayers (SLBs) have been studied extensively as simple but powerful models for cellular membranes. Yet, potential differences in the dynamics of the two leaflets of a SLB remain poorly understood. Here, using single particle tracking, we obtain a detailed picture of bilayer dynamics. We observe two clearly separate diffusing populations, fast and slow, that we associate with motion in the distal and proximal leaflets of the SLB, respectively, based on fluorescence quenching experiments. We estimate diffusion coefficients using standard techniques as well as a new method based on the blur of images due to motion. Fitting the observed diffusion coefficients to a two-leaflet membrane hydrodynamic model allows for the simultaneous determination of the intermonolayer friction coefficient and the substrate-membrane friction coefficient, without any prior assumptions on the strengths of the relevant interactions. Remarkably, our calculations suggest that the viscosity of the interfacial water confined between the membrane and the substrate is elevated by ˜104 as compared to bulk water. Using hidden Markov model analysis, we then obtain insight into the transbilayer movement of lipids. We find that lipid flip-flop dynamics are very fast, with half times in the range of seconds. Importantly, we find little evidence for membrane defect mediated lipid flip-flop for SLBs at temperatures well above the solid-to-liquid transition, though defects seem to be involved when the SLBs are cooled down. Our work thus shows that the combination of single particle tracking and advanced hydrodynamic modeling provides a powerful means to obtain insight into membrane dynamics.

  10. Online Characterisation of Mineral Dust Aerosol by Single Particle Mass Spectrometry: Mineralogical Signatures of Potential Source Areas in North Africa.

    Science.gov (United States)

    Marsden, N. A.; Allan, J. D.; Flynn, M.; Ullrich, R.; Moehler, O.; Coe, H.

    2017-12-01

    The mineralogy of individual dust particles is important for atmospheric processes because mineralogy influences optical properties, their potential to act as ice nucleating particles (INP) and geochemical cycling of elements to the ocean. Bulk mineralogy of transported mineral dust has been shown to be a reflection of the source area and size fractionation during transport. Online characterisation of single particle mineralogy is highly desirable as the composition of individual particles can be reported at a temporal resolution that is relevant to atmospheric processes. Single particle mass spectrometry (SPMS) has indentified and characterised the composition of ambient dust particles but is hampered by matrix effects that result in a non-quantatative measurement of composition. The work presented describes a comparison of mass spectral characteristics of sub 2.5μm particle fractions generated from; i) nominally pure samples from the clay mineral society (CMS), ii) soil samples collected from potential source areas in North Africa and iii) ambient measurement of transported African dust made at the Cape Verde Islands. Using a novel approach to analyse the mass spectra, the distinct characteristics of the various dust samples are obtained from the online measurements. Using this technique it was observed that dust generated from sources on the North West Margin of the Sahara Desert have distinct characteristics of illite in contrast to the kaolinitic characteristics of dust generated from sources in the Sahel. These methods offer great potential for describing the hourly variation in the source and mineralogy of transported mineral dust and the online differentiation of mineral phase in multi-mineralic dust samples.

  11. Single-particle cryo-EM using alignment by classification (ABC): the structure ofLumbricus terrestrishaemoglobin.

    Science.gov (United States)

    Afanasyev, Pavel; Seer-Linnemayr, Charlotte; Ravelli, Raimond B G; Matadeen, Rishi; De Carlo, Sacha; Alewijnse, Bart; Portugal, Rodrigo V; Pannu, Navraj S; Schatz, Michael; van Heel, Marin

    2017-09-01

    Single-particle cryogenic electron microscopy (cryo-EM) can now yield near-atomic resolution structures of biological complexes. However, the reference-based alignment algorithms commonly used in cryo-EM suffer from reference bias, limiting their applicability (also known as the 'Einstein from random noise' problem). Low-dose cryo-EM therefore requires robust and objective approaches to reveal the structural information contained in the extremely noisy data, especially when dealing with small structures. A reference-free pipeline is presented for obtaining near-atomic resolution three-dimensional reconstructions from heterogeneous ('four-dimensional') cryo-EM data sets. The methodologies integrated in this pipeline include a posteriori camera correction, movie-based full-data-set contrast transfer function determination, movie-alignment algorithms, (Fourier-space) multivariate statistical data compression and unsupervised classification, 'random-startup' three-dimensional reconstructions, four-dimensional structural refinements and Fourier shell correlation criteria for evaluating anisotropic resolution. The procedures exclusively use information emerging from the data set itself, without external 'starting models'. Euler-angle assignments are performed by angular reconstitution rather than by the inherently slower projection-matching approaches. The comprehensive 'ABC-4D' pipeline is based on the two-dimensional reference-free 'alignment by classification' (ABC) approach, where similar images in similar orientations are grouped by unsupervised classification. Some fundamental differences between X-ray crystallography versus single-particle cryo-EM data collection and data processing are discussed. The structure of the giant haemoglobin from Lumbricus terrestris at a global resolution of ∼3.8 Å is presented as an example of the use of the ABC-4D procedure.

  12. Single particle aerosol mass spectrometry of coal combustion particles associated with high lung cancer rates in Xuanwei and Fuyuan, China.

    Science.gov (United States)

    Lu, Senlin; Tan, Zhengying; Liu, Pinwei; Zhao, Hui; Liu, Dingyu; Yu, Shang; Cheng, Ping; Win, Myat Sandar; Hu, Jiwen; Tian, Linwei; Wu, Minghong; Yonemochi, Shinich; Wang, Qingyue

    2017-11-01

    Coal combustion particles (CCPs) are linked to the high incidence of lung cancer in Xuanwei and in Fuyuan, China, but studies on the chemical composition of the CCPs are still limited. Single particle aerosol mass spectrometry (SPAMS) was recently developed to measure the chemical composition and size of single particles in real-time. In this study, SPAMS was used to measure individual combustion particles emitted from Xuanwei and Fuyuan coal samples and the results were compared with those by ICP-MS and transmission electron microscopy (TEM). The total of 38,372 particles mass-analyzed by SPAMS can be divided into 9 groups based on their chemical composition and their number percentages: carbonaceous, Na-rich, K-rich, Al-rich, Fe-rich, Si-rich, Ca-rich, heavy metal-bearing, and PAH-bearing particles. The carbonaceous and PAH-bearing particles are enriched in the size range below 0.56 μm, Fe-bearing particles range from 0.56 to 1.0 μm in size, and heavy metals such as Ti, V, Cr, Cu, Zn, and Pb have diameters below 1 μm. The TEM results show that the particles from Xuanwei and Fuyuan coal combustion can be classified into soot aggregates, Fe-rich particles, heavy metal containing particles, and mineral particles. Non-volatile particles detected by SPAMS could also be observed with TEM. The number percentages by SPAMS also correlate with the mass concentrations measured by ICP-MS. Our results could provide valuable insight for understanding high lung cancer incidence in the area. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. A wavelength tunable ONU transmitter based on multi-mode Fabry-Perot laser and micro-ring resonator for bandwidth symmetric TWDM-PON

    Science.gov (United States)

    Gao, Zhensen; Sun, Xiao; Zhang, Kaibin

    2016-02-01

    Wavelength tunable optical transmitter is an essential component for the newly standardized time and wavelength division multiplexed passive optical network (TWDM-PON), where tunable ONU with 10Gb/s bit rate is desired to provide 40Gb/s symmetric bandwidth. In this paper, a novel wavelength tunable optical transmitter is proposed by reusing legacy low speed multi-mode Fabry-Perot laser and connecting it with an integrated photonic chip with two coupled micro-ring resonators to generate a tunable single mode signal based on Vernier effect for 10Gb/s high speed modulation, which makes it as a promising solution for colorless ONU in future symmetric TWDM-PON.

  14. Characterization of single particle aerosols by elastic light scattering at multiple wavelengths

    Science.gov (United States)

    Lane, P. A.; Hart, M. B.; Jain, V.; Tucker, J. E.; Eversole, J. D.

    2018-03-01

    We describe a system to characterize individual aerosol particles using stable and repeatable measurement of elastic light scattering. The method employs a linear electrodynamic quadrupole (LEQ) particle trap. Charged particles, continuously injected by electrospray into this system, are confined to move vertically along the stability line in the center of the LEQ past a point where they are optically interrogated. Light scattered in the near forward direction was measured at three different wavelengths using time-division multiplexed collinear laser beams. We validated our method by comparing measured silica microsphere data for four selected diameters (0.7, 1.0, 1.5 and 2.0 μm) to a model of collected scattered light intensities based upon Lorenz-Mie scattering theory. Scattered light measurements at the different wavelengths are correlated, allowing us to distinguish and classify inhomogeneous particles.

  15. Qualitatively different collective and single particle dynamics in a supercooled liquid

    OpenAIRE

    Priya, Madhu; Bidhoodi, Neeta; Das, Shankar P.

    2015-01-01

    The equations of fluctuating nonlinear hydrodynamics for a two component mixture are obtained with a proper choice of slow variables which correspond to the conservation laws in the system. Using these nonlinear equations we construct the basic equations of the mode coupling theory (MCT) and consequent ergodic-nonergodic (ENE) transition in a binary mixture. The model is also analyzed in the one component limit of the mixture to study the dynamics of a tagged particle in the sea of identical ...

  16. Creation of giant two-dimensional crystal of zinc oxide nanodisk by method of single-particle layer of organo-modified inorganic fine particles.

    Science.gov (United States)

    Meng, Qi; Honda, Nanami; Uchida, Saki; Hashimoto, Kazuaki; Shibata, Hirobumi; Fujimori, Atsuhiro

    2015-09-01

    In this study, the formation and structure of a single-particle layer of organo-zinc oxide are investigated using surface-pressure-area (π-A) isotherms, out-of-plane X-ray diffraction (XRD) analysis, and atomic force microscopy (AFM). Further, techniques for achieving the solubilization of inorganic fine particles in general solvents have been proposed, and a single-particle layer has been formed using such an inorganic solution as a "spreading solution" for an interfacial film. Surface modification of ZnO is performed using a long-chain carboxylic acid. Accordingly, a regular arrangement of ZnO can be easily achieved in order to overcome the relatively weak van der Walls interactions between inorganic materials. A condensed Langmuir monolayer of these particles is also formed. A multiparticle layered structure is constructed by the Langmuir-Blodgett (LB) technique. Out-of-plane XRD measurement results for a single-particle layer of organo-ZnO clearly show a sharp peak at 42 Å. This peak is attributed to the distance between ZnO layers. The AFM image of this single-particle layer of organo-ZnO shows a particle assembly with a uniform height of 60 nm. These aggregated particles form large two-dimensional crystals. In other words, a regular periodic structure along the c-axis and a condensed single-particle layer had been fabricated using Langmuir and LB techniques. Copyright © 2015 Elsevier Inc. All rights reserved.

  17. Cantilever-based micro-particle filter with simultaneous single particle detection

    DEFF Research Database (Denmark)

    Noeth, Nadine-Nicole; Keller, Stephan Sylvest; Boisen, Anja

    2011-01-01

    -particles from a liquid. A hole-array is integrated into a micro-cantilever, which is inserted into a microfluidic channel perpendicular to the flow. A metal pad at the apex of the cantilever enables an optical read-out of the deflection of the cantilever. When a micro-particle is too large to pass a hole...... in the cantilever, clogging of the holes increases the flow resistance of the cantilever. This causes a bending of the device, which can be detected by the optical read-out system. By arranging an array of such cantilevers with different hole sizes, separation by size can be achieved. In this paper a proof...

  18. Interplay between collective and single particle excitations around neutron-rich doubly-magic nuclei

    Directory of Open Access Journals (Sweden)

    Leoni S.

    2016-01-01

    Full Text Available The excitation spectra of nuclei with one or two particles outside a doubly-magic core are expected to be dominated, at low energy, by the couplings between phonon excitations of the core and valence particles. A survey of the experimental situation is given for some nuclei lying in close proximity of neutron-rich doubly-magic systems, such as 47,49Ca, 133Sb and 210Bi. Data are obtained with various types of reactions (multinucleon transfer with heavy ions, cold neutron capture and neutron induced fission of 235U and 241Pu targets, with the employment of complex detection systems based on HPGe arrays. A comparison with theoretical calculations is also presented, in terms of large shell model calculations and of a phenomenological particle-phonon model. In the case of 133Sb, a new microscopic “hybrid” model is introduced: it is based on the coupling between core excitations (both collective and non-collective of the doubly-magic core and the valence nucleon, using the Skyrme effective interaction in a consistent way.

  19. High-contrast single-particle tracking by selective focal plane illumination microscopy.

    Science.gov (United States)

    Ritter, Jörg G; Veith, Roman; Siebrasse, Jan-Peter; Kubitscheck, Ulrich

    2008-05-12

    Wide-field single molecule microscopy is a versatile tool for analyzing dynamics and molecular interactions in biological systems. In extended three-dimensional systems, however, the method suffers from intrinsic out-of-focus fluorescence. We constructed a high-resolution selective plane illumination microscope (SPIM) to efficiently solve this problem. The instrument is an optical sectioning microscope featuring the high speed and high sensitivity of a video microscope. We present theoretical calculations and quantitative measurements of the illumination light sheet thickness yielding 1.7 microm (FWHM) at 543 nm, 2.0 microm at 633 nm, and a FWHM of the axial point spread function of 1.13 microm. A direct comparison of selective plane and epi-illumination of model samples with intrinsic background fluorescence illustrated the clear advantage of SPIM for such samples. Single fluorescent quantum dots in aqueous solution are readily visualized and tracked proving the suitability of our setup for the study of fast and dynamic processes in spatially extended biological specimens.

  20. Intracellular dynamics and fate of polystyrene nanoparticles in A549 Lung epithelial cells monitored by image (cross-) correlation spectroscopy and single particle tracking.

    Science.gov (United States)

    Deville, Sarah; Penjweini, Rozhin; Smisdom, Nick; Notelaers, Kristof; Nelissen, Inge; Hooyberghs, Jef; Ameloot, Marcel

    2015-10-01

    Novel insights in nanoparticle (NP) uptake routes of cells, their intracellular trafficking and subcellular targeting can be obtained through the investigation of their temporal and spatial behavior. In this work, we present the application of image (cross-) correlation spectroscopy (IC(C)S) and single particle tracking (SPT) to monitor the intracellular dynamics of polystyrene (PS) NPs in the human lung carcinoma A549 cell line. The ensemble kinetic behavior of NPs inside the cell was characterized by temporal and spatiotemporal image correlation spectroscopy (TICS and STICS). Moreover, a more direct interpretation of the diffusion and flow detected in the NP motion was obtained by SPT by monitoring individual NPs. Both techniques demonstrate that the PS NP transport in A549 cells is mainly dependent on microtubule-assisted transport. By applying spatiotemporal image cross-correlation spectroscopy (STICCS), the correlated motions of NPs with the early endosomes, late endosomes and lysosomes are identified. PS NPs were equally distributed among the endolysosomal compartment during the time interval of the experiments. The cotransport of the NPs with the lysosomes is significantly larger compared to the other cell organelles. In the present study we show that the complementarity of ICS-based techniques and SPT enables a consistent elaborate model of the complex behavior of NPs inside biological systems. Copyright © 2015 Elsevier B.V. All rights reserved.

  1. Clathrin coat disassembly by the yeast Hsc70/Ssa1p and auxilin/Swa2p proteins observed by single-particle burst analysis spectroscopy.

    Science.gov (United States)

    Krantz, Kelly C; Puchalla, Jason; Thapa, Rajan; Kobayashi, Callie; Bisher, Margaret; Viehweg, Julie; Carr, Chavela M; Rye, Hays S

    2013-09-13

    The role of clathrin-coated vesicles in receptor-mediated endocytosis is conserved among eukaryotes, and many of the proteins required for clathrin coat assembly and disassembly have orthologs in yeast and mammals. In yeast, dozens of proteins have been identified as regulators of the multistep reaction required for endocytosis, including those that regulate disassembly of the clathrin coat. In mammalian systems, clathrin coat disassembly has been reconstituted using neuronal clathrin baskets mixed with the purified chaperone ATPase 70-kDa heat shock cognate (Hsc70), plus a clathrin-specific co-chaperone, such as the synaptic protein auxilin. Yet, despite previous characterization of the yeast Hsc70 ortholog, Ssa1p, and the auxilin-like ortholog, Swa2p, testing mechanistic models for disassembly of nonneuronal clathrin coats has been limited by the absence of a functional reconstitution assay. Here we use single-particle burst analysis spectroscopy, in combination with fluorescence correlation spectroscopy, to follow the population dynamics of fluorescently tagged yeast clathrin baskets in the presence of purified Ssa1p and Swa2p. An advantage of this combined approach for mechanistic studies is the ability to measure, as a function of time, changes in the number and size of objects from a starting population to the reaction products. Our results indicate that Ssa1p and Swa2p cooperatively disassemble yeast clathrin baskets into fragments larger than the individual triskelia, suggesting that disassembly of clathrin-coated vesicles may proceed through a partially uncoated intermediate.

  2. Compatibility of localized wave packets and unrestricted single particle dynamics for cluster formation in nuclear collisions

    International Nuclear Information System (INIS)

    Ono, A.

    2002-03-01

    Anti-symmetrized molecular dynamics with quantum branching is generalized so as to allow finite time duration of the unrestricted coherent mean field propagation which is followed by the decoherence into wave packets. In this new model, the wave packet shrinking by the mean field propagation is respected as well as the diffusion, so that it predicts a one-body dynamics similar to that in mean field models. The shrinking effect is expected to change the diffusion property of nucleons in nuclear matter and the global one-body dynamics. The central 129 Xe + Sn collisions at 50 MeV/nucleon are calculated by the models with and without shrinking, and it is shown that the inclusion of the wave packet shrinking has a large effect on the multifragmentation in a big expanding system with a moderate expansion velocity. (author)

  3. Analysis of suspended solids by single-particle scattering. [for Lake Superior pollution monitoring

    Science.gov (United States)

    Diehl, S. R.; Smith, D. T.; Sydor, M.

    1979-01-01

    Light scattering by individual particulates is used in a multiple-detector system to categorize the composition of suspended solids in terms of broad particulate categories. The scattering signatures of red clay and taconite tailings, the two primary particulate contaminants in western Lake Superior, along with two types of asbestiform fibers, amphibole and chrysolite, were studied in detail. A method was developed to predict the concentration of asbestiform fibers in filtration plant samples for which electron microscope analysis was done concurrently. Fiber levels as low as 50,000 fibers/liter were optically detectable. The method has application in optical categorization of samples for remote sensing purposes and offers a fast, inexpensive means for analyzing water samples from filtration plants for specific particulate contaminants.

  4. Electrodynamic balance-mass spectrometry of single particles as a new platform for atmospheric chemistry research

    Science.gov (United States)

    Birdsall, Adam W.; Krieger, Ulrich K.; Keutsch, Frank N.

