WorldWideScience

Sample records for segen durch gene

  1. Effizienzsteigerung durch intelligente Planung

    Science.gov (United States)

    Maier, Walther; Bader, Alexander

    Das Umfeld der produzierenden Unternehmen hat sich in den letzten Jahren sehr gewandelt. Dieser Wandel ist geprägt durch die Folgen der Globalisierung und deren Folgeerscheinungen. Produzierende Unternehmen versuchen auf diese geänderten Rahmenbedingungen durch Steigerung ihrer Produktivität und Flexibilität zu reagieren.

  2. Kreuz und quer durch Deutschland

    DEFF Research Database (Denmark)

    Hellensberg, Christina

    2015-01-01

    Undervisningsforløbet Kreuz und quer durch Deutschland beskriver læringsmålstyret undervisning i faget tysk i 5. og 6. klasse, hvor der arbejdes med kompetenceområderne mundtlig kommunikation samt kultur og samfund. Forløbet er forskningsinformeret og afprøvet i en 5. klasse.......Undervisningsforløbet Kreuz und quer durch Deutschland beskriver læringsmålstyret undervisning i faget tysk i 5. og 6. klasse, hvor der arbejdes med kompetenceområderne mundtlig kommunikation samt kultur og samfund. Forløbet er forskningsinformeret og afprøvet i en 5. klasse....

  3. Irritation durch Waschen und Desinfizieren

    OpenAIRE

    Weimer, Caroline Maria

    2006-01-01

    Ziel dieser Studie war die Irritation der Haut, hervorgerufen durch alkoholische Desinfektionsmittel und das Detergens Natriumlaurylsulfat (0,5% NLS) in einem repetitiven Testdesign zu untersuchen. Mittels nicht invasiver Untersuchungsmethoden quantifizierten wir die irritativen Effekte von Sterillium®, 2-Propanol 45% v/v, 1-Propanol 30% v/v, welches die alkoholische Grundlage von Sterillium® darstellt sowie von Wasser und NLS 0,5...

  4. Krank durch Sport: Anorexia athletica

    Directory of Open Access Journals (Sweden)

    Friedmann-Bette B

    2012-01-01

    Full Text Available Sportler aus Sportarten, in denen ein geringes Körpergewicht vorteilhaft für die Leistungsfähigkeit ist, gehen oft durch Reduktion der Kalorienzufuhr bei gleichzeitig hohem Energieverbrauch ein erhebliches Energiedefizit ein. Dabei kommt es häufig zu Essstörungen, für die in besonders gefährdeten Sportarten eine Prävalenz von bis zu 80 % berichtet wird und die sich zur Anorexia nervosa oder Bulimia nervosa entwickeln können. Als Folge des Energiedefizits können Konzentrationsänderungen von Stoffwechselhormonen, Störungen der Hypothalamus- Hypophysen-Gonaden-Achse und des Knochenstoffwechsels mit Abnahme der Knochendichte bis hin zur Osteoporose und einem erhöhten Risiko für Stressfrakturen beobachtet werden. Der Symptomkomplex aus Essstörung, sekundärer Amenorrhö und verminderter Knochendichte wird auch als „Female Athlete Triad“ bezeichnet, wobei in den vergangenen Jahren als viertes Symptom eine endotheliale Dysfunktion beschrieben wurde. Die effektivste Therapie ist das Herstellen eines dauerhaften Energiegleichgewichts, wofür eine tägliche Energieverfügbarkeit von mindestens 30 kcal/kg Magergewicht erforderlich ist. Eine hierdurch bedingte Gewichtszunahme kann den Sportlern nicht immer vermittelt werden, weshalb durch eine sorgfältig zusammengestellte Ernährung zumindest auf eine ausreichende Zufuhr von Kalzium, Vitamin D und Eiweiß geachtet werden muss. Oftmals ist die zusätzliche Verabreichung von Kalzium- und Vitamin-D-Präparaten angezeigt sowie eine Östrogensubstitution. Allerdings ist ein orales Kontrazeptivum als einzige Therapie – anders als früher angenommen – nicht ausreichend zur Behandlung oder Vermeidung einer Knochendichteminderung. Zur Prävention der Anorexia athletica sind die Aufklärung der Sportler, Trainer und Betreuer und ein Screening im Rahmen von Sporttauglichkeitsuntersuchungen, vor allem aber auch strukturelle Maßnahmen durch die Sportfachverbände erforderlich (wie zum Beispiel im

  5. Generierung synthetischer Hologramme durch Texture Mapping

    OpenAIRE

    Ritter, Alf; Böttger, Joachim; Deussen, Oliver

    1998-01-01

    Die synthetische Erzeugung von Hologrammen stellt hohe Anforderungen an Rechenleistung und Speicherkapazität. Traditionelle Methoden erlauben daher lediglich eine zeitaufwendige Hologrammgenerierung. Eine Beschleunigung wird durch den Einsatz von Methoden der Computergrafik erreicht, die die Ausnutzung von Grafikhardware gestatten. Dieser Beitrag beschreibt die Erzeugung synthetischer Hologramme mit Hilfe von Texturen. Dazu werden unterschiedliche Techniken der Überlagerung von Texturen disku...

  6. Differentielle Regulation der Chemokinsynthese in Alveolarepithelzellen durch Stickstoff- und Sauerstoffradikale

    OpenAIRE

    Bayer, Friederike Christiane

    2003-01-01

    Alveolarepithelzellen beteiligen sich durch Sekretion von Chemokinen und Expression von Adhäsionsmolekülen aktiv am Prozess der Leukozytenrekrutierung in den Alveolarraum. In der vorliegenden Arbeit wurde der Einfluss der Radikale Stickstoffmonoxid (NO), Superoxid (O2-) und ihrem Reaktionsprodukt Peroxynitrit (ONOO-) auf die spontane sowie auf die durch die proinflammatorischen Zytokine IL-1b und TNFa induzierte Synthese der Chemokine IL-8/CXCL8 und MCP-1/CCL2 in Alveolarepithelzellen untersu...

  7. Innovation durch Paradigmenwechsel - zur Bone Welding® Technologie

    Science.gov (United States)

    Mayer, Jörg; Plasonig, Gerhard

    Innovation entstand und entsteht in der Medizin häufig aus dem Bedürfnis des Klinikers heraus, bestehende chirurgische Techniken zu verbessern oder durch die Einführung neuer Methoden, chirurgische Zugänge zu ermöglichen, welche für den Patienten weniger traumatisch und für den Chirurgen technisch einfacher und damit sicherer sind. Historisch gesehen wurden Innovationen bis weit in die zweite Hälfte des 20. Jahrhunderts vor allem durch Kliniker angeregt und auch massgeblich mitentwickelt. Als illustrative Beispiele hierfür seien die heute immer noch führenden und auch kommerziell sehr erfolgreichen Entwicklungen von Osteosynthesetechniken und Implantaten durch die AO (Arbeitsgemeinschaft für Osteosynthesefragen) oder auch die Einführung endoskopischer Operationstechniken erwähnt.

  8. Herstellung polarisationsholografischer optischer Elemente durch Laserbelichtung in Azobenzen-Polymeren

    OpenAIRE

    Fratz, Markus

    2010-01-01

    Polarisationshologramme sind optische Elemente, deren Wirkung auf der Beeinflussung des Polarisationszustandes elektromagnetischer Wellen beruht. Eine Möglichkeit der Herstellung von Polarisationshologrammen besteht darin, mit Hilfe kurzwelligen, linear polarisierten Lichts (Wellenlänge kleiner 550 nm) Anisotropie in Azobenzen-Polymeren zu erzeugen. Die erzeugte Anisotropie ist nach der Belichtung makroskopisch als Doppelbrechung beobachtbar. Durch hochaufgelöste räumliche Variation dieser Do...

  9. Flexible Automatisierung durch Mensch-Roboter-Kollaboration: Mensch-Roboter-Kollaboration: Nutzen, Technik, Anwendungsbeispiele und Entwicklungsrichtung

    OpenAIRE

    Dietz, Thomas

    2012-01-01

    Mensch-Roboter-Kollaboration bietet durch Assistenz des Werkers und gleitende Automatisierung neue Möglichkeiten in der Robotertechnik. Durch die direkte Zusammenarbeit von Mensch und Roboter müssen jedoch neue Aspekte in der Gefährdungsbeurteilung betrachtet werden.

  10. Modulation of macrophage functions by heavy metals; Modulation von Makrophagen-Funktionen durch Schwermetalle

    Energy Technology Data Exchange (ETDEWEB)

    Herder, C. [Diabetes-Forschungsinstitut an der Heinrich-Heine-Universitaet Duesseldorf (Germany)

    1999-07-01

    The investigations showed that the LPS/IFN{gamma}-induced production of mediators by bone marrow macrophages was affected more significantly by PbCl{sub 2} in NOD mice, which are prone to autoimmunodiabetes, than in C57BL/6 mice. In NOD mice, the release of TNF{alpha} was enhanced by PbCl{sub 2} while the release of NO, PGE{sub 2}, MCP-1 and IL-12(p40) was decreased. The secretion of IL-12(p70) and IL-10 remained unmodulated. The PbCl{sub 2}-induced modulation of the pattern of secernated mediators can be interpreted as a shift towards an APC1 and maybe even a Th1 phenotype. The reduction of NO production in the presence of PbCl{sub 2} is assumed to be caused by the less pronounced expression of the gene of inducible NO synthase as the volume of specific mRNA measurable by RT-PCR was reduced. The enhanced production of TNF{alpha} is assumed to be due to an effect of PbCl{sub 2} on the protein kinase C dependent signal transducetion pathways. The hypotheses of a contribution of the expression of certain mHC molecules to the reactivity with PbCl{sub 2} could not be validated. The findings illustrate the immunomodulatory properties of the environmental pollutant PbCl{sub 2} and also the particular sensitivity of NOD mice. [German] In der vorliegenden Arbeit konnte gezeigt werden, dass die LPS/IFN{gamma}-induzierte Produktion von Mediatoren durch Knochenmarksmakrophagen aus genetisch zum Autoimmundiabetes praedisponierten NOD-Maeusen deutlich staerker durch Bleichlorid (PbCl{sub 2}) beeinflusst werden kann als bei C57BL/6-Maeusen. Bei NOD-Maeusen wurde die Freisetzung von TNF4{alpha} durch PbCl{sub 2} erhoeht, die Freisetzung von NO, PGE{sub 2}, MCP-1 und IL-12(p40) erniedrigt. Nicht moduliert wurde die Sekretion von IL-12(p70) und IL-10. Die durch PbCl{sub 2} ausgeloeste Modulation des Musters sezernierter Mediatoren kann als Verschiebung in Richtung eines APC1- und in der Folge moeglicherweise auch eines Th1-Phaenotyps gedeutet werden. Die Reduktion der NO

  11. Entgrenzung durch Medien: Internationalisierungsprozesse als Rahmenbedingung der Mediendidaktik

    Directory of Open Access Journals (Sweden)

    Michael Klebl

    2006-07-01

    Full Text Available Die Mediendidaktik ist eine Teildisziplin der Medienpädagogik und hat in den vergangenen Jahren einen enormen Zuwachs an Nachfrage und Bedeutung erfahren. Dieser Bedeutungszuwachs ist bedingt durch den verstärkten Einsatz digitaler Informations- und Kommunikationstechniken in Bildungsprozessen (Stichwort «E- Learning». Der vorliegende Beitrag entwickelt die These, dass die Mediendidaktik ausgehend von medientechnischen Entwicklungen im Kontext von Globalisierung einer Entgrenzung unterworfen ist. Diese Entgrenzung ist Chance und Risiko zugleich. Der vorliegende Beitrag klärt zunächst den Begriff der Entgrenzung. Anhand dreier Phänomene werden Prozesse der Entgrenzung für die Mediendidaktik beschrieben. Ausgehend von der Problematisierung der Prozesse von Entgrenzung werden abschliessend einige Folgerungen für die Mediendidaktik als Teildisziplin der Medienpädagogik zur Diskussion gestellt.

  12. Amerikanisierung durch Internationalisierung: Die Expansion der International Communication Association (ICA

    Directory of Open Access Journals (Sweden)

    Thomas Wiedemann

    2016-12-01

    Full Text Available Basierend auf der Soziologie Bourdieus problematisiert dieser Beitrag die Bemühungen der International Communication Association (ICA, sich über die Öffnung ihrer Führungsetage für Wissenschaftler außerhalb der USA in eine wahrhaft internationale Fachgesellschaft zu verwandeln und der Herausforderung einer global vernetzten Disziplin zu begegnen. Geleistet werden soll so ein kritischer Beitrag zur Selbstreflexion der Kommunikationswissenschaft, zu verstehen als Deutungsangebot und Ausgangspunkt für die wissenschaftliche Diskussion. Die Untersuchung von Habitus und Kapital der 29 ICA-Präsidenten und ICA Fellows aus der internationalen Scientific Community zeigt, dass die weltweit größte kommunikationswissenschaftliche Fachgesellschaft trotz der Ausweitung ihrer Führungsriege immer noch deutlichen US-Einflüssen unterliegt. Die neuen ICAWürdenträger, die für nationale und fachliche Vielfalt stehen sollen, stammen aus Weltregionen, die eine besondere Nähe zu den Vereinigten Staaten auszeichnet, und wurden an US-Universitäten sozialisiert oder stark von der US-amerikanischen Forschungstradition geprägt. Ausnahmen („Einkäufe“ von führenden Vertretern anderer Fachgesellschaften oder alternativer Paradigmen bestätigen die Regel. Die Internationalisierung der ICA-Führungsetage veränderte demzufolge weniger den Machtpol im Fach als vielmehr die Kommunikationswissenschaft weltweit. Zwar gelangten neue Perspektiven ins Zentrum der Disziplin. Im Gegenzug fand jedoch eine Amerikanisierung nationaler Felder statt, allen voran durch ICA Fellows als Vorbilder im Kampf um wissenschaftliches Kapital. Die Bemühungen der ICA, sich durch die Expansion ihrer Führungsriege zu internationalisieren, dürften somit die Machtstrukturen im Fach weiter verfestigt haben.

  13. Israelische Public Diplomacy und ihre Wahrnehmung durch deutsche Journalisten

    Directory of Open Access Journals (Sweden)

    Florian Fleischer

    2014-07-01

    Full Text Available Public Diplomacy stellt insbesondere für Staaten in internationalen Konflikten wie Israel ein wichtiges Kommunikationsinstrument dar, um ausländische Bevölkerungen zu erreichen. Der Artikel zeigt die Ergebnisse einer Studie, in der untersucht wurde, wie die israelische Public Diplomacy von deutschen Journalisten aufgenommen und verarbeitet wird. Journalisten kommt dabei eine Schlüsselrolle als Mittler zwischen dem israelischen Staat und der deutschen Bevölkerung zu. In Anlehnung an den Katalog der Public Diplomacy-Maßnahmen von Gilboa (2006 wurde die Relevanz einzelner Maßnahmen für Journalisten erfragt. Die Ergebnisse zeigen, dass persönliche Kontakte für Journalisten nach wie vor eine wichtige Quelle darstellen und nicht von Cyber-Public Diplomacy ersetzt werden können. Staatliche Public Diplomacy wird zwar wahrgenommen, jedoch wird ihre Objektivität bezweifelt, während NGOs und Stiftungen als vertrauenswürdiger eingestuft werden. Des Weiteren wird Israel in der Öffentlichkeit nach wie vor dominierend mit dem Nahostkonflikt identifiziert, was sich nach Meinung der befragten Journalisten auch in Zukunft nicht durch gezielte Öffentlichkeitsarbeit verhindern lässt.

  14. Development of an early warning system for strong environmental pollution by measuring the activation of defined universally existing stress genes. Final report; Das Auftreten spezifischer Stressproteine bei Umweltbelastung in Chironomiden: Entwicklung eines neuen Fruehwarnsystems fuer starke Umweltbelastung durch Messung der Aktivierung definierter, universell vorkommender Stressgene bei ausgewaehlten Indikatororganismen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Kloetzel, P.M.; Mueller, W.

    1991-12-31

    In the present pilot study we established an ELISA based detection system which allows sensitive monitoring of stress induction as a result of heavy metal pollution. The system is based on the immunological detection of the stress induced hsp70 in larvae of Chironomidae. Using this system it is possible to detect environmental pollution by heavy metals far beyond lethal toxicity values. Since only very few animals are needed the system can also be used in field tests. For poluted isopodes as Oniscus asellus for example a single animal turned out be sufficient to monitor stress induction by Pb{sup +}. The established system ought to serve as basis for a more extended study in which different animals are collected from various polluted environments and tested with respect to already induced hsp synthesis. (orig.) [Deutsch] In der vorliegenden Pilotstudie konnte ein sensitives Nachweissystem fuer die Induktion des ubiquitaeren Stresssystem durch Schwermetalle auf der Basis der immunologischen Detektion von HSP70 etabliert werden. Dieses, auf ELISA Basis arbeitende Testverfahren ermoeglicht es Schwermetallbelastungen weit unterhalb der Toxizitaetsgrenze ueber die Induktion der HSP70 Synthese in Chironomidenlarven nachzuweisen. Die Tatsache, dass nur wenige Tiere fuer eine statistische Aussage benoetigt werden laesst eine Anwendungsmoeglichkeit auch auf Freilandtiere erwarten. So reicht fuer bleibelastete Freiland-Asseln ein einziges Tier zum immunologischen Belastungsnachweis aus. Damit sind die Grundlagen fuer die zweite Phase, die Freilandphase, in welcher sowohl Freilandtiere als auch Gewaesser- und Sedimentproben von verschiedenen Standorten in die Untersuchungen miteinbezogen werden sollen; geschaffen. Es koennen jetzt Tiere aus belasteten Gewaessern bezueglich bereits erfolgter Stressproteinproduktion aber auch auf eine Neuinduzierbarkeit nach zusaetzlicher Laborbelastung untersucht werden. Ebenso koennen Labortiere auf ihre Reaktion gegenueber belasteten

  15. Aktive Strategien zur Schutzzielverletzungerkennung durch eine kontrollierte Machtteilung in der Zugriffskontrollarchitektur

    OpenAIRE

    Abendroth, Jörg

    2004-01-01

    Zugangskontrolle und Intrusion Detection werden oft separat behandelt. Zur Erkennung von Schutzzielverletzungen wird hauptsächlich der Datenverkehr eines Netzwerkes ausgewertet - dies geschieht in einer passiven Weise. Aktive Strategien zum Erkennen von Angriffen sind nur durch neue Zugriffskontrollsysteme und kontrollierte Machtteilung möglich. In dem vorliegenden Beitrag wird eine neue Kategorisierung von Zugriffskontrollsysteme vorgestellt und insbesondere auf die kontrol...

  16. Untersuchungen zur Aktivierung von Transglutaminasen durch das Zytokin Tumor Nekrose Faktor Alpha und Cathepsin D in Fibroblasten und Keratinozyten

    OpenAIRE

    Hellmich, Isabel

    2011-01-01

    Egberts et al. (2004) wiesen eine Aktivitätssteigerung der TG1 in Keratinozytenkulturen durch exogene Zugabe von Cathepsin D (CATD) nach. Daraufhin wurde die Regulation der Transglutaminase 1 (TG1) durch CATD mit Hilfe von CATD defizienten Mausfibroblasten überprüft. Es zeigte sich eine gestörte Prozessierung der TG1 in CATD-defizienten Fibroblasten. Daraus wurde geschlossen, dass die TG1 durch/über CATD vermittelte proteolytische Prozessierung aktiviert werden kann. Da von der Arbeitsgruppe ...

  17. Darstellung hochmolekularer und verzweigter Polyisobutene durch Einsatz von Makroinitiatoren, cyclischen Dienen und Initiatormonomeren

    OpenAIRE

    Schäfer, Marcus

    2007-01-01

    Die Synthese und Charakterisierung von hochmolekularen und verzweigten Polyisobutenen durch Einsatz von neuartigen Makroinitiatoren, cyclischen Dienen und Initiatormonomeren stand im Mittelpunkt dieser Arbeit. Es wurden Lösungsansätze zu aktuellen wissenschaftlichen und technischen Problemen aufgezeigt und miteinander verglichen. Die Kombination von DPE-Technik und kationischer Polymerisation führte zu neuartigen Graftcopolymeren mit variablem Rückgrat und Polyisobutenseitenästen. Die Kombina...

  18. Repetitive transkranielle Magnetstimulation zur Tinnitusbehandlung: keine Verbesserung der Effekte durch dopaminerge Rezeptorstimulation

    OpenAIRE

    Kleinjung, T; Vielsmeier, V; Frank, E; Burger, JS; Landgrabe, M; Hajak, G; Strutz, J; Langguth, B

    2010-01-01

    Einleitung: Die repetitive transkranielle Magnetstimulation (rTMS) stellt ein Instrument zur fokalen Modulation neuronaler Aktivität dar. Niedrigfrequente rTMS über dem auditorischen Kortex wird erfolgreich zur Reduktion des Tinnitusschweregrades eingesetzt. Allerdings sind die positiven Effekte als moderat zu bezeichnen und weisen eine hohe interindividuelle Streuung auf. Untersuchungen an gesunden Probanden konnten zeigen, dass die Effekte von rTMS im Bereich des Motorcortex durch gleichze...

  19. Transparency and efficiency through plant operations management systems; Transparenz und Effizienz durch Betriebsfuehrungssysteme

    Energy Technology Data Exchange (ETDEWEB)

    Ladage, L. [RWE Power AG, Essen (Germany). Informationsmanagement

    2001-04-01

    Plant operations management systems, being IT application systems, provide integral support of the business processes making up plant operations management. The use of plant operations management systems improves mutually interdependent factors, such as high economic performance, high availability, and maximum safety. Since its commissioning in 1988, the Emsland nuclear power station (KKE) has been run with the IBFS plant operations management system. The work flow management system (WfMS), a module of IBFS, is described as an example of job order processing. IBFS-WfMS is to optimize all processes, thus cutting costs and ensuring that processes are run and documented reliably. Assessing the savings effect achieved through the use of IBFS-WfMS clearly reveals the savings in work/time achieved by the system. These savings are quoted as approx. 4 minutes and DM 10, respectively, per working step, which corresponds to several dozens of manyears or several million DM per annum in the KKE plant under consideration. This result can be extrapolated to other plants. (orig.) [German] Betriebsfuehrungssysteme stuetzen als EDV-Anwendungssystem integral die Geschaeftsprozesse der Kraftwerksbetriebsfuehrung. Durch den Einsatz von Betriebsfuehrungssystemen werden die in gegenseitiger Abhaengigkeit befindlichen Faktoren hohe Wirtschaftlichkeit, hohe Verfuegbarkeit und groesstmoegliche Sicherheit im Verbund gefoerdert. Im Kernkraftwerk Emsland (KKE) wird seit Inbetriebnahme der Anlage im Jahr 1988 das Betriebsfuehrungssystem IBFS eingesetzt. Am Beispiel des Workflowmanagementsystems (WfMS), einem Modul des IBFS, wird die Abwicklung von Arbeitsauftraegen dargestellt. Das IBFS-WfMS soll dabei durch Optimierung aller Prozesse sowohl kostensenkend wirken als auch sicherstellen, dass die Prozesse verlaesslich abgewickelt und dokumentiert werden. Eine Abschaetzung des Einspareffektes des IBFS-WfMS zeigt deutlich die durch das System erzielten Ersparnisse an Arbeits-/Zeitaufwand auf

  20. Nitrate removal through combination of nanofiltration and electrocatalysis; Nitratentfernung durch Kombination von Nanofiltration und Elektrokatalyse

    Energy Technology Data Exchange (ETDEWEB)

    Roehricht, M.; Stadlbauer, E.A.; Happel, H. [Fachhochschule Giessen (Germany). Zentrum fuer Umwelttechnik

    1999-07-01

    In a new process combination, nitrate-containing ground water is first of all separated by nanofiltration into a concentrate stream (some 25 %) and a largely nitrate-free permeate (75 %). Then the enriched nitrate in the concentrate is converted into nitrogen by means of electrocatalytic nitrate reduction. Whereas, in nanofiltration, a concentration takes place, electrocatalytic nitrate reduction is a process by which nitrate is converted into elemental nitrogen and, thus, removed. Nanofiltration is a membrane separating process making use of 'open' reverse osmosis membranes, which are characterized by high flow but also reduced retention. (orig.) [German] In einer neuen Verfahrenskombination wird das nitrathaltige Grundwasser zuerst durch Nanofiltration in einen Konzentratstrom (ca. 25%) und ein weitgehend nitratfreies Permeat (75%) aufgeteilt. Im Konzentrat wird dann mittels Elektrokatalytischer Nitratreduktion (EKN) das angereicherte Nitrat zu Stickstoff umgewandelt. Waehrend bei der Nanofiltration eine Aufkonzentrierung erfolgt, wird durch die Elektrokatalytische Nitratreduktion das Nitrat in elementaren Stickstoff umgewandelt und so entfernt. Die Nanofiltration ist ein Membrantrennverfahren, bei dem 'offene' Umkehrosmosemembranen eingesetzt werden, die einen hohen Fluss aber auch eine verminderte Rueckhaltung aufweisen. (orig.)

  1. Charakterisierung von dotierten Zinnoxidschichten für die Detektion von Gasen durch ellipsometrische Messung der Oberflächenplasmonenresonanz

    OpenAIRE

    Fischer, Daniel

    2017-01-01

    In der vorliegenden Dissertation wird die Verbesserung von Gassensoren durch Beschichtung mit dotierten Zinnoxidschichten untersucht, wobei zur Messung der Gase die Methode der oberflächenplasmonenresonanzverstärkten Ellipsometrie (SPREE) angewendet wurde. Die vorgestellten Ergebnisse unterteilen sich dabei in drei Schritte: Herstellung der Schichtsysteme, Analyse der chemischen, strukturellen und optischen Eigenschaften, sowie die Anwendung in der SPREE Gasmessung. Die dotierten Zinnoxid...

  2. Value-added services through professional innovation management; Mehrwert durch professionelles Innovationsmanagement

    Energy Technology Data Exchange (ETDEWEB)

    Niemeier, D. [Accenture GmbH, Frankfurt am Main (Germany); Scholtissek, S.

    2001-02-01

    Competition in the deregulated internal market in electricity has changed this market into a commodity market. All traders in the energy sector today are looking for innovative marketing and sales strategies linked to the product 'electric power' and the related services. Cooperation within the framework of a multi-utility enterprise is a key example in this context. The article discusses successful business management strategies. (orig./CB) [German] Wettbewerb hat den Energiemarkt in einen Commodity-Markt verwandelt. Alle Energieversorger arbeiten heute an den gleichen Ideen wie energienahe Dienstleistungen, Innovationsbeispiel Multi Utility, Powerline Communication oder dezentrale Erzeugung. Damit diese Produkte schnell dem Kunden zur Verfuegung stehen, bedarf es einer Professionalisierung des Innovationsmanagements durch klar definierte Strategien, kreatives organisatorisches und effizientes Wissensmanagement sowie flexible Start-up-Strukturen. (orig./CB)

  3. Carry-over of trace pollutants through fog on forests; Eintrag von Spurenstoffen durch Nebel auf Waelder

    Energy Technology Data Exchange (ETDEWEB)

    Winkler, P. [Deutscher Wetterdienst, Meteorologisches Observatorium, Hamburg (Germany); Pahl, S. [Deutscher Wetterdienst, Meteorologisches Observatorium, Hamburg (Germany)

    1993-11-01

    Deposition of pollutants contained in fog to conifers is one important path of carry-over apart from wet deposition. In order to estimate deposition of pollutants from cloud water to conifer forests the liquid water content was registered at the Feldberg/Black Forest over a period of 21 months. A new sight- LWC-relation could be parametrised. With these parameters and the measured values of pollutants contained in fogs we calculated deposition of water quantities at several mountain stations. Fog water samples show higher concentration of pollutants than rainfall. Over a whole year concentrations of sulfate and nitrate when calculated for a uniform liquid water content show a peak in May. Comparative calculations of the flow of cloud water and contents on a forest of conifers were carried out for the period 1982-1991. In higher mountaneous regions deposition of trace substances though fog to needle trees was to be found to be as high as from rainfall. (orig./EW) [Deutsch] Die Stoffdeposition durch Nebelinterzeption auf Nadelbaeume stellt einen bedeutenden Eintragspfad neben der nassen Deposition dar. Zur Abschaetzung der Stoffdeposition durch Wolkentropfen auf Nadelwaelder wurde der Fluessigwassergehalt auf dem Feldberg/Schwarzwald ueber 21 Monate registriert. Aus diesen Daten wurde eine neue Sicht-LWC-Beziehung parametrisiert. Mit Hilfe dieser Parametrisierung und mit den Messwerten von Nebelinhaltsstoffen wurden Depositionsrechnungen der Wassermenge fuer mehrere Bergstationen durchgefuehrt. In Nebelwasserproben weisen die Inhaltsstoffe hoehere Konzentrationen auf als im Niederschlag. Im Jahresgang weisen die auf einen einheitlichen Fluessigwassergehalt umgerechneten Konzentrationen von Sulfat und Nitrat ein Maximum im Mai auf. Vergleichsrechnungen zum Fluss von Wolkenwasser und Inhaltsstoffen auf einen Nadelwald wurden fuer den Zeitraum 1982 - 1991 durchgefuehrt. Fuer die Hochlagen der Mittelgebirge ist die Deposition von Spurenstoffen durch Nebelinterzeption an

  4. Objektverfolgung durch Fusion von Radar- und Monokameradaten auf Merkmalsebene für zukünftige Fahrerassistenzsysteme

    OpenAIRE

    Liu, Feng

    2010-01-01

    Die vorliegende Arbeit beschreibt eine neuartige Objektverfolgung durch Fusion von Radar- und Monokameradaten auf Merkmalsebene. Dabei werden zuerst die statistischen Fehlermodelle der Sensoren analysiert. Anschließend wird ein neues Assoziationsverfahren auf Basis der PDA-Methodik untersucht. Der Schwerpunkt dieser Arbeit ist die Behandlung der Objektdynmaik mit Hilfe eines adaptiven IMM-Filters. Schließlich wird die Thematik Objektklassifikation sowie Gassenbreiteschätzung beleuchtet.

  5. Mechatronics in the car: success through efficient methodological development; Mechatronik im Kraftfahrzeug: Erfolg durch effiziente Entwicklungsmethodik

    Energy Technology Data Exchange (ETDEWEB)

    Dieterle, W.; Dilger, E. [Bosch (R.) GmbH, Gerlingen (Germany). Zentralbereich Forschung und Vorausentwicklung; Buerger, K.G. [Bosch (R.) GmbH (Germany)

    2001-04-01

    The ever increasing requirements with respect to fuel consumption, emissions as well as safety and comfort necessitate ever increasing complex functionalities in the car. They are realized, as a rule, by a combination of mechanics, hydraulics, electrics/electronics and, more and more, software. In the past, the development of the various electric/electronic, mechanical or hydraulic components tended to happen at separate levels, nowadays the focus is more on a comprehensive approach to the overall system. In this context, the integrated development of 'mechatronic' components, including the respective software, leads to a targeted solution. (orig.) [German] Staendig steigende Anforderungen hinsichtlich Kraftstoffverbrauch, Emissionen sowie Sicherheit und Komfort bedingen immer komplexere Funktionalitaeten im Kraftfahrzeug. Sie werden in der Regel durch eine Kombination von Mechanik, Hydraulik, Elektrik/Elektronik und zunehmend auch Software realisiert. Nachdem in der Vergangenheit die Entwicklung meist noch auf getrennten Ebenen verlief, steht heute ein uebergreifender, das Gesamtsystem umfassender Ansatz im Vordergrund. Dieser Beitrag der Robert Bosch GmbH berichtet ueber aktuelle Mechatronik-Entwicklungen. Dabei sorgt die integrierte Entwicklung der 'mechatronischen' Komponenten unter Einbezug der zugehoerigen Software fuer eine zielgerichtete Loesung. (orig.)

  6. Processing of maize plants by rotary kiln pyrolysis; Veredlung von Maispflanzen durch Pyrolyse im Drehrohrreaktor

    Energy Technology Data Exchange (ETDEWEB)

    Klose, W.; Wiest, W. [Kassel Univ. (Gesamthochschule) (Germany). Inst. fuer Thermische Energietechnik

    1996-12-31

    The fuel quality of maize plants is to be characterized by short, elementary and thermonalysis. The plants will be pyrolyzed in order to facilitate transport and storage. The formal kinetic parameters of three parallel reactions describing solid matter decomposition are defined by means of TG-DSC, and the reaction enthalpy is measured. Pyrolysis experiments in a rotary kiln converter in the kg range show a strong dependence of the product spectrum on process temperature. In particular, the pyrolysis gas yield increases with temperature at the expense of pyrolysis oil and water. (orig) [Deutsch] Zur energetischen Nutzung von Kulturpflanzen wird die brennstofftechnische Charakterisierung von Maispflanzen durch Kurz-, Elementar- und Thermoanalyse durchgefuehrt. Zur Reduzierung des Aufwands fuer Transport und Lagerung sollen die Pflanzen pyrolysiert weren. Mit Hilfe der TG-DSC werden formalkinetische Parameter von drei Parallelreaktionen zur Beschreibung der Feststoffzersetzung bestimmt und die Reaktionsenthalpie gemessen. Pyrolyseversuche in einem Drehrohrreaktor im Kilogramm-Massstab ergeben eine starke Abhaengigkeit des Produktspektrums von der Prozesstemperatur. Insbesondere steigt die Pyrolysegasausbeute auf Kosten der Bildung von Pyrolyseoel und Wasser mit der Temperatur stark an. (orig)

  7. Optimization of sewage treatment plants through adjustment of the acid capacity; Optimierung von Klaeranlagen durch Saeurekapazitaetseinstellung

    Energy Technology Data Exchange (ETDEWEB)

    Herbst, B.; Soelter, K. [Bundesverband der Deutschen Kalkindustrie e.V., Koeln (Germany)

    1999-07-01

    In many areas with soft water (low water hardness), variations of degradation behaviour are noted in the biological cleaning of municipal and industrial waste water. The use of acidic precipitation agents and processes of biological ammonium oxidation (nitrification) reduce the acid capacity of water. Addition of lime in different forms can prevent this drop in acid capacity. As a new development in this field, lime hydrate can be added in dry form. This form of application dispenses with some of the elements normally installed at sewage treatment plants. Several examples demonstrate the advantages offered by this technique, especially the need for less metal salts to eliminate phosphate. (orig.) [German] In vielen Weichwassergebieten (geringer Wasserhaerte) treten bei der biologischen Reinigung von kommunalen und industriellen Abwaessern Schwankungen des Abbauverhaltens auf. Der Einsatz von sauren Faellmitteln und die Prozesse der biologischen Ammoniumoxidation (Nitrifikation) senken die Saeurekapazitaet des Wassers ab. Durch die Zugabe von Kalk in verschiedenen Dosierformen kann die Saeurekapazitaetsabsenkung verhindert werden. Eine neue Entwicklung auf diesem Gebiet ist die Trockendosierung von Kalkhydrat. Wesentliche Teile der sonst installierten Anlagentechnik sind bei dieser Art der Dosierung nicht mehr notwendig. Anhand von mehreren Beispielen wird erlaeutert, welche Vorteile dieses Verfahren bieten kann, insbesondere bei der Einsparung von Metallsalzen zur Phosphatentfernung. (orig.)

  8. Wird die Inzidenz von Lymphknotenmetastasen durch Sentinel-Lymphknoten-Dissektion beim Prostatakarzinom erhöht?

    Directory of Open Access Journals (Sweden)

    Schafhauser W

    2006-01-01

    Full Text Available Fragestellung: Eine pelvine Lymphknotenmetastasierung ist von entscheidender Bedeutung für die Tumorprogression beim Prostatakarzinom. Unser Ziel war es festzustellen, ob die "Sentinel-Lymph-Node-Dissektion" (SLND im Rahmen der radikalen retropubischen Prostatektomie (RRP im klinischen Routinebetrieb machbar ist und ob durch sie ein höherer Prozentsatz an identifizierten und entfernten Lymphknotenmetastasen resultiert als bei der Standardlymphdissektion. Methode: Von September 2002 bis November 2004 wurde bei 162 Patienten (67 Jahre mit Prostatakarzinom ohne Hinweis auf Metastasierung am Vortag der RRP in jeden Prostatalappen 0,6 ml 99mTechnetium-Nanokolloid (max. 150 MBq transrektal ultraschallgesteuert appliziert. Anschließend wurde eine Lymphszintigraphie durchgeführt. Intraoperativ wurden neben der üblichen begrenzten pelvinen Lymphdissektion zusätzlich die szintigraphisch markierten Lymphknoten (SLN mittels Gammasonde (C-Trak aufgesucht und gezielt entfernt. Ergebnisse: Eine SLND war bei 161 Patienten möglich. 32 Patienten (19,8 % zeigten Lymphknotenmetastasen. 39 % der Lymphknotenmetastasen fanden sich außerhalb des Standarddissektionsgebietes von Vasa iliaca externa und Fossa obturatoria. Schlußfolgerung: Die SLND im Rahmen einer RRP läßt sich mit akzeptablem Aufwand in den Routinebetrieb einer urologischen Klinik bei enger Kooperation mit Nuklearmediziner und Pathologen integrieren. Die Rate an identifizierten Lymphknotenmetastasen wird im Vergleich zur üblichen begrenzten pelvinen Lymphdissektion ohne SLN-Markierung deutlich erhöht.

  9. Continuous high-temperature recycling of waste by the Thermoselect process; Unterbrechungsloses Hochtemperaturrecycling - Abfallveredelung durch Thermoselect

    Energy Technology Data Exchange (ETDEWEB)

    Stahlberg, R. [Thermoselect Engineering S.r.l., Verbania (Italy)

    1998-09-01

    Based on the experience with the Thermoselect plant at Fondotoce, Italy (100 Mg/d), which was commissioned in 1992, a 225,000 Mg/a plant with three units (720 Mg/d) is being constructed at Karlsruhe, Germany. The plant is scheduled for commissioning in 1998. The contribution presents the product balance, the approved emission levels, and the energy uses envisaged for the Karlsruhe project. Final product quality data and energy yields illustrate the differences from conventional thermal processes. An ecobalance comparing the Thermoselect process with grate furnaces shows that water, soil and air pollution is much reduced by the new Termoselect process. Apart from high product quality, the plant also has the advantage of reduced dumping cost and no long-term ecological hazards. (orig./SR) [Deutsch] Auf Basis der grosstechnischen Erfahrung mit einer seit 1992 in Fondotoce (Italien) betriebenen THERMOSELECT-Anlage (Kapazitaet 100 Mg/d), Restmuell umweltgerecht zu behandeln, wird 1998 der Aufbau einer 225.000 Mg/a-Anlage mit 3 Linien (720 Mg/d) in Karlsruhe (Deutschland) abgeschlossen. Die Produktebilanz und die genehmigten Emissionswerte sind in dem Artikel belegt. Gezeigt wird die fuer das Projekt Karlsruhe vorgesehene Energienutzung. Die Endprodduktqualitaeten und die Energieausbeute verdeutlichen die Unterschiede zu bisherigen konventionellen thermischen Verfahren. Eine erarbeitete Oekobilanz mit einem Vergleich von THERMOSELECT und Rostfeuerung zeigt die deutlich geringe Belastung der Umweltkompartimente Wasser, Boden und Luft durch das neue THERMOSELECT-Verfahren. Neben hohen Produktqualitaeten wird - verglichen mit traditionellen Techniken - ein deutlich verringerter Entsorgungspreis erreicht, wobei gleichzeitig oekologische Belastungen auszuschliessen sind. (orig./SR)

  10. Biosorption of heavy metals by immobilized microalgae; Biosorption von Schwermetallen durch immobilisierte Mikroalgen

    Energy Technology Data Exchange (ETDEWEB)

    Wilke, A.; Bunke, G.; Goetz, P.; Buchholz, R. [Technische Univ. Berlin (Germany). Fachgebiet Bioverfahrenstechnik

    1999-07-01

    . Durch geeignete Versuche muessen die verschiedenen Stofftransportwiderstaende (Filmwiderstand, Diffusionswiderstand im Innern der Partikel) voneinander entkoppelt werden, damit eine Bestimmung derselben ermoeglicht wird. (orig.)

  11. Pollution and pollution tolerance in the case of polycyclic aromatic hydrocarbons (PAH); Belastung durch Polyzyklische aromatische Kohlenwasserstoffe (PAK)

    Energy Technology Data Exchange (ETDEWEB)

    Renger, M.; Mekiffer, B. [Technische Univ. Berlin (Germany). Inst. fuer Oekologie-Bodenkunde

    1997-12-31

    The purpose of the present follow-up project was to examine the contamination with polycyclic aromatic hydrocarbons (PAH) of different anthropogenic urban soils including clay soils containing demolition waste, household waste, ash, and residues from a coking plant. A further task was to analyse, or infer from other part-projects, standard soil parameters such as organic carbon content, pH, and anion levels in order to clarify any relationships between PAH contamination and the more easily determinable soil characteristics. Furthermore, the sorption behaviour for PAH of selected anthropogenic urban soils was to be characterised by means of batch experiments. [Deutsch] Im Rahmen des Anschlussvorhabens sollte die Kontamination von anthropogenen Stadtboeden- darunter Truemmerschutt-, Hausmuell-, Asche- sowie Kokereilehmboden- durch polyzyklische aromatische Kohlenwasserstoffe (PAK) untersucht werden. Zusaetzlich sollten die bodenkundlichen Standardparameter Corg, pH-Wert, Anionengehalte und KAKpot analysiert bzw. von den anderen Teilvorhaben uebernommen werden, um Zusammenhaenge zwischen der PAK-Kontamination und relativ leicht zu bestimmenden bodenkundlichen Kennwerten klaeren zu koennen. Das Sorptionsverhalten ausgewaehlter anthropogener Stadtboeden fuer PAK sollte durch Batchversuche charakterisiert werden. (orig./SR)

  12. Energy saving by hot operating bearings in high temperature kilns. Final report; Energieeinsparung durch Heisslager in Hochtemperaturanlagen. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Maier, H.R.

    2003-07-01

    The project intended to show that ceramic sliding bearings will ensure longer life, energy and cost savings, reduced environmental pollution, and functional improvements. Ceramic sliding bearings were tested at high temperatures of up to 1400 C and low sliding rates, with and without lubrication. High-temperature test stands were constructed in the two partner institutes and adapted to the specifications of the industrial partners concerning the parameters to be investigated. (orig.) [German] Ziel des Forschungsvorhabens 'Energieeinsparung durch Heisslager in Hochtemperaturanlagen' (EHIH) ist nachzuweisen, dass durch den Einsatz von keramischen Gleitlagern im Hochtemperaturbereich Standzeiterhoehungen, Energie- und Kosteneinsparungen, Umweltentlastungen und Funktionsverbesserungen moeglich sind. Auf der Basis der Anforderungsprofile der Industrie- und Forschungspartner waren umfangreiche Verschleisstests keramischer Gleitlager bei hohen Temperaturen (bis 1400 C) sowie niedrigen Gleitgeschwindigkeiten ohne und mit Feststoff-Schmierung vorgesehen. Hierzu wurden an beiden beteiligten Instituten Hochtemperatur-Pruefstaende aufgebaut, die den Anforderungen der jeweiligen Industriepartner hinsichtlich der zu testenden Lagerparameter (Drehzahl, Flaechenpressung, Temperaturprofile, etc.) angepasst wurden. (orig.)

  13. Artifacts in MR imaging due to ventilation tubes; MR-Bildartefakte durch Anaesthesie-Beatmungsschlaeuche

    Energy Technology Data Exchange (ETDEWEB)

    Kugel, H.; Zaro Weber, O.; Krug, B. [Koeln Univ. (Germany). Inst. und Poliklinik fuer Radiologische Diagnostik; Steppan, G. [Draeger Medizintechnik GmbH, Luebeck (Germany)

    2001-10-01

    Purpose: MR examinations may be necessary in patients under artificial ventilation. Possible imaging artifacts originating from ventilation tubing made from silicone are demonstrated. Materials and Methods: In silicone carbohydrate residues are bound to silicon atoms. In silicone gels they give strong signals in MR imaging. MR signals from more solid silicone rubber are only possible if the carbohydrate residues in the molecular chain retain a sufficient mobility. To investigate whether silicone tubes give signals in MR images, different tubes for ventilation made from silicone and polyester were examined at 1.5 T (Gyroscan ACS NT, Powertrak 6000 gradients) with MR imaging and spectroscopy (MRS: PRESS, TE = 20 ms; relaxation time measurements: MIXED sequence; imaging: FFE [gradient echo] and TSE sequences). Results: Using short TE ({<=} 4 ms), tubes and sockets made from silicone could be imaged with FFE and SE sequences. An SE sequence with a short TE = 4 ms provided a sufficient signal intensity to depict the tube clearly besides brain tissue of a volunteer. When foldover occured, the signal of the tube was projected onto the brain tissue. MR spectra confirmed that the image signal originated from the carbohydrate residues of the silicon molecule chains, not from residual water in the tube wall material. T{sub 2} determinations indicate a multiexponential relaxation including a large component with a short TE ({<=} 15 ms). Tubes made from a polyester elastomere did not show up in MR images. Conclusion: In MR images acquired with very short echo times silicone in ventilation tubes may produce significant artifacts. (orig.) [German] Ziel: Untersuchungen am MR-Tomographen sind unter Umstaenden auch bei beatmeten Patienten indiziert. Auf die moeglichen Bildartefakte durch Beatmungsschlaeuche aus Silikon soll hier hingewiesen werden. Material und Methodik: In Silikonen sind Kohlenwasserstoffreste an Silizium-Atome gebunden. Silikongel zeigt deutliche Signale im MR

  14. Gene

    Data.gov (United States)

    U.S. Department of Health & Human Services — Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes,...

  15. Contrast generation in the nuclear-spin tomography by pulsed ultrasound; Kontrasterzeugung in der Kernspintomographie durch gepulsten Ultraschall

    Energy Technology Data Exchange (ETDEWEB)

    Oehms, Ole Benjamin

    2009-07-10

    In the framework of this thesis a combined method of ultrasound and nuclear-spin tomography is presented. Via ultrasound pulses by the sound-radiation force in liquids and tissue phantoms motions are generated, which depend on ther viscoelastic properties. This motions are made visible by a motion-sensitive tomograph sequence in the phase image of the tomograph in form of a phase change. The first measurements on simple phantoms and liquids are presented. [German] Im Rahmen dieser Arbeit wird eine kombinierte Methode aus Ultraschall und Kernspintomographie vorgestellt. Ueber Ultraschallpulse werden durch die Schallstrahlungskraft in Fluessigkeiten und Gewebephantomen Bewegungen erzeugt, die von den viskoelastischen Eigenschaften abhaengen. Diese Bewegungen werden mit einer bewegungssensitiven Tomographensequenz im Phasenbild des Tomographen in Form einer Phasenaenderung sichtbar gemacht. Die ersten Messungen an einfachen Phantomen und Fluessigkeiten werden praesentiert. (orig.)

  16. Bridging of shrinkage gaps by Exterior Thermal Insulation Composite Systems (ETICS); Zur Frage der Ueberbrueckung von Bewegungsfugen durch Waermedaemmverbundsysteme

    Energy Technology Data Exchange (ETDEWEB)

    Kuenzel, H. [Fraunhofer-Institut fuer Bauphysik, Holzkirchen (Germany)

    1998-10-01

    The bridging performance in respect to shrinkage gaps of ETICS is a subject of controversy. Practitioners point out good experience, calculative examinations, however, support the requirement of special tests prior to technical approval. Experiments at a half-timbered facade where an ETICS was applied to dry-out the wall confirm their bridging ability during the shrinkage of the wooden studs. Therefore a special bridging test is generally not necessary for an insulation thickness of 80 mm or more. (orig.) [Deutsch] Zur Frage der Ueberbrueckung von Bewegungsfugen durch Waermedaemmverbundsysteme gibt es unterschiedliche Meinungen: Die Anwender weisen auf gute Erfahrungen in der Praxis hin, rechnerische Untersuchungen lassen hingegen eine spezielle Ueberpruefung fuer zweckmaessig erscheinen, die bisher bei der Zulassung solcher Systeme gefordert worden ist. Untersuchungen an einem Fachwerk-Versuchshaus mit nachtraeglich aufgebrachtem Waermedaemmverbundsystem bestaetigen dessen fugenueberbrueckende Eigenschaft beim Schwinden des Fachwerkholzes. Demnach ist bei Daemmschichtdicken von mindestens 80 mm ein Nachweis der Fugenueberbrueckungs-Faehigkeit generell nicht erforderlich. (orig.)

  17. Verbesserung der Lebensqualität bei Kinderwunsch-Behandlung durch den Gebrauch patientenfreundlicher Follitropin-Fertigpens

    Directory of Open Access Journals (Sweden)

    Bitzer J

    2011-01-01

    Full Text Available Zur Behandlung des unerfüllten Kinderwunsches unterziehen sich viele Frauen einer assistierten Reproduktionstherapie mit ovarieller Stimulation. Dazu erfolgen tägliche subkutane Injektionen mit Gonadotropinen, welche die Patientinnen eigenständig durchführen können. Häufig haben Patientinnen dabei Angst vor Spritzen und injektionsbedingten Schmerzen. Die vorliegende Beobachtungsstudie zum Einfluss der Anwendung des Follitropin alfa-Fertigpens (Gonal-f®-Pen auf die Lebensqualität der Patientinnen zeigt die Ergebnisse einer Befragung von 170 Frauen zu Angst und Zufriedenheit mit diesem Applikationssystem. Die Erfahrungen wurden vor und nach den Behandlungszyklen auf numerischen Analogskalen quantifiziert und nachfolgend verglichen. Die Studie ergab, dass durch die Verwendung des Gonal-f®-Pens die Angst vor der Injektion und vor Schmerzen signifikant abnahm. Zudem waren die Patientinnen mit dem Applikationssystem äußerst zufrieden.

  18. Konstruktion, Aufbau und Erprobung einer Einzelionen-Bestrahlungsapparatur und Untersuchung der Inaktivierung von Sporen von bacillus subtilis durch einzelne sehr schwere Ionen (Z $>$ 27) in Abhängigkeit vom Stossparameter

    CERN Document Server

    Weisbrod, U

    1988-01-01

    Konstruktion, Aufbau und Erprobung einer Einzelionen-Bestrahlungsapparatur und Untersuchung der Inaktivierung von Sporen von bacillus subtilis durch einzelne sehr schwere Ionen (Z $>$ 27) in Abhängigkeit vom Stossparameter

  19. Stufenweise Ionisation positiv geladener Kohlenstoffund Stickstoffionen durch Elektronenstoss ($C^{2+} \\to C^{3+} \\to C^{4+} \\to C^{5+} \\to C^{6+}$ und $N^{3+} \\to N^{4+} \\to N^{5+} \\to N^{6+} \\to N^{7+}$)

    CERN Document Server

    Donets, E D; Donets, E D no 1; Ilyushchenko, V I no 1

    1975-01-01

    Stufenweise Ionisation positiv geladener Kohlenstoffund Stickstoffionen durch Elektronenstoss ($C^{2+} \\to C^{3+} \\to C^{4+} \\to C^{5+} \\to C^{6+}$ und $N^{3+} \\to N^{4+} \\to N^{5+} \\to N^{6+} \\to N^{7+}$)

  20. Strategic alliances between utilities and technical service providers - a means to generate value added; (Mehr-)Wert durch strategische Partnerschaften zwischen EVU und technischen Dienstleistern. Vertrauensbildung erforderlich

    Energy Technology Data Exchange (ETDEWEB)

    Gross, M.; Handschuh, M.; Hartmann, B.; Neumann, D.; Weiss, M. [A.T. Kearney Management Consultants Frankfurt (Main), Berlin, Stuttgart (Germany)

    2003-04-07

    In order to forge strategic alliances between utilities and technical service providers successfully, the most critical success factors must be understood by both partners. Above all, the value added must be clearly defined and quantified. Only fair and accepted business models and profit sharing agreements generate stable partnerships. Comprehensive communication ensures the necessary acceptance by all relevant stakeholders. Rigorous project management leads to effective and efficient initiation and implementation of the alliance. (orig.) [German] Fuer die erfolgreiche Realisierung von strategischen Partnerschaften zwischen Energieunternehmen und technischen Dienstleistern muessen die wesentlichen kritischen Erfolgsdimensionen von beiden Partnern verstanden werden. Diese sind vor allem eine quantifizierte Darstellung des Nutzens durch eindeutig definierte Verbesserungsansaetze sowie eine nachvollziehbare Logik des Geschaeftsmodells. Eine umfassende Kommunikation sichert die notwendige Akzeptanz aller relevanten Stakeholder. Durch eine straffe Vorgehensweise kann der Partnerschaftsanbahnungs- und -umsetzungsprozesses effizient gesteuert und im Ergebnis eine signifikante Kosten- und Ertragsoptimierung fuer realisiert werden. (orig.)

  1. [Peter von Brackel. Christlich Gesprech von der grawsamen Zerstörung in Lifland durch den Muscowiter vom 58. Jar her geschehenn...] / Stefan Donecker

    Index Scriptorium Estoniae

    Donecker, Stefan, 1977-

    2015-01-01

    Arvustus: Christlich Gesprech von der grawsamen Zerstörung in Lifland durch den Muscowiter vom 58. Jar her geschehenn: auch ihren Ursachen mit einer kurtzen Predig und Vermanung, wie beid, Gotlosenn unnd Frommen, diese schreckliche Mutation fruchtbarlich behertzigen und ihnen zu Nutz machen sollen: durch Timannum Brakel Livoniensem, der Gemeine Christi vonn der Augsburgischen Confession Prediger zu Anttorf einfeltig gestellet unnd inn Druck verfertiget = Darstellung der Geschichte Livlands vor und während des "Livländischen Krieges" bis 1578 in gereimter Dialogform und Prosa von dem Prediger und Geschichtsschreiber Timann Brakel : Originaltext und Übersetzung ins Hochdeutsche mit Kommentaren, Ergänzungen, Bildern und dem Lebenslauf des Timann Brakel. (Beiträge zur baltischen Geschichte, 19). Verlag Harro von Hirschheydt. Wedemark 2012

  2. Climate protection by reducing the emissions of greenhouse gases in households and the tertiary sector through climate-conscious behaviour. Vol. 1; Klimaschutz durch Minderung von Treibhausgasemissionen im Bereich Haushalte und Kleinverbrauch durch klimagerechtes Verhalten. Bd. 1. Private Haushalte

    Energy Technology Data Exchange (ETDEWEB)

    Brohmann, B.; Cames, M.

    2000-06-01

    The aim of the project was to identify areas in households and the tertiary sector in which changes in behaviour could result in energy conservation and thus a reduction of greenhouse gas emissions, and to quantify the potentials for 1995, 2005 and 2020. A second focus was on the analysis and evaluation of programmes and instruments to realise the potentials. With literature evaluation, expert interviews, and a household servey potentials and further technical development have been identified. In sum, behavioural measures can contribute to the CO2 reduction by 64 million tons in 1995 in households and 27 in the commercial sector in which the potential decreases to 18 million tons in 2020 due to the autonomous technical development. Adequate promotion programmes can help to realise 20-30% of the potential by 2020. (orig.) [German] Ziel des Vorhabens war, im Sektor private Haushalte und Kleinverbrauch Bereiche zu identifizieren, in denen Verhaltensaenderungen zur Energieeinsparung fuehren koennen, und diese Potenziale fuer 1995, 2005 und 2020 zu quantifizieren. Darauf aufbauend waren Programme und Instrumente zur Umsetzung aufzuzeigen und zu bewerten. Gestuetzt auf Literaturrecherchen und Expertengespraeche wurden Einzelpotenziale, Rahmenbedingungen, Entwicklungstrends in der Technik und im Ausstattungsgrad ermittelt. Insgesamt koennten Verhaltensmassnahmen im Haushaltssektor die CO2-Emissionen im Basisjahr 1995 um 64 Mio, im Kleinverbrauch um 27 Mio t vermindern. Bis 2020 bleibt dieses Potenzial im Haushaltssektor in etwa gleich. Im Kleinverbrauch sinkt es infolge der autonomen Technikentwicklung auf 18 Mio t ab. Durch geeignete Programme koennen bis 2020 etwa 20-30% des Potenzials erreicht werden. (orig.)

  3. Regenerierung fester Katalysatoren für die Isobutan-Buten-Alkylierung durch hydrierende Behandlung in flüssiger Phase

    OpenAIRE

    Klingmann, Raoul

    2005-01-01

    In der vorliegenden Arbeit wurde die Regenerierung fester Katalysatoren für die Isobutan/Buten-Alkylierung durch hydrierende Behandlung in flüssiger Phase untersucht. Als Katalysatoren dienten edelmetallhaltige Zeolithe des Typs La-X (FAU). Die Alkylierungs- und Regenerierungsexperimente wurden in einer Apparatur mit kontinuierlich betriebenem Rührkesselreaktor und Analytikeinheit mit On-line-Probenahmevorrichtung durchgeführt. Zunächst wurde der Aufbau der Koksdeposite in Abhängigkeit von...

  4. ERWERB VON EIGENTUMSRECHTEN DURCH ERSITZUNG VON IMMOBILIEN IN SOZIALEM EIGENTUM IM KONTEXT DER NEUEN PRAXIS DES EUROPÄISCHEN GERICHTSHOFS FÜR MENSCHENRECHTE

    Directory of Open Access Journals (Sweden)

    Damir Kontrec

    2017-01-01

    Full Text Available Dieser Artikel befasst sich mit der Ersitzung von Immobilien als einer Art des Erwerbs des Eigentumsrecht im gesellschaftlichen Eigentum. Er weist auf drei wichtige Entscheidungen des Europäischen Gerichtshofs für Menschenrechte gegen die Republik Kroatien hin, die einen direkten Einfl uss auf das Rechtsdenken der ordentlichen Gerichte in der Republik Kroatien in Bezug auf den Erwerb von Eigentumsrecht durch Ersitzung von Immobilien in sozialem Eigentum haben.

  5. Untersuchungen zur Anwendbarkeit und Validität von In-vitro-Methoden bezüglich der Inhibition von Cytochrom-P450-Enzymen durch Arzneipflanzenextrakte

    OpenAIRE

    Frank, Andreas

    2009-01-01

    Um Arzneimittelwechselwirkungen von Arzneistoffen sowie neuen Arzneistoffkandidaten zu vermeiden, werden zur Abschätzung des Interaktionsrisikos so genannte In-vitro-Interaktionsstudien durchgeführt. Hierfür werden vor allem Mikrotiterplatten-basierte Fluoreszenz- und LC/MS-Methoden verwendet. Das Ziel dieser Arbeit war die Untersuchung der Anwendbarkeit und Validität dieser In-vitro-Methoden bezüglich der Inhibition von CYP-Enzymen durch Arzneipflanzenextrakte. Die in dieser Arbeit erhaltene...

  6. Expression und Funktion Apoptose modulierender Gene von Orthopockenviren

    OpenAIRE

    Witkowski, Peter

    2010-01-01

    Dipl.-Ing. Peter Witkowski Abstract zur Dissertation „Expression und Funktion Apoptose regulierender Gene von Orthopockenviren“ Nach der Eradikation des humanpathogenen Variolavirus durch das WHO-Impfprogramm im 20. Jahrhundert treten andere Orthopockenviren (OPV) gehäuft als Erreger humaner Infektionen in Erscheinung. Weniger gefährlich als die humanen Pocken, können Kuhpocken- oder Vacciniaviren dennoch Menschen infizieren und zu schweren Komplikationen führen. OPV besitzen Erbinformationen...

  7. Replacement of coal-fuelled stoves by modern room heating systems in multistorey dwellings; Abloesung der Kohle-Einzelofenheizung durch moderne Raumheizungssysteme in Mehrgeschossbauten

    Energy Technology Data Exchange (ETDEWEB)

    Lindner, K. [Institut fuer Energetik und Umwelt gGmbH, Leipzig (Germany)

    1997-12-31

    In eastern Germany, approximately one million residential units in multi-storey apartment buildings are still heated with coal-fired stoves. Not all building societies or private house-owners can afford to substitute modern heating systems for individual coal stoves within the foreseeable future. Problems encountered when modernizing heating systems are described. A way of pre-financing modernization is by loans given by the tenants to the building society. The paper deals with individual aspects, such as contract conditions, organization and experience with this model, as well as its advantages for the landlord and tenant. (MSK) [Deutsch] In Ostdeutschland werden noch rund 1 Million Wohnungen in mehrgeschossigen Wohnhaeuern mit Kohle geheizt. Nicht alle Wohnungsgenossenschaften oder privaten Hauseigentuemer sind finanziell in der Lage in absehbarer Zeit die Kohle-Einzelheizung durch moderne Heizungssysteme abzuloesen. Im Folgenden werden die Probleme bei der Heizungsmodernisierung beschrieben. Der Weg Heizungsmodernisierung durch Mieterdarlehen wird in einzelnen Punkten wie Finanzierung durch Mieterdarlehen, deren Organisation und Erfahrungen mit diesem Modell sowie die Vorteile fuer Vermieter und Mieter dargelegt.

  8. Altitude dependence of trace substance deposition from clouds to forests. Final report; Hoehenabhaengigkeit der Spurenstoffdeposition durch Wolken auf Waelder. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Pahl, S.; Winkler, P.

    1995-12-31

    Novel forest decline is particularly pronounced in the area of the ridges of medium-range mountains. Whereas acid precipitation was viewed as its sole cause early on in the discussions, it turned out later that the impact of trace gases, too, contributes to the damaging of forests. This report wants to point out the importance of fog interception, which equally plays a part in the pollutant receipts of forests. The deposition of fog water to a forest stand depends very much on altitude, so that trace substance deposition, too, is to be expected to be dependent on altitude. By attempting to quantify this effect, the report helps to pinpoint areas of relevance of this deposition pathway (orig./KW) [Deutsch] Die neuartigen Waldschaeden sind in den Kammlagen der Mittelgebirge besonders ausgepraegt. Waehrend in der anfaenglichen Diskussion die sauren Niederschlaege als alleinige Ursache angesehen wurden, zeigte sich spaeter, dass auch Einwirkungen von Spurengasen zur Schaedigung des Waldes beitragen. Dieser Bericht soll auf die Bedeutung der Nebelinterzeption aufmerksam machen, die ebenfalls zum Schadstoffeintrag in den Wald beitraegt. Die Deposition von Wolkenwasser auf einen Waldbestand ist stark abhaengig von der Hoehenlage, in der sich der Waldbestand befindet, so dass auch eine Hoehenabhaengigkeit des Spurenstoffeintrages zu erwarten ist. Durch den Versuch der Quantifizierung traegt dieser Bericht dazu bei, Gebiete zu erkennen, in denen dieser Eintragspfad eine Rolle spielt. (orig./KW)

  9. Potential and possibilities of supplying energy from biomass and biogas; Potentiale und Moeglichkeiten der Energiebereitstellung durch Biomasse und Biogas

    Energy Technology Data Exchange (ETDEWEB)

    Sonnenberg, H. [Bundesforschungsanstalt fuer Landwirtschaft, Braunschweig (Germany). Inst. fuer Betriebstechnik; Weiland, P.; Ahlgrimm, H.J. [Bundesforschungsanstalt fuer Landwirtschaft (FAL), Braunschweig (Germany). Inst. fuer Technologie

    1998-06-01

    Agriculture`s potential contribution to the energy supply of the ``town of the future`` through the conversion of biomass to energy, including biogas production, is a rather modest one. Supposing that the share of total renewable energy in Germany`s primary energy demand rises to approximately 4%, then the proportion of biomass from biotic raw materials especially produced for the purpose will at the most make up an eighth of this amount. Beyond this, biomass is burdened with other drawbacks such as low supply efficiency, limited availability, and weather-dependent reliability. On the other hand, biomass is well suited for conversion to solid, liquid, and gaseous fuels, including inexpensive ones with low energy density (solid fuels), mostly used for stationary heating applications, as well as more expensive ones such as liquid fuels with a high energy density for mobile applications in the automotive sector. Thanks to its capacity to regenerate, biomass is an inexhaustible resource. Moreover, its natural life cycle has a small impact on the environment. [Deutsch] Der Beitrag, den die Landwirtschaft durch energetische Nutzung von Biomasse, z.B. auch mit der Erzeugung von Biogas, zur Energieversorgung der `Stadt der Zukunft` leisten kann, nimmt sich bescheiden aus. Wird erwartet, dass innerhalb des naechsten Jahrzehnts der Anteil regenerativer Energien insgesamt auf etwa 4% des Primaerenergie-Verbrauchs Deutschlands ansteigen koennte, so duerfte Biomasse als speziell zur Energiegewinnung angebaute nachwachsende Rohstoffe mit bestensfalls 0,5 Prozentpunkten daran beteiligt sein. Es beduerfen darueber hinaus auch Nachteile, wie geringe Bereitstellungseffizienz, beschraenkte Verfuegbarkeit und witterungsabhaengige Zuverlaessigkeit, der Beachtung. Die Biomasse kann jedoch mit Erfolg in feste, fluessige und gasfoermige Energietraeger konvertiert werden, sowohl in preiswerte mit geringer Energiedichte (Festbrennstoffe) fuer bevorzugt stationaeren Heizungs-Einsatz als auch

  10. Retrospektive Analyse von Zufallsbefunden, die bei Patienten mit kutanem malignen Malignom durch (18) F-Fluordeoxyglucose-PET/CT erhoben wurden.

    Science.gov (United States)

    Conrad, Franziska; Winkens, Thomas; Kaatz, Martin; Goetze, Steven; Freesmeyer, Martin

    2016-08-01

    Bei der (18) F-Fluordeoxyglucose-Positronenemissionstomographie/Computertomographie (FDG-PET/CT) ergeben sich häufig Zufallsbefunde. In der vorliegenden Studie konzentrierten wir uns auf mittels FDG-PET/CT erhaltene Zufallsbefunde bei Patienten mit kutanem Melanom und überprüften deren Relevanz hinsichtlich weiterer diagnostischer Maßnahmen und Interventionen. Die Krankenakten von 181 konsekutiven Melanom-Patienten wurden retrospektiv ausgewertet, um das Management von Zufallsbefunden zu dokumentieren. Der Schwerpunkt lag dabei auf den histologischen Befunden. Bei 33 von 181 (18 %) Patienten lagen 39 relevante Zufallsbefunde vor, und zwar im Kolorektalbereich (n = 15 Patienten), in der Schilddrüse (n = 8), der Prostata (n = 2), dem Bewegungsapparat (n = 2), in Lymphknoten (n = 2), der Parotis (n = 1), den Mandeln (n = 1), den Nieren (n = 1) und der Gallenblase (n = 1). Bei 25 Patienten schlossen sich weitere diagnostische Verfahren an, wobei in 21 Fällen ein klinisches Korrelat nachgewiesen wurde. Bei 16 von 21 Patienten ergab sich eine Neoplasie, darunter fünf maligne Läsionen (vier Kolonkarzinome und ein Prostatakarzinom). Die Malignome wurden frühzeitig diagnostiziert und in der Mehrzahl der Fälle erfolgreich entfernt. Der Einsatz der FDG-PET/CT als Routine-Diagnostik wird in den Leitlinien empfohlen und ist indiziert bei malignem Melanom ab Stadium IIC. In dieser Studie wurden auf effektive Weise ansonsten nicht erkannte Krebserkrankungen, insbesondere Kolonkarzinome, detektiert. In den meisten Fällen war eine frühe Intervention möglich. Zufallsbefunde durch FDG-PET/CT sollten, unter Berücksichtigung des Zustands und der Wünsche des Patienten, mit den geeigneten diagnostischen Maßnahmen abgeklärt werden. © 2016 Deutsche Dermatologische Gesellschaft (DDG). Published by John Wiley & Sons Ltd.

