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Sample records for seed storage lipid

  1. Physalis peruviana seed storage

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    Cíntia L. M. de Souza

    2016-03-01

    Full Text Available ABSTRACT Physalis peruviana belongs to Solanaceae family and has a high nutritional and nutraceutical potential. The production is intended for fruit consumption and the propagation is mainly by seeds. This study aimed to evaluate the influence of priming on the kinetics of germination of P. peruviana seeds stored at different temperatures. The seeds were stored at 5 and 25 °C in a chamber saturated with zinc chloride solution and in liquid nitrogen (-196 °C. Every 4 months, the seeds were removed from storage for evaluation of germination and moisture content in the laboratory and emergence and development of seedlings in greenhouse. During the last evaluation at 16 months, the seeds under the same conditions were subjected to salt stress. The moisture content varied during the storage period, but was always higher for seeds kept at -196 ºC. These seeds kept high germination percentage in water until 16 months, regardless of the tested temperature; however, in salt solution the germination percentage was significantly reduced.

  2. Lysosomal lipid storage diseases.

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    Schulze, Heike; Sandhoff, Konrad

    2011-06-01

    Lysosomal lipid storage diseases, or lipidoses, are inherited metabolic disorders in which typically lipids accumulate in cells and tissues. Complex lipids, such as glycosphingolipids, are constitutively degraded within the endolysosomal system by soluble hydrolytic enzymes with the help of lipid binding proteins in a sequential manner. Because of a functionally impaired hydrolase or auxiliary protein, their lipid substrates cannot be degraded, accumulate in the lysosome, and slowly spread to other intracellular membranes. In Niemann-Pick type C disease, cholesterol transport is impaired and unesterified cholesterol accumulates in the late endosome. In most lysosomal lipid storage diseases, the accumulation of one or few lipids leads to the coprecipitation of other hydrophobic substances in the endolysosomal system, such as lipids and proteins, causing a "traffic jam." This can impair lysosomal function, such as delivery of nutrients through the endolysosomal system, leading to a state of cellular starvation. Therapeutic approaches are currently restricted to mild forms of diseases with significant residual catabolic activities and without brain involvement.

  3. Storage of sunflower seeds

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    Denise de Castro Lima

    Full Text Available The sunflower is among the top five crops in the world for the production of edible vegetable oil. The species displays rustic behavior, with an excellent edaphic and climatic adaptability index, being able to be cultivated throughout Brazil. Seed quality is the key to increasing production and productivity in the sunflower. The objective of this work was to monitor the viability of sunflower seeds with a view to their conservation when stored in different environments and packaging. The seeds were packed in paper bags, multilayered paper, black polyethylene and PET bottles; and stored for a period of twelve months in the following environments: dry cold room (10 ºC and 55% RH, the ambient conditions of Fortaleza, Ceará, Brazil (30-32 ºC and 75% RH, refrigerator (4 ºC and 38-43% RH and freezer (-20 ºC. Every three months, the water content of the seeds was determined and germination, accelerated ageing, speed of emergence index, and seedling dry weight were evaluated. The experimental design was completely randomized, in a scheme of split-lots, with four replications. It can be concluded that the natural environment is not suitable for the storage of sunflower seeds. Sunflower seeds remain viable for 12 months when stored in a dry cold room, refrigerator or freezer, irrespective of the type of packaging used.

  4. Lipid storage myopathies.

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    Bruno, Claudio; Dimauro, Salvatore

    2008-10-01

    The aim of this review is to provide an update on disorders of lipid metabolism affecting skeletal muscle exclusively or predominantly and to summarize recent clinical, genetic, and therapeutic studies in this field. Over the past 5 years, new clinical phenotypes and genetic loci have been described, unusual pathogenic mechanisms have been elucidated, and novel pharmacological approaches have been developed. At least one genetic defect responsible for the myopathic form of CoQ10 deficiency has been identified, causing a disorder that is allelic with the late-onset riboflavine-responsive form of multiple acyl-coenzyme A dehydrogenation deficiency. Novel mechanisms involved in the lipolytic breakdown of cellular lipid depots have been described and have led to the identification of genes and mutations responsible for multisystemic neutral lipid storage disorders, characterized by accumulation of triglyceride in multiple tissues, including muscle. Defects in lipid metabolism can affect either the mitochondrial transport and oxidation of exogenous fatty acid or the catabolism of endogenous triglycerides. These disorders impair energy production and almost invariably involve skeletal muscle, causing progressive myopathy with muscle weakness, or recurrent acute episodes of rhabdomyolysis triggered by exercise, fasting, or infections. Clinical and genetic characterization of these disorders has important implications both for accurate diagnostic approach and for development of therapeutic strategies.

  5. Lipids in citrus sinensis seeds

    International Nuclear Information System (INIS)

    Hamid, S.; Liaquat, L.; Khalid, B.; Khan, J.I.

    2003-01-01

    The seed oil of citrus sinensis when subjected to different physicochemical tests showed moisture 13.2%, ash 7.5%, ester value 1.29%, free fatty acid 0.4%. iodine value 65.0% and protein value 6.0%. According to lipid analysis. the oil was classified into hydrocarbons. wax esters, sterol esters, triglycerides. free fatty acids, 1,3 and 1,2 diglycerides, alcohols, sterols, monoglycerides, phosphatidylethanolamines, phosphatidylcholines and lysophosphatidylethanolamines. The fatty acid (C/sub 12.0/ - C/sub 21.0/) composition of all lipid classes was determined with the help of thin layer and gas liquid chromatography. (author)

  6. Neuroimaging of Lipid Storage Disorders

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    Rieger, Deborah; Auerbach, Sarah; Robinson, Paul; Gropman, Andrea

    2013-01-01

    Lipid storage diseases, also known as the lipidoses, are a group of inherited metabolic disorders in which there is lipid accumulation in various cell types, including the central nervous system, because of the deficiency of a variety of enzymes. Over time, excessive storage can cause permanent cellular and tissue damage. The brain is particularly…

  7. STORAGE OF Handroanthus umbellatus SEEDS

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    Cibele Chalita Martins

    2014-09-01

    Full Text Available http://dx.doi.org/10.5902/1980509815725Seed storage under controlled environmental conditions represents one of the most important lines of research to be applied on short-lived forest species as Handroanthus. The present research aimed to identify the most suitable seed storage conditions and longevity behavior of Handroanthus umbellatus seeds subject to the following storage treatments: packaging permeable paper bags under a no-controlled laboratory temperature and humidity (control and multiwall semipermeable bag at temperatures of -18 ºC, 1 ºC and 25 ºC. Seeds were dried to 6.3% of water content. Stored seeds were evaluated every three months until 24 months for water content, germination percentage and vigor utilizing first counting test. Seeds of T. umbellata are orthodox, with low longevity under natural conditions, once they remain viable for less than 5 months. The best conditions of seed preservation of these species were obtained by storage at -18° C in multiwall bags. Under these conditions physiological seed quality remains unchanged for a 24-month period.

  8. Storage requirements for sugar maple seeds

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    Harry W. Yawney; Clayton M., Jr. Carl

    1974-01-01

    Sugar maple seeds, collected from three trees in northern Vermont, were stored at four temperatures (18, 7, 2, and -10ºC) in combination with four seed moisture contents (35, 25, 17, and 10 percent). Seed moisture content and storage temperature significantly affected keeping ability, and these factors were highly interrelated. Seeds from all trees kept best...

  9. Effects of seed collecting date and storage duration on seed ...

    African Journals Online (AJOL)

    The objective of this study was to determine the effect of seed collecting dates (5 to 6 times from mid-November to early January, 10 days intervals) and seed storage duration (4, 8, and 12 months) at room temperature on seed germination of four Artemisia species (Artemisia sieberi, A. diffusa, A. kupetdaghensis, and A.

  10. Defatted Detarium senegalense seed-based diet alters lipid profile ...

    African Journals Online (AJOL)

    Defatted Detarium senegalense seed-based diet alters lipid profile, ... cheaper alternative source for good quality protein for dietary purposes, we evaluated Detarium ... Whole seed residue, DDS seed flour and control diets (soybeans) were ...

  11. Viability and Biochemical Content Changes in Seed Storage of Jabon Putih (Anthocephalus Cadamba (Roxb Miq.

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    Naning Yuniarti

    2015-08-01

    Full Text Available Seed deterioration is the process of deteriorated seed in view of viability that changed the physiological and chemical content. The aim of this research is to find out the effect of moisture content and storage on viability and biochemical content of jabon putih seed. Completely Randomized Design was used for decreasing moisture content based on seed drying time for 0, 24, 48, 72, 96, 120 hours, and Completely Randomized Factorial Design was used for the combination of moisture content (drying time for 0, 24, 48, 72, 96, 120 hours and room storage (ambient room, air-conditioned room, refrigerator. The results of this research are: (1 seed drying time and seed storage affected the change of seed viability and biochemical content, (2 seed drying time and seed storage influence significantly the value of moisture content, germination percentage, and biochemical content (lipid, carbohydrate, protein, (3 The longer time of seed drying and seed storage has decreased the seed moisture content, germination percentage, and the carbohydrate content, but it has increased the content of lipid and protein, and (4 the seeds were stored in refrigerator has better viability compared to dry cold storage and ambient room.

  12. Lipid peroxidation and seed emergency in progenies of the yellow passion fruit plant

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    João Paulo Bestete de Oliveira

    2012-09-01

    Full Text Available The objective was to evaluate the percentage of emergency plantlets and lipid peroxidation in seeds of 29 half-sib progenies of yellow passion fruit (Passiflora edulis Sims. after 24 months under storage. The experimental design was completely randomized, with four replications of 50 seeds each, from which the treatments were the progenies (1-29. The evaluation of the percent plantlet emergency was accomplished at 14 and 28 days after sowing. The lipid peroxidation of the seeds was expressed as malondialdehyde (MDA content that was determined by the TBARS method. Approximately 21% of those half-sib progenies maintained the viability of their seeds for twenty-four months under storage. The results point out a remarkable genetic variability for vigor and emergency of the yellow passion fruit plantlets, with occurrence of individuals with high and other ones with low capacity to maintaining the physiologic quality of their seeds after storage.

  13. Analysis of lipid profile in lipid storage myopathy.

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    Aguennouz, M'hammed; Beccaria, Marco; Purcaro, Giorgia; Oteri, Marianna; Micalizzi, Giuseppe; Musumesci, Olimpia; Ciranni, Annmaria; Di Giorgio, Rosa Maria; Toscano, Antonio; Dugo, Paola; Mondello, Luigi

    2016-09-01

    Lipid dysmetabolism disease is a condition in which lipids are stored abnormally in organs and tissues throughout the body, causing muscle weakness (myopathy). Usually, the diagnosis of this disease and its characterization goes through dosage of Acyl CoA in plasma accompanied with evidence of droplets of intra-fibrils lipids in the patient muscle biopsy. However, to understand the pathophysiological mechanisms of lipid storage diseases, it is useful to identify the nature of lipids deposited in muscle fiber. In this work fatty acids and triglycerides profile of lipid accumulated in the muscle of people suffering from myopathies syndromes was characterized. In particular, the analyses were carried out on the muscle biopsy of people afflicted by lipid storage myopathy, such as multiple acyl-coenzyme A dehydrogenase deficiency, and neutral lipid storage disease with myopathy, and by the intramitochondrial lipid storage dysfunctions, such as deficiencies of carnitine palmitoyltransferase II enzyme. A single step extraction and derivatization procedure was applied to analyze fatty acids from muscle tissues by gas chromatography with a flame ionization detector and with an electronic impact mass spectrometer. Triglycerides, extracted by using n-hexane, were analyzed by high performance liquid chromatography coupled to mass spectrometer equipped with an atmospheric pressure chemical ionization interface. The most representative fatty acids in all samples were: C16:0 in the 13-24% range, C18:1n9 in the 20-52% range, and C18:2n6 in the 10-25% range. These fatty acids were part of the most representative triglycerides in all samples. The data obtained was statistically elaborated performing a principal component analysis. A satisfactory discrimination was obtained among the different diseases. Using component 1 vs component 3 a 43.3% of total variance was explained. Such results suggest the important role that lipid profile characterization can have in supporting a correct

  14. In vitro storage of synthetic seeds: Effect of different storage ...

    African Journals Online (AJOL)

    In vitro derived shoots of olive cv. Moraiolo were employed in synthetic seeds preparation by alginate encapsulation, and then stored in artificial endosperm solution at cold (4°C) and room storage (21 ± 2°C) conditions in interaction with different storage intervals of 0, 15, 30, 45 and 60 days to evaluate the comparative ...

  15. Extraction and classification of lipids from seeds of persea ...

    African Journals Online (AJOL)

    Seed lipids of Persea americana and Chrysophyllum albidum were studied. Lipids were extracted with chloroform-methanol, analysed with silicic column chromatography and thin layer chromatography. The total lipid content of Persea americana was 10.8% while that of Chrysophyllum albidum was 7.7%. Fractionation of ...

  16. Lysosomal exocytosis and lipid storage disorders.

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    Samie, Mohammad Ali; Xu, Haoxing

    2014-06-01

    Lysosomes are acidic compartments in mammalian cells that are primarily responsible for the breakdown of endocytic and autophagic substrates such as membranes, proteins, and lipids into their basic building blocks. Lysosomal storage diseases (LSDs) are a group of metabolic disorders caused by genetic mutations in lysosomal hydrolases required for catabolic degradation, mutations in lysosomal membrane proteins important for catabolite export or membrane trafficking, or mutations in nonlysosomal proteins indirectly affecting these lysosomal functions. A hallmark feature of LSDs is the primary and secondary excessive accumulation of undigested lipids in the lysosome, which causes lysosomal dysfunction and cell death, and subsequently pathological symptoms in various tissues and organs. There are more than 60 types of LSDs, but an effective therapeutic strategy is still lacking for most of them. Several recent in vitro and in vivo studies suggest that induction of lysosomal exocytosis could effectively reduce the accumulation of the storage materials. Meanwhile, the molecular machinery and regulatory mechanisms for lysosomal exocytosis are beginning to be revealed. In this paper, we first discuss these recent developments with the focus on the functional interactions between lipid storage and lysosomal exocytosis. We then discuss whether lysosomal exocytosis can be manipulated to correct lysosomal and cellular dysfunction caused by excessive lipid storage, providing a potentially general therapeutic approach for LSDs. Copyright © 2014 by the American Society for Biochemistry and Molecular Biology, Inc.

  17. Lysosomal exocytosis and lipid storage disorders

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    Samie, Mohammad Ali; Xu, Haoxing

    2014-01-01

    Lysosomes are acidic compartments in mammalian cells that are primarily responsible for the breakdown of endocytic and autophagic substrates such as membranes, proteins, and lipids into their basic building blocks. Lysosomal storage diseases (LSDs) are a group of metabolic disorders caused by genetic mutations in lysosomal hydrolases required for catabolic degradation, mutations in lysosomal membrane proteins important for catabolite export or membrane trafficking, or mutations in nonlysosomal proteins indirectly affecting these lysosomal functions. A hallmark feature of LSDs is the primary and secondary excessive accumulation of undigested lipids in the lysosome, which causes lysosomal dysfunction and cell death, and subsequently pathological symptoms in various tissues and organs. There are more than 60 types of LSDs, but an effective therapeutic strategy is still lacking for most of them. Several recent in vitro and in vivo studies suggest that induction of lysosomal exocytosis could effectively reduce the accumulation of the storage materials. Meanwhile, the molecular machinery and regulatory mechanisms for lysosomal exocytosis are beginning to be revealed. In this paper, we first discuss these recent developments with the focus on the functional interactions between lipid storage and lysosomal exocytosis. We then discuss whether lysosomal exocytosis can be manipulated to correct lysosomal and cellular dysfunction caused by excessive lipid storage, providing a potentially general therapeutic approach for LSDs. PMID:24668941

  18. Autophagy, lipophagy and lysosomal lipid storage disorders.

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    Ward, Carl; Martinez-Lopez, Nuria; Otten, Elsje G; Carroll, Bernadette; Maetzel, Dorothea; Singh, Rajat; Sarkar, Sovan; Korolchuk, Viktor I

    2016-04-01

    Autophagy is a catabolic process with an essential function in the maintenance of cellular and tissue homeostasis. It is primarily recognised for its role in the degradation of dysfunctional proteins and unwanted organelles, however in recent years the range of autophagy substrates has also been extended to lipids. Degradation of lipids via autophagy is termed lipophagy. The ability of autophagy to contribute to the maintenance of lipo-homeostasis becomes particularly relevant in the context of genetic lysosomal storage disorders where perturbations of autophagic flux have been suggested to contribute to the disease aetiology. Here we review recent discoveries of the molecular mechanisms mediating lipid turnover by the autophagy pathways. We further focus on the relevance of autophagy, and specifically lipophagy, to the disease mechanisms. Moreover, autophagy is also discussed as a potential therapeutic target in several key lysosomal storage disorders. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  19. Ageing mechanisms in chickpea seeds: Relationship of sugar hydrolysis and lipid peroxidation with Amadori and Millard reactions

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    mahdi shaaban

    2017-05-01

    Full Text Available This experiment was performed in order to study on ageing mechanisms of chickpea seeds (Cicer arietinum L. in natural storage and accelerated ageing conditions in seed laboratory of Gorgan Agricultural Science and Natural Resources, Gorgan, Iran at 2015. Experiment was in completely randomized design arrangement with four replications. Treatments were 2 and 4 years natural storage and 1-5 days of accelerated ageing with control treatment. The results showed that with increasing of natural storage and accelerated ageing duration, germination percentage was decreased. Increasing of ageing duration decreased soluble sugars, non-reducing sugars and soluble proteins but lipid peroxidation, reducing sugars, protein carbonylation and Amadori and Millard reaction were increased. In natural storage condition lipid peroxidation was more than sugar hydrolysis but in accelerated ageing condition sugar hydrolysis was more than lipid peroxidation. These results show that the main reason of Amadori and Millard reaction in chickpea seeds in natural storage condition is lipid peroxidation and in accelerated ageing condition is sugar hydrolysis. Also, the results showed that Amadori reaction in natural storage condition was more than Amadori reaction and in accelerated ageing condition Millard reaction was more than Amadori reaction. The results of the present study showed that sever Millard reaction after Amadori reaction induced higher damage on seed and results to more decrease of seed viability and reduce of seed germination percentage in accelerated ageing than natural storage.

  20. Enhanced seed viability and lipid compositional changes during natural aging by suppressing phospholipase Dα in soybean seed

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    Lee, Junghoon; Welti, Ruth; Roth, Mary; Schapaugh, William T.; Li, Jiarui; Trick, Harold N.

    2013-01-01

    Summary Changes in phospholipid composition and consequent loss of membrane integrity are correlated with loss of seed viability. Furthermore, phospholipid compositional changes affect the composition of the triacylglycerols, i.e. the storage lipids. Phospholipase D (PLD) catalyzes the hydrolysis of phospholipids to phosphatidic acid, and PLDα is an abundant PLD isoform. Although wild type seeds stored for 33 months were non-viable, 30 to 50% of PLDα-knockdown (PLD-KD) soybean seeds stored for 33 months germinated. Wild type and PLD-KD seeds increased in lysophospholipid levels and in triacylglycerol fatty acid unsaturation during aging, but the levels of lysophospholipids increased more in wild type than in PLD-KD seeds. The loss of viability of wild type seeds was correlated with alterations in ultrastructure, including detachment of the plasma membrane from the cell wall complex and disorganization of oil bodies. The data demonstrate that, during natural aging, PLDα affects the soybean phospholipid profile and the triacylglycerol profile. Suppression of PLD activity in soybean seed has potential for improving seed quality during long-term storage. PMID:21895945

  1. Enhanced seed viability and lipid compositional changes during natural ageing by suppressing phospholipase Dα in soybean seed.

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    Lee, Junghoon; Welti, Ruth; Roth, Mary; Schapaugh, William T; Li, Jiarui; Trick, Harold N

    2012-02-01

    Changes in phospholipid composition and consequent loss of membrane integrity are correlated with loss of seed viability. Furthermore, phospholipid compositional changes affect the composition of the triacylglycerols (TAG), i.e. the storage lipids. Phospholipase D (PLD) catalyses the hydrolysis of phospholipids to phosphatidic acid, and PLDα is an abundant PLD isoform. Although wild-type (WT) seeds stored for 33 months were non-viable, 30%-50% of PLDα-knockdown (PLD-KD) soybean seeds stored for 33 months germinated. WT and PLD-KD seeds increased in lysophospholipid levels and in TAG fatty acid unsaturation during ageing, but the levels of lysophospholipids increased more in WT than in PLD-KD seeds. The loss of viability of WT seeds was correlated with alterations in ultrastructure, including detachment of the plasma membrane from the cell wall complex and disorganization of oil bodies. The data demonstrate that, during natural ageing, PLDα affects the soybean phospholipid profile and the TAG profile. Suppression of PLD activity in soybean seed has potential for improving seed quality during long-term storage. © 2011 The Authors. Plant Biotechnology Journal © 2011 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.

  2. Physicochemical Properties and Oxidative Storage Stability of Milled Roselle (Hibiscus sabdariffa L. Seeds

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    Nurul Hanisah Juhari

    2018-02-01

    Full Text Available Milled Roselle (Hibiscus sabdariffa L. seeds of the UMKL cultivar were analyzed for proximate composition, water and oil absorption capacity, and the influence of storage conditions on storage stability. The storage stability was determined under four types of conditions: light/oxygen (air (LO, light/nitrogen (LN, darkness/oxygen (air (DO, and darkness/nitrogen (DN while monitoring for seven consecutive months. During the storage period, the formation of volatiles was determined using dynamic headspace sampling and Gas Chromatography-Mass Spectrometry (GC-MS analysis. In total, 85 volatiles were identified, mainly aldehydes, alcohols, ketones, furans, and acids indicating lipid oxidation. It is recommended that milled Roselle seeds should be flushed with nitrogen and stored in darkness. Under these conditions, the seeds can be stored for at least three months without changes in volatile profile. This is important to ensure the good quality of milled Roselle seeds for further commercialization.

  3. Physicochemical Properties and Oxidative Storage Stability of Milled Roselle (Hibiscus sabdariffa L.) Seeds.

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    Juhari, Nurul Hanisah; Petersen, Mikael Agerlin

    2018-02-11

    Milled Roselle ( Hibiscus sabdariffa L.) seeds of the UMKL cultivar were analyzed for proximate composition, water and oil absorption capacity, and the influence of storage conditions on storage stability. The storage stability was determined under four types of conditions: light/oxygen (air) (LO), light/nitrogen (LN), darkness/oxygen (air) (DO), and darkness/nitrogen (DN) while monitoring for seven consecutive months. During the storage period, the formation of volatiles was determined using dynamic headspace sampling and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. In total, 85 volatiles were identified, mainly aldehydes, alcohols, ketones, furans, and acids indicating lipid oxidation. It is recommended that milled Roselle seeds should be flushed with nitrogen and stored in darkness. Under these conditions, the seeds can be stored for at least three months without changes in volatile profile. This is important to ensure the good quality of milled Roselle seeds for further commercialization.

  4. Incidence of storage fungi and hydropriming on soybean seeds

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    Costa,Denis Santiago da; Bonassa,Nathalie; Novembre,Ana Dionisia da Luz Coelho

    2013-01-01

    Priming is a technique applicable to seeds of various plant species; however, for soybean seed there is little available information correlating such technique to the storage fungi. The objective of this study was to assess hydropriming on soybeans seeds and correlate this technique to occurrence of such fungi. For this, soon after acquisition the soybean seeds, cv. M-SOY 7908 RR, were characterized by: moisture content, mechanical damage, viability (seed germination and seedling emergence) a...

  5. Producing the target seed: Seed collection, treatment, and storage

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    Robert P. Karrfalt

    2011-01-01

    The role of high quality seeds in producing target seedlings is reviewed. Basic seed handling and upgrading techniques are summarized. Current advances in seed science and technology as well as those on the horizon are discussed.

  6. Seed quality preservation advantage of gamma irradiation seed pre-treatment during long term storage in soybean

    International Nuclear Information System (INIS)

    Guha, Sameer Kumar; Sumedha; Singh, Bhupinder

    2014-01-01

    The experiment was conducted to analyse the effect of gamma irradiation seed pre-treatment on insect damage and quality preservation of soybean seeds during long term storage at ambient temperature (27-42 ℃) and relative humidity (50-95%). Freshly harvested seeds of soybean (cv. Pusa-9814) were treated with control (0), 0.01, 0.05, 0.5, 1.0, 3.0, 5.0 kGy gamma radiation on a Gamma irradiator (Gamma Chamber 5000, 60 Co source, activity 12000 Ci, BRIT, Mumbai, India) at the Nuclear Research Laboratory, IARI, New Delhi and were stored over one year in cotton cloth bags under ambient conditions. Protein and oil per cent and fatty acid profile was measured in freshly harvested zero time unirradiated control, aged unirradiated control and other treatments of ionizing radiation. Seed oil was extracted through the soxhlet extraction method and oil profiling was done by gas chromatography. Change in saturated and unsaturated fatty acids like palmitic, stearic, oleic, linolic, linolenic acid and oleic to linoleic ratio was measured. Oil content of unirradiated stored seeds compared to that of the freshly harvested control was lower. However, radiation in general, helped in maintaining a higher seed oil during storage when compared with that of the aged unirradiated control and was insignificantly reduced over the fresh unirrradiated control. Further, gamma irradiation treatment did not yield any adverse affect on the seed protein even after prolonged storage. The results reveal a reduced rate of lipid degradation and improved seed hardness over untreated control with no significant change in fatty acid profile of the irradiated and the unirradiated seeds over a long term storage period. (author)

  7. Chemical properties of Aspergillus flavus-infected soybean seeds exposed to gamma-irradiation during storage

    International Nuclear Information System (INIS)

    Mahrous, S.R.

    2007-01-01

    The aim of the present study was to examine the chemical properties of Aspergillus flavus-infected soybean seeds exposed to different levels of gamma-irradiation; 0 1, 3 and 5 kGy, during storage. The results revealed that there was no effect of irradiation at different dose levels on moisture, protein, total lipids and amino acids content of the seeds for overall 60 days of storage under ambient temperature. At zero time, irradiation of A. flavus- infected-soybean seeds at 5.0 kGy caused a slight increase in peroxide value, no change in acid value, a slight decrease in saponification and iodine values in the crude oil extracted from the seeds. An increase in saturated fatty acids associated with a decrease in un-saturated fatty acids was also observed in the oil extracted from the seeds. Furthermore, at dose level 5 kGy the fungus growth was completely inhibited and there was no detection of aflatoxin B1 after 60 days of storage. It is concluded that gamma-irradiation of A. flavus-infected soybean seeds at dose level 5 kGY is sufficient to inhibit fungus growth and aflatoxin production over a storage period of 60 days without changes in major chemical properties of the seeds and the oil extracted from seeds

  8. Storage Potential of Local Brazilian Pine Seed Varieties

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    Cristhyane Garcia Araldi

    2018-03-01

    Full Text Available ABSTRACT Brazilian pine seeds (Araucaria angustifolia are recalcitrant, and there are no studies evaluating the longevity of their different varieties. Our objective was to evaluate the capacity of different varieties of Brazilian pine seeds to maintain their physiological quality during storage. Seeds of the varieties: sancti josephi (I, angustifolia (II, caiova (III and indehiscens (IV were collected from two populations located in Santa Catarina, and stored under laboratory conditions and in a cold room for 90 days. On average, freshly harvested seeds showed 88% viability, and varieties II and III maintained the greatest viability (with the greatest vigor for variety II after 90 days in storage. Varieties I and II maintained their pre-germinative metabolism for a longer period than the other varieties during storage. Therefore, seeds from the angustifolia variety (II have higher storage potential than the other varieties, maintaining approximately 61% viability at 90 days of storage.

  9. Radiation pre-treatment of seed imparts metabolic stability and quality protection during storage under accelerated aging

    International Nuclear Information System (INIS)

    Sumedha; Singh, Bhupinder; Singhal, R.K.

    2014-01-01

    Quality of seed is known to deteriorate due to unfavoured temperature and relative humidity during accelerated aging. Protecting the seed quality during storage is thus a priority challenge for the seed industry till the seeds end up with the end user. Gamma radiation treatment has been shown to improve source to sink transport of photosynthates during terminal heat stress in cereals. An experiment was conducted to measure seed irradiation induced biochemical changes in groundnut cultivar TG-37A stored under accelerated aging at 45±2 ℃ and 95-100 % humidity for 0, 5, 10, 15, 20 and 40 days to aging. The gamma irradiation doses were control (0), 0.005, 0.025, 0.1, 0.5 and 1.0 kGy. Seed samples were periodically evaluated for oil and fatty acid profile by the soxhlet extraction method and gas chromatography mass spectroscopy respectively. Here alike earlier studies variation in fatty acid profile of seed was used as an indicator of seed health under harsh storage conditions of accelerated aging. In the majority of plant species having oil rich seeds, the lipids that are at risk of auto-oxidation contain oleic (18:1), linoleic (18:2) and linolenic (18:3) fatty acid chain. Degree of unsaturation has a significant influence on degree of degradation. Decreased total lipid content in aged sunflower seeds have been reported. Auto-oxidation of unsaturated fatty acids was noticed, especially for linoleic acid in seed of oil crop after seven years of storage. Our results showed radiation treatment did not cause any significant change in oleic (C18:1), linoleic acids (C18:2) and linolenic (C18:3) acid content of seeds. This shows that gamma radiation essentially slows down the lipid peroxidation which essentially protects membrane permeability and reduces the rate of seed deterioration at high temperature and RH. Radiation treatment imparts metabolic stability in seeds during storage under accelerated aging conditions. (author)

  10. Novel lipid constituents identified in seeds of Nigella sativa (Linn)

    International Nuclear Information System (INIS)

    Mehta, B.K.; Verma, Manjul; Gupta, Meenal

    2008-01-01

    Novel lipids were isolated from the unsaponifiable matter extracted from seeds of Nigella sativa Linn by using n-hexane. The new dienoate and two monoesters were the new lipids identified by spectral (IR, 1 H- and 13 C-NMR spectra, mass spectrum, elemental analysis) and chemical analysis. The dienoate (1) was identified as methylnonadeca-15,17-dienoate and two monoesters were identified as pentyl hexadec-12-enoate (2) and pentyl pentadec-11-enoate (3). Linoleic acid, oleic acid, β-sitosterol and stigmasterol were identified as part of the lipid structures. All compounds exhibited moderate activity against Staphylococcus aureus and poor activity against shigella spp, and Klebsiella pneumoniae. (author)

  11. IMPORTANCE OF STORAGE CONDITIONS AND SEED TREATMENT FOR SUNFLOWER HYBRIDS SEEDS GERMINATION

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    Goran Krizmanić

    2014-12-01

    Full Text Available In this research we have determined germination energy and germination of seeds of sunflower hybrids ‘Luka’ and ‘Apolon’, at the beginning of storage and 6, 12 and 18 months after of storage period (2011-2012 in the floor concrete storage at two different air temperatures and humidity (S-1: air temperature 15-18°C and relative air humidity 65-70% as well as in climate chamber (S-2: air temperature 10-12°C and relative air humidity 60-65%, stored in four treatments (Control: processed-untreated seed; T-1: treated with A.I. metalaxyl-M; T-2: treated with A.I. metalaxyl-M + A.I. imidacloprid and T-3: treated with A.I. metalaxyl-M + A.I. clothianidin. Based on the obtained results we have determined that sunflower hybrid ‘Luka’, compared to hybrid ‘Apolon’, in the given storage conditions and with the same seed treatment has 5-8% higher germination energy and seed germination and that in climate chamber both hybrids have 5-7% higher germination energy. Seed treatment of both sunflower hybrids with A.I. imidacloprid maximally reduced initial germination energy and seed germination in all tested periods and conditions of storage. On the average, natural seed, after 18 months of storage did not have better seed quality compared to seed treated with A.I. metalaxyl-M while other treatments had more significant influence on reduction of germination energy and seed germination, 6-15%. On the average, compared to other variants, seeds treated with A.I. metalaxyl-M after 18 months of storage in both storage conditions had higher germination energy by 4-15%, and seed germination by 2-12%.

  12. Engineering of soybean seed storage proteins

    International Nuclear Information System (INIS)

    Dickinson, C.D.; Floener, L.A.; Evans, R.P.; Nielsen, N.C.

    1987-01-01

    Protein engineering is one approach to the improvement of seed quality. With this in mind, a rapid in vitro system has been developed to assay the effect structural modifications have on the assembly of glycinin and β-conglycinin subunit complexes. Transcription plasmids were constructed for production of synthetic glycinin and β-conglycinin mRNAs by SP6 RNA-polymerase. Radiolabeled translation products from these messages were tested for their ability to form complexes. Gy4 and Gy5 proglycinins (group-2 subunits) and the a-subunit of β-conglycinin self-assembled into trimers. Proglycinin Gy2 (group-1 subunit) did not self-assemble, but assembled into mixed trimers in combination with Gy4 proglycinin. No assembly was observed for preproglycinins Gyl and Gy4, or for a Gy4 proglycinin which lacked 27 amino acids in a highly conserved internal sequence. Insertion of alternating MET-ARG residues in predicted turn regions of a hypervariable sequence in Gy4 proglycinin were tolerated when the string was short but inhibited trimer assembly as it became longer. The response to several different long deletions in this hypervariable region have also been tested. Different levels of trimer assembly were obtained and may depend on the secondary structures of the regions being joined in the engineered subunits. This system will be useful to study the assembly of storage protein complexes and to screen against modifications that interfere with subunit assembly

  13. A role for seed storage proteins in Arabidopsis seed longevity

    NARCIS (Netherlands)

    Nguyen, Thu-Phuong|info:eu-repo/dai/nl/328228818; Cueff, Gwendal; Hegedus, Dwayne D; Rajjou, Loïc; Bentsink, Leónie|info:eu-repo/dai/nl/241338735

    2015-01-01

    Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins and identifying the molecular mechanisms that govern seed germination, vigour and viability in response to ageing. In this work the dry seed proteome of four Arabidopsis thaliana

  14. Physicochemical properties of Terminalia catappa seed oil as a novel dietary lipid source

    Directory of Open Access Journals (Sweden)

    Supatcha Janporn

    2015-06-01

    Full Text Available Terminalia catappa Linn (TC is an ornamental tree planted extensively in many countries. It has been known for a long time that the seeds are edible but no research has focused on the realm of its use as food. Our previous data showed that the seed contains high levels of oil content (600 g/kg and possesses the optimum fatty acid balance indicated in fat dietary guidelines. This study aims to investigate the physical and chemical properties and the possibility of using TC seed oil as a new dietary lipid. The effects of extraction conditions, partial refining process, and storage stability on TC oil properties were conducted compared with soybean oil. The results showed that physicochemical properties including the density, refractive index, melting point, acidity, free fatty acid, saponification value, unsaponifiable, peroxide, and fatty acid composition of the extracted oil were comparable with soybean oil and their values followed the dietary standard of edible oil.

  15. Novel lipid constituents identified in seeds of Nigella sativa (Linn)

    Energy Technology Data Exchange (ETDEWEB)

    Mehta, B.K.; Verma, Manjul; Gupta, Meenal [Vikram University (India). School of Studies in Chemistry and Biochemistry]. E-mail: bkmehta11@yahoo.com

    2008-07-01

    Novel lipids were isolated from the unsaponifiable matter extracted from seeds of Nigella sativa Linn by using n-hexane. The new dienoate and two monoesters were the new lipids identified by spectral (IR, {sup 1}H- and {sup 13}C-NMR spectra, mass spectrum, elemental analysis) and chemical analysis. The dienoate (1) was identified as methylnonadeca-15,17-dienoate and two monoesters were identified as pentyl hexadec-12-enoate (2) and pentyl pentadec-11-enoate (3). Linoleic acid, oleic acid, {beta}-sitosterol and stigmasterol were identified as part of the lipid structures. All compounds exhibited moderate activity against Staphylococcus aureus and poor activity against shigella spp, and Klebsiella pneumoniae. (author)

  16. Irradiation and storage effect on some characteristics of soy seeds

    International Nuclear Information System (INIS)

    Ramirez Ascheri, Diego Palmiro; Devilla, Ivano Alessandro

    2008-01-01

    The irradiation has been applied frequently in seeds conservation to obtain reduction of losses caused by physiologic processes, besides reducing the microbial load. However, the irradiation process for X-rays is not a common practice in seeds; for that, it is necessary to study that process, in order to know irradiation effect on the soy seeds quality. The objective of this work was to verify the irradiation effect of X-rays and the storage period on the water, oil and protein contends of soy seeds (Glycine max L.) variety Emgopa 302. The experiment was represented by the combination of two factors: X-rays dosage with four irradiation levels [0, 50, 65 and 70 kV] and storage period in laboratory atmosphere with five levels (0, 15, 30, 45 and 60 days). The water content, oil and protein in seeds were assayed in the beginning and every 15 days of storage. The results showed the seeds quality stayed unaffected in irradiation function in the beginning of the experience, with alterations after 15 days, was verified a quality decreasing with the increasing of the X-rays dosage. The soy seeds irradiated had reduced quality in elapsing of the storage period. (author)

  17. Genotypic variability and mutant identification in cicer arietinum L. by seed storage protein profiling

    International Nuclear Information System (INIS)

    Hameed, A.; Iqbal, N.; Shah, T.M.

    2012-01-01

    A collection of thirty-four chickpea genotypes, including five kabuli and twenty-nine desi, were analyzed by SDS-PAGE for seed storage protein profiling. Total soluble seed proteins were resolved on 12% gels. A low level of variability was observed in desi as compared to kabuli genotypes. Dendrogram based on electrophoretic data clustered the thirty-four genotypes in four major groups. As large number of desi genotypes illustrated identical profiles, therefore could not be differentiated on the basis of seed storage protein profiles. One kabuli genotype ILC-195 found to be the most divergent showing 86% similarity with all other genotypes. ILC-195 can be distinguished from its mutant i.e., CM-2000 and other kabuli genotypes on the basis of three peptides i.e. SSP-66, SSP-43 and SSP-39. Some proteins peptides were found to be genotype specific like SSP-26 for ICCV-92311. Uniprot and NCBI protein databases were searched for already reported and characterized seed storage proteins in chickpea. Among 33 observed peptides, only six seed storages proteins from chickpea source were available in databases. On the basis of molecular weight similarity, identified peptides were SSP-64 as Serine/Threonine dehydratase, SSP-56 as Alpha-amylase inhibitor, SSP-50 as Provicillin, SSP-39 as seed imbibition protein, SSP-35 as Isoflavane reductase and SSP-19 as lipid transport protein. Highest variability was observed in vicillin subunits and beta subunits of legumins and its polymorphic forms. In conclusion, seed storage profiling can be economically used to asses the genetic variation, phylogenetic relationship and as markers to differentiate mutants from their parents. (author)

  18. Surface sterilization and duration of seed storage influenced ...

    African Journals Online (AJOL)

    The effects of factorial combinations of four storage duration (in days after seed extraction) and surface sterilization with three dilution levels of sodium hypochlorite on seedling emergence and seedling quality of African breadfruit were studied. Storage duration significantly influenced days to seedling emergence, ...

  19. Proteomic analysis of oil bodies in mature Jatropha curcas seeds with different lipid content.

    Science.gov (United States)

    Liu, Hui; Wang, Cuiping; Chen, Fan; Shen, Shihua

    2015-01-15

    spots in the electrophoresis gels. Furthermore, the interaction of OB associated protein species and their contribution to OB formation and stabilization are still blank. In this study, with the overall object of profiling OB protein species from mature J. curcas seeds with different lipid content, we provided a setting of comparative OB proteomics with biochemical data and transient expression to explore the core of OB associated protein species involved in the regulation of OB size and lipid accumulation. The results were important for biotechnological improvement with the aim to a global modification of lipid storage in J. curcas seeds. Meanwhile, this study gave insight into possible associations between OBs and other organelles in mature J. curcas seeds. It may represent new aspects of the biological functions of the OBs during the oil mobilization. Combined the technique of transient transformation, a newly reported protein species, glycine-rich RNA binding protein (GRP) was successfully targeted in OBs. Therefore, further molecular analysis of these protein species is warranted to verify this association and what role they have in OBs. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. Symptomatic lipid storage in carriers for the PNPLA2 gene

    NARCIS (Netherlands)

    Janssen, M.C.H.; Engelen, B.G.M. van; Kapusta, L.; Lammens, M.M.; Dijk, M.; Fischer, J.; Graaf, M. van der; Wevers, R.A.; Fahrleitner, M.; Zimmermann, R.; Morava, E.

    2013-01-01

    Neutral lipid storage disease comprises a heterogeneous group of inherited disorders characterized by severe accumulation of cytoplasmic triglyceride droplets in several tissues and neutrophils. A novel type of autosomal recessive lipid myopathy due to PNPLA2 mutations was recently described with

  1. Exogenous glutamine increases lipid accumulation in developing seeds of castor bean (Ricinus communis L. cultured in vitro

    Directory of Open Access Journals (Sweden)

    Zhang Yang

    2015-01-01

    Full Text Available This report describes biomass production and compositional changes of developing castor seeds in response to change in the nitrogen resource (glutamine of the medium. During the early developmental period (24-36 days after pollination, oil was found to initially accumulate in the developing seeds. Carbohydrates and oil were inversely related after glutamine provision (35 mM, in the culture medium. [U-14C] sucrose labeling was used to investigate the effect of metabolic fluxes among different storage materials. Addition of glutamine led to a 7% increase of labeling in lipids and an inverse decrease of labeling in carbohydrates. It was postulated that changes in the glutamine concentration in the medium are likely to influence the partitioning of resources between the various storage products, especially carbohydrates and oil. These observations will contribute to a better understanding of assimilate partitioning in developing castor seeds and the development of molecular strategies to improve castor bean seed quality and plant breeding studies.

  2. The physiology of lipid storage and use in reptiles.

    Science.gov (United States)

    Price, Edwin R

    2017-08-01

    Lipid metabolism is central to understanding whole-animal energetics. Reptiles store most excess energy in lipid form, mobilise those lipids when needed to meet energetic demands, and invest lipids in eggs to provide the primary source of energy to developing embryos. Here, I review the mechanisms by which non-avian reptiles store, transport, and use lipids. Many aspects of lipid absorption, transport, and storage appear to be similar to birds, including the hepatic synthesis of lipids from glucose substrates, the transport of triglycerides in lipoproteins, and the storage of lipids in adipose tissue, although adipose tissue in non-avian reptiles is usually concentrated in abdominal fat bodies or the tail. Seasonal changes in fat stores suggest that lipid storage is primarily for reproduction in most species, rather than for maintenance during aphagic periods. The effects of fasting on plasma lipid metabolites can differ from mammals and birds due to the ability of non-avian reptiles to reduce their metabolism drastically during extended fasts. The effect of fasting on levels of plasma ketones is species specific: β-hydroxybutyrate concentration may rise or fall during fasting. I also describe the process by which the bulk of lipids are deposited into oocytes during vitellogenesis. Although this process is sometimes ascribed to vitellogenin-based transport in reptiles, the majority of lipid deposition occurs via triglycerides packaged in very-low-density lipoproteins (VLDLs), based on physiological, histological, biochemical, comparative, and genomic evidence. I also discuss the evidence for non-avian reptiles using 'yolk-targeted' VLDLs during vitellogenesis. The major physiological states - feeding, fasting, and vitellogenesis - have different effects on plasma lipid metabolites, and I discuss the possibilities and potential problems of using plasma metabolites to diagnose feeding condition in non-avian reptiles. © 2016 Cambridge Philosophical Society.

  3. Lipid droplets as ubiquitous fat storage organelles in C. elegans

    Directory of Open Access Journals (Sweden)

    Guo Fengli

    2010-12-01

    Full Text Available Abstract Background Lipid droplets are a class of eukaryotic cell organelles for storage of neutral fat such as triacylglycerol (TAG and cholesterol ester (CE. We and others have recently reported that lysosome-related organelles (LROs are not fat storage structures in the nematode C. elegans. We also reported the formation of enlarged lipid droplets in a class of peroxisomal fatty acid β-oxidation mutants. In the present study, we seek to provide further evidence on the organelle nature and biophysical properties of fat storage structures in wild-type and mutant C. elegans. Results In this study, we provide biochemical, histological and ultrastructural evidence of lipid droplets in wild-type and mutant C. elegans that lack lysosome related organelles (LROs. The formation of lipid droplets and the targeting of BODIPY fatty acid analogs to lipid droplets in live animals are not dependent on lysosomal trafficking or peroxisome dysfunction. However, the targeting of Nile Red to lipid droplets in live animals occurs only in mutants with defective peroxisomes. Nile Red labelled-lipid droplets are characterized by a fluorescence emission spectrum distinct from that of Nile Red labelled-LROs. Moreover, we show that the recently developed post-fix Nile Red staining method labels lipid droplets exclusively. Conclusions Our results demonstrate lipid droplets as ubiquitous fat storage organelles and provide a unified explanation for previous studies on fat labelling methods in C. elegans. These results have important applications to the studies of fat storage and lipid droplet regulation in the powerful genetic system, C. elegans.

  4. Refractory Hyperlactatemia with Organ Insufficiency in Lipid Storage Myopathy.

    Science.gov (United States)

    Xu, Yuanda; Zhou, Li; Liang, Weibo; He, Weiqun; Liu, Xiaoqing; Liang, Xiuling; Zhong, Nanshan; Li, Yimin

    2015-08-01

    Lipid storage myopathy is a metabolic disorder characterized by abnormal lipid accumulation in muscle fibers and progressive muscle weakness. Here, we report the case of a 17-year-old woman with progressive muscle weakness, refractory hyperlactatemia, and multiple organ insufficiency. Severe pneumonia was the initial diagnosis. After anti-infective treatment, fluid resuscitation, and mechanical ventilation, the patient's symptoms improved but hyperlactatemia and muscle weakness persisted. She was empirically treated with carnitine. Biochemical tests, electromyography, and muscle biopsy confirmed lipid storage myopathy. After 7 weeks of treatment, the patient resumed normal daily life. An empirical treatment with carnitine may be beneficial for patients before an accurate diagnosis of lipid storage myopathy is made.

  5. Stress, storage and survival of neem seed

    NARCIS (Netherlands)

    Sacandé, M.

    2000-01-01

    Neem ( Azadirachta indica ) is an important multipurpose tropical tree species, frequently used in planting programmes in the arid tropics. However, its seeds are difficult to store for extended periods of time, as are those of many other tropical species which display

  6. Expression of mouse MGAT in Arabidopsis results in increased lipid accumulation in seeds

    Directory of Open Access Journals (Sweden)

    Anna eEl Tahchy

    2015-12-01

    Full Text Available Worldwide demand for vegetable oil is projected to double within the next thirty years due to increasing food, fuel and industrial requirements. There is therefore great interest in metabolic engineering strategies that boost oil accumulation in plant tissues, however, efforts to date have only achieved levels of storage lipid accumulation in plant tissues far below the benchmark to meet demand. Monoacylglycerol acyltransferase (MGAT is predominantly associated with lipid absorption and resynthesis in the animal intestine where it catalyses monoacylglycerol (MAG to form diacylglycerol (DAG, and then triacylglycerol (TAG. In contrast plant lipid biosynthesis routes do not include MGAT. Rather, DAG and TAG are either synthesized from glycerol-3-phosphate (G-3-P by a series of three subsequent acylation reactions, or originate from phospholipids via an acyl editing pathway. Mouse MGATs 1 and 2 have been shown to increase oil content transiently in Nicotiana benthamiana leaf tissue by 2.6 fold. Here we explore the feasibility of this approach to increase TAG in Arabidopsis thaliana seed. The stable MGAT2 expression resulted in a significant increase in seed oil content by 1.32 fold. We also report evidence of the MGAT2 activity based on in vitro assays. Up to 3.9 fold increase of radiolabelled DAG were produced in seed lysate which suggest that the transgenic MGAT activity can result in DAG re-synthesis by salvaging the MAG product of lipid breakdown. The expression of MGAT2 therefore creates an independent and complementary TAG biosynthesis route to the endogenous Kennedy pathway and other glycerolipid synthesis routes.

  7. Symptomatic lipid storage in carriers for the PNPLA2 gene.

    Science.gov (United States)

    Janssen, Mirian C H; van Engelen, Baziel; Kapusta, Livia; Lammens, Martin; van Dijk, Martin; Fischer, Judith; van der Graaf, Marinette; Wevers, Ron A; Fahrleitner, Manuela; Zimmermann, Robert; Morava, Eva

    2013-08-01

    Neutral lipid storage disease comprises a heterogeneous group of inherited disorders characterized by severe accumulation of cytoplasmic triglyceride droplets in several tissues and neutrophils. A novel type of autosomal recessive lipid myopathy due to PNPLA2 mutations was recently described with associated cardiac disease, myopathy and frequent infections, but without ichthyosis. Here we describe the clinical and biochemical characteristics of a long surviving patient and report on four carrier family members with diverse clinical involvement. Interestingly, heterozygous patients show neutral lipid storage in muscle and in the keratocytes of the skin, Jordans' bodies, mild myopathy and frequent infections. Biochemical analysis of fibroblasts obtained from patients revealed increased triglyceride storage and reduced lipid droplet-associated triglyceride hydrolase activity. Together, our data implicate that the wild-type allele cannot fully compensate for the mutated dysfunctional allele of PNPLA2 leading to triglyceride accumulation in muscle and mild myopathy in PNPLA2 mutation carriers. The presence of neutral lipid droplets in the skin in PNPLA2 mutation carriers strengthens the link between NLSD and other neutral lipid storage diseases with ichthyosis.

  8. Effect of storage in overcoming seed dormancy of Annona coriacea Mart. seeds

    Directory of Open Access Journals (Sweden)

    DAIANE M. DRESCH

    2014-12-01

    Full Text Available The aim of this study was to evaluate the effect of pre-treatments on overcoming dormancy of A. coriacea seeds. Seeds were processed and stored in polyethylene bags at temperatures of at -18°C (42% RH, 5°C (34% RH, 15°C (60% RH and 25°C (34% RH, during 0, 30, 60, 90, 120 and 150 days. After storage, seeds were immersed in 350 mg.L−1 gibberellic acid for 144 hours. Sowing was carried out in plastic bags containing Red Latosol + Bioplant®. Moisture content, emergence percentage, emergence speed index, length and dry mass of seedlings, were evaluated. The experimental design was completely randomized in a factorial with four replications of 50 seeds each. The seed storage at 5°C and subsequent immersion in gibberellic acid was efficient to reach high percentage, emergence speed and plant growth. A. coriacea seeds showed non-deep simple morphophysiological dormancy wherein the physiological component can be overcome after the seeds are storage at 5°C for a maximum period of 53 days and subsequent immersed in exogenous GA (350 mg.L−1 for 144 hours.

  9. Effect of storage in overcoming seed dormancy of Annona coriacea Mart. seeds.

    Science.gov (United States)

    Dresch, Daiane M; Scalon, Silvana P Q; Masetto, Tathiana E

    2014-12-01

    The aim of this study was to evaluate the effect of pre-treatments on overcoming dormancy of A. coriacea seeds. Seeds were processed and stored in polyethylene bags at temperatures of at -18°C (42% RH), 5°C (34% RH), 15°C (60% RH) and 25°C (34% RH), during 0, 30, 60, 90, 120 and 150 days. After storage, seeds were immersed in 350 mg.L-1 gibberellic acid for 144 hours. Sowing was carried out in plastic bags containing Red Latosol + Bioplant®. Moisture content, emergence percentage, emergence speed index, length and dry mass of seedlings, were evaluated. The experimental design was completely randomized in a factorial with four replications of 50 seeds each. The seed storage at 5°C and subsequent immersion in gibberellic acid was efficient to reach high percentage, emergence speed and plant growth. A. coriacea seeds showed non-deep simple morphophysiological dormancy wherein the physiological component can be overcome after the seeds are storage at 5°C for a maximum period of 53 days and subsequent immersed in exogenous GA (350 mg.L-1 for 144 hours).

  10. In vitro storage of synthetic seeds: Effect of different storage ...

    African Journals Online (AJOL)

    GREGORY

    2010-08-30

    Aug 30, 2010 ... comparative regrowth and conversion capacity of synthetic seeds. Cold stored .... solution for 35 min. After complexation, the hardened alginate ... difference (LSD) test at 5% probability level (Steel et al., 1997). RESULTS AND ..... dure already proposed by Roussos and Pontikis (2002) that simplified the ...

  11. Skylarks trade size and energy content in weed seeds to maximize total ingested lipid biomass.

    Science.gov (United States)

    Gaba, Sabrina; Collas, Claire; Powolny, Thibaut; Bretagnolle, François; Bretagnolle, Vincent

    2014-10-01

    The trade-off between forage quality and quantity has been particularly studied in herbivore organisms, but much less for seed eating animals, in particular seed-eating birds which constitute the bulk of wintering passerines in European farmlands. The skylark is one of the commonest farmland birds in winter, mainly feeding on seeds. We focus on weed seeds for conservation and management purposes. Weed seeds form the bulk of the diet of skylarks during winter period, and although this is still a matter for discussion, weed seed predation by granivorous has been suggested as an alternative to herbicides used to regulate weed populations in arable crops. Our objectives were to identify whether weed seed traits govern foraging decisions of skylarks, and to characterize key seed traits with respect to size, which is related to searching and handling time, and lipid content, which is essential for migratory birds. We combined a single-offer experiment and a multiple-offer one to test for feeding preferences of the birds by estimating seed intake on weed seed species differing in their seed size and seed lipid content. Our results showed (1) a selective preference for smaller seeds above a threshold of seed size or seed size difference in the pair and, (2) a significant effect of seed lipid biomass suggesting a trade-off between foraging for smaller seeds and selecting seeds rich in lipids. Skylarks foraging decision thus seems to be mainly based on seed size, that is presumably a 'proxy' for weed seed energy content. However, there are clearly many possible combinations of morphological and physiological traits that must play crucial role in the plant-bird interaction such as toxic compound or seed coat. Copyright © 2014 Elsevier B.V. All rights reserved.

  12. Proteomic analysis of lettuce seed germination and thermoinhibition by sampling of individual seeds at germination and removal of storage proteins by polyethylene glycol fractionation.

    Science.gov (United States)

    Wang, Wei-Qing; Song, Bin-Yan; Deng, Zhi-Jun; Wang, Yue; Liu, Shu-Jun; Møller, Ian Max; Song, Song-Quan

    2015-04-01

    Germination and thermoinhibition in lettuce (Lactuca sativa 'Jianyexianfeng No. 1') seeds were investigated by a proteomic comparison among dry seeds, germinated seeds at 15°C, at 15°C after imbibition at 25°C for 48 h, or at 25°C in KNO3 (all sampled individually at germination), and ungerminated seeds at 25°C, a thermoinhibitory temperature. Before two-dimensional gel electrophoresis analysis, storage proteins (greater than 50% of total extractable protein) were removed by polyethylene glycol precipitation, which significantly improved the detection of less abundant proteins on two-dimensional gels. A total of 108 protein spots were identified to change more than 2-fold (Pseeds than in ungerminated 25°C seeds. Gene expression of 12 of those proteins correlated well with the protein accumulation. Methionine metabolism, ethylene production, lipid mobilization, cell elongation, and detoxification of aldehydes were revealed to be potentially related to lettuce seed germination and thermoinhibition. Accumulation of three proteins and expression of five genes participating in the mevalonate (MVA) pathway of isoprenoid biosynthesis correlated positively with seed germinability. Inhibition of this pathway by lovastatin delayed seed germination and increased the sensitivity of germination to abscisic acid. MVA pathway-derived products, cytokinins, partially reversed the lovastatin inhibition of germination and released seed thermoinhibition at 25°C. We conclude that the MVA pathway for isoprenoid biosynthesis is involved in lettuce seed germination and thermoinhibition. © 2015 American Society of Plant Biologists. All Rights Reserved.

  13. Lack of Globulin Synthesis during Seed Development Alters Accumulation of Seed Storage Proteins in Rice

    Directory of Open Access Journals (Sweden)

    Hye-Jung Lee

    2015-06-01

    Full Text Available The major seed storage proteins (SSPs in rice seeds have been classified into three types, glutelins, prolamins, and globulin, and the proportion of each SSP varies. It has been shown in rice mutants that when either glutelins or prolamins are defective, the expression of another type of SSP is promoted to counterbalance the deficit. However, we observed reduced abundances of glutelins and prolamins in dry seeds of a globulin-deficient rice mutant (Glb-RNAi, which was generated with RNA interference (RNAi-induced suppression of globulin expression. The expression of the prolamin and glutelin subfamily genes was reduced in the immature seeds of Glb-RNAi lines compared with those in wild type. A proteomic analysis of Glb-RNAi seeds showed that the reductions in glutelin and prolamin were conserved at the protein level. The decreased pattern in glutelin was also significant in the presence of a reductant, suggesting that the polymerization of the glutelin proteins via intramolecular disulfide bonds could be interrupted in Glb-RNAi seeds. We also observed aberrant and loosely packed structures in the storage organelles of Glb-RNAi seeds, which may be attributable to the reductions in SSPs. In this study, we evaluated the role of rice globulin in seed development, showing that a deficiency in globulin could comprehensively reduce the expression of other SSPs.

  14. Dorfman-Chanarin syndrome: A rare neutral lipid storage disease

    OpenAIRE

    Mitra Souvik; Samanta Moumita; Sarkar Mihir; Chatterjee Sukanta

    2010-01-01

    Dorfman-Chanarin syndrome is a rare neutral lipid storage disorder characterized by ichthyosis, lipid vacuolations in peripheral leucocytes, and multisystem involvement. It is an autosomal recessive disorder caused by mutations in the CGI-58 gene. A total of 42 cases have been reported worldwide till February 2009 out of which 4 have been previously reported from India. We report a case of a 20-month-old male with congenital ichthyosis, organomegaly, and bilateral cryptorchidism. Examination ...

  15. Cerebrotendinous xanthomatosis (a rare lipid storage disorder): a case report

    OpenAIRE

    Razi, Syed Mohd; Gupta, Abhinav Kumar; Gupta, Deepak Chand; Gutch, Manish; Gupta, Keshav Kumar; Usman, Syeda Iqra

    2016-01-01

    Background Cerebrotendinous xanthomatosis is a very rare autosomal recessive lipid storage disorder affecting bile acid biosynthesis. It is manifested by subtle neurological and non-neurological symptoms due to abnormal tissue lipid deposition. Diagnosis is usually delayed but early diagnosis and replacement therapy can prevent devastating neurological sequelae. Case presentation We present a case of a 25-year-old Asian Indian woman who presented with gait difficulty, fusiform swellings of bi...

  16. Seed storage at elevated partial pressure of oxygen, a fast method for analysing seed ageing under dry conditions

    NARCIS (Netherlands)

    Groot, S.P.C.; Surki, A.A.; Vos, de R.C.H.; Kodde, J.

    2012-01-01

    Background and Aims Despite differences in physiology between dry and relative moist seeds, seed ageing tests most often use a temperature and seed moisture level that are higher than during dry storage used in commercial practice and gene banks. This study aimed to test whether seed ageing under

  17. Seed storage protein components are associated with curled ...

    African Journals Online (AJOL)

    PRECIOUS

    2009-11-16

    Nov 16, 2009 ... analysis suggests that the two increased protein spots in mutants were ... The main objective of this work was to gain further understanding of the influence of curled cotyledon on the seed storage protein components in soybean by com- .... cotyledon formation during Arabidopsis embryogenesis: interaction.

  18. Seed storage at elevated partial pressure of oxygen, a fast method for analysing seed ageing under dry conditions

    Science.gov (United States)

    Groot, S. P. C.; Surki, A. A.; de Vos, R. C. H.; Kodde, J.

    2012-01-01

    Background and Aims Despite differences in physiology between dry and relative moist seeds, seed ageing tests most often use a temperature and seed moisture level that are higher than during dry storage used in commercial practice and gene banks. This study aimed to test whether seed ageing under dry conditions can be accelerated by storing under high-pressure oxygen. Methods Dry barley (Hordeum vulgare), cabbage (Brassica oleracea), lettuce (Lactuca sativa) and soybean (Glycine max) seeds were stored between 2 and 7 weeks in steel tanks under 18 MPa partial pressure of oxygen. Storage under high-pressure nitrogen gas or under ambient air pressure served as controls. The method was compared with storage at 45 °C after equilibration at 85 % relative humidity and long-term storage at the laboratory bench. Germination behaviour, seedling morphology and tocopherol levels were assessed. Key Results The ageing of the dry seeds was indeed accelerated by storing under high-pressure oxygen. The morphological ageing symptoms of the stored seeds resembled those observed after ageing under long-term dry storage conditions. Barley appeared more tolerant of this storage treatment compared with lettuce and soybean. Less-mature harvested cabbage seeds were more sensitive, as was the case for primed compared with non-primed lettuce seeds. Under high-pressure oxygen storage the tocopherol levels of dry seeds decreased, in a linear way with the decline in seed germination, but remained unchanged in seeds deteriorated during storage at 45 °C after equilibration at 85 % RH. Conclusions Seed storage under high-pressure oxygen offers a novel and relatively fast method to study the physiology and biochemistry of seed ageing at different seed moisture levels and temperatures, including those that are representative of the dry storage conditions as used in gene banks and commercial practice. PMID:22967856

  19. Seed storage at elevated partial pressure of oxygen, a fast method for analysing seed ageing under dry conditions.

    Science.gov (United States)

    Groot, S P C; Surki, A A; de Vos, R C H; Kodde, J

    2012-11-01

    Despite differences in physiology between dry and relative moist seeds, seed ageing tests most often use a temperature and seed moisture level that are higher than during dry storage used in commercial practice and gene banks. This study aimed to test whether seed ageing under dry conditions can be accelerated by storing under high-pressure oxygen. methods: Dry barley (Hordeum vulgare), cabbage (Brassica oleracea), lettuce (Lactuca sativa) and soybean (Glycine max) seeds were stored between 2 and 7 weeks in steel tanks under 18 MPa partial pressure of oxygen. Storage under high-pressure nitrogen gas or under ambient air pressure served as controls. The method was compared with storage at 45 °C after equilibration at 85 % relative humidity and long-term storage at the laboratory bench. Germination behaviour, seedling morphology and tocopherol levels were assessed. The ageing of the dry seeds was indeed accelerated by storing under high-pressure oxygen. The morphological ageing symptoms of the stored seeds resembled those observed after ageing under long-term dry storage conditions. Barley appeared more tolerant of this storage treatment compared with lettuce and soybean. Less-mature harvested cabbage seeds were more sensitive, as was the case for primed compared with non-primed lettuce seeds. Under high-pressure oxygen storage the tocopherol levels of dry seeds decreased, in a linear way with the decline in seed germination, but remained unchanged in seeds deteriorated during storage at 45 °C after equilibration at 85 % RH. Seed storage under high-pressure oxygen offers a novel and relatively fast method to study the physiology and biochemistry of seed ageing at different seed moisture levels and temperatures, including those that are representative of the dry storage conditions as used in gene banks and commercial practice.

  20. The classification of fatty acids of lipids from seeds of Persea ...

    African Journals Online (AJOL)

    PROMOTING ACCESS TO AFRICAN RESEARCH ... Gas liquid chromatographic analyses of Persea grattisima and ... as oil seeds and the fatty-acids of seed lipids could be potential sources of industrial oil. Keywords: Classification, fatty acids, GLC and Lipids. Full Text: EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  1. EFFECT OF FERTILIZER ELEMENTS ON LIPIDS ACCUMULATION AND FATTY ACIDS COMPOSITION OF PUMPKIN SEEDS

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    S. M. Nadezhkin

    2013-01-01

    Full Text Available Effect of organic and mineral fertilizers on pumpkin seeds lipids accumulation and their fatty acids com position is investigated. The influence of nutrition's composition on the seeds size, lipids content and concentration of polyunsaturated fatty acids was shown.

  2. Selected aspects of tiny vetch [Vicia hirsuta (L. Gray S.F.] seed ecology: generative reproduction and effects of seed maturity and seed storage on seed germination

    Directory of Open Access Journals (Sweden)

    Magdalena Kucewicz

    2012-12-01

    Full Text Available Vicia hirsuta (L. Gray S.F. (tiny vetch is a common and persistent segetal weed. Tiny vetch seeds and pods reach different stages of maturity during the crop harvest season. Some seeds that mature before cereal harvest are shed in the field and deposited in the soil seed bank, while others become incorporated into seed material. The objective of this study was to describe selected aspects of tiny vetch seed ecology: to determine the rate of individual reproduction of vetch plants growing in winter and spring grain crops and to evaluate the germination of seeds at different stages of maturity, subject to storage conditions. The seeds and pods of V. hirsuta were sorted according to their development stages at harvest and divided into two groups. The first group was stored under laboratory conditions for two months. In the autumn of the same year, the seeds were subjected to germination tests. The remaining seeds were stored in a storeroom, and were planted in soil in the spring. The germination rate was evaluated after 8 months of storage. Potential productivity (developed pods and flowers, fruit buds was higher in plants fruiting in winter wheat than in spring barley. Vetch plants produced around 17-26% more pods (including cracked, mature, greenish-brown and green pods and around 25% less buds in winter wheat than in spring barley. Immature seeds were characterized by the highest germination capacity. Following storage under laboratory conditions and stratification in soil, mature seeds germinated at a rate of several percent. After storage in a storeroom, seeds at all three development stages broke dormancy at a rate of 72- 75%. The high germination power of tiny vetch seeds stored in a storeroom indicates that this plant can be classified as an obligatory speirochoric weed species.

  3. Germination and storage of caranda seeds (Copernicia alba

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    Tathiana Elisa Masetto

    2012-12-01

    Full Text Available Caranda is a Brazilian native palm tree, belonging to Arecaceae family and occurring, predominan,t in the Brazilian Swampland. This work studied the germination and the caranda seeds storage behavior. The germination study was carried out in the temperatures of 25ºC and 30ºC in constant white light and the alternate temperature of 20/30ºC with 10 hours of darkness for the lowest temperature and 14 hours of light for the highest temperature, using paper and paper roll as substratum. At the end of test, the germination percentage, germination speed index, germination medium time and the primary root length were evaluated. After the seeds improvement, it was obtained two sub-samples destined for 30 days storage in two invironments: cold and dry chamber (16ºC/55% UR and freezer (-18ºC. The following tests, water content, germination, germination medium time and primary root length were evaluated. The caranda seeds germination in paper roll and on paper is favored by the temperature of 20/30ºC in paper roll and on paper and paper roll on 30ºC. The freezing and cold camera storage during 30 days are efficient to reduce the germination medium time of caranda seeds and to keep the germination percentage.

  4. Antioxidant effect of mango seed extract and butylated hydroxytoluene in bologna-type mortadella during storage

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    Ana Lúcia Fernandes Pereira

    2011-03-01

    Full Text Available The effects of mango seed extract (MSE and butylated hydroxytoluene (BHT on pH, lipid oxidation, and color of Bologna-type mortadella during refrigerated storage for 21 days were studied. Bologna-type mortadella samples were formulated to contain 0.1% MSE, 0.2% MSE, or 0.01% BHT. After 14 days of storage, the products containing MSE 0.1 or 0.2% had higher pH values than those containing BHT 0.01%. Lipid oxidation values increased with storage time but were not affected by the type of antioxidant. The highest values for color parameter L* were observed for mortadella containing BHT 0.01% after 7, 14, and 21 days of storage. Values for the color parameters a* and b* tended to decrease during mortadella storage. Products containing 0.1 or 0.2% MSE showed higher values for color parameter a* and lower values for color parameter b* compared to those containing 0.01% BHT. It can be concluded that MSE can be used in 0.1 or 0.2% levels in Bologna-type mortadella with similar or better antioxidant effects than those of BHT 0.01%.

  5. Dorfman-Chanarin syndrome: a rare neutral lipid storage disease.

    Science.gov (United States)

    Mitra, Souvik; Samanta, Moumita; Sarkar, Mihir; Chatterjee, Sukanta

    2010-01-01

    Dorfman-Chanarin syndrome is a rare neutral lipid storage disorder characterized by ichthyosis, lipid vacuolations in peripheral leucocytes, and multisystem involvement. It is an autosomal recessive disorder caused by mutations in the CGI-58 gene. A total of 42 cases have been reported worldwide till February 2009 out of which 4 have been previously reported from India. We report a case of a 20-month-old male with congenital ichthyosis, organomegaly, and bilateral cryptorchidism. Examination of the peripheral smear revealed lipid vacuoles in the leucocytes consistent with Jordan's anomaly, which was confirmed by transmission electron microscopy. Liver biopsy revealed micronodular cirrhosis with macrovesicular steatosis while skin biopsy showed ichthyosis vulgaris. Dorfman-Chanarin syndrome was diagnosed on the basis of clinical and laboratory criteria with certain unreported manifestations. Dietary modifications were instituted and followed up after 1 year with promising results. This emphasizes the importance of neonatal screening for lipid vacuolations in peripheral blood in all cases of congenital ichthyosis.

  6. The storage capacity of cocoa seeds (Theobroma cacao L.) through giving Polyethylene Glycol (PEG) in the various of storage container

    Science.gov (United States)

    Lahay, R. R.; Misrun, S.; Sipayung, R.

    2018-02-01

    Cocoa is plant which it’s seed character is recalcitrant. Giving PEG and using various of storage containers was hoped to increase storage capacity of cocoa seeds as long as period of saving. The reseach was aimed to identify the storage capacity of cocoa seeds through giving PEG in the various of storage containers. Research took place in Hataram Jawa II, Kabupaten Simalungun, Propinsi Sumatera Utara, Indonesia. The method of this research is spit-split plot design with 3 replication. Storage period was put on main plot which was consisted of 4 level, PEG concentration was put on sub plot, consisted of 4 level and storage container was put on the sub sub plot consisted of 3 types. The results showed that until 4 days at storage with 45 % PEG concentration at all storage container, percentage of seed germination at storage can be decreased to be 2.90 %, and can be defensed until 16 days with 45 % PEG concentration at perforated plastic storage container. Percentage of molded seeds and seed moisture content were increased with added period of storage but seed moisture content was increased until 12 days at storage and was decreased at 16 days in storage.

  7. LRRK2 mediated Rab8a phosphorylation promotes lipid storage.

    Science.gov (United States)

    Yu, Miao; Arshad, Muhammad; Wang, Wenmin; Zhao, Dongyu; Xu, Li; Zhou, Linkang

    2018-02-27

    Several mutations in leucine rich repeat kinase 2 (LRRK2) gene have been associated with pathogenesis of Parkinson's disease (PD), a neurodegenerative disorder marked by resting tremors, and rigidity, leading to Postural instability. It has been revealed that mutations that lead to an increase of kinase activity of LRRK2 protein are significantly associated with PD pathogenesis. Recent studies have shown that some Rab GTPases, especially Rab8, serve as substrates of LRRK2 and undergo phosphorylation in its switch II domain upon interaction. Current study was performed in order to find out the effects of the phosphorylation of Rab8 and its mutants on lipid metabolism and lipid droplets growth. The phosphorylation status of Rab8a was checked by phos-tag gel. Point mutant construct were generated to investigate the function of Rab8a. 3T3L1 cells were transfected with indicated plasmids and the lipid droplets were stained with Bodipy. Fluorescent microscopy experiments were performed to examine the sizes of lipid droplets. The interactions between Rab8a and Optineurin were determined by immunoprecipitation and western blot. Our assays demonstrated that Rab8a was phosphorylated by mutated LRRK2 that exhibits high kinase activity. Phosphorylation of Rab8a on amino acid residue T72 promoted the formation of large lipid droplets. T72D mutant of Rab8a had higher activity to promote the formation of large lipid droplets compared with wild type Rab8a, with increase in average diameter of lipid droplets from 2.10 μm to 2.46 μm. Moreover, phosphorylation of Rab8a weakened the interaction with its effector Optineurin. Y1699C mutated LRRK2 was able to phosphorylate Rab8a and phosphorylation of Rab8a on site 72 plays important role in the fusion and enlargement of lipid droplets. Taken together, our study suggests an indirect relationship between enhanced lipid storage capacity and PD pathogenesis.

  8. Effect of polyethylene glycol 6000 and storage period on seed quality of cocoa (Theobroma cacao L.

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    Astiti Rahayu

    2014-05-01

    Full Text Available Increased productivity of cocoa needs high quality of cocoa seeds which are generally provided by certified seed gardens located far from smallholders farm, where seed delivery takes long time and may reduce physiological quality of seeds. One effort to maintain the seed quality is by treatment the seeds with polyethylene glycol6000 (PEG 6000. This study was aimed to study the interaction of PEG 6000 concentration and storage period on cocoa seed quality, and to determine the best concentration of PEG 6000 and storage period to maintain cocoa seed quality. The research was conducted in seed storage room, seed laboratory and green houseof PPPPTK, Cianjur. The experimental design used was a factorial completely randomized design and each combination treatment repeated three times. The first factor was concentration of PEG 6000 of 0%, 20%, 40%, and 60%, and the second factor was the storage period of 3 weeks, 6 weeks, 9 weeks, and 12 weeks. The results showed that concentration of PEG 60% with three weeks storage period was able to minimize the number of moldy seeds. The concentration of PEG 20%, 40%, and 60% were able to prevent seed germination in storage until six weeks. Concentration of PEG 6000 20% was able to maintain moisture content, seed germination, and germination rate.Key words:cocoa seed, storage, recalcitrant, PEG

  9. Quality characteristics of irradiated sesame seeds during storage

    International Nuclear Information System (INIS)

    Swailam, H.M.

    2009-01-01

    The effect of using gamma radiation 60 Co on the microbial load , presence of some pathogens, viscosity, oil, fatty acids and amino acids properties of sesame seeds were investigated.The condition storage at ambient temperature (20-28 deg c) was held for one year. Pre-packaged sesame seeds were found to be heavily contaminated with bacteria and fungi. The total bacterial counts were 1,9x10 4 cfu/g,whereas total fungal counts were 3.4x10 4 cfu/g.Samples showed low number of the pathogenic bacterial in control . It was found that the microbiological population greatly reduced with a dose of 10 kGy without affecting their quality attributes , irradiation doses used linearly reduced the viscosity of sesame seeds samples. These irradiation doses caused a decrease in the content of some fatty acids and amino acids and increase in others. At the end of storage period, it is clear that , irradiation dose of 10 kGy greatly reduced the counts of total bacterial count, total fungal count and spore former bacterial to less than 10 cfu/ g. The average counts of faecal coliforms , staphylococcus aureus and enterococcus faecalis did not detected in samples receiving 5 and 10 kGy . the flow index (n) and consistency index (k) were decreased after 12 month of storage in irradiated sesame with 10 kGy. Also, there was a slight increase in acid and peroxide value of oil extracted from sesame seeds treatment with 10 kGy. Meanwhile, there was a decrease in iodine, saponification value and very little decrease in the refractive index. It was found that slightly decreased in total unsaturated fatty acid (0.12%) and total amino acid (0.63%)in sesame irradiated at 10 kGy . so, it can be concluded that 10 kGy of γ-radiation suffice the purpose of this work

  10. Proteomic analysis of the seed development in Jatropha curcas: from carbon flux to the lipid accumulation.

    Science.gov (United States)

    Liu, Hui; Wang, Cuiping; Komatsu, Setsuko; He, Mingxia; Liu, Gongshe; Shen, Shihua

    2013-10-08

    To characterize the metabolic signatures of lipid accumulation in Jatropha curcas seeds, comparative proteomic technique was employed to profile protein changes during the seed development. Temporal changes in comparative proteome were examined using gels-based proteomic technique at six developmental stages for lipid accumulation. And 104 differentially expressed proteins were identified by MALDI-TOF/TOF tandem mass spectrometry. These protein species were classified into 10 functional categories, and the results demonstrated that protein species related to energy and metabolism were notably accumulated and involved in the carbon flux to lipid accumulation that occurs primarily from early to late stage in seed development. Glycolysis and oxidative pentose phosphate pathways were the major pathways of producing carbon flux, and the glucose-6-phosphate and triose-phosphate are the major carbon source for fatty acid synthesis. Lipid analysis revealed that fatty acid accumulation initiated 25days after flowering at the late stage of seed development of J. curcas. Furthermore, C16:0 was initially synthesized as the precursor for the elongation to C18:1 and C18:2 in the developing seeds of J. curcas. Together, the metabolic signatures on protein changes in seed development provide profound knowledge and perspective insights into understanding lipid network in J. curcas. Due to the abundant oil content in seeds, Jatropha curcas seeds are being considered as the ideal materials for biodiesel. Although several studies had carried out the transcriptomic project to study the genes expression profiles in seed development of J. curcas, these ESTs hadn't been confirmed by qRT-PCR. Yet, the seed development of J. curcas had been described for a pool of developing seeds instead of being characterized systematically. Moreover, cellular metabolic events are also controlled by protein-protein interactions, posttranslational protein modifications, and enzymatic activities which

  11. The Effect of Storage on Some Properties of 3 Different Ground Poppy Seed Fats

    OpenAIRE

    Maden, Besim; Yalçın, Seda

    2017-01-01

    Thepoppy seed is a traditional product in Afyon. There are there type poppy seed. Theseare white poppy seed, yellow poppy seed and gray poppy seed. The objective of thisstudy is to investigate the effect of storage at 15-20⁰C for several times (0, 7,15, 30, 45 and 60 days) on some properties of 3 different poppy seed fats. In thisstudy, fats were obtained from white poppy seed (TMO1), yellow poppy seed (TMO2)and gray poppy seed (Afyon-95). Then free fatty acid amount (%), peroxide value(meq O...

