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Sample records for schistosomula freshly isolated

  1. Cultivation, isolation and characterization of bacteriocin from fresh ...

    African Journals Online (AJOL)

    This study focus on cultivation, isolation and characterization of Bacteriocin from fresh cow milk (FCM) and fresh cow meat (FMS) samples obtained from Lapai Market in Niger State, Nigeria. Potential bacteriocinogenic bacteria were screened with agar diffusion method on culture plates seeded with Staphylococcus and ...

  2. Antibiotic resistance of Staphylococcus aureus isolated from fresh ...

    African Journals Online (AJOL)

    Antibiotic resistance of Staphylococcus aureus isolated from fresh cow milk in settled ... produced alpha haemolysin, 45.5% (n=25) produced beta haemolysin and ... resistant strains of S. aureus of animal and human biotypes and can serve as ...

  3. The skin migratory stage of the schistosomulum of Schistosoma mansoni has a surface showing greater permeability and activity in membrane internalisation than other forms of skin or mechanical schistosomula.

    Science.gov (United States)

    DE Jesus Jeremias, Wander; DA Cunha Melo, Jose Renan; Baba, Elio Hideo; Coelho, Paulo Marcos Zech; Kusel, John Robert

    2015-08-01

    Skin schistosomula can be prepared by collecting them after isolated mouse skin have been penetrated by cercariae in vitro. The schistosomula can also migrate out of isolated mouse skin penetrated by cercariae in vitro and from mouse skin penetrated by cercariae in vivo. Schistosomula can also be produced from cercariae applied through a syringe or in a vortex. When certain surface properties of the different forms of schistosomula were compared, those migrating from mouse skin penetrated by cercariae in vivo or in vitro had greatly increased permeability to membrane impermeant molecules such as Lucifer yellow and high molecular weight dextrans. These migrating forms also possessed surfaces which showed greatly enhanced uptake into internal membrane vesicles of the dye FM 143, a marker for endocytosis. This greatly enhanced activity and permeability of the surfaces of tissue migrating schistosomula is likely to be of great importance in the adaptation to the new host.

  4. Bronchodilatory effect of deep inspiration in freshly isolated sheep lungs.

    Science.gov (United States)

    Wong, William D; Wang, Lu; Paré, Peter D; Seow, Chun Y

    2017-02-01

    Taking a big breath is known to reverse bronchoconstriction induced by bronchochallenge in healthy subjects; this bronchodilatory effect of deep inspiration (DI) is diminished in asthmatics. The mechanism underlying the DI effect is not clear. Observations from experiments using isolated airway smooth muscle (ASM) preparations and airway segments suggest that straining of ASM due to DI could lead to bronchodilation, possibly due to strain-induced reduction in ASM contractility. However, factors external to the lung cannot be excluded as potential causes for the DI effect. Neural reflex initiated by stretch receptors in the lung are known to inhibit the broncho-motor tone and enhance vasodilatation; the former directly reduces airway resistance, and the latter facilitates removal of contractile agonists through the bronchial circulation. If the DI effect is solely mediated by factors extrinsic to the lung, the DI effect would be absent in isolated, nonperfused lungs. Here we examined the DI effect in freshly isolated, nonperfused sheep lungs. We found that imposition of DI on isolated lungs resulted in significant bronchodilation, that this DI effect was present only after the lungs were challenged with a contractile agonist (acetylcholine or histamine), and that the effect was independent of the difference in lung volume observed pre- and post-DI. We conclude that a significant portion of the bronchodilatory DI effect stems from factors internal to the lung related to the activation of ASM. Copyright © 2017 the American Physiological Society.

  5. The requirement for freshly isolated human colorectal cancer (CRC) cells in isolating CRC stem cells.

    Science.gov (United States)

    Fan, F; Bellister, S; Lu, J; Ye, X; Boulbes, D R; Tozzi, F; Sceusi, E; Kopetz, S; Tian, F; Xia, L; Zhou, Y; Bhattacharya, R; Ellis, L M

    2015-02-03

    Isolation of colorectal cancer (CRC) cell populations enriched for cancer stem cells (CSCs) may facilitate target identification. There is no consensus regarding the best methods for isolating CRC stem cells (CRC-SCs). We determined the suitability of various cellular models and various stem cell markers for the isolation of CRC-SCs. Established human CRC cell lines, established CRC cell lines passaged through mice, patient-derived xenograft (PDX)-derived cells, early passage/newly established cell lines, and cells directly from clinical specimens were studied. Cells were FAC-sorted for the CRC-SC markers CD44, CD133, and aldehyde dehydrogenase (ALDH). Sphere formation and in vivo tumorigenicity studies were used to validate CRC-SC enrichment. None of the markers studied in established cell lines, grown either in vitro or in vivo, consistently enriched for CRC-SCs. In the three other cellular models, CD44 and CD133 did not reliably enrich for stemness. In contrast, freshly isolated PDX-derived cells or early passage/newly established CRC cell lines with high ALDH activity formed spheres in vitro and enhanced tumorigenicity in vivo, whereas cells with low ALDH activity did not. PDX-derived cells, early passages/newly established CRC cell lines and cells from clinical specimen with high ALDH activity can be used to identify CRC-SC-enriched populations. Established CRC cell lines should not be used to isolate CSCs.

  6. CRYOPRESERVATION OF FRESHLY ISOLATED SYNAPTOSOMES PREPARED FROM THE CEREBRAL-CORTEX OF RATS

    NARCIS (Netherlands)

    GLEITZ, J; BEILE, A; WILFFERT, B; TEGTMEIER, F

    In the present study, we established a cryopreservation method for freshly isolated synaptosomes prepared from the cerebral cortex of rats. Freshly prepared synaptosomes were either shock-frozen or frozen under temperature-controlled conditions using a programmable temperature controller. Each group

  7. Congestive heart failure effects on atrial fibroblast phenotype: differences between freshly-isolated and cultured cells.

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    Kristin Dawson

    Full Text Available Fibroblasts are important in the atrial fibrillation (AF substrate resulting from congestive heart failure (CHF. We previously noted changes in in vivo indices of fibroblast function in a CHF dog model, but could not detect changes in isolated cells. This study assessed CHF-induced changes in the phenotype of fibroblasts freshly isolated from control versus CHF dogs, and examined effects of cell culture on these differences.Left-atrial fibroblasts were isolated from control and CHF dogs (ventricular tachypacing 240 bpm × 2 weeks. Freshly-isolated fibroblasts were compared to fibroblasts in primary culture. Extracellular-matrix (ECM gene-expression was assessed by qPCR, protein by Western blot, fibroblast morphology with immunocytochemistry, and K(+-current with patch-clamp. Freshly-isolated CHF fibroblasts had increased expression-levels of collagen-1 (10-fold, collagen-3 (5-fold, and fibronectin-1 (3-fold vs. control, along with increased cell diameter (13.4 ± 0.4 µm vs control 8.4 ± 0.3 µm and cell spreading (shape factor 0.81 ± 0.02 vs. control 0.87 ± 0.02, consistent with an activated phenotype. Freshly-isolated control fibroblasts displayed robust tetraethylammonium (TEA-sensitive K(+-currents that were strongly downregulated in CHF. The TEA-sensitive K(+-current differences between control and CHF fibroblasts were attenuated after 2-day culture and eliminated after 7 days. Similarly, cell-culture eliminated the ECM protein-expression and shape differences between control and CHF fibroblasts.Freshly-isolated CHF and control atrial fibroblasts display distinct ECM-gene and morphological differences consistent with in vivo pathology. Culture for as little as 48 hours activates fibroblasts and obscures the effects of CHF. These results demonstrate potentially-important atrial-fibroblast phenotype changes in CHF and emphasize the need for caution in relating properties of cultured fibroblasts to in vivo systems.

  8. Fresh osteochondral allograft transplantation for isolated patellar cartilage injury.

    Science.gov (United States)

    Gracitelli, Guilherme C; Meric, Gokhan; Pulido, Pamela A; Görtz, Simon; De Young, Allison J; Bugbee, William D

    2015-04-01

    The treatment of patellofemoral cartilage injuries can be challenging. Osteochondral allograft (OCA) transplantation has been used as a treatment option for a range of cartilage disorders. To evaluate functional outcomes and survivorship of the grafts among patients who underwent OCA for patellar cartilage injuries. Case series; Level of evidence, 4. An institutional review board-approved OCA database was used to identify 27 patients (28 knees) who underwent isolated OCA transplantation of the patella between 1983 and 2010. All patients had a minimum 2-year follow-up. The mean age of the patients was 33.7 years (range, 14-64 years); 54% were female. Twenty-six (92.9%) knees had previous surgery (mean, 3.2 procedures; range, 1-10 procedures). The mean allograft area was 10.1 cm(2) (range, 4.0-18.0 cm(2)). Patients returned for clinical evaluation or were contacted via telephone for follow-up. The number and type of reoperations were assessed. Any reoperation resulting in removal of the allograft was considered a failure of the OCA transplantation. Patients were evaluated pre- and postoperatively using the modified Merle d'Aubigné-Postel (18-point) scale, the International Knee Documentation Committee (IKDC) pain, function, and total scores, and the Knee Society function (KS-F) score. Patient satisfaction was assessed at latest follow-up. Seventeen of the 28 knees (60.7%) had further surgery after the OCA transplantation; 8 of the 28 knees (28.6%) were considered OCA failures (4 conversions to total knee arthroplasty, 2 conversions to patellofemoral knee arthroplasty, 1 revision OCA, 1 patellectomy). Patellar allografting survivorship was 78.1% at 5 and 10 years and 55.8% at 15 years. Among the 20 knees (71.4%) with grafts in situ, the mean follow-up duration was 9.7 years (range, 1.8-30.1 years). Pain and function improved from the preoperative visit to latest follow-up, and 89% of patients were extremely satisfied or satisfied with the results of the OCA

  9. Hemolytic and urease activities in vibrios isolated from fresh and frozen oysters

    Directory of Open Access Journals (Sweden)

    Renata Albuquerque Costa

    2013-01-01

    Full Text Available INTRODUCTION: The present study aimed to survey the Vibrio microbiota of oysters (Crassostrea rhizophorae obtained from restaurants in Fortaleza, State of Ceará, Brazil, and to identify virulence factors. METHODS: The isolated vibrios were submitted to biochemical identification and were tested for hemolytic and urease activities. RESULTS: The isolated strains belonged to 13 species, with predominance of Vibrio mimicus. Of the strain isolates only from fresh samples, 20.5% and 2.8% showed hemolytic and urease activities, respectively. CONCLUSIONS: The findings support the little-publicized claim that Vibrio species other than V. parahaemolyticus and V. vulnificus can represent a health risk to public health.

  10. Influence of Schistosoma japonicum programmed cell death protein 10 on the growth and development of schistosomula.

    Science.gov (United States)

    Gao, Yan Ru; Huang, Wen Ling; Tang, Chun Lian; Liu, Rong; Zhao, Qin Ping; Ming, Zhen Ping; Dong, Hui Fen

    2018-01-18

    Schistosomiasis caused by Schistosoma japonicum is among the most serious endemic zoonoses in China. To study interactions between schistosomula, the pre-adult juvenile stage, and hosts, it is important to study the functions of key genes involved in schistosomula growth and development. Programmed cell death protein 10 (pcdp10) is an important apoptosis-related gene with various biological functions. This study described the molecular characterization of S. japonicum PCDP10 (SjPCDP10) and evaluated its functions in schistosomula. Real-time quantitative polymerase chain reaction (qPCR) and western blot were used to detect Sjpcdp10 mRNA and protein levels, respectively, at different developmental stages. Immunolocalization was performed to determine SjPCDP10 expression in the parasite. RNA interference (RNAi) experiments were used to assess gene functions associated with SjPCDP10 in schistosomula growth and development. Real-time qPCR revealed that Sjpcdp10 was expressed during all investigated developmental stages and upregulated during schistosomula growth and development. Histochemical localization showed that SjPCDP10 was mainly distributed in the teguments of schistosomula in all investigated stages and part of the parenchymal area of 14-, 18-, and 21-day-old schistosomula. Following Sjpcdp10 knockdown by RNAi, the lengths, widths, areas, and volumes of schistosomula were significantly lower than those in the control group. Scanning electron microscopy showed that the body surfaces of schistosomula subjected to RNAi were seriously damaged, with few tegumental spines and sensory papillae. Transmission electron microscopy indicated that the teguments of Sjpcdp10-knockdown schistosomula were incomplete, the number of layers was reduced, and the thickness decreased significantly as compared with those in the control group. Furthermore, terminal deoxynucleotidyl transferase dUTP nick-end labelling results showed that the rate of apoptosis in Sjpcdp10-knockdown

  11. Mechanisms of evasion of Schistosoma mansoni schistosomula to the lethal activity of complement

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    F. Juarez Ramalho-Pinto

    1992-01-01

    Full Text Available Schistosomula of Schistosoma mansoni became resistant to antibody-dependent complement damage in vitro after pre-incubation with normal human erythrocytes (NHuE whatever the ABO or Rh blood group. Resistant parasites were shown to acquire host decay accelerating factor (DAF , a 70 kDa glycoprotein attached to the membrane of NHue by a GPI anchor. IgG2a mAb anti-human DAF (IA10 immunoprecipitated a 70 kDa molecule from 125I-labeled schistosomula pre-incubated with NHuE and inhibited their resistance to complement-dependent killing in vtro. Incubationof schistosomula with erytrocytes from patients with paroxsimal nocturnal hemoglobinuria (PNHE or SRBC, wich are DAF-deficient, did not protect the parasites from complement lesion. Supernatant of 100,000 x g collected from NHuE incubated for 24 h in defined medium was shown to contain a soluble form of DAF and to protect schistosomula from complement killing. Schistosomula treated with trypsin before incubation with NHuE ghosts did not become resistant to complement damage. On the other hand, pre-treatment with chymotrypsin did not interfere with the acquisition of resistance by the schistosomula. These results indicate that, in vitro, NHuE DAF can be transferred to schistosomula in a soluble form and that the binding of this molecule to the parasite surface is dependent upon trypsin-sensitive chymotrypsin-insensitive polipeptide(s present on the surface of the worm.

  12. Isolation, molecular identification and quinolone-susceptibility testing of Arcobacter spp. isolated from fresh vegetables in Spain.

    Science.gov (United States)

    González, Ana; Bayas Morejón, Isidro Favián; Ferrús, María Antonia

    2017-08-01

    Some species of the Arcobacter genus are considered emerging foodborne and waterborne enteropathogens. However, the presence of Arcobacter spp. in vegetables very little is known, because most studies have focused on foods of animal origin. On the other hand, quinolones are considered as first-line drugs for the treatment of infection by campylobacteria in human patients, but few data are currently available about the resistance levels to these antibiotics among Arcobacter species. Therefore, the aim of this study was to investigate the presence and diversity of arcobacters isolated from fresh vegetables such as lettuces, spinaches, chards and cabbages. Resistance to quinolones of the isolates was also investigated. One hundred fresh vegetables samples purchased from seven local retail markets in Valencia (Spain) during eight months were analysed. The study included 41 lettuces, 21 spinaches, 34 chards and 4 cabbages. Samples were analysed by culture and by molecular methods before and after enrichment. By culture, 17 out of 100 analysed samples were Arcobacter positive and twenty-five isolates were obtained from them. Direct detection by PCR was low, with only 4% Arcobacter spp. positive samples. This percentage increased considerably, up 20%, after 48 h enrichment. By polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), 17 out of the 25 isolates were identified as A. butzleri and 8 as A. cryaerophilus. Only two A. butzleri isolates showed resistance to levofloxacin and ciprofloxacin. The sequencing of a fragment of the QRDR region of the gyrA gene from the quinolones-resistant isolates revealed the presence of a mutation in position 254 of this gene (C-T transition). This study is the first report about the presence of pathogenic species of Arcobacter spp. in chards and cabbages and confirms that fresh vegetables can act as transmission vehicle to humans. Moreover, the presence of A. butzleri quinolone resistant in vegetables could

  13. Isolation and determination of cultural characteristics of a new highly CO2 tolerant fresh water microalgae

    International Nuclear Information System (INIS)

    Yue Lihong; Chen Weigong

    2005-01-01

    Fresh water microalgae, which has high CO 2 tolerance, were isolated and its cultural characteristics were investigated. The ZY-1 strain was identified as genus Chlorella. It showed maximum growth at 10% (v/v) CO 2 enriched air flowing condition, and a good growth rate in a broad range of physically controllable conditions, including CO 2 concentration up to 70% (v/v), CO 2 enriched air flow rate, temperature and pH value. The results indicated the feasibility of the ZY-1 strain for fixing CO 2 from stack gases

  14. The inhibitory activity of Lactic acid bacteria isolated from fresh cow cheese

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    Nevijo Zdolec

    2007-04-01

    Full Text Available Lactic acid bacteria are the constituent part of milk microbial flora that could influence the safety of dairy products due production of organic acids, hydrogen peroxide, carbon dioxide and bacteriocins. Taking this in consideration, the objective of this study was to investigate the composition of lactic acid bacteria population in fresh cow cheeses taken from local markets, as well as their antimicrobial capacity. Lactic acid bacteria counts were determined according to ISO 1524:1998 method, biochemical determination using API 50 CHL system, and inhibitory activity against L. monocytogenes NCTC 10527 by agar well diffusion assay. Lactic acid bacteria count in fresh cow cheeses (n=10 ranged from 5.87 to 8.38 log10 CFU g-1. Among 52 MRS isolates collected, 61.54 % were assigned to the Lactococcus lactis subsp. Lactis species, 23.07 % Lactobacillus helveticus, 11.54 % Leuconostoc mesenteroides subsp. cremoris and 3.85 % Leuconostoc mesenteroides subsp. mesenteroides. Antilisterial activity was found in 18 isolates.

  15. Mycoflora and aflatoxin/fumonisin production by fungal isolates from freshly harvested corn hybrids

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    Almeida Adriana P.

    2000-01-01

    Full Text Available The mycoflora of 3 hybrids of freshly harvested corn grains collected from three regions of the state of São Paulo, Brazil (Assis, Capão Bonito and Ribeirão Preto was investigated. A total of 66 samples were analyzed focusing on the influence of abiotic factors (moisture content, water activity, temperature and rainfall on both the prevalence of Aspergillus flavus and Fusarium moniliforme, and the ability of these genera isolates to produce aflatoxins and fumonisins, respectively. In the three surveyed regions, the fungal population comprised mainly Fusarium spp., Penicillium spp., Aspergillus spp. and 2 others filamentous fungal genera, which were isolated from corn kernels showing water activity of 0.30 to 0.99 and moisture content of 5.0% to 20.2%. Among the genera Fusarium and Aspergillus, the most frequent species were F. moniliforme and A. flavus, respectively. Concerning the toxigenic potential of F. moniliforme, all isolated strains (40 produced fumonisins at 20 mug/g to 2168 mug/g (FB1 and/or 10 mug/g to 380 mug/g (FB2. From the 10 A. flavus isolates, 6 strains (60.0% produced aflatoxins at 615 mug/kg to 30.750 mug/kg (AFB1 and/or 11 mug/kg to 22 mug/kg (AFB2.

  16. Efficacy of fresh leaf extracts of Spondias mombin against some clinical bacterial isolates from typhoid patients

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    Olukemi Aromolaran

    2014-12-01

    Full Text Available Objective: To evaluate the phytochemical properties and antibacterial activity of methanol, acetone, ethanol and aqueous extracts of fresh leaves of Spondias mombin (S. mombin on some clinical bacterial isolates. Methods: Clean and fresh leaves of S. mombin were collected in Ondo, Southwestern Nigeria. The leaves were blended, extracted with methanol, acetone, ethanol and water. The extracts were evaporated to dryness using rotary evaporator and tested for the presence of saponins, tannins, cardiac glycoside, terpenoids, flavonoids, reducing sugars, volatile oils, alkaloids and glycoside. The extract were tested against Gram-negative bacteria Klebsiella pneumonia, Serratia marcescens, Salmonella typhi and Enterobacter aerogens; Gram-positive bacteria Staphylococcus aureus by observing the zones of inhibition using agar well diffusion assay. Results: The study showed that the leaves contained saponins, tannins, flavonoids, alkaloids and glycoside. All the solvent extracts showed activity against all the test bacteria. The methanol extract also showed the highest activity against Enterobacter aerogens, zone of diameter (15.00 依1.89 mm, while the ethanol extract showed the highest activity against Staphylococcus aureus with zone of diameter (12.50依1.50 mm. The acetone extract showed the highest activity against Salmonella typhi, zone of diameter (17.50依0.29 mm followed by methanol extract showing zone of diameter (15.67依1.01 mm. The acetone extract showed the highest activity against Klebsiella pneumonia (15.17依0.67 mm, while the aqueous extract shows the highest activity against Serratia marcescens (14.67依2.68 mm. The minimum inhibitory concentration of the leaf extracts ranged between 10-90 mg/mL. Conclusions: This study showed that the aqueous and organic solvents extract of fresh leaves of S. mombin has anti-microbial activity against all the tested organisms.

  17. Development of a potential probiotic fresh cheese using two Lactobacillus salivarius strains isolated from human milk.

    Science.gov (United States)

    Cárdenas, Nivia; Calzada, Javier; Peirotén, Angela; Jiménez, Esther; Escudero, Rosa; Rodríguez, Juan M; Medina, Margarita; Fernández, Leónides

    2014-01-01

    Cheeses have been proposed as a good alternative to other fermented milk products for the delivery of probiotic bacteria to the consumer. The objective of this study was to assess the survival of two Lactobacillus salivarius strains (CECT5713 and PS2) isolated from human milk during production and storage of fresh cheese for 28 days at 4°C. The effect of such strains on the volatile compounds profile, texture, and other sensorial properties, including an overall consumer acceptance, was also investigated. Both L. salivarius strains remained viable in the cheeses throughout the storage period and a significant reduction in their viable counts was only observed after 21 days. Globally, the addition of the L. salivarius strains did not change significantly neither the chemical composition of the cheese nor texture parameters after the storage period, although cheeses manufactured with L. salivarius CECT5713 presented significantly higher values of hardness. A total of 59 volatile compounds were identified in the headspace of experimental cheeses, and some L. salivarius-associated differences could be identified. All cheeses presented good results of acceptance after the sensory evaluation. Consequently, our results indicated that fresh cheese can be a good vehicle for the two L. salivarius strains analyzed in this study.

  18. Development of a Potential Probiotic Fresh Cheese Using Two Lactobacillus salivarius Strains Isolated from Human Milk

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    Nivia Cárdenas

    2014-01-01

    Full Text Available Cheeses have been proposed as a good alternative to other fermented milk products for the delivery of probiotic bacteria to the consumer. The objective of this study was to assess the survival of two Lactobacillus salivarius strains (CECT5713 and PS2 isolated from human milk during production and storage of fresh cheese for 28 days at 4°C. The effect of such strains on the volatile compounds profile, texture, and other sensorial properties, including an overall consumer acceptance, was also investigated. Both L. salivarius strains remained viable in the cheeses throughout the storage period and a significant reduction in their viable counts was only observed after 21 days. Globally, the addition of the L. salivarius strains did not change significantly neither the chemical composition of the cheese nor texture parameters after the storage period, although cheeses manufactured with L. salivarius CECT5713 presented significantly higher values of hardness. A total of 59 volatile compounds were identified in the headspace of experimental cheeses, and some L. salivarius-associated differences could be identified. All cheeses presented good results of acceptance after the sensory evaluation. Consequently, our results indicated that fresh cheese can be a good vehicle for the two L. salivarius strains analyzed in this study.

  19. Immunization of mice with gamma-irradiated intramuscularly injected schistosomula of Schistosoma mansoni

    International Nuclear Information System (INIS)

    Bickle, Q.D.; Taylor, M.G.; Doenhoff, M.J.; Nelson, G.S.

    1979-01-01

    The parameters involved in the induction of resistance against Schistosoma mansoni by injection of irradiated, artificially transformed schistosomula were studied in mice. Single intramuscular injections of 500 schistosomula exposed to radiation doses in the range 2.3 to 160 krad. resulted in significant protection ( in the range 20 to 50% as assessed by reduced worm burdens) against a challenge infection administered at intervals from 3 to 24 weeks post-vaccination. However, schistosomular irradiated with 20 krad. consistently resulted in better protection than those exposed to either higher or lower radiation doses despite the persistence of stunted adults from the infections irradiated with 2.3 krad. Vaccination with 40 krad. schistosomula resulted in significant protection in terms of reduced worm and tissue egg burdens and increased survival following lethal challenge. Varying the number of irradiated schistosomula, the frequency and route of their administration, the site of challenge and the strain of host all failed to enhance the level of resistance. However, percutaneously applied, irradiated cercariae were found to be more effective in stimulating resistance (60%) than intramuscularly injected, irradiated schistosomula (40%). (author)

  20. Antimicrobial resistance in Escherichia coli and Salmonella spp. isolates from fresh produce and the impact to food safety.

    Science.gov (United States)

    Vital, Pierangeli G; Caballes, Marie Bernadine D; Rivera, Windell L

    2017-09-02

    Foodborne diseases associated with fresh produce consumption have escalated worldwide, causing microbial safety of produce of critical importance. Bacteria that have increasingly been detected in fresh produce are Escherichia coli and Salmonella spp., both of which have been shown to progressively display antimicrobial resistance. The study focused on the assessment of antimicrobial resistance of these enteric bacteria from different kinds of fresh produce from various open air markets and supermarkets in the Philippines. Using the disk diffusion assay on a total of 50 bacterial isolates obtained from 410 fresh produce surveyed, monoresistance to tetracycline was observed to be the most prevalent (38%), followed by multidrug resistance to tetracycline, chloramphenicol, ciprofloxacin, and nalidixic acid (4%), and lastly by dual resistance to tetracycline and chloramphenicol (2%). Using multiplex and simplex polymerase chain reaction (PCR) assays, tetA (75%) and tetB (9%) were found in tetracycline resistant isolates, whereas catI (67%) and catIII (33%) were detected in chloramphenicol resistant isolates. Sequence analysis of gyr and par genes from the ciprofloxacin and nalidixic acid resistant isolates revealed different mutations. Based on the results, fresh produce act as a reservoir of these antibiotic resistant bacteria which may pose health threat to consumers.

  1. Development of Schistosoma incognitum in mice upon intraperitoneal inoculation with irradiated schistosomula

    International Nuclear Information System (INIS)

    Bhilegaonkar, N.G.; Sahasrabudhe, V.K.

    1987-01-01

    As a prelude to the study of the immunizing potential of gamma-irradiated Schistosoma incognitum schistosomula, experiments were conducted to study the effect of different doses of gamma irradiation (1,3,5 and 10 kr) on the development and survival of S. incognitum in mice, and its attendant pathology. The present experiments suggested that 3 and 5 kr irradiation doses can be safely used for irradiating schistosomula for immunization experiments in mice as the worms will not mature and therefore no harm will be caused which is mainly due to the eggs. (author). 7 refs

  2. Identification and measurement of rat eosinophil phospholipase D. Its activity on schistosomula phospholipids

    International Nuclear Information System (INIS)

    Lempereur, C.; Capron, M.; Capron, A.

    1980-01-01

    A sensitive assay, using [ 14 C]lecithin as a substrate, has been developed for the measurement of phospholipase activity in rat peritoneal polymorphonuclear leukocytes. Cell extracts were found to contain a phospholipase D activity and indirect evidence suggested that eosinophils are responsible for the cleavage of lecithin. Intact peritoneal cells were also able to hydrolyze exogenous [ 14 C]lecithin in vitro. When [ 3 H]choline-labeled schistosomula were used as targets in antibody-dependent cytotoxicity experiments, the radioactivity of lecithin decreased more rapidly in a complete cytotoxicity system than in controls, suggesting that hydrolysis of schistosomula phospholipids occurred during the killing process. (Auth.)

  3. A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels.

    Science.gov (United States)

    Brzica, Hrvoje; Abdullahi, Wazir; Reilly, Bianca G; Ronaldson, Patrick T

    2018-05-07

    The blood-brain barrier (BBB) is a dynamic barrier tissue that responds to various pathophysiological and pharmacological stimuli. Such changes resulting from these stimuli can greatly modulate drug delivery to the brain and, by extension, cause considerable challenges in the treatment of central nervous system (CNS) diseases. Many BBB changes that affect pharmacotherapy, involve proteins that are localized and expressed at the level of endothelial cells. Indeed, such knowledge on BBB physiology in health and disease has sparked considerable interest in the study of these membrane proteins. From a basic science research standpoint, this implies a requirement for a simple but robust and reproducible method for isolation of microvessels from brain tissue harvested from experimental animals. In order to prepare membrane samples from freshly isolated microvessels, it is essential that sample preparations be enriched in endothelial cells but limited in the presence of other cell types of the neurovascular unit (i.e., astrocytes, microglia, neurons, pericytes). An added benefit is the ability to prepare samples from individual animals in order to capture the true variability of protein expression in an experimental population. In this manuscript, details regarding a method that is utilized for isolation of rat brain microvessels and preparation of membrane samples are provided. Microvessel enrichment, from samples derived, is achieved by using four centrifugation steps where dextran is included in the sample buffer. This protocol can easily be adapted by other laboratories for their own specific applications. Samples generated from this protocol have been shown to yield robust experimental data from protein analysis experiments that can greatly aid the understanding of BBB responses to physiological, pathophysiological, and pharmacological stimuli.

  4. Enterobacter and Klebsiella species isolated from fresh vegetables marketed in Valencia (Spain) and their clinically relevant resistances to chemotherapeutic agents.

    Science.gov (United States)

    Falomir, María Pilar; Rico, Hortensia; Gozalbo, Daniel

    2013-12-01

    Occurrence of antibiotic-resistant pathogenic or commensal enterobacteria in marketed agricultural foodstuffs may contribute to their incorporation into the food chain and constitutes an additional food safety concern. In this work, we have determined the clinically relevant resistances to 11 common chemotherapeutic agents in Enterobacter and Klebsiella isolates from fresh vegetables from various sources (supermarkets and greengrocers' shops in Valencia, Spain). A total of 96 isolates were obtained from 160 vegetables analyzed (50% positive samples): 68 Enterobacter isolates (59 E. cloacae, two E. aerogenes, two E. cancerogenus, one E. gergoviae, and four E. sakazakii, currently Cronobacter spp.), and 28 Klebsiella isolates (19 K. oxytoca and 9 K. pneumoniae). Only seven isolates were susceptible to all agents tested, and no resistances to ceftazidime, ciprofloxacin, gentamicin, and chloramphenicol were detected. Most isolates were resistant to amoxicillin/clavulanic acid (74 [58 Enterobacter and 16 Klebsiella]) or to ampicillin (80 [55/25]). Other resistances were less frequent: nitrofurantoin (13 isolates [12/1]), tetracycline (6 [5/1]), co-trimoxazole (3 [3/0]), cefotaxime (1 [1/0]), and streptomycin (2 [1/1]). Multiresistant isolates to two (56 [41/15]), three (10 E. cloacae isolates), four (one E. cloacae and one K. pneumoniae isolate), and five (two E. cloacae isolates) chemotherapeutic agents were also detected. The presence of potential pathogens points to marketed fresh produce, which often is eaten raw, as a risk factor for consumer health. In addition, these results support the usefulness of these bacterial species as indicators of the spreading of antibiotic resistances into the environment, particularly in the food chain, and suggest their role as carriers of resistance determinants from farms to consumers, which may constitute an additional "silent" food safety concern. Therefore, there is a need to improve the hygienic quality of marketed fresh

  5. siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts.

    Science.gov (United States)

    Lokossou, Adjimon Gatien; Toufaily, Chirine; Vargas, Amandine; Barbeau, Benoit

    2016-09-20

    Human primary villous cytotrophoblasts are a very useful source of primary cells to study placental functions and regulatory mechanisms, and to comprehend diseases related to pregnancy. In this protocol, human primary villous cytotrophoblasts freshly isolated from placentas through a standard DNase/trypsin protocol are microporated with small interfering RNA (siRNA). This approach provided greater efficiency for siRNA transfection when compared to a lipofection-based method. Transfected cells can subsequently be analyzed by standard Western blot within a time frame of 3-4 days post-transfection. In addition, using cultured primary villous cytotrophoblasts, Electrophoretic Mobility Shift Assay (EMSA) analysis was optimized and performed on extracts from days 1 to 4. The use of these cultured primary cells and the protocol described allow for an evaluation of the implication of specific genes and transcription factors in the process of villous cytotrophoblast differentiation into a syncytiotrophoblast-like cell layer. However, the limited time span allowable in culture precludes the use of methods requiring more time, such as generation of a stable cell population. Therefore testing of this cell population requires highly optimized gene transfer protocols.

  6. Two-photon excited autofluorescence imaging of freshly isolated frog retinas.

    Science.gov (United States)

    Lu, Rong-Wen; Li, Yi-Chao; Ye, Tong; Strang, Christianne; Keyser, Kent; Curcio, Christine A; Yao, Xin-Cheng

    2011-06-01

    The purpose of this study was to investigate cellular sources of autofluorescence signals in freshly isolated frog (Rana pipiens) retinas. Equipped with an ultrafast laser, a laser scanning two-photon excitation fluorescence microscope was employed for sub-cellular resolution examination of both sliced and flat-mounted retinas. Two-photon imaging of retinal slices revealed autofluorescence signals over multiple functional layers, including the photoreceptor layer (PRL), outer nuclear layer (ONL), outer plexiform layer (OPL), inner nuclear layer (INL), inner plexiform layer (IPL), and ganglion cell layer (GCL). Using flat-mounted retinas, depth-resolved imaging of individual retinal layers further confirmed multiple sources of autofluorescence signals. Cellular structures were clearly observed at the PRL, ONL, INL, and GCL. At the PRL, the autofluorescence was dominantly recorded from the intracellular compartment of the photoreceptors; while mixed intracellular and extracellular autofluorescence signals were observed at the ONL, INL, and GCL. High resolution autofluorescence imaging clearly revealed mosaic organization of rod and cone photoreceptors; and sub-cellular bright autofluorescence spots, which might relate to connecting cilium, was observed in the cone photoreceptors only. Moreover, single-cone and double-cone outer segments could be directly differentiated.

  7. Schistosoma mansoni: migration and attrition of irradiated and challenge schistosomula in the mouse

    Energy Technology Data Exchange (ETDEWEB)

    Mastin, A.J.; Wilson, R.A. (York Univ. (UK)); Bickle, Q.D. (Winches Farm Field Station, St. Albans (UK))

    1983-08-01

    The fate of irradiated, immunizing cercariae and challenge schistosomula was investigated in mice using a quantitative, histological technique which would appear to be more efficient in estimating parasite numbers in skin and lungs than does the tissue mincing and incubation recovery technique used previously by other workers. There was evidence for slight retardation of irradiated parasite migration in skin, but death of schistosomula in the skin appeared negligible. The majority of irradiated parasites remained in the lungs until at least day 21 after infection, and that most schistosomula observed at this time were dead. In mice immunized with irradiated cercariae there was no evidence of attrition of the challenge infection in the skin. The migration profile of challenge parasites in the lungs of immunized animals was not significantly different from that of normal parasites in the lungs of naive animals. Dead challenge parasites were observed in the lungs but their numbers were not considered great enough to account for the final attrition measured by perfusion. It is suggested that the remainder of the attrition of challenge schistosomula may occur in the systemic and/or hepatic portal circulation.

  8. Schistosoma mansoni: migration and attrition of irradiated and challenge schistosomula in the mouse

    International Nuclear Information System (INIS)

    Mastin, A.J.; Wilson, R.A.; Bickle, Q.D.

    1983-01-01

    The fate of irradiated, immunizing cercariae and challenge schistosomula was investigated in mice using a quantitative, histological technique which would appear to be more efficient in estimating parasite numbers in skin and lungs than does the tissue mincing and incubation recovery technique used previously by other workers. There was evidence for slight retardation of irradiated parasite migration in skin, but death of schistosomula in the skin appeared negligible. The majority of irradiated parasites remained in the lungs until at least day 21 after infection, and that most schistosomula observed at this time were dead. In mice immunized with irradiated cercariae there was no evidence of attrition of the challenge infection in the skin. The migration profile of challenge parasites in the lungs of immunized animals was not significantly different from that of normal parasites in the lungs of naive animals. Dead challenge parasites were observed in the lungs but their numbers were not considered great enough to account for the final attrition measured by perfusion. It is suggested that the remainder of the attrition of challenge schistosomula may occur in the systemic and/or hepatic portal circulation. (author)

  9. Pathology associated with vaccination against Schistosoma mansoni in mice using cryopreserved radiation attenuated schistosomula

    International Nuclear Information System (INIS)

    James, E.R.; Dobinson, A.R.

    1985-01-01

    Twenty-one mice were injected intramuscularly with 2000 Schistosoma mansoni schistosomula irradiated at 20 krad and cryopreserved; three mice were killed on each of day 0, 2, 5, 9, 19, 28 and 44 days after infection and muscle from the site of injection in the left hind leg, the lungs and livers removed for histological examination. Schistosomula were seen in sections from the leg muscle from days 0 to 19 inclusive, in the lungs from day 2 to day 28 inclusive and in the livers from days 9 to 28 inclusive. Most schistosomula were seen in sections of the leg muscle with considerably fewer parasites occurring in the lungs and especially the livers. Granulomatous reactions comprising eosinophils, polymorphs, plasma cells and macrophages were first seen in the leg muscle on day 2, in the lungs on day 5 and in the liver on day 19. The peak inflammatory reactions appeared to occur between days 5 and 9, 9 and 19 and 28 and 44 respectively in the three tissues. The pathology is discussed in relation to the dose of irradiation required to attenuate the schistosomula for optimal immunogenicity. (author)

  10. Extracellular acidosis activates ASIC-like channels in freshly isolated cerebral artery smooth muscle cells.

    Science.gov (United States)

    Chung, Wen-Shuo; Farley, Jerry M; Swenson, Alyssa; Barnard, John M; Hamilton, Gina; Chiposi, Rumbidzayi; Drummond, Heather A

    2010-05-01

    Recent studies suggest that certain acid-sensing ion channels (ASIC) are expressed in vascular smooth muscle cells (VSMCs) and are required for VSMC functions. However, electrophysiological evidence of ASIC channels in VSMCs is lacking. The purpose of this study was to test the hypothesis that isolated cerebral artery VSMCs express ASIC-like channels. To address this hypothesis, we used RT-PCR, Western blotting, immunolabeling, and conventional whole cell patch-clamp technique. We found extracellular H(+)-induced inward currents in 46% of cells tested (n = 58 of 126 VSMCs, pH 6.5-5.0). The percentage of responsive cells and the current amplitude increased as the external H(+) concentration increased (pH(6.0), n = 28/65 VSMCs responsive, mean current density = 8.1 +/- 1.2 pA/pF). Extracellular acidosis (pH(6.0)) shifted the whole cell reversal potential toward the Nernst potential of Na(+) (n = 6) and substitution of extracellular Na(+) by N-methyl-d-glucamine abolished the inward current (n = 6), indicating that Na(+) is a major charge carrier. The broad-spectrum ASIC blocker amiloride (20 microM) inhibited proton-induced currents to 16.5 +/- 8.7% of control (n = 6, pH(6.0)). Psalmotoxin 1 (PcTx1), an ASIC1a inhibitor and ASIC1b activator, had mixed effects: PcTx1 either 1) abolished H(+)-induced currents (11% of VSMCs, 5/45), 2) enhanced or promoted activation of H(+)-induced currents (76%, 34/45), or 3) failed to promote H(+) activation in nonresponsive VSMCs (13%, 6/45). These findings suggest that freshly dissociated cerebral artery VSMCs express ASIC-like channels, which are predominantly formed by ASIC1b.

  11. Gender differences in methionine accumulation and metabolism in freshly isolated mouse hepatocytes: Potential roles in toxicity

    International Nuclear Information System (INIS)

    Dever, Joseph T.; Elfarra, Adnan A.

    2009-01-01

    L-Methionine (Met) is hepatotoxic at high concentrations. Because Met toxicity in freshly isolated mouse hepatocytes is gender-dependent, the goal of this study was to assess the roles of Met accumulation and metabolism in the increased sensitivity of male hepatocytes to Met toxicity compared with female hepatocytes. Male hepatocytes incubated with Met (30 mM) at 37 o C exhibited higher levels of intracellular Met at 0.5, 1.0, and 1.5 h, respectively, compared to female hepatocytes. Conversely, female hepatocytes had higher levels of S-adenosyl-L-methionine compared to male hepatocytes. Female hepatocytes also exhibited higher L-methionine-L-sulfoxide levels relative to control hepatocytes, whereas the increases in L-methionine-D-sulfoxide (Met-D-O) levels were similar in hepatocytes of both genders. Addition of aminooxyacetic acid (AOAA), an inhibitor of Met transamination, significantly increased Met levels at 1.5 h and increased Met-D-O levels at 1.0 and 1.5 h only in Met-exposed male hepatocytes. No gender differences in cytosolic Met transamination activity by glutamine transaminase K were detected. However, female mouse liver cytosol exhibited higher methionine-DL-sulfoxide (MetO) reductase activity than male mouse liver cytosol at low (0.25 and 0.5 mM) MetO concentrations. Collectively, these results suggest that increased cellular Met accumulation, decreased Met transmethylation, and increased Met and MetO transamination in male mouse hepatocytes may be contributing to the higher sensitivity of the male mouse hepatocytes to Met toxicity in comparison with female mouse hepatocytes.

  12. N2-fixation by freshly isolated Nostoc from coralloid roots of the cycad Macrozamia riedlei (Fisch. ex Gaud.) Gardn

    International Nuclear Information System (INIS)

    Lindblad, P.; Atkins, C.A.; Pate, J.S.

    1991-01-01

    Nitrogenase (EC 1.7.99.2) activity (acetylene reduction) and nitrogen fixation ( 15 N 2 fixation) were measured in cyanobacteria freshly isolated from the coralloid roots of Macrozamia riedlei (Fisch. ex Gaud.) Gardn. The data indicate that cyanobacteria within cycad coralloid roots are differentiated specifically for symbiotic functioning in a microaerobic environment. Specializations include a high heterocyst frequency, enhanced permeability to O 2 , and a direct dependence on the cycad for substrates to support nitrogenase activity

  13. Adrenaline and reactive oxygen species elicit proteome and energetic metabolism modifications in freshly isolated rat cardiomyocytes

    International Nuclear Information System (INIS)

    Costa, Vera Marisa; Silva, Renata; Tavares, Ludgero Canario; Vitorino, Rui; Amado, Francisco; Carvalho, Felix; Bastos, Maria de Lourdes; Carvalho, Marcia; Carvalho, Rui Albuquerque; Remiao, Fernando

    2009-01-01

    The sustained elevation of plasma and interstitial catecholamine levels, namely adrenaline (ADR), and the generation of reactive oxygen species (ROS) are well recognized hallmarks of several cardiopathologic conditions, like cardiac ischemia/reperfusion (I/R) and heart failure (HF). The present work aimed to investigate the proteomics and energetic metabolism of cardiomyocytes incubated with ADR and/or ROS. To mimic pathologic conditions, freshly isolated calcium-tolerant cardiomyocytes from adult rat were incubated with ADR alone or in the presence of a system capable of generating ROS [(xanthine with xanthine oxidase) (XXO)]. Two-dimensional electrophoresis with matrix-assisted laser desorption/ionization and time-of-flight mass spectrometer analysis were used to define protein spot alterations in the cardiomyocytes incubated with ADR and/or ROS. Moreover, the energetic metabolism and the activity of mitochondrial complexes were evaluated by nuclear magnetic resonance and spectrophotometric determinations, respectively. The protein extract was mainly constituted by cardiac mitochondrial proteins and the alterations found were included in five functional classes: (i) structural proteins, notably myosin light chain-2; (ii) redox regulation proteins, in particular superoxide dismutase (SOD); (iii) energetic metabolism proteins, encompassing ATP synthase alpha chain and dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; (iv) stress response proteins, like the heat shock proteins; and (v) regulatory proteins, like cytochrome c and voltage-dependent anion channel 1. The XXO system elicited alterations in cardiac contractile proteins, as they showed high levels of cleavage, and also altered energetic metabolism, through increased lactate and alanine levels. The cardiomyocytes incubation with ADR resulted in an accentuated increase in mitochondrial complexes activity and the decrease in alanine/lactate ratio, thus reflecting a high

  14. Glucose metabolite patterns as markers of functional differentiation in freshly isolated and cultured mouse mammary epithelial cells

    International Nuclear Information System (INIS)

    Emerman, J.T.; Bartley, J.C.; Bissel, M.J.

    1981-01-01

    In the mammary gland of non-ruminant animals, glucose is utilized in a characteristic and unique way during lacation. By measuring the incorporation of glucose carbon from [U- 14 C]glucose into intermediary metabolitees and metabolic products in mammary epithelia cells from virgin, pregnant, and lacating mice, we domonstrate that glucose metabolite patterns can be used to recognize stages of differentiated function. For these cells, the rates of synthesis of glycogen and lactose, the ratio of lactate to alanine, and the ratio of citrate to malate are important parameters in identifying the degree of expression of differentiation. We further show that these patterns can be used as markers to determine the differentiated state of cultured mammary epithelial cells. Cells maintained on plastic substrates lose their distinctive glucose metabolite patterns while those on floating collagen gels do not. Cells isolated from pregnant mice and cultured on collagen gels have a pattern similar to that of their freshly isolated counter-parts. When isolated from lacating mice, the metabolite patterns of cells cultured on collagen gels are different from that of the cells of origin, and resembles that of freshly isolated cells from pregnant mice. Our findings suggest that the floating collagen gels under the culture conditions used in these experiments provide an environment for the functional expression of the pregnant state, while additional factors are needed for the expression of the lactating state

  15. Endocrine disruption screening by protein and gene expression of vitellogenin in freshly isolated and cryopreserved rainbow trout hepatocytes.

    Science.gov (United States)

    Markell, Lauren K; Mingoia, Robert T; Peterson, Heather M; Yao, Jianhong; Waters, Stephanie M; Finn, James P; Nabb, Diane L; Han, Xing

    2014-08-18

    Xenobiotics may activate the estrogen receptor, resulting in alteration of normal endocrine functions in animals and humans. Consequently, this necessitates development of assay end points capable of identifying estrogenic xenobiotics. In the present study, we screened the potential estrogenicity of chemicals via their ability to induce vitellogenin (VTG) expression in cultured primary hepatocytes from male trout. A routine method for VTG detection measures the secretion of the protein by enzyme-linked immunosorbent assay (ELISA) in freshly isolated trout hepatocytes. However, this lengthy (6 days) culturing procedure requires that hepatocyte isolation is performed each time the assay is run. We optimized this methodology by investigating the utility of cryopreserved hepatocytes, shortening the incubation time, performing a quantitative real-time PCR (qPCR) method for VTG quantification, and verifying the model system with reference chemicals 17β-estradiol, estrone, diethylstilbestrol, hexestrol, genistein, and a negative control, corticosterone. To test the performance of both freshly isolated and cryopreserved hepatocytes, mRNA was collected from hepatocytes following 24 h treatment for VTG gene expression analysis, whereas cell culture media was collected for a VTG ELISA 96 h post-treatment. EC50 values were obtained for each reference chemical except for corticosterone, which exhibited no induction of VTG gene or protein level. Our results show linear concordance between ELISA and qPCR detection methods. Although there was approximately 50% reduction in VTG inducibility following cryopreservation, linear concordance of EC50 values was found between freshly isolated and cryopreserved hepatocytes, indicating that cryopreservation does not alter the functional assessment of estrogen receptor activation and therefore VTG expression. These studies demonstrate that qPCR is a sensitive and specific method for detecting VTG gene expression that can be used together

  16. Isolation of total ribonucleic acid from fresh and frozen-thawed boar ...

    African Journals Online (AJOL)

    RNA) from raw fresh semen and frozen-thawed boar semen, using a protocol comprising the conventional TRIzol assay and a membrane-based technique, the PureLink RNA mini kit. Bioanalyzer profile revealed that the sperm RNA size ...

  17. Fate of challenge schistosomula in the murine anti-schistosome vaccine model

    International Nuclear Information System (INIS)

    Von Lichtenberg, F.; Correa-Oliveira, R.; Sher, A.

    1985-01-01

    Mice exposed to irradiated cercariae of Schistosoma mansoni develop a partial resistance to subsequent parasite challenge. In this study the authors utilized histopathologic methods to investigate the fate of both the immunizing and challenge cercariae in C57BL/6J mice. After immunization by percutaneous infection, a large number of the 50 Kr irradiated organisms could be detected in tissue sections of lung. However, as early as 2 weeks after immunization, the majority of these schistosomula apparently had died, leaving residual inflammatory foci. The numbers of these foci then gradually declined during the next 4 weeks of examination. Cercarial challenge of mice vaccinated 4 weeks previously provoked an intense eosinophil-enriched inflammatory response in percutaneously exposed ear pinnae. Despite these pronounced tissue reactions, no evidence of significant parasite damage or attrition was detected in this migration site. In contrast, schistosomula arriving in the lungs of vaccinated mice produced a greater number of residual inflammatory foci than did larvae appearing in the lungs of normal mice. In addition, challenge schistosomula were cleared from the lungs of vaccinated mice at a slower rate than they were from the lungs of control mice. These observations suggest that the lung is a major site of parasite attrition for both immunizing and challenge infections in the mouse irradiated vaccine model

  18. Vaccination against bovine schistosomiasis japonica with highly X-irradiated schistosomula

    International Nuclear Information System (INIS)

    Hsue, S.Y.; Hsue, H.F.; Xu, S.T.; Shi, F.H.; He, Y.X.; Clarke, W.R.; Johnson, S.C.

    1983-01-01

    Cercariae of the Chinese mainland strain of Schistosoma japonicum were used. Eighteen cattle, divided into six groups of three each, were immunized with schistosomula transformed from cercariae exposed to three different doses of X-irradiation (24, 36, and 48 kR). The immunization was given either once, twice, or thrice, and the number of immunizing schistosomula was 10,000 or more in each immunization. The immunized cattle were challenged with 500 normal cercariae. Five native cattle were similarly infected with normal cercariae as controls. All cattle were killed 32-33 days after challenge or infection, and the worms were obtained by perfusion. The mean worm reduction in the 18 experimental animals varied from 42.1 to 96.0%. The mean percent worm reduction of the six experimental groups varied from 54.8 to 87.1. The reduction was greater with increasing numbers of immunizations, and was higher in the groups immunized with schistosomula exposed to 36 kR than in those exposed to 24 or 48 kR. Statistical analyses showed that all immunized groups yielded significantly fewer worms than controls. However, the three doses of X-irradiation (24, 36, and 48 kR) had no significant effect for a fixed number of immunizations (1, 2, or 3). The means for both two and three immunizations were significantly different from the mean for one immunization, although they were not significantly different from each other

  19. Cloning of a cDNA encoding a surface antigen of Schistosoma mansoni schistosomula recognized by sera of vassinated mice

    International Nuclear Information System (INIS)

    Dalton, J.P.; Tom, T.D.; Strand, M.

    1987-01-01

    Spleen cells of mice vaccinated with radiation-attenuated Schistosoma mansoni cercariae were used to produce monoclonal antibodies directed against newly transformed schistosomular surface antigens. One of these monoclonal antibodies recognized a polypeptide of 18 kDa. Binding was measured by radioimmunoassay. This glycoprotein was purified by monoclonal antibody immunoaffinity chromatography and a polyclonal antiserum was prepared against it. Immunofluorescence assays showed that the polyclonal antiserum bound to the surface of newly transformed schistosomula and lung-stage organisms but not to the surface of liver-stage and adult worms. Using this polyclonal antiserum we isolated recombinant clones from an adult worm cDNA expression library constructed in λgt11. Clone 654.2 contained an insert of 0.52 kilobase and hybridized to a 1.2-kilobase mRNA species from adult worms. Most importantly, clone 654.2 produced a fusion protein of 125 kDa that was reactive with sera of vaccinated mice that are capable of transferring resistance. This result encourages future vaccination trials with the fusion protein

  20. Short communication: Characterization of methicillin-resistant Staphylococcus aureus isolated from raw milk fresh cheese in Colombia.

    Science.gov (United States)

    Herrera, Fanny C; García-López, María-Luisa; Santos, Jesús A

    2016-10-01

    The aim of this study was the characterization of a collection of 8 methicillin-resistant Staphylococcus aureus (MRSA) isolates, obtained from samples of fresh cheese (Doble Crema) produced from raw cow milk in small dairies in Colombia. All the isolates harbored the mecA and Panton-Valentine leukocidin (PVL) genes, presented with SCCmec type IV, and belonged to multilocus sequence type 8 and spa type 024. Seven isolates presented 3 closely related pulsed-field gel electrophoresis profiles. Three of them carried the staphylococcal enterotoxin B gene. The isolates were resistant to cefoxitin, oxacillin, penicillin, and ampicillin and susceptible to all non-β-lactams antibiotics tested, with minimum inhibitory concentration values for oxacillin of 4 to 8mg/L. The isolates belonged to the community-acquired MRSA group, suggesting a human source of contamination. The risk of human infection by MRSA via contaminated foods is considered low, but contaminated food commodities can contribute to the worldwide dissemination of clones of community-acquired MRSA. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  1. Species Distribution and Prevalence of Putative Virulence Factors in Mesophilic Aeromonas spp. Isolated from Fresh Retail Sushi

    Directory of Open Access Journals (Sweden)

    Sunniva Hoel

    2017-05-01

    Full Text Available Aeromonas spp. are ubiquitous bacteria that have received increasing attention as human pathogens because of their widespread occurrence in food, especially seafood and vegetables. The aim of this work was to assess the species identity and phylogenetic relationship of 118 Aeromonas strains isolated from fresh retail sushi from three producers, and to characterize the isolates with respect to genetic and phenotypic virulence factors. We also evaluate the potential hazard associated with their presence in ready-to-eat seafood not subjected to heat treatment. Mesophilic Aeromonas salmonicida was most prevalent (74%, followed by A. bestiarum (9%, A. dhakensis (5%, A. caviae (5%, A. media (4%, A. hydrophila (2%, and A. piscicola (1%. All isolates were considered potentially pathogenic due to the high prevalence of genes encoding hemolysin (hlyA (99%, aerolysin (aerA (98%, cytotoxic enterotoxin (act (86%, heat-labile cytotonic enterotoxin (alt (99%, and heat-stable cytotonic enterotoxin (ast (31%. The shiga-like toxins 1 and 2 (stx-1 and stx-2 were not detected. Moreover, there was heterogeneity in toxin gene distribution among the isolates, and the combination of act/alt/hlyA/aerA was most commonly detected (63%. β-hemolysis was species-dependent and observed in 91% of the isolates. All A. media and A. caviae strains were non-hemolytic. For isolates belonging to this group, lack of hemolysis was possibly related to the absence of the act gene. Swimming motility, linked to adhesion and host invasion, occurred in 65% of the isolates. Partial sequencing of the gyrB gene demonstrated its suitability as a genetic marker for Aeromonas species identification and for assessment of the phylogenetic relationship between the isolates. The gyrB sequence divergence within a given species ranged from 1.3 to 2.9%. A. bestiarum, A. salmonicida, and A. piscicola were the most closely related species; their sequences differed by 2.7–3.4%. The average gyrB sequence

  2. Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese

    Directory of Open Access Journals (Sweden)

    Ksenija Uroić

    2014-01-01

    Full Text Available The aim of this study is to gain insight into the probiotic potential of autochthonous lactic acid bacteria (LAB isolated from artisanal fresh soft and white pickled cheeses. Eleven out of 86 LAB isolates from traditionally produced artisanal fresh soft and white pickled cheeses which survived the most rigorous simulated gastrointestinal tract conditions and did not show resistance to antibiotics were subjected to further evaluation for functional probiotic properties. The ability of the examined strains to assimilate cholesterol in the presence of bile salts was strain dependent, with the highest percentage of cholesterol assimilated by strain Lactobacillus brevis BGGO7-28 possessing S-layer proteins on its cell surface. The growth of strains with mannitol or lactulose as the only carbon source was better than with fructooligosaccharides (FOS and inulin as prebiotic substrates, which should be considered in the production of synbiotics. Moreover, the results demonstrated that the strains were highly adhesive to human enterocyte-like Caco-2 cells and to a lesser extent to HT29-MTX cells, with the exception of strain Lb. brevis BGGO7-28, which showed similar percentage of adhesion to both cell lines. This strain was the only one with the acidic cell surface, while other examined strains have the cell surfaces with electron donor and basic properties. In addition, all selected strains decreased the proliferation of gut-associated lymphoid tissue (GALT cells, suggesting possible immunomodulatory potential of the isolates. Finally, the number of viable cells in dry active preparations after lyophilisation depended on the lyoprotectant used (inulin, FOS or skimmed milk, as well as on the strain subjected to lyophilisation. In conclusion, the results obtained in this study demonstrate that particular dairy LAB isolates exhibit strain-specific probiotic properties. Thus, they could be further examined as part of mixed autochthonous starter cultures for

  3. Site requirements and kinetics of immune-dependent elimination of intravascularly administered lung stage schistosomula in mice immunized with highly irradiated cercariae of Schistosoma mansoni

    International Nuclear Information System (INIS)

    Mangold, B.L.; Dean, D.A.; Coulson, P.S.; Wilson, R.A.

    1986-01-01

    Experiments were performed to compare the migration and survival of 75Se-labeled schistosomes, introduced by percutaneous cercarial exposure or by intravascular administration of 7-day-old lung stage schistosomula, in control and irradiated cercaria-immunized mice. Schistosomula were intravascularly introduced into the lungs, systemic organs and liver by injection via the femoral vein (FV), left ventricle (LV), and superior mesenteric vein (SMV), respectively. The fate of challenge larvae was examined by autoradiography of host tissues and by recovery of adult worms. It was found that both normal and immune elimination were site-dependent. In control mice 45%-60% of cercarial penetrants and lung schistosomula injected into the FV and LV were recoverable as adult worms, while a significantly greater number (70%-85%) were recoverable when lung schistosomula were injected into the SMV. In immunized mice, parasites introduced as either cercariae or FV-injected schistosomula were both highly sensitive to immune elimination. LV-injected schistosomula were also sensitive but to a slightly lesser degree. In contrast, schistosomula placed directly in the liver by SMV injection were totally insensitive to immune elimination. It was concluded that elimination of schistosomula in irradiated cercaria-immunized mice occurs in the lungs and/or in the systemic organs, but not in the liver. Also, it was concluded that immune elimination is not a rapid process, since more than 7 days were required after intravascular challenge for the development of demonstrable differences between control and immunized mice

  4. Phenotypic and Genetic Diversity of Aeromonas Species Isolated from Fresh Water Lakes in Malaysia.

    Directory of Open Access Journals (Sweden)

    Wei Ching Khor

    Full Text Available Gram-negative bacilli of the genus Aeromonas are primarily inhabitants of the aquatic environment. Humans acquire this organism from a wide range of food and water sources as well as during aquatic recreational activities. In the present study, the diversity and distribution of Aeromonas species from freshwater lakes in Malaysia was investigated using glycerophospholipid-cholesterol acyltransferase (GCAT and RNA polymerase sigma-factor (rpoD genes for speciation. A total of 122 possible Aeromonas strains were isolated and confirmed to genus level using the API20E system. The clonality of the isolates was investigated using ERIC-PCR and 20 duplicate isolates were excluded from the study. The specific GCAT-PCR identified all isolates as belonging to the genus Aeromonas, in agreement with the biochemical identification. A phylogenetic tree was constructed using the rpoD gene sequence and all 102 isolates were identified as: A. veronii 43%, A. jandaei 37%, A. hydrophila 6%, A. caviae 4%, A. salmonicida 2%, A. media 2%, A. allosaccharophila 1%, A. dhakensis 1% and Aeromonas spp. 4%. Twelve virulence genes were present in the following proportions--exu 96%, ser 93%, aer 87%, fla 83%, enolase 70%, ela 62%, act 54%, aexT 33%, lip 16%, dam 16%, alt 8% and ast 4%, and at least 2 of these genes were present in all 102 strains. The ascV, aexU and hlyA genes were not detected among the isolates. A. hydrophila was the main species containing virulence genes alt and ast either present alone or in combination. It is possible that different mechanisms may be used by each genospecies to demonstrate virulence. In summary, with the use of GCAT and rpoD genes, unambiguous identification of Aeromonas species is possible and provides valuable data on the phylogenetic diversity of the organism.

  5. Uptake of macromolecules by cercariae during skin penetration and transformation to schistosomula (Schistosoma mansoni

    Directory of Open Access Journals (Sweden)

    Joyce Thornhill

    2010-07-01

    Full Text Available Here, we observed the uptake of membrane-impermeant molecules by cercariae as they penetrate the skin and are transformed into schistosomula. We propose that membrane-impermeant molecules, Lucifer Yellow, Propidium iodide and Hoechst 33258 enter the parasite through both thenephridiopore and the surface membrane and then diffuse throughout the body of the parasite. We present a hypothesis that the internal cells of the body of the schistosomulum represent a new host-parasite interface, at which skin-derived growth factors may stimulate receptors on internal membranes during transformation of the cercariae into the schistosomulum.

  6. Isolation and characterisation of an enterocin P-producing Enterococcus lactis strain from a fresh shrimp (Penaeus vannamei).

    Science.gov (United States)

    Ben Braïek, Olfa; Ghomrassi, Hamdi; Cremonesi, Paola; Morandi, Stefano; Fleury, Yannick; Le Chevalier, Patrick; Hani, Khaled; Bel Hadj, Omrane; Ghrairi, Taoufik

    2017-06-01

    Screening for lactic acid bacteria (LAB) from fresh shrimp samples (Penaeus vannamei) collected from retail seafood markets in the Tunisian's coast, resulted in the isolation of an Enterococcus strain termed Q1. This strain was selected for its antagonistic activity against pathogenic bacteria such as Listeria monocytogenes, Pseudomonas aeruginosa, Lactococcus garvieae and against fungi (Aspergillus niger and Fusarium equiseti). The Q1 strain was characterised using standard morphological and biochemical tests, growth assays at different temperatures, pH and salinity. 16S rRNA, rpoA and pheS gene sequencing, as well as the 16S-23S rRNA intergenic spacer analyses, were combined to identify strain Q1 as a strain of Enterococcus lactis. The bacteriocin produced by E. lactis Q1 is thermostable, active in the pH range from 4.0 to 9.0 and has a bactericidal mode of action. The enterocin P structural gene was detected by specific PCR in strain E. lactis Q1, which is in good agreement with SDS-PAGE data of the purified bacteriocin. A lack of significant antibiotic resistance genes and virulence determinants was confirmed by specific PCRs. This work provides the first description of an enterocin P producer E. lactis strain isolated from a fresh shrimp. Based on its safety properties (absence of haemolytic activity, virulence factors and antibiotic resistance genes), this strain has the potential to be used as a natural additive or adjunct protective culture in food biopreservation and/or probiotic culture.

  7. Antischistosomal activity of a calcium channel antagonist on schistosomula and adult Schistosoma mansoni worms

    Directory of Open Access Journals (Sweden)

    Vanessa Silva-Moraes

    2013-08-01

    Full Text Available Current schistosomiasis control strategies are largely based on chemotherapeutic agents and a limited number of drugs are available today. Praziquantel (PZQ is the only drug currently used in schistosomiasis control programs. Unfortunately, this drug shows poor efficacy in patients during the earliest infection phases. The effects of PZQ appear to operate on the voltage-operated Ca2+channels, which are located on the external Schistosoma mansoni membrane. Because some Ca2+channels have dihydropyridine drug class (a class that includes nifedipine sensitivity, an in vitro analysis using a calcium channel antagonist (clinically used for cardiovascular hypertension was performed to determine the antischistosomal effects of nifedipine on schistosomula and adult worm cultures. Nifedipine demonstrated antischistosomal activity against schistosomula and significantly reduced viability at all of the concentrations used alone or in combination with PZQ. In contrast, PZQ did not show significant efficacy when used alone. Adult worms were also affected by nifedipine after a 24 h incubation and exhibited impaired motility, several lesions on the tegument and intense contractility. These data support the idea of Ca2+channels subunits as drug targets and favour alternative therapeutic schemes when drug resistance has been reported. In this paper, strong arguments encouraging drug research are presented, with a focus on exploring schistosomal Ca2+channels.