  11. Hautreizungen durch Propylenglykol

    DEFF Research Database (Denmark)

    Andersen, Klaus Ejner; Storrs, F J

    1982-01-01

    Propylene glycol is used in foods, medicine and cosmetics because it is a good solvent which has, simultaneously, moisture-regulating, antiseptic, and preservative effects. Propylene glycol may produce eczematous skin reactions of toxic and, more rarely, of allergic nature. Positive patch test...

  12. Attenuation corrections through energy spectra analysis of whole body and partial body measurements applying gamma spectroscopy; Schwaechungskorrektur bei gammaspektroskopischen Ganz- und Teilkoerpermessungen durch Analyse der Energiespektren

    Energy Technology Data Exchange (ETDEWEB)

    Schelper, L.F.; Lassmann, M.; Haenscheid, H.; Reiners, C. [Wuerzburg Univ. (Germany). Klinik und Poliklinik fuer Nuklearmedizin

    1997-12-01

    The study was carried out within the framework of activities for testing means of direct determination of radioactivity levels in the human body due to incorporated, inhomogenously distributed radionuclides. A major task was to derive the average depth of activity distributions, particularly from photon radiation at energies below 500 keV, for the purpose of making suitable attenuation corrections. The paper presents two applicable methods which yield information on the mean depths of activity distributions, obtained through additional analyses of the energy spectra. The analyses are based on measuring the dependence of intensity of the Compton radiation on the length of pathways of the photons penetrating the soft tissue, or on measuring the energy-dependent absorption effects with photons. (orig./CB) [Deutsch] Im Rahmen der direkten Aktivitaetsbestimmung bei inhomogener Radionukliddeposition im menschlichen Koerper mittels Ganz- oder Teilkoerpermessanlagen im klinischen Bereich oder im Strahlenschutz sollte besonders bei Photonenstrahlung mit Energien von weniger als 500 keV eine Ermittlung der mittleren Tiefe der Aktivitaetsverteilung zur Schwaechungskorrektur erfolgen. Im klinischen Umfeld ist es haeufig moeglich, zur Tiefenkorrektur die mittlere Organtiefe und damit die schwaechende Gewebsschicht mittels Ultraschall zu bestimmen. Ergaenzend hierzu werden im Folgenden zwei Methoden vorgestellt, welche Aussagen ueber die mittlere Tiefe von Aktivitaetsverteilungen durch Gewinnung von Zusatzinformationen aus dem Energiespektrum im Rahmen von gamma-spektroskopischen Personenmessungen ermoeglichen. Dazu werden einerseits die Abhaengigkeit der Intensitaet der Comptonstrahlung von der Laenge der Wegstrecke von Photonen durch Weichgewebe und andererseits energieabhaengige Absorptionseffekte bei Photonen als Grundlage herangezogen. (orig.)

  13. Enhancing fuel cell system life by removing ammonia. Final report; Erhoehung der Lebensdauer von Brennstoffzellensystemen durch Ammoniak-Entfernung. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-07-01

    Ergebnissen verglichen. So wurden in Laborversuchen Randbedingungen fuer die Ammoniak-Synthese in Reformersystemen in Abhaengigkeit der Parameter N{sub 2}-Konzentration im Erdgas, Temperatur, verwendete Katalysatoren und Raumgeschwindigkeit (Lastabhaengigkeit) aufgezeigt. Ferner wurde auch die Wirkung von Ammoniak auf die einzelnen Katalysatoren eines Dampfreformers untersucht. Zielstellung des Projekts war es ein einfaches, in BZH integrierbares Reinigungsmodul zu entwickeln, welches Ammoniakspuren aus dem Produktgas von Reformierungsprozessen entfernt. Aufgrund der stark hydrophilen Eigenschaft von Ammoniak sollten Verfahren zur Loesung und Abscheiden von Ammoniak in fluessigem Wasser untersucht werden. Dazu wurden unterschiedliche Konzepte technisch realisiert und im Laborversuch auf ihre Tauglichkeit hin untersucht und bewertet. Hier wurden Grenzen der Umsetzbarkeit der Ammoniakentfernung durch Loesen und Abscheiden in fluessigem Wasser aufgezeigt und Moeglichkeiten zur Realisierung einer vollstaendigen Ammoniakentfernung erarbeitet. Mit Hilfe der erzielten Ergebnisse wurde ein optimiertes Labormuster eines Reinigungsmoduls entwickelt, das zum einen eine moeglichst vollstaendige Ammoniak-Reinigung erzielt und zum anderen auch die Produktgaskonditionierung hinsichtlich Temperatur und Feuchte fuer den Brennstoffzelleneinsatz ermoeglicht. Das Reinigungsmodul wurde schliesslich auf einem dafuer modifizierten Teststand mitsamt den dazu benoetigten Peripheriekomponenten vermessen.

  14. Pharmaka mit negativer Auswirkung auf den Knochen – durch Medikamente induzierte Osteoporose // Medications with Negative Effect on Bone – Drug-Induced Osteoporosis

    Directory of Open Access Journals (Sweden)

    Gasser RW

    2016-01-01

    Full Text Available Drug-induced osteoporosis may occur as a side effect of many commonly prescribed drugs. This leads to a disturbance of bone remodelling, which ultimately results in a preponderance of bone loss and – as a consequence – in an increased incidence of bone fractures. Also, a drug-induced disturbance of the calcium metabolism may contribute to bone mineral loss. Glucocorticoids, thyroxine, depot-medroxyprogesterone acetate, aromatase inhibitors, GnRH agonists, thiazolidinediones, proton pump inhibitors, antiepileptic drugs, selective serotonin (and norepinephrine reuptake inhibitors, anticoagulants, loop diuretics, calcineurin inhibitors and antiretroviral active substances can lead to significant bone mineral loss in long-term use. A combination of several bone-toxic substances can potentially amplify the negative effect on bone. When applying the above mentioned drugs over a prolonged period, a possible effect on bone should always be taken into account, especially in older people. An osteological investigation with laboratory and bone densitometry or fracture risk assessment by FRAX is required. For prevention and therapy of drug-induced osteoporosis lifestyle measures, an adequate supply of calcium and vitamin D and optionally a specific osteoporosis treatment with bisphosphonates, denosumab or teriparatide are appropriate. p bKurzfassung/b: Eine durch Medikamente induzierte Osteoporose kann als Nebenwirkung bei vielen häufig verschriebenen Pharmaka auftreten. Dabei kommt es zu einer Störung des Knochenumbaus, wobei letztendlich ein Überwiegen des Knochenabbaus vorliegt. Ein vermehrtes Auftreten von Knochenbrüchen ist die Folge. Auch eine durch Medikamente bedingte Störung des Kalziumstoffwechsels kann zum Knochenmineralverlust beitragen. Glukokortikoide, Thyroxin, Depot-Medroxyprogesteron-Acetat, Aromataseinhibitoren, GnRH-Agonisten, Thiazolidindione, Protonenpumpenhemmer, Antiepileptika, selektive Serotonin- (und Noradrenalin- Reuptake

  15. Properties of polymer-silver nanocomposites fabricated by co-sputtering; Eigenschaften von Polymer-Silber-Nanokompositen hergestellt durch Co-Sputtern

    Energy Technology Data Exchange (ETDEWEB)

    Schuermann, Ulrich

    2006-07-01

    In this thesis polymer-metal nanocomposites were fabricated by simultaneous sputtering of silver and polymers from two independent magnetron sputtering sources. By the netted structure and the dielectric properties PTFE is suited for the use as matrix material. The metal content of the composite films was determined via the measurement of EDX intensities. The size of the nanoparticles was determined by means of TEM and XRD and lies in the range of 10 nm. The composite materials show at the percolation limit a sudden transition of the properties from polymer- to metal-like behaviour. The specific resistance decreases in a range between 35 and 40 % silver content by at least nine orders of magnitude. The optical properties change with the metal content. The absorption caused by plasmon resonance, which has at small filling degrees its maximum at about 000 nm and has an intense yellow colour of the composite material as consequence shifts with increasing silver content to longer wavelengths. The difference in the optical.behaviour of the nanocomposites and the pure polymers can be used for the fabrication of Bragg reflectors. Small changes in the metal content can in the polymer-silver nanocomposites in the range of the percolation threshold effect a drastic change in ther properties, by which applications as sensors are thinkable. [German] In dieser Arbeit wurden Polymer-Metall-Nanokomposite durch gleichzeitiges Sputtern von Silber und Polymeren aus zwei unabhaengigen Magnetron-Sputterquellen hergestellt. Durch die vernetzte Struktur und die dielektrischen Eigenschaften eignet sich PTFE zur Verwendung als Matrixmaterial. Der Metallgehalt der Kompositfilme wurde ueber die Messung von EDX-Intensitaeten ermittelt. Die Groesse der Nanopartikel wurde mit Hilfe von TEM und XRD bestimmt und liegt im Bereich von 10 nm. Die Kompositmaterialien zeigen an der Perkolationsgrenze einen schlagartigen Uebergang der Eigenschaften vom polymer- zum metallaehnlichen Verhalten. Der

  16. Determination of microstructure of high-temperature superconductors by non-resonant microwave-absorption. Final report; Bestimmung der Mikrostruktur von Hochtemperatur-Supraleitern durch nicht-resonante Mikrowellenabsorption. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Dinse, K.P.

    1993-04-01

    Non-resonant microwave absorption has been proven as a highly sensitive method to screen for supraconductivity. Furthermore, in special cases the microstructure of the superconducting material, which is connected by Josephson junctions, can be determined. In single crystals the pinning potential can be measured and it has been demonstrated for the 123 material, that the critical current density is limited by the existence of non-superconducting layers. Decoration of superconducting thin films with stable paramagnetic molecules was utilized to measure the typical length scale of field inhomogenities at the surfaces. These inhomogenities are probably generated by growth inhomogenities characteristic for epitactic thin films. Their influence dominates the field variance expected for an ideal typ II superconductor. (orig.) [Deutsch] Nicht-resonante Mikrowellenabsorption ist ein hochempfindliches Verfahren, um Supraleitung nachzuweisen. Ueber diesen Nachweis hinaus kann in Einzelfaellen die Topologie von supraleitenden Bereichen, die durch Josephsonkontakte getrennt sind, bestimmt werden. In einkristallinem Material ist dann die Bestimmung des Pinningpotentials moeglich und fuer 123-Material konnte gezeigt werden, dass die kritische Stromdichte durch die Existenz nicht-supraleitender Bereiche begrenzt wird. An supraleitenden duennen Filmen ist es durch die Methode der Oberflaechendekoration mit paramagnetischen Spins gelungen, die typische Laengenscala von Feldinhomogenitaeten zu messen, die durch das ``Insel``-Wachstum epitaktischer Filme verursacht wird. (orig.)

  17. Origin and prevention of infection with Legionella pneumophila through cooling towers and evaporative cooling towers; Entstehung und Vermeidung von Legionelleninfektionen durch Kuehltuerme und Rueckkuehlwerke

    Energy Technology Data Exchange (ETDEWEB)

    Schulze-Roebbecke, R. [Bonn Univ. (Germany). Hygiene-Institut; Richter, M. [Staatshochbauamt, Bonn (Germany)

    1994-04-01

    Evaporative cooling towers and industrial ventilator cooling towers have repeatedly been described as the origin of Legionnaires` disease. This article describes the design and function of cooling towers and evaporative cooling towers, sums up knowledge on the colonization of such systems with Legionella pneumophila, and describes conditions permitting the transmission of Legionella. Furthermore, design, maintenance, cleaning and disinfection measures are indicated which are believed to reduce the risk of infection through industrial and evaporative cooling towers. (orig.) [Deutsch] Rueckkuehlwerke klimatechnischer Anlagen und industrielle Ventilator-Kuehltuerme sind mehrfach als Ausgangspunkt von Legionellose-Epidemien beschrieben worden. In der vorliegenden Arbeit werden Aufbau und Funktion von Kuehltuermen und Rueckkuehlwerken erlaeutert, Erkenntnisse ueber die Besiedlung dieser Anlagen mit Legionellen zusammengefassten und die Situationen geschildert, in denen es zur Uebertragung von Legionellen kam. Darueber hinaus werden Konstruktions-, Wartungs-, Reinigungs-, und Desinfektionsmassnahmen aufgefuehrt, die zur Verminderung des Infektionsrisikos durch Kuehltuerme und Rueckkuehlwerke als wirksam angesehen werden. (orig.)

  18. The diesel engine - an economical and environment-friendly drive system thanks to exhaust gas cleaning; Der Dieselmotor - ein gleichzeitig wirtschaftliches und umweltfreundliches Antriebsaggregat durch Abgasreinigung

    Energy Technology Data Exchange (ETDEWEB)

    Huethwohl, G. [HJS Fahrzeugtechnik GmbH und Co., Menden (Germany)

    1998-12-01

    A medium-sized solo bus fleet of some 100 vehicles equipped with EURO II engines emits about 9.5 tonnes of carbon monoxide, 6.5 tonnes of hydrocarbons, 1.3 tonnes of particulates, 1.6 tonnes of sulfur dioxide and 68 tonnes of nitrogen monoxide annually in inner-urban areas. A similar magnitude of emissions can be calculated for other vehicles, for instance inner-urban distribution vehicles or vehicles of the municipal sanitation service. (orig.) [Deutsch] Beim Einsatz moderner Solobusse (EURO II-Motoren) werden durch die Flotte eines Verkehrsbetriebs mittlerer Groesse mit z.B. 100 Fahrzeugen jaehrlich etwa 9,5 t Kohlenmonoxid, 6,5 t Kohlenwasserstoffe, 1,3 t Partikel, 1,6 t Schwefeldioxid und 68 t Stickoxide innerhalb der Innenstadt emittiert. Eine aehnliche Belastung laesst sich fuer andere Fahrzeuge berechnen, wie z.B. innerstaedtische Verteilerfahrzeuge oder kommunale Muell- und Strassenreinigungsfahrzeuge. (orig.)

  19. Reducing available amounts of heavy metals in contaminated soils by adding iron oxides. Final report; Verringerung der Bioverfuegbarkeit von Schwermetallen in kontaminierten Boeden durch Zugabe von Eisenoxiden. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Pluquet, E.; Mueller, I.

    1998-02-01

    This project was part of a German-French research co-operation and investigated the practicability to immobilize heavy metals by adding iron oxides to contaminated soils (harbour sediment, bracky marsh contaminated by emission of a zinc/lead smelter, alluvial Gley soil contaminated by mining activity). Pot and field trials were carried out using three different soils. Five iron bearing materials were added to the soil (1% pure Fe in soil dry matter): Red mud from Al-industry, Fe-precipitation sludge from drinking water treatment, bog iron ore, unused steel shot and steel shot waste from surface treatment of rolled steel plates. When mixed with the soil NH{sub 4}NO{sub 3} and DTPA extractable amounts of heavy metals and uptake by plants was markedly reduced. It was demonstrated that precipitated Fe-bearing sludge from drinking water treatment was a very effective material reducing the NH{sub 4}NO{sub 3} soluble amounts by 40-50% (DTPA 30-40%) in the pot tests while the uptake by plants was reduced by 40% and more. Heavy metals were strongly bound in a fixed order Cd>Pb>Zn>Cu and stayed immobilized until the end of project. Heavy metals were immobilized to a certain extent in field trials, but less effect was observed on concentration in plants and soil extracts compared with the pot tests. In practice the treatment should exceed the 1% pure Fe tested and the application should more homogeneously affect the soil layer where the plant roots mainly grow. (orig.) [Deutsch] Im Rahmen eines deutsch-franzoesischen Projektverbundes wurde die Moeglichkeit der Immobilisierung von Schwermetallen in kontaminierten Boeden durch gezielte Zufuhr von Eisenoxiden untersucht. Die praxisorientierten Untersuchungen wurden in Gefaess- und Feldversuchen mit drei unterschiedlichen Boeden (Spuelfeldboden aus Hafenschlick, immissionsbelastete Brackmarsch, durch Bergbau belasteter Auenboden) ausgefuehrt. Dazu wurden fuenf eisen(oxid)haltige Materialien (Rotschlamm aus der Al

  20. Unterstützung der IT-Service-Management-Prozesse an der Technischen Universität München durch eine Configuration-Management-Database

    Science.gov (United States)

    Knittl, Silvia

    Hochschulprozesse in Lehre und Verwaltung erfordern durch die steigende Integration und IT-Unterstützung ein sogenanntes Business Alignment der IT und damit auch ein professionelleres IT-Service-Management (ITSM). Die IT Infrastructure Library (ITIL) mit ihrer Beschreibung von in der Praxis bewährten Prozessen hat sich zum de-facto Standard im ITSM etabliert. Ein solcher Prozess ist das Konfigurationsmanagement. Es bildet die IT-Infrastruktur als Konfigurationselemente und deren Beziehungen in einem Werkzeug, genannt Configuration Management Database (CMDB), ab und unterstützt so das ITSM. Dieser Bericht beschreibt die Erfahrungen mit der prototypischen Einführung einer CMDB an der Technischen Universität München.

  1. Optimizing biological waste water cleaning by means of modern control systems (fuzzy logic); Optimierung der biologischen Abwasserreinigung durch moderne Regelsysteme (Fuzzy-Logik)

    Energy Technology Data Exchange (ETDEWEB)

    Lohse, M.; Boening, T.; Hegemann, G. [Fachhochschule Muenster (Germany). Inst. fuer Abfall- und Abwasserwirtschaft e.V.

    1999-07-01

    Within the framework of a project sponsored by EUREGIO, test series with the biological activation stages of a German and a Dutch sewage treatment plant each are carried out using different process concepts for the control of oxygen supply by fuzzy logic. As the currently available results demonstrate, the developed fuzzy-logic fields of characteristic curves permit establishing a stable and, thus, little energy-consuming process with optimum oxygen supply in comparison with conventional control. (orig.) [German] Im Rahmen eines von der EUREGIO gefoerderten Forschungsprojektes werden Versuchsreihen im Bereich der biologischen Belebungsstufen einer deutschen und einer niederlaendischen Abwasserreinigungsanlage (ARA) mit unterschiedlichen Verfahrenskonzepten hinsichtlich der Regelung der Sauerstoffzufuhr mit Hilfe der Fuzzy-Logik Technik durchgefuehrt. Die bisherigen Versuchsergebnisse zeigen, dass - im Vergleich zur konventionellen Regelung - durch die entwickelten Fuzzy-Logik Kennfelder ein stabiler und damit energiearmer Prozess mit optimaler Sauerstoffzufuhr erzeugt wird. (orig.)

  2. THERMOSELECT. Continuous environment-friendly treatment of residues by gasification and direct melting; THERMOSELECT. Unterbrechungslose umweltgerechte Restabfallbehandlung durch Vergasung und Direkteinschmelzung

    Energy Technology Data Exchange (ETDEWEB)

    Gaeng, P.; Klein, K.; Stahlberg, R.; Weisenburger, P. [Thermoselect Suedwest GmbH, Karlsruhe (Germany)

    1996-12-31

    The Thermoselect process comprises waste compaction, degassing, gasification with pure oxygen, and melting of inorganic components in a continuous closed cycle process. The high-quality products can be recycled almost completely: The synthesis gas can be used as feedstock or combusted, also in high-efficiency power generation processes. The other products, e.g. the high-grade mineral and metal granulates, can be utilized without restrictions. The residual fraction which must be dumped is very small. (orig) [Deutsch] Mit Thermoselect ist eine neue Technik auf dem Markt, die durch die konsequente Umsetzung der Verfahrensschritte Abfallverdichtung, Entgasung, Vergasung mit reinem Sauerstoff und Einschmelzung der anorganischen Muellbestandteile in einem unterbrechungslosen geschlossenen Prozess hochwertige Produkte aus Abfaellen erzeugt, die nahezu vollstaendig verwertet werden koennen. Das erzeugte Synthesegas kann sowohl stofflich als auch thermisch genutzt werden, wobei der Einsatz von stromerzeugungsverfahren mit hohen Wirkungsgraden moeglich ist. Die ubrigen Produkte, z.B. das mineralische und das metallische Granulat, erreichen eine Qualitaet, die eine uneingeschraenkte Nutzung zulaesst. Die Anteile der z.Zt. noch zu entsorgenden Reststoffe ist gering, wobei derzeit meist wirtschafatliche Gruende deren Entsorgung nahelegen. (orig)

  3. Use of dexpanthenol and aloe vera to influence the irradiation response of the oral mucous membrane (mouse); Beeinflussung der Strahlenreaktion der Mundschleimhaut (Maus) durch Dexpanthenol {+-} Aloe vera

    Energy Technology Data Exchange (ETDEWEB)

    Schlichting, S. [Klinik und Poliklinik fuer Strahlentherapie und Radioonkologie, Dresden Univ. (Germany); Spekl, K.; Doerr, W. [Klinik und Poliklinik fuer Strahlentherapie und Radioonkologie, Dresden Univ. (Germany)]|[Experimentelles Zentrum, Medizinische Fakultaet Carl Gustav Carus, Dresden Univ. (Germany)

    2004-07-01

    In summarising the outcome of the present study it can be said spraying the agent on the oral mucous membrane once a day had an effect on the incidence of mucous membrane ulceration in the case of both placebo and dexpanthenol treatment. However, there was no significant difference between placebo and dexpanthenol treatment, the only finding being a slight prolongation of latency time through aloe vera. These experimental findings give good reason to critically reconsider the clinical use of dexpanthenol as a supportive treatment for the prevention of radiogenic mucositis enoralis following irradiation of tumours in the head and neck region. However thorough oral lavage is an effective means of moderating the irradiation response of the oral mucous membrane. [German] Zusammenfassend ist festzustellen, dass in den vorliegenden Versuchen mit einmal taeglichem Aufspruehen des Praeparates auf die Mundschleimhaut sowohl die Placebo - wie auch die Dexpanthenol-Behandlung die Inzidenz von Schleimhautulzerationen modifiziert hat; zwischen Placebo- und Dexpanthenol-Behandlung ergab sich jedoch kein signifikanter Unterschied. Lediglich eine geringe Verlaengerung der Latenzzeit durch Aloe vera war zu beobachten. Auf der Basis dieser experimentellen Ergebnisse muss der klinische Einsatz von Dexpanthenol im Rahmen der Supportivtherapie zur Prophylaxe der radiogenen Mucositis enoralis bei der Bestrahlung von Kopf-Hals-Tumoren kritisch ueberdacht werden. Regelmaessige, intensive Mundspuelungen sind jedoch ein probates Mittel zur Verminderung der Strahlenreaktion der Mundschleimhaut. (orig.)

  4. Purification of industrial effluents with a high load of organic pollutants by a combined anaerobic/aerobic process; Reinigung organisch hochbelasteter Industriebwaesser durch eine anaerob/aerobe Verfahrenskombination

    Energy Technology Data Exchange (ETDEWEB)

    Reidl, H.H. [AEW ESMIL GmbH fuer Wasser- und Abwassersyteme, Ratingen (Germany)

    1994-12-31

    Organically polluted sewage water can be economically cleaned in an aerobic biological sewage treatment plant with upstream anaerobic as this saves activation volume and ventilation energy. This was demonstrated in the sewage treatment plant of the PWA in Redenfelden, where an anaerobic stage was installed which uses the BIOTHANE{sup R} process. The CARROUSEL-system of the anaerobic stage has proved a istself both in the PWA ans in municipal plants between 5000 to 100000 EW. It is a robust system with uncomplicated control which guarantees observance of maximum values and allows elimation of P and N. (orig.) [Deutsch] Organisch hochbelastete Abwaesser werden oekonomisch durch eine aerobe biologische Klaeranlage mit vorgeschalteter Anaerobie gereinigt, weil dadurch Belebungsvolumen und Belueftungsenergie eingespart werden. Dies wurde am Beispiel der Abwasserreinigungsanlage der PWA Redenfelden gezeigt, bie der eine Anaerobstufe nach den BIOTHANE{sup R}-Verfahren installiert ist. Das dort eingesetzte CARROUSEL-System fuer die Aerobstufe hat sich sowohl bei der PWA als auch im kommunalen Bereich bei Anlagen zwischen 5000 und 100000 EW, als robustes System mit unkomplizierter Steuerung bewaehrt, das eine sichere Einhaltung der Grenzwerte garantiert und die Moeglichkeit zur P- und N-Elimination bietet. (orig.)

  5. Implementation of the provider change process via CRM (Customer Relation Management) system; Umsetzung des Lieferantenwechsels mit CRM-System. Masken fuer Vertrieb und Netz durch System strikt getrennt

    Energy Technology Data Exchange (ETDEWEB)

    Liegl, A. [Stadtwerke Muenchen (Germany). Fachbereich Organisation; Percher, G. [Siemens AG, Graz (Austria)

    2004-03-22

    In October 2001 Stadtwerke Muenchen has set into function the module 'Customer Care' as the first part of a corporate CRM system. The objective oft the project was to gain an overall sight on the customers thus obtaining the necessary basis for customer loyalty programmes and acquisition. The provider change process is up to now marked by a high degree of manual activities. In the future it has to be performed thoroughly and efficiently using CRM functions. Care has to be taken to meet the necessary demands of the provider and the net company with respect to the unbundling requirement. (orig.) [German] Die Stadtwerke Muenchen haben im Oktober 2001 das Modul Kundenkontaktmanagement als erste Stufe eines unternehmensweiten CRM-Systems in Betrieb genommen. Ziel des Projekts war es, eine ganzheitliche Kundensicht zu ermoeglichen und so die Voraussetzungen fuer Kundenbindung und Kundengewinnung zu schaffen. Der bisher durch manuellen Aufwand gepraegte Prozess des Lieferantenwechsels soll nun mit CRM-Funktionen durchgaengig und effizient abgewickelt werden. Dabei wird den Beduerfnissen sowohl des Netzbetreibers als auch des Vertriebs unter Beachtung der Vorschriften des Unbundlings Rechnung getragen. (orig.)

  6. Bubble-free ozone addition through ceramic membranes for wet-oxidative waste water treatment; Blasenfreier Ozoneintrag durch keramische Membranen zur nassoxidativen Abwasserbehandlung

    Energy Technology Data Exchange (ETDEWEB)

    Janknecht, P.; Wilderer, P.A. [Technische Univ. Muenchen, Garching (Germany). Lehrstuhl und Pruefamt fuer Wasserguete- und Abfallwirtschaft

    1999-07-01

    A prerequisite for successful wet oxidation is very accurately tuned and carefully monitored process control. In the alternative, a decline in water quality is actually possible. In particular, earlier studies in the ozonification of landfill leachate encountered problems in reducing levels of AOX in the presence of halogenated hydrocarbons. Serious problems in process control may arise when ozone is conventionally added and forms bubbles in the presence of surface-active substances; this foam accumulates and is so persistent as to evade mechanical control. Since the formation of foam is directly due to gas bubbles carried in, bubble-free addition of ozone through a membrane may be a viable approach. (orig.) [German] Voraussetzung fuer den Erfolg einer Nassoxidation ist eine sehr genau eingestellte und sorgfaeltig ueberwachte Prozessfuehrung, da anderenfalls auch eine Verschlechterung der Wasserqualitaet eintreten kann; insbesondere haben sich hier bei frueheren Untersuchungen zur Ozonung von Deponiesickerwaessern Schwierigkeiten bei der Reduzierung des AOX-Wertes in Anwesenheit von halogenierten Kohlenwasserstoffen ergeben. Gravierende Schwierigkeiten in der Prozessfuehrung kann Schaum bereiten, der sich bei konventionellem Blaseneintrag des Ozons in Anwesenheit von oberflaechenaktiven Substanzen bildet, sich in der Anlage ansammelt und dabei so bestaendig ist, dass er auf mechanische Weise nicht zu kontrollieren ist. Da die Schaumbildung direkt auf die eingetragenen Gasblasen zurueckzufuehren ist, stellt der blasenfreie Eintrag von Ozon durch eine Membran einen moeglichen Loesungsansatz dar. (orig.)

  7. Control-technical optimization of topped denitrification through fuzzy control; Regelungstechnische Optimierung der vorgeschalteten Denitrifikation durch Anwendung von Fuzzy-Control

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, U. [Hydroplan Ingenieurgesellschaft, Worms (Germany); Poepel, H.J. [Technische Univ. Darmstadt (Germany). Inst. WAR - Wasserversorgung, Abwassertechnik, Abfalltechnik, Umwelt- und Raumplanung

    1999-07-01

    The present paper describes complex fuzzy systems for controlling nitrogen elimination at plants with topped denitrification. For the design and testing of the fuzzy systems, dynamic simulation calculations and experimental tests were carried out in a semi-technical pilot plant. The controlling fuzzy systems indicate the supposed oxygen values for individual tank areas and the most appropriate partitioning of the activated sludge tank (anoxic/aerobic zone) as a function of the input quantities used. It is established that, with the more flexible control behaviour, a more stable nitrogen elimination and, at the same time, a cut in the amount of air transferred to the system can be attained in comparison with a conventional control. (orig.) [German] Im Rahmen dieses Beitrags werden komplexe Fuzzy-Systeme zur Regelung der Stickstoffelimination in Anlagen mit vorgeschalteter Denitrifikation vorgestellt. Zum Entwurf und zur Erprobung der Fuzzy-Systeme wurden dynamische Simulationsrechnungen und experimentelle Untersuchungen an einer halbtechnischen Versuchsanlage durchgefuehrt. Die uebergeordneten Fuzzy-Systeme geben in Abhaengigkeit der verwendeten Eingangsgroessen Sauerstoffsollwerte fuer die einzelnen Beckenbereiche und die jeweils guenstigste Aufteilung des Belebungsbeckens (anoxische/aerobe Zone) vor. Die Ergebnisse zeigen, dass durch das flexible Reglerverhalten eine stabilere Stickstoffelimination und gleichzeitig eine Einsparung an eingetragener Luftmenge im Vergleich zu einem konventionellen Regelsystem erreicht werden kann. (orig.)

  8. Substitution of wastes for fuels and raw materials in high-temperature processes; Substitution von Brennstoffen und Rohstoffen durch Abfaelle in Hochtemperaturprozessen

    Energy Technology Data Exchange (ETDEWEB)

    Scholz, R. [Technische Univ. Clausthal, Clausthal-Zellerfeld (Germany). Inst. fuer Energieverfahrenstechnik; Beckmann, M. [Clausthaler Umwelttechnik-Institut GmbH (CUTEC), Clausthal-Zellerfeld (Germany)

    1998-09-01

    burning processes in the cement industry. To do justice to the steel and iron industry it also deals with fuel substitution in a natural gas fired cokeless cupola furnace. [Deutsch] Die energetische und die stoffliche Verwertung von Abfaellen ist ein bereits seit langem diskutiertes Thema. Dabei interessieren zunaechst die Fragestellungen im Zusammenhang mit dem jeweils spezifischen Einsatz wie z.B. bei der Co-Verbrennung von Klaerschlamm in Kraftwerksfeuerungen, der Substitution von Primaerbrennstoffen durch Kunststoffabfaelle beim Brennprozess in der Zementindustrie usw. Darueber hinaus wird weiter der Vergleich der jeweiligen Anwendung untereinander sowie die Gegenueberstellung mit den zum Stand der Technik gehoerenden thermischen Abfallbehandlungsverfahren diskutiert. Diese Verfahrensvergleiche muessen selbstverstaendlich unter Einbeziehung aller insgesamt erforderlichen Aufwendungen an Zusatzstoffen und Zusatzenergie mit gleichen Randbedingungen durchgefuehrt werden. Dabei sind u.a. die sich aus den spezifischen Eigenschaften der Abfallstoffe fuer den jeweiligen Anwendungsfall ergebenden Besonderheiten zu beruecksichtigen. Das betrifft insbesondere die Auswirkungen einer Substitution von Primaerbrennstoffen durch Ersatzbrennstoffe aus Abfaellen (Sekundaerbrennstoffe) z.B. bei einem Brennprozess auf die Waermeuebertragungsbedingungen, die Stroemungsverhaeltnisse und damit zusammenhaengend auf die Temperaturverteilungen, den Guttransport und den spezifischen Energieaufwand. Ersatzbrennstoffe muessen sich sowohl aus stofflicher Sicht als auch aus brennstoff- und waermetechnischer Sicht fuer eine Substitution eignen. In dem vorliegenden Beitrag wird insbesondere auf die Anforderungen an Abfaellen, die zur Substitution von Primaerbrennstoffen dienen aus brennstoff- und waermetechnischer Sicht eingegangen. Hierzu werden zunaechst kurz einige wesentliche Gesichtspunkte der Waermeuebertragung in Feuerungen und Industrieoefen eroertert. Wichtig fuer die Bewertung einer

  9. Drag-out of stainless-steel alloying constituents through condensates which collect in high-efficiency boilers; Austrag von Legierungsbestandteilen aus nichtrostenden Staehlen durch Kondensate in Brennwertanlagen

    Energy Technology Data Exchange (ETDEWEB)

    Arlt, N. [Thyssen Stahl AG, Werk Krefeld (Germany). Werkstofftechnik Rostfrei; Schlerkmann, H. [Mannesmannroehren-Werke AG, Duisburg (Germany). Korrosionschemie; Stellfeld, I. [Krupp Hoesch Stahl AG, Werksgruppe Duesseldorf (Germany). Metallurgische Technologie; Steinbeck, G. [Verein Deutscher Eisenhuettenleute (VDEh), Duesseldorf (Germany). Fachbereich Werkstofftechnik

    1994-11-07

    Heating systems which are equipped with high- efficiency boilers save energy because the energy content of the flue gases is utilized through flue gas condensation. Stainless steel is an important high-efficiency boiler material. The chromium and nickel concentrations of acid condensates from high- efficiency systems in waste water were analyzed. Stainless-steel samples were immersed in a model condensate over a period of 10 weeks. Fresh model condensates were prepared after one week and three weeks to measure metal dissolution as a function of time. The longer the samples were immersed the more did the nickel concentrations in the model concentrate decrease. Without exception, the concentrations were clearly below the maximum permissible values laid down in leaflet M 251 which is issued by Abwassertechnische Vereinigung e.V. The expected inadmissibly high nickel concentrations did not occur in the acid condensates. (orig.) [Deutsch] Heizungsanlagen mit Brennwertkesseln arbeiten energiesparend, da durch eine Abgaskondensation der Energieinhalt der Abgase nutzbar gemacht wird. Ein wichtiger Konstruktionswerkstoff fuer Brennwertanlagen ist nichtrostender Stahl. Untersucht werden sollte die Frage, ob die sauren Kondensate der Brennwertanlagen, die ins Abwasser abgefuehrt werden unzulaessig hohe Chrom- und Nickelkonzentrationen aufweisen koennen. Zu diesem Zweck wurden Proben aus nichtrostenden Staehlen ueber einen Gesamtzeitraum von 10 Wochen in einem Modellkondensat ausgelagert. Dabei wurde das Modellkondensat nach einer Woche und nach drei Wochen Auslagerungzeit frisch angesetzt, um die zeitliche Abhaengigkeit der Metallaufloesung zu erfassen. Es wurde festgestellt, dass die Nickelkonzentrationen im Modellkondensat mit zunehmender Auslagerungsdauer drastisch abnehmen; sie lagen in allen Faellen deutlich unter den zulaessigen Richtwerten des Merkblattes M 251 der Abwassertechnischen Vereinigung e.V. Die Vermutung, dass unzulaessig hohe Konzentrationen an Nickel in

  10. Genes and Gene Therapy

    Science.gov (United States)

    ... correctly, a child can have a genetic disorder. Gene therapy is an experimental technique that uses genes to ... or prevent disease. The most common form of gene therapy involves inserting a normal gene to replace an ...

  11. Study on the planning of a demonstration plant for hydrogen fuel production by electrolysis using caching in salt caverns under pressure; Studie ueber die Planung einer Demonstrationsanlage zur Wasserstoff-Kraftstoffgewinnung durch Elektrolyse mit Zwischenspeicherung in Salzkavernen unter Druck

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-02-05

    In a two year research project the long-term storage of hydrogen as energy carrier has been assessed in detail for large renewable electricity quantities in underground storage caverns in Germany. Next to a regions analysis for potential storage sites, the benchmarking against other large scale storage concepts, a comparative and perspective analysis of alkaline and PEM-electrolysers also potential business cases for Power-to-Hydrogen in the mobility sector as well as for the chemical, electricity and natural gas industry have been analysed. [German] Durch den geplanten Ausbau der Stromerzeugung aus fluktuierenden, erneuerbaren Energien in Deutschland ruecken Speichertechnologien fuer elektrische Energie immer staerker in den Fokus des industriellen und politischen Interesses. Eine vielversprechende Moeglichkeit fuer eine Langzeitspeicherung bei hohen Anteilen von Wind- und Photovoltaikanlagen sind Speichertechnologien wie die Wasserstoffspeicherung mit Hilfe von Wasserelektrolyseuren. Diese koennen erneuerbaren Strom in grossen Mengen und bei entsprechender Steuerung selektiv in Zeiten mit hohem Dargebot z.B. an Windstrom chemisch speichern. Der gespeicherte Wasserstoff kann dann entweder zu einem spaeteren Zeitpunkt wieder rueckverstromt oder direkt stofflich verwertet werden, z.B. als Kraftstoff fuer den Verkehrssektor, als chemischer Rohstoff oder fuer den Hausenergiebereich durch Einspeisung in das Erdgastransportnetz. Thema der vorliegenden Studie sind Analysen und Planungen fuer die Erprobung des Gesamtsystems ''Wasserstoff-Elektrolyse-Speicherung'' in energiewirtschaftlich relevanten Dimensionen. Dazu werden mit einem neuen Ansatz techno-oekonomische Entwicklungspfade fuer Wasserstoff-Systeme unterschiedlicher Groesse und Technologien charakterisiert und technologische Risiken bei der Realisierung dieser Systeme identifiziert und bewertet. Diese Arbeiten werden ergaenzt durch Arbeiten auf dem Gebiet der Salzkavernenspeicherung in Form

  12. Regulatorisch-toxikologische Maßnahmen zur Minimierung von Verbraucherrisiken in Deutschland und Europa am Beispiel von Lungenschäden durch dünnflüssige Lampenöle auf Petroleumdestillat- und Paraffinbasis

    OpenAIRE

    Hahn, Axel

    2014-01-01

    Vergiftungen mit flüssigen Kohlenwasserstoffen beim Menschen sind seit Anfang des letzten Jahrhunderts bekannt. In den 1950er Jahren waren diese Vergiftungen die Hauptursache für Klinikaufnahmen bei Vergiftungen mit Haushaltschemikalien im Kindesalter in den USA. Erste Hinweise auf eine Gefährdung durch dünnflüssige Lampenöle auf Petroleumdestillat- und Paraffinbasis, die Brennstoffe in Öl- oder Zierlampen waren, kamen aus Skandinavien. Hinweise auf ein besonderes Risiko für Kleinkinder ergab...

  13. Climate protection by reducing the emissions of greenhouse gases in households and the tertiary sector through climate-conscious behaviour. Vol. 2; Klimaschutz durch Minderung von Treibhausgasemissionen im Bereich Haushalte und Kleinverbrauch durch klimagerechtes Verhalten. Bd. 2. Gewerbe, Handel und Dienstleistung

    Energy Technology Data Exchange (ETDEWEB)

    Boede, U.; Gruber, E. [Fraunhofer-Institut fuer Systemtechnik und Innovationsforschung (ISI), Karlsruhe (Germany); Deutscher, P.; Elsberger, M.; Rouvel, L. [Technische Univ., Muenchen (Germany). Lehrstuhl fuer Energiewirtschaft und Innovationsforschung; Brakhage, A.; Jaekel, M. [Abacus, Trier (Germany); Renner, G. [Innovative Energieberatung, Koeln (Germany); Brohmann, B.; Cames, M.; Herold, A.

    2000-08-01

    The aim of the project was to identify areas in households and the tertiary sector in which changes in behaviour could result in energy conservation and thus a reduction of greenhouse gas emissions, and to quantify the potentials for 1995, 2005 and 2020. A second focus was on the analysis and evaluation of programmes and instruments to realise the potentials. With literature evaluation, expert interviews, and a household servey potentials and further technical development have been identified. In sum, behavioural measures can contribute to the CO2 reduction by 64 million tons in 1995 in households and 27 in the commercial sector in which the potential decreases to 18 million tons in 2020 due to the autonomous technical development. Adequate promotion programmes can help to realise 20-30% of the potential by 2020. (orig.) [German] Ziel des Vorhabens war, im Sektor private Haushalte und Kleinverbrauch Bereiche zu identifizieren, in denen Verhaltensaenderungen zur Energieeinsparung fuehren koennen, und diese Potenziale fuer 1995, 2005 und 2020 zu quantifizieren. Darauf aufbauend waren Programme und Instrumente zur Umsetzung aufzuzeigen und zu bewerten. Gestuetzt auf Literaturrecherchen und Expertengespraeche wurden Einzelpotenziale, Rahmenbedingungen, Entwicklungstrends in der Technik und im Ausstattungsgrad ermittelt. Insgesamt koennten Verhaltensmassnahmen im Haushaltssektor die CO2-Emissionen im Basisjahr 1995 um 64 Mio, im Kleinverbrauch um 27 Mio t vermindern. Bis 2020 bleibt dieses Potenzial im Haushaltssektor in etwa gleich. Im Kleinverbrauch sinkt es infolge der autonomen Technikentwicklung auf 18 Mio t ab. Durch geeignete Programme koennen bis 2020 etwa 20-30% des Potenzials erreicht werden. (orig.)

  14. Instruments. Climate protection of reduction of no-load losses in electric appliances and equipment; Klimaschutz durch Minderung von Leerlaufverlusten bei Elektrogeraeten. Instrumente

    Energy Technology Data Exchange (ETDEWEB)

    Rath, U.; Hellmann, R. [Ingenieurbuero fuer Energieberatung, Haustechnik und Oekologische Konzepte GbR (eboek), Tuebingen (Germany); Moehring-Hueser, W.; Wortmann, K.; Bregas, J. [Energiestiftung Schleswig-Holstein, Kiel (Germany); Mordziol, C. [Umweltbundesamt, Berlin (Germany)

    1999-12-01

    Two studies on the subject of 'Climate Protection through Reduction of No-load Losses in Electric Applicances and Equipment' have been performed on behalf of the German Federal Ministry for the Environment, Nature Conservation and Nuclear Safety and the German Federal Environmental Agency. The first study, carried out by the Tuebingen-based engineering bureau eboek, has been published in the Federal Environmental Agency's TEXTE series (Texte 45/97, 2{sup nd} edition, 1998). It was the starting point for a multitude of activities among them two information campaigns carried out in 1998 and 1999 that were funded by the Federal Ministry for the Environment and the German Section of the Friends of the Earth (BUND). The study also served as a basis for decisions taken by the German Bundestag and the Bundesrat (Council of Constituent States). The second study on the subject was carried out by the engineering bureau eboek and Energiestiftung Schleswig-Holstein. Building on the results obtained in the first study, it presents estimates of relevant data for the European Union and examines various approaches for possible measures to reduce no-load losses as to their suitability. The studies impressively show that effective climate protection can be achieved in all relevant sectors, and in many cases even save costs. The results are detailed below. (orig.) [German] Im Auftrag des Bundesministeriums fuer Umwelt, Naturschutz und Reaktorsicherheit sowie des Umweltbundesamtes wurden zwei Studien zum Thema: 'Klimaschutz durch Minderung von Leerlaufverlusten bei Elektrogeraeten' durchgefuehrt: Die erste, vom Ingenieurbuero fuer Energieberatung, Haustechnik und oekologische Konzepte (eboek), Tuebingen, earbeitete Studie wurde in der Reihe TEXTE des UBA veroeffentlicht (Texte 45/97, 2. Auflage 1998). Sie bildete den Ausgangspunkt fuer eine Vielzahl von Aktivitaeten, unter anderem zwei vom Bundesumweltministerium finanzierte und vom Bund fuer Umwelt und

  15. Verminderung des Personalbedarfs durch den Einsatz einer computerbasierten Histologieprüfung [Reduction of the need for personel with a computer-based histology examination

    Directory of Open Access Journals (Sweden)

    Woermann, Ulrich

    2006-08-01

    Histologieprüfung insgesamt benötigten Personentage von 21 auf 6 zu reduzieren. Der materielle Aufwand ist mit 2 Prüfungs-PCs und einem Server-PC bescheiden. Sicherheitsprobleme bestehen bei einem in sich geschlossenen lokalen Netzwerk nicht. Durch kontinuierliche Speicherung der Prüfungsdaten auf dem Server wird ein Datenverlust vorgebeugt. Psychometrisch war die computerbasierte Histologieprüfung gleichwertig zur früher benutzten mündlich-strukturierten Prüfung. Der Einsatz von Computern kann den hohen Ressourcenbedarf von OSCE bzw. OSPE vermindern. Er kann somit mithelfen, dass ein wertvolles Prüfungsverfahren nicht an der beschränkten Verfügbarkeit von Personl scheitert.

  16. Progressive management of distribution systems by automatic meter reading. Research project shows potentialities for cost savings in water distribution in rural areas; Modernes Netzmanagement mit Zaehlerfernablesung. Forschungsprojekt zeigt deutliche Einsparpotenziale durch Fernablesung fuer die Wasserversorgung im laendlichen Raum

    Energy Technology Data Exchange (ETDEWEB)

    Koewener, D. [Fraunhofer-Inst. fuer Systemtechnik und Innovationsforschung (Fraunhofer ISI), Karlsruhe (Germany); Fuhrmann, T. [Witten/Herdecke Univ. (Germany). Inst. fuer Umweltechnik und Management

    2003-07-01

    Network monitoring of extensive water distribution systems as you can find in rural areas causes high costs. Therefore a current research project tries to find cost savings by using automatic meter reading of main flow meters within the network. The project focuses on improving leakage control as well as optimizing rehabilitation investments. By operation of a pilot plant significant potentialities for cost savings already could be identified. Additionally the calculation of the optimal time of rehabilitation shows that automatic meter reading is able to reduce investment costs. For the calculation of the costs the research team developed a software that supports the user in this decision making process. (orig.) [German] In grossraeumigen Wasserversorgungsnetzen, wie sie vor allem im laendlichen Raum anzutreffen sind, ist die Netzueberwachung mit erheblichem Aufwand verbunden. In einem laufenden Forschungsvorhaben wird untersucht, inwieweit sich Einsparungen durch Einsatz von fernablesbaren Grosswasserzaehlern realisieren lassen. Im Zentrum steht dabei die Verbesserung der Leckageueberwachung sowie eine Optimierung der Reinvestitionsplanung. Anhand ausgewaehlter Untersuchungsgebiete des beteiligten Wasserversorgers konnten bereits eindeutige Einsparpotenziale im Netz- und Leckagenmanagement durch den Einsatz der Fernablesung nachgewiesen werden. Auch die Berechnung des betriebswirtschaftlich optimalen Reinvestitionszeitpunkts laesst darauf schliessen, dass der Einsatz der Fernablesung zu einer Kostenreduktion fuehrt. Zur Berechnung der Kosten wurde eine Software entwickelt, die den Anwender bei seiner Entscheidungsfindung unterstuetzt. (orig.)

  17. Low-cost development of the Algyoefield in Hungary by means of horizontal re-entry boreholes; Die kostenguenstige Entwicklung des Algyoe-West-Feldes in Ungarn durch horizontale Re-Entry-Bohrungen

    Energy Technology Data Exchange (ETDEWEB)

    Kinzel, H. [Weatherford Oil Tool GmbH, Langenhagen (Germany); Osz, A. [Magyar Olaj (MOL), Budapest (Hungary); Kerk, T. [Becfield Drilling Services, Edemissen-Berkhoepen (Germany)

    1998-12-31

    Planning and implementation of a typical re-entry borehole in southern Hungary are described. The cost is about half that of a new borehole, and the available infrastructure can be used. The production index of a typical Algyoehorizontal borehole is higher by a factor of 12 than for a vertical borehole in the same field. (orig.) [Deutsch] Anhand einer Beispielbohrung (Algyoe488) wird die Planung und Durchfuehrung einer typischen Re-Entry Bohrung in Suedungarn beschrieben. Durch die weitgehende Verwendung von standardisierten Komponenten und Verfahren sowie durch die enge Zusammenarbeit zweier deutscher Service Unternehmen mit dem Auftraggeber wurde bei insgesamt 35 Horizontalbohrungen in Ungarn der Effekt der Lernkurve zur optimierten Erstellung der Bohrung und damit zur Kostensenkung effektiv eingesetzt. Die so aufgearbeiteten Bohrungen werden im Vergleich zu einer neuen Bohrung fuer etwa die Haelfte der Kosten erstellt. Die vorhandene Infrastruktur des Feldes kann weiter verwendet werden. Der Produktionsindex einer typischen AlgyoeHorizontalbohrung liegt um den Faktor 12 hoeher als eine Vertikalbohrung im gleichen Feld. (orig.)

  18. Measurements of size distributions of radon progeny for improved quantification of the lung cancer risk emanating from exposure to radon decay products; Messungen der Groessenverteilungen von Radon-Folgeprodukten zur Verbesserung der Quantifizierung des durch Radonexposition verursachten Lungenkrebsrisikos

    Energy Technology Data Exchange (ETDEWEB)

    Haninger, T.

    1997-12-31

    A major issue in radiation protection is to protect the population from the harmful effects of exposure to radon and radon progeny. Quantification of the lung cancer risk emanating from exposure to radon decay products in residential and working environments poses problems, as epidemiologic studies yield information deviating from the results obtained by the indirect method of assessment based on dosimetric respiratory tract models. One important task of the publication here was to characterize the various exposure conditions and to quantify uncertainties that may result from application of the ``dose conversion convention``. A special aerosol spectrometer was therefore designed and built in order to measure the size distributions of the short-lived radon decay products in the range between 0.5 nm and 10 000 nm. The aerosol spectrometer consists of a three-step diffusion battery with wire nets, an 11-step BERNER impactor, and a detector system with twelve large-surface proportional detectors. From the measured size distributions, dose conversion coefficients, E/P{sup eq}, were calculated using the PC software RADEP; the RADEP program was developed by BIRCHALL and JAMES and is based on the respiratory tract model of the ICRP. The E/P{sup eq} coefficients indicate the effective dose E per unit exposure P{sup eq} to radon decay products. (orig./CB) [Deutsch] Eines der groessten Probleme des Strahlenschutzes ist der Schutz der Bevoelkerung vor einer Strahlenexposition durch Radon und seine Folgeprodukte. Die Quantifizierung des Lungenkrebsrisikos, das durch Radonexpositionen in Wohnungen und an Arbeitsplaetzen verursacht wird, ist ein grosses Problem, weil epidemiologische Studien ein anderes Ergebnis liefern, als die indirekte Methode der Abschaetzung mit dosimetrischen Atemtrakt-Modellen. Eine wichtige Aufgabe der vorliegenden Arbeit war es, unterschiedliche Expositionsbedingungen zu charakterisieren und die Unsicherheiten zu quantifizieren, die sich aus der

  19. Remediation of heavy-metal contaminated sediments by means of bioleaching. Study: project results - economic efficiency - market assessment. Final report; Reinigung schwermetallbelasteter Sedimente durch Bioleaching. Studie: Projektergebnisse - Wirtschaftlichkeit - Marktbewertung. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Loeser, C.; Zehnsdorf, A.; Hoffmann, P.; Seidel, H.; Schmerold, R.

    2002-07-01

    Sedimente mit Sauerstoff in Kontakt, gehen die Schwermetalle durch mikrobielle Oxidations- und Versauerungsprozesse teilweise in Loesung. Dieses natuerliche Bioleaching, welches unkontrolliert ein Gefahrenpotential fuer die Umwelt darstellt, soll durch Aktivierung der autochthonen schwefeloxidierenden Bakterien (z.B. Thiobacilli) beschleunigt und zur Reinigung der Sedimente genutzt werden. Ziel ist es, ein naturnahes Verfahren zur Sedimentdekontamination unter Nutzung des natuerlichen biologischen Solubilisierungspotentials bereitzustellen. Die gereinigten Sedimente sollen in den Stoffkreislauf als Bodensubstrat zurueckgefuehrt werden. Die Verfahrensentwicklung erfolgte am Beispiel von hochbelasteten Sedimenten der Weissen Elster aus dem Suedraum Leipzig. Aus oekonomischen Gruenden soll das Bioleaching der Schwermetalle durch Festbettlaugung nach dem Perkolationsprinzip erfolgen. Das Verfahren erfordert mehrere Prozessstufen. Der Schwerpunkt der Untersuchungen im Labor- und Technikumsmassstab lag auf der Erarbeitung und technischen Umsetzung von Massnahmen zur Beschleunigung der Metallsolubilisierung. (orig.)

  20. Racism, Ethnicity and the Media in Africa: Reflections Inspired by Studies of Xenophobia in Cameroon and South Africa Rassismus, Ethnizität und die Medien in Afrika: Reflektionen angeregt durch Studien zu Fremdenfeindlichkeit in Kamerun und Südafrika

    Directory of Open Access Journals (Sweden)

    Francis B. Nyamnjoh

    2010-01-01

    Full Text Available This paper demonstrates the extent to which the media and belonging in Africa are torn between competing and often conflicting claims of bounded and flexible ideas of culture and identity. It draws on studies of xenophobia in Cameroon and South Africa, inspired by the resilience of the politicization of culture and identity, to discuss the hierarchies and inequalities that underpin political, economic and social citizenship in Africa and the world over, and the role of the media in the production, enforcement and contestation of these hierarchies and inequalities. In any country with liberal democratic aspirations or pretensions, the media are expected to promote national citizenship and its emphasis on large-scale, assimilationist and territorially bounded belonging, while turning a blind eye to those who fall through the cracks as a result of racism and/or ethnicity. Little wonder that such an exclusionary articulation of citizenship is facing formidable challenges from its inherent contradictions and closures, and from an upsurge in the politics of recognition and representation by small-scale communities claiming autochthony at a historical juncture where the rhetoric espouses flexible mobility, postmodern flux and discontinuity. Der vorliegende Beitrag zeigt auf, inwieweit die Medien und gesellschaftliche Bindungen in Afrika zwischen konfligierenden Ansprüchen abgegrenzter und sich wandelnder kultureller Identitäten zerrissen sind. Angeregt durch die Erfahrung der kontinuierlichen Politisierung kultureller und sozialer Identitäten zieht der Autor Studien zu Fremdenfeindlichkeit in Kamerun und Südafrika heran, um die Hierarchien und Ungleichheiten zu diskutieren, auf denen politische, wirtschaftliche und soziale Staatsbürgerschaft in Afrika und darüber hinaus basiert, sowie die Rolle der Medien bei der Entstehung, Verstärkung und im Wettstreit dieser Hierarchien und Ungleichheiten. In jedem liberal-demokratisch ausgerichteten Staat

  1. Energy conservation in refrigerating machines by combining of thermo-static expansion valve and interior heat transfer material; Energieeinsparung in Kaelteanlagen durch Kombination von thermostatischem Expansionsventil und innerem Waermeuebertrager

    Energy Technology Data Exchange (ETDEWEB)

    Tambovtsev, Alexander

    2007-12-15

    bekannten Komponenten TEV und IWUeals inkompatibel miteinander, weil die einfache Aneinanderreihung zu einem instabilen Regelverhalten fuehrt. Nach detaillierter Analyse der Ursachen dieser Instabilitaet wird die Hypothese aufgestellt, dass durch eine absichtliche ''Verschlechterung'' des IWUeein stabiler Arbeitsbereich gefunden werden kann. Bei dieser ''Verschlechterung'' kann es sich z. B. um den Bypass eines Teils des Hochdruckstromes oder um eine Gleichstromanordnung der Stroeme im Waermeuebertrager handeln. Diese Hypothese wurde primaer durch Experimente, aber zusaetzlich auch durch Simulationsrechnungen bestaetigt. Dafuer wurde ein Versuchsstand aufgebaut, mit welchem verschiedene Konfigurationen und Regelstrategien getestet wurden. Es wurde eine deutliche Verringerung des Energieverbrauchs gegenueber herkoemmlichen Anordnungen erreicht. (orig.)

  2. Mathematical modelling of transport of gaseous and liquid substances induced by pyrite oxidation in spoil banks of lignite mines; Mathematische Modellierung der durch Pyritoxidation induzierten Stofftransporte in Braunkohleabraumkippen in gasfoermiger und fluessiger Phase

    Energy Technology Data Exchange (ETDEWEB)

    Ludwig, R.

    2001-07-01

    Pyrite (FeS{sub 2}) oxidation due to open-pit lignite mining activities may result in acid mine drainage and dissolved metals in the soil and ground water of the overburden spoil piles. The pyrite oxidation takes place firstly during the mining process and at the temporary surfaces of the mine, secondly in deep zones of the overburden spoil pile as a result of gas transport through the unsaturated zone. The second process continues after mine closure possibly over several decades and therefore contaminates the environment for a long time. Whereas some studies deal with a minimisation by geochemical means, this research focuses on a minimisation by impacting the physical boundary conditions. Special attention is given to the transport of oxygen from the soil surface to the pyrite oxidation zone. Soil column experiments with columns of 2.90 m height yield experimental data concerning the pyrite oxidation and the related transport processes in the unsaturated zone. Experimental data are compared to simulation results obtained with the model MUSIC; it calculates coupled reaction and transport processes in a one-dimensional soil profile using the finite volume method. Pyrite oxidation is described by means of a first order kinetics, the gas transport takes place by diffusion as well as convection. The transport of solutes in the soil water is described by the convection-dispersion equation (Cl{sup -}, Fe{sup 2+} and SO{sub 4}{sup 2-}). The simulations are able to reproduce the measured time series of oxygen concentrations in the soil profile. (orig.) [German] Die durch Braunkohlegewinnung im Tagebau hervorgerufene Pyritoxidation (Pyrit: FeS{sub 2}) kann zu Versauerung, Schwermetallfreisetzung und Aufmineralisierung des Boden- und Grundwassers in den Abraumkippen fuehren. Die Pyritoxidation findet zum einen waehrend des Abbaubetriebs und an den temporaeren Oberflaechen des Tagebaus statt, zum anderen in tieferen Bereichen der Abraumkippe durch Gastransport durch die

  3. Effects of SO{sub 2}-fumigation on the infection of Norway spruce by Armillaria ostoyae; Einfluss von SO{sub 2} auf die Infektion von Fichtensaemlingen durch Armillaria ostoyae

    Energy Technology Data Exchange (ETDEWEB)

    Horak, M. [Tuebingen Univ., Spezielle Botanik 1 (Germany); Tesche, M. [TU Dresden, Inst. fuer Forstbotanik und Forstzoologie, Tharandt (Germany)

    1993-04-01

    SO{sub 2} fumigation (0.86 mg/m{sup 3}) reduced drastically the survival expectancy of spruce seedlings already after 1 month. Under such conditions, the infection of spruce by Armillaria ostoyae is known to increase. The present investigation has shown that mortality increased strongly by combining A. ostoyae infection and fumigation. However, mycorrhization of spruce (Paxillus involutus) increased the survival rate of seedlings and reduced infection by A. ostoyae remarkably (20%). (orig.) [Deutsch] Eine SO{sub 2}-Behandlung von 0,86 mg/m{sup 3} fuehrte bereits nach 1 Monat zu einer verminderten Ueberlebensrate der untersuchten Fichtensaemlinge. Unter diesen Bedingungen lag eine erhoehte Infektionsbereitschaft der Fichten gegenueber dem Schwaecheparasiten Armillaria ostoyae vor. Durch eine Mykorrhizierung der Saemlinge konnte die Infektion mit A. ostoyae in starkem Masse (um 20%) eingeschraenkt werden. (orig.)

  4. Superconductors for the medium-voltage grid. A superconducting power cable running through the inner city of Essen passes a two-year field test; Supraleiter fuer das Mittelspannungsnetz. Ein supraleitendes Stromkabel quer durch die Essener Innenstadt besteht zweijaehrigen Feldtest

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, Franz

    2017-04-01

    Scientists are testing the longest high-temperature superconducting cable in the world under real conditions in Essen. One kilometre long, it connects two substations in the inner city. It replaces a conventional 110 kV line and renders one substation in the inner city obsolete. After two years of testing, it has passed the field test. It could be a blueprint for the future power supply system in urban areas. [German] Wissenschaftler testen in Essen das laengste Hochtemperatur-Supraleiterkabel der Welt unter realen Bedingungen. Mit einer Laenge von einem Kilometer verbindet es zwei Umspannstationen quer durch die Innenstadt. Es ersetzt eine konventionelle 110-kV-Leitung und macht eine Umspannanlage im Stadtzentrum ueberfluessig. In einer zweijaehrigen Erprobung hat es den Praxistest bestanden. Es koennte eine Blaupause fuer die kuenftige Stromversorgung in Ballungsraeumen sein.

  5. Water pollution potential of mineral oils with high content of polycyclic aromatic hydrocarbons (heavy fuel oil and neutral oil extracts); Untersuchungen zur Wassergefaehrdung durch Mineraloele mit hohen Gehalten an polycyclischen aromatischen Kohlenwasserstoffen (Heizoel Schwer und Extrakte)

    Energy Technology Data Exchange (ETDEWEB)

    Albers, G. [Mobil Schmierstoff GmbH, Hamburg (Germany)

    1999-01-01

    A data base on highly aromatic mineral oils has been compiled to classify mineral oil products according to their water-pollution potential (water hazard class or Wassergefaehrdungsklasse, WGK). This activity has been undertaken through the Commission for Water Hazardous Materials (Kommission Bewertung Wassergefaehrdender Stoffe, KBwS). In this special case, highly aromatic mineral oils containing a high concentration of polycyclic aromatic hydrocarbons (Polyaromatische Kohlenwasserstoffe, PAK) were evaluated. A test method for measuring the elution potential of PAK into water was developed on petroleum products with high viscosity and high freeze point. This method was applied to determine the solubility of 23 PAK (including 16 PAK according to EPA 610 and 6 PAK according to the German drinking water regulation (Trinkwasserverordnung, TVO)) from heavy fuel oil and neutral oil extract in the aqueous phase. For the 6 PAK, according to TVO, a sum limit of 0,2 {mu}g/l in drinking water is permitted by German legislation. This limit was not exceeded in any of the water phases examined. (orig.) [Deutsch] Fuer die Einstufung von Mineraloelprodukten in die Wassergefaehrdungsklassen (WGK) durch die Kommission Bewertung Wassergefaehrdender Stoffe ist es notwendig, Basisdaten zur Verfuegung zu stellen. Im speziellen Fall handelt es sich um die Bewertung von Mineraloelen, die sich durch einen hohen Gehalt an polycyclischen aromatischen Kohlenwasserstoffen (PAK) auszeichnen. Zur Eluierbarkeit von PAK`s aus Produkten mit hoher Viskosiaet bzw. mit hohem Stockpunkt wurde eine Pruefmethode entwickelt. Diese Methode wurde zur Bestimmung der Loeslichkeit von 23 PAK`s (16 PAK`s nach EPA-Liste incl. 6 PAK`s der TVO) aus den Mineraloelen Heizoel Schwer und Neutralextrakt in der Wasserphase eingesetzt. Fuer die PAK der TVO ist in der TVO ein Summengrenzwert von 0,2 {mu}g/l Trinkwasser angegeben. Dieser Grenzwert wurde in keiner der untersuchten Wasserphasen ueberschritten. (orig.)

  6. Radiation exposure to the patient caused by single-photon transmission measurement for 3D whole-body PET; Die Strahlenexposition des Patienten durch die Einzelphotonen-Transmissionsmessung bei der PET

    Energy Technology Data Exchange (ETDEWEB)

    Schaefer, A.; Donsch, P.; Kirsch, C.M. [Universitaet des Saarlandes, Homburg/Saar (Germany). Abt. fuer Nuklearmedizin; Seifert, H. [Universitaet des Saarlandes, Homburg/Saar (Germany). Abt. Strahlentherapie der Radiologischen Klinik

    2000-11-01

    Patienten durch die Transmissionsmessung mittels Einzelphotonenquellen bei der PET. Material und Methode: Zwei Cs-137-Punktquellen (E{gamma}=662 keV, A=614 MBq) werden zur Transmissionsmessung im Einzelphotonenmodus an einem 3D-Scanner (ECAT ART) eingesetzt. Bei der Simulation einer Ganzkoerper-Transmission (axiale Laenge: 75 cm, 6 ueberlappende Bettpositionen), werden Dosismessungen mit Thermolumineszenzdosimetern unter Verwendung eines Thorax- und eines Abdomenphantoms durchgefuehrt. Aus den Messwerten wurde in Anlehnung an den Report Nr. 60 der ICRP die effektive Dosis fuer die Transmissionsmessung abgeschaetzt. Ergebnisse: Bei einer Gesamtaufnahmedauer von 360 min (60 min pro Bettposition) ergaben sich folgende Energiedosen: Oberflaeche (Xyphoid) 189 {mu}Gy, Herz 196 {mu}Gy, Lunge 234 {mu}Gy, BWS 240 {mu}Gy, Niere 207 {mu}Gy, Leber 204 {mu}Gy, Gonaden 205 {mu}Gy, Schilddruese 249 {mu}Gy und Blase 185 {mu}Gy, aus denen sich ein Konversionsfaktor von 1,7*10{sup -4} mSv/(h*MBq) errechnete. Die Abschaetzung der effektiven Dosis fuer den Patienten aufgrund einer Transmissionsmessung (Akquisitionszeit von 3,2 min pro Bettposition) ergab einen Wert von 11 {mu}Sv. Die Abschaetzung des Verhaeltnisses der Konversionsfaktoren durch Transmissionsmessung im Einzelphotonen- und im Koinzidenzmodus (zwei Ge-68/Ga-68-Linienquellen mit jeweils 40 MBq) ergab einen Wert von 0,18. Der Vergleich zwischen den effektiven Dosen durch die Transmission im Einzelphotonen-Modus und die Emission (bei Injektion von 250 MBq FDG) ergab ein Verhaeltnis von 2,3*10{sup -3}. Schlussfolgerung: Die Strahlenexposition der Patienten durch die Transmissionsmessung in der 3D-PET ist vernachlaessigbar gering. Sie wird durch die Verwendung der Einzelphotonenmethode mit kollimierten Punktquellen relativ hoher Aktivitaet im Vergleich zur Koinzidenzmethode mit nicht-kollimierten Linienquellen relativ niedriger Aktivitaet weiter reduziert. (orig.)

  7. Studying Genes

    Science.gov (United States)

    ... NIGMS NIGMS Home > Science Education > Studying Genes Studying Genes Tagline (Optional) Middle/Main Content Area PDF Version (382 KB) Other Fact Sheets What are genes? Genes are segments of DNA that contain instructions ...

  8. Gene Therapy

    Science.gov (United States)

    Gene therapy Overview Gene therapy involves altering the genes inside your body's cells in an effort to treat or stop disease. Genes contain your ... that don't work properly can cause disease. Gene therapy replaces a faulty gene or adds a new ...

