QuantifyMe: An Open-Source Automated Single-Case Experimental Design Platform †

Science.gov (United States)

Sano, Akane; Ferguson, Craig; Mohan, Akshay; Picard, Rosalind W.

2018-01-01

Smartphones and wearable sensors have enabled unprecedented data collection, with many products now providing feedback to users about recommended step counts or sleep durations. However, these recommendations do not provide personalized insights that have been shown to be best suited for a specific individual. A scientific way to find individualized recommendations and causal links is to conduct experiments using single-case experimental design; however, properly designed single-case experiments are not easy to conduct on oneself. We designed, developed, and evaluated a novel platform, QuantifyMe, for novice self-experimenters to conduct proper-methodology single-case self-experiments in an automated and scientific manner using their smartphones. We provide software for the platform that we used (available for free on GitHub), which provides the methodological elements to run many kinds of customized studies. In this work, we evaluate its use with four different kinds of personalized investigations, examining how variables such as sleep duration and regularity, activity, and leisure time affect personal happiness, stress, productivity, and sleep efficiency. We conducted a six-week pilot study (N = 13) to evaluate QuantifyMe. We describe the lessons learned developing the platform and recommendations for its improvement, as well as its potential for enabling personalized insights to be scientifically evaluated in many individuals, reducing the high administrative cost for advancing human health and wellbeing. PMID:29621133

Implementing EW Receivers Based on Large Point Reconfigured FFT on FPGA Platforms

Directory of Open Access Journals (Sweden)

He Chen

2011-12-01

Full Text Available This paper presents design and implementation of digital receiver based on large point fast Fourier transform (FFT suitable for electronic warfare (EW applications. When implementing the FFT algorithm on field-programmable gate array (FPGA platforms, the primary goal is to maximize throughput and minimize area. This algorithm adopts two-dimension, parallel and pipeline stream mode and implements the reconfiguration of FFT's points. Moreover, a double-sequence-separation FFT algorithm has been implemented in order to achieve faster real time processing in broadband digital receivers. The performance of the hardware implementation on the FPGA platforms of broadband digital receivers has been analyzed in depth. It reaches the requirement of high-speed digital signal processing, and reveals the designing this kind of digital signal processing systems on FPGA platforms. Keywords: digital receivers, field programmable gate array (FPGA, fast Fourier transform (FFT, large point reconfigured, signal processing system.

Low consumption single-use microvalve for microfluidic PCB-based platforms

International Nuclear Information System (INIS)

Flores, G; Aracil, C; Perdigones, F; Quero, J M

2014-01-01

In this paper, a single-use and unidirectional microvalve with low consumption of energy for PCB-based microfluidic platforms is reported. Its activation is easy because it works as a fuse. The fabrication process of the device is based on PCB technology and a typical SU-8 process, using the PCB as a substrate and SU-8 for the microfluidic channels and chambers. The microvalve is intended to be used to impulse small volumes of fluids and it has been designed to be highly integrable in PCB-based microfluidic platforms. The proposed device has been fabricated, integrated and tested in a general purpose microfluidic circuit, resulting in a low activation time, of about 100 μs, and a low consumption of energy, with a maximum of 27 mJ. These results show a significant improvement because the energy consumption is about 84% lower and the time response is about four orders of magnitude shorter if compared with similar microvalves for impulsion of fluids on PCB-based platforms. (paper)

Single-Chip Multiple-Frequency RF MEMS Resonant Platform for Wireless Communications, Phase I

Data.gov (United States)

National Aeronautics and Space Administration — A novel, single-chip, multiple-frequency platform for RF/IF filtering and clock reference based on contour-mode aluminum nitride (AlN) MEMS piezoelectric resonators...

Microfluidic platform for multiplexed detection in single cells and methods thereof

Science.gov (United States)

Wu, Meiye; Singh, Anup K.

2018-05-01

The present invention relates to a microfluidic device and platform configured to conduct multiplexed analysis within the device. In particular, the device allows multiple targets to be detected on a single-cell level. Also provided are methods of performing multiplexed analyses to detect one or more target nucleic acids, proteins, and post-translational modifications.

Evaluating Unmanned Aerial Platforms for Cultural Heritage Large Scale Mapping

Science.gov (United States)

Georgopoulos, A.; Oikonomou, C.; Adamopoulos, E.; Stathopoulou, E. K.

2016-06-01

When it comes to large scale mapping of limited areas especially for cultural heritage sites, things become critical. Optical and non-optical sensors are developed to such sizes and weights that can be lifted by such platforms, like e.g. LiDAR units. At the same time there is an increase in emphasis on solutions that enable users to get access to 3D information faster and cheaper. Considering the multitude of platforms, cameras and the advancement of algorithms in conjunction with the increase of available computing power this challenge should and indeed is further investigated. In this paper a short review of the UAS technologies today is attempted. A discussion follows as to their applicability and advantages, depending on their specifications, which vary immensely. The on-board cameras available are also compared and evaluated for large scale mapping. Furthermore a thorough analysis, review and experimentation with different software implementations of Structure from Motion and Multiple View Stereo algorithms, able to process such dense and mostly unordered sequence of digital images is also conducted and presented. As test data set, we use a rich optical and thermal data set from both fixed wing and multi-rotor platforms over an archaeological excavation with adverse height variations and using different cameras. Dense 3D point clouds, digital terrain models and orthophotos have been produced and evaluated for their radiometric as well as metric qualities.

A new large-scale manufacturing platform for complex biopharmaceuticals.

Science.gov (United States)

Vogel, Jens H; Nguyen, Huong; Giovannini, Roberto; Ignowski, Jolene; Garger, Steve; Salgotra, Anil; Tom, Jennifer

2012-12-01

Complex biopharmaceuticals, such as recombinant blood coagulation factors, are addressing critical medical needs and represent a growing multibillion-dollar market. For commercial manufacturing of such, sometimes inherently unstable, molecules it is important to minimize product residence time in non-ideal milieu in order to obtain acceptable yields and consistently high product quality. Continuous perfusion cell culture allows minimization of residence time in the bioreactor, but also brings unique challenges in product recovery, which requires innovative solutions. In order to maximize yield, process efficiency, facility and equipment utilization, we have developed, scaled-up and successfully implemented a new integrated manufacturing platform in commercial scale. This platform consists of a (semi-)continuous cell separation process based on a disposable flow path and integrated with the upstream perfusion operation, followed by membrane chromatography on large-scale adsorber capsules in rapid cycling mode. Implementation of the platform at commercial scale for a new product candidate led to a yield improvement of 40% compared to the conventional process technology, while product quality has been shown to be more consistently high. Over 1,000,000 L of cell culture harvest have been processed with 100% success rate to date, demonstrating the robustness of the new platform process in GMP manufacturing. While membrane chromatography is well established for polishing in flow-through mode, this is its first commercial-scale application for bind/elute chromatography in the biopharmaceutical industry and demonstrates its potential in particular for manufacturing of potent, low-dose biopharmaceuticals. Copyright © 2012 Wiley Periodicals, Inc.

Morphological indicators of growth stages in carbonates platform evolution: comparison between present-day and Miocene platforms of Northern Borneo, Malaysia.

Science.gov (United States)

Pierson, B.; Menier, D.; Ting, K. K.; Chalabi, A.

2012-04-01

Satellite images of present-day reefs and carbonate platforms of the Celebes Sea, east of Sabah, Malaysia, exhibit large-scale features indicative of the recent evolution of the platforms. These include: (1) multiple, sub-parallel reef rims at the windward margin, suggestive of back-stepping of the platform margin; (2) contraction of the platform, possibly as a result of recent sea level fluctuations; (3) colonization of the internal lagoons by polygonal reef structures and (4) fragmentation of the platforms and creation of deep channels separating platforms that used to be part of a single entity. These features are analogue to what has been observed on seismic attribute maps of Miocene carbonate platforms of Sarawak. An analysis of several growth stages of a large Miocene platform, referred to as the Megaplatform, shows that the platform evolves in function of syn-depositional tectonic movements and sea level fluctuations that result in back-stepping of the margin, illustrated by multiple reef rims, contraction of the platform, the development of polygonal structures currently interpreted as karstic in origin and fragmentation of the megaplatform in 3 sub-entities separated by deep channels that precedes the final demise of the whole platform. Comparing similar features on present-day to platforms and Miocene platforms leads to a better understanding of the growth history of Miocene platforms and to a refined predictability of reservoir and non-reservoir facies distribution.

A novel robotic platform for single-port abdominal surgery

Science.gov (United States)

Singh, Satwinder; Cheung, Jo L. K.; Sreedhar, Biji; Hoa, Xuyen Dai; Ng, Hoi Pang; Yeung, Chung Kwong

2018-03-01

In this paper, a novel robot-assisted platform for single-port minimally invasive surgery is presented. A miniaturized seven degrees of freedom (dof) fully internalized in-vivo actuated robotic arm is designed. Due to in-vivo actuation, the system has a smaller footprint and can generate 20 N of gripping force. The complete work envelop of the robotic arms is 252 mm × 192 mm × 322 m. With the assistance of the cannula-swivel system, the robotic arms can also be re-positioned and have multi-quadrant reachability without any additional incision. Surgical tasks, such as lifting, gripping suturing and knot tying that are commonly used in a standard surgical procedure, were performed to verify the dexterity of the robotic arms. A single-port trans-abdominal cholecystectomy in a porcine model was successfully performed to further validate its functionality.

The Oncopig Cancer Model: An Innovative Large Animal Translational Oncology Platform

DEFF Research Database (Denmark)

Schachtschneider, Kyle M.; Schwind, Regina M.; Newson, Jordan

2017-01-01

-the Oncopig Cancer Model (OCM)-as a next-generation large animal platform for the study of hematologic and solid tumor oncology. With mutations in key tumor suppressor and oncogenes, TP53R167H and KRASG12D , the OCM recapitulates transcriptional hallmarks of human disease while also exhibiting clinically...

Initial experience with the new da Vinci single-port robot-assisted platform.

Science.gov (United States)

Ballestero Diego, R; Zubillaga Guerrero, S; Truan Cacho, D; Carrion Ballardo, C; Velilla Diez, G; Calleja Hermosa, P; Gutiérrez Baños, J L

2017-06-01

To describe our experience in the first cases of urological surgeries performed with the da Vinci single-port robot-assisted platform. We performed 5 single-port robot-assisted surgeries (R-LESS) between May and October 2014. We performed 3 ureteral reimplant surgeries, one ureteropyeloplasty in an inverted kidney and 1 partial nephrectomy. The perioperative and postoperative results were collected, as well as a report of the complications according to the Clavien classification system. Of the 5 procedures, 4 were performed completely by LESS, while 1 procedure was reconverted to multiport robot-assisted surgery. There were no intraoperative complications. We observed perioperative complications in 4 patients, all of which were grade 1 or 2. The mean surgical time was 262minutes (range, 230-300). In our initial experience with the da Vinci device, R-LESS surgery was feasible and safe. There are still a number of limitations in its use, which require new and improved R-LESS platforms. Copyright © 2016 AEU. Publicado por Elsevier España, S.L.U. All rights reserved.

Optically-controlled platforms for transfection and single- and sub-cellular surgery

DEFF Research Database (Denmark)

Villangca, Mark Jayson; Casey, Duncan; Glückstad, Jesper

2015-01-01

and specificity of optical trapping in conjunction with other modalities to perform single and sub-cellular surgery. These tools form highly tuneable platforms for the delivery or removal of material from cells of interest, but can simultaneously excite fluorescent probes for imaging purposes or plasmonic...... structures for very local heating. We discuss both the history and recent applications of the field, highlighting the key findings and developments over the last 40 years of biophotonics research....

Consumers Intention to Use a Single Platform E-Payment System: A Study Among Malaysian Internet and Mobile Banking Users

OpenAIRE

LAI POEY CHIN; ZAINAL ARFFIN AHMAD

2015-01-01

This study seeks to explore Malaysian Internet and Mobile banking consumers’ in adopting a single platform E-payment system using TAM model. This research seeks to investigate whether convenience and design influence perceived ease of use and perceived usefulness. We also investigated the relationship between perceived ease of use, perceived usefulness and perceived risk with consumers’ intention to use one single platform that integrates card, internet and mobile. Respondents who used both t...

mmpdb: An Open-Source Matched Molecular Pair Platform for Large Multiproperty Data Sets.

Science.gov (United States)

Dalke, Andrew; Hert, Jérôme; Kramer, Christian

2018-05-29

Matched molecular pair analysis (MMPA) enables the automated and systematic compilation of medicinal chemistry rules from compound/property data sets. Here we present mmpdb, an open-source matched molecular pair (MMP) platform to create, compile, store, retrieve, and use MMP rules. mmpdb is suitable for the large data sets typically found in pharmaceutical and agrochemical companies and provides new algorithms for fragment canonicalization and stereochemistry handling. The platform is written in Python and based on the RDKit toolkit. It is freely available from https://github.com/rdkit/mmpdb .

Multistage stereotactic radiosurgery for large cerebral arteriovenous malformations using the Gamma Knife platform.

Science.gov (United States)

Ding, Chuxiong; Hrycushko, Brian; Whitworth, Louis; Li, Xiang; Nedzi, Lucien; Weprin, Bradley; Abdulrahman, Ramzi; Welch, Babu; Jiang, Steve B; Wardak, Zabi; Timmerman, Robert D

2017-10-01

Radiosurgery is an established technique to treat cerebral arteriovenous malformations (AVMs). Obliteration of larger AVMs (> 10-15 cm 3 or diameter > 3 cm) in a single session is challenging with current radiosurgery platforms due to toxicity. We present a novel technique of multistage stereotactic radiosurgery (SRS) for large intracranial arteriovenous malformations (AVM) using the Gamma Knife system. Eighteen patients with large (> 10-15 cm 3 or diameter > 3 cm) AVMs, which were previously treated using a staged SRS technique on the Cyberknife platform, were retrospectively selected for this study. The AVMs were contoured and divided into 3-8 subtargets to be treated sequentially in a staged approach at half to 4 week intervals. The prescription dose ranged from 15 Gy to 20 Gy, depending on the subtarget number, volume, and location. Gamma Knife plans using multiple collimator settings were generated and optimized. The coordinates of each shot from the initial plan covering the total AVM target were extracted based on their relative positions within the frame system. The shots were regrouped based on their location with respect to the subtarget contours to generate subplans for each stage. The delivery time of each shot for a subtarget was decay corrected with 60 Co for staging the treatment course to generate the same dose distribution as that planned for the total AVM target. Conformality indices and dose-volume analysis were performed to evaluate treatment plans. With the shot redistribution technique, the composite dose for the multistaged treatment of multiple subtargets is equivalent to the initial plan for total AVM target. Gamma Knife plans resulted in an average PTV coverage of 96.3 ± 0.9% and a PITV of 1.23 ± 0.1. The resulting Conformality indices, V 12Gy and R 50 dose spillage values were 0.76 ± 0.05, 3.4 ± 1.8, and 3.1 ± 0.5 respectively. The Gamma Knife system can deliver a multistaged conformal dose to treat large AVMs when correcting for

Cloud-based bioinformatics workflow platform for large-scale next-generation sequencing analyses.

Science.gov (United States)

Liu, Bo; Madduri, Ravi K; Sotomayor, Borja; Chard, Kyle; Lacinski, Lukasz; Dave, Utpal J; Li, Jianqiang; Liu, Chunchen; Foster, Ian T

2014-06-01

Due to the upcoming data deluge of genome data, the need for storing and processing large-scale genome data, easy access to biomedical analyses tools, efficient data sharing and retrieval has presented significant challenges. The variability in data volume results in variable computing and storage requirements, therefore biomedical researchers are pursuing more reliable, dynamic and convenient methods for conducting sequencing analyses. This paper proposes a Cloud-based bioinformatics workflow platform for large-scale next-generation sequencing analyses, which enables reliable and highly scalable execution of sequencing analyses workflows in a fully automated manner. Our platform extends the existing Galaxy workflow system by adding data management capabilities for transferring large quantities of data efficiently and reliably (via Globus Transfer), domain-specific analyses tools preconfigured for immediate use by researchers (via user-specific tools integration), automatic deployment on Cloud for on-demand resource allocation and pay-as-you-go pricing (via Globus Provision), a Cloud provisioning tool for auto-scaling (via HTCondor scheduler), and the support for validating the correctness of workflows (via semantic verification tools). Two bioinformatics workflow use cases as well as performance evaluation are presented to validate the feasibility of the proposed approach. Copyright © 2014 Elsevier Inc. All rights reserved.

RFID sensors as the common sensing platform for single-use biopharmaceutical manufacturing

International Nuclear Information System (INIS)

Potyrailo, Radislav A; Surman, Cheryl; Monk, David; Morris, William G; Wortley, Timothy; Vincent, Mark; Diana, Rafael; Pizzi, Vincent; Carter, Jeffrey; Gach, Gerard; Klensmeden, Staffan; Ehring, Hanno

2011-01-01

The lack of reliable single-use sensors prevents the biopharmaceutical industry from fully embracing single-use biomanufacturing processes. Sensors based on the same detection platform for all critical parameters in single-use bioprocess components would be highly desirable to significantly simplify their installation, calibration and operation. We review here our approach for passive radio-frequency identification (RFID)-based sensing that does not rely on costly proprietary RFID memory chips with an analog input but rather implements ubiquitous passive 13.56 MHz RFID tags as inductively coupled sensors with at least 16 bit resolution provided by a sensor reader. The developed RFID sensors combine several measured parameters from the resonant sensor antenna with multivariate data analysis and deliver unique capability of multiparameter sensing and rejection of environmental interferences with a single sensor. This general sensing approach provides an elegant solution for both analytical measurement and identification and documentation of the measured location. (topical review)

Large Scale Simulation Platform for NODES Validation Study

Energy Technology Data Exchange (ETDEWEB)

Sotorrio, P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Qin, Y. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Min, L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2017-04-27

This report summarizes the Large Scale (LS) simulation platform created for the Eaton NODES project. The simulation environment consists of both wholesale market simulator and distribution simulator and includes the CAISO wholesale market model and a PG&E footprint of 25-75 feeders to validate the scalability under a scenario of 33% RPS in California with additional 17% of DERS coming from distribution and customers. The simulator can generate hourly unit commitment, 5-minute economic dispatch, and 4-second AGC regulation signals. The simulator is also capable of simulating greater than 10k individual controllable devices. Simulated DERs include water heaters, EVs, residential and light commercial HVAC/buildings, and residential-level battery storage. Feeder-level voltage regulators and capacitor banks are also simulated for feeder-level real and reactive power management and Vol/Var control.

Ultra-large single crystals by abnormal grain growth.

Science.gov (United States)

Kusama, Tomoe; Omori, Toshihiro; Saito, Takashi; Kise, Sumio; Tanaka, Toyonobu; Araki, Yoshikazu; Kainuma, Ryosuke

2017-08-25

Producing a single crystal is expensive because of low mass productivity. Therefore, many metallic materials are being used in polycrystalline form, even though material properties are superior in a single crystal. Here we show that an extraordinarily large Cu-Al-Mn single crystal can be obtained by abnormal grain growth (AGG) induced by simple heat treatment with high mass productivity. In AGG, the sub-boundary energy introduced by cyclic heat treatment (CHT) is dominant in the driving pressure, and the grain boundary migration rate is accelerated by repeating the low-temperature CHT due to the increase of the sub-boundary energy. With such treatment, fabrication of single crystal bars 70 cm in length is achieved. This result ensures that the range of applications of shape memory alloys will spread beyond small-sized devices to large-scale components and may enable new applications of single crystals in other metallic and ceramics materials having similar microstructural features.Growing large single crystals cheaply and reliably for structural applications remains challenging. Here, the authors combine accelerated abnormal grain growth and cyclic heat treatments to grow a superelastic shape memory alloy single crystal to 70 cm.

Multi-platform Integrated Positioning and Attitude Determination using GNSS

NARCIS (Netherlands)

Buist, P.J.

2013-01-01

There is trend in spacecraft engineering toward distributed systems where a number of smaller spacecraft work as a larger satellite. However, in order to make the small satellites work together as a single large platform, the precise relative positions (baseline) and orientations (attitude) of the

Rectangular coordination polymer nanoplates: large-scale, rapid synthesis and their application as a fluorescent sensing platform for DNA detection.

Directory of Open Access Journals (Sweden)

Yingwei Zhang

Full Text Available In this paper, we report on the large-scale, rapid synthesis of uniform rectangular coordination polymer nanoplates (RCPNs assembled from Cu(II and 4,4'-bipyridine for the first time. We further demonstrate that such RCPNs can be used as a very effective fluorescent sensing platform for multiple DNA detection with a detection limit as low as 30 pM and a high selectivity down to single-base mismatch. The DNA detection is accomplished by the following two steps: (1 RCPN binds dye-labeled single-stranded DNA (ssDNA probe, which brings dye and RCPN into close proximity, leading to fluorescence quenching; (2 Specific hybridization of the probe with its target generates a double-stranded DNA (dsDNA which detaches from RCPN, leading to fluorescence recovery. It suggests that this sensing system can well discriminate complementary and mismatched DNA sequences. The exact mechanism of fluorescence quenching involved is elucidated experimentally and its use in a human blood serum system is also demonstrated successfully.

GATECloud.net: a platform for large-scale, open-source text processing on the cloud.

Science.gov (United States)

Tablan, Valentin; Roberts, Ian; Cunningham, Hamish; Bontcheva, Kalina

2013-01-28

Cloud computing is increasingly being regarded as a key enabler of the 'democratization of science', because on-demand, highly scalable cloud computing facilities enable researchers anywhere to carry out data-intensive experiments. In the context of natural language processing (NLP), algorithms tend to be complex, which makes their parallelization and deployment on cloud platforms a non-trivial task. This study presents a new, unique, cloud-based platform for large-scale NLP research--GATECloud. net. It enables researchers to carry out data-intensive NLP experiments by harnessing the vast, on-demand compute power of the Amazon cloud. Important infrastructural issues are dealt with by the platform, completely transparently for the researcher: load balancing, efficient data upload and storage, deployment on the virtual machines, security and fault tolerance. We also include a cost-benefit analysis and usage evaluation.

Robot Work Platform for Large Hot Cell Deactivation

International Nuclear Information System (INIS)

BITTEN, E.J.

2000-01-01

The 324 Building, located at the Hanford Site near Richland, Washington, is being deactivated to meet state and federal cleanup commitments. The facility is currently in its third year of a nine-year project to complete deactivation and closure for long-term surveillance and maintenance. The 324 building contains large hot cells that were used for high-radiation, high-contamination chemical process development and demonstrations. A major obstacle for the 324 deactivation project is the inability to effectively perform deactivation tasks within highly radioactive, contaminated environments. Current strategies use inefficient, resource intensive technologies that significantly impact the cost and schedule for deactivation. To meet mandated cleanup commitments, there is a need to deploy rapid, more efficient remote/robot technologies to minimize worker exposure, accelerate work tasks, and eliminate the need for multiple specialized tool design and procurement efforts. This paper describes the functions and performance requirements for a crane-deployed remote/robot Work Platform possessing full access capabilities. The remote/robot Work Platform will deploy commercially available off-the-shelf tools and end effectors to support Project cleanup goals and reduce overall project risk and cost. The intent of this system is to maximize the use of off-the-shelf technologies that minimize additional new, unproven, or novel designs. This paper further describes procurement strategy, the selection process, the selected technology, and the current status of the procurement and lessons learned. Funding, in part, has been provided by the US Department of Energy, Office of Science and Technology, Deactivation and Decommissioning Focus Area

A platform for high-throughput bioenergy production phenotype characterization in single cells

Science.gov (United States)

Kelbauskas, Laimonas; Glenn, Honor; Anderson, Clifford; Messner, Jacob; Lee, Kristen B.; Song, Ganquan; Houkal, Jeff; Su, Fengyu; Zhang, Liqiang; Tian, Yanqing; Wang, Hong; Bussey, Kimberly; Johnson, Roger H.; Meldrum, Deirdre R.

2017-01-01

Driven by an increasing number of studies demonstrating its relevance to a broad variety of disease states, the bioenergy production phenotype has been widely characterized at the bulk sample level. Its cell-to-cell variability, a key player associated with cancer cell survival and recurrence, however, remains poorly understood due to ensemble averaging of the current approaches. We present a technology platform for performing oxygen consumption and extracellular acidification measurements of several hundreds to 1,000 individual cells per assay, while offering simultaneous analysis of cellular communication effects on the energy production phenotype. The platform comprises two major components: a tandem optical sensor for combined oxygen and pH detection, and a microwell device for isolation and analysis of single and few cells in hermetically sealed sub-nanoliter chambers. Our approach revealed subpopulations of cells with aberrant energy production profiles and enables determination of cellular response variability to electron transfer chain inhibitors and ion uncouplers. PMID:28349963

Comparison of reverse transcription-quantitative polymerase chain reaction methods and platforms for single cell gene expression analysis.

Science.gov (United States)

Fox, Bridget C; Devonshire, Alison S; Baradez, Marc-Olivier; Marshall, Damian; Foy, Carole A

2012-08-15

Single cell gene expression analysis can provide insights into development and disease progression by profiling individual cellular responses as opposed to reporting the global average of a population. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the "gold standard" for the quantification of gene expression levels; however, the technical performance of kits and platforms aimed at single cell analysis has not been fully defined in terms of sensitivity and assay comparability. We compared three kits using purification columns (PicoPure) or direct lysis (CellsDirect and Cells-to-CT) combined with a one- or two-step RT-qPCR approach using dilutions of cells and RNA standards to the single cell level. Single cell-level messenger RNA (mRNA) analysis was possible using all three methods, although the precision, linearity, and effect of lysis buffer and cell background differed depending on the approach used. The impact of using a microfluidic qPCR platform versus a standard instrument was investigated for potential variability introduced by preamplification of template or scaling down of the qPCR to nanoliter volumes using laser-dissected single cell samples. The two approaches were found to be comparable. These studies show that accurate gene expression analysis is achievable at the single cell level and highlight the importance of well-validated experimental procedures for low-level mRNA analysis. Copyright © 2012 Elsevier Inc. All rights reserved.

Integrated numerical platforms for environmental dose assessments of large tritium inventory facilities

International Nuclear Information System (INIS)

Castro, P.; Ardao, J.; Velarde, M.; Sedano, L.; Xiberta, J.

2013-01-01

Related with a prospected new scenario of large inventory tritium facilities [KATRIN at TLK, CANDUs, ITER, EAST, other coming] the prescribed dosimetric limits by ICRP-60 for tritium committed-doses are under discussion requiring, in parallel, to surmount the highly conservative assessments by increasing the refinement of dosimetric-assessments in many aspects. Precise Lagrangian-computations of dosimetric cloud-evolution after standardized (normal/incidental/SBO) tritium cloud emissions are today numerically open to the perfect match of real-time meteorological-data, and patterns data at diverse scales for prompt/early and chronic tritium dose assessments. The trends towards integrated-numerical-platforms for environmental-dose assessments of large tritium inventory facilities under development.

Prospects for the synthesis of large single-crystal diamonds

International Nuclear Information System (INIS)

Khmelnitskiy, R A

2015-01-01

The unique properties of diamond have stimulated the study of and search for its applications in many fields, including optics, optoelectronics, electronics, biology, and electrochemistry. Whereas chemical vapor deposition allows the growth of polycrystalline diamond plates more than 200 mm in diameter, most current diamond application technologies require large-size (25 mm and more) single-crystal diamond substrates or films suitable for the photolithography process. This is quite a challenge, because the largest diamond crystals currently available are 10 mm or less in size. This review examines three promising approaches to fabricating large-size diamond single crystals: growing large-size single crystals, the deposition of heteroepitaxial diamond films on single-crystal substrates, and the preparation of composite diamond substrates. (reviews of topical problems)

The TeraShake Computational Platform for Large-Scale Earthquake Simulations

Science.gov (United States)

Cui, Yifeng; Olsen, Kim; Chourasia, Amit; Moore, Reagan; Maechling, Philip; Jordan, Thomas

Geoscientific and computer science researchers with the Southern California Earthquake Center (SCEC) are conducting a large-scale, physics-based, computationally demanding earthquake system science research program with the goal of developing predictive models of earthquake processes. The computational demands of this program continue to increase rapidly as these researchers seek to perform physics-based numerical simulations of earthquake processes for larger meet the needs of this research program, a multiple-institution team coordinated by SCEC has integrated several scientific codes into a numerical modeling-based research tool we call the TeraShake computational platform (TSCP). A central component in the TSCP is a highly scalable earthquake wave propagation simulation program called the TeraShake anelastic wave propagation (TS-AWP) code. In this chapter, we describe how we extended an existing, stand-alone, wellvalidated, finite-difference, anelastic wave propagation modeling code into the highly scalable and widely used TS-AWP and then integrated this code into the TeraShake computational platform that provides end-to-end (initialization to analysis) research capabilities. We also describe the techniques used to enhance the TS-AWP parallel performance on TeraGrid supercomputers, as well as the TeraShake simulations phases including input preparation, run time, data archive management, and visualization. As a result of our efforts to improve its parallel efficiency, the TS-AWP has now shown highly efficient strong scaling on over 40K processors on IBM’s BlueGene/L Watson computer. In addition, the TSCP has developed into a computational system that is useful to many members of the SCEC community for performing large-scale earthquake simulations.

Systematic analysis of rocky shore platform morphology at large spatial scale using LiDAR-derived digital elevation models

Science.gov (United States)

Matsumoto, Hironori; Dickson, Mark E.; Masselink, Gerd

2017-06-01

Much of the existing research on rocky shore platforms describes results from carefully selected field sites, or comparisons between a relatively small number of selected sites. Here we describe a method to systematically analyse rocky shore morphology over a large area using LiDAR-derived digital elevation models. The method was applied to 700 km of coastline in southwest England; a region where there is considerable variation in wave climate and lithological settings, and a large alongshore variation in tidal range. Across-shore profiles were automatically extracted at 50 m intervals around the coast where information was available from the Coastal Channel Observatory coastal classification. Routines were developed to automatically remove non-platform profiles. The remaining 612 shore platform profiles were then subject to automated morphometric analyses, and correlation analysis in respect to three possible environmental controls: wave height, mean spring tidal range and rock strength. As expected, considerable scatter exists in the correlation analysis because only very coarse estimates of rock strength and wave height were applied, whereas variability in factors such as these can locally be the most important control on shoreline morphology. In view of this, it is somewhat surprising that overall consistency was found between previous published findings and the results from the systematic, automated analysis of LiDAR data: platform gradient increases as rock strength and tidal range increase, but decreases as wave height increases; platform width increases as wave height and tidal range increase, but decreases as rock strength increases. Previous studies have predicted shore platform gradient using tidal range alone. A multi-regression analysis of LiDAR data confirms that tidal range is the strongest predictor, but a new multi-factor empirical model considering tidal range, wave height, and rock strength yields better predictions of shore platform gradient

Next generation sensing platforms for extended deployments in large-scale, multidisciplinary, adaptive sampling and observational networks

Science.gov (United States)

Cross, J. N.; Meinig, C.; Mordy, C. W.; Lawrence-Slavas, N.; Cokelet, E. D.; Jenkins, R.; Tabisola, H. M.; Stabeno, P. J.

2016-12-01

New autonomous sensors have dramatically increased the resolution and accuracy of oceanographic data collection, enabling rapid sampling over extremely fine scales. Innovative new autonomous platofrms like floats, gliders, drones, and crawling moorings leverage the full potential of these new sensors by extending spatiotemporal reach across varied environments. During 2015 and 2016, The Innovative Technology for Arctic Exploration Program at the Pacific Marine Environmental Laboratory tested several new types of fully autonomous platforms with increased speed, durability, and power and payload capacity designed to deliver cutting-edge ecosystem assessment sensors to remote or inaccessible environments. The Expendable Ice-Tracking (EXIT) gloat developed by the NOAA Pacific Marine Environmental Laboratory (PMEL) is moored near bottom during the ice-free season and released on an autonomous timer beneath the ice during the following winter. The float collects a rapid profile during ascent, and continues to collect critical, poorly-accessible under-ice data until melt, when data is transmitted via satellite. The autonomous Oculus sub-surface glider developed by the University of Washington and PMEL has a large power and payload capacity and an enhanced buoyancy engine. This 'coastal truck' is designed for the rapid water column ascent required by optical imaging systems. The Saildrone is a solar and wind powered ocean unmanned surface vessel (USV) developed by Saildrone, Inc. in partnership with PMEL. This large-payload (200 lbs), fast (1-7 kts), durable (46 kts winds) platform was equipped with 15 sensors designed for ecosystem assessment during 2016, including passive and active acoustic systems specially redesigned for autonomous vehicle deployments. The senors deployed on these platforms achieved rigorous accuracy and precision standards. These innovative platforms provide new sampling capabilities and cost efficiencies in high-resolution sensor deployment

Snap: an integrated SNP annotation platform

DEFF Research Database (Denmark)

Li, Shengting; Ma, Lijia; Li, Heng

2007-01-01

Snap (Single Nucleotide Polymorphism Annotation Platform) is a server designed to comprehensively analyze single genes and relationships between genes basing on SNPs in the human genome. The aim of the platform is to facilitate the study of SNP finding and analysis within the framework of medical...

Robot-Assisted Laparoendoscopic Single-Site Partial Nephrectomy With the Novel Da Vinci Single-Site Platform: Initial Experience

Science.gov (United States)

Komninos, Christos; Tuliao, Patrick; Kim, Dae Keun; Choi, Young Deuk; Chung, Byung Ha

2014-01-01

Purpose To report our initial clinical cases of robotic laparoendoscopic single-site (R-LESS) partial nephrectomy (PN) performed with the use of the novel Da Vinci R-LESS platform. Materials and Methods Three patients underwent R-LESS PN from November 2013 through February 2014. Perioperative and postoperative outcomes were collected and intraoperative difficulties were noted. Results Operative time and estimated blood loss volume ranged between 100 and 110 minutes and between 50 and 500 mL, respectively. None of the patients was transfused. All cases were completed with the off-clamp technique, whereas one case required conversion to the conventional (multiport) approach because of difficulty in creating the appropriate scope for safe tumor resection. No major postoperative complications occurred, and all tumors were resected in safe margins. Length of hospital stay ranged between 3 and 7 days. The lack of EndoWrist movements, the external collisions, and the bed assistant's limited working space were noticed to be the main drawbacks of this surgical method. Conclusions Our initial experience with R-LESS PN with the novel Da Vinci platform shows that even though the procedure is feasible, it should be applied in only appropriately selected patients. However, further improvement is needed to overcome the existing limitations. PMID:24955221

istar: a web platform for large-scale protein-ligand docking.

Directory of Open Access Journals (Sweden)

Hongjian Li

Full Text Available Protein-ligand docking is a key computational method in the design of starting points for the drug discovery process. We are motivated by the desire to automate large-scale docking using our popular docking engine idock and thus have developed a publicly-accessible web platform called istar. Without tedious software installation, users can submit jobs using our website. Our istar website supports 1 filtering ligands by desired molecular properties and previewing the number of ligands to dock, 2 monitoring job progress in real time, and 3 visualizing ligand conformations and outputting free energy and ligand efficiency predicted by idock, binding affinity predicted by RF-Score, putative hydrogen bonds, and supplier information for easy purchase, three useful features commonly lacked on other online docking platforms like DOCK Blaster or iScreen. We have collected 17,224,424 ligands from the All Clean subset of the ZINC database, and revamped our docking engine idock to version 2.0, further improving docking speed and accuracy, and integrating RF-Score as an alternative rescoring function. To compare idock 2.0 with the state-of-the-art AutoDock Vina 1.1.2, we have carried out a rescoring benchmark and a redocking benchmark on the 2,897 and 343 protein-ligand complexes of PDBbind v2012 refined set and CSAR NRC HiQ Set 24Sept2010 respectively, and an execution time benchmark on 12 diverse proteins and 3,000 ligands of different molecular weight. Results show that, under various scenarios, idock achieves comparable success rates while outperforming AutoDock Vina in terms of docking speed by at least 8.69 times and at most 37.51 times. When evaluated on the PDBbind v2012 core set, our istar platform combining with RF-Score manages to reproduce Pearson's correlation coefficient and Spearman's correlation coefficient of as high as 0.855 and 0.859 respectively between the experimental binding affinity and the predicted binding affinity of the docked

istar: a web platform for large-scale protein-ligand docking.

Science.gov (United States)

Li, Hongjian; Leung, Kwong-Sak; Ballester, Pedro J; Wong, Man-Hon

2014-01-01

Protein-ligand docking is a key computational method in the design of starting points for the drug discovery process. We are motivated by the desire to automate large-scale docking using our popular docking engine idock and thus have developed a publicly-accessible web platform called istar. Without tedious software installation, users can submit jobs using our website. Our istar website supports 1) filtering ligands by desired molecular properties and previewing the number of ligands to dock, 2) monitoring job progress in real time, and 3) visualizing ligand conformations and outputting free energy and ligand efficiency predicted by idock, binding affinity predicted by RF-Score, putative hydrogen bonds, and supplier information for easy purchase, three useful features commonly lacked on other online docking platforms like DOCK Blaster or iScreen. We have collected 17,224,424 ligands from the All Clean subset of the ZINC database, and revamped our docking engine idock to version 2.0, further improving docking speed and accuracy, and integrating RF-Score as an alternative rescoring function. To compare idock 2.0 with the state-of-the-art AutoDock Vina 1.1.2, we have carried out a rescoring benchmark and a redocking benchmark on the 2,897 and 343 protein-ligand complexes of PDBbind v2012 refined set and CSAR NRC HiQ Set 24Sept2010 respectively, and an execution time benchmark on 12 diverse proteins and 3,000 ligands of different molecular weight. Results show that, under various scenarios, idock achieves comparable success rates while outperforming AutoDock Vina in terms of docking speed by at least 8.69 times and at most 37.51 times. When evaluated on the PDBbind v2012 core set, our istar platform combining with RF-Score manages to reproduce Pearson's correlation coefficient and Spearman's correlation coefficient of as high as 0.855 and 0.859 respectively between the experimental binding affinity and the predicted binding affinity of the docked conformation. istar

Multiple inflammatory biomarker detection in a prospective cohort study: a cross-validation between well-established single-biomarker techniques and an electrochemiluminescense-based multi-array platform.

Directory of Open Access Journals (Sweden)

Bas C T van Bussel

Full Text Available BACKGROUND: In terms of time, effort and quality, multiplex technology is an attractive alternative for well-established single-biomarker measurements in clinical studies. However, limited data comparing these methods are available. METHODS: We measured, in a large ongoing cohort study (n = 574, by means of both a 4-plex multi-array biomarker assay developed by MesoScaleDiscovery (MSD and single-biomarker techniques (ELISA or immunoturbidimetric assay, the following biomarkers of low-grade inflammation: C-reactive protein (CRP, serum amyloid A (SAA, soluble intercellular adhesion molecule 1 (sICAM-1 and soluble vascular cell adhesion molecule 1 (sVCAM-1. These measures were realigned by weighted Deming regression and compared across a wide spectrum of subjects' cardiovascular risk factors by ANOVA. RESULTS: Despite that both methods ranked individuals' levels of biomarkers very similarly (Pearson's r all≥0.755 absolute concentrations of all biomarkers differed significantly between methods. Equations retrieved by the Deming regression enabled proper realignment of the data to overcome these differences, such that intra-class correlation coefficients were then 0.996 (CRP, 0.711 (SAA, 0.895 (sICAM-1 and 0.858 (sVCAM-1. Additionally, individual biomarkers differed across categories of glucose metabolism, weight, metabolic syndrome and smoking status to a similar extent by either method. CONCLUSIONS: Multiple low-grade inflammatory biomarker data obtained by the 4-plex multi-array platform of MSD or by well-established single-biomarker methods are comparable after proper realignment of differences in absolute concentrations, and are equally associated with cardiovascular risk factors, regardless of such differences. Given its greater efficiency, the MSD platform is a potential tool for the quantification of multiple biomarkers of low-grade inflammation in large ongoing and future clinical studies.

Power in the loop real time simulation platform for renewable energy generation

Science.gov (United States)

Li, Yang; Shi, Wenhui; Zhang, Xing; He, Guoqing

2018-02-01

Nowadays, a large scale of renewable energy sources has been connecting to power system and the real time simulation platform is widely used to carry out research on integration control algorithm, power system stability etc. Compared to traditional pure digital simulation and hardware in the loop simulation, power in the loop simulation has higher accuracy and degree of reliability. In this paper, a power in the loop analog digital hybrid simulation platform has been built and it can be used not only for the single generation unit connecting to grid, but also for multiple new energy generation units connecting to grid. A wind generator inertia control experiment was carried out on the platform. The structure of the inertia control platform was researched and the results verify that the platform is up to need for renewable power in the loop real time simulation.

Seqcrawler: biological data indexing and browsing platform.

Science.gov (United States)

Sallou, Olivier; Bretaudeau, Anthony; Roult, Aurelien

2012-07-24

Seqcrawler takes its roots in software like SRS or Lucegene. It provides an indexing platform to ease the search of data and meta-data in biological banks and it can scale to face the current flow of data. While many biological bank search tools are available on the Internet, mainly provided by large organizations to search their data, there is a lack of free and open source solutions to browse one's own set of data with a flexible query system and able to scale from a single computer to a cloud system. A personal index platform will help labs and bioinformaticians to search their meta-data but also to build a larger information system with custom subsets of data. The software is scalable from a single computer to a cloud-based infrastructure. It has been successfully tested in a private cloud with 3 index shards (pieces of index) hosting ~400 millions of sequence information (whole GenBank, UniProt, PDB and others) for a total size of 600 GB in a fault tolerant architecture (high-availability). It has also been successfully integrated with software to add extra meta-data from blast results to enhance users' result analysis. Seqcrawler provides a complete open source search and store solution for labs or platforms needing to manage large amount of data/meta-data with a flexible and customizable web interface. All components (search engine, visualization and data storage), though independent, share a common and coherent data system that can be queried with a simple HTTP interface. The solution scales easily and can also provide a high availability infrastructure.

Seqcrawler: biological data indexing and browsing platform

Directory of Open Access Journals (Sweden)

Sallou Olivier

2012-07-01

Full Text Available Abstract Background Seqcrawler takes its roots in software like SRS or Lucegene. It provides an indexing platform to ease the search of data and meta-data in biological banks and it can scale to face the current flow of data. While many biological bank search tools are available on the Internet, mainly provided by large organizations to search their data, there is a lack of free and open source solutions to browse one’s own set of data with a flexible query system and able to scale from a single computer to a cloud system. A personal index platform will help labs and bioinformaticians to search their meta-data but also to build a larger information system with custom subsets of data. Results The software is scalable from a single computer to a cloud-based infrastructure. It has been successfully tested in a private cloud with 3 index shards (pieces of index hosting ~400 millions of sequence information (whole GenBank, UniProt, PDB and others for a total size of 600 GB in a fault tolerant architecture (high-availability. It has also been successfully integrated with software to add extra meta-data from blast results to enhance users’ result analysis. Conclusions Seqcrawler provides a complete open source search and store solution for labs or platforms needing to manage large amount of data/meta-data with a flexible and customizable web interface. All components (search engine, visualization and data storage, though independent, share a common and coherent data system that can be queried with a simple HTTP interface. The solution scales easily and can also provide a high availability infrastructure.

Corrected Lymphocyte Percentages Reduce the Differences in Absolute CD4+ T Lymphocyte Counts between Dual-Platform and Single-Platform Flow Cytometric Approaches.

Science.gov (United States)

Noulsri, Egarit; Abudaya, Dinar; Lerdwana, Surada; Pattanapanyasat, Kovit

2018-03-13

To determine whether a corrected lymphocyte percentage could reduce bias in the absolute cluster of differentiation (CD)4+ T lymphocyte counts obtained via dual-platform (DP) vs standard single-platform (SP) flow cytometry. The correction factor (CF) for the lymphocyte percentages was calculated at 6 laboratories. The absolute CD4+ T lymphocyte counts in 300 blood specimens infected with human immunodeficiency virus (HIV) were determined using the DP and SP methods. Applying the CFs revealed that 4 sites showed a decrease in the mean bias of absolute CD4+ T lymphocyte counts determined via DP vs standard SP (-109 vs -84 cells/μL, -80 vs -58 cells/μL, -52 vs -45 cells/μL, and -32 vs 1 cells/μL). However, 2 participating laboratories revealed an increase in the difference of the mean bias (-42 vs -49 cells/μL and -20 vs -69 cells/μL). Use of the corrected lymphocyte percentage shows potential for decreasing the difference in CD4 counts between DP and the standard SP method.

On-Chip Magnetic Platform for Single-Particle Manipulation with Integrated Electrical Feedback.

Science.gov (United States)

Monticelli, Marco; Torti, Andrea; Cantoni, Matteo; Petti, Daniela; Albisetti, Edoardo; Manzin, Alessandra; Guerriero, Erica; Sordan, Roman; Gervasoni, Giacomo; Carminati, Marco; Ferrari, Giorgio; Sampietro, Marco; Bertacco, Riccardo

2016-02-17

Methods for the manipulation of single magnetic particles have become very interesting, in particular for in vitro biological studies. Most of these studies require an external microscope to provide the operator with feedback for controlling the particle motion, thus preventing the use of magnetic particles in high-throughput experiments. In this paper, a simple and compact system with integrated electrical feedback is presented, implementing in the very same device both the manipulation and detection of the transit of single particles. The proposed platform is based on zig-zag shaped magnetic nanostructures, where transverse magnetic domain walls are pinned at the corners and attract magnetic particles in suspension. By applying suitable external magnetic fields, the domain walls move to the nearest corner, thus causing the step by step displacement of the particles along the nanostructure. The very same structure is also employed for detecting the bead transit. Indeed, the presence of the magnetic particle in suspension over the domain wall affects the depinning field required for its displacement. This characteristic field can be monitored through anisotropic magnetoresistance measurements, thus implementing an integrated electrical feedback of the bead transit. In particular, the individual manipulation and detection of single 1-μm sized beads is demonstrated. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Does transition from the da Vinci Si to Xi robotic platform impact single-docking technique for robot-assisted laparoscopic nephroureterectomy?

Science.gov (United States)

Patel, Manish N; Aboumohamed, Ahmed; Hemal, Ashok

2015-12-01

To describe our robot-assisted nephroureterectomy (RNU) technique for benign indications and RNU with en bloc excision of bladder cuff (BCE) and lymphadenectomy (LND) for malignant indications using the da Vinci Si and da Vinci Xi robotic platform, with its pros and cons. The port placement described for Si can be used for standard and S robotic systems. This is the first report in the literature on the use of the da Vinci Xi robotic platform for RNU. After a substantial experience of RNU using different da Vinci robots from the standard to the Si platform in a single-docking fashion for benign and malignant conditions, we started using the newly released da Vinci Xi robot since 2014. The most important differences are in port placement and effective use of the features of da Vinci Xi robot while performing simultaneous upper and lower tract surgery. Patient positioning, port placement, step-by-step technique of single docking RNU-LND-BCE using the da Vinci Si and da Vinci Xi robot are shown in an accompanying video with the goal that centres using either robotic system benefit from the hints and tips. The first segment of video describes RNU-LND-BCE using the da Vinci Si followed by the da Vinci Xi to highlight differences. There was no need for patient repositioning or robot re-docking with the new da Vinci Xi robotic platform. We have experience of using different robotic systems for single docking RNU in 70 cases for benign (15) and malignant (55) conditions. The da Vinci Xi robotic platform helps operating room personnel in its easy movement, allows easier patient side-docking with the help of its boom feature, in addition to easy and swift movements of the robotic arms. The patient clearance feature can be used to avoid collision with the robotic arms or the patient's body. In patients with challenging body habitus and in situations where bladder cuff management is difficult, modifications can be made through reassigning the camera to a different port with

Hierarchical approach to optimization of parallel matrix multiplication on large-scale platforms

KAUST Repository

Hasanov, Khalid

2014-03-04

© 2014, Springer Science+Business Media New York. Many state-of-the-art parallel algorithms, which are widely used in scientific applications executed on high-end computing systems, were designed in the twentieth century with relatively small-scale parallelism in mind. Indeed, while in 1990s a system with few hundred cores was considered a powerful supercomputer, modern top supercomputers have millions of cores. In this paper, we present a hierarchical approach to optimization of message-passing parallel algorithms for execution on large-scale distributed-memory systems. The idea is to reduce the communication cost by introducing hierarchy and hence more parallelism in the communication scheme. We apply this approach to SUMMA, the state-of-the-art parallel algorithm for matrix–matrix multiplication, and demonstrate both theoretically and experimentally that the modified Hierarchical SUMMA significantly improves the communication cost and the overall performance on large-scale platforms.

Ensembles of indium phosphide nanowires: physical properties and functional devices integrated on non-single crystal platforms

International Nuclear Information System (INIS)

Kobayashi, Nobuhiko P.; Lohn, Andrew; Onishi, Takehiro; Mathai, Sagi; Li, Xuema; Straznicky, Joseph; Wang, Shih-Yuan; Williams, R.S.; Logeeswaran, V.J.; Islam, M.S.

2009-01-01

A new route to grow an ensemble of indium phosphide single-crystal semiconductor nanowires is described. Unlike conventional epitaxial growth of single-crystal semiconductor films, the proposed route for growing semiconductor nanowires does not require a single-crystal semiconductor substrate. In the proposed route, instead of using single-crystal semiconductor substrates that are characterized by their long-range atomic ordering, a template layer that possesses short-range atomic ordering prepared on a non-single-crystal substrate is employed. On the template layer, epitaxial information associated with its short-range atomic ordering is available within an area that is comparable to that of a nanowire root. Thus the template layer locally provides epitaxial information required for the growth of semiconductor nanowires. In the particular demonstration described in this paper, hydrogenated silicon was used as a template layer for epitaxial growth of indium phosphide nanowires. The indium phosphide nanowires grown on the hydrogenerated silicon template layer were found to be single crystal and optically active. Simple photoconductors and pin-diodes were fabricated and tested with the view towards various optoelectronic device applications where group III-V compound semiconductors are functionally integrated onto non-single-crystal platforms. (orig.)

Ensembles of indium phosphide nanowires: physical properties and functional devices integrated on non-single crystal platforms

Energy Technology Data Exchange (ETDEWEB)

Kobayashi, Nobuhiko P.; Lohn, Andrew; Onishi, Takehiro [University of California, Santa Cruz (United States). Baskin School of Engineering; NASA Ames Research Center, Nanostructured Energy Conversion Technology and Research (NECTAR), Advanced Studies Laboratories, Univ. of California Santa Cruz, Moffett Field, CA (United States); Mathai, Sagi; Li, Xuema; Straznicky, Joseph; Wang, Shih-Yuan; Williams, R.S. [Hewlett-Packard Laboratories, Information and Quantum Systems Laboratory, Palo Alto, CA (United States); Logeeswaran, V.J.; Islam, M.S. [University of California Davis, Electrical and Computer Engineering, Davis, CA (United States)

2009-06-15

A new route to grow an ensemble of indium phosphide single-crystal semiconductor nanowires is described. Unlike conventional epitaxial growth of single-crystal semiconductor films, the proposed route for growing semiconductor nanowires does not require a single-crystal semiconductor substrate. In the proposed route, instead of using single-crystal semiconductor substrates that are characterized by their long-range atomic ordering, a template layer that possesses short-range atomic ordering prepared on a non-single-crystal substrate is employed. On the template layer, epitaxial information associated with its short-range atomic ordering is available within an area that is comparable to that of a nanowire root. Thus the template layer locally provides epitaxial information required for the growth of semiconductor nanowires. In the particular demonstration described in this paper, hydrogenated silicon was used as a template layer for epitaxial growth of indium phosphide nanowires. The indium phosphide nanowires grown on the hydrogenerated silicon template layer were found to be single crystal and optically active. Simple photoconductors and pin-diodes were fabricated and tested with the view towards various optoelectronic device applications where group III-V compound semiconductors are functionally integrated onto non-single-crystal platforms. (orig.)

A Versatile Microarray Platform for Capturing Rare Cells

Science.gov (United States)

Brinkmann, Falko; Hirtz, Michael; Haller, Anna; Gorges, Tobias M.; Vellekoop, Michael J.; Riethdorf, Sabine; Müller, Volkmar; Pantel, Klaus; Fuchs, Harald

2015-10-01

Analyses of rare events occurring at extremely low frequencies in body fluids are still challenging. We established a versatile microarray-based platform able to capture single target cells from large background populations. As use case we chose the challenging application of detecting circulating tumor cells (CTCs) - about one cell in a billion normal blood cells. After incubation with an antibody cocktail, targeted cells are extracted on a microarray in a microfluidic chip. The accessibility of our platform allows for subsequent recovery of targets for further analysis. The microarray facilitates exclusion of false positive capture events by co-localization allowing for detection without fluorescent labelling. Analyzing blood samples from cancer patients with our platform reached and partly outreached gold standard performance, demonstrating feasibility for clinical application. Clinical researchers free choice of antibody cocktail without need for altered chip manufacturing or incubation protocol, allows virtual arbitrary targeting of capture species and therefore wide spread applications in biomedical sciences.

EURESCOM Services Platform

NARCIS (Netherlands)

Nieuwenhuis, Lambertus Johannes Maria; van Halteren, Aart

1999-01-01

This paper presents the results of the EURESCOM Project 715. In February 1999, a large team of researchers from six European public network operators completed a two year period of cooperative experiments on a TINA-based environment, called the EURESCOM Services Platform (ESP). This platform

A Comparison and Integration of MiSeq and MinION Platforms for Sequencing Single Source and Mixed Mitochondrial Genomes.

Directory of Open Access Journals (Sweden)

Michael R Lindberg

Full Text Available Single source and multiple donor (mixed samples of human mitochondrial DNA were analyzed and compared using the MinION and the MiSeq platforms. A generalized variant detection strategy was employed to provide a cursory framework for evaluating the reliability and accuracy of mitochondrial sequences produced by the MinION. The feasibility of long-read phasing was investigated to establish its efficacy in quantitatively distinguishing and deconvolving individuals in a mixture. Finally, a proof-of-concept was demonstrated by integrating both platforms in a hybrid assembly that leverages solely mixture data to accurately reconstruct full mitochondrial genomes.

ADMS Evaluation Platform

Energy Technology Data Exchange (ETDEWEB)

2018-01-23

Deploying an ADMS or looking to optimize its value? NREL offers a low-cost, low-risk evaluation platform for assessing ADMS performance. The National Renewable Energy Laboratory (NREL) has developed a vendor-neutral advanced distribution management system (ADMS) evaluation platform and is expanding its capabilities. The platform uses actual grid-scale hardware, large-scale distribution system models, and advanced visualization to simulate realworld conditions for the most accurate ADMS evaluation and experimentation.

Single-phase ProtoDUNE, the Prototype of a Single-Phase Liquid Argon TPC for DUNE at the CERN Neutrino Platform

CERN Document Server

Cavanna, F; Touramanis, C

2017-01-01

ProtoDUNE-SP is the single-phase DUNE Far Detector prototype that is under construction and will be operated at the CERN Neutrino Platform (NP) starting in 2018. It was proposed to the CERN SPSC in June 2015 (SPSC-P-351) and was approved in December 2015 as experiment NP04 (ProtoDUNE). ProtoDUNE-SP, a crucial part of the DUNE effort towards the construction of the first DUNE 10-kt fiducial mass far detector module (17 kt total LAr mass), is a significant experiment in its own right. With a total liquid argon (LAr) mass of 0.77 kt, it represents the largest monolithic single phase LArTPC detector to be built to date. It is housed in an extension to the EHN1 hall in the North Area, where the CERN NP is providing a new dedicated charged-particle test beamline. ProtoDUNE-SP aims to take its first beam data before the LHC long shutdown (LS2) at the end of 2018. ProtoDUNE-SP prototypes the designs of most of the single-phase DUNE far detector module (DUNE-SP) components at a 1:1 scale, with an extrapolation of abo...

Computing platforms for software-defined radio

CERN Document Server

Nurmi, Jari; Isoaho, Jouni; Garzia, Fabio

2017-01-01

This book addresses Software-Defined Radio (SDR) baseband processing from the computer architecture point of view, providing a detailed exploration of different computing platforms by classifying different approaches, highlighting the common features related to SDR requirements and by showing pros and cons of the proposed solutions. Coverage includes architectures exploiting parallelism by extending single-processor environment (such as VLIW, SIMD, TTA approaches), multi-core platforms distributing the computation to either a homogeneous array or a set of specialized heterogeneous processors, and architectures exploiting fine-grained, coarse-grained, or hybrid reconfigurability. Describes a computer engineering approach to SDR baseband processing hardware; Discusses implementation of numerous compute-intensive signal processing algorithms on single and multicore platforms; Enables deep understanding of optimization techniques related to power and energy consumption of multicore platforms using several basic a...

Neuroethologic differences in sleep deprivation induced by the single- and multiple-platform methods

Directory of Open Access Journals (Sweden)

R. Medeiros

1998-05-01

Full Text Available It has been proposed that the multiple-platform method (MP for desynchronized sleep (DS deprivation eliminates the stress induced by social isolation and by the restriction of locomotion in the single-platform (SP method. MP, however, induces a higher increase in plasma corticosterone and ACTH levels than SP. Since deprivation is of heuristic value to identify the functional role of this state of sleep, the objective of the present study was to determine the behavioral differences exhibited by rats during sleep deprivation induced by these two methods. All behavioral patterns exhibited by a group of 7 albino male Wistar rats submitted to 4 days of sleep deprivation by the MP method (15 platforms, spaced 150 mm apart and by 7 other rats submitted to sleep deprivation by the SP method were recorded in order to elaborate an ethogram. The behavioral patterns were quantitated in 10 replications by naive observers using other groups of 7 rats each submitted to the same deprivation schedule. Each quantification session lasted 35 min and the behavioral patterns presented by each rat over a period of 5 min were counted. The results obtained were: a rats submitted to the MP method changed platforms at a mean rate of 2.62 ± 1.17 platforms h-1 animal-1; b the number of episodes of noninteractive waking patterns for the MP animals was significantly higher than that for SP animals (1077 vs 768; c additional episodes of waking patterns (26.9 ± 18.9 episodes/session were promoted by social interaction in MP animals; d the cumulative number of sleep episodes observed in the MP test (311 was significantly lower (chi-square test, 1 d.f., P<0.05 than that observed in the SP test (534; e rats submitted to the MP test did not show the well-known increase in ambulatory activity observed after the end of the SP test; f comparison of 6 MP and 6 SP rats showed a significantly shorter latency to the onset of DS in MP rats (7.8 ± 4.3 and 29.0 ± 25.0 min, respectively

Functionalization of single-walled carbon nanotubes with protein by click chemistry as sensing platform for sensitized electrochemical immunoassay

International Nuclear Information System (INIS)

Qi Honglan; Ling Chen; Huang Ru; Qiu Xiaoying; Shangguan Li; Gao Qiang; Zhang Chengxiao

2012-01-01

Highlights: ► Single-walled carbon nanotubes were functionalized with protein by click chemistry. ► The SWNTs conjugated with protein showed excellent dispersion in water and kept good bioacitvity. ► A competitive electrochemical immunoassay for the determination of anti-IgG was developed with high sensitivity and good stability. - Abstract: The application of the Cu(I)-catalyzed [3 + 2] Huisgen cycloaddition to the functionalization of single-walled carbon nanotubes (SWNTs) with the protein and the use of the artificial SWNTs as a sensing platform for sensitive immunoassay were reported. Covalent functionalization of azide decorated SWNTs with alkyne modified protein was firstly accomplished by the Cu(I)-catalyzed [3 + 2] Huisgen cycloaddition. FT-IR spectroscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy and transmission electron micrograph were used to characterize the protein-functionalized SWNTs. It was found that the SWNTs conjugated with the proteins showed excellent dispersion in water and kept good bioacitivity when immunoglobulin (IgG) and horseradish peroxidase (HRP) were chosen as model proteins. As a proof-of-concept, IgG-functionalized SWNTs were immobilized onto the surface of a glassy carbon electrode by simple casting method as immunosensing platform and a sensitive competitive electrochemical immunoassay was developed for the determination of anti-immunoglobulin (anti-IgG) using HRP as enzyme label. The fabrication of the immunosensor were characterized by cyclic voltammetry and electrochemical impedance spectroscopy with the redox probe [Fe(CN) 6 ] 3−/4− . The SWNTs as immobilization platform showed better sensitizing effect, a detection limit of 30 pg mL −1 (S/N = 3) was obtained for anti-IgG. The proposed strategy provided a stable immobilization method and sensitized recognition platform for analytes. This work demonstrated that the click coupling of SWNTs with protein was an effective

Product Platform Screening at LEGO

DEFF Research Database (Denmark)

Mortensen, Niels Henrik; Steen Jensen, Thomas; Nielsen, Ole Fiil

2012-01-01

Product platforms offer great benefits to companies developing new products in highly competitive markets. Literature describes how a single platform can be designed from a technical point of view, but rarely mentions how the process begins. How do companies identify possible platform candidates...... after a few changes had been applied to the initial process layout. This case study shows how companies must focus on a limited selection of simultaneous projects in order to keep focus. Primary stakeholders must be involved from the very beginning, and short presentations of the platform concepts...

