WorldWideScience

Sample records for self-potential sp methods

  1. Development of an investigation method for redox condition of rocks by self potential (SP) method

    International Nuclear Information System (INIS)

    Kubota, Kenji; Inohara, Yoshiki; Oyama, Takahiro

    2012-01-01

    One of the major issues in subsurface disposal of low level radioactive wastes is that long term behaviors of sedimentary rocks can be affected by geochemical factors. Redox conditions can affect to corrosion of metal included in artificial barrier or wastes and adsorption characteristics of a nuclide. Therefore, it is necessary to develop a method for evaluating the redox conditions around natural barrier at waste facilities. In general, geochemical properties are acquired by rock samples or water sampling at a borehole. However, there is a possibility not to acquire data we want to evaluate. If geophysical methods are applied, redox conditions can be evaluated widely and briefly. There is a possibility that self potential (SP) and redox conditions have a correlation. So we have conducted self potential method around test caverns where redox front can be observed at the Rokkasho site. The results demonstrated that self potential decreased around redox front. There was a positive correlation between self potential and pH. One of the factors of self potential change is difference of elevation, however, self potential change around redox front was larger than that expected from elevation difference. Zeta potential is one of the important factors for determination of self potential, and it had a correlation with self potential or pH. Therefore, there is a possibility that self potential change occurs by geochemical condition changes around redox front, and redox condition can be detected by self potential method. (author)

  2. The Application of 2-D Resistivity and Self Potential (SP) Methods in Determining the Water Flow

    Science.gov (United States)

    Nordiana, M. M.; Tajudeen Olugbenga, Adeeko; Afiq Saharudin, Muhamad; nabila, S.; El Hidayah Ismail, Noer

    2018-04-01

    Existence of water flow at urban area will decrease the shear strength and increase hydraulic conductivity of soil which finally caused subsurface problems at this area. To avoid landslide, slope instability and disturbance of the ecosystem, good and detailed planning must be done when developing hilly area. The understanding about geological condition has to be considering before construction activities be done. Six 2-D resistivity survey lines with minimum 5 m electrode spacing were executed using Pole-dipole array. The field investigation such as borehole was carried out at multiple locations in the area where the 2-D resistivity method have been conducted. The directions and intensities of the water were evaluated with self-potential (SP) method. Subsequently, the results from borehole were used to verify the results of electrical resistivity method. Interpretation of 2-D resistivity data showed a low resistivity value (ohm-m), which appears to be a zone that is fully saturated with sandy silt and this could be an influence factor the increasing water level because sandy silt is highly permeable in nature. The borehole, support the results of 2-D resistivity method relating a saturated zone in the survey area. There is a good correlation between the 2-D resistivity investigations and the results of borehole records.

  3. Geotechnical Applications of the Self-Potential (SP) Method. Report 2. The Use of Self Potential to Detect Ground-Water Flow in Karst

    Science.gov (United States)

    1989-05-01

    precipitation are major variables influencing SP data. An automated data collection system was devieed enc’used for:G v - taekeso JIA)-long-term...Researchers have used other geophysical techniques (electrical resis- tivity, seismic refraction, gravimetry , terrain conductivity, ground probing...artificial water injections (recharge) into sinkholes, dye trac- ing, and monitoring of soil temperature, precipitation , ground-water conduc- tivity, and

  4. Anomalous self potential (sp) log signatures observed in a water ...

    African Journals Online (AJOL)

    Geophysical logging was done after drilling had been completed in a water well at Okwudor, South Eastern Nigeria. Three electric logs were run viz: Self Potential (SP), Resistivity N16″ and N64″ logs. An anomaly was observed in the SP log. The SP results from this well show some deviation from the standard norm.

  5. Hydrothermal system of the Papandayan Volcano from temperature, self-potential (SP) and geochemical measurements

    Science.gov (United States)

    Byrdina, Svetlana; Revil, André; Gunawan, Hendra; Saing, Ugan B.; Grandis, Hendra

    2017-07-01

    Papandayan volcano in West Java, Indonesia, is characterized by intense hydrothermal activities manifested by numerous fumaroles at three craters or kawah, i.e. Mas, Manuk and Baru. The latter was created after November 2002 phreatic eruption. Since 2011, numerous volcano-tectonic B events are encountered and the volcano was set on alert status on several occasions. The purpose of the present study is to delineate the structure of the summital hydrothermal system from Self-Potential (SP), soil temperature and gas concentrations in the soil (CO2, SO2 and H2S) data. This combination of geophysical and geochemical methods allows identification of the weak permeable zones serving as preferential pathways for hydrothermal circulation and potential candidates to future landslides or flank collapses. This study is an on-going collaborative research project and we plan to conduct electrical resistivity tomography (ERT) and also Induced-Polarization (IP) surveys. Additional data would allow the 3D imaging of the studied area. The IP parameters will be used to characterise and to quantify the degree of alteration of the volcanic rocks as has been shown very recently in the laboratory studies. There are also rocks and soil samples that will undergo laboratory analyses at ISTerre for IP and complex resistivity parameters at the sample scale that will help to interpret the survey results.

  6. Monitoring underground migration of sequestered CO2 using self-potential methods

    Science.gov (United States)

    Ishido, T.; Pritchett, J.; Tosha, T.; Nishi, Y.; Nakanishi, S.

    2013-12-01

    An appropriate monitoring program is indispensable for an individual geologic storage project to aid in answering various operational questions by detecting changes within the reservoir and to provide early warning of potential CO2 leakage through the caprock. Such a program is also essential to reduce uncertainties associated with reservoir parameters and to improve the predictive capability of reservoir models. Repeat geophysical measurements performed at the earth surface show particular promise for monitoring large subsurface volumes. To appraise the utility of geophysical techniques, Ishido et al. carried out numerical simulations of an aquifer system underlying a portion of Tokyo Bay and calculated the temporal changes in geophysical observables caused by changing underground conditions as computed by reservoir simulation (Energy Procedia, 2011). They used 'geophysical postprocessors' to calculate the resulting temporal changes in the earth-surface distributions of microgravity, self-potential (SP), apparent resistivity (from MT surveys) and seismic observables. The applicability of any particular method is likely to be highly site-specific, but these calculations indicate that none of these techniques should be ruled out altogether. Some survey techniques (gravity, MT resistivity) appear to be suitable for characterizing long-term changes, whereas others (seismic reflection, SP) are quite responsive to short term disturbances. The self-potential postprocessor calculates changes in subsurface electrical potential induced by pressure disturbances through electrokinetic coupling (Ishido & Pritchett, JGR 1999). In addition to electrokinetic coupling, SP anomalies may be generated by various other mechanisms such as thermoelectric coupling, electrochemical diffusion potential, etc. In particular, SP anomalies of negative polarity, which are frequently observed near wells, appear to be caused by an underground electrochemical mechanism similar to a galvanic cell

  7. LEAKAGE CHARACTERISTICS OF BASE OF RIVERBANK BY SELF POTENTIAL METHOD AND EXAMINATION OF EFFECTIVENESS OF SELF POTENTIAL METHOD TO HEALTH MONITORING OF BASE OF RIVERBANK

    Science.gov (United States)

    Matsumoto, Kensaku; Okada, Takashi; Takeuchi, Atsuo; Yazawa, Masato; Uchibori, Sumio; Shimizu, Yoshihiko

    Field Measurement of Self Potential Method using Copper Sulfate Electrode was performed in base of riverbank in WATARASE River, where has leakage problem to examine leakage characteristics. Measurement results showed typical S-shape what indicates existence of flow groundwater. The results agreed with measurement results by Ministry of Land, Infrastructure and Transport with good accuracy. Results of 1m depth ground temperature detection and Chain-Array detection showed good agreement with results of the Self Potential Method. Correlation between Self Potential value and groundwater velocity was examined model experiment. The result showed apparent correlation. These results indicate that the Self Potential Method was effective method to examine the characteristics of ground water of base of riverbank in leakage problem.

  8. The Electrical Self-Potential Method as a Non-Intrusive Snow-Hydrological Sensor

    Science.gov (United States)

    Kulessa, B.; Thompson, S. S.; Luethi, M. P.; Essery, R.

    2015-12-01

    Building on growing momentum in the application of geophysical techniques to snow problems and, specifically, on new theory and an electrical geophysical snow hydrological model published recently; we demonstrate for the first time that the electrical self-potential geophysical technique can sense in-situ bulk meltwater fluxes. This has broad and immediate implications for snow measurement practice, modelling and operational snow forecasting. Our ability to measure, quantify and assimilate hydrological properties and processes of snow in operational models is disproportionally poor compared to the significance of seasonal snowmelt as a global water resource and major risk factor in flood and avalanche forecasting. Encouraged by recent theoretical, modelling and laboratory work, we show here that the diurnal evolution of aerially-distributed self-potential magnitudes closely track those of bulk meltwater fluxes in melting in-situ snowpacks at Rhone and Jungfraujoch glaciers, Switzerland. Numerical modelling infers temporally-evolving liquid water contents in the snowpacks on successive days in close agreement with snow-pit measurements. Muting previous concerns, the governing physical and chemical properties of snow and meltwater became temporally invariant for modelling purposes. Because measurement procedure is straightforward and readily automated for continuous monitoring over significant spatial scales, we conclude that the self-potential geophysical method is a highly-promising non-intrusive snow-hydrological sensor for measurement practice, modelling and operational snow forecasting.

  9. Streaming Potential Modeling to Understand the Identification of Hydraulically Active Fractures and Fracture-Matrix Fluid Interactions Using the Self-Potential Method

    Science.gov (United States)

    Jougnot, D.; Roubinet, D.; Linde, N.; Irving, J.

    2016-12-01

    Quantifying fluid flow in fractured media is a critical challenge in a wide variety of research fields and applications. To this end, geophysics offers a variety of tools that can provide important information on subsurface physical properties in a noninvasive manner. Most geophysical techniques infer fluid flow by data or model differencing in time or space (i.e., they are not directly sensitive to flow occurring at the time of the measurements). An exception is the self-potential (SP) method. When water flows in the subsurface, an excess of charge in the pore water that counterbalances electric charges at the mineral-pore water interface gives rise to a streaming current and an associated streaming potential. The latter can be measured with the SP technique, meaning that the method is directly sensitive to fluid flow. Whereas numerous field experiments suggest that the SP method may allow for the detection of hydraulically active fractures, suitable tools for numerically modeling streaming potentials in fractured media do not exist. Here, we present a highly efficient two-dimensional discrete-dual-porosity approach for solving the fluid-flow and associated self-potential problems in fractured domains. Our approach is specifically designed for complex fracture networks that cannot be investigated using standard numerical methods due to computational limitations. We then simulate SP signals associated with pumping conditions for a number of examples to show that (i) accounting for matrix fluid flow is essential for accurate SP modeling and (ii) the sensitivity of SP to hydraulically active fractures is intimately linked with fracture-matrix fluid interactions. This implies that fractures associated with strong SP amplitudes are likely to be hydraulically conductive, attracting fluid flow from the surrounding matrix.

  10. Groundwater exploration by self-potential method; Shizen den`iho ni yoru chikasui tansa

    Energy Technology Data Exchange (ETDEWEB)

    Onaru, I; Irie, S; Mizunaga, H; Ushijima, K [Kyushu University, Fukuoka (Japan). Faculty of Engineering

    1997-05-27

    This paper describes results measured from the field experiments and data analysis of the groundwater exploration by self-potential method. The field survey was conducted in the vicinity of spring water source in the suburbs of Fukuoka City. For the field experiments, potential differences from the standard potential electrode set at the distance about 200 m were measured at multi-points, simultaneously. For the laboratory experiments, assuming that the groundwater flows in a permeable layer, streaming potential change was observed against the changes of flow rate and electrical conductivity. Thus, the generation of streaming potential was investigated. For the experiment using specimens obtained at the spring water source, the potential changed to negative and was stabilized in around -80 mV after 15 minutes. Numerical simulation was conducted by means of the three-dimensional finite difference method using parameters obtained from the laboratory experiments. From these results, the groundwater flow image could be obtained. It was also confirmed that the self-potential observed in the spring water source area was caused by the streaming potential. 11 refs., 9 figs.

  11. The electrical self-potential method is a non-intrusive snow-hydrological sensor

    Science.gov (United States)

    Thompson, S. S.; Kulessa, B.; Essery, R. L. H.; Lüthi, M. P.

    2015-08-01

    Our ability to measure, quantify and assimilate hydrological properties and processes of snow in operational models is disproportionally poor compared to the significance of seasonal snowmelt as a global water resource and major risk factor in flood and avalanche forecasting. Encouraged by recent theoretical, modelling and laboratory work, we show here that the diurnal evolution of aerially-distributed self-potential magnitudes closely track those of bulk meltwater fluxes in melting in-situ snowpacks at Rhone and Jungfraujoch glaciers, Switzerland. Numerical modelling infers temporally-evolving liquid water contents in the snowpacks on successive days in close agreement with snow-pit measurements. Muting previous concerns, the governing physical and chemical properties of snow and meltwater became temporally invariant for modelling purposes. Because measurement procedure is straightforward and readily automated for continuous monitoring over significant spatial scales, we conclude that the self-potential geophysical method is a highly-promising non-intrusive snow-hydrological sensor for measurement practice, modelling and operational snow forecasting.

  12. Application of self-potential method in uranium exploration - a case study from Arbail, Karnataka, India

    International Nuclear Information System (INIS)

    Anantharaman, K.B.; Narasimha Rao, B.; Sethuram, S.; Rao, K.K.

    1986-01-01

    The application of non-radiometric geophysical methods like magnetic, electrical resistivity, induced polarisation, electromagnetic and seismic for uranium exploration has been discussed by many workers. In thispaper it has been demonstrated that the self-potential technique which is simple, fast and cheap can also be effectively and meaningfully employed. For this purpose, a case study from Arbail (Lat 14 0 , 50' 40'', Long 74 0 38' 25''), India where uranium mineralisation is known to occur in association with sulphides, is presented. The method of downward continuation is used to estimate the depth to the top of the target and the results thus obtained are correlated with the data obtained from subsequent borehole drilling. (author)

  13. Efficacy of very fast simulated annealing global optimization method for interpretation of self-potential anomaly by different forward formulation over 2D inclined sheet type structure

    Science.gov (United States)

    Biswas, A.; Sharma, S. P.

    2012-12-01

    Self-Potential anomaly is an important geophysical technique that measures the electrical potential due natural source of current in the Earth's subsurface. An inclined sheet type model is a very familiar structure associated with mineralization, fault plane, groundwater flow and many other geological features which exhibits self potential anomaly. A number of linearized and global inversion approaches have been developed for the interpretation of SP anomaly over different structures for various purposes. Mathematical expression to compute the forward response over a two-dimensional dipping sheet type structures can be described in three different ways using five variables in each case. Complexities in the inversion using three different forward approaches are different. Interpretation of self-potential anomaly using very fast simulated annealing global optimization has been developed in the present study which yielded a new insight about the uncertainty and equivalence in model parameters. Interpretation of the measured data yields the location of the causative body, depth to the top, extension, dip and quality of the causative body. In the present study, a comparative performance of three different forward approaches in the interpretation of self-potential anomaly is performed to assess the efficacy of the each approach in resolving the possible ambiguity. Even though each forward formulation yields the same forward response but optimization of different sets of variable using different forward problems poses different kinds of ambiguity in the interpretation. Performance of the three approaches in optimization has been compared and it is observed that out of three methods, one approach is best and suitable for this kind of study. Our VFSA approach has been tested on synthetic, noisy and field data for three different methods to show the efficacy and suitability of the best method. It is important to use the forward problem in the optimization that yields the

  14. Recent developments in the use of temperature, resistivity and self-potential methods for monitoring embankment dam performance

    Energy Technology Data Exchange (ETDEWEB)

    Sheffer, M.R. [BC Hydro, Vancouver, BC (Canada); Johansson, S.; Sjodahl, P. [HydroResearch AB, Taby (Sweden)

    2009-07-01

    Significant research is being undertaken in the application and development of non-intrusive geophysical techniques as a result of the need for more comprehensive surveillance to detect internal erosion in embankment dams. Seepage and piezometric measurements are the most common methods utilized for dam surveillance. However, the spatial resolution of these measurements is generally not refined enough to detect small, local seepage changes. This paper summarized the current state of the art in the application of temperature, electrical resistivity and self-potential methods to seepage monitoring at embankment dam sites. The paper presented recent developments in using the technique and interpreting seepage parameters for each method. The methods were discussed in the context of both investigation and monitoring applications. It was concluded that the resistivity method is a non-destructive method that is well suited to long-term monitoring and has the ability to cover the entire dam. 25 refs., 11 figs.

  15. Interpretation of self-potential data for dam seepage investigations

    Energy Technology Data Exchange (ETDEWEB)

    Corwin, R.F.; Sheffer, M.R.; Salmon, G. [BC Hydro, Burnaby, BC (Canada)

    2007-04-15

    This book represents one of a series on the subject of geophysical methods and their use in assessing seepage and internal erosion in embankment dams. This manual facilitates the interpretation of self-potential (SP) data generated by subsurface fluid flow, with an emphasis on dam seepage studies. It is intended for users with a background in geophysics or engineering having a general familiarity with both the SP and direct-current (DC) resistivity methods and their applications. It includes an extensive reference list covering all aspects of available SP interpretation techniques, including qualitative, analytical and numerical methods. Particular emphasis is placed on the use of geometric source analytical modeling methods to evaluate SP anomalies. These methods provide a simple yet efficient means of estimating the location and depth of current sources of observed SP data, which may be linked to fluid flow in the subsurface. The manual is primarily oriented toward embankment dams and earthen structures such as levees and dikes. SP methods have been used to investigate seepage through pervious zones and cracks in concrete and concrete-faced structures. The manual describes the nature of SP fields generated by both uniform and non-uniform dam seepage flow, as well as non-seepage sources of SP variations. These methods enable the study of more complex systems and require a more comprehensive analysis of a given field site. refs., tabs., figs.

  16. Correlation of Self Potential and Ground Magnetic Survey Techniques to Investigate Fluid Seepage in Archaeological site, Sungai Batu, Lembah Bujang, Kedah, Malaysia

    OpenAIRE

    Tajudeen O. Adeeko; Nordiana M. Muztaza; Taqiuddin M. Zakaria; Nurina Ismail

    2018-01-01

    One of the substantial of geophysics is to investigate the subsurface condition of the earth (groundwater) using appropriate geophysical techniques. In this research the correlation of self potential (SP) and ground magnetic methods was used to investigate fluid seepage in Archaeological site, Sungai Batu, Lembah Bujang, Kedah, Malaysia. Self-potential method was used to determine flow of water, and Ground magnetic method was used to find object that can influence the result of self potential...

  17. Black hole algorithm for determining model parameter in self-potential data

    Science.gov (United States)

    Sungkono; Warnana, Dwa Desa

    2018-01-01

    Analysis of self-potential (SP) data is increasingly popular in geophysical method due to its relevance in many cases. However, the inversion of SP data is often highly nonlinear. Consequently, local search algorithms commonly based on gradient approaches have often failed to find the global optimum solution in nonlinear problems. Black hole algorithm (BHA) was proposed as a solution to such problems. As the name suggests, the algorithm was constructed based on the black hole phenomena. This paper investigates the application of BHA to solve inversions of field and synthetic self-potential (SP) data. The inversion results show that BHA accurately determines model parameters and model uncertainty. This indicates that BHA is highly potential as an innovative approach for SP data inversion.

  18. Self-potential observations during hydraulic fracturing

    Energy Technology Data Exchange (ETDEWEB)

    Moore, Jeffrey R.; Glaser, Steven D.

    2007-09-13

    The self-potential (SP) response during hydraulic fracturing of intact Sierra granite was investigated in the laboratory. Excellent correlation of pressure drop and SP suggests that the SP response is created primarily by electrokinetic coupling. For low pressures, the variation of SP with pressure drop is linear, indicating a constant coupling coefficient (Cc) of -200 mV/MPa. However for pressure drops >2 MPa, the magnitude of the Cc increases by 80% in an exponential trend. This increasing Cc is related to increasing permeability at high pore pressures caused by dilatancy of micro-cracks, and is explained by a decrease in the hydraulic tortuosity. Resistivity measurements reveal a decrease of 2% prior to hydraulic fracturing and a decrease of {approx}35% after fracturing. An asymmetric spatial SP response created by injectate diffusion into dilatant zones is observed prior to hydraulic fracturing, and in most cases this SP variation revealed the impending crack geometry seconds before failure. At rupture, injectate rushes into the new fracture area where the zeta potential is different than in the rock porosity, and an anomalous SP spike is observed. After fracturing, the spatial SP distribution reveals the direction of fracture propagation. Finally, during tensile cracking in a point load device with no water flow, a SP spike is observed that is caused by contact electrification. However, the time constant of this event is much less than that for transients observed during hydraulic fracturing, suggesting that SP created solely from material fracture does not contribute to the SP response during hydraulic fracturing.

  19. Using self-potential housing technique to model water seepage at the UNHAS housing Antang area

    Science.gov (United States)

    Syahruddin, Muhammad Hamzah

    2017-01-01

    The earth's surface has an electric potential that is known as self-potentiall (SP). One of the causes of the electrical potential at the earth's surface is water seepage into the ground. Electrical potential caused by water velocity seepage into the ground known as streaming potential. How to model water seepage into the ground at the housing Unhas Antang? This study was conducted to answer these questions. The self-potential measurements performed using a simple digital voltmeter Sanwa brand PC500 with a precision of 0.01 mV. While the coordinates of measurements points are self-potential using Global Positioning System. Mmeasurements results thus obtained are plotted using surfer image distribution self-potential housing Unhas Antang. The self-potential data housing Unhas Antang processed by Forward Modeling methods to get a model of water infiltration into the soil. Housing Unhas Antang self-potential has a value of 5 to 23 mV. Self-potential measurements carried out in the rainy season so it can be assumed that the measurement results caused by the velocity water seepage into the ground. The results of modeling the velocity water seepage from the surface to a depth of 3 meters was 2.4 cm/s to 0.2 cm /s. Modeling results showed that the velocity water seepage of the smaller with depth.

  20. A multidisciplinary approach for the study of the effects of active tectonics along the North Anatolian fault zone: possibilities for the application of the electrical self potential method

    Directory of Open Access Journals (Sweden)

    W. Balderer

    1994-06-01

    Full Text Available The aims of this joint interdisciplinary project “Marmara” of ETH Zurich and the Istanbul Technical University (ITO are to study the effects of active tectonics as evidenced by geology, geodesy and seismology on hydrogeology and geothermics in selected areas along the North Anatolian fault zone. Within the framework of this project thermal water systems in seven different areas have been investigated or are under investigation up to now (SchindIer et al., 1993. For three study areas along the North Anatolian fault zone (from east to west of Kuzuluk/Adapazari, Bursa and of Canakkale the investigations with respect to the geological and hydrogeological features are complete. The now possible hydrogeological characterisation shows encouraging possibilities for the application of new methods like the electrical self potential method for the following reasons: 1 a fully interdisciplinary approach, including seismic survey with especially conceived network, geodetic survey to investigate tectonic movements by the GPS method, geothermic survey combined with geological mapping and hydrogeological investigations of normal mineral and thermal waters; 2 groundwaters of very different chemical and isotopical composition e.g.: Ca-HCO3-type thermal waters of up to 82 °C temperature and total mineralisation of 500 mg/I to 1500 mg/I in the Bursa area, Na-HCO3-type cold mineral waters of up to 2500 mg/I to thermal waters of same mineralisation of up to 80 °C temperature, containing large amounts of CO2 of up to 1 l per 1 kg of water (at surface conditions in the Kuzuluk area and Na-Cl-type waters presenting real thermal brines of up to 65 000 mg/I of total mineralisation and temperatures of up to 100 °C in the Canakkale area; 3 distinct types of hydrodynamic flow regime in areas of different geological and tectonic structure. Based on the results of the investigations within these areas the possibilities of further studies including self potential methods

  1. Application of telluric-telluric profiling combined with magnetotelluric and self-potential methods to geothermal exploration in the Fujian Province, China

    Science.gov (United States)

    Pham, Van-Ngoc; Boyer, Danièle; Yuan, Xue Cheng; Liu, Shao Cheng

    1995-05-01

    In the Fujian Province, southeastern China, most of the hot springs emerge in fluviatile valleys and the geothermal resources are mainly medium and low temperature ones by mixing of hot water with cold superficial groundwater. The occurrence of the thermal waters is controlled by deep tectonic fractures in the bedrock where higher-temperature geothermal reservoirs of economic interest are present. The objective of this study is to detect the deeper active hydrothermal zone under a thick sedimentary cover by geoelectrical methods. In the Gui-An site, the combination of telluric-telluric profiling and magnetotelluric methods turns out very efficient to delineate more accurately the width of the deep conductive fracture zone. Moreover, the self-potential method allows us to localize the most active geothermal zone by electrofiltration processes above a convective cell of hot water which flows up from a deep source. The combined results constitute a possible guide for deep geothermal exploration currently encountered in several geothermal regions over the world.

  2. Forward and Inverse Modeling of Self-potential. A Tomography of Groundwater Flow and Comparison Between Deterministic and Stochastic Inversion Methods

    Science.gov (United States)

    Quintero-Chavarria, E.; Ochoa Gutierrez, L. H.

    2016-12-01

    Applications of the Self-potential Method in the fields of Hydrogeology and Environmental Sciences have had significant developments during the last two decades with a strong use on groundwater flows identification. Although only few authors deal with the forward problem's solution -especially in geophysics literature- different inversion procedures are currently being developed but in most cases they are compared with unconventional groundwater velocity fields and restricted to structured meshes. This research solves the forward problem based on the finite element method using the St. Venant's Principle to transform a point dipole, which is the field generated by a single vector, into a distribution of electrical monopoles. Then, two simple aquifer models were generated with specific boundary conditions and head potentials, velocity fields and electric potentials in the medium were computed. With the model's surface electric potential, the inverse problem is solved to retrieve the source of electric potential (vector field associated to groundwater flow) using deterministic and stochastic approaches. The first approach was carried out by implementing a Tikhonov regularization with a stabilized operator adapted to the finite element mesh while for the second a hierarchical Bayesian model based on Markov chain Monte Carlo (McMC) and Markov Random Fields (MRF) was constructed. For all implemented methods, the result between the direct and inverse models was contrasted in two ways: 1) shape and distribution of the vector field, and 2) magnitude's histogram. Finally, it was concluded that inversion procedures are improved when the velocity field's behavior is considered, thus, the deterministic method is more suitable for unconfined aquifers than confined ones. McMC has restricted applications and requires a lot of information (particularly in potentials fields) while MRF has a remarkable response especially when dealing with confined aquifers.

  3. Tomographic analysis of self-potential data in a seismic area of Southern Italy

    Directory of Open Access Journals (Sweden)

    S. Piscitelli

    2000-06-01

    Full Text Available The time and space anomalous behaviour of the Self-Potential (SP field recorded in a seismic area of Southern Apennines, Italy, is discussed. The SP data were collected in the period June 1992-November 1994 along a profile located north of the town of Potenza in the Basilicata region, Italy. The profile is perpendicular to an active fault system, where a W-E directed strike-slip structure has been identified from recent earthquakes. The SP data are modelled using a new tomographic method based on the search for similarities between the observed SP sequence and the surface signature of the electric field due to a scanning point source with unitary positive charge. The point scanner is ideally moved in a vertical cross-section through the profile and a regular 2D matrix of charge occurrence probability values is thus obtained. These values are used to image the state of electric polarization in the subsoil, compatible with the observed SP surface pattern. A selection of 2D tomographies across the profile is then discussed in order to outline the SP source geometry and dynamics within the faulted structure. Finally, the time pattern of the SP polarization state is compared with the local seismicity in the frame of the rock dilatancy-fluid diffusion theory. This comparison allows us to exclude a direct relationship of the SP time behaviour with the seismic sequences which occurred in the area during the SP monitoring period.

  4. Tomographic analysis of self-potential data in a seismic area of Southern Italy

    Energy Technology Data Exchange (ETDEWEB)

    Lapenna, V; Piscitelli, S [Consiglio Nazionale delle Ricerche, Tito, PZ (Italy). Ist. di Metodologie Avanzate di Analisi Ambientale; Patella, D [Naples Univ. Federico II, Naples (Italy). Dipt. di Scienze Fisiche

    2000-04-01

    The time and space anomalous behaviour of the self-potential (SP) field recorded in a seismic area of Southern Apennines (Italy) in the period June 1992-November 1994 are discussed. The SP data are modelled using a new tomographic method based on the search for similarities between the observed SP sequence and the surface signature of the electric field due to a scanning point source with unitary positive charge. The point scanner is ideally moved in a vertical cross-section through the profile and a regular 2D matrix of charge occurrence probability values is thus obtained. These values are used to image the state of electric polarization in the subsoil, compatible with the observed SP surface pattern. A selection of 2D tomographies across the profile is then discussed in order to outline the SP source geometry and dynamics within the faulted structure. Finally, the time pattern of the SP polarization state is compared with the local seismicity in the frame of the rock dilatancy-fluid diffusion theory. This comparison allows to exclude a direct relationship of the SP time behaviour with the seismic sequences which occurred in the area during the SP monitoring period.

  5. Analysis of Self-Potential Response beyond the Fixed Geometry Technique

    Science.gov (United States)

    Mahardika, Harry

    2018-03-01

    The self-potential (SP) method is one of the oldest geophysical methods that are still available for today’s application. Since its early days SP data interpretation has been done qualitatively until the emerging of the fixed geometry analysis that was used to characterize the orientation and the electric-dipole properties of a mineral ore structure. Through the expansion of fundamental theories, computational methods, field-and-lab experiments in the last fifteen years, SP method has emerge from its low-class reputation to become more respectable. It became a complementary package alongside electric-resistivity tomography (ERT) for detecting groundwater flow in the subsurface, and extends to the hydrothermal flow in geothermal areas. As the analysis of SP data becomes more quantitative, its potential applications become more diverse. In this paper, we will show examples of our current SP studies such as the groundwater flow characterization inside a fault area. Lastly we will introduce the application of the "active" SP method - that is the seismoelectric method - which can be used for 4D real-time monitoring systems.

  6. Detecting fluid leakage of a reservoir dam based on streaming self-potential measurements

    Science.gov (United States)

    Song, Seo Young; Kim, Bitnarae; Nam, Myung Jin; Lim, Sung Keun

    2015-04-01

    Between many reservoir dams for agriculture in suburban area of South Korea, water leakage has been reported several times. The dam under consideration in this study, which is located in Gyeong-buk, in the south-east of the Korean Peninsula, was reported to have a large leakage at the right foot of downstream side of the reservoir dam. For the detection of the leakage, not only geological survey but also geophysical explorations have been made for precision safety diagnosis, since the leakage can lead to dam failure. Geophysical exploration includes both electrical-resistivity and self-potential surveys, while geological surveys water permeability test, standard penetration test, and sampling for undisturbed sample during the course of the drilling investigation. The geophysical explorations were made not only along the top of dam but also transverse the heel of dam. The leakage of water installations can change the known-heterogeneous structure of the dam body but also cause streaming spontaneous (self) potential (SP) anomaly, which can be detected by electrical resistivity and SP measurements, respectively. For the interpretation of streaming SP, we used trial-and-error method by comparing synthetic SP data with field SP data for model update. For the computation, we first invert the resistivity data to obtain the distorted resistivity structure of the dam levee then make three-dimensional electrical-resistivity modeling for the streaming potential distribution of the dam levee. Our simulation algorithm of streaming SP distribution based on the integrated finite difference scheme computes two-dimensional (2D) SP distribution based on the distribution of calculated flow velocities of fluid for a given permeability structure together with physical properties. This permeability is repeatedly updated based on error between synthetic and field SP data, until the synthetic data match the field data. Through this trial-and-error-based SP interpretation, we locate the

  7. Extraction methods of Amaranthus sp. grain oil isolation.

    Science.gov (United States)

    Krulj, Jelena; Brlek, Tea; Pezo, Lato; Brkljača, Jovana; Popović, Sanja; Zeković, Zoran; Bodroža Solarov, Marija

    2016-08-01

    Amaranthus sp. is a fast-growing crop with well-known beneficial nutritional values (rich in protein, fat, dietary fiber, ash, and minerals, especially calcium and sodium, and containing a higher amount of lysine than conventional cereals). Amaranthus sp. is an underexploited plant source of squalene, a compound of high importance in the food, cosmetic and pharmaceutical industries. This paper has examined the effects of the different extraction methods (Soxhlet, supercritical fluid and accelerated solvent extraction) on the oil and squalene yield of three genotypes of Amaranthus sp. grain. The highest yield of the extracted oil (78.1 g kg(-1) ) and squalene (4.7 g kg(-1) ) in grain was obtained by accelerated solvent extraction (ASE) in genotype 16. Post hoc Tukey's HSD test at 95% confidence limit showed significant differences between observed samples. Principal component analysis (PCA) and cluster analysis (CA) were used for assessing the effect of different genotypes and extraction methods on oil and squalene yield, and also the fatty acid composition profile. Using coupled PCA and CA of observed samples, possible directions for improving the quality of product can be realized. The results of this study indicate that it is very important to choose both the right genotype and the right method of extraction for optimal oil and squalene yield. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

  8. Evaluation of Süleymanköy (Diyarbakir, Eastern Turkey) and Seferihisar (Izmir, Western Turkey) Self Potential Anomalies with Multilayer Perceptron Neural Networks

    Science.gov (United States)

    Kaftan, Ilknur; Sindirgi, Petek

    2013-04-01

    Self-potential (SP) is one of the oldest geophysical methods that provides important information about near-surface structures. Several methods have been developed to interpret SP data using simple geometries. This study investigated inverse solution of a buried, polarized sphere-shaped self-potential (SP ) anomaly via Multilayer Perceptron Neural Networks ( MLPNN ). The polarization angle ( α ) and depth to the centre of sphere ( h )were estimated. The MLPNN is applied to synthetic and field SP data. In order to see the capability of the method in detecting the number of sources, MLPNN was applied to different spherical models at different depths and locations.. Additionally, the performance of MLPNN was tested by adding random noise to the same synthetic test data. The sphere model successfully obtained similar parameters under different S/N ratios. Then, MLPNN method was applied to two field examples. The first one is the cross section taken from the SP anomaly map of the Ergani-Süleymanköy (Turkey) copper mine. MLPNN was also applied to SP data from Seferihisar Izmir (Western Turkey) geothermal field. The MLPNN results showed good agreement with the original synthetic data set. The effect of The technique gave satisfactory results following the addition of 5% and 10% Gaussian noise levels. The MLPNN results were compared to other SP interpretation techniques, such as Normalized Full Gradient (NFG), inverse solution and nomogram methods. All of the techniques showed strong similarity. Consequently, the synthetic and field applications of this study show that MLPNN provides reliable evaluation of the self potential data modelled by the sphere model.

  9. Characterization of Fractured Reservoirs Using a Combination of Downhole Pressure and Self-Potential Transient Data

    OpenAIRE

    Yuji Nishi; Tsuneo Ishido

    2012-01-01

    In order to appraise the utility of self-potential (SP) measurements to characterize fractured reservoirs, we carried out continuous SP monitoring using multi Ag-AgCl electrodes installed within two open holes at the Kamaishi Mine, Japan. The observed ratio of SP change to pressure change associated with fluid flow showed different behaviors between intact host rock and fractured rock regions. Characteristic behavior peculiar to fractured reservoirs, which is predicted from numerical simulati...

  10. Acceleration and parallelization calculation of EFEN-SP_3 method

    International Nuclear Information System (INIS)

    Yang Wen; Zheng Youqi; Wu Hongchun; Cao Liangzhi; Li Yunzhao

    2013-01-01

    Due to the fact that the exponential function expansion nodal-SP_3 (EFEN-SP_3) method needs further improvement in computational efficiency to routinely carry out PWR whole core pin-by-pin calculation, the coarse mesh acceleration and spatial parallelization were investigated in this paper. The coarse mesh acceleration was built by considering discontinuity factor on each coarse mesh interface and preserving neutron balance within each coarse mesh in space, angle and energy. The spatial parallelization based on MPI was implemented by guaranteeing load balancing and minimizing communications cost to fully take advantage of the modern computing and storage abilities. Numerical results based on a commercial nuclear power reactor demonstrate an speedup ratio of about 40 for the coarse mesh acceleration and a parallel efficiency of higher than 60% with 40 CPUs for the spatial parallelization. With these two improvements, the EFEN code can complete a PWR whole core pin-by-pin calculation with 289 × 289 × 218 meshes and 4 energy groups within 100 s by using 48 CPUs (2.40 GHz frequency). (authors)

  11. Formation mechanism of self-potential at ISL-amenable interlayer oxidation zone sandstone-type uranium deposit and the simulation and application of self-potential anomalies

    International Nuclear Information System (INIS)

    Tang Hongzhi; Liu Qingcheng; Su Zhaofeng; Gong Yuling

    2006-01-01

    Based on the analysis of geochemical characteristics and metallogenic physico-chemical conditions of ISL-amenable sandstone-type uranium deposits, the formation mechanism of self-potential field is discussed, a mathematic calculation model has been set up, and the simulation calculation has been performed for self-potential anomalies above uranium ore bodies of ordinary form, features of survey curve are analysed and methods for correcting topography at self-potential anomalies are discussed, and a simulation curve of self-potential in the area of slope topography has been presented. Finally, the availability of the method is demonstrated by an example. (authors)

  12. Self-potential monitoring of water flux at the HOBE agricultural site, Voulund, Denmark

    Science.gov (United States)

    Jougnot, D.; Linde, N.; Looms, M. C.

    2013-12-01

    The self-potential (SP) method is of interest in hydrology and environmental sciences because of its non-invasive nature and its sensitivity to flow and transport processes in the subsurface. The contribution to the SP signal by water flux is referred to as the streaming potential and is due to the presence of an electrical double layer at the mineral-pore water interface. When water flows through the pore, it gives rise to a streaming current and a resulting measurable electrical voltage between non-polarizable electrodes placed at different locations. This electrokinetic behavior is well understood in water saturated porous media, but the best way to model streaming currents under partial saturation is still under discussion. To better understand SP data within the vadose zone, we conducted field-based monitoring of the vertical distribution of the SP signal following different hydrologic events. The investigations were carried out at the Voulund agricultural test site that is part of the Danish hydrological observatory, HOBE, located in the Skjern river catchment (Denmark) in the middle of a cultivated area. It has been instrumented since 2010 to monitor suction, water content and temperature down to a depth of 3 m, together with meteorological variables and repeated geophysical campaigns (cross borehole electrical resistivity tomography and ground penetrating radar). In July 2011, we installed 15 non-polarizable electrodes at 10 depths within the vadose zone (from 0.25 to 3.10 m) and a reference electrode below the water table (7.30 m). More than 2 years of data acquired at a measurement period of 5 minutes are now available with periods indicative of various hydrologic events, such as natural infiltration, water table rises and a high salinity tracer test. We performed wavelet-based signal analysis and investigated the wavelet coherency of the SP data with other measurement variables. The wavelet coherency analysis displays an anti-correlation between SP and

  13. Modeling of self-potential anomalies near vertical dikes.

    Science.gov (United States)

    Fitterman, D.V.

    1983-01-01

    The self-potential (SP) Green's function for an outcropping vertical dike is derived from solutions for the dc resistivity problem for the same geometry. The Green's functions are numerically integrated over rectangular source regions on the contacts between the dike and the surrounding material to obtain the SP anomaly. The analysis is valid for thermoelectrical source mechanisms. Two types of anomalies can be produced by this geometry. When the two source planes are polarized in opposite directions, a monopolar anomaly is produced. This corresponds to the thermoelectrical properties of the dike being in contrast with the surrounding material. When the thermoelectric coefficients change monotonically across the dike, a dipolar anomaly is produced. In either case positive and negative anomalies are possible, and the greatest variation in potential will occur in the most resistive regions. -Author

  14. Self-potential and Complex Conductivity Monitoring of In Situ Hydrocarbon Remediation in Microbial Fuel Cell

    Science.gov (United States)

    Zhang, C.; Revil, A.; Ren, Z.; Karaoulis, M.; Mendonca, C. A.

    2013-12-01

    Petroleum hydrocarbon contamination of soil and groundwater in both non-aqueous phase liquid and dissolved forms generated from spills and leaks is a wide spread environmental issue. Traditional cleanup of hydrocarbon contamination in soils and ground water using physical, chemical, and biological remedial techniques is often expensive and ineffective. Recent studies show that the microbial fuel cell (MFC) can simultaneously enhance biodegradation of hydrocarbons in soil and groundwater and yield electricity. Non-invasive geophysical techniques such as self-potential (SP) and complex conductivity (induced polarization) have shown the potential to detect and characterize the nature of electron transport mechanism of in situ bioremediation of organic contamination plumes. In this study, we deployed both SP and complex conductivity in lab scale MFCs to monitor time-laps geophysical response of degradation of hydrocarbons by MFC. Two different sizes of MFC reactors were used in this study (DI=15 cm cylinder reactor and 94.5cm x 43.5 cm rectangle reactor), and the initial hydrocarbon concentration is 15 g diesel/kg soil. SP and complex conductivity measurements were measured using non-polarizing Ag/AgCl electrodes. Sensitivity study was also performed using COMSOL Multiphysics to test different electrode configurations. The SP measurements showed stronger anomalies adjacent to the MFC than locations afar, and both real and imaginary parts of complex conductivity are greater in areas close to MFC than areas further away and control samples without MFC. The joint use of SP and complex conductivity could in situ evaluate the dynamic changes of electrochemical parameters during this bioremediation process at spatiotemporal scales unachievable with traditional sampling methods. The joint inversion of these two methods to evaluate the efficiency of MFC enhanced hydrocarbon remediation in the subsurface.

  15. Resistivity and self-potential tomography applied to groundwater remediation and contaminant plumes: Sandbox and field experiments

    Science.gov (United States)

    Mao, D.; Revil, A.; Hort, R. D.; Munakata-Marr, J.; Atekwana, E. A.; Kulessa, B.

    2015-11-01

    Geophysical methods can be used to remotely characterize contaminated sites and monitor in situ enhanced remediation processes. We have conducted one sandbox experiment and one contaminated field investigation to show the robustness of electrical resistivity tomography and self-potential (SP) tomography for these applications. In the sandbox experiment, we injected permanganate in a trichloroethylene (TCE)-contaminated environment under a constant hydraulic gradient. Inverted resistivity tomograms are able to track the evolution of the permanganate plume in agreement with visual observations made on the side of the tank. Self-potential measurements were also performed at the surface of the sandbox using non-polarizing Ag-AgCl electrodes. These data were inverted to obtain the source density distribution with and without the resistivity information. A compact horizontal dipole source located at the front of the plume was obtained from the inversion of these self-potential data. This current dipole may be related to the redox reaction occurring between TCE and permanganate and the strong concentration gradient at the front of the plume. We demonstrate that time-lapse self-potential signals can be used to track the kinetics of an advecting oxidizer plume with acceptable accuracy and, if needed, in real time, but are unable to completely resolve the shape of the plume. In the field investigation, a 3D resistivity tomography is used to characterize an organic contaminant plume (resistive domain) and an overlying zone of solid waste materials (conductive domain). After removing the influence of the streaming potential, the identified source current density had a magnitude of 0.5 A m-2. The strong source current density may be attributed to charge movement between the neighboring zones that encourage abiotic and microbially enhanced reduction and oxidation reactions. In both cases, the self-potential source current density is located in the area of strong resistivity

  16. Implications of Self-Potential Distribution for Groundwater Flow System in a Nonvolcanic Mountain Slope

    OpenAIRE

    Goto, Tada-nori; Kondo, Kazuya; Ito, Rina; Esaki, Keisuke; Oouchi, Yasuo; Abe, Yutaka; Tsujimura, Maki

    2012-01-01

    Self-potential (SP) measurements were conducted at Mt. Tsukuba, Japan, which is a nonvolcanic mountain, to infer groundwater flow system in the mountain. Survey routes were set around the northern slope, and the reliability of observed SP anomaly was checked by using SP values along parallel survey routes; the error was almost within 10 mV. The FFT analysis of the spatial SP distribution allows us a separation of raw data into two components with shorter and longer wavelength. In the shorter ...

  17. Self-potential anomalies in some Italian volcanic areas

    Directory of Open Access Journals (Sweden)

    C. Silenziario

    1996-06-01

    Full Text Available The study of Self-Potential (SP space and time variations in volcanic areas may provide useful information on both the geometrical structure of the volcanic apparatuses and the dynamical behaviour of the feeding and uprising systems. In this paper, the results obtained on the islands of Vulcano (Eolian arc and Ponza (Pontine archipelago and on the Mt. Somma-Vesuvius complex are shown. On the island of Vulcano and on the Mt. Somma-Vesuvius apparatus areal SP surveys were performed with the aim of evidencing anomalies closely associated to the zones of major volcanic activity. On the island of Vulcano a profile across the fumaroles along the crater rim of the Fossa Cone was also carried out in order to have a direct relationship between fumarolic fracture migration and flow rate and SP anomaly space and time variations. The areal survey on the island of Ponza, which is considered an inactive area, is assumed as a reference test with which to compare the amplitude and pattern of the anomalies in the active areas. A tentative interpretation of the SP anomalies in volcanic areas is suggested in terms of electrokinetic phenomena, related to the movement of fluids of both volcanic and non-volcanic origin.

  18. Galvanic interpretation of self-potential signals associated withmicrobial sulfate-reduction

    Energy Technology Data Exchange (ETDEWEB)

    Williams, Kenneth H.; Hubbard, Susan S.; Banfield, Jillian F.

    2007-05-02

    We have evaluated the usefulness of the self-potential (SP)geophysical method to track the onset and location of microbialsulfate-reduction in saturated sediments during organic carbon amendment.Following stimulation of sulfate-reducing bacteria (SRB) by addition oflactate, anomalous voltages exceeding 600 mV correlated in space and timewith the accumulation of dissolved sulfide. Abiotic experiments in whichthe sulfide concentration at the measurement electrode was systematicallyvaried showed a positive correlation between the magnitude of the SPanomaly and differences in the half-cell potential associated with themeasurement and reference electrodes. Thus, we infer that the SPanomaliesresulted from electrochemical differences that developedbetween sulfide-rich regions and areas having higher oxidation potential.In neither experiment did generation of an SP anomaly require thepresence of an in situ electronic conductor, as is required by othermodels. These findings emphasize the importance of incorporation ofelectrochemical effects at electrode surfaces in interpretation of SPdata from geophysical studies. We conclude that SP measurements provide aminimally invasive means for monitoring stimulated sulfate-reductionwithin saturated sediments.

  19. Inversion of self-potential anomalies caused by 2D inclined sheets using neural networks

    International Nuclear Information System (INIS)

    El-Kaliouby, Hesham M; Al-Garni, Mansour A

    2009-01-01

    The modular neural network (MNN) inversion method has been used for inversion of self-potential (SP) data anomalies caused by 2D inclined sheets of infinite horizontal extent. The analysed parameters are the depth (h), the half-width (a), the inclination (α), the zero distance from the origin (x o ) and the polarization amplitude (k). The MNN inversion has been first tested on a synthetic example and then applied to two field examples from the Surda area of Rakha mines, India, and Kalava fault zone, India. The effect of random noise has been studied, and the technique showed satisfactory results. The inversion results show good agreement with the measured field data compared with other inversion techniques in use

  20. Correlation of Self Potential and Ground Magnetic Survey Techniques to Investigate Fluid Seepage in Archaeological site, Sungai Batu, Lembah Bujang, Kedah, Malaysia

    Directory of Open Access Journals (Sweden)

    Tajudeen O. Adeeko

    2018-05-01

    Full Text Available One of the substantial of geophysics is to investigate the subsurface condition of the earth (groundwater using appropriate geophysical techniques. In this research the correlation of self potential (SP and ground magnetic methods was used to investigate fluid seepage in Archaeological site, Sungai Batu, Lembah Bujang, Kedah, Malaysia. Self-potential method was used to determine flow of water, and Ground magnetic method was used to find object that can influence the result of self potential measurement and the aquifer depth, the lines were spread 0m ≤ x ≤ 9m, 0m ≤ y ≤ 30m with a trace intervals of 1.5m and 0.75m per electrode spacing respectively. The result display by Self Potential signals gives a clear understand that water flow from higher value (central towards the lower value which is mostly at the southwest part than other areas and distinct level of feasible flow at different part ranges from -30mV to +35mV,which are very related to seepage flow patterns, negative SP anomalies were related with subsurface seepage flow paths (recharge zone and positive SP anomalies were related with areas of seepage outflow (discharge zone; and Ground Magnetic signals shows good details of the buried materials with high magnetic values which was interpreted as baked clay bricks and low magnetic values indicate groundwater seepage with depth of 5m. Therefore, the two results have correlation significant at 0.8 which show good correlation in groundwater investigation in this study, which validates the results.

  1. The Self-Potential Anomaly Produced by a Subsurface Flow at the Contact of Two Horizontal Layers and Its Quantitative Interpretation

    Directory of Open Access Journals (Sweden)

    Georgios Aim. Skianis

    2012-01-01

    Full Text Available In the present paper the problem of a polarized cylinder with a small cross-section, which is located at the contact of two horizontal layers with different resistivities, is studied. Such a polarization geometry simulates the self-potential (SP field produced by a horizontal flow at the contact between the two layers. First, the expression of the self potential at the space domain is derived, applying the image technique. Then, the expression for the Fourier transform of the SP anomaly is found and the behavior of the amplitude spectrum is studied. Based on this study, a direct interpretation method at the spatial frequency domain is proposed, in order to calculate the depth of the flow and the reflection coefficient of the stratified medium. Experimentation with a synthetic model shows that the method works well (small deviations between true and calculated values. When the SP curve contains noise, deviations between calculated and true depths are smaller than those between calculated and true reflection coefficients. The proposed method, which is also applied on SP data from a geothermal system (Mauri et al., 2010, may be useful in detecting underground water or heat flows.

  2. A least-squares minimisation approach to depth determination from numerical second horizontal self-potential anomalies

    Science.gov (United States)

    Abdelrahman, El-Sayed Mohamed; Soliman, Khalid; Essa, Khalid Sayed; Abo-Ezz, Eid Ragab; El-Araby, Tarek Mohamed

    2009-06-01

    This paper develops a least-squares minimisation approach to determine the depth of a buried structure from numerical second horizontal derivative anomalies obtained from self-potential (SP) data using filters of successive window lengths. The method is based on using a relationship between the depth and a combination of observations at symmetric points with respect to the coordinate of the projection of the centre of the source in the plane of the measurement points with a free parameter (graticule spacing). The problem of depth determination from second derivative SP anomalies has been transformed into the problem of finding a solution to a non-linear equation of the form f(z)=0. Formulas have been derived for horizontal cylinders, spheres, and vertical cylinders. Procedures are also formulated to determine the electric dipole moment and the polarization angle. The proposed method was tested on synthetic noisy and real SP data. In the case of the synthetic data, the least-squares method determined the correct depths of the sources. In the case of practical data (SP anomalies over a sulfide ore deposit, Sariyer, Turkey and over a Malachite Mine, Jefferson County, Colorado, USA), the estimated depths of the buried structures are in good agreement with the results obtained from drilling and surface geology.

  3. Self-potential monitoring of a crude oil contaminated site (Trecate, Italy): first results of the modelling.

    Science.gov (United States)

    Giampaolo, V.; Rizzo, E.; Titov, K.; Maineult, A.; Lapenna, V.

    2012-04-01

    The contamination of soils and groundwater by hydrocarbon, due to blow out, leakage from tank or pipe and oil spill, is a heavy environmental problem because infiltrated oil can persist in the ground for a long time. The existing methods used for the remediation of these contaminated sites are invasive, time consuming and expensive. Therefore, in the last years, there was a growing interest in the use of geophysical methods for environmental monitoring (Atekwana et al., 2000; Chambers et al., 2004; Song et al., 2005; French et al., 2009). A particular attention is given to the self-potential (SP) method because SP is sensitive to the contaminant chemistry and redox processes generated by bacteria during the biodegradation (Atekwana et al., 2004; Naudet and Revil, 2005; Revil et al., 2010). Here we show the results of SP investigations carried out at Trecate site (Italy). This site was affected by a crude oil contamination from a well blowout in 1994. Four SP surveys (October 2009, March 2010, October 2010, and March 2011) were conducted at the site, both in the contaminated and uncontaminated regions. Significant changes are observed between SP data collected at different times. In particular, we found mostly negative electrical potential in October surveys and positive electrical potential in March surveys. The SP distributions can be interpreted as the superposition of many components, including a horizontal water-flow in the saturated shallow aquifer toward South-East, the infiltration movement of water in the unsaturated zone and, possibly, the oxidation-reduction phenomena due to bacterial activity. As the groundwater flow usually produces SP linear trends, the data were detrended by linear regression, taking into account the measured piezometric heads in the aquifer. The detrended SP data show that the SP distribution within the contaminated zone is generally bipolar in October: the southern part of the contaminated area is characterized by negative values

  4. Detecting rapid mass movements using electrical self-potential measurements

    Science.gov (United States)

    Heinze, Thomas; Limbrock, Jonas; Pudasaini, Shiva P.; Kemna, Andreas

    2017-04-01

    Rapid mass movements are a latent danger for lives and infrastructure in almost any part of the world. Often such mass movements are caused by increasing pore pressure, for example, landslides after heavy rainfall or dam breaking after intrusion of water in the dam. Among several other geophysical methods used to observe water movement, the electrical self-potential method has been applied to a broad range of monitoring studies, especially focusing on volcanism and dam leakage but also during hydraulic fracturing and for earthquake prediction. Electrical self-potential signals may be caused by various mechanisms. Though, the most relevant source of the self-potential field in the given context is the streaming potential, caused by a flowing electrolyte through porous media with electrically charged internal surfaces. So far, existing models focus on monitoring water flow in non-deformable porous media. However, as the self-potential is sensitive to hydraulic parameters of the soil, any change in these parameters will cause an alteration of the electric signal. Mass movement will significantly influence the hydraulic parameters of the solid as well as the pressure field, assuming that fluid movement is faster than the pressure diffusion. We will present results of laboratory experiments under drained and undrained conditions with fluid triggered as well as manually triggered mass movements, monitored with self-potential measurements. For the undrained scenarios, we observe a clear correlation between the mass movements and signals in the electric potential, which clearly differ from the underlying potential variations due to increased saturation and fluid flow. In the drained experiments, we do not observe any measurable change in the electric potential. We therefore assume that change in fluid properties and release of the load causes disturbances in flow and streaming potential. We will discuss results of numerical simulations reproducing the observed effect. Our

  5. A new algorithm for gravity or self-potential data interpretation

    International Nuclear Information System (INIS)

    Essa, Khalid S

    2011-01-01

    An inversion algorithm is developed to estimate the depth and the associated model parameters of the anomalous body from the gravity or self-potential (SP) whole measured data. The problem of the depth (z) estimation from the observed data has been transformed into a nonlinear equation of the form F(z) = 0. This equation is then solved for z by minimizing an objective functional in the least-squares sense. Using the estimated depth, the polarization angle and the dipole moment or the depth and the amplitude coefficient are computed from the measured SP or gravity data, respectively. The method is based on determining the root mean square (RMS) of the depths estimated from using all s-values for each shape factor. The minimum RMS is used as a criterion for estimating the correct shape and depth of the buried structure. When the correct shape factor is used, the RMS of the depths is always less than the RMS computed using wrong shape factors. The proposed approach is applicable to a class of geometrically simple anomalous bodies, such as the semi-infinite vertical cylinder, the dike, the horizontal cylinder and the sphere, and it is tested and verified on synthetic examples with and without noise. This technique is also successfully applied to four real datasets for mineral exploration, and it is found that the estimated depths and the associated model parameters are in good agreement with the actual values

  6. Monitoring and modelling of pumping-induced self-potentials for transmissivity estimation within a heterogeneous confined aquifer

    Science.gov (United States)

    DesRoches, Aaron J.; Butler, Karl E.

    2016-12-01

    Variations in self-potentials (SP) measured at surface during pumping of a heterogeneous confined fractured rock aquifer have been monitored and modelled in order to investigate capabilities and limitations of SP methods in estimating aquifer hydraulic properties. SP variations were recorded around a pumping well using an irregular grid of 31 non-polarizing Pb-PbCl2 that were referenced to a remote electrode and connected to a commercial multiplexer and digitizer/data logger through a passive lowpass filter on each channel. The lowpass filter reduced noise by a factor of 10 compared to levels obtained using the data logger's integration-based sampling method for powerline noise suppression alone. SP signals showed a linear relationship with water levels observed in the pumping and monitoring wells over the pumping period, with an apparent electrokinetic coupling coefficient of -3.4 mV · m-1. Following recent developments in SP methodology, variability of the SP response between different electrodes is taken as a proxy for lateral variations in hydraulic head within the aquifer and used to infer lateral variations in the aquifer's apparent transmissivity. In order to demonstrate the viability of this approach, SP is modelled numerically to determine its sensitivity to (i) lateral variations in the hydraulic conductivity of the confined aquifer and (ii) the electrical conductivity of the confining layer and conductive well casing. In all cases, SP simulated on the surface still varies linearly with hydraulic head modelled at the base on the confining layer although the apparent coupling coefficient changes to varying degrees. Using the linear relationship observed in the field, drawdown curves were inferred for each electrode location using SP variations observed over the duration of the pumping period. Transmissivity estimates, obtained by fitting the Theis model to inferred drawdown curves at all 31 electrodes, fell within a narrow range of (2.0-4.2) × 10-3 m2

  7. Implications of Self-Potential Distribution for Groundwater Flow System in a Nonvolcanic Mountain Slope

    Directory of Open Access Journals (Sweden)

    Tada-nori Goto

    2012-01-01

    Full Text Available Self-potential (SP measurements were conducted at Mt. Tsukuba, Japan, which is a nonvolcanic mountain, to infer groundwater flow system in the mountain. Survey routes were set around the northern slope, and the reliability of observed SP anomaly was checked by using SP values along parallel survey routes; the error was almost within 10 mV. The FFT analysis of the spatial SP distribution allows us a separation of raw data into two components with shorter and longer wavelength. In the shorter (altitudinal wavelength than ∼200 meters, several positive SP peaks of more than 100 mV in magnitude are present, which indicate shallow perched water discharges along the slope. In the regional SP pattern of longer wavelength, there are two major perturbations from the general trend reflecting the topographic effect. By comparing the SP and hydrological data, the perturbation around the foothill is interpreted to be caused by heterogeneous infiltration at the ground surface. The perturbation around the summit is also interpreted to be caused by heterogeneous infiltration process, based on a simplified numerical modeling of SP. As a result, the SP pattern is well explained by groundwater flow and infiltration processes. Thus, SP data is thought to be very useful for understanding of groundwater flow system on a mountain scale.

  8. Parameter estimation of Monod model by the Least-Squares method for microalgae Botryococcus Braunii sp

    Science.gov (United States)

    See, J. J.; Jamaian, S. S.; Salleh, R. M.; Nor, M. E.; Aman, F.

    2018-04-01

    This research aims to estimate the parameters of Monod model of microalgae Botryococcus Braunii sp growth by the Least-Squares method. Monod equation is a non-linear equation which can be transformed into a linear equation form and it is solved by implementing the Least-Squares linear regression method. Meanwhile, Gauss-Newton method is an alternative method to solve the non-linear Least-Squares problem with the aim to obtain the parameters value of Monod model by minimizing the sum of square error ( SSE). As the result, the parameters of the Monod model for microalgae Botryococcus Braunii sp can be estimated by the Least-Squares method. However, the estimated parameters value obtained by the non-linear Least-Squares method are more accurate compared to the linear Least-Squares method since the SSE of the non-linear Least-Squares method is less than the linear Least-Squares method.

  9. Transient ElectroMagnetic and Electric Self-Potential survey in the TAG hydrothermal field in MAR

    Science.gov (United States)

    Tao, C.; Deng, X.; Wu, G.; Xi, Z.; Zhou, D.; Zuo, L.

    2012-12-01

    The TAG hydrothermal field is one of the most studied hydrothermal fields. This field covers an area of 5km×5km, which includes low-temperature Mn- and Fe-oxides and nontronites zone, relict massive sulfide mounds as well as active hydrothermal mound(TAG mound) [Thompson, 1985, Rona, 1993]. Drilling program was performed in the ODP (Ocean Drilling Program) Leg 158 in the TAG mound [Humphris, 1996]. In 1996, electrical resistivity survey in the TAG mound was conducted using innovative transient electric dipole-dipole instruments which was carried by DSV 'Alvin' [Cairns et al., 1996, Von Herzen et al., 1996]. In June 2012, the 2nd Leg of the Chinese 26th cruise was carried out in the TAG hydrothermal field at Mid Atlantic Ridge by R/V DAYANGYIHAO. Six TEM (Transient ElectroMagnetic) survey lines were deployed, with four of which across the ODP Leg 158 drilling area. Besides, two SP (Electric Self-Potential) survey lines were across the ODP drilling area. The survey results of TEM preliminary revealed the vertical structure of the TAG hydrothermal field. The survey results of both TEM and SP are consistent with the ODP drilling result, and also agree well with the temperature and water-column anomalies obtained in this leg. Preliminary results show that the TEM and SP methods are capable of revealing the horizontal and vertical distribution of the hydrothermal sulfide fields.

  10. THE PREVALENCE OF Salmonella sp., Listeria sp. AND Aeromonas spp. IN CATFISH (CLARIAS (Clarias gariepinus AND TILAPIA (Tilapia mossambica BY PELLETING METHOD

    Directory of Open Access Journals (Sweden)

    Titik Budiati

    2013-06-01

    Full Text Available The aim of this study was to modify the isolation methods of Aeromonas sp., Salmonella spp., and Listeria sp. in catfish (Clarias gariepinus and tilapia (Tilapia mossambica obtained from wet markets and ponds in Malaysia by pelleting the sample. A total of 108 samples (32 catfish intestines, 32 tilapia intestines, and 44 water samples were obtained from nine wet markets and eight ponds. The modified method was employed by pelleting the samples and followed by either implementing pre-enrichment or without pre-enrichment on the isolation of Salmonella and Listeria spp. The modified method (by pelleting the sample in combination with pre-enrichment was the most efficient for Salmonella and Listeria isolation. The sensitivity of the modified Salmonella isolation method was 0.53 and 0.73 for fish and water samples, respectively. The sensitivity of the modified Listeria method was 1 and 0.92 for fish and water samples, respectively. However, the sensitivity of the method by pelleting the sample was similar to those of non-pelleting the sample on Aeromonas isolation. Five species of Aeromonas spp., seven serovars of Salmonella sp., and four species of Listeria sp. were observed in catfish, tilapia and water samples. Overall, by pelleting the sample offered the beneficial to isolate Aeromonas spp., Salmonella sp. and Listeria spp. in catfish, tilapia and water.

  11. Surface self-potential patterns related to transmissive fracture trends during a water injection test

    Science.gov (United States)

    DesRoches, A. J.; Butler, K. E.; MacQuarrie, K. TB

    2018-03-01

    Variations in self-potential (SP) signals were recorded over an electrode array during a constant head injection test in a fractured bedrock aquifer. Water was injected into a 2.2 m interval isolated between two inflatable packers at 44 m depth in a vertical well. Negative SP responses were recorded on surface corresponding to the start of the injection period with strongest magnitudes recorded in electrodes nearest the well. SP response decreased in magnitude at electrodes further from the well. Deflation of the packer system resulted in a strong reversal in the SP signal. Anomalous SP patterns observed at surface at steady state were found to be aligned with dominant fracture strike orientations found within the test interval. Numerical modelling of fluid and current flow within a simplified fracture network showed that azimuthal patterns in SP are mainly controlled by transmissive fracture orientations. The strongest SP gradients occur parallel to hydraulic gradients associated with water flowing out of the transmissive fractures into the tighter matrix and other less permeable cross-cutting fractures. Sensitivity studies indicate that increasing fracture frequency near the well increases the SP magnitude and enhances the SP anomaly parallel to the transmissive set. Decreasing the length of the transmissive fractures leads to more fluid flow into the matrix and into cross-cutting fractures proximal to the well, resulting in a more circular and higher magnitude SP anomaly. Results from the field experiment and modelling provide evidence that surface-based SP monitoring during constant head injection tests has the ability to identify groundwater flow pathways within a fractured bedrock aquifer.

  12. Application of differential evolution algorithm on self-potential data.

    Science.gov (United States)

    Li, Xiangtao; Yin, Minghao

    2012-01-01

    Differential evolution (DE) is a population based evolutionary algorithm widely used for solving multidimensional global optimization problems over continuous spaces, and has been successfully used to solve several kinds of problems. In this paper, differential evolution is used for quantitative interpretation of self-potential data in geophysics. Six parameters are estimated including the electrical dipole moment, the depth of the source, the distance from the origin, the polarization angle and the regional coefficients. This study considers three kinds of data from Turkey: noise-free data, contaminated synthetic data, and Field example. The differential evolution and the corresponding model parameters are constructed as regards the number of the generations. Then, we show the vibration of the parameters at the vicinity of the low misfit area. Moreover, we show how the frequency distribution of each parameter is related to the number of the DE iteration. Experimental results show the DE can be used for solving the quantitative interpretation of self-potential data efficiently compared with previous methods.

  13. Application of differential evolution algorithm on self-potential data.

    Directory of Open Access Journals (Sweden)

    Xiangtao Li

    Full Text Available Differential evolution (DE is a population based evolutionary algorithm widely used for solving multidimensional global optimization problems over continuous spaces, and has been successfully used to solve several kinds of problems. In this paper, differential evolution is used for quantitative interpretation of self-potential data in geophysics. Six parameters are estimated including the electrical dipole moment, the depth of the source, the distance from the origin, the polarization angle and the regional coefficients. This study considers three kinds of data from Turkey: noise-free data, contaminated synthetic data, and Field example. The differential evolution and the corresponding model parameters are constructed as regards the number of the generations. Then, we show the vibration of the parameters at the vicinity of the low misfit area. Moreover, we show how the frequency distribution of each parameter is related to the number of the DE iteration. Experimental results show the DE can be used for solving the quantitative interpretation of self-potential data efficiently compared with previous methods.

  14. A polygonal nodal SP3 method for whole core Pin-by-Pin neutronics calculation

    Energy Technology Data Exchange (ETDEWEB)

    Li, Yunzhao; Wu, Hongchun; Cao, Liangzhi, E-mail: xjtulyz@gmail.com, E-mail: hongchun@mail.xjtu.edu.cn, E-mail: caolz@mail.xjtu.edu.cn [School of Nuclear Science and Technology, Xi' an Jiaotong University, Shaanxi (China)

    2011-07-01

    In this polygonal nodal-SP3 method, neutron transport equation is transformed by employing an isotropic SP3 method into two coupled equations that are both in the same mathematic form with the diffusion equation, and then a polygonal nodal method is proposed to solve the two coupled equations. In the polygonal nodal method, adjacent nodes are coupled through partial currents, and a nodal response matrix between incoming and outgoing currents is obtained by expanding detailed nodal flux distribution into a sum of exponential functions. This method avoids the transverse integral technique, which is widely used in regular nodal method and can not be used in triangular geometry because of the mathematical singularity. It is demonstrated by the numerical results of the test problems that the k{sub eff} and power distribution agree well with other codes, the triangular nodal-SP3 method appears faster, and that whole core pin-by-pin transport calculation with fine meshes is feasible after parallelization and acceleration. (author)

  15. Joint inversion of hydraulic head and self-potential data associated with harmonic pumping tests

    Science.gov (United States)

    Soueid Ahmed, A.; Jardani, A.; Revil, A.; Dupont, J. P.

    2016-09-01

    Harmonic pumping tests consist in stimulating an aquifer by the means of hydraulic stimulations at some discrete frequencies. The inverse problem consisting in retrieving the hydraulic properties is inherently ill posed and is usually underdetermined when considering the number of well head data available in field conditions. To better constrain this inverse problem, we add self-potential data recorded at the ground surface to the head data. The self-potential method is a passive geophysical method. Its signals are generated by the groundwater flow through an electrokinetic coupling. We showed using a 3-D saturated unconfined synthetic aquifer that the self-potential method significantly improves the results of the harmonic hydraulic tomography. The hydroelectric forward problem is obtained by solving first the Richards equation, describing the groundwater flow, and then using the result in an electrical Poisson equation describing the self-potential problem. The joint inversion problem is solved using a reduction model based on the principal component geostatistical approach. In this method, the large prior covariance matrix is truncated and replaced by its low-rank approximation, allowing thus for notable computational time and storage savings. Three test cases are studied, to assess the validity of our approach. In the first test, we show that when the number of harmonic stimulations is low, combining the harmonic hydraulic and self-potential data does not improve the inversion results. In the second test where enough harmonic stimulations are performed, a significant improvement of the hydraulic parameters is observed. In the last synthetic test, we show that the electrical conductivity field required to invert the self-potential data can be determined with enough accuracy using an electrical resistivity tomography survey using the same electrodes configuration as used for the self-potential investigation.

  16. Improved Method for the Isolation of Biosurfactant Glycolipids from Rhodococcus sp. Strain H13A

    OpenAIRE

    Bryant, Frank O.

    1990-01-01

    An improved method for the isolation of the biosurfactant glycolipids from Rhodococcus sp. strain H13A by using XM 50 diafiltration and isopropanol precipitation was devised. This procedure was advantageous since it removes protein coisolated when the glycolipids are obtained by organic extraction and silicic acid chromatography. The protein apparently does not contribute any biosurfactant characteristics to the glycolipids. The deacylated glycolipid backbone included only a disaccharide.

  17. A rapid technique for estimating the depth and width of a two-dimensional plate from self-potential data

    International Nuclear Information System (INIS)

    Mehanee, Salah; Smith, Paul D; Essa, Khalid S

    2011-01-01

    Rapid techniques for self-potential (SP) data interpretation are of prime importance in engineering and exploration geophysics. Parameters (e.g. depth, width) estimation of the ore bodies has also been of paramount concern in mineral prospecting. In many cases, it is useful to assume that the SP anomaly is due to an ore body of simple geometric shape and to use the data to determine its parameters. In light of this, we describe a rapid approach to determine the depth and horizontal width of a two-dimensional plate from the SP anomaly. The rationale behind the scheme proposed in this paper is that, unlike the two- (2D) and three-dimensional (3D) SP rigorous source current inversions, it does not demand a priori information about the subsurface resistivity distribution nor high computational resources. We apply the second-order moving average operator on the SP anomaly to remove the unwanted (regional) effect, represented by up to a third-order polynomial, using filters of successive window lengths. By defining a function F at a fixed window length (s) in terms of the filtered anomaly computed at two points symmetrically distributed about the origin point of the causative body, the depth (z) corresponding to each half-width (w) is estimated by solving a nonlinear equation in the form ξ(s, w, z) = 0. The estimated depths are then plotted against their corresponding half-widths on a graph representing a continuous curve for this window length. This procedure is then repeated for each available window length. The depth and half-width solution of the buried structure is read at the common intersection of these various curves. The improvement of this method over the published first-order moving average technique for SP data is demonstrated on a synthetic data set. It is then verified on noisy synthetic data, complicated structures and successfully applied to three field examples for mineral exploration and we have found that the estimated depth is in good agreement with

  18. The Self-Potential Anomaly Produced by a Subsurface Flow at the Contact of Two Horizontal Layers and Its Quantitative Interpretation

    OpenAIRE

    Skianis, Georgios Aim.

    2012-01-01

    In the present paper the problem of a polarized cylinder with a small cross-section, which is located at the contact of two horizontal layers with different resistivities, is studied. Such a polarization geometry simulates the self-potential (SP) field produced by a horizontal flow at the contact between the two layers. First, the expression of the self potential at the space domain is derived, applying the image technique. Then, the expression for the Fourier transform of the SP anomaly is f...

  19. Marine self potential and CSEM measurements using an autonomous underwater vehicle

    Science.gov (United States)

    Constable, S.; Kowalczyk, P.; Bloomer, S.

    2017-12-01

    Marine self potential (SP) and controlled source EM (CSEM) measurements are commonly made using instruments towed close to the seafloor, which requires dedicated ship time, is limited to slow speeds, and is subject to navigation errors of 5 to 10 m. An alternative is to mount SP and CSEM sensors on an autonomous underwater vehicle (AUV). We tested this with a pilot study in the Iheya area of the Okinawa Trough, off Japan, using an ISE Explorer-class AUV operated by Fukada Salvage and Marine Works and equipped with a Scripps CSEM receiver system. Parts of this prospect have documented hydrothermal venting and seafloor massive sulfide (SMS) deposits. CSEM signals were generated by deploying battery-powered seafloor transmitters, which emitted 20 amps, alternately every 30 seconds on orthogonal, 10 m antennas. CSEM signals were recorded by 3-axis AC-coupled sensors on the AUV as it flew a pattern 70 m above the seafloor around the transmitters. By transmitting two slightly different frequencies, two or more transmitters can broadcast simultaneously. Measurements were made at the same time using DC-coupled electric field amplifiers, from which self potentials were estimated using regularized inversion, yielding negative anomalies of 10 to 25 mV. Modeling suggests that the anomalies are localized and close to the seafloor. Apparent conductivities as high as 30 S/m were fit to the CSEM data, which strongly suggests that SMS mineralization is associated with the SP anomalies, although it is possible the causative mechanism is at least partly due to hydrothermal venting. In either case, we have demonstrated that AUV-mounted instrument systems are an efficient, effective, and low noise means of collecting marine CSEM and SP data. The entire data set was collected in a single day on station with a 10-hour AUV deployment.

  20. Tracing of paleo-shear zones by self-potential data inversion: case studies from the KTB, Rittsteig, and Grossensees graphite-bearing fault planes

    Science.gov (United States)

    Mehanee, Salah A.

    2015-01-01

    This paper describes a new method for tracing paleo-shear zones of the continental crust by self-potential (SP) data inversion. The method falls within the deterministic inversion framework, and it is exclusively applicable for the interpretation of the SP anomalies measured along a profile over sheet-type structures such as conductive thin films of interconnected graphite precipitations formed on shear planes. The inverse method fits a residual SP anomaly by a single thin sheet and recovers the characteristic parameters (depth to the top h, extension in depth a, amplitude coefficient k, and amount and direction of dip θ) of the sheet. This method minimizes an objective functional in the space of the logarithmed and non-logarithmed model parameters (log( h), log( a), log( k), and θ) successively by the steepest descent (SD) and Gauss-Newton (GN) techniques in order to essentially maintain the stability and convergence of this inverse method. Prior to applying the method to real data, its accuracy, convergence, and stability are successfully verified on numerical examples with and without noise. The method is then applied to SP profiles from the German Continental Deep Drilling Program (Kontinentales Tiefbohrprogramm der Bundesrepublik Deutschla - KTB), Rittsteig, and Grossensees sites in Germany for tracing paleo-shear planes coated with graphitic deposits. The comparisons of geologic sections constructed in this paper (based on the proposed deterministic approach) against the existing published interpretations (obtained based on trial-and-error modeling) for the SP data of the KTB and Rittsteig sites have revealed that the deterministic approach suggests some new details that are of some geological significance. The findings of the proposed inverse scheme are supported by available drilling and other geophysical data. Furthermore, the real SP data of the Grossensees site have been interpreted (apparently for the first time ever) by the deterministic inverse

  1. Self-potential monitoring of a thermal pulse advecting through a preferential flow path

    Science.gov (United States)

    Ikard, S. J.; Revil, A.