  9. Optimisation of the energy potential of low-CO2 gas turbines by using large monocrystal turbine blades. Final report; Optimierung des Energiepotentials fuer CO{sub 2}-arme Gasturbinen durch grosse Einkristall-Turbinenschaufeln. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Grossmann, J.

    2002-07-01

    Apart from the experimental identification and assessment of process characteristics during industrial-scale application of the so-called liquid metal cooling (LPC) process, the project focused on the characterisation of monocrystalline, directionally solidified materials and components. This involved the construction and commissioning of appropriate production and test aggregates, i.e. the LMC casting system and a Laue orientation testing unit, as well as the modification of a vacuum heat treatment furnace to meet the specific requirement of glow annealing of monocrystalline, directionally solidified turbine blades. [German] Das Ziel des Forschungsvorhabens (Laufzeit: 01. Juli 1997 bis 31. Dezember 2001) 'Optimierung des Energiepotentials fuer CO{sub 2}-arme Gasturbinen durch grosse Einkristall-Turbinenschaufeln' lag neben der experimentellen Bestimmung und Bewertung von Verfahrens- und Prozesskennwerten bei der industriellen Umsetzung des sogenannten Liquid-Metal-Cooling-Verfahrens (LMC) in der Eigenschaftscharakterisierung von einkristallin-gerichtet erstarrten Proben und Bauteilen, die mittels dieser neuartigen Giesstechnologie dargestellt wurden. Grundvoraussetzung fuer die umfassende Bearbeitung der o.g. Arbeitsinhalte war die Konstruktion und Inbetriebnahme der notwendigen Fertigungs- und Pruefaggregate, d.h. der LMC-Giessanlage und einer Laue-Orientierungspruefeinrichtung bzw. der Abstimmung eines Vakuum-Waermebehandlungsofens auf die spezifischen Anforderungen der Loesungsgluehung einkristallin-gerichtet erstarrter Turbinenlaufschaufeln. (orig.)

  10. Investigations into aerobic bacterial degradation of phenol compounds in consideration of ecological and physiological parameters; Untersuchungen zum aeroben Abbau phenolischer Verbindungen durch Bakterien unter Beruecksichtigung oekologisch und physiologisch relevanter Faktoren

    Energy Technology Data Exchange (ETDEWEB)

    Rittich, S.

    1992-07-01

    In a polluted soil of an abandoned gas works site, pure bacterial cultures were isolated which were able to degrade phenol, the three cresol isomers, and 2,3-, 2,4- and 3,4-xylenol. There were also mixed populations which degraded 2,5- and 3,5-xylenol. There were no bacteria in this soil which were able to degrade 2,6-xylenol. In spite of this, it was possible for the first time to decompose 2,6-xylenol using a mixed population of three different bacterial strains from other soils. One of these was a strain identified as Myeobacterium spec.SR18 which used 2,6-xylenol as its sole source of C and energy. (orig./EF). [Deutsch] Aus einem mit Schadstoffen kontaminierten Boden eines ehemaligen Gaswerkgelaeaendes konnten Reinkulturen isoliert werden, die in der Lage waren Phenol, die drei Kresolisomere und 2,3-, 2,4- und 3,4-Xylenol abzubauen. Ein 2,5- und 3,5-Xylenolabbau durch Mischpopulationen wurde ebenfalls beobachtet. Dagegen konnten aus diesem Boden keine 2,6-Xylenol-Abbauer gewonnen werden. Der bisher erst einmal beschriebene 2,6-Xylenolabbau gelang schliesslich mit einer weiteren, aus anderen verunreinigten Standorten angereicherten, Mischpopulation, die aus drei verschiedenen Bakterienstaemmen bestand. Darunter befand sich auch ein als Mycobacterium spec.SR18 identifizierter Stamm, der 2,6-Xylenol als alleinige C- und Energiequelle verwertet. (orig./EF).

  11. Partizipative medienpädagogische Aktionsforschung. Methodologische Überlegungen anlässlich einer Untersuchung der Medienkompetenz von und durch SchülerInnen an Neuen Mittelschulen in Wien aus Sicht des Theorie-Praxis-Problems.

    Directory of Open Access Journals (Sweden)

    Christian Swertz

    2015-12-01

    Full Text Available Für die Medienpädagogik ist wie für jede Pädagogik das Theorie-Praxis-Problem relevant. Im Beitrag wird partizipative Aktionsforschung als Methode zur Gestaltung von Theorie-Praxis-Korrelationen untersucht. Dazu wird zunächst das Theorie-Praxis-Problem als Relation der Korrelationen zwischen Theorie und Praxis in Forschungs- und Unterrichtsmethoden bestimmt. Anschließend wird durch Interpretationen von Theorien zur partizipativen Aktionsforschung und von Daten aus einem Projekt untersucht, ob die Relation von Forschung und Unterricht so gestaltet werden kann, dass zu souveränen Akten in solidarisch-libertären Gemeinschaften aufgefordert wird. Die Ergebnisse zeigen, dass dies möglich und die Methode also medienpädagogisch relevant ist. As for any education, the theory-practice transformation problem is relevant for media education. In this paper, participatory action research is investigated as a method to design the correlation of theory and practice. To do so, the theory-practice problem is understood as the relation of the correlations between theory and practice in research and teaching methods. Subsequently, it is researched if the relation of research and teaching can be designed to call for sovereign acts in solidary-libertarian communities. The research is conducted by interpreting participatory action research theories and project data. It is shown that it is possible to call for sovereign acts. Thus the suggested method can be considered as relevant for media education.

  12. gene structure, gene expression

    Indian Academy of Sciences (India)

    and seedling leaves were sampled at 6 h after the treatment. For cold stress, the seedlings were transferred to 4◦C growth chamber for 30 min. Control seedlings were exposed to none of these treatments. To examine the expression patterns of these predicted genes in Poplar and to further confirm their stress responsive-.

  13. Male gonadal dose an adjuvant 3-D-pelvic irradiation after anterior resection of rectal cancer. Influence to fertility; Radiogene Hodenbelastung durch Streustrahlung bei adjuvanter 3-D-Beckenbestrahlung nach anteriorer Resektion beim Rektumkarzinom. Einfluss auf die Fertilitaet

    Energy Technology Data Exchange (ETDEWEB)

    Piroth, M.D.; Hensley, F.; Wannenmacher, M.; Zierhut, D. [Radiologische Klinik der Univ. Heidelberg (Germany). Abt. fuer Klinische Radiologie

    2003-11-01

    Maenner erkranken. Wir untersuchten die radiogene Hodenbelastung durch Streustrahlung bei adjuvanter Beckenbestrahlung nach Operation eines Rektumkarzinoms. Die gemessenen Strahlendosen am Hoden wurden zu den Daten der Literatur in Beziehung gesetzt. Patienten und Methode: Wir fuehrten bei 18 Patienten mit thermolumineszenzdetektoren (TLD) In-vivo-Messungen zur Bestimmung der streustrahlenbedingten Gonadendosis durch. Die TLDs wurden an vier definierten Punkten des Skrotums fixiert. Die Bestrahlung erfolgte 3-D-geplant in Drei-Felder-Technik in Bauchlage im Lochbrett. Die Gesamtdosis betrug 50,4 Gy, die Einzeldosis 1,8 Gy taeglich. Nach 45 Gy erfolgte eine Modifikation der Bestrahlungstechnik auf opponierende und kranial verkuerzte Gegenfelder zur kompletten Duenndarmschonung. Ergebnisse: Die mittlere Gonadendosis aller 18 Patienten pro Bestrahlungsfraktion betrug 0,057 Gy (median 0,05 Gy), wobei die Mittelwerte der einzelnen Patienten zwischen 0,035 und 0,114 Gy lagen. Die Standardabweichung betrug 0,02 Gy. Nach 28 Fraktionen, 50,4 Gy entsprechend, errechnete sich kumulativ eine Gesamtdosis am Hoden von 1,60 Gy (0,98-3,19 Gy). Schlussfolgerungen: Das maennliche Keimepithel reagiert auf Bestrahlung ausserordentlich sensibel im Sinne eines negativen Fraktionierungseffektes. Bei Gesamtdosen > 1,5 Gy, fraktioniert appliziert, gilt die Azoospermie als meist irreversibel. Die von uns ermittelte Gonadendosis fuehrt nach den Daten der Literatur sehr wahrscheinlich zu einer dauerhaften Sterilitaet. Wegen der geringen Patientenanzahl sind die Daten vorsichtig zu interpretieren, dennoch muss der Patient ueber die hohe Wahrscheinlichkeit einer bleibenden Infertilitaet aufgeklaert werden. Eine moegliche praetherapeutische Spermakryokonservierung sollte mit dem Patienten besprochen werden. (orig.)

  14. Stabilization of the cleaning of anaerobic waste water with the aid of activated sludge following receipts of hydrogen peroxide; Stabilisierung der anaeroben Abwasserreinigung durch Belebtschlamm nach Eintrag von Wasserstoffperoxid

    Energy Technology Data Exchange (ETDEWEB)

    Stupperich, E. [Ulm Univ. (Germany). Abt. Angewandte Mikrobiologie; Gerstmeir, R. [Ulm Univ. (Germany). Abt. Angewandte Mikrobiologie; Marqua, J. [Ulm Univ. (Germany). Abt. Angewandte Mikrobiologie; Rothfuss, A. [Ulm Univ. (Germany). Abt. Angewandte Mikrobiologie

    1996-12-31

    Some municipal and industrial sewage treatment plants comprise an aerobic and an anaerobic unit. However, discharge conditions may be such as to cause strong oxidants like hydrogen peroxide (H{sub 2}O{sub 2}) to enter the anaerobic unit. As measurements of the reduction-oxidation potential in an acidification stage showed, small concentrations of H{sub 2}O{sub 2}, already, may cause irreversible damage to the anaerobic bacterial strains living there. Adding activated sludge from the aerobic unit to such a system can prevent the damage. Probably, the enzyme catalase protects the anaerobic system against the oxydizing effect of hydrogen peroxide: this enzyme from aerobic bacteria and optionally aerobic bacteria causes the ecologically compatible decomposition of hydrogen peroxide into water and oxygen, which is substantially less reactive. Optionally aerobic bacteria, which are also added, protect the anaerobic system further by consuming this oxygen. (orig.) [Deutsch] Manche kommunalen und industriellen Klaeranlagen bestehen aus einer Aerobie und einer Anaerobie. Verschiedene Einleitungsbedingungen koennen aber dazu fuehren, dass in die Anaerobie starke Oxidationsmittel wie Wasserstoffperoxid (H{sub 2}O{sub 2}) gelangen. Messungen des Redoxpotentials in einer Versaeuerungsstufe zeigen, dass bereits geringe Konzentrationen an H{sub 2}O{sub 2} die dort lebenden anaeroben Bakterienkulturen irreversibel schaedigen. Wird aber einem solchen System Belebtschlamm aus der Aerobie zudosiert, so kann diese Schaedigung verhindert werden. Wahrscheinlich schuetzt das Enzym Katalase die Anaerobie vor der oxidierenden Wirkung des Wasserstoffperoxids. Dieses Enzym aus aeroben und fakultativ aeroben Bakterien zersetzt naemlich Wasserstoffperoxid oekologisch vertraeglich in Wasser und den bedeutend weniger reaktiven Sauerstoff. Zum Schutz der Anaerobie wird auch dieser Sauerstoff durch die zudosierten fakultativ aeroben Bakterien verbraucht. (orig.)

  15. Reduction of construction cost and carbon dioxide emissions by heat contracting. Obstacles and solutions. Final report; Baukostensenkung und CO{sub 2}-Minderung durch Waerme-Contracting. Hemmnisse und Loesungswege. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Ebert, V.; Liebernickel, T.; Froemel, T.; Kues, H.

    2000-11-01

    The poor condition of many heating systems is caused by a conflict of interest between investors and users. Landlords tended to opt for low investment cost, while the accompanying higher consumption cost was paid by their tenants. The only thing that has changed here is the fact that the 'secondary cost' has become a criterion for new tenants' decision to rent an apartment. Heat contracting may be a way out of the problem as contractors are interested in high-efficiency heating systems and optimally controlled operation. Heat contracting is a service concept of private and communal organisations that involves payment, construction and operation of a heating system by the contractor depending on the contracting model used. [German] Die relativ schlechte und veraltete heizungstechnische Ausruestung der Wohngebaeude in Deutschland ist auf den lange Jahre bestehenden Interessenkonflikt zwischen Investor und Nutzer im Mietwohnungsbau zurueckzufuehren. Durch die Umlagefaehigkeit der Heizkosten auf die Nutzer (Mieter) war der Gebaeudeeigentuemer i.d.R. bestrebt, eine Anlage zu guenstigen Investitionskosten zu erwerben. Die daraus resultierenden, relativ hoeheren Verbrauchskosten waren als Betriebskosten auf die Mieter umlegbar und belasteten den Vermieter nicht. An dieser Situation hat sich bis heute prinzipiell nichts geaendert; allerdings sind die Nebenkosten mit der Hauptkostenposition 'Heizkosten' zu einem Kriterium der Vermietbarkeit geworden, so dass der Vermieter heute die Kosten moeglichst niedrig halten muss. Ein Ausweg aus dem Interessenkonflikt und der neuen Vermietsituation koennte das Waerme-Contracting sein. Seitens des Contractors ist der Einsatz hocheffizienter Anlagentechnik und optimale, d.h. ueberwachte Betriebsbedingungen fuer einen wirtschaftlichen Erfolg unabdingbar. Waerme-Contracting ist ein Dienstleistungskonzept von privatwirtschaftlichen und kommunalen Unternehmen, die - je nach Contracting

  16. Durch Kooperation und Vernetzung zu neuen Aufgabenfeldern – Serviceentwicklung in der Bibliothek der Medizinischen Fakultät Mannheim [Opening up new tasks through cooperation and networking – service development in the Library for the Medical Faculty of Mannheim

    Directory of Open Access Journals (Sweden)

    Semmler-Schmetz, Martina

    2013-09-01

    Full Text Available [english] The Library for the Medical Faculty of Mannheim of the University of Heidelberg supplies literature and information for research, teaching and further education purposes at the “Universitätsmedizin Mannheim”. Cooperation and networking with the central facilities, departments, working groups, and information multipliers result in new, future-oriented areas of work that go beyond traditional library tasks. New services for students focus primarily on academic research and writing skills, while information and consulting services which are flexible in terms of time and location are being developed for staff members of the clinical departments and institutes. The recent cooperation with actors in the field of evidence-based medicine has yielded the innovative service “systematic literature search” which is designed to support doctors and scientists in the preparation of grant proposals for clinical trials, systematic reviews, and guidelines. Currently, the library is widening access to its new services to include doctors, scientists, and other health practitioners in the region, and evaluating chargeable services for these user groups.[german] Die Bibliothek der Medizinischen Fakultät Mannheim der Universität Heidelberg dient der Literatur- und Informationsversorgung für Forschung, Lehre, Fort- und Weiterbildung an der Universitätsmedizin Mannheim. Neue, zukunftsorientierte Aufgabenfelder, die über die klassischen bibliothekarischen Arbeitsschwerpunkte hinausgehen, erschließt sich die Bibliothek durch enge Kooperation und Vernetzung mit den zentralen Einrichtungen und Abteilungen, Arbeitskreisen und Multiplikatoren innerhalb der Universitätsmedizin Mannheim. Neue Services für die Zielgruppe der Studierenden richten sich vor allem auf den Bereich des wissenschaftlichen Arbeitens, während für Mitarbeiter in den Einrichtungen, Kliniken und Instituten der Universitätsmedizin zeitlich und räumlich flexible Informations

  17. Optimised utilisation of existing incinerators by installation of upstream reactors for treatment of waste with high calorifica value - HYBRID waste treatment plants; Optimierte Nutzung bestehender Abfallverbrennungsanlagen durch Errichtung vorgeschalteter Reaktoren zur Behandlung heizwertreicher Abfaelle - HYBRID-Abfallbehandlungsanlagen

    Energy Technology Data Exchange (ETDEWEB)

    El Labani, M.

    2000-07-01

    Abfallverbrennungsanlagen nachtraeglich um leistungsfaehige Rauchgasreinigungssysteme ergaenzt werden. Dies fuehrte zu einem erheblichen Anstieg der Kosten und Behandlungsgebuehren mit dem entsprechenden Druck zur optimierten Nutzung. Zusaetzliche Behandlungskapazitaeten werden benoetigt fuer heizwertreiche Abfaelle. Sie koennen zwar in konventionellen Abfallverbrennungsanlagen fuer Hausmuell behandelt werden, bedingen aber aufgrund des hoeheren Heizwertes eine Durchsatzreduzierung was die Kostensituation weiter negativ beeinflusst. Daher favorisieren aktuelle Konzepte die Behandlung heizwertreicher Abfaelle in spezifischen, dezentralen Anlagen. Die ueblicherweise kleinen Anlagen gehen einher mit hohen Nebenkosten der Errichtung. Zur Abschaetzung des Optimierungspotentials bestehender Abfallverbrennungsanlagen wurde deren Auslastungssituation untersucht. Bei umfangreichen Messkampagnen am MHKW Darmstadt konnte selbst bei 100%iger Auslastung der Verbrennungseinheit in der Rauchgasreinigungsanlage eine Luecke aufgezeigt werden, die durch eine entsprechend angepasste Vorschaltanlage aufgefuellt werden koennte. Eine solche Vorschaltanlage definiert zusammen mit der bestehenden Abfallverbrennungsanlage eine HYBRID-Abfallbehandlungsanlage. Die Bereitstellung eines Instrumentariums zur Unterstuetzung der Entscheidungsfindung bei der Konzeption solcher Anlagen ist Gegenstand dieser Arbeit. (orig.)

  18. Gene expression

    International Nuclear Information System (INIS)

    Hildebrand, C.E.; Crawford, B.D.; Walters, R.A.; Enger, M.D.

    1983-01-01

    We prepared probes for isolating functional pieces of the metallothionein locus. The probes enabled a variety of experiments, eventually revealing two mechanisms for metallothionein gene expression, the order of the DNA coding units at the locus, and the location of the gene site in its chromosome. Once the switch regulating metallothionein synthesis was located, it could be joined by recombinant DNA methods to other, unrelated genes, then reintroduced into cells by gene-transfer techniques. The expression of these recombinant genes could then be induced by exposing the cells to Zn 2+ or Cd 2+ . We would thus take advantage of the clearly defined switching properties of the metallothionein gene to manipulate the expression of other, perhaps normally constitutive, genes. Already, despite an incomplete understanding of how the regulatory switch of the metallothionein locus operates, such experiments have been performed successfully

  19. Health injuries by noise; Gesundheitsgefahren durch Laerm

    Energy Technology Data Exchange (ETDEWEB)

    Plath, P. [Klinik fuer Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie, Bochum Univ., Prosper-Hospital, Recklinghausen (Germany)

    1995-07-01

    Within the civilized world, noise is a common danger for health. There is not only the well known hearing loss by occupational noise, but there are also a lot of noise induced hearing losses by noise injuries in private life which are comparable to the occupational ones. The sources of these injuries within private life mostly are unknown and can be found in household goods as well as from listening to loud music. Also the danger from loud cracks mostly is underestimated, so as the cracks from children toys often reach more than 150 dB(Al). Alladay`s noise levels, e.g. in public trafic, also reach dangerous levels, and the sum of all these noise injuries results in a lesion of the cilias of the outer hair cells in the Corti`s organ. The resulting hearing loss is called `sociacusis` and increases the effect of physiological presbyacusis to an amount that results in the fact, that more than 10% of all adults in civilized countries have a severe, handicapping deafness. The immediate effect of noise in this regard is intensified by the effects of disturbing noise onto the psychovegetative and hormonal regulations by chronical stress. So people in civilized countries are going to become a society of hard hearing subjects, and deafness will become a common peoples` disease. (orig.) [Deutsch] Laerm ist in der zivilisierten Welt ein allgemeines Gesundheitsproblem. Es gibt nicht nur die berufliche Laermschwerhoerigkeit, die gut erforscht ist, sondern schaedlicher Laerm tritt sehr oft auch im Privatleben auf und verursacht Gehoerschaeden, die der beruflich bedingten Laermschwerhoerigkeit vergleichbar sind. Die Gehoergefaehrdung im Privatleben ist oft unbekannt und betrifft neben lauten Geraeten im Haushalt und dem Hoeren zu lauter Musik auch die oft in ihrer Gefaehrlichkeit unterschaetzten Knalle, die selbst bei Kinderspielzeug Spitzenwerte der Pegel von ueber 150 dB(Al) erreichen. Die allgemeine zivilisatorische Laermbelastung, z.B. im oeffentlichen Verkehr, die oft schaedigende Ausmasse erreicht, im Beurteilungspegel aber unter den Grenzwerten bleibt, bewirkt im Laufe der Jahre ebenfalls bleibende Schaeden an der Zilien der aeusseren Haarzellen des Corti-Organs, die eine ueber die physiologische Alterung hinausgehende `Soziakusis` bewirken. Schliesslich haben die staendigen Geraeusch- und Laermbelaestigungen und -stoerungen des Alltagslebens deutliche Effekte auf die neurovegetativen und hormonellen Regulationen des Organismus und verursachen somit mittelbar ueber Stressreaktionen ebenfalls Hoerstoerungen in Form von Hoerstuerzen und Tinnitus. Der alltaegliche Laerm, dem zu wenig Bedeutung zugemessen wird, droht Schwerhoerigkeit, von der mehr als 10% aller Erwachsenen betroffen sind, zur Volkskrankheit werden zu lassen. (orig.)

  20. Internationale Wachstumsstrategien produzierender Familienunternehmen durch Mergers & Acquisitions

    NARCIS (Netherlands)

    Sachs, Andreas Fabian Constantin Johannes

    2007-01-01

    Family businesses are experiencing a revival in the German economy. In the face of the challenges of globalization and the ongoing relocation of production facilities, “home base oriented” enterprises meet with increasing attention and gain in importance. Family-owned enterprises have indeed been

  1. Safety by simulation; Sicherheit durch Simulation

    Energy Technology Data Exchange (ETDEWEB)

    Hoffmann, Eberhard [KSG Kraftwerks-Simulator-Gesellschaft mbH, Essen (Germany); GfS Gesellschaft fuer Simulatorschulung mbH, Essen (Germany)

    2009-06-15

    Simulator training is a key component in achieving and preserving on the long term the necessary expertise of plant personnel also required by the authorities. In this way, it makes an important contribution to the safe operation of nuclear power plants. Simulators are a component in the training of operating personnel of nuclear power plants which allows nuclear power plant operators to be prepared in a focused and practice-oriented way for their activity in everyday plant operation and for possible accident simulation. The simulator center is supported by 5 nuclear power plant operators: the German E.ON Kernkraft GmbH, RWE Power AG, EnBW Kraftwerke AG, and Vattenfall Europe Nuclear Energy GmbH companies as well as the Netherlands N.V. Electriciteits-Produktiemaatschappij Zuid-Nederland. They established a joint enterprise in Essen which performs in one central place the duty of simulator training incumbent upon all nuclear power plants. (orig.)

  2. Erkenntnisgewinnn durch Virtuelle Realitäten

    Directory of Open Access Journals (Sweden)

    Breiner, Tobias

    2009-11-01

    Full Text Available Virtual reality will gain a more and more important role in our everyday life. So it is very important to understand fully the impact and influence virtual reality will have on or view of the world. This article reveals that the interdependencies between the real space and the virtual space are much more subtle and profound then on first sight. Especially the value of several mathematical, physical and biological models and simulation methodologies will be reestimated by the use of virtual reality in the long term. As an example the article describes the historical chain from quaternions to quaoaring and how this may lead to a paradigm change in biology.

  3. Trichoderma genes

    Science.gov (United States)

    Foreman, Pamela [Los Altos, CA; Goedegebuur, Frits [Vlaardingen, NL; Van Solingen, Pieter [Naaldwijk, NL; Ward, Michael [San Francisco, CA

    2012-06-19

    Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.

  4. „Wir verbrachten mehr als 24 Stunden, ohne etwas anderes als Schokolade und Limonade zu uns zu nehmen“. Hinweise in Alexander von Humboldts Tagebuchaufzeichnungen zu Fragen der Verpflegung auf der Forschungsreise durch Spanisch-Amerika

    Directory of Open Access Journals (Sweden)

    Ursula Thiemer-Sachse

    2013-12-01

    Full Text Available Zusammenfassung In Alexander von Humboldts Tagebüchern seiner berühmten Forschungsreise durch Spanisch-Amerika finden sich Bemerkungen und Hinweise für künftige Leser seiner Reisedarstellungen zu Problemen der Verpflegung. Zumeist werden Engpässe und Missstände erwähnt sowie Fragen soziopolitischer Situation verdeutlicht. Sie beziehen sich auf Extrembedingungen in stadtfernen Gebieten und im Kontakt mit den indigenen Trägern und Helfern während der einzelnen Exkursionen. Die Darstellungen der alltäglichen Gegebenheiten sind zugleich eine interessante Quelle für Verhaltensmuster der verschiedenen Schichten der spanischkolonialen Gesellschaft wie der Forschungsreisenden, die mit den Schwierigkeiten umzugehen hatten, sich anzupassen verstanden oder ihre eigenen Verhaltensmuster entwickelt haben. Damit ermöglichen Humboldts Aufzeichnungen zugleich auch auf diesem Gebiet manchen Blick auf den seither erfolgten Kulturwandel. Resumen En los diarios del famoso viaje de estudios de Alejandro de Humboldt por la América española se encuentran observaciones y notas sobre problemas de aprovisionamiento, en favor de lectores futuros de sus relaciones de viaje. En la mayoría de los casos Humboldt menciona situaciones precarias o ilustra cuestiones de carácter sociopolítico. Frecuentemente, sus apuntes se refieren a condiciones extremas en regiones remotas y en contacto con los cargadores y ayudantes indígenas durante las diferentes excursiones. Las descripciones de los acontecimientos cotidianos al mismo tiempo son una fuente interesante sobre los modos de proceder de las diferentes capas de la sociedad colonial española así como de los viajeros científicos que tuvieron que enfrentar dificultades a las cuales había que adaptarse o desarrollar modelos propios de comportamiento. Por esto al mismo tiempo las noticias de Humboldt también en este asunto posibilitan más de una vez la atención al cambio cultural que se ha realizado hasta hoy

  5. Immunoglobulin genes

    National Research Council Canada - National Science Library

    Honjo, T; Alt, F. W; Rabbitts, T. H

    1989-01-01

    ... Cataloguing in Publication Data Immunoglobulin genes 1. Vertebrates. Immunoglobulins 1. Honjo, T. II. Alt, F.W. III. Rabbitts, T.H. 612'. 118223 ISBN 0-12-354865-9 This book is printed on acid-free paper ( T...

  6. Ageing genes

    DEFF Research Database (Denmark)

    Rattan, Suresh

    2018-01-01

    The idea of gerontogenes is in line with the evolutionary explanation of ageing as being an emergent phenomenon as a result of the imperfect maintenance and repair systems. Although evolutionary processes did not select for any specific ageing genes that restrict and determine the lifespan...... of an individual, the term ‘gerontogenes’ primarily refers to any genes that may seem to influence ageing and longevity, without being specifically selected for that role. Such genes can also be called ‘virtual gerontogenes’ by virtue of their indirect influence on the rate and process of ageing. More than 1000...... virtual gerontogenes have been associated with ageing and longevity in model organisms and humans. The ‘real’ genes, which do influence the essential lifespan of a species, and have been selected for in accordance with the evolutionary life history of the species, are known as the longevity assurance...

  7. Characterization of clouds and aerosols by lidar remote sensing with regard to the transfer of UV radiation. Final report; Leitthema 4: Solare UV-B-Strahlung. Teilvorhaben: Charakterisierung von Wolken und Aerosolen durch Lidar-Fernerkundung hinsichtlich des UV-Strahlungstransfers. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Jaeger, H.; Muecke, R.; Kreipl, S.

    2000-05-01

    The investigations characterize aerosols and clouds over Garmisch-Partenkirchen by lidar remote sensing, and support the interpretation of measurements of the transfer of UV radiation. Extensive tests of calibration procedures were performed, and the lidar system was modified to meet the requirements. The focal point were measurements accompanying the CUVRA (Characteristics of the UV Radiation field in the Alps) campaign at IFU in March 1999. 767 lidar measurements in two wavelength channels were performed on 12 days during this campaign. Aerosol and cloud situations were characterized by determining lower and upper layer heights (time resolution down to 1 minute, height resolution down to 15 meters), multi layer situations from ground to the tropopause region (up to 5 aerosol and cloud layers), optical depths at 532 nm and 355 nm (range 0.01 to 3), wavelength dependences of the optical depth between 532 nm and 355 nm (range of wavelength exponent 0 to -2), and lidar ratios (extinction/backscatter) at 532 nm (range 20 to 38). (orig.) [German] Die Untersuchungen charakterisieren Aerosole und Wolken ueber Garmisch-Partenkirchen durch Lidar-Fernerkundung und dienen der Interpretation von Messungen des UV-Strahlungsflusses. Hierzu waren umfangreiche Arbeiten zum Testen von Eichverfahren notwendig, ebenso wie Modifizerungen des Lidarsystems. Schwerpunkt der Untersuchungen waren Messungen waehrend der CUVRA (Characteristics of the UV Radiation field in the Alps) Messkampagne im Maerz 1999 am IFU. Waehrend dieser Kampagne wurden an 12 Messtagen 767 Lidarmessungen in jeweils zwei Wellenlaengenkanaelen durchgefuehrt. Die Aerosol- und Wolkensituationen wurden charakterisiert durch die Bestimmung von Schichtunter- und obergrenzen (Zeitaufloesung bis zu 1 Minute, Hoehenaufloesung bis zu 15 m), der Mehrschichtigkeit vom Boden bis zum Tropopausenbereich (bis zu 5 Aerosol- und Wolkenschichten), der optischen Dicken bei 532 nm und 355 nm (Bereich 0.01 bis 3), der

  8. Gene Locater

    DEFF Research Database (Denmark)

    Anwar, Muhammad Zohaib; Sehar, Anoosha; Rehman, Inayat-Ur

    2012-01-01

    software's for calculating recombination frequency is mostly limited to the range and flexibility of this type of analysis. GENE LOCATER is a fully customizable program for calculating recombination frequency, written in JAVA. Through an easy-to-use interface, GENE LOCATOR allows users a high degree...... of flexibility in calculating genetic linkage and displaying linkage group. Among other features, this software enables user to identify linkage groups with output visualized graphically. The program calculates interference and coefficient of coincidence with elevated accuracy in sample datasets. AVAILABILITY......: The database is available for free at http://www.moperandib.com....

  9. 11. Frankfurter Medienrechtstage 2013 "Internet - Segen oder Fluch für die Medienfreicheit in Europa?" : 20-21. März 2013, Europa-Universität Viadrina, Frankfurt (Oder)

    Index Scriptorium Estoniae

    2013-01-01

    Terve ajakirja number on pühendatud konverentsi materjalidele. Muutustest meedias ja selle mõjust trükiajakirjandusele ja ajakirjandusvabadusele. Blogidest, nende tähendusest ning vajalikkusest. Ajakirjanike ja blogide rollist Ukrainas ja Venemaal. Autoriõigus ja digitaalsed väljaanded, digitaalmeedia finantseerimise küsimused

  10. Genes and Hearing Loss

    Science.gov (United States)

    ... ENTCareers Marketplace Find an ENT Doctor Near You Genes and Hearing Loss Genes and Hearing Loss Patient ... mutation may only have dystopia canthorum. How Do Genes Work? Genes are a road map for the ...

  11. Design criteria for crankshafts - axial slide bearing in Otto and diesel engines by means of computer-based simulations and experimental investigations. Axial slide bearing design - final report; Auslegungskriterien fuer Kurbelwellen - Axialgleitlager in Otto- und Dieselmotoren durch rechnergestuetzte Simulation und experimentelle Untersuchungen. Axialgleitlagerauslegung - Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Backhaus, K. [Univ. GH Kassel (Germany). Inst. fuer Maschinenelemente und Konstruktionstechnik; Hunsicker, W. [Fachhochschule Mannheim (Germany). Inst. fuer Tribologie; Schubert, W. [KS Gleitlager GmbH, St. Leon-Rot (Germany)

    2003-07-01

    In the frame of this research project a simulation program has been developed in order to investigate suspension pressure build-up in crankshaft axial bearings taking into account mixed friction conditions. The investigated system is performing in a dynamic way taking into account the engine dynamics. Based upon the high engineering state of radial slide bearings, axial bearings as well as conical slide faces with any conical angle can be simulated in the same way. The solid body contact share in the system to be investigated is taken into account by the integral effect of the real microscopic surface structure on microhydrodynamics and contact pressure build-up. The influence of individual parameters, as e.g. macro-geometric bearing surface design can be investigated by means of simulation results. Wavy stopping faces and bearing surfaces with Rayleigh-steps are characterised by high hydrodynamic supporting pressure magnitudes in terms of macrogeometry. Elasto-hydrodynamic investigations at one stop collar and under engine-specific load on stop disks of a base bearing show the influence of structure elasticity and prove the necessity to consider them when the individual systems are analysed. (orig.) [German] Im Rahmen des durchgefuehrten Forschungsvorhabens wurde ein Simulationsprogramm zur Untersuchung des Tragdruckaufbaus in Kurbelwellen-Axiallagern unter Beruecksichtigung von Mischreibungszustaenden entwickelt. Das untersuchte System verhaelt sich dabei unter Beruecksichtigung der Motordynamik dynamisch. Ausgehend von dem hohen Entwicklungsstand von Radialgleitlagern koennen Axiallager sowie kegelfoermige Gleitflaechen mit beliebigen Kegelwinkeln in gleicher Weise simuliert werden. Dem hohen Festkoerperkontaktanteil in dem zu untersuchenden System wird durch die Beruecksichtigung der integralen Wirkung der realen mikroskopischen Oberflaechenstruktur auf Mikrohydrodynamik und Kontaktdruckaufbau Rechnung getragen. Anhand von Simulationsergebnissen kann der

  12. Gene expression and gene therapy imaging

    International Nuclear Information System (INIS)

    Rome, Claire; Couillaud, Franck; Moonen, Chrit T.W.

    2007-01-01

    The fast growing field of molecular imaging has achieved major advances in imaging gene expression, an important element of gene therapy. Gene expression imaging is based on specific probes or contrast agents that allow either direct or indirect spatio-temporal evaluation of gene expression. Direct evaluation is possible with, for example, contrast agents that bind directly to a specific target (e.g., receptor). Indirect evaluation may be achieved by using specific substrate probes for a target enzyme. The use of marker genes, also called reporter genes, is an essential element of MI approaches for gene expression in gene therapy. The marker gene may not have a therapeutic role itself, but by coupling the marker gene to a therapeutic gene, expression of the marker gene reports on the expression of the therapeutic gene. Nuclear medicine and optical approaches are highly sensitive (detection of probes in the picomolar range), whereas MRI and ultrasound imaging are less sensitive and require amplification techniques and/or accumulation of contrast agents in enlarged contrast particles. Recently developed MI techniques are particularly relevant for gene therapy. Amongst these are the possibility to track gene therapy vectors such as stem cells, and the techniques that allow spatiotemporal control of gene expression by non-invasive heating (with MRI guided focused ultrasound) and the use of temperature sensitive promoters. (orig.)

  13. Imaging reporter gene for monitoring gene therapy

    International Nuclear Information System (INIS)

    Beco, V. de; Baillet, G.; Tamgac, F.; Tofighi, M.; Weinmann, P.; Vergote, J.; Moretti, J.L.; Tamgac, G.

    2002-01-01

    Scintigraphic images can be obtained to document gene function at cellular level. This approach is presented here and the use of a reporter gene to monitor gene therapy is described. Two main ways are presented: either the use of a reporter gene coding for an enzyme the action of which will be monitored by radiolabeled pro-drug, or a cellular receptor gene, the action of which is documented by a radio labeled cognate receptor ligand. (author)

  14. Risks and opportunities of the liberalized electricity market. Partial project: BoFiT, an integrated decision support system for retaining the competitiveness of the cogeneration technology. Final report; Risiken und Chancen des liberalisierten Strommarktes. Teilprojekt: Integrierte Entscheidungsunterstuetzung durch BoFiT zur Erhaltung der Wettbewerbsfaehigkeit der Kraft-Waerme-Kopplung. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Stock, G.; Scheidt, M.

    2002-06-04

    The energy management system called BoFiT and specific new applications are explained which have been developed as one project task of the coordinated research project sponsored by the German ministry of economics,(BMWi), entitled ''Risks and opportunities of the liberalized electricity market''. The major objective of the project is: Finding efficient strategies to ensure the competitiveness of the cogeneration technology in the deregulated power and gas markets, in particular for the purpose of enhancing the penetration of the ecologically beneficial, cogenerated district heating supply in those markets. The focus of this report is on a specific application of the integrated BoFiT decision support system, for which the ''model for microsimulation of spot transactions at the Power Exchange based on a multi-agent system'' has been developed and is explained in great detail. (orig./CB) [German] Mit dem Energiemanagementsystem BoFiT soll den Unternehmen eine effektive und operativ nutzbare Entscheidungsunterstuetzung angeboten werden. Wesentliche Ziele des Verbundprojektes des BMWi sind: Unterstuetzung der KWK und speziell der darauf basierenden oekologisch sinnvollen Fernwaermeversorgung durch Anpassung von Arbeitsablaeufen und Werkzeugen zur Findung betriebswirtschaftlich optimaler Einsatz- und Betriebsstrategien; Kostenoptimierung der Kraftwerke, Vertraege und Stromhandelsaktivitaeten unter den Randbedingungen der deregulierten Strom- und Gasmaerkte. Als Beispiel einer solchen integrierten Entscheidungsunterstuetzung wird das ''Modell zur Mikrosimulation des Spothandels von Strom auf der Basis eines Multi-Agenten-Systems'' ausfuehrlich beschrieben. (orig./CB)

  15. How Genes Evolve

    Indian Academy of Sciences (India)

    which they are found e.g. the evolution of the gene coding for the protein cytochrome-C which is part ofthe respiratory apparatus. On the contrary, paralogous genes are descendants of a duplicated gene. Paralogous genes therefore evolve within the same species as well as in different species e.g. genes coding for alpha ...

  16. Regulation of FoxO transcription factors by environmental NO(x). Influence of metal ions and polycyclic aromatic hydrocarbons; Regulation von FoxO-Transkriptionsfaktoren durch Umweltnoxen. Einfluss von Metallionen und polyzyklischen aromatischen Kohlenwasserstoffen

    Energy Technology Data Exchange (ETDEWEB)

    Eckers, Anna

    2009-12-15

    FoxO transcription factors are crucial modulators of various cellular processes, controlling the expression of target genes such as those coding for manganese superoxide dismutase (MnSOD) and selenoprotein P (SeP), thereby supporting defense against oxidative stress. Environmental stimuli such as heavy metal ions and polycyclic aromatic hydrocarbons (PAH) modulate signaling pathways both by interaction with proteins or by inducing the generation of reactive oxygen species (ROS). Exposure of hepatoma cells to nickel ions at subcytotoxic doses did not translate into modulation of FoxO activity despite an activation of the Ser/Thr-kinase Akt. The cellular response to nickel ions under these conditions is most likely independent of the formation of ROS, since there were no increased levels of glutathione disulfide detectable. FoxO activity was then found to be modulated in response to exposure of cells to PAH or the tryptophan photoproduct FICZ. Both PAH and FICZ caused an increased activity of a FoxO-responsive promoter construct as well as of glucose 6-phosphatase promoter activity. In contrast, the activities of promoters of genes coding for MnSOD or SeP were decreased in response to exposure to the PAH 3-methylcholanthrene (3-MC). In line with the promoter effects, 3-MC also decreased steady-state levels of SeP mRNA. The response of the SeP promoter to 3-MC was abrogated by point mutations introduced at the two identified FoxO binding elements of the SeP promoter, implying that interaction of FoxO proteins with these sites is essential for the downregulation of promoter activity. In addition to FoxO activity being modulated by xenobiotics, it was then demonstrated that FoxO expression was also modulated by exposure of cells to PAH or FICZ. FoxO4 mRNA levels were downregulated in hepatoma cells exposed to 3-MC or FICZ. Similarly, insulin treatment caused a downregulation of mRNA levels of FoxO 1a, 3a and 4 in hepatoma cells. (orig.)

  17. Imaging gene expression in gene therapy

    International Nuclear Information System (INIS)

    Wiebe, Leonard I.

    1997-01-01

    Full text. Gene therapy can be used to introduce new genes, or to supplement the function of indigenous genes. At the present time, however, there is non-invasive test to demonstrate efficacy of the gene transfer and expression processes. It has been postulated that scintigraphic imaging can offer unique information on both the site at which the transferred gene is expressed, and the degree of expression, both of which are critical issue for safety and clinical efficacy. Many current studies are based on 'suicide gene therapy' of cancer. Cells modified to express these genes commit metabolic suicide in the presence of an enzyme encoded by the transferred gene and a specifically-convertible pro drug. Pro drug metabolism can lead to selective metabolic trapping, required for scintigraphy. Herpes simplex virus type-1 thymidine kinase (H S V-1 t k + ) has been use for 'suicide' in vivo tumor gene therapy. It has been proposed that radiolabelled nucleosides can be used as radiopharmaceuticals to detect H S V-1 t k + gene expression where the H S V-1 t k + gene serves a reporter or therapeutic function. Animal gene therapy models have been studied using purine-([ 18 F]F H P G; [ 18 F]-A C V), and pyrimidine- ([ 123 / 131 I]I V R F U; [ 124 / 131I ]) antiviral nucleosides. Principles of gene therapy and gene therapy imaging will be reviewed and experimental data for [ 123 / 131I ]I V R F U imaging with the H S V-1 t k + reporter gene will be presented

  18. Dexamethasone-induced radioresistance occurring independent of human papilloma virus gene expression in cervical carcinoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Rutz, H.P.; Mariotta, M.; Mirimanoff, R.O. [Lab. de Radiobiologie, Service de Radio-Oncologie, CHUV, Lausanne (Switzerland); Knebel Doeberitz, M. von [Deutsches Krebsforschungszentrum, Heidelberg (Germany). Inst. fuer Virusforschung

    1998-02-01

    The aim of this study was to investigate the role of HPV 18 E6 and E7 gene products with respect to radiosensitivity of two cervical carcinoma cell lines. The two cervical carcinoma lines C4-1 and SW 756 were used in which treatment with dexamethasone allows to modulate expression levels of HPV 18 E6 and E7 genes: Upregulation in C4-1, down-regulation in SW 756. Effects of treatment with dexamethasone on plating efficiency and radiosensitivity were assessed using a clonogenic assay. Treatment with dexamethasone increased plating efficiency of the C4-1 cells, but did not affect plating efficiency of SW 756 cells. Treatment with dexamethasone induced enhanced radioresistance in both cell lines. Thus, in C4-1 cells the observed changes in radioresistance correlate to the enhancement in expression of HPV 18 genes E6/E7, whereas in SW 756, a reduced expression correlates negatively with the enhanced radioresistance. (orig./MG) [Deutsch] Das Ziel dieser Studie lag darin, die Rolle der HPV-18-Gene E6 und E7 in bezug auf die Strahlenempfindlichkeit von menschlichen Zervixkarzinomzellen zu untersuchen. Wir verwendeten zwei menschliche Zervixkarzinomzellinien, C4-1 und SW 756, in welchen die Expression der viralen Gene HPV 18 E6 und E7 mit Dexamethason moduliert werden kann: In C4-1 bewirkt die Behandlung mit Dexamethason eine Erhoehung der Expression dieser Gene, in SW 756 eine Verminderung. Die Wirkung auf die Wachstumsfaehigkeit der Zellen und auf die Wachstumshemmung durch die Bestrahlung wurde unter Verwendung eines klonogenen Assays bestimmt. Dexamethason bewirkte eine erhoehte Wachstumsfaehigkeit der C4-1 Zellen, ohne die Wachstumsfaehigkeit der SW-756-Zellen zu beeinflussen, wie schon frueher beschrieben. Die Resistenz beider Zellinien gegenueber Bestrahlung wurde erhoeht. Somit besteht in den C4-1-Zellen eine Korrelation der Expression der viralen Gene mit der Zunahme der Strahlenresistenz, wogegen in den SW-756-Zellen die Abnahme der Expression im Gegensatz zu

  19. Gene cluster statistics with gene families.

    Science.gov (United States)

    Raghupathy, Narayanan; Durand, Dannie

    2009-05-01

    Identifying genomic regions that descended from a common ancestor is important for understanding the function and evolution of genomes. In distantly related genomes, clusters of homologous gene pairs are evidence of candidate homologous regions. Demonstrating the statistical significance of such "gene clusters" is an essential component of comparative genomic analyses. However, currently there are no practical statistical tests for gene clusters that model the influence of the number of homologs in each gene family on cluster significance. In this work, we demonstrate empirically that failure to incorporate gene family size in gene cluster statistics results in overestimation of significance, leading to incorrect conclusions. We further present novel analytical methods for estimating gene cluster significance that take gene family size into account. Our methods do not require complete genome data and are suitable for testing individual clusters found in local regions, such as contigs in an unfinished assembly. We consider pairs of regions drawn from the same genome (paralogous clusters), as well as regions drawn from two different genomes (orthologous clusters). Determining cluster significance under general models of gene family size is computationally intractable. By assuming that all gene families are of equal size, we obtain analytical expressions that allow fast approximation of cluster probabilities. We evaluate the accuracy of this approximation by comparing the resulting gene cluster probabilities with cluster probabilities obtained by simulating a realistic, power-law distributed model of gene family size, with parameters inferred from genomic data. Surprisingly, despite the simplicity of the underlying assumption, our method accurately approximates the true cluster probabilities. It slightly overestimates these probabilities, yielding a conservative test. We present additional simulation results indicating the best choice of parameter values for data

  20. Essential Bacillus subtilis genes

    NARCIS (Netherlands)

    Kobayashi, K.; Ehrlich, S.D.; Albertini, A.; Amati, G.; Andersen, K.K.; Arnaud, M.; Asai, K.; Ashikaga, S.; Aymerich, S.; Bessieres, P.; Boland, F.; Brignell, S.C.; Bron, S; Bunai, K.; Chapuis, J; Christiansen, L.C.; Danchin, A.; Debarbouille, M.; Dervyn, E.; Deuerling, E.; Devine, K.; Devine, S.K.; Dreesen, O.; Errington, J.; Fillinger, S.; Foster, S.J.; Fujita, Y.; Galizzi, A.; Gardan, R.; Eschevins, C.; Fukushima, T.; Haga, K.; Harwood, C.R; Hecker, M.; Hosoya, D.; Hullo, M.F.; Kakeshita, H.; Karamata, D.; Kasahara, Y.; Kawamura, F.; Koga, K.; Koski, P.; Kuwana, R.; Imamura, D.; Ishimaru, M.; Ishikawa, S.; Ishio, I.; Le Coq, D.; Masson, A.; Mauel, C.; Meima, Roelf; Mellado, R.P.; Moir, A.; Moriya, S.; Nagakawa, E.; Nanamiya, H.; Nakai, S.; Nygaard, P.; Ogura, M.; Ohanan, T.; O'Reilly, M.; O'Rourke, M.; Pragai, Z.; Pooley, H.M.; Rapoport, G.; Rawlins, J.P.; Rivas, L.A.; Rivolta, C.; Sadaie, A.; Sadaie, Y.; Sarvas, M; Sato, T.; Saxild, H.H.; Scanlan, E.; Schumann, W; Seegers, J.F. M. L.; Sekiguchi, J.; Sekowska, A.; Seror, S.J.; Simon, M.; Stragier, P.; Studer, R.; Takamatsu, H.; Tanaka, T.; Takeuchi, M.; Thomaides, H.B.; Vagner, V.; van Dijl, J.M.; Watabe, K.; Wipat, A; Yamamoto, H.; Yamamoto, M.; Yamamoto, Y.; Yamane, K.; Yata, K.; Yoshida, K.; Yoshikawa, H.; Zuber, U.; Ogasawara, N.; Ishio, [No Value

    2003-01-01

    To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among approximate to4,100 genes of the organism, only 192 were shown to be indispensable by this or previous work. Another 79 genes were

  1. Silence of the Genes

    Indian Academy of Sciences (India)

    Srimath

    The human genome codes for ~35,000 genes and all these genes are not expressed in every cell. The time and site of gene expression is very precisely regulated. In any cell, only. Silence of the Genes. 2006 Nobel Prize in Physiology or Medicine. Utpal Nath and Saumitra Das. Keywords. RNA interference, siRNA,.

  2. Human Gene Therapy: Genes without Frontiers?

    Science.gov (United States)

    Simon, Eric J.

    2002-01-01

    Describes the latest advancements and setbacks in human gene therapy to provide reference material for biology teachers to use in their science classes. Focuses on basic concepts such as recombinant DNA technology, and provides examples of human gene therapy such as severe combined immunodeficiency syndrome, familial hypercholesterolemia, and…

  3. Harmful loading of water courses caused by precipitation. Phase 2. Part project 8: Loading of harmful substances by precipitation. Pt. A, B, and C. Pt. A: Harmful substances introduced by atmospheric deposition dust.Pt. B: Sources and mobility of harmful substances. Pt. C: Load of harmful substances from roof and road surfaces; NIEDERschlagsbedingte SCHadstoffbeLAstung der Gewaesser - Phase 2. Teilprojekt 8: Schadstoffbelastung durch Niederschlag. T. A, B und C. T. A: Schadstoffeintrag durch atmosphaerische Staubdeposition. T. B: Quellen und Mobilitaet von Schadstoffen. T. C: Schadstofffrachten von Dach- und Strassenflaechen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Luetzner, K.; Grothkopp, H.; Gebhard, V.; Rennert, S.; Huth, B.

    1997-02-28

    Stadtgebieten ist die Belastung der Oberflaechenabfluesse nicht allein von der Verkehrsdichte abhaengig. Dies gilt hoechstens ueber die Partikelbeladung hinsichtlich einiger Elemente. Das Fahrverhalten, z.B. haeufiges Anfahren und Bremsen, ist hier ebenso von Bedeutung. Weiterhin beeinflussen die Hintergrundbelastung, Abspuelungen von unbefestigten Flaechen sowie weitere Aktivitaeten (z.B. intensive Bautaetigkeit) die Belastung von Nebenstrassen. Im Verlauf der untersuchten Niederschlagsereignisse war nach ca. 6 mm Niederschlag die Auswaschung der bodennahen Luftschicht beendet. Nach einem gefallenen Niederschlag von jeweils 15 mm konnte bei zwei Ereignissen die nahezu vollstaendige Abspuelung der partikulaeren Fracht festgestellt werden. Rund 80% der Schwermetallbelastung (bei Cu nur ca. 50%) des Einzugsgebietes werden durch die Emissionen des Strassenverkehrs verursacht. Kupferdaecher emittieren rund 40% der Cu-Fracht. Die Schwermetallfracht der Dachabfluessen ist ueberwiegend in der fluessigen Phase enthalten (ausser Pb, Fe), dagegen ist die Fracht der Strassenabfluesse zu ueber 90% partikulaer gebunden. Ein Rueckhalt dieser Schwermetallfracht koennte somit ueber den AFS-Rueckhalt geschehen. Entsprechend der durchgefuehrten Berechnung auf Grundlage bekannter Mittelwerte entfernt die Stadtreinigung Dresden gegenwaertig etwa die Haelfte der Fe- und Ni-Gesamtfracht sowie rund 40% der Cu-, Pb- und Zn-Gesamtfracht des Einzugsgebietes. (orig./AJ)

  4. Tumor targeted gene therapy

    International Nuclear Information System (INIS)

    Kang, Joo Hyun

    2006-01-01

    Knowledge of molecular mechanisms governing malignant transformation brings new opportunities for therapeutic intervention against cancer using novel approaches. One of them is gene therapy based on the transfer of genetic material to an organism with the aim of correcting a disease. The application of gene therapy to the cancer treatment had led to the development of new experimental approaches such as suicidal gene therapy, inhibition of oncogenes and restoration of tumor-suppressor genes. Suicidal gene therapy is based on the expression in tumor cells of a gene encoding an enzyme that converts a prodrug into a toxic product. Representative suicidal genes are Herpes simplex virus type 1 thymidine kinase (HSV1-tk) and cytosine deaminase (CD). Especially, physicians and scientists of nuclear medicine field take an interest in suicidal gene therapy because they can monitor the location and magnitude, and duration of expression of HSV1-tk and CD by PET scanner

  5. Essential Bacillus subtilis genes

    DEFF Research Database (Denmark)

    Kobayashi, K.; Ehrlich, S.D.; Albertini, A.

    2003-01-01

    To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among approximate to4,100 genes of the organism, only 192 were shown to be indispensable by this or previous work. Another 79 genes were...... predicted to be essential. The vast majority of essential genes were categorized in relatively few domains of cell metabolism, with about half involved in information processing, one-fifth involved in the synthesis of cell envelope and the determination of cell shape and division, and one-tenth related...... to cell energetics. Only 4% of essential genes encode unknown functions. Most essential genes are present throughout a wide range of Bacteria, and almost 70% can also be found in Archaea and Eucarya. However, essential genes related to cell envelope, shape, division, and respiration tend to be lost from...

  6. Epigenetics: beyond genes

    CSIR Research Space (South Africa)

    Fossey, A

    2009-06-01

    Full Text Available Gene regulatory processes lead to differential gene expression and are referred to as epigenetic phenomena; these are ubiquitous processes in the biological world. These reversible heritable changes concern DNA and RNA, their interactions...

  7. Evolution of gene expression after gene amplification.

    Science.gov (United States)

    Garcia, Nelson; Zhang, Wei; Wu, Yongrui; Messing, Joachim

    2015-04-24

    We took a rather unique approach to investigate the conservation of gene expression of prolamin storage protein genes across two different subfamilies of the Poaceae. We took advantage of oat plants carrying single maize chromosomes in different cultivars, called oat-maize addition (OMA) lines, which permitted us to determine whether regulation of gene expression was conserved between the two species. We found that γ-zeins are expressed in OMA7.06, which carries maize chromosome 7 even in the absence of the trans-acting maize prolamin-box-binding factor (PBF), which regulates their expression. This is likely because oat PBF can substitute for the function of maize PBF as shown in our transient expression data, using a γ-zein promoter fused to green fluorescent protein (GFP). Despite this conservation, the younger, recently amplified prolamin genes in maize, absent in oat, are not expressed in the corresponding OMAs. However, maize can express the oldest prolamin gene, the wheat high-molecular weight glutenin Dx5 gene, even when maize Pbf is knocked down (through PbfRNAi), and/or another maize transcription factor, Opaque-2 (O2) is knocked out (in maize o2 mutant). Therefore, older genes are conserved in their regulation, whereas younger ones diverged during evolution and eventually acquired a new repertoire of suitable transcriptional activators. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  8. MR REPORTER GENES

    OpenAIRE

    Gilad, Assaf A.; Ziv, Keren; McMahon, Michael T.; van Zijl, Peter C.M.; Neeman, Michal; Bulte, Jeff W.M.

    2008-01-01

    Non-invasive molecular imaging of dynamic processes has benefited tremendously from the use of reporter genes. These genes encode for proteins that emit light, bind radiolabeled probes or, as covered in this review, modulate magnetic resonance (MR) contrast. Reporter genes play a pivotal role in monitoring cell trafficking, gene replacement therapy, protein-protein interactions, neuronal plasticity and embryonic development. Several strategies exist for generating MR contrast: enzyme-catalyze...

  9. Radiotechnologies and gene therapy

    International Nuclear Information System (INIS)

    Xia Jinsong

    2001-01-01

    Gene therapy is an exciting frontier in medicine today. Radiologist will make an uniquely contribution to these exciting new technologies at every level by choosing sites for targeting therapy, perfecting and establishing routes of delivery, developing imaging strategies to monitor therapy and assess gene expression, developing radiotherapeutic used of gene therapy

  10. Silence of the Genes

    Indian Academy of Sciences (India)

    Srimath

    tary to the endogenous sense mRNA produced by the normal gene. The antisense strand binds to the sense strand and blocks protein synthesis. This method of gene inhibition was termed antisense technology. The antisense technology soon became a. RNA interference. (RNAi) is a novel mechanism for controlling gene.

  11. Discovering genes underlying QTL

    Energy Technology Data Exchange (ETDEWEB)

    Vanavichit, Apichart [Kasetsart University, Kamphaengsaen, Nakorn Pathom (Thailand)

    2002-02-01

    A map-based approach has allowed scientists to discover few genes at a time. In addition, the reproductive barrier between cultivated rice and wild relatives has prevented us from utilizing the germ plasm by a map-based approach. Most genetic traits important to agriculture or human diseases are manifested as observable, quantitative phenotypes called Quantitative Trait Loci (QTL). In many instances, the complexity of the phenotype/genotype interaction and the general lack of clearly identifiable gene products render the direct molecular cloning approach ineffective, thus additional strategies like genome mapping are required to identify the QTL in question. Genome mapping requires no prior knowledge of the gene function, but utilizes statistical methods to identify the most likely gene location. To completely characterize genes of interest, the initially mapped region of a gene location will have to be narrowed down to a size that is suitable for cloning and sequencing. Strategies for gene identification within the critical region have to be applied after the sequencing of a potentially large clone or set of clones that contains this gene(s). Tremendous success of positional cloning has been shown for cloning many genes responsible for human diseases, including cystic fibrosis and muscular dystrophy as well as plant disease resistance genes. Genome and QTL mapping, positional cloning: the pre-genomics era, comparative approaches to gene identification, and positional cloning: the genomics era are discussed in the report. (M. Suetake)

  12. Discovering genes underlying QTL

    International Nuclear Information System (INIS)

    Vanavichit, Apichart

    2002-01-01

    A map-based approach has allowed scientists to discover few genes at a time. In addition, the reproductive barrier between cultivated rice and wild relatives has prevented us from utilizing the germ plasm by a map-based approach. Most genetic traits important to agriculture or human diseases are manifested as observable, quantitative phenotypes called Quantitative Trait Loci (QTL). In many instances, the complexity of the phenotype/genotype interaction and the general lack of clearly identifiable gene products render the direct molecular cloning approach ineffective, thus additional strategies like genome mapping are required to identify the QTL in question. Genome mapping requires no prior knowledge of the gene function, but utilizes statistical methods to identify the most likely gene location. To completely characterize genes of interest, the initially mapped region of a gene location will have to be narrowed down to a size that is suitable for cloning and sequencing. Strategies for gene identification within the critical region have to be applied after the sequencing of a potentially large clone or set of clones that contains this gene(s). Tremendous success of positional cloning has been shown for cloning many genes responsible for human diseases, including cystic fibrosis and muscular dystrophy as well as plant disease resistance genes. Genome and QTL mapping, positional cloning: the pre-genomics era, comparative approaches to gene identification, and positional cloning: the genomics era are discussed in the report. (M. Suetake)

  13. MR-Imaging optimisation of the articular hip cartilage by using a T{sub 1}-weighted 3-dimensional gradient-echo sequence and the application of a hip joint traction; Magnetresonanztomographische Optimierung der Hueftknorpeldarstellung durch die Wahl einer T{sub 1}-Volumen-Gradienten-Echo-Sequenz und die Anwendung einer Hueftgelenkstraktion

    Energy Technology Data Exchange (ETDEWEB)

    Rosenberg, R. [Heidelberg Univ. (Germany). Orthopaedische Klinik und Poliklinik; Bernd, L. [Heidelberg Univ. (Germany). Orthopaedische Klinik und Poliklinik; Wrazidlo, W. [ATOS-Praxisklinik, Heidelberg (Germany). Radiologische Gemeinschaftspraxis Drs. Lederer, Schneider und Wrazidlo; Lederer, W. [ATOS-Praxisklinik, Heidelberg (Germany). Radiologische Gemeinschaftspraxis Drs. Lederer, Schneider und Wrazidlo; Schneider, S. [ATOS-Praxisklinik, Heidelberg (Germany). Radiologische Gemeinschaftspraxis Drs. Lederer, Schneider und Wrazidlo

    1995-10-01

    Images of three animal cadaver hips, 8 dissected patient femoral heads and 18 hip joints of human corpses, all either with arthrosis stage I-III or artificial cartilage defects, were compared with their corresponding anatomic sections. Additional histomorphologic examinations of the arthrotic cartilages were conducted, and MR-Imaging of 20 healthy and 21 arthrotic patient hips was performed using a specific traction method. Using a T{sub 1}-weighted 3-dimensional gradient-echo sequence and a traction of the hip joint, it was possible due to the low-signal imaging of the joint space to separate in vivo the high-signal femoral head cartilage from the high-signal acetabular cartilage. In horizontal position of the phase-encoding parameter, minimisation of the chemical-shift artifact, mainly in the ventro-lateral areas, was accomplished. MRI measurements of the articular cartilage widths showed significant correlations (p < 0.001) with the corresponding anatomic sections. At the same time the T{sub 1} 3-dimensional gradient-echo sequence of the lateral femoral head with r = 0.94 showed the lowest deviations of the measurements. It was possible with MR-Imaging to distinguish four cartilage qualities. (orig./MG) [Deutsch] Im experimentellen Teil der Studie wurden den MRT-Bildern von drei Kadavertierhueften, 8 resezierten Patientenhueftkoepfen und 18 Leichenhueftgelenken, an denen entweder artifizielle Knorpeldefekte gesetzt wurden oder die ein Koxarthrose-Stadium I-III aufwiesen, die korrespondierenden makroskopischen Kryomikrotomschnitte zugeordnet. Bei den Koxarthrosen erfolgten zusaetzliche histomorphologische Knorpeluntersuchungen. Im klinischen Teil der Studie wurden 20 gesunde und 21 arthrotische Probandenhueftgelenke mit einem speziellen Traktionsverfahren untersucht. Unter Anwendung einer T{sub 1}-Volumen-Gradienten-Echo-Sequenz und einer Traktion am zu untersuchenden Hueftgelenk konnte in vivo durch die signalarme Darstellung des Gelenkspaltes der

  14. CNS metabolism in high-risk drug abuse, German version. Insights gained from {sup 1}H- and {sup 31}P MRS and PET; ZNS-Stoffwechsel bei Missbrauch von Hochrisikodrogen. Erkenntnisse durch {sup 1}H- und {sup 31}P-MRS sowie PET

    Energy Technology Data Exchange (ETDEWEB)

    Bodea, S.V. [Klinik fuer Diagnostische und Interventionelle Neuroradiologie, Universitaetsklinikum des Saarlandes, Homburg/Saar (Germany)

    2017-06-15

    Methamphetamin-, Kokain- und Heroinkonsumenten bis Maerz 2017 wurde ueber PubMed-Recherchen erforscht. Suchbegriffe wie ''methamphetamine'', ''cocaine'', ''heroin'', ''MR spectroscopy'', ''PET'' wurden benutzt. MRT- und PET-Verfahren sind unerlaessliche Werkzeuge bei der Erforschung der zerebralen Reaktionen auf illegale Drogen. Zukuenftige Durchbrueche werden voraussichtlich durch die Untersuchung neuer Neurotransmittersysteme in der PET sowie die Abbildung von Phosphor- und Kohlenstoffmetaboliten in der MRT erfolgen. (orig.)

  15. Contactless detection of the structure and selected parameters of a two-phase-flow by pattern recognition based ultrasonic techniques. Final report; Beruehrungslose Detektion der Struktur und Messung ausgewaehlter Parameter von Zweiphasenstroemungen durch Mustererkennungsverfahren unter Verwendung von Ultraschall als Messsonde. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Prasser, H.M.; Schuetz, P.; Kossok, N.

    1997-11-01

    , wenn die thermodynamischen und geometrischen Randbedingungen nahezu konstant bleiben. Durch die Erweiterung der Trainingsmatrizen um einen, die thermodynamischen Eigenschaften determinierenden Parameter (z.B. Temperatur, Druck) kann das Klassifizierungsverfahren auch auf Faelle mit veraenderlichen Stoffwerten angewandt werden. Das Mustererkennungsverfahren und die Ultraschalltechnik wurden experimentell optimiert und erprobt. Die Ultraschallsignale wurden ausserdem mit Hilfe der Methode des unueberwachten Lernens klassifiziert. Die gefundenen Gruppen von Signalmustern weisen eine deutliche Aehnlichkeit zu bekannten Stroemungskarten auf. Es wird gezeigt, dass das Verfahren des unueberwachten Lernens sich fuer die objektive Aufstellung von Stroemungskarten eignet. In einem speziellen Kapitel werden die Ergebnisse von Tests zur Fuellstandsmessung in einem Segment der Hauptumwaelzleitung von Druckwasserreaktoren vorgestellt. (orig.)

  16. Investigations performed on the compost worm Eisenia fetida and selected species of earthworms concerning the intake of HCB and pyrene with the goal of deriving a bioaccumulation test; Untersuchungen zur Aufnahme von HCB und Pyren durch den Kompostwurm Eisenia fetida und ausgewaehlte Regenwurmwildarten. Ableitung eines Bioakkumulationstests

    Energy Technology Data Exchange (ETDEWEB)

    Vespermann, A.; Riepert, F.; Pflugmacher, J. [Biologische Bundesanstalt fuer Land- und Forstwirtschaft, Inst. fuer Oekotoxikologie im Pflanzenschutz, Berlin (Germany)

    2003-07-01

    . Die Expositionszeit der Regenwuermer betrug 4 Wochen bei woechentlicher Bestimmung der Konzentrationen der Testsubstanzen in den Wuermern und Boeden. Die fuer E. fetida sowie die Wildarten errechneten Anreicherungsfaktoren (AF) beliefen sich bei HCB in Abhaengigkeit des verwendeten Bodens auf Werte zwischen 10 und 17 und bei Pyren auf Werte zwischen 0,9 und 1,7. Durch Umrechnung der Bodenkonzentrationen auf Gehalte in der Wasserphase und Ableitung der entsprechenden Anreicherungsfaktoren kann ein Vergleich mit BCF-Werten, die aus QSAR'S anderer Regenwurmarten und Fischen berechnet wurden, hergestellt werden. Die Ergebnisse lassen den Schluss zu, dass der Regenwurmtest mit E. fetida (OECD 1984, ISO 1998) fuer die Untersuchung der Bioakkumulation geeignet ist. (orig.)

  17. Carboxylesterase 1 genes

    DEFF Research Database (Denmark)

    Rasmussen, Henrik Berg; Madsen, Majbritt Busk

    2018-01-01

    The carboxylesterase 1 gene (CES1) encodes a hydrolase that metabolizes commonly used drugs. The CES1-related pseudogene, carboxylesterase 1 pseudogene 1 (CES1P1), has been implicated in gene exchange with CES1 and in the formation of hybrid genes including the carboxylesterase 1A2 gene (CES1A2...... region was revealed. In conclusion, many procedures for CES1, CES1A2 and CES1P1 genotyping appear to lack specificity. Knowledge about the segmental duplications may improve the typing of these genes....