    2018-01-01

    New analytical techniques are needed to improve our understanding of the intertwined physical and chemical processes that affect the composition of aerosol particles in the Earth's atmosphere, such as gas-particle partitioning and homogenous or heterogeneous chemistry, and their ultimate relation to air quality and climate. We describe a new laboratory setup that couples an electrodynamic balance (EDB) to a mass spectrometer (MS). The EDB stores a single laboratory-generated particle in an electric field under atmospheric conditions for an arbitrarily long length of time. The particle is then transferred via gas flow to an ionization region that vaporizes and ionizes the analyte molecules before MS measurement. We demonstrate the feasibility of the technique by tracking evaporation of polyethylene glycol molecules and finding agreement with a kinetic model. Fitting data to the kinetic model also allows determination of vapor pressures to within a factor of 2. This EDB-MS system can be used to study fundamental chemical and physical processes involving particles that are difficult to isolate and study with other techniques. The results of such measurements can be used to improve our understanding of atmospheric particles.

  5. Single-particle effects in fine structure of super-asymmetric fission

    International Nuclear Information System (INIS)

    Mirea, M.

    1999-01-01

    Energy spectrum measurements concerning the 14 C decay from 223 Ra revealed a fine structure with an intense branch on the excited state of the daughter 209 Pb. Apart the great number of microscopic--macroscopic attempts of different authors in describing this behavior (compiled recently), this phenomenon was explained quantitatively using the Landau--Zener effect, i.e., the promotion mechanism of a unpaired nucleon between two levels characterised by the same quantum numbers connected to some symmetries of the nuclear system in the region where an avoided level crossing is exhibited. The adiabatic levels during the super-asymmetric fission process were determined with a new version of the two--centre shell model especially constructed for very large mass--asymmetries. The half--lives are obtained in the framework of the Wentzel--Kramers--Brillouin approximation. The amount of the variation of the barrier height in the excited channels was estimated accounting the specialization energy which can be interpreted as the excess of the energy of a nucleon with a given spin over the energy for the same spin nucleon state of lowest energy. It is evidenced that the fine structure of cluster decay is due to two competitive effects: the Landau--Zener effect which enhances the probability to have an excited daughter in the final channel and the specialization energy which increases the potential barrier and therefore leads to a diminution of the penetrability. This formalism was used for predictions of the fine structure in the case of 14 C decay of 225 Ac and to explain the fine structure of alpha decay. (author)

  6. Behavior of Windblown Sand on Mars: Results from Single-Particle Experiments

    Science.gov (United States)

    Marshall, J. R.; Borucki, J.; Sagan, C.

    1999-01-01

    Experiments are investigating the behavior of individual sand grains in the high-energy martian aeolian regime. Energy partitioning during impact of a saltating grain determines grain longevity, but it also influences the way in which the bed becomes mobilized by reptation. When single grains of sand are fired into loose beds, the bed can absorb up to 90% of the impact energy by momentum transfer to other grains; it has been discovered that the impacting grains cause circular craters even at low impact angles. Hundreds of grains can be splashed by a single high-velocity (100 m/s) impact causing more bed disturbance through reptation than previously thought. The research is supported by NASA's PG&G Program. Because the martian aeolian environment in both high energy and of long duration, the most mobile fractions of windblown sand should have eradicated themselves by attrition, unless sand supply has kept pace with destruction. It is therefore important to understand the rate of grain attrition in order to make sense of the existence of vast dune fields on Mars. Attrition, has been addressed in other studies, but precise data for a single saltating grain striking a loose bed of sand have not been acquired -- the quintessential case to be understood for dunes on Mars. To acquire these data, we are employing a compound crossbow which has the bolt-firing mechanism replaced with a pneumatically-automated sabot system. The sabot can launch individual grains of sand of any size between several millimeters and about 50 microns, at velocities up to 100m/s. This is around the maximum velocity expected for saltating grains on Mars. The sabot sled is equipped with photoelectric sensors for measuring shot velocity. Baffling of the grain's exit orifice has enabled projection of single grains without significant aerodynamic effects from the sabot. Grains are fired into loose beds of sand at about 15 degrees from the horizontal (typical saltation trajectory at impact) while being

  7. Influence of gold coating and interplate voltage on the performance of chevron micro-channel plates for temporally and spatially resolved single particle detection

    Science.gov (United States)

    Hoendervanger, A. L.; Clément, D.; Aspect, A.; Westbrook, C. I.; Dowek, D.; Picard, Y. J.; Boiron, D.

    2013-02-01

    We present a study of two different sets of Micro-Channel Plates used for time and space resolved single particle detection. We investigate the effects of the gold coating and that of introducing an interplate voltage between the spatially separated plates. We find that the gold coating increases the count rate of the detector and the pulse amplitude as previously reported for non-spatially resolved setups. The interplate voltage also increases count rates. In addition, we find that a non-zero interplate voltage improves the spatial accuracy in determining the arrival position of incoming single particles (by ˜20%) while the gold coating has a negative effect (by ˜30%).

  8. Transport in Weakly Coupled Vertical Double Quantum Dots: Single-Particle Energy Level Spectroscopy and Hyperfine Interaction Effects

    Science.gov (United States)

    Payette, Christopher

    2011-12-01

    Performing transport measurements on weakly coupled vertical double quantum dots, we study by magneto-resonant-tunneling spectroscopy, single-particle energy spectra of the constituent dots over a wide energy window. The measured energy spectra are well modeled overall by ideal spectra calculated for elliptical and parabolic in-dot-plane confinement potentials. However, in regions where single-particle energy levels are naively expected to cross, we observe pronounced level anti-crossing behaviour and strong resonant current variations (both enhancement and suppression). Within a coherent tunneling picture, these effects can be attributed to coherent level mixing induced by weak perturbations in the nearly ideal dot confinement potentials. We analyze the energy spectra in detail, and focus on examples of two-, three- and four-level crossings where we observe the suppression of an otherwise strong current resonance, a signature of dark state formation due to destructive interference. The mixing we measure and model at two three-level crossings represents an all-electrical analogue of coherent population trapping. We also explore the limitations of the applicability of the coherent level mixing model and demonstrate in-situ alteration of the coupling between levels. We further examine the electron spin-nuclear spin (hyperfine) interaction. In the familiar two-electron spin blockade regime, on application of an out-of-dot-plane magnetic field, we observe current switching and hysteresis, and a funnel-like structure in the leakage current, all hallmarks of the hyperfine interaction. The measurements bring to light a strong gate voltage dependence, significant device-to-device variations, and an intricate bias voltage history dependence not accounted for in any existing model. Unexpectedly, we also observe signatures of the hyperfine interaction at high bias, well outside the spin blockade regime. We characterize these features and suggest how the hyperfine interaction

  9. Molecular dynamics simulations of the structure and single-particle dynamics of mixtures of divalent salts and ionic liquids

    Science.gov (United States)

    Gómez-González, Víctor; Docampo-Álvarez, Borja; Cabeza, Oscar; Fedorov, Maxim; Lynden-Bell, Ruth M.; Gallego, Luis J.; Varela, Luis M.

    2015-09-01

    We report a molecular dynamics study of the structure and single-particle dynamics of mixtures of a protic (ethylammonium nitrate) and an aprotic (1-butyl-3-methylimidazolium hexaflurophosphate [BMIM][PF6]) room-temperature ionic liquids doped with magnesium and calcium salts with a common anion at 298.15 K and 1 atm. The solvation of these divalent cations in dense ionic environments is analyzed by means of apparent molar volumes of the mixtures, radial distribution functions, and coordination numbers. For the protic mixtures, the effect of salt concentration on the network of hydrogen bonds is also considered. Moreover, single-particle dynamics of the salt cations is studied by means of their velocity autocorrelation functions and vibrational densities of states, explicitly analyzing the influence of salt concentration, and cation charge and mass on these magnitudes. The effect of the valency of the salt cation on these properties is considered comparing the results with those for the corresponding mixtures with lithium salts. We found that the main structural and dynamic features of the local solvation of divalent cations in ionic liquids are similar to those of monovalent salts, with cations being localized in the polar nanoregions of the bulk mixture coordinated in monodentate and bidentate coordination modes by the [NO3]- and [PF6]- anions. However, stronger electrostatic correlations of these polar nanoregions than in mixtures with salts with monovalent cations are found. The vibrational modes of the ionic liquid (IL) are seen to be scarcely affected by the addition of the salt, and the effect of mass and charge on the vibrational densities of states of the dissolved cations is reported. Cation mass is seen to exert a deeper influence than charge on the low-frequency vibrational spectra, giving a red shift of the vibrational modes and a virtual suppression of the higher energy vibrational modes for the heavier Ca2+ cations. No qualitative difference with

  10. Pedagogical Comparison of Five Reactions Performed under Microwave Heating in Multi-Mode versus Mono-Mode Ovens: Diels-Alder Cycloaddition, Wittig Salt Formation, E2 Dehydrohalogenation to Form an Alkyne, Williamson Ether Synthesis, and Fischer Esterification

    Science.gov (United States)

    Baar, Marsha R.; Gammerdinger, William; Leap, Jennifer; Morales, Erin; Shikora, Jonathan; Weber, Michael H.

    2014-01-01

    Five reactions were rate-accelerated relative to the standard reflux workup in both multi-mode and mono-mode microwave ovens, and the results were compared to determine whether the sequential processing of a mono-mode unit could provide for better lab logistics and pedagogy. Conditions were optimized so that yields matched in both types of…

  11. Wideband, Low-power Multi-mode MMIC Radar Transceivers with Phase Control and Integrated Baseband Signal Processing, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — NASA has numerous missions that involve radar and radiometry. In the past, the practice has been to build each system as a one-off program, which makes MMIC design...

  12. Cryptanalysis and Improvement for the Quantum Private Comparison Protocol Based on Triplet Entangled State and Single-Particle Measurement

    Science.gov (United States)

    Ting, Xu; Tian-Yu, Ye

    2017-03-01

    Quantum private comparison (QPC) aims to accomplish the equality comparison of secret inputs from two users on the basis of not leaking their contents out. Recently, Chen et al. proposed the QPC protocol based on triplet GHZ state and single-particle measurement (Optics Communications 283, 1561-1565 (2010)). In this paper, they suggested the standard model of a semi-honest third party (TP) for the first time, and declared that their protocol is secure. Subsequently, Lin et al. pointed out that in Chen et al.'s protocol, one user can extract the other user's secret without being discovered by performing the intercept-resend attack, and suggested two corresponding improvements (Optics Communications 284, 2412-2414 (2011)). However, Yang et al. first pointed out that the model of TP adopted by both Chen et al.'s protocol and Lin et al.'s improved protocols is unreasonable, and thought that a practical TP may also try any possible means to steal the users' secrets except being corrupted by the adversary including the dishonest user (Quantum Inf Process 12, 877-885 (2013). In this paper, after taking the possible attacks from TP into account, we propose the eavesdropping strategy of TP toward Lin et al.'s improved protocols and suggest two feasible solutions accordingly.

  13. HybTrack: A hybrid single particle tracking software using manual and automatic detection of dim signals.

    Science.gov (United States)

    Lee, Byung Hun; Park, Hye Yoon

    2018-01-09

    Single particle tracking is a compelling technique for investigating the dynamics of nanoparticles and biological molecules in a broad range of research fields. In particular, recent advances in fluorescence microscopy have made single molecule tracking a prevalent method for studying biomolecules with a high spatial and temporal precision. Particle tracking algorithms have matured over the past three decades into more easily accessible platforms. However, there is an inherent difficulty in tracing particles that have a low signal-to-noise ratio and/or heterogeneous subpopulations. Here, we present a new MATLAB based tracking program which combines the benefits of manual and automatic tracking methods. The program prompts the user to manually locate a particle when an ambiguous situation occurs during automatic tracking. We demonstrate the utility of this program by tracking the movement of β-actin mRNA in the dendrites of cultured hippocampal neurons. We show that the diffusion coefficient of β-actin mRNA decreases upon neuronal stimulation by bicuculline treatment. This tracking method enables an efficient dissection of the dynamic regulation of biological molecules in highly complex intracellular environments.

  14. Effect of particle shape and structure on the results of single-particle light-scattering size analysis.

    Science.gov (United States)

    Umhauer, H; Bottlinger, M

    1991-11-20

    To evaluate quantitatively the influence exerted by the shape and structure of nonspherical, nonideal particles on the results of single-particle scattered-light size analysis, measurements were conducted with individual particles of different materials (glass, limestone, and quartz). For this purpose, the particles were suspended in an electrodynamic balance and repeatedly passed through the analyzer's measuring volume with a continually changing random orientation. The scattered-light signal spectra thus obtained specify the probability with which a certain pulse height is induced when the particle passes once through the measuring volume at a given coincidental orientation. The spectra reflect the material-characteristic influence. They allow the loss of resolution of common scattered-light size analyses to be assessed and algorithms (matrices) to be compiled with which the shape and structure influence may be mathematically eliminated. Because a shape and structure independent size parameter is also determined from the individual particles, exact calibration curves can be derived in which the shape and structure influence are incorporated.

  15. Single Particle and PET-based Platform for Identifying Optimal Plasmonic Nano-Heaters for Photothermal Cancer Therapy.

    Science.gov (United States)

    Jørgensen, Jesper Tranekjær; Norregaard, Kamilla; Tian, Pengfei; Bendix, Poul Martin; Kjaer, Andreas; Oddershede, Lene B

    2016-08-02

    Plasmonic nanoparticle-based photothermal cancer therapy is a promising new tool to inflict localized and irreversible damage to tumor tissue by hyperthermia, without harming surrounding healthy tissue. We developed a single particle and positron emission tomography (PET)-based platform to quantitatively correlate the heat generation of plasmonic nanoparticles with their potential as cancer killing agents. In vitro, the heat generation and absorption cross-section of single irradiated nanoparticles were quantified using a temperature sensitive lipid-based assay and compared to their theoretically predicted photo-absorption. In vivo, the heat generation of irradiated nanoparticles was evaluated in human tumor xenografts in mice using 2-deoxy-2-[F-18]fluoro-D-glucose ((18)F-FDG) PET imaging. To validate the use of this platform, we quantified the photothermal efficiency of near infrared resonant silica-gold nanoshells (AuNSs) and benchmarked this against the heating of colloidal spherical, solid gold nanoparticles (AuNPs). As expected, both in vitro and in vivo the heat generation of the resonant AuNSs performed superior compared to the non-resonant AuNPs. Furthermore, the results showed that PET imaging could be reliably used to monitor early treatment response of photothermal treatment. This multidisciplinary approach provides a much needed platform to benchmark the emerging plethora of novel plasmonic nanoparticles for their potential for photothermal cancer therapy.

  16. Spatial filtering technique to image and measure two-dimensional near-forward scattering from single particles.

    Science.gov (United States)

    Berg, Matthew J; Hill, Steven C; Videen, Gorden; Gurton, Kristan P

    2010-04-26

    This work describes the design and use of an optical apparatus to measure the far-field elastic light-scattering pattern for a single particle over two angular-dimensions. A spatial filter composed of a mirror with a small through-hole is used to enable collection of the pattern uncommonly close to the forward direction; to within tenths of a degree. Minor modifications of the design allow for the simultaneous measurement of a particle's image along with its two-dimensional scattering pattern. Example measurements are presented involving single micrometer-sized glass spherical particles confined in an electrodynamic trap and a dilute suspension of polystyrene latex particles in water. A small forward-angle technique, called Guinier analysis, is used to determine a particle-size estimate directly from the measured pattern without a priori knowledge of the particle refractive index. Comparison of these size estimates to those obtained by fitting the measurements to Mie theory reveals relative errors low as 2%.

  17. Detection and Sizing of Ti-Containing Particles in Recreational Waters Using Single Particle ICP-MS.

    Science.gov (United States)

    Venkatesan, Arjun K; Reed, Robert B; Lee, Sungyun; Bi, Xiangyu; Hanigan, David; Yang, Yu; Ranville, James F; Herckes, Pierre; Westerhoff, Paul

    2018-01-01

    Single particle inductively coupled plasma mass spectrometry (spICP-MS) was used to detect Ti-containing particles in heavily-used bathing areas of a river (Salt River) and five swimming pools. Ti-containing particle concentrations in swimming pools ranged from 2.8 × 10 3 to 4.4 × 10 3 particles/mL and were an order of magnitude lower than those detected in the Salt River. Measurements from the Salt River showed an 80% increase in Ti-containing particle concentration over baseline concentration during peak recreational activity (at 16:00 h) in the river. Cloud point extraction followed by transmission electron microscopy with energy dispersive X-ray analysis confirmed presence of aggregated TiO 2 particles in river samples, showing morphological similarity to particles present in an over-the-counter sunscreen product. The maximum particle mass concentration detected in a sample from the Salt River (659 ng/L) is only slightly lower than the predicted no effect concentration for TiO 2 to aquatic organisms (< 1 μg/L).

  18. International interlaboratory study for sizing and quantification of Ag nanoparticles in food simulants by single-particle ICPMS.

    Science.gov (United States)

    Linsinger, Thomas P J; Peters, Ruud; Weigel, Stefan

    2014-06-01

    This publication describes the first international intercomparison of particle-size determination by single-particle inductively coupled plasma mass spectrometry (sp-ICPMS). Concentrated monodisperse silver nanoparticle suspensions with particle diameters of 20, 40 and 100 nm and a blank solution were sent to 23 laboratories in Europe, the USA and Canada. Laboratories prepared eight nanoparticle preparations in two food simulants (distilled water; 10% ethanol) and reported median particle size, Ag particle mass concentration and Ag particle number concentrations. Average repeatability and reproducibility standard deviation (sr and sR) for the median particle diameter were 1 and 14 nm, respectively. Relative precision was worse for Ag particle number concentrations (RSD r = 11%; RSD R = 78%). While further improvements of the method, especially with respect to software tools for evaluation, hardware options for shorter dwell times, calibration standards for determining nebuliser efficiency and further experience by laboratories are certainly desirable, the results of this study demonstrate the suitability of sp-ICPMS for the detection and quantification of certain kinds of nanoparticles.

  19. Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes

    International Nuclear Information System (INIS)

    Mascalchi, Patrice; Lamort, Anne Sophie; Salomé, Laurence; Dumas, Fabrice

    2012-01-01

    Highlights: ► We studied the diffusion of single CD4 receptors on living lymphocytes. ► This study reveals that CD4 receptors have either a random or confined diffusion. ► The dynamics of unconfined CD4 receptors was accelerated by a temperature raise. ► The dynamics of confined CD4 receptors was unchanged by a temperature raise. ► Our results suggest the existence of two different environments for CD4 receptors. -- Abstract: We investigated the lateral diffusion of the HIV receptor CD4 at the surface of T lymphocytes at 20 °C and 37 °C by Single Particle Tracking using Quantum Dots. We found that the receptors presented two major distinct behaviors that were not equally affected by temperature changes. About half of the receptors showed a random diffusion with a diffusion coefficient increasing upon raising the temperature. The other half of the receptors was permanently or transiently confined with unchanged dynamics on raising the temperature. These observations suggest that two distinct subpopulations of CD4 receptors with different environments are present at the surface of living T lymphocytes.