Optimization of magnetic switches for single particle and cell transport

Energy Technology Data Exchange (ETDEWEB)

Abedini-Nassab, Roozbeh; Yellen, Benjamin B., E-mail: yellen@duke.edu [Department of Mechanical Engineering and Materials Science, Duke University, Box 90300 Hudson Hall, Durham, North Carolina 27708 (United States); Joint Institute, University of Michigan—Shanghai Jiao Tong University, Shanghai Jiao Tong University, Shanghai 200240 (China); Murdoch, David M. [Department of Medicine, Duke University, Durham, North Carolina 27708 (United States); Kim, CheolGi [Department of Emerging Materials Science, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 711-873 (Korea, Republic of)

2014-06-28

The ability to manipulate an ensemble of single particles and cells is a key aim of lab-on-a-chip research; however, the control mechanisms must be optimized for minimal power consumption to enable future large-scale implementation. Recently, we demonstrated a matter transport platform, which uses overlaid patterns of magnetic films and metallic current lines to control magnetic particles and magnetic-nanoparticle-labeled cells; however, we have made no prior attempts to optimize the device geometry and power consumption. Here, we provide an optimization analysis of particle-switching devices based on stochastic variation in the particle's size and magnetic content. These results are immediately applicable to the design of robust, multiplexed platforms capable of transporting, sorting, and storing single cells in large arrays with low power and high efficiency.

An Anomaly Detection Algorithm of Cloud Platform Based on Self-Organizing Maps

Directory of Open Access Journals (Sweden)

Jun Liu

2016-01-01

Full Text Available Virtual machines (VM on a Cloud platform can be influenced by a variety of factors which can lead to decreased performance and downtime, affecting the reliability of the Cloud platform. Traditional anomaly detection algorithms and strategies for Cloud platforms have some flaws in their accuracy of detection, detection speed, and adaptability. In this paper, a dynamic and adaptive anomaly detection algorithm based on Self-Organizing Maps (SOM for virtual machines is proposed. A unified modeling method based on SOM to detect the machine performance within the detection region is presented, which avoids the cost of modeling a single virtual machine and enhances the detection speed and reliability of large-scale virtual machines in Cloud platform. The important parameters that affect the modeling speed are optimized in the SOM process to significantly improve the accuracy of the SOM modeling and therefore the anomaly detection accuracy of the virtual machine.

Cross-platform learning: on the nature of children's learning from multiple media platforms.

Science.gov (United States)

Fisch, Shalom M

2013-01-01

It is increasingly common for an educational media project to span several media platforms (e.g., TV, Web, hands-on materials), assuming that the benefits of learning from multiple media extend beyond those gained from one medium alone. Yet research typically has investigated learning from a single medium in isolation. This paper reviews several recent studies to explore cross-platform learning (i.e., learning from combined use of multiple media platforms) and how such learning compares to learning from one medium. The paper discusses unique benefits of cross-platform learning, a theoretical mechanism to explain how these benefits might arise, and questions for future research in this emerging field. Copyright © 2013 Wiley Periodicals, Inc., A Wiley Company.

TDat: An Efficient Platform for Processing Petabyte-Scale Whole-Brain Volumetric Images.

Science.gov (United States)

Li, Yuxin; Gong, Hui; Yang, Xiaoquan; Yuan, Jing; Jiang, Tao; Li, Xiangning; Sun, Qingtao; Zhu, Dan; Wang, Zhenyu; Luo, Qingming; Li, Anan

2017-01-01

Three-dimensional imaging of whole mammalian brains at single-neuron resolution has generated terabyte (TB)- and even petabyte (PB)-sized datasets. Due to their size, processing these massive image datasets can be hindered by the computer hardware and software typically found in biological laboratories. To fill this gap, we have developed an efficient platform named TDat, which adopts a novel data reformatting strategy by reading cuboid data and employing parallel computing. In data reformatting, TDat is more efficient than any other software. In data accessing, we adopted parallelization to fully explore the capability for data transmission in computers. We applied TDat in large-volume data rigid registration and neuron tracing in whole-brain data with single-neuron resolution, which has never been demonstrated in other studies. We also showed its compatibility with various computing platforms, image processing software and imaging systems.

Preparing for a Product Platform

DEFF Research Database (Denmark)

Fiil-Nielsen, Ole; Munk, Lone; Mortensen, Niels Henrik

2005-01-01

on commonalities and similarities in the product family, and variance should be based on customer demands. To relate these terms and to improve the basis on which decisions are made, we need a way of visualizing the hierarchy of the product family as well as the commonality and variance. This visualization method...... of the platform or ensuring that the platform can meet future demands will be very useful in the preparation process of a platform synthesis as well as in the updating or reengineering of an existing product development platform.......Experience in the industry as well as recent related scientific publications show the benefits of product development platforms. Companies use platforms to develop not a single but multiple products (i.e. a product family) simultaneously. When these product development projects are coordinated...

Large single-crystal diamond substrates for ionizing radiation detection

Energy Technology Data Exchange (ETDEWEB)

Girolami, Marco; Bellucci, Alessandro; Calvani, Paolo; Trucchi, Daniele M. [Istituto di Struttura della Materia (ISM), Consiglio Nazionale delle Ricerche (CNR), Sede Secondaria di Montelibretti, Monterotondo Stazione, Roma (Italy)

2016-10-15

The need for large active volume detectors for ionizing radiations and particles, with both large area and thickness, is becoming more and more compelling in a wide range of applications, spanning from X-ray dosimetry to neutron spectroscopy. Recently, 8.0 x 8.0 mm{sup 2} wide and 1.2 mm thick single-crystal diamond plates have been put on the market, representing a first step to the fabrication of large area monolithic diamond detectors with optimized charge transport properties, obtainable up to now only with smaller samples. The more-than-double thickness, if compared to standard plates (typically 500 μm thick), demonstrated to be effective in improving the detector response to highly penetrating ionizing radiations, such as γ-rays. Here we report on the first measurements performed on large active volume single-crystal diamond plates, both in the dark and under irradiation with optical wavelengths (190-1100 nm), X-rays, and radioactive γ-emitting sources ({sup 57}Co and {sup 22}Na). (copyright 2016 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

Strapdown Airborne Gravimetry Using a Combination of Commercial Software and Stable-Platform Gravity Estimates

DEFF Research Database (Denmark)

Jensen, Tim E.; Nielsen, J. Emil; Olesen, Arne V.

2017-01-01

into the long-wavelengths of the gravity estimates. This has made the stable-platform approach the preferred method for geodetic applications. In the summer of 2016, during a large airborne survey in Malaysia, a SIMU system was flown alongside a traditional LaCoste&Romberg (LCR) gravimeter. The SIMU......For the past two decades, airborne gravimetry using a Strapdown Inertial Measurement Unit (SIMU) has been producing gravity estimates comparable to the traditional stable-platform single-axis gravimeters. The challenge has been to control the long term drift of the IMU sensors, propagating...

A Web-based Distributed Voluntary Computing Platform for Large Scale Hydrological Computations

Science.gov (United States)

Demir, I.; Agliamzanov, R.

2014-12-01

Distributed volunteer computing can enable researchers and scientist to form large parallel computing environments to utilize the computing power of the millions of computers on the Internet, and use them towards running large scale environmental simulations and models to serve the common good of local communities and the world. Recent developments in web technologies and standards allow client-side scripting languages to run at speeds close to native application, and utilize the power of Graphics Processing Units (GPU). Using a client-side scripting language like JavaScript, we have developed an open distributed computing framework that makes it easy for researchers to write their own hydrologic models, and run them on volunteer computers. Users will easily enable their websites for visitors to volunteer sharing their computer resources to contribute running advanced hydrological models and simulations. Using a web-based system allows users to start volunteering their computational resources within seconds without installing any software. The framework distributes the model simulation to thousands of nodes in small spatial and computational sizes. A relational database system is utilized for managing data connections and queue management for the distributed computing nodes. In this paper, we present a web-based distributed volunteer computing platform to enable large scale hydrological simulations and model runs in an open and integrated environment.

Recent progress in large grain/single crystal high RRR niobium

International Nuclear Information System (INIS)

Ganapati Rao Myneni; Peter Kneisel; Tadeu Carneiro; S.R. Agnew; F. Stevie

2005-01-01

High RRR bulk niobium Superconducting Radio Frequency (SRF) cavity technology is chosen for the International Linear Collider (ILC). The SRF community was convinced until now that fine grain polycrystalline RRR niobium sheets obtained via forging and cross rolling are essential for forming the SRF Cavities. However, it was recently discovered under a joint Reference Metals Company, Inc., - JLAB CRADA that large grain/single crystal RRR niobium sliced directly from ingots is highly ductile reaching 100 percent elongation. This discovery led to the successful fabrication of several SRF single and/or multi cell structures, formed with sliced RRR discs from the ingots, operating at 2.3, 1.5 and 1.3 GHz. This new exciting development is expected to offer high performance accelerator structures not only at reduced costs but also with simpler fabrication and processing conditions. As a result there is a renewed interest in the evaluation and understanding of the large grain and single crystal niobium with respect to their mechanical and physical properties as well as the oxidation behavior and the influence of impurities such as hydrogen and Ta. In this paper the results of many collaborative studies on large grain and single crystal high RRR niobium between JLAB, Universities and Industry are presented

ASAP: a web-based platform for the analysis and interactive visualization of single-cell RNA-seq data.

Science.gov (United States)

Gardeux, Vincent; David, Fabrice P A; Shajkofci, Adrian; Schwalie, Petra C; Deplancke, Bart

2017-10-01

Single-cell RNA-sequencing (scRNA-seq) allows whole transcriptome profiling of thousands of individual cells, enabling the molecular exploration of tissues at the cellular level. Such analytical capacity is of great interest to many research groups in the world, yet these groups often lack the expertise to handle complex scRNA-seq datasets. We developed a fully integrated, web-based platform aimed at the complete analysis of scRNA-seq data post genome alignment: from the parsing, filtering and normalization of the input count data files, to the visual representation of the data, identification of cell clusters, differentially expressed genes (including cluster-specific marker genes), and functional gene set enrichment. This Automated Single-cell Analysis Pipeline (ASAP) combines a wide range of commonly used algorithms with sophisticated visualization tools. Compared with existing scRNA-seq analysis platforms, researchers (including those lacking computational expertise) are able to interact with the data in a straightforward fashion and in real time. Furthermore, given the overlap between scRNA-seq and bulk RNA-seq analysis workflows, ASAP should conceptually be broadly applicable to any RNA-seq dataset. As a validation, we demonstrate how we can use ASAP to simply reproduce the results from a single-cell study of 91 mouse cells involving five distinct cell types. The tool is freely available at asap.epfl.ch and R/Python scripts are available at github.com/DeplanckeLab/ASAP. bart.deplancke@epfl.ch. Supplementary data are available at Bioinformatics online. © The Author(s) 2017. Published by Oxford University Press.

A large-scale superhydrophobic surface-enhanced Raman scattering (SERS) platform fabricated via capillary force lithography and assembly of Ag nanocubes for ultratrace molecular sensing.

Science.gov (United States)

Tan, Joel Ming Rui; Ruan, Justina Jiexin; Lee, Hiang Kwee; Phang, In Yee; Ling, Xing Yi

2014-12-28

An analytical platform with an ultratrace detection limit in the atto-molar (aM) concentration range is vital for forensic, industrial and environmental sectors that handle scarce/highly toxic samples. Superhydrophobic surface-enhanced Raman scattering (SERS) platforms serve as ideal platforms to enhance detection sensitivity by reducing the random spreading of aqueous solution. However, the fabrication of superhydrophobic SERS platforms is generally limited due to the use of sophisticated and expensive protocols and/or suffers structural and signal inconsistency. Herein, we demonstrate a high-throughput fabrication of a stable and uniform superhydrophobic SERS platform for ultratrace molecular sensing. Large-area box-like micropatterns of the polymeric surface are first fabricated using capillary force lithography (CFL). Subsequently, plasmonic properties are incorporated into the patterned surfaces by decorating with Ag nanocubes using the Langmuir-Schaefer technique. To create a stable superhydrophobic SERS platform, an additional 25 nm Ag film is coated over the Ag nanocube-decorated patterned template followed by chemical functionalization with perfluorodecanethiol. Our resulting superhydrophobic SERS platform demonstrates excellent water-repellency with a static contact angle of 165° ± 9° and a consequent analyte concentration factor of 59-fold, as compared to its hydrophilic counterpart. By combining the analyte concentration effect of superhydrophobic surfaces with the intense electromagnetic "hot spots" of Ag nanocubes, our superhydrophobic SERS platform achieves an ultra-low detection limit of 10(-17) M (10 aM) for rhodamine 6G using just 4 μL of analyte solutions, corresponding to an analytical SERS enhancement factor of 10(13). Our fabrication protocol demonstrates a simple, cost- and time-effective approach for the large-scale fabrication of a superhydrophobic SERS platform for ultratrace molecular detection.

The Transcriptome Analysis and Comparison Explorer--T-ACE: a platform-independent, graphical tool to process large RNAseq datasets of non-model organisms.

Science.gov (United States)

Philipp, E E R; Kraemer, L; Mountfort, D; Schilhabel, M; Schreiber, S; Rosenstiel, P

2012-03-15

Next generation sequencing (NGS) technologies allow a rapid and cost-effective compilation of large RNA sequence datasets in model and non-model organisms. However, the storage and analysis of transcriptome information from different NGS platforms is still a significant bottleneck, leading to a delay in data dissemination and subsequent biological understanding. Especially database interfaces with transcriptome analysis modules going beyond mere read counts are missing. Here, we present the Transcriptome Analysis and Comparison Explorer (T-ACE), a tool designed for the organization and analysis of large sequence datasets, and especially suited for transcriptome projects of non-model organisms with little or no a priori sequence information. T-ACE offers a TCL-based interface, which accesses a PostgreSQL database via a php-script. Within T-ACE, information belonging to single sequences or contigs, such as annotation or read coverage, is linked to the respective sequence and immediately accessible. Sequences and assigned information can be searched via keyword- or BLAST-search. Additionally, T-ACE provides within and between transcriptome analysis modules on the level of expression, GO terms, KEGG pathways and protein domains. Results are visualized and can be easily exported for external analysis. We developed T-ACE for laboratory environments, which have only a limited amount of bioinformatics support, and for collaborative projects in which different partners work on the same dataset from different locations or platforms (Windows/Linux/MacOS). For laboratories with some experience in bioinformatics and programming, the low complexity of the database structure and open-source code provides a framework that can be customized according to the different needs of the user and transcriptome project.

Low-Temperature and Rapid Growth of Large Single-Crystalline Graphene with Ethane.

Science.gov (United States)

Sun, Xiao; Lin, Li; Sun, Luzhao; Zhang, Jincan; Rui, Dingran; Li, Jiayu; Wang, Mingzhan; Tan, Congwei; Kang, Ning; Wei, Di; Xu, H Q; Peng, Hailin; Liu, Zhongfan

2018-01-01

Future applications of graphene rely highly on the production of large-area high-quality graphene, especially large single-crystalline graphene, due to the reduction of defects caused by grain boundaries. However, current large single-crystalline graphene growing methodologies are suffering from low growth rate and as a result, industrial graphene production is always confronted by high energy consumption, which is primarily caused by high growth temperature and long growth time. Herein, a new growth condition achieved via ethane being the carbon feedstock to achieve low-temperature yet rapid growth of large single-crystalline graphene is reported. Ethane condition gives a growth rate about four times faster than methane, achieving about 420 µm min -1 for the growth of sub-centimeter graphene single crystals at temperature about 1000 °C. In addition, the temperature threshold to obtain graphene using ethane can be reduced to 750 °C, lower than the general growth temperature threshold (about 1000 °C) with methane on copper foil. Meanwhile ethane always keeps higher graphene growth rate than methane under the same growth temperature. This study demonstrates that ethane is indeed a potential carbon source for efficient growth of large single-crystalline graphene, thus paves the way for graphene in high-end electronical and optoelectronical applications. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Cloud Based Earth Observation Data Exploitation Platforms

Science.gov (United States)

Romeo, A.; Pinto, S.; Loekken, S.; Marin, A.

2017-12-01

In the last few years data produced daily by several private and public Earth Observation (EO) satellites reached the order of tens of Terabytes, representing for scientists and commercial application developers both a big opportunity for their exploitation and a challenge for their management. New IT technologies, such as Big Data and cloud computing, enable the creation of web-accessible data exploitation platforms, which offer to scientists and application developers the means to access and use EO data in a quick and cost effective way. RHEA Group is particularly active in this sector, supporting the European Space Agency (ESA) in the Exploitation Platforms (EP) initiative, developing technology to build multi cloud platforms for the processing and analysis of Earth Observation data, and collaborating with larger European initiatives such as the European Plate Observing System (EPOS) and the European Open Science Cloud (EOSC). An EP is a virtual workspace, providing a user community with access to (i) large volume of data, (ii) algorithm development and integration environment, (iii) processing software and services (e.g. toolboxes, visualization routines), (iv) computing resources, (v) collaboration tools (e.g. forums, wiki, etc.). When an EP is dedicated to a specific Theme, it becomes a Thematic Exploitation Platform (TEP). Currently, ESA has seven TEPs in a pre-operational phase dedicated to geo-hazards monitoring and prevention, costal zones, forestry areas, hydrology, polar regions, urban areas and food security. On the technology development side, solutions like the multi cloud EO data processing platform provides the technology to integrate ICT resources and EO data from different vendors in a single platform. In particular it offers (i) Multi-cloud data discovery, (ii) Multi-cloud data management and access and (iii) Multi-cloud application deployment. This platform has been demonstrated with the EGI Federated Cloud, Innovation Platform Testbed Poland

Single Electron Transistor Platform for Microgravity Proteomics, Phase II

Data.gov (United States)

National Aeronautics and Space Administration — This Phase II program builds from the successful Phase I efforts to demonstrate that Quantum Logic Devices' nanoelectronic platform for biological detection could...

Single software platform used for high speed data transfer implementation in a 65k pixel camera working in single photon counting mode

International Nuclear Information System (INIS)

Maj, P.; Kasiński, K.; Gryboś, P.; Szczygieł, R.; Kozioł, A.

2015-01-01

Integrated circuits designed for specific applications generally use non-standard communication methods. Hybrid pixel detector readout electronics produces a huge amount of data as a result of number of frames per seconds. The data needs to be transmitted to a higher level system without limiting the ASIC's capabilities. Nowadays, the Camera Link interface is still one of the fastest communication methods, allowing transmission speeds up to 800 MB/s. In order to communicate between a higher level system and the ASIC with a dedicated protocol, an FPGA with dedicated code is required. The configuration data is received from the PC and written to the ASIC. At the same time, the same FPGA should be able to transmit the data from the ASIC to the PC at the very high speed. The camera should be an embedded system enabling autonomous operation and self-monitoring. In the presented solution, at least three different hardware platforms are used—FPGA, microprocessor with real-time operating system and the PC with end-user software. We present the use of a single software platform for high speed data transfer from 65k pixel camera to the personal computer

Single software platform used for high speed data transfer implementation in a 65k pixel camera working in single photon counting mode

Science.gov (United States)

Maj, P.; Kasiński, K.; Gryboś, P.; Szczygieł, R.; Kozioł, A.

2015-12-01

Integrated circuits designed for specific applications generally use non-standard communication methods. Hybrid pixel detector readout electronics produces a huge amount of data as a result of number of frames per seconds. The data needs to be transmitted to a higher level system without limiting the ASIC's capabilities. Nowadays, the Camera Link interface is still one of the fastest communication methods, allowing transmission speeds up to 800 MB/s. In order to communicate between a higher level system and the ASIC with a dedicated protocol, an FPGA with dedicated code is required. The configuration data is received from the PC and written to the ASIC. At the same time, the same FPGA should be able to transmit the data from the ASIC to the PC at the very high speed. The camera should be an embedded system enabling autonomous operation and self-monitoring. In the presented solution, at least three different hardware platforms are used—FPGA, microprocessor with real-time operating system and the PC with end-user software. We present the use of a single software platform for high speed data transfer from 65k pixel camera to the personal computer.

A single-cell analysis platform for electrochemiluminescent detection of platelets adhesion to endothelial cells based on Au@DL-ZnCQDs nanoprobes.

Science.gov (United States)

Long, Dongping; Shang, Yunfei; Qiu, Youyi; Zhou, Bin; Yang, Peihui

2018-04-15

A novel single-cell analysis platform (SCA) was developed for the investigation of platelets adhesion to single human umbilical vein endothelial cell (HUVEC) via using the adhesion molecule (E-selectin) on the damaged HUVEC as the marker site, and integrating electrochemiluminescence (ECL) with the ultrasensitive Au@DL-ZnCQDs nanoprobes. The Au@DL-ZnCQDs nanocomposite, a kind of double layer zinc-coadsorbed carbon quantum dot (ZnCQDs) core-shell nanoprobe, was firstly constructed by using gold nanoparticles (AuNPs) as the core to load with ZnCQDs and then the citrate-modified silver nanoparticles (AgNPs) as the bridge to link AuNPs-ZnCQDs with ZnCQDs to form the core-shell with double layer ZnCQDs (DL-ZnCQDs) nanoprobe, revealed a 10-fold signal amplification. The H 2 O 2 -induced oxidative damage HUVECs were utilized as the cellular model on which anti-E-selectin functionalized nanoprobes specially recognized E-selectin, the SCA showed that the ECL signals decreased with platelets adhesion to single HUVEC. The proposed SCA could effectively and dynamically monitor the adhesion between single HUVEC and platelets in the absence and presence of collagen activation, moreover, be able to quantitatively detect the number of platelets adhesion to single HUVEC, and show a good analytical performance with linear range from 1 to 15 platelets. In contrast, the HUVEC was down-regulated the expression of adhesion molecules by treating with quercetin inhibitor, and the SCA also exhibited the feasibility for analysis of platelets adhesion to single HUVEC. Therefore, the single-cell analysis platform provided a novel and promising protocol for analysis of the single intercellular adhesion, and it will be beneficial to elucidate the pathogenesis of cardiovascular diseases. Copyright © 2017 Elsevier B.V. All rights reserved.

Identification of platform levels

DEFF Research Database (Denmark)

Mortensen, Niels Henrik

2005-01-01

reduction, ability to launch a wider product portfolio without increasing resources and reduction of complexity within the whole company. To support the multiple product development process, platform based product development has in many companies such as Philips, VW, Ford etc. proven to be a very effective...... product development in one step and therefore the objective of this paper is to identify levels of platform based product development. The structure of this paper is as follows. First the applied terminology for platforms will be briefly explained and then characteristics between single and multi product...... development will be examined. Based on the identification of the above characteristics five platform levels are described. The research presented in this paper is a result of MSc, Ph.D projects at the Technical University of Denmark and consultancy projects within the organisation of Institute of Product...

Large-scale quantum photonic circuits in silicon

Directory of Open Access Journals (Sweden)

Harris Nicholas C.

2016-08-01

Full Text Available Quantum information science offers inherently more powerful methods for communication, computation, and precision measurement that take advantage of quantum superposition and entanglement. In recent years, theoretical and experimental advances in quantum computing and simulation with photons have spurred great interest in developing large photonic entangled states that challenge today’s classical computers. As experiments have increased in complexity, there has been an increasing need to transition bulk optics experiments to integrated photonics platforms to control more spatial modes with higher fidelity and phase stability. The silicon-on-insulator (SOI nanophotonics platform offers new possibilities for quantum optics, including the integration of bright, nonclassical light sources, based on the large third-order nonlinearity (χ(3 of silicon, alongside quantum state manipulation circuits with thousands of optical elements, all on a single phase-stable chip. How large do these photonic systems need to be? Recent theoretical work on Boson Sampling suggests that even the problem of sampling from e30 identical photons, having passed through an interferometer of hundreds of modes, becomes challenging for classical computers. While experiments of this size are still challenging, the SOI platform has the required component density to enable low-loss and programmable interferometers for manipulating hundreds of spatial modes.

Staghorn: An Automated Large-Scale Distributed System Analysis Platform

Energy Technology Data Exchange (ETDEWEB)

Gabert, Kasimir [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Burns, Ian [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Elliott, Steven [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Kallaher, Jenna [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Vail, Adam [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

2016-09-01

Conducting experiments on large-scale distributed computing systems is becoming significantly easier with the assistance of emulation. Researchers can now create a model of a distributed computing environment and then generate a virtual, laboratory copy of the entire system composed of potentially thousands of virtual machines, switches, and software. The use of real software, running at clock rate in full virtual machines, allows experiments to produce meaningful results without necessitating a full understanding of all model components. However, the ability to inspect and modify elements within these models is bound by the limitation that such modifications must compete with the model, either running in or alongside it. This inhibits entire classes of analyses from being conducted upon these models. We developed a mechanism to snapshot an entire emulation-based model as it is running. This allows us to \\freeze time" and subsequently fork execution, replay execution, modify arbitrary parts of the model, or deeply explore the model. This snapshot includes capturing packets in transit and other input/output state along with the running virtual machines. We were able to build this system in Linux using Open vSwitch and Kernel Virtual Machines on top of Sandia's emulation platform Firewheel. This primitive opens the door to numerous subsequent analyses on models, including state space exploration, debugging distributed systems, performance optimizations, improved training environments, and improved experiment repeatability.

Role of optometry school in single day large scale school vision testing

Science.gov (United States)

Anuradha, N; Ramani, Krishnakumar

2015-01-01

Background: School vision testing aims at identification and management of refractive errors. Large-scale school vision testing using conventional methods is time-consuming and demands a lot of chair time from the eye care professionals. A new strategy involving a school of optometry in single day large scale school vision testing is discussed. Aim: The aim was to describe a new approach of performing vision testing of school children on a large scale in a single day. Materials and Methods: A single day vision testing strategy was implemented wherein 123 members (20 teams comprising optometry students and headed by optometrists) conducted vision testing for children in 51 schools. School vision testing included basic vision screening, refraction, frame measurements, frame choice and referrals for other ocular problems. Results: A total of 12448 children were screened, among whom 420 (3.37%) were identified to have refractive errors. 28 (1.26%) children belonged to the primary, 163 to middle (9.80%), 129 (4.67%) to secondary and 100 (1.73%) to the higher secondary levels of education respectively. 265 (2.12%) children were referred for further evaluation. Conclusion: Single day large scale school vision testing can be adopted by schools of optometry to reach a higher number of children within a short span. PMID:25709271

Ideas for future large single dish radio telescopes

Science.gov (United States)

Kärcher, Hans J.; Baars, Jacob W. M.

2014-07-01

The existing large single dish radio telescopes of the 100m class (Effelsberg, Green Bank) were built in the 1970s and 1990s. With some active optics they work now down to 3 millimeter wavelength where the atmospheric quality of the site is also a limiting factor. Other smaller single dish telescopes (50m LMT Mexico, 30m IRAM Spain) are located higher and reach sub-millimeter quality, and the much smaller 12m antennas of the ALMA array reach at a very high site the Terahertz region. They use advanced technologies as carbon fiber structures and flexible body control. We review natural limits to telescope design and use the examples of a number of telescopes for an overview of the available state-of-the-art in design, engineering and technologies. Without considering the scientific justification we then offer suggestions to realize ultimate performance of huge single dish telescopes (up to 160m). We provide an outlook on design options, technological frontiers and cost estimates.

High quality single crystal Ge nano-membranes for opto-electronic integrated circuitry

Energy Technology Data Exchange (ETDEWEB)

Shah, V. A., E-mail: vishal.shah@warwick.ac.uk; Gammon, P. M. [Department of Engineering, The University of Warwick, Coventry CV4 7AL (United Kingdom); Department of Physics, The University of Warwick, Coventry CV4 7AL (United Kingdom); Rhead, S. D.; Halpin, J. E.; Trushkevych, O.; Wilson, N. R.; Myronov, M.; Edwards, R. S.; Patchett, D. H.; Allred, P. S.; Prest, M. J.; Whall, T. E.; Parker, E. H. C.; Leadley, D. R. [Department of Physics, The University of Warwick, Coventry CV4 7AL (United Kingdom); Chávez-Ángel, E. [ICN2-Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, 08193 Bellaterra (Barcelona) (Spain); Department of Physics, UAB, 08193 Bellaterra (Barcelona) (Spain); Shchepetov, A.; Prunnila, M. [VTT Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT, Espoo (Finland); Kachkanov, V.; Dolbnya, I. P. [Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE (United Kingdom); Reparaz, J. S. [ICN2-Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, 08193 Bellaterra (Barcelona) (Spain); and others

2014-04-14

A thin, flat, and single crystal germanium membrane would be an ideal platform on which to mount sensors or integrate photonic and electronic devices, using standard silicon processing technology. We present a fabrication technique compatible with integrated-circuit wafer scale processing to produce membranes of thickness between 60 nm and 800 nm, with large areas of up to 3.5 mm{sup 2}. We show how the optical properties change with thickness, including appearance of Fabry-Pérot type interference in thin membranes. The membranes have low Q-factors, which allow the platforms to counteract distortion during agitation and movement. Finally, we report on the physical characteristics showing sub-nm roughness and a homogenous strain profile throughout the freestanding layer, making the single crystal Ge membrane an excellent platform for further epitaxial growth or deposition of materials.

High quality single crystal Ge nano-membranes for opto-electronic integrated circuitry

International Nuclear Information System (INIS)

Shah, V. A.; Gammon, P. M.; Rhead, S. D.; Halpin, J. E.; Trushkevych, O.; Wilson, N. R.; Myronov, M.; Edwards, R. S.; Patchett, D. H.; Allred, P. S.; Prest, M. J.; Whall, T. E.; Parker, E. H. C.; Leadley, D. R.; Chávez-Ángel, E.; Shchepetov, A.; Prunnila, M.; Kachkanov, V.; Dolbnya, I. P.; Reparaz, J. S.

2014-01-01

A thin, flat, and single crystal germanium membrane would be an ideal platform on which to mount sensors or integrate photonic and electronic devices, using standard silicon processing technology. We present a fabrication technique compatible with integrated-circuit wafer scale processing to produce membranes of thickness between 60 nm and 800 nm, with large areas of up to 3.5 mm 2 . We show how the optical properties change with thickness, including appearance of Fabry-Pérot type interference in thin membranes. The membranes have low Q-factors, which allow the platforms to counteract distortion during agitation and movement. Finally, we report on the physical characteristics showing sub-nm roughness and a homogenous strain profile throughout the freestanding layer, making the single crystal Ge membrane an excellent platform for further epitaxial growth or deposition of materials

RPA-coated single-stranded DNA as a platform for post-translational modifications in the DNA damage response.

Science.gov (United States)

Maréchal, Alexandre; Zou, Lee

2015-01-01

The Replication Protein A (RPA) complex is an essential regulator of eukaryotic DNA metabolism. RPA avidly binds to single-stranded DNA (ssDNA) through multiple oligonucleotide/oligosaccharide-binding folds and coordinates the recruitment and exchange of genome maintenance factors to regulate DNA replication, recombination and repair. The RPA-ssDNA platform also constitutes a key physiological signal which activates the master ATR kinase to protect and repair stalled or collapsed replication forks during replication stress. In recent years, the RPA complex has emerged as a key target and an important regulator of post-translational modifications in response to DNA damage, which is critical for its genome guardian functions. Phosphorylation and SUMOylation of the RPA complex, and more recently RPA-regulated ubiquitination, have all been shown to control specific aspects of DNA damage signaling and repair by modulating the interactions between RPA and its partners. Here, we review our current understanding of the critical functions of the RPA-ssDNA platform in the maintenance of genome stability and its regulation through an elaborate network of covalent modifications.

Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization

OpenAIRE

Fan, Beiyuan; Li, Xiufeng; Chen, Deyong; Peng, Hongshang; Wang, Junbo; Chen, Jian

2016-01-01

This article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry; (3) large-array micro wells (microengraving); and (4) large-array micro chambers (barcode microchips). We examine the advantages and limitations of each technique and discuss future research oppor...

A platform for exploding wires in different media

Science.gov (United States)

Han, Ruoyu; Wu, Jiawei; Qiu, Aici; Zhou, Haibin; Wang, Yanan; Yan, Jiaqi; Ding, Weidong

2017-10-01

A platform SWE-2 used for single wire explosion experiments has been designed, established, and commissioned. This paper describes the design and initial experiments of SWE-2. In summary, two pulsed current sources based on pulse capacitors and spark gaps are adopted to drive sub-microsecond and microsecond time scale wire explosions in a gaseous/liquid medium, respectively. In the initial experiments, a single copper wire was exploded in air, helium, and argon with a 0.1-0.3 MPa ambient pressure as well as tap water with a 283-323 K temperature, 184-11 000 μ S/cm conductivity, or 0.1-0.9 MPa hydrostatic pressure. In addition, the diagnostic system is introduced in detail. Energy deposition, optical emission, and shock wave characteristics are briefly discussed based on experimental results. The platform was demonstrated to operate successfully with a single wire load. These results provide the potential for further applications of this platform, such as plasma-matter interactions, shock wave effects, and reservoir simulations.

Large rotating magnetocaloric effect in ErAlO3 single crystal

Directory of Open Access Journals (Sweden)

X. Q. Zhang

2017-05-01

Full Text Available Magnetic and magnetocaloric properties of ErAlO3 single crystal were investigated. Magnetization of ErAlO3 shows obvious anisotropy when magnetic field is applied along the a, b and c axes, which leads to large anisotropic magnetic entropy change. In particular, large rotating field entropy change from the b to c axis within the bc plane is obtained and reaches 9.7 J/kg K at 14 K in a field of 5 T. This suggests the possibility of using ErAlO3 single crystal for magnetic refrigerators by rotating its magnetization vector rather than moving it in and out of the magnet.

Software Development Process Improvement in Datacom Platform

OpenAIRE

Trabelsi, Walid

2008-01-01

Masteroppgave i Informasjons- og Kommunikasjonsteknologi 2008, Universitetet i Agder, Grimstad Ericsson Mobile Platform (EMP) is responsible of the development of a software platform and also to some extend responsible for related hardware parts. EMP is developing the data communication parts of the platform which is used by EMP customers. The platform development is done in large development programs and each program span over a quite a long time period. However, as we see eve...

Large-scale membrane transfer process: its application to single-crystal-silicon continuous membrane deformable mirror

International Nuclear Information System (INIS)

Wu, Tong; Sasaki, Takashi; Hane, Kazuhiro; Akiyama, Masayuki

2013-01-01

This paper describes a large-scale membrane transfer process developed for the construction of large-scale membrane devices via the transfer of continuous single-crystal-silicon membranes from one substrate to another. This technique is applied for fabricating a large stroke deformable mirror. A bimorph spring array is used to generate a large air gap between the mirror membrane and the electrode. A 1.9 mm × 1.9 mm × 2 µm single-crystal-silicon membrane is successfully transferred to the electrode substrate by Au–Si eutectic bonding and the subsequent all-dry release process. This process provides an effective approach for transferring a free-standing large continuous single-crystal-silicon to a flexible suspension spring array with a large air gap. (paper)

Microfluidic platform for efficient Nanodisc assembly, membrane protein incorporation, and purification.

Science.gov (United States)

Wade, James H; Jones, Joshua D; Lenov, Ivan L; Riordan, Colleen M; Sligar, Stephen G; Bailey, Ryan C

2017-08-22

The characterization of integral membrane proteins presents numerous analytical challenges on account of their poor activity under non-native conditions, limited solubility in aqueous solutions, and low expression in most cell culture systems. Nanodiscs are synthetic model membrane constructs that offer many advantages for studying membrane protein function by offering a native-like phospholipid bilayer environment. The successful incorporation of membrane proteins within Nanodiscs requires experimental optimization of conditions. Standard protocols for Nanodisc formation can require large amounts of time and input material, limiting the facile screening of formation conditions. Capitalizing on the miniaturization and efficient mass transport inherent to microfluidics, we have developed a microfluidic platform for efficient Nanodisc assembly and purification, and demonstrated the ability to incorporate functional membrane proteins into the resulting Nanodiscs. In addition to working with reduced sample volumes, this platform simplifies membrane protein incorporation from a multi-stage protocol requiring several hours or days into a single platform that outputs purified Nanodiscs in less than one hour. To demonstrate the utility of this platform, we incorporated Cytochrome P450 into Nanodiscs of variable size and lipid composition, and present spectroscopic evidence for the functional active site of the membrane protein. This platform is a promising new tool for membrane protein biology and biochemistry that enables tremendous versatility for optimizing the incorporation of membrane proteins using microfluidic gradients to screen across diverse formation conditions.

Stratospheric Platforms for Monitoring Purposes

International Nuclear Information System (INIS)

Konigorski, D.; Gratzel, U.; Obersteiner, M.; Schneidereit, M.

2010-01-01

Stratospheric platforms are emerging systems based on challenging technology. Goal is to create a platform, payload, and mission design which is able to complement satellite services on a local scale. Applications are close to traditional satellite business in telecommunication, navigation, science, and earth observation and include for example mobile telecommunications, navigation augmentation, atmospheric research, or border control. Stratospheric platforms could potentially support monitoring activities related to safeguards, e.g. by imagery of surfaces, operational conditions of nuclear facilities, and search for undeclared nuclear activities. Stratospheric platforms are intended to be flown in an altitude band between 16 and 30 km, above 16-20 km to take advantage of usually lower winds facilitating station keeping, below 30 km to limit the challenges to achieve a reasonable payload at acceptable platform sizes. Stratospheric platforms could substitute satellites which are expensive and lack upgrade capabilities for new equipment. Furthermore they have practically an unlimited time over an area of interest. It is intended to keep the platforms operational and maintenance free on a 24/7 basis with an average deployment time of 3 years. Geostationary satellites lack resolution. Potential customers like Armed Forces, National Agencies and commercial customers have indicated interest in the use of stratospheric platforms. Governmental entities are looking for cheaper alternatives to communications and surveillance satellites and stratospheric platforms could offer the following potential advantages: Lower operational cost than satellite or UAV (Unmanned Aerial Vehicles) constellation (fleet required); Faster deployment than satellite constellation; Repositioning capability and ability to loiter as required; Persistent long-term real-time services over a fairly large regional spot; Surge capability: Able to extend capability (either monitoring or communications

Cretaceous tropical carbonate platform changes used as paleoclimatic and paleoceanic indicators: the three lower Cretaceous platform crises

Science.gov (United States)

Arnaud-Vanneau, A.; Vrielynck, B.

2009-04-01

Carbonate platform sediments are of biogenic origin. More commonly the bioclasts are fragments of shells and skeletons. The bioclastic composition of a limestone may reflect the nature of biota inhabiting the area and a carbonate platform can be estimated as a living factory, which reflects the prevailing ecological factors. The rate of carbonate production is highest in the tropics, in oligotrophic environments, and in the photic zone. The rate of carbonate production varies greatly with temperature and nutrient input. Three types of biotic carbonate platform can be distinguished. The highest carbonate production is linked to oligotrophic carbonate platform characterized by the presence of assemblages with hermatypic corals. This type of platform is developed in shallow marine environment, nutrient poor water and warm tropical sea. A less efficient production of carbonate platform is related to mesotrophic environments in cooler and/or deeper water and associated to nutrient flux with, sometime, detrital input. The biota includes red algae, solitary coral and branching ahermatypic corals, common bryozoans, crinoids and echinoids. The less productive carbonate platform is the eutrophic muddy platform where the mud is due to the intense bacterial activity, probably related to strong nutrient flux. All changes of type of carbonate platform can be related to climatic and oceanic changes. Three platform crises occurred during lower Cretaceous time. They are followed by important turnover of microfauna (large benthic foraminifers) and microflora (marine algae). They start with the demise of the previous oligotrophic platform, they continue with oceanic perturbations, expression of which was the widespread deposition of organic-rich sediments, well expressed during Late Aptian/Albian and Cenomanian Turonian boundary and the replacement of previous oligotrophic platforms by mesotrophic to eutrophic platforms. The first crisis occurred during Valanginian and Hauterivian

Development of integrated platform for computational material design

Energy Technology Data Exchange (ETDEWEB)

Kiyoshi, Matsubara; Kumi, Itai; Nobutaka, Nishikawa; Akifumi, Kato [Center for Computational Science and Engineering, Fuji Research Institute Corporation (Japan); Hideaki, Koike [Advance Soft Corporation (Japan)

2003-07-01

The goal of our project is to design and develop a problem-solving environment (PSE) that will help computational scientists and engineers develop large complicated application software and simulate complex phenomena by using networking and parallel computing. The integrated platform, which is designed for PSE in the Japanese national project of Frontier Simulation Software for Industrial Science, is defined by supporting the entire range of problem solving activity from program formulation and data setup to numerical simulation, data management, and visualization. A special feature of our integrated platform is based on a new architecture called TASK FLOW. It integrates the computational resources such as hardware and software on the network and supports complex and large-scale simulation. This concept is applied to computational material design and the project 'comprehensive research for modeling, analysis, control, and design of large-scale complex system considering properties of human being'. Moreover this system will provide the best solution for developing large and complicated software and simulating complex and large-scaled phenomena in computational science and engineering. A prototype has already been developed and the validation and verification of an integrated platform will be scheduled by using the prototype in 2003. In the validation and verification, fluid-structure coupling analysis system for designing an industrial machine will be developed on the integrated platform. As other examples of validation and verification, integrated platform for quantum chemistry and bio-mechanical system are planned.

Development of integrated platform for computational material design

International Nuclear Information System (INIS)

Kiyoshi, Matsubara; Kumi, Itai; Nobutaka, Nishikawa; Akifumi, Kato; Hideaki, Koike

2003-01-01

The goal of our project is to design and develop a problem-solving environment (PSE) that will help computational scientists and engineers develop large complicated application software and simulate complex phenomena by using networking and parallel computing. The integrated platform, which is designed for PSE in the Japanese national project of Frontier Simulation Software for Industrial Science, is defined by supporting the entire range of problem solving activity from program formulation and data setup to numerical simulation, data management, and visualization. A special feature of our integrated platform is based on a new architecture called TASK FLOW. It integrates the computational resources such as hardware and software on the network and supports complex and large-scale simulation. This concept is applied to computational material design and the project 'comprehensive research for modeling, analysis, control, and design of large-scale complex system considering properties of human being'. Moreover this system will provide the best solution for developing large and complicated software and simulating complex and large-scaled phenomena in computational science and engineering. A prototype has already been developed and the validation and verification of an integrated platform will be scheduled by using the prototype in 2003. In the validation and verification, fluid-structure coupling analysis system for designing an industrial machine will be developed on the integrated platform. As other examples of validation and verification, integrated platform for quantum chemistry and bio-mechanical system are planned

Copper-free click-chemistry platform to functionalize cisplatin prodrugs.

Science.gov (United States)

Pathak, Rakesh K; McNitt, Christopher D; Popik, Vladimir V; Dhar, Shanta

2014-06-02

The ability to rationally design and construct a platform technology to develop new platinum(IV) [Pt(IV)] prodrugs with functionalities for installation of targeting moieties, delivery systems, fluorescent reporters from a single precursor with the ability to release biologically active cisplatin by using well-defined chemistry is critical for discovering new platinum-based therapeutics. With limited numbers of possibilities considering the sensitivity of Pt(IV) centers, we used a strain-promoted azide-alkyne cycloaddition approach to provide a platform, in which new functionalities can easily be installed on cisplatin prodrugs from a single Pt(IV) precursor. The ability of this platform to be incorporated in nanodelivery vehicle and conjugation to fluorescent reporters were also investigated. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

An Integrated System for Disabled People Developed with the Agent Platform PANGEA

Directory of Open Access Journals (Sweden)

Carolina ZATO

2013-11-01

Full Text Available New trends in multi-agent systems call for self-adaptation and high dynamics, hence the new model of open MAS or virtual organization of agents. However, as existing agent platforms are not yet equipped to support this behavior, it is necessary to create new systems and mechanisms to facilitate the development of these new architectures. This article presents PANGEA, an agent platform to develop open multi-agent systems, specifically those including organizational aspects such as virtual agent organizations. The platform allows the integral management of organizations and offers tools to the end user. Additionally, it includes a communication protocol based on the IRC standard, which facilitates implementation and remains robust even with a large number of connections. The introduction of a CommunicationAgent and a Sniffer make it possible to offer Web Services for the distributed control of interaction. In order to test PANGEA, an integral system was developed to help the disabled, gathering a set of easily deployable and integrated services under a single architecture.

The Definitive Guide to NetBeans™ Platform 7

CERN Document Server

Bock, Heiko

2011-01-01

The NetBeans Platform is the world's only modular Swing application framework, used by very large organizations in mission-critical scenarios, such as at Boeing and Northrop Grumman, as well as in the financial sector and in the oil/gas industry. For these large customers in enterprises who are increasingly interested in Maven and OSGi, the book will have particular relevance. The Definitive Guide to NetBeans Platform 7 is a thorough and authoritative introduction to the open-source NetBeans Platform, covering all its major APIs in detail, with relevant code examples used throughout. * Provide

Fabrication of single optical centres in diamond-a review

International Nuclear Information System (INIS)

Orwa, J.O.; Greentree, A.D.; Aharonovich, I.; Alves, A.D.C.; Van Donkelaar, J.; Stacey, A.; Prawer, S.

2010-01-01

Colour centres in diamond are rapidly becoming one of the leading platforms for solid-state quantum information processing applications. This is due in large part to the remarkable properties of the nitrogen-vacancy colour centre. From initial demonstrations of room-temperature single photon generation and spin single spin readout and quantum control, diamond nanocrystals are also finding application in magnetometry and biosensing. This review discusses the state of the art in the creation of isolated and small ensembles of optically active diamond defect centres, including nitrogen and nickel-related centres.

Large-scale single-chirality separation of single-wall carbon nanotubes by simple gel chromatography

Science.gov (United States)

Liu, Huaping; Nishide, Daisuke; Tanaka, Takeshi; Kataura, Hiromichi

2011-01-01

Monostructured single-wall carbon nanotubes (SWCNTs) are important in both scientific research and electronic and biomedical applications; however, the bulk separation of SWCNTs into populations of single-chirality nanotubes remains challenging. Here we report a simple and effective method for the large-scale chirality separation of SWCNTs using a single-surfactant multicolumn gel chromatography method utilizing one surfactant and a series of vertically connected gel columns. This method is based on the structure-dependent interaction strength of SWCNTs with an allyl dextran-based gel. Overloading an SWCNT dispersion on the top column results in the adsorption sites of the column becoming fully occupied by the nanotubes that exhibit the strongest interaction with the gel. The unbound nanotubes flow through to the next column, and the nanotubes with the second strongest interaction with the gel are adsorbed in this stage. In this manner, 13 different (n, m) species were separated. Metallic SWCNTs were finally collected as unbound nanotubes because they exhibited the lowest interaction with the gel. PMID:21556063

On the notion of abstract platform in MDA development

NARCIS (Netherlands)

Andrade Almeida, João; Dijkman, R.M.; van Sinderen, Marten J.; Ferreira Pires, Luis

2004-01-01

Although platform-independence is a central property in MDA models, the study of platform-independence has been largely overlooked in MDA. As a consequence, there is a lack of guidelines to select abstraction criteria and modelling concepts for platform-independent design. In addition, there is

Design and analysis of power supply and information transfer to three-axis gyroscope stabilizer platform

Directory of Open Access Journals (Sweden)

Belyanin Lev

2016-01-01

Full Text Available The paper focuses on problems of the engineering implementation of 3-axis gyrostabilizer of the camera for orthophotography performed from light and ultralight aircrafts. The functional diagram of gyrostabilizer is characterized by a lighter ball-bearing having a large inner diameter used for the platform suspension on its internal (vertical axis. The problem is to transfer the supply voltage to the platform and the electric signals to and from the platform. Design solutions concerning the power supply and information transfer are supported by the experimental techniques suggested in this paper, namely: the use of the rolling contact; three-phase gyro motor power supply replaced by that from the single-phase network; signal transmission from the precession angle sensor using the air transformer; current and voltage frequency division; DC mode selection for the torquer. The results obtained prove the efficiency of the suggested design solutions.

An Investigation of Digital Payment Platform Designs

DEFF Research Database (Denmark)

Kazan, Erol; Damsgaard, Jan

2014-01-01

This paper focuses on the triumph march of mobile phones that currently are annexing music players, navigation devices, and cameras as separate physical objects. The next target is set on payment. Through synthesizing available literature, we construct a framework for studying digital payment...... platforms that combines platform, technology and business design aspects. The framework is applied to conduct a comparative case study of digital payment platforms. Four types of market actors are considered: banks, mobile network operators, merchants, and startups, which are incumbents and disrupters....... By hosting third-party services, payment instruments are evolving from single-purpose to multi-functional ones. Our research extends existing payment literature from the MSP perspective to emphasize certain digital payment platform components, which impact strategies and complementary products....

The universal modular platform

International Nuclear Information System (INIS)

North, R.B.

1995-01-01

A new and patented design for offshore wellhead platforms has been developed to meet a 'fast track' requirement for increased offshore production, from field locations not yet identified. The new design uses modular construction to allow for radical changes in the water depth of the final location and assembly line efficiency in fabrication. By utilizing high strength steels and structural support from the well conductors the new design accommodates all planned production requirements on a support structure significantly lighter and less expensive than the conventional design it replaces. Twenty two platforms based on the new design were ready for installation within 18 months of the project start. Installation of the new platforms began in 1992 for drilling support and 1993 for production support. The new design has become the Company standard for all future production platforms. Large saving and construction costs have been realized through its light weight, flexibility in both positioning and water depth, and its modular construction

Engaging Students in a Large-Enrollment Physics Class Using an Academically Focused Social Media Platform

Science.gov (United States)

Gavrin, Andy; Lindell, Rebecca

2017-03-01

There are many reasons for an instructor to consider using social media, particularly in a large introductory course. Improved communications can lessen the sense of isolation some students feel in large classes, and students may be more likely to respond to faculty announce-ments in a form that is familiar and comfortable. Furthermore, many students currently establish social media sites for their classes, without the knowledge or participation of their instructors. Such "shadow" sites can be useful, but they can also become distributors of misinformation, or venues for inappropriate or disruptive discussions. CourseNetworking (CN) is a social media platform designed for the academic environment. It combines many features common among learning management systems (LMS's) with an interface that looks and feels more like Facebook than a typical academic system. We have recently begun using CN as a means to engage students in an introductory calculus-based mechanics class, with enrollments of 150-200 students per semester. This article presents basic features of CN, and details our initial experiences and observations.

Cross-Platform Learning: On the Nature of Children's Learning from Multiple Media Platforms

Science.gov (United States)

Fisch, Shalom M.

2013-01-01

It is increasingly common for an educational media project to span several media platforms (e.g., TV, Web, hands-on materials), assuming that the benefits of learning from multiple media extend beyond those gained from one medium alone. Yet research typically has investigated learning from a single medium in isolation. This paper reviews several…

A study of rotor and platform design trade-offs for large-scale floating vertical axis wind turbines

Science.gov (United States)

Griffith, D. Todd; Paquette, Joshua; Barone, Matthew; Goupee, Andrew J.; Fowler, Matthew J.; Bull, Diana; Owens, Brian

2016-09-01

Vertical axis wind turbines are receiving significant attention for offshore siting. In general, offshore wind offers proximity to large populations centers, a vast & more consistent wind resource, and a scale-up opportunity, to name a few beneficial characteristics. On the other hand, offshore wind suffers from high levelized cost of energy (LCOE) and in particular high balance of system (BoS) costs owing to accessibility challenges and limited project experience. To address these challenges associated with offshore wind, Sandia National Laboratories is researching large-scale (MW class) offshore floating vertical axis wind turbines (VAWTs). The motivation for this work is that floating VAWTs are a potential transformative technology solution to reduce offshore wind LCOE in deep-water locations. This paper explores performance and cost trade-offs within the design space for floating VAWTs between the configurations for the rotor and platform.

Rejection of large HPV-16 expressing tumors in aged mice by a single immunization of VacciMax® encapsulated CTL/T helper peptides

Directory of Open Access Journals (Sweden)

MacDonald Lisa

2007-06-01

Full Text Available Abstract The incidence of cancer increases significantly in later life, yet few pre-clinical studies of cancer immunotherapy use mice of advanced age. A novel vaccine delivery platform (VacciMax®,VM is described that encapsulates antigens and adjuvants in multilamellar liposomes in a water-in-oil emulsion. The therapeutic potential of VM-based vaccines administered as a single dose was tested in HLA-A2 transgenic mice of advanced age (48–58 weeks old bearing large palpable TC1/A2 tumors. The VM-based vaccines contained one or more peptides having human CTL epitopes derived from HPV 16 E6 and E7. VM formulations contained a single peptide, a mixture of four peptides or the same four peptides linked together in a single long peptide. All VM formulations contained PADRE and CpG as adjuvants and ISA51 as the hydrophobic component of the water-in-oil emulsion. VM-formulated vaccines containing the four peptides as a mixture or linked together in one long peptide eradicated 19-day old established tumors within 21 days of immunization. Peptide-specific cytotoxic cellular responses were confirmed by ELISPOT and intracellular staining for IFN-γ producing CD8+ T cells. Mice rendered tumor-free by vaccination were re-challenged in the opposite flank with 10 million HLF-16 tumor cells, another HLA-A2/E6/E7 expressing tumor cell line. None of these mice developed tumors following the re-challenge. In summary, this report describes a VM-formulated therapeutic vaccine with the following unprecedented outcome: a eradication of large tumors (> 700 mm3 b in mice of advanced age c in less than three weeks post-immunization d following a single vaccination.

CERN single sign on solution

International Nuclear Information System (INIS)

Ormancey, E

2008-01-01

The need for Single Sign On has always been restricted by the absence of cross platform solutions: a single sign on working only on one platform or technology is nearly useless. The recent improvements in Web Services Federation (WS-Federation) standard enabling federation of identity, attribute, authentication and authorization information can now provide real extended Single Sign On solutions. Various solutions have been investigated at CERN and now, a Web SSO solution using some parts of WS-Federation technology is available. Using the Shibboleth Service Provider module for Apache hosted web sites and Microsoft ADFS as the identity provider linked to Active Directory user, users can now authenticate on any web application using a single authentication platform, providing identity, user information (building, phone...) as well as group membership enabling authorization possibilities. A typical scenario: a CERN user can now authenticate on a Linux/Apache website using Windows Integrated credentials, and his Active Directory group membership can be checked before allowing access to a specific web page

Single-photon manipulation in Nanophotonic Circuits

DEFF Research Database (Denmark)

Hansen, Sofie Lindskov

Quantum dots in photonic nanostructures has long been known to be a very powerful and versatile solid-state platform for conducting quantum optics experiments. The present PhD thesis describes experimental demonstrations of single-photon generation and subsequent manipulation all realized...... on a gallium arsenide platform. This platform offers near-unity coupling between embedded single-photon emitters and a photonic mode, as well as the ability to suppress decoherence mechanisms, making it highly suited for quantum information applications. In this thesis we show how a single-photon router can...... be realized on a chip with embedded quantum dots. This allows for on-chip generation and manipulation of single photons. The router consists of an on-chip interferometer where the phase difference between the arms of the interferometer is controlled electrically. The response time of the device...

The Epilepsy Phenome/Genome Project (EPGP) informatics platform.

Science.gov (United States)

Nesbitt, Gerry; McKenna, Kevin; Mays, Vickie; Carpenter, Alan; Miller, Kevin; Williams, Michael

2013-04-01

The Epilepsy Phenome/Genome Project (EPGP) is a large-scale, multi-institutional, collaborative network of 27 epilepsy centers throughout the U.S., Australia, and Argentina, with the objective of collecting detailed phenotypic and genetic data on a large number of epilepsy participants. The goals of EPGP are (1) to perform detailed phenotyping on 3750 participants with specific forms of non-acquired epilepsy and 1500 parents without epilepsy, (2) to obtain DNA samples on these individuals, and (3) to ultimately genotype the samples in order to discover novel genes that cause epilepsy. To carry out the project, a reliable and robust informatics platform was needed for standardized electronic data collection and storage, data quality review, and phenotypic analysis involving cases from multiple sites. EPGP developed its own suite of web-based informatics applications for participant tracking, electronic data collection (using electronic case report forms/surveys), data management, phenotypic data review and validation, specimen tracking, electroencephalograph and neuroimaging storage, and issue tracking. We implemented procedures to train and support end-users at each clinical site. Thus far, 3780 study participants have been enrolled and 20,957 web-based study activities have been completed using this informatics platform. Over 95% of respondents to an end-user satisfaction survey felt that the informatics platform was successful almost always or most of the time. The EPGP informatics platform has successfully and effectively allowed study management and efficient and reliable collection of phenotypic data. Our novel informatics platform met the requirements of a large, multicenter research project. The platform has had a high level of end-user acceptance by principal investigators and study coordinators, and can serve as a model for new tools to support future large scale, collaborative research projects collecting extensive phenotypic data. Copyright © 2012

Rhabdoviruses as vaccine platforms for infectious disease and cancer.

Science.gov (United States)

Zemp, Franz; Rajwani, Jahanara; Mahoney, Douglas J

2018-05-21

The family Rhabdoviridae (RV) comprises a large, genetically diverse collection of single-stranded, negative sense RNA viruses from the order Mononegavirales. Several RV members are being developed as live-attenuated vaccine vectors for the prevention or treatment of infectious disease and cancer. These include the prototype recombinant Vesicular Stomatitis Virus (rVSV) and the more recently developed recombinant Maraba Virus, both species within the genus Vesiculoviridae. A relatively strong safety profile in humans, robust immunogenicity and genetic malleability are key features that make the RV family attractive vaccine platforms. Currently, the rVSV vector is in preclinical development for vaccination against numerous high-priority infectious diseases, with clinical evaluation underway for HIV/AIDS and Ebola virus disease. Indeed, the success of the rVSV-ZEBOV vaccine during the 2014-15 Ebola virus outbreak in West Africa highlights the therapeutic potential of rVSV as a vaccine vector for acute, life-threatening viral illnesses. The rVSV and rMaraba platforms are also being tested as 'oncolytic' cancer vaccines in a series of phase 1-2 clinical trials, after being proven effective at eliciting immune-mediated tumour regression in preclinical mouse models. In this review, we discuss the biological and genetic features that make RVs attractive vaccine platforms and the development and ongoing testing of rVSV and rMaraba strains as vaccine vectors for infectious disease and cancer.

Platform dependence of inference on gene-wise and gene-set involvement in human lung development

Directory of Open Access Journals (Sweden)

Kho Alvin T

2009-06-01

Full Text Available Abstract Background With the recent development of microarray technologies, the comparability of gene expression data obtained from different platforms poses an important problem. We evaluated two widely used platforms, Affymetrix U133 Plus 2.0 and the Illumina HumanRef-8 v2 Expression Bead Chips, for comparability in a biological system in which changes may be subtle, namely fetal lung tissue as a function of gestational age. Results We performed the comparison via sequence-based probe matching between the two platforms. "Significance grouping" was defined as a measure of comparability. Using both expression correlation and significance grouping as measures of comparability, we demonstrated that despite overall cross-platform differences at the single gene level, increased correlation between the two platforms was found in genes with higher expression level, higher probe overlap, and lower p-value. We also demonstrated that biological function as determined via KEGG pathways or GO categories is more consistent across platforms than single gene analysis. Conclusion We conclude that while the comparability of the platforms at the single gene level may be increased by increasing sample size, they are highly comparable ontologically even for subtle differences in a relatively small sample size. Biologically relevant inference should therefore be reproducible across laboratories using different platforms.

Carbonate platform growth and demise offshore Central Vietnam

DEFF Research Database (Denmark)

Fyhn, Michael B.W.; Boldreel, Lars Ole; Nielsen, Lars H.

2013-01-01

Fault Zone, the Tuy Hoa Carbonate Platform fringes the continental margin between Da Nang and Nha Trang. Here, platform growth initiated during the Early Miocene and continued until Middle Miocene time when regional uplift led to subaerial exposure, termination of platform growth and karstification...... continues on isolated platforms hosting the Paracel Islands farther seawards. The onset of widespread carbonate deposition largely reflects the Early Miocene transgression of the area linked with early post-rift subsidence and the opening of the South China Sea. The mid-Neogene shift in carbonate deposition...

Towards A Framework of Digital Payment Platform Design

DEFF Research Database (Denmark)

Kazan, Erol; Damsgaard, Jan

This paper focuses on the triumph march of mobile phones that currently are annexing music players, navigation devices, and cameras as separate physical objects. The next target is set on payment. Through synthesizing available literature, we construct a framework for studying digital payment...... platforms that combines platform, technology and business design aspects. The framework is applied to conduct a comparative case study of digital payment platforms. Four types of market actors are considered: banks, mobile network operators, merchants, and startups, which are incumbents and disrupters....... By hosting third-party services, payment instruments are evolving from single-purpose to multi-functional ones. Our research extends existing payment literature from the MSP perspective to emphasize certain digital payment platform components, which impact strategies and complementary products....