  18. Gene therapy: An overview

    Directory of Open Access Journals (Sweden)

    Sudip Indu

    2013-01-01

    Full Text Available Gene therapy "the use of genes as medicine" involves the transfer of a therapeutic or working copy of a gene into specific cells of an individual in order to repair a faulty gene copy. The technique may be used to replace a faulty gene, or to introduce a new gene whose function is to cure or to favorably modify the clinical course of a condition. The objective of gene therapy is to introduce new genetic material into target cells while causing no damage to the surrounding healthy cells and tissues, hence the treatment related morbidity is decreased. The delivery system includes a vector that delivers a therapeutic gene into the patient′s target cell. Functional proteins are created from the therapeutic gene causing the cell to return to a normal stage. The vectors used in gene therapy can be viral and non-viral. Gene therapy, an emerging field of biomedicine, is still at infancy and much research remains to be done before this approach to the treatment of condition will realize its full potential.

  19. Human gene essentiality.

    Science.gov (United States)

    Bartha, István; di Iulio, Julia; Venter, J Craig; Telenti, Amalio

    2018-01-01

    A gene can be defined as essential when loss of its function compromises viability of the individual (for example, embryonic lethality) or results in profound loss of fitness. At the population level, identification of essential genes is accomplished by observing intolerance to loss-of-function variants. Several computational methods are available to score gene essentiality, and recent progress has been made in defining essentiality in the non-coding genome. Haploinsufficiency is emerging as a critical aspect of gene essentiality: approximately 3,000 human genes cannot tolerate loss of one of the two alleles. Genes identified as essential in human cell lines or knockout mice may be distinct from those in living humans. Reconciling these discrepancies in how we evaluate gene essentiality has applications in clinical genetics and may offer insights for drug development.

  20. Asymmetrische Alkensynthesen durch Kinetische Racematspaltungen von Aldehyden mit Chiralen Phosphonaten

    DEFF Research Database (Denmark)

    Kreuder, Reinhard; Bodmann, Kerstin; von Zezschwitz, Paultheo

    1998-01-01

    The article gives an overview of how synthetically useful chiral building blocks can be obtained by kinetic resolution of chiral, racemic aldehydes through the reaction with chiral phosphonate reagents....

  1. Die Leben Einsteins eine Reise durch die Geschichte der Physik

    CERN Document Server

    Fiami

    2005-01-01

    Jeder kennt die Namen Einstein, Newton oder Galilei. aber was weiss man über sie? Hier ein Porträt Einsteins anhand von sechs Meilensteinen aus der Geschichte der Physik. Einstein tritt auf als Protagonist in verschiedenen Epochen und bei verschiedenen Entdeckungen, die die Welt verändert haben.

  2. Verletzung der A. subscapularis durch Reposition einer vorderen Schulterluxation

    DEFF Research Database (Denmark)

    Schmal, H; Strohm, P C; Rosahl, S K

    2006-01-01

    report a case with rupture of the arteria subscapularis following reduction of an anterior shoulder dislocation with formation of an axillary hematoma and consecutive paresis of the plexus brachialis. Interdisciplinary operative revision was necessary to remove the hematoma, stop the hemorrhage...

  3. Hartbearbeitungen durch Drehen, Bohren und Fräsen

    Science.gov (United States)

    Barfels, Lutz

    Lange Zeit dachte man bei der spanabhebenden Bearbeitung gehärteter Stähle nur an eine Schleifbearbeitung. Die Entwicklung der hoch- bzw. superharten Schneidstoffe CBN (Kubisches Bornitrid, siehe auch Abschn. 20.3.3.16 "Schneidstoffe" und Abschn. 23.2 "Keramische Werkstoffe") und PKB (Polykristallines kubisches Bornitrid) führte seit den 1980er Jahren zur Zerspanung dieser Werkstoffe mit geometrisch bestimmten Schneiden. Seit dieser Zeit wird die Bearbeitung gehärteter Werkstoffe mit einer Härte über 50 HRC als Hartbearbeitung oder Hartzerspanung bezeichnet. Sie wird sowohl beim Drehen als auch beim Bohren und Fräsen eingesetzt. Die größte Bedeutung hat hier das Hartdrehen. Während das Hartdrehen bis vor einigen Jahren beinah ausschließlich in der Einzel- und Kleinserienfertigung eingesetzt wurde, wird es heute zunehmend auch für Großserien und in der Massenfertigung angewendet. In der Regel bestehen Vorteile für den Einsatz des Hartdrehens dann, wenn in einer Aufspannung mehrere unterschiedliche Geometrie- oder Formelemente (konisch, zylindrisch, …) bearbeitet werden müssen. Bei der Bearbeitung in einer Aufspannung sind sehr gute Lagegenauigkeiten der Funktionsflächen erreichbar. Weiterhin sind Hartdrehprozesse für die Innen- und die Außenkonturbearbeitung von großer Bedeutung.

  4. Bereitstellung von schneller Regelleistung und Momentanreserve durch Batteriespeicher

    OpenAIRE

    Alács, Christian

    2017-01-01

    von Christian Alács Arbeit an der Bibliothek noch nicht eingelangt - Daten nicht geprueft Abweichender Titel nach Übersetzung der Verfasserin/des Verfassers Technische Universität Wien, Univ., Diplomarbeit, 2017

  5. Cost reduction through combined solutions?; Kostensenkung durch Kombinationsloesungen?

    Energy Technology Data Exchange (ETDEWEB)

    Bilitewski, B. [Technische Univ. Dresden (Germany). Inst. fuer Abfallwirtschaft und Altlasten

    1998-09-01

    The implementation of material-specific residue treatment processes requires reconstruction measures both in the case of mechanical-biological and in that of thermal treatment plants. Such measures involves great uncertainties and wide variation in cost prediction. The present paper deals with various factors that influence the costs of residual waste treatment. [Deutsch] Zur Umsetzung der stoffspezifischen Restabfallbehandlung sind Anpassungen sowohl der derzeit betriebenen mechanisch-biologischen als auch der thermischen Anlagen erforderlich, was zu Unsicherheiten und grossen Schwankungsbreiten bei den Kostenprognosen fuehrt. Der Bericht beleuchtet verschiedene Einflussgroessen auf die Kosten der Behandlung des Restabfalls. (orig./SR)

  6. Energy transparency by business intelligence; Energietransparenz durch Business Intelligence

    Energy Technology Data Exchange (ETDEWEB)

    Flieder, Karl [Academia Nova, Schwechat (Austria). Business Process Management and Engineering

    2012-10-15

    The efficiency of energy is one of several key performance parameters, when it comes to the questions how innovative and economical a plant operates compared to its competitors. However, in order to evaluate energy saving potentials in the industry, we need transparency first: A clear picture of the energy consumption based on products and processes. This transparency can be accomplished through business intelligence (BI). This term encompasses analytical processes and tools that can help to transform enterprise data and information into applied knowledge. In this article, we focus on transparency of the energy consumption through a targeted analysis by making use of business intelligence.

  7. Rechtsmissbrauch durch „AGG-Hopping“ / Steffen Krieger

    Index Scriptorium Estoniae

    Krieger, Steffen

    2016-01-01

    Euroopa Kohtu otsusest, mis puudutab töökohale kandideerija staatust eesmärgiga esitada kahju hüvitamise nõudeid kui teda ei valita tööle ja sellise teguviisi käsitlemist õiguse kuritarvitamisena. Vt. ka lk. 699-700

  8. Allergien durch Stiche und Bisse von Insekten und Spinnentieren

    DEFF Research Database (Denmark)

    Blank, S; Schiener, M; Ollert, M.

    2016-01-01

    Allergic reactions due to stings of Hymenoptera species, such as honeybees or yellow jackets, are well known as severe allergies with potentially fatal outcome. Much less common is that also bites of blood-sucking insects (mosquitos, horseflies, flies, bugs, lice and fleas) and arachnids (ticks) ...

  9. Gesellschaftliche Teilhabe Älterer durch Alltagsmobilität

    Directory of Open Access Journals (Sweden)

    Helga Pelizäus-Hoffmeister

    2013-11-01

    Full Text Available Alltagsmobilität – die Mobilität in der näheren Wohnumgebung – gilt in der aktuellen Mobilitätsforschung als ein wesentliches Fundament gesellschaftlicher Teilhabe. Zahlreiche Studien verweisen darauf, dass sie mit zunehmendem Alter sinkt, was dementsprechend mit einem Verlust an gesellschaftlicher Integration und sinkender Lebensqualität assoziiert wird. Dennoch existieren bislang keine Erkenntnisse darüber, wie dieser Prozess auf der Subjektebene konkret gestaltet wird, wie Veränderungen vor dem Hintergrund welcher (räumlichen Wahrnehmungen und Überzeugungen von den Individuen initiiert werden. Hier setzt die dem Beitrag zugrunde liegende Untersuchung an: Das Ziel ist es, auf der Basis einer qualitativ orientierten Untersuchung herauszuarbeiten, wie Veränderungen der Mobilität im Kontext der subjektiven Konstruktionsleistungen und Handlungen der Älteren in einer gegenstandsorientierten Theorie gefasst und erklärt werden können. In einem zweiten Schritt werden prospektiv Thesen darüber entwickelt, in welcher Weise der Einsatz eines technischen Hilfsmittels – eines "Mobilitätsassistenten", der im Rahmen eines vom BMBF (Bundesministerium für Bildung und Forschung geförderten Projekts entwickelt wird – auf die Wahrnehmungen und Überzeugungen der Älteren Einfluss nehmen und darauf aufbauend deren Mobilität fördern könnte. URN: http://nbn-resolving.de/urn:nbn:de:0114-fqs1401113

  10. Do Housekeeping Genes Exist?

    Science.gov (United States)

    Sun, Bingyun

    2015-01-01

    The searching of human housekeeping (HK) genes has been a long quest since the emergence of transcriptomics, and is instrumental for us to understand the structure of genome and the fundamentals of biological processes. The resolved genes are frequently used in evolution studies and as normalization standards in quantitative gene-expression analysis. Within the past 20 years, more than a dozen HK-gene studies have been conducted, yet none of them sampled human tissues completely. We believe an integration of these results will help remove false positive genes owing to the inadequate sampling. Surprisingly, we only find one common gene across 15 examined HK-gene datasets comprising 187 different tissue and cell types. Our subsequent analyses suggest that it might not be appropriate to rigidly define HK genes as expressed in all tissue types that have diverse developmental, physiological, and pathological states. It might be beneficial to use more robustly identified HK functions for filtering criteria, in which the representing genes can be a subset of genome. These genes are not necessarily the same, and perhaps need not to be the same, everywhere in our body. PMID:25970694

  11. Primetime for Learning Genes.

    Science.gov (United States)

    Keifer, Joyce

    2017-02-11

    Learning genes in mature neurons are uniquely suited to respond rapidly to specific environmental stimuli. Expression of individual learning genes, therefore, requires regulatory mechanisms that have the flexibility to respond with transcriptional activation or repression to select appropriate physiological and behavioral responses. Among the mechanisms that equip genes to respond adaptively are bivalent domains. These are specific histone modifications localized to gene promoters that are characteristic of both gene activation and repression, and have been studied primarily for developmental genes in embryonic stem cells. In this review, studies of the epigenetic regulation of learning genes in neurons, particularly the brain-derived neurotrophic factor gene ( BDNF ), by methylation/demethylation and chromatin modifications in the context of learning and memory will be highlighted. Because of the unique function of learning genes in the mature brain, it is proposed that bivalent domains are a characteristic feature of the chromatin landscape surrounding their promoters. This allows them to be "poised" for rapid response to activate or repress gene expression depending on environmental stimuli.

  12. Genes and Social Behavior

    OpenAIRE

    Robinson, Gene E.; Fernald, Russell D.; Clayton, David F.

    2008-01-01

    What specific genes and regulatory sequences contribute to the organization and functioning of brain circuits that support social behavior? How does social experience interact with information in the genome to modulate these brain circuits? Here we address these questions by highlighting progress that has been made in identifying and understanding two key “vectors of influence” that link genes, brain, and social behavior: 1) social information alters gene readout in the brain to influence beh...

  13. History of gene therapy.

    Science.gov (United States)

    Wirth, Thomas; Parker, Nigel; Ylä-Herttuala, Seppo

    2013-08-10

    Two decades after the initial gene therapy trials and more than 1700 approved clinical trials worldwide we not only have gained much new information and knowledge regarding gene therapy in general, but also learned to understand the concern that has persisted in society. Despite the setbacks gene therapy has faced, success stories have increasingly emerged. Examples for these are the positive recommendation for a gene therapy product (Glybera) by the EMA for approval in the European Union and the positive trials for the treatment of ADA deficiency, SCID-X1 and adrenoleukodystrophy. Nevertheless, our knowledge continues to grow and during the course of time more safety data has become available that helps us to develop better gene therapy approaches. Also, with the increased understanding of molecular medicine, we have been able to develop more specific and efficient gene transfer vectors which are now producing clinical results. In this review, we will take a historical view and highlight some of the milestones that had an important impact on the development of gene therapy. We will also discuss briefly the safety and ethical aspects of gene therapy and address some concerns that have been connected with gene therapy as an important therapeutic modality. Copyright © 2013 Elsevier B.V. All rights reserved.

  14. Human housekeeping genes, revisited.

    Science.gov (United States)

    Eisenberg, Eli; Levanon, Erez Y

    2013-10-01

    Housekeeping genes are involved in basic cell maintenance and, therefore, are expected to maintain constant expression levels in all cells and conditions. Identification of these genes facilitates exposure of the underlying cellular infrastructure and increases understanding of various structural genomic features. In addition, housekeeping genes are instrumental for calibration in many biotechnological applications and genomic studies. Advances in our ability to measure RNA expression have resulted in a gradual increase in the number of identified housekeeping genes. Here, we describe housekeeping gene detection in the era of massive parallel sequencing and RNA-seq. We emphasize the importance of expression at a constant level and provide a list of 3804 human genes that are expressed uniformly across a panel of tissues. Several exceptionally uniform genes are singled out for future experimental use, such as RT-PCR control genes. Finally, we discuss both ways in which current technology can meet some of past obstacles encountered, and several as yet unmet challenges. Copyright © 2013 Elsevier Ltd. All rights reserved.

  15. Recombinant gene expression protocols

    National Research Council Canada - National Science Library

    Tuan, Rocky S

    1997-01-01

    .... A fundamental requirement for successful recombinant gene expression is the design of the cloning vector and the choice of the host organism for expression. Recombinant Gene Expression Protocols grows out of the need for a laboratory manual that provides the reader the background and rationale, as well as the practical protocols for the preparation of...

  16. One gene's shattering effects.

    Science.gov (United States)

    Olsen, Kenneth M

    2012-05-29

    A new study shows that three independent mutations in the Sh1 gene, which encodes a YABBY transcription factor, gave rise to the non-shattering seed phenotype in domesticated sorghum. This same gene may have also had a role in the domestication of other cereals, including maize and rice.

  17. Your Genes, Your Choices

    Science.gov (United States)

    Table of Contents Your Genes, Your Choices describes the Human Genome Project, the science behind it, and the ethical, legal, and social issues that are ... Nothing could be further from the truth. Your Genes, Your Choices points out how the progress of ...

  18. Defect size dependent contrast reduction and additional blurring from scattered radiation reduce the quality of radiological images. A comparison of films and digital detectors; Fehlergroessenabhaengige Kontrastreduktion und zusaetzliche Unschaerfe durch Streustrahlung reduzieren die Bildqualitaet radiologischer Abbildungen. Film und digitale Detektoren im Vergleich

    Energy Technology Data Exchange (ETDEWEB)

    Ewert, Uwe; Beckmann, Joerg; Bellon, Carsten; Jaenisch, Gerd-Ruediger; Zscherpel, Uwe; Jechow, Mirko [Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin (Germany); Grosser, Anja [Technische Fachhochschule Wildau (Germany)

    2008-07-01

    wurde eine zusaetzliche Kontrastreduktion, her-vorgerufen durch Streustrahlung, gefunden, wenn der Detektor sich nahe am Objekt befindet (Kontakttechnik). Der Effekt haengt von der Fehlergroesse, dem Abstand zwi-schen Detektor und Objekt und der Zwischenfilterung ab.

  19. Reduzierbarkeit der Abbrecherquote im Humanmedizinstudium durch das Hamburger Auswahlverfahren für Medizinische Studiengänge - Naturwissenschaftsteil (HAM-Nat [Possible reduction of the medical school dropout number by the Hamburg Assessment test for Medicine - part Natural sciences (HAM-Nat

    Directory of Open Access Journals (Sweden)

    Harendza, Sigrid

    2008-05-01

    eines zukünftigen „guten Arztes“ zu entwickeln und zu validieren. Daher beschränkt sich das Auswahlverfahren in Hamburg auf das pragmatische Ziel die Studienabbrecherzahl zu reduzieren, damit möglichst viele Studienanfänger durch gute Lehre zu „guten Ärzten“ ausgebildet werden. Methodik: Zu diesem Zweck wurde der HAM-Nat (Hamburger Auswahlverfahren für Medizinische Studiengänge - Naturwissenschaftsteil mit 52 Multiple-choice Fragen auf Schulniveau über naturwissenschaftliche Kenntnisse und ihre Anwendung entwickelt, zunächst an 6 Schulen in 3 Bundesländern erprobt und dann mit 333 Studienanfängern im Oktober 2006 durchgeführt. Das Testergebnis und die Abiturnote wurden mit dem Studienerfolg in den ersten beiden Semestern korreliert. Als Studienerfolgsparameter wurde die Anzahl der bestandenen Leistungsnachweise verwendet. Ergebnisse: Das HAM-Nat-Ergebnis korreliert nach dem 2. Semester höher mit dem Studienerfolg als die Abiturnote, von der es relativ unabhängig ist. Insbesonders zeigt die Simulation eines Auswahlverfahrens unter Berücksichtigung von HAM-Nat-Ergebnis und Abiturnote eine deutliche Reduktion der Zahl der Studienabbrecher im Vergleich zur jetzigen Auswahl nur nach Abiturnote. Weitere abgefragte mögliche Studienauswahlparameter wie belegte naturwissenschaftliche Kurse oder eine abgeschlossene Berufsausbildung korrelieren nicht mit besserem Studienerfolg. Schlussfolgerung: Der HAM-Nat ist ein wertvolles zusätzliches Auswahlverfahren für Studienbewerber in der Medizin. Aufgrund dieser Ergebnisse hat die Medizinische Fakultät Hamburg beschlossen, den HAM-Nat für die Studienbewerberauswahl zum Wintersemester 2008/2009 einzuführen.

  20. Radionuclide reporter gene imaging

    International Nuclear Information System (INIS)

    Min, Jung Joon

    2004-01-01

    Recent progress in the development of non-invasive imaging technologies continues to strengthen the role of molecular imaging biological research. These tools have been validated recently in variety of research models, and have been shown to provide continuous quantitative monitoring of the location(s), magnitude, and time-variation of gene expression. This article reviews the principles, characteristics, categories and the use of radionuclide reporter gene imaging technologies as they have been used in imaging cell trafficking, imaging gene therapy, imaging endogenous gene expression and imaging molecular interactions. The studies published to date demonstrate that reporter gene imaging technologies will help to accelerate model validation as well as allow for clinical monitoring of human diseases

  1. Third party annotation gene data set of eutherian lysozyme genes

    Directory of Open Access Journals (Sweden)

    Marko Premzl

    2014-12-01

    Full Text Available The eutherian comparative genomic analysis protocol annotated most comprehensive eutherian lysozyme gene data set. Among 209 potential coding sequences, the third party annotation gene data set of eutherian lysozyme genes included 116 complete coding sequences that first described seven major gene clusters. As one new framework of future experiments, the present integrated gene annotations, phylogenetic analysis and protein molecular evolution analysis proposed new classification and nomenclature of eutherian lysozyme genes.

  2. Gene amplification in carcinogenesis

    Directory of Open Access Journals (Sweden)

    Lucimari Bizari

    2006-01-01

    Full Text Available Gene amplification increases the number of genes in a genome and can give rise to karyotype abnormalities called double minutes (DM and homogeneously staining regions (HSR, both of which have been widely observed in human tumors but are also known to play a major role during embryonic development due to the fact that they are responsible for the programmed increase of gene expression. The etiology of gene amplification during carcinogenesis is not yet completely understood but can be considered a result of genetic instability. Gene amplification leads to an increase in protein expression and provides a selective advantage during cell growth. Oncogenes such as CCND1, c-MET, c-MYC, ERBB2, EGFR and MDM2 are amplified in human tumors and can be associated with increased expression of their respective proteins or not. In general, gene amplification is associated with more aggressive tumors, metastases, resistance to chemotherapy and a decrease in the period during which the patient stays free of the disease. This review discusses the major role of gene amplification in the progression of carcinomas, formation of genetic markers and as possible therapeutic targets for the development of drugs for the treatment of some types of tumors.

  3. Genes and hypertension.

    Science.gov (United States)

    Garcia, E A; Newhouse, S; Caulfield, M J; Munroe, P B

    2003-01-01

    The combination of investigation of rare Mendelian forms of hypertension, candidate gene studies, comparative mapping and genome-wide screening in both animal models and man has led to significant progress in determining new mechanisms of blood pressure control. In this review, the newly discovered blood pressure/cardiovascular genes, WNK kinases and angiotensin converting enzyme 2 and the development of a new anti-hypertensive agent PST2238 are discussed. Major genes causing essential hypertension have yet to be discovered, however, there are now over 20 published genome-wide screens for blood pressure controlling genes. Several regions demonstrate suggestive linkage to the trait and there is some overlap of regions between the different studies. It is hoped that new blood pressure genes will ultimately be discovered using this method. Pharmacogenetic studies in hypertension have only been initiated recently, some are described in this paper. Small studies upon single candidate genes, suggest that the contribution of genetics to the inter-individual variation in blood pressure response to anti-hypertensive therapy, is small, approximately 3-5%. Recently micro-arrays with multiple polymorphisms in multiple genes have been used. After accounting for the additive affects of multiple blood pressure loci, an individual's genetic profile appeared to explain up to 50% of the variation in blood pressure response to therapy. Knowledge of the genetic variants that cause hypertension and influence response to anti-hypertensive therapy will ultimately provide a greater understanding of the molecular mechanisms underlying blood pressure control.

  4. Antisense gene silencing

    DEFF Research Database (Denmark)

    Nielsen, Troels T; Nielsen, Jørgen E

    2013-01-01

    Since the first reports that double-stranded RNAs can efficiently silence gene expression in C. elegans, the technology of RNA interference (RNAi) has been intensively exploited as an experimental tool to study gene function. With the subsequent discovery that RNAi could also be applied...... to mammalian cells, the technology of RNAi expanded from being a valuable experimental tool to being an applicable method for gene-specific therapeutic regulation, and much effort has been put into further refinement of the technique. This review will focus on how RNAi has developed over the years and how...

  5. Rice Genomics: Gene discovery

    Indian Academy of Sciences (India)

    There is a need for discovering candidate genes( a lot of them all over the genome indeed ) and the unlimited allelic variation that can productively take over rice metabolism when cellular water content falls below threshold levels.

  6. Gene Expression Omnibus (GEO)

    Data.gov (United States)

    U.S. Department of Health & Human Services — Gene Expression Omnibus is a public functional genomics data repository supporting MIAME-compliant submissions of array- and sequence-based data. Tools are provided...

  7. Radiosensitivity and genes

    International Nuclear Information System (INIS)

    Hu Qiyue; Lun Mingyue

    1995-07-01

    Reported effects of some oncogenes, tumour suppressor genes and DNA repair genes on sensitivity of cells to ionizing radiation are reviewed. The role of oncogenes in cellular response to irradiation is discussed, especially the extensively studied oncogenes such as the ras gene family. For tumour suppressor genes, mainly the p53, which is increasingly implicated as a gene affecting radiosensitivity, is reviewed. It is considered that there is a cell cycle checkpoint determinant which is postulated to be able to arrest the irradiated cells in G 1 phase to allow them to repair damage before they undergo DNA synthesis. So far there are six DNA repair genes which have been cloned in mammalian cells, but only one, XRCC1, appears to be involved in repair of human X-ray damage. XRCC1 can correct high sisterchromatid exchange levels when transferred into EM 9 cells, but its expression seems to have no correlation with radiosensitivity of human neck and head tumour cells. Radiosensitivity is an intricate issue which may involve many factors. A scheme of cellular reactions after exposure to irradiation is proposed to indicate a possible sequence of events initiated by ionizing radiation

  8. Evidence for homosexuality gene

    Energy Technology Data Exchange (ETDEWEB)

    Pool, R.

    1993-07-16

    A genetic analysis of 40 pairs of homosexual brothers has uncovered a region on the X chromosome that appears to contain a gene or genes for homosexuality. When analyzing the pedigrees of homosexual males, the researcheres found evidence that the trait has a higher likelihood of being passed through maternal genes. This led them to search the X chromosome for genes predisposing to homosexuality. The researchers examined the X chromosomes of pairs of homosexual brothers for regions of DNA that most or all had in common. Of the 40 sets of brothers, 33 shared a set of five markers in the q28 region of the long arm of the X chromosome. The linkage has a LOD score of 4.0, which translates into a 99.5% certainty that there is a gene or genes in this area that predispose males to homosexuality. The chief researcher warns, however, that this one site cannot explain all instances of homosexuality, since there were some cases where the trait seemed to be passed paternally. And even among those brothers where there was no evidence that the trait was passed paternally, seven sets of brothers did not share the Xq28 markers. It seems likely that homosexuality arises from a variety of causes.

  9. Gene-Gene and Gene-Environment Interactions in the Etiology of Breast Cancer

    National Research Council Canada - National Science Library

    Adegoke, Olufemi

    2003-01-01

    The objective of this CDA is to evaluate the gene-gene and gene-environment interactions in the etiology of breast cancer in two ongoing case-control studies, the Shanghai Breast Cancer Study (SBCS...

  10. Harnessing gene expression networks to prioritize candidate epileptic encephalopathy genes.

    Science.gov (United States)

    Oliver, Karen L; Lukic, Vesna; Thorne, Natalie P; Berkovic, Samuel F; Scheffer, Ingrid E; Bahlo, Melanie

    2014-01-01

    We apply a novel gene expression network analysis to a cohort of 182 recently reported candidate Epileptic Encephalopathy genes to identify those most likely to be true Epileptic Encephalopathy genes. These candidate genes were identified as having single variants of likely pathogenic significance discovered in a large-scale massively parallel sequencing study. Candidate Epileptic Encephalopathy genes were prioritized according to their co-expression with 29 known Epileptic Encephalopathy genes. We utilized developing brain and adult brain gene expression data from the Allen Human Brain Atlas (AHBA) and compared this to data from Celsius: a large, heterogeneous gene expression data warehouse. We show replicable prioritization results using these three independent gene expression resources, two of which are brain-specific, with small sample size, and the third derived from a heterogeneous collection of tissues with large sample size. Of the nineteen genes that we predicted with the highest likelihood to be true Epileptic Encephalopathy genes, two (GNAO1 and GRIN2B) have recently been independently reported and confirmed. We compare our results to those produced by an established in silico prioritization approach called Endeavour, and finally present gene expression networks for the known and candidate Epileptic Encephalopathy genes. This highlights sub-networks of gene expression, particularly in the network derived from the adult AHBA gene expression dataset. These networks give clues to the likely biological interactions between Epileptic Encephalopathy genes, potentially highlighting underlying mechanisms and avenues for therapeutic targets.

  11. Third party annotation gene data set of eutherian lysozyme genes

    OpenAIRE

    Premzl, Marko

    2014-01-01

    The eutherian comparative genomic analysis protocol annotated most comprehensive eutherian lysozyme gene data set. Among 209 potential coding sequences, the third party annotation gene data set of eutherian lysozyme genes included 116 complete coding sequences that first described seven major gene clusters. As one new framework of future experiments, the present integrated gene annotations, phylogenetic analysis and protein molecular evolution analysis proposed new classification and nomencla...

  12. Gene therapy prospects--intranasal delivery of therapeutic genes.

    Science.gov (United States)

    Podolska, Karolina; Stachurska, Anna; Hajdukiewicz, Karolina; Małecki, Maciej

    2012-01-01

    Gene therapy is recognized to be a novel method for the treatment of various disorders. Gene therapy strategies involve gene manipulation on broad biological processes responsible for the spreading of diseases. Cancer, monogenic diseases, vascular and infectious diseases are the main targets of gene therapy. In order to obtain valuable experimental and clinical results, sufficient gene transfer methods are required. Therapeutic genes can be administered into target tissues via gene carriers commonly defined as vectors. The retroviral, adenoviral and adeno-associated virus based vectors are most frequently used in the clinic. So far, gene preparations may be administered directly into target organs or by intravenous, intramuscular, intratumor or intranasal injections. It is common knowledge that the number of gene therapy clinical trials has rapidly increased. However, some limitations such as transfection efficiency and stable and long-term gene expression are still not resolved. Consequently, great effort is focused on the evaluation of new strategies of gene delivery. There are many expectations associated with intranasal delivery of gene preparations for the treatment of diseases. Intranasal delivery of therapeutic genes is regarded as one of the most promising forms of pulmonary gene therapy research. Gene therapy based on inhalation of gene preparations offers an alternative way for the treatment of patients suffering from such lung diseases as cystic fibrosis, alpha-1-antitrypsin defect, or cancer. Experimental and first clinical trials based on plasmid vectors or recombinant viruses have revealed that gene preparations can effectively deliver therapeutic or marker genes to the cells of the respiratory tract. The noninvasive intranasal delivery of gene preparations or conventional drugs seems to be very encouraging, although basic scientific research still has to continue.

  13. Radionuclide reporter gene imaging for cardiac gene therapy

    International Nuclear Information System (INIS)

    Inubushi, Masayuki; Tamaki, Nagara

    2007-01-01

    In the field of cardiac gene therapy, angiogenic gene therapy has been most extensively investigated. The first clinical trial of cardiac angiogenic gene therapy was reported in 1998, and at the peak, more than 20 clinical trial protocols were under evaluation. However, most trials have ceased owing to the lack of decisive proof of therapeutic effects and the potential risks of viral vectors. In order to further advance cardiac angiogenic gene therapy, remaining open issues need to be resolved: there needs to be improvement of gene transfer methods, regulation of gene expression, development of much safer vectors and optimisation of therapeutic genes. For these purposes, imaging of gene expression in living organisms is of great importance. In radionuclide reporter gene imaging, ''reporter genes'' transferred into cell nuclei encode for a protein that retains a complementary ''reporter probe'' of a positron or single-photon emitter; thus expression of the reporter genes can be imaged with positron emission tomography or single-photon emission computed tomography. Accordingly, in the setting of gene therapy, the location, magnitude and duration of the therapeutic gene co-expression with the reporter genes can be monitored non-invasively. In the near future, gene therapy may evolve into combination therapy with stem/progenitor cell transplantation, so-called cell-based gene therapy or gene-modified cell therapy. Radionuclide reporter gene imaging is now expected to contribute in providing evidence on the usefulness of this novel therapeutic approach, as well as in investigating the molecular mechanisms underlying neovascularisation and safety issues relevant to further progress in conventional gene therapy. (orig.)

  14. [Gene therapy in cardiology].

    Science.gov (United States)

    Jay, David

    2002-01-01

    The modification of genetic material of living cells for therapeutic purposes have been regarded by many as an unrealized promise. However, recent successful achievements in the field have contributed to vanish this perception and have reopened the possibility to use gene therapy as a medical intervention in humans. In the case of cardiovascular diseases, and despite its high prevalence, the number of approved human gene therapy protocols has remained low. This may be due, at least in part, to the availability of effective alternative therapies for some of the most common vasculopathies. However, recent advances in the understanding of the genetic and molecular bases of the cardiovascular system have opened the possibility to introduce gene therapy in the management of a great variety of cardiovascular disorders. The purpose of this communication is to briefly summarize the progress in this area.

  15. Gene decay in archaea

    Directory of Open Access Journals (Sweden)

    M. W. J. van Passel

    2007-01-01

    Full Text Available The gene-dense chromosomes of archaea and bacteria were long thought to be devoid of pseudogenes, but with the massive increase in available genome sequences, whole genome comparisons between closely related species have identified mutations that have rendered numerous genes inactive. Comparative analyses of sequenced archaeal genomes revealed numerous pseudogenes, which can constitute up to 8.6% of the annotated coding sequences in some genomes. The largest proportion of pseudogenes is created by gene truncations, followed by frameshift mutations. Within archaeal genomes, large numbers of pseudogenes contain more than one inactivating mutation, suggesting that pseudogenes are deleted from the genome more slowly in archaea than in bacteria. Although archaea seem to retain pseudogenes longer than do bacteria, most archaeal genomes have unique repertoires of pseudogenes.

  16. Neighboring Genes Show Correlated Evolution in Gene Expression

    Science.gov (United States)

    Ghanbarian, Avazeh T.; Hurst, Laurence D.

    2015-01-01

    When considering the evolution of a gene’s expression profile, we commonly assume that this is unaffected by its genomic neighborhood. This is, however, in contrast to what we know about the lack of autonomy between neighboring genes in gene expression profiles in extant taxa. Indeed, in all eukaryotic genomes genes of similar expression-profile tend to cluster, reflecting chromatin level dynamics. Does it follow that if a gene increases expression in a particular lineage then the genomic neighbors will also increase in their expression or is gene expression evolution autonomous? To address this here we consider evolution of human gene expression since the human-chimp common ancestor, allowing for both variation in estimation of current expression level and error in Bayesian estimation of the ancestral state. We find that in all tissues and both sexes, the change in gene expression of a focal gene on average predicts the change in gene expression of neighbors. The effect is highly pronounced in the immediate vicinity (genes increasing their expression in humans tend to avoid nuclear lamina domains and be enriched for the gene activator 5-hydroxymethylcytosine, we conclude that, most probably owing to chromatin level control of gene expression, a change in gene expression of one gene likely affects the expression evolution of neighbors, what we term expression piggybacking, an analog of hitchhiking. PMID:25743543

  17. Inferring horizontal gene transfer.

    Directory of Open Access Journals (Sweden)

    Matt Ravenhall

    2015-05-01

    Full Text Available Horizontal or Lateral Gene Transfer (HGT or LGT is the transmission of portions of genomic DNA between organisms through a process decoupled from vertical inheritance. In the presence of HGT events, different fragments of the genome are the result of different evolutionary histories. This can therefore complicate the investigations of evolutionary relatedness of lineages and species. Also, as HGT can bring into genomes radically different genotypes from distant lineages, or even new genes bearing new functions, it is a major source of phenotypic innovation and a mechanism of niche adaptation. For example, of particular relevance to human health is the lateral transfer of antibiotic resistance and pathogenicity determinants, leading to the emergence of pathogenic lineages. Computational identification of HGT events relies upon the investigation of sequence composition or evolutionary history of genes. Sequence composition-based ("parametric" methods search for deviations from the genomic average, whereas evolutionary history-based ("phylogenetic" approaches identify genes whose evolutionary history significantly differs from that of the host species. The evaluation and benchmarking of HGT inference methods typically rely upon simulated genomes, for which the true history is known. On real data, different methods tend to infer different HGT events, and as a result it can be difficult to ascertain all but simple and clear-cut HGT events.

  18. Genes in mammalian reproduction

    Energy Technology Data Exchange (ETDEWEB)

    Gwatkin, R.B.L. [ed.

    1996-11-01

    This is an informative book which deals mainly with genomic imprinting, the role of steroid hormones in development, the expression of a variety of genes during development and the link to hereditary diseases. It is an up-to-date review in a field that is quickly changing and provides valuable basic information and current research trends.

  19. Are there anxious genes?

    Science.gov (United States)

    Morris-Rosendahl, Deborah J.

    2002-01-01

    Anxiety comprises many clinical descriptions and phenotypes. A genetic predisposition to anxiety is undoubted; however, the nature and extent of that contribution is still unclear. Methods for the genetic analysis of such complex disorders is briefly reviewed, followed by a discussion of the comorbidity of anxiety with other psychiatric disorders and their possible common genetic etiology. Extensive genetic studies of the serotonin (5-hydroxytryptamine, 5-HT) transporter (5-HTT) gene have revealed how variation in gene expression can be correlated with anxiety phenotypes. Complete genome-wide linkage scans for panic disorder (PD) susceptibility genes have suggested a locus on chromosome arm 7p, and association studies have highlighted many candidate genes. A highly significant association between phobias, panic disorder, and a duplication at chromosomal region 15q24-26 is one of the most exciting findings to date. Emerging molecular genetic technologies and the use of increasingly sophisticated animal models of anxiety provide great promise for the future of the field. PMID:22033820

  20. Silence of the Genes

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 12; Issue 4. Silence of the Genes - 2006 Nobel Prize in Physiology or Medicine. Utpal Nath Saumitra Das. General Article Volume 12 Issue 4 April 2007 pp 6-18. Fulltext. Click here to view fulltext PDF. Permanent link:

  1. Searching for speciation genes

    DEFF Research Database (Denmark)

    Holt, Benjamin George; Côté, Isabelle M; Emerson, Brent C

    2011-01-01

    Closely related species that show clear phenotypic divergence, but without obvious geographic barriers, can provide opportunities to study how diversification can occur when opportunities for allopatric speciation are limited. We examined genetic divergence in the coral reef fish genus Hypoplectr...... evidence for genes that may be associated with colour morphotype in the genus Hypoplectrus....

  2. How Genes Evolve

    Indian Academy of Sciences (India)

    Haemoglobin is a well known respiratory pigment which transports oxygen to various tissues of the body. Tetrameric haemoglo bin (Hb) molecules consist of two .... production of a tetramer that was more effective in oxygen transport and release as compared to the ancestral monomeric form of haemoglobin. Further, gene ...

  3. pyrophosphatase gene in rye

    Indian Academy of Sciences (India)

    photoperiod. Leaves, stems and roots were collected sepa- rately at 6-h intervals from the start (0 h) to the end (72 h) of each experiment. Each treatment was conducted in triplicate. All samples were immediately frozen in liquid nitrogen and were stored at −80. ◦. C until being used for analysis. Isolation of the ScHP1 gene.

  4. Hidden genes in birds

    Czech Academy of Sciences Publication Activity Database

    Hron, Tomáš; Pajer, Petr; Pačes, Jan; Bartůněk, Petr; Elleder, Daniel

    2015-01-01

    Roč. 16, August 18 (2015) ISSN 1465-6906 R&D Projects: GA MŠk(CZ) LK11215; GA MŠk LO1419 Grant - others:GA MŠk(CZ) LM2010005 Institutional support: RVO:68378050 Keywords : REPETITIVE SEQUENCES * G/C stretches * avian genes Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 11.313, year: 2015

  5. What is a Gene?

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 2; Issue 4. What is a Gene? A Question With Variable Answers. S C Lakhotia. General Article Volume 2 Issue 4 April 1997 pp 38-47. Fulltext. Click here to view fulltext PDF. Permanent link: http://www.ias.ac.in/article/fulltext/reso/002/04/0038-0047 ...

  6. What is a Gene?

    Indian Academy of Sciences (India)

    certain supra-genic aspects of biological information that we inherit through the generations and has a profound role in gene activity. Our understanding of the structure and organization of the genetic material has greatly increased in recent years: we have deciphered the total DNA base sequence of genomes of some.

  7. Suicide genes or p53 gene and p53 target genes as targets for cancer gene therapy by ionizing radiation

    International Nuclear Information System (INIS)

    Liu Bing; Chinese Academy of Sciences, Beijing; Zhang Hong

    2005-01-01

    Radiotherapy has some disadvantages due to the severe side-effect on the normal tissues at a curative dose of ionizing radiation (IR). Similarly, as a new developing approach, gene therapy also has some disadvantages, such as lack of specificity for tumors, limited expression of therapeutic gene, potential biological risk. To certain extent, above problems would be solved by the suicide genes or p53 gene and its target genes therapies targeted by ionizing radiation. This strategy not only makes up the disadvantage from radiotherapy or gene therapy alone, but also promotes success rate on the base of lower dose. By present, there have been several vectors measuring up to be reaching clinical trials. This review focused on the development of the cancer gene therapy through suicide genes or p53 and its target genes mediated by IR. (authors)

  8. Genes2FANs: connecting genes through functional association networks

    Science.gov (United States)

    2012-01-01

    Background Protein-protein, cell signaling, metabolic, and transcriptional interaction networks are useful for identifying connections between lists of experimentally identified genes/proteins. However, besides physical or co-expression interactions there are many ways in which pairs of genes, or their protein products, can be associated. By systematically incorporating knowledge on shared properties of genes from diverse sources to build functional association networks (FANs), researchers may be able to identify additional functional interactions between groups of genes that are not readily apparent. Results Genes2FANs is a web based tool and a database that utilizes 14 carefully constructed FANs and a large-scale protein-protein interaction (PPI) network to build subnetworks that connect lists of human and mouse genes. The FANs are created from mammalian gene set libraries where mouse genes are converted to their human orthologs. The tool takes as input a list of human or mouse Entrez gene symbols to produce a subnetwork and a ranked list of intermediate genes that are used to connect the query input list. In addition, users can enter any PubMed search term and then the system automatically converts the returned results to gene lists using GeneRIF. This gene list is then used as input to generate a subnetwork from the user’s PubMed query. As a case study, we applied Genes2FANs to connect disease genes from 90 well-studied disorders. We find an inverse correlation between the counts of links connecting disease genes through PPI and links connecting diseases genes through FANs, separating diseases into two categories. Conclusions Genes2FANs is a useful tool for interpreting the relationships between gene/protein lists in the context of their various functions and networks. Combining functional association interactions with physical PPIs can be useful for revealing new biology and help form hypotheses for further experimentation. Our finding that disease genes in

  9. Industrial scale gene synthesis.

    Science.gov (United States)

    Notka, Frank; Liss, Michael; Wagner, Ralf

    2011-01-01

    The most recent developments in the area of deep DNA sequencing and downstream quantitative and functional analysis are rapidly adding a new dimension to understanding biochemical pathways and metabolic interdependencies. These increasing insights pave the way to designing new strategies that address public needs, including environmental applications and therapeutic inventions, or novel cell factories for sustainable and reconcilable energy or chemicals sources. Adding yet another level is building upon nonnaturally occurring networks and pathways. Recent developments in synthetic biology have created economic and reliable options for designing and synthesizing genes, operons, and eventually complete genomes. Meanwhile, high-throughput design and synthesis of extremely comprehensive DNA sequences have evolved into an enabling technology already indispensable in various life science sectors today. Here, we describe the industrial perspective of modern gene synthesis and its relationship with synthetic biology. Gene synthesis contributed significantly to the emergence of synthetic biology by not only providing the genetic material in high quality and quantity but also enabling its assembly, according to engineering design principles, in a standardized format. Synthetic biology on the other hand, added the need for assembling complex circuits and large complexes, thus fostering the development of appropriate methods and expanding the scope of applications. Synthetic biology has also stimulated interdisciplinary collaboration as well as integration of the broader public by addressing socioeconomic, philosophical, ethical, political, and legal opportunities and concerns. The demand-driven technological achievements of gene synthesis and the implemented processes are exemplified by an industrial setting of large-scale gene synthesis, describing production from order to delivery. Copyright © 2011 Elsevier Inc. All rights reserved.

  10. Genes contributing to prion pathogenesis

    DEFF Research Database (Denmark)

    Tamgüney, Gültekin; Giles, Kurt; Glidden, David V

    2008-01-01

    incubation times, indicating that the conversion reaction may be influenced by other gene products. To identify genes that contribute to prion pathogenesis, we analysed incubation times of prions in mice in which the gene product was inactivated, knocked out or overexpressed. We tested 20 candidate genes...... show that many genes previously implicated in prion replication have no discernible effect on the pathogenesis of prion disease. While most genes tested did not significantly affect survival times, ablation of the amyloid beta (A4) precursor protein (App) or interleukin-1 receptor, type I (Il1r1...

  11. Patenting human genes: Chinese academic articles' portrayal of gene patents.

    Science.gov (United States)

    Du, Li

    2018-04-24

    The patenting of human genes has been the subject of debate for decades. While China has gradually come to play an important role in the global genomics-based testing and treatment market, little is known about Chinese scholars' perspectives on patent protection for human genes. A content analysis of academic literature was conducted to identify Chinese scholars' concerns regarding gene patents, including benefits and risks of patenting human genes, attitudes that researchers hold towards gene patenting, and any legal and policy recommendations offered for the gene patent regime in China. 57.2% of articles were written by law professors, but scholars from health sciences, liberal arts, and ethics also participated in discussions on gene patent issues. While discussions of benefits and risks were relatively balanced in the articles, 63.5% of the articles favored gene patenting in general and, of the articles (n = 41) that explored gene patents in the Chinese context, 90.2% supported patent protections for human genes in China. The patentability of human genes was discussed in 33 articles, and 75.8% of these articles reached the conclusion that human genes are patentable. Chinese scholars view the patent regime as an important legal tool to protect the interests of inventors and inventions as well as the genetic resources of China. As such, many scholars support a gene patent system in China. These attitudes towards gene patents remain unchanged following the court ruling in the Myriad case in 2013, but arguments have been raised about the scope of gene patents, in particular that the increasing numbers of gene patents may negatively impact public health in China.

  12. Gene set analysis for longitudinal gene expression data

    Directory of Open Access Journals (Sweden)

    Piepho Hans-Peter

    2011-07-01

    Full Text Available Abstract Background Gene set analysis (GSA has become a successful tool to interpret gene expression profiles in terms of biological functions, molecular pathways, or genomic locations. GSA performs statistical tests for independent microarray samples at the level of gene sets rather than individual genes. Nowadays, an increasing number of microarray studies are conducted to explore the dynamic changes of gene expression in a variety of species and biological scenarios. In these longitudinal studies, gene expression is repeatedly measured over time such that a GSA needs to take into account the within-gene correlations in addition to possible between-gene correlations. Results We provide a robust nonparametric approach to compare the expressions of longitudinally measured sets of genes under multiple treatments or experimental conditions. The limiting distributions of our statistics are derived when the number of genes goes to infinity while the number of replications can be small. When the number of genes in a gene set is small, we recommend permutation tests based on our nonparametric test statistics to achieve reliable type I error and better power while incorporating unknown correlations between and within-genes. Simulation results demonstrate that the proposed method has a greater power than other methods for various data distributions and heteroscedastic correlation structures. This method was used for an IL-2 stimulation study and significantly altered gene sets were identified. Conclusions The simulation study and the real data application showed that the proposed gene set analysis provides a promising tool for longitudinal microarray analysis. R scripts for simulating longitudinal data and calculating the nonparametric statistics are posted on the North Dakota INBRE website http://ndinbre.org/programs/bioinformatics.php. Raw microarray data is available in Gene Expression Omnibus (National Center for Biotechnology Information with

  13. Optimal Reference Genes for Gene Expression Normalization in Trichomonas vaginalis.

    Science.gov (United States)

    dos Santos, Odelta; de Vargas Rigo, Graziela; Frasson, Amanda Piccoli; Macedo, Alexandre José; Tasca, Tiana

    2015-01-01

    Trichomonas vaginalis is the etiologic agent of trichomonosis, the most common non-viral sexually transmitted disease worldwide. This infection is associated with several health consequences, including cervical and prostate cancers and HIV acquisition. Gene expression analysis has been facilitated because of available genome sequences and large-scale transcriptomes in T. vaginalis, particularly using quantitative real-time polymerase chain reaction (qRT-PCR), one of the most used methods for molecular studies. Reference genes for normalization are crucial to ensure the accuracy of this method. However, to the best of our knowledge, a systematic validation of reference genes has not been performed for T. vaginalis. In this study, the transcripts of nine candidate reference genes were quantified using qRT-PCR under different cultivation conditions, and the stability of these genes was compared using the geNorm and NormFinder algorithms. The most stable reference genes were α-tubulin, actin and DNATopII, and, conversely, the widely used T. vaginalis reference genes GAPDH and β-tubulin were less stable. The PFOR gene was used to validate the reliability of the use of these candidate reference genes. As expected, the PFOR gene was upregulated when the trophozoites were cultivated with ferrous ammonium sulfate when the DNATopII, α-tubulin and actin genes were used as normalizing gene. By contrast, the PFOR gene was downregulated when the GAPDH gene was used as an internal control, leading to misinterpretation of the data. These results provide an important starting point for reference gene selection and gene expression analysis with qRT-PCR studies of T. vaginalis.

  14. Optimal Reference Genes for Gene Expression Normalization in Trichomonas vaginalis.

    Directory of Open Access Journals (Sweden)

    Odelta dos Santos

    Full Text Available Trichomonas vaginalis is the etiologic agent of trichomonosis, the most common non-viral sexually transmitted disease worldwide. This infection is associated with several health consequences, including cervical and prostate cancers and HIV acquisition. Gene expression analysis has been facilitated because of available genome sequences and large-scale transcriptomes in T. vaginalis, particularly using quantitative real-time polymerase chain reaction (qRT-PCR, one of the most used methods for molecular studies. Reference genes for normalization are crucial to ensure the accuracy of this method. However, to the best of our knowledge, a systematic validation of reference genes has not been performed for T. vaginalis. In this study, the transcripts of nine candidate reference genes were quantified using qRT-PCR under different cultivation conditions, and the stability of these genes was compared using the geNorm and NormFinder algorithms. The most stable reference genes were α-tubulin, actin and DNATopII, and, conversely, the widely used T. vaginalis reference genes GAPDH and β-tubulin were less stable. The PFOR gene was used to validate the reliability of the use of these candidate reference genes. As expected, the PFOR gene was upregulated when the trophozoites were cultivated with ferrous ammonium sulfate when the DNATopII, α-tubulin and actin genes were used as normalizing gene. By contrast, the PFOR gene was downregulated when the GAPDH gene was used as an internal control, leading to misinterpretation of the data. These results provide an important starting point for reference gene selection and gene expression analysis with qRT-PCR studies of T. vaginalis.

  15. Gene therapy in keratoconus

    Directory of Open Access Journals (Sweden)

    Mahgol Farjadnia

    2015-01-01

    Full Text Available Keratoconus (KC is the most common ectasia of the cornea and is a common reason for corneal transplant. Therapeutic strategies that can arrest the progression of this disease and modify the underlying pathogenesis are getting more and more popularity among scientists. Cumulating data represent strong evidence of a genetic role in the pathogenesis of KC. Different loci have been identified, and certain mutations have also been mapped for this disease. Moreover, Biophysical properties of the cornea create an appropriate candidate of this tissue for gene therapy. Immune privilege, transparency and ex vivo stability are among these properties. Recent advantage in vectors, besides the ability to modulate the corneal milieu for accepting the target gene for a longer period and fruitful translation, make a big hope for stupendous results reasonable.

  16. Graphene based gene transfection

    Science.gov (United States)

    Feng, Liangzhu; Zhang, Shuai; Liu, Zhuang

    2011-03-01

    Graphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we successfully use graphene as a non-toxic nano-vehicle for efficient gene transfection. Graphene oxide (GO) is bound with cationic polymers, polyethyleneimine (PEI) with two different molecular weights at 1.2 kDa and 10 kDa, forming GO-PEI-1.2k and GO-PEG-10k complexes, respectively, both of which are stable in physiological solutions. Cellular toxicity tests reveal that our GO-PEI-10k complex exhibits significantly reduced toxicity to the treated cells compared to the bare PEI-10k polymer. The positively charged GO-PEI complexes are able to further bind with plasmid DNA (pDNA) for intracellular transfection of the enhanced green fluorescence protein (EGFP) gene in HeLa cells. While EGFP transfection with PEI-1.2k appears to be ineffective, high EGFP expression is observed using the corresponding GO-PEI-1.2k as the transfection agent. On the other hand, GO-PEI-10k shows similar EGFP transfection efficiency but lower toxicity compared with PEI-10k. Our results suggest graphene to be a novel gene delivery nano-vector with low cytotoxicity and high transfection efficiency, promising for future applications in non-viral based gene therapy.Graphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we successfully use graphene as a non-toxic nano-vehicle for efficient gene transfection. Graphene oxide (GO) is bound with cationic polymers, polyethyleneimine (PEI) with two different molecular weights at 1.2 kDa and 10 kDa, forming GO-PEI-1.2k and GO-PEG-10k complexes, respectively, both of which are stable in physiological solutions. Cellular toxicity tests reveal that our GO-PEI-10k complex exhibits significantly reduced toxicity to the treated cells compared to the bare PEI

  17. Gene Expression in Bone

    Science.gov (United States)

    D'Ambrogio, A.

    Skeletal system has two main functions, to provide mechanical integrity for both locomotion and protection and to play an important role in mineral homeostasis. There is extensive evidence showing loss of bone mass during long-term Space-Flights. The loss is due to a break in the equilibrium between the activity of osteoblasts (the cells that forms bone) and the activity of osteoclasts (the cells that resorbs bone). Surprisingly, there is scanty information about the possible altered gene expression occurring in cells that form bone in microgravity.(Just 69 articles result from a "gene expression in microgravity" MedLine query.) Gene-chip or microarray technology allows to screen thousands of genes at the same time: the use of this technology on samples coming from cells exposed to microgravity could provide us with many important informations. For example, the identification of the molecules or structures which are the first sensors of the mechanical stress derived from lack of gravity, could help in understanding which is the first event leading to bone loss due to long-term exposure to microgravity. Consequently, this structure could become a target for a custom-designed drug. It is evident that bone mass loss, observed during long-time stay in Space, represents an accelerated model of what happens in aging osteoporosis. Therefore, the discovery and design of drugs able to interfere with the bone-loss process, could help also in preventing negative physiological processes normally observed on Earth. Considering the aims stated above, my research is designed to:

  18. Gene Porter Bridwell

    Science.gov (United States)

    1994-01-01

    Gene Porter Bridwell served as the director of the Marshall Space Flight Center from January 6, 1994 until February 3, 1996, when he retired from NASA after thirty-four years service. Bridwell, a Marshall employee since 1962, had been Marshall's Space Shuttle Projects Office Director and Space Station Redesign Team deputy manager. Under Bridwell, Marshall worked to develop its role as a Center of Excellence for propulsion and for providing access to space.

  19. Mutant genes in pea breeding

    International Nuclear Information System (INIS)

    Swiecicki, W.K.

    1990-01-01

    Full text: Mutations of genes Dpo (dehiscing pods) and A (anthocyanin synthesis) played a role in pea domestication. A number of other genes were important in cultivar development for 3 types of usage (dry seeds, green vegetable types, fodder), e.g. fn, fna, le, p, v, fas and af. New genes (induced and spontaneous), are important for present ideotypes and are registered by the Pisum Genetics Association (PGA). Comparison of a pea variety ideotype with the variation available in gene banks shows that breeders need 'new' features. In mutation induction experiments, genotype, mutagen and method of treatment (e.g. combined or fractionated doses) are varied for broadening the mutation spectrum and selecting more genes of agronomic value. New genes are genetically analysed. In Poland, some mutant varieties with the gene afila were registered, controlling lodging by a shorter stem and a higher number of internodes. Really non-lodging pea varieties could strongly increase seed yield. But the probability of detecting a major gene for lodging resistance is low. Therefore, mutant genes with smaller influence on plant architecture are sought, to combine their effect by crossing. Promising seem to be the genes rogue, reductus and arthritic as well as a number of mutant genes not yet genetically identified. The gene det for terminal inflorescence - similarly to Vicia faba - changes plant development. Utilisation of assimilates and ripening should be better. Improvement of harvest index should give higher seed yield. A number of genes controlling disease resistance are well known (eg. Fw, Fnw, En, mo and sbm). Important in mass screening of resistance are closely linked gene markers. Pea gene banks collect respective lines, but mutants induced in highly productive cultivars would be better. Inducing gene markers sometimes seems to be easier than transfer by crossing. Mutation induction in pea breeding is probably more important because a high number of monogenic features are

  20. Regulation of eucaryotic gene expression

    Energy Technology Data Exchange (ETDEWEB)

    Brent, R.; Ptashne, M.S

    1989-05-23

    This patent describes a method of regulating the expression of a gene in a eucaryotic cell. The method consists of: providing in the eucaryotic cell, a peptide, derived from or substantially similar to a peptide of a procaryotic cell able to bind to DNA upstream from or within the gene, the amount of the peptide being sufficient to bind to the gene and thereby control expression of the gene.

  1. Genealogy and gene trees.

    Science.gov (United States)

    Rasmuson, Marianne

    2008-02-01

    Heredity can be followed in persons or in genes. Persons can be identified only a few generations back, but simplified models indicate that universal ancestors to all now living persons have occurred in the past. Genetic variability can be characterized as variants of DNA sequences. Data are available only from living persons, but from the pattern of variation gene trees can be inferred by means of coalescence models. The merging of lines backwards in time leads to a MRCA (most recent common ancestor). The time and place of living for this inferred person can give insights in human evolutionary history. Demographic processes are incorporated in the model, but since culture and customs are known to influence demography the models used ought to be tested against available genealogy. The Icelandic data base offers a possibility to do so and points to some discrepancies. Mitochondrial DNA and Y chromosome patterns give a rather consistent view of human evolutionary history during the latest 100 000 years but the earlier epochs of human evolution demand gene trees with longer branches. The results of such studies reveal as yet unsolved problems about the sources of our genome.

  2. Gene-gene and gene-environmental interactions of childhood asthma: a multifactor dimension reduction approach.

    Directory of Open Access Journals (Sweden)

    Ming-Wei Su

    Full Text Available BACKGROUND: The importance of gene-gene and gene-environment interactions on asthma is well documented in literature, but a systematic analysis on the interaction between various genetic and environmental factors is still lacking. METHODOLOGY/PRINCIPAL FINDINGS: We conducted a population-based, case-control study comprised of seventh-grade children from 14 Taiwanese communities. A total of 235 asthmatic cases and 1,310 non-asthmatic controls were selected for DNA collection and genotyping. We examined the gene-gene and gene-environment interactions between 17 single-nucleotide polymorphisms in antioxidative, inflammatory and obesity-related genes, and childhood asthma. Environmental exposures and disease status were obtained from parental questionnaires. The model-free and non-parametrical multifactor dimensionality reduction (MDR method was used for the analysis. A three-way gene-gene interaction was elucidated between the gene coding glutathione S-transferase P (GSTP1, the gene coding interleukin-4 receptor alpha chain (IL4Ra and the gene coding insulin induced gene 2 (INSIG2 on the risk of lifetime asthma. The testing-balanced accuracy on asthma was 57.83% with a cross-validation consistency of 10 out of 10. The interaction of preterm birth and indoor dampness had the highest training-balanced accuracy at 59.09%. Indoor dampness also interacted with many genes, including IL13, beta-2 adrenergic receptor (ADRB2, signal transducer and activator of transcription 6 (STAT6. We also used likelihood ratio tests for interaction and chi-square tests to validate our results and all tests showed statistical significance. CONCLUSIONS/SIGNIFICANCE: The results of this study suggest that GSTP1, INSIG2 and IL4Ra may influence the lifetime asthma susceptibility through gene-gene interactions in schoolchildren. Home dampness combined with each one of the genes STAT6, IL13 and ADRB2 could raise the asthma risk.

  3. Gene therapy of cancer and development of therapeutic target gene

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Chang Min; Kwon, Hee Chung

    1998-04-01

    We applied HSV-tk/GCV strategy to orthotopic rat hepatoma model and showed anticancer effects of hepatoma. The increased expression of Lac Z gene after adenovirus-mediated gene delivery throughout hepatic artery was thought that is increased the possibility of gene therapy for curing hepatoma. With the construction of kGLP-laboratory, it is possible to produce a good quantity and quality of adenovirus in lage-scale production and purification of adenovirus vector. Also, the analysis of hepatoma related genes by PCR-LOH could be used for the diagnosis of patients and the development of therapeutic gene.

  4. Gene therapy of cancer and development of therapeutic target gene

    International Nuclear Information System (INIS)

    Kim, Chang Min; Kwon, Hee Chung

    1998-04-01

    We applied HSV-tk/GCV strategy to orthotopic rat hepatoma model and showed anticancer effects of hepatoma. The increased expression of Lac Z gene after adenovirus-mediated gene delivery throughout hepatic artery was thought that is increased the possibility of gene therapy for curing hepatoma. With the construction of kGLP-laboratory, it is possible to produce a good quantity and quality of adenovirus in lage-scale production and purification of adenovirus vector. Also, the analysis of hepatoma related genes by PCR-LOH could be used for the diagnosis of patients and the development of therapeutic gene

  5. Gene probes: principles and protocols

    National Research Council Canada - National Science Library

    Aquino de Muro, Marilena; Rapley, Ralph

    2002-01-01

    ... of labeled DNA has allowed genes to be mapped to single chromosomes and in many cases to a single chromosome band, promoting significant advance in human genome mapping. Gene Probes: Principles and Protocols presents the principles for gene probe design, labeling, detection, target format, and hybridization conditions together with detailed protocols, accom...

  6. Independent Gene Discovery and Testing

    Science.gov (United States)

    Palsule, Vrushalee; Coric, Dijana; Delancy, Russell; Dunham, Heather; Melancon, Caleb; Thompson, Dennis; Toms, Jamie; White, Ashley; Shultz, Jeffry

    2010-01-01

    A clear understanding of basic gene structure is critical when teaching molecular genetics, the central dogma and the biological sciences. We sought to create a gene-based teaching project to improve students' understanding of gene structure and to integrate this into a research project that can be implemented by instructors at the secondary level…

  7. Compositional gradients in Gramineae genes

    DEFF Research Database (Denmark)

    Wong, Gane Ka-Shu; Wang, Jun; Tao, Lin

    2002-01-01

    In this study, we describe a property of Gramineae genes, and perhaps all monocot genes, that is not observed in eudicot genes. Along the direction of transcription, beginning at the junction of the 5'-UTR and the coding region, there are gradients in GC content, codon usage, and amino-acid usage...

  8. Gene electrotransfer in clinical trials

    DEFF Research Database (Denmark)

    Gehl, Julie

    2014-01-01

    Electroporation is increasingly being used for delivery of chemotherapy to tumors. Likewise, gene delivery by electroporation is rapidly gaining momentum for both vaccination purposes and for delivery of genes coding for other therapeutic molecules, such as chronic diseases or cancer. This chapte...... describes how gene therapy may be performed using electric pulses to enhance uptake and expression.......Electroporation is increasingly being used for delivery of chemotherapy to tumors. Likewise, gene delivery by electroporation is rapidly gaining momentum for both vaccination purposes and for delivery of genes coding for other therapeutic molecules, such as chronic diseases or cancer. This chapter...

  9. The ethics of gene therapy.

    Science.gov (United States)

    Chan, Sarah; Harris, John

    2006-10-01

    Recent developments have progressed in areas of science that pertain to gene therapy and its ethical implications. This review discusses the current state of therapeutic gene technologies, including stem cell therapies and genetic modification, and identifies ethical issues of concern in relation to the science of gene therapy and its application, including the ethics of embryonic stem cell research and therapeutic cloning, the risks associated with gene therapy, and the ethics of clinical research in developing new therapeutic technologies. Additionally, ethical issues relating to genetic modification itself are considered: the significance of the human genome, the distinction between therapy and enhancement, and concerns regarding gene therapy as a eugenic practice.

  10. Exploring new gene integration sites for gene knock-in by gene-trapping strategy.

    Science.gov (United States)

    Nanchi, Isamu; Yoshimura, Yuki; Nakamura, Kazuomi; Masago, Yusaku; Ohbayashi, Tetsuya; Okuda, Tomohiko

    2015-06-01

    The knock-in mouse is a powerful tool for biological research, but the stability of expression of an integrated gene strongly depends on where it is integrated in the mouse genome. At present, there are an insufficient number of loci suitable for gene knock-in, such as the Rosa26 locus. Therefore, in this study, we developed an efficient strategy for identifying genome loci suitable for gene knock-in and characterized the properties of such loci for gene integration. For efficient discovery and characterization, we constructed a new gene-trapping vector that enables monitoring of the expression of both trapped and integrated genes using fluorescence. We successfully obtained fluorescent-positive mouse embryonic stem cell (mESC) clones with the vector. Thorough analysis of the expression of fluorescent proteins in chimera embryos generated with the obtained mESC clones, some of the gene-trapped chimera embryos showed stable and ubiquitous expression of the integrated gene. Furthermore, adult mice derived from one of the gene-trapped mESC clones showed ubiquitous expression of the integrated gene in various tissues without any unusual phenotype. This indicated that the identified locus possesses high potential for foreign gene integration. Our strategy allows for efficient discovery and characterization of mouse genome loci for gene integration.

  11. Radiopharmaceuticals to monitor gene transfer

    International Nuclear Information System (INIS)

    Wiebe, L. I.; Morin, K. W.; Knaus, E. E.

    1997-01-01

    Advances in genetic engineering and molecular biology have opened the door to disease treatment by transferring genes to cells that are responsible for the pathological condition being addressed. These genes can serve to supplement or introduce the function of indigenous genes that are either inadequately expressed or that are congenitally absent in the patient. They can introduce new functions such as drug sensitization to provide a unique therapeutic target. Gene transfer is readily monitored in vitro using a range of histochemical and biochemical tests that are ''built in'' to the therapeutic gene cassette. In vivo, in situ monitoring of the gene transfer and gene expression processes can be achieved with these tests only if biopsy is possible. Scintigraphic imaging can offer unique information on both the extent and location of gene expression, provided that an appropriate reporter gene is included in the therapeutic cassette. This overview includes a brief orientation to gene transfer therapy and is followed by a review of current approaches to gene therapy imaging. The concluding section deals with imaging based on radiolabelled nucleoside substrates for herpes simplex type-1 thymidine kinase, with emphasis on IVFRU, a stable potent and selective HSV-1 TK substrate developed in their laboratories

  12. Gene finding in novel genomes

    Directory of Open Access Journals (Sweden)

    Korf Ian

    2004-05-01

    Full Text Available Abstract Background Computational gene prediction continues to be an important problem, especially for genomes with little experimental data. Results I introduce the SNAP gene finder which has been designed to be easily adaptable to a variety of genomes. In novel genomes without an appropriate gene finder, I demonstrate that employing a foreign gene finder can produce highly inaccurate results, and that the most compatible parameters may not come from the nearest phylogenetic neighbor. I find that foreign gene finders are more usefully employed to bootstrap parameter estimation and that the resulting parameters can be highly accurate. Conclusion Since gene prediction is sensitive to species-specific parameters, every genome needs a dedicated gene finder.

  13. Gene circuit analysis of the terminal gap gene huckebein.

    Directory of Open Access Journals (Sweden)

    Maksat Ashyraliyev

    2009-10-01

    Full Text Available The early embryo of Drosophila melanogaster provides a powerful model system to study the role of genes in pattern formation. The gap gene network constitutes the first zygotic regulatory tier in the hierarchy of the segmentation genes involved in specifying the position of body segments. Here, we use an integrative, systems-level approach to investigate the regulatory effect of the terminal gap gene huckebein (hkb on gap gene expression. We present quantitative expression data for the Hkb protein, which enable us to include hkb in gap gene circuit models. Gap gene circuits are mathematical models of gene networks used as computational tools to extract regulatory information from spatial expression data. This is achieved by fitting the model to gap gene expression patterns, in order to obtain estimates for regulatory parameters which predict a specific network topology. We show how considering variability in the data combined with analysis of parameter determinability significantly improves the biological relevance and consistency of the approach. Our models are in agreement with earlier results, which they extend in two important respects: First, we show that Hkb is involved in the regulation of the posterior hunchback (hb domain, but does not have any other essential function. Specifically, Hkb is required for the anterior shift in the posterior border of this domain, which is now reproduced correctly in our models. Second, gap gene circuits presented here are able to reproduce mutants of terminal gap genes, while previously published models were unable to reproduce any null mutants correctly. As a consequence, our models now capture the expression dynamics of all posterior gap genes and some variational properties of the system correctly. This is an important step towards a better, quantitative understanding of the developmental and evolutionary dynamics of the gap gene network.