  20. Chemical Structure, Ensemble and Single-Particle Spectroscopy of Thick-Shell InP-ZnSe Quantum Dots.

    Science.gov (United States)

    Reid, Kemar R; McBride, James R; Freymeyer, Nathaniel J; Thal, Lucas B; Rosenthal, Sandra J

    2018-02-14

    Thick-shell (>5 nm) InP-ZnSe colloidal quantum dots (QDs) grown by a continuous-injection shell growth process are reported. The growth of a thick crystalline shell is attributed to the high temperature of the growth process and the relatively low lattice mismatch between the InP core and ZnSe shell. In addition to a narrow ensemble photoluminescence (PL) line-width (∼40 nm), ensemble and single-particle emission dynamics measurements indicate that blinking and Auger recombination are reduced in these heterostructures. More specifically, high single-dot ON-times (>95%) were obtained for the core-shell QDs, and measured ensemble biexciton lifetimes, τ 2x ∼ 540 ps, represent a 7-fold increase compared to InP-ZnS QDs. Further, high-resolution energy dispersive X-ray (EDX) chemical maps directly show for the first time significant incorporation of indium into the shell of the InP-ZnSe QDs. Examination of the atomic structure of the thick-shell QDs by high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) reveals structural defects in subpopulations of particles that may mitigate PL efficiencies (∼40% in ensemble), providing insight toward further synthetic refinement. These InP-ZnSe heterostructures represent progress toward fully cadmium-free QDs with superior photophysical properties important in biological labeling and other emission-based technologies.

  1. Confined Diffusion Without Fences of a G-Protein-Coupled Receptor as Revealed by Single Particle Tracking

    Science.gov (United States)

    Daumas, Frédéric; Destainville, Nicolas; Millot, Claire; Lopez, André; Dean, David; Salomé, Laurence

    2003-01-01

    Single particle tracking is a powerful tool for probing the organization and dynamics of the plasma membrane constituents. We used this technique to study the μ-opioid receptor belonging to the large family of the G-protein-coupled receptors involved with other partners in a signal transduction pathway. The specific labeling of the receptor coupled to a T7-tag at its N-terminus, stably expressed in fibroblastic cells, was achieved by colloidal gold coupled to a monoclonal anti T7-tag antibody. The lateral movements of the particles were followed by nanovideomicroscopy at 40 ms time resolution during 2 min with a spatial precision of 15 nm. The receptors were found to have either a slow or directed diffusion mode (10%) or a walking confined diffusion mode (90%) composed of a long-term random diffusion and a short-term confined diffusion, and corresponding to a diffusion confined within a domain that itself diffuses. The results indicate that the confinement is due to an effective harmonic potential generated by long-range attraction between the membrane proteins. A simple model for interacting membrane proteins diffusion is proposed that explains the variations with the domain size of the short-term and long-term diffusion coefficients. PMID:12524289

  2. Correlation of Dual Colour Single Particle Trajectories for Improved Detection and Analysis of Interactions in Living Cells

    Directory of Open Access Journals (Sweden)

    Kevin Braeckmans

    2013-08-01

    Full Text Available Interactions between objects inside living cells are often investigated by looking for colocalization between fluorescence microscopy images that are recorded in separate colours corresponding to the fluorescent label of each object. The fundamental limitation of this approach in the case of dynamic objects is that coincidental colocalization cannot be distinguished from true interaction. Instead, correlation between motion trajectories obtained by dual colour single particle tracking provides a much stronger indication of interaction. However, frequently occurring phenomena in living cells, such as immobile phases or transient interactions, can limit the correlation to small parts of the trajectories. The method presented here, developed for the detection of interaction, is based on the correlation inside a window that is scanned along the trajectories, covering different subsets of the positions. This scanning window method was validated by simulations and, as an experimental proof of concept, it was applied to the investigation of the intracellular trafficking of polymeric gene complexes by endosomes in living retinal pigment epithelium cells, which is of interest to ocular gene therapy.

  3. 3D Mapping of the SPRY2 domain of ryanodine receptor 1 by single-particle cryo-EM.

    Directory of Open Access Journals (Sweden)

    Alex Perálvarez-Marín

    Full Text Available The type 1 skeletal muscle ryanodine receptor (RyR1 is principally responsible for Ca(2+ release from the sarcoplasmic reticulum and for the subsequent muscle contraction. The RyR1 contains three SPRY domains. SPRY domains are generally known to mediate protein-protein interactions, however the location of the three SPRY domains in the 3D structure of the RyR1 is not known. Combining immunolabeling and single-particle cryo-electron microscopy we have mapped the SPRY2 domain (S1085-V1208 in the 3D structure of RyR1 using three different antibodies against the SPRY2 domain. Two obstacles for the image processing procedure; limited amount of data and signal dilution introduced by the multiple orientations of the antibody bound in the tetrameric RyR1, were overcome by modifying the 3D reconstruction scheme. This approach enabled us to ascertain that the three antibodies bind to the same region, to obtain a 3D reconstruction of RyR1 with the antibody bound, and to map SPRY2 to the periphery of the cytoplasmic domain of RyR1. We report here the first 3D localization of a SPRY2 domain in any known RyR isoform.

  4. Detection and characterisation of aluminium-containing nanoparticles in Chinese noodles by single particle ICP-MS.

    Science.gov (United States)

    Loeschner, Katrin; Correia, Manuel; López Chaves, Carlos; Rokkjær, Inge; Sloth, Jens J

    2018-01-01

    This study investigated Chinese noodles for the presence of aluminium-containing nanoparticles by using inductively coupled plasma mass spectrometry in single particle mode (spICP-MS) after enzymatic digestion by α-amylase. The aluminium concentrations in the noodle samples, determined by conventional ICP-MS without or with the use of hydrofluoric acid for digestion, were 5.4 ± 1.9 µg/g and 10.1 ± 2.2 µg/g (N = 21), respectively. Aluminium-containing nanoparticles were detected by spICP-MS in all 21 samples. Depending on the assumed particle composition, Al 2 O 3 or Al 2 O 3 ∙2SiO 2 ∙2H 2 O, the median particle diameters were either below or above 100 nm, respectively. The minimum detectable particle diameter by spICP-MS was between 54 and 83 nm. The mass recovery of aluminium in the form of particles was between 5% and 18%. The presented work reports for the first time the detection of Al-containing particles in food by spICP-MS.

  5. Projected interaction picture of field operators and memory superoperators. A master equation for the single-particle Green's function in a Liouville space

    International Nuclear Information System (INIS)

    Grinberg, H.

    1983-11-01

    The projection operator method of Zwanzig and Feshbach is used to construct the time-dependent field operators in the interaction picture. The formula developed to describe the time dependence involves time-ordered cosine and sine projected evolution (memory) superoperators, from which a master equation for the interaction-picture single-particle Green's function in a Liouville space is derived. (author)

  6. Online Coupling of Flow-Field Flow Fractionation and Single Particle Inductively Coupled Plasma-Mass Spectrometry: Characterization of Nanoparticle Surface Coating Thickness and Aggregation State

    Science.gov (United States)

    Surface coating thickness and aggregation state have strong influence on the environmental fate, transport, and toxicity of engineered nanomaterials. In this study, flow-field flow fractionation coupled on-line with single particle inductively coupled plasma-mass spectrometry i...

  7. Black carbon aerosol properties measured by a single particle soot photometer in emissions from biomass burning in the laboratory and field

    Science.gov (United States)

    G. R. McMeeking; J. W. Taylor; A. P. Sullivan; M. J. Flynn; S. K. Akagi; C. M. Carrico; J. L. Collett; E. Fortner; T. B. Onasch; S. M. Kreidenweis; R. J. Yokelson; C. Hennigan; A. L. Robinson; H. Coe

    2010-01-01

    We present SP2 observations of BC mass, size distributions and mixing state in emissions from laboratory and field biomass fires in California, USA. Biomass burning is the primary global black carbon (BC) source, but understanding of the amount emitted and its physical properties at and following emission are limited. The single particle soot photometer (SP2) uses a...

  8. Single particle ICP-MS combined with a data evaluastion tool as a routine techique for the analysis of nanoparticles in complex matrices

    NARCIS (Netherlands)

    Peters, R.J.B.; Herrera-Rivera, Z.; Undas, A.K.; Lee, van der M.K.; Marvin, H.J.P.; Bouwmeester, H.; Weigel, S.

    2015-01-01

    Detection and characterization of nanoparticles (NPs) in complex media as consumer products, food and toxicological test media is an essential part of understanding the potential benefits and risks of the application of nanoparticles. Single particle ICP-MS (spICP-MS) was studied as a screening tool

  9. Multi-Mode Elastic Peak Electron Microscopy (MM-EPEM): A new imaging technique with an ultimate in-depth resolution for surface analysis.

    Science.gov (United States)

    Aymen Mahjoub, Mohamed; Monier, Guillaume; Bideux, Luc; Gruzza, Bernard; Robert-Goumet, Christine

    2018-05-01

    A non-destructive new imaging technique called Multi-Mode Elastic Peak Electron Microscopy (MM-EPEM), hypersensitive to surface chemistry and with an in-depth resolution of one atomic monolayer was developed. This method consists on performing several MM-EPEM images containing n × n pixels associated to an intensity of the elastic backscattered electrons by varying the incident electron energy in the range 200-2000 eV. This approach allows obtaining depth sampling information of the analyzed structures. Furthermore, MM-EPEM is associated with Monte-Carlo simulations describing the electron pathway in materials in order to obtain very precise quantitative information, for instance the growth mode and the organization of ultra-thin layers (2D materials) or nanoparticules. In this work, we used this new method to study the deposition of very small amount of gold down to one monolayer. Example of 3D reconstruction is also provided. Copyright © 2018 Elsevier B.V. All rights reserved.

  10. Multi-mode acquisition (MMA): An MS/MS acquisition strategy for maximizing selectivity, specificity and sensitivity of DIA product ion spectra.

    Science.gov (United States)

    Williams, Brad J; Ciavarini, Steve J; Devlin, Curt; Cohn, Steven M; Xie, Rong; Vissers, Johannes P C; Martin, LeRoy B; Caswell, Allen; Langridge, James I; Geromanos, Scott J

    2016-08-01

    In proteomics studies, it is generally accepted that depth of coverage and dynamic range is limited in data-directed acquisitions. The serial nature of the method limits both sensitivity and the number of precursor ions that can be sampled. To that end, a number of data-independent acquisition (DIA) strategies have been introduced with these methods, for the most part, immune to the sampling issue; nevertheless, some do have other limitations with respect to sensitivity. The major limitation with DIA approaches is interference, i.e., MS/MS spectra are highly chimeric and often incapable of being identified using conventional database search engines. Utilizing each available dimension of separation prior to ion detection, we present a new multi-mode acquisition (MMA) strategy multiplexing both narrowband and wideband DIA acquisitions in a single analytical workflow. The iterative nature of the MMA workflow limits the adverse effects of interference with minimal loss in sensitivity. Qualitative identification can be performed by selected ion chromatograms or conventional database search strategies. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. A Practical and Robust Execution Time-Frame Procedure for the Multi-Mode Resource-Constrained Project Scheduling Problem with Minimal and Maximal Time Lags

    Directory of Open Access Journals (Sweden)

    Angela Hsiang-Ling Chen

    2016-09-01

    Full Text Available Modeling and optimizing organizational processes, such as the one represented by the Resource-Constrained Project Scheduling Problem (RCPSP, improve outcomes. Based on assumptions and simplification, this model tackles the allocation of resources so that organizations can continue to generate profits and reinvest in future growth. Nonetheless, despite all of the research dedicated to solving the RCPSP and its multi-mode variations, there is no standardized procedure that can guide project management practitioners in their scheduling tasks. This is mainly because many of the proposed approaches are either based on unrealistic/oversimplified scenarios or they propose solution procedures not easily applicable or even feasible in real-life situations. In this study, we solve a more true-to-life and complex model, Multimode RCPSP with minimal and maximal time lags (MRCPSP/max. The complexity of the model solved is presented, and the practicality of the proposed approach is justified depending on only information that is available for every project regardless of its industrial context. The results confirm that it is possible to determine a robust makespan and to calculate an execution time-frame with gaps lower than 11% between their lower and upper bounds. In addition, in many instances, the solved lower bound obtained was equal to the best-known optimum.

  12. Screening, separation, and evaluation of xanthine oxidase inhibitors from Paeonia lactiflora using chromatography combined with a multi-mode microplate reader.

    Science.gov (United States)

    Wang, Jing; Shi, Dongfang; Zheng, Meizhu; Ma, Bing; Cui, Jing; Liu, Chunming; Liu, Chengyu

    2017-11-01

    Natural products have become one of the most important resources for discovering novel xanthine oxidase inhibitors, which are commonly employed in the treatment of hyperuricemia and gout. However, to date, few reports exist regarding the use of monoterpene glycosides as xanthine oxidase inhibitors. Thus, we herein report the use of ultrafiltration coupled with liquid chromatography in the screening of monoterpene glycoside xanthine oxidase inhibitors from the extract of Paeonia lactiflora (P. lactiflora), and both high-performance counter-current chromatography and medium-pressure liquid chromatography were employed to separate the main constituents. Furthermore, the xanthine oxidase inhibitory activities and the mechanisms of inhibition of the isolated compounds were evaluated using a multi-mode microplate reader by Molecular Devices. As a result, three monoterpene glycosides were separated by combined high-performance counter-current chromatography and medium-pressure liquid chromatography in purities of 90.4, 98.0, and 86.3%, as determined by liquid chromatography. These three compounds were identified as albiflorin, paeoniflorin, and 1-O-β-ᴅ-glucopyranosyl-8-O-benzoylpaeonisuffrone by electrospray ionization tandem mass spectrometry, and albiflorin and paeoniflorin were screened as potential xanthine oxidase inhibitors by ultrafiltration with liquid chromatography. The evaluation results of xanthine oxidase inhibitory activity corresponded with the screening results, as only albiflorin and paeoniflorin exhibited xanthine oxidase inhibitory activity. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. Developing a new method for modifying over-allocated multi-mode resource constraint schedules in the presence of preemptive resources

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    Aidin Delgoshaei

    2016-09-01

    Full Text Available The issue of resource over-allocating is a big concern for project engineers in the process of scheduling project activities. Resource over-allocating is frequently seen after initial scheduling of a project in practice and causes significant amount of efforts to modify the initial schedules. In this research, a new method is developed for modifying over-allocated schedules in a multi-mode resource constrained project scheduling problems (MRCPSPs with positive cash flows (MRCPSP-PCF. The aim is to maximize profit of the MRCPSPs or logically minimizing costs. The proposed method can be used as a macro in Microsoft Office Project® Software to modify resource over-allocated days after scheduling a project. This research considers progress payment method and preemptive resources. The proposed approach maximizes profit by scheduling activities through the resource calendar respecting to the available level of preemptive resources and activity numbers. To examine the performance of the proposed method a number of experiments derived from the literature are solved. The results are then compared with the circumstances where resource constraints are relaxed. The outcomes show that in all studied cases, the proposed algorithm can provide modified schedules with no over-allocated days. Afterward the method is applied to modify a manufacturing project in practice.

  14. Detection and Quantification of Silver Nanoparticles at Environmentally Relevant Concentrations Using Asymmetric Flow Field??Flow Fractionation Online with Single Particle Inductively Coupled Plasma Mass Spectrometry

    Science.gov (United States)

    The presence of silver nanoparticles (AgNPs) in aquatic environments could potentially cause adverse impacts on ecosystems and human health. However, current understanding of the environmental fate and transport of AgNPs is still limited because their properties in complex environmental samples cannot be accurately determined. In this study, the feasibility of using asymmetric flow field-flow fractionation (AF4) connected online with single particle inductively coupled plasma mass spectrometry (spICPMS) to detect and quantify AgNPs at environmentally relevant concentrations was investigated. The AF4 channel had a thickness of 350 00b5m and its accumulation wall was a 10 kDa regenerated cellulose membrane. A 0.02 % FL-70 surfactant solution was used as an AF4 carrier. With 1.2 mL/min AF4 cross flow rate, 1.5 mL/min AF4 channel flow rate, and 5 ms spICPMS dwell time, the AF4??spICPMS can detect and quantify 40 ?? 80 nm AgNPs, as well as Ag-SiO2 nanoparticles (51.0 nm diameter Ag core and 21.6 nm SiO2 shell), with good recovery within 30 min. This system was not only effective in differentiating and quantifying different types of AgNPs with similar hydrodynamic diameters, such as in mixtures containing Ag-SiO2 core-shell nanoparticles and 40 ?? 80 nm AgNPs, but also suitable for differentiating between 40 nm AgNPs and elevated dissolved Ag content. The study results indicate that AF4??spICPMS is capable of detecting and quantifying AgNPs and other engineered

  15. 4-D single particle tracking of synthetic and proteinaceous microspheres reveals preferential movement of nuclear particles along chromatin - poor tracks.

    Science.gov (United States)

    Bacher, Christian P; Reichenzeller, Michaela; Athale, Chaitanya; Herrmann, Harald; Eils, Roland

    2004-11-23

    The dynamics of nuclear organization, nuclear bodies and RNPs in particular has been the focus of many studies. To understand their function, knowledge of their spatial nuclear position and temporal translocation is essential. Typically, such studies generate a wealth of data that require novel methods in image analysis and computational tools to quantitatively track particle movement on the background of moving cells and shape changing nuclei. We developed a novel 4-D image processing platform (TIKAL) for the work with laser scanning and wide field microscopes. TIKAL provides a registration software for correcting global movements and local deformations of cells as well as 2-D and 3-D tracking software. With this new tool, we studied the dynamics of two different types of nuclear particles, namely nuclear bodies made from GFP-NLS-vimentin and microinjected 0.1 mum - wide polystyrene beads, by live cell time-lapse microscopy combined with single particle tracking and mobility analysis. We now provide a tool for the automatic 3-D analysis of particle movement in parallel with the acquisition of chromatin density data. Kinetic analysis revealed 4 modes of movement: confined obstructed, normal diffusion and directed motion. Particle tracking on the background of stained chromatin revealed that particle movement is directly related to local reorganization of chromatin. Further a direct comparison of particle movement in the nucleoplasm and the cytoplasm exhibited an entirely different kinetic behaviour of vimentin particles in both compartments. The kinetics of nuclear particles were slightly affected by depletion of ATP and significantly disturbed by disruption of actin and microtubule networks. Moreover, the hydration state of the nucleus had a strong impact on the mobility of nuclear bodies since both normal diffusion and directed motion were entirely abolished when cells were challenged with 0.6 M sorbitol. This effect correlated with the compaction of chromatin

  16. Thon rings from amorphous ice and implications of beam-induced Brownian motion in single particle electron cryo-microscopy.

    Science.gov (United States)

    McMullan, G; Vinothkumar, K R; Henderson, R

    2015-11-01

    We have recorded dose-fractionated electron cryo-microscope images of thin films of pure flash-frozen amorphous ice and pre-irradiated amorphous carbon on a Falcon II direct electron detector using 300 keV electrons. We observe Thon rings [1] in both the power spectrum of the summed frames and the sum of power spectra from the individual frames. The Thon rings from amorphous carbon images are always more visible in the power spectrum of the summed frames whereas those of amorphous ice are more visible in the sum of power spectra from the individual frames. This difference indicates that while pre-irradiated carbon behaves like a solid during the exposure, amorphous ice behaves like a fluid with the individual water molecules undergoing beam-induced motion. Using the measured variation in the power spectra amplitude with number of electrons per image we deduce that water molecules are randomly displaced by a mean squared distance of ∼1.1 Å(2) for every incident 300 keV e(-)/Å(2). The induced motion leads to an optimal exposure with 300 keV electrons of 4.0 e(-)/Å(2) per image with which to observe Thon rings centred around the strong 3.7 Å scattering peak from amorphous ice. The beam-induced movement of the water molecules generates pseudo-Brownian motion of embedded macromolecules. The resulting blurring of single particle images contributes an additional term, on top of that from radiation damage, to the minimum achievable B-factor for macromolecular structure determination. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

  17. Post hoc interlaboratory comparison of single particle ICP-MS size measurements of NIST gold nanoparticle reference materials.