  12. Seed storage-mediated dormancy alleviation in Fabaceae from campo rupestre

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    Naïla Nativel

    2015-09-01

    Full Text Available ABSTRACTWe studied the effects of seed storage on germination and dormancy alleviation in three species of Fabaceae endemic to campo rupestrein southeastern Brazil. Fresh seeds of Collaea cipoensis, Mimosa maguirei and Mimosa foliolosawere set to germinate and germination of seeds after four, five and 13 years of storage was tested. Seed viability was maintained for all species after the full storage period. Seed storage significantly increased germination percentage and decreased germination time for C. cipoensisand M. foliolosa, suggesting the alleviation of physical dormancy with storage. However, we did not find evidence of dormancy alleviation in M. maguirei since stored seeds showed a decrease in germination in comparison to that of fresh seeds. Our data indicate species-specific storage-mediated dormancy alleviation, which will have important implications for restoration of campo rupestre.

  13. Variation of lipid and fatty acid compositions in Thai Perilla seeds grown at different locations

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    Maitree Suttajit

    2006-03-01

    Full Text Available Perilla or Nga-Kee-Mon (Perilla frutescens seed has long been known as a rich source of α-linolenic acid (18:3, n-3. It is widely cultivated throughout Thailand. However, there are no data on the variation of lipid and fatty acid compositions among crops from different regions. The aim of this study was to examine the compositions of lipids and fatty acids in Thai perilla seed grown at different locations. Two different perilla seeds were harvested from Maehongsorn and Chiang Mai districts, and one commercial perilla was purchased from local market. Seeds were ground, lipid was extracted with chloroform: methanol (2:1, v/v and its composition determined by Iatroscan (TLC/FID. Fatty acid composition was analyzed with GLC using standard methods. Lipid content was between 34-36% (w/w. Triacylglycerol was a predominant lipid in perilla seed (97% of total lipids, and a minor component was phytosterol (3% of total lipids. The ratio of saturates: monounsaturates: polyunsaturates was approximately 1: 1: 8. Most predominant fatty acid was α-linolenic acid (18:3, n-3 (55-60% of total fatty acid. Seeds from Maehongsorn district had the highest concentration of α-linolenic acid, and commercial perilla had the lowest (P<0.05. Other two predominant fatty acids were linoleic acid (18:2, n-6 (18-22% of total fatty acid and oleic acid (18:1 (11-13% of total fatty acid. The results showed that the compositions of lipids and fatty acids in Thai perilla seeds varied significantly among samples from different locations.

  14. Dorfman-Chanarin syndrome: A rare neutral lipid storage disease

    Directory of Open Access Journals (Sweden)

    Mitra Souvik

    2010-10-01

    Full Text Available Dorfman-Chanarin syndrome is a rare neutral lipid storage disorder characterized by ichthyosis, lipid vacuolations in peripheral leucocytes, and multisystem involvement. It is an autosomal recessive disorder caused by mutations in the CGI-58 gene. A total of 42 cases have been reported worldwide till February 2009 out of which 4 have been previously reported from India. We report a case of a 20-month-old male with congenital ichthyosis, organomegaly, and bilateral cryptorchidism. Examination of the peripheral smear revealed lipid vacuoles in the leucocytes consistent with Jordan′s anomaly, which was confirmed by transmission electron microscopy. Liver biopsy revealed micronodular cirrhosis with macrovesicular steatosis while skin biopsy showed ichthyosis vulgaris. Dorfman-Chanarin syndrome was diagnosed on the basis of clinical and laboratory criteria with certain unreported manifestations. Dietary modifications were instituted and followed up after 1 year with promising results. This emphasizes the importance of neonatal screening for lipid vacuolations in peripheral blood in all cases of congenital ichthyosis.

  15. CONSERVATION OF THE VIABILITY AND VIGOR OF Araucaria angustifolia (Bert. O. Kuntze SEEDS DURING THE STORAGE

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    Cristhyane Garcia

    2014-12-01

    Full Text Available The conservation of Araucaria seeds is widely compromised in function of their recalcitrant feature, which hampers the planning of recovery actions of the degraded populations. Therefore, the objective of this study was to monitor the physiological changes in Araucaria seeds under controlled storage conditions, in order to get insights as to the viability and vigor conservation. The physiological quality of freshly harvested seeds was evaluated and every 60 days throughout the 180 days-storage period in laboratory ambient without thermal control, refrigerator (5 ° C, and freezer (-18 ° C until the final period of 180 days. After each sampling period, the seed viability (germination and tetrazolium tests and vigor (artificial aging, germination speed index – IVG and electrical conductivity were assessed. A reduction in the normal seedlings percentage was noticed over the period of storage of Araucaria seeds. The conservation in freezer and the lack of thermal control caused a complete loss of the seed viability at 60 and 180 days of storage, respectively. However, the refrigerator storage promoted the conservation of seed viability, with 64% germination after 180 days of storage, an event associated with the reduction of the metabolic activity of seeds. Based on the viability and vigor tests, it was concluded that storage in refrigerator provided longer storage periods to Araucaria seeds in comparison to the other storage conditions herein studied.

  16. Cerebrotendinous xanthomatosis (a rare lipid storage disorder): a case report.

    Science.gov (United States)

    Razi, Syed Mohd; Gupta, Abhinav Kumar; Gupta, Deepak Chand; Gutch, Manish; Gupta, Keshav Kumar; Usman, Syeda Iqra

    2016-04-19

    Cerebrotendinous xanthomatosis is a very rare autosomal recessive lipid storage disorder affecting bile acid biosynthesis. It is manifested by subtle neurological and non-neurological symptoms due to abnormal tissue lipid deposition. Diagnosis is usually delayed but early diagnosis and replacement therapy can prevent devastating neurological sequelae. We present a case of a 25-year-old Asian Indian woman who presented with gait difficulty, fusiform swellings of bilateral tendo-Achilles and infrapatellar tendons, along with history of bilateral cataract surgery 1 year earlier. The diagnosis was made on the basis of clinical, biochemical, imaging, and histopathological analysis and replacement therapy was started. The peculiarity of the present case is the absence of any neurological manifestations which are usually the early clues to the diagnosis of cerebrotendinous xanthomatosis. The present case report emphasizes the fact that early age bilateral cataracts along with bilateral tendo-Achilles xanthomas can be early pointers toward the diagnosis of cerebrotendinous xanthomatosis.

  17. Physiological behavior of bean's seeds and grains during storage

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    FLÁVIA D.R. CASSOL

    2016-06-01

    Full Text Available Beans are one of the most used foods to meet the energy needs of the Brazilian diet, requiring farmers to use high seed physiological potential. The aim was to evaluate the physiological quality of beans stored for 360 days. Analyses were performed at 0, 30, 90, 180, 270, and 360 days after receiving the seeds (S1 and S2 and grains (G1 and G2 of BRS Splendor. Tests of germination, accelerated aging, cold, speed of germination, average length of shoots, and root were performed. The experimental design was completely randomized split-plot in time and the means were compared through Tukey test at 5% probability. Seed germination was not affected in S2, while the drop in S1 and G1 was significant. The vigor of grains from field 1 declined from 91 to 50% and from 93% to 76% by accelerated aging and cold, respectively, after 360 days. The germination speed tests performed showed a decreased during the experiment. The grains from field 1 had lower physiological quality. The accelerated aging and cold tests, through the speed of germination parameter, showed decrease in the vigor of the Splendor BRS. The storage period influenced the physiological quality of the beans tested.

  18. Physiological behavior of bean's seeds and grains during storage.

    Science.gov (United States)

    Cassol, Flávia D R; Fortes, Andréa M T; Mendonça, Lorena C; Buturi, Camila V; Marcon, Thaís R

    2016-05-31

    Beans are one of the most used foods to meet the energy needs of the Brazilian diet, requiring farmers to use high seed physiological potential. The aim was to evaluate the physiological quality of beans stored for 360 days. Analyses were performed at 0, 30, 90, 180, 270, and 360 days after receiving the seeds (S1 and S2) and grains (G1 and G2) of BRS Splendor. Tests of germination, accelerated aging, cold, speed of germination, average length of shoots, and root were performed. The experimental design was completely randomized split-plot in time and the means were compared through Tukey test at 5% probability. Seed germination was not affected in S2, while the drop in S1 and G1 was significant. The vigor of grains from field 1 declined from 91 to 50% and from 93% to 76% by accelerated aging and cold, respectively, after 360 days. The germination speed tests performed showed a decreased during the experiment. The grains from field 1 had lower physiological quality. The accelerated aging and cold tests, through the speed of germination parameter, showed decrease in the vigor of the Splendor BRS. The storage period influenced the physiological quality of the beans tested.

  19. Rapid biodiesel synthesis from waste pepper seeds without lipid isolation step.

    Science.gov (United States)

    Lee, Jechan; Kim, Jieun; Ok, Yong Sik; Kwon, Eilhann E

    2017-09-01

    In situ transformation of lipid in waste pepper seeds into biodiesel (i.e., fatty acid methyl esters: FAMEs) via thermally-induced transmethylation on silica was mainly investigated in this study. This study reported that waste pepper seeds contained 26.9wt% of lipid and that 94.1% of the total lipid in waste pepper seeds could be converted into biodiesel without lipid extraction step for only ∼1min reaction time. This study also suggested that the optimal temperature for in situ transmethylation was identified as 390°C. Moreover, comparison of in situ process via the conventional transmethylation catalyzed by H 2 SO 4 showed that the introduced biodiesel conversion in this study had a higher tolerance against impurities, thereby being technically feasible. The in situ biodiesel production from other oil-bearing food wastes can be studied. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Minor lipid components of some Acacia species: potential dietary health benefits of the unexploited seeds

    Directory of Open Access Journals (Sweden)

    Nasri Nizar

    2012-05-01

    Full Text Available Abstract Background Oilseed samples from four Acacia species ( A. cyclops, A. ligulata, A. salicina and A. cyanophylla were analyzed in order to evaluate the potential nutritional value of their unexploited seeds. Methods Samples were collected from different Tunisian geographic locations. Seed oils were extracted and carotenoids, tocopherols and sterols were analyzed using chromatographic methods. Results The studied Acacia seeds seem to be quite rich in lipids (from 6% to 12%. All Acacia species contain mainly the xanthophylls zeaxanthin and lutein compounds: from ca. 38 mg.kg-1 of total lipids (A. cyclops to ca. 113 mg.kg-1 of total lipids (A. cyanophylla. Total tocopherols varied from ca. 221 mg.kg-1 of total lipids (A. cyclops to ca. 808 mg.kg-1 of total lipids (A. ligulata. Sterols are highly present and their contents ranged between ca. 7 g. kg-1 of total lipids (A. salicina and 11 g. kg-1 of total lipids (A. cyclops. Conclusion This study highlights that these unexploited seeds might have a potential nutritional value and encourages researchers to more explore and find developments for these plants for healthy purposes.

  1. PHYSIOLOGICAL QUALITY OF Araucaria angustifolia (Bertol. Kuntze SEEDS SUBMITTED TO DIFFERENT STORAGE CONDITIONS AND ESCARIFICATION

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    Álvaro Valente Caçola

    2010-08-01

    Full Text Available The seeds of Araucaria angustifolia (Bertol. Kuntze are recalcitrants and, therefore, they lose rapidly the viability after the harvest, limiting their use for nursery production of seedlings. This work was carried out to investigate the effects of cold storage duration, conservation method, and escarification on germination and vigour of seeds of Araucaria angustifolia. The seeds were cold stored (0-1oC/90-95% RH for 0, 60, 120, and 180 days, under normal air condictions (NA, modified atmosphere (MA, and controlled atmosphere (CA storage. For each cold storage duration, after removal from cold storage, the seeds were submitted or not to scarification (by cutting a small portion of the apical seed tegument, avoiding the damage of the endosperm, letting the germinate in a chamber with controlled environment, in plastic trays filled with vermiculite, for 60 days. The experiment followed the completely randomized factorial design (4x3x2, with four cold storage durations (0, 60, 120, and 180 days, three storage methods (NA, MA, and CA, with or without escarification, and four replicates. There was a good preservation of physiological quality for seeds submitted to different conservation methods in cold storage along the 180-day period. However, there was a faster germination and initial growth of seedlings for seeds left in cold storage for 60 days than in seeds assessed at harvest or left in cold storage for 120-180 days. The NA storage of seeds in perfurated plastic bags was as efficient as MA and CA storage to preserve seeds quality. Seeds scarification increased vigor and promoted seedlings initial growth of Araucaria angustifolia after being removed from cold storage.

  2. Storage sites in seeds of Caesalpinia echinata and C. ferrea (Leguminosae with considerations on nutrients flow

    Directory of Open Access Journals (Sweden)

    Simone de Pádua Teixeira

    2008-02-01

    Full Text Available The seeds of Caesalpinia echinata and C. ferrea behaved as typical endospermic seeds, despite their different morphological classification (exendospermic seeds were described for C. echinata and endospermic seeds for C. ferrea. Then, the aim of this work was to compare, under ultrastructural and histochemical terms, the nature of the storage substances and their accumulation sites, as well as the nutrient flow in seeds of these species. Cotyledons in C. echinata accumulate carbohydrates, lipids and proteins, which are mobilized from the outer to the inner parts as revealed by the position of plasmodesmata. Endosperm in C. ferrea accumulates carbohydrates and in C. echinata accumulates substances during the initial embryogenic phases. Such tissue develops a chalazal haustorium that is responsible for the transport of substances into the endosperm itself and from it into the embryo, confirmed by the presence of transference cells.As sementes de Caesalpinia echinata e C. ferrea comportam-se como endospérmicas, apesar de descritas na literatura como exendospérmicas e endospérmicas, respectivamente. Desta forma, o objetivo deste trabalho foi comparar, em termos ultra-estrutural e histoquímico, a natureza das substâncias de reserva e seus tecidos acumuladores, bem como o fluxo de nutrientes nas sementes destas espécies. Os cotilédones em C. echinata acumulam carboidratos, lipídios e proteínas, mobilizados da periferia para o centro, como visto pelo posicionamento dos plasmodesmas. O endosperma em C. ferrea acumula carboidratos e lipídios, e em C. echinata, acumula substâncias nos estádios iniciais da embriogênese. Este tecido desenvolve um haustório calazal agressivo, que transporta substâncias para o endosperma propriamente dito e deste para o embrião, fato confirmado pela presença de células de transferência no endosperma.

  3. Triacylglycerol Storage in Lipid Droplets in Procyclic Trypanosoma brucei.

    Directory of Open Access Journals (Sweden)

    Stefan Allmann

    Full Text Available Carbon storage is likely to enable adaptation of trypanosomes to nutritional challenges or bottlenecks during their stage development and migration in the tsetse. Lipid droplets are candidates for this function. This report shows that feeding of T. brucei with oleate results in a 4-5 fold increase in the number of lipid droplets, as quantified by confocal fluorescence microscopy and by flow cytometry of BODIPY 493/503-stained cells. The triacylglycerol (TAG content also increased 4-5 fold, and labeled oleate is incorporated into TAG. Fatty acid carbon can thus be stored as TAG in lipid droplets under physiological growth conditions in procyclic T. brucei. β-oxidation has been suggested as a possible catabolic pathway for lipids in T. brucei. A single candidate gene, TFEα1 with coding capacity for a subunit of the trifunctional enzyme complex was identified. TFEα1 is expressed in procyclic T. brucei and present in glycosomal proteomes, Unexpectedly, a TFEα1 gene knock-out mutant still expressed wild-type levels of previously reported NADP-dependent 3-hydroxyacyl-CoA dehydrogenase activity, and therefore, another gene encodes this enzymatic activity. Homozygous Δtfeα1/Δtfeα1 null mutant cells show a normal growth rate and an unchanged glycosomal proteome in procyclic T. brucei. The decay kinetics of accumulated lipid droplets upon oleate withdrawal can be fully accounted for by the dilution effect of cell division in wild-type and Δtfeα1/Δtfeα1 cells. The absence of net catabolism of stored TAG in procyclic T. brucei, even under strictly glucose-free conditions, does not formally exclude a flux through TAG, in which biosynthesis equals catabolism. Also, the possibility remains that TAG catabolism is completely repressed by other carbon sources in culture media or developmentally activated in post-procyclic stages in the tsetse.

  4. Muscle MRI in neutral lipid storage disease (NLSD).

    Science.gov (United States)

    Garibaldi, Matteo; Tasca, Giorgio; Diaz-Manera, Jordi; Ottaviani, Pierfancesco; Laschena, Francesco; Pantoli, Donatella; Gerevini, Simonetta; Fiorillo, Chiara; Maggi, Lorenzo; Tasca, Elisabetta; D'Amico, Adele; Musumeci, Olimpia; Toscano, Antonio; Bruno, Claudio; Massa, Roberto; Angelini, Corrado; Bertini, Enrico; Antonini, Giovanni; Pennisi, Elena Maria

    2017-07-01

    Neutral lipid storage disease (NLSD) is a rare inherited disorder of lipid metabolism resulting in lipid droplets accumulation in different tissues. Skeletal muscle could be affected in both two different form of disease: NLSD with myopathy (NLSD-M) and NLSD with ichthyosis (NLSD-I). We present the muscle imaging data of 12 patients from the Italian Network for NLSD: ten patients presenting NLSD-M and two patients with NLSD-I. In NLSD-M gluteus minimus, semimembranosus, soleus and gastrocnemius medialis in the lower limbs and infraspinatus in the upper limbs were the most affected muscles. Gracilis, sartorius, subscapularis, pectoralis, triceps brachii and sternocleidomastoid were spared. Muscle involvement was not homogenous and characteristic "patchy" replacement was observed in at least one muscle in all the patients. Half of the patients showed one or more STIR positive muscles. In both NLSD-I cases muscle involvement was not observed by T1-TSE sequences, but one of them showed positive STIR images in more than one muscle in the leg. Our data provides evidence that muscle imaging can identify characteristic alterations in NLSD-M, characterized by a specific pattern of muscle involvement with "patchy" areas of fatty replacement. Larger cohorts are needed to assess if a distinct pattern of muscle involvement exists also for NLSD-I.

  5. Effects of gamma irradiation on chickpea seeds vis-a-vis total seed storage proteins, antioxidant activity and protein profiling.

    Science.gov (United States)

    Bhagyawant, S S; Gupta, N; Shrivastava, N

    2015-10-23

    The present work describes radiation—induced effects on seed composition vis—à—vis total seed proteins, antioxidant levels and protein profiling employing two dimensional gel electrophoresis (2D—GE) in kabuli and desi chickpea varities. Seeds were exposed to the radiation doses of 1,2,3,4 and 5 kGy. The total protein concentrations decreased and antioxidant levels were increased with increasing dose compared to control seed samples. Radiation induced effects were dose dependent to these seed parameters while it showed tolerance to 1 kGy dose. Increase in the dose was complimented with increase in antioxidant levels, like 5 kGy enhanced % scavenging activities in all the seed extracts. Precisely, the investigations reflected that the dose range from 2 to 5 kGy was effective for total seed storage proteins, as depicted quantitatively and qualitative 2D—GE means enhance antioxidant activities in vitro.

  6. JCL Roundtable: enzyme replacement therapy for lipid storage disorders.

    Science.gov (United States)

    Brown, W Virgil; Desnick, Robert J; Grabowski, Gregory A

    2014-01-01

    There are several inherited disorders that involve abnormal storage of lipids in tissues leading to severe compromise of organs. Sadly, these are often accompanied by lifelong morbidity and early mortality. Disorders such as Gaucher, Fabry, and lysosomal acid lipase deficiencies (Wolman and cholesteryl ester storage diseases) have been known for many years, and provide a difficult and frustrating set of problems for patients, their families, and their physicians. With recombinant methods of protein synthesis, it is now possible to literally replace the defective enzymes that underlie the basic pathophysiology of many such disorders. The delivery of these enzymes into the affected cells is possible because of their location in the lysosomes where the natural degradation of their lipid substrates occurs. I have asked 2 well-known investigators to join us for this Roundtable. These are professors who have been involved with the research that has made this type of therapy possible and who have participated in the clinical trials that demonstrated the value of enzyme replacement therapy. They are Dr. Robert Desnick, dean of Genetic and Genomic Medicine and professor and chairman emeritus of the Department of Genetics and Genomic Sciences at the Icahn School of Medicine at Mount Sinai in New York City, and Dr. Gregory Grabowski, professor of Microbiology, Biochemistry, and Pediatrics, at the University of Cincinnati College of Medicine. Dr. Grabowski recently retired from that school to become the chief science officer of Synageva, a company involved in producing enzymes for this type of therapy. Copyright © 2014 National Lipid Association. Published by Elsevier Inc. All rights reserved.

  7. Tolerance of Ruppia sinensis Seeds to Desiccation, Low Temperature, and High Salinity With Special Reference to Long-Term Seed Storage.

    Science.gov (United States)

    Gu, Ruiting; Zhou, Yi; Song, Xiaoyue; Xu, Shaochun; Zhang, Xiaomei; Lin, Haiying; Xu, Shuai; Yue, Shidong; Zhu, Shuyu

    2018-01-01

    Seeds are important materials for the restoration of globally-threatened marine angiosperm (seagrass) populations. In this study, we investigated the differences between different Ruppia sinensis seed types and developed two feasible long-term R. sinensis seed storage methods. The ability of R. sinensis seeds to tolerate the short-term desiccation and extreme cold had been investigated. The tolerance of R. sinensis seeds to long-term exposure of high salinity, cold temperature, and desiccation had been considered as potential methods for long-term seed storage. Also, three morphological and nine physiological indices were measured and compared between two types of seeds: Shape L and Shape S. We found that: (1) wet storage at a salinity of 30-40 psu and 0°C were the optimal long-term storage conditions, and the proportion of viable seeds reached over 90% after a storage period of 11 months since the seeds were collected from the reproductive shoots; (2) dry condition was not the optimal choice for long-term storage of R. sinensis seeds; however, storing seeds in a dry condition at 5°C and 33 ± 10% relative humidity for 9 months had a relatively high percentage (74.44 ± 2.22%) of viable seeds, consequently desiccation exposure could also be an acceptable seed storage method; (3) R. sinensis seeds would lose vigor in the interaction of extreme cold (-27°C) and desiccation; (4) there were significant differences in seed weight, seed curvature, and endocarp thickness between the two types of seeds. These findings provided fundamental physiological information for R. sinensis seeds and supported the long-term storage of its seeds. Our results may also serve as useful reference for seed storage of other threatened seagrass species and facilitate their ex situ conservation and habitat restoration.

  8. Tolerance of Ruppia sinensis Seeds to Desiccation, Low Temperature, and High Salinity With Special Reference to Long-Term Seed Storage

    Directory of Open Access Journals (Sweden)

    Ruiting Gu

    2018-03-01

    Full Text Available Seeds are important materials for the restoration of globally-threatened marine angiosperm (seagrass populations. In this study, we investigated the differences between different Ruppia sinensis seed types and developed two feasible long-term R. sinensis seed storage methods. The ability of R. sinensis seeds to tolerate the short-term desiccation and extreme cold had been investigated. The tolerance of R. sinensis seeds to long-term exposure of high salinity, cold temperature, and desiccation had been considered as potential methods for long-term seed storage. Also, three morphological and nine physiological indices were measured and compared between two types of seeds: Shape L and Shape S. We found that: (1 wet storage at a salinity of 30–40 psu and 0°C were the optimal long-term storage conditions, and the proportion of viable seeds reached over 90% after a storage period of 11 months since the seeds were collected from the reproductive shoots; (2 dry condition was not the optimal choice for long-term storage of R. sinensis seeds; however, storing seeds in a dry condition at 5°C and 33 ± 10% relative humidity for 9 months had a relatively high percentage (74.44 ± 2.22% of viable seeds, consequently desiccation exposure could also be an acceptable seed storage method; (3 R. sinensis seeds would lose vigor in the interaction of extreme cold (-27°C and desiccation; (4 there were significant differences in seed weight, seed curvature, and endocarp thickness between the two types of seeds. These findings provided fundamental physiological information for R. sinensis seeds and supported the long-term storage of its seeds. Our results may also serve as useful reference for seed storage of other threatened seagrass species and facilitate their ex situ conservation and habitat restoration.

  9. Effect of baking and storage on the fatty acid composition of cookies with chia seed meal

    Science.gov (United States)

    Chia (Salvia hispanica L.) seed is an ancient crop of the Aztecs that has recently gained interest as a functional food. Chia seeds are a good source of polyphenolic compounds with antioxidant activity. However, the effect of baking and storage on the antioxidant properties of chia seed meal is not ...

  10. The Effects of Storage on Germination Characteristics and Enzyme Activity of Sorghum Seeds

    Directory of Open Access Journals (Sweden)

    Azadi M.S.

    2013-11-01

    Full Text Available Seed moisture content (MC and storage temperature are the most important factors affecting seed longevity and vigor. Exposure to warm, moist air is principally responsible for this. Proper storage and optimum seed moisture content can affect the grain quality significantly. The purpose of this study was to evaluate the different storage treatments on seed quality of sorghum. The seed materials were fresh without any storage period. For storage treatments, 3 seed moisture contents (6, 10, 14 % were stored for 8 month in 0.5 L capacity sealed aluminum foil packet in 0.3 bar inside incubators set at 4 temperatures (5, 15, 25, 35 °C. After storage time, the higher the storage temperature, the lower was the grain quality of sorghum. The highest germination percentage, germination index, normal seedling percentage were achieved in control conditions (0 day of storage. Our results showed that increasing storage duration resulted higher reduction in germination characteristics. Also our results showed that, germination percentage, means time to germination, germination index, normal seedling percentage decrease significantly by storage. Enzyme activity decrease significantly by increased in storage.

  11. [STUDY OF LIPIDS SEED'S OIL OF VITEX AGNUS CASTUS GROWING IN GEORGIA].

    Science.gov (United States)

    Kikalishvili, B; Zurabashvili, D; Sulakvelidze, Ts; Malania, M; Turabelidze, D

    2016-07-01

    There was established the lipid composition of the seeds of Vitex agnus castus L. by the qualitative and quantitative methods of analyses. There were received neutral lipids from the seeds by extraction with hexane in the yield 10%, counted on dry material. For the divide of neutral lipids there was used silica gel plates LS 5/40 in the systems of solvents: 1. petroleum ether-diethylether-acidum aceticum (85:14:1), 2. hexane-diethylether (1:1). After obtaining neutral lipids from the residual plant shrot pollar lipids was extracted with the mixture of chloroform-methanol (2:1) and was divided on silica gel plates LS 5/40, mobile phase: 1. chloroform-methanol-25% ammonium hydrate 2. chloroform-methanol icy acetic acid-water (170:25:25:6). In the sum of polar lipids qualitatively were established phospholipids: lisophosphatidylcholine, phosphatidylinosit, phospatidylethanolamine and N-acylphosphatidylethanolamine, in neutral lipids, hydrocarbons, triglycerids, free fatty acids and sterines. By the method of high performance liquid chromatography analyses there were identified following free fatty acids: lauric, myristic, palmitic, stearic, linolic, linolenic, arachidic and begenic, unsaturated oleic and polyunsaturated linolic and linolenic acids. obtained oil with unique composition from the seeds of Vitex agnus-castus indicates to its high biological activity and importance for usage in medicine.

  12. Characterization of seed storage protein patterns of Heliotropium digynum

    Directory of Open Access Journals (Sweden)

    Mona Soliman Alwhibi

    2017-09-01

    Full Text Available Heliotropium digynum, is a shrub that has ecological importance. The height of the plant differs from one population to another and the difference in length of the inflorescence can be attributed to environmental factors, such as rainfall or type of soil and temperature. To date, no study has shed light on estimation in seed samples of H. digynum in Saudi Arabia. So, the aim is to evaluate and characterize the protein patterns of seed storage proteins of H. digynum to be used as fingerprint of this plant in Saudi Arabia. It is collected from different locations in the central region of Saudi Arabia and total protein extraction from plant was compared in SDS-PAGE. The genetic relationships among all cultivars were analyzed using UPGMA and NJ using Total Lab TL and in the same way using Jaccard Similarity Coefficient dendrogram using STATISTICA (ver.8 software. Results, our data show that amounts of protein are different, although they are of the same type or from the same geographical region. Amounts ranged between 22 and 1.5 mg/g of dry weight. Less amount of protein was obtained from the group of samples collected from Dir’iyah area, and the highest amount of protein was from the group of samples collected from Dyrab area in general.

  13. Characterization of seed storage protein patterns of Heliotropium digynum.

    Science.gov (United States)

    Alwhibi, Mona Soliman

    2017-09-01

    Heliotropium digynum , is a shrub that has ecological importance. The height of the plant differs from one population to another and the difference in length of the inflorescence can be attributed to environmental factors, such as rainfall or type of soil and temperature. To date, no study has shed light on estimation in seed samples of H. digynum in Saudi Arabia. So, the aim is to evaluate and characterize the protein patterns of seed storage proteins of H. digynum to be used as fingerprint of this plant in Saudi Arabia. It is collected from different locations in the central region of Saudi Arabia and total protein extraction from plant was compared in SDS-PAGE. The genetic relationships among all cultivars were analyzed using UPGMA and NJ using Total Lab TL and in the same way using Jaccard Similarity Coefficient dendrogram using STATISTICA (ver.8) software. Results, our data show that amounts of protein are different, although they are of the same type or from the same geographical region. Amounts ranged between 22 and 1.5 mg/g of dry weight. Less amount of protein was obtained from the group of samples collected from Dir'iyah area, and the highest amount of protein was from the group of samples collected from Dyrab area in general.

  14. EFFECTS OF NATURAL LONG STORAGE DURATION ON SEED ...

    African Journals Online (AJOL)

    lep

    2013-04-10

    Apr 10, 2013 ... germination and viability, seed vigor index and seed vigor under a 7.3% moisture content when .... containing two disks of Whatman filter papers soaked in distilled water. ..... desiccation tolerance (Review Update). Seed Sci.

  15. Quantitative trait loci associated with longevity of lettuce seeds under conventional and controlled deterioration storage conditions.

    Science.gov (United States)

    Schwember, Andrés R; Bradford, Kent J

    2010-10-01

    Lettuce (Lactuca sativa L.) seeds have poor shelf life and exhibit thermoinhibition (fail to germinate) above ∼25°C. Seed priming (controlled hydration followed by drying) alleviates thermoinhibition by increasing the maximum germination temperature, but reduces lettuce seed longevity. Controlled deterioration (CD) or accelerated ageing storage conditions (i.e. elevated temperature and relative humidity) are used to study seed longevity and to predict potential seed lifetimes under conventional storage conditions. Seeds produced in 2002 and 2006 of a recombinant inbred line (RIL) population derived from a cross between L. sativa cv. Salinas×L. serriola accession UC96US23 were utilized to identify quantitative trait loci (QTLs) associated with seed longevity under CD and conventional storage conditions. Multiple longevity-associated QTLs were identified under both conventional and CD storage conditions for control (non-primed) and primed seeds. However, seed longevity was poorly correlated between the two storage conditions, suggesting that deterioration processes under CD conditions are not predictive of ageing in conventional storage conditions. Additionally, the same QTLs were not identified when RIL populations were grown in different years, indicating that lettuce seed longevity is strongly affected by production environment. Nonetheless, a major QTL on chromosome 4 [Seed longevity 4.1 (Slg4.1)] was responsible for almost 23% of the phenotypic variation in viability of the conventionally stored control seeds of the 2006 RIL population, with improved longevity conferred by the Salinas allele. QTL analyses may enable identification of mechanisms responsible for the sensitivity of primed seeds to CD conditions and breeding for improved seed longevity.

  16. Induction of antioxidant enzyme activity and lipid peroxidation level in ion-beam-bombarded rice seeds

    Energy Technology Data Exchange (ETDEWEB)

    Semsang, Nuananong, E-mail: nsemsang@gmail.com [Molecular Biology Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200 (Thailand); Yu, LiangDeng [Plasma and Beam Physics Research Facility, Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200 (Thailand); Thailand Center of Excellence in Physics, Commission on Higher Education, 328 Si Ayutthaya Road, Bangkok 10400 (Thailand)

    2013-07-15

    Highlights: ► Ion beam bombarded rice seeds in vacuum. ► Studied seed survival from the ion bombardment. ► Determined various antioxidant enzyme activities and lipid peroxidation level. ► Discussed vacuum, ion species and ion energy effects. ► Attributed the changes to free radical formation due to ion bombardment. -- Abstract: Low-energy ion beam bombardment has been used to mutate a wide variety of plant species. To explore the indirect effects of low-energy ion beam on biological damage due to the free radical production in plant cells, the increase in antioxidant enzyme activities and lipid peroxidation level was investigated in ion-bombarded rice seeds. Local rice seeds were bombarded with nitrogen or argon ion beams at energies of 29–60 keV and ion fluences of 1 × 10{sup 16} ions cm{sup −2}. The activities of the antioxidant enzymes; superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione S-transferase (GST) and lipid peroxidation level were assayed in the germinated rice seeds after ion bombardment. The results showed most of the enzyme activities and lipid peroxidation levels in both the argon and nitrogen bombarded samples were higher than those in the natural control. N-ion bombardment could induce higher levels of antioxidant enzyme activities in the rice samples than the Ar-ion bombardment. Additional effects due to the vacuum condition were found to affect activities of some antioxidant enzymes and lipid peroxidation level. This study demonstrates that ion beam bombardment and vacuum condition could induce the antioxidant enzyme activity and lipid peroxidation level which might be due to free radical production in the bombarded rice seeds.

  17. Phisyological quality of Pinus elliotti Engelm. seeds subjected to differents storage methods

    Directory of Open Access Journals (Sweden)

    Adriano Geraldo Fonseca

    2012-09-01

    Full Text Available Due to the growing demand for forest products, it is necessary to increase knowledge about the tree species of economic importance, especially in relation to seed storage. One method to study forest species is the storage of seeds. Cryopreservation is the cheapest and the most efficient method of seed preservation. The objective of this study was to test the behavior of Pinus elliottii Engelm. seeds storaged on liquid nitrogen; laboratory environment and refrigerator. The experiment was conducted in the seed Laboratory of the Federal University of the Jequitinhonha and Mucuri Valley. The seeds were donated by Rigesa Ltda on June 2007. Three types of storage were tested: 1 liquid nitrogen temperature of -196 º C, 2 laboratory environment, 3 refrigerator. All treatments were evaluated at 0, 40, 80 and 120 days of storage. The vigor, germination and germination rate tests were mounted on gerbox with blotters and moistened with distilled water and kept in BOD incubator chamber at 25 º C. Seeds length, green mass and dry mass were also evaluated. The data were subjected to analysis of variance and means compared by Tukey test at 5% significance level. It was found that there was loss of quality of the Pinus elliottii seeds in all treatments. Cryopreservation of P. elliotii seeds can be used as an alternative of storage in genebanks.

  18. Induction of antioxidant enzyme activity and lipid peroxidation level in ion-beam-bombarded rice seeds

    Science.gov (United States)

    Semsang, Nuananong; Yu, LiangDeng

    2013-07-01

    Low-energy ion beam bombardment has been used to mutate a wide variety of plant species. To explore the indirect effects of low-energy ion beam on biological damage due to the free radical production in plant cells, the increase in antioxidant enzyme activities and lipid peroxidation level was investigated in ion-bombarded rice seeds. Local rice seeds were bombarded with nitrogen or argon ion beams at energies of 29-60 keV and ion fluences of 1 × 1016 ions cm-2. The activities of the antioxidant enzymes; superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione S-transferase (GST) and lipid peroxidation level were assayed in the germinated rice seeds after ion bombardment. The results showed most of the enzyme activities and lipid peroxidation levels in both the argon and nitrogen bombarded samples were higher than those in the natural control. N-ion bombardment could induce higher levels of antioxidant enzyme activities in the rice samples than the Ar-ion bombardment. Additional effects due to the vacuum condition were found to affect activities of some antioxidant enzymes and lipid peroxidation level. This study demonstrates that ion beam bombardment and vacuum condition could induce the antioxidant enzyme activity and lipid peroxidation level which might be due to free radical production in the bombarded rice seeds.

  19. Effect of dietary ethanol extracts of mango (Mangifera indica L.) on lipid oxidation and the color of chicken meat during frozen storage.

    Science.gov (United States)

    Freitas, Ednardo Rodrigues; da Silva Borges, Ângela; Pereira, Ana Lúcia Fernandes; Abreu, Virgínia Kelly Gonçalves; Trevisan, Maria Teresa Salles; Watanabe, Pedro Henrique

    2015-12-01

    The aim of this study was to evaluate the dietary effect of mango extracts on lipid stability and the coloring of broiler chicken breast meat during frozen storage. The treatments consisted of broiler chicken diet without antioxidants (control) and diets containing antioxidants: 200 ppm of butylated hydroxytoluene (BHT), 200 ppm of mango peel extract (MPE), 400 ppm of MPE, 200 ppm of mango seed extract (MSE), and 400 ppm of MSE. The broiler breasts were stored for 90 days and analysis of lipid oxidation and color was performed every 30 days. The thiobarbituric acid reactive substances values increased during storage and at 90 days, but the 400 ppm MSE treatment yielded lower values, indicating greater antioxidant activity. During storage, the lightness values decreased and the redness increased. Additions of 200 ppm BHT and 400 ppm MPE increased yellowness at 60 days of storage. Thus, mango peel and seed extracts added to broiler chicken diets reduce lipid oxidation and maintain color in breast meat during frozen storage, with mango seed extract at 400 ppm being the most effective. © 2015 Poultry Science Association Inc.

  20. Proximate content and lipid profile of seeds from Rapanea melanophloeos (The Cape Beech) tree

    International Nuclear Information System (INIS)

    Lotier, N.; Chivandi, E.; Lembede, B.W.; Ibrahim, K.G.; Nyakudya, T.T.

    2017-01-01

    Rapanea melanophloeos(Cape beech)is a fruit-bearing tree indigenous to Southern Africa which produces edible fruit. Seed samples obtained from ripe fruit of the Cape beech trees had their proximate analysis and lipid profiling done. The dry matter and ash contributed 91.29 +- 0.00 %, 1.50 +- 0.01 % of the mass of the seed. Whilst the other proximate analytes namely crude fibre, crude protein and ether extract made up 5.71 +- 0.43 %, 10.50 +- 0.49 % and 4.75 +- 0.09 percentage of the mass of the seeds respectively. The fatty acid profile of the seed oil revealed Linoleic acid (50.43 +- 0.38 %) to be the most dominant. R. melanophloeos seeds are not a viable source of nutrients. (author)

  1. Activity of water content and storage temperature on the seed-borne mycoflora of lens culinaris

    International Nuclear Information System (INIS)

    Rahim, S.; Dawar, S.

    2014-01-01

    Storage of seeds with high water content and temperatures favors the growth of mould fungi which in turn affect the germination of seeds while low temperature with low water content prevent the growth of storage fungi and help in maintaining seed viability for longer duration of time. Seed sample from Sukkur district was stored at 4 degree C and room temperature (25-30 degree C) with water content of 8, 13 and 17% for about 80 days. The fungi were isolated at 0, 20, 40, 60 and 80 days intervals. Highest infection percentage of fungi was observed at 13 and 17% water contents at room temperature after 20 days of storage. High infection percentage of storage fungi affected the germination of seeds. Aspergillus spp were the most dominant fungi. (author)

  2. Bioactive compounds in lipid fractions of pumpkin (Cucurbita sp) seeds for use in food.

    Science.gov (United States)

    Veronezi, Carolina Médici; Jorge, Neuza

    2012-06-01

    Seeds are considered to be agro-industrial residues, which can be used as source of macronutrients and/or raw material for extraction of vegetable oils, since they present great quantities of bioactive compounds. This study aimed to characterize the lipid fractions and the seeds of pumpkin (Cucurbita sp) varieties Nova Caravela, Mini Paulista, Menina Brasileira, and Moranga de Mesa aiming at using them in food. The chemical composition of the seeds was performed according to the official methods of American Oil Chemists' Society and Association of Official Analytical Chemists. Total carotenoids and phenolic compounds were determined by spectrophotometry, while the levels of tocopherols were analyzed by high efficiency liquid chromatography. It was noted that the seeds contain high amounts of macronutrients that are essential for the functioning of the human organism. As to total carotenoids, Mini Paulista and Menina Brasileira pumpkin varieties presented significant amounts, 26.80 and 26.03 μg/g, respectively. Mini Paulista and Nova Caravela pumpkin varieties showed high amounts of total phenolic compounds in the lipid fractions and in the seeds. It was also found that γ-tocopherol is the isomer that stood out in the lipid fractions and in the seeds, mainly in Menina Brasileira. Finally, the consumption of these seeds and use of lipid fractions provide the supply of large quantities of compounds that are beneficial for health and that may be potentially used in food, besides representing an alternative to better use of agro-industrial residues. Bioactive compounds, besides presenting basic nutritional functions, provide metabolic and physiological health benefits when consumed as part of the usual diet. Therefore, there is a growing interest in vegetable oils of special composition, such as the ones extracted from fruit seeds. The seeds of Cucurbita sp are shown to be promising sources of oils, and especially the Cucurbita moschata and maxima species have not yet

  3. Inherent lipid metabolic dysfunction in glycogen storage disease IIIa.

    Science.gov (United States)

    Li, Xin-Hua; Gong, Qi-Ming; Ling, Yun; Huang, Chong; Yu, De-Min; Gu, Lei-Lei; Liao, Xiang-Wei; Zhang, Dong-Hua; Hu, Xi-Qi; Han, Yue; Kong, Xiao-Fei; Zhang, Xin-Xin

    2014-12-05

    We studied two patients from a nonconsanguineous family with life-long abnormal liver function, hepatomegaly and abnormal fatty acid profiles. Abnormal liver function, hypoglycemia and muscle weakness are observed in various genetic diseases, including medium-chain acyl-CoA dehydrogenase (MCAD) deficiency and glycogen storage diseases. The proband showed increased free fatty acids, mainly C8 and C10, resembling fatty acid oxidation disorder. However, no mutation was found in ACADM and ACADL gene. Sequencing of theamylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL) gene showed that both patients were compound heterozygotes for c.118C > T (p.Gln40X) and c.753_756 del CAGA (p.Asp251Glufsx29), whereas their parents were each heterozygous for one of these mutations. The AGL protein was undetectable in EBV-B cells from the two patients. Transcriptome analysis demonstrated a significant different pattern of gene expression in both of patients’ cells, including genes involving in the PPAR signaling pathway, fatty acid biosynthesis, lipid synthesis and visceral fat deposition and metabolic syndrome. This unique gene expression pattern is probably due to the absence of AGL, which potentially accounts for the observed clinical phenotypes of hyperlipidemia and hepatocyte steatosis in glycogen storage disease type IIIa.

  4. PHYSIOLOGICAL CLASSIFICATION OF FOREST SEEDS REGARDING THE DESICCATION TOLERANCE AND STORAGE BEHAVIOUR

    Directory of Open Access Journals (Sweden)

    Rafaella Carvalho Mayrinck

    2016-03-01

    Full Text Available This work aims to classify forest seeds native to the Alto Rio Grande region regarding the desiccation tolerance and storage behaviour. Germination and water content tests were performed in seeds of different species. The tests were conducted immediately after seed processing, at 12% and 5% of water content, and at 5% after 3 months of storage in -18°C. Based on the results obtained, seeds were classified into recalcitrant, intermediate and orthodox class. Seeds of Brosimum gaudichaudii, Erythroxylum deciduum, Eugenia pleurantha, Myrcia venulosa, Nectandra megapotamica were classified as recalcitrant (22.7% of all species. Seeds of Aegiphila sellowiana, Aspidosperma parvifolium, Blepharocalyx salicifolius, Casearia lasiophylla, Cassia occidentalis, Dalbergia miscolobium, Diospyros brasiliensis, Diospyros hispida, Ilex brevicuspis, Ilex cerasifolia, Myrocarpus fastigiatus, Senna aversiflora, Senna splendida e Blepharocalyx salicifolius were classified as intermediate (59.1% of all species. Seeds of Miconia albicans, Platycyamus regnellii, Styrax camporum and Piptadenia gonoacantha were classified as orthodox (18.2% of all species.

  5. The effect of ethanolic seed extract of Cucurbita maxima on lipid ...

    African Journals Online (AJOL)

    Thirty (30) adult wistar rats were used to study the possible effects of ethanolic seed extract of Cucurbita maxima on lipid profile. The rats were divided randomly into two groups, that is, normal diet group and high fat diet group. Each group was further subdivided into three sub groups (A1,B1,C1 for the normal diet group and ...

  6. DIETARY BLACK CUMIN (NIGELLA SATIVA SEED OIL AFFECTS SERUM LIPIDS IN CHICKEN BROILERS

    Directory of Open Access Journals (Sweden)

    Aida Hodžić

    2012-07-01

    Full Text Available The research was performed on 40 chicken broilers of Cobb provenience to investigate the effect of dietary black cumin seed oil on some blood biochemical parameters in chicken broilers. 40 chicken broilers who were included in the 42 days lasting experiment were divided into two groups, each one of 20 individuals. Group K was the control – with no added oil in feed mixture and the second group P was the experimental one – chickens were fed with 0,025 g of p.o. administered black cumin seed oil. Feed and water supplies were ad libitum. Microclimate conditions (light, temperature and airflow were maintained according to the technological procedure. Blood samples were taken from the wing vein of all animals from both groups at age of 25, 32 and 39 days. The following blood serum biochemical parameters were determined: total lipids, triglycerides, total cholesterol, albumen and calcium. Dietary black cumin seed oil significantly (P<0.05 affected serum lipid components, particularly total lipids, but not albumen and calcium in chicken broilers. Possible reason for these findings could be dietary fat component as well as fatty-acid composition of added black cumin seed oil. Key words: black cumin seed oil, chicken broilers, blood serum biochemical parameters, fatty acid composition of oil

  7. Rab32 is important for autophagy and lipid storage in Drosophila.

    Directory of Open Access Journals (Sweden)

    Chao Wang

    Full Text Available Lipids are essential components of all organisms. Within cells, lipids are mainly stored in a specific type of organelle, called the lipid droplet. The molecular mechanisms governing the dynamics of lipid droplets have been little explored. The protein composition of lipid droplets has been analyzed in numerous proteomic studies, and a large number of lipid droplet-associated proteins have been identified, including Rab small GTPases. Rab proteins are known to participate in many intracellular membranous events; however, their exact role in lipid droplets is largely unexplored. Here we systematically investigate the roles of Drosophila Rab family proteins in lipid storage in the larval adipose tissue, fat body. Rab32 and several other Rabs were found to affect the size of lipid droplets as well as lipid levels. Further studies showed that Rab32 and Rab32 GEF/Claret may be involved in autophagy, consequently affecting lipid storage. Loss-of-function mutants of several components in the autophagy pathway result in similar effects on lipid storage. These results highlight the potential functions of Rabs in regulating lipid metabolism.

  8. Fatty acid composition of lipids in pot marigold (Calendula officinalis L.) seed genotypes.

    Science.gov (United States)

    Dulf, Francisc V; Pamfil, Doru; Baciu, Adriana D; Pintea, Adela

    2013-01-17

    Calendula officinalis L. (pot marigold) is an annual aromatic herb with yellow or golden-orange flowers, native to the Mediterranean climate areas. Their seeds contain significant amounts of oil (around 20%), of which about 60% is calendic acid. For these reasons, in Europe concentrated research efforts have been directed towards the development of pot marigold as an oilseed crop for industrial purposes. The oil content and fatty acid composition of major lipid fractions in seeds from eleven genotypes of pot marigold (Calendula officinalis L.) were determined. The lipid content of seeds varied between 13.6 and 21.7 g oil/100 g seeds. The calendic and linoleic acids were the two dominant fatty acids in total lipid (51.4 to 57.6% and 28.5 to 31.9%) and triacylglycerol (45.7 to 54.7% and 22.6 to 29.2%) fractions. Polar lipids were also characterised by higher unsaturation ratios (with the PUFAs content between 60.4 and 66.4%), while saturates (consisted mainly of palmitic and very long-chain saturated fatty acids) were found in higher amounts in sterol esters (ranging between 49.3 and 55.7% of total fatty acids). All the pot marigold seed oils investigated contain high levels of calendic acid (more than 50% of total fatty acids), making them favorable for industrial use. The compositional differences between the genotypes should be considered when breeding and exploiting the pot marigold seeds for nutraceutical and pharmacological purposes.

  9. Fatty acid composition of lipids in pot marigold (Calendula officinalis L. seed genotypes

    Directory of Open Access Journals (Sweden)

    Dulf Francisc V

    2013-01-01

    Full Text Available Abstract Background Calendula officinalis L. (pot marigold is an annual aromatic herb with yellow or golden-orange flowers, native to the Mediterranean climate areas. Their seeds contain significant amounts of oil (around 20%, of which about 60% is calendic acid. For these reasons, in Europe concentrated research efforts have been directed towards the development of pot marigold as an oilseed crop for industrial purposes. Results The oil content and fatty acid composition of major lipid fractions in seeds from eleven genotypes of pot marigold (Calendula officinalis L. were determined. The lipid content of seeds varied between 13.6 and 21.7 g oil/100 g seeds. The calendic and linoleic acids were the two dominant fatty acids in total lipid (51.4 to 57.6% and 28.5 to 31.9% and triacylglycerol (45.7 to 54.7% and 22.6 to 29.2% fractions. Polar lipids were also characterised by higher unsaturation ratios (with the PUFAs content between 60.4 and 66.4%, while saturates (consisted mainly of palmitic and very long-chain saturated fatty acids were found in higher amounts in sterol esters (ranging between 49.3 and 55.7% of total fatty acids. Conclusions All the pot marigold seed oils investigated contain high levels of calendic acid (more than 50% of total fatty acids, making them favorable for industrial use. The compositional differences between the genotypes should be considered when breeding and exploiting the pot marigold seeds for nutraceutical and pharmacological purposes.

  10. Seed origin, storage conditions, and gibberellic acid on in vitro ...

    African Journals Online (AJOL)

    It might help seed conservation and species propagation. The purpose of this study was to evaluate in vitro germination of guavira seeds collected from different sites and stored under different conditions. Also, the sowing of these seeds in MS medium supplemented with different concentrations of gibberellic acid (GA3) was ...

  11. Efficacy of traditional maize (Zea mays L.) seed storage methods in ...

    African Journals Online (AJOL)

    Efficacy of traditional maize (Zea mays L.) seed storage methods in western Kenya. ... PROMOTING ACCESS TO AFRICAN RESEARCH. AFRICAN JOURNALS ONLINE (AJOL) ... African Journal of Food, Agriculture, Nutrition and Development.

  12. Seed storage protein polymorphism in ten elite rice (Oryza sativa L ...

    African Journals Online (AJOL)

    user

    2011-02-14

    Feb 14, 2011 ... for several economical traits by conserving landrace genotypes and ... plasm, seed storage protein analysis represents a valid alternative ... of each variety was taken and ground into fine powder using pestle and mortal and ...

  13. [The composition of lipids and lipid peroxidation in the pancreas of quails exposed to nitrates and correction by the amaranth's seeds].

    Science.gov (United States)

    Tsekhmistrenko, S I; Ponomarenko, N V

    2013-01-01

    Researches of features of lipid composition, functioning of the system of antioxidant defense, maintenance of lipid peroxidation products in the quail's pancreas on the early postnatal ontogenesis stages are conducted for actions of nitrates and feeding with amaranth's seeds in mixed fodder. The arrival of nitrates in the organism of quails results in the decline of general lipids maintenance and nonetherified fat acids in the pancreas. Using of amaranth's seeds in mixed fodder on the background of the nitrate loading results in the increase of activity of the enzimes system of antioxidant defence, the growth of general lipid level in the quail's pancreas. Thus in correlation with separate classes of lipid maintenance of cholesterol goes down for certain, whereas the maintenance of triacylglycerols and ethers of cholesterol rises. The results obtained in the researches show the ability of amaranth's seeds to avert oxidative stress in quail's pancreas under nitrates influence.

  14. Pesticidal seed coats based on azadirachtin-A: release kinetics, storage life and performance.

    Science.gov (United States)

    Nisar, Keyath; Kumar, Jitendra; Arun Kumar, M B; Walia, Suresh; Shakil, Najam A; Parsad, Rajender; Parmar, Balraj S

    2009-02-01

    Infestation of seeds by pests during storage leads to deterioration in quality. Seed coating is an effective option to overcome the menace. Unlike synthetic fungicidal seed coats, little is known of those based on botanicals. This study aims at developing azadirachtin-A-based pesticidal seed coats to maintain seed quality during storage. Polymer- and clay-based coats containing azadirachtin-A were prepared and evaluated for quality maintenance of soybean seed during storage. Gum acacia, gum tragacanth, rosin, ethyl cellulose, hydroxyethyl cellulose, polyethyl methacrylate, methyl cellulose, polyethylene glycol, polyvinyl chloride, polyvinyl acetate, polyvinyl pyrrolidone and Agrimer VA 6 polymers and the clay bentonite were used as carriers. The time for 50% release (t(1/2)) of azadirachtin-A into water from the seeds coated with the different coats ranged from 8.02 to 21.36 h. The half-life (T(1/2)) of azadirachtin-A in the coats on seed ranged from 4.37 to 11.22 months, as compared with 3.45 months in azadirachtin-A WP, showing an increase by a factor of nearly 1.3-3.3 over the latter. The coats apparently acted as a barrier to moisture to reduce azadirachtin-A degradation and prevented proliferation of storage fungi. Polyethyl methacrylate, polyvinyl acetate and polyvinyl pyrrolidone were significantly superior to the other polymers. Azadirachtin-A showed a significant positive correlation with seed germination and vigour, and negative correlation with moisture content. Effective polymeric carriers for seed coats based on azadirachtin-A are reported. These checked seed deterioration during storage by acting as a barrier to moisture and reduced the degradation of azadirachtin-A.

  15. THE TYPE OF PACKAGING MATERIAL AND STORAGE CONDITIONS AS FACTORS FOR WHEAT SEED QUALITY

    Directory of Open Access Journals (Sweden)

    Josip Šimenić

    2000-12-01

    Full Text Available Seed of cereal is normally grown on 5-8% of the overall plots under cereals in the Republic of Croatia. The produced seed meets the needs for high quality seed of wheat, barley, oat and other cereals. Certain quantities of seed remain unsold every year and are kept at various storage conditions and in various packaging material. The objective of this paper was to find out which storage conditions and what sort of packaging material would provide for the best viability of wheat seed. The investigation was carried out at storage simulation and by using various packaging material. In addition to well-known packaging material, such as paper 2 and 4-layer bags, jute bags, and PPR bags, the seed was also packed in the PVC transparent and PVC black bags, as well as in bags made of Aluminium foil. The investigation lasted for two years and was carried out in three various storage conditions, such as in the "New Warehouse" - a warehouse of a new type with thermal isolation in the roof and with uncontrolled conditions, ii the "Old Warehouse" made of filled-in brick and with a roof made of asbestos board, and iii under the "Eaves". The results have shown that the best seed was obtained when packed in 2 and 4-layer paper bags, PVC transparent bags and those made of Aluminium foil. Poorer results were obtained with bags of jute, polypropeline bags and PVC black bags. The storage of seed at "Eaves" has attained the best results in both years of the investigation, as compared to all three types of storage and it can in our circumstances meet the needs for wheat seed storage during one year

  16. Characterization of myrtle seed (Myrtus communis var. baetica as a source of lipids, phenolics, and antioxidant activities

    Directory of Open Access Journals (Sweden)

    Wissem Aidi Wannes

    2016-04-01

    Full Text Available This study aimed to characterize the chemical composition and antioxidant activity of the oil and the methanolic extract of Myrtus communis var. baetica seed. The oil yield of myrtle seed was 8.90%, with the amount of neutral lipid, especially triacylglycerol, being the highest, followed by phospholipids and glycolipids. Total lipids and all lipid classes were rich in linoleic acid. The content of total phenols, flavonoids, tannins, and proanthocyanidins of the methanolic extract and the oil from myrtle seed was determined using spectrophotometric methods. Antioxidant activities of the oil and the methanolic extract from myrtle seed were evaluated using 1,1-diphenyl-2-picrylhydrazyl radical scavenging, β-carotene–linoleic acid bleaching, and reducing power and metal chelating activity assays. In all tests, the methanolic extract of myrtle seed showed better antioxidant activity than oil. This investigation could suggest the use of myrtle seed in food, industrial, and biomedical applications for its potential metabolites and antioxidant abilities.

  17. Characterization of volatile production during storage of lettuce (Lactuca sativa) seed.

    Science.gov (United States)

    Mira, Sara; González-Benito, M Elena; Hill, Lisa M; Walters, Christina

    2010-09-01

    The duration that seeds stay vigorous during storage is difficult to predict but critical to seed industry and conservation communities. Production of volatile compounds from lettuce seeds during storage was investigated as a non-invasive and early detection method of seed ageing rates. Over 30 volatile compounds were detected from lettuce seeds during storage at 35 degrees C at water contents ranging from 0.03 to 0.09 g H(2)O g(-1) dw. Both qualitative and quantitative differences in volatile composition were noted as a function of water content, and these differences were apparent before signs of deterioration were visible. Seeds stored at high water content (L >or=0.06 g H(2)O g(-1) dw) emitted molecular species indicative of glycolysis (methanol+ethanol), and evidence of peroxidation was apparent subsequent to viability loss. Seeds containing less water (0.03-0.05 g H(2)O g(-1) dw) produced volatiles indicative of peroxidation and survived longer compared with seeds stored under more humid conditions. Production of glycolysis-related by-products correlated strongly with deterioration rate when measured as a function of water content. This correlation may provide a valuable non-invasive means to predict the duration of the early, asymptomatic stage of seed deterioration.

  18. Effects of storage structures and moisture contents on seed quality attributes of quality protein maize

    Directory of Open Access Journals (Sweden)

    Gopal Bhandari

    2017-12-01

    Full Text Available The study was aimed to examine the effects of various storage structures and moisture contents on seed quality attributes of quality protein maize seed. The quality protein maize (QPM-1 seed was tested in conventional seed storage containers (Fertilizer sack and earthen pot and the improved hermetic ones (Metal bin, Super grain bag, and Purdue Improved Crop Storage (PICS bag at Seed Science and Technology Division, Khumaltar, Nepal during February, 2015 to January 2016. Ten treatments comprising 5 storage devices in two moisture regimes (11% and 9% replicated thrice and laid out in Completely Randomized Design (CRD. Data on temperature, relative humidity (RH, germination, electrical conductivity (EC, seed moisture content (MC were collected bimonthly. The conventional containers were found liable to the external environmental condition whereas the hermetic structures observed with controlled RH level below 40% in all combinations. Electrical conductivity (EC for seed vigor showed that hermetic containers provide higher seed vigor than the conventional ones. Up to 4 months all treatments were found statistically at par for germination. A significant difference was observed in each treatment after 4 months where PICS bag & Super grain bag showed best germination followed by metal bin while fertilizer bag & earthen-pot showed poorer and poorest germination respectively till one year. Almost all treatments with lower MC showed better results than the treatments with higher MC. A negative correlation (R2=69.7% was found between EC and Germination. All six figures from 2 to 12 months on MC showed statistically different where hermetic plastic bags were found maintaining MC as initial whereas MC of fertilizer bags and earthen pot was spiked than the basal figure. The finding evidenced that the hermetic containers and low MC are the seed storage approaches for retaining the quality of seed even in an ambient environmental condition for more than a year.

  19. A novel PNPLA2 mutation causes neutral lipid storage disease with myopathy (NLSDM) presenting muscular dystrophic features with lipid storage and rimmed vacuoles.

    Science.gov (United States)

    Chen, J; Hong, D; Wang, Z; Yuan, Y

    2010-01-01

    Neutral lipid storage disease with myopathy (NLSDM) is a type of lipid storage myopathy arising due to a mutation in the PNPLA2 gene encoding an adipose triglyceride lipase responsible for the degradation of intracellular triglycerides. Herein, we report the cases of two siblings manifesting slowly progressive proximal and distal limb weakness in adulthood. One of the patients had dilated cardiomyopathy, hearing loss and short stature. Muscle specimens of the 2 patients revealed muscular dystrophic features with massive lipid droplets and numerous rimmed vacuoles in the fibers. A novel homozygous mutation IVS2+1G > A in the PNPLA2 gene was identified in the 2 cases, but not in the healthy familial individuals. The presence of massive lipid droplets with muscular dystrophic changes and rimmed vacuoles in muscle fibers might be one of the characteristic pathological changes of NLSDM.

  20. Effects of accelerated aging upon the lipid composition of seeds from two soft wheat varieties from Morocco

    Energy Technology Data Exchange (ETDEWEB)

    Ouzouline, M.; Tahani, N.; Demandre, C.; El Amrani, A.; Benhassaine-Kesri, G.; Serghini Caid, H.

    2009-07-01

    The lipid composition of the seeds from two soft wheat varieties (Triticum aestivum, cv. Marchouche and Mahdia) were analyzed before and after accelerated aging. Eight days of accelerated aging resulted in a total inhibition of seed germ inability as well as a decrease in their total and especially unsaturated fatty acid contents. Oleic and linoleic acid contents decreased particularly in the phosphatidylcholine of the seeds from both varieties. The proportion of polar lipids also decreased after aging as compared to neutral lipids: a 5.8% and 7.2% decrease in polar lipids was e observed in Mahdia and Marchouche cultivars, respectively. In the neutral lipids of the seeds from the Marchouche variety, the percentage of free fatty acids increased whereas the triacylglycerols decreased. After aging, the fatty acid compositions of all lipid classes were modified in the same manner as total fatty acid compositions. Among polar lipids, phospholipid proportions were particularly small, especially the phosphatidylcholine percentages with an 18.1% and 19.1% decrease in Mahdia and Marchouche varieties, respectively. In contrast, MGDG percentages increased, especially in the seeds from the Marchouche variety. A 15.5% increase was noticed when compared with seeds which were not aged. At the same time, the DGDG percentage showed a 16.6% decrease after accelerated aging of the seeds from the Marchouche variety. From these results we concluded that the lipid content decrease observed in seeds after accelerated aging could be linked to a loss in the germination and vigor of wheat seeds. (Author) 38 refs.

  1. Changes in non-enzymatic antioxidant capacity and lipid peroxidation during germination of white, yellow and purple maize seeds

    International Nuclear Information System (INIS)

    Deng, B.; Zhang, Y.; Yang, K.

    2016-01-01

    In this study, the changes in non-enzymatic antioxidant capacity and lipid peroxidation during the germination process of purple, yellow and white maize seeds were compared, under favorable conditions. Results showed that germination can increase non-enzymatic antioxidant capacity (evaluated with ferric reducing power and 2, 2-diphenyl-1-picryl-hydrazyl-hydrate radical scavenging capacity) and lipid peroxidation levels for all these seeds. In addition, non-enzymatic antioxidant capacity observed in the germinating seeds were in the order of purple > yellow > white. However, the highest and lowest levels of lipid peroxidation could be seen during the germination processes of the white and purple seeds, respectively. In addition, the germination rates of the seeds followed the order of white > yellow > purple. Further studies showed that H/sub 2/O/sub 2/ treatment can significantly promote seed germination, especially for purple seeds. In addition, DMTU (dimethylthiourea), a specific scavenger for H/sub 2/O/sub 2/, could slightly but significantly arrest dormancy release. Data analysis showed that a high negative correlation (R/sup 2/ = -0.955) existed between non-enzymatic antioxidant capacity and germination rates. However, a high positive correlation (R/sup 2/ = 0.860) could be detected between lipid peroxidation and germination rates. Finally, lipid peroxidation as a possible novel signaling mechanism for seed germination has been discussed under stress-free conditions. (author)

  2. Biorhythms in conifer seed germination during extended storage

    Science.gov (United States)

    James P. Barnett; N.I. Marnonov

    1989-01-01

    A proportion of sound seeds of conifer species do not germinate during certain periods of the year, even when conditions are favorable. Mamonov et al. (1986) report that the non-germinating seeds have apparently undergone physiological changes that affected germination. This phenomenon may be due to seasonal periodicity, or biorhythms. As early as the mid-1930'...

  3. Shaping of the fungal communities isolated from yellow lupin seeds (Lupinus luteus L. throughout storage time

    Directory of Open Access Journals (Sweden)

    Bożena Cwalina-Ambroziak

    2012-12-01

    Full Text Available The object of the experiment were seeds of two traditional cultivars of yellow lupin (Juno and Amulet cultivated in 1999 in two crop-rotation with 20% and 33% yellow lupine contribution. The quantitative and qualitative composition of the fungal community colonizing the seeds were determined in the laboratory conditions after 0.5-, 1.5- and 2.5-year of storage time. In total 1077 fungal colonies were isolated from the lupin seeds. Fungi representing the species of Penicillium - 29.3%, Alternaria alternata - 26.7% and Rhizopus nigricans - 12.7% were isolated most widely. Among the fungi pathogenic to lupin, the species of Colletotrichum gloeosporioides (16.3% isolates was dominant. The crop rotation with 20% lupin reduced the number of fungal colonies colonizing the seeds including the pathogens from the species of C. gloeosporioides. Seed disinfection decreased the total number of fungal colonies isolated from both cultivars. Higher number of C. gloeosporioides isolates was found in the combination with disinfected seeds. More fungal colonies were obtained from seeds of cv. Amulet than from those of cv. Juno. The storage duration had an effect on the population and the composition of species of fungi isolated from seeds of yellow lupine. With longer storage population of Penicillium spp. and Rhizopus spp. increased, whereas the population of C. gloeosporioides decreased.

  4. Electrical conductivity testing of corn seeds as influenced by temperature and period of storage

    OpenAIRE

    Fessel,Simone Aparecida; Vieira,Roberval Daiton; Cruz,Mara Cristina Pessoa da; Paula,Rinaldo Cesar de; Panobianco,Maristela

    2006-01-01

    The objective of this work was to evaluate the effects of temperature (10, 20, 30, 20/10 and 30/10ºC) and period of storage on electrical conductivity (EC) in four seed lots of corn (Zea mays L.), as well as the mineral composition of the soaking solution. EC test determines indirectly the integrity of seed membrane systems, and is used for the assessment of seed vigor, because this test detects the seed deterioration process since its early phase. The research comprised determinations o...

  5. Studies on the lipid constituents of grape seeds recovered from pomace resulting from white grape processing

    OpenAIRE

    El-Shami, S. M.; El-Mallah, M. H.; Mohamed, S. S.

    1992-01-01

    Studies on the lipid constituents of grape seeds, produced as by-product from white grape processing, were carried out. Gas liquid chromatography was used to determine the composition of fatty acid methyl esters and sterol silyl derivatives of the oil. The oil was found to contain appreciable amount of unsaturated fatty acids, namely, oleic and linoleic acids, however, linolenic acid was not detected. Saturated fatty acids, namely, palmitic and stearic were found to be present in reasonable a...

  6. Reserve carbohydrates and lipids from the seeds of four tropical tree species with different sensitivity to desiccation

    Directory of Open Access Journals (Sweden)

    Juliana Iura de Oliveira Mello

    2010-08-01

    Full Text Available Considering the importance of water content for the conservation and storage of seeds, and the involvement of soluble carbohydrates and lipids for embryo development, a comparative study was carried out among the seeds of Inga vera (ingá, Eugenia uniflora (pitanga, both classified as recalcitrant, and Caesalpinia echinata (brazilwood and Erythrina speciosa (mulungu, considered as orthodox seeds. Low concentrations of cyclitols (0.3-0.5%, raffinose family oligosaccharides (ca. 0.05% and unsaturated fatty acids (0-19% were found in the seeds of ingá and pitanga, while larger amounts of cyclitols (2-3% and raffinose (4.6-13% were found in brazilwood and mulungu, respectively. These results, in addition to higher proportions of unsaturated fatty acids (53-71% in orthodox seeds, suggested that sugars and lipids played important role in water movement, protecting the embryo cell membranes against injuries during dehydration.Os compostos de reserva das sementes, além de suprirem energia para o desenvolvimento embrionário, desempenham importantes funções relacionadas à proteção celular contra secagem e congelamento. Considerando a importância do teor de água para a conservação e para o armazenamento de sementes de espécies arbóreas e o envolvimento dos carboidratos solúveis e de lipídios nesses processos, foi realizada uma análise comparativa desses compostos em sementes de Inga vera (ingá e Eugenia uniflora (pitanga, consideradas recalcitrantes, e em Caesalpinia echinata (pau-brasil e Erythrina speciosa (mulungu, com comportamento ortodoxo. Baixas concentrações de ciclitóis (0,3-0,5%, de oligossacarídeos da série da rafinose (0,05% e de ácidos graxos insaturados (0-19% foram encontradas em sementes de ingá e pitanga, enquanto maiores quantidades de ciclitóis (2-3% e de rafinose (4,6-13% foram encontradas nas sementes de pau-brasil e mulungu, respectivamente. Estes resultados, juntamente com as altas proporções de

  7. Blocked muscle fat oxidation during exercise in neutral lipid storage disease

    DEFF Research Database (Denmark)

    Laforêt, Pascal; Ørngreen, Mette; Preisler, Nicolai

    2012-01-01

    To determine whether impaired exercise capacity in neutral lipid storage disease with myopathy is solely caused by muscle weakness or whether a defect in energy metabolism (blocked fat oxidation) may also play a role.......To determine whether impaired exercise capacity in neutral lipid storage disease with myopathy is solely caused by muscle weakness or whether a defect in energy metabolism (blocked fat oxidation) may also play a role....

  8. Pine seeds radiosensitivity as depended upon their humidity and the term of storage after radiation exposure

    International Nuclear Information System (INIS)

    Porozova, O.A.

    1983-01-01

    The seeds of pine (Pinus silvestris L.) have been used to study the effect of average and so called ''low doses'' of ionizing radiation at different levels of seed humidity (3-4%-dried a little, 6-7%-aerially-dry and 9-10%-humid). The Seeds of every humidity level are irradiated in May in a rather wide dose range 0.5, 1, 2, 5, 10, 20, 30 Gy from a gamma source of 137 Cs at the dose rate of 0.96 Gy/min. After irradiation part of seeds was sown. It is shown that seeds with relatively high water content are more stable to the effect of gamma-radiation. The first indices of radiation injury in humid seeds (9-10% humidity) in the criteria of laboratory growing-out are noticed at the dose of 20 Gy while in dried seeds (3-4% hymidity) - at the dose of 2 Gy. Radiation injury of aerially-dried and dried seeds increases with the increase of radiation dose and periods of their storage. In humid seeds this effect depends on radiation dose alone; their storage for eight months did not produce a noticeable effect on growing-out

  9. Lipid composition of seed oils of different pomegranate (Punica granatum L. cultivars from Spain

    Directory of Open Access Journals (Sweden)

    Luana Fernandes

    2015-04-01

    Full Text Available Pomegranate (Punica granatum L. is an ancient fruit tree traditionally cultivated in the Near and Middle East. Presently, its most important growing regions include Afghanistan, Iran, Israel, USA, Italy and Spain, the latter country the largest European exporter. The pomegranate fruit can be divided into several anatomical compartments: outside peel, inside peel, and arils (pulp and seeds, the last part being usually used for consumption as is or for juice, jams and jellies production. Even though pomegranate seeds are an industrial by-product, recent reports have highlighted their potential use as a source of oil with beneficial chemical attributes. Therefore, the main objective of the present work was to characterize the seed oil of nine European pomegranate varieties, collected in Spain, for their fatty acid and vitamin E compositions. All seed lipid fractions consisted mainly of punicic acid (c9,t11,c13 C-18:3, ranging between 77.3% and 83.6% of total fatty acids, followed by small amounts of linoleic acid (C18:2n6, oleic acid (C18:1n9 and palmitic acid (C16:0. Regarding vitamin E composition, α-, γ-, δ-tocopherols were found in all pomegranate seed oils, but mainly γ-tocopherol, with total tocopherols ranging from 174.5 to 627.3 mg/100g oil.The richness of these pomegranate varieties seed oils in punicic acid, a conjugated linolenic acid with interesting anti-carcinogenic activity, and the elevated amount of tocopherols on the extracted lipids, of technological and nutritional relevance, make this by-product interesting for further exploitation.

  10. Effect of Grape Seeds Oil Extracted from Radiation Processed Seeds on Lipid Metabolism and on Antioxidant Activity in Rats Fed Diets Containing Cholesterol

    International Nuclear Information System (INIS)

    El-Neily, H.F.G.; El-Shennawy, H.M.

