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Sample records for satheesh sk moorthy

  1. K. Sridhar Moorthy's Theoretical Modelling in Marketing - A Review ...

    African Journals Online (AJOL)

    K. Sridhar Moorthy's Theoretical Modelling in Marketing - A Review. ... Modelling has become a visible tool in many disciplines including marketing and several marketing models have ... EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  2. COVERING RACE AND RELIGION: THE MOORTHY AND NYONYA TAHIR CASES IN FOUR MALAYSIAN NEWSPAPERS

    Directory of Open Access Journals (Sweden)

    Ngu Teck Hua

    2006-01-01

    Full Text Available Reporting on issues like race and religion in a multi-racial and multi-religious society is not an easy media responsibility. In a country like Malaysia where racial and religious sensitivities abound, the media have to constantly tread on precarious ground, balancing between what to write and how to write it. Much of the concern over the reporting of sensitive issues stems from the belief that a wrong move may have dire consequences, as seen in the recent controversy and furore over the Prophet Muhammad caricature published in the Danish newspaper, Jyllands-Posten.This study analyses two recent racial/religious issues – M. Moorthy and Nyonya Tahir cases – as reported in the New Straits Times (NST, Utusan Malaysia (UM, Malaysia Nanban (MN and Sin Chew Daily (SCD. The former case caused a stir when there was a tussle between Moorthy’s Indian/Hindu family and the Federal Territory Islamic Affairs Council which argued that Moorthy had converted to Islam when he was alive without the family’s knowledge. The latter was a case of a Malay/Muslim woman who, while alive, had denounced Islam and lived as a Chinese practising Buddhism.This study analyses how the two race and religion-related controversial issues were treated in the various language newspapers in Malaysia. A preliminary finding showed that, true to the communal nature of the Malaysian press, there was an apparent slant in how the ethnic press covered these two issues. For instance, in terms of prominence given to the stories, SCD, while downplaying the Moorthy story, dedicated more space to the Nyonya Tahir case. Similarly, MN highlighted the Moorthy story and downplayed the Nyonya Tahir case. The different newspapers were also seen to ''favour'' the subject they covered according to the ethnicity.

  3. Minority game with SK interactions

    International Nuclear Information System (INIS)

    Menezes, Pedro Castro; Sherrington, David

    2013-01-01

    A batch minority game with fake random history and additional SK-like quenched interaction is introduced and analysed. A mixing parameter λ quantifies the admixture and dictates the relative dominance of the two contributions: if λ → 0, agent decisions are based on their strategies and point-scores alone, as in the pure minority game, whereas for λ > 0 the agents also communicate with each other directly and update their points accordingly. Keeping the minority game dynamics in which the agents’ points are updated in parallel at each time step, the aim is to understand what happens if instead of simply using the normal strategy-based decisions, the agents also take account of an ‘effective field’ generated by the other agents. It is shown that the SK interaction introduces a ‘noise’ term which is broader than that in the normal minority game and which furthermore kills the normal phase transition. It is also shown that the same effect would occur if, instead of an SK interaction, Gaussian-distributed quenched random fields are added. By calculating order parameters in the time-translational invariant phase we show that the system is persistent in a ergodic phase. Both simulational and analytical results are presented. (paper)

  4. Journal of Earth System Science | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Earth System Science. P Pant. Articles written in Journal of Earth System Science. Volume 117 Issue S1 July 2008 pp 303-313. Characteristics of spectral aerosol optical depths over India during ICARB · S Naseema Beegum K Krishna Moorthy Vijayakumar S Nair S Suresh Babu S K Satheesh V ...

  5. Journal of Earth System Science | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Earth System Science. C B S Dutt. Articles written in Journal of Earth System Science. Volume 117 Issue S1 July 2008 pp 243-262. Integrated Campaign for Aerosols, gases and Radiation Budget (ICARB): An overview · K Krishna Moorthy S K Satheesh S Suresh Babu C B S Dutt · More Details ...

  6. Pharmacological modulation of SK3 channels

    DEFF Research Database (Denmark)

    Grunnet, M; Jespersen, Thomas; Angelo, K

    2001-01-01

    Small-conductance, calcium-activated K+ channels (SK channels) are voltage-insensitive channels that have been identified molecularly within the last few years. As SK channels play a fundamental role in most excitable cells and participate in afterhyperpolarization (AHP) and spike-frequency adapt...... at concentrations of 3 microM and above. Amitriptyline, a tricyclic antidepressive widely used clinically, inhibits SK3 channels with an IC50 of 39.1 +/- 10 microM (n=6)....

  7. Skærmens samfund/Screen Society

    DEFF Research Database (Denmark)

    Fausing, Bent

    Udgivelsen skal komme dels i en danks udgave og i en engelsk oversættelse. Der er skærme overalt i samfundet. Det er vanskeligere at finde et rum uden end et med en skærm. Hvad betyder det, at vi får oplevelser via skærme? Hvordan er skærmens æstetik og dens visualiseringer? Hvordan interagerer vi...... med skærme? Grænser skaber energi lærte vi i skolen, hvilke energier samles inden for skærme?...

  8. Formation of Skål International Club in Vietnam

    OpenAIRE

    Tran, Quang

    2014-01-01

    This paper presents a project plan of Skål International Vietnam as a new joining club, with the purpose of forming a new Skål International club in Vietnam. The idea was born in September, 2011 during the 72nd Skål International World Congress in Turku, Finland, where the author – a Young Skål had invited to attend . Vietnam situated in the peninsula of South-East Asia, the country holds great potentials of nature, culture and human power for tourism growth. In spite of joining an Inter...

  9. Elektrooniline maksekäsk kiirendab kohtumenetlust / Marko Aavik

    Index Scriptorium Estoniae

    Aavik, Marko, 1977-

    2008-01-01

    Eesti kohtusüsteemist ning maksekäsumenetlusest. Ilmunud ka Valgamaalane 17. juuni 2008, lk. 2 ; Harju Ekspress 20. juuni 2008, lk. 6 ; Meie Maa 2. juuli 2008, lk. 5 ; Nädaline 1. juuli 2008, lk. 5 ; Severnoje Poberezhje 2. juuli 2008, lk. 2 (Melnitsõ pravosudija stali molot bõstreje) ; Sakala 18. juuli 2008, lk. 2 ; Vooremaa : Jõgeva Valla Teataja 4. okt. 2008, lk. 2 ; Meie Maa 8. okt. 2008, lk. 6, pealkiri kujul: e-maksekäsk lahendab võlanõude kiiresti ; Vooremaa 7. okt. 2008, lk. 2, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Koit 9. okt. 2008, lk. 3, pealkiri kujul: e-maksekäsk säästab aega ja raha ; Virumaa Teataja 9. okt. 2008, lk. 7, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Kuulutaja 10. okt. 2008, lk. 4, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Nädaline 14. okt. 2008, lk. 10, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Lääne Elu 14. okt. 2008, lk. 5, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Pärnu Postimees 18. okt. 2008, lk. 19, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti

  10. Enviroportal.sk - information portal about the environment

    International Nuclear Information System (INIS)

    Navratil, R.; Kmet, M.

    2005-01-01

    In this presentation author deals with history, conception and exploitation of information portal about environment in the Slovak Republic. This portal - Enviroportal.sk was introduced into service in testing operation in April 2005. Perspectives of Enviroportal.sk are discussed

  11. S.K. De Datta receives Clifton Garvin Fellowship

    OpenAIRE

    Felker, Susan B.

    2004-01-01

    S.K. De Datta, of Blacksburg, associate provost for international affairs at Virginia Tech, received the university's Clifton Garvin Fellowship Award. The award was conferred by the Virginia Tech Board of Visitors at its quarterly meeting Monday, Nov. 8.

  12. Infantile Onset Myasthenia Gravis with MuSK Antibodies

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap

    2006-08-01

    Full Text Available A late infantile case of myasthenia gravis (MG with anti-muscle-specific receptor tyrosine kinase (MuSK high antibody (Ab titer is reported from Kyushu University, Fukuoka; and Nagasaki University, Japan.

  13. Kun tabere kigger på skærmen

    DEFF Research Database (Denmark)

    Meier Sørensen, Bent

    2017-01-01

    Enhver, der har prøvet at tage en telefon fra et barn, ved, hvordan det må have været for Frodo at forsøge at fravriste Gollum ringen i " Ringenes Herre". Smartphones og andre skærme er blevet børns og alle andres " Precious", som Gollum kalder ringen. Vi rører mere ved skærme, end vi rører ved...

  14. Contribution of Somatic and Dendritic SK Channels in the Firing Rate of Deep Cerebellar Nuclei: Implication in Cerebellar Ataxia

    Directory of Open Access Journals (Sweden)

    Samira Abbasi

    2016-01-01

    Discussion: Therefore, inhibition of SK channel in DCN can cause cerebellar ataxia, and SK channel openers can have a therapeutic effect on cerebellar ataxia. In addition, the location of SK channels could be important in therapeutic goals. Dendritic SK channels can be a more effective target compared to somatic SK channels

  15. Uncovering a Role for SK2 in Angelman Syndrome

    Directory of Open Access Journals (Sweden)

    Sofia B. Lizarraga

    2015-07-01

    Full Text Available Angelman syndrome is a severe neurodevelopmental disorder caused by mutations in UBE3A. Sun et al. (2015 report SK2 as a UBE3A substrate and provide insight into the molecular mechanisms that might underlie impaired neuronal function in individuals affected by Angelman syndrome.

  16. CyPPA, a Positive SK3/SK2 Modulator, Reduces Activity of Dopaminergic Neurons, Inhibits Dopamine Release, and Counteracts Hyperdopaminergic Behaviors Induced by Methylphenidate

    DEFF Research Database (Denmark)

    Herrik, Kjartan F; Redrobe, John P; Holst, Dorte

    2012-01-01

    Dopamine (DA) containing midbrain neurons play critical roles in several psychiatric and neurological diseases, including schizophrenia and attention deficit hyperactivity disorder, and the substantia nigra pars compacta neurons selectively degenerate in Parkinson's disease. Pharmacological......]-amine (CyPPA), a subtype-selective positive modulator of SK channels (SK3¿>¿SK2¿>¿>¿>¿SK1, IK), decreased spontaneous firing rate, increased the duration of the apamin-sensitive afterhyperpolarization, and caused an activity-dependent inhibition of current-evoked action potentials in DA neurons from both...

  17. Global features of the Alcanivorax borkumensis SK2 genome

    DEFF Research Database (Denmark)

    Reva, Oleg N.; Hallin, Peter Fischer; Willenbrock, Hanni

    2008-01-01

    The global feature of the completely sequenced Alcanivorax borkumensis SK2 type strain chromosome is its symmetry and homogeneity. The origin and terminus of replication are located opposite to each other in the chromosome and are discerned with high signal to noise ratios by maximal oligonucleot......The global feature of the completely sequenced Alcanivorax borkumensis SK2 type strain chromosome is its symmetry and homogeneity. The origin and terminus of replication are located opposite to each other in the chromosome and are discerned with high signal to noise ratios by maximal...... oligonucleotide usage biases on the leading and lagging strand. Genomic DNA structure is rather uniform throughout the chromosome with respect to intrinsic curvature, position preference or base stacking energy. The orthologs and paralogs of A. borkumensis genes with the highest sequence homology were found...

  18. Pääsküla raamatukogu / Maris Takk

    Index Scriptorium Estoniae

    Takk, Maris

    2006-01-01

    Pääsküla raamatukogu (Tallinn, Pärnu mnt. 480A) sisekujundus, mille eest sisekujundajad Katrin Kaevats ja Peeter Pere (AB Muru & Pere) pälvisid Eesti Sisearhitektide Liidu 2005. a. ühiskondliku interjööri preemia. Ehitaja: OÜ SCE Grupp. K. Kaevatsist, tema tähtsamad tööd. P. Perest, valik AB Muru & Pere projektidest. Ill.: kaks korruste plaani, 17 värv. vaadet, K. Kaevatsi ja P. Pere fotod

  19. Pozytywizm, racjonalizm i... romantyzm Marii Skłodowskiej-Curie

    Directory of Open Access Journals (Sweden)

    Barbara Petelenz

    2015-12-01

    Full Text Available The International Year of Chemistry (2011, intertwined with commemoration of the Nobel Prize in Chemistry awarded in 1911 to Marie Skłodowska-Curie, made me to ask about the philosophical background of this outstanding woman. The first factor which I could see was the positivism, launched by August Comte in France and developed a few decades later by his Polish followers. Another factor which seemed to me important was the interplay between the emotional (romantic and intellectual (positivistic attitudes among the Poles in the 19th century. In her research, Marie Skłodowska-Curie used the positivistic, rationalistic method. This has led her (jointly with her husband Pierre Curie to elucidation of the radiation phenomena discovered by Henri Becquerel in 1896. The research initiated by the Curies (rewarded by the half of the Nobel Prize in Physics 1903 caused a subversion of the 19th century’s views on the structure of Matter. The way to such spectacular results must have been paved not only by the positivistic intellectual discipline but also by a dose of romantic enchantment. In the applicative terms, Marie Skłodowska-Curie became a pioneer of the evidence-based medicine. In moral terms, she represented a rare example of the practical altruism, inspired indirectly by Christianity, and directly by the Comte’s “religion of Humanity”.

  20. Selective activation of heteromeric SK channels contributes to action potential repolarization in mouse atrial myocytes.

    Science.gov (United States)

    Hancock, Jane M; Weatherall, Kate L; Choisy, Stéphanie C; James, Andrew F; Hancox, Jules C; Marrion, Neil V

    2015-05-01

    Activation of small conductance calcium-activated potassium (SK) channels is proposed to contribute to repolarization of the action potential in atrial myocytes. This role is controversial, as these cardiac SK channels appear to exhibit an uncharacteristic pharmacology. The objectives of this study were to resolve whether activation of SK channels contributes to atrial action potential repolarization and to determine the likely subunit composition of the channel. The effect of 2 SK channel inhibitors was assessed on outward current evoked in voltage clamp and on action potential duration in perforated patch and whole-cell current clamp recording from acutely isolated mouse atrial myocytes. The presence of SK channel subunits was assessed using immunocytochemistry. A significant component of outward current was reduced by the SK channel blockers apamin and UCL1684. Block by apamin displayed a sensitivity indicating that this current was carried by homomeric SK2 channels. Action potential duration was significantly prolonged by UCL1684, but not by apamin. This effect was accompanied by an increase in beat-to-beat variability and action potential triangulation. This pharmacology was matched by that of expressed heteromeric SK2-SK3 channels in HEK293 cells. Immunocytochemistry showed that atrial myocytes express both SK2 and SK3 channels with an overlapping expression pattern. Only proposed heteromeric SK2-SK3 channels are physiologically activated to contribute to action potential repolarization, which is indicated by the difference in pharmacology of evoked outward current and prolongation of atrial action potential duration. The effect of blocking this channel on the action potential suggests that SK channel inhibition during cardiac function has the potential to be proarrhythmic. Copyright © 2015 Heart Rhythm Society. Published by Elsevier Inc. All rights reserved.

  1. Coupling of SK channels, L-type Ca2+ channels, and ryanodine receptors in cardiomyocytes.

    Science.gov (United States)

    Zhang, Xiao-Dong; Coulibaly, Zana A; Chen, Wei Chun; Ledford, Hannah A; Lee, Jeong Han; Sirish, Padmini; Dai, Gu; Jian, Zhong; Chuang, Frank; Brust-Mascher, Ingrid; Yamoah, Ebenezer N; Chen-Izu, Ye; Izu, Leighton T; Chiamvimonvat, Nipavan

    2018-03-16

    Small-conductance Ca 2+ -activated K + (SK) channels regulate the excitability of cardiomyocytes by integrating intracellular Ca 2+ and membrane potentials on a beat-to-beat basis. The inextricable interplay between activation of SK channels and Ca 2+ dynamics suggests the pathology of one begets another. Yet, the exact mechanistic underpinning for the activation of cardiac SK channels remains unaddressed. Here, we investigated the intracellular Ca 2+ microdomains necessary for SK channel activation. SK currents coupled with Ca 2+ influx via L-type Ca 2+ channels (LTCCs) continued to be elicited after application of caffeine, ryanodine or thapsigargin to deplete SR Ca 2+ store, suggesting that LTCCs provide the immediate Ca 2+ microdomain for the activation of SK channels in cardiomyocytes. Super-resolution imaging of SK2, Ca v 1.2 Ca 2+ channel, and ryanodine receptor 2 (RyR2) was performed to quantify the nearest neighbor distances (NND) and localized the three molecules within hundreds of nanometers. The distribution of NND between SK2 and RyR2 as well as SK2 and Ca v 1.2 was bimodal, suggesting a spatial relationship between the channels. The activation mechanism revealed by our study paved the way for the understanding of the roles of SK channels on the feedback mechanism to regulate the activities of LTCCs and RyR2 to influence local and global Ca 2+ signaling.

  2. Hepatoma SK Hep-1 cells exhibit characteristics of oncogenic mesenchymal stem cells with highly metastatic capacity.

    Directory of Open Access Journals (Sweden)

    Jong Ryeol Eun

    Full Text Available SK Hep-1 cells (SK cells derived from a patient with liver adenocarcinoma have been considered a human hepatoma cell line with mesenchymal origin characteristics, however, SK cells do not express liver genes and exhibit liver function, thus, we hypothesized whether mesenchymal cells might contribute to human liver primary cancers. Here, we characterized SK cells and its tumourigenicity.We found that classical mesenchymal stem cell (MSC markers were presented on SK cells, but endothelial marker CD31, hematopoietic markers CD34 and CD45 were negative. SK cells are capable of differentiate into adipocytes and osteoblasts as adipose-derived MSC (Ad-MSC and bone marrow-derived MSC (BM-MSC do. Importantly, a single SK cell exhibited a substantial tumourigenicity and metastatic capacity in immunodefficient mice. Metastasis not only occurred in circulating organs such as lung, liver, and kidneys, but also in muscle, outer abdomen, and skin. SK cells presented greater in vitro invasive capacity than those of Ad-MSC and BM-MSC. The xenograft cells from subcutaneous and metastatic tumors exhibited a similar tumourigenicity and metastatic capacity, and showed the same relatively homogenous population with MSC characteristics when compared to parental SK cells. SK cells could unlimitedly expand in vitro without losing MSC characteristics, its tumuorigenicity and metastatic capacity, indicating that SK cells are oncogenic MSC with enhanced self-renewal capacity. We believe that this is the first report that human MSC appear to be transformed into cancer stem cells (CSC, and that their derivatives also function as CSCs.Our findings demonstrate that SK cells represent a transformation mechanism of normal MSC into an enhanced self-renewal CSC with metastasis capacity, SK cells and their xenografts represent a same relative homogeneity of CSC with substantial metastatic capacity. Thus, it represents a novel mechanism of tumor initiation, development and

  3. K. Sridhar Moorthy's Theoretical Modelling in Marketing - A Review

    African Journals Online (AJOL)

    Toshiba

    experimental design for theoretical modelling of sales force compensation is vivid and ... different from the concept of a model in decision support systems and behavioural .... ―refers to the fact that people may not optimize.‖ This, of course, is.

  4. Constant envelope OFDM scheme for 6PolSK-QPSK

    Science.gov (United States)

    Li, Yupeng; Ding, Ding

    2018-03-01

    A constant envelope OFDM scheme with phase modulator (PM-CE-OFDM) for 6PolSK-QPSK modulation was demonstrated. Performance under large fiber launch power is measured to check its advantages in counteracting fiber nonlinear impairments. In our simulation, PM-CE-OFDM, RF-assisted constant envelope OFDM (RF-CE-OFDM) and conventional OFDM (Con-OFDM) are transmitted through 80 km standard single mode fiber (SSMF) single channel and WDM system. Simulation results confirm that PM-CE-OFDM has best performance in resisting fiber nonlinearity. In addition, benefiting from the simple system structure, the complexity and cost of PM-CE-OFDM system could be reduced effectively.

  5. Subtype-specific, bi-component inhibition of SK channels by low internal pH

    DEFF Research Database (Denmark)

    Peitersen, Torben; Jespersen, Thomas; Jorgensen, Nanna K

    2006-01-01

    The effects of low intracellular pH (pH(i) 6.4) on cloned small-conductance Ca2+-activated K+ channel currents of all three subtypes (SK1, SK2, and SK3) were investigated in HEK293 cells using the patch-clamp technique. In 400 nM internal Ca2+ [Ca2+]i, all subtypes were inhibited by pH(i) 6...

  6. Dispersant Application during SG Wet Layup at SK Unit 1

    Energy Technology Data Exchange (ETDEWEB)

    Kwon, Hyukchul; Lee, Dooho; Sung, Kibang [KHNP Central Institute, Daejeon (Korea, Republic of)

    2014-05-15

    The corrosion products in the feedwater are deposited onto the steam generators (SGs) despite the effort to control them within limit of impurity. This deposit is one of causes for occurrence of SCC (Stress Corrosion Cracking), water level fluctuation and further corrosion of SGs. To minimize corrosion and remove deposit, the nuclear power plants apply high pH to the secondary system and SG chemical cleaning, respectively. But these methods can be costly and carry risks of extended outages or incomplete cleaning. Another method is an on-line dispersant application. The role of dispersant is to make deposit suspended in the SGs. Then, the suspended deposit is discharged to the blowdown system. The iron removal is increased in the blowdown system during the dispersant application. Additional significant benefit in the form of reduced corrosion product transport may be obtained through applying dispersant in the SGs wet lay operational mode. This method helps to reduce the total SGs loading without affecting critical outage activities and with minimal additional effort on the part of the utilities. This study provides the results of the dispersant application trial during the SG wet layup at SK Unit 1. As the PAA concentrations were increased, the corrosion rates of Alloy 690 and SA 106 Gr.B were increased. The corrosion rate of Alloy 690 was 2 times less than that of SA 106 Gr.B at 100 ppm of PAA based on the electrochemical experimental. There were no significant feasibility problems with application of PAA during the SG wet layup. The reasonable estimation of the additional mass removed by the presence of PAA during SGs wet layup is 460 g. The iron removal depended on PAA concentration injected based on the comparative results of the SK Unit 1 and TMI-1. It is expected that injection of PAA into the SG result in a significant decrease in the amount of iron transported to the SGs during the startup.

  7. Influence of source batch Sk dispersion on dosimetry for prostate cancer treatment with permanent implants

    DEFF Research Database (Denmark)

    Nunez-Cumplido, Eduardo; Perez-Calatayud, J; Casares-Magaz, O

    2015-01-01

    PURPOSE: In clinical practice, specific air kerma strength (SK) value is used in treatment planning system (TPS) permanent brachytherapy implant calculations with (125)I and (103)Pd sources; in fact, commercial TPS provide only one SK input value for all implanted sources and the certified shipment...

  8. Pharmacokinetics of the vasopressin antagonist, SK and F 101926, a synthetic octapeptide, in the rat

    International Nuclear Information System (INIS)

    Lynn, R.K.; Straub, K.M.; Landvatter, S.W.; Garvie, C.T.

    1986-01-01

    SK and F 101926 is a synthetic analogue of vasopressin which antagonizes vasopressin induced antidiuresis. Radiolabeled SK and F 101926 ( 3 H-3,3-D-TYR) was administered i.v. as a single bolus to male Sprague Dawley rats. Serial plasma samples were analyzed for SK and F 101926 by HPLC/LSC. The plasma concentration time curve for SK and F 101926 displayed three disposition phases. However, 80% of the total area under the curve was represented in one phase which displayed a half-life (t 1/2) of 20 min. The total systematic clearance (Cl) and the steady state volume of distribution (Vdss) were 15 ml/min/kg and 450 ml/kg, respectively. The t 1/2, Cl and Vdss were identical following doses of 10 and 100 μg/kg. 3 H-SK and F 101926 accounted for > 80% of the plasma radiolabel 0-30 min after dosing. However, by 12 hr, 3 H-SK and F 101926 represented only 6% of the plasma radiolabel. Following oral administration of 3 H-SK and F 101926, < 5% of the administered radiolabel reached the systematic circulation; however, no parent compound was detectable in plasma. These studies demonstrate that although SK and F 101926 has a relatively low systematic clearance, it also displays a relatively small volume of distribution which together result in a moderate beta elimination half-life

  9. Mitochondria-targeted antioxidant SkQ1 improves impaired dermal wound healing in old mice.

    Science.gov (United States)

    Demyanenko, Ilya A; Popova, Ekaterina N; Zakharova, Vlada V; Ilyinskaya, Olga P; Vasilieva, Tamara V; Romashchenko, Valeria P; Fedorov, Artem V; Manskikh, Vasily N; Skulachev, Maxim V; Zinovkin, Roman A; Pletjushkina, Olga Yu; Skulachev, Vladimir P; Chernyak, Boris V

    2015-07-01

    The process of skin wound healing is delayed or impaired in aging animals. To investigate the possible role of mitochondrial reactive oxygen species (mtROS) in cutaneous wound healing of aged mice, we have applied the mitochondria-targeted antioxidant SkQ1. The SkQ1 treatment resulted in accelerated resolution of the inflammatory phase, formation of granulation tissue, vascularization and epithelization of the wounds. The wounds of SkQ1-treated mice contained increased amount of myofibroblasts which produce extracellular matrix proteins and growth factors mediating granulation tissue formation. This effect resembled SkQ1-induced differentiation of fibroblasts to myofibroblast, observed earlierin vitro. The Transforming Growth Factor beta (TGFb) produced by SkQ1-treated fibroblasts was found to stimulated motility of endothelial cells in vitro, an effect which may underlie pro-angiogenic action of SkQ1 in the wounds. In vitro experiments showed that SkQ1 prevented decomposition of VE-cadherin containing contacts and following increase in permeability of endothelial cells monolayer, induced by pro-inflammatory cytokine TNF. Prevention of excessive reaction of endothelium to the pro-inflammatory cytokine(s) might account for anti-inflammatory effect of SkQ1. Our findings point to an important role of mtROS in pathogenesis of age-related chronic wounds.

  10. SK2 channels regulate mitochondrial respiration and mitochondrial Ca2+ uptake

    NARCIS (Netherlands)

    Honrath, Birgit; Matschke, Lina; Meyer, Tammo; Magerhans, Lena; Perocchi, Fabiana; Ganjam, Goutham K; Zischka, Hans; Krasel, Cornelius; Gerding, Albert; Bakker, Barbara M; Bünemann, Moritz; Strack, Stefan; Decher, Niels; Culmsee, Carsten; Dolga, Amalia M

    Mitochondrial calcium ([Ca(2+)]m) overload and changes in mitochondrial metabolism are key players in neuronal death. Small conductance calcium-activated potassium (SK) channels provide protection in different paradigms of neuronal cell death. Recently, SK channels were identified at the inner

  11. Complete sequences of four plasmids of Lactococcus lactis subsp cremoris SK11 reveal extensive adaptation to the dairy environment

    NARCIS (Netherlands)

    Siezen, R.J.; Renckens, B.; Swam, van I.; Peters, S.; Kranenburg, van R.; Kleerebezem, M.; Vos, de W.M.

    2005-01-01

    Lactococcus lactis strains are known to carry plasmids encoding industrially important traits. L. lactis subsp. cremoris SK11 is widely used by the dairy industry in cheese making. Its complete plasmid complement was sequenced and found to contain the plasmids pSK11A (10,372 bp), pSK11B (13,332 bp),

  12. SK2 channels regulate mitochondrial respiration and mitochondrial Ca2+ uptake.

    Science.gov (United States)

    Honrath, Birgit; Matschke, Lina; Meyer, Tammo; Magerhans, Lena; Perocchi, Fabiana; Ganjam, Goutham K; Zischka, Hans; Krasel, Cornelius; Gerding, Albert; Bakker, Barbara M; Bünemann, Moritz; Strack, Stefan; Decher, Niels; Culmsee, Carsten; Dolga, Amalia M

    2017-05-01

    Mitochondrial calcium ([Ca 2+ ] m ) overload and changes in mitochondrial metabolism are key players in neuronal death. Small conductance calcium-activated potassium (SK) channels provide protection in different paradigms of neuronal cell death. Recently, SK channels were identified at the inner mitochondrial membrane, however, their particular role in the observed neuroprotection remains unclear. Here, we show a potential neuroprotective mechanism that involves attenuation of [Ca 2+ ] m uptake upon SK channel activation as detected by time lapse mitochondrial Ca 2+ measurements with the Ca 2+ -binding mitochondria-targeted aequorin and FRET-based [Ca 2+ ] m probes. High-resolution respirometry revealed a reduction in mitochondrial respiration and complex I activity upon pharmacological activation and overexpression of mitochondrial SK2 channels resulting in reduced mitochondrial ROS formation. Overexpression of mitochondria-targeted SK2 channels enhanced mitochondrial resilience against neuronal death, and this effect was inhibited by overexpression of a mitochondria-targeted dominant-negative SK2 channel. These findings suggest that SK channels provide neuroprotection by reducing [Ca 2+ ] m uptake and mitochondrial respiration in conditions, where sustained mitochondrial damage determines progressive neuronal death.

  13. Pharmacokinetic/Toxicity Properties of the New Anti-Staphylococcal Lead Compound SK-03-92

    Directory of Open Access Journals (Sweden)

    William R. Schwan

    2015-11-01

    Full Text Available Because of the potential of a new anti-staphylococcal lead compound SK-03-92 as a topical antibiotic, a patch, or an orally active drug, we sought to determine its safety profile and oral bioavailability. SK-03-92 had a high IC50 (125 μg/mL in vitro against several mammalian cell lines, and mice injected intraperiteonally at the highest dose did not exhibit gross toxicity (e.g., altered gait, ungroomed, significant weight loss. Single dose (100 μg/g pharmacokinetic (PK analysis with formulated SK-03-92 showed that peak plasma concentration (1.64 μg/mL was achieved at 20–30 min. Oral relative bioavailability was 8%, and the drug half-life was 20–30 min, demonstrating that SK-03-92 is likely not a candidate for oral delivery. Five-day and two-week PK analyses demonstrated that SK-03-92 plasma levels were low. Multi-dose analysis showed no gross adverse effects to the mice and a SK-03-92 peak plasma concentration of 2.12 μg/mL with the presence of significant concentrations of breakdown products 15 min after dosing. SK-03-92 appeared to be very safe based on tissue culture and mouse gross toxicity determinations, but the peak plasma concentration suggests that a pro-drug of SK-03-92 or preparation of analogs of SK-03-92 with greater bioavailability and longer half-lives are warranted.

  14. The SK3 channel promotes placental vascularization by enhancing secretion of angiogenic factors.

    Science.gov (United States)

    Rada, Cara C; Murray, Grace; England, Sarah K

    2014-11-15

    Proper placental perfusion is essential for fetal exchange of oxygen, nutrients, and waste with the maternal circulation. Impairment of uteroplacental vascular function can lead to pregnancy complications, including preeclampsia and intrauterine growth restriction (IUGR). Potassium channels have been recognized as regulators of vascular proliferation, angiogenesis, and secretion of vasoactive factors, and their dysfunction may underlie pregnancy-related vascular diseases. Overexpression of one channel in particular, the small-conductance calcium-activated potassium channel 3 (SK3), is known to increase vascularization in mice, and mice overexpressing the SK3 channel (SK3(T/T) mice) have a high rate of fetal demise and IUGR. Here, we show that overexpression of SK3 causes fetal loss through abnormal placental vascularization. We previously reported that, at pregnancy day 14, placentas isolated from SK3(T/T) mice are smaller than those obtained from wild-type mice. In this study, histological analysis reveals that SK3(T/-) placentas at this stage have abnormal placental morphology, and microcomputed tomography shows that these placentas have significantly larger and more blood vessels than those from wild-type mice. To identify the mechanism by which these vascularization defects occur, we measured levels of vascular endothelial growth factor (VEGF), placental growth factor, and the soluble form of VEGF receptor 1 (sFlt-1), which must be tightly regulated to ensure proper placental development. Our data reveal that overexpression of SK3 alters systemic and placental ratios of the angiogenic factor VEGF to antiangiogenic factor sFlt-1 throughout pregnancy. Additionally, we observe increased expression of hypoxia-inducing factor 2α in SK3(T/-) placentas. We conclude that the SK3 channel modulates placental vascular development and fetal health by altering VEGF signaling. Copyright © 2014 the American Physiological Society.

  15. Development of norepinephrine transporter reuptake inhibition assays using SK-N-BE(2C cells

    Directory of Open Access Journals (Sweden)

    Ann M. Decker

    2018-05-01

    Full Text Available This report describes efforts to develop and validate novel norepinephrine transporter reuptake inhibition assays using human neuroblastoma SK-N-BE(2C cells in 24-well format. Before conducting the assays, the SK-N-BE(2C cells were first evaluated for their ability to uptake [3H]norepinephrine and were shown to have a saturable uptake with a KM value of 416 nM. Using this determined KM value, reuptake inhibition assays were then conducted with a variety of ligands including antidepressants, as well as piperazine and phenyltropane derivatives. The results obtained with the SK-N-BE(2C cells indicate that this model system can detect a range of ligand potencies, which compare well with other established transporter assays. Our data suggest that SK-N-BE(2C cells have potential utility to serve as another model system to detect norepinephrine reuptake inhibition activity.

  16. Use of solar parabolic cookers (SK-14) in Nepal

    Energy Technology Data Exchange (ETDEWEB)

    Shrestha, S. [Asia Network for Small Scale Bioresources, Kathmandu (Nepal)

    2000-07-01

    Solar Cooker is a device that uses only sunlight to cook food and pasteurise water. Solar cooker can be used along with other cooking devices to save cost, fuel and the time spent in gathering fuelwood. Solar cooking enables individual families to do without commercially sold fuel and help save money. In Nepal, supply of energy is one of the major problems for both urban and rural households. Increase in population, high migration, expensive fuel bills, environmental degradation, and unsafe drinking water have resulted in the keen interest from people of Nepal in the use of solar energy. The increasing number of tourists and trekkers are now one of the major sources of income and many people are engaged in running hotels, lodges, and restaurants. This has also increased the fuel demand. This paper highlights the current energy situation of Nepal, the technical details of solar parabolic cooker (SK-14), its uses throughout Nepal, strategies adopted by various organisations for its promotion. A lot of effort have been made by various organisations, educational, governmental and health related institutions in order to introduce solar cooking programs in villages of Nepal. The parameters, which have influenced the adoption of this technology in Nepal are also mentioned. Various awareness programs and the government subsidy program are playing considerable role in dissemination of such technologies. The promotion activities with the objective of mass awareness have long term effect and sustainable rather than instant business. Continued efforts to create awareness, development of models as well as proper promotion and dissemination are required. (au)

  17. Characterization of CD4 and CD8 T Cell Responses in MuSK Myasthenia Gravis

    Science.gov (United States)

    Yi, JS; Guidon, A; Sparks, S; Osborne, R; Juel, VC; Massey, JM; Sanders, DB; Weinhold, KJ; Guptill, JT

    2014-01-01

    Muscle specific tyrosine kinase myasthenia gravis (MuSK MG) is a form of autoimmune MG that predominantly affects women and has unique clinical features, including prominent bulbar weakness, muscle atrophy, and excellent response to therapeutic plasma exchange. Patients with MuSK MG have predominantly IgG4 autoantibodies directed against MuSK on the postsynaptic muscle membrane. Lymphocyte functionality has not been reported in this condition. The goal of this study was to characterize T-cell responses in patients with MuSK MG. Intracellular production of IFN-gamma, TNF-alpha, IL-2, IL-17, and IL-21 by CD4+ and CD8+ T-cells was measured by polychromatic flow cytometry in peripheral blood samples from 11 Musk MG patients and 10 healthy controls. Only one MuSK MG patient was not receiving immunosuppressive therapy. Regulatory T-cells (Treg) were also included in our analysis to determine if changes in T cell function were due to altered Treg frequencies. CD8+ T-cells from MuSK MG patients had higher frequencies of polyfunctional responses than controls, and CD4+ T-cells had higher IL-2, TNF-alpha, and IL-17. MuSK MG patients had a higher percentage of CD4+ T-cells producing combinations of IFN-gamma/IL-2/TNF-gamma, TNF-alpha/IL-2, and IFN-gamma/TNF-alpha. Interestingly, Treg numbers and CD39 expression were not different from control values. MuSK MG patients had increased frequencies of Th1 and Th17 cytokines and were primed for polyfunctional proinflammatory responses that cannot be explained by a defect in Treg function or number. PMID:24378287

  18. Distribution, expression and functional effects of small conductance Ca-activated potassium (SK) channels in rat myometrium.

    Science.gov (United States)

    Noble, Karen; Floyd, Rachel; Shmygol, Andre; Shmygol, Anatoly; Mobasheri, A; Wray, Susan

    2010-01-01

    Calcium-activated potassium channels are important in a variety of smooth muscles, contributing to excitability and contractility. In the myometrium previous work has focussed on the large conductance channels (BK), and the role of small conductance channels (SK) has received scant attention, despite the finding that over-expression of an SK channel isoform (SK3) results in uterine dysfunction and delayed parturition. This study therefore characterises the expression of the three SK channel isoforms (SK1-3) in rat myometrium throughout pregnancy and investigates their effect on cytosolic [Ca] and force and compares this with that of BK channels. Consistent expression of all SK isoform transcripts and clear immunostaining of SK1-3 was found. Inhibition of SK1-3 channels (apamin, scyllatoxin) significantly inhibited outward current, caused membrane depolarisation and elicited action potentials in previously quiescent cells. Apamin or scyllatoxin increased the amplitude of [Ca] and force in spontaneously contracting myometrial strips throughout gestation. The functional effect of SK inhibition was larger than that of BK channel inhibition. Thus we show for the first time that SK1-3 channels are expressed and translated throughout pregnancy and contribute to outward current, regulate membrane potential and hence Ca signals in pregnant rat myometrium. They contribute more to quiescence that BK channels. 2009 Elsevier Ltd. All rights reserved.