    Science.gov (United States)

    Montoro Bustos, Antonio R; Petersen, Elijah J; Possolo, Antonio; Winchester, Michael R

    2015-09-01

    Single particle inductively coupled plasma-mass spectrometry (spICP-MS) is an emerging technique that enables simultaneous measurement of nanoparticle size and number quantification of metal-containing nanoparticles at realistic environmental exposure concentrations. Such measurements are needed to understand the potential environmental and human health risks of nanoparticles. Before spICP-MS can be considered a mature methodology, additional work is needed to standardize this technique including an assessment of the reliability and variability of size distribution measurements and the transferability of the technique among laboratories. This paper presents the first post hoc interlaboratory comparison study of the spICP-MS technique. Measurement results provided by six expert laboratories for two National Institute of Standards and Technology (NIST) gold nanoparticle reference materials (RM 8012 and RM 8013) were employed. The general agreement in particle size between spICP-MS measurements and measurements by six reference techniques demonstrates the reliability of spICP-MS and validates its sizing capability. However, the precision of the spICP-MS measurement was better for the larger 60 nm gold nanoparticles and evaluation of spICP-MS precision indicates substantial variability among laboratories, with lower variability between operators within laboratories. Global particle number concentration and Au mass concentration recovery were quantitative for RM 8013 but significantly lower and with a greater variability for RM 8012. Statistical analysis did not suggest an optimal dwell time, because this parameter did not significantly affect either the measured mean particle size or the ability to count nanoparticles. Finally, the spICP-MS data were often best fit with several single non-Gaussian distributions or mixtures of Gaussian distributions, rather than the more frequently used normal or log-normal distributions.

  18. Detection of Diffusion Heterogeneity in Single Particle Tracking Trajectories Using a Hidden Markov Model with Measurement Noise Propagation

    Science.gov (United States)

    Slator, Paddy J.; Cairo, Christopher W.; Burroughs, Nigel J.

    2015-01-01

    We develop a Bayesian analysis framework to detect heterogeneity in the diffusive behaviour of single particle trajectories on cells, implementing model selection to classify trajectories as either consistent with Brownian motion or with a two-state (diffusion coefficient) switching model. The incorporation of localisation accuracy is essential, as otherwise false detection of switching within a trajectory was observed and diffusion coefficient estimates were inflated. Since our analysis is on a single trajectory basis, we are able to examine heterogeneity between trajectories in a quantitative manner. Applying our method to the lymphocyte function-associated antigen 1 (LFA-1) receptor tagged with latex beads (4 s trajectories at 1000 frames s−1), both intra- and inter-trajectory heterogeneity were detected; 12–26% of trajectories display clear switching between diffusive states dependent on condition, whilst the inter-trajectory variability is highly structured with the diffusion coefficients being related by D 1 = 0.68D 0 − 1.5 × 104 nm2 s−1, suggestive that on these time scales we are detecting switching due to a single process. Further, the inter-trajectory variability of the diffusion coefficient estimates (1.6 × 102 − 2.6 × 105 nm2 s−1) is very much larger than the measurement uncertainty within trajectories, suggesting that LFA-1 aggregation and cytoskeletal interactions are significantly affecting mobility, whilst the timescales of these processes are distinctly different giving rise to inter- and intra-trajectory variability. There is also an ‘immobile’ state (defined as D models within membranes incorporating aggregation, binding to the cytoskeleton, or traversing membrane microdomains. PMID:26473352

  19. Dust optical properties in antarctic ice cores: application of the Single Particle Extinction and Scattering (SPES) method

    Science.gov (United States)

    Potenza, Marco; Villa, Stefano; Sanvito, Tiziano; Albani, Samuel; Delmonte, Barbara; Maggi, Valter

    2015-04-01

    From the point of view of light scattering each particle is characterized by several parameters, the size being by far the most important in determining the amount of radiated power. Nevertheless, composition, internal structure, shape do slightly affect the way light is scattered, and in turn also prevent the possibility to extract the correct size. Recovering the whole information is of paramount difficulty, if not impossibile for single particles. A trade off can be obtained by introducing the optical thickness, i.e. the product of the size and the refractive index, which determines the optical properties. Here we focus at studying the optical thickness of dust particles from the EPICA Dome C ice core. We provide for the first time a direct measurement of dust optical parameters that is the most direct information needed by climate models, and highlight important differences among samples. The SPES method is named after its capability to access both the extinction cross section and the forward scattered field amplitude for each particle. This method is well working with extremely dilute suspensions, such as Antarctic ice core samples. The SPES method is based upon combined and simultaneous measurements of the power reduction of a laser beam in presence of the particle (extinction by definition) and the interference between the intense transmitted beam and the much fainter forward scattered wave (scattering). In such a way it is possible to access both the amplitude and phase of the scattered wave, which means both the real and imaginary parts of the complex field amplitude. This makes the difference with traditional approaches. We show some preliminary results from glacial and interglacial samples from the EPICA ice core and suggest a method to extract information which is important for the light scattering properties of the ensemble of dust particles contained in each sample.

  20. Single-particle Analyses of Compositions, Morphology, and Viscosity of Aerosol Particles Collected During GoAmazon2014

    Science.gov (United States)

    Adachi, K.; Gong, Z.; Bateman, A. P.; Martin, S. T.; Cirino, G. G.; Artaxo, P.; Sedlacek, A. J., III; Buseck, P. R.

    2014-12-01

    Single-particle analysis using transmission electron microscopy (TEM) shows composition and morphology of individual aerosol particles collected during the GoAmazon2014 campaign. These TEM results indicate aerosol types and mixing states, both of which are important for evaluating particle optical properties and cloud condensation nuclei activity. The samples were collected at the T3 site, which is located in the Amazon forest with influences from the urban pollution plume from Manaus. Samples were also collected from the T0 site, which is in the middle of the jungle with minimal to no influences of anthropogenic sources. The aerosol particles mainly originated from 1) anthropogenic pollution (e.g., nanosphere soot, sulfate), 2) biogenic emissions (e.g., primary biogenic particles, organic aerosols), and 3) long-range transport (e.g., sea salts). We found that the biogenic organic aerosol particles contain homogeneously distributed potassium. Particle viscosity is important for evaluating gas-particle interactions and atmospheric chemistry for the particles. Viscosity can be estimated from the rebounding behavior at controlled relative humidities, i.e., highly viscous particles display less rebound on a plate than low-viscosity particles. We collected 1) aerosol particles from a plate (non-rebounded), 2) those that had rebounded from the plate and were then captured onto an adjacent sampling plate, and 3) particles from ambient air using a separate impactor sampler. Preliminary results show that more than 90% of non-rebounded particles consisted of nanosphere soot with or without coatings. The coatings mostly consisted of organic matter. Although rebounded particles also contain nanosphere soot (number fraction 64-69%), they were mostly internally mixed with sulfate, organic matter, or their mixtures. TEM tilted images suggested that the rebounded particles were less deformed on the substrate, whereas the non-rebounded particles were more deformed, which could

  1. Detection and Characterization of ZnO Nanoparticles in Surface and Waste Waters Using Single Particle ICPMS.

    Science.gov (United States)

    Hadioui, Madjid; Merdzan, Vladimir; Wilkinson, Kevin J

    2015-05-19

    The increasing production of ZnO nanoparticles (nZnO) makes their analysis and characterization extremely important from an ecological risk perspective, especially at the low concentrations at which they are expected to be found in natural waters. Single particle ICPMS (SP-ICPMS) is one of the few techniques available to detect and characterize nanoparticles at environmentally relevant concentrations. Unfortunately, at the very low particle concentrations where SP-ICPMS is performed, significant dissolution of the nZnO generally increases background levels of dissolved Zn to the point where measurements are not generally possible. By hyphenating SP-ICPMS with an ion-exchange resin, it was possible to characterize and quantify nZnO in order to gain insight into the nature of the nZnO in natural waters. Spiked and unspiked water samples were analyzed using a SP-ICPMS that was coupled to a column containing a strong metal binding resin (Chelex 100). In addition to the detection of ZnO nanoparticles and the determination of a size distribution in natural waters, it was possible to partition the dissolved Zn among free and/or labile and strongly bound Zn fractions. In two natural waters, a high proportion (ca. 93-100%) of dissolved Zn was measured, and the residual ZnO particles were mainly composed of small agglomerates (average sizes ranging from 133.6 to 172.4 nm in the surface water and from 167.6 to 216.4 nm in the wastewater effluent). Small numbers of small nanoparticles were also detected in nonspiked waters.

  2. Mixing state of particles with secondary species by single particle aerosol mass spectrometer in an atmospheric pollution event

    Science.gov (United States)

    Xu, Lingling; Chen, Jinsheng

    2016-04-01

    Single particle aerosol mass spectrometer (SPAMS) was used to characterize size distribution, chemical composition, and mixing state of particles in an atmospheric pollution event during 20 Oct. - 5 Nov., 2015 in Xiamen, Southeast China. A total of 533,012 particle mass spectra were obtained and clustered into six groups, comprising of industry metal (4.5%), dust particles (2.6%), carbonaceous species (70.7%), K-Rich particles (20.7%), seasalt (0.6%) and other particles (0.9%). Carbonaceous species were further divided into EC (70.6%), OC (28.5%), and mixed ECOC (0.9%). There were 61.7%, 58.3%, 4.0%, and 14.6% of particles internally mixed with sulfate, nitrate, ammonium and C2H3O, respectively, indicating that these particles had undergone significant aging processing. Sulfate was preferentially mixed with carbonaceous particles, while nitrate tended to mix with metal-containing and dust particles. Compared to clear days, the fractions of EC-, metal- and dust particles remarkably increased, while the fraction of OC-containing particles decreased in pollution days. The mixing state of particles, excepted for OC-containing particles with secondary species was much stronger in pollution days than that in clear days, which revealed the significant influence of secondary particles in atmospheric pollution. The different activity of OC-containing particles might be related to their much smaller aerodynamic diameter. These results could improve our understanding of aerosol characteristics and could be helpful to further investigate the atmospheric process of particles.

  3. Single Particle-Inductively Coupled Plasma Mass Spectroscopy Analysis of Metallic Nanoparticles in Environmental Samples with Large Dissolved Analyte Fractions.

    Science.gov (United States)

    Schwertfeger, D M; Velicogna, Jessica R; Jesmer, Alexander H; Scroggins, Richard P; Princz, Juliska I

    2016-10-18

    There is an increasing interest to use single particle-inductively coupled plasma mass spectroscopy (SP-ICPMS) to help quantify exposure to engineered nanoparticles, and their transformation products, released into the environment. Hindering the use of this analytical technique for environmental samples is the presence of high levels of dissolved analyte which impedes resolution of the particle signal from the dissolved. While sample dilution is often necessary to achieve the low analyte concentrations necessary for SP-ICPMS analysis, and to reduce the occurrence of matrix effects on the analyte signal, it is used here to also reduce the dissolved signal relative to the particulate, while maintaining a matrix chemistry that promotes particle stability. We propose a simple, systematic dilution series approach where by the first dilution is used to quantify the dissolved analyte, the second is used to optimize the particle signal, and the third is used as an analytical quality control. Using simple suspensions of well characterized Au and Ag nanoparticles spiked with the dissolved analyte form, as well as suspensions of complex environmental media (i.e., extracts from soils previously contaminated with engineered silver nanoparticles), we show how this dilution series technique improves resolution of the particle signal which in turn improves the accuracy of particle counts, quantification of particulate mass and determination of particle size. The technique proposed here is meant to offer a systematic and reproducible approach to the SP-ICPMS analysis of environmental samples and improve the quality and consistency of data generated from this relatively new analytical tool.

  4. Real time analysis of lead-containing atmospheric particles in Beijing during springtime by single particle aerosol mass spectrometry.

    Science.gov (United States)

    Ma, Li; Li, Mei; Huang, Zhengxu; Li, Lei; Gao, Wei; Nian, Huiqing; Zou, Lilin; Fu, Zhong; Gao, Jian; Chai, Fahe; Zhou, Zhen

    2016-07-01

    Using a single particle aerosol mass spectrometer (SPAMS), the chemical composition and size distributions of lead (Pb)-containing particles with diameter from 0.1 μm to 2.0 μm in Beijing were analyzed in the spring of 2011 during clear, hazy, and dusty days. Based on mass spectral features of particles, cluster analysis was applied to Pb-containing particles, and six major classes were acquired consisting of K-rich, carboneous, Fe-rich, dust, Pb-rich, and Cl-rich particles. Pb-containing particles accounted for 4.2-5.3%, 21.8-22.7%, and 3.2% of total particle number during clear, hazy and dusty days, respectively. K-rich particles are a major contribution to Pb-containing particles, varying from 30.8% to 82.1% of total number of Pb-containing particles, lowest during dusty days and highest during hazy days. The results reflect that the chemical composition and amount of Pb-containing particles has been affected by meteorological conditions as well as the emissions of natural and anthropogenic sources. K-rich particles and carbonaceous particles could be mainly assigned to the emissions of coal combustion. Other classes of Pb-containing particles may be associated with metallurgical processes, coal combustion, dust, and waste incineration etc. In addition, Pb-containing particles during dusty days were first time studied by SPAMS. This method could provide a powerful tool for monitoring and controlling of Pb pollution in real time. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. Dynamic micro-organization of P2X7 receptors revealed by PALM based single particle tracking

    Directory of Open Access Journals (Sweden)

    Amulya Nidhi Shrivastava

    2013-11-01

    Full Text Available Adenosine triphosphate (ATP-gated P2X7 receptors (P2X7Rs are members of the purinergic receptor family that are expressed in several cell types including neurons. A high concentration of ATP is required for the channel opening of P2X7Rs compared to other members of this receptor family. Recent work suggests that ATP binding to members of the P2X receptor family determines the diffusion and localization of these receptors on the plasma membrane of neurons. Here, we employed single particle tracking photoactivated localization microscopy (sptPALM to study the diffusion and ATP-dependence of rat P2X7Rs. Dendra2-tagged P2X7Rs were transfected in hippocampal neurons and imaged on proximal dendrites. Our results suggest the presence of two populations of P2X7Rs within the extra-synaptic membrane: a population composed of rapidly diffusing receptors and one stabilized within nanoclusters (~100 nm diameter. P2X7R trajectories were rarely observed at synaptic sites. P2X7R mutations in the ATP-binding site (K64A or the conserved phosphorylation site (K17A resulted in faster- and slower-diffusing receptors, respectively. Furthermore, ATP differentially accelerated wild type and K17A-mutant receptors but not K64A-mutant receptors. Our results indicate that receptor conformation plays a critical role in regulating ATP-mediated changes in P2X7R diffusion and micro-organization.

  6. Quantitative characterization of TiO2 nanoparticle release from textiles by conventional and single particle ICP-MS

    Science.gov (United States)

    Mackevica, Aiga; Olsson, Mikael Emil; Hansen, Steffen Foss

    2018-01-01

    TiO2 is ubiquitously present in a wide range of everyday items, both as an intentionally incorporated additive and naturally occurring constituent. It can be found in a wide range of consumer products, including personal care products, food contact materials, and textiles. Normal use of these products may lead to consumer and/or environmental exposure to TiO2, possibly in form of nanoparticles. The aim of this study is to perform a leaching test and apply state-of-the-art methods to investigate nano-TiO2 and total Ti release from five types of commercially available conventional textiles: table placemats, wet wipes, microfiber cloths, and two types of baby bodysuits, with Ti contents ranging from 2.63 to 1448 μg/g. Released particle analysis was performed using conventional and single particle inductively coupled plasma mass spectrometry (ICP-MS and spICP-MS), in conjunction with transmission electron microscopy (TEM), to measure total and particulate TiO2 release by mass and particle number, as well as size distribution. Less than 1% of the initial Ti content was released over 24 h of leaching, with the highest releases reaching 3.13 μg/g. The fraction of nano-TiO2 released varied among fabric types and represented 0-80% of total TiO2 release. Particle mode sizes were 50-75 nm, and TEM imaging revealed particles in sizes of 80-200 nm. This study highlights the importance of using a multi-method approach to obtain quantitative release data that is able to provide an indication regarding particle number, size distribution, and mass concentration, all of which can help in understanding the fate and exposure of nanoparticles.

  7. Insight into the in-cloud formation of oxalate based on in situ measurement by single particle mass spectrometry

    Science.gov (United States)

    Zhang, Guohua; Lin, Qinhao; Peng, Long; Yang, Yuxiang; Fu, Yuzhen; Bi, Xinhui; Li, Mei; Chen, Duohong; Chen, Jianxin; Cai, Zhang; Wang, Xinming; Peng, Ping'an; Sheng, Guoying; Zhou, Zhen

    2017-11-01

    While ground-based works suggest the significance of in-cloud production (or aqueous formation) to oxalate, direct evidence is rare. With the in situ measurements performed at a remote mountain site (1690 m above sea level) in southern China, we first reported the size-resolved mixing state of oxalate in the cloud droplet residual (cloud RES), the cloud interstitial (cloud INT), and ambient (cloud-free) particles by single particle mass spectrometry. The results support the growing evidence that in-cloud aqueous reactions promote the formation of oxalate, with ˜ 15 % of the cloud RES and cloud INT particles containing oxalate in contrast to only ˜ 5 % of the cloud-free particles. Furthermore, individual particle analysis provides unique insight into the formation of oxalate during in-cloud processing. Oxalate was predominantly (> 70 % in number) internally mixed with the aged biomass-burning particles, highlighting the impact of biomass burning on the formation of oxalate. In contrast, oxalate was underrepresented in aged elemental carbon particles, although they represented the largest fraction of the detected particles. It can be interpreted by the individual particle mixing state that the aged biomass-burning particles contained an abundance of organic components serving as precursors for oxalate. Through the analysis of the relationship between oxalate and organic acids (-45[HCO2]-, -59[CH3CO2]-, -71[C2H3CO2]-, -73[C2HO3]-), the results show that in-cloud aqueous reactions dramatically improved the conversion of organic acids to oxalate. The abundance of glyoxylate associated with the aged biomass-burning particles is a controlling factor for the in-cloud production of oxalate. Since only limited information on oxalate is available in the free troposphere, the results also provide an important reference for future understanding of the abundance, evolution, and climate impacts of oxalate.