    2011-01-01

    Grape seeds were separated from fresh grape pomace and dried at room temperature then packed in polyethylene bags and subjected to gamma rays at dose level of 10 kGy. The grape seeds oil was extracted from non and irradiated seeds. The oil quality, fatty acid composition and total phenolic compounds of oil extracted from non or irradiated seeds have been studied. The results indicated that there were significant increases in the acid value, saponification value and peroxide value of oil extracted from irradiated seeds at 10 kGy by 46.2%, 2.5% and 95.2%, respectively, and the total phenolic compounds and total radical trapping antioxidant potential (TRAP) were reduced by 22.13% and 10%, respectively, as compared to those of oil extracted from non-irradiated seeds. No degradation of the fatty acids; palmitic, linoleic, linolenic and arachidic acids, were observed for oil extracted from irradiated seeds at 10 kGy. However, significant decrease in oleic acid by 11.35% and increase in stearic acid by 26.22% were recorded corresponding to those for oil extracted from non-irradiated seeds. The effect of grape seeds oils extracted from non or irradiated seeds on lipid metabolism and antioxidant activity was investigated using 60 male Albino rats divided into six groups: (1) Control group: animals fed casein diet. (2) Ch group: animals received casein diet contains 10 g cholesterol per kg diet. (3) RGSO group: animals received diet contains grape seeds oil extracted from non-irradiated seeds (100 g oil per kg diet). (4) RGSO + Ch group: rats received diet contains grape seeds oil extracted from non-irradiated seeds (100 g oil per kg diet) + 100 g cholesterol per kg diet. (5) IGSO group: rats received diet contains grape seeds oil extracted from irradiated seeds at 10 kGy (100 g oil per kg diet). (6) IGSO + Ch group: rats received diet contains grape seeds oil extracted from irradiated seeds at 10 kGy (100 g oil per kg diet) + 10 g cholesterol per kg diet. Animals received

  11. Effect of hybrid, storage conditions and seed protection on sunflower field emergence

    Directory of Open Access Journals (Sweden)

    Mrđa Jelena

    2010-01-01

    Full Text Available Seed emergence under field conditions decisively and directly determines the number of plants per hectare, which is one of three basic components of yield in the plant world. Influence of chemical treatment on field emergence of three commercial sunflower hybrids stored in different conditions was tested in 2007 and 2008 on experimental field of Institute of Field and Vegetable Crops in Novi Sad. On average, hybrid H1 had the highest value of field emergence (88.79% and for chemical treatment fl udioxonil + metalaxyl + imidacloprid (87.71%. Seed kept in common storage had the highest emergence value in fi eld (87.92%. Seed treated with fl udioxonil + metalaxyl + imidacloprid and stored for one year in common storage had the highest field emergence (90.18%. Considering interaction between storage conditions and genotype, hybrid H1 seed sown after chemical treatment had the highest field emergence (91.82% and seed kept in common storage (90.48%. Hybrid H1 seed compared with other two had the highest field emergence treated with fludioxonil + metalaxyl + imidacloprid (91.84%.

  12. Extraction of lipid components from hibiscus seeds by supercritical carbon dioxide and ethanol mixtures

    Energy Technology Data Exchange (ETDEWEB)

    Holser, Ronald A.; King, J. W. (Jerry W.); Bost, G.

    2002-01-01

    The genus Hibiscus exhibits great diversity in the production of natural materials with edible and industrial applications. The seeds of twelve varieties of Hibiscus were investigated as a source for triglycerides and phospholipids that could be used in functional foods. Lipid components were extracted from seed samples ground to a nominal particle diameter of 0.1 mm. Extractions were performed with an ISCO model 3560 supercritical fluid extractor using carbon dioxide and a mixture of carbon dioxide modified with ethanol. The neutral lipids were extracted with carbon dioxide at 80 C and 5370 MPa for 45 min. Polar lipids were subsequently extracted with a mixture of carbon dioxide and 15% ethanol at the same temperature and pressure. High performance liquid chromatography (HPLC) was used to analyze extracts for major neutral and polar lipid classes. A silica column was used with a solvent gradient of hexane/isopropanol/ water and ultraviolet (UV) and evaporative light scattering detectors (ELSD). An aliquot of each triglyceride fraction was trans-methylated with sodium methoxide and analyzed by gas chromatography to obtain the corresponding fatty acid methyl esters. The total lipids extracted ranged from 8.5% for a variety indigenous to Madagascar (H. calyphyllus) to 20% for a hybrid species (Georgia Rose). The average oil yield was 11.4% for the other varieties tested. The fatty acid methyl ester analysis displayed a high degree of unsaturation for all varieties tested, e. g., 75 ' 83%. Oleic, linoleic, and linolenic fatty acids were the predominate unsaturated fatty acids with only minor amounts of C14, C18, and C20 saturated fatty acids measured. Palmitic acid was identified as the predominate saturated fatty acid. The distribution of the major phospholipids, i. e., phosphatidylethanolamine, phosphatidic acid, phosphatidylserine, phosphatidylcholine, and lysophosphatidylcholine, was found to vary significantly among the hibiscus species examined

  13. Co-ordinate regulation of genes involved in storage lipid mobilization in Arabidopsis thaliana.

    Science.gov (United States)

    Rylott, E L; Hooks, M A; Graham, I A

    2001-05-01

    Molecular genetic approaches in the model plant Arabidopsis thaliana (Col0) are shedding new light on the role and control of the pathways associated with the mobilization of lipid reserves during oilseed germination and post-germinative growth. Numerous independent studies have reported on the expression of individual genes encoding enzymes from the three major pathways: beta-oxidation, the glyoxylate cycle and gluconeogenesis. However, a single comprehensive study of representative genes and enzymes from the different pathways in a single plant species has not been done. Here we present results from Arabidopsis that demonstrate the co-ordinate regulation of gene expression and enzyme activities for the acyl-CoA oxidase- and 3-ketoacyl-CoA thiolase-mediated steps of beta-oxidation, the isocitrate lyase and malate synthase steps of the glyoxylate cycle and the phosphoenolpyruvate carboxykinase step of gluconeogenesis. The mRNA abundance and enzyme activities increase to a peak at stage 2, 48 h after the onset of seed germination, and decline thereafter either to undetectable levels (for malate synthase and isocitrate lyase) or low basal levels (for the genes of beta-oxidation and gluconeogenesis). The co-ordinate induction of all these genes at the onset of germination raises the possibility that a global regulatory mechanism operates to induce the expression of genes associated with the mobilization of storage reserves during the heterotrophic growth period.

  14. Comparative study of lipids in mature seeds of six Cordia species (family Boraginaceae) collected in different regions of Brazil.

    Science.gov (United States)

    Carvalho, Patricía de O; Arrebola, Melissa B; Sawaya, Alexandra C H F; Cunha, Ildenize B S; Bastos, Deborah H M; Eberlin, Marcos N

    2006-08-01

    The oil content, FA, and lipid class composition of the mature seeds of six Cordia species were analyzed. Mature seeds of each species were collected in their natural habitat from 2002 to 2004. The total lipid content varied from 1.9% to 13.2%, there being significant differences between the results found in different years for each species and between the species analyzed. The contents of FFA varied from 2.0% to 7.9% of total lipids. Neutral lipids (NL) were the largest class, making up between 89.6% and 96.4% of the total lipids; the phospholipids (PL) were the second largest class (3.0% to 8.9% of the total lipids), and the glycolipids (GL) were the smallest class (0.6 to 3.4%). The presence of GLA was determined in each class of lipids; it is predominant in the NL. Levels of GLA ranged from 1.2% to 6.8% of total seed FA. This is, to our knowledge the first study of lipid composition in seeds of species of Cordia from Brazil.

  15. Aberrant hepatic lipid storage and metabolism in canine portosystemic shunts.

    Directory of Open Access Journals (Sweden)

    Lindsay Van den Bossche

    Full Text Available Non-alcoholic fatty liver disease (NAFLD is a poorly understood multifactorial pandemic disorder. One of the hallmarks of NAFLD, hepatic steatosis, is a common feature in canine congenital portosystemic shunts. The aim of this study was to gain detailed insight into the pathogenesis of steatosis in this large animal model. Hepatic lipid accumulation, gene-expression analysis and HPLC-MS of neutral lipids and phospholipids in extrahepatic (EHPSS and intrahepatic portosystemic shunts (IHPSS was compared to healthy control dogs. Liver organoids of diseased dogs and healthy control dogs were incubated with palmitic- and oleic-acid, and lipid accumulation was quantified using LD540. In histological slides of shunt livers, a 12-fold increase of lipid content was detected compared to the control dogs (EHPSS P<0.01; IHPSS P = 0.042. Involvement of lipid-related genes to steatosis in portosystemic shunting was corroborated using gene-expression profiling. Lipid analysis demonstrated different triglyceride composition and a shift towards short chain and omega-3 fatty acids in shunt versus healthy dogs, with no difference in lipid species composition between shunt types. All organoids showed a similar increase in triacylglycerols after free fatty acids enrichment. This study demonstrates that steatosis is probably secondary to canine portosystemic shunts. Unravelling the pathogenesis of this hepatic steatosis might contribute to a better understanding of steatosis in NAFLD.

  16. A novel mutation in PNPLA2 leading to neutral lipid storage disease with myopathy.

    Science.gov (United States)

    Ash, Daniel B; Papadimitriou, Dimitra; Hays, Arthur P; Dimauro, Salvatore; Hirano, Michio

    2012-09-01

    Mutations in PNPLA2, a gene encoding adipose triglyceride lipase, lead to neutral lipid storage disease with myopathy. To report the clinical and molecular features of a case of neutral lipid storage disease with myopathy resulting from a novel mutation in PNPLA2. Case report. University hospital. A 65-year-old man with progressive muscle weakness and high serum creatine kinase levels. Direct sequencing of the PNPLA2 gene. Identification of a novel homozygous mutation in the patient's PNPLA2 gene confirmed the suspected diagnosis of neutral lipid storage disease with myopathy. Screening of the PNPLA2 gene should be considered for patients presenting with high levels of creatine kinase, progressive muscle weakness, and systemic lipid accumulation. The presence of Jordans anomaly can be a strong diagnostic clue.

  17. Effects of plant polyphenols and a-tocopherol on lipid oxidation, residual nitrites, biogenic amines, and N-nitrosamines formation during ripening and storage of dry-cured bacon

    Science.gov (United States)

    Effects of plant polyphenols (green tea polyphenols (GTP) and grape seed extract (GSE) and a-tocopherol on physicochemical parameters, lipid oxidation, residual nitrite, microbiological counts, biogenic amines, and N-nitrosamines were determined in bacons during dry-curing and storage. Results show ...

  18. Genetics Home Reference: neutral lipid storage disease with myopathy

    Science.gov (United States)

    ... named? Additional Information & Resources MedlinePlus (6 links) Encyclopedia: Hypothyroidism Encyclopedia: Type 2 Diabetes Health Topic: Cardiomyopathy Health Topic: Lipid Metabolism Disorders Health Topic: Muscle Disorders Health Topic: Pancreatitis Genetic and Rare Diseases ...

  19. Aberrant hepatic lipid storage and metabolism in canine portosystemic shunts.

    Science.gov (United States)

    Van den Bossche, Lindsay; Schoonenberg, Vivien A C; Burgener, Iwan A; Penning, Louis C; Schrall, Ingrid M; Kruitwagen, Hedwig S; van Wolferen, Monique E; Grinwis, Guy C M; Kummeling, Anne; Rothuizen, Jan; van Velzen, Jeroen F; Stathonikos, Nikolas; Molenaar, Martijn R; Helms, Bernd J; Brouwers, Jos F H M; Spee, Bart; van Steenbeek, Frank G

    2017-01-01

    Non-alcoholic fatty liver disease (NAFLD) is a poorly understood multifactorial pandemic disorder. One of the hallmarks of NAFLD, hepatic steatosis, is a common feature in canine congenital portosystemic shunts. The aim of this study was to gain detailed insight into the pathogenesis of steatosis in this large animal model. Hepatic lipid accumulation, gene-expression analysis and HPLC-MS of neutral lipids and phospholipids in extrahepatic (EHPSS) and intrahepatic portosystemic shunts (IHPSS) was compared to healthy control dogs. Liver organoids of diseased dogs and healthy control dogs were incubated with palmitic- and oleic-acid, and lipid accumulation was quantified using LD540. In histological slides of shunt livers, a 12-fold increase of lipid content was detected compared to the control dogs (EHPSS Plipid-related genes to steatosis in portosystemic shunting was corroborated using gene-expression profiling. Lipid analysis demonstrated different triglyceride composition and a shift towards short chain and omega-3 fatty acids in shunt versus healthy dogs, with no difference in lipid species composition between shunt types. All organoids showed a similar increase in triacylglycerols after free fatty acids enrichment. This study demonstrates that steatosis is probably secondary to canine portosystemic shunts. Unravelling the pathogenesis of this hepatic steatosis might contribute to a better understanding of steatosis in NAFLD.

  20. EFFECTS OF STORAGE PERIODS AND METHODS OF OVERCOMING DORMANCY IN SEEDS OF PASSIFLORA

    Directory of Open Access Journals (Sweden)

    Telma Miranda dos Santos

    2012-07-01

    Full Text Available The aim of this work was to evaluate the effects of storage period and treatement with hot water at 50ºC or scarification on dormancy break down on Passiflora mucronata seeds. The storage periods were 0, 1, 4 and 12 months. The seeds were sown onto Germitest paper roll and incubated in a germination chamber under 20ºC/8h-30ºC/16h alternate temperature, at 16-h photoperiod (fluorescent light at 32 mol m-2 s-1 irradiance. The percent germination was evaluated, and the seeds germinated on moistened Germitest paper rolls in distilled water at a ratio of two and a half times the paper weight. The seeds were transferred to a germination chamber with alternating temperatures of 20-30º C and photoperiod of 16 h until the end of the experiment at 31 days. The experiment was analyzed as completely randomized designed with four replications, in which each plot was constituted by 50 seeds. The storage periods had significant effect on the variable studied, where the higher germination was obtained at freshly harvested seeds. At the first month of evaluation the germination decreased. After 4 and 12 months of storage, no germination was detected. The treatment with hot water at 50º or scarification favored the germination of the Passiflora seeds stored by one and four months, however even with the stimulus of the treatments, the values of final germination were low. The treatments were not efficient to stimulate the seed germination stored by 12 months.

  1. Embryo-specific expression of soybean oleosin altered oil body morphogenesis and increased lipid content in transgenic rice seeds.

    Science.gov (United States)

    Liu, Wen Xian; Liu, Hua Liang; Qu, Le Qing

    2013-09-01

    Oleosin is the most abundant protein in the oil bodies of plant seeds, playing an important role in regulating oil body formation and lipid accumulation. To investigate whether lipid accumulation in transgenic rice seeds depends on the expression level of oleosin, we introduced two soybean oleosin genes encoding 24 kDa proteins into rice under the control of an embryo-specific rice promoter REG-2. Overexpression of soybean oleosin in transgenic rice leads to an increase of seed lipid content up to 36.93 and 46.06 % higher than that of the non-transgenic control, respectively, while the overall fatty acid profiles of triacylglycerols remained unchanged. The overexpression of soybean oleosin in transgenic rice seeds resulted in more numerous and smaller oil bodies compared with wild type, suggesting that an inverse relationship exists between oil body size and the total oleosin level. The increase in lipid content is accompanied by a reduction in the accumulation of total seed protein. Our results suggest that it is possible to increase rice seed oil content for food use and for use as a low-cost feedstock for biodiesel by overexpressing oleosin in rice seeds.

  2. Soluble carbohydrates in cereal (wheat, rye, triticale seed after storage under accelerated ageing conditions

    Directory of Open Access Journals (Sweden)

    Agnieszka I. Piotrowicz-Cieślak

    2011-01-01

    Full Text Available Germinability and the content of soluble carbohydrates were analysed in cereal seed (winter rye, cv. Warko; spring wheat, cv. Santa; hexaploid winter triticale, cv. Fidelio and cv. Woltario. Seed moisture content (mc was equilibrated over silica gel to 0.08 g H2O/g dry mass and stored in a desiccator at 20oC for up to 205 weeks or were equilibrated to mc 0.06, 0.08 or 0.10 g H2O/g dm and subjected to artificial aging at 35oC in air-tight laminated aluminium foil packages for 205 weeks. It was shown that the rate of seed aging depended on the species and seed moisture content. The fastest decrease of germinability upon storage was observed in seed with the highest moisture level. Complete germinability loss for winter rye, winter triticale cv. Fidelio, winter triticale cv. Woltario and spring wheat seed with mc 0.10 g H2O/g dm3 occurred after 81, 81, 101 and 133 weeks, respectively. Fructose, glucose, galactose, myo-inositol, sucrose, galactinol, raffinose, stachyose and verbascose were the main soluble carbohydrates found in the seed. The obtained data on the contents of specific sugars and the composition of soluble sugars fraction in seed of rye, wheat and triticale did not corroborate any profound effect of reducing sugars, sucrose and oligosaccharides on seed longevity.

  3. Surface sterilization and duration of seed storage influenced ...

    African Journals Online (AJOL)

    AJB SERVER

    2006-08-03

    Aug 3, 2006 ... probably due to little or no compaction of the potting medium (ricehull). A previous study (Baiyeri, 2003) and a more recent one (Baiyeri and Mbah, 2006), using soil- based and soilless media recorded relatively lower percent seedling emergence, probably because germinated seeds in those studies could ...

  4. Cytogenetic effects of electron-beam radiation on dry seed storage

    International Nuclear Information System (INIS)

    Baojiang, G.; Qishen, P.; Kohlman, A.

    1989-01-01

    Dry seeds of Viciafaba were exposed to 5 MeV electron beam (10–30 Krad) and stored afterwards during 20,40 and 60 days- Induction of chromosomal aberrations in root-tip cells of irradiated seeds has been found dose-dependent. The frequency of chromosomal aberrations (particularly, the bridges and the rings) and the frequency of micronucleated cells is proportional to the length of storage time, but is not significantly influenced by low temperatures (0–6°C) during storage. (author)

  5. Storage on the vigor and viability of macauba seeds from two provenances of Minas Gerais State

    Directory of Open Access Journals (Sweden)

    Patrícia Pereira de Souza

    Full Text Available ABSTRACT: Macauba palm stands out for having favorable features to biodiesel production such as the high oil content of its fruit. Considering the great potential of the species and their applicability in the renewable energy field, it becomes indispensable to establish the right conditions for storing the seeds for propagation purpose. The aim of this research was to evaluate the effect of seed moisture content, packaging, and storage conditions such as temperature and relative humidity on the quality of seeds from Minas Gerais State, during a 12-month storage period. The research had two independent assays: (I the seeds were stored with three moisture contents/ranges 4.0≤6.0%; 6.0≤8.0% and 8.0≤10.0% in impermeable packages, under room temperature and at 10ºC; (II seeds with approximately 5.9% of moisture content were stored in three different types of packages: a permeable, b semi-permeable and c impermeable. Three storing conditions were tested: a room temperature and RH under laboratory conditions; b 15ºC and 45% RH; c 20ºC and 55% RH. Water content, germination rate and germination speed index were evaluated at 0, 4, 8 and 12 months of storing. The best germination results were obtained with the moisture range of 6.0≤8.0%, with seeds kept at room temperature; while the seeds stored at 10ºC, regardless the moisture range, did not survive. The stored seeds with 5.9% moisture content and at both 15ºC/45%RH and 20ºC/55% RH conditions, independently of the package type used, showed the best results. Thus, macaw palm seeds can be classified as intermediates seeds.

  6. Lipids Characterization and Industrial Potentials of Pumpkin Seeds (Telfairia occidentalis and Cashew Nuts (Anacardium occidentale

    Directory of Open Access Journals (Sweden)

    E. O. Eddy

    2011-01-01

    Full Text Available Oil from Telfaria occidentalis and Anarcardium occidentale has been extracted and characterized. The lipid content of the Telfaria occidentalis and Anarcardium occidentalis were 58.41% and 42.15% respectively. The physicochemical parameters of Telfaria occidentalis and Anarcardium occidentale seeds were; boiling point; (58.90, 62.60 °C, melting point; (18.50, 21.80 °C, refractive index; (1.462, 1.498, specific gravity; (0.87, 0.69 saponification value; (91.16, 92.57 iodine value; (51.52, 47.20, acid value; (0.76, 3.74 ester value; (90.40, 88.87, % free fatty acid; (.38, 1.88 and peroxide value; (11.75, 15.23 respectively. Oils from these seeds were found to exhibit the needed potentials for utilization in paint and food industries and as biofuel.

  7. Effects of aqueous and ethanolic extracts of Peganum harmala L seeds on lipids profile in rats

    Directory of Open Access Journals (Sweden)

    Mohammad Mehdi Hasanzadeh Tahery

    2013-08-01

    Full Text Available Background and Aim: Atherosclerosis is the main factor of mortality in cardiovascular patients. Pathogenesis dependence of atherosclerosis on the increase of low density lipoprotein cholesterol (LDL-c has been proved. Since using of existing chemical medicines for the treatment of cardiovascular diseases causes several side-effects, this has led to more tendency to using of herbs.   According to some traditional medicinal references, Harmala seeds can be used as lowering blood lipids agents. The aim of the present study was to determine the effects of aqueous and ethanolic extracts of Harmala seeds on blood lipid profiles.   Materials and Methods: In this experimental study 18 Wistar rats with the average weight of 300 g were used. The rats were randomly divided into three groups, i.e. two experimental and one control group. The experimental groups I and II were respectively gavaged 3cc liquid containing 100mg/kg body weight (bw of aqueous and ethanolic extract of Harmala seeds for 30 days and group III, as a negative control, received an equal volume of distilled water for the same period. At the end , after deep anesthesia of the subjects with ether, the amount of blood lipids such as triglyceride (TG, total cholesterol (TC, LDL, and HDL were measured using test kits and the amount of VLDL cholesterol, too, was mathematically calculated.   The obtained data was analyzed by means of SPSS software (v: 11.5, ANOVA and Tukey tests at the significant level P<0.05.   Results: The findings showed that mean levels of LDL cholesterol in groups I, II, and control was 36.8±4.3, 35.8±4.3 and 44.3±4.6, respectively. Comparatively, the difference between experimental group I and the control (P=0.025 and also between group II and the control (P=0.012 were statistically significant. The other tested blood lipids in the experimental groups and the control group did not show a significant difference.   Conclusion: It was found that consumption of

  8. The extraction of total lipids from parsley: Petroselinum crispum (mill. Nym. Ex. A.W. Hill seeds

    Directory of Open Access Journals (Sweden)

    Stanković Mihajlo Z.

    2004-01-01

    Full Text Available The kinetics of extraction of total lipids from ground parsley (Petroselinum crispum (Mill. Nym. ex. A.W. Hill seeds with a mixture of ethanol or methanol with non-polar organic solvents, chloroform, carbon tetrachloride, trichloroethylene and petroleum ether, at various temperatures were studied. The maceration technique with reflux was used. The kinetic parameters were determined in extraction kinetic equations, as well as the optimal operation conditions for total lipids extraction. The maximum total lipids yield under optimal conditions was 33.7 g per 100 g of dry parsley seeds. Nine lipid fractions of the total lipids were separated by thin layer chromatography among which were phospholipids, sterol, mono-, di- and triacylglycerol, free fatty acids and carbohydrates.

  9. Absence of storage effects on radiation damage after thermal neutron irradiation of dry rice seeds

    Energy Technology Data Exchange (ETDEWEB)

    Kowyama, Y. [Mie Univ., Tsu (Japan); Saito, M.; Kawase, T.

    1987-09-15

    Storage effects on dry rice seeds equilibrated to 6.8% moisture content were examined after irradiation with X-rays of 5, 10, 20 and 40 kR and with thermal neutrons of 2.1, 4.2, 6.3 and 8.4×10{sup 13}N{sub th}/cm{sup 2}. Reduction in root growth was estimated from dose response curves after storage periods of 1 hr to 21 days. The longer the storage period, the greater enhancement of radiation damages in X-irradiated seeds. There were two components in the storage effect, i. e., a rapid increase of radiosensitivity within the first 24 hr and a slow increase up to 21 days. An almost complete absence of a storage effect was observed after thermal neutron exposure, in spite of considerably high radioactivities of the induced nuclides, {sup 56}Mn, {sup 42}K and {sup 24}Na, which were detected from gamma-ray spectrometry of the irradiated seeds. The present results suggest that the contributions of gamma-rays from the activated nuclides and of inherent contaminating gamma-rays are little or negligible against the neutron-induced damage, and that the main radiobiological effects of thermal neutrons are ascribed to in situ radiations, i, e., heavy particles resulting from neutron-capture reaction of atom. A mechanism underlying the absence of storage effect after thermal neutron irradiation was briefly discussed on the basis of radical formation and decay. (author)

  10. Lipid storage myopathy with clinical markers of Marfan syndrome: A rare association

    Directory of Open Access Journals (Sweden)

    Subasree Ramakrishnan

    2012-01-01

    Full Text Available Disorders of lipid metabolism can cause variable clinical presentations, often involving skeletal muscle, alone or together with other tissues. A 19-year-old boy presented with a 2-year history of muscle pain, cramps, exercise intolerance and progressive weakness of proximal lower limbs. Examination revealed skeletal markers of Marfan syndrome in the form of increased arm span compared with height, Kyphoscoliois, moderate pectus excavatum, high arched palate and wrist sign. He also had mild neck flexor weakness and proximal lower limb weakness with areflexia. Pathologic findings revealed lipid-laden fine vacuoles in the muscle fibers. Possibility of carnitine deficiency myopathy was considered and the patient was started on carnitine and Co Q. The patient made remarkable clinical improvement over the next 2 months. This case is reported for rarity of the association of clinical markers of Marfan syndrome and lipid storage myopathy and sparse literature on lipid storage myopathy in the Indian context.

  11. Fulminant lipid storage myopathy due to multiple acyl-coenzyme a dehydrogenase deficiency.

    Science.gov (United States)

    Whitaker, Charles H; Felice, Kevin J; Silvers, David; Wu, Qian

    2015-08-01

    The lipid storage myopathies, primary carnitine deficiency, neutral lipid storage disease, and multiple acyl coenzyme A dehydrogenase deficiency (MADD), are progressive disorders that cause permanent weakness. These disorders of fatty acid metabolism and intracellular triglyceride degradation cause marked fat deposition and damage to muscle cells. We describe a rapidly progressive myopathy in a previously healthy 33-year-old woman. Over 4 months, she developed a proximal and axial myopathy associated with diffuse myalgia and dysphagia, ultimately leading to respiratory failure and death. Muscle biopsy showed massive accumulation of lipid. Plasma acylcarnitine and urine organic acid analysis was consistent with MADD. This was confirmed by molecular genetic testing, which revealed 2 pathogenic mutations in the ETFDH gene. This report illustrates a late-onset case of MADD and reviews the differential diagnosis and evaluation of patients with proximal myopathy and excessive accumulation of lipid on muscle biopsy. © 2014 Wiley Periodicals, Inc.

  12. Sensitivity of Canola Seeds Associated Fungi to Gamma Rays During Storage

    International Nuclear Information System (INIS)

    Botros, H.W.

    2011-01-01

    The present study was carried out to investigate the possibility of using the gamma radiation to elongate the storage periods of canola seeds (Brassica naps L.). In this respect, canola seeds were irradiated at doses of 0.5, 1.5, 2.5, 3.5, 5.0 and 7.5 kGy gamma rays and stored at room temperature for periods 0, 3, 6, 9 and 12 months. The isolated fungi from non-irradiated post-harvest canola seeds included different species identified as Aspergillus flavus, A. niger, A. condidus, A. fumigatus, A. ochraceus, A. parasiticus, Fusarium oxysporium, F. moniliforme, Penicillium expansum, P. crysogenum, Alternaria brassicae, A. raphani and Trichoderma spp. It was noticed that the predominant species were A. ochraceus, A. flavus, A. niger and F. oxysporium at percentages 16.18, 14.73, 11.00 and 10.53%, respectively. The effective gamma irradiation on the predominant fungi (the sub-lethal dose) was 3.5 kGy for A. ochraceus and 5.0 kGy for F. oxysporium and F. moniliforme. Increasing the irradiated dose up to 7.5 kGy decreased significantly the growth of most isolated fungi. The data also showed that there was a decrease in the total fungal count in stored seeds under the effect of gamma rays for 12 months storage. Also, mycotoxins at the stored seeds were not detected after 12 months storage

  13. Effect of storage of shelled Moringa oleifera seeds from reaping time on turbidity removal.

    Science.gov (United States)

    Golestanbagh, M; Ahamad, I S; Idris, A; Yunus, R

    2011-09-01

    Moringa oleifera is an indigenous plant to Malaysia whose seeds are used for water purification. Many studies on Moringa oleifera have shown that it is highly effective as a natural coagulant for turbidity removal. In this study, two different methods for extraction of Moringa's active ingredient were investigated. Results of sodium chloride (NaCl) and distilled water extraction of Moringa oleifera seeds showed that salt solution extraction was more efficient than distilled water in extracting Moringa's active coagulant ingredient. The optimum dosage of shelled Moringa oleifera seeds extracted by the NaCl solution was comparable with that of the conventional chemical coagulant alum. Moreover, the turbidity removal efficiency was investigated for shelled Moringa oleifera seeds before drying in the oven under different storage conditions (i.e. open and closed containers at room temperature, 27 °C) and durations (fresh, and storage for 2, 4, 6 and 8 weeks from the time the seeds were picked from the trees). Our results indicate that there are no significant differences in coagulation efficiencies and, accordingly, turbidity removals between the examined storage conditions and periods.

  14. The technology of storage of a geno-fund of seeds of plants and animals

    International Nuclear Information System (INIS)

    Ombayev, A.M.; Tokhanov, M.T.; Burtebayeva, D.T.; Burtebayev, N.

    2002-01-01

    Because of an absent of special storages a geno-fund of plants seeds are stored mainly in usual unpractical laboratory conditions (in paper packs, in fabric bags, in metal boxes and so on). And during the first year of the storage the many Kinds of seeds begin to reduce a capacity of an inter-growth, and after several years they became to be useless to a sowing. As result, plant - breeders are forced to carry out frequent transplanting of collection samples, but it is connected with both significant material expenditures and a possible loss of a valuable geno-fund of seeds. But, from other hand, it is well known, that for a creation of only one new breed of animals it is necessary to carry out some experiments of during with from 15 to 30 years. With some economical reforms in Kazakhstan high - productive breeds of animals are on the brink of a disappearance. In connection with this the problem of the creation of the storage for the geno-fund of the seeds of the plants and of high - productive breeds of animals in Kazakhstan begins being an actual one. National storages, created in USA, Japan, Italy, Turkey, countries of SIS, correspond to definite standards, where there are maintained strict parameters for the storage active ventilation, storage in cooling state, a storage without of an access for air, a freezing in liquid nitrogen and in other coolants). Our investigations has established, that the storage of seeds of plants in gaseous medium with below-average content of oxygen increases a germinating power of seed. F.e. seeds of arid food plant of 'izen, Kochia prostrata', by natural way, keep the germinating power of seed, during about (6-8) months, and in gaseous medium its germinating power of seed reaches up to 5.5 years. Besides, at maturity of micro -organisms, there ore exterminated insects- pests and rodents. In order to improve mobility and a frost resistance of sperms of high productive stud-rams we had investigated the influence of electromagnetic

  15. Incidence of fungus and physiological quality of seeds of Jatropha curcas L. after cryogenic storage

    Directory of Open Access Journals (Sweden)

    Míriam Goldfarb

    2010-03-01

    Full Text Available The objective of this work was to evaluate the incidence of fungi in stored seeds of Jatropha curcas. The research was carried out at Cryogenic/UFCG, Sanity/UFPB and Cotton/Embrapa. The material for analysis showed an 8% water level, and 200 seeds were stored for treatment in cryogenic containers with nitrogen in the vapor and liquid phases. Four periods of crioconservation (0, 30, 60 and 90 days, were employed. After each period, the seeds were tested for sanity (Blotter test and germination. Superficial disinfestation, was carried out and seeds were distributed in Petri dishes, for incubation at 25 ± 2ºC, over a period of 7 days. The evaluation of the incidence of fungi was carried out in a stereoscopic microscope with observation of fungal structures, and values were expressed as percentages of seeds with fungus. The statistical experiment was completely randomized with temperature x days of storage. Analysis of variance was conducted and the means were compared by Tukey’s test at 5%. After 30 days of cryogenic storage, a greater incidence of Aspergillus sp., Cladosporium sp. and Fusarium sp. was detected. It was concluded that crioconservation at cryogenic temperatures did not reduce the incidence of fungus on Jatropha curcas seeds. The physiological quality was preserved during the cryoconservation.

  16. Effects of temperature during frozen storage on lipid deterioration of saithe (Pollachius virens) and hoki (Macruronus novaezelandiae) muscles.

    Science.gov (United States)

    Karlsdottir, Magnea G; Sveinsdottir, Kolbrun; Kristinsson, Hordur G; Villot, Dominique; Craft, Brian D; Arason, Sigurjon

    2014-08-01

    Lipid deterioration of two lean fish species, saithe (Pollachius virens) and hoki (Macruronus novaezelandiae), during frozen storage at -20 and -30°C (up to 18months) was studied. Lipid composition, lipid oxidation and hydrolysis, and sensory attributes were evaluated on both light and dark muscles of the fish species. Results showed significant lipid deterioration with extended storage time, but lower storage temperature showed significantly more preservative effects. A marked difference was observed between the composition of dark muscle of hoki and saithe. Polyunsaturated fatty acids were the predominant lipids in dark muscle of saithe, while monounsaturated fatty acids were predominant in dark muscle of hoki. Further, the hydrolytic activity differed greatly between dark muscle of hoki and saithe, with significantly lower activity observed in hoki. Present results indicate that both tertiary lipid oxidation and hydrolysis products are appropriate for assessing lipid deterioration of saithe and hoki light muscle during frozen storage. Copyright © 2014 Elsevier Ltd. All rights reserved.

  17. Mitochondrial dysfunction in human skeletal muscle biopsies of lipid storage disorder.

    Science.gov (United States)

    Debashree, Bandopadhyay; Kumar, Manish; Keshava Prasad, Thottethodi Subrahmanya; Natarajan, Archana; Christopher, Rita; Nalini, Atchayaram; Bindu, Parayil Sankaran; Gayathri, Narayanappa; Srinivas Bharath, Muchukunte Mukunda

    2018-02-09

    Mitochondria regulate the balance between lipid metabolism and storage in the skeletal muscle. Altered lipid transport, metabolism and storage influence the bioenergetics, redox status and insulin signalling, contributing to cardiac and neurological diseases. Lipid storage disorders (LSDs) are neurological disorders which entail intramuscular lipid accumulation and impaired mitochondrial bioenergetics in the skeletal muscle causing progressive myopathy with muscle weakness. However, the mitochondrial changes including molecular events associated with impaired lipid storage have not been completely understood in the human skeletal muscle. We carried out morphological and biochemical analysis of mitochondrial function in muscle biopsies of human subjects with LSDs (n = 7), compared to controls (n = 10). Routine histology, enzyme histochemistry and ultrastructural analysis indicated altered muscle cell morphology and mitochondrial structure. Protein profiling of the muscle mitochondria from LSD samples (n = 5) (vs. control, n = 5) by high-throughput mass spectrometric analysis revealed that impaired metabolic processes could contribute to mitochondrial dysfunction and ensuing myopathy in LSDs. We propose that impaired fatty acid and respiratory metabolism along with increased membrane permeability, elevated lipolysis and altered cristae entail mitochondrial dysfunction in LSDs. Some of these mechanisms were unique to LSD apart from others that were common to dystrophic and inflammatory muscle pathologies. Many differentially regulated mitochondrial proteins in LSD are linked with other human diseases, indicating that mitochondrial protection via targeted drugs could be a treatment modality in LSD and related metabolic diseases. © 2018 International Society for Neurochemistry.

  18. Effect of liquid nitrogen storage on seed germination of 51 tree species

    Science.gov (United States)

    Jill R. Barbour; Bernard R. Parresol

    2003-01-01

    Two liquid nitrogen storage experiments were performed on 51 tree species. In experiment 1, seeds of 9western tree species were placed in a liquid nitrogen tank for 3 time periods: 24 hours, 4 weeks, and 222 days. A corresponding control sample accompanied each treatment. For three species,Calocedrus decurrens, Pinus jefferyi, and ...

  19. Structure Annotation and Quantification of Wheat Seed Oxidized Lipids by High-Resolution LC-MS/MS.

    Science.gov (United States)

    Riewe, David; Wiebach, Janine; Altmann, Thomas

    2017-10-01

    Lipid oxidation is a process ubiquitous in life, but the direct and comprehensive analysis of oxidized lipids has been limited by available analytical methods. We applied high-resolution liquid chromatography-mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) to quantify oxidized lipids (glycerides, fatty acids, phospholipids, lysophospholipids, and galactolipids) and implemented a platform-independent high-throughput-amenable analysis pipeline for the high-confidence annotation and acyl composition analysis of oxidized lipids. Lipid contents of 90 different naturally aged wheat ( Triticum aestivum ) seed stocks were quantified in an untargeted high-resolution LC-MS experiment, resulting in 18,556 quantitative mass-to-charge ratio features. In a posthoc liquid chromatography-tandem mass spectrometry experiment, high-resolution MS/MS spectra (5 mD accuracy) were recorded for 8,957 out of 12,080 putatively monoisotopic features of the LC-MS data set. A total of 353 nonoxidized and 559 oxidized lipids with up to four additional oxygen atoms were annotated based on the accurate mass recordings (1.5 ppm tolerance) of the LC-MS data set and filtering procedures. MS/MS spectra available for 828 of these annotations were analyzed by translating experimentally known fragmentation rules of lipids into the fragmentation of oxidized lipids. This led to the identification of 259 nonoxidized and 365 oxidized lipids by both accurate mass and MS/MS spectra and to the determination of acyl compositions for 221 nonoxidized and 295 oxidized lipids. Analysis of 15-year aged wheat seeds revealed increased lipid oxidation and hydrolysis in seeds stored in ambient versus cold conditions. © 2017 The author(s). All Rights Reserved.

  20. Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius

    Directory of Open Access Journals (Sweden)

    Goggin Danica E

    2011-04-01

    Full Text Available Abstract Background In legumes, seed storage proteins are important for the developing seedling and are an important source of protein for humans and animals. Lupinus angustifolius (L., also known as narrow-leaf lupin (NLL is a grain legume crop that is gaining recognition as a potential human health food as the grain is high in protein and dietary fibre, gluten-free and low in fat and starch. Results Genes encoding the seed storage proteins of NLL were characterised by sequencing cDNA clones derived from developing seeds. Four families of seed storage proteins were identified and comprised three unique α, seven β, two γ and four δ conglutins. This study added eleven new expressed storage protein genes for the species. A comparison of the deduced amino acid sequences of NLL conglutins with those available for the storage proteins of Lupinus albus (L., Pisum sativum (L., Medicago truncatula (L., Arachis hypogaea (L. and Glycine max (L. permitted the analysis of a phylogenetic relationships between proteins and demonstrated, in general, that the strongest conservation occurred within species. In the case of 7S globulin (β conglutins and 2S sulphur-rich albumin (δ conglutins, the analysis suggests that gene duplication occurred after legume speciation. This contrasted with 11S globulin (α conglutin and basic 7S (γ conglutin sequences where some of these sequences appear to have diverged prior to speciation. The most abundant NLL conglutin family was β (56%, followed by α (24%, δ (15% and γ (6% and the transcript levels of these genes increased 103 to 106 fold during seed development. We used the 16 NLL conglutin sequences identified here to determine that for individuals specifically allergic to lupin, all seven members of the β conglutin family were potential allergens. Conclusion This study has characterised 16 seed storage protein genes in NLL including 11 newly-identified members. It has helped lay the foundation for efforts to use

  1. Evaluating the safety of phytosterols removed perilla seed oil-based lipid emulsion.

    Science.gov (United States)

    Yang, Zhenhua; Ren, Tianyang; Lu, De; Guo, Haiyan; Li, Wanqiu; Huang, Chenglong; He, Haibing; Liu, Dongchun; Tang, Xing

    2016-10-01

    The aim of this study was to ascertain the potential toxicity of perilla seed oil-based lipid emulsion (POLE) caused by phytosterols and confirm the efficacy of the technique for removing phytosterols from perilla seed oil, and evaluate the safety of a low phytosterol POLE in a long-term tolerance study in dogs. A comparison between a soybean oil lipid emulsion (Intralipid group A) and POLE with high (group B) versus low (group C) levels of phytosterols was made with regard to their effects on the general condition, hematological and biochemical parameters, urinalysis and histopathological changes in nine dogs receiving daily infusions for four weeks at dosage levels of 6, 6, 9 g fat /kg. Dogs in group A and group C remained in good condition and gained weight during the infusion period and no diarrhea or gastrointestinal bleeding occurred. Only a moderate degree of anemia was observed, the biochemical parameters changed only slightly and returned to normal after treatment had ceased. However, the dogs in group B exhibited significant symptoms of 'fat overload syndrome'. Vomiting, diarrhoea and blood in the faeces were observed. Moreover, triglyceridemia, cholesteremia, and dark urine as well as microscopic signs of liver and gastrointestinal tract damage and generalized jaundice were clearly seen. Phytosterols promote 'fat overload syndrome' in long-term tolerance studies of POLE in dogs by producing cholestatic liver injury and interfering with fat metabolism. And the toxicity of POLE was reduced by removing phytosterols.

  2. Chemical composition of Sacha Inchi (Plukenetia volubilis L.) seeds and characteristics of their lipid fraction

    Energy Technology Data Exchange (ETDEWEB)

    Gutierrrez, L.F.; Rosada, L.M.; Jimenez, A.

    2011-07-01

    The seeds of Sacha inchi (Plukenetia volubilis L.) from Colombia were analyzed for their main chemical composition. Sacha inchi seeds (SIS) were rich in oil (41.4%) and protein (24.7%). The main minerals present in SIS were potassium (5563.5 ppm), magnesium (3210 ppm) and calcium (2406 ppm). A fatty acid analysis revealed that a-linolenic (50.8%) and linoleic (33.4%) acids were the main fatty acids in Sacha inchi oil (SIO). The lipid fractionation of SIO, obtained by solid phase extraction, yielded mainly neutral lipids (97.2%), and lower amounts of free fatty acids (1.2%) and phospholipids (0.8%). The physicochemical properties of the oil include: saponification number 185.2; iodine value 193.1; density 0.9187 g/cm3, refractive index 0.4791 and viscosity of 35.4 mPa.s. The melting profiles of SIO were characterized by the presence of one wide endothermic peak with a melting enthalpy of 23.2 J/g. Our results indicate that Sacha inchi is an important new crop with applications in the food and pharmaceutical industries. (Author).

  3. Storage stability of cauliflower soup powder: The effect of lipid oxidation and protein degradation reactions.

    Science.gov (United States)

    Raitio, Riikka; Orlien, Vibeke; Skibsted, Leif H

    2011-09-15

    Soups based on cauliflower soup powders, prepared by dry mixing of ingredients and rapeseed oil, showed a decrease in quality, as evaluated by a sensory panel, during the storage of the soup powder in the dark for up to 12weeks under mildly accelerated conditions of 40°C and 75% relative humidity. Antioxidant, shown to be effective in protecting the rapeseed bulk oil, used for the powder preparation, had no effect on storage stability of the soup powder. The freshly prepared soup powder had a relatively high concentration of free radicals, as measured by electron spin resonance spectroscopy, which decreased during storage, and most remarkably during the first two weeks of storage, with only marginal increase in lipid hydroperoxides as primary lipid oxidation products, and without any increase in secondary lipid oxidation products. Analyses of volatiles by SPME-GC-MS revealed a significant increase in concentrations of 2-methyl- and 3-methyl butanals, related to Maillard reactions, together with an increase in 2-acetylpyrrole concentration. The soup powders became more brown during storage, as indicated by a decreasing Hunter L-value, in accord with non-enzymatic browning reactions. A significant increase in the concentrations of dimethyl disulfide in soup powder headspace indicated free radical-initiated protein oxidation. Protein degradation, including Maillard reactions and protein oxidation, is concluded to be more important than lipid oxidation in determining the shelf-life of dry cauliflower soup powder. Copyright © 2011 Elsevier Ltd. All rights reserved.

  4. Oxidative stability during storage of structured lipids produced from fish oil and caprylic acid

    DEFF Research Database (Denmark)

    Nielsen, Nina Skall; Xu, Xuebing; Timm Heinrich, Maike

    2004-01-01

    Structured lipids produced by enzymatic or chemical methods for different applications have been receiving considerable attention. The oxidative stability of a randomized structured lipid (RFO), produced by chemical interesterification from fish oil (FO) and tricaprylin, and a specific structured...... lipid (SFO), produced by enzymatic interesterification from the same oil and caprylic acid, was compared with the stability of FO. Oils were stored at 2degreesC for 11 wk followed by storage at 20degreesC for 6 wk. In addition, the antioxidative effect of adding the metal chelators EDTA or citric acid...

  5. Effect of cold storage, heat, smoke and charcoal on breaking seed dormancy of Arctostaphylos pungens HBK (Ericaceae)

    OpenAIRE

    Jurado, E; Márquez-Linares, M; Flores, J

    2011-01-01

    We evaluated the effect of cold storage and fire-related cues on seed germination of Arctostaphylos pungens HBK (Mexican Manzanita), a common shrub in poorly managed pine-oak forests in Durango, Mexico. Because this shrub has a high density in previously burnt forests, we investigated the effect that high temperatures, smoke and charcoal might have on seed germination of this species. Seeds were collected fresh from the shrubs. The highest germination was 30% for seeds that had been cold stor...

  6. Lupin seeds lower plasma lipid concentrations and normalize antioxidant parameters in rats

    Directory of Open Access Journals (Sweden)

    Osman, M.

    2011-06-01

    Full Text Available This study was designed to test bitter and sweet lupin seeds for lipid-lowering and for their antioxidative activities in hypercholesterolemic rats. The levels of plasma lipid, malondialdehyde (MDA and whole blood reduced glutathione (GSH, as well as the activities of transaminases (ALT and AST, lactate dehydrogenase (LDH in plasma, superoxide dismutase (SOD, glutathione peroxidase (GPx in erythrocytes and plasma glutathione reductase (GR, glutathione-S-transferase (GST and catalase (CAT were examined. A hypercholesterolemia-induced diet manifested in the elevation of total lipids (TL, total cholesterol (TC, triglycerides (TG, LDL-C and MDA levels, ALT, AST, LDH activities and the depletion of GSH and enzymic antioxidants. The supplementation of a hypercholesterolemia-induced diet with bitter and sweet lupin seeds significantly lowered the plasma levels of TL, TC, TG and LDL-C. ALT, AST and LDH activities slightly decreased in treated groups compared with the hypercholesterolemic group (HC. Furthermore, the content of GSH significantly increased while MDA significantly decreased in treated groups compared with the HC group. In addition, the bitter lupin seed group improved enzymic antioxidants compared with the HC group. In general, the results indicated that the bitter lupin seed supplements are better than those containing sweet lupin seeds. These results suggested that the hypocholesterolemic effect of bitter and sweet lupin seed supplements might be due to their abilities to lower the plasma cholesterol level as well as to slow down the lipid peroxidation process and to enhance the antioxidant enzyme activity.

    Este estudio fue diseñado para evaluar semillas de altramuces dulces y amargas como agentes que bajan los lípidos y estudiar su efecto en la actividad antioxidante en ratas hipercolesterolémicas. El nivel de lípidos en plasma, malondialdehido (MDA y glutatión reducido (GSH, así como la actividad transaminasa (ALT y AST

  7. Antifungal Activity of Leaf and Latex Extracts of Calotropis procera (Ait.) against Dominant Seed-Borne Storage Fungi of Some Oil Seeds

    OpenAIRE

    Manoorkar V B; Mandge S V; B D Gachande

    2015-01-01

    In present study, aqueous and ethanol extracts of leaf & latex of Calotropis procera (Ait.) was tested for their antifungal activity against dominant storage seed-borne fungi of some oil seeds such as groundnut, soybean, sunflower and mustard. The antifungal effect of ethanol and aqueous extracts of leaf & latex of Calotropis procera (Ait.) against ten seed-borne dominant fungi viz., Cuvularia lunata, Alternaria alternata, Rhizoctonia solani, Fusarium solani, Penicillium chrysogenum, Asperg...

  8. Mature forms of the major seed storage albumins in sunflower: A mass spectrometric approach.

    Science.gov (United States)

    Franke, Bastian; Colgrave, Michelle L; Mylne, Joshua S; Rosengren, K Johan

    2016-09-16

    Seed storage albumins are abundant, water-soluble proteins that are degraded to provide critical nutrients for the germinating seedling. It has been established that the sunflower albumins encoded by SEED STORAGE ALBUMIN 2 (SESA2), SESA20 and SESA3 are the major components of the albumin-rich fraction of the common sunflower Helianthus annuus. To determine the structure of sunflowers most important albumins we performed a detailed chromatographic and mass spectrometric characterization to assess what post-translational processing they receive prior to deposition in the protein storage vacuole. We found that SESA2 and SESA20 each encode two albumins. The first of the two SESA2 albumins (SESA2-1) exists as a monomer of 116 or 117 residues, differing by a threonine at the C-terminus. The second of the two SESA2 albumins (SESA2-2) is a monomer of 128 residues. SESA20 encodes the albumin SESA20-2, which is a 127-residue monomer, whereas SESA20-1 was not abundant enough to be structurally described. SESA3, which has been partly characterized previously, was found in several forms with methylation of its asparagine residues. In contrast to other dicot albumins, which are generally matured into a heterodimer, all the dominant mature sunflower albumins SESA2, SESA20-2, SESA3 and its post-translationally modified analogue SESA3-a are monomeric. Sunflower plants have been bred to thrive in various climate zones making them favored crops to meet the growing worldwide demand by humans for protein. The abundance of seed storage proteins makes them an important source of protein for animal and human nutrition. This study explores the structures of the dominant sunflower napin-type seed storage albumins to understand what structures evolution has favored in the most abundant proteins in sunflower seed. Crown Copyright © 2016. Published by Elsevier B.V. All rights reserved.

  9. Influence of foliar fertilization with manganese on germination, vigor and storage time of RR soybean seeds

    Directory of Open Access Journals (Sweden)

    Vanessa Leonardo Ignácio

    2015-10-01

    Full Text Available ABSTRACTThis study aimed to evaluate the influence of foliar fertilizer doses containing Mn of phenological stages of suggested application in RR soybeans, to recover management damages with glyphosate at postemergence application on seed vigor in post-harvest and post six months storage. The seeds originated from a field experiment conducted , which included two applications of glyphosate, concomitant with foliar fertilizer in growth stages V4 and V6, with 0.00, 113.50 and 227.00 mg ha-1doses of Mn2+. Germination, GSI (Germination Speed Index, electrical conductivity tests and the first count of seeds were conducted. The application of Mn did not affect the physiological quality of RR soy in postharvest. However, in post-storage, higher doses of Mn had a negative effect on tests of abnormal seedlings, GSI and electrical conductivity. The applications of Mn, regardless of the developmental stage, did not interfere in the germination and first count tests, with and without storage. The electrical conductivity test showed a higher correlation with the seed germination test in the post-harvest treatment.

  10. Effect of pomegranate peel extract on lipid and protein oxidation in beef meatballs during refrigerated storage.

    Science.gov (United States)

    Turgut, Sebahattin Serhat; Soyer, Ayla; Işıkçı, Fatma

    2016-06-01

    Antioxidant effect of pomegranate peel extract (PE) to retard lipid and protein oxidation was investigated in meatballs during refrigerated storage at 4±1°C. Concentrated lyophilised water extract of pomegranate peel was incorporated into freshly minced beef meat at 0.5% and 1% concentrations and compared with 0.01% butylated hydroxytoluene (BHT) as a reference and control (without any antioxidant). PE showed high phenolic content and antioxidant activity. In PE added samples, thiobarbituric acid reactive substances (TBARS) value, peroxide formation, loss of sulfhydryl groups and formation of protein carbonyls were lower than control (Pmeatballs prolonged the refrigerated storage up to 8 days. Addition of both 0.5 and 1% PE in meatballs reduced lipid and protein oxidation and improved sensory scores. These results indicated that PE was effective on retarding lipid and protein oxidation. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. Polycyclic aromatic hydrocarbons storage by Fusarium solani in intracellular lipid vesicles

    International Nuclear Information System (INIS)

    Verdin, Anthony; Lounes-Hadj Sahraoui, Anissa; Newsam, Ray; Robinson, Gary; Durand, Roger

    2005-01-01

    Accumulation and elimination of polycyclic aromatic hydrocarbons (PAHs) were studied in the fungus Fusarium solani. When the fungus was grown on a synthetic medium containing benzo[a]pyrene, hyphae of F. solani contained numerous lipid vesicles which could be stained by the lipid-specific dyes: Sudan III and Rhodamine B. The fluorescence produced by Rhodamine B and PAH benzo[a]pyrene were at the same locations in the fungal hyphae, indicating that F. solani stored PAH in pre-existing lipid vesicles. A passive temperature-independent process is involved in the benzo[a]pyrene uptake and storage. Sodium azide, a cytochrome c oxidation inhibitor, and the two cytoskeleton inhibitors colchicine and cytochalasin did not prevent the transport and accumulation of PAH in lipid vesicles of F. solani hyphae. F. solani degraded a large range of PAHs at different rates. PAH intracellular storage in lipid vesicles was not necessarily accompanied by degradation and was common to numerous other fungi. - Fungi can store PAHs intracellularly in lipid vesicles independently of their PAH degradation abilities

  12. Polycyclic aromatic hydrocarbons storage by Fusarium solani in intracellular lipid vesicles

    Energy Technology Data Exchange (ETDEWEB)

    Verdin, Anthony [Laboratoire de Mycologie/Phytopathologie/Environnement, Universite du Littoral-Cote d' Opale, 17 avenue Bleriot, BP 699, 62228 Calais Cedex (France); Lounes-Hadj Sahraoui, Anissa [Laboratoire de Mycologie/Phytopathologie/Environnement, Universite du Littoral-Cote d' Opale, 17 avenue Bleriot, BP 699, 62228 Calais Cedex (France)]. E-mail: lounes@univ-littoral.fr; Newsam, Ray [Department of Biosciences, University of Kent, Canterbury CT2 7NJ (United Kingdom); Robinson, Gary [Department of Biosciences, University of Kent, Canterbury CT2 7NJ (United Kingdom); Durand, Roger [Laboratoire de Mycologie/Phytopathologie/Environnement, Universite du Littoral-Cote d' Opale, 17 avenue Bleriot, BP 699, 62228 Calais Cedex (France)

    2005-01-01

    Accumulation and elimination of polycyclic aromatic hydrocarbons (PAHs) were studied in the fungus Fusarium solani. When the fungus was grown on a synthetic medium containing benzo[a]pyrene, hyphae of F. solani contained numerous lipid vesicles which could be stained by the lipid-specific dyes: Sudan III and Rhodamine B. The fluorescence produced by Rhodamine B and PAH benzo[a]pyrene were at the same locations in the fungal hyphae, indicating that F. solani stored PAH in pre-existing lipid vesicles. A passive temperature-independent process is involved in the benzo[a]pyrene uptake and storage. Sodium azide, a cytochrome c oxidation inhibitor, and the two cytoskeleton inhibitors colchicine and cytochalasin did not prevent the transport and accumulation of PAH in lipid vesicles of F. solani hyphae. F. solani degraded a large range of PAHs at different rates. PAH intracellular storage in lipid vesicles was not necessarily accompanied by degradation and was common to numerous other fungi. - Fungi can store PAHs intracellularly in lipid vesicles independently of their PAH degradation abilities.

  13. Carp (Cyprinus carpio L. lipid oxidation during cold storage

    Directory of Open Access Journals (Sweden)

    Ochrem Andrzej S.

    2015-06-01

    Full Text Available After meat and dairy products, fish meat is one of the greatest sources of animal protein, but it is also probably the most susceptible to oxidation. The study material consisted of carp, Cyprinus carpio L., meat. The left and right sides of the fillet were divided into three parts, the head, abdomen, and tail. On the first day of the study, the anisidine value ranged from 0.21-1.70, and did not differ significantly among the body parts. Peroxide values fluctuated frequently during the study. Lipid oxidation depended on the part of the fish the meat came from and the structure of the meat, either fillets or ground meat.

  14. Effect of irradiation on lipid oxidation in eviscerated chicken carcasses during storage

    International Nuclear Information System (INIS)

    Hassan, I.M.; Hussein, M.F.; Mahmoud, A.A.; Hegazy, R.A.

    1988-01-01

    Oxidative changes induced in pectoralis major muscle of chicken after irradiation treatments with 0,6,10 and 20 KGy in both non frozen or frozen conditions during subsequent storage were investigated. Ultraviolet (UV) absorption, peroxides and thiobarbituric reactive substances increased in chicken lipids with increasing irradiation doses. These oxidative changes are greater in irradiated refrigerated (4 ± 1°C) than in irradiated frozen (-20°C) chicken lipids during storage. It was found the peroxides and TBA reactive substances do not accumulate as a stable end products of fat oxidation but reach a maximum during storage followed by gradual declining. The UV absorption provides an objective measure of chicken lipids autoxidation suitable for following the progress of autoxidation of irradiated chicken during subsequent non frozen (4 ± 1°C) or frozen (-20°C) storage. The extent of Maillard-like browning was followed in both unirradiated and irradiated samples during storage. All tested objective parameters correlated well with sensory assessment of odour particularly when irradiation dose was increased as well as in frozen samples

  15. Study of lipid profile and parieto-temporal lipid peroxidation in AlCl3 mediated neurotoxicity. modulatory effect of fenugreek seeds

    Directory of Open Access Journals (Sweden)

    Belaïd-Nouira Yosra

    2012-01-01

    Full Text Available Abstract Background Peroxidation of lipid (LPO membrane and cholesterol metabolism have been involved in the physiopathology of many diseases of aging brain. Therefore, this prospective animal study was carried firstly to find out the correlation between LPO in posterior brain and plasmatic cholesterol along with lipoprotein levels after chronic intoxication by aluminium chloride (AlCl3. Chronic aluminum-induced neurotoxicity has been in fact related to enhanced brain lipid peroxidation together with hypercholesterolemia and hypertriglyceridemia, despite its controversial etiological role in neurodegenerative diseases. Secondly an evaluation of the effectiveness of fenugreek seeds in alleviating the engendered toxicity through these biochemical parameters was made. Results Oral administration of AlCl3 to rats during 5 months (500 mg/kg bw i.g for one month then 1600 ppm via the drinking water enhanced the levels of LPO in posterior brain, liver and plasma together with lactate dehydrogenase (LDH activities, total cholesterol (TC, triglycerides (TG and LDL-C (Low Density Lipoproteins levels. All these parameters were decreased following fenugreek seeds supplementation either as fenugreek seed powder (FSP or fenugreek seed extract (FSE. A notable significant correlation was observed between LPObrain and LDL-C on one hand and LDHliver on the other hand. This latter was found to correlate positively with TC, TG and LDL-C. Furthermore, high significant correlations were observed between LDHbrain and TC, TG, LDL-C, LPObrain as well as LDHliver. Conclusion Aluminium-induced LPO in brain could arise from alteration of lipid metabolism particularly altered lipoprotein metabolism rather than a direct effect of cholesterol oxidation. Fenugreek seeds could play an anti-peroxidative role in brain which may be attributed in part to its modulatory effect on plasmatic lipid metabolism.

  16. Single Cell Synchrotron FT-IR Microspectroscopy Reveals a Link between Neutral Lipid and Storage Carbohydrate Fluxes in S. cerevisiae

    Science.gov (United States)

    Jamme, Frédéric; Vindigni, Jean-David; Méchin, Valérie; Cherifi, Tamazight; Chardot, Thierry; Froissard, Marine

    2013-01-01

    In most organisms, storage lipids are packaged into specialized structures called lipid droplets. These contain a core of neutral lipids surrounded by a monolayer of phospholipids, and various proteins which vary depending on the species. Hydrophobic structural proteins stabilize the interface between the lipid core and aqueous cellular environment (perilipin family of proteins, apolipoproteins, oleosins). We developed a genetic approach using heterologous expression in Saccharomyces cerevisiae of the Arabidopsis thaliana lipid droplet oleosin and caleosin proteins AtOle1 and AtClo1. These transformed yeasts overaccumulate lipid droplets, leading to a specific increase in storage lipids. The phenotype of these cells was explored using synchrotron FT-IR microspectroscopy to investigate the dynamics of lipid storage and cellular carbon fluxes reflected as changes in spectral fingerprints. Multivariate statistical analysis of the data showed a clear effect on storage carbohydrates and more specifically, a decrease in glycogen in our modified strains. These observations were confirmed by biochemical quantification of the storage carbohydrates glycogen and trehalose. Our results demonstrate that neutral lipid and storage carbohydrate fluxes are tightly connected and co-regulated. PMID:24040242

  17. Single cell synchrotron FT-IR microspectroscopy reveals a link between neutral lipid and storage carbohydrate fluxes in S. cerevisiae.

    Directory of Open Access Journals (Sweden)

    Frédéric Jamme

    Full Text Available In most organisms, storage lipids are packaged into specialized structures called lipid droplets. These contain a core of neutral lipids surrounded by a monolayer of phospholipids, and various proteins which vary depending on the species. Hydrophobic structural proteins stabilize the interface between the lipid core and aqueous cellular environment (perilipin family of proteins, apolipoproteins, oleosins. We developed a genetic approach using heterologous expression in Saccharomyces cerevisiae of the Arabidopsis thaliana lipid droplet oleosin and caleosin proteins AtOle1 and AtClo1. These transformed yeasts overaccumulate lipid droplets, leading to a specific increase in storage lipids. The phenotype of these cells was explored using synchrotron FT-IR microspectroscopy to investigate the dynamics of lipid storage and cellular carbon fluxes reflected as changes in spectral fingerprints. Multivariate statistical analysis of the data showed a clear effect on storage carbohydrates and more specifically, a decrease in glycogen in our modified strains. These observations were confirmed by biochemical quantification of the storage carbohydrates glycogen and trehalose. Our results demonstrate that neutral lipid and storage carbohydrate fluxes are tightly connected and co-regulated.

  18. Lipid Involvement in Neurodegenerative Diseases of the Motor System: Insights from Lysosomal Storage Diseases.

    Science.gov (United States)

    Dodge, James C

    2017-01-01

    Lysosomal storage diseases (LSDs) are a heterogeneous group of rare inherited metabolic diseases that are frequently triggered by the accumulation of lipids inside organelles of the endosomal-autophagic-lysosomal system (EALS). There is now a growing realization that disrupted lysosomal homeostasis (i.e., lysosomal cacostasis) also contributes to more common neurodegenerative disorders such as Parkinson disease (PD). Lipid deposition within the EALS may also participate in the pathogenesis of some additional neurodegenerative diseases of the motor system. Here, I will highlight the lipid abnormalities and clinical manifestations that are common to LSDs and several diseases of the motor system, including amyotrophic lateral sclerosis (ALS), atypical forms of spinal muscular atrophy, Charcot-Marie-Tooth disease (CMT), hereditary spastic paraplegia (HSP), multiple system atrophy (MSA), PD and spinocerebellar ataxia (SCA). Elucidating the underlying basis of intracellular lipid mislocalization as well as its consequences in each of these disorders will likely provide innovative targets for therapeutic research.

  19. Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry

    Directory of Open Access Journals (Sweden)

    Pramod Kumar Singh

    2016-01-01

    Full Text Available Proteomic analysis was employed to map the seed storage protein network in landrace and cultivated chickpea accessions. Protein extracts were separated by two-dimensional gel electrophoresis (2D-GE across a broad range 3.0–10.0 immobilized pH gradient (IPG strips. Comparative elucidation of differentially expressed proteins between two diverse geographically originated chickpea accessions was carried out using 2D-GE coupled with mass spectrometry. A total of 600 protein spots were detected in these accessions. In-gel protein expression patterns revealed three protein spots as upregulated and three other as downregulated. Using trypsin in-gel digestion, these differentially expressed proteins were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS which showed 45% amino acid homology of chickpea seed storage proteins with Arabidopsis thaliana.

  20. Seed-storage Mycoflora of Peanut Cultivars Grown in Nigerian Savanna

    Directory of Open Access Journals (Sweden)

    Nwankiti, AO.

    2003-01-01

    Full Text Available Storage of peanut is increasingly becoming important both among growers and users of the crop in Nigerian savanna. The aim is to sell the produce and maximize benefits accruing from the crops during scarcity. Very often, these envisaged advantages fail due to unfavourable market forces, thus compelling them to sell at a loss or store them across seasons for periods ranging from one to two years. However, information on fungi associated with storage of such peanuts in Nigerian savanna and its attendant problems are yet to be investigated. Thus, the seed mycoflora and viability seven common peanut cultivars stored under conditions similar to traditional settings were investigated using different isolating techniques. The peanut cultivars were RMP 12, RMP 91, RRB, 48-115B, M554-76, 55-437 Ex-Dakar and a local cultivar. None of these cultivars possessed resistance to in vitro colonization by fungi. Aspergillus niger, Aspergillus flavus and Rhizopus stolonifer were consistently isolated from all the cultivars from almost all isolating techniques. Other fungi were Fusarium chlamydosporium, F. roseum, F. oxysporium, Penicillium spp., Curvularia spp., Botryodiplodia theobromae, Macrophomina phaseolina and Sclerotium rolfsii. Relative percentages, however, varied with individual fungi and peanut cultivars. The test with seven different types of growth media gave the highest fungi recovery rate than the blotter paper technique. Seed viability was lower with peanut seeds stored for two years. Also, the relative percentage occurrence of individual fungi was significantly higher with seeds stored for two years. While we recommend the use of growth media for recovery and study of seed mycoflora, peanut seeds should not be stored for more than one year.

  1. Characterization of Seed Storage Proteins from Chickpea Using 2D Electrophoresis Coupled with Mass Spectrometry

    OpenAIRE

    Singh, Pramod Kumar; Shrivastava, Nidhi; Chaturvedi, Krishna; Sharma, Bechan; Bhagyawant, Sameer S.

    2016-01-01

    Proteomic analysis was employed to map the seed storage protein network in landrace and cultivated chickpea accessions. Protein extracts were separated by two-dimensional gel electrophoresis (2D-GE) across a broad range 3.0–10.0 immobilized pH gradient (IPG) strips. Comparative elucidation of differentially expressed proteins between two diverse geographically originated chickpea accessions was carried out using 2D-GE coupled with mass spectrometry. A total of 600 protein spots were detected ...

  2. Effects of gamma radiation and storage on cooked pine seed (Araucaria angustifollia)

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Lucia A.C.S.; Modolo, Debora M.; Martinez, Patricia; Piero, Edson A. di; Bigide, Priscila; Arthur, Valter, E-mail: lcasilva@cena.usp.br, E-mail: arthur@cena.usp.br [Centro de Energia Nuclear na Agricultura (CENA/USP), Laboratorio de Radiobiologia e Ambiente, Piracicaba, SP (Brazil); Harder, Marcia N.C.; Arthur, Paula B. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    The Araucaria angustifolia, is known as the Pinheiro-do-Parana Brazilian pine, Pine, Pine Tree Monkey, emerges as the main representative of the Rain Forest, also known as Araucaria Forest, part of the Atlantic Forest biome (Decree Law 750/1993). Despite being appreciated nutritious food, the gear has been widely used in Brazilian cook as other seeds, and its consumption in the more usual way roasted or boiled, however, certain foods have been developed, such as flour, pine seeds, artisan produced only due to poor commercial expression. Because of this, the aim of this work was to study the effect of storage under vacuum and gamma radiation on samples cooked pinion. Pine seeds after cooking were stored in vacuum packaging and polypropylene irradiated with 0 (control), 0.5, 1.0 and 3.0 kGy. Later they were stored at a temperature of 6 degree C. Analyzes were performed to characterize physical (weight, temperature, percentage of losses) and proximate composition (Humidity, fat, protein, ash and weight loss) of A. angustifolia (Bert.) pine seed after three months of storage. The results indicated that there was no significant difference between treatments at protein parameter. About the other parameters there was an increase humidity and decrease with ash and fat with the treatments. (author)

  3. Preparation and Low Temperature Short-term Storage for Synthetic Seeds of Caladium bicolor

    Directory of Open Access Journals (Sweden)

    Mehpara MAQSOOD

    2015-03-01

    Full Text Available An efficient somatic embryo encapsulation and in vitro plant regeneration technique were established with Caladium bicolor, an important ornamental plant.Tuber derived embryogenic callus (95.50% was obtained on Murashige and Skoog (MS medium amended with 0.5 mg L-1 α-Naphthalene acetic acid (NAA + 0.5 mg L-1 6-Benzyladenine (BA. The embryogenic callus later differentiated into somatic embryos in the same plant growth regulators (PGRs added medium (NAA and BA. The induced embryos matured and developed into plantlets in NAA and BA added media; maximum plantlets development was observed at 1.0 mg L-1 NAA + 1.0 mg L-1 BA supplemented medium. Synthetic seeds were produced by encapsulating embryos in gel containing 3.0% sucrose + 3.0% sodium alginate and 100 mM of calcium chloride.The highest synthetic seed germination (97.6% was observed on medium supplemented with 1.0 mg L-1 NAA + 1.0 mg L-1 BA. The synthetic seeds were kept at low temperatures for storage; the encapsulated beads were viable and demonstrated good germination even after 12 weeks of storage at 4 °C. The plantlet recovery frequency was however declined with time. The synthetic seed derived plantlets were morphologically similar to the mother plant.

  4. Effects of gamma radiation and storage on cooked pine seed (Araucaria angustifollia)

    International Nuclear Information System (INIS)

    Silva, Lucia A.C.S.; Modolo, Debora M.; Martinez, Patricia; Piero, Edson A. di; Bigide, Priscila; Arthur, Valter; Harder, Marcia N.C.; Arthur, Paula B.

    2011-01-01

    The Araucaria angustifolia, is known as the Pinheiro-do-Parana Brazilian pine, Pine, Pine Tree Monkey, emerges as the main representative of the Rain Forest, also known as Araucaria Forest, part of the Atlantic Forest biome (Decree Law 750/1993). Despite being appreciated nutritious food, the gear has been widely used in Brazilian cook as other seeds, and its consumption in the more usual way roasted or boiled, however, certain foods have been developed, such as flour, pine seeds, artisan produced only due to poor commercial expression. Because of this, the aim of this work was to study the effect of storage under vacuum and gamma radiation on samples cooked pinion. Pine seeds after cooking were stored in vacuum packaging and polypropylene irradiated with 0 (control), 0.5, 1.0 and 3.0 kGy. Later they were stored at a temperature of 6 degree C. Analyzes were performed to characterize physical (weight, temperature, percentage of losses) and proximate composition (Humidity, fat, protein, ash and weight loss) of A. angustifolia (Bert.) pine seed after three months of storage. The results indicated that there was no significant difference between treatments at protein parameter. About the other parameters there was an increase humidity and decrease with ash and fat with the treatments. (author)

  5. The inhibitory effect of the various seed coating substances against rice seed borne fungi and their shelf-life during storage.

    Science.gov (United States)

    Thobunluepop, Pitipong

    2009-08-15

    Presently, chemical seed treatments are in discussion due to their directly or indirectly impacts on human health or other living organisms. They may also negatively affect the ecosystem and the food chain. In rice seeds, chemicals may cause phytotoxic effects including seed degradation. Eugenol is the main component of clove (Eugenia caryophillis) oil, which was proved to act simultaneously as bactericide, virocide and especially fungicide. The in vitro study was aimed to compare the inhibitory effect of the following seed treatment substances against seed borne fungi and their shelf-life during 12 months of storage; conventional captan (CA), chitosan-lignosulphonate polymer (CL), eugenol incorporated into chitosan-lignosulphonate polymer (E+CL) and control (CO). The obtained results of fungi inhibition were classified in three groups, which showed at first that CA treatment led to a better, i.e., longer, inhibitory effect on Alternaria padwickii, Rhizoctonia solani, Curvularia sp., Aspergillus flavus and Aspergillus niger than E+CL. Secondly, E+CL coating polymer showed the longest inhibitory effect against Bipolaris oryzae and Nigrospora oryzae compared to CA and CL coating polymer. Finally, both CA and E+CL coating polymer had non-significant difference inhibitory effect on Fusarium moniliforme. The variant of CL coating polymer for seed coating was only during the first 6 months of storage able to inhibit all species of the observed seed borne fungi, whereas CA and E+CL coating polymer were capable to inhibit most of the fungi until 9 months of storage.

  6. Characterization of fatty acids, bioactive lipids, and radical scavenging activity of Canterbury bells seed oil

    Directory of Open Access Journals (Sweden)

    Hassanien, M. F.R.

    2014-06-01

    Full Text Available The aim of this study was to characterize the chemical composition and radical scavenging activity of Canterbury bells (Campanula medium seed oil. C. medium seeds contained 9.2% extractable oil. The lipid classes, fatty acids, phytosterol and tocopherol composition of C. medium seed oil were determined. The amount of neutral lipids in the oil was the highest, followed by glycolipids and phospholipids. Linoleic and oleic were the main fatty acids. C. medium oil is characterized by high levels of phytosterols and β-sitosterol was the main compound. β-Tocopherol constituted 42.5% of the total tocopherol content followed by γ-tocopherol. The radical scavenging activity (RSA toward 1,1-diphenyl-2-picrylhydrazyl (DPPH radicals and galvinoxyl radicals of C. medium oil were higher than those of extra virgin olive oil. The diverse potential uses of C. medium oil may make this plant industrially important.El objetivo de este estudio fue caracterizar la composición química y la actividad de captación de radicales de aceites de semillas de campanillas de Canterbury (Campanula medium. Las semillas de C. medium contenían 9,2 % de aceite extraíble. Se determinó la composición de las diferentes clases de lípidos, ácidos grasos, fitoesteroles y tocoferoles. La cantidad de lípidos neutros en el aceite fue mayoritario, seguido de glicolípidos y fosfolípidos. Linoleico y oleico fueron los ácidos grasos principales. El aceite de C. medium se caracteriza por altos niveles de fitoesteroles y β-sitosterol fue el compuesto principal. β-tocoferol constituía 42,5 % del contenido total de tocoferol seguido de γ-tocoferol. La actividad de captación de radicales (RSA a 1,1-difenil-2- picrilhidrazil (DPPH y radicales galvinoxil de C. medium fueron superiores a las de aceite de oliva virgen extra. Los diversos usos potenciales de los aceites de C. medium pueden hacer que esta planta pueda ser importante industrialmente.

  7. CARS microscopy for the monitoring of lipid storage in C. elegans

    Science.gov (United States)

    Enejder, Annika; Brackmann, Christian; Axäng, Claes; Åkeson, Madeleine; Pilon, Marc

    2008-02-01

    After several years of proof-of-principle measurements and focus on technological development, it is timely to make full use of the capabilities of CARS microscopy within the biosciences. We have here identified an urgent biological problem, to which CARS microscopy provides unique insights and consequently may become a widely accepted experimental procedure. In order to improve present understanding of mechanisms underlying dysfunctional metabolism regulation reported for many of our most wide-spread diseases (obesity, diabetes, cardio-vascular diseases etc.), we have monitored genetic and environmental impacts on cellular lipid storage in the model organism C. elegans in vivo in a full-scale biological study. Important advantages of CARS microscopy could be demonstrated compared to present technology, i.e. fluorescence microscopy of labelled lipid stores. The fluorescence signal varies not only with the presence of lipids, but also with the systemic distribution of the fluorophore and the chemical properties of the surrounding medium. By instead probing high-density regions of CH bonds naturally occurring in the sample, the CARS process was shown to provide a consistent representation of the lipid stores. The increased accumulation of lipid stores in mutants with deficiencies in the insulin and transforming growth factor signalling pathways could hereby be visualized and quantified. Furthermore, spectral CARS microscopy measurements in the C-H bond region of 2780-2930 cm -1 provided the interesting observation that this accumulation comes with a shift in the ordering of the lipids from gel- to liquid phase. The present study illustrates that CARS microscopy has a strong potential to become an important instrument for systemic studies of lipid storage mechanisms in living organisms, providing new insights into the phenomena underlying metabolic disorders.