Synthesis of Large-Scale Single-Crystalline Monolayer WS2 Using a Semi-Sealed Method

Directory of Open Access Journals (Sweden)

Feifei Lan

2018-02-01

Full Text Available As a two-dimensional semiconductor, WS2 has attracted great attention due to its rich physical properties and potential applications. However, it is still difficult to synthesize monolayer single-crystalline WS2 at larger scale. Here, we report the growth of large-scale triangular single-crystalline WS2 with a semi-sealed installation by chemical vapor deposition (CVD. Through this method, triangular single-crystalline WS2 with an average length of more than 300 µm was obtained. The largest one was about 405 μm in length. WS2 triangles with different sizes and thicknesses were analyzed by optical microscope and atomic force microscope (AFM. Their optical properties were evaluated by Raman and photoluminescence (PL spectra. This report paves the way to fabricating large-scale single-crystalline monolayer WS2, which is useful for the growth of high-quality WS2 and its potential applications in the future.

Large-core single-mode rib SU8 waveguide using solvent-assisted microcontact molding.

Science.gov (United States)

Huang, Cheng-Sheng; Wang, Wei-Chih

2008-09-01

This paper describes a novel fabrication technique for constructing a polymer-based large-core single-mode rib waveguide. A negative tone SU8 photoresist with a high optical transmission over a large wavelength range and stable mechanical properties was used as a waveguide material. A waveguide was constructed by using a polydimethylsiloxane stamp combined with a solvent-assisted microcontact molding technique. The effects on the final pattern's geometry of four different process conditions were investigated. Optical simulations were performed using beam propagation method software. Single-mode beam propagation was observed at the output of the simulated waveguide as well as the actual waveguide through the microscope image.

Design and performance of the virtualization platform for offline computing on the ATLAS TDAQ Farm

Science.gov (United States)

Ballestrero, S.; Batraneanu, S. M.; Brasolin, F.; Contescu, C.; Di Girolamo, A.; Lee, C. J.; Pozo Astigarraga, M. E.; Scannicchio, D. A.; Twomey, M. S.; Zaytsev, A.

2014-06-01

With the LHC collider at CERN currently going through the period of Long Shutdown 1 there is an opportunity to use the computing resources of the experiments' large trigger farms for other data processing activities. In the case of the ATLAS experiment, the TDAQ farm, consisting of more than 1500 compute nodes, is suitable for running Monte Carlo (MC) production jobs that are mostly CPU and not I/O bound. This contribution gives a thorough review of the design and deployment of a virtualized platform running on this computing resource and of its use to run large groups of CernVM based virtual machines operating as a single CERN-P1 WLCG site. This platform has been designed to guarantee the security and the usability of the ATLAS private network, and to minimize interference with TDAQ's usage of the farm. Openstack has been chosen to provide a cloud management layer. The experience gained in the last 3.5 months shows that the use of the TDAQ farm for the MC simulation contributes to the ATLAS data processing at the level of a large Tier-1 WLCG site, despite the opportunistic nature of the underlying computing resources being used.

Functional nanostructured platforms for chemical and biological sensing

Science.gov (United States)

Létant, S. E.

2006-05-01

The central goal of our work is to combine semiconductor nanotechnology and surface functionalization in order to build platforms for the selective detection of bio-organisms ranging in size from bacteria (micron range) down to viruses, as well as for the detection of chemical agents (nanometer range). We will show on three porous silicon platforms how pore geometry and pore wall chemistry can be combined and optimized to capture and detect specific targets. We developed a synthetic route allowing to directly anchor proteins on silicon surfaces and illustrated the relevance of this technique by immobilizing live enzymes onto electrochemically etched luminescent nano-porous silicon. The powerful association of the specific enzymes with the transducing matrix led to a selective hybrid platform for chemical sensing. We also used light-assisted electrochemistry to produce periodic arrays of through pores on pre-patterned silicon membranes with controlled diameters ranging from many microns down to tens of nanometers. We demonstrated the first covalently functionalized silicon membranes and illustrated their selective capture abilities with antibody-coated micro-beads. These engineered membranes are extremely versatile and could be adapted to specifically recognize the external fingerprints (size and coat composition) of target bio-organisms. Finally, we fabricated locally functionalized single nanopores using a combination of focused ion beam drilling and ion beam assisted oxide deposition. We showed how a silicon oxide ring can be grown around a single nanopore and how it can be functionalized with DNA probes to detect single viral-sized beads. The next step for this platform is the detection of whole viruses and bacteria.

Research status of large mode area single polarization active fiber

Science.gov (United States)

Xiao, Chun; Zhang, Ge; Yang, Bin-hua; Cheng, Wei-feng; Gu, Shao-yi

2018-03-01

As high power fiber laser used more and more widely, to increase the output power of fiber laser and beam quality improvement have become an important goal for the development of high power fiber lasers. The use of large mode fiber is the most direct and effective way to solve the nonlinear effect and fiber damage in the fiber laser power lifting process. In order to reduce the effect of polarization of the fiber laser system, the study found that when introduces a birefringence in the single-mode fiber, the polarization state changes caused by the birefringence is far greater than the random polarization state changes, then the external disturbance is completely submerged, finally the polarization can be controlled and stabilized. Through the fine design of the fiber structure, if the birefringence is high enough to achieve the separation of the two polarization states, the fiber will have a different cut-off mechanism to eliminate polarization which is not need, which will realize single mode single polarization transmission in a band. In this paper, different types of single polarization fiber design are presented and the application of these fibers are also discussed.

Infrared microspectroscopy: a multiple-screening platform for investigating single-cell biochemical perturbations upon prion infection.

Science.gov (United States)

Didonna, Alessandro; Vaccari, Lisa; Bek, Alpan; Legname, Giuseppe

2011-03-16

Prion diseases are a group of fatal neurodegenerative disorders characterized by the accumulation of prions in the central nervous system. The pathogenic prion (PrP(Sc)) possesses the capability to convert the host-encoded cellular isoform of the prion protein, PrP(C), into nascent PrP(Sc). The present work aims at providing novel insight into cellular response upon prion infection evidenced by synchrotron radiation infrared microspectroscopy (SR-IRMS). This non-invasive, label-free analytical technique was employed to investigate the biochemical perturbations undergone by prion infected mouse hypothalamic GT1-1 cells at the cellular and subcellular level. A decrement in total cellular protein content upon prion infection was identified by infrared (IR) whole-cell spectra and validated by bicinchoninic acid assay and single-cell volume analysis by atomic force microscopy (AFM). Hierarchical cluster analysis (HCA) of IR data discriminated between infected and uninfected cells and allowed to deduce an increment of lysosomal bodies within the cytoplasm of infected GT1-1 cells, a hypothesis further confirmed by SR-IRMS at subcellular spatial resolution and fluorescent microscopy. The purpose of this work, therefore, consists of proposing IRMS as a powerful multiscreening platform, drawing on the synergy with conventional biological assays and microscopy techniques in order to increase the accuracy of investigations performed at the single-cell level.

Infrared Microspectroscopy: A Multiple-Screening Platform for Investigating Single-Cell Biochemical Perturbations upon Prion Infection

Science.gov (United States)

2011-01-01

Prion diseases are a group of fatal neurodegenerative disorders characterized by the accumulation of prions in the central nervous system. The pathogenic prion (PrPSc) possesses the capability to convert the host-encoded cellular isoform of the prion protein, PrPC, into nascent PrPSc. The present work aims at providing novel insight into cellular response upon prion infection evidenced by synchrotron radiation infrared microspectroscopy (SR-IRMS). This non-invasive, label-free analytical technique was employed to investigate the biochemical perturbations undergone by prion infected mouse hypothalamic GT1-1 cells at the cellular and subcellular level. A decrement in total cellular protein content upon prion infection was identified by infrared (IR) whole-cell spectra and validated by bicinchoninic acid assay and single-cell volume analysis by atomic force microscopy (AFM). Hierarchical cluster analysis (HCA) of IR data discriminated between infected and uninfected cells and allowed to deduce an increment of lysosomal bodies within the cytoplasm of infected GT1-1 cells, a hypothesis further confirmed by SR-IRMS at subcellular spatial resolution and fluorescent microscopy. The purpose of this work, therefore, consists of proposing IRMS as a powerful multiscreening platform, drawing on the synergy with conventional biological assays and microscopy techniques in order to increase the accuracy of investigations performed at the single-cell level. PMID:22778865

Business and IT Capabilities for Cloud Platform Success

DEFF Research Database (Denmark)

Hahn, Christopher; Huntgeburth, Jan; Winkler, Till J.

2016-01-01

The growing proliferation of cloud platform ecosystems demands a deeper understanding of the capabilities that help existing and emerging platform providers to be successful by creating and appropriating value. This multiple case study of four cloud platform providers (three large, one SME......) instantiates Rai and Tang’s (2014) framework of dyadic IT and network IT capabilities for a cloud platform context and extends it by exploring previously undertheorized cloud platform business capabilities. We further build on this extended framework by employing a configurational perspective to elucidate...... the complementary role of the three proposed business capabilities (incentives and rules, ecosystem marketing and sales, partner development and support) for relevant value creation and appropriation mechanisms. In addition to providing a capability framework catered to the cloud platform context, our findings...

A truly Lego®-like modular microfluidics platform

Science.gov (United States)

Vittayarukskul, Kevin; Lee, Abraham Phillip

2017-03-01

Ideally, a modular microfluidics platform should be simple to assemble and support 3D configurations for increased versatility. The modular building blocks should also be mass producible like electrical components. These are fundamental features of world-renowned Legos® and why Legos® inspire many existing modular microfluidics platforms. In this paper, a truly Lego®-like microfluidics platform is introduced, and its basic feasibility is demonstrated. Here, PDMS building blocks resembling 2  ×  2 Lego® bricks are cast from 3D-printed master molds. The blocks are pegged and stacked on a traditional Lego® plate to create simple, 3D microfluidic networks, such as a single basket weave. Characteristics of the platform, including reversible sealing and automatic alignment of channels, are also analyzed and discussed in detail.

A truly Lego®-like modular microfluidics platform

International Nuclear Information System (INIS)

Vittayarukskul, Kevin; Lee, Abraham Phillip

2017-01-01

Ideally, a modular microfluidics platform should be simple to assemble and support 3D configurations for increased versatility. The modular building blocks should also be mass producible like electrical components. These are fundamental features of world-renowned Legos ® and why Legos ® inspire many existing modular microfluidics platforms. In this paper, a truly Lego ® -like microfluidics platform is introduced, and its basic feasibility is demonstrated. Here, PDMS building blocks resembling 2  ×  2 Lego ® bricks are cast from 3D-printed master molds. The blocks are pegged and stacked on a traditional Lego ® plate to create simple, 3D microfluidic networks, such as a single basket weave. Characteristics of the platform, including reversible sealing and automatic alignment of channels, are also analyzed and discussed in detail. (paper)

Anisotropic charge transport in large single crystals of π-conjugated organic molecules.

Science.gov (United States)

Hourani, Wael; Rahimi, Khosrow; Botiz, Ioan; Koch, Felix Peter Vinzenz; Reiter, Günter; Lienerth, Peter; Heiser, Thomas; Bubendorff, Jean-Luc; Simon, Laurent

2014-05-07

The electronic properties of organic semiconductors depend strongly on the nature of the molecules, their conjugation and conformation, their mutual distance and the orientation between adjacent molecules. Variations of intramolecular distances and conformation disturb the conjugation and perturb the delocalization of charges. As a result, the mobility considerably decreases compared to that of a covalently well-organized crystal. Here, we present electrical characterization of large single crystals made of the regioregular octamer of 3-hexyl-thiophene (3HT)8 using a conductive-atomic force microscope (C-AFM) in air. We find a large anisotropy in the conduction with charge mobility values depending on the crystallographic orientation of the single crystal. The smaller conduction is in the direction of π-π stacking (along the long axis of the single crystal) with a mobility value in the order of 10(-3) cm(2) V(-1) s(-1), and the larger one is along the molecular axis (in the direction normal to the single crystal surface) with a mobility value in the order of 0.5 cm(2) V(-1) s(-1). The measured current-voltage (I-V) curves showed that along the molecular axis, the current followed an exponential dependence corresponding to an injection mode. In the π-π stacking direction, the current exhibits a space charge limited current (SCLC) behavior, which allows us to estimate the charge carrier mobility.

The EU-ADR Web Platform: delivering advanced pharmacovigilance tools.

Science.gov (United States)

Oliveira, José Luis; Lopes, Pedro; Nunes, Tiago; Campos, David; Boyer, Scott; Ahlberg, Ernst; van Mulligen, Erik M; Kors, Jan A; Singh, Bharat; Furlong, Laura I; Sanz, Ferran; Bauer-Mehren, Anna; Carrascosa, Maria C; Mestres, Jordi; Avillach, Paul; Diallo, Gayo; Díaz Acedo, Carlos; van der Lei, Johan

2013-05-01

Pharmacovigilance methods have advanced greatly during the last decades, making post-market drug assessment an essential drug evaluation component. These methods mainly rely on the use of spontaneous reporting systems and health information databases to collect expertise from huge amounts of real-world reports. The EU-ADR Web Platform was built to further facilitate accessing, monitoring and exploring these data, enabling an in-depth analysis of adverse drug reactions risks. The EU-ADR Web Platform exploits the wealth of data collected within a large-scale European initiative, the EU-ADR project. Millions of electronic health records, provided by national health agencies, are mined for specific drug events, which are correlated with literature, protein and pathway data, resulting in a rich drug-event dataset. Next, advanced distributed computing methods are tailored to coordinate the execution of data-mining and statistical analysis tasks. This permits obtaining a ranked drug-event list, removing spurious entries and highlighting relationships with high risk potential. The EU-ADR Web Platform is an open workspace for the integrated analysis of pharmacovigilance datasets. Using this software, researchers can access a variety of tools provided by distinct partners in a single centralized environment. Besides performing standalone drug-event assessments, they can also control the pipeline for an improved batch analysis of custom datasets. Drug-event pairs can be substantiated and statistically analysed within the platform's innovative working environment. A pioneering workspace that helps in explaining the biological path of adverse drug reactions was developed within the EU-ADR project consortium. This tool, targeted at the pharmacovigilance community, is available online at https://bioinformatics.ua.pt/euadr/. Copyright © 2012 John Wiley & Sons, Ltd.

Multidimensional quantum entanglement with large-scale integrated optics

DEFF Research Database (Denmark)

Wang, Jianwei; Paesani, Stefano; Ding, Yunhong

2018-01-01

-dimensional entanglement. A programmable bipartite entangled system is realized with dimension up to 15 × 15 on a large-scale silicon-photonics quantum circuit. The device integrates more than 550 photonic components on a single chip, including 16 identical photon-pair sources. We verify the high precision, generality......The ability to control multidimensional quantum systems is key for the investigation of fundamental science and for the development of advanced quantum technologies. We demonstrate a multidimensional integrated quantum photonic platform able to generate, control and analyze high...

Online Model Evaluation in a Large-Scale Computational Advertising Platform

OpenAIRE

Shariat, Shahriar; Orten, Burkay; Dasdan, Ali

2015-01-01

Online media provides opportunities for marketers through which they can deliver effective brand messages to a wide range of audiences. Advertising technology platforms enable advertisers to reach their target audience by delivering ad impressions to online users in real time. In order to identify the best marketing message for a user and to purchase impressions at the right price, we rely heavily on bid prediction and optimization models. Even though the bid prediction models are well studie...

A combined electrochemical and optical trapping platform for measuring single cell respiration rates at electrode interfaces

Energy Technology Data Exchange (ETDEWEB)

Gross, Benjamin J. [Department of Physics and Astronomy, University of Southern California, 920 Bloom Walk, Los Angeles, California 90089-0484 (United States); El-Naggar, Mohamed Y., E-mail: mnaggar@usc.edu [Department of Physics and Astronomy, University of Southern California, 920 Bloom Walk, Los Angeles, California 90089-0484 (United States); Molecular and Computational Biology Section, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089-0484 (United States); Department of Chemistry, University of Southern California, Los Angeles, California 90089-0484 (United States)

2015-06-15

Metal-reducing bacteria gain energy by extracellular electron transfer to external solids, such as naturally abundant minerals, which substitute for oxygen or the other common soluble electron acceptors of respiration. This process is one of the earliest forms of respiration on earth and has significant environmental and technological implications. By performing electron transfer to electrodes instead of minerals, these microbes can be used as biocatalysts for conversion of diverse chemical fuels to electricity. Understanding such a complex biotic-abiotic interaction necessitates the development of tools capable of probing extracellular electron transfer down to the level of single cells. Here, we describe an experimental platform for single cell respiration measurements. The design integrates an infrared optical trap, perfusion chamber, and lithographically fabricated electrochemical chips containing potentiostatically controlled transparent indium tin oxide microelectrodes. Individual bacteria are manipulated using the optical trap and placed on the microelectrodes, which are biased at a suitable oxidizing potential in the absence of any chemical electron acceptor. The potentiostat is used to detect the respiration current correlated with cell-electrode contact. We demonstrate the system with single cell measurements of the dissimilatory-metal reducing bacterium Shewanella oneidensis MR-1, which resulted in respiration currents ranging from 15 fA to 100 fA per cell under our measurement conditions. Mutants lacking the outer-membrane cytochromes necessary for extracellular respiration did not result in any measurable current output upon contact. In addition to the application for extracellular electron transfer studies, the ability to electronically measure cell-specific respiration rates may provide answers for a variety of fundamental microbial physiology questions.

A combined electrochemical and optical trapping platform for measuring single cell respiration rates at electrode interfaces.

Science.gov (United States)

Gross, Benjamin J; El-Naggar, Mohamed Y

2015-06-01

Metal-reducing bacteria gain energy by extracellular electron transfer to external solids, such as naturally abundant minerals, which substitute for oxygen or the other common soluble electron acceptors of respiration. This process is one of the earliest forms of respiration on earth and has significant environmental and technological implications. By performing electron transfer to electrodes instead of minerals, these microbes can be used as biocatalysts for conversion of diverse chemical fuels to electricity. Understanding such a complex biotic-abiotic interaction necessitates the development of tools capable of probing extracellular electron transfer down to the level of single cells. Here, we describe an experimental platform for single cell respiration measurements. The design integrates an infrared optical trap, perfusion chamber, and lithographically fabricated electrochemical chips containing potentiostatically controlled transparent indium tin oxide microelectrodes. Individual bacteria are manipulated using the optical trap and placed on the microelectrodes, which are biased at a suitable oxidizing potential in the absence of any chemical electron acceptor. The potentiostat is used to detect the respiration current correlated with cell-electrode contact. We demonstrate the system with single cell measurements of the dissimilatory-metal reducing bacterium Shewanella oneidensis MR-1, which resulted in respiration currents ranging from 15 fA to 100 fA per cell under our measurement conditions. Mutants lacking the outer-membrane cytochromes necessary for extracellular respiration did not result in any measurable current output upon contact. In addition to the application for extracellular electron transfer studies, the ability to electronically measure cell-specific respiration rates may provide answers for a variety of fundamental microbial physiology questions.

A combined electrochemical and optical trapping platform for measuring single cell respiration rates at electrode interfaces

International Nuclear Information System (INIS)

Gross, Benjamin J.; El-Naggar, Mohamed Y.

2015-01-01

Metal-reducing bacteria gain energy by extracellular electron transfer to external solids, such as naturally abundant minerals, which substitute for oxygen or the other common soluble electron acceptors of respiration. This process is one of the earliest forms of respiration on earth and has significant environmental and technological implications. By performing electron transfer to electrodes instead of minerals, these microbes can be used as biocatalysts for conversion of diverse chemical fuels to electricity. Understanding such a complex biotic-abiotic interaction necessitates the development of tools capable of probing extracellular electron transfer down to the level of single cells. Here, we describe an experimental platform for single cell respiration measurements. The design integrates an infrared optical trap, perfusion chamber, and lithographically fabricated electrochemical chips containing potentiostatically controlled transparent indium tin oxide microelectrodes. Individual bacteria are manipulated using the optical trap and placed on the microelectrodes, which are biased at a suitable oxidizing potential in the absence of any chemical electron acceptor. The potentiostat is used to detect the respiration current correlated with cell-electrode contact. We demonstrate the system with single cell measurements of the dissimilatory-metal reducing bacterium Shewanella oneidensis MR-1, which resulted in respiration currents ranging from 15 fA to 100 fA per cell under our measurement conditions. Mutants lacking the outer-membrane cytochromes necessary for extracellular respiration did not result in any measurable current output upon contact. In addition to the application for extracellular electron transfer studies, the ability to electronically measure cell-specific respiration rates may provide answers for a variety of fundamental microbial physiology questions

Integrated System Design for a Large Wind Turbine Supported on a Moored Semi-Submersible Platform

Directory of Open Access Journals (Sweden)

Jinsong Liu

2018-01-01

Full Text Available Over the past few decades, wind energy has emerged as an alternative to conventional power generation that is economical, environmentally friendly and, importantly, renewable. Specifically, offshore wind energy is being considered by a number of countries to harness the stronger and more consistent wind resource compared to that over land. To meet the projected “20% energy from wind by 2030” scenario that was announced in 2006, 54 GW of added wind energy capacity need to come from offshore according to a National Renewable Energy Laboratory (NREL study. In this study, we discuss the development of a semi-submersible floating offshore platform with a catenary mooring system to support a very large 13.2-MW wind turbine with 100-m blades. An iterative design process is applied to baseline models with Froude scaling in order to achieve preliminary static stability. Structural dynamic analyses are performed to investigate the performance of the new model using a finite element method approach for the tower and a boundary integral equation (panel method for the platform. The steady-state response of the system under uniform wind and regular waves is first studied to evaluate the performance of the integrated system. Response amplitude operators (RAOs are computed in the time domain using white-noise wave excitation; this serves to highlight nonlinear, as well as dynamic characteristics of the system. Finally, selected design load cases (DLCs and the stochastic dynamic response of the system are studied to assess the global performance for sea states defined by wind fields with turbulence and long-crested irregular waves.

Bionimbus: a cloud for managing, analyzing and sharing large genomics datasets.

Science.gov (United States)

Heath, Allison P; Greenway, Matthew; Powell, Raymond; Spring, Jonathan; Suarez, Rafael; Hanley, David; Bandlamudi, Chai; McNerney, Megan E; White, Kevin P; Grossman, Robert L

2014-01-01

As large genomics and phenotypic datasets are becoming more common, it is increasingly difficult for most researchers to access, manage, and analyze them. One possible approach is to provide the research community with several petabyte-scale cloud-based computing platforms containing these data, along with tools and resources to analyze it. Bionimbus is an open source cloud-computing platform that is based primarily upon OpenStack, which manages on-demand virtual machines that provide the required computational resources, and GlusterFS, which is a high-performance clustered file system. Bionimbus also includes Tukey, which is a portal, and associated middleware that provides a single entry point and a single sign on for the various Bionimbus resources; and Yates, which automates the installation, configuration, and maintenance of the software infrastructure required. Bionimbus is used by a variety of projects to process genomics and phenotypic data. For example, it is used by an acute myeloid leukemia resequencing project at the University of Chicago. The project requires several computational pipelines, including pipelines for quality control, alignment, variant calling, and annotation. For each sample, the alignment step requires eight CPUs for about 12 h. BAM file sizes ranged from 5 GB to 10 GB for each sample. Most members of the research community have difficulty downloading large genomics datasets and obtaining sufficient storage and computer resources to manage and analyze the data. Cloud computing platforms, such as Bionimbus, with data commons that contain large genomics datasets, are one choice for broadening access to research data in genomics. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Design and Performance of the Virtualization Platform for Offline computing on the ATLAS TDAQ Farm

CERN Document Server

Ballestrero, S; The ATLAS collaboration; Brasolin, F; Contescu, C; Di Girolamo, A; Lee, C J; Pozo Astigarraga, M E; Scannicchio, D A; Twomey, M S; Zaytsev, A

2013-01-01

With the LHC collider at CERN currently going through the period of Long Shutdown 1 (LS1) there is a remarkable opportunity to use the computing resources of the large trigger farms of the experiments for other data processing activities. In the case of ATLAS experiment the TDAQ farm, consisting of more than 1500 compute nodes, is particularly suitable for running Monte Carlo production jobs that are mostly CPU and not I/O bound. This contribution gives a thorough review of all the stages of Sim@P1 project dedicated to the design and deployment of a virtualized platform running on the ATLAS TDAQ computing resources and using it to run the large groups of CernVM based virtual machines operating as a single CERN-P1 WLCG site. This platform has been designed to avoid interference with TDAQ usage of the farm and to guarantee the security and the usability of the ATLAS private network; Openstack has been chosen to provide a cloud management layer. The approaches to organizing support for the sustained operation of...

Design and Performance of the Virtualization Platform for Offline computing on the ATLAS TDAQ Farm

CERN Document Server

Ballestrero, S; The ATLAS collaboration; Brasolin, F; Contescu, C; Di Girolamo, A; Lee, C J; Pozo Astigarraga, M E; Scannicchio, D A; Twomey, M S; Zaytsev, A

2014-01-01

With the LHC collider at CERN currently going through the period of Long Shutdown 1 (LS1) there is a remarkable opportunity to use the computing resources of the large trigger farms of the experiments for other data processing activities. In the case of ATLAS experiment the TDAQ farm, consisting of more than 1500 compute nodes, is particularly suitable for running Monte Carlo production jobs that are mostly CPU and not I/O bound. This contribution gives a thorough review of all the stages of Sim@P1 project dedicated to the design and deployment of a virtualized platform running on the ATLAS TDAQ computing resources and using it to run the large groups of CernVM based virtual machines operating as a single CERN-P1 WLCG site. This platform has been designed to avoid interference with TDAQ usage of the farm and to guarantee the security and the usability of the ATLAS private network; Openstack has been chosen to provide a cloud management layer. The approaches to organizing support for the sustained operation of...

Parasitic neutron bragg reflections from large imperfect single crystals

Energy Technology Data Exchange (ETDEWEB)

Naguib, K.; Adib, M

1998-12-01

A formula is given which allows to calculate the contribution of the total Bragg scattering from different (hkl) planes to the neutron transmission through a large imperfect single crystals. The formula takes into account the crystal structure type, its mosaic spread value, the plane along which the crystal surface is cut along and its orientation with respect to the neutron beam direction. A computer program ISCANF-1 was developed to calculate the total parasitic scattering cross-section from different (hkl) planes as well as the nuclear and diffuse scattering cross-sections. The ISCANF-1 program was applied to calculate the neutron attenuation through Cu and Zn single crystals, each of them cut along (002) planes. The calculated values of the neutron transmission through Cu and Zn crystals were compared with the measured ones in the wavelength range 0.21-0.47 nm and 0.04-0.52 nm respectively. The measured and calculated values were found to be in reasonable agreement within the statistical accuracy. The computer program ISCANF-1 was also applied to investigate the effect of parasitic Bragg scattering on the neutron filtering characteristics of both Zn and Cu single crystals as a function of their physical parameters.

Parasitic neutron bragg reflections from large imperfect single crystals

International Nuclear Information System (INIS)

Naguib, K.; Adib, M.

1998-01-01

A formula is given which allows to calculate the contribution of the total Bragg scattering from different (hkl) planes to the neutron transmission through a large imperfect single crystals. The formula takes into account the crystal structure type, its mosaic spread value, the plane along which the crystal surface is cut along and its orientation with respect to the neutron beam direction. A computer program ISCANF-1 was developed to calculate the total parasitic scattering cross-section from different (hkl) planes as well as the nuclear and diffuse scattering cross-sections. The ISCANF-1 program was applied to calculate the neutron attenuation through Cu and Zn single crystals, each of them cut along (002) planes. The calculated values of the neutron transmission through Cu and Zn crystals were compared with the measured ones in the wavelength range 0.21-0.47 nm and 0.04-0.52 nm respectively. The measured and calculated values were found to be in reasonable agreement within the statistical accuracy. The computer program ISCANF-1 was also applied to investigate the effect of parasitic Bragg scattering on the neutron filtering characteristics of both Zn and Cu single crystals as a function of their physical parameters

Control Algorithms for Large-scale Single-axis Photovoltaic Trackers

Directory of Open Access Journals (Sweden)

Dorian Schneider

2012-01-01

Full Text Available The electrical yield of large-scale photovoltaic power plants can be greatly improved by employing solar trackers. While fixed-tilt superstructures are stationary and immobile, trackers move the PV-module plane in order to optimize its alignment to the sun. This paper introduces control algorithms for single-axis trackers (SAT, including a discussion for optimal alignment and backtracking. The results are used to simulate and compare the electrical yield of fixed-tilt and SAT systems. The proposed algorithms have been field tested, and are in operation in solar parks worldwide.

HPC - Platforms Penta Chart

Energy Technology Data Exchange (ETDEWEB)

Trujillo, Angelina Michelle [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

2015-10-08

Strategy, Planning, Acquiring- very large scale computing platforms come and go and planning for immensely scalable machines often precedes actual procurement by 3 years. Procurement can be another year or more. Integration- After Acquisition, machines must be integrated into the computing environments at LANL. Connection to scalable storage via large scale storage networking, assuring correct and secure operations. Management and Utilization – Ongoing operations, maintenance, and trouble shooting of the hardware and systems software at massive scale is required.

Single Crystal Piezomotor for Large Stroke, High Precision and Cryogenic Actuations, Phase I

Data.gov (United States)

National Aeronautics and Space Administration — TRS Technologies proposes a novel single crystal piezomotor for large stroke, high precision, and cryogenic actuations with capability of position set-hold with...

Professional Cross-Platform Mobile Development in C#

CERN Document Server

Olson, Scott; Horgen, Ben; Goers, Kenny

2012-01-01

Develop mobile enterprise applications in a language you already know! With employees, rather than the IT department, now driving the decision of which devices to use on the job, many companies are scrambling to integrate enterprise applications. Fortunately, enterprise developers can now create apps for all major mobile devices using C#/.NET and Mono, languages most already know. A team of authors draws on their vast experiences to teach you how to create cross-platform mobile applications, while delivering the same functionality to PC's, laptops and the web from a single technology platform

Embedded-Based Graphics Processing Unit Cluster Platform for Multiple Sequence Alignments

Directory of Open Access Journals (Sweden)

Jyh-Da Wei

2017-08-01

Full Text Available High-end graphics processing units (GPUs, such as NVIDIA Tesla/Fermi/Kepler series cards with thousands of cores per chip, are widely applied to high-performance computing fields in a decade. These desktop GPU cards should be installed in personal computers/servers with desktop CPUs, and the cost and power consumption of constructing a GPU cluster platform are very high. In recent years, NVIDIA releases an embedded board, called Jetson Tegra K1 (TK1, which contains 4 ARM Cortex-A15 CPUs and 192 Compute Unified Device Architecture cores (belong to Kepler GPUs. Jetson Tegra K1 has several advantages, such as the low cost, low power consumption, and high applicability, and it has been applied into several specific applications. In our previous work, a bioinformatics platform with a single TK1 (STK platform was constructed, and this previous work is also used to prove that the Web and mobile services can be implemented in the STK platform with a good cost-performance ratio by comparing a STK platform with the desktop CPU and GPU. In this work, an embedded-based GPU cluster platform will be constructed with multiple TK1s (MTK platform. Complex system installation and setup are necessary procedures at first. Then, 2 job assignment modes are designed for the MTK platform to provide services for users. Finally, ClustalW v2.0.11 and ClustalWtk will be ported to the MTK platform. The experimental results showed that the speedup ratios achieved 5.5 and 4.8 times for ClustalW v2.0.11 and ClustalWtk, respectively, by comparing 6 TK1s with a single TK1. The MTK platform is proven to be useful for multiple sequence alignments.

Embedded-Based Graphics Processing Unit Cluster Platform for Multiple Sequence Alignments.

Science.gov (United States)

Wei, Jyh-Da; Cheng, Hui-Jun; Lin, Chun-Yuan; Ye, Jin; Yeh, Kuan-Yu

2017-01-01

High-end graphics processing units (GPUs), such as NVIDIA Tesla/Fermi/Kepler series cards with thousands of cores per chip, are widely applied to high-performance computing fields in a decade. These desktop GPU cards should be installed in personal computers/servers with desktop CPUs, and the cost and power consumption of constructing a GPU cluster platform are very high. In recent years, NVIDIA releases an embedded board, called Jetson Tegra K1 (TK1), which contains 4 ARM Cortex-A15 CPUs and 192 Compute Unified Device Architecture cores (belong to Kepler GPUs). Jetson Tegra K1 has several advantages, such as the low cost, low power consumption, and high applicability, and it has been applied into several specific applications. In our previous work, a bioinformatics platform with a single TK1 (STK platform) was constructed, and this previous work is also used to prove that the Web and mobile services can be implemented in the STK platform with a good cost-performance ratio by comparing a STK platform with the desktop CPU and GPU. In this work, an embedded-based GPU cluster platform will be constructed with multiple TK1s (MTK platform). Complex system installation and setup are necessary procedures at first. Then, 2 job assignment modes are designed for the MTK platform to provide services for users. Finally, ClustalW v2.0.11 and ClustalWtk will be ported to the MTK platform. The experimental results showed that the speedup ratios achieved 5.5 and 4.8 times for ClustalW v2.0.11 and ClustalWtk, respectively, by comparing 6 TK1s with a single TK1. The MTK platform is proven to be useful for multiple sequence alignments.

GraMi: Generalized Frequent Pattern Mining in a Single Large Graph

KAUST Repository

Saeedy, Mohammed El

2011-11-01

Mining frequent subgraphs is an important operation on graphs. Most existing work assumes a database of many small graphs, but modern applications, such as social networks, citation graphs or protein-protein interaction in bioinformatics, are modeled as a single large graph. Interesting interactions in such applications may be transitive (e.g., friend of a friend). Existing methods, however, search for frequent isomorphic (i.e., exact match) subgraphs and cannot discover many useful patterns. In this paper the authors propose GRAMI, a framework that generalizes frequent subgraph mining in a large single graph. GRAMI discovers frequent patterns. A pattern is a graph where edges are generalized to distance-constrained paths. Depending on the definition of the distance function, many instantiations of the framework are possible. Both directed and undirected graphs, as well as multiple labels per vertex, are supported. The authors developed an efficient implementation of the framework that models the frequency resolution phase as a constraint satisfaction problem, in order to avoid the costly enumeration of all instances of each pattern in the graph. The authors also implemented CGRAMI, a version that supports structural and semantic constraints; and AGRAMI, an approximate version that supports very large graphs. The experiments on real data demonstrate that the authors framework is up to 3 orders of magnitude faster and discovers more interesting patterns than existing approaches.

Helicopter flight simulation motion platform requirements

Science.gov (United States)

Schroeder, Jeffery Allyn

Flight simulators attempt to reproduce in-flight pilot-vehicle behavior on the ground. This reproduction is challenging for helicopter simulators, as the pilot is often inextricably dependent on external cues for pilot-vehicle stabilization. One important simulator cue is platform motion; however, its required fidelity is unknown. To determine the required motion fidelity, several unique experiments were performed. A large displacement motion platform was used that allowed pilots to fly tasks with matched motion and visual cues. Then, the platform motion was modified to give cues varying from full motion to no motion. Several key results were found. First, lateral and vertical translational platform cues had significant effects on fidelity. Their presence improved performance and reduced pilot workload. Second, yaw and roll rotational platform cues were not as important as the translational platform cues. In particular, the yaw rotational motion platform cue did not appear at all useful in improving performance or reducing workload. Third, when the lateral translational platform cue was combined with visual yaw rotational cues, pilots believed the platform was rotating when it was not. Thus, simulator systems can be made more efficient by proper combination of platform and visual cues. Fourth, motion fidelity specifications were revised that now provide simulator users with a better prediction of motion fidelity based upon the frequency responses of their motion control laws. Fifth, vertical platform motion affected pilot estimates of steady-state altitude during altitude repositionings. This refutes the view that pilots estimate altitude and altitude rate in simulation solely from visual cues. Finally, the combined results led to a general method for configuring helicopter motion systems and for developing simulator tasks that more likely represent actual flight. The overall results can serve as a guide to future simulator designers and to today's operators.

Development and application of an optogenetic platform for controlling and imaging a large number of individual neurons

Science.gov (United States)

Mohammed, Ali Ibrahim Ali

The understanding and treatment of brain disorders as well as the development of intelligent machines is hampered by the lack of knowledge of how the brain fundamentally functions. Over the past century, we have learned much about how individual neurons and neural networks behave, however new tools are critically needed to interrogate how neural networks give rise to complex brain processes and disease conditions. Recent innovations in molecular techniques, such as optogenetics, have enabled neuroscientists unprecedented precision to excite, inhibit and record defined neurons. The impressive sensitivity of currently available optogenetic sensors and actuators has now enabled the possibility of analyzing a large number of individual neurons in the brains of behaving animals. To promote the use of these optogenetic tools, this thesis integrates cutting edge optogenetic molecular sensors which is ultrasensitive for imaging neuronal activity with custom wide field optical microscope to analyze a large number of individual neurons in living brains. Wide-field microscopy provides a large field of view and better spatial resolution approaching the Abbe diffraction limit of fluorescent microscope. To demonstrate the advantages of this optical platform, we imaged a deep brain structure, the Hippocampus, and tracked hundreds of neurons over time while mouse was performing a memory task to investigate how those individual neurons related to behavior. In addition, we tested our optical platform in investigating transient neural network changes upon mechanical perturbation related to blast injuries. In this experiment, all blasted mice show a consistent change in neural network. A small portion of neurons showed a sustained calcium increase for an extended period of time, whereas the majority lost their activities. Finally, using optogenetic silencer to control selective motor cortex neurons, we examined their contributions to the network pathology of basal ganglia related to

Synthesis and structure of large single crystalline silver hexagonal microplates suitable for micromachining

Energy Technology Data Exchange (ETDEWEB)

Lyutov, Dimitar L.; Genkov, Kaloyan V.; Zyapkov, Anton D.; Tsutsumanova, Gichka G.; Tzonev, Atanas N. [Department of Solid State Physics and Microelectronics, Faculty of Physics, University of Sofia, 5, J. Bouchier Blvd, Sofia (Bulgaria); Lyutov, Lyudmil G. [Department of General and Inorganic Chemistry, Faculty of Chemistry, University of Sofia, 1, J. Bouchier Blvd, Sofia (Bulgaria); Russev, Stoyan C., E-mail: scr@phys.uni-sofia.bg [Department of Solid State Physics and Microelectronics, Faculty of Physics, University of Sofia, 5, J. Bouchier Blvd, Sofia (Bulgaria)

2014-01-15

We report a simple one-step synthesis method of large single crystalline Ag (111) hexagonal microplates with sharp edges and a size of up to tens of microns. Single silver crystals were produced by reduction silver nitrate aqueous solution with 4-(methylamino)phenol sulfate. Scanning and transmission electron microscopy, energy-dispersive X-ray spectroscopy, selected area electron diffraction and optical microscopy techniques were combined to characterize the crystals. It is shown that the microplates can be easily dispersed and transferred as single objects onto different substrates and subsequently used as a high quality plasmonic starting material for micromachining of future nanocomponents, using modern top-down techniques like focused-ion beam milling and gas injection deposition. - Highlights: • Synthesis of large Ag hexagonal microplates with high crystallinity. • It is shown and discussed the role of twinning for the anisotropic 2D growth. • The Ag plates are stable in water and can be dispersed onto different substrates. • Their positioning and subsequent micromachining with FIB/GIS is demonstrated. • Suitable starting material for future plasmonic nanocomponents.

Design and performance of the virtualization platform for offline computing on the ATLAS TDAQ Farm

International Nuclear Information System (INIS)

Ballestrero, S; Lee, C J; Batraneanu, S M; Scannicchio, D A; Brasolin, F; Contescu, C; Girolamo, A Di; Astigarraga, M E Pozo; Twomey, M S; Zaytsev, A

2014-01-01

With the LHC collider at CERN currently going through the period of Long Shutdown 1 there is an opportunity to use the computing resources of the experiments' large trigger farms for other data processing activities. In the case of the ATLAS experiment, the TDAQ farm, consisting of more than 1500 compute nodes, is suitable for running Monte Carlo (MC) production jobs that are mostly CPU and not I/O bound. This contribution gives a thorough review of the design and deployment of a virtualized platform running on this computing resource and of its use to run large groups of CernVM based virtual machines operating as a single CERN-P1 WLCG site. This platform has been designed to guarantee the security and the usability of the ATLAS private network, and to minimize interference with TDAQ's usage of the farm. Openstack has been chosen to provide a cloud management layer. The experience gained in the last 3.5 months shows that the use of the TDAQ farm for the MC simulation contributes to the ATLAS data processing at the level of a large Tier-1 WLCG site, despite the opportunistic nature of the underlying computing resources being used.

Statistical analysis of error rate of large-scale single flux quantum logic circuit by considering fluctuation of timing parameters

International Nuclear Information System (INIS)

Yamanashi, Yuki; Masubuchi, Kota; Yoshikawa, Nobuyuki

2016-01-01

The relationship between the timing margin and the error rate of the large-scale single flux quantum logic circuits is quantitatively investigated to establish a timing design guideline. We observed that the fluctuation in the set-up/hold time of single flux quantum logic gates caused by thermal noises is the most probable origin of the logical error of the large-scale single flux quantum circuit. The appropriate timing margin for stable operation of the large-scale logic circuit is discussed by taking the fluctuation of setup/hold time and the timing jitter in the single flux quantum circuits. As a case study, the dependence of the error rate of the 1-million-bit single flux quantum shift register on the timing margin is statistically analyzed. The result indicates that adjustment of timing margin and the bias voltage is important for stable operation of a large-scale SFQ logic circuit.

Statistical analysis of error rate of large-scale single flux quantum logic circuit by considering fluctuation of timing parameters

Energy Technology Data Exchange (ETDEWEB)

Yamanashi, Yuki, E-mail: yamanasi@ynu.ac.jp [Department of Electrical and Computer Engineering, Yokohama National University, Tokiwadai 79-5, Hodogaya-ku, Yokohama 240-8501 (Japan); Masubuchi, Kota; Yoshikawa, Nobuyuki [Department of Electrical and Computer Engineering, Yokohama National University, Tokiwadai 79-5, Hodogaya-ku, Yokohama 240-8501 (Japan)

2016-11-15

The relationship between the timing margin and the error rate of the large-scale single flux quantum logic circuits is quantitatively investigated to establish a timing design guideline. We observed that the fluctuation in the set-up/hold time of single flux quantum logic gates caused by thermal noises is the most probable origin of the logical error of the large-scale single flux quantum circuit. The appropriate timing margin for stable operation of the large-scale logic circuit is discussed by taking the fluctuation of setup/hold time and the timing jitter in the single flux quantum circuits. As a case study, the dependence of the error rate of the 1-million-bit single flux quantum shift register on the timing margin is statistically analyzed. The result indicates that adjustment of timing margin and the bias voltage is important for stable operation of a large-scale SFQ logic circuit.

HipMatch: an object-oriented cross-platform program for accurate determination of cup orientation using 2D-3D registration of single standard X-ray radiograph and a CT volume.

Science.gov (United States)

Zheng, Guoyan; Zhang, Xuan; Steppacher, Simon D; Murphy, Stephen B; Siebenrock, Klaus A; Tannast, Moritz

2009-09-01

The widely used procedure of evaluation of cup orientation following total hip arthroplasty using single standard anteroposterior (AP) radiograph is known inaccurate, largely due to the wide variability in individual pelvic orientation relative to X-ray plate. 2D-3D image registration methods have been introduced for an accurate determination of the post-operative cup alignment with respect to an anatomical reference extracted from the CT data. Although encouraging results have been reported, their extensive usage in clinical routine is still limited. This may be explained by their requirement of a CAD model of the prosthesis, which is often difficult to be organized from the manufacturer due to the proprietary issue, and by their requirement of either multiple radiographs or a radiograph-specific calibration, both of which are not available for most retrospective studies. To address these issues, we developed and validated an object-oriented cross-platform program called "HipMatch" where a hybrid 2D-3D registration scheme combining an iterative landmark-to-ray registration with a 2D-3D intensity-based registration was implemented to estimate a rigid transformation between a pre-operative CT volume and the post-operative X-ray radiograph for a precise estimation of cup alignment. No CAD model of the prosthesis is required. Quantitative and qualitative results evaluated on cadaveric and clinical datasets are given, which indicate the robustness and the accuracy of the program. HipMatch is written in object-oriented programming language C++ using cross-platform software Qt (TrollTech, Oslo, Norway), VTK, and Coin3D and is transportable to any platform.

Multidimensional quantum entanglement with large-scale integrated optics.

Science.gov (United States)

Wang, Jianwei; Paesani, Stefano; Ding, Yunhong; Santagati, Raffaele; Skrzypczyk, Paul; Salavrakos, Alexia; Tura, Jordi; Augusiak, Remigiusz; Mančinska, Laura; Bacco, Davide; Bonneau, Damien; Silverstone, Joshua W; Gong, Qihuang; Acín, Antonio; Rottwitt, Karsten; Oxenløwe, Leif K; O'Brien, Jeremy L; Laing, Anthony; Thompson, Mark G

2018-04-20

The ability to control multidimensional quantum systems is central to the development of advanced quantum technologies. We demonstrate a multidimensional integrated quantum photonic platform able to generate, control, and analyze high-dimensional entanglement. A programmable bipartite entangled system is realized with dimensions up to 15 × 15 on a large-scale silicon photonics quantum circuit. The device integrates more than 550 photonic components on a single chip, including 16 identical photon-pair sources. We verify the high precision, generality, and controllability of our multidimensional technology, and further exploit these abilities to demonstrate previously unexplored quantum applications, such as quantum randomness expansion and self-testing on multidimensional states. Our work provides an experimental platform for the development of multidimensional quantum technologies. Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

Single incision pediatric endoscopic surgery: advantages of relatively large incision

International Nuclear Information System (INIS)

Yilmaz, E.; Afsarlar, E.; Karaman, I.

2015-01-01

To describe Single Incision Pediatric Endoscopic Surgery (SIPES) performed on children with various diagnoses, emphasizing its advantages. Study Design: An observational case series. Place and Duration of Study: Department of Pediatric Surgery, Dr. Sami Ulus Maternity and Child Health Hospital, Ankara, Turkey, from January 2011 to November 2014. Methodology: A review of patient charts was conducted in which SIPES was preferred as the surgical procedure. Patient demographics, operative details, operative time, clinical outcomes, postoperative pain and cosmesis were analyzed. Results: SIPES was performed on 45 patients (21 girls, 24 boys). Thirty-three appendectomies, 5 varicocelectomies, 3 oophorectomies, 2 ovarian and one paratubal cyst excision, and one fallopian tube excision were performed. All except one procedures were performed through our standard 2cm umbilical vertical or smile incision. In 18 cases, abdominal irrigation/aspiration was easily performed through the existing larger incision, as is done with open surgical technique. None of the patients had early postoperative shoulder/back pain since complete disinflation of CO/sub 2/ could be ensured. All of the patients/parents were satisfied with the cosmesis. Conclusion: SIPES has the advantages of limiting the surgical scar to within the umbilicus and providing easy disinflation of CO/sub 2/, allowing intraabdominal cleaning and extraction of large volume tissue samples through a single large umbilical incision. (author)

Rigid multipodal platforms for metal surfaces

Directory of Open Access Journals (Sweden)

Michal Valášek

2016-03-01

Full Text Available In this review the recent progress in molecular platforms that form rigid and well-defined contact to a metal surface are discussed. Most of the presented examples have at least three anchoring units in order to control the spatial arrangement of the protruding molecular subunit. Another interesting feature is the lateral orientation of these foot structures which, depending on the particular application, is equally important as the spatial arrangement of the molecules. The numerous approaches towards assembling and organizing functional molecules into specific architectures on metal substrates are reviewed here. Particular attention is paid to variations of both, the core structures and the anchoring groups. Furthermore, the analytical methods enabling the investigation of individual molecules as well as monomolecular layers of ordered platform structures are summarized. The presented multipodal platforms bearing several anchoring groups form considerably more stable molecule–metal contacts than corresponding monopodal analogues and exhibit an enlarged separation of the functional molecules due to the increased footprint, as well as restrict tilting of the functional termini with respect to the metal surface. These platforms are thus ideally suited to tune important properties of the molecule–metal interface. On a single-molecule level, several of these platforms enable the control over the arrangement of the protruding rod-type molecular structures (e.g., molecular wires, switches, rotors, sensors with respect to the surface of the substrate.

Numeric computation and statistical data analysis on the Java platform

CERN Document Server

Chekanov, Sergei V

2016-01-01

Numerical computation, knowledge discovery and statistical data analysis integrated with powerful 2D and 3D graphics for visualization are the key topics of this book. The Python code examples powered by the Java platform can easily be transformed to other programming languages, such as Java, Groovy, Ruby and BeanShell. This book equips the reader with a computational platform which, unlike other statistical programs, is not limited by a single programming language. The author focuses on practical programming aspects and covers a broad range of topics, from basic introduction to the Python language on the Java platform (Jython), to descriptive statistics, symbolic calculations, neural networks, non-linear regression analysis and many other data-mining topics. He discusses how to find regularities in real-world data, how to classify data, and how to process data for knowledge discoveries. The code snippets are so short that they easily fit into single pages. Numeric Computation and Statistical Data Analysis ...

Demonstration Platform for near-zero energy buildings - small houses; Demonstrationsplattform foer naeranollenergibyggnader - smaahus

Energy Technology Data Exchange (ETDEWEB)

Ruud, Svein; Fahlen, Per; Axell, Monica; Kovacs, Peter; Ylmen, Peter; Staahl, Fredrik

2011-07-01

On behalf of the Swedish Energy Agency, SP has investigated and recommended how one could form a platform for demonstration of single family houses as nearly zero energy houses. SP suggests that TMF, the national trade and employers' association of the wood processing and furniture industry in Sweden, should administrate this platform. The reason being that members of TMF produce almost 80% of all single family houses in Sweden. TMF also has the capacity to launch a demonstration platform in a reasonable short time. SP has also compiled a set on technical criteria regarding properties related to energy use that should be met by demonstration projects within the platform. One presumption has been that a house that meets the criteria in the south of Sweden also should meet the criteria in the north of Sweden. The reason being to promote an industrialized and cost effective building process. Another ambition has been not to disfavor smaller single family houses. The main criteria are on very energy efficient building envelopes and very efficient building services systems. The criteria are therefore more detailed than the current Swedish building regulations

Virtual interconnection platform initiative scoping study

Energy Technology Data Exchange (ETDEWEB)

Liu, Yong [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Kou, Gefei [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Pan, Zuohong [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Liu, Yilu [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); King Jr., Thomas J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

2016-01-01

Due to security and liability concerns, the research community has limited access to realistic large-scale power grid models to test and validate new operation and control methodologies. It is also difficult for industry to evaluate the relative value of competing new tools without a common platform for comparison. This report proposes to develop a large-scale virtual power grid model that retains basic features and represents future trends of major U.S. electric interconnections. This model will include realistic power flow and dynamics information as well as a relevant geospatial distribution of assets. This model will be made widely available to the research community for various power system stability and control studies and can be used as a common platform for comparing the efficacies of various new technologies.

Cytobank: providing an analytics platform for community cytometry data analysis and collaboration.

Science.gov (United States)

Chen, Tiffany J; Kotecha, Nikesh

2014-01-01

Cytometry is used extensively in clinical and laboratory settings to diagnose and track cell subsets in blood and tissue. High-throughput, single-cell approaches leveraging cytometry are developed and applied in the computational and systems biology communities by researchers, who seek to improve the diagnosis of human diseases, map the structures of cell signaling networks, and identify new cell types. Data analysis and management present a bottleneck in the flow of knowledge from bench to clinic. Multi-parameter flow and mass cytometry enable identification of signaling profiles of patient cell samples. Currently, this process is manual, requiring hours of work to summarize multi-dimensional data and translate these data for input into other analysis programs. In addition, the increase in the number and size of collaborative cytometry studies as well as the computational complexity of analytical tools require the ability to assemble sufficient and appropriately configured computing capacity on demand. There is a critical need for platforms that can be used by both clinical and basic researchers who routinely rely on cytometry. Recent advances provide a unique opportunity to facilitate collaboration and analysis and management of cytometry data. Specifically, advances in cloud computing and virtualization are enabling efficient use of large computing resources for analysis and backup. An example is Cytobank, a platform that allows researchers to annotate, analyze, and share results along with the underlying single-cell data.

Geospatial Data Management Platform for Urban Groundwater

Science.gov (United States)

Gaitanaru, D.; Priceputu, A.; Gogu, C. R.

2012-04-01

Due to the large amount of civil work projects and research studies, large quantities of geo-data are produced for the urban environments. These data are usually redundant as well as they are spread in different institutions or private companies. Time consuming operations like data processing and information harmonisation represents the main reason to systematically avoid the re-use of data. The urban groundwater data shows the same complex situation. The underground structures (subway lines, deep foundations, underground parkings, and others), the urban facility networks (sewer systems, water supply networks, heating conduits, etc), the drainage systems, the surface water works and many others modify continuously. As consequence, their influence on groundwater changes systematically. However, these activities provide a large quantity of data, aquifers modelling and then behaviour prediction can be done using monitored quantitative and qualitative parameters. Due to the rapid evolution of technology in the past few years, transferring large amounts of information through internet has now become a feasible solution for sharing geoscience data. Furthermore, standard platform-independent means to do this have been developed (specific mark-up languages like: GML, GeoSciML, WaterML, GWML, CityML). They allow easily large geospatial databases updating and sharing through internet, even between different companies or between research centres that do not necessarily use the same database structures. For Bucharest City (Romania) an integrated platform for groundwater geospatial data management is developed under the framework of a national research project - "Sedimentary media modeling platform for groundwater management in urban areas" (SIMPA) financed by the National Authority for Scientific Research of Romania. The platform architecture is based on three components: a geospatial database, a desktop application (a complex set of hydrogeological and geological analysis

Pi-Sat: A Low Cost Small Satellite and Distributed Spacecraft Mission System Test Platform

Science.gov (United States)

Cudmore, Alan

2015-01-01

Current technology and budget trends indicate a shift in satellite architectures from large, expensive single satellite missions, to small, low cost distributed spacecraft missions. At the center of this shift is the SmallSatCubesat architecture. The primary goal of the Pi-Sat project is to create a low cost, and easy to use Distributed Spacecraft Mission (DSM) test bed to facilitate the research and development of next-generation DSM technologies and concepts. This test bed also serves as a realistic software development platform for Small Satellite and Cubesat architectures. The Pi-Sat is based on the popular $35 Raspberry Pi single board computer featuring a 700Mhz ARM processor, 512MB of RAM, a flash memory card, and a wealth of IO options. The Raspberry Pi runs the Linux operating system and can easily run Code 582s Core Flight System flight software architecture. The low cost and high availability of the Raspberry Pi make it an ideal platform for a Distributed Spacecraft Mission and Cubesat software development. The Pi-Sat models currently include a Pi-Sat 1U Cube, a Pi-Sat Wireless Node, and a Pi-Sat Cubesat processor card.The Pi-Sat project takes advantage of many popular trends in the Maker community including low cost electronics, 3d printing, and rapid prototyping in order to provide a realistic platform for flight software testing, training, and technology development. The Pi-Sat has also provided fantastic hands on training opportunities for NASA summer interns and Pathways students.

Parallel application of plasma equilibrium fitting based on inhomogeneous platforms

International Nuclear Information System (INIS)

Liao Min; Zhang Jinhua; Chen Liaoyuan; Li Yongge; Pan Wei; Pan Li

2008-01-01

An online analysis and online display platform EFIT, which is based on the equilibrium-fitting mode, is inducted in this paper. This application can realize large data transportation between inhomogeneous platforms by designing a communication mechanism using sockets. It spends approximately one minute to complete the equilibrium fitting reconstruction by using a finite state machine to describe the management node and several node computers of cluster system to fulfill the parallel computation, this satisfies the online display during the discharge interval. An effective communication model between inhomogeneous platforms is provided, which could transport the computing results from Linux platform to Windows platform for online analysis and display. (authors)

A unique aerial platform equipped for large area surveillance: a real-time tool for emergency management

International Nuclear Information System (INIS)

Frullani, Salvatore; Castelluccio, Donato M.; Cisbani, Evaristo; Colilli, Stefano; Fratoni, Rolando; Giuliani, Fausto; Mostarda, Angelo; Colangeli, Giorgio; De Otto, Gian L.; Marchiori, Carlo; Paoloni, Gianfranco

2008-01-01

Aerial platform equipped with a sampling line and real-time monitoring of sampled aerosol is presented. The system is composed by: a) A Sky Arrow 650 fixed wing aircraft with the front part of the fuselage properly adapted to house the detection and acquisition equipment; b) A compact air sampling line where the iso kinetic sampling is dynamically maintained, aerosol is collected on a filter positioned along the line and hosted on a rotating 4-filters disk; c) A detection subsystem: a small BGO scintillator and Geiger counter right behind the sampling filter, a HPGe detector allows radionuclide identification in the collected aerosol samples, a large NaI(Tl) crystal detects airborne and ground gamma radiation; d) Several environmental (temperature, pressure, aircraft/wind speed) sensors and a GPS receiver that support the full characterization of the sampling conditions and the temporal and geographical location of the acquired data; e) Acquisition and control system based on compact electronics and real time software that operate the sampling line actuators, guarantee the dynamical iso kinetic condition, and acquire the detectors and sensor data. With this system quantitative measurements can be available also during the plume phase of an accident, while other aerial platforms, without sampling capability, can only be used for qualitative assessments. Transmission of all data will be soon implemented in order to make all the data available in real-time to the Technical Centre for the Emergency Management. The use of an unmanned air-vehicle (UAV) is discussed as future option. (author)

Large-area aligned growth of single-crystalline organic nanowire arrays for high-performance photodetectors

International Nuclear Information System (INIS)

Wu Yiming; Zhang Xiujuan; Pan Huanhuan; Zhang Xiwei; Zhang Yuping; Zhang Xiaozhen; Jie Jiansheng

2013-01-01

Due to their extraordinary properties, single-crystalline organic nanowires (NWs) are important building blocks for future low-cost and efficient nano-optoelectronic devices. However, it remains a critical challenge to assemble organic NWs rationally in an orientation-, dimensionality- and location-controlled manner. Herein, we demonstrate a feasible method for aligned growth of single-crystalline copper phthalocyanine (CuPc) NW arrays with high density, large-area uniformity and perfect crossed alignment by using Au film as a template. The growth process was investigated in detail. The Au film was found to have a critical function in the aligned growth of NWs, but may only serve as the active site for NW nucleation because of the large surface energy, as well as direct the subsequent aligned growth. The as-prepared NWs were then transferred to construct single NW-based photoconductive devices, which demonstrated excellent photoresponse properties with robust stability and reproducibility; the device showed a high switching ratio of ∼180, a fast response speed of ∼100 ms and could stand continuous operation up to 2 h. Importantly, this strategy can be extended to other organic molecules for their synthesis of NW arrays, revealing great potential for use in the construction of large-scale high-performance functional nano-optoelectronic devices. (paper)

Semiempirical Quantum Chemical Calculations Accelerated on a Hybrid Multicore CPU-GPU Computing Platform.

Science.gov (United States)

Wu, Xin; Koslowski, Axel; Thiel, Walter

2012-07-10

In this work, we demonstrate that semiempirical quantum chemical calculations can be accelerated significantly by leveraging the graphics processing unit (GPU) as a coprocessor on a hybrid multicore CPU-GPU computing platform. Semiempirical calculations using the MNDO, AM1, PM3, OM1, OM2, and OM3 model Hamiltonians were systematically profiled for three types of test systems (fullerenes, water clusters, and solvated crambin) to identify the most time-consuming sections of the code. The corresponding routines were ported to the GPU and optimized employing both existing library functions and a GPU kernel that carries out a sequence of noniterative Jacobi transformations during pseudodiagonalization. The overall computation times for single-point energy calculations and geometry optimizations of large molecules were reduced by one order of magnitude for all methods, as compared to runs on a single CPU core.

PROBA-V Mission Exploitation Platform

Directory of Open Access Journals (Sweden)

Erwin Goor

2016-07-01

Full Text Available As an extension of the PROBA-Vegetation (PROBA-V user segment, the European Space Agency (ESA, de Vlaamse Instelling voor Technologisch Onderzoek (VITO, and partners TRASYS and Spacebel developed an operational Mission Exploitation Platform (MEP to drastically improve the exploitation of the PROBA-V Earth Observation (EO data archive, the archive from the historical SPOT-VEGETATION mission, and derived products by researchers, service providers, and thematic users. The analysis of the time series of data (petabyte range is addressed, as well as the large scale on-demand processing of the complete archive, including near real-time data. The platform consists of a private cloud environment, a Hadoop-based processing environment and a data manager. Several applications are released to the users, e.g., a full resolution viewing service, a time series viewer, pre-defined on-demand processing chains, and virtual machines with powerful tools and access to the data. After an initial release in January 2016 a research platform was deployed gradually, allowing users to design, debug, and test applications on the platform. From the PROBA-V MEP, access to, e.g., Sentinel-2 and Sentinel-3 data will be addressed as well.