  8. Production of anatoxin-a by cyanobacterial strains isolated from Portuguese fresh water systems.

    Science.gov (United States)

    Osswald, Joana; Rellán, Sandra; Gago-Martinez, Ana; Vasconcelos, Vítor

    2009-11-01

    The occurrence of anatoxin-a in several freshwater systems in Portugal and its production by Portuguese cyanobacterial strains, after cultivation in laboratory, were studied. Surface water samples from 9 water bodies, for recreational and human consumption usage, were surveyed for anatoxin-a presence and for obtaining cultures of pure cyanobacterial strains. Anatoxin-a analysis was performed by high performance liquid chromatography (HPLC) with fluorescence detection (FLD) followed by Mass Spectrometry (MS) confirmation. No anatoxin-a was detected in all the natural water samples (limit of detection (LOD) = 25 ng l(-1)) but among the 22 isolated cyanobacterial strains, 13 could produce anatoxin-a in laboratory conditions (LOD = 3 ng g(-1) dw). This proportion of anatoxin-a producing strains (59.1%) in laboratory is discussed considering the hypothesis that anatoxin-a is a more frequent metabolite in cyanobacteria than it was thought before and making its occurrence in Portuguese freshwaters almost certain. Therefore, health and ecological risks caused by anatoxin-a in Portugal, should be seriously considered.

  9. Floricoccus tropicus gen. nov., sp. nov. and Floricoccus penangensis sp. nov. isolated from fresh flowers of durian tree and hibiscus.

    Science.gov (United States)

    Chuah, Li-Oon; Yap, Kien-Pong; Shamila-Syuhada, Ahamed Kamal; Thong, Kwai Lin; Ahmad, Rosma; Liong, Min Tze; Rusul, Gulam

    2017-12-01

    Three strains of Gram-staining-positive, coccus-shaped, lactic acid bacteria, designated as HibF3 T , HibF2 and HibF5 were isolated from fresh flowers of hibiscus, and a fourth, DF1 T , was isolated from fresh flowers of durian tree, in Penang, Malaysia. Taxonomic characterisation was performed by polyphasic analysis. Sequence similarities of the 16S rRNA gene and the housekeeping rpoA and pheS genes of these strains with their closely-related lactococcal and streptococcal relatives were 92-94, 78 and 81 %, respectively. The results of phylogenetic analysis indicated that strains DF1 T , HibF2, HibF5 and HibF3 T were clustered together but were clearly separated from species of the genera Streptococcus and Lactococcus, indicating that they represent members of a novel genus of the family Streptococcaceae. Calculation of average nucleotide identity (ANI) values between the genomes of DF1 T and HibF3 T yielded values of 92.50-92.93 %. ANI values below the cut-off value and distinctive chemotaxonomic characteristics supported the hypothesis that these strains represented two novel species. Major cellular fatty acids in DF1 T , HibF2 and HibF5 were C18 : 1ω7c and C16 : 0, while C12 : 0 and C14 : 0 were also dominant, in addition to C18 : 1ω7c and C16 : 0, in HibF3 T . A novel genus is proposed with the name Floricoccus gen. nov. which consists of two species, Floricoccus tropicus sp. nov as the type species, and Floricoccus penangensis sp. nov. The respective type strains are DF1 T (=LMG 29833 T =JCM 31733 T ) and HibF3 T (=LMG 29831 T =DSM 31735 T ).

  10. Characterization of Listeria monocytogenes isolated from a fresh mixed sausage processing line in Pelotas-RS by PFGE

    Science.gov (United States)

    von Laer, Ana Eucares; de Lima, Andréia Saldanha; Trindade, Paula dos Santos; Andriguetto, Cristiano; Destro, Maria Teresa; da Silva, Wladimir Padilha

    2009-01-01

    Listeria monocytogenes is a bacterium capable to adhere to the surfaces of equipment and utensils and subsequently form biofilms. It can to persist in the food processing environmental for extended periods of time being able to contaminate the final product. The aim of this study was to trace the contamination route of L. monocytogenes on a fresh mixed sausage processing line, from raw material to the final product. The isolates obtained were characterized by serotyping and molecular typing by pulsed-field gel electrophoresis (PFGE) using the restriction enzymes ApaI and AscI. L. monocytogenes was detected in 25% of the samples. The samples of raw material were not contaminated, however, the microorganism was detected in 21% of the environmental samples (food contact and non-food contact), 20.8% of the equipments, 20% of the food worker’s hands, 40% of the mass ready to packaging and in all the final products samples, demonstrating that the contamination of final product occurred during the processing and the importance of cross contamination. PFGE yielded 22 pulsotypes wich formed 7 clusters, and serotyping yielded 3 serotypes and 1 serogroup, however, the presence of serotypes 4b and 1/2b in the final product is of great concern for public health. The tracing of contamination showed that some strains are adapted and persisted in the processing environment in this industry. PMID:24031402

  11. Partial protection of baboons against Schistosoma mansoni using radiation-attenuated cryopreserved schistosomula

    International Nuclear Information System (INIS)

    James, E.R.; Dobinson, A.R.; Otieno, M.; Monorei, J.; Else, J.G.

    1986-01-01

    Three groups of five baboons were vaccinated in Kenya using three doses of 10,000 viable cryopreserved schistosomula attenuated with either 10, 20 or 60 krad 60 co-irradiation. The results from perfusion indicated reductions in worm burdens in the 10, 20 and 60 krad vaccinated groups of 18%, 23% and 20% respectively, none of which was statistically significant. No stunting of adult worms could be demonstrated in any of the groups. Mean tissue egg burdens were higher in all vaccinated groups and consequently egg production per worm pair was also higher than in the challenge controls. The logistics of preparing and delivering a cryopreserved radiation-attenuated vaccine were amply demonstrated; however, in this study the levels of protection achieved were not statistically significant; possible reasons for this are discussed. (author)

  12. Protection of sheep against Schistosoma bovis using cryopreserved radiation-attenuated schistosomula

    International Nuclear Information System (INIS)

    James, E.R.; Dobinson, A.R.; Andrews, B.J.; Bickle, Q.D.; Taylor, M.G.; Ham, P.J.

    1985-01-01

    Three sheep were vaccinated with two doses of 3 krad-irradiated cryopreserved Schistosoma bovis schistosomula containing 20,000 and 17,000 organisms respectively, injected intramuscularly 23 days apart after storage in liquid nitrogen for between 9 and 46 days. A challenge of 5360 S. bovis cercariae was administered percutaneously approximately four weeks after the last vaccine dose to these animals and to three controls. Post-challenge the vaccinated animals gained significantly more weight (27% v. 9%), produced fewer eggs in their faeces, showed a smaller reduction in PCV values (-18% v. -27%) and were over-all in better condition than control animals. At perfusion 49.1% fewer adult worms were found in the vaccinated sheep than in controls. The tissue egg burdens were similar in both groups. Histopathologically both groups were similar except that fewer and smaller egg lesions were observed in the livers of vaccinated animals. (author)

  13. Vaccination of bovines against Schistosomiasis japonica with highly irradiated schistosomula in China

    International Nuclear Information System (INIS)

    Hsue, S.Y.; Xu, S.T.; He, Y.X.; Shi, F.H.; Shen, W.; Hsue, H.F.; Osborne, J.W.; Clarke, W.R.

    1984-01-01

    Vaccination of Chinese bovines (cattle and buffaloes) against Schistosomiasis japonica with 36 kR gamma-irradiated schistosomula was done for laboratory challenge and for field trials in China. Altogether, 61 bovines were used. All experimental animals were vaccinated 2-3 times with 10,000 irradiated schistosomula per time. For the laboratory challenge, all experimental and control cattle were challenged with 500 normal cercariae and each buffalo, with 2,000 cercariae. The laboratory-challenged bovines were killed after 54-57 days of challenge; the bovines for the field trial in the lightly endemic area, after 5 months in the field; and the bovines for the field trial in the heavily endemic area, after 58-63 days. When the animals were killed, the number of mature worms in the vaccinated (experimental) and non-vaccinated (control) animals was recorded and the percentage of worm reduction in each group was calculated. The first group, consisting of three vaccinated and three non-vaccinated cattle, was given a laboratory challenge; the worm reduction was 71.6%. The second group, consisting of two vaccinated and three non-vaccinated buffaloes, was also given a laboratory challenge; the worm reduction was 74.4%. The third group, consisting of seven vaccinated and eight non-vaccinated buffaloes, was utilized in a field trial in a lightly endemic area; the worm reduction was 75.6%. The fourth group, consisting of eight vaccinated and nine non-vaccinated cattle, and the fifth group, consisting of nine vaccinated and nine non-vaccinated buffaloes, were pastured in a heavily endemic area. The worm reduction was 65.1% in the fourth group and 75.7% in the fifth group

  14. Protein kinase A and C regulate leak potassium currents in freshly isolated vascular myocytes from the aorta.

    Directory of Open Access Journals (Sweden)

    Sébastien Hayoz

    Full Text Available We tested the hypothesis that protein kinase A (PKA inhibits K2P currents activated by protein kinase C (PKC in freshly isolated aortic myocytes. PDBu, the PKC agonist, applied extracellularly, increased the amplitude of the K2P currents in the presence of the "cocktail" of K(+ channel blockers. Gö 6976 significantly reduced the increase of the K2P currents by PDBu suggesting the involvement of either α or β isoenzymes of PKC. We found that forskolin, or membrane permeable cAMP, did not inhibit K2P currents activated by the PKC. However, when PKA agonists were added prior to PDBu, they produced a strong decrease in the K2P current amplitudes activated by PKC. Inhibition of PDBu-elicited K2P currents by cAMP agonists was not prevented by the treatment of vascular smooth muscle cells with PKA antagonists (H-89 and Rp-cAMPs. Zn(2+ and Hg(2+ inhibited K2P currents in one population of cells, produced biphasic responses in another population, and increased the amplitude of the PDBu-elicited K(+ currents in a third population of myocytes, suggesting expression of several K2P channel types. We found that cAMP agonists inhibited biphasic responses and increase of amplitude of the PDBu-elicited K2P currents produced by Zn(2+ and Hg(2. 6-Bnz-cAMp produced a significantly altered pH sensitivity of PDBu-elicited K2P-currents, suggesting the inhibition of alkaline-activated K2P-currents. These results indicate that 6-Bnz-cAMP and other cAMP analogs may inhibit K2P currents through a PKA-independent mechanism. cAMP analogs may interact with unidentified proteins involved in K2P channel regulation. This novel cellular mechanism could provide insights into the interplay between PKC and PKA pathways that regulate vascular tone.

  15. Trifluoperazine inhibits acetaminophen-induced hepatotoxicity and hepatic reactive nitrogen formation in mice and in freshly isolated hepatocytes

    Directory of Open Access Journals (Sweden)

    Sudip Banerjee

    Full Text Available The hepatotoxicity of acetaminophen (APAP occurs by initial metabolism to N-acetyl-p-benzoquinone imine which depletes GSH and forms APAP-protein adducts. Subsequently, the reactive nitrogen species peroxynitrite is formed from nitric oxide (NO and superoxide leading to 3-nitrotyrosine in proteins. Toxicity occurs with inhibited mitochondrial function. We previously reported that in hepatocytes the nNOS (NOS1 inhibitor NANT inhibited APAP toxicity, reactive nitrogen and oxygen species formation, and mitochondrial dysfunction. In this work we examined the effect of trifluoperazine (TFP, a calmodulin antagonist that inhibits calcium induced nNOS activation, on APAP hepatotoxicity and reactive nitrogen formation in murine hepatocytes and in vivo. In freshly isolated hepatocytes TFP inhibited APAP induced toxicity, reactive nitrogen formation (NO, GSNO, and 3-nitrotyrosine in protein, reactive oxygen formation (superoxide, loss of mitochondrial membrane potential, decreased ATP production, decreased oxygen consumption rate, and increased NADH accumulation. TFP did not alter APAP induced GSH depletion in the hepatocytes or the formation of APAP protein adducts which indicated that reactive metabolite formation was not inhibited. Since we previously reported that TFP inhibits the hepatotoxicity of APAP in mice without altering hepatic APAP-protein adduct formation, we examined the APAP treated mouse livers for evidence of reactive nitrogen formation. 3-Nitrotyrosine in hepatic proteins and GSNO were significantly increased in APAP treated mouse livers and decreased in the livers of mice treated with APAP plus TFP. These data are consistent with a hypothesis that APAP hepatotoxicity occurs with altered calcium metabolism, activation of nNOS leading to increased reactive nitrogen formation, and mitochondrial dysfunction. Keywords: Acetaminophen, Neuronal nitric oxide, Oxidative stress, Mitochondria

  16. Comparison of Chemical Sensitivity of Fresh and Long-Stored Heat Resistant Neosartorya fischeri Environmental Isolates Using BIOLOG Phenotype MicroArray System.

    Directory of Open Access Journals (Sweden)

    Jacek Panek

    Full Text Available Spoilage of heat processed food and beverage by heat resistant fungi (HRF is a major problem for food industry in many countries. Neosartorya fischeri is the leading source of spoilage in thermally processed products. Its resistance to heat processing and toxigenicity makes studies about Neosartorya fischeri metabolism and chemical sensitivity essential. In this study chemical sensitivity of two environmental Neosartorya fischeri isolates were compared. One was isolated from canned apples in 1923 (DSM3700, the other from thermal processed strawberry product in 2012 (KC179765, used as long-stored and fresh isolate, respectively. The study was conducted using Biolog Phenotype MicroArray platforms of chemical sensitivity panel and traditional hole-plate method. The study allowed for obtaining data about Neosartorya fischeri growth inhibitors. The fresh isolate appeared to be much more resistant to chemical agents than the long-stored isolate. Based on phenotype microarray assay nitrogen compounds, toxic cations and membrane function compounds were the most effective in growth inhibition of N. fischeri isolates. According to the study zaragozic acid A, thallium(I acetate and sodium selenate were potent and promising N. fischeri oriented fungicides which was confirmed by both chemical sensitivity microplates panel and traditional hole-plate methods.

  17. Prevalence study of Vibrio species and frequency of the virulence genes of Vibrio parahaemolyticus isolated from fresh and salted shrimps in Genaveh seaport

    Directory of Open Access Journals (Sweden)

    S Hosseini

    2014-08-01

    Full Text Available Vibrio species are important seafood-borne pathogens that are responsible for 50-70% of gasteroenteritis. The present study was carried out in order to determine the prevalence of Vibrio species and the distribution of tdh, tlh and trh virulence genes in Vibrio parahaemolyticus isolated from fresh and salted shrimp samples. Totally, 60 fresh and salted shrimp samples were collected from the Genaveh seaport. Microbial culture was used to isolate Vibrio species. In addition, the presences of Vibrio parahaemolyticus, Vibrio cholera, Vibrio vulnificus and Vibrio harveyi and the virulence genes of V. parahaemolyticus were studied using the PCR method. Results showed that 20% of fresh and 23.33% of salted shrimp samples were positive for Vibrio species. In studied samples, V. vulnificus had the highest prevalence rate (8.33%, while V. cholera had the lowest prevalence rate (1.66%. From a total of 4 detected V. parahaemolyticus, all of them had tlh gene (100%. The distribution of tdh and trh genes in isolated V. parahaemolyticus strains were 50% and 25%, respectively. High prevalence of Vibrio species and especially virulent V. parahaemolyticus in samples confirmed the lack of hygienic condition in the production and distribution centers of shrimp.

  18. Isolation, characterization and identification of pericarp-degrading bacteria for the production of off-odour-free white pepper from fresh berries of Piper nigrum L.

    Science.gov (United States)

    Vinod, V; Kumar, A; Zachariah, T J

    2014-04-01

    To isolate, fermentatively evaluate and identify black pepper (Piper nigrum L.)-associated bacteria for the microbial decortication of fresh ripened berries and dried black pepper for preparation of off-odour-free white pepper. Among 45 bacterial isolates obtained from black pepper, seven of them were found to decorticate black pepper (>60%) and fresh pepper berries (98-100%) into white pepper within 5 days of immersion in bacterial suspension. The 16S rRNA genes (1500-bp amplicon) of these bacteria were sequenced, and species identity was established by closest match in GenBank. Superior-quality white pepper was obtained with Bacillus subtilis (IISR WP 33, 34, 38), Bacillus licheniformis (IISR WP 43), Acinetobacter baumanii (IISR WP 35), Klebsiella pneumoniae (IISR WP 19) and Microbacterium barkeri (IISR WP25). The bacterial isolates were found to secrete multiple hydrolytic enzymes such as cellulase, pectinase, amylase, protease and xylanase. Bacterial cultures were deposited with International Depository Authority at Microbial Type Culture Collection, India, as patent deposits as prescribed in Budapest Treaty for microbial deposits. The white pepper, thus obtained from bacterial decortication process, was free from off-odour compound, especially skatole. Other biochemical constituents such as oleoresin, piperine and essential oils were found in the acceptable range. The bacterial decortication did not affect inherent constituents of pepper such as essential oil constituents, oleoresin and piperine content. One of the most significant findings of the work is identification of specific bacterial species for decortication of fresh berries or black pepper berries into value-added white pepper. This work paved way for developing a technological process for microbial decortication of fresh/black pepper for the production of superior-quality white pepper. © 2014 The Society for Applied Microbiology.

  19. Schistosoma mansoni: parasitology and immunology of baboons vaccinated with irradiated cryopreserved schistosomula

    Energy Technology Data Exchange (ETDEWEB)

    Damian, R.T.; Powell, M.R.; Roberts, M.L. (Georgia Univ., Athens (USA). Dept. of Zoology); Clark, J.D. (Georgia Univ., Athens (USA). Lab. Animal Medicine); Stirewalt, M.A.; Lewis, F.A. (Biomedical Research Inst., Rockville, MD (USA))

    1985-06-01

    Young baboons (Papio cynocephalus) were vaccinated with ..gamma..-irradiated (500 Gy) cryopreserved Puerto Rican strain schistosomula of S. mansoni. Protection against heterologous, normal Kenyan Strain S. mansoni challenge infection was erratic and partial; and two putative correlates of immunity, reduced worm fecundity and change in worm location (anterior shift) were not observed. However, immunization of baboons with this vaccine resulted in a stimulated immune system. Both cellular and humoral anamnesis were demonstrable in vaccinated-challenged baboons. Schistosome infection-associated IgM hypergammaglobulinemia was also greatly reduced in vaccinated-challenged baboons. However IgG antibodies to adult, egg, and cercarial antigens were increased after challenge infection in preimmunized baboons. Vaccination appears to have resulted in a redirection of the immune system into anti-parasite channels, but this more specific immune response was insufficient to confer good protection against challenge infection in this experiment. The dampening effect of the vaccine on the hypergammaglobulinemia of schistosomiasis is another candidate for a possible ''anti-pathogenesis'' effect of irradiated schistosome larval vaccines.

  20. Schistosoma mansoni: parasitology and immunology of baboons vaccinated with irradiated cryopreserved schistosomula

    Energy Technology Data Exchange (ETDEWEB)

    Damian, R T; Powell, M R; Roberts, M L [Georgia Univ., Athens (USA). Dept. of Zoology; Clark, J D [Georgia Univ., Athens (USA). Lab. Animal Medicine; Stirewalt, M A; Lewis, F A [Biomedical Research Inst., Rockville, MD (USA)

    1985-06-01

    Young baboons (Papio cynocephalus) were vaccinated with ..gamma..-irradiated (500 Gy) cryopreserved Puerto Rican strain schistosomula of S. mansoni. Protection against heterologous, normal Kenyan Strain S. mansoni challenge infection was erratic and partial; and two putative correlates of immunity, reduced worm fecundity and change in worm location (anterior shift) were not observed. However, immunization of baboons with this vaccine resulted in a stimulated immune system. Both cellular and humoral anamnesis were demonstrable in vaccinated-challenged baboons. Schistosome infection-associated IgM hypergammaglobulinemia was also greatly reduced in vaccinated-challenged baboons. However IgG antibodies to adult, egg, and cercarial antigens were increased after challenge infection in preimmunized baboons. Vaccination appears to have resulted in a redirection of the immune system into anti-parasite channels, but this more specific immune response was insufficient to confer good protection against challenge infection in this experiment. The dampening effect of the vaccine on the hypergammaglobulinemia of schistosomiasis is another candidate for a possible ''anti-pathogenesis'' effect of irradiated schistosome larval vaccines.

  1. Schistosoma mansoni: parasitology and immunology of baboons vaccinated with irradiated cryopreserved schistosomula

    International Nuclear Information System (INIS)

    Damian, R.T.; Powell, M.R.; Roberts, M.L.

    1985-01-01

    Young baboons (Papio cynocephalus) were vaccinated with γ-irradiated (500 Gy) cryopreserved Puerto Rican strain schistosomula of S. mansoni. Protection against heterologous, normal Kenyan Strain S. mansoni challenge infection was erratic and partial; and two putative correlates of immunity, reduced worm fecundity and change in worm location (anterior shift) were not observed. However, immunization of baboons with this vaccine resulted in a stimulated immune system. Both cellular and humoral anamnesis were demonstrable in vaccinated-challenged baboons. Schistosome infection-associated IgM hypergammaglobulinemia was also greatly reduced in vaccinated-challenged baboons. However IgG antibodies to adult, egg, and cercarial antigens were increased after challenge infection in preimmunized baboons. Vaccination appears to have resulted in a redirection of the immune system into anti-parasite channels, but this more specific immune response was insufficient to confer good protection against challenge infection in this experiment. The dampening effect of the vaccine on the hypergammaglobulinemia of schistosomiasis is another candidate for a possible ''anti-pathogenesis'' effect of irradiated schistosome larval vaccines. (author)

  2. Immunization of baboons with attenuated schistosomula of Schistosoma haematobium: levels of protection induced by immunization with larvae irradiated with 20 and 60 krad

    International Nuclear Information System (INIS)

    Harrison, R.A.; Bickle, Q.D.; Sturrock, R.F.; Taylor, M.G.; Webbe, G.; Kiare, S.; James, E.R.; Andrews, B.J.

    1990-01-01

    The authors have demonstrated that baboons can be immunized with S. haemotobium schistosomula irradiated with 20 krad in a regimen that induces 90% protection. While this high level of protection has stimulated a discussion on the feasibility of a human volunteer trial (Von Lichtenberg, 1985), results of further studies particularly on (i) the pathogensis of immunization per se (Byram et al., 1989), (ii) the longevity of protection, and (iii) the protective efficacy of cryopreserved irradiated S. haemotobium schistosomula (R. Harrison et al., in preparation), prevent recommending this form of vaccination for human application. (author)

  3. Comparison of human platelet lysate alternatives using expired and freshly isolated platelet concentrates for adipose-derived stromal cell expansion.

    Science.gov (United States)

    Dessels, Carla; Durandt, Chrisna; Pepper, Michael S

    2018-03-19

    Pooled human platelet lysate (pHPL) has been used to expand adipose-derived stromal cells (ASCs) and can be formulated using fresh or expired buffy coats (BCs) which are then resuspended in either plasma or an additive solution. Not much is known about the effects that expired products and additive solutions have on ASC expansion, and the need for quality control and release criteria has been expressed. This pilot study compared proliferation, cell size, morphology and immunophenotype of ASCs expanded in the different pHPL alternatives versus foetal bovine serum (FBS). Quality control criteria were assessed prior to and during the manufacture of the pHPL alternatives. ASCs were then expanded in 1%, 2.5%, 5% or 10% of the different pHPL alternatives or in 10% FBS. Cell size, morphology, cell number and immunophenotype were measured using microscopy and flow cytometry. The majority of the pHPL alternatives were within the recommended ranges for the quality control criteria. ASCs expanded in the pHPL alternatives were smaller in size, displayed a tighter spindle-shaped morphology, increased cell growth and had a similar immunophenotype (with the exception of CD34 and CD36) when compared to ASCs expanded in FBS. Here we report on the effects that expired BC products and additive solutions have on ASC expansion. When taken together, our findings indicate that all of the pHPL alternatives can be considered to be suitable replacements for FBS for ASC expansion, and that expired BC products can be used as an alternative to fresh BC products.

  4. Evaluation of toxic/protective effects of the essential oil of Salvia officinalis on freshly isolated rat hepatocytes

    OpenAIRE

    Lima, Cristóvão F.; Carvalho, Felix; Fernandes, Eduarda; Bastos, Maria de Lurdes; Gomes, P. Santos; Ferreira, Manuel Fernandes; Wilson, Cristina Pereira

    2004-01-01

    For this study the essential oil (EO) of sage (Salvia officinalis L.) was isolated from air-dried vegetative aerial parts of the plants by hydrodistillation and analysed by GC and GC-MS. A total yield of 12.07 mg of EO per g of plant dry mass was obtained and more than 50 compounds identified. The major compounds were cis-thujone (17.4 %), alpha-humulene (13.3 %), 1,8-cineole (12.7 %), E-caryophyllene (8.5 %) and borneol (8.3 %). The EO fraction of sage tea was also isolated by partition with...

  5. Specific receptors for phorbol diesters on freshly isolated human myeloid and lymphoid leukemia cells: comparable binding characteristics despite different cellular responses.

    Science.gov (United States)

    Goodwin, B J; Moore, J O; Weinberg, J B

    1984-02-01

    Freshly isolated human leukemia cells have been shown in the past to display varying in vitro responses to phorbol diesters, depending on their cell type. Specific receptors for the phorbol diesters have been demonstrated on numerous different cells. This study was designed to characterize the receptors for phorbol diesters on leukemia cells freshly isolated from patients with different kinds of leukemia and to determine if differences in binding characteristics for tritium-labeled phorbol 12,13-dibutyrate (3H-PDBu) accounted for the different cellular responses elicited in vitro by phorbol diesters. Cells from 26 patients with different kinds of leukemia were studied. PDBu or phorbol 12-myristate 13-acetate (PMA) caused cells from patients with acute myeloblastic leukemia (AML), acute promyelocytic (APML), acute myelomonocytic (AMML), acute monocytic (AMoL), acute erythroleukemia (AEL), chronic myelocytic leukemia (CML) in blast crisis (myeloid), acute undifferentiated leukemia (AUL), and hairy cell leukemia (HCL) (n = 15) to adhere to plastic and spread. However, they caused no adherence or spreading and only slight aggregation of cells from patients with acute lymphocytic leukemia (ALL), chronic lymphocytic leukemia (CLL), or CML-blast crisis (lymphoid) (n = 11). All leukemia cells studied, irrespective of cellular type, displayed specific receptors for 3H-PDBu. The time courses for binding by all leukemia types were similar, with peak binding at 5-10 min at 37 degrees C and 120 min at 4 degrees C. The binding affinities were similar for patients with ALL (96 +/- 32 nM, n = 4), CLL (126 +/- 32 nM, n = 6), and acute nonlymphoid leukemia (73 +/- 14 nM, n = 11). Likewise, the numbers of specific binding sites/cell were comparable for the patients with ALL (6.2 +/- 1.3 X 10(5) sites/cell, n = 4), CLL (5.0 +/- 2.0 X 10(5) sites/cell, n = 6), and acute nonlymphoid leukemia (4.4 +/- 1.9 X 10(5) sites/cell, n = 11). Thus, the differing responses to phorbol diesters of

  6. Uptake of ascorbic acid by freshly isolated cells and secretory granules from the intermediate lobe of ox hypophyses

    DEFF Research Database (Denmark)

    Zhou, A; Matsumoto, T; Farver, O

    1990-01-01

    Mechanically isolated cells from the intermediate lobe of ox hypophyses contained 40.6 +/- 3.7 nmol mg-1 protein (mean +/- SE, n = 5) of ascorbic acid. They accumulated radioactivity time dependently, on incubation with L-[14C]ascorbic acid in ionic medium dominated by NaCl. No definite saturation...

  7. Bacteriocinogenic Lactococcus lactis subsp. lactis DF04Mi isolated from goat milk: Application in the control of Listeria monocytogenes in fresh Minas-type goat cheese

    Directory of Open Access Journals (Sweden)

    Danielle N. Furtado

    2015-03-01

    Full Text Available Listeria monocytogenes is a pathogen frequently found in dairy products. Its control in fresh cheeses is difficult, due to the psychrotrophic properties and salt tolerance. Bacteriocinogenic lactic acid bacteria (LAB with proven in vitro antilisterial activity can be an innovative technological approach but their application needs to be evaluated by means of in situ tests. In this study, a novel bacteriocinogenic Lactococcus lactis strain (Lc. lactis DF4Mi, isolated from raw goat milk, was tested for control of growth of L. monocytogenes in artificially contaminated fresh Minas type goat cheese during storage under refrigeration. A bacteriostatic effect was achieved, and counts after 10 days were 3 log lower than in control cheeses with no added LAB. However, this effect did not differ significantly from that obtained with a non-bacteriocinogenic Lc. lactis strain. Addition of nisin (12.5 mg/kg caused a rapid decrease in the number of viable L. monocytogenes in the cheeses, suggesting that further studies with the purified bacteriocin DF4Mi may open new possibilities for this strain as biopreservative in dairy products.

  8. Feeling Fresh

    Science.gov (United States)

    ... Staying Safe Videos for Educators Search English Español Feeling Fresh KidsHealth / For Teens / Feeling Fresh Print en español La higiene femenina As ... the other products that claim to make women feel cleaner and fresher. But do these work? And ...

  9. Effect of whey protein isolate films incorporated with montmorillonite and citric acid on the preservation of fresh-cut apples.

    Science.gov (United States)

    Azevedo, Viviane Machado; Dias, Marali Vilela; de Siqueira Elias, Heloisa Helena; Fukushima, Katia Lumi; Silva, Eric Keven; de Deus Souza Carneiro, João; de Fátima Ferreira Soares, Nilda; Borges, Soraia Vilela

    2018-05-01

    The objective of this paper was to evaluate the effect of bioactive whey protein isolate/montmorillonite films containing citric acid on the inhibition of enzymatic browning and physicochemical properties in minimally processed apples. Whey protein isolate films incorporated with montmorillonite (3 g/100 g) and citric acid (5 and 10 g/100 g) were applied to the apples slices. All samples were packaged in polypropylene trays (14.6 cm × 11.4 cm × 6.5 cm) and stored at 5 ± 2 °C and 85 ± 3% RH for eight days. Every two days, the apples samples were evaluated for color, acidity, pH, soluble solids, water activity and polyphenol oxidase and peroxidase enzyme activity. The enzymatic browning of the apples slices was reduced for all films during storage. However, the films containing citric acid maintained the color characteristics, reducing the loss of quality associated the maintenance of acidity, soluble solids, water activity, reduction of polyphenol oxidase and peroxidase activity, thus prolonging the shelf life of the apples. Copyright © 2018 Elsevier Ltd. All rights reserved.

  10. Transcription of the var genes from a freshly-obtained field isolate of Plasmodium falciparum shows more variable switching patterns than long laboratory-adapted isolates.

    Science.gov (United States)

    Ye, Run; Zhang, Dongmei; Chen, Biaobang; Zhu, Yongqiang; Zhang, Yilong; Wang, Shengyue; Pan, Weiqing

    2015-02-07

    Antigenic variation in Plasmodium falciparum involves switching among multicopy var gene family and is responsible for immune evasion and the maintenance of chronic infections. Current understanding of var gene expression and switching patterns comes from experiments conducted on long laboratory-adapted strains, with little known about their wild counterparts. Genome sequencing was used to obtain 50 var genes from a parasite isolated from the China-Myanmar border. Four clones with different dominant var genes were cultured in vitro in replicates for 50 generations. Transcription of the individual var gene was detected by real-time PCR and then the switching process was analysed. The expression of multicopy var genes is mutually exclusive in clones of a wild P. falciparum isolate. The activation of distinct primary dominant var genes leads to different and favoured switching patterns in the four clones. The on/off rates of individual var genes are variable and the choice of subsequent dominant var genes are random, which results in the different switching patterns among replicates of each clonal wild P. falciparum isolate with near identical initial transcription profiles. This study suggests that the switching patterns of var genes are abundant, which consist of both conserved and random parts.

  11. Characterization of LDL-receptors of freshly isolated mononuclear cells of healthy subjects and of FH-patients

    International Nuclear Information System (INIS)

    Banyai, M.

    1991-05-01

    The central role of the LDL (=low density lipoproteins) receptor in artherosclerosis was first appreciated when it was shown that its absence was responsible for FH (familial hypercholesterolemia). To determine the high affinity cell surface binding activity in circulating human mononuclear cells (MNCs), these cells were incubated with low concentrations (1-50 μg protein/ml) of 123 I-LDL or 111 In-LDL either in the presence or absence of an excess of unlabeled LDL at 4 deg C for 45 minutes. MNCs of healthy subjects and of heterozygous FH-patients were found to possess high affinity LDL receptors immediately after they were isolated from the blood stream. The results indicate that the FH-patients enclosed in this study possess a reduced number of the same high affinity binding sites as healthy subjects confirming the diagnosis of heterozygous FH. In this study 123 I-LDL binding and 111 In-LDL binding to MNCs has been shown to saturable, reversible and displaceable and time-dependent. 123 I-LDL and 111 In-LDL as well can be recommended for the in-vitro determination of LDL-receptor binding activity as both binding processes show approximately the same characteristics. (author)

  12. ASIC-like currents in freshly isolated cerebral artery smooth muscle cells are inhibited by endogenous oxidase activity.

    Science.gov (United States)

    Chung, Wen-Shuo; Farley, Jerry M; Drummond, Heather A

    2011-01-01

    The aim of this study was to determine if VSMC ASIC-like currents are regulated by oxidative state. We used whole-cell patch clamp of isolated mouse cerebral VSMCs to determine if 1) reducing agents, such as DTT and GSH, and 2) inhibition of endogenous oxidase activity from NADPH and Xanthine oxidases potentiate active currents and activate electrically silent currents. Pretreatment with 2 mM DTT or GSH, increased the mean peak amplitude of ASIC-like currents evoked by pH 6.0 from 0.4 ± 0.1 to 14.9 ± 3.6 pA/pF, and from 0.9 ± 0.3 to 11.3 ± 2.4 pA/pF, respectively. Pretreatment with apocynin, a NADPH oxidase inhibitor, mimics the effect of the reducing agents, with the mean peak current amplitude increased from 0.9 ± 0.5 to 7.0 ± 2.6 pA/pF and from 0.5 ± 0.2 to 26.4 ± 6.8 pA/pF by 50 and 200 μM apocynin, respectively. Pretreatment with allopurinol, a xanthine oxidase inhibitor, also potentiates the VSMC ASIC-like activity. These findings suggest that VSMC ASIC-like channels are regulated by oxidative state and may be inhibited by basal endogenous oxidative sources such as NADPH and xanthine oxidase. Copyright © 2011 S. Karger AG, Basel.

  13. Polarized Th1 and Th2 cells are less responsive to negative feedback by receptors coupled to the AC/cAMP system compared to freshly isolated T cells

    NARCIS (Netherlands)

    Heijink, Irene H; Vellenga, Edo; Borger, Peter; Postma, Dirkje S; Monchy, Jan G R de; Kauffman, Henk F

    1 The adenylyl cyclase (AC)/cyclic adenosine monophosphate (cAMP) system is known to negatively regulate transcriptional activity of T cells, thereby possibly modulating T-cell-mediated responses at the sites of inflammation. Effects of cAMP have been widely studied in freshly isolated T cells and

  14. Studies on the surface antigenicity and susceptibility to antibody-dependent killing of developing schistosomula using sera from chronically infected mice and mice vaccinated with irradiated cercariae

    International Nuclear Information System (INIS)

    Bickle, Q.D.; Ford, M.J.

    1982-01-01

    Changes in the surface antigenicity and susceptibility to in vitro killing during development of schistosomula of Schistosoma mansoni were studied using serum from chronically infected mice (CIS) and from mice vaccinated with highly irradiated (20 krad) cercariae (VS). Binding of these sera was quantitated by counting the number of P388D 1 cells (a transformed, macrophage-like cell of mouse origin, bearing Fc receptors for IgG) binding to the parasite surface. Compared with schistosomula derived in vitro by mechanical transformation (MS), schistosomula recovered 3 hr after skin penetration in vitro (SS) showed a significant loss in surface binding of CIS. Schistosomula recovered 3 hr after skin penetration in vivo (SRS) showed even less binding, and this trend continued such that parasites recovered from the lungs 5 days after infection (LS) showed only minimal binding, and 10-day-old worms from the portal system showed no significant binding. In contrast, VS, which bound significantly less well to MS than CIS, showed enhanced binding to SS, and in the face of their declining antigenicity with respect to CIS, 3- to 24-hr SRS maintained this raised level of antigenicity. Although there appeared to be a decline in binding of VS thereafter, LS remained antigenic, still binding as many cells as MS did despite the fact that they also expressed host antigens detected usng antisera raised against mouse RBC. In spite of this persistence of VS binding up to the lung stage, resistance to eosinophil-mediated killing in vitro had developed by 48 hr post-infection, and LS were totally resistant to both eosinophil- and C-mediated killing

  15. An ultrastructural examination of irradiated, immunizing schistosomula of Schistosoma mansoni during their extended stay in the lungs

    International Nuclear Information System (INIS)

    Mastin, A.; Wilson, R.A.; Bickle, Q.D.

    1985-01-01

    An ultrastructural study has been performed on radiation-attenuated parasites fixed in situ during their extended stay in the lungs. At day 7 post-infection all parasites were located within blood vessels. In contrast to normally migrating parasites, at days 13 and 21 post-infection the majority of radiation-attenuated parasites were lying free within alveoli. It appeared that radiation-attenuated parasites had undergone the normal developmental changes associated with the lung-stage of migration. No ultrastructural evidence was found to suggest why schistosomula failed to migrate further and instead burst into alveoli. No parasites observed on day 7 post-infection were associated with a host inflammatory reaction. At day 13, inflammation was noticeable but could have been a response as much to pulmonary tissue damage as to the presence of parasites. At day 21 post-infection some parasites were internally disrupted but were not associated with host inflammatory reactions and had intact tegumental surfaces. One parasite was observed at day 21 with an adherent neutrophil infiltrate and extensive tegumental damage. The relevance of persisting lung-stage parasites to the induction of resistance to challenge is discussed. (author)

  16. Generation and functional analysis of T cell lines and clones specific for schistosomula released products (SRP-A).

    Science.gov (United States)

    Damonneville, M; Velge, F; Verwaerde, C; Pestel, J; Auriault, C; Capron, A

    1987-01-01

    Antigens present in the products released by the larval stage of schistosome (SRP-A) were shown to induce a strong cytotoxic and protective IgE response both in the rat and the monkey. T cell lines and clones specific for SRP-A or 26 kD antigens which are the main target of the cytotoxic IgE have been derived. The passive transfer of SRP-A specific T lymphocytes into infected rats led to an increase of the IgE response, conferring a significant level of protection to the rats. In coculture assays in vitro, these cell lines significantly enhanced the production of IgE by SRP-A sensitized rat spleen cells. This helper effect on the IgE response was confirmed with 26 kD T cell clone supernatants. Moreover, supernatants obtained after stimulation with phorbol myristate acetate were able to enhance the IgE production of a hybridoma B cell line (B48-14) producing a monoclonal IgE antibody, cytotoxic for the schistosomula. PMID:3498590

  17. Generation and functional analysis of T cell lines and clones specific for schistosomula released products (SRP-A).

    Science.gov (United States)

    Damonneville, M; Velge, F; Verwaerde, C; Pestel, J; Auriault, C; Capron, A

    1987-08-01

    Antigens present in the products released by the larval stage of schistosome (SRP-A) were shown to induce a strong cytotoxic and protective IgE response both in the rat and the monkey. T cell lines and clones specific for SRP-A or 26 kD antigens which are the main target of the cytotoxic IgE have been derived. The passive transfer of SRP-A specific T lymphocytes into infected rats led to an increase of the IgE response, conferring a significant level of protection to the rats. In coculture assays in vitro, these cell lines significantly enhanced the production of IgE by SRP-A sensitized rat spleen cells. This helper effect on the IgE response was confirmed with 26 kD T cell clone supernatants. Moreover, supernatants obtained after stimulation with phorbol myristate acetate were able to enhance the IgE production of a hybridoma B cell line (B48-14) producing a monoclonal IgE antibody, cytotoxic for the schistosomula.

  18. Comparison of the protective resistance induced by 60Co-irradiated cercariae and schistosomula of the WFFS and NMRI strains of Schistosoma mansoni

    International Nuclear Information System (INIS)

    James, E.R.; Dobinson, A.R.

    1985-01-01

    Mice, CBA/HT6T6 and C57BL/10, were vaccinated with 1 x 350 or 1 x 500 Schistosoma mansoni cercariae or schistosomula attenuated with 20 or 56 krad 60 Co irradiation and challenged with 200 cercariae. Protective resistance against homologous strain challenge was compared using the Winches Farm Field Station (WFFS) and Naval Medical Research Institute (NMRI) strains of S. mansoni. Maximal resistance to challenge was obtained in both strains of mice with cercariae or schistosomula of either WFFS or NMRI strain attenuated with 20 krad. Protection using organisms attenuated with 56 krad was significantly lower. Since previous studies with the two parasite strains have shown that the biological effects of irradiation are similar, the difference in the immunogenicity of the 56 krad-irradiated NMRI strain in this study from earlier studies must be due either to different local conditions for irradiation or to adaptation of the NMRI strain to a new laboratory environment. This finding may have important implications for vaccination studies and investigations of the mechanisms of immunity where radiation-attenuated parasites are used. (author)

  19. Feasibility of measuring radiation-induced DNA double strand breaks and their repair by pulsed field gel electrophoresis in freshly isolated cells from the mouse RIF-1 tumor

    International Nuclear Information System (INIS)

    Waarde, Maria A.W.H. van; Assen, Annette J. van; Konings, Antonius W.T.; Kampinga, Harm H.

    1996-01-01

    Purpose: To examine the technical feasibility of pulsed field gel electrophoresis (PFGE) as a predictive assay for the radio responsiveness of tumors. Induction and repair of DNA double strand breaks (DSBs) in a freshly prepared cell suspension from a RIF-1 tumor (irradiated ex vivo) was compared with DSB induction and repair in exponentially growing RIF-1 cells in culture (irradiated in vitro). Methods and Materials: A murine RIF-1 tumor grown in vivo was digested, and cells were exposed to x-rays (ex vivo) at doses of 1 to 75 Gy. DNA damage was measured using CHEF (clamped homogeneous electric fields) electrophoresis. Repair kinetics were studied at 37 deg. C for 4 h after irradiation. Radiosensitivity was determined by clonogenic assay, and cell cycle distributions by flow cytometry. For comparison, a trypsinized suspension of exponentially growing RIF-1 cells in vitro was run parallel with each ex vivo experiment. Results: Induction of DSBs, expressed as % DNA extracted from the plug, was similar in the in vitro and ex vivo irradiated cells. Compared to repair rates in in vitro cultured RIF-1 cells, repair kinetics in a freshly prepared cell suspension from the tumor were decreased, unrelated to differences in radiosensitivity. Differences in repair could not be explained by endogenous DNA degradation, nor by influences of enzymes used for digestion of the tumor. A lower plating efficiency and differences in ploidy (as revealed by flow cytometry) were the only reproducible differences between in vivo and in vitro grown cells that may explain the differences in repair kinetics. Conclusions: The current results do not support the idea that PFGE is a technique robust enough to be a predictive assay for the radiosensitivity of tumor cells

  20. Isolation and characterization of starch from industrial fresh pasta by-product and its potential use in sugar-snap cookie making.

    Science.gov (United States)

    Ellouzi, Soumaya Zouari; Driss, Dorra; Maktouf, Sameh; Neifar, Mohamed; Kobbi, Ameni; Kamoun, Hounaida; Chaabouni, Semia Ellouze; Ghorbel, Raoudha Ellouze

    2015-09-01

    In this paper, starch was extracted from fresh pasta by-product (PS) and its chemical composition and physical and microscopic characteristics were determined. Commercial wheat starch (CS) was used as reference. In general, purity was similar between starches studied. However, others compounds such as protein, lipid and ash were significantly different. PS starch granules had large lenticular-shape (25-33 μm) and small spherical-shape (5-8 μm). The pH and color of PS starch were similar to those reported for CS starch. On the other hand, PS had higher water absorption capacity, viscosity and cooking stability than CS. The gelatinization temperature of PS was similar to that of CS (60 and 61 °C). At high temperature (90 °C) both starches had similar rheological behavior. The results achieved suggest that PS starch has potential for application in food systems requiring high processing temperatures such the manufacture of sugar snap cookie. The effects of PS starch addition on the dough making stage and the final cookie quality were analyzed. Improvements in dough cohesiveness (24 %) and springiness (10 %) were significant relative to those of CS dough. Texture profile analysis confirmed the rheological changes.

  1. Frozen cord blood hematopoietic stem cells differentiate into higher numbers of functional natural killer cells in vitro than mobilized hematopoietic stem cells or freshly isolated cord blood hematopoietic stem cells.

    Directory of Open Access Journals (Sweden)

    Martha Luevano

    Full Text Available Adoptive natural killer (NK cell therapy relies on the acquisition of large numbers of NK cells that are cytotoxic but not exhausted. NK cell differentiation from hematopoietic stem cells (HSC has become an alluring option for NK cell therapy, with umbilical cord blood (UCB and mobilized peripheral blood (PBCD34(+ being the most accessible HSC sources as collection procedures are less invasive. In this study we compared the capacity of frozen or freshly isolated UCB hematopoietic stem cells (CBCD34(+ and frozen PBCD34(+ to generate NK cells in vitro. By modifying a previously published protocol, we showed that frozen CBCD34(+ cultures generated higher NK cell numbers without loss of function compared to fresh CBCD34(+ cultures. NK cells generated from CBCD34(+ and PBCD34(+ expressed low levels of killer-cell immunoglobulin-like receptors but high levels of activating receptors and of the myeloid marker CD33. However, blocking studies showed that CD33 expression did not impact on the functions of the generated cells. CBCD34(+-NK cells exhibited increased capacity to secrete IFN-γ and kill K562 in vitro and in vivo as compared to PBCD34(+-NK cells. Moreover, K562 killing by the generated NK cells could be further enhanced by IL-12 stimulation. Our data indicate that the use of frozen CBCD34(+ for the production of NK cells in vitro results in higher cell numbers than PBCD34(+, without jeopardizing their functionality, rendering them suitable for NK cell immunotherapy. The results presented here provide an optimal strategy to generate NK cells in vitro for immunotherapy that exhibit enhanced effector function when compared to alternate sources of HSC.

  2. Fixation and localisation of manganese in some soft water organisms: I - Distribution of 54Mn in an ecologic system in fresh water; II - study by biochemical fractionation of manganese contained by isolated plant cells

    International Nuclear Information System (INIS)

    Magnaval, Robert; Lachet, Bernard; Gagnaire, Janine; Fourcy, Andre; Neuburger, Michel; Fer, Andre

    1968-12-01

    After having recalled that Manganese 54, an irradiation product of 54 Fe, has a sufficiently long radioactive period to be detected in atmospheric fallouts of nuclear explosions, but also in radioactive wastes produced by research centres, and is generally produced by corrosion of any enclosure submitted to neutron irradiation, the authors report the experimental study of a radioactive pollution of fresh water when this radio-element is a component of this pollution. Different containers are considered. They contain either water and sediments, or water and organic compounds and aquatic plants, or water and sediments and aquatic plants. A solution of 54 MnCl 2 of radioactive pollution is introduced in these containers and the authors study the decrease of radioactivity in water with respect to the considered ecosystem, the 54 Mn concentration in aquatic living species, and the distribution of 54 Mn after 43 days. In a second part, they report the study of the distribution of cellular manganese in isolated biochemical fractions by using the Schmidt and Thannhauser method. Due to the low content, the detection of this mineral nutrient requires a highly sensitive method: dosing of natural manganese by radio-activation

  3. Nitrogen uptake kinetics of freshly isolated zooxanthellae

    Digital Repository Service at National Institute of Oceanography (India)

    Wafar, M.V.M.; Wafar, S.; Rajkumar, R.

    that for nitrate [2.8 nmol. ( mu chl-a)./1h/1] and urea [0.37 nmol. ( mu chl-a)./1h/1]. Half-saturation constants for uptake of the three nitrogen compounds were in the range of 10-15 mu mol.l/1. Generally, uptake of any one nitrogen substrate appears to be inhibit...

  4. Freshly isolated hepatocyte transplantation in acetaminophen-induced hepatotoxicity model in rats Transplante de hepatócitos recém-isolados em um modelo de hepatotoxicidade induzida por acetaminofeno em ratos

    Directory of Open Access Journals (Sweden)

    Daniela Rodrigues

    2012-12-01

    Full Text Available CONTEXT: Hepatocyte transplantation is an attractive therapeutic modality for liver disease as an alternative for orthotopic liver transplantation. OBJECTIVE: The aim of the current study was to investigate the feasibility of freshly isolated rat hepatocyte transplantation in acetaminophen-induced hepatotoxicity model. METHODS: Hepatocytes were isolated from male Wistar rats and transplanted 24 hours after acetaminophen administration in female recipients. Female rats received either 1x10(7 hepatocytes or phosphate buffered saline through the portal vein or into the spleen and were sacrificed after 48 hours. RESULTS: Alanine aminotransferase levels measured within the experiment did not differ between groups at any time point. Molecular analysis and histology showed presence of hepatocytes in liver of transplanted animals injected either through portal vein or spleen. CONCLUSION: These data demonstrate the feasibility and efficacy of hepatocyte transplantation in the liver or spleen in a mild acetaminophen-induced hepatotoxicity model.CONTEXTO: O transplante de hepatócitos é uma modalidade terapêutica atrativa para doenças hepáticas como alternativa ao transplante hepático ortotópico. OBJETIVO: Investigar a factibilidade do uso de hepatócitos frescos isolados de ratos em um modelo de hepatotoxicidade induzida por paracetamol. MÉTODOS: Hepatócitos foram isolados de ratos Wistar machos e transplantados 24 horas após a administração de paracetamol em receptores fêmeas. As ratas receberam 1x10(7 hepatócitos ou tampão salina fosfato pela veia porta ou no baço e foram sacrificadas após 48 horas. RESULTADOS: Os níveis de alanina aminotransferase medidos durante o experimento não diferiram entre os grupos em nenhum momento. Análises moleculares e histológicas demonstraram a presença de hepatócitos no fígado dos animais transplantados pelo baço ou pela veia porta. CONCLUSÃO: Os dados indicam a factibilidade e eficácia do

  5. Microbiological Quality of Fresh Nopal Juice.

    Science.gov (United States)

    Hernández-Anguiano, Ana María; Landa-Salgado, Patricia; Eslava-Campos, Carlos Alberto; Vargas-Hernández, Mateo; Patel, Jitendra

    2016-12-10

    The consumption of fresh nopal cactus juice is widely popular among health-conscious consumers in Mexico. The juice is prepared from fresh cladodes that have only been rinsed with tap water and are not subjected to a pasteurization or terminal bacterial reduction process. The aim of this study was to evaluate the microbial quality of commercially available fresh juices ( n = 162) made with nopal in Texcoco, State of Mexico, during the summer and spring season. Standard microbiological methods, the PCR technique and the serological method were used for isolation and identification of bacteria. All samples contained total coliforms and 91% were positive for Escherichia coli . Although total coliforms and E. coli were detected throughout the study, their populations were significantly lower ( p nopal juices is unacceptable due to its health significance. The information generated in this study is relevant for human health risk assessment associated with the consumption of unpasteurized nopal juices and potential interventions to minimize pathogen contamination.

  6. Fresh Water Technology

    International Nuclear Information System (INIS)

    Ko, Yang Mun; Kim, O Sik; Kim, Jin Nam; Kim, Cheol Su

    1985-02-01

    This book tells US summary of fresh water with evaporation system like basic principle and outline, multistage flash evaporation, multiple-effect evaporation, other evaporation, evaporation plant by development merger, design of evaporation plant, reverse osmosis on summary, type and production of membrane, reverse osmosis device, reverse osmosis process, electrodialysis with outline of electrodialysis and polarization and energy, freezing preservation and corrosion and scale.

  7. Intelligent Fish Freshness Assessment

    Directory of Open Access Journals (Sweden)

    Hamid Gholam Hosseini

    2008-01-01

    Full Text Available Fish species identification and automated fish freshness assessment play important roles in fishery industry applications. This paper describes a method based on support vector machines (SVMs to improve the performance of fish identification systems. The result is used for the assessment of fish freshness using artificial neural network (ANN. Identification of the fish species involves processing of the images of fish. The most efficient features were extracted and combined with the down-sampled version of the images to create a 1D input vector. Max-Win algorithm applied to the SVM-based classifiers has enhanced the reliability of sorting to 96.46%. The realisation of Cyranose 320 Electronic nose (E-nose, in order to evaluate the fish freshness in real-time, is experimented. Intelligent processing of the sensor patterns involves the use of a dedicated ANN for each species under study. The best estimation of freshness was provided by the most sensitive sensors. Data was collected from four selected species of fishes over a period of ten days. It was concluded that the performance can be increased using individual trained ANN for each specie. The proposed system has been successful in identifying the number of days after catching the fish with an accuracy of up to 91%.

  8. Irradiation of fresh fish

    International Nuclear Information System (INIS)

    Yueh-jen, Y.; Jin-lai, Z.; Shao-chun, L.

    1983-01-01

    Occasionally, in China, marine products can not be provided for the markets in good quality, for during the time when they are being transported from the sea port to inland towns or even at the time when they are unloaded from the ship, they are beginning to spoil. Obviously, it is very important that appropriate measures should be taken to prevent them from decay. Our study has proved that the shelf life of fresh Flatfish (Cynoglossue robustus) and Silvery pomfret (stromateoides argenteus), which, packed in sealed containers, are irradiated by 1.5 kGy, 2.2 kGy and 3.0 kGy, can be stored for about 13 to 26 days at 3 deg to 5 deg C. (author)

  9. Comparison of susceptibility to antibiotics of Staphylococcus aureus isolated from fresh cheeses and clinical samples from the Area Metropolitana and evaluation of the presence of Listeria monocytogenes in the same foodstuff

    International Nuclear Information System (INIS)

    Piedra Ramirez, Hiryna

    2014-01-01

    60 samples of fresh cheeses were analyzed from the central markets of Alajuela, Heredia, Cartago and San Jose. Staphylococcus aureus, total coliform and thermotolerant counts were performed, as well as Escherichia coli by the NMP technique. The presence of Listeria monocytogenes was evaluated in 25 g of cheese. The confirmatory identification and sensitivity profile of S. aureus strains were obtained using the Vitek system. L. monocytogenes were identified by means of biochemical tests. The statistical package Statistical Package for the Social Sciences (SPSS) and Excell were used to analyze the data. The presence of fecal contamination was identified as well as S. aureus in fresh cheeses from the four provinces studied. The comparative analysis of S. aureus antibiotic susceptibility profiles from clinical specimens and cheeses, for the majority of antibiotics under study was able to determine that the sensitivity patterns shown have been different. The clinical strains of origin could be observed with a pattern of multiresistance more marked than the strains of alimentary origin. The presence of L. monocytogenes was identified in an important percentage of cheese samples. (author) [es

  10. Microbiological Quality of Fresh Nopal Juice

    Directory of Open Access Journals (Sweden)

    Ana María Hernández-Anguiano

    2016-12-01

    Full Text Available The consumption of fresh nopal cactus juice is widely popular among health-conscious consumers in Mexico. The juice is prepared from fresh cladodes that have only been rinsed with tap water and are not subjected to a pasteurization or terminal bacterial reduction process. The aim of this study was to evaluate the microbial quality of commercially available fresh juices (n = 162 made with nopal in Texcoco, State of Mexico, during the summer and spring season. Standard microbiological methods, the PCR technique and the serological method were used for isolation and identification of bacteria. All samples contained total coliforms and 91% were positive for Escherichia coli. Although total coliforms and E. coli were detected throughout the study, their populations were significantly lower (p < 0.05 in winter and spring, respectively. Citrobacter youngae was found in 20% of the samples, an unidentified species of Citrobacter in 10%, C. freundii and Proteus mirabilis in 3%, and Salmonella Javiana in 1%. The presence of these microorganisms, especially Salmonella, in the nopal juices is unacceptable due to its health significance. The information generated in this study is relevant for human health risk assessment associated with the consumption of unpasteurized nopal juices and potential interventions to minimize pathogen contamination.

  11. Effects of environmental parameters on the dual-species biofilms formed by Escherichia coli O157:H7 and Ralstonia insidiosa, a strong biofilm producer isolated from a fresh-cut processing plant

    Science.gov (United States)

    Biofilm forming bacteria resident to food processing facilities are a food safety concern due to the potential of biofilms to harbor foodborne bacterial pathogens. When cultured together, Ralstonia insidiosa, a strong biofilm former frequently isolated from produce processing environments, has been ...

  12. Direct Comparison of the Histidine-rich Protein-2 Enzyme-linked Immunosorbent Assay (HRP-2 ELISA) and Malaria SYBR Green I Fluorescence (MSF) Drug Sensitivity Tests in Plasmodium falciparum Reference Clones and Fresh ex vivo Field Isolates from Cambodia

    Science.gov (United States)

    2013-07-12

    assessment of antimalarial activity in vitro by a semiautomated microdilution technique. Antimicrob Agents Chemother 1979, 16:710–718. 3. Noedl H, Attlmayr B...40:685–691. 32. Hawley SR, Bray PG, Mungthin M, Atkinson JD, O’Neill PM, Ward SA: Relationship between antimalarial drug activity , accumulation, and...success rate when testing DHA, AS, MQ, QN, CQ, and PPQ activities . A “successful” IC50 assay result for each P. falciparum clinical isolate was defined as

  13. Steroidal saponins from fresh stems of Dracaena angustifolia

    Science.gov (United States)

    Six new steroidal saponins (1-6), angudracanosides A-F, were isolated from fresh stems of Dracaena angustifolia, together with eight known compounds. The structures of compounds 1-6 were determined by detailed spectroscopic analyses and chemical methods. Antifungal testing of all compounds showed th...

  14. Hiding in Fresh Fruits and Vegetables: Opportunistic Pathogens May Cross Geographical Barriers

    OpenAIRE

    Al-Kharousi, Zahra S.; Guizani, Nejib; Al-Sadi, Abdullah M.; Al-Bulushi, Ismail M.; Shaharoona, Baby

    2016-01-01

    Different microbial groups of the microbiome of fresh produce can have diverse effects on human health. This study was aimed at identifying some microbial communities of fresh produce by analyzing 105 samples of imported fresh fruits and vegetables originated from different countries in the world including local samples (Oman) for aerobic plate count and the counts of Enterobacteriaceae, Enterococcus, and Staphylococcus aureus. The isolated bacteria were identified by molecular (PCR) and bioc...

  15. Molecular epidemiology of malaria in Cameroon. XV. Experimental studies on serum substitutes and supplements and alternative culture media for in vitro drug sensitivity assays using fresh isolates of Plasmodium falciparum.

    Science.gov (United States)

    Basco, Leonardo K

    2003-08-01

    In vitro drug sensitivity assay is an important tool for various on-going studies aiming to establish the correlation between candidate molecular markers for drug resistance and drug response in laboratory-adapted strains and field isolates of Plasmodium falciparum. A widespread use of this technique in the field would require a suitable substitute that can replace human serum. In this study, several alternative sources of serum substitutes and supplements were evaluated for their capacity to sustain parasite growth for a single life cycle and their compatibility with in vitro assays for clinical isolates that have not been adapted to in vitro culture. Albumax, a commercial preparation of lipid-enriched bovine albumin, did not support parasite growth as much as human serum and fetal calf serum in several isolates. Other serum supplements (AmnioMax and Ultroser) supported parasite growth relatively well. The 50% inhibitory concentrations (IC50s) of chloroquine and antifolates determined with 0.05% Albumax were generally two or three times higher than with human serum. With 10% fetal calf serum, IC50s for chloroquine and antifolates were approximately two times higher and three times lower than with human serum, respectively. The use of AmnioMax and OptiMAb resulted in a greater than two-fold increase in IC50s and several uninterpretable assays. Despite possible batch-to-batch differences, fetal calf serum may be a suitable substitute for in vitro drug assays while awaiting the results of further studies on other serum substitutes and supplements.

  16. ANTIBACTERIAL EFFECTS OF FRESH AND PRESERVED ROYAL JELLY

    Directory of Open Access Journals (Sweden)

    Zinka Maksimović

    2013-10-01

    Full Text Available Antibacterial effects of the fresh royal jelly, royal jelly stored at 4 °C and -40 °C for a period of 12 months against reference and isolated bacterial strains from the different clinical samples, were tested and compared by the diffusion test. Royal jelly shows antibacterial effects against both tested gram-positive and gram-negative bacteria. Fresh royal jelly has the most effective antibacterial activity. Storage temperature at -40 oC slightly affects antibacterial activity of royal jelly, while storage temperature at 4 oC decreases its antibacterial activity.Key words: royal jelly, antibacterial effects, storage temperature, storage duration

  17. 21 CFR 101.95 - “Fresh,” “freshly frozen,” “fresh frozen,” “frozen fresh.”

    Science.gov (United States)

    2010-04-01

    ... freezing will not preclude use of the term “fresh frozen” to describe the food. “Quickly frozen” means... 21 Food and Drugs 2 2010-04-01 2010-04-01 false âFresh,â âfreshly frozen,â âfresh frozen,â âfrozen fresh.â 101.95 Section 101.95 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND...

  18. Fresh vegetables from the desert

    NARCIS (Netherlands)

    Campen, J.B.

    2016-01-01

    In the Middle East, food security and food safety are high on the agenda since
    the food crisis of 2007-2008. Food is mainly imported from nearby countries.
    Especially during the summer the prices of fresh produce are high, because
    there is not much production in the region itself.

  19. Antimicrobial packaging for fresh-cut fruits

    Science.gov (United States)

    Fresh-cut fruits are minimally processed produce which are consumed directly at their fresh stage without any further kill step. Microbiological quality and safety are major challenges to fresh-cut fruits. Antimicrobial packaging is one of the innovative food packaging systems that is able to kill o...

  20. Hiding in Fresh Fruits and Vegetables: Opportunistic Pathogens May Cross Geographical Barriers

    Directory of Open Access Journals (Sweden)

    Zahra S. Al-Kharousi

    2016-01-01

    Full Text Available Different microbial groups of the microbiome of fresh produce can have diverse effects on human health. This study was aimed at identifying some microbial communities of fresh produce by analyzing 105 samples of imported fresh fruits and vegetables originated from different countries in the world including local samples (Oman for aerobic plate count and the counts of Enterobacteriaceae, Enterococcus, and Staphylococcus aureus. The isolated bacteria were identified by molecular (PCR and biochemical methods (VITEK 2. Enterobacteriaceae occurred in 60% of fruits and 91% of vegetables. Enterococcus was isolated from 20% of fruits and 42% of vegetables. E. coli and S. aureus were isolated from 22% and 7% of vegetables, respectively. Ninety-seven bacteria comprising 21 species were similarly identified by VITEK 2 and PCR to species level. E. coli, Klebsiella pneumoniae, Enterococcus casseliflavus, and Enterobacter cloacae were the most abundant species; many are known as opportunistic pathogens which may raise concern to improve the microbial quality of fresh produce. Phylogenetic trees showed no relationship between clustering of the isolates based on the 16S rRNA gene and the original countries of fresh produce. Intercountry passage of opportunistic pathogens in fresh produce cannot be ruled out, which requires better management.

  1. Cultivation, Isolation and Characterization of Bacteriocin from Fresh ...

    African Journals Online (AJOL)

    ADOWIE PERE

    agar diffusion method on culture plates seeded with Staphylococcus and E.coli. Bacteriocinogenic ... Bacilliocins (FCM6 and FMS1) and Micrococin FMS2 are thermal and pressure stable. BLPs that are ... medium that support broad spectrum of spoilage and benefious ..... inactivation of microorganisms in milk. Innovative ...