  19. Pomegranate inhibits neuroinflammation and amyloidogenesis in IL-1β-stimulated SK-N-SH cells.

    Science.gov (United States)

    Velagapudi, Ravikanth; Baco, Gina; Khela, Sunjeet; Okorji, Uchechukwu; Olajide, Olumayokun

    2016-06-01

    Pomegranate fruit, Punica granatum L. (Punicaceae), and its constituents have been shown to inhibit inflammation. In this study, we aimed to assess the effects of freeze-dried pomegranate (PWE) on PGE2 production in IL-1β-stimulated SK-N-SH cells. An enzyme immunoassay (EIA) was used to measure prostaglandin E2 (PGE2) production from supernatants of IL-1β-stimulated SK-N-SH cells. Expression of COX-2, phospho-IκB, and phospho-IKK proteins was evaluated, while NF-κB reporter gene assay was carried out in TNFα-stimulated HEK293 cells to determine the effect of PWE on NF-κB transactivation. Levels of BACE-1 and Aβ in SK-N-SH cells stimulated with IL-1β were measured with an in cell ELISA. PWE (25-200 μg/ml) dose dependently reduced COX-2-dependent PGE2 production in SK-N-SH cells stimulated with IL-1β. Phosphorylation of IκB and IKK was significantly (p pomegranate inhibits inflammation, as well as amyloidogenesis in IL-1β-stimulated SK-N-SH cells. We propose that pomegranate is a potential nutritional strategy in slowing the progression of neurodegenerative disorders such as Alzheimer's disease.

  20. The solar kettle-thermos flask (SK-TF) and solar vacuum tube oven

    Energy Technology Data Exchange (ETDEWEB)

    Yak, Alex Kee Koo [AkayConsult Enterprise, Johor Bahru (Malaysia)

    2008-07-01

    The Solar Kettle-Thermos Flask (SK-TF) and Solar Vacuum Tube Oven (SaVeTao): A Cost Effective, Sustainable and Renewable Water Pasteurization and Food Processing System For The Developing World. Based on the perfect solar thermal energy harvesting paradigm of maximum solar radiation absorption and minimum loss of stored converted solar thermal energy, Solar Vacuum Glass Tubes (SVGT) indefinitely delivers solar pasteurized safe drinking water, powered solely by free solar energy. The SVGT is the heart of the SK-TF. Being vacuum insulated, the SK-TF doubles up as a vacuum flask, delivering stored solar heated water in the morning before the Sun is up. With a high stagnation temperature of more than 200 C, the SK-TF can also be used for other heating purposes e.g. an oven or autoclave. Powered solely by free solar energy, the SK-TF and SaVeTaO could very well be the answer in providing safe solar pasteurized drinking water and cooking to the global poor and needy in a sustainable and renewable way. (orig.)

  1. NS309 decreases rat detrusor smooth muscle membrane potential and phasic contractions by activating SK3 channels

    Science.gov (United States)

    Parajuli, Shankar P; Hristov, Kiril L; Soder, Rupal P; Kellett, Whitney F; Petkov, Georgi V

    2013-01-01

    Background and Purpose Overactive bladder (OAB) is often associated with abnormally increased detrusor smooth muscle (DSM) contractions. We used NS309, a selective and potent opener of the small or intermediate conductance Ca2+-activated K+ (SK or IK, respectively) channels, to evaluate how SK/IK channel activation modulates DSM function. Experimental Approach We employed single-cell RT-PCR, immunocytochemistry, whole cell patch-clamp in freshly isolated rat DSM cells and isometric tension recordings of isolated DSM strips to explore how the pharmacological activation of SK/IK channels with NS309 modulates DSM function. Key Results We detected SK3 but not SK1, SK2 or IK channels expression at both mRNA and protein levels by RT-PCR and immunocytochemistry in DSM single cells. NS309 (10 μM) significantly increased the whole cell SK currents and hyperpolarized DSM cell resting membrane potential. The NS309 hyperpolarizing effect was blocked by apamin, a selective SK channel inhibitor. NS309 inhibited the spontaneous phasic contraction amplitude, force, frequency, duration and tone of isolated DSM strips in a concentration-dependent manner. The inhibitory effect of NS309 on spontaneous phasic contractions was blocked by apamin but not by TRAM-34, indicating no functional role of the IK channels in rat DSM. NS309 also significantly inhibited the pharmacologically and electrical field stimulation-induced DSM contractions. Conclusions and Implications Our data reveal that SK3 channel is the main SK/IK subtype in rat DSM. Pharmacological activation of SK3 channels with NS309 decreases rat DSM cell excitability and contractility, suggesting that SK3 channels might be potential therapeutic targets to control OAB associated with detrusor overactivity. PMID:23145946

  2. Small-conductance calcium-activated potassium (SK) channels contribute to action potential repolarization in human atria

    DEFF Research Database (Denmark)

    Skibsbye, Lasse; Poulet, Claire; Diness, Jonas Goldin

    2014-01-01

    (+) currents by ∼15% and prolonged action potential duration (APD), but no effect was observed in myocytes from AF patients. In trabeculae muscle strips from right atrial appendages of SR patients, both compounds increased APD and effective refractory period, and depolarized the resting membrane potential......, while only NS8593 induced these effects in tissue from AF patients. SK channel inhibition did not alter any electrophysiological parameter in human interventricular septum tissue. CONCLUSIONS: SK channels are present in human atria where they participate in repolarization. SK2 and SK3 were down...

  3. The spatial distribution of rock landforms in the Pohořská Mountains (Pohořská hornatina), Czech Republic

    Czech Academy of Sciences Publication Activity Database

    Rypl, J.; Kirchner, Karel; Blažek, M.

    2017-01-01

    Roč. 57, č. 2 (2017), s. 45-55 ISSN 1581-6613 Institutional support: RVO:68145535 Keywords : geomorphology * rock landforms * lithology * Pohořská Mountains Subject RIV: DE - Earth Magnetism, Geodesy, Geography OBOR OECD: Physical geography Impact factor: 0.533, year: 2016 http://www.pf.jcu.cz/structure/departments/kge/upload/files/1184-13377-1-PB.pdf

  4. Anti-MuSK-Positive Myasthenia Gravis in a Patient with Parkinsonism and Cognitive Impairment

    Directory of Open Access Journals (Sweden)

    S. Lanfranconi

    2011-01-01

    Full Text Available Muscle-specific tyrosine kinase- (MuSK- antibodies-positive Myasthenia Gravis accounts for about one third of Seronegative Myasthenia Gravis and is clinically characterized by early onset of prominent bulbar, neck, shoulder girdle, and respiratory weakness. The response to medical therapy is generally poor. Here we report a case of late-onset MuSK-antibodies-positive Myasthenia Gravis presenting with signs of cognitive impairment and parkinsonism in addition to bulbar involvement and external ophthalmoplegia. The pattern of involvement of both peripheral and central nervous system dysfunction might suggest a common pathogenic mechanism, involving impaired cholinergic transmission.

  5. The 5K70SK automatically tuned, high power, S-band klystron

    Science.gov (United States)

    Goldfinger, A.

    1977-01-01

    Primary objectives include delivery of 44 5K70SK klystron amplifier tubes and 26 remote tuner assemblies with spare parts kits. Results of a reliability demonstration on a klystron test cavity are discussed, along with reliability tests performed on a remote tuning unit. Production problems and one design modification are reported and discussed. Results of PAT and DVT are included.

  6. Protective roles for potassium SK/KCa2 channels in microglia and neurons

    Directory of Open Access Journals (Sweden)

    Amalia M Dolga

    2012-11-01

    Full Text Available New concepts on potassium channel function in neuroinflammation suggest that they regulate mechanisms of microglial activation, including intracellular calcium homeostasis, morphological alterations, pro-inflammatory cytokine release, antigen presentation, and phagocytosis. Although little is known about voltage independent potassium channels in microglia, special attention emerges on small (SK/KCNN1-3/KCa2 and intermediate (IK/KCNN4/KCa3.1-conductance calcium-activated potassium channels as regulators of microglial activation in the field of research on neuroinflammation and neurodegeneration. In particular, recent findings suggested that SK/KCa2 channels, by regulating calcium homeostasis, may elicit a dual mechanism of action with protective properties in neurons and inhibition of inflammatory responses in microglia. Thus, modulating SK/KCa2 channels and calcium signaling may provide novel therapeutic strategies in neurological disorders, where neuronal cell death and inflammatory responses concomitantly contribute to disease progression. Here, we review the particular role of SK/KCa2 channels for [Ca2+]i regulation in microglia and neurons, and we discuss the potential impact for further experimental approaches addressing novel therapeutic strategies in neurological diseases, where neuronal cell death and neuroinflammatory processes are prominent.

  7. Býčí skála Cave, Czech Republic: Radiocarbon dates of rock paintings

    Czech Academy of Sciences Publication Activity Database

    Svoboda, Jiří; van der Plicht, H.; Balák, I.

    2005-01-01

    Roč. 43, - (2005), s. 7-9 ISSN 1022-3282 Institutional research plan: CEZ:AV0Z80010507 Keywords : Býčí skála Czech Republic * rock art * radiocarbon dating Subject RIV: AC - Archeology, Anthropology, Ethnology

  8. Evaluating Cytotoxicity of Hyaluronate Targeted Solid Lipid Nanoparticles of Etoposide on SK-OV-3 Cells

    Directory of Open Access Journals (Sweden)

    Parviz Mohammadi Ghalaei

    2014-01-01

    Full Text Available The epithelial ovarian carcinoma is one of the most fatal gynecological cancers. Etoposide is used in treating platinum-resistant ovarian cancer. Sodium hyaluronate is a substance that binds to the CD44 receptors overexpressed in SK-OV-3 cells of epithelial ovarian carcinoma. The aim of the present work was to study the cytotoxicity effect of hyaluronate targeted solid lipid nanoparticles (SLNs of etoposide on SK-OV-3 cells. The cytotoxicity of the targeted and nontargeted SLNs of etoposide was compared to free drug on the SK-OV-3 cells by MTT assay method. The cellular uptake of the targeted and nontargeted nanoparticles containing sodium fluorescein was also studied. The difference of cell vitality between nontargeted nanoparticles and also targeted nanoparticles with free drug was significant. Targeted nanoparticles also caused more toxicity than nontargeted nanoparticles (P<0.05. After 4 hours of incubating, the fluorescence was remarkably higher in the cells treated by targeted SLNs rather than nontargeted ones, and there was no observable fluorescence in cells incubated with pure sodium fluorescein. Hyaluronate targeted SLNs containing etoposide increased the cytotoxicity of etoposide on SK-OV-3 cells which may be a worthwhile potential method for reducing the prescribed dose and systemic side effects of this drug in epithelial ovarian carcinoma.

  9. Hvem sidder dér bag skærmen?

    DEFF Research Database (Denmark)

    Werlauff, Erik; Schaumburg-Müller, Peer

    2009-01-01

    Med udgangspunkt i nutidens daytrader, der sidder ´"bag skærmen" og foretager talrige daglige køb og salg af børsnoterede aktier mv., analyseres det i artiklen, hvornår en sådan lovlig daytrading udarter til strafbar kursmanipulation. Nogle daytradere har ment, at hvis en ikke-professionel invest...

  10. Group SkSP-R sampling plan for accelerated life tests

    Indian Academy of Sciences (India)

    Muhammad Aslam

    2017-09-15

    Sep 15, 2017 ... SkSP-R sampling; life test; Weibull distribution; producer's risk; ... designed a sampling plan under a time-truncated life test .... adjusted using an acceleration factor. ... where P is the probability of lot acceptance for a single.

  11. [Roles of Y box-binding protein 1 in SK-BR-3 breast cancer proliferation].

    Science.gov (United States)

    Shi, Jianhong; Lü, Xinrui; Wang, Bing; Daudan, Lin; Yanan, Wang; Yuhui, Bu; Zhenfeng, Ma

    2014-09-30

    To explore the roles of Y box-binding protein 1 (YB-1) in breast cancer cell proliferation. Twenty cases of surgical removal of breast cancer tissue (diagnosed with invasive ductal carcinoma, stage II, by postoperative paraffin pathology) and normal breast tissues adjacent to carcinoma were collected during June 2013 to August 2013.Quantitative real-time PCR (qRT-PCR) was performed to detect the YB1 mRNA levels. Cultured mammary epithelial cells (HBL-100) and breast cancer cells (MCF7, MDA-MB-231 & SK-BR-3 cells) were harvested and qRT-PCR was performed to detect the YB1 mRNA levels.SK-BR-3 cells were stimulated with various concentrations of PDGF-BB and YB1 expression levels were detected by qRT-PCR. Down-regulation or over-expression of YB1 by si-YB1 or Ad-GFP-YB1 was detected in SK-BR-3 cells. And MTS cell proliferation assay kit was used to detect cell proliferation. YB1 mRNA levels were significantly higher in breast cancer tissues and MDA-MB-231 and SK-BR-3 breast cancer cell lines than that in adjacent normal breast tissues and HBL-100 mammary epithelial cells respectively (P BR-3 cells in a dose-dependent manner. A down-regulation of endogenous YB1 decreases and an over-expression of exogenous YB1 promotes the proliferation activity in SK-BR-3 cells.

  12. Gene expression in SK-Mel-28 human melanoma cells treated with the snake venom jararhagin.

    Science.gov (United States)

    Klein, Anelise; Capitanio, Juliana Silva; Maria, Durvanei Augusto; Ruiz, Itamar Romano Garcia

    2011-01-01

    Alternative approaches to improve the treatment of advanced melanomas are highly needed. The disintegrin domain of metalloproteinases binds integrin receptors on tumor cells, blocking migration, invasion, and metastatization. Previous studies showed that jararhagin, from the Bothrops jararaca snake venom, induces changes in the morphology and viability of SK-Mel-28 human melanoma cells, and decreases the number of metastases in mice injected with pre-treated cells. The purpose of this study was to evaluate the molecular effects of jararhagin on SK-Mel-28 cells and fibroblasts, concerning the expression of integrins, cadherins, caspases, and TP53 genes. Sub-toxic doses of jararhagin were administered to confluent cells. RT-PCR was performed following extraction of total RNA. Jararhagin treatments induced similar morphological alterations in both normal and tumor cells, with higher IC50 values for fibroblasts. Integrin genes were downregulated in untreated cells, except for ITGA6a,b, ITGAv, and ITGB3 which were highly expressed in SK-Mel-28. The integrin expression profiles were not affected by the toxin. However, jararhagin 30ng/μl upregulated genes TP53, CDKN1A, CDKN2A, CASP3, CASP5, CASP6, CASP8, and E-CDH in SK-Mel-28, and genes ITGB6, ITGB7, CASP3, TP53, and CDKN1B in fibroblasts. Appropriate jararhagin concentration can have apoptotic and suppressant effects on SK-Mel-28 cells, rather than on fibroblasts, and can be used to develop potential anti-cancer drugs. Copyright © 2010 Elsevier Ltd. All rights reserved.

  13. Optical spectroscopy of the blue supergiant Sk-69° 279 and its circumstellar shell with SALT

    Science.gov (United States)

    Gvaramadze, V. V.; Kniazev, A. Y.; Maryeva, O. V.; Berdnikov, L. N.

    2018-02-01

    We report the results of optical spectroscopy of the blue supergiant Sk-69° 279 and its circular shell in the Large Magellanic Cloud (LMC) with the Southern African Large Telescope (SALT). We classify Sk-69° 279 as an O9.2 Iaf star and analyse its spectrum by using the stellar atmosphere code CMFGEN, obtaining a stellar temperature of ≈30 kK, a luminosity of log (L*/ L⊙) = 5.54, a mass-loss rate of log (\\dot{M}/ M_{⊙} yr^{-1}) = -5.26, and a wind velocity of 800km s-1. We found also that Sk-69° 279 possesses an extended atmosphere with an effective temperature of ≈24 kK and that its surface helium and nitrogen abundances are enhanced, respectively, by factors of ≈2 and 20-30. This suggests that either Sk-69° 279 was initially a (single) fast-rotating ( ≳ 400 km s- 1) star, which only recently evolved off the main sequence, or that it is a product of close binary evolution. The long-slit spectroscopy of the shell around Sk-69° 279 revealed that its nitrogen abundance is enhanced by the same factor as the stellar atmosphere, which implies that the shell is composed mostly of the CNO processed material lost by the star. Our findings support previous propositions that some massive stars can produce compact circumstellar shells and, presumably, appear as luminous blue variables while they are still on the main sequence or have only recently left it.

  14. The 7SK snRNP associates with the little elongation complex to promote snRNA gene expression.

    Science.gov (United States)

    Egloff, Sylvain; Vitali, Patrice; Tellier, Michael; Raffel, Raoul; Murphy, Shona; Kiss, Tamás

    2017-04-03

    The 7SK small nuclear RNP (snRNP), composed of the 7SK small nuclear RNA (snRNA), MePCE, and Larp7, regulates the mRNA elongation capacity of RNA polymerase II (RNAPII) through controlling the nuclear activity of positive transcription elongation factor b (P-TEFb). Here, we demonstrate that the human 7SK snRNP also functions as a canonical transcription factor that, in collaboration with the little elongation complex (LEC) comprising ELL, Ice1, Ice2, and ZC3H8, promotes transcription of RNAPII-specific spliceosomal snRNA and small nucleolar RNA (snoRNA) genes. The 7SK snRNA specifically associates with a fraction of RNAPII hyperphosphorylated at Ser5 and Ser7, which is a hallmark of RNAPII engaged in snRNA synthesis. Chromatin immunoprecipitation (ChIP) and chromatin isolation by RNA purification (ChIRP) experiments revealed enrichments for all components of the 7SK snRNP on RNAPII-specific sn/snoRNA genes. Depletion of 7SK snRNA or Larp7 disrupts LEC integrity, inhibits RNAPII recruitment to RNAPII-specific sn/snoRNA genes, and reduces nascent snRNA and snoRNA synthesis. Thus, through controlling both mRNA elongation and sn/snoRNA synthesis, the 7SK snRNP is a key regulator of nuclear RNA production by RNAPII. © 2017 The Authors.

  15. [Roles of KLF5 in inhibition TNFα-induced SK-BR-3 breast cancer cell apoptosis].

    Science.gov (United States)

    Shi, Jianhong; Liu, Caiyun; Zhang, Anyi; Cui, Naipeng; Wang, Bing; Chen, Baoping; Ma, Zhenfeng

    2014-07-08

    To explore the expression levels and roles of Krüpple-like factor 5 (KLF5) in tumor necrosis factor α (TNFα)-induced SK-BR-3 breast cancer cells. SK-BR-3 breast cancer cells were stimulated by TNFα at different concentrations (0, 1, 5, 10, 20 µg/L) for specified durations (0, 6, 12, 24, 36 h). Western blot was performed to detect KLF5 protein levels. Then Western blot and quantitative real-time PCR (qRT-PCR) were used to detect the expression levels of apoptosis genes. Flow cytometry and qRT-PCR were used to observe the effects of exogenous KLF5 on TNFα-induced apoptosis of SK-BR-3 breast cancer cell. KLF5 expression levels significantly decreased in TNFα-stimulated SK-BR-3 breast cancer cells in a concentration- and time-dependent manner. Quantitative RT-PCR results showed that TNFα up-regulate apoptosis gene caspase 3, caspase 9 and bax expression levels and down-regulate bcl-1 level in SK-BR-3 cells. Adenovirus expression vectors of pAd-GFP and pAd-GFP-KLF5 were constructed and used to infect SK-BR-3 breast cancer cells. Over-expression of GFP-KLF5 inhibited apoptosis in TNFα-stimulated SK-BR-3 breast cancer cells. TNFα reduces KLF5 expression in SK-BR-3 breast cancer cells and KLF5 participates in TNFα-induced SK-BR-3 cell apoptosis.

  16. Observation of the decay B¯s0→ψ(2SK+π−

    Directory of Open Access Journals (Sweden)

    R. Aaij

    2015-07-01

    Full Text Available The decay B¯s0→ψ(2SK+π− is observed using a data set corresponding to an integrated luminosity of 3.0 fb−1 collected by the LHCb experiment in pp collisions at centre-of-mass energies of 7 and 8 TeV. The branching fraction relative to the B0→ψ(2SK+π− decay mode is measured to beB(B¯s0→ψ(2SK+π−B(B0→ψ(2SK+π−=5.38±0.36(stat±0.22(syst±0.31(fs/fd%, where fs/fd indicates the uncertainty due to the ratio of probabilities for a b quark to hadronise into a Bs0 or B0 meson. Using an amplitude analysis, the fraction of decays proceeding via an intermediate K⁎(8920 meson is measured to be 0.645±0.049(stat±0.049(syst and its longitudinal polarisation fraction is 0.524±0.056(stat±0.029(syst. The relative branching fraction for this component is determined to be B(B¯s0→ψ(2SK⁎(8920B(B0→ψ(2SK⁎(8920=5.58±0.57(stat±0.40(syst±0.32(fs/fd%. In addition, the mass splitting between the Bs0 and B0 mesons is measured as M(Bs0−M(B0=87.45±0.44(stat±0.09(syst MeV/c2.

  17. Production of prostate-specific antigen by a breast cancer cell line, Sk-Br-3

    International Nuclear Information System (INIS)

    Kamali Sarvestani, E.; Ghaderi, A.

    2002-01-01

    Prostate-specific antigen is a 33-KDa serine protease that is produced predominantly by prostate epithelium. However, it has been shown that about 30-40% of female breast tumors produce prostate-specific antigen and its production is associated with the presence of estrogen and progesterone receptors. We have now developed a new tissue culture system to study prostate-specific antigen production in breast cancer and its association with prognostic factors such as progesterone receptor and c-erbB-2. For this purpose we investigated the ability of prostate-specific antigen production in five different cell lines, including two breast cancer cell lines, Sk-Br-3 and MDA-MB-453. The prostate-specific antigen in tissue culture supernatant and cytoplasm of the Sk-Br-3 cell line was detected by western blotting and immunoperoxidase, respectively. Furthermore, we found lower expression of c-erbB-2 in Sk-Br-3 than non-prostate-specific antigen producer breast cancer cell line, MDA-MB-453. Progesterone receptor was expressed by both prostate-specific antigen-positive and -negative cell lines and only the intensity of staining and the number of positive cells in Sk-Br-3 population was higher than MDA-MB-453. According to our findings prostate-specific antigen can be considered as a good prognostic factor in breast cancer and we suggest that these two cell lines are a good in vitro model to study the relationship of different breast cancer prognostic factors and their regulations

  18. Polyploidization on SK-N-MC human neuroblastoma cells infected with herpes simplex virus 1.

    Science.gov (United States)

    Karalyan, Zaven; Izmailyan, Roza; Karalova, Elena; Abroyan, Liana; Hakobyan, Lina; Avetisyan, Aida; Semerjyan, Zara

    2016-01-01

    Polyploidization is one of the most dramatic changes occurring within cell genome owing to various reasons including under many viral infections. We examined the impact of herpes simplex virus-1 (HSV-1) on SK-N-MC human neuroblastoma cell line. The infected cells were followed from 6 hours up to 96 hours post infection (hpi). A large number of polyploid cells with giant nuclei was observed under the influence of HSV-1 at 24 hpi with the DNA content of 32c to 64c or more, in comparison with control SK-N-MC cells that were characterized by relatively moderate values of ploidy, i.e. 8с to 16с (where 1c is the haploid amount of nuclear DNA found in normal diploid populations in G0/G1). After 48-96 hpi, the population of polyploid cells with giant nuclei decreased to the benchmark level. The SK-NMC cells infected with HSV-1 for 24 hours were stained with gallocyanine and monitored for cytological features. The infected cells underwent virus induced cellcell and nuclei fusion with the formation of dense nuclei syncytium. The metabolic activity of HSV-1 infected cells was higher in both nuclei and nucleoli when compared to control cells.

  19. Dihydromyricetin induces cell cycle arrest and apoptosis in melanoma SK-MEL-28 cells.

    Science.gov (United States)

    Zeng, Guofang; Liu, Jie; Chen, Hege; Liu, Bin; Zhang, Qingyu; Li, Mingyi; Zhu, Runzhi

    2014-06-01

    Dihydromyricetin (DHM) exhibits multiple pharmacological activities; however, the role of DHM in anti-melanoma activities and the underlying molecular mechanisms are unclear. The aim of the present study was to evaluate the effects of DHM on cell proliferation, cell cycle distribution and apoptosis in the human melanoma SK-MEL-28 cell line, and to explore the related mechanisms. The effect of DHM on cell proliferation was investigated by MTT assay, and cell cycle distribution was determined by flow cytometry. TUNEL assay was used to evaluate DHM-mediated apoptosis, and western blotting was applied to examine expression levels of p53, p21, Cdc25A, Cdc2, P-Cdc2, Bax, IKK-α, NF-κB p65, p38 and P-p38 proteins. The results revealed that DHM suppressed cell proliferation of SK-MEL-28 cells in a concentration- and time-dependent manner, and caused cell cycle arrest at the G1/S phase. DHM increased the production of p53 and p21 proteins and downregulated the production of Cdc25A, Cdc2 and P-Cdc2 proteins, which induced cell cycle arrest. Additionally, DHM significantly induced the apoptosis of SK-MEL-28 cells, and enhanced the expression levels of Bax proteins and decreased the protein levels of IKK-α, NF-κB (p65) and P-p38. The results suggest that DHM may be a novel and effective candidate agent to inhibit the growth of melanoma.

  20. Technical Monitoring System for a New Part of Gdańsk Deepwater Container Terminal

    Directory of Open Access Journals (Sweden)

    Miśkiewicz Mikołaj

    2017-04-01

    Full Text Available Development strategy for the port of Gdańsk assumes that , up to 2027, it will become the main transport centre in the Baltic Sea region. To this end , for many years work has been carried out to facilitate access to the port both by sea and land. One of the elements of extension of the port is the building of another terminal of Deepwater Container Terminal, Gdańsk. In this task, geotechnical operations aimed at appropriate preparation of subgrade for storage of containers and operation of cranes, have been especially important. Effectiveness of the assumed design solution of land-side girder for gantry crane has been verified by means of a technical monitoring system. This paper presents main elements of design the land-side girder of a new quay of the container terminal in Gdańsk, together with its computational analysis, as well as the results collected during a few months of observation, which have revealed real work of monitored elements.

  1. hnRNP R and its main interactor, the noncoding RNA 7SK, coregulate the axonal transcriptome of motoneurons.

    Science.gov (United States)

    Briese, Michael; Saal-Bauernschubert, Lena; Ji, Changhe; Moradi, Mehri; Ghanawi, Hanaa; Uhl, Michael; Appenzeller, Silke; Backofen, Rolf; Sendtner, Michael

    2018-03-20

    Disturbed RNA processing and subcellular transport contribute to the pathomechanisms of motoneuron diseases such as amyotrophic lateral sclerosis and spinal muscular atrophy. RNA-binding proteins are involved in these processes, but the mechanisms by which they regulate the subcellular diversity of transcriptomes, particularly in axons, are not understood. Heterogeneous nuclear ribonucleoprotein R (hnRNP R) interacts with several proteins involved in motoneuron diseases. It is located in axons of developing motoneurons, and its depletion causes defects in axon growth. Here, we used individual nucleotide-resolution cross-linking and immunoprecipitation (iCLIP) to determine the RNA interactome of hnRNP R in motoneurons. We identified ∼3,500 RNA targets, predominantly with functions in synaptic transmission and axon guidance. Among the RNA targets identified by iCLIP, the noncoding RNA 7SK was the top interactor of hnRNP R. We detected 7SK in the nucleus and also in the cytosol of motoneurons. In axons, 7SK localized in close proximity to hnRNP R, and depletion of hnRNP R reduced axonal 7SK. Furthermore, suppression of 7SK led to defective axon growth that was accompanied by axonal transcriptome alterations similar to those caused by hnRNP R depletion. Using a series of 7SK-deletion mutants, we show that the function of 7SK in axon elongation depends on its interaction with hnRNP R but not with the PTEF-B complex involved in transcriptional regulation. These results propose a role for 7SK as an essential interactor of hnRNP R to regulate its function in axon maintenance. Copyright © 2018 the Author(s). Published by PNAS.

  2. Survivin selective inhibitor YM155 induce apoptosis in SK-NEP-1 Wilms tumor cells

    International Nuclear Information System (INIS)

    Tao, Yan-Fang; Wu, Dong; Wang, Na; Feng, Xing; Li, Yan-Hong; Ni, Jian; Wang, Jian; Pan, Jian; Lu, Jun; Du, Xiao-Juan; Sun, Li-Chao; Zhao, Xuan; Peng, Liang; Cao, Lan; Xiao, Pei-Fang; Pang, Li

    2012-01-01

    Survivin, a member of the family of inhibitor of apoptosis proteins, functions as a key regulator of mitosis and programmed cell death. YM155, a novel molecular targeted agent, suppresses survivin, which is overexpressed in many tumor types. The aim of this study was to determine the antitumor activity of YM155 in SK-NEP-1 cells. SK-NEP-1 cell growth in vitro and in vivo was assessed by MTT and nude mice experiments. Annexin V/propidium iodide staining followed by flow cytometric analysis was used to detect apoptosis in cell culture. Then gene expression profile of tumor cells treated with YM155 was analyzed with real-time PCR arrays. We then analyzed the expression data with MEV (Multi Experiment View) cluster software. Datasets representing genes with altered expression profile derived from cluster analyses were imported into the Ingenuity Pathway Analysis tool. YM155 treatment resulted in inhibition of cell proliferation of SK-NEP-1cells in a dose-dependent manner. Annexin V assay, cell cycle, and activation of caspase-3 demonstrates that YM155 induced apoptosis in SK-NEP-1 cells. YM155 significantly inhibited growth of SK-NEP-1 xenografts (YM155 5 mg/kg: 1.45 ± 0.77 cm 3 ; YM155 10 mg/kg: 0.95 ± 0.55 cm 3 ) compared to DMSO group (DMSO: 3.70 ± 2.4 cm 3 ) or PBS group cells (PBS: 3.78 ± 2.20 cm 3 , ANOVA P < 0.01). YM155 treatment decreased weight of tumors (YM155 5 mg/kg: 1.05 ± 0.24 g; YM155 10 mg/kg: 0.72 ± 0.17 g) compared to DMSO group (DMSO: 2.06 ± 0.38 g) or PBS group cells (PBS: 2.36 ± 0.43 g, ANOVA P < 0.01). Real-time PCR array analysis showed between Test group and control group there are 32 genes significantly up-regulated and 54 genes were significantly down-regulated after YM155 treatment. Ingenuity pathway analysis (IPA) showed cell death was the highest rated network with 65 focus molecules and the significance score of 44. The IPA analysis also groups the differentially expressed genes into biological mechanisms that are related to cell

  3. Vypravěčské strategie v povídkách Raymonda Carvera

    OpenAIRE

    SELNER, Ondřej

    2016-01-01

    The thesis Vypravěčské strategie v povídkách Raymonda Carvera deals with the literary concept of a narrator. It's results - typologically different narrators and their characterizations - are based on reading and analysis of all published Carver's short stories. The thesis is divided into two main parts - into the theoretical part in which we briefly introduce the development and fundamental concepts of narrator in the literary theory (e.g. in concepts of F. K. Stanzel, K. Friedmann, G. Genet...

  4. Food truck som ett nytt fenomen i Skåne

    OpenAIRE

    Fromell, Hilda; Winter, Regina

    2016-01-01

    Food truck is a mobile restaurant business which originates from the United States, and can now be seen also in Sweden. There are specific guidelines regarding the food that are served from the food trucks rolling the streets of Skåne from 2013. For example the food should be ecological, which is in keeping with the other municipal goals regarding food and meal. The concept is communicated and marketed through social media, which makes it possible to effectively reach many people. Four food t...

  5. Inclusive K0SK0S resonance production in ep collisions at HERA

    CERN Document Server

    AUTHOR|(CDS)2076592; Magill, S.; Musgrave, B.; Nicholass, D.; Repond, J.; Yoshida, R.; Mattingly, M.C.K.; Antonioli, P.; Bari, G.; Bellagamba, L.; Boscherini, D.; Bruni, A.; Bruni, G.; Cindolo, F.; Corradi, M.; Iacobucci, G.; Margotti, A.; Nania, R.; Polini, A.; Antonelli, S.; Basile, M.; Bindi, M.; Cifarelli, L.; Contin, A.; De Pasquale, S.; Sartorelli, G.; Zichichi, A.; Bartsch, D.; Brock, I.; Hartmann, H.; Hilger, E.; Jakob, H.P.; Jungst, M.; Nuncio-Quiroz, A.E.; Paul, E.; Samson, U.; Schonberg, V.; Shehzadi, R.; Wlasenko, M.; Brook, N.H.; Heath, G.P.; Morris, J.D.; Capua, M.; Fazio, S.; Mastroberardino, A.; Schioppa, M.; Susinno, G.; Tassi, E.; Kim, J.Y.; Ibrahim, Z.A.; Kamaluddin, B.; Wan Abdullah, W.A.T.; Ning, Y.; Ren, Z.; Sciulli, F.; Chwastowski, J.; Eskreys, A.; Figiel, J.; Galas, A.; Gil, M.; Olkiewicz, K.; Stopa, P.; Zawiejski, L.; Adamczyk, L.; Bold, T.; Grabowska-Bold, I.; Kisielewska, D.; Lukasik, J.; Przybycien, M.; Suszycki, L.; Kotanski, A.; Slominski, W.; Behrens, U.; Blohm, C.; Bonato, A.; Borras, K.; Ciesielski, R.; Coppola, N.; Fang, S.; Fourletova, J.; Geiser, A.; Gottlicher, P.; Grebenyuk, J.; Gregor, I.; Haas, T.; Hain, W.; Huttmann, A.; Januschek, F.; Kahle, B.; Katkov, I.I.; Klein, U.; Kotz, U.; Kowalski, H.; Lobodzinska, E.; Lohr, B.; Mankel, R.; Melzer-Pellmann, I.A.; Miglioranzi, S.; Montanari, A.; Namsoo, T.; Notz, D.; Parenti, A.; Rinaldi, L.; Roloff, P.; Rubinsky, I.; Santamarta, R.; Schneekloth, U.; Spiridonov, A.; Szuba, D.; Szuba, J.; Theedt, T.; Wolf, G.; Wrona, K.; Yagues Molina, A.G.; Youngman, C.; Zeuner, W.; Drugakov, V.; Lohmann, W.; Schlenstedt, S.; Barbagli, G.; Gallo, E.; Pelfer, P.G.; Bamberger, A.; Dobur, D.; Karstens, F.; Vlasov, N.N.; Bussey, P.J.; Doyle, A.T.; Dunne, W.; Forrest, M.; Rosin, M.; Saxon, D.H.; Skillicorn, I.O.; Gialas, I.; Papageorgiu, K.; Holm, U.; Klanner, R.; Lohrmann, E.; Schleper, P.; Schorner-Sadenius, T.; Sztuk, J.; Stadie, H.; Turcato, M.; Foudas, C.; Fry, C.; Long, K.R.; Tapper, A.D.; Matsumoto, T.; Nagano, K.; Tokushuku, K.; Yamada, S.; Yamazaki, Y.; Barakbaev, A.N.; Boos, E.G.; Pokrovskiy, N.S.; Zhautykov, B.O.; Aushev, V.; Borodin, M.; Kadenko, I.; Kozulia, A.; Libov, V.; Lisovyi, M.; Lontkovskyi, D.; Makarenko, I.; Sorokin, Iu.; Verbytskyi, A.; Volynets, O.; Son, D.; de Favereau, J.; Piotrzkowski, K.; Barreiro, F.; Glasman, C.; Jimenez, M.; Labarga, L.; del Peso, J.; Ron, E.; Soares, M.; Terron, J.; Zambrana, M.; Corriveau, F.; Liu, C.; Schwartz, J.; Walsh, R.; Zhou, C.; Tsurugai, T.; Antonov, A.; Dolgoshein, B.A.; Gladkov, D.; Sosnovtsev, V.; Stifutkin, A.; Suchkov, S.; Dementiev, R.K.; Ermolov, P.F.; Gladilin, L.K.; Golubkov, Yu.A.; Khein, L.A.; Korzhavina, I.A.; Kuzmin, V.A.; Levchenko, B.B.; Lukina, O.Yu.; Proskuryakov, A.S.; Shcheglova, L.M.; Zotkin, D.S.; Abt, I.; Caldwell, A.; Kollar, D.; Reisert, B.; Schmidke, W.B.; Grigorescu, G.; Keramidas, A.; Koffeman, E.; Kooijman, P.; Pellegrino, A.; Tiecke, H.; Vazquez Acosta, Monica Luisa; Wiggers, L.; Brummer, N.; Bylsma, B.; Durkin, L.S.; Lee, A.; Ling, T.Y.; Allfrey, Philip Daniel; Bell, M.A.; Cooper-Sarkar, A.M.; Devenish, R.C.E.; Ferrando, J.; Foster, B.; Korcsak-Gorzo, K.; Oliver, K.; Robertson, A.; Uribe-Estrada, C.; Walczak, R.; Bertolin, A.; Dal Corso, F.; Dusini, S.; Longhin, A.; Stanco, L.; Bellan, P.; Brugnera, R.; Carlin, R.; Garfagnini, A.; Limentani, S.; Oh, B.Y.; Raval, A.; Ukleja, J.; Whitmore, J.J.; Iga, Y.; D'Agostini, G.; Marini, G.; Nigro, A.; Cole, J.E.; Hart, J.C.; Abramowicz, H.; Ingbir, R.; Kananov, S.; Levy, A.; Stern, A.; Kuze, M.; Maeda, J.; Hori, R.; Kagawa, S.; Okazaki, N.; Shimizu, S.; Tawara, T.; Hamatsu, R.; Kaji, H.; Kitamura, S.; Ota, O.; Ri, Y.D.; Costa, M.; Ferrero, M.I.; Monaco, V.; Sacchi, R.; Solano, A.; Arneodo, M.; Ruspa, M.; Fourletov, S.; Martin, J.F.; Stewart, T.P.; Boutle, S.K.; Butterworth, J.M.; Gwenlan, C.; Jones, T.W.; Loizides, J.H.; Wing, M.; Brzozowska, B.; Ciborowski, J.; Grzelak, G.; Kulinski, P.; Luzniak, P.; Malka, J.; Nowak, R.J.; Pawlak, J.M.; Tymieniecka, T.; Ukleja, A.; zarnecki, A.F.; Adamus, M.; Plucinski, P.; Eisenberg, Y.; Hochman, D.; Karshon, U.; Brownson, E.; Danielson, T.; Everett, A.; Kcira, D.; Reeder, D.D.; Ryan, P.; Savin, A.A.; Smith, W.H.; Wolfe, H.; Bhadra, S.; Catterall, C.D.; Cui, Y.; Hartner, G.; Menary, S.; Noor, U.; Standage, J.; Whyte, J.