Conducting Automated Test Assembly Using the Premium Solver Platform Version 7.0 with Microsoft Excel and the Large-Scale LP/QP Solver Engine Add-In

Science.gov (United States)

Cor, Ken; Alves, Cecilia; Gierl, Mark J.

2008-01-01

This review describes and evaluates a software add-in created by Frontline Systems, Inc., that can be used with Microsoft Excel 2007 to solve large, complex test assembly problems. The combination of Microsoft Excel 2007 with the Frontline Systems Premium Solver Platform is significant because Microsoft Excel is the most commonly used spreadsheet…

Contrasting platform thinking and product modularization

DEFF Research Database (Denmark)

Boer, Henrike Engele Elisabeth; Persson, Magnus

2015-01-01

Product modularization and platform thinking are both practices that seek to alleviate the negative impact of product customization and variety on internal operations by relying on economies of substitution. Through the use of a standardized pool of components and interfaces, these practices aim...... to create a broad spectrum of product choices. At first sight, product modularization and platform thinking are very similar. The difference between these practices can, however, be found in the manner in which they employ standardization. Where product modularization focuses on creating standardized...... variants. There is a general lack of research addressing the contingency factors that dictate the appropriateness of the use of product modularization and platform thinking in different contexts. To our knowledge, no large-scale empirical research has been reported in which the two concepts, contextual...

Max-Min SINR in Large-Scale Single-Cell MU-MIMO: Asymptotic Analysis and Low Complexity Transceivers

KAUST Repository

Sifaou, Houssem; Kammoun, Abla; Sanguinetti, Luca; Debbah, Merouane; Alouini, Mohamed-Slim

2016-01-01

This work focuses on the downlink and uplink of large-scale single-cell MU-MIMO systems in which the base station (BS) endowed with M antennas communicates with K single-antenna user equipments (UEs). Particularly, we aim at reducing the complexity

HardwareSoftware Co-design for Heterogeneous Multi-core Platforms The hArtes Toolchain

CERN Document Server

2012-01-01

This book describes the results and outcome of the FP6 project, known as hArtes, which focuses on the development of an integrated tool chain targeting a heterogeneous multi core platform comprising of a general purpose processor (ARM or powerPC), a DSP (the diopsis) and an FPGA. The tool chain takes existing source code and proposes transformations and mappings such that legacy code can easily be ported to a modern, multi-core platform. Benefits of the hArtes approach, described in this book, include: Uses a familiar programming paradigm: hArtes proposes a familiar programming paradigm which is compatible with the widely used programming practice, irrespective of the target platform. Enables users to view multiple cores as a single processor: the hArtes approach abstracts away the heterogeneity as well as the multi-core aspect of the underlying hardware so the developer can view the platform as consisting of a single, general purpose processor. Facilitates easy porting of existing applications: hArtes provid...

Dye molecules as single-photon sources and large optical nonlinearities on a chip

International Nuclear Information System (INIS)

Hwang, J; Hinds, E A

2011-01-01

We point out that individual organic dye molecules, deposited close to optical waveguides on a photonic chip, can act as single-photon sources. A thin silicon nitride strip waveguide is expected to collect 28% of the photons from a single dibenzoterrylene molecule. These molecules can also provide large, localized optical nonlinearities, which are enough to discriminate between one photon or two through a differential phase shift of 2 0 per photon. This new atom-photon interface may be used as a resource for processing quantum information.

A proficient and versatile online student-teacher collaboration platform for large classroom lectures

Directory of Open Access Journals (Sweden)

ABM Tariqul Islam

2017-11-01

Full Text Available Abstract The popularity of online collaboration on lecture content has been growing steadily over the last few decades because of its potential to enhance the overall learning experience. We propose a didactical approach of online collaboration where the students and the teachers can collaborate seamlessly on the lecture contents. The approach, which we call Multiscript (MS, offers two methods of online learning on one collaboration platform. In MS, we call one method the outside of class Multiscript (OMS and another, the inside of class Multiscript (IMS. OMS is a form of distance online learning where the students can collaborate on the lecture contents while being outside of class, whereas IMS allows online collaboration among the students and the teacher during the lecture. In OMS, the teacher can share the slides along with audio annotations for each lecture slides and/or a single recorded audio for the whole lecture. The students can access the slides and discuss (via text and audio chat with their fellow classmates about the slides and annotate them, post feedback about the slides and ask questions to the teacher directly via MS. In IMS, the students can create annotations for the slides and post feedback to the teacher about the slides. We design MS in such a way that it can be accessed by using just a web browser on any PC, tablet or mobile device.

Enumeration of antigen-specific CD8+ T lymphocytes by single-platform, HLA tetramer-based flow cytometry: a European multicenter evaluation.

Science.gov (United States)

Heijnen, Ingmar A F M; Barnett, David; Arroz, Maria J; Barry, Simon M; Bonneville, Marc; Brando, Bruno; D'hautcourt, Jean-Luc; Kern, Florian; Tötterman, Thomas H; Marijt, Erik W A; Bossy, David; Preijers, Frank W M B; Rothe, Gregor; Gratama, Jan W

2004-11-01

HLA class I peptide tetramers represent powerful diagnostic tools for detection and monitoring of antigen-specific CD8(+) T cells. The impetus for the current multicenter study is the critical need to standardize tetramer flow cytometry if it is to be implemented as a routine diagnostic assay. Hence, the European Working Group on Clinical Cell Analysis set out to develop and evaluate a single-platform tetramer-based method that used cytomegalovirus (CMV) as the antigenic model. Absolute numbers of CMV-specific CD8(+) T cells were obtained by combining the percentage of tetramer-binding cells with the absolute CD8(+) T-cell count. Six send-outs of stabilized blood from healthy individuals or CMV-carrying donors with CMV-specific CD8(+) T-cell counts of 3 to 10 cells/microl were distributed to 7 to 16 clinical sites. These sites were requested to enumerate CD8(+) T cells and, in the case of CMV-positive donors, CMV-specific subsets on three separate occasions using the standard method. Between-site coefficients of variation of less than 10% (absolute CD8(+) T-cell counts) and approximately 30% (percentage and absolute numbers of CMV-specific CD8(+) T cells) were achieved. Within-site coefficients of variation were approximately 5% (absolute CD8(+) T-cell counts), approximately 9% (percentage CMV-specific CD8(+) T cells), and approximately 17% (absolute CMV-specific CD8(+) T-cell counts). The degree of variation tended to correlate inversely with the proportion of CMV-specific CD8(+) T-cell subsets. The single-platform MHC tetramer-based method for antigen-specific CD8(+) T-cell counting has been evaluated by a European group of laboratories and can be considered a reproducible assay for routine enumeration of antigen-specific CD8(+) T cells. (c) 2004 Wiley-Liss, Inc.

Lupus-related single nucleotide polymorphisms and risk of diffuse large B-cell lymphoma

NARCIS (Netherlands)

Bernatsky, Sasha; Velásquez García, Héctor A; Spinelli, John; Gaffney, Patrick; Smedby, Karin E; Ramsey-Goldman, Rosalind; Wang, Sophia S.; Adami, Hans-Olov; Albanes, Demetrius; Angelucci, Emanuele; Ansell, Stephen M.; Asmann, Yan W.; Becker, Nikolaus; Benavente, Yolanda; Berndt, Sonja I.; Bertrand, Kimberly A.; Birmann, Brenda M.; Boeing, Heiner; Boffetta, Paolo; Bracci, Paige M.; Brennan, Paul; Brooks-Wilson, Angela R.; Cerhan, James R.; Chanock, Stephen J.; Clavel, Jacqueline; Conde, Lucia; Cotenbader, Karen H; Cox, David G; Cozen, Wendy; Crouch, Simon; De Roos, Anneclaire J.; De Sanjose, Silvia; Di Lollo, Simonetta; Diver, W. Ryan; Dogan, Ahmet; Foretova, Lenka; Ghesquières, Hervé; Giles, Graham G.; Glimelius, Bengt; Habermann, Thomas M.; Haioun, Corinne; Hartge, Patricia; Hjalgrim, Henrik; Holford, Theodore R.; Holly, Elizabeth A.; Jackson, Rebecca D.; Kaaks, Rudolph; Kane, Eleanor; Kelly, Rachel S.; Klein, Robert J.; Kraft, Peter; Kricker, Anne; Lan, Qing; Lawrence, Charles; Liebow, Mark; Lightfoot, Tracy; Link, Brian K.; Maynadie, Marc; McKay, James; Melbye, Mads; Molina, Thierry Jo; Monnereau, Alain; Morton, Lindsay M.; Nieters, Alexandra; North, Kari E.; Novak, Anne J.; Offit, Kenneth; Purdue, Mark P.; Rais, Marco; Riby, Jacques; Roman, Eve; Rothman, Nathaniel; Salles, Gilles; Severi, Gianluca; Severson, Richard K.; Skibola, Christine F.; Slager, Susan L.; Smith, Alex; Smith, Martyn T.; Southey, Melissa C.; Staines, Anthony; Teras, Lauren R.; Thompson, Carrie A.; Tilly, Hervé; Tinker, Lesley F.; Tjonneland, Anne; Turner, Jenny; Vajdic, Claire M.; Vermeulen, Roel C H; Vijai, Joseph; Vineis, Paolo; Virtamo, Jarmo; Wang, Zhaoming; Weinstein, Stephanie; Witzig, Thomas E.; Zelenetz, Andrew; Zeleniuch-Jacquotte, Anne; Zhang, Yawei; Zheng, Tongzhang; Zucca, Mariagrazia; Clarke, Ann E

2017-01-01

Objective: Determinants of the increased risk of diffuse large B-cell lymphoma (DLBCL) in SLE are unclear. Using data from a recent lymphoma genome-wide association study (GWAS), we assessed whether certain lupus-related single nucleotide polymorphisms (SNPs) were also associated with DLBCL.

Industry Platforms and New Industrial Policy in Russia

Directory of Open Access Journals (Sweden)

Svetlana V. Orekhova

2017-12-01

Full Text Available The article aims at clarifying Russian industrial policy as a result of business models changing and market reconstruction. The research bass on the hypothesis that the choice of the industrial policy administrative measures depends on the object of management. It is important to link government regulation of markets with corporate strategies. We reveal that modern economic systems based on using of electronic technologies, big data, and innovative activity. Technological platforms as the single organizational and economic mechanisms affect economic systems very much. We study the content and main characteristics of a business model, is called the "technological platform". We also identify the main required changes in industrial policy. We analyze the matching between scientific and technological scenarios of the Russian economic development and the technological platform. There are two areas of the new industrial policy. They are: a multi-sectoral approach to regulating and improving the quality of the national institutional environment. The industrial and cluster management approaches are inefficient in modern conditions. There is a need for each platform of its technological development scenario. We also clarify the role of the state in the functioning of technological platforms.

Methods for the preparation of large quantities of complex single-stranded oligonucleotide libraries.

Science.gov (United States)

Murgha, Yusuf E; Rouillard, Jean-Marie; Gulari, Erdogan

2014-01-01

Custom-defined oligonucleotide collections have a broad range of applications in fields of synthetic biology, targeted sequencing, and cytogenetics. Also, they are used to encode information for technologies like RNA interference, protein engineering and DNA-encoded libraries. High-throughput parallel DNA synthesis technologies developed for the manufacture of DNA microarrays can produce libraries of large numbers of different oligonucleotides, but in very limited amounts. Here, we compare three approaches to prepare large quantities of single-stranded oligonucleotide libraries derived from microarray synthesized collections. The first approach, alkaline melting of double-stranded PCR amplified libraries with a biotinylated strand captured on streptavidin coated magnetic beads results in little or no non-biotinylated ssDNA. The second method wherein the phosphorylated strand of PCR amplified libraries is nucleolyticaly hydrolyzed is recommended when small amounts of libraries are needed. The third method combining in vitro transcription of PCR amplified libraries to reverse transcription of the RNA product into single-stranded cDNA is our recommended method to produce large amounts of oligonucleotide libraries. Finally, we propose a method to remove any primer binding sequences introduced during library amplification.

GRAMI: Frequent subgraph and pattern mining in a single large graph

KAUST Repository

Elseidy, M.

2014-01-01

Mining frequent subgraphs is an important operation on graphs; it is defined as finding all subgraphs that appear frequently in a database according to a given frequency threshold. Most existing work assumes a database of many small graphs, but modern applications, such as social networks, citation graphs, or proteinprotein interactions in bioinformatics, are modeled as a single large graph. In this paper we present GRAMI, a novel framework for frequent subgraph mining in a single large graph. GRAMI undertakes a novel approach that only finds the minimal set of instances to satisfy the frequency threshold and avoids the costly enumeration of all instances required by previous approaches. We accompany our approach with a heuristic and optimizations that significantly improve performance. Additionally, we present an extension of GRAMI that mines frequent patterns. Compared to subgraphs, patterns offer a more powerful version of matching that captures transitive interactions between graph nodes (like friend of a friend) which are very common in modern applications. Finally, we present CGRAMI, a version supporting structural and semantic constraints, and AGRAMI, an approximate version producing results with no false positives. Our experiments on real data demonstrate that our framework is up to 2 orders of magnitude faster and discovers more interesting patterns than existing approaches. 2014 VLDB Endowment.

Edge field emission of large-area single layer graphene

Energy Technology Data Exchange (ETDEWEB)

Kleshch, Victor I., E-mail: klesch@polly.phys.msu.ru [Department of Physics, M.V. Lomonosov Moscow State University, Moscow 119991 (Russian Federation); Bandurin, Denis A. [Department of Physics, M.V. Lomonosov Moscow State University, Moscow 119991 (Russian Federation); Orekhov, Anton S. [Department of Physics, M.V. Lomonosov Moscow State University, Moscow 119991 (Russian Federation); A.V. Shubnikov Institute of Crystallography, RAS, Moscow 119333 (Russian Federation); Purcell, Stephen T. [ILM, Université Claude Bernard Lyon 1 et CNRS, UMR 5586, 69622 Villeurbanne (France); Obraztsov, Alexander N. [Department of Physics, M.V. Lomonosov Moscow State University, Moscow 119991 (Russian Federation); Department of Physics and Mathematics, University of Eastern Finland, Joensuu 80101 (Finland)

2015-12-01

Graphical abstract: - Highlights: • Stable field emission was observed from the edge of large-area graphene on quartz. • A strong hysteresis in current–voltage characteristics was observed. • The hysteresis was explained by mechanical peeling of graphene edge from substrate. • Reversible peeling of graphene edge may be used in microelectromechanical systems. - Abstract: Field electron emission from the edges of large-area (∼1 cm × 1 cm) graphene films deposited onto quartz wafers was studied. The graphene was previously grown by chemical vapour deposition on copper. An extreme enhancement of electrostatic field at the edge of the films with macroscopically large lateral dimensions and with single atom thickness was achieved. This resulted in the creation of a blade type electron emitter, providing stable field emission at low-voltage with linear current density up to 0.5 mA/cm. A strong hysteresis in current–voltage characteristics and a step-like increase of the emission current during voltage ramp up were observed. These effects were explained by the local mechanical peeling of the graphene edge from the quartz substrate by the ponderomotive force during the field emission process. Specific field emission phenomena exhibited in the experimental study are explained by a unique combination of structural, electronic and mechanical properties of graphene. Various potential applications ranging from linear electron beam sources to microelectromechanical systems are discussed.

Application of the CometChip platform to assess DNA damage in field-collected blood samples from turtles.

Science.gov (United States)

Sykora, Peter; Chiari, Ylenia; Heaton, Andrew; Moreno, Nickolas; Glaberman, Scott; Sobol, Robert W

2018-05-01

DNA damage has been linked to genomic instability and the progressive breakdown of cellular and organismal homeostasis, leading to the onset of disease and reduced longevity. Insults to DNA from endogenous sources include base deamination, base hydrolysis, base alkylation, and metabolism-induced oxidative damage that can lead to single-strand and double-strand DNA breaks. Alternatively, exposure to environmental pollutants, radiation or ultra-violet light, can also contribute to exogenously derived DNA damage. We previously validated a novel, high through-put approach to measure levels of DNA damage in cultured mammalian cells. This new CometChip Platform builds on the classical single cell gel electrophoresis or comet methodology used extensively in environmental toxicology and molecular biology. We asked whether the CometChip Platform could be used to measure DNA damage in samples derived from environmental field studies. To this end, we determined that nucleated erythrocytes from multiple species of turtle could be successfully evaluated in the CometChip Platform to quantify levels of DNA damage. In total, we compared levels of DNA damage in 40 animals from two species: the box turtle (Terrapene carolina) and the red-eared slider (Trachemys scripta elegans). Endogenous levels of DNA damage were identical between the two species, yet we did discover some sex-linked differences and changes in DNA damage accumulation. Based on these results, we confirm that the CometChip Platform allows for the measurement of DNA damage in a large number of samples quickly and accurately, and is particularly adaptable to environmental studies using field-collected samples. Environ. Mol. Mutagen. 59:322-333, 2018. © 2018 Wiley Periodicals, Inc. © 2018 Wiley Periodicals, Inc.

Effect of platform connection and abutment material on stress distribution in single anterior implant-supported restorations: a nonlinear 3-dimensional finite element analysis.

Science.gov (United States)

Carvalho, Marco Aurélio; Sotto-Maior, Bruno Salles; Del Bel Cury, Altair Antoninha; Pessanha Henriques, Guilherme Elias

2014-11-01

Although various abutment connections and materials have recently been introduced, insufficient data exist regarding the effect of stress distribution on their mechanical performance. The purpose of this study was to investigate the effect of different abutment materials and platform connections on stress distribution in single anterior implant-supported restorations with the finite element method. Nine experimental groups were modeled from the combination of 3 platform connections (external hexagon, internal hexagon, and Morse tapered) and 3 abutment materials (titanium, zirconia, and hybrid) as follows: external hexagon-titanium, external hexagon-zirconia, external hexagon-hybrid, internal hexagon-titanium, internal hexagon-zirconia, internal hexagon-hybrid, Morse tapered-titanium, Morse tapered-zirconia, and Morse tapered-hybrid. Finite element models consisted of a 4×13-mm implant, anatomic abutment, and lithium disilicate central incisor crown cemented over the abutment. The 49 N occlusal loading was applied in 6 steps to simulate the incisal guidance. Equivalent von Mises stress (σvM) was used for both the qualitative and quantitative evaluation of the implant and abutment in all the groups and the maximum (σmax) and minimum (σmin) principal stresses for the numerical comparison of the zirconia parts. The highest abutment σvM occurred in the Morse-tapered groups and the lowest in the external hexagon-hybrid, internal hexagon-titanium, and internal hexagon-hybrid groups. The σmax and σmin values were lower in the hybrid groups than in the zirconia groups. The stress distribution concentrated in the abutment-implant interface in all the groups, regardless of the platform connection or abutment material. The platform connection influenced the stress on abutments more than the abutment material. The stress values for implants were similar among different platform connections, but greater stress concentrations were observed in internal connections

Free Global Dsm Assessment on Large Scale Areas Exploiting the Potentialities of the Innovative Google Earth Engine Platform

Science.gov (United States)

Nascetti, A.; Di Rita, M.; Ravanelli, R.; Amicuzi, M.; Esposito, S.; Crespi, M.

2017-05-01

The high-performance cloud-computing platform Google Earth Engine has been developed for global-scale analysis based on the Earth observation data. In particular, in this work, the geometric accuracy of the two most used nearly-global free DSMs (SRTM and ASTER) has been evaluated on the territories of four American States (Colorado, Michigan, Nevada, Utah) and one Italian Region (Trentino Alto- Adige, Northern Italy) exploiting the potentiality of this platform. These are large areas characterized by different terrain morphology, land covers and slopes. The assessment has been performed using two different reference DSMs: the USGS National Elevation Dataset (NED) and a LiDAR acquisition. The DSMs accuracy has been evaluated through computation of standard statistic parameters, both at global scale (considering the whole State/Region) and in function of the terrain morphology using several slope classes. The geometric accuracy in terms of Standard deviation and NMAD, for SRTM range from 2-3 meters in the first slope class to about 45 meters in the last one, whereas for ASTER, the values range from 5-6 to 30 meters. In general, the performed analysis shows a better accuracy for the SRTM in the flat areas whereas the ASTER GDEM is more reliable in the steep areas, where the slopes increase. These preliminary results highlight the GEE potentialities to perform DSM assessment on a global scale.

FREE GLOBAL DSM ASSESSMENT ON LARGE SCALE AREAS EXPLOITING THE POTENTIALITIES OF THE INNOVATIVE GOOGLE EARTH ENGINE PLATFORM

Directory of Open Access Journals (Sweden)

A. Nascetti

2017-05-01

Full Text Available The high-performance cloud-computing platform Google Earth Engine has been developed for global-scale analysis based on the Earth observation data. In particular, in this work, the geometric accuracy of the two most used nearly-global free DSMs (SRTM and ASTER has been evaluated on the territories of four American States (Colorado, Michigan, Nevada, Utah and one Italian Region (Trentino Alto- Adige, Northern Italy exploiting the potentiality of this platform. These are large areas characterized by different terrain morphology, land covers and slopes. The assessment has been performed using two different reference DSMs: the USGS National Elevation Dataset (NED and a LiDAR acquisition. The DSMs accuracy has been evaluated through computation of standard statistic parameters, both at global scale (considering the whole State/Region and in function of the terrain morphology using several slope classes. The geometric accuracy in terms of Standard deviation and NMAD, for SRTM range from 2-3 meters in the first slope class to about 45 meters in the last one, whereas for ASTER, the values range from 5-6 to 30 meters. In general, the performed analysis shows a better accuracy for the SRTM in the flat areas whereas the ASTER GDEM is more reliable in the steep areas, where the slopes increase. These preliminary results highlight the GEE potentialities to perform DSM assessment on a global scale.

Single-mode regime in large-mode-area rare-earth-doped rod-type PCFs

DEFF Research Database (Denmark)

Poli, F.; Cucinotta, A.; Passaro, D.

2009-01-01

In this paper, large-mode-area, double-cladding, rare-earth-doped photonic crystal fibers are investigated in order to understand how the refractive index distribution and the mode competition given by the amplification can assure single-mode propagation. Fibers with different core diameters, i...

University technology platform of anticipatory learning

Directory of Open Access Journals (Sweden)

Leonid Davidovich Gitelman

2016-03-01

Full Text Available The innovative development sets large-scale and challenging tasks, which need to be addressed in the lack-of-knowledge conditions and require the coordination and integration of numerous expert structures, which are scattered around the world and have different status and competencies. One of the mechanisms of integrating the partners’ intellectual and financial resources is provided by the technology platforms. The article discusses the nature and functions of technology platforms and analyzes the experience of their application in different countries with a special emphasis on universities. The article gives an overview of the various interpretations of technology platform concepts. It also describes the development and implementation of the technological platform at the Ural Federal University (research and education centre ‘ENGEC’, which was targeted at organizing anticipatory learning in the sphere of energy engineering and high-tech industries; its mechanism and role in improving different university activities and processes are shown. This platform is based on the original methodology ‘Integrated System of Consulting, Training, and Transformation’ (ISCT, which includes authentic methods and technologies, which are used in the educational process. A significant advantage of this methodology is that it can be applied in university education as well as in corporate training integrated with innovative activities.

Analyzing composability of applications on MPSoC platforms

NARCIS (Netherlands)

Kumar, A.; Mesman, B.; Theelen, B.D.; Corporaal, H.; Yajun, H.

2008-01-01

Modern day applications require use of multi-processor systems for reasons of erformance, scalability and power efficiency. As more and more applications are integrated in a single system, mapping and analyzing them on a multi-processor platform becomes a multidimensional problem. Each possible set

A QFD-based optimization method for a scalable product platform

Science.gov (United States)

Luo, Xinggang; Tang, Jiafu; Kwong, C. K.

2010-02-01

In order to incorporate the customer into the early phase of the product development cycle and to better satisfy customers' requirements, this article adopts quality function deployment (QFD) for optimal design of a scalable product platform. A five-step QFD-based method is proposed to determine the optimal values for platform engineering characteristics (ECs) and non-platform ECs of the products within a product family. First of all, the houses of quality (HoQs) for all product variants are developed and a QFD-based optimization approach is used to determine the optimal ECs for each product variant. Sensitivity analysis is performed for each EC with respect to overall customer satisfaction (OCS). Based on the obtained sensitivity indices of ECs, a mathematical model is established to simultaneously optimize the values of the platform and the non-platform ECs. Finally, by comparing and analysing the optimal solutions with different number of platform ECs, the ECs with which the worst OCS loss can be avoided are selected as platform ECs. An illustrative example is used to demonstrate the feasibility of this method. A comparison between the proposed method and a two-step approach is conducted on the example. The comparison shows that, as a kind of single-stage approach, the proposed method yields better average degree of customer satisfaction due to the simultaneous optimization of platform and non-platform ECs.

Multimodal Microchannel and Nanowell-Based Microfluidic Platforms for Bioimaging

Energy Technology Data Exchange (ETDEWEB)

Geng, Tao; Smallwood, Chuck R.; Zhu, Ying; Bredeweg, Erin L.; Baker, Scott E.; Evans, James E.; Kelly, Ryan T.

2017-03-30

Modern live-cell imaging approaches permit real-time visualization of biological processes. However, limitations for unicellular organism trapping, culturing and long-term imaging can preclude complete understanding of how such microorganisms respond to perturbations in their local environment or linking single-cell variability to whole population dynamics. We have developed microfluidic platforms to overcome prior technical bottlenecks to allow both chemostat and compartmentalized cellular growth conditions using the same device. Additionally, a nanowell-based platform enables a high throughput approach to scale up compartmentalized imaging optimized within the microfluidic device. These channel and nanowell platforms are complementary, and both provide fine control over the local environment as well as the ability to add/replace media components at any experimental time point.

Large curvature and background scale independence in single-metric approximations to asymptotic safety

Energy Technology Data Exchange (ETDEWEB)

Morris, Tim R. [STAG Research Centre & Department of Physics and Astronomy, University of Southampton,Highfield, Southampton, SO17 1BJ (United Kingdom)

2016-11-25

In single-metric approximations to the exact renormalization group (RG) for quantum gravity, it has been not been clear how to treat the large curvature domain beyond the point where the effective cutoff scale k is less than the lowest eigenvalue of the appropriate modified Laplacian. We explain why this puzzle arises from background dependence, resulting in Wilsonian RG concepts being inapplicable. We show that when properly formulated over an ensemble of backgrounds, the Wilsonian RG can be restored. This in turn implies that solutions should be smooth and well defined no matter how large the curvature is taken. Even for the standard single-metric type approximation schemes, this construction can be rigorously derived by imposing a modified Ward identity (mWI) corresponding to rescaling the background metric by a constant factor. However compatibility in this approximation requires the space-time dimension to be six. Solving the mWI and flow equation simultaneously, new variables are then derived that are independent of overall background scale.

Design of a Large Single-Aperture Dipole Magnet for HL-LHC Upgrade

CERN Document Server

Qingjin, Xu; Iio, Masami; Ogitsu, Toru; Sasaki, Kenichi; Yamamoto, Akira; Todesco, Ezio

2013-01-01

An upgrade of the low-beta insertion system for the ATLAS and Compact Muon Solenoid experiments is proposed in the high luminosity Large Hadron Collider upgrade project. It includes final beam focusing quadrupoles, beam separation and recombination dipoles, and larger aperture matching section quadrupoles. KEK is in charge of the conceptual design of the large aperture separation dipole D1. The latest design parameters are a main field of ~ 5 T at 1.9 K with Nb-Ti superconducting technology, a coil aperture of 160 mm, and a cos-theta one-layer coil with Large Hadron Collider dipole cable. Because the new D1 is expected to be operated in a very high radiation environment, radiation resistance and a cooling scheme are being carefully considered. The collaring-yoke structure is adopted to provide the mechanical support for the single-layer Nb-Ti coil. We summarize the design study of this magnet, including i) the very large iron saturation effect on field quality due to the large aperture and limited size of the...

Distributed hash table theory, platforms and applications

CERN Document Server

Zhang, Hao; Xie, Haiyong; Yu, Nenghai

2013-01-01

This SpringerBrief summarizes the development of Distributed Hash Table in both academic and industrial fields. It covers the main theory, platforms and applications of this key part in distributed systems and applications, especially in large-scale distributed environments. The authors teach the principles of several popular DHT platforms that can solve practical problems such as load balance, multiple replicas, consistency and latency. They also propose DHT-based applications including multicast, anycast, distributed file systems, search, storage, content delivery network, file sharing and c

Evaluation of Smartphone Inertial Sensor Performance for Cross-Platform Mobile Applications

Science.gov (United States)

Kos, Anton; Tomažič, Sašo; Umek, Anton

2016-01-01

Smartphone sensors are being increasingly used in mobile applications. The performance of sensors varies considerably among different smartphone models and the development of a cross-platform mobile application might be a very complex and demanding task. A publicly accessible resource containing real-life-situation smartphone sensor parameters could be of great help for cross-platform developers. To address this issue we have designed and implemented a pilot participatory sensing application for measuring, gathering, and analyzing smartphone sensor parameters. We start with smartphone accelerometer and gyroscope bias and noise parameters. The application database presently includes sensor parameters of more than 60 different smartphone models of different platforms. It is a modest, but important start, offering information on several statistical parameters of the measured smartphone sensors and insights into their performance. The next step, a large-scale cloud-based version of the application, is already planned. The large database of smartphone sensor parameters may prove particularly useful for cross-platform developers. It may also be interesting for individual participants who would be able to check-up and compare their smartphone sensors against a large number of similar or identical models. PMID:27049391

Evaluation of Smartphone Inertial Sensor Performance for Cross-Platform Mobile Applications

Directory of Open Access Journals (Sweden)

Anton Kos

2016-04-01

Full Text Available Smartphone sensors are being increasingly used in mobile applications. The performance of sensors varies considerably among different smartphone models and the development of a cross-platform mobile application might be a very complex and demanding task. A publicly accessible resource containing real-life-situation smartphone sensor parameters could be of great help for cross-platform developers. To address this issue we have designed and implemented a pilot participatory sensing application for measuring, gathering, and analyzing smartphone sensor parameters. We start with smartphone accelerometer and gyroscope bias and noise parameters. The application database presently includes sensor parameters of more than 60 different smartphone models of different platforms. It is a modest, but important start, offering information on several statistical parameters of the measured smartphone sensors and insights into their performance. The next step, a large-scale cloud-based version of the application, is already planned. The large database of smartphone sensor parameters may prove particularly useful for cross-platform developers. It may also be interesting for individual participants who would be able to check-up and compare their smartphone sensors against a large number of similar or identical models.

Analysis and experiments of a novel and compact 3-DOF precision positioning platform

International Nuclear Information System (INIS)

Huang, Hu; Zhao, Hongwei; Fan, Zunqiang; Zhang, Hui; Ma, Zhichao; Yang, Zhaojun

2013-01-01

A novel 3-DOF precision positioning platform with dimensions of 48 mm X 50 mm X 35 mm was designed by integrating piezo actuators and flexure hinges. The platform has a compact structure but it can do high precision positioning in three axes. The dynamic model of the platform in a single direction was established. Stiffness of the flexure hinges and modal characteristics of the flexure hinge mechanism were analyzed by the finite element method. Output displacements of the platform along three axes were forecasted via stiffness analysis. Output performance of the platform in x and y axes with open-loop control as well as the z-axis with closed-loop control was tested and discussed. The preliminary application of the platform in the field of nanoindentation indicates that the designed platform works well during nanoindentation tests, and the closed-loop control ensures the linear displacement output. With suitable control, the platform has the potential to realize different positioning functions under various working conditions.

Payment Platform

DEFF Research Database (Denmark)

Hjelholt, Morten; Damsgaard, Jan

2012-01-01

thoroughly and substitute current payment standards in the decades to come. This paper portrays how digital payment platforms evolve in socio-technical niches and how various technological platforms aim for institutional attention in their attempt to challenge earlier platforms and standards. The paper...... applies a co-evolutionary multilevel perspective to model the interplay and processes between technology and society wherein digital payment platforms potentially will substitute other payment platforms just like the credit card negated the check. On this basis this paper formulate a multilevel conceptual...

Comparative Performance in Single-Port Versus Multiport Minimally Invasive Surgery, and Small Versus Large Operative Working Spaces: A Preclinical Randomized Crossover Trial.

Science.gov (United States)

Marcus, Hani J; Seneci, Carlo A; Hughes-Hallett, Archie; Cundy, Thomas P; Nandi, Dipankar; Yang, Guang-Zhong; Darzi, Ara

2016-04-01

Surgical approaches such as transanal endoscopic microsurgery, which utilize small operative working spaces, and are necessarily single-port, are particularly demanding with standard instruments and have not been widely adopted. The aim of this study was to compare simultaneously surgical performance in single-port versus multiport approaches, and small versus large working spaces. Ten novice, 4 intermediate, and 1 expert surgeons were recruited from a university hospital. A preclinical randomized crossover study design was implemented, comparing performance under the following conditions: (1) multiport approach and large working space, (2) multiport approach and intermediate working space, (3) single-port approach and large working space, (4) single-port approach and intermediate working space, and (5) single-port approach and small working space. In each case, participants performed a peg transfer and pattern cutting tasks, and each task repetition was scored. Intermediate and expert surgeons performed significantly better than novices in all conditions (P Performance in single-port surgery was significantly worse than multiport surgery (P performance in the intermediate versus large working space. In single-port surgery, there was a converse trend; performances in the intermediate and small working spaces were significantly better than in the large working space. Single-port approaches were significantly more technically challenging than multiport approaches, possibly reflecting loss of instrument triangulation. Surprisingly, in single-port approaches, in which triangulation was no longer a factor, performance in large working spaces was worse than in intermediate and small working spaces. © The Author(s) 2015.

Techniques for extracting single-trial activity patterns from large-scale neural recordings

Science.gov (United States)

Churchland, Mark M; Yu, Byron M; Sahani, Maneesh; Shenoy, Krishna V

2008-01-01

Summary Large, chronically-implanted arrays of microelectrodes are an increasingly common tool for recording from primate cortex, and can provide extracellular recordings from many (order of 100) neurons. While the desire for cortically-based motor prostheses has helped drive their development, such arrays also offer great potential to advance basic neuroscience research. Here we discuss the utility of array recording for the study of neural dynamics. Neural activity often has dynamics beyond that driven directly by the stimulus. While governed by those dynamics, neural responses may nevertheless unfold differently for nominally identical trials, rendering many traditional analysis methods ineffective. We review recent studies – some employing simultaneous recording, some not – indicating that such variability is indeed present both during movement generation, and during the preceding premotor computations. In such cases, large-scale simultaneous recordings have the potential to provide an unprecedented view of neural dynamics at the level of single trials. However, this enterprise will depend not only on techniques for simultaneous recording, but also on the use and further development of analysis techniques that can appropriately reduce the dimensionality of the data, and allow visualization of single-trial neural behavior. PMID:18093826

Design of an omnidirectional single-point photodetector for large-scale spatial coordinate measurement

Science.gov (United States)

Xie, Hongbo; Mao, Chensheng; Ren, Yongjie; Zhu, Jigui; Wang, Chao; Yang, Lei

2017-10-01

In high precision and large-scale coordinate measurement, one commonly used approach to determine the coordinate of a target point is utilizing the spatial trigonometric relationships between multiple laser transmitter stations and the target point. A light receiving device at the target point is the key element in large-scale coordinate measurement systems. To ensure high-resolution and highly sensitive spatial coordinate measurement, a high-performance and miniaturized omnidirectional single-point photodetector (OSPD) is greatly desired. We report one design of OSPD using an aspheric lens, which achieves an enhanced reception angle of -5 deg to 45 deg in vertical and 360 deg in horizontal. As the heart of our OSPD, the aspheric lens is designed in a geometric model and optimized by LightTools Software, which enables the reflection of a wide-angle incident light beam into the single-point photodiode. The performance of home-made OSPD is characterized with working distances from 1 to 13 m and further analyzed utilizing developed a geometric model. The experimental and analytic results verify that our device is highly suitable for large-scale coordinate metrology. The developed device also holds great potential in various applications such as omnidirectional vision sensor, indoor global positioning system, and optical wireless communication systems.

Microchip screening platform for single cell assessment of NK cell cytotoxicity

Directory of Open Access Journals (Sweden)

Karolin eGuldevall

2016-04-01

Full Text Available Here we report a screening platform for assessment of the cytotoxic potential of individual natural killer (NK cells within larger populations. Human primary NK cells were distributed across a silicon-glass microchip containing 32 400 individual microwells loaded with target cells. Through fluorescence screening and automated image analysis the numbers of NK and live or dead target cells in each well could be assessed at different time points after initial mixing. Cytotoxicity was also studied by time-lapse live-cell imaging in microwells quantifying the killing potential of individual NK cells. Although most resting NK cells (≈75% were non-cytotoxic against the leukemia cell line K562, some NK cells were able to kill several (≥3 target cells within the 12 hours long experiment. In addition, the screening approach was adapted to increase the chance to find and evaluate serial killing NK cells. Even if the cytotoxic potential varied between donors it was evident that a small fraction of highly cytotoxic NK cells were responsible for a substantial portion of the killing. We demonstrate multiple assays where our platform can be used to enumerate and characterize cytotoxic cells, such as NK or T cells. This approach could find use in clinical applications, e.g. in the selection of donors for stem cell transplantation or generation of highly specific and cytotoxic cells for adoptive immunotherapy.

Microchip Screening Platform for Single Cell Assessment of NK Cell Cytotoxicity

Science.gov (United States)

Guldevall, Karolin; Brandt, Ludwig; Forslund, Elin; Olofsson, Karl; Frisk, Thomas W.; Olofsson, Per E.; Gustafsson, Karin; Manneberg, Otto; Vanherberghen, Bruno; Brismar, Hjalmar; Kärre, Klas; Uhlin, Michael; Önfelt, Björn

2016-01-01

Here, we report a screening platform for assessment of the cytotoxic potential of individual natural killer (NK) cells within larger populations. Human primary NK cells were distributed across a silicon–glass microchip containing 32,400 individual microwells loaded with target cells. Through fluorescence screening and automated image analysis, the numbers of NK and live or dead target cells in each well could be assessed at different time points after initial mixing. Cytotoxicity was also studied by time-lapse live-cell imaging in microwells quantifying the killing potential of individual NK cells. Although most resting NK cells (≈75%) were non-cytotoxic against the leukemia cell line K562, some NK cells were able to kill several (≥3) target cells within the 12-h long experiment. In addition, the screening approach was adapted to increase the chance to find and evaluate serial killing NK cells. Even if the cytotoxic potential varied between donors, it was evident that a small fraction of highly cytotoxic NK cells were responsible for a substantial portion of the killing. We demonstrate multiple assays where our platform can be used to enumerate and characterize cytotoxic cells, such as NK or T cells. This approach could find use in clinical applications, e.g., in the selection of donors for stem cell transplantation or generation of highly specific and cytotoxic cells for adoptive immunotherapy. PMID:27092139

Cross-platform analysis of cancer microarray data improves gene expression based classification of phenotypes

Directory of Open Access Journals (Sweden)

Eils Roland

2005-11-01

Full Text Available Abstract Background The extensive use of DNA microarray technology in the characterization of the cell transcriptome is leading to an ever increasing amount of microarray data from cancer studies. Although similar questions for the same type of cancer are addressed in these different studies, a comparative analysis of their results is hampered by the use of heterogeneous microarray platforms and analysis methods. Results In contrast to a meta-analysis approach where results of different studies are combined on an interpretative level, we investigate here how to directly integrate raw microarray data from different studies for the purpose of supervised classification analysis. We use median rank scores and quantile discretization to derive numerically comparable measures of gene expression from different platforms. These transformed data are then used for training of classifiers based on support vector machines. We apply this approach to six publicly available cancer microarray gene expression data sets, which consist of three pairs of studies, each examining the same type of cancer, i.e. breast cancer, prostate cancer or acute myeloid leukemia. For each pair, one study was performed by means of cDNA microarrays and the other by means of oligonucleotide microarrays. In each pair, high classification accuracies (> 85% were achieved with training and testing on data instances randomly chosen from both data sets in a cross-validation analysis. To exemplify the potential of this cross-platform classification analysis, we use two leukemia microarray data sets to show that important genes with regard to the biology of leukemia are selected in an integrated analysis, which are missed in either single-set analysis. Conclusion Cross-platform classification of multiple cancer microarray data sets yields discriminative gene expression signatures that are found and validated on a large number of microarray samples, generated by different laboratories and

OTEC Cold Water Pipe-Platform Subsystem Dynamic Interaction Validation

Energy Technology Data Exchange (ETDEWEB)

Varley, Robert [Lockheed Martin Corporation, Manassas, VA (United States); Halkyard, John [John Halkyard and Associates, Houston, TX (United States); Johnson, Peter [BMT Scientific Marine Services, Inc., Houston, TX (United States); Shi, Shan [Houston Offshore Engineering, Houston, TX (United States); Marinho, Thiago [Federal Univ. of Rio de Janeiro (Brazil). LabOceano

2014-05-09

A commercial floating 100-megawatt (MW) ocean thermal energy conversion (OTEC) power plant will require a cold water pipe (CWP) with a diameter of 10-meter (m) and length of up to 1,000 m. The mass of the cold water pipe, including entrained water, can exceed the mass of the platform supporting it. The offshore industry uses software-modeling tools to develop platform and riser (pipe) designs to survive the offshore environment. These tools are typically validated by scale model tests in facilities able to replicate real at-sea meteorological and ocean (metocean) conditions to provide the understanding and confidence to proceed to final design and full-scale fabrication. However, today’s offshore platforms (similar to and usually larger than those needed for OTEC applications) incorporate risers (or pipes) with diameters well under one meter. Secondly, the preferred construction method for large diameter OTEC CWPs is the use of composite materials, primarily a form of fiber-reinforced plastic (FRP). The use of these material results in relatively low pipe stiffness and large strains compared to steel construction. These factors suggest the need for further validation of offshore industry software tools. The purpose of this project was to validate the ability to model numerically the dynamic interaction between a large cold water-filled fiberglass pipe and a floating OTEC platform excited by metocean weather conditions using measurements from a scale model tested in an ocean basin test facility.

Solution coating of large-area organic semiconductor thin films with aligned single-crystalline domains

KAUST Repository

Diao, Ying

2013-06-02

Solution coating of organic semiconductors offers great potential for achieving low-cost manufacturing of large-area and flexible electronics. However, the rapid coating speed needed for industrial-scale production poses challenges to the control of thin-film morphology. Here, we report an approach - termed fluid-enhanced crystal engineering (FLUENCE) - that allows for a high degree of morphological control of solution-printed thin films. We designed a micropillar-patterned printing blade to induce recirculation in the ink for enhancing crystal growth, and engineered the curvature of the ink meniscus to control crystal nucleation. Using FLUENCE, we demonstrate the fast coating and patterning of millimetre-wide, centimetre-long, highly aligned single-crystalline organic semiconductor thin films. In particular, we fabricated thin films of 6,13-bis(triisopropylsilylethynyl) pentacene having non-equilibrium single-crystalline domains and an unprecedented average and maximum mobilities of 8.1±1.2 cm2 V-1 s -1 and 11 cm2 V-1 s-1. FLUENCE of organic semiconductors with non-equilibrium single-crystalline domains may find use in the fabrication of high-performance, large-area printed electronics. © 2013 Macmillan Publishers Limited. All rights reserved.

Experimental platforms in support of the ASTRID program: existing and planned facilities - 15126

International Nuclear Information System (INIS)

Gastaldi, O.; Rodriguez, G.; Ayrault, L.; Collard, B.; Dumesnil, J.; Dujet, F.; Journeau, C.; Latge, C.; Sanseigne, E.; Serre, F.; Tkatschenko, I.; Willermoz, G.

2015-01-01

The sodium cooled fast reactors (SFR) French program currently focused on the design of the ASTRID (Advanced Sodium Technological Reactor for Industrial Demonstration) reactor in different fields: energy conversion system, instrumentation for continuous monitoring, In Service Inspection and Repair, core design, fuel handling, thermo hydraulic, severe accidents, large flow electromagnetic pumps... Even if the French experimental prototype implies the development of innovative techniques, concepts and feedback of operations of SFRs are important, the new challenges coming from the objectives to meet GEN-IV requirements need some research and development. To achieve this goal, the generation four French SFR program includes the development of technological platforms with experimental facilities to develop and evaluate innovative options and also qualify some ASTRID specific components. The needs in terms of development, validation and qualification of techniques, components or systems to be used on ASTRID have been reviewed exhaustively in 2014. It allowed to consolidate or to precise the experimental purposes of the four CEA platforms regrouping technological facilities with different strategy of erection. PAPIRUS platform (largely already constructed) is dedicated to in-sodium experimental testing; GISEH platform (also largely already constructed) is devoted to water and air tests in support to hydraulic, thermal-hydraulic and fluid-structure interaction studies; CHEOPS platform (detailed studies and realization contract launched in 2014 aiming at commissioning and start up in 2018) deals with in sodium research and development and some qualifications requiring large scale; and last, PLINIUS-2 platform (commissioning and start up in 2019) concerns prototypic corium behavior studies in severe accident conditions and mitigation device qualification. This paper presents the four platforms and for each of them the experimental needs which are covered by their facilities

A universal and label-free impedimetric biosensing platform for discrimination of single nucleotide substitutions in long nucleic acid strands.

Science.gov (United States)

Mills, Dawn M; Martin, Christopher P; Armas, Stephanie M; Calvo-Marzal, Percy; Kolpashchikov, Dmitry M; Chumbimuni-Torres, Karin Y

2018-06-30

We report a label-free universal biosensing platform for highly selective detection of long nucleic acid strands. The sensor consists of an electrode-immobilized universal stem-loop (USL) probe and two adaptor strands that form a 4J structure in the presence of a specific DNA/RNA analyte. The sensor was characterized by electrochemical impedance spectroscopy (EIS) using K 3 [Fe(CN) 6 ]/K 4 [Fe(CN) 6 ] redox couple in solution. An increase in charge transfer resistance (R CT ) was observed upon 4J structure formation, the value of which depends on the analyte length. Cyclic voltammetry (CV) was used to further characterize the sensor and monitor the electrochemical reaction in conjunction with thickness measurements of the mixed DNA monolayer obtained using spectroscopic ellipsometry. In addition, the electron transfer was calculated at the electrode/electrolyte interface using a rotating disk electrode. Limits of detection in the femtomolar range were achieved for nucleic acid targets of different lengths (22 nt, 60 nt, 200 nt). The sensor produced only a background signal in the presence of single base mismatched analytes, even in hundred times excess in concentration. This label-free and highly selective biosensing platform is versatile and can be used for universal detection of nucleic acids of varied lengths which could revolutionize point of care diagnostics for applications such as bacterial or cancer screening. Copyright © 2018 Elsevier B.V. All rights reserved.

The 'cube' meta-model for the information system of large health sector organizations--a (platform neutral) mapping tool to integrate information system development with changing business functions and organizational development.

Science.gov (United States)

Balkányi, László

2002-01-01

To develop information systems (IS) in the changing environment of the health sector, a simple but throughout model, avoiding the techno-jargon of informatics, might be useful for the top management. A platform neutral, extensible, transparent conceptual model should be established. Limitations of current methods lead to a simple, but comprehensive mapping, in the form of a three-dimensional cube. The three 'orthogonal' views are (a) organization functionality, (b) organizational structures and (c) information technology. Each of the cube-sides is described according to its nature. This approach enables to define any kind of an IS component as a certain point/layer/domain of the cube and enables also the management to label all IS components independently form any supplier(s) and/or any specific platform. The model handles changes in organization structure, business functionality and the serving info-system independently form each other. Practical application extends to (a) planning complex, new ISs, (b) guiding development of multi-vendor, multi-site ISs, (c) supporting large-scale public procurement procedures and the contracting, implementation phase by establishing a platform neutral reference, (d) keeping an exhaustive inventory of an existing large-scale system, that handles non-tangible aspects of the IS.

Highly sensitive and multiplexed platforms for allergy diagnostics

Science.gov (United States)

Monroe, Margo R.

Allergy is a disorder of the immune system caused by an immune response to otherwise harmless environmental allergens. Currently 20% of the US population is allergic and 90% of pediatric patients and 60% of adult patients with asthma have allergies. These percentages have increased by 18.5% in the past decade, with predicted similar trends for the future. Here we design sensitive, multiplexed platforms to detect allergen-specific IgE using the Interferometric Reflectance Imaging Sensor (IRIS) for various clinical settings. A microarray platform for allergy diagnosis allows for testing of specific IgE sensitivity to a multitude of allergens, while requiring only small volumes of patient blood sample. However, conventional fluorescent microarray technology is limited by i) the variation of probe immobilization, which hinders the ability to make quantitative, assertive, and statistically relevant conclusions necessary in immunodiagnostics and ii) the use of fluorophore labels, which is not suitable for some clinical applications due to the tendency of fluorophores to stick to blood particulates and require daily calibration methods. This calibrated fluorescence enhancement (CaFE) method integrates the low magnification modality of IRIS with enhanced fluorescence sensing in order to directly correlate immobilized probe (major allergens) density to allergen-specific IgE in patient serum. However, this platform only operates in processed serum samples, which is not ideal for point of care testing. Thus, a high magnification modality of IRIS was adapted as an alternative allergy diagnostic platform to automatically discriminate and size single nanoparticles bound to specific IgE in unprocessed, characterized human blood and serum samples. These features make IRIS an ideal candidate for clinical and diagnostic applications, such a POC testing. The high magnification (nanoparticle counting) modality in conjunction with low magnification of IRIS in a combined instrument

An Open-Source Based ITS Platform

DEFF Research Database (Denmark)

Andersen, Ove; Krogh, Benjamin Bjerre; Torp, Kristian

2013-01-01

In this paper, a complete platform used to compute travel times from GPS data is described. Two approaches to computing travel time are proposed one based on points and one based on trips. Overall both approaches give reasonable results compared to existing manual estimated travel times. However......, the trip-based approach requires more GPS data and of a higher quality than the point-based approach. The platform has been completely implemented using open-source software. The main conclusion is that large quantity of GPS data can be managed, with a limited budget and that GPS data is a good source...... for estimating travel times, if enough data is available....

Remote sensing image segmentation based on Hadoop cloud platform

Science.gov (United States)

Li, Jie; Zhu, Lingling; Cao, Fubin

2018-01-01

To solve the problem that the remote sensing image segmentation speed is slow and the real-time performance is poor, this paper studies the method of remote sensing image segmentation based on Hadoop platform. On the basis of analyzing the structural characteristics of Hadoop cloud platform and its component MapReduce programming, this paper proposes a method of image segmentation based on the combination of OpenCV and Hadoop cloud platform. Firstly, the MapReduce image processing model of Hadoop cloud platform is designed, the input and output of image are customized and the segmentation method of the data file is rewritten. Then the Mean Shift image segmentation algorithm is implemented. Finally, this paper makes a segmentation experiment on remote sensing image, and uses MATLAB to realize the Mean Shift image segmentation algorithm to compare the same image segmentation experiment. The experimental results show that under the premise of ensuring good effect, the segmentation rate of remote sensing image segmentation based on Hadoop cloud Platform has been greatly improved compared with the single MATLAB image segmentation, and there is a great improvement in the effectiveness of image segmentation.

Quantum photonics hybrid integration platform

Energy Technology Data Exchange (ETDEWEB)

Murray, E.; Floether, F. F. [Cambridge Research Laboratory, Toshiba Research Europe Limited, 208 Science Park, Milton Road, Cambridge CB4 0GZ (United Kingdom); Cavendish Laboratory, University of Cambridge, J.J. Thomson Avenue, Cambridge CB3 0HE (United Kingdom); Ellis, D. J. P.; Meany, T.; Bennett, A. J., E-mail: anthony.bennet@crl.toshiba.co.uk; Shields, A. J. [Cambridge Research Laboratory, Toshiba Research Europe Limited, 208 Science Park, Milton Road, Cambridge CB4 0GZ (United Kingdom); Lee, J. P. [Cambridge Research Laboratory, Toshiba Research Europe Limited, 208 Science Park, Milton Road, Cambridge CB4 0GZ (United Kingdom); Engineering Department, University of Cambridge, 9 J. J. Thomson Avenue, Cambridge CB3 0FA (United Kingdom); Griffiths, J. P.; Jones, G. A. C.; Farrer, I.; Ritchie, D. A. [Cavendish Laboratory, University of Cambridge, J.J. Thomson Avenue, Cambridge CB3 0HE (United Kingdom)

2015-10-26

Fundamental to integrated photonic quantum computing is an on-chip method for routing and modulating quantum light emission. We demonstrate a hybrid integration platform consisting of arbitrarily designed waveguide circuits and single-photon sources. InAs quantum dots (QD) embedded in GaAs are bonded to a SiON waveguide chip such that the QD emission is coupled to the waveguide mode. The waveguides are SiON core embedded in a SiO{sub 2} cladding. A tuneable Mach Zehnder interferometer (MZI) modulates the emission between two output ports and can act as a path-encoded qubit preparation device. The single-photon nature of the emission was verified using the on-chip MZI as a beamsplitter in a Hanbury Brown and Twiss measurement.

The Core Interaction of Platforms: How Startups Connect Users and Producers

Directory of Open Access Journals (Sweden)

Heidi M. E. Korhonen

2017-09-01

Full Text Available The platform economy is disrupting innovation while presenting both opportunities and challenges for startups. Platforms support value creation between multiple participant groups, and this operationalization of an ecosystem’s value co-creation represents the “core interaction” of a platform. This article focuses on that core interaction and studies how startups connect producers and users in value-creating core interaction through digital platforms. The study is based on an analysis of 29 cases of platform startups interviewed at a leading European startup event. The studied startups were envisioning even millions of users and hundreds or thousands of producers co-creating value on their platforms. In such platform businesses, our results highlight the importance of attracting a large user pool, providing novel services to those users, offering a new market for producers, supporting the core interaction in various ways, and utilizing elements of the platform canvas – an adaptation of the business model canvas, which we have accommodated for platform-based business models – to accomplish these goals.

Towards a magnetoresistive platform for neural signal recording

Science.gov (United States)

Sharma, P. P.; Gervasoni, G.; Albisetti, E.; D'Ercoli, F.; Monticelli, M.; Moretti, D.; Forte, N.; Rocchi, A.; Ferrari, G.; Baldelli, P.; Sampietro, M.; Benfenati, F.; Bertacco, R.; Petti, D.

2017-05-01

A promising strategy to get deeper insight on brain functionalities relies on the investigation of neural activities at the cellular and sub-cellular level. In this framework, methods for recording neuron electrical activity have gained interest over the years. Main technological challenges are associated to finding highly sensitive detection schemes, providing considerable spatial and temporal resolution. Moreover, the possibility to perform non-invasive assays would constitute a noteworthy benefit. In this work, we present a magnetoresistive platform for the detection of the action potential propagation in neural cells. Such platform allows, in perspective, the in vitro recording of neural signals arising from single neurons, neural networks and brain slices.

Strategies for comparing gene expression profiles from different microarray platforms: application to a case-control experiment.

Science.gov (United States)

Severgnini, Marco; Bicciato, Silvio; Mangano, Eleonora; Scarlatti, Francesca; Mezzelani, Alessandra; Mattioli, Michela; Ghidoni, Riccardo; Peano, Clelia; Bonnal, Raoul; Viti, Federica; Milanesi, Luciano; De Bellis, Gianluca; Battaglia, Cristina

2006-06-01

Meta-analysis of microarray data is increasingly important, considering both the availability of multiple platforms using disparate technologies and the accumulation in public repositories of data sets from different laboratories. We addressed the issue of comparing gene expression profiles from two microarray platforms by devising a standardized investigative strategy. We tested this procedure by studying MDA-MB-231 cells, which undergo apoptosis on treatment with resveratrol. Gene expression profiles were obtained using high-density, short-oligonucleotide, single-color microarray platforms: GeneChip (Affymetrix) and CodeLink (Amersham). Interplatform analyses were carried out on 8414 common transcripts represented on both platforms, as identified by LocusLink ID, representing 70.8% and 88.6% of annotated GeneChip and CodeLink features, respectively. We identified 105 differentially expressed genes (DEGs) on CodeLink and 42 DEGs on GeneChip. Among them, only 9 DEGs were commonly identified by both platforms. Multiple analyses (BLAST alignment of probes with target sequences, gene ontology, literature mining, and quantitative real-time PCR) permitted us to investigate the factors contributing to the generation of platform-dependent results in single-color microarray experiments. An effective approach to cross-platform comparison involves microarrays of similar technologies, samples prepared by identical methods, and a standardized battery of bioinformatic and statistical analyses.

Large linear magnetoresistance and shubnikov-de hass oscillations in single crystals of YPdBi heusler topological insulators

KAUST Repository

Wang, Wenhong; Du, Yin; Xu, Guizhou; Zhang, Xiaoming; Liu, Enke; Liu, Zhongyuan; Shi, Youguo; Chen, Jinglan; Wu, Guangheng; Zhang, Xixiang

2013-01-01

We report the observation of a large linear magnetoresistance (MR) and Shubnikov-de Hass (SdH) quantum oscillations in single crystals of YPdBi Heusler topological insulators. Owning to the successfully obtained the high-quality YPdBi single crystals, large non-saturating linear MR of as high as 350% at 5K and over 120% at 300K under a moderate magnetic field of 7T is observed. In addition to the large, field-linear MR, the samples exhibit pronounced SdH quantum oscillations at low temperature. Analysis of the SdH data manifests that the high-mobility bulk electron carriers dominate the magnetotransport and are responsible for the observed large linear MR in YPdBi crystals. These findings imply that the Heusler-based topological insulators have superiorities for investigating the novel quantum transport properties and developing the potential applications.

Large linear magnetoresistance and shubnikov-de hass oscillations in single crystals of YPdBi heusler topological insulators

KAUST Repository

Wang, Wenhong

2013-07-12

We report the observation of a large linear magnetoresistance (MR) and Shubnikov-de Hass (SdH) quantum oscillations in single crystals of YPdBi Heusler topological insulators. Owning to the successfully obtained the high-quality YPdBi single crystals, large non-saturating linear MR of as high as 350% at 5K and over 120% at 300K under a moderate magnetic field of 7T is observed. In addition to the large, field-linear MR, the samples exhibit pronounced SdH quantum oscillations at low temperature. Analysis of the SdH data manifests that the high-mobility bulk electron carriers dominate the magnetotransport and are responsible for the observed large linear MR in YPdBi crystals. These findings imply that the Heusler-based topological insulators have superiorities for investigating the novel quantum transport properties and developing the potential applications.

Imaging large cohorts of single ion channels and their activity

Directory of Open Access Journals (Sweden)

Katia eHiersemenzel

2013-09-01

Full Text Available As calcium is the most important signaling molecule in neurons and secretory cells, amongst many other cell types, it follows that an understanding of calcium channels and their regulation of exocytosis is of vital importance. Calcium imaging using calcium dyes such as Fluo3, or FRET-based dyes that have been used widely has provided invaluable information, which combined with modeling has estimated the sub-types of channels responsible for triggering the exocytotic machinery as well as inferences about the relative distances away from vesicle fusion sites these molecules adopt. Importantly, new super-resolution microscopy techniques, combined with novel Ca2+ indicators and imaginative imaging approaches can now define directly the nanoscale locations of very large cohorts of single channel molecules in relation to single vesicles. With combinations of these techniques the activity of individual channels can be visualized and quantified using novel Ca2+ indicators. Fluorescently labeled specific channel toxins can also be used to localize endogenous assembled channel tetramers. Fluorescence lifetime imaging microscopy and other single-photon-resolution spectroscopic approaches offer the possibility to quantify protein-protein interactions between populations of channels and the SNARE protein machinery for the first time. Together with simultaneous electrophysiology, this battery of quantitative imaging techniques has the potential to provide unprecedented detail describing the locations, dynamic behaviours, interactions and conductance activities of many thousands of channel molecules and vesicles in living cells.