  8. Seed storage protein gene promoters contain conserved DNA motifs in Brassicaceae, Fabaceae and Poaceae

    Science.gov (United States)

    Fauteux, François; Strömvik, Martina V

    2009-01-01

    Background Accurate computational identification of cis-regulatory motifs is difficult, particularly in eukaryotic promoters, which typically contain multiple short and degenerate DNA sequences bound by several interacting factors. Enrichment in combinations of rare motifs in the promoter sequence of functionally or evolutionarily related genes among several species is an indicator of conserved transcriptional regulatory mechanisms. This provides a basis for the computational identification of cis-regulatory motifs. Results We have used a discriminative seeding DNA motif discovery algorithm for an in-depth analysis of 54 seed storage protein (SSP) gene promoters from three plant families, namely Brassicaceae (mustards), Fabaceae (legumes) and Poaceae (grasses) using backgrounds based on complete sets of promoters from a representative species in each family, namely Arabidopsis (Arabidopsis thaliana (L.) Heynh.), soybean (Glycine max (L.) Merr.) and rice (Oryza sativa L.) respectively. We have identified three conserved motifs (two RY-like and one ACGT-like) in Brassicaceae and Fabaceae SSP gene promoters that are similar to experimentally characterized seed-specific cis-regulatory elements. Fabaceae SSP gene promoter sequences are also enriched in a novel, seed-specific E2Fb-like motif. Conserved motifs identified in Poaceae SSP gene promoters include a GCN4-like motif, two prolamin-box-like motifs and an Skn-1-like motif. Evidence of the presence of a variant of the TATA-box is found in the SSP gene promoters from the three plant families. Motifs discovered in SSP gene promoters were used to score whole-genome sets of promoters from Arabidopsis, soybean and rice. The highest-scoring promoters are associated with genes coding for different subunits or precursors of seed storage proteins. Conclusion Seed storage protein gene promoter motifs are conserved in diverse species, and different plant families are characterized by a distinct combination of conserved motifs

  9. Seed storage protein gene promoters contain conserved DNA motifs in Brassicaceae, Fabaceae and Poaceae

    Directory of Open Access Journals (Sweden)

    Fauteux François

    2009-10-01

    Full Text Available Abstract Background Accurate computational identification of cis-regulatory motifs is difficult, particularly in eukaryotic promoters, which typically contain multiple short and degenerate DNA sequences bound by several interacting factors. Enrichment in combinations of rare motifs in the promoter sequence of functionally or evolutionarily related genes among several species is an indicator of conserved transcriptional regulatory mechanisms. This provides a basis for the computational identification of cis-regulatory motifs. Results We have used a discriminative seeding DNA motif discovery algorithm for an in-depth analysis of 54 seed storage protein (SSP gene promoters from three plant families, namely Brassicaceae (mustards, Fabaceae (legumes and Poaceae (grasses using backgrounds based on complete sets of promoters from a representative species in each family, namely Arabidopsis (Arabidopsis thaliana (L. Heynh., soybean (Glycine max (L. Merr. and rice (Oryza sativa L. respectively. We have identified three conserved motifs (two RY-like and one ACGT-like in Brassicaceae and Fabaceae SSP gene promoters that are similar to experimentally characterized seed-specific cis-regulatory elements. Fabaceae SSP gene promoter sequences are also enriched in a novel, seed-specific E2Fb-like motif. Conserved motifs identified in Poaceae SSP gene promoters include a GCN4-like motif, two prolamin-box-like motifs and an Skn-1-like motif. Evidence of the presence of a variant of the TATA-box is found in the SSP gene promoters from the three plant families. Motifs discovered in SSP gene promoters were used to score whole-genome sets of promoters from Arabidopsis, soybean and rice. The highest-scoring promoters are associated with genes coding for different subunits or precursors of seed storage proteins. Conclusion Seed storage protein gene promoter motifs are conserved in diverse species, and different plant families are characterized by a distinct combination

  10. Role of relative humidity in processing and storage of seeds and assessment of variability in storage behaviour in Brassica spp. and Eruca sativa.

    Science.gov (United States)

    Suma, A; Sreenivasan, Kalyani; Singh, A K; Radhamani, J

    2013-01-01

    The role of relative humidity (RH) while processing and storing seeds of Brassica spp. and Eruca sativa was investigated by creating different levels of relative humidity, namely, 75%, 50%, 32%, and 11% using different saturated salt solutions and 1% RH using concentrated sulphuric acid. The variability in seed storage behaviour of different species of Brassica was also evaluated. The samples were stored at 40 ± 2°C in sealed containers and various physiological parameters were assessed at different intervals up to three months. The seed viability and seedling vigour parameters were considerably reduced in all accessions at high relative humidity irrespective of the species. Storage at intermediate relative humidities caused minimal decline in viability. All the accessions performed better at relative humidity level of 32% maintaining seed moisture content of 3%. On analyzing the variability in storage behaviour, B. rapa and B. juncea were better performers than B. napus and Eruca sativa.

  11. Influence of dietary lipid and protein sources on the sensory quality of organic rainbow trout (Oncorhynchus mykiss) after ice storage

    DEFF Research Database (Denmark)

    Green-Petersen, Ditte; Hyldig, Grethe; Jacobsen, Charlotte

    2014-01-01

    The influence of dietary protein and lipid sources on the quality of organic rainbow trout (Oncorhynchus mykiss) was studied. The protein and oil sources were fishmeal, fish oil, and organic vegetable protein and oils. Sensory profiling was performed during 3 to 14 days of ice storage along...... with lipid analyses of the fillet. Overall, the results showed that the sensory characteristics of the trout were affected in different ways during ice storage. The source of lipid seemed to affect the sensory quality at the beginning of the storage period, while the protein source seemed to have a more...

  12. Neutral lipid-storage disease with myopathy and extended phenotype with novel PNPLA2 mutation.

    Science.gov (United States)

    Massa, Roberto; Pozzessere, Simone; Rastelli, Emanuele; Serra, Laura; Terracciano, Chiara; Gibellini, Manuela; Bozzali, Marco; Arca, Marcello

    2016-04-01

    Neutral lipid-storage disease with myopathy is caused by mutations in PNPLA2, which produce skeletal and cardiac myopathy. We report a man with multiorgan neutral lipid storage and unusual multisystem clinical involvement, including cognitive impairment. Quantitative brain MRI with voxel-based morphometry and extended neuropsychological assessment were performed. In parallel, the coding sequences and intron/exon boundaries of the PNPLA2 gene were screened by direct sequencing. Neuropsychological assessment revealed global cognitive impairment, and brain MRI showed reduced gray matter volume in the temporal lobes. Molecular characterization revealed a novel homozygous mutation in exon 5 of PNPLA2 (c.714C>A), resulting in a premature stop codon (p.Cys238*). Some PNPLA2 mutations, such as the one described here, may present with an extended phenotype, including brain involvement. In these cases, complete neuropsychological testing, combined with quantitative brain MRI, may help to characterize and quantify cognitive impairment. © 2016 Wiley Periodicals, Inc.

  13. Optimization of Cultivation and Storage Conditions on Red Cabbage Seed Sprouts

    International Nuclear Information System (INIS)

    Baek, K.H.; Jo, D.J.; Park, J.H.; Kwon, J.H.; Kim, G.R.; Lee, G.D.; Kim, J.S.; Kim, Y.R.; Han, B.S.; Yoon, S.R.

    2013-01-01

    This study was carried out to find the optimal conditions for red cabbage seed sprouts in terms of their physicochemical and sensory qualities by electron-beam irradiation, cultivation and storage using the response surface methodology (RSM). Moisture content (R2 = 0.9638) was affected by irradiation dose and cultivation time. Total phenolics content (R2 = 0.9117) was mainly affected by irradiation dose, but carotenoid content (R2 = 0.8338) was affected in the order of irradiation dose, cultivation time and storage time. Sensory properties were also affected by irradiation dose, and thus scores decreased as irradiation dose increased. The optimum conditions estimated by superimposing total phenolics content and overall acceptance were 2.2-3.8 kGy of the irradiation dose, 3.0-4.0 days of cultivation and 2.0-3.0 days of storage

  14. Impaired Autophagy in the Lipid-Storage Disorder Niemann-Pick Type C1 Disease

    OpenAIRE

    Sarkar, Sovan; Carroll, Bernadette; Buganim, Yosef; Maetzel, Dorothea; Ng, Alex H.M.; Cassady, John P.; Cohen, Malkiel A.; Chakraborty, Souvik; Wang, Haoyi; Spooner, Eric; Ploegh, Hidde; Gsponer, Joerg; Korolchuk, Viktor I.; Jaenisch, Rudolf

    2013-01-01

    Autophagy dysfunction has been implicated in misfolded protein accumulation and cellular toxicity in several diseases. Whether alterations in autophagy also contribute to the pathology of lipid-storage disorders is not clear. Here, we show defective autophagy in Niemann-Pick type C1 (NPC1) disease associated with cholesterol accumulation, where the maturation of autophagosomes is impaired because of defective amphisome formation caused by failure in SNARE machinery, whereas the lysosomal prot...

  15. Fatty Acid Composition and Lipid Profile of Diospyros mespiliformis, Albizia lebbeck, and Caesalpinia pulcherrima Seed Oils from Nigeria.

    Science.gov (United States)

    Adewuyi, Adewale; Oderinde, Rotimi Ayodele

    2014-01-01

    The screening of lesser-known underutilized seeds as source of food has been a way of finding solution to food insecurity in developing nations. In this regard, oil as a class of food was extracted from the seeds of Diospyros mespiliformis  (4.72 ± 0.2%), Albizia lebbeck  (6.40 ± 0.60%), and Caesalpinia pulcherrima  (7.2 ± 0.30%). The oils were finally analyzed for their fatty acid composition, lipid classes, fatty acid distribution in the lipid fractions, and molecular speciation of the triacylglycerols, glycolipids, and phospholipids. The fatty acid composition of the oils varied with C18:2 fatty acid being the most dominant in the oils. Neutral lipids were the most abundant lipid class found in the oils while molecular species of the triacylglycerol with equivalent carbon chain number C40 was majorly present in the oils of Diospyros mespiliformis and Caesalpinia pulcherrima. The present study presents lesser-known underutilized seeds as possible sources of food.

  16. Fatty Acid Composition and Lipid Profile of Diospyros mespiliformis, Albizia lebbeck, and Caesalpinia pulcherrima Seed Oils from Nigeria

    Directory of Open Access Journals (Sweden)

    Adewale Adewuyi

    2014-01-01

    Full Text Available The screening of lesser-known underutilized seeds as source of food has been a way of finding solution to food insecurity in developing nations. In this regard, oil as a class of food was extracted from the seeds of Diospyros mespiliformis  (4.72±0.2%, Albizia lebbeck  (6.40±0.60%, and Caesalpinia pulcherrima  (7.2±0.30%. The oils were finally analyzed for their fatty acid composition, lipid classes, fatty acid distribution in the lipid fractions, and molecular speciation of the triacylglycerols, glycolipids, and phospholipids. The fatty acid composition of the oils varied with C18:2 fatty acid being the most dominant in the oils. Neutral lipids were the most abundant lipid class found in the oils while molecular species of the triacylglycerol with equivalent carbon chain number C40 was majorly present in the oils of Diospyros mespiliformis and Caesalpinia pulcherrima. The present study presents lesser-known underutilized seeds as possible sources of food.

  17. A lipid storage-like disorder contributes to cognitive decline in HIV-infected subjects.

    Science.gov (United States)

    Bandaru, Veera Venkata Ratnam; Mielke, Michelle M; Sacktor, Ned; McArthur, Justin C; Grant, Igor; Letendre, Scott; Chang, Linda; Wojna, Valerie; Pardo, Carlos; Calabresi, Peter; Munsaka, Sody; Haughey, Norman J

    2013-10-22

    In this multicenter cohort study, we sought to identify prognostic and associative metabolic indicators for HIV-associated neurocognitive disorders (HAND). A quantitative lipidomic analysis was conducted on 524 longitudinal CSF samples collected from 7 different performance sites across the mainland United States, Hawaii, and Puerto Rico. Subjects included HIV-infected individuals with longitudinal clinical and cognitive testing data and cognitively normal HIV-negative healthy controls. At baseline, HIV+ subjects could be differentiated from HIV- controls by reductions in a single ceramide species and increases in multiple forms of cholesterol. Perturbations in cholesterol metabolism and ceramide were influenced by combined antiretroviral therapy (cART) use. There were no cross-sectional baseline differences in any lipid metabolite when HIV+ subjects were grouped according to cognitive status. However, a single sphingolipid metabolite and reduced levels of esterified cholesterols were prognostic indicators of incident cognitive decline. Longitudinal patterns of these disturbances in sphingolipid and sterol metabolism suggest that a progressive disorder of lipid metabolism that is similar to disorders of lipid storage may contribute to the pathogenesis of HAND. These findings suggest that HIV infection and cART are independently associated with a CNS metabolic disturbance, identify surrogate markers that are prognostic for cognitive decline, and implicate a lipid storage-like disorder in the progression of HAND.

  18. Sugar versus fat: elimination of glycogen storage improves lipid accumulation in Yarrowia lipolytica.

    Science.gov (United States)

    Bhutada, Govindprasad; Kavšcek, Martin; Ledesma-Amaro, Rodrigo; Thomas, Stéphane; Rechberger, Gerald N; Nicaud, Jean-Marc; Natter, Klaus

    2017-05-01

    Triacylglycerol (TAG) and glycogen are the two major metabolites for carbon storage in most eukaryotic organisms. We investigated the glycogen metabolism of the oleaginous Yarrowia lipolytica and found that this yeast accumulates up to 16% glycogen in its biomass. Assuming that elimination of glycogen synthesis would result in an improvement of lipid accumulation, we characterized and deleted the single gene coding for glycogen synthase, YlGSY1. The mutant was grown under lipogenic conditions with glucose and glycerol as substrates and we obtained up to 60% improvement in TAG accumulation compared to the wild-type strain. Additionally, YlGSY1 was deleted in a background that was already engineered for high lipid accumulation. In this obese background, TAG accumulation was also further increased. The highest lipid content of 52% was found after 3 days of cultivation in nitrogen-limited glycerol medium. Furthermore, we constructed mutants of Y. lipolytica and Saccharomyces cerevisiae that are deleted for both glycogen and TAG synthesis, demonstrating that the ability to store carbon is not essential. Overall, this work showed that glycogen synthesis is a competing pathway for TAG accumulation in oleaginous yeasts and that deletion of the glycogen synthase has beneficial effects on neutral lipid storage. © FEMS 2017.

  19. Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo

    Directory of Open Access Journals (Sweden)

    Ladislav Nedbal

    2010-09-01

    Full Text Available Algae are becoming a strategic source of fuels, food, feedstocks, and biologically active compounds. This potential has stimulated the development of innovative analytical methods focused on these microorganisms. Algal lipids are among the most promising potential products for fuels as well as for nutrition. The crucial parameter characterizing the algal lipids is the degree of unsaturation of the constituent fatty acids quantified by the iodine value. Here we demonstrate the capacity of the spatially resolved Raman microspectroscopy to determine the effective iodine value in lipid storage bodies of individual living algal cells. The Raman spectra were collected from three selected algal species immobilized in an agarose gel. Prior to immobilization, the algae were cultivated in the stationary phase inducing an overproduction of lipids. We employed the characteristic peaks in the Raman scattering spectra at 1,656 cm−1 (cis C=C stretching mode and 1,445 cm−1 (CH2 scissoring mode as the markers defining the ratio of unsaturated-to-saturated carbon-carbon bonds of the fatty acids in the algal lipids. These spectral features were first quantified for pure fatty acids of known iodine value. The resultant calibration curve was then used to calculate the effective iodine value of storage lipids in the living algal cells from their Raman spectra. We demonstrated that the iodine value differs significantly for the three studied algal species. Our spectroscopic estimations of the iodine value were validated using GC-MS measurements and an excellent agreement was found for the Trachydiscus minutus species. A good agreement was also found with the earlier published data on Botryococcus braunii. Thus, we propose that Raman microspectroscopy can become technique of choice in the rapidly expanding field of algal biotechnology.

  20. Proteomic Analysis of Lettuce Seed Germination and Thermoinhibition by Sampling of Individual Seeds at Germination and Removal of Storage Proteins by Polyethylene Glycol Fractionation1

    Science.gov (United States)

    Song, Bin-Yan; Deng, Zhi-Jun; Wang, Yue; Liu, Shu-Jun; Møller, Ian Max; Song, Song-Quan

    2015-01-01

    Germination and thermoinhibition in lettuce (Lactuca sativa ‘Jianyexianfeng No. 1’) seeds were investigated by a proteomic comparison among dry seeds, germinated seeds at 15°C, at 15°C after imbibition at 25°C for 48 h, or at 25°C in KNO3 (all sampled individually at germination), and ungerminated seeds at 25°C, a thermoinhibitory temperature. Before two-dimensional gel electrophoresis analysis, storage proteins (greater than 50% of total extractable protein) were removed by polyethylene glycol precipitation, which significantly improved the detection of less abundant proteins on two-dimensional gels. A total of 108 protein spots were identified to change more than 2-fold (P lettuce seed germination and thermoinhibition. Accumulation of three proteins and expression of five genes participating in the mevalonate (MVA) pathway of isoprenoid biosynthesis correlated positively with seed germinability. Inhibition of this pathway by lovastatin delayed seed germination and increased the sensitivity of germination to abscisic acid. MVA pathway-derived products, cytokinins, partially reversed the lovastatin inhibition of germination and released seed thermoinhibition at 25°C. We conclude that the MVA pathway for isoprenoid biosynthesis is involved in lettuce seed germination and thermoinhibition. PMID:25736209

  1. Effects of origin, seasons and storage under different temperatures on germination of Senecio vulgaris (Asteraceae) seeds.

    Science.gov (United States)

    Ndihokubwayo, Noel; Nguyen, Viet-Thang; Cheng, Dandan

    2016-01-01

    Invasive plants colonize new environments, become pests and cause biodiversity loss, economic loss and health damage. Senecio vulgaris L. (Common groundsel, Asteraceae), a widely distributing cosmopolitan weed in the temperate area, is reported with large populations in the north-eastern and south-western part, but not in southern, central, or north-western parts of China. We studied the germination behavior of S. vulgaris to explain the distribution and the biological invasion of this species in China. We used seeds originating from six native and six invasive populations to conduct germination experiments in a climate chamber and under outdoor condition. When incubated in a climate chamber (15 °C), seeds from the majority of the populations showed >90% germination percentage (GP) and the GP was equal for seeds with a native and invasive origin. The mean germination time (MGT) was significantly different among the populations. Under outdoor conditions, significant effects of origin, storage conditions (stored at 4 °C or ambient room temperature, ca. 27 °C) and seasons (in summer or autumn) were observed on the GP while the MGT was only affected by the season. In autumn, the GP (38.6%) was higher and the MGT was slightly longer than that in summer. In autumn, seeds stored at 4 °C showed higher GP than those stored at ambient room temperature (ca.27 °C), and seeds from invasive populations revealed higher GP than those from native populations. The results implied that the high temperature in summer has a negative impact on the germination and might cause viability loss or secondary dormancy to S. vulgaris seeds. Our study offers a clue to exploring what factor limits the distribution of S. vulgaris in China by explaining why, in the cities in South-East China and central China such as Wuhan, S. vulgaris cannot establish natural and viable populations.

  2. Effects of origin, seasons and storage under different temperatures on germination of Senecio vulgaris (Asteraceae seeds

    Directory of Open Access Journals (Sweden)

    Noel Ndihokubwayo

    2016-08-01

    Full Text Available Invasive plants colonize new environments, become pests and cause biodiversity loss, economic loss and health damage. Senecio vulgaris L. (Common groundsel, Asteraceae, a widely distributing cosmopolitan weed in the temperate area, is reported with large populations in the north–eastern and south–western part, but not in southern, central, or north-western parts of China. We studied the germination behavior of S. vulgaris to explain the distribution and the biological invasion of this species in China. We used seeds originating from six native and six invasive populations to conduct germination experiments in a climate chamber and under outdoor condition. When incubated in a climate chamber (15 °C, seeds from the majority of the populations showed >90% germination percentage (GP and the GP was equal for seeds with a native and invasive origin. The mean germination time (MGT was significantly different among the populations. Under outdoor conditions, significant effects of origin, storage conditions (stored at 4 °C or ambient room temperature, ca. 27 °C and seasons (in summer or autumn were observed on the GP while the MGT was only affected by the season. In autumn, the GP (38.6% was higher and the MGT was slightly longer than that in summer. In autumn, seeds stored at 4 °C showed higher GP than those stored at ambient room temperature (ca.27 °C, and seeds from invasive populations revealed higher GP than those from native populations. The results implied that the high temperature in summer has a negative impact on the germination and might cause viability loss or secondary dormancy to S. vulgaris seeds. Our study offers a clue to exploring what factor limits the distribution of S. vulgaris in China by explaining why, in the cities in South-East China and central China such as Wuhan, S. vulgaris cannot establish natural and viable populations.

  3. Development of C-lignin with G/S-lignin and lipids in orchid seed coats – an unexpected diversity exposed by ATR-FT-IR spectroscopy

    DEFF Research Database (Denmark)

    Barsberg, Søren Talbro; Lee, Y.-I.; Rasmussen, Hanne Nina

    2018-01-01

    Cite this article: Barsberg ST, Lee Y-I, Rasmussen HN. Development of C-lignin with G/S-lignin and lipids in orchid seed coats – an unexpected diversity exposed by ATR-FT-IR spectroscopy. Seed Science Research https:// doi.org/10.1017/S0960258517000344......Cite this article: Barsberg ST, Lee Y-I, Rasmussen HN. Development of C-lignin with G/S-lignin and lipids in orchid seed coats – an unexpected diversity exposed by ATR-FT-IR spectroscopy. Seed Science Research https:// doi.org/10.1017/S0960258517000344...

  4. Rapid screening for lipid storage disorders using biochemical markers Expert center data and review of the literature

    NARCIS (Netherlands)

    Voorink-Moret, M.; Goorden, S. M. I.; van Kuilenburg, A. B. P.; Wijburg, F. A.; Ghauharali-van der Vlugt, J. M. M.; Beers-Stet, F. S.; Zoetekouw, A.; Kulik, W.; Hollak, C. E. M.; Vaz, F. M.

    2018-01-01

    Background: In patients suspected of a lipid storage disorder (sphingolipidoses, lipidoses), confirmation of the diagnosis relies predominantly on the measurement of specific enzymatic activities and genetic studies. New UPLC-MS/MS methods have been developed to measure lysosphingolipids and

  5. Essential oil and their microconstituents of cumin and coriander seeds during storage under the effect of gamma irradiation

    International Nuclear Information System (INIS)

    Sharabash, M.T.M.; Abdullah, M.I.

    1999-01-01

    Both cumin and coriander seeds were treated with gamma irradiation (10 KGy). The volatile oil and micro constituents were identified over seven months of storage. No marked changes were noticed regarding the volatile oil content and oil components between irradiated and non-irradiated of the two spices during the storage period at egyptian ambient temperature

  6. GIBBERELLINS, FUNGICIDES AND STORAGE EFFECTS ON THE GERMINATION OF Genipa americana L. (RUBIACEAE SEEDS

    Directory of Open Access Journals (Sweden)

    Fábio de Almeida Vieira

    2006-06-01

    Full Text Available The aim of this paper was to verify the effect of different doses of gibberellic acid (GA3 (0, 250, 500, 750 and 1000 µg.L-1, of fungicides of the groups chemical benzimidazol (0, 25, 50 and 100 g.L-1 and ditiocarbamato (0, 1,25, 2,50 and 5,00 g.L-1 on seed germination. Viability of those seeds was evaluated through germination tests at 0, 15, 30 and 60 days. The experiment was carried out in greenhouse. The experimental design was fully randomized one, with five replicates per treatment. The traits evaluated were emergence and index of emergence speed. The treatment with GA3 didn't provide significant so much differences among the germination rates as well as for the emergence speed. It was verified that the use of the fungicides in smaller concentrations (25 g.L-1 of benzimidazol and 1,25 g.L-1 of ditiocarbamato promoted a better germination speed. The seeds of G. americana possess viability period relatively short, with germination absence 60 days period of storage, and it could be associated to the humidity tenors presented by the seeds in this period.

  7. Opposed-phase MR imaging of lipid storage myopathy in a case of Chanarin-Dorfman disease

    International Nuclear Information System (INIS)

    Gaeta, Michele; Celona, Antonio; Racchiusa, Sergio; Mazziotti, Silvio; Minutoli, Fabio; Toscano, Antonio; Musumeci, Olimpia

    2008-01-01

    Chanarin-Dorfman disease (CDD) is a rare genetic disorder characterized by ichthyosis, myopathy, central nervous system disturbances, and intracellular lipid storage in muscle fibers, hepatocytes, and granulocytes. We describe skeletal muscle magnetic resonance imaging findings in a case of CDD, outlining the potential role of GE T1-weighted opposed-phase sequence (chemical shift imaging) in the evaluation of lipid storage myopathies. (orig.)

  8. Opposed-phase MR imaging of lipid storage myopathy in a case of Chanarin-Dorfman disease

    Energy Technology Data Exchange (ETDEWEB)

    Gaeta, Michele; Celona, Antonio; Racchiusa, Sergio; Mazziotti, Silvio [University of Messina, Department of Radiological Sciences, Messina (Italy); Minutoli, Fabio [University of Messina, Department of Radiological Sciences, Messina (Italy); A.O.U. ' ' Policlinico G. Martino' ' , Dipartimento di Scienze Radiologiche, Messina (Italy); Toscano, Antonio; Musumeci, Olimpia [University of Messina, Department of Neurosciences, Psychiatry and Anaesthesiology, Messina (Italy)

    2008-11-15

    Chanarin-Dorfman disease (CDD) is a rare genetic disorder characterized by ichthyosis, myopathy, central nervous system disturbances, and intracellular lipid storage in muscle fibers, hepatocytes, and granulocytes. We describe skeletal muscle magnetic resonance imaging findings in a case of CDD, outlining the potential role of GE T1-weighted opposed-phase sequence (chemical shift imaging) in the evaluation of lipid storage myopathies. (orig.)

  9. Effects of storage conditions of Moringa oleifera seeds on its performance in coagulation.

    Science.gov (United States)

    Katayon, S; Noor, M J Megat Mohd; Asma, M; Ghani, L A Abdul; Thamer, A M; Azni, I; Ahmad, J; Khor, B C; Suleyman, A M

    2006-09-01

    Moringa oleifera is a plant whose seeds have coagulation properties for treating water and wastewater. In this study the coagulation efficiency of Moringa oleifera kept in different storage conditions were studied. The Moringa oleifera seeds were stored at different conditions and durations; open container and closed container at room temperature (28 degrees C) and refrigerator (3 degrees C) for durations of 1, 3 and 5 months. Comparison between turbidity removal efficiency of Moringa oleifera kept in refrigerator and room temperature revealed that there was no significant difference between them. The Moringa oleifera kept in refrigerator and room temperature for one month showed higher turbidity removal efficiency, compared to those kept for 3 and 5 months, at both containers. The coagulation efficiency of Moringa oleifera was found to be dependent on initial turbidity of water samples. Highest turbidity removals were obtained for water with very high initial turbidity. In summary coagulation efficiency of Moringa oleifera was found independent of storage temperature and container, however coagulation efficiency of Moringa oleifera decreased as storage duration increased. In addition, Moringa oleifera can be used as a potential coagulant especially for very high turbidity water.

  10. Effects of Storage and Exogenous Ga3 on Lychee Seed Germination

    Directory of Open Access Journals (Sweden)

    Elizabeth Orika Ono

    2000-01-01

    Full Text Available The effects of storage time and exogenous gibberellic acid on lychee seeds germination were studied. The seeds were removed from ripe fruits, washed, dried, stored at 8°C for 0, 15 and 30 days, and soaked during 24 hours in water and GA3 at 50, 100 and 200 mg.L-1 solutions. As the storage period increased, the germination capacity was lost, and the seeds had a short germinability after 30-day storage period. Gibberellic acid had not significant effect on enhancing both the germination percentage and rate.O trabalho teve como objetivo estudar os efeitos do tempo de armazenamento e de tratamentos com ácido giberélico, no processo germinativo de sementes de lichieira (Litchi chinensis Sonn.. As sementes foram retiradas de frutos maduros, lavadas, secas à sombra e colocadas para germinar imediatamente ou então, armazenadas em geladeira (8°C por 15 e 30 dias. Os tratamentos corresponderam à imersão das sementes por 24 horas nas seguintes soluções com aeração: água, GA3 a 50, 100 e 200 mg.L-1. Através dos resultados obtidos, observou-se que as sementes perderam o poder germinativo, à medida que aumentou-se o tempo de armazenamento, sendo a porcentagem de germinação muito baixa (7% aos 30 dias de armazenamento. O tempo médio de germinação foi menor após 15 dias de armazenamento.

  11. Arabidopsis lipid droplet-associated protein (LDAP)–interacting protein (LDIP) influences lipid droplet size and neutral lipid homeostasis in both leaves and seeds

    Science.gov (United States)

    Cytoplasmic lipid droplets (LDs) are found in all types of plant cells where they are derived from the endoplasmic reticulum and function as a repository for neutral lipids, as well as serving in lipid remodelling and signalling. However, the mechanisms underlying the formation and functioning of pl...

  12. Evaluation of Clausena pentaphylla (Roxb.) DC oil as a fungitoxicant against storage mycoflora of pigeon pea seeds.

    Science.gov (United States)

    Pandey, Abhay K; Palni, Uma T; Tripathi, Nijendra N

    2013-05-01

    The present study aimed to evaluate the antifungal activity of 30 essential oils against four dominant fungi Aspergillus flavus Link., A. niger van Tieghem, A. ochraceus Wilhelm and A. terreus Thom of stored pigeon pea seeds at a concentration of 0.36 µL mL(-1). Various fungitoxic properties, such as minimum inhibitory concentration, minimum fungicidal concentration and fungitoxic spectrum, of the most potent oil were determined. The efficacy of the most potent oil in preservation of pigeon pea seeds for 6 months was also carried out by storing 1 kg of seeds in the oil vapour. Clausena pentaphylla and Citrus limon oils were more effective against all the fungi tested, which exhibited 100% per cent mycelial inhibition. The minimum inhibitory concentration of C. pentaphylla oil was determined as 0.07 µL mL(-1) against all the test fungi and was found to be more toxic than Citrus limon oil. C. pentaphylla oil exhibited a broad range of fungitoxicity against 16 other storage fungi of pigeon pea seeds. C. pentaphylla oil significantly protected 1 kg seeds of pigeon pea from fungal deterioration and was superior to synthetic fumigants. The oil did not show any phytotoxicity and the protein content of the seeds was significantly retained for up to 6 months of storage. Thus, C. pentaphylla oil may be used as an effective fumigant in the ecofriendly management of storage fungi of pigeon pea seeds. © 2012 Society of Chemical Industry.

  13. Application of neem (Azadirachta indica) as biological pesticides in cocoa seed (Theobroma cacao) storage using various local adsorbent media

    Science.gov (United States)

    Mardiyani, S. A.; Sunawan; Pawestri, A. E.

    2018-03-01

    Cocoa seeds are recalcitrant (the water content is more than 40%) that require special handling. The use of adsorbent media to reduce the decrease in the quality of cocoa seeds and extend their shelf life in this storage has not been widely done. Local adsorbent media such as sawdust, sand and ash have the potential to maintain the viability of cocoa seeds. The objective of this research was to determine the interaction of the application of neem (Azadirachta indica) as biological pesticides and the use of various natural adsorbent media in the storage of cocoa seeds (Theobroma cacao). It was an experimental study with a factorial design composed of three factors. The first factor was the medium adsorbent type for the storage of cocoa seed, which consists of three levels (river sand, ash, and sawdust). The second factor was the concentration of neem leaves for pre-storage treatment with three levels (10, 20, and 30%). The third factor was the storage time (10 and 20 days). The results of the study indicated that the combination of the three factors showed a significant interaction in the height of the plant and the diameter of the stem of the seedling at 28 days after sowing. The fresh weight of the seedlings of the seeds that were stored in ash media gave a better result than the seedlings of seeds that had been stored in the river sand and the sawdust as adsorbent media. The application of 20% extract of neem leaves gave the best influence for the seeds that were stored for 20 days.

  14. Lipid oxidative changes in traditional dry fermented sausage Petrovská klobása during storage

    OpenAIRE

    Šojić, Branislav V.; Petrović, Ljiljana S.; Mandić, Anamarija I.; Sedej, Ivana J.; Džinić, Natalija R.; Tomović, Vladimir M.; Jokanović, Marija R.; Tasić, Tatjana A.; Škaljac, Snežana B.; Ikonić, Predrag M.

    2014-01-01

    The influence of drying and ripeninig conditions (traditional and industrial) in the production of dry fermented sausage Petrovská klobása, on fatty-acid composition and oxidative changes in lipids, during 7 months of storage, was investigated. During the storage period, the sum of unsaturated fatty acids and the content of free fatty acids were significantly higher (p

  15. Effect of annatto seed and coriander leaves as natural antioxidants in fish meatballs during frozen storage.

    Science.gov (United States)

    Sancho, Renata Aparecida Soriano; de Lima, Fabíola Aliaga; Costa, Gabriel Guerra; Mariutti, Lilian Regina Barros; Bragagnolo, Neura

    2011-08-01

    The effects of annatto (0.1 g/100g) and coriander (0.5 g/100g) were assessed against lipid oxidation in white hake meatballs cooked in boiling water (95 ± 1 °C) for 30 min and stored at -18 °C for 120 d. The fatty acids (FA) and the nutritional quality, cholesterol, cholesterol oxides, thiobarbituric acid reactive substances (TBARS) values, and conjugated dienes were analyzed to follow the course of oxidation. Annatto and coriander were efficient in the control of lipid oxidation, also preserving the essential FA. At 120 d of storage, the eicosapentaenoic acid (EPA) concentration decreased respectively by 43%, 32%, 12%, and 9% in the control, coriander, annatto, and annatto + coriander patties. For docosahexaenoic acid (DHA), these concentrations decreased, respectively, 44%, 30%, 11%, and 7%, revealing a probable synergistic effect among the antioxidant compounds present in both spices. On the other hand, annatto and coriander were not able to act protecting the meatballs against lipid oxidation when they were cooked, also not exerted any effect in the cholesterol oxidation. Spices, especially coriander and annatto, can be an alternative to substitute synthetic additives with antioxidants to prevent loss of important unsaturated fatty acids (UFA) in fish meatballs during frozen storage for 120 d. The maximum effect was observed when 0.5% coriander and 0.1% annatto were used in combination. Cooking did not induce the formation of cholesterol oxides, compounds that can cause more health damages than cholesterol itself; however, during storage the cholesterol oxides levels presented a little increase regardless of spice addition. © 2011 Institute of Food Technologists®

  16. Enhancing Lipid Stability in Irradiated Beef Mince by Oleoresins and/ or Ascorbic Acid during Chilling Storage

    International Nuclear Information System (INIS)

    Zahran, D.A.

    2008-01-01

    Lipid Oxidation, fatty acids profile and sensory properties of irradiated beef mince (2.5 kGy) treated with oleoresins (rosemary or ginger), ascorbic acid, or combination of ascorbic acid and oleoresins were investigated during 30 days of chilled storage. Thiobarbituric acid reactive substances (TBARS) as an indication of lipid oxidation, of irradiated control samples were significantly higher than those of non irradiated control and samples treated with rosemary and ginger oleoresins. By GC-MS analysis, it was found that the relative percentage of total saturated fatty acids (TSFA) increased in all treatments. However, the highest increase was recorded in irradiated control samples compared to non irradiated control samples. Beef mince samples treated with oleoresins (rosemary or ginger) had the best scores for discoloration and off odour. Thus, the addition of oleoresins (rosemary or ginger) to beef mince before irradiation could be an easily applied method to minimize oxidative degradation of irradiated meat

  17. Studies on the lipid constituents of grape seeds recovered from pomace resulting from white grape processing

    Directory of Open Access Journals (Sweden)

    El-Shami, S. M.

    1992-06-01

    Full Text Available Studies on the lipid constituents of grape seeds, produced as by-product from white grape processing, were carried out. Gas liquid chromatography was used to determine the composition of fatty acid methyl esters and sterol silyl derivatives of the oil. The oil was found to contain appreciable amount of unsaturated fatty acids, namely, oleic and linoleic acids, however, linolenic acid was not detected. Saturated fatty acids, namely, palmitic and stearic were found to be present in reasonable amounts. Myristic, palmitoleic, eicosanoic and eicosadienoic acids were detected as minor components. Isofucosterol was found among the other sterol constituents namely, campesterol, stigmasterol and beta-sitosterol. HPLC analysis of the oil for determination of tocopherols, showed the presence of alpha-and gamma-tocotrienols, and alpha-and gamma-tocopherols to the extent of 53.2, 28.6, 16,4 and 1,8% respectively. The total tocopherols content of the oil was 470 ppm. The defatted meal of grape seeds was found to contain 24,6% protein which contained large proportions of all the essential amino acids as determined by amino acid analyzer.

    Se han realizado estudios sobre los constituyentes lipidiaos de semillas de uva producidas como subproducto del procesado de uva blanca. La cromatografía gas-líquido se usó para determinar la composición de esteres metílicos de ácidos grasos y silil derivados esteroles del aceite. Se observó que el aceite contenía cantidades apreciables de ácidos grasos insaturados, principalmente ácidos oleico y linoleico, no siendo sin embargo detectado el ácido linolénico. Se encontraron en cantidades razonables ácidos grasos saturados, principalmente, palmítico y esteárico. Como componentes minoritarios se detectaron los ácidos mirístico, palmitoleico, eicosanoico y eicosadienoico. El isofucosterol apareció junto a otros esteroles, principalmente, campesterol, estigmasterol y beta-sitosterol. El análisis por HPLC

  18. Effects of storage and soaking on wheat seeds exposed to gamma rays

    International Nuclear Information System (INIS)

    Ajayi, N.O.

    1975-07-01

    Wheat seeds, variety Starke II, of water content 11,5 per cent of dry weight, irradiated with 60 Co gamma rays over a wide range of absorbed doses were sowed in wet sand. When analysed after eight days, seedling growth was found to have decreased with increasing dose, very rapidly within the first 40 krad and more slowly thereafter until that dose, 500 krad, which made the shoot and the root fail to develop. Storage over three weeks was found to produce greater reduction in the seelding growth at doses below 40 krad, and lowering of the dose at which the seedling failed to develop from about 500 krad to 300 krad. Soaking the irradiated seeds immediately after irradiation for one hour before planting was found to improve the seedling growth in the low dose region compared to when seeds were immediately sowed. It is suggested that the water absorbed during soaking permits the free radicals to recombine and DNA-damage to be repaired. It is thought that radiation seriously affects cellular multiplication and the production of growth promoting homones of the embryo. On the other hand elongation of surviving, presumably non-mitotic cells, is apparently taking place even at high doses, in fact up to 500 krad. (author)

  19. Control of quality and silo storage of sunflower seeds using near infrared technology

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez-Martin, I.; Vilaescusa-Garcia, V.; Lopez-Gonzalez, F.; Oiz-Jimenez, C.; Lobos-Ortega, I. A.; Gordillo, B.; Hernandez-Hierro, J. M.

    2013-05-01

    This work assesses the application of near infrared spectroscopy technology for the quality control of sunflower seeds direct from farmers and from a storage silo. The results show that the analytical method employing near infrared spectroscopy can be used as a rapid and non-destructive tool for the determination of moisture, fat and high/low oleic acid contents in samples of sunflower seeds. The ranges obtained were comparable to those reported for classic chemical methods, and were between 4.6-21.4% for moisture; 38.4-49.6% for fat, and 60.0-93.1% for oleic acid expressed as percentage of total fatty acids. A stepwise discriminant analysis was performed to determine the most useful wavelengths for classifying sunflower seeds in terms of their (high/low) oleic acid composition. The discriminant model allows the acid contents, with a prediction rate of 90.5% for internal validation and of 89.4% for cross-validation. (Author) 23 refs.

  20. Control of quality and silo storage of sunflower seeds using near infrared technology

    International Nuclear Information System (INIS)

    Gonzalez-Martin, I.; Vilaescusa-Garcia, V.; Lopez-Gonzalez, F.; Oiz-Jimenez, C.; Lobos-Ortega, I. A.; Gordillo, B.; Hernandez-Hierro, J. M.

    2013-01-01

    This work assesses the application of near infrared spectroscopy technology for the quality control of sunflower seeds direct from farmers and from a storage silo. The results show that the analytical method employing near infrared spectroscopy can be used as a rapid and non-destructive tool for the determination of moisture, fat and high/low oleic acid contents in samples of sunflower seeds. The ranges obtained were comparable to those reported for classic chemical methods, and were between 4.6-21.4% for moisture; 38.4-49.6% for fat, and 60.0-93.1% for oleic acid expressed as percentage of total fatty acids. A stepwise discriminant analysis was performed to determine the most useful wavelengths for classifying sunflower seeds in terms of their (high/low) oleic acid composition. The discriminant model allows the acid contents, with a prediction rate of 90.5% for internal validation and of 89.4% for cross-validation. (Author) 23 refs.

  1. Detailed studies on some lipids of Silybum marianum(L. seed oil

    Directory of Open Access Journals (Sweden)

    Hassanein, Minar M.

    2003-12-01

    Full Text Available Eight lipid patterns of Silybum marianum (L. Gaertn seed oil, not hitherto reported, (20% have been elucidated in this laboratory using capillary GLC and HPLC as main tools of analysis. The oil is rich in linoleic acid (53.3% and oleic acid (21.3%. Five major triacylglycerols containing linoleic acyls namely LLL, LLO, LLP, LOO and LOP were detected by HPLC using FID detector, these triacylglycerols are also predominating in both cottonseed and sunflower oils but in different proportions. The total tocopherols content (260 ppm was determined directly in the oil by HPLC. It was found that the oil contains alpha -tocopherol as a major constituent (84.5% resembling sunflower oil. The whole sterols pattern, as determined as their trimethylsilyl derivatives by GLC, includes campesterol, 5-stigmasterol, beta-sitosterol, 7-stigmasterol, avenasterol and spinasterol. The four sterols lipid clases of free and acylated sterols and sterylglycosides have been determined as their 9- and 1- anthroylnitrile derivatives by fluorescence and UV- HPLC respectively.La composición de ocho tipos de lípidos en aceite de semillas de Silybum marianum (L. Gaertn, hasta ahora no reportados, han sido identificados en este laboratorio, usando GLC capilar y HPLC como herramientas principales de análisis. El aceite es rico en ácidos linoleico (53,3% y oleico (21,3%. El ácido linoleico está contenido principalmente en cinco formas de triacilgliceroles: LLL, LLO, LLP, LOO y LOP, detectadas por HPLC, usando un detector FID, estos triacilgliceroles son también predominantes en los aceites de semillas de algodón o girasol, pero con diferentes proporciones. El contenido total de tocoferoles (260 ppm fue determinado directamente en el aceite por HPLC. Se encontró que el aceite contenía alfa-tocoferol como principal constituyente (84,5%, recordando al aceite de girasol. El patrón completo de esteroles determinado por GLC como derivados de trimetilsilil incluyó campesterol

  2. Chemical composition of Sacha Inchi (Plukenetia volubilis L. seeds and characteristics of their lipid fraction

    Directory of Open Access Journals (Sweden)

    Jiménez, Álvaro

    2011-03-01

    Full Text Available The seeds of Sacha inchi (Plukenetia volubilis L. from Colombia were analyzed for their main chemical composition. Sacha inchi seeds (SIS were rich in oil (41.4% and protein (24.7%. The main minerals present in SIS were potassium (5563.5 ppm, magnesium (3210 ppm and calcium (2406 ppm. A fatty acid analysis revealed that a-linolenic (50.8% and linoleic (33.4% acids were the main fatty acids in Sacha inchi oil (SIO. The lipid fractionation of SIO, obtained by solid phase extraction, yielded mainly neutral lipids (97.2%, and lower amounts of free fatty acids (1.2% and phospholipids (0.8%. The physicochemical properties of the oil include: saponification number 185.2; iodine value 193.1; density 0.9187 g/cm3, refractive index 1.4791 and viscosity of 35.4 mPa.s. The melting profiles of SIO were characterized by the presence of one wide endothermic peak with a melting enthalpy of 23.2 J/g. Our results indicate that Sacha inchi is an important new crop with applications in the food and pharmaceutical industries.Semillas de Sacha inchi (Plukenetia volubilis L. de Colombia fueron analizadas en su composición química. Las semillas de Sacha inchi (SIO fueron ricas en aceite (41.4% y proteína (24.7%. Los principales minerales presentes en las SIS fueron potasio (5563.5 ppm, magnesio (3210 ppm y calcio (2406 ppm. El análisis de ácidos grasos reveló que los ácidos a-linolénico (50.8% y linoleico (33.4% fueron los principales ácidos grasos presentes en el aceite de Sacha inchi (SIO. El fraccionamiento del SIO, realizado por extracción en fase sólida, produjo principalmente lípidos neutros (97.2%, y bajas cantidades de ácidos grasos libres (1.2% y fosfolípidos (0.8%. Las propiedades fisicoquímicas del aceite incluyen: índice de saponificación 185.2; índice de yodo 193.1; densidad 0.9187 g/cm3, índice de refracción 1.4791 y viscosidad 35.4 mPa.s. Los perfiles de fusión del SIO se caracterizaron por la presencia de un ancho pico endot

  3. Evaluation of Antioxidant Effects of Water and Ethanolic Extracts of Iranian Pomegranate Seed on Lipid Quality of Trout Fillet and Determining the Level of Perishability at 2-4 ° C

    Directory of Open Access Journals (Sweden)

    Gholam Reza Jahed Khaniky

    2015-08-01

    Full Text Available Background and Aims Because of unsaturated fatty acid content, trout and fish products are highly perishable. For this reason, preservatives are used to prevent or delay spoiling during storage. The aim of this study was determination of antioxidant activities of pomegranate seeds extracts on lipid quality of trout fillet stored at 2-4°C. Materials and Methods Prepared fish fillets were divided into two groups. One group was trout fillet without extract (control and one in pomegranate seeds extracts that were stored in refrigerator for 6 days. Chemical tests such as Peroxide Value (PV, Thiobarbituric Acid Reactive Substances (TBARS, Total Volatile Basic Nitrogen (TVB-N and Free Fatty Acids (FFA were used to determine perishability of trout fillet. Results The PV, TBA, TVB-N and FFA were significantly (P < 0.05 lowest in the samples treated with ethanolic extracts of pomegranate seeds as compared to control group. (PV = 2.62 meq o2/kg, TBA = 1.26 mg MDA/kg, TVB-N = 22.13 mg/100g and FFA = 1.2 percent Oleic acid. Conclusion The result of this study demonstrated that pomegranate seeds extracts were efficiency to prevent lipid oxidation in trout fillet stored at refrigerator temperature. * Corresponding Author: Tehran University of Medical Sciences, School of Public Health. Email: Salehia15@gmail.com

  4. Phisiological and biochemical characteristics of protein and lipid exchanges of maple and chestnut seeds from different regions of Dnepropetrovsk city technogenic pollution

    Directory of Open Access Journals (Sweden)

    I. O. Filonik

    2015-04-01

    Full Text Available The indexes of protein and lipid exchanges - the content of proteins, lipase activity, level of lipids and their composition, component composition of free fatty acids in the maple and chestnut seeds from several sites of Dnepropetrovsk technical pollution were investigated. The revealed figures can be used as biomarkers of anthropogenic pollution in industrial region.

  5. Effectivity of Sugar-Apple (Annona squamosa) Seed Extract with a Different Length of Storage against Culec quinquefasciatus Larvae

    OpenAIRE

    Heni Prasetyowati; Wisnu Satria

    2012-01-01

    Synthetic insecticide have been used to control Culex quinquefasciatus, but the prolonged usage of synthetic insecticide has a bad impact on the environment and may caused resistance. Sugar apple’s (Annona squamosa) seeds which contain alkaloid can be used as an alternative insecticide that was safe for environment. This research aims is to know the effect of sugar apple’s seeds with different length of storage as C. quinquefasciatus larvacide. This research was an experimental study with a r...

  6. Development of a Threshold Model to Predict Germination of Populus tomentosa Seeds after Harvest and Storage under Ambient Condition

    Science.gov (United States)

    Wang, Wei-Qing; Cheng, Hong-Yan; Song, Song-Quan

    2013-01-01

    Effects of temperature, storage time and their combination on germination of aspen (Populus tomentosa) seeds were investigated. Aspen seeds were germinated at 5 to 30°C at 5°C intervals after storage for a period of time under 28°C and 75% relative humidity. The effect of temperature on aspen seed germination could not be effectively described by the thermal time (TT) model, which underestimated the germination rate at 5°C and poorly predicted the time courses of germination at 10, 20, 25 and 30°C. A modified TT model (MTT) which assumed a two-phased linear relationship between germination rate and temperature was more accurate in predicting the germination rate and percentage and had a higher likelihood of being correct than the TT model. The maximum lifetime threshold (MLT) model accurately described the effect of storage time on seed germination across all the germination temperatures. An aging thermal time (ATT) model combining both the TT and MLT models was developed to describe the effect of both temperature and storage time on seed germination. When the ATT model was applied to germination data across all the temperatures and storage times, it produced a relatively poor fit. Adjusting the ATT model to separately fit germination data at low and high temperatures in the suboptimal range increased the models accuracy for predicting seed germination. Both the MLT and ATT models indicate that germination of aspen seeds have distinct physiological responses to temperature within a suboptimal range. PMID:23658654

  7. Identification of QTLs for seed germination capability after various storage periods using two RIL populations in rice.

    Science.gov (United States)

    Jiang, Wenzhu; Lee, Joohyun; Jin, Yong-Mei; Qiao, Yongli; Piao, Rihua; Jang, Sun Mi; Woo, Mi-Ok; Kwon, Soon-Wook; Liu, Xianhu; Pan, Hong-Yu; Du, Xinglin; Koh, Hee-Jong

    2011-04-01

    Seed germination capability of rice is one of the important traits in the production and storage of seeds. Quantitative trait loci (QTL) associated with seed germination capability in various storage periods was identified using two sets of recombinant inbred lines (RILs) which derived from crosses between Milyang 23 and Tong 88-7 (MT-RILs) and between Dasanbyeo and TR22183 (DT-RILs). A total of five and three main additive effects (QTLs) associated with seed germination capability were identified in MT-RILs and DT-RILs, respectively. Among them, six QTLs were identified repeatedly in various seed storage periods designated as qMT-SGC5.1, qMT-SGC7.2, and qMT-SGC9.1 on chromosomes 5, 7, and 9 in MT-RILs, and qDT-SGC2.1, qDT-SGC3.1, and qDT-SGC9.1 on chromosomes 2, 3, and 9 in DT-RILs, respectively. The QTL on chromosome 9 was identified in both RIL populations under all three storage periods, explaining up to 40% of the phenotypic variation. Eight and eighteen pairs additive × additive epistatic effect (epistatic QTL) were identified in MT-RILs and DT-RILs, respectively. In addition, several near isogenic lines (NILs) were developed to confirm six repeatable QTL effects using controlled deterioration test (CDT). The identified QTLs will be further studied to elucidate the mechanisms controlling seed germination capability, which have important implications for long-term seed storage.

  8. Effect of pre-plant treatments of yam (Dioscorea rotundata setts on the production of healthy seed yam, seed yam storage and consecutive ware tuber production

    Directory of Open Access Journals (Sweden)

    Abiodun Olufunmilayo Claudius-Cole

    2017-12-01

    Full Text Available Numerous pests and diseases of yams are perpetuated from season to season through the use of infected seed material. Developing a system for generating healthy seed material would disrupt this disease cycle and reduce losses in field and storage. The use of various pre-plant treatments was evaluated in field experiments carried out at three sites in Nigeria. Yam tubers of four preferred local cultivars were cut into 100 g setts and treated with pesticide (fungicide + insecticide mixture, neem extract (1 : 5 w/v, hot water (20 min at 53 °C or wood ash (farmers practice and compared with untreated setts. Pesticide treated setts sprouted better than all other treatments and generally led to lower pest and disease damage of yam tubers. Pesticide treatment increased tuber yields over most treatments, depending on cultivar, but effectively doubled the production as compared to the control. Pesticide and hot water treated setts produced the healthiest seed yams, which had lower storage losses than tubers from other treatments. These pre-treated seed yams produced higher yields corresponding to 700 % potential gain compared to the farmers usual practice. Treatments had no obvious influence on virus incidence, although virus-symptomatic plants yielded significantly less than nonsymptomatic plants. This study demonstrated that pre-plant treatment of setts with pesticide is a simple and effective method that guarantees more, heavier and healthier seed yam tubers.

  9. Dietary effect of pomegranate seed oil rich in 9cis, 11trans, 13cis conjugated linolenic acid on lipid metabolism in obese, hyperlipidemic OLETF Rats

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    Cha Jae-Young

    2004-11-01

    Full Text Available Abstract Conjugated fatty acid, the general term of positional and geometric isomers of polyunsaturated fatty acids with conjugated double bonds, has attracted considerable attention because of its potentially beneficial biological effects. In the present study, dietary effect of pomegranate seed oil rich in punicic acid (9cis, 11trans, 13cis-conjugated linolenic acid; 9c, 11t, 13c-CLNA on lipid metabolism was investigated in obese, hyperlipidemic Otsuka Long-Evans Tokushima Fatty (OLETF rats. After 2 weeks feeding period, OLETF rats revealed obesity and hyperlipidemia compared with their progenitor LETO rats. Feeding of the diet supplemented with 9% safflower oil and 1% pomegranate seed oil (9c, 11t, 13c-CLNA diet did not affect abdominal white adipose tissue weights and serum lipid levels compared with the diet supplemented with 10% safflower oil (control diet in OLETF rats. However, the accumulated hepatic triacylglycerol was markedly decreased by 9c, 11t, 13c-CLNA diet in OLETF rats. Activities of hepatic enzymes related to fatty acid synthesis and fatty acid β-oxidation were not altered by 9c, 11t, 13c-CLNA diet. Levels of monounsaturated fatty acid (MUFA, major storage form of fatty acid, in serum triacylglycerol were markedly higher in obese, hyperlipidemic OLETF rats than in lean LETO rats. In addition, 9c, 11t, 13c-CLNA diet significantly decreased MUFA levels in OLETF rats. This is the first study showing that 9c, 11t, 13c-CLNA suppresses delta-9 desaturation in vivo, and we suggest that the alleviation of hepatic triacylglycerol accumulation by 9c, 11t, 13c-CLNA diet was, at least in part, attributable to the suppression of delta-9 desaturation in OLETF rats.

  10. Storage on maternal plants affects light and temperature on requirements during germination in two small seeded halophytes in the arabian deserts

    International Nuclear Information System (INIS)

    Ali, A.; Gairola, S.

    2015-01-01

    Seeds are either stored in a soil seed bank or retained on maternal plants until they are released (aerial seed bank). Though there are extensive studies on the germination requirements of seeds in soil banks of saline habitats, studies conducted for halophytes with aerial seed banks are rare. We assessed the impact of aerial and room-temperature storages on the light and temperature requirements during germination in two small-seeded halophytes: Halocnmum strobilaceum having a short-term aerial seed bank (less than one year) and Halopeplis perfoliata having a longer term aerial seed bank (up to two years). Seed storage in the aerial bank reduced the germination in H. strobilaceum, but either increased it (5-months storage) or had no effect (17-months storage) in H. perfoliata. Seeds of both species that were stored in aerial bank germinated to higher percentages in light than in darkness, indicating that considerable portions of the seed populations are light sensitive. Seeds of H. perfoliata attained less than 5.0 percentage germination in darkness at higher temperatures, compared to more than 90.0 percentage in light. The results support the hypothesis that the aerial seed bank is an adaptive strategy for survival in the saline habitats of the two species. (author)

  11. Radition mutagenesis in lavender. Part 2. Effect of heat shock, moisture and post radiation storage on lavender seed radiosensitivity

    Energy Technology Data Exchange (ETDEWEB)

    Raev, R.C. (Institute of the Rose, Essential Oil and Medicinal Plants, Kazanlyk (Bulgaria))

    1983-01-01

    The influence of three factors which increase radiation tolerance of lavender seeds and reduce the biological injuries with lethal effect in case of gamma-irradiation (Cs/sup 137/) was investigated. Irradiation at -65 deg C increased radiation tolerance and led to increased doses and higher mutagenic effect. Seeds with lowered moisture had higher radiosensitivity in comparison to these having 4.5-5 times more water. Post-radiation storage at 20-22 deg C without loss of moisture increased radiation injuries, which grew along with the prolongation of the period from seed irradiation to germination.

  12. Radiological and clinical characterization of the lysosomal storage disorders: non-lipid disorders.

    Science.gov (United States)

    Parker, E I; Xing, M; Moreno-De-Luca, A; Harmouche, E; Terk, M R

    2014-01-01

    Lysosomal storage diseases (LSDs) are a large group of genetic metabolic disorders that result in the accumulation of abnormal material, such as mucopolysaccharides, glycoproteins, amino acids and lipids, within cells. Since many LSDs manifest during infancy or early childhood, with potentially devastating consequences if left untreated, timely identification is imperative to prevent irreversible damage and early death. In this review, the key imaging features of the non-lipid or extralipid LSDs are examined and correlated with salient clinical manifestations and genetic information. Disorders are stratified based on the type of excess material causing tissue or organ dysfunction, with descriptions of the mucopolysaccharidoses, mucolipidoses, alpha-mannosidosis, glycogen storage disorder II and cystinosis. In addition, similarities and differences in radiological findings between each of these LSDs are highlighted to facilitate further recognition. Given the rare and extensive nature of the LSDs, mastery of their multiple clinical and radiological traits may seem challenging. However, an understanding of the distinguishing imaging characteristics of LSDs and their clinical correlates may allow radiologists to play a key role in the early diagnosis of these progressive and potentially fatal disorders.

  13. Astaxanthin degradation and lipid oxidation of Pacific white shrimp oil: kinetics study and stability as affected by storage conditions

    Directory of Open Access Journals (Sweden)

    Sirima Takeungwongtrakul

    2016-02-01

    Full Text Available Abstract The kinetics of astaxanthin degradation and lipid oxidation in shrimp oil from hepatopancreas of Pacific white shrimp (Litopenaeus vannamei as affected by storage temperature were studied. When shrimp oil was incubated at different temperatures (4, 30, 45 and 60 °C for 16 h, the rate constants (k of astaxanthin degradation and lipid oxidation in shrimp oil increased with increasing temperatures (p < 0.05. Thus, astaxanthin degradation and lipid oxidation in shrimp oil were augmented at high temperature. When shrimp oils with different storage conditions (illumination, oxygen availability and temperature were stored for up to 40 days, astaxanthin contents in all samples decreased throughout storage (p < 0.05. All factors were able to enhance astaxanthin degradation during 40 days of storage. With increasing storage time, the progressive formation of primary and secondary oxidation products were found in all samples as evidenced by the increases in both peroxide values (PV and thiobarbituric acid reactive substances (TBARS (p < 0.05. Light, air and temperatures therefore had the marked effect on astaxanthin degradation and lipid oxidation in shrimp oils during the extended storage.

  14. POPPY SEED (PAPAVER SOMNIFERUM L.: EFFECT OF GENOTYPE AND YEAR OF CULTIVATION ON VARIABILITY IN ITS LIPID COMPOSITION

    Directory of Open Access Journals (Sweden)

    Michaela Havrlentová

    2012-02-01

    Full Text Available Poppy seeds have a high nutritive value and are used as a food and a source of edible oil. This oil is a rich source of polyunsaturated fatty acids. It is known that polyunsaturated fatty acids present not only basic nutriments for human body, but its taking to the organism is very important in term of protection against cardiovascular diseases, heart attacks and many inflammatory diseases. The goal of the study was to determine lipid content and fatty acids composition in eight selected poppy genotypes grown in experimental fields of the Plant Production Research Centre Piešťany – Research and Breeding Station at Malý Šariš (Slovak Republic in two years. Seed oils were analyzed by gas chromatography (GC-FID from prepared methylesters of fatty acids. The highest lipid content in 2007 was detected for genotype Opál (49.9%. In 2009, genotype ZB-6 contained the highest lipid content (50.1%. Linoleic acid was dominant fatty acid in all analyzed poppy oils. Its highest level contained the genotype ZB-5 (68.1% in 2007 and ZB-1 (66.5% in 2009. Other major fatty acids were palmitic and oleic acids. As minority fatty acids were presented stearic, alpha-linolenic and palmitoleic acids. Myristic, arachidic and gadoleic acids were observed in trace amounts. Furthermore, the effect of year of cultivation on the fatty acids content in poppy seed oils was examined by Student t-test and appropriate non-parametric Mann-Whitney test.

  15. Lack of Population Structure in Coriander Populations Based on SDS (Seed Storage Protein Page Analysis

    Directory of Open Access Journals (Sweden)

    Gülsüm Yaldiz

    2016-08-01

    Full Text Available Genetic variation is prerequisite for plant breeding. Nothing information existed in the literature for available diversity of Coriander accession in Turkey. Plant breeding activities are negligible in Turkey. So in order to start effective plant breeding program in Turkey, information on the available genetic diversity is viable. Therefore we planned to study the genetic variation and population structure of 29 Coriander accessions by seed storage protein (SDS. SDS analysis elaborated the lack of population structure and genetic bottleneck in the Coriander accessions in Turkey. Based on the results of this study, it was clear that sampling strategy was not appropriate and plant introduction should be made from different sources and diverse genotypes should be used as parents to initialize the effective Turkish Coriander breeding program.

  16. Survival and death of seeds during liquid nitrogen storage: a case study on seeds with short lifespans.

    Science.gov (United States)

    Ballesteros, D; Pence, V C

    The low temperature of liquid nitrogen is assumed to stop ageing and preserve viability indefinitely, however there are few validating data sets. The use of seeds to test these assumptions is important because other cryopreserved systems lack quantitative measures of viability to allow comparisons among timed points. To evaluate survival of a collection of seeds with short lifespans stored 12-20 years in liquid nitrogen. Seeds from 11 species (26 accessions) were removed from cryostorage and evaluated for germination and normal growth. Germination of Plantago cordata and Betula spp. seeds did not decrease significantly during cryostorage. However, Populus deltoides and most Salix spp. accessions showed a significant decrease in germination, with further loss observed when P. deltoides seedlings were followed to the young plant stage. Seeds of initial low quality showed greater deterioration during cryostorage. Cryostorage maintained viability of Salix and Populus seeds longer than other temperatures. However, ageing was not completely stopped and seed longevity was shorter than that predicted for many other species. A high initial seed quality is important in order to obtain the maximum benefit of cryostorage.

  17. CHARACTERIZATION OF SEED STORAGE PROTEINS IN SOME IRANIAN DATE PALM CULTIVARS USING SDS-PAGE

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    Sayed Mohammad Reza Khoshroo

    2013-08-01

    Full Text Available The date palm (Phoenix dactylifera L. is most adapted tree to grow in desert areas. It has always been looked on as a key source of stability, survival and evolution of the oasis agro-system since it constitutes the basic features of the ecological pyramid in desert regions. Determining genetic variability and cultivars identification in date palm are two major important factors in breeding programs, characterization of germplasm, and conservation purposes. The genetic variation of seed proteins was assayed by SDS-PAGE for 9 cultivars in Shahdad region in Iran. A total of 16 alternative protein bands with different mobility rates were identified within a molecular weight range of 11 KDa to 350 KDa. Then, electrophorogram for each cultivar was scored, and Jaccard‘s Similarity Index was calculated. Relying on UPGMA and NJ methods, genetic diversity of cultivars was evaluated by constructing the dendrogram for protein bands. Moreover, genetic distance was calculated for all of the cultivars.  It is concluded that seed storage protein profiles could be useful markers in genetic diversity studies and classification of cultivars. The cultivars from Shahdad were well separated from each other. This might have been done due to their unique genetic build-up. The cluster analysis displayed five major classes. In order to precise this assumption, data were computed to perform a PCA. Cluster analysis and PCA demonstrated their validity in establishing genetic diversity. When PCA was studied, the previously described results about Jaccard Similarity Coefficient dendrogram were also visualized.

  18. Effect of postirradiation storage of seeds on the structural chromosomal mutations in chlorophyll mutants of sunflower (Helianthus annuus L)

    International Nuclear Information System (INIS)

    Sizova, L.I.

    1976-01-01

    The paper comprises the results of the investigation of the storage effect in irradiated seeds on the frequency and the spectrum of structural mutations of chromosomes in normal green plants and in spontaneous chlorophyll mutants of sunflower. The seeds of chlorophyll mutants lutescens and xantha and those of green plants stored for 3 years have been 60 Co gamma-irradiated with doses of 0.5; 1.0; 2.0; 3.0; 5.0 and 10 kr. After the irradiation the seeds have been stored for a year under conventional laboratory conditions. As a result of the post-irradiation storage of seeds for a year the decrease in the proportion of aberrant cells by 3 to 6% at the expense of cells with paired fragments has been observed in green plants. In chlorophyll mutants the storage of seeds after the irradiation results in the 9 to 37% increase of the proportion of aberrant cells at the expense of cells with single and paired bridges and with paired fragments. This gives evidence in favour of the assumption that in spontaneous chlorophyll mutants the processes of the post-irradiation recovery either fail altogether, or proceed at a very low level

  19. Effective moisture diffusivity and activation energy of rambutan seed under different drying methods to promote storage stability

    Science.gov (United States)

    Ahmad, So'bah; Shamsul Anuar, Mohd; Saleena Taip, Farah; Shamsudin, Rosnah; M, Siti Roha A.

    2017-05-01

    The effects of two drying methods, oven and microwave drying on the effective moisture diffusivity and activation energy of rambutan seed were studied. Effective moisture diffusivity and activation energy are the main indicators used for moisture movement within the material. Hence, it is beneficial to determine an appropriate drying method to attain a final moisture content of rambutan seed that potentially could be used as secondary sources in the industry. An appropriate final moisture content will provide better storage stability that can extend the lifespan of the rambutan seed. The rambutan seeds were dried with two drying methods (oven and microwave) at two level of the process variables (oven temperature; 40°C and 60°C and microwave power; 250W and 1000W) at constant initial moisture contents. The result showed that a higher value of effective moisture diffusivity and less activation energy were observed in microwave drying compared to oven drying. This finding portrays microwave drying expedites the moisture removal to achieve the required final moisture content and the most appropriate drying method for longer storage stability for rambutan seed. With respect to the process variables; higher oven temperatures and lower microwave powers also exhibit similar trends. Hopefully, this study would provide a baseline data to determine an appropriate drying method for longer storage period for turning waste to by-products.

  20. The role of cold storage and seed source in the germination of three Mediterranean shrub species with contrasting dormancy types

    NARCIS (Netherlands)

    Vasques, Ana; Vallejo, V. Ramón; Santos, M. Conceição; Keizer, J. Jacob

    2014-01-01

    Context: The use of native species in ecological restoration is highly recommended but, in practice, it is often impaired by knowledge gaps in the germination ecology of suitable species. Aims: This study aimed to assess the role of storage conditions and seed source on the germination of three

  1. Effect of temperature towards lipid oxidation and non-enzymatic browning reactions in krill oil upon storage

    DEFF Research Database (Denmark)

    Lu, Henna Fung Sieng; Bruheim, I.; Haugsgjerd, B.O.

    2014-01-01

    was assessed by peroxide value and anisidine value, measurement of lipid derived volatiles, lipid classes and antioxidants. The non-enzymatic browning reactions were assessed through the measurement of pyrroles, free amino acids content and Strecker-derived volatiles. The increase of incubation temperature......The main objective of this study was to investigate the effect of temperature towards lipid oxidation and non-enzymatic browning reactions in krill oil upon storage. Krill oil was incubated at two different temperatures (20 and 40°C) for 28 or 42 days. The oxidative stability of krill oil...

  2. Proteomics for exploiting diversity of lupin seed storage proteins and their use as nutraceuticals for health and welfare.

    Science.gov (United States)

    Cabello-Hurtado, Francisco; Keller, Jean; Ley, José; Sanchez-Lucas, Rosa; Jorrín-Novo, Jesús V; Aïnouche, Abdelkader

    2016-06-30

    Lupins have a variety of both traditional and modern uses. In the last decade, reports assessing the benefits of lupin seed proteins have proliferated and, nowadays, the pharmaceutical industry is interested in lupin proteins for human health. Modern genomics and proteomics have hugely contributed to describing the diversity of lupin storage genes and, above all, proteins. Most of these studies have been centered on few edible lupin species. However, Lupinus genus comprises hundreds of species spread throughout the Old and New Worlds, and these resources have been scarcely explored and exploited. We present here a detailed review of the literature on the potential of lupin seed proteins as nutraceuticals, and the use of -omic tools to analyze seed storage polypeptides in main edible lupins and their diversity at the Lupinus inter- and intra-species level. In this sense, proteomics, more than any other, has been a key approach. Proteomics has shown that lupin seed protein diversity, where post-translational modifications yield a large number of peptide variants with a potential concern in bioactivity, goes far beyond gene diversity. The future extended use of second and third generation proteomics should definitely help to go deeper into coverage and characterization of lupin seed proteome. Some important topics concerning storage proteins from lupin seeds are presented and analyzed in an integrated way in this review. Proteomic approaches have been essential in characterizing lupin seed protein diversity, which goes far beyond gene diversity since the protein level adds to the latter differential proteolytic cleavage of conglutin pro-proteins and a diverse array of glycosylation forms and sites. Proteomics has also proved helpful for screening and studying Lupinus germplasm with the future aim of exploiting and improving food production, quality, and nutritional values. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. Lipids in hepatic glycogen storage diseases: pathophysiology, monitoring of dietary management and future directions.

    Science.gov (United States)

    Derks, Terry G J; van Rijn, Margreet

    2015-05-01

    Hepatic glycogen storage diseases (GSD) underscore the intimate relationship between carbohydrate and lipid metabolism. The hyperlipidemias in hepatic GSD reflect perturbed intracellular metabolism, providing biomarkers in blood to monitor dietary management. In different types of GSD, hyperlipidemias are of a different origin. Hypertriglyceridemia is most prominent in GSD type Ia and associated with long-term outcome morbidity, like pancreatitis and hepatic adenomas. In the ketotic subtypes of GSD, hypertriglyceridemia reflects the age-dependent fasting intolerance, secondary lipolysis and increased mitochondrial fatty acid oxidation. The role of high protein diets is established for ketotic types of GSD, but non-traditional dietary interventions (like medium-chain triglycerides and the ketogenic diet) in hepatic GSD are still controversial and necessitate further studies. Patients with these rare inherited disorders of carbohydrate metabolism meet several criteria of the metabolic syndrome, therefore close monitoring for cardiovascular diseases in ageing GSD patients may be justified.

  4. Effect of temperature and duration of maize pollen storage on the seed set rate

    Directory of Open Access Journals (Sweden)

    Babić Vojka

    2017-01-01

    Full Text Available In plant breeding programs, it is often necessary to cross genotypes incompatible in time of flowering. In maize, when the incompatibility in flowering period could not be overcome by different sowing dates, or by the ability of silk to preserve its fertility in 10-15 days, conservation of pollen could be of great importance. For many plant species, the appropriate methods for pollen management have been set up, including methods of collecting, desiccation, testing of viability and longevity, as well as for pollen storage. The longevity of pollen during its storage depends upon plant species, conditions at the time of pollen collecting, pollen moisture content, as well as upon storage temperature and duration. Even within the same plant species, different genotypes exhibit different level of viability preservation during the conservation. Although maize pollen belongs to a tricellular pollen group and rapidly loses viability under field conditions, its preservation is possible. According to the literature, pollen viability could be preserved for 30 days in the conditions of refrigerator (+4°C, while in liquid nitrogen (-196°C up to 120 days. In the majority of studies, pollen viability was evaluated in the laboratory conditions, and the results obtained could largely be differed from those obtained under field conditions, due to impossibility to control a number of environmental factors. This experiment was conducted under field conditions in order to evaluate the ability of pollen from commercial maize inbred line L217, stored under the conditions of +4°C and -20°C, to pollinate maize inbred L73B013 and produce grain. Inbred L73B013 is sown in five sowing dates in order to ensure the longer presence of fresh silk. Pollen samples from line L217 were taken twice, and along with silica gel, stored in refrigerator and freezer. Each of successive pollination included five silks. Except for the expected good ear seed set when silks were

  5. Tree seed handling, processing, testing, and storage at Hayward State Nursery, Wisconsin Department of Natural Resources

    Science.gov (United States)

    Gordon Christians

    2008-01-01

    The Hayward State Nursery, Wisconsin grows more than 40 species from seeds. Up to 6000 bushels of raw unprocessed tree and shrub seeds are collected each year, and all seeds are collected in Wisconsin or adjacent states. All white spruce (Picea glauca) and some white pine seeds (Pinus strobus) are collected from orchards containing...

  6. Gamma irradiation prolongs the sea bass (Dicentrarchus labrax L) storage and delays the lipids membrane degradation

    International Nuclear Information System (INIS)

    Barkallah, Insaf; Mahjoub, Abdelmajid; Cheour, F.

    2005-01-01

    Sea bass have been submitted to gamma rays radiation at doses 0, 1, 2, 3 or 4 KGy at 0,087KGY/min rate and stored during 21 days at 1 0 C to evaluate the effects on the development of microorganisms as well as on the conservation and the degradation of membranes lipids Microbiological analysis has revealed only the presence of mesophils germs in muscles which are totally eliminated at dose 4 KGy. The presence of psychrophiles, halophiles, coliformes, fecaux and totals, Staphylococcus aureus and Clostridium sulfutoreducteurs has been noted in the visceres of sea bass. They are completely eliminated at dose 1KGy. Contents in nitrogen basic fowl (ABVT) and in trimethylamine (TMA), as well as the pH increase during storage of fish in relation with the reduction of phospholipids, the diminution of the degree of insaturation of the phospholipids and the free fat acidic fraction, and the increase of the report sterols on phospholipids. The irradiation to gamma rays allows to slow these changes and consequently reduced the deterioration of the sea bass. We conclude that the irradiation to gamma rays could have prolong the conservation of sea bass sea bass by preserving probably lipids membranes of the degradation and by inhibiting the development of microorganisms

  7. Secagem e armazenamento de sementes de juçara Drying and storage of euterpe edulis seeds

    Directory of Open Access Journals (Sweden)

    Cibele Chalita Martins

    2009-08-01

    Full Text Available Com o objetivo de verificar o efeito da secagem parcial e do armazenamento sobre a germinação e vigor de sementes de Euterpe edulis obtidas em três épocas de produção, o lote 1 foi colhido em 10 plantasmatriz (PM, em 02/99; o lote 2 em 15 PM, em 04/00; e o lote 3 em 11 PM, em 08/00. Os frutos foram despolpados e as sementes, colocadas para secar por zero, 20 e 40 h (três sublotes, em câmara seca (temperatura de 27 ºC e umidade relativa de 35%. Os três sublotes foram armazenados a 10 ºC, em sacos plásticos (20 mm de espessura fechados. Durante o armazenamento, a qualidade das sementes foi avaliada a cada seis semanas, por 30 semanas, por meio das seguintes determinações: teor de água (105±3 ºC/24 h, porcentagem de germinação e vigor (primeira contagem e índice de velocidade de germinação. O aumento do tempo de secagem resultou em sementes com teor de água decrescente, em torno de 14 a 21% dos valores iniciais, que se mantiveram praticamente inalterados durante o armazenamento. A germinação e vigor das sementes foram prejudicados pela secagem parcial e pelo aumento do tempo de armazenamento, de forma diferenciada entre as épocas de produção, e ambas as características dependem das condições climáticas vigentes durante o desenvolvimento e maturação das sementes.The objective of this research was to verify the effects of partial drying and storage duration on the germination and vigor of three E. edulis seed lots developed under different weather conditions. Mature fruits were harvested in the palm collection from the Instituto Agronômico, located in Ubatuba, SP., forming three seed lots. Seeds from ten plants were harvested on 02/99 and constituted the Seed lot 1; seed lot 2 was composed of seeds of fifteen plants collected in 04/00, whereas seeds from eleven plants harvested on 08/00 composed seed lot 3. The fruits were depulped and the seeds were dried for 0, 20 and 40 hours, using a drying chamber regulated at

  8. Effects of pomegranate seed oil followed by resistance exercise on insulin resistance and lipid profile in non-athletic men

    Directory of Open Access Journals (Sweden)

    Fereshteh Shahidi

    2017-08-01

    Full Text Available Background: Although some studies have reported the health-related benefits for the pomegranate seed oil (PSO, there is not enough information on its combined effect with exercise. Therefore, in this study the effect of supplementation with pomegranate seed oil followed by resistance exercise on insulin resistance and lipid profile was considered in non-athletes men. Materials and Methods: In this semi-experimental double-blind randomized study non-athletic male (n=14 were divided into two groups: Exercise+Supplementation (n=7 and Exercise +Placebo (n=7. Both groups performed resistance training for 4 weeks (3 sessions per week. The experimental group consumed 2 capsules of pomegranate seed oil (400 mg and the control group received 2 placebo capsules daily. Glucose, fasting insulin, total cholesterol, triglycerides, LDL-C, HDL-C, were measured at the beginning and end of the study. Insulin resistance was estimated using homeostasis formula (HOMA-IR. Results: While the average concentration of HDL-C in Supplement+Exercise group was significantly increased compared to pre-test, no significant increase was seen compared to Placebo + Exercise group (P<0.05. Between and within group comparison for the changes in total cholesterol, triglycerides, LDL-C, glucose, fasting insulin and insulin resistance was not significant. Conclusion: According to the results, it can be concluded that 4 weeks of resistance training followed by PSO supplementation, except for HDL-C, has no significant effect on the other lipid profiles and insulin resistance in healthy non-athlete men.