  8. Convergence of lateral dynamic measurements in the plasma membrane of live cells from single particle tracking and STED-FCS

    Science.gov (United States)

    Lagerholm, B. Christoffer; Andrade, Débora M.; Clausen, Mathias P.; Eggeling, Christian

    2017-02-01

    Fluorescence correlation spectroscopy (FCS) in combination with the super-resolution imaging method STED (STED-FCS), and single-particle tracking (SPT) are able to directly probe the lateral dynamics of lipids and proteins in the plasma membrane of live cells at spatial scales much below the diffraction limit of conventional microscopy. However, a major disparity in interpretation of data from SPT and STED-FCS remains, namely the proposed existence of a very fast (unhindered) lateral diffusion coefficient, ⩾5 µm2 s-1, in the plasma membrane of live cells at very short length scales, ≈⩽ 100 nm, and time scales, ≈1-10 ms. This fast diffusion coefficient has been advocated in several high-speed SPT studies, for lipids and membrane proteins alike, but the equivalent has not been detected in STED-FCS measurements. Resolving this ambiguity is important because the assessment of membrane dynamics currently relies heavily on SPT for the determination of heterogeneous diffusion. A possible systematic error in this approach would thus have vast implications in this field. To address this, we have re-visited the analysis procedure for SPT data with an emphasis on the measurement errors and the effect that these errors have on the measurement outputs. We subsequently demonstrate that STED-FCS and SPT data, following careful consideration of the experimental errors of the SPT data, converge to a common interpretation which for the case of a diffusing phospholipid analogue in the plasma membrane of live mouse embryo fibroblasts results in an unhindered, intra-compartment, diffusion coefficient of  ≈0.7-1.0 µm2 s-1, and a compartment size of about 100-150 nm.

  9. Personal Exposure to PM2.5 in the Megacity of Mexico: A Multi-Mode Transport Study

    Directory of Open Access Journals (Sweden)

    Iván Y. Hernández-Paniagua

    2018-02-01

    Full Text Available Recurrent personal exposure to ambient PM2.5 is associated with adverse human health effects, in particular on the respiratory and cardiovascular systems. Here, we present an assessment of personal exposure and inhalation of PM2.5 for five modes of transport (walking, cycling, public bus (trolleybus and diesel bus, conventional car (CC and hybrid-electric car (HEC and two routes of similar distance, along a major road in the Mexico City metropolitan area (MCMA. Arithmetic average exposure concentrations ranged from 16.5 ± 6.5 µg m−3 for walking to 81.7 ± 9.1 µg m−3 for cycling (henceforth shown as average ±1 SD, with no significant differences with geometric averages. The maximum exposure concentration of 110.9 µg m−3 was observed for the conventional car. The highest exposure concentrations depended on route and the mode of transport, being observed for cycling and walking. The PM2.5 measurements showed large spatial heterogeneity in the exposure levels for walking and cycling, while public buses and private transport showed less spatial heterogeneity. The greatest peaks in PM2.5 coincided with 4-way intersections for all modes of transport, being positively influenced by traffic density. The mass of PM2.5 inhaled depended mostly on the mode of transport, and ranged between 1.0 ± 0.3 and 30.1 ± 14.2 µg km−1 for the HEC and bicycle, respectively. Local area PM2.5 increments identified as ‘residuals’ after subtraction of data recorded at the closest fixed monitoring site from exposure concentrations along the studied road suggested that inhalation for bicycle and diesel buses is strongly influenced by vehicular emissions. Residuals estimated for the trolleybus, CC and HEC confirmed a lower inhalation than for the other modes of transport evaluated due to protection by the cabin.

  10. Continuous quantum mechanics of single particles in closed and quasi-closed systems: Pt. III and IV

    Energy Technology Data Exchange (ETDEWEB)

    Brieger, M. [DLR Deutsches Zentrum fuer Luft- und Raumfahrt e.V., Stuttgart (Germany). Inst. fuer Technische Physik

    2005-07-01

    The rigorous solution to Schroedinger's nonrelativistic time-dependent equation of a single electron's spin-orbit or magnetic hyperfine interaction reveals the full dynamics of angular momentum coupling especially in the presence of an external, arbitrarily oriented magnetic field. Besides of making a description of major dynamical properties possible in simple classical terms, it also sheds new light on the seeming quantization of states: They turn out to be exceptional states of dynamical balance, which in the field-free case are only made possible by the respective pairs of Clebsch-Gordan coefficients. Moreover, the results not only show that integer and half-integer quantum numbers of the total angular momentum only apply to this special case but that in the general case they are also able to obtain the well-known splitting pattern of the stationary energy levels in the field as produced by separately identifiable, field-dependent contributions from the spin-orbit or the magnetic hyperfine interactions on the one hand, and the magnetic dipole interaction with external magnetic fields on the other in a way not accessible to the Breit-Rabi formula. The results further demonstrate that the total magnetic moment responds quite sensitively to even weak field strengths, i.e., with substantial changes although the linear Zeeman effect suggests their seeming constancy in low fields also for levels with vertical stroke M{sub J} vertical stroke < l+1/2. Although Schroedinger's equation is equivalent to an energy representation this detailed description of the behavior of the total magnetic moment is made possible by the fortunate fact that the coupling magnetic moments enter the Hamiltonian linearly. This circumstance can be exploited to describe in full detail how the total magnetic moment behaves in an arbitrarily oriented magnetic field. It is found that its motion in this environment consists of a basic Larmor precession about the field direction superimposed on which is an oscillation of the coupling angle. The characteristic frequencies of the total magnetic moment's longitudinal and transverse motions relative to the field direction are in compliance with Bohr's frequency condition and the selection rules for magnetic dipole transitions in exactly the way their frequencies and polarizations are also observed experimentally. (orig.)

  11. Studies of propane flame soot acting as heterogeneous ice nuclei in conjunction with single particle soot photometer measurements

    Directory of Open Access Journals (Sweden)

    I. Crawford

    2011-09-01

    Full Text Available The ice nucleation efficiency of propane flame soot particles with and without a sulphuric acid coating was investigated using the aerosol and cloud chamber facility AIDA (Aerosol Interaction and Dynamics in the Atmosphere. The test soot for cloud formation simulations was produced using a propane flame Combustion Aerosol Standard generator (CAST, Jing-CAST Technologies. The organic carbon content (OC of the test soot was altered in a reproducible fashion by changing the fuel/air mixture of the generator. The soot content of ice nuclei was subsequently investigated using a combination of a pumped counterflow virtual impactor (PCVI to separate and evaporate the ice crystals, and a DMT single particle soot photometer (SP2 to examine the mixing state of the BC containing ice residuals.

    Ice nucleation was found to be most efficient for uncoated soot of low organic carbon content (~5 % organic carbon content where deposition freezing occurred at an ice saturation ratio Sice ~ 1.22 at a temperature T = 226.6 K with 25 % of the test soot becoming active as ice nuclei. Propane flame soot of higher organic carbon content (~30 % and ~70 % organic carbon content showed significantly lower ice nucleation efficiency (an activated fraction of the order of a few percent in the experiments than the low organic carbon content soot, with water saturation being required for freezing to occur. Ice nucleation occurred over the range Sice = 1.22–1.70, and T = 223.2–226.6 K. Analysis of the SP2 data showed that the 5 % organic carbon content soot had an undetectable OC coating whereas the 30 % organic carbon content soot had a thicker or less volatile OC coating.

    The application of a sulphuric acid coating to the flame soot shifted the threshold of the onset of freezing towards that of the homogeneous freezing of sulphuric acid; for the minimum OC flame soot this inhibited nucleation since the

  12. Quantification and size characterisation of silver nanoparticles in environmental aqueous samples and consumer products by single particle-ICPMS.

    Science.gov (United States)

    Aznar, Ramón; Barahona, Francisco; Geiss, Otmar; Ponti, Jessica; José Luis, Tadeo; Barrero-Moreno, Josefa

    2017-12-01

    Single particle-inductively coupled plasma mass spectrometry (SP-ICPMS) is a promising technique able to generate the number based-particle size distribution (PSD) of nanoparticles (NPs) in aqueous suspensions. However, SP-ICPMS analysis is not consolidated as routine-technique yet and is not typically applied to real test samples with unknown composition. This work presents a methodology to detect, quantify and characterise the number-based PSD of Ag-NPs in different environmental aqueous samples (drinking and lake waters), aqueous samples derived from migration tests and consumer products using SP-ICPMS. The procedure is built from a pragmatic view and involves the analysis of serial dilutions of the original sample until no variation in the measured size values is observed while keeping particle counts proportional to the dilution applied. After evaluation of the analytical figures of merit, the SP-ICPMS method exhibited excellent linearity (r 2 >0.999) in the range (1-25) × 10 4 particlesmL -1 for 30, 50 and 80nm nominal size Ag-NPs standards. The precision in terms of repeatability was studied according to the RSDs of the measured size and particle number concentration values and a t-test (p = 95%) at the two intermediate concentration levels was applied to determine the bias of SP-ICPMS size values compared to reference values. The method showed good repeatability and an overall acceptable bias in the studied concentration range. The experimental minimum detectable size for Ag-NPs ranged between 12 and 15nm. Additionally, results derived from direct SP-ICPMS analysis were compared to the results conducted for fractions collected by asymmetric flow-field flow fractionation and supernatant fractions after centrifugal filtration. The method has been successfully applied to determine the presence of Ag-NPs in: lake water; tap water; tap water filtered by a filter jar; seven different liquid silver-based consumer products; and migration solutions (pure water and

  13. New approaches for the chemical and physical characterization of aerosols using a single particle mass spectrometry based technique

    Science.gov (United States)

    Spencer, Matthew Todd

    burning and appeared to be internally mixed with sulfate which suggests it was cloud processed during transport. Lastly, noble metal nanoparticles are explored as potential matrices for visible wavelength single particle matrix assisted laser desorption/ionization mass spectrometry (VIS-MALDI). This work demonstrates that noble metal nanoparticle matrices can be used for VIS-MALDI analysis.

  14. Insight into the in-cloud formation of oxalate based on in situ measurement by single particle mass spectrometry

    Directory of Open Access Journals (Sweden)

    G. Zhang

    2017-11-01

    Full Text Available While ground-based works suggest the significance of in-cloud production (or aqueous formation to oxalate, direct evidence is rare. With the in situ measurements performed at a remote mountain site (1690 m above sea level in southern China, we first reported the size-resolved mixing state of oxalate in the cloud droplet residual (cloud RES, the cloud interstitial (cloud INT, and ambient (cloud-free particles by single particle mass spectrometry. The results support the growing evidence that in-cloud aqueous reactions promote the formation of oxalate, with  ∼  15 % of the cloud RES and cloud INT particles containing oxalate in contrast to only  ∼  5 % of the cloud-free particles. Furthermore, individual particle analysis provides unique insight into the formation of oxalate during in-cloud processing. Oxalate was predominantly (> 70 % in number internally mixed with the aged biomass-burning particles, highlighting the impact of biomass burning on the formation of oxalate. In contrast, oxalate was underrepresented in aged elemental carbon particles, although they represented the largest fraction of the detected particles. It can be interpreted by the individual particle mixing state that the aged biomass-burning particles contained an abundance of organic components serving as precursors for oxalate. Through the analysis of the relationship between oxalate and organic acids (−45[HCO2]−, −59[CH3CO2]−, −71[C2H3CO2]−, −73[C2HO3]−, the results show that in-cloud aqueous reactions dramatically improved the conversion of organic acids to oxalate. The abundance of glyoxylate associated with the aged biomass-burning particles is a controlling factor for the in-cloud production of oxalate. Since only limited information on oxalate is available in the free troposphere, the results also provide an important reference for future understanding of the abundance, evolution, and climate impacts of oxalate.

  15. Crystallographic and single-particle analyses of native- and nucleotide-bound forms of the cystic fibrosis transmembrane conductance regulator (CFTR) protein.

    Science.gov (United States)

    Awayn, N H; Rosenberg, M F; Kamis, A B; Aleksandrov, L A; Riordan, J R; Ford, R C

    2005-11-01

    Cystic fibrosis, one of the major human inherited diseases, is caused by defects in the CFTR (cystic fibrosis transmembrane conductance regulator), a cell-membrane protein. CFTR acts as a chloride channel which can be opened by ATP. Low-resolution structural studies of purified recombinant human CFTR are described in the present paper. Localization of the C-terminal decahistidine tag in CFTR was achieved by Ni2+-nitriloacetate nanogold labelling, followed by electron microscopy and single-particle analysis. The presence of the gold label appears to improve the single-particle-alignment procedure. Projection structures of CFTR from two-dimensional crystals analysed by electron crystallography displayed two alternative conformational states in the presence of nucleotide and nanogold, but only one form of the protein was observed in the quiescent (nucleotide-free) state.

  16. 48Ca(d,n)49Sc reaction at E/sub d/=20 MeV; proton single-particle states in 49Sc

    International Nuclear Information System (INIS)

    Iwasaki, Y.; Galonsky, A.; Weber, D.J.

    1980-01-01

    The 48 Ca(d,n) 49 Sc reaction has been studied at E/sub d/=20 MeV. Angular distributions of differential cross sections have been obtained for 14 transitions to states in 49 Sc up to an excitation energy of 7.1 MeV. A distorted-wave Born-approximation analysis has been made of the experimental data. With respect to states corresponding to the same proton single-particle orbital, relative values of derived spectroscopic factors are generally in good agreement with those obtained from ( 3 He,d) reaction data. There are remarkable differences between the results from the 48 Ca(d,n) 49 Sc reaction and the 48 Ca( 3 He,d) 49 Sc, however, regarding the dependence of the relative spectroscopic factors on proton single-particle orbitals

  17. Spectroscopy of low-lying single-particle states in $^{81}$Zn populated in the $^{80}$Zn(d,p) reaction

    CERN Multimedia

    The aim of this proposal is the study of single-particle states of $^{81}$Zn via the $^{80}$Zn(d,p) reaction in inverse kinematics. $^{81}$Zn will be produced by means of a laser-ionized, 5.5 MeV/u HIE-Isolde $^{80}$Zn beam impinging on a deuterated-polyethylene target. The protons and $\\gamma$-rays emitted in the reaction will be studied using the Miniball + T-REX setup. This experiment will constitute the first spectroscopic study of $^{81}$Zn, which is critically important to determine the energy and ordering of neutron single-particle orbits above the N=50 gap and the properties of $^{78}$Ni.

  18. Characteristics of atmospheric single particles during haze periods in a typical urban area of Beijing: A case study in October, 2014.

    Science.gov (United States)

    Liu, Lang; Wang, Yanli; Du, Shiyong; Zhang, Wenjie; Hou, Lujian; Vedal, Sverre; Han, Bin; Yang, Wen; Chen, Mindong; Bai, Zhipeng

    2016-02-01

    To investigate the composition and possible sources of particles, especially during heavy haze pollution, a single particle aerosol mass spectrometer (SPAMS) was deployed to measure the changes of single particle species and sizes during October of 2014, in Beijing. A total of 2,871,431 particles with both positive and negative spectra were collected and characterized in combination with the adaptive resonance theory neural network algorithm (ART-2a). Eight types of particles were classified: dust particles (dust, 8.1%), elemental carbon (EC, 29.0%), organic carbon (OC, 18.0%), EC and OC combined particles (ECOC, 9.5%), Na-K containing particles (NaK, 7.9%), K-containing particles (K, 21.8%), organic nitrogen and potassium containing particles (KCN, 2.3%), and metal-containing particles (metal, 3.6%). Three haze pollution events (P1, P2, P3) and one clean period (clean) were analyzed, based on the mass and number concentration of PM2.5 and the back trajectory results from the hybrid single particle Lagrangian integrated trajectory model (Hysplit-4 model). Results showed that EC, OC and K were the major components of single particles during the three haze pollution periods, which showed clearly increased ratios compared with those in the clean period. Results from the mixing state of secondary species of different types of particles showed that sulfate and nitrate were more readily mixed with carbon-containing particles during haze pollution episodes than in clean periods. Copyright © 2015. Published by Elsevier B.V.

  19. Method and apparatus for operating a powertrain system upon detecting a stuck-closed clutch

    Science.gov (United States)

    Hansen, R. Anthony

    2014-02-18

    A powertrain system includes a multi-mode transmission having a plurality of torque machines. A method for controlling the powertrain system includes identifying all presently applied clutches including commanded applied clutches and the stuck-closed clutch upon detecting one of the torque-transfer clutches is in a stuck-closed condition. A closed-loop control system is employed to control operation of the multi-mode transmission accounting for all the presently applied clutches.

  20. Study of single particle properties of nuclei in the region of the "island of inversion" by means of neutron-transfer reactions

    CERN Multimedia

    Kruecken, R; Voulot, D

    2007-01-01

    We are aiming at the investigation of single particle properties of neutron-rich nuclei in the region of the "island of inversion" where intruder states from the $\\{fp}$-shell favour deformed ground states instead of the normal spherical $\\textit{sd}$-shell states. As first experiment, we propose to study single particle states in the neutron-rich isotope $^{31}$Mg. The nucleus will be populated by a one-neutron transfer reaction with a $^{30}$Mg beam at 3 MeV/u obtained from REX-ISOLDE impinging on a CD$_{2}$ target. The $\\gamma$-rays will be detected by the MINIBALL array and the particles by a newly built set-up of segmented Si detectors with a angular coverage of nearly 4$\\pi$. Relative spectroscopic factors extracted from the cross sections will enable us to pin down the configurations of the populated states. These will be compared to recent shell model calculations involving new residual interactions. This will shed new light on the evolution of single particle structure leading to the breaking of the ...

  1. A study of the diffusion dynamics and concentration distribution of gold nanospheres (GNSs) without fluorescent labeling inside live cells using fluorescence single particle spectroscopy.

    Science.gov (United States)

    Liu, Fangchao; Dong, Chaoqing; Ren, Jicun

    2018-03-15

    Colloidal gold nanospheres (GNSs) have become important nanomaterials in biomedical applications due to their special optical properties, good chemical stability, and biocompatibility. However, measuring the diffusion coefficients or concentration distribution of GNSs within live cells accurately without any extra fluorescent labeling in situ has still not been resolved. In this work, a single particle method is developed to study the concentration distribution of folic acid-modified GNSs (FA-GNSs) internalized via folate receptors, and investigates their diffusion dynamics within live cells using single particle fluorescence correlation spectroscopy (FCS). We optimized the experimental conditions and verified the feasibility of 30 nm GNSs without extra fluorescence labeling being used for single particle detection inside live cells. Then, the FCS characterization strategy was used to measure the concentration and diffusion coefficient distributions of GNSs inside live cells and the obtained results were basically in agreement with those obtained by TEM. The results demonstrate that our strategy is characterized as an in situ, nondestructive, rapid and dynamic method for the assay of live cells, and it may be widely used in the further design of GNP-based drug delivery and therapeutics.