  14. Maximum Gene-Support Tree

    Directory of Open Access Journals (Sweden)

    Yunfeng Shan

    2008-01-01

    Full Text Available Genomes and genes diversify during evolution; however, it is unclear to what extent genes still retain the relationship among species. Model species for molecular phylogenetic studies include yeasts and viruses whose genomes were sequenced as well as plants that have the fossil-supported true phylogenetic trees available. In this study, we generated single gene trees of seven yeast species as well as single gene trees of nine baculovirus species using all the orthologous genes among the species compared. Homologous genes among seven known plants were used for validation of the finding. Four algorithms—maximum parsimony (MP, minimum evolution (ME, maximum likelihood (ML, and neighbor-joining (NJ—were used. Trees were reconstructed before and after weighting the DNA and protein sequence lengths among genes. Rarely a gene can always generate the “true tree” by all the four algorithms. However, the most frequent gene tree, termed “maximum gene-support tree” (MGS tree, or WMGS tree for the weighted one, in yeasts, baculoviruses, or plants was consistently found to be the “true tree” among the species. The results provide insights into the overall degree of divergence of orthologous genes of the genomes analyzed and suggest the following: 1 The true tree relationship among the species studied is still maintained by the largest group of orthologous genes; 2 There are usually more orthologous genes with higher similarities between genetically closer species than between genetically more distant ones; and 3 The maximum gene-support tree reflects the phylogenetic relationship among species in comparison.

  15. MUTATIONS IN CALMODULIN GENES

    DEFF Research Database (Denmark)

    2013-01-01

    The present invention relates to an isolated polynucleotide encoding at least a part of calmodulin and an isolated polypeptide comprising at least a part of a calmodulin protein, wherein the polynucleotide and the polypeptide comprise at least one mutation associated with a cardiac disorder. The ...... the binding of calmodulin to ryanodine receptor 2 and use of such compound in a treatment of an individual having a cardiac disorder. The invention further provides a kit that can be used to detect specific mutations in calmodulin encoding genes....

  16. Genes and Disease: Prader-Willi Syndrome

    Science.gov (United States)

    ... MD): National Center for Biotechnology Information (US); 1998-. Genes and Disease [Internet]. Show details National Center for ... 45K) PDF version of this title (3.8M) Gene sequence Genome view see gene locations Entrez Gene ...

  17. Human AZU-1 gene, variants thereof and expressed gene products

    Science.gov (United States)

    Chen, Huei-Mei; Bissell, Mina

    2004-06-22

    A human AZU-1 gene, mutants, variants and fragments thereof. Protein products encoded by the AZU-1 gene and homologs encoded by the variants of AZU-1 gene acting as tumor suppressors or markers of malignancy progression and tumorigenicity reversion. Identification, isolation and characterization of AZU-1 and AZU-2 genes localized to a tumor suppressive locus at chromosome 10q26, highly expressed in nonmalignant and premalignant cells derived from a human breast tumor progression model. A recombinant full length protein sequences encoded by the AZU-1 gene and nucleotide sequences of AZU-1 and AZU-2 genes and variant and fragments thereof. Monoclonal or polyclonal antibodies specific to AZU-1, AZU-2 encoded protein and to AZU-1, or AZU-2 encoded protein homologs.

  18. One gene, many phenotypes

    Directory of Open Access Journals (Sweden)

    Prasun P

    2007-01-01

    Full Text Available "Phenotype" is the visible or quantifiable effect of the expression of a gene, whereas the specific genetic constitution responsible for a phenotype is called "genotype". It was hoped that phenotype could be accurately predicted if the genotype could be characterized. But, the relationship between the genotype and phenotype is not straightforward. Similar genetic lesions can have entirely different phenotypes. In recent years, there has been tremendous progress in the understanding of the genetic basis of diseases. The extent to which it will be possible to relate findings at the DNA level to the clinical phenotype is difficult to delineate on many occasions. The elucidation of mechanisms underlying genotype-phenotype discrepancies is important as it will influence the use of DNA-based tests in the diagnosis, therapy and counseling of individuals affected with genetic disorders. This issue is pertinent to almost every aspect of medical practice and research in this post-genome era. In this article, we have tried to summarize those factors which are responsible for varied manifestations of lesion(s in a single gene.

  19. Reverse engineering transcriptional gene networks.

    Science.gov (United States)

    Belcastro, Vincenzo; di Bernardo, Diego

    2014-01-01

    The aim of this chapter is a step-by-step guide on how to infer gene networks from gene expression profiles. The definition of a gene network is given in Subheading 1, where the different types of networks are discussed. The chapter then guides the readers through a data-gathering process in order to build a compendium of gene expression profiles from a public repository. Gene expression profiles are then discretized and a statistical relationship between genes, called mutual information (MI), is computed. Gene pairs with insignificant MI scores are then discarded by applying one of the described pruning steps. The retained relationships are then used to build up a Boolean adjacency matrix used as input for a clustering algorithm to divide the network into modules (or communities). The gene network can then be used as a hypothesis generator for discovering gene function and analyzing gene signatures. Some case studies are presented, and an online web-tool called Netview is described.

  20. Bayesian assignment of gene ontology terms to gene expression experiments.

    Science.gov (United States)

    Sykacek, P

    2012-09-15

    Gene expression assays allow for genome scale analyses of molecular biological mechanisms. State-of-the-art data analysis provides lists of involved genes, either by calculating significance levels of mRNA abundance or by Bayesian assessments of gene activity. A common problem of such approaches is the difficulty of interpreting the biological implication of the resulting gene lists. This lead to an increased interest in methods for inferring high-level biological information. A common approach for representing high level information is by inferring gene ontology (GO) terms which may be attributed to the expression data experiment. This article proposes a probabilistic model for GO term inference. Modelling assumes that gene annotations to GO terms are available and gene involvement in an experiment is represented by a posterior probabilities over gene-specific indicator variables. Such probability measures result from many Bayesian approaches for expression data analysis. The proposed model combines these indicator probabilities in a probabilistic fashion and provides a probabilistic GO term assignment as a result. Experiments on synthetic and microarray data suggest that advantages of the proposed probabilistic GO term inference over statistical test-based approaches are in particular evident for sparsely annotated GO terms and in situations of large uncertainty about gene activity. Provided that appropriate annotations exist, the proposed approach is easily applied to inferring other high level assignments like pathways. Source code under GPL license is available from the author. peter.sykacek@boku.ac.at.

  1. Bayesian assignment of gene ontology terms to gene expression experiments

    Science.gov (United States)

    Sykacek, P.

    2012-01-01

    Motivation: Gene expression assays allow for genome scale analyses of molecular biological mechanisms. State-of-the-art data analysis provides lists of involved genes, either by calculating significance levels of mRNA abundance or by Bayesian assessments of gene activity. A common problem of such approaches is the difficulty of interpreting the biological implication of the resulting gene lists. This lead to an increased interest in methods for inferring high-level biological information. A common approach for representing high level information is by inferring gene ontology (GO) terms which may be attributed to the expression data experiment. Results: This article proposes a probabilistic model for GO term inference. Modelling assumes that gene annotations to GO terms are available and gene involvement in an experiment is represented by a posterior probabilities over gene-specific indicator variables. Such probability measures result from many Bayesian approaches for expression data analysis. The proposed model combines these indicator probabilities in a probabilistic fashion and provides a probabilistic GO term assignment as a result. Experiments on synthetic and microarray data suggest that advantages of the proposed probabilistic GO term inference over statistical test-based approaches are in particular evident for sparsely annotated GO terms and in situations of large uncertainty about gene activity. Provided that appropriate annotations exist, the proposed approach is easily applied to inferring other high level assignments like pathways. Availability: Source code under GPL license is available from the author. Contact: peter.sykacek@boku.ac.at PMID:22962488

  2. Generalist genes and learning disabilities.

    Science.gov (United States)

    Plomin, Robert; Kovas, Yulia

    2005-07-01

    The authors reviewed recent quantitative genetic research on learning disabilities that led to the conclusion that genetic diagnoses differ from traditional diagnoses in that the effects of relevant genes are largely general rather than specific. This research suggests that most genes associated with common learning disabilities--language impairment, reading disability, and mathematics disability--are generalists in 3 ways. First, genes that affect common learning disabilities are largely the same genes responsible for normal variation in learning abilities. Second, genes that affect any aspect of a learning disability affect other aspects of the disability. Third, genes that affect one learning disability are also likely to affect other learning disabilities. These quantitative genetic findings have far-reaching implications for molecular genetics and neuroscience as well as psychology. Copyright 2005 APA, all rights reserved.

  3. Differential Gene Expression and Aging

    Directory of Open Access Journals (Sweden)

    Laurent Seroude

    2002-01-01

    Full Text Available It has been established that an intricate program of gene expression controls progression through the different stages in development. The equally complex biological phenomenon known as aging is genetically determined and environmentally modulated. This review focuses on the genetic component of aging, with a special emphasis on differential gene expression. At least two genetic pathways regulating organism longevity act by modifying gene expression. Many genes are also subjected to age-dependent transcriptional regulation. Some age-related gene expression changes are prevented by caloric restriction, the most robust intervention that slows down the aging process. Manipulating the expression of some age-regulated genes can extend an organism's life span. Remarkably, the activity of many transcription regulatory elements is linked to physiological age as opposed to chronological age, indicating that orderly and tightly controlled regulatory pathways are active during aging.

  4. Gene Therapy for Cartilage Repair

    Science.gov (United States)

    Madry, Henning; Orth, Patrick; Cucchiarini, Magali

    2011-01-01

    The concept of using gene transfer strategies for cartilage repair originates from the idea of transferring genes encoding therapeutic factors into the repair tissue, resulting in a temporarily and spatially defined delivery of therapeutic molecules to sites of cartilage damage. This review focuses on the potential benefits of using gene therapy approaches for the repair of articular cartilage and meniscal fibrocartilage, including articular cartilage defects resulting from acute trauma, osteochondritis dissecans, osteonecrosis, and osteoarthritis. Possible applications for meniscal repair comprise meniscal lesions, meniscal sutures, and meniscal transplantation. Recent studies in both small and large animal models have demonstrated the applicability of gene-based approaches for cartilage repair. Chondrogenic pathways were stimulated in the repair tissue and in osteoarthritic cartilage using genes for polypeptide growth factors and transcription factors. Although encouraging data have been generated, a successful translation of gene therapy for cartilage repair will require an ongoing combined effort of orthopedic surgeons and of basic scientists. PMID:26069580

  5. Gene Therapy for Fracture Repair

    Science.gov (United States)

    2007-05-01

    the periosteal tissues of healing fractures in small animals , and allow more accurate evaluation of the effects of the fracture therapy (Rundle et...X-ray fluoroscopy (Figure 7). Individual animals receiving the MLV-BMP-2/4 gene therapy by either the percutaneous injection or the intramedullary... animal subjects to understand gene expression in the healing response to bone injury and identify novel genes that might accelerate or delay the

  6. A genetic ensemble approach for gene-gene interaction identification

    Directory of Open Access Journals (Sweden)

    Ho Joshua WK

    2010-10-01

    Full Text Available Abstract Background It has now become clear that gene-gene interactions and gene-environment interactions are ubiquitous and fundamental mechanisms for the development of complex diseases. Though a considerable effort has been put into developing statistical models and algorithmic strategies for identifying such interactions, the accurate identification of those genetic interactions has been proven to be very challenging. Methods In this paper, we propose a new approach for identifying such gene-gene and gene-environment interactions underlying complex diseases. This is a hybrid algorithm and it combines genetic algorithm (GA and an ensemble of classifiers (called genetic ensemble. Using this approach, the original problem of SNP interaction identification is converted into a data mining problem of combinatorial feature selection. By collecting various single nucleotide polymorphisms (SNP subsets as well as environmental factors generated in multiple GA runs, patterns of gene-gene and gene-environment interactions can be extracted using a simple combinatorial ranking method. Also considered in this study is the idea of combining identification results obtained from multiple algorithms. A novel formula based on pairwise double fault is designed to quantify the degree of complementarity. Conclusions Our simulation study demonstrates that the proposed genetic ensemble algorithm has comparable identification power to Multifactor Dimensionality Reduction (MDR and is slightly better than Polymorphism Interaction Analysis (PIA, which are the two most popular methods for gene-gene interaction identification. More importantly, the identification results generated by using our genetic ensemble algorithm are highly complementary to those obtained by PIA and MDR. Experimental results from our simulation studies and real world data application also confirm the effectiveness of the proposed genetic ensemble algorithm, as well as the potential benefits of

  7. Genes, evolution and intelligence.

    Science.gov (United States)

    Bouchard, Thomas J

    2014-11-01

    I argue that the g factor meets the fundamental criteria of a scientific construct more fully than any other conception of intelligence. I briefly discuss the evidence regarding the relationship of brain size to intelligence. A review of a large body of evidence demonstrates that there is a g factor in a wide range of species and that, in the species studied, it relates to brain size and is heritable. These findings suggest that many species have evolved a general-purpose mechanism (a general biological intelligence) for dealing with the environments in which they evolved. In spite of numerous studies with considerable statistical power, we know of very few genes that influence g and the effects are very small. Nevertheless, g appears to be highly polygenic. Given the complexity of the human brain, it is not surprising that that one of its primary faculties-intelligence-is best explained by the near infinitesimal model of quantitative genetics.

  8. Gene therapy for hemophilia

    Science.gov (United States)

    Rogers, Geoffrey L.; Herzog, Roland W.

    2015-01-01

    Hemophilia is an X-linked inherited bleeding disorder consisting of two classifications, hemophilia A and hemophilia B, depending on the underlying mutation. Although the disease is currently treatable with intravenous delivery of replacement recombinant clotting factor, this approach represents a significant cost both monetarily and in terms of quality of life. Gene therapy is an attractive alternative approach to the treatment of hemophilia that would ideally provide life-long correction of clotting activity with a single injection. In this review, we will discuss the multitude of approaches that have been explored for the treatment of both hemophilia A and B, including both in vivo and ex vivo approaches with viral and nonviral delivery vectors. PMID:25553466

  9. Introduction: Cancer Gene Networks.

    Science.gov (United States)

    Clarke, Robert

    2017-01-01

    Constructing, evaluating, and interpreting gene networks generally sits within the broader field of systems biology, which continues to emerge rapidly, particular with respect to its application to understanding the complexity of signaling in the context of cancer biology. For the purposes of this volume, we take a broad definition of systems biology. Considering an organism or disease within an organism as a system, systems biology is the study of the integrated and coordinated interactions of the network(s) of genes, their variants both natural and mutated (e.g., polymorphisms, rearrangements, alternate splicing, mutations), their proteins and isoforms, and the organic and inorganic molecules with which they interact, to execute the biochemical reactions (e.g., as enzymes, substrates, products) that reflect the function of that system. Central to systems biology, and perhaps the only approach that can effectively manage the complexity of such systems, is the building of quantitative multiscale predictive models. The predictions of the models can vary substantially depending on the nature of the model and its inputoutput relationships. For example, a model may predict the outcome of a specific molecular reaction(s), a cellular phenotype (e.g., alive, dead, growth arrest, proliferation, and motility), a change in the respective prevalence of cell or subpopulations, a patient or patient subgroup outcome(s). Such models necessarily require computers. Computational modeling can be thought of as using machine learning and related tools to integrate the very high dimensional data generated from modern, high throughput omics technologies including genomics (next generation sequencing), transcriptomics (gene expression microarrays; RNAseq), metabolomics and proteomics (ultra high performance liquid chromatography, mass spectrometry), and "subomic" technologies to study the kinome, methylome, and others. Mathematical modeling can be thought of as the use of ordinary

  10. Gene expression in colorectal cancer

    DEFF Research Database (Denmark)

    Birkenkamp-Demtroder, Karin; Christensen, Lise Lotte; Olesen, Sanne Harder

    2002-01-01

    Understanding molecular alterations in colorectal cancer (CRC) is needed to define new biomarkers and treatment targets. We used oligonucleotide microarrays to monitor gene expression of about 6,800 known genes and 35,000 expressed sequence tags (ESTs) on five pools (four to six samples in each......' C, and clustered Dukes' D separately. Real-time PCR of 10 known genes and 5 ESTs demonstrated excellent reproducibility of the array-based findings. The most frequently altered genes belonged to functional categories of metabolism (22%), transcription and translation (11%), and cellular processes (9...

  11. Synthetic sustained gene delivery systems.

    Science.gov (United States)

    Agarwal, Ankit; Mallapragada, Surya K

    2008-01-01

    Gene therapy today is hampered by the need of a safe and efficient gene delivery system that can provide a sustained therapeutic effect without cytotoxicity or unwanted immune responses. Bolus gene delivery in solution results in the loss of delivered factors via lymphatic system and may cause undesired effects by the escape of bioactive molecules to distant sites. Controlled gene delivery systems, acting as localized depot of genes, provide an extended sustained release of genes, giving prolonged maintenance of the therapeutic level of encoded proteins. They also limit the DNA degradation in the nuclease rich extra-cellular environment. While attempts have been made to adapt existing controlled drug delivery technologies, more novel approaches are being investigated for controlled gene delivery. DNA encapsulated in nano/micro spheres of polymers have been administered systemically/orally to be taken up by the targeted tissues and provide sustained release once internalized. Alternatively, DNA entrapped in hydrogels or scaffolds have been injected/implanted in tissues/cavities as platforms for gene delivery. The present review examines these different modalities for sustained delivery of viral and non-viral gene-delivery vectors. Design parameters and release mechanisms of different systems made with synthetic or natural polymers are presented along with their prospective applications and opportunities for continuous development.

  12. Gene therapy and reproductive medicine.

    Science.gov (United States)

    Stribley, John M; Rehman, Khurram S; Niu, Hairong; Christman, Gregory M

    2002-04-01

    To review the literature on the principles of gene therapy and its potential application in reproductive medicine. Literature review. Gene therapy involves transfer of genetic material to target cells using a delivery system, or vector. Attention has primarily focused on viral vectors. Significant problems remain to be overcome including low efficacy of gene transfer, the transient expression of some vectors, safety issues with modified adenoviruses and retroviruses, and ethical concerns. If these issues can be resolved, gene therapy will be applicable to an increasing spectrum of single and multiple gene disorders, as the Human Genome Project data are analyzed, and the genetic component of human disease becomes better understood. Gynecologic gene therapy has advanced to human clinical trials for ovarian carcinoma, and shows potential for the treatment of uterine leiomyomata. Obstetric applications of gene therapy, including fetal gene therapy, remain more distant goals. Concerns about the safety of human gene therapy research are being actively addressed, and remarkable progress in improving DNA transfer has been made. The first treatment success for a genetic disease (severe combined immunodeficiency disease) has been achieved, and ongoing research efforts will eventually yield clinical applications in many spheres of reproductive medicine.

  13. Gene- and evidence-based candidate gene selection for schizophrenia and gene feature analysis.

    Science.gov (United States)

    Sun, Jingchun; Han, Leng; Zhao, Zhongming

    2010-01-01

    Schizophrenia is a chronic psychiatric disorder that affects about 1% of the population globally. A tremendous amount of effort has been expended in the past decade, including more than 2400 association studies, to identify genes influencing susceptibility to the disorder. However, few genes or markers have been reliably replicated. The wealth of this information calls for an integration of gene association data, evidence-based gene ranking, and follow-up replication in large sample. The objective of this study is to develop and evaluate evidence-based gene ranking methods and to examine the features of top-ranking candidate genes for schizophrenia. We proposed a gene-based approach for selecting and prioritizing candidate genes by combining odds ratios (ORs) of multiple markers in each association study and then combining ORs in multiple studies of a gene. We named it combination-combination OR method (CCOR). CCOR is similar to our recently published method, which first selects the largest OR of the markers in each study and then combines these ORs in multiple studies (i.e., selection-combination OR method, SCOR), but differs in selecting representative OR in each study. Features of top-ranking genes were examined by Gene Ontology terms and gene expression in tissues. Our evaluation suggested that the SCOR method overall outperforms the CCOR method. Using the SCOR, a list of 75 top-ranking genes was selected for schizophrenia candidate genes (SZGenes). We found that SZGenes had strong correlation with neuro-related functional terms and were highly expressed in brain-related tissues. The scientific landscape for schizophrenia genetics and other complex disease studies is expected to change dramatically in the next a few years, thus, the gene-based combined OR method is useful in candidate gene selection for follow-up association studies and in further artificial intelligence in medicine. This method for prioritization of candidate genes can be applied to other

  14. Gene therapy for meningioma: improved gene delivery with targeted adenoviruses

    NARCIS (Netherlands)

    Dirven, Clemens M. F.; Grill, Jacques; Lamfers, Martine L. M.; van der Valk, Paul; Leonhart, Angelique M.; van Beusechem, Victor W.; Haisma, Hidde J.; Pinedo, Herbert M.; Curiel, David T.; Vandertop, W. Peter; Gerritsen, Winald R.

    2002-01-01

    OBJECT: Due to their surgical inaccessibility or aggressive behavior, some meningiomas cannot be cured with current treatment strategies. Gene therapy is an emerging strategy for the treatment of brain tumors, which the authors investigated to determine whether adenoviruses could be used for gene

  15. Gene therapy for meningioma : improved gene delivery with targeted adenoviruses

    NARCIS (Netherlands)

    Dirven, CMF; Grill, J; Lamfers, MLM; Van der Valk, P; Leonhart, AM; Van Beusechem, VW; Haisma, HJ; Pinedo, HM; Curiel, DT; Vandertop, WP; Gerritsen, WR

    Object. Due to their surgical inaccessibility or aggressive behavior, some meningiomas cannot be cured with current treatment strategies. Gene therapy is an emerging strategy for the treatment of brain tumors, which the authors investigated to determine whether adenoviruses could be used for gene

  16. Evaluation of suitable reference genes for gene expression studies ...

    Indian Academy of Sciences (India)

    2011-12-14

    Dec 14, 2011 ... MADS family of TFs control floral organ identity within each whorl of the flower by activating downstream genes. Measuring gene expression in different tissue types and developmental stages is of fundamental importance in TFs functional research. In last few years, quantitative real-time. PCR (qRT-PCR) ...

  17. Are TMEM genes potential candidate genes for panic disorder?

    DEFF Research Database (Denmark)

    NO, Gregersen; Buttenschøn, Henriette Nørmølle; Hedemand, Anne

    2014-01-01

    We analysed single nucleotide polymorphisms in two transmembrane genes (TMEM98 and TMEM132E) in panic disorder (PD) patients and control individuals from the Faroe Islands, Denmark and Germany. The genes encode single-pass membrane proteins and are located within chromosome 17q11.2-q12...

  18. Classifying genes to the correct Gene Ontology Slim term in Saccharomyces cerevisiae using neighbouring genes with classification learning

    Directory of Open Access Journals (Sweden)

    Tsatsoulis Costas

    2010-05-01

    Full Text Available Abstract Background There is increasing evidence that gene location and surrounding genes influence the functionality of genes in the eukaryotic genome. Knowing the Gene Ontology Slim terms associated with a gene gives us insight into a gene's functionality by informing us how its gene product behaves in a cellular context using three different ontologies: molecular function, biological process, and cellular component. In this study, we analyzed if we could classify a gene in Saccharomyces cerevisiae to its correct Gene Ontology Slim term using information about its location in the genome and information from its nearest-neighbouring genes using classification learning. Results We performed experiments to establish that the MultiBoostAB algorithm using the J48 classifier could correctly classify Gene Ontology Slim terms of a gene given information regarding the gene's location and information from its nearest-neighbouring genes for training. Different neighbourhood sizes were examined to determine how many nearest neighbours should be included around each gene to provide better classification rules. Our results show that by just incorporating neighbour information from each gene's two-nearest neighbours, the percentage of correctly classified genes to their correct Gene Ontology Slim term for each ontology reaches over 80% with high accuracy (reflected in F-measures over 0.80 of the classification rules produced. Conclusions We confirmed that in classifying genes to their correct Gene Ontology Slim term, the inclusion of neighbour information from those genes is beneficial. Knowing the location of a gene and the Gene Ontology Slim information from neighbouring genes gives us insight into that gene's functionality. This benefit is seen by just including information from a gene's two-nearest neighbouring genes.

  19. Gene Synthesis with HG Khorana

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 17; Issue 12. Gene Synthesis with H G Khorana. Marvin H Caruthers. General Article Volume 17 Issue 12 December 2012 pp ... Keywords. Chemical synthesis of genes for yeast alanine tRNA and E. coli supressor tRNA; Khorana's philosophy on science.

  20. Clock genes, ADHD and aggression.

    Science.gov (United States)

    Mogavero, Floriana; Jager, Amanda; Glennon, Jeffrey C

    2016-11-09

    Attention deficit/hyperactivity disorder (ADHD) is frequently associated with comorbid aggression and sleep disturbances. The sleep/wake cycle is under the control of the circadian system which is moderated by clock genes. Clock genes can regulate the transcription of monoamine oxidase A, which is involved in the degradation of monoamines. Disturbances in monoamine interaction with clock genes in those with monoamine gene polymorphisms may regulate susceptibility of ADHD and comorbid aggression/sleep disturbances. While monoamines influence circadian rhythm and clock gene expression, circadian rhythm components modulate aggressive behavior, and altered clock genes expression have been associated with ADHD. We propose a mechanism by which circadian rhythm and clock gene expression may influence ADHD and comorbid aggression through the modulation of neurotransmitters. The role of clock genes in ADHD patients with comorbid aggression awaits further research; therefore we also indicate directions for future studies to help increase understanding of the underlying mechanisms in ADHD with comorbid aggression and sleep disturbances. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. (vitamin B1) biosynthesis genes

    African Journals Online (AJOL)

    In this study, the gene transcripts of first two enzymes in thiamine biosynthesis pathway, THIC and THI1/THI4 were identified and amplified from oil palm tissues. Primers were designed based on sequence comparison of the genes from Arabidopsis thaliana, Zea mays, Oryza sativa and Alnus glutinosa. Oil palm's responses ...

  2. superoxide dismutase gene in sugarcane

    African Journals Online (AJOL)

    Yomi

    2012-01-10

    Jan 10, 2012 ... Superoxide dismutases (SODs) play an important role in stress-tolerance in plants. In this study, for the first time, a full-length cDNA sequence of MnSOD gene, termed as Sc-MnSOD (GenBank accession number: GQ246460), was obtained in sugarcane. Sequence analysis revealed that Sc-MnSOD gene ...

  3. Determining Semantically Related Significant Genes.

    Science.gov (United States)

    Taha, Kamal

    2014-01-01

    GO relation embodies some aspects of existence dependency. If GO term xis existence-dependent on GO term y, the presence of y implies the presence of x. Therefore, the genes annotated with the function of the GO term y are usually functionally and semantically related to the genes annotated with the function of the GO term x. A large number of gene set enrichment analysis methods have been developed in recent years for analyzing gene sets enrichment. However, most of these methods overlook the structural dependencies between GO terms in GO graph by not considering the concept of existence dependency. We propose in this paper a biological search engine called RSGSearch that identifies enriched sets of genes annotated with different functions using the concept of existence dependency. We observe that GO term xcannot be existence-dependent on GO term y, if x- and y- have the same specificity (biological characteristics). After encoding into a numeric format the contributions of GO terms annotating target genes to the semantics of their lowest common ancestors (LCAs), RSGSearch uses microarray experiment to identify the most significant LCA that annotates the result genes. We evaluated RSGSearch experimentally and compared it with five gene set enrichment systems. Results showed marked improvement.

  4. Imaging after vascular gene therapy

    International Nuclear Information System (INIS)

    Manninen, Hannu I.; Yang, Xiaoming

    2005-01-01

    Targets for cardiovascular gene therapy currently include limiting restenosis after balloon angioplasty and stent placement, inhibiting vein bypass graft intimal hyperplasia/stenosis, therapeutic angiogenesis for cardiac and lower-limb ischemia, and prevention of thrombus formation. While catheter angiography is still standard method to follow-up vascular gene transfer, other modern imaging techniques, especially intravascular ultrasound (IVUS), magnetic resonance (MR), and positron emission tomography (PET) imaging provide complementary information about the therapeutic effect of vascular gene transfer in humans. Although molecular imaging of therapeutic gene expression in the vasculatures is still in its technical development phase, it has already offered basic medical science an extremely useful in vivo evaluation tool for non- or minimally invasive imaging of vascular gene therapy

  5. Gene recognition by combination of several gene-finding programs.

    Science.gov (United States)

    Murakami, K; Takagi, T

    1998-01-01

    A number of programs have been developed to predict the eukaryotic gene structures in DNA sequences. However, gene finding is still a challenging problem. We have explored the effectiveness when the results of several gene-finding programs were re-analyzed and combined. We studied several methods with four programs (FEXH, GeneParser3, GEN-SCAN and GRAIL2). By HIGHEST-policy combination method or BOUNDARY method, approximate correlation (AC) improved by 3-5% in comparison with the best single gene-finding program. From another viewpoint, OR-based combination of the four programs is the most reliable to know whether a candidate exon overlaps with the real exon or not, although it is less sensitive than GENSCAN for exon-intron boundaries. Our methods can easily be extended to combine other programs. We have developed a server program (Shirokane System) and a client program (GeneScope) to use the methods. GeneScope is available through a WWW site (http://gf.genome.ad.jp/). (katsu,takagi)@ims.u-tokyo.ac.jp

  6. Targeting Aflatoxin Biosynthetic Genes.

    Science.gov (United States)

    Srour, Ali Y; Fakhoury, Ahmad M; Brown, Robert L

    2017-01-01

    Chemical detoxification and physical destruction of aflatoxins in foods and feed commodities are mostly unattainable in a way that preserves the edibility of the food. Therefore, preventing mycotoxins in general and aflatoxins in particular from entering the food chain is a better approach. This requires early detection of the aflatoxin-causing organisms. Detection and quantification of aflatoxin-producing fungi has always been a challenge, especially within species of Aspergillus and Penicillium. Culture-based methods require a high level of expertise and a list of sophisticated equipment. Furthermore, even for a trained taxonomist, species that are identical in morphology, physiology, and nutritional aspects can be challenging to classify. Fungal taxonomy has changed over the past few decades; more species are being reclassified, and new species are being described due to advances in sequencing and genome assembly. These developments make the use of PCR-based approaches practical, rapid, and more reliable for the identification of fungi to the species level. This chapter presents a variety of protocols to detect and quantify aflatoxin-producing fungi using mycotoxin biosynthesis pathway genes.

  7. Therapeutic genes for anti-HIV/AIDS gene therapy.

    Science.gov (United States)

    Bovolenta, Chiara; Porcellini, Simona; Alberici, Luca

    2013-01-01

    The multiple therapeutic approaches developed so far to cope HIV-1 infection, such as anti-retroviral drugs, germicides and several attempts of therapeutic vaccination have provided significant amelioration in terms of life-quality and survival rate of AIDS patients. Nevertheless, no approach has demonstrated efficacy in eradicating this lethal, if untreated, infection. The curative power of gene therapy has been proven for the treatment of monogenic immunodeficiensies, where permanent gene modification of host cells is sufficient to correct the defect for life-time. No doubt, a similar concept is not applicable for gene therapy of infectious immunodeficiensies as AIDS, where there is not a single gene to be corrected; rather engineered cells must gain immunotherapeutic or antiviral features to grant either short- or long-term efficacy mostly by acquisition of antiviral genes or payloads. Anti-HIV/AIDS gene therapy is one of the most promising strategy, although challenging, to eradicate HIV-1 infection. In fact, genetic modification of hematopoietic stem cells with one or multiple therapeutic genes is expected to originate blood cell progenies resistant to viral infection and thereby able to prevail on infected unprotected cells. Ultimately, protected cells will re-establish a functional immune system able to control HIV-1 replication. More than hundred gene therapy clinical trials against AIDS employing different viral vectors and transgenes have been approved or are currently ongoing worldwide. This review will overview anti-HIV-1 infection gene therapy field evaluating strength and weakness of the transgenes and payloads used in the past and of those potentially exploitable in the future.

  8. Evidence based selection of housekeeping genes

    NARCIS (Netherlands)

    de Jonge, Hendrik J. M.; Fehrmann, Rudolf S. N.; de Bont, Eveline S. J. M.; Hofstra, Robert M. W.; Gerbens, Frans; Kamps, Willem A.; de Vries, Elisabeth G. E.; van der Zee, Ate G. J.; te Meerman, Gerard J.; ter Elst, Arja

    2007-01-01

    For accurate and reliable gene expression analysis, normalization of gene expression data against housekeeping genes (reference or internal control genes) is required. It is known that commonly used housekeeping genes (e. g. ACTB, GAPDH, HPRT1, and B2M) vary considerably under different experimental

  9. Gene Therapy and Children (For Parents)

    Science.gov (United States)

    ... Staying Safe Videos for Educators Search English Español Gene Therapy and Children KidsHealth / For Parents / Gene Therapy and ... caused by a "bad" gene. Two Types of Gene Therapy The two forms of gene therapy are: Somatic ...

  10. Human Lacrimal Gland Gene Expression.

    Directory of Open Access Journals (Sweden)

    Vinay Kumar Aakalu

    Full Text Available The study of human lacrimal gland biology and development is limited. Lacrimal gland tissue is damaged or poorly functional in a number of disease states including dry eye disease. Development of cell based therapies for lacrimal gland diseases requires a better understanding of the gene expression and signaling pathways in lacrimal gland. Differential gene expression analysis between lacrimal gland and other embryologically similar tissues may be helpful in furthering our understanding of lacrimal gland development.We performed global gene expression analysis of human lacrimal gland tissue using Affymetrix ® gene expression arrays. Primary data from our laboratory was compared with datasets available in the NLM GEO database for other surface ectodermal tissues including salivary gland, skin, conjunctiva and corneal epithelium.The analysis revealed statistically significant difference in the gene expression of lacrimal gland tissue compared to other ectodermal tissues. The lacrimal gland specific, cell surface secretory protein encoding genes and critical signaling pathways which distinguish lacrimal gland from other ectodermal tissues are described.Differential gene expression in human lacrimal gland compared with other ectodermal tissue types revealed interesting patterns which may serve as the basis for future studies in directed differentiation among other areas.

  11. Remote control of gene expression.

    Science.gov (United States)

    Long, Xiaochun; Miano, Joseph M

    2007-06-01

    The elucidation of a growing number of species' genomes heralds an unprecedented opportunity to ascertain functional attributes of non-coding sequences. In particular, cis regulatory modules (CRMs) controlling gene expression constitute a rich treasure trove of data to be defined and experimentally validated. Such information will provide insight into cell lineage determination and differentiation and the genetic basis of heritable diseases as well as the development of novel tools for restricting the inactivation of genes to specific cell types or conditions. Historically, the study of CRMs and their individual transcription factor binding sites has been limited to proximal regions around gene loci. Two important by-products of the genomics revolution, artificial chromosome vectors and comparative genomics, have fueled efforts to define an increasing number of CRMs acting remotely to control gene expression. Such regulation from a distance has challenged our perspectives of gene expression control and perhaps the very definition of a gene. This review summarizes current approaches to characterize remote control of gene expression in transgenic mice and inherent limitations for accurately interpreting the essential nature of CRM activity.

  12. Delimiting Coalescence Genes (C-Genes) in Phylogenomic Data Sets.

    Science.gov (United States)

    Springer, Mark S; Gatesy, John

    2018-02-26

    coalescence methods have emerged as a popular alternative for inferring species trees with large genomic datasets, because these methods explicitly account for incomplete lineage sorting. However, statistical consistency of summary coalescence methods is not guaranteed unless several model assumptions are true, including the critical assumption that recombination occurs freely among but not within coalescence genes (c-genes), which are the fundamental units of analysis for these methods. Each c-gene has a single branching history, and large sets of these independent gene histories should be the input for genome-scale coalescence estimates of phylogeny. By contrast, numerous studies have reported the results of coalescence analyses in which complete protein-coding sequences are treated as c-genes even though exons for these loci can span more than a megabase of DNA. Empirical estimates of recombination breakpoints suggest that c-genes may be much shorter, especially when large clades with many species are the focus of analysis. Although this idea has been challenged recently in the literature, the inverse relationship between c-gene size and increased taxon sampling in a dataset-the 'recombination ratchet'-is a fundamental property of c-genes. For taxonomic groups characterized by genes with long intron sequences, complete protein-coding sequences are likely not valid c-genes and are inappropriate units of analysis for summary coalescence methods unless they occur in recombination deserts that are devoid of incomplete lineage sorting (ILS). Finally, it has been argued that coalescence methods are robust when the no-recombination within loci assumption is violated, but recombination must matter at some scale because ILS, a by-product of recombination, is the raison d'etre for coalescence methods. That is, extensive recombination is required to yield the large number of independently segregating c-genes used to infer a species tree. If coalescent methods are powerful

  13. Identification of key player genes in gene regulatory networks.

    Science.gov (United States)

    Nazarieh, Maryam; Wiese, Andreas; Will, Thorsten; Hamed, Mohamed; Helms, Volkhard

    2016-09-06

    Identifying the gene regulatory networks governing the workings and identity of cells is one of the main challenges in understanding processes such as cellular differentiation, reprogramming or cancerogenesis. One particular challenge is to identify the main drivers and master regulatory genes that control such cell fate transitions. In this work, we reformulate this problem as the optimization problems of computing a Minimum Dominating Set and a Minimum Connected Dominating Set for directed graphs. Both MDS and MCDS are applied to the well-studied gene regulatory networks of the model organisms E. coli and S. cerevisiae and to a pluripotency network for mouse embryonic stem cells. The results show that MCDS can capture most of the known key player genes identified so far in the model organisms. Moreover, this method suggests an additional small set of transcription factors as novel key players for governing the cell-specific gene regulatory network which can also be investigated with regard to diseases. To this aim, we investigated the ability of MCDS to define key drivers in breast cancer. The method identified many known drug targets as members of the MDS and MCDS. This paper proposes a new method to identify key player genes in gene regulatory networks. The Java implementation of the heuristic algorithm explained in this paper is available as a Cytoscape plugin at http://apps.cytoscape.org/apps/mcds . The SageMath programs for solving integer linear programming formulations used in the paper are available at https://github.com/maryamNazarieh/KeyRegulatoryGenes and as supplementary material.

  14. Gene therapy of cancer by vaccines carrying inserted immunostimulatory genes

    Czech Academy of Sciences Publication Activity Database

    Bubeník, Jan

    2007-01-01

    Roč. 53, č. 3 (2007), s. 71-73 ISSN 0015-5500 Grant - others:EU-FP6 NoE Clinigene(XE) 018933; Liga proti rakovině, Praha(CZ) XX Institutional research plan: CEZ:AV0Z50520514 Keywords : gene therapy * immunostimulatory genes * vaccine Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 0.596, year: 2007

  15. Cloning and selection of reference genes for gene expression ...

    African Journals Online (AJOL)

    Full length mRNA sequences of Ac-β-actin and Ac-gapdh, and partial mRNA sequences of Ac-18SrRNA and Ac-ubiquitin were cloned from pineapple in this study. The four genes were tested as housekeeping genes in three experimental sets. GeNorm and NormFinder analysis revealed that β-actin was the most ...

  16. Gene expression in colorectal cancer

    DEFF Research Database (Denmark)

    Birkenkamp-Demtroder, Karin; Christensen, Lise Lotte; Olesen, Sanne Harder

    2002-01-01

    Understanding molecular alterations in colorectal cancer (CRC) is needed to define new biomarkers and treatment targets. We used oligonucleotide microarrays to monitor gene expression of about 6,800 known genes and 35,000 expressed sequence tags (ESTs) on five pools (four to six samples in each...... pool) of total RNA from left-sided sporadic colorectal carcinomas. We compared normal tissue to carcinoma tissue from Dukes' stages A-D (noninvasive to distant metastasis) and identified 908 known genes and 4,155 ESTs that changed remarkably from normal to tumor tissue. Based on intensive filtering 226...

  17. Gene Therapy Approaches to Hemoglobinopathies.

    Science.gov (United States)

    Ferrari, Giuliana; Cavazzana, Marina; Mavilio, Fulvio

    2017-10-01

    Gene therapy for hemoglobinopathies is currently based on transplantation of autologous hematopoietic stem cells genetically modified with a lentiviral vector expressing a globin gene under the control of globin transcriptional regulatory elements. Preclinical and early clinical studies showed the safety and potential efficacy of this therapeutic approach as well as the hurdles still limiting its general application. In addition, for both beta-thalassemia and sickle cell disease, an altered bone marrow microenvironment reduces the efficiency of stem cell harvesting as well as engraftment. These hurdles need be addressed for gene therapy for hemoglobinopathies to become a clinical reality. Copyright © 2017 Elsevier Inc. All rights reserved.

  18. The hunt for gene dopers.

    Science.gov (United States)

    Mansour, Mai M H; Azzazy, Hassan M E

    2009-07-01

    Gene doping, the abuse of gene therapy for illicit athletic enhancement, is perceived as a coming threat and is a prime concern to the anti-doping community. This doping technique represents a significant ethical challenge and there are concerns regarding its safety for athletes. This article presents the basics of gene doping, potential strategies for its detection and the role of promising new technologies in aiding detection efforts. These include the use of lab-on-a-chip techniques as well as nanoparticles to enhance the performance of current analytical methods and to develop new doping detection strategies.

  19. Comparing the evolutionary conservation between human essential genes, human orthologs of mouse essential genes and human housekeeping genes.

    Science.gov (United States)

    Lv, Wenhua; Zheng, Jiajia; Luan, Meiwei; Shi, Miao; Zhu, Hongjie; Zhang, Mingming; Lv, Hongchao; Shang, Zhenwei; Duan, Lian; Zhang, Ruijie; Jiang, Yongshuai

    2015-11-01

    Human housekeeping genes are often confused with essential human genes, and several studies regard both types of genes as having the same level of evolutionary conservation. However, this is not necessarily the case. To clarify this, we compared the differences between human housekeeping genes and essential human genes with respect to four aspects: the evolutionary rate (dN/dS), protein sequence identity, single-nucleotide polymorphism (SNP) density and level of linkage disequilibrium (LD). The results showed that housekeeping genes had lower evolutionary rates, higher sequence identities, lower SNP densities and higher levels of LD compared with essential genes. Together, these findings indicate that housekeeping and essential genes are two distinct types of genes, and that housekeeping genes have a higher level of evolutionary conservation. Therefore, we suggest that researchers should pay careful attention to the distinctions between housekeeping genes and essential genes. Moreover, it is still controversial whether we should substitute human orthologs of mouse essential genes for human essential genes. Therefore, we compared the evolutionary features between human orthologs of mouse essential genes and human housekeeping genes and we got inconsistent results in long-term and short-term evolutionary characteristics implying the irrationality of simply replacing human essential genes with human orthologs of mouse essential genes. © The Author 2015. Published by Oxford University Press. For Permissions, please email: journals.permissions@oup.com.

  20. American Society of Gene & Cell Therapy

    Science.gov (United States)

    ... Chicago Learn More Close The American Society of Gene & Cell Therapy ASGCT is the primary membership organization for scientists, ... Therapeutics Official Journal of the American Society of Gene & Cell Therapy Molecular Therapy is the leading journal for gene ...

  1. Basics on Genes and Genetic Disorders

    Science.gov (United States)

    ... for Educators Search English Español The Basics on Genes and Genetic Disorders KidsHealth / For Teens / The Basics ... such as treating health problems. What Is a Gene? To understand how genes work, let's review some ...

  2. Information-processing genes

    International Nuclear Information System (INIS)

    Tahir Shah, K.

    1995-01-01

    There are an estimated 100,000 genes in the human genome of which 97% is non-coding. On the other hand, bacteria have little or no non-coding DNA. Non-coding region includes introns, ALU sequences, satellite DNA, and other segments not expressed as proteins. Why it exists? Why nature has kept non-coding during the long evolutionary period if it has no role in the development of complex life forms? Does complexity of a species somehow correlated to the existence of apparently useless sequences? What kind of capability is encoded within such nucleotide sequences that is a necessary, but not a sufficient condition for the evolution of complex life forms, keeping in mind the C-value paradox and the omnipresence of non-coding segments in higher eurkaryotes and also in many archea and prokaryotes. The physico-chemical description of biological processes is hardware oriented and does not highlight algorithmic or information processing aspect. However, an algorithm without its hardware implementation is useless as much as hardware without its capability to run an algorithm. The nature and type of computation an information-processing hardware can perform depends only on its algorithm and the architecture that reflects the algorithm. Given that enormously difficult tasks such as high fidelity replication, transcription, editing and regulation are all achieved within a long linear sequence, it is natural to think that some parts of a genome are involved is these tasks. If some complex algorithms are encoded with these parts, then it is natural to think that non-coding regions contain processing-information algorithms. A comparison between well-known automatic sequences and sequences constructed out of motifs is found in all species proves the point: noncoding regions are a sort of ''hardwired'' programs, i.e., they are linear representations of information-processing machines. Thus in our model, a noncoding region, e.g., an intron contains a program (or equivalently, it is

  3. Gene discovery in Triatoma infestans

    Directory of Open Access Journals (Sweden)

    de Burgos Nelia

    2011-03-01

    Full Text Available Abstract Background Triatoma infestans is the most relevant vector of Chagas disease in the southern cone of South America. Since its genome has not yet been studied, sequencing of Expressed Sequence Tags (ESTs is one of the most powerful tools for efficiently identifying large numbers of expressed genes in this insect vector. Results In this work, we generated 826 ESTs, resulting in an increase of 47% in the number of ESTs available for T. infestans. These ESTs were assembled in 471 unique sequences, 151 of which represent 136 new genes for the Reduviidae family. Conclusions Among the putative new genes for the Reduviidae family, we identified and described an interesting subset of genes involved in development and reproduction, which constitute potential targets for insecticide development.

  4. Gene Therapy for Parkinson's Disease

    Directory of Open Access Journals (Sweden)

    Rachel Denyer

    2012-01-01

    Full Text Available Current pharmacological and surgical treatments for Parkinson's disease offer symptomatic improvements to those suffering from this incurable degenerative neurological disorder, but none of these has convincingly shown effects on disease progression. Novel approaches based on gene therapy have several potential advantages over conventional treatment modalities. These could be used to provide more consistent dopamine supplementation, potentially providing superior symptomatic relief with fewer side effects. More radically, gene therapy could be used to correct the imbalances in basal ganglia circuitry associated with the symptoms of Parkinson's disease, or to preserve or restore dopaminergic neurons lost during the disease process itself. The latter neuroprotective approach is the most exciting, as it could theoretically be disease modifying rather than simply symptom alleviating. Gene therapy agents using these approaches are currently making the transition from the laboratory to the bedside. This paper summarises the theoretical approaches to gene therapy for Parkinson's disease and the findings of clinical trials in this rapidly changing field.

  5. MHC Genes and Breast Cancer

    National Research Council Canada - National Science Library

    Pillai, Shiv

    2000-01-01

    Tumors are believed to emerge only when immune surveillance fails. We wished to ascertain whether the failure to inherit putative protective alleles of HLA class II genes is linked to the development of breast cancer...

  6. Genes That Influence Blood Pressure

    Science.gov (United States)

    ... Matters September 26, 2011 Genes that Influence Blood Pressure In one of the largest genomic studies ever, ... consortium identified 29 genetic variations that influence blood pressure. More than half of these variants were previously ...

  7. Gene therapy of thyroid carcinoma

    International Nuclear Information System (INIS)

    Zheng Wei; Tan Jian

    2007-01-01

    Normally, differentiated thyroid carcinoma(DTC) is a disease of good prognosis, but about 30% of the tumors are dedifferentiate, which are inaccessible to standard therapeutic procedures such as 'operation, 131 I therapy and thyroid hormone'. Both internal and abroad experts are researching a new therapy of dedifferentiated thyroid carcinoma--gene therapy. Many of them utilize methods of it, but follow different strategies: (1) transduction of the thyroid sodium/iodide transporter gene to make tissues that do not accumulate iodide treatable by 131 I therapy; (2) strengthening of the anti-tumor immune response; (3) suicide gene therapy; (4) depression the generation of tumor cells; (5) gene therapy of anti- vascularization. (authors)

  8. Novel genes in LDL metabolism

    DEFF Research Database (Denmark)

    Christoffersen, Mette; Tybjærg-Hansen, Anne

    2015-01-01

    PURPOSE OF REVIEW: To summarize recent findings from genome-wide association studies (GWAS), whole-exome sequencing of patients with familial hypercholesterolemia and 'exome chip' studies pointing to novel genes in LDL metabolism. RECENT FINDINGS: The genetic loci for ATP-binding cassette......-exome sequencing and 'exome chip' studies have additionally suggested several novel genes in LDL metabolism including insulin-induced gene 2, signal transducing adaptor family member 1, lysosomal acid lipase A, patatin-like phospholipase domain-containing protein 5 and transmembrane 6 superfamily member 2. Most...... of these findings still require independent replications and/or functional studies to confirm the exact role in LDL metabolism and the clinical implications for human health. SUMMARY: GWAS, exome sequencing studies, and recently 'exome chip' studies have suggested several novel genes with effects on LDL cholesterol...

  9. Deregulated genes in sporadic vestibular schwannomas

    DEFF Research Database (Denmark)

    Cayé-Thomasen, Per; Helweg-Larsen, Rehannah Holga Andrea; Stangerup, Sven-Eric

    2010-01-01

    In search of genes associated with vestibular schwannoma tumorigenesis, this study examines the gene expression in human vestibular nerve versus vestibular schwannoma tissue samples using microarray technology....

  10. Endocrine aspects of cancer gene therapy.

    Science.gov (United States)

    Barzon, Luisa; Boscaro, Marco; Palù, Giorgio

    2004-02-01

    The field of cancer gene therapy is in continuous expansion, and technology is quickly moving ahead as far as gene targeting and regulation of gene expression are concerned. This review focuses on the endocrine aspects of gene therapy, including the possibility to exploit hormone and hormone receptor functions for regulating therapeutic gene expression, the use of endocrine-specific genes as new therapeutic tools, the effects of viral vector delivery and transgene expression on the endocrine system, and the endocrine response to viral vector delivery. Present ethical concerns of gene therapy and the risk of germ cell transduction are also discussed, along with potential lines of innovation to improve cell and gene targeting.

  11. MADS-box gene evolution - structure and transcription patterns

    DEFF Research Database (Denmark)

    Johansen, Bo; Pedersen, Louise Buchholt; Skipper, Martin

    2002-01-01

    Mads-box genes, ABC model, Evolution, Phylogeny, Transcription patterns, Gene structure, Conserved motifs......Mads-box genes, ABC model, Evolution, Phylogeny, Transcription patterns, Gene structure, Conserved motifs...

  12. Gene mutations in hepatocellular adenomas

    DEFF Research Database (Denmark)

    Raft, Marie B; Jørgensen, Ernö N; Vainer, Ben

    2015-01-01

    is associated with bi-allelic mutations in the TCF1 gene and morphologically has marked steatosis. β-catenin activating HCA has increased activity of the Wnt/β-catenin pathway and is associated with possible malignant transformation. Inflammatory HCA is characterized by an oncogene-induced inflammation due....... This review offers an overview of the reported gene mutations associated with hepatocellular adenomas together with a discussion of the diagnostic and prognostic value....

  13. Rice Multi-Gene Analysis

    Indian Academy of Sciences (India)

    gdyang

    Maps of all the intronic MIR genes analyzed using MPSS database in rice. Click here for a legend that explains the icons and colors in the image below. Click here to jump in the page below to the specific gene. osa-MIR159f osa-MIR399i osa-MIR418 osa-MIR437 osa-MIR439b osa-MIR439j osa-MIR440 osa-MIR442.

  14. Transgenic Arabidopsis Gene Expression System

    Science.gov (United States)

    Ferl, Robert; Paul, Anna-Lisa

    2009-01-01

    The Transgenic Arabidopsis Gene Expression System (TAGES) investigation is one in a pair of investigations that use the Advanced Biological Research System (ABRS) facility. TAGES uses Arabidopsis thaliana, thale cress, with sensor promoter-reporter gene constructs that render the plants as biomonitors (an organism used to determine the quality of the surrounding environment) of their environment using real-time nondestructive Green Fluorescent Protein (GFP) imagery and traditional postflight analyses.

  15. Gene Therapy in Cardiac Arrhythmias

    OpenAIRE

    Praveen, S.V; Francis, Johnson; Venugopal, K

    2006-01-01

    Gene therapy has progressed from a dream to a bedside reality in quite a few human diseases. From its first application in adenosine deaminase deficiency, through the years, its application has evolved to vascular angiogenesis and cardiac arrhythmias. Gene based biological pacemakers using viral vectors or mesenchymal cells tested in animal models hold much promise. Induction of pacemaker activity within the left bundle branch can provide stable heart rates. Genetic modification of the AV...

  16. Immunoglobulin genes of the turtles.

    Science.gov (United States)

    Magadán-Mompó, Susana; Sánchez-Espinel, Christian; Gambón-Deza, Francisco

    2013-03-01

    The availability of reptile genomes for the use of the scientific community is an exceptional opportunity to study the evolution of immunoglobulin genes. The genome of Chrysemys picta bellii and Pelodiscus sinensis is the first one that has been reported for turtles. The scanning for immunoglobulin genes resulted in the presence of a complex locus for the immunoglobulin heavy chain (IGH). This IGH locus in both turtles contains genes for 13 isotypes in C. picta bellii and 17 in P. sinensis. These correspond with one immunoglobulin M, one immunoglobulin D, several immunoglobulins Y (six in C. picta bellii and eight in P. sinensis), and several immunoglobulins that are similar to immunoglobulin D2 (five in C. picta belli and seven in P. sinensis) that was previously described in Eublepharis macularius. It is worthy to note that IGHD2 are placed in an inverted transcriptional orientation and present sequences for two immunoglobulin domains that are similar to bird IgA domains. Furthermore, its phylogenetic analysis allows us to consider about the presence of IGHA gene in a primitive reptile, so we would be dealing with the memory of the gene that originated from the bird IGHA. In summary, we provide a clear picture of the immunoglobulins present in a turtle, whose analysis supports the idea that turtles emerged from the evolutionary line from the differentiation of birds and the presence of the IGHA gene present in a common ancestor.

  17. Cationic Bolaamphiphiles for Gene Delivery

    Science.gov (United States)

    Tan, Amelia Li Min; Lim, Alisa Xue Ling; Zhu, Yiting; Yang, Yi Yan; Khan, Majad

    2014-05-01

    Advances in medical research have shed light on the genetic cause of many human diseases. Gene therapy is a promising approach which can be used to deliver therapeutic genes to treat genetic diseases at its most fundamental level. In general, nonviral vectors are preferred due to reduced risk of immune response, but they are also commonly associated with low transfection efficiency and high cytotoxicity. In contrast to viral vectors, nonviral vectors do not have a natural mechanism to overcome extra- and intracellular barriers when delivering the therapeutic gene into cell. Hence, its design has been increasingly complex to meet challenges faced in targeting of, penetration of and expression in a specific host cell in achieving more satisfactory transfection efficiency. Flexibility in design of the vector is desirable, to enable a careful and controlled manipulation of its properties and functions. This can be met by the use of bolaamphiphile, a special class of lipid. Unlike conventional lipids, bolaamphiphiles can form asymmetric complexes with the therapeutic gene. The advantage of having an asymmetric complex lies in the different purposes served by the interior and exterior of the complex. More effective gene encapsulation within the interior of the complex can be achieved without triggering greater aggregation of serum proteins with the exterior, potentially overcoming one of the great hurdles faced by conventional single-head cationic lipids. In this review, we will look into the physiochemical considerations as well as the biological aspects of a bolaamphiphile-based gene delivery system.

  18. Cloning human DNA repair genes

    International Nuclear Information System (INIS)

    Jeggo, P.A.; Carr, A.M.; Lehmann, A.R.

    1994-01-01

    Many human genes involved in the repair of UV damage have been cloned using different procedures and they have been of great value in assisting the understanding of the mechanism of nucleotide excision-repair. Genes involved in repair of ionizing radiation damage have proved more difficult to isolate. Positional cloning has localized the XRCC5 gene to a small region of chromosome 2q33-35, and a series of yeast artificial chromosomes covering this region have been isolated. Very recent work has shown that the XRCC5 gene encodes the 80 kDa subunit of the Ku DNA-binding protein. The Ku80 gene also maps to this region. Studies with fission yeast have shown that radiation sensitivity can result not only from defective DNA repair but also from abnormal cell cycle control following DNA damage. Several genes involved in this 'check-point' control in fission yeast have been isolated and characterized in detail. It is likely that a similar checkpoint control mechanism exists in human cells. (author)

  19. Homology-dependent Gene Silencing in Paramecium

    Science.gov (United States)

    Ruiz, Françoise; Vayssié, Laurence; Klotz, Catherine; Sperling, Linda; Madeddu, Luisa

    1998-01-01

    Microinjection at high copy number of plasmids containing only the coding region of a gene into the Paramecium somatic macronucleus led to a marked reduction in the expression of the corresponding endogenous gene(s). The silencing effect, which is stably maintained throughout vegetative growth, has been observed for all Paramecium genes examined so far: a single-copy gene (ND7), as well as members of multigene families (centrin genes and trichocyst matrix protein genes) in which all closely related paralogous genes appeared to be affected. This phenomenon may be related to posttranscriptional gene silencing in transgenic plants and quelling in Neurospora and allows the efficient creation of specific mutant phenotypes thus providing a potentially powerful tool to study gene function in Paramecium. For the two multigene families that encode proteins that coassemble to build up complex subcellular structures the analysis presented herein provides the first experimental evidence that the members of these gene families are not functionally redundant. PMID:9529389

  20. Advances in study of reporter gene imaging for monitoring gene therapy

    International Nuclear Information System (INIS)

    Mu Chuanjie; Zhou Jiwen

    2003-01-01

    To evaluate the efficiency of gene therapy, it is requisite to monitor localization and expression of the therapeutic gene in vivo. Monitoring expression of reporter gene using radionuclide reporter gene technique is the best method. Adenoviral vectors expressing reporter gene are constructed using gene fusion, bicistronic, double promoter or bidirectional transcriptional recombination techniques, and transferred into target cells and tissues, then injected radiolabeled reporter probes which couple to the reporter genes. The reporter genes can be imaged invasively, repeatedly, quantitatively with γ-camera, PET and SPECT. Recently, several reporter gene and reporter probe systems have been used in studies of gene therapy. The part of them has been used for clinic trials

  1. Newer Gene Editing Technologies toward HIV Gene Therapy

    Directory of Open Access Journals (Sweden)

    Premlata Shankar

    2013-11-01

    Full Text Available Despite the great success of highly active antiretroviral therapy (HAART in ameliorating the course of HIV infection, alternative therapeutic approaches are being pursued because of practical problems associated with life-long therapy. The eradication of HIV in the so-called “Berlin patient” who received a bone marrow transplant from a CCR5-negative donor has rekindled interest in genome engineering strategies to achieve the same effect. Precise gene editing within the cells is now a realistic possibility with recent advances in understanding the DNA repair mechanisms, DNA interaction with transcription factors and bacterial defense mechanisms. Within the past few years, four novel technologies have emerged that can be engineered for recognition of specific DNA target sequences to enable site-specific gene editing: Homing Endonuclease, ZFN, TALEN, and CRISPR/Cas9 system. The most recent CRISPR/Cas9 system uses a short stretch of complementary RNA bound to Cas9 nuclease to recognize and cleave target DNA, as opposed to the previous technologies that use DNA binding motifs of either zinc finger proteins or transcription activator-like effector molecules fused to an endonuclease to mediate sequence-specific DNA cleavage. Unlike RNA interference, which requires the continued presence of effector moieties to maintain gene silencing, the newer technologies allow permanent disruption of the targeted gene after a single treatment. Here, we review the applications, limitations and future prospects of novel gene-editing strategies for use as HIV therapy.

  2. Sequencing and Gene Expression Analysis of Leishmania tropica LACK Gene.

    Science.gov (United States)

    Hammoudeh, Nour; Kweider, Mahmoud; Abbady, Abdul-Qader; Soukkarieh, Chadi

    2014-01-01

    Leishmania Homologue of receptors for Activated C Kinase (LACK) antigen is a 36-kDa protein, which provokes a very early immune response against Leishmania infection. There are several reports on the expression of LACK through different life-cycle stages of genus Leishmania, but only a few of them have focused on L.tropica. The present study provides details of the cloning, DNA sequencing and gene expression of LACK in this parasite species. First, several local isolates of Leishmania parasites were typed in our laboratory using PCR technique to verify of Leishmania parasite species. After that, LACK gene was amplified and cloned into a vector for sequencing. Finally, the expression of this molecule in logarithmic and stationary growth phase promastigotes, as well as in amastigotes, was evaluated by Reverse Transcription-PCR (RT-PCR) technique. The typing result confirmed that all our local isolates belong to L.tropica. LACK gene sequence was determined and high similarity was observed with the sequences of other Leishmania species. Furthermore, the expression of LACK gene in both promastigotes and amastigotes forms was confirmed. Overall, the data set the stage for future studies of the properties and immune role of LACK gene products.

  3. Reduced rates of gene loss, gene silencing, and gene mutation in Dnmt1-deficient embryonic stem cells

    NARCIS (Netherlands)

    Chan, M.F.; van Amerongen, R.; Nijjar, T.; Cuppen, E.; Jones, P.A.; Laird, P.W.

    2001-01-01

    Tumor suppressor gene inactivation is a crucial event in oncogenesis. Gene inactivation mechanisms include events resulting in loss of heterozygosity (LOH), gene mutation, and transcriptional silencing. The contribution of each of these different pathways varies among tumor suppressor genes and by

  4. Gene expression profiles in skeletal muscle after gene electrotransfer

    DEFF Research Database (Denmark)

    Hojman, Pernille; Zibert, John R; Gissel, Hanne

    2007-01-01

    ) followed by a long low voltage pulse (LV, 100 V/cm, 400 ms); a pulse combination optimised for efficient and safe gene transfer. Muscles were transfected with green fluorescent protein (GFP) and excised at 4 hours, 48 hours or 3 weeks after treatment. RESULTS: Differentially expressed genes were...... with the control muscles. Most interestingly, no changes in the expression of proteins involved in inflammatory responses or muscle regeneration was detected, indicating limited muscle damage and regeneration. Histological analysis revealed structural changes with loss of cell integrity and striation pattern......BACKGROUND: Gene transfer by electroporation (DNA electrotransfer) to muscle results in high level long term transgenic expression, showing great promise for treatment of e.g. protein deficiency syndromes. However little is known about the effects of DNA electrotransfer on muscle fibres. We have...

  5. Polymorphism of xenobiotic metabolizm genes

    International Nuclear Information System (INIS)

    Freidin, M. B.; Ten, I. A.; Sevostiyanova, N. v.; Kokorina, Y. I.; Slominskaya, F. M.; Tereshchenko, I. V.; Kolomiets, S. A.; Takhanov, R. M.

    2004-01-01

    The genes of xenobiotic metabolism enzymes genes, also called biotransformation genes or environmental genes seems to be an important factor of individual susceptibility of common diseases of different genesis including cancer. At the present time, a systematic accumulation of information on the role of these genes polymorphism in predisposition to different disorders is carried out worldwide. To investigate a significance of biotransformation genes for cancer disorders in West Siberia region, we studies a polymorphism of genes CYP2C19 (Small-RFLP, 1 and 2 alleles in exone 5), GSTTI, GSTMI (null and normal alleles in both genes) in 47 lung cancer (LC) patients, 269 women with breast cancer (BC), and 130 controls from Tomsk, Russia. All participants were Russians. The frequency of GSTM1 null genotype in LC patients was significantly higher than that in controls (0.608?0.071 vs. 0.162?0-032; p<1x10-7 by Fisher exact test), whereas the frequency of null genotype in BC women was significantly lower in comparison with control women (0.545?0.039 vs. 0.747?0.053: p=0.005 by Fisher exact test). The latter circumstance seems to be unexpected because a priori one suggest that just null genotype of GSTM1 is of a pathological nature. Frequencies of null genotypes of GSTN1 in LC patients and GSTT1 in BC women corresponded to those in controls. In LC patients frequencies of CYP2C19 alleles did not differ significantly from values control sample, whereas in BC women the frequency of CYP2C19 2 allele was higher than that in control (0.340?0.20 vs. 0.182?0.034, p=4,9x10-4 by Fisher exact test). This allele encodes a truncated non-functional protein being a major cause of poor metabolism of some drugs. Thus, the data obtained allow us to conclude that polymorphisms of GSTTi, GSTM1, and CYP2C19 genes are associated with cancer disorders in Russians from West Siberia Even so, their pathogenetic meaning is specific with respect the type of malignant pathology. (Author)

  6. Transcription Through Chromatin - Dynamic Organization of Genes

    Indian Academy of Sciences (India)

    Remodeling of chromatin confers it the ability for dynamic change. Remodeling is essential for transcriptional regulation, the first step of gene expression. Chromatin Structure and Gene Expression. Transcription is the first step of gene expression in which RNA synthesis occurs from the DNA (gene) template in a series of.

  7. Deregulated genes in sporadic vestibular schwannomas

    DEFF Research Database (Denmark)

    Cayé-Thomasen, Per; Helweg-Larsen, Rehannah Holga Andrea; Stangerup, Sven-Eric

    2010-01-01

    In search of genes associated with vestibular schwannoma tumorigenesis, this study examines the gene expression in human vestibular nerve versus vestibular schwannoma tissue samples using microarray technology.......In search of genes associated with vestibular schwannoma tumorigenesis, this study examines the gene expression in human vestibular nerve versus vestibular schwannoma tissue samples using microarray technology....

  8. Gene Synthesis with H G Khorana

    Indian Academy of Sciences (India)

    IAS Admin

    Why synthesize genes? Because of the naiveté of biologists and molecular biologists in 1965 when Gobind Khorana initiated synthesis of the yeast alanine tRNA gene, this was a valid question. At that time, we could deduce the sequence of only one gene – the yeast alanine tRNA gene as the corresponding tRNA.

  9. Transcription Through Chromatin - Dynamic Organization of Genes

    Indian Academy of Sciences (India)

    In this article, we discuss the dynamic organization of eukaryotic genes into chromatin. Remodeling of chromatin confers it the ability for dynamic change. Remodeling is essential for transcriptional regulation, the first step of gene expression. Chromatin Structure and Gene Expression. Transcription is the first step of gene ...

  10. Gene therapy for cystic fibrosis.

    Science.gov (United States)

    Mueller, Christian; Flotte, Terence R

    2008-12-01

    Cystic Fibrosis (CF) is an autosomal recessive disorder due to mutations in the CF transmembrane conductance regulator (CFTR) gene that lead to defective ion transport in the conducting pulmonary airways and exocrine glands. Through a process that is not fully understood, CFTR defects predispose affected patients to chronic endobronchial infections with organisms such as Pseudomonas aeruginosa and Staphylococcus aureus. Following the discovery of the CFTR gene in 1989, CF became one of the primary targets for gene therapy research. Early enthusiasm surrounded the new field of gene therapy during most of the 1990s and it led academics and clinicians on a big effort to apply gene therapy for cystic fibrosis. Clinical studies have been pursued using recombinant adenovirus, recombinant adeno-associated virus, cationic liposomes, and cationic polymer vectors. Although to this date no dramatic therapeutic benefits have been observed, a lot of information has been gained from the pre-clinical and clinical studies that were performed. This learning curve has led to the optimization of vector technology and an appreciation of immune and mechanical barriers that have to be overcome for successful delivery.