    2014-11-01

    There is a need to develop new non-intrusive geophysical methods to detect preferential flow paths in heterogeneous porous media. A laboratory experiment is performed to non-invasively localize a preferential flow pathway in a sandbox using a heat pulse monitored by time-lapse self-potential measurements. Our goal is to investigate the amplitude of the intrinsic thermoelectric self-potential anomalies and the ability of this method to track preferential flow paths. A negative self-potential anomaly (-10 to -15 mV with respect to the background signals) is observed at the surface of the tank after hot water is injected in the upstream reservoir during steady state flow between the upstream and downstream reservoirs of the sandbox. Repeating the same experiment with the same volume of water injected upstream, but at the same temperature as the background pore water, produces a negligible self-potential anomaly. The negative self-potential anomaly is possibly associated with an intrinsic thermoelectric effect, with the temperature dependence of the streaming potential coupling coefficient, or with an apparent thermoelectric effect associated with the temperature dependence of the electrodes themselves. We model the experiment in 3D using a finite element code. Our results show that time-lapse self-potential signals can be used to track the position of traveling heat flow pulses in saturated porous materials, and therefore to find preferential flow pathways, especially in a very permeable environment and in real time. The numerical model and the data allows quantifying the intrinsic thermoelectric coupling coefficient, which is on the order of -0.3 to -1.8 mV per degree Celsius. The temperature dependence of the streaming potential during the experiment is negligible with respect to the intrinsic thermoelectric coupling. However, the temperature dependence of the potential of the electrodes needs to be accounted for and is far from being negligible if the electrodes

  2. Self-Potential Monitoring of Landslides on Field and Laboratory Scale

    Science.gov (United States)

    Heinze, T.; Limbrock, J. K.; Weigand, M.; Wagner, F. M.; Kemna, A.

    2017-12-01

    Among several other geophysical methods used to observe water movement in the ground, the electrical self-potential method has been applied to a broad range of monitoring studies, especially focusing on volcanism and dam leakage but also during hydraulic fracturing. Electrical self-potential signals may be caused by various mechanisms. Though, the most relevant source of the self-potential field in the given context of landslides is the streaming potential, caused by a flowing electrolyte through porous media with electrically charged internal surfaces. So far, existing models focus on monitoring water flow in non-deformable porous media. However, as the self-potential is sensitive to hydraulic parameters of the soil, any change in these parameters will cause an alteration of the electric signal. Mass movement will significantly influence the hydraulic parameters of the solid as well as the pressure field, assuming that fluid movement is faster than pressure diffusion. We present self-potential measurements from over a year of continuous monitoring at an old landslide site. Using a three-dimensional electric-resistivity underground model, the self-potential signal is analyzed with respect to precipitation and the resulting flow in the ground. Additional data from electrical measurements and conventional sensors are included to assess saturation. The field observations are supplemented by laboratory experiments in which we study the behavior of the self-potential during failure of a piled land slope. For the undrained scenarios, we observe a clear correlation between the mass movements and signals in the electric potential, which clearly differ from the underlying potential variations due to increased saturation and fluid flow. In the drained experiments, we do not observe any measurable change in the electric potential. We therefore assume that change in fluid properties and release of the load causes disturbances in flow and streaming potential. Our results

  3. Metode Direct Polymerase Chain Reaction untuk Melacak Campylobacter sp. pada Daging Ayam (DIRECT POLYMERASE CHAIN REACTION METHOD FOR DETECTION CAMPYLOBACTER SP. OF POULTRY MEAT

    Directory of Open Access Journals (Sweden)

    Andriani .

    2013-08-01

    Full Text Available Campylobacter sp. is the most commonly reported as agent of foodborne zoonosis causing acutegastroenteritis in humans. Poultry meat is considered as a major source of C. jejuni infection in human.The conventional methods for detecting foodborne bacteria is time-consuming which rely on the of thebacteria in culture media, followed by biochemical identification. In this study polymerase chain reaction(PCR technique was used for rapid identification of the pathogenic Campylobacter sp. The samples usedwere 298 chicken carcass with sold in supermarkets and traditional markets, and were carried out inaccordance the isolation protocol ISO/ DIS 10272-1994. Identification was performed using biochemicalAPI Campy. The direct PCR (DPCR assay with two sets of primers was employed for isolation andidentification of C. jejuni and C. coli. The result of the isolation and identification both by conventional orPCR methods showed that chicken carcasses both from supermarket and traditional market werecontaminated with C. jejuni and or C. coli. Prevalence of Campylobacter sp. contamination in chicken meatwas higher by DPCR (62.6% than by conventional (19.8%, indicating that DPCR technique was moresensitive than conventional method with detection limit for C. jejuni was103 cfu/ml.

  4. Unscented Kalman filter assimilation of time-lapse self-potential data for monitoring solute transport

    Science.gov (United States)

    Cui, Yi-an; Liu, Lanbo; Zhu, Xiaoxiong

    2017-08-01

    Monitoring the extent and evolution of contaminant plumes in local and regional groundwater systems from existing landfills is critical in contamination control and remediation. The self-potential survey is an efficient and economical nondestructive geophysical technique that can be used to investigate underground contaminant plumes. Based on the unscented transform, we have built a Kalman filtering cycle to conduct time-lapse data assimilation for monitoring the transport of solute based on the solute transport experiment using a bench-scale physical model. The data assimilation was formed by modeling the evolution based on the random walk model and observation correcting based on the self-potential forward. Thus, monitoring self-potential data can be inverted by the data assimilation technique. As a result, we can reconstruct the dynamic process of the contaminant plume instead of using traditional frame-to-frame static inversion, which may cause inversion artifacts. The data assimilation inversion algorithm was evaluated through noise-added synthetic time-lapse self-potential data. The result of the numerical experiment shows validity, accuracy and tolerance to the noise of the dynamic inversion. To validate the proposed algorithm, we conducted a scaled-down sandbox self-potential observation experiment to generate time-lapse data that closely mimics the real-world contaminant monitoring setup. The results of physical experiments support the idea that the data assimilation method is a potentially useful approach for characterizing the transport of contamination plumes using the unscented Kalman filter (UKF) data assimilation technique applied to field time-lapse self-potential data.

  5. Characterization of Fractured Reservoirs Using a Combination of Downhole Pressure and Self-Potential Transient Data

    Directory of Open Access Journals (Sweden)

    Yuji Nishi

    2012-01-01

    Full Text Available In order to appraise the utility of self-potential (SP measurements to characterize fractured reservoirs, we carried out continuous SP monitoring using multi Ag-AgCl electrodes installed within two open holes at the Kamaishi Mine, Japan. The observed ratio of SP change to pressure change associated with fluid flow showed different behaviors between intact host rock and fractured rock regions. Characteristic behavior peculiar to fractured reservoirs, which is predicted from numerical simulations of electrokinetic phenomena in MINC (multiple interacting continua double-porosity media, was observed near the fractures. Semilog plots of the ratio of SP change to pressure change observed in one of the two wells show obvious transition from intermediate time increasing to late time stable trends, which indicate that the time required for pressure equilibration between the fracture and matrix regions is about 800 seconds. Fracture spacing was estimated to be a few meters assuming several micro-darcies (10-18 m2 of the matrix region permeability, which is consistent with geological and hydrological observations.

  6. KIN SP: A boundary element method based code for single pile kinematic bending in layered soil

    Directory of Open Access Journals (Sweden)

    Stefano Stacul

    2018-02-01

    Full Text Available In high seismicity areas, it is important to consider kinematic effects to properly design pile foundations. Kinematic effects are due to the interaction between pile and soil deformations induced by seismic waves. One of the effect is the arise of significant strains in weak soils that induce bending moments on piles. These moments can be significant in presence of a high stiffness contrast in a soil deposit. The single pile kinematic interaction problem is generally solved with beam on dynamic Winkler foundation approaches (BDWF or using continuous models. In this work, a new boundary element method (BEM based computer code (KIN SP is presented where the kinematic analysis is preceded by a free-field response analysis. The analysis results of this method, in terms of bending moments at the pile-head and at the interface of a two-layered soil, are influenced by many factors including the soil–pile interface discretization. A parametric study is presented with the aim to suggest the minimum number of boundary elements to guarantee the accuracy of a BEM solution, for typical pile–soil relative stiffness values as a function of the pile diameter, the location of the interface of a two-layered soil and of the stiffness contrast. KIN SP results have been compared with simplified solutions in literature and with those obtained using a quasi-three-dimensional (3D finite element code.

  7. Protecting coastal abstraction boreholes from seawater intrusion using self-potential data

    Science.gov (United States)

    Graham, Malcolm; Butler, Adrian; MacAllister, Donald John; Vinogradov, Jan; Ijioma, Amadi; Jackson, Matthew

    2016-04-01

    We investigate whether the presence and transport of seawater can influence self-potentials (SPs) measured within coastal groundwater boreholes, with a view to using SP monitoring as part of an early warning system for saline intrusion. SP data were collected over a period of 18 months from a coastal groundwater borehole in the fractured Chalk of England. Spectral analysis of the results shows semi-diurnal fluctuations that are several orders of magnitude higher than those observed from monitoring of the Chalk more than 60 km inland, indicating a strong influence from oceanic tides. Hydrodynamic and geoelectric modelling of the coastal aquifer suggests that observed pressure changes (giving rise to the streaming potential) are not sufficient to explain the magnitude of the observed SP fluctuations. Simulation of the exclusion-diffusion potential, produced by changes in concentration across the saline front, is required to match the SP data from the borehole, despite the front being located some distance away. In late summer of 2013 and 2014, seawater intrusion occurred in the coastal monitoring borehole. When referenced to the shallowest borehole electrode, there was a characteristic increase in SP within the array, several days before any measurable increase in salinity. The size of this precursor increased steadily with depth, typically reaching values close to 0.3 mV in the deepest electrode. Numerical modelling suggests that the exclusion-diffusion potential can explain the magnitude of the precursor, but that the polarity of the change in SP cannot be replicated assuming a homogeneous aquifer. Small-scale models of idealised Chalk blocks were used to simulate the effects of discrete fractures on the distribution of SP. Initial results suggest that comparatively large reductions in voltage can develop in the matrix ahead of the front, in conjunction with a reduced or absent precursor in the vicinity of a fracture. Geophysical logging indicates the presence of a

  8. Predicting Seawater Intrusion in Coastal Groundwater Boreholes Using Self-Potential Data

    Science.gov (United States)

    Graham, M.; MacAllister, D. J.; Jackson, M.; Vinogradov, J.; Butler, A. P.

    2017-12-01

    Many coastal groundwater abstraction wells are under threat from seawater intrusion: this is exacerbated in summer by low water tables and increased abstraction. Existing hydrochemistry or geophysical techniques often fail to predict the timing of intrusion events. We investigate whether the presence and transport of seawater can influence self-potentials (SPs) measured within groundwater boreholes, with the aim of using SP monitoring to provide early warning of saline intrusion. SP data collection: SP data were collected from a coastal groundwater borehole and an inland borehole (> 60 km from the coast) in the Seaford Chalk of southern England. The SP gradient in the inland borehole was approximately 0.05 mV/m, while that in the coastal borehole varied from 0.16-0.26 mV/m throughout the monitoring period. Spectral analysis showed that semi-diurnal fluctuations in the SP gradient were several orders of magnitude higher at the coast than inland, indicating a strong influence from oceanic tides. A characteristic decrease in the gradient, or precursor, was observed in the coastal borehole several days prior to seawater intrusion. Modelling results: Hydrodynamic transport and geoelectric modelling suggest that observed pressure changes (associated with the streaming potential) are insufficient to explain either the magnitude of the coastal SP gradient or the semi-diurnal SP fluctuations. By contrast, a model of the exclusion-diffusion potential closely matches these observations and produces a precursor similar to that observed in the field. Sensitivity analysis suggests that both a sharp saline front and spatial variations in the exclusion efficiency arising from aquifer heterogeneities are necessary to explain the SP gradient observed in the coastal borehole. The presence of the precursor in the model depends also on the presence and depth of fractures near the base of the borehole. Conclusions: Our results indicate that SP monitoring, combined with hydrodynamic

  9. A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp. at Room Temperature

    Directory of Open Access Journals (Sweden)

    Fangfang Yang

    2014-03-01

    Full Text Available A novel method using ethanol was proposed for extracting lipids from wet microalga Picochlorum sp. at room temperature and pressure. In this study, Central Composite design (CCD was applied to investigate the optimum conditions of lipid extraction. The results revealed that the solvent to biomass ratio had the largest effect on lipid extraction efficiency, followed by extraction time and temperature. A high lipid extraction yield (33.04% of the dry weight was obtained under the following extraction conditions: 5 mL solvents per gram of wet biomass for 37 min with gentle stirring at room temperature. The extraction yield was comparable to that obtained by the widely used Bligh-Dyer method. Furthermore, no significant differences in the distribution of lipid classes and fatty acid composition were observed according to different extraction methods. In conclusion, these results indicated that the proposed procedure using ethanol could extract lipids from wet biomass efficiently and had giant potential for lipid extraction at large scale.

  10. Evaluation of dna extraction methods of the Salmonella sp. bacterium in artificially infected chickens eggs

    Directory of Open Access Journals (Sweden)

    Ana Cristina dos Reis Ferreira

    2015-06-01

    Full Text Available ABSTRACT. Ferreira A.C.dosR. & dos Santos B.M. [Evaluation of dna extraction methods of the Salmonella sp. bacterium in artificially infected chickens eggs.] Avaliação de três métodos de extração de DNA de Salmonella sp. em ovos de galinhas contaminados artificialmente. Revista Brasileira de Medicina Veterinária, 37(2:115-119, 2015. Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário, Av. Peter Henry Rolfs, s/n, Viçosa, MG 36571-000, Brasil. E-mail: bmsantos@ufv.br The present study evaluated the efficiency of different protocols for the genomic DNA extraction of Salmonella bacteria in chicken eggs free of specific pathogens – SPF. Seventy-five eggs were used and divided into five groups with fifteen eggs each. Three of the five groups of eggs were inoculated with enteric Salmonella cultures. One of the five groups was inoculated with Escherichia coli bacterium culture. And another group of eggs was the negative control that received saline solution 0.85% infertile. The eggs were incubated on a temperature that varied from 20 to 25°C during 24, 48 and 72 hours. Five yolks of each group were collected every 24 hours. These yolks were homogenized and centrifuged during 10 minutes. The supernatant was rejected. After the discard, PBS ph 7.2 was added and centrifuged again. The sediment obtained of each group was used for the extraction of bacterial genomic DNA. Silica particles and a commercial kit were utilized as the extraction methods. The extracted DNA was kept on a temperature of 20°C until the evaluation through PCR. The primers utilized were related with the invA gene and they were the following: 5’ GTA AAA TTA TCG CCA CGT TCG GGC AA 3’ and 5’ TCA TCG CAC CGT CAA AGG AAC C 3’. The amplification products were visualized in transilluminator with ultraviolet light. The obtained results through the bacterial DNA extractions demonstrated that the extraction method utilizing silica particles was

  11. Detection of thermophilic Campylobacter sp. in raw chicken sausages by methods ISO 10272: 2006 in Curitiba - Parana State - Brazil

    Directory of Open Access Journals (Sweden)

    K. Konell

    2014-12-01

    Full Text Available The aim of this study was the detection of Campylobacter sp. in raw chicken sausages using the methods ISO 10272-1 and ISO 10272-2. The overall prevalence of Campylobacter sp. in the samples tested was 16.67%, representing a serious risk to the health of consumers, particularly if measures guaranteeing proper cooking of foods and prevention of cross-contamination are not adopted. Furthermore, the majority of campylobacteriosis cases in humans are caused by consumption or improper handling of contaminated raw or undercooked poultry meat, which constitute the main vehicle of this infection.

  12. A Novel Method for Culturing of Leptothrix sp. Strain OUMS1 in Natural Conditions

    Directory of Open Access Journals (Sweden)

    Tomoko Suzuki

    2012-05-01

    Full Text Available Although some strains of Leptothrix spp. isolated from aquatic environments have been characterized by culturing them in laboratory conditions, they often show morphological and chemical features distinct from those found in natural environments. To resolve this discrepancy, a novel cultivation method was devised for culturing such strains in natural groundwater. Leptothrix sp. strain OUMS1 was pre-cultured in a medium lacking Fe for 2 days, and then injected into a small dialysis tube bag and immersed in a container with continuously flowing groundwater for 1–3 and 14 days. Microscopic analysis of the initial phase of sheath formation and arbitrary comparisons with medium cultures revealed that in groundwater the surface coat of the sheath comprised much thinner fibrils, and an inner sheath wall that was much thinner and more indistinct compared with medium cultures. These differences were probably attributable to poorer secretion from the cell surface in groundwater conditions. A nutrient-rich medium likely activates cell metabolism and promotes secretion, resulting in a thicker inner sheath wall and thicker outer coat fibrils. Aqueous-phase Fe was deposited on immature sheaths in a similar manner in both cultures. These results indicate that laboratory culture of isolated microbes does not always reflect their characteristics in natural environments.

  13. Inversion of self-potential anomalies caused by simple-geometry bodies using global optimization algorithms

    International Nuclear Information System (INIS)

    Göktürkler, G; Balkaya, Ç

    2012-01-01

    Three naturally inspired meta-heuristic algorithms—the genetic algorithm (GA), simulated annealing (SA) and particle swarm optimization (PSO)—were used to invert some of the self-potential (SP) anomalies originated by some polarized bodies with simple geometries. Both synthetic and field data sets were considered. The tests with the synthetic data comprised of the solutions with both noise-free and noisy data; in the tests with the field data some SP anomalies observed over a copper belt (India), graphite deposits (Germany) and metallic sulfide (Turkey) were inverted. The model parameters included the electric dipole moment, polarization angle, depth, shape factor and origin of the anomaly. The estimated parameters were compared with those from previous studies using various optimization algorithms, mainly least-squares approaches, on the same data sets. During the test studies the solutions by GA, PSO and SA were characterized as being consistent with each other; a good starting model was not a requirement to reach the global minimum. It can be concluded that the global optimization algorithms considered in this study were able to yield compatible solutions with those from widely used local optimization algorithms. (paper)

  14. Self-potential anomalies preceding tectonic and volcanic crises

    International Nuclear Information System (INIS)

    Patella, D.

    1993-01-01

    In this paper I consider a possible physical mechanism capable of explaining self-potential anomalies, which are currently observed on the ground surface prior to tectonic and volcanic activities. A rock cracking-fluid diffusion-charge polarization model is described. The electrical charge polarization is assumed to be the electrokinetic effect due to invasion of fluid into new fissures, which open inside a stressed rock material because of dilatancy, in the case of tectonic activity, and of the rising of a magma intrusion in the case of volcanic activity. (author). 10 refs, 2 figs

  15. Detecting and modeling persistent self-potential anomalies from underground nuclear explosions at the Nevada Test Site

    International Nuclear Information System (INIS)

    McKague, H.L.; Kansa, E.; Kasameyer, P.W.

    1992-01-01

    Self-potential anomalies are naturally occurring, nearly stationary electric fields that are detected by measuring the potential difference between two points on (or in) the ground. SP anomalies arise from a number of causes: principally electrochemical reactions, and heat and fluid flows. SP is routinely used to locate mineral deposits, geothermal systems, and zones of seepage. This paper is a progress report on our work toward detecting explosion-related SP signals at the Nevada Test Site (NTS) and in understanding the physics of these anomalies that persist and continue changing over periods of time that range from months to years. As background, we also include a brief description of how SP signals arise, and we mention their use in other areas such as exploring for geothermal resources and locating seepage through dams. Between the years 1988 and 1991, we surveyed the areas around seven underground nuclear tests for persistent SP anomalies. We not only detected anomalies, but we also found that various phenomena could be contributing to them and that we did not know which of these were actually occurring. We analyzed our new data with existing steady state codes and with a newly developed time-dependent thermal modeling code. Our results with the new code showed that the conductive decay of the thermal pulse from an underground nuclear test could produce many of the observed signals, and that others are probably caused by movement of fluid induced by the explosion. 25 refs

  16. Comparison of Pretreatment Methods on Vetiver Leaves for Efficient Processes of Simultaneous Saccharification and Fermentation by Neurospora sp.

    Science.gov (United States)

    Restiawaty, E.; Dewi, A.

    2017-07-01

    Lignocellulosic biomass is a potential raw material for bioethanol production. Neurospora sp. can be used to convert lignocellulosic biomass into bioethanol because of its ability to perform simultaneous saccharification and fermentation. However, lignin content, degree of polymerization, and crystallinity of cellulose contained in lignocellulosic biomass can inhibit cellulosic-biomass digestion by Neurospora sp, so that a suitable pretreatment method of lignocellulosic biomass is needed. The focus of this research was to investigate the suitable pretreatment method for vetiver leaves (Vetiveria zizanioides L. Nash) used as a raw material producing bioethanol in the process of simultaneous saccharification and fermentation (SSF) by Neurospora sp.. Vetiver plants obtained from Garut are deliberately cultivated to produce essential oils extracted from the roots of this plant. Since the vetiver leaves do not contain oil, some of harvested leaves are usually used for crafts and cattle feed, and the rest are burned. This study intended to look at other potential of vetiver leaves as a source of renewable energy. Pretreatments of the vetiver leaves were conducted using hot water, dilute acid, alkaline & dilute acid, and alkaline peroxide, in which each method was accompanied by thermal treatment. The results showed that the alkaline peroxide treatment is a suitable for vetiver leaves as indicated by the increase of cellulose content up to 65.1%, while the contents of hot water soluble, hemicellulose, lignin, and ash are 8.7%, 18.3%, 6.8%, and 1.1%, respectively. Using this pretreatment method, the vetiver leaves can be converted into bioethanol by SSF process using Neurospora sp. with a concentration of bioethanol of 6.7 g/L operated at room temperature.

  17. Evaluation of methods to detect the cotton pathogen Fusarium oxysporum f. sp. vasinfectum race 4

    Science.gov (United States)

    Fusarium wilt caused by Fusarium oxysporum f. sp. vasinfectum (Fov) is an important disease of cotton. Fov race 4, identified in the San Joaquin Valley of California, has caused serious losses and is a potential threat to US cotton production. Tests have been developed to rapidly identify race 4 i...

  18. Self-potential variations preceding earthquakes in central california

    International Nuclear Information System (INIS)

    Corwin, R.F.; Morrison, H.G.

    1977-01-01

    Two earthquakes in central California were preceded by anomalous variations in the horizontal electric field (self-potential) of the earth. The first variation was an anomaly of 90 mV amplitude across electrode dipoles of 630 and 640 m, which began 55 days before an earthquake of M=5, located 37 km NW of the dipoles. The second variation had an amplitude of 4 mV across a 300 m dipole, and began 110 hours before an event of M=2.4 located on the San Andreas fault, 2.5 km from the dipole. Streaming potentials generated by the flow of groundwater into a dilatant zone are proposed as a possible mechanism for the observed variations

  19. COMPARATIVE EVALUATION OF CONVENTIONAL VERSUS RAPID METHODS FOR AMPLIFIABLE GENOMIC DNA ISOLATION OF CULTURED Azospirillum sp. JG3

    Directory of Open Access Journals (Sweden)

    Stalis Norma Ethica

    2013-12-01

    Full Text Available As an initial attempt to reveal genetic information of Azospirillum sp. JG3 strain, which is still absence despite of the strains' ability in producing valued enzymes, two groups of conventional methods: lysis-enzyme and column-kit; and two rapid methods: thermal disruption and intact colony were evaluated. The aim is to determine the most practical method for obtaining high-grade PCR product using degenerate primers as part of routine-basis protocols for studying the molecular genetics of the Azospirillal bacteria. The evaluation includes the assessment of electrophoresis gel visualization, pellet appearance, preparation time, and PCR result of extracted genomic DNA from each method. Our results confirmed that the conventional methods were more superior to the rapid methods in generating genomic DNA isolates visible on electrophoresis gel. However, modification made in the previously developed DNA isolation protocol giving the simplest and most rapid method of all methods used in this study for extracting PCR-amplifiable DNA of Azospirillum sp. JG3. Intact bacterial cells (intact colony loaded on electrophoresis gel could present genomic DNA band, but could not be completely amplified by PCR without thermal treatment. It can also be inferred from our result that the 3 to 5-min heating in dH2O step is critical for the pre-treatment of colony PCR of Azospirillal cells.

  20. Redox potential distribution of an organic-rich contaminated site obtained by the inversion of self-potential data

    Science.gov (United States)

    Abbas, M.; Jardani, A.; Soueid Ahmed, A.; Revil, A.; Brigaud, L.; Bégassat, Ph.; Dupont, J. P.

    2017-11-01

    Mapping the redox potential of shallow aquifers impacted by hydrocarbon contaminant plumes is important for the characterization and remediation of such contaminated sites. The redox potential of groundwater is indicative of the biodegradation of hydrocarbons and is important in delineating the shapes of contaminant plumes. The self-potential method was used to reconstruct the redox potential of groundwater associated with an organic-rich contaminant plume in northern France. The self-potential technique is a passive technique consisting in recording the electrical potential distribution at the surface of the Earth. A self-potential map is essentially the sum of two contributions, one associated with groundwater flow referred to as the electrokinetic component, and one associated with redox potential anomalies referred to as the electroredox component (thermoelectric and diffusion potentials are generally negligible). A groundwater flow model was first used to remove the electrokinetic component from the observed self-potential data. Then, a residual self-potential map was obtained. The source current density generating the residual self-potential signals is assumed to be associated with the position of the water table, an interface characterized by a change in both the electrical conductivity and the redox potential. The source current density was obtained through an inverse problem by minimizing a cost function including a data misfit contribution and a regularizer. This inversion algorithm allows the determination of the vertical and horizontal components of the source current density taking into account the electrical conductivity distribution of the saturated and non-saturated zones obtained independently by electrical resistivity tomography. The redox potential distribution was finally determined from the inverted residual source current density. A redox map was successfully built and the estimated redox potential values correlated well with in

  1. Specific storage and hydraulic conductivity tomography through the joint inversion of hydraulic heads and self-potential data

    Science.gov (United States)

    Ahmed, A. Soueid; Jardani, A.; Revil, A.; Dupont, J. P.

    2016-03-01

    Transient hydraulic tomography is used to image the heterogeneous hydraulic conductivity and specific storage fields of shallow aquifers using time series of hydraulic head data. Such ill-posed and non-unique inverse problem can be regularized using some spatial geostatistical characteristic of the two fields. In addition to hydraulic heads changes, the flow of water, during pumping tests, generates an electrical field of electrokinetic nature. These electrical field fluctuations can be passively recorded at the ground surface using a network of non-polarizing electrodes connected to a high impedance (> 10 MOhm) and sensitive (0.1 mV) voltmeter, a method known in geophysics as the self-potential method. We perform a joint inversion of the self-potential and hydraulic head data to image the hydraulic conductivity and specific storage fields. We work on a 3D synthetic confined aquifer and we use the adjoint state method to compute the sensitivities of the hydraulic parameters to the hydraulic head and self-potential data in both steady-state and transient conditions. The inverse problem is solved using the geostatistical quasi-linear algorithm framework of Kitanidis. When the number of piezometers is small, the record of the transient self-potential signals provides useful information to characterize the hydraulic conductivity and specific storage fields. These results show that the self-potential method reveals the heterogeneities of some areas of the aquifer, which could not been captured by the tomography based on the hydraulic heads alone. In our analysis, the improvement on the hydraulic conductivity and specific storage estimations were based on perfect knowledge of electrical resistivity field. This implies that electrical resistivity will need to be jointly inverted with the hydraulic parameters in future studies and the impact of its uncertainty assessed with respect to the final tomograms of the hydraulic parameters.

  2. Bacterial diversity associated with the rotifer Brachionus plicatilis sp. complex determined by culture-dependent and -independent methods.

    Science.gov (United States)

    Ishino, Ryota; Iehata, Shunpei; Nakano, Miyo; Tanaka, Reiji; Yoshimatsu, Takao; Maeda, Hiroto

    2012-03-01

    The bacterial communities associated with rotifers (Brachionus plicatilis sp. complex) and their culture water were determined using culture-dependent and -independent methods (16S rRNA gene clone library). The bacterial communities determined by the culture-independent method were more diverse than those determined by the culture-dependent method. Although the culture-dependent method indicated the bacterial community of rotifers was relatively similar to that of the culture water, 16S rRNA gene clone library analyses revealed a great difference between the two microbiotas. Our results suggest that most bacteria associated with rotifers are not easily cultured using conventional methods, and that the microbiota of rotifers do not correspond with that of the culture water completely.

  3. Self-potential signals caused by eruptions of the Galeras volcano – Colombia

    Directory of Open Access Journals (Sweden)

    Greinwald S.

    2007-12-01

    Full Text Available The National Institute of Geology and Mining, INGEOMINAS - Colombia, and the Federal Institute for Geosciences and Natural Resources, BGR - Germany, perform since 1997 as a joint venture
    multi-parameter measurements at the Galeras volcano, in the southwest of Colombia. Since the end of 1998 the Multi-parameter Station at Galeras (Estación Multiparámetro del Galeras - EMG includes the continuous monitoring of electromagnetic variations. The electromagnetic (EM station is located at the north-eastern foot walls of the central cone inside the caldera.
    During almost six years of electromagnetic monitoring, the data did not show significant variations of the electromagnetic field, which could be related to the volcanic activity. In July 2004 a new active period of Galeras began with two strong ash emissions. During both emissions strong self potential- (SP signals were recorded lasting for several hours. The present paper will present the data which could give some indications on the movements of liquids during ash emissions.

  4. Response surface method optimization of ectoine fermentation medium with moderate halophilic bacteria Halomonas sp. H02

    Science.gov (United States)

    Li, T. T.; Qu, A.; Yuan, X. N.; Tan, F. X.; Li, X. W.; Wang, T.; Zhang, L. H.

    2017-07-01

    Moderate halophilic bacteria are of halophilic bacteria whose suitable growth of NaCl is 5-10%. When the moderate halophilic bacteria response to high osmotic stress, the intracellular will synthesize small organic molecule compatible solutes. Ectoine, which is the major synthetic osmotic compatible solutes for moderate halophilic bacteria, can help microbial enzymes, nucleic acids and the whole cell resist to hypertonic, high temperature, freezing and other inverse environment. In order to increase the Ectoine production of Moderate halophilic bacteria Halomonas sp. H02, the Ectoine fermentation medium component was optimized by Plackett-Burman (PB) and Response Surface Methodology (RSM) based on the principle of non-complete equilibrium The results of PB experiments showed that the three main influencing factors of Moderate halophilic bacteria Halomonas sp. H02 synthesis Ectoine culture medium were C5H8NNaO4 concentration, NaCl concentration and initial pH. According to the center point of the steepest climbing experiment, the central combination design experiment was used to show that the model is consistent with the actual situation. The optimum combination of three influencing factors were C5H8NNaO4 41 g/L, NaCl 87.2 g/L and initial pH 5.9, and the predicted amount of Ectoine was 1835.8 mg/L, increased by 41.6%.

  5. The effect of thallus spreading method on productivity of Gracilaria sp. culture

    Science.gov (United States)

    Hidayatulbaroroh, R.; Nurhudah, M.; Edy, M. H.; Suharyadi

    2018-04-01

    The aim of this study was to determine growth of (Gracilaria sp.) with different spreading time of thallus. The study was conducted from March to April 2017 in pond located in Domas Village, Serang Region, Banten Province. The experiment followed completely randomized design with the treatment of different time on spreading of seaweed thallus during the culture period (45 days). Treatments were without spreading (as control), spreading every 2 weeks, and spreading every 3 weeks. The observed variables were weight of seaweed thallus and several water quality parameters. Analysis of seaweed weight used ANOVA test and Tukey HSD test. The results showed that the spread seaweed thallus had a significant effect on weight gain in 0.05 level. It used 100 gram Gracilaria sp. as initial weight, treatment without spreading thallus produced 508 gram, spreading every 2 weeks produced 906 gram and spreading every 3 weeks produced 790 gram. Based on the weight gain of thallus, seaweed culture by spreading thallus every 3 weeks and 2 weeks seem to be able to increase productivity by 56 % and 78 %, respectively.

  6. Molecular profiles of Venezuelan isolates of Trypanosoma sp. by random amplified polymorphic DNA method.

    Science.gov (United States)

    Perrone, T M; Gonzatti, M I; Villamizar, G; Escalante, A; Aso, P M

    2009-05-12

    Nine Trypanosoma sp. Venezuelan isolates, initially presumed to be T. evansi, were collected from three different hosts, capybara (Apure state), horse (Apure state) and donkey (Guarico state) and compared by the random amplification polymorphic DNA technique (RAPD). Thirty-one to 46 reproducible fragments were obtained with 12 of the 40 primers that were used. Most of the primers detected molecular profiles with few polymorphisms between the seven horse, capybara and donkey isolates. Quantitative analyses of the RAPD profiles of these isolates revealed a high degree of genetic conservation with similarity coefficients between 85.7% and 98.5%. Ten of the primers generated polymorphic RAPD profiles with two of the three Trypanosoma sp. horse isolates, namely TeAp-N/D1 and TeGu-N/D1. The similarity coefficient between these two isolates and the rest, ranged from 57.9% to 68.4% and the corresponding dendrogram clustered TeAp-N/D1 and Te Gu-N/D1 in a genetically distinct group.

  7. Development of a Simple and Effective Bioassay Method to Evaluate Resistance of Watermelon Plants to Fusarium oxysporum f. sp. niveum

    Directory of Open Access Journals (Sweden)

    Eun Ju Jo

    2017-06-01

    Full Text Available Root-dipping inoculation method has been widely used to determine the resistance of watermelon to Fusarium oxysporum f. sp. niveum causing Fusarium wilt. Although this method leads to the precise results of plant disease responses, more rapid and efficient assay methods have been still required because the root-dipping inoculation method is labor-intensive and time-consuming. In this study, we established a simple and effective bioassay method based on the comparison of various inoculation methods and growth conditions. To develop the system, the occurrence of Fusarium wilt on four resistant and susceptible cultivars was investigated by four different inoculation methods, root-dipping, scalpel, tip and soil-drenching methods. Of these inoculation methods, scalpel method resulted in clear plant disease resistance responses with the simplicity. With the use of scalpel method, we also explored the disease development of the cultivars depending on inoculum concentration, growth stage of seedlings, and incubation temperature after inoculation. Furthermore, we found that the resistance degrees of 23 cultivars derived by scalpel inoculation method were similar to the results by root-dipping method established previously.

  8. Shape and depth determinations from second moving average residual self-potential anomalies

    International Nuclear Information System (INIS)

    Abdelrahman, E M; El-Araby, T M; Essa, K S

    2009-01-01

    We have developed a semi-automatic method to determine the depth and shape (shape factor) of a buried structure from second moving average residual self-potential anomalies obtained from observed data using filters of successive window lengths. The method involves using a relationship between the depth and the shape to source and a combination of windowed observations. The relationship represents a parametric family of curves (window curves). For a fixed window length, the depth is determined for each shape factor. The computed depths are plotted against the shape factors, representing a continuous monotonically increasing curve. The solution for the shape and depth is read at the common intersection of the window curves. The validity of the method is tested on a synthetic example with and without random errors and on two field examples from Turkey and Germany. In all cases examined, the depth and the shape solutions obtained are in very good agreement with the true ones

  9. First measurement of the 34S(p ,γ )35Cl reaction rate through indirect methods for presolar nova grains

    Science.gov (United States)

    Gillespie, S. A.; Parikh, A.; Barton, C. J.; Faestermann, T.; José, J.; Hertenberger, R.; Wirth, H.-F.; de Séréville, N.; Riley, J. E.; Williams, M.

    2017-08-01

    Sulphur isotopic ratio measurements may help to establish the astrophysical sites in which certain presolar grains were formed. Nova model predictions of the 34S/32S ratio are, however, unreliable due to the lack of an experimental 34S(p ,γ )35Cl reaction rate. To this end, we have measured the 34S(3He,d )35Cl reaction at 20 MeV using a high resolution quadrupole-dipole-dipole-dipole magnetic spectrograph. Twenty-two levels over 6.2 MeV rate has been determined using a Monte Carlo method. Hydrodynamic nova model calculations have been performed using this new reaction rate. These models show that remaining uncertainties in the 34S(p ,γ ) rate affect nucleosynthesis predictions by less than a factor of 1.4, and predict a 34S/32S isotopic ratio of 0.014-0.017. Since recent type II supernova models predict 34S/32S=0.026 -0.053 , the 34S/32S isotopic ratio may be used, in conjunction with other isotopic signatures, to distinguish presolar grains from oxygen-neon nova and type II supernova origin. Our results address a key nuclear physics uncertainty on which recent considerations discounting the nova origin of several grains depend.

  10. New methods reveal oldest known fossil epiphyllous moss: Bryiidites utahensis gen. et sp. nov. (Bryidae).

    Science.gov (United States)

    Barclay, Richard S; McElwain, Jennifer C; Duckett, Jeffrey G; van Es, Maarten H; Mostaert, Anika S; Pressel, Silvia; Sageman, Bradley B

    2013-12-01

    Epiphyllous bryophytes are a highly characteristic feature of many humid tropical forest ecosystems. In contrast to the extensive fossil record for the leaves of their host plants, the record is virtually nonexistent for the epiphylls themselves, despite a fossil record for mosses that begins in the Middle Carboniferous Period, 330 million years ago. Epifluorescence optical microscopy, scanning electron microscopy, and atomic force microscopy were employed to investigate an intimate association between a newly discovered epiphyllous moss and a Lauraceae plant host from the middle Cretaceous. We describe the oldest fossil specimen of an epiphyllous moss, Bryiidites utahensis gen. et sp. nov., identified from an individual specimen only 450 µm long, situated on an approximately one millimeter square fossil leaf fragment. The moss epiphyll is exquisitely preserved as germinating spores and short-celled protonemata with transverse and oblique cross-walls closely matching those of extant epiphyllous mosses on the surface of the plant-leaf hosts. The extension of the epiphyll record back to the middle Cretaceous provides fossil evidence for the appearance of epiphyllous mosses during the diversification of flowering plants, at least 95 million years ago. It also provides substantive evidence for a tropical maritime climate in central North America during the middle Cretaceous.

  11. Evaluation of mathematical methods for predicting optimum dose of gamma radiation in sugarcane (Saccharum sp.)

    International Nuclear Information System (INIS)

    Wu, K.K.; Siddiqui, S.H.; Heinz, D.J.; Ladd, S.L.

    1978-01-01

    Two mathematical methods - the reversed logarithmic method and the regression method - were used to compare the predicted and the observed optimum gamma radiation dose (OD 50 ) in vegetative propagules of sugarcane. The reversed logarithmic method, usually used in sexually propagated crops, showed the largest difference between the predicted and observed optimum dose. The regression method resulted in a better prediction of the observed values and is suggested as a better method for the prediction of optimum dose for vegetatively propagated crops. (author)

  12. ExaSP2

    Energy Technology Data Exchange (ETDEWEB)

    2017-09-08

    ExaSP2 is a reference implementation of typical linear algebra algorithms and workloads for a quantum molecular dynamics (QMD) electronic structure code. The algorithm is based on a recursive second-order Fermi-Operator expansion method (SP2) and is tailored for density functional based tight-binding calculations of material systems.

  13. Characterization of sp3 bond content of carbon films deposited by high power gas injection magnetron sputtering method by UV and VIS Raman spectroscopy

    Science.gov (United States)

    Zdunek, Krzysztof; Chodun, Rafał; Wicher, Bartosz; Nowakowska-Langier, Katarzyna; Okrasa, Sebastian

    2018-04-01

    This paper presents the results of investigations of carbon films deposited by a modified version of the magnetron sputtering method - HiPGIMS (High Power Gas Injection Magnetron Sputtering). In this experiment, the magnetron system with inversely polarized electrodes (sputtered cathode at ground potential and positively biased, spatially separated anode) was used. This arrangement allowed us to conduct the experiment using voltages ranging from 1 to 2 kV and a power supply system equipped with 25/50 μF capacitor battery. Carbon films were investigated by VIS/UV Raman spectroscopy. Sp3/sp2 bonding ratio was evaluated basing the elementary components of registered spectra. Our investigation showed that sp3 bond content increases with discharge power but up to specific value only. In extreme conditions of generating plasma impulses, we detected a reversed relation of the sp3/sp2 ratio. In our opinion, a energy of plasma pulse favors nucleation of a sp3 phase because of a relatively higher ionization state but in extreme cases the influence of energy is reversed.

  14. Characterization of sp3 bond content of carbon films deposited by high power gas injection magnetron sputtering method by UV and VIS Raman spectroscopy.

    Science.gov (United States)

    Zdunek, Krzysztof; Chodun, Rafał; Wicher, Bartosz; Nowakowska-Langier, Katarzyna; Okrasa, Sebastian

    2018-04-05

    This paper presents the results of investigations of carbon films deposited by a modified version of the magnetron sputtering method - HiPGIMS (High Power Gas Injection Magnetron Sputtering). In this experiment, the magnetron system with inversely polarized electrodes (sputtered cathode at ground potential and positively biased, spatially separated anode) was used. This arrangement allowed us to conduct the experiment using voltages ranging from 1 to 2kV and a power supply system equipped with 25/50μF capacitor battery. Carbon films were investigated by VIS/UV Raman spectroscopy. Sp 3 /sp 2 bonding ratio was evaluated basing the elementary components of registered spectra. Our investigation showed that sp 3 bond content increases with discharge power but up to specific value only. In extreme conditions of generating plasma impulses, we detected a reversed relation of the sp 3 /sp 2 ratio. In our opinion, a energy of plasma pulse favors nucleation of a sp 3 phase because of a relatively higher ionization state but in extreme cases the influence of energy is reversed. Copyright © 2018 Elsevier B.V. All rights reserved.

  15. A Sensitive and Specific PCR Based Method for Identification of Cryptosporidium Sp. Using New Primers from 18S Ribosomal RNA

    Directory of Open Access Journals (Sweden)

    M Heydarnezhadi

    2011-09-01

    Full Text Available Background: The main goal of the present study was to develop a new sensitive and specific PCR based method for Identification of Cryptosporidium sp. using novel primers from 18S ribosomal RNA. Cryptosporidi­osis in high-risk host groups particularly in neonates and immuno-compromised individuals may result in death. To the best of our knowledge this is the first study regarding develop a new PCR based method to diagnose the cryptosporidiosis in Iran.Methods: A total of 850 human fecal samples from patients clinically suspected to cryptosporidiosis and 100 healthy and diarrheic cattle stool specimens were collected. The simplified formol-ether concentration method was carried out for all samples. They were then examined microscopically by modified Ziehl-Neel­sen staining method. Total DNA was extracted by QIA amp DNA stool mini kit was carried out by using designed prim­ers.Results: Twenty nine cases of cryptosporidiosis infection in human and 30 samples from cattle microscopi­cally were posi­tive. The described primary and nested PCR method could detect all Cryptospori­dium positive samples from human and cattle. Regards to suspected negative samples in pri­mary PCR examination, the Nested PCR could ap­prove two more positive results. Furthermore, Nested PCR analysis was able to detect one more case which was nega­tive in both microscopically examination and primary PCR. Specificity of the test was 100%. Sensitivity of Nested PCR in comparison to our gold standard; microscopy after Ridley concentration modified ziehl-Neelsen, was 100 %.Conclusion: Our developed PCR based method by using new primers devised from 18S ribosomal RNA revealed the ability for identification of the Cryptosporidium species such as C. parvum and C. huminis with high specificity and sensitivity.

  16. Comparative Analysis of the Genomic DNA Isolation Methods on Inula sp. (Asteraceae

    Directory of Open Access Journals (Sweden)

    Emre SEVİNDİK

    2016-12-01

    Full Text Available Simple, fast, low-cost and high throughput protocols are required for DNA isolation of plant species. In this study, phenol chloroform isoamyl alcohol and commercial (Sigma DNA isolation kit methods were applied on some Inula species that belong to Asteraceae family. Genomic DNA amounts, A260, A280, A260/A230 and purity degrees (A260/A280 that were obtained through both methods were measured through electrophoresis and spectrophotometer. Additionally, PCR amplification was realized by primer pairs specific to nrDNA ITS, cpDNA ndhF (972F-1603R and trnL-F regions. Results showed that maximum genomic DNA in nanograms obtained by phenol chloroform isoamyl alcohol method. The study also revealed that I. macrocephala had the maximum DNA and I. heterolepis had the minimum DNA amount. A260/A280 purity degrees showed that the highest and lowest purity in gDNAs obtained through phenol-choloform isoamyl alcohol method were in I.aucheriana and I. salicina, respectively. The highest and lowest purity degrees of gDNAs obtained through commercial kit was observed in I. fragilis and I. macrocephala samples, respectively. PCR amplification results showed that while band profiles of each three regions (ITS, trnL-F and ndhF did not yield positive results in PCR amplifications using phenol-choloform isoamyl alcohol method; PCR band profiles obtained through commercial kit yielded positive results. As a result, it is fair to say that the relation of genomic DNA with PCR was found to be more efficient although the maximum amount of genomic DNA was obtained through phenol chloroform isoamyl alcohol method.

  17. High-performance whole core Pin-by-Pin calculation based on EFEN-SP_3 method

    International Nuclear Information System (INIS)

    Yang Wen; Zheng Youqi; Wu Hongchun; Cao Liangzhi; Li Yunzhao

    2014-01-01

    The EFEN code for high-performance PWR whole core pin-by-pin calculation based on the EFEN-SP_3 method can be achieved by employing spatial parallelization based on MPI. To take advantage of the advanced computing and storage power, the entire problem spatial domain can be appropriately decomposed into sub-domains and the assigned to parallel CPUs to balance the computing load and minimize communication cost. Meanwhile, Red-Black Gauss-Seidel nodal sweeping scheme is employed to avoid the within-group iteration deterioration due to spatial parallelization. Numerical results based on whole core pin-by-pin problems designed according to commercial PWRs demonstrate the following conclusions: The EFEN code can provide results with acceptable accuracy; Communication period impacts neither the accuracy nor the parallel efficiency; Domain decomposition methods with smaller surface to volume ratio leads to greater parallel efficiency; A PWR whole core pin-by-pin calculation with a spatial mesh 289 × 289 × 218 and 4 energy groups could be completed about 900 s by using 125 CPUs, and its parallel efficiency is maintained at about 90%. (authors)

  18. Preservation of Steamed Fish (Rastrelliger Sp With Combine Method Using Sodium Acetate, Lactic Acid Bacteria Culture and Vacuum Packaging

    Directory of Open Access Journals (Sweden)

    Betty Sri Laksmi Jenie, . Nuratifa, . Suliantari

    2001-04-01

    Full Text Available This study was carried out to improve the safety and shelf life of cooked kembung fish (Rastrelliger sp, a traditional food called pindang fish. Fresh eviscerated fish was fisrt soaked in 2% NaCl solution for 15 minutes, drained, washed with tap water and drained again. Sodium chloride at 12% concentration (w/w was distributed on the whole surface of the fish. Fish was then laid on a wooden basket inside a clay pot, steamed for 30 minutes, and then cooled. Combine method applied to the steamed fish (pindang was soaking in a mixed culture of Lactobacillus plantarum kik and Lactococcus lactis subsp. cremoris in the ratio of 2 : 1 (v/v containing 4% Na-acetate for 2 hrs and after draining, the product was vacuum packed. The result showed that the combine method using mixed culture of lactic acid bacteria containing 4% Na-acetate could reduce the growth of Staphylococcus aureus by 3-6 log units, decrease the TMA (Trimethylamine content and maintain the organoleptic properties (texture, appearance and odor of pindang fish during 6 days storage at room temperature. Control treatment without 4% Na-acetate could only keep the pindang fish for 4 days. Vacuum and nonvacuum packaging did not show any significant difference.

  19. Lead-lead isotope method. Application to migmatites and associaded rocks of the region of Sao Jose dos Campos, SP

    International Nuclear Information System (INIS)

    Babinski, M.

    1988-01-01

    Establishment of chemical and analytical procedures, preparation and purification of reagents and the mounting of a clean laboratory (class 100) for the Pb-Pb isotope dating of whole rock and mineral samples are discussed in this work. Two different methods of dissolution of rock samples and chemical separation of lead using anionic resin were tried in a conventional chemical laboratory using analytical grade reagents. The samples analysed were paleossome and neossome of migmatites from Sao Jose dos Campos, SP, and from the results obtained the analytical method to be employed was chosen. The results obtained by this method agree well with those of the Geochronology Laboratory at Oxford, for the same samples. Applying the established analytical procedure, migmatites and associated gneisses from the region of Sao Jose dos Campos were dated by Pb/Pb method. Some of the samples were also dated by Rb-Sr method. The lead-lead isochron age of 2355 +- 85 M.a. (μ 1 = 8.33) for the paleossome may be interpreted as the age of the metamorphism for these rocks, which were formed probably during the Archean. The age data for the phacoidal gneisses show a similar geological evolution, whereas the 2414 +- 800 M.A. Pb-Pb isochron age for the protomylonitic gneisses indicates a disturbed Pb-Pb isotope system. The neossome samples define a Pb-Pb isochron age of 1388 +- 290 M.A. (μ1 = 8.15), which may be interpreted as the age of migmatization. The calculated Pb-Pb ages for two alkali feldspars from the migmatites are devoid of any geological significance. The age values obtained by the Rb-Sr isotope system for the neossome and paleossome of the migmatites agree with the Pb/Pb data within experimental errors. However, the study shows that results of the Rb-Sr data alone cannot be interpreted unambiguously with out comparison with data from other isotope systems. (author) [pt

  20. Studies on Methods for Detection of Salmonella SP. in Meat with Regard to Equivalency and Compatibility

    Energy Technology Data Exchange (ETDEWEB)

    Paulsen, P.; Smulders, F. J.M. [Institute for Meat Hygiene, Meat Technology and Food Science, University for Veterinary Medicine, Vienna (Austria); Girma, Z. [Addis Ababa University, Debre Zeit (Ethiopia); Farghaly, R. [South Valley University, Qena (Egypt)

    2005-01-15

    This contribution summarizes research activities on the evaluation of methods for detection of Salmonella in meat, especially poultry. The following items were under study: (1) studies on motility media for detection of Salmonella spp.: evaluation of MSRV media of different manufacturers; evaluation of novobiocine supplementation to MSRV and DIASALM media; abuse studies of incubation temperature; comparison of DIASALM medium vs. MSRV; (2) Salmonella antigen detection by a commercial EIA (Vidas System). The findings and information from other sources (references, technical papers) are to be combined in a database to give a comprehensive overview on the currently applied methodology. Basic considerations on the structure of this database are demonstrated. (author)

  1. In vitro adhesion assay of lactic acid bacteria, Escherichia coli and Salmonella sp. by microbiological and PCR methods

    Directory of Open Access Journals (Sweden)

    Didier Montet

    2006-03-01

    Full Text Available In vitro adhesion assay using Lactobacillus reuteri KUB-AC5 as a test strain has been studied by applying simple PCR reaction together with image analysis and plate count techniques. Critical factor affecting the PCR method was quality and quantity of DNA. The cell lysis technique was modified to optimize this method. Thus, lysozyme and proteinase K were added to lyse the cells, followed by SDS solution to obtain a complete cell lysis. Only PCR products from total cells (TC were obtained, with low consistency, but none from cells bound to mucus (BC at either 0.1 or 0.5 mg/mL concentration. It was hypothesized that the attached cells might not be extracted into the cell suspension. Therefore, 1% SDS solution and 0.1M NaOH were used directly in the extraction. As expected, PCR products were observed when both TC and BC were used as a DNA template. Adhesion appeared at a wide range of 0-45%, with low consistency. Therefore, a simple microbiological method (plate count was used. The extraction of bound cells into cell suspension was critical in this method. Extraction times of 20, 60, 120 and 150 min were tried. Results showed that maximum cell number was obtained with 120 min extraction. L. reuteri KUB-AC5, L. reuteri KUB-AC16, L. reuteri KUB-AC20, L. salivarius KUB-AC21, L. acidophilus KV-1, Escherichia coli E010, Salmonella sp. S003, E. coli ATCC8739, and S. typhimurium ATCC 13311 exhibited adhesion activity of 21.6%, 0.8%, 5.7%, 1.1%, 23.1%, 10.7%, 10.3%, 4.4% and 3.2%, respectively. Among the 9 types of microorganisms tested L. acidophilus KV-1 and L. reuteri KUB-AC5 showed higher adhesion activity than the others.

  2. Antipersistent dynamics in short time scale variability of self-potential signals

    OpenAIRE

    Cuomo, V.; Lanfredi, M.; Lapenna, V.; Macchiato, M.; Ragosta, M.; Telesca, L.

    2000-01-01

    Time scale properties of self-potential signals are investigated through the analysis of the second order structure function (variogram), a powerful tool to investigate the spatial and temporal variability of observational data. In this work we analyse two sequences of self-potential values measured by means of a geophysical monitoring array located in a seismically active area of Southern Italy. The range of scales investigated goes from a few minutes to several days. It is shown that signal...

  3. Dating of aragonite and dolomite from Devil's cave - SP, by dosimetric thermoluminescent method (DTL) and ESR

    International Nuclear Information System (INIS)

    Tatumi, S.H.

    1987-01-01

    We have investigated TL (Thermoluminescence) and ESR (electron spin resonance) properties of aragonite and dolomite found at ''Caverna do Diabo (Devil's cave)'' in the state of Sao Paulo to determine the ages of these geological materials. The aragonite, which is not a thermoluminescent material, shows one ESR signal whit g = 2.003. We obtained the age of 1.1 x 10 5 years and the growth rate of 1 μ m/year by the sample calibration with additional Y irradiation. The dolomite gives a TL glow curve with three distinct peaks at 261, 334 and 395 0 C. We found that the fit for the TL glow curve to second-order kinetics can be considerered as very acceptable. Using the second TL peak we obtained the age of 9.4 x 10 5 years and the compatible values of the activation energy (1.90 ± 0,07 eV) by the following methods: Hyperbolic heating, isothermal decay and fractional glow technique. We observed many ESR absorption signals in the dolomite. Three principal signal com g = 2.002, 2.003 and 2.005 were studied. We found that the first and second ESR signals were atributed to the CO 2 - and/or CO 3 3- centers and the last to the CO 3 - centers, and observed that the first ESR signal might related with the second TL peak and the TL sensitivity were associated with the concentration of Mn 2+ [pt

  4. [Application of PBL combined with SP method in during-course practice of endodontics for undergraduate dental students].

    Science.gov (United States)

    Shao, Li-Na; Qiu, Li-Hong; Zhan, Fu-Liang; Xue, Ming

    2016-10-01

    To apply problem-based learning (PBL) combined with standardized patients(SP) in during-course practice of endodontics for undergraduate dental students, in order to improve the teaching quality. One hundred and four undergraduate dental students of China Medical University School of Stomatology were randomly divided into 2 groups, 52 students in each group. One group were taught with PBL combined with SP while the other group with lecture-based learning (LBL) alone. The teaching effect was measured with examination and questionnaire survey. The data were analyzed by Student's t test using SPSS 11.5 software package. Students in PBL combined with SP group was better than LBL group in case analysis, didactic tests, practical tests and total scores, and there was significant difference between the two groups (Pendodontics to undergraduate dental students.

  5. Description of recovery method used for curdlan produced by Agrobacterium sp. IFO 13140 and its relation to the morphology and physicochemical and technological properties of the polysaccharide.

    Science.gov (United States)

    Mangolim, Camila Sampaio; Silva, Thamara Thaiane da; Fenelon, Vanderson Carvalho; Koga, Luciana Numata; Ferreira, Sabrina Barbosa de Souza; Bruschi, Marcos Luciano; Matioli, Graciette

    2017-01-01

    Curdlan is a linear polysaccharide considered a dietary fiber and with gelation properties. This study evaluated the structure, morphology and the physicochemical and technological properties of curdlan produced by Agrobacterium sp. IFO 13140 recovered by pre-gelation and precipitation methods. Commercial curdlan submitted or otherwise to the pre-gelation process was also evaluated. The data obtained from structural analysis revealed a similarity between the curdlan produced by Agrobacterium sp. IFO 13140 (recovered by both methods) and the commercial curdlans. The results showed that the curdlans evaluated differed significantly in terms of dispersibility and gelation, and only the pre-gelled ones had significant potential for food application, because this method influence on the size of the particles and in the presence of NaCl. In terms of technological properties, the curdlan produced by Agrobacterium sp. IFO 13140 (pre-gelation method) had a greater water and oil holding capacity (64% and 98% greater, respectively) and a greater thickening capacity than the pre-gelled commercial curdlan. The pre-gelled commercial curdlan displayed a greater gelling capacity at 95°C than the others. When applied to food, only the pre-gelled curdlans improved the texture parameters of yogurts and reduced syneresis. The curdlan gels, which are rigid and stable in structure, demonstrated potential for improving the texture of food products, with potential industrial use.

  6. Advanced Self-Potential Inversion. Development and Use for Investigating Natural Recharge Processes at the ORNL IFC

    Energy Technology Data Exchange (ETDEWEB)

    Revil, Andre [Colorado School of Mines, Golden, CO (United States)

    2013-01-15

    Understanding the influence of coupled biological, chemical, and hydrological processes on subsurface contaminant behavior at multiple scales is a prerequisite for developing effective remedial approaches, whether they are active remediation or natural attenuation strategies. To develop this understanding, methods are needed that can measure critical components of the natural system in real time. The self-potential method corresponds to the passive measurement of the distribution of the electrical potential at the surface of the Earth or in boreholes. This method is very complemetary to other geophysical methods like DC resistivity and induced polarization. In this report, we summarize of research efforts to advance the theory of low-frequency geoelectrical methods and their applications to the contaminant plumes in the vicinity of the former S-3 settling basins at Oak Ridge, TN.

  7. Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages

    Directory of Open Access Journals (Sweden)

    Thalyta Marina Benetti

    2012-12-01

    Full Text Available The aim of this study was to determine the prevalence of Listeria sp. in refrigerated sausages, and to compare the performance of the selective plating media employed in the ISO 11290-1 method (PALCAM and Oxford agars with chromogenic agars (Chromogenic Listeria agars CM 1080 (OCLA and CM 1084. The prevalence of Listeria sp. detected was 52.9%, comprising 13.7% L. monocytogenes strains. The efficacy of the four agars for the isolation of L. monocytogenes proved to be satisfactory. Despite differences in composition of the chromogenic media assessed, these disparities did not affect concordance among results. However, PALCAM agar was shown to suppress other microorganisms more effectively, being more applicable for detecting Listeria strains present in lower quantities. Based on these results, the use of PALCAM agar, in combination with a chromogenic media, is recommended for enhanced isolation of atypical Listeria sp. strains in meat products.Este estudo teve como objetivo a análise da prevalência de Listeria sp. em linguiças resfriadas e a comparação dos meios seletivos utilizados no plaqueamento do método ISO 11290-1 (Ágar PALCAM e Ágar Oxford, e ágares cromogênicos (Ágares Listeria Cromogênico CM 1080 (OCLA e CM 1084 (ISO. A frequência de Listeria sp. foi de 52,9%, sendo que destas, 13,7% corresponderam à L. monocytogenes. A eficácia dos quatro ágares para o isolamento de L. monocytogenes demonstrou-se satisfatória. Apesar de haver algumas diferenças nas composições dos meios cromogênicos analisados, estas não pareceram influenciar nas concordâncias entre os resultados expressos. Contudo, o ágar PALCAM mostrou-se mais eficaz na supressão de outros micro-organismos, aumentando, assim, a possibilidade de detecção de espécies de Listeria presentes em número reduzido. Através deste trabalho sugere-se a utilização do ágar PALCAM associado a um meio cromogênico para aumentar a chance de isolamento de cepas at

  8. Antipersistent dynamics in short time scale variability of self-potential signals

    Directory of Open Access Journals (Sweden)

    M. Ragosta

    2000-06-01

    Full Text Available Time scale properties of self-potential signals are investigated through the analysis of the second order structure function (variogram, a powerful tool to investigate the spatial and temporal variability of observational data. In this work we analyse two sequences of self-potential values measured by means of a geophysical monitoring array located in a seismically active area of Southern Italy. The range of scales investigated goes from a few minutes to several days. It is shown that signal fluctuations are characterised by two time scale ranges in which self-potential variability appears to follow slightly different dynamical behaviours. Results point to the presence of fractal, non stationary features expressing a long term correlation with scaling coefficients which are the clue of stabilising mechanisms. In the scale ranges in which the series show scale invariant behaviour, self-potentials evolve like fractional Brownian motions with anticorrelated increments typical of processes regulated by negative feedback mechanisms (antipersistence. On scales below about 6 h the strength of such an antipersistence appears to be slightly greater than that observed on larger time scales where the fluctuations are less efficiently stabilised.

  9. Development of geothermal field following the 2000 eruption of Usu volcano as revealed by ground temperature, resistivity and self-potential variations

    Directory of Open Access Journals (Sweden)

    T. Mogi

    2007-06-01

    Full Text Available The 2000 eruption of Usu volcano, NE Japan, took place on the foot of the somma, and formed a cryptodome of 65 m high accompanying numerous faults. We made repeated measurements of ground temperature, Self-Potential (SP and electrical resistivity, in order to clarify the mechanism of development of the newly formed geothermal field on the fault zone. Prior to the expansion of the geothermal field, we detected a resistive zone at the center of the geothermal zone and it supposed to evidence that the zone involving dry steam phase had been formed beneath the fault zone. A rapid expansion of the geothermal field followed along the fault zone away from the craters. The place of maximum amplitude of the SP field also migrated following the expansion of the high ground temperature zone. The high resistive part has shrunk as a consequence of the progress of condensation to warm the surroundings. Based on the observations, we delineated the process of the hydrothermal circulation. Considering the topographic effect of the SP field observed on the highly permeable zone in the Usu somma, the potential flow along the slope of the soma was expected to play an important role to promote the rapid expansion of the geothermal field and the migration of the most active part.

  10. Self-potential chenges associated with volcanic activity: Short-term signals associated with March 9, 1998 eruption on La Fournaise volcano (Reunion Island

    Directory of Open Access Journals (Sweden)

    P. Yvetot

    2001-06-01

    Full Text Available After six years of quietness La Fournaise volcano entered into activity on March 9, 1998. Fissures opened gradually downwards on the northern flank of the cone. Two cones, Kapor and Krafft built, from which lava poured until September 1998. Several other vents opened during this eruption. Mappings, surveys, and continuous recordings of the Self-Potential have been performed on the volcano for twenty years. SP mappings disclose the variability of large scale SP anomalies due to the modification of the hydrothermal system over some ten years. Most of the eruptions take place along a Main Fracture Zone (MFZ in which ground water flows prevail. SP measurements have also regularly been made on the northern flank of the cone, on a west-east profile crossing the MFZ. Between 1981 and 1992 an enlargement and a shift of the MFZ to the east are evidenced. In particular, the eastern fissural axis trending N35°E could be related to the possible collapse of the east flank of the volcano. After a decrease between 1992 and 1997, the SP anomaly was enhanced again by the 1998 eruption. Short scale, about 250 m wide, 750 mV amplitude anomalies were superimposed on a large scale one, 2500 m wide, and about 250 mV in amplitude. For several years, continuous stations have been measuring the electric field along two directions, with a 20 s sampling, in order to record the genesis of SP signals associated with the volcanic activity. Oscillations belonging to the ULF band were evidenced several days before the 1988 eruption, some of them at 9 km from the summit. Their amplitude reached several tens mV/km. These oscillations sometimes present a phase lag from one station to another; they progressively shift towards the location of the future effusive vents. The polarisation of the oscillations is similar to the polarisation of longer SP variations (1 h period or more and are correlated with the structural anisotropy. Finally, during the last hours preceding the

  11. Self-potential changes associated with volcanic activity. Short-term signals associated with March 9, 1998 eruption on La Fournaise volcano (Reunion Island)

    Energy Technology Data Exchange (ETDEWEB)

    Zlotniki, J. [UMR6530, Clermont-Ferrand (France); Institut de Physique du Globe de Paris, Laboratoire de Geomagnetisme, Paris (France); Le Mouel, J. L. [Institut de Physique du Globe de Paris, Laboratoire de Geomagnetisme, Paris (France); Sasai, Y. [Tokyo Univ., Tokyo (Italy). Earthquake Research Institute; Yvetot, P.; Ardisson, M. H. [UMR6524, Laboratoire de Geophysique d' Orleans, Orleans (France)

    2001-04-01

    After six years of quietness La Fournaise volcano entered into activity on March 9, 1998. Fissures opened gradually downwards on the northern flank of the cone. Two cones, Kapor and Krafft built, from which lava poured until September 1998. Several other vents opened during this eruption. Mappings, surveys, and continuous recordings of the Self-Potential have been performed on the volcano for twenty years. SP mappings disclose the variability of large scale SP anomalies due to the modification of the hydrothermal system over some ten years. Most of the eruptions take place along a Main Fracture Zone (MFZ), in which ground water flows prevail. SP measurements have also regularly been made on the northern flank of the cone, on a west-east profile crossing the MFZ. Between 1981 and 1992 an enlargement and a shift of the MFZ to the east are evidenced. In particular, the eastern fissural axis trending N35{sup 0}E could be related to the possible collapse of the east flank of the volcano. After a decrease between 1992 and 1997, the SP anomaly was enhanced again by the 1998 eruption. Short scale, about 250 m wide, 750 mV amplitude anomalies were superimposed on a large scale one, 2500 m wide, and about 250 mV in amplitude. For several years, continuous stations have been measuring the electric field along two directions, with a 20 s sampling, in order to record the genesis of SP signals associated with the volcanic activity. Oscillations belonging to the ULF band were evidenced several days before the 1988 eruption, some of them at 9 km from the summit. Their amplitude reached several tens mV/km. These oscillations sometimes present a phase lag from summit. Their amplitude reached several tens mV/km. These oscillations sometimes present a phase lag from one station to another; they progressively shift towards the location of the future effusive vents. The polarisation of the oscillations is similar to the polarisation of longer SP variations (1 h period or more) and are

  12. Doubling the Spectrum of Time-Domain Induced Polarization: Removal of Harmonic Noise and Self-Potential Drift

    Science.gov (United States)

    Fiandaca, G.; Olsson, P. I.; Auken, E.; Larsen, J. J.; Maurya, P. K.; Dahlin, T.

    2015-12-01

    The extraction of spectral information in the inversion process of time-domain (TD) induced polarization (IP) data is changing the use of the IP method. Data interpretation is evolving from a qualitative description of the soil, able only to discriminate the presence of contrasts in chargeability parameters, towards a quantitative analysis of the investigated media, which allows soil-type characterization. Two major limitations restrict the extraction of the spectral information of TDIP data in the field: i) the difficulty of acquiring reliable early-time measurements, in the millisecond range and ii) the self-potential drift in the measured potentials distorting the shape of the late time IP decays, in the second range. For measuring at early-times, we developed a new method for removing the powerline noise contained in the data through a model-based approach, localizing the fundamental frequency of the powerline signal in the full-waveform IP recordings. By this, we cancel both the fundamental signal and its harmonics. This noise cancellation allows the use of earlier and narrower gates, down to a few milliseconds after the current turn-off. Even earlier gates can be measured but they will be inductively "contaminated" which we at present want to avoid. A proper removal of the self-potential drift present between the potential electrodes is essential for preserving the shape of the TD decays, especially for late times. Usually constant or linear drift-removal algorithms are used, but these algorithms fail in removing the background potentials due to the polarization of the electrodes previously used for current injection. We developed a drift-removal scheme that model the polarization effect and efficiently allows for preserving the shape of the IP decays. The removal of both the harmonic noise and self-potential drift allows for doubling the usable range of TDIP data to more than three decades in time (corresponding to three decays in frequency), and will

  13. Self-potential time series analysis in a seismic area of the Southern Apennines: preliminary results

    Directory of Open Access Journals (Sweden)

    V. Tramutoli

    1994-06-01

    Full Text Available The self-potential time series recorded during the period May 1991 - August 1992 by an automatic station, located in a seismic area of Southern Apennines, is analyzed. We deal with the spectral and the statistical features of the electrotellurie precursors: they can play a major role in the approach to seismic prediction. The time-dynamics of the experimental time series is investigated, the cyclic components and the time trends are removed. In particular we consider the influence of external noise, related to anthropic activities and meteoclimatic parameters, and pick out the anomalies from the residual series. Finally we show the preliminary results of the correlation between the anomalies in the time patterns of self-potential data and the earthquakes which occurred in the area.

  14. Self-potential, geoelectric and magnetotelluric studies in Italian active volcanic areas

    OpenAIRE

    Di Mai, R.; Mauriello, P.; Patella, D.; Petrillo, Z.; Piscitelli, S.; Siniscalchi, A.; Veneruso, M.

    1997-01-01

    We present the results of self-potential, geoelectric and magnetotelluric studies in Italian active volcanic areas as essential contributions both to structural modeling and to hazard evaluation. On Mt. Etna and Mt. Somma-Vesuvius complexes structural modeling was emphasized due to a lack of global information involving the whole apparatuses, at least from the electrical point of view. Hazard investigation was, instead, investigated with high resolution techniques on the island of Vulcano, wh...

  15. Wavelet analysis as a tool to characteriseand remove environmental noisefrom self-potential time series

    OpenAIRE

    Chianese, D.; Colangelo, G.; D'Emilio, M.; Lanfredi, M.; Lapenna, V.; Ragosta, M.; Macchiato, M. F.

    2004-01-01

    Multiresolution wavelet analysis of self-potential signals and rainfall levels is performed for extracting fluctuations in electrical signals, which might be addressed to meteorological variability. In the time-scale domain of the wavelet transform, rain data are used as markers to single out those wavelet coefficients of the electric signal which can be considered relevant to the environmental disturbance. Then these coefficients are filtered out and the signal is recovered by anti...

  16. Self-potential time series analysis in a seismic area of the Southern Apennines: preliminary results

    OpenAIRE

    Di Bello, G.; Lapenna, V.; Satriano, C.; Tramutoli, V.

    1994-01-01

    The self-potential time series recorded during the period May 1991 - August 1992 by an automatic station, located in a seismic area of Southern Apennines, is analyzed. We deal with the spectral and the statistical features of the electrotellurie precursors: they can play a major role in the approach to seismic prediction. The time-dynamics of the experimental time series is investigated, the cyclic components and the time trends are removed. In particular we consider the influence of external...

  17. Investigating the time-correlation properties in self-potential signals recorded in a seismic area of Irpinia, southern Italy

    International Nuclear Information System (INIS)

    Telesca, Luciano; Balasco, Marianna; Lapenna, Vincenzo

    2007-01-01

    Recent studies have shown that many natural phenomena are characterized by temporal fluctuations with long-range power-law correlations, suggesting a fractal geometry of the underlying dynamical system. The presence of power-law correlations are detected in four time series of self-potential signals, measured in a seismic area of southern Italy, by means of the Detrended Fluctuation Analysis (DFA), a method that permits the detection of long-range correlations in nonstationary time series. Results show scaling behaviour for all the signals recorded, indicating the presence of fractal features expressing a long-term correlation quantified by the numerical value of the scaling exponents. Our findings suggest a possible correlation between the earthquakes occurred in the area investigated and the relative maxima/minima of the mean and the standard deviation of the scaling exponents. Furthermore, the normalized average and standard deviation curves for all the signals tend to converge in correspondence with an earthquake

  18. Combined 2-D Electrical Resistivity and Self Potential Survey to Investigate the Pattern of the Watukosek Fault System Around the Lusi Eruption Site, Indonesia.

    Science.gov (United States)

    Mazzini, A.; Husein, A.; Mauri, G.; Lupi, M.; Hadi, S.; Kemna, A.

    2015-12-01

    The Lusi mud eruption is located in the Sidoarjo area, Indonesia and is continuously erupting hot mud since its birth in May 2006. A comprehensive combined electrical resistivity and self-potential (SP) survey was performed in the 7 km2 area inside the Lusi embankment that had been built to contain the erupted mud and to prevent flooding of the surrounding roads and settlements. The goal of the geophysical survey is to map the near-surface occurrence of the Watukosek fault system, upon which LUSI resides, delineate its spatial pattern and monitor its development. We completed six lines of measurements combining resistivity measurement using Wenner configuration and SP measurements using roll-along technique. Three subparallel lines were located either to the north and to the south of the main crater. Each line was approximately W-E oriented extending for ~1.26 km. The surveyed regions consist of dried mud breccia (containing clayey-silty-sandy admixture with clast up to ~ 10 cm in size). The thickness of the dry walkable mud is approximately 2-3 m and the deeper layer consist of water saturated mud that could be vulnerable to a liquefaction scenario in case of significant seismic activity in the region. The resistivity data were inverted into 2-D resistivity images with a maximum exploration depth of almost 200 m. The resistivity images consistently reveal a region of about 300 m in width (between 30-90 m depth) characterized by anomalous resistivities, which are lower than the value observed in the surounding area. The position of these anomalies is also supported by the SP data, which suggests that their origin is related to fluid flow path in the subsurface. Thus the combined resistivity and SP results allow inference of an improved model of the Watukosek fault system.

  19. P-type sp3-bonded BN/n-type Si heterodiode solar cell fabricated by laser-plasma synchronous CVD method

    International Nuclear Information System (INIS)

    Komatsu, Shojiro; Nagata, Takahiro; Chikyo, Toyohiro; Sato, Yuhei; Watanabe, Takayuki; Hirano, Daisuke; Takizawa, Takeo; Nakamura, Katsumitsu; Hashimoto, Takuya; Nakamura, Takuya; Koga, Kazunori; Shiratani, Masaharu; Yamamoto, Atsushi

    2009-01-01

    A heterojunction of p-type sp 3 -bonded boron nitride (BN) and n-type Si fabricated by laser-plasma synchronous chemical vapour deposition (CVD) showed excellent rectifying properties and proved to work as a solar cell with photovoltaic conversion efficiency of 1.76%. The BN film was deposited on an n-type Si (1 0 0) substrate by plasma CVD from B 2 H 6 + NH 3 + Ar while doping of Si into the BN film was induced by the simultaneous irradiation of an intense excimer laser with a pulse power of 490 mJ cm -2 , at a wavelength of 193 nm and at a repetition rate of 20 Hz. The source of dopant Si was supposed to be the Si substrate ablated at the initial stage of the film growth. The laser enhanced the doping (and/or diffusion) of Si into BN as well as the growth of sp 3 -bonded BN simultaneously in this method. P-type conduction of BN films was determined by the hot (thermoelectric) probe method. The BN/Si heterodiode with an essentially transparent p-type BN as a front layer is supposed to efficiently absorb light reaching the active region so as to potentially result in high efficiency.