  9. Terminalia arjuna: A novel natural preservative for improved lipid oxidative stability and storage quality of muscle foods

    Directory of Open Access Journals (Sweden)

    Insha Kousar Kalem

    2017-12-01

    Full Text Available The study was conducted to explore the possibility of utilization of Terminalia arjuna as a novel natural preservative in meat products by using chevon sausages as a model system. Chevon sausages were prepared by incorporating different levels of T. arjuna viz. T1 (0.25%, T2 (0.50% and T3 (0.75% and were assessed for various lipid oxidative stability and storage quality parameters under refrigerated (4 ± 1 °C conditions. T. arjuna showed a significant (p < 0.05 effect on the lipid oxidative stability as the treated products exhibited significantly (p < 0.05 lower TBARS (mg malonaldehyde/kg values in comparison to control. A significant (p < 0.05 effect was also observed on the microbial stability as T. arjuna incorporated products showed significantly (p < 0.05 lower values for total plate count (log cfu/g, psychrophilic count (log cfu/g, yeast and mould count (log cfu/g and FFA (% oleic acid values. Significantly (p < 0.05 higher scores were observed for various sensory parameters of the products incorporated with T. arjuna during refrigerated storage. T. arjuna successfully improved the lipid oxidative stability and storage quality of the model meat product and may be commercially exploited as a novel preservative in muscle foods. Keywords: Terminalia arjuna, Chevon sausages, Natural preservative, Lipid oxidation, Storage quality

  10. Armazenamento de sementes de carolina em diferentes temperaturas e embalagens Storage of carolina seeds in different temperature and packing

    Directory of Open Access Journals (Sweden)

    Camila de Oliveira

    2012-01-01

    Full Text Available O objetivo do presente trabalho foi determinar a embalagem e a temperatura adequadas para o armazenamento de sementes de carolina. As sementes foram armazenadas em sacos de papel e de plástico, mantidas em 0±2; 10±2; 20±2°C e 60 5% de umidade relativa do ar (UR e em temperatura ambiente (23,4±3,3°C e 68,7±9%UR. O teor de água, a germinação e o vigor foram determinados trimestralmente. Durante o armazenamento, o teor de água das sementes foi de aproximadamente 8,9%. A embalagem saco de plástico e a temperatura de 0°C são adequadas para o armazenamento das sementes de carolina.The objective of the present research was to determine the more adequated packing and temperature for storage of "carolina" seeds. The seeds were stored in paper and plastic packings and kept in 0±2; 10±2; 20±2°C and 60 5% air relative humidity (ARH and in ambient temperature (23,4±3,3°C and 68,7±9% ARH. Quarterly, the water seed content, germinative test and seed vigour were avaluated. During storage, the seed water content was approximately 8.9%. The plastic packing (plastic bag and tempertature of 0oC are adequated for storage of "carolina" seeds.

  11. Effect of squash seed meal (Cucurbita moschata on broiler performance, sensory meat quality, and blood lipid profile

    Directory of Open Access Journals (Sweden)

    YM Aguilar

    2011-12-01

    Full Text Available In this experiment, 240 Cobb-500® broilers reared from1 to 49 days, and distributed according to a completely experimental randomized design with four treatments of four replicates each in order to evaluate the effect of the dietary inclusion of 0, 33, 66 or 100 g/kg of squash seed meal (SSM (Cucurbita moschata on the performance, carcass yield, serum lipid profile and sensory meat quality of broilers. Significant differences (p<0.05 were detected in performance, carcass weight, weight and breast yield, and leg weight. The best results were obtained with 33 and 66 g/kg as compared to the control diet and 100 g SSM /kg. Abdominal fat decreased with the inclusion of 66 and 100 g SSM / kg, but the sensory quality of breast and thighs was not affected by the inclusion of SSM. The serum levels of total cholesterol, very low density (VLDL and low density (LDL lipoproteins, triglycerides, glucose and atherogenic index decreased with the inclusion of 100 g/kg of SSM, except for high density lipoproteins (HDL, which increased. The inclusion of 0, 33, 66 and 100 g/kg of SSM in broiler diets, partially replacing soybean meal and vegetable oil, improved live performance and edible portions yield. In addition, abdominal fat and serum levels of harmful lipids were reduced, whereas serum levels of beneficial lipids increased. There was no effect on meat sensory quality.

  12. The purification and characterization of a third storage protein (convicilin) from the seeds of pea (Pisum sativum L.).

    OpenAIRE

    Croy, R R; Gatehouse, J A; Tyler, M; Boulter, D

    1980-01-01

    A third storage protein, distinct from legumin and vicilin, has been purified from the seeds of pea (Pisum sativum L.). This protein has been named 'convicilin' and is present in protein bodies isolated from pea seeds. Convicilin has a subunit mol.wt. of 71 000 and a mol.wt. in its native form of 290 000. Convicilin is antigenically dissimilar to legumin, but gives a reaction of identity with vicilin when tested against antibodies raised against both proteins. However, convicilin contains no ...

  13. Impact of storage conditions on seed germination and seedling growth of wild oat (Avena fatua L. at different temperatures

    Directory of Open Access Journals (Sweden)

    Marija Sarić-Krsmanović

    2015-12-01

    Full Text Available The influence of seed storage conditions and different temperatures (5˚C, 10˚C, 15˚C, 20˚C, 25˚C, 30˚C and 26˚C/21˚C during germination and seedling development on seed germination, shoot length and germination rate of wild oat (Avena fatua L. was examined. Germinated seeds were counted daily over a period of ten days and shoot length was measured on the last day, while germination rates were calculated from those measurements. The results showed that seed storage under controlled conditions (T1: temperature 24±1°C, humidity 40-50%; T2: temperature 26±1°C, humidity 70-80% and T3: temperature 4˚C for periods of 3 (t1 and 12 (t2 months had a significant influence on germination of wild oat seeds. The percentage of germinated seeds under all examined temperatures was higher when they were stored for 12 months under controlled temperature and humidity. The results also showed that temperature had a significant effect on the percentage of germination and germination rate of A. fatua seeds. The highest total germination occurred at 15˚C temperature (T1: t1 - 41.25%, t2 - 44.37%; T2: t1 - 28.13%, t2 - 34.37%; T3: t1 - 10.63%, t2 - 12.50%. Germination percentage under an alternating day /night photoperiod at 26˚C/21˚C temperature was higher in all treatment variants (T1: t1 - 8.13%, t2 - 10.00%; T2: t1 - 11.87%, t2 - 13.13%; T3: t1 - 2.42%, t2 - 2.70% than germination in the dark at 25˚C, 30˚C and 5˚C.

  14. Biochemical Aspects of a Serine Protease from Caesalpinia echinata Lam. (Brazilwood Seeds: A Potential Tool to Access the Mobilization of Seed Storage Proteins

    Directory of Open Access Journals (Sweden)

    Priscila Praxedes-Garcia

    2012-01-01

    Full Text Available Several proteins have been isolated from seeds of leguminous, but this is the first report that a protease was obtained from seeds of Caesalpinia echinata Lam., a tree belonging to the Fabaceae family. This enzyme was purified to homogeneity by hydrophobic interaction and anion exchange chromatographies and gel filtration. This 61-kDa serine protease (CeSP hydrolyses H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide (Km 55.7 μM in an optimum pH of 7.1, and this activity is effectively retained until 50∘C. CeSP remained stable in the presence of kosmotropic anions (PO4 3−, SO4 2−, and CH3COO− or chaotropic cations (K+ and Na+. It is strongly inhibited by TLCK, a serine protease inhibitor, but not by E-64, EDTA or pepstatin A. The characteristics of the purified enzyme allowed us to classify it as a serine protease. The role of CeSP in the seeds cannot be assigned yet but is possible to infer that it is involved in the mobilization of seed storage proteins.

  15. Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp (Cyprinus carpio L. Liver

    Directory of Open Access Journals (Sweden)

    Elenka Georgieva

    2013-12-01

    Full Text Available We aimed in the present work is to study the effects of fosetyl-Al and fenamidone based fungicide (“Verita WG” on glycogen storage and expression of lipid droplets in common carp (Cyprinus carpio, L. liver. Concentrations of the test chemical were 30 mg/L, 38 mg/L and 50 mg/L under laboratory conditions. We used PAS-reaction for detection of glycogen storage and Sudan III staining for detection of lipid droplets in common carp hepatocytes. Hence, we found that the amount of glycogen and the fat storage in the liver increased proportionally with the increased fungicide concentrations. We also found conglomerates of accumulated glycogen in certain hepatocytes at all used concentrations. Overall, the results demonstrated enhanced glyconeogenesis and fat accumulation in the common carp liver, exposed to the test chemical.

  16. seeds

    African Journals Online (AJOL)

    Owner

    peptidohydrolase (8.0%) from mung bean seedlings. (Baumgartner and Chrispeels, 1977), EP-HG (4.5%) from horse gram seedlings ( Rajeswari, 1997), acidic protease (15%) from germinating winged-bean seeds. (Usha and Singh, 1996) and EP-1 (1.6%) from barley seedlings and GA3-induced cysteine protease (3.38%).

  17. Biochemical changes during aging of soybean seed

    Directory of Open Access Journals (Sweden)

    Balešević-Tubić Svetlana

    2009-01-01

    Full Text Available Biochemical changes that occur in the seed as a result of ageing are very significant for seed quality and longevity. Because of its characteristic composition, processes occurring in the seed of oil crops during storage will be typical as well. Six soybean varieties developed in Institute of field and vegetable crops Novi Sad, submitted to accelerated and natural aging, under controlled and conventional storage conditions were used in these trials. The content of malondialdehyde, superoxide dismutase and peroxidase activities were studied. The biochemical processes i.e. lipid peroxidation, as well as the decrease in supeoxide dismutase and peroxidase activities (especially pronounced by applied accelerated aging were caused by both type of aging. The degree of seed damage and the ability of seed to resist the negative consequences of aging were influenced, beside duration of aging period, by type of storage and characteristics of soybean varieties. .

  18. Innovative Techniques for Large-Scale Collection, Processing, and Storage of Eelgrass (Zostera marina) Seeds

    National Research Council Canada - National Science Library

    Orth, Robert J; Marion, Scott R

    2007-01-01

    .... Although methods for hand-collecting, processing and storing eelgrass seeds have advanced to match the scale of collections, the number of seeds collected has limited the scale of restoration efforts...

  19. Rapid screening for lipid storage disorders using biochemical markers. Expert center data and review of the literature.

    Science.gov (United States)

    Voorink-Moret, M; Goorden, S M I; van Kuilenburg, A B P; Wijburg, F A; Ghauharali-van der Vlugt, J M M; Beers-Stet, F S; Zoetekouw, A; Kulik, W; Hollak, C E M; Vaz, F M

    2018-02-01

    In patients suspected of a lipid storage disorder (sphingolipidoses, lipidoses), confirmation of the diagnosis relies predominantly on the measurement of specific enzymatic activities and genetic studies. New UPLC-MS/MS methods have been developed to measure lysosphingolipids and oxysterols, which, combined with chitotriosidase activity may represent a rapid first tier screening for lipid storage disorders. A lysosphingolipid panel consisting of lysoglobotriaosylceramide (LysoGb3), lysohexosylceramide (LysoHexCer: both lysoglucosylceramide and lysogalactosylceramide), lysosphingomyelin (LysoSM) and its carboxylated analogue lysosphingomyelin-509 (LysoSM-509) was measured in control subjects and plasma samples of predominantly untreated patients affected with lipid storage disorders (n=74). In addition, the oxysterols cholestane-3β,5α,6β-triol and 7-ketocholesterol were measured in a subset of these patients (n=36) as well as chitotriosidase activity (n=43). A systematic review of the literature was performed to assess the usefulness of these biochemical markers. Specific elevations of metabolites, i.e. without overlap between controls and other lipid storage disorders, were found for several lysosomal storage diseases: increased LysoSM levels in acid sphingomyelinase deficiency (Niemann-Pick disease type A/B), LysoGb3 levels in males with classical phenotype Fabry disease and LysoHexCer (i.e. lysoglucosylceramide/lysogalactosylceramide) in Gaucher and Krabbe diseases. While elevated levels of LysoSM-509 and cholestane-3β,5α,6β-triol did not discriminate between Niemann Pick disease type C and acid sphingomyelinase deficiency, LysoSM-509/LysoSM ratio was specifically elevated in Niemann-Pick disease type C. In Gaucher disease type I, mild increases in several lysosphingolipids were found including LysoGb3 with levels in the range of non-classical Fabry males and females. Chitotriosidase showed specific elevations in symptomatic Gaucher disease, and was mildly

  20. 11S Storage globulin from pumpkin seeds: regularities of proteolysis by papain.

    Science.gov (United States)

    Rudakova, A S; Rudakov, S V; Kakhovskaya, I A; Shutov, A D

    2014-08-01

    Limited proteolysis of the α- and β-chains and deep cleavage of the αβ-subunits by the cooperative (one-by-one) mechanism was observed in the course of papain hydrolysis of cucurbitin, an 11S storage globulin from seeds of the pumpkin Cucurbita maxima. An independent analysis of the kinetics of the limited and cooperative proteolyses revealed that the reaction occurs in two successive steps. In the first step, limited proteolysis consisting of detachments of short terminal peptides from the α- and β-chains was observed. The cooperative proteolysis, which occurs as a pseudo-first order reaction, started at the second step. Therefore, the limited proteolysis at the first step plays a regulatory role, impacting the rate of deep degradation of cucurbitin molecules by the cooperative mechanism. Structural alterations of cucurbitin induced by limited proteolysis are suggested to generate its susceptibility to cooperative proteolysis. These alterations are tentatively discussed on the basis of the tertiary structure of the cucurbitin subunit pdb|2EVX in comparison with previously obtained data on features of degradation of soybean 11S globulin hydrolyzed by papain.

  1. Hydrogen storage by carbon materials synthesized from oil seeds and fibrous plant materials

    Energy Technology Data Exchange (ETDEWEB)

    Sharon, Maheshwar; Bhardwaj, Sunil; Jaybhaye, Sandesh [Nanotechnology Research Center, Birla College, Kalyan 421304 (India); Soga, T.; Afre, Rakesh [Graduate School of Engineering, Nagoya Institute of Technology, Nagoya (Japan); Sathiyamoorthy, D.; Dasgupta, K. [Powder Metallurgy Division, BARC, Trombay 400 085 (India); Sharon, Madhuri [Monad Nanotech Pvt. Ltd., A702 Bhawani Tower, Powai, Mumbai 400 076 (India)

    2007-12-15

    Carbon materials of various morphologies have been synthesized by pyrolysis of various oil-seeds and plant's fibrous materials. These materials are characterized by SEM and Raman. Surface areas of these materials are determined by methylene blue method. These carbon porous materials are used for hydrogen storage. Carbon fibers with channel type structure are obtained from baggas and coconut fibers. It is reported that amongst the different plant based precursors studied, carbon from soyabean (1.09 wt%) and baggas (2.05 wt%) gave the better capacity to store hydrogen at 11kg/m{sup 2} pressure of hydrogen at room temperature. Efforts are made to correlate the hydrogen adsorption capacity with intensities and peak positions of G- and D-band obtained with carbon materials synthesized from plant based precursors. It is suggested that carbon materials whose G-band is around 1575cm{sup -1} and the intensity of D-band is less compared to G-band, may be useful material for hydrogen adsorption study. (author)

  2. Genetic diversity in radish germplasm for morphological traits and seed storage proteins

    International Nuclear Information System (INIS)

    Jatoi, S.A.; Siddiqui, S.U.; Masood, M.S.; Javaid, A.; Iqbal, M.; Sayal, O.U.

    2011-01-01

    Genetic variation of forty-nine local and exotic radish genotypes including two checks was studied for morphological traits and seed storage protein electrophoresis using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) markers. A high variation in germplasm for root shape, root length, root colour (internal and external), flesh texture and root type was observed. Among these genotypes, the genetic variation was apparent for most of the characters like plant biomass, root weight, leaf length, root length and root diameter that indicated the potential for crop improvement in these traits through simple selection. Exotic germplasm exhibited higher variation for plant biomass, root weight and root length which could be utilized through breeding programme. Cluster analysis on the basis of genetic diversity for seven quantitative traits resulted into four clusters. No clustering was found on the basis of origin. Low level of variance was observed for SDS-PAGE electrophoresis that suggested acquisition of more germplasm. On the basis of high yield and crispy root texture some genotypes (10076, 10362, 10429, 10658, 10662 and 10667) were identified for further testing under wide range of agro-ecological conditions. (author)

  3. Arabidopsis Intracellular NHX-Type Sodium-Proton Antiporters are Required for Seed Storage Protein Processing.

    Science.gov (United States)

    Ashnest, Joanne R; Huynh, Dung L; Dragwidge, Jonathan M; Ford, Brett A; Gendall, Anthony R

    2015-11-01

    The Arabidopsis intracellular sodium-proton exchanger (NHX) proteins AtNHX5 and AtNHX6 have a well-documented role in plant development, and have been used to improve salt tolerance in a variety of species. Despite evidence that intracellular NHX proteins are important in vacuolar trafficking, the mechanism of this role is poorly understood. Here we show that NHX5 and NHX6 are necessary for processing of the predominant seed storage proteins, and also influence the processing and activity of a vacuolar processing enzyme. Furthermore, we show by yeast two-hybrid and bimolecular fluorescence complementation (BiFC) technology that the C-terminal tail of NHX6 interacts with a component of Retromer, another component of the cell sorting machinery, and that this tail is critical for NHX6 activity. These findings demonstrate that NHX5 and NHX6 are important in processing and activity of vacuolar cargo, and suggest a mechanism by which NHX intracellular (IC)-II antiporters may be involved in subcellular trafficking. © The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  4. Genetic diversity of seed lipid content and fatty acid composition in ...

    African Journals Online (AJOL)

    Genetic diversity in seed oil content and fatty acid composition in six wild species of genus Sesamum viz., Sesamum mulayanum, Sesamum capense, Sesamum laciniatum, Sesamum latifolium, Sesamum occidentale and Sesamum schinzianum were studied and compared with the cultivated species Sesamum indicum or ...

  5. Optimum storage and germination conditions for seeds of pickerelweed (Pontetieria cordata L.) from Florida

    Science.gov (United States)

    Lyn A. Gettys; R. Kasten Dumroese

    2009-01-01

    Clean seeds of pickerelweed (Pontederia cordata L. [Pontederiaceae]) germinated best (84 to 94%) under water, even after being stored dry up to 6 mo at about 25 °C (77 °F), but germination of clean seeds under water was reduced to 43% when seeds were stored at 4 °C (39 3 F) for 6 mo. Underwater germination of seeds enclosed in fruits was less...

  6. The effect of storage temperature of steckling bulbs on seed stalk development and seed yield of shallot (Allium cepa L. var. ascalonicum Backer

    Directory of Open Access Journals (Sweden)

    Maria Tendaj

    2013-10-01

    Full Text Available The aim of this study, conducted in the years 2010–2012, was to evaluate bolting and seed production of shallot depending on storage temperature of steckling bulbs with different diameters. The present study included 4 cultivars (‘Toto’, ‘Ambition F1’, ‘Bonilla F1’, and ‘Matador F1’ and one local population (‘U’. Bulbs with the following diameters: 20–30 mm, 31–40 mm, 41–50 mm, and 51–60 mm, were stored from the first 10-day period of November (2010 and 2011 until the end of March (2011 and 2012 at a temperature of 0–1 oC, 4–6 oC, and 8–10 oC. After they were planted in the field (the second 10-day period of April, observations of bolting were carried out, while the weight of seed umbels and seed yield were determined only for the cultivar ‘Toto’ and the population ‘U’. The storage temperature of steckling bulbs in the range of 4–6 oC and 8–10 oC was most conducive to bolting, in particular in plants grown from large bulbs with a diameter above 40 mm. In the cultivar ‘Toto’, plants from bulbs with a diameter above 40 mm and stored at a temperature of 4–6 oC were characterized by the highest weight of seed umbels. This had an effect on obtaining the highest seed yield (on average 1604.16-2300.7 g per 100 m2 of area. Shallot plants from the population ‘U’ grown from bulbs with a diameter of 20–30 mm were characterized by a distinctly lower percentage of bolting plants compared to the cultivars studied. For this reason, this population does not promise positive effects in shallot production for seed.

  7. Lipid oxidation and vitamin D3 degradation in simulated whole milk powder as influenced by processing and storage.

    Science.gov (United States)

    Mahmoodani, Fatemeh; Perera, Conrad O; Abernethy, Grant; Fedrizzi, Bruno; Chen, Hong

    2018-09-30

    Vitamin D3 levels are known to sometimes decline in fortified products, which could be due to its degradation, although the exact mechanism is unknown. In this study, the influence of processing and storage conditions on lipid oxidation and vitamin D3 degradation were studied. Simulated whole milk powders with and without heat treatment were stored for 12 months at two different storage temperatures (20 °C and 40 °C). Stored samples without heat treatment showed higher lipid oxidation products analyzed by PV and TBARS values compared to those with heat treatment. Higher storage temperature also resulted in higher levels of lipid oxidation products. The concentration of vitamin D3 was also analyzed using UHPLC-MS/MS after PTAD derivatization in stored samples. An inverse relationship was observed between lipid oxidation products and vitamin D3 content. Finally, previtamin D3 and vitamin D3 oxidation products were quantified in stored samples using MRM analysis. Copyright © 2018 Elsevier Ltd. All rights reserved.

  8. Lipid oxidation, color changes and volatiles production in irradiated pork sausage with different fat content and packaging during storage

    International Nuclear Information System (INIS)

    Jo, C.; Lee, J.I.; Ahn, D.U.

    1999-01-01

    Effects of irradiation on lipid oxidation, color and volatiles production in pork sausages with different fat content and packaging were determined. Sausages (with 4.7, 10.5 and 15.8% fat content) were sliced and vacuum-packaged either in oxygen-permeable or impermeable bags, irradiated (0 or 4.5 kGy) and stored at 4°C for 7 days. Lipid oxidation, color and volatiles productions were analyzed at 0, 3 and 7 days of storage. TBARS (2-thiobarbituric acid reactive substances) values of cooked pork sausages increased with the increase of fat content regardless of storage, irradiation or packaging types. Irradiated samples had higher TBARS than nonirradiated at 0 day but the difference disappeared during storage in both packaging types. Lightness of sausages (Hunter L-value) increased with the increase of fat content and storage time but was not affected by irradiation. In aerobic packaging, irradiation reduced Hunter a-values of pork sausages at 0 day but irradiation effect on a-value disappeared during storage. In vacuum packaging, however, irradiated samples had higher Hunter a-values than nonirradiated samples. Irradiation increased 1-heptene and total volatiles, but the amount of 1-heptene was not associated well with TBARS values of pork sausages. In both irradiated and nonirradiated pork sausages, aerobic packaging produced more volatiles than vacuum packaging during storage. It was concluded that irradiation and fat content had significant effects on lipid oxidation, color and volatiles production of cooked pork sausages during storage but that oxygen availability had a stronger effect than irradiation and fat content

  9. Partial dehydration and cryopreservation of Citrus seeds.

    Science.gov (United States)

    Graiver, Natalia; Califano, Alicia; Zaritzky, Noemí

    2011-11-01

    Three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. Intermediate seeds cannot be stored in liquid nitrogen (LN) without a previous partial dehydration process. The water content (WC) of the seeds at the moment of immersion in LN must be regarded as the most critical factor in cryopreservation. The purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of Citrus seeds: C. sinensis (sweet orange), C. paradisi (grapefruit), C. reticulata (mandarin) in LN. To study the tolerance to dehydration and LN exposure, seeds were desiccated by equilibration at relative humidities between 11 and 95%. Sorption isotherms were determined and modeled; lipid content of the seeds was measured. Seed desiccation sensitivity was quantified by the quantal response model. Differential scanning calorimetry (DSC) thermograms were determined on cotyledon tissue at different moisture contents to measure ice melting enthalpies and unfrozen WC. Samples of total seed lipid extract were also analyzed by DSC to identify lipid transitions in the thermograms. The limit of hydration for LN Citrus seeds treatment corresponded to the unfrozen WC in the tissue, confirming that seed survival strictly depended on avoidance of intracellular ice formation. Copyright © 2011 Society of Chemical Industry.

  10. Effects of microwave cooking and refrigerated storage of main broiler parts on lipid oxidation in chicken muscle and skin

    International Nuclear Information System (INIS)

    Pikul, J.; Kummerow, F.A.

    1990-01-01

    From a total of 78 chickens, 24 carcasses were used to estimate the percentage for the individual cuts and their composition. Fifty-four carcasses were cut vertically into halves of which two-thirds were quartered, yielding front and hind quarters (Cuts 2 and 3). Half of these quarters were cut into individual pieces, yielding breasts and thighs with back ribs, drumsticks, and wings. The muscles and skin of one-third from each of the seven different cuts described above were analyzed raw for lipid oxidation products; while the remaining two-thirds were microwaved. Half of the microwaved cuts were analyzed 2 hours after cooking; the other half, after 4 days of storage at 4 C. The results indicated that the absolute amount of lipid oxidation products in chicken muscles and skin after microwave cooking and refrigerated storage was affected by the initial level of those products in the raw samples and by the particular cut of meat Cooking the different cuts of chicken carcasses by microwave significantly increased the amount of malonaldehyde (MA) and lipid-oxidation fluorescent products (LOFP) in the aqueous phase of Folch-extracted muscles and skin and in the organic phase of Folch-extracted skin lipids. Microwave cooking for the separate broiler parts (especially the drumsticks and wings, as compared to halves or quarters) produced the lowest amount of lipid oxidation products due to the shorter cooking time. Refrigerated storage of broiler parts cooked by microwave produced substantial amounts of MA and LOFP in the aqueous phase of the Folch extracted skin and in the organic phase of the Folch-extracted lipids from the muscles. (author)

  11. Diacylglycerol Enrichment of Endoplasmic Reticulum or Lipid Droplets Recruits Perilipin 3/TIP47 during Lipid Storage and Mobilization*

    Science.gov (United States)

    Skinner, James R.; Shew, Trevor M.; Schwartz, Danielle M.; Tzekov, Anatoly; Lepus, Christin M.; Abumrad, Nada A.; Wolins, Nathan E.

    2009-01-01

    Fatty acid-induced triacylglycerol synthesis produces triacylglycerol droplets with a protein coat that includes perilipin 3/TIP47 and perilipin 4/S3-12. This study addresses the following two questions. Where do lipid droplets emerge, and how are their coat proteins recruited? We show that perilipin 3- and perilipin 4-coated lipid droplets emerge along the endoplasmic reticulum (ER). Blocking membrane trafficking with AlF4− during fatty acid-induced triacylglycerol synthesis drove perilipin 3 to the tubular ER. Forskolin, which like AlF4− activates adenylate cyclase, did not redistribute perilipin 3, but when added together with AlF4− perilipin 3 was recruited to lipid droplets rather than the ER. Thus inhibiting trafficking with AlF4− redistributed perilipin 3 differently under conditions of triacylglycerol synthesis (fatty acid addition) versus hydrolysis (forskolin) suggesting a shared acylglycerol-mediated mechanism. We tested whether diacylglycerol (DG), the immediate precursor of triacylglycerol and its first hydrolytic product, affects the distribution of perilipin 3. Stabilizing DG with the DG lipase inhibitor RHC80267 enhanced the perilipin 3 recruited to lipid droplets and raised DG levels in this fraction. Treating cells with a membrane-permeable DG recruited perilipin 3 to the ER. Stabilizing DG, by blocking its hydrolysis with RHC80267 or its acylation with triacsin C, enhanced recruitment of perilipin 3 to the ER. Expressing the ER enzyme DGAT1, which removes DG by converting it to triacylglycerol, attenuated perilipin 3 DG-induced ER recruitment. Membrane-permeable DG also drove perilipin 4 and 5 onto the ER. Together the data suggest that these lipid droplet proteins are recruited to DG-enriched membranes thereby linking lipid coat proteins to the metabolic state of the cell. PMID:19748893

  12. Prospects of fatty acid profile and bioactive composition from lipid seeds for the discrimination of apple varieties with the application of chemometrics

    Energy Technology Data Exchange (ETDEWEB)

    Arain, S.; Sherazzi, S. T. H.; Bhanger, M. I.; Memon, N.; Mahesar, S. A.; Rajput, M. T.

    2012-11-01

    The extracted oils from four apple seed varieties (Royal Gala, Red Delicious, Pyrus Malus and Golden Delicious) from Pakistan were investigated for their fatty acid profiles and lipid biactives by GC-MS. The oil contents in the seeds of the apple varieties ranged from 26.8-28.7%. The results revealed that linoleic acid (40.5-49.6%) was the main fatty acid in the Royal Gala, Red Delicious and Pyrus Malus seeds, and oleic acid (38.7-45.5%) was the main fatty acid in the Golden Delicious seeds. Palmitic acid (6.1-7.4%) and stearic acid (2.0-3.1%) were the dominant saturated fatty acids, besides the small amount of palmitoleic, heptadecanoic, linolenic, archidic, eicosanoic, and behenic acids. Sterols, tocopherols, hydrocarbons and some other minor components were also identified from the unsaponifiable lipid fraction. The variation among the results of both fatty acids and lipid bio actives for the four different varieties was assessed by principal component analysis, discriminant analysis and cluster analyses. The results conclude that both oil fractions could be applied as a useful tool to discriminate among the apple seed varieties. (Author) 42 refs.

  13. Effectivity of Sugar-Apple (Annona squamosa Seed Extract with a Different Length of Storage against Culec quinquefasciatus Larvae

    Directory of Open Access Journals (Sweden)

    Heni Prasetyowati

    2012-06-01

    Full Text Available Synthetic insecticide have been used to control Culex quinquefasciatus, but the prolonged usage of synthetic insecticide has a bad impact on the environment and may caused resistance. Sugar apple’s (Annona squamosa seeds which contain alkaloid can be used as an alternative insecticide that was safe for environment. This research aims is to know the effect of sugar apple’s seeds with different length of storage as C. quinquefasciatus larvacide. This research was an experimental study with a randomized controlled trial group design approach. The test material was an extract of sugar apple’s seeds which have been kept for 0, 1, 2, and 3 week with LC50 (0,47 ppm was used. Each treatment used 25 C. quinquefasciatus larvae from third instar larvae stage and replicated five times. After exposed for 24 hours, dead larvae counted. The result confirmed that the extract of sugar apple’s seeds which has been stored in 0, 1, 2, and 3 week did not showed any significant different on larvae mortality. Extract of sugar apple’s seeds which have been stored in 0, 1, 2, and 3 week have an equal activity as Culex quinquefasciatus larvicide.

  14. Neutral lipid storage disease with myopathy: A whole-body nuclear MRI and metabolic study

    International Nuclear Information System (INIS)

    Laforet, Pascal; Stojkovic, Tanya; Wahbi, Karim; Eymard, Bruno; Bassez, Guillaume; Carlier, Pierre G.; Clement, Karine; Petit, Francois M.; Carlier, Robert-Yves

    2013-01-01

    Neutral lipid storage disease with myopathy (NLSDM) is caused by a mutation in the gene encoding adipose triglyceride lipase (ATGL), and is characterized by the presence of numerous triglyceride-containing cytoplasmic droplets in type I muscle fibers. Major clinical manifestations concern the heart and skeletal muscle, and some patients also present diabetes mellitus. We report the clinical, metabolic, and whole-body nuclear magnetic resonance imaging findings of three patients with NLSDM. Muscle MRI study was consistent with previous descriptions, and allowed to show a common pattern of fatty replacement. Muscle changes predominated in the paravertebral muscles, both compartments of legs, and posterior compartment of the thighs. A more variable distribution of muscle involvement was observed on upper limbs, with marked asymmetry in one patient, and alterations predominating on supra and infra spinatus, biceps brachialis and anterior compartment of arms. Cardiac NMR studies revealed anomalies despite normal echocardiography in two patients. Endocrine studies showed low leptin and adiponectine levels, a moderate increase in insulin levels at fasting state, and even greater increase after oral glucose tolerance test in one patient. Two patients had elevated triglycerides and low cholesterol-HDL. Based on these analyses, regular control of cardio-metabolic risks appear mandatory in the clinical follow-up of these subjects. (authors)

  15. Neutral lipid storage disease with myopathy: A whole-body nuclear MRI and metabolic study

    Energy Technology Data Exchange (ETDEWEB)

    Laforet, Pascal; Stojkovic, Tanya; Wahbi, Karim; Eymard, Bruno [AP-HP, Centre de Reference de pathologie neuromusculaire Paris-Est, Groupe Hospitalier Pitie-Salpetriere, Assistance Publique-Hopitaux de Paris, Paris, (France); Bassez, Guillaume [AP-HP, Centre de Reference de Pathologie Neuromusculaire Paris-Ouest, CHU Henri Mondor, Creteil, (France); Carlier, Pierre G. [CEA, I2BM, MIRCen, IdM NMR Laboratory, T-75651 Paris, (France); Clement, Karine [AP-HP, Institute of Cardiometabolism and Nutrition, ICAN, Pitie-Salpetriere Hospital, University Pierre et Marie-Curie Paris6, Paris, INSERM, U872 team 7, Paris, (France); Petit, Francois M. [AP-HP, Molecular Genetics and Metabolic Diseases Laboratory, Antoine Beclere Hospital, Clamart, (France); Carlier, Robert-Yves [AP-HP, Departement d' imagerie Medicale et Centre d' innovation Technologique, CHU Raymond-Poincare, Garches, (France)

    2013-07-01

    Neutral lipid storage disease with myopathy (NLSDM) is caused by a mutation in the gene encoding adipose triglyceride lipase (ATGL), and is characterized by the presence of numerous triglyceride-containing cytoplasmic droplets in type I muscle fibers. Major clinical manifestations concern the heart and skeletal muscle, and some patients also present diabetes mellitus. We report the clinical, metabolic, and whole-body nuclear magnetic resonance imaging findings of three patients with NLSDM. Muscle MRI study was consistent with previous descriptions, and allowed to show a common pattern of fatty replacement. Muscle changes predominated in the paravertebral muscles, both compartments of legs, and posterior compartment of the thighs. A more variable distribution of muscle involvement was observed on upper limbs, with marked asymmetry in one patient, and alterations predominating on supra and infra spinatus, biceps brachialis and anterior compartment of arms. Cardiac NMR studies revealed anomalies despite normal echocardiography in two patients. Endocrine studies showed low leptin and adiponectine levels, a moderate increase in insulin levels at fasting state, and even greater increase after oral glucose tolerance test in one patient. Two patients had elevated triglycerides and low cholesterol-HDL. Based on these analyses, regular control of cardio-metabolic risks appear mandatory in the clinical follow-up of these subjects. (authors)

  16. Impaired Autophagy in the Lipid-Storage Disorder Niemann-Pick Type C1 Disease

    Directory of Open Access Journals (Sweden)

    Sovan Sarkar

    2013-12-01

    Full Text Available Autophagy dysfunction has been implicated in misfolded protein accumulation and cellular toxicity in several diseases. Whether alterations in autophagy also contribute to the pathology of lipid-storage disorders is not clear. Here, we show defective autophagy in Niemann-Pick type C1 (NPC1 disease associated with cholesterol accumulation, where the maturation of autophagosomes is impaired because of defective amphisome formation caused by failure in SNARE machinery, whereas the lysosomal proteolytic function remains unaffected. Expression of functional NPC1 protein rescues this defect. Inhibition of autophagy also causes cholesterol accumulation. Compromised autophagy was seen in disease-affected organs of Npc1 mutant mice. Of potential therapeutic relevance is that HP-β-cyclodextrin, which is used for cholesterol-depletion treatment, impedes autophagy, whereas stimulating autophagy restores its function independent of amphisome formation. Our data suggest that a low dose of HP-β-cyclodextrin that does not perturb autophagy, coupled with an autophagy inducer, may provide a rational treatment strategy for NPC1 disease.

  17. Impaired autophagy in the lipid-storage disorder Niemann-Pick type C1 disease.

    Science.gov (United States)

    Sarkar, Sovan; Carroll, Bernadette; Buganim, Yosef; Maetzel, Dorothea; Ng, Alex H M; Cassady, John P; Cohen, Malkiel A; Chakraborty, Souvik; Wang, Haoyi; Spooner, Eric; Ploegh, Hidde; Gsponer, Joerg; Korolchuk, Viktor I; Jaenisch, Rudolf

    2013-12-12

    Autophagy dysfunction has been implicated in misfolded protein accumulation and cellular toxicity in several diseases. Whether alterations in autophagy also contribute to the pathology of lipid-storage disorders is not clear. Here, we show defective autophagy in Niemann-Pick type C1 (NPC1) disease associated with cholesterol accumulation, where the maturation of autophagosomes is impaired because of defective amphisome formation caused by failure in SNARE machinery, whereas the lysosomal proteolytic function remains unaffected. Expression of functional NPC1 protein rescues this defect. Inhibition of autophagy also causes cholesterol accumulation. Compromised autophagy was seen in disease-affected organs of Npc1 mutant mice. Of potential therapeutic relevance is that HP-β-cyclodextrin, which is used for cholesterol-depletion treatment, impedes autophagy, whereas stimulating autophagy restores its function independent of amphisome formation. Our data suggest that a low dose of HP-β-cyclodextrin that does not perturb autophagy, coupled with an autophagy inducer, may provide a rational treatment strategy for NPC1 disease. Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

  18. Extractions of High Quality RNA from the Seeds of Jerusalem Artichoke and Other Plant Species with High Levels of Starch and Lipid.

    Science.gov (United States)

    Mornkham, Tanupat; Wangsomnuk, Preeya Puangsomlee; Fu, Yong-Bi; Wangsomnuk, Pinich; Jogloy, Sanun; Patanothai, Aran

    2013-04-29

    Jerusalem artichoke (Helianthus tuberosus L.) is an important tuber crop. However, Jerusalem artichoke seeds contain high levels of starch and lipid, making the extraction of high-quality RNA extremely difficult and the gene expression analysis challenging. This study was aimed to improve existing methods for extracting total RNA from Jerusalem artichoke dry seeds and to assess the applicability of the improved method in other plant species. Five RNA extraction methods were evaluated on Jerusalem artichoke seeds and two were modified. One modified method with the significant improvement was applied to assay seeds of diverse Jerusalem artichoke accessions, sunflower, rice, maize, peanut and marigold. The effectiveness of the improved method to extract total RNA from seeds was assessed using qPCR analysis of four selected genes. The improved method of Ma and Yang (2011) yielded a maximum RNA solubility and removed most interfering substances. The improved protocol generated 29 to 41 µg RNA/30 mg fresh weight. An A260/A280 ratio of 1.79 to 2.22 showed their RNA purity. Extracted RNA was effective for downstream applications such as first-stranded cDNA synthesis, cDNA cloning and qPCR. The improved method was also effective to extract total RNA from seeds of sunflower, rice, maize and peanut that are rich in polyphenols, lipids and polysaccharides.

  19. Extractions of High Quality RNA from the Seeds of Jerusalem Artichoke and Other Plant Species with High Levels of Starch and Lipid

    Directory of Open Access Journals (Sweden)

    Tanupat Mornkham

    2013-04-01

    Full Text Available Jerusalem artichoke (Helianthus tuberosus L. is an important tuber crop. However, Jerusalem artichoke seeds contain high levels of starch and lipid, making the extraction of high-quality RNA extremely difficult and the gene expression analysis challenging. This study was aimed to improve existing methods for extracting total RNA from Jerusalem artichoke dry seeds and to assess the applicability of the improved method in other plant species. Five RNA extraction methods were evaluated on Jerusalem artichoke seeds and two were modified. One modified method with the significant improvement was applied to assay seeds of diverse Jerusalem artichoke accessions, sunflower, rice, maize, peanut and marigold. The effectiveness of the improved method to extract total RNA from seeds was assessed using qPCR analysis of four selected genes. The improved method of Ma and Yang (2011 yielded a maximum RNA solubility and removed most interfering substances. The improved protocol generated 29 to 41 µg RNA/30 mg fresh weight. An A260/A280 ratio of 1.79 to 2.22 showed their RNA purity. Extracted RNA was effective for downstream applications such as first-stranded cDNA synthesis, cDNA cloning and qPCR. The improved method was also effective to extract total RNA from seeds of sunflower, rice, maize and peanut that are rich in polyphenols, lipids and polysaccharides.

  20. Changes of microbial spoilage, lipid-protein oxidation and physicochemical properties during post mortem refrigerated storage of goat meat.

    Science.gov (United States)

    Sabow, Azad Behnan; Sazili, Awis Qurni; Aghwan, Zeiad Amjad; Zulkifli, Idrus; Goh, Yong Meng; Ab Kadir, Mohd Zainal Abidin; Nakyinsige, Khadijah; Kaka, Ubedullah; Adeyemi, Kazeem Dauda

    2016-06-01

    Examined was the effect of post mortem refrigerated storage on microbial spoilage, lipid-protein oxidation and physicochemical traits of goat meat. Seven Boer bucks were slaughtered, eviscerated and aged for 24 h. The Longissimus lumborum (LL) and Semitendinosus (ST) muscles were excised and subjected to 13 days post mortem refrigerated storage. The pH, lipid and protein oxidation, tenderness, color and drip loss were determined in LL while microbiological analysis was performed on ST. Bacterial counts generally increased with increasing aging time and the limit for fresh meat was reached at day 14 post mortem. Significant differences were observed in malondialdehyde (MDA) content at day 7 of storage. The thiol concentration significantly reduced as aging time increased. The band intensities of myosin heavy chain (MHC) and troponin-T significantly decreased as storage progressed, while actin remained relatively stable. After 14 days of aging, tenderness showed significant improvement while muscle pH and drip loss reduced with increase in storage time. Samples aged for 14 days had higher lightness (P goat meat. © 2016 Japanese Society of Animal Science.

  1. Effect of gamma irradiation on the lipid peroxidation in chicken, lamb and buffalo meat during chilled storage

    International Nuclear Information System (INIS)

    Kanatt, S.R.; Paul, P.; D'Souza, S.F.; Thomas, P.

    1997-01-01

    Chicken, lamb and buffalo meat were subjected to low-dose gamma irradiation (2.5 kGy) and stored at 0-3C. Lipid peroxidation in terms of thiobarbituric acid (TBA) number and carbonyl content were monitored during storage. While irradiated meat showed slight increase in TBA number and carbonyl content on storage as compared to nonirradiated meat, this did not affect the sensory qualities of meat. Free fatty acid content decreased markedly on irradiation. Irradiated meats were microbiologically safe and sensorily acceptable up to 4 weeks in the nonfrozen state (0-3C) while nonirradiated meat had a shelf-life of less than 2 weeks

  2. Lipid peroxidation in chicken meat during chilled storage as affected by antioxidants combined with low-dose gamma irradiation

    International Nuclear Information System (INIS)

    Kanatt, S.R.; Paul, P.; D'Souza, S.F.; Thomas, P.

    1998-01-01

    TBA values and carbonyl content for irradiated samples of ground chicken meat were higher than for nonirradiated samples. Addition of antioxidants tocopherol (natural) or BHT (synthetic) resulted in retardation of oxidative rancidity (p0.05). Meat treated with antioxidants prior to irradiation had lower TBA values as compared to untreated irradiated counterparts. Free fatty acid (FFA) values decreased after irradiation. Addition of antioxidants prior to irradiation showed a synergistic effect in decreasing FFA content. TLC of muscle lipids indicated a reduction in the triacylglcerols content with concomitant increases in FFA of all samples during storage. All irradiated meats were acceptable for consumption up to 4 wk of storage

  3. Comparison of the effects of flaxseed oil and sunflower seed oil consumption on serum glucose, lipid profile, blood pressure, and lipid peroxidation in patients with metabolic syndrome.

    Science.gov (United States)

    Akrami, Atefeh; Nikaein, Farzad; Babajafari, Siavash; Faghih, Shiva; Yarmohammadi, Hassan

    Metabolic syndrome (MetSyn) increases the risk of type II diabetes and morbidity and mortality due to cardiovascular diseases. Flaxseed oil (FO), as a functional food, is one of the major vegetal sources of essential omega-3 fatty acids. This study aimed to compare the effects of consumption of FO and sunflower seed oil (SO) on lipid peroxidation and other symptoms of MetSyn. This randomized controlled interventional trial was conducted on 60 volunteers aged 30 to 60 years who were diagnosed with MetSyn in Shiraz, Iran. The participants who fulfilled the inclusion criteria were randomly assigned to SO (n = 30, receiving 25 mL/d SO) and FO (n = 30, receiving 25 ml/d FO) groups using block randomization. The diets were identical for all the participants. Blood pressure (BP), serum lipid, fasting blood sugar, and malondialdehyde were measured at baseline and at the end of week 7. The results showed no significant difference between the 2 groups regarding blood lipid levels and fasting blood sugar at the end of the study. However, significant reductions in total cholesterol, low-density lipoprotein cholesterol (5.6% in FO and 10.8% in SO), and triglyceride levels were seen within each group after treatment with FO and SO (P < .05). Nonetheless, between-group changes were significant (<0.05) for systolic BP (mean [±standard deviation {SD}] changes were -14.0 ± 22.41 in the FO group [P = .004] and 0.92 ± 8.70 in the SO group [P = .594]) and diastolic BP (mean [±SD] changes were -4.26 ± 7.44 in the FO group [P = .007] and 1.30 ± 6.91 in the SO group [P = .344]), but marginally significant (P = .053) for malondialdehyde level (mean [±SD] changes were -1.29 ± 1.48 in the FO group [P < .001] and -0.52 ± 1.34 in the SO group [P = .52]). A significant decrease in weight was also found in both groups. However, waist circumference decreased significantly only in the FO group at the end of the study (P < .05). Our results indicated

  4. Effects of garlic extract on color, lipid oxidation and oxidative breakdown products in raw ground beef during refrigerated storage

    Directory of Open Access Journals (Sweden)

    XINZHUANG ZHANG

    2016-03-01

    Full Text Available The study aims to investigate the effects of garlic extracts on color, lipid oxidation, and oxidative breakdown products in raw ground beef during refrigerated storage. The two treatments were:control group (C, with no addition and experiment group (D, 50 mg garlic extracts added to 100 g beef. Adding garlic extracts significant increased a* value (PA ≤ 0.05, and significant decreased TBARS and PV values (PA ≤ 0.05. The pH and –SH value of D group had a decreasing tendency (PA=0.0522 and an increasing tendency (PA=0.0636 respectively compared to C group. Garlic extracts protected phospholipids, fatty acids and polypeptides from oxidation. The results indicatethat garlic extracts have the antioxidant activity, helping maintain the meat color, inhibiting lipid oxidation and protein degradation of raw ground beef during refrigerated storage.

  5. RNase L controls terminal adipocyte differentiation, lipids storage and insulin sensitivity via CHOP10 mRNA regulation

    DEFF Research Database (Denmark)

    Fabre, Odile Martine Julie; Salehzada, T; Lambert, K

    2012-01-01

    Adipose tissue structure is altered during obesity, leading to deregulation of whole-body metabolism. Its function depends on its structure, in particular adipocytes number and differentiation stage. To better understand the mechanisms regulating adipogenesis, we have investigated the role...... is associated with CHOP10 mRNA and regulates its stability. CHOP10 expression is conserved in RNase L(-/-)-MEFs, maintaining preadipocyte state while impairing their terminal differentiation. RNase L(-/-)-MEFs have decreased lipids storage capacity, insulin sensitivity and glucose uptake. Expression of ectopic...... RNase L in RNase L(-/-)-MEFs triggers CHOP10 mRNA instability, allowing increased lipids storage, insulin response and glucose uptake. Similarly, downregulation of CHOP10 mRNA with CHOP10 siRNA in RNase L(-/-)-MEFs improves their differentiation in adipocyte. In vivo, aged RNase L(-)/(-) mice present...

  6. Variation of germination of eggplant (Solanum melongena L.) seed during storage life

    OpenAIRE

    Ristić, Nevena; Todorović, Vesna; Adžić, Slađan; Zdravković, Jasmina

    2013-01-01

    Eggplant (Solanum melongena L.) seed is dormant, which means that the maximum germination is achieved after some time. The research was performed on one genotype (Domaci srednje dugi - DSD). The seed, studied for germination rate, originates from year 2007 and the germination variation was studied for period 2007 - 2011. Eggplant seed was dormant after yield in 2007, but the germination increased over the years. The trial was conducted at the standard germination test method, defined in the R...

  7. Lipid metabolic perturbation is an early-onset phenotype in adult spinster mutants: a Drosophila model for lysosomal storage disorders.

    Science.gov (United States)

    Hebbar, Sarita; Khandelwal, Avinash; Jayashree, R; Hindle, Samantha J; Chiang, Yin Ning; Yew, Joanne Y; Sweeney, Sean T; Schwudke, Dominik

    2017-12-15

    Intracellular accumulation of lipids and swollen dysfunctional lysosomes are linked to several neurodegenerative diseases, including lysosomal storage disorders (LSD). Detailed characterization of lipid metabolic changes in relation to the onset and progression of neurodegeneration is currently missing. We systematically analyzed lipid perturbations in spinster (spin) mutants, a Drosophila model of LSD-like neurodegeneration. Our results highlight an imbalance in brain ceramide and sphingosine in the early stages of neurodegeneration, preceding the accumulation of endomembranous structures, manifestation of altered behavior, and buildup of lipofuscin. Manipulating levels of ceramidase and altering these lipids in spin mutants allowed us to conclude that ceramide homeostasis is the driving force in disease progression and is integral to spin function in the adult nervous system. We identified 29 novel physical interaction partners of Spin and focused on the lipid carrier protein, Lipophorin (Lpp). A subset of Lpp and Spin colocalize in the brain and within organs specialized for lipid metabolism (fat bodies and oenocytes). Reduced Lpp protein was observed in spin mutant tissues. Finally, increased levels of lipid metabolites produced by oenocytes in spin mutants allude to a functional interaction between Spin and Lpp, underscoring the systemic nature of lipid perturbation in LSD. © 2017 Hebbar et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  8. Acyl-Lipid Metabolism

    Science.gov (United States)

    Li-Beisson, Yonghua; Shorrosh, Basil; Beisson, Fred; Andersson, Mats X.; Arondel, Vincent; Bates, Philip D.; Baud, Sébastien; Bird, David; DeBono, Allan; Durrett, Timothy P.; Franke, Rochus B.; Graham, Ian A.; Katayama, Kenta; Kelly, Amélie A.; Larson, Tony; Markham, Jonathan E.; Miquel, Martine; Molina, Isabel; Nishida, Ikuo; Rowland, Owen; Samuels, Lacey; Schmid, Katherine M.; Wada, Hajime; Welti, Ruth; Xu, Changcheng; Zallot, Rémi; Ohlrogge, John

    2013-01-01

    Acyl lipids in Arabidopsis and all other plants have a myriad of diverse functions. These include providing the core diffusion barrier of the membranes that separates cells and subcellular organelles. This function alone involves more than 10 membrane lipid classes, including the phospholipids, galactolipids, and sphingolipids, and within each class the variations in acyl chain composition expand the number of structures to several hundred possible molecular species. Acyl lipids in the form of triacylglycerol account for 35% of the weight of Arabidopsis seeds and represent their major form of carbon and energy storage. A layer of cutin and cuticular waxes that restricts the loss of water and provides protection from invasions by pathogens and other stresses covers the entire aerial surface of Arabidopsis. Similar functions are provided by suberin and its associated waxes that are localized in roots, seed coats, and abscission zones and are produced in response to wounding. This chapter focuses on the metabolic pathways that are associated with the biosynthesis and degradation of the acyl lipids mentioned above. These pathways, enzymes, and genes are also presented in detail in an associated website (ARALIP: http://aralip.plantbiology.msu.edu/). Protocols and methods used for analysis of Arabidopsis lipids are provided. Finally, a detailed summary of the composition of Arabidopsis lipids is provided in three figures and 15 tables. PMID:23505340

  9. Antioxidant activity of pomegranate peel extract on lipid and protein oxidation in beef meatballs during frozen storage.

    Science.gov (United States)

    Turgut, Sebahattin Serhat; Işıkçı, Fatma; Soyer, Ayla

    2017-07-01

    Antioxidant effect of pomegranate peel extract (PE) to retard lipid and protein oxidation in beef meatballs was investigated during frozen storage at -18±1°C. Concentrated and freeze dried aqueous extract of pomegranate peel was incorporated into freshly prepared meatball mix at 0.5% and 1.0% concentrations, and compared with 0.01% butylated hydroxytoluene (BHT) and control (without any antioxidant). In PE treated samples, particularly in high PE concentration, peroxide, malondialdehyde and carbonyl formation, loss of total protein solubility and sulfhydryl groups were significantly lower than control after 6months of storage. A diminution of both myofibrillar (MP) and sarcoplasmic (SP) proteins of high molecular weight was detected after 6months of the storage according to gel electrophoresis patterns. The 1.0% PE led to maintain colour intensity (C) and hue (h°) value. The results from sensory analyses revealed that PE addition to meatballs was effective on preventing rancid odour formation. Addition of both 0.5 and 1% PE in meatballs reduced lipid and protein oxidation and improved sensory scores. These results indicated that PE was effective on retarding lipid and protein oxidations. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus microcephalus

    Science.gov (United States)

    Wang, Xueying; Shi, Xuehui; Liu, Yifan; Yu, Daode; Guan, Shuguang; Liu, Qinghua; Li, Jun

    2016-07-01

    The present study evaluated the effects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (Gr), and lipid peroxidation (measured via malondialdehyde (MDA) content) were determined after the milt was stored at 4°C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol (PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation (only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could significantly improve the postthaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had significant effects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod.

  11. Storage of seeds of Cnidosculus phyllacanthus Pax & K. Hoffm. Armazenamento de sementes de Cnidosculus phyllacanthus Pax & K. Hoffm

    Directory of Open Access Journals (Sweden)

    Lígia M. de M. Silva

    2005-12-01

    Full Text Available This work aimed to determine the best environment for conservation of physiological quality of seeds of Cnidosculus phyllacanthus during storage. Seeds with 8.5% moisture content and 86% germinative capacity were filled in containers of different permeability, and storaged at different conditions during 360 days. Seeds packed in permeable container (paper bag were stored at ordinary room temperature (18 to 25 ºC and 55 to 78% RH, and dry chamber (18 ºC and 60% RH while those packed in semipermeable (polyethylene bag and impermeable (glass containers were stored in cold chamber (10 ºC and 75% RH. Seed moisture content, germinative capacity and germination speed were evaluated each 90 days interval. For all the tested storage conditions, seed germination speed was reduced at first evaluation and stabilized up to 360 days. Great deterioration in seeds stored at ordinary room condition was observed, while those stored in dry chamber maintained its germinative capacity for 270 days. The seed germinative capacity was better retained in cold storage, packaged either in semipemeable or impermeable containers. During storage, the seeds had a behavior classified as orthodox.Este trabalho foi desenvolvido com o objetivo de se verificar o melhor ambiente para conservação da qualidade fisiológica das sementes de Cnidosculus phyllacanthus (faveleira durante o armazenamento. Sementes com 8,5% de água e 86% de capacidade germinativa foram acondicionadas em embalagens de diferentes permeabilidades e armazenadas em ambientes diversos, por 360 dias. As sementes acondicionadas em embalagem permeável (saco de papel foram armazenadas nos ambientes natural de laboratório (18 a 25 ºC e 55 a 78% de umidade relativa-UR e de câmara seca (18 ºC e 60% UR, enquanto as acondicionadas em embalagens semipermeável (saco de polietileno e impermeável (recipiente de vidro foram armazenadas na câmara fria (10 ºC e 75% UR. O teor de água, a capacidade germinativa e a

  12. Diel pattern of circadian clock and storage protein gene expression in leaves and during seed filling in cowpea (Vigna unguiculata).

    Science.gov (United States)

    Weiss, Julia; Terry, Marta I; Martos-Fuentes, Marina; Letourneux, Lisa; Ruiz-Hernández, Victoria; Fernández, Juan A; Egea-Cortines, Marcos

    2018-02-14

    Cowpea (Vigna unguiculata) is an important source of protein supply for animal and human nutrition. The major storage globulins VICILIN and LEGUMIN (LEG) are synthesized from several genes including LEGA, LEGB, LEGJ and CVC (CONVICILIN). The current hypothesis is that the plant circadian core clock genes are conserved in a wide array of species and that primary metabolism is to a large extent controlled by the plant circadian clock. Our aim was to investigate a possible link between gene expression of storage proteins and the circadian clock. We identified cowpea orthologues of the core clock genes VunLHY, VunTOC1, VunGI and VunELF3, the protein storage genes VunLEG, VunLEGJ, and VunCVC as well as nine candidate reference genes used in RT-PCR. ELONGATION FACTOR 1-A (ELF1A) resulted the most suitable reference gene. The clock genes VunELF3, VunGI, VunTOC1 and VunLHY showed a rhythmic expression profile in leaves with a typical evening/night and morning/midday phased expression. The diel patterns were not completely robust and only VungGI and VungELF3 retained a rhythmic pattern under free running conditions of darkness. Under field conditions, rhythmicity and phasing apparently faded during early pod and seed development and was regained in ripening pods for VunTOC1 and VunLHY. Mature seeds showed a rhythmic expression of VunGI resembling leaf tissue under controlled growth chamber conditions. Comparing time windows during developmental stages we found that VunCVC and VunLEG were significantly down regulated during the night in mature pods as compared to intermediate ripe pods, while changes in seeds were non-significant due to high variance. The rhythmic expression under field conditions was lost under growth chamber conditions. The core clock gene network is conserved in cowpea leaves showing a robust diel expression pattern except VunELF3 under growth chamber conditions. There appears to be a clock transcriptional reprogramming in pods and seeds compared to

  13. The Effect of Different Dietary Purslane Seed (Portulaca Oleracea L. Levels on Carcass, Blood Lipid Profile and Antioxidant Activity in Quails

    Directory of Open Access Journals (Sweden)

    Yusuf Konca

    2016-01-01

    Full Text Available This study was carried out to determine the effect of dietary purslane seed (Portulaca oleracea L. diets on the carcass, serum lipid profile and antioxidant activity in quails. A total of 140 one-day-old quail chicks allocated into 4 treatment groups with 5 replicates. The treatment groups as follows: (1 Control (C, without purslane seed addition, (2 2.5% purslane seed addition to the control diet, (3 5% purslane seed addition to the control diet, (4 10% purslane seed addition to the control diet. The treatments did not affect the slaughter weight, carcass, and carcass part yields (p>0.05 of the birds in the treatment groups. However, the total intestinal weights of the birds in the 2.5% purslane supplemented group were lower than those of the other groups (p0.05. The dietary inclusion of 10% purslane seed caused an increase to MDA level compared to C and 2.5% purslane seed supplemented groups (p

  14. Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice

    NARCIS (Netherlands)

    Chen, Y.; Sun, A.; Wang, M.; Zhu, Z.; Ouwerkerk, P.B.F.

    2014-01-01

    Glutelins are the most abundant storage proteins in rice grain and can make up to 80 % of total protein content. The promoter region of GluB-1, one of the glutelin genes in rice, has been intensively used as a model to understand regulation of seed-storage protein accumulation. In this study, we

  15. Lipid oxidative changes in traditional dry fermented sausage Petrovská klobása during storage

    Directory of Open Access Journals (Sweden)

    Šojić Branislav V.

    2014-01-01

    Full Text Available The influence of drying and ripeninig conditions (traditional and industrial in the production of dry fermented sausage Petrovská klobása, on fatty-acid composition and oxidative changes in lipids, during 7 months of storage, was investigated. During the storage period, the sum of unsaturated fatty acids and the content of free fatty acids were significantly higher (p<0.05, while the content of malondialdehyde was significantly lower in the sausage subjected to traditional conditions of drying and ripening. At the end of the storage period, contents of pentanal and hexanal in the sausage subjected to traditional conditions of drying and ripening (4.03 μg/g and 1.67 μg/g, respectively were significantly lower (p<0.05 in comparison with these contents in the sausage subjected to industrial conditions of drying and ripening. Traditional conditions of drying and ripening at lower temperatures have led to lower oxidative changes in lipids in traditional dry fermented sausage Petrovská klobása during storage period. [Projekat Ministarstva nauke Republike Srbije, br. TR31032

  16. Assessment of the Antioxidant Activity of Silybum marianum Seed Extract and Its Protective Effect against DNA Oxidation, Protein Damage and Lipid Peroxidation

    Directory of Open Access Journals (Sweden)

    Aynur Serçe

    2016-01-01

    Full Text Available Antioxidant properties of ethanol extract of Silybum marianum (milk thistle seeds was investigated. We have also investigated the protein damage activated by oxidative Fenton reaction and its prevention by Silybum marianum seed extract. Antioxidant potential of Silybum marianum seed ethanol extract was measured using diff erent in vitro methods, such as lipid peroxidation, 1,1–diphenyl–2–picrylhydrazyl (DPPH and ferric reducing power assays. The extract significantly decreased DNA damage caused by hydroxyl radicals. Protein damage induced by hydroxyl radicals was also effi ciently inhibited, which was confirmed by the presence of protein damage markers, such as protein carbonyl formation and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE. The present study shows that milk thistle seeds have good DPPH free radical scavenging activity and can prevent lipid peroxidation. Therefore, Silybum marianum can be used as potentially rich source of antioxidants and food preservatives. The results suggest that the seeds may have potential beneficial health effects providing opportunities to develop value-added products.

  17. Influence of cooking methods and storage time on lipid and protein oxidation and heterocyclic aromatic amines production in bacon.

    Science.gov (United States)

    Soladoye, O P; Shand, P; Dugan, M E R; Gariépy, C; Aalhus, J L; Estévez, M; Juárez, M

    2017-09-01

    This study aimed to examine the influence of cooking methods and pre-determined refrigerated storage days on the production of lipid oxidation (TBARS), protein oxidation (PROTOX) and heterocyclic aromatic amines (HAA) in bacon. Forty-four pork bellies selected from pigs varying in breed, sex and diets to introduce variability in composition were processed as bacon. Sliced-bacon was stored at 4°C either for 2 or 28days and these storage groups were cooked either with microwave or frying pan. Microwave led to significantly higher PROTOX (P0.05) by the cooking methods and storage times. Similarly, the fatty acid composition of pork belly did not significantly influence the production of HAA, TBARS and PROTOX produced in bacon during cooking. Overall, microwave cooking had lesser impact on the production of carcinogenic compounds in bacon with only minor impact on sensory attributes. Copyright © 2017. Published by Elsevier Ltd.

  18. Changes in soluble carbohydrates during storage of Caesalpinia echinata LAM. (Brazilwood seeds, an endangered leguminous tree from the Brazilian Atlantic Forest

    Directory of Open Access Journals (Sweden)

    I. S. Garcia

    Full Text Available Caesalpinia echinata seeds stored in laboratory environmental conditions lose their viability in one month whilst under low temperatures germination is maintained for 18 months of storage. These seeds are tolerant to desiccation, keeping their viability up to 0.08 gH2O.gDW-1. Since soluble carbohydrates are believed to be involved with desiccation tolerance and seed storability, the aim of this work is to analyze the content and composition of soluble carbohydrates in C. echinata seeds during storage in paper bags (PB and glass flasks (GF at laboratory room (RT and cool (CT temperatures. In freshly harvested seeds, total soluble carbohydrates comprised approximately 10% of the dry weight, decreasing to ca. 8% over 18 months of storage at RT. In seeds stored at CT, sugars varied differently decreasing initially and being restored at the end of the analysis period. The main neutral sugars in seeds from all treatments were sucrose, fructose and glucose. Raffinose and stachyose were present as traces. Free myo-inositol and other cyclitols were also detected. The main tendency observed was the variation in levels of both glucose and fructose in relation to sucrose, the highest levels of monosaccharides which were found in seeds stored at CT. The values of glucose and fructose were practically constant in seeds stored in paper bags for 18 months at CT, decreasing consistently in the other treatments, mainly at RT. Sucrose contents remained relatively stable. Changes in soluble sugars during storage suggest that the loss of germinability of seeds of C. echinata could be associated with low levels of glucose and fructose in relation to sucrose.

  19. Marine Lipids (Omega-3 Oil) - Stability of Oil and Enriched Products During Production and Storage

    DEFF Research Database (Denmark)

    Nielsen, Nina Skall

    2015-01-01

    The awareness of health benefits of marine lipids with a high content of omega-3 poly unsaturated fatty acids from fish and algae oil has led to an increased intake as oil and in products. However, these lipids are highly susceptible to lipid oxidation, which results in the formation of undesirable...... off-flavours and gives rise to unhealthy compounds such as free radicals and reactive aldehydes. Necessary prerequisites for successful development of omega-3 enriched products are that the oil used for enrichment is of a high quality and low in oxidation products and that oxidation of the lipids...

  20. Distinct populations of hepatic stellate cells in the mouse liver have different capacities for retinoid and lipid storage.

    Directory of Open Access Journals (Sweden)

    Diana N D'Ambrosio

    Full Text Available Hepatic stellate cell (HSC lipid droplets are specialized organelles for the storage of retinoid, accounting for 50-60% of all retinoid present in the body. When HSCs activate, retinyl ester levels progressively decrease and the lipid droplets are lost. The objective of this study was to determine if the HSC population in a healthy, uninjured liver demonstrates heterogeneity in its capacity for retinoid and lipid storage in lipid droplets. To this end, we utilized two methods of HSC isolation, which leverage distinct properties of these cells, including their vitamin A content and collagen expression. HSCs were isolated either from wild type (WT mice in the C57BL/6 genetic background by flotation in a Nycodenz density gradient, followed by fluorescence activated cell sorting (FACS based on vitamin A autofluorescence, or from collagen-green fluorescent protein (GFP mice by FACS based on GFP expression from a GFP transgene driven by the collagen I promoter. We show that GFP-HSCs have: (i increased expression of typical markers of HSC activation; (ii decreased retinyl ester levels, accompanied by reduced expression of the enzyme needed for hepatic retinyl ester synthesis (LRAT; (iii decreased triglyceride levels; (iv increased expression of genes associated with lipid catabolism; and (v an increase in expression of the retinoid-catabolizing cytochrome, CYP2S1.Our observations suggest that the HSC population in a healthy, uninjured liver is heterogeneous. One subset of the total HSC population, which expresses early markers of HSC activation, may be "primed" and ready for rapid response to acute liver injury.

  1. Pengaruh pemberian abu sekam padi sebagai bahan desikan pada penyimpanan benih terhadap daya tumbuh dan pertumbuhan bibit kakao (The effects of rice husk ash as desiccation material of seed storage on viability and cocoa seedling growth.

    Directory of Open Access Journals (Sweden)

    Pudji Rahardjo

    2012-08-01

    Full Text Available Rice husk ash as desiccation material can be used to maintain seed viability in storage through its ability to absorb humidity during its storage. High relative humidity caused seed moisture content to increase so the respiration rate of seed increases and uses faster food stock. Finally the viability of seed is lost. A research on use of rice husk ash as desiccation material of cocoa seed storage was conducted in Agronomy Laboratory and Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institute. Completely Randomized Design was used in this research with treatment of rice husk ash application on cocoa seed as follows: 0 g/100 seeds (A, 5 g/100 seeds (B, 10 g/100 seeds (C, 15 g/100 seeds (D, and 20 g/100 seeds (E. This experiment used four replications. Cocoa seeds were stored in plastic bag within carton box in ambient temperature. The storage periods were 1, 2, and 3 weeks, and parameters of observation consisted of electrical conductivity of dipped water of cocoa seeds, percentage of seed germination, percentage of seed emergence, early growth parameters at one month old including seedlings height of seedling, diameter, leaf number, root length, and dry weight. The result of the experiment showed that the use of rice husk ash at 5-10 g/100 seeds could maintain electrical conductivity of dipped water at low level, percentage of seed germination at 99-100 % and percentage of seed emergence at 79-91% after two weeks storage. The use of rice husk ash at 5-10 g/100 seeds after two weeks storage affected height of cacao seedling, but did not affected stem diameters, leaf numbers, root lengths, and dry weights

  2. Effect Of Light Qualities And Storage Periods On The Germination Of Pennisetum Polystachion Seeds*)

    OpenAIRE

    TJITROSEMITO, S

    1992-01-01

    Seeds of the yellowish inflorescence strain of Pennisetum polystachion, collected from the field in Indonesia, were kept in the dark for 30 days, then germinated in 12-h light and 24-h light under various light qualities, namely, white, black, blue, red and far-red. There was no effect of photo-period to seed germination. Percent of seed germination under white, red, far-red, blue and dark were 49, 43, 22, 11 and 2%, respectively. White and red light did not cause any diffe...

  3. Storage behavior and changes in concentrations of abscisic acid and gibberellins during dormancy break and germination in seeds of Phellodendron amurense var. wilsonii (Rutaceae).

    Science.gov (United States)

    Chen, Shun-Ying; Chien, Ching-Te; Baskin, Jerry M; Baskin, Carol C

    2010-02-01

    The medicinal Asian plant genus Phellodendron is known to contain several very important compounds that have biological action. The main purpose of this study was to determine whether seeds of Phellodendron amurense var. wilsonii can be stored and to characterize their dormancy. Seeds of this taxon stored at -20 and -80 degrees C and in liquid nitrogen retained their high germinability, indicating that they have orthodox storage behavior. Intact seeds from freshly collected fruits were dormant and required 12 weeks of cold stratification at 4 degrees C for complete germination. Scarifying the seed coat was partially effective in breaking seed dormancy. Exogenous gibberellins (GA(3), GA(4) and GA(4+7)) promoted germination of scarified seeds, GA(4) and GA(4+7) being more effective than GA(3). Fluridone, an abscisic acid (ABA) biosynthesis inhibitor, was efficient in breaking dormancy, but it was less effective than GA(4) or GA(4+7) alone. Paclobutrazol, a GA biosynthesis inhibitor, inhibited seed germination, and the inhibitory effect was reversed completely by GA(4) and by GA(4+7). ABA content of seeds subjected to cold stratification or to incubation at 35/10 degrees C, which enhanced seed germination, was reduced about four- to sixfold compared to that of fresh seeds. Higher concentrations of GA(3), GA(4) and GA(7) were detected in nondormant seeds and in seeds with an emerged radicle than in fresh seeds. Present results seem to indicate that dormancy in P. amurense var. wilsonii seeds is imposed partially by the seed coat and partially by high ABA content. ABA content decreased and GA(3), GA(4) and GA(7) content increased during germination.

  4. Mechanisms Involved in the Improvement of Lipotoxicity and Impaired Lipid Metabolism by Dietary α-Linolenic Acid Rich Salvia hispanica L (Salba) Seed in the Heart of Dyslipemic Insulin-Resistant Rats

    Science.gov (United States)

    Creus, Agustina; Ferreira, María R.; Oliva, María E.; Lombardo, Yolanda B.

    2016-01-01

    This study explores the mechanisms underlying the altered lipid metabolism in the heart of dyslipemic insulin-resistant (IR) rats fed a sucrose-rich diet (SRD) and investigates if chia seeds (rich in α-linolenic acid 18:3, n-3 ALA) improve/reverse cardiac lipotoxicity. Wistar rats received an SRD-diet for three months. Half of the animals continued with the SRD up to month 6. The other half was fed an SRD in which the fat source, corn oil (CO), was replaced by chia seeds from month 3 to 6 (SRD+chia). A reference group consumed a control diet (CD) all the time. Triglyceride, long-chain acyl CoA (LC ACoA) and diacylglycerol (DAG) contents, pyruvate dehydrogenase complex (PDHc) and muscle-type carnitine palmitoyltransferase 1 (M-CPT1) activities and protein mass levels of M-CPT1, membrane fatty acid transporter (FAT/CD36), peroxisome proliferator activated receptor α (PPARα) and uncoupling protein 2 (UCP2) were analyzed. Results show that: (a) the hearts of SRD-fed rats display lipotoxicity suggesting impaired myocardial lipid utilization; (b) Compared with the SRD group, dietary chia normalizes blood pressure; reverses/improves heart lipotoxicity, glucose oxidation, the increased protein mass level of FAT/CD36, and the impaired insulin stimulated FAT/CD36 translocation to the plasma membrane. The enhanced M-CPT1 activity is markedly reduced without similar changes in protein mass. PPARα slightly decreases, while the UCP2 protein level remains unchanged in all groups. Normalization of dyslipidemia and IR by chia reduces plasma fatty acids (FAs) availability, suggesting that a different milieu prevents the robust translocation of FAT/CD36. This could reduce the influx of FAs, decreasing the elevated M-CPT1 activity and lipid storage and improving glucose oxidation in cardiac muscles of SRD-fed rats. PMID:26828527

  5. Protective effects of Opuntia ficus-indica extract on ram sperm quality, lipid peroxidation and DNA fragmentation during liquid storage.

    Science.gov (United States)

    Allai, Larbi; Druart, Xavier; Öztürk, Mehmet; BenMoula, Anass; Nasser, Boubker; El Amiri, Bouchra

    2016-12-01

    The present study aimed to assess the phenolic composition of the acetone extract from Opuntia ficus indica cladodes (ACTEX) and its effects on ram semen variables, lipid peroxidation and DNA fragmentation during liquid storage at 5°C for up to 72h in skim milk and Tris egg yolk extenders. Semen samples from five rams were pooled extended with Tris-egg yolk (TEY) or skim milk (SM) extenders containing ACTEX (0%, 1%, 2%, 4% and 8%) at a final concentration of 0.8×10 9 sperm/ml and stored for up to 72h at 5°C. The sperm variables were evaluated at different time periods (8, 24, 48 and 72h). Sperm total motility and viability were superior in TEY than in SM whereas the progressive motility, membrane integrity, abnormality and spontaneous lipid peroxidation were greater in SM compared to TEY (P<0.05). The results also indicated that the inclusion of 1% ACTEX in the SM or TEY extender increased the sperm motility, viability, membrane integrity, and decreased the abnormality, lipids peroxidation up to 72h in storage compared to control group. Similarly, even at 72h of storage, 1% ACTEX can efficiently decrease the negative effects of liquid storage on sperm DNA fragmentation (P<0.05). In conclusion, SM and TEY supplemented with 1% of ACTEX can improve the quality of ram semen. Further studies are required to identify the active components in ACTEX involved in its effect on ram sperm preservation. Copyright © 2016 Elsevier B.V. All rights reserved.