  11. Melatonin Receptor Genes in Vertebrates

    Directory of Open Access Journals (Sweden)

    Hua Dong Yin

    2013-05-01

    Full Text Available Melatonin receptors are members of the G protein-coupled receptor (GPCR family. Three genes for melatonin receptors have been cloned. The MT1 (or Mel1a or MTNR1A and MT2 (or Mel1b or MTNR1B receptor subtypes are present in humans and other mammals, while an additional melatonin receptor subtype, Mel1c (or MTNR1C, has been identified in fish, amphibians and birds. Another melatonin related orphan receptor, GPR50, which does not bind melatonin, is found exclusively in mammals. The hormone melatonin is secreted primarily by the pineal gland, with highest levels occurring during the dark period of a circadian cycle. This hormone acts systemically in numerous organs. In the brain, it is involved in the regulation of various neural and endocrine processes, and it readjusts the circadian pacemaker, the suprachiasmatic nucleus. This article reviews recent studies of gene organization, expression, evolution and mutations of melatonin receptor genes of vertebrates. Gene polymorphisms reveal that numerous mutations are associated with diseases and disorders. The phylogenetic analysis of receptor genes indicates that GPR50 is an outgroup to all other melatonin receptor sequences. GPR50 may have separated from a melatonin receptor ancestor before the split between MTNR1C and the MTNR1A/B ancestor.

  12. Gene Ontology Consortium: going forward.

    Science.gov (United States)

    2015-01-01

    The Gene Ontology (GO; http://www.geneontology.org) is a community-based bioinformatics resource that supplies information about gene product function using ontologies to represent biological knowledge. Here we describe improvements and expansions to several branches of the ontology, as well as updates that have allowed us to more efficiently disseminate the GO and capture feedback from the research community. The Gene Ontology Consortium (GOC) has expanded areas of the ontology such as cilia-related terms, cell-cycle terms and multicellular organism processes. We have also implemented new tools for generating ontology terms based on a set of logical rules making use of templates, and we have made efforts to increase our use of logical definitions. The GOC has a new and improved web site summarizing new developments and documentation, serving as a portal to GO data. Users can perform GO enrichment analysis, and search the GO for terms, annotations to gene products, and associated metadata across multiple species using the all-new AmiGO 2 browser. We encourage and welcome the input of the research community in all biological areas in our continued effort to improve the Gene Ontology. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

  13. Gene therapy for inherited immunodeficiency.

    Science.gov (United States)

    Touzot, Fabien; Hacein-Bey-Abina, Salima; Fischer, Alain; Cavazzana, Marina

    2014-06-01

    During the last decade, gene therapy has emerged as a convincing therapy for primary immunodeficiencies (PIDs). Ex vivo gene transfer into autologous hematopoietic stem cells (HSCs) via viral vectors permits sustained correction of T cell immunodeficiency in two forms of severe combined immunodeficiency: X-linked SCID (SCID-X1) (γ chain [γc] deficiency) and adenosine deaminase deficiency. However, this success has been balanced by the occurrence of genotoxicity generated by the integration of first-generation retroviral vectors. Recently, the development of safer self-inactivating vectors has led to the initiation of new studies with the hope of equivalent efficacy and a better safety profile. This review article focuses on the updated results of gene therapy trials for PIDs - from early studies to ongoing clinical trials. We detail the major advances made in gene transfer and repair technologies, and discuss the many ways to extend our present experience. With optimization in terms of safety and efficacy, gene therapy by lentiviral transduction could become a compelling alternative to allogeneic HSC transplantation, and thus may take center stage in the management of PIDs in coming years.

  14. Molecular Studies on Preproinsulin Gene

    Directory of Open Access Journals (Sweden)

    Sabir Sarah

    2016-01-01

    Full Text Available Insulin plays an important role in maintaining the blood glucose level of the body. The β-cells of pancreas produce insulin in the form of precursor that is preproinsulin. The gene of preproinsulin provides an interesting system for addressing question related to molecular evolution. Recombinant DNA technology has made it possible to isolate and sequence the chromosomal genes coding for unique protein products. Although preproinsulin of various organism has been isolated and cloned, but there is no report from buffalo (Bubalus bubalis that is our major livestock. The genomic DNA of buffalo was isolated using Laura-Lee-Boodram method. The part of preproinsulin gene (596bp and 520bp using BPPI-UPS and bpiful_F as forward and BC1-C as reverse primer was amplified. Cloning of amplified fragments of gene were performed in pCR 2.1 vector. Positive clones were screened on the basis of blue white selection. The band obtained on 596bp and 520bp after colony PCR confirmed the successful cloning of preproinsulin gene in pCR 2.1 vector.

  15. Gene methylation in gastric cancer.

    Science.gov (United States)

    Qu, Yiping; Dang, Siwen; Hou, Peng

    2013-09-23

    Gastric cancer is one of the most common malignancies and remains the second leading cause of cancer-related death worldwide. Over 70% of new cases and deaths occur in developing countries. In the early years of the molecular biology revolution, cancer research mainly focuses on genetic alterations, including gastric cancer. Epigenetic mechanisms are essential for normal development and maintenance of tissue-specific gene expression patterns in mammals. Disruption of epigenetic processes can lead to altered gene function and malignant cellular transformation. Recent advancements in the rapidly evolving field of cancer epigenetics have shown extensive reprogramming of every component of the epigenetic machinery in cancer, including DNA methylation, histone modifications, nucleosome positioning, noncoding RNAs, and microRNAs. Aberrant DNA methylation in the promoter regions of gene, which leads to inactivation of tumor suppressor and other cancer-related genes in cancer cells, is the most well-defined epigenetic hallmark in gastric cancer. The advantages of gene methylation as a target for detection and diagnosis of cancer in biopsy specimens and non-invasive body fluids such as serum and gastric washes have led to many studies of application in gastric cancer. This review focuses on the most common and important phenomenon of epigenetics, DNA methylation, in gastric cancer and illustrates the impact epigenetics has had on this field. Copyright © 2013 The Authors. Published by Elsevier B.V. All rights reserved.

  16. A hybrid approach of gene sets and single genes for the prediction of survival risks with gene expression data.

    Science.gov (United States)

    Seok, Junhee; Davis, Ronald W; Xiao, Wenzhong

    2015-01-01

    Accumulated biological knowledge is often encoded as gene sets, collections of genes associated with similar biological functions or pathways. The use of gene sets in the analyses of high-throughput gene expression data has been intensively studied and applied in clinical research. However, the main interest remains in finding modules of biological knowledge, or corresponding gene sets, significantly associated with disease conditions. Risk prediction from censored survival times using gene sets hasn't been well studied. In this work, we propose a hybrid method that uses both single gene and gene set information together to predict patient survival risks from gene expression profiles. In the proposed method, gene sets provide context-level information that is poorly reflected by single genes. Complementarily, single genes help to supplement incomplete information of gene sets due to our imperfect biomedical knowledge. Through the tests over multiple data sets of cancer and trauma injury, the proposed method showed robust and improved performance compared with the conventional approaches with only single genes or gene sets solely. Additionally, we examined the prediction result in the trauma injury data, and showed that the modules of biological knowledge used in the prediction by the proposed method were highly interpretable in biology. A wide range of survival prediction problems in clinical genomics is expected to benefit from the use of biological knowledge.

  17. Gene therapy and radiotherapy in malignant tumor

    International Nuclear Information System (INIS)

    Zhang Yaowen; Cao Yongzhen; Li Jin; Wang Qin

    2008-01-01

    Tumor treatment is one of the most important fields in medical research. Nowadays, a novel method which is combined gene therapy with radiotherapy plays an important role in the field of cancer research, and mainly includes immune gene therapy combined with radiotherapy, suicide gene therapy or tumor suppressor gene therapy combined with radiotherapy, antiangiogenesis gene therapy combined with radiotherapy and protective gene therapy combined with radiotherapy based on the technical features. This review summarized the current status of combined therapies of gene therapy and radiotherapy and possible mechanism. (authors)

  18. Correction of gene expression data

    DEFF Research Database (Denmark)

    Darbani Shirvanehdeh, Behrooz; Stewart, C. Neal, Jr.; Noeparvar, Shahin

    2014-01-01

    an analytical approach to examine the suitability of correction methods by considering the inter-treatment bias as well as the inter-replicate variance, which allows use of the best correction method with minimum residual bias. Analyses of RNA sequencing and microarray data showed that the efficiencies......This report investigates for the first time the potential inter-treatment bias source of cell number for gene expression studies. Cell-number bias can affect gene expression analysis when comparing samples with unequal total cellular RNA content or with different RNA extraction efficiencies....... For maximal reliability of analysis, therefore, comparisons should be performed at the cellular level. This could be accomplished using an appropriate correction method that can detect and remove the inter-treatment bias for cell-number. Based on inter-treatment variations of reference genes, we introduce...

  19. Gene Therapy in Cardiac Arrhythmias

    Directory of Open Access Journals (Sweden)

    Praveen S.V

    2006-04-01

    Full Text Available Gene therapy has progressed from a dream to a bedside reality in quite a few human diseases. From its first application in adenosine deaminase deficiency, through the years, its application has evolved to vascular angiogenesis and cardiac arrhythmias. Gene based biological pacemakers using viral vectors or mesenchymal cells tested in animal models hold much promise. Induction of pacemaker activity within the left bundle branch can provide stable heart rates. Genetic modification of the AV node mimicking beta blockade can be therapeutic in the management of atrial fibrillation. G protein overexpression to modify the AV node also is experimental. Modification and expression of potassium channel genes altering the delayed rectifier potassium currents may permit better management of congenital long QT syndromes. Arrhythmias in a failing heart are due to abnormal calcium cycling. Potential targets for genetic modulation include the sarcoplasmic reticulum calcium pump, calsequestrin and sodium calcium exchanger.Lastly the ethical concerns need to be addressed.

  20. Nonlinear dimensionality reduction of gene expression data

    OpenAIRE

    Nilsson, Jens

    2006-01-01

    Using microarray measurements techniques, it is possible to measure the activity of genes simultaneously across the whole genome. Since genes influence each others activity levels through complex regulatory networks, such gene expression measurements are state samples of a dynamical system. Gene expression data has proven useful for diagnosis and definition of disease subgroups, for inference of the functional role of a given gene or for the deciphering of complex disease mechanisms. However,...

  1. The KCNE genes in hypertrophic cardiomyopathy: a candidate gene study

    DEFF Research Database (Denmark)

    Hedley, Paula L; Haundrup, Ole; Andersen, Paal S

    2011-01-01

    as well as the T-tubules of the sarcolemma. It has been suggested that minK forms part of an "electro-mechanical feed-back" which links cardiomyocyte stretching to changes in ion channel function. We examined whether mutations in KCNE genes were associated with hypertrophic cardiomyopathy (HCM), a genetic...

  2. Gene expression analysis identifies global gene dosage sensitivity in cancer

    DEFF Research Database (Denmark)

    Fehrmann, Rudolf S. N.; Karjalainen, Juha M.; Krajewska, Malgorzata

    2015-01-01

    Many cancer-associated somatic copy number alterations (SCNAs) are known. Currently, one of the challenges is to identify the molecular downstream effects of these variants. Although several SCNAs are known to change gene expression levels, it is not clear whether each individual SCNA affects gen...

  3. Candidate Gene Identification of Flowering Time Genes in Cotton

    Directory of Open Access Journals (Sweden)

    Corrinne E. Grover

    2015-07-01

    Full Text Available Flowering time control is critically important to all sexually reproducing angiosperms in both natural ecological and agronomic settings. Accordingly, there is much interest in defining the genes involved in the complex flowering-time network and how these respond to natural and artificial selection, the latter often entailing transitions in day-length responses. Here we describe a candidate gene analysis in the cotton genus , which uses homologs from the well-described flowering network to bioinformatically and phylogenetically identify orthologs in the published genome sequence from Ulbr., one of the two model diploid progenitors of the commercially important allopolyploid cottons, L. and L. Presence and patterns of expression were evaluated from 13 aboveground tissues related to flowering for each of the candidate genes using allopolyploid as a model. Furthermore, we use a comparative context to determine copy number variability of each key gene family across 10 published angiosperm genomes. Data suggest a pattern of repeated loss of duplicates following ancient whole-genome doubling events in diverse lineages. The data presented here provide a foundation for understanding both the parallel evolution of day-length neutrality in domesticated cottons and the flowering-time network, in general, in this important crop plant.

  4. Effect Alpha Globlin Gene Deletion And Gamma Globin Gene -158 ...

    African Journals Online (AJOL)

    ... have been unable to find a molecular basis for the benign clinical course in all our patients. Other genetic or acquired factors must be hypothesized which ameliorate the clinical condition. Keywords: β- thalassemia, Xmn1 polymorphism, α-globin gene deletion. Egyptian Journal of Biochemistry and Molecular Biology Vol.

  5. FunGeneClusterS

    DEFF Research Database (Denmark)

    Vesth, Tammi Camilla; Brandl, Julian; Andersen, Mikael Rørdam

    2016-01-01

    and industrial biotechnology applications. We have previously published a method for accurate prediction of clusters from genome and transcriptome data, which could also suggest cross-chemistry, however, this method was limited both in the number of parameters which could be adjusted as well as in user......Secondary metabolites of fungi are receiving an increasing amount of interest due to their prolific bioactivities and the fact that fungal biosynthesis of secondary metabolites often occurs from co-regulated and co-located gene clusters. This makes the gene clusters attractive for synthetic biology...

  6. Mutated genes as research tool

    International Nuclear Information System (INIS)

    1981-01-01

    Green plants are the ultimate source of all resources required for man's life, his food, his clothes, and almost all his energy requirements. Primitive prehistoric man could live from the abundance of nature surrounding him. Man today, dominating nature in terms of numbers and exploiting its limited resources, cannot exist without employing his intelligence to direct natural evolution. Plant sciences, therefore, are not a matter of curiosity but an essential requirement. From such considerations, the IAEA and FAO jointly organized a symposium to assess the value of mutation research for various kinds of plant science, which directly or indirectly might contribute to sustaining and improving crop production. The benefit through developing better cultivars that plant breeders can derive from using the additional genetic resources resulting from mutation induction has been assessed before at other FAO/IAEA meetings (Rome 1964, Pullman 1969, Ban 1974, Ibadan 1978) and is also monitored in the Mutation Breeding Newsletter, published by IAEA twice a year. Several hundred plant cultivars which carry economically important characters because their genes have been altered by ionizing radiation or other mutagens, are grown by farmers and horticulturists in many parts of the world. But the benefit derived from such mutant varieties is without any doubt surpassed by the contribution which mutation research has made towards the advancement of genetics. For this reason, a major part of the papers and discussions at the symposium dealt with the role induced-mutation research played in providing insight into gene action and gene interaction, the organization of genes in plant chromosomes in view of homology and homoeology, the evolutionary role of gene duplication and polyploidy, the relevance of gene blocks, the possibilities for chromosome engineering, the functioning of cytroplasmic inheritance and the genetic dynamics of populations. In discussing the evolutionary role of

  7. Gene regulation by growth factors

    International Nuclear Information System (INIS)

    Metz, R.; Gorham, J.; Siegfried, Z.; Leonard, D.; Gizang-Ginsberg, E.; Thompson, M.A.; Lawe, D.; Kouzarides, T.; Vosatka, R.; MacGregor, D.; Jamal, S.; Greenberg, M.E.; Ziff, E.B.

    1988-01-01

    To coordinate the proliferation and differentiation of diverse cell types, cells of higher eukaryotes communicate through the release of growth factors. These peptides interact with specific transmembrane receptors of other cells and thereby generate intracellular messengers. The many changes in cellular physiology and activity that can be induced by growth factors imply that growth factor-induced signals can reach the nucleus and control gene activity. Moreover, current evidence also suggests that unregulated signaling along such pathways can induce aberrant proliferation and the formation of tumors. This paper reviews investigations of growth factor regulation of gene expression conducted by the authors' laboratory

  8. Gene therapy in cystic fibrosis.

    Science.gov (United States)

    Flotte, T R; Laube, B L

    2001-09-01

    Theoretically, cystic fibrosis transmembrane conductance regulator (CFTR) gene replacement during the neonatal period can decrease morbidity and mortality from cystic fibrosis (CF). In vivo gene transfers have been accomplished in CF patients. Choice of vector, mode of delivery to airways, translocation of genetic information, and sufficient expression level of the normalized CFTR gene are issues that currently are being addressed in the field. The advantages and limitations of viral vectors are a function of the parent virus. Viral vectors used in this setting include adenovirus (Ad) and adeno-associated virus (AAV). Initial studies with Ad vectors resulted in a vector that was efficient for gene transfer with dose-limiting inflammatory effects due to the large amount of viral protein delivered. The next generation of Ad vectors, with more viral coding sequence deletions, has a longer duration of activity and elicits a lesser degree of cell-mediated immunity in mice. A more recent generation of Ad vectors has no viral genes remaining. Despite these changes, the problem of humoral immunity remains with Ad vectors. A variety of strategies such as vector systems requiring single, or widely spaced, administrations, pharmacologic immunosuppression at administration, creation of a stealth vector, modification of immunogenic epitopes, or tolerance induction are being considered to circumvent humoral immunity. AAV vectors have been studied in animal and human models. They do not appear to induce inflammatory changes over a wide range of doses. The level of CFTR messenger RNA expression is difficult to ascertain with AAV vectors since the small size of the vector relative to the CFTR gene leaves no space for vector-specific sequences on which to base assays to distinguish endogenous from vector-expressed messenger RNA. In general, AAV vectors appear to be safe and have superior duration profiles. Cationic liposomes are lipid-DNA complexes. These vectors generally have been

  9. Multifactor dimensionality reduction for detecting gene-gene and gene-environment interactions in pharmacogenomics studies.

    Science.gov (United States)

    Ritchie, Marylyn D; Motsinger, Alison A

    2005-12-01

    In the quest for discovering disease susceptibility genes, the reality of gene-gene and gene-environment interactions creates difficult challenges for many current statistical approaches. In an attempt to overcome limitations with current disease gene detection methods, the multifactor dimensionality reduction (MDR) approach was previously developed. In brief, MDR is a method that reduces the dimensionality of multilocus information to identify polymorphisms associated with an increased risk of disease. This approach takes multilocus genotypes and develops a model for defining disease risk by pooling high-risk genotype combinations into one group and low-risk combinations into another. Cross-validation and permutation testing are used to identify optimal models. While this approach was initially developed for studies of complex disease, it is also directly applicable to pharmacogenomic studies where the outcome variable is drug treatment response/nonresponse or toxicity/no toxicity. MDR is a nonparametric and model-free approach that has been shown to have reasonable power to detect epistasis in both theoretical and empirical studies. This computational technology is described in detail in this review, and its application in pharmacogenomic studies is demonstrated.

  10. Gene and enhancer trap tagging of vascular-expressed genes in poplar trees

    Science.gov (United States)

    Andrew Groover; Joseph R. Fontana; Gayle Dupper; Caiping Ma; Robert Martienssen; Steven Strauss; Richard Meilan

    2004-01-01

    We report a gene discovery system for poplar trees based on gene and enhancer traps. Gene and enhancer trap vectors carrying the β-glucuronidase (GUS) reporter gene were inserted into the poplar genome via Agrobacterium tumefaciens transformation, where they reveal the expression pattern of genes at or near the insertion sites. Because GUS...

  11. Targeting the human lysozyme gene on bovine αs1- casein gene ...

    African Journals Online (AJOL)

    Targeting an exogenous gene into a favorable gene locus and for expression under endogenous regulators is an ideal method in mammary gland bioreactor research. For this purpose, a gene targeting vector was constructed to targeting the human lysozyme gene on bovine αs1-casein gene locus. In this case, the ...

  12. Targeting the human lysozyme gene on bovine αs1- casein gene ...

    African Journals Online (AJOL)

    ajl yemi

    2011-11-28

    Nov 28, 2011 ... Targeting an exogenous gene into a favorable gene locus and for expression under endogenous regulators is an ideal method in mammary gland bioreactor research. For this purpose, a gene targeting vector was constructed to targeting the human lysozyme gene on bovine αs1-casein gene locus. In this.

  13. Interactive visualization of gene regulatory networks with associated gene expression time series data

    NARCIS (Netherlands)

    Westenberg, Michel A.; Hijum, Sacha A.F.T. van; Lulko, Andrzej T.; Kuipers, Oscar P.; Roerdink, Jos B.T.M.; Linsen, L; Hagen, H; Hamann, B

    2008-01-01

    We present GENeVis, an application to visualize gene expression time series data in a gene regulatory network context. This is a network of regulator proteins that regulate the expression of their respective target genes. The networks are represented as graphs, in which the nodes represent genes,

  14. Homeobox gene expression in Brachiopoda

    DEFF Research Database (Denmark)

    Altenburger, Andreas; Martinez, Pedro; Wanninger, Andreas

    2011-01-01

    The molecular control that underlies brachiopod ontogeny is largely unknown. In order to contribute to this issue we analyzed the expression pattern of two homeobox containing genes, Not and Cdx, during development of the rhynchonelliform (i.e., articulate) brachiopod Terebratalia transversa. Not...

  15. Embryos, genes, and birth defects

    National Research Council Canada - National Science Library

    Ferretti, Patrizia

    2006-01-01

    ... Structural anomalies The genesis of chromosome abnormalities Embryo survival The cause of high levels of chromosome abnormality in human embryos Relative parental risks - age, translocations, inversions, gonadal and germinal mosaics 33 33 34 35 36 44 44 45 4 Identification and Analysis of Genes Involved in Congenital Malformation Syndromes Peter J. Scambler Ge...

  16. Meta-morpho-gene deposits

    International Nuclear Information System (INIS)

    Khasanov, A.Kh.

    1988-01-01

    Forming of meta-morpho-gene deposits make integral processes part of metamorphism and forming of proper rocks. The metamorphism of rocks and ores is essential modification of their mineral composition, structure and texture under the influence of rising of thermodynamical parameters

  17. Gene expression profile of pulpitis

    Science.gov (United States)

    Galicia, Johnah C.; Henson, Brett R.; Parker, Joel S.; Khan, Asma A.

    2016-01-01

    The cost, prevalence and pain associated with endodontic disease necessitate an understanding of the fundamental molecular aspects of its pathogenesis. This study was aimed to identify the genetic contributors to pulpal pain and inflammation. Inflamed pulps were collected from patients diagnosed with irreversible pulpitis (n=20). Normal pulps from teeth extracted for various reasons served as controls (n=20). Pain level was assessed using a visual analog scale (VAS). Genome-wide microarray analysis was performed using Affymetrix GeneTitan Multichannel Instrument. The difference in gene expression levels were determined by the Significance Analysis of Microarray program using a false discovery rate (q-value) of 5%. Genes involved in immune response, cytokine-cytokine receptor interaction and signaling, integrin cell surface interactions, and others were expressed at relatively higher levels in the in the pulpitis group. Moreover, several genes known to modulate pain and inflammation showed differential expression in asymptomatic and mild pain patients (≥30mm on VAS) compared to those with moderate to severe pain. This exploratory study provides a molecular basis for the clinical diagnosis of pulpitis. With an enhanced understanding of pulpal inflammation, future studies on treatment and management of pulpitis and on pain associated with it can have a biological reference to bridge treatment strategies with pulpal biology. PMID:27052691

  18. Genome position and gene amplification

    Czech Academy of Sciences Publication Activity Database

    Jirsová, Pavla; Snijders, A.M.; Kwek, S.; Roydasgupta, R.; Fridlyand, J.; Tokuyasu, T.; Pinkel, D.; Albertson, D. G.

    2007-01-01

    Roč. 8, č. 6 (2007), r120 ISSN 1474-760X Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : gene amplification * array comparative genomic hybridization * oncogene Subject RIV: BO - Biophysics Impact factor: 6.589, year: 2007

  19. Total Synthesis of a Gene

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 17; Issue 12. Total Synthesis of a Gene. H G Khorana. Classics Volume 17 Issue 12 December 2012 pp 1174-1197 ... Author Affiliations. H G Khorana1. Professor of Biology and Chemestry, Massachusetts Institute of Technology, Cambridge 02139.

  20. Genes, Environment, and Human Behavior.

    Science.gov (United States)

    Bloom, Mark V.; Cutter, Mary Ann; Davidson, Ronald; Dougherty, Michael J.; Drexler, Edward; Gelernter, Joel; McCullough, Laurence B.; McInerney, Joseph D.; Murray, Jeffrey C.; Vogler, George P.; Zola, John

    This curriculum module explores genes, environment, and human behavior. This book provides materials to teach about the nature and methods of studying human behavior, raise some of the ethical and public policy dilemmas emerging from the Human Genome Project, and provide professional development for teachers. An extensive Teacher Background…

  1. Gene expression profile of pulpitis.

    Science.gov (United States)

    Galicia, J C; Henson, B R; Parker, J S; Khan, A A

    2016-06-01

    The cost, prevalence and pain associated with endodontic disease necessitate an understanding of the fundamental molecular aspects of its pathogenesis. This study was aimed to identify the genetic contributors to pulpal pain and inflammation. Inflamed pulps were collected from patients diagnosed with irreversible pulpitis (n=20). Normal pulps from teeth extracted for various reasons served as controls (n=20). Pain level was assessed using a visual analog scale (VAS). Genome-wide microarray analysis was performed using Affymetrix GeneTitan Multichannel Instrument. The difference in gene expression levels were determined by the significance analysis of microarray program using a false discovery rate (q-value) of 5%. Genes involved in immune response, cytokine-cytokine receptor interaction and signaling, integrin cell surface interactions, and others were expressed at relatively higher levels in the pulpitis group. Moreover, several genes known to modulate pain and inflammation showed differential expression in asymptomatic and mild pain patients (⩾30 mm on VAS) compared with those with moderate to severe pain. This exploratory study provides a molecular basis for the clinical diagnosis of pulpitis. With an enhanced understanding of pulpal inflammation, future studies on treatment and management of pulpitis and on pain associated with it can have a biological reference to bridge treatment strategies with pulpal biology.

  2. Methylenetetrahydrofolate reductase (MTHFR) gene polymorphism ...

    African Journals Online (AJOL)

    Administrator

    2011-10-19

    Oct 19, 2011 ... Polymorphisms of the methylenetetrahydrofolate reductase (MTHFR) gene are associated with abortion, early embryo loss and recurrent spontaneous abortion in human. However, information on the association between MTHFR polymorphism and cow abortion is scarce. In the present study, the effects.

  3. Gene therapy of inherited immunodeficiencies.

    Science.gov (United States)

    Santilli, Giorgia; Thornhill, Susannah I; Kinnon, Christine; Thrasher, Adrian J

    2008-04-01

    Primary immunodeficiencies (PID) are a group of inherited diseases that affect the development or activity of the immune system. In severe cases allogeneic haematopoietic stem cell transplantation has proved to be a successful curative modality but it is limited by toxicity and reduced efficacy in mismatched donor settings. Gene therapy for PID has been developed as an alternative strategy and has entered the clinical arena. In this review we discuss the outcomes of recent gene therapy trials and some of the problems that remain to be tackled. Results from clinical trials for X-linked severe combined immunodeficiency (SCID-X1), adenosine deaminase deficient SCID (ADA-SCID), and X-linked chronic granulomatous disease (X-CGD) are discussed. In addition, other conditions are highlighted such as the Wiskott Aldrich Syndrome (WAS) for which gene therapy has shown considerable promise in preclinical studies, and are currently being translated into novel clinical approaches. Whilst these encouraging results demonstrate that gene therapy can be used successfully to treat monogenic PID, the occurrence of vector-related side effects has highlighted the need for accurate assessment of the associated risks and a requirement for improvements in vector design.

  4. Ethics of Gene Therapy Debated.

    Science.gov (United States)

    Borman, Stu

    1991-01-01

    Presented are the highlights of a press conference featuring biomedical ethicist LeRoy Walters of Georgetown University and attorney Andrew Kimbrell of the Foundation on Economic Trends. The opposing points of view of these two speakers serve to outline the pros and cons of the gene therapy issue. (CW)

  5. (TNNC1) gene in goat

    African Journals Online (AJOL)

    Yomi

    2012-02-23

    Feb 23, 2012 ... the protein conformation, which was viewed in the Swiss Pdb. Viewer (Schwede et al., 2003). Tissue expression analysis. Tissue expression pattern of TNNC1 was performed by fluorescence quantitative PCR (RT-PCR) using goat β-actin gene as an internal expression control. The primers of β-actin (Table ...

  6. Ancient genes of Saccharomyces cerevisiae.

    Science.gov (United States)

    Veiga-Crespo, P; Poza, M; Prieto-Alcedo, M; Villa, T G

    2004-07-01

    Amber is a plant resin mainly produced by coniferous trees that, after entrapping a variety of living beings, was subjected to a process of fossilization until it turned into yellowish, translucent stones. It is also one of the best sources of ancient DNA on which to perform studies on evolution. Here a method for the sterilization of amber that allows reliable ancient DNA extraction with no actual DNA contamination is described. Working with insects taken from amber, it was possible to amplify the ATP9, PGU1 and rRNA18S ancient genes of Saccharomyces cerevisiae corresponding to samples from the Miocene and Oligocene. After comparison of the current genes with their ancient (up to 35-40 million years) counterparts it was concluded that essential genes such as rRNA18S are highly conserved and that even normal 'house-keeping' genes, such as PGU1, are strikingly conserved along the millions of years that S. cerevisiae has evolved.

  7. (TNNC1) gene in goat

    African Journals Online (AJOL)

    Yomi

    2012-02-23

    Feb 23, 2012 ... nucleotide sequence was high and was similar in various animals ranging from 97.94 (cow) to 83.33%. (African clawed frog), and ... Key words: TroponinC1 gene, molecular cloning, structural analysis, expression, goat. INTRODUCTION ... influence on meat tenderness, in which the types of myofiber can be ...

  8. Candidate genes in panic disorder

    DEFF Research Database (Denmark)

    Howe, A. S.; Buttenschön, Henriette N; Bani-Fatemi, A.

    2016-01-01

    association studies and ancestry-specific effects. In the present study, we performed a succinct review of case-control association studies published prior to April 2015. Meta-analyses were performed for candidate gene variants examined in at least three studies using the Cochrane Mantel-Haenszel fixed...

  9. Genes and Syndromic Hearing Loss.

    Science.gov (United States)

    Keats, Bronya J. B.

    2002-01-01

    This article provides a description of the human genome and patterns of inheritance and discusses genes that are associated with some of the syndromes for which hearing loss is a common finding, including: Waardenburg, Stickler, Jervell and Lange-Neilsen, Usher, Alport, mitochondrial encephalomyopathy, and sensorineural hearing loss. (Contains…

  10. Using Genes to Guide Prescriptions

    Science.gov (United States)

    ... certain medicines. In general, each gene is the body's instructions for building a specific protein. These instructions are in a ... or different medications—to prevent the drug from building up to toxic levels before it's processed by the body. Rheumatoid Arthritis New insight into drugs that treat ...

  11. Homeobox genes and melatonin synthesis

    DEFF Research Database (Denmark)

    Rohde, Kristian; Møller, Morten; Rath, Martin Fredensborg

    2014-01-01

    Nocturnal synthesis of melatonin in the pineal gland is controlled by a circadian rhythm in arylalkylamine N-acetyltransferase (AANAT) enzyme activity. In the rodent, Aanat gene expression displays a marked circadian rhythm; release of norepinephrine in the gland at night causes a cAMP-based indu......Nocturnal synthesis of melatonin in the pineal gland is controlled by a circadian rhythm in arylalkylamine N-acetyltransferase (AANAT) enzyme activity. In the rodent, Aanat gene expression displays a marked circadian rhythm; release of norepinephrine in the gland at night causes a c......AMP-based induction of Aanat transcription. However, additional transcriptional control mechanisms exist. Homeobox genes, which are generally known to encode transcription factors controlling developmental processes, are also expressed in the mature rodent pineal gland. Among these, the cone-rod homeobox (CRX......) transcription factor is believed to control pineal-specific Aanat expression. Based on recent advances in our understanding of Crx in the rodent pineal gland, we here suggest that homeobox genes play a role in adult pineal physiology both by ensuring pineal-specific Aanat expression and by facilitating c...

  12. Gene Expression in Trypanosomatid Parasites

    Directory of Open Access Journals (Sweden)

    Santiago Martínez-Calvillo

    2010-01-01

    Full Text Available The parasites Leishmania spp., Trypanosoma brucei, and Trypanosoma cruzi are the trypanosomatid protozoa that cause the deadly human diseases leishmaniasis, African sleeping sickness, and Chagas disease, respectively. These organisms possess unique mechanisms for gene expression such as constitutive polycistronic transcription of protein-coding genes and trans-splicing. Little is known about either the DNA sequences or the proteins that are involved in the initiation and termination of transcription in trypanosomatids. In silico analyses of the genome databases of these parasites led to the identification of a small number of proteins involved in gene expression. However, functional studies have revealed that trypanosomatids have more general transcription factors than originally estimated. Many posttranslational histone modifications, histone variants, and chromatin modifying enzymes have been identified in trypanosomatids, and recent genome-wide studies showed that epigenetic regulation might play a very important role in gene expression in this group of parasites. Here, we review and comment on the most recent findings related to transcription initiation and termination in trypanosomatid protozoa.

  13. Methylenetetrahydrofolate reductase (MTHFR) gene polymorphism ...

    African Journals Online (AJOL)

    Polymorphisms of the methylenetetrahydrofolate reductase (MTHFR) gene are associated with abortion, early embryo loss and recurrent spontaneous abortion in human. However, information on the association between MTHFR polymorphism and cow abortion is scarce. In the present study, the effects of MTHFR ...

  14. Gene editing. Time to reflection.

    OpenAIRE

    Santaló Pedro, Josep

    2017-01-01

    The development of new gene editing technologies and their special characteristics have led to a passionate debate on the suitability and reliability of their use both in plant and animal species and in the human species itself. A brief analysis of the arguments used in this debate as well as a summary of some of the most relevant statements in this regard is made.

  15. Gene therapy for lung cancer.

    Science.gov (United States)

    Toloza, Eric M; Morse, Michael A; Lyerly, H Kim

    2006-09-01

    Lung cancer patients suffer a 15% overall survival despite advances in chemotherapy, radiation therapy, and surgery. This unacceptably low survival rate is due to the usual finding of advanced disease at diagnosis. However, multimodality strategies using conventional therapies only minimally improve survival rates even in early stages of lung cancer. Attempts to improve survival in advanced disease using various combinations of platinum-based chemotherapy have demonstrated that no regimen is superior, suggesting a therapeutic plateau and the need for novel, more specific, and less toxic therapeutic strategies. Over the past three decades, the genetic etiology of cancer has been gradually delineated, albeit not yet completely. Understanding the molecular events that occur during the multistep process of bronchogenic carcinogenesis may make these tasks more surmountable. During these same three decades, techniques have been developed which allow transfer of functional genes into mammalian cells. For example, blockade of activated tumor-promoting oncogenes or replacement of inactivated tumor-suppressing or apoptosis-promoting genes can be achieved by gene therapy. This article will discuss the therapeutic implications of these molecular changes associated with bronchogenic carcinomas and will then review the status of gene therapies for treatment of lung cancer. (c) 2006 Wiley-Liss, Inc.

  16. Genome-wide Analysis of Gene Regulation

    DEFF Research Database (Denmark)

    Chen, Yun

    cells are capable of regulating their gene expression, so that each cell can only express a particular set of genes yielding limited numbers of proteins with specialized functions. Therefore a rigid control of differential gene expression is necessary for cellular diversity. On the other hand, aberrant...... gene regulation will disrupt the cell’s fundamental processes, which in turn can cause disease. Hence, understanding gene regulation is essential for deciphering the code of life. Along with the development of high throughput sequencing (HTS) technology and the subsequent large-scale data analysis......, genome-wide assays have increased our understanding of gene regulation significantly. This thesis describes the integration and analysis of HTS data across different important aspects of gene regulation. Gene expression can be regulated at different stages when the genetic information is passed from gene...

  17. Gene conversion in the rice genome

    DEFF Research Database (Denmark)

    Xu, Shuqing; Clark, Terry; Zheng, Hongkun

    2008-01-01

    BACKGROUND: Gene conversion causes a non-reciprocal transfer of genetic information between similar sequences. Gene conversion can both homogenize genes and recruit point mutations thereby shaping the evolution of multigene families. In the rice genome, the large number of duplicated genes...... increases opportunities for gene conversion. RESULTS: To characterize gene conversion in rice, we have defined 626 multigene families in which 377 gene conversions were detected using the GENECONV program. Over 60% of the conversions we detected were between chromosomes. We found that the inter......-chromosomal conversions distributed between chromosome 1 and 5, 2 and 6, and 3 and 5 are more frequent than genome average (Z-test, P conversion on the same chromosome decreased with the physical distance between gene conversion partners. Ka/Ks analysis indicates that gene conversion...

  18. QTLminer: identifying genes regulating quantitative traits

    Directory of Open Access Journals (Sweden)

    Schughart Klaus

    2010-10-01

    Full Text Available Abstract Background Quantitative trait locus (QTL mapping identifies genomic regions that likely contain genes regulating a quantitative trait. However, QTL regions may encompass tens to hundreds of genes. To find the most promising candidate genes that regulate the trait, the biologist typically collects information from multiple resources about the genes in the QTL interval. This process is very laborious and time consuming. Results QTLminer is a bioinformatics tool that automatically performs QTL region analysis. It is available in GeneNetwork and it integrates information such as gene annotation, gene expression and sequence polymorphisms for all the genes within a given genomic interval. Conclusions QTLminer substantially speeds up discovery of the most promising candidate genes within a QTL region.

  19. Gene expression profiles in skeletal muscle after gene electrotransfer

    Directory of Open Access Journals (Sweden)

    Eriksen Jens

    2007-06-01

    Full Text Available Abstract Background Gene transfer by electroporation (DNA electrotransfer to muscle results in high level long term transgenic expression, showing great promise for treatment of e.g. protein deficiency syndromes. However little is known about the effects of DNA electrotransfer on muscle fibres. We have therefore investigated transcriptional changes through gene expression profile analyses, morphological changes by histological analysis, and physiological changes by force generation measurements. DNA electrotransfer was obtained using a combination of a short high voltage pulse (HV, 1000 V/cm, 100 μs followed by a long low voltage pulse (LV, 100 V/cm, 400 ms; a pulse combination optimised for efficient and safe gene transfer. Muscles were transfected with green fluorescent protein (GFP and excised at 4 hours, 48 hours or 3 weeks after treatment. Results Differentially expressed genes were investigated by microarray analysis, and descriptive statistics were performed to evaluate the effects of 1 electroporation, 2 DNA injection, and 3 time after treatment. The biological significance of the results was assessed by gene annotation and supervised cluster analysis. Generally, electroporation caused down-regulation of structural proteins e.g. sarcospan and catalytic enzymes. Injection of DNA induced down-regulation of intracellular transport proteins e.g. sentrin. The effects on muscle fibres were transient as the expression profiles 3 weeks after treatment were closely related with the control muscles. Most interestingly, no changes in the expression of proteins involved in inflammatory responses or muscle regeneration was detected, indicating limited muscle damage and regeneration. Histological analysis revealed structural changes with loss of cell integrity and striation pattern in some fibres after DNA+HV+LV treatment, while HV+LV pulses alone showed preservation of cell integrity. No difference in the force generation capacity was observed in

  20. MGC: a metagenomic gene caller.

    Science.gov (United States)

    El Allali, Achraf; Rose, John R

    2013-01-01

    Computational gene finding algorithms have proven their robustness in identifying genes in complete genomes. However, metagenomic sequencing has presented new challenges due to the incomplete and fragmented nature of the data. During the last few years, attempts have been made to extract complete and incomplete open reading frames (ORFs) directly from short reads and identify the coding ORFs, bypassing other challenging tasks such as the assembly of the metagenome. In this paper we introduce a metagenomics gene caller (MGC) which is an improvement over the state-of-the-art prediction algorithm Orphelia. Orphelia uses a two-stage machine learning approach and computes a model that classifies extracted ORFs from fragmented sequences. We hypothesise and demonstrate evidence that sequences need separate models based on their local GC-content in order to avoid the noise introduced to a single model computed with sequences from the entire GC spectrum. We have also added two amino-acid features based on the benefit of amino-acid usage shown in our previous research. Our algorithm is able to predict genes and translation initiation sites (TIS) more accurately than Orphelia which uses a single model. Learning separate models for several pre-defined GC-content regions as opposed to a single model approach improves the performance of the neural network as demonstrated by the experimental results presented in this paper. The inclusion of amino-acid usage features also helps improve the overall accuracy of our algorithm. MGC's improvement sets the ground for further investigation into the use of GC-content to separate data for training models in machine learning based gene finders.

  1. Transferring alien genes to wheat

    International Nuclear Information System (INIS)

    Knott, D.R.

    1987-01-01

    In broad terms an alien gene can be considered to be any gene transferred to wheat from a related species. As described above by Maan (section 7D) the genus Triticum contains a broad range of species, some of which cross readily with the cultivated tetraploid (T. Turgidum L.) or hexaploid (T. aestivum L.) wheats, and others only with great difficulty. In addition, wheat will also cross with species in a number of other genera including Agropyron, Elymus, Elytrigia (=Agropyron), Haynaldia, Hordeum, and Secale (Riley and Kimber, 1966; Knobloch, 1968; Feldman and Sears, 1981). In discussing the Triticum and Aegilops spp., the classification by Kimber and Sears, section SA-I, above, will be followed. For the Agropyron and related species the classification described by Dewey (1983) will be used. To avoid confusion, in referring to the literature the designations used by the authors will be given, followed by the new designation. The wild relatives of wheat are adapted to a broad range of environments and carry a large reservoir of useful genes (Zohary et al., 1969; Kerber and Dyck, 1973; Brezhnev, 1977; Feldman and Sears, 1981; Limin and Fowler, 1981; Sharma et aI., 1981; McGuire and Dvorak, 1981). Initially they were considered to be primarily sources of disease resistance, but more recently they have been recognized as potential sources of genes for high protein, cold tolerance, salt tolerance, drought tolerance, lodging resistance, early maturity, and even yield. Extensive screening of the wild relatives of wheat needs to be done before their useful genes can be fully utilized

  2. Vascular Gene Expression: A Hypothesis

    Directory of Open Access Journals (Sweden)

    Angélica Concepción eMartínez-Navarro

    2013-07-01

    Full Text Available The phloem is the conduit through which photoassimilates are distributed from autotrophic to heterotrophic tissues and is involved in the distribution of signaling molecules that coordinate plant growth and responses to the environment. Phloem function depends on the coordinate expression of a large array of genes. We have previously identified conserved motifs in upstream regions of the Arabidopsis genes, encoding the homologs of pumpkin phloem sap mRNAs, displaying expression in vascular tissues. This tissue-specific expression in Arabidopsis is predicted by the overrepresentation of GA/CT-rich motifs in gene promoters. In this work we have searched for common motifs in upstream regions of the homologous genes from plants considered to possess a primitive vascular tissue (a lycophyte, as well as from others that lack a true vascular tissue (a bryophyte, and finally from chlorophytes. Both lycophyte and bryophyte display motifs similar to those found in Arabidopsis with a significantly low E-value, while the chlorophytes showed either a different conserved motif or no conserved motif at all. These results suggest that these same genes are expressed coordinately in non- vascular plants; this coordinate expression may have been one of the prerequisites for the development of conducting tissues in plants. We have also analyzed the phylogeny of conserved proteins that may be involved in phloem function and development. The presence of CmPP16, APL, FT and YDA in chlorophytes suggests the recruitment of ancient regulatory networks for the development of the vascular tissue during evolution while OPS is a novel protein specific to vascular plants.

  3. Gene losses during human origins.

    Directory of Open Access Journals (Sweden)

    Xiaoxia Wang

    2006-03-01

    Full Text Available Pseudogenization is a widespread phenomenon in genome evolution, and it has been proposed to serve as an engine of evolutionary change, especially during human origins (the "less-is-more" hypothesis. However, there has been no comprehensive analysis of human-specific pseudogenes. Furthermore, it is unclear whether pseudogenization itself can be selectively favored and thus play an active role in human evolution. Here we conduct a comparative genomic analysis and a literature survey to identify 80 nonprocessed pseudogenes that were inactivated in the human lineage after its separation from the chimpanzee lineage. Many functions are involved among these genes, with chemoreception and immune response being outstandingly overrepresented, suggesting potential species-specific features in these aspects of human physiology. To explore the possibility of adaptive pseudogenization, we focus on CASPASE12, a cysteinyl aspartate proteinase participating in inflammatory and innate immune response to endotoxins. We provide population genetic evidence that the nearly complete fixation of a null allele at CASPASE12 has been driven by positive selection, probably because the null allele confers protection from severe sepsis. We estimate that the selective advantage of the null allele is about 0.9% and the pseudogenization started shortly before the out-of-Africa migration of modern humans. Interestingly, two other genes related to sepsis were also pseudogenized in humans, possibly by selection. These adaptive gene losses might have occurred because of changes in our environment or genetic background that altered the threat from or response to sepsis. The identification and analysis of human-specific pseudogenes open the door for understanding the roles of gene losses in human origins, and the demonstration that gene loss itself can be adaptive supports and extends the "less-is-more" hypothesis.

  4. Empirical study of supervised gene screening

    Directory of Open Access Journals (Sweden)

    Ma Shuangge

    2006-12-01

    Full Text Available Abstract Background Microarray studies provide a way of linking variations of phenotypes with their genetic causations. Constructing predictive models using high dimensional microarray measurements usually consists of three steps: (1 unsupervised gene screening; (2 supervised gene screening; and (3 statistical model building. Supervised gene screening based on marginal gene ranking is commonly used to reduce the number of genes in the model building. Various simple statistics, such as t-statistic or signal to noise ratio, have been used to rank genes in the supervised screening. Despite of its extensive usage, statistical study of supervised gene screening remains scarce. Our study is partly motivated by the differences in gene discovery results caused by using different supervised gene screening methods. Results We investigate concordance and reproducibility of supervised gene screening based on eight commonly used marginal statistics. Concordance is assessed by the relative fractions of overlaps between top ranked genes screened using different marginal statistics. We propose a Bootstrap Reproducibility Index, which measures reproducibility of individual genes under the supervised screening. Empirical studies are based on four public microarray data. We consider the cases where the top 20%, 40% and 60% genes are screened. Conclusion From a gene discovery point of view, the effect of supervised gene screening based on different marginal statistics cannot be ignored. Empirical studies show that (1 genes passed different supervised screenings may be considerably different; (2 concordance may vary, depending on the underlying data structure and percentage of selected genes; (3 evaluated with the Bootstrap Reproducibility Index, genes passed supervised screenings are only moderately reproducible; and (4 concordance cannot be improved by supervised screening based on reproducibility.

  5. Gene transfer therapy in vascular diseases.

    Science.gov (United States)

    McKay, M J; Gaballa, M A

    2001-01-01

    Somatic gene therapy of vascular diseases is a promising new field in modern medicine. Recent advancements in gene transfer technology have greatly evolved our understanding of the pathophysiologic role of candidate disease genes. With this knowledge, the expression of selective gene products provides the means to test the therapeutic use of gene therapy in a multitude of medical conditions. In addition, with the completion of genome sequencing programs, gene transfer can be used also to study the biologic function of novel genes in vivo. Novel genes are delivered to targeted tissue via several different vehicles. These vectors include adenoviruses, retroviruses, plasmids, plasmid/liposomes, and oligonucleotides. However, each one of these vectors has inherent limitations. Further investigations into developing delivery systems that not only allow for efficient, targeted gene transfer, but also are stable and nonimmunogenic, will optimize the clinical application of gene therapy in vascular diseases. This review further discusses the available mode of gene delivery and examines six major areas in vascular gene therapy, namely prevention of restenosis, thrombosis, hypertension, atherosclerosis, peripheral vascular disease in congestive heart failure, and ischemia. Although we highlight some of the recent advances in the use of gene therapy in treating vascular disease discovered primarily during the past two years, many excellent studies published during that period are not included in this review due to space limitations. The following is a selective review of practical uses of gene transfer therapy in vascular diseases. This review primarily covers work performed in the last 2 years. For earlier work, the reader may refer to several excellent review articles. For instance, Belalcazer et al. (6) reviewed general aspects of somatic gene therapy and the different vehicles used for the delivery of therapeutic genes. Gene therapy in restenosis and stimulation of

  6. Gene transfer: anything goes in plant mitochondria

    Directory of Open Access Journals (Sweden)

    Richards Thomas A

    2010-12-01

    Full Text Available Abstract Parasitic plants and their hosts have proven remarkably adept at exchanging fragments of mitochondrial DNA. Two recent studies provide important mechanistic insights into the pattern, process and consequences of horizontal gene transfer, demonstrating that genes can be transferred in large chunks and that gene conversion between foreign and native genes leads to intragenic mosaicism. A model involving duplicative horizontal gene transfer and differential gene conversion is proposed as a hitherto unrecognized source of genetic diversity. See research article: http://www.biomedcentral.com/1741-7007/8/150

  7. Gene transfer: anything goes in plant mitochondria

    Science.gov (United States)

    2010-01-01

    Parasitic plants and their hosts have proven remarkably adept at exchanging fragments of mitochondrial DNA. Two recent studies provide important mechanistic insights into the pattern, process and consequences of horizontal gene transfer, demonstrating that genes can be transferred in large chunks and that gene conversion between foreign and native genes leads to intragenic mosaicism. A model involving duplicative horizontal gene transfer and differential gene conversion is proposed as a hitherto unrecognized source of genetic diversity. See research article: http://www.biomedcentral.com/1741-7007/8/150 PMID:21176244

  8. Modulation of gene expression made easy

    DEFF Research Database (Denmark)

    Solem, Christian; Jensen, Peter Ruhdal

    2002-01-01

    A new approach for modulating gene expression, based on randomization of promoter (spacer) sequences, was developed. The method was applied to chromosomal genes in Lactococcus lactis and shown to generate libraries of clones with broad ranges of expression levels of target genes. In one example...... that the method can be applied to modulating the expression of native genes on the chromosome. We constructed a series of strains in which the expression of the las operon, containing the genes pfk, pyk, and ldh, was modulated by integrating a truncated copy of the pfk gene. Importantly, the modulation affected...

  9. Investigation progress of PET reporter gene imaging

    International Nuclear Information System (INIS)

    Chen Yumei; Huang Gang

    2006-01-01

    Molecular imaging for gene therapy and gene expression has been more and more attractive, while the use of gene therapy has been widely investigated and intense research have allowed it to the clinical setting in the last two-decade years. In vivo imaging with positron emission tomography (PET) by combination of appropriate PET reporter gene and PET reporter probe could provide qualitative and quantitative information for gene therapy. PET imaging could also obtain some valuable parameters not available by other techniques. This technology is useful to understand the process and development of gene therapy and how to apply it into clinical practice in the future. (authors)

  10. Cerebrovascular gene expression in spontaneously hypertensive rats

    DEFF Research Database (Denmark)

    Grell, Anne-Sofie; Frederiksen, Simona Denise; Edvinsson, Lars

    2017-01-01

    in the middle cerebral arteries from hypertensive compared to normotensive rats. The gene expression of 72 genes was decreased and the gene expression of 97 genes was increased. The following genes with a fold difference ≥1.40 were verified by quantitative PCR; Postn, Olr1, Fas, Vldlr, Mmp2, Timp1, Serpine1......, Mmp11, Cd34, Ptgs1 and Ptgs2. The gene expression of Postn, Olr1, Fas, Vldlr, Mmp2, Timp1 and Serpine1 and the protein expression of LOX1 (also known as OLR1) were significantly increased in the middle cerebral arteries from spontaneously hypertensive rats compared to Wistar-Kyoto rats. In conclusion...

  11. The relationship among gene expression, the evolution of gene dosage, and the rate of protein evolution.

    Directory of Open Access Journals (Sweden)

    Jean-François Gout

    2010-05-01

    Full Text Available The understanding of selective constraints affecting genes is a major issue in biology. It is well established that gene expression level is a major determinant of the rate of protein evolution, but the reasons for this relationship remain highly debated. Here we demonstrate that gene expression is also a major determinant of the evolution of gene dosage: the rate of gene losses after whole genome duplications in the Paramecium lineage is negatively correlated to the level of gene expression, and this relationship is not a byproduct of other factors known to affect the fate of gene duplicates. This indicates that changes in gene dosage are generally more deleterious for highly expressed genes. This rule also holds for other taxa: in yeast, we find a clear relationship between gene expression level and the fitness impact of reduction in gene dosage. To explain these observations, we propose a model based on the fact that the optimal expression level of a gene corresponds to a trade-off between the benefit and cost of its expression. This COSTEX model predicts that selective pressure against mutations changing gene expression level or affecting the encoded protein should on average be stronger in highly expressed genes and hence that both the frequency of gene loss and the rate of protein evolution should correlate negatively with gene expression. Thus, the COSTEX model provides a simple and common explanation for the general relationship observed between the level of gene expression and the different facets of gene evolution.

  12. GenePRIMP: A GENE PRediction IMprovement Pipeline for Prokaryotic genomes

    Energy Technology Data Exchange (ETDEWEB)

    Pati, Amrita; Ivanova, Natalia N.; Mikhailova, Natalia; Ovchinnikova, Galina; Hooper, Sean D.; Lykidis, Athanasios; Kyrpides, Nikos C.

    2010-04-01

    We present 'gene prediction improvement pipeline' (GenePRIMP; http://geneprimp.jgi-psf.org/), a computational process that performs evidence-based evaluation of gene models in prokaryotic genomes and reports anomalies including inconsistent start sites, missed genes and split genes. We found that manual curation of gene models using the anomaly reports generated by GenePRIMP improved their quality, and demonstrate the applicability of GenePRIMP in improving finishing quality and comparing different genome-sequencing and annotation technologies.

  13. Multifactor dimensionality reduction software for detecting gene-gene and gene-environment interactions.

    Science.gov (United States)

    Hahn, Lance W; Ritchie, Marylyn D; Moore, Jason H

    2003-02-12

    Polymorphisms in human genes are being described in remarkable numbers. Determining which polymorphisms and which environmental factors are associated with common, complex diseases has become a daunting task. This is partly because the effect of any single genetic variation will likely be dependent on other genetic variations (gene-gene interaction or epistasis) and environmental factors (gene-environment interaction). Detecting and characterizing interactions among multiple factors is both a statistical and a computational challenge. To address this problem, we have developed a multifactor dimensionality reduction (MDR) method for collapsing high-dimensional genetic data into a single dimension thus permitting interactions to be detected in relatively small sample sizes. In this paper, we describe the MDR approach and an MDR software package. We developed a program that integrates MDR with a cross-validation strategy for estimating the classification and prediction error of multifactor models. The software can be used to analyze interactions among 2-15 genetic and/or environmental factors. The dataset may contain up to 500 total variables and a maximum of 4000 study subjects. Information on obtaining the executable code, example data, example analysis, and documentation is available upon request. All supplementary information can be found at http://phg.mc.vanderbilt.edu/Software/MDR.

  14. Gene family matters: expanding the HGNC resource

    Directory of Open Access Journals (Sweden)

    Daugherty Louise C

    2012-07-01

    Full Text Available Abstract The HUGO Gene Nomenclature Committee (HGNC assigns approved gene symbols to human loci. There are currently over 33,000 approved gene symbols, the majority of which represent protein-coding genes, but we also name other locus types such as non-coding RNAs, pseudogenes and phenotypic loci. Where relevant, the HGNC organise these genes into gene families and groups. The HGNC website http://www.genenames.org/ is an online repository of HGNC-approved gene nomenclature and associated resources for human genes, and includes links to genomic, proteomic and phenotypic information. In addition to this, we also have dedicated gene family web pages and are currently expanding and generating more of these pages using data curated by the HGNC and from information derived from external resources that focus on particular gene families. Here, we review our current online resources with a particular focus on our gene family data, using it to highlight our new Gene Symbol Report and gene family data downloads.

  15. Thesaurus-based disambiguation of gene symbols

    Directory of Open Access Journals (Sweden)

    Wain Hester M

    2005-06-01

    Full Text Available Abstract Background Massive text mining of the biological literature holds great promise of relating disparate information and discovering new knowledge. However, disambiguation of gene symbols is a major bottleneck. Results We developed a simple thesaurus-based disambiguation algorithm that can operate with very little training data. The thesaurus comprises the information from five human genetic databases and MeSH. The extent of the homonym problem for human gene symbols is shown to be substantial (33% of the genes in our combined thesaurus had one or more ambiguous symbols, not only because one symbol can refer to multiple genes, but also because a gene symbol can have many non-gene meanings. A test set of 52,529 Medline abstracts, containing 690 ambiguous human gene symbols taken from OMIM, was automatically generated. Overall accuracy of the disambiguation algorithm was up to 92.7% on the test set. Conclusion The ambiguity of human gene symbols is substantial, not only because one symbol may denote multiple genes but particularly because many symbols have other, non-gene meanings. The proposed disambiguation approach resolves most ambiguities in our test set with high accuracy, including the important gene/not a gene decisions. The algorithm is fast and scalable, enabling gene-symbol disambiguation in massive text mining applications.

  16. Effect of gene order in DNA constructs on gene expression upon integration into plant genome.

    Science.gov (United States)

    Aydın Akbudak, M; Srivastava, Vibha

    2017-06-01

    Several plant biotechnology applications are based on the expression of multiple genes located on a single transformation vector. The principles of stable expression of foreign genes in plant cells include integration of full-length gene fragments consisting of promoter and transcription terminator sequences, and avoiding converging orientation of the gene transcriptional direction. Therefore, investigators usually generate constructs in which genes are assembled in the same orientation. However, no specific information is available on the effect of the order in which genes should be assembled in the construct to support optimum expression of each gene upon integration in the genome. While many factors, including genomic position and the integration structure, could affect gene expression, the investigators judiciously design DNA constructs to avoid glitches. However, the gene order in a multigene assembly remains an open question. This study addressed the effect of gene order in the DNA construct on gene expression in rice using a simple design of two genes placed in two possible orders with respect to the genomic context. Transgenic rice lines containing green fluorescent protein (GFP) and β-glucuronidase (GUS) genes in two distinct orders were developed by Cre-lox-mediated site-specific integration. Gene expression analysis of transgenic lines showed that both genes were expressed at similar levels in either orientation, and different transgenic lines expressed each gene within 1-2× range. Thus, no significant effect of the gene order on gene expression was found in the transformed rice lines containing precise site-specific integrations and stable gene expression in plant cells could be obtained with altered gene orders. Therefore, gene orientation and integration structures are more important factors governing gene expression than gene orders in the genomic context.

  17. Research progress in machine learning methods for gene-gene interaction detection.

    Science.gov (United States)

    Peng, Zhe-Ye; Tang, Zi-Jun; Xie, Min-Zhu

    2018-03-20

    Complex diseases are results of gene-gene and gene-environment interactions. However, the detection of high-dimensional gene-gene interactions is computationally challenging. In the last two decades, machine-learning approaches have been developed to detect gene-gene interactions with some successes. In this review, we summarize the progress in research on machine learning methods, as applied to gene-gene interaction detection. It systematically examines the principles and limitations of the current machine learning methods used in genome wide association studies (GWAS) to detect gene-gene interactions, such as neural networks (NN), random forest (RF), support vector machines (SVM) and multifactor dimensionality reduction (MDR), and provides some insights on the future research directions in the field.

  18. Mutation analysis of the preproghrelin gene

    DEFF Research Database (Denmark)

    Larsen, Lesli H; Gjesing, Anette P; Sørensen, Thorkild I A

    2005-01-01

    To investigate the preproghrelin gene for variants and their association with obesity and type 2 diabetes.......To investigate the preproghrelin gene for variants and their association with obesity and type 2 diabetes....

  19. In The Genes? Searching for Methuselah

    Science.gov (United States)

    ... Current Issue Past Issues Special Section In The Genes? Searching for Methuselah Past Issues / Winter 2007 Table ... 18 million effort to learn more about the genes, lifestyle or other factors that contribute to long, ...

  20. Cattle Candidate Genes for Milk Production Traits

    OpenAIRE

    KADLEC, Tomáš

    2012-01-01

    The aim of this thesis is to make an overview of important candidate genes affecting milk yield and milk quality parameters, with an emphasis on genes associated with the quantity and quality of milk proteins and milk fat.

  1. Overexpression of CFH gene in pterygiumv patients

    African Journals Online (AJOL)

    generation RNA sequencing. Significant gene lists were obtained using DAVID, GSEA, and KEGG for enriched pathway analyses of differentially expressed genes. Real- time polymerase chain reaction was performed to validate differential expressions ...

  2. Biodegradable nanoparticles for gene therapy technology

    International Nuclear Information System (INIS)

    Hosseinkhani, Hossein; He, Wen-Jie; Chiang, Chiao-Hsi; Hong, Po-Da; Yu, Dah-Shyong; Domb, Abraham J.; Ou, Keng-Liang

    2013-01-01

    Rapid propagations in materials technology together with biology have initiated great hopes in the possibility of treating many diseases by gene therapy technology. Viral and non-viral gene carriers are currently applied for gene delivery. Non-viral technology is safe and effective for the delivery of genetic materials to cells and tissues. Non-viral systems are based on plasmid expression containing a gene encoding a therapeutic protein and synthetic biodegradable nanoparticles as a safe carrier of gene. Biodegradable nanoparticles have shown great interest in drug and gene delivery systems as they are easy to be synthesized and have no side effect in cells and tissues. This review provides a critical view of applications of biodegradable nanoparticles on gene therapy technology to enhance the localization of in vitro and in vivo and improve the function of administered genes

  3. NIH Researchers Identify OCD Risk Gene

    Science.gov (United States)

    ... Issues Research News From NIH NIH Researchers Identify OCD Risk Gene Past Issues / Summer 2006 Table of ... gene variant that doubles an individual's risk for obsessive-compulsive disorder (OCD). The new functional variant, or allele, is ...

  4. Gene Therapy Approaches to Immunodeficiency.

    Science.gov (United States)

    Ghosh, Sujal; Gaspar, H Bobby

    2017-10-01

    Transfer of gene-corrected autologous hematopoietic stem cells in patients with primary immunodeficiencies has emerged as a new therapeutic approach. Patients with various conditions lacking a suitable donor have been treated with retroviral vectors and a gene-addition strategy. Initial promising results were shadowed by the occurrence of malignancies in some of these patients. Current trials, developed in the last decade, use safer viral vectors to overcome the risk of genotoxicity and have led to improved clinical outcomes. This review reflects the progresses made in specific disorders, including adenosine deaminase deficiency, X-linked severe combined immunodeficiency, chronic granulomatous disease, and Wiskott-Aldrich syndrome. Copyright © 2017 Elsevier Inc. All rights reserved.

  5. Function analysis of unknown genes

    DEFF Research Database (Denmark)

    Rogowska-Wrzesinska, A.

    2002-01-01

      This thesis entitled "Function analysis of unknown genes" presents the use of proteome analysis for the characterisation of yeast (Saccharomyces cerevisiae) genes and their products (proteins especially those of unknown function). This study illustrates that proteome analysis can be used...... be obtained using proteome analysis. Chapter 1 and 2 provide the basic theoretical aspects of proteome analysis, its principles, the main techniques involved and their use in the studies of the molecular biology of yeast cells. Chapter 3 presents the methods and tools involved in proteome analysis and used...... presents a comparison of the proteomes of three yeast wild type strains CEN.PK2, FY1679 and W303 that are widely used in function analysis projects and proves that FY1679 and W303 strains are more similar to each other than to the CEN.PK2 strain. This study identifies 62 proteins that are differentially...

  6. Phenotypic deconstruction of gene circuitry.

    Science.gov (United States)

    Lomnitz, Jason G; Savageau, Michael A

    2013-06-01

    It remains a challenge to obtain a global perspective on the behavioral repertoire of complex nonlinear gene circuits. In this paper, we describe a method for deconstructing complex systems into nonlinear sub-systems, based on mathematically defined phenotypes, which are then represented within a system design space that allows the repertoire of qualitatively distinct phenotypes of the complex system to be identified, enumerated, and analyzed. This method efficiently characterizes large regions of system design space and quickly generates alternative hypotheses for experimental testing. We describe the motivation and strategy in general terms, illustrate its use with a detailed example involving a two-gene circuit with a rich repertoire of dynamic behavior, and discuss experimental means of navigating the system design space.

  7. Phenotypic deconstruction of gene circuitry

    Science.gov (United States)

    Lomnitz, Jason G.; Savageau, Michael A.

    2013-06-01

    It remains a challenge to obtain a global perspective on the behavioral repertoire of complex nonlinear gene circuits. In this paper, we describe a method for deconstructing complex systems into nonlinear sub-systems, based on mathematically defined phenotypes, which are then represented within a system design space that allows the repertoire of qualitatively distinct phenotypes of the complex system to be identified, enumerated, and analyzed. This method efficiently characterizes large regions of system design space and quickly generates alternative hypotheses for experimental testing. We describe the motivation and strategy in general terms, illustrate its use with a detailed example involving a two-gene circuit with a rich repertoire of dynamic behavior, and discuss experimental means of navigating the system design space.

  8. Religious coalition opposes gene patents.

    Science.gov (United States)

    James, J S

    1995-05-19

    The biotechnology industry is concerned about a coalition of mainstream religious leaders, working with Jeremy Rifkin of the Foundation of Economic Trends, who oppose the patenting of human and animal life forms, body parts, and genes. The coalition called a press conference on May 18 to ask the government to prohibit the current patenting practices for genetic engineering. The biotechnology industry argues that patents indicate that a company's research tool has significant value, and encourages capitalists to invest their dollars in the development of new treatments for diseases. They also argue that the 29 biotech drugs that are on the market have been developed as a result of patents on genes. Although most business leaders are united in opposing restrictions, many scientists are divided, citing both religious and scientific reasons.

  9. Rotavirus gene structure and function.

    OpenAIRE

    Estes, M K; Cohen, J

    1989-01-01

    Knowledge of the structure and function of the genes and proteins of the rotaviruses has expanded rapidly. Information obtained in the last 5 years has revealed unexpected and unique molecular properties of rotavirus proteins of general interest to virologists, biochemists, and cell biologists. Rotaviruses share some features of replication with reoviruses, yet antigenic and molecular properties of the outer capsid proteins, VP4 (a protein whose cleavage is required for infectivity, possibly ...

  10. Gene Therapy for Childhood Neurofibromatosis

    Science.gov (United States)

    2014-05-01

    Neurofibromatosis PRINCIPAL INVESTIGATOR: Segal, David J. CONTRACTING ORGANIZATION: University of California, Davis Davis, California...May 2014 4. TITLE AND SUBTITLE Gene Therapy for Childhood Neurofibromatosis 5a. CONTRACT NUMBER 5b. GRANT NUMBER W81XWH-13-1-0101 5c...project was to develop an innovative therapy for neurofibromatosis . Neurofibromatosis type 1 (NF1) is one of the most common genetic disorders (1

  11. Organization of an echinoderm Hox gene cluster

    OpenAIRE

    Martinez, Pedro; Rast, Jonathan P.; Arenas-Mena, César; Davidson, Eric H.