  2. Arctic Black Carbon Loading and Profile Using the Single-Particle Soot Photometer (SP2) Field Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    Sedlacek, Arthur J [Brookhaven National Lab. (BNL), Upton, NY (United States)

    2016-05-01

    One of the major issues confronting aerosol climate simulations of the Arctic and Antarctic cryospheres is the lack of detailed data on the vertical and spatial distribution of aerosols with which to test these models. This is due, in part, to the inherent difficulty of conducting such measurements in extreme environments. However given the pronounced sensitivity of the polar regions to radiative balance perturbations, it is incumbent upon our community to better understand and quantify these perturbations, and their unique feedbacks, so that robust model predictions of this region can be realized. One class of under-measured radiative forcing agents in the polar region is the absorbing aerosol—black carbon and brown carbon. Black carbon (BC; also referred to as light-absorbing carbon [LAC], refractory black carbon [rBC], and soot) is second only to CO2 as a positive forcing agent. Roughly 60% of BC emissions can be attributed to anthropogenic sources (fossil fuel combustion and open-pit cooking), with the remaining fraction being due to biomass burning. Brown carbon (BrC), a major component of biomass burning, collectively refers to non-BC carbonaceous aerosols that typically possess minimal light absorption at visible wavelengths but exhibit pronounced light absorption in the near-ultraviolet (UV) spectrum. Both species can be sourced locally or be remotely transported to the Arctic region and are expected to perturb the radiative balance. The work conducted in this field campaign addresses one of the more glaring deficiencies currently limiting improved quantification of the impact of BC radiative forcing in the cryosphere: the paucity of data on the vertical and spatial distributions of BC. By expanding the Gulfstream aircraft (G-1) payload for the U.S. Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) Climate Research Facility-sponsored ACME-V campaign to include the Single-Particle Soot Photometer (SP2)) and leveraging the ACME-V campaign

  3. Single particle chemical composition, state of mixing and shape of fresh and aged Saharan dust in Morocco and at Cape Verde Islands during SAMUM I and II

    Science.gov (United States)

    Kandler, Konrad; Emmel, Carmen; Ebert, Martin; Lieke, Kirsten; Müller-Ebert, Dörthe; Schütz, Lothar; Weinbruch, Stephan

    2010-05-01

    The Saharan Mineral Dust Experiment (SAMUM) is focussed to the understanding of the radiative effects of mineral dust. During the SAMUM 2006 field campaign at Tinfou, southern Morocco, chemical and mineralogical properties of fresh desert aerosol was measured. The winter campaign of Saharan Mineral Dust Experiment II in 2008 was based in Praia, Island of Santiago, Cape Verde. This second field campaign was dedicated to the investigation of transported Saharan Mineral Dust. Ground-based and airborne measurements were performed in the winter season, where mineral dust from the Western Sahara and biomass burning aerosol from the Sahel region occurred. Samples were collected with a miniature impactor system, a sedimentation trap, a free-wing impactor, and a filter sampler. Beryllium discs as well as carbon coated nickel discs, carbon foils, and nuclepore and fiber filters were used as sampling substrates. The size-resolved particle aspect ratio and the chemical composition are determined by scanning electron microscopy and energy-dispersive X-ray microanalysis of single particles. Mineralogical bulk composition is determined by X-ray diffraction analysis. In Morocco, three size regimes are identified in the aerosol: Smaller than 500 nm in diameter, the aerosol consists of sulfates and mineral dust. Larger than 500 nm up to 50 µm, mineral dust dominates, consisting mainly of silicates, and - to a lesser extent - carbonates and quartz. Larger than 50 µm, approximately half of the particles consist of quartz. Time series of the elemental composition show a moderate temporal variability of the major compounds. Calcium-dominated particles are enhanced during advection from a prominent dust source in Northern Africa (Chott El Djerid and surroundings). More detailed results are found in Kandler et al. (2009) At Praia, Cape Verde, the boundary layer aerosol consists of a superposition of mineral dust, marine aerosol and ammonium sulfate, soot, and other sulfates as well as

  4. Airborne single particle mass spectrometers (SPLAT II & miniSPLAT) and new software for data visualization and analysis in a geo-spatial context.

    Science.gov (United States)

    Zelenyuk, Alla; Imre, Dan; Wilson, Jacqueline; Zhang, Zhiyuan; Wang, Jun; Mueller, Klaus

    2015-02-01

    Understanding the effect of aerosols on climate requires knowledge of the size and chemical composition of individual aerosol particles-two fundamental properties that determine an aerosol's optical properties and ability to serve as cloud condensation or ice nuclei. Here we present our aircraft-compatible single particle mass spectrometers, SPLAT II and its new, miniaturized version, miniSPLAT that measure in-situ and in real-time the size and chemical composition of individual aerosol particles with extremely high sensitivity, temporal resolution, and sizing precision on the order of a monolayer. Although miniSPLAT's size, weight, and power consumption are significantly smaller, its performance is on par with SPLAT II. Both instruments operate in dual data acquisition mode to measure, in addition to single particle size and composition, particle number concentrations, size distributions, density, and asphericity with high temporal resolution. We also present ND-Scope, our newly developed interactive visual analytics software package. ND-Scope is designed to explore and visualize the vast amount of complex, multidimensional data acquired by our single particle mass spectrometers, along with other aerosol and cloud characterization instruments on-board aircraft. We demonstrate that ND-Scope makes it possible to visualize the relationships between different observables and to view the data in a geo-spatial context, using the interactive and fully coupled Google Earth and Parallel Coordinates displays. Here we illustrate the utility of ND-Scope to visualize the spatial distribution of atmospheric particles of different compositions, and explore the relationship between individual particle compositions and their activity as cloud condensation nuclei.

  5. Chemical composition of ambient aerosol, ice residues and cloud droplet residues in mixed-phase clouds: single particle analysis during the Cloud and Aerosol Characterization Experiment (CLACE 6

    Directory of Open Access Journals (Sweden)

    M. Kamphus

    2010-08-01

    Full Text Available Two different single particle mass spectrometers were operated in parallel at the Swiss High Alpine Research Station Jungfraujoch (JFJ, 3580 m a.s.l. during the Cloud and Aerosol Characterization Experiment (CLACE 6 in February and March 2007. During mixed phase cloud events ice crystals from 5–20 μm were separated from larger ice aggregates, non-activated, interstitial aerosol particles and supercooled droplets using an Ice-Counterflow Virtual Impactor (Ice-CVI. During one cloud period supercooled droplets were additionally sampled and analyzed by changing the Ice-CVI setup. The small ice particles and droplets were evaporated by injection into dry air inside the Ice-CVI. The resulting ice and droplet residues (IR and DR were analyzed for size and composition by the two single particle mass spectrometers: a custom-built Single Particle Laser-Ablation Time-of-Flight Mass Spectrometer (SPLAT and a commercial Aerosol Time-of-Flight Mass Spectrometer (ATOFMS, TSI Model 3800. During CLACE 6 the SPLAT instrument characterized 355 individual IR that produced a mass spectrum for at least one polarity and the ATOFMS measured 152 IR. The mass spectra were binned in classes, based on the combination of dominating substances, such as mineral dust, sulfate, potassium and elemental carbon or organic material. The derived chemical information from the ice residues is compared to the JFJ ambient aerosol that was sampled while the measurement station was out of clouds (several thousand particles analyzed by SPLAT and ATOFMS and to the composition of the residues of supercooled cloud droplets (SPLAT: 162 cloud droplet residues analyzed, ATOFMS: 1094. The measurements showed that mineral dust was strongly enhanced in the ice particle residues. Close to all of the SPLAT spectra from ice residues did contain signatures from mineral compounds, albeit connected with varying amounts of soluble compounds. Similarly, close to all of the ATOFMS IR spectra show a

  6. Experimental investigation on multidisciplinary geophysical characterization of deep underground structure using multi-scale, multi-mode seismic profiling for the evaluation of ground motion and seismic model building

    International Nuclear Information System (INIS)

    Abe, Susumu

    2014-01-01

    Recent advancements in data acquisition and velocity estimation for multi-mode, multiscale seismic exploration were explained along with the basic concept of strategic geophysical surveys for NPP siting assessment. Then, as a case study using this concept, multidisciplinary geophysical characterization results pertaining to the deep underground structure beneath the JNES Kashiwazaki Center were explained in detail. At the site, reflection/refraction surveys and magnetotelluric/gravity surveys were also conducted. It was shown that these surveys can be used complementary because at the upsurge part, where clear images cannot be obtained by reflection/refraction surveys, magnetotelluric /gravity surveys can be used to obtain clear images. (author)

  7. Dielectronic recombination experiments with tungsten ions at the test storage ring and development of a single-particle detector at the cryogenic storage ring

    International Nuclear Information System (INIS)

    Spruck, Kaija

    2015-05-01

    This work is about electron-ion collision experiments at the ion storage rings of the Max Planck Institute for Nuclear Physics in Heidelberg. Absolute recombination rate coefficients of highly-charged tungsten ions featuring an open 4-f-shell structure have been measured at the heavy-ion storage ring TSR. The resulting plasma rate coefficients have been used to probe the significance of newly developed theoretical approaches. Plasma rate coefficients of highly-charged tungsten ions are in particular interesting for the development of plasma models for nuclear fusion reactors, since tungsten is a foreseeable impurity in the fusion plasma. In the relevant temperature range, the experimental results exceed the theoretical data used so far by up to a factor of 10, showing the need for more reliable theoretical calculations. Furthermore, based on the design of the detectors which have been used in the experiments at TSR, a movable single-particle detector for electron-ion recombination studies at the cryogenic storage ring CSR has been developed and installed within the scope of this work. The device has been designed specifically to meet the requirements of the CSR regarding low ion energies and cryogenic ambient temperature conditions. In a series of experiments, the detector was carefully characterised and successfully tested for its compatibility with these requirements. The detector was part of the infrastructure used for the room-temperature commissioning of CSR (2014) and is currently operated as a single-particle counter during the first cryogenic operation of CSR in 2015.

  8. Single particle tracking-based reaction progress kinetic analysis reveals a series of molecular mechanisms of cetuximab-induced EGFR processes in a single living cell.

    Science.gov (United States)

    Kim, Do-Hyeon; Kim, Dong-Kyun; Zhou, Kai; Park, Soyeon; Kwon, Yonghoon; Jeong, Min Gyu; Lee, Nam Ki; Ryu, Sung Ho

    2017-07-01

    Cellular processes occur through the orchestration of multi-step molecular reactions. Reaction progress kinetic analysis (RPKA) can provide the mechanistic details to elucidate the multi-step molecular reactions. However, current tools have limited ability to simultaneously monitor dynamic variations in multiple complex states at the single molecule level to apply RPKA in living cells. In this research, a single particle tracking-based reaction progress kinetic analysis (sptRPKA) was developed to simultaneously determine the kinetics of multiple states of protein complexes in the membrane of a single living cell. The subpopulation ratios of different states were quantitatively (and statistically) reliably extracted from the diffusion coefficient distribution rapidly acquired by single particle tracking at constant and high density over a long period of time using super-resolution microscopy. Using sptRPKA, a series of molecular mechanisms of epidermal growth factor receptor (EGFR) cellular processing induced by cetuximab were investigated. By comprehensively measuring the rate constants and cooperativity of the molecular reactions involving four EGFR complex states, a previously unknown intermediate state was identified that represents the rate limiting step responsible for the selectivity of cetuximab-induced EGFR endocytosis to cancer cells.

  9. Single-particle potential of the Λ hyperon in nuclear matter with chiral effective field theory NLO interactions including effects of Y N N three-baryon interactions

    Science.gov (United States)

    Kohno, M.

    2018-03-01

    Adopting hyperon-nucleon and hyperon-nucleon-nucleon interactions parametrized in chiral effective field theory, single-particle potentials of the Λ and Σ hyperons are evaluated in symmetric nuclear matter and in pure neutron matter within the framework of lowest-order Bruckner theory. The chiral NLO interaction bears strong Λ N -Σ N coupling. Although the Λ potential is repulsive if the coupling is switched off, the Λ N -Σ N correlation brings about the attraction consistent with empirical data. The Σ potential is repulsive, which is also consistent with empirical information. The interesting result is that the Λ potential becomes shallower beyond normal density. This provides the possibility of solving the hyperon puzzle without introducing ad hoc assumptions. The effects of the Λ N N -Λ N N and Λ N N -Σ N N three-baryon forces are considered. These three-baryon forces are first reduced to normal-ordered effective two-baryon interactions in nuclear matter and then incorporated in the G -matrix equation. The repulsion from the Λ N N -Λ N N interaction is of the order of 5 MeV at normal density and becomes larger with increasing density. The effects of the Λ N N -Σ N N coupling compensate the repulsion at normal density. The net effect of the three-baryon interactions on the Λ single-particle potential is repulsive at higher densities.

  10. Study of single particle properties of neutron-rich Na isotopes on the "shore of the island of inversion" by means of neutron-transfer reactions

    CERN Multimedia

    Reiter, P; Blazhev, A A; Riisager, K; Bastin, B; Tengborn, E A; Kruecken, R; Voulot, D; Jeppesen, H B; Hadinia, B; Gernhaeuser, R A; Fynbo, H O U; Georgiev, G P; Habs, D; Fraile prieto, L M; Chapman, R; Nilsson, T; Diriken, J V J; Jenkins, D G; Kroell, T; Leske, J; Huyse, M L; Patronis, N

    We aim at the investigation of single particle properties of neutron-rich Na isotopes around the "shore of the island of inversion". As first experiment of this programme, we propose to study excited states in the isotope $^{29}$Na by a one-neutron transfer reaction with a $^{28}$Na beam at 3 MeV/u obtained from REX-ISOLDE impinging on a CD$_{2}$-target. The $\\gamma$-rays will be detected by the MINIBALL array and the particles by the T-REX array of segmented Si detectors. The main physics aims are to extract from the relative spectroscopic factors information on the configurations contributing to the wave functions of the populated states and, secondly, to identify and characterize negative parity states whose excitation energies reflect directly the N= 28 gap in this region. The results will be compared to recent shell model calculations involving new residual interactions. This will shed new light on the evolution of single particle structure and help to understand the underlying physics relevant for the f...

  11. In-situ determination of metallic variation and multi-association in single particles by combining synchrotron microprobe, sequential chemical extraction and multivariate statistical analysis.

    Science.gov (United States)

    Zhu, Yu-Min; Zhang, Hua; Fan, Shi-Suo; Wang, Si-Jia; Xia, Yi; Shao, Li-Ming; He, Pin-Jing

    2014-07-15

    Due to the heterogeneity of metal distribution, it is challenging to identify the speciation, source and fate of metals in solid samples at micro scales. To overcome these challenges single particles of air pollution control residues were detected in situ by synchrotron microprobe after each step of chemical extraction and analyzed by multivariate statistical analysis. Results showed that Pb, Cu and Zn co-existed as acid soluble fractions during chemical extraction, regardless of their individual distribution as chlorides or oxides in the raw particles. Besides the forms of Fe2O3, MnO2 and FeCr2O4, Fe, Mn, Cr and Ni were closely associated with each other, mainly as reducible fractions. In addition, the two groups of metals had interrelations with the Si-containing insoluble matrix. The binding could not be directly detected by micro-X-ray diffraction (μ-XRD) and XRD, suggesting their partial existence as amorphous forms or in the solid solution. The combined method on single particles can effectively determine metallic multi-associations and various extraction behaviors that could not be identified by XRD, μ-XRD or X-ray absorption spectroscopy. The results are useful for further source identification and migration tracing of heavy metals. Copyright © 2014 Elsevier B.V. All rights reserved.

  12. Multi-Mode Vibration Suppression in MIMO Systems by Extending the Zero Placement Input Shaping Technique: Applications to a 3-DOF Piezoelectric Tube Actuator

    Directory of Open Access Journals (Sweden)

    Yasser Al Hamidi

    2016-04-01

    Full Text Available Piezoelectric tube actuators are extensively used in scanning probe microscopes to provide dynamic scanning motions in open-loop operations. Furthermore, they are employed as micropositioners due to their high bandwidth, high resolution and ease of excitation. However, these piezoelectric micropositioners exhibit badly damped vibrations that occur when the input excites the dynamic response, which tends to degrade positioning accuracy and performance. This paper deals with vibrations’ feedforward control of a multi-degrees of freedom (DOF piezoelectric micropositioner in order to damp the vibrations in the direct axes and to reduce the cross-couplings. The novelty in this paper relative to the existing vibrations feedforward controls is the simplicity in design approach, the minimal number of shaper impulses for each input required to damp all modes of vibration at each output, and the account for the strong cross-couplings which only occur in multi-DOF cases. A generalization to a multiple degrees of freedom actuator is first proposed. Then simulation runs on a 3-DOF piezoelectric tube micropositioner have been effectuated to demonstrate the efficiency of the proposed method. Finally, experimental tests were carried out to validate and to confirm the predicted simulation.

  13. Coupling of collective and single-particle degrees of freedom in atomic nuclei (commentary to thesis qualifying for assistant-professorship)

    International Nuclear Information System (INIS)

    Chlebowska, D.

    1992-11-01

    The analysis of the spectroscopic properties of the spherical and transitional nuclei is performed from the point of view of the relation between the single-particle and collective degrees of freedom on the ground of the core-particle coupling model with the total angular momentum conserved and without any unphysical parameters (such as the attenuation parameter). A new definition of the rotational alignment is given. The staggering effect is interpreted as a manifestation of the vibrational structure. The rotational dependence of the energy gap parameter is shown to have an influence on the energy spectra of the transitional nuclei. The nuclei with A 130 are shown to have a tendency to be rather rigid. The vibrational and rotational structures, and the magnitude of the rotation-particle coupling in the considered nuclei are determined. 18 figs., 9 tabs., 66 refs. (author)

  14. Octupole deformation in neutron-rich actinides and superheavy nuclei and the role of nodal structure of single-particle wavefunctions in extremely deformed structures of light nuclei

    Science.gov (United States)

    Afanasjev, A. V.; Abusara, H.; Agbemava, S. E.

    2018-03-01

    Octupole deformed shapes in neutron-rich actinides and superheavy nuclei as well as extremely deformed shapes of the N∼ Z light nuclei have been investigated within the framework of covariant density functional theory. We confirmed the presence of new region of octupole deformation in neutron-rich actinides with the center around Z∼ 96,N∼ 196 but our calculations do not predict octupole deformation in the ground states of superheavy Z≥slant 108 nuclei. As exemplified by the study of 36Ar, the nodal structure of the wavefunction of occupied single-particle orbitals in extremely deformed structures allows to understand the formation of the α-clusters in very light nuclei, the suppression of the α-clusterization with the increase of mass number, the formation of ellipsoidal mean-field type structures and nuclear molecules.

  15. Band-head spectra of low-energy single-particle excitations in some well-deformed, odd-mass heavy nuclei within a microscopic approach

    Energy Technology Data Exchange (ETDEWEB)

    Koh, Meng-Hock [Universiti Teknologi Malaysia, Skudai, Johor (Malaysia); Univ. Bordeaux, CENBG, UMR5797, Gradignan (France); CNRS, IN2P3, CENBG, UMR5797, Gradignan (France); Duc, Dao Duy [Ton Duc Thang University, Division of Nuclear Physics, Ho Chi Minh City (Viet Nam); Ton Duc Thang University, Faculty of Applied Sciences, Ho Chi Minh City (Viet Nam); Nhan Hao, T.V. [Duy Tan University, Center of Research and Development, Danang (Viet Nam); Hue University, Center for Theoretical and Computational Physics, College of Education, Hue City (Viet Nam); Long, Ha Thuy [Hanoi University of Sciences, Vietnam National University, Hanoi (Viet Nam); Quentin, P. [Universiti Teknologi Malaysia, Skudai, Johor (Malaysia); Univ. Bordeaux, CENBG, UMR5797, Gradignan (France); CNRS, IN2P3, CENBG, UMR5797, Gradignan (France); Ton Duc Thang University, Division of Nuclear Physics, Ho Chi Minh City (Viet Nam); Bonneau, L. [Univ. Bordeaux, CENBG, UMR5797, Gradignan (France); CNRS, IN2P3, CENBG, UMR5797, Gradignan (France)

    2016-01-15

    In four well-deformed heavy odd nuclei, the energies of low-lying rotational band heads have been determined microscopically within a self-consistent Hartree-Fock-plus-BCS approach with blocking. A Skyrme nucleon-nucleon effective interaction has been used together with a seniority force to describe pairing correlations. Only such states which are phenomenologically deemed to be related to single-particle excitations have been considered. The polarization effects, including those associated with the genuine time-reversal symmetry breaking have been fully taken into account within our model assumptions. The calculated spectra are in reasonably good qualitative agreement with available data for the considered odd-neutron nuclei. This is not so much the case for the odd-proton nuclei. A potential explanation for such a difference in behavior is proposed. (orig.)