    2008-01-01

    Inclusive K0SK0S production in ep collisions at HERA was studied with the ZEUS detector using an integrated luminosity of 0.5 fb-1. Enhancements in the mass spectrum were observed and are attributed to the production of f2(1270)/a02(1320), f'2(1525) and f0(1710). The f0(1710) state was observed with a statistical significance of 5 standard deviations. Masses and widths were obtained using a fit which takes into account theoretical predictions based on SU(3) symmetry arguments.

  6. Differential Cytokine Changes in Patients with Myasthenia Gravis with Antibodies against AChR and MuSK.

    Directory of Open Access Journals (Sweden)

    Vuslat Yilmaz

    Full Text Available Neuromuscular transmission failure in myasthenia gravis (MG is most commonly elicited by autoantibodies (ab to the acetylcholine receptor or the muscle-specific kinase, constituting AChR-MG and MuSK-MG. It is controversial whether these MG subtypes arise through different T helper (Th 1, Th2 or Th17 polarized immune reactions and how these reactions are blunted by immunosuppression. To address these questions, plasma levels of cytokines related to various Th subtypes were determined in patients with AChR-MG, MuSK-MG and healthy controls (CON. Peripheral blood mononuclear cells (PBMC were activated in vitro by anti-CD3, and cytokines were quantified in supernatants. In purified blood CD4+ T cells, RNA of various cytokines, Th subtype specific transcription factors and the co-stimulatory molecule, CD40L, were quantified by qRT-PCR. Plasma levels of Th1, Th2 and Th17 related cytokines were overall not significantly different between MG subtypes and CON. By contrast, in vitro stimulated PBMC from MuSK-MG but not AChR-MG patients showed significantly increased secretion of the Th1, Th17 and T follicular helper cell related cytokines, IFN-γ, IL-17A and IL-21. Stimulated expression of IL-4, IL-6, IL-10 and IL-13 was not significantly different. At the RNA level, expression of CD40L by CD4+ T cells was reduced in both AChR-MG and MuSK-MG patients while expression of Th subset related cytokines and transcription factors were normal. Immunosuppression treatment had two effects: First, it reduced levels of IL12p40 in the plasma of AChR-MG and MuSK-MG patients, leaving other cytokine levels unchanged; second, it reduced spontaneous secretion of IFN-γ and increased secretion of IL-6 and IL-10 by cultured PBMC from AChR-MG, but not MuSK-MG patients. We conclude that Th1 and Th17 immune reactions play a role in MuSK-MG. Immunosuppression attenuates the Th1 response in AChR-MG and MuSK-MG, but otherwise modulates immune responses in AChR-MG and MuSK

  7. Mechanisms underlying the cardiac pacemaker: the role of SK4 calcium-activated potassium channels.

    Science.gov (United States)

    Weisbrod, David; Khun, Shiraz Haron; Bueno, Hanna; Peretz, Asher; Attali, Bernard

    2016-01-01

    The proper expression and function of the cardiac pacemaker is a critical feature of heart physiology. The sinoatrial node (SAN) in human right atrium generates an electrical stimulation approximately 70 times per minute, which propagates from a conductive network to the myocardium leading to chamber contractions during the systoles. Although the SAN and other nodal conductive structures were identified more than a century ago, the mechanisms involved in the generation of cardiac automaticity remain highly debated. In this short review, we survey the current data related to the development of the human cardiac conduction system and the various mechanisms that have been proposed to underlie the pacemaker activity. We also present the human embryonic stem cell-derived cardiomyocyte system, which is used as a model for studying the pacemaker. Finally, we describe our latest characterization of the previously unrecognized role of the SK4 Ca(2+)-activated K(+) channel conductance in pacemaker cells. By exquisitely balancing the inward currents during the diastolic depolarization, the SK4 channels appear to play a crucial role in human cardiac automaticity.

  8. Detection and characterization of side population in Ewing's sarcoma SK-ES-1 cells in vitro

    International Nuclear Information System (INIS)

    Yang, Min; Zhang, Rui; Yan, Ming; Ye, Zhengxu; Liang, Wei; Luo, Zhuojing

    2010-01-01

    Dye exclusion is a valuable technique to isolate cancer stem cells (CSCs) based on an ability of stem cell to efflux fluorescent DNA-binding dye, especially for tumors without unique surface markers. It has been proven that side population (SP) cells that exclude Hoechst 33342 dye are enriched with stem-like cells in several cancer cell lines. In this study, we isolated and characterized SP cells from human Ewing's sarcoma cell line SK-ES-1 in vitro. SP cells were detected in SK-ES-1 and comprised 1.2% of total cell population. Only SP cells had the capacity to regenerate both SP and non-SP cells. The proliferation rates were similar between SP and non-SP cells. However, the clonogenicity and invasiveness of SP cells were significantly higher than that of non-SP cells. Further characterization of this SP phenotype presented other properties. SP cells exhibited increased multi-drug resistance and the ATP binding cassette protein (ABC) transporters were up-regulated in SP population. These findings suggest that SP cells derived from Ewing's sarcoma play the critical role in tumor metastasis and recurrence and might be an ideal target for clinical therapy.

  9. Immunization of mice with LRP4 induces myasthenia similar to MuSK-associated myasthenia gravis.

    Science.gov (United States)

    Mori, Shuuichi; Motohashi, Norio; Takashima, Rumi; Kishi, Masahiko; Nishimune, Hiroshi; Shigemoto, Kazuhiro

    2017-11-01

    Since the first report of experimental animal models of myasthenia gravis (MG) with autoantibodies against low-density lipoprotein receptor-related protein 4 (LRP4), there have not been any major reports replicating the pathogenicity of anti-LRP4 antibodies (Abs). Recent clinical studies have cast doubt on the specificity and pathogenicity of anti-LRP4 antibodies for MG, highlighting the need for further research. In this study, we purified antigens corresponding to the extracellular region of human LRP4 stably expressed with chaperones in 293 cells and used these antigens to immunize female A/J mice. Immunization with LRP4 protein caused mice to develop myasthenia having similar electrophysiological and histological features as are observed in MG patients with circulating Abs against muscle-specific kinase (MuSK). Our results clearly demonstrate that active immunization of mice with LRP4 proteins causes myasthenia similar to the MG induced by anti-MuSK Abs. Further experimental and clinical studies are required to prove the pathogenicity of anti-LRP4 Abs in MG patients. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  10. A dehydrin-dehydrin interaction: the case of SK3 from Opuntia streptacantha

    Directory of Open Access Journals (Sweden)

    Itzell eHernandez

    2014-10-01

    Full Text Available Dehydrins belongs to a large group of highly hydrophilic proteins known as Late Embryogenesis Abundant (LEA proteins. It is well know that dehydrins are intrinsically disordered plant proteins that accumulate during the late stages of embryogenesis and in response to abiotic stresses; however, the molecular mechanisms by which their functions are carried out are still unclear. We have previously reported that transgenic Arabidopsis plants overexpressing an Opuntia streptacantha SK3 dehydrin (OpsDHN1 show enhanced tolerance to freezing stress. Herein, we show using a split-ubiquitin yeast two-hybrid system that OpsDHN1 dimerizes. We found that the deletion of regions containing K-segments and the histidine-rich region in the OpsDHN1 protein affects dimer formation. Not surprisingly, in silico protein sequence analysis suggests that OpsDHN1 is an intrinsically disordered protein, an observation that was confirmed by circular dichroism and gel filtration of the recombinantly expressed protein. The addition of zinc triggered the association of recombinantly expressed OpsDHN1 protein, likely through its histidine-rich motif. These data brings new insights about the molecular mechanism of the OpsDHN1 SK3-dehydrin

  11. Reversal of methylation silencing of Apo2L/TRAIL receptor 1 (DR4) expression overcomes resistance of SK-MEL-3 and SK-MEL-28 melanoma cells to interferons (IFNs) or Apo2L/TRAIL.

    Science.gov (United States)

    Bae, S I; Cheriyath, V; Jacobs, B S; Reu, F J; Borden, E C

    2008-01-17

    Human melanoma cell lines, SK-MEL-3 and SK-MEL-28, despite induction of the proapoptotic cytokine, Apo2L/TRAIL, did not undergo apoptosis in response to interferons (IFN-alpha2b or IFN-beta). Postulating that genes important for apoptosis induction by IFNs might be silenced by methylation, the DNA demethylating agent 5-aza-2'-deoxycytidine (5-AZAdC) was assessed. DR4 (TRAIL-R1) was identified as one of the genes reactivated by 5-AZAdC with a >3-fold increase in 8 of 10 melanoma cell lines. Pretreatment with 5-AZAdC sensitized SK-MEL-3 and SK-MEL-28 cells to apoptosis induced by IFN-alpha2b and IFN-beta; methylation-specific PCR and bisulfite sequencing confirmed demethylation of 5'CpG islands of DR4 and flow cytometry showed an increase in DR4 protein on the cell surface. In cells with reactivated DR4, neutralizing mAB to TRAIL reduced apoptosis in response to IFN-beta or Apo2L/TRAIL. To further confirm the role of DR4, it was expressed by retroviral vector in SK-MEL-3 and SK-MEL-28 cells with reversal of resistance to IFN-beta and Apo2L/TRAIL. Thus, reexpressing DR4 by 5-AZAdC or retroviral transfection in melanoma cell in which promoter methylation had suppressed its expression, potentiated apoptosis by IFN-alpha2b, IFN-beta and Apo2L/TRAIL. Reactivation of silenced proapoptotic genes by inhibitors of DNA methylation may enhance clinical response to IFNs or Apo2L/TRAIL.

  12. Overexpression of YB1 C-terminal domain inhibits proliferation, angiogenesis and tumorigenicity in a SK-BR-3 breast cancer xenograft mouse model.

    Science.gov (United States)

    Shi, Jian-Hong; Cui, Nai-Peng; Wang, Shuo; Zhao, Ming-Zhi; Wang, Bing; Wang, Ya-Nan; Chen, Bao-Ping

    2016-01-01

    Y-box-binding protein 1 (YB1) is a multifunctional transcription factor with vital roles in proliferation, differentiation and apoptosis. In this study, we have examined the role of its C-terminal domain (YB1 CTD) in proliferation, angiogenesis and tumorigenicity in breast cancer. Breast cancer cell line SK-BR-3 was infected with GFP-tagged YB1 CTD adenovirus expression vector. An 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) proliferation assay showed that YB1 CTD decreased SK-BR-3 cell proliferation, and down-regulated cyclin B1 and up-regulated p21 levels in SK-BR-3 cells. YB1 CTD overexpression changed the cytoskeletal organization and slightly inhibited the migration of SK-BR-3 cells. YB1 CTD also inhibited secreted VEGF expression in SK-BR-3 cells, which decreased SK-BR-3-induced EA.hy926 endothelial cell angiogenesis in vitro. YB1 CTD overexpression attenuated the ability of SK-BR-3 cells to form tumours in nude mice, and decreased in vivo VEGF levels and angiogenesis in the xenografts in SK-BR-3 tumour-bearing mice. Taken together, our findings demonstrate the vital role of YB1 CTD overexpression in inhibiting proliferation, angiogenesis and tumorigenicity of breast cancer cell line SK-BR-3.

  13. [Effect of 3-bromopyruvate on mitochondrial membrane potential and apoptosis of human breast carcinoma SK-BR-3 cells].

    Science.gov (United States)

    Zhang, Yuanyuan; Liu, Zhe; Zhang, Qianwen; Chao, Zhenhua; Zhang, Pei; Xia, Fei; Jiang, Chenchen; Liu, Hao; Jiang, Zhiwen

    2013-09-01

    To study the effect of glycolysis inhibitor 3-bromopyruvate (3-BrPA) in inducing apoptosis of human breast carcinoma cells SK-BR-3 and the possible mechanism. MTT assay was used to detect the growth inhibition induced by 3-BrPA in breast cancer cells SK-BR-3. The apoptotic cells were detected by flow cytometry with propidium iodide (PI). ATP levels in the cells were detected by ATP assay kit, and DHE fluorescent probe technique was used to determine superoxide anion levels; the mitochondrial membrane potential was assessed using JC-1 staining assay. MTT assay showed that the proliferation of SK-BR-3 cells was inhibited by 3-BrPA in a time- and concentration-dependent manner. Exposure to 80, 160, and 320 µmol·L(-1) 3-BrPA for 24 h resulted in cell apoptosis rates of 6.7%, 22.3%, and 79.6%, respectively, and the intracellular ATP levels of SK-BR-3 cells treated with 80, 160, 320 µmol·L(-1) 3-BrPA for 5 h were 87.7%, 60.6%, and 23.7% of the control levels. 3-BrPA at 160 µmol·L(-1) increased reactive oxygen levels and lowered mitochondrial membrane potential of SK-BR-3 cells. 3-BrPA can inhibit cell proliferation, reduce the mitochondrial membrane potential and induce apoptosis in SK-BR-3 cells, the mechanism of which may involve a reduced ATP level by inhibiting glycolysis and increasing the reactive oxygen level in the cells.

  14. Experimental analysis on frosting characteristic of SK-type finned refrigerating heat exchanger with large-diameter circular holes

    International Nuclear Information System (INIS)

    Fang, Zhao-song; Wang, Hou-hua; Zhang, Jie; Wu, Wei-wei

    2014-01-01

    This paper presents the construction of both a plane fin-and-tube heat exchanger and a SK-type fin-and-tube heat exchanger. Based on plane fin-and-tube heat exchanger, comparative industrial prototype experiments of SK-type fin-and-tube heat exchanger energy efficiency performance were carried out in the artificial climate chamber. Test results confirmed several findings: when the amount of the refrigerant charged is the same and face velocity u = 3.75 m s −1 , SK-type fin-and-tube heat exchanger refrigeration capacity increases by an average of 9.13%; energy consumption reduces by an average of 11.25%, coefficient of performance (COP) of heat exchanger increases by an average of 22.65% with continuous operation during the first 2 h. Also, when the operation time exceeds 2 h, the COP of both types of heat exchangers are both less than 0.6, illustrating that under frost conditions, the defrost interval should not be too long, otherwise energy consumption may sharply spike. - Highlights: •The large holes of SK-type induced the generation of turbulence flow. •The refrigeration capacity and COP of SK-type exceeds that of plane one. •The SK-type fin-and-tube heat exchanger is a new kind of heat transfer equipment. •The defrost interval should not exceed 2 h under frost conditions

  15. The Making of the Gdańsk Metropolitan Region. Local Discourses of Identities, Powers, and Hopes

    Directory of Open Access Journals (Sweden)

    Czepczyński Mariusz

    2014-12-01

    Full Text Available The process of metropolitanisation of the Gdańsk area is facilitated by public discourse involving local and regional politicians, media, and inhabitants. The discussion is based upon historical narrations, but also local ambitions, hopes and emotions, as well as infrastructural projects and investment attractiveness. Foucault suggests that modern power is a dispersed set of micro-practices, many of which operate through the normalising gaze of surveillance regimes. Gdańsk metropolitan cooperation, competition and encounters make the core of the paper; local and regional unifying initiatives and processes are accompanied by examples of separatism, identity conflicts, and political disagreements

  16. Under the glacier, the groundwater - the case of Skálafell area, Iceland

    Science.gov (United States)

    Vincent, Aude; Hart, Jane

    2017-04-01

    The research addressing glaciers evolution under climate change is well developed, and is now looking not only at their mass balance, but also at the associated subsurface hydrology and downstream hydrology. However, the groundwater component is rarely considered, even though it will be required to forecast the evolution of water resources and of water linked hazards under climate change. The few available studies demonstrate the existence of sub-or pro-glacial aquifers. Some of them suggest strong coupling between rivers and the aquifer, observe the flooding due to water table rising following enhanced glacier melting, or expect stronger recharge in the future due to glacier melting. The present study is the first step of a wider project, GlacAq, aiming at filling this knowledge gap, by characterizing the particular hydrogeology encountered under and downstream of glaciers of alpine type, i.e. sub-, pro- and periglacial hydrogeology, and its sensibility to climate change, in order to provide operational management directions. Skálafell glacier area (Iceland) has been chosen as it has already been followed for climatic, glaciological, and surface hydrology data (Hart et al. (2015), Young et al. (2015)). The present work will use those data, as well as topographic and surface data from the National Land Survey of Iceland, and geological data, to run a comprehensive numerical modelling. The work conducted on the Skálafell site will lead both to the achievement of an operational understanding of a poorly known underground system, and to the anticipation of its hydrodynamic response to climate change. The foreseen mechanisms include an enhanced sub-glacial aquifer recharge, intense surface water bodies-aquifer exchanges, and the aquifer discharge either through springs, or to an offshore system. Those offshore stocks are being increasingly recognised, but their origins are still only guessed at. Skálafell site allows the exploration of the potential role of the

  17. Analysis of CPolSK-based FSO system working in space-to-ground channel

    Science.gov (United States)

    Su, Yuwei; Sato, Takuro

    2018-03-01

    In this article, the transmission performance of a circle polarization shift keying (CPolSK)-based free space optical (FSO) system working in space-to-ground channel is analyzed. Formulas describing the optical polarization distortion caused by the atmospheric turbulence and the communication qualities in terms of signal-to-noise-ratio (SNR), bit-error-ratio (BER) and outage probability of the proposed system are derived. Based on the Stokes parameters data measured by a Japanese optical communication satellite, we evaluate the space-to-ground FSO link and simulate the system performance under a varying regime of turbulence strength. The proposed system provides a more efficient way to compensate scintillation effects in a comparison with the on-off-keying (OOK)-based FSO system. These results are useful to the designing and evaluating of a deep space FSO communication system.

  18. Upper Campanian-Maastrichtian chronostratigraphy of the Skælskør-1 core, Denmark

    DEFF Research Database (Denmark)

    Thibault, Nicolas Rudolph; Anderskouv, Kresten; Bjerager, Morten

    2015-01-01

    The lithostratigraphy, calcareous nannofossil biostratigraphy, carbon- and oxygen-isotope stratigraphy and gamma-ray profile are presented for the Skælskør-1 core, eastern Denmark. The correlation of carbon isotopes to Gubbio (Italy) and ODP Site 762C (Indian Ocean) provides...... the chronostratigrahical framework of the core through a tie to magnetostratigraphy. Two new carbon-isotope excursions are defined for the uppermost Maastrichtian of the core and prove useful for long-distance correlation. Twenty stratigraphic tie-points are used for correlation of the upper Campanian......-Maastrichtian interval by combining carbon-isotope and gamma-ray variations. Significant dissimilarities in the gamma-ray profiles of the Danish Basin cores preclude the sole use of this tool for basin-scale correlations. Bulk oxygen-isotopes and semi-quantitative abundance changes in the warm-water calcareous...

  19. Activation of human IK and SK Ca2+ -activated K+ channels by NS309 (6,7-dichloro-1H-indole-2,3-dione 3-oxime)

    DEFF Research Database (Denmark)

    Strøbaek, Dorte; Teuber, Lene; Jørgensen, Tino D

    2004-01-01

    We have identified and characterized the compound NS309 (6,7-dichloro-1H-indole-2,3-dione 3-oxime) as a potent activator of human Ca2+ -activated K+ channels of SK and IK types, whereas it is devoid of effect on BK type channels. IK- and SK-channels have previously been reported to be activated...

  20. Production of coagulation factor VII in human cell lines Sk-Hep-1 and HKB-11.

    Science.gov (United States)

    Corrêa de Freitas, Marcela Cristina; Bomfim, Aline de Sousa; Mizukami, Amanda; Picanço-Castro, Virgínia; Swiech, Kamilla; Covas, Dimas Tadeu

    2017-09-01

    Recombinant factor VII (rFVII) is the main therapeutic choice for hemophilia patients who have developed inhibitory antibodies against conventional treatments (FVIII and FIX). Because of the post-translational modifications, rFVII needs to be produced in mammalian cell lines. In this study, for the first time, we have shown efficient rFVII production in HepG2, Sk-Hep-1, and HKB-11 cell lines. Experiments in static conditions for a period of 96 h showed that HepG2-FVII produced the highest amounts of rhFVII, with an average of 1843 ng/mL. Sk-hep-1-FVII cells reached a maximum protein production of 1432 ng/mL and HKB-11-FVII cells reached 1468 ng/mL. Sk-Hep-1-rFVII and HKB-11-rFVII were selected for the first step of scale-up. Over 10 days of spinner flask culture, HKB-11 and SK-Hep-1 cells showed a cumulative production of rFVII of 152 μg and 202.6 μg in 50 mL, respectively. Thus, these human cell lines can be used for an efficient production of recombinant FVII. With more investment in basic research, human cell lines can be optimized for the commercial production of different bio therapeutic proteins. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. Involvement of Transglutaminase-2 in α-MSH-Induced Melanogenesis in SK-MEL-2 Human Melanoma Cells.

    Science.gov (United States)

    Kim, Hyun Ji; Lee, Hye Ja; Park, Mi Kyung; Gang, Kyung Jin; Byun, Hyun Jung; Park, Jeong Ho; Kim, Mi Kyung; Kim, Soo Youl; Lee, Chang Hoon

    2014-05-01

    Skin hyperpigmentation is one of the most common skin disorders caused by abnormal melanogenesis. The mechanism and key factors at play are not fully understood. Previous reports have indicated that cystamine (CTM) inhibits melanin synthesis, though its molecular mechanism in melanogenesis remains unclear. In the present study, we investigated the effect of CTM on melanin production using ELISA reader and the expression of proteins involved in melanogenesis by Western blotting, and examined the involvement of transglutaminase-2 (Tgase-2) in SK-MEL-2 human melanoma cells by gene silencing. In the results, CTM dose-dependently suppressed melanin production and dendrite extension in α-MSH-induced melanogenesis of SK-MEL-2 human melanoma cells. CTM also suppressed α-MSH-induced chemotactic migration as well as the expressions of melanogenesis factors TRP-1, TRP-2 and MITF in α-MSH-treated SK-MEL-2 cells. Meanwhile, gene silencing of Tgase-2 suppressed dendrite extension and the expressions of TRP-1 and TRP-2 in α-MSH-treated SK-MEL-2 cells. Overall, these findings suggested that CTM suppresses α-MSH-induced melanogenesis via Tgase-2 inhibition and that therefore, Tgase-2 might be a new target in hyperpigmentation disorder therapy.

  2. Preventive and therapeutic effects of SkQ1-containing Visomitin eye drops against light-induced retinal degeneration.

    Science.gov (United States)

    Novikova, Yu P; Gancharova, O S; Eichler, O V; Philippov, P P; Grigoryan, E N

    2014-10-01

    The human retina is constantly affected by light of varying intensity, this being especially true for photoreceptor cells and retinal pigment epithelium. Traditionally, photoinduced damages of the retina are induced by visible light of high intensity in albino rats using the LIRD (light-induced retinal degeneration) model. This model allows study of pathological processes in the retina and the search for retinoprotectors preventing retinal photodamage. In addition, the etiology and mechanisms of retina damage in the LIRD model have much in common with the mechanisms of the development of age-related retinal disorders, in particular, with age-related macular degeneration (AMD). We have studied preventive and therapeutic effects of Visomitin eye drops (based on the mitochondria-targeted antioxidant SkQ1) on albino rat retinas damaged by bright light. In the first series of experiments, rats receiving Visomitin for two weeks prior to illumination demonstrated significantly less expressed atrophic and degenerative changes in the retina compared to animals receiving similar drops with no SkQ1. In the second series, the illuminated rats were treated for two weeks with Visomitin or similar drops without SkQ1. The damaged retinas of the experimental animals were repaired much more effectively than those of the control animals. Therefore, we conclude that Visomitin SkQ1-containing eye drops have pronounced preventive and therapeutic effects on the photodamaged retina and might be recommended as a photoprotector and a pharmaceutical preparation for the treatment of AMD in combination with conventional medicines.

  3. Effect of SkQ1 Antioxidant on Structural and Functional Conditions of The Brain in PostResuscitation Period

    Directory of Open Access Journals (Sweden)

    M. L. Lovat

    2016-01-01

    Full Text Available The aim was to assess the efficacy of mitochondriatargeted antioxidant SkQ1 in prevention of structural and functional abnormalities of brain postresuscitation after cardiac arrest.Materials and methods. Adult male Wistar rats (n=19 underwent cardiac arrest for 7 minutes followed by resuscitation. Nine rats were administered with 500 nmol/kg SkQ1 per os with water for 2 weeks (1 week before and 1 week after resuscitation. A control group consisted of shamoperated animals (n=10. At days 4—6 post operation locomotor activity and anxiety («elevated plus maze» test and sensorimotor function of limbs («beam walking» test were examined. Total numbers of neurons per 1 mm of their layer length in vulnerable neuronal populations (cerebellar Purkinje cells and piramidal neurons of hippocampus fields CA1 and CA4 were estimated by histological analysis of the specimens stained with cresyl violet on day 7 postresuscitation. To identify possible mechanisms of SkQ1 action, the immunohistochemical study of a glialderived neurotrophic factor (GDNF expression in piramidal neurons of hippocampus was performed by indirect peroxidaseantiperoxidase method and antiGDNF primary polyclonal antibodies.Results. Ischemiareperfusion resulted in neuronal loss in all studied brain areas followed by reduction in locomotor activity and development of sensorimotor deficit. SkQ1 prevented development of postresuscitative locomotor and sensorimotor irregularities, significantly reduced Purkinje cells loss, prevented death of piramidal neurons in hippocampal field CA4, but not in CA1. Data demonstrated, that iIn Purkinje cells from resuscitated rats treated with SkQ1 there was a significant increase in number of GDNFpositive neurons, which were more resistant to ischemia (transition of GDNFnegative cells toward the category of cells actively expressing this factor that promoted their survival postresuscitation.Conclusion. Data confirm the positive effects of SkQ1

  4. Lycopene Inhibits Metastasis of Human Liver Adenocarcinoma SK-Hep-1 Cells by Downregulation of NADPH Oxidase 4 Protein Expression.

    Science.gov (United States)

    Jhou, Bo-Yi; Song, Tuzz-Ying; Lee, Inn; Hu, Miao-Lin; Yang, Nae-Cherng

    2017-08-16

    NADPH oxidase 4 (NOX4), with the sole function to produce reactive oxygen species (ROS), can be a molecular target for disrupting cancer metastasis. Several studies have indicated that lycopene exhibited anti-metastatic actions in vitro and in vivo. However, the role of NOX4 in the anti-metastatic action of lycopene remains unknown. Herein, we first confirmed the anti-metastatic effect of lycopene (0.1-5 μM) on human liver adenocarcinoma SK-Hep-1 cells. We showed that lycopene significantly inhibited NOX4 protein expression, with the strongest inhibition of 64.3 ± 10.2% (P lycopene. Lycopene also significantly inhibited NOX4 mRNA expression, NOX activity, and intracellular ROS levels in SK-Hep-1 cells. We then determined the effects of lycopene on transforming growth factor β (TGF-β)-induced metastasis. We found that TGF-β (5 ng/mL) significantly increased migration, invasion, and adhesion activity, the intracellular ROS level, matrix metalloproteinase 9 (MMP-9) and MMP-2 activities, the level of NOX4 protein expression, and NOX activity. All these TGF-β-induced effects were antagonized by the incubation of SK-Hep-1 cells with lycopene (2.5 μM). Using transient transfection of siRNA against NOX4, we found that the downregulation of NOX4 could mimic lycopene by inhibiting cell migration and the activities of MMP-9 and MMP-2 during the incubation with or without TGF-β on SK-Hep-1 cells. The results demonstrate that the downregulation of NOX4 plays a crucial role in the anti-metastatic action of lycopene in SK-Hep-1 cells.

  5. On the nature of capture centers in irradiated ceramics SK-1

    International Nuclear Information System (INIS)

    Ashurov, M.Kh.; Gasanov, E.M.; Kim, Gen Chan; Nuritdinov, I.; Saidahmedov, Kh.

    2006-01-01

    Full text: Along with other methods, for detection of radiation defects, the luminescent methods are widely used. One of such methods is the thermo-stimulated luminescence (TSL) method. By using this method, information on nature and energy depth (E c ) of capture centers can be obtained. In this work, the influence of high dose γ--irradiation on magnesial ceramics SK-1 was studied by the TL method. The samples were irradiated with ' 60 Co source, and the γ-irradiation dose varied between 10 4 and 10 10 R. The TL curves were measured in the temperature ranges from 80 to 700 K. As a result of irradiation the samples acquired brown colour. After γ-irradiation one can see the peak with maximum of 220 K and the peaks with 428, 548 and 658 K in the low-temperature ( 300 K) regions of the TL curves, respectively. To determine the nature of capture centers the samples were annealed in the air-conditions environment. Annealing conditions do not lead to changes in intensities of the existing TL peaks. After heat treatment in air the increase in intensity of 220, 428 and 548 K of TLS peaks can be observed, whereas in 10-hour treatment the saturation can be observed. Besides, the TL peaks at 353-363 K appears. Consequent restoring annealing decreases intensities of 220, 428 and 548 K and leads to disappearing peaks at 353-363 K. The energy depth values E c calculated by the Uhrbach formula E c =T, K/500 occurred to be : 0,44 eV; 0,86 eV and 1,096 eV for 220, 428 and 548 K, respectively. Besides, at annealing the increase of reflection characteristics of ceramics samples within 300-700 nm in observed, whereas the reflection intensities fall for the samples undergone the annealing. The analysis of the experimental results demonstrated that 220, 428 and 548 K peaks and additional absorption in the region of 300-700 nm are conditioned by V-centers. Possible mechanisms of CC formation and improvement of explanation characteristics of SK-1 ceramics in the ionization fields are

  6. A novel subtilase with NaCl-activated and oxidant-stable activity from Virgibacillus sp. SK37

    Directory of Open Access Journals (Sweden)

    Yamabhai Montarop

    2011-06-01

    Full Text Available Abstract Background Microbial proteases are one of the most commercially valuable enzymes, of which the largest market share has been taken by subtilases or alkaline proteases of the Bacillus species. Despite a large amount of information on microbial proteases, a search for novel proteases with unique properties is still of interest for both basic and applied aspects of this highly complex class of enzymes. Oxidant stable proteases (OSPs have been shown to have a wide application in the detergent and bleaching industries and recently have become one of the most attractive enzymes in various biotechnological applications. Results A gene encoding a novel member of the subtilase superfamily was isolated from Virgibacillus sp. SK37, a protease-producing bacterium isolated from Thai fish sauce fermentation. The gene was cloned by an activity-based screening of a genomic DNA expression library on Luria-Bertani (LB agar plates containing 1 mM IPTG and 3% skim milk. Of the 100,000 clones screened, all six isolated positive clones comprised one overlapping open reading frame of 45% identity to the aprX gene from Bacillus species. This gene, designated aprX-sk37 was cloned into pET21d(+ and over-expressed in E. coli BL21(DE3. The enzyme product, designated AprX-SK37, was purified by an immobilized metal ion affinity chromatography to apparent homogeneity and characterized. The AprX-SK37 enzyme showed optimal catalytic conditions at pH 9.5 and 55°C, based on the azocasein assay containing 5 mM CaCl2. Maximum catalytic activity was found at 1 M NaCl with residual activity of 30% at 3 M NaCl. Thermal stability of the enzyme was also enhanced by 1 M NaCl. The enzyme was absolutely calcium-dependent, with optimal concentration of CaCl2 at 15 mM. Inhibitory effects by phenylmethanesulfonyl fluoride and ethylenediaminetetraacetic acid indicated that this enzyme is a metal-dependent serine protease. The enzyme activity was sensitive towards reducing agents

  7. Expression of the Small Conductance Ca(2+)-Activated Potassium Channel Subtype 3 (SK3) in Rat Uterus after Stimulation with 17β-Estradiol

    DEFF Research Database (Denmark)

    Rahbek, Mette; Nazemi, Sasan; Odum, Lars

    2014-01-01

    the expression of SK3 in the uterus of rats stimulated with 17β-estradiol and progesterone in order to get an in depth understanding of the rat uterine SK3. Using immunohistochemistry SK3 was localized to the glandular and luminal endometrial lamina epitheliali. Furthermore, a weak signal was observed...... in the myometrium. Using Western blot the protein level of SK3 was found to increase in uteri from animals treated with 17β-estradiol, an effect that was not reflected at the mRNA level. The levels of mRNA for SK3 were significantly lower in the uterus of 17β-estradiol-treated animals than in the uterus...... of ovariectomized animals. We conclude that the SK channels are present in the endometrial epithelium, and possibly also in the myometrium of the rat uterus. Furthermore, the hormonal effect on SK3 caused by 17β-estradiol includes divergent regulation at mRNA and protein levels....

  8. TaSK5, an abiotic stress-inducible GSK3/shaggy-like kinase from wheat, confers salt and drought tolerance in transgenic Arabidopsis.

    Science.gov (United States)

    Christov, Nikolai Kirilov; Christova, Petya Koeva; Kato, Hideki; Liu, Yuelin; Sasaki, Kentaro; Imai, Ryozo

    2014-11-01

    A novel cold-inducible GSK3/shaggy-like kinase, TaSK5, was isolated from winter wheat using a macroarray-based differential screening approach. TaSK5 showed high similarity to Arabidopsis subgroup I GSK3/shaggy-like kinases ASK-alpha, AtSK-gamma and ASK-epsilon. RNA gel blot analyses revealed TaSK5 induction by cold and NaCl treatments and to a lesser extent by drought treatment. TaSK5 functionally complemented the cold- and salt-sensitive phenotypes of a yeast GSK3/shaggy-like kinase mutant, △mck1. Transgenic Arabidopsis plants overexpressing TaSK5 cDNA showed enhanced tolerance to salt and drought stresses. By contrast, the tolerance of the transgenic plants to freezing stress was not altered. Microarray analysis revealed that a number of abiotic stress-inducible genes were constitutively induced in the transgenic Arabidopsis plants, suggesting that TaSK5 may function in a novel signal transduction pathway that appears to be unrelated to DREB1/CBF regulon and may involve crosstalk between abiotic and hormonal signals. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  9. Inhibition of SK4 Potassium Channels Suppresses Cell Proliferation, Migration and the Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells.

    Directory of Open Access Journals (Sweden)

    Panshi Zhang

    Full Text Available Treatments for triple-negative breast cancer (TNBC are limited; intermediate-conductance calcium-activated potassium (SK4 channels are closely involved in tumor progression, but little is known about these channels in TNBC. We aimed to investigate whether SK4 channels affect TNBC. First, by immunohistochemistry (IHC and western blotting (WB, increased SK4 protein expression in breast tumor tissues was detected relative to that in non-tumor breast tissues, but there was no apparent expression difference between various subtypes of breast cancer (p>0.05. Next, functional SK4 channels were detected in the TNBC cell line MDA-MB-231 using WB, real-time PCR, immunofluorescence and patch-clamp recording. By employing SK4 specific siRNAs and blockers, including TRAM-34 and clotrimazole, in combination with an MTT assay, a colony-formation assay, flow cytometry and a cell motility assay, we found that the suppression of SK4 channels significantly inhibited cell proliferation and migration and promoted apoptosis in MDA-MB-231 cells (p<0.05. Further investigation revealed that treatment with epidermal growth factor (EGF/basic fibroblast growth factor (bFGF caused MDA-MB-231 cells to undergo the epithelial-mesenchymal transition (EMT and to show increased SK4 mRNA expression. In addition, the down-regulation of SK4 expression inhibited the EMT markers Vimentin and Snail1. Collectively, our findings suggest that SK4 channels are expressed in TNBC and are involved in the proliferation, apoptosis, migration and EMT processes of TNBC cells.

  10. Melanotransferrin induces human melanoma SK-Mel-28 cell invasion in vivo

    International Nuclear Information System (INIS)

    Bertrand, Yanick; Demeule, Michel; Michaud-Levesque, Jonathan; Beliveau, Richard

    2007-01-01

    The expression of melanotransferrin (MTf), a membrane-bound glycoprotein highly expressed in melanomas, is correlated with tumor vascularization and progression, suggesting a proinvasive function associated with MTf in malignant tumors. To test this hypothesis, we silenced MTf in human melanoma SK-MEL-28 cells using small interfering RNA (siRNA) and examined the plasmin activity and invasiveness of MTf-silenced melanoma. In vitro, the siRNA-mediated MTf knockdown inhibited by 58% the cell surface activation of plasminogen into plasmin. In addition, decreased expression of MTf in melanoma cells reduced cell migration. In vivo, we used a nude mice invasion model in which tissue factor (TF) induces vascular [ 125 I]-fibrin deposition following injection. Using this metastasis model, the invasive potential of MTf-silenced cells into the lungs was reduced by fivefold. Altogether, these findings strongly suggest that MTf overexpression in melanoma cells contributes to tumor progession by stimulating plasmin generation as well as cell migration and invasion

  11. Developments in damage assessment by Marie Skłodowska-Curie TRUSS ITN project

    Science.gov (United States)

    González, A.