    1999-01-01

    The Strongylocentrotus purpuratus genome contains a single ten-gene Hox complex >0.5 megabase in length. This complex was isolated on overlapping bacterial artificial chromosome and P1 artificial chromosome genomic recombinants by using probes for individual genes and by genomic walking. Echinoderm Hox genes of Paralog Groups (PG) 1 and 2 are reported. The cluster includes genes representing all paralog groups of vertebrate Hox clusters, except that there is a sing...

  12. Gene therapy: theoretical and bioethical concepts.

    Science.gov (United States)

    Smith, Kevin R

    2003-01-01

    Gene therapy holds great promise. Somatic gene therapy has the potential to treat a wide range of disorders, including inherited conditions, cancers, and infectious diseases. Early progress has already been made in the treatment of a range of disorders. Ethical issues surrounding somatic gene therapy are primarily those concerned with safety. Germline gene therapy is theoretically possible but raises serious ethical concerns concerning future generations.

  13. Gene coexpression network analysis as a source of functional annotation for rice genes.

    Directory of Open Access Journals (Sweden)

    Kevin L Childs

    Full Text Available With the existence of large publicly available plant gene expression data sets, many groups have undertaken data analyses to construct gene coexpression networks and functionally annotate genes. Often, a large compendium of unrelated or condition-independent expression data is used to construct gene networks. Condition-dependent expression experiments consisting of well-defined conditions/treatments have also been used to create coexpression networks to help examine particular biological processes. Gene networks derived from either condition-dependent or condition-independent data can be difficult to interpret if a large number of genes and connections are present. However, algorithms exist to identify modules of highly connected and biologically relevant genes within coexpression networks. In this study, we have used publicly available rice (Oryza sativa gene expression data to create gene coexpression networks using both condition-dependent and condition-independent data and have identified gene modules within these networks using the Weighted Gene Coexpression Network Analysis method. We compared the number of genes assigned to modules and the biological interpretability of gene coexpression modules to assess the utility of condition-dependent and condition-independent gene coexpression networks. For the purpose of providing functional annotation to rice genes, we found that gene modules identified by coexpression analysis of condition-dependent gene expression experiments to be more useful than gene modules identified by analysis of a condition-independent data set. We have incorporated our results into the MSU Rice Genome Annotation Project database as additional expression-based annotation for 13,537 genes, 2,980 of which lack a functional annotation description. These results provide two new types of functional annotation for our database. Genes in modules are now associated with groups of genes that constitute a collective functional

  14. Posttranscriptional gene silencing in nuclei

    Science.gov (United States)

    Hoffer, Paul; Ivashuta, Sergey; Pontes, Olga; Vitins, Alexa; Pikaard, Craig; Mroczka, Andrew; Wagner, Nicholas; Voelker, Toni

    2011-01-01

    In plants, small interfering RNAs (siRNAs) with sequence homology to transcribed regions of genes can guide the sequence-specific degradation of corresponding mRNAs, leading to posttranscriptional gene silencing (PTGS). The current consensus is that siRNA-mediated PTGS occurs primarily in the cytoplasm where target mRNAs are localized and translated into proteins. However, expression of an inverted-repeat double-stranded RNA corresponding to the soybean FAD2-1A desaturase intron is sufficient to silence FAD2-1, implicating nuclear precursor mRNA (pre-mRNA) rather than cytosolic mRNA as the target of PTGS. Silencing FAD2-1 using intronic or 3′-UTR sequences does not affect transcription rates of the target genes but results in the strong reduction of target transcript levels in the nucleus. Moreover, siRNAs corresponding to pre-mRNA–specific sequences accumulate in the nucleus. In Arabidopsis, we find that two enzymes involved in PTGS, Dicer-like 4 and RNA-dependent RNA polymerase 6, are localized in the nucleus. Collectively, these results demonstrate that siRNA-directed RNA degradation can take place in the nucleus, suggesting the need for a more complex view of the subcellular compartmentation of PTGS in plants. PMID:21173264

  15. Gene adaptation to extreme environments

    International Nuclear Information System (INIS)

    Marlaire, P.; Rodriguez, V.; Kerner, N.

    2005-01-01

    Full text: This work is oriented to the study of gene adaptation to extreme conditions, such as the hydrothermal system located in Copahue, Neuquen, Argentina. The organisms living there develop under two pressure selection conditions: the high temperature of thermal water and the strong impact of ultraviolet (UV) radiation. Several microorganisms found in this region were isolated and different colonies resistant to UV radiation were selected, a Geobacillus thermoleovorans strain identified through 16S RNA sequence, being the most remarkable. A gene library was prepared out of this strain with UV sensitive bacteria BH200 (uvrA::Tn10). A number of clones were isolated by means of UV selection, the most outstanding being a gene carrier able to codify for the guanosine monophosphate synthetase enzyme (GMPs). The suitability of said enzyme was proved by means of additional assays performed on ght 1 bacteria (guaA26::Tn 10) which lacked the enzyme. A transcript of 1100 pb was detected through Northern Blot. The result was consistent with that obtained for the mapping of the starting transcription site. The cloned GMPs produces an increase in growth speed and a greater biomass in BH200 bacteria. (author)

  16. Repetitive sequence environment distinguishes housekeeping genes.

    Science.gov (United States)

    Eller, C Daniel; Regelson, Moira; Merriman, Barry; Nelson, Stan; Horvath, Steve; Marahrens, York

    2007-04-01

    Housekeeping genes are expressed across a wide variety of tissues. Since repetitive sequences have been reported to influence the expression of individual genes, we employed a novel approach to determine whether housekeeping genes can be distinguished from tissue-specific genes by their repetitive sequence context. We show that Alu elements are more highly concentrated around housekeeping genes while various longer (>400-bp) repetitive sequences ("repeats"), including Long Interspersed Nuclear Element-1 (LINE-1) elements, are excluded from these regions. We further show that isochore membership does not distinguish housekeeping genes from tissue-specific genes and that repetitive sequence environment distinguishes housekeeping genes from tissue-specific genes in every isochore. The distinct repetitive sequence environment, in combination with other previously published sequence properties of housekeeping genes, was used to develop a method of predicting housekeeping genes on the basis of DNA sequence alone. Using expression across tissue types as a measure of success, we demonstrate that repetitive sequence environment is by far the most important sequence feature identified to date for distinguishing housekeeping genes.

  17. (GDF9) gene in Assam Hill goat

    African Journals Online (AJOL)

    Assam hill goat (Capra hircus) is a prolific local goat in India. Growth differentiation factor (GDF9) gene was studied as a candidate gene for the prolificacy of goats. The objective of the present study was to detect the incidence of mutation in the exonic region of GDF9 gene of Assam hill goat. Total number of 90 blood ...

  18. Gene therapy in India: A focus

    Indian Academy of Sciences (India)

    /fulltext/jbsc/039/03/0537-0541. Keywords. Genetic diseases; gene therapy; viral vectors. Abstract. Gene therapy refers to the treatment of genetic diseases using normal copies of the defective genes. It has the potential to cure any genetic ...

  19. Uses of antimicrobial genes from microbial genome

    Science.gov (United States)

    Sorek, Rotem; Rubin, Edward M.

    2013-08-20

    We describe a method for mining microbial genomes to discover antimicrobial genes and proteins having broad spectrum of activity. Also described are antimicrobial genes and their expression products from various microbial genomes that were found using this method. The products of such genes can be used as antimicrobial agents or as tools for molecular biology.

  20. Structure of the murine Thy-1 gene

    NARCIS (Netherlands)

    V. Giguere; K-I. Isobe; F.G. Grosveld (Frank)

    1985-01-01

    textabstractWe have cloned the murine Thy-1.1 (AKR) and Thy-1.2 (Balb/c) genes. The complete exon/intron structure and the nucleotide sequence of the Thy-1.2 gene was determined. The gene contains four exons and three intervening sequences. The complete transcriptional unit gives rise to a tissue

  1. Development of detection method for novel fusion gene using GeneChip exon array.

    Science.gov (United States)

    Wada, Yusaku; Matsuura, Masaaki; Sugawara, Minoru; Ushijima, Masaru; Miyata, Satoshi; Nagasaki, Koichi; Noda, Tetsuo; Miki, Yoshio

    2014-02-18

    Fusion genes have been recognized to play key roles in oncogenesis. Though, many techniques have been developed for genome-wide analysis of fusion genes, a more efficient method is desired. We introduced a new method of detecting the novel fusion gene by using GeneChip Exon Array that enables exon expression analysis on a whole-genome scale and TAIL-PCR. To screen genes with abnormal exon expression profiles, we developed computational program, and confirmed that the program was able to search the fusion partner gene using Exon Array data of T-cell acute lymphocytic leukemia (T-ALL) cell lines. It was reported that the T-ALL cell lines, ALL-SIL, BE13 and LOUCY, harbored the fusion gene NUP214-ABL1, NUP214-ABL1 and SET-NUP214, respectively. The program extracted the candidate genes with abnormal exon expression profiles: 1 gene in ALL-SIL, 1 gene in BE13, and 2 genes in LOUCY. The known fusion partner gene NUP214 was included in the genes in ALL-SIL and LOUCY. Thus, we applied the proposed program to the detection of fusion partner genes in other tumors. To discover novel fusion genes, we examined 24 breast cancer cell lines and 20 pancreatic cancer cell lines by using the program. As a result, 20 and 23 candidate genes were obtained for the breast and pancreatic cancer cell lines respectively, and seven genes were selected as the final candidate gene based on information of the EST data base, comparison with normal cell samples and visual inspection of Exon expression profile. Finding of fusion partners for the final candidate genes was tried by TAIL-PCR, and three novel fusion genes were identified. The usefulness of our detection method was confirmed. Using this method for more samples, it is thought that fusion genes can be identified.

  2. Gene-gene and gene-environment interactions in prostate, breast and colorectal cancer

    DEFF Research Database (Denmark)

    Kopp, Tine Iskov

    The incidence of cancer in the western world has increased steeply during the last 50 years. For three of the most prevalent cancer types in Denmark, prostate, breast and colorectal cancer (PC, BC and CRC, respectively), only a small fraction (1-15%) of the incidences are caused by highly penetrant......, such as alcohol consumption, smoking, obesity, inflammation and high meat intake; whereas other factors protect against cancer, such as high intake of dietary fibre, fruits and vegetables, and physical activity. Investigating the interactions between genetic variations and environmental factors, such as dietary...... was used to examine gene-gene and geneenvironment interactions in relation to risk of cancer (Paper I-V). A human intervention trial (Paper V) was conducted in order to directly examine the effect on concurrent use of nonsteroidal anti-inflammatory drugs (NSAIDs) and alcohol consumption on circulating...

  3. Using RNA-Seq data to select refence genes for normalizing gene expression in apple roots

    Science.gov (United States)

    Gene expression in apple roots in response to various stress conditions is a less-explored research subject. Reliable reference genes for normalizing quantitative gene expression data have not been carefully investigated. In this study, the suitability of a set of 15 apple genes were evaluated for t...

  4. Reranking candidate gene models with cross-species comparison for improved gene prediction

    Directory of Open Access Journals (Sweden)

    Pereira Fernando CN

    2008-10-01

    Full Text Available Abstract Background Most gene finders score candidate gene models with state-based methods, typically HMMs, by combining local properties (coding potential, splice donor and acceptor patterns, etc. Competing models with similar state-based scores may be distinguishable with additional information. In particular, functional and comparative genomics datasets may help to select among competing models of comparable probability by exploiting features likely to be associated with the correct gene models, such as conserved exon/intron structure or protein sequence features. Results We have investigated the utility of a simple post-processing step for selecting among a set of alternative gene models, using global scoring rules to rerank competing models for more accurate prediction. For each gene locus, we first generate the K best candidate gene models using the gene finder Evigan, and then rerank these models using comparisons with putative orthologous genes from closely-related species. Candidate gene models with lower scores in the original gene finder may be selected if they exhibit strong similarity to probable orthologs in coding sequence, splice site location, or signal peptide occurrence. Experiments on Drosophila melanogaster demonstrate that reranking based on cross-species comparison outperforms the best gene models identified by Evigan alone, and also outperforms the comparative gene finders GeneWise and Augustus+. Conclusion Reranking gene models with cross-species comparison improves gene prediction accuracy. This straightforward method can be readily adapted to incorporate additional lines of evidence, as it requires only a ranked source of candidate gene models.

  5. Comparative Genomic Analysis of Soybean Flowering Genes

    Science.gov (United States)

    Jung, Chol-Hee; Wong, Chui E.; Singh, Mohan B.; Bhalla, Prem L.

    2012-01-01

    Flowering is an important agronomic trait that determines crop yield. Soybean is a major oilseed legume crop used for human and animal feed. Legumes have unique vegetative and floral complexities. Our understanding of the molecular basis of flower initiation and development in legumes is limited. Here, we address this by using a computational approach to examine flowering regulatory genes in the soybean genome in comparison to the most studied model plant, Arabidopsis. For this comparison, a genome-wide analysis of orthologue groups was performed, followed by an in silico gene expression analysis of the identified soybean flowering genes. Phylogenetic analyses of the gene families highlighted the evolutionary relationships among these candidates. Our study identified key flowering genes in soybean and indicates that the vernalisation and the ambient-temperature pathways seem to be the most variant in soybean. A comparison of the orthologue groups containing flowering genes indicated that, on average, each Arabidopsis flowering gene has 2-3 orthologous copies in soybean. Our analysis highlighted that the CDF3, VRN1, SVP, AP3 and PIF3 genes are paralogue-rich genes in soybean. Furthermore, the genome mapping of the soybean flowering genes showed that these genes are scattered randomly across the genome. A paralogue comparison indicated that the soybean genes comprising the largest orthologue group are clustered in a 1.4 Mb region on chromosome 16 of soybean. Furthermore, a comparison with the undomesticated soybean (Glycine soja) revealed that there are hundreds of SNPs that are associated with putative soybean flowering genes and that there are structural variants that may affect the genes of the light-signalling and ambient-temperature pathways in soybean. Our study provides a framework for the soybean flowering pathway and insights into the relationship and evolution of flowering genes between a short-day soybean and the long-day plant, Arabidopsis. PMID:22679494

  6. Integrating Ontological Knowledge and Textual Evidence in Estimating Gene and Gene Product Similarity

    Energy Technology Data Exchange (ETDEWEB)

    Sanfilippo, Antonio P.; Posse, Christian; Gopalan, Banu; Tratz, Stephen C.; Gregory, Michelle L.

    2006-06-08

    With the rising influence of the Gene On-tology, new approaches have emerged where the similarity between genes or gene products is obtained by comparing Gene Ontology code annotations associ-ated with them. So far, these approaches have solely relied on the knowledge en-coded in the Gene Ontology and the gene annotations associated with the Gene On-tology database. The goal of this paper is to demonstrate that improvements to these approaches can be obtained by integrating textual evidence extracted from relevant biomedical literature.

  7. Synthetic promoter libraries- tuning of gene expression

    DEFF Research Database (Denmark)

    Hammer, Karin; Mijakovic, Ivan; Jensen, Peter Ruhdal

    2006-01-01

    The study of gene function often requires changing the expression of a gene and evaluating the consequences. In principle, the expression of any given gene can be modulated in a quasi-continuum of discrete expression levels but the traditional approaches are usually limited to two extremes: gene...... be met by using promoter libraries. This approach generally consists of inserting a library of promoters in front of the gene to be studied, whereby the individual promoters might deviate either in their spacer sequences or bear slight deviations from the consensus sequence of a vegetative promoter. Here......, we describe the two different methods for obtaining promoter libraries and compare their applicability....

  8. Positron emission tomography imaging of gene expression

    International Nuclear Information System (INIS)

    Tang Ganghua

    2001-01-01

    The merging of molecular biology and nuclear medicine is developed into molecular nuclear medicine. Positron emission tomography (PET) of gene expression in molecular nuclear medicine has become an attractive area. Positron emission tomography imaging gene expression includes the antisense PET imaging and the reporter gene PET imaging. It is likely that the antisense PET imaging will lag behind the reporter gene PET imaging because of the numerous issues that have not yet to be resolved with this approach. The reporter gene PET imaging has wide application into animal experimental research and human applications of this approach will likely be reported soon

  9. A review for detecting gene-gene interactions using machine learning methods in genetic epidemiology.

    Science.gov (United States)

    Koo, Ching Lee; Liew, Mei Jing; Mohamad, Mohd Saberi; Salleh, Abdul Hakim Mohamed

    2013-01-01

    Recently, the greatest statistical computational challenge in genetic epidemiology is to identify and characterize the genes that interact with other genes and environment factors that bring the effect on complex multifactorial disease. These gene-gene interactions are also denoted as epitasis in which this phenomenon cannot be solved by traditional statistical method due to the high dimensionality of the data and the occurrence of multiple polymorphism. Hence, there are several machine learning methods to solve such problems by identifying such susceptibility gene which are neural networks (NNs), support vector machine (SVM), and random forests (RFs) in such common and multifactorial disease. This paper gives an overview on machine learning methods, describing the methodology of each machine learning methods and its application in detecting gene-gene and gene-environment interactions. Lastly, this paper discussed each machine learning method and presents the strengths and weaknesses of each machine learning method in detecting gene-gene interactions in complex human disease.

  10. Mining Association Rules among Gene Functions in Clusters of Similar Gene Expression Maps.

    Science.gov (United States)

    An, Li; Obradovic, Zoran; Smith, Desmond; Bodenreider, Olivier; Megalooikonomou, Vasileios

    2009-11-01

    Association rules mining methods have been recently applied to gene expression data analysis to reveal relationships between genes and different conditions and features. However, not much effort has focused on detecting the relation between gene expression maps and related gene functions. Here we describe such an approach to mine association rules among gene functions in clusters of similar gene expression maps on mouse brain. The experimental results show that the detected association rules make sense biologically. By inspecting the obtained clusters and the genes having the gene functions of frequent itemsets, interesting clues were discovered that provide valuable insight to biological scientists. Moreover, discovered association rules can be potentially used to predict gene functions based on similarity of gene expression maps.

  11. [The application of multifactor dimensionality reduction for detecting gene-gene interactions].

    Science.gov (United States)

    Tang, Xun; Li, Na; Hu, Yong-hua

    2006-05-01

    To introduce the application of Multifactor Dimensionality Reduction (MDR) method for detecting gene-gene interactions in genetic case-control studies. A brief overview on basic steps involved in the implementation, theoretical details, available software as well as the use and features of the MDR method were discussed based on a practical research case. Advantages of MDR were compared to the conventional statistical approaches, showing that MDR method was a novel, nonparametric, genetic model-free approach that was developed specifically for detecting gene-gene interactions. Theoretical and empirical studies suggested that MDR was having reasonable power for detecting gene-gene interactions. Applications of MDR method had found the evidence of gene-gene interactions in several diseases such as sporadic breast cancer, atrial fibrillation and essential hypertension. MDR method could be used for detecting gene-gene interactions in genetic case-control studies as having great advantages versus the conventional statistical approaches.

  12. Validation of reference genes for quantifying changes in gene expression in virus-infected tobacco.

    Science.gov (United States)

    Baek, Eseul; Yoon, Ju-Yeon; Palukaitis, Peter

    2017-10-01

    To facilitate quantification of gene expression changes in virus-infected tobacco plants, eight housekeeping genes were evaluated for their stability of expression during infection by one of three systemically-infecting viruses (cucumber mosaic virus, potato virus X, potato virus Y) or a hypersensitive-response-inducing virus (tobacco mosaic virus; TMV) limited to the inoculated leaf. Five reference-gene validation programs were used to establish the order of the most stable genes for the systemically-infecting viruses as ribosomal protein L25 > β-Tubulin > Actin, and the least stable genes Ubiquitin-conjugating enzyme (UCE) genes were EF1α > Cysteine protease > Actin, and the least stable genes were GAPDH genes, three defense responsive genes were examined to compare their relative changes in gene expression caused by each virus. Copyright © 2017 Elsevier Inc. All rights reserved.

  13. Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family.

    Science.gov (United States)

    Teunissen, A W; Steensma, H Y

    1995-09-15

    The quality of brewing strains is, in large part, determined by their flocculation properties. By classical genetics, several dominant, semidominant and recessive flocculation genes have been recognized. Recent results of experiments to localize the flocculation genes FLO5 and FLO8, combined with the in silicio analysis of the available sequence data of the yeast genome, have revealed that the flocculation genes belong to a family which comprises at least four genes and three pseudogenes. All members of this gene family are located near the end of chromosomes, just like the SUC, MEL and MAL genes, which are also important for good quality baking or brewing strains. Transcription of the flocculation genes is repressed by several regulatory genes. In addition, a number of genes have been found which cause cell aggregation upon disruption or overexpression in an as yet unknown manner. In total, 33 genes have been reported that are involved in flocculation or cell aggregation.

  14. Progress in Gene Therapy for Prostate Cancer

    International Nuclear Information System (INIS)

    Ahmed, Kamran A.; Davis, Brian J.; Wilson, Torrence M.; Wiseman, Gregory A.; Federspiel, Mark J.; Morris, John C.

    2012-01-01

    Gene therapy has held promise to correct various disease processes. Prostate cancer represents the second leading cause of cancer death in American men. A number of clinical trials involving gene therapy for the treatment of prostate cancer have been reported. The ability to efficiently transduce tumors with effective levels of therapeutic genes has been identified as a fundamental barrier to effective cancer gene therapy. The approach utilizing gene therapy in prostate cancer patients at our institution attempts to address this deficiency. The sodium-iodide symporter (NIS) is responsible for the ability of the thyroid gland to transport and concentrate iodide. The characteristics of the NIS gene suggest that it could represent an ideal therapeutic gene for cancer therapy. Published results from Mayo Clinic researchers have indicated several important successes with the use of the NIS gene and prostate gene therapy. Studies have demonstrated that transfer of the human NIS gene into prostate cancer using adenovirus vectors in vitro and in vivo results in efficient uptake of radioactive iodine and significant tumor growth delay with prolongation of survival. Preclinical successes have culminated in the opening of a phase I trial for patients with advanced prostate disease which is currently accruing patients. Further study will reveal the clinical promise of NIS gene therapy in the treatment of prostate as well as other malignancies.

  15. Bioinformatics study of the mangrove actin genes

    Science.gov (United States)

    Basyuni, M.; Wasilah, M.; Sumardi

    2017-01-01

    This study describes the bioinformatics methods to analyze eight actin genes from mangrove plants on DDBJ/EMBL/GenBank as well as predicted the structure, composition, subcellular localization, similarity, and phylogenetic. The physical and chemical properties of eight mangroves showed variation among the genes. The percentage of the secondary structure of eight mangrove actin genes followed the order of a helix > random coil > extended chain structure for BgActl, KcActl, RsActl, and A. corniculatum Act. In contrast to this observation, the remaining actin genes were random coil > extended chain structure > a helix. This study, therefore, shown the prediction of secondary structure was performed for necessary structural information. The values of chloroplast or signal peptide or mitochondrial target were too small, indicated that no chloroplast or mitochondrial transit peptide or signal peptide of secretion pathway in mangrove actin genes. These results suggested the importance of understanding the diversity and functional of properties of the different amino acids in mangrove actin genes. To clarify the relationship among the mangrove actin gene, a phylogenetic tree was constructed. Three groups of mangrove actin genes were formed, the first group contains B. gymnorrhiza BgAct and R. stylosa RsActl. The second cluster which consists of 5 actin genes the largest group, and the last branch consist of one gene, B. sexagula Act. The present study, therefore, supported the previous results that plant actin genes form distinct clusters in the tree.

  16. Adaptive Evolution of Gene Expression in Drosophila

    Directory of Open Access Journals (Sweden)

    Armita Nourmohammad

    2017-08-01

    Full Text Available Gene expression levels are important quantitative traits that link genotypes to molecular functions and fitness. In Drosophila, population-genetic studies have revealed substantial adaptive evolution at the genomic level, but the evolutionary modes of gene expression remain controversial. Here, we present evidence that adaptation dominates the evolution of gene expression levels in flies. We show that 64% of the observed expression divergence across seven Drosophila species are adaptive changes driven by directional selection. Our results are derived from time-resolved data of gene expression divergence across a family of related species, using a probabilistic inference method for gene-specific selection. Adaptive gene expression is stronger in specific functional classes, including regulation, sensory perception, sexual behavior, and morphology. Moreover, we identify a large group of genes with sex-specific adaptation of expression, which predominantly occurs in males. Our analysis opens an avenue to map system-wide selection on molecular quantitative traits independently of their genetic basis.

  17. MRI Reporter Genes for Noninvasive Molecular Imaging

    Directory of Open Access Journals (Sweden)

    Caixia Yang

    2016-05-01

    Full Text Available Magnetic resonance imaging (MRI is one of the most important imaging technologies used in clinical diagnosis. Reporter genes for MRI can be applied to accurately track the delivery of cell in cell therapy, evaluate the therapy effect of gene delivery, and monitor tissue/cell-specific microenvironments. Commonly used reporter genes for MRI usually include genes encoding the enzyme (e.g., tyrosinase and β-galactosidase, the receptor on the cells (e.g., transferrin receptor, and endogenous reporter genes (e.g., ferritin reporter gene. However, low sensitivity limits the application of MRI and reporter gene-based multimodal imaging strategies are common including optical imaging and radionuclide imaging. These can significantly improve diagnostic efficiency and accelerate the development of new therapies.

  18. Apolipoprotein gene involved in lipid metabolism

    Science.gov (United States)

    Rubin, Edward; Pennacchio, Len A.

    2007-07-03

    Methods and materials for studying the effects of a newly identified human gene, APOAV, and the corresponding mouse gene apoAV. The sequences of the genes are given, and transgenic animals which either contain the gene or have the endogenous gene knocked out are described. In addition, single nucleotide polymorphisms (SNPs) in the gene are described and characterized. It is demonstrated that certain SNPs are associated with diseases involving lipids and triglycerides and other metabolic diseases. These SNPs may be used alone or with SNPs from other genes to study individual risk factors. Methods for intervention in lipid diseases, including the screening of drugs to treat lipid-related or diabetic diseases are also disclosed.

  19. Teilflächenspezifisches Kartoffelpflanzen durch eine GPS-gestützte Pflanzgutablage

    Directory of Open Access Journals (Sweden)

    Yves Reckleben

    2014-08-01

    Full Text Available Precision management makes it possible to align cultivation intensity with demand and thus adjust expenses to the necessary level. The proportion of normal-sized, marketable commodity – i. e. size grade 40 to 60 mm – can thus be increased in potato growing too. Planting distances adapted to the soil properties seem to achieve this objective. Yields could be improved further, especially in intensive potato-growing regions where irrigation and fertilizing already contribute to a consistently high yield potential in the rather light soils. Different planting distances on individual soil plots were tested in the authors’ own trials. Planting distances of 31.50 cm in light soils, 24.50 cm in medium soils and 27.50 cm in heavy soils produced the best results. Both the overall yield and the proportion of marketable goods were improved.

  20. Herausforderungen und Perspektiven zur Ermöglichung dualer Karrieren durch den Einsatz digitaler Medien

    Directory of Open Access Journals (Sweden)

    Linda Heise

    2018-03-01

    Full Text Available An important challenge of higher educational institutions is the inclusion of non-traditional learners. The following paper presents a project, which researched the challenges and special needs of student top athletes. First the paper will present the results of a qualitative study about the challenges and conditions of their study programs and commitments of student top athletes at the University of Applied Science Mittweida. Based on this results a blended learning scenario in combination with the flipped classroom has been introduced and tested.

  1. Communication men and waters: waters preservation by environmental education; Kommunikation Mensch und Gewaesser: Gewaesserschutz durch Umweltbildung

    Energy Technology Data Exchange (ETDEWEB)

    Link, F.G.; Hutter, C.P. [Akademie fuer Natur- und Umweltschutz beim Umweltministerium Baden-Wuerttemberg, Stuttgart (Germany)

    2007-07-01

    The successful execution of the legal regulation for preservation and development of waters depends above all on the awareness for environment. In times of increasing estrangement from nature it is necessary to prepare children for their works as conscious citizen, councilmen, mayors or future managers as early as their preschool environmental education. (orig.)

  2. Ultrasonic assisted fluxless flame soldering and brazing of aluminum alloys; Flussmittelfreies Flammloeten von Aluminiumlegierungen durch Ultraschallunterstuetzung

    Energy Technology Data Exchange (ETDEWEB)

    Bach, F.W. [FORTIS, ISOT GmbH, Witten (Germany); Moehwald, K.; Hollaender, U.; Stoll, P. [FORTIS, Hannover Univ., Hannover (Germany)

    2004-07-01

    Fluxless flame soldering and brazing of different pipe joints made of aluminium alloys can be realized by charging the joining zone with high power ultrasonic during the current process. The fundamental requirement for a successful ultrasonic assisted fluxless flame soldering or brazing is to couple the ultrasonic directly to the molten solder via a sonotrode. In order to guarantee defined acoustic conditions, constant temperature gradients in the sonotrode are required. This can be achieved by adapting a water cooling at the sonotrode. The acoustic energy which is applied to the joining zone causes the development of cavitations and ''micro-streams'' in the molten brazing filler metal. These effects ensure the occurrence of wetting and diffusion processes in the joining zone. (orig.)

  3. Control of electric drives. Innovation with intelligence; Regelung elektrischer Antriebe. Innovation durch Intelligenz

    Energy Technology Data Exchange (ETDEWEB)

    Hofer, K. [Fachhochschule Bielefeld (Germany)

    1998-10-01

    After outlining the classic design methods with transfer functions and frequency characteristics, the book presents modern theories, e.g. controllability, observability, field orientation, and fuzzy control. Analogous variants with operational amplifiers and digital variants with microprocessors are discussed in detail. Sensorless drive control is another key issue, owing to the fact that this is a promising concept that might result in considerable competitive advantages for its users. There is a special section with English language terms to account for the increasing globalisation of the markets, as well as an appendix with test questions and exercises. (orig./GL) [Deutsch] Ziel ist es, dem Leser zunaechst die klassischen Entwurfsmethoden mit Uebertragungsfunktionen und Frequenzkennlinien nahezubringen, um dann darauf die modernen Theorien wie Steuerbarkeit, Beobachtbarkeit, Raumzeigerdarstellung, Feldorientierung und Fuzzy Control aufzubauen. Darueber hinaus wird auf die analoge Realisierung der entworfenen Reglerstrukturen mit Operationsverstaerkern sowie die digitale Variante mit Mikroprozessoren sehr ausfuehrlich eingegangen. Sensorlose Antriebsregelungen bilden einen Schwerpunkt dieses Buchs, da auf diesem Sektor ein riesiges Wachstumspotential der Antriebstechnik mit erheblichen Wettbewerbsvorteilen fuer den Anwender liegt. Wegen der zunehmenden Globalisierung der Maerkte fuer Elektroantriebe sind in einem Sonderkapitel die wichtigsten englischen Fachausdruecke aufgefuehrt. Des weiteren finden sich im Anhang zahlreiche Testfragen und Uebungsaufgaben zur Vertiefung des Stoffs. (orig./GL)

  4. Simulation of biogas production by adding biochar; Steigerung des Biogasertrages durch die Zugabe von Pflanzenkohle

    Energy Technology Data Exchange (ETDEWEB)

    Roedger, Jan-Markus; Ganagin, Waldemar; Krieg, Andreas; Roth, Christian; Loewen, Achim [HAWK Hildesheim/Holzminden/Goettingen, Goettingen (Germany). Fachgebiet Nachhaltige Energie- und Umwelttechnik (NEUTec)

    2013-09-15

    One instrument to withdraw carbon dioxide from the atmosphere is the so called Biochar. Through photosynthesis and a thermochemical biomass treatment (pyrolysis) the bound carbon will be stabilized and thereby stored permanently. The product (biochar) is intended to be mainly used as soil enhancer on arable land. Early analyses indicate no economic feasibility yet. Therefore new ways of utilization need to be identified along the agricultural value chain to generate additional earnings. One option is the application of biochar as an additive within the biogas process. Different international research institutes were able to measure an increase in biogas production due to the biochar addition. One reason might be accumulation of methanogenic bacteria as well as the increase of the surface area to improve the microbial functionality which leads to additional gas production. The study presented here investigated the optimal process stage within the fermentation process (main digester or secondary fermenter) to add the biochar and thereby improve the gas production. By adding biochar to the main digester an increase in gas production of approximately 9 % over a time span of 91 days was measured. To analyse the effect within the secondary fermenter two different approaches were tested. The effect of the surface area was assessed by comparing the influence of original biochar and large char particles. The original Biochar led to an increase of more than 13 % and the large particles even enhanced methane production by 24 % over 91 days. (orig.)

  5. Modulation der Tumorreaktivität humaner gd T Lymphozyten durch Toll-like Rezeptor Agonisten

    OpenAIRE

    Shojaei, Amir Hamed

    2017-01-01

    TLR3 und -7 Liganden führten zu einer gesteigerten gd T-Zell-vermittelten Tumorzell-Lyse und einer…. ….verstärkte Produktion von Granzym A und -B Poly(I:C) verstärkt die Expression von CD54 auf Tumorzellen Imiquimod führt zur Runterregulation von HLA-Klasse I Molekülen in Tumorzellen und möglicherweise zu einer verminderten Bindungsaffinität des KIR NKG2A auf gd T-Zellen

  6. Multifunctional facades - design optimisation with CAD; Multifunktionale Fassaden - Effektive Auslegung durch CAD-Einsatz

    Energy Technology Data Exchange (ETDEWEB)

    Viotto, M. [Institut fuer Solare Energieversorgungstechnik (ISET), Kassel (Germany). Abt. fuer Anlagen- und Messtechnik

    1998-02-01

    The energetically optimal design of PV facades requires the consideration of many technical conditions (different elevations/orientations of modules; shading possibility; high and different module temperatures). It also requires the interface with architecture, civil and electrical engineering. To support this planning process, the software program PV-CAD was developed which rapidly calculates energy yield under conditions of inhomogeneous irradiance, shading, thermal behaviour of modules and electrical layout. PV-CAD is compatible with other civil and electrical engineering CAD programs and incorporates extensive databases (weather data; modules and inverter types). (orig.) [Deutsch] Fuer optimalen Energieertrag sind zahlreiche technische Randbedingungen bei der Auslegung einer Photovoltaik (PV)-Fassade zu beruecksichtigen (unterschiedliche Ausrichtungen/Neigungen der Module; moegliche Teilabschattung; hoehere und unterschiedliche Betriebstemperaturen). Installation und Betrieb einer PV-Fassade erfordern die Abstimmung zwischen Architektur, Bauwesen und Elektrotechnik. Zur Unterstuetzung der Planer wurde das Programm PV-CAD entwickelt, das den Energieertrag unter Beruecksichtigung von inhomogener Einstrahlung und Abschattung, Temperaturverhalten der Fassadenmodule sowie unterschiedlichen elektrischen Anlagenkonzepten schnell ermittelt; es fuegt sich nahtlos in CAD-Arbeitsumgebungen ein. (orig.)

  7. Review Essay: Dialogische Introspektion: Eine Erweiterung des empirischen Methodenspektrums durch die Wiederentdeckung von Introspektionsverfahren

    OpenAIRE

    Weggen, Jenny

    2011-01-01

    Das Buch "Dialogische Introspektion. Ein gruppengestütztes Verfahren zur Erforschung des Erlebens" beschreibt die Wiederentdeckung und Weiterentwicklung eines weitgehend in Vergessenheit geratenen qualitativen Verfahrens im Methodenspektrum von Psychologie und Sozialwissenschaften. Die Hamburger Forschungswerkstatt um Thomas BURKART, Gerhard KLEINING und Harald WITT greift das Verfahren der Introspektion auf, systematisiert es und entwickelt es unter Bezugnahme auf neue Erkenntnisse der empir...

  8. Review Essay: Dialogische Introspektion : eine Erweiterung des empirischen Methodenspektrums durch die Wiederentdeckung von Introspektionsverfahren

    OpenAIRE

    Weggen, Jenny

    2012-01-01

    Das Buch "Dialogische Introspektion. Ein gruppengestütztes Verfahren zur Erforschung des Erlebens" beschreibt die Wiederentdeckung und Weiterentwicklung eines weitgehend in Vergessenheit geratenen qualitativen Verfahrens im Methodenspektrum von Psychologie und Sozialwissenschaften. Die Hamburger Forschungswerkstatt um Thomas BURKART, Gerhard KLEINING und Harald WITT greift das Verfahren der Introspektion auf, systematisiert es und entwickelt es unter Bezugnahme auf neue Erkenntnisse der empir...

  9. Begnadigungen durch die tschechischen Präsidenten - Inspiration in der Geschichte?

    Czech Academy of Sciences Publication Activity Database

    Lojek, Antonín; Adamová, K.

    2014-01-01

    Roč. 4, č. 1 (2014), s. 5-13. ISBN 978-3-7001-7698-5. ISSN 2221-8890 Institutional support: RVO:68378122 Keywords : mercyof the President * constitution * countersignature Subject RIV: AG - Legal Sciences

  10. Beeinflussung des Angebots im Lebensmitteleinzelhandel durch Konsumenteninformationen: Eine Anwendung auf Basis der Theory of Planned Behavior

    OpenAIRE

    Mecking, Rebecca-Ariane

    2015-01-01

    Von Informationen, die Konsumenten z. B. über Kundenkarten dem Lebensmitteleinzelhandel (LEH) mitteilen, können auch sie selber profitieren, indem das Angebot besser an ihre Bedürfnisse angepasst wird. Basierend auf der Theory of Planned Behavior untersucht diese Arbeit, welche Faktoren entscheidend dafür sind, ob ein Konsument dem LEH zum Zwecke der Einflussnahme Informationen mitteilen möchte. Wichtig sind das Bewusstsein um diese Chance und ihre positiven Folgen. Sorgen um die Privatsphäre...

  11. Transparenz in der Fleischerzeugung – Wahrnehmung durch den Verbraucher am Point of Sale

    OpenAIRE

    Ludwig Arens; Mark Diemel

    2011-01-01

    Das gesellschaftliche Interesse an mehr Transparenz im Lebensmittelbereich ist erheblich gestiegen. Die jüngste Dioxin-Krise in Deutschland hat die vielfach erhobenen Forderungen nach einer gläsernen Produktion und mehr Informationen zur Lebensmittelsicherheit nochmals lauter werden lassen, ohne dass bereits hinreichend geklärt wäre, welche Informationen zum Produkt und dessen Herstellung und somit welcher Grad an Transparenz tatsächlich von Konsumenten beim Einkauf gefordert wird und verarbe...

  12. No childhood leukemia by emissions from nuclear installations; Keine Kinderleukaemien durch Emissionen aus Kernanlagen

    Energy Technology Data Exchange (ETDEWEB)

    Niggli, Felix [Kinderspital Zuerich (Switzerland). Klinik fuer Onkologie; Zuerich Univ. (Switzerland). Medizinische Fakultaet; Voelkle, Hansruedi [Fribourg Univ. (Switzerland). Physikdept.; Schaedelin, Juerg

    2017-10-01

    Childhood leukemia in the vicinity of nuclear power plants has long been a hot discussion topic. For some, it was proved that the nuclear power stations are responsible, for others it could not be explained by the established dosimetric models. Today, with some years of distance, and after a thorough review by a renowned English expert team, the COMARE working group, there is now more clarity. The 17{sup th} report of this working group published in 2016 confirms the hypothesis that there is no causal link between childhood leukemia and the radioactivity released by nuclear plants in the UK. It is therefore necessary to look for other explanations for dose cases where a significant increase has been observed. The commission also does not see any reason to question the established principles and methods of dosimetry and radiation risk assessment.

  13. Wie man Elementarteilchen entdeckt vom Zyklotron zum LHC : ein Streifzug durch die Welt der Teilchenbeschleuniger

    CERN Document Server

    Freytag, Carl

    2016-01-01

    Dieses Buch erklärt die physikalischen Grundlagen und die Technologien der Elementarteilchenforschung und beschreibt allgemeinverständlich Teilchenbeschleuniger und -detektoren sowie ihr Zusammenspiel. An einigen Meilensteinen der Forschung – von der Erzeugung von Transuranen über die Entdeckung exotischer Mesonen bis zum Higgs-Boson – zeigen die Autoren den Weg von der Theorie über das Experiment zum Forschungsergebnis auf. Gravitonen, Higgs-Teilchen, Neutrinos und Quarks – das Interesse an den kleinsten uns bekannten Teilchen ist seit Jahrzehnten ungebrochen und rückt damit auch die Laboratorien in den Blick, die an die Grenzen der Physik vorstoßen: Neben dem größten Experimentierfeld, das wir haben – dem Universum selbst - sind es die gigantischen Maschinen der Elementarteilchenphysik in Großforschungseinrichtungen wie dem CERN und dem DESY. Mit ihnen versuchen Forscher weltweit unter Einsatz extrem hoher Energien Zustände zu simulieren, wie sie zum Beginn unseres Universums kurz nach d...

  14. Förderung beruflicher Handlungskompetenz durch reflektiertes Erfahrungslernen mit digitalen Medien

    Directory of Open Access Journals (Sweden)

    Jan Hellriegel

    2017-02-01

    Full Text Available Eine gelingende Entwicklung beruflicher Handlungsfähigkeit in der dualen Berufsausbildung ist auf eine Kooperation von Lehrenden, Ausbildenden und Auszubildenden angewiesen. Häufig scheitert diese Zusammenarbeit jedoch an den spezifischen Lernkulturen und der räumlichen Trennung der verschiedenen Lernorte. Das BMBF-geförderte Forschungsprojekt KOLA: «Kompetenzorientiertes Lernen im Arbeitsprozess mit digitalen Medien» greift diese Problematik auf und verfolgt das Ziel, mithilfe digitaler Medien eine Lernortkooperation zu befördern. Erprobt wird das entwickelte System, bestehend aus einer App und einer Browser-basierten Plattform, in saarländischen Ausbildungsstätten des Elektrohandwerks im Untersuchungszeitraum von Oktober 2014 bis September 2017. Im Rahmen dieses Projektes wird ein lerntheoretischer Ansatz verfolgt, der sich im Kontext von Konstruktivismus und Pragmatismus verorten lässt. Der vorliegende Beitrag fokussiert dabei das didaktische Konzept des Projektes, welches einerseits die didaktischen Grundannahmen und andererseits das hieraus abgeleitete Nutzungsszenario darstellt.

  15. ICs mit aluminiumgebondeten Substraten sowie Verfahren zu deren Herstellung durch Direktkontaktierung mittels Ultraschall

    OpenAIRE

    Reinert, W.

    2007-01-01

    DE 102006003835 A1 UPAB: 20070402 NOVELTY - A method for fabricating a semiconductor module with a contacted active structure involves preparing a semiconductor wafer, locating at least one thin aluminum-contact elevation into conducting connection with the one of the metallic contacts and aligning the surface of the wafer parallel to and towards the upper face of a substrate having a metallic structure, and pressing the wafer on to the substrate and welding the wafer on to the substrate by u...

  16. Zweiwegintegration durch zweisprachige Bildung? Ergebnisse aus der Staatlichen Europa-Schule Berlin

    Science.gov (United States)

    Meier, Gabriela

    2012-06-01

    While there is no simple recipe of how to respond to the multitude of languages present in many European schools, this article presents a promising alternative to monolingual education. The focus is on Staatliche Europa-Schule Berlin (SESB), a two-way immersion (TWI) model that unites children whose mother tongue is German with children whose mother tongue is another locally spoken language in one class and teaches them together in two languages. Thus in this model, offered by 17 primary schools and 13 secondary schools in Berlin, pupils learn in two languages from and with each other. Based on a largely quantitative, quasi-experimental study with 603 students, evidence is provided that there are a number of peace-linguistic benefits that can promote two-way social integration, besides fostering personal and societal multilingualism. This suggests that TWI education as practised in Berlin could serve as an educational model for other multilingual parts of Europe.

  17. Mastoidberstung durch Schussverletzung - Therapie der traumatischen peripheren N. facialis-Läsion

    OpenAIRE

    Kostka, E; Koscielny, S; Wittekindt, C; Orlando, GL

    2007-01-01

    Entscheidend für die Prognose der traumatischen N. facialis-Läsion sind der Zeitpunkt des Auftretens nach der Schussverletzung, die Kenntnis über Schwere der Schädigung, die Beherrschung der operativen Erstversorgung sowie eine optimale Langzeittherapie.Das Geschoss zerschmetterte das Mastoid der 23-jährigen Patientin, zerstörte den N.facialis im mastoidalen Segment, passierte die A. carotis interna und Wirbelsäule und kam im Oropharynx zu liegen. Innerhalb von 24 Stunden erfolgte die Geschos...

  18. Aufbau durch Wasserstoffbrücken zusammengehaltener Nanostrukturen aus 15 Komponenten

    NARCIS (Netherlands)

    Jolliffe, K.A.; Timmerman, P.; Reinhoudt, David

    1999-01-01

    Insgesamt 72 Wasserstoffbrücken werden bei der spontanen Assoziation von Tetramelamin-Calix[4]arenen und Barbitursäurederivaten zu Nanoaggregaten des schematisch dargestellten Typs gebildet. Diese bestehen aus 15 Komponenten, die vollkommen diastereoselektiv assoziieren: Von acht möglichen

  19. Pulvermetallurgisch durch Reaktionssintern hergestelltes Bauteil aus einem Titanaluminid-Werkstoff und Verfahren zu seiner Herstellung

    OpenAIRE

    Kieback, B.; Boehm, A.; Weißgärber, T.

    2003-01-01

    DE 10228924 C UPAB: 20031216 NOVELTY - Component made from a titanium aluminide material produced by reaction sintering in a powder metallurgical method has oxygen as oxide of a further element formed by thermal treatment and/or during sintering embedded in the titanium aluminide material. The whole titanium aluminide material contains more than 0.1 wt.% oxygen. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a process for the powder metallurgy production of the above compone...

  20. "Neue Liebe, neues Leben" - Ein Goethe-Gedicht in der Vertonung durch Ludwig van Beethoven

    Directory of Open Access Journals (Sweden)

    Körndle, Franz

    2001-12-01

    Full Text Available Ludwig van Beethoven composed two versions of Goethe's poem „Neue Liebe, neues Leben" in 1798/99 and 1810 respectively. A comparison of both versions reveals a change in the composer's intention. At first in several passages he followed the more traditional technique of transferring single words of the text directly into musical figures. Later on he seemed more intent on expressing the general mood and eliminated some of the rather simplistic details in favour of a more subjective rendering of the poems content.

    [de] Ludwig van Beethoven vertonte das Goethe-Gedicht „Neue Liebe, neues Leben" in zwei Fassungen 1798/99 und 1810. Der Vergleich der beiden Fassungen ergibt, daß sich in der Zeit zwischen diesen beiden Fassungen die Vorstellungen des Komponisten von der musikalischen Umsetzung teilweise gewandelt haben müssen. Zunächst scheint Beethoven eher nach der traditionellen Méthode einer direkten Übertragung von Begriffen aus dem Text komponiert zu haben. Später war er dann aber eher an einer subjektiven Darstellung des Inhalts interessiert und drângte drängte zu einfach gerate Einzelheiten zugunsten der - nunmehr freilich recht differenziert gestalteten - Gesamtstimmung zurück.

  1. Vergleich der Exposition der Bevölkerung durch digitale und analoge Rundfunksender

    Directory of Open Access Journals (Sweden)

    M. Schubert

    2007-06-01

    Full Text Available Dieser Beitrag fasst die Ergebnisse eines Projektes zusammen, das vom Bundesamt für Strahlenschutz und dem Ministerium für Umwelt, Naturschutz und Reaktorsicherheit (BMU im Rahmen des Deutschen Mobilfunk Forschungsprogramms (DMF gefördert wurde. Hintergrund der Studie ist die Untersuchung der Auswirkung der Umstellung von der analogen auf die digitale terrestrische Rundfunkübertragung in Deutschland auf die Exposition der Bevölkerung.

  2. Abstrahierendes Lernen durch aktive Modellbildung: Evaluation eines Prozesses und einer Lernumgebung

    Directory of Open Access Journals (Sweden)

    Till Schümmer

    2015-11-01

    Full Text Available Experts and novices typically show different competencies in learning by abstraction. While novices typically consider the concrete example, experts often abstract from an example towards the underlying principles. This article presents a process and a computer-supported learning environment for learning through abstraction. An evaluation of the learning environment shows that pilot students were able to construct an abstract model from experience reports using the learning environment. The fewer common surface features the examples had, the easier was the abstraction. We also found that learners reflect on their learning process and adjusted the process to their specific learning preferences by following a modified process for learning through abstraction.

  3. Kontrolle des Wachstums HPV-positiver Zerviskarzinomzellen durch virale und zelluläre Proteine

    OpenAIRE

    Hurst, José

    2013-01-01

    Infektionen mit bestimmten HPV Typen wie HPV16 oder 18 können zur Entstehung von Karzinomen des Gebärmutterhalses (Zervixkarzinom, CxCa) führen. Interessanterweise ist für das Wachstum von CxCa-Zellen eine kontinuierliche Expression der HPV E6 und E7 Onkoproteine notwendig. E6 und E7 inaktivieren Zellzykluskontrollpunkte und verhindern die Induktion von Apoptose. Daher werden seit längerem unterschiedliche Möglichkeiten intensiv erforscht, die Expression der Onkoproteine in Karzinomzellen zu ...

  4. Our concept of the world is changed by nuclear power. Veraenderung unseres Weltbildes durch die Kernenergie

    Energy Technology Data Exchange (ETDEWEB)

    Knizia, K. (Vereinigte Elektrizitaetswerke Westfalen AG (VEW), Dortmund (Germany). Vorstand)

    1993-01-01

    In the absence of a positive agreement on the use of nuclear power mankind will not be able to live in peace. Viability is endagered by worldwide battles for energy. In the long term, the peaceful utilization of nuclear power must be given priority because the reserves of fossil energy resources will have been consumed in a few generations' time and renewable energy sources do not produce a yield high enough to meet the demand for energy of a continuously increasing world population. Creativity and captial must ensure sufficient and sustainable supplies of energy and goods for everybody. Environmental protection is facilitated by the use of nuclear power, for nuclear power is a source of energy whose waste can be removed from the biosphere completely and safely. (orig.).

  5. Signatures of dynamics in charge transport through organic molecules; Dynamisches Verhalten beim Ladungstransport durch organische Molekuele

    Energy Technology Data Exchange (ETDEWEB)

    Secker, Daniel

    2008-06-03

    The aim of the thesis at hand was to investigate dynamical behaviour in charge transport through organic molecules experimentally with the help of the mechanically controlled break junction (MCBJ) technique. the thesis concentrates on the complex interaction between the molecular contact configuration and the electronic structure. it is shown that by variation of the electrode distance and so by a manipulation of the molecule and contact configuration the electronic structure as well as the coupling between the molecule and the electrodes is affected. The latter statement is an additional hint how closely I-V-characteristics depend on the molecular contact configuration. Depending on the applied voltage and so the electric field there are two different configurations preferred by the molecular contact. A potential barrier between these two states is the origin of the hysteresis. A central part of the thesis is dealing with measurements of the current noise. Finally it can be concluded that the detailed discussion reveals the strong effect of dynamical interactions between the atomic configuration of the molecular contact and the electronic structure on the charge transport in single molecule junctions. (orig.)

  6. Optimum heating through self-learning methods. Optimales Heizen durch selbstlernende Verfahren

    Energy Technology Data Exchange (ETDEWEB)

    Giger, A. (Siemens-Albis AG, Zurich (Switzerland))

    1991-10-01

    In order of optimally heat a building, the required comfort conditions have to be fulfilled at the right time with possibly low energy expenditure. Control and regulation systems contribute essentially to this requirement. By means of stored program control with integrated direct digital control (SPS/DDC), complex control and regulation functions can be rather easily solved via the software. Data and conditions of different system components are interchanged or are monitored and changed if necessary through the operator by means of a service and observation system. This high capacity range of SPS/DDC facilitates the realization of functions which correspond to the latest trends of energy experts. (orig./BWI).

  7. Plant conditioning of aquatic sediments contaminated by heavy metals; Konditionierung Schwermetall belasteter Gewaessersedimente durch Pflanzen

    Energy Technology Data Exchange (ETDEWEB)

    Zehnsdorf, A.; Hoffmann, P.; Seidel, H. [UFZ - Umweltforschungszentrum Leipzig-Halle GmbH, Leipzig (Germany). Sektion Sanierungsforschung; Loeser, C. [Bauer und Mourik Umwelttechnik GmbH und Co., Schrobenhausen (Germany)

    2001-07-01

    When dredged sediments contaminated by heavy metals are exposed to air, the metals become partly soluble and the material turns into an environmental risk. As an alternative to disposal of dredged sludge in landfills, a cleansing process based on solid-bed bioleaching is under development. Solid-bed leaching works only on materials easily permeable by both air and water. Unfortunately, freshly dredged sediment is impermeable and thus unsuitable for solid-bed leaching. Therefore, the structure of the contaminated sediment must be altered in a preliminary conditioning step. The SECON plant, constructed for practical-scale conditioning experiments, can accommodate sediment packages of up to 2 m in thickness. Four treatment basins were each filled with 44 m{sup 3} of freshly dredged, silty sediments from the Weisse Elster River. One basin was planted with reed (Phragmites australis), a second one with reed canary grass (Phalaris arundinacea). The sediment in the third basin was exposed to spontaneously developing plants while the sediment in the fourth basin was kept free of vegetation. During one vegetation period, large amounts of water were extracted by evapotranspiration. As a result, cracks and cavities developed within the package through which air penetrated and oxidized the sediment. Plant root and microbe activities transformed the sludge into a crumbly, soil-like material. The transformation did not encompass the entire sediment package; after six months of treatment, a subsurface layer of various thickness remained anoxic with a muddy-pasty texture. Reed canary grass was best suited for conditioning. It structurally improved most of the sediment package and, in contrast to reed, it did not become infected with pests and diseases. (orig.)

  8. Fulmar Litter EcoQO monitoring along Durch and North Sea coasts

    NARCIS (Netherlands)

    Franeker, van J.A.

    2011-01-01

    Wastes from ships are an important source of litter in the marine environment in the Southern North Sea and cause serious economic and ecological damage. Inadequacies in the ship to shore waste delivery procedures are considered a major factor in illegal discharges. The European Union addressed the

  9. Renewable energies supported by GIS and land management; Erneuerbare Energien unterstuetzt durch GIS und Landmanagement

    Energy Technology Data Exchange (ETDEWEB)

    Klaerle, Martina (ed.)

    2012-11-01

    The author of the book under consideration reports on the support of renewable energy sources by Geographical Information Systems (GIS) and land management. The first part of this book is addressed to actual land political and planning legal fundamentals of the energy policy turnaround as well as on future developments of the planning instruments. The second part of this book presents GIS based tools and concrete application examples which are very valuable for regional authorities in the implementation of the energy policy turnaround: solar plant cadastre, holistic potential analysis for all forms of renewable energy systems, visibility studies, flexible power grids and so forth.

  10. Higher efficiency with cylinder-bank comprehensive exhaust manifold; Effizienzsteigerung durch Zylinderbank-uebergreifenden Kruemmer

    Energy Technology Data Exchange (ETDEWEB)

    Diez, Rainer; Kornherr, Heinz; Pirntke, Frank; Schmidt, Juergen [Friedrich Boysen GmbH und Co. KG, Altensteig (Germany)

    2010-05-15

    In close interdisciplinary cooperation with BMW Group, Boysen has developed an air-gap-insulated exhaust manifold that encompasses both banks of the 4.4 l V8 spark-ignition twin turbo engine of the BMW X5 M and BMW X6 M. The manifold merges the exhaust gas flow from the cylinders of the left-hand and right-hand cylinder banks in opposing pairs, thus optimising gas exchange. Due to improvements in response, torque and power characteristics of the engine, the cylinder-bank comprehensive exhaust manifold helps achieve high fuel efficiency. (orig.)

  11. Computervermittelte Kommunikation in der Stadtplanung. Unterstützung formaler Beteiligungsverfahren durch Issue Based Information Systems

    OpenAIRE

    Märker, O.

    1999-01-01

    Computer-Mediated Communication in Urban Planning - Supporting Formal Participation Processes with Issue Based In formation Systems A "new planning culture" is increasingly practiced in urban planning. New forms of participation initiate and further communication and discussion processes between different interest groups. This reorientation in the participation practice of urban planning also takes place in discourses of planning theory in which "communication planning" or "new planning cultu...

  12. Elektronische Marktwirtschaft?: Grundlegende Veränderungen durch den Electronic Commerce

    OpenAIRE

    Kaiser, Helmut

    2017-01-01

    Electronic commerce has become an important factor in present-day business. The reflection ofbusiness ethics asks four questions: - To what extent does e-commerce improve the ethical quality of decision making and performing in the sphere of commerce? - Does e-commerce promote the >>reason

  13. Practice orientated rehabilitation of a contaminated industrial area by immobilisation; Praxisgerechte Altlastensanierung durch Immobilisierung

    Energy Technology Data Exchange (ETDEWEB)

    Korte, K. [Wersche GmbH, Braunschweig (Germany); Schulz, H. [Wersche GmbH, Braunschweig (Germany)

    1996-12-31

    As the result of a selection of rehabilitation strategies for a heavy metal contaminated soil on a city railway construction site in Hannover an immobilising was recommended as an economical alternative which could be integrated into the planned contruction works without any problems. The immobilising is not an all-time strategy, but the existing ecological risk may be reduced by considering the site specific risk, the type of pollutants and landuse. The suitability of the immobilising must be calculated for every single case and a supervising and control of the fixed rehabilitation targets is essential. (orig.) [Deutsch] Fuer die Sanierung von schwermetallbelastetem Boden im Bereich einer Stadtbahn-Baustelle in Hannover wurde eine Verfahrensauswahl verschiedener Sicherungs- und Sanierungsmassnahmen durchgefuehrt. Als wirtschaftliche Alternative, die reibungslos in den Bauablauf integriert werden konnte, stellte sich im vorliegenden Fall die Immobilisierung und der Wiedereinbau des belasteten Materials heraus. Die Eignung des Verfahrens der Immobilisierung ist dabei fuer jeden Einzelfall unter Beruecksichtigung der spezifischen lokalen Randbedingungen wie z.B. Art und Bindung der Schadstoffe sowie bestehende und geplante Nutzung kritisch zu pruefen. Eine Ueberwachung der Verfahrensergebnisse und Kontrolle der Einhaltung von Sanierungszielen ist dabei unabdingbar. (orig.)

  14. Direkte und indirekte Effekte von Pilzen und Oomyceten auf die Laub Zersetzung durch aquatische Makroinvertebraten

    OpenAIRE

    Aßmann, Christine

    2010-01-01

    The decomposition processes in lakes are documented in few reports only, and it is therefore important to improve our understanding of the process of leaf conditioning and its effects on the benthic community in lakes. In the present thesis I investigated how the identity of microbial colonisers affects the consumption by macroinvertebrates organisms were examined. For the studies presented here the shredder Gammarus roeselii and small-particle-feeder Limnomysis benedeni, a recent invader of ...

  15. Spinal trauma: first aid from cross-sectional imaging; Trauma der Wirbelsaeule: erste Hilfe durch Schnittbildverfahren

    Energy Technology Data Exchange (ETDEWEB)

    Schueller, G.; Schueller-Weidekamm, C. [Emergency Radiology Schueller, Neerach (Switzerland)

    2014-09-15

    The diagnosis of the traumatized spine is one of the key issues for trauma radiologists. The cross-sectional imaging procedures, computed tomography (CT) and magnetic resonance imaging (MRI) are the essential methods in spinal trauma radiology. These modalities are of great help in accurately assessing injury patterns and extent and in providing indications of patient outcome. In contrast to cross-sectional imaging, radiography has a role in the evaluation of minor spinal trauma only. It is generally accepted that trauma radiologists do not use typical classifications to evaluate the spine partly because such an ideal classification system does not yet exist. Not least because of this classification difficulty, eponyms and synonyms are widely used to describe traumatology of the spine as a high level of specific information is included in these various terms. The members of the trauma team should be aware of the strengths and limitations of the methods used in the assessment of the spine. This article provides a brief outline of fundamental knowledge about the diagnosis of spinal trauma. (orig.) [German] Die Beurteilung der verletzten Wirbelsaeule nimmt fuer Traumaradiologen eine zentrale Stellung ein. Die Schnittbildverfahren CT und MRT sind ihre wesentlichen Arbeitsmethoden. Sie helfen dabei, schnell und mit hoher Zuverlaessigkeit Aussagen ueber Art und Ausmass von Verletzungen zu treffen sowie Hinweise auf die Prognose der Patienten zu geben. Die Projektionsradiographie hat ihre Bedeutung lediglich in der Diagnostik des Bagatelltraumas und ist in ihrer Aussagekraft auch dort nicht unumstritten. Traumaradiologen bedienen sich nicht ausschliesslich typischer Klassifikationen des Wirbelsaeulentraumas, z. T. auch deshalb, da es die ideale Klassifikation aus heutiger Sicht nicht gibt. Vielmehr ist es wichtig, auch ueber Eponyme und Synonyme Bescheid zu wissen, da sie ein hohes Mass an spezifischen Informationen der spinalen Verletzungen verinnerlichen. Alle Mitglieder eines Traumateams sollen sich der Staerken und Schwaechen der verwendeten radiologischen Techniken bei der Beurteilung des Wirbelsaeulentraumas bewusst sein. Dieser Artikel gibt einen kurzen Abriss ueber die wesentlichen diagnostischen Kenntnisse der traumatisierten Wirbelsaeule. (orig.)

  16. Aufklärung des Europäischen Forschungsraums durch Geschlechterwissen?

    Directory of Open Access Journals (Sweden)

    Heike Kahlert

    2008-07-01

    Full Text Available Zentrales Verdienst der vorliegenden empirischen Studie ist es, anschaulich das Netzwerk von Wissenschaftlerinnen, Politikerinnen sowie Mitarbeiterinnen und Mitarbeitern der nationalen und insbesondere der europäischen Forschungsverwaltung zu beschreiben, das ermöglichte, Gender Mainstreaming im Sechsten Forschungsrahmenprogramm der EU zu implementieren. Außerdem wird die Verknüpfung aus formellen und informellen Politikstrukturen und Genderexpertise beschrieben.

  17. Vom Terrorismus zum Wandel durch Annäherung: Houellebecqs ‚Soumission‘

    Directory of Open Access Journals (Sweden)

    Wolfgang Asholt

    2016-03-01

    Full Text Available Es handelt sich um den Text eines Vortrags beim von Ursula Hennigfeld und Elmar Schafroth organisierten Workshop „Zensur, Political Correctness und Terrorismus“  (Universität Düsseldorf, 30.6. – 2.7. 2015.

  18. Kosteneffiziente Honbearbeitung durch intelligente und flexible Verknüpfung von autarken Honzentren

    Science.gov (United States)

    Roth, Günther

    Die Planungsabteilungen in der Automobilindustrie werden vor immer größere Herausforderungen gestsellt. Der zur Verfügung stehende Zeitraum zu einer seriensicheren Umsetzung der entwicklungs- und produktionstechnischen Forderungen wird von Projekt zu Projekt kürzer. Aufgrund des hohen Wettbewerbsdrucks und daraus resultierenden erforderlichen Kostenoptimierungen, die schnelleren Timeto-market-und Innovationszyklen und die vermeintlich zur Kundenbindung erforderlichen, kaum mehr überschaubaren Diversifizierungen in den Modellpaletten lassen den Planungshorizont immer kürzer werden. Die zu beschaffenden Fertigungsanlagen sollen aber in den nächsten 8-12 Jahren all die Varianten produzieren können, die heute nur teilweise oder noch gar nicht bekannt sind.

  19. Optimization of energy input for massive forming manufacturing processes. Optimaler Energieeinsatz bei der Fertigung durch Massivumformung

    Energy Technology Data Exchange (ETDEWEB)

    Herlan, T. (Stuttgart Univ. (Germany, F.R.). Inst. fuer Umformtechnik)

    1989-01-01

    The specific end or primary energy consumption of single manufacturing stages is determined with the help of process chain analysis. The overall system considered is divided into process chain segments which themselves are represented in units of process stages. For the investigation of the energy used in single-piece manufacture by massive forming, there is a projected process stage which shows the energy consumption of the initial semi-finished product. The second process stage determines the energy used for manufacturing by means of massive forming. The materials examined are steel and aluminium materials from which workpieces are produced almost waste-free by means of cold, warm or hot extrusion. The required energy consumption as well as the manufacturing and energy costs for any manufacturing by means of massive forming are determined with the help of a computer program. From surveys of several manufacturing sequences it can be shown that 10-20% of the energy consumption can be saved under existing operational conditions, without investments having to be made. On a long-term basis, up to 40% of the energy consumption may be saved by adjusting the manufacturing equipment. (orig./MM).

  20. Der Erwerb der Verbflexion durch thailändische Immigrantinnen in der Schweiz. Eine Bestandaufnahme.

    Directory of Open Access Journals (Sweden)

    Attaviriyanupap, Korakoch

    2006-01-01

    Full Text Available This article focuses on the acquisition of German verb inflection by native speakers of Thai, an isolated language which has no concept of inflection at all. The acquisition process of German verb morphology is analyzed based on all the verb inflectional affixes found in the corpus consisting of spontaneous utterances in Standard German produced by16 female immigrants living in German-speaking Switzerland. It aims to find out a systematic acquisition order of verb inflectional morphemes and the explanation to this sequence, especially to answer the following four questions: 1 What is the acquisition order of verb inflectional morphemes found in this group of informants 2 Are there any differences between the acquisition of finite and that of non-finite verbs? 3 Are there any differences in the verb morphology acquisition of different types of verbs? 4 Does the acquisition of verb inflection by these informants share more similiarities with the instructed or with the natural acquisition of German as a second language?

  1. Vorrichtung zur Nachbehandlung von Abgas durch Kombination von Gasentladung und Katalysator

    OpenAIRE

    Luetkemeyer, G.; Neff, W.; Pochner, K.; Lepperhoff, G.

    1998-01-01

    The unit for after-treatment for waste or exhaust gases comprises a reactor chamber, through the length of which the gas flows, from inlet to outlet (61, 62). A first electrode (10) is located on or near a flat dielectric (20) screening it from the reaction chamber. The second electrode (32) is near the dielectric, within the reaction chamber, and is flat and perforated. The second electrode (32) is oppositely-connected to the first electrode. Also claimed is the method of gas after-treatment...

  2. Das Fremde als das Eigene : Entstehung der südkoreanischen Wirtschaftskultur durch fremdbestimmte Modernisierung

    OpenAIRE

    Yim, Il-Sop

    2014-01-01

    This thesis tries to search for historical origins of two salient features of South Korean economic culture: authoritarianism and particularism. Contrary to prevailing opinion that these features originated from Confucianism, which was the state ideology in the Chosun dynasty, this work contends that they are rather influenced by the way how the country was modernized under foreign influences since the end of 19th century. Social Darwinism, Japanese colonial rule and Korean War caused by sepa...