  2. Short communication Isolation of total ribonucleic acid from fresh ...

    African Journals Online (AJOL)

    Leyland

    2016-12-19

    Dec 19, 2016 ... South African Journal of Animal Science 2017, 47 (No. 1) ... plasma membrane integrity (PMI), normal apical ridges (NAR) .... contributed to the analysis of the RNA samples and CSP assisted in the revision of the manuscript.

  3. Prevalência, quantificação e resistência a antimicrobianos de sorovares de Salmonella isolados de lingüiça frescal suína Prevalence, quantification, and antimicrobial drug resistance of Salmonella serovars isolated from fresh pork sausage

    Directory of Open Access Journals (Sweden)

    Denis Augusto Spricigo

    2008-12-01

    Full Text Available A Salmonella sp. é uma das principais causas mundiais de toxinfecção alimentar. Nos últimos anos, as preocupações têm se voltado para a carne e produtos suínos tanto no aspecto de saúde pública como na sua comercialização/exportação. O presente estudo tem como objetivos: 1 verificar a prevalência de sorovares de Salmonella sp. em lingüiças tipo frescal de matéria-prima suína comercializadas em Lages (SC, bem como o seu nível de contaminação; e 2 verificar o perfil de resistência aos antimicrobianos destes isolados. Para tanto, foram coletadas 200 amostras de nove marcas, em diferentes estabelecimentos comerciais. Foram isoladas Salmonella sp. em 27% (54, sendo o sorovar Derby o mais encontrado. Apenas uma amostra apresentou uma concentração de microorganismos maior que 1,100 NMP.g-1, valor normalmente tido como necessário para causar infecção por Salmonella do grupo não-tifóide. Posteriormente, os 60 isolados foram submetidos ao teste de susceptibilidade in vitro, frente a 14 antimicrobianos. Entre esses isolados, 56,67% apresentaram resistência a pelo menos um dos antimicrobianos testados e o perfil de multirresistência foi encontrado em 20%. A prevalência elevada de produtos positivos para Salmonella sp. pode representar um risco ao consumidor, principalmente considerando-se o alto número de isolados resistentes encontrado neste estudo.Salmonella sp. is one of the main causes of food poisoning. In the last years, the main focus has been on beef and swine products because of both public health concerns and commercialization/exportation. This study was conducted in order to: 1 verify the prevalence of Salmonella serovars in fresh pork sausages commercialized in Lages, state of Santa Catarina and analyze its level of contamination; and 2 determine the profile of antimicrobial resistance of Salmonella sp. isolates. For this purpose, 200 samples of nine brands were collected from different commercial stores

  4. Systematic Study of the Content of Phytochemicals in Fresh and Fresh-Cut Vegetables

    Directory of Open Access Journals (Sweden)

    María Isabel Alarcón-Flores

    2015-05-01

    Full Text Available Vegetables and fruits have beneficial properties for human health, because of the presence of phytochemicals, but their concentration can fluctuate throughout the year. A systematic study of the phytochemical content in tomato, eggplant, carrot, broccoli and grape (fresh and fresh-cut has been performed at different seasons, using liquid chromatography coupled to triple quadrupole mass spectrometry. It was observed that phenolic acids (the predominant group in carrot, eggplant and tomato were found at higher concentrations in fresh carrot than in fresh-cut carrot. However, in the case of eggplant, they were detected at a higher content in fresh-cut than in fresh samples. Regarding tomato, the differences in the content of phenolic acids between fresh and fresh-cut were lower than in other matrices, except in winter sampling, where this family was detected at the highest concentration in fresh tomato. In grape, the flavonols content (predominant group was higher in fresh grape than in fresh-cut during all samplings. The content of glucosinolates was lower in fresh-cut broccoli than in fresh samples in winter and spring sampling, although this trend changes in summer and autumn. In summary, phytochemical concentration did show significant differences during one-year monitoring, and the families of phytochemicals presented different behaviors depending on the matrix studied.

  5. Characterization and Application of Autochthonous Starter Cultures for Fresh Cheese Production

    Directory of Open Access Journals (Sweden)

    Andreja Leboš Pavunc

    2012-01-01

    Full Text Available The use of commercial starter cultures in fresh cheese production from pasteurized milk results in the loss of typical characteristics of artisan fresh cheese due to the replacement of complex native microbiota with a defined starter culture. Hence, the aim of this research is to isolate and characterize dominant lactic acid bacteria (LAB in artisan fresh cheese and to evaluate their capacity as autochthonous starter cultures for fresh cheese production. Fifteen most prevalent Gram-positive, catalase-negative and asporogenous bacterial strains were selected for a more detailed characterization. Eleven lactic acid bacterial strains were determined to be homofermentative cocci and four heterofermentative lactobacilli. Further phenotypic and genotypic analyses revealed that those were two different LAB strains with high acidifying and proteolytic activity, identified as Lactobacillus fermentum A8 and Enterococcus faecium A7. These two autochthonous strains, alone or in combination with commercial starter, were used to produce different types of fresh cheese, which were evaluated by a panel. Conventional culturing, isolation, identification and PCR-denaturing gradient gel electrophoresis (PCR-DGGE procedures, applied to the total fresh cheese DNA extracts, were employed to define and monitor the viability of the introduced LAB strains and their effect on the final product characteristics. Production of fresh cheese using a combination of commercial starter culture and selected autochthonous strains resulted in improved sensorial properties, which were more similar to the ones of spontaneously fermented fresh cheese than to those of cheese produced with only starter culture or selected strains. After 10 days of storage, that cheese retained the best sensorial properties in comparison with all other types of cheese. The presence of inoculated autochthonous and starter cultures and their identification was demonstrated by DGGE analysis. The obtained

  6. Development of a selective agar plate for the detection of Campylobacter spp. in fresh produce.

    Science.gov (United States)

    Yoo, Jin-Hee; Choi, Na-Young; Bae, Young-Min; Lee, Jung-Su; Lee, Sun-Young

    2014-10-17

    This study was conducted to develop a selective medium for the detection of Campylobacter spp. in fresh produce. Campylobacter spp. (n=4), non-Campylobacter (showing positive results on Campylobacter selective agar) strains (n=49) isolated from fresh produce, indicator bacteria (n=13), and spoilage bacteria isolated from fresh produce (n=15) were plated on four Campylobacter selective media. Bolton agar and modified charcoal cefoperazone deoxycholate agar (mCCDA) exhibited higher sensitivity for Campylobacter spp. than did Preston agar and Hunt agar, although certain non-Campylobacter strains isolated from fresh produce by using a selective agar isolation method, were still able to grow on Bolton agar and mCCDA. To inhibit the growth of non-Campylobacter strains, Bolton agar and mCCDA were supplemented with 5 antibiotics (rifampicin, polymyxin B, sodium metabisulfite, sodium pyruvate, ferrous sulfate) and the growth of Campylobacter spp. (n=7) and non-Campylobacter strains (n=44) was evaluated. Although Bolton agar supplemented with rifampicin (BR agar) exhibited a higher selectivity for Campylobacter spp. than did mCCDA supplemented with antibiotics, certain non-Campylobacter strains were still able to grow on BR agar (18.8%). When BR agar with various concentrations of sulfamethoxazole-trimethoprim were tested with Campylobacter spp. (n=8) and non-Campylobacter (n=7), sulfamethoxazole-trimethoprim was inhibitory against 3 of 7 non-Campylobacter strains. Finally, we validated the use of BR agar containing 50mg/L sulfamethoxazole (BRS agar) or 0.5mg/L ciprofloxacin (BRCS agar) and other selective agars for the detection of Campylobacter spp. in chicken and fresh produce. All chicken samples were positive for Campylobacter spp. when tested on mCCDA, BR agar, and BRS agar. In fresh produce samples, BRS agar exhibited the highest selectivity for Campylobacter spp., demonstrating its suitability for the detection of Campylobacter spp. in fresh produce. Copyright

  7. Nuclear waste - a fresh perspective

    International Nuclear Information System (INIS)

    Tammemagi, H.Y.

    1996-01-01

    Rather than looking at the nuclear waste problem in isolation, it should be viewed in the broader context of how society disposes of all of its wastes. A comparison of radioactive and non-radioactive wastes shows, contrary to popular perception, that the properties of these two waste types are actually very similar. However, the methods of regulation and management of the two waste types are very different. It is time that these differences were reconciled - both the nuclear and the non-nuclear waste industries have a lot to gain. There are three main categories of (non-nuclear) waste: municipal wastes, hazardous wastes, and industrial wastes. Rather than treating each of these waste types in separate, isolated compartments, there should be an integration of the principles and regulations involved in their management. The non-nuclear waste industry has much to learn from the nuclear approach

  8. Fresh-keeping of mushroom by irradiation

    International Nuclear Information System (INIS)

    Liu Chao; Xu Hongqing; Wang Hong; Cai Jian

    2003-01-01

    The effect of 60 Co γ irradiation on the preservation of Agaricus bisporus were studied. The results showed that after irradiation the mushroom had lower rates of membrane split, opening of pilei, browning, decomposition and lose of fresh weight. The fresh keeping period of mushroom irradiated with 1.2 kGy and stored at 4 degree C was prolonged to 30 days

  9. Fresh fruit: microstructure, texture and quality

    Science.gov (United States)

    Fresh-cut produce has a huge following in today’s supermarkets. The trend follows the need to decrease preparation time as well as the desire to follow the current health guidelines for consumption of more whole “heart-healthy” foods. Additionally, consumers are able to enjoy a variety of fresh prod...

  10. What determines fresh fish consumption in Croatia?

    Science.gov (United States)

    Tomić, Marina; Matulić, Daniel; Jelić, Margareta

    2016-11-01

    Although fresh fish is widely available, consumption still remains below the recommended intake levels among the majority of European consumers. The economic crisis affects consumer food behaviour, therefore fresh fish is perceived as healthy but expensive food product. The aim of this study was to determine the factors influencing fresh fish consumption using an expanded Theory of Planned Behaviour (Ajzen, 1991) as a theoretical framework. The survey was conducted on a heterogeneous sample of 1151 Croatian fresh fish consumers. The study investigated the relationship between attitudes, perceived behavioural control, subjective norm, moral obligation, involvement in health, availability, intention and consumption of fresh fish. Structural Equation Modeling by Partial Least Squares was used to analyse the collected data. The results indicated that attitudes are the strongest positive predictor of the intention to consume fresh fish. Other significant predictors of the intention to consume fresh fish were perceived behavioural control, subjective norm, health involvement and moral obligation. The intention to consume fresh fish showed a strong positive correlation with behaviour. This survey provides valuable information for food marketing professionals and for the food industry in general. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Consumers' store choice behavior for fresh food

    NARCIS (Netherlands)

    Meulenberg, M.T.G.; Trijp, van J.C.M.

    1991-01-01

    Consumers' preference for fresh food stores is analyzed. In particular the choice between supermarkets and specialized shops for purchasing fresh food is analyzed. Attention is given to the factors influencing this choice. For this purpose a number of research questions with respect to store choice

  12. A new megastigmane from fresh roots of Rehmannia glutinosa

    Directory of Open Access Journals (Sweden)

    Weisheng Feng

    2013-09-01

    Full Text Available A new megastigmane, rehmamegastigmane (1, together with eighteen known compounds lariciresinol (2, lariciresinol-4′-O-β-d-glucopyranoside (3, hierochin D (4, yemuoside YM1 (5, darendoside B (6, decaffeoylacteoside (7, jionoside B1 (8, catalpol (9, ajugol (10, 6-O-vanilloylajugol (11, 6-O-E-feruloylajugol (12, rehmapicroside (13, rehmapicrogenin (14, 3-methoxy-2,6,6-trimethylcyclohexane-1-enecarboxylic acid (15, vanillic acid (16, hydroferulic acid (17, threo-1-(4-hydroxy-3-methoxyphenyl-1,2,3-propanetriol (18, p-hydroxyphenylethyl alcohol (19 was isolated from the fresh roots of Rehmannia glutinosa. Compounds 2–6 and 16–18 were isolated from this plant for the first time.

  13. Citrus fruits freshness assessment using Raman spectroscopy.

    Science.gov (United States)

    Nekvapil, Fran; Brezestean, Ioana; Barchewitz, Daniel; Glamuzina, Branko; Chiş, Vasile; Cintă Pinzaru, Simona

    2018-03-01

    The freshness of citrus fruits commonly available in the market was non-destructively assessed by Raman spectroscopy. Intact clementine, mandarin and tangerine species were characterised concerning their carotenoids skin Raman signalling in a time course from the moment they were acquired as fresh stock, supplying the market, to the physical degradation, when they were no longer attractive to consumers. The freshness was found to strongly correlate to the peel Raman signal collected from the same area of the intact fruits in a time course of a maximum of 20days. We have shown that the intensity of the carotenoid Raman signal is indeed a good indicator of fruit freshness and introduced a Raman coefficient of freshness (C Fresh ), whose time course is linearly decreasing, with different slope for different citrus groups. Additionally, we demonstrated that the freshness assessment could be achieved using a portable Raman instrument. The results could have a strong impact for consumer satisfaction and the food industry. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Bacteriological quality of fresh and smoke-dried oysters sold in ...

    African Journals Online (AJOL)

    Bacterial isolates found in fresh oyster were Klebsiella sp. Escherichia sp., Proteus mirabilis, Citrobacter sp., Micrococcus sp., Bacillus sp., Staphylococccus sp. and Serratia sp. while E. coli, Streptococcus sp., Staphylococcus sp. and B. cereus predominated in dried oysters. The oyster meats did not meet the standards of ...

  15. Waste Reduction in Fresh Food Supply Chains

    DEFF Research Database (Denmark)

    Kaipia, Riikka; Loikkanen, Lauri; Dukovska-Popovska, Iskra

    2011-01-01

    The paper studies a well-known phenomenon, information sharing in supply chains, in a new context, fresh foods, with a specific goal, supporting sustainable performance in the supply chain. Fresh foods are important for retail stores, representing around half of retail sales, but form a challengi...... and heterogeneous group of products to manage. The value of the paper lies in its pointing out detailed solutions to how in real-life supply chains data can be used efficiently to improve the performance of the supply chain.......The paper studies a well-known phenomenon, information sharing in supply chains, in a new context, fresh foods, with a specific goal, supporting sustainable performance in the supply chain. Fresh foods are important for retail stores, representing around half of retail sales, but form a challenging...

  16. Modelling global fresh surface water temperature

    NARCIS (Netherlands)

    Beek, L.P.H. van; Eikelboom, T.; Vliet, M.T.H. van; Bierkens, M.F.P.

    2011-01-01

    Temperature directly determines a range of water physical properties including vapour pressure, surface tension, density and viscosity, and the solubility of oxygen and other gases. Indirectly water temperature acts as a strong control on fresh water biogeochemistry, influencing sediment

  17. Biochemical Basis of Fresh Ham Color Development

    OpenAIRE

    Stufft, Kristen Marie

    2015-01-01

    Commercial hams display variation in color uniformity across the cut surface, especially the semimembranosus (SM) muscle. This variation in fresh ham color, or two-toning, persists through further processing and contributes to production of a less desirable end product. In an attempt to understand the underlying source of this color variation, we evaluated the differences in muscle fiber-type composition and glycolytic metabolism in the SM muscle of fresh hams differing in color uniformity. F...

  18. Microbes Associated with Freshly Prepared Juices of Citrus and Carrots

    Directory of Open Access Journals (Sweden)

    Kamal Rai Aneja

    2014-01-01

    Full Text Available Fruit juices are popular drinks as they contain antioxidants, vitamins, and minerals that are essential for human being and play important role in the prevention of heart diseases, cancer, and diabetes. They contain essential nutrients which support the growth of acid tolerant bacteria, yeasts, and moulds. In the present study, we have conducted a microbiological examination of freshly prepared juices (sweet lime, orange, and carrot by serial dilution agar plate technique. A total of 30 juice samples were examined for their microbiological quality. Twenty-five microbial species including 9 bacterial isolates, 5 yeast isolates, and 11 mould isolates were isolated from juices. Yeasts and moulds were the main cause of spoilage of juices. Aspergillus flavus and Rhodotorula mucilaginosa were observed in the maximum number of juice samples. Among bacteria Bacillus cereus and Serratia were dominant. Escherichia coli and Staphylococcus aureus were detected in few samples. Candida sp., Curvularia, Colletotrichum, and Acetobacter were observed only in citrus juice samples. Alternaria, Aspergillus terreus, A. niger, Cladosporium, and Fusarium were also observed in tested juice samples. Some of the microorganisms detected in these juice samples can cause disease in human beings, so there is need for some guidelines that can improve the quality of fruit juices.

  19. Potentials for export of fresh raspberries from Serbia to EU fresh markets

    Directory of Open Access Journals (Sweden)

    Nikolić Ivan

    2012-12-01

    Full Text Available The aim of this paper is to present potentials of the most significant EU national markets for imports of fresh raspberries from Serbia. We carried out analysis of three markets with highest trade deficit of fresh raspberries expressed in quantities: Germany, Netherlands and Austria. The paper further analyses production and foreign trade trends in selected countries. According to results of this analysis, we identified monthly periods with highest potential for exports of fresh raspberries from Serbia to target markets. The paper also analyses wholesale prices of fresh raspberries and EU policy of direct support to raspberry producers.

  20. Desenvolvimento de massa alimentícia fresca funcional com a adição de isolado protéico de soja e polidextrose utilizando páprica como corante Development of functional fresh food adding soy protein isolate and polidextrose using paprika as coloring agent

    Directory of Open Access Journals (Sweden)

    Luz Maria Paucar-Menacho

    2008-12-01

    áprica foram de 3,5, 8,0 e 1,5%, respectivamente.The trend of the current consumer is to use foods that are easy to prepare and that besides having nutritional quality provide health benefits. In this work, fresh food with functional characteristics was developed incorporating Soy Protein Isolate (SPI and polidextrose using paprika as the coloring agent. The consumption of IPS could contribute to decrease cholesterol levels while polidextrose, acting as a fiber, could promote the reduction of risk of colon cancer. The optimization of the formulation was carried out using a 23 complete factorial design considering independent variables the following contents: i SPI; ii polidextrose; and iii paprika. The effects of these variables on the quality of the food were evaluated through physical-chemical characteristics (color and cooking test, instrumental texture, and sensory analysis. Regarding the physical parameters, instrumental color of the raw pasta was affected only by the addition of paprika. The parameters cooking time (2.5 minutes, increase in mass (1.40 to 1.64%, and loss of solids (3.80 to 5.56% were not influenced by the ingredients used. Elasticity (instrumental texture parameter was reduced with the increase of polidextrose. Regarding sensory analysis of the raw food, an increase in the addition of SPI and a reduction in the addition of polidextrose positively affected global acceptance and purchase intention. An increases in SPI and paprika increased color acceptance. Regarding sensory analysis of the cooked food, SPI had a positive effect on texture while polidextrose and paprika negatively affected this parameter. Nevertheless, the ingredients used did not influence purchase intention of the cooked food. The ideal percentages of polidextrose, soy protein isolate, and paprika were 3.5, 8.0, and 1.5%, respectively.

  1. Establishing the Global Fresh Water Sensor Web

    Science.gov (United States)

    Hildebrand, Peter H.

    2005-01-01

    This paper presents an approach to measuring the major components of the water cycle from space using the concept of a sensor-web of satellites that are linked to a data assimilation system. This topic is of increasing importance, due to the need for fresh water to support the growing human population, coupled with climate variability and change. The net effect is that water is an increasingly valuable commodity. The distribution of fresh water is highly uneven over the Earth, with both strong latitudinal distributions due to the atmospheric general circulation, and even larger variability due to landforms and the interaction of land with global weather systems. The annual global fresh water budget is largely a balance between evaporation, atmospheric transport, precipitation and runoff. Although the available volume of fresh water on land is small, the short residence time of water in these fresh water reservoirs causes the flux of fresh water - through evaporation, atmospheric transport, precipitation and runoff - to be large. With a total atmospheric water store of approx. 13 x 10(exp 12)cu m, and an annual flux of approx. 460 x 10(exp 12)cu m/y, the mean atmospheric residence time of water is approx. 10 days. River residence times are similar, biological are approx. 1 week, soil moisture is approx. 2 months, and lakes and aquifers are highly variable, extending from weeks to years. The hypothesized potential for redistribution and acceleration of the global hydrological cycle is therefore of concern. This hypothesized speed-up - thought to be associated with global warming - adds to the pressure placed upon water resources by the burgeoning human population, the variability of weather and climate, and concerns about anthropogenic impacts on global fresh water availability.

  2. Dynamic isolation technologies in negative pressure isolation wards

    CERN Document Server

    Xu, Zhonglin

    2017-01-01

    This book presents novel design principles and technologies for dynamic isolation based on experimental studies. These approaches have now become the local standard in Beijing and are currently being promoted for use nationwide. Further, the book provides details of measures and guidelines for the design process. Departing from the traditional understanding that isolation wards should be designed with high negative pressure, airtight doors and fresh air, it establishes the basis for designing biological clean rooms, including isolation wards, using a simple and convenient scientific approach. This book is intended for designers, engineers, researchers, hospital management staff and graduate students in heating ventilation air conditioning (HVAC), air cleaning technologies and related areas.

  3. Detección, aislamiento y caracterización de Escherichia coli productor de toxina Shiga a partir de carne molida fresca proveniente de carnicerías de Concepción, provincia de Tucumán Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC in fresh ground beef from butcher shops in Concepción, Tucumán Province

    Directory of Open Access Journals (Sweden)

    M. A. Jure

    2010-12-01

    Full Text Available Escherichia coli productor de toxina Shiga (STEC es un patógeno emergente transmitido por alimentos. Existen numerosos serotipos de STEC asociados a enfermedad en humanos, entre los cuales prevalece el serotipo O157:H7. La carne molida es el principal vehículo de transmisión. En la ciudad de Concepción, provincia de Tucumán, entre setiembre y diciembre de 2004 se diagnosticaron dos casos de síndrome urémico hemolítico (SUH. El objetivo de este trabajo fue detectar, aislar y caracterizar STEC O157 y no-O157 a partir de muestras de carne molida fresca obtenidas en las bocas de expendio. Entre los meses de setiembre y diciembre de 2004 se recolectaron 53 muestras de carne molida fresca en carnicerías de la ciudad de Concepción. Para la detección, el aislamiento y la caracterización de STEC O157:H7 se utilizó la metodología USDA-FSIS 2002. Para la detección de E. coli no-O157 se utilizaron dos técnicas de PCR; para el aislamiento y la caracterización se utilizó una metodología previamente validada en una etapa intralaboratorio. Siete muestras fueron positivas para el gen stx2, de las cuales 4 también fueron positivas para el gen rfbO157. Sin embargo, solo se aisló una cepa de E. coli O157:H7 biotipo C, portadora de los genes eae, stx2 y ehxA. El presente trabajo refleja la importancia de implementar técnicas que permitan detectar este grupo de patógenos emergentes a partir de productos cárnicos.Shiga toxin-producing Escherichia coli is an emerging foodborne pathogen. There are many STEC serotypes associated with human diseases, being the O157:H7 serotype the most prevalent. Ground beef is the main transmission vehicle. In Concepción city, Tucumán Province, between September and December 2004, two hemolytic uremic syndrome (HUS cases were diagnosed. The main objective of this work was to detect, isolate and characterize STEC O157 and non-O157 strains in fresh ground beef. Between September and December 2004, 53 fresh ground

  4. Preservation technologies for fresh meat - a review.

    Science.gov (United States)

    Zhou, G H; Xu, X L; Liu, Y

    2010-09-01

    Fresh meat is a highly perishable product due to its biological composition. Many interrelated factors influence the shelf life and freshness of meat such as holding temperature, atmospheric oxygen (O(2)), endogenous enzymes, moisture, light and most importantly, micro-organisms. With the increased demand for high quality, convenience, safety, fresh appearance and an extended shelf life in fresh meat products, alternative non-thermal preservation technologies such as high hydrostatic pressure, superchilling, natural biopreservatives and active packaging have been proposed and investigated. Whilst some of these technologies are efficient at inactivating the micro-organisms most commonly related to food-borne diseases, they are not effective against spores. To increase their efficacy against vegetative cells, a combination of several preservation technologies under the so-called hurdle concept has also been investigated. The objective of this review is to describe current methods and developing technologies for preserving fresh meat. The benefits of some new technologies and their industrial limitations is presented and discussed.

  5. Consumer's Fresh Produce Food Safety Practices: Outcomes of a Fresh Produce Safety Education Program

    Science.gov (United States)

    Scott, Amanda R.; Pope, Paul E.; Thompson, Britta M.

    2009-01-01

    The Centers for Disease Control and Prevention estimate that there are 76 million cases of foodborne disease annually. Foodborne disease is usually associated with beef, poultry, and seafood. However, there is an increasing number of foodborne disease cases related to fresh produce. Consumers may not associate fresh produce with foodborne disease…

  6. Fresh water generators onboard a floating platform

    International Nuclear Information System (INIS)

    Tewari, P.K.; Verma, R.K.; Misra, B.M.; Sadhulkan, H.K.

    1997-01-01

    A dependable supply of fresh water is essential for any ocean going vessel. The operating and maintenance personnel on offshore platforms and marine structures also require a constant and regular supply of fresh water to meet their essential daily needs. A seawater thermal desalination unit onboard delivers good quality fresh water from seawater. The desalination units developed by Bhabha Atomic Research Centre (BARC) suitable for ocean going vessels and offshore platforms have been discussed. Design considerations of such units with reference to floating platforms and corrosive environments have been presented. The feasibility of coupling a low temperature vacuum evaporation (LTVE) desalination plant suitable for an onboard floating platform to a PHWR nuclear power plant has also been discussed. (author). 1 ref., 3 figs, 2 tabs

  7. Inheritance of fresh-cut fruit quality attributes in Capsicum

    Science.gov (United States)

    The fresh-cut fruit and vegetable industry has expanded rapidly during the past decade, due to freshness, convenience and the high nutrition that fresh-cut produce offers to consumers. The current report evaluates the inheritance of postharvest attributes that contribute to pepper fresh-cut product...

  8. Hot water, fresh beer, and salt

    International Nuclear Information System (INIS)

    Crawford, F.S.

    1990-01-01

    In the ''hot chocolate effect'' the best musical scales (those with the finest tone quality, largest range, and best tempo) are obtained by adding salt to a glass of hot water supersaturated with air. Good scales can also be obtained by adding salt to a glass of freshly opened beer (supersaturated with CO 2 ) provided you first (a) get rid of much of the excess CO 2 so as to produce smaller, hence slower, rising bubbles, and (b) get rid of the head of foam, which damps the standing wave and ruins the tone quality. Finally the old question, ''Do ionizing particles produce bubbles in fresh beer?'' is answered experimentally

  9. Ionising energy treatment of fresh fruit

    International Nuclear Information System (INIS)

    Rigney, C.J.

    1985-01-01

    The main purposes of the ionising energy treatment of fresh fruit are: the extension of shelf life of the commodity due to a direct physiological effect on the particular product; the extension of shelf life of the commodity due to a reduction in the development of moulds and rots which would normally render the product worthless; and the killing of insect pests of quarantine significance, to allow for normal marketing of fresh fruit without the risk of introducing insect pests to previously pest-free areas

  10. Hot water, fresh beer, and salt

    Science.gov (United States)

    Crawford, Frank S.

    1990-11-01

    In the ``hot chocolate effect'' the best musical scales (those with the finest tone quality, largest range, and best tempo) are obtained by adding salt to a glass of hot water supersaturated with air. Good scales can also be obtained by adding salt to a glass of freshly opened beer (supersaturated with CO2) provided you first (a) get rid of much of the excess CO2 so as to produce smaller, hence slower, rising bubbles, and (b) get rid of the head of foam, which damps the standing wave and ruins the tone quality. Finally the old question, ``Do ionizing particles produce bubbles in fresh beer?'' is answered experimentally.

  11. Study on E. coli and Salmonella biofilms from fresh fruits and vegetables.

    Science.gov (United States)

    Amrutha, Balagopal; Sundar, Kothandapani; Shetty, Prathapkumar Halady

    2017-04-01

    Foodborne outbreaks associated with fresh fruits and vegetables are on the rise worldwide. Biofilm formation is one of the important traits of pathogens making them strongly attached to substrates as well as express virulence phenotypes. Present study investigates the biofilm forming ability of E. coli and Salmonella sp. isolated from fresh fruits and vegetables. A total of 53 strains, including 35 E. coli and 18 Salmonella sp. isolated from different fruit and vegetable samples were taken into account for the study. Initial screening for biofilm formation was done using Congo Red agar plate test. Results revealed that 22.8% E. coli and 22.2% Salmonella sp. were potential biofilm formers. However, the MTP (Micro-Titre Plate) assay suggested more isolates of both E. coli and Salmonella sp. were moderate to strong biofilm producers. Agar plate diffusion assay with Agrobacterium tumefaciens NTL-4 showed the production of quorum signaling molecules (AHLs) by three isolates of E. coli and one Salmonella sp. Two E. coli isolates showed a significant amount of EPS production indicating higher biofilm forming potential. The Presence of LUX R homologue gene ( sdi A) in two of the Salmonella isolates were confirmed by PCR which demonstrated their potential pathogenicity. Results of the work underline the biofilm forming and potentially virulent capacities of isolates from the surface of fruits and vegetables.

  12. Changes of Bacterial Diversity Depend on the Spoilage of Fresh Vegetables

    Directory of Open Access Journals (Sweden)

    Dong Hwan Lee

    2011-04-01

    Full Text Available Almost 10~30% of vegetables were discarded by the spoilage from farms to tables. After harvest, vegetables are often spoiled by a wide variety of microorganisms including many bacterial and fungal species. This investigation was conducted to extent the knowledge of relationship the spoilage of vegetables and the diversity of microbes. The total aerobic bacterial numbers in fresh lettuce, perilla leaf, and chicory were 2.6~2.7×106, 4.6×105, 1.2×106 CFU/g of fresh weight, respectively. The most common bacterial species were Pseudomonas spp., Alysiella spp., and Burkholderia spp., and other 18 more genera were involved in. After one week of incubation of those vegetables at 28℃, the microbial diversity had been changed. The total aerobic bacterial numbers increased to 1.1~4.6×108, 4.9×107, and 7.6×108 CFU/g of fresh weight for lettuce, perilla leaf, and chicory that is about 102 times increased bacterial numbers than that before spoilage. However, the diversity of microbes isolated had been simplified and fewer bacterial species had been isolated. The most bacterial population (~48% was taken up by Pseudomonas spp., and followed by Arthrobacter spp. and Bacillus spp. The spoilage activity of individual bacterial isolates had been tested using axenic lettuce plants. Among tested isolates, Pseudomonas fluorescence and Pantoea agglomerans caused severe spoilage on lettuce.

  13. Process control system for fresh concrete preparation

    International Nuclear Information System (INIS)

    Bachvarov, N.; Pavlov, P.; Shukov, H.

    1983-01-01

    The paper discusses the principles, structure and organization of a modular microprocessor based control system, designed to be used in fresh concrete fabrication plants. The system is based on the measurement of the aggregate moisture by means of a neutron moisture meter. (author)

  14. Improving fresh groundwater supply - problems and solutions

    NARCIS (Netherlands)

    Oude Essink, Gualbert

    2001-01-01

    Many coastal regions in the world experience an intensive salt water intrusion in aquifers due to natural and anthropogenic causes. The salinisation of these groundwater systems can lead to a severe deterioration of the quality of existing fresh groundwater resources. In this paper, the

  15. Inhalational anaesthesia with low fresh gas flow

    Directory of Open Access Journals (Sweden)

    Christian Hönemann

    2013-01-01

    Full Text Available During the inhalation of anaesthesia use of low fresh gas flow (0.35-1 L/min has some important advantages. There are three areas of benefit: pulmonary - anaesthesia with low fresh gas flow improves the dynamics of inhaled anaesthesia gas, increases mucociliary clearance, maintains body temperature and reduces water loss. Economic - reduction of anaesthesia gas consumption resulting in significant savings of > 75% and Ecological - reduction in nitrous oxide consumption, which is an important ozone-depleting and heat-trapping greenhouse gas that is emitted. Nevertheless, anaesthesia with high fresh gas flows of 2-6 L/min is still performed, a technique in which rebreathing is practically negligible. This special article describes the clinical use of conventional plenum vaporizers, connected to the fresh gas supply to easily perform low (1 L/min, minimal (0.5 L/min or metabolic flow anaesthesia (0.35 L/min with conventional Primus Draeger® anaesthesia machines in routine clinical practice.

  16. Simple air collectors for preheating fresh air

    NARCIS (Netherlands)

    Hensen, J.L.M.; Wit, de M.H.; Ouden, den C.

    1984-01-01

    In dwellings with mechanical ventilation systems the fresh air can easily be preheated by means of simple solar air systems. These can be an integral part of the building facade or roof and the costs are expected to be low. By means of computer experiments a large number of systems were evaluated.

  17. The 'Fresh-Bunch' technique in FELs

    International Nuclear Information System (INIS)

    Ben-Zvi, I.; Yang, K.M.; Yu, L.H.

    1991-01-01

    The 'Fresh Bunch' technique is being proposed as a method of increasing the gain and power of FEL amplifiers in which the length of the optical radiation pulse is shorter than the length of the electron bunch. In multi-stage FEL, electron beam energy spread is increased by the FEL interaction in the early stages. In the 'Fresh Bunch' technique, the low energy spread of the electron beam is recovered by shifting the radiation pulse to an undisturbed part of the electron bunch, thus improving the gain and trapping fraction in later stages. A test case for the application of the Fresh Bunch method is demonstrated by numerical simulation. In this particular example we examine a subharmonically seeded VUV Free-Electron Laser. We begin with the generation of harmonic radiation, which takes place over one part of the electron bunch. Then the radiation is shifted by means of a strong dispersive section to a fresh part of the bunch for exponential amplification and tapered wiggler amplification. By starting over with a new ensemble of electrons, the energy spread introduced by the bunching in the fundamental is removed, leading to an increased gain. Furthermore, it is possible to use a much stronger seed in the fundamental without incurring the penalty of a large energy spread later on. We note that more than a single application of the 'Fresh Bunch' method may be done in a single FEL multiplier-amplifier. Thus x-ray wavelengths may be reached by successive multiplication in a chain of FEL amplifiers starting from a tunable seed laser. 5 refs., 2 figs., 2 tabs

  18. Biotechnological Approach To Preserve Fresh Pasta Quality.

    Science.gov (United States)

    Angiolillo, L; Conte, A; Del Nobile, M A

    2017-12-01

    Fresh pasta is highly susceptible to microbial contamination because of its high water activity and nutrient content. In this study, a new biopreservation system was examined that consists of an active sodium alginate solution containing Lactobacillus reuteri and glycerol, which was added during the production process of pasta. Our aim was to extend the fresh pasta shelf life by the in situ production of reuterin, thereby avoiding the use of thermal treatments that generally compromise food sensory characteristics. Two experimental studies were carried out with the product packaged under either ordinary or modified atmospheric conditions. Microbiological and sensory quality indices were monitored to determine the effectiveness of biopreservation on product quality during storage. The use of the active solution with L. reuteri and glycerol during the production process of pasta improved both microbial and sensory quality, particularly when combined with modified atmosphere.

  19. Vacuolar-type H+-ATPase and Na+, K+-ATPase expression in gills of Atlantic salmon (Salmo salar) during isolated and combined exposure to hyperoxia and hypercapnia in fresh water

    DEFF Research Database (Denmark)

    Seidelin, Michel; Brauner, Colin J; Jensen, Frank Bo

    2001-01-01

    Changes in branchial vacuolar-type H+-ATPase B-subunit mRNA and Na+, K+-ATPase alpha- and beta-subunit mRNA and ATP hydrolytic activity were examined in smolting Atlantic salmon exposed to hyperoxic and/or hypercapnic fresh water. Pre-smolts, smolts, and post-smolts were exposed for 1 to 4 days...... Na+, K+-ATPase activity was generally unaffected by the experimental treatments. We suggest that the reduced expression of branchial vacuolar-type H(+)-ATPase B-subunit mRNA observed during internal hypercapnic acidosis may lead to reduction of functional V-type H+-ATPase abundance as a compensatory...

  20. New ways of keeping fish fresh

    International Nuclear Information System (INIS)

    1970-01-01

    Take a fillet of cod and expose it to a controlled quantity of nuclear radiation. What happens? Its appearance is unchanged, but the fish - kept in a cool store - will still be edible and practically indistinguishable from fresh fish days, sometimes weeks, after ordinarily treated fish has had to be thrown away. Advantages seem from this addition to preservation methods are reported following experimental trials on cod, lobsters and shrimps. (author)

  1. Preservation of fresh noodles by irradiation

    International Nuclear Information System (INIS)

    Cai Jianming

    1998-01-01

    At the present paper, it is studied to preserve fresh noodles by irradiation. The noodles which were irradiated by 10 kGy of 60 Co-γ rays and stored at room temperature (18-24 deg. C). The appearance of the noodles was observed, the bacteria in the noodles were examined, and the acidity of the noodles was measured during 10 days after irradiation. The results showed that the number of bacteria and the extent of acidification of the noodles were in inverse proportion to radiation dose. When the absorbed dose was less than 8 kGy, the residual bacteria in the noodles could proliferate massively in several days and acidified the noodles. The pH of these noodles decreased from 6.2 to 5.0 or less and the noodles turned into sticky during storage. When the absorbed dose reached 8-10 kGy, most of bacteria were killed and the acidity of the noodles kept about pH 6.0. The appearance of the noodles looks fresh within 10 days after irradiation. They smelled as good as fresh ones

  2. Pasteurization and radiation of fresh milk

    International Nuclear Information System (INIS)

    Sjarief, Sri Hariani

    1976-01-01

    In this investigation, treatments with pasteurization and irradiation with gamma rays on fresh milk were used. Pasteurization was done at temperatures of 62 deg C and 70 deg C. The irradiation doses used were 100, 200, 300, 400, 500 and 600 krad. The results of the investigation showed that the number of bacteria decreased after either pasteurization or irradiation. The higher the dose of irradiation the lower the total bacterial counts in milk became, but the latter increased during storage at 10 deg C. The number of bacteria in pasteurized milk did not show any significant difference from milk irradiated with the dose of 300-600 krad after 15 days of storage in the refrigerator. No enzymatic activity was detected in pasteurized milk, but after storage the coli bacteria acivity tested to become positive in the MPN test. On the countrary irradiation of fresh milk could stimulate the enzymatic activity, although the protein content was constant. Finally, heating as a pretreatment is better to be used before pasteurizing or sterilizing milk with ionizing radiation in order to preserve fresh milk for a longer period without any changes in the nutritional quality and taste. (author)

  3. Preservation of fresh avocados by irradiation

    International Nuclear Information System (INIS)

    Karmelic, J.

    1985-01-01

    Avocado is the fifth most important fruit in Chile, which exports fruits to Europe. The production of avocado has increased from 14,000 metric tons in 1978 to a projected 70,000 metric tons in 1985. The main problem of shipping fresh Chilean avocado to Europe has been its short shelf life, being around 24 days with a maximum of 30 days. To market fresh Chilean avocados in Europe, adequate keeping quality must be assured for 40 or more days. A project on the preservation of fresh Chilean avocados supported by the International Atomic Energy Agency (IAEA) takes the approach of combining mild heat and low-dose gamma-radiation. Two varieties of avocados were studied (Hass and Fuerte) and two batches of each were treated: one at the beginning and the other at the end of the picking season. In each case a combination of heat treatment (46 0 C for 10 minutes) and irradiation was applied. Fruits were individually wrapped with flexible PVC and placed in cardboard boxes containing 20 each. Fruits treated with 25,50, and 100 Gy were compared with two controls: one with and another without individual wrapping

  4. Factors influencing Consumer Preference for Fresh Beef in Sokoto ...

    African Journals Online (AJOL)

    SH

    selected and interviewed to identify factors that affect preference for fresh beef and to determine the nature of the ... preference are appreciated by the food ... composition and household income .... habit and flavor, made them to prefer fresh.

  5. Diversity of Antimicrobial Resistance and Virulence Determinants in Pseudomonas aeruginosa Associated with Fresh Vegetables

    Directory of Open Access Journals (Sweden)

    Kashina Allydice-Francis

    2012-01-01

    Full Text Available With the increased focus on healthy eating and consuming raw vegetables, this study assessed the extent of contamination of fresh vegetables by Pseudomonas aeruginosa in Jamaica and examined the antibiotic susceptibility profiles and the presence of various virulence associated determinants of P. aeruginosa. Analyses indicated that vegetables from retail markets and supermarkets were widely contaminated by P. aeruginosa; produce from markets were more frequently contaminated, but the difference was not significant. Lettuce and carrots were the most frequently contaminated vegetables, while tomatoes were the least. Pigment production (Pyoverdine, pyocyanin, pyomelanin and pyorubin, fluorescein and alginate were common in these isolates. Imipenem, gentamicin and ciprofloxacin were the most inhibitory antimicrobial agents. However, isolates were resistant or showed reduced susceptibility to ampicillin, chloramphenicol, sulphamethoxazole/trimethoprim and aztreonam, and up to 35% of the isolates were resistant to four antimicrobial agents. As many as 30% of the isolates were positive for the fpv1 gene, and 13% had multiple genes. Sixty-four percent of the isolates harboured an exoenzyme gene (exoS, exoT, exoU or exoY, and multiple exo genes were common. We conclude that P. aeruginosa is a major contaminant of fresh vegetables, which might be a source of infection for susceptible persons within the community.

  6. Bundle Pricing Decisions for Fresh Products with Quality Deterioration

    OpenAIRE

    Fang, Yan; Jiang, Yiping; Han, Xingxing

    2018-01-01

    How to sell fresh products quickly to decrease the storage cost and to meet customer quality requirement is of vital importance in the food supply chain. Bundling fresh products is an efficient strategy to promote sales and reduce storage pressure of retailers. In this paper, we consider the bundle pricing decisions for homogeneous fresh products with quality deterioration. The value of fresh products with quality deterioration is approximated as an exponential function based on which custome...

  7. Pathogen detection, testing, and control in fresh broccoli sprouts

    Directory of Open Access Journals (Sweden)

    Fahey Jed W

    2006-04-01

    Full Text Available Abstract Background The recent increased interest in consuming green vegetable sprouts has been tempered by the fact that fresh sprouts can in some cases be vehicles for food-borne illnesses. They must be grown according to proper conditions of sanitation and handled as a food product rather than as an agricultural commodity. When sprouts are grown in accordance with the criteria proposed from within the sprout industry, developed by regulatory agencies, and adhered to by many sprouters, green sprouts can be produced with very low risk. Contamination may occur when these guidelines are not followed. Methods A one year program of microbial hold-and-release testing, conducted in concert with strict seed and facility cleaning procedures by 13 U.S. broccoli sprout growers was evaluated. Microbial contamination tests were performed on 6839 drums of sprouts, equivalent to about 5 million consumer packages of fresh green sprouts. Results Only 24 (0.75% of the 3191 sprout samples gave an initial positive test for Escherichia coli O157:H7 or Salmonella spp., and when re-tested, 3 drums again tested positive. Composite testing (e.g., pooling up to 7 drums for pathogen testing was equally sensitive to single drum testing. Conclusion By using a "test-and-re-test" protocol, growers were able to minimize crop destruction. By pooling drums for testing, they were also able to reduce testing costs which now represent a substantial portion of the costs associated with sprout growing. The test-and-hold scheme described herein allowed those few batches of contaminated sprouts to be found prior to packaging and shipping. These events were isolated, and only safe sprouts entered the food supply.

  8. 77 FR 26579 - Fresh Garlic From China; Determination

    Science.gov (United States)

    2012-05-04

    ... INTERNATIONAL TRADE COMMISSION [Investigation No. 731-TA-683 (Third Review)] Fresh Garlic From... Tariff Act of 1930 (19 U.S.C. 1675(c)), that revocation of the antidumping duty order on fresh garlic... contained in USITC Publication 4316 (April 2012), entitled Fresh Garlic from China: Investigation No. 731-TA...

  9. Microbial and preservative safety of fresh and processed fruit salads ...

    African Journals Online (AJOL)

    The producers and traders of fresh fruit and the processers should implement quality management practices and safety standards in farming, fresh fruit, processing and storage. This is to ensure safety, enhance consumption of fruits and fruit products for health of consumers and eliminate wastage. Key words: Fresh fruit, ...

  10. 78 FR 32184 - Importation of Fresh Apricots From Continental Spain

    Science.gov (United States)

    2013-05-29

    .... APHIS-2011-0132] RIN 0579-AD62 Importation of Fresh Apricots From Continental Spain AGENCY: Animal and... United States of fresh apricots from continental Spain. This action will allow interested persons... importation of fruits and vegetables to allow the importation of fresh apricots from continental Spain into...

  11. Allelopathic effect of aqueous extract of fresh leaf castor beans ...

    African Journals Online (AJOL)

    Rukevwe S. Abraka

    2016-12-07

    Dec 7, 2016 ... In this study, the allelopathic effect of aqueous extract from fresh leaves of castor .... triturated with electric blender and sieved for the extract preparation. .... (A), Leaf fresh mass; (B), root fresh mass; (C), stem diameter; (D), aerial part length. .... Naphthoquinones as allelochemical triggers of programmed cell.

  12. What is a Fresh Scent in Perfumery? Perceptual Freshness is Correlated with Substantivity

    Directory of Open Access Journals (Sweden)

    Manuel Zarzo

    2012-12-01

    Full Text Available Perfumes are manufactured by mixing odorous materials with different volatilities. The parameter that measures the lasting property of a material when applied on the skin is called substantivity or tenacity. It is well known by perfumers that citrus and green notes are perceived as fresh and they tend to evaporate quickly, while odors most dissimilar to ‘fresh’ (e.g., oriental, powdery, erogenic and animalic scents are tenacious. However, studies aimed at quantifying the relationship between fresh odor quality and substantivity have not received much attention. In this work, perceptual olfactory ratings on a fresh scale, estimated in a previous study, were compared with substantivity parameters and antierogenic ratings from the literature. It was found that the correlation between fresh odor character and odorant substantivity is quite strong (r = −0.85. ‘Fresh’ is sometimes interpreted in perfumery as ‘cool’ and the opposite of ‘warm’. This association suggests that odor freshness might be somehow related to temperature. Assuming that odor perception space was shaped throughout evolution in temperate climates, results reported here are consistent with the hypothesis that ‘fresh’ evokes scents typically encountered in the cool season, while ‘warm’ would be evoked by odors found in nature during summer. This hypothesis is rather simplistic but it may provide a new insight to better understand the perceptual space of scents.

  13. Fresh Slice Self-Seeding and Fresh Slice Harmonic Lasing at LCLS

    Energy Technology Data Exchange (ETDEWEB)

    Amann, J.W. [SLAC National Accelerator Lab., Menlo Park, CA (United States)

    2018-04-01

    We present results from the successful demonstration of fresh slice self-seeding at the Linac Coherent Light Source (LCLS).* The performance is compared with SASE and regular self-seeding at photon energy of 5.5 keV, resulting in a relative average brightness increase of a factor of 12 and a factor of 2 respectively. Following this proof-of-principle we discuss the forthcoming plans to use the same technique** for fresh slice harmonic lasing in an upcoming experiment. The demonstration of fresh slice harmonic lasing provides an attractive solution for future XFELs aiming to achieve high efficiency, high brightness X-ray pulses at high photon energies (>12 keV).***

  14. To transfer fresh or thawed embryos?

    DEFF Research Database (Denmark)

    Pinborg, Anja

    2012-01-01

    Worldwide freezing and thawing of embryos has been increasingly used since the first infant was born as a result of this technique in 1984. The use of frozen embryo replacement (FER) currently even exceeds the number of fresh cycles performed in some countries. This article discusses the pros...... and multiple pregnancies, thereby increasing the safety for mother and child. Finally the article describes the accumulating literature on perinatal and long-term child outcome after transfer of frozen/thawed embryos, including a discussion on the concerns regarding cryo techniques and their possible roles...

  15. Disposal of radioactive wastes into fresh water

    Energy Technology Data Exchange (ETDEWEB)

    1963-03-01

    The fate of radionuclides introduced into fresh water will be influenced not only by the volume of the water available for dilution, but also by a number of other physical, chemical and biological factors like replacement of the water mass, turbulence and location of the currents, chemical composition of effluent and receiving water, suspended materials, thermal characteristics and density differences, precipitation and sedimentation, ion-exchange and adsorption, incorporation by living organisms, and special features characteristics of the type of water body involved, i.e. whether stream, lake, estuary or sub-surface. 50 refs, 8 figs, 24 tabs.

  16. This is “Fresh Cream”

    OpenAIRE

    Troncy, Eric

    2012-01-01

    Fresh Cream, which is conceived as “an exhibition in a book”, and presented as playing “the role of a contemporary art Biennale”, is a sequel to Cream, which was also published by Phaidon in 1998. Based on the same principle, the book brings together 100 artists chosen by nobody in particular, but more exactly by 10 curators in general. The whole, per force, produces an impersonal result, and makes it possible, once again, to confirm the old adage that “a dromedary is a horse designed by a te...

  17. Quality of fresh-cut strawberry

    OpenAIRE

    Costa,Franciscleudo B; Duarte,Priscila S; Puschmann,Rolf; Finger,Fernando L

    2011-01-01

    The aim of this work was to study the physical, chemical, physiological and microbiological changes during the flow chart of fresh-cut strawberry. Strawberry cvs. Camarosa, Dover and Tudla, derived from experimental area of the Universidade Federal de Viçosa, were selected by color (red ¾) and absence of wound. Afterwards, the minimal processing was evaluated, as follows: fast cooling, water and ice, removal of the calyx followed by conservation at 5±0.5°C and 90-95% RH for 13 days, sanitatio...

  18. Methods to evaluate fish freshness in research and industry

    DEFF Research Database (Denmark)

    Olafsdottir, G.; Martinsdóttir, E.; Oehlenschläger, J.

    1997-01-01

    Current work in a European concerted action project 'Evaluation of Fish Freshness' (AIR3 CT94-2283) focuses on harmonizing research activities in the area of fish freshness evaluation in leading fish laboratories in Europe (see Box 1). The overall aim of the concerted action project is to validat...... measurements with respect to fish freshness evaluation. In this article, the different subgroups have summarized changes that occur in fish and methods to evaluate fish freshness as a first step towards the definition of criteria for fish freshness...

  19. Study of the microbial ecology of wild and aquacultured Tunisian fresh fish.

    Science.gov (United States)

    Boulares, Mouna; Mejri, Lobna; Hassouna, Mnasser

    2011-10-01

    Eighty samples of fresh fish were collected in Tunisia and analyzed for microbial load. Quality and hygienic safety of the meat and intestines of wild and aquacultured fresh fish were determined. The mesophilic aerobic plate count and populations of psychrotrophic lactic acid bacteria (LAB) and other psychrotrophic bacteria ranged from 5.67 to 7.29, 4.51 to 6, and 5.07 to 6.21 log CFU/g, respectively. For all microbiological determinations, bacterial counts were lower in meat than in the intestines of fresh fish. For all samples lower microbial populations were found in most of the wild fish than in the aquacultured fish. No isolates of the pathogenic genera Salmonella and Listeria were detected in any sample. Among the 160 strains of biopreservative psychrotrophic LAB and the 150 strains of spoilage psychrotrophic gram-negative bacteria identified by biochemical and molecular methods, Lactobacillus (six species) and Pseudomonas (six species) predominated. Lactococcus, Leuconostoc, Carnobacterium (C. piscicola and C. divergens), Aeromonas, and Photobacterium were the most common genera, and Lactococcus lactis, Lactobacillus plantarum, Pseudomonas fluorescens, and Aeromonas hydrophila were the most common species. These findings indicate that the microbiological quality of fresh fish in Tunisia can be preserved by controlling pathogenic and psychrotrophic bacteria.

  20. A fresh look at space solar power

    International Nuclear Information System (INIS)

    Mankins, J.C.

    1996-01-01

    Studies of systems to provide solar power from space for terrestrial use defined very large, geostationary Earth orbit (GEO) satellite concepts that--given massive initial government investments and extremely low cost space launch--might have led to power production at costs only somewhat higher than expected commercial prices. These studies of space solar power (SSP) succeeded in establishing technical feasibility. Shortly after the completion of the 1970s study, however, US funding came to an abrupt and seemingly permanent halt--in part because projected costs for the reference system were staggering: well in excess of $100B to achieve the first commercial kilowatt-hour of power. SSP has seen sporadic study and limited experimentation during the past decade (e.g., in Japan). Still, no existing SSP concept has engendered private development. New technologies now make possible concepts and approaches that suggest that SSP economic feasibility may be achievable early in the next century. In 1995, NASA's Advanced Concepts Office initiated a study taking a fresh look at innovative concepts for SSP that differ markedly from previously examined concepts, addressing innovative system architectures, markets and technologies that could radically reduce initial and operational costs. This paper will explore the issues associated with SSP and will summarize the results to date of NASA's recent fresh look at this important and increasingly timely field of space applications

  1. Isolated galaxies

    International Nuclear Information System (INIS)

    Einasto, Maret

    1990-01-01

    To test for the possible presence of really isolated galaxies, which form a randomly distributed population in voids, we compare the distribution of most isolated galaxies in an observed sample with distributions of the same number of random points using the nearest neighbour test. The results show that the random population of really isolated galaxies does not exist - even the most isolated galaxies are connected with systems of galaxies, forming their outlying parts. (author)

  2. Comparative biomechanical study between fresh frozen bone and fresh frozen pasteurized bone process

    International Nuclear Information System (INIS)

    Ferdiansyah Abdurrahman

    1999-01-01

    To observe the biomechanical properties difference between fresh frozen bone and fresh frozen pasteurized bone process Thirty eight femurs bones taken from 6 years old primate.(macaca fascicularis) from Primate Nursery Center LIPI Bogor, 20 bones were 6 cm cut for bending test and 18 remains were 3 cm cut for compression test. All bones were frozen and then divided into two groups for each biomechanical study. First group (I 0 bones for bending test and 9 bones for compression test) were undergone fresh frozen procession only. The second group with the same amount was undergone fresh frozen and pasteurized on 60 degree C for three hours. Bending test was done until the bones were broken on control group and pasteurized group and the result was compared, the same procedure was done for compression test. The study was done in room temperature. The biomechanical test result was analyzed by two independent T tests. The bending test control group has ( mean 0.097 N / mm sup 2 (SD = 0.007) and the pasteurized group ( mean 0. I 0 1 N / mm sup 2 (SD = 0.0 1 3), there was no significant difference (p 0.399). The compression test control group has ( = mean 0.71 N / mm sup 2 (SD=0.128)where as the pasteurized group has(mean 0.50N/mm sup 2 (SD=0.111),there was significant difference (p =0.004) From the result biomechanical study on bending test, there was no significant difference of bone strength, whereas on compression test the fresh frozen with pasteurized bone group is 125% stronger than control group. The result of this study will be very useful for reconstruction bone allograft

  3. Distribution of water in fresh cod

    DEFF Research Database (Denmark)

    Andersen, Charlotte Møller; Rinnan, A.

    2002-01-01

    Low-field (1)H nuclear magnetic resonance (NMR) transverse relaxation was used to measure water mobility and distribution of water in fresh cod fillets. The NMR relaxations were analysed with the so-called SLICING method giving uni-exponential profiles from which the transverse relaxation time (T(2......)-values) and the relative sizes of the water populations were calculated. Two water populations with the T(2)-values of 50 and 94 ms were obtained. The shortest relaxation time was primarily found near the head, and water with the longest relaxation time was primarily found near the tail. This variation...... can he explained by the smaller muscle cells and muscle fibers in the tail, which may influence the distributions of water into the different pools. The amount of one of the water populations was correlated to the overall water content with a correlation coefficient of -0.94. (C) 2002 Elsevier Science...

  4. Fresh perspectives for a better world

    CERN Multimedia

    Danielle Amy Venton

    While it’s no surprise that technology has the ability to change the world, it sometimes changes it in surprising ways. The Citizen Cyberscience Centre project is promoting change for humanitarian causes through distributed volunteer initiatives, such as volunteer computing and ‘volunteer thinking’. The inaugural lecture of a planned series was held at CERN in late October. The United Nations Institute for Training and Research (UNITAR), the University of Geneva (UNIGE), and CERN have recently set up a new partnership and launched a lecture series, which will invite experts in the humanities and technologies to share fresh perspectives on ways to work for a better world. The inaugural lecture at CERN was given by two speakers, Mo Ibrahim and Alpheus Bingham, each of them behind initiatives using technology to address difficult problems. Mo Ibrahim founded Celtel International, one of Africa’s most successful mobile network operators. Ibrahim’s company has ...

  5. Occurrence of Cronobacter spp. in Dried Foods, Fresh Vegetables and Soil.

    Science.gov (United States)

    Ueda, Shigeko

    2017-01-01

     The present study surveyed the occurrence of Cronobacter spp. in dried foods including milk powder, spices and herbs and others, and fresh vegetables commercially available in markets, and ground soil materials for the agriculture. Cronobacter spp. were isolated from 15% of 33 spice and herb samples and 3% of 36 taste foods, and these were C. turicensis, C. malonaticus, C. sakazakii and C. dubliensis. Cronobacter spp. from fresh vegetables were detected in 12% of field vegetables and 13% of hydroponic vegetables. C. turicensis was prevalent in field vegetables, and C. malonaticus was in hydroponic ones. And, Cronobacter spp. in shredded vegetables were detected from 44% of 9 samples, and these were C. dubliensis, C. turicensis and C. sakazakii. Also, Cronobacter spp. in soil from rice field, vegetable field and sandpits were predominantly C. sakazakii and C. malonaticus.

  6. Further observations on incorporation of the 14C-leucine into proteins by freshly secreted milk

    International Nuclear Information System (INIS)

    Singh, L.N.

    1976-01-01

    Using freshly secreted bovine milk, no incorporation of DL (1- 14 C)-leucine was observed in the total milk proteins and acid precipitated casein, when these protein fractions were isolated from skim milk. A significant portion of the radioactivity however, remained associated with the heat coagulable whey proteins and proteose-peptone fractions. This association was shown to be due to non enzymatic physical sequestering of the radioactive amino acid or its metabolites with these proteins. Most of the radioactivity was associated with the cream layer proteins and the cellular fraction. The results obtained using filtered milk, incubated milk and certain antibiotics also indicated that the incorporation of 14 C leucine into proteins by freshly secreted milk may be a purely microbial process and physical sequestering of an amino acids with milk proteins. (author)

  7. Electron beam processing of fresh produce - A critical review

    Science.gov (United States)

    Pillai, Suresh D.; Shayanfar, Shima

    2018-02-01

    To meet the increasing global demand for fresh produce, robust processing methods that ensures both the safety and quality of fresh produce are needed. Since fresh produce cannot withstand thermal processing conditions, most of common safety interventions used in other foods are ineffective. Electron beam (eBeam) is a non-thermal technology that can be used to extend the shelf life and ensure the microbiological safety of fresh produce. There have been studies documenting the application of eBeam to ensure both safety and quality in fresh produce, however, there are still unexplored areas that still need further research. This is a critical review on the current literature on the application of eBeam technology for fresh produce.

  8. Evaluation of Fresh Food Internet Shopping in Korean Beef

    OpenAIRE

    金, 鍾和; 森高, 正博; 福田, 晋; Kim, Jong-hwa; Moritaka, Masahiro; Fukuda, Susumu

    2013-01-01

    This paper focuses on consumer reactions to the fresh food internet shopping on Korea beef. In the paper, we have analyzed an effective relation in consumer's perceived qualities, the evaluation of commodities and the evaluation of fresh food internet shopping. As a result, two effective relations were found. Firstly, consumer's perceived qualities affect the evaluation of commodities. Secondly, the evaluation of commodities affects t-he evaluation of fresh food internet shopping. This result...

  9. The onshore influence of offshore fresh groundwater

    Science.gov (United States)

    Knight, Andrew C.; Werner, Adrian D.; Morgan, Leanne K.

    2018-06-01

    Freshwater contained within the submarine extensions of coastal aquifers is increasingly proposed as a freshwater source for coastal communities. However, the extent to which offshore freshwater supports onshore pumping is currently unknown on a global scale. This study provides the first attempt to examine the likely prevalence of situations where offshore freshwater influences onshore salinities, considering various sites from around the world. The groundwater conditions in twenty-seven confined and semi-confined coastal aquifers with plausible connections to inferred or observed offshore freshwater are explored. The investigation uses available onshore salinities and groundwater levels, and offshore salinity knowledge, in combination with analytical modelling, to develop simplified conceptual models of the study sites. Seven different conceptual models are proposed based on the freshwater-saltwater extent and insights gained from analytical modelling. We consider both present-day and pre-development conditions in assessing potential modern contributions to offshore fresh groundwater. Conceptual models also include interpretations of whether offshore freshwater is a significant factor influencing onshore salinities and well pumping sustainability. The results indicate that onshore water levels have declined between pre-development and present-day conditions in fourteen of the fifteen regions for which pre-development data are available. Estimates of the associated steady-state freshwater extents show the potential for considerable offshore fresh groundwater losses accompanying these declines. Both present-day and pre-development heads are insufficient to account for the observed offshore freshwater in all cases where adequate data exist. This suggests that paleo-freshwater and/or aquifer heterogeneities contribute significantly to offshore freshwater extent. Present-day heads indicate that active seawater intrusion (SWI) will eventually impact onshore pumping

  10. Pork loin quality is not indicative of fresh belly or fresh and cured ham quality.

    Science.gov (United States)

    Arkfeld, E K; Wilson, K B; Overholt, M F; Harsh, B N; Lowell, J E; Hogan, E K; Klehm, B J; Bohrer, B M; Mohrhauser, D A; King, D A; Wheeler, T L; Dilger, A C; Shackelford, S D; Boler, D D

    2016-12-01

    The objective was to characterize the relationship between fresh loin quality with fresh belly or fresh and cured ham quality. Pigs raised in 8 barns representing 2 seasons [cold ( = 4,290) and hot ( = 3,394)] and 2 production focuses [lean ( = 3,627) and quality ( = 4,057)] were used. Carcass characteristics and other meat quality data were collected on 7,684 carcasses. All of the carcasses were evaluated for HCW, LM depth, tenth rib fat depth, leg (ham primal) weight, instrumental color on the gluteus medius and gluteus profundus of the ham face, and subjective loin quality. Instrumental loin color and ultimate pH (≥ 22 h postmortem) were collected on the ventral side of loins along with dimensions and firmness scores of fresh bellies from 50% of the carcasses. Ten percent of the boneless loins and fresh hams were evaluated for slice shear force (SSF) or cured ham characteristics. Correlation coefficients between traits were computed using the CORR procedure of SAS and considered significantly different from 0 at ≤ 0.05. Temperature decline, beginning at 31 min postmortem and concluding at 22 h postmortem, for the longissimus dorsi and semimembranosus muscles were evaluated on 10% of the carcasses. Ultimate loin pH was correlated with dimensional belly characteristics ( ≥ |0.07|; ham instrumental color ( ≥ |0.03|; ≤ 0.05), and semimembranosus ultimate pH ( = 0.33; hams. Instrumental L*on the ventral surface of the loin was related to L* on both muscles of the ham face ( ≤ 0.0001). Even though significant relationships between the loin, belly, and ham were detected, the variability in belly and ham quality explained by variability in loin quality was poor (≤ 22.09%). Compositional differences between the loin and belly may have contributed to those poor relationships. Additionally, differences in temperature declines during chilling between the loin and ham likely contributed to the weak nature of relationships. Equilibration of longissimus dorsi

  11. Control of foodborne pathogens on fresh-cut fruit by a novel strain of Pseudomonas graminis.

    Science.gov (United States)

    Alegre, Isabel; Viñas, Inmaculada; Usall, Josep; Teixidó, Neus; Figge, Marian J; Abadias, Maribel

    2013-06-01

    The consumption of fresh-cut fruit has substantially risen over the last few years, leading to an increase in the number of outbreaks associated with fruit. Moreover, consumers are currently demanding wholesome, fresh-like, safe foods without added chemicals. As a response, the aim of this study was to determine if the naturally occurring microorganisms on fruit are "competitive with" or "antagonistic to" potentially encountered pathogens. Of the 97 and 107 isolates tested by co-inoculation with Escherichia coli O157:H7, Salmonella and Listeria innocua on fresh-cut apple and peach, respectively, and stored at 20 °C, seven showed a strong antagonistic capacity (more than 1-log unit reduction). One of the isolates, CPA-7, achieved the best reduction values (from 2.8 to 5.9-log units) and was the only isolate able to inhibit E. coli O157:H7 at refrigeration temperatures on both fruits. Therefore, CPA-7 was selected for further assays. Dose-response assays showed that CPA-7 should be present in at least the same amount as the pathogen to adequately reduce the numbers of the pathogen. From the results obtained in in vitro assays, competition seemed to be CPA-7's mode of action against E. coli O157:H7. The CPA-7 strain was identified as Pseudomonas graminis. Thus, the results support the potential use of CPA-7 as a bioprotective agent against foodborne pathogens in minimally processed fruit. Copyright © 2013 Elsevier Ltd. All rights reserved.