  20. Seroprevalencia de Leptospira sp., Rickettsia sp. Ehrlichia sp. en trabajadores rurales del departamento de Sucre, Colombia Seroprevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp. in rural workers of Sucre, Colombia

    Directory of Open Access Journals (Sweden)

    Rodrigo Ríos

    2008-06-01

    Full Text Available Objective. Determinar la seroprevalencia de Leptospira sp., Rickettsia sp. y Ehrlichia sp. en trabajadores de áreas rurales del departamento de Sucre. Material y métodos. Se realizó un estudio escriptivo, prospectivo, de corte transversal, que pretendió determinar la seroprevalencia e Leptospira sp., Rickettsia sp. y Ehrlichia sp. en 90 trabajadores de áreas rurales del departamento de Sucre. Se estableció la presencia de anticuerpos séricos anti-IgM específicos anti-Leptospira por la técnica de ELISA indirecta. Para la determinación de Rickettsia sp. y Ehrlichia sp. se uso la técnica de inmunofluorescencia indirecta. Resultados. La población evaluada estaba compuesta por 27 (30% ordeñadores, 21 (23% jornaleros, 18 (20% profesionales del campo y 24 (27% que realizaban otras actividades. Ventidós (24% muestras resultaron positivas en alguna de las pruebas. De éstas, 12 (13,3% fueron positivas para Leptospira sp., 7 (7,8% para Rickettsia sp. y 3 (3,3% ara Ehrlichia sp. Conclusión. Este fue el primer estudio que se llevó a cabo en el departamento de Sucre y permitió demostrar que existe una prevalencia importante de Leptospira p.,Rickettsia sp. y Ehrlichia sp.. Los factores de riesgo ocupacional fueron factores determinantes en la seropositividad.Objective. To determine the seroprevalence of Leptospira sp., Rickettsia sp. and Ehrlichia sp. in agricultural workers of Sucre. Methods. A descriptive prospective cross-sectional study was conducted in ninety rural workers of Sucre. Presence of serum antibodies anti-IgM specific anti-Leptospira by indirect ELISA was established. For the determination of Rickettsia and Ehrlichia indirect inmunoflorescence was used. Results.The population was composed by 27 (30% milkers, 21 (23% day workers, 18 farm professionals (20% and 24 (26% workers in others activities. A total of 22 (24% samples were positive to some test. Twelve (13.3% were positive to Leptospira sp., seven (7.8% to Rickettsia sp

  1. 4-D imaging of seepage in earthen embankments with time-lapse inversion of self-potential data constrained by acoustic emissions localization

    Science.gov (United States)

    Rittgers, J. B.; Revil, A.; Planes, T.; Mooney, M. A.; Koelewijn, A. R.

    2015-02-01

    New methods are required to combine the information contained in the passive electrical and seismic signals to detect, localize and monitor hydromechanical disturbances in porous media. We propose a field experiment showing how passive seismic and electrical data can be combined together to detect a preferential flow path associated with internal erosion in a Earth dam. Continuous passive seismic and electrical (self-potential) monitoring data were recorded during a 7-d full-scale levee (earthen embankment) failure test, conducted in Booneschans, Netherlands in 2012. Spatially coherent acoustic emissions events and the development of a self-potential anomaly, associated with induced concentrated seepage and internal erosion phenomena, were identified and imaged near the downstream toe of the embankment, in an area that subsequently developed a series of concentrated water flows and sand boils, and where liquefaction of the embankment toe eventually developed. We present a new 4-D grid-search algorithm for acoustic emissions localization in both time and space, and the application of the localization results to add spatially varying constraints to time-lapse 3-D modelling of self-potential data in the terms of source current localization. Seismic signal localization results are utilized to build a set of time-invariant yet spatially varying model weights used for the inversion of the self-potential data. Results from the combination of these two passive techniques show results that are more consistent in terms of focused ground water flow with respect to visual observation on the embankment. This approach to geophysical monitoring of earthen embankments provides an improved approach for early detection and imaging of the development of embankment defects associated with concentrated seepage and internal erosion phenomena. The same approach can be used to detect various types of hydromechanical disturbances at larger scales.

  2. Self-potential, geoelectric and magnetotelluric studies in Italian active volcanic areas

    Directory of Open Access Journals (Sweden)

    A. Siniscalchi

    1997-06-01

    Full Text Available We present the results of self-potential, geoelectric and magnetotelluric studies in Italian active volcanic areas as essential contributions both to structural modeling and to hazard evaluation. On Mt. Etna and Mt. Somma-Vesuvius complexes structural modeling was emphasized due to a lack of global information involving the whole apparatuses, at least from the electrical point of view. Hazard investigation was, instead, investigated with high resolution techniques on the island of Vulcano, where intense unrest phenomena have long been recorded.

  3. SP. Pescado

    Directory of Open Access Journals (Sweden)

    Renato Gendre

    2003-12-01

    Full Text Available Nell'occhiello di un articolo dal titolo Il Peru dei de[Jini rosa e de/la grande pioggia si legge: "da una partenza  in aereo al «pescado»  che ti  sfamera."1 Questa parola spagnola, giustamente chiusa tra caporali, a noi pare molto interes­ sante, perche, nonostante l'apparenza, non ha nulla da spartire sotto i1 profilo se­ mantico con l'it. pescato. lnfatti, tutti i piu importanti dizionari della lingua italiana, di ieri e di oggi, etimologici e non 2, registrano  accanto a pescata,  ii lemma pescato, 3 ma lo spiegano come "quantita di pesce catturato nel corso di una battuta o di una stagione di pesca",4 mentre lo sp. pescado  indica i1 "pesce (solo nel senso di: pesGe pescato da mangiare [...]".s

  4. 3D Hydraulic tomography from joint inversion of the hydraulic heads and self-potential data. (Invited)

    Science.gov (United States)

    Jardani, A.; Soueid Ahmed, A.; Revil, A.; Dupont, J.

    2013-12-01

    Pumping tests are usually employed to predict the hydraulic conductivity filed from the inversion of the head measurements. Nevertheless, the inverse problem is strongly underdetermined and a reliable imaging requires a considerable number of wells. We propose to add more information to the inversion of the heads by adding (non-intrusive) streaming potentials (SP) data. The SP corresponds to perturbations in the local electrical field caused directly by the fow of the ground water. These SP are obtained with a set of the non-polarising electrodes installed at the ground surface. We developed a geostatistical method for the estimation of the hydraulic conductivity field from measurements of hydraulic heads and SP during pumping and injection experiments. We use the adjoint state method and a recent petrophysical formulation of the streaming potential problem in which the streaming coupling coefficient is derived from the hydraulic conductivity allowed reducing of the unknown parameters. The geostatistical inverse framework is applied to three synthetic case studies with different number of the wells and electrodes used to measure the hydraulic heads and the streaming potentials. To evaluate the benefits of the incorporating of the streaming potential to the hydraulic data, we compared the cases in which the data are coupled or not to map the hydraulic conductivity. The results of the inversion revealed that a dense distribution of electrodes can be used to infer the heterogeneities in the hydraulic conductivity field. Incorporating the streaming potential information to the hydraulic head data improves the estimate of hydraulic conductivity field especially when the number of piezometers is limited.

  5. Use of geophysical methods to characterize groundwater in karstic rocks near Puerto Morelos, Yucatan Peninsula, Mexico

    Science.gov (United States)

    cerda Garcia, C. G.; Carpenter, P. J.; Leal-Bautista, R. M.

    2017-12-01

    Geophysical surveys were used to determine the depth of the freshwater/saltwater interface and groundwater preferential flow pathways along the Ruta de los Cenotes, near Puerto Morelos (northeast part of the Yucatán peninsula). The Yucatán Peninsula is a limestone platform that allows quick recharge of the aquifer, the main supply of water for this region. The water in the aquifer is divided into freshwater and saltwater zones. A Schlumberger resistivity sounding along the road near one cenote suggests the water table is 5 meters deep and the freshwater/saltwater interface is 38 meters deep. A time-domain electromagnetic (TEM) sounding suggests the freshwater/saltwater interface is 45 meters deep. The depth of the interface determines the volume of fresh water available. Preferential flow pathways in the vadose and saturated zones are karst conduits where groundwater percolates downward in the vadose zone. These were identified using resistivity profiling and spontaneous self-potential (SP) geophysical methods. Interpretation of SP profile Line SP1, located 3 m south of the cenote, suggests two fractures, which appear to extend south as far as SP profile Line SP2, 15 m south of the cenote; both lines are parallel to each other. SP anomalies suggest water flow along these fractures. The use of noninvasive geophysical methods, specifically SP, resistivity and TEM are useful for exploring the karst system in the Yucatán peninsula.

  6. Biochemical and physicochemical analysis of fish protein isolate recovered from red snapper (Lutjanus sp.) by-product using isoelectric solubilization/precipitation method

    Science.gov (United States)

    Pramono, H.; Pujiastuti, D. Y.; Sahidu, A. M.

    2018-04-01

    The effect of acid- and alkali-process on biochemical and physicochemical characteristics of fish protein isolate from red snapper (Lutjanus sp) by-product was evaluated. Protein recovered by alkali process (16.79%) was higher compared to acid process (13.75%). Reduction of lipid content and total volatile basic nitrogen (TVB-N) exhibited in both treatments indicated both process improved fish protein isolate recovered from red snapper by-product. In addition, the increasing of water holding capacity and oil binding capacity were observed. However, high peroxide value of fish protein isolate was showed in both treatment. This finding indicated that acid and alkali process can be used as a useful method to recover proteins from red snapper by-product. Alkali process gave a protein isolate with better overall quality compared to acid process.

  7. Pengendalian Hayati Penyakit Layu Fusarium Pisang (Fusarium Oxysporum F.sp. Cubense) dengan Trichoderma SP.

    OpenAIRE

    Sudirman, Albertus; Sumardiyono, Christanti; Widyastuti, Siti Muslimah

    2011-01-01

    The aim of this research was to study the inhibiting ability of Trichoderma sp. to control fusarium wilt of banana in greenhouse condition. The experiments consisted of the antagonism test between Trichoderma sp. and Fusarium oxysporum f.sp. cubense (Foc) in vitro using dual culture method and glass house experiment which was arranged in 3×3 Factorial Complete Randomized Design. First factor of the latter experiment was the dose of Trichoderma sp. culture (0, 25, and 50 g per polybag), second...

  8. Selective C(sp2)-C(sp) bond cleavage: the nitrogenation of alkynes to amides.

    Science.gov (United States)

    Qin, Chong; Feng, Peng; Ou, Yang; Shen, Tao; Wang, Teng; Jiao, Ning

    2013-07-22

    Breakthrough: A novel catalyzed direct highly selective C(sp2)-C(sp) bond functionalization of alkynes to amides has been developed. Nitrogenation is achieved by the highly selective C(sp2)-C(sp) bond cleavage of aryl-substituted alkynes. The oxidant-free and mild conditions and wide substrate scope make this method very practical. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. First report of Anisakis sp. in Epinephelus sp. in East Indonesia

    OpenAIRE

    Annytha Ina Rohi Detha; Diana Agustiani Wuri; Julianty Almet; Yuni Riwu; Christin Melky

    2018-01-01

    Objective: The present research was conducted to identify the prevalence of Anisakis sp. as fish-borne zoonoses in Epinephelus sp. in territorial waters of East Nusa Tenggara, Indonesia. Materials and methods: A total of 50 fish (Epinephelus sp.) were collected from Kupang Fish Market in East Nusa Tenggara. Identification of Anisakis sp. was performed based on morphological observations considering shape of ventriculus, boring tooth, and mucron using binocular microscope. Results: Prev...

  10. Optimization of pH and nitrogen for enhanced hydrogen production by Synechocystis sp. PCC 6803 via statistical and machine learning methods.

    Science.gov (United States)

    Burrows, Elizabeth H; Wong, Weng-Keen; Fern, Xiaoli; Chaplen, Frank W R; Ely, Roger L

    2009-01-01

    The nitrogen (N) concentration and pH of culture media were optimized for increased fermentative hydrogen (H(2)) production from the cyanobacterium, Synechocystis sp. PCC 6803. The optimization was conducted using two procedures, response surface methodology (RSM), which is commonly used, and a memory-based machine learning algorithm, Q2, which has not been used previously in biotechnology applications. Both RSM and Q2 were successful in predicting optimum conditions that yielded higher H(2) than the media reported by Burrows et al., Int J Hydrogen Energy. 2008;33:6092-6099 optimized for N, S, and C (called EHB-1 media hereafter), which itself yielded almost 150 times more H(2) than Synechocystis sp. PCC 6803 grown on sulfur-free BG-11 media. RSM predicted an optimum N concentration of 0.63 mM and pH of 7.77, which yielded 1.70 times more H(2) than EHB-1 media when normalized to chlorophyll concentration (0.68 +/- 0.43 micromol H(2) mg Chl(-1) h(-1)) and 1.35 times more when normalized to optical density (1.62 +/- 0.09 nmol H(2) OD(730) (-1) h(-1)). Q2 predicted an optimum of 0.36 mM N and pH of 7.88, which yielded 1.94 and 1.27 times more H(2) than EHB-1 media when normalized to chlorophyll concentration (0.77 +/- 0.44 micromol H(2) mg Chl(-1) h(-1)) and optical density (1.53 +/- 0.07 nmol H(2) OD(730) (-1) h(-1)), respectively. Both optimization methods have unique benefits and drawbacks that are identified and discussed in this study. (c) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009.

  11. Wavelet analysis as a tool to characteriseand remove environmental noisefrom self-potential time series

    Directory of Open Access Journals (Sweden)

    M. Ragosta

    2004-06-01

    Full Text Available Multiresolution wavelet analysis of self-potential signals and rainfall levels is performed for extracting fluctuations in electrical signals, which might be addressed to meteorological variability. In the time-scale domain of the wavelet transform, rain data are used as markers to single out those wavelet coefficients of the electric signal which can be considered relevant to the environmental disturbance. Then these coefficients are filtered out and the signal is recovered by anti-transforming the retained coefficients. Such methodological approach might be applied to characterise unwanted environmental noise. It also can be considered as a practical technique to remove noise that can hamper the correct assessment and use of electrical techniques for the monitoring of geophysical phenomena.

  12. Draft genome sequences of six neonatal meningitis-causing escherichia coli isolates (SP-4, SP-5, SP-13, SP-16, SP-46, and SP-65)

    Science.gov (United States)

    Neonatal meningitis Escherichia coli isolates (SP-4, SP-5, SP-13, SP-16, SP-46, and SP-65) were recovered from infants in the Netherlands from 1989 to 1997. Here, we report the draft genome sequences for these six E. coli isolates, which are currently being used to validate food safety processing te...

  13. Exploring the Effect of Media, Salinity and Clay on the Thermoelectric Coupling Coefficient in Self-Potential Data

    Science.gov (United States)

    Meyer, C. D.; Revil, A.

    2014-12-01

    Self-potential is a non-invasive, passive geophysical technique with applications ranging from imaging oil and gas reservoirs to identifying preferential flow paths in earthen embankments. Several cross-coupled flow phenomena contribute to self-potential data, and there is a need to further quantify these various sources to enable better resolution and quantification of self-potential models. Very little research has been done to constrain thermoelectric source mechanisms that contribute to self-potential signals. A laboratory experiment has been designed to investigate the thermoelectric coupling coefficient (CTE) that relates the voltage change per degree centigrade (V/°C) in porous media. This study focuses on a sand tank experiment using a saturated silica sand. To isolate the temperature gradient dependence of self-potential measurements, no hydraulic gradient is applied to the tank, eliminating the streaming potential component of source current. Self-potential and temperature data are recorded while reservoirs of hot and cold water are established on opposite ends of the tank in order to generate thermoelectric source currents. Various thermal gradients ranging from 0 °C to 80 °C over 20 cm are examined for various salinities (10-3M- 1M NaCl), sand grain sizes and clay content to investigate influences on CTE. A short-duration contact of non-polarizing (Pb/PbCl) electrodes is implemented to minimize temperature drift of electrodes during the experiment. Surface self-potential and temperature measurements are made in 30 minute intervals. Initial measurements have revealed non-linear effects, including a decreased CTE as temperature gradient bounds approach 0 °C.

  14. Investigating correlations between earthquakes and extreme eventsin self-potential data recorded in a seismicarea of Southestern Appennine Chain (Italy

    Directory of Open Access Journals (Sweden)

    V. Lapenna

    2004-06-01

    Full Text Available The Normalized Wavelet Cross-Correlation Function (NWCCF was used to study correlations between the series of extreme events in self-potential data and earthquakes, both modelled as stochastic point processes. This method gives objective results, robust to the presence of nonstationarities that often affect observational time series. Furthermore, the NWCCF identifies the timescales involved in the cross-correlated behaviour between two point processes. In particular, we analyzed the cross-correlation between the sequence of extreme events in selfpotential data measured at the monitoring station Tito, located in a seismic area of Southern Italy, and the series of earthquakes which occurred in the same area during 2001. To evaluate the influence of rain on the dynamics of geoelectrical variations, we applied the same approach between the selected extreme values and the rain data. We find that the anomalous geoelectrical values seem to cross-correlate with the rain at short and intermediate timescales (t< 500 h, while they significantly cross-correlate only with earthquakes (M = 2.5 at long timescales (t> 500 h.

  15. A rapid genotyping method for an obligate fungal pathogen, Puccinia striiformis f.sp. tritici, based on DNA extraction from infected leaf and Multiplex PCR genotyping

    Directory of Open Access Journals (Sweden)

    Enjalbert Jérôme

    2011-07-01

    Full Text Available Abstract Background Puccinia striiformis f.sp. tritici (PST, an obligate fungal pathogen causing wheat yellow/stripe rust, a serious disease, has been used to understand the evolution of crop pathogen using molecular markers. However, numerous questions regarding its evolutionary history and recent migration routes still remains to be addressed, which need the genotyping of a large number of isolates, a process that is limited by both DNA extraction and genotyping methods. To address the two issues, we developed here a method for direct DNA extraction from infected leaves combined with optimized SSR multiplexing. Findings We report here an efficient protocol for direct fungal DNA extraction from infected leaves, avoiding the costly and time consuming step of spore multiplication. The genotyping strategy we propose, amplified a total of 20 SSRs in three Multiplex PCR reactions, which were highly polymorphic and were able to differentiate different PST populations with high efficiency and accuracy. Conclusion These two developments enabled a genotyping strategy that could contribute to the development of molecular epidemiology of yellow rust disease, both at a regional or worldwide scale.

  16. Bioactivity of a Novel Glycolipid Produced by a Halophilic Buttiauxella sp. and Improving Submerged Fermentation Using a Response Surface Method

    Directory of Open Access Journals (Sweden)

    Abdolrazagh Marzban

    2016-09-01

    Full Text Available An antimicrobial glycolipid biosurfactant (GBS, extracted and identified from a marine bacterium, was studied to inhibit pathogenic microorganisms. Production of the GBS was optimized using a statistical method, a response surface method (RSM with a central composite design (CCD for obtaining maximum yields on a cost-effective substrate, molasses. The GBS-producing bacterium was identified as Buttiauxella Species in terms of biochemical and molecular characteristics. This compound showed a desirable antimicrobial activity against some pathogens such as E. coli, Bacillus subtilis, Bacillus cereus, Candida albicans, Aspergilus niger, Salmonella enterica. The rheological studies described the stability of the GBS at high values in a range of pH (7–8, temperature (20–60 and salinity (0%–3%. The statistical optimization of GBS fermentation was found to be pH 7, temperature 33 °C, Peptone 1%, NaCl 1% and molasses 1%. The potency of the GBS as an effective antimicrobial agent provides evidence for its use against food and human pathogens. Moreover, favorable production of the GBS in the presence of molasses as a cheap substrate and the feasibility of pilot scale fermentation using an RSM method could expand its uses in food, pharmaceutical products and oil industries.

  17. Production of enzymes by a newly isolated Bacillus sp. TMF-1 in solid state fermentation on agricultural by-products: The evaluation of substrate pretreatment methods.

    Science.gov (United States)

    Salim, Abdalla Ali; Grbavčić, Sanja; Šekuljica, Nataša; Stefanović, Andrea; Jakovetić Tanasković, Sonja; Luković, Nevena; Knežević-Jugović, Zorica

    2017-03-01

    Study on potential of different agro-industrial waste residues for supporting the growth of newly isolated Bacillus sp. TMF-1 strain under solid-state fermentation (SSF) was conducted aiming to produce several industrially valuable enzymes. Since the feasibility of the initial study was confirmed, further objectives included evaluation of several pretreatments of the studied agricultural by-products (soybean meal, sunflower meal, maize bran, maize pericarp, olive oil cake and wheat bran) on the microbial productivity as means of enhancing the yields of produced proteases, α-amylases, cellulases and pectinases. Among acid/alkaline treatment, ultrasound and microwave assisted methods, chemical treatments superiorly affected most of the studied substrates. Highest yields of produced proteases (50.5IUg -1 ) and α-amylases (50.75IUg -1 ) were achieved on alkaline treated corn pericarp. Alkaline treatment also promoted the secretion of cellulases on maize bran (1.19IUg -1 ). Highest yield of pectinases was obtained on untreated soybean meal (64.90IUg -1 ). Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. Evaluation of the chromium contamination at Ribeirão dos Bagres, Franca (SP), Brazil, by the 210Pb method

    International Nuclear Information System (INIS)

    Matamet, F.R.M.; Bonotto, D.M.

    2013-01-01

    This paper presents a chronological study focusing the use of 210 Pb in four sediment cores collected at Ribeirão dos Bagres in the region of Franca, São Paulo State, Brazil. When evaluating the sedimentation rate by using the 210 Pb method, it was possible to monitor historical changes occurring in the concentrations of chromium present in the sediments. In order to do so, several specific parameters were determined in sediments, as chromium concentration, major oxides, organic matter, grain size, and chemical and physical–chemical parameters of waters. The sediments possessed a Cr concentration range of 0.83–26.25 mg/kg that is below the world's average of shales. The sedimentation rates obtained from the 210 Pb analysis were between 0.07 and 0.77 g cm −2 year −1 , while the linear sedimentation rate was from 0.6 to 3.2 cm/year. The adoption of the CIC (Constant Initial Concentration) 210 Pb model allowed characterize older sediments, aged 177 years, and younger sediments, aged 4 years. The organic matter in the sediments was more abundant in the first few centimeters of practically all profiles, and the analysis of oxides showed that silica is the major constituent of the sediments, in which there is predominance of sand and silt. In addition, the analysis of the physical and chemical parameters of waters indicated that they are suitable for navigation and natural harmony landscape. - Highlights: • Application of natural radionuclides in an important industrial center devoted to the production of leather for manufacturing shoes. • Study of a well-known site of environmental Cr contamination. • Use of the Pb-210 chronological CIC model to provide an approach of the historical Cr contamination in the region

  19. DISTRIBUSI Solen sp DI PERAIRAN KABUPATEN BANGKALAN

    Directory of Open Access Journals (Sweden)

    Eva Ari Wahyuni

    2016-03-01

    Full Text Available DISTRIBUTION OF Solen sp IN BANGKALAN WATERSSolen sp potential needs to be developed on the island of Madura, particularly in Bangkalan. Solen sp utilization has increased which has the potential to overfishing. Therefore, this study aims to determine the density of Solen sp and their ecology in the waters Modung village, Modung District, Bangkalan. The experiment was conducted in April 2015 using the descriptive method. The materials used include Solen sp and physico-chemical parameters of the environment (temperature, salinity, pH, and substrate. The analyzes were conducted at the Laboratory of Marine Science, Department of Marine Sciences, Trunojoyo University of Madura by using the tool grabsampler, sieveshaker, and pipetting with gravimetric method. The analysis shows the range of values of temperature between 29-300C, salinity between 31-32 ppt, pH were 7.9-8.0 and the type of substrate in the form of sandy mud, as well as the density of Solen sp from 8-10 individuals/m2. All measurement results indicate normal conditions and in accordance with the sea water quality standard for marine life, which can be a suitable habitat for the growth and development of Solen sp. This condition is thought to affect the density of Solen sp.Keywords: Bangkalan, density, distribution, Solen sp, substrate.ABSTRAKPotensi Solen sp perlu dikembangkan di pulau Madura, khususnya di Kabupaten Bangkalan. Pemanfaatan Solen sp mengalami peningkatan sehingga berpotensi overfishing. Untuk itu, penelitian ini bertujuan untuk mengetahui kepadatan Solen sp dan ekologinya di perairan desa Modung, Kecamatan Modung, Kabupaten Bangkalan. Penelitian dilaksanakan pada bulan April 2015 dengan metode deskriptif. Materi dan bahan yang digunakan diantaranya Solen sp dan parameter fisika-kimia lingkungan (suhu, salinitas, pH, dan substrat. Analisa dilakukan di Laboratorium Ilmu Kelautan, Program studi/Jurusan Ilmu Kelautan Universitas Trunojoyo Madura dengan menggunakan alat

  20. Silencing the SpMPK1, SpMPK2, and SpMPK3 Genes in Tomato Reduces Abscisic Acid—Mediated Drought Tolerance

    Directory of Open Access Journals (Sweden)

    Yan Liang

    2013-11-01

    Full Text Available Drought is a major threat to agriculture production worldwide. Mitogen-activated protein kinases (MAPKs play a pivotal role in sensing and converting stress signals into appropriate responses so that plants can adapt and survive. To examine the function of MAPKs in the drought tolerance of tomato plants, we silenced the SpMPK1, SpMPK2, and SpMPK3 genes in wild-type plants using the virus-induced gene silencing (VIGS method. The results indicate that silencing the individual genes or co-silencing SpMPK1, SpMPK2, and SpMPK3 reduced the drought tolerance of tomato plants by varying degrees. Co-silencing SpMPK1 and SpMPK2 impaired abscisic acid (ABA-induced and hydrogen peroxide (H2O2-induced stomatal closure and enhanced ABA-induced H2O2 production. Similar results were observed when silencing SpMPK3 alone, but not when SpMPK1 and SpMPK2 were individually silenced. These data suggest that the functions of SpMPK1 and SpMPK2 are redundant, and they overlap with that of SpMPK3 in drought stress signaling pathways. In addition, we found that SpMPK3 may regulate H2O2 levels by mediating the expression of CAT1. Hence, SpMPK1, SpMPK2, and SpMPK3 may play crucial roles in enhancing tomato plants’ drought tolerance by influencing stomatal activity and H2O2 production via the ABA-H2O2 pathway.

  1. Hydrodynamic parameters estimation from self-potential data in a controlled full scale site

    Science.gov (United States)

    Chidichimo, Francesco; De Biase, Michele; Rizzo, Enzo; Masi, Salvatore; Straface, Salvatore

    2015-03-01

    A multi-physical approach developed for the hydrodynamic characterization of porous media using hydrogeophysical information is presented. Several pumping tests were performed in the Hydrogeosite Laboratory, a controlled full-scale site designed and constructed at the CNR-IMAA (Consiglio Nazionale delle Ricerche - Istituto di Metodologia per l'Analisi Ambientale), in Marsico Nuovo (Basilicata Region, Southern Italy), in order to obtain an intermediate stage between laboratory experiments and field survey. The facility consists of a pool, used to study water infiltration processes, to simulate the space and time dynamics of subsurface contamination phenomena, to improve and to find new relationship between geophysical and hydrogeological parameters, to test and to calibrate new geophysical techniques and instruments. Therefore, the Hydrogeosite Laboratory has the advantage of carrying out controlled experiments, like in a flow cell or sandbox, but at field comparable scale. The data collected during the experiments have been used to estimate the saturated hydraulic conductivity ks [ms-1] using a coupled inversion model working in transient conditions, made up of the modified Richards equation describing the water flow in a variably saturated porous medium and the Poisson equation providing the self-potential ϕ [V], which naturally occurs at points of the soil surface owing to the presence of an electric field produced by the motion of underground electrolytic fluids through porous systems. The result obtained by this multi-physical numerical approach, which removes all the approximations adopted in previous works, makes a useful instrument for real heterogeneous aquifer characterization and for predictive analysis of its behavior.

  2. QTL list: SpRg-7 [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

    Lifescience Database Archive (English)

    Full Text Available entage concerning shoot regeneration 2 ME10-141 ... Chr07 19.51 6.84 ... 10.1186/1471-2229-11-140 22014149 ... QT73653 Solanum lycopersicum Solanaceae SpRg-7 In vitro plant regeneraion bud perc

  3. Assessment of geothermal energy potential by geophysical methods: Nevşehir Region, Central Anatolia

    Science.gov (United States)

    Kıyak, Alper; Karavul, Can; Gülen, Levent; Pekşen, Ertan; Kılıç, A. Rıza

    2015-03-01

    In this study, geothermal potential of the Nevşehir region (Central Anatolia) was assessed by using vertical electrical sounding (VES), self-potential (SP), magnetotelluric (MT), gravity and gravity 3D Euler deconvolution structure analysis methods. Extensive volcanic activity occurred in this region from Upper Miocene to Holocene time. Due to the young volcanic activity Nevşehir region can be viewed as a potential geothermal area. We collected data from 54 VES points along 5 profiles, from 28 MT measurement points along 2 profiles (at frequency range between 320 and 0.0001 Hz), and from 4 SP profiles (total 19 km long). The obtained results based on different geophysical methods are consistent with each other. Joint interpretation of all geological and geophysical data suggests that this region has geothermal potential and an exploration well validated this assessment beyond doubt.

  4. In situ analysis of coal from single electrode resistance, self-potential and gamma-ray logs

    International Nuclear Information System (INIS)

    Kayal, J.R.

    1981-01-01

    Single electrode resistance, self-potential and gamma-ray logging have been carried out in North Karanpura, West Bokaro and Jharia coalfields of Gondwana basin in Eastern India. Correlation of these geophysical logs is found to be very useful in locating the coal beds, determining their accurate depths and thickness and approximate quality. Coal seams have been detected as very high resistive formations compared to sandstone/shale which are interbedded in the coal basin. High or low self-potential values are obtained against the coal beds depending on the borehole fluid conditions. Burnt coals (Jhama) are characterised as highly conductive beds. Gamma ray logs have been effectively used alongwith electrical logs for correlation and identification of coal seams. Further analysis of gamma-ray log data determines a linear relationship with ash content of coal. (author)

  5. A new multiparametric geophysical station to detect self-potential and seismometric signals at Tito site (Southern Italy)

    Energy Technology Data Exchange (ETDEWEB)

    Balasco, M.; Lapenna, V.; Chianese, D.; Gallipoli, M. R. [Consiglio Nazionale delle Ricerche, Ist. di Metodologie Avanzate di Analisi Ambientale, Tito Scalo, PZ (Italy); Di Bello, G. [Potenza Universita' della Basilicata, Potenza (Italy). Dipt. di Ingegneria e Fisica dell' Ambiente

    2001-04-01

    In this work are presented the main features of a new multiparametric station able to jointly detect self-potential and seismometric signals in a seismic active area of Southern Italy. The new station has been designed and installed at the Tito Laboratories of National Research Council (Italy) that are located in the Southern Apennines, one of the most tectonically active areas of the whole Mediterranean. It combines advanced technologies for data acquisition with robust statistical techniques to pick out extreme events from self-potential recordings. The completely automatic station is equipped with electrical and seismometric sensors (16 channels, A/D 24 bit, sampling rate of 0.25 Hz, range dynamics of 133 dB). After a preliminary filtering procedure, mainly devoted to removing any influence of meteo-climatic conditions and/or cultural electrical noise, it was evaluated the performance of the new monitoring station investigating the possible correlation between anomalous patterns of the self-potential signals and local seismic activity. Objective criteria and robust statistical tools have been applied to identify extreme events in electrical measurements and to select the earthquakes that may be responsible for strain effects at the measuring point. The short period of the measuring activity does not allow anybody to give firm conclusions, however the first results encourage everybody to continue the monitoring activity by increasing the number of remote stations and improving the use of statistical packages for data processing. It was identified a well based monitoring strategy that in the near future could be useful to better understand the possible correlation between anomalous self-potential signals and local seismic activity.

  6. Pengendalian Hayati Penyakit Layu Fusarium Pisang (Fusarium oxysporum f.sp. cubense dengan Trichoderma sp.

    Directory of Open Access Journals (Sweden)

    Albertus Sudirman

    2011-07-01

    Full Text Available The aim of this research was to study the inhibiting ability of Trichoderma sp. to control fusarium wilt of banana in greenhouse condition. The experiments consisted of the antagonism test between Trichoderma sp. and Fusarium oxysporum f.sp. cubense (Foc in vitro using dual culture method and glass house experiment which was arranged in 3×3 Factorial Complete Randomized Design. First factor of the latter experiment was the dose of Trichoderma sp. culture (0, 25, and 50 g per polybag, second factor was time of Trichoderma culture application (2 weeks before Foc inoculation, at same time with Foc inoculation and 2 weeks after Foc inoculation. Trichoderma sp. was cultured in mixed rice brand and chaff medium. The disease intensity was observed with scoring system of wilting leaves (0–4. The results showed that Trichoderma sp. was antagonistic against Foc in vitro and inhibited 86% of Foc colony development. Mechanism of antagonism between Trichoderma sp. and Foc was hyperparasitism. Trichoderma hyphae coiled around Foc hyphae. Lysis of Foc hyphae was occurred at the attached site of Trichoderma hyphae on Foc hyphae. Added banana seedling with Trichoderma sp. Culture reduced disease intensity of Fusarium wilt. Suggested dose of Trichoderma culture application in glass house was 25 g/polybag, given at the same time with Foc inoculation. Penelitian ini bertujuan untuk mengetahui kemampuan Trichoderma sp. untuk pengendalian penyakit layu fusarium pisang di rumah kaca. Penelitian meliputi pengujian daya hambat Fusarium oxysporum f.sp. cubense (Foc in vitro dan kemampuan menekan intensitas penyakit di rumah kaca. Penelitian in vitro meliputi uji antagonisme dan mekanismenya yang dilakukan secara dual culture. Uji pengaruh Trichoderma sp. terhadap penyakit layu Fusarium dilakukan di rumah kaca dengan Rancangan Acak Lengkap Faktorial. Faktor pertama adalah dosis biakan Trichoderma sp., dengan tiga aras (0, 25, 50 g/per bibit dalam polibag. Faktor kedua

  7. Penggunaan Streptomyces sp. Sebagai Biokontrol Penyakit Layu Pada Tanaman Cabai Merah (Capsicum annuum L. yang Disebabkan Oleh Fusarium oxysporum f.sp. capsici

    Directory of Open Access Journals (Sweden)

    ANINDA OKTAVIA RAHARINI

    2014-01-01

    Full Text Available A research has been conducted to find out Streptomyces bacteria at Bukit Jimbaran, to inhibitionpotency of Streptomyces sp. to pathogenic fungi Fusarium oxysporum f.sp. capsici, and to find outantifungal activity of Streptomyces filtrate to F.oxysporum f.sp. capsici in chili (Capsicum annuumL. plants. Streptomyces sp. isolation was done by platting method with selective media YMA (ISP4.Identification of Streptomyces sp. used Bergey’s book entitled Manual Determinative Bacteriology.Test inhibition against F.oxysporum f.sp. capsici and in vivo test used by dying the roots of the chili(C.annuum L. plant with F.oxysporum f.sp. capsici and after 30 seconds the roots were dying withStreptomyces sp. culture, furthermore sterile soil on polybag watered by F.oxysporum f.sp. capsicispore and Streptomyces sp. culture at the same time. The result found five isolates Streptomyces sp.with different morphological. The antagonis test showed Streptomyces sp. 4 had ability (82% againstFusarium, Streptomyces sp.1 (72%, Streptomyces sp.2 (64%, Streptomyces sp.3 (76%, andStreptomyces sp. 5 (32%. All Streptomyces suppressed the growth of Fusarium on chili plants inglass house (p<0,05. Streptomyces sp.4 suppressed Fusarium wilt disease in chili from 80% in controlto 8%.

  8. Ovos de Toxocara sp. e larvas de Ancylostoma sp. em praça pública de Lavras, MG Toxocara sp. eggs and Ancylostoma sp. larva in public parks, Brazil

    Directory of Open Access Journals (Sweden)

    Antônio Marcos Guimarães

    2005-04-01

    Full Text Available Larva migrans visceral e cutânea são zoonoses parasitárias causadas pela infecção da larva de Toxocara sp. e Ancylostoma sp., respectivamente. O objetivo do estudo foi verificar a contaminação por ovos de Toxocara sp. e ovos e larvas de Ancylostoma sp. em amostras de solos coletadas de praças públicas e de áreas de recreação infantil de Lavras, Estado de Minas Gerais, por meio da técnica de centrífugo-flutuação e do método de Baermann. A ocorrência de ovos de Toxocara sp. e, ovos e larvas de Ancylostoma sp. foi observada em 69,6% (16/23 das amostras de solo coletadas de praças públicas. A contaminação somente por ovos de Ancylostoma sp. em amostras de solo coletadas em escolas/creches foi de 22,2% (4/18. A percentagem de amostras de areia coletadas de escolas/creches contaminadas somente com larvas de Ancylostoma sp. foi de 11,1% (2/18. Praças públicas são as áreas com maior risco potencial de infecção por Toxocara sp. e Ancylostoma sp. Exame coproparasitológico realizado em 174 amostras de fezes de cães observou 58% e 23%, respectivamente, com ovos de Ancylostoma sp. e Toxocara sp.Visceral and cutaneous larva migrans are parasitic zoonoses caused by the infection of larval nematodes Toxocara sp. and Ancylostoma sp. respectively. The objective of this study was to investigate the contamination by Toxocara sp. eggs and Ancylostoma sp. eggs and larva of soil samples collected from public parks and children's playground areas in state of Minas Gerais, Brazil, using both Baermann's method and centrifugal flotation technique. Toxocara sp. and Ancylostoma sp. eggs were observed in soil samples collected from public squares in 17.4% (4/23 and 69.6 (16/23 respectively. In schools and child day care settings the contamination by Ancylostoma sp. larva in sand samples was 11.1% (2/18. Public parks are settings of more potential risk of Toxocara sp. eggs and Ancylostoma sp. infection. Stool parasitology testing of 174 stool

  9. Fatty acid composition of Spirulina sp., Chlorella sp. and Chaetoceros sp. microalgae and introduction as potential new sources to extinct omega 3 and omega 6

    Directory of Open Access Journals (Sweden)

    Homan Gorjzdadeh

    2016-05-01

    Full Text Available Background: This study was carried out to determine the oil fatty acids from two special species of microalgae; Spirulina sp.,Chlorella sp. and also Chaetoceros sp. collected from Bahmanshir River. Materials and Methods: Sampling of microalgae Chaetoceros sp. from Bahmanshir River was under taken using bottle samplers during spring season of 2013. Microalgae Spirulina sp. and Chlorella sp. were supplied from Shrimp Research Institute of Iran in Bushehr Province. Samples then were cultured under controlled laboratory conditions and mass culture for 100 liters was undertaken. Isolation of microalgae species from water of cultured media was carried out using filtration and centrifugation methods. The fatty acid compositions were determined by Gas – FID chromatography. Results: Results showed that regarding Saturated Fatty Acids (SFA obtained from purified culture of Chaetoceros sp., Spirulina sp. and Chlorella sp. the maximum amount of total fatty acids were belonged to palmitic acids (C16:0 with 15.21%, 30.1% and 25.17% of total fatty acids  respectively. Analysis of Mono Unsaturated Fatty Acids (MUFA showed that in the Oleic acid was maximum amount of 34% in Spirulina sp. In addition the amount of MUFA in Chlorella sp. was 16.37% of total fatty acids. On the other hand the amount of palmeotic acid in purified culture of Chaetoceros sp. was 30.33% from total content of fatty acids. Analysis of Poly Unsaturated Fatty Acids (PUFA, Linoleic acid (C18:2 (Omega 6, revealed maximum percentage in Spirulina sp. with 18.8%. Results of Alpha linoleic acid (C18:3 (Omega3 analysis showed maximum amount of 9.66% in Chlorella sp. compared to other microalgae with lower omega 3 contents. Spirulina sp. contained maximum amount of Linoleic acid (C18:2 with 18.8% of total fatty acids. Therefore, Spirulina sp. can be considered as a rich source of omega 6 for the purpose of fatty acid extractions. The presence of PUFA in Chlorella sp. and Spirulina sp. was

  10. Antibacterial Actions and Potential Phototoxic Effects of Volatile oils of Foeniculum sp. (fennel, Salvia sp. (sage, Vitis sp. (grape, Lavandula sp. (lavender

    Directory of Open Access Journals (Sweden)

    Elif Ayse Erdogan Eliuz

    2016-09-01

    Full Text Available In the present study, the volatile compounds of essential oil of Foeniculum vulgare (fennel, Salvia officinalis (sage, Vitis vinifera (grape, Lavandula angustifolia (lavender were analysed by gas chromatography-mass spectrometry (GC-MS using the Nist and Willey libraries. It was determined that the main components of Foeniculum sp. were anethole (41.11%, carvacrol (9.18%. whereas main components of Salvia sp were 1.8 cineole (34.09%, caryophyllene (10.95%, camphor (9.44%, α-pinene (8.42%. Vitis sp. contained linoleic acid (36.98%, 2,4-decadienal (30.79%. Finally, volatile component of Lavandula sp. was linalool (33.57%, linalyl acetate (30.74%. Photoxic antibacterial activity of volatile oil of those plants against Escherichia coli (ATCC 25293, Klebsiella pneumoniae (10031, Salmonella thyphimurium, Bacillus subtilis (ATCC 6633, Staphylococcus aureus (ATCC 25925, Enterococcus feacalis (ATCC 29212 were examined by using disc diffusion method. We demonstrated that volatile oil effectively can be activated by a standard LED light. In vitro, significant phototoxicity was demonstrated by volatile oil of Foeniculum sp. and Vitis sp. (P < 0.05, while minor phototoxicity was induced by Lavandula sp. Therefore, volatile oil of plant can be considered as a potential photosensitizer in the photochemical therapy.

  11. Geophysical Measurements in the Beaver Basin, West-Central Utah; Part 1--Slingram, Magnetic, and Self-Potential Profiles

    OpenAIRE

    Flanigan, Vincent J.; Campbell, David L.; U.S. Geological Survey

    1981-01-01

    This report consists of figures showing profile locations (fig. 1, table 1) in the Beaver Basin, west-central Utah, and ground geophysical data collected in September 1980 along these traverses (figs. 2-11). These data consist of slingram electromagnetic (real and imaginary components at 222, 444, 888, 1777, and 3555 Hz), ground magnetic and self-potential measurements collected at 200-foot (61-m) intervals along about 8.8 miles (14.2 km) of survey line. Table 2 lists equipment used. The r...

  12. Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from Halymenia sp. (Rhodophyceae=Análise de dois métodos de secagem sobre o rendimento e atividade de polissacarídeos sulfatados extraídos da rodofícea Halymenia sp.

    Directory of Open Access Journals (Sweden)

    Norma Maria Barros Benevides

    2012-01-01

    Full Text Available Sulfated polysaccharides (SPs have attracted growing interest for various biotechnological applications. We evaluated the efficiency of two methods of drying SPs (M I and II extracted from Halymenia sp Rhodophyceae in order to compare the yield, purification and anticoagulant activity. The total SPs (TSPs were first extracted with papain in 100 mM sodium acetate (pH 5.0 containing cysteine and EDTA (5 mM. The TSPs obtained were dried in an oven (M I or lyophilized (M II and then examined by ion exchange chromatography (DEAE-cellulose using the NaCl gradient technique. The fractions were analyzed by 0.5% agarose gel electrophoresis and the in vitro anticoagulant activity was evaluated by the activated partial thromboplastin time test using normal human plasma and compared to heparin (HEP (193.00 IU mg-1. There was a difference in TSP yield of 19.05% and similar chromatographic SP profiles. Electrophoresis revealed fractions with distinct resolutions. The fractions eluted with 0.75 M of salt (M I and II were the most active, measuring 27.40 and 72.66 IU mg-1, respectively, when compared to HEP. Therefore, obtaining SP with anticoagulant activity from Halymenia sp. is more efficient by freeze-drying.Os polissacarídeos sulfatados (PS têm despertado interesse crescente para diversas aplicações em biotecnologia. Avaliou-se a eficiência de duas metodologias de secagem de PSs (M I e II extraídos da rodofícea Halymenia sp. a fim de se comparar o rendimento, a purificação e a atividade anticoagulante. Inicialmente, os PS totais (PSTs foram extraídos com papaína em tampão acetato de sódio 100 mM (pH 5,0 contendo cisteína e EDTA (5 mM. Os PSTs foram secados em estufa (M I ou liofilizados (M II, seguido por cromatografia de troca iônica (DEAE-celulose utilizando um gradiente de NaCl. As frações foram analisadas por eletroforese em gel de agarose a 0,5% e a atividade anticoagulante “in vitro” avaliada por meio do teste do tempo de

  13. Application of streaming potential method for detection of fractures in granitic rock. part 2; Kamaishi kozan ni okeru ryutai ryudo den`iho tekiyo shiken. 2

    Energy Technology Data Exchange (ETDEWEB)

    Negi, T; Yoneda, Y [Nittetsu Mining Co. Ltd., Tokyo (Japan); Senba, T [Power Reactor and Nuclear Fuel Development Corp., Tokyo (Japan)

    1997-10-22

    Streaming potential method has been applied in the Kamaishi Mine. In FY 1995, self-potential (SP) was monitored on the wall of gallery by varying the pressure in the well with open/close operation of the mouth of well having spring water, which was drilled from the gallery. It was confirmed that SP changes remarkably at the wall having a great number of fractures with spring water. It was considered that the change in SP is due to the streaming potential generated by the flowing underground water from the gallery side to the fractures in the well. In this paper, this continuity of the wall of gallery and the fractures in the well is estimated. In the present tests, SP changes were observed at walls of surrounding galleries by varying the pressure in the well with open/close operation of the mouth of borehole at various drilling depths during drilling of borehole. As a result, SP changes were observed at the wall of gallery when reaching to the depth with increased spring water. The results agreed well with the test results conducted at the same field in FY 1995. It was confirmed that the streaming potential method is a useful method for grasping the hydraulic continuity in the rocks. 2 refs., 9 figs.

  14. Progress in SP-100 tribological coatings

    International Nuclear Information System (INIS)

    Ring, P.J.; Roy, P.; Schuster, G.B.; Busboom, H.J.

    1992-01-01

    The SP-100 reactor will operate at temperatures up to 1500K in high vacuum. To address the SP-100 needs, a tribology development program has been established at GE to investigate candidate coating materials. Materials were selected based on their high thermodynamic stability, high melting point, compatibility with the substrate, and coefficients of thermal expansion similar to niobium-1% zirconium-the candidate structural material for SP-100. An additional requirement was that the deposition processes should be commercially available to coat large components. This paper presents the details regarding the SP-100 Tribology Development Program including background information, specific bearing requirements, basis for coating material selection, testing methods and the initial results covering the early years of this program

  15. Higher derivative analysis for the interpretation of self-potential profiles at southern part of the Nile Delta, Egypt

    International Nuclear Information System (INIS)

    Hafez, M.; EL-Qady, G.; Awad, S.; Elsayed, E.

    2006-01-01

    One of the most important, but also one of the most tedious, problems confronting the mineral industries in dealing with the sulfides and graphite is estimating the depth of a buried object from self-potential anomalies. A peat layer has been encountered in the subsurface Holocene sediments of the eastern part of the Nile Delta at depths range from 7 to 13 meters. The maximum thickness of the peat (3m) has been recorded in the southern part of the Mansoura city and it decreases gradually in a configuration parallel to Damietta branch of the Nile. To elucidate the peat layer in terms of depth and thickness, a self-potential survey was carried at Mansoura city, Nile Delta of Egypt, in terms of eight profiles. Interpretation of data was performed through the correction and processing using the derivative analysis of higher orders by means of an algorithm developed by the authors. Depth, shape factor and the optimum order of the regional components are determined. A rough estimation of the relief of the peat layer was derived. Based in the results, the depth to the peat layer varies from 11.8 up to 13.1 meters which correlates very well with the available information and the lithological data in the area. (author)

  16. Optimization of single plate-serial dilution spotting (SP-SDS) with sample anchoring as an assured method for bacterial and yeast cfu enumeration and single colony isolation from diverse samples.

    Science.gov (United States)

    Thomas, Pious; Sekhar, Aparna C; Upreti, Reshmi; Mujawar, Mohammad M; Pasha, Sadiq S

    2015-12-01

    We propose a simple technique for bacterial and yeast cfu estimations from diverse samples with no prior idea of viable counts, designated as single plate-serial dilution spotting (SP-SDS) with the prime recommendation of sample anchoring (10 0 stocks). For pure cultures, serial dilutions were prepared from 0.1 OD (10 0 ) stock and 20 μl aliquots of six dilutions (10 1 -10 6 ) were applied as 10-15 micro-drops in six sectors over agar-gelled medium in 9-cm plates. For liquid samples 10 0 -10 5 dilutions, and for colloidal suspensions and solid samples (10% w/v), 10 1 -10 6 dilutions were used. Following incubation, at least one dilution level yielded 6-60 cfu per sector comparable to the standard method involving 100 μl samples. Tested on diverse bacteria, composite samples and Saccharomyces cerevisiae , SP-SDS offered wider applicability over alternative methods like drop-plating and track-dilution for cfu estimation, single colony isolation and culture purity testing, particularly suiting low resource settings.

  17. Enhancement of clavulanic acid production by Streptomyces sp MU ...

    African Journals Online (AJOL)

    Purpose: To enhance clavulanic acid production using UV-mutagenesis on Streptomyces sp. NRC77. Methods: UV-mutagenesis was used to study the effect of Streptomyces sp. NRC77 on CA production. Phenotypic and genotypic identification methods of the promising mutant strain were characterized. Optimization of the ...

  18. A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting β-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c.

    Science.gov (United States)

    Wanarska, Marta; Kur, Józef

    2012-08-23

    D-Tagatose is a natural monosaccharide which can be used as a low-calorie sugar substitute in food, beverages and pharmaceutical products. It is also currently being tested as an anti-diabetic and obesity control drug. D-Tagatose is a rare sugar, but it can be manufactured by the chemical or enzymatic isomerization of D-galactose obtained by a β-D-galactosidase-catalyzed hydrolysis of milk sugar lactose and the separation of D-glucose and D-galactose. L-Arabinose isomerases catalyze in vitro the conversion of D-galactose to D-tagatose and are the most promising enzymes for the large-scale production of D-tagatose. In this study, the araA gene from psychrotolerant Antarctic bacterium Arthrobacter sp. 22c was isolated, cloned and expressed in Escherichia coli. The active form of recombinant Arthrobacter sp. 22c L-arabinose isomerase consists of six subunits with a combined molecular weight of approximately 335 kDa. The maximum activity of this enzyme towards D-galactose was determined as occurring at 52°C; however, it exhibited over 60% of maximum activity at 30°C. The recombinant Arthrobacter sp. 22c L-arabinose isomerase was optimally active at a broad pH range of 5 to 9. This enzyme is not dependent on divalent metal ions, since it was only marginally activated by Mg2+, Mn2+ or Ca2+ and slightly inhibited by Co2+ or Ni2+. The bioconversion yield of D-galactose to D-tagatose by the purified L-arabinose isomerase reached 30% after 36 h at 50°C. In this study, a recombinant Pichia pastoris yeast strain secreting β-D-galactosidase Arthrobacter chlorophenolicus was also constructed. During cultivation of this strain in a whey permeate, lactose was hydrolyzed and D-glucose was metabolized, whereas D-galactose was accumulated in the medium. Moreover, cultivation of the P. pastoris strain secreting β-D-galactosidase in a whey permeate supplemented with Arthrobacter sp. 22c L-arabinose isomerase resulted in a 90% yield of lactose hydrolysis, the complete utilization

  19. A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting β-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c

    Directory of Open Access Journals (Sweden)

    Wanarska Marta

    2012-08-01

    Full Text Available Abstract Background D-Tagatose is a natural monosaccharide which can be used as a low-calorie sugar substitute in food, beverages and pharmaceutical products. It is also currently being tested as an anti-diabetic and obesity control drug. D-Tagatose is a rare sugar, but it can be manufactured by the chemical or enzymatic isomerization of D-galactose obtained by a β-D-galactosidase-catalyzed hydrolysis of milk sugar lactose and the separation of D-glucose and D-galactose. L-Arabinose isomerases catalyze in vitro the conversion of D-galactose to D-tagatose and are the most promising enzymes for the large-scale production of D-tagatose. Results In this study, the araA gene from psychrotolerant Antarctic bacterium Arthrobacter sp. 22c was isolated, cloned and expressed in Escherichia coli. The active form of recombinant Arthrobacter sp. 22c L-arabinose isomerase consists of six subunits with a combined molecular weight of approximately 335 kDa. The maximum activity of this enzyme towards D-galactose was determined as occurring at 52°C; however, it exhibited over 60% of maximum activity at 30°C. The recombinant Arthrobacter sp. 22c L-arabinose isomerase was optimally active at a broad pH range of 5 to 9. This enzyme is not dependent on divalent metal ions, since it was only marginally activated by Mg2+, Mn2+ or Ca2+ and slightly inhibited by Co2+ or Ni2+. The bioconversion yield of D-galactose to D-tagatose by the purified L-arabinose isomerase reached 30% after 36 h at 50°C. In this study, a recombinant Pichia pastoris yeast strain secreting β-D-galactosidase Arthrobacter chlorophenolicus was also constructed. During cultivation of this strain in a whey permeate, lactose was hydrolyzed and D-glucose was metabolized, whereas D-galactose was accumulated in the medium. Moreover, cultivation of the P. pastoris strain secreting β-D-galactosidase in a whey permeate supplemented with Arthrobacter sp. 22c L-arabinose isomerase resulted in a 90% yield

  20. Structural control of monogenetic volcanism in the Garrotxa volcanic field (Northeastern Spain) from gravity and self-potential measurements

    Science.gov (United States)

    Barde-Cabusson, S.; Gottsmann, J.; Martí, J.; Bolós, X.; Camacho, A. G.; Geyer, A.; Planagumà, Ll.; Ronchin, E.; Sánchez, A.

    2014-01-01

    We report new geophysical observations on the distribution of subsurface structures associated with monogenetic volcanism in the Garrotxa volcanic field (Northern Spain). As part of the Catalan Volcanic Zone, this Quaternary volcanic field is associated with the European rifts system. It contains the most recent and best preserved volcanic edifices of the Catalan Volcanic Zone with 38 monogenetic volcanoes identified in the Garrotxa Natural Park. We conducted new gravimetric and self-potential surveys to enhance our understanding of the relationship between the local geology and the spatial distribution of the monogenetic volcanoes. The main finding of this study is that the central part of the volcanic field is dominated by a broad negative Bouguer anomaly of around -0.5 mGal, within which a series of gravity minima are found with amplitudes of up to -2.3 mGal. Inverse modelling of the Bouguer data suggests that surficial low-density material dominates the volcanic field, most likely associated with effusive and explosive surface deposits. In contrast, an arcuate cluster of gravity minima to the NW of the Croscat volcano, the youngest volcano of this zone, is modelled by vertically extended low-density bodies, which we interpret as a complex ensemble of fault damage zones and the roots of young scoria cones. A ground-water infiltration zone identified by a self-potential anomaly is associated with a steep horizontal Bouguer gravity gradient and interpreted as a fault zone and/or magmatic fissure, which fed the most recent volcanic activity in the Garrotxa. Gravimetric and self-potential data are well correlated and indicate a control on the locations of scoria cones by NNE-SSW and NNW-SSE striking tectonic features, which intersect the main structural boundaries of the study area to the north and south. Our interpretation of the data is that faults facilitated magma ascent to the surface. Our findings have major implications for understanding the relationship

  1. PRODUCTION OF FIBRINOLYTIC ENZYME (NATTOKINASE) FROM BACILLUS SP.

    OpenAIRE

    Padma Singh, Rekha Negi*, Vani Sharma, Alka Rani, Pallavi and Richa Prasad

    2018-01-01

    During present study Nattokinase which is a novel fibrinolytic enzyme was produced by Bacillus sp. To screen and extract nattokinase enzyme from Bacillus sp. were isolated from soil of different agricultural field by serial dilution method. Out of 10 isolate, one strain i.e. B3 produced nattokinase on screening medium. B3 was identified by biochemical characterization. The caseinolytic activity of Nattokinase was 0.526 U/ml and the selected isolate Bacillus sp. could produce active nattokinas...

  2. Forward Modeling and validation of a new formulation to compute self-potential signals associated with ground water flow

    Directory of Open Access Journals (Sweden)

    A. Bolève

    2007-10-01

    Full Text Available The classical formulation of the coupled hydroelectrical flow in porous media is based on a linear formulation of two coupled constitutive equations for the electrical current density and the seepage velocity of the water phase and obeying Onsager's reciprocity. This formulation shows that the streaming current density is controlled by the gradient of the fluid pressure of the water phase and a streaming current coupling coefficient that depends on the so-called zeta potential. Recently a new formulation has been introduced in which the streaming current density is directly connected to the seepage velocity of the water phase and to the excess of electrical charge per unit pore volume in the porous material. The advantages of this formulation are numerous. First this new formulation is more intuitive not only in terms of establishing a constitutive equation for the generalized Ohm's law but also in specifying boundary conditions for the influence of the flow field upon the streaming potential. With the new formulation, the streaming potential coupling coefficient shows a decrease of its magnitude with permeability in agreement with published results. The new formulation has been extended in the inertial laminar flow regime and to unsaturated conditions with applications to the vadose zone. This formulation is suitable to model self-potential signals in the field. We investigate infiltration of water from an agricultural ditch, vertical infiltration of water into a sinkhole, and preferential horizontal flow of ground water in a paleochannel. For the three cases reported in the present study, a good match is obtained between finite element simulations performed and field observations. Thus, this formulation could be useful for the inverse mapping of the geometry of groundwater flow from self-potential field measurements.

  3. Analisis Fosfor pada Cacing Tanah (Megascolex sp. dan Fridericia sp.) Secara Spektrofotometri Sinar Tampak

    OpenAIRE

    Safira, Cut Shafa

    2015-01-01

    Earthworm is natural resource which can be used for medication due to its highly amount of minerals. One of these minerals is phosphorus. The aim of this research are to identify, determine and know the difference content of phosphorus in Megascolex sp. and Fridericia sp. Qualitative analysis shows positive results with addition of ammonium molybdate 4% and BaCl2 5%. Quantitative analysis was done using visible spectrophotometer with ascorbic acid method, measuring blu-colored molybdenum ...

  4. Methods of statistical analysis, dimensioning and determination of production of electric power for description of the scheme applied to wind in Botucatu/ SP region, Brazil; Metodos de analise estatistica, dimensionamento e determinacao da energia eletrica produtivel para descricao do regime eolico aplicados na regiao de Botocatu-SP

    Energy Technology Data Exchange (ETDEWEB)

    Gabriel Filho, Luis Roberto Almeida [Universidade Estadual Paulista Julio de Mesquita Filho (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas], email: gabrielfilho@tupa.unesp.br; Cremasco, Camila P. [Faculdade de Tecnologia (FATEC), Presidente Prudente, SP (Brazil); Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil). Dept. de Bioestatistica; Verri, Juliano A. [Universidade Estadual Paulista Julio de Mesquita Filho (FCT/UNESP), Presidente Prudente, SP (Brazil). Dept. de Matematica, Estatistica e Computacao; Viais Neto, Daniel dos S. [Faculdade de Tecnologia (FATEC), Presidente Prudente, SP (Brazil); Universidade Estadual Paulista Julio de Mesquita Filho (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas; Seraphim, Odivaldo J. [Universidade Estadual Paulista Julio de Mesquita Filho (FCA/UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas. Dept. de Engenharia Rural

    2011-07-01

    The wind energy is an abundant source of renewable energy, clean and available almost everywhere. For its use, studies are needed to adequately describe its intensity for statistical methods and design for wind turbines. The objective is to structure the mathematical methods used to conduct a general description of the wind, to establish a description of the wind regime using the parameters described using the Weibull function and the analytical model of power from a wind turbine, as well as applications of these methods show. This work was developed at Rural Empowerment Lab of FCA/UNESP in Botucatu-SP. For the application of methods were utilized measurements of wind speed and direction, between 2004 and 2005, obtained by anemometer RM Young Wind Monitor-Campbell. As a result, they estimated the wind behavior and distribution of wind in the region, with 78 kWh of energy production, relatively low potential to supply a small house. Moreover, this energy could possibly be applied in ambient illumination, power supply for electric fences and irrigation of vegetables. (author)

  5. SP-100 Program overview

    International Nuclear Information System (INIS)

    Truscello, V.C.

    1984-01-01

    The SP-100 Program is expected to go through three phases: technology assessment and advancement, ground testing, and flight qualification. Currently the program is in the two- to three-year technology assessment and advancement stage. Goals are to identify the space nuclear power system concept that best meets anticipated requirements of future space missions, assess the technical feasibility of that concept, and establish a cost and schedule for developing the concept. The SP-100 Project Office has begun the implementation activities needed to meet these goals. With regard to refractory alloys, a better data base will be required before we move ahead in the program from technology assessment to ground demonstration

  6. Development of Self-Potential Tomography for Early Warning System of rainfall induced Landslides: Electro-kinetic Effects and Sandbox Experiments

    Science.gov (United States)

    Hattori, K.; Yamazaki, T.; Terajima, T.; Huang, Q.

    2017-12-01

    Electro-kinetic effects is one of the possible mechanism for ULF electromagnetic phenomena preceeding landlisdes and large earthquakes. To understand general tendencies of electromagnetic changes related to electro-kinetic effects, we struggle with the integrated research to clarify the coupling among hydrological, geotechnical, and electromagnetic changes. Our final goal is to understand the ULF elecromagnetic phenomena in order to develop a simple technology for earthquake monitoring/forecasting. So, in this paper, we first show the observed waveforms possibly related to the Boso slow slip events in 2003 and 2007 and indoor landslide experiments with artificial rainfall. Then, numerical computations on the self-potential variation by the simulated groundwater flow, and compare the results with those observed by laboratory experiments. In the result, the simulated self-potential variation is consistent with observed one. FInally, we developed self-potential tomography to estimate the ground water condition. And we also characterize the pressure from the self-potential data, and compare the result with observed pressure head that is measured by pore-pressure gauge and found that the inverted pressure head is consistent with observed one. In addition, we apply the self-potential data observed by the flume test. The estimated pressure head from observed self-potential data shows the consistency with observed pressure head. And estimated pressure head also show the characteristic distribution before the landslide occurred. These facts are highly suggestive in effectiveness of the self-potential tomography to monitor groundwater changes associated with landslide. The details will be given in our presentation.

  7. Green Decision Making: Sustainable Transport and Systemic Planning (SP)

    DEFF Research Database (Denmark)

    Leleur, Steen

    and infrastructure assessment and on the methodology and process of systemic planning (SP). SP theory development has interchanged with practical application and testing of the SP approach in a large number of cases. The word systemic in SP indicates that complex planning problems and provision of decision support...... in today’s strategic planning needs a focus on what may be addressed as systemic insights in balance with more conventional, systematically-based findings where causal linkages can be modelled and made use of. In practice this means that SP is based on a study-specific combination of hard (quantitative......) and soft (qualitative) operations research (OR) methods; especially the latter have a function as regards knowledge generation that relates to obtaining systemic insights. Furthermore, SP applies a process that drives group-based learning forward. The group should be formed with the different stakeholder...

  8. In Vitro Antimicrobial Potential of the Lichen Parmotrema sp. Extracts against Various Pathogens.

    Science.gov (United States)

    Chauhan, Ritika; Abraham, Jayanthi

    2013-07-01

    The ongoing increasing antibiotic resistance is one of the biggest challenges faced by global public health. The perennial need for new antimicrobials against a background of increasing antibiotic resistance in pathogenic and opportunistic microorganisms obliges the scientific community to constantly develop new drugs and antimicrobial agents. Lichens are known prolific sources of natural antimicrobial drugs and biologically active natural products. This study was aimed to explore in vitro antimicrobial activity of lichen Parmotrema sp. The methanol and aqueous extracts of lichen Parmotrema sp. was extracted using Soxhlet extractor. Antibiotic assessment of methanol and aqueous extracts was done against eight bacterial (Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Salmonella sp., Shigella sp., Enterococci faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniae,) clinical pathogens and five plant pathogenic fungal strains (Aspergillus terreus strain JAS1, Scedosporium sp. JAS1, Ganoderma sp. JAS4, Candida tropicalis and Fusarium sp.) by Kirby-Bauer method. The methanol lichen Parmotrema sp. extract inhibited all the test organisms. The highest antibacterial activity was found against Pseudomonas aeruginosa and Staphylococcus aureus. The weakest activity was manifested in Salmonella sp. and Scedosporium sp. JAS1. Strong antifungal effect was found against Ganoderma sp. JAS4 and Fusarium sp. The aqueous lichen Parmotrema sp. extract revealed neither antibacterial nor antifungal activity. The present study shows that tested lichen Parmotrema sp. extracts demonstrated a strong antimicrobial effect. That suggests the active components from methanol extracts of the investigated lichen Parmotrema sp. can be used as natural antimicrobial agent against pathogens.

  9. Cryptosporidium sp. in lizards

    Czech Academy of Sciences Publication Activity Database

    Koudela, Břetislav; Modrý, D.

    1998-01-01

    Roč. 45, č. 1 (1998), s. 8 ISSN 1066-5234. [Cryptosporidium sp. in lazards. 01.01.1998-02.01.1998, Praha] R&D Projects: GA ČR GA508/95/0273; GA AV ČR IPP2020702 Subject RIV: fp - Other Medical Disciplines

  10. Quality improvement on half-fin anchovy (Setipinna taty) fish sauce by Psychrobacter sp. SP-1 fermentation.

    Science.gov (United States)

    Zheng, Bin; Liu, Yu; He, Xiaoxia; Hu, Shiwei; Li, Shijie; Chen, Meiling; Jiang, Wei

    2017-10-01

    A method of improving fish sauce quality during fermentation was investigated. Psychrobacter sp. SP-1, a halophilic protease-producing bacterium, was isolated from fish sauce with flavor-enhancing properties and non-biogenic amine-producing activity. The performance of Psychrobacter sp. SP-1 in Setipinna taty fish sauce fermentation was investigated further. The inoculation of Psychrobacter sp. SP-1 did not significantly affect pH or NaCl concentration changes (P > 0.05), although it significantly increased total moderately halophilic microbial count, protease activity, total soluble nitrogen content and amino acid nitrogen content, and also promoted the umami taste and meaty aroma (P sauce quality by fermentation. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  11. Detection of Salmonella sp. from porcine origin: a comparison between a PCR method and standard microbiological techniques Detecção de Salmonella sp. em amostras de origem suína: comparação entre a técnica da Reação em Cadeia da Polimerase e o isolamento bacteriano convencional

    Directory of Open Access Journals (Sweden)

    Sandra Maria Ferraz Castagna

    2005-12-01

    Full Text Available The aim of this study was to compare a polymerase chain reaction (PCR method combined with selective enrichment in Rappaport-Vassiliadis broth (PCR-RVB with standard microbiological techniques (SMT for the generic detection of Salmonella in samples of porcine origin. Two hundred sixty eight field samples consisting of 42 sets of pooled porcine mandibular lymph nodes and tonsils, 44 samples of intestinal content, 38 pork sausage meat samples and 144 samples of feed collected from swine farms were submitted to the PCR-RVB and SMT protocols. Salmonella was detected in 54 samples using the PCR-RVB assay and in 42 samples by SMT, three of the SMT Salmonella-positive samples (one each of S. Derby, S. Panama and S. Typhimurium being Salmonella-negative by PCR-RVB. For the PCR-RVB method 15 Salmonella-positive samples were negative by SMT, a significant difference according to the Mac Nemar's chi-squared test (p=0.0153. Subsequent serological typing of the SMT isolates showed the following Salmonella serovars, the number of positive samples being given in parentheses: Typhimurium (12; Bredeney (10; Panama (5; Saint-paul (5; Minnesota (3; Mbandaka (2; Derby (1; Enteritidis (1; Orion (1 and Salmonella sp. (2. We concluded that, although the use of both PCR-RVB and SMT increased the number of positive samples, the PCR-RVB, due to its higher sensitivity and greater speed in giving results, can be implemented to detect Salmonella in samples of porcine origin.O objetivo desse estudo foi comparar um método de Reação em Cadeia da Polimerase (PCR combinado com enriquecimento seletivo em caldo Rappaport-Vassiliadis (PCR-RVB com as técnicas de isolamento bacteriano convencional (SMT para a detecção do gênero Salmonella em amostras de origem suína. Duzentas e sessenta e oito amostras de campo, compostas por: 42 "pools" de linfonodos mandibulares e tonsilas, 44 amostras de conteúdo intestinal, 38 amostras de massa de embutidos e 144 amostras de ra

  12. Evaluation of Etest and macrodilution broth method for antifungal susceptibility testing of Candida sp strains isolated from oral cavities of AIDS patients

    Directory of Open Access Journals (Sweden)

    SILVA Maria do Rosário R.

    2002-01-01

    Full Text Available A comparison of the Etest and the reference broth macrodilution susceptibility test for fluconazole, ketoconazole, itraconazole and amphotericin B was performed with 59 of Candida species isolated from the oral cavities of AIDS patients. The Etest method was performed according to the manufacturer's instructions, and the reference method was performed according to National Committee for Clinical Laboratory Standards document M27-A guidelines. Our data showed that there was a good correlation between the MICs obtained by the Etest and broth dilution methods. When only the MIC results at ± 2 dilutions for both methods were considered, the agreement rates were 90.4% for itraconazole, ketoconazole and amphotericin B and 84.6% for fluconazole of the C. albicans tested. In contrast, to the reference method, the Etest method classified as susceptible three fluconazole-resistant isolates and one itraconazole-resistant isolate, representing four very major errors. These results indicate that Etest could be considered useful for antifungal sensitivity evaluation of yeasts in clinical laboratories.

  13. The geothermal system of Caviahue-Copahue Volcanic Complex (Chile-Argentina): New insights from self-potential, soil CO2 degassing, temperature measurements and helium isotopes, with structural and fluid circulation implications.

    Science.gov (United States)

    Roulleau, Emilie; Bravo, Francisco; Barde-Cabusson, Stephanie; Pizarro, Marcela; Muños, Carlos; Sanchez, Juan; Tardani, Daniele; Sano, Yuji; Takahata, Naoto; de Cal, Federico; Esteban, Carlos

    2016-04-01

    Geothermal systems represent natural heat transfer engines in a confined volume of rock which are strongly influenced by the regional volcano-tectonic setting controlling the formation of shallow magmatic reservoirs, and by the local faults/fracture network, that permits the development of hydrothermal circulation cells and promote the vertical migration of fluids and heat. In the Southern Volcanic Zone of Chile-Argentina, geothermal resources occur in close spatial relationship with active volcanism along the Cordillera which is primarily controlled by the 1000 km long, NNE Liquiñe-Ofqui Fault Zone (LOFZ), an intra-arc dextral strike-slip fault system, associated with second-order intra-arc anisotropy of overall NE-SW (extensional) and NW-SE orientation (compressional). However there is still a lack of information on how fault network (NE and WNW strinking faults) and lithology control the fluid circulation. In this study, we propose new data of dense self-potential (SP), soil CO2 emanation and temperature (T) measurements within the geothermal area from Caviahue-Copahue Volcanic Complex (CCVC), coupled with helium isotopes ratios measured in fumaroles and thermal springs. We observe that inside the geothermal system the NE-striking faults, characterized by a combination of SP-CO2 and T maxima with high 3He/4He ratios (7.86Ra), promote the formation of high vertical permeability pathways for fluid circulation. Whereas, the WNW-striking faults represent low permeability pathways for hydrothermal fluids ascent associated with moderate 3He/4He ratios (5.34Ra), promoting the infiltration of meteoric water at shallow depth. These active zones are interspersed by SP-CO2- T minima, which represent self-sealed zones (e.g. impermeable altered rocks) at depth, creating a barrier inhibiting fluids rise. The NE-striking faults seem to be associated with the upflow zones of the geothermal system, where the boiling process produces a high vapor-dominated zone close to the

  14. Structural controls on fluid circulation at the Caviahue-Copahue Volcanic Complex (CCVC) geothermal area (Chile-Argentina), revealed by soil CO2 and temperature, self-potential, and helium isotopes

    Science.gov (United States)

    Roulleau, Emilie; Bravo, Francisco; Pinti, Daniele L.; Barde-Cabusson, Stéphanie; Pizarro, Marcela; Tardani, Daniele; Muñoz, Carlos; Sanchez, Juan; Sano, Yuji; Takahata, Naoto; de la Cal, Federico; Esteban, Carlos; Morata, Diego

    2017-07-01

    Natural geothermal systems are limited areas characterized by anomalously high heat flow caused by recent tectonic or magmatic activity. The heat source at depth is the result of the emplacement of magma bodies, controlled by the regional volcano-tectonic setting. In contrast, at a local scale a well-developed fault-fracture network favors the development of hydrothermal cells, and promotes the vertical advection of fluids and heat. The Southern Volcanic Zone (SVZ), straddling Chile and Argentina, has an important, yet unexplored and undeveloped geothermal potential. Studies on the lithological and tectonic controls of the hydrothermal circulation are therefore important for a correct assessment of the geothermal potential of the region. Here, new and dense self-potential (SP), soil CO2 and temperature (T) measurements, and helium isotope data measured in fumaroles and thermal springs from the geothermal area located in the north-eastern flank of the Copahue volcanic edifice, within the Caviahue Caldera (the Caviahue-Copahue Volcanic Complex - CCVC) are presented. Our results allowed to the constraint of the structural origin of the active thermal areas and the understanding of the evolution of the geothermal system. NE-striking faults in the area, characterized by a combination of SP, CO2, and T maxima and high 3He/4He ratios (up to 8.16 ± 0.21Ra, whereas atmospheric Ra is 1.382 × 10- 6), promote the formation of vertical permeability preferential pathways for fluid circulation. WNW-striking faults represent low-permeability pathways for hydrothermal fluid ascent, but promote infiltration of meteoric water at shallow depths, which dilute the hydrothermal input. The region is scattered with SP, CO2, and T minima, representing self-sealed zones characterized by impermeable altered rocks at depth, which create local barriers for fluid ascent. The NE-striking faults seem to be associated with the upflowing zones of the geothermal system, where the boiling process

  15. Effect of garlic solution to Bacillus sp. removal

    Science.gov (United States)

    Zainol, N.; Rahim, S. R.

    2018-04-01

    Biofilm is a microbial derived sessile community characterized by cells that are irreversibly attached to a substratum or interface to each other, embedded in a matrix of extracellular polymeric substances that they have produced. Bacillus sp. was used as biofilm model in this study. The purpose of this study is to determine the effect of Garlic solution in term of ratio of water and Garlic solution (W/G) and ratio of Garlic solution to Bacillus sp. (GS/B) on Bacillus sp removal. Garlic solution was used to remove Bacillus sp. In this study, Garlic solution was prepared by crushing the garlic and mixed it with water. the Garlic solution was added into Bacillus sp. mixture and mixed well. The mixture then was spread on nutrient agar. The Bacillus sp. weight on agar plate was measured by using dry weight measurement method. In this study, initially Garlic solution volume and Garlic solution concentration were studied using one factor at time (OFAT). Later two-level-factorial analysis was done to determine the most contributing factor in Bacillus sp. removal. Design Expert software (Version 7) was used to construct experimental table where all the factors were randomized. Bacilus sp removal was ranging between 42.13% to 99.6%. The analysis of the results showed that at W/G of 1:1, Bacillus sp. removal increased when more Garlic solution was added to Bacillus sp. Effect of Garlic solution to Bacillus sp. will be understood which in turn may be beneficial for the industrial purpose.

  16. Control of Branchionus sp. and Amoeba sp. in cultures of Arthrospira sp. Control de Branchionus sp. y Amoeba sp. en cultivos de Arthrospira sp.