  6. Preservation of seed viability during 25 years of storage under standard genebank conditions

    NARCIS (Netherlands)

    Treuren, van R.; Groot, de E.C.; Hintum, van T.J.L.

    2013-01-01

    Maintaining sufficient viability is critical to the sustainability of ex situ conserved seed collections. For this reason, accessions are regenerated when viability falls below a predefined threshold. Viability is monitored by determining the germination ability of accessions at predefined time

  7. Isolation and structure elucidation of avocado seed (Persea americana) lipid derivatives that inhibit Clostridium sporogenes endospore germination.

    Science.gov (United States)

    Rodríguez-Sánchez, Dariana Graciela; Pacheco, Adriana; García-Cruz, María Isabel; Gutiérrez-Uribe, Janet Alejandra; Benavides-Lozano, Jorge Alejandro; Hernández-Brenes, Carmen

    2013-07-31

    Avocado fruit extracts are known to exhibit antimicrobial properties. However, the effects on bacterial endospores and the identity of antimicrobial compounds have not been fully elucidated. In this study, avocado seed extracts were tested against Clostridium sporogenes vegetative cells and active endospores. Bioassay-guided purification of a crude extract based on inhibitory properties linked antimicrobial action to six lipid derivatives from the family of acetogenin compounds. Two new structures and four compounds known to exist in nature were identified as responsible for the activity. Structurally, most potent molecules shared features of an acetyl moiety and a trans-enone group. All extracts produced inhibition zones on vegetative cells and active endospores. Minimum inhibitory concentrations (MIC) of isolated molecules ranged from 7.8 to 15.6 μg/mL, and bactericidal effects were observed for an enriched fraction at 19.5 μg/mL. Identified molecules showed potential as natural alternatives to additives and antibiotics used by the food and pharmaceutical industries to inhibit Gram-positive spore-forming bacteria.

  8. Pulmonary functions and sleep-related breathing disorders in lipid storage disease.

    Science.gov (United States)

    Bingöl, Züleyha; Tekce, Hacer Durmuş; Sağcan, Gülseren; Serdaroğlu, Piraye; Kıyan, Esen

    2018-03-01

    Pulmonary function abnormalities and sleep-related breathing disorders (SRBD) are frequent in subjects with several neuromuscular diseases but there is no data about lipid storage diseases (LSD). Therefore, we aimed to evaluate pulmonary functions and SRBD in adults with LSD. Pulmonary functions (forced expiratory volume (FEV 1 ), forced vital capacity (FVC), supine FVC, upright-supine FVC% change, maximal inspiratory pressure (MIP), maximal expiratory pressure (MEP), peak cough flow (PCF)), arterial blood gases, and polysomnographic data of all subjects were evaluated. Twenty-five subjects with LSD were evaluated [17 males, 8 females; age 34.9 ± 15 years; BMI 26.5 ± 3.4 kg/m 2 ]. MIP was - 72.2 ± 32.7 cmH 2 O ( 45 mmHg). REM sleep had decreased in all subjects (10.2% ± 6.1). Obstructive sleep apnea (OSA) was found in 80% of the subjects (n = 20; 9 mild, 9 moderate, 2 severe). For subjects with OSA, apnea-hypopnea index (AHI) was 20.8 ± 15.9/h, oxygen desaturation index (ODI) was 11.9 ± 15.4/h, AHI REM was 30.6 ± 19.7/h, AHI NREM was 19.7 ± 16.6/h, ODI REM was 27.2 ± 26.1/h, and ODI NREM was 11.4 ± 15/h. Five subjects (20%) diagnosed as REM-related OSA. Nocturnal mean SpO 2 was 94.9% ± 1.7, lowest SpO 2 was 73.3% ± 13.9, and time spent with SpO 2 < 90% was 2.4% ± 7.2. In subjects with LSD, pulmonary function impairment, daytime hypercapnia and hypoxemia, and OSA, especially REM-related OSA, are frequent. Therefore, pulmonary functions and polysomnography should be performed routinely.

  9. The Effect of Fenugreek (Trigonella foenum-graecum Seed and 17-β Estradiol on Serum Apelin, Glucose, Lipids, and Insulin in Ovariectomized Rats

    Directory of Open Access Journals (Sweden)

    Abedinzade

    2015-08-01

    Full Text Available Background Menopause, a natural phenomenon, is defined by the fall of ovarian hormones mainly estrogens causing major problems such as insulin resistance. Fenugreek (Trigonella foenum-graecum is known to have some useful properties such as insulin sensitizing effect. Apelin is an adipokine, which has several roles such as regulation of insulin secretion. Objectives The objective of the present study was to evaluate the effect of fenugreek seed and 17-β estradiol on serum Apelin along with glucose, lipids and insulin in ovariectomized rats. Materials and Methods Forty-nine adult female Wistar rats were randomly divided to seven groups: normal control, ovariectomized control, ovariectomized treated with ethanolic and hexanic extract of fenugreek seed (50 and 150 mg/kg/daily for each, and ovariectomized treated with 17-β estradiol (10 µg/kg/daily for 42 days. Serum Apelin, glucose, lipids and insulin were measured. Results Serum Apelin, glucose, lipids and insulin significantly increased in ovariectomized controls in comparison with normal controls (P < 0.05. Serum glucose, lipids and insulin in ovariectomized rats treated with fenugreek seed extract and 17-β estradiol were remarkably lower than ovariectomized controls (P < 0.05. Furthermore, 17-β estradiol caused a significant decrease (P < 0.05 in serum Apelin in ovariectomized rats. Conclusions It appears that fenugreek seed might be effective against hyperglycemia, hyperlipidemia and insulin resistance in ovariectomized rats without impact on serum Apelin. Furthermore, 17-β estradiol could have similar effects along with possible inhibitory effects on serum Apelin. The complicated role of Apelin in menopause needs to be further explored.

  10. Antioxidant effects of soy sauce on color stability and lipid oxidation of raw beef patties during cold storage.

    Science.gov (United States)

    Kim, Hyun-Wook; Choi, Yun-Sang; Choi, Ji-Hun; Kim, Hack-Youn; Hwang, Ko-Eun; Song, Dong-Heon; Lee, Soo-Yoen; Lee, Mi-Ai; Kim, Cheon-Jei

    2013-11-01

    This study was conducted to evaluate the antioxidant effects of soy sauce on lipid oxidation and color stability of raw beef patties. Raw beef patties were formulated with four solutions such as NaCl (sodium chloride solution), NaCl/SS (1:1 ratio of sodium chloride and soy sauce solution), SS (soy sauce solution), or SS/A (soy sauce solution combined with 0.05% ascorbic acid) in the same salt concentration. Addition of soy sauce resulted in the decreased pH, lightness, and increased yellowness. Treatment SS/A had the lowest percent of metmyoglobin during storage (Psauce addition were observed in treatments SS and SS/A at the end of the storage period. There were no differences (P>0.05) in free fatty acid concentration at the end of storage. The combined addition of soy sauce and ascorbic acid greatly improved (P<0.05) color stability and retarded lipid oxidation. Copyright © 2013 Elsevier Ltd. All rights reserved.

  11. Tratamento inseticida e qualidade de sementes de milho durante o armazenamento Insecticide treatment and seed quality of corn during storage

    Directory of Open Access Journals (Sweden)

    Oscar José Smiderle

    1999-01-01

    test, cold test, accelerated aging, electric conductivity test, emergence of seedlings in field and rate of seedling emergence, seven times in bimonthly intervals. Interpretation of the results showed that: deltamethrin and chlorpyrifos insecticides (alone or combined, phosphin and diatomaceous earth promoted the control in a similar manner, of pest-insects occuring during storage without causing toxicity to corn seeds. The physiological quality of corn seeds was effectively preserved by the addition of chemical insecticides and diatomaceous earth.

  12. Unsaturated Lipids Change in Olive Tree Drupe and Seed during Fruit Development and in Response to Cold-Stress and Acclimation

    Directory of Open Access Journals (Sweden)

    Simone D’Angeli

    2016-11-01

    Full Text Available The olive tree is a plant of economic value for the oil of its drupe. It is a cultigen complex composed of genotypes with differences in cold-hardiness. About 90% of the oil is stored in oil bodies (OBs in the drupe during the oleogenic phase. Phenols and lipids contribute to oil quality, but the unsaturated fatty acid (FA fraction is emerging as the most important for quality, because of the very high content in oleic acid, the presence of ω6-linoleic acid and ω3-linolenic acid, and the very low saturated FA content. Another 10% of oil is produced by the seed. Differences in unsaturated FA-enriched lipids exist among seed coat, endosperm, and embryo. Olive oil quality is also affected by the environmental conditions during fruit growth and genotype peculiarities. Production of linoleic and α-linolenic acids, fruit growth, fruit and leaf responses to low temperatures, including cuticle formation, and cold-acclimation are related processes. The levels of unsaturated FAs are changed by FA-desaturase (FAD activities, involving the functioning of chloroplasts and endoplasmic reticulum. Cold induces lipid changes during drupe and seed development, affecting FADs, but its effect is related to the genotype capability to acclimate to the cold.

  13. Assessment of genetic diversity among some accessions of sage (Salvia officinalis L. using electrophoresis of seed storage proteins

    Directory of Open Access Journals (Sweden)

    Seyed Abbas Mirjalili

    2016-06-01

    Full Text Available The genus Salvia (Lamiaceae comprises over 900 species in the world, with a relatively wide dispersion in the Iran’s flora. Until now, about 58 species of the genus have been reported and identified in Iran, in which 17 of them were endemic. In order to study, investigate and evaluate the intraspecific diversity, similarity and dissimilarity among Iranian Salvia officinalis accessions, an experiment was carried out using SDS-PAGE technique. In this study, the seeds from five accessions were collected from gene bank and were evaluated. The seed storage proteins were extracted by buffers and were measured. Phylogenetic relationships were analyzed according to presence and absence of bands on the gel. A dendrogram was prepared using calculation of the accession’s similarity index. Mean comparison were done by Tukey’s test. The seed protein contents showed significant differences (p≤0.01 among accessions. A total of 39 bands were indicated on the gel. The maximum diversity was detected in the accession No. 2 while, the lowest band’s number were recorded with the accessions No. 1 and 5. Based on dendrogram, the accessions were divided into two groups; one includes accession No. 1 and 4 other accessions were located in the second group further classified into two subgroup including accessions No. 2 and 3 in one clade and accessions No. 4 and 5 in the other ones.

  14. N-terminomics reveals control of Arabidopsis seed storage proteins and proteases by the Arg/N-end rule pathway.

    Science.gov (United States)

    Zhang, Hongtao; Gannon, Lucy; Hassall, Kirsty L; Deery, Michael J; Gibbs, Daniel J; Holdsworth, Michael J; van der Hoorn, Renier A L; Lilley, Kathryn S; Theodoulou, Frederica L

    2018-05-01

    The N-end rule pathway of targeted protein degradation is an important regulator of diverse processes in plants but detailed knowledge regarding its influence on the proteome is lacking. To investigate the impact of the Arg/N-end rule pathway on the proteome of etiolated seedlings, we used terminal amine isotopic labelling of substrates with tandem mass tags (TMT-TAILS) for relative quantification of N-terminal peptides in prt6, an Arabidopsis thaliana N-end rule mutant lacking the E3 ligase PROTEOLYSIS6 (PRT6). TMT-TAILS identified over 4000 unique N-terminal peptides representing c. 2000 protein groups. Forty-five protein groups exhibited significantly increased N-terminal peptide abundance in prt6 seedlings, including cruciferins, major seed storage proteins, which were regulated by Group VII Ethylene Response Factor (ERFVII) transcription factors, known substrates of PRT6. Mobilisation of endosperm α-cruciferin was delayed in prt6 seedlings. N-termini of several proteases were downregulated in prt6, including RD21A. RD21A transcript, protein and activity levels were downregulated in a largely ERFVII-dependent manner. By contrast, cathepsin B3 protein and activity were upregulated by ERFVIIs independent of transcript. We propose that the PRT6 branch of the pathway regulates protease activities in a complex manner and optimises storage reserve mobilisation in the transition from seed to seedling via control of ERFVII action. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

  15. Investigation of total seed storage proteins of pakistani and japanese maize (zea mays l.) through sds-page markers

    International Nuclear Information System (INIS)

    Shinwari, Z.K.

    2014-01-01

    The assessment of genetic diversity among the members of a species is of vital importance for successful breeding and adaptability. In the present study 83 genotypes of maize of Pakistani and Japanese origin were evaluated for the total seed storage proteins using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) through vertical slab unit. The total protein subunits were separated on 12% polyacrylamide gel using standard protocols. A total of 18 protein subunits were noted out of which 7 (39%) were monomorphic and 11 (61%) were polymorphic, with molecular weight ranging from 10 to 122 kDa. Coefficients of similarity among the accessions ranged between 0.89 and 1.00. The dendrogram obtained through UPGMA clustering method showed two main clusters: 1 and 2. First cluster comprised of 9 genotypes including Sahiwal-2002, while second cluster contained 74 genotypes including Aaiti-2002 and Sadaf. Over all a low level of polymorphism was observed in total seed storage protein patterns of maize genotypes from Pakistan as well as Japan. It is inferred from the present study that more genotypes of maize could be brought under study and more advanced biochemical techniques with more reliable results could be followed to bring assessment of genetic diversity of maize for planning breeding programs. (author)

  16. Severe steatohepatitis in a patient with a rare neutral lipid storage disorder due to ABHD5 mutation.

    Science.gov (United States)

    Ronchetti, Anna; Prati, Daniele; Pezzotta, Maria Grazia; Tavian, Daniela; Colombo, Roberto; Callea, Francesco; Colli, Agostino

    2008-09-01

    Fatty liver disease is mainly caused by alcohol consumption, excessive body weight, dyslipidemia and impaired glucose tolerance, but inherited disorders can sometimes be involved. We report the case of a 40-year-old woman with steatohepatitis and severe portal hypertension, associated with ichthyosis, cataract and hypoacusia. The clinical, pathological and genetic findings were consistent with a diagnosis of Chanarin-Dorfman syndrome (CDS), a rare autosomal recessive inherited neutral lipid storage disorder, and genetic analysis showed that a novel ABHD5 mutation is responsible.

  17. Terminalia arjuna: A novel natural preservative for improved lipid oxidative stability and storage quality of muscle foods

    OpenAIRE

    Insha Kousar Kalem; Z.F. Bhat; Sunil Kumar; Ajay Desai

    2017-01-01

    The study was conducted to explore the possibility of utilization of Terminalia arjuna as a novel natural preservative in meat products by using chevon sausages as a model system. Chevon sausages were prepared by incorporating different levels of T. arjuna viz. T1 (0.25%), T2 (0.50%) and T3 (0.75%) and were assessed for various lipid oxidative stability and storage quality parameters under refrigerated (4 ± 1 °C) conditions. T. arjuna showed a significant (p 

  18. Regulation of fatty acid composition and lipid storage by thyroid hormone in mouse liver

    OpenAIRE

    Yao, Xuan; Hou, Sarina; Zhang, Duo; Xia, Hongfeng; Wang, Yu-Cheng; Jiang, Jingjing; Yin, Huiyong; Ying, Hao

    2014-01-01

    Background Thyroid hormones (THs) are potent hormones modulating liver lipid homeostasis. The perturbation of lipid homeostasis is a hallmark of non-alcoholic fatty liver disease (NAFLD), a very common liver disorder. It was reported that NAFLD patients were associated with higher incidence of hypothyroidism. However, whether abnormal thyroid function contributes to the pathogenesis of NAFLD remains unclear. Results We used in vivo models to investigate the influence of hypothyroidism and TH ...

  19. Anti obese potential of Cucurbita maxima seeds oil: effect on lipid profile and histoarchitecture in high fat diet induced obese rats.

    Science.gov (United States)

    Kalaivani, A; Sathibabu Uddandrao, V V; Brahmanaidu, P; Saravanan, Ganapathy; Nivedha, P R; Tamilmani, P; Swapna, K; Vadivukkarasi, Sasikumar

    2017-10-19

    In this study, we made an attempt to evaluate the potential of Cucurbita maxima seeds oil (CSO) against high-fat diet (HFD)-induced obesity in rats. We investigated the effect of CSO (100 mg/kg body weight) supplementation over 30 days on the changes of HFD-induced obese rats in body weight, biochemical parameters and lipid profile as well as investigated the effects of CSO on the histopathological changes. Oral administration with CSO revealed significant diminution in body weight gain, glucose and insulin levels, which altered the activity of lipid profile and restored the pathological alterations. It demonstrated that CSO had considerably altered these parameters when evaluated with HFD control rats. In conclusion, this study established that CSO prevents the HFD-induced obesity by altering the markers important to lipid metabolism.

  20. Fat storage-inducing transmembrane (FIT or FITM proteins are related to lipid phosphatase/phosphotransferase enzymes

    Directory of Open Access Journals (Sweden)

    Matthew J Hayes

    2017-12-01

    Full Text Available Fat storage-inducing transmembrane (FIT or FITM proteins have been implicated in the partitioning of triacylglycerol to lipid droplets and the budding of lipid droplets from the ER. At the molecular level, the sole relevant interaction is that FITMs directly bind to triacyglycerol and diacylglycerol, but how they function at the molecular level is not known. Saccharomyces cerevisiae has two FITM homologues: Scs3p and Yft2p. Scs3p was initially identified because deletion leads to inositol auxotrophy, with an unusual sensitivity to addition of choline. This strongly suggests a role for Scs3p in phospholipid biosynthesis. Looking at the FITM family as widely as possible, we found that FITMs are widespread throughout eukaryotes, indicating presence in the last eukaryotic common ancestor. Protein alignments also showed that FITM sequences contain the active site of lipid phosphatase/phosphotransferase (LPT enzymes. This large family transfers phosphate-containing headgroups either between lipids or in exchange for water. We confirmed the prediction that FITMs are related to LPTs by showing that single amino-acid substitutions in the presumptive catalytic site prevented their ability to rescue growth of the mutants on low inositol/high choline media when over-expressed. The substitutions also prevented rescue of other phenotypes associated with loss of FITM in yeast, including mistargeting of Opi1p, defective ER morphology, and aberrant lipid droplet budding. These results suggest that Scs3p, Yft2p and FITMs in general are LPT enzymes involved in an as yet unknown critical step in phospholipid metabolism.

  1. Investigation of Lipid Metabolism by a New Structured Lipid with Medium- and Long-Chain Triacylglycerols from Cinnamomum camphora Seed Oil in Healthy C57BL/6J Mice.

    Science.gov (United States)

    Hu, Jiang-Ning; Shen, Jin-Rong; Xiong, Chao-Yue; Zhu, Xue-Mei; Deng, Ze-Yuan

    2018-02-28

    In the present study, a new structured lipid with medium- and long-chain triacylglycerols (MLCTs) was synthesized from camellia oil (CO) and Cinnamomum camphora seed oil (CCSO) by enzymatic interesterification. Meanwhile, the antiobesity effects of structured lipid were investigated through observing the changes of enzymes related to lipid mobilization in healthy C57BL/6J mice. Results showed that after synthesis, the major triacylgeride (TAG) species of intesterificated product changed to LaCC/CLaC (12.6 ± 0.46%), LaCO/LCL (21.7 ± 0.76%), CCO/LaCL (14.2 ± 0.55%), COO/OCO (10.8 ± 0.43%), and OOO (18.6 ± 0.64%). Through second-stage molecular distillation, the purity of interesterified product (MLCT) achieved 95.6%. Later, male C57BL/6J mice were applied to study whether the new structured lipid with MLCT has the efficacy of preventing the formation of obesity or not. After feeding with different diets for 6 weeks, MLCTs could reduce body weight and fat deposition in adipose tissue, lower plasma triacylglycerols (TG) (0.89 ± 0.16 mmol/L), plasma total cholesterol (TC) (4.03 ± 0.08 mmol/L), and hepatic lipids (382 ± 34.2 mg/mice) by 28.8%, 16.0%, and 30.5%, respectively, when compared to the control 2 group. This was also accompanied by increasing fecal lipids (113%) and the level of enzymes including cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), hormone-sensitive lipase (HSL), and adipose triglyceride lipase (ATGL) related to lipid mobilization in MLCT group. From the results, it can be concluded that MLCT reduced body fat deposition probably by modulating enzymes related to lipid mobilization in C57BL/6J mice.

  2. Survival and vigour of ultra-dry seeds after ten years of hermetic storage

    NARCIS (Netherlands)

    Hong, T.D.; Ellis, R.H.; Astley, D.; Pinnegar, A.E.; Groot, S.P.C.; Kraak, H.L.

    2005-01-01

    Seeds of carrot, groundnut, lettuce, oilseed rape and onion were stored hermetically in laminated aluminium foil packets in four environments (dry or ultra-dry moisture contents combined factorially with temperatures of 20 degrees C or -20 degrees C), replicated at several sites. After ten years'

  3. Effect of irradiation and soaking in BHT and sodium pyrophosphate on meat proteins and lipids during cold storage

    International Nuclear Information System (INIS)

    Hassan, I.M.; Emam, O.A.

    1988-01-01

    The effect of irradiation treatments up to 10 KGy, soaking in a solution containing 0.5% Na-pyrophosphate and 250 ppm butylated hydroxy toluene (BHT) and a combination of both treatments on the nitrogen content and solubility, protein fractions and lipids stability in beef steaks during cold storage at 4 ± 1°C was followed until the samples were rejected by sensory evaluation. The least effective radiation doses for soluble protein nitrogen (SPN), total soluble nitrogen (TSN) and total nitrogen (TN) were 2, 5 and 10 KGy, respectively. Such effects were proportionally related to the applied dose. The loss in nitrogen compounds and/or their solubility which occurred upon irradiation appeared to be retarded by soaking treatment. Irradiation treatments induced additional protein fraction which seems to be originated from the sarcoplasmic proteins. After the resolution of rigor mortis, the incremental rate of nitrogen extractability was inversely related to the irradiation dose. Another protein fraction was detected only in the 10 KGy irradiated samples after 14 days of cold storage which might be originating from fibrillar proteins as a result of its interaction with some lipid oxidation products. However, soaking treatment itself caused extensive changes in protein fractions, in contrast, protection from radiation and radiation after-effects were observed

  4. Metabolic encephalopathy and lipid storage myopathy associated with a presumptive mitochondrial fatty acid oxidation defect in a dog

    Directory of Open Access Journals (Sweden)

    Vanessa R Biegen

    2015-11-01

    Full Text Available A 1-year-old spayed female Shih Tzu presented for episodic abnormalities of posture and mentation. Neurologic examination was consistent with a bilaterally symmetric multifocal encephalopathy. The dog had a waxing-and-waning hyperlactemia and hypoglycemia. Magnetic resonance imaging revealed bilaterally symmetric cavitated lesions of the caudate nuclei with less severe abnormalities in the cerebellar nuclei. Empirical therapy was unsuccessful and the patient was euthanized. Post-mortem histopathology revealed bilaterally symmetric necrotic lesions of the caudate and cerebellar nuclei and multi-organ lipid accumulation, including a lipid storage myopathy. Malonic aciduria and ketonuria were found on urinary organic acid screen. Plasma acylcarnitine analysis suggested a fatty acid oxidation defect. Fatty acid oxidation disorders are inborn errors of metabolism documented in humans, but poorly described in dogs. Although neurologic signs have been described in humans with this group of diseases, descriptions of advanced imaging and histopathology are severely lacking. This report suggests that abnormalities of fatty acid metabolism may cause severe, bilateral gray matter necrosis and lipid accumulation in multiple organs including the skeletal muscles, liver, and kidneys. Veterinarians should be aware that fatty acid oxidation disorders, although potentially fatal, may be treatable. A timely definitive diagnosis is essential in guiding therapy.

  5. Studies on RBC lipid and protein phosphorylation during blood bank storage

    International Nuclear Information System (INIS)

    Dumaswala, U.J.; Bryan, D.J.; Greenwalt, T.J.

    1986-01-01

    Recent evidence has suggested that phosphoinositides play a significant role in maintaining membrane structure and function. Their importance during blood bank storage is not understood. They have performed preliminary studies of the phosphoinositide synthetic pathway enzymes of RBC during blood bank storage. At 0 and 35 days of storage leaky ghosts were prepared and incubated with [γ- 32 P]ATP for 5 minutes at 30 C. One aliquot was subjected to acidified solvent extraction and thin layer chromatography. The labeled phosphoinositide -4,5 biphosphate (PIP 2 ), phosphoinositide-4 phosphate (PIP) and phosphatidic acid (PA) spots were scraped and counted by liquid scintillation spectrometry. Another aliquot was used for SDS-PAGE and the radioactivity associated with the β-spectrin was measured. These experiments suggest a decrease in RBC phosphoinositol and PIP-Kinases and β-spectrin kinase activities during blood bank storage. Further studies are being done to evaluate significance of these observations

  6. Germinação e armazenamento de sementes de Camboatã (Cupania vernalis Cambess. sapindaceae Germination and storage of Cupania vernalis Cambess. seeds - sapindaceae

    Directory of Open Access Journals (Sweden)

    Carlos Vinício Vieira

    2008-04-01

    Full Text Available Sementes de Cupania vernalis Cambess. possuem características recalcitrantes quando submetidas à secagem, além de curta longevidade, necessitando da aplicação de conhecimentos relacionados a fatores que interferem no armazenamento. Objetivou-se com esta pesquisa avaliar as variações fisiológicas de sementes de C. vernalis quando submetidas a diferentes temperaturas e teores de água, durante o armazenamento. No armazenamento, sementes com graus de umidade de 40%, 35% e 30% foram acondicionadas em sacos plásticos e armazenadas sob temperatura controlada de 10ºC e 25ºC, com umidade relativa de 60%, avaliadas quanto ao grau de umidade, porcentagem de germinação e índice de velocidade de emergência, nos tempos de 0, 120 e 240 dias. O tratamento em que as sementes continham 40% de grau de umidade e armazenadas a temperatura de 10ºC favorece a conservação das sementes de C. vernalis.Cupania vernalis Cambess. seeds have a short longevity with a recalcitrant behavior when submitted to drying. This demands development and adoption of technologies to improve storage and conservation of the seeds. Thus, the objective of this work was to evaluate physiological aspects of Cupania vernalis seeds at different storage temperatures and to different seed moisture content. Seeds with 40%, 35% and 30% of water content were conditioned in plastic bags and stored at 10ºC and 25ºC under controlled temperature and relative humidity around of 60%. During 0, 120 and 240 days of storage seeds were taken for evaluation of water content, germination, emergency index. The treatments in which seed contained moisture content of 40% and stored at temperature of 10ºC helps the conservation of seeds of C. vernalis.

  7. Effect of microwave irradiation on germination and seedling growth physiological characteristics of alfalfa seeds after storage

    International Nuclear Information System (INIS)

    Chen Liyu; Zhang Shuqing; Li Jianfeng; Shi Shangli; Huo Pinghui

    2012-01-01

    In order to study the effect of microwave irradiation on germination and growth physiological characteristics of seeds that stored for years, the irradiated alfalfa seeds that stored at room temperature for 2 years were used to conduct the germination and pot culture tests, and the germination rate, radical elongation, growth height, individual nodule, nitrogenase activity, chlorophyll content and chlorophyll fluorescence parameters were measured. On the 15th day of germination, the germination rates of all the treatments are higher than that of the control, which decrease with the elongation of time. On the llst day of germination, the radical length of all the treatments is lower than that of the control. Growth height, individual nodule, fresh weight and dry weight for the 40 s irradiation treatment are higher than that of the control. Nitrogenase activity of all the treatments is lower than that of the control (P < O.05). The chlorophyll content reaches its maximum when being irradiated for 10 s, and the variation for F 0 and F v /F m of all treatments indicates that the light conversion efficiency of the leaves derived from the irradiated alfalfa seeds that stored for 2 a at room temperature is still relatively stressed. (authors)

  8. Antioxidative and prooxidative effects in food lipids and synergism with α-tocopherol of açaí seed extracts and grape rachis extracts

    DEFF Research Database (Denmark)

    Melo, Priscilla Siqueira; Arrivetti, Leandro de Oliveira Rodrigues; de Alencar, Severino Matias

    2016-01-01

    Extracts of açaí seed and of grape rachis alone or in combination with α-tocopherol were evaluated as antioxidants in (i) bulk soybean oil, (ii) soybean oil liposomes and (iii) soybean-oil/water emulsions. The extracts made with 57% aqueous ethanol showed an antioxidant activity not dependent...... on concentration for grape rachis extracts and a concentration-dependent prooxidative activity for açaí seed extracts in bulk soybean oil. Both the extracts, however, protected liposome suspensions and oil/water emulsions against lipid oxidation. Synergism was demonstrated when extracts were combined with α......-tocopherol, effects explained by the solubility of extract components in the water-phase and of α-tocopherol in the lipid-phase. Phenolic profiling of the extracts by U-HPLC-ESI-LTQ-MS was used to identify active antioxidants. Açaí seed and grape rachis extracts served as good sources of procyanidins and flavan-3-ols...

  9. The effect of pumpkin (Cucurbita pepo L) seeds and L-arginine supplementation on serum lipid concentrations in atherogenic rats.

    Science.gov (United States)

    Abuelgassim, Abuelgassim O; Al-showayman, Showayman I A

    2012-01-01

    The present study aimed to examine the effect of pumpkin (Cucurbita pepo L.) seeds supplementation on atherogenic diet-induced atherosclerosis. Rat were divided into two main groups , normal control and atherogenic control rats , each group composed of three subgroups one of them supplemented with 2% arginine in drinking water and the other supplemented with pumpkin seeds in diet at a concentration equivalent to 2% arginine. Supplementation continued for 37 days. Atherogenic rats supplemented with pumpkin seeds showed a significant decrease (ppumpkin seeds significantly decreased serum concentrations of TC and LDL-C. Our findings suggest that pumpkin seeds supplementation has a protective effect against atherogenic rats and this protective effect was not attributed to the high arginine concentrations in pumpkin seeds.

  10. Pharmacognostic and phytochemical analyses of leaves and seed storage of abutilon pakistanicum jafri and ali an endemic plant of pakistan

    International Nuclear Information System (INIS)

    Fatima, H.; Munir, U.

    2014-01-01

    There are vast varieties of medicinal plant in the world having therapeutical importance. With increasing popularity of herbal medicine as a curative measure, the need for correct identification and standardization of the plant is also increased. Present work was performed to study the pharmacognostic and phytochemical characters of leaves and exsitu seed conservation by seed storage of Abutilon pakistanicum Jafri and Ali. It is an endemic plant, found in the sub tropical regions of Pakistan and is used in traditional medicine for treating rheumatism. The leaves of Abutilon pakistanicum Jafri andAli were investigated for pharmacognostic parameters. Phytochemical screening, macroscopic characters, physiochemical attributes and fluorescence analysis. The results revealed the presence of pharmacologically active compounds like alkaloids, flavonoids, terpinoids, saponins and phenolic compounds, leaf constant values and the extractive values obtained were found close to the values reported for other Abutilon species, while high ash value indicated presence of impurities in the crude drug. The investigation provides information for correct identification and authentication of plant species for further studies and medicinal evaluation of the species. (author)

  11. Combined effect of cooking (grilling and roasting) and chilling storage (with and without air) on lipid and cholesterol oxidation in chicken breast.

    Science.gov (United States)

    Conchillo, Ana; Ansorena, Diana; Astiasarán, Iciar

    2003-05-01

    The oxidation of the lipid fraction and cholesterol in raw and cooked chicken breast samples stored for 0 and 6 days at 4 degrees C under aerobic conditions and in vacuum packaging was studied. The multivariate statistical analysis showed significant effects of both culinary process and storage conditions on the lipid and cholesterol oxidation process, with a significant interaction between the two variables. Aerobic storage increased thiobarbituric acid reactive substances (TBA) from 0.04 to 0.06 ppm for raw samples, from 0.21 to 1.20 ppm for grilled samples, and from 0.24 to 1.62 ppm for roasted samples. During vacuum storage, only roasted samples showed significant increases in TBA. Levels of total cholesterol oxidation products (COP) remained low (2.88 to 4.35 microg/g of lipid) for all raw samples. Cooking increased COP levels to 12.85 and 11.54 microg/ g of lipid for grilled and roasted samples, respectively. Total COP and all individual COP except for cholestanetriol were significantly correlated with TBA and the peroxide index. However, the most extensive effect was attributable to the aerobic storage of cooked samples, which led to COP levels of 92.35 and 88.60 microg/g of lipid in grilled and roasted samples, respectively. Vacuum packaging did not increase COP levels for cooked samples.

  12. Purification and partial characterization of storage proteins in Lupinus angustifolius seeds

    Directory of Open Access Journals (Sweden)

    Millán, Francisco

    2004-12-01

    Full Text Available Lupinus angustifolius seed proteins have been purified by sequential dialysis and ion exchange chromatography, and their amino acid composition has been studied in order to determine their nutritional value as sources of essential amino acids. Albumins include a great variety of proteins. Globulins were resolved in α, β and δ conglutins. Conglutin α is the main protein in the seeds of L. angustifolius, representing 76.6% of the total. While lysine was found to be the limiting amino acid in L. Angustifolius seed proteins as a whole, tyrosine was the limiting amino acid in albumins, and methione and lysine were limiting in globulins. Lysine, methionine and histidine were limiting amino acids in  α conglutin.Se ha realizado una purificación por diálisis secuencial y cromatografía de intercambio iónico de las proteínas de la semilla de L. angustifolius, y su composición en aminoácidos ha sido estudiada para determinar su valor nutricional como fuentes de aminoácidos esenciales. Las albúminas están compuestas por una compleja mezcla de proteínas. Las globulinas se resolvieron en conglutinas α, β y δ. La conglutina  α es la proteína más abundante en las semillas de L. angustifolius representando el 76.6% del total. Las proteínas de la semilla de L. angustifolius son limitantes en lisina. Las albúminas son limitantes en tirosina y las globulinas en metionina y lisina. La α conglutina es limitante en lisina, metionina e histidina.

  13. Proteomic analysis of seed storage proteins in wild rice species of the Oryza genus.

    Science.gov (United States)

    Jiang, Chunmiao; Cheng, Zaiquan; Zhang, Cheng; Yu, Tengqiong; Zhong, Qiaofang; Shen, J Qingxi; Huang, Xingqi

    2014-01-01

    The total protein contents of rice seeds are significantly higher in the three wild rice species (Oryza rufipogon Grill., Oryza officinalis Wall. and Oryza meyeriana Baill.) than in the cultivated rice (Oryza sativa L.). However, there is still no report regarding a systematic proteomic analysis of seed proteins in the wild rice species. Also, the relationship between the contents of seed total proteins and rice nutritional quality has not been thoroughly investigated. The total seed protein contents, especially the glutelin contents, of the three wild rice species were higher than those of the two cultivated rice materials. Based on the protein banding patterns of SDS-PAGE, O. rufipogon was similar to the two cultivated rice materials, followed by O. officinalis, while O. meyeriana exhibited notable differences. Interestingly, O. meyeriana had high contents of glutelin and low contents of prolamine, and lacked 26 kDa globulin band and appeared a new 28 kDa protein band. However, for O. officinali a 16 kDa protein band was absent and a row of unique 32 kDa proteins appeared. In addition, we found that 13 kDa prolamine band disappeared while special 14 kDa and 12 kDa protein bands were present in O. officinalis. Two-dimensional gel electrophoresis (2-DE) analysis revealed remarkable differences in protein profiles of the wild rice species and the two cultivated rice materials. Also, the numbers of detected protein spots of the three wild rice species were significantly higher than those of two cultivated rice. A total of 35 differential protein spots were found for glutelin acidic subunits, glutelin precursors and glutelin basic subunits in wild rice species. Among those, 18 protein spots were specific and 17 major spots were elevated. Six differential protein spots for glutelin acidic subunits were identified, including a glutelin type-A 2 precursor and five hypothetical proteins. This was the first report on proteomic analysis of the three wild rice species

  14. Lysosome lipid storage disorder in NCTR-BALB/c mice. II. Morphologic and cytochemical studies.

    OpenAIRE

    Shio, H.; Fowler, S.; Bhuvaneswaran, C.; Morris, M. D.

    1982-01-01

    Electron-microscopic and cytochemical studies were carried out on tissues of NCTR-BALB/c mice. These mice are affected with a neurovisceral genetic disorder involving excessive tissue accumulation of lipid. Distinctive polymorphic intracellular inclusions, bounded by a membrane and containing lamellated bodies, were found in many cells of liver, spleen, lung, kidney, intestine, lymph nodes, and brain. The inclusions transformed reticuloendothelial cells into massive foam cells. Acid phosphata...

  15. Effect of bioactive substances found in rapeseed, raspberry and strawberry seed oils on blood lipid profile and selected parameters of oxidative status in rats.

    Science.gov (United States)

    Pieszka, Marek; Tombarkiewicz, Barbara; Roman, Adam; Migdał, Władysław; Niedziółka, Jerzy

    2013-11-01

    Rapeseed, strawberry and raspberry seed oils are a rich source of polyunsaturated fatty acids and antioxidants such as tocols, bioflavonoids and phytosterols. The aim of the study was to determine changes in the blood lipid profile of rats fed with rapeseed, strawberry and raspberry seed oils and their effects on selected parameters of oxidative status. The experiment was carried out on male Wistar rats. The oils were administered by oral gavage for 5 weeks once daily at the dose of about 0.8 ml per rat. Blood samples were taken before and after supplementation period. The activity of superoxide dismutase (SOD) and glutathione peroxidase (cGPx) was assessed in erythrocytes and contents of triglycerides (TG), total cholesterol, low-density fraction of cholesterol (LDL) and high-density fraction of cholesterol (HDL) were assessed in plasma. The experiment shows that oils supplemented in the diet for 5 weeks had no significant effect on the level of triglyceride (TG), total cholesterol as well as HDL and LDL fractions. Reduced activity of cGPX and SOD in the group of rats receiving raspberry and strawberry seed oils suggests that these native oils may contribute to oxidative stability (improves antioxidant status). Thus, strawberry and raspberry seed oils can be considered as special biological oils, which constitute potential nutraceuticals reducing oxidative stress. Copyright © 2013 Elsevier B.V. All rights reserved.

  16. Transcriptome analysis of pecan seeds at different developing stages and identification of key genes involved in lipid metabolism.

    Science.gov (United States)

    Xu, Zheng; Ni, Jun; Shah, Faheem Afzal; Wang, Qiaojian; Wang, Zhaocheng; Wu, Lifang; Fu, Songling

    2018-01-01

    Pecan is an economically important nut crop tree due to its unique texture and flavor properties. The pecan seed is rich of unsaturated fatty acid and protein. However, little is known about the molecular mechanisms of the biosynthesis of fatty acids in the developing seeds. In this study, transcriptome sequencing of the developing seeds was performed using Illumina sequencing technology. Pecan seed embryos at different developmental stages were collected and sequenced. The transcriptomes of pecan seeds at two key developing stages (PA, the initial stage and PS, the fast oil accumulation stage) were also compared. A total of 82,155 unigenes, with an average length of 1,198 bp from seven independent libraries were generated. After functional annotations, we detected approximately 55,854 CDS, among which, 2,807 were Transcription Factor (TF) coding unigenes. Further, there were 13,325 unigenes that showed a 2-fold or greater expression difference between the two groups of libraries (two developmental stages). After transcriptome analysis, we identified abundant unigenes that could be involved in fatty acid biosynthesis, degradation and some other aspects of seed development in pecan. This study presents a comprehensive dataset of transcriptomic changes during the seed development of pecan. It provides insights in understanding the molecular mechanisms responsible for fatty acid biosynthesis in the seed development. The identification of functional genes will also be useful for the molecular breeding work of pecan.

  17. Stability of selected serum hormones and lipids after long-term storage in the Janus Serum Bank.

    Science.gov (United States)

    Gislefoss, Randi E; Grimsrud, Tom K; Mørkrid, Lars

    2015-04-01

    The potential value of a biobank depends on the quality of the samples, i.e. how well they reflect the biological or biochemical state of the donors at the time of sampling. Documentation of sample quality has become a particularly important issue for researchers and users of biobank studies. The aim of this study was to investigate the long-term stability of selected components: cholesterol, high density cholesterol (HDLC), low density cholesterol (LDLC), apolipoprotein A1 (apo-A1), apolipoprotein B (apo B), follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), thyroid stimulating hormone (TSH) and free thyroxin (FT4). Samples, stored at -25°C, from 520 men aged 40-49 years at blood sampling distributed in equally sized groups (n=130) according to length of storage, 0, 4, 17 and 29 years, respectively, were used in a cross sectional design. The freshly collected serum samples were used as a reference group to calculate storage related changes. The differences between fresh samples and samples stored for 29 years were substantial for apo-A1 (+12%), apo-B (+22.3%), HDLC (-69.2%), LDLC (+31.3%), and PRL (-33.5%), while total cholesterol, FSH, LH, TSH and FT4 did not show any significant difference. The study showed large differences in serum level of the selected components. The lipids and apolipoproteins were all changed except for total cholesterol. Most hormones investigated (FSH, LH, TSH and FT4) proved to be stable after 29 years of storage while PRL showed sign of degradation. The observed differences are probably due to long-term storage effects and/or external factors (i.e. diet and smoking). Copyright © 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  18. Degradation rates of phorbol esters in Jatropha curcas L. oil and pressed seeds under different storage conditions.

    Science.gov (United States)

    Phasukarratchai, Naphatsarnan; Damrongsiri, Seelawut; Tongcumpou, Chantra

    2017-03-01

    Phorbol esters (PEs), found in Jatropha curcas crude oil (JCO) and J. curcas pressed seeds (JPS), are known as bioactive compounds in agricultural and pharmaceutical applications. The degradation rates of PEs in JCO and JPS under various conditions is important for the utilisation of PEs. Thus the objective of this study was to determine the PE degradation rates in JCO and JPS under different storage conditions. PE degradation rates were found to be first-order reactions. The slowest degradation rate was at 0.9 × 10 -3 d -1 for both JCO and JPS unexposed to light at 4 °C. Light intensity (1097 lx and 4690 lx, representing diffused sunlight and fluorescent lighting, respectively) and temperature (25 to 35 °C) were the significant degradation factors. Light exposure led to 280% to 380% higher degradation rates in JCO than in JPS due to light penetration through the transparent oil. Dried and sterilised JPS showed an 80% to 90% lower PE degradation rate than untreated JPS under all storage conditions since biodegradation was assembly limited. The PEs were unstable under the studied conditions, especially when exposed to light and room temperature. To protect against PE degradation, a material should be stored in a light-protected container and below 4 °C. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  19. Restarting stalled autophagy a potential therapeutic approach for the lipid storage disorder, Niemann-Pick type C1 disease.

    Science.gov (United States)

    Sarkar, Sovan; Maetzel, Dorothea; Korolchuk, Viktor I; Jaenisch, Rudolf

    2014-06-01

    Autophagy is essential for cellular homeostasis and its dysfunction in human diseases has been implicated in the accumulation of misfolded protein and in cellular toxicity. We have recently shown impairment in autophagic flux in the lipid storage disorder, Niemann-Pick type C1 (NPC1) disease associated with abnormal cholesterol sequestration, where maturation of autophagosomes is impaired due to defective amphisome formation caused by failure in SNARE machinery. Abrogation of autophagy also causes cholesterol accumulation, suggesting that defective autophagic flux in NPC1 disease may act as a primary causative factor not only by imparting its deleterious effects, but also by increasing cholesterol load. However, cholesterol depletion treatment with HP-β-cyclodextrin impedes autophagy, whereas pharmacologically stimulating autophagy restores its function independent of amphisome formation. Of potential therapeutic relevance is that a low dose of HP-β-cyclodextrin that does not perturb autophagy, coupled with an autophagy inducer, may rescue both the cholesterol and autophagy defects in NPC1 disease.

  20. Muscle MRI in neutral lipid storage disease with myopathy carrying mutation c.187+1G>A.

    Science.gov (United States)

    Xu, Chunxiao; Zhao, Yawen; Liu, Jing; Zhang, Wei; Wang, Zhaoxia; Yuan, Yun

    2015-06-01

    We describe the clinical and muscle MRI changes in 2 siblings with neutral lipid storage disease with myopathy (NLSDM) carrying the mutation c.187+1G>A. Peripheral blood smears, genetic tests, and muscle biopsies were performed. Thigh MRI was performed to observe fatty replacement, muscle edema, and muscle bulk from axial sections. Both siblings had similar fatty infiltration and edema. T1-weighted images of the gluteus maximus, adductor magnus, semitendinosus, and semimembranosus revealed marked and diffuse fatty infiltration. There was asymmetric involvement in biceps femoris and quadriceps. There was extensive fatty infiltration in the quadriceps, except for the rectus femoris. Gracilis and sartorius were relatively spared. Thigh muscle volume was decreased, while the gracilis and sartorius appeared to show compensatory hypertrophy. Compared with previous reports in NLSDM, MRI changes in this myopathy tended to be more severe. Asymmetry and relatively selective fatty infiltration were characteristics. © 2014 Wiley Periodicals, Inc.

  1. Rheology, fatty acid profile and storage characteristics of cookies as influenced by flax seed (Linum usitatissimum).

    Science.gov (United States)

    Rajiv, Jyotsna; Indrani, Dasappa; Prabhasankar, Pichan; Rao, G Venkateswara

    2012-10-01

    Flaxseed is a versatile functional ingredient owing to its unique nutrient profile. Studies on the effect of substitution of roasted and ground flaxseed (RGF) at 5, 10, 15 and 20% level on the wheat flour dough properties showed that amylograph peak viscosity, farinograph dough stability, extensograph resistance to extension and extensibility values decreased with the increase in the substitution of RGF from 0-20%. The cookie baking test showed a marginal decrease in spread ratio but beyond substitution of 15% RGF the texture and flavour of the cookies was adversely affected. The data on storage characteristics of control and cookies with 15% RGF showed no significant change with respect to acidity of extracted fat and peroxide values due to storage of cookies upto 90 days in metallised polyester pouches at ambient conditions. The gas chromatographic analysis of fatty acid profile indicated that the control cookies contained negligible linolenic acid and the flaxseed cookies contained 4.75 to 5.31% of linolenic acid which showed a marginal decrease over storage. Hence flaxseed could be used as a source of omega-3-fatty acid.

  2. Molecular and biochemical characterization in Rauvolfia tetraphylla plantlets grown from synthetic seeds following in vitro cold storage.

    Science.gov (United States)

    Faisal, Mohammad; Alatar, Abdularhaman A; Hegazy, Ahmad K

    2013-01-01

    Synseed technology is one of the most important applications of plant biotechnology for in vitro conservation and regeneration of medicinal and aromatic plants. In the present investigation, synseeds of Rauvolfia tetraphylla were produced using in vitro-proliferated shoots upon complexation of 3 % sodium alginate and 100 mM CaCl(2). The encapsulated buds were stored at 4, 8, 12, and 16 °C and high conversion was observed in synseeds stored at 4 °C for 4 weeks. The effect of different medium strength on in vitro conversion response of synseed was evaluated and the maximum conversion (80.6 %) into plantlets was recorded on half-strength woody plant medium supplemented with 7.5 μM 6-benzyladenine and 2.5 μM α-naphthalene acetic acid after 8 weeks of culture. Plantlets with well-developed shoot and roots were hardened and successfully transplanted in field condition. After 4 weeks of transfer to ex vitro conditions, the performance of synseed-derived plantlets was evaluated on the basis of some physiological and biochemical parameters and compared with the in vivo-grown plants. Short-term storage of synthetic seeds at low temperature had no negative impact on physiological and biochemical profile of the plants that survived the storage process. Furthermore, clonal fidelity of synseed-derived plantlets was also assessed and compared with mother plant using rapid amplified polymorphic DNA and inter-simple sequence repeats analysis. No changes in molecular profiles were found among the regenerated plantlets and comparable to mother plant, which confirm the genetic stability among clones. This synseed protocol could be useful for in vitro clonal multiplication, conservation, and short-term storage and exchange of germplasm of this antihypertensive drug-producing plant.

  3. PEG-lipid micelles enable cholesterol efflux in Niemann-Pick Type C1 disease-based lysosomal storage disorder

    Science.gov (United States)

    Brown, Anna; Patel, Siddharth; Ward, Carl; Lorenz, Anna; Ortiz, Mauren; Duross, Allison; Wieghardt, Fabian; Esch, Amanda; Otten, Elsje G.; Heiser, Laura M.; Korolchuk, Viktor I.; Sun, Conroy; Sarkar, Sovan; Sahay, Gaurav

    2016-08-01

    2-Hydroxy-propyl-β-cyclodextrin (HPβCD), a cholesterol scavenger, is currently undergoing Phase 2b/3 clinical trial for treatment of Niemann Pick Type C-1 (NPC1), a fatal neurodegenerative disorder that stems from abnormal cholesterol accumulation in the endo/lysosomes. Unfortunately, the extremely high doses of HPβCD required to prevent progressive neurodegeneration exacerbates ototoxicity, pulmonary toxicity and autophagy-based cellular defects. We present unexpected evidence that a poly (ethylene glycol) (PEG)-lipid conjugate enables cholesterol clearance from endo/lysosomes of Npc1 mutant (Npc1-/-) cells. Herein, we show that distearyl-phosphatidylethanolamine-PEG (DSPE-PEG), which forms 12-nm micelles above the critical micelle concentration, accumulates heavily inside cholesterol-rich late endosomes in Npc1-/- cells. This potentially results in cholesterol solubilization and leakage from lysosomes. High-throughput screening revealed that DSPE-PEG, in combination with HPβCD, acts synergistically to efflux cholesterol without significantly aggravating autophagy defects. These well-known excipients can be used as admixtures to treat NPC1 disorder. Increasing PEG chain lengths from 350 Da-30 kDa in DSPE-PEG micelles, or increasing DSPE-PEG content in an array of liposomes packaged with HPβCD, improved cholesterol egress, while Pluronic block copolymers capable of micelle formation showed slight effects at high concentrations. We postulate that PEG-lipid based nanocarriers can serve as bioactive drug delivery systems for effective treatment of lysosomal storage disorders.

  4. The perilipin homologue, lipid storage droplet 2, regulates sleep homeostasis and prevents learning impairments following sleep loss.

    Directory of Open Access Journals (Sweden)

    Matthew S Thimgan

    2010-08-01

    Full Text Available Extended periods of waking result in physiological impairments in humans, rats, and flies. Sleep homeostasis, the increase in sleep observed following sleep loss, is believed to counter the negative effects of prolonged waking by restoring vital biological processes that are degraded during sleep deprivation. Sleep homeostasis, as with other behaviors, is influenced by both genes and environment. We report here that during periods of starvation, flies remain spontaneously awake but, in contrast to sleep deprivation, do not accrue any of the negative consequences of prolonged waking. Specifically, the homeostatic response and learning impairments that are a characteristic of sleep loss are not observed following prolonged waking induced by starvation. Recently, two genes, brummer (bmm and Lipid storage droplet 2 (Lsd2, have been shown to modulate the response to starvation. bmm mutants have excess fat and are resistant to starvation, whereas Lsd2 mutants are lean and sensitive to starvation. Thus, we hypothesized that bmm and Lsd2 may play a role in sleep regulation. Indeed, bmm mutant flies display a large homeostatic response following sleep deprivation. In contrast, Lsd2 mutant flies, which phenocopy aspects of starvation as measured by low triglyceride stores, do not exhibit a homeostatic response following sleep loss. Importantly, Lsd2 mutant flies are not learning impaired after sleep deprivation. These results provide the first genetic evidence, to our knowledge, that lipid metabolism plays an important role in regulating the homeostatic response and can protect against neuronal impairments induced by prolonged waking.

  5. SDS-Page Seed Storage Protein Profiles in Chili Peppers (Capsicum L.

    Directory of Open Access Journals (Sweden)

    Owk ANIEL KUMAR

    2010-09-01

    Full Text Available Seed protein banding patterns (SDS-PAGE were studied from eighteen genotypes of chili pepper (Capsicum L. A total of 21 protein polypeptide bands with molecular weight ranging from 18.6 to 72.0 kD were recorded. Among the genotypes CA18, CA21 and CA27 represented maximum number of protein bands (12. Band no. (11 and (5,12 are exclusive to C. annuum L. and C. frutescens L. genotypes respectively. Average percent similarity was highest (100% between CA2 and CA8 genotypes and the UPGMA dendrogram represented low genetic diversity. The study revealed that considerable intra and inter-specific differences were found in the genotypes. The variability of protein profiles in the genotypes suggested that these selected genotypes can be a good source for crop improvement through hybridization programs.

  6. Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo

    Czech Academy of Sciences Publication Activity Database

    Samek, Ota; Jonáš, Alexandr; Pilát, Zdeněk; Zemánek, Pavel; Nedbal, Ladislav; Tříska, Jan; Kotas, Petr; Trtílek, M.

    2010-01-01

    Roč. 10, č. 9 (2010), s. 8635-8651 ISSN 1424-8220 R&D Projects: GA MŠk ED0017/01/01; GA MŠk OC08034; GA MPO FR-TI1/433; GA ČR GA206/09/1284 Grant - others:EU(XE) PERG 06-GA-2009-256526 Institutional research plan: CEZ:AV0Z20650511; CEZ:AV0Z60870520 Keywords : Raman spectroscopy * algal cells * lipids * iodine value Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 1.771, year: 2010

  7. Biomarkers in the diagnosis of lysosomal storage disorders: proteins, lipids, and inhibodies.

    Science.gov (United States)

    Aerts, Johannes M F G; Kallemeijn, Wouter W; Wegdam, Wouter; Joao Ferraz, Maria; van Breemen, Marielle J; Dekker, Nick; Kramer, Gertjan; Poorthuis, Ben J; Groener, Johanna E M; Cox-Brinkman, Josanne; Rombach, Saskia M; Hollak, Carla E M; Linthorst, Gabor E; Witte, Martin D; Gold, Henrik; van der Marel, Gijs A; Overkleeft, Herman S; Boot, Rolf G

    2011-06-01

    A biomarker is an analyte indicating the presence of a biological process linked to the clinical manifestations and outcome of a particular disease. In the case of lysosomal storage disorders (LSDs), primary and secondary accumulating metabolites or proteins specifically secreted by storage cells are good candidates for biomarkers. Clinical applications of biomarkers are found in improved diagnosis, monitoring disease progression, and assessing therapeutic correction. These are illustrated by reviewing the discovery and use of biomarkers for Gaucher disease and Fabry disease. In addition, recently developed chemical tools allowing specific visualization of enzymatically active lysosomal glucocerebrosidase are described. Such probes, coined inhibodies, offer entirely new possibilities for more sophisticated molecular diagnosis, enzyme replacement therapy monitoring, and fundamental research.

  8. Development of functional cookies with wheat flour, banana flour (Musa paradisiaca, sesame seeds (Sesamum indicum and storage stability

    Directory of Open Access Journals (Sweden)

    Angélica Loza

    2017-01-01

    Full Text Available Functional cookies were developed using banana flour (BF and sesame seeds (SS. Protein, moisture and ash w ere determined , and farinographic analyzes of flours were performed. The attributes odor, color, flavor, crunch and the IC 50 value of the cookies were determined. The results were evaluated with the C omplete R andomized D esign and the Tukey and Kruskall Wallis test. The flour mixture presented higher protein (10.2%, humidity (14.40 % than BF, but lower than wheat flour. C ookies with 10 % , 15 % and 20% BF and 8% sesame seeds were selected. Flours with 10 % , 15 % and 20% BF had similar values of water absorption (≤ 60% and different values statistically (p ≤ 0.05 for development time, mass stability and degree of softening. Cook ies with 20% BF and 8% SS (SC had IC 50 = 17.52 ± 0.25 mg / mL, with moisture, protein, fat, crude fiber, ash and carbohydrates of 1.88 % , 10.65 % , 22.01 % , 1.01 % , 1.54 % and 62.91%, respectively. SC did not present sensorial statistical differences (p ≤ 0.05 the first two months, the third month decreased the acceptability of the crunch and flavor. In ninety days of storage the IC50 value (29.07 ± 0.92 mg / mL, reducing sugars (1.20 ± 0.02 and pH (5.24 ± 0.01 decreased and humidity (3.83 ± 0.03 increased.

  9. Proteomic analysis of lettuce seed germination and thermoinhibition by sampling of individual seeds at germination and removal of storage proteins by polyethylene glycol fractionation

    DEFF Research Database (Denmark)

    Wang, Wei-Qing; Song, Bin-Yan; Deng, Zhi-Jun

    2015-01-01

    the sensitivity of germination to abscisic acid. MVA pathway-derived products, cytokinins, partially reversed the lovastatin inhibition of germination and released seed thermoinhibition at 25°C. We conclude that the MVA pathway for isoprenoid biosynthesis is involved in lettuce seed germination...

  10. Effects of diet, packaging, and irradiation on protein oxidation, lipid oxidation, and color of raw broiler thigh meat during refrigerated storage.

    Science.gov (United States)

    Xiao, S; Zhang, W G; Lee, E J; Ma, C W; Ahn, D U

    2011-06-01

    This study was designed to evaluate the effects of dietary treatment, packaging, and irradiation singly or in combination on the oxidative stability of broiler chicken thigh meat. A total of 120 four-week-old chickens were divided into 12 pens (10 birds/pen), and 4 pens of broilers were randomly assigned to a control oxidized diet (5% oxidized oil) or an antioxidant-added diet [500 IU of vitamin E + 200 mg/kg of butylated hydroxyanisole (BHA)] and fed for 2 wk. After slaughter, thigh meats were separated, ground, packaged in either oxygen-permeable or oxygen-impermeable vacuum bags, and irradiated at 0 or 3 kGy. Lipid oxidation (TBA-reactive substances), protein oxidation (carbonyl), and color of the meat were measured at 1, 4, and 7 d of refrigerated storage. The lipid and protein oxidation of thigh meats from birds fed the diet supplemented with antioxidants (vitamin E + BHA) was significantly lower than the lipid and protein oxidation of birds fed the control diet, whereas the lipid and protein oxidation of broilers fed the oxidized oil diet was higher than that of birds fed the control diet. Vacuum packaging slowed, but irradiation accelerated, the lipid and protein oxidation of thigh meat during storage. Dietary antioxidants (vitamin E + BHA) and irradiation treatments showed a stronger effect on lipid oxidation than on protein oxidation. A significant correlation between lipid and protein oxidation in meat was found during storage. Dietary supplementation of vitamin E + BHA and the irradiation treatment increased the lightness and redness of thigh meat, respectively. It is suggested that appropriate use of dietary antioxidants in combination with packaging could be effective in minimizing oxidative changes in irradiated raw chicken thigh meat.

  11. Dehydration-induced redistribution of amphiphilic molecules between cytoplasm and lipids is associated with desiccation tolerance in seeds

    NARCIS (Netherlands)

    Buitink, J.; Leprince, O.; Hoekstra, F.A.

    2000-01-01

    This study establishes a relationship between desiccation tolerance and the transfer of amphiphilic molecules from the cytoplasm into lipids during drying, using electron paramagnetic resonance spectroscopy of amphiphilic spin probes introduced into imbibed radicles of pea (Pisum sativum) and

  12. Developing eco-friendly biofungicide for the management of major seed borne diseases of rice and assessing their physical stability and storage life.

    Science.gov (United States)

    Naveenkumar, Ramasamy; Muthukumar, Arjunan; Sangeetha, Ganesan; Mohanapriya, Ramanathan

    2017-04-01

    Three plant oils (Cymbopogon citratus, Cymbopogon martini, and Pelargonium graveolens) were developed as EC formulations and tested for their physical stabilities. EC formulations (10EC, 20EC and 30EC) of C. citratus, C. martini and P. graveolens had emulsion stability, spontaneity property, heat and cold stability. EC formulated plant oils were screened against the major seed borne fungi of rice such as Curvularia lunata, Fusarium moniliforme, Bipolaris oryzae, and Sarocladium oryzae. The level of inhibition varied among the concentrations of EC formulations. Among the three EC formulations, that of C. citratus oil 30EC recorded 100% inhibition on the mycelial growth of test pathogens. In the blotter paper method, rice seeds treated with a formulation of C. citratus oil 30EC controlled the infection of C. lunata, F. moniliforme, B. oryzae and S. oryzae in rice seed to the tune of 66.0%, 60.4%, 66.0% and 69.1%, respectively. Seed soaking with formulation of C. citratus oil 30EC showed the highest percentage of normal seedlings, the lowest number of abnormal seedling and fresh ungerminated seeds when tested with the roll-towel method. Seed soaking with 30EC formulation of C. citratus oil increased seed germination, shoot length, root length and vigour of rice seedlings when tested with the plastic tray method. Transmission of pathogens from seed to seedling was reduced significantly by the 30EC formulation of C. citratus oil when tested with the plastic pot method. The effect of the storage life of the 30EC formulation of C. citratus oil showed that it had retained their antifungal effect till the end of the incubation period (120 days), and is able to inhibit the mycelial growth of all test pathogens to the 100% level. Copyright © 2017 Académie des sciences. Published by Elsevier Masson SAS. All rights reserved.

  13. Condições de armazenamento na viabilidade e dormência de sementes de videira Effect of storage conditions on the viability and dormancy of grape seeds

    Directory of Open Access Journals (Sweden)

    Jocely Andreuccetti Maeda

    1985-01-01

    Full Text Available A manutenção da viabilidade de sementes por períodos mais ou menos longos está bastante relacionada com as condições em que elas são armazenadas. Temperatura e teor de umidade estão entre os fatores que mais afetam sua longevidade. O objetivo deste trabalho é o estudo da viabilidade e dormência de uva do cultivar Patrícia, armazenada durante 24 meses em dois tipos de recipientes - em vidro hermético e saco de papel - a temperaturas de 10, 20 e 30°C, com dois diferentes teores iniciais de umidade. A porcentagem de sementes dormentes e viáveis foi determinada bimestralmente durante os primeiros 12 meses, e semestralmente nos períodos mais longos. O armazenamento não quebrou a dormência das sementes de uva em nenhuma das condições estudadas. Enquanto as sementes frescas mostraram melhor conservação a 10°C quando embaladas em vidro hermético, sementes secas se conservaram melhor a 20°C. Em saco de papel, foram poucas as diferenças encontradas entre os tratamentos, a não ser nos períodos finais do armazenamento, quando 20°C se mostrou a pior temperatura de conservação. Dessa maneira, a dormência não foi alterada pelo armazenamento, nas condições estudadas, e a viabilidade foi bastante afetada tanto pelo tipo de embalagem como pela temperatura de armazenamento.Longevity of seeds is affected by the storage conditions, being temperature and seed moisture content the major factors to keep seeds viable. Experiments described here are concerned to the study of dormancy ant viability of seeds of grape cv. Patrícia, stored with two different moisture contents, either in moisture proof containers or paper bags, at temperaturss of 10°C, 20°C and 30°C, for 24 months. Samples were taken every two months during the first 12 months and at 6 months intervals in the second year, to determine seed viability and dormancy level. Storage, in the studied conditions, did not alter dormancy levei of the seeds. When stored in hermetical

  14. PEG-lipid micelles enable cholesterol efflux in Niemann-Pick Type C1 disease-based lysosomal storage disorder

    Science.gov (United States)

    Brown, Anna; Patel, Siddharth; Ward, Carl; Lorenz, Anna; Ortiz, Mauren; DuRoss, Allison; Wieghardt, Fabian; Esch, Amanda; Otten, Elsje G.; Heiser, Laura M.; Korolchuk, Viktor I.; Sun, Conroy; Sarkar, Sovan; Sahay, Gaurav

    2016-01-01

    2-Hydroxy-propyl-β-cyclodextrin (HPβCD), a cholesterol scavenger, is currently undergoing Phase 2b/3 clinical trial for treatment of Niemann Pick Type C-1 (NPC1), a fatal neurodegenerative disorder that stems from abnormal cholesterol accumulation in the endo/lysosomes. Unfortunately, the extremely high doses of HPβCD required to prevent progressive neurodegeneration exacerbates ototoxicity, pulmonary toxicity and autophagy-based cellular defects. We present unexpected evidence that a poly (ethylene glycol) (PEG)-lipid conjugate enables cholesterol clearance from endo/lysosomes of Npc1 mutant (Npc1−/−) cells. Herein, we show that distearyl-phosphatidylethanolamine-PEG (DSPE-PEG), which forms 12-nm micelles above the critical micelle concentration, accumulates heavily inside cholesterol-rich late endosomes in Npc1−/− cells. This potentially results in cholesterol solubilization and leakage from lysosomes. High-throughput screening revealed that DSPE-PEG, in combination with HPβCD, acts synergistically to efflux cholesterol without significantly aggravating autophagy defects. These well-known excipients can be used as admixtures to treat NPC1 disorder. Increasing PEG chain lengths from 350 Da-30 kDa in DSPE-PEG micelles, or increasing DSPE-PEG content in an array of liposomes packaged with HPβCD, improved cholesterol egress, while Pluronic block copolymers capable of micelle formation showed slight effects at high concentrations. We postulate that PEG-lipid based nanocarriers can serve as bioactive drug delivery systems for effective treatment of lysosomal storage disorders. PMID:27572704

  15. The Peanut (Arachis hypogaea L. Gene AhLPAT2 Increases the Lipid Content of Transgenic Arabidopsis Seeds.

    Directory of Open Access Journals (Sweden)

    Silong Chen

    Full Text Available Lysophosphatidic acid acyltransferase (LPAT, which converts lysophosphatidic acid (LPA to phosphatidic acid (PA, catalyzes the addition of fatty acyl moieties to the sn-2 position of the LPA glycerol backbone in triacylglycerol (TAG biosynthesis. We recently reported the cloning and temporal-spatial expression of a peanut (Arachis hypogaea AhLPAT2gene, showing that an increase in AhLPAT2 transcript levels was closely correlated with an increase in seed oil levels. However, the function of the enzyme encoded by the AhLPAT2 gene remains unclear. Here, we report that AhLPAT2 transcript levels were consistently higher in the seeds of a high-oil cultivar than in those of a low-oil cultivar across different seed developmental stages. Seed-specific overexpression of AhLPAT2 in Arabidopsis results in a higher percentage of oil in the seeds and greater-than-average seed weight in the transgenic plants compared with the wild-type plants, leading to a significant increase in total oil yield per plant. The total fatty acid (FA content and the proportion of unsaturated FAs also increased. In the developing siliques of AhLPAT2-overexpressing plants, the expression levels of genes encoding crucial enzymes involved in de novo FA synthesis, acetyl-CoA subunit (AtBCCP2 and acyl carrier protein 1 (AtACP1 were elevated. AhLPAT2 overexpression also promoted the expression of several key genes related to TAG assembly, sucrose metabolism, and glycolysis. These results demonstrate that the expression of AhLPAT2 plays an important role in glycerolipid production in peanuts.

  16. The Effect of Gamma radiation, microwave radiation, their interaction and storage on chemical composition, antinutritional factors and the activities of trypsin inhibitor and lipoxygenase of soybean seeds

    Energy Technology Data Exchange (ETDEWEB)

    Abdel-Rahim, E A; Abdel-Fatah, O M [Dept. of Biochem., Faculty of Agric., Cairo University. (Egypt); El-Adawy, M; Badea, M Y [Food Technol. Dept., National Center for Research and Radiation Technol., Atomic Energy Authority (Egypt)

    2000-07-01

    The effect of gamma radiation, microwave radiation, interaction between them; and storage of radiated soybean seeds were investigated to find out the best treatment which had to the maximum reduction of antinutrional factors (Trypsin inhibitor and lipoxygenase activities) without significant effect on the chemical constituents. The gamma rays was used at three doses of 2.5, 5.0 and 8.0 kGy, microwave radiation was at 70 level power for 2 and 4 min; and the storage of seeds was at temperature, R.H. 50-55% for six months. The data revealed that, effects of interaction treatments were more effective than the treatment with microwave or gamma radiation alone.

  17. The Effect of Gamma radiation, microwave radiation, their interaction and storage on chemical composition, antinutritional factors and the activities of trypsin inhibitor and lipoxygenase of soybean seeds

    International Nuclear Information System (INIS)

    Abdel-Rahim, E.A.; Abdel-Fatah, O.M.; El-Adawy, M.; Badea, M.Y.

    2000-01-01

    The effect of gamma radiation, microwave radiation, interaction between them; and storage of radiated soybean seeds were investigated to find out the best treatment which had to the maximum reduction of antinutrional factors (Trypsin inhibitor and lipoxygenase activities) without significant effect on the chemical constituents. The gamma rays was used at three doses of 2.5, 5.0 and 8.0 kGy, microwave radiation was at 70 level power for 2 and 4 min; and the storage of seeds was at temperature, R.H. 50-55% for six months. The data revealed that, effects of interaction treatments were more effective than the treatment with microwave or gamma radiation alone

  18. The effect of melanin-free extract from Sepia esculenta ink on lipid peroxidation, protein oxidation and water-holding capacity of tilapia fillet during cold storage.

    Science.gov (United States)

    Duan, Zhen-Hua; Liu, Hua-Zhong; Luo, Ping; Gu, Yi-Peng; Li, Yan-Qun

    2018-03-14

    Preservative effect of melanin-free extract of Sepia esculenta ink (MFESI) on Sparus latus fillet has been verified in our previous work. This study aims to further approach the mechanism of MFESI for extending the shelf-life of fish fillet during cold storage. Tilapia fillets were treated with different dosage of MFESI (0, 15, 25 and 35 mg/ml) and packed with preservative film for succedent cold-storage at 4 °C for scheduled time. Contents of total volatile basic nitrogen and sulfydryl and carbanyl groups were measured for evaluating protein oxidation. Malondialdehyde contents were measured for estimating lipid peroxidation and loss of water was used to determine water-holding capacity of fillet. The data indicated that MFESI not only possessed certain degree of antioxidant capacity in vitro, also lengthened shelf-life of tilapia fillet in cold-storage condition. Apart from 15 mg/ml, both 25 and 35 mg/ml of MFESI obviously prevented lipid and protein from oxidation and reduced loss of water from tilapia fillets, and the latter was more effective than the former. MFESI can repress lipid peroxidation and protein oxidation and reduce water loss, maintain the tilapia fillets quality and, thus, it could be an effective and natural preservative for extending the shelf-life of tilapia fillets during cold storage.

  19. A study of biodiversity using DSS method and seed storage protein comparison of populations in two species of Achillea L. in the west of Iran

    Directory of Open Access Journals (Sweden)

    Hajar Salehi

    2013-11-01

    Full Text Available Intarspecific and interspecific variations are the main reserves of biodiversity and both are important sources of speciation. On this basis, identifing and recognizing the intra and interspecific variations is important in order to recognition of biodiversity. This research was done to study biodiversity and electrophoresis comparison of seed storage proteins in the populations of the two species of the genus Achillea in Hamadan and Kurdistan provinces using of the method of determination of special station (DSS. For this purpose, 12 and 9 special stations were selected for the species A. tenuifolia and A. biebresteinii using the data published in the related flora. Seed storage proteins were extracted and then studied using electrophoresis techniques (SDS-PAGE. In survey of all special stations, 120 plant species were distinguished as associated species. The results of the floristic data for the both species determined six distinctive groups that indicated the existence of intraspecific diversity in this species. The result of analysis of ecological data and seed storage proteins for the two species was in accordance with the floristic data and showed six distinctive groups. The existence of the bands of no. 4, 5, 8, 12 and 13 in the special stations of A. tenuifolia and the bands of 14, 15 and 16 in the special stations of A. biebresteinii o separated the populations of the species in two quite different and distinctive groups.

  20. Protein aggregation in aqueous casein solution. Effect of irradiation, dose level, concentration, storage and additives (carbohydrate and lipid)

    Energy Technology Data Exchange (ETDEWEB)

    Yousri, R M

    1980-06-01

    From the vast amount of research efforts dealing with various aspects of radiation effects on foods and food components, it is apparent up to now that much remains to be studied in depth, much may have to be added or corrected about radiation-induced physico-chemical changes in foods. A great many reactions that take place when foodstuffs are subjected to ionizing radiation are still not fully understood. The better understanding of some of the radiation-induced changes in pure proteins as such or in mixture with other food constituents could yield much data which could be meaningfully extrapolated to intact foods and consequently could help to improve the assessment of the wholesomeness of irradiated foods. It was the purpose of our investigations to elucidate some of the changes in the chemical structure of a pure protein (casein), irradiated as such or with added carbohydrate and/or lipid. The effect of subsequent storage of the irradiated solutions has been also examined. The formation of protein aggregates was studied by gel filtration technique. The application of thin-layer gel filtration, its speed and adaptability to very small samples facilitated the measurements of the extent of aggregation which occurred in protein molecules after irradiation.

  1. The perspective effects of various seed coating substances on rice seed variety Khao Dawk Mali 105 storability II: the case study of chemical and biochemical properties.

    Science.gov (United States)

    Thobunluepop, P; Pan-in, W; Pawelzik, E; Vearasilp, S

    2009-04-01

    The aim of this study was to investigate the effects of seed coating substances; chemical fungicide (CA) and biological fungicide polymers [chitosan-lignosulphonate polymer (CL) and eugenol incorporated into chitosan-lignosulphonate polymer (E+CL)] on chemical and biochemical changes of rice seeds cv. KDML 105, which have been studied during storage for 12 months. CA significantly affected the rice seed chemical properties and the associated seed deterioration. After 12 months storage, protein content decreased accompanied by declined of lipid content, increased free fatty acids and activated lipoxygenase enzyme. In the case of biological fungicide coated seeds, the antioxidative scavenging enzymes were ascorbate peroxidase and superoxide dismutase and a high antioxidant activity protected them. Moreover, the sugar content was positive correlated with seed germination and vigor. The biological coated seeds were found to maintain high sugar contents inside the seeds, which resulted high seed storability significantly. In contrast, under fungicide stress (CA), those compounds were lost that directly affected seed vigor during storage.

  2. Characterisation, immunolocalisation and antifungal activity of a lipid transfer protein from chili pepper (Capsicum annuum) seeds with novel α-amylase inhibitory properties.

    Science.gov (United States)

    Diz, Mariângela S; Carvalho, Andre O; Ribeiro, Suzanna F F; Da Cunha, Maura; Beltramini, Leila; Rodrigues, Rosana; Nascimento, Viviane V; Machado, Olga L T; Gomes, Valdirene M

    2011-07-01

    Lipid transfer proteins (LTPs) were thus named because they facilitate the transfer of lipids between membranes in vitro. This study was triggered by the characterization of a 9-kDa LTP from Capsicum annuum seeds that we call Ca-LTP(1) . Ca-LTP(1) was repurified, and in the last chromatographic purification step, propanol was used as the solvent in place of acetonitrile to maintain the protein's biological activity. Bidimensional electrophoresis of the 9-kDa band, which corresponds to the purified Ca-LTP(1) , showed the presence of three isoforms with isoelectric points (pIs) of 6.0, 8.5 and 9.5. Circular dichroism (CD) analysis suggested a predominance of α-helices, as expected for the structure of an LTP family member. LTPs immunorelated to Ca-LTP(1) from C. annuum were also detected by western blotting in exudates released from C. annuum seeds and also in other Capsicum species. The tissue and subcellular localization of Ca-LTP(1) indicated that it was mainly localized within dense vesicles. In addition, isolated Ca-LTP(1) exhibited antifungal activity against Colletotrichum lindemunthianum, and especially against Candida tropicalis, causing several morphological changes to the cells including the formation of pseudohyphae. Ca-LTP(1) also caused the yeast plasma membrane to be permeable to the dye SYTOX green, as verified by fluorescence microscopy. We also found that Ca-LTP(1) is able to inhibit mammalian α-amylase activity in vitro. Copyright © Physiologia Plantarum 2011.