  12. Np Analysis of Freshly Separated Material

    International Nuclear Information System (INIS)

    Hodge, Christopher A.

    2004-01-01

    Np analysis has historically relied upon the Pa/Np secular equilibrium for quantitative results. The Savannah River Site has recently undertaken a mission concerning Np. In this application, Np is separated from Pa, packaged, possibly mixed with other batches, and assayed. The fresh separation and mixing of batches prevents the use of the Pa133 prodigy peaks for assay. This effort was a developmental project novel in the DOE complex. This paper discusses the challenges encountered in the design of instrumentation to assay Np directly. The most abundant Np peak that is unfettered by Pa prodigy, Am, or other SNM materials is the 29.6keV. Quantitative measurement of such a low energy peak presents significant problems with attenuation, Compton effects, and other such issues that arise while trying to maximize the effects of low energy peaks. The solutions rely on exploiting the ''tried and true'' methods with regard to shielding, detector technology, and calibration techniques, but wit h a very low energy perspective. The results are intriguing and, with some assumptions regarding sample homogeneity, quantitative within normal MC and A statistics

  13. Two new monoterpenoid glycosides from the fresh rhizome of Tongling White Ginger (Zingiber officinale).

    Science.gov (United States)

    Guo, Tao; Tan, Su-Bei; Wang, Ya; Chang, Jun

    2018-01-01

    Two new monoterpenoid glycosides, trans-1,8-cineole-3,6-dihydroxy-3-O-β-D-glucopyranoside (1), and 5,9-dihydroxy borneol 2-O-β-D-glucopyranoside (2), together with four known monoterpenoid glycosides (3-6), were isolated from the water-soluble constituents of the fresh rhizome of Tongling White Ginger (Zingiber officinale). Their structures were decisively elucidated by spectroscopic analysis. In vitro tests for antimicrobial activity showed that compounds 1 and 3 possess significant activity against two Gram-positive organisms, Staphylococcus aureus and Staphylococcus epidermidis.

  14. Agrifood systems and the microbial safety of fresh produce: Trade-offs in the wake of increased sustainability

    International Nuclear Information System (INIS)

    Nguyen-the, Christophe; Bardin, Marc; Berard, Annette; Berge, Odile; Brillard, Julien; Broussolle, Véronique

    2016-01-01

    Fresh produce has been a growing cause of food borne outbreaks world-wide prompting the need for safer production practices. Yet fresh produce agrifood systems are diverse and under constraints for more sustainability. We analyze how measures taken to guarantee safety interact with other objectives for sustainability, in light of the diversity of fresh produce agrifood systems. The review is based on the publications at the interface between fresh produce safety and sustainability, with sustainability defined by low environmental impacts, food and nutrition security and healthy life. The paths for more sustainable fresh produce are diverse. They include an increased use of ecosystem services to e.g. favor predators of pests, or to reduce impact of floods, to reduce soil erosion, or to purify run-off waters. In contrast, they also include production systems isolated from the environment. From a socio-economical view, sustainability may imply maintaining small tenures with a higher risk of pathogen contamination. We analyzed the consequences for produce safety by focusing on risks of contamination by water, soil, environment and live stocks. Climate change may increase the constraints and recent knowledge on interactions between produce and human pathogens may bring new solutions. Existing technologies may suffice to resolve some conflicts between ensuring safety of fresh produce and moving towards more sustainability. However, socio-economic constraints of some agri-food systems may prevent their implementation. In addition, current strategies to preserve produce safety are not adapted to systems relying on ecological principles and knowledge is lacking to develop the new risk management approaches that would be needed. - Highlights: • Measures taken to improve safety were assessed for their impact on sustainability. • Fresh produce safety improvements may come at the expense of sustainability. • Environment, food security and human health constituted the

  15. Use of Irradiation to Ensure Microbial Safety of Fresh-Cut Green Beans Sold In Egyptian Supermarkets

    International Nuclear Information System (INIS)

    Abu El-Nour, S.A.

    2010-01-01

    Thirty samples of fresh-cut green beans were collected from different local supermarkets. They were tested for their microbiological quality. Total aerobic plate count (TAPC) of these samples ranged from >105 to 108 cfu/g; and total yeasts and moulds ranged from 102 to 105 cfu/g. All tested fresh-cut green beans samples were contaminated with E. coli. Only 6 samples were free from Enterococcus faecalis, the other 24 (80%) samples contained Ent. faecalis in the range of 102 to 104 cfu/g. Staphylococcus aureus was found in 20 samples (66.6 %) at level of 102 to 104 cfu/g. Aeromonas hydrophila was found in 22 (73.3 %) samples in the range of 102 to 103 cfu/g. All tested fresh cut green beans samples were free from Listeria monocytogenes and Salmonella spp. The D10-values of the isolated pathogenic bacteria (Ent. faecalis, Staph. aureus, E. coli and A. hydrophila) in fresh-cut green beans were found to be 0.58, 0.42, 0.23 and 0.11, respectively. Fresh-cut green beans samples were irradiated at 1.5 and 3.0 kGy, and then stored at refrigeration temperature (4 degree C ± 1). Generally, these radiation doses greatly reduced all microbial counts of fresh-cut green beans. The percentage of reduction reached 97.07 and 99.88 in TAPC. Irradiation dose of 1.5 kGy was enough for complete elimination of E. coli and A. hydrophila but was not sufficient for elimination of Ent. faecalis, and Staph. aureus. Irradiation dose of 3.0 kGy completely eliminated Ent. faecalis, and Staph. aureus present. This irradiation dose had no effect on sensorial quality attributes of fresh-cut green beans samples and extended the shelf-life to 15 days against only 5 days for unirradiated samples

  16. Agrifood systems and the microbial safety of fresh produce: Trade-offs in the wake of increased sustainability

    Energy Technology Data Exchange (ETDEWEB)

    Nguyen-the, Christophe, E-mail: christophe.nguyen-the@avignon.inra.fr [UMR408 SQPOV «Sécurité et Qualité des Produits d' Origine Végétale», INRA, Avignon Université, 84000 Avignon (France); Bardin, Marc, E-mail: marc.bardin@avignon.inra.fr [INRA, UR0407 Plant Pathology, F-84143 Montfavet (France); Berard, Annette, E-mail: annette.berard@avignon.inra.fr [EMMAH, INRA, Avignon Université, 84000 Avignon (France); Berge, Odile, E-mail: odile.berge@avignon.inra.fr [INRA, UR0407 Plant Pathology, F-84143 Montfavet (France); Brillard, Julien, E-mail: julien.brillard@univ-montp2.fr [UMR408 SQPOV «Sécurité et Qualité des Produits d' Origine Végétale», INRA, Avignon Université, 84000 Avignon (France); Broussolle, Véronique, E-mail: veronique.broussolle@avignon.inra.fr [UMR408 SQPOV «Sécurité et Qualité des Produits d' Origine Végétale», INRA, Avignon Université, 84000 Avignon (France); and others

    2016-08-15

    Fresh produce has been a growing cause of food borne outbreaks world-wide prompting the need for safer production practices. Yet fresh produce agrifood systems are diverse and under constraints for more sustainability. We analyze how measures taken to guarantee safety interact with other objectives for sustainability, in light of the diversity of fresh produce agrifood systems. The review is based on the publications at the interface between fresh produce safety and sustainability, with sustainability defined by low environmental impacts, food and nutrition security and healthy life. The paths for more sustainable fresh produce are diverse. They include an increased use of ecosystem services to e.g. favor predators of pests, or to reduce impact of floods, to reduce soil erosion, or to purify run-off waters. In contrast, they also include production systems isolated from the environment. From a socio-economical view, sustainability may imply maintaining small tenures with a higher risk of pathogen contamination. We analyzed the consequences for produce safety by focusing on risks of contamination by water, soil, environment and live stocks. Climate change may increase the constraints and recent knowledge on interactions between produce and human pathogens may bring new solutions. Existing technologies may suffice to resolve some conflicts between ensuring safety of fresh produce and moving towards more sustainability. However, socio-economic constraints of some agri-food systems may prevent their implementation. In addition, current strategies to preserve produce safety are not adapted to systems relying on ecological principles and knowledge is lacking to develop the new risk management approaches that would be needed. - Highlights: • Measures taken to improve safety were assessed for their impact on sustainability. • Fresh produce safety improvements may come at the expense of sustainability. • Environment, food security and human health constituted the

  17. Preliminary Phytochemical Screening of Fresh and Dried Moringa ...

    African Journals Online (AJOL)

    The preliminary phytochemical screening of the crude aqueous extract of fresh and dried Moringa oleifera leaves, of the organic solvent and residual fractions of the extract was carried out according to the standard methods. Chemical constituents of the crude aqueous extract of the fresh leaves were found to be tannins, ...

  18. Phytosanitary irradiation and fresh fruit quality: Cultivar and maturity effects

    Science.gov (United States)

    Irradiation is an effective quarantine treatment for global trade of fresh produce. Variation in cultivars and maturity stages can impact the tolerance of fresh fruits to irradiation for the purposes of quarantine security. Tolerance thresholds for irradiated fruit are lacking for a large number of ...

  19. Rumen dry matter degradability of fresh and ensiled sugarcane ...

    African Journals Online (AJOL)

    This study aimed to evaluate the chemical composition and in situ ruminal degradability of fresh (FSC) and ensiled (ESC) sugarcane. In situ dry matter degradability (DMD) was determined using the nylon bag technique with four cows equipped ruminal fistulas. Cows were fed with fresh or ensiled sugarcane and ...

  20. Robust model of fresh jujube soluble solids content with near ...

    African Journals Online (AJOL)

    A robust partial least square (PLS) calibration model with high accuracy and stability was established for the measurement of soluble solids content (SSC) of fresh jujube using near-infrared (NIR) spectroscopy technique. Fresh jujube samples were collected in different areas of Taigu and Taiyuan cities, central China in ...

  1. 9 CFR 319.141 - Fresh pork sausage.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Fresh pork sausage. 319.141 Section... AGENCY ORGANIZATION AND TERMINOLOGY; MANDATORY MEAT AND POULTRY PRODUCTS INSPECTION AND VOLUNTARY INSPECTION AND CERTIFICATION DEFINITIONS AND STANDARDS OF IDENTITY OR COMPOSITION Sausage Generally: Fresh...

  2. Improving the safety of fresh fruit and vegetables

    NARCIS (Netherlands)

    Jongen, W.M.F.

    2005-01-01

    Fresh fruit and vegetables have been identified as a significant source of pathogens and chemical contaminants. As a result, there has been a wealth of research on identifying and controlling hazards at all stages in the supply chain. Improving the safety of fresh fruit and vegetables reviews this

  3. 78 FR 6227 - Importation of Fresh Apricots From Continental Spain

    Science.gov (United States)

    2013-01-30

    .... APHIS-2011-0132] RIN 0579-AD62 Importation of Fresh Apricots From Continental Spain AGENCY: Animal and... continental Spain. As a condition of entry, fresh apricots from continental Spain would have to be produced in... organization of Spain certifying that the fruit is free from all quarantine pests and has been produced in...

  4. Incidence of staphylococcus aureus in locally produced fresh milk ...

    African Journals Online (AJOL)

    This paper investigates the incidence of the bacterial organism Staphylococcus, aureus in locally produced fresh milk (nono). The fresh milk was obtained from the Damaturu main market, Yobe state of Nigeria. Petri dishes were washed and allowed to dry. They were then sterilized in hot air oven at 130°C for two hours and ...

  5. Creating Sustainable Fresh Food Supply Chains through Waste Reduction

    DEFF Research Database (Denmark)

    Kaipia, Riikka; Dukovska-Popovska, Iskra; Loikkanen, Lauri

    2013-01-01

    . Design/methodology/approach – This work has been designed as an exploratory case study in three fresh food supply chains, milk, fresh fish, and fresh poultry, in the Nordic countries. The cases are based on interviews and data from the databases of the companies involved. Each case focuses on analyzing...... uses of shared information to create a sustainable fresh food supply chain. Findings –The performance of the perishable food chain can be improved by more efficient information sharing. The key to improved operations is how and for which purposes the shared data should be used. In addition, changes......Purpose – The aim of this empirical paper is to study information sharing in fresh food supply chains, with a specific goal of reducing waste and facilitating sustainable performance. The study focuses on material and information flow issues, specifically on sharing demand and shelf-life data...

  6. Smart storage technologies applied to fresh foods: A review.

    Science.gov (United States)

    Wang, Jingyu; Zhang, Min; Gao, Zhongxue; Adhikari, Benu

    2017-06-30

    Fresh foods are perishable, seasonal and regional in nature and their storage, transportation, and preservation of freshness are quite challenging. Smart storage technologies can online detection and monitor the changes of quality parameters and storage environment of fresh foods during storage, so that operators can make timely adjustments to reduce the loss. This article reviews the smart storage technologies from two aspects: online detection technologies and smartly monitoring technologies for fresh foods. Online detection technologies include electronic nose, nuclear magnetic resonance (NMR), near infrared spectroscopy (NIRS), hyperspectral imaging and computer vision. Smartly monitoring technologies mainly include some intelligent indicators for monitoring the change of storage environment. Smart storage technologies applied to fresh foods need to be highly efficient and nondestructive and need to be competitively priced. In this work, we have critically reviewed the principles, applications, and development trends of smart storage technologies.

  7. Polyphenolics free DNA isolation and optimization of PCR-RAPD for ...

    African Journals Online (AJOL)

    , and good quality high molecular weight DNA samples. DNA was isolated from the mature and fresh young tender leaves obtained from various Italian wild populations of fennel. We performed a modified cetyl trimethyl ammonium bromide ...

  8. Shelf Life Determination of Fresh Blueberries (Vaccinium corymbosum Stored under Controlled Atmosphere and Ozone

    Directory of Open Access Journals (Sweden)

    Anibal Concha-Meyer

    2015-01-01

    Full Text Available Fresh blueberries are commonly stored and transported by refrigeration in controlled atmospheres to protect shelf life for long periods of storage. Ozone is an antimicrobial gas that can extend shelf life and protect fruit from microbial contamination. Shelf life of fresh highbush blueberries was determined over 10-day storage in isolated cabinets at 4°C or 12°C under different atmosphere conditions, including air (control; 5% O2 : 15% CO2 : 80% N2 (controlled atmosphere storage (CAS; and ozone gas (O3 4 ppm at 4°C or 2.5 ppm at 12°C, at high relative humidity (90–95%. Samples were evaluated for yeast and molds growth, weight loss, and firmness. CAS and O3 did not delay or inhibit yeast and molds growth in blueberries after 10 days at both temperatures. Fruit stored at 4°C showed lower weight loss values compared with 12°C. Blueberries stored under O3 atmosphere showed reduced weight loss at 12°C by day 10 and loss of firmness when compared to the other treatments. Low concentrations of ozone gas together with proper refrigeration temperature can help protect fresh blueberries quality during storage.

  9. Shelf Life Determination of Fresh Blueberries (Vaccinium corymbosum) Stored under Controlled Atmosphere and Ozone.

    Science.gov (United States)

    Concha-Meyer, Anibal; Eifert, Joseph D; Williams, Robert C; Marcy, Joseph E; Welbaum, Gregory E

    2015-01-01

    Fresh blueberries are commonly stored and transported by refrigeration in controlled atmospheres to protect shelf life for long periods of storage. Ozone is an antimicrobial gas that can extend shelf life and protect fruit from microbial contamination. Shelf life of fresh highbush blueberries was determined over 10-day storage in isolated cabinets at 4°C or 12°C under different atmosphere conditions, including air (control); 5% O2 : 15% CO2 : 80% N2 (controlled atmosphere storage (CAS)); and ozone gas (O3) 4 ppm at 4°C or 2.5 ppm at 12°C, at high relative humidity (90-95%). Samples were evaluated for yeast and molds growth, weight loss, and firmness. CAS and O3 did not delay or inhibit yeast and molds growth in blueberries after 10 days at both temperatures. Fruit stored at 4°C showed lower weight loss values compared with 12°C. Blueberries stored under O3 atmosphere showed reduced weight loss at 12°C by day 10 and loss of firmness when compared to the other treatments. Low concentrations of ozone gas together with proper refrigeration temperature can help protect fresh blueberries quality during storage.

  10. Identification of innovative potential quality markers in rocket and melon fresh-cut produce.

    Science.gov (United States)

    Cavaiuolo, Marina; Cocetta, Giacomo; Bulgari, Roberta; Spinardi, Anna; Ferrante, Antonio

    2015-12-01

    Ready-to-eat fresh cut produce are exposed to pre- and postharvest abiotic stresses during the production chain. Our work aimed to identify stress responsive genes as new molecular markers of quality that can be widely applied to leaves and fruits and easily determined at any stage of the production chain. Stress responsive genes associated with quality losses were isolated in rocket and melon fresh-cut produce and their expression levels analyzed by quantitative real time PCR (qRT-PCR) at different time points after harvest at 20 °C and 4 °C. qRT-PCR results were supported by correlation analysis with physiological and biochemical determinations evaluated at the same conditions such as chlorophyll a fluorescence indices, total, reducing sugars, sucrose, ethylene, ascorbic acid, lipid peroxidation and reactive oxygen species. In both species the putative molecular markers increased their expression soon after harvest suggesting a possible use as novel and objective quality markers of fresh-cut produces. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Antimicrobial edible films and coatings for fresh and minimally processed fruits and vegetables: a review.

    Science.gov (United States)

    Valencia-Chamorro, Silvia A; Palou, Lluís; Del Río, Miguel A; Pérez-Gago, María B

    2011-01-01

    The use of edible films and coatings is an environmentally friendly technology that offers substantial advantages for shelf-life increase of many food products including fruits and vegetables. The development of new natural edible films and coatings with the addition of antimicrobial compounds to preserve fresh and minimally processed fruits and vegetables is a technological challenge for the industry and a very active research field worldwide. Antimicrobial agents have been successfully added to edible composite films and coatings based on polysaccharides or proteins such as starch, cellulose derivatives, chitosan, alginate, fruit puree, whey protein isolated, soy protein, egg albumen, wheat gluten, or sodium caseinate. This paper reviews the development of edible films and coatings with antimicrobial activity, typically through the incorporation of antimicrobial food additives as ingredients, the effect of these edible films on the control of target microorganisms, the influence of antimicrobial agents on mechanical and barrier properties of stand-alone edible films, and the effect of the application of antimicrobial edible coatings on the quality of fresh and fresh-cut fruits and vegetables.

  12. Isolation of mesenchymal stem cells from equine umbilical cord blood

    DEFF Research Database (Denmark)

    Koch, Thomas Gadegaard; Heerkens, Tammy; Thomsen, Preben Dybdahl

    2007-01-01

    . The hypothesis of this study was that equine MSCs could be isolated from fresh whole equine cord blood. Results: Cord blood was collected from 7 foals immediately after foaling. The mononuclear cell fraction was isolated by Ficoll density centrifugation and cultured in a DMEM low glucose based media at 38.5o......Background: There are no published studies on stem cells from equine cord blood although commercial storage of equine cord blood for future autologous stem cell transplantations is available. Mesenchymal stem cells (MSC) have been isolated from fresh umbilical cord blood of humans collected non......-invasively at the time of birth and from sheep cord blood collected invasively by a surgical intrauterine approach. Mesenchymal stem cells isolation percentage from frozen-thawed human cord blood is low and the future isolation percentage of MSCs from cryopreserved equine cord blood is therefore expectedly low...

  13. Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource

    OpenAIRE

    Srivastava, Akanksha; Tiwari, Ratnakar; Srivastava, Vikas; Singh, Tej Bali; Asthana, Ravi Kumar

    2015-01-01

    An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732) were isolated (paddy fields and ponds in the Banaras Hindu University, campus) and five strains screened for ant...

  14. Enterobacteriaceae resistant to third-generation cephalosporins and quinolones in fresh culinary herbs imported from Southeast Asia.

    Science.gov (United States)

    Veldman, Kees; Kant, Arie; Dierikx, Cindy; van Essen-Zandbergen, Alieda; Wit, Ben; Mevius, Dik

    2014-05-02

    Since multidrug resistant bacteria are frequently reported from Southeast Asia, our study focused on the occurrence of ESBL-producing Enterobacteriaceae in fresh imported herbs from Thailand, Vietnam and Malaysia. Samples were collected from fresh culinary herbs imported from Southeast Asia in which ESBL-suspected isolates were obtained by selective culturing. Analysis included identification by MALDI-TOF mass spectrometry, susceptibility testing, XbaI-PFGE, microarray, PCR and sequencing of specific ESBL genes, PCR based replicon typing (PBRT) of plasmids and Southern blot hybridization. In addition, the quinolone resistance genotype was characterized by screening for plasmid mediated quinolone resistance (PMQR) genes and mutations in the quinolone resistance determining region (QRDR) of gyrA and parC. The study encompassed fifty samples of ten batches of culinary herbs (5 samples per batch) comprising nine different herb variants. The herbs originated from Thailand (Water morning glory, Acacia and Betel leaf), Vietnam (Parsley, Asian pennywort, Houttuynia leaf and Mint) and Malaysia (Holy basil and Parsley). By selective culturing 21 cefotaxime resistant Enterobacteriaceae were retrieved. Array analysis revealed 18 isolates with ESBL genes and one isolate with solely non-ESBL beta-lactamase genes. Mutations in the ampC promoter region were determined in two isolates with PCR and sequencing. The isolates were identified as Klebsiella pneumoniae (n=9), Escherichia coli (n=6), Enterobacter cloacae complex (n=5) and Enterobacter spp. (n=1). All isolates tested were multidrug resistant. Variants of CTX-M enzymes were predominantly found followed by SHV enzymes. PMQR genes (including aac(6')-1b-cr, qnrB and qnrS) were also frequently detected. In almost all cases ESBL and quinolone resistance genes were located on the same plasmid. Imported fresh culinary herbs from Southeast Asia are a potential source for contamination of food with multidrug resistant bacteria

  15. Listeria monocytogenes in Fresh Produce: Outbreaks, Prevalence and Contamination Levels

    Directory of Open Access Journals (Sweden)

    Qi Zhu

    2017-03-01

    Full Text Available Listeria monocytogenes, a member of the genus Listeria, is widely distributed in agricultural environments, such as soil, manure and water. This organism is a recognized foodborne pathogenic bacterium that causes many diseases, from mild gastroenteritis to severe blood and/or central nervous system infections, as well as abortion in pregnant women. Generally, processed ready-to-eat and cold-stored meat and dairy products are considered high-risk foods for L. monocytogenes infections that cause human illness (listeriosis. However, recently, several listeriosis outbreaks have been linked to fresh produce contamination around the world. Additionally, many studies have detected L. monocytogenes in fresh produce samples and even in some minimally processed vegetables. Thus L. monocytogenes may contaminate fresh produce if present in the growing environment (soil and water. Prevention of biofilm formation is an important control measure to reduce the prevalence and survival of L. monocytogenes in growing environments and on fresh produce. This article specifically focuses on fresh produce–associated listeriosis outbreaks, prevalence in growing environments, contamination levels of fresh produce, and associated fresh produce safety challenges.

  16. Survival of Listeria monocytogenes on fresh blueberries (Vaccinium corymbosum) stored under controlled atmosphere and ozone.

    Science.gov (United States)

    Concha-Meyer, Anibal; Eifert, Joseph; Williams, Robert; Marcy, Joseph; Welbaum, Gregory

    2014-05-01

    Listeria monocytogenes is a foodborne pathogen that represents a high risk for consumers because it can grow under refrigeration conditions and can also develop acid tolerance. Fresh blueberries are hand-picked, packed, and transported under refrigeration without receiving a microbial inactivation treatment. The aim of this work was to study the survival of L. monocytogenes in fresh highbush blueberries stored at 4 or 12 °C under different controlled atmosphere conditions, including air (control); 5% O2, 15% CO2, 80% N2 (controlled atmosphere storage [CAS]); or ozone gas (O3), 4 ppm at 4 °C or 2.5 ppm at 12 °C, at high relative humidity (90 to 95%) for a total of 10 days. Fresh blueberries inside a plastic clamshell were spot inoculated with the bacteria and were stored at 4 or 12 °C in isolated cabinets under air, CAS, and O3 atmospheric conditions. Samples were evaluated on days 0, 1, 4, 7, and 10 for microbial growth using modified Oxford agar. CAS did not delay or inhibit L. monocytogenes growth in fresh blueberries after 10 days. O3 achieved 3- and 2-log reductions when compared with air treatment at 4 and 12 °C, respectively. Low concentrations of O3 together with proper refrigeration temperature can ensure product safety throughout transportation. O3 is a strong antimicrobial that safely decomposes to oxygen and water without leaving residues and can be used as an alternative method to prevent bacterial growth during a long transport period.

  17. Cryopreservation and distribution of radiation-attenuated helminth larvae and the use of radioisotopes to monitor their survival. Coordinated programme on preparation of irradiated vaccines against some human diseases

    International Nuclear Information System (INIS)

    James, E.

    1982-06-01

    Techniques for the cryopreservation of schistosomula are described, from the methanol/two-step cooling technique, through a technique which uses 40% methanol and rapid cooling to the current technique which employs a two-step addition of ethanediol and rapid cooling. Levels of survival with these techniques have improved from 0.3% to 5.9% and now to 47% of control values. The 40% methanol/rapid cooling technique is described in detail as this forms the basis for understanding the role of cryoprotective additives and cooling and warming rates in the cryopreservation of schistosomula. The toxicity of 12 different potentially cryoprotective compounds is described. Cryopreservation of S.japonicum and S.bovis is described. The effect of the age of the schistosomula and their cryopreservability is related to the development of water sensitivity and the permeation and damage produced by glycerol and it is postulated that morphological changes occurring in the tegument during transformation from a cercaria to schistosomulum may account for these observations. Studies with 14 C-ethanediol are described which attempt to provide an understanding of permeability of cryoprtectants to schistosomula of different ages and at different temperatures. Vaccination studies with cryopreserved and radiation-attenuated schistosomula are also reported. Radiation-attenuated schistosomula are also reported. Radiation-attenuated cercariae and schistosomula produced high (64% to 89%) levels of protection in baboons, and cryopreserved schistosomula produced comparable levels of protection in vaccinated mice to normal schistosomula. Cryopreserved radiation-attenuated schistosomula produced a significant level of protection (49% reduction) in sheep although the numbers of normally motile organisms injected was low (1,000 per dose, 2 doses). It is concluded that normally-motile cryopreserved radiation-attenuated schistosomula are as immunogenic as fresh organisms

  18. An ESR study of radicals induced in irradiated fresh mango

    International Nuclear Information System (INIS)

    Kikuchi, Masahiro; Hussain, Mohammed S.; Morishita, Norio; Kobayashi, Yasuhiko; Ukai, Mitsuko; Shimoyama, Yuhei

    2009-01-01

    An electron spin resonance (ESR) spectroscopic study was performed on the radicals induced irradiated fresh mangoes. Fresh Philippine mangoes were irradiated by the γ-rays, lyophilized and powdered. The ESR spectrum of the dry specimen showed a strong main peak at g=2.004 and a pair of peaks at both magnetic fields of the main peak. The main peak detected from flesh and skin specimens faded away in a few days after the irradiation. On the other hand, the side peaks showed a well-defined dose response even 9 days after the irradiation. The side-peak is a useful mean to define the irradiation on fresh mangoes. (author)

  19. Occurrence of Natural Bacillus thuringiensis Contaminants and Residues of Bacillus thuringiensis-Based Insecticides on Fresh Fruits and Vegetables

    Science.gov (United States)

    Frederiksen, Kristine; Rosenquist, Hanne; Jørgensen, Kirsten; Wilcks, Andrea

    2006-01-01

    A total of 128 Bacillus cereus-like strains isolated from fresh fruits and vegetables for sale in retail shops in Denmark were characterized. Of these strains, 39% (50/128) were classified as Bacillus thuringiensis on the basis of their content of cry genes determined by PCR or crystal proteins visualized by microscopy. Random amplified polymorphic DNA analysis and plasmid profiling indicated that 23 of the 50 B. thuringiensis strains were of the same subtype as B. thuringiensis strains used as commercial bioinsecticides. Fourteen isolates were indistinguishable from B. thuringiensis subsp. kurstaki HD1 present in the products Dipel, Biobit, and Foray, and nine isolates grouped with B. thuringiensis subsp. aizawai present in Turex. The commercial strains were primarily isolated from samples of tomatoes, cucumbers, and peppers. A multiplex PCR method was developed to simultaneously detect all three genes in the enterotoxin hemolysin BL (HBL) and the nonhemolytic enterotoxin (NHE), respectively. This revealed that the frequency of these enterotoxin genes was higher among the strains indistinguishable from the commercial strains than among the other B. thuringiensis and B. cereus-like strains isolated from fruits and vegetables. The same was seen for a third enterotoxin, CytK. In conclusion, the present study strongly indicates that residues of B. thuringiensis-based insecticides can be found on fresh fruits and vegetables and that these are potentially enterotoxigenic. PMID:16672488

  20. Development of a new lactic acid bacterial inoculant for fresh rice straw silage

    Directory of Open Access Journals (Sweden)

    Jong Geun Kim

    2017-07-01

    Full Text Available Objective Effects of newly isolated Lactobacillus plantarum on the fermentation and chemical composition of fresh rice straw silage was evaluated in this study. Methods Lactic acid bacteria (LAB from good crop silage were screened by growing them in MRS broth and a minimal medium with low carbohydrate content. Selected LAB (LAB 1821 were Gram-positive, rods, catalase negative, and were identified to be Lactobacillus plantarum based on their biochemical characteristics and a 16S rRNA analysis. Fresh rice straw was ensiled with two isolated LAB (1821 and 1841, two commercial inoculants (HM/F and P1132 and no additive as a control. Results After 2 months of storage at ambient temperature, rice straw silages treated with additives were well-preserved, the pH values and butyric and acetic acid contents were lower, and the lactic acid content and lactic/acetic acid ratio were higher than those in the control (p0.05 effect on acid detergent fiber or neutral detergent fiber contents. Crude protein (CP content and in vitro DM digestibility (IVDMD increased after inoculation of LAB 1821 (p<0.05. Conclusion LAB 1821 increased the CP, IVDMD, lactic acid content and ratio of lactic acid to acetic acid in rice straw silage and decreased the pH, acetic acid, NH3-N, and butyric acid contents. Therefore, adding LAB 1821 improved the fermentation quality and feed value of rice straw silage.

  1. sensory analysis of cooked fresh meat sausages containing beef offal

    African Journals Online (AJOL)

    user

    Sensory analysis of cooked fresh meat sausages containing beef offal. 22 .... Trained and consumer pan- els from the local black ... selected as the best formulations or recipes, as judged by the ... loosening of the sausage from the pan with a.

  2. Effect of fresh air ventilation on indoor radon concentration

    International Nuclear Information System (INIS)

    Sun Hao; Wu Jianhua; Fu Shi

    2012-01-01

    The radon concentration of laboratory for radon simulation (LRS) was measured by the RAD7 radon monitor, and the effect of the different fresh air ventilations on indoor radon concentration was studied and analyzed. The indoor radon concentration of LRS can be accumulated up to 2000 Bq/m 3 and the average radon exhalation rate of the LRS is 14.5 Bq · m -2 . h -1 . Furthermore, when the fresh air enters into the LRS continuously, the indoor radon concentration decreases exponentially with the increase of time. The equilibrium radon concentration and equilibrium time of LRS decrease exponentially with the increase of the rate of fresh air ventilation. In addition, the indoor radon concentration increases by accumulation with the decrease of the rate of fresh air ventilation. (authors)

  3. In Vivo Digestibility of Molasses-Treated Fresh Banana Leaves ...

    African Journals Online (AJOL)

    treated fresh banana leaves in West African Dwarf sheep was conducted at the Teaching and Research Farm and Animal Nutrition Laboratory of the University of Dschang between August and September 2009. For this, six sheep were used and ...

  4. Green and technical efficient growth in Danish fresh water aquaculture

    DEFF Research Database (Denmark)

    Nielsen, Rasmus

    2011-01-01

    growth can be achieved by introducing new environmentally friendly water purification systems in Danish fresh water aquaculture. Data Envelopment Analysis is used to investigate whether different water purification systems and farm size influence technical efficiency. The empirical results indicate...

  5. Application of Ultrasonic Waves on Maintaining Freshness of Tilapia Fillet

    Directory of Open Access Journals (Sweden)

    Ruddy Suwandi

    2015-06-01

    Full Text Available ish fillet is one of fisheries products that easily deteriorated; hence handling techniques are needed to maintain the freshness. Ultrasonic wave have been widely applied to some of food products for maintaining freshness through microbial inactivation, however the ultrasonic application to fisheries products has not been reported. The purpose of this study was to analyze the effect of ultrasonic wave on fish freshness. The stages of the study were sample preparation, sonication, freshness parameters examination and histology observation. Ultrasonic wave did not affectthe organoleptic value and the TVB, but affected the pH value and the TPC. The sample in which the TPC value was found significantly different, were further observed after 48 and 96 hours storage. The result showed that the TPC value of sonicated sample for 9 minutes was lower to that of without sonication. Histology analysis showed, however, sonication made the structure of muscle fiber less compact and deformation of myomer was found.

  6. Chemical qualities of oils from some fresh and market vegetable ...

    African Journals Online (AJOL)

    JTEkanem

    production was examined by evaluating the oil yield and chemical qualities of oil extracted from fresh ... oil may be considered as Nigeria potential asset for biofuel and oleochemical production. Keywords: ..... standards for edible Arachis oil.

  7. Socioeconomic Determinants of Income From Fresh and Processed ...

    African Journals Online (AJOL)

    A total sample size of 84 crayfish marketers were in all randomly selected across three major Cray ... determine the income derived from fresh crayfish marketing while household size and purchasing ... EMAIL FULL TEXT EMAIL FULL TEXT

  8. Physicochemical changes in minimal ozone-treated fresh shrimp ...

    African Journals Online (AJOL)

    treated fresh shrimp were evaluated tandem with microbiological efficacy of treatment during iced storage of up to 10 days. Safely discharged from commercially available domestic-type ozone facility, a previously defined minimal ozone treatment ...

  9. Analysis of fresh maize marketing in Anambra state, Nigeria ...

    African Journals Online (AJOL)

    Analysis of fresh maize marketing in Anambra state, Nigeria. ... on marketing income in Anambra State, this study utilized descriptive statistic, Sherpherd-futrel model of determining marketing efficiency ... EMAIL FULL TEXT EMAIL FULL TEXT

  10. Wrinkle Ridges and Young Fresh Crater

    Science.gov (United States)

    2002-01-01

    (Released 10 May 2002) The Science Wrinkle ridges are a very common landform on Mars, Mercury, Venus, and the Moon. These ridges are linear to arcuate asymmetric topographic highs commonly found on smooth plains. The origin of wrinkle ridges is not certain and two leading hypotheses have been put forth by scientists over the past 40 years. The volcanic model calls for the extrusion of high viscosity lavas along linear conduits. This thick lava accumulated over these conduits and formed the ridges. The other model is tectonic and advocates that the ridges are formed by compressional faulting and folding. Today's THEMIS image is of the ridged plains of Lunae Planum located between Kasei Valles and Valles Marineris in the northern hemisphere of the planet. Wrinkle ridges are found mostly along the eastern side of the image. The broadest wrinkle ridges in this image are up to 2 km wide. A 3 km diameter young fresh crater is located near the bottom of the image. The crater's ejecta blanket is also clearly seen surrounding the sharp well-defined crater rim. These features are indicative of a very young crater that has not been subjected to erosional processes. The Story The great thing about the solar system is that planets are both alike and different. They're all foreign enough to be mysterious and intriguing, and yet familiar enough to be seen as planetary 'cousins.' By comparing them, we can learn a lot about how planets form and then evolve geologically over time. Crinkled over smooth plains, the long, wavy raised landforms seen here are called 'wrinkle ridges,' and they've been found on Mars, Mercury, Venus, and the Moon - that is, on rocky bodies that are a part of our inner solar system. We know from this observation that planets (and large-enough moons) follow similar processes. What we don't know for sure is HOW these processes work. Scientists have been trying to understand how wrinkle ridges form for 40 years, and they still haven't reached a conclusion. That

  11. Meat and Fish Freshness Inspection System Based on Odor Sensing

    OpenAIRE

    Hasan, Najam ul; Ejaz, Naveed; Ejaz, Waleed; Kim, Hyung Seok

    2012-01-01

    We propose a method for building a simple electronic nose based on commercially available sensors used to sniff in the market and identify spoiled/contaminated meat stocked for sale in butcher shops. Using a metal oxide semiconductor-based electronic nose, we measured the smell signature from two of the most common meat foods (beef and fish) stored at room temperature. Food samples were divided into two groups: fresh beef with decayed fish and fresh fish with decayed beef. The prime objective...

  12. Warm fresh whole blood and thoracic traumain iraq and afghanistan.

    Science.gov (United States)

    Keneally, Ryan J; Parsons, Andrew M; Willett, Peter B

    2015-01-01

    Thoracic trauma occurred in 10% of the patients seen at US military treatment facilities in Iraq and Afghanistan and 52% of those patients were transfused. Among those transfused, 281 patients received warm fresh whole blood. A previous report documented improved survival with warm fresh whole blood in patients injured in combat without stratification by injury pattern. A later report described an increase in acute lung injuries after its administration. Survivorship and warm fresh whole blood have never been analyzed in a subpopulation at highest risk for lung injuries, such as patients with thoracic trauma. There may be a heterogeneous relationship between whole blood and survival based on likelihood of a concomitant pulmonary injury. In this report, the relationship between warm fresh whole blood and survivorship was analyzed among patients at highest risk for concomitant pulmonary injuries. Patients with thoracic trauma who received a transfusion were identified in the Joint Theater Trauma Registry. Gross mortality rates were compared between whole blood recipients and patients transfused with component therapy only. The association between each blood component and mortality was determined in a regression model. The overall mortality risk was compared between warm fresh whole blood recipients and non-recipients. Patients transfused with warm fresh whole blood in addition to component therapy had a higher mortality rate than patients transfused only separated blood components (21.3% vs. 12.8%, P warm fresh whole blood in addition to component therapy was not associated with increased mortality risk compared with the transfusion of component therapy only (OR 1.247 [95% CI 0.760-2.048], P = 0.382). Patients with combat related thoracic trauma transfused with warm fresh whole blood were not at increased risk for mortality compared to those who received component therapy alone when controlling for covariates.

  13. Capabilities of diffusion-weighted and fresh blood imaging in depicting fresh thrombus. Presidential award proceedings

    International Nuclear Information System (INIS)

    Ando, Ritsuko; Manabe, Tsutomu; Tazawa, Satoru

    2007-01-01

    We examined the capabilities of diffusion-weighted (DWI) and fresh-blood imaging (FBI) in depicting thrombus. A paper-clay phantom holding test syringes of various sizes filled with either contrast medium or fresh human blood were scanned using a 1.5T magnetic resonance (MR) imaging unit, and apparent diffusion coefficient (ADC) values and signal intensities on DWI and FBI of the specimens were obtained. FBI depicted all the specimens regardless of syringe diameter, but DWI failed to image the syringe measuring 0.5 cm in diameter. B-factors and/or number of acquisitions (NAQ) seemed responsible for DWI's depiction capability. ADC values and signal intensities on DWI and FBI correlated with the viscosity of the contrast medium samples. Clotted blood, the most viscous of the samples, had the smallest ADC value and no relationship with signal intensities on DWI and FBI. Larger b-factors reduced signal intensity in contrast medium on DWI, but signals decreased only minimally in clotted blood. The result suggested that although viscosity was the influential factor for signal intensities on DWI in contrast medium, other factors, such as particle sizes of fibrin and hemoglobin, accounted for the low ADC values in clotting blood. T 2 relaxation time seemed to play a significant role in making signal intensities on DWI irrelevant to b-factors. Despite lapsed time, the clots were persistently hyperintense on FBI with a tendency to decrease only gradually. On DWI, there was a certain period when signal intensities were high and ADC values were low. The signal intensities on DWI and ADC values were considered to be influenced by the process of clot formation, and disappearance of signal seemed likely attributable to degeneration of protein and organization of the clot. (author)

  14. Determination of Properties of Selected Fresh and Processed Medicinal Plants

    Directory of Open Access Journals (Sweden)

    Shirley G. Cabrera

    2015-11-01

    Full Text Available The study aimed to determine the chemical properties, bioactive compounds, antioxidant activity and toxicity level of fresh and processed medicinal plants such as corn (Zea mays silk, pancitpancitan (Peperomiapellucida leaves, pandan (Pandanus amaryllifolius leaves, and commercially available tea. The toxicity level of the samples was measured using the Brine Shrimp Lethality Assay (BSLA. Statistical analysis was done using Statistical Package for Social Sciences (SPSS. Results showed that in terms of chemical properties there is significant difference between fresh and processed corn silk except in crude fiber content was noted. Based on proximate analyses of fresh and processed medicinal plants specifically in terms of % moisture, %crude protein and % total carbohydrates were also observed. In addition, there is also significant difference on bioactive compound contents such as total flavonoids and total phenolics between fresh and processed corn silk except in total vitamin E (TVE content. Pandan and pancit-pancitan showed significant difference in all bioactive compounds except in total antioxidant content (TAC. Fresh pancit-pancitan has the highest total phenolics content (TPC and TAC, while the fresh and processed corn silk has the lowest TAC and TVE content, respectively. Furthermore, results of BSLA for the three medicinal plants and commercially available tea extract showed after 24 hours exposure significant difference in toxicity level was observed. The percentage mortality increased with an increase in exposure time of the three medicinal plants and tea extract. The results of the study can served as baseline data for further processing and commercialization of these medicinal plants.

  15. Identifying risk event in Indonesian fresh meat supply chain

    Science.gov (United States)

    Wahyuni, H. C.; Vanany, I.; Ciptomulyono, U.

    2018-04-01

    The aim of this paper is to identify risk issues in Indonesian fresh meat supply chain from the farm until to the “plate”. The critical points for food safety in physical fresh meat product flow are also identified. The paper employed one case study in the Indonesian fresh meat company by conducting observations and in-depth three stages of interviews. At the first interview, the players, process, and activities in the fresh meat industry were identified. In the second interview, critical points for food safety were recognized. The risk events in each player and process were identified in the last interview. The research will be conducted in three stages, but this article focuses on risk identification process (first stage) only. The second stage is measuring risk and the third stage focuses on determining the value of risk priority. The results showed that there were four players in the fresh meat supply chain: livestock (source), slaughter (make), distributor and retail (deliver). Each player has different activities and identified 16 risk events in the fresh meat supply chain. Some of the strategies that can be used to reduce the occurrence of such risks include improving the ability of laborers on food safety systems, improving cutting equipment and distribution processes

  16. Coupling reverse osmosis with electrodialysis to isolate natural organic matter from fresh waters

    Science.gov (United States)

    This study was undertaken to solve the problem of removal of sulfate and silica from solutions of natural organic matter (NOM) that have been pre-concentrated by reverse osmosis. The goal is the development of a method by which NOM can be concentrated and desalted to obtain a low...

  17. Agrobacterium-Mediated Transformation of Bread and Durum Wheat Using Freshly Isolated Immature Embryos

    Science.gov (United States)

    Wu, Huixia; Doherty, Angela; Jones, Huw D.

    Agrobacterium-mediated transformation of wheat is becoming a viable alternative to the more established biolistic protocols. It offers advantages in terms of simple, low-copy-number integrations and can be applied with similar efficiencies to specific durum wheat and spring and winter bread wheat types varieties.

  18. Prevalence and contamination patterns of Listeria monocytogenes in catfish processing environment and fresh fillets.

    Science.gov (United States)

    Chen, Bang-Yuan; Pyla, Rajkumar; Kim, Tae-Jo; Silva, Juan L; Jung, Yean-Sung

    2010-08-01

    Catfish skins, intestines, fresh fillets, processing surfaces at different production stages, chiller water and non-food contact surfaces were sampled for Listeria monocytogenes and other Listeria species. Among 315 samples, prevalence of L. monocytogenes, Listeria innocua and a group of Listeria seeligeri-Listeria welshimeri-Listeria ivanovii was 21.6, 13.0 and 29.5%, respectively. No Listeria grayi was detected in this survey. While no L. monocytogenes strains were isolated from catfish skins and intestines, the strains were found with a frequency of 76.7% in chilled fresh catfish fillets and 43.3% in unchilled fillets. L. monocytogenes and Listeria spp. were also detected in fish contact surfaces such as deheading machine, trimming board, chiller water, conveyor belts at different stages, and fillet weighing table. Among L. monocytogenes, 1/2b (47.0%), 3b (16.0%) and 4c (14%) were the predominant serotypes isolated, whereas 4b, 4e, 1/2c and 1/2a were detected at much lower frequencies. Genotype analyses of L. monocytogenes isolates using serotyping, pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus (ERIC)-PCR revealed that chiller water represented an important contamination source of L. monocytogenes in the chilled catfish fillets of two processing facilities, whereas fillet weighing table significantly contributed to the catfish fillet contamination of the third facility. This study suggests that L. monocytogenes contamination in the processed catfish fillets originates from the processing environment, rather than directly from catfish. Results from this study can aid the catfish industry to develop a plant-specific proper cleaning and sanitation procedure for equipment and the processing environment designed to specifically target L. monocytogenes contamination. Copyright 2010 Elsevier Ltd. All rights reserved.

  19. Occurrence of potentially toxigenic mould species in fresh salads of different kinds of ready-for-use vegetables

    Directory of Open Access Journals (Sweden)

    Kocić-Tanackov Sunčica D.

    2010-01-01

    Full Text Available In the mycological survey of fresh salads of different vegetables, the collected samples were tested for total counts of moulds with special attention paid to the presence of potentially toxigenic species. The survey also included the isolation and the identification of species, as well as the evaluation of mycotoxin biosynthesis ability of potential producers of ochratoxin A (OA and sterigmatocystin (STC. Mould counts ranged from 10.0 to 4.7x102 cfu g-1. The most common moulds found in fresh salads were Cladosporium (42.89%, Penicillium (25.78%, Aspergillus (14.67% and Alternaria (6.89%. C. cladosporioides (40.44%, followed by A. niger (10.22%, P. aurantiogriseum (7.55%, A. alternata (6.89% and Fusarium spp. (3.11% were the most dominating species. Other species were represented with 2.22% (Eurotium spp., 1.56% (Botrytis spp., 0.67% (Phoma spp., 0.44% (Geotrichum spp., Mucor spp., Phialophora spp. and 0.22% (Emericella spp., Paecilomyces spp., Trichoderma spp., Xeromyces spp.. Twenty-two of 41 identified mould species were potentially toxigenic, which accounted for 46.18% of the total isolated population. The most frequent were the potential producers of ochratoxin A (17.77%. Potential producers of moniliformin were isolated in 3.11% of samples, while producers of fumonisin and STC were found in 2.67% and 2.44% of samples, respectively. The tested isolates of OA producers did not demonstrate the ability to biosynthetise this mycotoxins, but two out of five isolates of A. versicolor were found to biosynthesise STC in doses of 109.2 ng mL-1 and 56.3 ng mL-1. The obtained results indicate that such products may threaten human health, considering that isolated species were potentially toxigenic, while isolates of A. versicolor also biosynthesised STS.

  20. Isolation World

    OpenAIRE

    Núñez Martín, Eugeni

    2012-01-01

    El trabajo de fin de grado tiene como nombre “Isolation World”, que en su traducción literal significa “Aislamiento del mundo”, un videojuego diseñado y creado desde cero en su totalidad, utilizando herramientas y conocimiento de lógica en programación que se han ido aprendiendo y desarrollando a lo largo de la carrera.

  1. Isolation and Identification of Fungi Associated with the Spoilage of ...

    African Journals Online (AJOL)

    This study was carried out in Sokoto Metropolis to isolate and identify fungi associated with the deterioration of sweet orange fruits. A total of one hundred samples of fresh sweet Oranges (Citrus sinensis L) were used. First, a total of seventy samples were obtained from the three selected marketing centres in Sokoto ...

  2. Antifungal Capacity of Lactic Acid Bacteria Isolated From Salad ...

    African Journals Online (AJOL)

    This study explores the use of lactic acid bacteria from fresh salad vegetables to inhibit fungal growth. The antifungal assay was done using the agar well diffusion method as reported by Schillinger and Lucke (1989). The largest zone of inhibition (25mm) was recorded by the antagonistic activity of the isolate identified to ...

  3. Toward an Improved Understanding of the Global Fresh Water Budget

    Science.gov (United States)

    Hildebrand, Peter H.

    2005-01-01

    The major components of the global fresh water cycle include the evaporation from the land and ocean surfaces, precipitation onto the Ocean and land surfaces, the net atmospheric transport of water from oceanic areas over land, and the return flow of water from the land back into the ocean. The additional components of oceanic water transport are few, principally, the mixing of fresh water through the oceanic boundary layer, transport by ocean currents, and sea ice processes. On land the situation is considerably more complex, and includes the deposition of rain and snow on land; water flow in runoff; infiltration of water into the soil and groundwater; storage of water in soil, lakes and streams, and groundwater; polar and glacial ice; and use of water in vegetation and human activities. Knowledge of the key terms in the fresh water flux budget is poor. Some components of the budget, e.g. precipitation, runoff, storage, are measured with variable accuracy across the globe. We are just now obtaining precise measurements of the major components of global fresh water storage in global ice and ground water. The easily accessible fresh water sources in rivers, lakes and snow runoff are only adequately measured in the more affluent portions of the world. presents proposals are suggesting methods of making global measurements of these quantities from space. At the same time, knowledge of the global fresh water resources under the effects of climate change is of increasing importance and the human population grows. This paper provides an overview of the state of knowledge of the global fresh water budget, evaluating the accuracy of various global water budget measuring and modeling techniques. We review the measurement capabilities of satellite instruments as compared with field validation studies and modeling approaches. Based on these analyses, and on the goal of improved knowledge of the global fresh water budget under the effects of climate change, we suggest

  4. Option contracts in fresh produce supply chain with circulation loss

    Directory of Open Access Journals (Sweden)

    Chong Wang

    2013-03-01

    Full Text Available Purpose: The purpose of this paper is to investigate management decisions via option contracts in a two-stage supply chain in which a fresh produce supplier sells to a retailer, considering the circulation loss of the fresh produce. Design/methodology/approach: Authors propose a Stackelberg model to analyze the supply chain members’ decisions in the decentralized supply chain compared with the integrated one under the newsvendor framework. Findings: The results illustrate that there exists a unique optimal option order quantity for the retailer and a unique optimal option order price for the supplier giving certain conditions; furthermore, option contracts cannot coordinate the fresh produce supply chain when the retailer only orders options. Originality/value: Agricultural products especially fresh produce’s characteristics such as circulation loss and high risk are considered. Option contracts and game theory are combined to manage the fresh produce supply chain’s risk. The proposed tool and models are hoped to shed light to the future works in the field of supply chain risk management.

  5. Planning and control in fresh food supply chains

    DEFF Research Database (Denmark)

    Damgaard, Cecilie Maria; Chabada, Lukas; Dukovska-Popovska, Iskra

    2013-01-01

    directions. Design/methodology/approach The paper is based on a structured literature review of articles with the main focus on P&C of fresh food products. The review is based on a range of published works from main journals on supply chain management over the last 10 years. The gaps and challenges...... directions in this area. Practical implications The paper enhances the focus on P&C in FFSC in practice, and defines important implications for why and how P&C should be practiced from a supply chain perspective. Original/value The paper presents an overview of the literature on P&C issues of fresh food......Purpose The purpose of this paper is to determine the current state of research in the planning and control (P&C) literature in regards to fresh food supply chains (FFSC). Based on the literature review, important research areas are identified and serve as guidelines for defining future research...

  6. Effect of dim light irradiation on preservation of fresh lettuce

    International Nuclear Information System (INIS)

    Harada, F.; Uchino, T.; Akimoto, K.; Hu, W.

    2001-01-01

    In order to preserve fresh lettuce, the dim light irradiation storage was investigated. Lettuce (Lactuca sativa L.) stored for about 120 hours at 5 or 20 deg C under continuous illumination at 0 (darkness), 1.6, 3.4, 6.5, 13 or 19.7 micro mol/m**-2/s**-1 photosynthetic photon flux. The light compensation point was about 3.4 micro mol/m**-2/s**-1 at 5 deg C, about 19.7 micro mol/m**-2/s**-1 at 20 deg C. Fresh weight of lettuce decreased by promotion of transpiration caused by the dim light. Accordingly the root of lettuce should not be removed, so as to up-take water. The optimum dim light irradiation preserved the chlorophyll content in lettuce leaf or increased it. Therefore it appeared that the dim light irradiation was effective for the fresh lettuce preservation

  7. Uptake of {sup 137}Cs by fresh water fish

    Energy Technology Data Exchange (ETDEWEB)

    Man, C.K.; Kwok, Y.H

    2000-02-01

    The uptake and discharge rates of {sup 137}Cs by fresh water fish at different radionuclide concentrations have been studied. A dual compartment model was used to fit the experimental data. The discharge rates have been found to be negligible for the duration of the experiment of 10 days. The uptake rates were independent of radionuclide concentrations for a particular type of fresh water fish and were different for different types of fish. The uptake rates of carp, tilapia and snakehead were 1.58, 1.66 and 2.23, in unit of 10{sup -6} h{sup -1}, respectively. It was also estimated that the consumption of fresh water fish, even if the water were contaminated as much as that in the Chernobyl accident, leads to negligible latent cancer fatality to the Hong Kong population.

  8. Revascularisation of fresh compared with demineralised bone grafts in rats

    DEFF Research Database (Denmark)

    Solheim, E; Pinholt, E M; Talsnes, O

    2001-01-01

    Revascularisation of bone grafts is influenced by both the anatomical origin and the pre-implantation processing of the graft. We investigated the revascularisation by entrapment of 141Ce (cerium)-labelled microspheres in large, fresh and demineralised syngeneic grafts of predominantly cancellous...... (iliac bone) or cortical (tibial diaphysis) bone three weeks after heterotopic implantation in rats. The mean (SD) 141Ce deposition index (counts per minute (cpm) of mg recovered implant/cpm of mg host iliac bone) was higher in fresh iliac bone grafts, 0.98 (0.46) compared to that of demineralised iliac...... bone, 0.32 (0.20), p bone grafts, 0.51 (0.27), p = 0.007. We found no significant difference in the mean 141Ce deposition index between fresh tibial bone grafts and demineralised tibial bone grafts, 0.35 (0.42), p = 0.4, or between demineralised tibial grafts and demineralised...

  9. Differentiation of Staphylococcus aureus from freshly slaughtered poultry and strains 'endemic' to processing plants by biochemical and physiological tests.

    Science.gov (United States)

    Mead, G C; Norris, A P; Bratchell, N

    1989-02-01

    A comparison was made of 27 'endemic' strains of Staphylococcus aureus and 35 strains from freshly slaughtered birds, isolated at five commercial slaughterhouses processing chickens or turkeys. Of 112 biochemical and physiological tests used, 74 gave results which differed among the strains. Cluster analysis revealed several distinct groupings which were influenced by strain type, processing plant and bird origin; these included a single group at the 72% level of similarity containing most of the 'endemic' strains. In comparison with strains from freshly slaughtered birds, a higher proportion of 'endemic' strains produced fibrinolysin, alpha-glucosidase and urease and were beta-haemolytic on sheep-blood agar. The 'endemic' type also showed a greater tendency to coagulate human but not bovine plasma, and to produce mucoid growth and clumping. The last two properties, relevant to colonization of processing equipment, were less evident in heart infusion broth than in richer media or process water collected during defeathering of the birds.

  10. Bioremediation of Zn, Cu, Mg and Pb in Fresh Domestic Sewage by Brevibacterium sp

    International Nuclear Information System (INIS)

    Ojoawo, S. O.; Rao, C. V.; Goveas, L. C.

    2016-01-01

    The study applied an isolated Brevibacterium sp. (MTCC 10313) for bioremediation of Zn, Cu, Mg and Pb in domestic sewage. Batch culture experiments were performed on both the fresh and stale sewage samples with glucose supplementation of 1-8g/l. Nutrient broth medium was prepared, sterilized and p H adjusted to 6.5-6.8. 1% of the Brevibacteria sp. stock was inoculated into the broth and maintained at 370C for 24 hours in shaker incubator at 120 rpm. Another 1% of fresh grown sub-culture of broth was inoculated into supplemented and sterilized samples. Optical Density was taken at 600nm, growth monitored over 12 days, cultured samples denatured with TCA and centrifuged, supernatants filtered and analyzed with AAS, Settled pellets oven dried, subjected to SEM analysis for morphology and constituents determination. Fresh sewage samples permitted bacterial growth and facilitated bioremediation of Zn, Cu and Mg through metal uptake and bioabsoption by Brevibacteria sp. This effectively reduced concentration of heavy metals, with treatment efficiency order Cu>Zn>Mg, and respective removal percentages of 77, 63 and 55. The optimum glucose concentration for effective bioremediation found as 2g/l for Zn and Cu, and 8g/l for Mg. Pb was resistant to bioremediation with Brevibacteria sp. Stale sewage produced inhibitory substances preventing adequate growth of bacterium with no bioremediation. Bioremediation with Brevibacteria sp. is found effective in removal of micro-units of Zn, Cu and Mg from domestic sewage. As a readily available low-cost agent, it is recommended for large- scale application on those metals while Pb should be further subjected to advanced treatments.

  11. Fertilization and Embryo Development of Fresh and Cryopreserved Sibling Oocytes

    Directory of Open Access Journals (Sweden)

    Robert F. Casper

    2010-01-01

    Full Text Available Background: Oocyte cryopreservation is potentially the best way to preserve female fertility forunmarried women or young girls at risk of losing ovarian function. The aim of this study was tocompare fertilization and embryo development in frozen-thawed oocytes to their fresh siblings inwomen undergoing in vitro fertilization (IVF and embryo transfer (ET.Materials and Methods: Eleven infertile women undergoing infertility treatment, between theages of 24 to 37 years (mean ± SD = 31.6 ± 3.5, were included in this study. Mature oocytesfrom each patient were randomized into cryopreserved and fresh groups prior to intracytoplasmicsperm injection (ICSI. One hundred and thirty nine oocytes were retrieved, of which 105 were atmetaphase II (MII. Forty- five fresh MII oocytes were kept in culture whereas their sibling 60 MIIoocytes were cryopreserved using a slow cooling protocol. The frozen oocytes remained in LN2for 2 hours before thawing. ICSI was performed 1-2 hours after thawing for frozen oocytes and 4-5hours after retrieval for fresh oocytes. Fertilization and embryo development were compared.Results: Following thawing, 31 oocytes (51.6 % survived and 22 fertilized (79% while 32 freshoocytes fertilized upon ICSI (71%. The mean ± SE scores for embryos developing from frozenthawedoocytes were significantly lower at 48 and 72 hours post-ICSI than for embryos resultingfrom fresh oocytes (p<0.05.Conclusion: Our data demonstrated that oocyte freezing resulted in acceptable survival ratesfollowing cryopreservation, and similar fertilization rates following ICSI as compared to the freshsibling oocytes. However the number of blastomeres and the embryo quality on day three wassuperior in embryos from fresh oocytes when compared to the frozen oocytes.

  12. Does generic advertising work? A systematic evaluation of the Danish campaign for fresh fish

    DEFF Research Database (Denmark)

    Scholderer, Joachim; Grunert, Klaus G.

    2001-01-01

    Before and after the 3-year generic advertising campaign for fresh fish in Denmark, representative consumer samples were surveyed with regard to their attitudes towards fresh fish, perceived family norms, availability of fresh fish in shops, meal preparation skills, intentions to buy fresh fish...

  13. Does generic advertising work? A systematic evaluation of the Danish campaign for fresh fish

    DEFF Research Database (Denmark)

    Scholderer, Joachim; Grunert, Klaus G.

    Before and after the 3-year generic advertising campaign for fresh fish in Denmark, representative consumer samples were surveyed with regard to their attitudes towards fresh fish, perceived family norms, availability of fresh fish in shops, meal preparation skills, intentions to buy fresh fish...

  14. Reduction of radiation injury of fresh agricultural products by saccharide

    International Nuclear Information System (INIS)

    Hayashi, Toru; Todoroki, Setsuko

    1998-01-01

    To establish irradiation technologies as one of alternative technology of methyl bromide fumigation, radiation sensitivities for each kind of fresh agricultural products and reduction of radiation injury were investigated. Fresh vegetables and flowers such as cabbage, sprouts, asparagus, lettuce, chrysanthemum, carnation, rose, etc. were used and irradiated with 750 Gy γ-ray. Flowers received radiation injury were soaked into various kinds of solutions for one night, then they were irradiated with 500 Gy γ-ray. They showed different radiation sensitivities. Cruciferae plant showed radioresistance and Compositae plant radiosensitivity. A keeping quality agent for cut flowers indicated protection effect on radiation injury. (S.Y.)

  15. Fresh chicken as main risk factor for campylobacteriosis, Denmark

    DEFF Research Database (Denmark)

    Wingstrand, Anne; Neimann, Jakob; Engberg, Jørgen

    2006-01-01

    We report the findings of a case-control study of risk factors for sporadic cases of human campylobacteriosis in Denmark. In 3 different analytical models, the main domestic risk factor identified was eating fresh, unfrozen chicken. Specifically, 28 of 74 domestically acquired case-patients were...... exposed to fresh chicken compared with 21 of 114 controls (multivariate matched odds ratio 5.8; 95% confidence interval 2.1-15.9). In contrast, a risk from eating other poultry, including previously frozen chicken, was only indicated from borderline significant 2-factor interactions. The marked increase...

  16. Bionic Duplication of Fresh Navodon septentrionalis Fish Surface Structures

    Directory of Open Access Journals (Sweden)

    Bing Qu

    2011-01-01

    Full Text Available Biomimetic superhydrophobic surface was fabricated by replicating topography of the fresh fish skin surface of Navodon septentrionalis with polydimethylsiloxane (PDMS elastomer. A two-step replicating method was developed to make the surface structure of the fresh fish skin be replicated with high fidelity. After duplication, it was found that the static contact angle of the replica was as large as 173°. Theoretic analysis based on Young's and Cassie-Baxter (C-B model was performed to explain the relationship between structure and hydrophobicity.

  17. Synergies between renewable energy and fresh water production. Scoping study

    Energy Technology Data Exchange (ETDEWEB)

    Geurts, F.; Noothout, P.; Schaap, A. [Ecofys Netherlands, Utrecht (Netherlands)

    2011-02-15

    The IEA Implementing Agreement for Renewable Energy Technology Deployment (IEA-RETD) investigated the opportunities for coupling renewable energy systems with fresh water supply systems. The four main conclusions of the scoping study, carried out by Ecofys, are: (1) Fresh water production based on desalination technologies provide most options for synergies with renewable energy production; (2) Linking desalination to renewable sources is currently not economically viable; (3) There is a large potential for small scale (decentralised) desalination plants; (4) Current commercially-sized desalination technologies are in need of a constant operation point. Reverse osmosis and thermal membrane technologies might give future synergies as deferrable load.