  16. Single-particle measurements of bouncing particles and in situ collection efficiency from an airborne aerosol mass spectrometer (AMS) with light-scattering detection

    Science.gov (United States)

    Liao, Jin; Brock, Charles A.; Murphy, Daniel M.; Sueper, Donna T.; Welti, André; Middlebrook, Ann M.

    2017-10-01

    A light-scattering module was coupled to an airborne, compact time-of-flight aerosol mass spectrometer (LS-AMS) to investigate collection efficiency (CE) while obtaining nonrefractory aerosol chemical composition measurements during the Southeast Nexus (SENEX) campaign. In this instrument, particles scatter light from an internal laser beam and trigger saving individual particle mass spectra. Nearly all of the single-particle data with mass spectra that were triggered by scattered light signals were from particles larger than ˜ 280 nm in vacuum aerodynamic diameter. Over 33 000 particles are characterized as either prompt (27 %), delayed (15 %), or null (58 %), according to the time and intensity of their total mass spectral signals. The particle mass from single-particle spectra is proportional to that derived from the light-scattering diameter (dva-LS) but not to that from the particle time-of-flight (PToF) diameter (dva-MS) from the time of the maximum mass spectral signal. The total mass spectral signal from delayed particles was about 80 % of that from prompt ones for the same dva-LS. Both field and laboratory data indicate that the relative intensities of various ions in the prompt spectra show more fragmentation compared to the delayed spectra. The particles with a delayed mass spectral signal likely bounced off the vaporizer and vaporized later on another surface within the confines of the ionization source. Because delayed particles are detected by the mass spectrometer later than expected from their dva-LS size, they can affect the interpretation of particle size (PToF) mass distributions, especially at larger sizes. The CE, measured by the average number or mass fractions of particles optically detected that had measurable mass spectra, varied significantly (0.2-0.9) in different air masses. The measured CE agreed well with a previous parameterization when CE > 0.5 for acidic particles but was sometimes lower than the minimum parameterized CE of 0.5.

  17. Single particle ICP-MS as a tool for determining the stability of silver nanoparticles in aquatic matrixes under various environmental conditions, including treatment by ozonation.

    Science.gov (United States)

    Telgmann, Lena; Nguyen, Michael Thanh Khoa; Shen, Li; Yargeau, Viviane; Hintelmann, Holger; Metcalfe, Chris D

    2016-07-01

    Silver nanoparticles (AgNPs) are used in a large number of consumer products due to their antimicrobial and antifungal properties, and these materials may be discharged into municipal wastewater. Wastewater treatment, including advanced oxidation processes (AOPs), may modify the forms of silver in wastewater before they are discharged into surface waters. In addition, little is known about the changes in AgNPs that occur in natural waters under different environmental conditions. In this project, we utilized single particle ICP-MS (spICP-MS) and dynamic light scattering (DLS) analytical techniques to evaluate changes in the number and size of AgNPs in laboratory experiments with milliQ water under different environmental conditions, as well as during ozonation. Changes in the number and size of AgNPs determined by spICP-MS were evidence of altered stability of the nanoparticles. Increased rates of dissolution occurred under extremes of pH. Lower temperature decreased the rate of dissolution of AgNP relative to the dissolution in treatments at room temperature. The addition of chloride resulted in the loss of AgNPs from suspension due to agglomeration and precipitation. Ozonation led to a rapid decline in the number and size of AgNPs, as indicated by both spICP-MS and DLS analysis. An increase in the concentration of dissolved silver in the ozone treatments was evidence that changes in particle size were a result of oxidative dissolution of AgNPs to silver ion. Graphical abstract Single particle ICP-MS is used to evaluate dissolution of silver nanoparticles under different environmental conditions, including water treatment by ozonation.

  18. Online differentiation of mineral phase in aerosol particles by ion formation mechanism using a LAAP-TOF single-particle mass spectrometer

    Directory of Open Access Journals (Sweden)

    N. A. Marsden

    2018-01-01

    Full Text Available Mineralogy of silicate mineral dust has a strong influence on climate and ecosystems due to variation in physiochemical properties that result from differences in composition and crystal structure (mineral phase. Traditional offline methods of analysing mineral phase are labour intensive and the temporal resolution of the data is much longer than many atmospheric processes. Single-particle mass spectrometry (SPMS is an established technique for the online size-resolved measurement of particle composition by laser desorption ionisation (LDI followed by time-of-flight mass spectrometry (TOF-MS. Although non-quantitative, the technique is able to identify the presence of silicate minerals in airborne dust particles from markers of alkali metals and silicate molecular ions in the mass spectra. However, the differentiation of mineral phase in silicate particles by traditional mass spectral peak area measurements is not possible. This is because instrument function and matrix effects in the ionisation process result in variations in instrument response that are greater than the differences in composition between common mineral phases.In this study, we introduce a novel technique that enables the differentiation of mineral phase in silicate mineral particles by ion formation mechanism measured from subtle changes in ion arrival times at the TOF-MS detector. Using a combination of peak area and peak centroid measurements, we show that the arrangement of the interstitial alkali metals in the crystal structure, an important property in silicate mineralogy, influences the ion arrival times of elemental and molecular ion species in the negative ion mass spectra. A classification scheme is presented that allowed for the differentiation of illite–smectite, kaolinite and feldspar minerals on a single-particle basis. Online analysis of mineral dust aerosol generated from clay mineral standards produced mineral fractions that are in agreement with bulk

  19. Online differentiation of mineral phase in aerosol particles by ion formation mechanism using a LAAP-TOF single-particle mass spectrometer

    Science.gov (United States)

    Marsden, Nicholas A.; Flynn, Michael J.; Allan, James D.; Coe, Hugh

    2018-01-01

    Mineralogy of silicate mineral dust has a strong influence on climate and ecosystems due to variation in physiochemical properties that result from differences in composition and crystal structure (mineral phase). Traditional offline methods of analysing mineral phase are labour intensive and the temporal resolution of the data is much longer than many atmospheric processes. Single-particle mass spectrometry (SPMS) is an established technique for the online size-resolved measurement of particle composition by laser desorption ionisation (LDI) followed by time-of-flight mass spectrometry (TOF-MS). Although non-quantitative, the technique is able to identify the presence of silicate minerals in airborne dust particles from markers of alkali metals and silicate molecular ions in the mass spectra. However, the differentiation of mineral phase in silicate particles by traditional mass spectral peak area measurements is not possible. This is because instrument function and matrix effects in the ionisation process result in variations in instrument response that are greater than the differences in composition between common mineral phases.In this study, we introduce a novel technique that enables the differentiation of mineral phase in silicate mineral particles by ion formation mechanism measured from subtle changes in ion arrival times at the TOF-MS detector. Using a combination of peak area and peak centroid measurements, we show that the arrangement of the interstitial alkali metals in the crystal structure, an important property in silicate mineralogy, influences the ion arrival times of elemental and molecular ion species in the negative ion mass spectra. A classification scheme is presented that allowed for the differentiation of illite-smectite, kaolinite and feldspar minerals on a single-particle basis. Online analysis of mineral dust aerosol generated from clay mineral standards produced mineral fractions that are in agreement with bulk measurements reported by

  20. Activation energy for mobility of dyes and proteins in polymer solutions: from diffusion of single particles to macroscale flow.

    Science.gov (United States)

    Sozański, Krzysztof; Wiśniewska, Agnieszka; Kalwarczyk, Tomasz; Hołyst, Robert

    2013-11-27

    We measure the activation energy Ea for the diffusion of molecular probes (dyes and proteins of radii from 0.52 to 6.9 nm) and for macroscopic flow in a model complex liquid-aqueous solutions of polyethylene glycol. We cover a broad range of polymer molecular weights, concentrations, and temperatures. Fluorescence correlation spectroscopy and rheometry experiments reveal a relationship between the excess of the activation energy in polymer solutions over the one in pure solvent ΔEa and simple parameters describing the structure of the system: probe radius, polymer hydrodynamic radius, and correlation length. ΔEa varies by more than an order of magnitude in the investigated systems (in the range of ca. 1-15 kJ/mol) and for probes significantly larger than the polymer hydrodynamic radius approaches the value measured for macroscopic flow. We develop an explicit formula describing the smooth transition of ΔEa from the diffusion of molecular probes to macroscopic flow. This formula is a reference for the quantitative analysis of specific interactions of moving nano-objects with their environment as well as active transport. For instance, the power developed by a molecular motor moving at constant velocity u is proportional to u2exp(Ea/RT).

  1. Evaluation of Different Single-Walled Carbon Nanotube Surface Coatings for Single-Particle Tracking Applications in Biological Environments.

    Science.gov (United States)

    Gao, Zhenghong; Danné, Noémie; Godin, Antoine Guillaume; Lounis, Brahim; Cognet, Laurent

    2017-11-16

    Fluorescence imaging of biological systems down to the single-molecule level has generated many advances in cellular biology. For applications within intact tissue, single-walled carbon nanotubes (SWCNTs) are emerging as distinctive single-molecule nanoprobes, due to their near-infrared photoluminescence properties. For this, SWCNT surfaces must be coated using adequate molecular moieties. Yet, the choice of the suspension agent is critical since it influences both the chemical and emission properties of the SWCNTs within their environment. Here, we compare the most commonly used surface coatings for encapsulating photoluminescent SWCNTs in the context of bio-imaging applications. To be applied as single-molecule nanoprobes, encapsulated nanotubes should display low cytotoxicity, and minimal unspecific interactions with cells while still being highly luminescent so as to be imaged and tracked down to the single nanotube level for long periods of time. We tested the cell proliferation and cellular viability of each surface coating and evaluated the impact of the biocompatible surface coatings on nanotube photoluminescence brightness. Our study establishes that phospholipid-polyethylene glycol-coated carbon nanotube is the best current choice for single nanotube tracking experiments in live biological samples.

  2. Resonant pickups for non-destructive single-particle detection in heavy-ion storage rings and first experimental results

    Energy Technology Data Exchange (ETDEWEB)

    Sanjari, Mohammad Shahab

    2013-04-26

    Nuclear astrophysics studies on highly charged radionuclides benefit from accelerator facilities with storage rings, where exotic nuclides produced with small yields can be efficiently investigated. Currently there are two accelerator facilities capable of storing highly charged heavy ions, GSI in Darmstadt and IMP in Lanzhou. Non-destructive detection methods are often used for in-flight measurements based on frequency analysis. The sensitivity of such detection systems are of primary importance specially when number of stored ions is small. Furthermore, since the exotic nuclides of interest are as a rule short-lived, the detectors must be fast. One common form of such detectors are parallel plate SCHOTTKY monitors, on which particles induce a mirror charge at each passage. This method has been successfully used at ESR experimental storage ring of GSI since 1991. In this work we describe a new resonant SCHOTTKY pickup operating as a high sensitive cavity current monitor which was mounted and commissioned in the ESR early 2010. It was successfully used in several storage ring experiments. A very similar pickup was mounted in CSRe at IMP Lanzhou in 2011. First in-ring tests have been performed and new experimental results are pending. The spectral analysis of acquired signals by the new detector has enabled a broad range of new physics experiments. The theory of operation and first experimental results and future perspectives are presented in this thesis.

  3. Probing the Evaporation Dynamics of Mixed SOA/Squalane Particles Using Size-Resolved Composition and Single-Particle Measurements.

    Science.gov (United States)

    Robinson, Ellis Shipley; Saleh, Rawad; Donahue, Neil M

    2015-08-18

    An analysis of the formation and evaporation of mixed-particles containing squalane (a surrogate for hydrophobic primary organic aerosol, POA) and secondary organic aerosol (SOA) is presented. In these experiments, one material (D62-squalane or SOA from α-pinene + O3) was prepared first to serve as surface area for condensation of the other, forming the mixed-particles. The mixed-particles were then subjected to a heating-ramp from 22 to 44 °C. We were able to determine that (1) almost all of the SOA mass is comprised of material less volatile than D62-squalane; (2) AMS collection efficiency in these mixed-particle systems can be parametrized as a function of the relative mass fraction of the components; and (3) the vast majority of D62-squalane is able to evaporate from the mixed particles, and does so on the same time scale regardless of the order of preparation. We also performed two-population mixing experiments to directly test whether D62-squalane and SOA from α-pinene + O3 form a single solution or two separate phases. We find that these two OA types are immiscible, which informs our inference of the morphology of the mixed-particles. If the morphology is core-shell and dictated by the order of preparation, these data indicate that squalane is able to diffuse relatively quickly through the SOA shell, implying that there are no major diffusion limitations.

  4. On the simultaneous deployment of two single-particle mass spectrometers at an urban background and a roadside site during SAPUSS

    Science.gov (United States)

    Dall'Osto, Manuel; Beddows, David C. S.; McGillicuddy, Eoin J.; Esser-Gietl, Johanna K.; Harrison, Roy M.; Wenger, John C.

    2016-08-01

    The aerosol time-of-flight mass spectrometer (ATOFMS) provides size-resolved information on the chemical composition of single particles with high time resolution. Within SAPUSS (Solving Aerosol Problems by Using Synergistic Strategies), continuous ATOFMS measurements of ambient particles were made simultaneously at two urban locations: urban background (UB) site and roadside (RS) site in the city of Barcelona (Spain) from 17 September to 18 October 2010. Two different instrumental configurations were used: ATOFMS (TSI 3800) with a converging nozzle inlet (high efficiency at about 800-2000 nm) at the UB site and ATOFMS (TSI 3800-100) with an aerodynamic lens inlet (high efficiency at about 300-700 nm) at the RS site. This is the first time, to our knowledge, that two ATOFMS instruments have been deployed in the same field study. The different instrument configurations had an impact on the observed particle types at the two sites. Nevertheless, 10 particle types were detected at both locations, including local and regional elemental carbon (22.7-58.9 % of total particles), fresh and aged sea salt (1.0-14.6 %), local and regional nitrate-containing aerosols (3-11.6 %), local lead-containing metallic particles (0.1-0.2 %), and transported Fe-nitrate particles (0.8-2.5 %). The ATOFMS at the UB also characterized four particle types: calcium-containing dust (0.9 %), Saharan dust (1.3 %), vanadium-containing particles (0.9 %), and vegetative debris (1.7 %). By contrast, the high statistical counts of fine particles detected at the RS allowed identification of eight particle types. Four of these contained organic nitrogen of primary and secondary origin, which highlights the complex nature of the sources and processes that contribute to this aerosol chemical component. Aminium salts were found related to coarse sulfate-rich particle types, suggesting heterogeneous reaction mechanisms for their formation. The other four particle types mainly containing organic carbon were

  5. Single-particle characterization of municipal solid waste (MSW) ash particles using low- Z particle electron probe X-ray microanalysis

    Science.gov (United States)

    Hwang, HeeJin; Ro, Chul-Un

    Environmentally benign treatment of municipal solid waste (MSW) ashes has been a worldwide issue since more countries are implementing incineration to reduce waste volume. A single-particle analytical technique, named low- Z particle electron probe X-ray microanalysis (low- Z particle EPMA) was applied to characterize MSW fly- and bottom-ash particle samples collected from two municipal incinerators in Korea. According to their chemical composition, many distinctive particle types were identified. For fly ash sample collected in one incinerator (sample S1), where lime slurry injection is used for acid-gas treatment, CaCO 3-containing particles (28.4%) are the most abundantly encountered, followed by carbonaceous (23.6%), SiO 2-containing (13.8%), NaCl-containing (13.1%), and iron-containing (10.5%) particles. For fly ash sample collected at the other incinerator (sample S2), NaCl-containing particles (40.4%) are the most abundantly encountered, followed by iron-containing (29.1%), carbonaceous (11.8%), CaCO 3-containing (2.2%), and SiO 2-containing (7.0%) particles. For bottom ash sample collected at one incinerator (sample S3), iron-containing particles (46.6%) are the most abundantly encountered, followed by CaCO 3-containing (17.3%), carbonaceous (16.6%), and Si and/or Al oxide-containing (15.8%) particles. For bottom ash sample collected in the other incinerator (sample S4), iron-containing particles (63.4%) are also the most abundantly encountered, followed by carbonaceous (14.0%), CaCO 3-containing (10.0%), and Si and/or Al oxide-containing (6.1%) particles. Chemical compositions of the two bottom ash samples are not much different compared to those of the two fly ash samples. It was demonstrated that the single-particle characterization using this low- Z particle EPMA technique provided detailed information on various types of chemical species in the MSW ash samples. In addition, the technique has advantage over conventional analytical techniques in the

  6. Single-particle characterization of biomass burning organic aerosol (BBOA: evidence for non-uniform mixing of high molecular weight organics and potassium

    Directory of Open Access Journals (Sweden)

    A. K. Y. Lee

    2016-05-01

    Full Text Available Biomass burning organic aerosol (BBOA can be emitted from natural forest fires and human activities such as agricultural burning and domestic energy generation. BBOA is strongly associated with atmospheric brown carbon (BrC that absorbs near-ultraviolet and visible light, resulting in significant impacts on regional visibility degradation and radiative forcing. The mixing state of BBOA can play a critical role in the prediction of aerosol optical properties. In this work, single-particle measurements from a Soot-Particle Aerosol Mass Spectrometer coupled with a light scattering module (LS-SP-AMS were performed to examine the mixing state of BBOA, refractory black carbon (rBC, and potassium (K, a tracer for biomass burning aerosol in an air mass influenced by wildfire emissions transported from northern Québec to Toronto, representing aged biomass burning plumes. Cluster analysis of single-particle measurements identified five BBOA-related particle types. rBC accounted for 3–14 wt % of these particle types on average. Only one particle type exhibited a strong ion signal for K+, with mass spectra characterized by low molecular weight organic species. The remaining four particle types were classified based on the apparent molecular weight of the BBOA constituents. Two particle types were associated with low potassium content and significant amounts of high molecular weight (HMW organic compounds. Our observations indicate non-uniform mixing of particles within a biomass burning plume in terms of molecular weight and illustrate that HMW BBOA can be a key contributor to low-volatility BrC observed in BBOA particles. The average mass absorption efficiency of low-volatility BBOA is about 0.8–1.1 m2 g−1 based on a theoretical closure calculation. Our estimates indicate that low-volatility BBOA contributes ∼ 33–44 % of thermo-processed particle absorption at 405 nm; and almost all of the BBOA absorption was associated with low

  7. Gold nanoparticles with tipped surface structures as substrates for single-particle surface-enhanced Raman spectroscopy: concave nanocubes, nanotrisoctahedra, and nanostars.