  3. Inhibition of hydrate formation by kinetic inhibitors. Literature study; Inhibierung von Erdgashydraten durch kinetische Inhibitoren. Literaturstudie

    Energy Technology Data Exchange (ETDEWEB)

    Eberhardt, E.; Meyn, V.; Rahimian, I. [Institut fuer Erdoel- und Erdgasforschung, Clausthal-Zellerfeld (Germany)

    2000-04-01

    The aim of this study was to represent the state-of-the art of the inhibition of gas hydrates. Corresponding to recent publications the kinetic inhibition was considered in particular. Special inhibitors were validated using a set of criteria derived from different experimental test methods. Best results were obtained by the application of terpolymer VC-713 especially in relation to nucleation and crystal growth, followed by PVCap (polyvinylcaprolactame) and THI (threshold hydrate inhibitor), the chemical structure of which is derived from the antifreeze glycopeptids of antarcitc winter flounder. (orig.) [German] Die vorliegende Literaturstudie gibt den derzeitigen Stand der Kenntnis zur Inhibierung von Gashydraten wieder. Entsprechend der neueren Literatur wird insbesondere auf die kinetische Inhibierung eingegangen. Zur Beurteilung der verschiedenen Inhibitoren werden Bewertungskriterien zur Validierung der mit unterschiedlichen Untersuchungsmethoden erzielten experimentellen Ergebnisse angegeben. Anhand dieser Vorgehensweise zeigte sich, dass mit dem Terpolymer VC-713 die besten Ergebnisse, insbesondere im Hinblick auf Keimbildung und Wachstum, erzielt werden konnten. Sehr gute Ergebnisse wurden auch mit dem Polyvinylcaprolactam (PVCap) und den aus den Antigefrierpeptiden der antarktischen Winterflunder abgeleiteten Threshold Hydrate Inhibitoren (THI) erhalten. (orig.)

  4. Aneignung und Interpretation der monadologischen Metaphysik von Leibniz durch die Metaphysik des Daseins

    Directory of Open Access Journals (Sweden)

    Hardy Neumann

    2014-06-01

    Full Text Available The research of H.-G. Gadamer and F. Volpi have showed the intimate connection between Sein und Zeit and the philosophy of Aristotle, specially the Nicomachean Ethics. The influence of other thinkers on the hermeneutical phenomenology has received less attention. Amongst the thinkers, whose contributions have not been sufficiently highlighted figures Leibniz. In this context, some relations between Leibniz’s monadological thinking and the metaphysics of Heidegger’s Dasein are considered and interpreted in this paper. It is argued that, to certain extent, an appropriation of Monadology’s metaphysics takes place in Dasein’s metaphysics.

  5. Videobasierte Rauchdetektion durch mehrfache Bewegungsschätzung und Anpassungen für geringe Rauchdichten

    OpenAIRE

    Stadler, Anton

    2017-01-01

    In dieser Arbeit wird untersucht, wie Rauch mit videobasierten Methoden erfasst werden kann. Die Bewegungsschätzung erweist sich als effektivstes Merkmal. Strömungsmessungen zeigen, dass das Aufstiegsverhalten von Rauch detailliert erfasst wird. Ein Rauchdetektionsverfahren wird vorgestellt und für geringe Bewegungsgeschwindigkeiten sowie für geringe Rauchdichten optimiert. Ein Verfahren mit mehrfacher Bewegungsschätzung stellt für verschiedene Entfernungen und Rauchdichten geeignete Konfigur...

  6. Decontamination of nuclear graphite by thermal processing; Dekontamination von Nukleargraphit durch thermische Behandlung

    Energy Technology Data Exchange (ETDEWEB)

    Florjan, Monika W.

    2010-04-15

    The main problem in view of the direct disposal of the nuclear graphite is its large volume. This waste contains long-lived and short-lived radionuclides which determine the waste strategy. The irradiated graphite possess high amount of the {sup 14}C isotope. The main object of the present work was the selective separation of {sup 14}C isotope from the isotope {sup 12}C by thermal treatment (pyrolysis, partial oxidation). A successful separation could reduce the radiotoxicity and offer a different disposal strategy. Three different graphite types were investigated. The samples originate from the reflector and from the flaking of spherical fuel elements of the high-temperature reactor (AVR) Juelich. The samples from the thermal column of the research reactor (Merlin, Juelich) were also investigated. The maximum tritium releases were obtained both in inert gas atmosphere (N{sub 2}) and under water vapour-oxidizing conditions at 1280 C and 900 C. Furthermore it could be shown that 28% of {sup 14}C could be released under inert gas conditions at a 1280 C. By additive of oxidizing agent such as water vapour and oxygen the {sup 14}C release could be increased. Under water vapour-oxidizing conditions at a temperature of 1280 C up to 93% of the {sup 14}C was separated from the graphite. The matrix corrosion of 5.4% was obtained. The selective separation of the {sup 14}C is possible, because a substantial part of the radiocarbon is bound near the grain boundary surfaces. (orig.)

  7. Bereitstellung von Primärregelleistung durch stationäre Großbatteriespeicher

    OpenAIRE

    Stenzel, Peter

    2016-01-01

    Die technologische Entwicklung und die rasch fallenden Systempreise führen dazu, dass Batterieenergiespeichersysteme für verschiedene stationäre Anwendungen zunehmend attraktiv werden.Die Fähigkeit, schnell und genau auf Frequenzabweichungen reagieren zu können ist ein Hauptmerkmal von Batteriespeichern, und macht sie zu idealen Kandidaten für die Bereitstellung von Primärregelleistung. Dies spiegelt sich in einer zunehmenden Anzahl an realisierten und sich in Planung befindlichen Batteriespe...

  8. Bone alterations by stress in athletes. Schaedigung des Knochens durch Ueberlastung bei Leistungssportlern

    Energy Technology Data Exchange (ETDEWEB)

    Doege, H. (Bezirkskrankenhaus ' Friedrich Wolf' , Abt. fuer Nuklearmedizin, Chemnitz (Germany))

    1990-01-01

    This report describes our experiences with the bone imaging in athletes. We studied 10 athletes and 10 other patients with spondylolisthesis of the lumbar spine and 16 athletes with suspicion of alterations of extremities. An increased uptake of this radiopharmaceutical was detected in six of 10 athletes with spondylolisthesis caused probably by stress fracture. Bone scans were negative in seven of 16 athletes with suspicion of lesion of extremities. In the remaining 9 patients scans were abnormal and showed periosteal injuries, epiphyseal alteration, joint abnormalities, tibial stress fractures and couvert fracture. It was also abnormal in bone injuries not evident in radiography. (orig.).

  9. Vorbereitung der medizinstudenten auf den übergang zur postgraduatiertenausbildung durch wahlfächerrotation

    NARCIS (Netherlands)

    van den Broek, W. E.Sjoukje; Wijnen-Meijer, Marjo; Ten Cate, Olle; van Dijk, Marijke

    2017-01-01

    Objectives: This study adds to the ongoing discussion on how to ease the transition from undergraduate medical training to postgraduate training. In the Netherlands there is no central matching system for admission to residency. Medical school graduates just apply for a position in an open job

  10. Broadband piezoelectric energy harvesting using nonlinear magnetic forces; Bandbreitensteigerung von piezoelektrischen Energy Harvesting Systemen durch Magnetkraefte

    Energy Technology Data Exchange (ETDEWEB)

    Westermann, Henrik; Neubauer, Marcus; Wallaschek, Joerg [Hannover Univ. (Germany). Inst. fuer Dynamik und Schwingungen

    2012-07-15

    Using ambient energy by piezoelectric energy harvesting systems received much attention over the last years. Most vibration-based generators produce a sufficient power only if the transducer is excited in its resonance frequency. The use of magnetic forces suggests a promising strategy to increase the efficiency. This paper presents different ways for broadband piezoelectric energy harvesting using nonlinear magnetic forces. (orig.)

  11. Pyritoxidationsprozesse im cretazischen Emschermergel infolge von aeroben Bedingungen durch den Emscherumbau

    Science.gov (United States)

    Droste, Björn; Wisotzky, Frank

    2015-09-01

    Due to the restoration of the Emscher river system, large amounts of the Emschermergel, a Cretaceous sediment containing significant amounts of carbonate and pyrite, will be released from its anoxic state. The oxidation of pyrite results in the release of protons, iron and sulfate. In order to evaluate the impact of the reaction products, extensive laboratory investigations were carried out. The methodology can detect pyrite content in the range of magnitude of the Rhenish lignite mining area (average 0.21 wt.% Pyritic-S). The formation of a naturally-occurring weathering horizon could also be detected. Under laboratory conditions, further oxidation of the weathered laboratory samples could be observed. Column experiments show significant release of sulfate at high concentrations. Hydrochemical modelling and acid-base accounting show neutralization of the free protons by the effective carbonate buffer in the sediment.

  12. Detection of ductile crack initiation by acoustic emission testing; Detektion von duktiler Rissinitiierung durch Schallemissionen

    Energy Technology Data Exchange (ETDEWEB)

    Richter, H.; Boehmert, J.; Viehrig, H.W. [Forschungszentrum Rossendorf e.V. (FZR) (Germany). Inst. fuer Sicherheitsforschung; Valo, M. [VTT Manufacturing Technology, Materials and Structural Integrity, Espoo (Finland)

    1998-08-01

    A Charpy impact test equipment is described permitting simultaneous measurement of impact force, crack tip opening, acoustic emissions and magnetic emissions. The core of the equipment is an inverted pendulum ram impact testing machine and the tests have been performed with laterally notched, pre-fatigue ISO-V specimens made of steels of various strength and tougness properties. The tests are intended to ascertain whether the acoustic emission method is suitable for detecting steady crack initiation in highly ductile steels. (orig./CB) [Deutsch] Es wird eine Kerbschlagbiegeanordnung vorgestellt, mit der simultan Schlagkraft, Rissoeffnung, Schallemission und magnetische Emission gemessen werden koennen. Grundbaustein der Anordnung ist ein invertiertes Pendelschlagwerk. Mit dieser Anordnung wurden seitgekerbte, vorermuedete ISO-V Proben aus Staehlen mit unterschiedlichen Festigkeits-Zaehigkeitseigenschaften geprueft. Die Untersuchung soll aufzeigen, ob Schallemission zur Detektion der stabilen Rissinitierung an hochzaehen Staehlen geeignet ist. (orig./CB)

  13. Empirische Ergebnisse zur Nutzung sozialer Netzwerkplattformen durch E-Learning-Akteurinnen und Akteure an Hochschulen

    Directory of Open Access Journals (Sweden)

    Philip Meyer

    2017-03-01

    Full Text Available Das Informationsportal «e-teaching.org» richtet sich an E-Learning-Akteure/-innen an Hochschulen, denen es Bildungsinhalte und aktuelle Informationen zu didaktischen, technologischen und organisatorischen Aspekten des Lernens mit digitalen Medien bietet. In den Monaten Juli und August 2014 nahmen 137 Nutzer/innen an einer halb-standardisierten, nicht-repräsentativen Online-Befragung teil, welche die Bedeutung sozialer Netzwerke für das Portal in Hinblick auf Austausch- und Informationsprozesse in beruflichen und privaten Kontexten erhob. Zudem wurde das Twitternetzwerk des Portals mit rund 40.000 Verbindungen zwischen 1.600 Personen (Stand: April 2014 anhand einer sozialen Netzwerkanalyse untersucht. Es deutet sich an, dass soziale Netzwerke zu bestimmten Zwecken professionell genutzt werden. Bei der Mehrzahl der Befragten ist dies auf Twitter die gegenseitige Vernetzung sowie die Informationsaufnahme und -streuung, wohingegen auf Facebook die Teilnahme und der soziale Austausch in Gruppen dominiert. Mit rund einem Drittel sieht allerdings ein nicht unerheblicher Teil der Befragten von einer beruflichen Beteiligung in sozialen Netzwerken ab und nutzt stattdessen lieber E-Mails, Blogs und RSS-Feeds.

  14. Biogenic methane from hydrothermal gasification of biomass; Biogenes Methan durch hydrothermale Vergasung von Biomasse

    Energy Technology Data Exchange (ETDEWEB)

    Schubert, M.; Vogel, F.

    2007-09-15

    This final report for the Swiss Federal Office of Energy (SFOE) reports on work done in the area of gasification of biomass. The use of dung, manure and sewage sludge as sources of energy is described and discussed. Hydrothermal gasification is proposed as an alternative to conventional gas-phase processes. The aim of the project in this respect is discussed. Here, a catalytic process that demonstrates the gasification of wet biomass to synthetic natural gas (SNG) in a continuously operating plant on a laboratory scale is being looked at. Difficulties encountered in preliminary tests are discussed. Long-term catalyst stability and the installations for the demonstration of the process are discussed, and gasification tests with ethanol are commented on.

  15. Der Bruch der Theorie in der Praxis durch Not. Bemerkungen zu Lujo Brentanos Sicht von Arbeitsmarktproblemen

    OpenAIRE

    Schlicht, Ekkehart

    2005-01-01

    Some Remarks on Lujo Brentano's View of Labor Market Problems Lujo Brentano (1844-1931) aimed for "realism" in economics. Regarding labor market theory, two topics are of particular interest: His investigations on "Hours and Wages in Relation to Production" (Scribner's 1894) and his analysis "On the History and Development of Gilds and the Origins of Trade Unions" (Trubner 1870). The topics of are discussed with a view on recent developments in labor economics.

  16. Zugang zu neuen FuE-Konzepten durch innovative Verfahren der Pflanzenphänotypisierung

    Directory of Open Access Journals (Sweden)

    Altmann, Thomas

    2011-10-01

    Full Text Available The development and use of automated procedures of non- or minimal invasive monitoring of plant traits and features opens new opportunities for the identification of genetic factors determining plant characteristics and performance parameters, for the investigation of environmental effects on trait expression, for the selection of individuals or lines with preferred features from large populations, for the optimization of plant production systems, and for the recognition of novel uses of plants. This is achieved through development and application of precise and unbiased measurement procedures (not influenced by human perception, which (ideally are amenable to high-throughput approaches. They enable more accurate and much larger scale investigations or screenings than hitherto possible, they allow the detection of features that previously could not be assessed, and they offer access to monitoring and quantification of dynamic processes through repeated measurements over time. To this end, (novel sensor and detection systems have to be developed and an implemented, automation procedures have to be adapted and established, corresponding facilities have to be constructed and installed, which support monitoring and/or simulation of certain environmental parameters (in addition to the measurement of plant features and effective data analysis tools have to be developed and implemented.

  17. Verbesserung der seismischen Kapazität von Mauerwerkswänden durch Verwendung von Elastomerlagern

    OpenAIRE

    Aldoghaim, Eyad

    2011-01-01

    Ein großer Teil der Schäden wie auch der Verluste an Gesundheit und Leben im Erdbebenfall hat mit dem frühzeitigen Versagen von Mauerwerksbauten zu tun. Unbewehrtes Mauerwerk, wie es in vielen Ländern üblich ist, weist naturgemäß einen begrenzten Erdbebenwiderstand auf, da Zugspannungen und Zugkräfte nicht wie bei Stahlbeton- oder Stahlbauten aufgenommen werden können. Aus diesem Grund wurde bereits mit verschiedenen Methoden versucht, die Tragfähigkeit von Mauerwerk im Erdbebenfall zu ve...

  18. Von Whorf zu Malotki - Eine Reise durch Hopi-Raum und Hopi-Zeit

    DEFF Research Database (Denmark)

    Robering, Klaus

    2014-01-01

    how the Hopi language represents temporal and spatial matters. The Hopi conception of space and time (as conceived by Whorf) is contrasted with the views of classical and relativistic physics. The third part is devoted to the reception of Whorf’s views in German linguistics in the 1960s. A brief......The article investigates the views of Whorf on the linguistic representation of space and time and their reception in German linguistics in the 1960s. Its first part summarizes both Whorf’s general conception of the relationship between language and cognition and his semantic analysis of the way...... discussion of classical German philosophy of language (Hamann, Herder, Humboldt) prepares the stage for relating Whorf’s views to those of Leo Weisgerber’s ‘Inhaltbezogene Grammatik’, then the dominant trend in German linguistics. The studies of ‘Hopi space’ and ‘Hopi time’, by Ekkehart Malotki (a disciple...

  19. Institutionalisierung durch Imitation? Die Europäisierung des Parlamentarismus in Mittel- und Osteuropa / Aron Buzogany

    Index Scriptorium Estoniae

    Buzogany, Aron

    2015-01-01

    Artikli teemaks on parlamentarismi euroopastumine Kesk- ja Ida-Euroopa riikides (sh Eesti), püüdes leida vastus küsimusele, kuidas on rahvusriikide parlamendid suutnud institutsionaalselt euroopastumise väljakutsele vastata?

  20. Climate protection by reducing cooling demands in buildings; Klimaschutz durch Reduzierung des Energiebedarfs fuer Gebaeudekuehlung

    Energy Technology Data Exchange (ETDEWEB)

    Bettgenhaeuser, Kjell; Boermans, Thomas; Offermann, Markus; Krechting, Anja; Becker, Daniel [Ecofys Germany GmbH, Koeln (Germany)

    2011-06-15

    The aim of this study is to conduct estimation on the potential reduction in electricity demand from cooling appliances in buildings in Germany. Current electricity demand and greenhouse-gas emissions will be investigated through desk research for residential and non-residential buildings. Based on building simulations, conventional, alternative and renewable technologies will be compared for different reference buildings. An economic and environmental assessment will evaluate the technologies per reference building in further detail. The main result will be an estimation of the potential energy demand reduction for the alternative/ regenerative technologies in the building stock. This will be based on the conditioned floor area and retrofit rates per system. Furthermore, the influence of cooling in buildings on energy demand will be annotated. Barriers in the reduction of energy demand will be described possible actions will be discussed along with types of policy instruments and consumer information. (orig.)

  1. Visualisierung analoger Schaltungen durch 3-D Animation von transienten SPICE-Simulationen

    Directory of Open Access Journals (Sweden)

    J. Becker

    2007-06-01

    Full Text Available Für das Zeichnen analoger Schaltpläne wird oft versucht, die Potentialverteilung in der entsprechenden Schaltung auszunutzen, um eine Platzierung der Bauteile nach abfallendem Potential vorzunehmen. Mit Hilfe von Computerunterstützung gelingt es, eine verallgemeinerte dreidimensionale Platzierungsstrategie anzuwenden, die allein auf Grund der Potentialwerte einer Schaltung die automatische Generierung einer technisch exakten Potentialdarstellung erlaubt. Somit ist es möglich, die Ergebnisse von transienten SPICE-Simulationen in jedem Zeitschritt darzustellen und eine Animation des zeitlichen Verhaltens zu erzeugen. Die Umsetzung dieser Methode zur Einbettung in eine webbasierte Lern – und Arbeitsplattform wird im Folgenden erläutert.

  2. Cancer Genes in Lung Cancer

    Science.gov (United States)

    El-Telbany, Ahmed

    2012-01-01

    Cancer is now known as a disease of genomic alterations. Mutational analysis and genomics profiling in recent years have advanced the field of lung cancer genetics/genomics significantly. It is becoming more accepted now that the identification of genomic alterations in lung cancer can impact therapeutics, especially when the alterations represent “oncogenic drivers” in the processes of tumorigenesis and progression. In this review, we will highlight the key driver oncogenic gene mutations and fusions identified in lung cancer. The review will summarize and report the available demographic and clinicopathological data as well as molecular details behind various lung cancer gene alterations in the context of race. We hope to shed some light into the disparities in the incidence of various genetic mutations among lung cancer patients of different racial backgrounds. As molecularly targeted therapy continues to advance in lung cancer, racial differences in specific genetic/genomic alterations can have an important impact in the choices of therapeutics and in our understanding of the drug sensitivity/resistance profile. The most relevant genes in lung cancer described in this review include the following: EGFR, KRAS, MET, LKB1, BRAF, PIK3CA, ALK, RET, and ROS1. Commonly identified genetic/genomic alterations such as missense or nonsense mutations, small insertions or deletions, alternative splicing, and chromosomal fusion rearrangements were discussed. Relevance in current targeted therapeutic drugs was mentioned when appropriate. We also highlighted various targeted therapeutics that are currently under clinical development, such as the MET inhibitors and antibodies. With the advent of next-generation sequencing, the landscape of genomic alterations in lung cancer is expected to be much transformed and detailed in upcoming years. These genomic landscape differences in the context of racial disparities should be emphasized both in tumorigenesis and in drug

  3. Gene therapy for prostate cancer.

    LENUS (Irish Health Repository)

    Tangney, Mark

    2012-01-31

    Cancer remains a leading cause of morbidity and mortality. Despite advances in understanding, detection, and treatment, it accounts for almost one-fourth of all deaths per year in Western countries. Prostate cancer is currently the most commonly diagnosed noncutaneous cancer in men in Europe and the United States, accounting for 15% of all cancers in men. As life expectancy of individuals increases, it is expected that there will also be an increase in the incidence and mortality of prostate cancer. Prostate cancer may be inoperable at initial presentation, unresponsive to chemotherapy and radiotherapy, or recur following appropriate treatment. At the time of presentation, patients may already have metastases in their tissues. Preventing tumor recurrence requires systemic therapy; however, current modalities are limited by toxicity or lack of efficacy. For patients with such metastatic cancers, the development of alternative therapies is essential. Gene therapy is a realistic prospect for the treatment of prostate and other cancers, and involves the delivery of genetic information to the patient to facilitate the production of therapeutic proteins. Therapeutics can act directly (eg, by inducing tumor cells to produce cytotoxic agents) or indirectly by upregulating the immune system to efficiently target tumor cells or by destroying the tumor\\'s vasculature. However, technological difficulties must be addressed before an efficient and safe gene medicine is achieved (primarily by developing a means of delivering genes to the target cells or tissue safely and efficiently). A wealth of research has been carried out over the past 20 years, involving various strategies for the treatment of prostate cancer at preclinical and clinical trial levels. The therapeutic efficacy observed with many of these approaches in patients indicates that these treatment modalities will serve as an important component of urological malignancy treatment in the clinic, either in isolation or

  4. Evolutionary signatures amongst disease genes permit novel methods for gene prioritization and construction of informative gene-based networks.

    Directory of Open Access Journals (Sweden)

    Nolan Priedigkeit

    2015-02-01

    Full Text Available Genes involved in the same function tend to have similar evolutionary histories, in that their rates of evolution covary over time. This coevolutionary signature, termed Evolutionary Rate Covariation (ERC, is calculated using only gene sequences from a set of closely related species and has demonstrated potential as a computational tool for inferring functional relationships between genes. To further define applications of ERC, we first established that roughly 55% of genetic diseases posses an ERC signature between their contributing genes. At a false discovery rate of 5% we report 40 such diseases including cancers, developmental disorders and mitochondrial diseases. Given these coevolutionary signatures between disease genes, we then assessed ERC's ability to prioritize known disease genes out of a list of unrelated candidates. We found that in the presence of an ERC signature, the true disease gene is effectively prioritized to the top 6% of candidates on average. We then apply this strategy to a melanoma-associated region on chromosome 1 and identify MCL1 as a potential causative gene. Furthermore, to gain global insight into disease mechanisms, we used ERC to predict molecular connections between 310 nominally distinct diseases. The resulting "disease map" network associates several diseases with related pathogenic mechanisms and unveils many novel relationships between clinically distinct diseases, such as between Hirschsprung's disease and melanoma. Taken together, these results demonstrate the utility of molecular evolution as a gene discovery platform and show that evolutionary signatures can be used to build informative gene-based networks.

  5. Lineage-specific expansion of IFIT gene family: an insight into coevolution with IFN gene family.

    Directory of Open Access Journals (Sweden)

    Ying Liu

    Full Text Available In mammals, IFIT (Interferon [IFN]-induced proteins with Tetratricopeptide Repeat [TPR] motifs family genes are involved in many cellular and viral processes, which are tightly related to mammalian IFN response. However, little is known about non-mammalian IFIT genes. In the present study, IFIT genes are identified in the genome databases from the jawed vertebrates including the cartilaginous elephant shark but not from non-vertebrates such as lancelet, sea squirt and acorn worm, suggesting that IFIT gene family originates from a vertebrate ancestor about 450 million years ago. IFIT family genes show conserved gene structure and gene arrangements. Phylogenetic analyses reveal that this gene family has expanded through lineage-specific and species-specific gene duplication. Interestingly, IFN gene family seem to share a common ancestor and a similar evolutionary mechanism; the function link of IFIT genes to IFN response is present early since the origin of both gene families, as evidenced by the finding that zebrafish IFIT genes are upregulated by fish IFNs, poly(I:C and two transcription factors IRF3/IRF7, likely via the IFN-stimulated response elements (ISRE within the promoters of vertebrate IFIT family genes. These coevolution features creates functional association of both family genes to fulfill a common biological process, which is likely selected by viral infection during evolution of vertebrates. Our results are helpful for understanding of evolution of vertebrate IFN system.

  6. Multiobjective differential evolution-based multifactor dimensionality reduction for detecting gene-gene interactions.

    Science.gov (United States)

    Yang, Cheng-Hong; Chuang, Li-Yeh; Lin, Yu-Da

    2017-10-09

    Epistasis within disease-related genes (gene-gene interactions) was determined through contingency table measures based on multifactor dimensionality reduction (MDR) using single-nucleotide polymorphisms (SNPs). Most MDR-based methods use the single contingency table measure to detect gene-gene interactions; however, some gene-gene interactions may require identification through multiple contingency table measures. In this study, a multiobjective differential evolution method (called MODEMDR) was proposed to merge the various contingency table measures based on MDR to detect significant gene-gene interactions. Two contingency table measures, namely the correct classification rate and normalized mutual information, were selected to design the fitness functions in MODEMDR. The characteristics of multiobjective optimization enable MODEMDR to use multiple measures to efficiently and synchronously detect significant gene-gene interactions within a reasonable time frame. Epistatic models with and without marginal effects under various parameter settings (heritability and minor allele frequencies) were used to assess existing methods by comparing the detection success rates of gene-gene interactions. The results of the simulation datasets show that MODEMDR is superior to existing methods. Moreover, a large dataset obtained from the Wellcome Trust Case Control Consortium was used to assess MODEMDR. MODEMDR exhibited efficiency in identifying significant gene-gene interactions in genome-wide association studies.

  7. With Reference to Reference Genes: A Systematic Review of Endogenous Controls in Gene Expression Studies.

    Science.gov (United States)

    Chapman, Joanne R; Waldenström, Jonas

    2015-01-01

    The choice of reference genes that are stably expressed amongst treatment groups is a crucial step in real-time quantitative PCR gene expression studies. Recent guidelines have specified that a minimum of two validated reference genes should be used for normalisation. However, a quantitative review of the literature showed that the average number of reference genes used across all studies was 1.2. Thus, the vast majority of studies continue to use a single gene, with β-actin (ACTB) and/or glyceraldehyde 3-phosphate dehydrogenase (GAPDH) being commonly selected in studies of vertebrate gene expression. Few studies (15%) tested a panel of potential reference genes for stability of expression before using them to normalise data. Amongst studies specifically testing reference gene stability, few found ACTB or GAPDH to be optimal, whereby these genes were significantly less likely to be chosen when larger panels of potential reference genes were screened. Fewer reference genes were tested for stability in non-model organisms, presumably owing to a dearth of available primers in less well characterised species. Furthermore, the experimental conditions under which real-time quantitative PCR analyses were conducted had a large influence on the choice of reference genes, whereby different studies of rat brain tissue showed different reference genes to be the most stable. These results highlight the importance of validating the choice of normalising reference genes before conducting gene expression studies.

  8. QB1 - Stochastic Gene Regulation

    Energy Technology Data Exchange (ETDEWEB)

    Munsky, Brian [Los Alamos National Laboratory

    2012-07-23

    Summaries of this presentation are: (1) Stochastic fluctuations or 'noise' is present in the cell - Random motion and competition between reactants, Low copy, quantization of reactants, Upstream processes; (2) Fluctuations may be very important - Cell-to-cell variability, Cell fate decisions (switches), Signal amplification or damping, stochastic resonances; and (3) Some tools are available to mode these - Kinetic Monte Carlo simulations (SSA and variants), Moment approximation methods, Finite State Projection. We will see how modeling these reactions can tell us more about the underlying processes of gene regulation.

  9. Mathematical Models of Gene Regulation

    Science.gov (United States)

    Mackey, Michael C.

    2004-03-01

    This talk will focus on examples of mathematical models for the regulation of repressible operons (e.g. the tryptophan operon), inducible operons (e.g. the lactose operon), and the lysis/lysogeny switch in phage λ. These ``simple" gene regulatory elements can display characteristics experimentally of rapid response to perturbations and bistability, and biologically accurate mathematical models capture these aspects of the dynamics. The models, if realistic, are always nonlinear and contain significant time delays due to transcriptional and translational delays that pose substantial problems for the analysis of the possible ranges of dynamics.

  10. Plant domestication and gene banks

    International Nuclear Information System (INIS)

    Perrino, P.

    1989-01-01

    At the time of the dawn of agriculture, plant domestication was very slow. As agriculture progressed, however, domestication began to evolve faster and reached its highest point with the advent of plant breeders who played a very important role in solving the world food problem. One of the fastest moving strategies was a better exploitation of genetic diversity, both natural and induced. However, intensive plant breeding activity caused a heavy fall in genetic variability. Gene banks then provided a further tool for modern agriculture, specifically to preserve genetic resources and to help breeders to further domesticate important crops and to introduce and domesticate new species. (author). 3 refs

  11. Impact of gene family evolutionary histories on phylogenetic species tree inference by gene tree parsimony.

    Science.gov (United States)

    Shi, Tao

    2016-03-01

    Complicated history of gene duplication and loss brings challenge to molecular phylogenetic inference, especially in deep phylogenies. However, phylogenomic approaches, such as gene tree parsimony (GTP), show advantage over some other approaches in its ability to use gene families with duplications. GTP searches the 'optimal' species tree by minimizing the total cost of biological events such as duplications, but accuracy of GTP and phylogenetic signal in the context of different gene families with distinct histories of duplication and loss are unclear. To evaluate how different evolutionary properties of different gene families can impact on species tree inference, 3900 gene families from seven angiosperms encompassing a wide range of gene content, lineage-specific expansions and contractions were analyzed. It was found that the gene content and total duplication number in a gene family strongly influence species tree inference accuracy, with the highest accuracy achieved at either very low or very high gene content (or duplication number) and lowest accuracy centered in intermediate gene content (or duplication number), as the relationship can fit a binomial regression. Besides, for gene families of similar level of average gene content, those with relatively higher lineage-specific expansion or duplication rates tend to show lower accuracy. Additional correlation tests support that high accuracy for those gene families with large gene content may rely on abundant ancestral copies to provide many subtrees to resolve conflicts, whereas high accuracy for single or low copy gene families are just subject to sequence substitution per se. Very low accuracy reached by gene families of intermediate gene content or duplication number can be due to insufficient subtrees to resolve the conflicts from loss of alternative copies. As these evolutionary properties can significantly influence species tree accuracy, I discussed the potential weighting of the duplication cost by

  12. Identification of Human HK Genes and Gene Expression Regulation Study in Cancer from Transcriptomics Data Analysis

    Science.gov (United States)

    Zhang, Zhang; Liu, Jingxing; Wu, Jiayan; Yu, Jun

    2013-01-01

    The regulation of gene expression is essential for eukaryotes, as it drives the processes of cellular differentiation and morphogenesis, leading to the creation of different cell types in multicellular organisms. RNA-Sequencing (RNA-Seq) provides researchers with a powerful toolbox for characterization and quantification of transcriptome. Many different human tissue/cell transcriptome datasets coming from RNA-Seq technology are available on public data resource. The fundamental issue here is how to develop an effective analysis method to estimate expression pattern similarities between different tumor tissues and their corresponding normal tissues. We define the gene expression pattern from three directions: 1) expression breadth, which reflects gene expression on/off status, and mainly concerns ubiquitously expressed genes; 2) low/high or constant/variable expression genes, based on gene expression level and variation; and 3) the regulation of gene expression at the gene structure level. The cluster analysis indicates that gene expression pattern is higher related to physiological condition rather than tissue spatial distance. Two sets of human housekeeping (HK) genes are defined according to cell/tissue types, respectively. To characterize the gene expression pattern in gene expression level and variation, we firstly apply improved K-means algorithm and a gene expression variance model. We find that cancer-associated HK genes (a HK gene is specific in cancer group, while not in normal group) are expressed higher and more variable in cancer condition than in normal condition. Cancer-associated HK genes prefer to AT-rich genes, and they are enriched in cell cycle regulation related functions and constitute some cancer signatures. The expression of large genes is also avoided in cancer group. These studies will help us understand which cell type-specific patterns of gene expression differ among different cell types, and particularly for cancer. PMID:23382867

  13. Reporter Gene Assays in Ecotoxicology.

    Science.gov (United States)

    Elad, Tal; Belkin, Shimshon

    The need for simple and rapid means for evaluating the potential toxic effects of environmental samples has prompted the development of reporter gene assays, based on tester cells (bioreporters) genetically engineered to report on sample toxicity by producing a readily quantifiable signal. Bacteria are especially suitable to serve as bioreporters owing to their fast responses, low cost, convenient preservation, ease of handling, and amenability to genetic manipulations. Various bacterial bioreporters have been introduced for general toxicity and genotoxicity assessment, and the monitoring of endocrine disrupting and dioxin-like compounds has been mostly covered by similarly engineered eukaryotic cells. Some reporter gene assays have been validated, standardized, and accredited, and many others are under constant development. Efforts are aimed at broadening detection spectra, lowering detection thresholds, and combining toxicity identification capabilities with characterization of the toxic effects. Taking advantage of bacterial robustness, attempts are also being made to incorporate bacterial bioreporters into field instrumentation for online continuous monitoring or on-site spot checks. However, key hurdles concerning test validation, cell preservation, and regulatory issues related to the use of genetically modified organisms still remain to be overcome.

  14. Gene Ontology annotations and resources.

    Science.gov (United States)

    Blake, J A; Dolan, M; Drabkin, H; Hill, D P; Li, Ni; Sitnikov, D; Bridges, S; Burgess, S; Buza, T; McCarthy, F; Peddinti, D; Pillai, L; Carbon, S; Dietze, H; Ireland, A; Lewis, S E; Mungall, C J; Gaudet, P; Chrisholm, R L; Fey, P; Kibbe, W A; Basu, S; Siegele, D A; McIntosh, B K; Renfro, D P; Zweifel, A E; Hu, J C; Brown, N H; Tweedie, S; Alam-Faruque, Y; Apweiler, R; Auchinchloss, A; Axelsen, K; Bely, B; Blatter, M -C; Bonilla, C; Bouguerleret, L; Boutet, E; Breuza, L; Bridge, A; Chan, W M; Chavali, G; Coudert, E; Dimmer, E; Estreicher, A; Famiglietti, L; Feuermann, M; Gos, A; Gruaz-Gumowski, N; Hieta, R; Hinz, C; Hulo, C; Huntley, R; James, J; Jungo, F; Keller, G; Laiho, K; Legge, D; Lemercier, P; Lieberherr, D; Magrane, M; Martin, M J; Masson, P; Mutowo-Muellenet, P; O'Donovan, C; Pedruzzi, I; Pichler, K; Poggioli, D; Porras Millán, P; Poux, S; Rivoire, C; Roechert, B; Sawford, T; Schneider, M; Stutz, A; Sundaram, S; Tognolli, M; Xenarios, I; Foulgar, R; Lomax, J; Roncaglia, P; Khodiyar, V K; Lovering, R C; Talmud, P J; Chibucos, M; Giglio, M Gwinn; Chang, H -Y; Hunter, S; McAnulla, C; Mitchell, A; Sangrador, A; Stephan, R; Harris, M A; Oliver, S G; Rutherford, K; Wood, V; Bahler, J; Lock, A; Kersey, P J; McDowall, D M; Staines, D M; Dwinell, M; Shimoyama, M; Laulederkind, S; Hayman, T; Wang, S -J; Petri, V; Lowry, T; D'Eustachio, P; Matthews, L; Balakrishnan, R; Binkley, G; Cherry, J M; Costanzo, M C; Dwight, S S; Engel, S R; Fisk, D G; Hitz, B C; Hong, E L; Karra, K; Miyasato, S R; Nash, R S; Park, J; Skrzypek, M S; Weng, S; Wong, E D; Berardini, T Z; Huala, E; Mi, H; Thomas, P D; Chan, J; Kishore, R; Sternberg, P; Van Auken, K; Howe, D; Westerfield, M

    2013-01-01

    The Gene Ontology (GO) Consortium (GOC, http://www.geneontology.org) is a community-based bioinformatics resource that classifies gene product function through the use of structured, controlled vocabularies. Over the past year, the GOC has implemented several processes to increase the quantity, quality and specificity of GO annotations. First, the number of manual, literature-based annotations has grown at an increasing rate. Second, as a result of a new 'phylogenetic annotation' process, manually reviewed, homology-based annotations are becoming available for a broad range of species. Third, the quality of GO annotations has been improved through a streamlined process for, and automated quality checks of, GO annotations deposited by different annotation groups. Fourth, the consistency and correctness of the ontology itself has increased by using automated reasoning tools. Finally, the GO has been expanded not only to cover new areas of biology through focused interaction with experts, but also to capture greater specificity in all areas of the ontology using tools for adding new combinatorial terms. The GOC works closely with other ontology developers to support integrated use of terminologies. The GOC supports its user community through the use of e-mail lists, social media and web-based resources.

  15. The iojap gene in maize

    Energy Technology Data Exchange (ETDEWEB)

    Martienssen, Robert

    2001-12-01

    The classical maize mutant iojap (Iodent japonica) has variegated green and white leaves. Green sectors have cells with normal chloroplasts whereas white sectors have cells where plastids fail to differentiate. These mutant plastids, when transmitted through the female gametophyte, do not recover in the presence of wild type Iojap. We cloned the Ij locus, and we have investigated the mechanism of epigenetic inheritance and phenotypic expression. More recently, a modifier of this type of variegation, ''Inhibitor of striate'', has also been cloned. Both the iojap and inhibitor of striate proteins have homologs in bacteria and are members of ancient conserved families found in multiple species. These tools can be used to address fundamental questions of inheritance and variegation associated with this classical conundrum of maize genetics. Since the work of Rhoades there has been considerable speculation concerning the nature of the Iojap gene product, the origin of leaf variegation and the mechanism behind the material inheritance of defective plastids. This has made Iojap a textbook paradigm for cytoplasmic inheritance and nuclear-organellar interaction for almost 50 years. Cloning of the Iojap gene in maize, and homologs in other plants and bacteria, provides a new means to address the origin of heteroplastidity, variegation and cytoplasmic inheritance in higher plants.

  16. Obesity genes and insulin resistance.

    Science.gov (United States)

    Belkina, Anna C; Denis, Gerald V

    2010-10-01

    The exploding prevalence of insulin resistance and Type 2 diabetes (T2D) linked to obesity has become an alarming public health concern. Worldwide, approximately 171 million people suffer from obesity-induced diabetes and public health authorities expect this situation to deteriorate rapidly. An interesting clinical population of 'metabolically healthy but obese' (MHO) cases is relatively protected from T2D and its associated cardiovascular risk. The molecular basis for this protection is not well understood but is likely to involve reduced inflammatory responses. The inflammatory cells and pathways that respond to overnutrition are the primary subject matter for this review. The chance discovery of a genetic mutation in the Brd2 gene, which is located in the class II major histocompatibility complex and makes mice enormously fat but protects them from diabetes, offers revolutionary new insights into the cellular mechanisms that link obesity to insulin resistance and T2D. These Brd2-hypomorphic mice have reduced inflammation in fat that is normally associated with insulin resistance, and resemble MHO patients, suggesting novel therapeutic pathways for obese patients at risk for T2D. Deeper understanding of the functional links between genes that control inflammatory responses to diet-induced obesity is crucial to the development of therapies for obese, insulin-resistant patients.

  17. Targeting Herpetic Keratitis by Gene Therapy

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    Hossein Mostafa Elbadawy

    2012-01-01

    Full Text Available Ocular gene therapy is rapidly becoming a reality. By November 2012, approximately 28 clinical trials were approved to assess novel gene therapy agents. Viral infections such as herpetic keratitis caused by herpes simplex virus 1 (HSV-1 can cause serious complications that may lead to blindness. Recurrence of the disease is likely and cornea transplantation, therefore, might not be the ideal therapeutic solution. This paper will focus on the current situation of ocular gene therapy research against herpetic keratitis, including the use of viral and nonviral vectors, routes of delivery of therapeutic genes, new techniques, and key research strategies. Whereas the correction of inherited diseases was the initial goal of the field of gene therapy, here we discuss transgene expression, gene replacement, silencing, or clipping. Gene therapy of herpetic keratitis previously reported in the literature is screened emphasizing candidate gene therapy targets. Commonly adopted strategies are discussed to assess the relative advantages of the protective therapy using antiviral drugs and the common gene therapy against long-term HSV-1 ocular infections signs, inflammation and neovascularization. Successful gene therapy can provide innovative physiological and pharmaceutical solutions against herpetic keratitis.

  18. Fusion genes in leukemia: an emerging network.

    Science.gov (United States)

    Bohlander, S K

    2000-01-01

    The molecular analysis of recurring chromosome rearrangements, especially of translocations and inversions, has provided us with valuable insight into the pathogenesis of hematological malignancies. Many translocations result in the fusion of genes located at the translocation breakpoints. In recent years we have witnessed a rapid rise in the number of chromosome translocations in leukemias being characterized at the molecular level. However, the number of genes being newly identified as translocation fusion genes has not risen at the same pace. This is due to the fact that several genes are involved in more than one translocation forming fusion genes with a number of other partner genes. Not only does one find star-shaped topologies, with one gene forming fusions with several others (e.g. ETV6/PDGFRB, ETV6/JAK2, ETV6/ABL etc.), but also networks connecting several genes with more than one fusion partner (e.g. ETV6/RUNX1 (AML1), RUNX1/CBFA2T1 (ETO), ETV6/EVI1, RUNX1/EVI1, ETV6/ABL, BCR/ABL). The emergence of such networks with the "recycling" of genes in new fusion combinations suggests that there is a rather limited number of genes which can be altered to cause leukemia. Copyright 2001 S. Karger AG, Basel

  19. Evolution of the Vertebrate Resistin Gene Family.

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    Qingda Hu

    Full Text Available Resistin (encoded by Retn was previously identified in rodents as a hormone associated with diabetes; however human resistin is instead linked to inflammation. Resistin is a member of a small gene family that includes the resistin-like peptides (encoded by Retnl genes in mammals. Genomic searches of available genome sequences of diverse vertebrates and phylogenetic analyses were conducted to determine the size and origin of the resistin-like gene family. Genes encoding peptides similar to resistin were found in Mammalia, Sauria, Amphibia, and Actinistia (coelacanth, a lobe-finned fish, but not in Aves or fish from Actinopterygii, Chondrichthyes, or Agnatha. Retnl originated by duplication and transposition from Retn on the early mammalian lineage after divergence of the platypus, but before the placental and marsupial mammal divergence. The resistin-like gene family illustrates an instance where the locus of origin of duplicated genes can be identified, with Retn continuing to reside at this location. Mammalian species typically have a single copy Retn gene, but are much more variable in their numbers of Retnl genes, ranging from 0 to 9. Since Retn is located at the locus of origin, thus likely retained the ancestral expression pattern, largely maintained its copy number, and did not display accelerated evolution, we suggest that it is more likely to have maintained an ancestral function, while Retnl, which transposed to a new location, displays accelerated evolution, and shows greater variability in gene number, including gene loss, likely evolved new, but potentially lineage-specific, functions.

  20. Arabidopsis gene expression patterns during spaceflight

    Science.gov (United States)

    Paul, A.-L.; Ferl, R. J.

    The exposure of Arabidopsis thaliana (Arabidopsis) plants to spaceflight environments resulted in the differential expression of hundreds of genes. A 5 day mission on orbiter Columbia in 1999 (STS-93) carried transgenic Arabidopsis plants engineered with a transgene composed of the alcohol dehydrogenase (Adh) gene promoter linked to the β -Glucuronidase (GUS) reporter gene. The plants were used to evaluate the effects of spaceflight on two fronts. First, expression patterns visualized with the Adh/GUS transgene were used to address specifically the possibility that spaceflight induces a hypoxic stress response, and to assess whether any spaceflight response was similar to control terrestrial hypoxia-induced gene expression patterns. (Paul et al., Plant Physiol. 2001, 126:613). Second, genome-wide patterns of native gene expression were evaluated utilizing the Affymetrix ATH1 GeneChip? array of 8,000 Arabidopsis genes. As a control for the veracity of the array analyses, a selection of genes identified with the arrays was further characterized with quantitative Real-Time RT PCR (ABI - TaqmanTM). Comparison of the patterns of expression for arrays of hybridized with RNA isolated from plants exposed to spaceflight compared to the control arrays revealed hundreds of genes that were differentially expressed in response to spaceflight, yet most genes that are hallmarks of hypoxic stress were unaffected. These results will be discussed in light of current models for plant responses to the spaceflight environment, and with regard to potential future flight opportunities.

  1. Switching on the lights for gene therapy.

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    Alexandra Winkeler

    Full Text Available Strategies for non-invasive and quantitative imaging of gene expression in vivo have been developed over the past decade. Non-invasive assessment of the dynamics of gene regulation is of interest for the detection of endogenous disease-specific biological alterations (e.g., signal transduction and for monitoring the induction and regulation of therapeutic genes (e.g., gene therapy. To demonstrate that non-invasive imaging of regulated expression of any type of gene after in vivo transduction by versatile vectors is feasible, we generated regulatable herpes simplex virus type 1 (HSV-1 amplicon vectors carrying hormone (mifepristone or antibiotic (tetracycline regulated promoters driving the proportional co-expression of two marker genes. Regulated gene expression was monitored by fluorescence microscopy in culture and by positron emission tomography (PET or bioluminescence (BLI in vivo. The induction levels evaluated in glioma models varied depending on the dose of inductor. With fluorescence microscopy and BLI being the tools for assessing gene expression in culture and animal models, and with PET being the technology for possible application in humans, the generated vectors may serve to non-invasively monitor the dynamics of any gene of interest which is proportionally co-expressed with the respective imaging marker gene in research applications aiming towards translation into clinical application.

  2. The history of the Mendelian gene.

    Science.gov (United States)

    Frías L, Daniel

    2007-01-01

    The concept of heredity arose when the ancient philosophers and scientists felt the need to explain the variation and organic evolution phenomena. The ideas about inheritance developed before Mendel were significant in the construction of the Mendelian concept of gene. From Mendelian hereditary principles to molecular genetics there have been many different concepts and also many definitions of gene. In the first corpuscular concept of gene, mutation was quite crucial to explain the different alternative genotype and phenotype expression in the progeny. From the rediscovery of Mendelian Principles to 1961, Morgan's idea that a gene is not divisible by recombination prevailed. Nevertheless it was later demonstrated that there are different units of recombination and mutation within the gene, and in a determinate gene different "functional units" can exist. In 1977, surprisingly, Sharp and Roberts found out that genes are fragmented into "exons" and "introns". At present time, with the discovery of iRNA, non coding RNA, importance of introns, transposable elements, pseudogenes, endogenous viral DNA, repeated DNA, superposed genes, non-transcriptional genes and epigenesis, ancient questions return: what is a gene? where is the program? what is the true role of mutations in the organic evolution?

  3. Human DNA repair and recombination genes

    International Nuclear Information System (INIS)

    Thompson, L.H.; Weber, C.A.; Jones, N.J.

    1988-09-01

    Several genes involved in mammalian DNA repair pathways were identified by complementation analysis and chromosomal mapping based on hybrid cells. Eight complementation groups of rodent mutants defective in the repair of uv radiation damage are now identified. At least seven of these genes are probably essential for repair and at least six of them control the incision step. The many genes required for repair of DNA cross-linking damage show overlap with those involved in the repair of uv damage, but some of these genes appear to be unique for cross-link repair. Two genes residing on human chromosome 19 were cloned from genomic transformants using a cosmid vector, and near full-length cDNA clones of each gene were isolated and sequenced. Gene ERCC2 efficiently corrects the defect in CHO UV5, a nucleotide excision repair mutant. Gene XRCC1 normalizes repair of strand breaks and the excessive sister chromatid exchange in CHO mutant EM9. ERCC2 shows a remarkable /approximately/52% overall homology at both the amino acid and nucleotide levels with the yeast RAD3 gene. Evidence based on mutation induction frequencies suggests that ERCC2, like RAD3, might also be an essential gene for viability. 100 refs., 4 tabs

  4. Die Rolle der microRNAs und Dicer in Entzündungszellen von Atemwegserkrankungen

    OpenAIRE

    Thiele, Stefanie

    2010-01-01

    Atemwegserkrankungen stellen ein weltweites Gesundheitsproblem dar und sind durch die Infiltration der Atemwege durch Immunzellen und die daraus resultierende Entzündung charakterisiert. Antientzündliche Therapieansätze sind daher am wichtigsten und effektivsten. Speziell für die Behandlung von Atemwegserkrankungen am Patienten ist eine effiziente topische Verabreichung von Therapeutika über die Atemwege wünschenswert. Small interfering RNAs (siRNAs) können Gene posttranskriptionell reguliere...

  5. Selection of Phototransduction Genes in Homo sapiens.

    Science.gov (United States)

    Christopher, Mark; Scheetz, Todd E; Mullins, Robert F; Abràmoff, Michael D

    2013-08-13

    We investigated the evidence of recent positive selection in the human phototransduction system at single nucleotide polymorphism (SNP) and gene level. SNP genotyping data from the International HapMap Project for European, Eastern Asian, and African populations was used to discover differences in haplotype length and allele frequency between these populations. Numeric selection metrics were computed for each SNP and aggregated into gene-level metrics to measure evidence of recent positive selection. The level of recent positive selection in phototransduction genes was evaluated and compared to a set of genes shown previously to be under recent selection, and a set of highly conserved genes as positive and negative controls, respectively. Six of 20 phototransduction genes evaluated had gene-level selection metrics above the 90th percentile: RGS9, GNB1, RHO, PDE6G, GNAT1, and SLC24A1. The selection signal across these genes was found to be of similar magnitude to the positive control genes and much greater than the negative control genes. There is evidence for selective pressure in the genes involved in retinal phototransduction, and traces of this selective pressure can be demonstrated using SNP-level and gene-level metrics of allelic variation. We hypothesize that the selective pressure on these genes was related to their role in low light vision and retinal adaptation to ambient light changes. Uncovering the underlying genetics of evolutionary adaptations in phototransduction not only allows greater understanding of vision and visual diseases, but also the development of patient-specific diagnostic and intervention strategies.

  6. PoplarGene: poplar gene network and resource for mining functional information for genes from woody plants

    OpenAIRE

    Qi Liu; Changjun Ding; Yanguang Chu; Jiafei Chen; Weixi Zhang; Bingyu Zhang; Qinjun Huang; Xiaohua Su

    2016-01-01

    Poplar is not only an important resource for the production of paper, timber and other wood-based products, but it has also emerged as an ideal model system for studying woody plants. To better understand the biological processes underlying various traits in poplar, e.g., wood development, a comprehensive functional gene interaction network is highly needed. Here, we constructed a genome-wide functional gene network for poplar (covering ~70% of the 41,335 poplar genes) and created the network...

  7. Evaluating the consistency of gene sets used in the analysis of bacterial gene expression data

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    Tintle Nathan L

    2012-08-01

    Full Text Available Abstract Background Statistical analyses of whole genome expression data require functional information about genes in order to yield meaningful biological conclusions. The Gene Ontology (GO and Kyoto Encyclopedia of Genes and Genomes (KEGG are common sources of functionally grouped gene sets. For bacteria, the SEED and MicrobesOnline provide alternative, complementary sources of gene sets. To date, no comprehensive evaluation of the data obtained from these resources has been performed. Results We define a series of gene set consistency metrics directly related to the most common classes of statistical analyses for gene expression data, and then perform a comprehensive analysis of 3581 Affymetrix® gene expression arrays across 17 diverse bacteria. We find that gene sets obtained from GO and KEGG demonstrate lower consistency than those obtained from the SEED and MicrobesOnline, regardless of gene set size. Conclusions Despite the widespread use of GO and KEGG gene sets in bacterial gene expression data analysis, the SEED and MicrobesOnline provide more consistent sets for a wide variety of statistical analyses. Increased use of the SEED and MicrobesOnline gene sets in the analysis of bacterial gene expression data may improve statistical power and utility of expression data.

  8. Identification of nitrogen-fixing genes and gene clusters from metagenomic library of acid mine drainage.

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    Zhimin Dai

    Full Text Available Biological nitrogen fixation is an essential function of acid mine drainage (AMD microbial communities. However, most acidophiles in AMD environments are uncultured microorganisms and little is known about the diversity of nitrogen-fixing genes and structure of nif gene cluster in AMD microbial communities. In this study, we used metagenomic sequencing to isolate nif genes in the AMD microbial community from Dexing Copper Mine, China. Meanwhile, a metagenome microarray containing 7,776 large-insertion fosmids was constructed to screen novel nif gene clusters. Metagenomic analyses revealed that 742 sequences were identified as nif genes including structural subunit genes nifH, nifD, nifK and various additional genes. The AMD community is massively dominated by the genus Acidithiobacillus. However, the phylogenetic diversity of nitrogen-fixing microorganisms is much higher than previously thought in the AMD community. Furthermore, a 32.5-kb genomic sequence harboring nif, fix and associated genes was screened by metagenome microarray. Comparative genome analysis indicated that most nif genes in this cluster are most similar to those of Herbaspirillum seropedicae, but the organization of the nif gene cluster had significant differences from H. seropedicae. Sequence analysis and reverse transcription PCR also suggested that distinct transcription units of nif genes exist in this gene cluster. nifQ gene falls into the same transcription unit with fixABCX genes, which have not been reported in other diazotrophs before. All of these results indicated that more novel diazotrophs survive in the AMD community.

  9. Functional analysis of the molecular interactions of TATA box-containing genes and essential genes.

    Science.gov (United States)

    Bae, Sang-Hun; Han, Hyun Wook; Moon, Jisook

    2015-01-01

    Genes can be divided into TATA-containing genes and TATA-less genes according to the presence of TATA box elements at promoter regions. TATA-containing genes tend to be stress-responsive, whereas many TATA-less genes are known to be related to cell growth or "housekeeping" functions. In a previous study, we demonstrated that there are striking differences among four gene sets defined by the presence of TATA box (TATA-containing) and essentiality (TATA-less) with respect to number of associated transcription factors, amino acid usage, and functional annotation. Extending this research in yeast, we identified KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways that are statistically enriched in TATA-containing or TATA-less genes and evaluated the possibility that the enriched pathways are related to stress or growth as reflected by the individual functions of the genes involved. According to their enrichment for either of these two gene sets, we sorted KEGG pathways into TATA-containing-gene-enriched pathways (TEPs) and essential-gene-enriched pathways (EEPs). As expected, genes in TEPs and EEPs exhibited opposite results in terms of functional category, transcriptional regulation, codon adaptation index, and network properties, suggesting the possibility that the bipolar patterns in these pathways also contribute to the regulation of the stress response and to cell survival. Our findings provide the novel insight that significant enrichment of TATA-binding or TATA-less genes defines pathways as stress-responsive or growth-related.

  10. Using GeneReg to construct time delay gene regulatory networks

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    Qian Ziliang

    2010-05-01

    Full Text Available Abstract Background Understanding gene expression and regulation is essential for understanding biological mechanisms. Because gene expression profiling has been widely used in basic biological research, especially in transcription regulation studies, we have developed GeneReg, an easy-to-use R package, to construct gene regulatory networks from time course gene expression profiling data; More importantly, this package can provide information about time delays between expression change in a regulator and that of its target genes. Findings The R package GeneReg is based on time delay linear regression, which can generate a model of the expression levels of regulators at a given time point against the expression levels of their target genes at a later time point. There are two parameters in the model, time delay and regulation coefficient. Time delay is the time lag during which expression change of the regulator is transmitted to change in target gene expression. Regulation coefficient expresses the regulation effect: a positive regulation coefficient indicates activation and negative indicates repression. GeneReg was implemented on a real Saccharomyces cerevisiae cell cycle dataset; more than thirty percent of the modeled regulations, based entirely on gene expression files, were found to be consistent with previous discoveries from known databases. Conclusions GeneReg is an easy-to-use, simple, fast R package for gene regulatory network construction from short time course gene expression data. It may be applied to study time-related biological processes such as cell cycle, cell differentiation, or causal inference.

  11. Horizontal acquisition of multiple mitochondrial genes from a parasitic plant followed by gene conversion with host mitochondrial genes

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    Hao Weilong

    2010-12-01

    Full Text Available Abstract Background Horizontal gene transfer (HGT is relatively common in plant mitochondrial genomes but the mechanisms, extent and consequences of transfer remain largely unknown. Previous results indicate that parasitic plants are often involved as either transfer donors or recipients, suggesting that direct contact between parasite and host facilitates genetic transfer among plants. Results In order to uncover the mechanistic details of plant-to-plant HGT, the extent and evolutionary fate of transfer was investigated between two groups: the parasitic genus Cuscuta and a small clade of Plantago species. A broad polymerase chain reaction (PCR survey of mitochondrial genes revealed that at least three genes (atp1, atp6 and matR were recently transferred from Cuscuta to Plantago. Quantitative PCR assays show that these three genes have a mitochondrial location in the one species line of Plantago examined. Patterns of sequence evolution suggest that these foreign genes degraded into pseudogenes shortly after transfer and reverse transcription (RT-PCR analyses demonstrate that none are detectably transcribed. Three cases of gene conversion were detected between native and foreign copies of the atp1 gene. The identical phylogenetic distribution of the three foreign genes within Plantago and the retention of cytidines at ancestral positions of RNA editing indicate that these genes were probably acquired via a single, DNA-mediated transfer event. However, samplings of multiple individuals from two of the three species in the recipient Plantago clade revealed complex and perplexing phylogenetic discrepancies and patterns of sequence divergence for all three of the foreign genes. Conclusions This study reports the best evidence to date that multiple mitochondrial genes can be transferred via a single HGT event and that transfer occurred via a strictly DNA-level intermediate. The discovery of gene conversion between co-resident foreign and native

  12. Prediction of the gene expression in normal lung tissue by the gene expression in blood.

    Science.gov (United States)

    Halloran, Justin W; Zhu, Dakai; Qian, David C; Byun, Jinyoung; Gorlova, Olga Y; Amos, Christopher I; Gorlov, Ivan P

    2015-11-17

    Comparative analysis of gene expression in human tissues is important for understanding the molecular mechanisms underlying tissue-specific control of gene expression. It can also open an avenue for using gene expression in blood (which is the most easily accessible human tissue) to predict gene expression in other (less accessible) tissues, which would facilitate the development of novel gene expression based models for assessing disease risk and progression. Until recently, direct comparative analysis across different tissues was not possible due to the scarcity of paired tissue samples from the same individuals. In this study we used paired whole blood/lung gene expression data from the Genotype-Tissue Expression (GTEx) project. We built a generalized linear regression model for each gene using gene expression in lung as the outcome and gene expression in blood, age and gender as predictors. For ~18 % of the genes, gene expression in blood was a significant predictor of gene expression in lung. We found that the number of single nucleotide polymorphisms (SNPs) influencing expression of a given gene in either blood or lung, also known as the number of quantitative trait loci (eQTLs), was positively associated with efficacy of blood-based prediction of that gene's expression in lung. This association was strongest for shared eQTLs: those influencing gene expression in both blood and lung. In conclusion, for a considerable number of human genes, their expression levels in lung can be predicted using observable gene expression in blood. An abundance of shared eQTLs may explain the strong blood/lung correlations in the gene expression.

  13. Using RNA-seq data to select reference genes for normalizing gene expression in apple roots.

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    Zhe Zhou

    Full Text Available Gene expression in apple roots in response to various stress conditions is a less-explored research subject. Reliable reference genes for normalizing quantitative gene expression data have not been carefully investigated. In this study, the suitability of a set of 15 apple genes were evaluated for their potential use as reliable reference genes. These genes were selected based on their low variance of gene expression in apple root tissues from a recent RNA-seq data set, and a few previously reported apple reference genes for other tissue types. Four methods, Delta Ct, geNorm, NormFinder and BestKeeper, were used to evaluate their stability in apple root tissues of various genotypes and under different experimental conditions. A small panel of stably expressed genes, MDP0000095375, MDP0000147424, MDP0000233640, MDP0000326399 and MDP0000173025 were recommended for normalizing quantitative gene expression data in apple roots under various abiotic or biotic stresses. When the most stable and least stable reference genes were used for data normalization, significant differences were observed on the expression patterns of two target genes, MdLecRLK5 (MDP0000228426, a gene encoding a lectin receptor like kinase and MdMAPK3 (MDP0000187103, a gene encoding a mitogen-activated protein kinase. Our data also indicated that for those carefully validated reference genes, a single reference gene is sufficient for reliable normalization of the quantitative gene expression. Depending on the experimental conditions, the most suitable reference genes can be specific to the sample of interest for more reliable RT-qPCR data normalization.

  14. Multiclass gene selection using Pareto-fronts.

    Science.gov (United States)

    Rajapakse, Jagath C; Mundra, Piyushkumar A

    2013-01-01

    Filter methods are often used for selection of genes in multiclass sample classification by using microarray data. Such techniques usually tend to bias toward a few classes that are easily distinguishable from other classes due to imbalances of strong features and sample sizes of different classes. It could therefore lead to selection of redundant genes while missing the relevant genes, leading to poor classification of tissue samples. In this manuscript, we propose to decompose multiclass ranking statistics into class-specific statistics and then use Pareto-front analysis for selection of genes. This alleviates the bias induced by class intrinsic characteristics of dominating classes. The use of Pareto-front analysis is demonstrated on two filter criteria commonly used for gene selection: F-score and KW-score. A significant improvement in classification performance and reduction in redundancy among top-ranked genes were achieved in experiments with both synthetic and real-benchmark data sets.

  15. Gene therapy in oral cancer: a review.

    Science.gov (United States)

    Kumar, M Sathish; Masthan, K M K; Babu, N Aravindha; Dash, Kailash Chandra

    2013-06-01

    Gene therapy is the use of DNA as an agent to treat disease. Gene therapy aims at the insertion of a functional gene into the cells of a patient for the correction of an inborn error of metabolism, to alter or repair an acquired genetic abnormality, and to provide new function to the cell. Many experiments have been done with respect to its application in various diseases.Today, most of the gene therapy studies are aimed at cancer and hereditary diseases which are linked to genetic defects. Cancer usually occurs due to the production of multiple mutations in a single cell which cause it to proliferate out of control. Several methods such as surgery, radiation therapy and chemotherapy have been used widely to treat cancers. But, the cancer patients who are not helped by these therapies can be treated by gene therapy. The purpose of this article is to review the use and purpose of gene therapy in oral cancer.

  16. Homeobox genes expressed during echinoderm arm regeneration.

    Science.gov (United States)

    Ben Khadra, Yousra; Said, Khaled; Thorndyke, Michael; Martinez, Pedro

    2014-04-01

    Regeneration in echinoderms has proved to be more amenable to study in the laboratory than the more classical vertebrate models, since the smaller genome size and the absence of multiple orthologs for different genes in echinoderms simplify the analysis of gene function during regeneration. In order to understand the role of homeobox-containing genes during arm regeneration in echinoderms, we isolated the complement of genes belonging to the Hox class that are expressed during this process in two major echinoderm groups: asteroids (Echinaster sepositus and Asterias rubens) and ophiuroids (Amphiura filiformis), both of which show an extraordinary capacity for regeneration. By exploiting the sequence conservation of the homeobox, putative orthologs of several Hox genes belonging to the anterior, medial, and posterior groups were isolated. We also report the isolation of a few Hox-like genes expressed in the same systems.

  17. Molecular targeting of gene therapy and radiotherapy

    International Nuclear Information System (INIS)

    Weichselbaum, R.R.; Kufe, D.W.; Advani, S.J.; Roizman, B.

    2001-01-01

    The full promise of gene therapy has been limited by the lack of specificity of vectors for tumor tissue as well as the lack of antitumor efficacy of transgenes encoded by gene delivery systems. In this paper we review our studies investigating two modifications of gene therapy combined with radiotherapy. The first investigations described include studies of radiation inducible gene therapy. In this paradigm, radio-inducible DNA sequences from the CarG elements of the Egr-1 promoter are cloned upstream of a cDNA encoding TNFa. The therapeutic gene (TNFa) is induced by radiation within the tumor microenvironment. In the second paradigm, genetically engineered herpes simplex virus (HSV-1) is induced by ionizing radiation to proliferate within the tumor volume. These modifications of radiotherapy and gene therapy may enhance the efficacy of both treatments

  18. Evaluating historical candidate genes for schizophrenia

    DEFF Research Database (Denmark)

    Farrell, M S; Werge, T; Sklar, P

    2015-01-01

    Prior to the genome-wide association era, candidate gene studies were a major approach in schizophrenia genetics. In this invited review, we consider the current status of 25 historical candidate genes for schizophrenia (for example, COMT, DISC1, DTNBP1 and NRG1). The initial study for 24...... of these genes explicitly evaluated common variant hypotheses about schizophrenia. Our evaluation included a meta-analysis of the candidate gene literature, incorporation of the results of the largest genomic study yet published for schizophrenia, ratings from informed researchers who have published...... on these genes, and ratings from 24 schizophrenia geneticists. On the basis of current empirical evidence and mostly consensual assessments of informed opinion, it appears that the historical candidate gene literature did not yield clear insights into the genetic basis of schizophrenia. A likely reason why...

  19. Expression Study of Banana Pathogenic Resistance Genes

    Directory of Open Access Journals (Sweden)

    Fenny M. Dwivany

    2016-10-01

    Full Text Available Banana is one of the world's most important trade commodities. However, infection of banana pathogenic fungi (Fusarium oxysporum race 4 is one of the major causes of decreasing production in Indonesia. Genetic engineering has become an alternative way to control this problem by isolating genes that involved in plant defense mechanism against pathogens. Two of the important genes are API5 and ChiI1, each gene encodes apoptosis inhibitory protein and chitinase enzymes. The purpose of this study was to study the expression of API5 and ChiI1 genes as candidate pathogenic resistance genes. The amplified fragments were then cloned, sequenced, and confirmed with in silico studies. Based on sequence analysis, it is showed that partial API5 gene has putative transactivation domain and ChiI1 has 9 chitinase family GH19 protein motifs. Data obtained from this study will contribute in banana genetic improvement.

  20. Gene bookmarking: keeping the pages open.

    Science.gov (United States)

    Sarge, Kevin D; Park-Sarge, Ok-Kyong

    2005-11-01

    'Gene bookmarking' is a mechanism of epigenetic memory that functions to transmit through mitosis the pattern of active genes and/or genes that can be activated to daughter cells. It is thought that, at a point before mitosis, genes that exist in an open, transcriptionally competent state are bound by proteins or marked by some kind of modification event. This is thought to facilitate the assembly of transcription complexes on the promoters in early G1, thereby ensuring that daughter cells have the same pattern of gene expression as the cell from which they derived. Little is known, however, about these 'bookmarking factors' and modifications or the mechanisms by which they mediate the transmission of transcriptional competence after mitosis is complete. Recent findings have provided new insights into the mechanisms, regulation and biological importance of gene bookmarking in eukaryotic cell function.