  18. Fresh Chicken as Main Risk Factor for Campylobacteriosis, Denmark

    OpenAIRE

    Wingstrand, Anne; Neimann, Jakob; Engberg, Jørgen; Nielsen, Eva Møller; Gerner-Smidt, Peter; Wegener, Henrik C.; Mølbak, Kåre

    2006-01-01

    We report the findings of a case-control study of risk factors for sporadic cases of human campylobacteriosis in Denmark. In 3 different analytical models, the main domestic risk factor identified was eating fresh, unfrozen chicken. Specifically, 28 of 74 domestically acquired case-patients were exposed to fresh chicken compared with 21 of 114 controls (multivariate matched odds ratio 5.8; 95% confidence interval 2.1–15.9). In contrast, a risk from eating other poultry, including previously f...

  19. Reduction of radiation injury of fresh agricultural products by saccharide

    Energy Technology Data Exchange (ETDEWEB)

    Hayashi, Toru; Todoroki, Setsuko [National Food Research Inst., Tsukuba, Ibaraki (Japan)

    1998-02-01

    To establish irradiation technologies as one of alternative technology of methyl bromide fumigation, radiation sensitivities for each kind of fresh agricultural products and reduction of radiation injury were investigated. Fresh vegetables and flowers such as cabbage, sprouts, asparagus, lettuce, chrysanthemum, carnation, rose, etc. were used and irradiated with 750 Gy {gamma}-ray. Flowers received radiation injury were soaked into various kinds of solutions for one night, then they were irradiated with 500 Gy {gamma}-ray. They showed different radiation sensitivities. Cruciferae plant showed radioresistance and Compositae plant radiosensitivity. A keeping quality agent for cut flowers indicated protection effect on radiation injury. (S.Y.)

  20. Evidence for a role of biosurfactants produced by Pseudomonas fluorescens in the spoilage of fresh aerobically stored chicken meat.

    Science.gov (United States)

    Mellor, Glen E; Bentley, Jessica A; Dykes, Gary A

    2011-08-01

    Fresh chicken meat is a fat-rich environment and we therefore hypothesised that production of biosurfactants to increase bioavailability of fats may represent one way in which spoilage bacteria might enhance the availability of nutrients. Numbers of Pseudomonas were determined on a total of 20 fresh and 20 spoiled chicken thighs with skin. A total of 400 randomly isolated Pseudomonas colonies from fresh (200) and spoiled (200) chicken were screened for the presence of biosurfactant production. Biosurfactant producing strains represented 5% and 72% of the Pseudomonas spp. isolates from fresh (mean count 2.3 log(10) cfu g(-1)) and spoiled (mean count 7.4 log(10) cfu g(-1)) chicken skin, respectively. Partially-purified biosurfactants derived from a subgroup of four Pseudomonasfluorescens strains obtained through the screening process were subsequently used to investigate the role that the addition of these compounds plays in the spoilage of aerobically stored chicken. Emulsification potential of the four selected biosurfactants was measured against a range of hydrocarbons and oils. All four biosurfactants displayed a greater ability to emulsify rendered chicken fat than hydrocarbons (paraffin liquid, toluene and hexane) and oils (canola, olive, sunflower and vegetable). Storage trials (4 °C) of chicken meat treated with the four selected biosurfactants revealed a significantly greater (P increase in total aerobic count (1.3-1.7 log(10) cfu g(-1)) occurred following one day of incubation. These results indicate that biosurfactants produced by Pseudomonas spp. may play an important role in the spoilage of aerobically stored chicken meat by making nutrients more freely available and providing strains producing them with a competitive advantage. Copyright © 2011 Elsevier Ltd. All rights reserved.

  1. Subcritical Noise Analysis Measurements with Fresh and Spent Research Reactor Fuels Elements

    International Nuclear Information System (INIS)

    Valentine, T.E.; Mihalczo, J.T.; Kryter, R.C.; Miller, V.C.

    1999-01-01

    The verification of the subcriticality is of utmost importance for the safe transportation and storage of nuclear reactor fuels. Transportation containers and storage facilities are designed such that nuclear fuels remain in a subcritical state. Such designs often involve excess conservatism because of the lack of relevant experimental data to verify the accuracy of Monte Carlo codes used in nuclear criticality safety analyses. A joint experimental research program between Oak Ridge National Laboratory, Westinghouse Safety Management Solutions, Inc., and the University of Missouri was initiated to obtain measured quantities that could be directly related to the subcriticality of simple arrays of Missouri University Research Reactor (MURR) fuel elements. A series of measurement were performed to assess the reactivity of materials such as BORAL, stainless steel, aluminum, and lead that are typically used in the construction of shipping casks. These materials were positioned between the fuel elements. In addition, a limited number of measurements were performed with configurations of fresh and spent (irradiated) fuel elements to ascertain the reactivity of the spent fuel elements. In these experiments, fresh fuel elements were replaced by spent fuel elements such that the subcritical reactivity change could be measured. The results of these measurements were used by Westinghouse Safety Management Solutions to determine the subcriticality of MURR fuel elements isolated by absorbing materials. The measurements were interpreted using the MCNP-DSP Monte Carlo code to obtain the subcritical neutron multiplication factor k(sub eff), and the bias in K(sub eff) that are used in criticality safety analyses

  2. Chemical Constituents and Antioxidant Activity of fresh leaves of psidium guajava cultivated in pakistan

    International Nuclear Information System (INIS)

    Begum, S.; Ali, S.N.; Tauseef, S.

    2014-01-01

    The in vitro antioxidant activities of the methanol extract of fresh leaves of Psidium guajava cultivated in Pakistan and its different fractions were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. The methanol extract, main ethyl acetate fraction and its polar sub fraction showed high free radical scavenging activity with EC50 11.72, 11.72 and 46.8 micro g/mL respectively. The first two values are comparable with that of reference compound ascorbic acid (EC50 9.4 ?g/mL). The known antioxidants gallic acid (1), methyl ferulate (2) and methyl p-E-coumarate (3) were isolated from the ethyl acetate insoluble fraction. Their structures were identified by mass, 1H- and 13C-NMR spectroscopy. Compounds 2 and 3 are reported for the first time from the genus Psidium. (author)

  3. Emerging Perspectives on the Natural Microbiome of Fresh Produce Vegetables

    Directory of Open Access Journals (Sweden)

    Colin R. Jackson

    2015-04-01

    Full Text Available Plants harbor a diverse microbiome existing as bacterial populations on the leaf surface (the phyllosphere and within plant tissues (endophytes. The composition of this microbiome has been largely unexplored in fresh produce vegetables, where studies have tended to focus on pathogen detection and survival. However, the application of next-generation 16S rRNA gene sequencing approaches is beginning to reveal the diversity of this produce-associated bacterial community. In this article we review what is known about the composition of the microbiome of fresh produce vegetables, placing it in the context of general phyllosphere research. We also demonstrate how next-generation sequencing can be used to assess the bacterial assemblages present on fresh produce, using fresh herbs as an example. That data shows how the use of such culture-independent approaches can detect groups of taxa (anaerobes, psychrophiles that may be missed by traditional culture-based techniques. Other issues discussed include questions as to whether to determine the microbiome during plant growth or at point of purchase or consumption, and the potential role of the natural bacterial community in mitigating pathogen survival.

  4. Effect of solarization with fresh chicken manure on verticillium wilt ...

    African Journals Online (AJOL)

    The present study was carried out to evaluate the effect of reducing wilt disease through the medium of fresh chicken manure (FCM) mixed with soil before solarized and then artificial Verticillium dahliae (V.d) inoculation on yield of eggplant (Solanum melongena L.) under field conditions. According to the splitplot design, ...

  5. Relating the Electrical Resistance of Fresh Concrete to Mixture Proportions.

    Science.gov (United States)

    Obla, K; Hong, R; Sherman, S; Bentz, D P; Jones, S Z

    2018-01-01

    Characterization of fresh concrete is critical for assuring the quality of our nation's constructed infrastructure. While fresh concrete arriving at a job site in a ready-mixed concrete truck is typically characterized by measuring temperature, slump, unit weight, and air content, here the measurement of the electrical resistance of a freshly cast cylinder of concrete is investigated as a means of assessing mixture proportions, specifically cement and water contents. Both cement and water contents influence the measured electrical resistance of a sample of fresh concrete: the cement by producing ions (chiefly K + , Na + , and OH - ) that are the main source of electrical conduction; and the water by providing the main conductive pathways through which the current travels. Relating the measured electrical resistance to attributes of the mixture proportions, such as water-cement ratio by mass ( w/c ), is explored for a set of eleven different concrete mixtures prepared in the laboratory. In these mixtures, w/c , paste content, air content, fly ash content, high range water reducer dosage, and cement alkali content are all varied. Additionally, concrete electrical resistance data is supplemented by measuring the resistivity of its component pore solution obtained from 5 laboratory-prepared cement pastes with the same proportions as their corresponding concrete mixtures. Only measuring the concrete electrical resistance can provide a prediction of the mixture's paste content or the product w*c ; conversely, when pore solution resistivity is also available, w/c and water content of the concrete mixture can be reasonably assessed.

  6. Fresh or dehydrated spineless cactus in diets for lambs

    Directory of Open Access Journals (Sweden)

    Solano Felipe Just de Andrade

    2016-05-01

    Full Text Available The objective was to evaluate the effect of partial or total replacement of Tifton 85 hay with fresh or dehydrated spineless cactus on performance and body weight components of sheep. Thirty hair lambs, with 19 kg ± 0.35 kg initial weight, were randomly divided into five treatments, namely: Tifton 85 Hay (HA; Tifton 85 Hay + fresh spineless cactus (HAFC; Tifton 85 Hay + dehydrated spineless cactus (HADC; fresh spineless cactus (FC; dehydrated spineless cactus (DC. In addition to the spineless cactus and hay, the complete mixture contained soybean meal, mineral salt and limestone in all treatments. Animals were confined for 75 days and then slaughtered. The weight of body components was recorded. Dry matter intake, total digestible nutrients intake and voluntary water intake were higher (p 0.05 by treatments. The cold carcass weight, rib eye area, weight and yield of the skin, liver, kidney and large intestine were higher (p < 0.05 for the DC and HADC treatments. Partial or total replacement of Tifton 85 hay with fresh or dehydrated spineless cactus, in complete diets for sheep, increases the weight of the cold carcass and non-carcass components.

  7. Challenges with mechanical harvesting of blueberries for the fresh market

    Science.gov (United States)

    Southern highbush blueberries (SHB) (Vaccinium corymbosum L.) are mostly hand harvested for the fresh market. Hand harvesting of blueberry is labor intensive and costly. Efforts are under way to develop blueberry genotypes that will develop less bruising after impact with hard surfaces on the harv...

  8. Microbial and Sensory Quality of Freshly Processed and ...

    African Journals Online (AJOL)

    The samples were also evaluated for difference and preference. The study showed that the reconstituted beverage had better microbiological quality with detectable difference between the two samples with the fresh sample being preferred. Key words: Millet grains, Kununzaki, microbial quality, sensory quality. J Food Tech ...

  9. Effect of accessions on the chemical quality of fresh pumpkin ...

    African Journals Online (AJOL)

    Twenty (20) fresh pumpkin fruits were assessed for dry matter, total soluble solid, total sugar, reducing sugar, titratable acidity, pH, ascorbic acid and sugar-to-acid ratio. Statistically significant (P < 0.01) differences were found for dry matter, total soluble solid, total sugar, reducing sugar, titratable acidity, ascorbic acid, pH ...

  10. Biodegradability of unused lubricating brake fluids in fresh and ...

    African Journals Online (AJOL)

    The biodegradability of four unused lubricating brake fluids (Total brake fluid, Allied brake fluid, Oando brake fluid and Ate brake fluid) was carried out in fresh and marine water obtained from Isiokpo stream and Bonny river of the Niger Delta, South South Nigeria. Biodegradability, of the brake fluids were obtained after a 56 ...

  11. Shipment of VINCA Institute's HEU fresh fuel to Russia

    International Nuclear Information System (INIS)

    Pesic, Milan; Sotic, Obrad

    2002-01-01

    This paper shows, for the first time, the basic data related to the recent shipment of the fresh HEU fuel elements from Yugoslavia back to Russia for uranium down blending. In this way, Yugoslavia gives its contribution to the RERTR program and to the world's joint efforts to prevent possible terrorist action against nuclear material potentially usable for production of nuclear weapons. (author)

  12. Assessment of the contamination of fresh water ecosystems by ...

    African Journals Online (AJOL)

    This paper assessed reports and records of the contamination of fresh water ecosystem by pesticides and herbicides in irrigated rice fields and their effects on fish production. It highlights pesticides and herbicides application in agriculture in view of the transfer and degradation processes. The pesticides often remain ...

  13. Determination of long-term effects of consecutive effective fresh ...

    African Journals Online (AJOL)

    The aim of the study was to determine the weed density and the most economical way of weed control in eggplant (Solanum melongena) fields contaminated with Verticillium dahliae (Kleb) after the application of fresh chicken manure and solarization in the second year as the same crop was grown. The effect of solarization ...

  14. Post-harvest decay in fresh-cut lettuce

    Science.gov (United States)

    Shelf-life of fresh-cut lettuce was assessed in two mapping population (Salinas 88 x La Brillante, and Pavane x Parade. The most significant QTL was detected in both populations on linkage group 4. This QTL was detected in seven experiments grown in different environments. Molecular markers are bein...

  15. HACCP implementation in the production of fresh-cut produce

    Science.gov (United States)

    The number of foodborne illness outbreaks linked to fresh produce has increased in the last few years. Shiga-toxigenic Escherichia coli and Salmonella have been implicated as major bacterial pathogens of concern to produce safety. Microbial contamination of produce may occur anytime during the prod...

  16. Fresh heat is in demand; Frische Waerme ist beliebt

    Energy Technology Data Exchange (ETDEWEB)

    Augsten, Eva

    2012-10-22

    Increasingly, solar heat customers are getting their hot water fresh from the flow-through station, even in single-family dwellings. Stations are getting more compact, more intelligent, and also more efficient. So-called legionella filters should be used with caution.

  17. Shear rheological properties of fresh human faeces with different ...

    African Journals Online (AJOL)

    2014-03-11

    Mar 11, 2014 ... Short communication. Shear rheological properties of fresh human faeces with different moisture content. SM Woolley1, RS Cottingham1, J Pocock1 and CA Buckley1*. 1Pollution Research Group, School of Engineering, University of KwaZulu-Natal, King George V Avenue, Berea 4041, South Africa.

  18. Uptake of tritiated lysine by fresh water alga, Scenedesmus obliquus

    International Nuclear Information System (INIS)

    Gogate, S.S.; Krishnamoorthy, T.M.

    1983-01-01

    Tritium uptake by fresh water alga. S.obliquus was studied using tritium labelled lysine, and a sequential solvent extraction procedure was used to study the distribution of tritium in different organic constituents of the algal cells. The accumulation of tritium in the algal cells was found to be 3-4 orders of magnitude more than that obtained for tritiated water. (author)

  19. Incidence of chilling injury in fresh-cut 'Kent' mangoes

    Science.gov (United States)

    The preferred storage temperature for fresh-cut fruits in terms of visual quality retention is around 5 °C, which is considered to be a chilling temperature for chilling sensitive tropical fruits like mango (Mangifera indica L.). Changes in visual and compositional quality factors, aroma volatile pr...

  20. Fresh fish ( Clarias gariepinus ) marketing system in major towns of ...

    African Journals Online (AJOL)

    This study was carried out to investigate marketing system, market channel, nature of costs, market margins and the factors determining the marketing profit of fresh fish (Clarias gariepinus) in major towns in Ibarapa Zone, Oyo state. Data were collected through the use of questionnaire administered to the fish traders.

  1. Internet Usage In The Fresh Produce Supply Chainin China

    Science.gov (United States)

    Xu, Xiaoxiao; Duan, Yanqing; Fu, Zetian; Liu, Xue

    Although effective implementation of the Internet technologies has a great potential for improving efficiency and reducing wastage within the fresh produce supply chain. the situation of the Internet usage by SMEs (small and medium sized enterprises) in the fresh produce supply chain is still unclear in China. As the main players, SMEs haven't been given enough attention from both academics and governments. Therefore, this research attempts to address this issue by, first, investigating the current usage of the Internet and related software by Chinese SMEs in the fresh produce supply chain, and then, by identifying enablers and barriers faced by SMEs to call government's attention. As a part of an EU-Asia IT&C funded project, a survey was carried out with SMEs in this industry from five major cities in China. The results reveal that in the relatively developed areas of China, SMEs in the fresh produce supply chain are rapidly adopting the Internet and software packages, but the level of adoption varies greatly and there is a significant lack of integration among the supply chain partners. Chinese SMEs are keen to embrace emerging technologies and have acted to adopt new software and tools. Given that cost of implementation is not a barrier, their concern over legal protection and online security must be addressed for further development.

  2. Laboratory evaluation of freshly prepared juice from garlic ( Allium ...

    African Journals Online (AJOL)

    Statistically significant (P<0.05) reduced grain loss was also observed in all the treatments when compared with the control. The juice prepared from an indigenous Nigerian garlic cultivar (GUN) was more lethal (causing 93% adult mortality), when applied topically on the freshly emerged S. zeamais adults, compared to the ...

  3. Availability of Fresh Produce in Nutrition Assistance Programs.

    Science.gov (United States)

    Food and Nutrition Service (USDA), Alexandria, VA. Office of Analysis and Evaluation.

    Noting that fruit and vegetable consumption is an important component of a balanced diet consistent with the "Dietary Guidelines for Americans" and the Food Guide Pyramid, this report fulfills a request from the Appropriations Committee Directives to analyze current levels of fresh produce in Department of Agriculture (USDA) nutritional…

  4. Osmotic Power: A Fresh Look at an Old Experiment

    Science.gov (United States)

    Dugdale, Pam

    2014-01-01

    Electricity from osmotic pressure might seem a far-fetched idea but this article describes a prototype in Norway where the osmotic pressure generated between salt and fresh water drives a turbine. This idea was applied in a student investigation, where they were tasked with researching which alternative materials could be used for the…

  5. Simulation of heavy metal contamination of fresh water bodies: toxic ...

    African Journals Online (AJOL)

    Michael Horsfall

    www.bioline.org.br/ja. Simulation of heavy metal contamination of fresh water bodies: toxic effects in the ... 96 hours (though sampling was done at the 48th hour). Biochemical markers of ... silver, while enhancing the bioavailability of mercury in Ceriodaphnia ..... Biochemical and molecular disorders of bilirubin metabolism.

  6. Fresh frozen cadaver workshops for advanced vascular surgical training.

    Science.gov (United States)

    Jansen, Shirley; Cowie, Margaret; Linehan, John; Hamdorf, Jeffery M

    2014-11-01

    Reduction in working hours, streamlined training schemes and increasing use of endovascular techniques has meant a reduction in operative experience for newer vascular surgical trainees, especially those exposures which are not routinely performed such as thoracoabdominal, thoracotomy and retroperitoneal aortic, for example. This paper describes an Advanced Anatomy of Exposure course which was designed and convened at the Clinical Training & Evaluation Centre in Western Australia and uses fresh frozen cadavers. Feedback was obtained from the participants who attended over three courses by questionnaire. Feedback was strongly positive for the course meeting both its learning outcomes and personal learning objectives, and in addition, making a significant contribution to specialty skills. Most participants thought the fresh frozen cadaveric model significantly improved the learning objectives for training. The fresh frozen cadaver is an excellent teaching model highly representative of the living open surgical scenario where advanced trainees and newly qualified consultants can improve their operative confidence and consequently patient safety in vascular surgery. An efficient fresh frozen cadaver teaching programme can benefit many health professionals simultaneously maximizing the use of donated human tissue. © 2013 Royal Australasian College of Surgeons.

  7. Effect of salinity stress on plant fresh weight and nutrient ...

    African Journals Online (AJOL)

    Effect of salinity stress on plant fresh weight and nutrient composition of some Canola ( Brassica napus L.) cultivars. ... K+, Ca2+ and K+/Na+ contents in plants decreased by salt stress, but Na+ and Cl- content in the roots, ... from 32 Countries:.

  8. Strontium-90 and cesium-137 in fresh water

    International Nuclear Information System (INIS)

    1978-01-01

    Japan Chemical Analysis Center has analysed the strontium-90 and Cesium-137 contents in fresh water from 7 prefectures in Japan by the commission of Science and Technology Agency of Japanese Government. The method described in ''Radioactivity Survey Data in Japan No. 43 (NIRS-RSD-43, 1977) was applied to the analysis of these two radionuclides in samples. (author)

  9. Comparative Study of Antibacterial Activities of the Fresh and Dried ...

    African Journals Online (AJOL)

    The fresh and dried fruit extracts of Capsicum species were screened for antibacterial activities against Staph. aureus, S. typhi and B. subtilis using two assay methods. The filter disk and agar plate diffusion were the assay methods employed in the study. The results of the study revealed that the extracts obtained from the ...

  10. Decomposition of fresh and anaerobically digested plant biomass in soil

    International Nuclear Information System (INIS)

    Moorhead, K.K.; Graetz, D.A.; Reddy, K.R.

    1987-01-01

    Using water hyacinth [Eichhornia crassipes (Mart.) Solms] for waste water renovation produces biomass that must be disposed of. This biomass may be anaerobically digested to produce CH 4 or added to soil directly as an amendment. In this study, fresh and anaerobically digested water hyacinth biomass, with either low or high N tissue content, were added to soil to evaluate C and N mineralization characteristics. The plant biomass was labeled with 15 N before digestion. The fresh plant biomass and digested biomass sludge were freeze-dried and ground to pass a 0.84-mm sieve. The materials were thoroughly mixed with a Kindrick fine sand at a rate of 5 g kg -1 soil and incubated for 90 d at 27 0 C at a moisture content adjusted to 0.01 MPa. Decomposition was evaluated by CO 2 evolution and 15 N mineralization. After 90 d, approximately 20% of the added C of the digested sludges had evolved as CO 2 compared to 39 and 50% of the added C of the fresh plant biomass with a low and high N content, respectively. First-order kinetics were used to describe decomposition stages. Mineralization of organic 15 N to 15 NO 3 - -N accounted for 8% of applied N for both digested sludges at 90 d. Nitrogen mineralization accounted for 3 and 33% of the applied organic N for fresh plant biomass with a low and high N content, respectively

  11. Semi-continuous anaerobic treatment of fresh leachate from ...

    African Journals Online (AJOL)

    A semi-continuous leachate treatment process was developed in the present study. The fresh leachate was obtained from a municipal solid waste transfer station and palm oil mill effluent (POME) sludge was used as sources of anaerobic microbial complex. The semi-continuous treatment of leachate was operated in two ...

  12. Microbiota analysis to reveal temperature abuse of fresh pork

    DEFF Research Database (Denmark)

    Buschhardt, Tasja; Bahl, Martin Iain; Hansen, Tina Beck

    2017-01-01

    whether temperature induced changes in the community composition on fresh meat surfaces can reflect the temperature-history (combination of time and temperature). Sterile pieces of pork were inoculated with a carcass swab homogenate, to which Salmonella was added. Changes in the meat microbiota were...

  13. Huge increases in bacterivores on freshly killed barley roots

    DEFF Research Database (Denmark)

    Christensen, S.; Griffiths, B.; Ekelund, Flemming

    1992-01-01

    Adding fresh roots to intact soil cores resulted in marked increases in microbial and microfaunal activity at the resource islands. Microbial activity increased in two phases following root addition. Respiratory activity and concentration of respiratory enzyme (dehydrogenase) in soil adhering to ...

  14. Color image segmentation to detect defects on fresh ham

    Science.gov (United States)

    Marty-Mahe, Pascale; Loisel, Philippe; Brossard, Didier

    2003-04-01

    We present in this paper the color segmentation methods that were used to detect appearance defects on 3 dimensional shape of fresh ham. The use of color histograms turned out to be an efficient solution to characterize the healthy skin, but a special care must be taken to choose the color components because of the 3 dimensional shape of ham.

  15. Supply chain risks and smallholder fresh produce farmers in the ...

    African Journals Online (AJOL)

    A survey of 52 smallholder fresh produce farmers was conducted in the Gauteng province of South Africa to grasp how risk and its management affect the mainstreaming of smallholder farmers into formal, high-value markets. The study employed a supply chain analysis approach, which focused on the functions and risks ...

  16. 77 FR 10772 - Fresh and Chilled Atlantic Salmon From Norway

    Science.gov (United States)

    2012-02-23

    ... and Chilled Atlantic Salmon From Norway Determination On the basis of the record \\1\\ developed in the... countervailing duty order and antidumping duty order on fresh and chilled Atlantic salmon from Norway would not... and Chilled Atlantic Salmon from Norway: Investigation Nos. 701-TA-302 and 731-TA-454 (Third Review...

  17. Genetic by environment interaction on fresh root yield, dry matter ...

    African Journals Online (AJOL)

    Eighteen yellow-fleshed cassava genotypes and two released white-fleshed clones (check) were evaluated in five locations representing the major cassava growing agroecological zones of Nigeria to access their performance for fresh root yield, dry matter content, total carotene content and genotypes by environment ...

  18. Bacterial spoilage of fresh meat in some selected Lagos markets ...

    African Journals Online (AJOL)

    A study of the bacteria associated with spoilage of fresh meat was carried out. The flora causing spoilage of meat include Alcaligenes liquefaciens, Bacillus subtilis, Clostridium perfringes, Escherichia coli, Klebsiella pneumoniae, Lactobacillus sp., Micrococcus varians, Pseudomonas aeruginosa, Sarcina sp. Serratia ...

  19. Isolation, Characterization and Anti-Inflammatory Property of Thevetia Peruviana

    Directory of Open Access Journals (Sweden)

    R. Thilagavathi

    2010-01-01

    Full Text Available Thevetia peruviana seeds contain glucosides of neriifolin, acetylneriifolin and thevetin. Seed oil distillates of Thevetia peruviana have been found to contain anti-bacterial activity. In the persent work, the fresh flowers of Thevetia peruviana was subjected to phytochemical studies. The results of the study showed that the flowers contain quercetin, kaempferol and quercetin-7-o-galactoside. The structure of the isolated compound was characterized by UV, 1H NMR and 13C NMR spectra. The anti-inflammatory character of the isolated compound was tested by in vitro method and the results of the study revealed that the isolated compound showed a biphasic property.

  20. Bacterial quality and safety of packaged fresh leafy vegetables at the retail level in Finland.

    Science.gov (United States)

    Nousiainen, L-L; Joutsen, S; Lunden, J; Hänninen, M-L; Fredriksson-Ahomaa, M

    2016-09-02

    Consumption of packaged fresh leafy vegetables, which are convenient ready-to-eat products, has increased during the last decade. The number of foodborne outbreaks associated with these products has concurrently increased. In our study, (1) label information, (2) O2/CO2 composition, (3) bacterial quality and (4) safety of 100 fresh leafy vegetables at the retail level were studied in Finland during 2013. Bacterial quality was studied using aerobic bacteria (AB) and coliform bacteria (CB) counts, and searching for the presence of Escherichia coli, Listeria and Yersinia. The safety was studied by the presence of Salmonella, ail-positive Yersinia, stx-positive E. coli (STEC) and Listeria monocytogenes using PCR and culturing. Important label information was unavailable on several packages originating from different companies. The packaging date was missing on all packages and the date of durability on 83% of the packages. Storage temperature was declared on 62% of the packages and 73% of the packages contained information about prewashing. The batch/lot number was missing on 29% of the packages. Very low oxygen (O2) (vegetable samples varying between 6.2 and 10.6 and 4.2-8.3logcfu/g, respectively. In most of the samples, the AB and CB counts exceeded 10(8) and 10(6)cfu/g, respectively. A positive correlation was observed between the AB and CB counts. E. coli was isolated from 15% of the samples and Yersinia from 33%. L. monocytogenes was isolated from two samples and ail-positive Y. enterocolitica in one. Using PCR, STEC was detected in seven samples, and Salmonella and ail-positive Y. enterocolitica in two samples each. The AB and CB mean values of products originating from different companies varied widely. High AB and CB counts and pathogenic bacteria were detected in ready-to-eat products not needing washing before use. Our study shows that the bacterial quality and safety of packaged fresh leafy vegetables is poor and label information on the packages is

  1. Loads and antimicrobial resistance of Campylobacter spp. on fresh chicken meat in Nueva Ecija, Philippines.

    Science.gov (United States)

    Sison, F B; Chaisowwong, W; Alter, T; Tiwananthagorn, S; Pichpol, D; Lampang, K N; Baumann, M P O; Gölz, G

    2014-05-01

    This study was performed to determine the prevalence and to semiquantify Campylobacter spp. on chicken meat samples at 4 selected local wet markets in Nueva Ecija, Philippines, and to determine the antimicrobial resistance patterns of the Campylobacter isolates. Out of 120 chicken meat samples, 57 (47.5%) were Campylobacter spp. positive. The majority of isolated Campylobacter strains were identified as Campylobacter coli (54.4%) and 45.6% as Campylobacter jejuni. Most of these positive samples (52.6%) showed a very high quantitative Campylobacter contamination (most probable number > 2,400/g, lower confidence limit 580/g). For antimicrobial resistance testing, 44 C. coli/jejuni isolates were tested using the agar disk diffusion method. Out of these, 77.3% were resistant to ampicillin, followed by ciprofloxacin (70.4%), tetracycline (54.6%), erythromycin (20.2%), and gentamicin (11.4%). Of the isolates, 36.4% (n = 16) were resistant to 1 antimicrobial agent, 34.1% (n = 15) were resistance to 3 antimicrobial agents, 13.6% (n = 6) to 2 antimicrobial agents, 9.1% (n = 4) to 4 antimicrobial agents, and 6.8% (n = 3) to all 5 antimicrobial agents tested. Our data demonstrate a high contamination of fresh chicken meat with Campylobacter spp. at retail in the Philippines. The detected high Campylobacter prevalences and quantitative loads on chicken meat at retail in the Philippines highlight the need to implement efficient intervention measures along the food chain and to encourage sanitary handling of poultry meat.

  2. Peroxidase production and ligninolytic potentials of fresh water bacteria Raoultella ornithinolytica and Ensifer adhaerens

    Directory of Open Access Journals (Sweden)

    Ayodeji O. Falade

    2017-12-01

    Full Text Available Interest in novel ligninolytic bacteria has remained topical due to, in part, the maneuverability of the bacterial genome. Conversely, the fungal genome lacks the dexterity for similar maneuverability thus, posing challenges in the fungal enzyme yield optimization process. Some impact of this situation includes the inability to commercialize the bio-catalytic process of lignin degradation by fungi. Consequently, this study assessed some fresh water bacteria isolates for ligninolytic and peroxidase properties through the utilization and degradation of model lignin compounds (guaiacol and veratryl alcohol and the decolourization of selected ligninolytic indicator dyes; Azure B (AZB, Remazol Brilliant Blue R (RBBR and Congo Red (CR. Bacterial strains with appreciable ligninolytic and peroxidase production potentials were identified through 16S rDNA sequence analysis and the nucleotide sequences deposited in the GenBank. About 5 isolates were positive for the degradation of both guaiacol (GA and veratryl alcohol (VA thus, accounting for about 17% of the test isolates. Similarly, AZB, RBBR and CR were respectively decolorized by 3, 2 and 5 bacterial strains thus, accounting for 10%, 7% and 17% of the test isolates. Two of the test bacterial strains were able to decolourize AZB, RBBR and CR respectively and these bacterial strains were identified as Raoultella ornithinolytica OKOH-1 and Ensifer adhaerens NWODO-2 with respective accession numbers as KX640917 and KX640918. Upon quantitation of the peroxidase activities; 5250 ± 0.00 U/L was recorded against Raoultella ornithinolytica OKOH-1 and 5833 ± 0.00 U/L against Ensifer adhaerens NWODO-2. The ligninolytic and dye decolourization properties of Raoultella ornithinolytica OKOH-1 and Ensifer adhaerens NWODO-2 marks for novelty particularly, as dyes with arene substituents were decolourized. Consequently, the potentials for the industrial applicability of these test bacterial strains abound as

  3. Phenolic Compounds of Fresh Roots and Rhizomes of Garden Angelica Angelica Archangelica L.

    Directory of Open Access Journals (Sweden)

    R. M. BASHIROVA

    2014-06-01

    Full Text Available Because in the process of drying and storage of plant materials is a change of genuine compounds, we studied the content of phenolic compounds in freshly drawn angelica roots and rhizomes of the drug Angelica archangelica L. samples had been taken on the territory of the Bashkir State University experimental plot a few days before soil freezing. Fresh rhizomes and roots were ground at room temperature and poured with 96% ethanol. The first portion of the extract containing the glycosides was kept at +5 ° C to precipitate the polysaccharides, and the residual plant material was extracted repeatedly during the day of 96% ethanol to isolate coumarins.After two hours, the first portion of the extract containing glycosides was frozen in a refrigerator, and the remaining part was re-extracted by 96% ethanol for coumarin isolation next day. The second portion representing 80% of the ethanol extract was evaporated to a "tar" state under vacuum at room temperature. Then it was dissolved in CHCl3 and placed in the refrigerator. After a day of keeping the chloroform extract at -10°C, its separation into two fractions was observed: a yellow-orange top and a poor-colored bottom.After separation a gas chromatography-mass spectrometer Thermo Finnigan, 800-Finnigan chromatograph and mass spectrometer of high resolution MAT-95XP "Delta" with “Data System” processing were used.In the alcohol fraction (50% 6.45% there were found catechol, methylcatechol 0.61%, 4.23% 2-methoxy-4-vinylphenol, xanthyletine 0.78%, 4.21% osthol, 10.47% 2,2-dimethyl-3,4-dihydro-2H,5H-pyrano[2,3-b]chromen-5-one, 1.35% kvannin, isoangenomalin 0.37%, 0.8% psoralen and 1.32% prangenin.In the colored fractions 0.41 % bergaptol, 0.41 % marmesin, 0.20 % oroselonе, and 0.07 % neobyakangelikol have been found. High levels of ethyl α-D-glucopyranoside amounting to 35 % were observed in this fraction as well. furocoumarin glycosides are entirely possible to be hydrolysed during the

  4. Ethnobotanical survey of usage of fresh medicinal plants in Singapore.

    Science.gov (United States)

    Siew, Yin-Yin; Zareisedehizadeh, Sogand; Seetoh, Wei-Guang; Neo, Soek-Ying; Tan, Chay-Hoon; Koh, Hwee-Ling

    2014-09-29

    The use of medicinal plants in human health has been documented since ancient times and they provide a useful source of new therapeutics. In Singapore, despite the accessibility to modern healthcare, there still exist pockets of the population who choose to use locally grown fresh medicinal plants for health promotion and even therapeutic purposes. However to date, there is no published report of first-hand account of their usage in Singapore. As land is scarce and rapidly used for re-development, such important knowledge may be lost if not properly documented in time. This work safeguards the local folk knowledge, and provides information on common and scarcely reported fresh medicinal plants. The objective of this study is to gather information regarding the usage of fresh medicinal plants in Singapore through face-to-face interviews. Information on demographic data and plant-use methods were collated via face-to-face interviews of 200 fresh medicinal plant users who have used fresh medicinal plants in the last five years. The survey protocol was approved by the National University of Singapore Institutional Review Board and informed consent was obtained from every participant. A total of 414 plants represented by 104 plant species from 44 families were reportedly used by the 200 participants. The five most commonly used plants were Clinacanthus nutans (34 users), Strobilanthes crispus (31 users), Pereskia bleo (25 users), Aloe vera (18 users) and Zingiber officinale (16 users). Leaves were the most commonly used plant part while preparing a decoction was the most common method of preparation. The majority of interviewees used plants for general health purposes and to treat diseases related to the respiratory system and cancer. Our survey has successfully documented the rich wealth of traditional usage and knowledge on 414 fresh medicinal plants grown in Singapore through face-to-face interviews with 200 users. This study will serve as a useful resource for

  5. Microbiology of organic and conventionally grown fresh produce

    Directory of Open Access Journals (Sweden)

    Daniele F. Maffei

    Full Text Available ABSTRACT Fresh produce is a generalized term for a group of farm-produced crops, including fruits and vegetables. Organic agriculture has been on the rise and attracting the attention of the food production sector, since it uses eco-agricultural principles that are ostensibly environmentally-friendly and provides products potentially free from the residues of agrochemicals. Organic farming practices such as the use of animal manure can however increase the risk of contamination by enteric pathogenic microorganisms and may consequently pose health risks. A number of scientific studies conducted in different countries have compared the microbiological quality of produce samples from organic and conventional production and results are contradictory. While some have reported greater microbial counts in fresh produce from organic production, other studies do not. This manuscript provides a brief review of the current knowledge and summarizes data on the occurrence of pathogenic microorganisms in vegetables from organic production.

  6. Effectiveness of Gamma Irradiated Chitosan for Fresh Meat Preservation

    International Nuclear Information System (INIS)

    Zahran, D.A.

    2015-01-01

    Chitosan is a food preservative of natural origin that has drawn the attention of scientists working in the field of radiation processing and natural polymer products development. The effectiveness of 1% chitosan (CS) and chitosan oligosaccharides (COS) (chitosan subjected to γ irradiation, 50 or 100 kGy) dipping to preserve fresh beef slices during refrigerated storage was investigated. The microbiological quality, lipid and color stability were evaluated. The results revealed that dipping in 1% CS, COS 50 and COS 100 significantly (Ρ< 0.05) improved the microbiological quality and reduced lipid oxidation in beef samples compared to the control samples. On day 7, control samples were considered rejected from the microbiological and lipid oxidation point of view. Dipping in 1% COS 100 significantly (p< 0.05) influenced color properties compared with the other groups. It could be concluded that dipping in 1% COS improved quality parameters of fresh beef and could be used in preservation.

  7. Meat and fish freshness inspection system based on odor sensing.

    Science.gov (United States)

    Najam ul Hasan; Ejaz, Naveed; Ejaz, Waleed; Kim, Hyung Seok

    2012-11-09

    We propose a method for building a simple electronic nose based on commercially available sensors used to sniff in the market and identify spoiled/contaminated meat stocked for sale in butcher shops. Using a metal oxide semiconductor-based electronic nose, we measured the smell signature from two of the most common meat foods (beef and fish) stored at room temperature. Food samples were divided into two groups: fresh beef with decayed fish and fresh fish with decayed beef. The prime objective was to identify the decayed item using the developed electronic nose. Additionally, we tested the electronic nose using three pattern classification algorithms (artificial neural network, support vector machine and k-nearest neighbor), and compared them based on accuracy, sensitivity, and specificity. The results demonstrate that the k-nearest neighbor algorithm has the highest accuracy.

  8. Meat and Fish Freshness Inspection System Based on Odor Sensing

    Directory of Open Access Journals (Sweden)

    Hyung Seok Kim

    2012-11-01

    Full Text Available We propose a method for building a simple electronic nose based on commercially available sensors used to sniff in the market and identify spoiled/contaminated meat stocked for sale in butcher shops. Using a metal oxide semiconductor-based electronic nose, we measured the smell signature from two of the most common meat foods (beef and fish stored at room temperature. Food samples were divided into two groups: fresh beef with decayed fish and fresh fish with decayed beef. The prime objective was to identify the decayed item using the developed electronic nose. Additionally, we tested the electronic nose using three pattern classification algorithms (artificial neural network, support vector machine and k-nearest neighbor, and compared them based on accuracy, sensitivity, and specificity. The results demonstrate that the k-nearest neighbor algorithm has the highest accuracy.

  9. Fresh pasta production enriched with Spirulina platensis biomass

    Directory of Open Access Journals (Sweden)

    Ailton Cesar Lemes

    2012-10-01

    Full Text Available The aim of this work was to study the enrichment of Spirulina platensis in wheat flour to prepare fresh pasta to evaluate the green color and nutritional enrichment in addition to functional properties due to the presence of the bioactive compounds in the cyanobacterium. The pastas were evaluated for the centesimal composition, microbiological contamination, sensorial acceptance and technological characteristics such as cooking time, water absorption, volume displacement and loss of solids. The superior protein contents and the satisfactory technological and sensorial attributes compared with the control with no cyanobacterium showed the usefulness of incorporating S. platensis biomass in the fresh pastas. The microbiological quality was in compliance with the legislation in force. The sensorial quality was considered satisfactory (“liked very much” and purchase intention high (“probably would buy”.

  10. Drop testing of the Westinghouse fresh nuclear fuel package

    International Nuclear Information System (INIS)

    Shappert, L.B.; Sanders, C.F.

    1992-01-01

    In recent years, the Westinghouse Columbia Fuel Fabrication Facility has been faced with increasing pressure from utilities that wished to take the fuel in their nuclear power plants to higher burnups. To help accommodate this trend, Westinghouse has determined that it needs the ability to increase the enrichment of the fresh fuel it delivers to its customers. One critical step in this process is to certify a new (Type A, fissile) fresh fuel package design that has the capability to transport fuel with a higher enrichment than was previously available. A prototype package was tested in support of the Safety Analysis Report of the Packaging. This paper provides detailed information on those tests and their results

  11. Chemical interaction of fresh and saline waters with compacted bentonite

    International Nuclear Information System (INIS)

    Muurinen, A.; Lehikoinen, J.; Melamed, A.; Pitkaenen, P.

    1996-01-01

    The interaction of compacted sodium bentonite with fresh and saline ground-water simulant was studied. The parameters varied in the experiments were the compositions of the solutions and oxygen and carbon dioxide content in the surroundings. The main interests of the study were the chemical changes in the experimental solution, bentonite porewater and bentonite together with the microstructural properties of bentonite. The major processes with fresh water were the diffusion of sodium, potassium, sulphate, bicarbonate and chloride from bentonite to the solution, and the diffusion of calcium and magnesium from the solution into bentonite. The major processes in the experiments with saline water were the diffusion of the sodium, magnesium, sulphate and bicarbonate from bentonite into the solution, and the diffusion of calcium from the solution into bentonite

  12. The relaxation phenomena of radicals induced in irradiated fresh mangoes

    International Nuclear Information System (INIS)

    Kikuchi, Masahiro; Morishita, Norio; Kobayashi, Yasuhiko; Ogawa, Hideyuki; Shimoyama, Yuhei; Ukai, Mitsuko

    2009-01-01

    Using the γ-irradiated fresh mangoes followed by freeze-drying and powderization, electron spin resonance spectrometry of specimens was performed. As a result, a strong single peak in the flesh, the pericarp and the seed was observed at g=2.004 and attributed to organic free radicals. When relaxation times of the peak was calculated using the method of Lund et al., T 2 showed dose responses according to increasing doses while T 1 was almost constant. Dose responsibility of the relaxation time T 2 obtained from flesh specimens of the mangoes could be measured regardless of the preservation period of 1 to 9 days following γ-irradiation. Therefore, there might be possible to detect the irradiation treatment of fresh mangoes using relaxation time T 2 . (author)

  13. Reduction of radiation injury of fresh agricultural products by saccharide

    International Nuclear Information System (INIS)

    Todoroki, Setsuko; Hayashi, Toru

    1999-01-01

    Radiation exposure has been paid attention as an alternative technique of methylbromide to protect agricultural products against insects. However, it has been pointed out that radiation at a dose lower than 300-400 Gy necessary for insecticidal effects might produce damages in fresh agricultural products. To reduce such radiation damages, radiation sensitivities of various fresh products were determined to clarify whether sugar treatment is effective for reduction of damages. Further, the timing of the treatment and its influence on the metabolism of agricultural product were investigated. When sucrose was added to a cut flower of chrysanthemum via water before and during irradiation, the withering time of its leaves and flowers was earlier than that of the untreated product, whereas continuous administration of sucrose after radiation exposure caused to lengthen the flower's life and delay the leave's yellowing. Thus, it was indicated that continuous sugar supply after irradiation was effective for prevention of radiation damages. (M.N.)

  14. Fog as a fresh-water resource: overview and perspectives.

    Science.gov (United States)

    Klemm, Otto; Schemenauer, Robert S; Lummerich, Anne; Cereceda, Pilar; Marzol, Victoria; Corell, David; van Heerden, Johan; Reinhard, Dirk; Gherezghiher, Tseggai; Olivier, Jana; Osses, Pablo; Sarsour, Jamal; Frost, Ernst; Estrela, María J; Valiente, José A; Fessehaye, Gebregiorgis Mussie

    2012-05-01

    The collection of fog water is a simple and sustainable technology to obtain fresh water for afforestation, gardening, and as a drinking water source for human and animal consumption. In regions where fresh water is sparse and fog frequently occurs, it is feasible to set up a passive mesh system for fog water collection. The mesh is directly exposed to the atmosphere, and the foggy air is pushed through the mesh by the wind. Fog droplets are deposited on the mesh, combine to form larger droplets, and run down passing into a storage tank. Fog water collection rates vary dramatically from site to site but yearly averages from 3 to 10 l m(-2) of mesh per day are typical of operational projects. The scope of this article is to review fog collection projects worldwide, to analyze factors of success, and to evaluate the prospects of this technology.

  15. Importance of Seed Quality for the Fresh-cut Chain

    DEFF Research Database (Denmark)

    Deleuran, Lise Christina; Olesen, Merete Halkjær; Shetty, Nisha

    2011-01-01

    Seed is the most fundamental input in vegetable production on, which the effectiveness of other inputs and outputs depends. If the seed is of poor quality, the use of other inputs is less successful and will occasionally be irrelevant. A definition of seed quality will depend upon the use for......, which the seed is intended. Viability, vigor, uniformity, purity and health of the seeds are quality parameters important for all production systems. The ideal situation for both fresh produce and fresh cut would be seeds, which germinate uniform at a high percentage and subsequently grow relatively...... fast to the desired size, thus, resulting in leaves of improved quality. It is likely that such leaves will be more able to withstand the rigorous processing that includes harvest, transportation, washing, sanitization, de-watering and packaging. This ideal situation would both be determined...

  16. Determinants of Fresh Fish Purchasing Behavior Among Malaysian Consumers

    OpenAIRE

    Abdullahi Farah Ahmed; Zainalabidin Mohamed and Mohd Mansor Ismail

    2011-01-01

    The study investigates demographic and attitudinal characteristics that can affect the purchase decisions of marine fish among Malaysian consumers. A survey was conducted on Kuala Lumpur households using structured questionnaires. Seven hundred respondents were randomly interviewed with regard to their buying behaviour pattern, attitude and perception on fresh marine fish consumption. The data were analyzed using a logit binary model. It was found that the size and income of the households, g...

  17. Sorption of cobalt and zinc from fresh water and seawater

    International Nuclear Information System (INIS)

    Arnoldus, R.; Weijden, C.H. van der; Das, H.A.

    1977-01-01

    The adsorption of Cosup(II) and Znsup(II) from artificial fresh and sea water on to clay minerals is studied. The adsorption isotherms are measured at pH-values of 7 and 8. The specific adsorption of zinc is higher than that of cobalt. This is compatible with the higher average residence time of cobalt than that of zinc in the oceans

  18. Microbiology of organic and conventionally grown fresh produce

    OpenAIRE

    Maffei, Daniele F.; Batalha, Erika Y.; Landgraf, Mariza; Schaffner, Donald W.; Franco, Bernadette D.G.M.

    2016-01-01

    ABSTRACT Fresh produce is a generalized term for a group of farm-produced crops, including fruits and vegetables. Organic agriculture has been on the rise and attracting the attention of the food production sector, since it uses eco-agricultural principles that are ostensibly environmentally-friendly and provides products potentially free from the residues of agrochemicals. Organic farming practices such as the use of animal manure can however increase the risk of contamination by enteric pat...

  19. [The personalised donation of fresh breastmilk in neonatology].

    Science.gov (United States)

    Humbert, Gwénaëlle

    2016-01-01

    Donation of fresh breastmilk in neonatology is subject to guidelines set out in a 1997 memorandum and recommendations issued in 2005. The results of a survey carried out in 2013 show that practices in this area vary greatly from one neonatology unit to another. There is a clear need to adopt a national consensus regarding the conditions of this donation in neonatology, in order to adapt and standardise practices. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  20. Survival and growth of fresh and stored planting stock.

    Science.gov (United States)

    Robert H. Ruth

    1953-01-01

    Does planting stock that has been kept in storage survive and grow as well as freshly dug stock? This question is important because the ground at a forest nursery may still be frozen when spring planting time arrives in the warmer parts of the region. This means that seedlings for spring planting need to be dug in the fall, kept in cold storage over winter, and shipped...

  1. Nutrients, phytochemicals, fungal flora and aflatoxin in fresh and ...

    African Journals Online (AJOL)

    In this study, the effect of salting on the pH, phytochemicals, fungal flora and nutrient composition of Vernonia amygdalina leaves was investigated. There was a decrease in pH from 5.88 for the fresh, to 5.80, 5.73, 5.24, and 5.02 for the light brined, light salted, heavy salted and light brine + vinegar treated leaves, ...

  2. Muscle characteristics only partially explain color variations in fresh hams.

    Science.gov (United States)

    Stufft, K; Elgin, J; Patterson, B; Matarneh, S K; Preisser, R; Shi, H; England, E M; Scheffler, T L; Mills, E W; Gerrard, D E

    2017-06-01

    Fresh hams display significant lean color variation that persists through further processing and contributes to a less desirable cured product. In an attempt to understand the underlying cause of this color disparity, we evaluated the differences in muscle characteristics and energy metabolites across semimembranosus (SM) muscles differing in color variation. The L* (lightness) and a* (redness) values were highest and lowest (Pham color variation but suggest other factors may mitigate or exacerbate these variances. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. The Environmental Kuznets Curve: Exploring a Fresh Specification

    OpenAIRE

    David Bradford; Rebecca Schlieckert; Stephen H. Shore

    2000-01-01

    Using a new specification, we reanalyze the data on worldwide environmental quality investigated by Gene Grossman and Alan Krueger in a well-known paper on the environmental Kuznets curve (which postulates an inverse U shaped relationship between income level and pollution). The new specification enables us to draw conclusions from fixed effects estimation. In general, we find support for the environmental Kuznets curve for some pollutants and for its rejection in other cases. The fresh speci...

  4. Fresh Air Practices in English and Scottish Homes

    DEFF Research Database (Denmark)

    Hauge, Bettina

    2013-01-01

    This article presents anthropological research results on how and why English and Scottish families use the fresh air from outside into the home (FAFOH). The introducing exchange was often heard in the English and Scottish families visited. Throughout the entire study the opposite only occurred...... in a few cases: The man opening the window, the woman closing it, in her own words: ”so that I stay snug”....

  5. Thermodynamics of saline and fresh water mixing in estuaries

    Science.gov (United States)

    Zhang, Zhilin; Savenije, Hubert H. G.

    2018-03-01

    The mixing of saline and fresh water is a process of energy dissipation. The freshwater flow that enters an estuary from the river contains potential energy with respect to the saline ocean water. This potential energy is able to perform work. Looking from the ocean to the river, there is a gradual transition from saline to fresh water and an associated rise in the water level in accordance with the increase in potential energy. Alluvial estuaries are systems that are free to adjust dissipation processes to the energy sources that drive them, primarily the kinetic energy of the tide and the potential energy of the river flow and to a minor extent the energy in wind and waves. Mixing is the process that dissipates the potential energy of the fresh water. The maximum power (MP) concept assumes that this dissipation takes place at maximum power, whereby the different mixing mechanisms of the estuary jointly perform the work. In this paper, the power is maximized with respect to the dispersion coefficient that reflects the combined mixing processes. The resulting equation is an additional differential equation that can be solved in combination with the advection-dispersion equation, requiring only two boundary conditions for the salinity and the dispersion. The new equation has been confronted with 52 salinity distributions observed in 23 estuaries in different parts of the world and performs very well.

  6. Evaluation of fuel performance for fresh and aged CANDU reactor

    Energy Technology Data Exchange (ETDEWEB)

    Jung, Jong Yeob; Bae, Jun Ho; Park, Joo Hwan [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2012-10-15

    Like all other industrial plants, nuclear power plants also undergo degradations, so called ageing, with their operation time. Accordingly, in the recent safety analysis for a refurbished Wolsong 1 NPP, various ageing effects were incorporated into the hydraulic models of a number of the components in the primary heat transport system for conservatism. The ageing data of thermal-hydraulic components for 11 EFPY of Wolsong 1 were derived by using NUCIRC code based on the site operation data and they were modified to the appropriate input data for CATHENA code which is a thermal hydraulic code for a postulated accident analysis. This paper deals with the ageing effect of the PHTS (primary heat transport system) of CANDU reactor on the fuel performance during the normal operation. Initial conditions for fuel performance analysis were derived from the thermal-hydraulic analysis for both fresh and aged core models. Here, fresh core means a core state just right after the refurbishment and the aged core is 11 EFPY state after the refurbishment of Wolsong 1. The fuel performance was analyzed by using ELESTRES code for both fresh and aged core state and the results were compared in order to verify the ageing effect of CANDU HTS on the fuel performance.

  7. Migration of fresh and cryopreserved human spermatozoa in polyacrylamide gel.

    Science.gov (United States)

    Goldstein, M C; Wix, L S; Foote, R H; Feldschuh, R; Feldschuh, J

    1982-05-01

    The ability of freshly collected and frozen human spermatozoa to migrate in round capillary tubes containing specially formulated polyacrylamide gel was investigated, using 33 ejaculates from 27 donors. Each semen sample was divided; one portion was left undiluted, and the other portion was diluted to 50 x 10(6) sperm/ml. Glycerol was used as the cryoprotectant. The percentage of motile sperm cells was determined before and after freezing. Fresh semen contained a higher percentage of motile cells, which migrated farther than those of cryopreserved-thawed semen. Various correlations between the percentage of motile sperm and migration distance ranged from 0.57 to 0.62. There was a low positive correlation of migration distance with sperm cell concentration per milliliter, r = 0.25 to 0.34; and thus adjusting semen samples to a standard sperm concentration improved the accuracy of the test only slightly. The regression coefficient of migration distance on the percentage of motile sperm in fresh semen was 0.65, indicating that for each 10% increase in sperm motility, migration distance is predicted to increase 6.5 mm. Five batches of polyacrylamide gel gave uniform results, and the application of this stable gel to fertility investigations is discussed.

  8. Is Shape of a Fresh and Dried Leaf the Same?

    Directory of Open Access Journals (Sweden)

    Dominik Tomaszewski

    Full Text Available Plants kept as dried herbarium specimens share many characteristics with their living counterparts, but there are some substantial differences between them. Due to dehydration, leaves of herbarium specimens change not only their mass and colour, but in many cases change their dimensions, too. The present study aimed to determine whether leaf shape changes during the drying process. A total of 794 pairs of fresh and dried leaves or leaflets of 22 plant taxa were studied. The shape of the blades was quantified using elliptic Fourier analysis combined with principal component analysis. In addition, area and mass of the leaves were measured. Statistical tests were applied for comparing fresh and dried leaves. The results indicate that the preservation process of pressing and drying plants for herbarium purposes causes changes in leaf shape. In general, the shape changes were directional. As the shape of fresh and dried plants is different, it is strongly recommended that shape analyses should be performed on datasets containing either of the leaf types.

  9. The use of electron accelerators for fresh fruit irradiation

    International Nuclear Information System (INIS)

    Ferdes, O.; Minea, R.

    2000-01-01

    There are presented the results of tests concerning the effects of accelerated electron-beam to some early fresh fruits like strawberries, cherries, sour-cherries and apples. The irradiation were performed on common varieties, in normal conditions, to the NILPRP-Electron Accelerator Laboratory facility consisting in electron-beam accelerators which have the following parameters: - mean beam current, 5 μA; - electron mean energy approximately, 7 MeV; - pulse period, 3.5 μs. The doses varied between 0.5-3.0 kGy and the dose rate was about 1500 Gy/min. It was determined the fruit shelf life and there were analysed the main organoleptic and nutritional properties, as: size, shape, colour, dry weight, acidity, total and reducing sugars, ascorbic acid content and other. For the electron-beam treated fruits it was pointed out an increase in freshness and shelf life extension by 5-7 days for strawberries and more than two weeks for cherries. Otherwise, for the applied doses, the electron-beam irradiation did not produce any significant changes in the fruit characteristic values. These results lead to the conclusion that the electron accelerators could be successfully used as a technological solution for the fresh fruits processing, in view of shelf life extension. There are presented also some technical and economical considerations on the feasibility of this technology and on the use of electron-beam machines for food irradiation. (authors)

  10. Efficacy of fresh packed red blood transfusion in organophosphate poisoning.

    Science.gov (United States)

    Bao, Hang-Xing; Tong, Pei-Jian; Li, Cai-Xia; Du, Jing; Chen, Bing-Yu; Huang, Zhi-Hui; Wang, Ying

    2017-03-01

    The mortality rate caused by organophosphate (OP) poisoning is still high, even the standard treatment such as atropine and oxime improves a lot. To search for alternative therapies, this study was aimed to investigate the effects of packed red blood cell (RBC) transfusion in acute OP poisoning, and compare the therapeutic effects of RBCs at different storage times.Patients diagnosed with OP poisoning were included in this prospective study. Fresh RBCs (packed RBCs stored less than 10 days) and longer-storage RBCs (stored more than 10 days but less than 35 days) were randomly transfused or not into OP poisoning patients. Cholinesterase (ChE) levels in blood, atropine usage and durations, pralidoxime durations were measured.We found that both fresh and longer-storage RBCs (200-400 mL) significantly increased blood ChE levels 6 hours after transfusion, shortened the duration for ChE recovery and length of hospital stay, and reduced the usage of atropine and pralidoxime. In addition, fresh RBCs demonstrated stronger therapeutic effects than longer-storage RBCs.Packed RBCs might be an alternative approach in patients with OP poisoning, especially during early stages.

  11. Behaviour of some fresh fruits under electron-beam irradiation

    International Nuclear Information System (INIS)

    Ferdes, O.; Stroia, A.L.; Potcoava, A.; Cojocaru, M.; Mihnea, R.; Oproiu, C.

    1994-01-01

    The use of ionizing radiation in preservation of fruits and vegetables is widely recognized. In this paper it is presented a study of the effect of electron-beam irradiation of some fresh, early and perishable fruits, like strawberries, cherries, and sour cherries concerning their shelf-life time extension. The irradiations were performed on common varieties in normal conditions to the IPTRD's electron-beam accelerator (Bucharest-Magurele) having the following parameters: flow current 10 μA, power 60 W and electron mean energy 6.23 MeV. The irradiation doses varied between 0.5-3.0 kGy and the dose rates between 100-1500 Gy/min. It was observed the fruit preservation capability of the treatment and it was analysed the main characteristics as organoleptic properties, weight of dry component, acidity, total and reducing sugars, ascorbic acid content and others. It was evidenced an increase in freshness and shelf-life extension by 5-7 days for strawberries and up to two weeks for cherries without any significant changes in the values of the considered parameters. Otherwise, for the applied doses, the electron-beam irradiation did not produce any significant changes in the values of fruit characteristic parameters. The results lead to the conclusion that the electron-beam irradiation is a good technological solution for fresh fruit processing. (Author) 1 Tab., 7 Refs

  12. Evaluation of Food Freshness and Locality by Odor Sensor

    Science.gov (United States)

    Koike, Takayuki; Shimada, Koji; Kamimura, Hironobu; Kaneki, Noriaki

    The aim of this study was to investigate whether food freshness and locality can be classified using a food evaluation system consisting four SnO2-semiconductor gas sensors and a solid phase column, into which collecting aroma materials. The temperature of sensors was periodically changed to be in unsteady state and thus, the sensor information was increased. The parameters (in quefrency band) were extracted from sensor information using cepstrum analysis that enable to separate superimposed information on sinusoidal wave. The quefrency was used as parameters for principal component and discriminant analyses (PCA and DCA) to detect food freshness and food localities. We used three kinds of strawberries, people can perceive its odors, passed from one to three days after harvest, and kelps and Ceylon tea, people are hardly to perceive its odor, corrected from five areas as sample. Then, the deterioration of strawberries and localities of kelps and Ceylon teas were visually evaluated using the numerical analyses. While the deteriorations were classified using PCA or DCA, the localities were classified only by DCA. The findings indicate that, although odorant intensity influenced the method detecting food quality, the quefrency obtained from odorant information using cepstrum analysis were available to detect the difference in the freshness and the localities of foods.

  13. Physicochemical and microbiological changes in irradiated fresh pork loins

    International Nuclear Information System (INIS)

    Dogbevi, M.K.; Vachon, C.; Lacroix, M.

    1999-01-01

    The effect of γ-irradiation on the physicochemical and microbiological properties of fresh pork was studied. Radiation treatments were carried out under air on fresh pork loins at doses of 2, 4 and 8 kGy and the loins were evaluated for deamidation, solubility, sulphydryl content and surface hydrophobicity. Deamidation was significantly (p⩽0.05) affected by the treatment with 98% deamidation at a dose of 8 kGy. No significant changes (p>0.05) were noted in sulphydryl content under the same conditions. The increase in deamidation resulted in a decrease in hydrophobicity and an increase in protein solubility. γ-irradiation also reduced the number of microorganisms in the meat. Mesophiles were more resistant to the irradiation treatment than psychrotrophs and Pseudomonas. All irradiated pork samples (1 or 3 kGy) had a bacterial count lower that 10(7) CFU/g after 15 days of storage. A minimal dose of 1 kGy was sufficient to increase the shelf life of fresh pork loins although variations in initial pork contamination was found to be the determining factor accounting for the effectiveness of the treatment

  14. Factors influencing the microbial safety of fresh produce: a review.

    Science.gov (United States)

    Olaimat, Amin N; Holley, Richard A

    2012-10-01

    Increased consumption, larger scale production and more efficient distribution of fresh produce over the past two decades have contributed to an increase in the number of illness outbreaks caused by this commodity. Pathogen contamination of fresh produce may originate before or after harvest, but once contaminated produce is difficult to sanitize. The prospect that some pathogens invade the vascular system of plants and establish "sub-clinical" infection needs to be better understood to enable estimation of its influence upon risk of human illness. Conventional surface sanitation methods can reduce the microbial load, but cannot eliminate pathogens if present. Chlorine dioxide, electrolyzed water, UV light, cold atmospheric plasma, hydrogen peroxide, organic acids and acidified sodium chlorite show promise, but irradiation at 1 kGy in high oxygen atmospheres may prove to be the most effective means to assure elimination of both surface and internal contamination of produce by pathogens. Pathogens of greatest current concern are Salmonella (tomatoes, seed sprouts and spices) and Escherichia coli O157:H7 on leafy greens (spinach and lettuce). This review considers new information on illness outbreaks caused by produce, identifies factors which influence their frequency and size and examines intervention effectiveness. Research needed to increase our understanding of the factors influencing microbial safety of fresh produce is addressed. Copyright © 2012 Elsevier Ltd. All rights reserved.

  15. The Effectiveness of FRESH Technique to Teach Descriptive Paragraph

    Directory of Open Access Journals (Sweden)

    Faisal Faisal

    2013-11-01

    Full Text Available This study was aimed at knowing the effectiveness of FRESH technique in teaching descriptive paragraph. The method of this study was quasi experimental method. This study was conducted at one of the state madrasah aliyah in academic year 2012/2013. The total sample of this study was 78 respondents divided into two groups: the control class with no treatment and the experimental class with FRESH technique. The data collection technique used essay tests for pre-test and post-test. The evaluation covered content, organization, vocabulary, style, and mechanic. After data analyses, the students in the experimental class had better achievement than those in the control class. The result of t-test computation was 1.968, with t-table value = 1.664 at the significant level α = 0.05. The t-test result was higher than the value of t-table (1.968 > 1.664. It meant that the hypothesis was accepted or FRESH technique was effective in teaching descriptive paragraph to the students of that school in academic year 2012/2013.