    2017-05-01

    The growth of cities, the impacts of climate change and the massive cost of providing new infrastructure provide the impetus for TRUSS (Training in Reducing Uncertainty in Structural Safety), a €3.7 million Marie Skłodowska-Curie Action Innovative Training Network project funded by EU’s Horizon 2020 programme, which aims to maximize the potential of infrastructure that already exists (http://trussitn.eu). For that purpose, TRUSS brings together an international, inter-sectoral and multidisciplinary collaboration between five academic and eleven industry institutions from five European countries. The project covers rail and road infrastructure, buildings and energy and marine infrastructure. This paper reports progress in fields such as advanced sensor-based structural health monitoring solutions - unmanned aerial vehicles, optical backscatter reflectometry, monitoring sensors mounted on vehicles, … - and innovative algorithms for structural designs and short- and long-term assessments of buildings, bridges, pavements, ships, ship unloaders, nuclear components and wind turbine towers that will support infrastructure operators and owners in managing their assets.

  12. Metallothionein provides zinc-mediated protective effects against methamphetamine toxicity in SK-N-SH cells.

    Science.gov (United States)

    Ajjimaporn, Amornpan; Swinscoe, John; Shavali, Shaik; Govitrapong, Piyarat; Ebadi, Manuchair

    2005-11-30

    Methamphetamine (METH) is a drug of abuse and neurotoxin that induces Parkinson's-like pathology after chronic usage by targeting dopaminergic neurons. Elucidation of the intracellular mechanisms that underlie METH-induced dopaminergic neuron toxicity may help in understanding the mechanism by which neurons die in Parkinson's disease. In the present study, we examined the role of reactive oxygen species (ROS) in the METH-induced death of human dopaminergic SK-N-SH cells and further assessed the neuroprotective effects of zinc and metallothionein (MT) against METH-induced toxicity in culture. METH significantly increased the production of reactive oxygen species, decreased intracellular ATP levels and reduced the cell viability. Pre-treatment with zinc markedly prevented the loss of cell viability caused by METH treatment. Zinc pre-treatment mainly increased the expression of metallothionein and prevented the generation of reactive oxygen species and ATP depletion caused by METH. Chelation of zinc by CaEDTA caused a significant decrease in MT expression and loss of protective effects of MT against METH toxicity. These results suggest that zinc-induced MT expression protects dopaminergic neurons via preventing the accumulation of toxic reactive oxygen species and halting the decrease in ATP levels. Furthermore, MT may prevent the loss of mitochondrial functions caused by neurotoxins. In conclusion, our study suggests that MT, a potent scavenger of free radicals is neuroprotective against dopaminergic toxicity in conditions such as drug of abuse and in Parkinson's disease.

  13. Variability of morphological conditions of the Vistula river channel in the section Czerwińsk-Kępa Polska

    Directory of Open Access Journals (Sweden)

    Popek Zbigniew

    2015-03-01

    Full Text Available Variability of morphological conditions of the Vistula river channel in the section Czerwińsk-Kępa Polska. The paper presents an assessment of morphological changes that occurred in the Vistula river channel in selected section of about 33 km long, located in the middle of the Vistula river course between Czerwińsk and Kępa Polska (km 576–609. Based on the analysis of archival material from 1972–2009, a characteristics of river channel changes, taking primarily into account the horizontal layout (shape in the plan, was obtained. An attempt was made to estimate the trends and rate of transformation of the following parameters in the river channel: width, length, and position of the riverbanks, abundance and size of islands and fluvial deposits, as well as their percentage in the total area of the main river channel and the floodplain area between levees.

  14. Supplier evaluation system of the wood processing enterprise in the region of the CZ, SK, HU and AT

    OpenAIRE

    Muselíková, Vladislava

    2015-01-01

    The Bachelor thesis Supplier evaluation system of the wood processing enterprise in the region of the CR, the SK, HU and AT deals with the analysis of the current supplier evaluation system in IKEA Industry company in Malacky. The theoretical part is based on literature review and contains a description of the purchasing process and methodology for selection and evaluation of suppliers. The practical part is devoted to the analysis of the Supplier Classification in IKEA. The main output of th...

  15. The first recorded bloom of Pseudochattonella farcimen (Dictyochophyceae, Heterokonta, (Riisberg I., 2008 in the Gulf of Gdańsk

    Directory of Open Access Journals (Sweden)

    Maria Łotocka

    2009-03-01

    Full Text Available In April 2001 a local bloom of the heterokont phytoflagellate Pseudochattonella farcimen (Riisberg I., 2008 (initially named Chattonella aff. verruculosa was observed for the first time in the southern part of the Gulf of Gdańsk.The species occurred in high cell densities: the count was 11.5 × 106 cells dm-3 and the biomass 927.5 µgC dm-3.

  16. Polonium 210Po activities in human blood of patients with ischaemic heart disease from Gda?sk in Poland

    OpenAIRE

    Bory?o, Alicja; Skwarzec, Bogdan; Roma?czyk, Grzegorz; Siebert, Janusz

    2013-01-01

    The determination of polonium 210Po in human blood samples is presented and discussed in this paper. The human blood samples were collected from patients of Medical University of Gda?sk with ischaemic heart disease (morbus ischaemicus cordis, MIC). The polonium concentrations in analyzed human blood samples are very differentiated. 210Po is of particular interest in public health and although is present in the environment in extremely low amounts, it is easily bioaccumulated to the human body...

  17. Calcium-dependent stoichiometries of the KCa2.2 (SK) intracellular domain/calmodulin complex in solution.

    Science.gov (United States)

    Halling, D Brent; Kenrick, Sophia A; Riggs, Austen F; Aldrich, Richard W

    2014-02-01

    Ca(2+) activates SK Ca(2+)-activated K(+) channels through the protein Ca(2+) sensor, calmodulin (CaM). To understand how SK channels operate, it is necessary to determine how Ca(2+) regulates CaM binding to its target on SK. Tagless, recombinant SK peptide (SKp), was purified for binding studies with CaM at low and high Ca(2+) concentrations. Composition gradient multi-angle light scattering accurately measures the molar mass, stoichiometry, and affinity of protein complexes. In 2 mM Ca(2+), SKp and CaM bind with three different stoichiometries that depend on the molar ratio of SKp:CaM in solution. These complexes include 28 kD 1SKp/1CaM, 39 kD 2SKp/1CaM, and 44 kD 1SKp/2CaM. A 2SKp/2CaM complex, observed in prior crystallographic studies, is absent. At sedimentation coefficient is smaller for a 1SKp:1CaM solution than it is for either 2SKp:1CaM or 1SKp:2CaM. At low Ca(2+) and at >100 µM protein concentrations, a molar excess of SKp over CaM causes aggregation. Aggregation is not observed in Ca(2+) or with CaM in molar excess. In low Ca(2+) both 1SKp:1CaM and 1SKp:2CaM solutions have similar sedimentation coefficients, which is consistent with the absence of a 1SKp/2CaM complex in low Ca(2+). These results suggest that complexes with stoichiometries other than 2SKp/2CaM are important in gating.

  18. Recessive variants of MuSK are associated with late onset CMS and predominant limb girdle weakness.

    Science.gov (United States)

    Owen, David; Töpf, Ana; Preethish-Kumar, Veeramani; Lorenzoni, Paulo José; Vroling, Bas; Scola, Rosana Herminia; Dias-Tosta, Elza; Geraldo, Argemiro; Polavarapu, Kiran; Nashi, Saraswati; Cox, Daniel; Evangelista, Teresinha; Dawson, John; Thompson, Rachel; Senderek, Jan; Laurie, Steven; Beltran, Sergi; Gut, Marta; Gut, Ivo; Nalini, Atchayaram; Lochmüller, Hanns

    2018-04-28

    Congenital myasthenic syndrome (CMS) is a heterogeneous disorder that causes fatigable muscle weakness. CMS has been associated with variants in the MuSK gene and, to date, 16 patients have been reported. MuSK-CMS patients present a different phenotypic pattern of limb girdle weakness. Here, we describe four additional patients and discuss the phenotypic and clinical relationship with those previously reported. Two novel damaging missense variants are described: c.1742T > A; p.I581N found in homozygosis, and c.1634T > C; p.L545P found in compound heterozygosis with p.R166*. The reported patients had predominant limb girdle weakness with symptom onset at 12, 17, 18, and 30 years of age, and the majority exhibited a good clinical response to Salbutamol therapy, but not to esterase inhibitors. Meta-analysis including previously reported variants revealed an increased likelihood of a severe, respiratory phenotype with null alleles. Missense variants exclusively affecting the kinase domain, but not the catalytic site, are associated with late onset. These data refine the phenotype associated with MuSK-related CMS. © 2018 Wiley Periodicals, Inc.

  19. Genome-wide analysis of KAP1, the 7SK snRNP complex, and RNA polymerase II

    Directory of Open Access Journals (Sweden)

    Ryan P. McNamara

    2016-03-01

    Full Text Available The transition of RNA polymerase II (Pol II from transcription initiation into productive elongation in eukaryotic cells is regulated by the P-TEFb kinase, which phosphorylates the C-terminal domain of paused Pol II at promoter-proximal regions. Our recent study found that P-TEFb (in an inhibited state bound to the 7SK snRNP complex interacts with the KAP1/TRIM28 transcriptional regulator, and that KAP1 and the 7SK snRNP co-occupy most gene promoters containing paused Pol II. Here we provide a detailed experimental description and analysis of the ChIP-seq datasets that have been deposited into Gene Expression Omnibus (GEO: GS72622, so that independent groups can replicate and expand upon these findings. We propose these datasets would provide valuable information for researchers studying mechanisms of transcriptional regulation including Pol II pausing and pause release. Keywords: P-TEFb/7SK snRNP, KAP1, RNA polymerase II, ChIP-seq, Transcription elongation

  20. Reflection seismic studies over the end-glacial Burträsk fault, Skellefteå, Sweden

    Directory of Open Access Journals (Sweden)

    C. Juhlin

    2011-01-01

    Full Text Available Reflection seismic data were acquired along a ca. 22 km long profile over the end-glacial Burträsk fault with a nominal receiver and source spacing of 20 m. A steeply dipping reflection can be correlated to the Burträsk fault, indicating that the fault dips at about 55° to the southeast near the surface. The reflection from the fault is rather poorly imaged, probably due to a lateral offset in the fault of about 1 km at this location and the crookedness of the seismic profile in the vicinity of the fault. A more pronounced steeply dipping reflection is observed about 4 km southeast of the Burträsk fault. Based on its correlation with a topographic low at the surface this reflection is interpreted to originate from a fracture zone. There are no signs of large displacements along this zone as the glacial ice receded, but earthquakes could be associated with it today. Other reflections on the processed seismic section may originate from changes in lithological variations in the supra-crustal rocks or from intrusions of more mafic rock. Constraints on the fault geometry provided by the reflection seismic data will help determine what stresses were required to activate the fault when the major rupture along it occurred ca. 9500 years ago.

  1. Volcanic history and petrography of the Pliocene Etrüsk Stratovolcano, E Turkey

    Science.gov (United States)

    Oyan, Vural; Keskin, Mehmet; Lebedev, Vladimir; Sharkov, Evgenii; Lustrino, Michele; Mattioli, Michele

    2010-05-01

    The Pliocene Etrusk volcano, with its 3100 m elevation and ~500 km2 area, is one of the major centers of the collision-related volcanism in E Anatolia. It is located in the northeast of Lake Van, sitting almost on the culmination of the "Lake Van dome" structure forming the vertex of the eastern Turkish high plateau (Sengor et al., 2008). A ~5-km-wide horseshoe-shaped caldera, open to the south, is located in the center of the volcano. Apart from two trace element analyses and two K/Ar dates, there are virtually no data available in the literature on this major eruption center. Our study intends to fill this gap with a detailed petrographical, geochemical and geochronological study. Our new K/Ar age determinations indicate that the main volcanic edifice of the Etrusk volcano was formed in period between 4.3 and 3.9 Ma, with the eruption of several intermediate to acid lavas from a central vent. This phase ended up with the formation of a small collapse caldera that produced pyroclastic material emplaced on the earlier lavas. The final impulse of the volcano activity from the Etrusk volcanic center was the eruption of a post-caldera rhyolitic lava flow from the southern flank of the volcano (~3.8-3.7 Ma). After about 2.7 Myr of magmatic quiescence, during the Quaternary time between ~1 and 0.43 Ma, basalts erupted from the SW flank of the Etrusk volcano. They were generated predominantly from a ~N-S extending fissure, as well as from a scoria cone (Karniyarik hill) and a maar-shaped volcanic center (i.e. Düzgeyikçukuru). Edifice-forming products of the Etrüsk stratovolcano are represented by sanidine-plagioclase-biotite-clinopyroxene-phyric trachytes and plagioclase-clinopyroxene-orthopyroxene-phyric trachyandesites containing sporadic olivine phenocrysts. K-feldspar is the most abundant mineral phase in trachitic lavas of the Etrüsk volcanic system. Post caldera lavas, on the other hand, have relatively more evolved compositions ranging from trachydacite to

  2. Hepatotoxicity after liver irradiation in children and adolescents. Results from the RiSK

    Energy Technology Data Exchange (ETDEWEB)

    Roesler, Pascal; Christiansen, Hans [Medical School Hannover, Department of Radiotherapy and Special Oncology, Hannover (Germany); Kortmann, Rolf-Dieter [University of Leipzig, Department of Radiotherapy, Leipzig (Germany); Martini, Carmen [University Hospital of Freiburg, Department of Radiotherapy, Freiburg im Breisgau (Germany); Matuschek, Christiane [Heinrich-Heine University of Duesseldorf, Department of Radiotherapy, Medical Faculty, Duesseldorf (Germany); Meyer, Frank [Hospital Augsburg, Department of Radiotherapy, Augsburg (Germany); Ruebe, Christian [University Hospital of Homburg/Saar, Department of Radiotherapy, Homburg/Saar (Germany); Langer, Thorsten [University Hospital of Schleswig-Holstein, Department of Pediatrics, Pediatric Oncology, Campus Luebeck (Germany); Koch, Raphael [University of Muenster, Institute of Biostatistics and Clinical Research, Muenster (Germany); Eich, Hans Theodor; Willich, Normann [University Hospital of Muenster, Department of Radiotherapy, Muenster (Germany); Steinmann, Diana [Medical School Hannover, Department of Radiotherapy and Special Oncology, Hannover (Germany); University Hospital of Muenster, Department of Radiotherapy, Muenster (Germany)

    2015-05-01

    The aim of this study was to evaluate acute and late radiotherapy-associated hepatotoxicity in consideration of dose-volume effects and liver function in childhood and adolescence. Since 2001, irradiated children and adolescents in Germany have been prospectively documented in the ''Register of Treatment-Associated Late Effects After Radiotherapy of Malignant Diseases in Childhood and Adolescence (RiSK)'' using standardized forms. Toxicity was graded according to the Radiation Therapy Oncology Group (RTOG) criteria. Until April 2012, 1,392 children and adolescents from 62 radiotherapy centers were recruited. In all, 216 patients underwent irradiation of the liver (median age 9 years, range 1-18 years, 70 patients with total-body irradiation, TBI). For 75 % of patients without TBI, information on acute toxicity of the liver was available: 24 patients had acute toxicity of grade 1-4 (grade 1, 2, and 4, in 20, 3, and 1 patient, respectively), including five patients receiving simultaneous hepatotoxic chemotherapy. Information on late toxicity was documented in 465 forms from 216 patients, with a median follow-up of 2 years. A maximum grade of toxicity of ≥ 0 occurred in 18 patients over time (with grade 1, 2, and 3 toxicity occurring in 15, 2, and 1 patient, respectively), including three patients (17 %) with TBI. One of them received simultaneous hepatotoxic chemotherapy. In multivariable analysis, volume-dose correlations showed no statistically noticeable effect on acute or chronic toxicity. Only low hepatotoxicity developed in children after irradiation of various abdominal and thoracic tumors. Due to the low radiation doses to the liver (median liver dose = 5 Gy) and the low toxicities that were consecutively observed, dose-volume curves for liver toxicity could not be established. These findings reflect the cautious attitude of radiation oncologists in terms of attributable liver doses in the treatment of the investigated tumor entities. It

  3. MiRNA-135a regulates the expression of small conductance calcium-activated potassium (SK3) channels in epilepsy-like conditions

    NARCIS (Netherlands)

    Honrath, Birgit; Norwood, Braxton; Tanrioever, Gaye; Kuter, Katarzyna; Henshall, David C; Aksel-Aksoy, Ayla; Schratt, Gerhard; Pasterkamp, Jeroen; Dencher, Norbert A.; Nieweg, Katja; Culmsee, Carsten; Dolga, Amalia Mihalea

    2017-01-01

    Background Excessive and hypersynchronous neuronal discharges are key characteristics in the pathophysiology of neurological disorders such as epilepsy. Owing to their ability of regulating neuronal excitability, small conductance calcium-activated potassium (SK) channels have been implicated in

  4. Pääsküla sõjaväeobjekti jääkreostus ning piirkonna edasise kasutamise perspektiivid / Triin Uudeväli, Anto Raukas

    Index Scriptorium Estoniae

    Uudeväli, Triin

    2004-01-01

    Pääskülas asunud NL-i raketijuhtimisekeskuse ja sideväeosa poolt tekitatud keskkonnareostuse hindamisest, likvideerimisest ning ettepanekutest territooriumi edasiseks kasutamiseks. Kaart. Skeem. Fotod

  5. Arabidopsis mutant sk156 reveals complex regulation of SPL15 in a miR156-controlled gene network.

    Science.gov (United States)

    Wei, Shu; Gruber, Margaret Y; Yu, Bianyun; Gao, Ming-Jun; Khachatourians, George G; Hegedus, Dwayne D; Parkin, Isobel A P; Hannoufa, Abdelali

    2012-09-18

    The Arabidopsis microRNA156 (miR156) regulates 11 members of the SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) family by base pairing to complementary target mRNAs. Each SPL gene further regulates a set of other genes; thus, miR156 controls numerous genes through a complex gene regulation network. Increased axillary branching occurs in transgenic Arabidopsis overexpressing miR156b, similar to that observed in loss-of-function max3 and max4 mutants with lesions in carotenoid cleavage dioxygenases. Arabidopsis miR156b was found to enhance carotenoid levels and reproductive shoot branching when expressed in Brassica napus, suggesting a link between miR156b expression and carotenoid metabolism. However, details of the miR156 regulatory network of SPL genes related to carotenoid metabolism are not known. In this study, an Arabidopsis T-DNA enhancer mutant, sk156, was identified due to its altered branching and trichome morphology and increased seed carotenoid levels compared to wild type (WT) ecovar Columbia. Enhanced miR156b expression due to the 35S enhancers present on the T-DNA insert was responsible for these phenotypes. Constitutive and leaf primodium-specific expression of a miR156-insensitive (mutated) SPL15 (SPL15m) largely restored WT seed carotenoid levels and plant morphology when expressed in sk156. The Arabidopsis native miR156-sensitive SPL15 (SPL15n) and SPL15m driven by a native SPL15 promoter did not restore the WT phenotype in sk156. Our findings suggest that SPL15 function is somewhat redundant with other SPL family members, which collectively affect plant phenotypes. Moreover, substantially decreased miR156b transcript levels in sk156 expressing SPL15m, together with the presence of multiple repeats of SPL-binding GTAC core sequence close to the miR156b transcription start site, suggested feedback regulation of miR156b expression by SPL15. This was supported by the demonstration of specific in vitro interaction between DNA-binding SBP domain of SPL15

  6. Arabidopsis mutant sk156 reveals complex regulation of SPL15 in a miR156-controlled gene network

    Directory of Open Access Journals (Sweden)

    Wei Shu

    2012-09-01

    Full Text Available Abstract Background The Arabidopsis microRNA156 (miR156 regulates 11 members of the SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL family by base pairing to complementary target mRNAs. Each SPL gene further regulates a set of other genes; thus, miR156 controls numerous genes through a complex gene regulation network. Increased axillary branching occurs in transgenic Arabidopsis overexpressing miR156b, similar to that observed in loss-of-function max3 and max4 mutants with lesions in carotenoid cleavage dioxygenases. Arabidopsis miR156b was found to enhance carotenoid levels and reproductive shoot branching when expressed in Brassica napus, suggesting a link between miR156b expression and carotenoid metabolism. However, details of the miR156 regulatory network of SPL genes related to carotenoid metabolism are not known. Results In this study, an Arabidopsis T-DNA enhancer mutant, sk156, was identified due to its altered branching and trichome morphology and increased seed carotenoid levels compared to wild type (WT ecovar Columbia. Enhanced miR156b expression due to the 35S enhancers present on the T-DNA insert was responsible for these phenotypes. Constitutive and leaf primodium-specific expression of a miR156-insensitive (mutated SPL15 (SPL15m largely restored WT seed carotenoid levels and plant morphology when expressed in sk156. The Arabidopsis native miR156-sensitive SPL15 (SPL15n and SPL15m driven by a native SPL15 promoter did not restore the WT phenotype in sk156. Our findings suggest that SPL15 function is somewhat redundant with other SPL family members, which collectively affect plant phenotypes. Moreover, substantially decreased miR156b transcript levels in sk156 expressing SPL15m, together with the presence of multiple repeats of SPL-binding GTAC core sequence close to the miR156b transcription start site, suggested feedback regulation of miR156b expression by SPL15. This was supported by the demonstration of specific in vitro

  7. Comparative Phosphoproteomics Reveals the Role of AmpC β-lactamase Phosphorylation in the Clinical Imipenem-resistant Strain Acinetobacter baumannii SK17.

    Science.gov (United States)

    Lai, Juo-Hsin; Yang, Jhih-Tian; Chern, Jeffy; Chen, Te-Li; Wu, Wan-Ling; Liao, Jiahn-Haur; Tsai, Shih-Feng; Liang, Suh-Yuen; Chou, Chi-Chi; Wu, Shih-Hsiung

    2016-01-01

    Nosocomial infectious outbreaks caused by multidrug-resistant Acinetobacter baumannii have emerged as a serious threat to human health. Phosphoproteomics of pathogenic bacteria has been used to identify the mechanisms of bacterial virulence and antimicrobial resistance. In this study, we used a shotgun strategy combined with high-accuracy mass spectrometry to analyze the phosphoproteomics of the imipenem-susceptible strain SK17-S and -resistant strain SK17-R. We identified 410 phosphosites on 248 unique phosphoproteins in SK17-S and 285 phosphosites on 211 unique phosphoproteins in SK17-R. The distributions of the Ser/Thr/Tyr/Asp/His phosphosites in SK17-S and SK17-R were 47.0%/27.6%/12.4%/8.0%/4.9% versus 41.4%/29.5%/17.5%/6.7%/4.9%, respectively. The Ser-90 phosphosite, located on the catalytic motif S(88)VS(90)K of the AmpC β-lactamase, was first identified in SK17-S. Based on site-directed mutagenesis, the nonphosphorylatable mutant S90A was found to be more resistant to imipenem, whereas the phosphorylation-simulated mutant S90D was sensitive to imipenem. Additionally, the S90A mutant protein exhibited higher β-lactamase activity and conferred greater bacterial protection against imipenem in SK17-S compared with the wild-type. In sum, our results revealed that in A. baumannii, Ser-90 phosphorylation of AmpC negatively regulates both β-lactamase activity and the ability to counteract the antibiotic effects of imipenem. These findings highlight the impact of phosphorylation-mediated regulation in antibiotic-resistant bacteria on future drug design and new therapies. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  8. Comparative Phosphoproteomics Reveals the Role of AmpC β-lactamase Phosphorylation in the Clinical Imipenem-resistant Strain Acinetobacter baumannii SK17*

    Science.gov (United States)

    Lai, Juo-Hsin; Yang, Jhih-Tian; Chern, Jeffy; Chen, Te-Li; Wu, Wan-Ling; Liao, Jiahn-Haur; Tsai, Shih-Feng; Liang, Suh-Yuen; Chou, Chi-Chi

    2016-01-01

    Nosocomial infectious outbreaks caused by multidrug-resistant Acinetobacter baumannii have emerged as a serious threat to human health. Phosphoproteomics of pathogenic bacteria has been used to identify the mechanisms of bacterial virulence and antimicrobial resistance. In this study, we used a shotgun strategy combined with high-accuracy mass spectrometry to analyze the phosphoproteomics of the imipenem-susceptible strain SK17-S and -resistant strain SK17-R. We identified 410 phosphosites on 248 unique phosphoproteins in SK17-S and 285 phosphosites on 211 unique phosphoproteins in SK17-R. The distributions of the Ser/Thr/Tyr/Asp/His phosphosites in SK17-S and SK17-R were 47.0%/27.6%/12.4%/8.0%/4.9% versus 41.4%/29.5%/17.5%/6.7%/4.9%, respectively. The Ser-90 phosphosite, located on the catalytic motif S88VS90K of the AmpC β-lactamase, was first identified in SK17-S. Based on site-directed mutagenesis, the nonphosphorylatable mutant S90A was found to be more resistant to imipenem, whereas the phosphorylation-simulated mutant S90D was sensitive to imipenem. Additionally, the S90A mutant protein exhibited higher β-lactamase activity and conferred greater bacterial protection against imipenem in SK17-S compared with the wild-type. In sum, our results revealed that in A. baumannii, Ser-90 phosphorylation of AmpC negatively regulates both β-lactamase activity and the ability to counteract the antibiotic effects of imipenem. These findings highlight the impact of phosphorylation-mediated regulation in antibiotic-resistant bacteria on future drug design and new therapies. PMID:26499836

  9. The tetraindole SK228 reverses the epithelial-to-mesenchymal transition of breast cancer cells by up-regulating members of the miR-200 family.

    Directory of Open Access Journals (Sweden)

    Chie-Hong Wang

    Full Text Available The results of recent studies have shown that metastasis, the most common malignancy and primary cause of mortality promoted by breast cancer in women, is associated with the epithelial-to-mesenchymal transition (EMT. The results of the current study show that SK228, a novel indole containing substance, exhibits anti-cancer activity. In addition, the effects of SK228 on the regulation of EMT in breast cancer cells as well as the underlying mechanism have been explored. SK228 was observed to induce a fibroblastoid to epithelial-like change in the appearance of various breast cancer cell lines and to suppress the migration and invasion of these cancer cells in vitro. Moreover, expression of E-cadherin was found to increase following SK228 treatment whereas ZEB1 expression was repressed. Expression of other major EMT inducers, including ZEB2, Slug and Twist1, is also repressed by SK228 as a consequence of up-regulation of members of the miR-200 family, especially miR-200c. The results of animal studies demonstrate that SK228 treatment leads to effective suppression of breast cancer growth and metastasis in vivo. The observations made in this investigation show that SK228 reverses the EMT process in breast cancer cells via an effect on the miR-200c/ZEB1/E-cadherin signalling pathway. In addition, the results of a detailed analysis of the in vivo anti-cancer activities of SK228, carried out using a breast cancer xenograft animal model, show that this substance is a potential chemotherapeutic agent for the treatment of breast cancer.

  10. Mitochondria-Targeted Antioxidants SkQ1 and MitoTEMPO Failed to Exert a Long-Term Beneficial Effect in Murine Polymicrobial Sepsis

    Directory of Open Access Journals (Sweden)

    Pia Rademann

    2017-01-01

    Full Text Available Mitochondrial-derived reactive oxygen species have been deemed an important contributor in sepsis pathogenesis. We investigated whether two mitochondria-targeted antioxidants (mtAOX; SkQ1 and MitoTEMPO improved long-term outcome, lessened inflammation, and improved organ homeostasis in polymicrobial murine sepsis. 3-month-old female CD-1 mice (n=90 underwent cecal ligation and puncture (CLP and received SkQ1 (5 nmol/kg, MitoTEMPO (50 nmol/kg, or vehicle 5 times post-CLP. Separately, 52 SkQ1-treated CLP mice were sacrificed at 24 h and 48 h for additional endpoints. Neither MitoTEMPO nor SkQ1 exerted any protracted survival benefit. Conversely, SkQ1 exacerbated 28-day mortality by 29%. CLP induced release of 10 circulating cytokines, increased urea, ALT, and LDH, and decreased glucose but irrespectively of treatment. Similar occurred for CLP-induced lymphopenia/neutrophilia and the NO blood release. At 48 h post-CLP, dying mice had approximately 100-fold more CFUs in the spleen than survivors, but this was not SkQ1 related. At 48 h, macrophage and granulocyte counts increased in the peritoneal lavage but irrespectively of SkQ1. Similarly, hepatic mitophagy was not altered by SkQ1 at 24 h. The absence of survival benefit of mtAOX may be due to the extended treatment and/or a relatively moderate-risk-of-death CLP cohort. Long-term effect of mtAOX in abdominal sepsis appears different to sepsis/inflammation models arising from other body compartments.

  11. The Mitochondria-Targeted Antioxidant SkQ1 Downregulates Aryl Hydrocarbon Receptor-Dependent Genes in the Retina of OXYS Rats with AMD-Like Retinopathy

    Directory of Open Access Journals (Sweden)

    M. L. Perepechaeva

    2014-01-01

    Full Text Available The mitochondria-targeted antioxidant SkQ1 is a novel drug thought to retard development of age-related diseases. It has been shown that SkQ1 reduces clinical signs of retinopathy in senescence-accelerated OXYS rats, which are a known animal model of human age-related macular degeneration (AMD. The aim of this work was to test whether SkQ1 affects transcriptional activity of AhR (aryl hydrocarbon receptor and Nrf2 (nuclear factor erythroid 2-related factor 2, which are considered as AMD-associated genes in the retina of OXYS and Wistar rats. Our results showed that only AhR and AhR-dependent genes were sensitive to SkQ1. Dietary supplementation with SkQ1 decreased the AhR mRNA level in both OXYS and Wistar rats. At baseline, the retinal Cyp1a1 mRNA level was lower in OXYS rats. SkQ1 supplementation decreased the Cyp1a1 mRNA level in Wistar rats, but this level remained unchanged in OXYS rats. Baseline Cyp1a2 and Cyp1b1 mRNA expression was stronger in OXYS than in Wistar rats. In the OXYS strain, Cyp1a2 and Cyp1b1 mRNA levels decreased as a result of SkQ1 supplementation. These data suggest that the Cyp1a2 and Cyp1b1 enzymes are involved in the pathogenesis of AMD-like retinopathy of OXYS rats and are possible therapeutic targets of SkQ1.

  12. Branching fractions of Bplus → Ψ(2S)Kplus and BO → Ψ(2S)K*0 decays at CDF

    International Nuclear Information System (INIS)

    Warburton, A.

    1996-07-01

    This paper describes the observation of the decays B + → ψ(2S)K + and B 0 → ψ(2S)K * (892) 0 in 1.8 TeV pp collisions and presents measurements of the branching fractions Br(B + → ψ(2S)K + ) and Br(B 0 → ψ(2S)K * (892) 0 ) relative to Br(B + → J/ψK + ) and Br(B 0 → J/ψK * (892) 0 ), respectively. The ψ(2S) mesons are reconstructed in both the ψ(2S) → μ + μ - and ψ(2S) → J/ψπ + π - channels. The world average branching fractions for the J/ψ channels are used to extract the absolute branching fractions Br(B + → ψ(2S)K + ) = (6.8±1.0(stat.)±1.4(syst.))x10 -4 and Br(B 0 → ψ(2S)K * (892) 0 ) = (9.0±2.1(stat.)±2. 0(syst.))x10 -4 . 9 refs., 13 figs., 8 tabs

  13. Isoegomaketone induces apoptosis in SK-MEL-2 human melanoma cells through mitochondrial apoptotic pathway via activating the PI3K/Akt pathway.

    Science.gov (United States)

    Kwon, Soon-Jae; Lee, Ju-Hye; Moon, Kwang-Deog; Jeong, Il-Yun; Yee, Sung-Tae; Lee, Mi-Kyung; Seo, Kwon-Il

    2014-11-01

    Isoegomaketone (IK) is a major biologically active component of Perilla frutescens. In this study, we investigated the contribution of reactive oxygen species (ROS) to IK-induced apoptosis in human melanoma SK-MEL-2 cells. We found that IK inhibited the proliferation of SK-MEL-2 human melanoma cells in a dose-dependent manner. IK also induced sub-G1 DNA accumulation, formation of apoptotic bodies, nuclear condensation, and a DNA ladder in SK-MEL-2 cells. IK also induced activation of caspase-3 and -9, whereas caspase‑8 was unaffected. Further, N-acetyl-L-cysteine (NAC, ROS scavenger) treatment to SK-MEL-2 cells significantly reduced IK-induced cell death. Pretreatment of NAC to SK-MEL-2 cells followed by 100 µM IK reduced the protein levels of Bax and cytochrome c as well as PARP cleavage, whereas the protein level of Bcl-2 increased. Moreover, IK inhibited the phosphorylation of AKT/mTOR protein and cell proliferation induced by LY294002, a PI3K inhibitor. In conclusion, IK-induced ROS generation regulates cell growth inhibition and it induces apoptosis through caspase‑dependent and -independent pathways via modulation of PI3K/AKT signaling in SK-MEL-2 cells.

  14. Calotropis procera extract induces apoptosis and cell cycle arrest at G2/M phase in human skin melanoma (SK-MEL-2) cells.

    Science.gov (United States)

    Joshi, Aparna L; Roham, Pratiksha H; Mhaske, Rooth; Jadhav, Mahadev; Krishnadas, Kavitha; Kharat, Amol; Hardikar, Bhagyashree; Kharat, Kiran R

    2015-01-01

    Calotropis procera (family: Asclepiadaceae) contains cardiac glycosides which are cytotoxic to cancer cells. The extracts of C. procera have been reported to be cytotoxic to many cancer cell lines and this is the first report against the human skin melanoma cells (SK-MEL-2). The SK-MEL-2 cells treated with C. procera methanolic extract (CPME) were analysed for growth inhibition and apoptosis. The exposure of phosphatidylserine in apoptotic SK-MEL-2 was analysed by using the Annexin-V FITC flow cytometry method. In CPME-treated SK-MEL-2 cells, 19.6% of apoptotic and 58.3% dead cells were observed. The 15.97% and 15.85% of early apoptotic cells were found at 20 μg/mL of the ouabain and paclitaxel, respectively. Active caspases, nuclear degradation confirmed apoptotic SK-MEL-2 cells in time- and dose-dependent manner. The cell cycle analysis shows that CPME treated cells halt at G2/M phase. Significant cytotoxic activity of CPME against SK-MEL-2 may be attributed to its high cardenolide content.

  15. Biological activity and binding of estradiol to SK-Mel 23 human melanoma cells

    Directory of Open Access Journals (Sweden)

    Sarti M.S.M.V.

    2004-01-01

    Full Text Available Patients expressing estradiol receptors in melanoma cells have been reported to have a better prognosis. We therefore decided to investigate the in vitro effects of ß-estradiol and tamoxifen on the growth and tyrosinase activity of SK-Mel 23 human melanoma cells. Twenty-four-hour treatment with 0.4 nM ß-estradiol inhibited cell proliferation in 30% (0.70 ± 0.03 x 10(5 cells and increased tyrosinase activity in 50% (7130.5 ± 376.5 cpm/10(5 cells, as compared to untreated cells (1.0 ± 0.05 x 10(5 cells and 4769 ± 25.5 cpm/10(5 cells, respectively. Both responses were completely (100% blocked by 1 µM tamoxifen. Higher concentrations (up to 1.6 nM or longer treatments (up to 72 h did not result in a larger effect of the hormone on proliferation or tyrosinase activity. Competition binding assays demonstrated the presence of binding sites to [2,4,6,7-³H]-ß-estradiol, and that the tritiated analogue was displaced by the unlabeled hormone (1 nM to 100 µM, Kd = 0.14 µM, maximal displacement of 93% or by 10 µM tamoxifen (displacement of 60%. ß-estradiol also increased the phosphorylated state of two proteins of 16 and 46 kDa, after 4-h treatment, as determined by Western blot. The absorbance of each band was 1.9- and 4-fold the controls, respectively, as determined with Image-Pro Plus software. Shorter incubation periods with ß-estradiol did not enhance phosporylation; after 6-h treatment with the hormone, the two proteins returned to the control phosphorylation levels. The growth inhibition promoted by estradiol may explain the better prognosis of melanoma-bearing women as compared to men, and open new perspectives for drug therapy.

  16. DJ1 Expression Downregulates in Neuroblastoma Cells (SK-N-MC Chronically Exposed to HIV-1 and Cocaine.

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    Upal eRoy

    2015-07-01

    Full Text Available Background: HIV-associated neurological disorder (HAND has long been recognized as a consequence of Human Immunodeficiency Virus (HIV infection in the brain. The pathology of HAND gets more complicated with the recreational drug use such as cocaine. Recent studies have suggested multiple genetic influences involved in the pathology of addiction and HAND but only a fraction of the entire genetic risk has been investigated so far. In this regard, role of DJ1 protein (a gene linked to autosomal recessive early-onset Parkinson’s disease in regulating dopamine transmission and reactive oxygen species (ROS production in neuronal cells will be worth investigating in HIV-1 and cocaine exposed microenvironment. Being a very abundant protein in the brain, DJ1 could serve as a potential marker for early detection of HIV-1 and/or cocaine related neurological disorder.Methods: In vitro analysis was done to observe the effect of HIV-1 and/or cocaine on DJ1 protein expression in neuroblastoma cells (SK-N-MC. Gene expression and protein analysis of DJ1 was done on the HIV infected and/or cocaine treated SK-N-MC and compared to untreated cells using real time PCR, Western Blot and flow cytometry.Results: Gene expression and protein analysis indicated that there was a significant decrease in DJ1 expression in SK-N-MC chronically exposed to HIV-1 and/or cocaine.Conclusion: This is the first study to establish that DJ1 expression level in the neuronal cells significantly decreased in presence of HIV-1and/or cocaine indicating oxidative stress level of dopamine neurons.