    Directory of Open Access Journals (Sweden)

    Carlos Méndez

    2012-09-01

    Full Text Available Cultivation of cyanobacterium Arthrospira sp. has been developed in many countries for the production of proteins, pigments and other compounds. Outdoor mass cultures are often affected by biological contamination, drastically reducing productivity as far as bringing death. This study evaluates the control of Branchionus sp. and Amoeba sp. with two chemical compounds: urea (U and ammonium bicarbonate (AB, in laboratory conditions and outdoor mass culture of Arthrospira sp. The lethal concentration 100 (LC100 at 24 h for Branchionus sp. and Amoeba sp. determined was of 60-80 mg L-1 (U and 100-150 mg L-1 (AB. The average effective inhibition concentration for 50% of the population (IC50 in Arthrospira sp., after 72 h, was 80 mg L-1 (U and 150 mg L-1 (AB. The application of doses of 60 mg L-1 (U or 100 mg L-1 (AB in the outdoor mass culture of this contaminated microalga, completely inhibited grazing and did not affect the growth of Arthrospira sp. but rather promoted rapid recovery of algal density at levels prior to infestation. These compounds provided an economical and effective control of predators in cultures of Arthrospira sp.El cultivo de la cianobacteria Arthrospira sp. ha sido desarrollado en muchos países para la obtención de proteínas, pigmentos y otros compuestos. Cultivo que a nivel industrial se ve afectado frecuentemente por contaminación biológica, reduciendo drásticamente la productividad hasta causar la muerte. Este estudio evalúa el control de Branchionus sp. y de Amoeba sp. con dos compuestos químicos, la urea (U y bicarbonato de amonio (AB en cultivos de Arthrospira sp. La concentración letal 100 (LC100 determinada a las 24 h para Branchionus sp. y Amoeba sp. fue de 60-80 mg L-1 (U y 100-150 mg L-1 (AB. La concentración media de inhibición efectiva, después de 72 h, para el 50% de la población (IC50 en Arthrospira fue de 80 mg L-1 (U y 150 mg L-1 (AB. La aplicación de dosis de 60 mg L-1 (U ó 100 mg L-1 (AB en

  17. Volvatellin, caulerpenyne-related product fromt he sacoglossan Volvatella sp.

    Digital Repository Service at National Institute of Oceanography (India)

    Fontana, A; Ciavatta, M.L; Mollo, E.; Naik, C.G.; Wahidullah, S.; DeSouza, L; Cimino, G.

    Volvatellin (4) is a highly unstable terpene isolated from the extracts of the Indian opisthobranch mollusk Volvatella sp. The structure and the relative stereochemistry of 4 were determined by NMR methods. The paper also describes a hypothetical...

  18. A new double digestion ligation mediated suppression PCR method for simultaneous bacteria DNA-typing and confirmation of species: an Acinetobacter sp. model.

    Directory of Open Access Journals (Sweden)

    Karolina Stojowska

    Full Text Available We have designed a new ddLMS PCR (double digestion Ligation Mediated Suppression PCR method based on restriction site polymorphism upstream from the specific target sequence for the simultaneous identification and differentiation of bacterial strains. The ddLMS PCR combines a simple PCR used for species or genus identification and the LM PCR strategy for strain differentiation. The bacterial identification is confirmed in the form of the PCR product(s, while the length of the PCR product makes it possible to differentiate between bacterial strains. If there is a single copy of the target sequence within genomic DNA, one specific PCR product is created (simplex ddLMS PCR, whereas for multiple copies of the gene the fingerprinting patterns can be obtained (multiplex ddLMS PCR. The described ddLMS PCR method is designed for rapid and specific strain differentiation in medical and microbiological studies. In comparison to other LM PCR it has substantial advantages: enables specific species' DNA-typing without the need for pure bacterial culture selection, is not sensitive to contamination with other cells or genomic DNA, and gives univocal "band-based" results, which are easy to interpret. The utility of ddLMS PCR was shown for Acinetobacter calcoaceticus-baumannii (Acb complex, the genetically closely related and phenotypically similar species and also important nosocomial pathogens, for which currently, there are no recommended methods for screening, typing and identification. In this article two models are proposed: 3' recA-ddLMS PCR-MaeII/RsaI for Acb complex interspecific typing and 5' rrn-ddLMS PCR-HindIII/ApaI for Acinetobacter baumannii intraspecific typing. ddLMS PCR allows not only for DNA-typing but also for confirmation of species in one reaction. Also, practical guidelines for designing a diagnostic test based on ddLMS PCR for genotyping different species of bacteria are provided.

  19. A new double digestion ligation mediated suppression PCR method for simultaneous bacteria DNA-typing and confirmation of species: an Acinetobacter sp. model.

    Science.gov (United States)

    Stojowska, Karolina; Krawczyk, Beata

    2014-01-01

    We have designed a new ddLMS PCR (double digestion Ligation Mediated Suppression PCR) method based on restriction site polymorphism upstream from the specific target sequence for the simultaneous identification and differentiation of bacterial strains. The ddLMS PCR combines a simple PCR used for species or genus identification and the LM PCR strategy for strain differentiation. The bacterial identification is confirmed in the form of the PCR product(s), while the length of the PCR product makes it possible to differentiate between bacterial strains. If there is a single copy of the target sequence within genomic DNA, one specific PCR product is created (simplex ddLMS PCR), whereas for multiple copies of the gene the fingerprinting patterns can be obtained (multiplex ddLMS PCR). The described ddLMS PCR method is designed for rapid and specific strain differentiation in medical and microbiological studies. In comparison to other LM PCR it has substantial advantages: enables specific species' DNA-typing without the need for pure bacterial culture selection, is not sensitive to contamination with other cells or genomic DNA, and gives univocal "band-based" results, which are easy to interpret. The utility of ddLMS PCR was shown for Acinetobacter calcoaceticus-baumannii (Acb) complex, the genetically closely related and phenotypically similar species and also important nosocomial pathogens, for which currently, there are no recommended methods for screening, typing and identification. In this article two models are proposed: 3' recA-ddLMS PCR-MaeII/RsaI for Acb complex interspecific typing and 5' rrn-ddLMS PCR-HindIII/ApaI for Acinetobacter baumannii intraspecific typing. ddLMS PCR allows not only for DNA-typing but also for confirmation of species in one reaction. Also, practical guidelines for designing a diagnostic test based on ddLMS PCR for genotyping different species of bacteria are provided.

  20. A comparison of analytical methods for detection of [14C]trichloro acetic acid-derived radioactivity in needles and branches of spruce (Picea sp.)

    International Nuclear Information System (INIS)

    Kretzschmar, M.; Matucha, M.; Uhlirova, H.

    1994-01-01

    The branches (wood and needles) of spruces of varying age treated with [ 14 C]trichloro acetic acid (3.7 GBq/mmol) were studied, using the following methods: Qualitative: - Conventional macroautoradiography with X-ray film and histological classification. Quantitative: - 14 C combustion analysis with the sample oxidizer A 307 (Canberra/Packard) followed by measurement of radioactivity using the LS counter 6000 (Beckman Instrumentts); - digital autoradiography with the Digital Autoradiograph LB 286 (Berthold GmbH); -digital autoradiography with the Bio-imaging Analyzer BAS 2000 (Fuji Film Co.). (orig.)

  1. A sp2+sp3 hybridized carbon allotrope transformed from AB stacking graphyne and THD-graphene

    Directory of Open Access Journals (Sweden)

    Yu Zhang

    2018-01-01

    Full Text Available New carbon allotropes can be designed by combining sp, sp2 and sp3 three hybridization states. And the hybridization states or coordination numbers of carbon atoms can be changed by applying high pressure on carbon materials. In this study, a common high pressure phase (named as TBBC transformed from AB-stacking graphyne or THD-graphene is predicted. Its kinetic stability is examined using finite displacement method. We find that the sp2 and sp3 hybridized carbon atoms behave different vibration features at high frequency region. Both graphene-like and diamond-like vibration peaks occurs. Phase transition energy barriers from both graphyne and THD-graphene to TBBC are estimated. Electronic structure calculations show that the TBBC is an indirect semiconductor with a bandgap of 0.66 eV. The ideal tensile strength of TBBC is high in [0001] and [11¯00] directions, but is weak along [12¯10] direction.

  2. Truncated recombinant human SP-D attenuates emphysema and type II cell changes in SP-D deficient mice

    Directory of Open Access Journals (Sweden)

    Mühlfeld Christian

    2007-10-01

    Full Text Available Abstract Background Surfactant protein D (SP-D deficient mice develop emphysema-like pathology associated with focal accumulations of foamy alveolar macrophages, an excess of surfactant phospholipids in the alveolar space and both hypertrophy and hyperplasia of alveolar type II cells. These findings are associated with a chronic inflammatory state. Treatment of SP-D deficient mice with a truncated recombinant fragment of human SP-D (rfhSP-D has been shown to decrease the lipidosis and alveolar macrophage accumulation as well as production of proinflammatory chemokines. The aim of this study was to investigate if rfhSP-D treatment reduces the structural abnormalities in parenchymal architecture and type II cells characteristic of SP-D deficiency. Methods SP-D knock-out mice, aged 3 weeks, 6 weeks and 9 weeks were treated with rfhSP-D for 9, 6 and 3 weeks, respectively. All mice were sacrificed at age 12 weeks and compared to both PBS treated SP-D deficient and wild-type groups. Lung structure was quantified by design-based stereology at the light and electron microscopic level. Emphasis was put on quantification of emphysema, type II cell changes and intracellular surfactant. Data were analysed with two sided non-parametric Mann-Whitney U-test. Main Results After 3 weeks of treatment, alveolar number was higher and mean alveolar size was smaller compared to saline-treated SP-D knock-out controls. There was no significant difference concerning these indices of pulmonary emphysema within rfhSP-D treated groups. Type II cell number and size were smaller as a consequence of treatment. The total volume of lamellar bodies per type II cell and per lung was smaller after 6 weeks of treatment. Conclusion Treatment of SP-D deficient mice with rfhSP-D leads to a reduction in the degree of emphysema and a correction of type II cell hyperplasia and hypertrophy. This supports the concept that rfhSP-D might become a therapeutic option in diseases that are

  3. Detection of Brucella sp. infection through serological, microbiological, and molecular methods applied to buffaloes in Maranhão State, Brazil.

    Science.gov (United States)

    Dos Santos, Larissa Sarmento; Sá, Joicy Cortez; Dos Santos Ribeiro, Diego Luiz; Chaves, Nancyleni Pinto; da Silva Mol, Juliana Pinto; Santos, Renato Lima; da Paixão, Tatiane Alves; de Carvalho Neta, Alcina Vieira

    2017-04-01

    The aim of the current study is to diagnose Brucella spp. infection using methods such as serology, bacterial isolation, and molecular analysis in buffaloes bred in Maranhão State. In order to do so, 390 samples of buffalo serum were subjected to serological tests, to Rose Bengal Plate Test (RBPT) and to 2-mercaptoethanol (2-ME) combined with slow agglutination test (SAT). Vaginal swabs were collected from seropositive animals and subjected to bacterial isolation and to generic PCR. According to the serological test, 16 animals had a positive reaction to the confirmatory test (2-ME/SAT). As for bacterial isolation, three samples resulted in the isolation of Brucella spp.-characteristic colonies, which were confirmed through PCR. These results confirmed Brucella spp. infection in the buffalo herd from Maranhão State.

  4. SP mountain data analysis

    Science.gov (United States)

    Rawson, R. F.; Hamilton, R. E.; Liskow, C. L.; Dias, A. R.; Jackson, P. L.

    1981-01-01

    An analysis of synthetic aperture radar data of SP Mountain was undertaken to demonstrate the use of digital image processing techniques to aid in geologic interpretation of SAR data. These data were collected with the ERIM X- and L-band airborne SAR using like- and cross-polarizations. The resulting signal films were used to produce computer compatible tapes, from which four-channel imagery was generated. Slant range-to-ground range and range-azimuth-scale corrections were made in order to facilitate image registration; intensity corrections were also made. Manual interpretation of the imagery showed that L-band represented the geology of the area better than X-band. Several differences between the various images were also noted. Further digital analysis of the corrected data was done for enhancement purposes. This analysis included application of an MSS differencing routine and development of a routine for removal of relief displacement. It was found that accurate registration of the SAR channels is critical to the effectiveness of the differencing routine. Use of the relief displacement algorithm on the SP Mountain data demonstrated the feasibility of the technique.

  5. Improved detection of Candida sp. fks hot spot mutants by using the method of the CLSI M27-A3 document with the addition of bovine serum albumin.

    Science.gov (United States)

    Garcia-Effron, Guillermo; Park, Steven; Perlin, David S

    2011-05-01

    Echinocandins are highly bound to serum proteins, altering their antifungal properties. The addition of 50% human serum to the MIC assay improves the identification of echinocandin-resistant Candida spp. harboring fks hot spot mutations. However, this modification cannot readily be applied to the method of the CLSI M27-A3 document due to safety and standardization difficulties. The aim of this study was to evaluate commercial bovine serum albumin (BSA) as a safe and standardized alternative to human serum. A collection of 28 echinocandin-susceptible strains, 10 Candida parapsilosis sensu lato strains (with naturally reduced echinocandin susceptibility), and 40 FKS hot spot mutants was used in this work. When RPMI 1640 was used for susceptibility testing, wild-type strains and fks mutants showed MIC range overlaps (-2, -1, and -3 2-fold-dilution steps separated these populations for anidulafungin, caspofungin, and micafungin, respectively). On the other hand, the addition of BSA to RPMI 1640 differentially increased echinocandin MIC values for these groups of strains, allowing better separation between populations, with no MIC range overlaps for any of the echinocandin drugs tested. Moreover, the use of RPMI-BSA reduced the number of fks hot spot mutant isolates for which MIC values were less than or equal to the upper limit for the wild type (very major errors) from 9, 2, and 7 with RPMI alone to 3, 0, and 3 for anidulafungin, caspofungin, and micafungin, respectively. When RPMI-BSA was used to study the susceptibility of C. parapsilosis sensu lato species to echinocandins, the strains behaved as anidulafungin- and micafungin-resistant isolates (MIC, ≥8 μg/ml). These data support the need for a revision of the CLSI protocol for in vitro testing of echinocandin susceptibility in order to identify all or most of the fks hot spot mutants. Also, caspofungin could be used as a surrogate marker of reduced susceptibility to echinocandins.

  6. Post-neonatal drop in alveolar SP-A expression

    DEFF Research Database (Denmark)

    Stray-Pedersen, Arne; Vege, Ashild; Stray-Pedersen, Asbjorg

    2008-01-01

    BACKGROUND: Surfactant protein A (SP-A) is synthesized in the lung and is a part of the innate immune system. The aim of this study was to evaluate the expression of SP-A in lung tissue from fetuses, infants, children and adults with special regard to sudden infant death syndrome (SIDS). METHODS......: A total of 160 cases were studied; 19 fetuses and neonates, 59 SIDS and 49 explained infant deaths below 1 year of age, 19 toddlers and 14 adults. Immunohistochemical detection of SP-A using monoclonal antibodies was performed by microscopy of lung tissue specimens collected at autopsy. A scoring system...

  7. SP-100 Test Site

    International Nuclear Information System (INIS)

    Cox, C.M.; Mahaffey, M.K.; Miller, W.C.

    1988-01-01

    Preparatory activities are well under way at Hanford to convert the 309 Containment Building and its associated service wing to a 2.5 MWt nuclear test facility for the SP-100 Ground Engineering System (GES) test. Preliminary design is complete, encompassing facility modifications, a secondary heat transport system, a large vacuum system to enclose the high temperature reactor, a test assembly cell and handling system, control and data processing systems, and safety and auxiliary systems. The design makes extensive use of existing equipment to minimize technical risk and cost. Refurbishment of this equipment is 75% complete. The facility has been cleared of obstructing equipment from its earlier reactor test. Current activities are focusing on definitive design and preparation of the Preliminary Safety Analysis Report (PSAR) aimed at procurement and construction approvals and schedules to achieve reactor criticality by January 1992. 6 refs

  8. Scalability of Parallel Spatial Direct Numerical Simulations on Intel Hypercube and IBM SP1 and SP2

    Science.gov (United States)

    Joslin, Ronald D.; Hanebutte, Ulf R.; Zubair, Mohammad

    1995-01-01

    The implementation and performance of a parallel spatial direct numerical simulation (PSDNS) approach on the Intel iPSC/860 hypercube and IBM SP1 and SP2 parallel computers is documented. Spatially evolving disturbances associated with the laminar-to-turbulent transition in boundary-layer flows are computed with the PSDNS code. The feasibility of using the PSDNS to perform transition studies on these computers is examined. The results indicate that PSDNS approach can effectively be parallelized on a distributed-memory parallel machine by remapping the distributed data structure during the course of the calculation. Scalability information is provided to estimate computational costs to match the actual costs relative to changes in the number of grid points. By increasing the number of processors, slower than linear speedups are achieved with optimized (machine-dependent library) routines. This slower than linear speedup results because the computational cost is dominated by FFT routine, which yields less than ideal speedups. By using appropriate compile options and optimized library routines on the SP1, the serial code achieves 52-56 M ops on a single node of the SP1 (45 percent of theoretical peak performance). The actual performance of the PSDNS code on the SP1 is evaluated with a "real world" simulation that consists of 1.7 million grid points. One time step of this simulation is calculated on eight nodes of the SP1 in the same time as required by a Cray Y/MP supercomputer. For the same simulation, 32-nodes of the SP1 and SP2 are required to reach the performance of a Cray C-90. A 32 node SP1 (SP2) configuration is 2.9 (4.6) times faster than a Cray Y/MP for this simulation, while the hypercube is roughly 2 times slower than the Y/MP for this application. KEY WORDS: Spatial direct numerical simulations; incompressible viscous flows; spectral methods; finite differences; parallel computing.

  9. Electrical imaging and self-potential survayes to study the geological setting of the Quaternary, slope depositsin the Agri high valley (Southern Italy)

    OpenAIRE

    M. Schiattarella; S. Piscitelli; V. Lapenna; S. I. Giano

    2000-01-01

    We present the results of a geophysical survey carried out to outline the structural modelling of Quaternary slopedeposits in the northern part of the Agri high valley (Basilicata, Southern Italy). Quaternary folding and brittle deformations of the subaerial slope deposits have been studied combining electrical imaging and self-potential surveys with geological structural analysis. This integrated approach indicates that the area underwent both transpressional and transtensional tectonics dur...

  10. Self-potential monitoring around wells in Mutnovsky geothermal field, Kamchatka; Kamchatka hanto mutnovsky deno chinetsui shuhen no shizen den`i monitoring

    Energy Technology Data Exchange (ETDEWEB)

    Matsushima, N.; Tosha, T.; Ishito, K. [Geological Survey of Japan Ibaragi (Japan); Delemen, I.; Kiryukhin, A. [Institute of Volcanology Far East Branch Russia Academy of Sciences (Russia)

    1997-07-01

    Mutnovsky is a geothermal field which lies to the south of and about 80km away from Petropavlovsk, Kamchatsky, the state capital of Kamchatka. The geothermal survey has been conducted since 1978 in this field. In this study, the self-potential variation was observed by monitoring the potential difference between places near and far from a well in the same region. Then, the self-potential associated with spurting vapor from a well was analyzed using a model of the self-potential generated from the steaming current coupled with the flow of hot water in the porous medium. As results of an experiment on the spurt of stream, vapor containing 80% stream in weight was exhausted at a mass flow rate of 30kg/sec at 100degC from wells. Since the specific enthalpy of this vapor is 2225kJ/kg, the underground geothermal storage layer was estimated to be a state of liquid and vapor two-phase. 9 refs., 6 figs.

  11. Sandbox experiments on Uraninite Ore: ERT and SP measurments.

    Science.gov (United States)

    Singh, R. K.

    2015-12-01

    Nuclear energy, considering its own intrinsic merits, would be a leading source for meeting the energy requirement in present and future scenario. Concealed Uranium deposits under sedimentary cover, with poor surface indications calls for reorientation of survey with large inputs involving integrated geophysical approach. Sand Box experiments have been carried out over Uraninite ore. The tank is a glass fish tank (height 39 cm, length 75 cm, width 30 cm). It was filled with sand up to 35 cm high. The sand was saturated from below to minimize the entrapment of the gas bubbles. The average size for sand grains is ~ 0.295mm. The formation factor of the sand is 3.5, with a negligible surface conductivity because of the coarse nature of the sand grains. The dimension of considered Uraninite ore sample is 4cm x 4cm x 4cm. The depth of top of the ore sample is kept at 3cm. In this paper both resistivity and self-potential measurements were carried out for possible detection of Uraninite. The resistivity measurements were made with 64 non-polarizable electrodes using Electrical Resistivity Tomography (ERT) equipment of FlashRes Universal developed by ZZ Resistivity Imaging Pty. Ltd. We have used screws of length 3cm as electrodes. The separation between these electrodes are ~ 1cm. The resistivity tomography results clearly outlines the target Uraninite body. The resistivity tomography results also detects small heterogeneities associated with air bubbles possibly due to unsaturated pore spaces. SP measurements were made using two non-polarizing Pb/PbCl2 electrodes and a Fluke 289 voltmeter (sensitivity 0.001 mV, internal impedance 100 MOhm). The reference electrode was located on the corner of the sandbox. The other electrode was used to scan the electrical potential at the surface of the sand. SP measurements were made with a spacing of 3 cm over the same ERT profile. The SP results also shows a dip (or a low SP anomaly) over the target ore body sample. Thus, both SP and

  12. Impact of serum SP-A and SP-D levels on comparison and prognosis of idiopathic pulmonary fibrosis

    OpenAIRE

    Wang, Kai; Ju, Qing; Cao, Jing; Tang, Wenze; Zhang, Jian

    2017-01-01

    Abstract Background and objective: Idiopathic pulmonary fibrosis (IPF) has a poor prognosis in general; however, it is heterogeneous to detect relative biomarkers for predicting the disease progression. Serum biomarkers can be conveniently collected to detect and help to differentially diagnose IPF and predict IPF prognosis. This meta-analysis aimed to evaluate the use of serum surfactant proteins A and D (SP-A and SP-D) for differential diagnosis and prognosis of IPF. Methods: Relevant artic...

  13. From spent graphite to amorphous sp2+sp3 carbon-coated sp2 graphite for high-performance lithium ion batteries

    Science.gov (United States)

    Ma, Zhen; Zhuang, Yuchan; Deng, Yaoming; Song, Xiaona; Zuo, Xiaoxi; Xiao, Xin; Nan, Junmin

    2018-02-01

    Today, with the massive application of lithium ion batteries (LIBs) in the portable devices and electric vehicles, to supply the active materials with high-performances and then to recycle their wastes are two core issues for the development of LIBs. In this paper, the spent graphite (SG) in LIBs is used as raw materials to fabricate two comparative high-capacity graphite anode materials. Based on a microsurgery-like physical reconstruction, the reconstructed graphite (RG) with a sp2+sp3 carbon surface is prepared through a microwave exfoliation and subsequent spray drying process. In contrast, the neural-network-like amorphous sp2+sp3 carbon-coated graphite (AC@G) is synthesized using a self-reconfigurable chemical reaction strategy. Compared with SG and commercial graphite (CG), both RG and AC@G have enhanced specific capacities, from 311.2 mAh g-1 and 360.7 mAh g-1 to 409.7 mAh g-1 and 420.0 mAh g-1, at 0.1C after 100 cycles. In addition, they exhibit comparable cycling stability, rate capability, and voltage plateau with CG. Because the synthesis of RG and AC@G represents two typical physical and chemical methods for the recycling of SG, these results on the sp2+sp3 carbon layer coating bulk graphite also reveal an approach for the preparation of high-performance graphite anode materials derived from SG.

  14. Geophysical monitoring of the EDZ during a gallery excavation in the Opalinus clay of the Mont Terri URL: anomalies of noble gases and self-potential associated with fractures and fluid dynamics in a horizontal borehole

    International Nuclear Information System (INIS)

    Maineult, A.; Mahiouz, K.; Lesparre, N.; Thomas, B.; Lavielle, B.; Nussbaum, C.; Wieczorek, K.; Gibert, D.; Kergosien, B.; Nicollin, F.

    2012-01-01

    Document available in extended abstract form only. The research underground rock laboratory (URL) of Mont Terri (Switzerland) was established in 1996 in a Mesozoic clay-stone formation (Opalinus Clay). It is aimed at studying the hydro-mechanical, thermal, geochemical and geophysical behaviour of argillaceous formations in the context of radioactive waste disposal. The EZ-G experiments were designed to monitor the EDZ evolution. The EZ-G08 experiment started in September 2007 to study the EDZ changes at different time scales during the tunnelling of gallery Ga08 starting from the northern part of the URL toward the end-face of the gallery Ga04. Before the excavation process started, we characterized the petrology and the structural properties of the core of the horizontal, 12-m long borehole BEZ-G5 drilled in the end-face of gallery Ga04 (first 2 meters in the shaly facies, the rest in the sandy facies). We quantified its noble gas content for studying gas transport processes in rocks and connected fracture networks. Depletion in He can be observed in the EDZ and other noble gases can also increase as desaturation processes occur. Inflows of water occurred in the borehole few weeks after its drilling until the junction of galleries Ga08 and Ga04. Water amounts of few litres were commonly released in other boreholes in the URL. We recorded the natural electrical potentials (self-potentials - SP), in BEZ-G5. SP originate from the movement of fluid, the diffusion of concentration or temperature gradients, and are sensitive to any change occurring in them. Borehole BEZ-G5 was equipped with a specific device, on which custom-made electrodes were fixed every 15 cm. The signals showed coherent perturbations during the drilling operations in the boreholes BEZ-G12 and in the end-face of the gallery Ga04. Afterwards, an early, strong but rather smooth increase of a few tens of mV, followed by a very slow decrease of much smallest amplitude, can be observed in some signals

  15. Biodegradation of resorcinol byPseudomonas sp.

    Institute of Scientific and Technical Information of China (English)

    Nader Hajizadeh; Najibeh Shirzad; Ali Farzi; Mojtaba Salouti; Azra Momeni

    2016-01-01

    ABSTRACT Objective:To investigate the ability ofPseudomonas sp. isolated from East Azarbaijan, Iran in bioremediation of resorcinol. Methods: Resorcinol biodegradation was evaluated using spectrophotometry and confirmed by gas chromatography-mass spectroscopy. Results:This isolate was able to remove up to 37.12% of resorcinol from contaminated water. Reusability experiments had confirmed the biodegradation process which produced seven intermediate compounds. These intermediates were characterized by gas chromatography-mass spectroscopy technique. The products of resorcinol biodegradation were apparently 1, 4-cyclohexadiene, nonadecene, 2-heptadecanone, 1-isopropyl-2-methoxy-4-methylbenzene, hexadecanoic acid, 9-octadecenoic acid, phenol and 5-methyl-2-(1-methylethyl). Conclusions: The findings revealed thatPseudomonas sp. is able to degrade resorcinol. Because of being an indigenous organism, this isolate is more compatible with the climate of the northwest region of Iran and possibly will be used for degradation of other similar aromatic compounds.

  16. Bradysia sp. em morangueiro Bradysia sp. in strawberry

    Directory of Open Access Journals (Sweden)

    Bernadete Radin

    2009-04-01

    Full Text Available No trabalho, relatam-se os primeiros registros de Bradysia sp. (Insecta: Diptera: Sciaridae em morangueiro (Fragaria x ananassa Duch., cultivado no Município de Eldorado do Sul, RS. O cultivo foi realizado em sacolas com três metros de comprimento, preenchidas com substrato composto de casca de arroz e turfa, dispostas horizontalmente sobre bancadas de madeira, em ambiente protegido. A presença de Bradysia sp. foi observada na segunda quinzena de agosto de 2005. Neste trabalho, estão descritos os sintomas apresentados no morangueiro pela praga, prováveis conseqüências sobre o aparecimento de doenças e uma breve descrição morfológica da Bradysia sp., adulto e fase larval.This paper describes the first record of Bradysia sp. (Insecta; Diptera; Sciaridae in strawberry (Fragaria x ananassa, cultivated in the city of Eldorado do Sul, RS, Brazil. Strawberry was planted in plastic bags filled with a mixture of burnt rice hulls and peat and cultivated in a greenhouse. The presence of Bradysia sp was noticed in the second fortnight of August, 2005. The symptoms in strawberry and the probable consequences in terms of disease arising were described in the present study, as well as the morphological characterization of Bradysia sp. and its illustrations.

  17. Yersinia pekkanenii sp. nov.

    Science.gov (United States)

    Murros-Kontiainen, Anna; Johansson, Per; Niskanen, Taina; Fredriksson-Ahomaa, Maria; Korkeala, Hannu; Björkroth, Johanna

    2011-10-01

    The taxonomic position of three strains from water, soil and lettuce samples was studied by using a polyphasic taxonomic approach. The strains were reported to lack the virulence-encoding genes inv and virF in a previous study. Controversially, API 20 E and some other phenotypic tests suggested that the strains belong to Yersinia pseudotuberculosis, which prompted this polyphasic taxonomic study. In both the phylogenetic analyses of four housekeeping genes (glnA, gyrB, recA and HSP60) and numerical analyses of HindIII and EcoRI ribopatterns, the strains formed a separate group within the genus Yersinia. Analysis of the 16S rRNA gene sequences showed that the strains were related to Yersinia aldovae and Yersinia mollaretii, but DNA-DNA hybridization analysis differentiated them from these species. Based on the results of the phylogenetic and DNA-DNA hybridization analyses, a novel species, Yersinia pekkanenii sp. nov., is proposed. The type strain is ÅYV7.1KOH2(T) ( = DSM 22769(T)  = LMG 25369(T)).

  18. Antimicrobial peptides from Capsicum sp.

    African Journals Online (AJOL)

    ajl yemi

    2011-12-30

    Dec 30, 2011 ... Key words: Antimicrobial peptides, Capsicum sp, Capsicum chinense, chili pepper, agronomical options, ..... of this human activity is resumed by the simple phrase: produce .... It will be interesting to scale the AMPs extraction.

  19. Pengaruh Perbedaan Konsentrasi Ekstrak Sargassum sp. dan Lama Penyimpanan terhadap Oksidasi Lemak pada Fillet Ikan Patin (Pangasius sp.

    Directory of Open Access Journals (Sweden)

    Fatin Hidayati

    2017-05-01

    storage time in inhibiting the oxidation process in catfish fillet. The materials used in this research were Sargassum sp. extracts and catfish fillet. This research was using experimental laboratories research method with factorial Completely Randomized Research Design (CRD of 2 factors which were concentrations of Sargassum sp. extracts (0%, 1%, 1.5%, and 2% and storage time (0 day, 2 days, 4 days, and 6 days. The results showed that the adding of different concentrations of Sargassum sp. extracts and storage time gave significant effect to the PV value, TBA value, fat content, protein content, moisture content, as well as organoleptic point (P < 0.05. The results obtained from stage I research were the yields of Sargassum sp. with ethanol 96% solvent was 1.39%, phenol content was 1.813%, flavonoid was 0.278%, and antioxidant activity was 99.1659 ppm (categorized as strong antioxidant. The results obtained from stage II research were the PV value were ranged from 2.03 to 19.82 meq/kg, TBA value were ranged from 0.63 to 6.72 mg.mal/kg. The 1.5% concentration was the best concentration of Sargassum sp. extracts in inhibiting the lipid oxidation of catfish fillet during storage. Keywords: Antioxidant, Sargassum sp. Extracts, Storage Time, Lipid Oxidation, Catfish Fillet Citation: Hidayati, F., Darmanto, Y.S. dan Romadhon. (2017. Pengaruh Perbedaan Konsentrasi Ekstrak Sargassum sp. dan Lama Penyimpanan terhadap Oksidasi Lemak pada Fillet Ikan Patin (Pangasius sp.. Jurnal Ilmu Lingkungan, 15(1, 64-73, doi:10.14710/jil.15.1.64-73

  20. Screening of biodiesel production from waste tuna oil (Thunnus sp.), seaweed Kappaphycus alvarezii and Gracilaria sp.

    Science.gov (United States)

    Alamsjah, Mochammad Amin; Abdillah, Annur Ahadi; Mustikawati, Hutami; Atari, Suci Dwi Purnawa

    2017-09-01

    Biodiesel has several advantages over solar. Compared to solar, biodiesel has more eco-friendly characteristic and produces lower greenhouse gas emissions. Biodiesel that is made from animal fats can be produced from fish oil, while other alternative sources from vegetable oils are seaweed Kappaphycus alvarezii and Gracilaria sp. Waste tuna oil (Thunnus sp.) in Indonesia is commonly a side product of tuna canning industries known as tuna precook oil; on the other hand, seaweed Gracilaria sp. and Kappaphycus alvarezii are commonly found in Indonesia's seas. Seaweed waste that was used in the present study was 100 kg and in wet condition, and the waste oil was 10 liter. The seaweed was extracted with soxhletation method that used n-hexane as the solvent. To produce biodiesel, trans esterification was performed on the seaweed oil that was obtained from the soxhletation process and waste tuna oil. Biodiesel manufactured from seaweed K. alvarezii obtained the best score in flash point, freezing point, and viscosity test. However, according to level of manufacturing efficiency, biodiesel from waste tuna oil is more efficient and relatively easier compared to biodiesel from waste K. alvarezii and Gracilaria sp.

  1. Change in surface SP caused by pressure buildup observed at the Nigorikawa geothermal area; Nigorikawa chiiki ni okeru atsuryoku buildup ji no shizen den`i henka

    Energy Technology Data Exchange (ETDEWEB)

    Yasukawa, K; Yano, Y; Matsushima, N; Ishido, T [Geological Survey of Japan, Tsukuba (Japan); Takahashi, M; Suzuki, I; Aoyama, K; Kuwano, T

    1996-10-01

    To examine the effect of change of subsurface flow system on the surface SP (self potential), SP measurements were carried out before and after the pressure buildup and drawdown during the periodic inspection at Nigorikawa area. Relation between the SP distribution and the observed data was also examined by 2-D numerical simulation. Tendency was found that the SP increased gradually with the production near the production well, decreased during the pressure buildup, and increased again during the drawdown. There were some points having the reverse tendency in the surrounding area. Behavior during the pressure buildup and drawdown was not clear. The resistivity near the ground surface was low ranging between 2 and 5 ohm/m within the Nigorikawa basin. The variation of SP was not so large when compared with the measuring error. The SP profiles on the secondary section passing in the center of caldera at the production stop and at one week after the production start were well corresponded with the profiles under natural conditions which were reproduces using the 2-D model. It was considered that the SP profile before the production stop was affected by the production. 12 refs., 6 figs., 1 tab.

  2. Electrical imaging and self-potential surveys to study the geological setting of the quaternary slope deposits in the Agri high valley (Southern Italy)

    Energy Technology Data Exchange (ETDEWEB)

    Giano, S I; Schiattarella, M [Basilicata Univ., Potenza (Italy). Centro di Geodinamica; Lapenna, V; Piscitelli, S [Consiglio Nazionale delle Ricerche, Tito, PZ (Italy). Ist. di Metodologie Avanzate di Analisi Ambientale

    2000-04-01

    The paper presents the results of a geophysical survey carried out to outline the structural modelling of quarternary slope deposits in the northern part of Agri high valley (Basilicata region, Italy). Quaternary folding and brittle deformations of the subaerial slope deposits have been studied combining electrical imaging and self-potential surveys with geological structural analysis. This integrated approach indicates that the area underwent both transpressional and transtensional tectonics during Pleistocene times as testified by the existence of a push up structure in the basement buried by deformed Quaternary breccias. On this basis, the valley appears to be a more complex structure than a simple extensional graben, as traditionally assumed in the literature.

  3. Microbial transformation of citral by Penicillium sp..

    Science.gov (United States)

    Esmaeili, Akbar; Tavassoli, Afsaneh

    2010-01-01

    Thymol is present in the essential oils from herbs and spices, such as thyme. It is produced by these plant species as a chemical defense against phytopathogenic microorganisms. Therefore, this compound has attracted great attention in food industry, i.e., it has been used as a natural preservative in foods such as cheese to prevent fungal growth. Previous studies concerning the biotransformation of nerol by Penicillium sp. and microbial transformation of citral by sporulated surface cultures method (SSCM) of Penicillium digitatum have been reported. The objective of this research was to study the pathway involved during biotransformation of citral by Penicillium sp. using two methods. The culture preparation was done using different microbial methods and incubation periods to obtain Penicillium for citral biotransformation. The biotransformation products were identified by gas chromatography (GC) and gas chromatography/mass spectroscopy (GC/MS). A comparison of the two methods showed that SSCM was more effective, its major products were thymol (21.5 %), geranial (18.6 %) and nerol (13.7 %). LM produced only one compound — thymol — with a low efficiency.

  4. Botanical pesticides effect from shells of bean’s cashew nut on biological agents of trichoderma sp. and gliocladium sp.

    Science.gov (United States)

    Bande, L. O. S.; Mariadi; Gusnawaty, HS; Nuriadi; Trisulpa, L.; Rahmania

    2018-02-01

    A shell of cashew nut (Anacardium occidentanle) has contained Cashew Nut Shell Liquid (CNSL) that is used as botanical pesticides. CNSL oil consists of active substance such as anacardat acid, cardol and cardanol. Utilization of the pesticides from shells of cashew nut to control pests and diseases of plants would be affected on biological agents. The objective of this research was to investigate pesticides inhibition on the increase of mycelium Trichoderma sp. and Gliocladium sp. by in vitro method. The tested concentration sample consisted of 0.0% (control), 2.5%, 7.5% and 10.0% in PDA media. The results of this research showed that 2.5% botanical pesticides concentration could minimize mycelium of Trichoderma sp. and Gliocladium sp. 22.73% and 21.04% respectively and also the increase shells of cashew extract could be affected the increase of mycelium inhibition. The extract with 2.5% concentration was the recommended concentration to control of fruit rot diseases and if concentration was 10.0% then its inhibition become 54.98% and 49.35%, respectively. The results proved that uncontrolled utilization of the pesticides could be affected on decrease of Trichoderma sp. and Gliocladium sp. growth.

  5. Impact of serum SP-A and SP-D levels on comparison and prognosis of idiopathic pulmonary fibrosis

    Science.gov (United States)

    Wang, Kai; Ju, Qing; Cao, Jing; Tang, Wenze; Zhang, Jian

    2017-01-01

    Abstract Background and objective: Idiopathic pulmonary fibrosis (IPF) has a poor prognosis in general; however, it is heterogeneous to detect relative biomarkers for predicting the disease progression. Serum biomarkers can be conveniently collected to detect and help to differentially diagnose IPF and predict IPF prognosis. This meta-analysis aimed to evaluate the use of serum surfactant proteins A and D (SP-A and SP-D) for differential diagnosis and prognosis of IPF. Methods: Relevant articles were searched in PubMed, Embase, and Chinese National Knowledge Infrastructure databases and reviewed by 2 independent readers. Standard mean difference (SMD) and 95% confidence interval (CI) were calculated to assess the difference in serum levels of SP-A/D among patients with IPF, when compared to patients with non-IPF interstitial lung disease (ILD), pulmonary infection, and healthy control. Hazard ratio (HR) and 95% CI were used to compare the relative risk of mortality. Results: Twenty-one articles (totalling 1289 IPF patients) were included in final meta-analysis. Serum SP-A levels were significantly higher in patients with IPF than in patients with non-IPF ILD (SMD: 1.108 [0.584, 1.632], P infection (SMD: 1.320 [0.999, 1.640], P SMD: 2.802 [1.901, 3.702], P SMD: 0.459 [−0.000, 0.919], P = .050). Serum SP-D levels were significantly higher in patients with IPF than in patients with pulmonary infection (SMD: 1.308 [0.813, 1.803], P SMD: 2.235 [1.739, 2.731], P < .001). Risk of death in patients with IPF and elevated serum SP-A was increased 39% compared to patients with low SP-A groups. Elevated SP-D increased risk by 111% when compared to low SP-D. In acute exacerbation of IPF, serum SP-A/D were higher than those in stable stage. The comparisons and prognosis might be different in Asian and Caucasian patients. Conclusions: Serum SP-A/D detection might be useful for differential diagnosis and prediction of survival in patients with IPF. PMID:28591049

  6. SP-100 reactor cell activation

    International Nuclear Information System (INIS)

    Wilcox, A.D.

    1991-09-01

    There are plans to test the SP-100 space reactor for 2 yr in the test facility shown in Figure 1. The vacuum vessel will be in the reactor experiment (RX) cell surrounded by an inert gas atmosphere. It is proposed that the reactor test cell could contain removable-water- shielding tanks to reduce the residual activation dose rates in the test cell after the tests are completed. This reduction will allow the facility to be considered for other uses after the SP-100 tests are completed. The radiation dose rates in the test cell were calculated for several configurations of water-shielding tanks to help evaluate this concept

  7. Potential topical natural repellent against Ae. aegypti, Culex sp. and Anopheles sp. mosquitoes

    Directory of Open Access Journals (Sweden)

    Dewi Nur Hodijah

    2014-08-01

    Full Text Available AbstrakLatar belakang:Minyak atsiri daun sirih diketahui mempunyai daya proteksi. Dibuatkan losion berdasarkan pengantar sediaan farmasi yang ditambahkan minyak atsiri daun nilam. Sediaan losion dipilih agar dapat menempel lebih lama di permukaan kulit. Tujuan penelitian ini untuk membandingkan daya proteksi antara losion dengan penambahan minyak nilam dan losion tanpa penambahan minyak nilam dibandingkan daya proteksi dengan DEET. Metode: Penelitian ini merupakan penelitian eksperimental laboratorium. Semua nyamuk uji berasal dari insektarium laboratorium penelitian kesehatan Loka litbang P2B2 Ciamis. Konsentrasi minyak atsiri daun sirih dalam losion adalah 4%; konsentrasi minyak nilam sebagai zat pengikat adalah 0,4%. Formula yang digunakan yaitu formula dasar yang ada pada pengantar sediaan farmasi. Uji repelensi dilakukan dengan menggunakan metoda yang direkomendasikan oleh Komisi pestisida.Hasil: Dihasilkan formulasi losion yang stabil dan masih memenuhi standar formulasi sediaan. Berdasarkan hasil, diperoleh data bahwa DEET dan losion hasil modifikasi memiliki rata-rata daya proteksi di atas 90% selama 6 jam terhadap nyamuk Ae.aegypti dan Culex sp. Kesimpulan: Penambahan minyak nilam pada losion sirih dapat meningkatkan daya proteksi terhadap hinggapan nyamuk Ae. aegypti dan Culex sp. (Health Science Indones 2014;1:44-8Kata kunci:repelen alamiah, minyak atsiri, daun sirih, daun nilam, Ae. aegypti, Culex sp.AbstractBackground: Betel leaf essential oil lotion has been known to have insect repellent properties. A lotion was made based on a pharmaceutical formula from a monograph where patchouli leaf essential oil was added. A lotion preparation was intended to enhance adherence of the formula on the surface of the skin. The purpose of this study was to compare protection percentage of lotion with patchouli oil and without patchouli oil lotion compared to DEET.Methods: This study is an experimental laboratory-based research. All mosquitoes

  8. Electromagnetic methods to characterize the Savoia di Lucania waste dump (Southern Italy)

    Science.gov (United States)

    Bavusi, M.; Rizzo, E.; Lapenna, V.

    2006-11-01

    The aim of this work is the joint application and integration of non-invasive geoelectrical methods for studying the landfill of Savoia di Lucania (Southern Italy). This landfill for its engineering features and small dimensions (70 m × 30 m × 6 m) represents an optimal test site to assess a geophysical survey protocol for municipal solid waste landfills investigation and monitoring. The landfill of Savoia di Lucania has been built with a reinforced concrete material and coated with a high-density polyethylene (HDPE) liner. Three electrical resistivity tomographies (ERT), two self-potential (SP) map surveys and one induced polarization (IP) section have been performed, both in the surrounding area and inside the waste landfill. The geophysical investigations have well defined some buried boundaries of the landfill basin and localized the leachate accumulation zones inside the dumpsite. Comparison of our results with other engineering and geological investigations could be the key for evaluating the integrity of the HDPE liner. Finally, the joint use of the ERT, IP and SP methods seems to be a promising tool for studying and designing new monitoring systems able to perform a time-lapse analysis of waste landfill geometry and integrity.

  9. ESTIMASI ALIRAN AIR LINDI TPA BANTAR GEBANG BEKASI MENGGUNAKAN METODA SP

    Directory of Open Access Journals (Sweden)

    Syamsu Rosid

    2012-02-01

    Full Text Available Air lindi merupakan limbah sampah organik yang biasanya diproduksi dari sampah rumah tangga. Pencemaran air tanah (groundwater oleh air lindi menjadi ancaman yang serius bagi masyarakat. Penduduk Bantar Gebang, Bekasi merasa terancam kehidupannya dengan adanya tempat pembuangan akhir (TPA sampah yang dikirim dari DKI Jakarta. Beberapa penduduk di sekitar TPA mengeluhkan bahwa air sumurnya agak bau dan tidak lagi terasa segar. Untuk mengetahui seperti apa polusi air lindi yang terjadi, ke arah mana dan sudah sejauh mana sebarannya, dan memetakan daerah resiko tinggi terkena polusi maka telah dilakukan pengukuran geolistrik metoda self potential (SP di sebelah Tenggara dan Selatan daerah TPA Bantar Gebang, Bekasi. Dari data SP diketahui bahwa aliran air tanah bawah permukaan di daerah tersebut berarah Selatan ke Utara. Meskipun lokasi TPA berada di Utara daerah penelitian, limbah air lindi diduga telah mencemari air tanah bawah permukaan hingga radius ratusan meter dari lokasi TPA. Penyebaran air limbah ini  diperkirakan melalui proses osmosis, mekanisme kapilaritas dan proses elektrokinetik.   Kata kunci: air lindi, metoda SP, TPA Bantar Gebang.

  10. Blastocystis sp. and other intestinal parasites in hemodialysis patients.

    Science.gov (United States)

    Kulik, Rose Anne; Falavigna, Dina Lúcia Morais; Nishi, Letícia; Araujo, Silvana Marques

    2008-08-01

    Chronic renal insufficiency disease (CRI) leads to uremia in hemodialysis patients and induces a state of immunodepression that results in higher frequencies of infections and diarrhea. Hemodialysis patients resident in the city of Campo Mourão, Paraná, Brazil were analyzed from April 2006 through September 2007 for Blastocystis sp. and other intestinal parasites and for associated diarrhea. Fecal samples from 86 hemodialysis patients and 146 healthy (reference) persons were examined by standard methods for detecting ova, larvae and cysts, which included preservation in 10% formalin and the Kinyoun method. Thirty-three hemodialysis patients (45.1%) and 36 reference individuals (25.7%) were found to be parasitized. The differences in the percentages of parasitism and polyparasitism between the reference group and the chronic renal patients was significant (p= 0.0318 and 0.0019, respectively). Blastocystis sp. (18%-20.1%), Endolimax nana (14%-16.3%), Cryptosporidium sp. (4%-4.7%) and Entamoeba coli (4%-4.7%) were the most frequent protozoa found in the hemodialysis patients. Parasitism was not significantly associated with diarrhea (p=0.9947) or with decreased white blood cell counts (p=0.7046) in these individuals. Because parasitic infections may be an important comorbidity factor in hemodialysis patients, we suggest that parasitological stool examinations, especially for Blastocystis sp. and Cryptosporidium sp., be included in routine medical follow-up examinations of these patients.

  11. Blastocystis sp. and other intestinal parasites in hemodialysis patients

    Directory of Open Access Journals (Sweden)

    Rose Anne Kulik

    Full Text Available Chronic renal insufficiency disease (CRI leads to uremia in hemodialysis patients and induces a state of immunodepression that results in higher frequencies of infections and diarrhea. Hemodialysis patients resident in the city of Campo Mourão, Paraná, Brazil were analyzed from April 2006 through September 2007 for Blastocystis sp. and other intestinal parasites and for associated diarrhea. Fecal samples from 86 hemodialysis patients and 146 healthy (reference persons were examined by standard methods for detecting ova, larvae and cysts, which included preservation in 10% formalin and the Kinyoun method. Thirty-three hemodialysis patients (45.1% and 36 reference individuals (25.7% were found to be parasitized. The differences in the percentages of parasitism and polyparasitism between the reference group and the chronic renal patients was significant (p= 0.0318 and 0.0019, respectively. Blastocystis sp. (18%-20.1%, Endolimax nana (14%-16.3%, Cryptosporidium sp. (4%-4.7% and Entamoeba coli (4%-4.7% were the most frequent protozoa found in the hemodialysis patients. Parasitism was not significantly associated with diarrhea (p=0.9947 or with decreased white blood cell counts (p=0.7046 in these individuals. Because parasitic infections may be an important comorbidity factor in hemodialysis patients, we suggest that parasitological stool examinations, especially for Blastocystis sp. and Cryptosporidium sp., be included in routine medical follow-up examinations of these patients.

  12. Production of *sp67*Ga at the Oslo Cyclotron

    International Nuclear Information System (INIS)

    Bjoernstad, T.; Holtebekk, T.

    1983-01-01

    A method for production of *sp67*Ga at the Oslo Cyclotron is described. The method is based on the nuclear reaction *sp68*Zn (p,2n)*sp67*Ga. The target is natural zinc metal of thickness 1.3 mm fixed by a thin alloy layer to a copper disc for efficient cooling during irradiation. By applying a beam of 29 MeV protons, a maximum production yield of approx. 1.8 mCi/*my*Ah was obtained. By demanding a contamination level of *sp66*Ga <=1%, the ''useful'' yield after a decaytime of 88 h is approx. 0.8 mCi/*my*Ah. Gallium has been separated carrierfree from the zinc matrix by cation exchange from 7.5M hydrocloric acid solutions and prepared as citrate complex at pH 5.5. After sterile filtering, autoclavation, pyrogene testing and analysis for iron and zinc, the *sp67*Ga-radiopharmaceutical has been applied in human investigations at the Ullevaal hospital in Oslo. (Auth.)

  13. On Embedding N2R Structures in Optical Fiber OMS-SP Ring

    DEFF Research Database (Denmark)

    Riaz, Muhammad Tahir; Pedersen, Jens Myrup; Nielsen, Rasmus Hjorth

    2006-01-01

    The objective of this paper is to propose methods for embedding N2R structures in optical fiber OMS-SP rings. The OMS-SP ring supports full mesh structure and restoration on the optical level. The N2R structures have been proven to be superior to other degree 3 network structures. Two main mapping...

  14. Cognitive-Behavioral Therapy for Suicide Prevention (CBT-SP): Treatment Model, Feasibility, and Acceptability

    Science.gov (United States)

    Stanley, Barbara; Brown, Gregory; Brent, David A.; Wells, Karen; Poling, Kim; Curry, John; Kennard, Betsy D.; Wagner, Ann; Cwik, Mary F.; Klomek, Anat Brunstein; Goldstein, Tina; Vitiello, Benedetto; Barnett, Shannon; Daniel, Stephanie; Hughes, Jennifer

    2009-01-01

    Objective: To describe the elements of a manual-based cognitive-behavioral therapy for suicide prevention (CBT-SP) and to report its feasibility in preventing the recurrence of suicidal behavior in adolescents who have recently attempted suicide. Method: The CBT-SP was developed using a risk reduction and relapse prevention approach and…

  15. Electrical imaging and self-potential survayes to study the geological setting of the Quaternary, slope depositsin the Agri high valley (Southern Italy

    Directory of Open Access Journals (Sweden)

    M. Schiattarella

    2000-06-01

    Full Text Available We present the results of a geophysical survey carried out to outline the structural modelling of Quaternary slopedeposits in the northern part of the Agri high valley (Basilicata, Southern Italy. Quaternary folding and brittle deformations of the subaerial slope deposits have been studied combining electrical imaging and self-potential surveys with geological structural analysis. This integrated approach indicates that the area underwent both transpressional and transtensional tectonics during Pleistocene times as testified by the existence of a push up structure in the basement buried by deformed Quaternary breccias. On this basis, the valley appears to be a more complex structure than a simple extensional graben, as traditionally assumed in the literature.

  16. Results of an interlaboratory method performance study for the size determination and quantification of silver nanoparticles in chicken meat by single-particle inductively coupled plasma mass spectrometry (sp-ICP-MS).

    Science.gov (United States)

    Weigel, Stefan; Peters, Ruud; Loeschner, Katrin; Grombe, Ringo; Linsinger, Thomas P J

    2017-08-01

    Single-particle inductively coupled plasma mass spectrometry (sp-ICP-MS) promises fast and selective determination of nanoparticle size and number concentrations. While several studies on practical applications have been published, data on formal, especially interlaboratory validation of sp-ICP-MS, is sparse. An international interlaboratory study was organized to determine repeatability and reproducibility of the determination of the median particle size and particle number concentration of Ag nanoparticles (AgNPs) in chicken meat. Ten laboratories from the European Union, the USA, and Canada determined particle size and particle number concentration of two chicken meat homogenates spiked with polyvinylpyrrolidone (PVP)-stabilized AgNPs. For the determination of the median particle diameter, repeatability standard deviations of 2 and 5% were determined, and reproducibility standard deviations were 15 and 25%, respectively. The equivalent median diameter itself was approximately 60% larger than the diameter of the particles in the spiking solution. Determination of the particle number concentration was significantly less precise, with repeatability standard deviations of 7 and 18% and reproducibility standard deviations of 70 and 90%.

  17. Efficacy of Artesunate + Sulphadoxine-Pyrimethamine (AS + SP and Amodiaquine + Sulphadoxine-Pyrimethamine (AQ + SP for Uncomplicated falciparum Malaria in Equatorial Guinea (Central Africa

    Directory of Open Access Journals (Sweden)

    Pilar Charle

    2009-01-01

    Full Text Available Objectives. The objectives of the study were (i to evaluate the efficacy of combination drugs, such as artesunate + sulphadoxine-pyrimethamine (AS + SP and amodiaquine + sulphadoxine-pyripethamine (AQ + SP in treatment of uncomplicated falciparum malaria (ii to differentiate recrudescence from reinfection by analysing msp-1 and msp-2 genes of Plasmodium falciparum in treatment failure cases. Methods. We carried out an in vivo study in the year 2005 in 206 children between 6 to 59 months age groups. Of the 206, 120 received AQ + SP, and 86 received AS + SP. A clinical and parasitological followup during 14 days was undertaken. Finger-prick blood sample from each patient was taken on Whatman filter paper (no. 3 on days 0, 7, 14 and also the day when the parasite and symptoms reappeared for PCR analysis. Results. Late treatment failure was observed in 3.5% (4/114 with AQ + SP, and 2.5% (2/79 with AS + SP. The success rate was 96.5% with AQ + SP and 97.5% with AS + SP. No deaths and severe reactions were recorded. Out of the 6 treatment failure cases, one was reinfection as observed by PCR analysis of msp-1 and msp-2 genes on day 14. Discussion. Both the combinations found to be efficacious and safe and could be used as a first-line treatment for uncomplicated falciparum malaria in Equatorial Guinea.

  18. SpF: Enabling Petascale Performance for Pseudospectral Dynamo Models

    Science.gov (United States)

    Jiang, W.; Clune, T.; Vriesema, J.; Gutmann, G.

    2013-12-01

    Pseudospectral (PS) methods possess a number of characteristics (e.g., efficiency, accuracy, natural boundary conditions) that are extremely desirable for dynamo models. Unfortunately, dynamo models based upon PS methods face a number of daunting challenges, which include exposing additional parallelism, leveraging hardware accelerators, exploiting hybrid parallelism, and improving the scalability of global memory transposes. Although these issues are a concern for most models, solutions for PS methods tend to require far more pervasive changes to underlying data and control structures. Further, improvements in performance in one model are difficult to transfer to other models, resulting in significant duplication of effort across the research community. We have developed an extensible software framework for pseudospectral methods called SpF that is intended to enable extreme scalability and optimal performance. High-level abstractions provided by SpF unburden applications of the responsibility of managing domain decomposition and load balance while reducing the changes in code required to adapt to new computing architectures. The key design concept in SpF is that each phase of the numerical calculation is partitioned into disjoint numerical 'kernels' that can be performed entirely in-processor. The granularity of domain-decomposition provided by SpF is only constrained by the data-locality requirements of these kernels. SpF builds on top of optimized vendor libraries for common numerical operations such as transforms, matrix solvers, etc., but can also be configured to use open source alternatives for portability. SpF includes several alternative schemes for global data redistribution and is expected to serve as an ideal testbed for further research into optimal approaches for different network architectures. In this presentation, we will describe the basic architecture of SpF as well as preliminary performance data and experience with adapting legacy dynamo codes

  19. Production of Cold Active Lipase from Bacillus sp.

    OpenAIRE

    Yasemin, Sara; Arabacı, Nihan; Korkmaz Güvenmez, Hatice

    2018-01-01

    A cold active lipase producing Bacillus sp. strains were isolated from sewage of oil. Bacillus sp. strain SY-7 was determined as the best lipase producing isolate. The highest enzyme production was found at 20°C and pH 8.0 on tributyrin media. Analyses of molecular mass of the partial purified lipase was carried out by SDS-PAGE which revealed a single band as 110.5 kDa. The enzyme activity and stability were determined by spectrophotometric and titrimetric methods. The enzyme was active betwe...

  20. Effect of tea saponin on ephyrae and polyps of the moon jellyfish Aurelia sp.1.

    Science.gov (United States)

    Dong, Zhijun; Sun, Tingting; Liang, Likun; Wang, Lei

    2017-01-01

    The moon jellyfish (Aurelia sp.1) is thought to be a nuisance for the sea cucumber aquaculture, which commonly occur in the sea cucumber (Apostichopus japonicus) culture ponds of the Yellow Sea, China. To develop an appropriate method to control Aurelia sp.1 blooms, the toxic effects of tea saponin on Aurelia sp.1 ephyrae and polyps were tested in laboratory experiments. Our results revealed that tea saponin caused significant morphological changes, behavioral abnormality and mortality in Aurelia sp.1 ephyrae and polyps in 24 h and 48 h exposure experiments. The 24 h and 48 h median lethal concentrations (LC50) values of tea saponin for Aurelia sp.1 ephyrae were 1.9 and 1.1 mg L-1 respectively, while the LC50 value for Aurelia sp.1 polyps was 0.4 mg L-1 after 24h and 48 h of exposure to tea saponin. Comparison with literature results of tea saponin on A. japonicus indicates that the resistance of A. japonicus to tea saponin is 12-18 times greater than that of Aurelia sp.1 ephyrae. Therefore, the appropriate tea saponin dosage for the control of Aurelia sp.1 should be paid enough attention in order to minimize possible damage for sea cucumber. We suggest that the recommended level of tea saponin to eradicate Aurelia sp.1 ephyrae and polyps in sea cucumber culture ponds be lower than 1.35 mg L-1.

  1. Effect of tea saponin on ephyrae and polyps of the moon jellyfish Aurelia sp.1.

    Directory of Open Access Journals (Sweden)

    Zhijun Dong

    Full Text Available The moon jellyfish (Aurelia sp.1 is thought to be a nuisance for the sea cucumber aquaculture, which commonly occur in the sea cucumber (Apostichopus japonicus culture ponds of the Yellow Sea, China. To develop an appropriate method to control Aurelia sp.1 blooms, the toxic effects of tea saponin on Aurelia sp.1 ephyrae and polyps were tested in laboratory experiments. Our results revealed that tea saponin caused significant morphological changes, behavioral abnormality and mortality in Aurelia sp.1 ephyrae and polyps in 24 h and 48 h exposure experiments. The 24 h and 48 h median lethal concentrations (LC50 values of tea saponin for Aurelia sp.1 ephyrae were 1.9 and 1.1 mg L-1 respectively, while the LC50 value for Aurelia sp.1 polyps was 0.4 mg L-1 after 24h and 48 h of exposure to tea saponin. Comparison with literature results of tea saponin on A. japonicus indicates that the resistance of A. japonicus to tea saponin is 12-18 times greater than that of Aurelia sp.1 ephyrae. Therefore, the appropriate tea saponin dosage for the control of Aurelia sp.1 should be paid enough attention in order to minimize possible damage for sea cucumber. We suggest that the recommended level of tea saponin to eradicate Aurelia sp.1 ephyrae and polyps in sea cucumber culture ponds be lower than 1.35 mg L-1.

  2. Neorickettsia risticii, Rickettsia sp. and Bartonella sp. in Tadarida brasiliensis bats from Buenos Aires, Argentina.

    Science.gov (United States)

    Cicuttin, Gabriel L; De Salvo, María N; La Rosa, Isabel; Dohmen, Federico E Gury

    2017-06-01

    Bats are potential reservoirs of many vector-borne bacterial pathogens. The aim of the present study was to detect species of Anaplasma, Ehrlichia, Neorickettsia, Rickettsia, Borrelia and Bartonella in Brazilian free-tailed bats (Tadarida brasiliensis, Molossidae) from Buenos Aires city, Argentina. Between 2012 and 2013, 61 T. brasiliensis from urban areas of Buenos Aires city were studied. The samples were molecularly screened by PCR and sequencing. Five bats (8.2%) were positive to Neorickettsia risticii, one (1.6%) was positive to Rickettsia sp. and three bats (4.9%) to Bartonella sp. For molecular characterization, the positive samples were subjected to amplification and sequencing of a fragment of p51 gene for N. risticii, a fragment of citrate synthase gene (gltA) for Rickettsia genus and a fragment of gltA for Bartonella genus. Phylogenetic tree was constructed using the maximum-likelihood method. Phylogenetic analysis of N. risticii detect in our study revealed that it relates to findings in the USA West Coast; Rickettsia sp. detected is phylogenetically within R. bellii group, which also includes many other Rickettsia endosymbionts of insects; and Bartonella sp. found is related to various Bartonella spp. described in Vespertilionidae bats, which are phylogenetically related to Molossidae. Our results are in accordance to previous findings, which demonstrate that insectivorous bats could be infected with vector-borne bacteria representing a potential risk to public health. Future research is necessary to clarify the circulation of these pathogens in bats from Buenos Aires. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Penicillium araracuarense sp. nov., Penicillium elleniae sp. nov., Penicillium penarojense sp. nov., Penicillium vanderhammenii sp. nov. and Penicillium wotroi sp. nov., isolated from leaf litter.

    Science.gov (United States)

    Houbraken, Jos; López-Quintero, Carlos A; Frisvad, Jens C; Boekhout, Teun; Theelen, Bart; Franco-Molano, Ana Esperanza; Samson, Robert A

    2011-06-01

    Several species of the genus Penicillium were isolated during a survey of the mycobiota of leaf litter and soil in Colombian Amazon forest. Five species, Penicillium penarojense sp. nov. (type strain CBS 113178(T) = IBT 23262(T)), Penicillium wotroi sp. nov. (type strain CBS 118171(T) = IBT 23253(T)), Penicillium araracuarense sp. nov. (type strain CBS 113149(T) = IBT 23247(T)), Penicillium elleniae sp. nov. (type strain CBS 118135(T) = IBT 23229(T)) and Penicillium vanderhammenii sp. nov. (type strain CBS 126216(T) = IBT 23203(T)) are described here as novel species. Their taxonomic novelty was determined using a polyphasic approach, combining phenotypic, molecular (ITS and partial β-tubulin sequences) and extrolite data. Phylogenetic analyses showed that each novel species formed a unique clade for both loci analysed and that they were most closely related to Penicillium simplicissimum, Penicillium janthinellum, Penicillium daleae and Penicillium brasilianum. An overview of the phylogeny of this taxonomically difficult group is presented, and 33 species are accepted. Each of the five novel species had a unique extrolite profile of known and uncharacterized metabolites and various compounds, such as penicillic acid, andrastin A, pulvilloric acid, paxillin, paspaline and janthitrem, were commonly produced by these phylogenetically related species. The novel species had a high growth rate on agar media, but could be distinguished from each other by several macro- and microscopical characteristics.

  4. Evaluation of biocontrol ability of native strains of Trichoderma spp on Rhizoctonia and Fusarium sp in coffee (Coffea arabica in experimental conditions

    Directory of Open Access Journals (Sweden)

    Nina Rudy

    2016-06-01

    Full Text Available Due to the indiscriminate use agrochemicals in conventional agriculture, it is causing pollution problems in the environment (soil, air and water, hence the search for alternatives that contribute to agricultural production by agro-chemical free sustainable production. This paper studies the biological control of damping off in coffee (Coffea arabica by applying antagonistic fungus Trichoderma sp. Under experimental conditions at laboratory facilities of the Academic Unit Carmen Pampa Campesina, a community of Carmen Pampa, Township Coroico. The aim of this study was to biologically control the "damping off", they found two genera that cause damping off in seedbed of coffee: Rhizoctonia sp. and Fusarium sp.To determine the percentage of growth and control in the culture medium, we used the method of counting quarters, where they gave the mycelial growth of antagonistic fungus Trichoderma sp., And the fungal pathogens Rhizoctonia sp. and Fusarium sp. Statistically there was a highly significant difference in the variable growth rate of Trichoderma sp. on pathogenic fungi Rhizoctonia sp. and Fusarium sp. at 3, 6 and 9 days that announces the time factor and treatments are interdependent. The control variable showed a highly significant difference in the time factor and treatment, but the interaction shows no significant difference this makes known factors that are independent, so the fungus Trichoderma sp. not depend on time in treatment, thus showing its inhibitory power to Rhizoctonia sp. and Fusarium sp .. This test gives references that there is antagonistic fungus control on the fungal pathogens Rhizoctonia sp. and Fusarium sp.

  5. Neues vom Heringsparasiten Ichthyophonus sp.

    OpenAIRE

    Lang, Thomas

    1994-01-01

    Seit dem Massensterben von Heringen entlang der schwedischen Südküste im Sommer 1991, aufgrund einer Infektion mit dem parasitischen Pilz Ichthyophonus sp., wird der Gesundheitszustand der Heringsbestände in den europäischen Seegebieten intensiv überwacht. Diese Untersuchungen, an denen sich auch die Bundesforschungsanstalt für Fischerei beteiligt, werden koordiniert von der "Arbeitsgruppe über Pathologie und Krankheiten mariner Organismen" des Internationalen Rates für Meeresforschung (IC...

  6. Curcumin and synthetic analogs induce reactive oxygen species and decreases specificity protein (Sp transcription factors by targeting microRNAs

    Directory of Open Access Journals (Sweden)

    Gandhy Shruti U

    2012-11-01

    Full Text Available Abstract Background Curcumin inhibits growth of several cancer cell lines, and studies in this laboratory in bladder and pancreatic cancer cells show that curcumin downregulates specificity protein (Sp transcription factors Sp1, Sp3 and Sp4 and pro-oncogenic Sp-regulated genes. In this study, we investigated the anticancer activity of curcumin and several synthetic cyclohexanone and piperidine analogs in colon cancer cells. Methods The effects of curcumin and synthetic analogs on colon cancer cell proliferation and apoptosis were determined using standardized assays. The changes in Sp proteins and Sp-regulated gene products were analysed by western blots, and real time PCR was used to determine microRNA-27a (miR-27a, miR-20a, miR-17-5p and ZBTB10 and ZBTB4 mRNA expression. Results The IC50 (half-maximal values for growth inhibition (24 hr of colon cancer cells by curcumin and synthetic cyclohexanone and piperidine analogs of curcumin varied from 10 μM for curcumin to 0.7 μM for the most active synthetic piperidine analog RL197, which was used along with curcumin as model agents in this study. Curcumin and RL197 inhibited RKO and SW480 colon cancer cell growth and induced apoptosis, and this was accompanied by downregulation of specificity protein (Sp transcription factors Sp1, Sp3 and Sp4 and Sp-regulated genes including the epidermal growth factor receptor (EGFR, hepatocyte growth factor receptor (c-MET, survivin, bcl-2, cyclin D1 and NFκB (p65 and p50. Curcumin and RL197 also induced reactive oxygen species (ROS, and cotreatment with the antioxidant glutathione significantly attenuated curcumin- and RL197-induced growth inhibition and downregulation of Sp1, Sp3, Sp4 and Sp-regulated genes. The mechanism of curcumin-/RL197-induced repression of Sp transcription factors was ROS-dependent and due to induction of the Sp repressors ZBTB10 and ZBTB4 and downregulation of microRNAs (miR-27a, miR-20a and miR-17-5p that regulate these repressors

  7. Suicide in Sorocaba-SP: an epidemiological study

    Directory of Open Access Journals (Sweden)

    Melissa Nobrega Vasques de Freitas

    2013-09-01

    Introduction: suicide is a major worldwide public health issue and appears as one of the ten most frequent causes of death in all ages. Objective: to perform an epidemiological analysis of suicide rates recorded between 2000 and 2009 in the city of Sorocaba-SP and compare them to national epidemiological data. Methods: We performed a descriptive analysis of suicide data in Sorocaba-SP in the period 2000 to 2009 collected by the Surveillance of Violence and Injuries (VIVA and the Municipal Health Secretariat of Sorocaba-SP. Results: a total of 229 suicide cases were recorded between 2000 and 2009. Total suicide rate suffered an increase of 3.8% in 2005, as compared to previous years, and remained constant in subsequent years. The highest average rates were recorded in 2005 and 2007. Men are more likely to commit suicide. The highest rates of suicide were recorded in the age group 35-44 years. Being single was the most common sociodemographic characteristic of suicides during the study period. The most common methods of suicide were hanging and firearms. Conclusion: The rate of suicide in Sorocaba-SP, despite low, is consistent with the national and international growth trends. Members of the younger population are killing themselves with increasing frequency, although the highest rates of suicide are still found among those aged 35 - 44 years

  8. Lung Surfactant Protein D (SP-D) Response and Regulation During Acute and Chronic Lung Injury

    DEFF Research Database (Denmark)

    Gaunsbaek, Maria Quisgaard; Rasmussen, Karina Juhl; Beers, Michael F.

    2013-01-01

    in three murine models of lung injury, using a validated ELISA technology for estimation of SP-D levels. METHODS: Mice were exposed to lipopolysaccharide, bleomycin, or Pneumocystis carinii (Pc) and sacrificed at different time points. RESULTS: In lipopolysaccharide-challenged mice, the level of SP...... injury, with a sustained increment during chronic inflammation compared with acute inflammation. A quick upregulation of SP-D in serum in response to acute airway inflammation supports the notion that SP-D translocates from the airways into the vascular system, in favor of being synthesized systemically....... The study also confirms the concept of using increased SP-D serum levels as a biomarker of especially chronic airway inflammation....

  9. Predicting fuel performance for SP-100 conditions

    International Nuclear Information System (INIS)

    Baars, R.E.

    1985-01-01

    This paper reports on methods for analyzing fuel designs proposed for the thermionic and thermoelectric concepts for SP-100 application. The proposed fuel design for the thermionic concept consisted of fully-enriched oxide fuel clad in chemical vapor deposition (CVD) tungsten, which also served as the emitter for the thermionic fuel element (TFE). The fuel density was 95% of theoretical with the linear heat rate flattened radially by removing fuel from the center of the fuel pellet. The fuel inner diameter varied from approx.0.45 in. at the core center to zero at the edge of the core. The as-fabricated gap between fuel and emitter was 10 mils radial. The emitter thickness was 80 mils, and the outer diameter was 1.099 in. The LIFE-4 code was used for evaluation of this concept after extensive review of the code and development of a procedure that corrects certain deficiencies noted in analysis of several tests

  10. Occurrence of “Babesia" sp. in crossbred calves by diagnosis methods in Campos dos Goytacazes, RJ, Brazil Ocorrência de "Babesia" sp. em bezerros mestiços, por meio de testes sorológicos, em Campos dos Goytacazes, RJ, Brasil

    Directory of Open Access Journals (Sweden)

    Maria Angélica Vieira da Costa Pereira

    2009-03-01

    Full Text Available 305 dairy calves sera samples at different ages for a serological survey in order to determine antibodies serum prevalence against Babesisa bovis and Babesia bigemina in zebu cattle as well as the crossbreds in Campos dos Goytacazes, RJ, Brazil were collected. By Indirect Fluorescent Antibody Test (IFAT and Indirect Enzyme-Linked Immunoadsorbent Assay (ELISA data were analyzed. Positive serum dairy cattle average percentages indicated 90.20 and 90.50% for B. bovis and 88.20 and 78.70% for B. bigemina by IFAT and ELISA, respectively. Both B. bovis and B. bigemina tests agreement ratio were 98.00 and 61.00%. Serum prevalence showed that the region is enzootically considered stable for bovine babesiosis infection with high disease prevalence. In Campos dos Goytacazes region whose livestock activity has been noted as a second income supporting, the present study may be regarding as unpublished, at all.Com o objetivo de realizar um levantamento soroepidemiológico para B. bovis e B. bigemina em bezerros de raças zebuínas e seus cruzamentos no município de Campos dos Goytacazes, RJ, foram coletadas 305 amostras de soros de bezerros de diferentes faixas etárias de propriedades dedicadas à produção leiteira. Os dados foram analisados pelas técnicas de imunofluorescência indireta (IFI e prova de imunoadsorção enzimática (ELISA. As médias dos percentuais obtidos de bovinos com sorologia positiva para B. bovis e B. bigemina foram de 90,20 e 90,50% e de 88,20 e 78,70% para IFI e ELISA, respectivamente. O grau de concordância dos testes para B. bovis foi de 98,00% e o de B. bigemina, de 61,00%. A soroprevalência encontrada caracteriza esta região como uma área de estabilidade enzoótica para B. bovis, com índice elevado na prevalência de portadores de Babesia sp. O presente trabalho é inédito na região de Campos dos Goytacazes, que tem a pecuária como segunda fonte de renda.

  11. Corrosive Metabolic Activity of Desulfovibrio sp. on 316L Stainless Steel

    Science.gov (United States)

    Arkan, Simge; Ilhan-Sungur, Esra; Cansever, Nurhan

    2016-12-01

    The present study investigated the effects of chemical parameters (SO4 2-, PO4 3-, Cl-, pH) and the contents of extracellular polymeric substances (EPS) regarding the growth of Desulfovibrio sp. on the microbiologically induced corrosion of 316L stainless steel (SS). The experiments were carried out in laboratory-scaled test and control systems. 316L SS coupons were exposed to Desulfovibrio sp. culture over 720 h. The test coupons were removed at specific sampling times for enumeration of Desulfovibrio sp., determination of the corrosion rate by the weight loss measurement method and also for analysis of carbohydrate and protein in the EPS. The chemical parameters of the culture were also established. Biofilm/film formation and corrosion products on the 316L SS surfaces were investigated by scanning electron microscopy and energy-dispersive x-ray spectrometry analyses in the laboratory-scaled systems. It was found that Desulfovibrio sp. led to the corrosion of 316L SS. Both the amount of extracellular protein and chemical parameters (SO4 2- and PO4 3-) of the culture caused an increase in the corrosion of metal. There was a significantly positive relationship between the sessile and planktonic Desulfovibrio sp. counts ( p < 0.01). It was detected that the growth phases of the sessile and planktonic Desulfovibrio sp. were different from each other and the growth phases of the sessile Desulfovibrio sp. vary depending on the subspecies of Desulfovibrio sp. and the type of metal when compared with the other published studies.

  12. Introduction to the SP theory of intelligence

    OpenAIRE

    Wolff, J Gerard

    2018-01-01

    This article provides a brief introduction to the "Theory of Intelligence" and its realisation in the "SP Computer Model". The overall goal of the SP programme of research, in accordance with long-established principles in science, has been the simplification and integration of observations and concepts across artificial intelligence, mainstream computing, mathematics, and human learning, perception, and cognition. In broad terms, the SP system is a brain-like system that takes in "New" infor...

  13. Antifungal activity of four honeys of different types from Algeria against pathogenic yeast: Candida albicans and Rhodotorula sp.