Large or small angle MSW from single right-handed neutrino dominance

International Nuclear Information System (INIS)

King, S.F

2000-01-01

In this talk we discuss a natural explanation of both neutrino mass hierarchies and large neutrino mixing angles, as required by the atmospheric neutrino data, in terms of a single right-handed neutrino giving the dominant contribution to the 23 block of the light effective neutrino matrix, and illustrate this mechanism in the framework of models with U(1) family symmetries. Sub-dominant contributions from other right-handed neutrinos are required to give small mass splittings appropriate to the MSW solution to the solar neutrino problem. We present three explicit examples for achieving the small angle MSW solution in the framework of U(1) family symmetry models containing three right-handed neutrinos, which can naturally describe all quark and lepton masses and mixing angles. In this talk we also extend the analysis to the large angle MSW solution

Synthesis and characterization of p-type boron-doped IIb diamond large single crystals

International Nuclear Information System (INIS)

Li Shang-Sheng; Li Xiao-Lei; Su Tai-Chao; Jia Xiao-Peng; Ma Hong-An; Huang Guo-Feng; Li Yong

2011-01-01

High-quality p-type boron-doped IIb diamond large single crystals are successfully synthesized by the temperature gradient method in a china-type cubic anvil high-pressure apparatus at about 5.5 GPa and 1600 K. The morphologies and surface textures of the synthetic diamond crystals with different boron additive quantities are characterized by using an optical microscope and a scanning electron microscope respectively. The impurities of nitrogen and boron in diamonds are detected by micro Fourier transform infrared technique. The electrical properties including resistivities, Hall coefficients, Hall mobilities and carrier densities of the synthesized samples are measured by a four-point probe and the Hall effect method. The results show that large p-type boron-doped diamond single crystals with few nitrogen impurities have been synthesized. With the increase of quantity of additive boron, some high-index crystal faces such as {113} gradually disappear, and some stripes and triangle pits occur on the crystal surface. This work is helpful for the further research and application of boron-doped semiconductor diamond. (cross-disciplinary physics and related areas of science and technology)

Hematological changes after single large dose half-body irradiation

International Nuclear Information System (INIS)

Herrmann, T.; Friedrich, S.; Jochem, I.; Eberhardt, H.J.; Koch, R.; Knorr, A.

1981-01-01

The determination of different peripheral blood parameters aimed at the study of side effects on the hematological cellular system following a 5 - 8 Gy single large dose half-body irradiation in 20 patients. Compared to the initial values the leukocytes between the 6. and 14., the thrombocytes between the 14. and 21. postirradiation day as well as the lymphocytes between 3 hours and 4 weeks postirradiation were significantly decreased without exhibiting complications such as hemorrhages or infections. The hemoglobin, hematocrit and reticulocyte values revealed but a slight decrease normalized within a 28 days postirradiation period. Transfusions were necessary when a tumor-caused anemia was present prior to irradiation. Changes in serum activity of aminotransferases and lactate dehydrogenase occured during the first hours after irradiation and were due to enzyme release from destroyed tumor cells

Product Platform Performance

DEFF Research Database (Denmark)

Munk, Lone

The aim of this research is to improve understanding of platform-based product development by studying platform performance in relation to internal effects in companies. Platform-based product development makes it possible to deliver product variety and at the same time reduce the needed resources...... engaging in platform-based product development. Similarly platform assessment criteria lack empirical verification regarding relevance and sufficiency. The thesis focuses on • the process of identifying and estimating internal effects, • verification of performance of product platforms, (i...... experienced representatives from the different life systems phase systems of the platform products. The effects are estimated and modeled within different scenarios, taking into account financial and real option aspects. The model illustrates and supports estimation and quantification of internal platform...

Massively Parallel, Molecular Analysis Platform Developed Using a CMOS Integrated Circuit With Biological Nanopores

Science.gov (United States)

Roever, Stefan

2012-01-01

A massively parallel, low cost molecular analysis platform will dramatically change the nature of protein, molecular and genomics research, DNA sequencing, and ultimately, molecular diagnostics. An integrated circuit (IC) with 264 sensors was fabricated using standard CMOS semiconductor processing technology. Each of these sensors is individually controlled with precision analog circuitry and is capable of single molecule measurements. Under electronic and software control, the IC was used to demonstrate the feasibility of creating and detecting lipid bilayers and biological nanopores using wild type α-hemolysin. The ability to dynamically create bilayers over each of the sensors will greatly accelerate pore development and pore mutation analysis. In addition, the noise performance of the IC was measured to be 30fA(rms). With this noise performance, single base detection of DNA was demonstrated using α-hemolysin. The data shows that a single molecule, electrical detection platform using biological nanopores can be operationalized and can ultimately scale to millions of sensors. Such a massively parallel platform will revolutionize molecular analysis and will completely change the field of molecular diagnostics in the future.

Visualisation methods for large provenance collections in data-intensive collaborative platforms

Science.gov (United States)

Spinuso, Alessandro; Fligueira, Rosa; Atkinson, Malcolm; Gemuend, Andre

2016-04-01

This work investigates improving the methods of visually representing provenance information in the context of modern data-driven scientific research. It explores scenarios where data-intensive workflows systems are serving communities of researchers within collaborative environments, supporting the sharing of data and methods, and offering a variety of computation facilities, including HPC, HTC and Cloud. It focuses on the exploration of big-data visualization techniques aiming at producing comprehensive and interactive views on top of large and heterogeneous provenance data. The same approach is applicable to control-flow and data-flow workflows or to combinations of the two. This flexibility is achieved using the W3C-PROV recommendation as a reference model, especially its workflow oriented profiles such as D-PROV (Messier et al. 2013). Our implementation is based on the provenance records produced by the dispel4py data-intensive processing library (Filgueira et al. 2015). dispel4py is an open-source Python framework for describing abstract stream-based workflows for distributed data-intensive applications, developed during the VERCE project. dispel4py enables scientists to develop their scientific methods and applications on their laptop and then run them at scale on a wide range of e-Infrastructures (Cloud, Cluster, etc.) without making changes. Users can therefore focus on designing their workflows at an abstract level, describing actions, input and output streams, and how they are connected. The dispel4py system then maps these descriptions to the enactment platforms, such as MPI, Storm, multiprocessing. It provides a mechanism which allows users to determine the provenance information to be collected and to analyze it at runtime. For this work we consider alternative visualisation methods for provenance data, from infinite lists and localised interactive graphs, to radial-views. The latter technique has been positively explored in many fields, from text

A robotics platform for automated batch fabrication of high density, microfluidics-based DNA microarrays, with applications to single cell, multiplex assays of secreted proteins

Science.gov (United States)

Ahmad, Habib; Sutherland, Alex; Shin, Young Shik; Hwang, Kiwook; Qin, Lidong; Krom, Russell-John; Heath, James R.

2011-09-01

Microfluidics flow-patterning has been utilized for the construction of chip-scale miniaturized DNA and protein barcode arrays. Such arrays have been used for specific clinical and fundamental investigations in which many proteins are assayed from single cells or other small sample sizes. However, flow-patterned arrays are hand-prepared, and so are impractical for broad applications. We describe an integrated robotics/microfluidics platform for the automated preparation of such arrays, and we apply it to the batch fabrication of up to eighteen chips of flow-patterned DNA barcodes. The resulting substrates are comparable in quality with hand-made arrays and exhibit excellent substrate-to-substrate consistency. We demonstrate the utility and reproducibility of robotics-patterned barcodes by utilizing two flow-patterned chips for highly parallel assays of a panel of secreted proteins from single macrophage cells.

A robotics platform for automated batch fabrication of high density, microfluidics-based DNA microarrays, with applications to single cell, multiplex assays of secreted proteins.

Science.gov (United States)

Ahmad, Habib; Sutherland, Alex; Shin, Young Shik; Hwang, Kiwook; Qin, Lidong; Krom, Russell-John; Heath, James R

2011-09-01

Microfluidics flow-patterning has been utilized for the construction of chip-scale miniaturized DNA and protein barcode arrays. Such arrays have been used for specific clinical and fundamental investigations in which many proteins are assayed from single cells or other small sample sizes. However, flow-patterned arrays are hand-prepared, and so are impractical for broad applications. We describe an integrated robotics/microfluidics platform for the automated preparation of such arrays, and we apply it to the batch fabrication of up to eighteen chips of flow-patterned DNA barcodes. The resulting substrates are comparable in quality with hand-made arrays and exhibit excellent substrate-to-substrate consistency. We demonstrate the utility and reproducibility of robotics-patterned barcodes by utilizing two flow-patterned chips for highly parallel assays of a panel of secreted proteins from single macrophage cells. © 2011 American Institute of Physics

Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia.

Science.gov (United States)

Zhao, Xi; Huang, Xiaomeng; Wang, Xinmei; Wu, Yun; Eisfeld, Ann-Kathrin; Schwind, Sebastian; Gallego-Perez, Daniel; Boukany, Pouyan E; Marcucci, Guido I; Lee, Ly James

2015-12-01

A living cell interrogation platform based on nanochannel electroporation is demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi-target analysis and stimulus-response analysis by sequential deliveries. The unique platform possesses a great potential to the comprehensive and lysis-free nucleic acid analysis on rare or hard-to-transfect cells.

Novel Biochip Platform for Nucleic Acid Analysis

Directory of Open Access Journals (Sweden)

Juan J. Diaz-Mochon

2012-06-01

Full Text Available This manuscript describes the use of a novel biochip platform for the rapid analysis/identification of nucleic acids, including DNA and microRNAs, with very high specificity. This approach combines a unique dynamic chemistry approach for nucleic acid testing and analysis developed by DestiNA Genomics with the STMicroelectronics In-Check platform, which comprises two microfluidic optimized and independent PCR reaction chambers, and a sequential microarray area for nucleic acid capture and identification by fluorescence. With its compact bench-top “footprint” requiring only a single technician to operate, the biochip system promises to transform and expand routine clinical diagnostic testing and screening for genetic diseases, cancers, drug toxicology and heart disease, as well as employment in the emerging companion diagnostics market.

A Single-Molecule Barcoding System using Nanoslits for DNA Analysis

Science.gov (United States)

Jo, Kyubong; Schramm, Timothy M.; Schwartz, David C.

Single DNA molecule approaches are playing an increasingly central role in the analytical genomic sciences because single molecule techniques intrinsically provide individualized measurements of selected molecules, free from the constraints of bulk techniques, which blindly average noise and mask the presence of minor analyte components. Accordingly, a principal challenge that must be addressed by all single molecule approaches aimed at genome analysis is how to immobilize and manipulate DNA molecules for measurements that foster construction of large, biologically relevant data sets. For meeting this challenge, this chapter discusses an integrated approach for microfabricated and nanofabricated devices for the manipulation of elongated DNA molecules within nanoscale geometries. Ideally, large DNA coils stretch via nanoconfinement when channel dimensions are within tens of nanometers. Importantly, stretched, often immobilized, DNA molecules spanning hundreds of kilobase pairs are required by all analytical platforms working with large genomic substrates because imaging techniques acquire sequence information from molecules that normally exist in free solution as unrevealing random coils resembling floppy balls of yarn. However, nanoscale devices fabricated with sufficiently small dimensions fostering molecular stretching make these devices impractical because of the requirement of exotic fabrication technologies, costly materials, and poor operational efficiencies. In this chapter, such problems are addressed by discussion of a new approach to DNA presentation and analysis that establishes scaleable nanoconfinement conditions through reduction of ionic strength; stiffening DNA molecules thus enabling their arraying for analysis using easily fabricated devices that can also be mass produced. This new approach to DNA nanoconfinement is complemented by the development of a novel labeling scheme for reliable marking of individual molecules with fluorochrome labels

Horizontally scaling dCache SRM with the Terracotta platform

International Nuclear Information System (INIS)

Perelmutov, T; Crawford, M; Moibenko, A; Oleynik, G

2011-01-01

The dCache disk caching file system has been chosen by a majority of LHC experiments' Tier 1 centers for their data storage needs. It is also deployed at many Tier 2 centers. The Storage Resource Manager (SRM) is a standardized grid storage interface and a single point of remote entry into dCache, and hence is a critical component. SRM must scale to increasing transaction rates and remain resilient against changing usage patterns. The initial implementation of the SRM service in dCache suffered from an inability to support clustered deployment, and its performance was limited by the hardware of a single node. Using the Terracotta platform[l], we added the ability to horizontally scale the dCache SRM service to run on multiple nodes in a cluster configuration, coupled with network load balancing. This gives site administrators the ability to increase the performance and reliability of SRM service to face the ever-increasing requirements of LHC data handling. In this paper we will describe the previous limitations of the architecture SRM server and how the Terracotta platform allowed us to readily convert single node service into a highly scalable clustered application.

Horizontally scaling dChache SRM with the Terracotta platform

International Nuclear Information System (INIS)

Perelmutov, T.; Crawford, M.; Moibenko, A.; Oleynik, G.

2011-01-01

The dCache disk caching file system has been chosen by a majority of LHC experiments Tier 1 centers for their data storage needs. It is also deployed at many Tier 2 centers. The Storage Resource Manager (SRM) is a standardized grid storage interface and a single point of remote entry into dCache, and hence is a critical component. SRM must scale to increasing transaction rates and remain resilient against changing usage patterns. The initial implementation of the SRM service in dCache suffered from an inability to support clustered deployment, and its performance was limited by the hardware of a single node. Using the Terracotta platform, we added the ability to horizontally scale the dCache SRM service to run on multiple nodes in a cluster configuration, coupled with network load balancing. This gives site administrators the ability to increase the performance and reliability of SRM service to face the ever-increasing requirements of LHC data handling. In this paper we will describe the previous limitations of the architecture SRM server and how the Terracotta platform allowed us to readily convert single node service into a highly scalable clustered application.

Health Information Research Platform (HIReP)--an architecture pattern.

Science.gov (United States)

Schreiweis, Björn; Schneider, Gerd; Eichner, Theresia; Bergh, Björn; Heinze, Oliver

2014-01-01

Secondary use or single source is still far from routine in healthcare, although lots of data are available either structured or unstructured. As data are stored in multiple systems, using them for biomedical research is difficult. Clinical data warehouses already help overcoming this issue, but currently they are only used for certain parts of biomedical research. A comprehensive research platform based on a generic architecture pattern could increase the benefits of existing data warehouses for both patient care and research by meeting two objectives: serving as a so called single point-of-truth and acting as a mediator between them strengthening interaction and close collaboration. Another effect is to reduce boundaries for the implementation of data warehouses. Taking further settings into account the architecture of a clinical data warehouse supporting patient care and biomedical research needs to be integrated with biomaterial banks and other sources. This work provides a solution conceptualizing a comprehensive architecture pattern of a Health Information Research Platform (HIReP) derived from use cases of the patient care and biomedical research domain. It serves as single IT infrastructure providing solutions for any type of use case.

Crowd-Sourcing the Aesthetics of Platform Games

DEFF Research Database (Denmark)

Shaker, Noor; Yannakakis, Georgios N.; Togelius, Julian

2014-01-01

What are the aesthetics of platform games and what makes a platform level engaging, challenging and/or frustrating? We attempt to answer such questions through mining a large-set of crowd-sourced gameplay data of a clone of the classic platform game Super Mario Bros. The data consists of 40 short...... game levels that differ along six key level design parameters. Collectively, these levels are played 1560 times over the Internet and the perceived experience is annotated by experiment participants via self-reported ranking (pairwise preferences). Given the wealth of this crowd-sourced data, as all...... details about players’ in-game behaviour are logged, the problem becomes one of extracting meaningful numerical features at the appropriate level of abstraction for the construction of generic computational models of player experience and, thereby, game aesthetics. We explore dissimilar types of features...

The Platformization of the Web: Making Web Data Platform Ready

NARCIS (Netherlands)

Helmond, A.

2015-01-01

In this article, I inquire into Facebook’s development as a platform by situating it within the transformation of social network sites into social media platforms. I explore this shift with a historical perspective on, what I refer to as, platformization, or the rise of the platform as the dominant

A hybrid approach to device integration on a genetic analysis platform

International Nuclear Information System (INIS)

Brennan, Des; Justice, John; Aherne, Margaret; Galvin, Paul; Jary, Dorothee; Kurg, Ants; Berik, Evgeny; Macek, Milan

2012-01-01

Point-of-care (POC) systems require significant component integration to implement biochemical protocols associated with molecular diagnostic assays. Hybrid platforms where discrete components are combined in a single platform are a suitable approach to integration, where combining multiple device fabrication steps on a single substrate is not possible due to incompatible or costly fabrication steps. We integrate three devices each with a specific system functionality: (i) a silicon electro-wetting-on-dielectric (EWOD) device to move and mix sample and reagent droplets in an oil phase, (ii) a polymer microfluidic chip containing channels and reservoirs and (iii) an aqueous phase glass microarray for fluorescence microarray hybridization detection. The EWOD device offers the possibility of fully integrating on-chip sample preparation using nanolitre sample and reagent volumes. A key challenge is sample transfer from the oil phase EWOD device to the aqueous phase microarray for hybridization detection. The EWOD device, waveguide performance and functionality are maintained during the integration process. An on-chip biochemical protocol for arrayed primer extension (APEX) was implemented for single nucleotide polymorphism (SNiP) analysis. The prepared sample is aspirated from the EWOD oil phase to the aqueous phase microarray for hybridization. A bench-top instrumentation system was also developed around the integrated platform to drive the EWOD electrodes, implement APEX sample heating and image the microarray after hybridization. (paper)

Can Effective Field Theory of inflation generate large tensor-to-scalar ratio within Randall–Sundrum single braneworld?

International Nuclear Information System (INIS)

Choudhury, Sayantan

2015-01-01

In this paper my prime objective is to explain the generation of large tensor-to-scalar ratio from the single field sub-Planckian inflationary paradigm within Randall–Sundrum (RS) single braneworld scenario in a model independent fashion. By explicit computation I have shown that the effective field theory prescription of brane inflation within RS single brane setup is consistent with sub-Planckian excursion of the inflaton field, which will further generate large value of tensor-to-scalar ratio, provided the energy density for inflaton degrees of freedom is high enough compared to the brane tension in high energy regime. Finally, I have mentioned the stringent theoretical constraint on positive brane tension, cut-off of the quantum gravity scale and bulk cosmological constant to get sub-Planckian field excursion along with large tensor-to-scalar ratio as recently observed by BICEP2 or at least generates the tensor-to-scalar ratio consistent with the upper bound of Planck (2013 and 2015) data and Planck+BICEP2+Keck Array joint constraint

Using and Designing Platforms for In Vivo Education Experiments

OpenAIRE

Williams, Joseph Jay; Ostrow, Korinn; Xiong, Xiaolu; Glassman, Elena; Kim, Juho; Maldonado, Samuel G.; Li, Na; Reich, Justin; Hefferman, Neil

2015-01-01

In contrast to typical laboratory experiments, the everyday use of online educational resources by large populations and the prevalence of software infrastructure for A/B testing leads us to consider how platforms can embed in vivo experiments that do not merely support research, but ensure practical improvements to their educational components. Examples are presented of randomized experimental comparisons conducted by subsets of the authors in three widely used online educational platforms K...

Large Out-of-Plane Displacement Bistable Electromagnetic Microswitch on a Single Wafer.

Science.gov (United States)

Miao, Xiaodan; Dai, Xuhan; Huang, Yi; Ding, Guifu; Zhao, Xiaolin

2016-05-05

This paper presents a bistable microswitch fully batch-fabricated on a single glass wafer, comprising of a microactuator, a signal transformer, a microspring and a permanent magnet. The bistable mechanism of the microswitch with large displacement of 160 μm depends on the balance of the magnetic force and elastic force. Both the magnetic force and elastic force were optimized by finite-element simulation to predict the reliable of the device. The prototype was fabricated and characterized. By utilizing thick laminated photoresist sacrificial layer, the large displacement was obtained to ensure the insulation of the microswitch. The testing results show that the microswitch realized the bistable mechanism at a 3-5 V input voltage and closed in 0.96 ms, which verified the simulation.

Assessing the validity of single-item life satisfaction measures: results from three large samples.

Science.gov (United States)

Cheung, Felix; Lucas, Richard E

2014-12-01

The present paper assessed the validity of single-item life satisfaction measures by comparing single-item measures to the Satisfaction with Life Scale (SWLS)-a more psychometrically established measure. Two large samples from Washington (N = 13,064) and Oregon (N = 2,277) recruited by the Behavioral Risk Factor Surveillance System and a representative German sample (N = 1,312) recruited by the Germany Socio-Economic Panel were included in the present analyses. Single-item life satisfaction measures and the SWLS were correlated with theoretically relevant variables, such as demographics, subjective health, domain satisfaction, and affect. The correlations between the two life satisfaction measures and these variables were examined to assess the construct validity of single-item life satisfaction measures. Consistent across three samples, single-item life satisfaction measures demonstrated substantial degree of criterion validity with the SWLS (zero-order r = 0.62-0.64; disattenuated r = 0.78-0.80). Patterns of statistical significance for correlations with theoretically relevant variables were the same across single-item measures and the SWLS. Single-item measures did not produce systematically different correlations compared to the SWLS (average difference = 0.001-0.005). The average absolute difference in the magnitudes of the correlations produced by single-item measures and the SWLS was very small (average absolute difference = 0.015-0.042). Single-item life satisfaction measures performed very similarly compared to the multiple-item SWLS. Social scientists would get virtually identical answer to substantive questions regardless of which measure they use.

Proba-V Mission Exploitation Platform

Science.gov (United States)

Goor, Erwin; Dries, Jeroen

2017-04-01

VITO and partners developed the Proba-V Mission Exploitation Platform (MEP) as an end-to-end solution to drastically improve the exploitation of the Proba-V (a Copernicus contributing mission) EO-data archive (http://proba-v.vgt.vito.be/), the past mission SPOT-VEGETATION and derived vegetation parameters by researchers, service providers and end-users. The analysis of time series of data (+1PB) is addressed, as well as the large scale on-demand processing of near real-time data on a powerful and scalable processing environment. Furthermore data from the Copernicus Global Land Service is in scope of the platform. From November 2015 an operational Proba-V MEP environment, as an ESA operation service, is gradually deployed at the VITO data center with direct access to the complete data archive. Since autumn 2016 the platform is operational and yet several applications are released to the users, e.g. - A time series viewer, showing the evolution of Proba-V bands and derived vegetation parameters from the Copernicus Global Land Service for any area of interest. - Full-resolution viewing services for the complete data archive. - On-demand processing chains on a powerfull Hadoop/Spark backend e.g. for the calculation of N-daily composites. - Virtual Machines can be provided with access to the data archive and tools to work with this data, e.g. various toolboxes (GDAL, QGIS, GrassGIS, SNAP toolbox, …) and support for R and Python. This allows users to immediately work with the data without having to install tools or download data, but as well to design, debug and test applications on the platform. - A prototype of jupyter Notebooks is available with some examples worked out to show the potential of the data. Today the platform is used by several third party projects to perform R&D activities on the data, and to develop/host data analysis toolboxes. In parallel the platform is further improved and extended. From the MEP PROBA-V, access to Sentinel-2 and landsat data will

A large collapsed-state RNA can exhibit simple exponential single-molecule dynamics.

Science.gov (United States)

Smith, Glenna J; Lee, Kang Taek; Qu, Xiaohui; Xie, Zheng; Pesic, Jelena; Sosnick, Tobin R; Pan, Tao; Scherer, Norbert F

2008-05-09

The process of large RNA folding is believed to proceed from many collapsed structures to a unique functional structure requiring precise organization of nucleotides. The diversity of possible structures and stabilities of large RNAs could result in non-exponential folding kinetics (e.g. stretched exponential) under conditions where the molecules have not achieved their native state. We describe a single-molecule fluorescence resonance energy transfer (FRET) study of the collapsed-state region of the free energy landscape of the catalytic domain of RNase P RNA from Bacillus stearothermophilus (C(thermo)). Ensemble measurements have shown that this 260 residue RNA folds cooperatively to its native state at >or=1 mM Mg(2+), but little is known about the conformational dynamics at lower ionic strength. Our measurements of equilibrium conformational fluctuations reveal simple exponential kinetics that reflect a small number of discrete states instead of the expected inhomogeneous dynamics. The distribution of discrete dwell times, collected from an "ensemble" of 300 single molecules at each of a series of Mg(2+) concentrations, fit well to a double exponential, which indicates that the RNA conformational changes can be described as a four-state system. This finding is somewhat unexpected under [Mg(2+)] conditions in which this RNA does not achieve its native state. Observation of discrete well-defined conformations in this large RNA that are stable on the seconds timescale at low [Mg(2+)] (<0.1 mM) suggests that even at low ionic strength, with a tremendous number of possible (weak) interactions, a few critical interactions may produce deep energy wells that allow for rapid averaging of motions within each well, and yield kinetics that are relatively simple.

MiSeq: A Next Generation Sequencing Platform for Genomic Analysis.

Science.gov (United States)

Ravi, Rupesh Kanchi; Walton, Kendra; Khosroheidari, Mahdieh

2018-01-01

MiSeq, Illumina's integrated next generation sequencing instrument, uses reversible-terminator sequencing-by-synthesis technology to provide end-to-end sequencing solutions. The MiSeq instrument is one of the smallest benchtop sequencers that can perform onboard cluster generation, amplification, genomic DNA sequencing, and data analysis, including base calling, alignment and variant calling, in a single run. It performs both single- and paired-end runs with adjustable read lengths from 1 × 36 base pairs to 2 × 300 base pairs. A single run can produce output data of up to 15 Gb in as little as 4 h of runtime and can output up to 25 M single reads and 50 M paired-end reads. Thus, MiSeq provides an ideal platform for rapid turnaround time. MiSeq is also a cost-effective tool for various analyses focused on targeted gene sequencing (amplicon sequencing and target enrichment), metagenomics, and gene expression studies. For these reasons, MiSeq has become one of the most widely used next generation sequencing platforms. Here, we provide a protocol to prepare libraries for sequencing using the MiSeq instrument and basic guidelines for analysis of output data from the MiSeq sequencing run.

Emerging heterogeneous integrated photonic platforms on silicon

Directory of Open Access Journals (Sweden)

Fathpour Sasan

2015-05-01

Full Text Available Silicon photonics has been established as a mature and promising technology for optoelectronic integrated circuits, mostly based on the silicon-on-insulator (SOI waveguide platform. However, not all optical functionalities can be satisfactorily achieved merely based on silicon, in general, and on the SOI platform, in particular. Long-known shortcomings of silicon-based integrated photonics are optical absorption (in the telecommunication wavelengths and feasibility of electrically-injected lasers (at least at room temperature. More recently, high two-photon and free-carrier absorptions required at high optical intensities for third-order optical nonlinear effects, inherent lack of second-order optical nonlinearity, low extinction ratio of modulators based on the free-carrier plasma effect, and the loss of the buried oxide layer of the SOI waveguides at mid-infrared wavelengths have been recognized as other shortcomings. Accordingly, several novel waveguide platforms have been developing to address these shortcomings of the SOI platform. Most of these emerging platforms are based on heterogeneous integration of other material systems on silicon substrates, and in some cases silicon is integrated on other substrates. Germanium and its binary alloys with silicon, III–V compound semiconductors, silicon nitride, tantalum pentoxide and other high-index dielectric or glass materials, as well as lithium niobate are some of the materials heterogeneously integrated on silicon substrates. The materials are typically integrated by a variety of epitaxial growth, bonding, ion implantation and slicing, etch back, spin-on-glass or other techniques. These wide range of efforts are reviewed here holistically to stress that there is no pure silicon or even group IV photonics per se. Rather, the future of the field of integrated photonics appears to be one of heterogenization, where a variety of different materials and waveguide platforms will be used for

A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions.

Science.gov (United States)

Schlecht, Ulrich; Liu, Zhimin; Blundell, Jamie R; St Onge, Robert P; Levy, Sasha F

2017-05-25

Several large-scale efforts have systematically catalogued protein-protein interactions (PPIs) of a cell in a single environment. However, little is known about how the protein interactome changes across environmental perturbations. Current technologies, which assay one PPI at a time, are too low throughput to make it practical to study protein interactome dynamics. Here, we develop a highly parallel protein-protein interaction sequencing (PPiSeq) platform that uses a novel double barcoding system in conjunction with the dihydrofolate reductase protein-fragment complementation assay in Saccharomyces cerevisiae. PPiSeq detects PPIs at a rate that is on par with current assays and, in contrast with current methods, quantitatively scores PPIs with enough accuracy and sensitivity to detect changes across environments. Both PPI scoring and the bulk of strain construction can be performed with cell pools, making the assay scalable and easily reproduced across environments. PPiSeq is therefore a powerful new tool for large-scale investigations of dynamic PPIs.

Mobile application platform heterogeneity: Android vs Windows phone vs iOS vs Firefox OS

OpenAIRE

Gronli, T-M; Hansen, J; Ghinea, G; Younas, M

2014-01-01

Modern smartphones have a rich spectrum of increasingly sophisticated features, opening opportunities for software-led innovation. Of the large number of platforms to develop new software on, in this paper we look closely at three platforms identified as market leaders for the smartphone market by Gartner Group in 2013 and one platform, Firefox OS, representing a new paradigm for operating systems based on web technologies. We compare the platforms in several different categories, such as sof...

The big data processing platform for intelligent agriculture

Science.gov (United States)

Huang, Jintao; Zhang, Lichen

2017-08-01

Big data technology is another popular technology after the Internet of Things and cloud computing. Big data is widely used in many fields such as social platform, e-commerce, and financial analysis and so on. Intelligent agriculture in the course of the operation will produce large amounts of data of complex structure, fully mining the value of these data for the development of agriculture will be very meaningful. This paper proposes an intelligent data processing platform based on Storm and Cassandra to realize the storage and management of big data of intelligent agriculture.

Hybrid Geometric Calibration Method for Multi-Platform Spaceborne SAR Image with Sparse Gcps

Science.gov (United States)

Lv, G.; Tang, X.; Ai, B.; Li, T.; Chen, Q.

2018-04-01

Geometric calibration is able to provide high-accuracy geometric coordinates of spaceborne SAR image through accurate geometric parameters in the Range-Doppler model by ground control points (GCPs). However, it is very difficult to obtain GCPs that covering large-scale areas, especially in the mountainous regions. In addition, the traditional calibration method is only used for single platform SAR images and can't support the hybrid geometric calibration for multi-platform images. To solve the above problems, a hybrid geometric calibration method for multi-platform spaceborne SAR images with sparse GCPs is proposed in this paper. First, we calibrate the master image that contains GCPs. Secondly, the point tracking algorithm is used to obtain the tie points (TPs) between the master and slave images. Finally, we calibrate the slave images using TPs as the GCPs. We take the Beijing-Tianjin- Hebei region as an example to study SAR image hybrid geometric calibration method using 3 TerraSAR-X images, 3 TanDEM-X images and 5 GF-3 images covering more than 235 kilometers in the north-south direction. Geometric calibration of all images is completed using only 5 GCPs. The GPS data extracted from GNSS receiver are used to assess the plane accuracy after calibration. The results after geometric calibration with sparse GCPs show that the geometric positioning accuracy is 3 m for TSX/TDX images and 7.5 m for GF-3 images.

Single-Photon Technologies Based on Quantum-Dots in Photonic Crystals

DEFF Research Database (Denmark)

Lehmann, Tau Bernstorff

-photon purity under quasi-resonantexcitation. Furthermore the waveguide based platform demonstrates indistinguishable single-photonsat timescales up to 13 ns.A setup for active demultiplexing of single-photons to a three-fold single-photon state is proposed.Using a fast electro-optical modulator, single...

Off-line mapping of multi-rate dependent task sets to many-core platforms

DEFF Research Database (Denmark)

Puffitsch, Wolfgang; Noulard, Eric; Pagetti, Claire

2015-01-01

This paper presents an approach to execute safety-critical applications on multi- and many-core processors in a predictable manner. We investigate three concrete platforms: the Intel Single-chip Cloud Computer, the Texas Instruments TMS320C6678 and the Tilera TILEmpower-Gx36. We define an execution...... model to safely execute dependent periodic task sets on these platforms. The four rules of the execution model entail that an off-line mapping of the application to the platform must be computed. The paper details our approach to automatically compute a valid mapping. Furthermore, we evaluate our...

Tethered elevator and platforms as space station facilities: Systems studies and demonstrative experiments

Science.gov (United States)

1986-01-01

Several key concepts of the science and applications tethered platforms were studied. Some conclusions reached are herein listed. Tether elevator and platform could improve the space station scientific and applicative capabilities. The space elevator presents unique characteristics as microgravity facility and as a tethered platform servicing vehicle. Pointing platforms could represent a new kind of observation facility for large class of payloads. The dynamical, control and technological complexity of these concepts advised demonstrative experiments. The on-going tethered satellite system offers the opportunity to perform such experiments. And feasibility studies are in progress.

Assessing the Validity of Single-item Life Satisfaction Measures: Results from Three Large Samples

Science.gov (United States)

Cheung, Felix; Lucas, Richard E.

2014-01-01

Purpose The present paper assessed the validity of single-item life satisfaction measures by comparing single-item measures to the Satisfaction with Life Scale (SWLS) - a more psychometrically established measure. Methods Two large samples from Washington (N=13,064) and Oregon (N=2,277) recruited by the Behavioral Risk Factor Surveillance System (BRFSS) and a representative German sample (N=1,312) recruited by the Germany Socio-Economic Panel (GSOEP) were included in the present analyses. Single-item life satisfaction measures and the SWLS were correlated with theoretically relevant variables, such as demographics, subjective health, domain satisfaction, and affect. The correlations between the two life satisfaction measures and these variables were examined to assess the construct validity of single-item life satisfaction measures. Results Consistent across three samples, single-item life satisfaction measures demonstrated substantial degree of criterion validity with the SWLS (zero-order r = 0.62 – 0.64; disattenuated r = 0.78 – 0.80). Patterns of statistical significance for correlations with theoretically relevant variables were the same across single-item measures and the SWLS. Single-item measures did not produce systematically different correlations compared to the SWLS (average difference = 0.001 – 0.005). The average absolute difference in the magnitudes of the correlations produced by single-item measures and the SWLS were very small (average absolute difference = 0.015 −0.042). Conclusions Single-item life satisfaction measures performed very similarly compared to the multiple-item SWLS. Social scientists would get virtually identical answer to substantive questions regardless of which measure they use. PMID:24890827

Cross-Platform Technologies

Directory of Open Access Journals (Sweden)

Maria Cristina ENACHE

2017-04-01

Full Text Available Cross-platform - a concept becoming increasingly used in recent years especially in the development of mobile apps, but this consistently over time and in the development of conventional desktop applications. The notion of cross-platform software (multi-platform or platform-independent refers to a software application that can run on more than one operating system or computing architecture. Thus, a cross-platform application can operate independent of software or hardware platform on which it is execute. As a generic definition presents a wide range of meanings for purposes of this paper we individualize this definition as follows: we will reduce the horizon of meaning and we use functionally following definition: a cross-platform application is a software application that can run on more than one operating system (desktop or mobile identical or in a similar way.

Platform Performance and Challenges - using Platforms in Lego Company

DEFF Research Database (Denmark)

Munk, Lone; Mortensen, Niels Henrik

2009-01-01

needs focus on the incentive of using the platform. This problem lacks attention in literature, as well as industry, where assessment criteria do not cover this aspect. Therefore, we recommend including user incentive in platform assessment criteria to these challenges. Concrete solution elements...... ensuring user incentive in platforms is an object for future research...

X-CHIP: an integrated platform for high-throughput protein crystallization and on-the-chip X-ray diffraction data collection

International Nuclear Information System (INIS)

Kisselman, Gera; Qiu, Wei; Romanov, Vladimir; Thompson, Christine M.; Lam, Robert; Battaile, Kevin P.; Pai, Emil F.; Chirgadze, Nickolay Y.

2011-01-01

The X-CHIP (X-ray Crystallography High-throughput Integrated Platform) is a novel microchip that has been developed to combine multiple steps of the crystallographic pipeline from crystallization to diffraction data collection on a single device to streamline the entire process. The X-CHIP (X-ray Crystallization High-throughput Integrated Platform) is a novel microchip that has been developed to combine multiple steps of the crystallographic pipeline from crystallization to diffraction data collection on a single device to streamline the entire process. The system has been designed for crystallization condition screening, visual crystal inspection, initial X-ray screening and data collection in a high-throughput fashion. X-ray diffraction data acquisition can be performed directly on-the-chip at room temperature using an in situ approach. The capabilities of the chip eliminate the necessity for manual crystal handling and cryoprotection of crystal samples, while allowing data collection from multiple crystals in the same drop. This technology would be especially beneficial for projects with large volumes of data, such as protein-complex studies and fragment-based screening. The platform employs hydrophilic and hydrophobic concentric ring surfaces on a miniature plate transparent to visible light and X-rays to create a well defined and stable microbatch crystallization environment. The results of crystallization and data-collection experiments demonstrate that high-quality well diffracting crystals can be grown and high-resolution diffraction data sets can be collected using this technology. Furthermore, the quality of a single-wavelength anomalous dispersion data set collected with the X-CHIP at room temperature was sufficient to generate interpretable electron-density maps. This technology is highly resource-efficient owing to the use of nanolitre-scale drop volumes. It does not require any modification for most in-house and synchrotron beamline systems and offers

X-CHIP: an integrated platform for high-throughput protein crystallization and on-the-chip X-ray diffraction data collection

Energy Technology Data Exchange (ETDEWEB)

Kisselman, Gera; Qiu, Wei; Romanov, Vladimir; Thompson, Christine M.; Lam, Robert [Ontario Cancer Institute, Princess Margaret Hospital, University Health Network, Toronto, Ontario M5G 2C4 (Canada); Battaile, Kevin P. [Argonne National Laboratory, Argonne, Illinois 60439 (United States); Pai, Emil F.; Chirgadze, Nickolay Y., E-mail: nchirgad@uhnresearch.ca [Ontario Cancer Institute, Princess Margaret Hospital, University Health Network, Toronto, Ontario M5G 2C4 (Canada); University of Toronto, Toronto, Ontario M5S 1A8 (Canada)

2011-06-01

The X-CHIP (X-ray Crystallography High-throughput Integrated Platform) is a novel microchip that has been developed to combine multiple steps of the crystallographic pipeline from crystallization to diffraction data collection on a single device to streamline the entire process. The X-CHIP (X-ray Crystallization High-throughput Integrated Platform) is a novel microchip that has been developed to combine multiple steps of the crystallographic pipeline from crystallization to diffraction data collection on a single device to streamline the entire process. The system has been designed for crystallization condition screening, visual crystal inspection, initial X-ray screening and data collection in a high-throughput fashion. X-ray diffraction data acquisition can be performed directly on-the-chip at room temperature using an in situ approach. The capabilities of the chip eliminate the necessity for manual crystal handling and cryoprotection of crystal samples, while allowing data collection from multiple crystals in the same drop. This technology would be especially beneficial for projects with large volumes of data, such as protein-complex studies and fragment-based screening. The platform employs hydrophilic and hydrophobic concentric ring surfaces on a miniature plate transparent to visible light and X-rays to create a well defined and stable microbatch crystallization environment. The results of crystallization and data-collection experiments demonstrate that high-quality well diffracting crystals can be grown and high-resolution diffraction data sets can be collected using this technology. Furthermore, the quality of a single-wavelength anomalous dispersion data set collected with the X-CHIP at room temperature was sufficient to generate interpretable electron-density maps. This technology is highly resource-efficient owing to the use of nanolitre-scale drop volumes. It does not require any modification for most in-house and synchrotron beamline systems and offers

Platform Constellations

DEFF Research Database (Denmark)

Staykova, Kalina Stefanova; Damsgaard, Jan

2016-01-01

This research paper presents an initial attempt to introduce and explain the emergence of new phenomenon, which we refer to as platform constellations. Functioning as highly modular systems, the platform constellations are collections of highly connected platforms which co-exist in parallel and a......’ acquisition and users’ engagement rates as well as unlock new sources of value creation and diversify revenue streams....

Construction and use of a microfluidic dissection platform for long-term imaging of cellular processes in budding yeast.

Science.gov (United States)

Huberts, Daphne H E W; Sik Lee, Sung; Gonzáles, Javier; Janssens, Georges E; Vizcarra, Ima Avalos; Heinemann, Matthias

2013-06-01

This protocol describes the production and operation of a microfluidic dissection platform for long-term, high-resolution imaging of budding yeast cells. At the core of this platform is an array of micropads that trap yeast cells in a single focal plane. Newly formed daughter cells are subsequently washed away by a continuous flow of fresh culture medium. In a typical experiment, 50-100 cells can be tracked during their entire replicative lifespan. Apart from aging-related research, the microfluidic platform can also be a valuable tool for other studies requiring the monitoring of single cells over time. Here we provide step-by-step instructions on how to fabricate the silicon wafer mold, how to produce and operate the microfluidic device and how to analyze the obtained data. Production of the microfluidic dissection platform and setting up an aging experiment takes ~7 h.

Simulating next-generation Cyber-physical computing platforms

OpenAIRE

Burgio, Paolo; Álvarez Martínez, Carlos; Ayguadé Parra, Eduard; Filgueras Izquierdo, Antonio; Jiménez González, Daniel; Martorell Bofill, Xavier; Navarro, Nacho; Giorgi, Roberto

2015-01-01

In specific domains, such as cyber-physical systems, platforms are quickly evolving to include multiple (many-) cores and programmable logic in a single system-on-chip, while includ- ing interfaces to commodity sensors/actuators. Programmable Logic (e.g., FPGA) allows for greater flexibility and dependability. However, the task of extracting the performance/watt potentia l of heterogeneous many-cores is often demanded at the application level, and this h...

Embedded Linux platform for data acquisition systems

International Nuclear Information System (INIS)

Patel, Jigneshkumar J.; Reddy, Nagaraj; Kumari, Praveena; Rajpal, Rachana; Pujara, Harshad; Jha, R.; Kalappurakkal, Praveen

2014-01-01

Highlights: • The design and the development of data acquisition system on FPGA based reconfigurable hardware platform. • Embedded Linux configuration and compilation for FPGA based systems. • Hardware logic IP core and its Linux device driver development for the external peripheral to interface it with the FPGA based system. - Abstract: This scalable hardware–software system is designed and developed to explore the emerging open standards for data acquisition requirement of Tokamak experiments. To address the future need for a scalable data acquisition and control system for fusion experiments, we have explored the capability of software platform using Open Source Embedded Linux Operating System on a programmable hardware platform such as FPGA. The idea was to identify the platform which can be customizable, flexible and scalable to support the data acquisition system requirements. To do this, we have selected FPGA based reconfigurable and scalable hardware platform to design the system with Embedded Linux based operating system for flexibility in software development and Gigabit Ethernet interface for high speed data transactions. The proposed hardware–software platform using FPGA and Embedded Linux OS offers a single chip solution with processor, peripherals such ADC interface controller, Gigabit Ethernet controller, memory controller amongst other peripherals. The Embedded Linux platform for data acquisition is implemented and tested on a Virtex-5 FXT FPGA ML507 which has PowerPC 440 (PPC440) [2] hard block on FPGA. For this work, we have used the Linux Kernel version 2.6.34 with BSP support for the ML507 platform. It is downloaded from the Xilinx [1] GIT server. Cross-compiler tool chain is created using the Buildroot scripts. The Linux Kernel and Root File System are configured and compiled using the cross-tools to support the hardware platform. The Analog to Digital Converter (ADC) IO module is designed and interfaced with the ML507 through Xilinx

Embedded Linux platform for data acquisition systems

Energy Technology Data Exchange (ETDEWEB)

Patel, Jigneshkumar J., E-mail: jjp@ipr.res.in [Institute for Plasma Research, Gandhinagar, Gujarat (India); Reddy, Nagaraj, E-mail: nagaraj.reddy@coreel.com [Sandeepani School of Embedded System Design, Bangalore, Karnataka (India); Kumari, Praveena, E-mail: praveena@ipr.res.in [Institute for Plasma Research, Gandhinagar, Gujarat (India); Rajpal, Rachana, E-mail: rachana@ipr.res.in [Institute for Plasma Research, Gandhinagar, Gujarat (India); Pujara, Harshad, E-mail: pujara@ipr.res.in [Institute for Plasma Research, Gandhinagar, Gujarat (India); Jha, R., E-mail: rjha@ipr.res.in [Institute for Plasma Research, Gandhinagar, Gujarat (India); Kalappurakkal, Praveen, E-mail: praveen.k@coreel.com [Sandeepani School of Embedded System Design, Bangalore, Karnataka (India)

2014-05-15

Highlights: • The design and the development of data acquisition system on FPGA based reconfigurable hardware platform. • Embedded Linux configuration and compilation for FPGA based systems. • Hardware logic IP core and its Linux device driver development for the external peripheral to interface it with the FPGA based system. - Abstract: This scalable hardware–software system is designed and developed to explore the emerging open standards for data acquisition requirement of Tokamak experiments. To address the future need for a scalable data acquisition and control system for fusion experiments, we have explored the capability of software platform using Open Source Embedded Linux Operating System on a programmable hardware platform such as FPGA. The idea was to identify the platform which can be customizable, flexible and scalable to support the data acquisition system requirements. To do this, we have selected FPGA based reconfigurable and scalable hardware platform to design the system with Embedded Linux based operating system for flexibility in software development and Gigabit Ethernet interface for high speed data transactions. The proposed hardware–software platform using FPGA and Embedded Linux OS offers a single chip solution with processor, peripherals such ADC interface controller, Gigabit Ethernet controller, memory controller amongst other peripherals. The Embedded Linux platform for data acquisition is implemented and tested on a Virtex-5 FXT FPGA ML507 which has PowerPC 440 (PPC440) [2] hard block on FPGA. For this work, we have used the Linux Kernel version 2.6.34 with BSP support for the ML507 platform. It is downloaded from the Xilinx [1] GIT server. Cross-compiler tool chain is created using the Buildroot scripts. The Linux Kernel and Root File System are configured and compiled using the cross-tools to support the hardware platform. The Analog to Digital Converter (ADC) IO module is designed and interfaced with the ML507 through Xilinx

Continuous Platform Development

DEFF Research Database (Denmark)

Nielsen, Ole Fiil

low risks and investments but also with relatively fuzzy results. When looking for new platform projects, it is important to make sure that the company and market is ready for the introduction of platforms, and to make sure that people from marketing and sales, product development, and downstream......, but continuous product family evolution challenges this strategy. The concept of continuous platform development is based on the fact that platform development should not be a one-time experience but rather an ongoing process of developing new platforms and updating existing ones, so that product family...

Genomics Portals: integrative web-platform for mining genomics data.

Science.gov (United States)

Shinde, Kaustubh; Phatak, Mukta; Johannes, Freudenberg M; Chen, Jing; Li, Qian; Vineet, Joshi K; Hu, Zhen; Ghosh, Krishnendu; Meller, Jaroslaw; Medvedovic, Mario

2010-01-13

A large amount of experimental data generated by modern high-throughput technologies is available through various public repositories. Our knowledge about molecular interaction networks, functional biological pathways and transcriptional regulatory modules is rapidly expanding, and is being organized in lists of functionally related genes. Jointly, these two sources of information hold a tremendous potential for gaining new insights into functioning of living systems. Genomics Portals platform integrates access to an extensive knowledge base and a large database of human, mouse, and rat genomics data with basic analytical visualization tools. It provides the context for analyzing and interpreting new experimental data and the tool for effective mining of a large number of publicly available genomics datasets stored in the back-end databases. The uniqueness of this platform lies in the volume and the diversity of genomics data that can be accessed and analyzed (gene expression, ChIP-chip, ChIP-seq, epigenomics, computationally predicted binding sites, etc), and the integration with an extensive knowledge base that can be used in such analysis. The integrated access to primary genomics data, functional knowledge and analytical tools makes Genomics Portals platform a unique tool for interpreting results of new genomics experiments and for mining the vast amount of data stored in the Genomics Portals backend databases. Genomics Portals can be accessed and used freely at http://GenomicsPortals.org.

Coupling single emitters to quantum plasmonic circuits

DEFF Research Database (Denmark)

Huck, Alexander; Andersen, Ulrik Lund

2016-01-01

In recent years, the controlled coupling of single-photon emitters to propagating surface plasmons has been intensely studied, which is fueled by the prospect of a giant photonic nonlinearity on a nanoscaled platform. In this article, we will review the recent progress on coupling single emitters...

Single-mode glass waveguide technology for optical interchip communication on board level

Science.gov (United States)

Brusberg, Lars; Neitz, Marcel; Schröder, Henning

2012-01-01

The large bandwidth demand in long-distance telecom networks lead to single-mode fiber interconnects as result of low dispersion, low loss and dense wavelength multiplexing possibilities. In contrast, multi-mode interconnects are suitable for much shorter lengths up to 300 meters and are promising for optical links between racks and on board level. Active optical cables based on multi-mode fiber links are at the market and research in multi-mode waveguide integration on board level is still going on. Compared to multi-mode, a single-mode waveguide has much more integration potential because of core diameters of around 20% of a multi-mode waveguide by a much larger bandwidth. But light coupling in single-mode waveguides is much more challenging because of lower coupling tolerances. Together with the silicon photonics technology, a single-mode waveguide technology on board-level will be the straight forward development goal for chip-to-chip optical interconnects integration. Such a hybrid packaging platform providing 3D optical single-mode links bridges the gap between novel photonic integrated circuits and the glass fiber based long-distance telecom networks. Following we introduce our 3D photonic packaging approach based on thin glass substrates with planar integrated optical single-mode waveguides for fiber-to-chip and chip-to-chip interconnects. This novel packaging approach merges micro-system packaging and glass integrated optics. It consists of a thin glass substrate with planar integrated singlemode waveguide circuits, optical mirrors and lenses providing an integration platform for photonic IC assembly and optical fiber interconnect. Thin glass is commercially available in panel and wafer formats and characterizes excellent optical and high-frequency properties. That makes it perfect for microsystem packaging. The paper presents recent results in single-mode waveguide technology on wafer level and waveguide characterization. Furthermore the integration in a

Graph processing platforms at scale: practices and experiences

Energy Technology Data Exchange (ETDEWEB)

Lim, Seung-Hwan [ORNL; Lee, Sangkeun (Matt) [ORNL; Brown, Tyler C [ORNL; Sukumar, Sreenivas R [ORNL; Ganesh, Gautam [ORNL

2015-01-01

Graph analysis unveils hidden associations of data in many phenomena and artifacts, such as road network, social networks, genomic information, and scientific collaboration. Unfortunately, a wide diversity in the characteristics of graphs and graph operations make it challenging to find a right combination of tools and implementation of algorithms to discover desired knowledge from the target data set. This study presents an extensive empirical study of three representative graph processing platforms: Pegasus, GraphX, and Urika. Each system represents a combination of options in data model, processing paradigm, and infrastructure. We benchmarked each platform using three popular graph operations, degree distribution, connected components, and PageRank over a variety of real-world graphs. Our experiments show that each graph processing platform shows different strength, depending the type of graph operations. While Urika performs the best in non-iterative operations like degree distribution, GraphX outputforms iterative operations like connected components and PageRank. In addition, we discuss challenges to optimize the performance of each platform over large scale real world graphs.

A Short Survey on the State of the Art in Architectures and Platforms for Large Scale Data Analysis and Knowledge Discovery from Data

Energy Technology Data Exchange (ETDEWEB)

Begoli, Edmon [ORNL

2012-01-01

Intended as a survey for practicing architects and researchers seeking an overview of the state-of-the-art architectures for data analysis, this paper provides an overview of the emerg- ing data management and analytic platforms including par- allel databases, Hadoop-based systems, High Performance Computing (HPC) platforms and platforms popularly re- ferred to as NoSQL platforms. Platforms are presented based on their relevance, analysis they support and the data organization model they support.

Multi-octave spectral beam combiner on ultra-broadband photonic integrated circuit platform.

Science.gov (United States)

Stanton, Eric J; Heck, Martijn J R; Bovington, Jock; Spott, Alexander; Bowers, John E

2015-05-04

We present the design of a novel platform that is able to combine optical frequency bands spanning 4.2 octaves from ultraviolet to mid-wave infrared into a single, low M2 output waveguide. We present the design and realization of a key component in this platform that combines the wavelength bands of 350 nm - 1500 nm and 1500 nm - 6500 nm with demonstrated efficiency greater than 90% in near-infrared and mid-wave infrared. The multi-octave spectral beam combiner concept is realized using an integrated platform with silicon nitride waveguides and silicon waveguides. Simulated bandwidth is shown to be over four octaves, and measured bandwidth is shown over two octaves, limited by the availability of sources.

High-recovery visual identification and single-cell retrieval of circulating tumor cells for genomic analysis using a dual-technology platform integrated with automated immunofluorescence staining

International Nuclear Information System (INIS)

Campton, Daniel E; Ramirez, Arturo B; Nordberg, Joshua J; Drovetto, Nick; Clein, Alisa C; Varshavskaya, Paulina; Friemel, Barry H; Quarre, Steve; Breman, Amy; Dorschner, Michael; Blau, Sibel; Blau, C Anthony; Sabath, Daniel E; Stilwell, Jackie L; Kaldjian, Eric P

2015-01-01

Circulating tumor cells (CTCs) are malignant cells that have migrated from solid cancers into the blood, where they are typically present in rare numbers. There is great interest in using CTCs to monitor response to therapies, to identify clinically actionable biomarkers, and to provide a non-invasive window on the molecular state of a tumor. Here we characterize the performance of the AccuCyte® – CyteFinder® system, a comprehensive, reproducible and highly sensitive platform for collecting, identifying and retrieving individual CTCs from microscopic slides for molecular analysis after automated immunofluorescence staining for epithelial markers. All experiments employed a density-based cell separation apparatus (AccuCyte) to separate nucleated cells from the blood and transfer them to microscopic slides. After staining, the slides were imaged using a digital scanning microscope (CyteFinder). Precisely counted model CTCs (mCTCs) from four cancer cell lines were spiked into whole blood to determine recovery rates. Individual mCTCs were removed from slides using a single-cell retrieval device (CytePicker™) for whole genome amplification and subsequent analysis by PCR and Sanger sequencing, whole exome sequencing, or array-based comparative genomic hybridization. Clinical CTCs were evaluated in blood samples from patients with different cancers in comparison with the CellSearch® system. AccuCyte – CyteFinder presented high-resolution images that allowed identification of mCTCs by morphologic and phenotypic features. Spike-in mCTC recoveries were between 90 and 91%. More than 80% of single-digit spike-in mCTCs were identified and even a single cell in 7.5 mL could be found. Analysis of single SKBR3 mCTCs identified presence of a known TP53 mutation by both PCR and whole exome sequencing, and confirmed the reported karyotype of this cell line. Patient sample CTC counts matched or exceeded CellSearch CTC counts in a small feasibility cohort. The AccuCyte �

Towards Polarization Diversity on the SOI Platform With Simple Fabrication Process

DEFF Research Database (Denmark)

Ding, Yunhong; Liu, Liu; Peucheret, Christophe

2011-01-01

We present a polarization diversity circuit built on the silicon-on-insulator (SOI) platform, which can be fabricated by a simple process. The polarization diversity is based on two identical air-clad asymmetrical directional couplers, which simultaneously play the roles of polarization splitter...... and rotator. A silicon polarization diversity circuit with a single microring resonator is fabricated on the SOI platform. Only ${1-dB polarization-dependent loss is demonstrated. A significant improvement of the polarization dependence is obtained for 20-Gb/s nonreturn-to-zero differential phase-shift keying...

A multi-landing pad DNA integration platform for mammalian cell engineering

Science.gov (United States)

Gaidukov, Leonid; Wroblewska, Liliana; Teague, Brian; Nelson, Tom; Zhang, Xin; Liu, Yan; Jagtap, Kalpana; Mamo, Selamawit; Tseng, Wen Allen; Lowe, Alexis; Das, Jishnu; Bandara, Kalpanie; Baijuraj, Swetha; Summers, Nevin M; Zhang, Lin; Weiss, Ron

2018-01-01

Abstract Engineering mammalian cell lines that stably express many transgenes requires the precise insertion of large amounts of heterologous DNA into well-characterized genomic loci, but current methods are limited. To facilitate reliable large-scale engineering of CHO cells, we identified 21 novel genomic sites that supported stable long-term expression of transgenes, and then constructed cell lines containing one, two or three ‘landing pad’ recombination sites at selected loci. By using a highly efficient BxB1 recombinase along with different selection markers at each site, we directed recombinase-mediated insertion of heterologous DNA to selected sites, including targeting all three with a single transfection. We used this method to controllably integrate up to nine copies of a monoclonal antibody, representing about 100 kb of heterologous DNA in 21 transcriptional units. Because the integration was targeted to pre-validated loci, recombinant protein expression remained stable for weeks and additional copies of the antibody cassette in the integrated payload resulted in a linear increase in antibody expression. Overall, this multi-copy site-specific integration platform allows for controllable and reproducible insertion of large amounts of DNA into stable genomic sites, which has broad applications for mammalian synthetic biology, recombinant protein production and biomanufacturing. PMID:29617873

A Survey of Open Source Products for Building a SIP Communication Platform

Directory of Open Access Journals (Sweden)

Pavel Segec

2011-01-01

Full Text Available The Session Initiation Protocol (SIP is a multimedia signalling protocol that has evolved into a widely adopted communication standard. The integration of SIP into existing IP networks has fostered IP networks becoming a convergence platform for both real-time and non-real-time multimedia communications. This converged platform integrates data, voice, video, presence, messaging, and conference services into a single network that offers new communication experiences for users. The open source community has contributed to SIP adoption through the development of open source software for both SIP clients and servers. In this paper, we provide a survey on open SIP systems that can be built using publically available software. We identify SIP features for service development and programming, services and applications of a SIP-converged platform, and the most important technologies supporting SIP functionalities. We propose an advanced converged IP communication platform that uses SIP for service delivery. The platform supports audio and video calls, along with media services such as audio conferences, voicemail, presence, and instant messaging. Using SIP Application Programming Interfaces (APIs, the platform allows the deployment of advanced integrated services. The platform is implemented with open source software. Architecture components run on standardized hardware with no need for special purpose investments.

3D-SURFER 2.0: web platform for real-time search and characterization of protein surfaces.

Science.gov (United States)

Xiong, Yi; Esquivel-Rodriguez, Juan; Sael, Lee; Kihara, Daisuke

2014-01-01

The increasing number of uncharacterized protein structures necessitates the development of computational approaches for function annotation using the protein tertiary structures. Protein structure database search is the basis of any structure-based functional elucidation of proteins. 3D-SURFER is a web platform for real-time protein surface comparison of a given protein structure against the entire PDB using 3D Zernike descriptors. It can smoothly navigate the protein structure space in real-time from one query structure to another. A major new feature of Release 2.0 is the ability to compare the protein surface of a single chain, a single domain, or a single complex against databases of protein chains, domains, complexes, or a combination of all three in the latest PDB. Additionally, two types of protein structures can now be compared: all-atom-surface and backbone-atom-surface. The server can also accept a batch job for a large number of database searches. Pockets in protein surfaces can be identified by VisGrid and LIGSITE (csc) . The server is available at http://kiharalab.org/3d-surfer/.

Information security threats in web-portals on the open journal systems platform

Directory of Open Access Journals (Sweden)

Anton A. Abramov

2018-05-01

Full Text Available This article addresses the problem of security threats while working with web portals built on the Open Journal Systems platform. The Open Journal Systems (OJS platform was originally developed as part of the Public Knowledge Project and it is one of the most popular open-source platforms for web journals today. Based on the data available in the Public Knowledge Project, there were more than 10,000 active journals using the open journal systems platform by the end of 2016. A migration of a journal to such advanced and complex platform helps to handle the entire workflow over a single web portal. Therefore it is an important move and only peer-reviewed journals that are part of Russian and Worldwide citation systems go for it. At the same time the problem of keeping privacy for a manuscript before it is published is very important for these journals and for authors who submit it to the journal. The paper describes the most common threats for the web portals on the OJS platform as well as a particular model of the security threats, and suggests the measures that could help to neutralize these threats.

Angularly-resolved elastic scatter from single particles collected over a large solid angle and with high resolution

International Nuclear Information System (INIS)

Aptowicz, Kevin B; Chang, Richard K

2005-01-01

Elastic light scattering from a single non-spherical particle of various morphologies has been measured simultaneously with a large angular range (90 deg. < θ < 165 deg. and 0 deg. < φ < 360 deg.) and with high angular resolution (1024 pixels in θ and 512 pixels in φ). Because the single-shot laser pulse is short (pulse duration of 70 ns), the tumbling and flowing particle can be treated as frozen in space. The large angle two-dimensional angular optical scattering (hereafter referred to as LA TAOS) intensity pattern, I(θ,φ), has been measured for a variety of particle morphology, such as the following: (1) single polystyrene latex (PSL) sphere; (2) cluster of PSL spheres; (3) single Bacillus subtilis (BG) spore; (4) cluster of BG spores; (5) dried aggregates of bio-aerosols as well as background clutter aerosols. All these measurements were made using the second harmonic of a Nd:YAG laser (0.532 μm). Islands structures in the LA TAOS patterns seem to be the prominent feature. Efforts are being made to extract metrics from these islands and compare them to theoretical results based on the T-matrix method

NiftyPET: a High-throughput Software Platform for High Quantitative Accuracy and Precision PET Imaging and Analysis.