  17. Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin

    Directory of Open Access Journals (Sweden)

    Kuo Y

    2017-04-01

    Full Text Available Yung-Chih Kuo, Chien-Wei Tsao Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China Abstract: A drug delivery system of quercetin (QU-encapsulated liposomes (LS grafted with RMP-7, a bradykinin analog, and lactoferrin (Lf was developed to permeate the blood–brain barrier (BBB and rescue degenerated neurons, acting as an Alzheimer’s disease (AD pharmacotherapy. This colloidal formulation of QU-encapsulated LS grafted with RMP-7 and Lf (RMP-7-Lf-QU-LS was used to traverse human brain microvascular endothelial cells (HBMECs regulated by human astrocytes (HAs and to treat SK-N-MC cells after an insult with cytotoxic β-amyloid (Aβ fibrils. We found that surface RMP-7 and Lf enhanced the ability of QU to cross the BBB without inducing strong toxicity and damaging the tight junction. In addition, RMP-7-Lf-QU-LS significantly reduced Aβ-induced neurotoxicity and improved the viability of SK-N-MC cells. Compared with free QU, RMP-7-Lf-QU-LS could also significantly inhibit the expression of phosphorylated c-Jun N terminal kinase, phosphorylated p38, and phosphorylated tau protein at serine 202 by SK-N-MC cells, indicating an important role of RMP-7, Lf, and LS in protecting neurons against apoptosis. RMP-7-Lf-QU-LS is a promising carrier targeting the BBB to prevent Aβ-insulted neurodegeneration and may have potential in managing AD in future clinical applications. Keywords: Alzheimer’s disease, blood–brain barrier, β-amyloid, drug targeting, neurodegeneration, pharmacotherapy

  18. Upregulation of SK3 and IK1 channels contributes to the enhanced endothelial calcium signaling and the preserved coronary relaxation in obese Zucker rats.

    Directory of Open Access Journals (Sweden)

    Belén Climent

    Full Text Available Endothelial small- and intermediate-conductance KCa channels, SK3 and IK1, are key mediators in the endothelium-derived hyperpolarization and relaxation of vascular smooth muscle and also in the modulation of endothelial Ca2+ signaling and nitric oxide (NO release. Obesity is associated with endothelial dysfunction and impaired relaxation, although how obesity influences endothelial SK3/IK1 function is unclear. Therefore we assessed whether the role of these channels in the coronary circulation is altered in obese animals.In coronary arteries mounted in microvascular myographs, selective blockade of SK3/IK1 channels unmasked an increased contribution of these channels to the ACh- and to the exogenous NO- induced relaxations in arteries of Obese Zucker Rats (OZR compared to Lean Zucker Rats (LZR. Relaxant responses induced by the SK3/IK1 channel activator NS309 were enhanced in OZR and NO- endothelium-dependent in LZR, whereas an additional endothelium-independent relaxant component was found in OZR. Fura2-AM fluorescence revealed a larger ACh-induced intracellular Ca2+ mobilization in the endothelium of coronary arteries from OZR, which was inhibited by blockade of SK3/IK1 channels in both LZR and OZR. Western blot analysis showed an increased expression of SK3/IK1 channels in coronary arteries of OZR and immunohistochemistry suggested that it takes place predominantly in the endothelial layer.Obesity may induce activation of adaptive vascular mechanisms to preserve the dilator function in coronary arteries. Increased function and expression of SK3/IK1 channels by influencing endothelial Ca2+ dynamics might contribute to the unaltered endothelium-dependent coronary relaxation in the early stages of obesity.

  19. Observations of the anisotropy in the cosmic microwave background by the FIRS, SK93, and MSAM-I experiments

    Science.gov (United States)

    Kowitt, Matt; Cheng, Ed; Silverberg, Bob; Ganga, Ken; Page, Lyman; Jarosik, Norm; Netterfield, Barth; Wilkinson, Dave; Meyer, Stephan; Inman, Casey; hide

    1994-01-01

    The observations and results from the FIRS, SK93, and MSAM-1, experiments are discussed. These experiments search for anisotropy in the cosmic microwave background over a range in angular scale from 180 deg to 0.5 deg and a range in frequency from 26 to 680 GHz. Emphasis is placed on the observing strategy and potential systematic errors. Contamination of the data by galactic sources is addressed. Future directions are indicated. The results for all three experiments, as found by us and others, are given in the context of the standard CDM model, Q(sub CDM), and the model-independent band-power estimates.

  20. Składniki mineralne w orzechach występujących w składzie „mieszanek studenckich” = Minerals in nuts which are components of „Trail Mix”

    Directory of Open Access Journals (Sweden)

    Natalia Mikołajczak

    2016-09-01

    Wydział Nauki o Żywności, Uniwersytet Warmińsko-Mazurski w Olsztynie   Uniwersytet Warmińsko-Mazurski w Olsztynie, Katedra Przetwórstwa i Chemii Surowców Roślinnych Pl. Cieszyński 1, 10-726 Olsztyn e-mail: natalia.mikolajczak@uwm.edu.pl   Streszczenie   Wprowadzenie i cel pracy: Orzechy stanowią cenną grupę żywności pod względem wartości żywieniowej, dlatego też są jednym z głównych składników wykorzystywanych w produkcji mieszanek studenckich. Obecność orzechów w codziennej diecie konsumenta ma pozytywny wpływ na organizm poprzez redukcję poziomu cholesterolu we krwi, przeciwdziałanie nowotworom i cukrzycy czy również wspomaganie utraty masy ciała. Wszystkie te korzyści wynikają ze składu chemicznego orzechów i obecności w nich wielu wartościowych składników odżywczych, w szczególności składników mineralnych. Celem pracy był przegląd aktualnego piśmiennictwa na temat zawartości poszczególnych składników mineralnych w orzechach, będących składnikami tradycyjnych mieszanek studenckich, czyli laskowych, włoskich, arachidowych, nerkowcach oraz migdałach. Skrócony opis stanu wiedzy: Orzechy to cenne źródło składników mineralnych, zarówno mikro, jak i makroelementów. Przede wszystkim, orzechy laskowe, włoskie, arachidowe oraz migdały dostarczają znacznych ilości potasu, którego zawartość w 100 g surowca odnotowuje się na poziomie wyższym niż 500 mg. Z kolei zawartość fosforu obecna jest na zbliżonym poziomie jedynie w migdałach. Mikroelementy takie jak żelazo, miedź, cynk i mangan występują we wszystkich orzechach w ilości 5-10 mg/100 g. Podsumowanie: Zebrane dane literaturowe potwierdzają fakt, że analizowane rodzaje orzechów są cennym źródłem zarówno mikro, jak i makroelementów. Są one bogactwem potasu, fosforu, magnezu oraz wapnia. Ponadto, dostarczają także znacznych ilości żelaza, cynku oraz manganu.   Słowa kluczowe: składniki mineralne, orzechy laskowe, orzechy

  1. The SigmaR1 chaperone drives breast and colorectal cancer cell migration by tuning SK3-dependent Ca2+ homeostasis.

    Science.gov (United States)

    Gueguinou, M; Crottès, D; Chantôme, A; Rapetti-Mauss, R; Potier-Cartereau, M; Clarysse, L; Girault, A; Fourbon, Y; Jézéquel, P; Guérin-Charbonnel, C; Fromont, G; Martin, P; Pellissier, B; Schiappa, R; Chamorey, E; Mignen, O; Uguen, A; Borgese, F; Vandier, C; Soriani, O

    2017-06-22

    The remodeling of calcium homeostasis contributes to the cancer hallmarks and the molecular mechanisms involved in calcium channel regulation in tumors remain to be characterized. Here, we report that SigmaR1, a stress-activated chaperone, is required to increase calcium influx by triggering the coupling between SK3, a Ca 2+ -activated K + channel (KCNN3) and the voltage-independent calcium channel Orai1. We show that SigmaR1 physically binds SK3 in BC cells. Inhibition of SigmaR1 activity, either by molecular silencing or by the use of sigma ligand (igmesine), decreased SK3 current and Ca 2+ entry in breast cancer (BC) and colorectal cancer (CRC) cells. Interestingly, SigmaR1 inhibition diminished SK3 and/or Orai1 levels in lipid nanodomains isolated from BC cells. Analyses of tissue microarray from CRC patients showed higher SigmaR1 expression levels in cancer samples and a correlation with tumor grade. Moreover, the exploration of a cohort of 4937 BC patients indicated that high expression of SigmaR1 and Orai1 channels was significantly correlated to a lower overall survival. As the SK3/Orai1 tandem drives invasive process in CRC and bone metastasis progression in BC, our results may inaugurate innovative therapeutic approaches targeting SigmaR1 to control the remodeling of Ca 2+ homeostasis in epithelial cancers.

  2. Dílna kultury zvoncovitých pohárů na zpracování rohovce typu Stránská skála (Brno, katastrální území Slatina)

    Czech Academy of Sciences Publication Activity Database

    Šebela, Lubomír; Škrdla, Petr; Přichystal, A.; Kopacz, J.

    2016-01-01

    Roč. 1, č. 1 (2016), s. 119-128 ISSN 2453-8612 Institutional support: RVO:68081758 Keywords : Moravia (East part of Czech Republic) * Stránská skála (Brno, cadastral territory of Slatina) * Bell Beaker culture * workshop * chert of the Stránská skála type Subject RIV: AC - Archeology, Anthropology, Ethnology https://fphil.uniba.sk/fileadmin/fif/katedry_pracoviska/karch/MusArch/1_1/119-128.pdf

  3. Effect of the SK/IK channel modulator 4,5-dichloro-1,3-diethyl-1,3-dihydro-benzoimidazol-2-one (NS4591) on contractile force in rat, pig and human detrusor smooth muscle

    DEFF Research Database (Denmark)

    Nielsen, Jens Steen; Rode, Frederik; Rahbek, Mette

    2011-01-01

    • To investigate the importance of small (SK)- and intermediate (IK)-conductance Ca2(+) -activated K(+) channels on bladder function, by studying the effects of 4,5-dichloro-1,3-diethyl-1,3-dihydro-benzoimidazol-2-one (NS4591), a new modulator of SK/IK channels, on contractions induced by electri......• To investigate the importance of small (SK)- and intermediate (IK)-conductance Ca2(+) -activated K(+) channels on bladder function, by studying the effects of 4,5-dichloro-1,3-diethyl-1,3-dihydro-benzoimidazol-2-one (NS4591), a new modulator of SK/IK channels, on contractions induced...

  4. Rapid agonist-induced loss of sup 125 I-. beta. -endorphin opioid receptor sites in NG108-15, but not SK-N-SH neuroblastoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Cone, R.I.; Lameh, J.; Sadee, W. (Univ. of California, San Francisco (United States))

    1991-01-01

    The authors have measured {mu} and {delta} opioid receptor sites on intact SK-N-SH and NG108-15 neuroblastoma cells, respectively, in culture. Use of {sup 125}I-{beta}-endorphin ({beta}E) as a tracer, together with {beta}E(6-31) to block high-affinity non-opioid binding in both cell lines, permitted the measurement of cell surface {mu} and {delta} opioid receptor sites. Labeling was at {delta} sites in NG108-15 cells and predominantly at {mu} sites in SK-N-SH cells. Pretreatment with the {mu} and {delta} agonist, DADLE, caused a rapid loss of cell surface {delta} receptor sites in NG108-15 cells, but failed to reduce significantly {mu} receptor density in SK-N-SH cells.

  5. The cytotoxicity of a 2-chloroethylnitrosourea analog of sarcosinamide in the SK-MG-1 human glioma cell line as a possible indicator for transport

    Energy Technology Data Exchange (ETDEWEB)

    Skalski, V.; Feindel, W.; Panasci, L.C. (Lady Davis Institute for Medical Research, Montreal, Quebec (Canada))

    1989-07-01

    The cytotoxicities of a new sarcosinamide analog of chloroethylnitrosourea (SarCNU) and of BCNU were examined in the glioma cell line SK-MG-1 in the presence or absence of excess concentrations of amino acids and sarcosinamide. The cytotoxicity of SarCNU, but not of BCNU, was significantly reduced in the presence of excess sarcosinamide. The stability of SarCNU was not significantly altered by increasing concentrations of sarcosinamide. In order to investigate the possibility that sarcosinamide inhibits the uptake of SarCNU, the transport of tritiated sarcosinamide was examined in SK-MG-1 cells. The uptake of 3H-sarcosinamide was inhibited by excess, unlabelled sarcosinamide and SarCNU but not by BCNU, glycine or sarcosine. These results suggest the existence of a carrier-mediated transport for sarcosinamide which can accomodate SarCNU in SK-MG-1 cells.

  6. Chronic ethanol exposure induces SK-N-SH cell apoptosis by increasing N-methyl-D-aspartic acid receptor expression and intracellular calcium.

    Science.gov (United States)

    Wang, Hongbo; Wang, Xiaolong; Li, Yan; Yu, Hao; Wang, Changliang; Feng, Chunmei; Xu, Guohui; Chen, Jiajun; You, Jiabin; Wang, Pengfei; Wu, Xu; Zhao, Rui; Zhang, Guohua

    2018-04-01

    It has been identified that chronic ethanol exposure damages the nervous system, particularly neurons. There is scientific evidence suggesting that neuronal loss caused by chronic ethanol exposure has an association with neuron apoptosis and intracellular calcium oscillation is one of the primary inducers of apoptosis. Therefore, the present study aimed to investigate the inductive effects of intracellular calcium oscillation on apoptosis in SK-N-SH human neuroblastoma cells and the protective effects of the N-methyl-D-aspartic acid receptor (NMDAR) antagonist, memantine, on SK-N-SH cell apoptosis caused by chronic ethanol exposure. SK-N-SH cells were treated with 100 mM ethanol and memantine (4 µM) for 2 days. Protein expression of NR1 was downregulated by RNA interference (RNAi). Apoptosis was detected by Annexin V/propidium iodide (PI) double-staining and flow cytometry and cell viability was detected using an MTS kit. Fluorescence dual wavelength spectrophotometry was used to determine the intracellular calcium concentration and the levels of NR1 and caspase-3 were detected using western blotting. NR1 mRNA levels were also detected using qPCR. It was found that chronic ethanol exposure reduced neuronal cell viability and caused apoptosis of SK-N-SH cells, and the extent of damage in SK-N-SH cells was associated with ethanol exposure concentration and time. In addition, chronic ethanol exposure increased the concentration of intracellular calcium in SK-N-SH cells by inducing the expression of NMDAR, resulting in apoptosis, and memantine treatment reduced ethanol-induced cell apoptosis. The results of the present study indicate that the application of memantine may provide a novel strategy for the treatment of alcoholic dementia.

  7. In vitro characterization of PlySK1249, a novel phage lysin, and assessment of its antibacterial activity in a mouse model of Streptococcus agalactiae bacteremia.

    Science.gov (United States)

    Oechslin, Frank; Daraspe, Jean; Giddey, Marlyse; Moreillon, Philippe; Resch, Grégory

    2013-12-01

    Beta-hemolytic Streptococcus agalactiae is the leading cause of bacteremia and invasive infections. These diseases are treated with β-lactams or macrolides, but the emergence of less susceptible and even fully resistant strains is a cause for concern. New bacteriophage lysins could be promising alternatives against such organisms. They hydrolyze the bacterial peptidoglycan at the end of the phage cycle, in order to release the phage progeny. By using a bioinformatic approach to screen several beta-hemolytic streptococci, a gene coding for a lysin was identified on a prophage carried by Streptococcus dysgalactiae subsp. equisimilis SK1249. The gene product, named PlySK1249, harbored an original three-domain structure with a central cell wall-binding domain surrounded by an N-terminal amidase and a C-terminal CHAP domain. Purified PlySK1249 was highly lytic and bactericidal for S. dysgalactiae (2-log10 CFU/ml decrease within 15 min). Moreover, it also efficiently killed S. agalactiae (1.5-log10 CFU/ml decrease within 15 min) but not several streptococcal commensal species. We further investigated the activity of PlySK1249 in a mouse model of S. agalactiae bacteremia. Eighty percent of the animals (n = 10) challenged intraperitoneally with 10(6) CFU of S. agalactiae died within 72 h, whereas repeated injections of PlySK1249 (45 mg/kg 3 times within 24 h) significantly protected the mice (P S. dysgalactiae, demonstrated high cross-lytic activity against S. agalactiae both in vitro and in vivo. These encouraging results indicated that PlySK1249 might represent a good candidate to be developed as a new enzybiotic for the treatment of systemic S. agalactiae infections.

  8. RuvBL2 Is Involved in Histone Deacetylase Inhibitor PCI-24781-Induced Cell Death in SK-N-DZ Neuroblastoma Cells

    Science.gov (United States)

    Zhan, Qinglei; Tsai, Sauna; Lu, Yonghai; Wang, Chunmei; Kwan, Yiuwa; Ngai, Saiming

    2013-01-01

    Neuroblastoma is the second most common solid tumor diagnosed during infancy. The survival rate among children with high-risk neuroblastoma is less than 40%, highlighting the urgent needs for new treatment strategies. PCI-24781 is a novel hydroxamic acid-based histone deacetylase (HDAC) inhibitor that has high efficacy and safety for cancer treatment. However, the underlying mechanisms of PCI-24781 are not clearly elucidated in neuroblastoma cells. In the present study, we demonstrated that PCI-24781 treatment significantly inhibited tumor growth at very low doses in neuroblastoma cells SK-N-DZ, not in normal cell line HS-68. However, PCI-24781 caused the accumulation of acetylated histone H3 both in SK-N-DZ and HS-68 cell line. Treatment of SK-N-DZ with PCI-24781 also induced cell cycle arrest in G2/M phase and activated apoptosis signaling pathways via the up-regulation of DR4, p21, p53 and caspase 3. Further proteomic analysis revealed differential protein expression profiles between non-treated and PCI-24781 treated SK-N-DZ cells. Totally 42 differentially expressed proteins were identified by MALDI-TOF MS system. Western blotting confirmed the expression level of five candidate proteins including prohibitin, hHR23a, RuvBL2, TRAP1 and PDCD6IP. Selective knockdown of RuvBL2 rescued cells from PCI-24781-induced cell death, implying that RuvBL2 might play an important role in anti-tumor activity of PCI-24781 in SK-N-DZ cells. The present results provide a new insight into the potential mechanism of PCI-24781 in SK-N-DZ cell line. PMID:23977108

  9. Polonium 210Po activities in human blood of patients with ischaemic heart disease from Gdańsk in Poland.

    Science.gov (United States)

    Boryło, Alicja; Skwarzec, Bogdan; Romańczyk, Grzegorz; Siebert, Janusz

    The determination of polonium 210 Po in human blood samples is presented and discussed in this paper. The human blood samples were collected from patients of Medical University of Gdańsk with ischaemic heart disease ( morbus ischaemicus cordis , MIC ). The polonium concentrations in analyzed human blood samples are very differentiated. 210 Po is of particular interest in public health and although is present in the environment in extremely low amounts, it is easily bioaccumulated to the human body. The study shows that the amount of 210 Po that is incorporated into the human body depends on the food habits and some difference in its levels could be observed between smokers and non-smokers.

  10. Impact of image segmentation on high-content screening data quality for SK-BR-3 cells

    Directory of Open Access Journals (Sweden)

    Li Yizheng

    2007-09-01

    Full Text Available Abstract Background High content screening (HCS is a powerful method for the exploration of cellular signalling and morphology that is rapidly being adopted in cancer research. HCS uses automated microscopy to collect images of cultured cells. The images are subjected to segmentation algorithms to identify cellular structures and quantitate their morphology, for hundreds to millions of individual cells. However, image analysis may be imperfect, especially for "HCS-unfriendly" cell lines whose morphology is not well handled by current image segmentation algorithms. We asked if segmentation errors were common for a clinically relevant cell line, if such errors had measurable effects on the data, and if HCS data could be improved by automated identification of well-segmented cells. Results Cases of poor cell body segmentation occurred frequently for the SK-BR-3 cell line. We trained classifiers to identify SK-BR-3 cells that were well segmented. On an independent test set created by human review of cell images, our optimal support-vector machine classifier identified well-segmented cells with 81% accuracy. The dose responses of morphological features were measurably different in well- and poorly-segmented populations. Elimination of the poorly-segmented cell population increased the purity of DNA content distributions, while appropriately retaining biological heterogeneity, and simultaneously increasing our ability to resolve specific morphological changes in perturbed cells. Conclusion Image segmentation has a measurable impact on HCS data. The application of a multivariate shape-based filter to identify well-segmented cells improved HCS data quality for an HCS-unfriendly cell line, and could be a valuable post-processing step for some HCS datasets.

  11. A High Molecular-Mass Anoxybacillus sp. SK3-4 Amylopullulanase: Characterization and Its Relationship in Carbohydrate Utilization

    Directory of Open Access Journals (Sweden)

    Kian Mau Goh

    2013-05-01

    Full Text Available An amylopullulanase of the thermophilic Anoxybacillus sp. SK3-4 (ApuASK was purified to homogeneity and characterized. Though amylopullulanases larger than 200 kDa are rare, the molecular mass of purified ApuASK appears to be approximately 225 kDa, on both SDS-PAGE analyses and native-PAGE analyses. ApuASK was stable between pH 6.0 and pH 8.0 and exhibited optimal activity at pH 7.5. The optimal temperature for ApuASK enzyme activity was 60 °C, and it retained 54% of its total activity for 240 min at 65 °C. ApuASK reacts with pullulan, starch, glycogen, and dextrin, yielding glucose, maltose, and maltotriose. Interestingly, most of the previously described amylopullulanases are unable to produce glucose and maltose from these substrates. Thus, ApuASK is a novel, high molecular-mass amylopullulanase able to produce glucose, maltose, and maltotriose from pullulan and starch. Based on whole genome sequencing data, ApuASK appeared to be the largest protein present in Anoxybacillus sp. SK3-4. The α-amylase catalytic domain present in all of the amylase superfamily members is present in ApuASK, located between the cyclodextrin (CD-pullulan-degrading N-terminus and the α-amylase catalytic C-terminus (amyC domains. In addition, the existence of a S-layer homology (SLH domain indicates that ApuASK might function as a cell-anchoring enzyme and be important for carbohydrate utilization in a streaming hot spring.

  12. Anthocyanins Protect SK-N-SH Cells Against Acrolein-Induced Toxicity by Preserving the Cellular Redox State.

    Science.gov (United States)

    Belkacemi, Abdenour; Ramassamy, Charles

    2016-01-01

    In Alzheimer's disease (AD) and in mild cognitive impairment (MCI) patients, by-products of lipid peroxidation such as acrolein accumulated in vulnerable regions of the brain. We have previously shown that acrolein is a highly reactive and neurotoxic aldehyde and its toxicity involves the alteration of several redox-sensitive pathways. Recently, protein-conjugated acrolein in cerebrospinal fluid has been proposed as a biomarker to distinguish between MCI and AD. With growing evidence of the early involvement of oxidative stress in AD etiology, one would expect that a successful therapy should prevent brain oxidative damage. In this regard, several studies have demonstrated that polyphenol-rich extracts exert beneficial effect on cognitive impairment and oxidative stress. We have recently demonstrated the efficacy of an anthocyanin formulation (MAF14001) against amyloid-β-induced oxidative stress. The aim of this study is to investigate the neuroprotective effect of MAF14001 as a mixture of anthocyanins, a particular class of polyphenols, against acrolein-induced oxidative damage in SK-N-SH neuronal cells. Our results demonstrated that MAF14001, from 5μM, was able to efficiently protect SK-N-SH cells against acrolein-induced cell death. MAF14001 was able to lower reactive oxygen species and protein carbonyl levels induced by acrolein. Moreover, MAF1401 prevented glutathione depletion and positively modulated, in the presence of acrolein, some oxidative stress-sensitive pathways including the transcription factors NF-κB and Nrf2, the proteins γ-GCS and GSK3β, and the protein adaptator p66Shc. Along with its proven protective effect against amyloid-β toxicity, these results demonstrate that MAF14001 could target multiple mechanisms and could be a promising agent for AD prevention.

  13. Neuronal uptake and neuroprotective effect of curcumin-loaded PLGA nanoparticles on the human SK-N-SH cell line.

    Science.gov (United States)

    Doggui, Sihem; Sahni, Jasjeet Kaur; Arseneault, Madeleine; Dao, Lé; Ramassamy, Charles

    2012-01-01

    Curcumin, a natural polyphenolic pigment present in the spice turmeric (Curcuma longa), is known to possess a pleiotropic activity such as antioxidant, anti-inflammatory, and anti-amyloid-β activities. However, these benefits of curcumin are limited by its poor aqueous solubility and oral bioavailability. In the present study, a polymer-based nanoparticle approach has been utilized to deliver drugs to neuronal cells. Curcumin was encapsulated in biodegradable poly (lactide-co-glycolide) (PLGA) based-nanoparticulate formulation (Nps-Cur). Dynamic laser light scattering and transmission electronic microscopy analysis indicated a particle diameter ranging from 80 to 120 nm. The entrapment efficiency was 31% with 15% drug-loading. In vitro release kinetics of curcumin from Nps-Cur revealed a biphasic pattern with an initial exponential phase followed by a slow release phase. Cellular internalization of Nps-Cur was confirmed by fluorescence and confocal microscopy with a wide distribution of the fluorescence in the cytoplasm and within the nucleus. The prepared nanoformulation was characterized for cellular toxicity and biological activity. Cytotoxicity assays showed that void PLGA-nanoparticles (Nps) and curcumin-loaded PLGA nanoparticles (Nps-Cur) were nontoxic to human neuroblastoma SK-N-SH cells. Moreover, Nps-Cur was able to protect SK-N-SH cells against H2O2 and prevent the elevation of reactive oxygen species and the consumption of glutathione induced by H2O2. Interestingly, Nps-Cur was also able to prevent the induction of the redox-sensitive transcription factor Nrf2 in the presence of H2O2. Taken together, these results suggest that Nps-Cur could be a promising drug delivery strategy to protect neurons against oxidative damage as observed in Alzheimer's disease.

  14. Effect of the SK/IK channel modulator 4,5-dichloro-1,3-diethyl-1,3-dihydro-benzoimidazol-2-one (NS4591) on contractile force in rat, pig and human detrusor smooth muscle

    DEFF Research Database (Denmark)

    Nielsen, Jens Steen; Rode, Frederik; Rahbek, Mette

    2011-01-01

    • To investigate the importance of small (SK)- and intermediate (IK)-conductance Ca2(+) -activated K(+) channels on bladder function, by studying the effects of 4,5-dichloro-1,3-diethyl-1,3-dihydro-benzoimidazol-2-one (NS4591), a new modulator of SK/IK channels, on contractions induced by electri...

  15. Kit-negative fibroblast-like cells expressing SK3, a Ca2+-activated K+ channel, in the gut musculature in health and disease

    DEFF Research Database (Denmark)

    Vanderwinden, Jean-Marie; Rumessen, Jüri J; de Kerchove d'Exaerde, Alban

    2002-01-01

    The apamin-sensitive component of the inhibitory response of the gastrointestinal musculature involves the small conductance Ca(2+)-activated K(+) channel SK3. Kit-immunoreactive (ir) interstitial cells of Cajal appear to be involved in nitrergic inhibition while the role of the recently describe...

  16. $K^{0}_{s}-K^{0}_{s}$ correlations in pp collisions at $\\sqrt{s}=7 TeV$ from the LHC ALICE experiment

    CERN Document Server

    Abelev, Betty; Adamova, Dagmar; Adare, Andrew Marshall; Aggarwal, Madan; Aglieri Rinella, Gianluca; Agocs, Andras Gabor; Agostinelli, Andrea; Aguilar Salazar, Saul; Ahammed, Zubayer; Ahmad, Arshad; Ahmad, Nazeer; Ahn, Sang Un; Akindinov, Alexander; Aleksandrov, Dmitry; Alessandro, Bruno; Alfaro Molina, Jose Ruben; Alici, Andrea; Alkin, Anton; Almaraz Avina, Erick Jonathan; Alme, Johan; Alt, Torsten; Altini, Valerio; Altinpinar, Sedat; Altsybeev, Igor; Andrei, Cristian; Andronic, Anton; Anguelov, Venelin; Anielski, Jonas; Anticic, Tome; Antinori, Federico; Antonioli, Pietro; Aphecetche, Laurent Bernard; Appelshauser, Harald; Arbor, Nicolas; Arcelli, Silvia; Armesto, Nestor; Arnaldi, Roberta; Aronsson, Tomas Robert; Arsene, Ionut Cristian; Arslandok, Mesut; Augustinus, Andre; Averbeck, Ralf Peter; Awes, Terry; Aysto, Juha Heikki; Azmi, Mohd Danish; Bach, Matthias Jakob; Badala, Angela; Baek, Yong Wook; Bailhache, Raphaelle Marie; Bala, Renu; Baldini Ferroli, Rinaldo; Baldisseri, Alberto; Baldit, Alain; Baltasar Dos Santos Pedrosa, Fernando; Ban, Jaroslav; Baral, Rama Chandra; Barbera, Roberto; Barile, Francesco; Barnafoldi, Gergely Gabor; Barnby, Lee Stuart; Barret, Valerie; Bartke, Jerzy Gustaw; Basile, Maurizio; Bastid, Nicole; Basu, Sumit; Bathen, Bastian; Batigne, Guillaume; Batyunya, Boris; Baumann, Christoph Heinrich; Bearden, Ian Gardner; Beck, Hans; Behera, Nirbhay Kumar; Belikov, Iouri; Bellini, Francesca; Bellwied, Rene; Belmont-Moreno, Ernesto; Bencedi, Gyula; Beole, Stefania; Berceanu, Ionela; Bercuci, Alexandru; Berdnikov, Yaroslav; Berenyi, Daniel; Bergognon, Anais Annick Erica; Berzano, Dario; Betev, Latchezar; Bhasin, Anju; Bhati, Ashok Kumar; Bhom, Jihyun; Bianchi, Livio; Bianchi, Nicola; Bianchin, Chiara; Bielcik, Jaroslav; Bielcikova, Jana; Bilandzic, Ante; Bjelogrlic, Sandro; Blanco, F; Blanco, Francesco; Blau, Dmitry; Blume, Christoph; Bock, Nicolas; Boettger, Stefan; Bogdanov, Alexey; Boggild, Hans; Bogolyubsky, Mikhail; Boldizsar, Laszlo; Bombara, Marek; Book, Julian; Borel, Herve; Borissov, Alexander; Bose, Suvendu Nath; Bossu, Francesco; Botje, Michiel; Boyer, Bruno Alexandre; Braidot, Ermes; Braun-Munzinger, Peter; Bregant, Marco; Breitner, Timo Gunther; Browning, Tyler Allen; Broz, Michal; Brun, Rene; Bruna, Elena; Bruno, Giuseppe Eugenio; Budnikov, Dmitry; Buesching, Henner; Bufalino, Stefania; Bugaiev, Kyrylo; Busch, Oliver; Buthelezi, Edith Zinhle; Caffarri, Davide; Cai, Xu; Caines, Helen Louise; Calvo Villar, Ernesto; Camerini, Paolo; Canoa Roman, Veronica; Cara Romeo, Giovanni; Carena, Francesco; Carena, Wisla; Carminati, Federico; Casanova Diaz, Amaya Ofelia; Castillo Castellanos, Javier Ernesto; Casula, Ester Anna Rita; Catanescu, Vasile; Cavicchioli, Costanza; Ceballos Sanchez, Cesar; Cepila, Jan; Cerello, Piergiorgio; Chang, Beomsu; Chapeland, Sylvain; Charvet, Jean-Luc Fernand; Chattopadhyay, Sukalyan; Chattopadhyay, Subhasis; Chawla, Isha; Cherney, Michael Gerard; Cheshkov, Cvetan; Cheynis, Brigitte; Chiavassa, Emilio; Chibante Barroso, Vasco Miguel; 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de Rooij, Raoul Stefan; Delagrange, Hugues; Deloff, Andrzej; Demanov, Vyacheslav; Denes, Ervin; Deppman, Airton; Di Bari, Domenico; Di Giglio, Carmelo; Di Liberto, Sergio; Di Mauro, Antonio; Di Nezza, Pasquale; Diaz Corchero, Miguel Angel; Dietel, Thomas; Divia, Roberto; Djuvsland, Oeystein; Dobrin, Alexandru Florin; Dobrowolski, Tadeusz Antoni; Dominguez, Isabel; Donigus, Benjamin; Dordic, Olja; Driga, Olga; Dubey, Anand Kumar; Ducroux, Laurent; Dupieux, Pascal; Dutta Majumdar, AK; Dutta Majumdar, Mihir Ranjan; Elia, Domenico; Emschermann, David Philip; Engel, Heiko; Erdal, Hege Austrheim; Espagnon, Bruno; Estienne, Magali Danielle; Esumi, Shinichi; Evans, David; Eyyubova, Gyulnara; Fabris, Daniela; Faivre, Julien; Falchieri, Davide; Fantoni, Alessandra; Fasel, Markus; Fedunov, Anatoly; Fehlker, Dominik; Feldkamp, Linus; Felea, Daniel; Fenton-Olsen, Bo; Feofilov, Grigory; Fernandez Tellez, Arturo; Ferretti, Alessandro; Ferretti, Roberta; Figiel, Jan; Figueredo, Marcel; Filchagin, Sergey; 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Lee, Sung Chul; Lefevre, Frederic; Lehnert, Joerg Walter; Leistam, Lars; Lemmon, Roy Crawford; Lenhardt, Matthieu Laurent; Lenti, Vito; Leon Monzon, Ildefonso; Leon Vargas, Hermes; Leoncino, Marco; Levai, Peter; Lien, Jorgen; Lietava, Roman; Lindal, Svein; Lindenstruth, Volker; Lippmann, Christian; Lisa, Michael Annan; Liu, Lijiao; Loenne, Per-Ivar; Loggins, Vera; Loginov, Vitaly; Lohn, Stefan Bernhard; Lohner, Daniel; Loizides, Constantinos; Loo, Kai Krister; Lopez, Xavier Bernard; Lopez Torres, Ernesto; Lovhoiden, Gunnar; Lu, Xianguo; Luettig, Philipp; Lunardon, Marcello; Luo, Jiebin; Luparello, Grazia; Luquin, Lionel; Luzzi, Cinzia; Ma, Rongrong; Maevskaya, Alla; Mager, Magnus; Mahapatra, Durga Prasad; Maire, Antonin; Mal'Kevich, Dmitry; Malaev, Mikhail; Maldonado Cervantes, Ivonne Alicia; Malinina, Ludmila; Malzacher, Peter; Mamonov, Alexander; Manceau, Loic Henri Antoine; Manko, Vladislav; Manso, Franck; Manzari, Vito; Mao, Yaxian; Marchisone, Massimiliano; Mares, Jiri; Margagliotti, Giacomo Vito; 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Musa, Luciano; Musso, Alfredo; Nandi, Basanta Kumar; Nania, Rosario; Nappi, Eugenio; Nattrass, Christine; Naumov, Nikolay; Navin, Sparsh; Nayak, Tapan Kumar; Nazarenko, Sergey; Nazarov, Gleb; Nedosekin, Alexander; Nicassio, Maria; Niculescu, Mihai; Nielsen, Borge Svane; Niida, Takafumi; Nikolaev, Sergey; Nikolic, Vedran; Nikulin, Sergey; Nikulin, Vladimir; Nilsen, Bjorn Steven; Nilsson, Mads Stormo; Noferini, Francesco; Nomokonov, Petr; Nooren, Gerardus; Novitzky, Norbert; Nyanin, Alexandre; Nyatha, Anitha; Nygaard, Casper; Nystrand, Joakim Ingemar; Oeschler, Helmut Oskar; Oh, Saehanseul; Oh, Sun Kun; Oleniacz, Janusz; Oppedisano, Chiara; Ortona, Giacomo; Oskarsson, Anders Nils Erik; Otwinowski, Jacek Tomasz; Oyama, Ken; Pachmayer, Yvonne Chiara; Pachr, Milos; Padilla, Fatima; Pagano, Paola; Paic, Guy; Painke, Florian; Pajares, Carlos; Pal, S; Pal, Susanta Kumar; Palaha, Arvinder Singh; Palmeri, Armando; Papikyan, Vardanush; Pappalardo, Giuseppe; Park, Woo Jin; Passfeld, Annika; Patalakha, Dmitri Ivanovich; 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Suire, Christophe Pierre; Sukhorukov, Mikhail; Sultanov, Rishat; Sumbera, Michal; Susa, Tatjana; Szanto de Toledo, Alejandro; Szarka, Imrich; Szczepankiewicz, Adam; Szostak, Artur Krzysztof; Szymanski, Maciej; Takahashi, Jun; Tapia Takaki, Daniel Jesus; Tarazona Martinez, Alfonso; Tauro, Arturo; Tejeda Munoz, Guillermo; Telesca, Adriana; Terrevoli, Cristina; Thader, Jochen Mathias; Thomas, Deepa; Tieulent, Raphael Noel; Timmins, Anthony; Toia, Alberica; Torii, Hisayuki; Tosello, Flavio; Trzaska, Wladyslaw Henryk; Tsuji, Tomoya; Tumkin, Alexandr; Turrisi, Rosario; Tveter, Trine Spedstad; Ulery, Jason Glyndwr; Ullaland, Kjetil; Ulrich, Jochen; Uras, Antonio; Urban, Jozef; Urciuoli, Guido Marie; Usai, Gianluca; Vajzer, Michal; Vala, Martin; Valencia Palomo, Lizardo; Vallero, Sara; van der Kolk, Naomi; van Leeuwen, Marco; Vande Vyvre, Pierre; Vannucci, Luigi; Vargas, Aurora Diozcora; Varma, Raghava; Vasileiou, Maria; Vasiliev, Andrey; Vechernin, Vladimir; Veldhoen, Misha; Venaruzzo, Massimo; Vercellin, Ermanno; Vergara, Sergio; Vernet, Renaud; Verweij, Marta; Vickovic, Linda; Viesti, Giuseppe; Vikhlyantsev, Oleg; Vilakazi, Zabulon; Villalobos Baillie, Orlando; Vinogradov, Alexander; Vinogradov, Leonid; Vinogradov, Yury; Virgili, Tiziano; Viyogi, Yogendra; Vodopianov, Alexander; Voloshin, Kirill; Voloshin, Sergey; Volpe, Giacomo; von Haller, Barthelemy; Vranic, Danilo; Øvrebekk, Gaute; Vrlakova, Janka; Vulpescu, Bogdan; Vyushin, Alexey; Wagner, Boris; Wagner, Vladimir; Wan, Renzhuo; Wang, Dong; Wang, Mengliang; Wang, Yifei; Wang, Yaping; Watanabe, Kengo; Weber, Michael; Wessels, Johannes; Westerhoff, Uwe; Wiechula, Jens; Wikne, Jon; Wilde, Martin Rudolf; Wilk, Alexander; Wilk, Grzegorz Andrzej; Williams, Crispin; Windelband, Bernd Stefan; Xaplanteris Karampatsos, Leonidas; Yaldo, Chris G; Yamaguchi, Yorito; Yang, Hongyan; Yang, Shiming; Yasnopolsky, Stanislav; Yi, JunGyu; Yin, Zhongbao; Yoo, In-Kwon; Yoon, Jongik; Yu, Weilin; Yuan, Xianbao; Yushmanov, Igor; Zach, Cenek; Zampolli, Chiara; Zaporozhets, Sergey; Zarochentsev, Andrey; Zavada, Petr; Zaviyalov, Nikolai; Zbroszczyk, Hanna Paulina; Zelnicek, Pierre; Zgura, Sorin Ion; Zhalov, Mikhail; Zhang, Haitao; Zhang, Xiaoming; Zhou, Daicui; Zhou, Fengchu; Zhou, You; Zhu, Jianhui; Zhu, Xiangrong; Zichichi, Antonino; Zimmermann, Alice; Zinovjev, Gennady; Zoccarato, Yannick Denis; Zynovyev, Mykhaylo; Zyzak, Maksym

    2013-07-16

    Identical neutral kaon pair correlations are measured in $\\sqrt{s}$=7 TeV pp collisions in the ALICE experiment. One-dimensional K$^{0}_{s}$-K$^{0}_{s}$ correlation functions in terms of the invariant momentum difference of kaon pairs are formed in two multiplicity and two transverse momentum ranges. The femtoscopic parameters for the radius and correlation strength of the kaon source are extracted. The fit includes quantum statistics and final-state interactions of the $a_0/f_0$ resonance. K$^{0}_{s}$-K$^{0}_{s}$ correlations show an increase in radius for increasing multiplicity and a slight decrease in radius for increasing transverse mass, $m_T$, as seen in pion-pion correlations in the pp system and in heavy-ion collisions. Transverse mass scaling is observed between the K$^{0}_{s}$-K$^{0}_{s}$ and pion-pion radii. Also, the first observation is made of the decay of the $f_{2}'$(1525) meson into the K$^{0}_{s}$-K$^{0}_{s}$ channel in pp collisions.