  16. Thermal conductivity of fresh and irradiated U-Mo fuels

    Science.gov (United States)

    Huber, Tanja K.; Breitkreutz, Harald; Burkes, Douglas E.; Casella, Amanda J.; Casella, Andrew M.; Elgeti, Stefan; Reiter, Christian; Robinson, Adam. B.; Smith, Frances. N.; Wachs, Daniel. M.; Petry, Winfried

    2018-05-01

    The thermal conductivity of fresh and irradiated U-Mo dispersion and monolithic fuel has been investigated experimentally and compared to theoretical models. During in-pile irradiation, thermal conductivity of fresh dispersion fuel at a temperature of 150 °C decreased from 59 W/m·K to 18 W/m·K at a burn-up of 4.9·1021 f/cc and further to 9 W/m·K at a burn-up of 6.1·1021 f/cc. Fresh monolithic fuel has a considerably lower thermal conductivity of 15 W/m·K at a temperature of 150 °C and consequently its decrease during in-pile irradiation is less steep than for dispersion fuel. For a burn-up of 3.5·1021 f/cc of monolithic fuel, a thermal conductivity of 11 W/m·K at a temperature of 150 °C has been measured by Burkes et al. (2015). The difference of decrease for both fuels originates from effects in the matrix that occur during irradiation, like for dispersion fuel the gradual disappearance of the Al matrix with increased burn-up and the subsequent growth of an interaction layer (IDL) between the U-Mo fuel particle and Al matrix and subsequent matrix hardening. The growth of fission gas bubbles and the decomposition of the U-Mo crystal lattice also affect both dispersion and monolithic fuel.

  17. Fresh fuel pre-heating device in reactor facility

    International Nuclear Information System (INIS)

    Samejima, Asakuni.

    1988-01-01

    Purpose: To simplify the structure of a fresh nuclear fuel pre-heating device and improve the reliability to gas supply. Constitution: Fresh fuels taken out from a fresh fuel stredge rack and contained in a fuel strage pipe of a fuel transportation cask are pre-heated at the pre-stage of transfer by sending heating gases from the outside. Gas outlet pipes of the device are led out from the lower portion of the strage pipe, disposed side by side at the top of the strage pipe and opened upwardly. Further, gas supply pipes are connected to the inside of a movable guiding cylinder on the side of the floor surface and the opening end of return pipes are opposed to the exit opening end of the strage pipe. In such a constitution, a gas recycling loop can be formed between the strage pipe and the gas heating device by way of the movable guiding cylinder only by the operation of combining the fuel strage pipe of the transportation cask and the movable guiding pipe disposed on the side of the floor surface. Thus, the coupling structure is facilitated, the connection operation can surely be conducted to improve the reliability as compared with the conventional case. (Horiuchi, T.)

  18. Eat fresh vegetables, fruit, and whole grain products | NIH MedlinePlus the Magazine

    Science.gov (United States)

    ... please turn Javascript on. Feature: Diverticulitis "Eat fresh vegetables, fruit, and whole grain products." Past Issues / Winter 2010 ... or the diverticulitis. I once again eat fresh vegetables and fruit and whole grain products. My two episodes of ...

  19. Promoting seafood consumption: An evaluation of the Danish campaign for fresh fish

    DEFF Research Database (Denmark)

    Scholderer, Joachim; Grunert, Klaus G.

    2003-01-01

    was that preparing a tasty meal from fresh fish was actually not difficult. In supplementary materials consumers were provided with recipes for fresh fish. At the same time, major retail chains made MAP-packaged fresh fish filets available in Danish supermarkets. The influence of family expectations was harnessed......Before and after the 3-year generic advertising campaign for fresh fish in Denmark, representative consumer samples were surveyed with regard to their attitudes towards fresh fish, perceived family norms, availability of fresh fish in shops, meal preparation skills, intentions to buy fresh fish......, and actual consumption frequencies. In the pre-campaign survey (effective N = 641), significant determinants of consumption were availability in shops, meal preparation skills, and intentions to buy fresh fish. The campaign was especially designed to conquer these barriers. The key proposition...

  20. Adaptations in the glucose metabolism of procyclic Trypanosoma brucei isolates from Tsetse flies and during differentiation of bloodstream forms.

    NARCIS (Netherlands)

    van Grinsven, K.W.A.; van den Abbeele, J.; van den Bossche, P.; van Hellemond, J.J.; Tielens, A.G.M.

    2009-01-01

    Procyclic forms of Trypanosoma brucei isolated from the midguts of infected tsetse flies, or freshly transformed from a strain that is close to field isolates, do not use a complete Krebs cycle. Furthermore, short stumpy bloodstream forms produce acetate and are apparently metabolically preadapted

  1. 75 FR 38986 - Fresh Garlic From the People's Republic of China: Initiation of New Shipper Reviews

    Science.gov (United States)

    2010-07-07

    ... DEPARTMENT OF COMMERCE International Trade Administration [A-570-831] Fresh Garlic From the People... on fresh garlic from the People's Republic of China (PRC), meet the statutory and regulatory... garlic from the PRC was published on November 16, 1994. See Antidumping Duty Order: Fresh Garlic from the...

  2. 76 FR 78694 - Fresh Garlic From China; Scheduling of an expedited five-year review

    Science.gov (United States)

    2011-12-19

    ... INTERNATIONAL TRADE COMMISSION [Investigation No. 731-TA-683 (Third Review)] Fresh Garlic From... whether revocation of the antidumping duty order on fresh garlic from China would be likely to lead to... submitted by the Fresh Garlic Producers Association and its individual members Christopher Ranch L.L.C., The...

  3. 77 FR 266 - Fresh Garlic From the People's Republic of China: Initiation of New Shipper Reviews

    Science.gov (United States)

    2012-01-04

    ... DEPARTMENT OF COMMERCE International Trade Administration [A-570-831] Fresh Garlic From the People... determined that two requests for a new shipper review (NSR) under the antidumping duty order on fresh garlic.... SUPPLEMENTARY INFORMATION: Background The Department published the antidumping duty order on fresh garlic from...

  4. 77 FR 22467 - Common Crop Insurance Regulations; Fresh Market Tomato (Dollar Plan) Crop Provisions

    Science.gov (United States)

    2012-04-16

    ...-0006] RIN 0563-AC32 Common Crop Insurance Regulations; Fresh Market Tomato (Dollar Plan) Crop... Insurance Corporation (FCIC) finalizes the Common Crop Insurance Regulations, Fresh Market Tomato (Dollar... Common Crop Insurance Regulations (7 CFR part 457), Fresh Market Tomato (Dollar Plan) Crop Provisions...

  5. 7 CFR 457.148 - Fresh market pepper crop insurance provisions.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Fresh market pepper crop insurance provisions. 457.148... pepper crop insurance provisions. The fresh market pepper crop insurance provisions for the 1999 and... Fresh Market Pepper Crop Provisions If a conflict exists among the policy provisions, the order of...

  6. On-line fresh-cut lettuce quality measurement system using hyperspectral imaging

    Science.gov (United States)

    Lettuce, which is a main type of fresh-cut vegetable, has been used in various fresh-cut products. In this study, an online quality measurement system for detecting foreign substances on the fresh-cut lettuce was developed using hyperspectral reflectance imaging. The online detection system with a s...

  7. 7 CFR 457.129 - Fresh market sweet corn crop insurance provisions.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Fresh market sweet corn crop insurance provisions. 457... sweet corn crop insurance provisions. The fresh market sweet corn crop insurance provisions for the 2008... Reinsured Policies Fresh Market Sweet Corn Crop Provisions 1. Definitions Allowable cost.—The dollar amount...

  8. Novel technologies for decontamination of fresh and minimally processed fruits and vegetables

    Science.gov (United States)

    The complex challenges facing producers and processors of fresh produce require creative applications of conventional treatments and innovative approaches to develop entirely novel treatments. The varied nature of fresh and fresh-cut produce demands solutions that are adapted and optimized for each ...

  9. 75 FR 32370 - Final Results of Antidumping Duty Changed Circumstances Review: Fresh and Chilled Atlantic Salmon...

    Science.gov (United States)

    2010-06-08

    ... Duty Changed Circumstances Review: Fresh and Chilled Atlantic Salmon from Norway AGENCY: Import... Duty Changed Circumstances Review: Fresh and Chilled Atlantic Salmon from Norway SUMMARY: On August 5... antidumping order on fresh and chilled Atlantic Salmon from Norway and preliminarily determined that Nordic...

  10. 78 FR 25692 - Notice of Decision To Authorize the Importation of Fresh Barhi Dates From Israel

    Science.gov (United States)

    2013-05-02

    ...] Notice of Decision To Authorize the Importation of Fresh Barhi Dates From Israel AGENCY: Animal and Plant... authorize the importation into the United States of fresh dates of the cultivar Barhi from Israel. Based on... weeds via the importation of fresh dates of the cultivar Barhi from Israel. In addition, based on the...

  11. Mexican unpasteurised fresh cheeses are contaminated with Salmonella spp., non-O157 Shiga toxin producing Escherichia coli and potential uropathogenic E. coli strains: A public health risk.

    Science.gov (United States)

    Guzman-Hernandez, Rosa; Contreras-Rodriguez, Araceli; Hernandez-Velez, Rosa; Perez-Martinez, Iza; Lopez-Merino, Ahide; Zaidi, Mussaret B; Estrada-Garcia, Teresa

    2016-11-21

    Fresh cheeses are a main garnish of Mexican food. Consumption of artisanal fresh cheeses is very common and most of them are made from unpasteurised cow milk. A total of 52 fresh unpasteurised cheeses of five different types were purchased from a variety of suppliers from Tabasco, Mexico. Using the most probable number method, 67% and 63% of samples were positive for faecal coliforms and E. coli, respectively; revealing their low microbiological quality. General hygienic conditions and practices of traditional cheese manufacturers were poor; most establishments had unclean cement floors, all lacked windows and doors screens, and none of the food-handlers wore aprons, surgical masks or bouffant caps. After analysing all E. coli isolates (121 strains) for the presence of 26 virulence genes, results showed that 9 (17%) samples were contaminated with diarrheagenic E. coli strains, 8 harboured non-O157 Shiga toxin producing E. coli (STEC), and one sample contained both STEC and diffusely adherent E. coli strains. All STEC strains carried the stx1 gene. Potential uropathogenic E. coli (UPEC) strains were isolated from 15 (29%) samples; the most frequent gene combination was fimA-agn43. Two samples were contaminated with Salmonella. The results demonstrated that unpasteurised fresh cheeses produced in Tabasco are of poor microbiological quality and may frequently harbour foodborne pathogens. Food safety authorities in Mexico need to conduct more rigorous surveillance of fresh cheeses. Furthermore, simple and inexpensive measures as establishing programs emphasizing good hand milking practices and hygienic manufacturing procedures may have a major effect on improving the microbiological quality of these food items. Copyright © 2016 Elsevier B.V. All rights reserved.

  12. Microbiological quality and antimicrobial resistance characterization of Salmonella spp. in fresh milk value chains in Ghana.

    Science.gov (United States)

    Parry-Hanson Kunadu, Angela; Holmes, Mark; Miller, Eric L; Grant, Andrew J

    2018-07-20

    Consumer perception of poor hygiene of fresh milk products is a major barrier to promotion of milk consumption as an intervention to alleviate the burden of malnutrition in Ghana. Fresh milk is retailed raw, boiled, or processed into unfermented cheese and spontaneously fermented products in unlicensed outlets. In this study, we have determined microbiological quality of informally retailed fresh milk products and characterized the genomic diversity and antimicrobial resistance (AMR) patterns of non-typhoidal Salmonella (NTS) in implicated products. A total of 159 common dairy products were purchased from five traditional milk markets in Accra. Samples were analysed for concentrations of aerobic bacteria, total and fecal coliforms, Escherichia coli, staphylococci, lactic acid bacteria and yeast and moulds. The presence of Salmonella, E. coli O157:H7, Listeria monocytogenes and Staphylococcus aureus were determined. AMR of Salmonella against 18 antibiotics was experimentally determined. Genome sequencing of 19 Salmonella isolates allowed determination of serovars, antigenic profiles, prediction of AMR genes in silico and inference of phylogenetic relatedness between strains. Raw and heat-treated milk did not differ significantly in overall bacterial quality (P = 0.851). E. coli O157:H7 and Staphylococcus aureus were present in 34.3% and 12.9% of dairy products respectively. Multidrug resistant (MDR) Salmonella enterica serovars Muenster and Legon were identified in 11.8% and 5.9% of unfermented cheese samples respectively. Pan genome analysis revealed a total of 3712 core genes. All Salmonella strains were resistant to Trimethoprim/Sulfamethoxazole, Cefoxitin, Cefuroxime Axetil and Cefuroxime. Resistance to Chloramphenicol (18%) and Ciprofloxacin (100%), which are first line antibiotics used in treatment of NTS bacteremia in Ghana, was evident. AMR was attributed to presence and/or mutations in the following genes: golS, sdiA for cephalosporins, aac(6')-Iy, ant

  13. Conceptual Assessment of a Fresh Fuel Transport Package for KJRR

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Ju-Chan; Choi, W. S.; Bang, K. S.; Yu, S. H.; Park, J. S.; Yang, Y. Y. [KAERI, Daejeon (Korea, Republic of)

    2016-05-15

    The IAEA and domestic regulations stipulate that the fissile material transport package be subjected to the cumulative effects of a 9 m drop, 1 m puncture, 800 ℃ thermal and water leakage tests. A fissile material transport package should be maintained the subcriticality during the normal and accident conditions for contingency of leakage of water into or out of package, rearrangement of the contents, reduction of spaces and temperature changes. KAERI has been developing a fresh fuel transport package for Kijang research reactor (KJRR). This paper describes a conceptual design and preliminary safety analysis of the transport package for KJRR. The transport package was designed for shipment of a fresh fuel and a FM (Fission Molybdenum) target. Low-enriched uranium (LEU) of U-Mo fuel with U-235 enrichment of 19.75 w/o is used as a research reactor fuel. And LEU of UAlx-Al with U-235 enrichment of 19.75 w/o is used as a FM target material. The transport package was designed for shipment of a fresh fuel and a FM target. Safety analyses were conducted on all areas, including criticality, structural, and thermal fields. In the criticality analysis, effective neutron multiplication factors were below the criticality safety limit. In the structural analysis, the maximum stress satisfied the stress requirement stipulated in the ASME code. After 9 m free drop and 1 m puncture test, there was no significant deformation of fuel basket to cause a criticality. In the thermal analysis, the maximum temperatures at each part were lower than the allowable values.

  14. TECHNOLOGY OF FRESH HERBS STORAGE USING HYDROGEL AND ANTIOXIDANT COMPOSITION

    Directory of Open Access Journals (Sweden)

    Olesia PRISS

    2017-12-01

    Full Text Available There is a stable consumer demand for fresh culinary herbs. Also, the greenery contains a large number of valuable phytonutrients. Despite high efficiency and increasing annual production of fresh herbs, the problem of preserving their quality in the post-harvest period remains unresolved. Because of the high specific surface area of evaporation, in the green crops droop quickly, they lose their marketable quality, and, as a result, the level of profitability of greenery production in general is being reduced. It is necessary to use new effective approaches to leafy greens storage in order to reduce product losses during transportation and storage. For example, agrarian hydrogel can be used for storage of greenery. Hydrogel is an acrylic potassium polymer that is non-toxic and has a high environmental standard. The hydrogel granules can absorb up to 250 times more moisture than their weight. We propose the following procedure as the method of greenery preservation: the greens are packed in bundles and put in sticks in polyethylene bags with a fastener, pre-filled with hydrogel solutions. The storage temperature is maintained optimally for each species of fresh herbs, the relative humidity is 95 ± 3%. Usage of the proposed method allows obtaining environment-friendly products, preserving their high biological value and increasing the shelf life. The accumulation of peroxide products, which cause physiological disorders, is inhibited as the result of such storage. The use of hydrogel reduces the natural loss of mass by 10% as compared with the control. Duration of greenery storage increases by 30 days.

  15. Consumer attitudes and preferences for fresh market tomatoes.

    Science.gov (United States)

    Oltman, A E; Jervis, S M; Drake, M A

    2014-10-01

    This study established attractive attributes and consumer desires for fresh tomatoes. Three focus groups (n = 28 participants) were conducted to explore how consumers perceived tomatoes, including how they purchased and consumed them. Subsequently, an Adaptive Choice Based Conjoint (ACBC) survey was conducted to understand consumer preferences toward traditional tomatoes. The ACBC survey with Kano questions (n = 1037 consumers in Raleigh, NC) explored the importance of color, firmness, size, skin, texture, interior, seed presence, flavor, and health benefits. The most important tomato attribute was color, then juice when sliced, followed by size, followed by seed presence, which was at parity with firmness. An attractive tomato was red, firm, medium/small sized, crisp, meaty, juicy, flavorful, and with few seeds. Deviations from these features resulted in a tomato that was rejected by consumers. Segmentations of consumers were determined by patterns in utility scores. External attributes were the main drivers of tomato liking, but different groups of tomato consumers exist with distinct preferences for juiciness, firmness, flavor, and health benefits. Conjoint analysis is a research technique that collects a large amount of data from consumers in a format designed to be reflective of a real life market setting and can be combined with qualitative insight from focus groups to gain information on consumer consumption and purchase behaviors. This study established that the most important fresh tomato attributes were color, amount of juice when sliced, and size. Distinct consumer clusters were differentiated by preference for color/appearance, juiciness and firm texture. Tomato growers can utilize the results to target attributes that drive consumer choice for fresh tomatoes. © 2014 Institute of Food Technologists®

  16. The traveller: a new look for PWR fresh fuel packages

    International Nuclear Information System (INIS)

    Bayley, B.; Stilwell, W.E.; Kent, N.A.

    2004-01-01

    The Traveller PWR fresh fuel shipping package represents a radical departure from conventional PWR fuel package designs. This paper follows the development effort from the establishment of goals and objectives, to intermediate testing and analysis, to final testing and licensing. The discussion starts with concept origination and covers the myriad iterations that followed until arriving at a design that would meet the demanding licensing requirements, last for 30 years, and would be easy to load and unload fuel, easy to handle, inexpensive to manufacture and transport, and simple and inexpensive to maintain

  17. BATCH ANAEROBIC TREATMENT OF FRESH LEACHATE FROM TRANSFER STATION

    Directory of Open Access Journals (Sweden)

    SEYED MOHAMMAD DARA GHASIMI

    2008-12-01

    Full Text Available Leachate from transfer station requires treatment before being discharged into the environment to avoid surface and underground water contamination. Various factors such as waste composition, availability of oxygen and moisture, designing and controlling of transfer station operations have been shown to affect the composition of the leachate. The high COD, BOD, ammonia nitrogen (NH3-N and heavy metals contents of fresh leachate are the main problems faced by leachate treatment operators. The result of the present study indicated that this process reduced the COD content by 43%.The average removal efficiencies of BOD5, TS, TSS, and VSS were 80, 49, 37 and 39 %, respectively.

  18. Ground Zero/Fresh Kills: Cataloguing Ruins, Garbage, and Memory

    OpenAIRE

    Cinzia Scarpino

    2011-01-01

    The aim of this paper is to show how the rise and fall of the Twin Towers can be read in relation to the rise and fall of the Staten Island Fresh Kills landfill, how their destinies were entwined from the start, and how the immediate cultural response to the collapse of the former and the closing of the latter recurred to the form of catalogues of objects, words, and images. From this angle it will be possible to posit the events within a larger, if somewhat unusual, cultural frame encompassi...

  19. Shelf-life extension of fresh chicken through radurisation

    International Nuclear Information System (INIS)

    Niemand, J.G.; Van der Linde, H.J.

    1982-01-01

    The article discusses the shelf-life extension of fresh chicken through radurization. In order to assess the potential of this process on the South African market, a detailed investigation was carried out to determine the shelf-life extension under local conditions. The following aspects were investigated; 1) reduction of bacterial numbers at different radurisation doses; 2) influence of storage temperature on shelf-life and 3) the elimination of Salmonella. Organoleptic testing was carried out on poultry radurised to doses of 3, 5, 7,5 and 10 kGy as well as on non-radurised controls

  20. Consumer Willingness to Pay for Quality Attributes of Fresh Seafood

    DEFF Research Database (Denmark)

    Nguyen, Thong Tien; Haider, Wolfgang; Solgaard, Hans Stubbe

    2015-01-01

    We applied a labeled choice experiment (LCE) to investigate consumer demand and choice behavior for fresh seafood in a retail market. The LCE was conducted for a large number of seafood alternatives (i.e., seafood species) labeled by the respective seafood name (e.g., cod, salmon, mussels......). Consumer heterogeneity in preference was expressed by estimating a labeled latent class model with alternative-specific effects, which varies choice probability and model parameters over seafood alternatives and across classes. The willingness to pay (WTP) for extrinsic attributes (e.g., product form...

  1. Nanomaterials for fresh-keeping and sterilization in food preservation.

    Science.gov (United States)

    Liu, Dongfang; Gu, Ning

    2009-06-01

    Food sterilizing and antistaling technologies are very important to the public's health and safety and have been attracting more and more attentions. In the past several years, new development chance was created by the introduction of nanomaterials to this critical field. Nanomaterials possess lots of outstanding properties, such as unique quantum size effect, large surface area and catalytic properties, which jointly facilitate high effective fresh-keeping, and thus were considered as promising materials in food sterilization and antistale. This review article focuses on the patented applications of nanomaterials as food biocidal agents, bacteriostatic agents, catalysts and carriers for antistaling agents.

  2. Effect of irradiation on quality of fresh fruits and vegetables

    International Nuclear Information System (INIS)

    Basbayraktar, V.; Gueclue, H.

    2009-01-01

    There is an increasing trend for the consumption of fresh fruits and vegetables in developing and developed countries. However, they carry the potential risk of contamination from soil, irrigation water and/or manure and result in food borne outbreaks. Since they are consumed without any treatments, they are the potential sources of contamination. Irradiation is a physical food preservation method; foods are exposed to low doses of ionizing radiation for sterilization or preservation purposes. Irradiation treatment has been shown to effectively enhance the microbial quality and increase the shelf-life by 3-5 times. Also, this treatment reduces the post harvest losses and controls the insects.

  3. Manufacturing and Construction of Fresh Fuel Storage Rack for a Research Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Oh, Jinho; Lee, Sangjin; Lee, Jongmin; Ryu, Jeong-Soo [KAERI, Daejeon (Korea, Republic of)

    2016-05-15

    The objective of this study is to provide the manufacturing and construction information regarding the Fresh Fuel Storage Rack (FFSR). The main function of a FFSR is to store and protect the 2 core new fuel assemblies for the operation of the research reactor. The fresh fuel assemblies are stored in a rack made of stainless steel and the storage rack is installed in the fresh fuel storage room. The fresh fuel facility provides fresh fuel assemblies with dry storage space. General design requirements of the fresh fuel storage facilities are given in the ANSI-57.3. Design, manufacturing, and construction of the fresh fuel storage rack are introduced. The analysis is performed to confirm the structural intensity of the fresh fuel storage rack under the seismic loads. The fresh fuel storage rack designed for storage of fresh fuel assemblies should be manufactured and installed with consideration of predicted number of fresh fuel assemblies, structural integrity, resistivity to corrosion and radiation, cleaning, and workability.

  4. Manufacturing and Construction of Fresh Fuel Storage Rack for a Research Reactor

    International Nuclear Information System (INIS)

    Oh, Jinho; Lee, Sangjin; Lee, Jongmin; Ryu, Jeong-Soo

    2016-01-01

    The objective of this study is to provide the manufacturing and construction information regarding the Fresh Fuel Storage Rack (FFSR). The main function of a FFSR is to store and protect the 2 core new fuel assemblies for the operation of the research reactor. The fresh fuel assemblies are stored in a rack made of stainless steel and the storage rack is installed in the fresh fuel storage room. The fresh fuel facility provides fresh fuel assemblies with dry storage space. General design requirements of the fresh fuel storage facilities are given in the ANSI-57.3. Design, manufacturing, and construction of the fresh fuel storage rack are introduced. The analysis is performed to confirm the structural intensity of the fresh fuel storage rack under the seismic loads. The fresh fuel storage rack designed for storage of fresh fuel assemblies should be manufactured and installed with consideration of predicted number of fresh fuel assemblies, structural integrity, resistivity to corrosion and radiation, cleaning, and workability

  5. Study on storage efficiency of the fresh food e-commerce

    Science.gov (United States)

    Wang, Xin; Sun, Jie; Li, Huihui

    2017-06-01

    As the last cake in the area of e-commerce industry, the temperature of fresh food e-commerce is always rising starting from about 2014. This paper is based on the imperfection that the existing study about fresh food e-commerce is lack of studies on storage efficiency. And we took some variables in this paper such as consumers’ satisfaction and length for preservation and storage time. On this basis we built the model of storage efficiency of fresh food e-commerce. We find that as the development of fresh food e-commerce, the fresh food e-commerce enterprise will pay more attention to the consumers’ satisfaction. They can take some effective ways like reducing the wastage of fresh food and lengthening the refreshing time of fresh food and so on.

  6. Modified atmosphere packaging of fresh meats : sudden partial adaption caused an increase in sustainability of Dutch supply chains of fresh meats

    NARCIS (Netherlands)

    Thoden Van Velzen, E.U.; Linnemann, A.R.

    2008-01-01

    In the past decade, a major transition evolved in the Dutch fresh meat industry with ramifications for the entire meat business. In 1995, more than 95% of all fresh meat products for consumers were either sold loose or packed in the traditional way, i.e. on a white styrofoam tray with stretch wrap.

  7. Analysing PKCS#11 Key Management APIs with Unbounded Fresh Data

    Science.gov (United States)

    Fröschle, Sibylle; Steel, Graham

    We extend Delaune, Kremer and Steel’s framework for analysis of PKCS#11-based APIs from bounded to unbounded fresh data. We achieve this by: formally defining the notion of an attribute policy; showing that a well-designed API should have a certain class of policy we call complete; showing that APIs with complete policies may be safely abstracted to APIs where the attributes are fixed; and proving that these static APIs can be analysed in a small bounded model such that security properties will hold for the unbounded case. We automate analysis in our framework using the SAT-based security protocol model checker SATMC. We show that a symmetric key management subset of the Eracom PKCS#11 API, used in their ProtectServer product, preserves the secrecy of sensitive keys for unbounded numbers of fresh keys and handles, i.e. pointers to keys. We also show that this API is not robust: if an encryption key is lost to the intruder, SATMC finds an attack whereby all the keys may be compromised.

  8. Recent developments in smart freezing technology applied to fresh foods.

    Science.gov (United States)

    Xu, Ji-Cheng; Zhang, Min; Mujumdar, Arun S; Adhikari, Benu

    2017-09-02

    Due to the increased awareness of consumers in sensorial and nutritional quality of frozen foods, the freezing technology has to seek new and innovative technologies for better retaining the fresh like quality of foods. In this article, we review the recent developments in smart freezing technology applied to fresh foods. The application of these intelligent technologies and the associated underpinning concepts have greatly improved the quality of frozen foods and the freezing efficiency. These technologies are able to automatically collect the information in-line during freezing and help control the freezing process better. Smart freezing technology includes new and intelligent technologies and concepts applied to the pretreatment of the frozen product, freezing processes, cold chain logistics as well as warehouse management. These technologies enable real-time monitoring of quality during the freezing process and help improve product quality and freezing efficiency. We also provide a brief overview of several sensing technologies used to achieve automatic control of individual steps of freezing process. These sensing technologies include computer vision, electronic nose, electronic tongue, digital simulation, confocal laser, near infrared spectroscopy, nuclear magnetic resonance technology and ultrasound. Understanding of the mechanism of these new technologies will be helpful for applying them to improve the quality of frozen foods.

  9. RESEARCH METHODOLOGY ON NATURAL NUTRITION OF FRESH-WATER FISH

    Directory of Open Access Journals (Sweden)

    Marina Piria

    2001-03-01

    Full Text Available This paper offers the entire review on the research methodology in natural nutrition of fresh-water fish. The data on fresh-water fish nutrition, particularly on fish of lower economic value, is inadequate. Reviewing the literature on assesment of nutritional parameters, the authors obviously use differenet approaches and methods. This paper is about most frequently used parameteres in qualitative and quantitative analysis. The qualitative analysis of food structure is the overall list of determinable taxa (mostlyu species and genera. The quantitative analysis comprises the assessment of particular nutritional categories by nutritional indices and coefficients. Bio-identification and numeric data processing can have numerous drawbacsk such as effect of regurgitation or the degree of digestion of the prey. The analyses of those effects proceed through statistical data processing in order to include spatial distribution of certain prey categories as well. The importance of this data is to determine the nutritional needs of potential species for culture as well as to come up with new insights on a particular aquatic ecosystem.

  10. Bacterial communities of fresh goat meat packaged in modified atmosphere.

    Science.gov (United States)

    Carrizosa, Elia; Benito, María José; Ruiz-Moyano, Santiago; Hernández, Alejandro; Villalobos, Maria Del Carmen; Martín, Alberto; Córdoba, María de Guía

    2017-08-01

    The objective of this work was to study the growth and development of fortuitous flora and food pathogens in fresh goat meat packaged under modified atmospheres containing two different concentrations of CO 2 . Meat samples were stored at 10 °C under two different modified-atmosphere packing (MAP) conditions: treatment A had 45% CO 2  + 20% O 2  + 35% N 2 and treatment B had 20% CO 2  + 55% O 2  + 25% N 2 . During 14 days of storage, counts of each bacterial group and dominant species identification by 16S rRNA gene sequencing were performed to determine the microbial diversity present. The MAP condition used for treatment A was a more effective gas mixture for increasing the shelf life of fresh goat meat, significantly reducing the total number of viable bacteria and enterobacteria counts. Members of the Enterobacteriaceae family were the most common contaminants, although Hafnia alvei was dominant in treatment A and Serratia proteamaculans in treatment B. Identification studies at the species level showed that different microorganisms develop under different storage conditions, reflecting the importance of gas composition in the modified atmosphere on the bacterial community. This work provides new insights into the microbial changes of goat meat storage under different MAP conditions, which will be beneficial for the meat industry. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Quality and Acceptability of Meat Nuggets with Fresh Gel

    Directory of Open Access Journals (Sweden)

    V. Rajkumar

    2016-05-01

    Full Text Available Aloe vera has been used worldwide for pharmaceutical, food, and cosmetic industries due to its wide biological activities. However, quality improvement of low fat meat products and their acceptability with added Aloe vera gel (AVG is scanty. The aim of this study was to explore the feasibility of using fresh AVG on physicochemical, textural, sensory and nutritive qualities of goat meat nuggets. The products were prepared with 0%, 2.5%, and 5% fresh AVG replacing goat meat and were analyzed for proximate composition, physicochemical and textural properties, fatty acid profile and sensory parameters. Changes in lipid oxidation and microbial growth of nuggets were also evaluated over 9 days of refrigerated storage. The results showed that AVG significantly (p<0.05 decreased the pH value and protein content of meat emulsion and nuggets. Product yield was affected at 5% level of gel. Addition of AVG in the formulation significantly affected the values of texture profile analysis. The AVG reduced the lipid oxidation and microbial growth in nuggets during storage. Sensory panelists preferred nuggets with 2.5% AVG over nuggets with 5% AVG. Therefore, AVG up to 2.5% level could be used for quality improvement in goat meat nuggets without affecting its sensorial, textural and nutritive values.

  12. Ground Zero/Fresh Kills: Cataloguing Ruins, Garbage, and Memory

    Directory of Open Access Journals (Sweden)

    Cinzia Scarpino

    2011-09-01

    Full Text Available The aim of this paper is to show how the rise and fall of the Twin Towers can be read in relation to the rise and fall of the Staten Island Fresh Kills landfill, how their destinies were entwined from the start, and how the immediate cultural response to the collapse of the former and the closing of the latter recurred to the form of catalogues of objects, words, and images. From this angle it will be possible to posit the events within a larger, if somewhat unusual, cultural frame encompassing the history of two different yet complementary symbols of New York up to 2001 (the WTC and Fresh Kills. From Don DeLillo’s Underworld (1997 and Falling Man (2007 through Holman, Steve Zeitlin e Joe Dobkin’s Crisis (2001-2002; from Art Spiegelman’s In the Shadows of No Tower (2004 to Artists Respond’s 9-11 (2002; from the New York Times to Bearing Witness to History, the 2003-2006 retrospective of the Smithsonian Museum, relevant collective or individual responses to the 2001 attacks took the form of a catalogue, a list, a vertical or horizontal juxtaposition of data, objects, and memories, evoking a suggestive parallel to the organizing principle of past relics collected in museums and garbage stratified in sanitary landfills.

  13. Detection of Fumonisins in Fresh and Dehydrated Commercial Garlic.

    Science.gov (United States)

    Tonti, Stefano; Mandrioli, Mara; Nipoti, Paola; Pisi, Annamaria; Toschi, Tullia Gallina; Prodi, Antonio

    2017-08-16

    An epidemic fungal disease caused by Fusarium proliferatum, responsible for fumonisin production (FB1, FB2, and FB3), has been reported in the main garlic-producing countries in recent years. Fumonisins are a group of structurally related toxic metabolites produced by this pathogen. The aim of this work was to establish an enzyme-linked immunosorbent assay (ELISA) procedure, mostly applied to cereals, that is suitable for fumonisin detection in garlic and compare these results to those obtained by high-performance liquid chromatography (HPLC) and screening of fresh and dehydrated garlic for toxicological risk. The results show good correlation between the two analytical methods. In fresh symptomatic garlic, fumonisin levels were higher in the basal plates than those in the portions with necrotic spots. Among the 56 commercially dehydrated garlic samples screened, three were positive by ELISA test and only one was above the limit of quantitation. The same samples analyzed by HPLC showed the presence of FB1 in trace amounts that was below the limit of quantitation; FB2 and FB3 were absent. The results are reassuring, because no substantial contamination by fumonisins was found in commercial garlic.

  14. Microbiology of organic and conventionally grown fresh produce.

    Science.gov (United States)

    Maffei, Daniele F; Batalha, Erika Y; Landgraf, Mariza; Schaffner, Donald W; Franco, Bernadette D G M

    2016-12-01

    Fresh produce is a generalized term for a group of farm-produced crops, including fruits and vegetables. Organic agriculture has been on the rise and attracting the attention of the food production sector, since it uses eco-agricultural principles that are ostensibly environmentally-friendly and provides products potentially free from the residues of agrochemicals. Organic farming practices such as the use of animal manure can however increase the risk of contamination by enteric pathogenic microorganisms and may consequently pose health risks. A number of scientific studies conducted in different countries have compared the microbiological quality of produce samples from organic and conventional production and results are contradictory. While some have reported greater microbial counts in fresh produce from organic production, other studies do not. This manuscript provides a brief review of the current knowledge and summarizes data on the occurrence of pathogenic microorganisms in vegetables from organic production. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  15. Ensuring Microbiological Safety of Fresh Eggs by Using Gamma Irradiation

    International Nuclear Information System (INIS)

    Hammad, A. A.; Swailam, H.M.; Aly, W. F.

    2008-01-01

    Egg shell sanitizing practices are necessary to improve microbiological safety of fresh eggs. In this work, fifteen fresh eggs samples of each (Balady, White and Brown eggs) were collected from different local supermarkets to evaluate their microbiological quality. The results indicated that Balady eggs were higher in microbial population in comparison with the two other varieties. Salmonella spp. was detected in 9 (60%) samples of Balady eggs. The effect of gamma irradiation at dose of 0, 2, 3 and 4 kGy on microbiological quality of Balady eggs during storage at 4±1 degree C for 35 days was studied. The effects of irradiation and storage on total cholesterol, and total carotenoids contents in Balady eggs were also investigated. The results showed that irradiation at 3 kGy reduced total aerobic bacterial counts. Coliform bacteria, E. coli and Salmonella spp. were completely eliminated. Total cholesterol and total carotenoids were decreased in yolk egg as a result of irradiation. Treated Balady eggs with irradiation dose of 3 kGy were efficient and sufficient for ensuring safety, maintain quality and extend shelf-life.

  16. Effects of Eating Fresh Lean Pork on Cardiometabolic Health Parameters

    Directory of Open Access Journals (Sweden)

    Peter R. C. Howe

    2012-07-01

    Full Text Available High protein meat-based diets are commonly promoted for weight loss, supposedly by increasing satiety and energy expenditure. Pork is a good source of protein however little information on the metabolic effects of pork consumption exists. This pilot study aimed to examine whether regular consumption of fresh lean pork could improve body composition and cardiovascular risk factors in a 6 month parallel intervention trial. 164 overweight adults (mean BMI 32 were randomly assigned to incorporate up to 1 kg pork/week by substituting for other foods or maintain their habitual diet (control. Plasma levels of lipids, glucose and insulin, BMI, waist/hip circumference, blood pressure, heart rate and arterial compliance were measured at baseline and 3 and 6 months. Body composition was determined using dual energy X-ray absorptiometry. A total of 144 volunteers completed and volunteers in the pork group increased their intake 10 fold by substituting pork for mainly beef and chicken. After 3 months, there were significant (p ≤ 0.01 reductions in weight, BMI, waist circumference, % body fat, fat mass and abdominal fat in the pork group relative to controls, which persisted for 6 months. There was no change in lean mass, indicating that the reduction in weight was due to loss of fat mass. There were no significant effects on other metabolic parameters. Regular consumption of lean fresh pork may improve body composition.

  17. Greenhouse heating with a fresh water floating collector solar pond

    International Nuclear Information System (INIS)

    Arbel, A.; Sokolov, M.

    1991-01-01

    The fresh water floating collector solar pond was investigated both experimentally and theoretically in a previous work, and it is now matched, by simulation, with the heat load requirements of a greenhouse. Results of the simulation indicate that such a pond is a potential energy source for greenhouse heating. This is especially true when the material properties are such that solar absorption and storage are enhanced. This paper reports that to demonstrate this point, three sets of collectors constructed with materials of different physical (radiation) properties were tested. One set is constructed of common materials which are readily available and are normally used as covers for greenhouses. The second set made of improved materials which are also available but have a smaller long-wave transmittance. The last set made of ideal material which additionally possesses selective radiation absorption properties. Collectors made of ideal materials make a superior solar pond; thus, manufacturing films with improved properties should become a worthwhile challenge for the agricultural polyethylene-films industry. Preliminary economic studies indicate that even with the low oil (<$20/Bbl) prices which exist between 1986-1989, the fresh water floating collectors solar pond provides an economically attractive alternative to the conventional oil-burning heating system. This is especially true in mild climate areas and when the large initial investment is justified by long-term greenhouse utilization planning

  18. Dimensional measurement of fresh fuel bundle for CANDU reactor

    International Nuclear Information System (INIS)

    Jo, Chang Keun; Cho, Moon Sung; Suk, Ho Chun; Koo, Dae Seo; Jun, Ji Su; Jung, Jong Yeob

    2005-01-01

    This report describes the results of the dimensional measurement of fresh fuel bundles for the CANDU reactor in order to estimate the integrity of fuel bundle in two-phase flow in the CANDU-6 fuel channel. The dimensional measurements of fuel bundles are performed by using the 'CANDU Fuel In-Bay Inspection and Dimensional Measurement System', which was developed by this project. The dimensional measurements are done from February 2004 to March 2004 in the CANDU fuel storage of KNFC for the 36 fresh fuel bundles, which are produced by KNFC and are waiting for the delivery to the Wolsong-3 plant. The detail items of dimensional measurements are included fuel rod and bearing pad profiles of the outer ring in fuel bundle, diameter of fuel bundle, bowing of fuel bundle, fuel rod length, and surface profile of end plate profile. The measurement data will be compared with those of the post-irradiated bundles cooled in Wolsong-3 NPP spent fuel pool by using the same bundles and In-Bay Measurement System. So, this analysis of data will be applied for the evaluation of fuel bundle integrity in two-phase flow of the CANDU-6 fuel channel

  19. Determination of Phytoestrogen Content in Fresh-Cut Legume Forage

    Directory of Open Access Journals (Sweden)

    Pavlína Hloucalová

    2016-07-01

    Full Text Available The aim of the study was to determine phytoestrogen content in fresh-cut legume forage. This issue has been much discussed in recent years in connection with the health and safety of feedstuffs and thus livestock health. The experiments were carried out on two experimental plots at Troubsko and Vatín, Czech Republic during June and July in 2015. Samples were collected of the four forage legume species perennial red clover (variety “Amos”, alfalfa (variety “Holyně”, and annuals Persian clover and Alexandrian clover. Forage was sampled twice at regular three to four day intervals leading up to harvest and a third time on the day of harvest. Fresh and wilted material was analyzed using liquid chromatography–mass spectrometry (LC-MS. Higher levels ( p < 0.05 of isoflavones biochanin A (3.697 mg·g −1 of dry weight and formononetin (4.315 mg·g −1 of dry weight were found in red clover than in other species. The highest isoflavone content was detected in red clover, reaching 1.001% of dry matter ( p < 0.05, representing a risk for occurrence of reproduction problems and inhibited secretion of animal estrogen. The phytoestrogen content was particularly increased in wilted forage. Significant isoflavone reduction was observed over three to four day intervals leading up to harvest.

  20. The bioconcentration of 131I in fresh water fish

    International Nuclear Information System (INIS)

    Yu, K.N.; Cheung, T.; Young, E.C.M.; Luo, D.L.

    1996-01-01

    The dynamic characteristics of the radionuclide concentration process in fresh water fish have been studied. The experimental data for the tilapias were fitted using a simple compartment model to get characteristics parameters such as concentration factors, elimination rate constants, and initial concentration rates, which are 3.08 Bq kg -1 /Bq L -1 , 0.00573 h -1 , and 12.42 Bq kg -1 h -1 , respectively. The relative concentrations of 131 I in different parts, i.e., head, gills, flesh, bone and internal organs, of the tilapias are also determined, which are found to be 10.8, 15.4, 26.1, 11.0, and 37.0%, respectively. The effects of different factors on the transfer of radionuclides in fresh water fishes are also discussed. Experiments on the tilapias and the common carp show that the variation of concentration factors for different species may be significant even for the same radionuclide and the same ecological system. On the other hand, the variation in the concentration factors for the flesh of the tilapias is not significant for a certain range of 131 I concentrations in the water. 12 refs., 1 fig., 1 tab

  1. Gamma ray-irradiation in fresh allo-joint transplantation

    International Nuclear Information System (INIS)

    Watanabe, Hiroshi

    1995-01-01

    In the first of a series of experiments in rat designed to assess the efficacy of gamma ray irradiation in fresh allo-joint transplantation, it was found that the optimal gamma ray dosage was 4 Gy. At this dosage level, the irradiation rays suppressed the viability of marrow cells which had the highest antigenicity, with no injury to the bone or articular cartilage. In a second experiment, a fresh homologous knee joint was irradiated at 4 Gy and then transplanted while administering the donor's splenic cell suspension (for specific immunosuppression) and the immunosuppressive agent cyclosporine (5 mg/kg) to the recipient rat. All the rats that received a pre-irradiated knee joint graft survived until sacrificed for evaluation without showing any sign of host rejection. In these rats, bone fusion had occurred between the host bone and the graft by the 8th postoperative week. Degeneration of the articular cartilage was similar between the rats that had received a pre-irradiated graft and those that had not. These findings indicated that 4 Gy gamma ray irradiation to a graft before transplantation provided an effective means of immunosuppression. (author)

  2. Limnological study of fresh water of rawal dam, islamabad

    International Nuclear Information System (INIS)

    Leghari, M.K.; Ghafar, S.A.

    2005-01-01

    The limnological study of Fresh water of Rawal Dam was carried out for the first time from June 2000 to May 2001 during Research Project. C-PMNHIBio(311). Data was collected on physico-chemical properties of the Fresh water and the Algal Flora. The fluctuations of temperature, pH, Dissolved oxygen, salinity, T.D.S., Total hardness etc., were studied. From algal flora, a total. 291 algal species were found to belong to 108 genera 10 Phylum. From Cyanophyta, 68 species belongs to 21 genera with 23.37%, Volvocophyta 107 species belongs to 40 genera with 36.77%, Chlorophyta 18 species belongs to 13 genera with 6.19%, Bacillariophyta 66 species 19 genera with 22.67%, Chrysophyta 3 species belongs to 2 genera with 1.03%, Xanthophyta 5 species belongs to 5 genera with 1.72%, Vaucheriophyta 1 species belongs to 1 genus with 0.34%, Euglenophyta 16 species belongs to 4 genera with 1.72%, Dinophyta 5 species belongs to 2 genera with 1.72%, Charophyta 2 species belong to 1 genus with 0.7%, were recorded and classified by latest classification according to Shameel (2001). The present work shows the changes in physico-chemical factor along with algal flora in Rawal Dam during collection period and co-relation of different factors. (author)

  3. Keeping education fresh-not just in microbiology.

    Science.gov (United States)

    Fahnert, Beatrix

    2017-11-15

    Innovative practice from around the globe, addressing a range of recent educational themes and trends, was published in the FEMS Microbiology Letters virtual Thematic Issue 'Keeping Education Fresh' in October 2017. Its thought-provoking content is reviewed here to more directly facilitate reflections and discussions in the professional community. The focus is on best practice approaches when enhancing student engagement, how to adjust those to the diversity of learners, learning situations and infrastructures, and to a broad range of subjects. The need for authentic learning and to move away from didactic teaching is emphasized. The 'students as researchers' theme is featured e.g. in context of service learning. Creative approaches are presented such as using performing arts, popular culture and gamification. The development of interdisciplinary and intercultural competences, and the exploration of socioscientific themes and philosophical issues are considered. Revisions of curricula and programmes, reflective of educational advancements and sector drivers, are discussed from undergraduate to postgraduate and professional specialist level also in light of problem-based learning, interactive distance and on-campus learning, and even the legacy of Massive Open Online Courses. Such changes always require resources and skills, and carry risks. Yet, innovation is a risk worth taking to keep education fresh. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  4. Shallow and deep fresh impact craters in Hesperia Planum, Mars

    Science.gov (United States)

    Mouginis-Mark, Peter J.; Hayashi, Joan N.

    1993-01-01

    The depths of 109 impact craters about 2-16 km in diameter, located on the ridged plains materials of Hesperia Planum, Mars, have been measured from their shadow lengths using digital Viking Orbiter images (orbit numbers 417S-419S) and the PICS computer software. On the basis of their pristine morphology (very fresh lobate ejecta blankets, well preserved rim crests, and lack of superposed impact craters), 57 of these craters have been selected for detailed analysis of their spatial distribution and geometry. We find that south of 30 deg S, craters less than 6.0 km in diameter are markedly shallower than similar-sized craters equatorward of this latitude. No comparable relationship is observed for morphologically fresh craters greater than 6.0 km diameter. We also find that two populations exist for older craters less than 6.0 km diameter. When craters that lack ejecta blankets are grouped on the basis of depth/diameter ratio, the deeper craters also typically lie equatorward of 30 S. We interpret the spatial variation in crater depth/diameter ratios as most likely due to a poleward increase in volatiles within the top 400 m of the surface at the times these craters were formed.

  5. Prediction of egg freshness during storage using electronic nose.

    Science.gov (United States)

    Yimenu, Samuel M; Kim, J Y; Kim, B S

    2017-10-01

    The aim of the present study was to investigate the potential of a fast gas chromatography (GC) e-nose for freshness discrimination and for prediction of storage time as well as sensory and internal quality changes during storage of hen eggs. All samples were obtained from the same egg production farm and stored at 20 °C for 20 d. Egg sampling was conducted every 0, 3, 6, 9, 12, 16, and 20 d. During each sampling time, 4 egg cartons (each containing 10 eggs) were randomly selected: one carton for Haugh units, one carton for sensory evaluation and 2 cartons for the e-nose experiment. The e-nose study included 2 independent test sets; calibration (35 samples) and validation (28 samples). Every sampling time, 5 replicates were prepared from one egg carton for calibration samples and 4 replicates were prepared from the remaining egg carton for validation samples. Sensors (peaks) were selected prior to multivariate chemometric analysis; qualitative sensors for principal component analysis (PCA) and discriminant factor analysis (DFA) and quantitative sensors for partial least square (PLS) modeling. PCA and DFA confirmed the difference in volatile profiles of egg samples from 7 different storage times accounting for a total variance of 95.7% and 93.71%, respectively. Models for predicting storage time, Haugh units, odor score, and overall acceptability score from e-nose data were developed using calibration samples by PLS regression. The results showed that these quality indices were well predicted from the e- nose signals, with correlation coefficients of R2 = 0.9441, R2 = 0.9511, R2 = 0.9725, and R2 = 0.9530 and with training errors of 0.887, 1.24, 0.626, and 0.629, respectively. As a result of ANOVA, most of the PLS model results were not significantly (P > 0.05) different from the corresponding reference values. These results proved that the fast GC electronic nose has the potential to assess egg freshness and feasibility to predict multiple egg freshness indices

  6. Effect of gamma-irradiation on flavor compounds of fresh mushrooms

    International Nuclear Information System (INIS)

    Mau, J.L.; Hwang, S.J.

    1997-01-01

    Fresh mushrooms (Agaricus bisporus) were gamma-irradiated with doses of 1,2, and 5 kGy. The volatile compounds were isolated using a Lickens-Nickerson apparatus and analyzed using gas chromatography and gas chromatography-mass spectrometry. The amount of total volatiles was greatly affected by the doses applied. The amounts of benzaldehyde and benzyl alcohol were not affected by gamma-irradiation and ranged from 8.94 to 11.79 and from 0.696 to 1.503 micrograms/g, respectively. The amounts of eight-carbon compounds decreased as the doses of gamma-irradiation increased, from 41.73 for the control (0 kGy) to 20.06 (1 kGy), 8.77 (2 kGy), and 4.04 micrograms/g (5 kGy irradiated mushrooms). The major eight-carbon compound was 1-octen-3-ol, and its amount decreased from 30.34 (the control) to 14.18 (1 kGy), 6.22 (2 kGy), and 2.92 micrograms/g (5 kGy)

  7. A method for establishing human primary gastric epithelial cell culture from fresh surgical gastric tissues.

    Science.gov (United States)

    Aziz, Faisal; Yang, Xuesong; Wen, Qingping; Yan, Qiu

    2015-08-01

    At present, biopsy specimens, cancer cell lines and tissues obtained by gastric surgery are used in the study and analysis of gastric cancer, including the molecular mechanisms and proteomics. However, fibroblasts and other tissue components may interfere with these techniques. Therefore, the present study aimed to develop a procedure for the isolation of viable human gastric epithelial cells from gastric surgical tissues. A method was developed to culture human gastric epithelial cells using fresh, surgically excised tissues and was evaluated using immunocytochemistry, periodic acid-Schiff (PAS) staining and cell viability assays. Low cell growth was observed surrounding the gastric tissue on the seventh day of tissue explant culture. Cell growth subsequently increased, and at 12 days post-explant a high number of pure epithelial cells were detected. The gastric cancer cells exhibited rapid growth with a doubling time of 13-52 h, as compared to normal cells, which had a doubling time of 20-53 h. Immunocytochemical analyses of primary gastric cells revealed positive staining for cytokeratin 18 and 19, which indicated that the culture was comprised of pure epithelial cells and contained no fibroblasts. Furthermore, PAS staining demonstrated that the cultured gastric cells produced neutral mucin. Granulin and carbohydrate antigen 724 staining confirmed the purity of gastric cancer and normal cells in culture. This method of cell culture indicated that the gastric cells in primary culture consisted of mucin-secreting gastric epithelial cells, which may be useful for the study of gastric infection with Helicobacter pylori and gastric cancer.

  8. Molecular diagnosis of rhino-orbito-cerebral mucormycosis from fresh tissue samples.

    Science.gov (United States)

    Zaman, Kamran; Rudramurthy, Shivaprakash Mandya; Das, Ashim; Panda, Naresh; Honnavar, Prasanna; Kaur, Harsimran; Chakrabarti, Arunaloke

    2017-08-01

    We aimed to evaluate a PCR-based technique for the diagnosis of mucormycosis and the identification of fungi from fresh tissue specimens in patients with rhino-orbito-cerebral-mucormycosis (ROCM). Fifty cases of ROCM were included in the study. Conventional identification was performed using microscopy and culture. Molecular diagnosis was performed by amplifying the ribosomal DNA using pan-fungal ITS primers and semi-nested Mucorales-specific primers of the 18S region. The amplified products were sequenced to identify the agents. The utility of PCR-RFLP of the 18S region of rDNA was evaluated to identify the Mucorales. The ROCM cases were diagnosed by the demonstration of aseptate ribbon-like hyphae in biopsy specimens collected from the patients. Isolation was possible in 24 (48 %) samples. The ITS2 PCR confirmed mucormycosis in 27 cases (54 %; CI 59.4-68.2). By comparison, Mucorales-specific PCR was able to amplify DNA and the sequence enabled the identification of Mucorales speciesin all the patients. PCR-RFLP of the 18S region of rDNA could only identify the agent to genus level. The molecular technique was able to identify Mucorales species in 26 (42 %) cases that were negative by culture. Mucorales-specific semi-nested PCR targeting the 18S region is a better technique than ITS2 PCR for diagnosis. PCR-RFLP of the 18S region helps in identification to genus level.

  9. Management of Meloidogyne incognita on tomato with endophytic bacteria and fresh residue of Wasabia japonica.

    Science.gov (United States)

    Li, G J; Dong, Q E; Ma, L; Huang, Y; Zhu, M L; Ji, Y P; Wang, Q H; Mo, M H; Zhang, K Q

    2014-10-01

    To characterize the nematicidal endophytic bacteria (NEB) of Wasabia japonica (wasabi) and evaluated the control efficacies of promising NEB as well as fresh wasabi residue (FWR) against Meloidogyne incognita on tomato. By in vitro bioassay, 53 NEB strains showing nematicidal efficacies of >50% against J2 of M. incognita were isolated from wasabi. Basing on 16S rRNA gene sequences, these NEB were identified into 18 species of 11 genera. In greenhouse, incorporation of selected NEB culture or FWR into potted soil significantly reduced infection of M. incognita on tomato. Treating tomatoes with either FWR or NEB of Raoultella terrigena RN16 and Pseudomonas reinekei SN21 in the field yielded excellent control efficacies against M. incognita, especially the combinations of FWR with either R. terrigena RN16 or Ps. reinekei SN21 at doses of 50 g plus 100 ml per plant or more. The results established that R. terrigena RN16 and Ps. reinekei SN21 applied separately or combined with FWR have the potential to provide bioprotection agents against M. incognita. This study provides novel way for disease management using combination of endophyte and host residue. © 2014 The Society for Applied Microbiology.

  10. Drop testing of the Westinghouse fresh nuclear fuel package

    International Nuclear Information System (INIS)

    Shappert, L.B.; Sanders, C.F.

    1993-01-01

    The Westinghouse Columbia Fuel Fabrication Facility has decided to develop and certify a new fresh fuel package design (type A, fissile) that has the capability to transport more highly enriched fuel than was previously possible. A prototype package was tested in support of the Safety Analysis Report of the Packaging (SARP). This paper provides detailed information on the tests and test results. A first prototype test was carried out at the STF, and the design did not give the safety margin that Westinghouse wanted for their containers. The data from the test were used to redesign the connection between the clamping frame and the pressure pad, and the tests were reinitiated. Three packages were then tested at the STF. All packages met the acceptance criteria and acceleration information was obtained that provided an indication of the behavior of the cradle and strongback which holds the fuel assemblies and nuclear poison in place. (J.P.N.)

  11. Low hygroscopicity of ambient fresh carbonaceous aerosols from pyrotechnics smoke

    Science.gov (United States)

    Carrico, Christian M.; Gomez, Samantha L.; Dubey, Manvendra K.; Aiken, Allison C.

    2018-04-01

    Pyrotechnics (fireworks) displays are common for many cultures worldwide, with Independence Day celebrations occurring annually on July 4th as the most notable in the U.S. Given an episodic nature, fireworks aerosol properties are poorly characterized. Here we report observations of optical properties of fresh smoke emissions from Independence Day fireworks smoke sampled at Los Alamos National Laboratory, New Mexico U.S.A. on 4-5 July 2016. Aerosol optical properties were measured with a photoacoustic extinctiometer (PAX, DMT, Inc., Model 870 nm) at low RH laboratory testing with ground-level sparklers showed that pyrotechnics smoke can generate a strong hygroscopic response, however. As confirmed with chemical analysis, the chemistry of the fireworks was key to defining the hygroscopic response. Sparkler smoke was dominated by salt species such as hygroscopic potassium chloride while it lacked the black powder explosives in aerial fireworks that contribute organic and elemental carbon to its non-hygroscopic smoke.

  12. Relating Fresh Concrete Viscosity Measurements from Different Rheometers.

    Science.gov (United States)

    Ferraris, Chiara F; Martys, Nicos S

    2003-01-01

    Concrete rheological properties need to be properly measured and predicted in order to characterize the workability of fresh concrete, including special concretes such as self-consolidating concrete (SCC). It was shown by a round-robin test held in 2000 [1,2] that different rheometer designs gave different values of viscosity for the same concrete. While empirical correlation between different rheometers was possible, for a procedure that is supposed to "scientifically" improve on the empirical slump tests, this situation is unsatisfactory. To remedy this situation, a new interpretation of the data was developed. In this paper, it is shown that all instruments tested could be directly and quantitatively compared in terms of relative plastic viscosity instead of the plastic viscosity alone. This should eventually allow the measurements from various rheometer designs to be directly calibrated against known standards of plastic viscosity, putting concrete rheometry and concrete workability on a sounder materials science basis.

  13. Chemical dispersibility testing of fresh and weathered oils

    International Nuclear Information System (INIS)

    Brandvik, P.J.; Daling, P.S.; Aareskjold, K.

    1991-05-01

    This activity in the DIspersants on Weathered Oils-project (DIWO) is a continuation and an extended study of the dispersibility study described in DIWO report No. 3. The main objective has been to study the chemical dispersibility of fresh and weathered oils produced or transported in Norwegian waters. Other important aims of this study have been: To correlate the effectiveness results obtained by three different laboratory methods; to determine the relationship between the dispersant effectiveness and the change in the oils' physico-chemical properties due to weathering (topping, photo-oxidation and w/o-emulsification). This study has been performed with 8 different oil types and 12 different weathering degrees of each oil type. The work performed clearly demonstrates that the oil type and especially the weathering properties are essential for the performance of dispersants at sea. 41 figs., 5 tabs., 16 refs

  14. Research of radiation technology in keeping pet feed fresh

    International Nuclear Information System (INIS)

    Lin Qin; Zhang Tongcheng; Liu Qingfang; Wang Chunlei

    2002-01-01

    Objective: To find a effective, simple, quick method of radiation keeping fresh technology. Method: To detect the number of bacterium colony and pathogen and D 10 in Raschide dog chews firstly, then calculate them with the formulate of S D = D 10 log N 0 /SAL. Result: The total number of bacterium colony and pathogen in Raschide dog chews, a kind of pet feed, were detected. The mean total number of colony forming units was 3980 every gram and G + bacilli predominant, cocci less. The D 10 value of G + bacilli was 1.51 KGy. Conclusion: According to the certain formula method, the product SAL can be kept at 10 -6 level with 14.50 KGy radiation dose

  15. Technology Optimization of Lysozyme's Fresh Maintaining Effect on Apple.

    Science.gov (United States)

    Jun-Hong, Liu; Kun-Yu, Wang

    2016-10-03

    Lysozyme is a kind of alkaline globin, which functions well in the degradation of the cell wall of microbe. Currently, lysozyme is widely used in various fields, such as medicine, fruit, and vegetable industry, dairy industry, and so on. Therefore, the exploitation and utilization of lysozyme is of significant economic benefit. Taking apple as material, weight loss ratio and reducing sugar content as objectives, this paper studied the fresh-keeping effect of lysozyme. Influential factors, lysozyme concentration, soaking time, modified temperature, and reaction time were discussed in detail. The results showed that reducing sugar content was 2.043% and the weight loss ratio was the minimum in the presence of soaking time of 2 min, modified temperature of 65 °C, reaction time of 4 d, and lysozyme concentration of 0.5 g/L. © 2016 Institute of Food Technologists®.

  16. Freshly squeezed: anaphylaxis caused by drone larvae juice.

    Science.gov (United States)

    Stoevesandt, J; Trautmann, A

    2017-11-30

    Drone larvae are mostly considered a by-product of beekeeping, but have recently been advo-cated as a high-protein source of food. There are as yet no data concerning their allergenic po-tential. We report on a 29-year old bee keeper who experienced an anaphylactic reaction following the consumption of a freshly prepared beverage from raw drone larvae. Larvae-specific sensitization was confirmed by prick-to-prick and basophil activation testing. Bee stings and classical bee products including honey and royal jelly were tolerated. This is the hitherto first report on IgE-mediated allergy to drone larvae. We suggest that a certain awareness towards the allergenicity of bee larvae is required.

  17. Successful selling of packed fresh fruit and vegetable

    Directory of Open Access Journals (Sweden)

    Radmila Presová

    2009-01-01

    Full Text Available The paper deals with the marketability of packaged fruit and vegetables, as a new trend of satisfying consumer needs, in connection with selling-culture of stall selling and self-stores sales. It emphasize fact that food have to be wrapped into the quality, health packages, under the conditions of the Act. 477/2001 Coll.Specific data for the analytical part of this paper was provided by Hortim International Ltd. This company has operate on the Czech market since 1995. Analysis of the economic results confirmes the legitimacy of the sale of packaged fresh fruits and vegetables, but there will be neccesary longer lasting promotion. This fact also confirmed the research of consumer demand, that was made by authors. Determinant for the success sales are these factors: consumer confidence that the packaged products of fruit and vegetables are high quality.

  18. Development of process control for the irradiation of fresh mangoes

    Energy Technology Data Exchange (ETDEWEB)

    Cabalfin, E G; Lanuza, L G; Maningas, A L; Solomon, H M; Madera, G G; Pares, F A [Philippine Nuclear Research Institute, Quezon City (Philippines)

    2001-03-01

    Dose distribution studies in mangoes contained in boxes used in commercial trade for export, were done using the multipurpose irradiation facility at the Philippine Nuclear Research Institute. The mangoes were irradiated at a target dose of 100 Gy, the dose required for quarantine treatment of fresh mangoes against fruitflies. Positions of minimum dose and maximum dose were identified and dose uniformity ratio was determined. Fricke and Gammachrome YR dosimeters were used for the dose distribution studies. The performance of STERIN threshold indicators was evaluated by irradiating them at different doses. STERIN 125 indicators were also attached to the surface of the mango boxes during the dose distribution studies. STERIN indicators can be useful to differentiate between irradiated and unirradiated products. (author)

  19. Development of process control for the irradiation of fresh mangoes

    International Nuclear Information System (INIS)

    Cabalfin, E.G.; Lanuza, L.G.; Maningas, A.L.; Solomon, H.M.; Madera, G.G.; Pares, F.A.

    2001-01-01

    Dose distribution studies in mangoes contained in boxes used in commercial trade for export, were done using the multipurpose irradiation facility at the Philippine Nuclear Research Institute. The mangoes were irradiated at a target dose of 100 Gy, the dose required for quarantine treatment of fresh mangoes against fruitflies. Positions of minimum dose and maximum dose were identified and dose uniformity ratio was determined. Fricke and Gammachrome YR dosimeters were used for the dose distribution studies. The performance of STERIN threshold indicators was evaluated by irradiating them at different doses. STERIN 125 indicators were also attached to the surface of the mango boxes during the dose distribution studies. STERIN indicators can be useful to differentiate between irradiated and unirradiated products. (author)

  20. Update on Fresh Fuel Characterization of U-Mo Alloys

    International Nuclear Information System (INIS)

    Burkes, D.E.; Wachs, D.M.; Keiser, D.D.; Okuniewski, M.A.; Jue, J.F.; Rice, F.J.; Prabhakaran, R.

    2009-01-01

    The need to provide more accurate property information on U-Mo fuel alloys to operators, modellers, researchers, fabricators, and government increases as success of the GTRI Reactor Convert program continues. This presentation provides an update on fresh fuel characterization activities that have occurred at the INL since the RERTR 2008 conference in Washington, D.C. The update is particularly focused on properties recently obtained and on the development progress of new measurement techniques. Furthermore, areas where useful and necessary information is still lacking is discussed. The update deals with mechanical, physical, and microstructural properties for both integrated and separate effects. Appropriate discussion of fabrication characteristics, impurities, thermodynamic response, and effects on the topic areas are provided, along with a background on the characterization techniques used and developed to obtain the information. Efforts to measure similar characteristics on irradiated fuel plates are discussed.

  1. Dispersant effectiveness in the field on fresh oils and emulsions

    International Nuclear Information System (INIS)

    Lunel, T.; Davies, L.