    OpenAIRE

    Ahmed Moussa; Djebli Noureddine; Aissat Saad; Meslem Abdelmelek; Benhalima Abdelkader

    2012-01-01

    Objective: To evaluate the antifungal activity of four honeys of different types from Algeria against pathogenic yeast i.e. Candida albicans (C. albicans) and Rhodotorula sp. Methods: Four Algeria honeys of different botanical origin were analyzed to test antifungal effect against C. albicans, and Rhodotorula sp. Different concentrations (undiluted, 10%, 30%, 50% and 70% w/v) of honey were studied in vitro for their antifugal activity using C. albicans and Rhodotorula sp. as fungal strains...

  14. Evaluation of antioxidant and cytotoxic properties of Cynobacteria, Limnothrix sp. and Leptolyngbya sp. from Arabian sea

    Digital Repository Service at National Institute of Oceanography (India)

    Anas, A.; Vinothkumar, S.; Gupta, S.; Jasmin, C.; Joseph, V.; Parameswaran, P.S.; Nair, S.

    The hexane fractions of the marine cyanobacteria: Leptolyngbya sp. and Limnothrix sp., collected from Arabian Sea were found to display promising antioxidant properties than their ethyl acetate fraction during radical scavenging ABTS/DPPH assays (IC...

  15. Xylanolytic enzyme systems in Arthrobacter sp MTCC 5214 and Lactobacillus sp.

    Digital Repository Service at National Institute of Oceanography (India)

    Khandeparker, R.; Jalal, T.

    The production of extracellular xylanolytic enzymes such as xylanase, alfa-L-arabinofuranosidase (alfa-l-AFase), and acetyl xylan esterase (Axe) by marine Arthrobacter sp and Lactobacillus sp was investigated using different carbon sources Induction...

  16. SpEnD: Linked Data SPARQL Endpoints Discovery Using Search Engines

    OpenAIRE

    Yumusak, Semih; Dogdu, Erdogan; Kodaz, Halife; Kamilaris, Andreas

    2016-01-01

    In this study, a novel metacrawling method is proposed for discovering and monitoring linked data sources on the Web. We implemented the method in a prototype system, named SPARQL Endpoints Discovery (SpEnD). SpEnD starts with a "search keyword" discovery process for finding relevant keywords for the linked data domain and specifically SPARQL endpoints. Then, these search keywords are utilized to find linked data sources via popular search engines (Google, Bing, Yahoo, Yandex). By using this ...

  17. Geoelectrical Methods and Monitoring for Dam Safety Assessment, Republic of Korea

    Science.gov (United States)

    Lim, S. K.; Oldenburg, D.; Kang, S.; Song, S. H.

    2016-12-01

    Geoelectrical methods and monitoring to detect the seepage and internal erosion are essential for the safety assessment of earth dams. This work aims to develop improved methodologies to analyze the observed data and to monitor changes in seepage flow using direct current (DC) and self-potential (SP) methods. The seasonal variation of water level at dams causes a change in seepage and water saturation and hence alters the resistivity of the dam material. DC data are sensitive to water saturation and hence changes in saturation can be obtained by repeatedly measuring DC data. However, a more diagnostic parameter for safety assessment is fluid flow, and resistivity is only weakly coupled to that. Fortunately SP signals are directly related to fluid flow, and thus an SP survey has the potential to characterize fluid flow through the earth matrix. In Korea, the safety assessment of earth fill dams has been dealt by Korea Rural Community Corporation (KRC). Most of the dams are relatively old ( >50 years), hence assessing deterioration and corresponding seepage of those dams are crucial. In order to evaluate the engineering geological properties of the soil at earth dams in Korea, two boreholes in each dam were drilled to a bedrock depth that exceeds the height of the dam. A large set of field tests, including standard penetration tests (SPT) and in-situ permeability tests, were carried out along the boreholes. However, seepage paths in the dam is complex hence those limited measurements at a few points is not sufficient to delineate the zone of preferential seepage flow. For this, KRC developed permanent DC monitoring systems at a number of agricultural dams in Korea. The data were automatically collected every 6 hours. During the monitoring, the measurements of the water level at two boreholes were gathered at the same time. In this presentation we select an agricultural dam and delineate an anomalous leakage zone by inverting and interpreting time-lapse DC resistivity

  18. [Influence of staphylococcin T on Enterococcus sp. growth].

    Science.gov (United States)

    Białucha, Agata; Kozuszko, Sylwia; Gospodarek, Eugenia; Bugalski, Roman Marian; Gierlotka, Krzysztof

    2007-01-01

    Bacteriocins are ribosomally synthesised, extracellular bacterial products. Generally, spectrum of inhibition is limited to the same or closely related species to bacteriocin producer. Staphylococcin T is produced by Staphylococcus cohnii strain. The present study concerns influence of StT to 267 Enterococcus sp. strains growth isolated between 2003 and 2006 in Department of Microbiology University Hospital of dr. A. Jurasz in Bydgoszcz. S. cohnii T antagonistic ability evaluated towards bacteries on Mueller-Hinton Agar (bio Mérieux) in aerobic conditions. After 24 and 48 hours tested enterococci suspensions were plated perpendiculary. Susceptibility to antibiotics was assessed by disc diffusion method according to the guideless of Clinical and Laboratory Standards Institute and National Reference Centre for Antimicrobial Susceptibility. Among Enterococcus sp. strains tested 7.1% were sensitive to StT. The highest percentage of sensitive enterococci isolated from wound swabs, urine, blood and pus. Enterococcus faecium strains dominated (63.2%) among enterococci sensitive to StT. Moderate inhibition degree on S. cohnii T bacteriocin action was observed in majority sensitive enterococci strains. Enterococcus sp. sensitive to StT strains were frequently multidrug resistant (68.4%). According to the study results and increasing resistance to antibiotics, StT could be an alternative agent used to treat infections caused by Enterococcus sp.

  19. Anaerobic Co-Digestion of the Microalgae Scenedesmus Sp.

    Energy Technology Data Exchange (ETDEWEB)

    Ramos-Suarez, J. L.; Carreras, N.

    2011-06-07

    Microalgae biomass has been widely studied for biogas production over the last years and results show that anaerobic digestion is often limited by the low C/N ratio of this type of biomass. Therefore, codigestion with substrates of high C/N ratio is necessary. The objectives of this study are to set up an experimental method that ease reproducibility and control of anaerobic digestion processes in laboratory conditions and to determine the biodegradability and biogas production potential of the co-digestion process of microalgae Scenedesmus sp. and energy crop Opuntia ficus indica (L.) Miller. Results obtained showed that higher C/N ratios are preferred in order to maximize methane production. Highest methane yield obtained was 0.252m3CH4/Kg VS and degradability expressed as percentage COD reduced is around 30% for the ideal mixture found, made up of 75% O. ficus-indica and 25% Scenedesmus sp. in VS basis. A laboratory setup using MicroOxymax respirometer, after its adaptation to work under anaerobic conditions, can be used for the monitoring of anaerobic digestion processes. Scenedesmus sp. as sole substrate for anaerobic digestion does not give good results due to low C/N ratio. However, when codigesting it with O. ficus-indica methane production is satisfactory. Best mixture was made up of 75% O. ficus-indica and 25% Scenedesmus sp. in VS basis. (Author)

  20. The Growth of Monoraphidium sp. and Scenedesmus sp. Cells in the Presence of Thorium

    Directory of Open Access Journals (Sweden)

    Juliana Cristina de Queiroz

    2012-01-01

    Full Text Available Toxicity of thorium by Monoraphidium sp. and Scenedesmus sp. was studied. Microalgal cultures were inoculated in ASM-1 medium in presence and absence of thorium. Its effect was monitored by direct counting on Fuchs-Rosenthal chamber and with software. The toxicity of thorium over the species was observed for concentrations over 50.0 mg/L. After 30 days, Monoraphidium cells decreased their concentration from 4.23×106 to 4.27×105 and 8.57×105 cells/mL, in the presence of 50.0 and 100.0 mg/L of thorium, respectively. Scenedesmus sp. cells were more resistant to thorium: for an initial cell concentration of 7.65×104 cells/mL it was observed a change to 5.25×105 and 5.12×105 cells/mL, in the presence of thorium at 50.0 and 100.0 mg/L, respectively. This is an indication that low concentrations of the radionuclide favored the growth, and that Scenedesmus cells are more resistant to thorium than Monoraphidium cells. The software used for comparison with direct count method proved to be useful for the improvement of accuracy of the results obtained, a decrease in the uncertainty and allowed recording of the data. The presence of thorium suggests that low concentrations have a positive effect on the growth, due to the presence of the nitrate, indicating its potential for ecotoxicological studies.

  1. The Growth of Monoraphidium sp. and Scenedesmus sp. Cells in the Presence of Thorium

    Science.gov (United States)

    de Queiroz, Juliana Cristina; Ferreira, Ana Cristina de Melo; da Costa, Antonio Carlos Augusto

    2012-01-01

    Toxicity of thorium by Monoraphidium sp. and Scenedesmus sp. was studied. Microalgal cultures were inoculated in ASM-1 medium in presence and absence of thorium. Its effect was monitored by direct counting on Fuchs-Rosenthal chamber and with software. The toxicity of thorium over the species was observed for concentrations over 50.0 mg/L. After 30 days, Monoraphidium cells decreased their concentration from 4.23 × 106 to 4.27 × 105 and 8.57 × 105 cells/mL, in the presence of 50.0 and 100.0 mg/L of thorium, respectively. Scenedesmus sp. cells were more resistant to thorium: for an initial cell concentration of 7.65 × 104 cells/mL it was observed a change to 5.25 × 105 and 5.12 × 105 cells/mL, in the presence of thorium at 50.0 and 100.0 mg/L, respectively. This is an indication that low concentrations of the radionuclide favored the growth, and that Scenedesmus cells are more resistant to thorium than Monoraphidium cells. The software used for comparison with direct count method proved to be useful for the improvement of accuracy of the results obtained, a decrease in the uncertainty and allowed recording of the data. The presence of thorium suggests that low concentrations have a positive effect on the growth, due to the presence of the nitrate, indicating its potential for ecotoxicological studies. PMID:22649297

  2. Pseudoxanthomonas koreensis sp. nov. and Pseudoxanthomonas daejeonensis sp. nov.

    Science.gov (United States)

    Yang, Deok-Chun; Im, Wan-Taek; Kim, Myung Kyum; Lee, Sung-Taik

    2005-03-01

    Gram-negative, non-spore-forming, rod-shaped bacteria, T7-09(T) and TR6-08(T), were isolated from soil from a ginseng field in South Korea and characterized to determine their taxonomic position. 16S rRNA gene sequence analysis showed that the two isolates shared 99.5 % sequence similarity. Strains T7-09(T) and TR6-08(T) were shown to belong to the Proteobacteria and showed the highest levels of sequence similarity to Pseudoxanthomonas broegbernensis DSM 12573(T) (98.1 %), Pseudoxanthomonas mexicana AMX 26B(T) (97.4-97.5 %), Pseudoxanthomonas japonensis 12-3(T) (96.5-96.6 %), Pseudoxanthomonas taiwanensis ATCC BAA-404(T) (95.7 %) and Xanthomonas campestris ATCC 33913(T) (96.3-96.5 %). The sequence similarity values with respect to any species with validly published names in related genera were less than 96.5 %. The detection of a quinone system with Q-8 as the predominant compound and a fatty acid profile with C(15 : 0) iso as the predominant acid supported the assignment of the novel isolates to the order 'Xanthomonadales'. The two isolates could be distinguished from the established species of the genus Pseudoxanthomonas by the presence of quantitative unsaturated fatty acid C(17 : 1) iso omega9c and by their unique biochemical profiles. The results of DNA-DNA hybridization clearly demonstrated that T7-09(T) and TR6-08(T) represent separate species. On the basis of these data, it is proposed that T7-09(T) (=KCTC 12208(T)=IAM 15116(T)) and TR6-08(T) (=KCTC 12207(T)=IAM 15115(T)) be classified as the type strains of two novel Pseudoxanthomonas species, for which the names Pseudoxanthomonas koreensis sp. nov. and Pseudoxanthomonas daejeonensis sp. nov., respectively, are proposed.

  3. Specialised predation by Palpimanus sp. (Araneae: Palpimanidae ...

    African Journals Online (AJOL)

    This is the first detailed report on the natural prey and the prey-capture tactics of a Palpimanus sp. from Entebbe (Uganda). Although this species fed occasionally on insects, its dominant prey in the field was other spiders, especially jumping spiders (Salticidae) and their eggs. Encounters between Palpimanus sp. and ...

  4. Kultivasi Scenedesmus SP. Pada Medium Air Limbah

    OpenAIRE

    Kawaroe, Mujizat

    2011-01-01

    Proses fotosintesis pada mikroalga membutuhkan CO2 dan cahaya matahari serta nutrien untuk pertumbuhannya. Kultivasi Scenedesmus sp. pada medium air limbah bertujuan guna mencukupi kebutuhan mikroalga akan nutrien dan mengurangi masukan dari bahan kimia yang terkandung dalam air limbah tersebut ke lingkungan. Kultivasi Scenedesmus sp. dilakukan selama tujuh hari pada medium air limbah industri tanpa penambahan nutri...

  5. Presence of potentially pathogenic Babesia sp. for human in Ixodes ricinus in Switzerland.

    Science.gov (United States)

    Casati, Simona; Sager, Heinz; Gern, Lise; Piffaretti, Jean-Claude

    2006-01-01

    We have designed and performed a new PCR method based on the 18S rRNA in order to individuate the presence and the identity of Babesia parasites. Out of 1159 Ixodes ricinus (Acari: Ixodidae) ticks collected in four areas of Switzerland, nine were found to contain Babesia DNA. Sequencing of the short amplicon obtained (411-452 bp) allowed the identification of three human pathogenic species: Babesia microti, B. divergens, for the first time in Switzerland, Babesia sp. EU1. We also report coinfections with B. sp. EU1-Borrelia burgdorferi sensu stricto and Babesia sp. EU1-B. afzelii.

  6. Guide til gode spørgeskemaer

    DEFF Research Database (Denmark)

    Olsen, Henning

    Spørgeskemaundersøgelser bliver ofte brugt til at dokumentere forskellige forhold og begrunde politiske beslutninger. Men resultaterne kan være forbundet med stor usikkerhed. Det kræver omhu og stor sproglig formåen at udarbejde spørgeskemaer. Seniorforsker Henning Olsen har i flere år arbejdet med...... viden om, hvordan folk forstår sproglige meddelelser og genkalder sig informationer. I guiden behandles emner som fx styrende problemstillinger og spørgsmåls fokus og neutralitet, formulering af åbne eller lukkede spørgsmål og svarkategorier, tematiske spørgeforløb, aflastning af svarpersoners...

  7. Glyphosate catabolism by Pseudomonas sp

    International Nuclear Information System (INIS)

    Shinabarger, D.L.

    1986-01-01

    The pathway for the degradation of glyphosate (N-phosphonomethylglycine) by Pseudomonas sp. PG2982 has been determined using metabolic radiolabeling experiments. Radiorespirometry experiments utilizing [3- 14 C] glyphosate revealed that approximately 50-59% of the C3 carbon was oxidized to CO 2 . Fractionation of stationary phase cells labeled with [3- 14 C]glyphosate revealed that from 45-47% of the assimilated C3 carbon is distributed to proteins and that amino acids methionine and serine are highly labeled. The nucleic acid bases adenine and guanine received 90% of the C3 label that was incorporated into nucleic acids, and the only pyrimidine base labeled was thymine. Pulse labeling of PG2982 cells with [3- 14 C]glyphosate revealed that [3- 14 C]sarcosine is an intermediate in glyphosate degradation. Examination of crude extracts prepared from PG2982 cells revealed the presence of an enzyme that oxidizes sarcosine to glycine and formaldehyde. These results indicate that the first step in glyphosate degradation by PG2982 is cleavage of the carbon-phosphorus bond, resulting in the release of sarcosine and a phosphate group. The phosphate group is utilized as a source of phosphorus, and the sarcosine is degraded to glycine and formaldehyde. Phosphonate utilization by Pseudomonas sp. PG2982 was investigated. Each of the ten phosphonates tested were utilized as a sole source of phosphorus by PG2982. Representative compounds tested included alkylphosphonates, 1-amino-substituted alkylphosphonates, amino-terminal phosphonates, and an arylphosphonate. PG2982 cultures degraded phenylphosphonate to benzene and produced methane from methylphosphonate. The data indicate that PG2982 is capable of cleaving the carbon-phosphorus bond of several structurally different phosphonates

  8. Listeria booriae sp. nov. and Listeria newyorkensis sp. nov., from food processing environments in the USA.

    Science.gov (United States)

    Weller, Daniel; Andrus, Alexis; Wiedmann, Martin; den Bakker, Henk C

    2015-01-01

    Sampling of seafood and dairy processing facilities in the north-eastern USA produced 18 isolates of Listeria spp. that could not be identified at the species-level using traditional phenotypic and genotypic identification methods. Results of phenotypic and genotypic analyses suggested that the isolates represent two novel species with an average nucleotide blast identity of less than 92% with previously described species of the genus Listeria. Phylogenetic analyses based on whole genome sequences, 16S rRNA gene and sigB gene sequences confirmed that the isolates represented by type strain FSL M6-0635(T) and FSL A5-0209 cluster phylogenetically with Listeria cornellensis. Phylogenetic analyses also showed that the isolates represented by type strain FSL A5-0281(T) cluster phylogenetically with Listeria riparia. The name Listeria booriae sp. nov. is proposed for the species represented by type strain FSL A5-0281(T) ( =DSM 28860(T) =LMG 28311(T)), and the name Listeria newyorkensis sp. nov. is proposed for the species represented by type strain FSL M6-0635(T) ( =DSM 28861(T) =LMG 28310(T)). Phenotypic and genotypic analyses suggest that neither species is pathogenic. © 2015 IUMS.

  9. Trichoderma sp. dalam Pengendalian Penyakit Layu Fusarium pada Tanaman Tomat

    OpenAIRE

    Novita, Trias

    2013-01-01

    Penelitian ini bertujuan untuk mengetahui peran Trichoderma sp dalam pengendalianpenyakit layu fusarium pada tanaman tomat. Penelitian dilaksanakan di Rumah Kaca FakultasPertanian Universitas Jambi, perlakuannya terdiri dari : t0 = tanpa Trichoderma sp; t1 = 25 gTrichoderma sp/8 kg media; t2 = 50 g Trichoderma sp/8 kg media; t3 = 75 g Trichoderma sp/8 kgmedia; dan t4 = 100 g Trichoderma sp /8 kg media. Hasil penelitian menunjukkan bahwa Trichodermasp berperan dalam mengendalikan penyakit layu...

  10. INTERAKSI ANTARA Trichoderma Harzianum, Penicillium SP. DAN Pseudomonas SP. SERTA KAPASITAS ANTAGONISMENYA TERHADAP Phytophthora CapsicilN VITRO*[Interaction Among Trichoderma Harzianum, Penicillium SP., Pseudomonas SP. and Antagonism Capacities Against Phy

    OpenAIRE

    Suharna, Nandang

    2003-01-01

    A preliminary study has been done to know antagonism capacities of three isolates of Trichoderma harzianum, two isolates of Penicillium sp.and one isolate of Pseudomonas sp.against Phytophthora capsici in vitro and interaction among those six antagonists.The highest antagonism capacity possessed by Penicillium sp. KN1, respectively followed by Penicillium sp.KN2,Pseudomonas sp. GH1 and the three T. harzianum isolates. Except for those three T. harzianum isolates, the two Penicillium sp.isolat...

  11. SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis

    Directory of Open Access Journals (Sweden)

    Mario Saare

    2015-01-01

    Full Text Available The SP100 family members comprise a set of closely related genes on chromosome 2q37.1. The widely expressed SP100 and the leukocyte-specific proteins SP110 and SP140 have been associated with transcriptional regulation and various human diseases. Here, we have characterized the SP100 family member SP140L. The genome sequence analysis showed the formation of SP140L gene through rearrangements of the two neighboring genes, SP100 and SP140, during the evolution of higher primates. The SP140L expression is interferon-inducible with high transcript levels in B cells and other peripheral blood mononuclear cells. Subcellularly, SP140L colocalizes with SP100 and SP140 in nuclear structures that are devoid of SP110, PML, or p300 proteins. Similarly to SP100 and SP140 protein, we detected serum autoantibodies to SP140L in patients with primary biliary cirrhosis using luciferase immunoprecipitation system and immunoblotting assays. In conclusion, our results show that SP140L is phylogenetically recent member of SP100 proteins and acts as an autoantigen in primary biliary cirrhosis patients.

  12. [Secondary metabolites of a marine mangrove fungus (Penicillium sp. no. 2556) from South China Sea].

    Science.gov (United States)

    Li, Chun-Yuan; Ding, Wei-Jia; Shao, Chang-Lun; She, Zhi-Gang; Lin, Yong-Cheng

    2008-07-01

    The metabolites of a marine mangrove fungus (Penicillium sp. No. 2556) were studied in this paper and six compounds were isolated from the fermentation liquid. Their structures were elucidated by spectroscopy methods as Sch54796 (1), Sch54794 (2), 4-hydroxybenzoic acid (3), urail (4), succinic acid (5), Vermopyrone (6). Among them, compounds 1, 2 and 6 were firstly isolated from Penicillium sp., Coumpounds 1 and 2 remarkably inhibited the growth of cancer cell lines hep2 and hepG2.

  13. Antifungal Activity of Lactobacillus sp. Bacteria in the Presence of Xylitol and Galactosyl-Xylitol

    OpenAIRE

    Lipi?ska, Lidia; Klewicki, Robert; Klewicka, El?bieta; Ko?odziejczyk, Krzysztof; S?jka, Micha?; Nowak, Adriana

    2016-01-01

    Lactic acid fermentation is a natural method of antimicrobial food protection. Antagonistic activity of Lactobacillus sp. bacteria, taking part in this process, is directed mainly against the same or other microorganisms. In this work we determine the impact of the presence of xylitol and galactosyl-xylitol on the antagonistic activity of 60 Lactobacillus sp. strains against indicator molds (Alternaria alternata, Alternaria brassicicola, Aspergillus niger, Fusarium latenicum, Geotrichum candi...

  14. Ligand-Promoted C(sp(3) )-H Olefination en Route to Multi-functionalized Pyrazoles.

    Science.gov (United States)

    Yang, Weibo; Ye, Shengqing; Schmidt, Yvonne; Stamos, Dean; Yu, Jin-Quan

    2016-05-17

    A Pd-catalyzed/N-heterocycle-directed C(sp(3) )-H olefination has been developed. The monoprotected amino acid ligand (MPAA) is found to significantly promote Pd-catalyzed C(sp(3) )-H olefination for the first time. Cu(OAc)2 instead of Ag(+) salts are used as the terminal oxidant. This reaction provides a useful method for the synthesis of alkylated pyrazoles. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource

    OpenAIRE

    Srivastava, Akanksha; Tiwari, Ratnakar; Srivastava, Vikas; Singh, Tej Bali; Asthana, Ravi Kumar

    2015-01-01

    An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732) were isolated (paddy fields and ponds in the Banaras Hindu University, campus) and five strains screened for ant...

  16. The role of heterotrophic microorganism Galactomyces sp. Z3 in improving pig slurry bioleaching.

    Science.gov (United States)

    Zhou, Jun; Zheng, Guanyu; Zhou, Lixiang; Liu, Fenwu; Zheng, Chaocheng; Cui, Chunhong

    2013-01-01

    The feasibility of removing heavy metals and eliminating pathogens from pig slurry through bioleaching involving the fungus Galactomyces sp. Z3 and two acidophilic thiobacillus (A. ferrooxidans LX5 and A. thiooxidans TS6) was investigated. It was found that the isolated pig slurry dissolved organic matter (DOM) degrader Z3 was identified as Galactomyces sp. Z3, which could grow well at pH 2.5-7 and degrade pig slurry DOM from 1973 to 942 mg/l within 48 h. During the successive multi-batch bioleaching systems, the co-inoculation of pig slurry degrader Galactomyces sp. Z3 and the two Acidithiobacillus species could improve pig slurry bioleaching efficiency compared to the single system without Galactomyces sp. Z3. The removal efficiency of Zn and Cu exceeded 94% and 85%, respectively. In addition, the elimination efficiencies of pathogens, including both total coliform and faecal coliform counts, exceeded 99% after bioleaching treatment. However, the counts of Galactomyces sp. Z3 decreased with the fall of pH and did not restore to the initial level during successive multi-batch bioleaching systems, and it is necessary to re-inoculate Galactomyces sp. Z3 cells into the bioleaching system to maintain its role in degrading pig slurry DOM. Therefore, a bioleaching technique involving both Galactomyces sp. Z3 and Acidithiobacillus species is an efficient method for removing heavy metals and eliminating pathogens from pig slurry.

  17. An assessment of adaptive and antagonistic properties of Trichoderma sp. strains in vegetable waste composts

    Directory of Open Access Journals (Sweden)

    Wolna-Maruwka Agnieszka

    2017-12-01

    Full Text Available The experiment consisted in monitoring the count of moulds and three selected Trichoderma sp. isolates (T1 - Trichoderma atroviride, T2 - Trichoderma harzianum, T3 - Trichoderma harzianum in vegetable (onion and tomato waste composted with additives (straw, pig manure. Additionally, the aim of the study was to determine the type of interaction occurring between autochthonous fungi isolated from composts after the end of the thermophilic phase and Trichoderma sp. strains applied in the experiment. Number of microorganisms was determined by the plate method, next the identification was confirmed. The rating scale developed by Mańka was used to determine the type of interactions occurring between microorganisms. The greatest count of moulds in onion waste composts was noted in the object which had simultaneously been inoculated with two strains T1 - T. atroviride and T3 - T. harzianum. The greatest count of moulds was noted in the tomato waste composts inoculated with T2 - T. harzianum strain. Microscope identification revealed that Penicillum sp., Rhizopus sp., Alternaria sp. and Mucor sp. strains were predominant in onion waste composts. In tomato waste composts Penicillium was the predominant genus, followed by Rhizopus. The test of antagonism revealed the inhibitory effect of Trichoderma isolates on most autochthonous strains of moulds. Tomato waste composts proved to be better substrates for the growth and development of Trichoderma sp. isolates. The results of the study show that vegetable waste can be used in agriculture as carriers of antagonistic microorganisms.

  18. "SP-G", a putative new surfactant protein--tissue localization and 3D structure.

    Directory of Open Access Journals (Sweden)

    Felix Rausch

    Full Text Available Surfactant proteins (SP are well known from human lung. These proteins assist the formation of a monolayer of surface-active phospholipids at the liquid-air interface of the alveolar lining, play a major role in lowering the surface tension of interfaces, and have functions in innate and adaptive immune defense. During recent years it became obvious that SPs are also part of other tissues and fluids such as tear fluid, gingiva, saliva, the nasolacrimal system, and kidney. Recently, a putative new surfactant protein (SFTA2 or SP-G was identified, which has no sequence or structural identity to the already know surfactant proteins. In this work, computational chemistry and molecular-biological methods were combined to localize and characterize SP-G. With the help of a protein structure model, specific antibodies were obtained which allowed the detection of SP-G not only on mRNA but also on protein level. The localization of this protein in different human tissues, sequence based prediction tools for posttranslational modifications and molecular dynamic simulations reveal that SP-G has physicochemical properties similar to the already known surfactant proteins B and C. This includes also the possibility of interactions with lipid systems and with that, a potential surface-regulatory feature of SP-G. In conclusion, the results indicate SP-G as a new surfactant protein which represents an until now unknown surfactant protein class.

  19. Method

    Directory of Open Access Journals (Sweden)

    Ling Fiona W.M.

    2017-01-01

    Full Text Available Rapid prototyping of microchannel gain lots of attention from researchers along with the rapid development of microfluidic technology. The conventional methods carried few disadvantages such as high cost, time consuming, required high operating pressure and temperature and involve expertise in operating the equipment. In this work, new method adapting xurography method is introduced to replace the conventional method of fabrication of microchannels. The novelty in this study is replacing the adhesion film with clear plastic film which was used to cut the design of the microchannel as the material is more suitable for fabricating more complex microchannel design. The microchannel was then mold using polymethyldisiloxane (PDMS and bonded with a clean glass to produce a close microchannel. The microchannel produced had a clean edge indicating good master mold was produced using the cutting plotter and the bonding between the PDMS and glass was good where no leakage was observed. The materials used in this method is cheap and the total time consumed is less than 5 hours where this method is suitable for rapid prototyping of microchannel.

  20. Enhancement of sp3 hybridized C in amorphous carbon films by Ar ion bombardment and Si incorporation

    International Nuclear Information System (INIS)

    Jung, Hae-Suk; Park, Hyung-Ho; Mendieta, I.R.; Smith, D.A.

    2003-01-01

    We report an effective method of increasing the sp 3 hybridization fraction in sputtered amorphous carbon (a-C) film by the combination of Ar ion bombardment and Si incorporation. In the deposition of an a-C film, Ar ion bombardment by controlling the applied bias voltage plays a role in creating high stress in film and causes the local bonding configuration to change to a sp 3 hybridized bond. Simultaneously, the incorporated Si in an a-C network breaks the sp 2 hybridized bonded ring and promotes the formation of a sp 3 hybridized bond. This enhancement of the sp 3 hybridized bonding characteristic is maximized for an a-C film with 23 at. % of Si and 100-150 V of applied bias voltage. In this region, the increase of resistivity, optical band gap, and mechanical hardness of a-C is attributed to the reduction of the sp 2 hybridized bonded ring and increased fraction of the sp 3 hybridized bond. However, at a higher bias voltage above 150 V, the enhancement effect is reduced due to the resputtering and thermally activated reconversion of a sp 3 to a sp 2 hybridized bond

  1. TurboSP and the Topological Trigger

    CERN Document Server

    Belavin, Vladislav

    2016-01-01

    TurboSP was originally proposed as an alternative to Full stream in LHCb data flow. TurboSP is a data flow strategy which not only selects events that should be preserved, like in Full stream, but also provides selective persistence. This is achieved by saving candidates and subset of the reconstruction. During this summer project we investigated the physics viability of using TurboSP with the topological lines and found out a possibility to reduce the number of kept tracks per event by two times while keeping a ratio of fully picked up interesting decay modes on $\\sim 97 \\%$ level.

  2. Geophysical Methods for Monitoring Temperature Changes in Shallow Low Enthalpy Geothermal Systems

    Directory of Open Access Journals (Sweden)

    Thomas Hermans

    2014-08-01

    Full Text Available Low enthalpy geothermal systems exploited with ground source heat pumps or groundwater heat pumps present many advantages within the context of sustainable energy use. Designing, monitoring and controlling such systems requires the measurement of spatially distributed temperature fields and the knowledge of the parameters governing groundwater flow (permeability and specific storage and heat transport (thermal conductivity and volumetric thermal capacity. Such data are often scarce or not available. In recent years, the ability of electrical resistivity tomography (ERT, self-potential method (SP and distributed temperature sensing (DTS to monitor spatially and temporally temperature changes in the subsurface has been investigated. We review the recent advances in using these three methods for this type of shallow applications. A special focus is made regarding the petrophysical relationships and on underlying assumptions generally needed for a quantitative interpretation of these geophysical data. We show that those geophysical methods are mature to be used within the context of temperature monitoring and that a combination of them may be the best choice regarding control and validation issues.

  3. Extraction of antioxidants from Chlorella sp. using subcritical water treatment

    Science.gov (United States)

    Zakaria, S. M.; Mustapa Kamal, S. M.; Harun, M. R.; Omar, R.; Siajam, S. I.

    2017-06-01

    Chlorella sp. microalgae is one of the main source of natural bioactive compounds used in the food and pharmaceutical industries. Subcritical water extraction is the technique that offers an efficient, non-toxic, and environmental-friendly method to obtain natural ingredients. In this work, the extracts of Chlorella sp. microalgae was evaluated in terms of: chemical composition, extraction (polysaccharides) yield and antioxidant activity, using subcritical water extraction. Extractions were performed at temperatures ranging from 100°C to 300°C. The results show that by using subcritical water, the highest yield of polysaccharides is 23.6 that obtained at 150°C. Analysis on the polysaccharides yield show that the contents were highly influenced by the extraction temperature. The individual antioxidant activity were evaluated by in vitro assay using a free radical method. In general, the antioxidant activity of the extracts obtained at different water temperatures was high, with values of 31.08-54.29 . The results indicated that extraction by subcritical water was effective and Chlorella sp. can be a useful source of natural antioxidants.

  4. method

    Directory of Open Access Journals (Sweden)

    L. M. Kimball

    2002-01-01

    Full Text Available This paper presents an interior point algorithm to solve the multiperiod hydrothermal economic dispatch (HTED. The multiperiod HTED is a large scale nonlinear programming problem. Various optimization methods have been applied to the multiperiod HTED, but most neglect important network characteristics or require decomposition into thermal and hydro subproblems. The algorithm described here exploits the special bordered block diagonal structure and sparsity of the Newton system for the first order necessary conditions to result in a fast efficient algorithm that can account for all network aspects. Applying this new algorithm challenges a conventional method for the use of available hydro resources known as the peak shaving heuristic.

  5. Detection of Salmonella sp., Vibrio sp. and total plate count bacteria on blood cockle (Anadara granosa)

    Science.gov (United States)

    Ekawati, ER; Yusmiati, S. N. H.

    2018-01-01

    Blood cockle (Anadara granosa) has high level of zinc and protein, which is beneficial for therapeutic function for malnourished particularly stunting case in children. Zinc in animal foods is more absorbable than that from vegetable food. Blood cockle (Anadara granosa) is rich in nutrient and an excellent environment for the growth of microorganisms. This research aimed to identify the contamination of Salmonella sp., Vibrio sp. and total plate count bacteria on blood cockle (Anadara granosa). This was observation research with laboratory analysis. Salmonella sp. and Vibrio sp. were detected from blood cockle. Total plate count was determine of the total amount of the bacteria. Results detected from 20 samples of blood cockle showed that all samples were negative of Salmonella sp. and 1 sample positive Vibrio sp. The result of total plate count bacteria was < 5 x 105 colony/g sample.

  6. Superhard sp2–sp3 hybrid carbon allotropes with tunable electronic properties

    Directory of Open Access Journals (Sweden)

    Meng Hu

    2016-05-01

    Full Text Available Four sp2–sp3 hybrid carbon allotropes are proposed on the basis of first principles calculations. These four carbon allotropes are energetically more favorable than graphite under suitable pressure conditions. They can be assembled from graphite through intralayer wrinkling and interlayer buckling, which is similar to the formation of diamond from graphite. For one of the sp2–sp3 hybrid carbon allotropes, mC24, the electron diffraction patterns match these of i-carbon, which is synthesized from shock-compressed graphite (H. Hirai and K. Kondo, Science, 1991, 253, 772. The allotropes exhibit tunable electronic characteristics from metallic to semiconductive with band gaps comparable to those of silicon allotropes. They are all superhard materials with Vickers hardness values comparable to that of cubic BN. The sp2–sp3 hybrid carbon allotroes are promising materials for photovoltaic electronic devices, and abrasive and grinding tools.

  7. New Eudesmane-Type Sesquiterpenoids from the Mangrove-Derived Endophytic Fungus Penicillium sp. J-54

    OpenAIRE

    Liuming Qiu; Pei Wang; Ge Liao; Yanbo Zeng; Caihong Cai; Fandong Kong; Zhikai Guo; Peter Proksch; Haofu Dai; Wenli Mei

    2018-01-01

    Four new eudesmane-type sesquiterpenoids, penicieudesmol A–D (1–4), were isolated from the fermentation broth of the mangrove-derived endophytic fungus Penicillium sp. J-54. Their structures were determined by spectroscopic methods, the in situ dimolybdenum CD method, and modified Mosher’s method. The bioassays results showed that 2 exhibited weak cytotoxicity against K-562 cells.

  8. New Eudesmane-Type Sesquiterpenoids from the Mangrove-Derived Endophytic Fungus Penicillium sp. J-54.

    Science.gov (United States)

    Qiu, Liuming; Wang, Pei; Liao, Ge; Zeng, Yanbo; Cai, Caihong; Kong, Fandong; Guo, Zhikai; Proksch, Peter; Dai, Haofu; Mei, Wenli

    2018-03-28

    Four new eudesmane-type sesquiterpenoids, penicieudesmol A-D ( 1 - 4 ), were isolated from the fermentation broth of the mangrove-derived endophytic fungus Penicillium sp. J-54. Their structures were determined by spectroscopic methods, the in situ dimolybdenum CD method, and modified Mosher's method. The bioassays results showed that 2 exhibited weak cytotoxicity against K-562 cells.

  9. EOP Gold Coral (Gerardia sp.) Growth Measurements

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Gold coral (Gerardia sp.) trees that were inspected years earlier on Pisces submersible dives were revisited and their change in size measured. The fishery for...

  10. Metabolites from marine fungus Aspergillus sp.

    Digital Repository Service at National Institute of Oceanography (India)

    Wahidullah, S.; Rajmanickam, R.; DeSouza, L.

    Chemical examination of a methanolic extract of the marine fungus, Aspergillus sp., isolated from marine grass environment, yielded a steroid, ergosterol peroxide (1), and a mixture of known glyceride esters (2,3) of unsaturated fatty acids...

  11. SP (4,R) symmetry in light nuclei

    International Nuclear Information System (INIS)

    Peterson, D.R.

    1979-01-01

    A classification of nuclear states according to the noncompact sympletic Lie algebras sp(2n,R), n = 1, 2, 3, is investigated. Such a classification has recently been shown to be physically meaningful. This classification scheme is the appropriate generalization fo Elliott's SU 3 model of rotational states in deformed light nuclei to include core excitations. A restricted classification according to the Lie algebra, sp(4,R), is motivated. Truncation of the model space to a single sp(4,R) irreducible representation allows the inclusion of states possessing very high excitation energy. An sp(4,R) model study is performed on S = T = 0 positive-parity rotational bands in the deformed light nuclei 16 O and 24 Mg. States are included in the model space that possess up to 10h ω in excitation energy. Results for the B(E2) transition rates compare favorable with experiment, without resort to effective charges

  12. Changes of plasma SS, SP contents in adult patients with primary hypothyroidism

    International Nuclear Information System (INIS)

    Zheng Xianghong; Song Changyi; Lei Yamei; Ning Ning; Chen Wei; Li Runming

    2008-01-01

    Objective: To investigate the possible mechanism of imparirment of central nervous system function in hypothyroid patients through determination of changes of plasma neuropeptides after thyroid hormone replacement therapy. Methods: Plasma somatostatin (SS) and substance P(SP) contents were measured with RIA in 45 patients with primary hypothyroidism both before and after thyroid hormone replacement therapy as well as in 38 controls. Results: Before treatment, the plasma contents of SP in the patients were significantly lower than those in the controls (P 0.05). However, the plasma contents of SS in the more advanced hypothyroid patients with FT 3 3 , FT 4 levels, the plasma SS, SP increased significantly (vs before treatment P<0.05, P<0.01). Conclusion: The decrease of plasma contents of SS and SP in patients with hypothyroidism might be related to the development of psycho-neurological symptoms in these patients and thyroid hormone replacement therapy was desirable. (authors)

  13. An algorithm and a Tool for Wavelength Allocation in OMS-SP Ring Architecture

    DEFF Research Database (Denmark)

    Riaz, Muhammad Tahir; Pedersen, Jens Myrup; Madsen, Ole Brun

    2006-01-01

    OMS-SP ring is one of the well known architectures in Wavelength Division Multiplexing based optical fiber networks. The architecture supports a restorable full mesh in an optical fiber ring using multiple light wavelengths. The paper presents an algorithm to allocate wavelengths in the OMS-SP ri...... architecture. A tool is also introduced which implements the algorithm and assigns wavelengths. The proposed algorithm uses fewer number of wavelengths than the classical allocation method. The algorithm is described and results are presented.......OMS-SP ring is one of the well known architectures in Wavelength Division Multiplexing based optical fiber networks. The architecture supports a restorable full mesh in an optical fiber ring using multiple light wavelengths. The paper presents an algorithm to allocate wavelengths in the OMS-SP ring...

  14. AÇÃO ANTIFÚNGICA in vitro DE ISOLADOS DE Bacillu s sp. SOBRE Fusarium oxysporum f. sp. lycopersici

    Directory of Open Access Journals (Sweden)

    ODENILSON DE DEUS RIBEIRO LIMA

    2014-01-01

    Full Text Available This study aimed to evaluate antagonism and metabolites produced by different species of Ba- cillus in the inhibition of mycelial growth in vitro against F. oxysporum f. sp . lycopersici . For evaluating the antagonism of Bacillus spp. F. oxysporum f. sp . lycopersici was performed pairing of fungus and bacteria by the method of the circle. In the method for detection for the quality for thermostable metabolites liquids. Media BD were used for growth of the isolated Bacillus sp. And incubated for 15 days. After this period, was added 3 g of agar in each flask, and autoclaved broth and poured into Petri dishes. In the center of the plates were placed discs culture of the pathogen. The experimental design was completely randomized with 11 treatments and six repetitions in both experiments. Statistical difference was found between the isolate and the control. Special mention to strains B12 ( Bacillus sp., B41 ( B. cereus , B22' ( B.pentothenticus , B45 ( B. cereus , B47 ( B. cereus that exhibited the lowest average diameter of the colony. To study the inhibition of mycelial growth of F. oxysporum f. sp. lycopersici by thermostatable metabolites five differ statistically from the control they are: B35 ( B. pumilus , B47 ( B. cereus , B22' ( B. pentothenticus , B12 ( Bacillus sp. and B41 ( B. cereus the latter two treatments showed the best results of the pathogen colony diameters and 3.81 to 2.89 cm, respective- ly. B12 and B41 Isolates showed that their antibiotic products were able to inhibit 67.88 % and 57,66 % of F. oxysporum f. sp. lycopersici . These results highlight the possibility of using isolates of the genus Bacillus in the fight against fusarium wilt in tomato.

  15. Cellulose powder from Cladophora sp. algae.

    Science.gov (United States)

    Ek, R; Gustafsson, C; Nutt, A; Iversen, T; Nyström, C

    1998-01-01

    The surface are and crystallinity was measured on a cellulose powder made from Cladophora sp. algae. The algae cellulose powder was found to have a very high surface area (63.4 m2/g, N2 gas adsorption) and build up of cellulose with a high crystallinity (approximately 100%, solid state NMR). The high surface area was confirmed by calculations from atomic force microscope imaging of microfibrils from Cladophora sp. algae.

  16. Novel genetic linkage of rat Sp6 mutation to Amelogenesis imperfecta

    Directory of Open Access Journals (Sweden)

    Muto Taro

    2012-06-01

    Full Text Available Abstract Background Amelogenesis imperfecta (AI is an inherited disorder characterized by abnormal formation of tooth enamel. Although several genes responsible for AI have been reported, not all causative genes for human AI have been identified to date. AMI rat has been reported as an autosomal recessive mutant with hypoplastic AI isolated from a colony of stroke-prone spontaneously hypertensive rat strain, but the causative gene has not yet been clarified. Through a genetic screen, we identified the causative gene of autosomal recessive AI in AMI and analyzed its role in amelogenesis. Methods cDNA sequencing of possible AI-candidate genes so far identified using total RNA of day 6 AMI rat molars identified a novel responsible mutation in specificity protein 6 (Sp6. Genetic linkage analysis was performed between Sp6 and AI phenotype in AMI. To understand a role of SP6 in AI, we generated the transgenic rats harboring Sp6 transgene in AMI (Ami/Ami + Tg. Histological analyses were performed using the thin sections of control rats, AMI, and Ami/Ami + Tg incisors in maxillae, respectively. Results We found the novel genetic linkage between a 2-bp insertional mutation of Sp6 gene and the AI phenotype in AMI rats. The position of mutation was located in the coding region of Sp6, which caused frameshift mutation and disruption of the third zinc finger domain of SP6 with 11 cryptic amino acid residues and a stop codon. Transfection studies showed that the mutant protein can be translated and localized in the nucleus in the same manner as the wild-type SP6 protein. When we introduced the CMV promoter-driven wild-type Sp6 transgene into AMI rats, the SP6 protein was ectopically expressed in the maturation stage of ameloblasts associated with the extended maturation stage and the shortened reduced stage without any other phenotypical changes. Conclusion We propose the addition of Sp6 mutation as a new molecular diagnostic criterion for the

  17. Novel genetic linkage of rat Sp6 mutation to Amelogenesis imperfecta

    Science.gov (United States)

    2012-01-01

    Background Amelogenesis imperfecta (AI) is an inherited disorder characterized by abnormal formation of tooth enamel. Although several genes responsible for AI have been reported, not all causative genes for human AI have been identified to date. AMI rat has been reported as an autosomal recessive mutant with hypoplastic AI isolated from a colony of stroke-prone spontaneously hypertensive rat strain, but the causative gene has not yet been clarified. Through a genetic screen, we identified the causative gene of autosomal recessive AI in AMI and analyzed its role in amelogenesis. Methods cDNA sequencing of possible AI-candidate genes so far identified using total RNA of day 6 AMI rat molars identified a novel responsible mutation in specificity protein 6 (Sp6). Genetic linkage analysis was performed between Sp6 and AI phenotype in AMI. To understand a role of SP6 in AI, we generated the transgenic rats harboring Sp6 transgene in AMI (Ami/Ami + Tg). Histological analyses were performed using the thin sections of control rats, AMI, and Ami/Ami + Tg incisors in maxillae, respectively. Results We found the novel genetic linkage between a 2-bp insertional mutation of Sp6 gene and the AI phenotype in AMI rats. The position of mutation was located in the coding region of Sp6, which caused frameshift mutation and disruption of the third zinc finger domain of SP6 with 11 cryptic amino acid residues and a stop codon. Transfection studies showed that the mutant protein can be translated and localized in the nucleus in the same manner as the wild-type SP6 protein. When we introduced the CMV promoter-driven wild-type Sp6 transgene into AMI rats, the SP6 protein was ectopically expressed in the maturation stage of ameloblasts associated with the extended maturation stage and the shortened reduced stage without any other phenotypical changes. Conclusion We propose the addition of Sp6 mutation as a new molecular diagnostic criterion for the autosomal recessive AI patients

  18. Betulinic acid inhibits colon cancer cell and tumor growth and induces proteasome-dependent and -independent downregulation of specificity proteins (Sp transcription factors

    Directory of Open Access Journals (Sweden)

    Pathi Satya

    2011-08-01

    Full Text Available Abstract Background Betulinic acid (BA inhibits growth of several cancer cell lines and tumors and the effects of BA have been attributed to its mitochondriotoxicity and inhibition of multiple pro-oncogenic factors. Previous studies show that BA induces proteasome-dependent degradation of specificity protein (Sp transcription factors Sp1, Sp3 and Sp4 in prostate cancer cells and this study focused on the mechanism of action of BA in colon cancer cells. Methods The effects of BA on colon cancer cell proliferation and apoptosis and tumor growth in vivo were determined using standardized assays. The effects of BA on Sp proteins and Sp-regulated gene products were analyzed by western blots, and real time PCR was used to determine microRNA-27a (miR-27a and ZBTB10 mRNA expression. Results BA inhibited growth and induced apoptosis in RKO and SW480 colon cancer cells and inhibited tumor growth in athymic nude mice bearing RKO cells as xenograft. BA also decreased expression of Sp1, Sp3 and Sp4 transcription factors which are overexpressed in colon cancer cells and decreased levels of several Sp-regulated genes including survivin, vascular endothelial growth factor, p65 sub-unit of NFκB, epidermal growth factor receptor, cyclin D1, and pituitary tumor transforming gene-1. The mechanism of action of BA was dependent on cell context, since BA induced proteasome-dependent and proteasome-independent downregulation of Sp1, Sp3 and Sp4 in SW480 and RKO cells, respectively. In RKO cells, the mechanism of BA-induced repression of Sp1, Sp3 and Sp4 was due to induction of reactive oxygen species (ROS, ROS-mediated repression of microRNA-27a, and induction of the Sp repressor gene ZBTB10. Conclusions These results suggest that the anticancer activity of BA in colon cancer cells is due, in part, to downregulation of Sp1, Sp3 and Sp4 transcription factors; however, the mechanism of this response is cell context-dependent.

  19. SP-100 from ground demonstration to flight validation

    International Nuclear Information System (INIS)

    Buden, D.

    1989-01-01

    The SP-100 program is in the midst of developing and demonstrating the technology of a liquid-metal-cooled fast reactor using thermoelectric thermal-to-electric conversion devices for space power applications in the range of tens to hundreds of kilowatts. The current ground engineering system (GES) design and development phase will demonstrate the readiness of the technology building blocks and the system to proceed to flight system validation. This phase includes the demonstration of a 2.4-MW(thermal) reactor in the nuclear assembly test (NAT) and aerospace subsystem in the integrated assembly test (IAT). The next phase in the SP-100 development, now being planned, is to be a flight demonstration of the readiness of the technology to be incorporated into future military and civilian missions. This planning will answer questions concerning the logical progression of the GES to the flight validation experiment. Important issues in planning the orderly transition include answering the need to plan for a second reactor ground test, the method to be used to test the SP-100 for acceptance for flight, the need for the IAT prior to the flight-test configuration design, the efficient use of facilities for GES and the flight experiment, and whether the NAT should be modified based on flight experiment planning

  20. Penggunaan Jamur Antagonis Trichoderma SP. Dan Gliocladium SP. Untuk Mengendalikan Penyakit Layu Fusarium Pada Tanaman Bawang Merah (Allium Ascalonicum L.)

    OpenAIRE

    Arie Ramadhina, Arie Ramadhina; Lisnawita, Lisnawita; Lubis, Lahmuddin

    2013-01-01

    The use of antagonism fungus of Trichoderma sp. and Gliocladium sp. for controlling wilt(Fusarium oxysporum) in red onion plants. The aim of the research was to know the effectiviness ofantagonism fungus of Trichoderma sp. and Gliocladium sp. in controlling wilt in red onion plants.The research used non-factorial RAK (random group design) with eight treatments: control, 10grams of F. oxysporum, 12 grams of Trichoderma sp., 18 grams of Trichoderma sp., 24 grams ofTrichoderma sp., and 12 grams ...

  1. Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries-inducing Streptococcus mutans.

    Science.gov (United States)

    Wasfi, Reham; Abd El-Rahman, Ola A; Zafer, Mai M; Ashour, Hossam M

    2018-03-01

    Streptococcus mutans contributes significantly to dental caries, which arises from homoeostasic imbalance between host and microbiota. We hypothesized that Lactobacillus sp. inhibits growth, biofilm formation and gene expression of Streptococcus mutans. Antibacterial (agar diffusion method) and antibiofilm (crystal violet assay) characteristics of probiotic Lactobacillus sp. against Streptococcus mutans (ATCC 25175) were evaluated. We investigated whether Lactobacillus casei (ATCC 393), Lactobacillus reuteri (ATCC 23272), Lactobacillus plantarum (ATCC 14917) or Lactobacillus salivarius (ATCC 11741) inhibit expression of Streptococcus mutans genes involved in biofilm formation, quorum sensing or stress survival using quantitative real-time polymerase chain reaction (qPCR). Growth changes (OD600) in the presence of pH-neutralized, catalase-treated or trypsin-treated Lactobacillus sp. supernatants were assessed to identify roles of organic acids, peroxides and bacteriocin. Susceptibility testing indicated antibacterial (pH-dependent) and antibiofilm activities of Lactobacillus sp. against Streptococcus mutans. Scanning electron microscopy revealed reduction in microcolony formation and exopolysaccharide structural changes. Of the oral normal flora, L. salivarius exhibited the highest antibiofilm and peroxide-dependent antimicrobial activities. All biofilm-forming cells treated with Lactobacillus sp. supernatants showed reduced expression of genes involved in exopolysaccharide production, acid tolerance and quorum sensing. Thus, Lactobacillus sp. can inhibit tooth decay by limiting growth and virulence properties of Streptococcus mutans. © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

  2. SpEnD: Linked Data SPARQL Endpoints Discovery Using Search Engines

    Science.gov (United States)

    Yumusak, Semih; Dogdu, Erdogan; Kodaz, Halife; Kamilaris, Andreas; Vandenbussche, Pierre-Yves

    In this study, a novel metacrawling method is proposed for discovering and monitoring linked data sources on the Web. We implemented the method in a prototype system, named SPARQL Endpoints Discovery (SpEnD). SpEnD starts with a "search keyword" discovery process for finding relevant keywords for the linked data domain and specifically SPARQL endpoints. Then, these search keywords are utilized to find linked data sources via popular search engines (Google, Bing, Yahoo, Yandex). By using this method, most of the currently listed SPARQL endpoints in existing endpoint repositories, as well as a significant number of new SPARQL endpoints, have been discovered. Finally, we have developed a new SPARQL endpoint crawler (SpEC) for crawling and link analysis.

  3. Interaction of uranium with Pleurotus sp

    International Nuclear Information System (INIS)

    Ohnuki, Toshihiko; Sakamoto, Fuminori; Kozaki, Naofumi; Ozaki, Takuro; Samadfam, Mohammad

    2002-01-01

    Uptake of uranium by higher fungi, such as mushroom is little elucidated. We have studied the interaction of uranium with Pleurotus sp. (a mushroom) in pure culture over a wide range of U concentration (50-3000 mg/L). The Pleurotus sp. was cultured in two different media. One was rice bran medium, and the other was agar (yeast extract, peptone and dextrose) medium. The uptake of uranium in Pleurotus sp. was examined by alpha ray autoradiography (A,A), X-ray fluorescence spectroscopy (XRF) and scanning microcopy (SEM) equipped with EDS. In the agar medium, the higher uranium concentration gave lower growth of mycelia, and no fruiting body was observed. In the rice bran medium, the fruiting body was grown at U concentrations up to 1000 mg/L. The AA and XRF analysis showed that uranium taken up in the fruiting body was below the detection limit. The SEM-EDS analysis indicated that U was distributed in the limited region and was not transported to the mycelia far from U containing medium. It is concluded that uranium affects the growth of Pleurotus sp., and little uranium is taken up by Pleurotus sp. during the growth of both mycelia and fruiting body. (author)

  4. IAA-producing Penicillium sp. NICS01 triggers plant growth and suppresses Fusarium sp.-induced oxidative stress in sesame (Sesamum indicum L.).

    Science.gov (United States)

    Radhakrishnan, Ramalingam; Shim, Kang-Bo; Lee, Byeong-Won; Hwang, Chung-Dong; Pae, Suk-Bok; Park, Chang-Hwan; Kim, Sung-Up; Lee, Choon-Ki; Baek, In-Youl

    2013-06-28

    Application of rhizospheric fungi is an effective and environmentally friendly method of improving plant growth and controlling many plant diseases. The current study was aimed to identify phytohormone-producing fungi from soil, to understand their roles in sesame plant growth, and to control Fusarium disease. Three predominant fungi (PNF1, PNF2, and PNF3) isolated from the rhizospheric soil of peanut plants were screened for their growth-promoting efficiency on sesame seedlings. Among these isolates, PNF2 significantly increased the shoot length and fresh weight of seedlings compared with controls. Analysis of the fungal culture filtrate showed a higher concentration of indole acetic acid in PNF2 than in the other isolates. PNF2 was identified as Penicillium sp. on the basis of phylogenetic analysis of ITS sequence similarity. The in vitro biocontrol activity of Penicillium sp. against Fusarium sp. was exhibited by a 49% inhibition of mycelial growth in a dual culture bioassay and by hyphal injuries as observed by scanning electron microscopy. In addition, greenhouse experiments revealed that Fusarium inhibited growth in sesame plants by damaging lipid membranes and reducing protein content. Co-cultivation with Penicillium sp. mitigated Fusarium-induced oxidative stress in sesame plants by limiting membrane lipid peroxidation, and by increasing the protein concentration, levels of antioxidants such as total polyphenols, and peroxidase and polyphenoloxidase activities. Thus, our findings suggest that Penicillium sp. is a potent plant growthpromoting fungus that has the ability to ameliorate damage caused by Fusarium infection in sesame cultivation.

  5. Métodos de cultura de algas do plancton marinho: estudos realizados nas regiões de Cananéia e de Ubatuba, SP Culture methods of planktonic marine alrae: studies in Cananéia and Ubatuba regions

    Directory of Open Access Journals (Sweden)

    Armando A. H Vieira

    1977-12-01

    Full Text Available The study of algae culture collected in Brazilian waters was proposed due to the lack of literature on the subject: an attempt was made to develop a methodology to obtain algae culture and its purification. Several species were isolated from samples collected a Ubatuba and Cananéia (São Paulo (Lat. 23º30'S - Long. 45º06'W and Lat. 25º01'S - Long. 47º54'W by means of phytoplankton net and Van Dorn bottle. The following isolation techniques were employed after washing and concentration: glass capillary, inoculation of mixed cultures on Petri dishes with solid medium and positive phototropism. The isolated algae were kept in five different mediuns. Washing techniques were tested (centrifugation, "filter tubes" and re-isolation , antibiotics and ultra violet radiation to obtain unialgal bacteria-free cultures were employed to obtain pure cultures. Five différents antibiotics, mixed in différents concentrations, were employed during several periods of expositions. The re-isolation proved to be the best method to obtain the purification of the cultures.

  6. Taxonomy Icon Data: Synechocystis sp.PCC 6803 [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available Synechocystis sp.PCC 6803 Synechocystis sp.PCC 6803 Synechocystis_sp_PCC_6803_L.png Synecho...cystis_sp_PCC_6803_NL.png Synechocystis_sp_PCC_6803_S.png Synechocystis_sp_PCC_6803_NS.png http://bi...osciencedbc.jp/taxonomy_icon/icon.cgi?i=Synechocystis+sp%2ePCC+6803&t=L http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Synecho...cystis+sp%2ePCC+6803&t=NL http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Synecho...cystis+sp%2ePCC+6803&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Synechocystis

  7. Five novel Wickerhamomyces- and Metschnikowia-related yeast species, Wickerhamomyces chaumierensis sp. nov., Candida pseudoflosculorum sp. nov., Candida danieliae sp. nov., Candida robnettiae sp. nov. and Candida eppingiae sp. nov., isolated from plants

    NARCIS (Netherlands)

    Groenewald, Marizeth; Robert, Vincent; Smith, Maudy Th

    On the basis of nucleotide divergences in the D1/D2 domain of the 26S rRNA gene and the internal transcribed spacers (ITS) domain of the rRNA gene, five novel yeast species, Wickerhamomyces chaumierensis sp. nov. (CBS 8565(T)  = JCM 17246(T)), Candida pseudoflosculorum sp. nov. (CBS 8584(T)  = JCM

  8. [Investigation of Cryptosporidium sp. in workers of the Van municipality slaughterhouse and in slaughtered animals].

    Science.gov (United States)

    Ciçek, Mutalip; Körkoca, Hanifi; Gül, Abdurrahman

    2008-01-01

    This study was carried out in order to investigate the prevalence of Cryptosporidium sp. in slaughtered animals and workers of the Van municipality slaughterhouse in Van. Animals slaughtered at different times and workers who had been working in different departments of the slaughter house were included in the study for three months. A total of 309 fecal specimens from animals including 167 sheep, 56 goats and 86 cattle and 87 fecal specimens from workers were examined for Cryptosporidium sp. oocysts. In slaughtered animals, the modified acid-fast staining method was used to determine the oocysts of Cryptosporidium sp. The fecal samples of slaughter workers were examined by using RIDA (R) Quick Cryptosporidium Strip Test (R-Biopharm, Germany) and the modified acid-fast staining method. Fecal samples found to be positive by stripe test were also confirmed with the ELISA method (R-Biopharm, Germany). Oocysts of Cryptosporidium sp. were found in fecal specimens of 22 sheep (13.17%), 6 goats (10.71%) and 7 cattle (8.13%). Intestinal parasites were observed in 34 fecal specimens of workers (39.08%). Cryptosporidium sp., Hymenolepis nana, Chilomastix mesnili, Endolimax nana, Iodamoeba bütschlii were found in the specimen of one worker (1.14%), Entamoeba coli in 4 workers (4.59%), Blastocystis hominis (9.19%) in 8 workers, and Giardia intestinalis (19.54%) in 17 workers.

  9. Growth and Cultivation of the Unusual Generalized Transducing Bacillus Bacteriophage SP-15

    Science.gov (United States)

    Taylor, Martha J.; Goldberg, Ivan D.

    1971-01-01

    Additional properties of SP-15, a generalized transducing bacteriophage notable for the ability to transfer an unusually large fragment of deoxyribonucleic acid (DNA) to Bacillus subtilis and B. licheniformis, are presented together with improved methods that enhance its utility. Simple means have been found to provide the rigid control over moisture that is necessary for the assay of plaque-forming units (PFU). Reproducible procedures for propagating transducing phage, which depend upon an appropriate mixing of PFU with uninfected bacteria, have replaced less reliable methods that utilized infected spores. Transduction of B. subtilis W-23 increased linearly when MgSO4 in recipient cell-SP-15 mixtures was increased from 0.005 to 0.03 m. Methods have been developed that protect SP-15 from the damaging effects of CsCl and of osmotic shock subsequent to dilution. Evidence that the PFU and transducing particles of lysates decay at the same slow rate during extended storage suggests that the decay is a result of damage to protein rather than to DNA. One-step growth experiments, in which SP-15 was propagated on B. subtilis W-23-Sr/1 mg, indicated a latent period of 100 min, a rise period of 60 min, and a burst size of 25 to 34 PFU per infected cell. These findings suggest explanations for some of the technical difficulties SP-15 has presented. PMID:4999971

  10. SP2Bench: A SPARQL Performance Benchmark

    Science.gov (United States)

    Schmidt, Michael; Hornung, Thomas; Meier, Michael; Pinkel, Christoph; Lausen, Georg

    A meaningful analysis and comparison of both existing storage schemes for RDF data and evaluation approaches for SPARQL queries necessitates a comprehensive and universal benchmark platform. We present SP2Bench, a publicly available, language-specific performance benchmark for the SPARQL query language. SP2Bench is settled in the DBLP scenario and comprises a data generator for creating arbitrarily large DBLP-like documents and a set of carefully designed benchmark queries. The generated documents mirror vital key characteristics and social-world distributions encountered in the original DBLP data set, while the queries implement meaningful requests on top of this data, covering a variety of SPARQL operator constellations and RDF access patterns. In this chapter, we discuss requirements and desiderata for SPARQL benchmarks and present the SP2Bench framework, including its data generator, benchmark queries and performance metrics.

  11. The gamma ray spectrometer GA.SP

    Energy Technology Data Exchange (ETDEWEB)

    Bazzacco, D [Instituto Nazionale di Fisica Nucleare, Sezione di Padova, Padova (Italy)

    1992-08-01

    GA.SP is a general purpose 4{pi} detector array for advanced {gamma}-spectroscopy and, in the same time, a suitable system for reaction mechanism studies. The detector is sited at the LNL Tandem+Linac accelerator and has been built as a joint project of INFN Padova, LNL, Milano and Firenze. The array consists of 40 Compton suppressed HPGe detectors and of a 4{pi} calorimeter composed of 80 BGO crystals. The detector houses a reaction chamber of 34 cm diameter where a charged particles multiplicity filter composed of 40 Si detectors is going to be installed. Evaporation residues produced in the centre of GA.SP can be injected into the recoil mass spectrometer (RMS, named CAMEL) in use at LNL, without the need to remove any of the gamma detectors. The coupled operation of GA.SP, RMS and Si ball will give a unique instrument for identification and study of weak reaction channels. (author). 6 figs.

  12. The gamma ray spectrometer GA.SP

    International Nuclear Information System (INIS)

    Bazzacco, D.

    1992-01-01

    GA.SP is a general purpose 4π detector array for advanced γ-spectroscopy and, in the same time, a suitable system for reaction mechanism studies. The detector is sited at the LNL Tandem+Linac accelerator and has been built as a joint project of INFN Padova, LNL, Milano and Firenze. The array consists of 40 Compton suppressed HPGe detectors and of a 4π calorimeter composed of 80 BGO crystals. The detector houses a reaction chamber of 34 cm diameter where a charged particles multiplicity filter composed of 40 Si detectors is going to be installed. Evaporation residues produced in the centre of GA.SP can be injected into the recoil mass spectrometer (RMS, named CAMEL) in use at LNL, without the need to remove any of the gamma detectors. The coupled operation of GA.SP, RMS and Si ball will give a unique instrument for identification and study of weak reaction channels. (author). 6 figs

  13. SP-100/Brayton power system concepts

    International Nuclear Information System (INIS)

    Owen, D.F.

    1989-01-01

    Use of closed Brayton cycle (CBC) power conversion technology has been investigated for use with SP-100 reactors for space power systems. The CBC power conversion technology is being developed by Rockwell International under the Dynamic Isotype Power System (DIPS) and Space Station Freedom solar dynamic power system programs to provide highly efficient power conversion with radioisotype and solar collector heat sources. Characteristics including mass, radiator area, thermal power, and operating temperatures for systems utilizing SP-100 reactor and CBC power conversion technology were determined for systems in the 10-to 100-kWe power range. Possible SP-100 reactor/CBC power system configurations are presented. Advantages of CBC power conversion technology with regard to reactor thermal power, operating temperature, and development status are discussed

  14. Radioiodinate labeling of atherosclerotic plaque imaging agent SP-4 and preliminary experiments

    International Nuclear Information System (INIS)

    Zhang, Y.; Wu, Z.

    2000-01-01

    SP-4 was oligopeptide contained 18 amino-acid. It was a part of apolipoprotein B. To study labeling SP-4 with 131 I and its clinical prospect as an atherosclerotic plaque imaging agent. SP-4 was synthesized by solid phase method and identified by amino acid analysis after purification with preparation-model HPLC. SP-4 was labeled with 131 I by the Chloramine-T method and purified through Sephadex G-25, then the radiochemical purity of 131 SP-4 and its stability in vitro were analyzed. 12 New Zealand rabbits were divided into atherosclerosis group (n=7, group A) and control group (n=5, group B). All of them were administrated with bovine serum albumen through i.v., then the rabbits of group A were fed on high cholesterol and high fat diet and group B, on normal diet. Purified 131 I-SP-4 was injected intravenously. %ID/g in blood and thoracic aorta and abdominal aorta at 4 hrs after injection and biodistribution of 131 I-SP-4 was investigated. The amino acid formation of the pure product was identified to be correct through amino-acid analysis. The radiochemical purity of 131 I-SP-4 was 96.2% after being purified, but less than 90% after being stored for 20 hrs. One of 7 rabbits in group A died after being fed for three weeks, the others were alive and atherosclerotic lesions were found after being fed for two mon. On the contrary, 5 rabbits in group B were visualized not to have atherosclerotic lesions. The uptakes of group A and group B at 4 hr after injection were 0.0378±0.0028 and 0.0371±0.038 in blood (p>0.05), 0.0882 ±0.0101 and 0.0276 ±0.0044 in abdominal aorta (p 131 I-SP-4 was mainly excreted through kidneys. SP-4 remained its biological activity after radioiodination and was located at atherosclerotic lesions. It was potentially useful as an atherosclerotic plaque imaging agent

  15. *sp131*I-3-iodobenzylguanidine (*sp131*I-3-IBG) as a scintigraphic agent for the visualization of adrenal medulla tumors

    International Nuclear Information System (INIS)

    Heggeli, D.E.; Brorson, B.I.; Bremper, P.O.

    1983-06-01

    A method of labelling 3-iodobenzylguanidine with *sp131*I is described. 3-IBG . 0.5 H*sb2*SO*sb4* and Cu(II)SO*sb4* were dissolved in a 0.1 M NH*sb4*H*sb2*PO*sb4* buffer and mixed with *sp131*I-NaI. The solution was evaporated to dryness by heating. After addition of water, the solution was heated with reflux for two hours. The I*sp-* ions were removed after labelling by anionic exchange chromatography. The final product was made isotonic and bacteriostatic by the addition of acetate buffer, saline and benzylalcohol. The product was filtered through a membrane filter with a pore size of 0.22*my*m and was apyrogenically tested by limulus test. The tumors of adrenal medulla, pheochromocytomas and neuroplastomas may in some cases be small or located extra-adrenally. In those cases *sp131*I-labelled 3-IBG is a valuable tool, since 3-IBG concentrates in adrenal medulla tumors because ot its analogy to the catecholamines. Injecting a dose of 0.5 mCi *sp131*I-3-IBG (2.5 mCi/mg), which is an adult dose, allows the scintigraphic localization of the tumours, thus guiding the surgeon. Adrenal uptake in mice and dog is described in the report, as well as a rapid method for the control of radiochemical purity. The radioactive concentration of the *sp131*I-3-IBG has been found to be important for the radiochemical stability of the product. (RF)

  16. Discovery of New Eunicellins from an Indonesian Octocoral Cladiella sp.

    Science.gov (United States)

    Chen, Yung-Husan; Tai, Chia-Ying; Su, Yin-Di; Chang, Yu-Chia; Lu, Mei-Chin; Weng, Ching-Feng; Su, Jui-Hsin; Hwang, Tsong-Long; Wu, Yang-Chang; Sung, Ping-Jyun

    2011-01-01

    Two new 11-hydroxyeunicellin diterpenoids, cladieunicellin F (1) and (–)-solenopodin C (2), were isolated from an Indonesian octocoral Cladiella sp. The structures of eunicellins 1 and 2 were established by spectroscopic methods, and eunicellin 2 was found to be an enantiomer of the known eunicellin solenopodin C (3). Eunicellin 2 displayed inhibitory effects on the generation of superoxide anion and the release of elastase by human neutrophils. The previously reported structures of two eunicellin-based compounds, cladielloides A and B, are corrected in this study. PMID:21747739

  17. Modifications of Sp(2) covariant superfield quantization

    Energy Technology Data Exchange (ETDEWEB)

    Gitman, D.M.; Moshin, P.Yu

    2003-12-04

    We propose a modification of the Sp(2) covariant superfield quantization to realize a superalgebra of generating operators isomorphic to the massless limit of the corresponding superalgebra of the osp(1,2) covariant formalism. The modified scheme ensures the compatibility of the superalgebra of generating operators with extended BRST symmetry without imposing restrictions eliminating superfield components from the quantum action. The formalism coincides with the Sp(2) covariant superfield scheme and with the massless limit of the osp(1,2) covariant quantization in particular cases of gauge-fixing and solutions of the quantum master equations.

  18. SP-100 converter multicouple thermoelectric cell

    International Nuclear Information System (INIS)

    Kull, R.A.; Terrill, W.R.

    1990-01-01

    The General Electric Company is under contract to DOE to design, fabricate, and test an SP-100 Ground Engineering System. This paper provides a description of the SP-100 space reactor power system configuration, and a more detailed description of the power conversion subsystem (PCSS) and the key building block of the power converter, the thermoelectric cell. The functions of the various elements of the PCSS and the cells are also presented. These cells convert the thermal energy from the reactor into electrical power at the desired voltage while being conductively coupled to the hot and cold side heat exchangers to maximize the power output and system specific power

  19. Radioiodine labelled SP-4 as an imaging agent for atherosclerotic plaques

    International Nuclear Information System (INIS)

    Zhang Yongxue; Wu Zhijian; Cao Wei

    2000-01-01

    The clinical prospect of radioiodinated SP-4 as an atherosclerotic plaque imaging agent was studied. The SP-4 was synthesized by a solid phase method and identified by an amino acid analysis after purification with HPLC. SP-4 was labelled with 131 I and 125 I by the Chloramine-T method and purified through Sephadex G-25 column. Twelve New Zealand rabbits were divided into an atherosclerotic group (n = 7, AR) and a control group (n = 5, NR). All of the atherosclerotic rabbits were intravenous administrated with bovine serum albumin, then feb with high cholesterol and fat diet. 125 I-SP-4 was intravenous administrated to the rabbits of both groups. The biodistribution of 125 I-SP-4 in rabbits was investigated. The uptakes (% ID/g) in blood and thoracic aorta and abdominal aorta were calculated 4 hours postinjection. Macro-autoradiography and micro-autoradiography were performed in 2 AR atherosclerotic abdominal aortas. The clearance of radioactivity from plasma was very rapid. 125 I-SP-4 was mainly excreted through kidneys. The radioactive uptakes of abdominal aorta and thoracic aorta of AR at 4 hours postinjection were significantly higher than that of NR. The films of macro-autoradiography showed focal accumulation of the radioactivity in the areas of a newly formed edges of atherosclerotic plaques. On the slices of micro-autoradiography, the obvious radioactive accumulation could be found in the atherosclerotic plaques. Thus it was seen that the SP-4 remained its biological activity after radioiodination and was located at atherosclerotic lesions, it is potentially useful as an atherosclerotic plaque imaging agent

  20. Halobacterium sp. SP1(1) as a starter culture for accelerating fish sauce fermentation.

    Science.gov (United States)

    Akolkar, A V; Durai, D; Desai, A J

    2010-07-01

    Application of Halobacterium sp. SP1(1) for the acceleration of fish sauce fermentation. Traditional fish sauce fermentation was mimicked using Halobacterium sp. SP1(1) as starter culture. Protease activity, peptide release and α-amino content (parameters used to monitor the progress of the fermentation) were high at day 10 in tests and day 20 in un-inoculated controls. The total protein and nitrogen contents were also high in tests compared with controls. The amino acid profile observed at the end of fermentation in experimental samples, when compared with the commercial sauce preparation, was found to be better with respect to flavour and aroma contributing amino acids as well as essential amino acid lysine. Microflora analysis of the final fish sauce revealed the absence of any nonhalophilic or halotolerant micro-organisms. The protease-producing halophilic isolates obtained from the fish sauce of eviscerated and uneviscerated controls were identified as Halobacterium sp. F1 and F2, respectively, by 16S rDNA sequence analysis. Exogenous augmentation of Halobacterium sp. SP1(1) accelerated the fish sauce fermentation process with an additive effect on the existing natural microflora present in the fish during fermentation. Halobacterium sp SP1(1), therefore, can be used as an important starter culture for accelerating the fish fermentation process, which is attributed to its extracellular protease. The present study is the first report on use of Halobacterium species as a starter culture for accelerating fish sauce fermentation. Use of halobacterial starter cultures may revolutionize the process in fish sauce industries by reducing the fermentation time and making the process more economical with improved nutritive value of product. Journal compilation © 2009 The Society for Applied Microbiology. No claim to Indian Government works.

  1. 76 FR 56876 - Proposed Collection; Comment Request for Forms 9779, 9779(SP), 9783, 9783(SP), 9787, 9787(SP...

    Science.gov (United States)

    2011-09-14

    ... and 9789(SP), Electronic Federal Tax Payment System (EFTPS). DATES: Written comments should be received on or before November 14, 2011 to be assured of consideration. ADDRESSES: Direct all [email protected] . SUPPLEMENTARY INFORMATION: Title: Electronic Federal Tax Payment System (EFTPS). OMB...

  2. Study of Shallow Low-Enthalpy Geothermal Resources Using Integrated Geophysical Methods

    Science.gov (United States)

    De Giorgi, Lara; Leucci, Giovanni

    2015-02-01

    The paper is focused on low enthalpy geothermal exploration performed in south Italy and provides an integrated presentation of geological, hydrogeological, and geophysical surveys carried out in the area of municipality of Lecce. Geological and hydrogeological models were performed using the stratigraphical data from 51 wells. A ground-water flow (direction and velocity) model was obtained. Using the same wells data, the ground-water annual temperature was modeled. Furthermore, the ground surface temperature records from ten meteorological stations were studied. This allowed us to obtain a model related to the variations of the temperature at different depths in the subsoil. Integrated geophysical surveys were carried out in order to explore the low-enthalpy geothermal fluids and to evaluate the results of the model. Electrical resistivity tomography (ERT) and self-potential (SP) methods were used. The results obtained upon integrating the geophysical data with the models show a low-enthalpy geothermal resource constituted by a shallow ground-water system.