  17. Býčí skála: prvé datování skalních maleb

    Czech Academy of Sciences Publication Activity Database

    Svoboda, Jiří; Plicht van der, J.; Balák, I.

    2005-01-01

    Roč. 84, - (2005), s. 574-575 ISSN 0042-4544 Institutional research plan: CEZ:AV0Z80010507 Keywords : Býčí skála * rock art * radiocarbon dating Subject RIV: AC - Archeology, Anthropology, Ethnology

  18. Intracellular synthesis of glutamic acid in Bacillus methylotrophicus SK19.001, a glutamate-independent poly(γ-glutamic acid)-producing strain.

    Science.gov (United States)

    Peng, Yingyun; Zhang, Tao; Mu, Wanmeng; Miao, Ming; Jiang, Bo

    2016-01-15

    Bacillus methylotrophicus SK19.001 is a glutamate-independent strain that produces poly(γ-glutamic acid) (γ-PGA), a polymer of D- and L-glutamic acids that possesses applications in food, the environment, agriculture, etc. This study was undertaken to explore the synthetic pathway of intracellular L- and D-glutamic acid in SK19.001 by investigating the effects of tricarboxylic acid cycle intermediates and different amino acids as metabolic precursors on the production of γ-PGA and analyzing the activities of the enzymes involved in the synthesis of L- and D-glutamate. Tricarboxylic acid cycle intermediates and amino acids could participate in the synthesis of γ-PGA via independent pathways in SK19.001. L-Aspartate aminotransferase, L-glutaminase and L-glutamate synthase were the enzymatic sources of L-glutamate. Glutamate racemase was responsible for the formation of D-glutamate for the synthesis of γ-PGA, and the synthetase had stereoselectivity for glutamate substrate. The enzymatic sources of L-glutamate were investigated for the first time in the glutamate-independent γ-PGA-producing strain, and multiple enzymatic sources of L-glutamate were verified in SK19.001, which will benefit efforts to improve production of γ-PGA with metabolic engineering strategies. © 2015 Society of Chemical Industry.

  19. Carcino-embryonic antigen in monitoring the growth of human colon adenocarcinoma tumour cells SK-CO-1 and HT-29 in vitro and in nude mice

    DEFF Research Database (Denmark)

    Sölétormos, G; Fogh, J M; Sehested-Hansen, B

    1997-01-01

    A set of experimental model systems were designed to investigate (a) the inter-relationship between growth of two human cancer cell lines (SK-CO-1, HT-29) and carcino-embryonic antigen (CEA) kinetics; and (b) whether neoplastic growth or CEA concentration is modulated by human growth hormone (hGH...

  20. MIBG causes oxidative stress and up-regulation of anti-oxidant enzymes in the human neuroblastoma cell line SK-N-BE(2c)

    NARCIS (Netherlands)

    Cornelissen, J.; van Kuilenburg, A. B.; Voûte, P. A.; van Gennip, A. H.

    1997-01-01

    We report the effects of meta-iodobenzylguanidine (MIBG), a neuroblastoma-seeking agent, on cell proliferation and several oxidative stress-related parameters in the human neuroblastoma cell line SK-N-BE(2c). MIBG inhibited the proliferation of this cell line in micromolar concentrations.

  1. A Long-Gap Peripheral Nerve Injury Therapy Using Human Skeletal Muscle-Derived Stem Cells (Sk-SCs): An Achievement of Significant Morphological, Numerical and Functional Recovery.

    Science.gov (United States)

    Tamaki, Tetsuro; Hirata, Maki; Nakajima, Nobuyuki; Saito, Kosuke; Hashimoto, Hiroyuki; Soeda, Shuichi; Uchiyama, Yoshiyasu; Watanabe, Masahiko

    2016-01-01

    Losses in vital functions of the somatic motor and sensory nervous system are induced by severe long-gap peripheral nerve transection injury. In such cases, autologous nerve grafts are the gold standard treatment, despite the unavoidable sacrifice of other healthy functions, whereas the prognosis is not always favorable. Here, we use human skeletal muscle-derived stem cells (Sk-SCs) to reconstitute the function after long nerve-gap injury. Muscles samples were obtained from the amputated legs from 9 patients following unforeseen accidents. The Sk-SCs were isolated using conditioned collagenase solution, and sorted as CD34+/45- (Sk-34) and CD34-/45-/29+ (Sk-DN/29+) cells. Cells were separately cultured/expanded under optimal conditions for 2 weeks, then injected into the athymic nude mice sciatic nerve long-gap model (7-mm) bridging an acellular conduit. After 8-12 weeks, active cell engraftment was observed only in the Sk-34 cell transplanted group, showing preferential differentiation into Schwann cells and perineurial/endoneurial cells, as well as formation of the myelin sheath and perineurium/endoneurium surrounding regenerated axons, resulted in 87% of numerical recovery. Differentiation into vascular cell lineage (pericyte and endothelial cells) were also observed. A significant tetanic tension recovery (over 90%) of downstream muscles following electrical stimulation of the sciatic nerve (at upper portion of the gap) was also achieved. In contrast, Sk-DN/29+ cells were completely eliminated during the first 4 weeks, but relatively higher numerical (83% vs. 41% in axon) and functional (80% vs. 60% in tetanus) recovery than control were observed. Noteworthy, significant increase in the formation of vascular networks in the conduit during the early stage (first 2 weeks) of recovery was observed in both groups with the expression of key factors (mRNA and protein levels), suggesting the paracrine effects to angiogenesis. These results suggested that the human Sk

  2. A Long-Gap Peripheral Nerve Injury Therapy Using Human Skeletal Muscle-Derived Stem Cells (Sk-SCs: An Achievement of Significant Morphological, Numerical and Functional Recovery.

    Directory of Open Access Journals (Sweden)

    Tetsuro Tamaki

    Full Text Available Losses in vital functions of the somatic motor and sensory nervous system are induced by severe long-gap peripheral nerve transection injury. In such cases, autologous nerve grafts are the gold standard treatment, despite the unavoidable sacrifice of other healthy functions, whereas the prognosis is not always favorable. Here, we use human skeletal muscle-derived stem cells (Sk-SCs to reconstitute the function after long nerve-gap injury. Muscles samples were obtained from the amputated legs from 9 patients following unforeseen accidents. The Sk-SCs were isolated using conditioned collagenase solution, and sorted as CD34+/45- (Sk-34 and CD34-/45-/29+ (Sk-DN/29+ cells. Cells were separately cultured/expanded under optimal conditions for 2 weeks, then injected into the athymic nude mice sciatic nerve long-gap model (7-mm bridging an acellular conduit. After 8-12 weeks, active cell engraftment was observed only in the Sk-34 cell transplanted group, showing preferential differentiation into Schwann cells and perineurial/endoneurial cells, as well as formation of the myelin sheath and perineurium/endoneurium surrounding regenerated axons, resulted in 87% of numerical recovery. Differentiation into vascular cell lineage (pericyte and endothelial cells were also observed. A significant tetanic tension recovery (over 90% of downstream muscles following electrical stimulation of the sciatic nerve (at upper portion of the gap was also achieved. In contrast, Sk-DN/29+ cells were completely eliminated during the first 4 weeks, but relatively higher numerical (83% vs. 41% in axon and functional (80% vs. 60% in tetanus recovery than control were observed. Noteworthy, significant increase in the formation of vascular networks in the conduit during the early stage (first 2 weeks of recovery was observed in both groups with the expression of key factors (mRNA and protein levels, suggesting the paracrine effects to angiogenesis. These results suggested that the

  3. Intra-annual distribution of Temora longicornis biomass in the Gulf of Gdańsk (the southern Baltic Sea – numerical simulations

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    Lidia Dzierzbicka-Głowacka

    2017-11-01

    Full Text Available A population model of the copepod Temora longicornis coupled with the ecosystem model 3D CEMBS (Coupled Ecosystem Model of the Baltic Sea was used to determine the intra-annual distribution of the species biomass in the Gdańsk Basin (the southern Baltic Sea. The population model for T. longicornis consists of twelve equations for twelve state variables, six for mass and six for abundance, i.e. two state variables for each of the six model stages of the development: eggs (Egg, non-feeding stage (N1, subsequent nauplii stages (N2–N6, two copepodite stages (C1–C3 and C4–C5 and adults (C6. The empirical validation of the population model was based on in situ data collected in 2010 and 2011 in the Gdańsk Deep and the western part of the Gulf of Gdańsk. The highest values of the model biomass occurred in the period of high water temperatures – in June 2010 and July 2011 in the Gulf of Gdańsk (ca 5200 mg wet weight (w.w. m–2 and 6300 mg w.w. m–2, and for almost the whole summer in the Gdańsk Deep (24 500 mg w.w. m–2 and 27 800 mg w.w. m–2. Temora longicornis produced 4 to 5 generations per year in the Gulf of Gdańsk and Gdańsk Deep, respectively. The population model was satisfactorily verified and the calculated results were consistent with the in situ data. Despite some differences between the field and model data, the developed population model of T. longicornis is the first model for this species in the Baltic Sea and, even though it needs further improvement, it can be a useful tool for determining the population dynamics of the species and ecological relationships in the environment.

  4. Therapeutic action of the mitochondria-targeted antioxidant SkQ1 on retinopathy in OXYS rats linked with improvement of VEGF and PEDF gene expression.

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    Anton M Markovets

    Full Text Available UNLABELLED: The incidence of age-related macular degeneration (AMD, the main cause of blindness in older patients in the developed countries, is increasing with the ageing population. At present there is no effective treatment for the prevailing geographic atrophy, dry AMD, whereas antiangiogenic therapies successful used in managing the wet form of AMD. Recently we showed that mitochondria-targeted antioxidant plastoquinonyl-decyl-triphenylphosphonium (SkQ1 is able to prevent the development and moreover caused regression of pre-existing signs of the retinopathy in OXYS rats, an animal model of AMD. Here we examine the effects of SkQ1 on expression of key regulators of angiogenesis vascular endothelial growth factor A (VEGF and its antagonist pigment epithelium-derived factor (PEDF genes in the retina of OXYS rats as evidenced by real-time PCR and an ELISA test for VEGF using Wistar rats as control. Ophthalmoscopic examinations confirmed that SkQ1 supplementation (from 1.5 to 3 months of age, 250 nmol/kg prevented development while eye drops SkQ1 (250 nM, from 9 to 12 months caused some reduction of retinopathy signs in OXYS rats and did not reveal any negative effects on the control Wistar rat's retina. Prevention of premature retinopathy by SkQ1 was connected with an increase of VEGF mRNA and protein in OXYS rat's retina up to the levels corresponding to the Wistar rats, and did not involve changes in PEDF expression. In contrast the treatment with SkQ1 drops caused a decrease of VEGF mRNA and protein levels and an increase in the PEDF mRNA level in the middle-aged OXYS rats, but in Wistar rats the changes of gene expression were the opposite. CONCLUSIONS: The beneficial effects of SkQ1 on retinopathy connected with normalization of expression of VEGF and PEDF in the retina of OXYS rats and depended on age of the animals and the stage of retinopathy.

  5. PS1-12sk IS A PECULIAR SUPERNOVA FROM A He-RICH PROGENITOR SYSTEM IN A BRIGHTEST CLUSTER GALAXY ENVIRONMENT

    International Nuclear Information System (INIS)

    Sanders, N. E.; Soderberg, A. M.; Foley, R. J.; Chornock, R.; Milisavljevic, D.; Margutti, R.; Drout, M. R.; Moe, M.; Berger, E.; Brown, W. R.; Lunnan, R.; Smartt, S. J.; Fraser, M.; Kotak, R.; Magill, L.; Smith, K. W.; Wright, D.; Huang, K.; Urata, Y.; Mulchaey, J. S.

    2013-01-01

    We report on our discovery and observations of the Pan-STARRS1 supernova (SN) PS1-12sk, a transient with properties that indicate atypical star formation in its host galaxy cluster or pose a challenge to popular progenitor system models for this class of explosion. The optical spectra of PS1-12sk classify it as a Type Ibn SN (SN Ibn; cf. SN 2006jc), dominated by intermediate-width (3 × 10 3 km s –1 ) and time variable He I emission. Our multi-wavelength monitoring establishes the rise time dt ∼ 9-23 days and shows an NUV-NIR spectral energy distribution with temperature ∼> 17 × 10 3 K and a peak magnitude of M z = –18.88 ± 0.02 mag. SN Ibn spectroscopic properties are commonly interpreted as the signature of a massive star (17-100 M ☉ ) explosion within an He-enriched circumstellar medium. However, unlike previous SNe Ibn, PS1-12sk is associated with an elliptical brightest cluster galaxy, CGCG 208–042 (z = 0.054) in cluster RXC J0844.9+4258. The expected probability of an event like PS1-12sk in such environments is low given the measured infrequency of core-collapse SNe in red-sequence galaxies compounded by the low volumetric rate of SN Ibn. Furthermore, we find no evidence of star formation at the explosion site to sensitive limits (Σ Hα ∼ –3 M ☉ yr –1 kpc –2 ). We therefore discuss white dwarf binary systems as a possible progenitor channel for SNe Ibn. We conclude that PS1-12sk represents either a fortuitous and statistically unlikely discovery, evidence for a top-heavy initial mass function in galaxy cluster cooling flow filaments, or the first clue suggesting an alternate progenitor channel for SNe Ibn.

  6. Liquefied Natural Gas Storage of Variable Composition / Magazynowanie Skroplonego Gazu Ziemnego O Zmiennym Składzie

    Science.gov (United States)

    Łaciak, Mariusz

    2015-03-01

    Thanks to the increasing diversification of LNG supply sources, being a result of the growing number of LNG liquefaction installations over the World, increase of short-term trade contracts and general trend to globally liberalize gas markets, reception terminals have to cope with the broad range of qualitatively diversified LNG deliveries from various sources. Different LNG deliveries potentially have different density caused by different gas composition. Although the LNG composition depends on LNG source, it mainly consists of methane, ethane, propane, butane and trace nitrogen. When a new supply of LNG is transported to the tank, the LNG composition and temperature in the tank can be different from LNG as delivered. This may lead to the liquid stratification in the tank, and consequently the rollover. As a result, LNG rapidly evaporates and the pressure in the tank increases. More and more restrictive safety regulations require fuller understanding of the formation and evolution of layers. The paper is focused on the analysis of liquid stratification in the tank which may take place when storing LNG, and which process leads to the rapid evaporation of considerable quantities of LNG. The aim was to attempt modeling of the process of liquid stratification in an LNG tank. The paper is closed with the results of modelling. Dzięki rosnącej dywersyfikacji źródeł dostaw LNG, spowodowanej zwiększającą się liczbą instalacji skraplania gazu na całym świecie, wzrostem ilości kontraktów krótkoterminowych w handlu i ogólnej tendencji do globalnej liberalizacja rynków gazu, terminale do odbioru muszą radzić sobie z coraz większą gamą różnych jakościowo dostaw LNG z różnych źródeł. Różne dostawy LNG mają potencjalnie inną gęstość dzięki różnym składom gazu. Chociaż kompozycja LNG zależy od źródła, to przede wszystkim składa się z metanu, etanu, propanu, butanu i w śladowych ilościach z azotu. Gdy nowa dostawa LNG jest

  7. METCAM/MUC18 promoted tumorigenesis of human breast cancer SK-BR-3 cells in a dosage-specific manner.

    Science.gov (United States)

    Huang, Chang-Yu; Wu, Guang-Jer

    2016-04-01

    Overexpression of METCAM/MUC18, an immunoglobulin-like cell-adhesion molecule, promotes tumorigenesis and progression of human breast cancer cells. We also observed an intriguing phenomenon that a high-expressing SK-BR-3 clone manifested a transient tumor suppression effect in vivo. The purpose of this study was to understand if this was caused by clonal variation, METCAM/MUC18-dosage effect, or the number of cells injected. Several G418-resistant clones of SK-BR-3, expressing different levels of METCAM/MUC18, were obtained for testing effects of human METCAM/MUC18 on in vitro motility, invasiveness, and anchorage-independent colony formation (in vitro tumorigenicity) and in vivo tumorigenesis in female Balb/C athymic nude mice. Tumor sections were made for histology and immunohistochemistry analyses, and tumor lysates for Western blot analysis to determine the effects of human METCAM/MUC18 expression on levels of various downstream effectors. METCAM/MUC18 promoted in vitro motility, invasiveness, and in vitro tumorigenicity of SK-BR-3 cells in a dosage-specific manner. Overexpression of METCAM/MUC18 could promote in vivo tumorigenesis of SK-BR-3 cells even when one tenth of the previously used cell number (5 × 10(5)) was injected and in vivo tumorigenesis of SK-BR-3 cells was directly proportional to the dosage of the protein. The previously observed transient tumor suppression effect from the same clone was no longer observed. The downstream effector, such as phospho-AKT/AKT ratio, was elevated in the tumors. Transient suppression observed previously in the clone was caused by injection of a high cell number (2 × 10(6)-5 × 10(6)). METCAM/MUC18 positively promotes tumorigenesis of SK-BR-3 cells by increasing the survival and proliferation pathway. Copyright © 2016. Published by Elsevier B.V.

  8. Confirming a Role for α9nAChRs and SK Potassium Channels in Type II Hair Cells of the Turtle Posterior Crista

    Directory of Open Access Journals (Sweden)

    Xiaorong Xu Parks

    2017-11-01

    Full Text Available In turtle posterior cristae, cholinergic vestibular efferent neurons (VENs synapse on type II hair cells, bouton afferents innervating type II hair cells, and afferent calyces innervating type I hair cells. Electrical stimulation of VENs releases acetylcholine (ACh at these synapses to exert diverse effects on afferent background discharge including rapid inhibition of bouton afferents and excitation of calyx-bearing afferents. Efferent-mediated inhibition is most pronounced in bouton afferents innervating type II hair cells near the torus, but becomes progressively smaller and briefer when moving longitudinally through the crista toward afferents innervating the planum. Sharp-electrode recordings have inferred that efferent-mediated inhibition of bouton afferents requires the sequential activation of alpha9-containing nicotinic ACh receptors (α9*nAChRs and small-conductance, calcium-dependent potassium channels (SK in type II hair cells. Gradations in the strength of efferent-mediated inhibition across the crista likely reflect variations in α9*nAChRs and/or SK activation in type II hair cells from those different regions. However, in turtle cristae, neither inference has been confirmed with direct recordings from type II hair cells. To address these gaps, we performed whole-cell, patch-clamp recordings from type II hair cells within a split-epithelial preparation of the turtle posterior crista. Here, we can easily visualize and record hair cells while maintaining their native location within the neuroepithelium. Consistent with α9*nAChR/SK activation, ACh-sensitive currents in type II hair cells were inward at hyperpolarizing potentials but reversed near −90 mV to produce outward currents that typically peaked around −20 mV. ACh-sensitive currents were largest in torus hair cells but absent from hair cells near the planum. In current clamp recordings under zero-current conditions, ACh robustly hyperpolarized type II hair cells. ACh

  9. A synergistic antiproliferation effect of curcumin and docosahexaenoic acid in SK-BR-3 breast cancer cells: unique signaling not explained by the effects of either compound alone

    International Nuclear Information System (INIS)

    Altenburg, Jeffrey D; Bieberich, Andrew A; Terry, Colin; Harvey, Kevin A; VanHorn, Justin F; Xu, Zhidong; Jo Davisson, V; Siddiqui, Rafat A

    2011-01-01

    Breast cancer is a collection of diseases in which molecular phenotypes can act as both indicators and mediators of therapeutic strategy. Therefore, candidate therapeutics must be assessed in the context of multiple cell lines with known molecular phenotypes. Docosahexaenoic acid (DHA) and curcumin (CCM) are dietary compounds known to antagonize breast cancer cell proliferation. We report that these compounds in combination exert a variable antiproliferative effect across multiple breast cell lines, which is synergistic in SK-BR-3 cells and triggers cell signaling events not predicted by the activity of either compound alone. Dose response curves for CCM and DHA were generated for five breast cell lines. Effects of the DHA+ CCM combination on cell proliferation were evaluated using varying concentrations, at a fixed ratio, of CCM and DHA based on their individual ED 50 . Detection of synergy was performed using nonlinear regression of a sigmoid dose response model and Combination Index approaches. Cell molecular network responses were investigated through whole genome microarray analysis of transcript level changes. Gene expression results were validated by RT-PCR, and western blot analysis was performed for potential signaling mediators. Cellular curcumin uptake, with and without DHA, was analyzed via flow cytometry and HPLC. CCM+DHA had an antiproliferative effect in SK-BR-3, MDA-MB-231, MDA-MB-361, MCF7 and MCF10AT cells. The effect was synergistic for SK-BR-3 (ER - PR - Her2 + ) relative to the two compounds individually. A whole genome microarray approach was used to investigate changes in gene expression for the synergistic effects of CCM+DHA in SK-BR-3 cells lines. CCM+DHA triggered transcript-level responses, in disease-relevant functional categories, that were largely non-overlapping with changes caused by CCM or DHA individually. Genes involved in cell cycle arrest, apoptosis, inhibition of metastasis, and cell adhesion were upregulated, whereas genes

  10. Agrin-LRP4-MuSK signaling as a therapeutic target for myasthenia gravis and other neuromuscular disorders.

    Science.gov (United States)

    Ohno, Kinji; Ohkawara, Bisei; Ito, Mikako

    2017-10-01

    Signal transduction at the neuromuscular junction (NMJ) is compromised in a diverse array of diseases including myasthenia gravis, Lambert-Eaton myasthenic syndrome, Isaacs' syndrome, congenital myasthenic syndromes, Fukuyama-type congenital muscular dystrophy, amyotrophic lateral sclerosis, and sarcopenia. Except for sarcopenia, all are orphan diseases. In addition, the NMJ signal transduction is impaired by tetanus, botulinum, curare, α-bungarotoxin, conotoxins, organophosphate, sarin, VX, and soman to name a few. Areas covered: This review covers the agrin-LRP4-MuSK signaling pathway, which drives clustering of acetylcholine receptors (AChRs) and ensures efficient signal transduction at the NMJ. We also address diseases caused by autoantibodies against the NMJ molecules and by germline mutations in genes encoding the NMJ molecules. Expert opinion: Representative small compounds to treat the defective NMJ signal transduction are cholinesterase inhibitors, which exert their effects by increasing the amount of acetylcholine at the synaptic space. Another possible therapeutic strategy to enhance the NMJ signal transduction is to increase the number of AChRs, but no currently available drug has this functionality.

  11. Antiproliferative effects of Plumbago rosea and its purified constituent plumbagin on SK-MEL 28 melanoma cell lines.

    Science.gov (United States)

    Anuf, Alexander Ronaldo; Ramachandran, Rajesh; Krishnasamy, Rajaram; Gandhi, P S Sudhakar; Periyasamy, Sureshkumar

    2014-10-01

    Plumbago rosea is used in traditional systems of medicine for the preparation of formulations used for treating inflammations, cough, bronchitis, and gastrointestinal disorders, and also in conjunction with cancer chemotherapy. In the present study, the cytotoxic and anti-proliferative effects of plumbagin, and the ethanolic root extract of P. rosea (ETPR) was evaluated on SK-MEL 28 melanoma cell lines and human lymphocytes. MTT and apoptotic assays were used for the evaluation of cytotoxic and anti-proliferative effects, respectively. In addition, the effect of Plumbagin and ETPR in down regulation of BCL-2 expression is investigated using RT-PCR analysis. Both plumbagin and ETPR dose-dependently decreased the cell viability more potently in melanoma cell lines. P. rosea extract demonstrated significant synergy in inhibiting BCL-2 expression than plumbagin. Moreover plumbagin showed more toxicity in human lymphocytes. Plumbagin has anti-cancer potential, but the side effects limits its use; yet plumbagin, in combination with other ingredients in Plumbago rosea extract, displays significant synergy leading to a stronger anticancer effect with significantly less toxicity.

  12. Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel Regulation

    Directory of Open Access Journals (Sweden)

    Anett Illing

    2013-01-01

    Full Text Available Pluripotent stem cells present an extraordinary powerful tool to investigate embryonic development in humans. Essentially, they provide a unique platform for dissecting the distinct mechanisms underlying pluripotency and subsequent lineage commitment. Modest information currently exists about the expression and the role of ion channels during human embryogenesis, organ development, and cell fate determination. Of note, small and intermediate conductance, calcium-activated potassium channels have been reported to modify stem cell behaviour and differentiation. These channels are broadly expressed throughout human tissues and are involved in various cellular processes, such as the after-hyperpolarization in excitable cells, and also in differentiation processes. To this end, human induced pluripotent stem cells (hiPSCs generated from plucked human hair keratinocytes have been exploited in vitro to recapitulate endoderm formation and, concomitantly, used to map the expression of the SK channel (SKCa subtypes over time. Thus, we report the successful generation of definitive endoderm from hiPSCs of ectodermal origin using a highly reproducible and robust differentiation system. Furthermore, we provide the first evidence that SKCas subtypes are dynamically regulated in the transition from a pluripotent stem cell to a more lineage restricted, endodermal progeny.

  13. Immobilization of α-Amylase from Anoxybacillus sp. SK3-4 on ReliZyme and Immobead Supports

    Directory of Open Access Journals (Sweden)

    Ummirul Mukminin Kahar

    2016-09-01

    Full Text Available α-Amylase from Anoxybacillus sp. SK3-4 (ASKA is a thermostable enzyme that produces a high level of maltose from starches. A truncated ASKA (TASKA variant with improved expression and purification efficiency was characterized in an earlier study. In this work, TASKA was purified and immobilized through covalent attachment on three epoxide (ReliZyme EP403/M, Immobead IB-150P, and Immobead IB-150A and an amino-epoxide (ReliZyme HFA403/M activated supports. Several parameters affecting immobilization were analyzed, including the pH, temperature, and quantity (mg of enzyme added per gram of support. The influence of the carrier surface properties, pore sizes, and lengths of spacer arms (functional groups on biocatalyst performances were studied. Free and immobilized TASKAs were stable at pH 6.0–9.0 and active at pH 8.0. The enzyme showed optimal activity and considerable stability at 60 °C. Immobilized TASKA retained 50% of its initial activity after 5–12 cycles of reuse. Upon degradation of starches and amylose, only immobilized TASKA on ReliZyme HFA403/M has comparable hydrolytic ability with the free enzyme. To the best of our knowledge, this is the first report of an immobilization study of an α-amylase from Anoxybacillus spp. and the first report of α-amylase immobilization using ReliZyme and Immobeads as supports.

  14. First report on intersex in invasive round goby Neogobius melanostomus from the Baltic Sea (Gulf of Gdańsk, Poland

    Directory of Open Access Journals (Sweden)

    Tatiana Guellard

    2015-01-01

    Full Text Available This study is the first to report the presence of intersex in invasive round goby Neogobius melanostomus inhabiting the Baltic Sea. The discovery was made in the area of two harbours of the Gulf of Gdańsk (Poland. Macro- and microscopic male gonad analysis revealed the presence of female gametes in testes (testis-ova of single specimens. In addition, a female-like urogenital papilla was observed in one of the intersex fish. These findings might be due to the exposure to estrogenic endocrine disrupting compounds (EDCs however the occurrence of single intersex individuals as a baseline level in investigated population is also possible. In the future, more comprehensive research in the other areas of the Gulf of Gdańsk needs to be carried out in order to determine the extent and to better understand the cause of the observed phenomenon.

  15. Sjuksköterskor och skötares upplevelser av möten i psykiatrisk öppenvård : En intervjustudie

    OpenAIRE

    Bäckman, Jenny; Nyman, Nyman

    2016-01-01

    Syftet med studien var att undersöka sjuksköterskors och skötares upplevelser av möten med patienter i psykiatrisk öppenvård.   Bakgrund – I psykiatrisk öppenvård möter sjuksköterskor och skötare många olika människor med individuella behov. Sedan tidigare forskning har det framkommit att patienters känsla av trygghet uppkommer genom faktorer som personalens inställning och empati. Relationen mellan personal och patient är viktig för patientens känsla av att bli tagen på allvar samt att bli b...

  16. Ocena przydatności kryteriów Williamsa w rozpoznawaniu atopowego zapalenia skóry w praktyce lekarza pediatry

    Directory of Open Access Journals (Sweden)

    Zbigniew Samochocki

    2009-09-01

    Full Text Available O trudnościach w rozpoznawaniu AZS u dzieci świadczy stosowanie wielu różnych metod diagnostycznych. Jedną z nich są kryteria zaproponowane przez Williamsa iwsp. Celem pracy było określenie przydatności kryteriów Williamsa iwsp. wrozpoznawaniu AZS u dzieci poprzez ocenę porównawczą z kryteriami Hanifina iRajki. Badaniami objęto 166 pacjentów Poradni Dermatologicznej (67 chłopców i 99 dziewczynek w wieku od 4 do 15 lat (średnio 10,3 roku, którzy niezależnie od zmian skórnych zgłaszali świąd skóry. Poddano ich badaniu klinicznemu i procedurom zawartym w kryteriach Hanifina i Rajki. Dokonano analizy statystycznej wyników. U 108 dzieci rozpoznano AZS, a u 58 – świerzb, łojotokowe zapalenie skóry, zapalenie skóry z podrażnienia, łuszczycę, trądzik młodzieńczy oraz pokrzywkę. U wszystkich chorych naAZS wykazano suchość skóry. Zajęcie typowych okolic podawane wwywiadzie, początek zmian skórnych <2. roku życia, czynne zmiany w przegubach i osobnicze obciążenie atopią obserwowano odpowiednio u 96,3% (104/108, 71,3% (77/108, 67,6% (73/108 i 64,3% (70/108 pacjentów. Wszystkie te cechy występowały statystycznie istotnie częściej (p<0,001 wporównaniu z grupą dzieci, u których nie rozpoznano wyprysku atopowego. Stosując kryteria Williamsa i wsp., u 3/108 chorych wykazano fałszywie ujemne wyniki, a u 2/58 – fałszywie dodatnie. Czułość i swoistość tej metody badawczej wynosiła odpowiednio 97,2% i 96,6%. Wnioski: 1. Kryteria diagnostyczne Williamsa i wsp. są bardzo pomocne w praktyce lekarza pediatry w rozpoznawaniu AZS u dzieci powyżej 3. roku życia. 2. Suchość skóry i potwierdzenie wwywiadzie obecności zmian wypryskowych o typowej lokalizacji są najbardziej przydatne w rozpoznawaniu AZS u dzieci. 3. W przypadkach wątpliwych rozszerzenie granicy wieku wystąpienia pierwszych zmian skórnych z 2 do 5 lat zwiększa wykrywalność choroby.

  17. Neuroprotective effects of corn silk maysin via inhibition of H2O2-induced apoptotic cell death in SK-N-MC cells.

    Science.gov (United States)

    Choi, Doo Jin; Kim, Sun-Lim; Choi, Ji Won; Park, Yong Il

    2014-07-25

    Neuroprotective effects of maysin, which is a flavone glycoside that was isolated from the corn silk (CS, Zea mays L.) of a Korean hybrid corn Kwangpyeongok, against oxidative stress (H2O2)-induced apoptotic cell death of human neuroblastoma SK-N-MC cells were investigated. Maysin cytotoxicity was determined by measuring cell viability using MTT and lactate dehydrogenase (LDH) assays. Intracellular reactive oxygen species (ROS) were measured using a 2,7-dichlorofluorescein diacetate (DCF-DA) assay. Apoptotic cell death was monitored by annexin V-FITC/PI double staining and by a TUNEL assay. Antioxidant enzyme mRNA levels were determined by real-time PCR. The cleavage of poly (ADP-ribose) polymerase (PARP) was measured by western blotting. Maysin pretreatment reduced the cytotoxic effect of H2O2 on SK-N-MC cells, as shown by the increase in cell viability and by reduced LDH release. Maysin pretreatment also dose-dependently reduced the intracellular ROS level and inhibited PARP cleavage. In addition, DNA damage and H2O2-induced apoptotic cell death were significantly attenuated by maysin pretreatment. Moreover, maysin pretreatment (5-50 μg/ml) for 2h significantly and dose-dependently increased the mRNA levels of antioxidant enzymes (CAT, GPx-1, SOD-1, SOD-2 and HO-1) in H2O2 (200 μM)-insulted cells. These results suggest that CS maysin has neuroprotective effects against oxidative stress (H2O2)-induced apoptotic death of human brain SK-N-MC cells through its antioxidative action. This report is the first regarding neuroprotective health benefits of corn silk maysin by its anti-apoptotic action and by triggering the expression of intracellular antioxidant enzyme systems in SK-N-MC cells. Copyright © 2014 Elsevier Inc. All rights reserved.

  18. Analýza a návrh online marketingovej stratégie spoločnosti AQT.sk

    OpenAIRE

    Múdry, Daniel

    2017-01-01

    The diploma thesis deals with the online marketing strategy and online activities of the company AQT.sk. The aim of the thesis is to analyze this strategy, individual tools and activities that the company uses and to propose further steps to improve the performance and efficiency of online marketing as a component of the communication mix. Theoretical part briefly describes the development of online marketing and at the same time presents in detail individual online marketing tools and their ...

  19. Antioxidant mechanism of mitochondria-targeted plastoquinone SkQ1 is suppressed in aglycemic HepG2 cells dependent on oxidative phosphorylation

    Czech Academy of Sciences Publication Activity Database

    Ježek, J.; Engstová, Hana; Ježek, Petr

    2017-01-01

    Roč. 1858, č. 9 (2017), s. 750-762 ISSN 0005-2728 R&D Projects: GA ČR(CZ) GA17-01813S Institutional support: RVO:67985823 Keywords : mitochondria-targeted antioxidant SkQ1 * mitochondrial Complex I superoxide formation * mitochondrial Complex III superoxide formation * HepG2 cell s * NAD(P)H fluorescence lifetime imaging microscopy Subject RIV: ED - Physiology OBOR OECD: Cell biology Impact factor: 4.932, year: 2016

  20. Urbánno-rurálně rozdiely voličského správania v Česku a na Slovensku

    Czech Academy of Sciences Publication Activity Database

    Kostelecký, Tomáš; Krivý, V.