Science.gov (United States)

Markiewicz, Pawel J; Ehrhardt, Matthias J; Erlandsson, Kjell; Noonan, Philip J; Barnes, Anna; Schott, Jonathan M; Atkinson, David; Arridge, Simon R; Hutton, Brian F; Ourselin, Sebastien

2018-01-01

We present a standalone, scalable and high-throughput software platform for PET image reconstruction and analysis. We focus on high fidelity modelling of the acquisition processes to provide high accuracy and precision quantitative imaging, especially for large axial field of view scanners. All the core routines are implemented using parallel computing available from within the Python package NiftyPET, enabling easy access, manipulation and visualisation of data at any processing stage. The pipeline of the platform starts from MR and raw PET input data and is divided into the following processing stages: (1) list-mode data processing; (2) accurate attenuation coefficient map generation; (3) detector normalisation; (4) exact forward and back projection between sinogram and image space; (5) estimation of reduced-variance random events; (6) high accuracy fully 3D estimation of scatter events; (7) voxel-based partial volume correction; (8) region- and voxel-level image analysis. We demonstrate the advantages of this platform using an amyloid brain scan where all the processing is executed from a single and uniform computational environment in Python. The high accuracy acquisition modelling is achieved through span-1 (no axial compression) ray tracing for true, random and scatter events. Furthermore, the platform offers uncertainty estimation of any image derived statistic to facilitate robust tracking of subtle physiological changes in longitudinal studies. The platform also supports the development of new reconstruction and analysis algorithms through restricting the axial field of view to any set of rings covering a region of interest and thus performing fully 3D reconstruction and corrections using real data significantly faster. All the software is available as open source with the accompanying wiki-page and test data.

Communicating climate change adaptation information using web-based platforms

Directory of Open Access Journals (Sweden)

E. Karali

2017-07-01

Full Text Available To facilitate progress in climate change adaptation policy and practice, it is important not only to ensure the production of accurate, comprehensive and relevant information, but also the easy, timely and affordable access to it. This can contribute to better-informed decisions and improve the design and implementation of adaptation policies and other relevant initiatives. Web-based platforms can play an important role in communicating and distributing data, information and knowledge that become constantly available, reaching out to a large group of potential users. Indeed in the last decade there has been an extensive increase in the number of platforms developed for this purpose in many fields including climate change adaptation. This short paper concentrates on the web-based adaptation platforms developed in Europe. It provides an overview of the recently emerged landscape, examines the basic characteristics of a set of platforms that operate at national, transnational and European level, and discusses some of the key challenges related to their development, maintenance and overall management. Findings presented in this short paper are discussed in greater detailed in the Technical Report of the European Environment Agency Overview of climate change adaptation platforms in Europe.

Communicating climate change adaptation information using web-based platforms

Science.gov (United States)

Karali, Eleni; Mattern, Kati

2017-07-01

To facilitate progress in climate change adaptation policy and practice, it is important not only to ensure the production of accurate, comprehensive and relevant information, but also the easy, timely and affordable access to it. This can contribute to better-informed decisions and improve the design and implementation of adaptation policies and other relevant initiatives. Web-based platforms can play an important role in communicating and distributing data, information and knowledge that become constantly available, reaching out to a large group of potential users. Indeed in the last decade there has been an extensive increase in the number of platforms developed for this purpose in many fields including climate change adaptation. This short paper concentrates on the web-based adaptation platforms developed in Europe. It provides an overview of the recently emerged landscape, examines the basic characteristics of a set of platforms that operate at national, transnational and European level, and discusses some of the key challenges related to their development, maintenance and overall management. Findings presented in this short paper are discussed in greater detailed in the Technical Report of the European Environment Agency Overview of climate change adaptation platforms in Europe.

A Dual-Mode UWB Wireless Platform with Random Pulse Length Detection for Remote Patient Monitoring

DEFF Research Database (Denmark)

Reyes, Carlos; Bisbe, Sergi; Shen, Ming

2013-01-01

on a single hardware platform, but it is capable of both monitoring and data transmission. This is achieved by employing a new random pulse length detection method that allows data transmission by using a modulated monitoring signal. To prove the proposed concept a test system has been built, using commercial......This paper presents a dual-mode ultra-wideband platform for wireless Remote Patient Monitoring (RPM). Existing RPM solutions are typically based on two different hardware platforms; one responsible for medical-data monitoring and one to handle data transmission. The proposed RPM topology is based...

Carbonate-platform response to the Toarcian Oceanic Anoxic Event in the southern hemisphere: Implications for climatic change and biotic platform demise

Science.gov (United States)

Han, Zhong; Hu, Xiumian; Kemp, David B.; Li, Juan

2018-05-01

The Toarcian Oceanic Anoxic Event (T-OAE, ∼183 Ma) was a profound short-term environmental perturbation associated with the large-scale release of 13C-depleted carbon into the global ocean-atmosphere system, which resulted in a significant negative carbon-isotope excursion (CIE). The general lack of characteristic T-OAE records outside of the northern hemisphere means that the precise environmental effects and significance of this event are uncertain. Many biotic carbonate platforms of the northern hemisphere western Tethys drowned or shifted to non-skeletal platforms during the early Toarcian. However, southern hemisphere records of Toarcian carbonate platforms are rare, and thus the extent and significance of biotic platform demise during the T-OAE is unclear. Here we present high-resolution geochemical and sedimentological data across two Pliensbachian-Toarcian shallow-water carbonate-platform sections exposed in the Tibetan Himalaya. These sections were located paleogeographically on the open southeastern tropical Tethyan margin in the southern hemisphere. The T-OAE in the Tibetan Himalaya is marked by a negative CIE in organic matter. Our sedimentological analysis of the two sections reveals an abundance of storm deposits within the T-OAE interval, which emphasizes a close link between warming and tropical storms during the T-OAE event, in line with evidence recently provided from western Tethyan sections of the northern hemisphere. In addition, our analysis also reveals extensive biotic carbonate-platform demise by drowning or changing to non-skeletal carbonates coincident with the onset of the CIE. Taken together, our results suggest that rapid and pervasive seawater warming in response to carbon release likely played a significant role in sudden biotic carbonate platform demise, and suppression/postponement of biotic platform re-development along the whole tropical/subtropical Tethyan margin.

Development of embedded real-time and high-speed vision platform

Science.gov (United States)

Ouyang, Zhenxing; Dong, Yimin; Yang, Hua

2015-12-01

Currently, high-speed vision platforms are widely used in many applications, such as robotics and automation industry. However, a personal computer (PC) whose over-large size is not suitable and applicable in compact systems is an indispensable component for human-computer interaction in traditional high-speed vision platforms. Therefore, this paper develops an embedded real-time and high-speed vision platform, ER-HVP Vision which is able to work completely out of PC. In this new platform, an embedded CPU-based board is designed as substitution for PC and a DSP and FPGA board is developed for implementing image parallel algorithms in FPGA and image sequential algorithms in DSP. Hence, the capability of ER-HVP Vision with size of 320mm x 250mm x 87mm can be presented in more compact condition. Experimental results are also given to indicate that the real-time detection and counting of the moving target at a frame rate of 200 fps at 512 x 512 pixels under the operation of this newly developed vision platform are feasible.

Comparison of two Next Generation sequencing platforms for full genome sequencing of Classical Swine Fever Virus

DEFF Research Database (Denmark)

Fahnøe, Ulrik; Pedersen, Anders Gorm; Höper, Dirk

2013-01-01

to the consensus sequence. Additionally, we got an average sequence depth for the genome of 4000 for the Iontorrent PGM and 400 for the FLX platform making the mapping suitable for single nucleotide variant (SNV) detection. The analysis revealed a single non-silent SNV A10665G leading to the amino acid change D......Next Generation Sequencing (NGS) is becoming more adopted into viral research and will be the preferred technology in the years to come. We have recently sequenced several strains of Classical Swine Fever Virus (CSFV) by NGS on both Genome Sequencer FLX (GS FLX) and Iontorrent PGM platforms...

arrayCGHbase: an analysis platform for comparative genomic hybridization microarrays

Directory of Open Access Journals (Sweden)

Moreau Yves

2005-05-01

Full Text Available Abstract Background The availability of the human genome sequence as well as the large number of physically accessible oligonucleotides, cDNA, and BAC clones across the entire genome has triggered and accelerated the use of several platforms for analysis of DNA copy number changes, amongst others microarray comparative genomic hybridization (arrayCGH. One of the challenges inherent to this new technology is the management and analysis of large numbers of data points generated in each individual experiment. Results We have developed arrayCGHbase, a comprehensive analysis platform for arrayCGH experiments consisting of a MIAME (Minimal Information About a Microarray Experiment supportive database using MySQL underlying a data mining web tool, to store, analyze, interpret, compare, and visualize arrayCGH results in a uniform and user-friendly format. Following its flexible design, arrayCGHbase is compatible with all existing and forthcoming arrayCGH platforms. Data can be exported in a multitude of formats, including BED files to map copy number information on the genome using the Ensembl or UCSC genome browser. Conclusion ArrayCGHbase is a web based and platform independent arrayCGH data analysis tool, that allows users to access the analysis suite through the internet or a local intranet after installation on a private server. ArrayCGHbase is available at http://medgen.ugent.be/arrayCGHbase/.

Long Read Alignment with Parallel MapReduce Cloud Platform

Directory of Open Access Journals (Sweden)

Ahmed Abdulhakim Al-Absi

2015-01-01

Full Text Available Genomic sequence alignment is an important technique to decode genome sequences in bioinformatics. Next-Generation Sequencing technologies produce genomic data of longer reads. Cloud platforms are adopted to address the problems arising from storage and analysis of large genomic data. Existing genes sequencing tools for cloud platforms predominantly consider short read gene sequences and adopt the Hadoop MapReduce framework for computation. However, serial execution of map and reduce phases is a problem in such systems. Therefore, in this paper, we introduce Burrows-Wheeler Aligner’s Smith-Waterman Alignment on Parallel MapReduce (BWASW-PMR cloud platform for long sequence alignment. The proposed cloud platform adopts a widely accepted and accurate BWA-SW algorithm for long sequence alignment. A custom MapReduce platform is developed to overcome the drawbacks of the Hadoop framework. A parallel execution strategy of the MapReduce phases and optimization of Smith-Waterman algorithm are considered. Performance evaluation results exhibit an average speed-up of 6.7 considering BWASW-PMR compared with the state-of-the-art Bwasw-Cloud. An average reduction of 30% in the map phase makespan is reported across all experiments comparing BWASW-PMR with Bwasw-Cloud. Optimization of Smith-Waterman results in reducing the execution time by 91.8%. The experimental study proves the efficiency of BWASW-PMR for aligning long genomic sequences on cloud platforms.

Long Read Alignment with Parallel MapReduce Cloud Platform

Science.gov (United States)

Al-Absi, Ahmed Abdulhakim; Kang, Dae-Ki

2015-01-01

Genomic sequence alignment is an important technique to decode genome sequences in bioinformatics. Next-Generation Sequencing technologies produce genomic data of longer reads. Cloud platforms are adopted to address the problems arising from storage and analysis of large genomic data. Existing genes sequencing tools for cloud platforms predominantly consider short read gene sequences and adopt the Hadoop MapReduce framework for computation. However, serial execution of map and reduce phases is a problem in such systems. Therefore, in this paper, we introduce Burrows-Wheeler Aligner's Smith-Waterman Alignment on Parallel MapReduce (BWASW-PMR) cloud platform for long sequence alignment. The proposed cloud platform adopts a widely accepted and accurate BWA-SW algorithm for long sequence alignment. A custom MapReduce platform is developed to overcome the drawbacks of the Hadoop framework. A parallel execution strategy of the MapReduce phases and optimization of Smith-Waterman algorithm are considered. Performance evaluation results exhibit an average speed-up of 6.7 considering BWASW-PMR compared with the state-of-the-art Bwasw-Cloud. An average reduction of 30% in the map phase makespan is reported across all experiments comparing BWASW-PMR with Bwasw-Cloud. Optimization of Smith-Waterman results in reducing the execution time by 91.8%. The experimental study proves the efficiency of BWASW-PMR for aligning long genomic sequences on cloud platforms. PMID:26839887

Genomics Portals: integrative web-platform for mining genomics data

Directory of Open Access Journals (Sweden)

Ghosh Krishnendu

2010-01-01

Full Text Available Abstract Background A large amount of experimental data generated by modern high-throughput technologies is available through various public repositories. Our knowledge about molecular interaction networks, functional biological pathways and transcriptional regulatory modules is rapidly expanding, and is being organized in lists of functionally related genes. Jointly, these two sources of information hold a tremendous potential for gaining new insights into functioning of living systems. Results Genomics Portals platform integrates access to an extensive knowledge base and a large database of human, mouse, and rat genomics data with basic analytical visualization tools. It provides the context for analyzing and interpreting new experimental data and the tool for effective mining of a large number of publicly available genomics datasets stored in the back-end databases. The uniqueness of this platform lies in the volume and the diversity of genomics data that can be accessed and analyzed (gene expression, ChIP-chip, ChIP-seq, epigenomics, computationally predicted binding sites, etc, and the integration with an extensive knowledge base that can be used in such analysis. Conclusion The integrated access to primary genomics data, functional knowledge and analytical tools makes Genomics Portals platform a unique tool for interpreting results of new genomics experiments and for mining the vast amount of data stored in the Genomics Portals backend databases. Genomics Portals can be accessed and used freely at http://GenomicsPortals.org.

Mobile platform security

CERN Document Server

Asokan, N; Dmitrienko, Alexandra

2013-01-01

Recently, mobile security has garnered considerable interest in both the research community and industry due to the popularity of smartphones. The current smartphone platforms are open systems that allow application development, also for malicious parties. To protect the mobile device, its user, and other mobile ecosystem stakeholders such as network operators, application execution is controlled by a platform security architecture. This book explores how such mobile platform security architectures work. We present a generic model for mobile platform security architectures: the model illustrat

Delft-FEWS:A Decision Making Platform to Intergrate Data, Model, Algorithm for Large-Scale River Basin Water Management

Science.gov (United States)

Yang, T.; Welles, E.

2017-12-01

In this paper, we introduce a flood forecasting and decision making platform, named Delft-FEWS, which has been developed over years at the Delft Hydraulics and now at Deltares. The philosophy of Delft-FEWS is to provide water managers and operators with an open shell tool, which allows the integratation of a variety of hydrological, hydraulics, river routing, and reservoir models with hydrometerological forecasts data. Delft-FEWS serves as an powerful tool for both basin-scale and national-scale water resources management. The essential novelty of Delft-FEWS is to change the flood forecasting and water resources management from a single model or agency centric paradigm to a intergrated framework, in which different model, data, algorithm and stakeholders are strongly linked together. The paper will start with the challenges in water resources managment, and the concept and philosophy of Delft-FEWS. Then, the details of data handling and linkages of Delft-FEWS with different hydrological, hydraulic, and reservoir models, etc. Last, several cases studies and applications of Delft-FEWS will be demonstrated, including the National Weather Service and the Bonneville Power Administration in USA, and a national application in the water board in the Netherland.

Using a grid platform for solving large sparse linear systems over GF(2)

OpenAIRE

Kleinjung , Thorsten; Nussbaum , Lucas; Thomé , Emmanuel

2010-01-01

International audience; In Fall 2009, the final step of the factorization of rsa768 was carried out on several clusters of the Grid'5000 platform, leading to a new record in integer factorization. This step involves solving a huge sparse linear system defined over the binary field GF(2). This article aims at describing the algorithm used, the difficulties encountered, and the methodology which led to success. In particular, we illustrate how our use of the block Wiedemann algorithm led to a m...

Towards a Scalable and Adaptive Application Support Platform for Large-Scale Distributed E-Sciences in High-Performance Network Environments

Energy Technology Data Exchange (ETDEWEB)

Wu, Chase Qishi [New Jersey Inst. of Technology, Newark, NJ (United States); Univ. of Memphis, TN (United States); Zhu, Michelle Mengxia [Southern Illinois Univ., Carbondale, IL (United States)

2016-06-06

The advent of large-scale collaborative scientific applications has demonstrated the potential for broad scientific communities to pool globally distributed resources to produce unprecedented data acquisition, movement, and analysis. System resources including supercomputers, data repositories, computing facilities, network infrastructures, storage systems, and display devices have been increasingly deployed at national laboratories and academic institutes. These resources are typically shared by large communities of users over Internet or dedicated networks and hence exhibit an inherent dynamic nature in their availability, accessibility, capacity, and stability. Scientific applications using either experimental facilities or computation-based simulations with various physical, chemical, climatic, and biological models feature diverse scientific workflows as simple as linear pipelines or as complex as a directed acyclic graphs, which must be executed and supported over wide-area networks with massively distributed resources. Application users oftentimes need to manually configure their computing tasks over networks in an ad hoc manner, hence significantly limiting the productivity of scientists and constraining the utilization of resources. The success of these large-scale distributed applications requires a highly adaptive and massively scalable workflow platform that provides automated and optimized computing and networking services. This project is to design and develop a generic Scientific Workflow Automation and Management Platform (SWAMP), which contains a web-based user interface specially tailored for a target application, a set of user libraries, and several easy-to-use computing and networking toolkits for application scientists to conveniently assemble, execute, monitor, and control complex computing workflows in heterogeneous high-performance network environments. SWAMP will enable the automation and management of the entire process of scientific

Nanofilter platform based on functionalized carbon nanotubes for adsorption and elimination of Acrolein, a toxicant in cigarette smoke

Science.gov (United States)

Yoosefian, Mehdi; Pakpour, Atef; Etminan, Nazanin

2018-06-01

This paper discusses the use of carboxylated single-walled carbon nanotube as a general nanofilter platform for the removal of acrolein carcinogen from cigarette smoke. The analyses carried out in the detailed study of the electronic and structural effects of the adsorption of acrolein onto COOH loaded on single-walled carbon nanotube under the density functional theory framework. The results of Bader theory of atoms in molecules, natural bond orbital, molecular potential electron surface and density of state confirm the potential application of the suggested nanofilter platform.

Versatile and Efficient Targeting Using a Single Nanoparticulate Platform: Application to Cancer and Alzheimer's Disease

NARCIS (Netherlands)

Le Droumaguet, Benjamin; Nicolas, Julien; Brambilla, Davide; Mura, Simona; Maksimenko, Andrei; de Kimpe, Line; Salvati, Elisa; Zona, Cristiano; Airoldi, Cristina; Canovi, Mara; Gobbi, Marco; Magali, Noiray; La Ferla, Barbara; Nicotra, Francesco; Scheper, Wiep; Flores, Orfeu; Masserini, Massimo; Andrieux, Karine; Couvreur, Patrick

2012-01-01

A versatile and efficient functionalization strategy for polymeric nanoparticles (NPs) has been reported and successfully applied to PEGylated, biodegradable poly(alkyl cyanoacrylate) (PACA) nanocarriers. The relevance of this platform was demonstrated in both the fields of cancer and Alzheimer's

National symposium and workshop on optical platforms, Huntsville, AL, June 12-14, 1984, Proceedings

International Nuclear Information System (INIS)

Wyman, C.L.

1984-01-01

The conference is concerned with space-based observatories (including future systems), interplanetary observation platforms, Space Station optical utilization, orbital earth viewing systems, and Spacelab's use as an observations platform. Attention is given to the pointing system for the space telescope, the Advanced X-ray Astrophysics Facility, the conceptual definition of the Space IR Telescope Facility's spacecraft, satellite servicing in light of the Solar Maximum Repair Mission, a large deployable reflector telescope concept, moon-based astronomical observatories, Space Station-based remote sensing of the earth, optical systems is geosynchronous orbit, multispectral linear array detector technology, Spacelab-based space plasma and materials processing investigations, and space construction technology for large space observatories. Also discussed are military optical platforms for sea, land and air stationing

A microwell cell culture platform for the aggregation of pancreatic β-cells.

Science.gov (United States)

Bernard, Abigail B; Lin, Chien-Chi; Anseth, Kristi S

2012-08-01

Cell-cell contact between pancreatic β-cells is important for maintaining survival and normal insulin secretion. Various techniques have been developed to promote cell-cell contact between β-cells, but a simple yet robust method that affords precise control over three-dimensional (3D) β-cell cluster size has not been demonstrated. To address this need, we developed a poly(ethylene glycol) (PEG) hydrogel microwell platform using photolithography. This microwell cell-culture platform promotes the formation of 3D β-cell aggregates of defined sizes from 25 to 210 μm in diameter. Using this platform, mouse insulinoma 6 (MIN6) β-cells formed aggregates with cell-cell adherin junctions. These naturally formed cell aggregates with controllable sizes can be removed from the microwells for macroencapsulation, implantation, or other biological assays. When removed and subsequently encapsulated in PEG hydrogels, the aggregated cell clusters demonstrated improved cellular viability (>90%) over 7 days in culture, while the β-cells encapsulated as single cells maintained only 20% viability. Aggregated MIN6 cells also exhibited more than fourfold higher insulin secretion in response to a glucose challenge compared with encapsulated single β-cells. Further, the cell aggregates stained positively for E-cadherin, indicative of the formation of cell junctions. Using this hydrogel microwell cell-culture method, viable and functional β-cell aggregates of specific sizes were created, providing a platform from which other biologically relevant questions may be answered.

Single-photon source engineering using a Modal Method

DEFF Research Database (Denmark)

Gregersen, Niels

Solid-state sources of single indistinguishable photons are of great interest for quantum information applications. The semiconductor quantum dot embedded in a host material represents an attractive platform to realize such a single-photon source (SPS). A near-unity efficiency, defined as the num...... nanowire SPSs...

Adaptive Backstepping Controller Design for Leveling Control of an Underwater Platform Based on Joint Space

Directory of Open Access Journals (Sweden)

Zhi-Lin Zeng

2014-01-01

Full Text Available This paper focuses on high precision leveling control of an underwater heavy load platform, which is viewed as an underwater parallel robot on the basis of its work pattern. The kinematic of platform with deformation is analyzed and the dynamics model of joint space is established. An adaptive backstepping controller according to Lyapunov's function is proposed for leveling control of platform based on joint space. Furthermore, the “lowest point fixed angle error” leveling scheme called “chase” is chosen for leveling control of platform. The digital simulation and practical experiment of single joint space actuator are carried out, and the results show high precision servo control of joint space. On the basis of this, the platform leveling control simulation relies on the hardware-in-loop system. The results indicate that the proposed controller can effectively restrain the influence from system parameter uncertainties and external disturbance to realize high precision leveling control of the underwater platform.

Comparison Of Hybrid Sorting Algorithms Implemented On Different Parallel Hardware Platforms

Directory of Open Access Journals (Sweden)

Dominik Zurek

2013-01-01

Full Text Available Sorting is a common problem in computer science. There are lot of well-known sorting algorithms created for sequential execution on a single processor. Recently, hardware platforms enable to create wide parallel algorithms. We have standard processors consist of multiple cores and hardware accelerators like GPU. The graphic cards with their parallel architecture give new possibility to speed up many algorithms. In this paper we describe results of implementation of a few different sorting algorithms on GPU cards and multicore processors. Then hybrid algorithm will be presented which consists of parts executed on both platforms, standard CPU and GPU.

PLATFORM DEFORMATION PHASE CORRECTION FOR THE AMiBA-13 COPLANAR INTERFEROMETER

Energy Technology Data Exchange (ETDEWEB)

Liao, Yu-Wei; Lin, Kai-Yang; Huang, Yau-De; Ho, Paul T. P.; Chen, Ming-Tang; Locutus Huang, Chih-Wei; Koch, Patrick M.; Nishioka, Hiroaki; Umetsu, Keiichi; Han, Chih-Chiang; Li, Chao-Te; Martin-Cocher, Pierre; Oshiro, Peter [Institute of Astronomy and Astrophysics, Academia Sinica, P.O. Box 23-141, Taipei 10617, Taiwan (China); Proty Wu, Jiun-Huei; Cheng, Tai-An; Fu, Szu-Yuan; Wang, Fu-Cheng [Department of Physics, Institute of Astrophysics, and Center for Theoretical Sciences, National Taiwan University, Taipei 10617, Taiwan (China); Liu, Guo-Chin [Department of Physics, Tamkang University, 251-37 Tamsui, New Taipei City, Taiwan (China); Molnar, Sandor M. [Leung Center for Cosmology and Particle Astrophysics, National Taiwan University, Taipei 10617, Taiwan (China); Chang, Yu-Yen, E-mail: ywliao@asiaa.sinica.edu.tw, E-mail: jhpw@phys.ntu.edu.tw [Max-Planck-Institut fuer Astronomie, Koenigstuhl 17, D-69117 Heidelberg (Germany)

2013-05-20

We present a new way to solve the platform deformation problem of coplanar interferometers. The platform of a coplanar interferometer can be deformed due to driving forces and gravity. A deformed platform will induce extra components into the geometric delay of each baseline and change the phases of observed visibilities. The reconstructed images will also be diluted due to the errors of the phases. The platform deformations of The Yuan-Tseh Lee Array for Microwave Background Anisotropy (AMiBA) were modeled based on photogrammetry data with about 20 mount pointing positions. We then used the differential optical pointing error between two optical telescopes to fit the model parameters in the entire horizontal coordinate space. With the platform deformation model, we can predict the errors of the geometric phase delays due to platform deformation with a given azimuth and elevation of the targets and calibrators. After correcting the phases of the radio point sources in the AMiBA interferometric data, we recover 50%-70% flux loss due to phase errors. This allows us to restore more than 90% of a source flux. The method outlined in this work is not only applicable to the correction of deformation for other coplanar telescopes but also to single-dish telescopes with deformation problems. This work also forms the basis of the upcoming science results of AMiBA-13.

PLATFORM DEFORMATION PHASE CORRECTION FOR THE AMiBA-13 COPLANAR INTERFEROMETER

International Nuclear Information System (INIS)

Liao, Yu-Wei; Lin, Kai-Yang; Huang, Yau-De; Ho, Paul T. P.; Chen, Ming-Tang; Locutus Huang, Chih-Wei; Koch, Patrick M.; Nishioka, Hiroaki; Umetsu, Keiichi; Han, Chih-Chiang; Li, Chao-Te; Martin-Cocher, Pierre; Oshiro, Peter; Proty Wu, Jiun-Huei; Cheng, Tai-An; Fu, Szu-Yuan; Wang, Fu-Cheng; Liu, Guo-Chin; Molnar, Sandor M.; Chang, Yu-Yen

2013-01-01

We present a new way to solve the platform deformation problem of coplanar interferometers. The platform of a coplanar interferometer can be deformed due to driving forces and gravity. A deformed platform will induce extra components into the geometric delay of each baseline and change the phases of observed visibilities. The reconstructed images will also be diluted due to the errors of the phases. The platform deformations of The Yuan-Tseh Lee Array for Microwave Background Anisotropy (AMiBA) were modeled based on photogrammetry data with about 20 mount pointing positions. We then used the differential optical pointing error between two optical telescopes to fit the model parameters in the entire horizontal coordinate space. With the platform deformation model, we can predict the errors of the geometric phase delays due to platform deformation with a given azimuth and elevation of the targets and calibrators. After correcting the phases of the radio point sources in the AMiBA interferometric data, we recover 50%-70% flux loss due to phase errors. This allows us to restore more than 90% of a source flux. The method outlined in this work is not only applicable to the correction of deformation for other coplanar telescopes but also to single-dish telescopes with deformation problems. This work also forms the basis of the upcoming science results of AMiBA-13.

Platform Deformation Phase Correction for the AMiBA-13 Coplanar Interferometer

Science.gov (United States)

Liao, Yu-Wei; Lin, Kai-Yang; Huang, Yau-De; Proty Wu, Jiun-Huei; Ho, Paul T. P.; Chen, Ming-Tang; Locutus Huang, Chih-Wei; Koch, Patrick M.; Nishioka, Hiroaki; Cheng, Tai-An; Fu, Szu-Yuan; Liu, Guo-Chin; Molnar, Sandor M.; Umetsu, Keiichi; Wang, Fu-Cheng; Chang, Yu-Yen; Han, Chih-Chiang; Li, Chao-Te; Martin-Cocher, Pierre; Oshiro, Peter

2013-05-01

We present a new way to solve the platform deformation problem of coplanar interferometers. The platform of a coplanar interferometer can be deformed due to driving forces and gravity. A deformed platform will induce extra components into the geometric delay of each baseline and change the phases of observed visibilities. The reconstructed images will also be diluted due to the errors of the phases. The platform deformations of The Yuan-Tseh Lee Array for Microwave Background Anisotropy (AMiBA) were modeled based on photogrammetry data with about 20 mount pointing positions. We then used the differential optical pointing error between two optical telescopes to fit the model parameters in the entire horizontal coordinate space. With the platform deformation model, we can predict the errors of the geometric phase delays due to platform deformation with a given azimuth and elevation of the targets and calibrators. After correcting the phases of the radio point sources in the AMiBA interferometric data, we recover 50%-70% flux loss due to phase errors. This allows us to restore more than 90% of a source flux. The method outlined in this work is not only applicable to the correction of deformation for other coplanar telescopes but also to single-dish telescopes with deformation problems. This work also forms the basis of the upcoming science results of AMiBA-13.

Platform-based production development

DEFF Research Database (Denmark)

Bossen, Jacob; Brunoe, Thomas Ditlev; Nielsen, Kjeld

2015-01-01

Platforms as a means for applying modular thinking in product development is relatively well studied, but platforms in the production system has until now not been given much attention. With the emerging concept of platform-based co-development the importance of production platforms is though...

New Developments in Robotics and Single-site Gynecologic Surgery.

Science.gov (United States)

Matthews, Catherine A

2017-06-01

Within the last 10 years there have been significant advances in minimal-access surgery. Although no emerging technology has demonstrated improved outcomes or fewer complications than standard laparoscopy, the introduction of the robotic surgical platform has significantly lowered abdominal hysterectomy rates. While operative time and cost were higher in robotic-assisted procedures when the technology was first introduced, newer studies demonstrate equivalent or improved robotic surgical efficiency with increased experience. Single-port hysterectomy has not improved postoperative pain or subjective cosmetic results. Emerging platforms with flexible, articulating instruments may increase the uptake of single-port procedures including natural orifice transluminal endoscopic cases.

Space Situational Awareness of Large Numbers of Payloads From a Single Deployment

Science.gov (United States)

Segerman, A.; Byers, J.; Emmert, J.; Nicholas, A.

2014-09-01

The nearly simultaneous deployment of a large number of payloads from a single vehicle presents a new challenge for space object catalog maintenance and space situational awareness (SSA). Following two cubesat deployments last November, it took five weeks to catalog the resulting 64 orbits. The upcoming Kicksat mission will present an even greater SSA challenge, with its deployment of 128 chip-sized picosats. Although all of these deployments are in short-lived orbits, future deployments will inevitably occur at higher altitudes, with a longer term threat of collision with active spacecraft. With such deployments, individual scientific payload operators require rapid precise knowledge of their satellites' locations. Following the first November launch, the cataloguing did not initially associate a payload with each orbit, leaving this to the satellite operators. For short duration missions, the time required to identify an experiment's specific orbit may easily be a large fraction of the spacecraft's lifetime. For a Kicksat-type deployment, present tracking cannot collect enough observations to catalog each small object. The current approach is to treat the chip cloud as a single catalog object. However, the cloud dissipates into multiple subclouds and, ultimately, tiny groups of untrackable chips. One response to this challenge may be to mandate installation of a transponder on each spacecraft. Directional transponder transmission detections could be used as angle observations for orbit cataloguing. Of course, such an approach would only be employable with cooperative spacecraft. In other cases, a probabilistic association approach may be useful, with the goal being to establish the probability of an element being at a given point in space. This would permit more reliable assessment of the probability of collision of active spacecraft with any cloud element. This paper surveys the cataloguing challenges presented by large scale deployments of small spacecraft

Omnidirectional holonomic platforms

International Nuclear Information System (INIS)

Pin, F.G.; Killough, S.M.

1994-01-01

This paper presents the concepts for a new family of wheeled platforms which feature full omnidirectionality with simultaneous and independently controlled rotational and translational motion capabilities. The authors first present the orthogonal-wheels concept and the two major wheel assemblies on which these platforms are based. They then describe how a combination of these assemblies with appropriate control can be used to generate an omnidirectional capability for mobile robot platforms. The design and control of two prototype platforms are then presented and their respective characteristics with respect to rotational and translational motion control are discussed

Platform decommissioning costs

International Nuclear Information System (INIS)

Rodger, David

1998-01-01

There are over 6500 platforms worldwide contributing to the offshore oil and gas production industry. In the North Sea there are around 500 platforms in place. There are many factors to be considered in planning for platform decommissioning and the evaluation of options for removal and disposal. The environmental impact, technical feasibility, safety and cost factors all have to be considered. This presentation considers what information is available about the overall decommissioning costs for the North Sea and the costs of different removal and disposal options for individual platforms. 2 figs., 1 tab

Vaccine platform recombinant measles virus.

Science.gov (United States)

Mühlebach, Michael D

2017-10-01

The classic development of vaccines is lengthy, tedious, and may not necessarily be successful as demonstrated by the case of HIV. This is especially a problem for emerging pathogens that are newly introduced into the human population and carry the inherent risk of pandemic spread in a naïve population. For such situations, a considerable number of different platform technologies are under development. These are also under development for pathogens, where directly derived vaccines are regarded as too complicated or even dangerous due to the induction of inefficient or unwanted immune responses causing considerable side-effects as for dengue virus. Among platform technologies are plasmid-based DNA vaccines, RNA replicons, single-round infectious vector particles, or replicating vaccine-based vectors encoding (a) critical antigen(s) of the target pathogens. Among the latter, recombinant measles viruses derived from vaccine strains have been tested. Measles vaccines are among the most effective and safest life-attenuated vaccines known. Therefore, the development of Schwarz-, Moraten-, or AIK-C-strain derived recombinant vaccines against a wide range of mostly viral, but also bacterial pathogens was quite straightforward. These vaccines generally induce powerful humoral and cellular immune responses in appropriate animal models, i.e., transgenic mice or non-human primates. Also in the recent first clinical phase I trial, the results have been quite encouraging. The trial indicated the expected safety and efficacy also in human patients, interestingly independent from the level of prevalent anti-measles immunity before the trial. Thereby, recombinant measles vaccines expressing additional antigens are a promising platform for future vaccines.

Manufacture of an experimental platform with ECR ion source

International Nuclear Information System (INIS)

Zhou Changgeng; Hu Yonghong; Li Yan

2007-12-01

The working principle and basal configuration and fabricative process of ECR ion source are introdced. Regarding as an experimental and test device, the experimental platform of ECR ion source may expediently regulate every parameter of ion source, and achieve good character of beam current. Through improving on the components, ECR ion source can is modulated in best state. Above results may be used in the running and debugging of neutron generator. Therefore, the experimental platform of ECR ion source is the necessary equipment of large beam current neutron generator. Comparing the experimental platform of ECR ion source with domestic ones and the overseas ones, it mainly be used in the simulation experiments about neutron generator. It is compact and experimental platform mode in structure. It can focus the beam current and measure many parameters on line in function. The problem of lower beam current to discover is resolved in debugging of the device. The measurement results indicate that the technology character of the device have achieved design requirements. (authors)

Development of a thiol-ene based screening platform for enzyme immobilization demonstrated using horseradish peroxidase

DEFF Research Database (Denmark)

Hoffmann, Christian; Pinelo, Manuel; Woodley, John

2017-01-01

Efficient immobilization of enzymes on support surfaces requires an exact match between the surface chemistry and the specific enzyme. A successful match would normally be identified through time consuming screening of conventional resins in multiple experiments testing individual immobilization...... strategies. In this study we present a versatile strategy that largely expands the number of possible surface functionalities for enzyme immobilization in a single, generic platform. The combination of many individual surface chemistries and thus immobilization methods in one modular system permits faster...... functionalization by thiol-ene chemistry (TEC) resulted in the formation of a functional monolayer in each well, whereas, polymer surface grafts were introduced through surface chain transfer free radical polymerization (SCT-FRP). Enzyme immobilization on the modified surfaces was evaluated by using a rhodamine...

Retrograde Ureteroscopic Management of Large Renal Calculi: A Single Institutional Experience and Concise Literature Review.

Science.gov (United States)

Scotland, Kymora B; Rudnick, Benjamin; Healy, Kelly A; Hubosky, Scott G; Bagley, Demetrius H

2018-06-06

Advances in flexible ureteroscope design and accessory instrumentation have allowed for more challenging cases to be treated ureteroscopically. Here, we evaluate our experience with ureteroscopy (URS) for the management of large renal calculi (≥2 cm) and provide a concise review of recent reports. A retrospective review was undertaken of all URS cases between 2004 and 2014 performed by the endourologic team at a single academic tertiary care institution. We identified patients with at least one stone ≥2 cm managed with retrograde URS. Stone size was defined as the largest linear diameter of the index stone. Small diameter flexible ureteroscopes were used primarily with holmium laser. Patient demographics, intraoperative data, and postoperative outcomes were evaluated. We evaluated 167 consecutive patients who underwent URS for large renal stones ≥2 cm. The initial reason for choosing URS included patient preference (29.5%), failure of other therapies (8.2%), anatomic considerations/body habitus (30.3%), and comorbidities (28.8%). Mean patient age was 55.5 years (22-84). The mean stone size was 2.75 cm with mean number of procedures per patient of 1.65 (1-6). The single session stone-free rate was 57.1%, two-stage procedure stone-free rate was 90.2% and three-stage stone-free rate was 94.0%. Access sheaths were used in 47% of patients. An association was identified between stone size and patient outcomes; smaller stones correlated with decreased number of procedures. Postoperative complications were minor. Single or multi-stage retrograde ureteroscopic lithotripsy is a safe and effective mode of surgical management of large renal calculi. Total stone burden is a reliable predictor of the need for a staged procedure and of stone-free rate.

DNA Origami Directed Au Nanostar Dimers for Single-Molecule Surface-Enhanced Raman Scattering.

Science.gov (United States)

Tanwar, Swati; Haldar, Krishna Kanta; Sen, Tapasi

2017-12-06

We demonstrate the synthesis of Au nanostar dimers with tunable interparticle gap and controlled stoichiometry assembled on DNA origami. Au nanostars with uniform and sharp tips were immobilized on rectangular DNA origami dimerized structures to create nanoantennas containing monomeric and dimeric Au nanostars. Single Texas red (TR) dye was specifically attached in the junction of the dimerized origami to act as a Raman reporter molecule. The SERS enhancement factors of single TR dye molecules located in the conjunction region in dimer structures having interparticle gaps of 7 and 13 nm are 2 × 10 10 and 8 × 10 9 , respectively, which are strong enough for single analyte detection. The highly enhanced electromagnetic field generated by the plasmon coupling between sharp tips and cores of two Au nanostars in the wide conjunction region allows the accommodation and specific detection of large biomolecules. Such DNA-directed assembled nanoantennas with controlled interparticle separation distance and stoichiometry, and well-defined geometry, can be used as excellent substrates in single-molecule SERS spectroscopy and will have potential applications as a reproducible platform in single-molecule sensing.

Product Platform Replacements

DEFF Research Database (Denmark)

Sköld, Martin; Karlsson, Christer

2012-01-01

. To shed light on this unexplored and growing managerial concern, the purpose of this explorative study is to identify operational challenges to management when product platforms are replaced. Design/methodology/approach – The study uses a longitudinal field-study approach. Two companies, Gamma and Omega...... replacement was chosen in each company. Findings – The study shows that platform replacements primarily challenge managers' existing knowledge about platform architectures. A distinction can be made between “width” and “height” in platform replacements, and it is crucial that managers observe this in order...... to challenge their existing knowledge about platform architectures. Issues on technologies, architectures, components and processes as well as on segments, applications and functions are identified. Practical implications – Practical implications are summarized and discussed in relation to a framework...

3D shape measurement system developed on mobile platform

Science.gov (United States)

Wu, Zhoujie; Chang, Meng; Shi, Bowen; Zhang, Qican

2017-02-01

Three-dimensional (3-D) shape measurement technology based on structured light has become one hot research field inspired by the increasing requirements. Many methods have been implemented and applied in the industry applications, but most of their equipments are large and complex, cannot be portable. Meanwhile, the popularity of the smart mobile terminals, such as smart phones, provides a platform for the miniaturization and portability of this technology. The measurement system based on phase-shift algorithm and Gray-code pattern under the Android platform on a mobile phone is mainly studied and developed, and it has been encapsulated into a mobile phone application in order to reconstruct 3-D shape data in the employed smart phone easily and quickly. The experimental results of two measured object are given in this paper and demonstrate the application we developed in the mobile platform is effective.

Memory Hierarchy Design for Next Generation Scalable Many-core Platforms

OpenAIRE

Azarkhish, Erfan

2016-01-01

Performance and energy consumption in modern computing platforms is largely dominated by the memory hierarchy. The increasing computational power in the multiprocessors and accelerators, and the emergence of the data-intensive workloads (e.g. large-scale graph traversal and scientific algorithms) requiring fast transfer of large volumes of data, are two main trends which intensify this problem by putting even higher pressure on the memory hierarchy. This increasing gap between computation spe...

Development of Static Balance Measurement and Correction Compound Platform for Single Blade of Controllable Pitch Propeller

Science.gov (United States)

Chao, Zhang; Shijie, Su; Yilin, Yang; Guofu, Wang; Chao, Wang

2017-11-01

Aiming at the static balance of the controllable pitch propeller (CPP), a high efficiency static balance method based on the double-layer structure of the measuring table and gantry robot is adopted to realize the integration of torque measurement and corrected polish for controllable pitch propeller blade. The control system was developed by Microsoft Visual Studio 2015, and a composite platform prototype was developed. Through this prototype, conduct an experiment on the complete process of torque measurement and corrected polish based on a 300kg class controllable pitch propeller blade. The results show that the composite platform can correct the static balance of blade with a correct, efficient and labor-saving operation, and can replace the traditional method on static balance of the blade.

B-CAN: a resource sharing platform to improve the operation, visualization and integrated analysis of TCGA breast cancer data.

Science.gov (United States)

Wen, Can-Hong; Ou, Shao-Min; Guo, Xiao-Bo; Liu, Chen-Feng; Shen, Yan-Bo; You, Na; Cai, Wei-Hong; Shen, Wen-Jun; Wang, Xue-Qin; Tan, Hai-Zhu

2017-12-12

Breast cancer is a high-risk heterogeneous disease with myriad subtypes and complicated biological features. The Cancer Genome Atlas (TCGA) breast cancer database provides researchers with the large-scale genome and clinical data via web portals and FTP services. Researchers are able to gain new insights into their related fields, and evaluate experimental discoveries with TCGA. However, it is difficult for researchers who have little experience with database and bioinformatics to access and operate on because of TCGA's complex data format and diverse files. For ease of use, we build the breast cancer (B-CAN) platform, which enables data customization, data visualization, and private data center. The B-CAN platform runs on Apache server and interacts with the backstage of MySQL database by PHP. Users can customize data based on their needs by combining tables from original TCGA database and selecting variables from each table. The private data center is applicable for private data and two types of customized data. A key feature of the B-CAN is that it provides single table display and multiple table display. Customized data with one barcode corresponding to many records and processed customized data are allowed in Multiple Tables Display. The B-CAN is an intuitive and high-efficient data-sharing platform.

On Curating Multimodal Sensory Data for Health and Wellness Platforms

Directory of Open Access Journals (Sweden)

Muhammad Bilal Amin

2016-06-01

Full Text Available In recent years, the focus of healthcare and wellness technologies has shown a significant shift towards personal vital signs devices. The technology has evolved from smartphone-based wellness applications to fitness bands and smartwatches. The novelty of these devices is the accumulation of activity data as their users go about their daily life routine. However, these implementations are device specific and lack the ability to incorporate multimodal data sources. Data accumulated in their usage does not offer rich contextual information that is adequate for providing a holistic view of a user’s lifelog. As a result, making decisions and generating recommendations based on this data are single dimensional. In this paper, we present our Data Curation Framework (DCF which is device independent and accumulates a user’s sensory data from multimodal data sources in real time. DCF curates the context of this accumulated data over the user’s lifelog. DCF provides rule-based anomaly detection over this context-rich lifelog in real time. To provide computation and persistence over the large volume of sensory data, DCF utilizes the distributed and ubiquitous environment of the cloud platform. DCF has been evaluated for its performance, correctness, ability to detect complex anomalies, and management support for a large volume of sensory data.

On Curating Multimodal Sensory Data for Health and Wellness Platforms

Science.gov (United States)

Amin, Muhammad Bilal; Banos, Oresti; Khan, Wajahat Ali; Muhammad Bilal, Hafiz Syed; Gong, Jinhyuk; Bui, Dinh-Mao; Cho, Soung Ho; Hussain, Shujaat; Ali, Taqdir; Akhtar, Usman; Chung, Tae Choong; Lee, Sungyoung

2016-01-01

In recent years, the focus of healthcare and wellness technologies has shown a significant shift towards personal vital signs devices. The technology has evolved from smartphone-based wellness applications to fitness bands and smartwatches. The novelty of these devices is the accumulation of activity data as their users go about their daily life routine. However, these implementations are device specific and lack the ability to incorporate multimodal data sources. Data accumulated in their usage does not offer rich contextual information that is adequate for providing a holistic view of a user’s lifelog. As a result, making decisions and generating recommendations based on this data are single dimensional. In this paper, we present our Data Curation Framework (DCF) which is device independent and accumulates a user’s sensory data from multimodal data sources in real time. DCF curates the context of this accumulated data over the user’s lifelog. DCF provides rule-based anomaly detection over this context-rich lifelog in real time. To provide computation and persistence over the large volume of sensory data, DCF utilizes the distributed and ubiquitous environment of the cloud platform. DCF has been evaluated for its performance, correctness, ability to detect complex anomalies, and management support for a large volume of sensory data. PMID:27355955

The method of arbitrarily large moments to calculate single scale processes in quantum field theory

Energy Technology Data Exchange (ETDEWEB)

Bluemlein, Johannes [Deutsches Elektronen-Synchrotron (DESY), Zeuthen (Germany); Schneider, Carsten [Johannes Kepler Univ., Linz (Austria). Research Inst. for Symbolic Computation (RISC)

2017-01-15

We device a new method to calculate a large number of Mellin moments of single scale quantities using the systems of differential and/or difference equations obtained by integration-by-parts identities between the corresponding Feynman integrals of loop corrections to physical quantities. These scalar quantities have a much simpler mathematical structure than the complete quantity. A sufficiently large set of moments may even allow the analytic reconstruction of the whole quantity considered, holding in case of first order factorizing systems. In any case, one may derive highly precise numerical representations in general using this method, which is otherwise completely analytic.

Automated microfluidic platform for systematic studies of colloidal perovskite nanocrystals: towards continuous nano-manufacturing.

Science.gov (United States)

Epps, Robert W; Felton, Kobi C; Coley, Connor W; Abolhasani, Milad

2017-11-21

Colloidal organic/inorganic metal-halide perovskite nanocrystals have recently emerged as a potential low-cost replacement for the semiconductor materials in commercial photovoltaics and light emitting diodes. However, unlike III-V and IV-VI semiconductor nanocrystals, studies of colloidal perovskite nanocrystals have yet to develop a fundamental and comprehensive understanding of nucleation and growth kinetics. Here, we introduce a modular and automated microfluidic platform for the systematic studies of room-temperature synthesized cesium-lead halide perovskite nanocrystals. With abundant data collection across the entirety of four orders of magnitude reaction time span, we comprehensively characterize nanocrystal growth within a modular microfluidic reactor. The developed high-throughput screening platform features a custom-designed three-port flow cell with translational capability for in situ spectral characterization of the in-flow synthesized perovskite nanocrystals along a tubular microreactor with an adjustable length, ranging from 3 cm to 196 cm. The translational flow cell allows for sampling of twenty unique residence times at a single equilibrated flow rate. The developed technique requires an average total liquid consumption of 20 μL per spectra and as little as 2 μL at the time of sampling. It may continuously sample up to 30 000 unique spectra per day in both single and multi-phase flow formats. Using the developed plug-and-play microfluidic platform, we study the growth of cesium lead trihalide perovskite nanocrystals through in situ monitoring of their absorption and emission band-gaps at residence times ranging from 100 ms to 17 min. The automated microfluidic platform enables a systematic study of the effect of mixing enhancement on the quality of the synthesized nanocrystals through a direct comparison between single- and multi-phase flow systems at similar reaction time scales. The improved mixing characteristics of the multi-phase flow

Loads on Entrance Platforms for Offshore Wind Turbines

DEFF Research Database (Denmark)

Frigaard, Peter; Andersen, Thomas Lykke; Ramirez, Jorge Robert Rodriguez

2010-01-01

The present paper gives an overview of the performed large scale tests in GWK, Hannover for studying wave run-up generated forces on wind turbine entrance platforms. The run-up height and velocity was measured by use of high speed video recordings supplemented by some wave gauges mounted at the p...

Product Platform Modeling

DEFF Research Database (Denmark)

Pedersen, Rasmus

for customisation of products. In many companies these changes in the business environment have created a controversy between the need for a wide variety of products offered to the marketplace and a desire to reduce variation within the company in order to increase efficiency. Many companies use the concept...... other. These groups can be varied and combined to form different product variants without increasing the internal variety in the company. Based on the Theory of Domains, the concept of encapsulation in the organ domain is introduced, and organs are formulated as platform elements. Included......This PhD thesis has the title Product Platform Modelling. The thesis is about product platforms and visual product platform modelling. Product platforms have gained an increasing attention in industry and academia in the past decade. The reasons are many, yet the increasing globalisation...

Large-scale single-crystal growth of (CH3)2NH2CuCl3 for neutron scattering experiments

Science.gov (United States)

Park, Garam; Oh, In-Hwan; Park, J. M. Sungil; Park, Seong-Hun; Hong, Chang Seop; Lee, Kwang-Sei

2016-05-01

Neutron scattering studies on low-dimensional quantum spin systems require large-size single-crystals. Single-crystals of (CH3)2NH2CuCl3 showing low-dimensional magnetic behaviors were grown by a slow solvent evaporation method in a two-solvent system at different temperature settings. The best results were obtained for the bilayer solution of methanol and isopropanol with a molar ratio of 2:1 at 35 °C. The quality of the obtained single-crystals was tested by powder and single-crystal X-ray diffraction and single-crystal neutron diffraction. In addition, to confirm structural phase transitions (SPTs), thermal analysis and single-crystal X-ray diffraction at 300 K and 175 K, respectively, were conducted, confirming the presence of a SPT at Tup=288 K on heating and Tdown=285 K on cooling.

Microfluidic Lab-on-a-Chip Platforms: Requirements, Characteristics and Applications

Science.gov (United States)

Mark, D.; Haeberle, S.; Roth, G.; von Stetten, F.; Zengerle, R.

This review summarizes recent developments in microfluidic platform approaches. In contrast to isolated application-specific solutions, a microfluidic platform provides a set of fluidic unit operations, which are designed for easy combination within a well-defined fabrication technology. This allows the implementation of different application-specific (bio-) chemical processes, automated by microfluidic process integration [1]. A brief introduction into technical advances, major market segments and promising applications is followed by a detailed characterization of different microfluidic platforms, comprising a short definition, the functional principle, microfluidic unit operations, application examples as well as strengths and limitations. The microfluidic platforms in focus are lateral flow tests, linear actuated devices, pressure driven laminar flow, microfluidic large scale integration, segmented flow microfluidics, centrifugal microfluidics, electro-kinetics, electrowetting, surface acoustic waves, and systems for massively parallel analysis. The review concludes with the attempt to provide a selection scheme for microfluidic platforms which is based on their characteristics according to key requirements of different applications and market segments. Applied selection criteria comprise portability, costs of instrument and disposable, sample throughput, number of parameters per sample, reagent consumption, precision, diversity of microfluidic unit operations and the flexibility in programming different liquid handling protocols.

Automatic and integrated micro-enzyme assay (AIμEA) platform for highly sensitive thrombin analysis via an engineered fluorescence protein-functionalized monolithic capillary column.

Science.gov (United States)

Lin, Lihua; Liu, Shengquan; Nie, Zhou; Chen, Yingzhuang; Lei, Chunyang; Wang, Zhen; Yin, Chao; Hu, Huiping; Huang, Yan; Yao, Shouzhuo

2015-04-21

Nowadays, large-scale screening for enzyme discovery, engineering, and drug discovery processes require simple, fast, and sensitive enzyme activity assay platforms with high integration and potential for high-throughput detection. Herein, a novel automatic and integrated micro-enzyme assay (AIμEA) platform was proposed based on a unique microreaction system fabricated by a engineered green fluorescence protein (GFP)-functionalized monolithic capillary column, with thrombin as an example. The recombinant GFP probe was rationally engineered to possess a His-tag and a substrate sequence of thrombin, which enable it to be immobilized on the monolith via metal affinity binding, and to be released after thrombin digestion. Combined with capillary electrophoresis-laser-induced fluorescence (CE-LIF), all the procedures, including thrombin injection, online enzymatic digestion in the microreaction system, and label-free detection of the released GFP, were integrated in a single electrophoretic process. By taking advantage of the ultrahigh loading capacity of the AIμEA platform and the CE automatic programming setup, one microreaction column was sufficient for many times digestion without replacement. The novel microreaction system showed significantly enhanced catalytic efficiency, about 30 fold higher than that of the equivalent bulk reaction. Accordingly, the AIμEA platform was highly sensitive with a limit of detection down to 1 pM of thrombin. Moreover, the AIμEA platform was robust and reliable to detect thrombin in human serum samples and its inhibition by hirudin. Hence, this AIμEA platform exhibits great potential for high-throughput analysis in future biological application, disease diagnostics, and drug screening.

Bend compensated large-mode-area fibers: achieving robust single-modedness with transformation optics.

Science.gov (United States)

Fini, John M; Nicholson, Jeffrey W

2013-08-12

Fibers with symmetric bend compensated claddings are proposed, and demonstrate performance much better than conventional designs. These fibers can simultaneously achieve complete HOM suppression, negligible bend loss, and mode area >1000 square microns. The robust single-modedness of these fibers offers a path to overcoming mode instability limits on high-power amplifiers and lasers. The proposed designs achieve many of the advantages of our previous (asymmetric) bend compensation strategy in the regime of moderately large area, and are much easier to fabricate and utilize.

Introducing Platform Interactions Model for Studying Multi-Sided Platforms

DEFF Research Database (Denmark)

Staykova, Kalina; Damsgaard, Jan

2018-01-01

Multi-Sided Platforms (MSPs) function as socio-technical entities that facilitate direct interactions between various affiliated to them constituencies through developing and managing IT architecture. In this paper, we aim to explain the nature of the platform interactions as key characteristic o...

Immediate occlusal loading of single lower molars using Brånemark System® Wide Platform TiUnite™ implants: a 5-year follow-up report of a prospective clinical multicenter study.

Science.gov (United States)

Calandriello, Roberto; Tomatis, Massimiliano

2011-12-01

Although not essential, molars hold their importance in terms of functional jaw stability, antagonist opposition, and support of facial height. Therefore, implant therapy is an attractive concept in molar areas. However, especially in the posterior mandible, the conventional two-stage surgical approach to implant therapy was reported to cause higher bone loss and/or higher implant failures with machined implants because of the peculiar anatomic and physiologic conditions of this area. As the TiUnite™ (Nobel Biocare AB, Göteborg, Sweden) surface results in faster bone healing than with machined-surface implants, it was hypothesized that this surface would also improve the performance of wide implants in posterior mandibles. Based on these assumptions, a protocol for immediately loaded implants for single molar replacement was developed. This paper aimed to report on the clinical and radiological performance of Brånemark System® TiUnite Wide Platform implants supporting single molars in the lower jaw, loaded immediately and followed for up to 5 years, and to assess if the benefit delivered by oxidized surfaces in the short run is also present after 5 years. The study includes 33 consecutive patients treated between March 2001 and September 2003 and monitored until September 2008 in two private dental offices. A total of 40 Brånemark System TiUnite Wide Platform MK III implants were placed. All implants were provided with provisional crowns in full centric occlusion at the time of surgery. Patients were clinically and radiologically followed up for up to 5 years. Two implant failed so that the cumulative success rate at 5 years was 95.0%. The mean marginal bone remodeling (n = 38) expressed as mesial plus distal value averages was -1.17 mm (SD ± 0.90) at the 5-year time point. Although limited by the number of patients treated in accordance with the protocol described, 5-year results encourage the use of immediately loaded single lower molars supported by Br

Validation of the Gate simulation platform in single photon emission computed tomography and application to the development of a complete 3-dimensional reconstruction algorithm

International Nuclear Information System (INIS)

Lazaro, D.

2003-10-01

Monte Carlo simulations are currently considered in nuclear medical imaging as a powerful tool to design and optimize detection systems, and also to assess reconstruction algorithms and correction methods for degrading physical effects. Among the many simulators available, none of them is considered as a standard in nuclear medical imaging: this fact has motivated the development of a new generic Monte Carlo simulation platform (GATE), based on GEANT4 and dedicated to SPECT/PET (single photo emission computed tomography / positron emission tomography) applications. We participated during this thesis to the development of the GATE platform within an international collaboration. GATE was validated in SPECT by modeling two gamma cameras characterized by a different geometry, one dedicated to small animal imaging and the other used in a clinical context (Philips AXIS), and by comparing the results obtained with GATE simulations with experimental data. The simulation results reproduce accurately the measured performances of both gamma cameras. The GATE platform was then used to develop a new 3-dimensional reconstruction method: F3DMC (fully 3-dimension Monte-Carlo) which consists in computing with Monte Carlo simulation the transition matrix used in an iterative reconstruction algorithm (in this case, ML-EM), including within the transition matrix the main physical effects degrading the image formation process. The results obtained with the F3DMC method were compared to the results obtained with three other more conventional methods (FBP, MLEM, MLEMC) for different phantoms. The results of this study show that F3DMC allows to improve the reconstruction efficiency, the spatial resolution and the signal to noise ratio with a satisfactory quantification of the images. These results should be confirmed by performing clinical experiments and open the door to a unified reconstruction method, which could be applied in SPECT but also in PET. (author)

Low power radio communication platform for wireless sensor network

NARCIS (Netherlands)

Dutta, R.; Bentum, Marinus Jan; van der Zee, Ronan A.R.; Kokkeler, Andre B.J.

2009-01-01

Wireless sensor networks are predicted to be the most versatile, popular and useful technology in the near future. A large number of applications are targeted which will hugely benefit from a network of tiny computers with few sensors, radio communication platform, intelligent networking and

Unraveling the physics of nanofluidic phenomena at the single-molecule level

Energy Technology Data Exchange (ETDEWEB)

Fornasiero, Francesco [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2015-10-13

Despite groundbreaking potential in a broad application space, several nanofluidic phenomena remain poorly understood. Toward advancing the understanding of fluid behavior under nanoscale confinement, we developed a novel, ideal platform for fundamental molecular transport studies, in which the fluidic channel is a single carbon nanotube (CNT). CNTs offer the advantage of simple chemistry and structure, which can be synthetically tuned with nanometer precision and accurately modeled. With combined experimental and computational approaches, we demonstrated that CNT pores with 1-5 nm diameters conduct giant ionic currents that follow an unusual sublinear electrolyte concentration dependence. The large magnitude of the ionic conductance appears to originate from a strong electro-osmotic flow in smooth CNT pores. First-principle simulations suggest that electro-osmotic flow arises from localized negative polarization charges on carbon atoms near a potassium (K⁺) ion and from the strong cation-graphitic wall interactions, which drive K⁺ ions much closer to the wall than chlorides (Cl^-). Single-molecule translocation studies reveal that charged molecules may be distinguished from neutral species on the basis of the sign of the transient current change during their passage through the nanopore. Together with shedding light on a few controversial questions in the CNT nanofluidics area, these results may benefit LLNL’s Security Mission by providing the foundation for the development of advanced single-molecule detection system for bio/chem/explosive analytes. In addition, these experimental and computational platforms can be applied to advance fundamental knowledge in other fields, from energy storage and membrane separation to superfluid physics.

Focusing of white synchrotron radiation using large-acceptance cylindrical refractive lenses made of single – crystal diamond

Energy Technology Data Exchange (ETDEWEB)

Polikarpov, M., E-mail: polikarpov.maxim@mail.ru [Immanuel Kant Baltic Federal University, Nevskogo 14a, 23600 Kaliningrad (Russian Federation); Snigireva, I. [European Synchrotron Radiation Facility, 71 avenue des Martyrs, Grenoble 38043 (France); Snigirev, A. [Immanuel Kant Baltic Federal University, Nevskogo 14a, 23600 Kaliningrad (Russian Federation); European Synchrotron Radiation Facility, 71 avenue des Martyrs, Grenoble 38043 (France)

2016-07-27

Large-aperture cylindrical refractive lenses were manufactured by laser cutting of single-crystal diamond. Five linear single lenses with apertures of 1 mm and the depth of the structure of 1.2 mm were fabricated and tested at the ESRF ID06 beamline performing the focusing of white-beam synchrotron radiation. Uniform linear focus was stable during hours of exposure, representing such lenses as pre-focusing and collimating devices suitable for the front-end sections of today synchrotron radiation sources.

Focusing of white synchrotron radiation using large-acceptance cylindrical refractive lenses made of single – crystal diamond

International Nuclear Information System (INIS)

Polikarpov, M.; Snigireva, I.; Snigirev, A.

2016-01-01

Large-aperture cylindrical refractive lenses were manufactured by laser cutting of single-crystal diamond. Five linear single lenses with apertures of 1 mm and the depth of the structure of 1.2 mm were fabricated and tested at the ESRF ID06 beamline performing the focusing of white-beam synchrotron radiation. Uniform linear focus was stable during hours of exposure, representing such lenses as pre-focusing and collimating devices suitable for the front-end sections of today synchrotron radiation sources.