    1996-01-01

    A detailed data set on the effectiveness of dispersants on fresh oils and emulsions, was presented. The data set could be used to calibrate laboratory dispersant tests and dispersion models so that oil spill response teams would have accurate information to make decisions regarding remediation processes. AEA Technology developed steady state continuous release experiments to provide a data set with quantitative measures of dispersant effectiveness in the field. The Sea Empress incident was closely monitored in order to compare the quantification obtained through field trials. It was noted that the prediction of the percentage of oil dispersed chemically is not the only indication of whether or not to use a dispersant. The important determinant to consider should be the extent to which the natural dispersion process would be enhanced by dispersant application. 17 refs., 5 tabs., 18 figs

  2. Development of fresh fuel packaging for ATR demonstration reactor

    International Nuclear Information System (INIS)

    Kurakami, J.; Kurita, I.

    1993-01-01

    Related to development of the demonstration advanced thermal reactor, it is necessary and important to develop transport packaging which is used for transporting fresh fuel assemblies. Therefore, the packaging is now being developed in Power Reactor and Nuclear Fuel Development Corporation (PNC). Currently, PNC is fabricating two prototype packagings based on the final design, and land cruising and vibration tests, handling performance tests and prototype packaging tests will be executed with prototype packagings in order to experimentally confirm the soundness of packaging and its contents and the propriety of design technique. This paper describes the summary of general specifications and structures of this packaging and the summary of preliminary safety analysis of package. (J.P.N.)

  3. On-site detection of packaged squid freshness

    Science.gov (United States)

    Ahmad, Noor Azizah; Heng, Lee Yook; Salam, Faridah; Hanifah, Sharina Abu

    2018-04-01

    The development of indicator label for detection of total volatile basic nitrogen (TVB-N) is described. Dye extract from edible plants containing anthocyanins was immobilized onto iota-carrageenan as polymer matrix. TVB-N detection worked based on pH increase as the basic deterioration volatile amines generated in the package headspace. Results showed that the indicator label has changed color from blue to green after 12 hours of storage at ambient conditions. The TVB-N value was 38.9648 mg /100 g which is exceeded of acceptability level for seafood products. The pH value of squid flesh has also increased during storage. The colour values of L * and a * negative increases while b* negative decrease with increasing storage time. The indicator label is potentially used as freshness indicator for squid at ambient conditions.

  4. Adhesive and invasive capacities of Edwarsiella tarda isolated from South American sea lion

    OpenAIRE

    Fernández, Araceli; Paz Villanueva, María; González, Mario; Fernández, Fabiola; Latif, Fadua; Flores, Sandra Nonier; Fernández, Heriberto

    2014-01-01

    Edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. Although E. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. A survey of zoonotic intestinal bacteria in fresh feces from South American sea lions (SASL) Otaria flavescens, reported E. tarda as the most frequently isolated species. In this study, we used HEp-2 cells to establish in vitro the adherence and inv...

  5. A rapid supercritical fluid extraction method for the qualitative detection of 2-alkylcyclobutanones in gamma-irradiated fresh and sea water fish

    International Nuclear Information System (INIS)

    Tewfik, I.H.; Ismail, H.M.; Sumar, S.

    1999-01-01

    2-Alkylcyclobutanones are routinely used as chemical markers for irradiated foods containing lipids. However, current extraction procedures (soxhlet-Florisil chromatography) for the isolation of these markers involve a long and tedious clean-up regime prior to GC-MS identification. A simple and rapid method for the isolation of these markers using carbon dioxide as a super critical fluid is described for low lipid content fish samples (fresh and sea water) irradiated up to 8kGy. The presence of 2-dodecylcyclobutanone (2-DCB), a radiolytic marker, was confirmed in all irradiated fish samples at all doses. This was a clear indication that the fish samples had been irradiated and that both methods of isolation (florisil and supercritical fluid extraction) were capable of qualitatively extracting this marker. Supercritical fluid extraction is proposed as an alternative extraction procedure to the florisil chromatography method currently in use and has the added advantage of a considerably shorter extraction time

  6. Viability Tests for Fresh and Stored Haemopoietic Cells

    Energy Technology Data Exchange (ETDEWEB)

    Fliedner, T. M. [Abteilung fuer klinische Physiologie, Zentrum fuer Klinische Grundlagenforschung, Universitaet Ulm, Ulm, Federal Republic of Germany (Germany)

    1969-07-15

    This paper reviews current methods of measurement of the viability of fresh and stored haemopoietic cells. The life expectancy of granulocytes and monocytes after transfusion can be studied by in-vitro labelling with {sup 3}H-DFP and subsequent autoradiography. The evaluation of data in about 30 patients with various haemopoietic conditions indicates a wide variation of the disappearance half-time of granulocytes. {sup 3}H-cytidine labels essentially all lymphocytes in vitro, predominantly in their RNA. Transfusion of {sup 3}H-cytidine-labelled lymphocytes enables one to measure the lower limit of their life-expectancy as well as their rate of RNA metabolism. If bone-marrow cells are labelled in vitro with {sup 3}H-thymidine and subsequently transfused, their capability to circulate, to reach the haemopoietic tissue of the host, to proliferate and to mature can be demonstrated. However, the repopulating capacity of frozen and thawed marrow is independent of the ability of {sup 3}H-TDR-labelled marrow cells to circulate, proliferate and mature. It is assumed that bone-marrow cells capable of repopulating depleted haemopoietic tissue are resting under steady-state conditions and can be labelled by means of {sup 3}H-TDR only using special conditions. Thus the only viability tests for fresh and stored bone-marrow cells at present appear to be bioassay methods at the animal experimental level. The results indicate the need for the development of reliable viability tests for stem cells applicable in both experimental and clinical conditions. (author)

  7. Changes in the business model for Spanish fresh tomato trade

    International Nuclear Information System (INIS)

    Pablo-Valenciano, J. de; Giacinti-Battistuzzi, M.A.; Tassile, T.; García-Azcárate, T.

    2017-01-01

    The business model applied to the fresh tomato trade from Almería is well known in Spain and abroad. The changes in demand are forcing export companies to change their trade strategies to become or remain competitive. The purpose of this paper is to know the business structure and its export dynamics in addition to the changes in trade strategies made by companies in Almería during the 2009-2013 period. The methodology is based on the Herfindahl-Hirshman index and the foreign trade competitiveness index, whereas the congruence analysis is based on Pearson’s correlation coefficients and the RV coefficient. We have also applied the dual multiple factor analysis to verify changes made to the trade policy in leading businesses between two periods or scenarios. The research shows that export-driven companies behave as a moderately concentrated structure and there is an increase in the number of exporters. The competitiveness map for Almería reveals the sector’s trend with regard to the most important markets. We have verified the changes made to their trade strategy to adjust to changes in foreign trade between the 2008/09 and 2012/13 campaigns. Finally, it can be pointed out that, in the analyzed period, changes in the business model for fresh tomato trade of Almería have not been significant, being its structure of moderate concentration. In addition, the great majority of variations in strategies of companies to adapt to the competitive environment have been made by leading companies.

  8. Changes in the business model for Spanish fresh tomato trade

    Energy Technology Data Exchange (ETDEWEB)

    Pablo-Valenciano, J. de; Giacinti-Battistuzzi, M.A.; Tassile, T.; García-Azcárate, T.

    2017-09-01

    The business model applied to the fresh tomato trade from Almería is well known in Spain and abroad. The changes in demand are forcing export companies to change their trade strategies to become or remain competitive. The purpose of this paper is to know the business structure and its export dynamics in addition to the changes in trade strategies made by companies in Almería during the 2009-2013 period. The methodology is based on the Herfindahl-Hirshman index and the foreign trade competitiveness index, whereas the congruence analysis is based on Pearson’s correlation coefficients and the RV coefficient. We have also applied the dual multiple factor analysis to verify changes made to the trade policy in leading businesses between two periods or scenarios. The research shows that export-driven companies behave as a moderately concentrated structure and there is an increase in the number of exporters. The competitiveness map for Almería reveals the sector’s trend with regard to the most important markets. We have verified the changes made to their trade strategy to adjust to changes in foreign trade between the 2008/09 and 2012/13 campaigns. Finally, it can be pointed out that, in the analyzed period, changes in the business model for fresh tomato trade of Almería have not been significant, being its structure of moderate concentration. In addition, the great majority of variations in strategies of companies to adapt to the competitive environment have been made by leading companies.

  9. Development of analytical methods for iodine speciation in fresh water

    International Nuclear Information System (INIS)

    Takaku, Yuichi; Ohtsuka, Yoshihito; Hisamatsu, Shun'ichi

    2007-01-01

    Analytical methods for physicochemical speciation of iodine in fresh water samples were developed to elucidate its behavior in the environment. The methods combined inductively coupled plasma mass spectrometry (ICP-MS) with size exclusion high performance liquid chromatography (SEC) or capillary electrophoresis (CE). Freshwater samples were collected from Lake Towada and rivers surrounding the lake. After filtration through a 0.45 μm pore size membrane filter, iodine in the water samples was pre-concentrated with an ultra-filtration filter which had a cut-off size of 10 kDa. The fraction with molecular size over 10 kDa was concentrated to 100 times in the original water, and then introduced into the SEC-ICP-MS. Molecular size chromatograms of all river and lake water samples showed two peaks for iodine concentrations: 40 kDa and 20 kDa. The method for separately determining two valence states of inorganic iodine, I - and IO 3 - , was also developed using the CE-ICP-MS system and it was successfully applied to the fresh water samples. Analysis results of surface water samples in Lake Towada and rivers surrounding the lake indicated that the chemical form of inorganic iodine in all samples was IO 3 - . Additional lake water samples were collected from Lake O-ike-higashi in the Juni-ko area at Shirakami-Sanchi, which is a UNESCO natural world heritage. The lake has a strong thermocline during all seasons; its bottom layer is in a highly reductive state. Depth profiles of I - and IO 3 - clearly showed that I - was not detected in the surface layer, but it was predominant in the bottom layer, and vice versa for IO 3 - . As this separation method is rapid and sensitive, it will be widely used in the future. (author)

  10. The History of Variable Stars: A Fresh Look

    Science.gov (United States)

    Hatch, R. A.

    2012-06-01

    (Abstract only) For historians of astronomy, variable stars are important for a simple reason - stars change. But good evidence suggests this is a very modern idea. Over the millennia, our species has viewed stars as eternal and unchanging, forever fixed in time and space - indeed, the Celestial Dance was a celebration of order, reason, and stability. But everything changed in the period between Copernicus and Newton. According to tradition, two New Stars announced the birth of the New Science. Blazing across the celestial stage, Tycho's Star (1572) and Kepler's Star (1604) appeared dramatically - and just as unexpectedly - disappeared forever. But variable stars were different. Mira Ceti, the oldest, brightest, and most controversial variable star, was important because it appeared and disappeared again and again. Mira was important because it did not go away. The purpose of this essay is to take a fresh look at the history of variable stars. In re-thinking the traditional narrative, I begin with the first sightings of David Fabricius (1596) and his contemporaries - particularly Hevelius (1662) and Boulliau (1667) - to new traditions that unfolded from Newton and Maupertuis to Herschel (1780) and Pigott (1805). The essay concludes with important 19th-century developments, particularly by Argelander (1838), Pickering (1888), and Lockyer (1890). Across three centuries, variable stars prompted astronomers to re-think all the ways that stars were no longer "fixed." New strategies were needed. Astronomers needed to organize, to make continuous observations, to track changing magnitudes, and to explain stellar phases. Importantly - as Mira suggested from the outset - these challenges called for an army of observers with the discipline of Spartans. But recruiting that army required a strategy, a set of theories with shared expectations. Observation and theory worked hand-in-hand. In presenting new historical evidence from neglected printed sources and unpublished

  11. Traceability and Demand Sensitiveness: Evidences from Italian Fresh Potatoes Consumption

    Directory of Open Access Journals (Sweden)

    Francesco Caracciolo

    2011-02-01

    Full Text Available When a traceability system takes place, either when mandatory or voluntary, many questions arise that need to be addressed and answered. One of the firsts concerns whether it introduces new costs with no gain in efficiency or, on the contrary, the system efficiency increases lowering costs and, as a consequence, market price of the good in hand. Among others, another issue that only rarely is addressed regards the effect of a price change on the final market. The objective of this paper was to simulate the effect on fresh Italian vegetables market of prices change due to a newer traceability procedure, focusing on early potato. Reasons why early potato was the main object of our study will be explained in detail later in the paper. Moreover, this study concerns a demand system estimation that has, as main goal, the measurement of own and cross price elasticities as well as expenditure elasticities. Such estimations are not strictly related with traceability because they measure any change in quantity demanded due to price changes due to any market perturbation. However, since early potato is experiencing a peculiar market and chain change in Italy, our simulation is meant to reason in terms of a “what if” approach, formally simulating the effect of any change in price due to an hypothesis of traceability system involved. In order to estimate a demand system, real household consumption data (3,000 observations of a statistically representative sample of the Italian population of households was taken into account. Relevant measures of market variables were estimated by means of a Linear Almost Ideal Demand System implementing a large set of fresh vegetables: potato split in early and late, cabbage, salad, mushrooms, fruits vegetables, roots, asparagus, onion, tomatoes, peppers, cucumbers, beans, zucchinis and others.

  12. Wettability contrasts between fresh and weathered diesel fuels.

    Science.gov (United States)

    Drake, Stephanie S; O'Carroll, Denis M; Gerhard, Jason I

    2013-01-01

    The remediation of non-aqueous phase liquid (NAPL) contaminated sites is impeded due to subsurface complexities, including wettability. Wettability quantifies which of two immiscible fluids preferentially coats a solid. At most contaminated sites water-wetting conditions are typically assumed despite mounting evidence that this is not always the case. In this study, wettability was examined for two NAPL samples of contrasting origin: a fresh and a field sample. Wettability was assessed through (i) cyclical, 'cumulative elapsed contact time' intrinsic contact angle measurements, (ii) interface jar tests, and (iii) cyclical, pseudo-static capillary pressure-saturation curves. The work as a whole demonstrated that while the fresh diesel sample was consistently water-wet, the field diesel sample exhibited repeatable cycles of wettability reversal between water drainage and imbibition. And while wettability hysteresis increased with contact time for the field diesel, the occurrence of wettability reversal at each change of saturation direction was independent of contact time. Such behavior is not easily assessed by standard wettability indices. Moreover, it contrasts with the permanent wettability alteration observed for complex organics (e.g., coal tar) observed in most studies. It is hypothesized that the cyclical wettability reversal is related to cyclical changes in intermediate pore wettability due to sorption of surface active compounds (causing NAPL-wetting imbibition) and rupturing of the soil grain water film (causing water-wet drainage). The wettability differences between the two NAPLs may be due to additives (i.e., a surfactant) in the original formulation and/or byproducts from subsurface weathering. These results support better characterization of site-specific wettability, improved model development and more realistic site conceptual models for improved remediation efforts. Copyright © 2012 Elsevier B.V. All rights reserved.

  13. Freshness indices of roasted coffee: monitoring the loss of freshness for single serve capsules and roasted whole beans in different packaging.

    Science.gov (United States)

    Glöss, Alexia N; Schönbächler, Barbara; Rast, Markus; Deuber, Louis; Yeretzian, Chahan

    2014-01-01

    With the growing demand for high-quality coffee, it is becoming increasingly important to establish quantitative measures of the freshness of coffee, or the loss thereof, over time. Indeed, freshness has become a critical quality criterion in the specialty coffee scene, where the aim is to deliver the most pleasant flavor in the cup, from highest quality beans. A series of intensity ratios of selected volatile organic compounds (VOC) in the headspace of coffee (by gas chromatography-mass spectrometry) were revisited, with the aim to establish robust indicators of freshness of coffee - called freshness indices. Roasted whole beans in four different packaging materials and four commercial capsule systems from the Swiss market were investigated over a period of up to one year of storage time. These measurements revealed three types of insight. First, a clear link between barrier properties of the packaging material and the evolution of selected freshness indices was observed. Packaging materials that contain an aluminum layer offer better protection. Second, processing steps prior to packaging are reflected in the absolute values of freshness indices. Third, differences in the standard deviations of freshness-indices for single serve coffee capsule systems are indicative of differences in the consistency among systems, consistency being an important quality attribute of capsules.

  14. Analysis of experimental measurements of PWR fresh and spent fuel assemblies using Self-Interrogation Neutron Resonance Densitometry

    Energy Technology Data Exchange (ETDEWEB)

    LaFleur, Adrienne M., E-mail: alafleur@lanl.gov; Menlove, Howard O., E-mail: hmenlove@lanl.gov

    2015-05-01

    Self-Interrogation Neutron Resonance Densitometry (SINRD) is a new NDA technique that was developed at Los Alamos National Laboratory (LANL) to improve existing nuclear safeguards measurements for LWR fuel assemblies. The SINRD detector consists of four fission chambers (FCs) wrapped with different absorber filters to isolate different parts of the neutron energy spectrum and one ion chamber (IC) to measure the gross gamma rate. As a result, two different techniques can be utilized using the same SINRD detector unit and hardware. These techniques are the Passive Neutron Multiplication Counter (PNMC) method and the SINRD method. The focus of the work described in this paper is the analysis of experimental measurements of fresh and spent PWR fuel assemblies that were performed at LANL and the Korea Atomic Energy Research Institute (KAERI), respectively, using the SINRD detector. The purpose of these experiments was to assess the following capabilities of the SINRD detector: 1) reproducibility of measurements to quantify systematic errors, 2) sensitivity to water gap between detector and fuel assembly, 3) sensitivity and penetrability to the removal of fuel rods from the assembly, and 4) use of PNMC/SINRD ratios to quantify neutron multiplication and/or fissile content. The results from these simulations and measurements provide valuable experimental data that directly supports safeguards research and development (R&D) efforts on the viability of passive neutron NDA techniques and detector designs for partial defect verification of spent fuel assemblies. - Highlights: • Experimental measurements of PWR fresh and spent FAs were performed with SINRD. • Good agreement of MCNPX and measured results confirmed accuracy of SINRD model. • For fresh fuel, SINRD and PNMC ratios were not sensitive to water gaps of ≤5-mm. • Practical use of SINRD would be in Fork detector to reduce systematic uncertainties.

  15. Characterization of airag collected in Ulaanbaatar, Mongolia with emphasis on isolated lactic acid bacteria

    OpenAIRE

    Suk-Ho Choi

    2016-01-01

    Abstract Background Airag, alcoholic sour-tasting beverage, has been traditionally prepared by Mongolian nomads who naturally ferment fresh mares’ milk. Biochemical and microbiological compositions of airag samples collected in Ulaanbaatar, Mongolia and physiological characteristics of isolated lactic acid bacteria were investigated. Methods Protein composition and biochemical composition were determined using sodium dodecyl sulfate-gel electrophoresis and high performance liquid chromatograp...

  16. Prevention of ligneous conjunctivitis by topical and subconjunctival fresh frozen plasma.

    Science.gov (United States)

    Tabbara, Khalid F

    2004-08-01

    To present a case of ligneous conjunctivitis where the recurrence of membranous conjunctivitis was prevented by subconjunctival and topical instillation of fresh frozen plasma. Interventional case report. A case of ligneous conjunctivitis with multiple recurrences since the age of 3 years developed recurrent membranous conjunctivitis after transconjunctival levator recession. Blood plasminogen activity was determined. The membrane was excised, and the membrane reappeared 4 days later. The patient was treated with excision of the membrane and subconjunctival injection of fresh frozen plasma and topical fresh frozen plasma. Plasminogen activity of the fresh frozen plasma was normal. Plasminogen blood functional activity was 52% (normal is 80%-120%). The patient had complete remission with no recurrences of membranous conjunctivitis after topical and subconjunctival fresh frozen plasma. Prophylactic use of topical and subconjunctival fresh frozen plasma may help in the prevention of membranes in susceptible patients with plasminogen deficiency.

  17. Fresh and Frozen Tissue-Engineered Three-Dimensional Bone–Ligament–Bone Constructs for Sheep Anterior Cruciate Ligament Repair Following a 2-Year Implantation

    Directory of Open Access Journals (Sweden)

    Vasudevan Mahalingam

    2016-10-01

    Full Text Available njuries to the anterior cruciate ligament (ACL often require surgical reconstruction utilizing tendon grafts to restore knee function and stability. Some current graft options for ACL repair are associated with poor long-term outcomes. Our laboratory has fabricated tissue-engineered bone–ligament–bone (BLB constructs that demonstrate native ligament regeneration and advancement toward native ACL mechanical properties in a sheep ACL reconstruction model. Prior work has shown that freezing BLBs as a method of preservation resulted in similar outcomes compared with fresh BLBs after 6-month implantation. The purpose of this study was to evaluate the long-term efficacy of fresh and frozen BLBs. We hypothesized that both fresh and frozen BLBs would show continued regeneration of structural and functional properties toward those of native ACL after a 2-year implantation. Following removal of the native ACL, fresh (n = 2 and frozen (n = 2 BLBs were implanted arthroscopically. After 2 years of recovery, sheep were euthanized and both the experimental and contralateral hindlimbs were removed and radiographs were performed. Explanted knees were initially evaluated for joint laxity and were then further dissected for uniaxial tensile testing of the isolated ACL or BLB. Following mechanical testing, explanted contralateral ACL (C-ACL and BLBs were harvested for histology. Two years post-ACL reconstruction, fresh and frozen BLBs exhibited similar morphological and biomechanical properties as well as more advanced regeneration compared with our 6-month recovery study. These data indicate that an additional 1.5-year regeneration period allows the BLB to continue ligament regeneration in vivo. In addition, freezing the BLBs is a viable option for the preservation of the graft after fabrication.

  18. Diversity of Protease-Producing Bacillus spp. From Fresh Indonesian Tempeh Based on 16S rRNA Gene Sequence

    Directory of Open Access Journals (Sweden)

    Tati Barus

    2017-01-01

    Full Text Available Tempeh is a type of traditional fermented food in Indonesia. The fermentation can be performed by Rhizopus microsporus as a main microorganism. However, Bacillus spp. is found in abundance in tempeh production. Nevertheless, information regarding the diversity of Bacillus spp. in tempeh production has not been reported yet. Therefore, the aim of this investigation was to study the genetic diversity of Bacillus spp. in tempeh production based on the 16S ribosomal RNA sequence. In this study, about 22 of 24 fresh tempeh from Jakarta, Bogor, and Tangerang were used. A total of 52 protease-producing Bacillus spp. isolates were obtained. Based on 16S ribosomal RNA results, all 52 isolates were identified to be similar to B. pumilus, B. subtilis, B. megaterium, B. licheniformis, B. cereus, B. thuringiensis, B. amyloliquefaciens, Brevibacillus brevis, and Bacillus sp. All the identified isolates were divided into two large clusters: 1 a cluster of B. cereus, B. thuringiensis, Bacillus sp., and B. brevis and 2 a cluster of B. pumilus, B. subtilis, B. megaterium, B. licheniformis, and B. amyloliquefaciens. Information about the Bacillus spp. role in determining the quality of tempeh has not been reported and this is a preliminary study of Bacillus spp. from tempeh.

  19. Optimum Shock Isolation

    National Research Council Canada - National Science Library

    Bolotnik, Nikolai

    2001-01-01

    .... Several types of performance criteria for isolation are considered, the most important of which are the peak force transmitted to the body to be isolated and the maximum displacement of the body relative to the base...

  20. Isolated patellofemoral osteoarthritis.

    NARCIS (Netherlands)

    Jonbergen, J.P.W. van; Poolman, R.W.; Kampen, A. van

    2010-01-01

    BACKGROUND AND PURPOSE: The optimal treatment for isolated patellofemoral osteoarthritis is unclear at present. We systematically reviewed the highest level of available evidence on the nonoperative and operative treatment of isolated patellofemoral osteoarthritis to develop an evidenced-based

  1. Comparative study of chemistry compositions and antimicrobial potentials of essential oils and oleoresins from dried and fresh Mentha longifolia L.

    Directory of Open Access Journals (Sweden)

    Sunita Singh

    2015-12-01

    Full Text Available Objective: To investigate the chemical compositions and antimicrobial potentials of the essential oils and oleoresins obtained from fresh and dried Mentha longifolia L. Methods: Gas chromatography and gas chromatography-mass spectrometer techniques were used to determine the profiling of the essential oils and oleoresins. In order to determine the antimicrobial efficacy of the volatile oil and oleoresins, the pathogenic fungus Aspergillus niger (1884, Aspergillus flavus (2479, Fusarium monoliforme (1893, Fusarium graminearum (2088 and Penicillium viridicatum (2007 were undertaken whereas four pathogenic bacteria Bacillus subtilis (1790, Staphylococcus aureus (3103 (Gram-positive, Escherichia coli (1672, Pseudomonas aeruginosa (1942 (Gram-negative were selected for the present study. Food poisoned, inverted Petri plate, agar well diffusion and disk diffusion methods were employed for investigating antimicrobial potentials. Results: Piperitenone oxide, an oxygenated monoterpene, dominated the chemical compositions of essential oils and oleoresins whose compositions varied from 23.5%–87.8%. Both essential oils showed good antifungal activities against Aspergillus and Fusarium species. The antibacterial investigations revealed that Gram-positive bacteria were more sensitive to the essential oils. Conclusions: Drying the fresh herbal materials influences the chemical contents and the biological activities of the essential oils and oleoresins. Such results indicate that essential oils of Mentha longifolia L. can be possible candidates for further investigations to isolate and characterize their active principles as possible new natural preservatives.

  2. Biotransformation of Momordica charantia fresh juice by Lactobacillus plantarum BET003 and its putative anti-diabetic potential

    Directory of Open Access Journals (Sweden)

    Farhaneen Afzal Mazlan

    2015-10-01

    Full Text Available Lactobacillus plantarum BET003 isolated from Momordica charantia fruit was used to ferment its juice. Momordica charantia fresh juice was able to support good growth of the lactic acid bacterium. High growth rate and cell viability were obtained without further nutrient supplementation. In stirred tank reactor batch fermentation, agitation rate showed significant effect on specific growth rate of the bacterium in the fruit juice. After the fermentation, initially abundant momordicoside 23-O-β-Allopyranosyle-cucurbita-5,24-dien-7α,3β,22(R,23(S-tetraol-3-O-β-allopyranoside was transformed into its corresponding aglycone in addition to the emergence of new metabolites. The fermented M. charantia juice consistently reduced glucose production by 27.2%, 14.5%, 17.1% and 19.2% at 15-minute intervals respectively, when compared against the negative control. This putative anti-diabetic activity can be attributed to the increase in availability and concentration of aglycones as well as other phenolic compounds resulting from degradation of glycosidic momordicoside. Biotransformation of M. charantia fruit juice via lactic acid bacterium fermentation reduced its bitterness, reduced its sugar content, produced aglycones and other metabolites as well as improved its inhibition of α-glucosidase activity compared with the fresh, non-fermented juice.

  3. Biotransformation of Momordica charantia fresh juice by Lactobacillus plantarum BET003 and its putative anti-diabetic potential.

    Science.gov (United States)

    Mazlan, Farhaneen Afzal; Annuar, M Suffian M; Sharifuddin, Yusrizam

    2015-01-01

    Lactobacillus plantarum BET003 isolated from Momordica charantia fruit was used to ferment its juice. Momordica charantia fresh juice was able to support good growth of the lactic acid bacterium. High growth rate and cell viability were obtained without further nutrient supplementation. In stirred tank reactor batch fermentation, agitation rate showed significant effect on specific growth rate of the bacterium in the fruit juice. After the fermentation, initially abundant momordicoside 23-O-β-Allopyranosyle-cucurbita-5,24-dien-7α,3β,22(R),23(S)-tetraol-3-O-β-allopyranoside was transformed into its corresponding aglycone in addition to the emergence of new metabolites. The fermented M. charantia juice consistently reduced glucose production by 27.2%, 14.5%, 17.1% and 19.2% at 15-minute intervals respectively, when compared against the negative control. This putative anti-diabetic activity can be attributed to the increase in availability and concentration of aglycones as well as other phenolic compounds resulting from degradation of glycosidic momordicoside. Biotransformation of M. charantia fruit juice via lactic acid bacterium fermentation reduced its bitterness, reduced its sugar content, produced aglycones and other metabolites as well as improved its inhibition of α-glucosidase activity compared with the fresh, non-fermented juice.

  4. Application of Cold Chain Logistics Safety Reliability in Fresh Food Distribution Optimization

    OpenAIRE

    Zou Yifeng; Xie Ruhe

    2013-01-01

    In view of the nature of fresh food’s continuous decrease of safety during distribution process, this study applied safety reliability of food cold chain logistics to establish fresh food distribution routing optimization model with time windows, and solved the model using MAX-MIN Ant System (MMAS) with case analysis. Studies have shown that the mentioned model and algorithm can better solve the problem of fresh food distribution routing optimization with time windows.

  5. Parasitic contamination of fresh vegetables sold at central markets in Khartoum state, Sudan

    OpenAIRE

    Mohamed, Mona Ali; Siddig, Emmanuel Edwar; Elaagip, Arwa Hassan; Edris, Ali Mahmoud Mohammed; Nasr, Awad Ahmed

    2016-01-01

    Background Fresh vegetables are considered as vital nutrients of a healthy diet as they supply the body with essential supplements. The consumption of raw vegetables is the main way for transmission of intestinal parasitic organisms. This study was aimed at detecting the parasitic contamination in fresh vegetables sold in two central open-aired markets in Khartoum state, Sudan. Methods In this prospective cross-sectional study, a total of 260 fresh vegetable samples and 50 water samples used ...

  6. Consumer’s attitude and perception of fresh seafood products: cognitive effects on behaviour buying

    OpenAIRE

    Gouin, Stéphane; Mesnildrey, Lucile; Lesueur, Marie

    2010-01-01

    Since 2004, the French fishery field has had to cope with a levelling off of consumption of fresh seafood products whereas the consumption of processed seafood products is increasing. Nowadays, consumers prefer easier products: fresh deli products, easy to cook or ready to consume. Therefore, fresh seafood products do not seem to fulfil current consumer demand intrinsically (aspect, taste) and extrinsically (cooking methods, use, origin, brand and price). In order to understand the evolution ...

  7. Effect of minimal processing on physiology and quality of fresh-cut potatoes, a review

    OpenAIRE

    Rocculi, Pietro; Romani, Santina; Gomez, Federico; Dalla Rosa, Marco

    2009-01-01

    Fresh-cut fruit and vegetable are minimally processed products that have to maintain their quality (appearance, texture, flavour and nutritive value) similar to those of the fresh product. The fundamental principle underlying the quality of these commodities is that they are metabolic active tissues and, as a consequence, show physiological response to preparation procedures as well as to the environment created in the package in which they are enclosed. Minimal processing for fresh-cut potat...

  8. Potential use of ionizing radiation as quarantine treatment for fresh fruits, vegetables and flowers in Ecuador

    International Nuclear Information System (INIS)

    Munoz, Ricardo

    1990-01-01

    It is presented a brief description of the investigations Technology. Considering the potentiality of the country, to export non traditional and exotic fresh fruits, fresh vegetables, and ornamental fresh cut flowers, studies ares initiated to use ionizing radiation as quarantine treatments. This paper reports the initial data obtained with two species of fruit fly of high incidence in the fruit producer zones of Ecuador, whose stable and permanent microclimates allow to produce a sort of fruits along all year

  9. Effect of substituting fresh-cut perennial ryegrass with fresh-cut white clover on bovine milk fatty acid profile.

    Science.gov (United States)

    Stergiadis, Sokratis; Hynes, Deborah N; Thomson, Anna L; Kliem, Kirsty E; Berlitz, Carolina Gb; Günal, Mevlüt; Yan, Tianhai

    2018-03-06

    Including forage legumes in dairy systems can help address increasing environmental/economic concerns about perennial ryegrass monoculture pastures. This work investigated the effect of substituting fresh-cut grass with increasing quantities of fresh-cut white clover (WC) on milk fatty acid (FA) profile and transfer efficiency of dietary linoleic (LA) and α-linolenic (ALNA) acids to milk fat. Three groups of three crossbred dairy cows were used in a 3 × 3 crossover design. Dietary treatments were 0 g kg -1 WC + 600 g kg -1 grass, 200 g kg -1 WC + 400 g kg -1 grass, and 400 g kg -1 WC + 200 g kg -1 grass. All treatments were supplemented with 400 g kg -1 concentrates on a dry matter basis. Cows had a 19-day adaptation period to the experimental diet before a 6-day measurement period in individual tie stalls. Increasing dietary WC did not affect dry matter intake, milk yield or milk concentrations of fat, protein or lactose. Milk polyunsaturated FA concentrations (total n-3, total n-6, LA and ALNA) and transfer efficiency of LA and ALNA were increased with increasing dietary WC supply. Inclusion of WC in pastures may increase concentrations of nutritionally beneficial FA, without influencing milk yield and basic composition, but any implications on human health cannot be drawn. © 2018 The Authors. Journal of the Science of Food and Agriculture published by JohnWiley & Sons Ltd on behalf of Society of Chemical Industry. © 2018 The Authors. Journal of the Science of Food and Agriculture published by JohnWiley & Sons Ltd on behalf of Society of Chemical Industry.

  10. Comparison of the nutrient content of fresh fruit juices vs commercial fruit juices.

    Science.gov (United States)

    Densupsoontorn, Narumon; Jirapinyo, Pipop; Thamonsiri, Nuchnoi; Wongarn, Renu; Phosuya, Panarat; Tritiprat, Amornrat; Patraarat, Siriphan; Pidatcha, Pannee; Suwannthol, Lerson

    2002-08-01

    To compare the types and quantities of carbohydrate, electrolytes, pH and osmolarity of fresh fruit juices and commercial fruit juices. Forty kinds of fresh fruits available in Thai markets were analyzed for types and quantities of carbohydrate, electrolyte, pH and osmolarity and compared with previously obtained data for commercial fruit juices. Most fresh fruit juices did not contain sucrose, whereas, commercial fruit juices mostly have sucrose in the range of 3-112 g/L. Although both fruit juices were acidic (pH varied from 3.6-6.7 and 3.2-5.8 of fresh juice and commercial juice), fresh fruit juices had a more neutral pH than commercial fruit juices. Apple, guava, orange, pear, and pineapple juices from commercial fruit juices had a high osmolarity compared with fresh fruit juices. All types of fresh fruit juices contained less sodium than commercial ones, whereas, most fresh fruit juices contained more potassium, phosphorus, and magnesium than commercial fluids. The nutrient content of fresh fruit juices and commercial fruit juices from the same kinds of fruits are not the same, possibly due to the manufacturing process. Therefore, physicians should know the composition of fruit juices in order to advise patients properly.

  11. The impact of hydration changes in fresh bio-tissue on THz spectroscopic measurements

    International Nuclear Information System (INIS)

    Png, G M; Ng, B W-H; Mickan, S P; Abbott, D; Choi, J W; Zhang, X-C

    2008-01-01

    We present a study of how residual hydration in fresh rat tissue samples can vastly alter their extracted terahertz (THz) optical properties and influence their health assessment. Fresh (as opposed to preserved) tissue most closely mimics in vivo conditions, but high water content creates many challenges for tissue handling and THz measurement. Our THz measurements of fresh tissue over time highlight the effect of tissue hydration on tissue texture and dimension, the latter directly influencing the accuracy of calculated optical properties. We then introduce lyophilization (freeze drying) as a viable solution for overcoming hydration and freshness problems. Lyophilization removes large amounts of water while retaining sample freshness. In addition, lyophilized tissue samples are easy to handle and their textures and dimensions do not vary over time, allowing for consistent and stable THz measurements. A comparison of lyophilized and fresh tissue shows for the first time that freeze drying may be one way of overcoming tissue hydration issues while preserving tissue cellular structure. Finally, we compare THz measurements from fresh tissue against necrotic tissue to verify freshness over time. Indeed, THz measurements from fresh and necrotic tissues show marked differences

  12. Recent Developments in Film and Gas Research in Modified Atmosphere Packaging of Fresh Foods.

    Science.gov (United States)

    Zhang, Min; Meng, Xiangyong; Bhandari, Bhesh; Fang, Zhongxiang

    2016-10-02

    Due to the rise of consumer's awareness of fresh foods to health, in the past few years, the consumption of fresh and fresh-cut produces has increased sturdily. Modified atmosphere packaging (MAP) possesses a potential to become one of the most appropriate technologies for packaging fresh and fresh-cut produces. The MAP has advantages of extending the shelf-life, preserving or stabilizing the desired properties of fresh produces, and convenience in handing and distribution. The success of MAP-fresh foods depends on many factors including types of fresh foods, storage temperature and humidity, gas composition, and the characteristics of package materials. This paper reviews the recent developments highlighting the most critical factors of film and gas on the quality of MAP fresh foods. Although the innovations and development of food packaging technology will continue to promote the development of novel MAP, concentrated research and endeavors from scientists and engineers are still important to the development of MAP that focuses on consumers' requirements, enhancing product quality, environmental friendly design, and cost-effective application.

  13. Inactivation of Escherichia coli inoculated onto fresh-cut chopped cabbage using electron-beam processing.

    Science.gov (United States)

    Grasso, Elizabeth M; Uribe-Rendon, Roberto M; Lee, Ken

    2011-01-01

    During the past decade there were more than 50 reported outbreaks involving leafy green vegetables contaminated with foodborne pathogens. Leafy greens, including cabbage, are fresh foods rarely heated before consumption, which enables foodborne illness. The need for improved safety of fresh food drives the demand for nonthermal food processes to decrease the risk of pathogens while maintaining fresh quality. This study examines the efficacy of electron-beam (e-beam) irradiation in decreasing indigenous microflora on fresh-cut cabbage and determines the optimal dosage to pasteurize fresh-cut cabbage inoculated with Escherichia coli K-12. Fresh-cut cabbage (100 g) was inoculated with ∼8 log E. coli K-12 and e-beam irradiated at doses of 0, 1.0, 2.3, or 4.0 kGy. At 2.3 kGy there was 7-log reduction of E. coli K-12 in the fresh-cut cabbage. The D(10)-value for E. coli K-12 in fresh-cut cabbage was 0.564 kGy. E-beam irradiation is thus a viable nonthermal treatment that extends the shelf life and increases the safety of fresh cabbage by reducing or eliminating indigenous microflora and unwanted pathogens.

  14. Chemical and Quantitive Study of Hepatotoxins from Fresh Water ...

    African Journals Online (AJOL)

    Toxicity of the freeze-dried micro-aquatic planktonic cyanobacterium Oscillatoria agardhii, dominating and isolated from Makkah –KSA was studied. Microcystins were detected from freeze-dried cells using high pressure liquid chromatography HPLC. The histopathological examination of mice liver injected on week basis ...

  15. Two novel prenylated kaempferol derivatives from fresh bud's fur of Platanus acerifolia and their anti-proliferative activities.

    Science.gov (United States)

    Zuo, Bo; Liao, Zhi-Xin; Xu, Chen; Liu, Chao

    2016-01-06

    Two novel prenylated kaempferol derivatives (1, 2), together with seven known metabolites were isolated from ethanol extract of fresh Platanus acerifolia bud's fur by multistep chromatographic processing. Structure of compounds 1 and 2 was confirmed by 1D, 2D NMR spectra and HR-ESI-MS. In addition, compound 1 was further analysed by X-ray crystallography. Anti-proliferative activities in vitro against human breast carcinoma (MCF-7) and human hepatocellular carcinoma (Hep-G2) cell lines for compound 1, 2 and 8 were evaluated. Compound 1 exhibited cytotoxic activity towards MCF-7 and Hep-G2 cell lines with the IC 50 values 38.2 and 39.5 μM, respectively. Moreover, compound 2 showed weak cytotoxic activities against the two cell lines.

  16. Structure Elucidation of A New Coumarin Type Compound, Alternamin, Isolated from the Plant (Murraya Alternans (Kurz) Swingle) Having The Antidote Activity on Snake Venoms

    International Nuclear Information System (INIS)

    Hla Myo Min; Mya Aye

    2004-06-01

    The structure of a new coumarin type compound isolated from the entitled species was elucidated by the full spectral analysis consisting of FTIR, 1H NMR, DQF COSY, 13C NMR, DEPT, EIMS (HR-EIMS), HMQC and HMBC. The antidote activities of the fresh juice and the ethanolic extract of the plant, and the isolated compound alternamin were also determined

  17. A comparison for acid production, proteolysis, autolysis and inhibitory properties of lactic acid bacteria from fresh and mature Feta PDO Greek cheese, made at three different mountainous areas.

    Science.gov (United States)

    Bozoudi, Despina; Kotzamanidis, Charalambos; Hatzikamari, Magdalini; Tzanetakis, Nikolaos; Menexes, George; Litopoulou-Tzanetaki, Evanthia

    2015-05-04

    Isolates of NSLAB were obtained from fresh (58 isolates) and mature (38) Feta cheese made at household level in three different mountainous areas, in order to study the effect of the area of production on NSLAB composition and their technological characteristics. Results obtained by SDS-PAGE of whole-cell proteins indicated that the microflora of the fresh cheese was composed of either lactococci (areas 1, 2), or lactococci and enterococci (area 3). The NSLAB microflora of mature cheese was composed almost entirely of lactobacilli species, differing according to the area of production. Species allocation by the SDS-PAGE method was confirmed by sequencing representative strains. Lactococci of cheese made in area 1 exhibited a narrow spectrum of antibacterial activity compared to isolates from areas 2 and 3, while for lactobacilli from all three areas a similar spectrum was noticed. Lactococci from area 2 exhibited higher (Pautolysis at pH5.1. Mean acidifying activity of lactobacilli after 6h was for strains of area 3>2=1. The strains from areas 1 and 3 degraded preferentially αs-CN, while a clear preference towards β-CN was noticed for strains of area 2; their mean proteolytic activity was for strains of area 1 higher (Pautolysis allowed the distinction of lactococci according to their derivation area enabling the selection of appropriate strains as starters for cheese production in each area. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Efficacy of various protein-based coating on enhancing the shelf life of fresh eggs during storage.

    Science.gov (United States)

    Caner, Cengiz; Yüceer, Muhammed

    2015-07-01

    The effectiveness of various coatings (whey protein isolate [WPI], whey protein concentrate [WPC], zein, and shellac) on functional properties, interior quality, and eggshell breaking strength of fresh eggs were evaluated during storage at 24 °: C for 6 weeks. Coatings and storage time had significant effects on Haugh unit, yolk index, albumen pH, dry matter (DMA), relative whipping capacity (RWC), and albumen viscosity. Uncoated eggs had higher albumen pH (9.56) and weight loss, and lower albumen viscosity (5.73), Haugh unit (HU), and yolk index (YI) during storage. Among the coated eggs, the shellac and zein coated eggs had the highest value of albumen viscosity (27.26 to 26.90), HU (74.10 to 73.61), and YI (44.84 to 44.63) after storage. Shellac (1.44%) was more effective in preventing weight loss than WPC (4.59%), WPI (4.60%), and zein (2.13%) coatings. Uncoated eggs had the higest value (6.71%) of weight lost. All coatings increased shell strength (5.18 to 5.73 for top and 3.58 to 4.71 for bottom) significantly (P eggs (4.70 for top and 3.15 for bottom). The functional properties such as albumen DMA (14.50 to 16.66 and 18.97 for uncoated) and albumen RWC (841 to 891 and 475 for uncoated) of fresh eggs can be preserved during storage when they are coated. The shellac and zein coatings were more effective for maintaining the internal quality of fresh eggs during storage. Fourier transform near infrared (FT-NIR) in the 800 to 2500 nm reflection spectra were used to quantify the contents of the fresh eggs at the end of storage. Eggs coated with shellac or zein displayed a higher absorbance at 970 and 1,197 nm respectively (OH vibration of water) compared with those coated with WPI or WPC and the uncoated group at the end of storage. The coatings improved functional properties and also shell strength and could be a viable alternative technology for maintaining the internal quality of eggs during long-term storage. This study highlights the promising use of

  19. Understanding Freshness Perception from the Cognitive Mechanisms of Flavor: The Case of Beverages

    Science.gov (United States)

    Roque, Jérémy; Auvray, Malika; Lafraire, Jérémie

    2018-01-01

    Freshness perception has received recent consideration in the field of consumer science mainly because of its hedonic dimension, which is assumed to influence consumers’ preference and behavior. However, most studies have considered freshness as a multisensory attribute of food and beverage products without investigating the cognitive mechanisms at hand. In the present review, we endorse a slightly different perspective on freshness. We focus on (i) the multisensory integration processes that underpin freshness perception, and (ii) the top–down factors that influence the explicit attribution of freshness to a product by consumers. To do so, we exploit the recent literature on the cognitive underpinnings of flavor perception as a heuristic to better characterize the mechanisms of freshness perception in the particular case of beverages. We argue that the lack of consideration of particular instances of flavor, such as freshness, has resulted in a lack of consensus about the content and structure of different types of flavor representations. We then enrich these theoretical analyses, with a review of the cognitive mechanisms of flavor perception: from multisensory integration processes to the influence of top–down factors (e.g., attentional and semantic). We conclude that similarly to flavor, freshness perception is characterized by hybrid content, both perceptual and semantic, but that freshness has a higher-degree of specificity than flavor. In particular, contrary to flavor, freshness is characterized by specific functions (e.g., alleviation of oropharyngeal symptoms) and likely differs from flavor with respect to the weighting of each sensory contributor, as well as to its subjective location. Finally, we provide a comprehensive model of the cognitive mechanisms that underlie freshness perception. This model paves the way for further empirical research on particular instances of flavor, and will enable advances in the field of food and beverage cognition

  20. Understanding Freshness Perception from the Cognitive Mechanisms of Flavor: The Case of Beverages

    Directory of Open Access Journals (Sweden)

    Jérémy Roque

    2018-01-01

    Full Text Available Freshness perception has received recent consideration in the field of consumer science mainly because of its hedonic dimension, which is assumed to influence consumers’ preference and behavior. However, most studies have considered freshness as a multisensory attribute of food and beverage products without investigating the cognitive mechanisms at hand. In the present review, we endorse a slightly different perspective on freshness. We focus on (i the multisensory integration processes that underpin freshness perception, and (ii the top–down factors that influence the explicit attribution of freshness to a product by consumers. To do so, we exploit the recent literature on the cognitive underpinnings of flavor perception as a heuristic to better characterize the mechanisms of freshness perception in the particular case of beverages. We argue that the lack of consideration of particular instances of flavor, such as freshness, has resulted in a lack of consensus about the content and structure of different types of flavor representations. We then enrich these theoretical analyses, with a review of the cognitive mechanisms of flavor perception: from multisensory integration processes to the influence of top–down factors (e.g., attentional and semantic. We conclude that similarly to flavor, freshness perception is characterized by hybrid content, both perceptual and semantic, but that freshness has a higher-degree of specificity than flavor. In particular, contrary to flavor, freshness is characterized by specific functions (e.g., alleviation of oropharyngeal symptoms and likely differs from flavor with respect to the weighting of each sensory contributor, as well as to its subjective location. Finally, we provide a comprehensive model of the cognitive mechanisms that underlie freshness perception. This model paves the way for further empirical research on particular instances of flavor, and will enable advances in the field of food and

  1. Prompt burst energetics experiments: fresh oxide/sodium series

    International Nuclear Information System (INIS)

    Reil, K.O.; Young, M.F.

    1978-08-01

    A series of in-pile experiments has been performed to provide information on thermal energy to work conversion under prompt burst excursion (PBE) conditions. These consisted of single pin tests using fresh uranium oxide or uranium carbide fuel in a capsule geometry, with either stagnant sodium or helium in the coolant channel. The experiments were irradiated with single or double pulses in the Annular Core Pulse Reactor (ACPR) to provide energy depositions up to 2900 J/g. This report covers the seven single and five double pulse UO 2 sodium-in tests. Experimental data includes pressure and linear motion transducer histories, measured work-energy conversion efficiencies, and post-irradiation examination. Analysis includes derived work-energy conversion efficiencies (up to 0.54%), pin failure modeling, hydrodynamic analysis of pressure pulse propagation in the channel, and piston stopping effects. Initial pressure events in the single pulse experiments appear to be dominated by fuel vapor pressure. Definite fuel-coolant interactions were observed in several experiments, including some that were coincident with stopping of the linear motion transducer piston, suggesting a possible triggering effect by the deceleration pressure

  2. From fresh heterologous oocyte donation to autologous oocyte banking.

    Science.gov (United States)

    Stoop, D

    2012-01-01

    Today, oocyte donation has become well established, giving rise to thousands of children born worldwide annually. The introduction of oocyte cryopreservation through vitrification allows the introduction of egg banking, improving the efficiency and comfort of oocyte donation. Moreover, the vitrification technique can now enable autologous donation of oocytes to prevent future infertility. We evaluated fresh heterologous oocyte donation in terms of obstetrical and perinatal outcome as well as of the reproductive outcome of past donors. We then evaluated the efficiency of a closed vitrification device and its clinical applications within ART. Thirdly, we evaluated the opinion of women with regard to preventive egg freezing and the efficiency of a human oocyte in relation to age. Oocyte donation is associated with an increased risk of first trimester bleeding and pregnancy induced hypertension. Donating oocytes does not seem to increase the likelihood for a later need of fertility treatment. The chance of an oocyte to result in live birth (utilization rate) in women women would consider safeguarding their reproductive potential through egg freezing or are at least open to the idea. The introduction of efficient oocyte cryopreservation has revolutionized oocyte donation through the establishment of eggbank donation. The technique also enables women to perform autologous donation after preventive oocyte storage in order to circumvent their biological clock.

  3. Waste tyre rubberized concrete: properties at fresh and hardened state.

    Science.gov (United States)

    Aiello, M A; Leuzzi, F

    2010-01-01

    The main objective of this paper is to investigate the properties of various concrete mixtures at fresh and hardened state, obtained by a partial substitution of coarse and fine aggregate with different volume percentages of waste tyres rubber particles, having the same dimensions of the replaced aggregate. Workability, unit weight, compressive and flexural strength and post-cracking behaviour were evaluated and a comparison of the results for the different rubcrete mixtures were proposed in order to define the better mix proportions in terms of mechanical properties of the rubberized concrete. Results showed in this paper were also compared to data reported in literature. Moreover, a preliminary geometrical, physical and mechanical characterization on scrap tyre rubber shreds was made. The rubberized concrete mixtures showed lower unit weight compared to plain concrete and good workability. The results of compressive and flexural tests indicated a larger reduction of mechanical properties of rubcrete when replacing coarse aggregate rather than fine aggregate. On the other hand, the post-cracking behaviour of rubberized concrete was positively affected by the substitution of coarse aggregate with rubber shreds, showing a good energy absorption and ductility indexes in the range observed for fibrous concrete, as suggested by standard (ASTM C1018-97, 1997). 2010 Elsevier Ltd. All rights reserved.

  4. Application of tidal energy for purification in fresh water lake

    Directory of Open Access Journals (Sweden)

    Rho-Taek Jung

    2015-01-01

    Full Text Available In order to preserve the quality of fresh water in the artificial lake after the reclamation of an intertidal flat at the mouth of a river, we suggest two novel methods of water purification by using tidal potential energy and an enclosed permeable embankment called an utsuro (Akai et al., 1990 in the reclaimed region. One method uses an inflatable bag on the seabed within an utsuro, while the other uses a moored floating barge out of a dyke. Each case employs a subsea pipe to allow flow between the inside and outside of the utsuro. The change in water level in the utsuro, which is pushed through the pipe by the potential energy outside, caused circulation in the artificial lake. In this paper, we analyzed the inflatable bag and floating barge motion as well as the pipe flow characteristics and drafts as given by a harmonic sea level, and compared the theoretical value with an experimental value with a simple small model basin. The numerical calculation based on theory showed good agreement with experimental values.

  5. Improving Scotland's health: time for a fresh approach?

    Science.gov (United States)

    Stone, D H

    2012-05-01

    Scotland's health remains the worst in the UK. There are several probable reasons for this. Of those that are amenable to change, health improvement policy has been excessively preoccupied with targeting individuals perceived to be 'at risk' rather than adopting a whole population perspective. Environmental as opposed to behavioural approaches to health improvement have been relatively neglected. To meet the challenge of Scotland's poor health more effectively in the future, new strategic thinking is necessary. Three initial steps are required: recognize that current approaches are inadequate and that fresh ideas are needed; identify the principles that should underlie future strategy development; translate these principles into achievable operational objectives. Five principles of a revitalized strategy to improve the health of Scotland in the future are proposed. These are start early and sustain effort; create a healthy and safe environment; reduce geographical as well as social inequalities in health; adopt an evidence-based approach to public health interventions; use epidemiology to assess need, plan interventions and monitor progress. These principles may then be translated into achievable operational policy and practice objectives.

  6. Impact of ionisation on the conservation of fresh stuffed pasta

    International Nuclear Information System (INIS)

    Ben Dhaou, Thouraya

    2012-01-01

    Fresh stuffed pasta are highly appreciated by consumers due to their specific taste and flavor. However, they are perishable foodstuffs having a short shelf life. The aim of this work was to extend their shelf life using three doses of ionizing gamma 1, 3 and 5 kGy. Studing the impact of this treatment on the microbiological (FAMT, E-coli, Staphylococcus coagulase, Sallmonelle, yeasts and molds), physicochemical (pH, water content and water activity), sensory (color) and cooking qualities (swelling, cooking loss and spalling) of the product showed the effectiveness of its doses toward the spoilage and pathogenic flora. However, organoleptic and cooking qualities were significantly affected by the doses 3 and 5 kGy. Only the dose 1 kGy could improve the microbiological quality without influencing the organoleptic and culinary qualities of the product. The shelf life prediction according to ASLT (Accelerated Shelf Life Testing) method of different samples confirmed the effectiveness of the dose 1 kGy which gave the highest shelf life value (62 days). As the shelf life of un-ionized product is 45 days, the 1 kGy dose has allowed extending the shelf life of 17 days.

  7. Antioxidant properties of natural substances in irradiated fresh poultry

    International Nuclear Information System (INIS)

    Mahrour, A.; Lacroix, M.; Nketsa-Tabiri, J.; Calderon, N.; Gagnon, M.

    1998-01-01

    This study was undertaken to determine if a combined treatment (marinating in natural plant extracts or vacuum) with irradiation could have a synergistic effect, in order to prevent the lipid oxidation resulting in the development of undesirable flavours. The fresh chicken legs were irradiated at 0,3 and 5 kGy. The fatty acids composition of lipids was identified using gas liquid chromatography. The effect of irradiation treatment combined with a pre-treatment on the fatty acids composition was followed. The day after irradiation, ten panallists were asked to evaluate, using the instruction scaling, the overall appearance, the odor, the flavor and the overall acceptability of the samples. The major fatty acids identified in lipids were oleic acid, palmitic acid, palmitoleic acid and stearic acid. Pre-treatments have a significant effect on linoleic acid (C18:2) and higher fatty acids. The unsaturated fatty acids derived from phospholipids appeared to be more affected by the irradiation dose: however, marinating have better protection on C18:2 derived from phospholipids. The results of sensory evaluation have shown a significant better odor and flavor for the irradiated chicken at 5 kGy than the control. No significant difference have been found between the marinated chicken, the chicken irradiated under vacuum and the control

  8. Acceptance and storage of fresh cheese made with essential oils

    Directory of Open Access Journals (Sweden)

    Joelmir Grassi Presente

    2017-08-01

    Full Text Available The aim of this work was to evaluate the acceptance and conservation of Minas fresh cheese with essential oils added of oregano and ginger in its formulation. The quality of the milk used as raw material was evaluated for pH, acidity, alizarol, total solids, density, and total microbial load. The cheeses produced were characterized as pH, acidity, moisture, lipids, proteins and ashes. The cheeses were also evaluated by sensorial affective tests using hedonic and attitude scales, in order to determine the acceptance and purchase intention by judges. The count of total aerobic mesophilic microorganisms was used to estimate the shelf-life of cheeses. The milk used as raw material is presented within the quality standards required by legislation. The cheeses made with essential oils showed pH and acidity around 6.9 and 0.87%, respectively, 57.6% moisture, 31.3% lipids, 11.4% protein and 0.9% ash. The cheese added essential oil of oregano and the control cheese were those given by the judges the best values for acceptance (7.5 and 7.6, respectively and purchase intention (4.2 and 4.4 respectively. Regarding the estimated shelf-life, the cheeses added essential oil of oregano and ginger had lower overall microbial load values compared to the control (no oil and mixed (two oils addition, presented counts values with up 106 UFC/g only from the 28th day of storage.

  9. Practical aspects of sea and fresh-water algae utilization

    International Nuclear Information System (INIS)

    Nyanishkene, V.B.; Zlobin, V.S.; Zheleznyakova, O.V.

    1988-01-01

    Regularities of sea and fresh-water microalgae cultivation, revealed during the operation of industrial plants, are presented in detail. The study of the Platymonas viridis cultivation mechanisms has shown the possibility of active intervention into the cycle of intense reproduction of the biomass. Among physical factors of the medium, which influence the reproduction of the industrial strain of Platymonas viridis, the effect of the red light, ultraviolet radiation, ultrahigh frequency fields and gamma radiation upon the cell division of these microalgae has been investigated. It has been shown that the effect of gamma radiation on the cell division of Platymonas viridis, other bacteria and protozoans is inhibiting. In the experiments using the radionuclides strontium-90 and cerium-144 as indicators of metabolism the dependence of the radionuclide accumulation factor on the quantity of stable lead as well as the effect of the temperature on the accumulation process have been studied. The coefficients of 90 Sr and 144 Ce accumulation by Nitellopsis obtusa cell compartments depending on stable lead and temperature are presented. 200 refs.; 21 figs.; 54 tabs

  10. Antifungal Edible Coatings for Fresh Citrus Fruit: A Review

    Directory of Open Access Journals (Sweden)

    Lluís Palou

    2015-12-01

    Full Text Available According to their origin, major postharvest losses of citrus fruit are caused by weight loss, fungal diseases, physiological disorders, and quarantine pests. Cold storage and postharvest treatments with conventional chemical fungicides, synthetic waxes, or combinations of them are commonly used to minimize postharvest losses. However, the repeated application of these treatments has led to important problems such as health and environmental issues associated with fungicide residues or waxes containing ammoniacal compounds, or the proliferation of resistant pathogenic fungal strains. There is, therefore, an increasing need to find non-polluting alternatives to be used as part of integrated disease management (IDM programs for preservation of fresh citrus fruit. Among them, the development of novel natural edible films and coatings with antimicrobial properties is a technological challenge for the industry and a very active research field worldwide. Chitosan and other edible coatings formulated by adding antifungal agents to composite emulsions based on polysaccharides or proteins and lipids are reviewed in this article. The most important antifungal ingredients are selected for their ability to control major citrus postharvest diseases like green and blue molds, caused by Penicillium digitatum and Penicillium italicum, respectively, and include low-toxicity or natural chemicals such as food additives, generally recognized as safe (GRAS compounds, plant extracts, or essential oils, and biological control agents such as some antagonistic strains of yeasts or bacteria.

  11. Tensile and compressive properties of fresh human carotid atherosclerotic plaques.

    LENUS (Irish Health Repository)

    Maher, Eoghan

    2009-12-11

    Accurate characterisation of the mechanical properties of human atherosclerotic plaque is important for our understanding of the role of vascular mechanics in the development and treatment of atherosclerosis. The majority of previous studies investigating the mechanical properties of human plaque are based on tests of plaque tissue removed following autopsy. This study aims to characterise the mechanical behaviour of fresh human carotid plaques removed during endarterectomy and tested within 2h. A total of 50 radial compressive and 17 circumferential tensile uniaxial tests were performed on samples taken from 14 carotid plaques. The clinical classification of each plaque, as determined by duplex ultrasound is also reported. Plaques were classified as calcified, mixed or echolucent. Experimental data indicated that plaques were highly inhomogeneous; with variations seen in the mechanical properties of plaque obtained from individual donors and between donors. The mean behaviour of samples for each classification indicated that calcified plaques had the stiffest response, while echolucent plaques were the least stiff. Results also indicated that there may be a difference in behaviour of samples taken from different anatomical locations (common, internal and external carotid), however the large variability indicates that more testing is needed to reach significant conclusions. This work represents a step towards a better understanding of the in vivo mechanical behaviour of human atherosclerotic plaque.

  12. Profile and effects of consumer involvement in fresh meat.

    Science.gov (United States)

    Verbeke, Wim; Vackier, Isabelle

    2004-05-01

    This study investigates the profile and effects of consumer involvement in fresh meat as a product category based on cross-sectional data collected in Belgium. Analyses confirm that involvement in meat is a multidimensional construct including four facets: pleasure value, symbolic value, risk importance and risk probability. Four involvement-based meat consumer segments are identified: straightforward, cautious, indifferent, and concerned. Socio-demographic differences between the segments relate to gender, age and presence of children. The segments differ in terms of extensiveness of the decision-making process, impact and trust in information sources, levels of concern, price consciousness, claimed meat consumption, consumption intention, and preferred place of purchase. The two segments with a strong perception of meat risks constitute two-thirds of the market. They can be typified as cautious meat lovers versus concerned meat consumers. Efforts aiming at consumer reassurance through quality improvement, traceability, labelling or communication may gain effectiveness when targeted specifically to these two segments. Whereas straightforward meat lovers focus mainly on taste as the decisive criterion, indifferent consumers are strongly price oriented.

  13. Birefringence in heat-mechanical modified freshly moulded polyester fibers

    Energy Technology Data Exchange (ETDEWEB)

    Velev, V; Dimov, T; Popov, A; Denev, Y; Hristov, H; Angelov, T; Markova, K; Zagortcheva, M; Arhangelova, N; Uzunov, N, E-mail: v.velev@shu-bg.ne

    2010-11-01

    The article submits new experimental data concerning to the role of combined thermo-mechanical treatments on the structural development of freshly moulded uncrystallized but crystallizable poly (ethylene terephthalate) (PET) fibers. The object of the present work is PET as a thermoplastic polymer with a large practical application. The report is devoted to the influence of the heat-mechanical modification temperature on the structure rearrangement in uniaxially orientated amorphous PET. The heat-mechanical modification of the investigated yarns and the optical measurements were realized by specialized gears constructed and built in the author's laboratories. The fibers heat-mechanical modification includes samples annealing at constant temperature above their glass transition temperature (T{sub g}) without strain stress. The yarn annealing has been followed from well defined uniaxially strain-loading with values from 0 MPa up to 30 MPa during two minutes. The optical measurements were carried out by an optical system using a polarization microscope and a CCD camera. The obtained experimental data has been analyzed by Mocha-1.2 (Jandel Scientific) software. There are established dependences between the heat-mechanical modification mode and the structural rearrangements running in the studied PET samples.

  14. Study of dielectric properties of adulterated milk concentration and freshness

    Science.gov (United States)

    Jitendra Murthy, V.; Sai Kiranmai, N.; Kumar, Sanjeev

    2017-08-01

    The knowledge of dielectric properties may hold a potential to develop a new technique for quality evaluation of milk. The dielectric properties of water diluted cow’s milk with milk concentration from 70 percent to 100 percent stored during 36hour storage at 22°C and 144 hour at 5°C were measured at room temperature for frequencies ranging from 10 to 4500 MHz and at low, high & at microwave frequencies using X band bench and open-ended coaxial-line probe technology, along with electrical conductivity. The raw milk had the lowest dielectric constant (ɛ‧) when the frequency was higher than about 20M.Hz, and had the highest loss (ɛ″) or decepation factor tan (δ) at each frequency. The penetration depth (dp) increased with decreasing frequency, water content and storage time, which was large enough to detect dielectric properties changes in milk samples and provide large scale RF pasteurization processes. The loss factor can be an indicator in predicting milk concentration and freshness.

  15. Transfer factor for 137Cs in fresh water aquatic environment

    International Nuclear Information System (INIS)

    Varughese, K.G.; Ramkumar, S.; John, Jaison T.; Rajan, M.P.; Gurg, R.P.