    2015-01-01

    Roč. 47, č. 4 (2015), s. 390-413 ISSN 0049-1225 R&D Projects: GA ČR(CZ) GAP408/12/1442 Institutional support: RVO:68378025 Keywords : voting behaviour * aggregated data * Czech and Slovak society Subject RIV: AO - Sociology , Demography Impact factor: 0.275, year: 2015 https://www.sav.sk/journals/uploads/08190954Krivy%20-%20Kostelecky%20OK.pdf

  1. RNA sequence analyses of r-Moj-DM treated cells: TXNIP is required to induce apoptosis of SK-Mel-28.

    Science.gov (United States)

    McBride, Terri D; Andrew, U; Ly, Nicko; Soto, Julio G

    2016-10-01

    RNA sequencing of untreated and r-Moj-DM treated SK-Mel-28 cells was performed after 6 h, to begin unraveling the apoptotic pathway induced by r-Moj-DM. Bioinformatic analyses of RNA sequencing data yielded 40 genes that were differentially expressed. Nine genes were upregulated and 31 were downregulated. qRT-PCR was used to validate differential expression of 13 genes with known survival or apoptotic-inducing activities. Expression of BNiP3, IGFBP3, PTPSF, Prune 2, TGF-ß, and TXNIP were compared from cells treated with r-Moj-DN (a strong apoptotic inducer) or r-Moj-DA (a non-apoptotic inducer) for 1 h, 2 h, 4 h, and 6 h after treatment. Our results demonstrate that significant differences in expression are only detected after 4 h of treatment. In addition, expression of TXNIP (an apoptotic inducer) remains elevated at 4 h and 6 h only in r-Moj-DN treated cells. Based on the consistency of elevated TXNIP expression, we further studied TXNIP as a novel target of disintegrin activation. Confocal microscopy of anti-TXNIP stained SK-Mel-28 cells suggests nuclear localization of TXNIP after r-Moj-DM treatment. A stable TXNIP knockdown SK-Mel-28 cell line was produced to test TXNIP' role in the apoptotic induction by r-Moj-DM. High cell viability (74.3% ±9.1) was obtained after r-Moj-DM treatment of TXNIP knocked down SK-Mel-28 cells, compared to 34% ±0.187 for untransduced cells. These results suggest that TXNIP is required early in the apoptotic-inducing pathway resulting from r-Moj-DM binding to the αv integrin subunit. Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. ‘Close to the city centre, close to the university’. Are there symptoms of studentification in Gdańsk, Poland?

    OpenAIRE

    Grabkowska Maja; Frankowski Jan

    2016-01-01

    Students are increasingly recognised as important actors of urban change in contemporary cities. The article presents an analysis of incipient studentification processes in Gdańsk. Its general aim is to reveal patterns of students’ presence and activity which translate into spatial and socio-economic transformations of a post-socialist city at the level of neighbourhoods. The study consists of a survey on students’ residential behaviour, complemented by field and desk research. According to t...

  3. Felicjan Sławoj-Składkowski (1885-1962): The only known doctor-soldier-politician with an outdoor-wooden-toilet named after him.

    Science.gov (United States)

    Ohry, Avi

    2017-05-01

    Felicjan Sławoj-Składkowski was a Polish physician, General and politician who served as Polish Minister of Internal Affairs and was the last Prime Minister of Poland before the Second World War. The lack of basic sanitation in many of Poland's villages caused him to issue a decree that every household in Poland must have a latrine in working order. Wooden sheds were built in the backyards, subsequently named 'sławojkis'.

  4. Andrographolide promotes vincristine-induced SK-NEP-1 tumor cell death via PI3K-AKT-p53 signaling pathway

    Science.gov (United States)

    Zhang, Mingsheng; Xue, Enda; Shao, Wei

    2016-01-01

    Background Nephroblastoma (Wilms’ tumor [WT]) is the most common malignant renal cancer in children. Although the outcome of WT has significantly improved as a result of the combination of surgery, chemotherapy, and radiotherapy; in some cases WT results in severe complications. Thus, novel strategies that would decrease treatment burden are required. The aim of the current study was to investigate the synergistic antitumor effect of andrographolide (AND) in combination with vincristine (VCR) on WT cells. Methods Cell Counting Kit-8 assay was used to investigate the synergistic antiproliferation effect of AND and/or VCR on SK-NEP-1 cells in vitro. Meanwhile, SK-NEP-1 xenografts were used to detect the antitumor effect in vivo. Apoptosis and autophagy were then detected by Annexin V, monodansylcadaverine staining. Finally, the underlying signaling transduction was determined with Western blotting. Results The combination of AND with VCR significantly suppressed SK-NEP-1 cell proliferation in vitro and inhibited xenograft tumor growth in vivo, compared with AND or VCR treatment alone. In addition, the synergistic antitumor effect of AND on the cells was due to an increased apoptosis, not autophagy. Moreover, PI3K-AKT-p53 signaling pathway was involved in the process of combination treatment, which was confirmed when a selective AKT activator was applied. Conclusion The combination of AND with VCR has a strong synergistic antitumor effect on WT via PI3K-AKT-p53 signaling pathway, thereby representing a potential treatment for WT in the near future. PMID:27729773

  5. The effect of VMP35 supplement ingredients encapsulated in a novel Phospholipid Prodosome SK713 SLP nutrient delivery technology on properties of live human blood.

    Directory of Open Access Journals (Sweden)

    Kenneth Blum

    2015-09-01

    Full Text Available Background: In North America digestive malfunction in terms of disintegration, dissolution, and absorption of food and nutrients, is a widespread malady. Malabsorption is also an exacerbating factor in most chronic degenerative diseases that might benefit from dietary supplementation. The purpose of this experiment was to determine, as shown by changes in properties of live blood, whether, a novel soy-lecithin-phospholipid-nutrient encapsulation technology could promote rapid bioavailability and bioactivity of a VMP35 nutraceutical formulation encapsulated within its clustoidal multilamellar Soy Lecithin Phospholipid (SLP liquid SK713 SLP structures. Method: Changes in peripheral blood smears from 38 subjects were measured utilizing peripheral live blood cell imaging (LBCI with phase contrast microscopy. Results: Compared to baseline and control, consistently and reproducibly, the SK713 SLP technology effected positive changes in the blood as demonstrated by observable morphological, hematological and rheological changes five minutes from intake and sustained for at least 30 minutes post intake. Conclusions: These results showed that the SK713 SLP system makes an important contribution by increasing the potential benefits of dietary supplementation to those patients with compromised digestive processes. We encourage additional research on this novel delivery system believing that it has potential impact on future therapy.

  6. Distribution and fate of polycyclic aromatic hydrocarbons (PAHs in recent sediments from the Gulf of Gdańsk (SE Baltic

    Directory of Open Access Journals (Sweden)

    Ludwik Lubecki

    2010-12-01

    Full Text Available Polycyclic aromatic hydrocarbons (PAHs were determined in recent (0-10 cm sediments from the Gulf of Gdańsk during 2003-07 andcorrelated with environmental parameters. Located in the south-eastern part of the Baltic and receiving the waters of the River Wisła(Vistula, this area of negligible tides and strong anthropogenic stress, highly eutrophic as a consequence, is an exceptionalmodel basin for studying the fate of hydrophobic organic contaminants introduced to the sea. Environmental conditions determine thedistribution and composition patterns of parent PAHs in Gulf of Gdańsk sediments. PAHs were associated mainly with fine particlesediments, rich in organic carbon, with hypoxia/anoxia near the bottom. The highest PAH contents were found in the Gdańsk Deep(ca 110 m, where the mean concentration of Σ12PAHs was ~3600 ng g-1, and no distinct temporal trend was observed.Lighter PAHs were found to be depleted in deeper regions. It was estimated that the Wisła discharges ~50% of the total PAH load deposited in recent Gulf sediments.

  7. Loss of function mutation in LARP7, chaperone of 7SK ncRNA, causes a syndrome of facial dysmorphism, intellectual disability, and primordial dwarfism.

    Science.gov (United States)

    Alazami, Anas M; Al-Owain, Mohammad; Alzahrani, Fatema; Shuaib, Taghreed; Al-Shamrani, Hussain; Al-Falki, Yahya H; Al-Qahtani, Saleh M; Alsheddi, Tarfa; Colak, Dilek; Alkuraya, Fowzan S

    2012-10-01

    Primordial dwarfism (PD) is a clinically and genetically heterogeneous condition. Various molecular mechanisms are known to underlie the disease including impaired mitotic mechanics, abnormal IGF2 expression, perturbed DNA damage response, defective spliceosomal machinery, and abnormal replication licensing. Here, we describe a syndromic form of PD associated with severe intellectual disability and distinct facial features in a large multiplex Saudi family. Analysis reveals a novel underlying mechanism for PD involving depletion of 7SK, an abundant cellular noncoding RNA (ncRNA), due to mutation of its chaperone LARP7. We show that 7SK levels are tightly linked to LARP7 expression across cell lines, and that this chaperone is ubiquitously expressed in the mouse embryo. The 7SK is known to influence the expression of a wide array of genes through its inhibitory effect on the positive transcription elongation factor b (P-TEFb) as well as its competing role in HMGA1-mediated transcriptional regulation. This study documents a critical role played by ncRNA in human development and adds to the growing list of molecular mechanisms that, when perturbed, converge on the PD phenotype. © 2012 Wiley Periodicals, Inc.

  8. Nekonvenční metody zpracování nožířských materiálů

    OpenAIRE

    Bražina, Jakub

    2017-01-01

    Tato práce se zaobírá problematikou nekonvenčních nožířských materiálu a jejich následnou aplikaci pro výrobu a zpracování loveckého nože. V rešeršní části je čtenáři přiblížena nožířská problematika, kde jsou uvedeny základní konstrukční charakteristiky a potřebné vlastnosti nože. V další části práce jsou popsány různé kovové i nekovové materiály, jejich vlastnosti, případné zpracování a výroba. Dále se práce zabývá problematikou povrchové ochrany těchto nožířských materiálu. Práce se také z...

  9. The Apoptotic Effect of Ursolic Acid on SK-Hep-1 Cells is Regulated by the PI3K/Akt, p38 and JNK MAPK Signaling Pathways

    Directory of Open Access Journals (Sweden)

    Wan-Ling Chuang

    2016-04-01

    Full Text Available Ursolic acid (UA is a pentacyclic triterpene acid that is present in a wide variety of medicinal herbs and edible plants. This study investigated the effect of UA on apoptosis and proliferation of hepatocellular carcinoma SK-Hep-1 cells. After treatment of SK-Hep-1 cells with different concentrations of UA, we observed that cell viability was reduced in a dose- and time-dependent manner. Furthermore, there was a dose-dependent increase in the percentage of cells in the sub-G1 and G2/M phases, with cells treated with 60 μM showing the highest percentages of cells in those phases. UA-induced chromatin condensation of nuclei was observed by using DAPI staining. The western blot results revealed that exposure to UA was associated with decreased expression of the anti-apoptotic proteins Mcl-1, Bcl-xL, Bcl-2, and TCTP and increased expression of apoptosis-related proteins TNF-α, Fas, FADD, Bax, cleaved caspase-3, caspase-8, caspase-9, and PARP. Immunocytochemistry staining showed that treatment with UA resulted in increased expression of caspase-3. Moreover, exposure to UA resulted in the inhibition of the PI3K/Akt and p38 MAPK signaling pathways. These findings suggest that UA inhibits the proliferation of SK-Hep-1 cells and induces apoptosis.

  10. Chronic exposure of Sk-1 hairless mice to narrow-band ultraviolet A (320-355 nm)

    International Nuclear Information System (INIS)

    Menter, J.M.; Sayre, R.M.; Etemadi, A.A.; Agin, P.P.; Wills, I.

    1996-01-01

    Several recent investigations collectively suggest that the role of ultraviolet A (UVA) in chronic actinic skin damage may be greater than originally thought. In the present work, the output of a xenon-arc solar-simulator passed through a Bausch and Lomb monochromator in conjunction with a 2-mm Schott WG-320 filter produced narrow-band UVA centered at 338 nm, half-band width 24 nm, I 0 =3.4±0.3 mW/cm 2 . We chronically irradiated 10 SK-1 albino hairless mice 5 times per week for 18 weeks, starting with 1.25 J/cm 2 , for 33 irradiation days, sequentially followed by 1.50 J/cm 2 (34 days), 1.8 J/cm 2 (10 days), 2.0 J/cm 2 (22 days) to afford a total UVA dose of 154.3 J/cm 2 over 99 irradiation days. Erythema was noted clinically by day 6, which persisted throughout the irradiation. During the irradiation period, some scaling, consistent with mild epidermal hyperplasia was noted during irradiation days 37-56. This response later regressed despite continued chronic irradiation. Hematoxylin and eosin examination immediately after the final irradiation revealed a mild inflammatory response, with some dermal restructuring. At the end of the experiment, no significant signs of epidermal hyperplasia or (pre)malignant lesions were seen, although some stratum corneum thickening was noted. Marked dermal collagen damage and moderate elastosis was also evident. We believe that the observed differences in results reported in previous studies are in large part due to differences in light sources and irradiation protocols. (au)

  11. Evaluation of radiation-induced genotoxicity on human melanoma cells (SK-MEL-37) by flow cytometry

    International Nuclear Information System (INIS)

    Bonfim, Leticia; Carvalho, Luma Ramirez de; Vieira, Daniel Perez

    2017-01-01

    Micronucleus assay is a test used to evaluate genotoxic damage in cells, which can be caused by various factors, like ionizing radiation. Interactions between radiation energies and DNA can cause breakage, leading to use chromosomal mutations or loss of genetic material, important events that could be induced in solid tumors to mitigate its expansion within human body. Melanoma has been described as a tumor with increased radio resistance. This work evaluated micronuclei percentages (%MN) in human melanoma cells (SK-MEL-37), irradiated by gamma radiation, with doses between 0 and 16Gy. Cell suspensions were irradiated in PBS by a "6"0Co source in doses between 0 and 16Gy, and incubated by 48h. Then cell membranes were lysed in the presence of SYTOX Green and EMA dyes, preserving nuclear membranes. Using this method, EMA-stained nuclei could be discriminated as those derived from dead cells, and SYTOX nuclei and micronuclei could be quantified. Micronuclei percentages were found to be proportional to dose, (R2 = 0.997). Only the highest dose (16Gy) could induce statistically significant increase of MN (p<0.0001), although cultures irradiated by 4, 8 and 16Gy showed significant increase of dead cell fractions. Calculation of the nuclei-to-beads ratio showed that 8 and 16Gy could reduce melanoma cell proliferation. Results showed that although cell death and loss of proliferative capacity could be observed on cultures irradiated at lower doses, genotoxic damage could be induced only on a higher dose. Resistance to radiation-induced genotoxicity could explain a relatively high radio resistance of melanoma tumors. (author)

  12. Evaluation of radiation-induced genotoxicity on human melanoma cells (SK-MEL-37) by flow cytometry

    Energy Technology Data Exchange (ETDEWEB)

    Bonfim, Leticia; Carvalho, Luma Ramirez de; Vieira, Daniel Perez, E-mail: leticia.bonfim@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2017-11-01

    Micronucleus assay is a test used to evaluate genotoxic damage in cells, which can be caused by various factors, like ionizing radiation. Interactions between radiation energies and DNA can cause breakage, leading to use chromosomal mutations or loss of genetic material, important events that could be induced in solid tumors to mitigate its expansion within human body. Melanoma has been described as a tumor with increased radio resistance. This work evaluated micronuclei percentages (%MN) in human melanoma cells (SK-MEL-37), irradiated by gamma radiation, with doses between 0 and 16Gy. Cell suspensions were irradiated in PBS by a {sup 60}Co source in doses between 0 and 16Gy, and incubated by 48h. Then cell membranes were lysed in the presence of SYTOX Green and EMA dyes, preserving nuclear membranes. Using this method, EMA-stained nuclei could be discriminated as those derived from dead cells, and SYTOX nuclei and micronuclei could be quantified. Micronuclei percentages were found to be proportional to dose, (R2 = 0.997). Only the highest dose (16Gy) could induce statistically significant increase of MN (p<0.0001), although cultures irradiated by 4, 8 and 16Gy showed significant increase of dead cell fractions. Calculation of the nuclei-to-beads ratio showed that 8 and 16Gy could reduce melanoma cell proliferation. Results showed that although cell death and loss of proliferative capacity could be observed on cultures irradiated at lower doses, genotoxic damage could be induced only on a higher dose. Resistance to radiation-induced genotoxicity could explain a relatively high radio resistance of melanoma tumors. (author)

  13. PS1-12sk IS A PECULIAR SUPERNOVA FROM A He-RICH PROGENITOR SYSTEM IN A BRIGHTEST CLUSTER GALAXY ENVIRONMENT

    Energy Technology Data Exchange (ETDEWEB)

    Sanders, N. E.; Soderberg, A. M.; Foley, R. J.; Chornock, R.; Milisavljevic, D.; Margutti, R.; Drout, M. R.; Moe, M.; Berger, E.; Brown, W. R.; Lunnan, R. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Smartt, S. J.; Fraser, M.; Kotak, R.; Magill, L.; Smith, K. W.; Wright, D. [Astrophysics Research Centre, School of Maths and Physics, Queens University, Belfast BT7 1NN (United Kingdom); Huang, K. [Academia Sinica Institute of Astronomy and Astrophysics, Taipei 106, Taiwan (China); Urata, Y. [Institute of Astronomy, National Central University, Chung-Li 32054, Taiwan (China); Mulchaey, J. S., E-mail: nsanders@cfa.harvard.edu [Carnegie Observatories, 813 Santa Barbara Street, Pasadena, CA 91101 (United States); and others

    2013-05-20

    We report on our discovery and observations of the Pan-STARRS1 supernova (SN) PS1-12sk, a transient with properties that indicate atypical star formation in its host galaxy cluster or pose a challenge to popular progenitor system models for this class of explosion. The optical spectra of PS1-12sk classify it as a Type Ibn SN (SN Ibn; cf. SN 2006jc), dominated by intermediate-width (3 Multiplication-Sign 10{sup 3} km s{sup -1}) and time variable He I emission. Our multi-wavelength monitoring establishes the rise time dt {approx} 9-23 days and shows an NUV-NIR spectral energy distribution with temperature {approx}> 17 Multiplication-Sign 10{sup 3} K and a peak magnitude of M{sub z} = -18.88 {+-} 0.02 mag. SN Ibn spectroscopic properties are commonly interpreted as the signature of a massive star (17-100 M{sub Sun }) explosion within an He-enriched circumstellar medium. However, unlike previous SNe Ibn, PS1-12sk is associated with an elliptical brightest cluster galaxy, CGCG 208-042 (z = 0.054) in cluster RXC J0844.9+4258. The expected probability of an event like PS1-12sk in such environments is low given the measured infrequency of core-collapse SNe in red-sequence galaxies compounded by the low volumetric rate of SN Ibn. Furthermore, we find no evidence of star formation at the explosion site to sensitive limits ({Sigma}{sub H{alpha}} {approx}< 2 Multiplication-Sign 10{sup -3} M{sub Sun} yr{sup -1} kpc{sup -2}). We therefore discuss white dwarf binary systems as a possible progenitor channel for SNe Ibn. We conclude that PS1-12sk represents either a fortuitous and statistically unlikely discovery, evidence for a top-heavy initial mass function in galaxy cluster cooling flow filaments, or the first clue suggesting an alternate progenitor channel for SNe Ibn.

  14. The Chinese Cretaceous Continental Scientific Drilling Project in the Songliao Basin, NE China: Organic-rich source rock evaluation with geophysical logs from Borehole SK-2

    Science.gov (United States)

    Zhang, X.; Zou, C.

    2017-12-01

    The Cretaceous strata have been recognized as an important target of oil or gas exploration in the Songliao Basin, northeast China. The second borehole (SK-2) of the Chinese Cretaceous Continental Scientific Drilling Project in the Songliao Basin (CCSD-SK) is the first one to drill through the Cretaceous continental strata in the frame of ICDP. It was designed not only to solve multiple scientific problems (including the Cretaceous paleoenvironment and paleoclimate, as well as deep resources exploration of the Songliao Basin), but also to expect to achieve new breakthroughs in oil and gas exploration. Based on the project, various geophysical log data (including gamma, sonic, resistivity, density etc.) and core samples have been collected from Borehole SK-2. We do research on organic-rich source rocks estimation using various geophysical log data. Firstly, we comprehensively analyzed organic-rich source rocks' geophysical log response characteristics. Then, source rock's identification methods were constructed to identify organic-rich source rocks with geophysical logs. The main identification methods include cross-plot, multiple overlap and Decision Tree method. Finally, the technique and the CARBOLOG method were applied to evaluate total organic carbon (TOC) content from geophysical logs which provide continuous vertical profile estimations (Passey, 1990; Carpentier et al., 1991). The results show that source rocks are widely distributed in Borehole SK-2, over a large depth strata (985 5700m), including Nenjiang, Qingshankou, Denglouku, Yingcheng, Shahezi Formations. The organic-rich source rocks with higher TOC content occur in the Qingshankou (1647 1650m), Denglouku (2534 2887m) and Shahezi (3367 5697m) Formations. The highest TOC content in these formations can reach 10.31%, 6.58%, 12.79% respectively. The bed thickness of organic-rich source rocks in the these formations are totally up to 7.88m, 74.34m, 276.60m respectively. These organic-rich rocks in the

  15. Pro-inflammatory cytokines derived from West Nile virus (WNV-infected SK-N-SH cells mediate neuroinflammatory markers and neuronal death

    Directory of Open Access Journals (Sweden)

    Nerurkar Vivek R

    2010-10-01

    Full Text Available Abstract Background WNV-associated encephalitis (WNVE is characterized by increased production of pro-inflammatory mediators, glial cells activation and eventual loss of neurons. WNV infection of neurons is rapidly progressive and destructive whereas infection of non-neuronal brain cells is limited. However, the role of neurons and pathological consequences of pro-inflammatory cytokines released as a result of WNV infection is unclear. Therefore, the objective of this study was to examine the role of key cytokines secreted by WNV-infected neurons in mediating neuroinflammatory markers and neuronal death. Methods A transformed human neuroblastoma cell line, SK-N-SH, was infected with WNV at multiplicity of infection (MOI-1 and -5, and WNV replication kinetics and expression profile of key pro-inflammatory cytokines were analyzed by plaque assay, qRT-PCR, and ELISA. Cell death was measured in SK-N-SH cell line in the presence and absence of neutralizing antibodies against key pro-inflammatory cytokines using cell viability assay, TUNEL and flow cytometry. Further, naïve primary astrocytes were treated with UV-inactivated supernatant from mock- and WNV-infected SK-N-SH cell line and the activation of astrocytes was measured using flow cytometry and ELISA. Results WNV-infected SK-N-SH cells induced the expression of IL-1β, -6, -8, and TNF-α in a dose- and time-dependent manner, which coincided with increase in virus-induced cell death. Treatment of cells with anti-IL-1β or -TNF-α resulted in significant reduction of the neurotoxic effects of WNV. Furthermore treatment of naïve astrocytes with UV-inactivated supernatant from WNV-infected SK-N-SH cell line increased expression of glial fibrillary acidic protein and key inflammatory cytokines. Conclusion Our results for the first time suggest that neurons are one of the potential sources of pro-inflammatory cytokines in WNV-infected brain and these neuron-derived cytokines contribute to WNV

  16. Osmotic stress confers enhanced cell integrity to hydrostatic pressure but impairs growth in Alcanivorax borkumensis SK2

    Directory of Open Access Journals (Sweden)

    Alberto eScoma

    2016-05-01

    Full Text Available Alcanivorax is a hydrocarbonoclastic genus dominating oil spills worldwide. While its presence has been detected in oil-polluted seawaters, marine sediment and salt marshes under ambient pressure, its presence in deep-sea contaminated environments is negligible. Recent laboratory evidences highlighted the piezosensitive nature of some Alcanivorax species, whose growth yields are highly impacted by mild hydrostatic pressures (HPs. In the present study, osmotic stress was used as a tool to increase HP resistance in the type strain A. borkumensis SK2. Control cultures grown under standard conditions of salinity and osmotic pressure with respect to seawater (35.6 ppt or 1136 mOsm kg-1, respectively were compared with cultures subjected to hypo- and hyperosmosis (330 and 1720 mOsm kg-1, or 18 and 62 ppt in salinity, equivalent to brackish and brine waters, respectively, under atmospheric or increased HP (0.1 and 10MPa. Osmotic stress had a remarkably positive impact on cell metabolic activity in terms of CO2 production (thus, oil bioremediation and O2 respiration under hyperosmosis, as acclimation to high salinity enhanced cell activity under 10MPa by a factor of 10. Both osmotic shocks significantly enhanced cell protection by reducing membrane damage under HP, with cell integrities close to 100% under hyposmosis. The latter was likely due to intracellular water-reclamation as no trace of the piezolyte ectoine was found, contrary to hyperosmosis. Notably, ectoine production was equivalent at 0.1MPa in hyperosmosis-acclimated cells and at 10MPa under isosmotic conditions, supporting the hypothesis that ectoine synthesis may be primarily triggered by HP rather than osmotic stress. While stimulating cell metabolism and enhancing cell integrity, osmotic stress had always a negative impact on culture growth and performance. No net growth was observed during 4-day incubation tests, and CO2:O2 ratios and pH values indicated that culture performance in

  17. Andrographolide promotes vincristine-induced SK-NEP-1 tumor cell death via PI3K-AKT-p53 signaling pathway

    Directory of Open Access Journals (Sweden)

    Zhang M

    2016-09-01

    Full Text Available Mingsheng Zhang, Enda Xue, Wei Shao Department of Pediatric Surgery, Liaocheng People’s Hospital, Liaocheng, Shandong Province, People’s Republic of China Background: Nephroblastoma (Wilms’ tumor [WT] is the most common malignant renal cancer in children. Although the outcome of WT has significantly improved as a result of the combination of surgery, chemotherapy, and radiotherapy; in some cases WT results in severe complications. Thus, novel strategies that would decrease treatment burden are required. The aim of the current study was to investigate the synergistic antitumor effect of andrographolide (AND in combination with vincristine (VCR on WT cells.Methods: Cell Counting Kit-8 assay was used to investigate the synergistic antiproliferation effect of AND and/or VCR on SK-NEP-1 cells in vitro. Meanwhile, SK-NEP-1 xenografts were used to detect the antitumor effect in vivo. Apoptosis and autophagy were then detected by Annexin V, monodansylcadaverine staining. Finally, the underlying signaling transduction was determined with Western blotting.Results: The combination of AND with VCR significantly suppressed SK-NEP-1 cell proliferation in vitro and inhibited xenograft tumor growth in vivo, compared with AND or VCR treatment alone. In addition, the synergistic antitumor effect of AND on the cells was due to an increased apoptosis, not autophagy. Moreover, PI3K-AKT-p53 signaling pathway was involved in the process of combination treatment, which was confirmed when a selective AKT activator was applied.Conclusion: The combination of AND with VCR has a strong synergistic antitumor effect on WT via PI3K-AKT-p53 signaling pathway, thereby representing a potential treatment for WT in the near future. Keywords: andrographolide, vincristine, p53, drug combination

  18. Seed oil composition of Acacia nilotica (L. Delile from Iran / Skład oleju z nasion Acacia nilotica (L. Delile rosnącej w Iranie

    Directory of Open Access Journals (Sweden)

    Abbasian Karim

    2016-03-01

    Full Text Available Wstęp: Acacia nilotica (L. Delile należy do rodziny Fabaceae, podrodziny Mimosoideae; otrzymuje się z niej gumę arabską. W południowym Iranie są spożywane pieczone młode strąki i nasiona tej rośliny Cel: Badano skład oleju z dojrzałych nasion A. nilotica zebranych z naturalnych stanowisk na południu Iranu w celu określenia jego przydatności do spożycia przez ludzi i zwierzęta. Metody: Wyekstrahowany olej analizowano metodą chromatografii gazowej sprzężonej ze spektrometrią mas (GC/MS. Wyniki: Zawartość oleju w jadalnych nasionach wynosiła 3.4% (v/w świeżej masy. Olej zawierał rzadko spotykany fitosterol, sześć kwasów tłuszczowych, dziewięć węgolwodorów i jeden diterpenoid; związki te stanowiły łącznie około 83.5% oleju. Głównymi składnikami oleju były: fitosterol, 26-ethylcholesta-5,25(Z-dien-3.β-ol (20.8% oraz nasycone i nienasycone kwasy tłuszczowe. Zawartość pozostałych składników nie przekroczyła 5%. Wniosek: Olej z nasion omawianego gatunku może być nowym naturalnym środkiem odżywczym dla ludzi.

  19. German register for detection of late sequelae after radiotherapy for children and adolescents (RiSK): present status and first results

    International Nuclear Information System (INIS)

    Boelling, T.; Schuck, A.; Willich, N.; Pape, H.; Ruebe, C.; Meyer, F.M.; Martini, C.; Timmermann, B.; Asadpour, B.; Kortmann, R.D.; Beck, J.D.; Langer, T.; Paulides, M.

    2007-01-01

    Undoubtedly, radiotherapy is an important treatment strategy for many malignancies in paediatric oncology. However, sufficient data about late side effects do not exist. There is a lack of information about radiation dose-effect relationships in view of late side effects in childhood and adolescence. Late effects after radiotherapy in childhood and adolescence have mainly been characterized retrospectively with small patient numbers. Many of these analyses are limited due to little information about organ dose levels and older radiation techniques in some cases. Therefore the German Group of Paediatric Radiation Oncology (APRO) established the ''Register for the evaluation of late side effects after radiation in childhood and adolescence (RiSK)''. Aim of this prospective multicentric register study is to evaluate irradiation dose effect relationships of organs and part of organs with respect to late effects. The feasibility of RiSK has already been shown and first results have recently been published. The characterization of late effects after cancer therapy in childhood is of rising interest. In Germany, several study groups like the ''Late Effects Surveillance System'' (LESS) or the working group ''Quality of life'' examine different aspects of late effects. In the United States of America, the ''Childhood Cancer Survivor Study'' has been established to characterize retrospectively the health status of 5-year-survivors of childhood cancer. In these studies, more than 12,000 patients were evaluated by questionnaires regarding their health status. For radiotherapy, this study is not able to give detailed information about late side effects due to rare data about radiation doses and organ dose levels. To our knowledge, ''RiSK'' is the only multicentric study that evaluates radiation associated side effects prospectively with detailed information about organ dose levels. (orig.)

  20. Specific pesticide-dependent increases in α-synuclein levels in human neuroblastoma (SH-SY5Y) and melanoma (SK-MEL-2) cell lines.

    Science.gov (United States)

    Chorfa, Areski; Bétemps, Dominique; Morignat, Eric; Lazizzera, Corinne; Hogeveen, Kevin; Andrieu, Thibault; Baron, Thierry

    2013-06-01

    Epidemiological studies indicate a role of genetic and environmental factors in Parkinson's disease involving alterations of the neuronal α-synuclein (α-syn) protein. In particular, a relationship between Parkinson's disease and occupational exposure to pesticides has been repeatedly suggested. Our objective was to precisely assess changes in α-syn levels in human neuroblastoma (SH-SY5Y) and melanoma (SK-MEL-2) cell lines following acute exposure to pesticides (rotenone, paraquat, maneb, and glyphosate) using Western blot and flow cytometry. These human cell lines express α-syn endogenously, and overexpression of α-syn (wild type or mutated A53T) can be obtained following recombinant adenoviral transduction. We found that endogenous α-syn levels in the SH-SY5Y neuroblastoma cell line were markedly increased by paraquat, and to a lesser extent by rotenone and maneb, but not by glyphosate. Rotenone also clearly increased endogenous α-syn levels in the SK-MEL-2 melanoma cell line. In the SH-SY5Y cell line, similar differences were observed in the α-syn adenovirus-transduced cells, with a higher increase of the A53T mutated protein. Paraquat markedly increased α-syn in the SK-MEL-2 adenovirus-transduced cell line, similarly for the wild-type or A53T proteins. The observed differences in the propensities of pesticides to increase α-syn levels are in agreement with numerous reports that indicate a potential role of exposure to certain pesticides in the development of Parkinson's disease. Our data support the hypothesis that pesticides can trigger some molecular events involved in this disease and also in malignant melanoma that consistently shows a significant but still unexplained association with Parkinson's disease.

  1. A numerical investigation of phytoplankton and Pseudocalanus elongatus dynamics in the spring bloom time in the Gdańsk Gulf

    Science.gov (United States)

    Dzierzbicka-Głowacka, Lidia

    2005-01-01

    A nutrient-phytoplankton-zooplankton-detritus (1D-NPZD) `phytoplankton {Phyt} and Pseudocalanus elongatus {Zoop} dynamics in the spring bloom time in the Gdańsk Gulf. The 1D-NPZD model consists of three coupled, partial second-order differential equations of the diffusion type for phytoplankton {Phyt}, zooplankton {Zoop}, nutrients {Nutr} and one ordinary first-order differential equation for benthic detritus pool {Detr}, together with initial and boundary conditions. In this model, the {Zoop} is presented by only one species of copepod ( P. elongatus) and {Zoop} is composed of six cohorts of copepods with weights ( Wi) and numbers ( Zi); where Zoop= limit∑i=16W iZ i. The calculations were made for 90 days (March, April, May) for two stations at Gdańsk Gulf with a vertical space step of 0.5m and a time step of 900 s. The flow field and water temperature used as the inputs in the biological model 1D-NPZD were reproduced by the prognostic numerical simulation technique using hydrographic climatological data. The results of the numerical investigations described here were compared with the mean observed values of surface chlorophyll- a and depth integrated P. elongatus biomass for 10 years, 1980-1990. The slight differences between the calculated and mean observed values of surface chlorophyll- a and zooplankton biomass are ca. 10-60 mg C m -3 and ca. 5-23 mg C m -2, respectively, depending on the location of the hydrographic station. The 1D-NPZD model with a high-resolution zooplankton module for P. elongatus can be used to describe the temporal patterns for phytoplankton biomass and P. elongatus in the centre of the Gdańsk Gulf.

  2. Polska w oczach guwernera Skórzewskich, czyli zapiski księdza Pocharda z lat 1792–1833 w zbiorach Biblioteki Uniwersyteckiej w Poznaniu

    Directory of Open Access Journals (Sweden)

    Renata Wilgosiewicz-Skutecka

    2011-01-01

    Full Text Available W zbiorach rękopisów przechowywanych w Bibliotece Uniwersyteckiej w Poznaniu znajdują się trzy tomy wspomnień Claude’a Antoine’a Pocharda – francuskiego księdza, żyjącego w czasach rewolucji francuskiej, należącego do prêtres réfractaires i w roku 1792 zmuszonego do opuszczenia ojczyzny. W latach 1792–1796 pozostawał on na emigracji w Szwajcarii, później przyjął posadę guwernera synów Józefa Skórzewskiego, starosty gnieźnieńskiego, i Heleny z Lipskich Skórzewskiej. Zapiski księdza Pocharda prowadzone w latach 1792–1833 dotyczą obu tych okresów. Ich lektura daje możliwość śledzenia losów duchownego w trudnych czasach rewolucyjnych, a także zagłębienia się w relacje naocznego świadka wydarzeń z życia rodzinnego Skórzewskich, wydarzeń ukazanych w szerszym kontekście historycznym – polskim i europejskim. Artykuł jest wprowadzeniem do badania tych rękopisów jako wielowątkowego materiału mogącego zainteresować zarówno literaturoznawców, jak i historyków, pozwalającego weryfikować wiedzę i stawiać hipotezy dotyczące dziejów jednego z najznamienitszych rodów wielkopolskich.

  3. German register for detection of late sequelae after radiotherapy for children and adolescents (RiSK): present status and first results

    Energy Technology Data Exchange (ETDEWEB)

    Boelling, T.; Schuck, A.; Willich, N. [Dept. of Radiotherapy of the Univ. Hospital Muenster (Germany); Pape, H. [Dept. of Radiotherapy of the Univ. Hospital Duesseldorf (Germany); Ruebe, C. [Dept. of Radiotherapy of the Univ. Saarland, Homburg/Saar (Germany); Meyer, F.M. [Dept. of Radiotherapy of the Clinic of Augsburg (Germany); Martini, C. [Dept. of Radiotherapy of the Univ. Hospitals Freiburg (Germany); Timmermann, B. [Paul Scherrer Inst., Villigen (Switzerland); Asadpour, B. [Dept. of Radiotherapy of the Univ. Hospital Aachen (Germany); Kortmann, R.D. [Dept. of Radiotherapy of the Univ. Hospital Leipzig (Germany); Beck, J.D.; Langer, T.; Paulides, M. [LESS Center, Univ. Children' s Hospital Erlangen (Germany)

    2007-12-15

    Undoubtedly, radiotherapy is an important treatment strategy for many malignancies in paediatric oncology. However, sufficient data about late side effects do not exist. There is a lack of information about radiation dose-effect relationships in view of late side effects in childhood and adolescence. Late effects after radiotherapy in childhood and adolescence have mainly been characterized retrospectively with small patient numbers. Many of these analyses are limited due to little information about organ dose levels and older radiation techniques in some cases. Therefore the German Group of Paediatric Radiation Oncology (APRO) established the 'Register for the evaluation of late side effects after radiation in childhood and adolescence (RiSK)'. Aim of this prospective multicentric register study is to evaluate irradiation dose effect relationships of organs and part of organs with respect to late effects. The feasibility of RiSK has already been shown and first results have recently been published. The characterization of late effects after cancer therapy in childhood is of rising interest. In Germany, several study groups like the 'Late Effects Surveillance System' (LESS) or the working group 'Quality of life' examine different aspects of late effects. In the United States of America, the 'Childhood Cancer Survivor Study' has been established to characterize retrospectively the health status of 5-year-survivors of childhood cancer. In these studies, more than 12,000 patients were evaluated by questionnaires regarding their health status. For radiotherapy, this study is not able to give detailed information about late side effects due to rare data about radiation doses and organ dose levels. To our knowledge, 'RiSK' is the only multicentric study that evaluates radiation associated side effects prospectively with detailed information about organ dose levels. (orig.)