A Ubiquitous Sensor Network Platform for Integrating Smart Devices into the Semantic Sensor Web

Science.gov (United States)

de Vera, David Díaz Pardo; Izquierdo, Álvaro Sigüenza; Vercher, Jesús Bernat; Gómez, Luis Alfonso Hernández

2014-01-01

Ongoing Sensor Web developments make a growing amount of heterogeneous sensor data available to smart devices. This is generating an increasing demand for homogeneous mechanisms to access, publish and share real-world information. This paper discusses, first, an architectural solution based on Next Generation Networks: a pilot Telco Ubiquitous Sensor Network (USN) Platform that embeds several OGC® Sensor Web services. This platform has already been deployed in large scale projects. Second, the USN-Platform is extended to explore a first approach to Semantic Sensor Web principles and technologies, so that smart devices can access Sensor Web data, allowing them also to share richer (semantically interpreted) information. An experimental scenario is presented: a smart car that consumes and produces real-world information which is integrated into the Semantic Sensor Web through a Telco USN-Platform. Performance tests revealed that observation publishing times with our experimental system were well within limits compatible with the adequate operation of smart safety assistance systems in vehicles. On the other hand, response times for complex queries on large repositories may be inappropriate for rapid reaction needs. PMID:24945678

Single cell enzyme diagnosis on the chip

DEFF Research Database (Denmark)

Jensen, Sissel Juul; Harmsen, Charlotte; Nielsen, Mette Juul

2013-01-01

Conventional diagnosis based on ensemble measurements often overlooks the variation among cells. Here, we present a droplet-microfluidics based platform to investigate single cell activities. Adopting a previously developed isothermal rolling circle amplification-based assay, we demonstrate...... detection of enzymatic activities down to the single cell level with small quantities of biological samples, which outcompetes existing techniques. Such a system, capable of resolving single cell activities, will ultimately have clinical applications in diagnosis, prediction of drug response and treatment...

Ultra-high-density 3D DNA arrays within nanoporous biocompatible membranes for single-molecule-level detection and purification of circulating nucleic acids

Science.gov (United States)

Aramesh, M.; Shimoni, O.; Fox, K.; Karle, T. J.; Lohrmann, A.; Ostrikov, K.; Prawer, S.; Cervenka, J.

2015-03-01

Extracellular nucleic acids freely circulating in blood and other physiologic fluids are important biomarkers for non-invasive diagnostics and early detection of cancer and other diseases, yet difficult to detect because they exist in very low concentrations and large volumes. Here we demonstrate a new broad-range sensor platform for ultrasensitive and selective detection of circulating DNA down to the single-molecule level. The biosensor is based on a chemically functionalized nanoporous diamond-like carbon (DLC) coated alumina membrane. The few nanometer-thick, yet perfect and continuous DLC-coating confers the chemical stability and biocompatibility of the sensor, allowing its direct application in biological conditions. The selective detection is based on complementary hybridization of a fluorescently-tagged circulating cancer oncomarker (a 21-mer nucleic acid) with covalently immobilized DNA on the surface of the membrane. The captured DNAs are detected in the nanoporous structure of the sensor using confocal scanning laser microscopy. The flow-through membrane sensor demonstrates broad-range sensitivity, spanning from 1015 molecules per cm2 down to single molecules, which is several orders of magnitude improvement compared to the flat DNA microarrays. Our study suggests that these flow-through type nanoporous sensors represent a new powerful platform for large volume sampling and ultrasensitive detection of different chemical biomarkers.Extracellular nucleic acids freely circulating in blood and other physiologic fluids are important biomarkers for non-invasive diagnostics and early detection of cancer and other diseases, yet difficult to detect because they exist in very low concentrations and large volumes. Here we demonstrate a new broad-range sensor platform for ultrasensitive and selective detection of circulating DNA down to the single-molecule level. The biosensor is based on a chemically functionalized nanoporous diamond-like carbon (DLC) coated

A Cross-Platform Infrastructure for Scalable Runtime Application Performance Analysis

Energy Technology Data Exchange (ETDEWEB)

Jack Dongarra; Shirley Moore; Bart Miller, Jeffrey Hollingsworth; Tracy Rafferty

2005-03-15

The purpose of this project was to build an extensible cross-platform infrastructure to facilitate the development of accurate and portable performance analysis tools for current and future high performance computing (HPC) architectures. Major accomplishments include tools and techniques for multidimensional performance analysis, as well as improved support for dynamic performance monitoring of multithreaded and multiprocess applications. Previous performance tool development has been limited by the burden of having to re-write a platform-dependent low-level substrate for each architecture/operating system pair in order to obtain the necessary performance data from the system. Manual interpretation of performance data is not scalable for large-scale long-running applications. The infrastructure developed by this project provides a foundation for building portable and scalable performance analysis tools, with the end goal being to provide application developers with the information they need to analyze, understand, and tune the performance of terascale applications on HPC architectures. The backend portion of the infrastructure provides runtime instrumentation capability and access to hardware performance counters, with thread-safety for shared memory environments and a communication substrate to support instrumentation of multiprocess and distributed programs. Front end interfaces provides tool developers with a well-defined, platform-independent set of calls for requesting performance data. End-user tools have been developed that demonstrate runtime data collection, on-line and off-line analysis of performance data, and multidimensional performance analysis. The infrastructure is based on two underlying performance instrumentation technologies. These technologies are the PAPI cross-platform library interface to hardware performance counters and the cross-platform Dyninst library interface for runtime modification of executable images. The Paradyn and KOJAK

Single-particle motion in large-amplitude quadrupole shape transition

International Nuclear Information System (INIS)

Yamada, Kazuya

1991-01-01

The microscopic structure of the single-particle motion for the spherical-deformed transitional nuclei is analysed by using the self-consistent collective-coordinate method (SCC method). The single-particle motion in the moving-frame of reference called the collective vibrating coordinate frame is introduced by the generalized Bogoliubov transformation depending on the collective coordinate. The numerical calculations of the single-particle (quasi-particle) energy level diagrams and their occupation probabilities for the static deformation are carried out for the Sm isotopes. A clear change of the single-particle distribution structure appears in the course of deformation. (author)

Electrical Design and Evaluation of Asynchronous Serial Bus Communication Network of 48 Sensor Platform LSIs with Single-Ended I/O for Integrated MEMS-LSI Sensors

Science.gov (United States)

Shao, Chenzhong; Tanaka, Shuji; Nakayama, Takahiro; Hata, Yoshiyuki

2018-01-01

For installing many sensors in a limited space with a limited computing resource, the digitization of the sensor output at the site of sensation has advantages such as a small amount of wiring, low signal interference and high scalability. For this purpose, we have developed a dedicated Complementary Metal-Oxide-Semiconductor (CMOS) Large-Scale Integration (LSI) (referred to as “sensor platform LSI”) for bus-networked Micro-Electro-Mechanical-Systems (MEMS)-LSI integrated sensors. In this LSI, collision avoidance, adaptation and event-driven functions are simply implemented to relieve data collision and congestion in asynchronous serial bus communication. In this study, we developed a network system with 48 sensor platform LSIs based on Printed Circuit Board (PCB) in a backbone bus topology with the bus length being 2.4 m. We evaluated the serial communication performance when 48 LSIs operated simultaneously with the adaptation function. The number of data packets received from each LSI was almost identical, and the average sampling frequency of 384 capacitance channels (eight for each LSI) was 73.66 Hz. PMID:29342923

Electrical Design and Evaluation of Asynchronous Serial Bus Communication Network of 48 Sensor Platform LSIs with Single-Ended I/O for Integrated MEMS-LSI Sensors

Directory of Open Access Journals (Sweden)

Chenzhong Shao

2018-01-01

Full Text Available For installing many sensors in a limited space with a limited computing resource, the digitization of the sensor output at the site of sensation has advantages such as a small amount of wiring, low signal interference and high scalability. For this purpose, we have developed a dedicated Complementary Metal-Oxide-Semiconductor (CMOS Large-Scale Integration (LSI (referred to as “sensor platform LSI” for bus-networked Micro-Electro-Mechanical-Systems (MEMS-LSI integrated sensors. In this LSI, collision avoidance, adaptation and event-driven functions are simply implemented to relieve data collision and congestion in asynchronous serial bus communication. In this study, we developed a network system with 48 sensor platform LSIs based on Printed Circuit Board (PCB in a backbone bus topology with the bus length being 2.4 m. We evaluated the serial communication performance when 48 LSIs operated simultaneously with the adaptation function. The number of data packets received from each LSI was almost identical, and the average sampling frequency of 384 capacitance channels (eight for each LSI was 73.66 Hz.

Development of gamma-ray absorption and scattering simulation platform based on MCNP

International Nuclear Information System (INIS)

Lai Wanchang; Chen Henggui; Zhang Zhen; Chen Xiaoqiang

2010-01-01

It describes a γ-ray absorption and scattering simulation platform centering on MCNP, and developed corresponding accessories on the basis of the MCNP. Simulation of this simulation platform can be 93 kinds of single-quality materials and 2-3 kinds of multi-element mixture absorption experiment, simulating the absorption thickness of 0-100cm, and the thickness increment in 0.001cm. The media of Scattering Simulation is from the Li to the Am, the angle between the simulation measuring degree and incident ray direction is from-90 to 90, the angle in increments in 1 degree. (authors)

A Real-Time Simulation Platform for Power System Operation

DEFF Research Database (Denmark)

Cha, Seung-Tae; Østergaard, Jacob; Wu, Qiuwei

2010-01-01

This paper describes the real-time digital simulation platform that can be used for power system operation, analysis, and power system modeling. This particular platform gives grid operators, planners and researchers the opportunity to observe how a power system behaves and can be used...... in real time. Various phenomena commonly encountered when dealing with the two-area system is studied. Despite its small size, it mimics very closely the behavior of typical systems in actual operation. The electromagnetic transient type of simulation made in RSCAD enables the study of fast and detailed...... phenomena like single-phase faults in the two-area network and to observe their effects on a larger time scale. Also, the case study of 11 bus system with 5 generators has been also used and the results are presented....

Versatile Molecular Silver Ink Platform for Printed Flexible Electronics.

Science.gov (United States)

Kell, Arnold J; Paquet, Chantal; Mozenson, Olga; Djavani-Tabrizi, Iden; Deore, Bhavana; Liu, Xiangyang; Lopinski, Gregory P; James, Robert; Hettak, Khelifa; Shaker, Jafar; Momciu, Adrian; Ferrigno, Julie; Ferrand, Olivier; Hu, Jian Xiong; Lafrenière, Sylvie; Malenfant, Patrick R L

2017-05-24

A silver molecular ink platform formulated for screen, inkjet, and aerosol jet printing is presented. A simple formulation comprising silver neodecanoate, ethyl cellulose, and solvent provides improved performance versus that of established inks, yet with improved economics. Thin, screen-printed traces with exceptional electrical (molecular ink platform enables an aerosol jet-compatible ink that yields conductive features on glass with 2× bulk resistivity and strong adhesion to various plastic substrates. An inkjet formulation is also used to print top source/drain contacts and demonstrate printed high-mobility thin film transistors (TFTs) based on semiconducting single-walled carbon nanotubes. TFTs with mobility values of ∼25 cm 2 V -1 s -1 and current on/off ratios >10 4 were obtained, performance similar to that of evaporated metal contacts in analogous devices.

A study of wave forces on an offshore platform by direct CFD and Morison equation

Directory of Open Access Journals (Sweden)

Zhang D.

2015-01-01

The next step is the presentation of 3D multiphase RANS simulation of the wind-turbine platform in single-harmonic regular waves. Simulation results from full 3D simulation will be compared to the results from Morison’s equation. We are motivated by the challenges of a floating platform which has complex underwater geometry (e.g. tethered semi-submersible. In cases like this, our hypothesis is that Morison’s equation will result in inaccurate prediction of forces, due to the limitations of 2D coefficients of simple geometries, and that 3D multiphase RANS CFD will be required to generate reliable predictions of platform loads and motions.

Dysphagia is prevalent in patients with CPEO and single, large-scale deletions in mtDNA

DEFF Research Database (Denmark)

Pedersen, Gitte Hedermann; Løkken, Nicoline; Dahlqvist, Julia R.

2017-01-01

Background  The aim of this study was to assess the frequency of subjective and objective dysphagia in patients with chronic progressive external ophthalmoplegia (CPEO) due to single, large-scale deletions (LSDs) of mitochondrial DNA (mtDNA). Methods  Sixteen patients with CPEO and single LSDs...... and single LSDs of mtDNA had a prolonged cold-water test, including one with a PEG-tube, who was unable to perform the test, and nine patients reported subjective swallowing problems (56.3%). All mitochondrial myopathy patients in the control group had a normal duration of the cold-water test.  Conclusions......  The study shows that dysphagia is a common problem in patients with CPEO and LSDs of mtDNA. Dysphagia seems to be progressive with age as abnormal swallowing occurred preferentially in persons ≥ 45 years. The study shows that increased awareness of this symptom should be given to address appropriate...

The method of arbitrarily large moments to calculate single scale processes in quantum field theory

Directory of Open Access Journals (Sweden)

Johannes Blümlein

2017-08-01

Full Text Available We devise a new method to calculate a large number of Mellin moments of single scale quantities using the systems of differential and/or difference equations obtained by integration-by-parts identities between the corresponding Feynman integrals of loop corrections to physical quantities. These scalar quantities have a much simpler mathematical structure than the complete quantity. A sufficiently large set of moments may even allow the analytic reconstruction of the whole quantity considered, holding in case of first order factorizing systems. In any case, one may derive highly precise numerical representations in general using this method, which is otherwise completely analytic.

Descriptor Data Bank (DDB): A Cloud Platform for Multiperspective Modeling of Protein-Ligand Interactions.

Science.gov (United States)

Ashtawy, Hossam M; Mahapatra, Nihar R

2018-01-22

Protein-ligand (PL) interactions play a key role in many life processes such as molecular recognition, molecular binding, signal transmission, and cell metabolism. Examples of interaction forces include hydrogen bonding, hydrophobic effects, steric clashes, electrostatic contacts, and van der Waals attractions. Currently, a large number of hypotheses and perspectives to model these interaction forces are scattered throughout the literature and largely forgotten. Instead, had they been assembled and utilized collectively, they would have substantially improved the accuracy of predicting binding affinity of protein-ligand complexes. In this work, we present Descriptor Data Bank (DDB), a data-driven platform on the cloud for facilitating multiperspective modeling of PL interactions. DDB is an open-access hub for depositing, hosting, executing, and sharing descriptor extraction tools and data for a large number of interaction modeling hypotheses. The platform also implements a machine-learning (ML) toolbox for automatic descriptor filtering and analysis and scoring function (SF) fitting and prediction. The descriptor filtering module is used to filter out irrelevant and/or noisy descriptors and to produce a compact subset from all available features. We seed DDB with 16 diverse descriptor extraction tools developed in-house and collected from the literature. The tools altogether generate over 2700 descriptors that characterize (i) proteins, (ii) ligands, and (iii) protein-ligand complexes. The in-house descriptors we extract are protein-specific which are based on pairwise primary and tertiary alignment of protein structures followed by clustering and trilateration. We built and used DDB's ML library to fit SFs to the in-house descriptors and those collected from the literature. We then evaluated them on several data sets that were constructed to reflect real-world drug screening scenarios. We found that multiperspective SFs that were constructed using a large number

Clean Transfer of Large Graphene Single Crystals for High-Intactness Suspended Membranes and Liquid Cells.

Science.gov (United States)

Zhang, Jincan; Lin, Li; Sun, Luzhao; Huang, Yucheng; Koh, Ai Leen; Dang, Wenhui; Yin, Jianbo; Wang, Mingzhan; Tan, Congwei; Li, Tianran; Tan, Zhenjun; Liu, Zhongfan; Peng, Hailin

2017-07-01

The atomically thin 2D nature of suspended graphene membranes holds promising in numerous technological applications. In particular, the outstanding transparency to electron beam endows graphene membranes great potential as a candidate for specimen support of transmission electron microscopy (TEM). However, major hurdles remain to be addressed to acquire an ultraclean, high-intactness, and defect-free suspended graphene membrane. Here, a polymer-free clean transfer of sub-centimeter-sized graphene single crystals onto TEM grids to fabricate large-area and high-quality suspended graphene membranes has been achieved. Through the control of interfacial force during the transfer, the intactness of large-area graphene membranes can be as high as 95%, prominently larger than reported values in previous works. Graphene liquid cells are readily prepared by π-π stacking two clean single-crystal graphene TEM grids, in which atomic-scale resolution imaging and temporal evolution of colloid Au nanoparticles are recorded. This facile and scalable production of clean and high-quality suspended graphene membrane is promising toward their wide applications for electron and optical microscopy. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Testing methods of ECR ion source experimental platform

International Nuclear Information System (INIS)

Zhou Changgeng; Hu Yonghong; Li Yan

2006-12-01

The principle and structure of ECR ion source experimental platform were introduce. The testing methods of the parameters of single main component and the comprehensive parameters under the condition of certain beam current and beam spot diameter were summarized in process of manufacturing. Some appropriate testing dates were given. The existent questions (the parameters of plasma density in discharge chamber and accurate hydrogen flow, etc. can not be measured in operation) and resolutions were also put forward. (authors)

Surface growth mechanisms and structural faulting in the growth of large single and spherulitic titanosilicate ETS-4 crystals

Science.gov (United States)

Miraglia, Peter Q.; Yilmaz, Bilge; Warzywoda, Juliusz; Sacco, Albert

2004-10-01

Morphological, surface and crystallographic analyses of titanosilicate ETS-4 products, with diverse habits ranging from spherulitic particles composed of submicron crystallites to large single crystals, are presented. Pole figures revealed that crystal surfaces with a-, b- and c- axes corresponded to , and directions, respectively. Thus, technologically important 8-membered ring pores and titania chains in ETS-4 run along the b-axis of single crystals and terminate at the smallest crystal face. Height of the spiral growth steps observed on {1 0 0} and {0 0 1} surfaces corresponded to the interplanar spacings associated with their crystallographic orientation, and is equivalent to the thickness of building units that form the ETS-4 framework. Data suggest that the more viscous synthesis mixtures, with a large driving force for growth, increased the two- and three-dimensional nucleation, while limiting the transport of nutrients to the growth surface. These conditions increase the tendency for stacking fault formation on {1 0 0} surfaces and small angle branching, which eventually results in spherulitic growth. The growth of high quality ETS-4 single crystals (from less viscous synthesis mixtures) occurred at lower surface nucleation rates. Data suggest that these high quality, large crystals grew due to one-dimensional nucleation at spiral hillocks, and indicate that under these conditions un-faulted growth is preferred.

High-Throughput Tabular Data Processor - Platform independent graphical tool for processing large data sets.

Science.gov (United States)

Madanecki, Piotr; Bałut, Magdalena; Buckley, Patrick G; Ochocka, J Renata; Bartoszewski, Rafał; Crossman, David K; Messiaen, Ludwine M; Piotrowski, Arkadiusz

2018-01-01

High-throughput technologies generate considerable amount of data which often requires bioinformatic expertise to analyze. Here we present High-Throughput Tabular Data Processor (HTDP), a platform independent Java program. HTDP works on any character-delimited column data (e.g. BED, GFF, GTF, PSL, WIG, VCF) from multiple text files and supports merging, filtering and converting of data that is produced in the course of high-throughput experiments. HTDP can also utilize itemized sets of conditions from external files for complex or repetitive filtering/merging tasks. The program is intended to aid global, real-time processing of large data sets using a graphical user interface (GUI). Therefore, no prior expertise in programming, regular expression, or command line usage is required of the user. Additionally, no a priori assumptions are imposed on the internal file composition. We demonstrate the flexibility and potential of HTDP in real-life research tasks including microarray and massively parallel sequencing, i.e. identification of disease predisposing variants in the next generation sequencing data as well as comprehensive concurrent analysis of microarray and sequencing results. We also show the utility of HTDP in technical tasks including data merge, reduction and filtering with external criteria files. HTDP was developed to address functionality that is missing or rudimentary in other GUI software for processing character-delimited column data from high-throughput technologies. Flexibility, in terms of input file handling, provides long term potential functionality in high-throughput analysis pipelines, as the program is not limited by the currently existing applications and data formats. HTDP is available as the Open Source software (https://github.com/pmadanecki/htdp).

A versatile optical microscope for time-dependent single-molecule and single-particle spectroscopy

Science.gov (United States)

Li, Hao; Yang, Haw

2018-03-01

This work reports the design and implementation of a multi-function optical microscope for time-dependent spectroscopy on single molecules and single nanoparticles. It integrates the now-routine single-object measurements into one standalone platform so that no reconfiguration is needed when switching between different types of sample or spectroscopy modes. The illumination modes include evanescent field through total internal reflection, dark-field illumination, and epi-excitation onto a diffraction-limited spot suitable for confocal detection. The detection modes include spectrally resolved line imaging, wide-field imaging with dual-color capability, and two-color single-element photon-counting detection. The switch between different spectroscopy and data acquisition modes is fully automated and executed through computer programming. The capability of this microscope is demonstrated through selected proof-of-principle experiments.

A versatile optical microscope for time-dependent single-molecule and single-particle spectroscopy.

Science.gov (United States)

Li, Hao; Yang, Haw

2018-03-28

This work reports the design and implementation of a multi-function optical microscope for time-dependent spectroscopy on single molecules and single nanoparticles. It integrates the now-routine single-object measurements into one standalone platform so that no reconfiguration is needed when switching between different types of sample or spectroscopy modes. The illumination modes include evanescent field through total internal reflection, dark-field illumination, and epi-excitation onto a diffraction-limited spot suitable for confocal detection. The detection modes include spectrally resolved line imaging, wide-field imaging with dual-color capability, and two-color single-element photon-counting detection. The switch between different spectroscopy and data acquisition modes is fully automated and executed through computer programming. The capability of this microscope is demonstrated through selected proof-of-principle experiments.

Development of Guidelines for Use of Proton Single-Event Test Data to Bound Single-Event Effect Susceptibility Due to Light Ions

Data.gov (United States)

National Aeronautics and Space Administration — Conventional methods for Single-Event Effects (SEE) Hardness Assurance have proven difficult to adapt to Explorer, Cubesat and other risk tolerant platforms with...

Rainbow: a tool for large-scale whole-genome sequencing data analysis using cloud computing.

Science.gov (United States)

Zhao, Shanrong; Prenger, Kurt; Smith, Lance; Messina, Thomas; Fan, Hongtao; Jaeger, Edward; Stephens, Susan

2013-06-27

Technical improvements have decreased sequencing costs and, as a result, the size and number of genomic datasets have increased rapidly. Because of the lower cost, large amounts of sequence data are now being produced by small to midsize research groups. Crossbow is a software tool that can detect single nucleotide polymorphisms (SNPs) in whole-genome sequencing (WGS) data from a single subject; however, Crossbow has a number of limitations when applied to multiple subjects from large-scale WGS projects. The data storage and CPU resources that are required for large-scale whole genome sequencing data analyses are too large for many core facilities and individual laboratories to provide. To help meet these challenges, we have developed Rainbow, a cloud-based software package that can assist in the automation of large-scale WGS data analyses. Here, we evaluated the performance of Rainbow by analyzing 44 different whole-genome-sequenced subjects. Rainbow has the capacity to process genomic data from more than 500 subjects in two weeks using cloud computing provided by the Amazon Web Service. The time includes the import and export of the data using Amazon Import/Export service. The average cost of processing a single sample in the cloud was less than 120 US dollars. Compared with Crossbow, the main improvements incorporated into Rainbow include the ability: (1) to handle BAM as well as FASTQ input files; (2) to split large sequence files for better load balance downstream; (3) to log the running metrics in data processing and monitoring multiple Amazon Elastic Compute Cloud (EC2) instances; and (4) to merge SOAPsnp outputs for multiple individuals into a single file to facilitate downstream genome-wide association studies. Rainbow is a scalable, cost-effective, and open-source tool for large-scale WGS data analysis. For human WGS data sequenced by either the Illumina HiSeq 2000 or HiSeq 2500 platforms, Rainbow can be used straight out of the box. Rainbow is available

Use of the NetBeans Platform for NASA Robotic Conjunction Assessment Risk Analysis

Science.gov (United States)

Sabey, Nickolas J.

2014-01-01

The latest Java and JavaFX technologies are very attractive software platforms for customers involved in space mission operations such as those of NASA and the US Air Force. For NASA Robotic Conjunction Assessment Risk Analysis (CARA), the NetBeans platform provided an environment in which scalable software solutions could be developed quickly and efficiently. Both Java 8 and the NetBeans platform are in the process of simplifying CARA development in secure environments by providing a significant amount of capability in a single accredited package, where accreditation alone can account for 6-8 months for each library or software application. Capabilities either in use or being investigated by CARA include: 2D and 3D displays with JavaFX, parallelization with the new Streams API, and scalability through the NetBeans plugin architecture.

An Advanced Control Technique for Floating Offshore Wind Turbines Based on More Compact Barge Platforms

Directory of Open Access Journals (Sweden)

Joannes Olondriz

2018-05-01

Full Text Available Hydrodynamic Floating Offshore Wind Turbine (FOWT platform specifications are typically dominated by seaworthiness and maximum operating platform-pitch angle-related requirements. However, such specifications directly impact the challenge posed by an FOWT in terms of control design. The conventional FOWT systems are typically based on large, heavy floating platforms, which are less likely to suffer from the negative damping effect caused by the excessive coupling between blade-pitch control and platform-pitch motion. An advanced control technique is presented here to increase system stability for barge type platforms. Such a technique mitigates platform-pitch motions and improves the generator speed regulation, while maintaining blade-pitch activity and reducing blade and tower loads. The NREL’s 5MW + ITI Energy barge reference model is taken as a basis for this work. Furthermore, the capabilities of the proposed controller for performing with a more compact and less hydrodynamically stable barge platform is analysed, with encouraging results.

Hierarchical Parallel Matrix Multiplication on Large-Scale Distributed Memory Platforms

KAUST Repository

Quintin, Jean-Noel

2013-10-01

Matrix multiplication is a very important computation kernel both in its own right as a building block of many scientific applications and as a popular representative for other scientific applications. Cannon\\'s algorithm which dates back to 1969 was the first efficient algorithm for parallel matrix multiplication providing theoretically optimal communication cost. However this algorithm requires a square number of processors. In the mid-1990s, the SUMMA algorithm was introduced. SUMMA overcomes the shortcomings of Cannon\\'s algorithm as it can be used on a nonsquare number of processors as well. Since then the number of processors in HPC platforms has increased by two orders of magnitude making the contribution of communication in the overall execution time more significant. Therefore, the state of the art parallel matrix multiplication algorithms should be revisited to reduce the communication cost further. This paper introduces a new parallel matrix multiplication algorithm, Hierarchical SUMMA (HSUMMA), which is a redesign of SUMMA. Our algorithm reduces the communication cost of SUMMA by introducing a two-level virtual hierarchy into the two-dimensional arrangement of processors. Experiments on an IBM BlueGene/P demonstrate the reduction of communication cost up to 2.08 times on 2048 cores and up to 5.89 times on 16384 cores. © 2013 IEEE.

Hierarchical Parallel Matrix Multiplication on Large-Scale Distributed Memory Platforms

KAUST Repository

Quintin, Jean-Noel; Hasanov, Khalid; Lastovetsky, Alexey

2013-01-01

Matrix multiplication is a very important computation kernel both in its own right as a building block of many scientific applications and as a popular representative for other scientific applications. Cannon's algorithm which dates back to 1969 was the first efficient algorithm for parallel matrix multiplication providing theoretically optimal communication cost. However this algorithm requires a square number of processors. In the mid-1990s, the SUMMA algorithm was introduced. SUMMA overcomes the shortcomings of Cannon's algorithm as it can be used on a nonsquare number of processors as well. Since then the number of processors in HPC platforms has increased by two orders of magnitude making the contribution of communication in the overall execution time more significant. Therefore, the state of the art parallel matrix multiplication algorithms should be revisited to reduce the communication cost further. This paper introduces a new parallel matrix multiplication algorithm, Hierarchical SUMMA (HSUMMA), which is a redesign of SUMMA. Our algorithm reduces the communication cost of SUMMA by introducing a two-level virtual hierarchy into the two-dimensional arrangement of processors. Experiments on an IBM BlueGene/P demonstrate the reduction of communication cost up to 2.08 times on 2048 cores and up to 5.89 times on 16384 cores. © 2013 IEEE.

Hybrid platform. Economical hybrid drive for commercial vehicles; Hybrid Plattform. Wirtschaftlicher Hybridantrieb fuer Nutzfahrzeuge

Energy Technology Data Exchange (ETDEWEB)

Wallner, S.; Lamke, M.; Mohr, M.; Sedlacek, M.; Speck, F.D. [ZF Friedrichshafen AG, Friedrichshafen (Germany)

2011-07-01

Up to now, hybrid systems have been adapted to their specific requirements in the various applications for trucks, buses as well as mobile and building machines. From a technical point of view, this does indeed result in optimized hybrid drives for each single vehicle application, but due to small volumes, such single developments are critical from a business point of view. ZF Friedrichshafen AG is providing a solution to the technical and economical requirements of the cost-sensitive CV segment in the form of a modular CV parallel hybrid platform composed of a hybrid module system, an inverter, a battery system, and a hybrid software integrated into the overall vehicle. Thanks to the intelligent combination of assemblies and the use of as many identical parts as possible, platforms are realized which cover power ranges between 60 and 120 kW, voltage ranges between 350 and 650 V, and battery capacities between 2 and 4 kWh. The dimensions of the platform elements are such that integration into the diverse commercial vehicle applications is made easy. The hybrid software required for the vehicle-specific functions is also configurable for the mentioned CV applications. (orig.)

Homemade Buckeye-Pi: A Learning Many-Node Platform for High-Performance Parallel Computing

Science.gov (United States)

Amooie, M. A.; Moortgat, J.

2017-12-01

We report on the "Buckeye-Pi" cluster, the supercomputer developed in The Ohio State University School of Earth Sciences from 128 inexpensive Raspberry Pi (RPi) 3 Model B single-board computers. Each RPi is equipped with fast Quad Core 1.2GHz ARMv8 64bit processor, 1GB of RAM, and 32GB microSD card for local storage. Therefore, the cluster has a total RAM of 128GB that is distributed on the individual nodes and a flash capacity of 4TB with 512 processors, while it benefits from low power consumption, easy portability, and low total cost. The cluster uses the Message Passing Interface protocol to manage the communications between each node. These features render our platform the most powerful RPi supercomputer to date and suitable for educational applications in high-performance-computing (HPC) and handling of large datasets. In particular, we use the Buckeye-Pi to implement optimized parallel codes in our in-house simulator for subsurface media flows with the goal of achieving a massively-parallelized scalable code. We present benchmarking results for the computational performance across various number of RPi nodes. We believe our project could inspire scientists and students to consider the proposed unconventional cluster architecture as a mainstream and a feasible learning platform for challenging engineering and scientific problems.

WiseEye: Next Generation Expandable and Programmable Camera Trap Platform for Wildlife Research.

Directory of Open Access Journals (Sweden)

Sajid Nazir

Full Text Available The widespread availability of relatively cheap, reliable and easy to use digital camera traps has led to their extensive use for wildlife research, monitoring and public outreach. Users of these units are, however, often frustrated by the limited options for controlling camera functions, the generation of large numbers of images, and the lack of flexibility to suit different research environments and questions. We describe the development of a user-customisable open source camera trap platform named 'WiseEye', designed to provide flexible camera trap technology for wildlife researchers. The novel platform is based on a Raspberry Pi single-board computer and compatible peripherals that allow the user to control its functions and performance. We introduce the concept of confirmatory sensing, in which the Passive Infrared triggering is confirmed through other modalities (i.e. radar, pixel change to reduce the occurrence of false positives images. This concept, together with user-definable metadata, aided identification of spurious images and greatly reduced post-collection processing time. When tested against a commercial camera trap, WiseEye was found to reduce the incidence of false positive images and false negatives across a range of test conditions. WiseEye represents a step-change in camera trap functionality, greatly increasing the value of this technology for wildlife research and conservation management.

Brief: Refloating Maureen platform for reuse in waters away from the North Sea

International Nuclear Information System (INIS)

Tilling, G.M.

1996-01-01

The Maureen platform in the U.K. sector of the North Sea is refloatable by virtue of being a steel, gravity-base structure with large oil storage tanks as legs. The fields producing into the Maureen platform will soon be depleted, and plans are to decommission the platform from its present location. The attractive disposal options all involve refloating the platform as a first step. Of these, reuse of the facility is potentially the most attractive. Once afloat, the platform becomes a towable 110,000-tonne vessel available for use in areas of the world away from the North Sea, with location determined by environmental conditions and water depth. The bulk of the weight (92,000 tonnes) is in the jacket structure; topsides weight is 18,000 tonnes. Subject to detailed tow-route and naval-stability calculations, moving the structure long distances appears to be feasible. This paper discusses the refloating and marine stability of the Maureen oil platform as a vessel under tow. Development and maintenance aspects are presented

Low-cost Citizen Science Balloon Platform for Measuring Air Pollutants to Improve Satellite Retrieval Algorithms

Science.gov (United States)

Potosnak, M. J.; Beck-Winchatz, B.; Ritter, P.

2016-12-01

High-altitude balloons (HABs) are an engaging platform for citizen science and formal and informal STEM education. However, the logistics of launching, chasing and recovering a payload on a 1200 g or 1500 g balloon can be daunting for many novice school groups and citizen scientists, and the cost can be prohibitive. In addition, there are many interesting scientific applications that do not require reaching the stratosphere, including measuring atmospheric pollutants in the planetary boundary layer. With a large number of citizen scientist flights, these data can be used to constrain satellite retrieval algorithms. In this poster presentation, we discuss a novel approach based on small (30 g) balloons that are cheap and easy to handle, and low-cost tracking devices (SPOT trackers for hikers) that do not require a radio license. Our scientific goal is to measure air quality in the lower troposphere. For example, particulate matter (PM) is an air pollutant that varies on small spatial scales and has sources in rural areas like biomass burning and farming practices such as tilling. Our HAB platform test flight incorporates an optical PM sensor, an integrated single board computer that records the PM sensor signal in addition to flight parameters (pressure, location and altitude), and a low-cost tracking system. Our goal is for the entire platform to cost less than $500. While the datasets generated by these flights are typically small, integrating a network of flight data from citizen scientists into a form usable for comparison to satellite data will require big data techniques.

Single nucleotide polymorphism (SNP) detection on a magnetoresistive sensor

DEFF Research Database (Denmark)

Rizzi, Giovanni; Østerberg, Frederik Westergaard; Dufva, Martin

2013-01-01

We present a magnetoresistive sensor platform for hybridization assays and demonstrate its applicability on single nucleotide polymorphism (SNP) genotyping. The sensor relies on anisotropic magnetoresistance in a new geometry with a local negative reference and uses the magnetic field from...... the sensor bias current to magnetize magnetic beads in the vicinity of the sensor. The method allows for real-time measurements of the specific bead binding to the sensor surface during DNA hybridization and washing. Compared to other magnetic biosensing platforms, our approach eliminates the need...... for external electromagnets and thus allows for miniaturization of the sensor platform....

A neurorobotic platform for locomotor prosthetic development in rats and mice

Science.gov (United States)

von Zitzewitz, Joachim; Asboth, Leonie; Fumeaux, Nicolas; Hasse, Alexander; Baud, Laetitia; Vallery, Heike; Courtine, Grégoire

2016-04-01

Objectives. We aimed to develop a robotic interface capable of providing finely-tuned, multidirectional trunk assistance adjusted in real-time during unconstrained locomotion in rats and mice. Approach. We interfaced a large-scale robotic structure actuated in four degrees of freedom to exchangeable attachment modules exhibiting selective compliance along distinct directions. This combination allowed high-precision force and torque control in multiple directions over a large workspace. We next designed a neurorobotic platform wherein real-time kinematics and physiological signals directly adjust robotic actuation and prosthetic actions. We tested the performance of this platform in both rats and mice with spinal cord injury. Main Results. Kinematic analyses showed that the robotic interface did not impede locomotor movements of lightweight mice that walked freely along paths with changing directions and height profiles. Personalized trunk assistance instantly enabled coordinated locomotion in mice and rats with severe hindlimb motor deficits. Closed-loop control of robotic actuation based on ongoing movement features enabled real-time control of electromyographic activity in anti-gravity muscles during locomotion. Significance. This neurorobotic platform will support the study of the mechanisms underlying the therapeutic effects of locomotor prosthetics and rehabilitation using high-resolution genetic tools in rodent models.

Single Photon Avalanche Diodes: Towards the Large Bidimensional Arrays

Directory of Open Access Journals (Sweden)

Emilio Sciacca

2008-08-01

Full Text Available Single photon detection is one of the most challenging goals of photonics. In recent years, the study of ultra-fast and/or low-intensity phenomena has received renewed attention from the academic and industrial communities. Intense research activity has been focused on bio-imaging applications, bio-luminescence, bio-scattering methods, and, more in general, on several applications requiring high speed operation and high timing resolution. In this paper we present design and characterization of bi-dimensional arrays of a next generation of single photon avalanche diodes (SPADs. Single photon sensitivity, dark noise, afterpulsing and timing resolution of the single SPAD have been examined in several experimental conditions. Moreover, the effects arising from their integration and the readout mode have also been deeply investigated.

Data Platforms and Cities

DEFF Research Database (Denmark)

Blok, Anders; Courmont, Antoine; Hoyng, Rolien

2017-01-01

This section offers a series of joint reflections on (open) data platform from a variety of cases, from cycling, traffic and mapping to activism, environment and data brokering. Data platforms play a key role in contemporary urban governance. Linked to open data initiatives, such platforms are of...

Rapid Growth of Large Single-Crystalline Graphene via Second Passivation and Multistage Carbon Supply.

Science.gov (United States)

Lin, Li; Sun, Luzhao; Zhang, Jincan; Sun, Jingyu; Koh, Ai Leen; Peng, Hailin; Liu, Zhongfan

2016-06-01

A second passivation and a multistage carbon-source supply (CSS) allow a 50-fold enhancement of the growth rate of large single-crystalline graphene with a record growth rate of 101 μm min(-1) , almost 10 times higher than for pure copper. To this end the CSS is tailored at separate stages of graphene growth on copper foil, combined with an effective suppression of new spontaneous nucleation via second passivation. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Mobile Platforms and Development Environments

CERN Document Server

Helal, Sumi; Li, Wengdong

2012-01-01

Mobile platform development has lately become a technological war zone with extremely dynamic and fluid movement, especially in the smart phone and tablet market space. This Synthesis lecture is a guide to the latest developments of the key mobile platforms that are shaping the mobile platform industry. The book covers the three currently dominant native platforms -- iOS, Android and Windows Phone -- along with the device-agnostic HTML5 mobile web platform. The lecture also covers location-based services (LBS) which can be considered as a platform in its own right. The lecture utilizes a sampl

Designing next-generation platforms for evaluating scientific output: What scientists can learn from the social web

Directory of Open Access Journals (Sweden)

Tal eYarkoni

2012-10-01

Full Text Available Traditional pre-publication peer review of scientific output is a slow, inefficient, and unreliable process. Efforts to replace or supplement traditional evaluation models with open evaluation platforms that leverage advances in information technology are slowly gaining traction, but remain in the early stages of design and implementation. Here I discuss a number of considerations relevant to the development of such platforms. I focus particular attention on three core elements that next-generation evaluation platforms should strive to emphasize, including (a open and transparent access to accumulated evaluation data, (b personalized and highly customizable performance metrics, and (c appropriate short-term incentivization of the userbase. Because all of these elements have already been successfully implemented on a large scale in hundreds of existing social web applications, I argue that development of new scientific evaluation platforms should proceed largely by adapting existing techniques rather than engineering entirely new evaluation mechanisms. Successful implementation of open evaluation platforms has the potential to substantially advance both the pace and the quality of scientific publication and evaluation, and the scientific community has a vested interest in shifting towards such models as soon as possible.

ITS Platform North Denmark

DEFF Research Database (Denmark)

Lahrmann, Harry; Agerholm, Niels; Juhl, Jens

2012-01-01

This paper presents the project entitled “ITS Platform North Denmark” which is used as a test platform for Intelligent Transportation System (ITS) solutions. The platform consists of a newly developed GNSS/GPRS On Board Unit (OBU) to be installed in 500 cars, a backend server and a specially...

Platform development supportedby gaming

DEFF Research Database (Denmark)

Mikkola, Juliana Hsuan; Hansen, Poul H. Kyvsgård

2007-01-01

The challenge of implementing industrial platforms in practice can be described as a configuration problem caused by high number of variables, which often have contradictory influences on the total performance of the firm. Consequently, the specific platform decisions become extremely complex......, possibly increasing the strategic risks for the firm. This paper reports preliminary findings on platform management process at LEGO, a Danish toy company.  Specifically, we report the process of applying games combined with simulations and workshops in the platform development. We also propose a framework...

Waveguide-Coupled Superconducting Nanowire Single-Photon Detectors

Science.gov (United States)

Beyer, Andrew D.; Briggs, Ryan M.; Marsili, Francesco; Cohen, Justin D.; Meenehan, Sean M.; Painter, Oskar J.; Shaw, Matthew D.

2015-01-01

We have demonstrated WSi-based superconducting nanowire single-photon detectors coupled to SiNx waveguides with integrated ring resonators. This photonics platform enables the implementation of robust and efficient photon-counting detectors with fine spectral resolution near 1550 nm.

Universal SaaS platform of internet of things for real-time monitoring

Science.gov (United States)

Liu, Tongke; Wu, Gang

2018-04-01

Real-time monitoring service, as a member of the IoT (Internet of Things) service, has a wide range application scenario. To support rapid construction and deployment of applications and avoid repetitive development works in these processes, this paper designs and develops a universal SaaS platform of IoT for real-time monitoring. Evaluation shows that this platform can provide SaaS service to multiple tenants and achieve high real-time performance under the situation of large amount of device access.

The Rise of Ridesharing Platforms: an Uber-assessment of Bits and atoms

OpenAIRE

Ofstad, Magnus

2017-01-01

This thesis is a case study of Uber. The research questions are 1. How do ridesharing platforms evolve from startups into large-scale market disrupters? 2. How can ridesharing platforms augment its user base and capture more segments within the transportation business? Uber’s scaling is traditional. The initial growth consisted of capturing the high end of the market. Later on, Uber moved down market, making ridesharing affordable for the masses. The master stroke was to devise a scalable bus...

Large-Scale Mixed Temperate Forest Mapping at the Single Tree Level using Airborne Laser Scanning

Science.gov (United States)

Scholl, V.; Morsdorf, F.; Ginzler, C.; Schaepman, M. E.

2017-12-01

Monitoring vegetation on a single tree level is critical to understand and model a variety of processes, functions, and changes in forest systems. Remote sensing technologies are increasingly utilized to complement and upscale the field-based measurements of forest inventories. Airborne laser scanning (ALS) systems provide valuable information in the vertical dimension for effective vegetation structure mapping. Although many algorithms exist to extract single tree segments from forest scans, they are often tuned to perform well in homogeneous coniferous or deciduous areas and are not successful in mixed forests. Other methods are too computationally expensive to apply operationally. The aim of this study was to develop a single tree detection workflow using leaf-off ALS data for the canton of Aargau in Switzerland. Aargau covers an area of over 1,400km2 and features mixed forests with various development stages and topography. Forest type was classified using random forests to guide local parameter selection. Canopy height model-based treetop maxima were detected and maintained based on the relationship between tree height and window size, used as a proxy to crown diameter. Watershed segmentation was used to generate crown polygons surrounding each maximum. The location, height, and crown dimensions of single trees were derived from the ALS returns within each polygon. Validation was performed through comparison with field measurements and extrapolated estimates from long-term monitoring plots of the Swiss National Forest Inventory within the framework of the Swiss Federal Institute for Forest, Snow, and Landscape Research. This method shows promise for robust, large-scale single tree detection in mixed forests. The single tree data will aid ecological studies as well as forest management practices. Figure description: Height-normalized ALS point cloud data (top) and resulting single tree segments (bottom) on the Laegeren mountain in Switzerland.

Near Real-time Scientific Data Analysis and Visualization with the ArcGIS Platform

Science.gov (United States)

Shrestha, S. R.; Viswambharan, V.; Doshi, A.

2017-12-01

Scientific multidimensional data are generated from a variety of sources and platforms. These datasets are mostly produced by earth observation and/or modeling systems. Agencies like NASA, NOAA, USGS, and ESA produce large volumes of near real-time observation, forecast, and historical data that drives fundamental research and its applications in larger aspects of humanity from basic decision making to disaster response. A common big data challenge for organizations working with multidimensional scientific data and imagery collections is the time and resources required to manage and process such large volumes and varieties of data. The challenge of adopting data driven real-time visualization and analysis, as well as the need to share these large datasets, workflows, and information products to wider and more diverse communities, brings an opportunity to use the ArcGIS platform to handle such demand. In recent years, a significant effort has put in expanding the capabilities of ArcGIS to support multidimensional scientific data across the platform. New capabilities in ArcGIS to support scientific data management, processing, and analysis as well as creating information products from large volumes of data using the image server technology are becoming widely used in earth science and across other domains. We will discuss and share the challenges associated with big data by the geospatial science community and how we have addressed these challenges in the ArcGIS platform. We will share few use cases, such as NOAA High Resolution Refresh Radar (HRRR) data, that demonstrate how we access large collections of near real-time data (that are stored on-premise or on the cloud), disseminate them dynamically, process and analyze them on-the-fly, and serve them to a variety of geospatial applications. We will also share how on-the-fly processing using raster functions capabilities, can be extended to create persisted data and information products using raster analytics

Large-scale synthesis of single-crystalline MgO with bone-like nanostructures

International Nuclear Information System (INIS)

Niu Haixia; Yang Qing; Tang Kaibin; Xie Yi

2006-01-01

Uniform bone-like MgO nanocrystals have been prepared via a solvothermal process using commercial Mg powders as the starting material in the absence of any catalyst or surfactant followed by a subsequent calcination. Field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) measurements indicate that the product consists of a large quantity of bone-like nanocrystals with lengths of 120-200 nm. The widths of these nanocrystals at both ends are in the range of 20-50 nm, which are 3-20 nm wider than those of the middle parts. Explorations of X-ray diffraction (XRD) and selected area electronic diffraction (SAED) exhibit that the product is high-quality cubic single-crystalline nanocrystals. The photoluminescence (PL) measurement suggests that the product has an intensive emission centered at 410 nm, showing that the product has potential application in optical devices. The advantages of our method lie in high yield, the easy availability of the starting materials and permitting large-scale production at low cost. The growth mechanism was proposed to be related with solvent's oxidation in the precursor formation process and following nucleation and mass-transfer in the decomposition of the precursor

An Ultra-Efficient Nonlinear Platform: AlGaAs-On-Insulator

DEFF Research Database (Denmark)

Pu, Minhao; Ottaviano, Luisa; Semenova, Elizaveta

The combination of nonlinear and integrated photonics enables applications including optical signal processing, multi-wavelength lasers, metrology, spectroscopy, and quantum information science. Silicon-on-insulator (SOI) has emerged as a promising platform [1, 2] due to its high material...... nonlinearity and its compatibility with the CMOS industry. However, silicon suffers two-photon absorption (TPA) in the telecommunication wavelength band around 1.55 µm, which hampers its applications. Different platforms have been proposed to avoid TPA in the telecom wavelength range such as Si3N4 and Hydex [3...... a nonlinear index (n2) on the order of 10−17 W/m2 and a high refractive index (n ≈3.3), a large transparency window (from near- to mid-infrared), and the ability to engineer the material bandgap to mitigate TPA [5]. In this presentation, we introduce AlGaAson-insulator (AlGaAsOI) platform which combines both...

Recurrence in Benign Paroxysmal Positional Vertigo: A Large, Single-Institution Study.

Science.gov (United States)

Luryi, Alexander L; Lawrence, Juliana; Bojrab, Dennis I; LaRouere, Michael; Babu, Seilesh; Zappia, John; Sargent, Eric W; Chan, Eleanor; Naumann, Ilka; Hong, Robert S; Schutt, Christopher A

2018-04-11

To report rates of recurrence in benign paroxysmal positional vertigo (BPPV) and associated patient and disease factors. Retrospective chart review. Single high-volume otology practice. Patients diagnosed with BPPV from 2007 to 2016 with documented resolution of symptoms. Diagnostic and particle repositioning maneuvers for BPPV. BPPV recurrence, time to recurrence, and ear(s) affected at recurrence. A total of 1,105 patients meeting criteria were identified. Of this population, 37% had recurrence of BPPV in either ear or both ears. Overall same-ear recurrence rate was 28%; 76% of recurrences involved the same ear(s) as initial presentation. Recurrences that occurred after longer disease-free intervals were more likely to involve the opposite ear than early recurrences (p = 0.02). Female sex (40.4% versus 32.7%, p = 0.01) and history of previous BPPV (57.5% versus 32.4%, p diabetes mellitus, and traumatic etiology were not. Approximately, half (56%) of recurrences occurred within 1 year of resolution. A large single-institution study of recurrence in BPPV is presented along with Kaplan-Meier disease-free survival curves. Female sex and history of previous BPPV were associated with increased recurrence, while previously suspected risk factors for recurrence including history of Menière's disease, diabetes, and trauma were not. Remote recurrence is more likely to involve the contralateral ear than early recurrence. These data solidify the expected course of treated BPPV allowing for improved clinical care and patient counseling.

A Multi-Technology Communication Platform for Urban Mobile Sensing.

Science.gov (United States)

Almeida, Rodrigo; Oliveira, Rui; Luís, Miguel; Senna, Carlos; Sargento, Susana

2018-04-12

A common concern in smart cities is the focus on sensing procedures to provide city-wide information to city managers and citizens. To meet the growing demands of smart cities, the network must provide the ability to handle a large number of mobile sensors/devices, with high heterogeneity and unpredictable mobility, by collecting and delivering the sensed information for future treatment. This work proposes a multi-wireless technology communication platform for opportunistic data gathering and data exchange with respect to smart cities. Through the implementation of a proprietary long-range (LoRa) network and an urban sensor network, our platform addresses the heterogeneity of Internet of Things (IoT) devices while conferring communications in an opportunistic manner, increasing the interoperability of our platform. It implements and evaluates a medium access communication (MAC) protocol for LoRa networks with multiple gateways. It also implements mobile Opportunistic VEhicular (mOVE), a delay-tolerant network (DTN)-based architecture to address the mobility dimension. The platform provides vehicle-to-everything (V2X) communication with support for highly reliable and actionable information flows. Moreover, taking into account the high mobility pattern that a smart city scenario presents, we propose and evaluate two forwarding strategies for the opportunistic sensor network.

A Multi-Technology Communication Platform for Urban Mobile Sensing

Directory of Open Access Journals (Sweden)

Rodrigo Almeida

2018-04-01

Full Text Available A common concern in smart cities is the focus on sensing procedures to provide city-wide information to city managers and citizens. To meet the growing demands of smart cities, the network must provide the ability to handle a large number of mobile sensors/devices, with high heterogeneity and unpredictable mobility, by collecting and delivering the sensed information for future treatment. This work proposes a multi-wireless technology communication platform for opportunistic data gathering and data exchange with respect to smart cities. Through the implementation of a proprietary long-range (LoRa network and an urban sensor network, our platform addresses the heterogeneity of Internet of Things (IoT devices while conferring communications in an opportunistic manner, increasing the interoperability of our platform. It implements and evaluates a medium access communication (MAC protocol for LoRa networks with multiple gateways. It also implements mobile Opportunistic VEhicular (mOVE, a delay-tolerant network (DTN-based architecture to address the mobility dimension. The platform provides vehicle-to-everything (V2X communication with support for highly reliable and actionable information flows. Moreover, taking into account the high mobility pattern that a smart city scenario presents, we propose and evaluate two forwarding strategies for the opportunistic sensor network.

A Multi-Technology Communication Platform for Urban Mobile Sensing

Science.gov (United States)

Almeida, Rodrigo; Oliveira, Rui

2018-01-01

A common concern in smart cities is the focus on sensing procedures to provide city-wide information to city managers and citizens. To meet the growing demands of smart cities, the network must provide the ability to handle a large number of mobile sensors/devices, with high heterogeneity and unpredictable mobility, by collecting and delivering the sensed information for future treatment. This work proposes a multi-wireless technology communication platform for opportunistic data gathering and data exchange with respect to smart cities. Through the implementation of a proprietary long-range (LoRa) network and an urban sensor network, our platform addresses the heterogeneity of Internet of Things (IoT) devices while conferring communications in an opportunistic manner, increasing the interoperability of our platform. It implements and evaluates a medium access communication (MAC) protocol for LoRa networks with multiple gateways. It also implements mobile Opportunistic VEhicular (mOVE), a delay-tolerant network (DTN)-based architecture to address the mobility dimension. The platform provides vehicle-to-everything (V2X) communication with support for highly reliable and actionable information flows. Moreover, taking into account the high mobility pattern that a smart city scenario presents, we propose and evaluate two forwarding strategies for the opportunistic sensor network. PMID:29649175

iSBatch: a batch-processing platform for data analysis and exploration of live-cell single-molecule microscopy images and other hierarchical datasets.

Science.gov (United States)

Caldas, Victor E A; Punter, Christiaan M; Ghodke, Harshad; Robinson, Andrew; van Oijen, Antoine M

2015-10-01

Recent technical advances have made it possible to visualize single molecules inside live cells. Microscopes with single-molecule sensitivity enable the imaging of low-abundance proteins, allowing for a quantitative characterization of molecular properties. Such data sets contain information on a wide spectrum of important molecular properties, with different aspects highlighted in different imaging strategies. The time-lapsed acquisition of images provides information on protein dynamics over long time scales, giving insight into expression dynamics and localization properties. Rapid burst imaging reveals properties of individual molecules in real-time, informing on their diffusion characteristics, binding dynamics and stoichiometries within complexes. This richness of information, however, adds significant complexity to analysis protocols. In general, large datasets of images must be collected and processed in order to produce statistically robust results and identify rare events. More importantly, as live-cell single-molecule measurements remain on the cutting edge of imaging, few protocols for analysis have been established and thus analysis strategies often need to be explored for each individual scenario. Existing analysis packages are geared towards either single-cell imaging data or in vitro single-molecule data and typically operate with highly specific algorithms developed for particular situations. Our tool, iSBatch, instead allows users to exploit the inherent flexibility of the popular open-source package ImageJ, providing a hierarchical framework in which existing plugins or custom macros may be executed over entire datasets or portions thereof. This strategy affords users freedom to explore new analysis protocols within large imaging datasets, while maintaining hierarchical relationships between experiments, samples, fields of view, cells, and individual molecules.

Article I. Multi-platform Automated Software Building and Packaging

International Nuclear Information System (INIS)

Rodriguez, A Abad; Gomes Gouveia, V E; Meneses, D; Capannini, F; Aimar, A; Di Meglio, A

2012-01-01

One of the major goals of the EMI (European Middleware Initiative) project is the integration of several components of the pre-existing middleware (ARC, gLite, UNICORE and dCache) into a single consistent set of packages with uniform distributions and repositories. Those individual middleware projects have been developed in the last decade by tens of development teams and before EMI were all built and tested using different tools and dedicated services. The software, millions of lines of code, is written in several programming languages and supports multiple platforms. Therefore a viable solution ought to be able to build and test applications on multiple programming languages using common dependencies on all selected platforms. It should, in addition, package the resulting software in formats compatible with the popular Linux distributions, such as Fedora and Debian, and store them in repositories from which all EMI software can be accessed and installed in a uniform way. Despite this highly heterogeneous initial situation, a single common solution, with the aim of quickly automating the integration of the middleware products, had to be selected and implemented in a few months after the beginning of the EMI project. Because of the previous knowledge and the short time available in which to provide this common solution, the ETICS service, where the gLite middleware was already built for years, was selected. This contribution describes how the team in charge of providing a common EMI build and packaging infrastructure to the whole project has developed a homogeneous solution for releasing and packaging the EMI components from the initial set of tools used by the earlier middleware projects. An important element of the presentation is the developers experience and feedback on converging on ETICS and on the on-going work in order to finally add more widely used and supported build and packaging solutions of the Linux platforms

Max-Min SINR in Large-Scale Single-Cell MU-MIMO: Asymptotic Analysis and Low Complexity Transceivers

KAUST Repository

Sifaou, Houssem

2016-12-28

This work focuses on the downlink and uplink of large-scale single-cell MU-MIMO systems in which the base station (BS) endowed with M antennas communicates with K single-antenna user equipments (UEs). Particularly, we aim at reducing the complexity of the linear precoder and receiver that maximize the minimum signal-to-interference-plus-noise ratio subject to a given power constraint. To this end, we consider the asymptotic regime in which M and K grow large with a given ratio. Tools from random matrix theory (RMT) are then used to compute, in closed form, accurate approximations for the parameters of the optimal precoder and receiver, when imperfect channel state information (modeled by the generic Gauss-Markov formulation form) is available at the BS. The asymptotic analysis allows us to derive the asymptotically optimal linear precoder and receiver that are characterized by a lower complexity (due to the dependence on the large scale components of the channel) and, possibly, by a better resilience to imperfect channel state information. However, the implementation of both is still challenging as it requires fast inversions of large matrices in every coherence period. To overcome this issue, we apply the truncated polynomial expansion (TPE) technique to the precoding and receiving vector of each UE and make use of RMT to determine the optimal weighting coefficients on a per- UE basis that asymptotically solve the max-min SINR problem. Numerical results are used to validate the asymptotic analysis in the finite system regime and to show that the proposed TPE transceivers efficiently mimic the optimal ones, while requiring much lower computational complexity.

The Milan Project: A New Method for High-Assurance and High-Performance Computing on Large-Scale Distributed Platforms

National Research Council Canada - National Science Library

Kedem, Zvi

2000-01-01

...: Calypso, Chime, and Charlotte; which enable applications developed for ideal, shared memory, parallel machines to execute on distributed platforms that are subject to failures, slowdowns, and changing resource availability...

Mission Exploitation Platform PROBA-V

Science.gov (United States)

Goor, Erwin

2016-04-01

VITO and partners developed an end-to-end solution to drastically improve the exploitation of the PROBA-V EO-data archive (http://proba-v.vgt.vito.be/), the past mission SPOT-VEGETATION and derived vegetation parameters by researchers, service providers and end-users. The analysis of time series of data (+1PB) is addressed, as well as the large scale on-demand processing of near real-time data. From November 2015 an operational Mission Exploitation Platform (MEP) PROBA-V, as an ESA pathfinder project, will be gradually deployed at the VITO data center with direct access to the complete data archive. Several applications will be released to the users, e.g. - A time series viewer, showing the evolution of PROBA-V bands and derived vegetation parameters for any area of interest. - Full-resolution viewing services for the complete data archive. - On-demand processing chains e.g. for the calculation of N-daily composites. - A Virtual Machine will be provided with access to the data archive and tools to work with this data, e.g. various toolboxes and support for R and Python. After an initial release in January 2016, a research platform will gradually be deployed allowing users to design, debug and test applications on the platform. From the MEP PROBA-V, access to Sentinel-2 and landsat data will be addressed as well, e.g. to support the Cal/Val activities of the users. Users can make use of powerful Web based tools and can self-manage virtual machines to perform their work on the infrastructure at VITO with access to the complete data archive. To realise this, private cloud technology (openStack) is used and a distributed processing environment is built based on Hadoop. The Hadoop ecosystem offers a lot of technologies (Spark, Yarn, Accumulo, etc.) which we integrate with several open-source components. The impact of this MEP on the user community will be high and will completely change the way of working with the data and hence open the large time series to a larger

Adaptation of a web-based, open source electronic medical record system platform to support a large study of tuberculosis epidemiology

Directory of Open Access Journals (Sweden)

Fraser Hamish SF

2012-11-01

Full Text Available Abstract Background In 2006, we were funded by the US National Institutes of Health to implement a study of tuberculosis epidemiology in Peru. The study required a secure information system to manage data from a target goal of 16,000 subjects who needed to be followed for at least one year. With previous experience in the development and deployment of web-based medical record systems for TB treatment in Peru, we chose to use the OpenMRS open source electronic medical record system platform to develop the study information system. Supported by a core technical and management team and a large and growing worldwide community, OpenMRS is now being used in more than 40 developing countries. We adapted the OpenMRS platform to better support foreign languages. We added a new module to support double data entry, linkage to an existing laboratory information system, automatic upload of GPS data from handheld devices, and better security and auditing of data changes. We added new reports for study managers, and developed data extraction tools for research staff and statisticians. Further adaptation to handle direct entry of laboratory data occurred after the study was launched. Results Data collection in the OpenMRS system began in September 2009. By August 2011 a total of 9,256 participants had been enrolled, 102,274 forms and 13,829 laboratory results had been entered, and there were 208 users. The system is now entirely supported by the Peruvian study staff and programmers. Conclusions The information system served the study objectives well despite requiring some significant adaptations mid-stream. OpenMRS has more tools and capabilities than it did in 2008, and requires less adaptations for future projects. OpenMRS can be an effective research data system in resource poor environments, especially for organizations using or considering it for clinical care as well as research.