    2002-01-01

    137 Cs is one of the most abundant radionuclides produced in nuclear fission and due to its long radiological half-life and chemical similarity to potassium it has greater biological significance. Radioactive waste materials generated at nuclear facilities are generally disposed within the plant premises under its administrative control for effective radiation protection practices. However trace quantities of radionuclides are released into the environment through liquid and gaseous releases under the guidelines of regulatory agencies. The concentration of these radioactive elements in the environment is not detectable under normal circumstances due to the large dispersion and dilutions available in the environment. But these radionuclides can get accumulated in environmental matrices like silt, weed etc. and indicate the presence of radioactivity in the environment. This paper presents the results of a face-controlled studies conducted at Environmental Survey Laboratories at the Rajasthan Atomic Power Station (RAPS) and Kakrapar Atomic Power Station (KAPS) to estimate distribution of low-level radioactivity in the fresh water system. An attempt has been made to derive the Transfer Factor for 137 Cs in fish, weed, and silt and to evaluate the concentration of 137 Cs in water samples, which is otherwise not detectable under normal procedure of measurement. (author)

  16. Flow conditions of fresh mortar and concrete in different pipes

    International Nuclear Information System (INIS)

    Jacobsen, Stefan; Haugan, Lars; Hammer, Tor Arne; Kalogiannidis, Evangelos

    2009-01-01

    The variation in fresh concrete flow rate over the pipe cross section was investigated on differently coloured and highly flowable concrete mixes flowing through pipes of different materials (rubber, steel, acryl). First, uncoloured (gray) concrete was poured through the pipe and the pipe blocked. Similar but coloured (black) concrete was then poured into the pipe filled with gray concrete, flowing after the gray concrete for a while before being blocked and hardened. The advance of the colouring along the pipe wall (showing boundary flow rate) was observed on the moulded concrete surface appearing after removing the pipe from the hardened concrete. The shapes of the interfaces between uncoloured and coloured concrete (showing variation of flow rate over the pipe cross section) were observed on sawn surfaces of concrete half cylinders cut along the length axes of the concrete-filled pipe. Flow profiles over the pipe cross section were clearly seen with maximum flow rates near the centre of the pipe and low flow rate at the pipe wall (typically rubber pipe with reference concrete without silica fume and/or stabilizers). More plug-shaped profiles, with long slip layers and less variation of flow rate over the cross section, were also seen (typically in smooth acrylic pipes). Flow rate, amount of concrete sticking to the wall after flow and SEM-images of pipe surface roughness were observed, illustrating the problem of testing full scale pumping.

  17. Fresh frozen bone in oral and maxillofacial surgery

    Directory of Open Access Journals (Sweden)

    Luigi Fabrizio Rodella

    2015-06-01

    Full Text Available The aim of the current study was to review the use of fresh frozen bone (FFB in oral and maxillofacial surgery. We performed a review of the articles published in the literature between 1976 and May 2014 analyzing three medical databases (PubMed, Cochrane Library, and Embase and using specific search terms. Literature analysis on FFB applications in oral and maxillofacial surgery revealed 47 articles between 1976 and May 2014. There are 46 clinical articles and one review. Clinical articles are represented by 22 case reports and case series and 24 retrospective studies. Classifying the scientific production by year of publication, it is evident that especially during the last 6 years there was an increase of FFB graft use in oral and maxillofacial approaches. The literature analysis on FFB's use shows that its application in oral and maxillofacial surgery began slowly in 1992 with Perrott and since 2006 it had a real development. The recent significant increase emphasizes the importance of FFB for bone regeneration in oral and maxillofacial surgery. This review found consistent evidence of FFB's use increase in oral and maxillofacial surgery suggesting a valid instrument for bone regeneration. To date, risks connected to the infections' transmission and to the immunogenic potential are extremely low and could be considered practically absent. So, this is an important alternative in the preimplant reconstructive surgery.

  18. Antibiotic resistance of Verotoxigenic Escherichia coli isolated from vegetables

    Directory of Open Access Journals (Sweden)

    mojtaba boniadian

    2017-01-01

    Full Text Available Introduction: Human gastrointestinal disease caused by verotoxigenic Escherichia coli has been diagnosed for recent decades. Escherichia coli O157:H7 is the most important serotype of verotoxigenic Escherichia coli that cause hemolytic uremic syndrome and hemorrhagic colitis in humans. This study was conducted to determine the occurrence of verotoxigenic E. coli and antibiotic resistance of the isolates from vegetables. Materials and methods: A total of 500 fresh vegetable samples were collected randomly from retail shops in Shahrekord, Iran. E. coli was isolated and identified using bacteriological and biochemical tests. PCR method was used to identify the rbfE, stx1, stx2 and eae genes. Also, antibiotic resistance of the isolates was determined by disk diffusion method. Results: The results represented that among 25 isolates possess virulence genes, 40, 12 and 4% of the isolates contained eaeA, STx2, and both genes, respectively. But none of them contained H7, STx1, and rfbE genes. The antibiotic resistance pattern demonstrated that the isolates were highly resistant to Gentamycin and cefotoxime. Discussion and conclusion: The results of this study showed that the presence of verotoxigenic E.coli in vegetables; and high resistance of the isolates to antibiotics could be hazardous for public health.

  19. 77 FR 73980 - Fresh Garlic From the People's Republic of China: Preliminary Results of Antidumping Duty...

    Science.gov (United States)

    2012-12-12

    ... DEPARTMENT OF COMMERCE International Trade Administration [A-570-831] Fresh Garlic From the People... order on fresh garlic from the People's Republic of China (PRC), covering the period of review (POR... merchandise at less than normal value (NV). The Department is also preliminarily determining that five...

  20. 78 FR 36168 - Fresh Garlic From the People's Republic of China: Final Results of Antidumping Duty...

    Science.gov (United States)

    2013-06-17

    ...- Market Economy Unit regarding ``Fresh Garlic from the People's Republic of China: Extension of Deadline...'s Republic of China: Final Results of Antidumping Duty Administrative Review; 2010-2011 AGENCY... administrative review of the antidumping duty order on fresh garlic from the People's Republic of China (PRC...

  1. Modelling quality of fresh-cut tomato based on stage of maturity and storage conditions

    NARCIS (Netherlands)

    Moreira Lana, M.

    2005-01-01

    Fresh-cut or minimally processed vegetables are those which have been trimmed and/or peeled and/or cut into 100% usable product and still maintain freshness. Contrary to other processing methods (freezing, canning and drying for example) the minimal processing operations reduce the shelf life in

  2. Climatic impacts of fresh water hosing under Last Glacial Maximum conditions: a multi-model study

    NARCIS (Netherlands)

    Kageyama, M.; Merkel, U.; Otto-Bliesner, B.; Prange, M.; Abe-Ouchi, A.; Lohmann, G.; Ohgaito, R.; Roche, D.M.V.A.P.; Singarayer, J

    2013-01-01

    Fresh water hosing simulations, in which a fresh water flux is imposed in the North Atlantic to force fluctuations of the Atlantic Meridional Overturning Circulation, have been routinely performed, first to study the climatic signature of different states of this circulation, then, under present or

  3. Modelling RGB colour aspects and translucency of fresh-cut tomatoes

    NARCIS (Netherlands)

    Moreira Lana, M.; Tijskens, L.M.M.; Kooten, van O.

    2006-01-01

    Translucency is one of the major problems in fresh-cut fruit. This phenomenon seriously limits the use of fruit by the fresh-cut industries. Techniques for measuring translucency in this kind of product are not readily available. As a consequence, the processes that are important in the development

  4. The effect of drying on the nutritional composition of fresh and dried ...

    African Journals Online (AJOL)

    A study was carried out to identify some nutritional properties of Moringa Oeifera leaf and to compare the nutritional qualities of fresh and dried leaves of Moringa Oleifera.Two samples each of fresh and dried Moringa Oeifera leaves were used for the experiment and the mean values in their nutritional qualities compared.

  5. 78 FR 41259 - Importation of Fresh Citrus Fruit From Uruguay, Including Citrus

    Science.gov (United States)

    2013-07-10

    ... therefore opposed importation of fresh citrus fruit from Uruguay until its effectiveness could be validated...'' imports. The commenter stated that this argument is invalid due to the year-round marketing of citrus... metric tons, which is less than 3 percent of U.S. production. Uruguay's total fresh orange and lemon...

  6. Fluorescence hyperspectral imaging technique for the foreign substance detection on fresh-cut lettuce

    Science.gov (United States)

    Nondestructive methods based on fluorescence hyperspectral imaging (HSI) techniques were developed in order to detect worms on fresh-cut lettuce. The optimal wavebands for detecting worms on fresh-cut lettuce were investigated using the one-way ANOVA analysis and correlation analysis. The worm detec...

  7. Accuracy, reliability, and timing of visual evaluations of decay in fresh-cut lettuce

    Science.gov (United States)

    Visual assessments are used for evaluating the quality of food products, such as fresh-cut lettuce packaged in bags with modified atmosphere. We have compared the accuracy and the reliability of visual evaluations of decay on fresh-cut lettuce performed with experienced and inexperienced raters. In ...

  8. The Effects Of Fresh And Thermoxidized Palm Oil Diets On Some ...

    African Journals Online (AJOL)

    The effects of fresh and thermoxidized palm oil diets on some haematalogical indices in the rat were investigated in albino rats (Wistar strain). The animals were divided into three groups namely, the first group fed on thermoxidized palm oil (TPO) diet, a second group fed on fresh palm oil (FPO) diet and a third fed on normal ...

  9. The USDA Fresh Fruit and Vegetable Program: A Case Study of Implementation and Consumption in Wisconsin

    Science.gov (United States)

    Jamelske, Eric M.; Bica, Lori A.

    2014-01-01

    Purpose/Objectives: The US Department of Agriculture created its Fresh Fruit and Vegetable Program (FFVP) to address poor nutrition and rising obesity among children. The FFVP allocates funding for selected elementary schools with at least 50% free/reduced price school meal enrollments to provide free fresh fruit and vegetable snacks to students.…

  10. Some mineral profiles of fresh and bottled palm wine – a ...

    African Journals Online (AJOL)

    Some mineral profiles of fresh palm wine and those of seven brands of bottled palm wine were analysed using atomic absorption spectroscopy and the values were compared. Three of the bottled samples contained toxic levels of either Pb or Cd or both. Neither metal was detected in fresh palm wine. Zn, Cr and Ni were 2 to ...

  11. Food safety standards in the fresh produce supply chain: advantages and disadvantages

    NARCIS (Netherlands)

    Uyttendaele, M.; Jacxsens, L.; Boxstael, Van S.; Kirezieva, K.; Luning, P.

    2015-01-01

    Abstract : Food safety standards in the fresh produce supply chain are discussed in view of the outcomes of a European Union Directorates General (EU DG) Research project FP7 Veg-i-Trade ‘Impact of climate change and international trade on the safety of fresh produce’. Various standards are outlined

  12. 9 CFR 327.21 - Inspection procedures for chilled fresh and frozen boneless manufacturing meat.

    Science.gov (United States)

    2010-01-01

    ... fresh and frozen boneless manufacturing meat. 327.21 Section 327.21 Animals and Animal Products FOOD... MEAT AND POULTRY PRODUCTS INSPECTION AND VOLUNTARY INSPECTION AND CERTIFICATION IMPORTED PRODUCTS § 327.21 Inspection procedures for chilled fresh and frozen boneless manufacturing meat. (a) Definitions...

  13. Consumer preferences for fresh citrus: Impacts of demographic and behavioral characteristics

    Science.gov (United States)

    From 2000 to 2006, per capita consumption of fresh citrus fruit increased by 11.0%, but the relative shares of types of citrus consumed changed. Per capita consumption of the historically dominant citrus fruit, fresh oranges, experienced a continuous decline from 12.4 pounds to 7.4 pounds from 1990 ...

  14. Effect of irradiation on fresh-keeping of strawberry stored at room temperature

    International Nuclear Information System (INIS)

    Zhao Yongfu; Xie Zongchuan; Lu Zhaoxin

    1999-01-01

    The fresh keeping period of strawberry irradiated with 4.0 kGy dose and stored at room temperature was prolonged to 6 days. Further experiment showed that the irradiation treatment decreased the number of mold in strawberry by two orders of magnitude, inhibited the strawberry fruit respiration and water loss, therefore, improved the effect of strawberry fresh-keeping

  15. The market value of freshness: observations from the swordfish and blue shark longline fishery

    NARCIS (Netherlands)

    Ishimura, G.; Bailey, M.L.

    2013-01-01

    One important component in determining the market value of fish is freshness, essentially the time period from capture to consumer. By shortening the time from harvest to landing, freshness can be improved and thus the market value may increase. The opportunistic nature of the marine capture

  16. Effect of essential oils against E. coli O157:H7 and Salmonella on fresh herbs

    Science.gov (United States)

    Consumer awareness of fresh herbs and demand has increased in recent years due to health benefits and distinct aroma in prepared food. There are specific markets for local growers, especially for organically grown herbs. Fresh herbs have been implicated in illnesses associated with Salmonella, E. co...

  17. A Liquid Desiccant Cycle for Dehumidification and Fresh Water Supply in Controlled Environment Agriculture

    KAUST Repository

    Lefers, Ryan

    2017-01-01

    of food import miles and capitalizing upon the demand for fresh, tasty, and nutritious food. However, the growing of food, both indoors and outdoors, consumes huge quantities of water - as much as 70-80% of global fresh water supplies. The utilization

  18. Effects of Chitosan-Essential Oil Coatings on Safety and Quality of Fresh Blueberries

    Science.gov (United States)

    Chitosan coating plus different essential oils was developed and applied to fresh blueberries, in order to find environmentally friendly and healthy treatments to preserve fresh fruit quality and safety during postharvest storage. Studies were first performed in vitro where wild-type Escherichia col...

  19. Does a frozen embryo transfer ameliorate the effect of elevated progesterone seen in fresh transfer cycles?

    Science.gov (United States)

    Healy, Mae Wu; Patounakis, George; Connell, Matt T; Devine, Kate; DeCherney, Alan H; Levy, Michael J; Hill, Micah J

    2016-01-01

    To compare the effect of progesterone (P) on the day of trigger in fresh assisted reproduction technology (ART) transfer cycles versus its effect on subsequent frozen embryo transfer (FET) cycles. Retrospective cohort study. Large private ART practice. Fresh autologous and FET cycles from 2011-2013. None. Live birth. A paired analysis of patients who underwent both a fresh transfer and subsequent FET cycle and an unpaired analysis of data from all fresh transfer cycles and all FET cycles were performed. We analyzed 1,216 paired and 4,124 unpaired cycles, and P was negatively associated with birth in fresh but not FET cycles in all analyses. Interaction testing of P and cycle type indicated P had a different association with birth in fresh versus FET cycles. When P was ≥ 2 ng/mL at the time of trigger, live birth was more likely in FET versus fresh cycles in the paired analysis (47% vs. 10%), in the unpaired analysis (51% vs. 14%), and in unpaired, good blastocyst only transfer subgroup (51% vs. 29%). Live birth was similar in FET cycles, with P ≥ 2 ng/mL versus P cycles, with P ≥ 2 ng/mL versus P cycle were negatively associated with live birth in the fresh transfer cycles but not in subsequent FET cycles. Freezing embryos and performing a subsequent FET cycle ameliorates the effect of elevated P on live-birth rates. Published by Elsevier Inc.

  20. Pre-Harvest light intensity affects shelf-Life of fresh-cut lettuce

    NARCIS (Netherlands)

    Witkowska, I.M.; Woltering, E.J.

    2010-01-01

    The industry of fresh-cut produce is continuously growing due to increasing demand for fresh, healthy and convenient foods. However, processing of vegetables accelerates quality deterioration due to structural, physiological and biochemical changes. Therefore, the value of the produce to the

  1. Fresh and Commercially Pasteurized Orange Juice: An Analysis of the Metabolism of Flavonoid Compounds

    Science.gov (United States)

    Orange juice is a rich source of flavonoids, mainly the flavanones hesperidin and narirutin, associated with health benefits in humans. The objective of this study was to analyze the uptake of flavonoids in humans after the consumption of two types of orange juice, fresh squeezed (fresh juice, FJ) a...

  2. The lycopene content in pulp ad peel of five fresh tomato varieties

    NARCIS (Netherlands)

    Markovic, K.; Panjkota-Krbavcic, I.; Krpan, M.; Bicanic, D.D.; Vahcic, N.

    2010-01-01

    The lycopene content in pulp and peel of five fresh tomato cultivars, most common on Croatian market, was determined by spectrophotometry and the high-performance liquid chromatography (HPLC). Peels from the raw tomatoes contained more lycopene (expressed on a fresh basis) than the pulps: the ratio

  3. 76 FR 5626 - Notice and Opportunity for Hearing: SinoFresh Healthcare, Inc.

    Science.gov (United States)

    2011-02-01

    ... SECURITIES AND EXCHANGE COMMISSION [Release No. 34-63776; File No. 0-49764] Notice and Opportunity for Hearing: SinoFresh Healthcare, Inc. January 26, 2011 Notice is hereby given that on November 1, 2010, SinoFresh Healthcare, Inc. (Applicant) filed with the Securities and Exchange Commission a Form...

  4. Fresh muscle fiber fragments on a scaffold in rats-a new concept in urogynecology?

    DEFF Research Database (Denmark)

    Boennelycke, Marie; Christensen, Lise; Nielsen, Lene F

    2011-01-01

    To investigate if a synthetic, biodegradable scaffold with either autologous in vitro cultured muscle-derived cells or autologous fresh muscle fiber fragments could be used for tissue repair.......To investigate if a synthetic, biodegradable scaffold with either autologous in vitro cultured muscle-derived cells or autologous fresh muscle fiber fragments could be used for tissue repair....

  5. Should we isolate human preantral follicles before or after cryopreservation of ovarian tissue?

    Science.gov (United States)

    Vanacker, Julie; Luyckx, Valérie; Amorim, Christiani; Dolmans, Marie-Madeleine; Van Langendonckt, Anne; Donnez, Jacques; Camboni, Alessandra

    2013-04-01

    To evaluate the survival and growth potential of human preantral follicles isolated before and after cryopreservation. Pilot study. Gynecology research unit in a university hospital. Six women aged 27 to 32 years. Six ovarian biopsy samples were cut into two equal parts, half subjected to slow-freezing followed by follicle isolation (cryo-iso group) and alginate-matrigel embedding, and half immediately processed for follicle isolation and alginate-matrigel embedding followed by slow-freezing (iso-cryo group) or used as fresh controls (fresh group). Follicle number, viability, diameter, and morphology. After 1,134 preantral follicles had been isolated from fresh biopsy samples and 1,132 from frozen specimens, the three groups were compared before and after 7 days of in vitro culture (IVC) in alginate-matrigel beads. No statistically significant differences in viability were found between the three groups before or after IVC, but follicle diameter increased in all three groups after IVC. Morphology analysis revealed well-preserved follicles in both the iso-cryo and cryo-iso groups after IVC. Human preantral follicles can be successfully cryopreserved before or after isolation without impairing their ability to survive and grow in vitro. This could lead to development of new protocols for follicle cryopreservation, IVC, and grafting in clinical and research settings for fertility preservation. Copyright © 2013 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  6. Study on Handing Process and Quality Degradation of Oil Palm Fresh Fruit Bunches (FFB)

    Science.gov (United States)

    Mat Sharif, Zainon Binti; Taib, Norhasnina Binti Mohd; Yusof, Mohd Sallehuddin Bin; Rahim, Mohammad Zulafif Bin; Tobi, Abdul Latif Bin Mohd; Othman, Mohd Syafiq Bin

    2017-05-01

    The main objective of this study is to determine the relationship between quality of oil palm fresh fruit bunches (FFB) and handling processes. The study employs exploratory and descriptive design, with quantitative approach and purposive sampling using self-administrated questionnaires, were obtained from 30 smallholder respondents from the Southern Region, Peninsular Malaysia. The study reveals that there was a convincing relationship between quality of oil palm fresh fruit bunches (FFB) and handling processes. The main handling process factors influencing quality of oil palm fresh fruit bunches (FFB) were harvesting activity and handling at the plantation area. As a result, it can be deduced that the handling process factors variable explains 82.80% of the variance that reflects the quality of oil palm fresh fruit bunches (FFB). The overall findings reveal that the handling process factors do play a significant role in the quality of oil palm fresh fruit bunches (FFB).

  7. Fresh market to supermarket: nutrition transition insights from Chiang Mai, Thailand.

    Science.gov (United States)

    Isaacs, Bronwyn Alison; Dixon, Jane; Banwell, Cathy

    2010-06-01

    A preliminary investigation into different eating patterns among Thai consumers who shop at fresh markets as opposed to supermarkets in Chiang Mai. A short questionnaire adopted from a previous study was administered to the forty-four participants, who comprised supermarket users, fresh market users and people who consistently shopped at both supermarkets and fresh markets. Participants were recruited within four fresh markets and two food courts attached to supermarkets in Chiang Mai. Chiang Mai residents who agreed to participate in the study. Equal numbers were regular fresh market and supermarket users. Initial results suggest an association between shopping at supermarkets and attributing bread with culinary value. Supermarkets may be potentially significant players in the 'nutrition transition', providing Thais more convenient shopping at some cost to their healthy food choices.

  8. Quality changes and nutrient retention in fresh-cut versus whole fruits during storage.

    Science.gov (United States)

    Gil, María I; Aguayo, Encarna; Kader, Adel A

    2006-06-14

    The influences of processing and storage on the quality indices and nutritional content of fresh-cut fruits were evaluated in comparison to whole fruits stored for the same duration but prepared on the day of sampling. Fresh-cut pineapples, mangoes, cantaloupes, watermelons, strawberries, and kiwifruits and whole fruits were stored for up to 9 days in air at 5 degrees C. The postcutting life based on visual appearance was shorter than 6 days for fresh-cut kiwifruit and shorter than 9 days for fresh-cut pineapple, cantaloupe, and strawberry. On the other hand, fresh-cut watermelon and mango pieces were still marketable after 9 days at 5 degrees C. Losses in vitamin C after 6 days at 5 degrees C were spoil before any significant nutrient loss occurs.

  9. Characterization and Evaluation of the Potential of a Diesel-Degrading Bacterial Consortium Isolated from Fresh Mangrove Sediment

    OpenAIRE

    Semboung Lang, Firmin; Destain, Jacqueline; Delvigne, Frank; Druart, Philippe; Ongena, Marc; Thonart, Philippe

    2016-01-01

    Hydrocarbons are ubiquitous and persistent organic pollutants in the environment. In wetlands and marine environments, particularly in mangrove ecosystems, their increase and significant accumulation result from human activities such as oil and gas exploration and exploitation operations. Remediation of these ecosystems requires the development of adequate and effective strategies. Natural attenuation, biostimulation, and bioaugmentation are all biological soil treatme...

  10. [Isolation of the cercaria Diplostomum phoxini (Faust, 1918) Arvy et Buttner, 1954 (Diplostomatidae) from fresh water mollusks of the Crimea].

    Science.gov (United States)

    Sten'ko, R P

    1976-01-01

    Data on the biology and morphology of cercariae of Diplostomum phoxini (Faust, 1918) Arvy et Buttner, 1954 are given. The cercariae were found in Radix auricularia from the middle part of the Burulcha river (Ukranian SSR, Crimea). In November, 1974 the invasion extensity of mollusks was 16.3%. No cercariae were found in spring and summer samples.

  11. Regulation of collagen production in freshly isolated cell populations from normal and cirrhotic rat liver: Effect of lactate

    International Nuclear Information System (INIS)

    Cerbon-Ambriz, J.; Cerbon-Solorzano, J.; Rojkind, M.

    1991-01-01

    Previous work has shown that lactic acid, and to a lesser extent pyruvic acid, is able to increase collagen synthesis significantly in liver slices of CCl4-treated rats but not normal rats. The purpose of this report is to document which cells in the cirrhotic liver are responsible for the lactate-stimulated increase in collagen synthesis. It was found that (a) incorporation of 3H-proline into protein-bound 3H-hydroxyproline is increased threefold to fourfold in hepatocytes from CCl4-treated rats as compared with normal rat hepatocytes; (b) neither the hepatocytes from normal nor those from CCl4-treated rats modify their collagen synthesizing capacity when 30 mmol/L lactic acid was added to the incubation medium; (c) nonparenchymal cells obtained from livers of CCl4-treated rats synthesize much less collagen than hepatocytes, but their synthesis is stimulated twofold by lactic acid; (d) from the different nonparenchymal cells, only fat-storing (Ito) cells increase collagen synthesis when lactic acid is present in the incubation medium. These results suggest that the increased lactic acid levels observed in patients with alcoholic hepatic cirrhosis may play an important role in the development of fibrosis by stimulating collagen production by fat-storing (Ito) cells

  12. The neuronal nitric oxide synthase inhibitor NANT blocks acetaminophen toxicity and protein nitration in freshly isolated hepatocytes.

    Science.gov (United States)

    Banerjee, Sudip; Melnyk, Stepan B; Krager, Kimberly J; Aykin-Burns, Nukhet; Letzig, Lynda G; James, Laura P; Hinson, Jack A

    2015-12-01

    3-Nitrotyrosine (3NT) in liver proteins of mice treated with hepatotoxic doses of acetaminophen (APAP) has been postulated to be causative in toxicity. Nitration is by a reactive nitrogen species formed from nitric oxide (NO). The source of the NO is unclear. iNOS knockout mice were previously found to be equally susceptible to APAP toxicity as wildtype mice and iNOS inhibitors did not decrease toxicity in mice or in hepatocytes. In this work we examined the potential role of nNOS in APAP toxicity in hepatocytes using the specific nNOS inhibitor NANT (10 µM)(N-[(4S)-4-amino-5-[(2-aminoethyl)amino]pentyl]-N'-nitroguanidinetris (trifluoroacetate)). Primary hepatocytes (1 million/ml) from male B6C3F1 mice were incubated with APAP (1mM). Cells were removed and assayed spectrofluorometrically for reactive nitrogen and oxygen species using diaminofluorescein (DAF) and Mitosox red, respectively. Cytotoxicity was determined by LDH release into media. Glutathione (GSH, GSSG), 3NT, GSNO, acetaminophen-cysteine adducts, NAD, and NADH were measured by HPLC. APAP significantly increased cytotoxicity at 1.5-3.0 h. The increase was blocked by NANT. NANT did not alter APAP mediated GSH depletion or acetaminophen-cysteine adducts in proteins which indicated that NANT did not inhibit metabolism. APAP significantly increased spectroflurometric evidence of reactive nitrogen and oxygen formation at 0.5 and 1.0 h, respectively, and increased 3NT and GSNO at 1.5-3.0 h. These increases were blocked by NANT. APAP dramatically increased NADH from 0.5-3.0 h and this increase was blocked by NANT. Also, APAP decreased the Oxygen Consumption Rate (OCR), decreased ATP production, and caused a loss of mitochondrial membrane potential, which were all blocked by NANT. Copyright © 2015 Elsevier Inc. All rights reserved.

  13. Mutation and premating isolation

    Science.gov (United States)

    Woodruff, R. C.; Thompson, J. N. Jr

    2002-01-01

    While premating isolation might be traceable to different genetic mechanisms in different species, evidence supports the idea that as few as one or two genes may often be sufficient to initiate isolation. Thus, new mutation can theoretically play a key role in the process. But it has long been thought that a new isolation mutation would fail, because there would be no other individuals for the isolation-mutation-carrier to mate with. We now realize that premeiotic mutations are very common and will yield a cluster of progeny carrying the same new mutant allele. In this paper, we discuss the evidence for genetically simple premating isolation barriers and the role that clusters of an isolation mutation may play in initiating allopatric, and even sympatric, species divisions.

  14. Physics in isolation

    International Nuclear Information System (INIS)

    Anon.

    1986-01-01

    In late May, about 330 physicists made their way up to isolated and beautiful Lake Louise high in the Canadian Rockies about 100 miles west of Calgary in a second effort to increase interactions between particle and nuclear physicists. The conference series aims to foster exciting and diverse physics by bringing the different physicists together somewhere which is so isolated that they must interact with each other. The formula worked very well and isolated Lake Louise was a huge success

  15. Physics in isolation

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    1986-09-15

    In late May, about 330 physicists made their way up to isolated and beautiful Lake Louise high in the Canadian Rockies about 100 miles west of Calgary in a second effort to increase interactions between particle and nuclear physicists. The conference series aims to foster exciting and diverse physics by bringing the different physicists together somewhere which is so isolated that they must interact with each other. The formula worked very well and isolated Lake Louise was a huge success.

  16. Morphometry and Morphology of Fresh Craters on Titan

    Science.gov (United States)

    Kirk, R. L.; Wood, C. A.; Neish, C.; Lucas, A.; Hayes, A. G.; Cassini Radar Team

    2011-12-01

    Cassini RADAR imagery obtained on Titan flyby T77 revealed a 40-km diameter fresh impact crater at 11.6° N 44.6° W. This is only the 8th crater identified with high confidence (Wood et al., 2010, Icarus 206, 334), and the 3rd (after Sinlap D=79 km and Ksa D=30 km) for which the depth can be estimated by comparing the foreshortening of the near and far walls. This "autostereo" technique yields an estimated depth of 680 m. The T77 image forms a stereo pair with the T17 discovery image of Ksa from which we estimate the depth of Ksa at 750-800 m, in close agreement with SARTopo data. The depth of Sinlap is 760 m based on SARTopo. Depth-diameter ratios for these craters thus range from 0.01 to 0.025 and the depths are comparable to but 200-400 m shallower than fresh craters of the same size on Ganymede (Bray et al., 2008, Met. Planet Sci. 43, 1979). The depth differences could be explained by initial crater morphometry, by relaxation in a different thermal environment, or (perhaps most plausibly given the bland floors of even the freshest Titan craters) to sedimentary infill. In contrast, the 18x36 km elliptical depression at Sotra Facula is much deeper than Ganymede craters of similar size (d=1500 m from stereo), supporting the conclusion that it is not an impact crater. All three craters exhibit a relatively radar-bright annulus around the outer edge of the floor, possibly as the result of mass wasting of blocky materials from the crater walls. The central part of each crater is darker. The central darker floor of the new crater is symmetrical and featureless, whereas Ksa has a bright central ring 7 km in diameter. Stereo spot heights indicate the ring is 350±100 m above the outer floor. This height is in close agreement with the scaling for Ganymede crater central peaks from Bray et al. (2008). The darker floor area of Sinlap is substantially asymmetrical with a small bright central spot whose elevation is unknown. The new crater has continuous, radar

  17. Who's on first? Part I: Influence of plant growth on C association with fresh soil minerals

    Science.gov (United States)

    Neurath, R.; Whitman, T.; Nico, P. S.; Pett-Ridge, J.; Firestone, M. K.

    2015-12-01

    Mineral surfaces provide sites for carbon stabilization in soils, protecting soil organic matter (SOM) from microbial degradation. SOM distributed across mineral surfaces is expected to be patchy and certain minerals undergo re-mineralization under dynamic soil conditions, such that soil minerals surfaces can range from fresh to thickly-coated with SOM. Our research investigates the intersection of microbiology and geochemistry, and aims to build a mechanistic understanding of plant-derived carbon (C) association with mineral surfaces and the factors that determine SOM fate in soil. Plants are the primary source of C in soil, with roots exuding low-molecular weight compounds during growth and contributing more complex litter compounds at senescence. We grew the annual grass, Avena barbata, (wild oat) in a 99 atom% 13CO2 atmosphere in soil microcosms incubated with three mineral types representing a spectrum of reactivity and surface area: quartz, kaolinite, and ferrihydrite. These minerals, isolated in mesh bags to exclude roots but not microorganisms, were extracted and analyzed for total C and 13C at multiple plant growth stages. At plant senescence, the quartz had the least mineral-bound C (0.40 mg-g-1) and ferrihydrite the most (0.78 mg-g-1). Ferrihydrite and kaolinite also accumulated more plant-derived C (3.0 and 3.1% 13C, respectively). The experiment was repeated with partially digested 13C-labled root litter to simulate litter decomposition during plant senescence. Thus, we are able evaluate contributions derived from living and dead root materials on soil minerals using FTIR and 13C-NMR. We find that mineral-associated C bears a distinct microbial signature, with soil microbes not only transforming SOM prior to mineral association, but also populating mineral surfaces over time. Our research shows that both soil mineralogy and the chemical character of plant-derived compounds are important controls of mineral protection of SOM.

  18. Anatomy and relations of the infraspinatus and the teres minor muscles: a fresh cadaver dissection study.

    Science.gov (United States)

    Bacle, Guillaume; Gregoire, Jean-Marc; Patat, Frédéric; Clavert, Philippe; de Pinieux, Gonzague; Laulan, Jacky; Lakhal, Walid; Favard, Luc

    2017-02-01

    Despite their functional importance, the infraspinatus (ISP) and teres minor (TM) muscles have been little investigated. This study aimed to describe the macroscopic morphology, innervation, and inter-relations of the ISP and TM muscles. Forty fresh cadaver dissections and histologic analysis were performed. Three groups of specimens were distinguished according to the rotator cuff tendon status: (1) intact rotator cuff; (2) supraspinatus tendon tears with intact ISP tendon; and (3) both supraspinatus and ISP tendons torn. Muscle fiber organization and muscle and tendon length were recorded. ISP and TM innervation and fiber structure were studied. ISP muscles were composed of three groups of fiber organized in two planes: two superficial groups, with mean pennation angles of, respectively, 27° ± 4° and 23° ± 3° with respect to the axis of the central tendon of the underlying group. TMs were thick fusiform muscles showing a parallel organization; 26 specimens (67 %) had aponeuroses isolating the TM, with a mean length of 5.2 ± 2.7 cm. Rotator cuff lesions were associated with relatively greater ISP tendon than muscle length. Innervation of the ISP muscle comprised 2-4 main branches from the suprascapular nerve and that of the TM 1 branch from the axillary nerve. ISP muscle body morphology derives from three groups of fibers in two planes. The TM has a parallel organization. Several nerve branches innervate the ISP muscle, whereas only one supplies the TM. The limits between the two muscles bodies consist of an aponeurotic fascia in two-thirds of cases.

  19. Repeated administration of fresh garlic increases memory retention in rats.

    Science.gov (United States)

    Haider, Saida; Naz, Nosheen; Khaliq, Saima; Perveen, Tahira; Haleem, Darakhshan J

    2008-12-01

    Garlic (Allium sativum) is regarded as both a food and a medicinal herb. Increasing attention has focused on the biological functions and health benefits of garlic as a potentially major dietary component. Chronic garlic administration has been shown to enhance memory function. Evidence also shows that garlic administration in rats affects brain serotonin (5-hydroxytryptamine [5-HT]) levels. 5-HT, a neurotransmitter involved in a number of physiological functions, is also known to enhance cognitive performance. The present study was designed to investigate the probable neurochemical mechanism responsible for the enhancement of memory following garlic administration. Sixteen adult locally bred male albino Wistar rats were divided into control (n = 8) and test (n = 8) groups. The test group was orally administered 250 mg/kg fresh garlic homogenate (FGH), while control animals received an equal amount of water daily for 21 days. Estimation of plasma free and total tryptophan (TRP) and whole brain TRP, 5-HT, and 5-hydroxyindole acetic acid (5-HIAA) was determined by high-performance liquid chromatography with electrochemical detection. For assessment of memory, a step-through passive avoidance paradigm (electric shock avoidance) was used. The results showed that the levels of plasma free TRP significantly increased (P < .01) and plasma total TRP significantly decreased (P < .01) in garlic-treated rats. Brain TRP, 5-HT, and 5-HIAA levels were also significantly increased following garlic administration. A significant improvement in memory function was exhibited by garlic-treated rats in the passive avoidance test. Increased brain 5-HT levels were associated with improved cognitive performance. The present results, therefore, demonstrate that the memory-enhancing effect of garlic may be associated with increased brain 5-HT metabolism in rats. The results further support the use of garlic as a food supplement for the enhancement of memory.

  20. Mobilities of radionuclides in fresh and fractured crystalline rock

    International Nuclear Information System (INIS)

    Torstenfelt, B.; Ittner, T.; Allard, B.; Andersson, K.; Olofsson, U.

    1982-12-01

    Sorption and migration of technetium, cesium and americium on fracture surfaces and fresh surfaces of granites taken from drilling cores from the Finnsjoen and Studsvik areas and the Stripa mine are reported. The three elements were used as reference elements with different chemistry and behaviour in water; under the conditions used in the experiments technetium exists as the heptavalent TcO -4 -ion, cesium as the non-complexed monovalent cation Cs + and americium as the strongly hydrolysed Am(OH)super (3-x) (x-1-4). The waters used were synthetic groundwaters representative of waters from the drilling holes. After the exposure of the fracture samples to spiked groundwater solutions for a period of three up to six months the penetration depths and concentration profiles were analysed and autoradiographs of cesium and americium distribution vs depth were taken. The sorption of technetium was found to be negligible. The transport of TcO -4 depends on accessibility to fractures and micro-fissures in the rock. Cesium is sorbed through an ion-exchange process. Migration of cesium depends not only on the transport in water into fractures and micro-fissures, but also on migration through mineral veins with a high CEC. Americium is strongly sorbed on most solid surfaces and did not migrate significantly during the contact time of three months. The diffusivity in granite was found to be around 10 - 13 m 2 /s for cesium; preliminary values for technetium and americium were 10 - 12 m 2 /s and less than 10 - 16 m 2 /s, respectively. (Authors)

  1. Evaluating oil palm fresh fruit bunch processing in Nigeria.

    Science.gov (United States)

    Anyaoha, Kelechi E; Sakrabani, Ruben; Patchigolla, Kumar; Mouazen, Abdul M

    2018-03-01

    Three routes of oil palm fresh fruit bunch (FFB) processing in Nigeria namely, industrial, small-scale and traditional were compared by means of determining fruit losses associated with each route. The fruits that are not recovered after each process were hand-picked and quantified in terms of crude palm oil (CPO), palm kernel (PK), mesocarp fibre (MF) and palm kernel shell (PKS). The energy value of empty fruit bunch (EFB), MF and PKS were used to determine the value of energy lost for each route. Additionally, the environmental implications of disposal of EFB were estimated, and socio-economics of the industrial and small-scale routes were related. The analysis showed that 29, 18, 75 and 27 kg of CPO, PK, MF and PKS were lost for every 1000 kg of FFB processed with the industrial route, whereas 5.6, 3.2, 1.4 and 5.1 g were lost with the small-scale route, respectively. Approximately 89 kWh and 31 kWh more energy were lost from MF and PKS with the industrial route than the other two routes, respectively. An equivalent of 6670 tonnes carbon dioxide equivalent of methane and nitrogen oxide was released due to the disposal of 29,000 tonnes of EFB from one palm oil mill. The monetary value of lost CPO per 1000 kg of FFB processed in the industrial route is more than the labour cost of processing 1000 kg of FFB in the small-scale route. The advantages of the industrial route are high throughput in terms of FFB processed per hour and high quality of CPO; however, high fruit loss is associated with it and therefore, the poorly threshed EFB is recommended to be fed into the small-scale route.

  2. Determination of trace metals in natural fresh waters

    International Nuclear Information System (INIS)

    Holm, K.; Borg, H.; Korhonen, M.

    1989-06-01

    The determination method still most widely used is atomic absorption spectrometry with graphite furnace. The natural levels of several elements are however too low to be accurately determined without any preconcentration. Besides, in sea water, the high salt content causes matric effects, which require time consuming separation steps as solvent extraction or ion exchange. The report describes two procedures for preconcentration of fresh water samples, freeze-drying and replicate injections in the furnace, respectively. The procedures are designed to be used on a routine basis. All water samples are collected in polypropylene bottles which are soaked before use in HCl 1+1, rinsed and allowed to stand until use filled with 0.1 M HNO 3 . The samples are preserved by addition of conc. HNO 3 (2 ml/l, sub boiling distilled). In the freeze-drying procedure, the samples are weighed and frozen in the pre-weighed polypropylene sampling bottles and evaporated to about one tenth of the original volume in the vaccum chamber of a freeze dryer. The samples are then weighed again for determination of the concentration factor and alayzed by graphite furnace AAS. When using the other procedure, the water samples are directly injected into the frunace for several times (2-8) before atomization and measurement of the absorption signal. The drying and ashing step is allowed to proceed after every injection. Comparisons of the two procedures have shown good agreement. The advantage of the replicate injection technique is primarily that the concentration factor is more esily controlled and repeated than by the freeze drying procedure. Further, the latter procedure sometimes suffers from precipitates being formed during the evaporation,especially in humic waters rich in iron. (12 figs., 7 tabs., 14 refs.)

  3. Consumer Acceptability of Fresh-Market Muscadine Grapes.

    Science.gov (United States)

    Brown, Kelly; Sims, Charles; Odabasi, Asli; Bartoshuk, Linda; Conner, Patrick; Gray, Dennis

    2016-10-14

    The objective of this research was to investigate the acceptability of muscadine grape (Vitis rotundifolia) genotypes (cultivars and selections) and to correlate overall liking to other quality measurements to determine the main drivers of liking. Twenty-two genotypes grown at the Univ. of Georgia-Tifton Campus were evaluated. Four retail commercial grape genotypes (Vitis vinifera and "Concord") were also evaluated for comparison. Panelists familiar with muscadine grapes used the hedonic general labeled magnitude scale (HgLMS, -100 = strongest disliking of any kind ever experienced, +100 = strongest liking of any kind ever experienced) to rate overall liking and the liking of appearance, flavor, pulp texture, and skin texture. Puncture testing was done to assess grape berry texture, and compositional attributes soluble solids and pH were also measured. The sensory results indicated that the grapes were variable with overall liking scores from 12.2 to 39.6. The factors highly correlated with overall liking scores were muscadine flavor, pulp and skin liking, while a significant negative correlation was found between skin liking and skin texture and mechanical texture measures. The muscadine grapes with the highest overall liking scores were Ga. 5-1-34 and Ga. 2-8-21. Principal component analysis confirmed that grapes with a thinner skin and a higher pH tended to group around overall liking and flavor points. These results indicate that even among panelists familiar with muscadine grapes, skin thickness is a negative characteristic. Breeding for thinner skins may be a positive step in muscadines gaining a more widespread appeal in the fresh fruit market. © 2016 Institute of Food Technologists®.

  4. Taste and aroma of fresh and stored mandarins.

    Science.gov (United States)

    Tietel, Zipora; Plotto, Anne; Fallik, Elazar; Lewinsohn, Efraim; Porat, Ron

    2011-01-15

    During the last decade there has been a continuous rise in consumption of fresh easy-to-peel mandarins. However, mandarins are much more perishable than other citrus fruit, mainly due to rapid deterioration in sensory acceptability after harvest. In the current review we discuss the biochemical components involved in forming the unique flavor of mandarins, and how postharvest storage operations influence taste and aroma and consequently consumer sensory acceptability. What we perceive as mandarin flavor is actually the combination of basic taste, aroma and mouth-feel. The taste of mandarins is principally governed by the levels of sugars and acids in the juice sacs and the relative ratios among them, whereas the aroma of mandarins is derived from a mixture of different aroma volatiles, including alcohols, aldehydes, ketones, terpenes/hydrocarbons and esters. During postharvest storage and marketing there is a gradual decrease in mandarin sensory acceptability, which has been attributed to decreases in acidity and typical mandarin flavor, paralleling an accumulation of off-flavor. Biochemical analysis of volatile and non-volatile constituents in mandarin juice demonstrated that these changes in sensory acceptability were concomitant with decreases in acidity and content of terpenes and aldehydes, which provide green, piney and citrus aroma on the one hand, and increases in ethanol fermentation metabolism products and esters on the other, which are likely to cause 'overripe' and off-flavors. Overall, we demonstrate the vast importance of the genetic background, maturity stage at harvest, commercial postharvest operation treatments, including curing, degreening and waxing, and storage duration on mandarin sensory quality. Copyright © 2010 Society of Chemical Industry.

  5. Trends of fresh green food for lactating roe deer females

    Science.gov (United States)

    Menzel, Annette; Stahl, Benjamin; Laube, Julia

    2017-04-01

    Increasing temperatures, changed precipitation patterns as well as more intense and frequent extreme events will alter the phenology of both flora and fauna and shift species distributions. Moreover, farmers respond to climate change by adapting land use and management, and thus the cultural landscape is changing. Therefore, the health and fitness of wild animals will be largely affected by factors directly and indirectly linked to climate change. Familiar examples of mismatch due to loss of temporal synchrony in food webs are known from birds (timing of migration or egg laying in relation to food resources) and insect pollination (timing of first flights in relation to plant flowering). However, also large herbivory mammals may suffer from climate change induced phenological mismatch if they are not able to "surf on the green wave" any more. Taking roe deer (Capreolus capreolus L.) as key example, we studied changes in the spring phenology of potential food plants during the last four decades in southern Germany. Our analysis is based on the phenological observations of the German Meteorological Service as well as on the comprehensive multi-species dataset of a dedicated citizen scientist. Roe deer is sensitive to slight phenological changes of food plants, since only the first fresh green contains maximal protein contents which are needed by the females to suckle their fawns born mid of May till mid of June. We find indications for an increasing number of food plant species available in the lactation period, however probably with a decreasing food quality over the decades. Since females have delayed implantation it may be difficult to well synchronise the postnatal period to the vegetation development. A unique dataset of marked fawns suggests that also the timing of birth has slightly advanced in recent decades. We discuss these changes in the match-mismatch of lactation period of roe deer and spring leaf phenology and their driving factors in detail.

  6. Purification of papain from fresh latex of Carica papaya

    Directory of Open Access Journals (Sweden)

    Rubens Monti

    2000-01-01

    Full Text Available In the present study we wish to report a method of crystallizing papain from fresh papaya latex which gave higher yields than previously reported. This method does not involve the use of sulphydryl reagents. The papain thus obtained is practically pure and shows a single band when submitted to electrophoresis on polyacrylamide gel, and is identical to the papain obtained by other methods. In routine enzymatic assays, specific activity was measured using Z-gly-pNP and BAEE as substrates. Papain crystallized by this method, without the use of high concentrations of salts or thiol-containing substances such as cysteine and dithiothreitol, is obtained in the form of a complex with natural inhibitors existent in latex which can be removed by dialysis.No presente trabalho apresenta-se um método de cristalização da papaína oriunda do látex fresco de mamão, o qual apresenta uma alta produtividade em relação aos métodos previamente descritos. A metodologia aqui descrita não envolve o uso de reagentes sulfidrílicos, a papaína foi obtida de forma praticamente pura, apresentando uma simples banda quando submetida a eletroforese, e com propriedades idênticas àquelas obtidas por outros métodos. A atividade específica foi determinada utilizando Z-gly-pNP e BAEE como substrato. A papaína obtida por essa metodologia, sem uso de substâncias tais como cisteína e ditiotreitol, apresenta-se na forma de um complexo com inibidores naturais, os quais podem ser removidos por diálise.

  7. Lesion Size Does Not Predict Outcomes in Fresh Osteochondral Allograft Transplantation.

    Science.gov (United States)

    Tírico, Luis E P; McCauley, Julie C; Pulido, Pamela A; Bugbee, William D

    2018-03-01

    Cartilage repair algorithms use lesion size to choose surgical techniques when selecting a cartilage repair procedure. The association of fresh osteochondral allograft (OCA) size with graft survivorship and subjective patient outcomes is still unknown. To determine if lesion size (absolute or relative) affects outcomes after OCA transplantation. Cohort study; Level of evidence, 3. The study included 156 knees in 143 patients who underwent OCA transplantation from 1998 to 2014 for isolated femoral condyle lesions. The mean age was 29.6 ± 11.4 years, and 62.9% were male. The majority of patients (62.2%) presented for cartilage repair because of osteochondritis dissecans. The mean graft area, used as a surrogate for absolute size of the lesion, was 6.4 cm 2 (range, 2.3-11.5 cm 2 ). The relative size of the lesion was calculated as the tibial width ratio (TWR; ratio of graft area to tibial width) and affected femoral condyle ratio (AFCR; ratio of graft area to affected femoral condyle width) using preoperative radiographs. All patients had a minimum follow-up of 2 years. Further surgical procedures were documented, and graft failure was defined as revision OCA transplantation or conversion to arthroplasty. International Knee Documentation Committee (IKDC) pain, function, and total scores were obtained. Satisfaction with OCA transplantation was assessed. The mean follow-up among patients with grafts remaining in situ was 6.0 years (range, 1.9-16.5 years). The OCA failure rate was 5.8%. Overall survivorship of the graft was 97.2% at 5 years and 93.5% at 10 years. No difference in postoperative outcomes between groups was found in absolute or relative size. Change in IKDC scores (from preoperative to latest follow-up) was greater for knees with large lesions compared to knees with small lesions, among all measurement methods. Overall satisfaction with the results of OCA transplantation was 89.8%. The size of the lesion, either absolute or relative, does not influence

  8. PRESENCE OF FUNGAL CONTAMINATION IN THE PRODUCTION CHAIN OF FRESH FILLED PASTA, WITH PARTICULAR REGARD TO THE PENICILLIUM GENUS

    Directory of Open Access Journals (Sweden)

    S. Lomonaco

    2011-08-01

    Full Text Available A shelf-life study was carried out on fresh filled pasta in order to evaluate the presence of fungal contamination in the production process (environment, raw materials and finished products, with particular regard to the Penicillium genus. Analyses were conducted on three types of products, while air sampling was carried out in shaping processes and packaging areas. After isolation of molds, phenotypic identification of Penicillium genus and genotyping confirmation were carried out. As for the isolated strains, the species identified are those expected from this type of matrix: 40.7% P. viridicatum, 14.8% P. griseofulvum, 11.1% P. chrysogenum, 7.4% for P. citrinum, P. nalgiovense and P. crustosum and finally 3.7% for each of the three remaining species (P. roqueforti, P. formosanum, P. atramentosum. Results showed environmental contamination, particularly at the end of the work day. In fact, 59% of identified Penicillia originated from air samples, while only 10% from foods. Moreover, even if fungal contamination was observed at all considered times, level of contamination were generally low and never resulted in spoilage of the product as no macroscopically visible colonies were observed. It is therefore clear that pasteurization and modified atmosphere packaging are able to control the growth of potentially present fungal contamination.

  9. [Contamination of protozoa by enteroviruses in fresh water and sewages].

    Science.gov (United States)

    Skachkov, M V; Al'misheva, A Sh; Plotnikov, A O; Nemtseva, N V; Skvortsov, V O

    2009-01-01

    To determine rate of infection of protozoa by enteroviruses to assess the potential role of protozoa as a natural reservoir of enteroviruses. The samples were collected from flowing and stagnant water reservoirs in Orenburg region in summer and autumn. The samples of sewages were taken in all stages of their treatment. Cultures of protozoa were isolated with micromanipulator equipped with micropipette, incubated on Pratt's medium at 25 degrees C and fed with Pseudomonas fluorescens culture. RNA of enteroviruses was detected by reverse transcription polymerase chain reaction (RT-PCR). Seventy-two protozoan species were found in Ural river, whereas 15 and 38 species were found in lakes and sewages respectively. Enteroviruses were detected by RT-PCR in 61.8% cultures of protozoa belonging to 23 species of flagellates, amoebae and ciliates isolated from natural water bodies undergoing anthropogenic impact as well as from sewages in all stages of their treatment. Predominant localization of enteroviruses in dominant taxons of protozoa (Paraphysomonas sp., Spumella sp., Petalomonas poosilla, Amoeba sp.) was noted. Obtained data confirm presence of enteroviruses in protozoa living both in flowing and stagnant recreation natural water bodies as well as in sewages and confirm the hypothesis of persistence of enteroviruses in protozoa and the reservoir role of the latter. Contingency of life cycles of viruses and protozoa allows to explain the seasonality of aseptic meningitis incidence caused by enteroviruses, which peaks in summer and autumn when protozoa massively multiply in water bodies.

  10. The Inhibitor Pathogen Bacteria’s of Sea Grape Caulerpa lentillifera Applies on Fresh Fish

    Directory of Open Access Journals (Sweden)

    Alfonsina M.Tapotubun

    2016-12-01

    Full Text Available Contamination of pathogen bacteries at the fresh fish may occur during the post harvesting to the consuming period, and endanger human health. One of simple and safe way to protect secureness of fresh fish food is the use of Caulerpa lentillifera to push down pathogen bacteries activity. The aims of this research to investigate lability of sea grape (Caulerpa lentillifera against the activity of pathogen bacteries Escherichia coli, Vibrio cholerae and Salmonella sp., in fresh fish, during storage phase, at ambient and ice temperatures. Method used in this research is experimental laboratories method, that is, 10%, 20% and 30% blended sea grapes applied on fresh fishes, Selar crumenopthalmus during storage of 1, 2 and 3 days at ambient and ice temperatures. All the applied of blended of Caulerpa lentillifera, shows the ability to obstruct the activity of bactery group coliform and Escherichia coli on fresh fish Selar crumenopthalmus up to 2x 24 hours, at ambient temperature, and 3x24 hours at ice temperature. During storage period, the occurance and grows of Salmonella sp. and Vibrio cholerae bacteries is undetected. Fresh sea grapes concentration of 10% is sufficient to be applied on fresh fish to obstruct the activity of pathogen bacteries Escherichia coli during storage time of 2 x 24 hours at room temperature, and 3 x 24 hours at ice temperature, and to block the occurance of Vibrio cholerae and Salmonella sp. during storage period. .

  11. DETERMINATION OF MINERAL CONTAIN AND BACTERIA CONTAMINANT ON ORGANIC AND NONORGANIC FRESH VEGETABLES

    Directory of Open Access Journals (Sweden)

    Harsojo Harsojo

    2010-06-01

    Full Text Available The determination of mineral content and bacteria contaminant on fresh vegetable of long bean (Vegan ungulate Wall., white cabbage (Basic tolerance L., and lettuce (Lectuca sativa L. that cultivated by organic and nonorganic system have been done. The mineral content has been analyzed using neutron activation analysis and atomic absorption spectroscopy method, while bacteria contaminant by total plate count number using Nutrient Agar, Mac Conkey Agar, Baird Parker medium, and Salmonella using selective medium. The results showed that there are some essential mineral such as Fe, Zn, Ca, Co, and nonessential mineral Cd. There is tendency that fresh vegetable that cultivated by organic system contained Fe, Zn, Ca, Co and Cd mineral less than nonorganic. The Zn mineral content in nonorganic of fresh vegetable were higher than the limit of threshold number from Health Department, Republic of Indonesia (2004, while Cd mineral in organic or nonorganic of fresh vegetable were greater then threshold number from Codex Alimentarius Commision. The measurement of bacteria contaminant on organic and nonorganic of fresh vegetables contained aerob, coli, and Staphylococcus bacteria in organic of fresh vegetables were less compared to nonorganic of fresh vegetables.   Keywords: mineral, bacteria aerob, coli, Staphylococcus, Salmonella, organic, and nonorganic vegetable, neutron activation

  12. Isolated optic nerve pseudotumour

    International Nuclear Information System (INIS)

    Patankar, T.; Prasad, S.; Krishnan, A.; Laxminarayan, R.

    2000-01-01

    Isolated optic nerve involvement by the idiopathic inflammatory process is a rare finding and very few reports are available. Here a case of an isolated optic nerve inflammatory pseudotumour presenting with gradually progressive unilateral loss of vision is described. It showed dramatic response to a trial of steroids and its differential diagnoses are discussed. Copyright (1999) Blackwell Science Pty Ltd

  13. Superbugs in the supermarket? Assessing the rate of contamination with third-generation cephalosporin-resistant gram-negative bacteria in fresh Australian pork and chicken.

    Science.gov (United States)

    McLellan, Jade E; Pitcher, Joshua I; Ballard, Susan A; Grabsch, Elizabeth A; Bell, Jan M; Barton, Mary; Grayson, M Lindsay

    2018-01-01

    Antibiotic misuse in food-producing animals is potentially associated with human acquisition of multidrug-resistant (MDR; resistance to ≥ 3 drug classes) bacteria via the food chain. We aimed to determine if MDR Gram-negative (GNB) organisms are present in fresh Australian chicken and pork products. We sampled raw, chicken drumsticks (CD) and pork ribs (PR) from 30 local supermarkets/butchers across Melbourne on two occasions. Specimens were sub-cultured onto selective media for third-generation cephalosporin-resistant (3GCR) GNBs, with species identification and antibiotic susceptibility determined for all unique colonies. Isolates were assessed by PCR for SHV, TEM, CTX-M, AmpC and carbapenemase genes (encoding IMP, VIM, KPC, OXA-48, NDM). From 120 specimens (60 CD, 60 PR), 112 (93%) grew a 3GCR-GNB ( n  = 164 isolates; 86 CD, 78 PR); common species were Acinetobacter baumannii (37%), Pseudomonas aeruginosa (13%) and Serratia fonticola (12%), but only one E. coli isolate. Fifty-nine (36%) had evidence of 3GCR alone, 93/163 (57%) displayed 3GCR plus resistance to one additional antibiotic class, and 9/163 (6%) were 3GCR plus resistance to two additional classes. Of 158 DNA specimens, all were negative for ESBL/carbapenemase genes, except 23 (15%) which were positive for AmpC, with 22/23 considered to be inherently chromosomal, but the sole E. coli isolate contained a plasmid-mediated CMY-2 AmpC. We found low rates of MDR-GNBs in Australian chicken and pork meat, but potential 3GCR-GNBs are common (93% specimens). Testing programs that only assess for E. coli are likely to severely underestimate the diversity of 3GCR organisms in fresh meat.

  14. Experimental research on fresh mussel meat irradiated by high-dose electron beam

    International Nuclear Information System (INIS)

    Xiao Lin; Lu Ruifeng; Hu Huachao; Wang Chaoqi; Liu Yanna

    2011-01-01

    The sterilization storage of fresh mussel irradiated high-dose electron beam was studied. From the subjective assessment by the weighted average of the test and other determined parameters, it can be concluded that the flavor of fresh mussel meat sealed canned food irradiated by high-dose electron beam has not been significant affected, and various micro-organisms can be killed effectively, which means that the irradiated fresh mussel meat can be preserved for long-term at room temperature. Therefore the method might resolve the problems induced by traditional frozen preservation methods. (authors)

  15. A comprehensive approach to evaluate the freshness of strawberries and carrots

    DEFF Research Database (Denmark)

    Peneau, S.; Brockhoff, Per B.; Escher, F.

    2007-01-01

    of strawberries and carrots that varied in cultivar, as well as with time and conditions of storage. Product characteristics measured by descriptive sensory and physico-chemical analyses were related to consumer and expert panel (individual and consensus) ratings of freshness. Results showed that a large number...... of attributes contributed to the freshness of strawberries and carrots and that those were also indicators of the physiological ageing of these products. Our results suggest that consumer evaluation of fruit and vegetable freshness corresponds to an evaluation of this ageing process through the observation...

  16. Effect of gamma irradiation on curcuminoids and volatile oils of fresh turmeric ( Curcuma longa)

    Science.gov (United States)

    Dhanya, R.; Mishra, B. B.; Khaleel, K. M.

    2011-11-01

    In our earlier study a radiation dose of 5 kGy was reported to be suitable for microbial decontamination and shelf life extension of fresh turmeric ( Curcuma longa), while maintaining its quality attributes. In continuation of that work, the effect of gamma radiation on curcuminoids and volatile oil constituents in fresh turmeric was studied. Fresh peeled turmeric rhizomes were gamma irradiated at doses of 1, 3 and 5 kGy. Curcuminoid content and volatile oils were analyzed by reverse phase HPLC and GC-MS, respectively. The curcuminoid content was slightly increased by gamma irradiation. No statistically significant changes were observed due to irradiation in majority of the volatile oil constituents.

  17. Identifikasi Penyakit Aeromonad pada Budi Daya Ikan Air Tawar di Bali (IDENTIFICATION OF AEROMONAD DISEASE IN FRESH WATER AQUACULTURE IN DENPASAR, BALI

    Directory of Open Access Journals (Sweden)

    Surya Amanu

    2015-05-01

    Full Text Available Fresh water and marine fish horticulture in Bali is often harmed by the outbreak of diseases such asthose caused by Aeromonas sp (aeromonad disease.Aims ofstudy were 1 to find out the primary agent ofthe aeomonad disease in the fresh water aquaculture in Bali based on conventional and molecularidentification, 2 to find out the genetic variability of Aeromonas species, 3 to determine the effectiveantibiotic against the agent. Samples of fishes were collected from 5 different locations of fresh wateraquaculture that had high number of morbidity and mortality. Many different fishes which showed clinicalsign such as skin lesion and exophthalmus were collected.Aeromonas hydrophila and A. salmonicida wereisolated and identified from fishes, they were then identified molecularly with DNA extraction, DNAamplification in 16S rRNA gene, purification and sequencing. Sequences of both Aeromonas species fromdifferent location were analysed to create the phylogenetic tree with Maximum Parsimony and NeighborJoining method. Sensitivity of 5 antibiotics to both species of Aeromonas were done to determine the bestantibiotic against the disease. Aeromonad disease were found only in 3 regions in Bali. As many as 10isolates of A.salmonicida and 11 isolates of A.hydrophila were examined. The histopathological examinationshowed dermatitis, epicarditis, retinitis, liver and kidney congestion in fish.There were two clusters ofA.salmonicida, subspecies smithiaand subspecies achromogenes.Aeromomas hydrophyla had a close relationwith A. veronii.Aeromonas salmonicida subspecies salmonicida has not been found in Bali. Enrofloxacineand gentamycin was the best antibiotic for treating the Aeromonad disease which were more effective ascompared to3 other antibiot ics (Ampicillin, Doxycycline, and Eritromycin.

  18. Use of radiosensitivity to identify irradiated fresh poultry products

    International Nuclear Information System (INIS)

    Copin, M.P.; Bourgeois, C.M.

    1991-01-01

    Microbiological comparison between irradiated and non-irradiated foodstuff has been studied for a long time as a way to detect whether a foodstuff has been irradiated or not. Generally, the proposed methods are based on the fact that ionization select species of bacteria which are recognized to be radioresistant. So reduction or elimination of known radiation sensitive microbes from the normal endogenous microflora could give an indication that the foodstuff has been irradiated, predominance of known radioresistant bacteria should be another indication. In the present work, we try to develop a test based on the radiosensitivity of the bacteria independently of their place. These first experiments show that the determination of radiosensitivity of strains isolated from a product or even of global radioresistance of mesophilic microflora could indicate if this product has been previously submitted to ionizing radiations. (4 tabs)

  19. Effect of a bacteriophage cocktail in combination with modified atmosphere packaging in controlling Listeria monocytogenes on fresh-cut spinach

    Directory of Open Access Journals (Sweden)

    Boyacioglu O.

    2016-06-01

    Full Text Available A Listeria monocytogenes-specific bacteriophage cocktail was evaluated for its activity against a nalidixic acid-resistant L. monocytogenes (Lm-NalR isolate on fresh-cut spinach stored under modified atmosphere packaging at various temperatures. Pieces (~2 × 2 cm2 of fresh spinach inoculated with 4.5 log CFU/cm2 Lm-NalR were sprayed with the phage cocktail (6.5 log plaque-forming units [PFU]/cm2 or a control. The samples were stored at 4°C or 10°C for up to 14 d in sealed packages filled with either atmospheric air (AA or modified atmosphere (MA. At 4°C under AA, the phages significantly (P ≤ 0.05 lowered the Lm-NalR populations on spinach, compared to control-treated inoculated samples, by 1.12 and 1.51 log CFU/cm2 after 1 and 14 d, respectively. At 4°C under MA, Lm-NalR was significantly reduced by 1.95 log CFU/cm2 compared to control leaves after both 1 and 14 d. At 10°C under AA, the phages significantly reduced Lm-NalR by 1.50 and 2.51 log CFU/cm2 after 1 and 14 d compared to the control. Again at 10°C under MA, the phages significantly reduced Lm-NalR by 1.71 and 3.24 log CFU/cm2 compared to control after 1 and 14 d, respectively. The results support the potential of lytic bacteriophages in effectively reducing populations of L. monocytogenes on freshcut leafy produce, under both AA and MA conditions.

  20. 75 FR 38778 - Fresh Garlic from the People's Republic of China: Extension of Time Limit for the Final Results...

    Science.gov (United States)

    2010-07-06

    ... DEPARTMENT OF COMMERCE International Trade Administration [A-570-831] Fresh Garlic from the People... (the Department) issued the preliminary results of the new shipper review of fresh garlic from the... the period of review of November 1, 2008 through April 30, 2009. See Fresh Garlic from the People's...