  3. Common genetic variants of surfactant protein-D (SP-D are associated with type 2 diabetes.

    Directory of Open Access Journals (Sweden)

    Neus Pueyo

    Full Text Available CONTEXT: Surfactant protein-D (SP-D is a primordial component of the innate immune system intrinsically linked to metabolic pathways. We aimed to study the association of single nucleotide polymorphisms (SNPs affecting SP-D with insulin resistance and type 2 diabetes (T2D. RESEARCH DESIGN AND METHODS: We evaluated a common genetic variant located in the SP-D coding region (rs721917, Met(31Thr in a sample of T2D patients and non-diabetic controls (n = 2,711. In a subset of subjects (n = 1,062, this SNP was analyzed in association with circulating SP-D concentrations, insulin resistance, and T2D. This SNP and others were also screened in the publicly available Genome Wide Association (GWA database of the Meta-Analyses of Glucose and Insulin-related traits Consortium (MAGIC. RESULTS: We found the significant association of rs721917 with circulating SP-D, parameters of insulin resistance and T2D. Indeed, G carriers showed decreased circulating SP-D (p = 0.004, decreased fasting glucose (p = 0.0002, glycated hemoglobin (p = 0.0005, and 33% (p = 0.002 lower prevalence of T2D, estimated under a dominant model, especially among women. Interestingly, these differences remained significant after controlling for origin, age, gender, and circulating SP-D. Moreover, this SNP and others within the SP-D genomic region (i.e. rs10887344 were significantly associated with quantitative measures of glucose homeostasis, insulin sensitivity, and T2D, according to GWAS datasets from MAGIC. CONCLUSIONS: SP-D gene polymorphisms are associated with insulin resistance and T2D. These associations are independent of circulating SP-D concentrations.

  4. Quantitation of pulmonary surfactant protein SP-B in the absence or presence of phospholipids by enzyme-linked immunosorbent assay

    DEFF Research Database (Denmark)

    Oviedo, J M; Valiño, F; Plasencia, I

    2001-01-01

    We have developed an enzyme-linked immunosorbent assay (ELISA) that uses polyclonal or monoclonal anti-surfactant protein SP-B antibodies to quantitate purified SP-B in chloroform/methanol and in chloroform/methanol extracts of whole pulmonary surfactant at nanogram levels. This method has been...... used to explore the effect of the presence of different phospholipids on the immunoreactivity of SP-B. Both polyclonal and monoclonal antibodies produced reproducible ELISA calibration curves for methanolic SP-B solutions with protein concentrations in the range of 20-1000 ng/mL. At these protein...

  5. SP-A binding sites on bovine alveolar macrophages.

    Science.gov (United States)

    Plaga, S; Plattner, H; Schlepper-Schaefer, J

    1998-11-25

    Surfactant protein A (SP-A) binding to bovine alveolar macrophages was examined in order to characterize SP-A binding proteins on the cell surface and to isolate putative receptors from these cells that could be obtained in large amounts. Human SP-A, unlabeled or labeled with gold particles, was bound to freshly isolated macrophages and analyzed with ELISA or the transmission electron microscope. Binding of SP-A was inhibited by Ca2+ chelation, by an excess of unlabeled SP-A, or by the presence of 20 mg/ml mannan. We conclude that bovine alveolar macrophages expose binding sites for SP-A that are specific and that depend on Ca2+ and on mannose residues. For isolation of SP-A receptors with homologous SP-A as ligand we isolated SP-A from bovine lung lavage. SDS-PAGE analysis of the purified SP-A showed a protein of 32-36 kDa. Functional integrity of the protein was demonstrated. Bovine SP-A bound to Dynabeads was used to isolate SP-A binding proteins. From the fractionated and blotted proteins of the receptor preparation two proteins bound SP-A in a Ca2+-dependent manner, a 40-kDa protein showing mannose dependency and a 210-kDa protein, showing no mannose sensitivity. Copyright 1998 Academic Press.

  6. Effect of probiotic microorganisms isolated from Hypostomus plecostomus in Oreochromis sp juveniles

    Directory of Open Access Journals (Sweden)

    Yuri Vela-Gutiérrez

    2017-01-01

    Full Text Available Objective. The aim of this study was to assess the behavior of red tilapia juveniles (Oreochromis sp. in presence of the possible probiotic microorganisms isolated from the gastrointestinal tract of panche fish (Hypostomus plecostomus. Materials and methods. During the phase of assessment of probiotics, 120 red tilapia (Oreochromis sp juveniles were selected, with average weight of 1.28±0.22 g and average length of 2.99±0.27 cm. Three experimental treatments were formulated taking into account the nourishment needs of red tilapia (Oreochromis sp.. The statistical method employed was a completely random design. Results. The three strains of Lactobacillus genus (Lactobacillus plantarum, Lactobacillus pentosus, Lactobacillus rhamnosus isolated from the digestive tract of H. plecostomus, yielded diverse results in the biometrical variables considered, as well as in the SGR, PER, AE, AC. Conclusions. The best rapports regarding the gain in length were for the treatment with native probiotics, and it is deduced that they were easily settled in the intestines of the fish (Oreochromis sp.. It is also determined that there are significant differences in the results or gain in length for the Control Concentrate feed (CC against Concentrate feed plus Native Probiotic (CNP for red tilapia (Oreochromis sp. in the juvenile stag for a 30 day time span.

  7. Safely Intake Number of Macridiscus sp. (Kerang Ceplos) from Tambak Lorok Waters, Semarang, Central Java, Indonesia

    Science.gov (United States)

    Meirenno Tielman, Eduard; Suprijanto, Jusup; Widowati, Ita

    2018-02-01

    The dynamics pollution that supposed to be derived from industrial activities around Tambak Lorok waters will affect the quality of waters, and also biota such as Macridiscus sp. mussels (Kerang Ceplos) that live and accumulate pollutants such as heavy metals (Pb, Cu, Al, Mn and Fe). However, Macridiscus sp. mussels which have been contaminated by heavy metals is usually sold for consumption by the people and if they consume it in excess, it will be toxic in the people’s body. So that, this study was to analyze Safely Intake Number of Macridiscus sp. from Tambak Lorok waters. This study used AAS (Atomic Absorption Spectrophotometry) method to analyze the accumulation number of the pollutant (Pb, Cu, Al, Mn and Fe). Safely Intake Number calculation is used MWI (Maximal Weekly Intake) and MTI (Maximal Tolerable Intake) calculation method. The results of AAS showed that the highest numbers of pollutant was Al (reached 534,51 mg/kg in the body of Macridiscus sp. that taken in February, 2016) and has exceeded the safely intake number (MWI Al = 1 mg/kg, based on WHO/FAO) so that it’s MTI values was low (0,08 kg/week/person). It means that Macridiscus sp. was not safe to be consumed excessively at that time.

  8. Gilliamella intestini sp. nov., Gilliamella bombicola sp. nov., Gilliamella bombi sp. nov. and Gilliamella mensalis sp. nov.: Four novel Gilliamella species isolated from the bumblebee gut.

    Science.gov (United States)

    Praet, Jessy; Cnockaert, Margo; Meeus, Ivan; Smagghe, Guy; Vandamme, Peter

    2017-06-01

    Spectra of five isolates (LMG 28358 T , LMG 29879 T , LMG 29880 T , LMG 28359 T and R-53705) obtained from gut samples of wild bumblebees of Bombus pascuorum, Bombus lapidarius and Bombus terrestris were grouped into four MALDI-TOF MS clusters. RAPD analysis revealed an identical DNA fingerprint for LMG 28359 T and R-53705 which also grouped in the same MALDI-TOF MS cluster, while different DNA fingerprints were obtained for the other isolates. Comparative 16S rRNA gene sequence analysis of the four different strains identified Gilliamella apicola NCIMB 14804 T as nearest neighbour species. Average nucleotide identity values of draft genome sequences of the four isolates and of G. apicola NCIMB 14804 T were below the 96% threshold value for species delineation and all four strains and G. apicola NCIMB 14804 T were phenotypically distinct. Together, the draft genome sequences and phylogenetic and phenotypic data indicate that the four strains represent four novel Gilliamella species for which we propose the names Gilliamella intestini sp. nov., with LMG 28358 T as the type strain, Gilliamella bombicola sp. nov., with LMG 28359 T as the type strain, Gilliamella bombi sp. nov., with LMG 29879 T as the type strain and Gilliamella mensalis sp. nov., with LMG 29880 T as the type strain. Copyright © 2017 Elsevier GmbH. All rights reserved.

  9. Biosorption of uranium by Azolla, SP, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Ludmila C.; Alves, Eliakim G.; Marumo, Julio T., E-mail: lcvieira@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Ferreira, Rafael V. de P., E-mail: rafael@itatijuca.com [Itatijuca Biotech, Sao Paulo, SP (Brazil); Canevesi, Rafael L.S.; Silva, Edson A., E-mail: edson.silva2@unioeste.br [Universidade Estadual do Oeste Parana (UNIOESTE), Toledo, PR (Brazil)

    2015-07-01

    Radioactive liquid waste needs special attention and requires suitable treatment before deposition. Among the potential technologies under development for the treatment of liquid radioactive wastes the biosorption has been highlighted by being an efficient and low cost technique. Biosorption process involves the exchange of ions contained in the biomass matrix by others present in solution. There are many biomasses that could be applied in treatment of radioactive wastes, for example, agricultural residues and macrophyte. The aim of this study is evaluate the ability of the Azolla sp., a floating aquatic plant, to absorb uranium in solution. Azolla sp. is a macrophyte that has been used to treat effluents containing heavy metals. The biosorption capacity of uranium by Azolla sp. was experimentally determined and modeled by isotherms. Experiments were performed to determine metal uptake, and then the solutions were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES). The isotherms applied to model the data was Langmuir, Freundlich, Sips Toth, Redlich Peternson, Two-Site-Langmuir, Radke Prausnitz to develop a technique for the treatment of radioactive liquid waste generated at the Nuclear and Energy Research Institute (IPEN-CNEN/SP), Brazil. (author)

  10. Biosorption of uranium by Azolla, SP, Brazil

    International Nuclear Information System (INIS)

    Vieira, Ludmila C.; Alves, Eliakim G.; Marumo, Julio T.; Ferreira, Rafael V. de P.; Canevesi, Rafael L.S.; Silva, Edson A.

    2015-01-01

    Radioactive liquid waste needs special attention and requires suitable treatment before deposition. Among the potential technologies under development for the treatment of liquid radioactive wastes the biosorption has been highlighted by being an efficient and low cost technique. Biosorption process involves the exchange of ions contained in the biomass matrix by others present in solution. There are many biomasses that could be applied in treatment of radioactive wastes, for example, agricultural residues and macrophyte. The aim of this study is evaluate the ability of the Azolla sp., a floating aquatic plant, to absorb uranium in solution. Azolla sp. is a macrophyte that has been used to treat effluents containing heavy metals. The biosorption capacity of uranium by Azolla sp. was experimentally determined and modeled by isotherms. Experiments were performed to determine metal uptake, and then the solutions were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES). The isotherms applied to model the data was Langmuir, Freundlich, Sips Toth, Redlich Peternson, Two-Site-Langmuir, Radke Prausnitz to develop a technique for the treatment of radioactive liquid waste generated at the Nuclear and Energy Research Institute (IPEN-CNEN/SP), Brazil. (author)

  11. Carbon sp chains in graphene nanoholes

    DEFF Research Database (Denmark)

    Castelli, Ivano Eligio; Ferri, Nicola; Onida, Giovanni

    2012-01-01

    Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. ...

  12. Rhodotorula bloemfonteinensis sp. nov., Rhodotorula eucalyptica sp. nov., Rhodotorula orientis sp. nov. and Rhodotorula pini sp. nov., yeasts isolated from monoterpene-rich environments.

    Science.gov (United States)

    Pohl, Carolina H; Smit, Martha S; Albertyn, Jacobus

    2011-09-01

    Recent rDNA sequencing of 25 isolates from a previous study, during which limonene-utilizing yeasts were isolated from monoterpene-rich environments by using 1,4-disubstituted cyclohexanes as sole carbon sources, led to the identification of four hitherto unknown Rhodotorula species. Analyses of the 26S rDNA D1/D2 region as well as the internal transcribed spacer (ITS) domain indicated that two isolates (CBS 8499(T) and CBS 10736) were identical and were closely related to Rhodotorula cycloclastica, a previously described limonene-utilizing yeast. These novel isolates differed from known yeast species and could be distinguished from R. cycloclastica by standard physiological tests. The other three isolates represent three novel Rhodotorula species, closely related to Sporobolomyces magnisporus. These three species could also be distinguished from other Rhodotorula species by standard physiological tests. Based on these results, we suggest that the new isolates represent novel species, for which the names Rhodotorula eucalyptica sp. nov. (type strain CBS 8499(T)  = NRRL Y-48408(T)), Rhodotorula pini sp. nov. (type strain CBS 10735(T)  = NRRL Y-48410(T)), Rhodotorula bloemfonteinensis sp. nov. (type strain CBS 8598(T)  = NRRL Y-48407(T)) and Rhodotorula orientis sp. nov. (type strain CBS 8594(T)  = NRRL Y-48719(T)) are proposed. R. eucalyptica and R. pini can also utilize limonene.

  13. Test af spørgeskema

    DEFF Research Database (Denmark)

    Hermansen, Jonathan

    2017-01-01

    Denne bog gennemgår alle surveyarbejdets faser og giver en praktisk indføring i •design af undersøgelsen og udvælgelse af stikprøver, •formulering af spørgeskemaer samt indsamling og kodning af data, •metoder til at analysere resultaterne....

  14. Lipid contents of the sponge Haliclona sp.

    Digital Repository Service at National Institute of Oceanography (India)

    Parameswaran, P.S.; Das, B.; Kamat, S.Y.

    Several fatty acids, sterols, batyl alcohol and its analogs and an N-acylated sphingosine (ceramide) have been isolated from the lipid fraction of the extract of the sponge Haliclona sp. The major sterol is found to be cholesterol (54%), followed...

  15. Optisk scanning af spørgeskemaer

    DEFF Research Database (Denmark)

    Paulsen, Aksel

    / 1000 felter= 0.370 (95% CI: 0.160-0.729), (p= 0.020)). Der var ingen statistisk forskel mellem optisk scanning (fejl/ 1000 felter= 0.046 (95% CI: 0.001-0.258)) og manuel dobbelt indtastning (p=1.000). Konklusioner: Optisk scanning er et godt alternativ til manuel dobbelt indtastning for spørgeskema med......Formål og baggrund: Patient rapporterede outcomes i form af spørgeskemaer bruges i øgende grad i sundhedssektoren både i klinisk praksis og forskning. Ofte bruges spørgeskemaer i papirformat. Manuel dobbelt indtastning er defineret som guld standard for overføring af data til et elektronisk format......, men processen er tidkrævende. Optisk scanning af spørgeskemaer med automatisk registrering af svar kan være et alternativ, men videre validering af metoden er nødvendig. Design: 200 patienter blev tilfældigt udvalgt fra en kohorte på 5777 patienter der tidligere havde svaret på to forskellige...

  16. Aerococcus sp. with an antimycobacterial effect

    African Journals Online (AJOL)

    Yomi

    2011-12-21

    Dec 21, 2011 ... In this study, we reported data on a strain that was isolated from different areas of Fez. (Morocco), which ... sp. The antibacterial activity of the genus Aerococcus have .... able to amplify the 16S rRNA gene from eubacteria.

  17. Botrallin from the endophytic fungus Hyalodendriella sp ...

    African Journals Online (AJOL)

    use

    2011-12-12

    Dec 12, 2011 ... Bioassay-guided fractionation of the crude methanol extract of the mycelia from the endophytic fungus. Hyalodendriella sp. Ponipodef12, associated with the hybrid 'Neva' of Populus deltoides Marsh × P. nigra L., led to the isolation of one compound coded as P12-1 which was identified as botrallin (1,7-.

  18. The astaxanthin dideoxyglycoside biosynthesis pathway in Sphingomonas sp. PB304

    DEFF Research Database (Denmark)

    Kim, Se Hyeuk; Kim, Jin Ho; Lee, Bun Yeol

    2014-01-01

    A major carotenoid in Sphingomonas sp. PB304, originally isolated from a river in Daejon City, South Korea, was identified as astaxanthin dideoxyglycoside. Gene clusters encoding the astaxanthin dideoxyglycoside biosynthetic enzymes were identified by screening Sphingomonas sp. PB304 fosmid...

  19. SP-100 initial startup and restart control strategy

    Science.gov (United States)

    Halfen, Frank J.; Wong, Kwok K.; Switick, Dennis M.; Shukla, Jaikaran N.

    Startup control strategies for SP-100 are described. Revised control and operating strategies are discussed which have been developed and tested using the SP-100 dynamic simulation model Aries-GFS (Generic Flight System).

  20. Storage Methods Of Plantain ( Musa sp. AAB) Fruits Influenced ...

    African Journals Online (AJOL)

    The results showed that about 67% of fruits stored in plane, yellow and red Po were green after two weeks as against only 8% of fruits lined on LS. The inclusion of sawdust/salt hastened senescence and enhanced disease development. Severity of crown rot was generally lowest in blue polyethylene. Also, fruits stored in ...

  1. Experimental measurements of the SP response to concentration and temperature gradients in sandstones with application to subsurface geophysical monitoring

    Science.gov (United States)

    Leinov, E.; Jackson, M. D.

    2014-09-01

    Exclusion-diffusion potentials arising from temperature gradients are widely neglected in self-potential (SP) surveys, despite the ubiquitous presence of temperature gradients in subsurface settings such as volcanoes and hot springs, geothermal fields, and oil reservoirs during production via water or steam injection. Likewise, with the exception of borehole SP logging, exclusion-diffusion potentials arising from concentration gradients are also neglected or, at best, it is assumed that the diffusion potential dominates. To better interpret these SP sources requires well-constrained measurements of the various coupling terms. We report measurements of thermoelectric and electrochemical exclusion-diffusion potentials across sandstones saturated with NaCl brine and find that electrode effects can dominate the measured voltage. After correcting for these, we find that Hittorf transport numbers are the same within experimental error regardless of whether ion transport occurs in response to temperature or concentration gradients over the range of NaCl concentration investigated that is typical of natural systems. Diffusion potentials dominate only if the pore throat radius is more than approximately 4000 times larger than the diffuse layer thickness. In fine-grained sandstones with small pore throat diameter, this condition is likely to be met only if the saturating brine is of relatively high salinity; thus, in many cases of interest to earth scientists, exclusion-diffusion potentials will comprise significant contributions from both ionic diffusion through, and ionic exclusion from, the pore space of the rock. However, in coarse-grained sandstones, or sandstones saturated with high-salinity brine, exclusion-diffusion potentials can be described using end-member models in which ionic exclusion is neglected. Exclusion-diffusion potentials in sandstones depend upon pore size and salinity in a complex way: they may be positive, negative, or zero depending upon sandstone

  2. Streptomycetes antagonism against Cladosporium fulvum Cooke and Fusarium oxysporium f.sp. lycopersici Antagonismo de estreptomicetos a Cladosporium fulvum Cooke e Fusarium oxysporium f.sp. lycopersici

    Directory of Open Access Journals (Sweden)

    Ana Cristina Fermino Soares

    2009-09-01

    Full Text Available This research aimed to evaluate the secondary effects of secondary metabolites produced by streptomycetes on spore germination and mycelial growth of the phytopathogenic fungi Cladosporium fulvum Cooke and Fusarium oxysporium f. sp. lycopersici from tomato plants. Metabolites produced by streptomycete isolates codified as AC-147 and AC-92 caused 94.1% inhibition of C. fulvum while AC-95 isolate caused 33.9% inhibition. AC-92 was the most efficient for F. oxysporum f. sp. lycopersici, causing 94.2% inhibition of spore germination. For mycelial growth, AC-26 and AC-92 were the most efficient in inhibiting C. fulvum growth by 46.6% and F. oxysporum f. sp. lycopersici by 29.9%. These streptomycetes are potential agents for biocontrol development methods of these tomato plant pathogenic fungi.Este trabalho teve como objetivo avaliar o efeito de metabólitos secundários produzidos por estreptomicetos na germinação de esporos e no crescimento micelial dos fungos Cladosporium fulvum Cooke e Fusarium oxysporum sp. f. lycopersici da cultura do tomateiro. Metabólitos produzidos pelos isolados AC-147 e AC-92 causaram 94,1% de inibição da germinação de esporos de C. fluvum, enquanto que o isolado AC-95 causou 33,9% de inibição. O AC-92 foi o mais eficiente para F. oxysporum f. sp. lycopersici, causando 94,2% de inibição na germinação de esporos. Para o crescimento micelial, AC-26 e AC-92 foram os mais eficientes na inibição dos fungos C. fulvum, em 46,6%, e F. oxysporum f. sp. Lycopersici, em 29,9%. Esses estreptomicetos são potenciais agentes para o desenvolvimento de métodos de controle biológico desses fungos fitopatogênicos do tomateiro.

  3. One-step production of biodiesel from Nannochloropsis sp. on solid base Mg-Zr catalyst

    International Nuclear Information System (INIS)

    Li, Yuesong; Lian, Shuang; Tong, Dongmei; Song, Ruili; Yang, Wenyan; Fan, Yong; Qing, Renwei; Hu, Changwei

    2011-01-01

    Nannochloropsis sp., one kind of green microalgae cultivated autotrophically and axenically in laboratory, is used as raw material to produce biodiesel by one-step method in an amended reactor. The effects of several reaction parameters on transesterification over Mg-Zr solid base catalyst were investigated through both conventional method and one-step method. One-step method could give a higher yield of methyl ester than conventional two-step method, which demonstrates that the present one-step method is suitable for biodiesel production from the microalgae Nannochloropsis sp. Moreover, the present one-step method realizes the convenient in situ separation of catalyst from microalgae residue which can be easily used consequently, reducing the procedure units as well as the overall costs.

  4. Alternate hosts of spongospora subterranea f. sp. subterranea identification in colombia by bioassay / identificación de hospederos alternos de spongospora subterranea f. sp. subterranea en colombia por bioensayos

    OpenAIRE

    Arcila Aristizabal, Ivon Magaly; González Jaimes, Elena Paola; Zuluaga Amaya, Catalina María; Cotes Torres, José Miguel

    2013-01-01

    Potato powdery scab, caused by Spongosporasubterranea f. sp. subterranea, is a disease that limits worldwide potato crop production. Incidence of the disease has been increasing in Colombia, thereby affecting tubers production, so far effective control methods have yet to be developed. The aim of this research was to establish the host range plants for Spongospora subterranea f. sp. subterranea by artificial inoculations. Thus, 33 species were inoculated with 1×106 sporosori.mL-1 solution for...

  5. Methylobacterium suomiense sp. nov. and Methylobacterium lusitanum sp. nov., aerobic, pink-pigmented, facultatively methylotrophic bacteria.

    Science.gov (United States)

    Doronina, Nina V; Trotsenko, Yuri A; Kuznetsov, Boris B; Tourova, Tatjana P; Salkinoja-Salonen, Mirja S

    2002-05-01

    Two aerobic, pink-pigmented, facultatively methylotrophic bacteria, strains F20T and RXM(T), are described taxonomically. On the basis of their phenotypic and genotypic properties, the isolates are proposed as novel species of the genus Methylobacterium, Methylobacterium suomiense sp. nov. (type strain F20T = VKM B-2238T = NCIMB 13778T) and Methylobacterium lusitanum sp. nov. (type strain RXMT = VKM B-2239T = NCIMB 13779T).

  6. Nanocrystalline sp{sup 2} and sp{sup 3} carbons: CVD synthesis and applications

    Energy Technology Data Exchange (ETDEWEB)

    Terranova, M. L. [Università degli Studi di Roma “Tor Vergata,” via Della Ricerca Scientifica, Dipartimento di Scienze e Tecnologie Chimiche—MinimaLab (Italy); Rossi, M. [Università degli Studi di Roma “Sapienza,” via A. Scarpa, Dipartimento di Scienze di Base e Applicate per l’Ingegneria and Centro di Ricerca per le Nanotecnologie Applicate all’Ingegneria (CNIS) (Italy); Tamburri, E., E-mail: emanuela.tamburri@uniroma2.it [Università degli Studi di Roma “Tor Vergata,” via Della Ricerca Scientifica, Dipartimento di Scienze e Tecnologie Chimiche—MinimaLab (Italy)

    2016-11-15

    The design and production of innovative materials based on nanocrystalline sp{sup 2}- and sp{sup 3}-coordinated carbons is presently a focus of the scientific community. We present a review of the nanostructures obtained in our labs using a series of synthetic routes, which make use of chemical vapor deposition (CVD) techniques for the selective production of non-planar graphitic nanostructures, nanocrystalline diamonds, and hybrid two-phase nanostructures.

  7. Evaluación de la estabilidad de Trichoderma sp. y Azotobacter sp. conservados por diferentes métodos

    Directory of Open Access Journals (Sweden)

    Yvonne Sarmiento Rangel

    2013-01-01

    Full Text Available Título en ingles: Evaluation of the stability of Trichoderma sp. and Azotobacter sp.  preserved by different methods Resumen En el Laboratorio de Microbiología Aplicada de la Universidad Francisco de Paula Santander (Cúcuta, Colombia constantemente ingresan al Banco de Cepas, cultivos microbianos de interés biotecnológico, especialmente del sector agrícola, los cuales son utilizados en las actividades de docencia e investigación.  Por esta razón, existe el interés de mantener viables a través del tiempo estos cultivos microbianos, para lo cual se realizó en esta investigación, la evaluación de la estabilidad de los aislados de Trichoderma sp. y Azotobacter sp., utilizando  las técnicas de conservación  en viales con solución salina estéril (0,85% NaCl en refrigeración (4°C y temperatura ambiente (30°C, suelo estéril en refrigeración (4°C, comparados con la metodología de repiques sucesivos como tratamiento control.  Los resultados no mostraron diferencias significativas según el Test de Duncan (P≤0,05 en  la tasa de supervivencia microbiana entre los métodos de conservación. Sin embargo, se observó en las técnicas de viales con solución salina  estéril y suelo estéril mantenidos en refrigeración, mayor estabilidad en la concentración celular durante los cuatros meses de evaluación, sin registrar contaminación en los cultivos.  Así mismo, se registró un óptimo crecimiento macroscópico de los cultivos microbianos y sus características microscópicas se mantuvieron estables en estos dos métodos de conservación.  Por esta razón, se seleccionaron como técnicas de conservación de estos microorganismos, teniendo en cuenta además, ventajas como la facilidad de la técnica, disponibilidad de equipos, materiales y personal con los que se cuenta en el laboratorio.  Palabras clave: banco de cepas, conservación de microorganismos, viabilidad celular, Trichoderma sp., Azotobacter sp. Abstract In the

  8. Differences in nutrient uptake capacity of the benthic filamentous algae Cladophora sp., Klebsormidium sp. and Pseudanabaena sp. under varying N/P conditions.

    Science.gov (United States)

    Liu, Junzhuo; Vyverman, Wim

    2015-03-01

    The N/P ratio of wastewater can vary greatly and directly affect algal growth and nutrient removal process. Three benthic filamentous algae species Cladophora sp., Klebsormidium sp. and Pseudanabaena sp. were isolated from a periphyton bioreactor and cultured under laboratory conditions on varying N/P ratios to determine their ability to remove nitrate and phosphorus. The N/P ratio significantly influenced the algal growth and phosphorus uptake process. Appropriate N/P ratios for nitrogen and phosphorus removal were 5-15, 7-10 and 7-20 for Cladophora sp., Klebsormidium sp. and Pseudanabaena sp., respectively. Within these respective ranges, Cladophora sp. had the highest biomass production, while Pseudanabaena sp. had the highest nitrogen and phosphorus contents. This study indicated that Cladophora sp. had a high capacity of removing phosphorus from wastewaters of low N/P ratio, and Pseudanabaena sp. was highly suitable for removing nitrogen from wastewaters with high N/P ratio. Copyright © 2014 Elsevier Ltd. All rights reserved.

  9. Biosorption of chromium by mangrove-derived Aplanochytrium sp ...

    African Journals Online (AJOL)

    The microbial dried biomass of Thraustochytrids is used as bioadsorbent for the removal of the chromium in aqueous solution. In this investigation, three species of Thraustochydrids namely Aplanochytrium sp., Thraustochytrium sp. and Schizochytrium sp. were tested for the efficiency of chromium accumulation by culturing ...

  10. Babesia peircei sp. nov. from the jackass penguin

    African Journals Online (AJOL)

    1992-01-09

    Jan 9, 1992 ... An avian piroplasm, Babesia peircei sp. nov. is described from the jackass penguin Spheniscus demersus. Morphological differences between Babesia peircei sp. nov. and the other valid Babesia spp. are discussed together with the possible vectors. 'n Voal-piroplasma, Babesia peircei sp. nov. afkomstig ...

  11. Environmental conditions affecting exopolysaccharide production by Pseudomonas aeruginosa, Micrococcus sp., and Ochrobactrum sp.

    Science.gov (United States)

    Kiliç, Nur Koçberber; Dönmez, Gönül

    2008-06-15

    Three different chromium-resistant microorganisms (Pseudomonas aeruginosa, Micrococcus sp., and Ochrobactrum sp.) were tested with regard to their EPS production at different pH levels, temperatures, Cr(VI) concentrations, and incubation periods. The optimum pH level was 7 for P. aeruginosa and Micrococcus sp., while it was 8 for Ochrobactrum sp. according to the highest EPS amount at 100 mg/L Cr(VI) concentration. The highest production of EPSs by the three bacteria was obtained under different environmental conditions. P. aeruginosa produced the highest EPS (863.3 mg/L) after incubation for 96 h on media with 50 mg/L Cr(VI) at 20 degrees C, Micrococcus sp. gave the highest yield (444.6 mg/L) after incubation for 72 h on media with 100 mg/L Cr(VI) at the same temperature, and Ochrobactrum sp. had the highest production (430.5 mg/L) on media with 150 mg/L Cr(VI) at 30 degrees C at the end of 48 h of incubation.

  12. Remote sensing data of SP Mountain and SP Lava flow in North-Central Arizona

    Science.gov (United States)

    Schaber, G.G.; Elachi, C.; Farr, T.G.

    1980-01-01

    Multifrequency airborne radar image data of SP Mountain [Official name of feature (U.S. Geological Survey, 1970)] and SP flow (and vicinity) in north-central Arizona were obtained in diverse viewing directions and direct and cross-polarization, then compared with surface and aerial photography, LANDSAT multispectral scanner data, airborne thermal infrared imagery, surface geology, and surface roughness statistics. The extremely blocky, basaltic andesite of SP flow is significantly brighter on direct-polarization K-band (0.9-cm wavelength) images than on cross-polarized images taken simultaneously. Conversely, for the longer wavelength (25 cm) L-band radar images, the cross-polarization image returns from SP flow are brighter than the direct-polarized image. This effect is explained by multiple scattering and the strong wavelength dependence of polarization effects caused by the rectilinear basaltic andesite scatters. Two distinct types of surface relief on SP flow, one extremely blocky, the other subdued, are found to be clearly discriminated on the visible and thermal wavelength images but are separable only on the longer wavelength L-band radar image data. The inability of the K- and X- (3-cm wavelength) band radars to portray the differences in roughness between the two SP flow surface units is attributed to the radar frequency dependence of the surface-relief scale, which, described as the Rayleigh criterion, represents the transition between quasispecular and primarily diffuse backscatter. ?? 1980.

  13. Two new species of suctorians, Acineta satyanandani sp. nov. and Paracineta karunakarani sp. nov. epizoic on ostracods

    Digital Repository Service at National Institute of Oceanography (India)

    Santhakumari, V.

    Two, species of protozoic suctorians, Acineta satyanandani sp. nov. and Paracineta karunakarani sp. nov., are described. These were found attached on the body of the marine ostracod, Cypridina dentata (Muller), collected from the shelf and slope...

  14. Biogeographical Variation and Population Genetic Structure of Sporisorium scitamineum in Mainland China: Insights from ISSR and SP-SRAP Markers

    Directory of Open Access Journals (Sweden)

    Liping Xu

    2014-01-01

    Full Text Available A total of 100 Sporisorium scitamineum isolates were investigated by inter simple sequence repeat (ISSR and single primer-sequence related amplified polymorphism (SP-SRAP markers. These isolates were clearly assorted into three distinct clusters regardless of method used: either cluster analysis or by principal component analysis (PCA of the ISSR, SP-SRAP, or ISSR + SP-SRAP data set. The total gene diversity (Ht and gene diversity between subpopulations (Hs were estimated to be 0.34 to 0.38 and 0.22 to 0.29, respectively, by analyzing separately the ISSR and SP-SRAP data sets, and to be 0.26–0.36 by analyzing ISSR + SP-SRAP data set. The gene diversity attributable to differentiation among populations (Gst was estimated to be 0.35 and 0.22, and the gene flow (Nm was 0.94 and 1.78, respectively, when analyzing separately ISSR and SP-SRAP data set, and was 0.27 and 1.33, respectively, when analyzing ISSR + SP-SRAP data set. Our study showed that there is considerable genetic variation in the analyzed 100 isolates, and the environmental heterogeneity has played an important role for this observed high degree of variation. The genetic differentiation of sugarcane smut fungus depends to a large extent on the heterogeneity of their habitats and is the result of long-term adaptations of pathogens to their ecological environments.

  15. Microbiologically influenced corrosion of galvanized steel by Desulfovibrio sp. and Desulfosporosinus sp. in the presence of Ag–Cu ions

    Energy Technology Data Exchange (ETDEWEB)

    Ilhan-Sungur, Esra, E-mail: esungur@istanbul.edu.tr [Istanbul University, Faculty of Science, Department of Biology, 34134 Vezneciler, Istanbul (Turkey); Unsal-Istek, Tuba [Istanbul University, Faculty of Science, Department of Biology, 34134 Vezneciler, Istanbul (Turkey); Cansever, Nurhan [Yıldız Technical University, Faculty of Chemistry-Metallurgy, Metallurgical and Materials Engineering Department, 34210 Esenler, Istanbul (Turkey)

    2015-07-15

    The effects of Ag–Cu ions on the microbiologically induced corrosion of galvanized steel in the presence of Desulfovibrio sp. and Desulfosporosinus sp. were investigated. The corrosion behavior of galvanized steel was analyzed by potentiodynamic polarization and electrochemical impedance spectroscopy. The biofilm, corrosion products and Ag–Cu ions on the surfaces were investigated by using scanning electron microscopy, energy dispersive X-ray spectrometry and elemental mapping. The biofilm layer formed by the Desulfovibrio sp. was stable covering the all surface of galvanized steel coupons, while that by Desulfosporosinus sp. was intermittent, highly porous and heterogeneous. It was found that both of the sulfate reducing bacteria species accelerated corrosion of the galvanized steel. However, it was detected that Desulfosporosinus sp. was more corrosive for galvanized steel than Desulfovibrio sp. Scanning electron microscopy images showed that Desulfovibrio sp. and Desulfosporosinus sp. in biofilm clustered into patches on the galvanized steel surface when the culture contained toxic Ag–Cu ions. The ions affected the growth of the sulfate reducing bacteria strains in different ways and hence the corrosion behaviors. It was observed that the Ag–Cu ions affected negatively growth of Desulfosporosinus sp. especially after 24 h of exposure leading to a decrease in the corrosion rate of galvanized steel. However, Desulfovibrio sp. showed more corrosive effect in the presence of the ions according to the ions-free culture. Energy dispersive X-ray spectrometry analysis showed that corrosion products on the surfaces were mainly composed of Zn, S, Na, O and P. - Highlights: • Galvanized steel was corroded by Desulfosporosinus sp. and Desulfovibrio sp. • Desulfosporosinus sp. is more corrosive than Desulfovibrio sp. • The Ag–Cu ions affected corrosion behavior of Desulfosporosinus sp. and Desulfovibrio sp. on galvanized steel.

  16. In vitro cytotoxicity evaluation of nano-carbon particles with different sp{sup 2}/sp{sup 3} ratios

    Energy Technology Data Exchange (ETDEWEB)

    Li, S.S.; Wu, B.J.; Deng, Q.Y. [Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031 (China); Guo, Y.B. [The Third People' s Hospital of Chengdu, Sichuan 610031 (China); Leng, Y.X., E-mail: yxleng@263.net [Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031 (China); Huang, N. [Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031 (China)

    2017-06-01

    Graphitization occurs during the long-term service of a diamond-like carbon (DLC) modified artificial joint. Then, DLC wear debris, which are carbon particles with different sp{sup 2}/sp{sup 3} ratios and sizes ranging from the nano- to micro-meter scale produced. In this paper, to promote the application of DLC coating for artificial joint modification, the cytotoxicity of DLC debris (nano-carbon particles, NCs) with different sp{sup 2}/sp{sup 3} ratios was studied. The microstructure and physical characteristics of NCs with different sp{sup 2}/sp{sup 3} ratios were investigated by Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), Transmission Electron Microscope (TEM) and Dynamic Light Scattering (DLS). Meanwhile, osteoblasts and macrophages were applied to characterize the cytotoxicity of the NCs. In vitro cytotoxicity assay results indicated that cells incubated with NCs of different sp{sup 2}/sp{sup 3} ratios had greater osteogenic capacity, and these particles caused a weaker immune response in comparison with CoCrMo particles. Taken together, the results indicated that NCs with different sp{sup 2}/sp{sup 3} ratios presented a good cytocompatibility than CoCrMo particles. But no significant differences were observed among NCs with different sp{sup 2}/sp{sup 3} ratios. The better cytocompatibility of NCs is mainly attributable to their surface charge. - Highlights: • NCs with different sp{sup 2}/sp{sup 3} ratios have been successfully prepared by annealing treatment. • NCs with different sp{sup 2}/sp{sup 3} ratios show good osteogenic capacity and lower immune response. • The good cytocompatibility of NCs is mainly dependent on its surface charge.

  17. Microbiologically influenced corrosion of galvanized steel by Desulfovibrio sp. and Desulfosporosinus sp. in the presence of Ag–Cu ions

    International Nuclear Information System (INIS)

    Ilhan-Sungur, Esra; Unsal-Istek, Tuba; Cansever, Nurhan

    2015-01-01

    The effects of Ag–Cu ions on the microbiologically induced corrosion of galvanized steel in the presence of Desulfovibrio sp. and Desulfosporosinus sp. were investigated. The corrosion behavior of galvanized steel was analyzed by potentiodynamic polarization and electrochemical impedance spectroscopy. The biofilm, corrosion products and Ag–Cu ions on the surfaces were investigated by using scanning electron microscopy, energy dispersive X-ray spectrometry and elemental mapping. The biofilm layer formed by the Desulfovibrio sp. was stable covering the all surface of galvanized steel coupons, while that by Desulfosporosinus sp. was intermittent, highly porous and heterogeneous. It was found that both of the sulfate reducing bacteria species accelerated corrosion of the galvanized steel. However, it was detected that Desulfosporosinus sp. was more corrosive for galvanized steel than Desulfovibrio sp. Scanning electron microscopy images showed that Desulfovibrio sp. and Desulfosporosinus sp. in biofilm clustered into patches on the galvanized steel surface when the culture contained toxic Ag–Cu ions. The ions affected the growth of the sulfate reducing bacteria strains in different ways and hence the corrosion behaviors. It was observed that the Ag–Cu ions affected negatively growth of Desulfosporosinus sp. especially after 24 h of exposure leading to a decrease in the corrosion rate of galvanized steel. However, Desulfovibrio sp. showed more corrosive effect in the presence of the ions according to the ions-free culture. Energy dispersive X-ray spectrometry analysis showed that corrosion products on the surfaces were mainly composed of Zn, S, Na, O and P. - Highlights: • Galvanized steel was corroded by Desulfosporosinus sp. and Desulfovibrio sp. • Desulfosporosinus sp. is more corrosive than Desulfovibrio sp. • The Ag–Cu ions affected corrosion behavior of Desulfosporosinus sp. and Desulfovibrio sp. on galvanized steel

  18. Extraction fatty acid as a source to produce biofuel in microalgae Chlorella sp. and Spirulina sp. using supercritical carbon dioxide

    Science.gov (United States)

    Tai, Do Chiem; Hai, Dam Thi Thanh; Vinh, Nguyen Hanh; Phung, Le Thi Kim

    2016-06-01

    In this research, the fatty acids of isolated microalgae were extracted by some technologies such as maceration, Soxhlet, ultrasonic-assisted extraction and supercritical fluid extraction; and analyzed for biodiesel production using GC-MS. This work deals with the extraction of microalgae oil from dry biomass by using supercritical fluid extraction method. A complete study at laboratory of the influence of some parameters on the extraction kinetics and yields and on the composition of the oil in terms of lipid classes and profiles is proposed. Two types of microalgae were studied: Chlorella sp. and Spirulina sp. For the extraction of oil from microalgae, supercritical CO2 (SC-CO2) is regarded with interest, being safer than n-hexane and offering a negligible environmental impact, a short extraction time and a high-quality final product. Whilst some experimental papers are available on the supercritical fluid extraction (SFE) of oil from microalgae, only limited information exists on the kinetics of the process. These results demonstrate that supercritical CO2 extraction is an efficient method for the complete recovery of the neutral lipid phase.

  19. Sp1-mediated transcription regulation of TAF-Ialpha gene encoding a histone chaperone.

    Science.gov (United States)

    Asaka, Masamitsu N; Murano, Kensaku; Nagata, Kyosuke

    2008-11-28

    TAF-I, one of histone chaperones, consists of two subtypes, TAF-Ialpha and TAF-Ibeta. The histone chaperone activity of TAF-I is regulated by dimer patterns of these subtypes. TAF-Ibeta is expressed ubiquitously, while the expression level of TAF-Ialpha with less activity than TAF-Ibeta differs among cell types. It is, therefore, assumed that the expression level of TAF-Ialpha in a cell is important for the TAF-I activity level. Here, we found that TAF-Ialpha and TAF-Ibeta genes are under the control of distinct promoters. Reporter assays and gel shift assays demonstrated that Sp1 binds to three regions in the TAF-Ialpha promoter and two or all mutaions of the three Sp1 binding regions reduced the TAF-Ialpha promoter activity. ChIP assays demonstrated that Sp1 binds to the TAF-Ialpha promoter in vivo. Furthermore, the expression level of TAF-Ialpha mRNA was reduced by knockdown of Sp1 using siRNA method. These studies indicated that the TAF-Ialpha promoter is under the control of Sp1.

  20. INHIBITION OF PATHOGENS BY SPOROGENIC BACTERIA ISOLATED FROM HONEY OF Melipona sp. (APIDAE: APINAE: MELIPONINI

    Directory of Open Access Journals (Sweden)

    KELY DAMIANA NOVAES DA SILVA

    2016-01-01

    Full Text Available The aim of this study was to isolate sporogenic bacteria from the honey of stingless bees Melipona sp., in dry forest, and to evaluate their antagonistic potential for medicinal employment purposes and animal production. The honey samples were collected in Serra Talhada - PE, where honey was taken from four different hives (in triplicate, totaling 12 samples. The samples were diluted and subjected to 80 ºC for 20 minutes to eliminate vegetative cells. The dilutions were plated onto nutrient agar and incubated at 30 ºC for 72 hours. Then the colony forming units (CFU were quantified. The samples were also plated onto malt agar and Sabouraud agar, and incubated at 30 ºC for 14 days for the growth of yeast and molds. Total and fecal coliforms were quantified by the most probable number method (MPN. Seven isolates (I of sporogenic bacteria ( Bacillus were obtained, however only four showed probiotic potential. Isolate I - 5 showed the greatest probiotic potential and inhibited the growth of Escherichia coli , Klebsiella sp., Pseudomonas aeruginosa, Salmonella sp., and Staphylococcus aureus . The growth of the Sarcina sp. was not inhibited by any isolate. No yeast, molds or coliforms were found. The Melipona sp. honey is a source of spore - forming bacteria and is antagonistic to microorganisms that contaminate honey. It has good microbiological quality.

  1. Identifikasi Molekuler Fitoplasma yang Berasosiasi dengan Tanaman Kaktus Hias Opuntia sp.

    Directory of Open Access Journals (Sweden)

    Ariny Prasetya

    2017-11-01

    Full Text Available Cactus species (Opuntia sp. is a popular ornamental succulent plant. Some ornamental cactus species in Indonesia showing proliferation and green mosaic pattern symptoms have been reported to be associated with phytoplasma infection. However, further molecular identification for accurate classification of the causal phytoplasma has not been done.  This study aimed to diagnose phytoplasma associated with Opuntia sp. based on molecular methods involving PCR standard combined with nested-PCR, cloning and DNA sequencing. Standard PCR was carried out using P1/P7 primers followed by nested-PCR using R16F2n/R16R2 or fU5/rU3 primer pairs which amplify the 16S rRNA gene targets of 1.2 kb and 880 bp, respectively. Amplified fragment of nested-PCR using R16F2n/R16R2 primers was chosen to be cloned and sequenced for further identification and classification of phytoplasma.  BLASTn analysis showed that the phytoplasma from Opuntia sp. was closely related to 16SrII group. Phylogenetic analysis and in silico RFLP indicated that phytoplasma strain infecting Opuntia sp. was a member of subgroup 16SrII-C (cactus witches’ broom phytoplasma. This is a newly report of cactus witches’ broom phytoplasma on Opuntia sp. in Indonesia.   

  2. Interaction of Sp1 zinc finger with transport factor in the nuclear localization of transcription factor Sp1

    International Nuclear Information System (INIS)

    Ito, Tatsuo; Kitamura, Haruka; Uwatoko, Chisana; Azumano, Makiko; Itoh, Kohji; Kuwahara, Jun

    2010-01-01

    Research highlights: → Sp1 zinc fingers themselves interact with importin α. → Sp1 zinc finger domains play an essential role as a nuclear localization signal. → Sp1 can be transported into the nucleus in an importin-dependent manner. -- Abstract: Transcription factor Sp1 is localized in the nucleus and regulates the expression of many cellular genes, but the nuclear transport mechanism of Sp1 is not well understood. In this study, we revealed that GST-fused Sp1 protein bound to endogenous importin α in HeLa cells via the Sp1 zinc finger domains, which comprise the DNA binding domain of Sp1. It was found that the Sp1 zinc finger domains directly interacted with a wide range of importin α including the armadillo (arm) repeat domain and the C-terminal acidic domain. Furthermore, it turned out that all three zinc fingers of Sp1 are essential for binding to importin α. Taken together, these results suggest that the Sp1 zinc finger domains play an essential role as a NLS and Sp1 can be transported into the nucleus in an importin-dependent manner even though it possesses no classical NLSs.

  3. Apoptosis of human tongue squamous cell carcinoma cell (CAL-27 induced by Lactobacillus sp. A-2 metabolites

    Directory of Open Access Journals (Sweden)

    Guoliang ZHANG

    2014-07-01

    Full Text Available Objective: To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells. Methods: Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2 were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL and assayed by methyl thiazolyltetrazolium (MTT method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao fluorescent staining; flow cytometry method (FCM and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2. Results: The different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent. Conclusions: Viability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.

  4. Selective C(sp3)−H aerobic oxidation enabled by decatungstate photocatalysis in flow

    NARCIS (Netherlands)

    Laudadio, G.; Govaerts, S.; Wang, Y.; Ravelli, D.; Koolman, H.; Fagnoni, M.; Djuric, S.; Noel, T.

    2018-01-01

    A mild and selective C(sp3)−H aerobic oxidation enabled by decatungstate photocatalysis has been developed. The reaction can be significantly improved in a microflow reactor enabling the safe use of oxygen and enhanced irradiation of the reaction mixture. Our method allows for the oxidation of both

  5. New SP-values of time and reliability for freight transport in the Netherlands

    NARCIS (Netherlands)

    Jong, G. de; Kouwenhoven, M.; Bates, J.; Koster, P.; Verhoef, E.; Tavasszy, L.; Warffemius, P.

    2014-01-01

    This paper discusses the methods used in a study on the values of time and reliability in freight transport in the Netherlands. SP surveys were carried out among more than 800 shippers and carriers. A novel feature is that both for the value of time and reliability two additive components are

  6. Comparison of virulence between vascular competent and incompetent Fusarium oxysporum f. sp. vasinfectum pathotypes

    Science.gov (United States)

    The Australian biotype and California race 4 isolates of Fusarium oxysporum f. sp. Vasinfectum (Fov) are pathologically distinct from the Fov U.S. race 1 isolates in that they do not cause disease when stem-puncture inoculated while race 1 isolates do. When root-dip inoculation method was used, bot...

  7. Hirsutane Sesquiterpenes from Cultures of the Basidiomycete Marasmiellus sp. BCC 22389

    Directory of Open Access Journals (Sweden)

    Masahiko Isaka

    2016-08-01

    Full Text Available Abstract Two new hirsutane sesquiterpenes, marasmiellins A (1 and B (2, were isolated from cultures of the basidiomycete Marasmiellus sp. BCC 22389. The structures were elucidated on the basis of NMR spectroscopic and mass spectrometry data. The absolute configuration of marasmiellin B was determined by application of the modified Mosher’s method. Graphical Abstract

  8. SUPPRESSION ABILITY OF CRUDE EXTRACT DERIVED FROM MARINE BIOTA AGAINST FUSARIUM OXYSPORUM F.SP. VANILLAE

    Directory of Open Access Journals (Sweden)

    I Ketut Suada

    2010-06-01

    Full Text Available The objective of this research was to investigate suppression ability of marine biota extracts against Fusarium oxysporum f.sp. vanillae of vanilla stem rot. Samples were collected at intertidal zones and in the depth of 1-7 m from seven beaches in Bali. Screening of active compounds of biota extracts were conducted using inhibition zone of well diffusion method on Potato Dextrose Agar (PDA. The extract was tested in-vitro in PDA medium using completely randomized design with three replicates. The methanolic extract of Aglaophenia sp. was able to suppress the growth of F. oxysporum f.sp. vanillae effectively, with minimum inhibition concentration (MIC of 0.05 %. The extract inhibited colony growth diameter and total mycelial dry weight.

  9. Inhibitory effects of silver zeolite on in vitro growth of fish egg pathogen, Saprolegnia sp.

    Directory of Open Access Journals (Sweden)

    Seyed Ali Johari

    2014-05-01

    Full Text Available Objective: To investigate the effects of powdered silver zeolite (SZ on the in vitro growth of the fish pathogen Saprolegnia sp. Methods: The antifungal activity of SZ was evaluated by determining the minimum inhibitory concentrations using two-fold serial dilutions of powdered SZ in a glucose yeast extract agar at 22 °C. The growth of Saprolegnia sp. on the SZ agar treatments was compared to that on SZ-free agar controls. Results: The results showed that SZ had an inhibitory effect on the in vitro growth of the tested fungi. The minimum inhibitory concentration of SZ for Saprolegnia sp. was also calculated at 600 mg/L, which is equal to 0.06 percent. Conclusions: SZ is a potential good candidate to replace teratogenic and toxic agents, such as malachite green in aquaculture systems.

  10. Stolephorus sp Behavior in Different LED (Light Emitting Diode) Color and Light Intensities

    Science.gov (United States)

    Fitri Aristi, D. P.; Ramadanita, I. A.; Hapsari, T. D.; Susanto, A.

    2018-02-01

    This research aims to observe anchovy (Stolephorus sp) behavior under different LED light intensities that affect eye physiology (cell cone structure). The materials used were Stolephorus sp taken from the waters off Jepara and 13 and 10 watt light emitting diode (LED). The research method was an experiment conducted from March through August 2015 in the waters off Jepara. Data analysis of visual histology and fish respond was carried out at the fishing gear material laboratory, anatomy and cultivate. Cone cell structure (mosaic cone) of Stolephorus sp forms a connected regular square pattern with every single cone surrounded by four double cones, which indicate that anchovies are sensitive to light. The 13 watt LED (628 lux) has faster response than the 10 watt LED (531 lux) as it has wider and higher emitting intensity, which also attracts fish to gather quicker.

  11. A superhard sp3 microporous carbon with direct bandgap

    Science.gov (United States)

    Pan, Yilong; Xie, Chenlong; Xiong, Mei; Ma, Mengdong; Liu, Lingyu; Li, Zihe; Zhang, Shuangshuang; Gao, Guoying; Zhao, Zhisheng; Tian, Yongjun; Xu, Bo; He, Julong

    2017-12-01

    Carbon allotropes with distinct sp, sp2, and sp3 hybridization possess various different properties. Here, a novel all-sp3 hybridized tetragonal carbon, namely the P carbon, was predicted by the evolutionary particle swarm structural search. It demonstrated a low density among all-sp3 carbons, due to the corresponding distinctive microporous structure. P carbon is thermodynamically stable than the known C60 and could be formed through the single-walled carbon nanotubes (SWCNTs) compression. P carbon is a direct bandgap semiconductor displaying a strong and superhard nature. The unique combination of electrical and mechanical properties constitutes P carbon a potential superhard material for semiconductor industrial fields.

  12. New Eudesmane-Type Sesquiterpenoids from the Mangrove-Derived Endophytic Fungus Penicillium sp. J-54

    Directory of Open Access Journals (Sweden)

    Liuming Qiu

    2018-03-01

    Full Text Available Four new eudesmane-type sesquiterpenoids, penicieudesmol A–D (1–4, were isolated from the fermentation broth of the mangrove-derived endophytic fungus Penicillium sp. J-54. Their structures were determined by spectroscopic methods, the in situ dimolybdenum CD method, and modified Mosher’s method. The bioassays results showed that 2 exhibited weak cytotoxicity against K-562 cells.

  13. Structural Analysis of Planar sp3 and sp2 Films: Diamond-Like Carbon and Graphene Overlayers

    KAUST Repository

    Mansour, Ahmed

    2011-07-07

    The special electronic configuration of carbon enables the existence of wide ranging allotropes taking all possible dimensionalities. The allotropes of carbon are characterized by the type of hybridized bonding forming its structure, ranging from pure sp2 as in graphene, carbon nanotubes and fullerenes, to pure sp3 as in diamond. Amorphous and diamond-like carbon consists of a mixture of both hybridizations. This variation in hybridization in carbon materials enables a wide spectrum of properties, ranging from high bulk mechanical hardness, tribological properties and chemical inertness made possible by moving towards pure sp3 bonding to the extraordinary electrical conductivity, optical properties and in-plane mechanical strength resulting from pure sp2 bonding. Two allotropes at the extremes of this spectrum, diamond like carbon (DLC) and graphene, are investigated in this thesis; the former is investigated as a protective coating in hard drive applications, while the latter is investigated in the context of chemically derived graphene as material for transparent conducting electrode applications. DLC thin films are a main component in computer hard drives, acting as a protective coating against corrosion and mechanical wear of the magnetic layer and read-write head. The thickness of DLC films greatly affects the storage density in such devices, as larger separation between the read/write head and the magnetic layer decreases the storage density. A targeted DLC thickness of 2 nm would increase the storage density towards 1 Tbits/inch2. However, difficulty achieving continuous films at such thicknesses by commonly used sputtering methods challenges the industry to investigate alternative methods. Filtered cathodic vacuum arc (FCVA) has been proposed as an efficient technique to provide continuous, smooth and ultra-thin DLC films. We investigate the influence of deposition angle, deposition time, and substrate biasing to define the optimum process window to obtain

  14. Sp6 and Sp8 Transcription Factors Control AER Formation and Dorsal-Ventral Patterning in Limb Development

    Science.gov (United States)

    Haro, Endika; Delgado, Irene; Junco, Marisa; Yamada, Yoshihiko; Mansouri, Ahmed; Oberg, Kerby C.; Ros, Marian A.

    2014-01-01

    The formation and maintenance of the apical ectodermal ridge (AER) is critical for the outgrowth and patterning of the vertebrate limb. The induction of the AER is a complex process that relies on integrated interactions among the Fgf, Wnt, and Bmp signaling pathways that operate within the ectoderm and between the ectoderm and the mesoderm of the early limb bud. The transcription factors Sp6 and Sp8 are expressed in the limb ectoderm and AER during limb development. Sp6 mutant mice display a mild syndactyly phenotype while Sp8 mutants exhibit severe limb truncations. Both mutants show defects in AER maturation and in dorsal-ventral patterning. To gain further insights into the role Sp6 and Sp8 play in limb development, we have produced mice lacking both Sp6 and Sp8 activity in the limb ectoderm. Remarkably, the elimination or significant reduction in Sp6;Sp8 gene dosage leads to tetra-amelia; initial budding occurs, but neither Fgf8 nor En1 are activated. Mutants bearing a single functional allele of Sp8 (Sp6−/−;Sp8+/−) exhibit a split-hand/foot malformation phenotype with double dorsal digit tips probably due to an irregular and immature AER that is not maintained in the center of the bud and on the abnormal expansion of Wnt7a expression to the ventral ectoderm. Our data are compatible with Sp6 and Sp8 working together and in a dose-dependent manner as indispensable mediators of Wnt/βcatenin and Bmp signaling in the limb ectoderm. We suggest that the function of these factors links proximal-distal and dorsal-ventral patterning. PMID:25166858

  15. Analysis list: SP2 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SP2 Blood,Liver,Pluripotent stem cell + hg19 http://dbarchive.biosciencedbc.jp/kyus...hu-u/hg19/target/SP2.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/SP2.5.tsv http://dbarchive....biosciencedbc.jp/kyushu-u/hg19/target/SP2.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.Blood.tsv,http:...//dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.Liver.tsv,http:...//dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.Pluripotent_stem_cell.tsv http://dbarchive.biosciencedbc

  16. SP-100 space reactor power system readiness

    International Nuclear Information System (INIS)

    Josloff, A.T.; Matteo, D.N.; Bailey, H.S.

    1992-01-01

    This paper discusses the SP-100 Space Reactor Power System which is being developed by GE, under contract to the U.S. Department of Energy, to provide electrical power in the range of 10's to 100's of kW. The system represents an enabling technology for a wide variety of earth orbital and interplanetary science missions, nuclear electric propulsion (NEP) stages, and lunar/Mars surface power for the Space Exploration Initiative (SEI). The technology and design is now at a state of readiness to support the definition of early flight demonstration missions. Of particular importance is that SP-100 meets the demanding U.S. safety performance, reliability and life requirements. The system is scalable and flexible and can be configured to provide 10's to 100's of kWe without repeating development work and can meet DoD goals for an early, low-power demonstration flight in the 1996-1997 time frame

  17. Antimicrobial metabolites from the plant endophytic fungus Penicillium sp.

    Science.gov (United States)

    Yang, Ming-Hua; Li, Tian-Xiao; Wang, Ying; Liu, Rui-Huan; Luo, Jun; Kong, Ling-Yi

    2017-01-01

    Five rare dichloro aromatic polyketides (1-5) were obtained from an endophytic fungus Penicillium sp., along with five known metabolites (6-10). Their structures were elucidated by extensive spectroscopic analysis, Mosher methods, as well as [Rh 2 (OCOCF 3 ) 4 ]-induced electronic circular dichroism (ECD) experiments. Compounds 2-4 and 6 structurally involved acyclic 1.3-diols, the uneasy configuration determinations of which were well carried out by double-derivation NMR methods. Compounds 1-10 were evaluated for their antibacterial and antifungal activities against five strains of human pathogenic microorganisms. Helvolic acid (7) showed potent inhibitory effects against Staphylococcus aureus and Pseudomonas aeruginosa with MIC (minimum inhibitory concentration) values of 5.8 and 4.6μg/mL, respectively. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. Production of diketopiperazine derivative cyclo (l-leu-l-arg) by streptomyces sp. tn262 after exposure to heat-killed fungus fusarium sp

    International Nuclear Information System (INIS)

    Elleuch, L.; Smaoui, S.; Najah, S.; Sellem, I

    2013-01-01

    In a screening program for new active secondary metabolites producers, a strain of Streptomyces called TN262 was isolated from Tunisian soil and selected for its ability to produce eleven active compounds in pure culture conditions. In this work, the effect of different concentrations of heat-killed fungus Fusarium sp. on the production of active compounds by TN262 strain was studied. The ethyl acetate extract from the culture of Streptomyces sp. TN262 combined with heat-killed Fusarium sp. at 50 micro g/ml inhibited the growth of the three used indicator microorganisms. In fact, an increase of 36%, 21% and 20% in inhibitory activity was obtained against Micrococcus luteus LB 14110, Escherichia coli ATCC 8739 and Fusarium sp. respectively. The HPLC chromatographic profiles of the ethyl acetate extracts from both culture conditions were different and an additional active compound was produced only under induced conditions. This active component was isolated and identified as Cyclo (L-Leu-L-Arg) (1), a diketopiperazine derivative, possessing antibacterial and antifungal activity. Consequently, this study showed that the addition of heat-killed fungus is a useful method for inducing the production of bioactive compounds. (author)

  19. Superfield quantization in Sp(2) covariant formalism

    CERN Document Server

    Lavrov, P M

    2001-01-01

    The rules of the superfield Sp(2) covariant quantization of the arbitrary gauge theories for the case of the introduction of the gauging with the derivative equations for the gauge functional are generalized. The possibilities of realization of the expanded anti-brackets are considered and it is shown, that only one of the realizations is compatible with the transformations of the expanded BRST-symmetry in the form of super translations along the Grassmann superspace coordinates

  20. Spøgelsets egne ord

    DEFF Research Database (Denmark)

    Gemzøe, Anker

    2012-01-01

    refleksioner over den gengangerscene, der åbner Shakespeares Hamlet. Ifølge Derridas ’hantologie’ opererer spøgelser i en eksistentiel tidslighed, der har at gøre med sorg, sprog og forvandling. Genremæssigt, tilføjer Gemzøe, har gengangeren det med at husere i tragedien. Med Derridas ’hantologie’ som en af de...

  1. Carbon sp chains in graphene nanoholes

    International Nuclear Information System (INIS)

    Castelli, Ivano E; Ferri, Nicola; Onida, Giovanni; Manini, Nicola

    2012-01-01

    Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. We adopt a fair compromise of microscopic realism with a certain level of idealization in the model configurations, and predict a number of properties susceptible to comparison with experiment. (paper)

  2. Carbon sp chains in graphene nanoholes

    Science.gov (United States)

    Castelli, Ivano E.; Ferri, Nicola; Onida, Giovanni; Manini, Nicola

    2012-03-01

    Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. We adopt a fair compromise of microscopic realism with a certain level of idealization in the model configurations, and predict a number of properties susceptible to comparison with experiment.

  3. Commercial technologies from the SP-100 program

    International Nuclear Information System (INIS)

    Truscello, V.C.; Fujita, T.; Mondt, J.F.

    1995-01-01

    For more than a decade, Jet Propulsion Labortory and Los Alamos have managed a multi-agency funded effort to develop a space reactor power system. This SP-100 Program has developed technologies required for space power systems that can be implemented in the industrial and commercial sectors to improve competitiveness in the global economy. Initial steps taken to transfer this technology from the laboratories to industrial and commercial entities within United States include: (1) identifying specific technologies having commercial potential; (2) distributing information describing the identified technologies and interacting with interested commercial and industrial entities to develop application-specific details and requirements; and (3) providing a technological data base that leads to transfer of technology or the forming of teaming arrangements to accomplish the transfer by tailoring the technology to meet application-specific requirements. SP-100 technologies having commercial potential encompass fabrication processes, devices, and components. Examples: a process for bonding refractory metals to graphite, a device to sense the position of an actuator and a component to enable rotating machines to operate without supplying lubrication (self-lubricating ball bearing). Shortly after the NASA Regional Technology Transfer Centers widely disseminated information covering SP-100 technologies, over one hundred expressions of interest were received, which indicate that there is a large potential benefit in transferring SP-100 technology. Interactions with industrial and commercial entities have identified a substantial need for creating teaming arrangements involving the interested entity and personnel from laboratories and their contractors, who have the knowledge and ability to tailor the technology to meet application-specific requirements. copyright 1995 American Institute of Physics

  4. Thermomucor abortosporangium sp. nov. (Fungi: Mucorales

    Directory of Open Access Journals (Sweden)

    A. Swathi Sri

    2012-03-01

    Full Text Available Thermomucor abortosporangium A. Swathi Sri & A. Subrahm., sp. nov. is described as the second species of Thermomucor. It differs from the type species of T. indicae-seudaticae in the branching pattern of sporangiophores, size and shape of sporangiospores and production of abortosporangia. Zygospores are reddish brown, globose, psilate, isogamic and formed at low (37°C temperature whilst the higher temperatures are prohibitive. The fungus grows well at 50°C on PDA, SMA and MEA.

  5. Infecting Pacific Herring with Ichthyophonus sp. in the laboratory

    Science.gov (United States)

    Hershberger, Paul; Hart, Lucas; Mackenzie, Ashley; Yanney, M.L.; Conway, Carla M.; Elliott, Diane G.

    2015-01-01

    The protistan parasite Ichthyophonus sp. occurs in coastal populations of Pacific Herring Clupea pallasii throughout the northeast Pacific region, but the route(s) by which these planktivorous fish become infected is unknown. Several methods for establishing Ichthyophonus infections in laboratory challenges were examined. Infections were most effectively established after intraperitoneal (IP) injections with suspended parasite isolates from culture or after repeated feedings with infected fish tissues. Among groups that were offered the infected tissues, infection prevalence was greater after multiple feedings (65%) than after a single feeding (5%). Additionally, among groups that were exposed to parasite suspensions prepared from culture isolates, infection prevalence was greater after exposure by IP injection (74%) than after exposure via gastric intubation (12%); the flushing of parasite suspensions over the gills did not lead to infections in any of the experimental fish. Although the consumption of infected fish tissues is unlikely to be the primary route of Ichthyophonus sp. transmission in wild populations of Pacific Herring, this route may contribute to abnormally high infection prevalence in areas where juveniles have access to infected offal.

  6. Infecting Pacific Herring with Ichthyophonus sp. in the Laboratory.

    Science.gov (United States)

    Hershberger, P K; Hart, L M; MacKenzie, A H; Yanney, M L; Conway, C M; Elliott, D G

    2015-12-01

    The protistan parasite Ichthyophonus sp. occurs in coastal populations of Pacific Herring Clupea pallasii throughout the northeast Pacific region, but the route(s) by which these planktivorous fish become infected is unknown. Several methods for establishing Ichthyophonus infections in laboratory challenges were examined. Infections were most effectively established after intraperitoneal (IP) injections with suspended parasite isolates from culture or after repeated feedings with infected fish tissues. Among groups that were offered the infected tissues, infection prevalence was greater after multiple feedings (65%) than after a single feeding (5%). Additionally, among groups that were exposed to parasite suspensions prepared from culture isolates, infection prevalence was greater after exposure by IP injection (74%) than after exposure via gastric intubation (12%); the flushing of parasite suspensions over the gills did not lead to infections in any of the experimental fish. Although the consumption of infected fish tissues is unlikely to be the primary route of Ichthyophonus sp. transmission in wild populations of Pacific Herring, this route may contribute to abnormally high infection prevalence in areas where juveniles have access to infected offal.

  7. Optimization of cellulase production by Penicillium sp.

    Science.gov (United States)

    Prasanna, H N; Ramanjaneyulu, G; Rajasekhar Reddy, B

    2016-12-01

    The production of cellulolytic enzymes (β-exoglucanase, β-endoglucanase and β-glucosidase) by Penicillium sp. on three different media in liquid shake culture conditions was compared. The organism exhibited relatively highest activity of endoglucanase among three enzymes measured at 7-day interval during the course of its growth on Czapek-Dox medium supplemented with 0.5 % (w/v) cellulose. Cellulose at 0.5 %, lactose at 0.5 %, sawdust at 0.5 %, yeast extract at 0.2 % as a nitrogen source, pH 5.0 and 30 °C temperature were found to be optimal for growth and cellulase production by Penicillium sp. Yields of Fpase, CMCase and β-glucosidase, attained on optimized medium with Penicillium sp. were 8.7, 25 and 9.52 U/ml, respectively with increment of 9.2, 5.9 and 43.8-folds over titers of the respective enzyme on unoptimised medium. Cellulase of the fungal culture with the ratio of β-glucosidase to Fpase greater than one will hold potential for biotechnological applications.

  8. Biosorption of 241Am by Candida sp

    International Nuclear Information System (INIS)

    Luo Shunzhong; Zhang Taiming; Liu Ning; Yang Yuanyou; Jin Jiannan; Hua Xinfeng

    2003-01-01

    The biosorption of radionuclide 241 Am from solutions by Candida sp., and the influences of experimental conditions on the adsorption were studied. The results showed that the adsorption equilibrium was achieved within 4h and the optimum pH=2. No significant differences on 241 Am biosorption were observed at 10-45 degree C, or challenged with Au 3+ or Ag + , even 1500 times or 4500 times over 241 Am, respectively. The adsorption rate could reach 97.8% by dry Candida sp. of 0.82 g/L in 241 Am solutions (pH=2) of 5.6-111 MBq/L (44.04-873.0 μg/L) (C 0 ), with maximum adsorption capacity (W) of 63.5 MBq/g (501.8 μg/g), implying that the removal of 241 Am by Candida sp. from solutions was feasible. The relationship between activities (C 0 ) and adsorption capacities (W) of 241 Am indicated that the biosorption process could be described by Langmuir adsorption isotherm

  9. Biosorption of americium-241 by Candida sp

    International Nuclear Information System (INIS)

    Luo Shunzhong; Zhang Taiming; Liu Ning; Yang Yuanyou; Jin Jiannan; Liao Jiali

    2003-01-01

    As an important radioisotope in nuclear industry and other fields, americium-241 is one of the most serious contamination concerns duo to its high toxicity and long half-life. In this experiment, the biosorption of 241 Am from solution by Candida sp., and the effects of various experimental conditions on the adsorption were investigated. The preliminary results showed that the adsorption of 241 Am by Candida sp. was efficient. 241 Am could be removed by Candida sp. of 0.82 g/L (dry weight) from 241 Am solutions of 5.6-111 MBq/L (44.3-877.2 μg/L)(C 0 ), with maximum adsorption rate (R) of 98% and maximum adsorption capacities (W) of 63.5 MBq/g biomass (dry weight) (501.8 μg/g). The biosorption equilibrium was achieved within 4 hour and the optimum pH was pH = 2. No significant differences on 241 Am adsorption were observed at 10 C-45 C, or in solutions containing Au 3+ or Ag + , even 1500 times or 4500 times above the 241 Am concentration, respectively. The relationship between concentrations and adsorption capacities of 241 Am indicated the biosorption process should be described by a Langmuir adsorption isotherm. (orig.)

  10. Synthetic lung surfactants containing SP-B and SP-C peptides plus novel phospholipase-resistant lipids or glycerophospholipids

    Directory of Open Access Journals (Sweden)

    Robert H. Notter

    2016-10-01

    Full Text Available Background This study examines the biophysical and preclinical pulmonary activity of synthetic lung surfactants containing novel phospholipase-resistant phosphonolipids or synthetic glycerophospholipids combined with Super Mini-B (S-MB DATK and/or SP-Css ion-lock 1 peptides that replicate the functional biophysics of surfactant proteins (SP-B and SP-C. Phospholipase-resistant phosphonolipids used in synthetic surfactants are DEPN-8 and PG-1, molecular analogs of dipalmitoyl phosphatidylcholine (DPPC and palmitoyl-oleoyl phosphatidylglycerol (POPG, while glycerophospholipids used are active lipid components of native surfactant (DPPC:POPC:POPG 5:3:2 by weight. The objective of the work is to test whether these novel lipid/peptide synthetic surfactants have favorable preclinical activity (biophysical, pulmonary for therapeutic use in reversing surfactant deficiency or dysfunction in lung disease or injury. Methods Surface activity of synthetic lipid/peptide surfactants was assessed in vitro at 37 °C by measuring adsorption in a stirred subphase apparatus and dynamic surface tension lowering in pulsating and captive bubble surfactometers. Shear viscosity was measured as a function of shear rate on a Wells-Brookfield micro-viscometer. In vivo pulmonary activity was determined by measuring lung function (arterial oxygenation, dynamic lung compliance in ventilated rats and rabbits with surfactant deficiency/dysfunction induced by saline lavage to lower arterial PO2 to <100 mmHg, consistent with clinical acute respiratory distress syndrome (ARDS. Results Synthetic surfactants containing 5:3:2 DPPC:POPC:POPG or 9:1 DEPN-8:PG-1 combined with 3% (by wt of S-MB DATK, 3% SP-Css ion-lock 1, or 1.5% each of both peptides all adsorbed rapidly to low equilibrium surface tensions and also reduced surface tension to ≤1 mN/m under dynamic compression at 37 °C. However, dual-peptide surfactants containing 1.5% S-MB DATK + 1.5% SP-Css ion-lock 1 combined with

  11. Preliminary screening of Ni(II metal tolerance and dye-decolorizing by Nocardiopsis sp. SD8

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    Ramasamy Thangaraj

    2016-04-01

    Full Text Available Objective: To reveal the screening of metal tolerance and dye-decolorizing of Nocardiopsis sp. Methods: NiSO4 and Congo red dye were used for evaluating the metal tolerance and dyedecolorizing of the randomly selected actinobacterial isolates. Results: Nocardiopsis sp. SD8 showed a better efficiency in Ni(II tolerance, though a longer lag phase was observed for this microorganism grown for 7 days in integrated mismatch negativity. Interestingly, we also found that Nocardiopsis sp. SD8 had dye-decolorizing, hemolytic, lipase and protease activity. Conclusions: The present results revealed the bioremediation of metal resistant and diverse properties of Nocardiopsis sp. SD8 and further investigations are needed to extract and identify the potent molecule.

  12. Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov. prevail in diverse enzootic transmission cycles.

    Science.gov (United States)

    Margos, Gabriele; Lane, Robert S; Fedorova, Natalia; Koloczek, Johannes; Piesman, Joseph; Hojgaard, Andrias; Sing, Andreas; Fingerle, Volker

    2016-03-01

    Two species of the genus Borrelia , Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov., were first described by Postic and co-workers on the basis of genetic analyses of several loci. Multilocus sequence analysis of eight housekeeping loci confirmed that these two Borrelia genomospecies are distinct members of the Borrelia burgdorferi sensu lato complex. B. bissettiae sp. nov. was initially described in transmission cycles involving Neotoma fuscipes wood rats and Ixodes pacificus ticks in California, and Neotoma mexicana and Ixodes spinipalpis in Colorado. The preferred host of B. californiensis sp. nov. appears to be the California kangaroo rat, Dipodomys californicus ; Ixodes jellisoni , I. spinipalipis and I. pacificus ticks are naturally infected with it. Thus, the ecological associations of the two genomospecies and their genetic distance from all other known Borrelia genomospecies species justify their description as separate genomospecies: B. bissettiae sp. nov. (type strain DN127 T  = DSM 17990 T  =  CIP 109136 T ) and B. californiensis (type strain CA446 T  = DSM 17989 T  = ATCC BAA-2689 T ).