    Science.gov (United States)

    Zhang, Qingfeng; Large, Nicolas; Wang, Hui

    2014-10-08

    We demonstrate that Au nanoparticles with tipped surface structures, such as concave nanocubes, nanotrisoctahedra, and nanostars, possess size-dependent tunable plasmon resonances and intense near-field enhancements exploitable for single-particle surface-enhanced Raman spectroscopy (spSERS) under near-infrared excitation. We report a robust seed-mediated growth method for the selective fabrication of Au concave nanocubes, nanotrisoctahedra, and nanostars with fine-controlled particle sizes and narrow size distributions. Through tight control over particle sizes, the plasmon resonances of the nanoparticles can be fine-tuned over a broad spectral range with respect to the excitation laser, allowing us to systematically quantify the SERS enhancements on individual nanoparticles as a function of particle size for each particle geometry. Understanding of the geometry-dependent plasmonic characteristics and SERS activities of the nanoparticles is further enhanced by finite-difference time-domain (FDTD) calculations. Our results clearly show that strong SERS enhancements can be obtained and further optimized on individual Au nanoparticles with nanoengineered "hot spots" on their tipped surfaces when the plasmon resonances of the nanoparticles are tuned to the optimal spectral regions with respect to the excitation laser wavelength. Using tunable plasmonic nanoparticles with tipped surface structures as substrates for spSERS represents a highly promising and feasible approach to the optimization of SERS-based sensing and imaging applications.

  8. Characterization of a detector chain using a FPGA-based time-to-digital converter to reconstruct the three-dimensional coordinates of single particles at high flux

    Energy Technology Data Exchange (ETDEWEB)

    Nogrette, F.; Chang, R.; Bouton, Q.; Westbrook, C. I.; Clément, D. [Laboratoire Charles Fabry, Institut d’Optique Graduate School, CNRS, Univ. Paris-Saclay, 91127 Palaiseau cedex (France); Heurteau, D.; Sellem, R. [Fédération de Recherche LUMAT (DTPI), CNRS, Univ. Paris-Sud, Institut d’Optique Graduate School, Univ. Paris-Saclay, F-91405 Orsay (France)

    2015-11-15

    We report on the development of a novel FPGA-based time-to-digital converter and its implementation in a detection chain that records the coordinates of single particles along three dimensions. The detector is composed of micro-channel plates mounted on top of a cross delay line and connected to fast electronics. We demonstrate continuous recording of the timing signals from the cross delay line at rates up to 4.1 × 10{sup 6} s{sup −1} and three-dimensional reconstruction of the coordinates up to 3.2 × 10{sup 6} particles per second. From the imaging of a calibrated structure we measure the in-plane resolution of the detector to be 140(20) μm at a flux of 3 × 10{sup 5} particles per second. In addition, we analyze a method to estimate the resolution without placing any structure under vacuum, a significant practical improvement. While we use UV photons here, the results of this work apply to the detection of other kinds of particles.

  9. Signatures for shape-phase transitions in the rare-earth nuclei, in the evolution of single-particle spectra and two-particle transfer-intensities

    Energy Technology Data Exchange (ETDEWEB)

    Fossion, R. [Instituto de Ciencias Nucleares, UNAM, Apartado Postal 70-543, 04510 Mexico D. F. (Mexico)

    2008-12-15

    The rare-earth Nd, Sm, Gd and Dy nuclei are well known to undergo a shape-phase transition around N {approx} 90 from vibrational to rotational behaviour - or, correspondingly - from a spherical nuclear shape to an axial-symmetric deformed shape. This can experimentally be verified by, for example, the evolution of the R{sub 4/2} = E(4{sup +}{sub 1})/E(2{sup +}{sub 1}) ratio, or the evolution of the quadrupole moment, Q{sub 2}. Recently, the study o nuclear phase-shape transitions has gained considerable interest, since the introduction of exact algebraic solutions for the critical points many of the different phase-shape transitions that are possible in the atomic nucleus. In this contribution, in the first part, we investigate the microscopic underlying mechanism that drives the rare-earth isotopes towards deformation, studying the evolution of their proton an neutron single-particle spectra within the Relativistic Hartree-Bogoliubov model. In the second part, within the Interacting Boson Model and the framework with boson coherent states, and in the light of renewed interest in experiments on two-particle transfer-reactions, we study the evolution of the transfer spectroscopic intensities as a possible signature of shape-phase transitions. (Author)

  10. Charge trapping and storage by composite P3HT/PC60BM nanoparticles investigated by fluorescence-voltage/single particle spectroscopy.

    Science.gov (United States)

    Hu, Zhongjian; Gesquiere, Andre J

    2011-12-28

    Fluorescence-voltage/single particle spectroscopy (F-V/SPS) was employed to study exciton-hole polaron interactions and interfacial charge transfer processes for pure poly(3-hexylthiophene) (P3HT) nanoparticles (NPs) and composite P3HT/PC(60)BM NPs in functioning hole-injection devices. F-V/SPS data collected on a particle-by-particle basis reveal an apparent bistability in the fluorescence-voltage modulation curves for composite NPs of P3HT and [6,6]-phenyl-C(61)-butyric acid methyl ester (PC(60)BM) that is absent for pure P3HT NPs. A pronounced deep trapping of free electrons photogenerated from the composite P3HT/PC(60)BM NPs at the NP/dielectric interface and hole trapping by fullerene anions in composite P3HT/PC(60)BM NPs under photoexcitation lies at the basis of this finding. The deep electron trapping effect reported here for composite conjugated polymer/fullerene NPs presents an opportunity for future application of these NPs in nanoscale memory and imaging devices. © 2011 American Chemical Society

  11. Simultaneous characterisation of silver nanoparticles and determination of dissolved silver in chicken meat subjected to in vitro human gastrointestinal digestion using single particle inductively coupled plasma mass spectrometry.

    Science.gov (United States)

    Ramos, K; Ramos, L; Gómez-Gómez, M M

    2017-04-15

    In this study, a chicken meat containing AgNPs (candidate reference material Nanolyse 14) has been used as a model matrix to study the fate and behaviour of AgNPs upon oral ingestion following an in vitro model that included saliva, gastric and intestinal digestions. The behaviour of a 40nm AgNPs standard solution during the three digestion steps was also evaluated. Sample preparation conditions were optimised to prevent AgNPs oxidation and/or aggregation and to ensure the representativeness of the reported results. Total silver released from the test sample and the evaluated AgNP standard was determined by inductively coupled plasma mass spectrometry (ICPMS). The presence of both AgNPs and dissolved silver in the extracts was confirmed by single particle (SP)-ICPMS analysis. AgNPs were sized and the particle number concentration determined in the three digestion juices. Experimental results demonstrated differentiated behaviours for AgNP from the standard solution and the meat sample highlighting the relevance of using physiological conditions for accurate risk assessment. In the most realistic scenario assayed (i.e., spiked chicken meat analysis), only 13% of the AgNPs present in the reference material would reach the intestine wall. Meanwhile, other bioaccessible dissolved forms of silver would account for as much as 44% of the silver initially spiked to the meat paste. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Fractionation and Characterization of High Aspect Ratio Gold Nanorods Using Asymmetric-Flow Field Flow Fractionation and Single Particle Inductively Coupled Plasma Mass Spectrometry

    Directory of Open Access Journals (Sweden)

    Thao M. Nguyen

    2015-07-01

    Full Text Available Gold nanorods (GNRs are of particular interest for biomedical applications due to their unique size-dependent longitudinal surface plasmon resonance band in the visible to near-infrared. Purified GNRs are essential for the advancement of technologies based on these materials. Used in concert, asymmetric-flow field flow fractionation (A4F and single particle inductively coupled mass spectrometry (spICP-MS provide unique advantages for fractionating and analyzing the typically complex mixtures produced by common synthetic procedures. A4F fractions collected at specific elution times were analyzed off-line by spICP-MS. The individual particle masses were obtained by conversion of the ICP-MS pulse intensity for each detected particle event, using a defined calibration procedure. Size distributions were then derived by transforming particle mass to length assuming a fixed diameter. The resulting particle lengths correlated closely with ex situ transmission electron microscopy. In contrast to our previously reported observations on the fractionation of low-aspect ratio (AR GNRs (AR < 4, under optimal A4F separation conditions the results for high-AR GNRs of fixed diameter (≈20 nm suggest normal, rather than steric, mode elution (i.e., shorter rods with lower AR generally elute first. The relatively narrow populations in late eluting fractions suggest the method can be used to collect and analyze specific length fractions; it is feasible that A4F could be appropriately modified for industrial scale purification of GNRs.

  13. "Depth-profiling" and quantitative characterization of the size, composition, shape, density, and morphology of fine particles with SPLAT, a single-particle mass spectrometer.

    Science.gov (United States)

    Zelenyuk, Alla; Yang, Juan; Song, Chen; Zaveri, Rahul A; Imre, Dan

    2008-01-31

    A significant fraction of atmospheric particles are composed of inorganic substances that are mixed or coated with organic compounds. The properties and behavior of these particles depend on the internal composition and arrangement of the specific constituents in each particle. It is important to know which constituent is on the surface and whether it covers the particle surface partially or entirely. We demonstrate here an instrument consisting of an ultrasensitive single-particle mass spectrometer coupled with a differential mobility analyzer to quantitatively measure in real time individual particle composition, size, density, and shape and to determine which substance is on the surface and whether it entirely covers the particle. For this study, we use NaCl particles completely coated with liquid dioctyl phthalate to generate spherical particles, and NaCl particles partially coated with pyrene, a solid poly aromatic hydrocarbon, to produce aspherical particles with pyrene nodules and an exposed NaCl core. We show that the behavior of the mass spectral intensities as a function of laser fluence yields information that can be used to determine the morphological distribution of individual particle constituents.

  14. Characterization of gold nanoparticle uptake by tomato plants using enzymatic extraction followed by single-particle inductively coupled plasma-mass spectrometry analysis.

    Science.gov (United States)

    Dan, Yongbo; Zhang, Weilan; Xue, Runmiao; Ma, Xingmao; Stephan, Chady; Shi, Honglan

    2015-03-03

    Plant uptake and accumulation of nanoparticles (NPs) represent an important pathway for potential human expose to NPs. Consequently, it is imperative to understand the uptake of accumulation of NPs in plant tissues and their unique physical and chemical properties within plant tissues. Current technologies are limited in revealing the unique characteristics of NPs after they enter plant tissues. An enzymatic digestion method, followed by single-particle inductively coupled plasma-mass spectrometry (SP-ICP-MS) analysis, was developed for simultaneous determination of gold NP (AuNP) size, size distribution, particle concentration, and dissolved Au concentration in tomato plant tissues. The experimental results showed that Macerozyme R-10 enzyme was capable of extracting AuNPs from tomato plants without causing dissolution or aggregation of AuNPs. The detection limit for quantification of AuNP size was 20 nm, and the AuNP particle concentration detection limit was 1000 NPs/mL. The particle concentration recoveries of spiked AuNPs were high (79-96%) in quality control samples. The developed SP-ICP-MS method was able to accurately measure AuNP size, size distribution, and particle concentration in the plant matrix. The dosing study indicated that tomato can uptake AuNPs as intact particles without alternating the AuNP properties.

  15. Direct quantitative screening of influenza A virus without DNA amplification by single-particle dual-mode total internal reflection scattering.

    Science.gov (United States)

    Lee, Seungah; Chakkarapani, Suresh Kumar; Yeung, Edward S; Kang, Seong Ho

    2017-01-15

    Quantitative screening of influenza A (H7N9) virus without DNA amplification was performed based on single-particle dual-mode total internal reflection scattering (SD-TIRS) with a transmission grating (TG). A gold nanopad was utilized as a substrate for the hybridization of probe DNA molecules with the TIRS nanotag (silver-nanoparticle). The TG effectively isolated the scattering signals in first-order spectral images (n=+1) of the nanotag from that of the substrate, providing excellent enhancement of signal-to-noise and selectivity. By using single-DNA molecule/TIRS nanotag hybridization, target DNA molecules of H7N9 were detected down to 74 zM, which is at least 100,000 times lower than the current detection limit of 9.4fM. By simply modifying the design of the probe DNA molecules, this technique can be used to directly screen other viral DNAs in various human biological samples at the single-molecule level without target amplification. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. Assessing Single Particle Soot Photometer and Integrating Sphere/Integrating Sandwich Spectrophotometer measurement techniques for quantifying black carbon concentration in snow

    Directory of Open Access Journals (Sweden)

    J. P. Schwarz

    2012-11-01

    Full Text Available We evaluate the performance of the Single Particle Soot Photometer (SP2 and the Integrating Sphere/Integrating Sandwich Spectrophotometer (ISSW in quantifying the concentration of refractory black carbon (BC in snow samples. We find that the SP2 can be used to measure BC mass concentration in snow with substantially larger uncertainty (60% than for atmospheric sampling (<30%. Achieving this level of accuracy requires careful assessment of nebulizer performance and SP2 calibration with consideration of the fact that BC in snow can exist in larger sizes than typically observed in the atmosphere. Once these issues are addressed, the SP2 is able to measure the size distribution and mass concentration of BC in the snow. Laboratory comparison of the SP2 and the ISSW revealed significant biases in the estimate of BC concentration from the ISSW when test samples contained dust or non-absorbing particulates. These results suggest that current estimates of BC mass concentration in snow or ice formed from fallen snow using either the SP2 or the ISSW may be associated with significant underestimates of uncertainty.

  17. Chemical Synthesis of K34-Ubiquitylated H2B for Nucleosome Reconstitution and Single-Particle Cryo-Electron Microscopy Structural Analysis.

    Science.gov (United States)

    Li, Jiabin; He, Qiaoqiao; Liu, Yuntao; Liu, Sanling; Tang, Shan; Li, Chengmin; Sun, Demeng; Li, Xiaorun; Zhou, Min; Zhu, Ping; Bi, Guoqiang; Zhou, Zhenghong; Zheng, Ji-Shen; Tian, Changlin

    2017-01-17

    Post-translational modifications (e.g., ubiquitylation) of histones play important roles in dynamic regulation of chromatin. Histone ubiquitylation has been speculated to directly influence the structure and dynamics of nucleosomes. However, structural information for ubiquitylated nucleosomes is still lacking. Here we report an alternative strategy for total chemical synthesis of homogenous histone H2B-K34-ubiquitylation (H2B-K34Ub) by using acid-cleavable auxiliary-mediated ligation of peptide hydrazides for site-specific ubiquitylation. Synthetic H2B-K34Ub was efficiently incorporated into nucleosomes and further used for single-particle cryo-electron microscopy (cryo-EM) imaging. The cryo-EM structure of the nucleosome containing H2B-K34Ub suggests that two flexible ubiquitin domains protrude between the DNA chains of the nucleosomes. The DNA chains around the H2B-K34 sites shift and provide more space for ubiquitin to protrude. These analyses indicated local and slight structural influences on the nucleosome with ubiquitylation at the H2B-K34 site. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. [Seasonal Variation Characteristics and Potential Source Contribution of Sulfate, Nitrate and Ammonium in Beijing by Using Single Particle Aerosol Mass Spectrometry].

    Science.gov (United States)

    Liu, Lang; Zhang, Wen-jie; Du, Shi-yong; Hou, Lu-jian; Han, Bin; Yang, Wen; Chen, Min-dong; Bai, Zhi-peng

    2016-05-15

    Single particle aerosol mass spectrometry (SPAMS) was deployed to continuously observe the aerosol particles of Beijing urban area from 2013-12 to 2014-11, and the hourly average data of sulfate, nitrate and ammonium (SNA) were obtained using the characteristic ion tracer method. The mixing state and size distribution of SNA were analyzed. In addition, based on Hysplit 48 h back air mass trajectory results in combination with Concentration Weighted Trajectory method (CWT), we obtained the seasonal potential source contribution area of SNA. The results showed that the mixture of sulfate, nitrate and ammonium in spring and summer was more stable than that in autumn and winter. The size distribution of sulfate and nitrate was very similar. The size distribution characteristics of SNA followed the order of autumn > summer > spring > winter. The potential source region of SNA had similar spatial distribution characteristics, and the potential source region of SNA was mainly located in Beijing and south areas, especially at Tianjin, Langfang, Hengshui, Baoding and Shijiazhuang.

  19. Single particle electron microscopy analysis of the bovine anion exchanger 1 reveals a flexible linker connecting the cytoplasmic and membrane domains.

    Directory of Open Access Journals (Sweden)

    Jiansen Jiang

    Full Text Available Anion exchanger 1 (AE1 is the major erythrocyte membrane protein that mediates chloride/bicarbonate exchange across the erythrocyte membrane facilitating CO₂ transport by the blood, and anchors the plasma membrane to the spectrin-based cytoskeleton. This multi-protein cytoskeletal complex plays an important role in erythrocyte elasticity and membrane stability. An in-frame AE1 deletion of nine amino acids in the cytoplasmic domain in a proximity to the membrane domain results in a marked increase in membrane rigidity and ovalocytic red cells in the disease Southeast Asian Ovalocytosis (SAO. We hypothesized that AE1 has a flexible region connecting the cytoplasmic and membrane domains, which is partially deleted in SAO, thus causing the loss of erythrocyte elasticity. To explore this hypothesis, we developed a new non-denaturing method of AE1 purification from bovine erythrocyte membranes. A three-dimensional (3D structure of bovine AE1 at 2.4 nm resolution was obtained by negative staining electron microscopy, orthogonal tilt reconstruction and single particle analysis. The cytoplasmic and membrane domains are connected by two parallel linkers. Image classification demonstrated substantial flexibility in the linker region. We propose a mechanism whereby flexibility of the linker region plays a critical role in regulating red cell elasticity.

  20. Tensor force effect on the evolution of single-particle energies in some isotopic chains in the relativistic Hartree-Fock approximation

    Science.gov (United States)

    López-Quelle, M.; Marcos, S.; Niembro, R.; Savushkin, L. N.

    2018-03-01

    Within a nonlinear relativistic Hartree-Fock approximation combined with the BCS method, we study the effect of the nucleon-nucleon tensor force of the π-exchange potential on the spin- and pseudospin-orbit doublets along the Ca and Sn isotopic chains. We show how the self-consistent tensor force effect modifies the splitting of both kinds of doublets in an interdependent form, leading, quite generally, to opposite effects in the accomplishment of the spin and pseudospin symmetries (the one is restored, the other one deteriorates and vice versa). The ordering of the single-particle energy levels is crucial to this respect. Also, we observe a mutual dependence on the evolution of the shell closure gap Z = 50 and the energy band outside the core, along the Sn chain, as due to the tensor force. In fact, when the shell gap is quenched the outside energy band is enlarged, and vice versa. A reduction of the strength of the pion tensor force with respect to its experimental value from the nucleon-nucleon scattering is needed to get results closer to the experiment. Pairing correlations act to some extent in the opposite direction of the tensor term of the one-pion-exchange force.