Platform Expansion Design as Strategic Choice

DEFF Research Database (Denmark)

Staykova, Kalina S.; Damsgaard, Jan

2016-01-01

In this paper, we address how the strategic choice of platform expansion design impacts the subse-quent platform strategy. We identify two distinct approaches to platform expansion – platform bun-dling and platform constellations, which currently co-exist. The purpose of this paper is to outline...

A Typology of Multi-sided Platforms

DEFF Research Database (Denmark)

Staykova, Kalina Stefanova; Damsgaard, Jan

2015-01-01

In this paper we address how the composition of a platform impacts the platform’s business model. By platform’s business model we mean platform features, platform architecture and platform governance. To this end, we construct the Platform Business Model Framework. We apply the framework to three...

A Parallel Approach for Frequent Subgraph Mining in a Single Large Graph Using Spark

Directory of Open Access Journals (Sweden)

Fengcai Qiao

2018-02-01

Full Text Available Frequent subgraph mining (FSM plays an important role in graph mining, attracting a great deal of attention in many areas, such as bioinformatics, web data mining and social networks. In this paper, we propose SSiGraM (Spark based Single Graph Mining, a Spark based parallel frequent subgraph mining algorithm in a single large graph. Aiming to approach the two computational challenges of FSM, we conduct the subgraph extension and support evaluation parallel across all the distributed cluster worker nodes. In addition, we also employ a heuristic search strategy and three novel optimizations: load balancing, pre-search pruning and top-down pruning in the support evaluation process, which significantly improve the performance. Extensive experiments with four different real-world datasets demonstrate that the proposed algorithm outperforms the existing GraMi (Graph Mining algorithm by an order of magnitude for all datasets and can work with a lower support threshold.

Web-based visualization of very large scientific astronomy imagery

Science.gov (United States)

Bertin, E.; Pillay, R.; Marmo, C.

2015-04-01

Visualizing and navigating through large astronomy images from a remote location with current astronomy display tools can be a frustrating experience in terms of speed and ergonomics, especially on mobile devices. In this paper, we present a high performance, versatile and robust client-server system for remote visualization and analysis of extremely large scientific images. Applications of this work include survey image quality control, interactive data query and exploration, citizen science, as well as public outreach. The proposed software is entirely open source and is designed to be generic and applicable to a variety of datasets. It provides access to floating point data at terabyte scales, with the ability to precisely adjust image settings in real-time. The proposed clients are light-weight, platform-independent web applications built on standard HTML5 web technologies and compatible with both touch and mouse-based devices. We put the system to the test and assess the performance of the system and show that a single server can comfortably handle more than a hundred simultaneous users accessing full precision 32 bit astronomy data.

System for beaming power from earth to a high altitude platform

Science.gov (United States)

Friedman, Herbert W.; Porter, Terry J.

2002-01-01

Power is transmitted to a high altitude platform by an array of diode pumped solid state lasers each operated at a single range of laser wavelengths outside of infrared and without using adaptive optics. Each laser produces a beam with a desired arrival spot size. An aircraft avoidance system uses a radar system for automatic control of the shutters of the lasers.

Dynamic Gaming Platform (DGP)

Science.gov (United States)

2009-04-01

GAMING PLATFORM (DGP) Lockheed Martin Corporation...YYYY) APR 09 2. REPORT TYPE Final 3. DATES COVERED (From - To) Jul 07 – Mar 09 4. TITLE AND SUBTITLE DYNAMIC GAMING PLATFORM (DGP) 5a...CMU Carnegie Mellon University DGP Dynamic Gaming Platform GA Genetic Algorithm IARPA Intelligence Advanced Research Projects Activity LM ATL Lockheed Martin Advanced Technology Laboratories PAINT ProActive INTelligence

Large Conization and Laparoendoscopic Single-Port Pelvic Lymphadenectomy in Early-Stage Cervical Cancer for Fertility Preservation

Directory of Open Access Journals (Sweden)

Polat Dursun

2013-01-01

Full Text Available Fertility preservation in early-stage cervical cancer is a hot topic in gynecologic oncology. Although radical vaginal trachelectomy (RVT is suggested as a fertility preserving approach, there are some serious concerns like cervical stenosis, second trimester loss, preterm delivery in survivors, and lack of residual tumor in the majority of the surgical specimens. Therefore, less radical surgical operations have been proposed in early-stage cervical carcinomas. On the other hand, single-incision laparoscopic surgery (SILS is an evolving endoscopic approach for minimal access surgery. In this report, we present a case with early-stage cervical cancer who wishes to preserve fertility. We successfully performed single-port pelvic lymphadenectomy and large conization to preserve fertility potential of the patient. We think that combination of less radical approach like conization and single-port pelvic lymphadenectomy might be less minimally invasive and is still an effective surgical approach in well-selected cases with cervical carcinomas. Incorporation of single-port laparoscopy into the minimally invasive fertility sparing management of the cervical cancer will improve patients outcome with less complications and better cosmesis. Further studies are needed to reach a clear conclusion.

Mobile Work Platform - A Fluor Fernald innovative dismantlement technology

International Nuclear Information System (INIS)

Peters, Mark S.; Cromer, Paul R.; Danner, Robert

2000-01-01

The Department of Energy's (DOE) Office of Science and Technology Decontamination and Decommissioning (D andD) Focus Area, led by the National Energy Technology Laboratory, has been charged with finding new and innovative D and D technologies and then validating through field demonstration that the technologies are safer, faster and/or more cost-effective. The D and D Focus Area's approach to verifying the benefits of the improved D and D technologies is to use them at DOE sites in large-scale demonstration and deployment (LSDD) projects. The DOE's Fernald Environmental Management Project (FEMP), near Cincinnati Ohio, was host for a LSDD Project overseen by the D and D Focus Area. The FEMP was formerly engaged in the production of high quality uranium metal; and is now currently undergoing active environmental restoration, including removal of major process facilities. As observed during the D and D of Fernald's Plant 1, the baseline method for removing piping required laborers to work above the floor on ladders, scaffolding, ardor man-lifts with hand-held power tools. The pipe must first be rigged to prevent falling when cut. After cutting, the pipe is manually lowered to the ground and placed in a storage/disposal container. The Mobile Work Platform (MWP) consists of a mobile chassis, telescoping arm and a dual crimper/shear ''end-effecter''. It has the capability to grab and hold a pipe, crimp and shear the pipe (up to a ten-foot section) on either side of where it is being held and then lower and place the pipe section into a storage/disposal container. The MWP can crimp/shear up to a 6-inch diameter, schedule 401, carbon steel pipe. A single operator using a radio remote control operates the MWP. The paper will describe the results (productivity, safety advantages and lessons learned) during the Mobile Work Platform demonstration at Fernald

Architecture Design of Healthcare Software-as-a-Service Platform for Cloud-Based Clinical Decision Support Service.

Science.gov (United States)

Oh, Sungyoung; Cha, Jieun; Ji, Myungkyu; Kang, Hyekyung; Kim, Seok; Heo, Eunyoung; Han, Jong Soo; Kang, Hyunggoo; Chae, Hoseok; Hwang, Hee; Yoo, Sooyoung

2015-04-01

To design a cloud computing-based Healthcare Software-as-a-Service (SaaS) Platform (HSP) for delivering healthcare information services with low cost, high clinical value, and high usability. We analyzed the architecture requirements of an HSP, including the interface, business services, cloud SaaS, quality attributes, privacy and security, and multi-lingual capacity. For cloud-based SaaS services, we focused on Clinical Decision Service (CDS) content services, basic functional services, and mobile services. Microsoft's Azure cloud computing for Infrastructure-as-a-Service (IaaS) and Platform-as-a-Service (PaaS) was used. The functional and software views of an HSP were designed in a layered architecture. External systems can be interfaced with the HSP using SOAP and REST/JSON. The multi-tenancy model of the HSP was designed as a shared database, with a separate schema for each tenant through a single application, although healthcare data can be physically located on a cloud or in a hospital, depending on regulations. The CDS services were categorized into rule-based services for medications, alert registration services, and knowledge services. We expect that cloud-based HSPs will allow small and mid-sized hospitals, in addition to large-sized hospitals, to adopt information infrastructures and health information technology with low system operation and maintenance costs.

Single Hit Energy-resolved Laue Diffraction

Energy Technology Data Exchange (ETDEWEB)

Patel, Shamim; Suggit, Matthew J.; Stubley, Paul G.; Ciricosta, Orlando; Wark, Justin S.; Higginbotham, Andrew [Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU (United Kingdom); Hawreliak, James A.; Collins, Gilbert W.; Eggert, Jon H. [Lawrence Livermore National Laboratory, Livermore, California 94550 (United States); Comley, Andrew J.; Foster, John M. [Atomic Weapons Establishment, Aldermaston, Reading RG7 4PR (United Kingdom)

2015-05-15

In situ white light Laue diffraction has been successfully used to interrogate the structure of single crystal materials undergoing rapid (nanosecond) dynamic compression up to megabar pressures. However, information on strain state accessible via this technique is limited, reducing its applicability for a range of applications. We present an extension to the existing Laue diffraction platform in which we record the photon energy of a subset of diffraction peaks. This allows for a measurement of the longitudinal and transverse strains in situ during compression. Consequently, we demonstrate measurement of volumetric compression of the unit cell, in addition to the limited aspect ratio information accessible in conventional white light Laue. We present preliminary results for silicon, where only an elastic strain is observed. VISAR measurements show the presence of a two wave structure and measurements show that material downstream of the second wave does not contribute to the observed diffraction peaks, supporting the idea that this material may be highly disordered, or has undergone large scale rotation.

Single Hit Energy-resolved Laue Diffraction

International Nuclear Information System (INIS)

Patel, Shamim; Suggit, Matthew J.; Stubley, Paul G.; Ciricosta, Orlando; Wark, Justin S.; Higginbotham, Andrew; Hawreliak, James A.; Collins, Gilbert W.; Eggert, Jon H.; Comley, Andrew J.; Foster, John M.

2015-01-01

In situ white light Laue diffraction has been successfully used to interrogate the structure of single crystal materials undergoing rapid (nanosecond) dynamic compression up to megabar pressures. However, information on strain state accessible via this technique is limited, reducing its applicability for a range of applications. We present an extension to the existing Laue diffraction platform in which we record the photon energy of a subset of diffraction peaks. This allows for a measurement of the longitudinal and transverse strains in situ during compression. Consequently, we demonstrate measurement of volumetric compression of the unit cell, in addition to the limited aspect ratio information accessible in conventional white light Laue. We present preliminary results for silicon, where only an elastic strain is observed. VISAR measurements show the presence of a two wave structure and measurements show that material downstream of the second wave does not contribute to the observed diffraction peaks, supporting the idea that this material may be highly disordered, or has undergone large scale rotation

NTEGRAL: ICT-platform based Distributed Control in electricity grids with a large share of Distributed Energy Resources and Renewable Energy Sources

OpenAIRE

Peppink , G.; Kamphuis , R.; Kok , K.; Dimeas , A.; Karfopoulos , E.; Hatziargyriou , Nikos D.; Hadjsaid , Nouredine; Caire , Raphaël; Gustavsson , René; Salas , Josep M.; Niesing , Hugo; Van Der Velde , J.; Tena , Llani; Bliek , Frits; Eijgelaar , Marcel

2010-01-01

International audience; The European project INTEGRAL aims to build and demonstrate an industry-quality reference solution for DER aggregation-level control and coordination, based on commonly available ICT components, standards, and platforms. To achieve this, the Integrated ICT-platform based Distributed Control (IIDC) is introduced. The project includes also three field test site installations in the Netherlands, Spain and France, covering normal, critical and emergency grid conditions.

Growing vertical ZnO nanorod arrays within graphite: efficient isolation of large size and high quality single-layer graphene.

Science.gov (United States)

Ding, Ling; E, Yifeng; Fan, Louzhen; Yang, Shihe

2013-07-18

We report a unique strategy for efficiently exfoliating large size and high quality single-layer graphene directly from graphite into DMF dispersions by growing ZnO nanorod arrays between the graphene layers in graphite.

Single Nanowire Probe for Single Cell Endoscopy and Sensing

Science.gov (United States)

Yan, Ruoxue

The ability to manipulate light in subwavelength photonic and plasmonic structures has shown great potentials in revolutionizing how information is generated, transformed and processed. Chemically synthesized nanowires, in particular, offers a unique toolbox not only for highly compact and integrated photonic modules and devices, including coherent and incoherent light sources, waveguides, photodetectors and photovoltaics, but also for new types of nanoscopic bio-probes for spot cargo delivery and in-situ single cell endoscopy and sensing. Such nanowire probes would enable us to carry out intracellular imaging and probing with high spatial resolution, monitor in-vivo biological processes within single living cells and greatly improve our fundamental understanding of cell functions, intracellular physiological processes, and cellular signal pathways. My work is aimed at developing a material and instrumental platform for such single nanowire probe. Successful optical integration of Ag nanowire plasmonic waveguides, which offers deep subwavelength mode confinement, and conventional photonic waveguides was demonstrated on a single nanowire level. The highest plasmonic-photonic coupling efficiency coupling was found at small coupling angles and low input frequencies. The frequency dependent propagation loss was observed in Ag nanowire and was confirmed by quantitative measurement and in agreement with theoretical expectations. Rational integration of dielectric and Ag nanowire waveguide components into hybrid optical-plasmonic routing devices has been demonstrated. This capability is essential for incorporating sub-100nm Ag nanowire waveguides into optical fiber based nanoprobes for single cell endoscopy. The nanoprobe system based on single nanowire waveguides was demonstrated by optically coupling semiconductor or metal nanowire with an optical fiber with tapered tip. This nanoprobe design requires minimal instrumentation which makes it cost efficient and readily

Towards a Disruptive Digital Platform Model

DEFF Research Database (Denmark)

Kazan, Erol

that digital platforms leverage on three strategic design elements (i.e., business, architecture, and technology design) to create supportive conditions for facilitating disruption. To shed light on disruptive digital platforms, I opted for payment platforms as my empirical context and unit of analysis......Digital platforms are layered modular information technology architectures that support disruption. Digital platforms are particularly disruptive, as they facilitate the quick release of digital innovations that may replace established innovations. Yet, despite their support for disruption, we have...... not fully understood how such digital platforms can be strategically designed and configured to facilitate disruption. To that end, this thesis endeavors to unravel disruptive digital platforms from the supply perspective that are grounded on strategic digital platform design elements. I suggest...

Design and Testing of a Thermostable Platform for Multimerization of Single Domain Antibodies

Science.gov (United States)

2012-08-01

H.J. Properties , production, and applications of camelid single domain antibody fragments. Appl. Microbiol. Biot. 2007, 77, 13‒22. 2. Goldman...Conway, J.; Sherwood, L.J.; Fech, M.; Vo, B.; Liu, J.L.; Hayhurst, A. Thermostable llama single domain antibodies for detection of Botulinum A...antiparallel coiled-coil inserted. J. Mol. Bio. 2001, 306, 25‒35. 9. Liu, J.L.; Anderson, G.P.; Goldman, E.R. Isolation of anti- toxin single domain

Groundwater Assessment Platform

OpenAIRE

Podgorski, Joel; Berg, Michael

2018-01-01

The Groundwater Assessment Platform is a free, interactive online GIS platform for the mapping, sharing and statistical modeling of groundwater quality data. The modeling allows users to take advantage of publicly available global datasets of various environmental parameters to produce prediction maps of their contaminant of interest.

Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics.

Science.gov (United States)

Ok, Chi Young; Singh, Rajesh R; Salim, Alaa A

2016-01-01

Tissues are heterogeneous in their components. If cells of interest are a minor population of collected tissue, it would be difficult to obtain genetic or genomic information of the interested cell population with conventional genomic DNA extraction from the collected tissue. Single-cell DNA analysis is important in the analysis of genetics of cell clonality, genetic anticipation, and single-cell DNA polymorphisms. Single-cell PCR using Single Cell Ampligrid/GeXP platform is described in this chapter.

Carcinoma of the large bowel after a single massive dose of radiation in healthy teenagers

International Nuclear Information System (INIS)

Rotmensch, S.; Avigad, I.; Soffer, E.E.; Horowitz, A.; Bar-Meir, S.; Confino, R.; Czerniak, A.; Wolfstein, I.

1986-01-01

Three healthy teenagers were exposed to a single pelvic x-ray irradiation as part of sterilization experiments performed in the Auschwitz concentration camp in 1943. Single and multiple carcinomas of the colon and rectum developed 40 years later in the radiation field. Histologic examination of surgical specimens revealed severe radiation-induced changes in all layers of tumor-adjacent areas. In contrast to previous reports of radiation-induced large bowel cancers, these women had not undergone repeated courses of radiation, had no known co-existing disease that might raise the risk for colonic and rectal malignancies, and had an extremely long and remarkably similar latency period. These cases emphasize the need for long-term surveillance in previously radiated patients. Since thousands of teenagers were subjected to similar sterilization experiments, awareness of this association might help in the early diagnosis of additional cases

Observing the Sun with the Atacama Large Millimeter/submillimeter Array (ALMA): Fast-Scan Single-Dish Mapping

Science.gov (United States)

White, S. M.; Iwai, K.; Phillips, N. M.; Hills, R. E.; Hirota, A.; Yagoubov, P.; Siringo, G.; Shimojo, M.; Bastian, T. S.; Hales, A. S.; Sawada, T.; Asayama, S.; Sugimoto, M.; Marson, R. G.; Kawasaki, W.; Muller, E.; Nakazato, T.; Sugimoto, K.; Brajša, R.; Skokić, I.; Bárta, M.; Kim, S.; Remijan, A. J.; de Gregorio, I.; Corder, S. A.; Hudson, H. S.; Loukitcheva, M.; Chen, B.; De Pontieu, B.; Fleishmann, G. D.; Gary, D. E.; Kobelski, A.; Wedemeyer, S.; Yan, Y.

2017-07-01

The Atacama Large Millimeter/submillimeter Array (ALMA) radio telescope has commenced science observations of the Sun starting in late 2016. Since the Sun is much larger than the field of view of individual ALMA dishes, the ALMA interferometer is unable to measure the background level of solar emission when observing the solar disk. The absolute temperature scale is a critical measurement for much of ALMA solar science, including the understanding of energy transfer through the solar atmosphere, the properties of prominences, and the study of shock heating in the chromosphere. In order to provide an absolute temperature scale, ALMA solar observing will take advantage of the remarkable fast-scanning capabilities of the ALMA 12 m dishes to make single-dish maps of the full Sun. This article reports on the results of an extensive commissioning effort to optimize the mapping procedure, and it describes the nature of the resulting data. Amplitude calibration is discussed in detail: a path that uses the two loads in the ALMA calibration system as well as sky measurements is described and applied to commissioning data. Inspection of a large number of single-dish datasets shows significant variation in the resulting temperatures, and based on the temperature distributions, we derive quiet-Sun values at disk center of 7300 K at λ = 3 mm and 5900 K at λ = 1.3 mm. These values have statistical uncertainties of about 100 K, but systematic uncertainties in the temperature scale that may be significantly larger. Example images are presented from two periods with very different levels of solar activity. At a resolution of about 25'', the 1.3 mm wavelength images show temperatures on the disk that vary over about a 2000 K range. Active regions and plages are among the hotter features, while a large sunspot umbra shows up as a depression, and filament channels are relatively cool. Prominences above the solar limb are a common feature of the single-dish images.

Graphene-Plasmonic Hybrid Platform for Label-Free SERS Biomedical Detection

Science.gov (United States)

Wang, Pu

Surface Enhanced Raman Scattering (SERS) has attracted explosive interest for the wealth of vibrational information it provides with minimal invasive effects to target analyte. Nanotechnology, especially in the form of noble metal nanoparticles exhibit unique electromagnetic and chemical characteristics that are explored to realize ultra-sensitive SERS detection in chemical and biological analysis. Graphene, atom-thick carbon monolayer, exhibits superior chemical stability and bio-compatibility. A combination of SERS-active metal nanostructures and graphene will create various synergies in SERS. The main objective of this research was to exploit the applications of the graphene-Au tip hybrid platform in SERS. The hybrid platform consists of a periodic Au nano-pyramid substrate to provide reproducible plasmonic enhancement, and the superimposed monolayer graphene sheet, serving as "built-in" Raman marker. Extensive theoretical and experimental studies were conducted to determine the potentials of the hybrid platform as SERS substrate. Results from both Finite-Domain Time-Domain (FDTD) numerical simulation and Raman scattering of graphene suggested that the hybrid platform boosted a high density of hotspots yielding 1000 times SERS enhancement of graphene bands. Ultra-high sensitivity of the hybrid platform was demonstrated by bio-molecules including dye, protein and neurotransmitters. Dopamine and serotonin can be detected and distinguished at 10-9 M concentration in the presence of human body fluid. Single molecule detection was obtained using a bi-analyte technique. Graphene supported a vibration mode dependent SERS chemical enhancement of ˜10 to the analyte. Quantitative evaluation of hotspots was presented using spatially resolved Raman mapping of graphene SERS enhancement. Graphene plays a crucial role in quantifying SERS hotspots and paves the path for defining SERS EF that could be universally applied to various SERS systems. A reproducible and statistically

The Logic of Digital Platform Disruption

DEFF Research Database (Denmark)

Kazan, Erol; Tan, Chee-Wee; Lim, Eric T. K.

Digital platforms are disruptive IT artifacts, because they facilitate the quick release of innovative platform derivatives from third parties (e.g., apps). This study endeavours to unravel the disruptive potential, caused by distinct designs and configurations of digital platforms on market...... environments. We postulate that the disruptive potential of digital platforms is determined by the degree of alignment among the business, technology and platform profiles. Furthermore, we argue that the design and configuration of the aforementioned three elements dictates the extent to which open innovation...... is permitted. To shed light on the disruptive potential of digital platforms, we opted for payment platforms as our unit of analysis. Through interviews with experts and payment providers, we seek to gain an in-depth appreciation of how contemporary digital payment platforms are designed and configured...

Transactional Network Platform: Applications

Energy Technology Data Exchange (ETDEWEB)

Katipamula, Srinivas; Lutes, Robert G.; Ngo, Hung; Underhill, Ronald M.

2013-10-31

In FY13, Pacific Northwest National Laboratory (PNNL) with funding from the Department of Energy’s (DOE’s) Building Technologies Office (BTO) designed, prototyped and tested a transactional network platform to support energy, operational and financial transactions between any networked entities (equipment, organizations, buildings, grid, etc.). Initially, in FY13, the concept demonstrated transactions between packaged rooftop air conditioners and heat pump units (RTUs) and the electric grid using applications or "agents" that reside on the platform, on the equipment, on a local building controller or in the Cloud. The transactional network project is a multi-lab effort with Oakridge National Laboratory (ORNL) and Lawrence Berkeley National Laboratory (LBNL) also contributing to the effort. PNNL coordinated the project and also was responsible for the development of the transactional network (TN) platform and three different applications associated with RTUs. This document describes two applications or "agents" in details, and also summarizes the platform. The TN platform details are described in another companion document.

Multi-kW single fiber laser based on an extra large mode area fiber design

Science.gov (United States)

Langner, Andreas; Such, Mario; Schötz, Gerhard; Just, Florian; Leich, Martin; Schwuchow, Anka; Grimm, Stephan; Zimer, Hagen; Kozak, Marcin; Wedel, Björn; Rehmann, Georg; Bachert, Charley; Krause, Volker

2012-02-01

The quality of Yb-doped fused bulk silica produced by sintering of Yb-doped fused silica granulates has improved greatly in the past five years [1 - 4]. In particular, the refractive index and doping level homogeneity of such materials are excellent and we achieved excellent background fiber attenuation of the active core material down to about 20 dB/km at 1200 nm. The improvement of the Yb-doped fused bulk silica has enabled the development of multi-kW fiber laser systems based on a single extra large multimode laser fiber (XLMA fiber). When a single active fiber is used in combination with the XLMA multimode fiber of 1200 μm diameter simple and robust high power fiber laser setups without complex fiber coupling and fiber combiner systems become possible. In this papper, we will discuss in detail the development of the core material based on Yb-doped bulk silica and the characterization of Yb-doped fibers with different core compositions. We will also report on the excellent performance of a 4 kW fiber laser based on a single XLMA-fiber and show the first experimental welding results of steel sheets achieved with such a laser.

E-Learning platform system for the department of library and ...

African Journals Online (AJOL)

The application of e-learning is accentuated amongst higher institutions of learning globally. Due to the increase in enrolment and the daunting task of teaching large classes, there arises the need to adopt e-learning platform to reduce the burden between the teacher and student collaborations. This paper seeks the ...

Vertical Relationships within Platform Marketplaces

Directory of Open Access Journals (Sweden)

Mark J. Tremblay

2016-07-01

Full Text Available In two-sided markets a platform allows consumers and sellers to interact by creating sub-markets within the platform marketplace. For example, Amazon has sub-markets for all of the different product categories available on its site, and smartphones have sub-markets for different types of applications (gaming apps, weather apps, map apps, ridesharing apps, etc.. The network benefits between consumers and sellers depend on the mode of competition within the sub-markets: more competition between sellers lowers product prices, increases the surplus consumers receive from a sub-market, and makes platform membership more desirable for consumers. However, more competition also lowers profits for a seller which makes platform membership less desirable for a seller and reduces seller entry and the number of sub-markets available on the platform marketplace. This dynamic between seller competition within a sub-market and agents’ network benefits leads to platform pricing strategies, participation decisions by consumers and sellers, and welfare results that depend on the mode of competition. Thus, the sub-market structure is important when investigating platform marketplaces.

The vacuum platform

Science.gov (United States)

McNab, A.

2017-10-01

This paper describes GridPP’s Vacuum Platform for managing virtual machines (VMs), which has been used to run production workloads for WLCG and other HEP experiments. The platform provides a uniform interface between VMs and the sites they run at, whether the site is organised as an Infrastructure-as-a-Service cloud system such as OpenStack, or an Infrastructure-as-a-Client system such as Vac. The paper describes our experience in using this platform, in developing and operating VM lifecycle managers Vac and Vcycle, and in interacting with VMs provided by LHCb, ATLAS, ALICE, CMS, and the GridPP DIRAC service to run production workloads.

Design of intelligent power consumption optimization and visualization management platform for large buildings based on internet of things

Directory of Open Access Journals (Sweden)

Gong Shulan

2017-01-01

Full Text Available The buildings provide a significant contribution to total energy consumption and CO2 emission. It has been estimated that the development of an intelligent power consumption monitor and control system will result in about 30% savings in energy consumption. This design innovatively integrates the advanced technologies such as the internet of things, the internet, intelligent buildings and intelligent electricity which can offer open, efficient, convenient energy consumption detection platform in demand side and visual management demonstration application platform in power enterprises side. The system was created to maximize the effective and efficient the use of energy resource. It was development around sensor networks and intelligent gateway and the monitoring center software. This will realize the highly integration and comprehensive application in energy and information to meet the needs with intelligent buildings

GSHR, a Web-Based Platform Provides Gene Set-Level Analyses of Hormone Responses in Arabidopsis

Directory of Open Access Journals (Sweden)

Xiaojuan Ran

2018-01-01

Full Text Available Phytohormones regulate diverse aspects of plant growth and environmental responses. Recent high-throughput technologies have promoted a more comprehensive profiling of genes regulated by different hormones. However, these omics data generally result in large gene lists that make it challenging to interpret the data and extract insights into biological significance. With the rapid accumulation of theses large-scale experiments, especially the transcriptomic data available in public databases, a means of using this information to explore the transcriptional networks is needed. Different platforms have different architectures and designs, and even similar studies using the same platform may obtain data with large variances because of the highly dynamic and flexible effects of plant hormones; this makes it difficult to make comparisons across different studies and platforms. Here, we present a web server providing gene set-level analyses of Arabidopsis thaliana hormone responses. GSHR collected 333 RNA-seq and 1,205 microarray datasets from the Gene Expression Omnibus, characterizing transcriptomic changes in Arabidopsis in response to phytohormones including abscisic acid, auxin, brassinosteroids, cytokinins, ethylene, gibberellins, jasmonic acid, salicylic acid, and strigolactones. These data were further processed and organized into 1,368 gene sets regulated by different hormones or hormone-related factors. By comparing input gene lists to these gene sets, GSHR helped to identify gene sets from the input gene list regulated by different phytohormones or related factors. Together, GSHR links prior information regarding transcriptomic changes induced by hormones and related factors to newly generated data and facilities cross-study and cross-platform comparisons; this helps facilitate the mining of biologically significant information from large-scale datasets. The GSHR is freely available at http://bioinfo.sibs.ac.cn/GSHR/.

On the combination of molecular replacement and single-wavelength anomalous diffraction phasing for automated structure determination

International Nuclear Information System (INIS)

Panjikar, Santosh; Parthasarathy, Venkataraman; Lamzin, Victor S.; Weiss, Manfred S.; Tucker, Paul A.

2009-01-01

The combination of molecular replacement and single-wavelength anomalous diffraction improves the performance of automated structure determination with Auto-Rickshaw. A combination of molecular replacement and single-wavelength anomalous diffraction phasing has been incorporated into the automated structure-determination platform Auto-Rickshaw. The complete MRSAD procedure includes molecular replacement, model refinement, experimental phasing, phase improvement and automated model building. The improvement over the standard SAD or MR approaches is illustrated by ten test cases taken from the JCSG diffraction data-set database. Poor MR or SAD phases with phase errors larger than 70° can be improved using the described procedure and a large fraction of the model can be determined in a purely automatic manner from X-ray data extending to better than 2.6 Å resolution

Scientific data analysis on data-parallel platforms.

Energy Technology Data Exchange (ETDEWEB)

Ulmer, Craig D.; Bayer, Gregory W.; Choe, Yung Ryn; Roe, Diana C.

2010-09-01

As scientific computing users migrate to petaflop platforms that promise to generate multi-terabyte datasets, there is a growing need in the community to be able to embed sophisticated analysis algorithms in the computing platforms' storage systems. Data Warehouse Appliances (DWAs) are attractive for this work, due to their ability to store and process massive datasets efficiently. While DWAs have been utilized effectively in data-mining and informatics applications, they remain largely unproven in scientific workloads. In this paper we present our experiences in adapting two mesh analysis algorithms to function on five different DWA architectures: two Netezza database appliances, an XtremeData dbX database, a LexisNexis DAS, and multiple Hadoop MapReduce clusters. The main contribution of this work is insight into the differences between these DWAs from a user's perspective. In addition, we present performance measurements for ten DWA systems to help understand the impact of different architectural trade-offs in these systems.

[The advantages of implementing an e-learning platform for laparoscopic liver surgery].

Science.gov (United States)

Furcea, L; Graur, F; Scurtu, L; Plitea, N; Pîslă, D; Vaida, C; Deteşan, O; Szilaghy, A; Neagoş, H; Mureşan, A; Vlad, L

2011-01-01

The rapid expansion of laparoscopic surgery has led to the development of training methods for acquiring technical skills. The importance and complexity of laparoscopic liver surgery are arguments for developing a new integrated system of teaching, learning and evaluation, based on modern educational principles, on flexibility allowing wide accessibility among surgeons. This paper presents the development of e-learning platform designed for training in laparoscopic liver surgery and pre-planning of the operation in a virtual environment. E-learning platform makes it possible to simulate laparoscopic liver surgery remotely via internet connection. The addressability of this e-learning platform is large, being represented by young surgeons who are mainly preoccupied by laparoscopic liver surgery, as well as experienced surgeons interested in obtaining a competence in the hepatic minimally invasive surgery.

Tectonic resemblance of the Indian Platform, Pakistan with the Moesian Platform, Romania and strategy for exploration of hydrocarbons

International Nuclear Information System (INIS)

Memon, A.D.

1994-01-01

There is a remarkable tectonic resemblance between the indian Platform (Pakistan) and the Moesian Platform (Romania). As viewed in global tectonic perspective Moeslan and Indian Plates have played important role in Alpine Himalayan Orogeny; Moesian and Indian Platforms are extension of these respective plates. Characteristics features of both the platforms are block faulting which has effected not only the general tectonic framework but has also played important role in oil accumulation. Main producing rocks in the Moesian platform are Jurassic sandstones and cretaceous limestones while in the indian platform cretaceous sandstones are important reservoirs. The average geothermal gradient in the indian platform is 2.45 C/100m with the higher gradients in the central gas producing region. Geothermal gradients in the Moesian platform have an average value of 3 C/100m with higher gradients in the northern in the northern part. Some of the producing structures in both the platforms are remarkably similar, traps associated with normal faults are very important. Extensive exploration carried in the Moesian Platform makes it very important oil producing region of Romania. After the discovery of oil lower Sindh, serious exploration is being carried in the Indian platform. The paper deals with the similarities between these two important platforms. In the light of the studies of the Moesian platform, strategies or exploration of oil and gas in the Indian Platform are suggested. (author)

Utilizing platforms in industrialized construction

DEFF Research Database (Denmark)

Bonev, Martin; Wörösch, Michael; Hvam, Lars

2015-01-01

platform strategies, this researchhighlights key aspects of adapting platform-based developed theory to industrialised construction.Building projects use different layers of product, process and logistics platforms to form the right cost– value ratio for the target market application, while modelling...

Large Scale Automatic Analysis and Classification of Roof Surfaces for the Installation of Solar Panels Using a Multi-Sensor Aerial Platform

Directory of Open Access Journals (Sweden)

Luis López-Fernández

2015-09-01

Full Text Available A low-cost multi-sensor aerial platform, aerial trike, equipped with visible and thermographic sensors is used for the acquisition of all the data needed for the automatic analysis and classification of roof surfaces regarding their suitability to harbor solar panels. The geometry of a georeferenced 3D point cloud generated from visible images using photogrammetric and computer vision algorithms, and the temperatures measured on thermographic images are decisive to evaluate the areas, tilts, orientations and the existence of obstacles to locate the optimal zones inside each roof surface for the installation of solar panels. This information is complemented with the estimation of the solar irradiation received by each surface. This way, large areas may be efficiently analyzed obtaining as final result the optimal locations for the placement of solar panels as well as the information necessary (location, orientation, tilt, area and solar irradiation to estimate the productivity of a solar panel from its technical characteristics.

Dip-Pen Nanolithography on (Bio)Reactive Monolayer and Block-Copolymer Platforms: Deposition of Lines of Single Macromolecules

NARCIS (Netherlands)

Salazar, Ramon B.; Shovsky, A.; Schönherr, Holger; Vancso, Gyula J.

2006-01-01

The application of atomic force microscopy (AFM) tip-mediated molecular transfer (dip-pen nanolithography or DPN) to fabricate nanopatterned (bio)reactive platforms based on dendrimers on reactive self-assembled monolayer (SAM) and polymer thin films is discussed. The transfer of high-molar-mass

Issues in offshore platform research - Part 1: Semi-submersibles

Science.gov (United States)

Sharma, R.; Kim, Tae-Wan; Sha, O. P.; Misra, S. C.

2010-09-01

Availability of economic and efficient energy resources is crucial to a nation's development. Because of their low cost and advancement in drilling and exploration technologies, oil and gas based energy systems are the most widely used energy source throughout the world. The inexpensive oil and gas based energy systems are used for everything, i.e., from transportation of goods and people to the harvesting of crops for food. As the energy demand continues to rise, there is strong need for inexpensive energy solutions. An offshore platform is a large structure that is used to house workers and machinery needed to drill wells in the ocean bed, extract oil and/or natural gas, process the produced fluids, and ship or pipe them to shore. Depending on the circumstances, the offshore platform can be fixed (to the ocean floor) or can consist of an artificial island or can float. Semi-submersibles are used for various purposes in offshore and marine engineering, e.g. crane vessels, drilling vessels, tourist vessels, production platforms and accommodation facilities, etc. The challenges of deepwater drilling have further motivated the researchers to design optimum choices for semi-submersibles for a chosen operating depth. In our series of eight papers, we discuss the design and production aspects of all the types of offshore platforms. In the present part I, we present an introduction and critical analysis of semi-submersibles.

Rancang Bangun Platform Sistem SFN TV Digital DVB-T2

Directory of Open Access Journals (Sweden)

Rakhmat Oktariza

2015-12-01

Full Text Available Menyambut implementasi penuh standar penyiaran TV digital DVB-T2 di Indonesia, pemahaman konsep dan realisasi terkait skema alokasi frekuensi dan susunan pemancar-penerima amat diperlukan untuk dapat melakukan perencanaan jaringan yang matang. Adanya suatu platform untuk implementasi, pengukuran dan analisis kinerja sistem DVB-T2 akan sangat membantu hal tersebut. Tugas Akhir ini bertujuan merancang platform sistem Single Frequency Network (SFN untuk implementasi, pengukuran dan analisis pada aplikasi TV digital berstandar DVB-T2. Rancangan platform untuk pengukuran terdiri atas sebuah PC berisi card DekTec DTA-2131 dan antena UHF sebagai unit penerima, sedangkan dua buah PC berisi card DekTec DTA-2111 sebagai unit pemancar dan sebuah RF channel simulator ditambahkan untuk memungkinkan implementasi dan pengujian SFN secara mandiri dalam skala laboratorium. Pengukuran lapangan melalui drive test dilakukan dengan unit penerima sebagai evaluasi terhadap jaringan penyiaran TV digital di wilayah Gerbang Kertasusila. Hasil yang diperoleh kemudian digunakan dalam simulasi untuk menentukan rekomendasi skenario pengimplementasian SFN di wilayah tersebut, terutama dalam kaitannya dengan faktor Power Imbalance (PI antara dua atau lebih pemancar. Platform yang dirancang sudah mampu diaplikasikan untuk pengukuran dan evaluasi jaringan penyiaran existing. Sementara hasil pengukuran dari 8 test point memberikan rata-rata level RF sebesar -67,70 dBm dan CNR sebesar 20,40 dB untuk wilayah Gerbang Kertasusila. Skenario pasangan pemancar yang direkomendasikan dari hasil simulasi adalah Tx1-2 untuk SFN-SISO dan Tx2-3 untuk SFN-MISO.

Burnout of pulverized biomass particles in large scale boiler - Single particle model approach

Energy Technology Data Exchange (ETDEWEB)

Saastamoinen, Jaakko; Aho, Martti; Moilanen, Antero [VTT Technical Research Centre of Finland, Box 1603, 40101 Jyvaeskylae (Finland); Soerensen, Lasse Holst [ReaTech/ReAddit, Frederiksborgsveij 399, Niels Bohr, DK-4000 Roskilde (Denmark); Clausen, Soennik [Risoe National Laboratory, DK-4000 Roskilde (Denmark); Berg, Mogens [ENERGI E2 A/S, A.C. Meyers Vaenge 9, DK-2450 Copenhagen SV (Denmark)

2010-05-15

Burning of coal and biomass particles are studied and compared by measurements in an entrained flow reactor and by modelling. The results are applied to study the burning of pulverized biomass in a large scale utility boiler originally planned for coal. A simplified single particle approach, where the particle combustion model is coupled with one-dimensional equation of motion of the particle, is applied for the calculation of the burnout in the boiler. The particle size of biomass can be much larger than that of coal to reach complete burnout due to lower density and greater reactivity. The burner location and the trajectories of the particles might be optimised to maximise the residence time and burnout. (author)

Adoption of Mobile Payment Platforms

DEFF Research Database (Denmark)

Staykova, Kalina Stefanova; Damsgaard, Jan

2016-01-01

Numerous mobile payment solutions, which rely on new disruptive technologies, have been launched on the payment market in recent years. But despite the growing number of mobile payment apps, very few solutions have turned to be successful as the majority of them fail to gain a critical mass...... of users. In this paper, we investigate successful platform adoption strategies by using the Reach and Range Framework for Multi-Sided Platforms as a strategic tool to which mobile payment providers can adhere in order to tackle some of the main challenges they face throughout the evolution...... of their platforms. The analysis indicates that successful mobile payment solutions tend to be launched as one-sided platforms and then gradually be expanded into being two-sided. Our study showcases that the success of mobile payment platforms lies with the ability of the platform to balance the reach (number...

SurfaceSlide: a multitouch digital pathology platform.

Directory of Open Access Journals (Sweden)

Yinhai Wang

Full Text Available BACKGROUND: Digital pathology provides a digital environment for the management and interpretation of pathological images and associated data. It is becoming increasing popular to use modern computer based tools and applications in pathological education, tissue based research and clinical diagnosis. Uptake of this new technology is stymied by its single user orientation and its prerequisite and cumbersome combination of mouse and keyboard for navigation and annotation. METHODOLOGY: In this study we developed SurfaceSlide, a dedicated viewing platform which enables the navigation and annotation of gigapixel digitised pathological images using fingertip touch. SurfaceSlide was developed using the Microsoft Surface, a 30 inch multitouch tabletop computing platform. SurfaceSlide users can perform direct panning and zooming operations on digitised slide images. These images are downloaded onto the Microsoft Surface platform from a remote server on-demand. Users can also draw annotations and key in texts using an on-screen virtual keyboard. We also developed a smart caching protocol which caches the surrounding regions of a field of view in multi-resolutions thus providing a smooth and vivid user experience and reducing the delay for image downloading from the internet. We compared the usability of SurfaceSlide against Aperio ImageScope and PathXL online viewer. CONCLUSION: SurfaceSlide is intuitive, fast and easy to use. SurfaceSlide represents the most direct, effective and intimate human-digital slide interaction experience. It is expected that SurfaceSlide will significantly enhance digital pathology tools and applications in education and clinical practice.

The Dynamics of Digital Platform Innovation

DEFF Research Database (Denmark)

Eaton, Ben

2016-01-01

Curated platforms provide an architectural basis for third parties to develop platform complements and for platform owners to control their implementation as a form of open innovation. The refusal to implement complements as innovations can cause tension between platform owners and developers. Th...

Strategies for Sharing Seismic Data Among Multiple Computer Platforms

Science.gov (United States)

Baker, L. M.; Fletcher, J. B.

2001-12-01

Seismic waveform data is readily available from a variety of sources, but it often comes in a distinct, instrument-specific data format. For example, data may be from portable seismographs, such as those made by Refraction Technology or Kinemetrics, from permanent seismograph arrays, such as the USGS Parkfield Dense Array, from public data centers, such as the IRIS Data Center, or from personal communication with other researchers through e-mail or ftp. A computer must be selected to import the data - usually whichever is the most suitable for reading the originating format. However, the computer best suited for a specific analysis may not be the same. When copies of the data are then made for analysis, a proliferation of copies of the same data results, in possibly incompatible, computer-specific formats. In addition, if an error is detected and corrected in one copy, or some other change is made, all the other copies must be updated to preserve their validity. Keeping track of what data is available, where it is located, and which copy is authoritative requires an effort that is easy to neglect. We solve this problem by importing waveform data to a shared network file server that is accessible to all our computers on our campus LAN. We use a Network Appliance file server running Sun's Network File System (NFS) software. Using an NFS client software package on each analysis computer, waveform data can then be read by our MatLab or Fortran applications without first copying the data. Since there is a single copy of the waveform data in a single location, the NFS file system hierarchy provides an implicit complete waveform data catalog and the single copy is inherently authoritative. Another part of our solution is to convert the original data into a blocked-binary format (known historically as USGS DR100 or VFBB format) that is interpreted by MatLab or Fortran library routines available on each computer so that the idiosyncrasies of each machine are not visible to

Laparoendoscopic Single-Site Surgery (LESS for a Large Ovarian Tumour: First Clinical Case Report

Directory of Open Access Journals (Sweden)

Yao Dong Chua

2011-01-01

Main Outcome Measure(s. Conversion to standard laparoscopic technique or laparotomy, estimated blood loss, operative time , extent of scarring, occurrence of intra- and perioperative surgical complications, technical adequacy, and clinical outcome. Result(s. No conversion to standard laparoscopic technique or laparotomy, and no intraoperative or postoperative complications were observed. Total operative time was 99 minutes. The patient was discharged home on postoperative day one. Conclusion(s. Laparoendoscopic single-site bilateral salpingo-oophorectomy of a large ovarian tumour is feasible with standard laparoscopic instruments. It is safe and effective, with good results in terms of excellent cosmesis and minimal postoperative pain.

Expanded Large-Scale Forcing Properties Derived from the Multiscale Data Assimilation System and Its Application to Single-Column Models

Science.gov (United States)

Feng, S.; Li, Z.; Liu, Y.; Lin, W.; Toto, T.; Vogelmann, A. M.; Fridlind, A. M.

2013-12-01

We present an approach to derive large-scale forcing that is used to drive single-column models (SCMs) and cloud resolving models (CRMs)/large eddy simulation (LES) for evaluating fast physics parameterizations in climate models. The forcing fields are derived by use of a newly developed multi-scale data assimilation (MS-DA) system. This DA system is developed on top of the NCEP Gridpoint Statistical Interpolation (GSI) System and is implemented in the Weather Research and Forecasting (WRF) model at a cloud resolving resolution of 2 km. This approach has been applied to the generation of large scale forcing for a set of Intensive Operation Periods (IOPs) over the Atmospheric Radiation Measurement (ARM) Climate Research Facility's Southern Great Plains (SGP) site. The dense ARM in-situ observations and high-resolution satellite data effectively constrain the WRF model. The evaluation shows that the derived forcing displays accuracies comparable to the existing continuous forcing product and, overall, a better dynamic consistency with observed cloud and precipitation. One important application of this approach is to derive large-scale hydrometeor forcing and multiscale forcing, which is not provided in the existing continuous forcing product. It is shown that the hydrometeor forcing poses an appreciable impact on cloud and precipitation fields in the single-column model simulations. The large-scale forcing exhibits a significant dependency on domain-size that represents SCM grid-sizes. Subgrid processes often contribute a significant component to the large-scale forcing, and this contribution is sensitive to the grid-size and cloud-regime.

A Study on SE Methodology for Design of Big Data Pilot Platform to Improve Nuclear Power Plant Safety

Energy Technology Data Exchange (ETDEWEB)

Shin, Junguk; Cha, Jae-Min; Kim, Jun-Young; Park, Sung-Ho; Yeom, Choong-Sub [Institute for Advanced Engineering (IAE), Yongin (Korea, Republic of)

2016-10-15

A big data concept is expected to have a large impact on the safety of the nuclear power plant (NPP) from the beginning of the big data era. Though there are high interests on the NPP safety with the big data, almost no studies on the logical and physical structures and the systematic design methods of the big data platform for the NPP safety have been conducted. For the current study, a new big data pilot platform for the NPP safety is designed with the main focus on the health monitoring and early warning systems, and a tailored design process based on the systems engineering approaches is proposed to manage inherent high complexity of the platform design. The big data concept is expected to have a large impact on the safety of the NPP. So, in this study, the big data pilot platform for the health monitoring and early warning of the NPP is designed. For this, the development process based on the SE approach for the pilot platform is proposed and the design results along with the proposed process are also presented. Implementation of the individual modules and integrations of those are in currently progress.

A Study on SE Methodology for Design of Big Data Pilot Platform to Improve Nuclear Power Plant Safety

International Nuclear Information System (INIS)

Shin, Junguk; Cha, Jae-Min; Kim, Jun-Young; Park, Sung-Ho; Yeom, Choong-Sub

2016-01-01

A big data concept is expected to have a large impact on the safety of the nuclear power plant (NPP) from the beginning of the big data era. Though there are high interests on the NPP safety with the big data, almost no studies on the logical and physical structures and the systematic design methods of the big data platform for the NPP safety have been conducted. For the current study, a new big data pilot platform for the NPP safety is designed with the main focus on the health monitoring and early warning systems, and a tailored design process based on the systems engineering approaches is proposed to manage inherent high complexity of the platform design. The big data concept is expected to have a large impact on the safety of the NPP. So, in this study, the big data pilot platform for the health monitoring and early warning of the NPP is designed. For this, the development process based on the SE approach for the pilot platform is proposed and the design results along with the proposed process are also presented. Implementation of the individual modules and integrations of those are in currently progress

The European Photovoltaic Technology Platform

International Nuclear Information System (INIS)

Nowak, S.; Aulich, H.; Bal, J.L.; Dimmler, B.; Garnier, A.; Jongerden, G.; Luther, J.; Luque, A.; Milner, A.; Nelson, D.; Pataki, I.; Pearsall, N.; Perezagua, E.; Pietruszko, S.; Rehak, J.; Schellekens, E.; Shanker, A.; Silvestrini, G.; Sinke, W.; Willemsen, H.

2006-05-01

The European Photovoltaic Technology Platform is one of the European Technology Platforms, a new instrument proposed by the European Commission. European Technology Platforms (ETPs) are a mechanism to bring together all interested stakeholders to develop a long-term vision to address a specific challenge, create a coherent, dynamic strategy to achieve that vision and steer the implementation of an action plan to deliver agreed programmes of activities and optimise the benefits for all parties. The European Photovoltaic Technology Platform has recently been established to define, support and accompany the implementation of a coherent and comprehensive strategic plan for photovoltaics. The platform will mobilise all stakeholders sharing a long-term European vision for PV, helping to ensure that Europe maintains and improves its industrial position. The platform will realise a European Strategic Research Agenda for PV for the next decade(s). Guided by a Steering Committee of 20 high level decision-makers representing all relevant European PV Stakeholders, the European PV Technology Platform comprises 4 Working Groups dealing with the subjects policy and instruments; market deployment; science, technology and applications as well as developing countries and is supported by a secretariat

Dental Implant Surrounding Marginal Bone Level Evaluation: Platform Switching versus Platform Matching—One-Year Retrospective Study

Directory of Open Access Journals (Sweden)

Eisner Salamanca

2017-01-01

Full Text Available The benefits and feasibility of platform switching have been discussed in several studies, reporting lesser crestal bone loss in platform-switched implants than in platform-matched implants. Objective. The aim of the present study was to observe the changes in vertical and horizontal marginal bone levels in platform-switched and platform-matched dental implants. Materials and Methods. 51 patients received 60 dental implants in the present study over a 1-year period. Measurement was performed between the implant shoulder and the most apical and horizontal marginal defect by periapical radiographs to examine the changes of peri-implant alveolar bone before and 12 months after prosthodontic restoration delivery. Results. These marginal bone measurements showed a bone gain of 0.23±0.58 mm in the vertical gap and 0.22±0.53 mm in the horizontal gap of platform matching, while in platform switching a bone gain of 0.93±1 mm (P<0.05 in the vertical gap and 0.50±0.56 mm in the horizontal gap was found. The average vertical gap reduction from the baseline until 12 months was 0.92±1.11 mm in platform switching and 0.29±0.85 mm in platform matching (P<0.05. Conclusions. Within the limitations of the present study, platform switching seemed to be more effective for a better peri-implant alveolar bone vertical and horizontal gap reduction at 1 year.

Assessment of a nanoparticle bridge platform for molecular electronics measurements

International Nuclear Information System (INIS)

Jafri, S H M; Blom, T; Leifer, K; Stroemme, M; Welch, K; Loefaas, H; Grigoriev, A; Ahuja, R

2010-01-01

A combination of electron beam lithography, photolithography and focused ion beam milling was used to create a nanogap platform, which was bridged by gold nanoparticles in order to make electrical measurements and assess the platform under ambient conditions. Non-functionalized electrodes were tested to determine the intrinsic response of the platform and it was found that creating devices in ambient conditions requires careful cleaning and awareness of the contributions contaminants may make to measurements. The platform was then used to make measurements on octanethiol (OT) and biphenyldithiol (BPDT) molecules by functionalizing the nanoelectrodes with the molecules prior to bridging the nanogap with nanoparticles. Measurements on OT show that it is possible to make measurements on relatively small numbers of molecules, but that a large variation in response can be expected when one of the metal-molecule junctions is physisorbed, which was partially explained by attachment of OT molecules to different sites on the surface of the Au electrode using a density functional theory calculation. On the other hand, when dealing with BPDT, high yields for device creation are very difficult to achieve under ambient conditions. Significant hysteresis in the I-V curves of BPDT was also observed, which was attributed primarily to voltage induced changes at the interface between the molecule and the metal.

Development and evaluation of a high-throughput, low-cost genotyping platform based on oligonucleotide microarrays in rice

Directory of Open Access Journals (Sweden)

Liu Bin

2008-05-01

Full Text Available Abstract Background We report the development of a microarray platform for rapid and cost-effective genetic mapping, and its evaluation using rice as a model. In contrast to methods employing whole-genome tiling microarrays for genotyping, our method is based on low-cost spotted microarray production, focusing only on known polymorphic features. Results We have produced a genotyping microarray for rice, comprising 880 single feature polymorphism (SFP elements derived from insertions/deletions identified by aligning genomic sequences of the japonica cultivar Nipponbare and the indica cultivar 93-11. The SFPs were experimentally verified by hybridization with labeled genomic DNA prepared from the two cultivars. Using the genotyping microarrays, we found high levels of polymorphism across diverse rice accessions, and were able to classify all five subpopulations of rice with high bootstrap support. The microarrays were used for mapping of a gene conferring resistance to Magnaporthe grisea, the causative organism of rice blast disease, by quantitative genotyping of samples from a recombinant inbred line population pooled by phenotype. Conclusion We anticipate this microarray-based genotyping platform, based on its low cost-per-sample, to be particularly useful in applications requiring whole-genome molecular marker coverage across large numbers of individuals.

Microfluidic-Based Multi-Organ Platforms for Drug Discovery

Directory of Open Access Journals (Sweden)

Ahmad Rezaei Kolahchi

2016-09-01

Full Text Available Development of predictive multi-organ models before implementing costly clinical trials is central for screening the toxicity, efficacy, and side effects of new therapeutic agents. Despite significant efforts that have been recently made to develop biomimetic in vitro tissue models, the clinical application of such platforms is still far from reality. Recent advances in physiologically-based pharmacokinetic and pharmacodynamic (PBPK-PD modeling, micro- and nanotechnology, and in silico modeling have enabled single- and multi-organ platforms for investigation of new chemical agents and tissue-tissue interactions. This review provides an overview of the principles of designing microfluidic-based organ-on-chip models for drug testing and highlights current state-of-the-art in developing predictive multi-organ models for studying the cross-talk of interconnected organs. We further discuss the challenges associated with establishing a predictive body-on-chip (BOC model such as the scaling, cell types, the common medium, and principles of the study design for characterizing the interaction of drugs with multiple targets.

The Educational Platform: Constructing Conceptual Frameworks.

Science.gov (United States)

Peca, Kathy; Isham, Mark

2001-01-01

The education faculty at Eastern New Mexico University used educational platforms as a means of developing the unit's conceptual framework. Faculty members developed personal platforms, then synthesized them into one curricular area platform. The resultant unit educational platform became the basis for the unit's conceptual framework, which…

Overview of ESO Large Single Dish Study

Science.gov (United States)

Testi, Leonardo

2018-01-01

In this talk I will briefly summarize the motivation, methodology and outcome of the ESO Submm Single Dish Strategy WG. The WG was established by the ESO Director for Science and completed its work at the end of 2015. I will summarize the status of the report recommendations, which, among other things, led to the organization of the AtLAST workshop.

Information-computational platform for collaborative multidisciplinary investigations of regional climatic changes and their impacts

Science.gov (United States)

Gordov, Evgeny; Lykosov, Vasily; Krupchatnikov, Vladimir; Okladnikov, Igor; Titov, Alexander; Shulgina, Tamara

2013-04-01

Analysis of growing volume of related to climate change data from sensors and model outputs requires collaborative multidisciplinary efforts of researchers. To do it timely and in reliable way one needs in modern information-computational infrastructure supporting integrated studies in the field of environmental sciences. Recently developed experimental software and hardware platform Climate (http://climate.scert.ru/) provides required environment for regional climate change related investigations. The platform combines modern web 2.0 approach, GIS-functionality and capabilities to run climate and meteorological models, process large geophysical datasets and support relevant analysis. It also supports joint software development by distributed research groups, and organization of thematic education for students and post-graduate students. In particular, platform software developed includes dedicated modules for numerical processing of regional and global modeling results for consequent analysis and visualization. Also run of integrated into the platform WRF and «Planet Simulator» models, modeling results data preprocessing and visualization is provided. All functions of the platform are accessible by a user through a web-portal using common graphical web-browser in the form of an interactive graphical user interface which provides, particularly, capabilities of selection of geographical region of interest (pan and zoom), data layers manipulation (order, enable/disable, features extraction) and visualization of results. Platform developed provides users with capabilities of heterogeneous geophysical data analysis, including high-resolution data, and discovering of tendencies in climatic and ecosystem changes in the framework of different multidisciplinary researches. Using it even unskilled user without specific knowledge can perform reliable computational processing and visualization of large meteorological, climatic and satellite monitoring datasets through

UV disinfection of injection and drinking water - an accepted method on offshore oil platforms

International Nuclear Information System (INIS)

Brunner, H.; Klein, H.P.

1985-01-01

Ultraviolet disinfection packages have been developed for the treatment of drinking water and injection water on offshore oil platforms. Large-scale tests with sulphate reducing bacteria out outlined. (Auth.)

National Community Solar Platform

Energy Technology Data Exchange (ETDEWEB)

Rupert, Bart [Clean Energy Collective, Louisville, CO (United States)

2016-06-30

This project was created to provide a National Community Solar Platform (NCSP) portal known as Community Solar Hub, that is available to any entity or individual who wants to develop community solar. This has been done by providing a comprehensive portal to make CEC’s solutions, and other proven community solar solutions, externally available for everyone to access – making the process easy through proven platforms to protect subscribers, developers and utilities. The successful completion of this project provides these tools via a web platform and integration APIs, a wide spectrum of community solar projects included in the platform, multiple groups of customers (utilities, EPCs, and advocates) using the platform to develop community solar, and open access to anyone interested in community solar. CEC’s Incubator project includes web-based informational resources, integrated systems for project information and billing systems, and engagement with customers and users by community solar experts. The combined effort externalizes much of Clean Energy Collective’s industry-leading expertise, allowing third parties to develop community solar without duplicating expensive start-up efforts. The availability of this platform creates community solar projects that are cheaper to build and cheaper to participate in, furthering the goals of DOE’s SunShot Initiative. Final SF 425 Final SF 428 Final DOE F 2050.11 Final Report Narrative

A Novel Platform for Evaluating the Environmental Impacts on Bacterial Cellulose Production.

Science.gov (United States)

Basu, Anindya; Vadanan, Sundaravadanam Vishnu; Lim, Sierin

2018-04-10

Bacterial cellulose (BC) is a biocompatible material with versatile applications. However, its large-scale production is challenged by the limited biological knowledge of the bacteria. The advent of synthetic biology has lead the way to the development of BC producing microbes as a novel chassis. Hence, investigation on optimal growth conditions for BC production and understanding of the fundamental biological processes are imperative. In this study, we report a novel analytical platform that can be used for studying the biology and optimizing growth conditions of cellulose producing bacteria. The platform is based on surface growth pattern of the organism and allows us to confirm that cellulose fibrils produced by the bacteria play a pivotal role towards their chemotaxis. The platform efficiently determines the impacts of different growth conditions on cellulose production and is translatable to static culture conditions. The analytical platform provides a means for fundamental biological studies of bacteria chemotaxis as well as systematic approach towards rational design and development of scalable bioprocessing strategies for industrial production of bacterial cellulose.

A novel test rig to investigate under-platform damper dynamics

Science.gov (United States)

Botto, Daniele; Umer, Muhammad

2018-02-01

In the field of turbomachinery, vibration amplitude is often reduced by dissipating the kinetic energy of the blades with devices that utilize dry friction. Under-platform dampers, for example, are often placed in the underside of two consecutive turbine blades. Dampers are kept in contact with the under-platform of the respective blades by means of the centrifugal force. If the damper is well designed, vibration of blades instigate a relative motion between the under-platform and the damper. A friction force, that is a non-conservative force, arises in the contact and partly dissipates the vibration energy. Several contact models are available in the literature to simulate the contact between the damper and the under-platform. However, the actual dynamics of the blade-damper interaction have not fully understood yet. Several test rigs have been previously developed to experimentally investigate the performance of under-platform dampers. The majority of these experimental setups aim to evaluate the overall damper efficiency in terms of reduction in response amplitude of the blade for a given exciting force that simulates the aerodynamic loads. Unfortunately, the experimental data acquired on the blade dynamics do not provide enough information to understand the damper dynamics. Therefore, the uncertainty on the damper behavior remains a big issue. In this work, a novel experimental test rig has been developed to extensively investigate the damper dynamic behavior. A single replaceable blade is clamped in the rig with a specific clamping device. With this device the blade root is pressed against a groove machined in the test rig. The pushing force is controllable and measurable, to better simulate the actual centrifugal load acting on the blade. Two dampers, one on each side of the blade, are in contact with the blade under-platforms and with platforms on force measuring supports. These supports have been specifically designed to measure the contact forces on the

Flexible experimental FPGA based platform

DEFF Research Database (Denmark)

Andersen, Karsten Holm; Nymand, Morten