  13. A putative serine protease, SpSsp1, from Saprolegnia parasitica is recognised by sera of rainbow trout, Oncorhynchus mykiss

    Science.gov (United States)

    Minor, Kirsty L.; Anderson, Victoria L.; Davis, Katie S.; Van Den Berg, Albert H.; Christie, James S.; Löbach, Lars; Faruk, Ali Reza; Wawra, Stephan; Secombes, Chris J.; Van West, Pieter

    2014-01-01

    Saprolegniosis, the disease caused by Saprolegnia sp., results in considerable economic losses in aquaculture. Current control methods are inadequate, as they are either largely ineffective or present environmental and fish health concerns. Vaccination of fish presents an attractive alternative to these control methods. Therefore we set out to identify suitable antigens that could help generate a fish vaccine against Saprolegnia parasitica. Unexpectedly, antibodies against S. parasitica were found in serum from healthy rainbow trout, Oncorhynchus mykiss. The antibodies detected a single band in secreted proteins that were run on a one-dimensional SDS-polyacrylamide gel, which corresponded to two protein spots on a two-dimensional gel. The proteins were analysed by liquid chromatography tandem mass spectrometry. Mascot and bioinformatic analysis resulted in the identification of a single secreted protein, SpSsp1, of 481 amino acid residues, containing a subtilisin domain. Expression analysis demonstrated that SpSsp1 is highly expressed in all tested mycelial stages of S. parasitica. Investigation of other non-infected trout from several fish farms in the United Kingdom showed similar activity in their sera towards SpSsp1. Several fish that had no visible saprolegniosis showed an antibody response towards SpSsp1 suggesting that SpSsp1 might be a useful candidate for future vaccination trial experiments. PMID:25088077

  14. EFEKTIFITAS DAYA HAMBAT BAKTERI Streptomyces sp TERHADAP Erwinia sp PENYEBAB PENYAKIT BUSUK REBAH PADA TANAMAN LIDAH BUAYA (Aloe barbadensis Mill

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    SARMILA TASNIM

    2013-05-01

    Full Text Available Streptomyces sp was conducted from December 2010 - June 2011 at the Laboratoryof Microbiology, Biology Department, Math and Science Faculty, UdayanaUniversity Bukit Jimbaran-Bali. Implementation stages of the research consisted ofisolation and testing of the antibiotic activity Streptomyces sp to inhibit growthbacterial pathogens Erwinia sp as a cause of disease in plants fallen foul (Soft rot ofAloe barbadensis Mill.The results of this study have eight isolates of Streptomyces spwith macroscopic and microscopic characters are varied. Furthermore, all isolateswere obtained and then tested against antibiotic activity to inhibit growth the bacteriaErwinia sp. Test results obtained by Streptomyces sp that has the most effective ininhibiting the ability of the bacteria Erwinia sp isolates are Streptomyces sp2for (45%.

  15. spMC: an R-package for 3D lithological reconstructions based on spatial Markov chains

    Science.gov (United States)

    Sartore, Luca; Fabbri, Paolo; Gaetan, Carlo

    2016-09-01

    The paper presents the spatial Markov Chains (spMC) R-package and a case study of subsoil simulation/prediction located in a plain site of Northeastern Italy. spMC is a quite complete collection of advanced methods for data inspection, besides spMC implements Markov Chain models to estimate experimental transition probabilities of categorical lithological data. Furthermore, simulation methods based on most known prediction methods (as indicator Kriging and CoKriging) were implemented in spMC package. Moreover, other more advanced methods are available for simulations, e.g. path methods and Bayesian procedures, that exploit the maximum entropy. Since the spMC package was developed for intensive geostatistical computations, part of the code is implemented for parallel computations via the OpenMP constructs. A final analysis of this computational efficiency compares the simulation/prediction algorithms by using different numbers of CPU cores, and considering the example data set of the case study included in the package.

  16. Nanoscale transformation of sp2 to sp3 of graphite by slow highly charged ion irradiation

    International Nuclear Information System (INIS)

    Meguro, T.; Hida, A.; Koguchi, Y.; Miyamoto, S.; Yamamoto, Y.; Takai, H.; Maeda, K.; Aoyagi, Y.

    2003-01-01

    Nanoscale transformation of electronic states by highly charged ion (HCI) impact on graphite surfaces is described. The high potential energy of slow HCI, which induces multiple emission of electrons from the surface, provides a strong modification of the electronic states of the local area upon graphite surfaces. The HCI impact and the subsequent surface treatment either by electron injection from a scanning tunneling microscopy tip or by He-Cd laser irradiation induce a localized transition from sp 2 to sp 3 hybridization in graphite, resulting in the formation of nanoscale diamond-like structures (nanodiamond) at the impact region. From Raman spectroscopic measurements on sp 2 related peaks, it is found that the HCI irradiation creates vacancy complexes in contrast to ions having a lower charge state, which generate single vacancies. It is of interest that a single impact of HCI creates one nanodiamond structure, suggesting potential applications of HCI in nanoscale material processing

  17. Stated preference methods using R

    CERN Document Server

    Aizaki, Hideo; Sato, Kazuo

    2014-01-01

    Stated Preference Methods Using R explains how to use stated preference (SP) methods, which are a family of survey methods, to measure people's preferences based on decision making in hypothetical choice situations. Along with giving introductory explanations of the methods, the book collates information on existing R functions and packages as well as those prepared by the authors. It focuses on core SP methods, including contingent valuation (CV), discrete choice experiments (DCEs), and best-worst scaling (BWS). Several example data sets illustrate empirical applications of each method with R

  18. Burkholderia humisilvae sp. nov., Burkholderia solisilvae sp. nov. and Burkholderia rhizosphaerae sp. nov., isolated from forest soil and rhizosphere soil.

    Science.gov (United States)

    Lee, Jae-Chan; Whang, Kyung-Sook

    2015-09-01

    Strains Y-12(T) and Y-47(T) were isolated from mountain forest soil and strain WR43(T) was isolated from rhizosphere soil, at Daejeon, Korea. The three strains grew at 10-55 °C (optimal growth at 28-30 °C), at pH 3.0-8.0 (optimal growth at pH 6.0) and in the presence of 0-4.0% (w/v) NaCl, growing optimally in the absence of added NaCl. On the basis of 16S rRNA gene sequence analysis, the three strains were found to belong to the genus Burkholderia, showing the closest phylogenetic similarity to Burkholderia diazotrophica JPY461(T) (97.2-97.7%); the similarity between the three sequences ranged from 98.3 to 98.7%. Additionally, the three strains formed a distinct group in phylogenetic trees based on the housekeeping genes recA and gyrB. The predominant ubiquinone was Q-8, the major fatty acids were C16 : 0 and C17  : 0 cyclo and the DNA G+C content of the novel isolates was 61.6-64.4 mol%. DNA-DNA relatedness among the three strains and the type strains of the closest species of the genus Burkholderia was less than 50%. On the basis of 16S rRNA, recA and gyrB gene sequence similarities, chemotaxonomic and phenotypic data, the three strains represent three novel species within the genus Burkholderia, for which the names Burkholderia humisilvae sp. nov. (type strain Y-12(T)= KACC 17601(T) = NBRC 109933(T) = NCAIM B 02543(T)), Burkholderia solisilvae sp. nov. (type strain Y-47(T) = KACC 17602(T)= NBRC 109934(T) = NCAIM B 02539(T)) and Burkholderia rhizosphaerae sp. nov. (type strain WR43(T) = KACC 17603(T) = NBRC 109935(T) = NCAIM B 02541(T)) are proposed.

  19. A novel endophytic Huperzine A-producing fungus, Shiraia sp. Slf14, isolated from Huperzia serrata.

    Science.gov (United States)

    Zhu, D; Wang, J; Zeng, Q; Zhang, Z; Yan, R

    2010-10-01

    To characterize and identify a novel Huperzine A (HupA)-producing fungal strain Slf14 isolated from Huperzia serrata (Thunb. ex Murray) Trev. in China. The isolation, identification and characterization of a novel endophytic fungus producing HupA specifically and consistently from the leaves of H. serrata were investigated. The fungus was identified as Shiraia sp. Slf14 by molecular and morphological methods. The HupA produced by this endophytic fungus was shown to be identical to authentic HupA analysed by thin layer chromatographic, High-performance liquid chromatography (HPLC), LC-MS, (1) H NMR and acetylcholinesterase (AChE) inhibition activity in vitro. The amount of HupA produced by Shiraia sp. Slf14 was quantified to be 327.8 μg l(-1) by HPLC, which was far higher than that of the reported endophytic fungi, Acremonium sp., Blastomyces sp. and Botrytis sp. The production of HupA by endophyte Shiraia sp. Slf14 is an enigmatic observation. It would be interesting to further study the HupA production and regulation by the cultured endophyte in H. serrata and in axenic cultures. Although the current accumulation of HupA by the endophyte is not very high, it could provide a promising alterative approach for large-scale production of HupA. However, further strain improvement and the fermentation process optimization are required to result in the consistent and dependable production. © 2010 The Authors. Journal compilation © 2010 The Society for Applied Microbiology.

  20. Bifidobacterium reuteri sp. nov., Bifidobacterium callitrichos sp. nov., Bifidobacterium saguini sp. nov., Bifidobacterium stellenboschense sp. nov. and Bifidobacterium biavatii sp. nov. isolated from faeces of common marmoset (Callithrix jacchus) and red-handed tamarin (Saguinus midas).

    Science.gov (United States)

    Endo, Akihito; Futagawa-Endo, Yuka; Schumann, Peter; Pukall, Rüdiger; Dicks, Leon M T

    2012-03-01

    Five strains of bifidobacteria were isolated from faeces of a common marmoset (Callithrix jacchus) and a red-handed tamarin (Saguinus midas). The five isolates clustered inside the phylogenetic group of the genus Bifidobacterium but did not show high sequence similarities between the isolates and to known species in the genus by phylogenetic analysis based on 16S rRNA gene sequences. Sequence analyses of dnaJ1 and hsp60 also indicated their independent phylogenetic positions to each other in the Bifidobacterium cluster. DNA G+C contents of the species ranged from 57.3 to 66.3 mol%, which is within the values recorded for Bifidobacterium species. All isolates showed fructose-6-phosphate phosphoketolase activity. Based on the data provided, the five isolates represent five novel species, for which the names Bifidobacterium reuteri sp. nov. (type strain: AFB22-1(T) = JCM 17295(T) = DSM 23975(T)), Bifidobacterium callitrichos sp. nov. (type strain: AFB22-5(T) = JCM 17296(T) = DSM 23973(T)), Bifidobacterium saguini sp. nov. (type strain: AFB23-1(T) = JCM 17297(T) = DSM 23967(T)), Bifidobacterium stellenboschense sp. nov. (type strain: AFB23-3(T) = JCM 17298(T) = DSM 23968(T)) and Bifidobacterium biavatii sp. nov. (type strain: AFB23-4(T) = JCM 17299(T) = DSM 23969(T)) are proposed. Copyright © 2011 Elsevier GmbH. All rights reserved.

  1. Fermentation of Azolla sp. leaves and the utilization as a feed ingredient of tilapia Oreochromis sp.

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    Nur Bambang Priyo Utomo

    2015-03-01

    Full Text Available ABSTRACTThe objective of this study was to study the effect of incubation period on the nutritional content of Azolla sp. meal fermented by Trichoderma harzianum, and its optimum supplementation level in the feed of tilapia Oreochromis sp. In incubation period treatments, fermentation of Azolla meal was performed in two, six, eight, and ten days (AF2, AF6, AF8, AF10 using Trichoderma harzianum as the fermentor. The fish used in this study was tilapia Oreochromis sp. with an average weight of 10.59±1.29 g. The design of the feeding treatments was repeletting commercial feed with Azolla leaves by with different supplementation levels, i.e. 0% (A/control, 30% (B, 60% (C, and 90% (D. Faecal collection for digestibility measurement was conducted for 15 days and fish growth rate was observed for 40 days. Azolla meal fermented for two days (AF2 showed the best results among the other treatments with a crude fiber decrease of 37.19% and protein increase of 38.65%. The results of this study indicate that fermentation can increase the nutritional quality of Azolla meal and its most optimal supplementation level in the diet of tilapia is 30%.Keywords: crude fiber, Azolla sp., tilapiaABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh lama waktu fermentasi daun mata lele Azolla sp. menggunakan kapang Trichoderma harzianum serta dosis optimal dalam pakan ikan nila Oreochromis sp. Proses fermentasi tepung daun mata lele dilakukan selama dua, enam, delapan, dan sepuluh hari (AF2, AF6, AF8, AF10. Ikan uji pada penelitian ini menggunakan ikan nila Oreochromis sp. dengan bobot rata-rata 10,59±1,29 g yang ditebar sebanyak 6 ekor/akuarium berukuran 50×45×30 cm3. Sebagai pakan perlakuan yakni repeletting daun mata lele dengan pakan komersil pada tingkat suplementasi 0% (A/kontrol, 30% (B, 60% (C, dan 90% (D. Pemeliharaan ikan uji dan pengumpulan data dilakukan dengan mengumpulkan feses ikan untuk uji ketercernaan selama 15 hari dan mengamati pertumbuhan

  2. Isolation of C11 Cyclopentenones from Two Didemnid Species, Lissoclinum sp. and Diplosoma sp.

    Directory of Open Access Journals (Sweden)

    Katsuhiro Ueda

    2009-12-01

    Full Text Available A series of new C11 cyclopentenones 1-7 was isolated, together with four known metabolites 9/10, 12 and 13, from the extract of the didemnid ascidian Lissoclinum sp. The other didemnid ascidian Diplosoma sp. contained didemnenones 1, 2 and 5, and five known metabolites 8-12. The structures of 1-7 were elucidated by spectroscopic analyses. Cytotoxicity of the isolated compounds was evaluated against three human cancer cell lines (HCT116, A431 and A549.

  3. Screening of marine algae (Padina sp. from the Lengeh Port, Persian Gulf for antibacterial and antifungal activities

    Directory of Open Access Journals (Sweden)

    Azadeh Taherpour

    2016-09-01

    Full Text Available Objective: To evaluate the antibacterial efficacy of different solvent extracts of Padina sp. against selected human pathogenic bacteria and fungi species such as Escherichia coli, Shigella sp., Staphylococcus aureus (S. aureus, Pseudomonas aeruginosa, Aspergillus flavus and Candida albicans. Methods: Various solvents including methanol, ethyl acetate, chloroform and hexane were used to acquire crude extracts from marine algae Padina sp. After crude preparation, antibacterial and antifungal activities were screened against clinically important human pathogenic bacteria using disc and well diffusion methods. For all the bacterial species used in this research, minimum inhibitory concentration was undertaken considering various solvent extracts of Padina sp. To ensure the accuracy of experiments, a positive control was also included. Results: Confirmed that hexane is the best solvent to extract antimicrobial agents from Padina sp. Among selected bacteria, S. aureus was the most sensitive test microorganism. While, all other microorganisms showed resistance against methanol, ethyl acetate, chloroform extracts. In fact, by increasing concentration of hexane extract, inhibition of S. aureus growth or antimicrobial activity was increased. Growth inhibition zone in well method showed better results compared to disc diffusion method. The minimum inhibitory concentration and minimum bactericidal concentration of hexane extract were 15 and 30 mg/mL against S. aureus, respectively. All Padina sp. extracts did not reveal any antifungal activities against fungi species in this study. Conclusions: Brown algae extracts showed sufficient antibacterial properties against S. aureus. Therefore, Padina sp. in this research can be a good candidate to design and manufacture novel antibacterial agents used in pharmaceutical industries.

  4. Improvement in solvent tolerance by exogenous glycerol in Pseudomonas sp. BCNU 106.

    Science.gov (United States)

    Choi, H J; Lim, B R; Park, Y J; Joo, W H

    2017-08-01

    Solvent hypertolerant Pseudomonas sp. BCNU 106 still has some underlying growth limitation in solvents. Therefore, efficient mass cultivation methods are needed to pursue its applications in biotechnology. Pseudomonas sp. BCNU 106 was cultured in a medium supplemented with 0·05 mol l -1 glycerol and cell survival was monitored during its cultivation in the presence of 1% (v/v) toluene. Exogenously supplemented glycerol provided more protection against damage caused by toluene stress and conferred higher solvent tolerance of Pseudomonas sp. BCNU 106 to toluene compared to control Pseudomonas sp. BCNU 106 without the supplementation of glycerol. This low-cost mass cultivation method can be used to efficiently apply solvent-tolerant bacteria in biotransformation and biodegradation. Protection against toluene and improvement in bacterial cell growth by supplementation of glycerol in the presence of toluene are demonstrated in this study. This result can be used to solve growth-related hindrances of solvent-tolerant bacteria and establish their low-cost mass cultivation, thereby broadening their industrial and environmental applications. © 2017 The Society for Applied Microbiology.

  5. Simple Extraction and Molecular Weight Characterization of Fucoidan From Indonesian Sargassum SP.

    OpenAIRE

    Junaidi, Lukman

    2013-01-01

    Fucoidan is a complex polysaccharide compounds found in brown algae. Fucoidan exhibits various biological properties for disease prevention. There are various methods used to extract fucoidan from brown algae, such as using ethanol, acetone, HCl and microwave. This research aims to extract and characterize fucoidan from Sargassum sp. using simple methods with the variables on extracting solutions, temperature and time of extraction. Extraction solution used were water and HCl. Ttemperature us...

  6. Cobalt-Catalyzed, Aminoquinoline-Directed sp2 C-H Bond Alkenylation by Alkynes**

    Science.gov (United States)

    Grigorjeva, Liene; Daugulis, Olafs

    2014-01-01

    We have developed a method for cobalt-catalyzed, aminoquinoline- and picolinamide-directed sp2 C-H bond alkenylation by alkynes. Method shows excellent functional group tolerance and both internal and terminal alkynes are competent substrates for the coupling. The reaction employs Co(OAc)2*4H2O catalyst, Mn(OAc)2 cocatalyst, and oxygen from air as a terminal oxidant. PMID:25060365

  7. Role of surfactant protein A (SP-A)/lipid interactions for SP-A functions in the lung.

    Science.gov (United States)

    Casals, C

    2001-01-01

    Surfactant protein A (SP-A), an oligomeric glycoprotein, is a member of a group of proteins named collectins that contain collagen-like and Ca(2+)-dependent carbohydrate recognition domains. SP-A interacts with a broad range of amphipathic lipids (glycerophospholipids, sphingophospholipids, glycosphingolipids, lipid A, and lipoglycans) that are present in surfactant or microbial membranes. This review summarizes SP-A/lipid interaction studies regarding the lipid system used (i.e., phospholipid vesicles, phospholipid monolayers, and lipids immobilized on silica or adsorbed on a solid support). The effect of calcium, ionic strength, and pH on the binding of SP-A to lipids and the subsequent lipid aggregation process is discussed. Current evidence suggests that hydrophobic-binding forces are involved in the peripherical association of SP-A to membranes. It is also proposed that fluid and liquid-ordered phase coexistence in surfactant membranes might favor partition of SP-A into those membranes. The binding of SP-A to surfactant membranes containing hydrophobic surfactant peptides makes possible the formation of a membrane reservoir in the alveolar fluid that is protected by SP-A against inactivation and improves the rate of surfactant film formation. In addition, the interaction of SP-A with membranes might enhance the affinity of SP-A for terminal carbohydrates of glycolipids or glycoproteins on the surface of invading microorganisms.

  8. Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource.

    Directory of Open Access Journals (Sweden)

    Akanksha Srivastava

    Full Text Available An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732 were isolated (paddy fields and ponds in the Banaras Hindu University, campus and five strains screened for anticancer potential using human colon adenocarcinoma (HT29 and human kidney adenocarcinoma (A498 cancer cell lines. Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 were the most potent as determined by examination of morphological features and by inhibition of growth by graded concentrations of crude extracts and thin-layer chromatography (TLC eluates. Cell cycle analysis and multiplex assays using cancer biomarkers also confirmed Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as cancer drug resources. Apoptotic studies in the cells of A498 (cancer and MCF-10A (normal human epithelial exposed to crude extracts and TLC fractions revealed no significant impact on MCF-10A cells emphasizing its importance in the development of anticancer drug. Identification of biomolecules from these extracts are in progress.

  9. Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource.

    Science.gov (United States)

    Srivastava, Akanksha; Tiwari, Ratnakar; Srivastava, Vikas; Singh, Tej Bali; Asthana, Ravi Kumar

    2015-01-01

    An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732) were isolated (paddy fields and ponds in the Banaras Hindu University, campus) and five strains screened for anticancer potential using human colon adenocarcinoma (HT29) and human kidney adenocarcinoma (A498) cancer cell lines. Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 were the most potent as determined by examination of morphological features and by inhibition of growth by graded concentrations of crude extracts and thin-layer chromatography (TLC) eluates. Cell cycle analysis and multiplex assays using cancer biomarkers also confirmed Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as cancer drug resources. Apoptotic studies in the cells of A498 (cancer) and MCF-10A (normal human epithelial) exposed to crude extracts and TLC fractions revealed no significant impact on MCF-10A cells emphasizing its importance in the development of anticancer drug. Identification of biomolecules from these extracts are in progress.

  10. Role of religion and spirituality in medical patients: Confirmatory results with the SpREUK questionnaire

    Directory of Open Access Journals (Sweden)

    Ostermann Thomas

    2005-02-01

    Full Text Available Abstract Background Spirituality has become a subject of interest in health care as it is was recognized to have the potential to prevent, heal or cope with illness. There is less doubt that values and goals are important contributors to life satisfaction, physical and psychological health, and that goals are what gives meaning and purpose to people's lives. However, there is as yet but limited understanding of how patients themselves view the impact of spirituality on their health and well-being, and whether they are convinced that their illness may have "meaning" to them. To raise these questions and to more precisely survey the basic attitudes of patients with severe diseases towards spirituality/religiosity (SpR and their adjustment to their illness, we developed the SpREUK questionnaire. Methods In order to re-validate our previously described SpREUK instrument, reliability and factor analysis of the new inventory (Version 1.1 were performed according to the standard procedures. The test sample contained 257 German subjects (53.3 ± 13.4 years with cancer (51%, multiple sclerosis (24%, other chronic diseases (16% and patients with acute diseases (7%. Results As some items of the SpREUK construct require a positive attitude towards SpR, these items (item pool 2 were separated from the others (item pool 1. The reliability of the 15-item the construct derived from the item pool 1 respectively the 14-item construct which refers to the item pool 2 both had a good quality (Cronbach's alpha = 0.9065 resp. 0.9525. Factor analysis of item pool 1 resulted in a 3-factor solution (i.e. the 6-item sub-scale 1: "Search for meaningful support"; the 6-item sub-scale 2: "Positive interpretation of disease"; and the 3-item sub-scale 3: "Trust in external guidance" which explains 53.8% of variance. Factor analysis of item pool 2 pointed to a 2-factor solution (i.e. the 10-item sub-scale 4: "Support in relations with the External life through SpR" and the 4

  11. Neuroprotective Compound from an Endophytic Fungus, Colletotrichum sp. JS-0367.

    Science.gov (United States)

    Song, Ji Hoon; Lee, Changyeol; Lee, Dahae; Kim, Soonok; Bang, Sunghee; Shin, Myoung-Sook; Lee, Jun; Kang, Ki Sung; Shim, Sang Hee

    2018-05-23

    Colletotrichum sp. JS-0367 was isolated from Morus alba (mulberry), identified, and cultured on a large scale for chemical investigation. One new anthraquinone (1) and three known anthraquinones (2-4) were isolated and identified using spectroscopic methods including 1D/2D-NMR and HRESIMS. Although the neuroprotective effects of some anthraquinones have been reported, the biological activities of the four anthraquinones isolated in this study have not been reported. Therefore, the neuroprotective effects of these compounds were determined against murine hippocampal HT22 cell death induced by glutamate. Compound 4, evariquinone, showed strong protective effects against HT22 cell death induced by glutamate by the inhibition of intracellular ROS accumulation and Ca 2+ influx triggered by glutamate. Immunoblot analysis revealed that compound 4 reduced the phosphorylation of MAPKs (JNK, ERK1/2, and p38) induced by glutamate. Furthermore, compound 4 strongly attenuated glutamate-mediated apoptotic cell death.

  12. Pericocins A-D, New Bioactive Compounds from Periconia sp.

    Science.gov (United States)

    Wu, Yue-Hua; Xiao, Gao-Keng; Chen, Guo-Dong; Wang, Chuan-Xi; Hu, Dan; Lian, Yun-Yang; Lin, Feng; Guo, Liang-Dong; Yao, Xin-Sheng; Gao, Hao

    2015-12-01

    One new dihydroisocoumarin, pericocin A (1), one new chromone, pericocin B (2), and two new α-pyrone derivatives, pericocins C-D (3-4), together with two known compounds, 3-(2-oxo-2H-pyran-6-yl)propanoic acid (5) and (E)-3-(2-oxo-2H-pyran-6-yl)acrylic acid (6), were isolated from the culture of the endolichenic fungus Periconia sp.. Their structures were elucidated by spectroscopic methods. All these compounds are derived from the polyketone biosynthetic pathway. Compound 1 was obtained as a mixture of enantiomers. The antimicrobial activity of compounds 1-5 was tested against Escherichia coli, Staphylococcus aureus, Aspergillus niger, and Candida albicans. Compounds 1-5 showed moderate antimicrobial activity against A. niger and weak activity against C. albicans.

  13. Characterization of Wastes from Pasteurizadora Sancti Spíritus.

    Directory of Open Access Journals (Sweden)

    Yolanda Margarita Carbonell Cabarga

    2012-04-01

    Full Text Available The present work is about the characterization of wastes from Pasteurizadora Sancti Spíritus and their influence on the emission of wastes from the other companies that pour them to the same oxidation lagoons. Its objectives are the following: Initial inspection of the treatment system, study and assessment of the environmental impacts per production line, assessment of the emissions of liquid and solid wastes and their destination, identification of chemicals, fuels and lubricants, characterization of the liquid wastes during the last 20 years. In the Materials and Methods section it was carried out a study and assessment of the environmental impacts generated by the organization, as well as a description of its solid wastes. Besides, the liquid wastes were characterized during 20 years, reaching the conclusion that the wastes resulting from the productions incorporated to the treatment system such as Nela and the Meat Enterprise´s productions remain biodegradable.

  14. Biodiesel production from yeast Cryptococcus sp. using Jerusalem artichoke.

    Science.gov (United States)

    Sung, Mina; Seo, Yeong Hwan; Han, Shin; Han, Jong-In

    2014-03-01

    Jerusalem artichoke was investigated as a cheap substrate for the heterotrophic production using a lab yeast strain Cryptococcus sp. Using Response Surface Method, 54.0% of fructose yield was achieved at 12% of dried Jerusalem artichoke powder, 0.57% of nitric acid concentration, 117°C of reaction temperature, and 49min of reaction time. At this optimal condition, nitric acid showed the best catalytic activity toward inulin hydrolysis and also the resulting fructose hydrolyte supported the highest microbial growth compared with other acids. In addition, lipid productivity of 1.73g/L/d was achieved, which is higher than a defined medium using pure fructose as a substrate. Lipid quality was also found to be generally satisfactory as a feedstock for fuel, demonstrating Jerusalem artichoke could indeed be a good and cheap option for the purpose of biodiesel production. Copyright © 2013 Elsevier Ltd. All rights reserved.

  15. Three new amides from streptomyces sp. H7372

    Energy Technology Data Exchange (ETDEWEB)

    Cheenpracha, Sarot; Borris, Robert P.; Tran, Tammy T.; Chang, Leng Chee, E-mail: lengchee@hawaii.ed [University of Hawaii Hilo, HI (United States). College of Pharmacy. Dept. of Pharmaceutical Sciences; Jee, Jap Meng; Seow, Heng Fong; Cheah, Hwen-Yee [Universiti Putra Malaysia, Selangor (Malaysia). Faculty of Medicine and Health Sciences. Department of Pathology. bImmunology Unit; Hoc, Coy Choke [University Malaysia Sabah (Malaysia). School of Science and Technology. Biotechnology Program

    2011-07-01

    Three new amides, methyl phenatate A (1), actiphenamide (2) and actiphenol 1-beta-D-glucopyranoside (3), along with thirteen known compounds, were isolated from the organic extract of a fermentation culture of Streptomyces sp. H7372. The structures were elucidated by spectroscopic methods including 1D- and 2D-NMR techniques, and MS analyses. Cycloheximide (6) and cyclo({Delta}Ala-L-Val) (8) gave a clear zone of inhibition of Ras-Raf-1 interaction in the yeast two hybrid assay which showed high potency with 10 and 25 mm clear ZOIs on SD His{sup -} and inactive on SD His{sup +} at 2.5 mug per disk, respectively. (author)

  16. Lactobacillus nantensis sp. nov., isolated from French wheat sourdough.

    Science.gov (United States)

    Valcheva, Rosica; Ferchichi, Mounir F; Korakli, Maher; Ivanova, Iskra; Gänzle, Michael G; Vogel, Rudi F; Prévost, Hervé; Onno, Bernard; Dousset, Xavier

    2006-03-01

    A polyphasic taxonomic study of the bacterial flora isolated from traditional French wheat sourdough, using phenotypic characterization and phylogenetic as well as genetic methods, revealed a consistent group of isolates that could not be assigned to any recognized species. These results were confirmed by randomly amplified polymorphic DNA and amplified fragment length polymorphism fingerprinting analyses. Cells were Gram-positive, homofermentative rods. Comparative 16S rRNA gene sequence analysis of the representative strain LP33T indicated that these strains belong to the genus Lactobacillus and that they formed a branch distinct from their closest relatives Lactobacillus farciminis, Lactobacillus alimentarius, Lactobacillus paralimentarius and Lactobacillus mindensis. DNA-DNA reassociation experiments with the three phylogenetically closest Lactobacillus species confirmed that LP33T (= DSM 16982T = CIP 108546T = TMW 1.1265T) represents the type strain of a novel species, for which the name Lactobacillus nantensis sp. nov. is proposed.

  17. Determination of pyrrolizidine alkaloids in commercial comfrey products (Symphytum sp.).

    Science.gov (United States)

    Betz, J M; Eppley, R M; Taylor, W C; Andrzejewski, D

    1994-05-01

    The presence of hepatotoxic pyrrolizidine alkaloids in comfrey (Symphytum sp.) and the widespread use of decoctions of this plant as a beverage (herbal tea) are of increasing concern. A method for the extraction and solid-phase concentration and capillary gas chromatographic determination of these alkaloids and their N-oxides in botanical materials has been developed and was applied to eleven comfrey-containing products purchased from retail health-food outlets in the Washington, DC, area during May-June 1989. Nine of the 11 products were found to contain measurable quantities of one or more of the alkaloids, in ranges from 0.1 to 400.0 ppm. Products containing comfrey leaf in combination with one or more other ingredients were found to contain the lowest alkaloid levels. Highest levels were found in bulk comfrey root, followed by bulk comfrey leaf. The species of the bulk material was verified by thin-layer chromatography and other means.

  18. Dominancy of Trichodesmium sp. in the Biawak Island

    Science.gov (United States)

    Prihadi, D. J.

    2018-03-01

    The Biawak Island is one of the small islands in West Java Province with an abundance of marine biological resources. This research was conducted to collect the primary producer zooplankton and water quality parameters. Direct observation is done by field surveys and measurement in situ for plankton and environmental parameters such as temperature, water transparency, water current, salinity, dissolved oxygen, and pH. Trichodesmium sp. was found dominance in where some other types of zooplankton were found in the area, like Scenedesmus sp., Sagitta sp., Acartia sp. also occurred. Further, the most abundance of Trichodesmium sp. was found in southern of Biawak Island where mangroves, coral and seagrass ecosystem provide nutrients which indirectly support the abundance of planktons. Trichodesmium sp. is plankton that can survive in water with minimum nutrient.

  19. The SP Theory of Intelligence: An Overview

    Directory of Open Access Journals (Sweden)

    J Gerard Wolff

    2013-08-01

    Full Text Available This article is an overview of the SP theory of intelligence, which aims to simplify and integrate concepts across artificial intelligence, mainstream computing and human perception and cognition, with information compression as a unifying theme. It is conceived of as a brain-like system that receives "New" information and stores some or all of it in compressed form as "Old" information; and it is realised in the form of a computer model, a first version of the SP machine. The matching and unification of patterns and the concept of multiple alignment are central ideas. Using heuristic techniques, the system builds multiple alignments that are "good" in terms of information compression. For each multiple alignment, probabilities may be calculated for associated inferences. Unsupervised learning is done by deriving new structures from partial matches between patterns and via heuristic search for sets of structures that are "good" in terms of information compression. These are normally ones that people judge to be "natural", in accordance with the "DONSVIC" principle—the discovery of natural structures via information compression. The SP theory provides an interpretation for concepts and phenomena in several other areas, including "computing", aspects of mathematics and logic, the representation of knowledge, natural language processing, pattern recognition, several kinds of reasoning, information storage and retrieval, planning and problem solving, information compression, neuroscience and human perception and cognition. Examples include the parsing and production of language with discontinuous dependencies in syntax, pattern recognition at multiple levels of abstraction and its integration with part-whole relations, nonmonotonic reasoning and reasoning with default values, reasoning in Bayesian networks, including "explaining away", causal diagnosis, and the solving of a geometric analogy problem.

  20. Spanish version of the Oral Health Impact Profile (OHIP-Sp

    Directory of Open Access Journals (Sweden)

    Baelum Vibeke

    2006-07-01

    Full Text Available Abstract Background The need for appraisal of oral health-related quality of life has been increasingly recognized over the last decades. The aims of this study were to develop a Spanish version (OHIP-Sp of the Oral Health Impact Profile and to evaluate its convergent and discriminative validity, and its internal consistency. Methods The original 49-items OHIP was translated to Spanish, revised for understanding and semantics by two independent dentists, and then translated back to English by an independent bilingual dentist. The data originated in a cross sectional study conducted among high school students from the Province of Santiago, Chile. The study group was sampled using a multistage random cluster procedure yielding 9,203 students aged 12–21 years. All selected students were invited to participate and all filled a questionnaire with information on socio-demographic factors; oral health related behaviors; and self-reported oral health status (good, fair or poor. From this group, 9,163 students also accepted to fill a detailed questionnaire on socio-economic indicators and to receive a clinical examination comprising direct recordings of clinical attachment levels (CAL in molars and incisors, tooth loss, and the presence of necrotizing ulcerative gingival lesions. Results The participation rate and the questionnaire completeness were high with OHIP-Sp total scores being computed for 9,133 subjects. Self-perceived oral health status was associated with the total OHIP-Sp score and all its domains (Spearman rank correlation. The OHIP-Sp total score was also directly associated with the 4 dental outcomes investigated (Mann-Whitney test and the largest impact was found for the outcomes, 'tooth loss' with a mean OHIP-Sp score = 13.5 and 'CAL >= 3 mm' with a mean OHIP-Sp score = 13.0. Conclusion The OHIP-Sp revealed suitable convergent and discriminative validity and appropriate internal consistency (Cronbach's α. Further studies on OHIP-Sp

  1. Insulin utilizes the PI 3-kinase pathway to inhibit SP-A gene expression in lung epithelial cells

    Directory of Open Access Journals (Sweden)

    Snyder Jeanne M

    2002-10-01

    Full Text Available Abstract Background It has been proposed that high insulin levels may cause delayed lung development in the fetuses of diabetic mothers. A key event in lung development is the production of adequate amounts of pulmonary surfactant. Insulin inhibits the expression of surfactant protein A (SP-A, the major surfactant-associated protein, in lung epithelial cells. In the present study, we investigated the signal transduction pathways involved in insulin inhibition of SP-A gene expression. Methods H441 cells, a human lung adenocarcinoma cell line, or human fetal lung explants were incubated with or without insulin. Transcription run-on assays were used to determine SP-A gene transcription rates. Northern blot analysis was used to examine the effect of various signal transduction inhibitors on SP-A gene expression. Immunoblot analysis was used to evaluate the levels and phosphorylation states of signal transduction protein kinases. Results Insulin decreased SP-A gene transcription in human lung epithelial cells within 1 hour. Insulin did not affect p44/42 mitogen-activated protein kinase (MAPK phosphorylation and the insulin inhibition of SP-A mRNA levels was not affected by PD98059, an inhibitor of the p44/42 MAPK pathway. In contrast, insulin increased p70 S6 kinase Thr389 phosphorylation within 15 minutes. Wortmannin or LY294002, both inhibitors of phosphatidylinositol 3-kinase (PI 3-kinase, or rapamycin, an inhibitor of the activation of p70 S6 kinase, a downstream effector in the PI 3-kinase pathway, abolished or attenuated the insulin-induced inhibition of SP-A mRNA levels. Conclusion Insulin inhibition of SP-A gene expression in lung epithelial cells probably occurs via the rapamycin-sensitive PI 3-kinase signaling pathway.

  2. Cytotoxic and antibacterial naphthoquinones from an endophytic fungus, Cladosporium sp.

    Directory of Open Access Journals (Sweden)

    Md. Imdadul Huque Khan

    Full Text Available Objective: Endophytes have the potential to synthesize various bioactive secondary metabolites. The aim of the study was to find new cytotoxic and antibacterial metabolites from endophytic fungus, Cladosporium sp. isolated from the leaves of Rauwolfia serpentina (L. Benth. ex Kurz. (Fam: Apocyanaceae. Materials and methods: The endophytic fungus was grown on potato dextrose agar medium and extracted using ethyl acetate. Secondary metabolites were isolated by chromatographic separation and re-crystallization, and structures were confirmed by 1H NMR, 13C NMR and mass spectroscopic data. The cytotoxicity was determined by WST-1 assay and brine shrimp lethality bioassay, while antibacterial activity was assessed by disc diffusion method. Results: Two naphthoquinones, namely anhydrofusarubin (1 and methyl ether of fusarubin (2, were isolated from Cladosporium sp. The isolated compounds 1 and 2, by WST-1 assay against human leukemia cells (K-562 showed potential cytotoxicity with IC50 values of 3.97 μg/mL and 3.58 μg/mL, respectively. Initial screening of crude ethyl acetate extract and column fractions F-8 and F-10 exhibited noticeable cytotoxicity to brine shimp nauplii with LC50 values of 42.8, 1.2 and 2.1 μg/mL, respectively. Moreover, the isolated compound 2 (40 μg/disc showed prominent activities against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus megaterium with an average zone of inhibition of 27 mm, 25 mm, 24 mm and 22 mm, respectively and the activities were compared with kanamycin (30 μg/disc. Conclusion: Our findings indicate that anhydrofusarubin (1 and methyl ether of fusarubin (2 might be useful lead compounds to develop potential cytotoxic and antimicrobial drugs. Keywords: Endophytic fungi, Cladosporium species, Fusarubin, Cytoxicity, Antibacterial activity

  3. Methyl (Sp-2-(diphenylphosphinoferrocene-1-carboxylate

    Directory of Open Access Journals (Sweden)

    Petr Štěpnička

    2009-10-01

    Full Text Available The title compound, [Fe(C5H5(C19H16O2P], obtained serendipitously during recrystallization of 1-hydroxybenzotriazolyl (Sp-2-(diphenylphosphinoferrocene-1-carboxylate from methanol, crystallizes in the chiral space group P212121. Its crystal structure not only confirms the anticipated absolute configuration but also establishes a rather regular geometry for the ferrocene unit, devoid of any significant deformation due to the attached substituents. In the crystal, symmetry-related molecules are linked via weak C—H...O interactions.

  4. Increasing rice plant growth by Trichoderma sp.

    Science.gov (United States)

    Doni, Febri; Isahak, Anizan; Zain, Che Radziah Che Mohd; Sulaiman, Norela; Fathurahman, F.; Zain, Wan Nur Syazana Wan Mohd.; Kadhimi, Ahsan A.; Alhasnawi, Arshad Naji; Anhar, Azwir; Yusoff, Wan Mohtar Wan

    2016-11-01

    Trichoderma sp. is a plant growth promoting fungi in many crops. Initial observation on the ability to enhance rice germination and vigor have been reported. In this study, the effectiveness of a local isolate Trichoderma asprellum SL2 to enhance rice seedling growth was assessed experimentally under greenhouse condition using a completely randomized design. Results showed that inoculation of rice plants with Trichoderma asprellum SL2 significantly increase rice plants height, root length, wet weight, leaf number and biomass compared to untreated rice plants (control). The result of this study can serve as a reference for further work on the application of beneficial microorganisms to enhance rice production.

  5. SP-100 radiator design trade study

    International Nuclear Information System (INIS)

    Ewell, R.

    1992-01-01

    This paper reports on a design trade study of the SP-100 heat rejection subsystem (HRSS) which was made. A system code was used to evaluate the sensitivity of the HRSS mass and performance to changes. Variations in heat pipe diameter and cross-section, fin length and thickness, armor thickness, and overall configuration and materials were evaluated. The analysis indicates that the minimum system mass occurs for the case with many small diameter heat pipes, with ducting that maximizes the fraction of the heat pipe evaporator perimeter in contract with it

  6. Prepare for an SpR interview.

    Science.gov (United States)

    Garner, J P; Heppell, P S J

    2003-03-01

    By the time you attend an interview for a military SpR number you should have no real problems but it pays to be prepared. Begin preparations early, reading widely and talk to as many people as possible. Your consultants will have a useful viewpoint on the proceedings and may be able to help you refine your answers to the common questions. Arrive at your interview in a smart and timely fashion and answer questions with confidence and common sense. Avoid confrontation and bluff and be courteous at all times, whatever you may be feeling inside and thank the interview panel as you leave.

  7. Amino Alcohols from the Ascidian Pseudodistoma sp.

    Directory of Open Access Journals (Sweden)

    Tae Hyung Won

    2014-06-01

    Full Text Available Seven new amino alcohol compounds, pseudoaminols A–G (1–7, were isolated from the ascidian Pseudodistoma sp. collected off the coast of Chuja-do, Korea. Structures of these new compounds were determined by analysis of the spectroscopic data and from chemical conversion. The presence of an N-carboxymethyl group in two of the new compounds (6 and 7 is unprecedented among amino alcohols. Several of these compounds exhibited moderate antimicrobial activity and cytotoxicity, as well as weak inhibitory activity toward Na+/K+-ATPase.

  8. Indirect Manganese Removal by Stenotrophomonas sp. and Lysinibacillus sp. Isolated from Brazilian Mine Water

    Directory of Open Access Journals (Sweden)

    Natália Rocha Barboza

    2015-01-01

    Full Text Available Manganese is a contaminant in the wastewaters produced by Brazilian mining operations, and the removal of the metal is notoriously difficult because of the high stability of the Mn(II ion in aqueous solutions. To explore a biological approach for removing excessive amounts of aqueous Mn(II, we investigated the potential of Mn(II oxidation by both consortium and bacterial isolates from a Brazilian manganese mine. A bacterial consortium was able to remove 99.7% of the Mn(II. A phylogenetic analysis of isolates demonstrated that the predominant microorganisms were members of Stenotrophomonas, Bacillus, and Lysinibacillus genera. Mn(II removal rates between 58.5% and 70.9% were observed for Bacillus sp. and Stenotrophomonas sp. while the Lysinibacillus isolate 13P removes 82.7%. The catalytic oxidation of Mn(II mediated by multicopper oxidase was not properly detected; however, in all of the experiments, a significant increase in the pH of the culture medium was detected. No aggregates inside the cells grown for a week were found by electronic microscopy. Nevertheless, an energy-dispersive X-ray spectroscopy of the isolates revealed the presence of manganese in Stenotrophomonas sp. and Lysinibacillus sp. grown in K medium. These results suggest that members of Stenotrophomonas and Lysinibacillus genera were able to remove Mn(II by a nonenzymatic pathway.

  9. Biodegradation of hexavalent chromium (Cr+6) in wastewater using Pseudomonas sp. and Bacillus sp. bacterial strains

    Energy Technology Data Exchange (ETDEWEB)

    Qasim, Muhammad [Department of Chemical Engineering, American University of Sharjah (United Arab Emirates)

    2013-07-01

    The recovery of toxic metal compounds is a deep concern in all industries. Hexavalent chromium is particularly worrying because of its toxic influence on human health. In this paper, biodegradation of hexavalent chromium (Cr+6) present in wastewater has been studied using two different bacterial strains; Pseudomonas sp. and Bacillus sp. A chemostat (with and without recycle of cells) with 10 L liquid culture volume was used to study the substrate and the biomass cell concentrations with time. Also, the degree of substrate conversion was studied by the varying the dilution rate as an independent parameter. The dilution rate (ratio of feed flow rate to the culture volume) was varied by varying the feed volumetric rate from 110-170 mL/h for inlet hexavalent chromium concentrations of 70 mg/dm3. The results show that a chemostat with recycle gives a better performance in terms of substrate conversion than a chemostat without a recycle. Moreover, the degree of substrate conversion decreases as the dilution rate is increased. Also, Bacillus sp. was found to give higher conversions compared to pseudomonas sp.

  10. Genetic diversity in Fusarium oxysporum f.sp. dianthi and F. redolens f.sp. dianthi

    NARCIS (Netherlands)

    Baayen, R.P.; Dreven, van F.; Krijger, M.C.; Waalwijk, C.

    1997-01-01

    Pathogenic isolates were selected representing all known vegetative compatibility groups (VCGs) and races of Fusarium oxysporum sensu lato from Dianthus spp. On basis of differences in the internal transcribed spacer region of the ribosomal DNA, six VCGs were classified as F. oxysporum f.sp. dianthi

  11. SHEAR STRENGTH IN THE GLUE LINE OF Eucalyptus sp. AND Pinus sp.WOOD

    Directory of Open Access Journals (Sweden)

    Juliana Jerásio Bianche

    Full Text Available ABSTRACT To evaluate the adhesive efficiency on the union of glued joints in a particular temperature and humidity conditions for a specified time the adhesive must be submitted to specific load tests, such as shear in the glue line. The objective of this study was to evaluate the shear strength in the glue line of Eucalyptus sp and Pinus sp.woods. Five adhesives (castor oil, sodium silicate, modified silicate, , PVA and resorcinol-formaldehyde, three weights (150 g/m2, 200 g/m2, and 250 g/m2 and two species (Eucalyptus sp. and Pinus sp. of wood were used. Twelve specimens were obtained from each repetition per treatment, corresponding to 108 specimens that were conditioned at a temperature of 23 ± 1°C and relative humidity of 50 ± 2%. The interaction between the weight and type of adhesive was significant for the shear strength in the glue line of eucalyptus wood. However, no interaction between the weight and the adhesive was found for pinus, only the isolated from the adhesive effect. Chemical bonds originated in the polymerization of resorcinol-formaldehyde adhesives and castor bi-component conferred upon these adhesives the greatest resistance in the glue line. Castor and resorcinol-formaldehyde adhesives showed the highest shear strength values in the line of glue and wood failure. Castor adhesive presented satisfactory performance for bonding of eucalyptus and pine woods.

  12. Mineralization of a Malaysian crude oil by Pseudomonas sp. and Achromabacter sp. isolated from coastal waters

    Energy Technology Data Exchange (ETDEWEB)

    Ahmad, J.; Ahmad, M.F.

    1995-12-31

    Regarded as being a potentially effective tool to combat oil pollution, bioremediation involves mineralization, i.e., the conversion of complex hydrocarbons into harmless CO{sub 2} and water by action of microorganisms. Therefore, in achieving optimum effectiveness from the application of these products on crude oil in local environments, the capability of the bacteria to mineralize hydrocarbons was evaluated. The microbial laboratory testing of mineralization on local oil degraders involved, first, isolation of bacteria found at a port located on the west coast of Peninsular Malaysia. Subsequently, these bacteria were identified by means of Biomereux`s API 20E and 20 NE systems and later screened by their growth on a Malaysian crude oil. Selected strains of Pseudomonas sp. and Achromabacter sp. were then exposed individually to a similar crude oil in a mineralization unit and monitored for 16 days for release of CO{sub 2}. Pseudomonas paucimobilis was found to produce more CO{sub 2} than Achromobacter sp. When tested under similar conditions, mixed populations of these two taxa produced more CO{sub 2} than that produced by any individual strain. Effective bioremediation of local crude in Malaysian waters can therefore be achieved from biochemically developed Pseudomonas sp. strains.

  13. SP@CE - An SP-based programming model for consumer electronics streaming applications

    NARCIS (Netherlands)

    Varbanescu, Ana Lucia; Nijhuis, Maik; Escribano, Arturo González; Sips, Henk; Bos, Herbert; Bal, Henri

    2007-01-01

    Efficient programming of multimedia streaming applications for Consumer Electronics (CE) devices is not trivial. As a solution for this problem, we present SP@CE, a novel programming model designed to balance the specific requirements of CE streaming applications with the simplicity and efficiency

  14. Eficiencia de pseudomonas sp, rhodopseudomonas sp, micrococcus sp y bacillus sp empleados como cultivos individuales y en consorcio, en la degradación de petróleo diesel ii

    OpenAIRE

    Otiniano García, Nélida Milly Esther

    2010-01-01

    In order to evaluate the efficiency of Pseudomonas sp, Rhodopseudomonas sp, Micrococcus sp, Bacillus sp, and the consortium formed by these four microorganisms in the diesel II petroleum degradation, it was worked in 5 bioreactors of aerated and shaken tank of 1.5 litters of capacity, with speed agitation of 120 rpm, and air flow of 0.5 vvm; in which were placed; 940 mL of Minimum Broth of Davies pH 7.0; 50 mL of diesel II petroleum as source of carbon and 10 mL of a suspension of approx...

  15. Sp(2) covariant quantisation of general gauge theories

    Energy Technology Data Exchange (ETDEWEB)

    Vazquez-Bello, J L

    1994-11-01

    The Sp(2) covariant quantization of gauge theories is studied. The geometrical interpretation of gauge theories in terms of quasi principal fibre bundles Q(M{sub s}, G{sub s}) is reviewed. It is then described the Sp(2) algebra of ordinary Yang-Mills theory. A consistent formulation of covariant Lagrangian quantisation for general gauge theories based on Sp(2) BRST symmetry is established. The original N = 1, ten dimensional superparticle is considered as an example of infinitely reducible gauge algebras, and given explicitly its Sp(2) BRST invariant action. (author). 18 refs.

  16. Sp(2) covariant quantisation of general gauge theories

    International Nuclear Information System (INIS)

    Vazquez-Bello, J.L.

    1994-11-01

    The Sp(2) covariant quantization of gauge theories is studied. The geometrical interpretation of gauge theories in terms of quasi principal fibre bundles Q(M s , G s ) is reviewed. It is then described the Sp(2) algebra of ordinary Yang-Mills theory. A consistent formulation of covariant Lagrangian quantisation for general gauge theories based on Sp(2) BRST symmetry is established. The original N = 1, ten dimensional superparticle is considered as an example of infinitely reducible gauge algebras, and given explicitly its Sp(2) BRST invariant action. (author). 18 refs

  17. Enkapsulasi B. bassiana menggunakan maizena dan daya infeksinya terhadap larva Aedes aegypti, Anopheles sp., Culex sp.

    Directory of Open Access Journals (Sweden)

    Mutiara Widawati

    2015-12-01

    Full Text Available Abstract. Encapsulation formulae of mycoinsecticide have to be able to maintain fungus viability and pathogenicity. This mycoinsecticide was developed as an alternative way to control mosquito borne disease. The aim of this study was to encapsulate Beauveria bassiana as viable storage and have the capability to kill larvae of Aedes aegypti, Anopheles sp. and Culex sp. Mosquito larvae obtained from laboratory reared at health research laboratory; Loka Litbang P2B2 Ciamis. The treatments made in this study were the formu-lation of cornstarch and controls for comparison. This study showed potential formulation of cornstarch encapsulation as a biolarvacidal. Cornstarch formulations proven to be succeed in maintaining fungus viability, however, the pathogenicity of the microcapsule still not effective to kill Aedes, Culex and Anopheles larvae. Keywords: encapsulation, Beauveria bassiana, Ae.aegypti, Anopheles sp., Culex sp. Abstrak. Pembuatan formula bioinsektisida yang optimal sebagai salah satu alternatif untuk pengen-dalian nyamuk vektor perlu di kembangkan. Sediaan mikoinsektisida yang dibuat harus dapat memper-tahankan viabilitas jamur B. bassiana sehingga masih efektif pada saat penggunaannya. Salah satu cara yang digunakan untuk menjaga kestabilan sediaan mikoinsektisida yang berdampak langsung pada via-bilitas jamur adalah dengan menerapkan metode enkapsulasi. Penelitian ini bertujuan untuk meng-hasilkan sediaan mikokapsul dari Beauvaria bassiana melalui proses enkapsulasi menggunakan maizena yang memiliki kapabilitas tinggi sebagai penyimpan B. bassiana dan efektif dalam membunuh larva dan telur Aedes aegypti, Anopheles sp. dan Culex sp. Semua larva uji berasal dari insektarium laboratorium penelitian kesehatan Loka litbang P2B2 Ciamis. Pembuatan enkapsulasi dimulai dengan kultur dan pema-nenan B. bassiana, uji viabilitas, proses enkapsulasi serta uji larvasida di laboratorium. Uji dilakukan dengan satu perlakuan dan satu kontrol untuk

  18. Program and Abstracts of the International Symposium on Detection of Subsurface Flow Phenomena by Self-Potential/Geoelectrical and Thermical Methods Held in Karlsruhe, Federal Republic of Germany on March 14-18, 1988

    Science.gov (United States)

    1988-03-18

    The tests were carried out at Le Mayet-de-Montagne (Allier). This test site is used by I.N.A.G. (Institut National d’Astronomie et de Geophysique ...study. Dep. Geophysique , Bureau de Recherches Geologiques et Minieres, B.P. 6009, 45060 Orleans Cedex 2, France 41 - THE USE OF INFRARED THERMOGRAPHY

  19. UTILIZAÇÃO DE ADESIVO PVA EM COMPENSADOS DE Pinus sp. E Eucalyptus sp. / UTILIZATION OF PVA ADHESIVES IN Pinus sp. and Eucalyptus sp. PLYWOOD

    Directory of Open Access Journals (Sweden)

    C. I. De Campos

    2014-12-01

    Full Text Available Estudos sobre novos adesivos ou resinas para colagem de madeira e derivados estão sendo realizados com a intenção de diminuir o impacto ambiental sem alterar suas propriedades. Por este motivo, novas formulações de adesivos PVA (Acetato de Polivinila vêm sendo desenvolvidas. Este trabalho testou a utilização deste adesivo na produção de compensado de Eucalyptus sp. e Pinus sp., com tempo e temperatura de prensagem, respectivamente, de 10 minutos e90°C. Foram realizados os ensaios físicos de densidade, teor de umidade e absorção, e os ensaios mecânicos de flexão estática e de resistência ao cisalhamento na linha de cola, todos de acordo com as normas ABNT. Obtiveram-se bons resultados com relação à resistência ao cisalhamento na linha de cola, a qual foi superior para os adesivos PVA, se comparados com compensados produzidos com ureia-formaldeído e o fenol-formaldeído, enquanto que os resultados de MOE e MOR apresentaram-se inferiores em ambos os casos. 

  20. Burkholderia cordobensis sp. nov., from agricultural soils.

    Science.gov (United States)

    Draghi, Walter O; Peeters, Charlotte; Cnockaert, Margo; Snauwaert, Cindy; Wall, Luis G; Zorreguieta, Angeles; Vandamme, Peter

    2014-06-01

    Two Gram-negative, rod-shaped bacteria were isolated from agricultural soils in Córdoba province in central Argentina. Their 16S rRNA gene sequences demonstrated that they belong to the genus Burkholderia, with Burkholderia zhejiangensis as most closely related formally named species; this relationship was confirmed through comparative gyrB sequence analysis. Whole-cell fatty acid analysis supported their assignment to the genus Burkholderia. Burkholderia sp. strain YI23, for which a whole-genome sequence is available, represents the same taxon, as demonstrated by its highly similar 16S rRNA (100% similarity) and gyrB (99.1-99.7%) gene sequences. The results of DNA-DNA hybridization experiments and physiological and biochemical characterization further substantiated the genotypic and phenotypic distinctiveness of the Argentinian soil isolates, for which the name Burkholderia cordobensis sp. nov. is proposed, with strain MMP81(T) ( = LMG 27620(T) = CCUG 64368(T)) as the type strain. © 2014 IUMS.

  1. The Effect of Acupuncture to SP6 on Skin Temperature Changes of SP6 and SP10: An Observation of “Deqi”

    Directory of Open Access Journals (Sweden)

    Jia-Min Yang

    2014-01-01

    Full Text Available Background. Deqi sensation is a complex but an important component for acupuncture effect. In this study, we tried to observe the relationship between Deqi and skin temperature changes and whether there was some relativity between Deqi and needle stimulations on cold congealing and dysmenorrhea rat model. Thirty-two female Sprague Dawley (SD rats were randomly divided into four groups (Saline Control Group, Model Group, Group A with strong stimulation, and Group B with small stimulation. Group A and Group B were performed with different stimulations. We found that, compared with saline control group, model group, and Group B, Group A showed that the skin temperature changes on right acupoint SP6 and SP10 increased significantly at 5 min–10 min interval. The skin temperature changes on left SP6 decreased at instant–5 min interval. The skin temperature changes on right SP10 decreased significantly at instant–5 min interval and 10 min–20 min interval. Thermogenic action along Spleen Meridian of Foot Greater Yin was manifested as simultaneous skin temperature increase on right SP6 and SP10 at 5 min–10 min interval after needling SP6, which was helpful to illustrate the relationship between the characteristic of Deqi and needle stimulations.

  2. Scope and Limitations of Auxiliary-Assisted, Palladium-Catalyzed Arylation and Alkylation of sp2 and sp3 C-H Bonds

    Science.gov (United States)

    Nadres, Enrico T.; Santos, Gerson Ivan Franco; Shabashov, Dmitry; Daugulis, Olafs

    2013-01-01

    The scope of palladium-catalyzed, auxiliary-assisted direct arylation and alkylation of sp2 and sp3 C-H bonds of amine and carboxylic acid derivatives has been investigated. The method employs a palladium acetate catalyst, substrate, aryl, alkyl, benzyl, or allyl halide, and inorganic base in t-amyl alcohol or water solvent at 100-140 °C. Aryl and alkyl iodides as well as benzyl and allyl bromides are competent reagents in this transformation. Picolinic acid auxiliary is used for amine γ-functionalization and 8-aminoquinoline auxiliary is used for carboxylic acid β-functionalization. Some optimization of base, additives, and solvent is required for achieving best results. PMID:24090404

  3. Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains

    Directory of Open Access Journals (Sweden)

    Samiha Sioud

    2009-01-01

    Full Text Available Streptomyces sp. US 24 and Streptomyces sp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E. coli ET12567 (pUZ8002, as a conjugal donor, carrying the integrative plasmid pSET152. For the Streptomyces sp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp. TN 58, the integration was in single copy at the attB site. Plasmid pSET152 was inherited every time for all analysed Streptomyces sp. US 24 and Streptomyces sp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA from Streptomyces sp. SK was expressed in strain Streptomyces sp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of the Streptomyces sp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch.

  4. Biodegradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1.

    Science.gov (United States)

    Tallur, Preeti N; Mulla, Sikandar I; Megadi, Veena B; Talwar, Manjunatha P; Ninnekar, Harichandra Z

    2015-01-01

    Pyrethroid pesticide cypermethrin is a environmental pollutant because of its widespread use, toxicity and persistence. Biodegradation of such chemicals by microorganisms may provide an cost-effective method for their detoxification. We have investigated the degradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1 in various matrices such as, polyurethane foam (PUF), polyacrylamide, sodium alginate and agar. The optimum temperature and pH for the degradation of cypermethrin by immobilized cells of Micrococcus sp. were found to be 30 °C and 7.0, respectively. The rate of degradation of 10 and 20 mM of cypermethrin by freely suspended cells were compared with that of immobilized cells in batches and semi-continuous with shaken cultures. PUF-immobilized cells showed higher degradation of cypermethrin (10 mM and 20 mM) than freely suspended cells and cells immobilized in other matrices. The PUF-immobilized cells of Micrococcus sp. strain CPN 1 were retain their degradation capacity. Thus, they can be reused for more than 32 cycles, without losing their degradation capacity. Hence, the PUF-immobilized cells of Micrococcus sp. could potentially be used in the bioremediation of cypermethrin contaminated water.

  5. Bacterial cellulose synthesis mechanism of facultative anaerobe Enterobacter sp. FY-07.

    Science.gov (United States)

    Ji, Kaihua; Wang, Wei; Zeng, Bing; Chen, Sibin; Zhao, Qianqian; Chen, Yueqing; Li, Guoqiang; Ma, Ting

    2016-02-25

    Enterobacter sp. FY-07 can produce bacterial cellulose (BC) under aerobic and anaerobic conditions. Three potential BC synthesis gene clusters (bcsI, bcsII and bcsIII) of Enterobacter sp. FY-07 have been predicted using genome sequencing and comparative genome analysis, in which bcsIII was confirmed as the main contributor to BC synthesis by gene knockout and functional reconstitution methods. Protein homology, gene arrangement and gene constitution analysis indicated that bcsIII had high identity to the bcsI operon of Enterobacter sp. 638; however, its arrangement and composition were same as those of BC synthesizing operon of G. xylinum ATCC53582 except for the flanking sequences. According to the BC biosynthesizing process, oxygen is not directly involved in the reactions of BC synthesis, however, energy is required to activate intermediate metabolites and synthesize the activator, c-di-GMP. Comparative transcriptome and metabolite quantitative analysis demonstrated that under anaerobic conditions genes involved in the TCA cycle were downregulated, however, genes in the nitrate reduction and gluconeogenesis pathways were upregulated, especially, genes in three pyruvate metabolism pathways. These results suggested that Enterobacter sp. FY-07 could produce energy efficiently under anaerobic conditions to meet the requirement of BC biosynthesis.

  6. Isolation and characterization of mesotrione-degrading Bacillus sp. from soil

    International Nuclear Information System (INIS)

    Batisson, Isabelle; Crouzet, Olivier; Besse-Hoggan, Pascale; Sancelme, Martine; Mangot, Jean-Francois; Mallet, Clarisse; Bohatier, Jacques

    2009-01-01

    Dissipation kinetics of mesotrione, a new triketone herbicide, sprayed on soil from Limagne (Puy-de-Dome, France) showed that the soil microflora were able to biotransform it. Bacteria from this soil were cultured in mineral salt solution supplemented with mesotrione as sole source of carbon for the isolation of mesotrione-degrading bacteria. The bacterial community structure of the enrichment cultures was analyzed by temporal temperature gradient gel electrophoresis (TTGE). The TTGE fingerprints revealed that mesotrione had an impact on bacterial community structure only at its highest concentrations and showed mesotrione-sensitive and mesotrione-adapted strains. Two adapted strains, identified as Bacillus sp. and Arthrobacter sp., were isolated by colony hybridization methods. Biodegradation assays showed that only the Bacillus sp. strain was able to completely and rapidly biotransform mesotrione. Among several metabolites formed, 2-amino-4-methylsulfonylbenzoic acid (AMBA) accumulated in the medium. Although sulcotrione has a chemical structure closely resembling that of mesotrione, the isolates were unable to degrade it. - A Bacillus sp. strain isolated from soil was able to completely and rapidly biotransform the triketone herbicide mesotrione

  7. Penapisan Cendawan Antagonis Indigenos Rizosfer Jahe dan Uji Daya Hambatnya terhadap Fusarium oxysporum f. sp. zingiberi

    Directory of Open Access Journals (Sweden)

    Nurbailis Nurbailis

    2015-02-01

    Full Text Available Ginger rot disease caused by Fusarium oxysporum f. sp. zingiberi is difficult to control because the pathogen is soil borne and is able to form clamidospore as resting structure. The aim of this study was to obtain indigenous antagonistic fungi from ginger rhizosphere which is potential for suppressing the growth of F. oxysporum f. sp. zingiberi. Fungi isolated from ginger rhizosphere were subjected for antagonism assay using dual culture method. Fungi isolates showed capability to inhibit F. oxysporum f. sp. zingiberii were then identified based on morphology characters. Eleven isolates were successfully isolated, but only 9 isolates showed the potentials of suppressing the growth of F. oxysporum f. sp.  zingiberi. All 9 isolates i.e. AB4, GC1, BB1, AB1, AB2, K12, GC3, K11 and GC2 had antibiosis activity, and 3 isolates among them i.e. AB2, BB1 and K11 showed competition mechanism. Based on morphology characters the isolates were identified as Penicillium spp. (4 isolates, Trichoderma spp. (3 isolates, and Aspergillus spp. (2 isolates.

  8. The effect of Pseudoxanthomonas sp. as manganese oxidizing bacterium on the corrosion behavior of carbon steel

    International Nuclear Information System (INIS)

    Ashassi-Sorkhabi, H.; Moradi-Haghighi, M.; Zarrini, G.

    2012-01-01

    The present study investigated the role of manganese oxidizing bacterium (MOB), namely Pseudoxanthomonas sp. on the corrosion behavior of carbon steel. This bacterium was isolated from sewage treatment plants and identified by biochemical and molecular methods. The electrochemical techniques such as open circuit potentiometry, electrochemical impedance spectroscopy, potentiodynamic and cyclic polarization were used to measure the corrosion rate and observe the corrosion mechanism. Also, scanning electron microscopy and X-ray diffraction studies were applied to surface analysis. This study revealed the strong adhesion of the biofilm on the metal surface in the presence of Pseudoxanthomonas sp. that enhanced the corrosion of carbon steel. X-ray diffraction patterns identified a high content of MnO 2 deposition within these biofilms. This is the first report that discloses the involvement of Pseudoxanthomonas sp. as manganese oxidizing bacteria on the corrosion of carbon steel. - Highlights: ► A new type of manganese oxidizing bacteria, namely Pseudoxanthomonas sp. was indicated. ► This bacterium can create a biofilm on the part of metal surface and affect localized corrosion. ► In the presence of biofilm, the diffusion of oxygen vacancies and manganese ions has occurred.

  9. Antagonistic activity of antibiotic producing Streptomyces sp. against fish and human pathogenic bacteria

    Directory of Open Access Journals (Sweden)

    Nazmul Hossain

    2014-04-01

    Full Text Available In this study, attempts were made to isolate Streptomyces sp. from soil samples of two different regions of Bangladesh and evaluate their antagonistic activity against fish and human pathogenic bacteria. A total of 10 isolates were identified as Streptomyces sp. based on several morphological, physiological and biochemical tests. Cross streak method was used to observe the antagonistic activity of the Streptomyces sp. isolates against different fish pathogens belonging to the genus Aeromonas, Pseudomonas and Edwardsiella and human clinical isolates belonging to the genus Klebsiella, Salmonella and Streptococcus. Seven Streptomyces sp. isolates showed antagonism against both fish and human pathogenic bacteria. Four isolates viz., N24, N26, N28 and N47 showed broad spectrum of antagonistic activity (80-100% against all genera of fish and human pathogenic bacteria. The isolate N49 exhibited highest spectrum of antagonism against all fish pathogens (90-100% but comparatively lower degree of antagonism against human pathogens (50-60%. Rest of the two isolates (N21 and N23 showed variability in their antagonism. Results showed that broad spectrum antibiotic(s could be developed from the isolates N24, N26, N28 and N47against several human and fish pathogens. The isolate N49 could be a potential source of antibiotic, especially for fish pathogenic bacteria.

  10. DETEKSI KANDUNGAN RHODAMIN B PADA SAUS SERTA CEMARAN BORAKS DAN BAKTERI SALMONELLA SP. PADACILOK KELILING SALATIGA

    Directory of Open Access Journals (Sweden)

    Ardhikajaya Wahyu Prasetya

    2017-01-01

    Full Text Available ABSTRACT Borax and rhodamine B are preservative and dye banned to be used in food products. However, some traders still use borax and rhodamine B to their food and drink products. Cilok is a kind of food liked by schoolchildren and categorized as street vendor food (PKL. Most of food sold by street vendor has not touched by strict control from BPOM, so its quality is not good. Pentol cilok is a food made by starch. It tastes delicious and chewy. Furthermore, it is liked by schoolchildren. Cilok is also served by adding the sauce in order to make it tastier. It is common to add dye in that sauce and the dye is not natural dye nor food coloring. In the cilok, the contamination of microbe especially Salmonella sp. is influenced by unhygienic process. This research aims to find out borax and Salmonella sp. contamination and also the existence of rhodamine B on the cilok sauce. The methods used in this research are qualitative, quantitative, and the detection of Salmonella sp. contamination. The result shows, of the 8 tested samples, 4 samples (sample B, C, E, and H contain little amount of borax, the sauce contains no rhodamine B. This can be seen from Rf value and color reaction test. On the testing of Salmonella sp., there are bacteria before boiling process (when cilok is still in the form of dough and there are no bacteria after boiling and steaming process, so it is safe to be consumed.

  11. Prdx6 Upregulation by Curcumin Attenuates Ischemic Oxidative Damage via SP1 in Rats after Stroke

    Directory of Open Access Journals (Sweden)

    Gongwei Jia

    2017-01-01

    Full Text Available Background. The role of Peroxiredoxin 6 (Prdx6 in brain ischemia remains unclear. Curcumin (Cur treatment elicits neuroprotective effects against cerebral ischemic injury, and the associated mechanisms may involve Prdx6. In this study, we investigated whether Prdx6 and the transcription factor specific protein 1 (SP1 were involved in the antioxidant effect of Cur after stoke. Methods. Focal cerebral ischemic injury was induced by transient middle cerebral artery occlusion for 2 hours in male Sprague-Dawley rats treated with or without Prdx6 siRNA. Expression of Prdx6 in the penumbra was assessed by Real-Time PCR (RT-PCR, Western blot analysis, and immunoflourescent staining. In addition, infarct volume, neurological deficit score, and oxidative stress were evaluated. Prdx6 levels were also determined in the presence and absence of SP1 antagonist mithramycin A (MTM-A. Results. Cur treatment upregulated Prdx6 protein expression and the number of Prdx6-positive neuronal cells 24 hours after reperfusion. Cur treatment also attenuated oxidative stress and induced neuroprotective effects against ischemic damage, whereas the beneficial effects of Cur treatment were lost in animals treated with Prdx6-siRNA. Prdx6 upregulation by Cur treatment was abolished by SP1 antagonists MTM. Conclusions. Prdx6 upregulation by Cur treatment attenuates ischemic oxidative damage through SP1 induction in rats after stroke. This represents a novel mechanism of Cur-induced neuroprotection against cerebral ischemia.

  12. The effect of Pseudoxanthomonas sp. as manganese oxidizing bacterium on the corrosion behavior of carbon steel

    Energy Technology Data Exchange (ETDEWEB)

    Ashassi-Sorkhabi, H., E-mail: habib_ashassi@yahoo.com [Electrochemistry Research Laboratory, Physical Chemistry Department, Faculty of Chemistry, University of Tabriz, Tabriz (Iran, Islamic Republic of); Moradi-Haghighi, M. [Electrochemistry Research Laboratory, Physical Chemistry Department, Faculty of Chemistry, University of Tabriz, Tabriz (Iran, Islamic Republic of); Zarrini, G. [Microbiology laboratory, Biology Department, Science Faculty, University of Tabriz, Tabriz (Iran, Islamic Republic of)

    2012-02-01

    The present study investigated the role of manganese oxidizing bacterium (MOB), namely Pseudoxanthomonas sp. on the corrosion behavior of carbon steel. This bacterium was isolated from sewage treatment plants and identified by biochemical and molecular methods. The electrochemical techniques such as open circuit potentiometry, electrochemical impedance spectroscopy, potentiodynamic and cyclic polarization were used to measure the corrosion rate and observe the corrosion mechanism. Also, scanning electron microscopy and X-ray diffraction studies were applied to surface analysis. This study revealed the strong adhesion of the biofilm on the metal surface in the presence of Pseudoxanthomonas sp. that enhanced the corrosion of carbon steel. X-ray diffraction patterns identified a high content of MnO{sub 2} deposition within these biofilms. This is the first report that discloses the involvement of Pseudoxanthomonas sp. as manganese oxidizing bacteria on the corrosion of carbon steel. - Highlights: Black-Right-Pointing-Pointer A new type of manganese oxidizing bacteria, namely Pseudoxanthomonas sp. was indicated. Black-Right-Pointing-Pointer This bacterium can create a biofilm on the part of metal surface and affect localized corrosion. Black-Right-Pointing-Pointer In the presence of biofilm, the diffusion of oxygen vacancies and manganese ions has occurred.

  13. Larvacidal Effect of Imperata Cylindrical Root Decoction against Culex sp. Larvae

    Directory of Open Access Journals (Sweden)

    Afini Tiara Resi

    2014-08-01

    Full Text Available Background: Filariasis is one of the neglected tropical diseases. About 337 of 401 districts in Indonesia are endemic areas for filariasis, especially in Sumatera, Kalimantan, and Papua. Culex sp. is one of the lymphatic filariasis vectors which can be controlled by insecticide, including larvacide. This study was conducted to determine the larvacidal effect of Imperata cylindrical root decoction against Culex sp. larvae. Methods: This study was conducted at the Laboratory of Parasitology, Faculty of Medicine, Universitas Padjadjaran during the period of September to November 2012. The study object was Culex sp. larvae III/IV instars. The design of this study was experimental laboratory using true experimental approach. The larvae were divided into three groups: negative control (distilled water, reference (Abate®, and decoction. The number of larvae in each group was 25 larvae, and the effects were evaluated by the total number of dead larvae in 48 hours under observation. The data were then analyzed by Mann-Whitney test and Probit test. Results: The result of the Mann-Whitney test to compare Imperata cylindrica root decoction treatment to distilled water as control was significant (p<0.05. However, Abate® gave a better result. The Probit test resultwas LC50: 63% and LC90: 489%. Conclusions: Imperata cylindrical root decoction has a larvacidal effect against Culex sp. larvae.

  14. Cytotoxicity and Phytochemical Profiling of Sargassum Sp. Extract As Anti-Mdr Bacteria

    Science.gov (United States)

    Setyati, Wilis A.; Pramesti, Rini; Zainuddin, Muhamad; Puspita, Maya; Renta, Person P.

    2018-02-01

    Sargassum sp. contains bioactive compounds having the potential as an antibacterial agent. Sargassum sp. was collected from five different locations, i.e., Teluk Awur, Panjang Island, Bandengan, Ujung Piring, and Bondo. There were several different species of Sargassum sp. identified from each sampling locations. The collected seaweeds were washed, naturally dried, and ground to the powder-sized dry material. Dry seaweed was extracted gradually using n-hexane, ethyl acetate, and methanol (1:3 w/v). The antibacterial analysis was conducted based on Agar Diffusion method using Zobell as media. Resistance analysis was performed to evaluate the resistance of pathogen against commercial antibiotics, namely, chloramphenicol, ampicillin, erythromycin, amoxycillin, and tetracycline. Each Sargassum sp. extract was tested against three candidates of MDR bacteria, i.e., Staphylococcus aureus, Escherichia coli, and S. epidermidis. Results showed that S. aureus was resistant towards four out of five commercial antibiotics. E. coli and S. epidermidis were not susceptible to two and three out of five commercial antibiotics, respectively. N-hexane, ethyl acetate and methanol yielded 0.1-0.3%, 0.3-0.7 % and 0.8-4.7% of dry extract. Ethyl acetate extract of Sargassum from Teluk Awur performed the best antibacterial activity and contained an alkaloid, flavonoid, and phenolic compounds. Toxicity analysis showed that this ethyl acetate extract had LC50 at 463 ppm and categorized as chronic toxicity.

  15. Biodegradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1

    Directory of Open Access Journals (Sweden)

    Preeti N. Tallur

    2015-09-01

    Full Text Available Pyrethroid pesticide cypermethrin is a environmental pollutant because of its widespread use, toxicity and persistence. Biodegradation of such chemicals by microorganisms may provide an cost-effective method for their detoxification. We have investigated the degradation of cypermethrin by immobilized cells of Micrococcus sp. strain CPN 1 in various matrices such as, polyurethane foam (PUF, polyacrylamide, sodium alginate and agar. The optimum temperature and pH for the degradation of cypermethrin by immobilized cells of Micrococcus sp. were found to be 30 °C and 7.0, respectively. The rate of degradation of 10 and 20 mM of cypermethrin by freely suspended cells were compared with that of immobilized cells in batches and semi-continuous with shaken cultures. PUF-immobilized cells showed higher degradation of cypermethrin (10 mM and 20 mM than freely suspended cells and cells immobilized in other matrices. The PUF-immobilized cells of Micrococcus sp. strain CPN 1 were retain their degradation capacity. Thus, they can be reused for more than 32 cycles, without losing their degradation capacity. Hence, the PUF-immobilized cells of Micrococcus sp. could potentially be used in the bioremediation of cypermethrin contaminated water.