  3. Drying and storage of Eugenia involucrata DC. seeds Secagem e armazenamento de sementes de Eugenia involucrata DC.

    Directory of Open Access Journals (Sweden)

    Angela Maria Maluf

    2003-01-01

    Full Text Available The physiological quality of seeds of native species is important to produce healthy saplings and therefore guarantee the success of programs to recover disturbed vegetation. This reinforces the necessity for investigating the physiological quality of those seeds. To evaluate the effects of different drying rates on the germination, moisture content and storability of Eugenia involucrata diaspores, mature fruits collected at Mogi Guaçu, SP, Brazil had their epi- and mesocarps removed by washing and were dried at 30, 40 or 50ºC until their water content was reduced from 57% (fresh diaspores to 13% (final drying, totaling six drying levels. In a second experiment, diaspores had their moisture content reduced from 57% to 49%, at 30ºC, totaling six drying levels (0h, 1h, 2h, 3h, 4h and 5h, and were kept for 180 days in plastic bags under cold storage. The drying rate had no effect on tolerance to desiccation by E. involucrata diaspores; water contents lower than 51% decreased both germinability and storability. Diaspores can be stored for up to 180 days as long as their water content is reduced to 53% and they are kept inside plastic bags under cold storage.O uso de sementes de espécies nativas de alta qualidade é fundamental nos programas de recomposição vegetal, o que fortalece a necessidade de se investigar o potencial fisiológico das mesmas. Esta pesquisa objetivou avaliar os efeitos da velocidade de secagem dos diásporos de Eugenia involucrata sobre a sua germinação e vigor, bem como as relações entre teor de água e capacidade de armazenamento. Foram colhidos frutos maduros em pomar instalado em Mogi Guaçu, SP (22º15-16'S, 47º8-12'W, que tiveram seu epicarpo e mesocarpo removidos por lavagem. A seguir, os diásporos (semente + endocarpo foram submetidos a secagem controlada a 30, 40 e 50ºC, com reduções progressivas do teor de água inicial de 57% para até 13%, obtendo-se seis níveis de secagem em cada temperatura. Em um

  4. Effect of absorbed dose and storage length on electron paramagnetic resonance (EPR) signal strength in irradiated alfalfa seeds

    International Nuclear Information System (INIS)

    Li Naining

    2006-01-01

    A kind of alfalfa seeds was irradiated by 1, 2, 3, 4 and 5 kGy at a dose rate of 6.288 kGy·h -1 in a self-shielded irradiator of 137 Cs gamma rays. The EPR spectra, which were measured subsequently between 0.3401 and 0.3501 T, showed that there was a direct proportional relationship between the EPR signal strength of free radicals produced by gamma irradiation in the alfalfa seeds and absorbed dose. The first derivative EPR spectra of the alfalfa seeds were very clear and easy to identify. However, the EPR signal strength of the peak-to-peak amplitude decreased rapidly and most of them decayed beyond 50% within 3 days after the seeds were irradiated. It tended to stabilize after half a month since the seeds were irradiated. the differences of the EPR signal strength between the irradiated and unirradiated alfalfa seeds still remained. All seeds were stored at ambient temperature for more than 3 months. Therefore, using EPR spectrometry technique to measure free radicals in alfalfa seeds as a means to determine whether the seeds have been irradiated or not is feasible, relatively fast and simple. (authors)

  5. Exercise Training and Grape Seed Extract Co-Administration Improves Lipid Profile, Weight Loss, Bradycardia, and Hypotension of STZ-Induced Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Mohammad Badavi

    2013-12-01

    Full Text Available Background:: Exercise Training (ET and Grape Seed Extract (GSE as an antioxidant have many positive effects on controlling diabetes mellitus and its complications. Objectives:: This study aimed to determine the effects of GSE alone or combined with ET on body weight, plasma lipid profile, blood pressure, and heart rate in STZ-induced diabetic rats. Methods:: In this study, male Wistar rats were randomly assigned to five groups: sedentary control, sedentary diabetic, trained diabetic, GSE treated sedentary diabetic, and GSE treated trained diabetic. ET was conducted on the treadmill daily for 8 weeks. One way ANOVA followed by LSD test was used for statistical analysis. Results:: Reduction of body weight, high density lipoproteins, heart rate, and systolic blood pressure and increment of total cholesterol, triglyceride, low density lipoprotein, and very low density lipoproteins were observed after STZ injection. Co-administration of GSE and ET had more positive effects on lipid profile compared to each method alone. In addition, GSE and ET modified heart rate partially, while their combination was more effective in improvement of heart rat in conscious rats. On the other hand, administration of ET or GSE alone did not affect systolic blood pressure and body weight, while their combination restored systolic blood pressure completely and improved body weight partially. Conclusions:: The study findings indicated that ET combined with GSE had more beneficial effects compared to each one alone on the complications of STZ induced diabetes. This may constitute a convenient and inexpensive therapeutic approach to diabetic complications.

  6. Global analysis of gene expression profiles in developing physic nut (Jatropha curcas L.) seeds.

    Science.gov (United States)

    Jiang, Huawu; Wu, Pingzhi; Zhang, Sheng; Song, Chi; Chen, Yaping; Li, Meiru; Jia, Yongxia; Fang, Xiaohua; Chen, Fan; Wu, Guojiang

    2012-01-01

    Physic nut (Jatropha curcas L.) is an oilseed plant species with high potential utility as a biofuel. Furthermore, following recent sequencing of its genome and the availability of expressed sequence tag (EST) libraries, it is a valuable model plant for studying carbon assimilation in endosperms of oilseed plants. There have been several transcriptomic analyses of developing physic nut seeds using ESTs, but they have provided limited information on the accumulation of stored resources in the seeds. We applied next-generation Illumina sequencing technology to analyze global gene expression profiles of developing physic nut seeds 14, 19, 25, 29, 35, 41, and 45 days after pollination (DAP). The acquired profiles reveal the key genes, and their expression timeframes, involved in major metabolic processes including: carbon flow, starch metabolism, and synthesis of storage lipids and proteins in the developing seeds. The main period of storage reserves synthesis in the seeds appears to be 29-41 DAP, and the fatty acid composition of the developing seeds is consistent with relative expression levels of different isoforms of acyl-ACP thioesterase and fatty acid desaturase genes. Several transcription factor genes whose expression coincides with storage reserve deposition correspond to those known to regulate the process in Arabidopsis. The results will facilitate searches for genes that influence de novo lipid synthesis, accumulation and their regulatory networks in developing physic nut seeds, and other oil seeds. Thus, they will be helpful in attempts to modify these plants for efficient biofuel production.

  7. Storage of sorghum seeds harvested with different moisture levels / Armazenamento de sementes de sorgo submetidas a diferentes graus de umidade de colheita

    Directory of Open Access Journals (Sweden)

    Adílio de Sá Júnior

    2009-12-01

    Full Text Available The aim of this research work was to study the effect of storage in a climatized environment on the physiologic quality of hybrid of sorghum seeds harvested with different moisture contents and submitted to storage, in Uberlândia-MG. The seeds used were collected in a commercial seed production field of Monsanto Brasil Ltd., in the cropping season 2002/2003. The samples were placed in multi-layer paper bags, stored in a climatized warehouse at the processing unit of Monsanto Brasil Ltd. The experimental design was randomized blocks with split plots on time. The experiment had seed samples harvested with four moisture contents (20%, 18%, 14% and 11% and four storage times (beginning of storage, 10, 22 and 28 months. The changes on seed physiologic quality were evaluated by germination test (normal or abnormal seedlings, cold test (vigour. Also, the moisture content of the seeds was analyzed during storage. From the results, it can be concluded that: a as harvest proceeded with greater moisture contents, the physiologic quality of these seeds decreased; b seed physiologic quality decreased significantly; however, the germination percentage remained within the commercialization standard until 28 months.Sementes híbridas de sorgo foram colhidas com diferentes graus de umidade e armazenadas em ambiente climatizado por 28 meses, em Uberlândia-MG. Utilizaram-se sementes obtidas de um experimento conduzido em campo de produção comercial da Monsanto do Brasil Ltda., safra 2002/2003. As amostras das parcelas foram acondicionadas em sacos de papel multifoliados e armazenadas em armazém climatizado (temperatura de 10ºC e umidade relativa de 50% na unidade de beneficiamento da Monsanto do Brasil Ltda. O delineamento experimental foi o de blocos casualizados com parcelas subdivididas no tempo. Na parcela os tratamentos constituíram-se por amostras de sementes colhidas com quatro graus de umidade (20%, 18%, 14% e 11%, e nas subparcelas avaliou

  8. Neuronal and epithelial cell rescue resolves chronic systemic inflammation in the lipid storage disorder Niemann-Pick C.

    Science.gov (United States)

    Lopez, Manuel E; Klein, Andrés D; Hong, Jennifer; Dimbil, Ubah J; Scott, Matthew P

    2012-07-01

    Chronic systemic inflammation is thought to be a major contributor to metabolic and neurodegenerative diseases. Since inflammatory components are shared among different disorders, targeting inflammation is an attractive option for mitigating disease. To test the significance of inflammation in the lipid storage disorder (LSD) Niemann-Pick C (NPC), we deleted the macrophage inflammatory gene Mip1a/Ccl3 from NPC diseased mice. Deletion of Ccl3 had been reported to delay neuronal loss in Sandhoff LSD mice by inhibiting macrophage infiltration. For NPC mice, in contrast, deleting Ccl3 did not retard neurodegeneration and worsened the clinical outcome. Depletion of visceral tissue macrophages also did not alter central nervous system (CNS) pathology and instead increased liver injury, suggesting a limited macrophage infiltration response into the CNS and a beneficial role of macrophage activity in visceral tissue. Prevention of neuron loss or liver injury, even at late stages in the disease, was achieved through specific rescue of NPC disease in neurons or in liver epithelial cells, respectively. Local epithelial cell correction was also sufficient to reduce the macrophage-associated pathology in lung tissue. These results demonstrate that elevated inflammation and macrophage activity does not necessarily contribute to neurodegeneration and tissue injury, and LSD defects in immune cells may not preclude an appropriate inflammatory response. We conclude that inflammation remains secondary to neuronal and epithelial cell dysfunction and does not irreversibly contribute to the pathogenic cascade in NPC disease. Without further exploration of possible beneficial roles of inflammatory mediators, targeting inflammation may not be therapeutically effective at ameliorating disease severity.

  9. LipidPedia: a comprehensive lipid knowledgebase.

    Science.gov (United States)

    Kuo, Tien-Chueh; Tseng, Yufeng Jane

    2018-04-10

    Lipids are divided into fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, saccharolipids, sterols, prenol lipids and polyketides. Fatty acyls and glycerolipids are commonly used as energy storage, whereas glycerophospholipids, sphingolipids, sterols and saccharolipids are common used as components of cell membranes. Lipids in fatty acyls, glycerophospholipids, sphingolipids and sterols classes play important roles in signaling. Although more than 36 million lipids can be identified or computationally generated, no single lipid database provides comprehensive information on lipids. Furthermore, the complex systematic or common names of lipids make the discovery of related information challenging. Here, we present LipidPedia, a comprehensive lipid knowledgebase. The content of this database is derived from integrating annotation data with full-text mining of 3,923 lipids and more than 400,000 annotations of associated diseases, pathways, functions, and locations that are essential for interpreting lipid functions and mechanisms from over 1,400,000 scientific publications. Each lipid in LipidPedia also has its own entry containing a text summary curated from the most frequently cited diseases, pathways, genes, locations, functions, lipids and experimental models in the biomedical literature. LipidPedia aims to provide an overall synopsis of lipids to summarize lipid annotations and provide a detailed listing of references for understanding complex lipid functions and mechanisms. LipidPedia is available at http://lipidpedia.cmdm.tw. yjtseng@csie.ntu.edu.tw. Supplementary data are available at Bioinformatics online.

  10. Fatty acid profile, color and lipid oxidation of organic fermented sausage during chilling storage as influenced by acid whey and probiotic strains addition

    Directory of Open Access Journals (Sweden)

    Karolina Maria Wójciak

    2015-02-01

    Full Text Available Organic fermented sausages typically spoil during long-term storage due to oxidative rancidity. The application of natural antioxidants to meat stuffing is a major practice intended to inhibit the oxidation process and color changes. This study aimed to assess the effect of two unusual starter cultures: three probiotic strains (Lactobacillus casei LOCK 0900, Lactobacillus casei LOCK 0908 and Lactobacillus paracasei LOCK 0919 and lactic acid bacteria from acid whey on model fermented sausage type products focusing on oxidative stability by measuring instrumental color (L*, a*, b* values, conjugated dienes (CD, TBARS immediately after 21 days of ripening (0 and after 90 and 180 days of refrigerated storage (4 ºC. Determination of fatty acid composition, in meat product was performed after ripening and after 180 days of storage. At the end of the storage period, the salted sausages were characterized by the same content of polyunsaturated fatty acids (PUFA compared to cured samples. The addition of acid whey and a mixture of probiotic strains to nitrite-free sausage formulation was barely able to protect lipids against oxidation in comparison to nitrite during vacuum storage. Surprisingly, the use of acid whey has an influence on the desired red-pinkish color of organic fermented sausage after ripening and after 180 days of storage period.

  11. Effect of Long Period Cooling Storage on the Nucleic Acid of Harvested Cowpea Seeds ( Vigna Sinensis L.) Treated by Gamma Irradiation and Micro Elements

    International Nuclear Information System (INIS)

    Saleh, O.I.; Salama, I.M.

    2015-01-01

    Cowpea seeds ( Vigna sinensis L.) were exposed to 40 and 80 Gy gamma radiation, in order to study the effect of long period under cooling storage by using RAPD and ISSR PCR facilities. The obtained results indicated that RAPD protocol gave 65% monomorphic and 56% polymorphic fragments between the samples as compared to storage and non-storage controls. While , ISSR protocol gave 83% monomorphic and 85% polymorphic fragments. It should be mentioned that other percentage s 86% and 91% were found among samples in case of using another primers. The results could be summarized as follow: 1-Primer OP - B01 gave 7 monomorphic and 13 polymorphic fragments (65%). 2 - The Primer OP - B02 and Primer OP - B05 gave 4 monomorphic fragments with 14 polymorphic fragments (79%). 3 - The Primer HA-98 gave 4 monomorphic fragments with 19 detected polymorphic 83%. 4 - The Primer HA - 99 and HB-12 gave 3 monomorphic fragments and 17 polymorphic 85 and 86%, respectively. 5 - The Primer HB - 13 gave 2 monomorphic fragments with 21 detected polymorphic fragments 91%. 6 - The primer HA-98 gave 83% while the primer HA - 99 gave 85%. The previous results showed some polymorphism differences among the samples, while the primer HB-12 gave 86% and the primer HB-13 91% exhibited high levels of polymorphism. The DNA of stored cowpea seeds which were exposed to 80 Gy in the presence of zinc showed the highest differentiation , while radiation dose 40 Gy treated with zinc or boron, 80 Gy with boron and 40 or 80 Gy treatment alone compared to the two controls (storage and non storage)

  12. Effect of an aqueous extract of annatto (Bixa orellana seeds on lipid profile and biochemical markers of renal and hepatic function in hipercholesterolemic rats

    Directory of Open Access Journals (Sweden)

    Heberth de Paula

    2009-12-01

    Full Text Available Annatto extract is a natural food color obtained from the outer coatings of the seeds of the Annatto tree (Bixa orellana L.. This is the first report in the literature that shows the relationship between the aqueous annatto extract and its influence on lipid profile in animals. Male Fisher rats were divided into three groups (n=12: C group, fed standard diet and water; H group, fed high-lipid diet and water and; HU group, with high-lipid diet and aqueous annatto extract for 60 days. The treatment with annatto extract in animals fed with the high-lipid diet lowered the LDL- and total cholesterol and raised the HDL-cholesterol, suggesting a hypocholesterolemic effect. Neither high-fat diet nor aqueous annatto extract had any significant effect on serum levels of albumin or serum activities of transaminases which suggested that no liver injury was induced.Extrato de urucum é um corante alimentar natural que é obtido da casca das sementes do urucueiro (Bixa orellana L.. Este é o primeiro trabalho da literatura que mostra a relação entre o extrato aquoso de urucum e sua influência no perfil lipídico de animais. Durante 60 dias, 36 ratos machos Fisher foram divididos em 3 grupos: Grupo C que recebeu uma dieta controle e água; Grupo H que recebeu uma dieta rica em lipídios e água e; Grupo HU que recebeu uma dieta rica em lipídios e extrato aquoso de semente de urucum. O tratamento com extrato de urucum nos animais alimentados com a dieta rica em lipídios abaixou o colesterol total e a fração LDL e aumentou a fração HDL, sugerindo um efeito hipocolesterolemiante. Nem a dieta rica em lipídios, nem o extrato de urucum tiveram algum efeito sobre os níveis séricos de albumina ou sobre a atividade de alanina aminotransferase e aspartato aminotransferase. Este fato sugere que o consumo do extrato não provocou injúria hepática nos animais.

  13. Oxidation of lipid and protein in horse mackerel (Trachurus trachurus) mince and washed minces during processing and storage

    DEFF Research Database (Denmark)

    Eymard, Sylvie; Baron, Caroline; Jacobsen, Charlotte

    2009-01-01

    : M1, M2 and M3, with one, two and three washing steps, respectively. The different products were characterised (i.e. lipid content, protein, water, iron, fatty acid profile and tocopherol content) and analysed for protein and lipid oxidation in order to investigate the impact of the washing steps...... was followed by determination of protein solubility, protein thiol groups and protein carbonyl groups using colorimetric methods as well as western blotting for protein carbonyl groups. Lipid and protein oxidation markers indicated that both lipid and protein oxidation took place during processing...

  14. Lipid Storage Diseases

    Science.gov (United States)

    ... Institutes of Health (NIH), the leading supporter of biomedical research in the world. As part of its mission, ... develop treatments, which has enabled ongoing and promising research to develop gene therapy for this disease. The NINDS, along with other ...

  15. Assessment of genetic fidelity in Rauvolfia serpentina plantlets grown from synthetic (encapsulated) seeds following in vitro storage at 4 °C.

    Science.gov (United States)

    Faisal, Mohammad; Alatar, Abdulrahman A; Ahmad, Naseem; Anis, Mohammad; Hegazy, Ahmad K

    2012-05-03

    An efficient method was developed for plant regeneration and establishment from alginate encapsulated synthetic seeds of Rauvolfia serpentina. Synthetic seeds were produced using in vitro proliferated microshoots upon complexation of 3% sodium alginate prepared in Llyod and McCown woody plant medium (WPM) and 100 mM calcium chloride. Re-growth ability of encapsulated nodal segments was evaluated after storage at 4 °C for 0, 1, 2, 4, 6 and 8 weeks and compared with non-encapsulated buds. Effects of different media viz; Murashige and Skoog medium; Lloyd and McCown woody Plant medium, Gamborg’s B5 medium and Schenk and Hildebrandt medium was also investigated for conversion into plantlets. The maximum frequency of conversion into plantlets from encapsulated nodal segments stored at 4 °C for 4 weeks was achieved on woody plant medium supplement with 5.0 μM BA and 1.0 μM NAA. Rooting in plantlets was achieved in half-strength Murashige and Skoog liquid medium containing 0.5 μM indole-3-acetic acid (IAA) on filter paper bridges. Plantlets obtained from stored synseeds were hardened, established successfully ex vitro and were morphologically similar to each other as well as their mother plant. The genetic fidelity of Rauvolfia clones raised from synthetic seeds following four weeks of storage at 4 °C were assessed by using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. All the RAPD and ISSR profiles from generated plantlets were monomorphic and comparable to the mother plant, which confirms the genetic stability among the clones. This synseed protocol could be useful for establishing a particular system for conservation, short-term storage and production of genetically identical and stable plants before it is released for commercial purposes.

  16. Armazenamento de sementes de braquiária peletizadas e tratadas com fungicida e inseticida Brachiaria coated seed storage treated with fungicide and insecticide

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Pereira

    2011-12-01

    Full Text Available A qualidade de sementes é fundamental para o sucesso da formação de pastagem, de forma que é importante viabilizar tecnologias para elas. Assim, objetivou-se com este trabalho avaliar o desempenho de sementes de Brachiaria decumbens peletizadas e tratadas com fungicida e inseticida, durante o armazenamento. As sementes foram tratadas com thiabendazol na dosagem de 200mL 100kg-1 de sementes, com fipronil na dosagem de 500mL 100kg-1 de sementes e com a mistura de ambos (nas mesmas dosagens e parte não foi submetida a esses tratamentos (testemunha. Posteriormente, as sementes foram peletizadas utilizando-se uma mistura de areia + microcelulose e cola Cascorex - PVA (20%, as quais em seguida foram armazenadas em condições ambientais em armazém convencional (temperatura e umidade relativa do ar média de 21,9°C e 68%, respectivamente, durante 12 meses. As sementes foram avaliadas inicialmente e a cada quatro meses com as seguintes avaliações: teor de água, teste de germinação, índice de velocidade de germinação, teste de emergência e índice de velocidade de emergência. A peletização das sementes de Brachiaria decumbens com areia e microcelulose prejudica a porcentagem e velocidade de germinação, bem como a emergência de plântulas durante o armazenamento. Sementes de Brachiaria decumbens cv. 'Basilisk' tratadas com fipronil, thiabendazol, ou com ambos, não devem ser armazenadas por mais de oito meses.The quality of seed is crucial to the success of pasture formation. Thus the aim of this research was to evaluate the performance of Brachiaria decumbens seeds pelleted and treated with fungicides and insecticides during storage. The seeds were treated with thiabendazole at a dosage of 200mL 100kg-1 of seeds, with fipronil at a dosage of 500mL 100kg-1 of seeds and a mixture of both (in the same dosages and some was not subjected to such treatment (control. Subsequently the seeds were coated using a mixture of sand

  17. Tratamento fungicida e peliculização de sementes de soja submetidas ao armazenamento Fungicide treatment and film coating of soybean seeds submitted to storage

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Pereira

    2011-02-01

    Full Text Available O tratamento de sementes de soja com fungicidas vem sendo utilizado como importante ferramenta no controle de patógenos. Nesse trabalho, objetivou-se estudar o desempenho de sementes de soja tratadas com fungicidas e peliculizadas, antes e após o armazenamento. Foram utilizados cinco lotes de sementes, cultivar Monsoy 6101, submetidos aos tratamentos: thiabendazole+thiram sem polímero, carbendazin+thiram sem polímero, sem fungicida (testemunha sem polímero, além desses tratamentos realizados via peliculização (com polímero. A qualidade fisiológica e sanitária das sementes foi avaliada inicialmente e após seis meses de armazenamento em condições ambientais, pelas seguintes determinações: teor de água, teste de germinação, emergência em bandeja, teste de frio e de sanidade. Conclui-se que a peliculização, em associação com fungicidas, não afeta a qualidade fisiológica das sementes de soja e o tratamento de lotes de sementes de soja com os fungicidas thiabendazole+thiram e carbendazin+thiram melhora seu desempenho e qualidade sanitária.The treatment of soybean seeds by fungicides has been used as an important tool in pathogen control. The objective of this work was to verify the performance of soybean seeds treated with fungicide and film coating, prior and after storage. Five seed lots, cultivar Monsoy 6101, were treated with thiabendazole+thiram without polymer, carbendazin+thiram without polymer, without fungicide (control, without polymer, and treatments by film coating (with polymer. The physiological and sanitary quality of the seeds was evaluated initially and after six months storage, under environmental conditions, according to the following characteristics: water content, test of germination and emergence on tray, blotter test and cold test. The film coating associated with fungicides does not affect the physiological quality of soybean seeds and the soybean seed treatment with the fungicides thiabendazole

  18. Effects of gamma irradiation on long-storage seeds of Oryza sativa (cv. 2233) and their surface infecting fungal diversity

    International Nuclear Information System (INIS)

    Maity, J.P.; Kar, S.; Banerjee, S.; Chakraborty, A.; Santra, S.C.

    2009-01-01

    We have reported an effective 60 Co gamma irradiation method for the removal and long-time prevention of contaminating fungi of Oryza sativa cv-2233, without the losses of seed viabilities. The fungal growth and their population on gamma treated seeds was found to decrease significantly (p 50%) at 2 kGy, whereas total inhibition was noticed at 3 kGy after 1.5 month.

  19. Endophytic bacterial effects on seed germination and mobilization of reserves in ammodendron biofolium

    International Nuclear Information System (INIS)

    Zhu, Y.; She, X.P.

    2017-01-01

    The main aim of this study was to analyze the mobilization of storage reserves during seed germination of Ammodendron bifolium by host plant-endophytic bacteria interaction and to determine the contribution of endophytic bacteria in plant establishment. The seeds were inoculated with three different endophytic bacteria from A. bifolium, Staphylococcus sp. AY3, Kocuria sp. AY9 and Bacillus sp. AG18, and they were germinated in the dark. Fresh weight changes and early seedling growth were assessed, and the content of storage compounds was quantified using biochemical assays in all germinated and non-germinated seeds. To understand the mechanism promoting seed germination, the activities of extracellular enzymes of bacterial isolates were also analyzed by the plate assay method. The results showed that treatment with endophytic bacteria accelerated seed germination; promoted further water absorption and radicle growth; and also promoted degradation of sucrose, protein and lipids during the germination process. At the same time, our results also showed that strain AG18 was able to produce protease and amylase, strain AY9 had only amylase activity, and strain AY3 had no extracellular enzyme activity. In summary, our current study showed that (i) endophytic bacteria improved seed germination and post-germination seedling growth of A. bifolium; (ii) inoculation with endophytic bacteria could promote storage reserve mobilization during or following germination; (iii) the degradation of protein, lipids and sucrose could provide essential energy for post-germination growth; and (iv) three bacterial isolates might have different action mechanisms on seed germination. (author)

  20. Comparison of the Effects of Edible Oils: Rice Bran, Grape Seed, and Canola on Serum Lipid Profile and Paraoxonase Activity in Hyperlipidemic Rats

    Directory of Open Access Journals (Sweden)

    Maryam Ranjbar-Zahedani

    2015-03-01

    Full Text Available Background: Dyslipidemia is considered as one of the crucial contributors to cardio- cerebro-vascular diseases. Objectives: The present study aimed to compare the effects of Rice Barn Oil (RBO, Grape Seed Oil (GSO, and Canola Oil (CO on dyslipidemia and oxidative stress in experimentally induced hyperlipidemic rats. Materials and Methods: In the present experimental study, forty hyperlipidemic male Wistar rats were randomly assigned to 4 groups to receive RBO, GSO, or CO or Soy Bean Oil (SBO, as controls, for 4 weeks following a 3-week period of Atherogenic Diet (AD intake. Blood samples were collected at the beginning of the study, after inducing dyslipidemia, and at the end of the experimental period. Then, the data were entered into the SPSS statistical software (v. 13.0 and analyzed using paired t-test, paired sample Wilcoxon signed rank test, and Kruskal-Wallis test. Results: AD elevated lipid and/or lipoprotein profile and decreased the paraoxonase activity in the hyperlipidemic rats. The results of paired t-test revealed that RBO led to a significant improvement in serum lipoprotein profile and paraoxonase activity. Besides, a significant difference was found in the GSO group regarding all the measured parameters, except for paraoxonase activity. Moreover, CO diet showed a significant hypolipidemic effect on serum Triglyceride (TG and Total Cholesterol (TC and led to a slight improvement in Low Density Lipoprotein-Cholesterol (LDL-C and High Density Lipoprotein-Cholesterol (HDL-C. Conclusions: The results of the present study suggested that vegetable oils, including RBO, GSO, and CO, might improve dyslipidemia and oxidative stress in hyperlipidemic rats. Indeed, substituting saturated fatty acids with unsaturated fatty acids in rats’ diet had beneficial effects on serum lipid profile and oxidative stress. Comparison of the 3 edible oils showed that GSO had a more profound effect on decreasing hyperlipidemia.

  1. Coordination of storage lipid synthesis and membrane biogenesis: evidence for cross-talk between triacylglycerol metabolism and phosphatidylinositol synthesis.

    Science.gov (United States)

    Gaspar, Maria L; Hofbauer, Harald F; Kohlwein, Sepp D; Henry, Susan A

    2011-01-21

    Despite the importance of triacylglycerols (TAG) and steryl esters (SE) in phospholipid synthesis in cells transitioning from stationary-phase into active growth, there is no direct evidence for their requirement in synthesis of phosphatidylinositol (PI) or other membrane phospholipids in logarithmically growing yeast cells. We report that the dga1Δlro1Δare1Δare2Δ strain, which lacks the ability to synthesize both TAG and SE, is not able to sustain normal growth in the absence of inositol (Ino(-) phenotype) at 37 °C especially when choline is present. Unlike many other strains exhibiting an Ino(-) phenotype, the dga1Δlro1Δare1Δare2Δ strain does not display a defect in INO1 expression. However, the mutant exhibits slow recovery of PI content compared with wild type cells upon reintroduction of inositol into logarithmically growing cultures. The tgl3Δtgl4Δtgl5Δ strain, which is able to synthesize TAG but unable to mobilize it, also exhibits attenuated PI formation under these conditions. However, unlike dga1Δlro1Δare1Δare2Δ, the tgl3Δtgl4Δtgl5Δ strain does not display an Ino(-) phenotype, indicating that failure to mobilize TAG is not fully responsible for the growth defect of the dga1Δlro1Δare1Δare2Δ strain in the absence of inositol. Moreover, synthesis of phospholipids, especially PI, is dramatically reduced in the dga1Δlro1Δare1Δare2Δ strain even when it is grown continuously in the presence of inositol. The mutant also utilizes a greater proportion of newly synthesized PI than wild type for the synthesis of inositol-containing sphingolipids, especially in the absence of inositol. Thus, we conclude that storage lipid synthesis actively influences membrane phospholipid metabolism in logarithmically growing cells.

  2. In vivo hepatic lipid quantification using MRS at 7 Tesla in a mouse model of glycogen storage disease type 1a.

    Science.gov (United States)

    Ramamonjisoa, Nirilanto; Ratiney, Helene; Mutel, Elodie; Guillou, Herve; Mithieux, Gilles; Pilleul, Frank; Rajas, Fabienne; Beuf, Olivier; Cavassila, Sophie

    2013-07-01

    The assessment of liver lipid content and composition is needed in preclinical research to investigate steatosis and steatosis-related disorders. The purpose of this study was to quantify in vivo hepatic fatty acid content and composition using a method based on short echo time proton magnetic resonance spectroscopy (MRS) at 7 Tesla. A mouse model of glycogen storage disease type 1a with inducible liver-specific deletion of the glucose-6-phosphatase gene (L-G6pc(-/-)) mice and control mice were fed a standard diet or a high-fat/high-sucrose (HF/HS) diet for 9 months. In control mice, hepatic lipid content was found significantly higher with the HF/HS diet than with the standard diet. As expected, hepatic lipid content was already elevated in L-G6pc(-/-) mice fed a standard diet compared with control mice. L-G6pc(-/-) mice rapidly developed steatosis which was not modified by the HF/HS diet. On the standard diet, estimated amplitudes from olefinic protons were found significantly higher in L-G6pc(-/-) mice compared with that in control mice. L-G6pc(-/-) mice showed no noticeable polyunsaturation from diallylic protons. Total unsaturated fatty acid indexes measured by gas chromatography were in agreement with MRS measurements. These results showed the great potential of high magnetic field MRS to follow the diet impact and lipid alterations in mouse liver.

  3. Effect of Grape Seed Extract on Lipid Profile and Expression of Interleukin-6 in Polycystic Ovarian Syndrome Wistar Rat Model

    Directory of Open Access Journals (Sweden)

    Zohreh Salmabadi

    2017-09-01

    Full Text Available Background Polycystic ovary syndrome (PCOS is a common but complex endocrine disorder and is the major cause of anovulation and consequent subfertility. In this study the effect of grape seed extract (GSE on triglyceride (TG, total cholesterol (TC, highdensity lipoprotein-cholestrol (HDL-C, low-density lipoprotein-cholestrol (LDL-C and interleukin-6 (IL-6 in PCOS Wistar rats were assessed. Materials and Methods In this experimental study, 84 adult female Wistar rats were divided into 7 groups (n=12 including control (intact, Sham (estradiol valerate solvent injection, control PCOS and 4 experimental PCOS groups. To induce the syndrome, a single subcutaneous injection of 2 mg estradiol valerate was applied. In experimental groups, PCOS rats were treated with different doses of 50, 75, 100 and 200 mg/kg body weight (BW GSE by intraperitoneal injection for 10 consecutive days. After harvesting blood serum, TG was measured by Glycerol-3-phosphate Oxidase-Peoxidase (GPO- PAP, TC by Cholesterol Oxidase-Peroxidase (CHOD-PAP, and HDL-C by sedimentation method, LDL-C by Friedwald calculation and IL-6 by ELISA method. The serum values of each parameter were analyzed using one-way ANOVA at P≤0.05. Results In all experimental groups significant decrease of visceral fat was obvious as compared with control PCOS group. LDL-C, TC and IL-6 levels in experimental groups, particularly at dose of 50 mg/kg of GSE, were significantly decreased as compared with PCOS group. However, HDL-C levels were not significantly changed. Conclusion : According to the findings of this study, it can be concluded that GSE with its effects on serum TC, LDL-C and IL-6 could reduce the effects of dyslipidemia and inflammation in PCOS rats and improve systemic symptoms of PCOS.

  4. Effect of direct seeding mulch-based systems on soil carbon storage and macrofauna in central Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Blanchart, E.; Bernoux, M.; Sarda, X.; Feller, C. [Institut de Recherche pour le Developpement, Montpellier (France); Siqueira Neto, M.; Cerri, C.C.; Piccolo, M. [CENA-USP, Piracicaba (Brazil). Lab. Biogeoquimica Ambiental; Douzet, J.M. [CIRAD, Antsirabe (Madagascar); Scopel, E. [CIRAD-CA, Planaltina (Brazil)

    2007-07-01

    Soils represent a large carbon pool, approximately 1500 Gt, equivalent to almost three times the quantity stored in terrestrial biomass and twice the amount stored in the atmosphere. The management and maintenance of soil carbon is therefore an integral part of the global carbon cycle. Land use change, inappropriate agricultural practices and climate change can all lead to a net release of C from soils to the atmosphere, exacerbating the problems of greenhouse gas release. Any modification of land-use or land management can induce variations in soil carbon stocks, even in agricultural systems that are perceived to be in a steady state. These modifications also alter soil macrofauna that is known to affect soil carbon dynamics. Direct seeding Mulch-based Cropping (DMC) systems with two crops per year without soil tillage have widely been adopted over the last 10 to 15 years in the Cerrado (central region) of Brazil. They are replacing the traditional soybean monocropping with fallow under conventional tillage (CT). Th e objective of this study was to examine how DMC practices affect soil organic carbon (SOC) dynamics and macrofauna (Rio Verde, Goias State). The approach was to determine soil C stocks and macrofauna in five fi elds under DMC aged 1, 5, 7, 11 and 13 years. In order to compare DMC systems with the native system of the region and previous land-use, a situation under native Cerrado (tree-savanna like vegetation) and a field conducted traditionally (CT) were also studied. Soil C stocks were calculated for the 0-10 and 0-40 cm soil depth and also for the fi rst 400 kg m{sup -2} of soil to compare the same amount of soil and to suppress the potential artefact of soil compaction when sample is based on fix layer depth. Soil macrofauna was hand-sorted from soil monoliths (30 cm depth, TSBF method). In our study, the annual rate of carbon storage was equal to ca. 1.6 MgC ha{sup -1}, which is in the range of values measured for DMC in different areas of Brazil

  5. Novel duplication mutation in the patatin domain of adipose triglyceride lipase (PNPLA2) in neutral lipid storage disease with severe myopathy.

    Science.gov (United States)

    Akiyama, Masashi; Sakai, Kaori; Ogawa, Masaya; McMillan, James R; Sawamura, Daisuke; Shimizu, Hiroshi

    2007-12-01

    Recently, mutations in PNPLA2 encoding adipose triglyceride lipase (ATGL) were reported to underlie a neutral lipid storage disease (NLSD) subgroup characterized by mild myopathy and the absence of ichthyosis. In the present study a novel homozygous PNPLA2 mutation c.475_478dupCTCC (p.Gln160ProfsX19) in the patatin domain, the ATGL active site, was detected in a woman with NLSD and severe myopathy. The present results suggest that a premature truncation mutation in the patatin domain causes NLSD with severe myopathy.

  6. Effects of Tannic Acid on Lipid and Protein Oxidation, Color, and Volatiles of Raw and Cooked Chicken Breast Meat during Storage

    Directory of Open Access Journals (Sweden)

    Marwan Al-Hijazeen

    2016-06-01

    Full Text Available The objective of this study was to determine the effect of tannic acid (TA on the oxidative stability and the quality characteristics of ground chicken breast meat. Five treatments including (1 control (none added, (2 2.5 ppm TA, (3 5 ppm TA, (4 10 ppm TA, and (5 5 ppm butylated hydroxyanisole (BHA were added to boneless, skinless ground chicken breast meat, and used for both raw and cooked meat studies. For the raw meat study, the ground chicken breast meat was packaged in oxygen-permeable bags and stored at 4 °C for 7 days. For the cooked study, raw ground meat samples were vacuum-packaged in oxygen-impermeable vacuum bags, cooked in-bag to the internal temperature of 75 °C, re-packaged in oxygen-permeable bags, and then stored. Both raw and cooked meats were analyzed for lipid and protein oxidation, color, and volatiles (cooked meat only at 0, 3, and 7 days of storage. Raw meats with 10 ppm of TA added had significantly (p ≤ 0.05 lower lipid and protein oxidation than other treatments during storage. In addition, TA at 10 ppm level maintained the highest color a*- and L*-values during storage. Cooked chicken breast meat with 5 and 10 ppm TA added produced significantly (p ≤ 0.05 lower amounts of off-odor volatiles than other treatments. Among the volatile compounds, the amount of hexanal increased rapidly during storage for cooked meat. However, meats with 5 and 10 ppm TA added showed the lowest amount of hexanal and other aldehydes related to lipid oxidation, indicating a strong antioxidant effect of TA in cooked chicken breast meat. Furthermore, the differences in aldehydes among the treatments were bigger in cooked than in raw meat, indicating that the antioxidant effect of TA in cooked meat was greater than that in raw meat. Therefore, TA at >5 ppm can be used as a good natural preservative in cooked chicken meat to maintain its quality during storage.

  7. Identification and characterization of finger millet OPAQUE2 transcription factor gene under different nitrogen inputs for understanding their role during accumulation of prolamin seed storage protein.

    Science.gov (United States)

    Gaur, Vikram Singh; Kumar, Lallan; Gupta, Supriya; Jaiswal, J P; Pandey, Dinesh; Kumar, Anil

    2018-03-01

    In this study, we report the isolation and characterization of the mRNA encoding OPAQUE2 (O2) like TF of finger millet (FM) ( Eleusine coracana) ( EcO2 ). Full-length EcO2 mRNA was isolated using conserved primers designed by aligning O2 mRNAs of different cereals followed by 3' and 5' RACE (Rapid Amplification of cDNA Ends). The assembled full-length EcO2 mRNA was found to contain an ORF of 1248-nt coding the 416 amino acids O2 protein. Domain analysis revealed the presence of the BLZ and bZIP-C domains which is a characteristic feature of O2 proteins. Phylogenetic analysis of EcO2 protein with other bZIP proteins identified using finger millet transcriptome data and O2 proteins of other cereals showed that EcO2 shared high sequence similarity with barley BLZ1 protein. Transcripts of EcO2 were detected in root, stem, leaves, and seed development stages. Furthermore, to investigate nitrogen responsiveness and the role of EcO2 in regulating seed storage protein gene expression, the expression profiles of EcO2 along with an α-prolamin gene were studied during the seed development stages of two FM genotypes (GE-3885 and GE-1437) differing in grain protein content (13.8 and 6.2%, respectively) grown under increasing nitrogen inputs. Compared to GE-1437, the EcO2 was relatively highly expressed during the S2 stage of seed development which further increased as nitrogen input was increased. The Ecα - prolamin gene was strongly induced in the high protein genotype (GE-3885) at all nitrogen inputs. These results indicate the presence of nitrogen responsiveness regulatory elements which might play an important role in accumulating protein in FM genotypes through modulating EcO2 expression by sensing plant nitrogen status.

  8. Improved platelet survival after cold storage by prevention of glycoprotein Ibα clustering in lipid rafts

    NARCIS (Netherlands)

    Gitz, E.; Koekman, C.A.; van den Heuvel, D.J.|info:eu-repo/dai/nl/355331861; Deckmyn, H.; Akkerman, J.W.N.; Gerritsen, H.C.|info:eu-repo/dai/nl/071548777; Urbanus, R.T

    2012-01-01

    ABSTRACT Background Room temperature storage of platelets for transfusion increases the risk of microbial infection and decreases platelet functionality, leading to out-date discard rates of up to 20%. Cold storage may be a better alternative, but this treatment leads to rapid platelet clearance

  9. TALEN-Based Mutagenesis of Lipoxygenase LOX3 Enhances the Storage Tolerance of Rice (Oryza sativa Seeds.

    Directory of Open Access Journals (Sweden)

    Lei Ma

    Full Text Available The deterioration of rice grain reduces the quality of rice, resulting in serious economic losses for farmers. Lipoxygenases (LOXs catalyze the dioxygenation of polyunsaturated fatty acids with at least one cis,cis-1,4-pentadiene to form hydroperoxide, which is a major factor influencing seed longevity and viability. Recently, genome editing, an essential tool employed in reverse genetics, has been used experimentally to investigate basic plant biology or to modify crop plants for the improvement of important agricultural traits. In this study, we performed targeted mutagenesis in rice using transcription activator-like effector nucleases (TALENs to improve seed storability. A modified ligation-independent cloning method (LIC was employed to allow for the quick and efficient directional insertion of TALEN monomer modules into destination vectors used in plants. We demonstrated the feasibility and flexibility of the technology by developing a set of modular vectors for genome editing. After construction and validation, the TALEN pairs were used to create stable transgenic rice lines via Agrobacterium-mediated transformation. One heterozygous mutant (4% was recovered from 25 transgenic NPTII-resistant lines, and the mutation was transmitted to the next generation. Further molecular and protein level experiments verified LOX3 deficiency and demonstrated the improvement of seed storability. Our work provides a flexible genome editing tool for improving important agronomic traits, as well as direct evidence that Lox3 has only a limited impact on seed longevity.

  10. TALEN-Based Mutagenesis of Lipoxygenase LOX3 Enhances the Storage Tolerance of Rice (Oryza sativa) Seeds.

    Science.gov (United States)

    Ma, Lei; Zhu, Fugui; Li, Zhenwei; Zhang, Jianfu; Li, Xin; Dong, Jiangli; Wang, Tao

    2015-01-01

    The deterioration of rice grain reduces the quality of rice, resulting in serious economic losses for farmers. Lipoxygenases (LOXs) catalyze the dioxygenation of polyunsaturated fatty acids with at least one cis,cis-1,4-pentadiene to form hydroperoxide, which is a major factor influencing seed longevity and viability. Recently, genome editing, an essential tool employed in reverse genetics, has been used experimentally to investigate basic plant biology or to modify crop plants for the improvement of important agricultural traits. In this study, we performed targeted mutagenesis in rice using transcription activator-like effector nucleases (TALENs) to improve seed storability. A modified ligation-independent cloning method (LIC) was employed to allow for the quick and efficient directional insertion of TALEN monomer modules into destination vectors used in plants. We demonstrated the feasibility and flexibility of the technology by developing a set of modular vectors for genome editing. After construction and validation, the TALEN pairs were used to create stable transgenic rice lines via Agrobacterium-mediated transformation. One heterozygous mutant (4%) was recovered from 25 transgenic NPTII-resistant lines, and the mutation was transmitted to the next generation. Further molecular and protein level experiments verified LOX3 deficiency and demonstrated the improvement of seed storability. Our work provides a flexible genome editing tool for improving important agronomic traits, as well as direct evidence that Lox3 has only a limited impact on seed longevity.

  11. Relationships among chilling hours, photoperiod, calendar date, cold hardiness, seed source, and storage of Douglas-fir seedlings

    Science.gov (United States)

    Diane L. Haase; Nabil Khadduri; Euan Mason; Kas Dumroese

    2016-01-01

    Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) seedlings from three nurseries in the Pacific Northwest United States were lifted on five dates from mid-October through mid-December 2006. Each nursery provided seedlings from a low- and a high-elevation seed lot. Photoperiod and accumulated chilling hours (calculated using two methods) were evaluated...

  12. Essential role of hormone-sensitive lipase (HSL) in the maintenance of lipid storage in Mycobacterium leprae-infected macrophages.

    Science.gov (United States)

    Tanigawa, Kazunari; Degang, Yang; Kawashima, Akira; Akama, Takeshi; Yoshihara, Aya; Ishido, Yuko; Makino, Masahiko; Ishii, Norihisa; Suzuki, Koichi

    2012-05-01

    Mycobacterium leprae (M. leprae), the causative agent of leprosy, parasitizes within the foamy or enlarged phagosome of macrophages where rich lipids accumulate. Although the mechanisms for lipid accumulation in the phagosome have been clarified, it is still unclear how such large amounts of lipids escape degradation. To further explore underlying mechanisms involved in lipid catabolism in M. leprae-infected host cells, we examined the expression of hormone-sensitive lipase (HSL), a key enzyme in fatty acid mobilization and lipolysis, in human macrophage THP-1 cells. We found that infection by live M. leprae significantly suppressed HSL expression levels. This suppression was not observed with dead M. leprae or latex beads. Macrophage activation by peptidoglycan (PGN), the ligand for toll-like receptor 2 (TLR2), increased HSL expression; however, live M. leprae suppressed this increase. HSL expression was abolished in the slit-skin smear specimens from patients with lepromatous and borderline leprosy. In addition, the recovery of HSL expression was observed in patients who experienced a lepra reaction, which is a cell-mediated, delayed-type hypersensitivity immune response, or in patients who were successfully treated with multi-drug therapy. These results suggest that M. leprae suppresses lipid degradation through inhibition of HSL expression, and that the monitoring of HSL mRNA levels in slit-skin smear specimens may be a useful indicator of patient prognosis.

  13. Deterioração de sementes de mamona armazenadas com e sem casca Deterioration of shelled and not shelled castorbean seeds during storage

    Directory of Open Access Journals (Sweden)

    Antonio Augusto do Lago

    1985-01-01

    and 75%, 76 and 62%, 68 and 13%, and 38 and 0%, after the same storage periods. After 36 months of storage, the free fatty acid contents for not shelled seeds of 'Campinas' and 'Guarani' cultivars were respectively, 1.6 and 3.2%, whereas for shelled seeds of the same cultivars, the contents were 63.0 and 64.8%, respectively.

  14. A novel mutation in PNPLA2 causes neutral lipid storage disease with myopathy and triglyceride deposit cardiomyovasculopathy: a case report and literature review.

    Science.gov (United States)

    Kaneko, Kimihiko; Kuroda, Hiroshi; Izumi, Rumiko; Tateyama, Maki; Kato, Masaaki; Sugimura, Koichiro; Sakata, Yasuhiko; Ikeda, Yoshihiko; Hirano, Ken-Ichi; Aoki, Masashi

    2014-07-01

    Mutations in PNPLA2 cause neutral lipid storage disease with myopathy (NLSDM) or triglyceride deposit cardiomyovasculopathy (TGCV). We report a 59-year-old patient with NLSDM/TGCV presenting marked asymmetric skeletal myopathy and cardiomyovasculopathy. Skeletal muscle and endomyocardial biopsies showed cytoplasmic vacuoles containing neutral lipid. Gene analysis revealed a novel homozygous mutation (c.576delC) in PNPLA2. We reviewed 37 genetically-proven NLSDM/TGCV cases; median age was 30 years; distribution of myopathy was proximal (69%) and distal predominant (16%); asymmetric myopathy (right>left) was reported in 41% of the patients. Frequently-affected muscles were posterior compartment of leg (75%), shoulder girdle to upper arm (50%), and paraspinal (33%). Skeletal muscle biopsies showed lipid accumulation in 100% and rimmed vacuoles in 22%. Frequent comorbidities were cardiomyopathy (44%), hyperlipidemia (23%), diabetes mellitus (24%), and pancreatitis (14%). PNPLA2 mutations concentrated in Exon 4-7 without apparent genotype-phenotype correlations. To know the characteristic features is essential for the early diagnosis of NLSDM/TGCV. Copyright © 2014 Elsevier B.V. All rights reserved.

  15. Avaliação da qualidade das sementes de Moringa oleifera lam. durante o armazenamento Evaluation of quality of the drumstick seeds during the storage

    Directory of Open Access Journals (Sweden)

    Antonio Marcos Esmeraldo Bezerra

    2004-12-01

    Full Text Available A moringa (Moringa oleifera Lam.; Moringaceae, espécie perene originária do noroeste indiano, que se adapta tanto às condições irrigadas quanto às de sequeiro, pouco exigente em solos e fertilizantes, encontra-se disseminada na região nordeste, particularmente no Ceará, graças à sua utilização na clarificação de águas turvas. A despeito do seu reconhecido valor medicinal e como hortaliça não-convencional rica em vitamina A, ainda carece de estudos que focalizem a viabilidade das sementes durante o armazenamento. Baseado nessa premissa, conduziu-se um ensaio com os tratamentos dispostos num arranjo fatorial 2x4, correspondendo à combinação de dois ambientes (natural e câmara fria e quatro tempos de armazenamento (0, 6, 12 e 24 meses, segundo um delineamento inteiramente casualizado com quatro repetições. As amostras de sementes correspondentes aos tratamentos investigados foram acondicionadas em garrafa plásticas tipo PET de 600 mL. As variáveis de observação foram: teor de água, germinação, comprimento da raiz, massa seca de plântula e condutividade elétrica. Pela análise dos resultados, evidenciou-se que, aos 12 meses de armazenamento em embalagem plástica sob ambiente natural, as sementes perdem a sua viabilidade, enquanto na câmara aos 24 meses, as sementes apresentam redução na qualidade fisiológica.Drumstick (Moringa oleifera Lam., Moringaceae is a perennial species originating from the indian northwest that adapts to both irrigated and rain fed crop conditions and it is not very demanding on soils and fertilizers. It is grown in the brazilian northeast, specially in Ceará State, due to its use in clarification of cloudy waters. In spite of its recognized medicinal value and as non conventional vegetable rich in vitamin A no studies focalizing the viability of the seeds during the storage have been done. So, an experiment was carried out treatments arranged in a fatorial 2x4 scheme, corresponding the

  16. Analysis of hepatic transcript profile and plasma lipid profile in early lactating dairy cows fed grape seed and grape marc meal extract.

    Science.gov (United States)

    Gessner, Denise K; Winkler, Anne; Koch, Christian; Dusel, Georg; Liebisch, Gerhard; Ringseis, Robert; Eder, Klaus

    2017-03-23

    It was recently reported that dairy cows fed a polyphenol-rich grape seed and grape marc meal extract (GSGME) during the transition period had an increased milk yield, but the underlying reasons remained unclear. As polyphenols exert a broad spectrum of metabolic effects, we hypothesized that feeding of GSGME influences metabolic pathways in the liver which could account for the positive effects of GSGME in dairy cows. In order to identify these pathways, we performed genome-wide transcript profiling in the liver and lipid profiling in plasma of dairy cows fed GSGME during the transition period at 1 week postpartum. Transcriptomic analysis of the liver revealed 207 differentially expressed transcripts, from which 156 were up- and 51 were down-regulated, between cows fed GSGME and control cows. Gene set enrichment analysis of the 155 up-regulated mRNAs showed that the most enriched gene ontology (GO) biological process terms were dealing with cell cycle regulation and the most enriched Kyoto Encyclopedia of Genes and Genomes pathways were p53 signaling and cell cycle. Functional analysis of the 43 down-regulated mRNAs revealed that a great part of these genes are involved in endoplasmic reticulum (ER) stress-induced unfolded protein response (UPR) and inflammatory processes. Accordingly, protein folding, response to unfolded protein, unfolded protein binding, chemokine activity and heat shock protein binding were identified as one of the most enriched GO biological process and molecular function terms assigned to the down-regulated genes. In line with the transcriptomics data the plasma concentrations of the acute phase proteins serum amyloid A (SAA) and haptoglobin were reduced in cows fed GSGME compared to control cows. Lipidomic analysis of plasma revealed no differences in the concentrations of individual species of major and minor lipid classes between cows fed GSGME and control cows. Analysis of hepatic transcript profile in cows fed GSGME during the

  17. Proteome analysis reveals an energy-dependent central process for Populus×canadensis seed germination.

    Science.gov (United States)

    Zhang, Hong; Zhou, Ke-Xin; Wang, Wei-Qing; Liu, Shu-Jun; Song, Song-Quan

    2017-06-01

    Poplar (Populus×canadensis) seeds rapidly germinated in darkness at 10, 15, and 20°C and reached 50% seed germination after about 22, 4.5, and 3.5h, respectively. Germination of poplar seeds was markedly inhibited by abscisic acid (ABA) at 50μM and cycloheximide (CHX) at 100μM, and these inhibitive roles were temperature-dependent. In the present study, mature poplar seeds were used to investigate the differentially changed proteome of seeds germinating in water, ABA, and CHX. A total of 130 protein spots showed a significant change (1.5-fold increase/decrease, Pgermination of poplar seeds is closely related with the increase in those proteins involved in amino acid and lipid metabolism, the tricarboxylic acid cycle and pentose phosphate pathway, protein synthesis and destination, cell defense and rescue, and degradation of storage proteins. ABA and CHX inhibit the germination of poplar seeds by decreasing the protein abundance associated with protein proteolysis, protein folding, and storage proteins. We conclude that poplar seed germination is an energy-dependent active process, and is accompanied by increasing amino acid activation, protein synthesis and destination, as well as cell defense and rescue, and degradation of storage proteins. Copyright © 2017 Elsevier GmbH. All rights reserved.

  18. Controle de fungos presentes no ar e em sementes de feijão durante armazenamento Control of fungi present in the air and bean seeds during storage

    Directory of Open Access Journals (Sweden)

    Flávio M. Borém

    2006-09-01

    Full Text Available Objetivou-se, no presente trabalho, avaliar a eficácia de um equipamento redutor de inóculo (ERI na preservação da qualidade sanitária de sementes de feijão (Phaseolus vulgaris L. durante o armazenamento em estruturas herméticas com circulação de ar forçado. A presença de inóculo no ar foi monitorada a partir da exposição de placas de Petri com meio de cultura BDA salino, contando-se o número de unidades formadoras de colônias (UFC dos fungos Aspergillus spp. e Penicillium spp. As análises sanitárias das sementes foram realizada pelo método do papel de filtro (Blotter test, calculando-se o índice de ocorrência de Aspergillus spp. e Penicillium spp. Durante o período de armazenamento, houve um aumento na incidência de fungos nas sementes, independente da presença dos ERI. Nas amostragens realizadas na entrada da caixa com a presença dos ERI, não foram encontradas unidades formadoras de colônias. Com o aumento do fluxo de ar utilizado no sistema, notou-se que este efeito foi transferido para a saída da caixa. Nas condições especificadas, os ERI foram incapazes de controlar os fungos de armazenamento, Aspergillus spp e Penicillium spp, nas sementes de feijão, porém foram eficazes no controle dos seus esporos presentes no ar.The objective of the present work was to evaluate the efficiency of inoculum reducing equipment (IRE in the maintenance of quality of bean seeds during storage in hermetic structures with circulation of forced air. The inoculum's presence in the air was monitored by the exposition of Petri dishes with culture medium (saline BDA by counting the number of colonies units of Aspergillus and Penicillium. The quality analysis of seeds were performed by the blotter test. The occurrence index was calculated for Aspergillus spp and Penicillium spp. The fungal occurrence increased during the storage period, in spite of the IRE presence. Fungal colonies were not found in the air samples collected at the box

  19. Oxidative stability of refrigerated fish pates containing loquat seed extract

    Directory of Open Access Journals (Sweden)

    Jaqueline Piccolo

    2014-09-01

    Full Text Available This study investigated the effects of hydroethanolic E. japonica seed extracts (EJSE as inhibitors of lipid and protein oxidation on fish pates subjected to refrigerated storage. Five fish pate formulations were developed. These formulations included two control pates (water-control and ascorbic acid-control and three pates with added EJSE (0.1, 0.2 and 0.4g of seed 100g-1 product, equivalent to 3.4, 6.8 or 13.6mg phenolic compounds kg-1 product, which were then stored under refrigeration for 35 days. Conjugated dienes (CD and peroxide (PV values increased along with the storage time; however, these values decreased and were similar among all samples at the end of 35 days of analysis (P<0.05. However, the thiobarbituric acid reactive substances levels (TBARS did not change along the storage and were not affected by the EJSE. Additionally, there was a linear increase in the protein carbonyl content of fish pates over the storage period (P<0.05, but no effect of EJSE on protein oxidation. The results show that, at the concentrations evaluated, hydroethanolic E. japonica seed extract was unable to inhibit or reduce lipid and protein oxidation in fish pates, but the observed phenolic content emphasizes the need for further studies on the wastes of this fruit.

  20. Effects of dietary grape seed extract on growth performance, amino acid digestibility and plasma lipids and mineral content in broiler chicks.

    Science.gov (United States)

    Chamorro, S; Viveros, A; Centeno, C; Romero, C; Arija, I; Brenes, A

    2013-04-01

    Polyphenols are chemically and biologically active compounds. Grape seed extracts (GSEs) have been widely used as a human food supplement for health promotion and disease prevention. However, there is little information regarding its application in animal feeds. An experiment was conducted to investigate the effect of inclusion of GSE at 0.025, 0.25, 2.5 and 5.0 g/kg in a wheat soya bean control diet on growth performance, protein and amino acid (AA) digestibility and plasma lipid and mineral concentrations in broiler chickens at 21 days of age. Performance was not affected by dietary treatment except in the case of birds fed the diet with the highest GSE concentration, which showed a worsening of weight gain and feed conversion. Apparent ileal digestibility (AID) of protein was significantly reduced in the birds fed the highest concentration of GSE, which also had a reduction on the AID of arginine, histidine, phenylalanine, cystine, glutamic acid and proline compared with those fed control diet. The inclusion of graded concentration of GSE in the chicken diets caused a significant linear decrease in the concentrations of plasma copper, iron and zinc. Plasma cholesterol, triglycerides and lipoproteins (high-density lipoprotein, low-density lipoprotein and very-low-density lipoprotein) concentrations were not affected by dietary GSE. In conclusion, this study demonstrated that incorporation of GSE in chicken diets up to 2.5 g/kg had no adverse effect on growth performance or protein and AA digestibility. Feed conversion was reduced and growth rate was retarded, when chickens were fed 5 g/kg of GSE. This study also indicated that grape polyphenols reduce the free plasma minerals.

  1. Germinação e armazenamento de sementes de Virola surinamensis (Rol. Warb. (Myristicaceae Germination and storage of seeds of Virola surinamensis (Rol. Warb. (Myristicaceae

    Directory of Open Access Journals (Sweden)

    Juliana Domingues Limas

    2007-02-01

    Full Text Available Este trabalho teve como objetivo estudar a germinação e o armazenamento de sementes de Virola surinamensis (Rol. Warb. em condições de laboratório, no Estado do Amapá, Brasil. Para tanto, frutos foram coletados na Reserva Particular de Patrimônio Natural, "Ekinox", localizada em Macapá. Sementes recém-colhidas do lote original apresentaram teor médio de água de 24% e baixo ganho de água durante a embebição. Devido às diferenças no tamanho e, ou, peso, as sementes colhidas foram divididas em dois grupos: grandes e pequenas. Independentemente do tamanho da semente, a maior porcentagem de germinação e o menor tempo médio de germinação ocorreram a 30 ºC. Sementes armazenadas por 30 dias em condição ambiente (27 ºC ± 3 e 75% ± 5 UR e em germinador (20 ºC e 58% UR apresentaram viabilidade abaixo de 2%, ressaltando-se o comportamento recalcitrante de sementes de Virola surinamensis.The objective of this work was to study the germination and the storage of seeds of Virola surinamensis (Rol. Warb. in laboratory conditions, in the State of Amapa, Brazil. Fruits were harvested in the "Ekinox" Private Reserve of Natural Patrimony, located at Macapa. Seeds recently harvested in the original seedlot showed 24% mean water content and lower water gain, during soaking. Due to differences in the size and/or weight, the seeds were separated in two groups: large and small. Independently of seed size, the highest germination percentage and the lower average germination time occurred at 30ºC. Seeds stored under natural (27 ºC ± 3 e 75% ± 5 RU or germination chamber conditions (20 ºC e 58% RU showed viability lower than 2%, attesting the recalcitrant behavior of Virola surinamensis.

  2. Maturity and storage influence on the apple (Malus domestica) allergen Mal d 3, a nonspecific lipid transfer protein

    NARCIS (Netherlands)

    Sancho, Ana I.; Foxall, Robert; Rigby, Neil M.; Browne, Thomas; Zuidmeer, Laurian; van Ree, Ronald; Waldron, Keith W.; Mills, E. N. Clare

    2006-01-01

    Consumption of apples can provoke severe allergic reactions, in susceptible individuals, due to the presence of the allergen Mal d 3, a nonspecific lipid transfer protein, found largely in the fruit skin. Levels of Mal d 3 were determined in peel as a function of apple cultivar, position of the

  3. Antioxidative Effect of Seaweed Extracts in Chilled Storage of Minced Atlantic Mackerel (Scomber scombrus): Effect on Lipid and Protein Oxidation

    DEFF Research Database (Denmark)

    Babakhani, Aria; Farvin, K. H Sabeena; Jacobsen, Charlotte

    2016-01-01

    In this study, antioxidant activity of absolute ethanol, 50 % ethanol and water extracts of two species of seaweeds namely, Fucus serratus and Polysiphonia fucoides were evaluated for their ability to retard lipid and protein oxidation in minced mackerel. Mackerel mince added with 0.5 g/kg of ext...

  4. Equilibrium relative humidity as a tool to monitor seed moisture

    Science.gov (United States)

    Robert P. Karrfalt

    2010-01-01

    The importance of seed moisture in maintaining high seed viability is well known. The seed storage chapters in the Tropical Tree Seed Manual (Hong and Ellis 2003) and the Woody Plant Seed Manual (Bonner 2008a) give a detailed discussion and many references on this point. Working with seeds in an operational setting requires a test of seed moisture status. It is...

  5. Effects of dietary vanadium on growth and lipid storage in a larval anuran: Results from studies employing ad libitum and rationed feeding

    International Nuclear Information System (INIS)

    Rowe, Christopher L.; Heyes, Andrew; Hopkins, William

    2009-01-01

    Vanadium (V) exerts a variety of effects related to metabolic function in vertebrates, including modifying glycolytic pathways and lipid metabolism. However, little is known about toxicity of V to wildlife in natural systems. We conducted parallel, independent studies to evaluate the effect of dietary exposure to vanadium on survival, metabolism, growth, and lipid storage of larval leopard frogs (Rana sphenocephala). In one study ('Ad lib') larvae were fed unlimited food and in the other ('Ration') larvae were fed a restricted diet. Experimental diets contained 10 ppm dw V total (Control, 'C'), 109 ppm (Low, 'L'), and 363 ppm (High, 'H'). In neither study did V affect metabolic rate or survival to forelimb emergence. However, in the Ration study, 31% of individuals in H that had initiated metamorphosis died prior to completion of metamorphosis, compared with 22% in M and 9% in C, however the difference was not significant at α = 0.05. Mortality during metamorphosis in the Ad lib study was more variable, yet followed a similar trend (26, 14, and 12% mortality in H, L, and C). In both studies, individuals that died during the metamorphic period were smaller at the time of forelimb emergence than those that survived. In the Ration study, individuals in H also had significantly lower growth rates, delayed metamorphosis, and reduced lipid content at metamorphosis compared to C and L. Growth was not affected by V in the Ad lib study. Our results suggest that habitat contamination by V may present risks to amphibians during the metamorphic period attributable to energetic responses influencing size at the initiation of metamorphosis and body lipid stores. Furthermore, while we cannot make formal comparisons between the studies, our results imply that protocols that employ ad libitum feeding regimes could fail to capture the effects of contaminants as they may be expressed in natural situations in which resources are typically more limited

  6. Effects of dietary vanadium on growth and lipid storage in a larval anuran: Results from studies employing ad libitum and rationed feeding

    Energy Technology Data Exchange (ETDEWEB)

    Rowe, Christopher L. [University of Maryland Center for Environmental Science, Chesapeake Biological Laboratory, P.O. Box 38, Solomons, MD 20688 (United States)], E-mail: Rowe@cbl.umces.edu; Heyes, Andrew [University of Maryland Center for Environmental Science, Chesapeake Biological Laboratory, P.O. Box 38, Solomons, MD 20688 (United States); Hopkins, William [Department of Fisheries and Wildlife Sciences, Virginia Polytechnic Institute and State University, 100 Cheatham Hall, Blacksburg, VA 24061 (United States)

    2009-01-31

    Vanadium (V) exerts a variety of effects related to metabolic function in vertebrates, including modifying glycolytic pathways and lipid metabolism. However, little is known about toxicity of V to wildlife in natural systems. We conducted parallel, independent studies to evaluate the effect of dietary exposure to vanadium on survival, metabolism, growth, and lipid storage of larval leopard frogs (Rana sphenocephala). In one study ('Ad lib') larvae were fed unlimited food and in the other ('Ration') larvae were fed a restricted diet. Experimental diets contained 10 ppm dw V{sub total} (Control, 'C'), 109 ppm (Low, 'L'), and 363 ppm (High, 'H'). In neither study did V affect metabolic rate or survival to forelimb emergence. However, in the Ration study, 31% of individuals in H that had initiated metamorphosis died prior to completion of metamorphosis, compared with 22% in M and 9% in C, however the difference was not significant at {alpha} = 0.05. Mortality during metamorphosis in the Ad lib study was more variable, yet followed a similar trend (26, 14, and 12% mortality in H, L, and C). In both studies, individuals that died during the metamorphic period were smaller at the time of forelimb emergence than those that survived. In the Ration study, individuals in H also had significantly lower growth rates, delayed metamorphosis, and reduced lipid content at metamorphosis compared to C and L. Growth was not affected by V in the Ad lib study. Our results suggest that habitat contamination by V may present risks to amphibians during the metamorphic period attributable to energetic responses influencing size at the initiation of metamorphosis and body lipid stores. Furthermore, while we cannot make formal comparisons between the studies, our results imply that protocols that employ ad libitum feeding regimes could fail to capture the effects of contaminants as they may be expressed in natural situations in which resources

  7. Volatile emission in dry seeds as a way to probe chemical reactions during initial asymptomatic deterioration.

    Science.gov (United States)

    Mira, Sara; Hill, Lisa M; González-Benito, M Elena; Ibáñez, Miguel Angel; Walters, Christina

    2016-03-01

    The nature and kinetics of reactions in dry seeds determines how long the seeds survive. We used gas chromatography to assay volatile organic compounds (VOCs) emitted from seeds of three unrelated species as a means to non-invasively probe chemical changes during very dry, dry, and humid storage (seeds were dried to 5.5, 33, and 75% relative humidity at room temperature). VOCs emitted from seeds stored in humid conditions reflected fermentation-type reactions, with methanol and ethanol being predominant in Lactuca sativa and Carum carvi, and acetaldehyde and acetone being predominant in Eruca vesicaria. Dried C. carvi seeds continued to emit fermentation-type products, although at slower rates than the seeds stored in humid conditions. In contrast, drying caused a switch in VOC emission in L. sativa and E. vesicaria seeds towards higher emission of pentane and hexanal, molecules considered to be byproducts from the peroxidation of polyunsaturated fatty acids. Longevity correlated best with the rate of fermentation-type reactions and appeared unrelated to the rate of lipid peroxidation. Emission of VOCs decreased when seed species were mixed together, indicating that seeds adsorbed VOCs. Adsorption of VOCs did not appear to damage seeds, as longevity was not affected in seed mixtures. Collectively, the study shows similarity among species in the types of reactions that occur in dry seeds, but high diversity in the substrates, and hence the byproducts, of the reactions. Moreover, the study suggests that the most abundant VOCs arise from degradation of storage reserves within seed cells, and that these reactions and their byproducts are not, in themselves, damaging. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  8. Viabilidade de sementes de erva-de-touro, sob diferentes condições de armazenamento Seed viability of coat button under different storage conditions

    Directory of Open Access Journals (Sweden)

    S.C. Guimarães

    2004-06-01

    Full Text Available A manutenção da viabilidade das sementes é muito influenciada pelas condições de armazenamento. No caso das plantas daninhas, essa informação pode servir como suporte para a realização de outras pesquisas e também ajudar na compreensão da dinâmica das infestações. Neste trabalho, a viabilidade das sementes da planta daninha erva-de-touro (Tridax procumbens foi monitorada durante dois anos, quando armazenadas em câmara fria (temperatura de 10 ºC e umidade relativa de 50%, em congelador (-18 ºC, em armazém convencional (condições não controladas e no solo. Quando armazenado em câmara fria (em sacos de papel e em congelador (em tubos plásticos herméticos, o lote de sementes mantém a viabilidade inicial (70,5% por no mínimo 730 dias (período experimental. No solo, ocorre perda de viabilidade com o tempo, numa taxa constante de 8,2% para cada 100 dias. Em armazém convencional, a viabilidade das sementes (em sacos de papel é mantida por 200 dias, com redução acentuada entre 300 e 500 dias, chegando ao final de 730 dias com 2,8% de viabilidade. Em nenhuma das formas de armazenamento há indução de dormência secundária nas sementes.Seed viability is strongly influenced by storage conditions. In weeds, this information may serve as support for other research works and help to understand infestation dynamics as well. This work, conducted at Universidade Federal de Lavras, was carried out to evaluate the viability of coat button (Tridax procumbens seeds stored in cold chamber ( temperature of 10 ºC and relative humidity of 50%, in freezer (-18 ºC, conventional warehouse (uncontrolled conditions and soil, over 2 years. Seed lot with 70.5% initial viability, maintained this condition over the experimental period (730 days when stored in cold chamber (in paper bags and in freezer (in airtight plastic tubes. Under soil conditions, viability loss occurred with time, at a constant rate of 8.2% at every 100 days. In a

  9. Oxidative stress induction by Prunus necrotic ringspot virus infection in apricot seeds.

    Science.gov (United States)

    Amari, Khalid; Díaz-Vivancos, Pedro; Pallás, Vincente; Sánchez-Pina, María Amelia; Hernández, José Antonio

    2007-10-01

    Prunus necrotic ringspot rvirus (PNRSV) was able to invade the immature apricot seed including the embryo. The amount of virus was very high inside the embryo compared with that present in the cotyledons. PNRSV infection produced an oxidative stress in apricot seeds as indicated by the increase in lipid peroxidation, measured as thiobarbituric acid-reactive substances. This lipid peroxidation increase was parallelled with an imbalance in the seed antioxidant enzymes. A significant decrease in the ascorbate-GSH cycle enzymes as well as in peroxidase (POX) activity took place in infected seeds, suggesting a low capability to eliminate H2O2. No changes in superoxide dismutase (SOD) or catalase activity were observed. A significant decrease in polyphenoloxidase (PPO) activity was also observed. Native PAGE revealed the presence of three different SOD activity bands in apricot seeds: a Mn-containing SOD and two CuZn-containing SODs. Only an isozyme with catalase, glutathione reductase (GR) or PPO activity was detected in both healthy and infected apricot seeds. Regarding POX staining, three bands with POX activity were detected in native gels in both healthy and infected seeds. The gel results emphasise that the drop detected in POX, GR and PPO activities in PNRSV-infected apricot seeds by kinetic analyses was also evident from the results obtained by native PAGE. The oxidative stress and the imbalance in the antioxidant systems from PNRSV-infected apricot seeds resemble the hypersensitive response observed in some virus-host interactions. This defence mechanism would inactivate PNRSV during seed formation and/or the storage period or even during seed germination. Those results can explain the decrease in seed germination and the low transmission of PNRSV by seeds in apricot trees.

  10. Effects of hydroalcoholic extract of Rhus coriaria seed on glucose and insulin related biomarkers, lipid profile, and hepatic enzymes in nicotinamide-streptozotocin-induced type II diabetic male mice.

    Science.gov (United States)

    Ahangarpour, Akram; Heidari, Hamid; Junghani, Majid Salehizade; Absari, Reza; Khoogar, Mehdi; Ghaedi, Ehsan

    2017-10-01

    Type 2 diabetes often leads to dislipidemia and abnormal activity of hepatic enzymes. The purpose of this study was to evaluate the antidiabetic and hypolipidemic properties of Rhus coriaria ( R. coriaria ) seed extrac on nicotinamide-streptozotocin induced type 2 diabetic mice. In this experimental study, 56 male Naval Medical Research Institute mice (30-35 g) were randomly separated into seven groups: control, diabetic group, diabetic mice treated with glibenclamide (0.25 mg/kg, as standard antidiabetic drug) or R. coriaria seed extract in doses of 200 and 300 mg/kg, and control groups received these two doses of extract orally for 28 days. Induction of diabetes was done by intraperitoneal injection of nicotinamide and streptozotocin. Ultimately, body weight of mice, blood levels of glucose, insulin, hepatic enzymes, leptin, and lipid profile were assayed. After induction of type 2 diabetes, level of glucose, cholesterol, low density lipoprotein, serum glutamic oxaloacetic transaminase, and serum glutamic pyruvic transaminase increased and level of insulin and high density lipoprotein decreased remarkably. Administration of both doses of extract decreased level of glucose and cholesterol significantly in diabetic mice. LDL level decreased in treated group with dose of 300 mg/kg of the extract. Although usage of the extract improved level of other lipid profiles, insulin and hepatic enzymes, changes weren't significant. This study showed R. coriaria seeds administration has a favorable effect in controlling some blood parameters in type 2 diabetes. Therefore it may be beneficial in the treatment of diabetes.

  11. Modifying effect of 5-fluoro-2-deoxyuridine on chromosome aberrations yield after the storage of irradiated and nitrogen mustard-treated Crepis capillaris L. seeds in the G1 phase

    International Nuclear Information System (INIS)

    Mirzoyan, G.I.; Azatyan, R.A.; Avakyan, V.A.

    1985-01-01

    Variations in the yield of chromosome structural mutations during storage of irradiated and treated with nitrogen-mustard alkylating agent Crepis capillaris L. seeds are noticed. The data obtained testify to the absence of coincidence between the curve peaks in radiation and chemical mutagenesis

  12. Impact of gamma irradiation on biochemical attributes of jackfruit (Artocarpus heterophyllus L.) seeds during different storage period

    International Nuclear Information System (INIS)

    Adiga, Madhavi; Akshatha, Madhuri Adiga; Chandrashekar, K.R.

    2014-01-01

    Seeds of A. heterophyllus Lam. were exposed to different (0.5-2.5 kGy) doses of gamma radiation and stored for 3 weeks under ambient condition for observation of biochemical changes. The significant increase in phenolic content was observed at 2.5 kGy compared to control. Increase in protein content was observed at 1.5 and 2kGy at the end of 3 rd week. Increased total soluble sugar content was observed with increased dose. Enhancement in antioxidant activity at 2kGy may be due to the increased phenolic content. (author)

  13. Efficacy of chemically characterized Foeniculum vulgare Mill seed essential oil in protection of raw tobacco leaves during storage against fungal and aflatoxin contamination.