  16. Ocorrência de Taenia sp. na população atendida no laboratório central do Instituto Adolfo Lutz, São Paulo, SP, Brasil (1960/1989 Occurrence of Taenia sp. in the population attended in the central laboratory of "Instituto Adolfo Lutz", São Paulo, SP, Brazil (1960/1989

    Directory of Open Access Journals (Sweden)

    Rosa Maria Donini Souza Dias

    1991-04-01

    Full Text Available Foram examinados retrospectivamente os relatórios mensais e anuais da Seção de Enteroparasitoses do Laboratório Central do Instituto Adolfo Lutz, São Paulo, SP, do período de 1960 a 1989, perfazendo uma série histórica de 30 anos, com 1.519.730 exames protoparasitológicos e 355 identificações de proglotes de Taenia. Pelo método da sedimentação espontânea foram diagnosticados 7.663 (0,5% casos de presença de ovos de Taenia sp. nas fezes. Das 355 proglotes enviadas para identificação, 311 (87,60% estavam em condições de serem especificadas, e dessas, 273 (87,80% eram proglotes de Taenia saginata e 38 (12,22% de T. solium.Monthly and yearly reports of the Seção de Enteroparasitoses of the Instituto Adolfo Lutz (São Paulo, SP, Brazil from 1960 to 1989 with 1,519,730 parasitological stool examinations were studied. There were also 355 identifications of Taenia sp. proglottids. Using HOFFMAN, PONS & JANER's method, 7,663 (0.5% cases of taeniasis were diagnosed, and 311 (87.60% of the 355 proglottids were on easy terms to be specified, 273 (87.80% of them were from Taenia saginata.

  17. The influence of supplemented Curcuma in feed formulation to improve growth rate and feed efficiency of catfish (Clarias sp.)

    Science.gov (United States)

    Ulum, M. M.; Zubaidah, M.; Arief, M.; Prayogo

    2018-04-01

    Catfish (Clarias sp.) is very potential to be developed as a food fish. The use of the feed additive in feed intended to improve the health, productivity and compliance with animal nutrition. Feed additive is to use ginger are derived from nature.The purpose of this study was to determine the potential increase in feed formulation. The Curcuma effect on growth rate and feed efficiency of catfish (Clarias sp.). The method used experimental methods and design complete random with 4 treatments and 5 replications. The parameters examined in this study aregrowth rate and efficiency feed.The research showed that the ANOVA markedly dissimilar ( Padditive on formulations feed.

  18. Rhodotorula rosulata sp. nov., Rhodotorula silvestris sp. nov. and Rhodotorula straminea sp. nov., novel myo-inositol-assimilating yeast species in the Microbotryomycetes.

    Science.gov (United States)

    Golubev, Wladyslav I; Scorzetti, Gloria

    2010-10-01

    Three novel species are described as Rhodotorula rosulata sp. nov. (type strain VKM Y-2962(T) =CBS 10977(T)), Rhodotorula silvestris sp. nov. (type strain VKM Y-2971(T) =CBS 11420(T)) and Rhodotorula straminea sp. nov. (type strain VKM Y-2964(T) =CBS 10976(T)) based on the study of eight isolates from needle litter. The new species, phylogenetically located within the Microbotryomycetes, are related to glucuronate-assimilating species of the genus Rhodotorula. Sequencing of the D1/D2 domains of the LSU rDNA gene and the internal transcribed spacer (ITS) region, as well as physiological characterization, revealed their distinct taxonomic positions.

  19. Kroyeria deetsi n.sp. (Kroyeriidae: Siphonostomatoida), a parasitic ...

    African Journals Online (AJOL)

    Kroyeria deetsi n.sp. (Kroyeriidae: Siphonostomatoida) is described from both sexes collected from the gills of spinner sharks, Carcharhinus brevipinna (Müller & Henle, 1839), captured in the Indian Ocean off the coast of South Africa. Kroyeria deetsi n.sp. can easily be distinguished from all of its congeners because the ...

  20. HANSENULA WICKERHAMII SP. N., A NEW YEAST FROM FINNISH SOIL

    Science.gov (United States)

    Capriotti, Augusto

    1961-01-01

    Capriotti, Augusto (l'Università di Perugia, Perugia, Italy). Hansenula wickerhamii sp. n., a new yeast from Finnish soil. J. Bacteriol. 82:259–360. 1961.—Hansenula wickerhamii sp. n. is described; it was isolated from a Finnish soil, and is named in honor of Lynferd J. Wickerham. Images PMID:13690638

  1. Multidisciplinary study (CO2 flux, ERT, self-potential, permeability and structural surveys) in Fondi di Baia, Astroni and Agnano volcanoes: insights for the structural architecture of the Campi Flegrei caldera (southern Italy)

    Science.gov (United States)

    Isaia, Roberto; Carapezza, Maria Luisa; Conti, Eric; Giulia Di Giuseppe, Maria; Lucchetti, Carlo; Prinzi, Ernesto; Ranaldi, Massimo; Tarchini, Luca; Tramparulo, Francesco; Troiano, Antonio; Vitale, Stefano; Cascella, Enrico; Castello, Nicola; Cicatiello, Alessandro; Maiolino, Marco; Puzio, Domenico; Tazza, Lucia; Villani, Roberto

    2017-04-01

    Recent volcanism at Campi Flegrei caldera produced more than 70 eruptions in the last 15 ka formed different volcanic edifices. The vent distribution was related to the main volcano-tectonic structure active in the caldera along which also concentrated part of the present hydrothermal and fumarolic activity, such as in the Solfatara area. In order to define the role of major faults in the Campi Flegrei Caldera, we analyzed some volcanic craters (Fondi di Baia and Astroni) and the Agnano caldera, by means of different geochemical and geophysical technics including CO2 flux, electrical resistivity (ERT), self-potential and permeability surveys. We provided some ERT profiles and different maps of geochemical and geophysical features. Major fault planes were identified comparing ERT imaging with alignments of anomalies in maps. The results can improve the knowledge on the present state of these volcanoes actually not fully monitored though included in the area with high probability of future vent opening within the Campi Flegrei caldera.

  2. Evaluation of FASP, SP3, and iST Protocols for Proteomic Sample Preparation in the Low Microgram Range.

    Science.gov (United States)

    Sielaff, Malte; Kuharev, Jörg; Bohn, Toszka; Hahlbrock, Jennifer; Bopp, Tobias; Tenzer, Stefan; Distler, Ute

    2017-11-03

    Efficient and reproducible sample preparation is a prerequisite for any robust and sensitive quantitative bottom-up proteomics workflow. Here, we performed an independent comparison between single-pot solid-phase-enhanced sample preparation (SP3), filter-aided sample preparation (FASP), and a commercial kit based on the in-StageTip (iST) method. We assessed their performance for the processing of proteomic samples in the low μg range using varying amounts of HeLa cell lysate (1-20 μg of total protein). All three workflows showed similar performances for 20 μg of starting material. When handling sample sizes below 10 μg, the number of identified proteins and peptides as well as the quantitative reproducibility and precision drastically dropped in case of FASP. In contrast, SP3 and iST provided high proteome coverage even in the low μg range. Even when digesting 1 μg of starting material, both methods still enabled the identification of over 3000 proteins and between 25 000 and 30 000 peptides. On average, the quantitative reproducibility between experimental replicates was slightly higher in case of SP3 (R 2 = 0.97 (SP3); R 2 = 0.93 (iST)). Applying SP3 toward the characterization of the proteome of FACS-sorted tumor-associated macrophages in the B16 tumor model enabled the quantification of 2965 proteins and revealed a "mixed" M1/M2 phenotype.

  3. Identification of Glomerella cingulata f. sp phaseoli recombinants by RAPD markers.

    Science.gov (United States)

    Camargo, O A; Souza, E A; Mendes-Costa, M C; Santos, J B; Soares, M A

    2007-09-30

    We examined the capacity of strains of Glomerella cingulata f. sp phaseoli fungus (Colletotrichum lindemuthianum sexual stage) to form recombinants, using random amplified polymorphic DNA (RAPD). Crosses of all possible combinations between strains 40, 42, 20, 21, 22, 23, 24, 25, and 26 were made on Petri dishes using M3 culture medium. The 42 x 21 cross produced the largest number of perithecia and five asci; the respective ascospores were isolated. RAPD analysis was performed on the parents and descendants. The 62 polymorphic RAPD bands obtained were used to assess the genetic similarity using the method of Sorence and Dice and clustering analysis in the form of a dendrogram by the UPGMA method. The RAPD markers allowed identification of recombinants from the cross between strains 42 and 21 of G. cingulata f. sp phaseoli and 40 ascospores presented 63 and 49% genetic similarity with parents 2 (strain 42) and 1 (strain 21), respectively.

  4. Symbiotic Fungus of Marine Sponge Axinella sp. Producing Antibacterial Agent

    Science.gov (United States)

    Trianto, A.; Widyaningsih, S.; Radjasa, OK; Pribadi, R.

    2017-02-01

    The emerging of multidrug resistance pathogenic bacteria cause the treatment of the diseaseshave become ineffective. There for, invention of a new drug with novel mode of action is an essential for curing the disease caused by an MDR pathogen. Marine fungi is prolific source of bioactive compound that has not been well explored. This study aim to obtain the marine sponges-associated fungus that producing anti-MDR bacteria substaces. We collected the sponge from Riung water, NTT, Indonesia. The fungus was isolated with affixed method, followed with purification with streak method. The overlay and disk diffusion agar methods were applied for bioactivity test for the isolate and the extract, respectively. Molecular analysis was employed for identification of the isolate. The sponge was identified based on morphological and spicular analysis. The ovelay test showed that the isolate KN15-3 active against the MDR Staphylococcus aureus and Eschericia coli. The extract of the cultured KN15-3 was also inhibited the S. aureus and E. coli with inhibition zone 2.95 mm and 4.13 mm, respectively. Based on the molecular analysis, the fungus was identified as Aspergillus sydowii. While the sponge was identified as Axinella sp.

  5. Solar drying of rose (Rosa sp.) petals

    Energy Technology Data Exchange (ETDEWEB)

    Balladin, D.A.; Headley, Oliver [University of the West Indies, Center for Resource Management and Environmental Studies, St. Michael (Barbados)

    1999-10-01

    The rose (Rosa sp.) petals can be dried after 2 days at about 30degC reaching an equilibrium moisture content after 16 h using the solar wire basket dryer. The initial moisture content (wet wt basis) and final moisture content (dry wt basis), determined by the Dean-Stark toluene were 65.7 and 25.2% respectively. The intensity of the rose red coloured pigment (pelargonidin) decreased by a factor of 2.5 after drying. The pelargonidin ethanoic extract as an acid-base indicator, has a K{sub 4} value of 1 x 10{sup -4} mol 1{sup -1} and pH of end point 4 and imbibed on filter paper and allowed to air dry for 5 min showed excellent properties as acid-based test tapes. (Author)

  6. Electron microscope study of Sarcocystis sp

    Science.gov (United States)

    Zeve, V.H.; Price, D.L.; Herman, C.M.

    1966-01-01

    Sarcocystis sp. obtained from wild populations of grackles, Quiscalus quiscula (Linn.), were examined to clarify the effect of the parasite on the host. Electron micrographs are presented to show areas of muscle destruction adjacent to the parasite which appear to be mechanically produced by the parasite. The microtubules within the villus-like projections of the cyst suggest that their possible function is absorptive and/or conductive with regard to the production of a toxin or the conveyance of nutritive material to the developing cells. The proposed function of submembranous filaments and their relation to the conoid is discussed. Similarities in the ultrastructure to Toxoplasma and other protozoa tend to negate the relegation of Sarcocystis to the fungi and further emphasize its protozoan nature.

  7. Polyhydroxybutyrate accumulation by a Serratia sp.

    Science.gov (United States)

    Lugg, Harriet; Sammons, Rachel L; Marquis, Peter M; Hewitt, Christopher J; Yong, Ping; Paterson-Beedle, Marion; Redwood, Mark D; Stamboulis, Artemis; Kashani, Mitra; Jenkins, Mike; Macaskie, Lynne E

    2008-03-01

    A strain of Serratia sp. showed intracellular electron-transparent inclusion bodies when incubated in the presence of citrate and glycerol 2-phosphate without nitrogen source following pre-growth under carbon-limitation in continuous culture. About 1.3 mmol citrate were consumed per 450 mg biomass, giving a calculated yield of maximally 55% of stored material per g of biomass dry wt. The inclusion bodies were stained with Sudan Black and Nile Red (NR), suggesting a lipid material, which was confirmed as polyhydroxybutyrate (PHB) by analysis of molecular fragments by GC and by FTIR spectroscopy of isolated bio-PHB in comparison with reference material. Multi-parameter flow cytometry in conjunction with NR fluorescence, and electron microscopy, showed that not all cells contained heavy PHB bodies, suggesting the potential for increasing the overall yield. The economic attractiveness is enhanced by the co-production of nanoscale hydroxyapatite (HA), a possible high-value precursor for bone replacement materials.

  8. In vitro rootting of Dioscoreas sp.

    Directory of Open Access Journals (Sweden)

    Irma Quintero

    2003-07-01

    Full Text Available The Universidad de Córdoba s Vegetal Tissue Culture Laboratory evaluated the effect of naphthalenacetic acid (NAA on in vitro rooting of three yam cultivars (Dioscorea sp. in 2000. The effect of four hormone levels (0, 0.3, 0.6 and 0.9 mg/1 was studied on three yam cultivars (Diamantes-22,003 and 005. A random experimental design was used employing 4x3 factorial arrangement and 20 repetitions; each experimental unit consisted of a glass receptacle containing the culture medium and the explant (one segment nodal. The variables considered were the number of roots and their thickness, culture medium oxidation and callus production. Findings showed that both the hormone and genotype had an effect on all those variables considered in the study and interaction was significant (P

  9. Aspergillus waksmanii sp. nov. and Aspergillus marvanovae sp. nov., two closely related species in section Fumigati

    DEFF Research Database (Denmark)

    Hubka, Vit; Peterson, Stephen W.; Frisvad, Jens Christian

    2013-01-01

    Two new and phylogenetically closely related species in Aspergillus section Fumigati are described and illustrated. Homothallic Aspergillus waksmanii sp. nov. was isolated from New Jersey soil (USA) and is represented by the ex-type isolate NRRL 179T (=CCF 4266T=Thom 4138.HS2T=IBT 31900T......). Aspergillus marvanovae sp. nov. was isolated from water with high boracic acid anions content in Dukovany nuclear power station (Czech Republic). The sexual stage of this species is unknown, but the MAT1-1 locus was successfully amplified suggesting that the species is probably heterothallic and teleomorphic...... but is represented by only the ex-type isolate CCM 8003T (=CCF 4037T=NRRL 62486T=IBT 31279T=IFM 60873T). Both species can be distinguished from all previously described species in section Fumigati based on morphology, maximum growth temperature, sequence data from five unlinked loci and unique secondary metabolites...

  10. Six New Polyacetylenic Alcohols from the Marine Sponges Petrosia sp. and Halichondria sp.

    Science.gov (United States)

    Gabriel, Adeyemi Francis; Li, Zhen; Kusuda, Ryouhei; Tanaka, Chiaki; Miyamoto, Tomofumi

    2015-01-01

    Six new polyacetylenic alcohols, termed strongylotriols A and B; pellynols J, K, and L; and isopellynol A, together with three known polyacetylenic alcohols, pellynols A, B, and C were isolated from the marine sponges Petrosia sp., and Halichondria sp. collected in Okinawa, Japan. Their planer structures were determined based on 2D-NMR and mass spectrometric analysis of the degraded products by RuCl3 oxidation. The absolute stereochemistry of isolates was examined by their Mosher's esters. The strongylotriols were found to be optically pure compounds, whereas the pellynols are diastereomeric mixtures at the C-6 position. Proliferation experiments using the HeLa and K562 cell lines suggested that the essential structural units for activity are the "hexa-2,4-diyn-1,6-diol" and "pent-1-en-4-yn-3-ol" on the termini.

  11. Kinerja probiotik Bacillus sp. pada pendederan benih ikan lele Clarias sp. yang diinfeksi Aeromonas hydrophila

    Directory of Open Access Journals (Sweden)

    , Sukenda

    2016-12-01

    Full Text Available ABSTRACT This experiment was conducted to assess performance of Bacillus sp. probiotic on catfish juvenile Clarias sp. infected by Aeromonas hydrophila. The probiotic content in the diets were 0% (K+ and K-, 1%, and 2% in duplicates. This experiment used randomized design with four treatments and two replications. Juveniles with average body weight of 3.22±0.15 g/fish were reared in the 1.5×2.8×0.5 m3 pond with density of 800 fish/pond. Fish were reared for 30 days and fed three times a day at rate 8% of  total body weight. At day 31, catfish were challenged by A. hydrophila 0.1 mL (106 cfu/mL. Post infection observation was carried out ten days with density 10 fish/aquaria. The result showed that fish fed diet containing 2% probiotic gave the best probiotic performance with survival rate of catfish 83.33% after challenged, spesific growth rate 5.40%, and 0,75 of feed conversion ratio. The results of the blood profile showed significantly better results in the treatment of probiotics compared to the positive control after challenge test A. hydrophila. Probiotic Bacillus sp. has given as much as 2% on feed provides better performance on catfish juvenile. Keywords: probiotic, Bacillus sp., A. hydrophila, catfish juvenille, growth  ABSTRAK Penelitian ini bertujuan untuk menguji kinerja probiotik Bacillus sp. dalam pakan pada pendederan benih ikan lele Clarias sp. yang diinfeksi bakteri Aeromonas hydrophila. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan yaitu kandungan probiotik dalam pakan perlakuan yaitu 0% (K+ dan K-, 1%,  dan 2%, masing-masing dengan dua ulangan. Ikan lele yang digunakan memiliki bobot rata-rata 3,22±0,15 g/ekor, dipelihara dalam kolam terpal berukuran 1,5×2,8×0,5 m3 dengan kepadatan 800 ekor/kolam. Ikan dipelihara selama 30 hari dengan frekuensi pemberian pakan tiga kali sehari sebanyak 8% dari bobot tubuh ikan. Hari ke-31 benih lele diinjeksi bakteri A. hydrophila dosis 0,1 m

  12. NORIA-SP: A finite element computer program for analyzing liquid water transport in porous media

    International Nuclear Information System (INIS)

    Hopkins, P.L.; Eaton, R.R.; Bixler, N.E.

    1991-12-01

    A family of finite element computer programs has been developed at Sandia National Laboratories (SNL) most recently, NORIA-SP. The original NORIA code solves a total of four transport equations simultaneously: liquid water, water vapor, air, and energy. Consequently, use of NORIA is computer-intensive. Since many of the applications for which NORIA is used are isothermal, we decided to ''strip'' the original four-equation version, leaving only the liquid water equation. This single-phase version is NORIA-SP. The primary intent of this document is to provide the user of NORIA-SP an accurate user's manual. Consequently, the reader should refer to the NORIA manual if additional detail is required regarding the equation development and finite element methods used. The single-equation version of the NORIA code (NORIA-SP) has been used most frequently for analyzing various hydrological scenarios for the potential underground nuclear waste repository at Yucca Mountain in western Nevada. These analyses are generally performed assuming a composite model to represent the fractured geologic media. In this model the material characteristics of the matrix and the fractures are area weighted to obtain equivalent material properties. Pressure equilibrium between the matrix and fractures is assumed so a single conservation equation can be solved. NORIA-SP is structured to accommodate the composite model. The equations for water velocities in both the rock matrix and the fractures are presented. To use the code for problems involving a single, nonfractured porous material, the user can simply set the area of the fractures to zero

  13. Descriptions of Deladenus albizicus n. sp. and D. processus n. sp. (Nematoda: Hexatylina) from Haryana, India.

    Science.gov (United States)

    Tomar, V V S; Somvanshi, Vishal S; Bajaj, Harish K

    2015-03-01

    Two different nematodes were isolated from the bark of Albizia lebbeck trees; one from insect infested and another from noninfested, healthy tree. Based on the biological, morphological, and molecular evidences, the nematodes are described as Deladenus albizicus n. sp. and D. processus n. sp. (Nematoda: Hexatylina). Deladenus albizicus n. sp., isolated from insect-infested tree, multiplied on the fungus Nigrospora oryzae. Myceliophagous females of this nematode reproduced by parthenogenesis and spermathecae were indistinct. Infective females, readily produced in the cultures, are dorsally curved. Only one type of males containing small-sized sperms in their genital tracts were produced in the culture. Myceliophagous females: L = 0.75 to 1.71 mm, a = 32.3 to 50.8, b = 9.3 to 11.2, b' = 5.2 to 7.3, c = 27.2 to 35.6, V = 91.0 to 93.3, c' = 2.0 to 2.9, stylet = 11 to 12 µm, excretory pore in the region of median pharyngeal bulb, 43 to 47 µm anterior to hemizonid. Deladenus processus n. sp., isolated from bark of healthy A. lebbeck tree, was cultured on Alternaria alternata. Myceliophagous females reproduced by amphimixis and their spermathecae contained rounded sperms. Infective females were never produced, even in old cultures. Myceliophagous females: L = 0.76 to 0.99 mm, a = 34 to 49, b = 13.3 to 17.7, b' = 3.8 to 5.8, c = 19.6 to 22.8, V = 92.2 to 93.5, c' = 2.7 to 3.5, stylet = 6 to 7 µm, excretory pore in the proximity of hemizonid, tail conoid, tapering from both sides to a long pointed central process. It is proposed to classify Deladenus species in three groups: durus, siricidicola, and laricis groups based on female and spermatogonia dimorphism, mode of reproduction, and insect parasitism.

  14. Optimization of Protease Production by Psychrotrophic Rheinheimera sp. with Response Surface Methodology

    OpenAIRE

    Mrayam Mahjoubin-Tehran; Bahar Shahnavaz; Razie Ghazi-Birjandi; Mansour Mashreghi; Jamshid Fooladi

    2016-01-01

    Background and Objectives: Psychrotrophic bacteria can produce enzymes at low temperatures; this provides a wide biotechnological potential, and offers numerous economical advantages over the use of mesophilic bacteria. In this study, extracellular protease production by psychrotrophic Rheinheimera sp. (KM459533) was optimized by the response surface methodology.Materials and Methods: The culture medium was tryptic soy broth containing 1% (w v -1 ) skim milk. First, the effects of variables w...

  15. Uji Daya Hambat Ekstrak Rimpang Kunyit (Curcuma Longa) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Dan Pseudomonas SP.

    OpenAIRE

    Pangemanan, Andrew; Fatimawali; Budiarso, Fona

    2016-01-01

    : Turmeric (Curcuma longa) is a plant that is known to have medicinal properties, especially the rhizome. The active compound that contained in the rhizomes are able to work as an antibacterial. This study aimed to measure the inhibitory of turmeric rhizome (Curcuma longa) extract against the growth of Staphylococcus aureus and Pseudomonas sp. This was an experimental laboratory study. The polar extract of turmeric rhizome (Curcuma longa) was tested by well method with concentration of 40%, 2...

  16. A Multi-Scale Computational Study on the Mechanism of Streptococcus pneumoniae Nicotinamidase (SpNic)

    OpenAIRE

    Ion, Bogdan; Kazim, Erum; Gauld, James

    2014-01-01

    Nicotinamidase (Nic) is a key zinc-dependent enzyme in NAD metabolism that catalyzes the hydrolysis of nicotinamide to give nicotinic acid. A multi-scale computational approach has been used to investigate the catalytic mechanism, substrate binding and roles of active site residues of Nic from Streptococcus pneumoniae (SpNic). In particular, density functional theory (DFT), molecular dynamics (MD) and ONIOM quantum mechanics/molecular mechanics (QM/MM) methods have been employed. The o...

  17. Successful treatment of an invasive fungal infection caused by Talaromyces sp. with voriconazole

    Directory of Open Access Journals (Sweden)

    Uluhan Sili

    2015-06-01

    Full Text Available Invasive fungal infections (IFI are on the rise due to increasing numbers of immunosuppressed and critically ill patients. A malignant-looking pulmonary nodule in an immunosuppressed patient may indeed be caused by a fungal organism. We report a patient, who was eventually diagnosed with an IFI caused by an agent of hyalohyphomycosis, Talaromyces sp. determined via molecular methods and succesfully treated with voriconazole.

  18. Handling and disposal of SP-100 ground test nuclear fuel and equipment

    International Nuclear Information System (INIS)

    Wilson, C.E.; Potter, J.D.; Hodgson, R.D.

    1990-05-01

    The post SP-100 reactor testing period will focus on defueling the reactor, packaging the various radioactive waste forms, and shipping this material to the appropriate locations. Remote-handling techniques will be developed to defuel the reactor. Packaging the spent fuel and activated reactor components is a challenge in itself. This paper presents an overview of the strategy, methods, and equipment that will be used during the closeout phase of nuclear testing

  19. Handling and disposal of SP-100 ground test nuclear fuel and equipment

    International Nuclear Information System (INIS)

    Wilson, C.E.; Potter, J.D.; Hodgson, R.D.

    1991-01-01

    The post SP-100 reactor testing period will focus on defueling the reactor, packaging the various radiactive waste forms, and shipping this material to the appropriate locations. Remote-handling techniques will be developed to defuel the reactor. Packaging the spent fuel and activated reactor components is a challenge in itself. This paper presents an overview of the strategy, methods, and equipment that will be used during the closeout phase of nuclear testing

  20. Synthesis of chiral phosphonoacetamides and their toxic effects on paramecium sp

    Directory of Open Access Journals (Sweden)

    Samia Guezane Lakoud

    2015-02-01

    Full Text Available Three chiral phosphonoacetamides were prepared by an alternative method . For this purpose, 2-(diethoxyphosphorylacetic acid was prepared from ethyl 2-bromoacetate by treatment of P(OEt 3 followed by saponification of the ester with K 2CO 3. BOP activated amidation of the 2-(diethoxyphosphorylacetic acid with (S-amino acids gave the corresponding phosphonoaceteamides. Growth inhibition of two phosphonoacetamides on Paramecium sp. were studied.

  1. Cladielloides A and B: New Eunicellin-Type Diterpenoids from an Indonesian Octocoral Cladiella sp

    Science.gov (United States)

    Chen, Yung-Husan; Tai, Chia-Ying; Hwang, Tsong-Long; Weng, Ching-Feng; Li, Jan-Jung; Fang, Lee-Shing; Wang, Wei-Hsien; Wu, Yang-Chang; Sung, Ping-Jyun

    2010-01-01

    Two new eunicellin-type diterpenoids, cladielloides A (1) and B (2), which were found to possess a 2-hydroxybutyroxy group in their structures, were isolated from an Indonesian octocoral identified as Cladiella sp. The structures of eunicellins 1 and 2 were elucidated by spectroscopic methods. Cladielloide B (2) exhibited moderate cytotoxicity toward CCRF-CEM tumor cells and this compound displayed significant inhibitory effects on superoxide anion generation and elastase release by human neutrophils. PMID:21339957

  2. Haenamindole, an unusual diketopiperazine derivative from a marine-derived Penicillium sp. KCB12F005.

    Science.gov (United States)

    Kim, Jong Won; Ko, Sung-Kyun; Son, Sangkeun; Shin, Kee-Sun; Ryoo, In-Ja; Hong, Young-Soo; Oh, Hyuncheol; Hwang, Bang Yeon; Hirota, Hiroshi; Takahashi, Shunji; Kim, Bo Yeon; Osada, Hiroyuki; Jang, Jae-Hyuk; Ahn, Jong Seog

    2015-11-15

    During the chemical investigation of marine-derived fungus, an unusual diketopiperazine (DKP) alkaloid, haenamindole (1), was isolated from a culture of the marine-derived fungus Penicillium sp. KCB12F005. The structure of 1, which possesses benzyl-hydroxypiperazindione and phenyl-pyrimidoindole rings system in the molecule, was elucidated by analysis of NMR and MS data. The stereochemistry of 1 was determined by ROESY and advanced Marfey's method. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Burrowing by Sailfin Catfish (Pterygoplichthys sp.): A Potential Cause of Erosion in Disturbed Environments

    Science.gov (United States)

    2014-03-01

    originates at a large levee, which was built to create a water retention (to control excess water) and water bird management area. The levee and canal...each sample was dried in stainless steel trays in a 60 °C convection oven . Two methods of quantitative analysis were performed to determine the...ERDC/TN ANSRP-14-1 March 2014 Approved for public release; distribution is unlimited. Burrowing by Sailfin Catfish (Pterygoplichthys sp.): A

  4. Pd(II)-Catalyzed Olefination of sp3 C–H Bonds

    Science.gov (United States)

    Wasa, Masayuki; Engle, Keary M.; Yu, Jin-Quan

    2010-01-01

    The first Pd(II)-catalyzed sp3 C–H olefination reaction has been developed using N-arylamide directing groups. Following olefination, the resulting intermediates were found to undergo rapid 1,4-addition to give the corresponding γ lactams. Notably, this method was effective with substrates containing α-hydrogen atoms and could be applied to effect methylene C–H olefination of cyclopropane substrates. PMID:20187642

  5. Endosulfan induced alteration in bacterial protein profile and RNA yield of Klebsiella sp. M3, Achromobacter sp. M6, and Rhodococcus sp. M2.

    Science.gov (United States)

    Singh, Madhu; Singh, Dileep Kumar

    2014-01-30

    Three bacterial strains identified as Klebsiella sp. M3, Achromobacter sp. M6 and Rhodococcus sp. M2 were isolated by soil enrichment with endosulfan followed by shake flask enrichment technique. They were efficiently degrading endosulfan in the NSM (non sulfur medium) broth. Degradation of endosulfan was faster with the cell free extract of bacterial cells grown in the sulfur deficient medium (NSM) supplemented with endosulfan than that of nutrient rich medium (Luria Bertani). In the cell free extract of NSM supplemented with endosulfan as sole sulfur source, a unique band was visualized on SDS-PAGE but not with magnesium sulfate as the sole sulfur source in NSM and LB with endosulfan. Expression of a unique polypeptide band was speculated to be induced by endosulfan under sulfur starved condition. These unique polypeptide bands were identified as OmpK35 protein, sulfate binding protein and outer membrane porin protein, respectively, in Klebsiella sp. M3, Achromobacter sp. M6 and Rhodococcus sp. M2. Endosulfan showed dose dependent negative effect on total RNA yield of bacterial strains in nutrient rich medium. Absence of plasmid DNA indicated the presence of endosulfan metabolizing gene on genomic DNA. Copyright © 2013 Elsevier B.V. All rights reserved.

  6. Wnt-mediated down-regulation of Sp1 target genes by a transcriptional repressor Sp5

    Czech Academy of Sciences Publication Activity Database

    Fujimura, Naoko; Vacík, Tomáš; Machoň, Ondřej; Vlček, Čestmír; Scalabrin, S.; Speth, M.; Diep, D.; Krauss, S.; Kozmik, Zbyněk

    2007-01-01

    Roč. 282, č. 2 (2007), s. 1225-1237 ISSN 0021-9258 Institutional research plan: CEZ:AV0Z50520514 Keywords : Wnt -mediated signaling * Sp5 transcription factor * Sp1 target genes Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 5.581, year: 2007

  7. Structural Analysis of Planar sp3 and sp2 Films: Diamond-Like Carbon and Graphene Overlayers

    KAUST Repository

    Mansour, Ahmed

    2011-01-01

    pure sp2 as in graphene, carbon nanotubes and fullerenes, to pure sp3 as in diamond. Amorphous and diamond-like carbon consists of a mixture of both hybridizations. This variation in hybridization in carbon materials enables a wide spectrum

  8. Formulation of a minimal nutritional medium for enhanced lipid productivity in Chlorella sp. and Botryococcus sp. using response surface methodology.

    Science.gov (United States)

    Vishwakarma, Rashi; Dhar, Dolly Wattal; Pabbi, Sunil

    2018-03-01

    Chlorella sp. MCC 7 and Botryococcus sp. MCC 31 were investigated to enable large-scale biodiesel production from minimal constituents in the growth medium. Response surface methodology (RSM) was used to maximise the biomass productivity and lipid yield using only nitrogen (N), phosphorus (P) and potassium (K) as urea, single super phosphate and muriate of potash. The optimum values were 0.42 g/L nitrogen; 0.14 g/L phosphorus and 0.22 g/L potassium for Chlorella sp.; and 0.46 g/L; 0.14 g/L and 0.25 g/L for Botryococcus sp. Lipid yield of 42% for Chlorella sp. and 52% in Botryococcus sp. was observed. An enhancement in lipid yield by approximately 55% for Chlorella sp. and 73% for Botryococcus sp. was registered as compared to original nutrient medium. Fourier transform infrared (FTIR) analysis of extracted lipids revealed characteristic bands for triglycerides. This study provided utilisation of a practicable nutrient recipe in the form of N, P, K input for enhanced lipid yield from the selected microalgal strains.

  9. Elevated expression and potential roles of human Sp5, a member of Sp transcription factor family, in human cancers

    International Nuclear Information System (INIS)

    Chen Yongxin; Guo Yingqiu; Ge Xijin; Itoh, Hirotaka; Watanabe, Akira; Fujiwara, Takeshi; Kodama, Tatsuhiko; Aburatani, Hiroyuki

    2006-01-01

    In this report, we describe the expression and function of human Sp5, a member of the Sp family of zinc finger transcription factors. Like other family members, the Sp5 protein contains a Cys2His2 zinc finger DNA binding domain at the C-terminus. Our experiments employing Gal4-Sp5 fusion proteins reveal multiple transcriptional domains, including a N-terminal activity domain, an intrinsic repressive element, and a C-terminal synergistic domain. Elevated expression of Sp5 was noted in several human tumors including hepatocellular carcinoma, gastric cancer, and colon cancer. To study the effects of the Sp5 protein on growth properties of human cancer cells and facilitate the identification of its downstream genes, we combined an inducible gene expression system with microarray analysis to screen for its transcriptional targets. Transfer of Sp5 into MCF-7 cells that expressed no detectable endogenous Sp5 protein elicited significant growth promotion activity. Several of the constitutively deregulated genes have been associated with tumorigenesis (CDC25C, CEACAM6, TMPRSS2, XBP1, MYBL1, ABHD2, and CXCL12) and Wnt/β-Catenin signaling pathways (BAMBI, SIX1, IGFBP5, AES, and p21 WAF1 ). This information could be utilized for further mechanistic research and for devising optimized therapeutic strategies against human cancers

  10. The human luteinizing hormone receptor gene promoter: activation by Sp1 and Sp3 and inhibitory regulation.

    Science.gov (United States)

    Geng, Y; Tsai-Morris, C H; Zhang, Y; Dufau, M L

    1999-09-24

    To understand the transcriptional mechanism(s) of human LH receptor (LHR) gene expression, we have identified the dominant functional cis-elements that regulate the activity of the promoter domain (-1 to -176 bp from ATG). Mutagenesis demonstrated that the promoter activity was dependent on two Sp1 domains (-79 bp, -120 bp) in a transformed normal placental cell (PLC) and the choriocarcinoma JAR cell. Both elements interacted with endogenous Sp1 and Sp3 factors but not with Sp2 or Sp4. In Drosophila SL2 cells, the promoter was activated by either Sp1 or Sp3. An ERE half-site (EREhs) at -174 bp was inhibitory (by 100%), but was unresponsive to estradiol and did not bind the estrogen receptor or orphan receptors ERR1 and SF-1. The 5' upstream sequence (-177 to -2056 bp) inhibited promoter activity in PLC by 60%, but only minimally in JAR cells. Activation of the human LHR promoter through Sp1/3 factors is negatively regulated through EREhs and upstream sequences to exert control of gene expression. Copyright 1999 Academic Press.

  11. PENGGUNAAN EKSTRAK BIOTA LAUTAGLAOPHENIA SP. UNTUK MENGENDALIKANFUSARIUM OXYSPORUM F.SP.VANILLAE PENYEBAB BUSUK BATANG VANILI

    Directory of Open Access Journals (Sweden)

    I KETUT SUADA

    2012-11-01

    Full Text Available The Use of Aglaophenia sp. Marine Biota Extract to Control Fusarium oxysporum f. sp. vanillae, a Pathogen of Vanilla Stem Rot The use of marine biota for organic fungicide material has enormous potential to be developed. The organic fungicide was directed to substitute synthetic fungicide for vanilla cultivation. Application of synthetic fungicide degraded environmental quality, therefore its applications does not support sustainable agriculture. The objective of this research was to know the potence of Aglaopheniain suppressing the growth of F. oxysporum f. sp.vanillae, the pathogen of vanilla stem rot. The research was done in green house in order to find the best substance for formulation of the extract. The substances screened were water, tween-80, and detergents with two different concentrations. Subsequently, two best formulas were chosen to apply in endemic area in Tabanan. The green house and field trial research used Randomized Completely Design with three and five replications respectively. Results showed that tween was the best substance used for formulation and concentration of 0.2% was better than other concentrations. The extract diluted in tween-80 on the concentration of 0.2% showed the lowest rot on stem, longest shoot, and the most leaves on vanilla shoot. Because of its significant performance, the 0.1% extract ofAglaopheniain 0.1% tween-80 was proposed to be the best formula to suppress the vanilla stem rot disease.

  12. DAYA HAMBAT INFUSA RIMPANG KUNYIT (Curcuma longa Linn TERHADAP PERTUMBUHAN Escherichia coli dan Vibrio sp. pada IKAN KERAPU LUMPUR (Epinephelus tauvina di PASAR KEDONGANAN KABUPATEN BADUNG, BALI

    Directory of Open Access Journals (Sweden)

    Ni Putu Sinta Puspa Dewi

    2017-09-01

    inhibited turmeric infusa rhizome is determined by counting the population of bacteria test after treatment by the method of dilution sampling (Plating Method. The results showed that turmeric rhizome infusion was able significantly (P<0,05 inhibitionto the growth of E. coli and Vibrio sp. both in vitro and in vivo. The control (0% in vitro population E. coli and Vibrio sp. each of 5,23x102 CFU/g and 4,98x102 CFU/g higher than with the treatment of concentration 5%, 10%, 15% and 20%. Population E. coli and Vibrio sp. in testing by in vivo (concentration 0% each is obtained 4,17x102 CFU/g dan 4,20x102 CFU/g in statistic is different (P<0,05 with the concentration 10%, 15% and 20%. Keywords: Epinephelus tauvina, Curcuma longa Linn, E. coli, Vibrio sp.

  13. Single Cystosorus Isolate Production and Restriction Fragment Length Polymorphism Characterization of the Obligate Biotroph Spongospora subterranea f. sp. subterranea.

    Science.gov (United States)

    Qu, Xinshun; Christ, Barbara J

    2006-10-01

    ABSTRACT Spongospora subterranea f. sp. subterranea causes powdery scab in potatoes and is distributed worldwide. Genetic studies of this pathogen have been hampered due, in part, to its obligate parasitism and the lack of molecular markers for this pathogen. In this investigation, a single cystosorus inoculation technique was developed to produce large amounts of S. subterranea f. sp. subterranea plasmodia or zoosporangia in eastern black nightshade (Solanum ptycanthum) roots from which DNA was extracted. Cryopreservation of zoosporangia was used for long-term storage of the isolates. S. subterranea f. sp. subterranea-specific restriction fragment length polymorphism (RFLP) markers were developed from randomly amplified polymorphic DNA (RAPD) fragments. Cystosori of S. subterranea f. sp. subterranea were used for RAPD assays and putative pathogen-specific RAPD fragments were cloned and sequenced. The fragments were screened for specificity by Southern hybridization and subsequent DNA sequence BLAST search. Four polymorphic S. subterranea f. sp. subterranea-specific probes containing repetitive elements, and one containing single copy DNA were identified. These RFLP probes were then used to analyze 24 single cystosorus isolates derived from eight geographic locations in the United States and Canada. Genetic variation was recorded among, but not within, geographic locations. Cluster analysis separated the isolates into two major groups: group I included isolates originating from western North America, with the exception of those from Colorado, and group II included isolates originating from eastern North America and from Colorado. The techniques developed in this study, i.e., production of single cystosorus isolates of S. subterranea f. sp. subterranea and development of RFLP markers for this pathogen, provide methods to further study the genetic structure of S. subterranea f. sp. subterranea.

  14. Polymorphisms of SP110 Are Associated with both Pulmonary and Extra-Pulmonary Tuberculosis among the Vietnamese

    Science.gov (United States)

    Fox, Gregory J.; Sy, Dinh Ngoc; Nhung, Nguyen Viet; Yu, Bing; Ellis, Magda K.; Van Hung, Nguyen; Cuong, Nguyen Kim; Thi Lien, Luu; Marks, Guy B.; Saunders, Bernadette M.; Britton, Warwick J.

    2014-01-01

    Background Tuberculosis (TB) is an infectious disease that remains a major cause of morbidity and mortality worldwide, yet the reasons why only 10% of people infected with Mycobacterium tuberculosis go on to develop clinical disease are poorly understood. Genetically determined variation in the host immune response is one factor influencing the response to M. tuberculosis. SP110 is an interferon-responsive nuclear body protein with critical roles in cell cycling, apoptosis and immunity to infection. However association studies of the gene with clinical TB in different populations have produced conflicting results. Methods To examine the importance of the SP110 gene in immunity to TB in the Vietnamese we conducted a case-control genetic association study of 24 SP110 variants, in 663 patients with microbiologically proven TB and 566 unaffected control subjects from three tertiary hospitals in northern Vietnam. Results Five SNPs within SP110 were associated with all forms of TB, including four SNPs at the C terminus (rs10208770, rs10498244, rs16826860, rs11678451) under a dominant model and one SNP under a recessive model, rs7601176. Two of these SNPs were associated with pulmonary TB (rs10208770 and rs16826860) and one with extra-pulmonary TB (rs10498244). Conclusion SP110 variants were associated with increased susceptibility to both pulmonary and extra-pulmonary TB in the Vietnamese. Genetic variants in SP110 may influence macrophage signaling responses and apoptosis during M. tuberculosis infection, however further research is required to establish the mechanism by which SP110 influences immunity to tuberculosis infection. PMID:25006821

  15. Kinetics of petroleum oil biodegradation by a consortium of three protozoan isolates (Aspidisca sp., Trachelophyllum sp. and Peranema sp.

    Directory of Open Access Journals (Sweden)

    L. Kachieng’a

    2017-09-01

    Full Text Available Petroleum oil is a complex mixture of substances, the majority of which are hydrocarbons; the latter represent an extremely important and heterogeneous group of compounds that find their way into water resources by anthropogenic or natural ways. The majority of toxic hydrocarbon components of petroleum are biodegradable, where bioremediation using microbial species has become an integral process for the restoration of oil-polluted areas. In this study, three bioremediation processes, namely natural attenuation, nutrient supplementation by adding glucose and biostimulation by adding Tween® 80, were carried out in various petroleum hydrocarbon concentrations in polluted water media using a consortium of three protozoan isolates (Aspidisca sp., Trachelophyllum sp. and Peranema sp.. A first-order kinetics model was fitted to the biodegradation data to evaluate the biodegradation rate and to determine the corresponding half-life time. First-order kinetics satisfactorily described the biodegradation of the petroleum-based contaminants under abiotic conditions. The results showed an increase in the percentage removal of petroleum oil at the lower petroleum concentrations and a gradual percentage decrease in removing petroleum oil residues occurred when there was an increase in the initial concentrations of the petroleum oil: 39%, 27%, 22%, 12%, 10% for various petroleum oil concentrations of 50, 100, 150, 200, 250 mg/L, respectively. A similar trend was also observed in the glucose-supplemented culture media where the reduction was 45% and 78% for petroleum concentrations of 250 mg/L and 50 mg/L, respectively. Biodegradation of between 33 and 90% was achieved at a Tween® 80 concentration of between 50 mg/L and 250 mg/L. The degradation rate constants for the natural attenuation process ranged between ≥0 to ≤0.50, ≥0 to ≤0.35, ≥0 to ≤0.25, ≥0 to ≤ 0.14 and ≥ 0 to ≤0.11 for petroleum oil concentrations varying from 50, 100, 150

  16. Antifungal Activity of Lactobacillus sp. Bacteria in the Presence of Xylitol and Galactosyl-Xylitol

    Directory of Open Access Journals (Sweden)

    Lidia Lipińska

    2016-01-01

    Full Text Available Lactic acid fermentation is a natural method of antimicrobial food protection. Antagonistic activity of Lactobacillus sp. bacteria, taking part in this process, is directed mainly against the same or other microorganisms. In this work we determine the impact of the presence of xylitol and galactosyl-xylitol on the antagonistic activity of 60 Lactobacillus sp. strains against indicator molds (Alternaria alternata, Alternaria brassicicola, Aspergillus niger, Fusarium latenicum, Geotrichum candidum, and Mucor hiemalis and yeasts (Candida vini. We used double-layer method to select antifungal strains of Lactobacillus bacteria and poisoned medium method to confirm their fungistatic properties. Additionally, we examined the inhibition of Alternaria brassicicola by Lactobacillus paracasei ŁOCK 0921 cultivated with xylitol or galactosyl-xylitol directly on wild cherries. The presence of xylitol and its galactosyl derivative led to increase of spectrum of antifungal activity in most of the studied plant-associated lactobacilli strains. However, no single strain exhibited activity against all the indicator microorganisms. The antifungal activity of Lactobacillus bacteria against molds varied considerably and depended on both the indicator strain and the composition of the medium. The presence of xylitol and galactosyl-xylitol in the growth medium is correlated with the antifungal activity of the studied Lactobacillus sp. bacteria against selected indicator molds.

  17. Antifungal Activity of Lactobacillus sp. Bacteria in the Presence of Xylitol and Galactosyl-Xylitol.

    Science.gov (United States)

    Lipińska, Lidia; Klewicki, Robert; Klewicka, Elżbieta; Kołodziejczyk, Krzysztof; Sójka, Michał; Nowak, Adriana

    2016-01-01

    Lactic acid fermentation is a natural method of antimicrobial food protection. Antagonistic activity of Lactobacillus sp. bacteria, taking part in this process, is directed mainly against the same or other microorganisms. In this work we determine the impact of the presence of xylitol and galactosyl-xylitol on the antagonistic activity of 60 Lactobacillus sp. strains against indicator molds (Alternaria alternata, Alternaria brassicicola, Aspergillus niger, Fusarium latenicum, Geotrichum candidum, and Mucor hiemalis) and yeasts (Candida vini). We used double-layer method to select antifungal strains of Lactobacillus bacteria and poisoned medium method to confirm their fungistatic properties. Additionally, we examined the inhibition of Alternaria brassicicola by Lactobacillus paracasei ŁOCK 0921 cultivated with xylitol or galactosyl-xylitol directly on wild cherries. The presence of xylitol and its galactosyl derivative led to increase of spectrum of antifungal activity in most of the studied plant-associated lactobacilli strains. However, no single strain exhibited activity against all the indicator microorganisms. The antifungal activity of Lactobacillus bacteria against molds varied considerably and depended on both the indicator strain and the composition of the medium. The presence of xylitol and galactosyl-xylitol in the growth medium is correlated with the antifungal activity of the studied Lactobacillus sp. bacteria against selected indicator molds.

  18. Antifungal Activity of Lactobacillus sp. Bacteria in the Presence of Xylitol and Galactosyl-Xylitol

    Science.gov (United States)

    Lipińska, Lidia; Klewicki, Robert; Klewicka, Elżbieta; Kołodziejczyk, Krzysztof; Sójka, Michał; Nowak, Adriana

    2016-01-01

    Lactic acid fermentation is a natural method of antimicrobial food protection. Antagonistic activity of Lactobacillus sp. bacteria, taking part in this process, is directed mainly against the same or other microorganisms. In this work we determine the impact of the presence of xylitol and galactosyl-xylitol on the antagonistic activity of 60 Lactobacillus sp. strains against indicator molds (Alternaria alternata, Alternaria brassicicola, Aspergillus niger, Fusarium latenicum, Geotrichum candidum, and Mucor hiemalis) and yeasts (Candida vini). We used double-layer method to select antifungal strains of Lactobacillus bacteria and poisoned medium method to confirm their fungistatic properties. Additionally, we examined the inhibition of Alternaria brassicicola by Lactobacillus paracasei ŁOCK 0921 cultivated with xylitol or galactosyl-xylitol directly on wild cherries. The presence of xylitol and its galactosyl derivative led to increase of spectrum of antifungal activity in most of the studied plant-associated lactobacilli strains. However, no single strain exhibited activity against all the indicator microorganisms. The antifungal activity of Lactobacillus bacteria against molds varied considerably and depended on both the indicator strain and the composition of the medium. The presence of xylitol and galactosyl-xylitol in the growth medium is correlated with the antifungal activity of the studied Lactobacillus sp. bacteria against selected indicator molds. PMID:27294124

  19. Cr(VI) reduction from contaminated soils by Aspergillus sp. N2 and Penicillium sp. N3 isolated from chromium deposits.

    Science.gov (United States)

    Fukuda, Tsubasa; Ishino, Yasuhiro; Ogawa, Akane; Tsutsumi, Kadzuyo; Morita, Hiroshi

    2008-10-01

    Aspergillus sp. N2 and Penicillium sp. N3 are chromate-resistant filamentous fungi that were isolated from Cr(VI) contaminated soil based on their ability to decrease hexavalent chromium levels in the growth medium. After 120 h of growth in a medium containing 50 ppm Cr(VI) at near neutral pH, Aspergillus sp. N2 reduced the Cr(VI) concentration by about 75%. Penicillium sp. N3 was able to reduce the Cr(VI) concentration by only 35%. However, Penicillium sp. N3 reduced the Cr(VI) concentration in the medium by 93% under acidic conditions. Interestingly, the presence of Cu(II) enhanced the Cr(VI) reducing ability of Aspergillus sp. N2 and Penicillium sp. N3 at near neutral pH. Aspergillus sp. N2 and Penicillium sp. N3 reduced the Cr(VI) concentration in the growth medium to a virtually undetectable level within 120 h. For both Aspergillus sp. N2 and Penicillium sp. N3, mycelial seed cultures were more efficient at Cr(VI) reduction than conidium seed cultures. The mechanisms of Cr(VI) reduction in Aspergillus sp. N2 and Penicillium sp. N3 were enzymatic reduction and sorption to mycelia. Enzymatic activity contributed significantly to Cr(VI) reduction. Aspergillus sp. N2 and Penicillium sp. N3 reduced the levels of Cr(VI) in polluted soil samples, suggesting that these strains might be useful for cleaning up chromium-contaminated sites.

  20. DAN IDENTIFIKASI PATOGEN POTENSIAL YANG MENGINFEKSI IKAN RAINBOW (Melanotaenia sp.

    Directory of Open Access Journals (Sweden)

    Lili Sholichah

    2014-03-01

    Full Text Available Pemeliharaan ikan rainbow (Melanotaenia sp. di Balai Penelitian dan Pengembangan Budidaya Ikan Hias selalu terjadi kematian secara bertahap mulai calon induk hingga proses pemijahan. Hal ini terjadi berulang kali sehingga ketersediaan induk Melanotaenia sp. sangat terancam. Ikan ini berasal dari Papua yang diperoleh mengandalkan penangkapan di alam. Tujuan dari penelitian ini adalah untuk menginventarisir dan mengidentifikasi berbagai patogen (parasit, jamur, bakteri potensial yang menginfeksi ikan rainbow yang dipelihara di dalam akuarium berukuran 50 cm x 50 cm x 50 cm dengan sistem aliran air stagnan. Tiga jenis rainbow yang dipelihara yaitu: rainbow Sungai Salawati, asal Sungai Sawiat, dan asal Danau Kurumoi. Setiap ikan masing-masing berjumlah 100 ekor dipelihara di akuarium dengan penambahan batu karang dan tanpa penambahan karang (kontrol ke dalam akuarium. Ikan diberi pakan sekenyangnya berupa jentik nyamuk dan cacing rambut beku setiap pagi dan sore hari. Sampling dilakukan secara random sebulan sekali dan secara unrandom setiap ada kejadian ikan sakit. Gejala klinis ikan yang sakit sebagai berikut: ikan berenang di permukaan dan menggosok-gosokkan badan di dinding akuarium, nafsu makan berkurang, gerakan berputar-putar, warna memudar menjadi putih, penekanan warna hitam pada sirip punggung dan perut meningkat, pendarahan pada perut, lendir berlebihan dan sangat berbau, serta sisik berdiri/terbuka. Diagnosa dan deteksi penyakit awal berupa pengamatan parasit baik ektoparasit maupun endoparasit, pengamatan dan isolasi jamur pada media selektif jamur, dan isolasi bakteri dilakukan untuk mengetahui jenis-jenis patogen yang menginfeksi ketiga jenis ikan rainbow. Selanjutnya dilakukan uji histologi dan analisa DNA beberapa patogen. Hasil pengamatan diperoleh patogen berupa parasit (Ichthyophthirius sp., Dactylogyrus sp., Gyrodactylus sp., dan Trichodina sp. dan bakteri (Aeromonas hydrophila, Acinetobacter sp

  1. Genetic polymorphism of horse serum protein 3 (SP3).

    Science.gov (United States)

    Juneja, R K; Sandberg, K; Kuryl, J; Gahne, B

    1989-01-01

    Two-dimensional agarose gel (pH 8.6)-horizontal polyacrylamide gel (pH 9.0) electrophoresis of horse serum samples, followed by general protein staining, revealed genetic polymorphism of an unidentified protein tentatively designated serum protein 3 (SP3). The SP3 fractions appeared distinctly when a 14% concentration of acrylamide was used in the separation gels. The 2-D mobilities of SP3 fractions were quite similar to that of albumin. Family data were consistent with the hypothesis that the observed SP3 phenotypes were controlled by four co-dominant, autosomal alleles (D, F, I, S). Evidence was provided that the F allele can be further divided into two alleles (F1 and F2); the mobilities of F1 and F2 variants were very similar. Each of the SP3 alleles gave rise to one fraction and each of the heterozygous types showed two fractions. More than 600 horses representing five different breeds (Swedish Trotter, North-Swedish Trotter, Thoroughbred, Arab and Polish Tarpan) were typed for SP3, and allele frequency estimates were calculated. SP3 was highly polymorphic in all breeds studied.

  2. Streptopyridines, volatile pyridine alkaloids produced by Streptomyces sp. FORM5

    Directory of Open Access Journals (Sweden)

    Ulrike Groenhagen

    2014-06-01

    Full Text Available Streptomyces sp. FORM5 is a bacterium that is known to produce the antibiotic streptazolin and related compounds. We investigated the strain for the production of volatiles using the CLSA (closed-loop stripping analysis method. Liquid and agar plate cultures revealed the formation of new 2-alkylpyridines (streptopyridines, structurally closely related to the already known 2-pentadienylpiperidines. The structures of the streptopyridines A to E were confirmed by total synthesis. The analysis of the liquid phase by solvent extraction or extraction with an Oasis adsorbent showed that streptazolin and 2-pentadienylpiperidine are the major compounds, while the streptopyridines are only minor components. In the gas phase, only the streptopyridines could be detected. Therefore, an orthogonal set of analysis is needed to assess the metabolic profile of bacteria, because volatile compounds are obviously overlooked by traditional analytical methods. The streptopyridines are strain specific volatiles that are accompanied by a broad range of headspace constituents that occur in many actinomycetes. Volatiles might be of ecological importance for the producing organism, and, as biosynthetic intermediates or shunt products, they can be useful as indicators of antibiotic production in a bacterium.

  3. Radiation characteristics and effective optical properties of dumbbell-shaped cyanobacterium Synechocystis sp

    International Nuclear Information System (INIS)

    Heng, Ri-Liang; Pilon, Laurent

    2016-01-01

    This study presents experimental measurements of the radiation characteristics of unicellular freshwater cyanobacterium Synechocystis sp. during their exponential growth in F medium. Their scattering phase function at 633 nm average spectral absorption and scattering cross-sections between 400 and 750 nm were measured. In addition, an inverse method was used for retrieving the spectral effective complex index of refraction of overlapping or touching bispheres and quadspheres from their absorption and scattering cross-sections. The inverse method combines a genetic algorithm and a forward model based on Lorenz–Mie theory, treating bispheres and quadspheres as projected area and volume-equivalent coated spheres. The inverse method was successfully validated with numerically predicted average absorption and scattering cross-sections of suspensions consisting of bispheres and quadspheres, with realistic size distributions, using the T-matrix method. It was able to retrieve the monomers' complex index of refraction with size parameter up to 11, relative refraction index less than 1.3, and absorption index less than 0.1. Then, the inverse method was applied to retrieve the effective spectral complex index of refraction of Synechocystis sp. approximated as randomly oriented aggregates consisting of two overlapping homogeneous spheres. Both the measured absorption cross-section and the retrieved absorption index featured peaks at 435 and 676 nm corresponding to chlorophyll a, a peak at 625 nm corresponding to phycocyanin, and a shoulder around 485 nm corresponding to carotenoids. These results can be used to optimize and control light transfer in photobioreactors. The inverse method and the equivalent coated sphere model could be applied to other optically soft particles of similar morphologies. - Highlights: • Radiation characteristics of Synechocystis sp. were measured during exponential growth. • This unicellular freshwater cyanobacterium features an interesting

  4. Isolation and Characterization of an Atypical Metschnikowia sp. Strain from the Skin Scraping of a Dermatitis Patient.

    Directory of Open Access Journals (Sweden)

    Chee Sian Kuan

    Full Text Available A yeast-like organism was isolated from the skin scraping sample of a stasis dermatitis patient in the Mycology Unit Department of Medical Microbiology, University Malaya Medical Centre (UMMC, Kuala Lumpur, Malaysia. The isolate produced no pigment and was not identifiable using chromogenic agar and API 20C AUX. The fungus was identified as Metschnikowia sp. strain UM 1034, which is close to that of Metschnikowia drosophilae based on ITS- and D1/D2 domain-based phylogenetic analysis. However, the physiology of the strain was not associated to M. drosophilae. This pathogen exhibited low sensitivity to all tested azoles, echinocandins, 5-flucytosine and amphotericin B. This study provided insight into Metschnikowia sp. strain UM 1034 phenotype profiles using a Biolog phenotypic microarray (PM. The isolate utilized 373 nutrients of 760 nutrient sources and could adapt to a broad range of osmotic and pH environments. To our knowledge, this is the first report of the isolation of Metschnikowia non-pulcherrima sp. from skin scraping, revealing this rare yeast species as a potential human pathogen that may be misidentified as Candida sp. using conventional methods. Metschnikowia sp. strain UM 1034 can survive in flexible and diverse environments with a generalist lifestyle.

  5. Biotransformation of two furanocoumarins by the fungi species Aspergillus sp. PTCC 5266 and Aspergillus niger PTCC 5010.

    Science.gov (United States)

    Ghasemi, Saba; Habibi, Zohreh; Mohajeri, Maryam; Yousefi, Maryam

    2018-02-22

    The microbial transformations of peucedanin and oreoselon by the fungi Aspergillus niger and Aspergillus sp. were investigated for the first time. Incubation of peucedanin with A. niger yielded a new hydroxylated metabolite with high yield (56%), which was characterized as 2-(1-hydroxypropan-2-yl)-3-methoxy-7H-furo[3,2-g]chromen-7-one. Oreoselon was converted to a new reduced metabolite methyl 3-(2,3-dihydro-6-hydroxy-2-isopropyl-3-oxobenzofuran-5-yl)propanoate in biotransformation by Aspergillus sp. The structures of the metabolites were determined by spectroscopic methods including IR, EI-MS, 1 H NMR, 13 C NMR, and elemental analysis.

  6. Agrobacterium rhizogenes mediated transformation of Rhodiola sp. – an approach to enhance the level of bioactive compounds

    DEFF Research Database (Denmark)

    Møller Hansen, Martin; Lauridsen, Uffe Bjerre; Hegelund, Josefine Nymark

    Agrobacterium rhizogenes mediated transformation of Rhodiola sp. – an approach to enhance the level of bioactive compounds. Martin Møller Hansen1, Uffe Bjerre Lauridsen2, Josefine Nymark Hegelund3, Renate Müller4, Jihong Liu Clarke5, Henrik Lütken6 University of Copenhagen, Faculty of Science...... to wild type roots. The purpose of this study is to obtain HRs containing rol-genes from Rhodiola sp. for future sustainable production in bioreactors. Materials and Methods Whole stems of R. rosea and two accessions of R. pachyclados were sterilized with ethanol and NaOCl. The stems were then cut...

  7. Halomonas sp. BS4, A biosurfactant producing halophilic bacterium isolated from solar salt works in India and their biomedical importance

    OpenAIRE

    Donio, Mariathason Birdilla Selva; Ronica, Fernando Arul; Viji, Vijayaragavan Thanga; Velmurugan, Subramanian; Jenifer, John Selesteen Charles Adlin; Michaelbabu, Mariavincent; Dhar, Prasenjit; Citarasu, Thavasimuthu

    2013-01-01

    Halophilic bacteria were isolated from Thamaraikulam solar salt works in India. After routine biosurfactant screening by various methods, the biosurfactant producing bacteria, Halomonas sp BS4 was confirmed by 16?S rRNA sequencing. The growth optimization of Halomonas sp BS4 revealed their optimum growth at 8% NaCl and 6-8?pH in the growth medium. Further the partially purified biosurfactants were characterized by TLC, FTIR and GC-MS analysis. GC-MS results revealed that, the partial purified...

  8. Exopisiod B and farylhydrazone C, two new alkaloids from the Antarctic-derived fungus Penicillium sp. HDN14-431.

    Science.gov (United States)

    Zhang, Ting; Zhu, Mei-Lin; Sun, Guang-Yu; Li, Na; Gu, Qian-Qun; Li, De-Hai; Che, Qian; Zhu, Tian-Jiao

    2016-10-01

    Two new compounds, exopisiod B (1) and farylhydrazone C (2), together with two known compounds (3-4), were isolated from the Antarctic-derived fungus Penicillium sp. HDN14-431. Their structures including absolute configurations were elucidated by spectroscopic methods and TDDFT ECD calculations. The cytotoxicity and antimicrobial activities of all compounds were tested.

  9. Reparation and Immunomodulating Properties of Bacillus sp. Metabolites from Permafrost.

    Science.gov (United States)

    Kalenova, L F; Melnikov, V P; Besedin, I M; Bazhin, A S; Gabdulin, M A; Kolyvanova, S S

    2017-09-01

    An ointment containing metabolites of Bacillus sp. microorganisms isolated from permafrost samples was applied onto the skin wound of BALB/c mice. Metabolites isolated during culturing of Bacillus sp. at 37°C produced a potent therapeutic effect and promoted wound epithelialization by 30% in comparison with the control (ointment base) and by 20% in comparison with Solcoseryl. Treatment with Bacillus sp. metabolites stimulated predominantly humoral immunity, reduced the time of wound contraction and the volume of scar tissue, and promoted complete hair recovery. These metabolites can be considered as modulators of the wound process with predominance of regeneration mechanisms.

  10. Efektivitas Ovitrap Bambu terhadap Jumlah Jentik Aedes sp yang Terperangkap

    Directory of Open Access Journals (Sweden)

    Wiwit Aditama

    2015-05-01

    places of 10 subdistricts. Data of trapped Aedes sp larvae numbers was collected four times repeatedly within one week time-lapse. Analysis was conducted using the mean number of larvae in ovitraps and ovitrap index. The number of Aedes sp larvae trapped was 1,265. The most effective ovitrap is piece of bamboo, mean = 123, p value = 0.006, HI = 10.01% (16.66 26.67%, CI = 36.8% (336.06 - 39.74%, BI = 29.97% (73.33 - 103.3%. Health authorities should promote bamboo ovitrap, especially to public as an effort to control Aedes sp.

  11. Späte Mutterschaft – (keine biografische Entscheidung

    Directory of Open Access Journals (Sweden)

    Martina Beham

    2004-11-01

    Full Text Available Der Übergang in die Mutterschaft findet zunehmend später statt. Über die Gründe liegen zahlreiche Vermutungen und einige theoretische Diskussionen, aber kaum empirische Befunde vor. Die Autorinnen möchten mit ihrer Studie einen Beitrag zum Abbau dieses Forschungsdefizits leisten. In ihrer empirisch-biografischen Untersuchung gehen Ingrid Herlyn und Dorothea Krüger der Frage nach, ob späte Erstmutterschaft ein neues biografisches Muster der Familiengründung darstellt oder ob Mutterschaft nach wie vor eine selbstverständlich anerkannte Norm ist, deren Realisierung im Lebenslauf lediglich zeitlich später stattfindet.

  12. Thermodynamically Controlled High-Pressure High-Temperature Synthesis of Crystalline Fluorinated sp 3 -Carbon Networks

    Energy Technology Data Exchange (ETDEWEB)

    Klier, Kamil; Landskron, Kai

    2015-11-19

    We report the feasibility of the thermodynamically controlled synthesis of crystalline sp3-carbon networks. We show that there is a critical pressure below which decomposition of the carbon network is favored and above which the carbon network is stable. Based on advanced, highly accurate quantum mechanical calculations using the all-electron full-potential linearized augmented plane-wave method (FP-LAPW) and the Birch–Murnaghan equation of state, this critical pressure is 26.5 GPa (viz. table of contents graphic). Such pressures are experimentally readily accessible and afford thermodynamic control for suppression of decomposition reactions. The present results further suggest that a general pattern of pressure-directed control exists for many isolobal conversions of sp2 to sp3 allotropes, relating not only to fluorocarbon chemistry but also extending to inorganic and solid-state materials science.

  13. THE EFFECT OF FORMULATION HUMIC SUBSTANCE AND Trichoderma sp TO INCREASE PRODUCTION AND GROWTH OF CORN (Zea Mays,L

    Directory of Open Access Journals (Sweden)

    Ruly Eko Kusuma Kurniawan

    2017-06-01

    Full Text Available Research to determine of formulation humic subtance and Trichoderma sp to increase the production and growth of corn (zea mays,L. This research was conducted by extracting humic substance with fractionation organic matter method from cattle manure organic material. Trichoderma sp grow on corn medium and harvested after reaching a density of 1015 cfu. Created this compound formulation with mixing humic substance and Trichoderma sp. Indicator plant with F1 sweet corn Jago varieties. Aplication used humic substance in range 8%, 16%, and 32% on the recommended use NPK fertilizer for corn, as well as control without humic substance. The result showed application use 8% humic substance most good for plant growth and harvest. Additionally, nutrient uptake NPK fertilizer efficiency is increased and more effective than control and use of the formulation 16% and 32% humic substance.

  14. Filtering of canted magnetic phase of SpFeMn(C2O4)3 in membrane nanopores

    International Nuclear Information System (INIS)

    Dmitriev, A.I.; Morgunov, R.B.

    2015-01-01

    Ordered arrays of nanowires of pure SpFeMn(C 2 O 4 ) 3 collinear antiferromagnet have been synthesized in pores of anodized aluminum oxide (AAO) 20 nm and 200 nm in diameter. It was found that the growth of spin-canted phase presented in the bulk samples is suppressed in nanowires. The decrease in the nanowires diameter results in magneto-crystalline anisotropy increase. - Highlights: • The method for separation of two magnetic phases of SpFeMn(C 2 O 4 ) 3 was developed. • Nanowires of collinear antiferromagnet SpFeMn(C 2 O 4 ) 3 was synthesized in pores of AAO. • Suppression of canted phase presented in the bulk samples was found in nanowires. • Magnetic anisotropy indicates monocrystallinity of nanowires grown in AAO pores. • The decrease of nanowires diameter results in magnetocrystalline anisotropy increase

  15. Borrelia sp. phylogenetically different from Lyme disease- and relapsing fever-related Borrelia spp. in Amblyomma varanense from Python reticulatus.

    Science.gov (United States)

    Trinachartvanit, Wachareeporn; Hirunkanokpun, Supanee; Sudsangiem, Ronnayuth; Lijuan, Wanwisa; Boonkusol, Duangjai; Baimai, Visut; Ahantarig, Arunee

    2016-06-24

    Species of the genus Borrelia are causative agents of Lyme disease and relapsing fever. Lyme disease is the most commonly reported vector-borne disease in the northern hemisphere. However, in some parts of the world Lyme borreliosis and relapsing fever may be caused by novel Borrelia genotypes. Herein, we report the presence of a Borrelia sp. in an Amblyomma varanense collected from Python reticulatus. Ticks were collected from snakes, identified to species level and examined by PCR for the presence of Borrelia spp. flaB and 16S rRNA genes. Phylogenetic trees were constructed using the neighbour-joining method. Three A. varanense ticks collected from P. reticulatus were positive for a unique Borrelia sp., which was phylogenetically divergent from both Lyme disease- and relapsing fever-associated Borrelia spp. The results of this study suggest for the first time that there is a Borrelia sp. in A. varanense tick in the snake P. reticulatus that might be novel.

  16. Beneficial Effects of Rhodotorula sp. C11 on Growth and Disease Resistance of Juvenile Japanese Spiky Sea Cucumber Apostichopus japonicus.

    Science.gov (United States)

    Yang, ZhiPing; Sun, JianMing; Xu, Zhe

    2015-06-01

    The purpose of this study was to evaluate the effects of dietary administration of the live yeast, Rhodotorula sp. C11, on growth and disease resistance against Vibrio splendidus infection in juvenile Japanese spiky sea cucumber Apostichopus japonicus. Sea cucumbers were fed diets containing Rhodotorula sp. C11 at 0 (control), 10⁴, 10⁵, and 10⁶ CFU/g of feed for 45 d. There were three replicate tanks per dietary treatment. The specific growth rates were higher in all sea cucumbers treated with Rhodotorula sp. C11 than in the controls. Following a challenge with V. splendidus NB13, the cumulative prevalence and mortality of sea cucumbers fed diets supplemented with Rhodotorula sp. C11 were lower than in animals fed the basal diet. In sea cucumbers fed diets supplemented with Rhodotorula sp. C11 for 42 d, the only viable yeast found in the intestine was Rhodotorula sp. C11, which had counts of 1.58-1.98 × 10⁴CFU/g. No yeast was isolated from the intestine of animals fed the basal diet. For the colonization study, 20 sea cucumbers from each dietary treatment were removed to separate tanks and fed the control diet from day 16 to day 46. The viable yeast (Rhodotorula sp. C11) counts in the intestine decreased to 60-80 CFU/g by day 37. Moreover, as demonstrated by denaturing gradient gel electrophoresis, Rhodotorula sp. C11 colonization of the intestine could be detected until day 46. The differences in culture and PCR-denaturing gradient gel electrophoresis may be due to differences in the sensitivity of both methods. The present result showed that Rhodotorula sp. C11 was able to successfully colonize the intestine of juvenile Japanese spiky sea cucumbers by dietary supplementation, which improved its growth and disease resistance.

  17. The Effect of maceration period on contents and color brightness of phycoerythrin from Gracilaria sp.

    Science.gov (United States)

    Lidiana, H.; Sulmartiwi, L.; Andriyono, S.

    2018-04-01

    Natural pigment from seaweed, is currently required by humans as one of active compounds that are usefull in the field of health, cosmetics and food. One of the pigments which potentially can be developed is red phycobilin pigment. The pigment can be obtained from Gracilaria sp. throught maceration method. This research aims to find out wheter there is influence from the long maceration time toward to the content and the phycoerytrin color brightness of Gracilaria sp. The reseach method used experimental experiment with complete randomized design (CRD) which was consisted of nine treatments of long time maceration period three replications. Data analysis was used the Analysis of Variance (ANOVA) and continued to Duncan’s Multiple Distance Test to. The result showed that treatments with the time of maceration for 30 hours was the best treatment on phycoerytrin content about 0.98 mg/g with the brightness value was 19.73 L*, reddish value was 7.23 a*, and yellowish value was 2.87 b* of Gracilaria sp.

  18. MORSEC-SP, Step Function Angular Distribution for Cross-Sections Calculation by Program MORSE

    International Nuclear Information System (INIS)

    1980-01-01

    1 - Description of problem or function: MORSEC-SP allows one to utilize a step distribution to describe the angular dependence of the multi- group function in the MORSEC cross section module of the MORSE Monte Carlo code. The step distribution is always non-negative and may be used in the random walk and for making point detector estimators. 2 - Method of solution: MORSEC-SP utilizes a table look up procedure to provide the probability of scattering when making point detector estimates for a given incident energy group and scattering angle. In the random walk, the step distributions are converted to cumulative distributions and an angle of scatter is selected from the cumulative distributions. Step distributions are obtained from calculation using the converted moments from the given Legendre coefficients of the scattering distributions. 3 - Restrictions on the complexity of the problem: Additional coding to the MORSEC module is variable dimensional and fully incorporated into blank common

  19. A new aurone glycoside with antifungal activity from marine-derived fungus Penicillium sp. FJ-1.

    Science.gov (United States)

    Song, Yan-xia; Ma, Qiang; Li, Jie

    2015-03-01

    Endophytic fungi which reside in the tissue of mangrove plants seem to play an important role in the discovery of new biologically active substances. During the course of screening for the antimicrobial metabolites from the endophytic fugus Penicillium sp. FJ-1 of mangrove plant Avicennia marina, a new aurone glycoside (1) was isolated by repeated column chromatography on silica gel and recrystallization methods. The structure of 1 was elucidated as (Z)-7,4'-dimethoxy-6-hydroxy-aurone-4-O-β-glucopyranoside, on the basis of spectroscopic analysis. Compound 1 exhibited antifungal activity against Candida sp., with the potency comparable to amphotericin B and much better than fluconazole. Compound 1 can also inhibit extracellular phospholipase secretion in a concentration-dependent manner.

  20. Harvesting of freshwater microalgae biomass by Scenedesmus sp. as bioflocculant

    Science.gov (United States)

    Rinanti, A.; Purwadi, R.

    2018-01-01

    This study is particularly expected to provide information on the diversity of microalgae as the flocculant agent that gives the highest biomass yield. Bioflocculation was done by using one of the flocculating microalgae i.e. Scenedesmus obliquus to concentrate on non-flocculating microalgae Chlorella vulgaris. The freshwater microalgae S. obliquus tested it ability to harvest other non-flocculating microalgae, increased sedimentation rate in the flocculation process and increased biomass yield. The flocculation of biomass microalgae with chemical flocculant as comparison was done by adding alum (K2SO4·Al2 (SO4)3·24H2O). The addition of alum (K2SO4·Al2 (SO4)3·24H2O) as flocculant at pH 11 and S. obliquus sp. as bioflocculant caused significant alteration of nutrition of microalgae. Overall, the essential content produced by flocculation method with addition of alum or with bioflocculation (%, mg/100 mg dry weight) are lipid 31,64; 38,69, protein 30,79; 38.50%, and chlorophyll 0.6253; 0.8420). Harvesting with bioflocculation methods conducted at the end of the cultivation period increase the amount of biomass significantly and can accelerate the settling time of biomass. Harvesting microalgae cells by bioflocculation method becomes an economically competitive harvesting method compared to alum as a chemical flocculant because of the cheaper cost of flocculant, not toxic so it does not require further water treatment after harvesting due to the use of alum as chemical flocculants.