    Science.gov (United States)

    Kedia, A; Dwivedy, A K; Pandey, A K; Kumar, R R; Regmi, P; Dubey, N K

    2015-10-01

    To report fungal and aflatoxin contamination in stored tobacco leaves and the potential of Foeniculum vulgare (fennel) seed essential oil (EO) as a plant-based preservative in protection of tobacco during storage. Mycological analysis of tobacco samples was done by surface sterilization and serial dilution tests. The Aspergillus flavus isolates were screened for their toxigenicity. Both in vivo and in vitro tests were done to evaluate antifungal and antiaflatoxigenic efficacy of chemically characterized EO. The mycoflora analysis revealed 108 fungal colonies belonging to five genera and nine species. All A. flavus isolates were found aflatoxigenic during screening. Gas chromatography and mass spectrometry analysis of EO identified 19 components (99·66%); estragole being the major component (47·49%). The EO showed broad fungitoxicity at 1·25 μl ml(-1) and 100% inhibition to AFB1 production as well as ergosterol synthesis at 1·0 μl ml(-1) concentration. EO showed 100% protection of stored tobacco samples from aflatoxin B1 contamination. The fennel EO can thus be formulated as a plant-based preservative for food items. The present investigation comprises the first report on antiaflatoxin efficacy of fennel oil and its potency in the protection of tobacco leaves from fungal and aflatoxin contamination during storage. © 2015 The Society for Applied Microbiology.

  14. Longevidade de sementes de camu-camu submetidas a diferentes ambientes e formas de conservação Longevity camu-camu seeds submitted to different storage environment and forms of conservation

    Directory of Open Access Journals (Sweden)

    Kaoru Yuyama

    2011-06-01

    Full Text Available O camu-camu (Myrciaria dubia (H.B.K. McVaugh produz fruto com grande potencial para extração de ácido ascórbico, que apresenta relevante importância econômica e social, com inúmeras aplicações industriais. O objetivo deste trabalho foi avaliar o efeito de diferentes formas de conservação e ambientes de armazenamento, sobre a manutenção da viabilidade das sementes. O delineamento experimental inteiramente casualizado, utilizando parcelas subsubdivididas, onde os fatores foram: ambiente de armazenamento (ao ar livre, em água, em câmara a 5 e a 10 ºC, forma de conservação (sementes com polpa - CP, sementes sem polpa - SP e sementes lavadas e tratadas - LT e período de armazenamento (0; 2; 4 e 6 meses. O critério de avaliação adotado foi a protrusão da raiz primária. As sementes armazenadas em água, nas formas SP e LT, apresentaram germinação maior que 90%. As sementes nas formas CP e LT, armazenadas a 5 e a 10 ºC, tiveram germinação acima de 89%. O armazenamento em água e ao ar livre não afetou a germinação, podendo as sementes serem armazenadas durante o período de seis e quatro meses, respectivamente. As sementes CP, armazenadas em água, tiveram menor tempo médio para germinação. O índice de velocidade de germinação aumentou com o período de armazenamento e foi maior nas sementes armazenadas na água em todos os períodos. As sementes com polpa apresentaram menor índice de velocidade de germinação em todo o período de armazenamento avaliado.Camu-camu (Myrciaria dubia (H.B.K. McVaugh contains great potential for ascorbic acid extraction, which has economic and social importance, with innumerable industrial applications. The objective of this work was to evaluate the effect of different storage environment and forms of conservation for maintaining the viability of camu-camu seeds. The experimental design was randomized blocks design in split split-plot, with the following factors: storage conditions

  15. Study of the effects of gamma radiation on the microbiological quality, lipid oxidation and sensory properties of mechanically deboned chicken meat throughout refrigerated and frozen storage

    International Nuclear Information System (INIS)

    Gomes, Heliana de Azevedo

    2002-01-01

    Mechanically deboned chicken meat (MDCM) is widely used in traditional meat products such as sausages. The proceed of mechanical separation results in grinding of the bones, liberating the marrow and rupturing cells, thus making the MDCM a favorable medium for biochemical reactions and microbiological growth. Irradiation using a Co 60 source is one of the processes which has been developed aimed at reducing contamination by spoilage and pathogenic microorganisms in foods. The main advantages of food irradiation are the possibilities of treating the already packaged products at refrigerated and frozen temperatures, ideal for a highly perishable product such as MDCM, which, being a ground, easily spoiled product, requires rapid cooling followed by freezing, immediately after processing. Processing with ionizing radiation results in chemical alterations in the food, including the production of volatile compounds and free radicals. Irradiation of meat in the frozen state reduces or eliminates the negative sensory effects of this process. In this context, this study aimed at evaluating the effects of gamma radiation at doses of 3.0 and 4.0 kGy, on the microbiological quality, lipid oxidation and sensory properties of MDCM, at the refrigerated (2 ± 1 deg C) and frozen (-18 ± 1 deg C) storage, The results showed that irradiation increased the shelf life of this raw material when stored under refrigeration, as compared to non-irradiated samples. Since doses of 3.0 kGy produced increases in the shelf life of refrigerated MDCM equal or greater than those produced by doses of 4.0 kGy, this dose was considered the most adequate for the irradiation of this raw material. According to the results of microbiological, chemical and sensorial testing in irradiated MDCM samples with doses of 3.0 kGy and 4.0 kGy, the material studied presented conditions that were adequate for human consumption during the 90 days of frozen storage, whereas samples of non-irradiated MDCM were shown to

  16. Perspectives on marine zooplankton lipids

    DEFF Research Database (Denmark)

    Kattner, G.; Hagen, W.; Lee, R.F.

    2007-01-01

    We developed new perspectives to identify important questions and to propose approaches for future research on marine food web lipids. They were related to (i) structure and function of lipids, (ii) lipid changes during critical life phases, (iii) trophic marker lipids, and (iv) potential impact...... of climate change. The first addresses the role of lipids in membranes, storage lipids, and buoyancy with the following key question: How are the properties of membranes and deposits affected by the various types of lipids? The second deals with the importance of various types of lipids during reproduction......, development, and resting phases and addresses the role of the different storage lipids during growth and dormancy. The third relates to trophic marker lipids, which are an important tool to follow lipid and energy transfer through the food web. The central question is how can fatty acids be used to identify...

  17. Black Seed Thymoquinone Improved Insulin Secretion, Hepatic Glycogen Storage, and Oxidative Stress in Streptozotocin-Induced Diabetic Male Wistar Rats

    Directory of Open Access Journals (Sweden)

    Heba M. A. Abdelrazek

    2018-01-01

    Full Text Available Diabetes mellitus is one of the metabolic diseases having several complications. Nigella sativa oil (NSO might have beneficial effects in the treatment of diabetic complications. Thirty-two mature male Wistar rats were equally divided into four experimental groups: control, control NSO 2 mL/kg, streptozotocin- (STZ- induced diabetic, and diabetic (STZ-induced treated with oral NSO 2 mg/kg for 30 days. Fasting blood glucose (FBG, insulin, and lipid profile levels were determined. Pancreatic and hepatic tissues were used for catalase and GSH. Histopathology, hepatic glycogen contents, insulin immunohistochemistry, and pancreatic islet morphometry were performed. NSO 2 mL/kg was noticed to decrease (P<0.05 FBG and increase (P<0.05 insulin levels in diabetic rats than in diabetic nontreated animals. Lipid profile showed significant (P<0.5 improvement in diabetic rats that received NSO 2 mL/kg than in the diabetic group. Both pancreatic and hepatic catalase and GSH activities revealed a significant (P<0.05 increment in the diabetic group treated with NSO than in the diabetic animals. NSO improved the histopathological picture and hepatic glycogen contents of the diabetic group as well as increased (P<0.05 insulin immunoreactive parts % and mean pancreatic islet diameter. NSO exerts ameliorative and therapeutic effects on the STZ-induced diabetic male Wistar rats.

  18. Wrinkled1 accelerates flowering and regulates lipid homeostasis between oil accumulation and membrane lipid anabolism in Brassica napus

    Directory of Open Access Journals (Sweden)

    Qing eLi

    2015-11-01

    Full Text Available Wrinkled1 (WRI1 belongs to the APETALA2 transcription factor family; it is unique to plants and is a central regulator of oil synthesis in Arabidopsis. The effects of WRI1 on comprehensive lipid metabolism and plant development were unknown, especially in crop plants. This study found that BnWRI1 in Brassica napus accelerated flowering and enhanced oil accumulation in both seeds and leaves without leading to a visible growth inhibition. BnWRI1 decreased storage carbohydrates and increased soluble sugars to facilitate the carbon flux to lipid anabolism. BnWRI1 is localized to the nucleus and directly binds to the AW-box at proximal upstream regions of genes involved in fatty acid synthesis and lipid assembly. The overexpression (OE of BnWRI1 resulted in the up-regulation of genes involved in glycolysis, fatty acid synthesis, lipid assembly, and flowering. Lipid profiling revealed increased galactolipid monogalactosyldiacylglycerol (MGDG, digalactosyldiacylglycerol (DGDG, and phosphatidylcholine (PC in the leaves of OE plants, whereas it exhibited a reduced level of the galactolipids DGDG and MGDG and increased levels of PC, phosphatidylethanolamide (PE, and oil (triacylglycerol, TAG in the siliques of OE plants during the early seed development stage. These results suggest that BnWRI1 is important for homeostasis among TAG, membrane lipids and sugars, and thus facilitates flowering and oil accumulation in B. napus.

  19. Assessment of the indicators atherogenic index and lipid preventive score of white brine cheese by buffalo milk after technological processing and storage

    International Nuclear Information System (INIS)

    Ivanova, S.; Nacheva, I.; Miteva, D.

    2011-01-01

    The present investigation had the objective to study the changes in the atherogenic index - AI and the lipid preventive score - LPS as nutritious indicators for assessment of the risk of cardio-vascular diseases, on the basis of the fatty acid composition of white brine cheese by buffalo milk after applying of two methods of preservation – freeze-drying and dry gamma sterilization with 2 and 4 kGy. The analysis was made with the aid of gas chromatograph SHIMADZU 2010. The lyophilized cheese is characterized by a comparatively low AI - 2.59 after lyophilization and is preserved during storage - 2.55. After lyophilization the LPS of the cheese was the highest – 103.70, while after irradiation a decrease of its value was observed up to 97.73 with 2 kGy and 96.91 with 4 kGy. Key words: white brine cheese by buffalo milk, atherogenic index, lipid preventive score, freeze-drying - lyophilization, gamma sterilization

  20. Assessment of the Indicators Atherogenic Index and Lipid Preventive Score of White Brine Cheese by Buffalo Milk after Technological Processing and Storage

    International Nuclear Information System (INIS)

    Ivanova, S.; Nacheva, I.; Miteva, D.

    2011-01-01

    The present investigation had the objective to study the changes in the atherogenic index - AI and the lipid preventive score - LPS as nutritious indicators for assessment of the risk of cardio-vascular diseases, on the basis of the fatty acid composition of white brine cheese by buffalo milk after applying of two methods of preservation – freeze-drying and dry gamma sterilization with 2 and 4 kGy. The analysis was made with the aid of gas chromatograph SHIMADZU 2010. The lyophilized cheese is characterized by a comparatively low AI - 2.59 after lyophilization and is preserved during storage - 2.55. After lyophilization the LPS of the cheese was the highest – 103.70, while after irradiation a decrease of its value was observed up to 97.73 with 2 kGy and 96.91 with 4 kGy. Key words: white brine cheese by buffalo milk, atherogenic index, lipid preventive score, freeze-drying - lyophilization, gamma sterilization

  1. Fatty Acid Uptake and Lipid Storage Induced by HIF-1α Contribute to Cell Growth and Survival after Hypoxia-Reoxygenation

    Directory of Open Access Journals (Sweden)

    Karim Bensaad

    2014-10-01

    Full Text Available Summary: An in vivo model of antiangiogenic therapy allowed us to identify genes upregulated by bevacizumab treatment, including Fatty Acid Binding Protein 3 (FABP3 and FABP7, both of which are involved in fatty acid uptake. In vitro, both were induced by hypoxia in a hypoxia-inducible factor-1α (HIF-1α-dependent manner. There was a significant lipid droplet (LD accumulation in hypoxia that was time and O2 concentration dependent. Knockdown of endogenous expression of FABP3, FABP7, or Adipophilin (an essential LD structural component significantly impaired LD formation under hypoxia. We showed that LD accumulation is due to FABP3/7-dependent fatty acid uptake while de novo fatty acid synthesis is repressed in hypoxia. We also showed that ATP production occurs via β-oxidation or glycogen degradation in a cell-type-dependent manner in hypoxia-reoxygenation. Finally, inhibition of lipid storage reduced protection against reactive oxygen species toxicity, decreased the survival of cells subjected to hypoxia-reoxygenation in vitro, and strongly impaired tumorigenesis in vivo. : Bensaad et al. now show that FABP3 and FABP7 are induced by HIF-1α and lead to a significant lipid droplet (LD accumulation in hypoxia. In hypoxia-reoxygenation, ATP production occurs via fatty acid β-oxidation or glycogen degradation in a cell-type-dependent manner, while inhibition of LD formation increases ROS toxicity and decreases cell survival in vitro and strongly impairs tumorigenesis in vivo.

  2. Intensity of lipid oxidation and formation of cholesterol oxidation products during frozen storage of raw and cooked chicken

    OpenAIRE

    Conchillo, A. (Ana); Ansorena, D. (Diana); Astiasarán, I. (Iciar)

    2004-01-01

    Raw and cooked chicken breasts were stored at −18 °C for 3 months under aerobic and vacuum conditions, and the intensity of lipid oxidation and the formation of COP (cholesterol oxidation products) were studied. Raw samples showed low COP levels (4.60–7.40 µg g−1 fat), TBARS (thiobarbituric acid reactive substances) levels (0.01–0.03 mg kg−1) and peroxide values (not detected) under both aerobic and vacuum conditions. Cooked samples (grilled and roasted) showed TBARS levels of 0.36–0.99 mg kg...

  3. Armazenamento de sementes de Myracrodruon urundeuva Fr. All. em diferentes embalagens e ambientes Storage of Myracrodruon urundeuva Fr. All. seeds in different packaging and environments

    Directory of Open Access Journals (Sweden)

    R.S. Guedes

    2012-01-01

    Full Text Available Myracrodruon urundeuva Fr. All. é uma espécie pertencente à família Anacardiaceae, cuja planta pode ser utilizada como medicinal, na indústria de curtimento de couro, na arborização de ruas e praças, produzindo madeira de grande resistência mecânica. Diante disso, o objetivo deste trabalho foi determinar as melhores condições e períodos de armazenamento para conservação da viabilidade e vigor das sementes de M. urundeuva. As embalagens utilizadas para acondicionamento das sementes foram sacos de papel Kraft, algodão e de polietileno transparente, bem como folhas de papel alumínio. Em seguida as sementes foram armazenadas em ambiente natural de laboratório (25 ± 2ºC, freezer (-20 ± 2ºC, câmara fria (8 ± 2ºC e geladeira (6 ± 2ºC. Em intervalos pré-determinados (0, 30, 60, 90, 120, 150, 180, 210 e 240 dias avaliou-se o teor de água das sementes, porcentagem de emergência, índice de velocidade de emergência, comprimento e massa seca de plântulas. No ambiente de laboratório não houve conservação do vigor das sementes de M. urundeuva. A melhor condição para conservação de sementes de M. urundeuva foi obtida com o acondicionamento em sacos de papel Kraft, pano de algodão, plástico ou papel alumínio e manutenção em geladeira ou freezer, podendo também ser conservadas embaladas em papel ou alumínio, quando estocadas em câmara fria, por 240 dias. Alteração no vigor de sementes dessa espécie é primeiramente identificada pela redução da velocidade de emergência.Myracrodruon urundeuva Fr. All. is a species belonging to the Anacardiaceae family, which can be utilized as a medicinal plant, or in the leather tanning industry, urban afforestation, and the production of wood with great mechanic resistance. The objective of this study was to determine the best conditions and storage periods for preserving the viability and vigor of M. urundeuva. Seeds conditioned in bags of Kraft paper, cotton, polyethylene

  4. Analysis of factors affecting volatile compound formation in roasted pumpkin seeds with selected ion flow tube-mass spectrometry (SIFT-MS) and sensory analysis.

    Science.gov (United States)

    Bowman, T; Barringer, S

    2012-01-01

    Pumpkin (Cucurbita pepo and maxima) seeds are uniquely flavored and commonly consumed as a healthy roasted snack. The objective was to determine dominant volatiles in raw and roasted pumpkin seeds, and the effect of seed coat, moisture content, fatty acid ratio, total lipids, reducing sugars, and harvest year on volatile formation. Sensory was conducted to evaluate overall liking of seed variety and texture. Seed processing included extraction from the fruit, dehydration, and roasting (150 °C). Oil extraction was done using soxhlet, fatty acid profile using Gas Chromatography Flame Ionization Detector, and reducing sugars using 3,5-dinitrosalicylic acid and UV-spectroscopy. Headspace analysis of seeds was performed by selected ion flow tube-mass spectrometry (SIFT-MS). Volatiles dominating in raw pumpkin seeds were lipid aldehydes, ethyl acetate, 2,3-butandione, and dimethylsulfide. Compounds contributing to roasted aroma include alkylpyrazines and Strecker and lipid aldehydes. Overall, hull-less seeds had higher volatile lipid aldehydes and Strecker aldehydes. Seeds dehydrated to a moisture content of 6.5% before roasting had higher initial and final volatile concentrations than seeds starting at 50% moisture. Higher oil content resulted in higher lipid aldehyde formation during roasting with a moderate correlation between free fatty acid ratio and corresponding lipid aldehyde. Harvest year (2009 compared with 2010) had a significant impact on volatile formation in hull-less seeds, but not as much as variety differences. No significant correlation was found between reducing sugars and volatile formation. Sensory showed that hull-less seeds were liked significantly more than hulled seeds. Elucidation of aromatic flavor development during roasting with SIFT-MS provides information on flavor release and offers better control during processing. Knowledge of volatiles in raw and roasted pumpkin seeds and effects of seed coat, moisture content, seed composition, and

  5. Evaluation of food grade solvents for lipid extraction and impact of storage temperature on fatty acid composition of edible seaweeds Laminaria digitata (Phaeophyceae) and Palmaria palmata (Rhodophyta).

    Science.gov (United States)

    Schmid, Matthias; Guihéneuf, Freddy; Stengel, Dagmar B

    2016-10-01

    This study evaluated the impact of different food- and non-food grade extraction solvents on yield and fatty acid composition of the lipid extracts of two seaweed species (Palmaria palmata and Laminaria digitata). The application of chloroform/methanol and three different food grade solvents (ethanol, hexane, ethanol/hexane) revealed significant differences in both, extraction yield and fatty acid composition. The extraction efficiency, in terms of yields of total fatty acids (TFA), was in the order: chloroform/methanol>ethanol>hexane>ethanol/hexane for both species. Highest levels of polyunsaturated fatty acids (PUFA) were achieved by the extraction with ethanol. Additionally the effect of storage temperature on the stability of PUFA in ground and freeze-dried seaweed biomass was investigated. Seaweed samples were stored for a total duration of 22months at three different temperatures (-20°C, 4°C and 20°C). Levels of TFA and PUFA were only stable after storage at -20°C for the two seaweed species. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Proteomic dissection of seed germination and seedling establishment in Brassica napus

    Directory of Open Access Journals (Sweden)

    Jianwei Gu

    2016-10-01

    Full Text Available The success of seed germination and the establishment of a normal seedling are key determinants of plant species propagation. At present, only few studies have focused on the genetic control of the seed germination by proteomic approach in Brassica napus. In the present study, the protein expression pattern of seed germination was investigated using differential fluorescence two-dimensional gel electrophoresis (2-D DIGE in B. napus. One hundred thirteen differentially expressed proteins (DEPs, which were mainly involved in storage proteins (23.4%, energy metabolism (18.9%, protein metabolism (16.2%, defense/disease (12.6%, seed maturation (11.7%, carbohydrate metabolism (4.5%, lipid metabolism (4.5%, amino acids metabolism (3.6%, cell growth/division (3.6%, and some unclear proteins (2.7% were observed by proteomic analysis. Seventeen genes corresponding to 11 DEPs were identified within or near the associated linkage disequilibrium regions related to seed germination and vigor quantitative traits reported in B. napus in previous studies. The expression pattern of proteins showed the heterotrophic metabolism could be activated in the process of seed germination and the onset of defense system might start during seed germination. These findings will help us more in-depth understanding of the mobilization of seed storage reserves and regulation mechanisms of germination process in B. napus.

  7. Banking Wyoming big sagebrush seeds

    Science.gov (United States)

    Robert P. Karrfalt; Nancy Shaw

    2013-01-01

    Five commercially produced seed lots of Wyoming big sagebrush (Artemisia tridentata Nutt. var. wyomingensis (Beetle & Young) S.L. Welsh [Asteraceae]) were stored under various conditions for 5 y. Purity, moisture content as measured by equilibrium relative humidity, and storage temperature were all important factors to successful seed storage. Our results indicate...

  8. Study on seed storage protein of offspring 60Co γ-rays induced mutants of soybean Jindou-24

    International Nuclear Information System (INIS)

    Li Huifeng; Li Guiquan; Li Ling

    2008-01-01

    Seed of soybean variety Jindou-24 were irradiated with 60 Co γ-rays and the offspring of mutants were used to research the relative contents of glycinin (11S) and β-conglycinin (7S), the composition of its subunits, the contents of different subunits and the rules of its variance by using SDS-PAGE technique. the results indicated that the content of different subunits or the same subunit of different varieties varied greatly in the offspring of mutants. Significant difference existed in the contents of different subunits of the same variety or the same subunits of different varieties. The content of 11S negatively correlated with that of 7S, and positively correlated with 11S/7S. Cluster analysis showed the mutants were divided into three groups (average 11S/7S rate 1.70, 1.25 and 0.79) according to the rate of 11S/7S. The results could provide theoretical guidance to breed new soybean varieties with the character of high quality and other special purpose. (authors)

  9. The process of lipid storage in insect oocytes: The involvement of β-chain of ATP synthase in lipophorin-mediated lipid transfer in the chagas' disease vector Panstrongylus megistus (Hemiptera: Reduviidae).

    Science.gov (United States)

    Fruttero, Leonardo L; Leyria, Jimena; Ramos, Fabián O; Stariolo, Raúl; Settembrini, Beatriz P; Canavoso, Lilián E

    2017-01-01

    Lipophorin is the main lipoprotein in the hemolymph of insects. During vitellogenesis, lipophorin delivers its hydrophobic cargo to developing oocytes by its binding to non-endocytic receptors at the plasma membrane of the cells. In some species however, lipophorin may also be internalized to some extent, thus maximizing the storage of lipid resources in growing oocytes. The ectopic β chain of ATP synthase (β-ATPase) was recently described as a putative non-endocytic lipophorin receptor in the anterior midgut of the hematophagous insect Panstrongylus megistus. In the present work, females of this species at the vitellogenic stage of the reproductive cycle were employed to investigate the role of β-ATPase in the transfer of lipids to the ovarian tissue. Subcellular fractionation and western blot revealed the presence of β-ATPase in the microsomal membranes of the ovarian tissue, suggesting its localization in the plasma membrane. Immunofluorescence assays showed partial co-localization of β-ATPase and lipophorin in the membrane of oocytes as well as in the basal domain of the follicular epithelial cells. Ligand blotting and co-immunoprecipitation approaches confirmed the interaction between lipophorin and β-ATPase. In vivo experiments with an anti-β-ATPase antibody injected to block such an interaction demonstrated that the antibody significantly impaired the transfer of fatty acids from lipophorin to the oocyte. However, the endocytic pathway of lipophorin was not affected. On the other hand, partial inhibition of ATP synthase activity did not modify the transfer of lipids from lipophorin to oocytes. When the assays were performed at 4°C to diminish endocytosis, the results showed that the antibody interfered with lipophorin binding to the oocyte plasma membrane as well as with the transfer of fatty acids from the lipoprotein to the oocyte. The findings strongly support that β-ATPase plays a role as a docking lipophorin receptor at the ovary of P. megistus

  10. Global analysis of gene expression profiles in developing physic nut (Jatropha curcas L. seeds.

    Directory of Open Access Journals (Sweden)

    Huawu Jiang

    Full Text Available BACKGROUND: Physic nut (Jatropha curcas L. is an oilseed plant species with high potential utility as a biofuel. Furthermore, following recent sequencing of its genome and the availability of expressed sequence tag (EST libraries, it is a valuable model plant for studying carbon assimilation in endosperms of oilseed plants. There have been several transcriptomic analyses of developing physic nut seeds using ESTs, but they have provided limited information on the accumulation of stored resources in the seeds. METHODOLOGY/PRINCIPAL FINDINGS: We applied next-generation Illumina sequencing technology to analyze global gene expression profiles of developing physic nut seeds 14, 19, 25, 29, 35, 41, and 45 days after pollination (DAP. The acquired profiles reveal the key genes, and their expression timeframes, involved in major metabolic processes including: carbon flow, starch metabolism, and synthesis of storage lipids and proteins in the developing seeds. The main period of storage reserves synthesis in the seeds appears to be 29-41 DAP, and the fatty acid composition of the developing seeds is consistent with relative expression levels of different isoforms of acyl-ACP thioesterase and fatty acid desaturase genes. Several transcription factor genes whose expression coincides with storage reserve deposition correspond to those known to regulate the process in Arabidopsis. CONCLUSIONS/SIGNIFICANCE: The results will facilitate searches for genes that influence de novo lipid synthesis, accumulation and their regulatory networks in developing physic nut seeds, and other oil seeds. Thus, they will be helpful in attempts to modify these plants for efficient biofuel production.

  11. Changes during imbition of irradiated dry seeds of lettuce

    International Nuclear Information System (INIS)

    Smith, M.T.

    1981-01-01

    Since ionizing radiation has been shown to produce free-radicals in dry seeds, effect peroxidation of lipids in model systems, and cause changes in membrane permeability, it was decided to subject dry seeds of lettuce to gamma irradiation to determine whether the cytological changes which occurred on subsequent imbition are similar in any way to those occurring during natural ageing in dry storage. Seeds of 3% moisture content were radiated with gamma rays from a Cobalt 60 source at doses between 3,4 kGy and 23,8 kGy and the root tips prepared for TEM after 5 and 12 hours imbition. The cytological changes which occurred are shortly discussed

  12. Changes during imbition of irradiated dry seeds of lettuce

    Energy Technology Data Exchange (ETDEWEB)

    Smith, M T [Natal Univ., Durban (South Africa). Dept. of Biological Sciences

    1981-01-01

    Since ionizing radiation has been shown to produce free-radicals in dry seeds, effect peroxidation of lipids in model systems, and cause changes in membrane permeability, it was decided to subject dry seeds of lettuce to gamma irradiation to determine whether the cytological changes which occurred on subsequent imbition are similar in any way to those occurring during natural ageing in dry storage. Seeds of 3% moisture content were radiated with gamma rays from a Cobalt 60 source at doses between 3,4 kGy and 23,8 kGy and the root tips prepared for TEM after 5 and 12 hours imbition. The cytological changes which occurred are shortly discussed.

  13. Evaluation of the effects of Citrus sinensis seed oil on blood glucose, lipid profile and liver enzymes in rats injected with alloxan monohydrate

    Directory of Open Access Journals (Sweden)

    K.C Chilaka

    2015-06-01

    Conclusions: However, further studies need to be carried out to show its mechanism of action and to isolate the active ingredient in the Citrus sinensis seed oil that is responsible for these actions.

  14. Vigor of sunflower and soybean aging seed

    OpenAIRE

    Tatić M.; Balešević-Tubić S.; Ðorđević V.; Miklič V.; Vujaković M.; Ðukić V.

    2012-01-01

    Seed aging and deterioration affect seed vigor and viability. The characteristics of the chemical composition of oil crops seed are related to specific processes occurring in the seed during storage. This study was performed to examine the changes in seed vigor of different sunflower and soybean genotypes under controlled and conventional (uncontrolled) conditions of natural aging for six and twelve months. Obtained results show that the degree of seed dama...

  15. Seed coat darkening in Cowpea bean

    Science.gov (United States)

    Seed coat of cowpea bean (Vigna unguiculata L. Walp) slowly browns to a darker color during storage. High temperature and humidity during storage might contribute to this color change. Variation in browning rate among seeds in a lot leads to a mixture of seed colors creating an unacceptable product...

  16. Lipids, lipid droplets and lipoproteins in their cellular context; an ultrastructural approach

    NARCIS (Netherlands)

    Mesman, R.J.

    2013-01-01

    Lipids are essential for cellular life, functioning either organized as bilayer membranes to compartmentalize cellular processes, as signaling molecules or as metabolic energy storage. Our current knowledge on lipid organization and cellular lipid homeostasis is mainly based on biochemical data.

  17. Plant seeds as sources of potential industrial chemicals, pharmaceuticals, and pest control agents.

    Science.gov (United States)

    Powell, Richard G

    2009-03-27

    Investigations of natural products isolated from seeds have resulted in a remarkable variety of compounds having unusual structures. Seeds of many plant species contain uncommon fatty acids and lipids, some of which have found uses in the cosmetic industry or as renewable (non-petroleum based) industrial raw materials. In addition to proteins and energy storage substances such as carbohydrates and lipids, seeds generally contain, or have the ability to produce, protective compounds that are active as plant growth regulators, fungicides, insecticides, and repellents of herbivores; seeds occasionally contain compounds that are toxic to most other organisms. These compounds may also be present in other plant parts, but often are found at higher concentrations in seeds. Other compounds of interest have been associated with plant-endophyte interactions that are of mutual benefit to both organisms. Tests of seed extracts for cytotoxic and antitumor activity, toxicity to insects, and relationships to several animal disease syndromes have been revealing. Examples of compounds isolated from plant seeds that have served as lead compounds for additional research, or that continue to be of interest to researchers in multiple areas, are reviewed.

  18. A Heuristic T-S Fuzzy Model for the Pumped-Storage Generator-Motor Using Variable-Length Tree-Seed Algorithm-Based Competitive Agglomeration

    Directory of Open Access Journals (Sweden)

    Jianzhong Zhou

    2018-04-01

    Full Text Available With the fast development of artificial intelligence techniques, data-driven modeling approaches are becoming hotspots in both academic research and engineering practice. This paper proposes a novel data-driven T-S fuzzy model to precisely describe the complicated dynamic behaviors of pumped storage generator motor (PSGM. In premise fuzzy partition of the proposed T-S fuzzy model, a novel variable-length tree-seed algorithm based competitive agglomeration (VTSA-CA algorithm is presented to determine the optimal number of clusters automatically and improve the fuzzy clustering performances. Besides, in order to promote modeling accuracy of PSGM, the input and output formats in the T-S fuzzy model are selected by an economical parameter controlled auto-regressive (CAR model derived from a high-order transfer function of PSGM considering the distributed components in the water diversion system of the power plant. The effectiveness and superiority of the T-S fuzzy model for PSGM under different working conditions are validated by performing comparative studies with both practical data and the conventional mechanistic model.

  19. Novel missense mutations in PNPLA2 causing late onset and clinical heterogeneity of neutral lipid storage disease with myopathy in three siblings.

    Science.gov (United States)

    Missaglia, Sara; Tasca, Elisabetta; Angelini, Corrado; Moro, Laura; Tavian, Daniela

    2015-01-01

    Neutral lipid storage disease with myopathy (NLSD-M) is a rare autosomal recessive disorder characterised by an abnormal accumulation of triacylglycerol into cytoplasmic lipid droplets (LDs). NLSD-M patients are mainly affected by progressive myopathy, cardiomyopathy and hepatomegaly. Mutations in the PNPLA2 gene cause variable phenotypes of NLSD-M. PNPLA2 codes for adipose triglyceride lipase (ATGL), an enzyme that hydrolyses fatty acids from triacylglycerol. This report outlines the clinical and genetic findings in a NLSD-M Italian family with three affected members. In our patients, we identified two novel PNPLA2 missense mutations (p.L56R and p.I193F). Functional data analysis demonstrated that these mutations caused the production of ATGL proteins able to bind to LDs, but with decreased lipase activity. The oldest brother, at the age of 38, had weakness and atrophy of the right upper arm and kyphosis. Now he is 61 years old and is unable to raise arms in the horizontal position. The second brother, from the age of 44, had exercise intolerance, cramps and pain in lower limbs. He is currently 50 years old and has an asymmetric distal amyotrophy. One of the two sisters, 58 years old, presents the same PNPLA2 mutations, but she is still oligo-symptomatic on neuromuscular examination with slight triceps muscle involvement. She suffered from diabetes and liver steatosis. This NLSD-M family shows a wide range of intra-familial phenotypic variability in subjects carrying the same mutations, both in terms of target-organs and in terms of rate of disease progression. Copyright © 2015. Published by Elsevier Inc.

  20. Metabolic consequences of adipose triglyceride lipase deficiency in humans: an in vivo study in patients with neutral lipid storage disease with myopathy.

    Science.gov (United States)

    Natali, Andrea; Gastaldelli, Amalia; Camastra, Stefania; Baldi, Simona; Quagliarini, Fabiana; Minicocci, Ilenia; Bruno, Claudio; Pennisi, Elena; Arca, Marcello

    2013-09-01

    The role of adipose triglyceride lipase (ATGL) in intermediate substrates metabolism has not been fully elucidated in humans. Our objective was to evaluate the consequences of ATGL deficiency on body fat distribution, insulin sensitivity, fatty acids metabolism, and energy substrate utilization. Body composition and organ fat content were measured by bioimpedance and (1)H nuclear magnetic resonance spectroscopy; heart glucose metabolism by [(18)F]deoxyglucose positron emission tomography and insulin sensitivity and β-cell function by oral glucose tolerance and 2-step euglycemic-hyperinsulinemic clamp. Lipolysis ([(2)H5]glycerol turnover) and indirect calorimetry were evaluated at fasting, after oral glucose load, during the clamp, and also during an iv epinephrine infusion. These metabolic investigations were carried out during hospitalization. Three patients affected by neutral lipid storage disease with myopathy (NLSDM) due to homozygosity for loss-of-function mutations in the ATGL gene and 6 sex-, age-, and body mass index-matched controls were studied. As expected, NLSDM patients showed diffuse, although heterogeneous, fat infiltration in skeletal muscles associated with increased visceral fat. Although heart and liver were variably affected, fat content in the pancreas was increased in all patients. Compared with healthy controls, NLSDM patients showed impaired insulin response to glucose possibly related to the severe pancreatic steatosis, preserved whole-body insulin sensitivity, and a shift toward glucose metabolism in the heart. Fasting nonesterified fatty acid concentrations as well as basal lipolytic rates and the antilipolytic effect of insulin were normal in NLSDM patients, whereas the lipolytic effect of norepinephrine was impaired. Finally, no significant abnormality in the respiratory quotient was noted in NLSDM patients. In humans, ATGL has a remarkable effect on cellular lipid droplet handling, and its lack causes both perivisceral, skeletal

  1. Preparation and Low Temperature Short-term Storage for Synthetic Seeds of Caladium bicolor

    Directory of Open Access Journals (Sweden)

    Mehpara MAQSOOD

    2015-03-01

    Full Text Available An efficient somatic embryo encapsulation and in vitro plant regeneration technique were established with Caladium bicolor, an important ornamental plant.Tuber derived embryogenic callus (95.50% was obtained on Murashige and Skoog (MS medium amended with 0.5 mg L-1 α-Naphthalene acetic acid (NAA + 0.5 mg L-1 6-Benzyladenine (BA. The embryogenic callus later differentiated into somatic embryos in the same plant growth regulators (PGRs added medium (NAA and BA. The induced embryos matured and developed into plantlets in NAA and BA added media; maximum plantlets development was observed at 1.0 mg L-1 NAA + 1.0 mg L-1 BA supplemented medium. Synthetic seeds were produced by encapsulating embryos in gel containing 3.0% sucrose + 3.0% sodium alginate and 100 mM of calcium chloride.The highest synthetic seed germination (97.6% was observed on medium supplemented with 1.0 mg L-1 NAA + 1.0 mg L-1 BA. The synthetic seeds were kept at low temperatures for storage; the encapsulated beads were viable and demonstrated good germination even after 12 weeks of storage at 4 °C. The plantlet recovery frequency was however declined with time. The synthetic seed derived plantlets were morphologically similar to the mother plant.

  2. Germinação de sementes de porta-enxertos de citros após o armazenamento em ambiente refrigerado Seed germination of citrus rootstocks after cooling conditions storage

    Directory of Open Access Journals (Sweden)

    DALMO LOPES DE SIQUEIRA

    2002-08-01

    Full Text Available Este trabalho teve como objetivo avaliar o período de viabilidade das sementes dos porta-enxertos limoeiros-'Cravo' e 'Volkameriano', tangerineira-'Cleópatra' e citrumeleiro-'Swingle' armazenadas em embalagem permeável sob temperatura controlada de 5-7ºC por 150 dias. A cada 30 dias, foram avaliadas a germinação em laboratório e a emergência das plântulas em casa de vegetação. Determinou-se o grau de umidade das sementes em cada época de testes. O armazenamento nas condições citadas induziu menor velocidade de germinação nas sementes de 'Cravo' e 'Volkameriano', as quais não tiveram a germinação prejudicada quando apresentavam umidade próxima a 5%. Houve correlação altamente significativa entre teor de água e germinação nas sementes de 'Cleópatra' e 'Swingle'. Para a manutenção da viabilidade das sementes destes porta-enxertos, deve-se procurar manter o seu grau de umidade em torno de 20%. As sementes dos limoeiros-'Cravo' e 'Volkameriano' apresentaram maior velocidade de germinação e emergência que os demais porta-enxertos, enquanto as da tangerineira-'Cleópatra' apresentaram menor velocidade de emergência. Sementes de citrumeleiro- 'Swingle' e tangerineira-'Cleópatra' não germinaram após armazenamento por 60 e 90 dias, respectivamente.The present work was carried out in order to verify the viability period of the rootstocks 'Rangpur' lime, 'Volkameriano' lemon, 'Cleópatra' mandarin and 'Swingle' citrumelo seeds stored in permeable package at 5-7ºC for 150 days. The Germination in laboratory, seedling emergence in greenhouse and seed moisture content were performed each 30 days. Seed storage in that conditions reduced the speed of emergence index in the 'Rangpur' and 'Volkameriano' seeds. The germination of these rootstocks seeds weren't reduced when the seed moisture content was near 5%. There was highly significative correlation between seed water content and germination porcentage of 'Cleópatra' and

  3. Efficacy of Some Essential Oils Against Aspergillus flavus with Special Reference to Lippia alba Oil an Inhibitor of Fungal Proliferation and Aflatoxin B1 Production in Green Gram Seeds during Storage.

    Science.gov (United States)

    Pandey, Abhay K; Sonker, Nivedita; Singh, Pooja

    2016-04-01

    During mycofloral analysis of green gram (Vigna radiata (L.) R. Wilczek) seed samples taken from different grocery stores by agar and standard blotter paper methods, 5 fungal species were identified, of which Aspergillus flavus exhibited higher relative frequency (75.20% to 80.60%) and was found to produce aflatoxin B1 . On screening of 11 plant essential oils against this mycotoxigenic fungi, Lippia alba essential oil was found to be most effective and showed absolute inhibition of mycelia growth at 0.28 μL/mL. The oil of L. alba was fungistatic and fungicidal at 0.14 and 0.28 μL/mL, respectively. Oil had broad range of fungitoxicity at its MIC value and was absolutely inhibited the AFB1 production level at 2.0 μL/mL. Chemical analysis of this oil revealed geranial (36.9%) and neral (29.3%) as major components followed by myrcene (18.6%). Application of a dose of 80 μL/0.25 L air of Lippia oil in the storage system significantly inhibited the fungal proliferation and aflatoxin production without affecting the seed germination rate. By the virtue of fungicidal, antiaflatoxigenic nature and potent efficacy in storage food system, L. alba oil can be commercialized as botanical fungicide for the protection of green gram seeds during storage. © 2016 Institute of Food Technologists®

  4. The effects of feed composition on the sensory quality of organic rainbow trout during ice storage

    DEFF Research Database (Denmark)

    Green-Pedersen, Ditte; Hyldig, Grethe; Jacobsen, Charlotte

    fishmeal and a mixture of protein from organic vegetable, while the lipid sources were fish oil and organic oil from linseed, sunflower, rapeseed and grape seed. Sensory analysis was performed after 3, 5, 7 and 14 days of storage in ice. The results showed that both protein and lipid source in the feed can...... after 14 days of storage, indicating that vegetable protein in the feed increases the self-life of organic rainbow trout.......The focus of this work was to study which effects the type of protein and lipid source in the feed for organic Rainbow trout influences had on the sensory quality of final product. Two and four different protein and lipid sources were used in the experiment respectively. The protein sources were...

  5. Late onset of neutral lipid storage disease due to novel PNPLA2 mutations causing total loss of lipase activity in a patient with myopathy and slight cardiac involvement.

    Science.gov (United States)

    Missaglia, Sara; Maggi, Lorenzo; Mora, Marina; Gibertini, Sara; Blasevich, Flavia; Agostoni, Piergiuseppe; Moro, Laura; Cassandrini, Denise; Santorelli, Filippo Maria; Gerevini, Simonetta; Tavian, Daniela

    2017-05-01

    Neutral lipid storage disease with myopathy (NLSDM) presents with skeletal muscle myopathy and severe dilated cardiomyopathy in nearly 40% of cases. NLSDM is caused by mutations in the PNPLA2 gene, which encodes the adipose triglyceride lipase (ATGL). Here we report clinical and genetic findings of a patient carrying two novel PNPLA2 mutations (c.696+4A>G and c.553_565delGTCCCCCTTCTCG). She presented at age 39 with right upper limb abduction weakness slowly progressing over the years with asymmetric involvement of proximal upper and lower limb muscles. Cardiological evaluation through ECG and heart echo scan was normal until the age 53, when mild left ventricular diastolic dysfunction was detected. Molecular analysis revealed that only one type of PNPLA2 transcript, with exon 5 skipping, was expressed in patient cells. Such aberrant mRNA causes the production of a shorter ATGL protein, lacking part of the catalytic domain. This is an intriguing case, displaying severe PNPLA2 mutations with clinical presentation characterized by slight cardiac impairment and full expression of severe asymmetric myopathy. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  6. Qualidade fisiológica de sementes de palmiteiro-vermelho em função da desidratação e do armazenamento Physiological quality of Euterpe espiritosantensis seeds as affected by partial drying and storage

    Directory of Open Access Journals (Sweden)

    Cibele C Martins

    2007-06-01

    recalcitrant, presenting sensibility to dehydration and difficulty for storage. In some species with recalcitrant seeds, partial drying favors the maintenance of viability during storage. The objective of this research was to study the effects of partial drying and storage on germination and vigor of E. espiritosantensis seeds. Two seed lots (L1 and L2 were dried for 0; 20, and 40 hours in a dry chamber and stored at 15ºC in closed plastic bags. An assay for each seed lot was carried out on a completely randomized design, with treatments arranged in an 3 x 6 (L1 and 3 x 10 (L2 factorial scheme, corresponding to three seed drying periods and six or ten weeks of storage. Seed quality was evaluated every six weeks, during 30 and 54 weeks for L1 and L2, respectively. Seed water content (U, percentage of germination (G and vigor (first counting of germination test and index germination rate were assessed. Water content during storage, were, in both lots, on average 46; 40, and 36%, respectively, for 0; 20, and 40 hours of drying, without important changes due to storage period. For non-stored seeds, drying reduced significantly vigor, but did not interfere with germination. Seed lot L1 presented viable seeds in all sub lots until the end of the storage period (G = 71, 61 and 45% for 0; 20 and 40 hours, respectively. Seeds on lot L2 lost viability completely in the 30th week in the 40 hours of drying treatment (U=36%. Seeds in other treatments remained viable up to 54 weeks of storage (G = 46%. Partial drying reduced vigor and decreased seed longevity. Seeds from E. espiritosantensis can be stored without drying, with 45-46% of water content.

  7. Enzyme activity and reserve mobilization during Macaw palm ( Acrocomia aculeata seed germination

    Directory of Open Access Journals (Sweden)

    Elisa Monteze Bicalho

    2016-01-01

    Full Text Available ABSTRACT Reserve mobilization in seeds occurs after visible germination, which is marked by the protrusion of the radicle or cotyledonary petiole, as in species of Arecaceae. Acrocomia aculeata (macaw palm, usually produces hard seeds whose endosperm has mannan-rich cell walls. We investigated the composition of storage compounds in macaw palm seed and the roles of two enzymes (endo-β-mannanase, α-galactosidase during and after germination. The seeds were firstly submitted to pre-established protocol to overcome dormancy and promote germination. Enzyme activity in both embryo and endosperm were assayed from the initiation of germinative activities until leaf sheath appearance, and the status of seed structures and reserve compounds were evaluated. Protein content of the embryo decreased with the initiation of imbibition while the lipid content began decreasing six days after removal of the operculum. Increases in enzyme activity and starch content were both observed after visible germination. We suggest that endo-β-mannanase and α-galactosidase become active immediately at germination, facilitating haustorium expansion and providing carbohydrates for initial seedling development. Protein is the first storage compound mobilized during early imbibition, and the observed increase in the starch content of the haustorium was related to lipid degradation in that organ and mannan degradation in the adjacent endosperm.

  8. Gamma Radiation Impact on the Survival Microflora and Biochemical Constituents of Stored Anise Seeds (Pimpenella anisum L.)

    International Nuclear Information System (INIS)

    Mohamed, G.A.; Botros, H.W.; Ahmed, B.M.; Abo-El-Seoud, M.A.

    2010-01-01

    Anise seeds (Pimpenella anisum L.) were exposed to gamma irradiation doses (0, 4, 8, 16 and 32 KGy). Irradiated and un-irradiated anise seeds were stored for 3, 6 and 12 months and tested for their microbial population . In addition, the chemical constituents (volatile oils, fatty acids, lipids and sugars) were evaluated. The predominant microorganisms contaminated anise seeds include different fungal species identified as Aspergillus niger, A. ochraceus, A. parasiticus, A. flavus, A. fumigatus , Penicillium funiculosum , P. italicum , Rhizopus sp. and Trichoderma viride. Different bacterial species as Bacillus circulans, B. subtilis, B. megaterium, B. brevis, Pseudomonas fluorescens, P. oleourans and Alicyclobacillus acidocaldarius were recorded. The imposed irradiation doses showed a promising effect to decontaminate the microbial load , which in turn showed prolong the storage periods. Volatile oil content was relatively declined during storage. In addition, some alternations were happened regarding the essential oils constituents. However, the applied irradiation doses maintained the volatile oil content and retained its constituents near to the normal control. Total lipids content were not influenced by either the applied storage periods or the used gamma irradiation doses. However, the fatty acids methyl ester showed some changes due to the imposed treatments. Sugars content were increased in the stored and un-irradiated seeds, however, the exposure to gamma radiation reduced this increase and retained sugars content near to the normal level of the control seeds.

  9. Evaluation of the physiological quality of anadenenthera peregrina (L. Speg. seeds during storage Avaliação da qualidade fisiológica de sementes de Anadenanthera peregrina (L. Speg. durante o armazenamento

    Directory of Open Access Journals (Sweden)

    Daniel Santos Pinho

    2009-02-01

    Full Text Available The aim of this experiment was to evaluate the effect of storage temperature on the viability and vigour of A. peregrina seeds. Seeds of A. peregrina harvested in Viçosa-MG, in September 2005 were used. The seeds were stored in the Laboratory of Forest Seeds Analysis (LASF of the Universidade Federal de Viçosa (UFV, in a cold chamber, under approximately 5ºC over150 days. The seeds were stored under 20ºC for 150 days more. Seed moisture was determined after oven-drying at 105 ± 3ºC for 24 hours. The seeds were analyzed monthly using germination, tetrazolium and electrical conductivity tests and Osmopriming during 300 days, with four repetitions of 25 seeds. A. peregrina seeds kept a constant percentage of germination and viable seeds under the tetrazolium test for five months of storage, under either 5ºC and 20ºC. The electrical conductivity test showed significant differences in relation to the storage period, proving to be more sensitive than the standard germination test. Seed priming in PEG -0,4 MPa solution was not efficient in recovering the viability and vigor of the stored seeds at 5ºC and 20ºC.Este trabalho teve como objetivo avaliar o efeito da temperatura de armazenamento na viabilidade e vigor de sementes de angico (A. peregrina. As sementes utilizadas neste estudo foram colhidas na região de Viçosa, MG, em setembro de 2005, e armazenadas em câmara fria no Laboratório de Análise de Sementes Florestais (LASF da Universidade Federal de Viçosa (UFV por 150 dias, em uma temperatura de aproximadamente 5 ºC. A partir daí, as sementes foram armazenadas a aproximadamente a 20 ºC por mais 150 dias. O teor de água das sementes foi determinado após a secagem em estufa a 105 ± 3 ºC por 24 h. As sementes foram analisadas, mensalmente, pelo teste de germinação, teste de tetrazólio, teste de condutividade elétrica e osmocondicionamento, durante os 300 dias de armazenamento e com quatro repetições de 25 sementes. As

  10. Soybean seed viability and changes of fatty acids content as ...

    African Journals Online (AJOL)

    The characteristics of soybean seed chemical composition are related to specific processes occurring in seed during storage. These changes lead to seed aging during storage and affect seed vigour and content of fatty acids. In order to reveal severity of their influence, the following vigour tests were applied: Standard ...

  11. Different effects of diets rich in olive oil, rapeseed oil and sunflower-seed oil on postprandial lipid and lipoprotein concentrations and on lipoprotein oxidation susceptibility

    DEFF Research Database (Denmark)

    Nielsen, Nina Skall; Pedersen, A.; Sandstrøm, B.

    2002-01-01

    oxidation of fasting and postprandial lipoproteins eighteen males consumed diets enriched with rapeseed oil (RO), olive oil (OO), or sunflower-seed oil (SO) in randomised order for periods of 3 weeks followed by a RO test meal. In the postprandial state the concentrations of cholesterol and triacylglycerol...

  12. Some physicochemical characteristics of pinus (Pinus halepensis Mill., Pinus pinea L., Pinus pinaster and Pinus canariensis) seeds from North Algeria, their lipid profiles and volatile contents.

    Science.gov (United States)

    Kadri, Nabil; Khettal, Bachra; Aid, Yasmine; Kherfellah, Souraya; Sobhi, Widad; Barragan-Montero, Veronique

    2015-12-01

    Physicochemical characteristics of seeds of some pinus species (Pinus halepensis Mill., Pinus pinea L., Pinus pinaster and Pinus canariensis) grown in North Algeria were determined. The results showed that the seeds consist of 19.8-36.7% oil, 14.25-26.62% protein, 7.8-8.6% moisture. Phosphorus, potassium and magnesium were the predominant elements present in seeds. Pinus seed's oil physicochemical properties show acid values (4.9-68.9), iodine values (93.3-160.4) and saponification values (65.9-117.9). Oil analysis showed that the major unsaturated fatty acids for the four species were linoleic acid (30-59%) and oleic acid (17.4-34.6%), while the main saturated fatty acid was palmitic acid (5-29%). Gas Chromatography and Mass Spectrometry analysis of P. halepensis Mill., P. pinaster and P. canariensis volatile oils indicated that the major volatile compound was the limonene with relative percentage of 3.1, 7.5 and 10.8, respectively. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. Encapsulated phosphates reduce lipid oxidation in both ground chicken and ground beef during raw and cooked meat storage with some influence on color, pH, and cooking loss.

    Science.gov (United States)

    Kılıç, B; Simşek, A; Claus, J R; Atılgan, E

    2014-05-01

    Effects of encapsulated sodium tripolyphosphate (STP), sodium hexametaphosphate (HMP) and sodium pyrophosphate (SPP) on lipid oxidation in uncooked (0, 2, 24h) and cooked (0, 1, 7 d) ground chicken and beef during storage were determined. Ten phosphate treatments included a control (no phosphate), three unencapsulated (u) at 0.5% and three encapsulated (e) phosphates (0.5%) each at a low (e-low) and high (e-high) coating level. Two heating rates (slow, fast) were investigated. Cooking loss (CL), pH, color, orthophosphate (OP), TBARS and lipid hydroperoxides (LPO) were determined. A fast heating and uSTP resulted in lower CL (pcooked samples. Not increased coating level but encapsulated phosphates decreased lipid oxidation in cooked samples (p<0.05). Copyright © 2014 Elsevier Ltd. All rights reserved.

  14. Physiological quality of vegetable soybean seeds produced with different fertilization and storage for twelve months = Potencial fisiológico de sementes de soja-hortaliça produzidas com diferentes adubações e armazenadas por doze meses

    Directory of Open Access Journals (Sweden)

    Juliana Maria Espíndola Lima

    2013-04-01

    Full Text Available Abstract - The vegetable soybean (Glycine max (L. Merrill has characteristics that permit utilization in food as a vegetablewhen the seeds are still immature (stage R6 and occupy 80-90% of the cavity filling the pod. The objective of this study was to evaluate the physiological potential of seeds of two vegetable soybean genotypes produced with different fertilization and stored for 12 months. In seed production field was used in the experimental design of randomized blocks with 5x2 factorial, five fertilization (conventional, intermediate, alternative, manipueira and rice hulls and two periods of evaluation (at 12 months of storage. Evaluations conducted under laboratory conditions included mass of a hundred seeds, vigor and physiological quality considering germination, germination first count, electrical conductivity, potassium leaching, sand emergence and emergence rate of seedlings. The seeds of both vegetable soybean genotype were stored in polyethylene terephthalate bottles for 12 months, as to assess physical and physiological quality under storage. Seeds of BR9452273 produced with carbonized ricehusk showed better quality at harvest and presented greater reduced of vigour after 12 months of storage. BRS 258 cultivar conventional fertilization showed seeds of less physiological quality and higher reduced the vigour after 12 months of storage. The two vegetable soybean cultivars have quality reduced during storage of seed of twelve months in bottles of polyethylene terephthalate. = Resumo – A soja-hortaliça (Glycine max (L. Merrill apresenta características que permitem utilização na alimentação humana como hortaliça, quando as sementes estão ainda imaturas (estádio R6 e ocupam 80 a 90% do preenchimento da cavidade da vagem. Objetivou-se com este trabalho avaliar o potencial fisiológico das sementes de dois genótipos de soja-hortaliça produzidas com diferentes adubações e armazenadas por 12 meses. O delineamento

  15. Viabilidade e vigor de sementes de paineira após armazenamento, condicionamento e estresses salino e térmico Viability and vigor in folk silk tree seeds after storage, conditioning and submission to salt and thermal stress

    Directory of Open Access Journals (Sweden)

    Sonia Cristina Juliano Gualtieri de Andrade Perez

    2005-06-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito do condicionamento e dos estresses salino e térmico sobre o vigor e a viabilidade de sementes de paineira (Chorisia speciosa St.-Hil armazenadas em geladeira, em embalagens impermeáveis, durante três anos. Sementes com e sem punção do tegumento foram condicionadas em água destilada e em soluções de KNO3 a 0,1 M e 0,2 M, durante 24 horas, a 27ºC. O estresse salino foi simulado com soluções de NaCl em potenciais osmóticos variando de 0,0 a -1,2 MPa, a 27ºC. O estresse térmico de diferentes intensidades foi aplicado em sementes secas, que germinaram a 27ºC. Houve diminuição da viabilidade e do vigor das sementes com o aumento da intensidade dos estresses salino e térmico. Nas sementes condicionadas e intactas, o limite máximo de tolerância à salinidade não foi reduzido, porém houve diminuição da tolerância ao estresse térmico, em sementes condicionadas com ou sem punção. O teste de condutividade elétrica indicou as sementes condicionadas em água e KNO3 0,1 M como as de maior vigor. Sementes condicionadas em água apresentaram maior velocidade de germinação sob estresses salino e térmico. O condicionamento substituiu a punção do tegumento, que não é recomendada para sementes armazenadas.The aim of this work was to evaluate the effects of priming, salt and thermal stress on viability and vigor of folk silk tree seeds (Chorisia speciosa St.-Hil after storage in hermetic containers at 5ºC. Intact and with coat punction seeds were primed in distilled water and KNO3 solutions (0.1 M and 0.2 M during 24 hours at 27ºC. Salt stress was simulated with NaCl solutions at different osmotic potential, ranging from 0.0 MPa until -1.2 MPa, at 27ºC. Different intensities of thermal stress were applied on dried seeds; germination tests were carried out at 27ºC. As the intensity of salt and thermal stress increased, a reduction on seed vigor and viability was recorded in intact

  16. Influence of storage period of gamma-irradiated seeds on the catalase activity and the sinthesis of alpha-amilase in maize

    International Nuclear Information System (INIS)

    Velichkov, D.; Antonov, M.; Spasov, S.

    1983-10-01

    It was established that irradiation of the maize hybrid Knezha 2L-611 seeds with the dose 5 Gy showed the most constant and the best expressed stimulation effect. The activity of catalase and synthesis of alpha-amylase showed that the most convenient time for sowing of seeds irradiated with 5 Gy was 2 days after the treatment

  17. Forest Seed Collection, Processing,and Testing

    DEFF Research Database (Denmark)

    Schmidt, Lars Holger

    2016-01-01

    This chapter pertains to the techniques of capturing the best genetic quality seeds a seed source can produce at the optimal time of high physiological maturity and maintaining these qualities throughout the handling processes, all at a minimum cost. Different collection and processing techniques...... apply to different species, seed types, situations, and purposes. Yet the collection and processing toolbox contains a number of “standard” methods for most of these groups. Records and documentation help in evaluating “best practice” for future method improvement, and it helps in linking offspring...... to seed source. Conditions are set for short- and long-term seed storage by their inert storability physiology. The potential storage life of seed may for some robust “orthodox” species be several decades, while no available storage conditions can maintain viability for sensitive “recalcitrant” seed. Seed...

  18. Genetic Basis of Variation in Rice Seed Storage Protein (Albumin, Globulin, Prolamin, and Glutelin) Content Revealed by Genome-Wide Association Analysis.

    Science.gov (United States)

    Chen, Pingli; Shen, Zhikang; Ming, Luchang; Li, Yibo; Dan, Wenhan; Lou, Guangming; Peng, Bo; Wu, Bian; Li, Yanhua; Zhao, Da; Gao, Guanjun; Zhang, Qinglu; Xiao, Jinghua; Li, Xianghua; Wang, Gongwei; He, Yuqing

    2018-01-01

    Rice seed storage protein (SSP) is an important source of nutrition and energy. Understanding the genetic basis of SSP content and mining favorable alleles that control it will be helpful for breeding new improved cultivars. An association analysis for SSP content was performed to identify underlying genes using 527 diverse Oryza sativa accessions grown in two environments. We identified more than 107 associations for five different traits, including the contents of albumin (Alb), globulin (Glo), prolamin (Pro), glutelin (Glu), and total SSP (Total). A total of 28 associations were located at previously reported QTLs or intervals. A lead SNP sf0709447538, associated for Glu content in the indica subpopulation in 2015, was further validated in near isogenic lines NIL(Zhenshan97) and NIL(Delong208), and the Glu phenotype had significantly difference between two NILs. The association region could be target for map-based cloning of the candidate genes. There were 13 associations in regions close to grain-quality-related genes; five lead single nucleotide polymorphisms (SNPs) were located less than 20 kb upstream from grain-quality-related genes ( PG5a , Wx , AGPS2a , RP6 , and, RM1 ). Several starch-metabolism-related genes ( AGPS2a , OsACS6 , PUL , GBSSII , and ISA2 ) were also associated with SSP content. We identified favorable alleles of functional candidate genes, such as RP6 , RM1 , Wx , and other four candidate genes by haplotype analysis and expression pattern. Genotypes of RP6 and RM1 with higher Pro were not identified in japonica and exhibited much higher expression levels in indica group. The lead SNP sf0601764762, repeatedly detected for Alb content in 2 years in the whole association population, was located in the Wx locus that controls the synthesis of amylose. And Alb content was significantly and negatively correlated with amylose content and the level of 2.3 kb Wx pre-mRNA examined in this study. The associations or candidate genes identified would

  19. Genetic Basis of Variation in Rice Seed Storage Protein (Albumin, Globulin, Prolamin, and Glutelin Content Revealed by Genome-Wide Association Analysis

    Directory of Open Access Journals (Sweden)

    Pingli Chen

    2018-05-01

    Full Text Available Rice seed storage protein (SSP is an important source of nutrition and energy. Understanding the genetic basis of SSP content and mining favorable alleles that control it will be helpful for breeding new improved cultivars. An association analysis for SSP content was performed to identify underlying genes using 527 diverse Oryza sativa accessions grown in two environments. We identified more than 107 associations for five different traits, including the contents of albumin (Alb, globulin (Glo, prolamin (Pro, glutelin (Glu, and total SSP (Total. A total of 28 associations were located at previously reported QTLs or intervals. A lead SNP sf0709447538, associated for Glu content in the indica subpopulation in 2015, was further validated in near isogenic lines NIL(Zhenshan97 and NIL(Delong208, and the Glu phenotype had significantly difference between two NILs. The association region could be target for map-based cloning of the candidate genes. There were 13 associations in regions close to grain-quality-related genes; five lead single nucleotide polymorphisms (SNPs were located less than 20 kb upstream from grain-quality-related genes (PG5a, Wx, AGPS2a, RP6, and, RM1. Several starch-metabolism-related genes (AGPS2a, OsACS6, PUL, GBSSII, and ISA2 were also associated with SSP content. We identified favorable alleles of functional candidate genes, such as RP6, RM1, Wx, and other four candidate genes by haplotype analysis and expression pattern. Genotypes of RP6 and RM1 with higher Pro were not identified in japonica and exhibited much higher expression levels in indica group. The lead SNP sf0601764762, repeatedly detected for Alb content in 2 years in the whole association population, was located in the Wx locus that controls the synthesis of amylose. And Alb content was significantly and negatively correlated with amylose content and the level of 2.3 kb Wx pre-mRNA examined in this study. The associations or candidate genes identified would provide

  20. (Heckel) seeds

    African Journals Online (AJOL)

    UTILISATEUR

    Garcinia kola seeds to six different hormonal pre-germination treatments. This consisted of ... Thus, seed dormancy in this case is not a coat- imposed .... development of the cultivation of the species. The cause .... Hormonal regulation of seed ...

  1. Quince tree (cydonia oblonga Mill.)-breeding bases:seed propagation, cytogenetics and radiosensitivity

    International Nuclear Information System (INIS)

    Dall'Orto, F.A.C.

    1982-01-01

    The following aspects of the marmeleiro, cydonia oblonga Mill., were, researched: media nad periods to supply the seed chilling requirement in moist cold storage (5-10 0 c); quince seeds viability prepared by several extraction processes; seed germination and seedling development; cytogenetic aspects; seeds viability influenced by storage conditions and periods of time for storage; preliminary determination of seed radiosensitivity; concentrations of some macro and micronutrients in quince seedlings obtained from irradiated seeds, and radiosensitivity and interphasic nuclear volumes. (MAC) [pt

  2. Qualidade fisiológica de sementes de Ocimum selloi benth: sob condições de luz, temperatura e tempo de armazenamento Physiological quality of Ocimum selloi Benth: seeds under conditions of light, temperature and storage time

    Directory of Open Access Journals (Sweden)

    Larissa Corrêa do Bomfim Costa

    2010-06-01

    Full Text Available O atroveran ou elixir-paregórico (Ocimum selloi Benth. é uma espécie aromática nativa do Brasil, produtora de óleo essencial rico em metil-chavicol. Neste trabalho, objetivou-se avaliar a influência da temperatura, luz e tempo de armazenamento em diferentes ambientes sobre a germinação e o vigor de sementes de O. selloi. Inicialmente, testou-se o efeito da presença e ausência de luz branca sob fotoperíodo de 12 horas para determinar o fotoblastismo das sementes em diferentes temperaturas (20, 25, 30 e 35ºC. Em seguida, testou-se a germinação e emergência no armazenamento sob duas temperaturas (ambiente e câmara fria aos 0, 2, 4, 6, 8 10 e 12 meses de armazenamento. A espécie foi classificada como fotoblástica neutra, apresentando germinação em ampla faixa de temperatura (20 a 30ºC. A germinação das sementes manteve-se por doze meses de armazenamento em câmara fria e à temperatura ambiente, entretanto, o vigor decresce a partir de seis meses em armazenamento sob temperatura ambiente."Atroveran" or "elixir-paregórico" (Ocimum selloi Benth. is a Brazilian species that produces an essential oil rich in methyl chavicol. This work aimed to evaluate the influence of light, temperature and time of storage in different environments on the germination and vigor of O. selloi seeds. We initially tested the influence of presence and absence of white light in a 12-hour photoperiod to determine the photoblastism of the seeds under different temperatures (20, 25, 30 and 35ºC. The physiological quality of seeds conserved under two temperatures (room temperature and cold chamber was tested at 0, 2, 4, 6, 8 10 and 12 months of storage. The species was classified as neutral photoblastic with germination at a wide temperature range (20ºC-30°C. Germination was maintained for twelve months of storage in cold chamber at room temperature, but the vigor was reduced in seeds kept at room temperature after the sixth month of storage.

  3. Estudos sôbre a conservaç��o de sementes: XII - Melancia The influence of humidity and temperature on viability of watermelon seeds in storage

    Directory of Open Access Journals (Sweden)

    Eduardo Zink

    1964-01-01

    Full Text Available Sementes de melancia (Citrullus vulgaris Schrad. dos cultivares «Charleston Gray» e «Yamato Satô» foram conservadas em ambientes que diferiam entre si na umidade e na temperatura. Os resultados dos testes de germinação mostraram que as sementes perderam o poder germinativo com rapidez, quando conservadas em ambientes de alta umidade e temperatura elevada, cêrca de 30°C. Queda menos pronunciada foi observada quando se baixou a temperatura para 20ºC. Reduzindo-se apenas a umidade do ar, as sementes conservaram muito bem o poder germinativo até o final do ensaio. Quando, porém, tais sementes foram mantidas em baixas condições de umidade e temperatura, a capacidade germinativa conservou-se inalterada, ao longo dos dezoito meses de observações.The viability of watermelon seeds kept under different storage conditions, was studied during a period of 18 months. The experimental results presented in this paper drew the following conclusions: 1 Complete viability at the end of 18 months was obtained for seeds stored at 20°C and at relative humidity of 45%. 2 At this same temperature, the longevity of the seeds decreased with the increasing of relative humidity. 3 Seeds kept at 30°C, even in low relative humidity condition, lost their viability in a short time. 4 The seeds of watermelon stored in airtight containers at room temperature, kept their viability well for 12 months, after which time it decreased considerably.

  4. Regulation of wheat seed dormancy by after-ripening is mediated by specific transcriptional switches that induce changes in seed hormone metabolism and signaling.

    Directory of Open Access Journals (Sweden)

    Aihua Liu

    Full Text Available Treatments that promote dormancy release are often correlated with changes in seed hormone content and/or sensitivity. To understand the molecular mechanisms underlying the role of after-ripening (seed dry storage in triggering hormone related changes and dormancy decay in wheat (Triticum aestivum, temporal expression patterns of genes related to abscisic acid (ABA, gibberellin (GA, jasmonate and indole acetic acid (IAA metabolism and signaling, and levels of the respective hormones were examined in dormant and after-ripened seeds in both dry and imbibed states. After-ripening mediated developmental switch from dormancy to germination appears to be associated with declines in seed sensitivity to ABA and IAA, which are mediated by transcriptional repressions of PROTEIN PHOSPHATASE 2C, SNF1-RELATED PROTEIN KINASE2, ABA INSENSITIVE5 and LIPID PHOSPHATE PHOSPHTASE2, and AUXIN RESPONSE FACTOR and RELATED TO UBIQUITIN1 genes. Transcriptomic analysis of wheat seed responsiveness to ABA suggests that ABA inhibits the germination of wheat seeds partly by repressing the transcription of genes related to chromatin assembly and cell wall modification, and activating that of GA catabolic genes. After-ripening induced seed dormancy decay in wheat is also associated with the modulation of seed IAA and jasmonate contents. Transcriptional control of members of the ALLENE OXIDE SYNTHASE, 3-KETOACYL COENZYME A THIOLASE, LIPOXYGENASE and 12-OXOPHYTODIENOATE REDUCTASE gene families appears to regulate seed jasmonate levels. Changes in the expression of GA biosynthesis genes, GA 20-OXIDASE and GA 3-OXIDASE, in response to after-ripening implicate this hormone in enhancing dormancy release and germination. These findings have important implications in the dissection of molecular mechanisms underlying regulation of seed dormancy in cereals.

  5. Skeletal Muscle Magnetic Resonance Imaging of the Lower Limbs in Late-onset Lipid Storage Myopathy with Electron Transfer Flavoprotein Dehydrogenase Gene Mutations

    Institute of Scientific and Technical Information of China (English)

    Xin-Yi Liu; Ming Jin; Zhi-Qiang Wang; Dan-Ni Wang; Jun-Jie He; Min-Ting Lin; Hong-Xia Fu

    2016-01-01

    Background:Lipid storage myopathy (LSM) is a genetically heterogeneous group with variable clinical phenotypes.Late-onset multiple acyl-coenzyme A dehydrogenation deficiency (MADD) is a rather common form of LSM in China.Diagnosis and clinical management of it remain challenging,especially without robust muscle biopsy result and genetic detection.As the noninvasion and convenience,muscle magnetic resonance imaging (MRI) is a helpful assistant,diagnostic tool for neuromuscular disorders.However,the disease-specific MRI patterns of muscle involved and its diagnostic value in late-onset MADD have not been systematic analyzed.Methods:We assessed the MRI pattern and fat infiltration degree of the lower limb muscles in 28 late-onset MADD patients,combined with detailed clinical features and gene spectrum.Fat infiltration degree of the thigh muscle was scored while that ofgluteus was described as obvious or not.Associated muscular atrophy was defined as obvious muscle bulk reduction.Results:The mean scores were significantly different among the anterior,medial,and posterior thigh muscle groups.The mean of fat infiltration scores on posterior thigh muscle group was significantly higher than either anterior or medial thigh muscle group (P < 0.001).Moreover,the mean score on medial thigh muscle group was significantly higher than that of anterior thigh muscle group (P < 0.01).About half of the patients displayed fat infiltration and atrophy in gluteus muscles.Of 28 patients,12 exhibited atrophy in medial and/or posterior thigh muscle groups,especially in posterior thigh muscle group.Muscle edema pattern was not found in all the patients.Conclusions:Late-onset MADD patients show a typical muscular imaging pattern of fat infiltration and atrophy on anterior,posterior,and medial thigh muscle groups,with major involvement of posterior thigh muscle group and gluteus muscles and a sparing involvement of anterior thigh compartment.Our findings also suggest that muscle MRI of

  6. Qualidade fisiológica de sementes de seringueira (Hevea brasiliensis Muell. Arg., tratadas com benlate durante o armazenamento Physiological quality of rubber tree seed treated with benomyl during storage

    Directory of Open Access Journals (Sweden)

    R.D. Vieira

    1995-04-01

    Full Text Available A ocorrência de um período relativamente frio e seco, imediatamente após o período de coleta de sementes de seringueira - Hevea brasiliensis Muell. Arg. (março-abril, levou à realização do presente trabalho com o objetivo de encontrar condições adequadas de preservação da qualidade fisiológica das sementes. Assim, estudou-se a qualidade fisiológica de sementes, tratadas com Benlate (0,1%, e armazenadas em sacos de polietileno transparente, por períodos de 0 a 5 meses, sob condições ambientais de laboratório. Para a avaliação da qualidade fisiológica das sementes, determinou-se a germinação e o vigor através do índice de velocidade de emergência (IVE e do comprimento da parte aérea das plântulas (CPA, em 1987 e 1988. A germinação e o IVE foram obtidos sob duas condições ambientais diferentes, ambiente do laboratório e em germinador a 30°C. O tratamento fungicida utilizado não exerceu efeito significativo na preservação das sementes. A germinação, o IVE e o CPA reduziram-se com o prolongamento do armazenamento. Os valores da germinação foram baixo desde o início dos trabalhos, o que pode ser atribuído ao teor de água inicial, que estava abaixo de 30%. O teste de germinação de sementes dessa espécie pode ser feito utilizando-se areia como substrato, a 30°C e com a contagem final aos 20 dias após a instalação do teste.After rubber tree (Hevea brasiliensis Muell. Arg. seeds reach the physiological maturity, there is a relatively dry and cold period in the State of São Paulo, which led to the present work, in order to evaluate environmental conditions that maintain physiological seed quality. The physiological quality of rubber tree seeds, treated with benomyl (0,1% and stored in plastic bags, was studied up to 5 months, during 87/88. The seed bags were kept under laboratory conditions during the whole storage period. To evaluate physiological seed quality, germination and vigor (germination speed

  7. Identification of a new class of lipid droplet-associated proteins in plants

    Science.gov (United States)

    Lipid droplets in plants (also known as oil bodies, lipid bodies or oleosomes) are well characterized in seeds, and oleosins, the major proteins associated with their surface, were shown to be important for stabilizing lipid droplets during seed desiccation and rehydration. However, lipid droplets ...

  8. Galactinol as marker for seed longevity

    NARCIS (Netherlands)

    Souza Vidigal, De D.; Willems, L.A.J.; Arkel, van J.; Dekkers, S.J.W.; Hilhorst, H.W.M.; Bentsink, L.

    2016-01-01

    Reduced seed longevity or storability is a major problem in seed storage and contributes to increasedcosts in crop production. Here we investigated whether seed galactinol contents could be predictive forseed storability behavior in Arabidopsis, cabbage and tomato. The analyses revealed a positive

  9. Effect of ethanolic flax (Linum usitatissimum L.) extracts on lipid oxidation and changes in nutritive value of frozen-stored meat products.

    Science.gov (United States)

    Waszkowiak, Katarzyna; Szymandera-Buszka, Krystyna; Hęś, Marzanna

    2014-01-01

    Flaxseed (Linum usitatissimum L.) is an important source of phenolic compounds, mainly lignans. Antioxidant capacities of flaxseed extracts that contain the compounds have been reported earlier. However, there is a lack of accessible information about their activity against lipid oxidation in meat products. Therefore, the effect of ethanolic flaxseed extracts (EFEs) on lipid stability and changes in nutritive value of frozen-stored meat products (pork meatballs and burgers) was determined. EFEs from three Polish flax varieties (Szafir, Oliwin, Jantarol) were applied in the study. During 150-day storage of meat products, the lipid oxidation (peroxide and TBARS value) and thiamine retention were periodically monitored, alongside with methionine and lysine availability and protein digestibility. The addition of EFEs significantly limited lipid oxidation in stored meatballs and burgers. EFE from brown seeds of Szafir var. was superior to the others from golden seeds of Jantarol and Oliwin. Moreover, the extracts reduced changes in thiamine and available lysine content, as well as protein digestibility, during storage time. The effect of EFE addition on available methionine retention was limited. The ethanolic flaxseed extracts exhibit antioxidant activity during frozen storage of meat products. They can be utilized to prolong shelf-life of the products by protecting them against lipid oxidation and deterioration of their nutritional quality. However, antioxidant efficiency of the extracts seems to depend on chemical composition of raw material (flax variety). Further investigations should be carried on to explain the issue.

  10. Influence of chain length of pyrene fatty acids on their uptake and metabolism by Epstein-Barr-virus-transformed lymphoid cell lines from a patient with multisystemic lipid storage myopathy and from control subjects.

    Science.gov (United States)

    Radom, J; Salvayr