  4. 10Be exposure age chronology of the last glaciation of the Roháčská Valley in the Western Tatra Mountains, central Europe

    Science.gov (United States)

    Engel, Zbyněk; Mentlík, Pavel; Braucher, Régis; Křížek, Marek; Pluháčková, Markéta; Arnold, Maurice; Aumaître, Georges; Bourlès, Didier; Keddadouche, Karim; Aster Team; Arnold, Maurice; Aumaître, Georges; Bourlès, Didier; Keddadouche, Karim

    2017-09-01

    10Be exposure ages from moraines and bedrock sites in the Roháčská Valley provide chronology of the last glaciation in the largest valley of the Western Tatra Mts., the Western Carpathians. The minimum apparent exposure age of 19.4 ± 2.1 ka obtained for the oldest sampled boulder and the mean age of 18.0 ± 0.8 ka calculated for the terminal moraine indicate that the oldest preserved moraine was probably deposited at the time of the global Last Glacial Maximum (LGM). The age of this moraine coincides with the termination of the maximum glacier expansion in other central European ranges, including the adjacent High Tatra Mts. and the Alps. The equilibrium line altitude (ELA) of the LGM glacier in the Roháčská Valley, estimated at 1400-1410 m a.s.l., was 50-80 m lower than in the eastern part of the range, indicating a positive ELA gradient from west to east among the north-facing glaciers in the Tatra Mts. Lateglacial glacier expansion occurred no later than 13.4 ± 0.5 ka and 11.9 ± 0.5 ka, as indicated by the mean exposure ages calculated for re-advance moraines. This timing is consistent with the exposure age chronology of the last Lateglacial re-advance in the High Tatra Mts., Alps and lower mountain ranges in central Europe. The ELA in the Roháčská Valley estimated at 1690-1770 m a.s.l. in this period was located 130-300 m lower than in the north-facing valleys in the High Tatra Mts. 10Be exposure ages obtained for a rock glacier constrains the timing of this landform stabilization in the Salatínska Valley and provides the first chronological evidence for the Lateglacial activity of rock glaciers in the Carpathians.

  5. The presence of c-erbB-2 gene product-related protein in culture medium conditioned by breast cancer cell line SK-BR-3

    International Nuclear Information System (INIS)

    Alper, O.; Yamaguchi, K.; Hitomi, J.; Honda, S.; Matsushima, T.; Abe, K.

    1990-01-01

    The Mr 185,000 glycoprotein encoded by human c-erbB-2/neu/HER2 gene, termed c-erbB-2 gene product, shows a close structural similarity with epidermal growth factor receptor and is now regarded to be a growth factor receptor for an as yet unidentified ligand. Abundant c-erbB-2 mRNA was demonstrated by Northern blot studies in the human breast cancer cell line SK-BR-3. Cellular radiolabeling experiments followed by immunoprecipitation with three different anti-c-erbB-2 gene product antibodies, recognizing extracellular domain, kinase domain, and carboxyl-terminal portion, respectively, demonstrated the production of a large amount of c-erbB-2 gene product which had the capacity to be phosphorylated. Immunization of mice with concentrated culture medium conditioned by SK-BR-3 cells always generated antibodies against c-erbB-2 gene product, demonstrating that this culture medium contained substance(s) immunologically indistinguishable from c-erbB-2 gene product. This observation was supported by the successful development of a monoclonal antibody against c-erbB-2 gene product, GFD-OA-p185-1, by immunizing mice with this culture medium. The biochemical nature of the substance(s) present in the culture medium was further characterized. When the culture medium conditioned by [35S]cysteine-labeled SK-BR-3 cells was immunoprecipitated by three different anti-c-erbB-2 gene product antibodies, only the antibody recognizing extracellular domain precipitated the [35S]-labeled protein with a molecular weight of 110,000, namely p110. The newly developed monoclonal antibody also immunoprecipitated this protein

  6. BMP9 inhibits proliferation and metastasis of HER2-positive SK-BR-3 breast cancer cells through ERK1/2 and PI3K/AKT pathways.

    Science.gov (United States)

    Ren, Wei; Liu, Yuehong; Wan, Shaoheng; Fei, Chang; Wang, Wei; Chen, Yingying; Zhang, Zhihui; Wang, Ting; Wang, Jinshu; Zhou, Lan; Weng, Yaguang; He, Tongchuan; Zhang, Yan

    2014-01-01

    Bone morphogenetic protein 9 (BMP9), a member of TGF-β superfamily, is reported to inhibit the growth and migration of prostate cancer, osteosarcoma and triple-negative MDA-MB-231 breast cancer cells. However, little is known about the effect of on the biological behaviors of HER2-positive SK-BR-3 breast cancer cells and the underlying mechanisms. This study aimed to investigate the effects of BMP9 on the proliferation and metastasis of SK-BR-3 cells with BMP9 over-expression or BMP9 down-regulated expression. Results indicated that exogenously expressed BMP9 inhibited the proliferation and metastasis of SK-BR-3 cells while decreased endogenous BMP9 expression in SK-BR-3 cells promoted the proliferation and migration of breast cancer cells in vitro and in vivo. In SK-BR-3 cells with BMP9 over-expression, the phosphorylation of HER2, ERK1/2 and AKT was markedly suppressed and the HER2 expression decreased at both mRNA and protein levels, while opposite results were observed in SK-BR-3 cells with BMP9 knock down. When the phosphorylation of ERK1/2 and PI3K/AKT was inhibited by PD98059 and LY294002, respectively, the decreased proliferation and invasion induced by BMP9 knock down were eliminated. These findings suggest that BMP9 can inhibit the proliferation and metastasis of SK-BR-3 cells via inactivating ERK1/2 and PI3K/AKT signaling pathways. Thus, BMP9 may serve as a useful agent in the treatment of HER-2 positive breast cancer.

  7. SkQ1 Ophthalmic Solution for Dry Eye Treatment: Results of a Phase 2 Safety and Efficacy Clinical Study in the Environment and During Challenge in the Controlled Adverse Environment Model.

    Science.gov (United States)

    Petrov, Anton; Perekhvatova, Natalia; Skulachev, Maxim; Stein, Linda; Ousler, George

    2016-01-01

    This Phase 2 clinical trial assessed the efficacy and safety of the novel antioxidative, renewable compound SkQ1 for topical treatment of dry eye signs and symptoms. In a single-center, randomized, double-masked, placebo-controlled, 29-day study, 91 subjects with mild to moderate dry eye instilled the study drug twice daily and recorded dry eye symptoms daily. Subjects were randomized 1:1:1 into one of three ophthalmic solution treatment groups: SkQ1 1.55 µg/mL, SkQ1 0.155 µg/mL, or 0.0 µg/mL (placebo). Subjects were exposed to a controlled adverse environment chamber at 3 of the 4 study visits (Day -7, Day 1, and Day 29). Investigator assessments occurred at all study visits. SkQ1 was safe and efficacious in treating dry eye signs and symptoms. Statistically significant improvements with SkQ1 compared to placebo occurred for the dry eye signs of corneal fluorescein staining and lissamine green staining in the central region and lid margin redness, and for the dry eye symptoms of ocular discomfort, dryness, and grittiness. In addition, SkQ1 demonstrated greater efficacy compared to placebo, although the differences were not statistically significant, for corneal fluorescein staining in other regions and/or time points (total staining score, central region, corneal sum score, and temporal region), lissamine green staining for the central and nasal regions, and blink rate scores. This Phase 2 study indicated that SkQ1 is safe and efficacious for the treatment of dry eye signs and symptoms and supported previous study results. Clinicaltrials.gov identifier: NCT02121301. Miotech S.A.

  8. Etteheited räsivad pidu / Henri Reeder, Ly Rääsk, Heli Jürgenson ... [jt.] ; intervjueerinud Anneli Aasmäe

    Index Scriptorium Estoniae

    2009-01-01

    Küsimustele tänavusele üldlaulu- ja üldtantsupeole minekust ja peo repertuaari raskusest vastavad Aruküla noortekoori laulja Henri Reeder, Äksi segakoori dirigent Ly Rääsk, üldlaulupeo segakooride liigijuht Heli Jürgenson, Laulu- ja Tantsupeo SA muusikatoimetaja Ave Sopp, Laulu- ja Tantsupeo SA tantsutoimetaja Kadri Tiis , Lüganuse segakoori dirigent Priit-Andres Pärtna ja kolme tantsurühma juhendaja Vändrast Kädi Pärnoja

  9. Finanční analýza podniku Drůbežářský závod Klatovy a.s.

    OpenAIRE

    Kohoutová, Kateřina

    2010-01-01

    This bachelor thesis deals with the financial analysis of Drůbežářský závod Klatovy a.s. during the period 2005-2009. The goal of the thesis is to evaluate the economic situation of the corporation and find out its competitive strength. A part of the project is the methodology which qualifies concepts and indicators and creates bases for the practical part. In the practical part of the project horizontal and vertical analysis of balance sheet and profit and loss statement with comments are pe...

  10. The Skælskør structure in eastern Denmark – wrench-related anticline or primary Late Cretaceous sea-floor topography?

    DEFF Research Database (Denmark)

    Surlyk, Finn; Boldreel, Lars Ole; Lykke-Andersen, Holger

    2010-01-01

    seismic studies of the Chalk Group in Øresund and Kattegat have shown that similar highs actually represent topographic highs on the Late Cretaceous – Danian seafloor formed by strong contourparallel bottom currents. Reflection seismic data collected over the Skælskør structure in order to test...... the Ringkøbing Fyn High into the Danish Basin. The elevated position is maintained due to reduced subsidence as compared with the Danish Basin north of the high. The hypothesis of wrench tectonics as origin can be refuted. The seismic data show that the upper part of the Chalk Group is characterised by irregular...

  11. Hydraulic conditions of water flow in seminatural fish pass, A case study of the Skórka barrage on the Głomia river

    Directory of Open Access Journals (Sweden)

    Mateusz Hämmerling

    2017-06-01

    Full Text Available The article presents results of a field study of the fish pass located within the Skórka barrage on the Głomia river. The aim of the study was to identify water flow conditions in particular chambers of the fish pass. On the basis of results of the field study, the hydraulic conditions of water flow through the fish pass were determined and referred to the optimum performance parameters of the construction. The results obtained make a basis for discussion of possible problems related to construction and operation of the fish passes resembling „close to nature” structures.

  12. Finančná analýza podniku Choceňská mlékárna, s.r.o.

    OpenAIRE

    Zigo, Michal

    2011-01-01

    The bachelor thesis assesses a financial position of the company Choceňská mlékárna, s.r.o. with the help of financial analysis tools such as horizontal and vertical analyses, parallel and pyramidal system of financial ratios, creditworthy and bankruptcy models, and economic value added in the horizon of five years. Theoretical-methodological part describes the financial analysis as a tool for an assessment of the financial position of the company and it further defines the evaluation apparat...

  13. Składniki tworzyw sztucznych zaburzające funkcje układu nerwowego

    Directory of Open Access Journals (Sweden)

    Konrad Andrzej Szychowski

    2013-05-01

    Full Text Available Rozwój przemysłu chemicznego spowodował opracowywanie nowych związków chemicznych niewystępujących w środowisku naturalnym. Substancje te spełniają różnorodne funkcje, takie jak obniżenie palności, zwiększenie plastyczności czy poprawienie rozpuszczalności innych substancji. Wiele związków wchodzących w skład tworzyw sztucznych, kosmetyków nowej generacji, aparatury medycznej, opakowań spożywczych oraz innych produktów codziennego użytku, z łatwością uwalniają się do środowiska naturalnego. Liczne badania wykazały, że substancje, takie jak: ftalany, BPA, TBBPA oraz PCB charakteryzują się znaczną lipofilnością dzięki czemu w łatwy sposób wnikają do żywych komórek oraz akumulują się w tkankach i narządach. Niesie to za sobą zagrożenie dla zdrowia ludzi i zwierząt. Wymienione związki, ze względu na swoją budowę chemiczną, są zdolne do naśladowania endogennych hormonów np. estradiolu, zaburzając hormonalną homeostazę organizmu. Co więcej substancje te z łatwością pokonują barierę łożyskową oraz barierę krew-mózg i dlatego, jak wykazały liczne badania, zaburzają prawidłowe działanie układu nerwowego już od najwcześniejszych chwil życia. Udowodniono, że związki te wpływają na proces neurogenezy oraz transmisję synaptyczną, a w konsekwencji zaburzają proces kształtowania się płci mózgu, a także procesy uczenia, pamięci, zachowania. Ponadto ich cytotoksyczne i proapoptotyczne działanie może być przyczyną powstawania chorób neurodegeneracyjnych. Praca przedstawia aktualny stan wiedzy na temat oddziaływania ftalanów, BPA, TBBPA, oraz PCB na układ nerwowy.

  14. Grasshoppers (Orthoptera: Caelifera and crickets (Orthoptera: Ensifera from slopes of Macošská stráň and Vilémovická stráň (Moravský kras Protected landscape area, Czech Republic

    Directory of Open Access Journals (Sweden)

    Jana Niedobová

    2011-01-01

    Full Text Available In 2008 we found 21 species of grasshoppers and crickets on Macošská stráň slope and 18 species on Vilémovická stráň slope. Both slopes are located in the northern part of the Moravský kras Protected landscape area and have xerothermic character. Both slopes are influenced by pasture management. For the most comprehensive picture of Orthoptera we used a standard method (sweeping of vegetation and nonstandard methods (pitfall traps and Möricke yellow cups. Termophilous species of Orthoptera on Macošská stráň (47% were dominating. On Vilémovická stráň mezophilous species (46% were dominating. The most common species were Stenobothrus lineatus (Panzer, 1796 on Macošská stráň slope and Chorthippus parallelus (Zetterstedt, 1821, Stenobothrus lineatus, Chorthippus bigutulus (Linné, 1758 and Chorthippus dorsatus (Zetterstedt, 1821 on Vilémovická stráň slope. Rare species of this assemblage were Stenobothrus nigromaculatus (Herrich-Schaffer, 1840 which was on Macošská stráň slope only and Tetrix bipunctata (Linnaeus, 1758 which has much bigger abundances also on Macošská stráň slope.

  15. Um siðferðis- og skapgerðarmenntun: á óformlegt nám erindi inn í skóla?

    OpenAIRE

    Pálsdóttir, Kolbrún Þ.

    2017-01-01

    Þessi grein varpar ljósi á siðferðis- og skapgerðarmenntun innan skóla og stöðu slíkrar menntunar á Íslandi. Margt bendir til þess að þó að fræðimenn hafi á síðustu árum varpað ljósi á mikilvægi þess að efla siðferðilega dómgreind nemenda, sem og félags- og tilfinningaþroska þeirra, verði sá þáttur skólastarfsins iðulega útundan. Innleiðingu á lífsleikni sem námsgrein í grunn- og framhaldsskóla má skoða sem einn þátt í þeirri viðleitni að efla siðferðismenntun og heildstæða sýn...

  16. Inhibitions of mTORC1 and 4EBP-1 are key events orchestrated by Rottlerin in SK-Mel-28 cell killing.

    Science.gov (United States)

    Daveri, E; Maellaro, E; Valacchi, G; Ietta, F; Muscettola, M; Maioli, E

    2016-09-28

    Earlier studies demonstrated that Rottlerin exerts a time- and dose-dependent antiproliferative effect on SK-Mel-28 melanoma cells during 24 h of treatment, but cytotoxicity due to cell death began only after a 48 h exposure. In the current study, in order to identify the type of cell death in this cell line, which is notoriously refractory to most anticancer therapies, and to clarify the underlying mechanisms of this delayed outcome, we searched for apoptotic, necrotic/necroptotic and autophagic traits in Rottlerin-exposed cells. Although SK-Mel-28 cells are both apoptosis and autophagy competent, Western blotting analysis, caspase activity assay, nuclear imaging and the effects of autophagy, apoptosis and necroptosis inhibitors, indicated that Rottlerin cytotoxicity was due to none of the aforementioned death mechanisms. Nevertheless, in growth arrested cells, the death did occur after a prolonged treatment and most likely ensued from the observed blockage of protein synthesis that reached levels expected to be incompatible with cell survival. From a mechanistic point of view, we ascribed this effect to the documented inhibition of mTORC1 activity; mTORC1 inhibition on the one hand led to a not deadly, rather protective autophagic response but, on the other hand caused a near complete arrest of protein synthesis. Interestingly, no cytotoxicity was found towards normal skin fibroblasts, which only resulted mildly growth arrested by the drug. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  17. Process optimization and photostability of silymarin nanostructured lipid carriers: effect on UV-irradiated rat skin and SK-MEL 2 cell line.

    Science.gov (United States)

    Singh, Pooja; Singh, Mahendra; Kanoujia, Jovita; Arya, Malti; Saraf, Shailendra K; Saraf, Shubhini A

    2016-10-01

    The objective of the present work was to formulate a novel stable delivery system which would not only overcome the solubility issue of silymarin, but also help to increase the therapeutic value by better permeation, anticancer action and reduced toxicity. This was envisaged through the recent developments in nanotechnology, combined with the activity of the phytoconstituent silymarin. A 2(3) full factorial design based on three independent variables was used for process optimization of nanostructured lipid carriers (NLC). Developed formulations were evaluated on the basis of particle size, morphology, in vitro drug release, photostability and cell line studies. Optimized silymarin-NLC was incorporated into carbopol gel and further assessed for rheological parameters. Stable behaviour in presence of light was proven by photostability testing of formulation. Permeability parameters were significantly higher in NLC as compared to marketed phytosome formulation. The NLC based gel described in this study showed faster onset, and prolonged activity up to 24 h and better action against edema as compared to marketed formulation. In case of anticancer activity of silymarin-NLC against SK-MEL 2 cell lines, silymarin-NLC proved to possess anticancer activity in a dose-dependent manner (10-80 μM) and induced apoptosis at 80 μM in SK-MEL 2 cancer cells. This work documents for the first time that silymarin can be formulated into nanostructured lipoidal carrier system for enhanced permeation, greater stability as well as anticancer activity for skin.

  18. Neuroglobin overexpression plays a pivotal role in neuroprotection through mitochondrial raft-like microdomains in neuroblastoma SK-N-BE2 cells.

    Science.gov (United States)

    Garofalo, Tina; Ferri, Alberto; Sorice, Maurizio; Azmoon, Pardis; Grasso, Maria; Mattei, Vincenzo; Capozzi, Antonella; Manganelli, Valeria; Misasi, Roberta

    2018-04-01

    Since stressing conditions induce a relocalization of endogenous human neuroglobin (NGB) to mitochondria, this research is aimed to evaluate the protective role of NGB overexpression against neurotoxic stimuli, through mitochondrial lipid raft-associated complexes. To this purpose, we built a neuronal model of oxidative stress by the use of human dopaminergic neuroblastoma cells, SK-N-BE2, stably overexpressing NGB by transfection and treated with 1-methyl-4-phenylpyridinium ion (MPP+). We preliminary observed the redistribution of NGB to mitochondria following MPP+ treatment. The analysis of mitochondrial raft-like microdomains revealed that, following MPP+ treatment, NGB translocated to raft fractions (Triton X-100-insoluble), where it interacts with ganglioside GD3. Interestingly, the administration of agents capable of perturbating microdomain before MPP+ treatment, significantly affected viability in SK-N-BE2-NGB cells. The overexpression of NGB was able to abrogate the mitochondrial injuries on complex IV activity or mitochondrial morphology induced by MPP+ administration. The protective action of NGB on mitochondria only takes place if the mitochondrial lipid(s) rafts-like microdomains are intact, indeed NGB fails to protect complex IV activity when purified mitochondria were treated with the lipid rafts disruptor methyl-β-cyclodextrin. Thus, our unique in vitro model of stably transfected cells overexpressing endogenous NGB allowed us to suggest that the role in neuroprotection played by NGB is reliable only through interaction with mitochondrial lipid raft-associated complexes. Copyright © 2018 Elsevier Inc. All rights reserved.

  19. Elementary analysis and energetic potential of the municipal sewage sludges from the Gdańsk and Kościerzyna WWTPs

    Directory of Open Access Journals (Sweden)

    Ostojski Arkadiusz

    2018-01-01

    Full Text Available This paper aims to present municipal sewage sludge (MSS elementary analysis and energetic potential based on measurement of heat of combustion (higher heating value HHV and calculation of calorific values (lower heating value LHV. The analysis takes into the consideration water content in sewage sludge, at different utilization stages, in wastewater treatment plants in Gdańsk Wschód and Kościerzyna – Pomeranian Voivodeship. The study yielded the following results (in % dry matter: ash 19÷31 %, C - 31÷36 %, H - 5÷6 %, N - 4÷6 %, O - 28÷32 %, S – 1 %. Calorific value of stabilized sludges in Gdańsk was on average 13.8÷15 MJ/kg. In case of sludges not undergoing digestion from Kościerzyna WWTP, the calorific value was at the level of 17.5 MJ/kg. Thus, sewage sludges are good energy carriers. High water content though is the problem, as it lowers the useful effect of heat. There is no alternative for thermal sewage sludge neutralization, which is in conformity with valid Polish National Waste Management Plan (KPGO 2022.

  20. Elementary analysis and energetic potential of the municipal sewage sludges from the Gdańsk and Kościerzyna WWTPs

    Science.gov (United States)

    Ostojski, Arkadiusz

    2018-01-01

    This paper aims to present municipal sewage sludge (MSS) elementary analysis and energetic potential based on measurement of heat of combustion (higher heating value HHV) and calculation of calorific values (lower heating value LHV). The analysis takes into the consideration water content in sewage sludge, at different utilization stages, in wastewater treatment plants in Gdańsk Wschód and Kościerzyna - Pomeranian Voivodeship. The study yielded the following results (in % dry matter): ash 19÷31 %, C - 31÷36 %, H - 5÷6 %, N - 4÷6 %, O - 28÷32 %, S - 1 %. Calorific value of stabilized sludges in Gdańsk was on average 13.8÷15 MJ/kg. In case of sludges not undergoing digestion from Kościerzyna WWTP, the calorific value was at the level of 17.5 MJ/kg. Thus, sewage sludges are good energy carriers. High water content though is the problem, as it lowers the useful effect of heat. There is no alternative for thermal sewage sludge neutralization, which is in conformity with valid Polish National Waste Management Plan (KPGO 2022).

  1. A Model for Spheroid versus Monolayer Response of SK-N-SH Neuroblastoma Cells to Treatment with 15-Deoxy-PGJ2

    Directory of Open Access Journals (Sweden)

    Dorothy I. Wallace

    2016-01-01

    Full Text Available Researchers have observed that response of tumor cells to treatment varies depending on whether the cells are grown in monolayer, as in vitro spheroids or in vivo. This study uses data from the literature on monolayer treatment of SK-N-SH neuroblastoma cells with 15-deoxy-PGJ2 and couples it with data on growth rates for untreated SK-N-SH neuroblastoma cells grown as multicellular spheroids. A linear model is constructed for untreated and treated monolayer data sets, which is tuned to growth, death, and cell cycle data for the monolayer case for both control and treatment with 15-deoxy-PGJ2. The monolayer model is extended to a five-dimensional nonlinear model of in vitro tumor spheroid growth and treatment that includes compartments of the cell cycle (G1,S,G2/M as well as quiescent (Q and necrotic (N cells. Monolayer treatment data for 15-deoxy-PGJ2 is used to derive a prediction of spheroid response under similar treatments. For short periods of treatment, spheroid response is less pronounced than monolayer response. The simulations suggest that the difference in response to treatment of monolayer versus spheroid cultures observed in laboratory studies is a natural consequence of tumor spheroid physiology rather than any special resistance to treatment.

  2. Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells

    Directory of Open Access Journals (Sweden)

    Gang Li

    2014-01-01

    Full Text Available The aim of this study is to evaluate the protection of ginsenoside Rg3 against oxidative stress in human neuroblastoma SK-N-SH cells. 20(R-ginsenoside Rg3 (20(R-Rg3 and 20(S-ginsenoside Rg3 (20(S-Rg3 were prepared by the method of chemical degradation and column chromatography, and the structure of the two compounds was characterized by 1H-NMR and 13C-NMR spectroscopy. MTT assay and LDH leakage assay were used to determine the cell viability and the oxidative stress cellular model was established by means of H2O2 (600 μM for 4 h. We also investigated the changes of intracellular MDA content, SOD activity, and ROS formation after the treatment of ginsenoside Rg3 for 20 h. The results indicated that both 20 (R-Rg3 and 20 (S-Rg3 had obvious protection against H2O2-induced oxidative stress in SK-N-SH cells. Moreover, 20(R-Rg3 exhibited better antioxidant activity than 20(S-Rg3 in vitro. These findings are expected to provide some implication for further research and application of ginsenoside Rg3 in neuroprotection.

  3. The mojastin mutant Moj-DM induces apoptosis of the human melanoma SK-Mel-28, but not the mutant Moj-NN nor the non-mutated recombinant Moj-WN.

    Science.gov (United States)

    Seoane, Agustin I; Tran, Victoria L; Sanchez, Elda E; White, Stephanie A; Choi, Jason L; Gaytán, Brandon; Chavez, Natalie; Reyes, Steven R; Ramos, Carla J; Tran, Luan H; Lucena, Sara E; Sugarek, Maria; Perez, John C; Mandal, Stephanie A; Ghorab, Shervin; Rodriguez-Acosta, Alexis; Fung, Branden K; Soto, Julio G

    2010-09-01

    In this study, three recombinant mojastin peptides (Moj-WN, Moj-NN, and Moj-DM) were produced and compared functionally. Recombinant Moj peptides were purified as GST-fusions. GST-Moj-WN and GST-Moj-NN inhibited ADP-induced platelet aggregation in platelet rich plasma. The GST-Moj-WN had an IC(50) of 160nM, while the GST-Moj-NN had an IC(50) of 493nM. The GST-Moj-DM did not inhibit platelet aggregation. All three GST-Moj peptides inhibited SK-Mel-28 cell adhesion to fibronectin. The GST-Moj-WN inhibited the binding of SK-Mel-28 cells to fibronectin with an IC(50) of 11nM, followed by the GST-Moj-NN (IC(50) of 28nM), and the GST-Moj-DM (IC(50) of 46nM). The GST-Moj peptides' ability to induce apoptosis on SK-Mel-28 cells was determined using Annexin-V-FITC and nuclear fragmentation assays. Cells were incubated with 5muM GST-Moj peptides for 24h. At 5microM GST-Moj-DM peptide, 13.56%+/-2.08 of treated SK-Mel-28 cells were in early apoptosis. The GST-Moj-DM peptide also caused nuclear fragmentation as determined by fluorescent microscopy and Hoechst staining. The GST-Moj-WN and GST-Moj-NN peptides failed to induce apoptosis. We characterized the SK-Mel-28 integrin expression, as the first step in determining r-Moj binding specificity. Our results indicate that SK-Mel-28 cells express alphavbeta3, alphav, alpha6, beta1, and beta3 integrin receptors. Published by Elsevier Ltd.

  4. TIMP-1 overexpression does not affect sensitivity to HER2-targeting drugs in the HER2-gene-amplified SK-BR-3 human breast cancer cell line

    DEFF Research Database (Denmark)

    Deng, Xiaohong; Fogh, Louise; Lademann, Ulrik Axel

    2013-01-01

    affect sensitivity to the HER2-targeting drugs trastuzumab and lapatinib. SK-BR-3 human breast cancer cells were stably transfected with TIMP-1, characterized with regard to TIMP-1 protein expression, proliferation, and functionality of the secreted TIMP-1, and the sensitivity to trastuzumab...... and lapatinib was studied in five selected single-cell subclones expressing TIMP-1 protein at various levels plus the parental SK-BR-3 cell line. Both trastuzumab and lapatinib reduced cell viability, as determined by MTT assay, but the sensitivity to the drugs was not associated with the expression level...... to trastuzumab and lapatinib....

  5. Wytrzymałość na ścinanie odpadów komunalnych a stateczność skarp składowisk

    Directory of Open Access Journals (Sweden)

    Tymoteusz Zydroń

    2015-12-01

    Full Text Available W pierwszej części pracy dokonano analizy literatury tematycznej dotyczącej parametrów charakteryzujących wytrzymałość na ścinanie odpadów komunalnych. Wykazała ona, że odpady komunalne charakteryzują się bardzo zróżnicowanymi warto­ściami kąta tarcia wewnętrznego i spójności, które zależą m.in. od ich struktury, stopnia rozkładu, metody badań, w tym zakresu odkształceń. Wpływ tych czynników na wytrzyma­łość na ścinanie odpadów jest często niejednoznaczny, co powoduje, że dobór parametrów wytrzymałościowych dla prognozowania stateczności składowisk jest zadaniem złożonym. W drugiej części pracy dokonano obliczeń stateczności skarpy kwatery składowiska od­ padów komunalnych w Chełmku, wykorzystując wartości parametrów wytrzymałościowych przyjętych z przedmiotowej literatury. Wykazano, że tradycyjna metoda obliczeń stateczności skarpy składowiska, z zastosowaniem uśrednionych parametrów wytrzyma­łościowych odpadów, dała wysoką wartość współczynnika stateczności, natomiast analiza probabilistyczna wykazała, że bezpieczeństwo konstrukcji można uznać jako sytuujące się pomiędzy słabym, a przeciętnym.

  6. Analysis and Risk Evaluation on the Case of Alteration, Revitalization and Conversion of a Historic Building in Gdańsk

    Science.gov (United States)

    Grzyl, Beata; Kristowski, Adam; Miszewska-Urbańska, Emilia

    2017-10-01

    Each investment plan, including the one concerning a building, is exposed to the consequences of various types of threats taking place. Therefore, in the case of some large-scale, atypical and complicated building ventures, some actions included in the procedure of risk management should be taken (identifications, analysis, measurements, control and supervision of the risk). This will allow for the risk to be eliminated or limited. While preparing a building venture, an investor does not possess full information about the course of events on each stage of investment completion. The identification of the above-mentioned unknowns, subjecting them to quantification and specifying the method of dealing with them, allows an investor to increase the effectiveness of the intended plan. The enterprise discussed in this article and analyzed in the context of risk, concerns alteration, revitalization and conversion for office purposes of two buildings located in Gdańsk at 1 and 2 Lastadia Street. These buildings are situated on the area of historical urban layout of Gdańsk, in the northern-eastern part of Stare Przedmieście District (Old Suburb), about 800 meters south from Dlugi Targ Street and 200 meters west from The Old Motława River. The investor is “Gdańskie Melioracje Ltd.”, a limited liability company, which belongs to the Council of Gdańsk. In order to increase the effectiveness of the intended investment venture, while organizing the investment process, the investor commissioned preparation of an analysis and risk evaluation connected with the above-mentioned intention. Based on an on-site visit, the opinions of experts, who have been involved in the process of the preparation of the investment, studies of the available monographies about the technical condition of the buildings at 1 and 2 Lastadia Street and their own experiences, the authors identified 54 types of relevant risks, which have been systematized into 10 subject groups (among others

  7. Survivin knockdown increased anti-cancer effects of (-)-epigallocatechin-3-gallate in human malignant neuroblastoma SK-N-BE2 and SH-SY5Y cells.

    Science.gov (United States)

    Hossain, Md Motarab; Banik, Naren L; Ray, Swapan K

    2012-08-01

    Neuroblastoma is a solid tumor that mostly occurs in children. Malignant neuroblastomas have poor prognosis because conventional chemotherapeutic agents are hardly effective. Survivin, which is highly expressed in some malignant neuroblastomas, plays a significant role in inhibiting differentiation and apoptosis and promoting cell proliferation, invasion, and angiogenesis. We examined consequences of survivin knockdown by survivin short hairpin RNA (shRNA) plasmid and then treatment with (-)-epigallocatechin-3-gallate (EGCG), a green tea flavonoid, in malignant neuroblastoma cells. Our Western blotting and laser scanning confocal immunofluorescence microscopy showed that survivin was highly expressed in malignant neuroblastoma SK-N-BE2 and SH-SY5Y cell lines and slightly in SK-N-DZ cell line. Expression of survivin was very faint in malignant neuroblastoma IMR32 cell line. We transfected SK-N-BE2 and SH-SY-5Y cells with survivin shRNA, treated with EGCG, and confirmed knockdown of survivin at mRNA and protein levels. Survivin knockdown induced morphological features of neuronal differentiation, as we observed following in situ methylene blue staining. Combination of survivin shRNA and EGCG promoted neuronal differentiation biochemically by increases in the expression of NFP, NSE, and e-cadherin and also decreases in the expression of Notch-1, ID2, hTERT, and PCNA. Our in situ Wright staining and Annexin V-FITC/PI staining showed that combination therapy was highly effective in inducing, respectively, morphological and biochemical features of apoptosis. Apoptosis occurred with activation of caspase-8 and cleavage of Bid to tBid, increase in Bax:Bcl-2 ratio, mitochondrial release of cytochrome c, and increases in the expression and activity of calpain and caspase-3. Combination therapy decreased migration of cells through matrigel and inhibited proliferative (p-Akt and NF-κB), invasive (MMP-2 and MMP-9), and angiogenic (VEGF and b-FGF) factors. Also, in vitro

  8. Survivin knockdown increased anti-cancer effects of (-)-epigallocatechin-3-gallate in human malignant neuroblastoma SK-N-BE2 and SH-SY5Y cells

    Energy Technology Data Exchange (ETDEWEB)

    Hossain, Md. Motarab [Department of Pathology, Microbiology, and Immunology, University of South Carolina School of Medicine, Columbia, SC (United States); Banik, Naren L. [Department of Neurosciences, Medical University of South Carolina, Charleston, SC (United States); Ray, Swapan K., E-mail: swapan.ray@uscmed.sc.edu [Department of Pathology, Microbiology, and Immunology, University of South Carolina School of Medicine, Columbia, SC (United States)

    2012-08-01

    Neuroblastoma is a solid tumor that mostly occurs in children. Malignant neuroblastomas have poor prognosis because conventional chemotherapeutic agents are hardly effective. Survivin, which is highly expressed in some malignant neuroblastomas, plays a significant role in inhibiting differentiation and apoptosis and promoting cell proliferation, invasion, and angiogenesis. We examined consequences of survivin knockdown by survivin short hairpin RNA (shRNA) plasmid and then treatment with (-)-epigallocatechin-3-gallate (EGCG), a green tea flavonoid, in malignant neuroblastoma cells. Our Western blotting and laser scanning confocal immunofluorescence microscopy showed that survivin was highly expressed in malignant neuroblastoma SK-N-BE2 and SH-SY5Y cell lines and slightly in SK-N-DZ cell line. Expression of survivin was very faint in malignant neuroblastoma IMR32 cell line. We transfected SK-N-BE2 and SH-SY-5Y cells with survivin shRNA, treated with EGCG, and confirmed knockdown of survivin at mRNA and protein levels. Survivin knockdown induced morphological features of neuronal differentiation, as we observed following in situ methylene blue staining. Combination of survivin shRNA and EGCG promoted neuronal differentiation biochemically by increases in the expression of NFP, NSE, and e-cadherin and also decreases in the expression of Notch-1, ID2, hTERT, and PCNA. Our in situ Wright staining and Annexin V-FITC/PI staining showed that combination therapy was highly effective in inducing, respectively, morphological and biochemical features of apoptosis. Apoptosis occurred with activation of caspase-8 and cleavage of Bid to tBid, increase in Bax:Bcl-2 ratio, mitochondrial release of cytochrome c, and increases in the expression and activity of calpain and caspase-3. Combination therapy decreased migration of cells through matrigel and inhibited proliferative (p-Akt and NF-{kappa}B), invasive (MMP-2 and MMP-9), and angiogenic (VEGF and b-FGF) factors. Also, in vitro

  9. Non-native crab Rhithropanopeus harrisii (Gould, 1984 - a new component of the benthic communities in the Gulf of Gdańsk (southern Baltic Sea

    Directory of Open Access Journals (Sweden)

    Joanna Hegele-Drywa

    2014-01-01

    Full Text Available The aim of this study was to determine the occurrence, spatial distribution and abundance pattern of the Harris mud crab Rhithropanopeus harrisii in the Gulf of Gdańsk (southern Baltic Sea. Between 2006 and 2010, this species was found at 69 out of 129 sampling stations, at depths from 0 to 20 m. Two main aggregations of the Harris mud crab were established: (1 in Puck Bay (max. density 19 indiv. 100 m-2 and (2 in the Gdynia and Sopot area (max. density 5 indiv. 100 m-2. 920 specimens were collected during the whole sampling period: 150 juveniles, 370 females and 400 males. The minimum measured carapace width was 1.96 mm, the maximum 21.40 mm (mean 9.03 ±4.11 mm.

  10. 3'-Hydroxy-3,4'-dimethoxyflavone blocks tubulin polymerization and is a potent apoptotic inducer in human SK-MEL-1 melanoma cells.

    Science.gov (United States)

    Estévez-Sarmiento, Francisco; Said, Mercedes; Brouard, Ignacio; León, Francisco; García, Celina; Quintana, José; Estévez, Francisco

    2017-11-01

    Flavonoids are naturally occurring polyphenolic compounds and are among the most promising anticancer agents. A series of flavonols and their 3-methyl ether derivatives were synthesized and assessed for cytotoxicity. It was found that 3'-hydroxy-3,4'-dimethoxyflavone (flavonoid 7a) displayed strong cytotoxicity against human SK-MEL-1 melanoma cells and blocked tubulin polymerization, but had no significant cytotoxic effects against quiescent or proliferating human peripheral blood mononuclear cells. Our analyses showed that flavonoid 7a induces G 2 -M cell cycle arrest and apoptosis in melanoma cells which is associated with cytochrome c release and activation of both extrinsic and intrinsic apoptotic pathways of cell death. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. ‘Close to the city centre, close to the university’. Are there symptoms of studentification in Gdańsk, Poland?

    Directory of Open Access Journals (Sweden)

    Grabkowska Maja

    2016-06-01

    Full Text Available Students are increasingly recognised as important actors of urban change in contemporary cities. The article presents an analysis of incipient studentification processes in Gdańsk. Its general aim is to reveal patterns of students’ presence and activity which translate into spatial and socio-economic transformations of a post-socialist city at the level of neighbourhoods. The study consists of a survey on students’ residential behaviour, complemented by field and desk research. According to the results, due to the fact that Polish students’ housing and lifestyle choices are limited by their low purchasing power, student-dedicated services have a rather negative impact on the quality of urban space. Furthermore, under these conditions studentification may not be regarded as a stimulus to the gentrification process, which is rather driven by hipsters and affluent foreign students.

  12. A recurrent human papillomavirus integration site at chromosome region 12q14-q15 in SW756 and SK-v cell lines derived from genital tumors

    International Nuclear Information System (INIS)

    Sastre-Garau, X.; Couturier, J.; Favre, M.; Orth, G.

    1995-01-01

    The SW756 cell line, derived from an invasive cancer of the uterine cervix, harbours integrated human papillomavirus (HPV) 18 DNA sequences which have been located in chromosome band 12q13. By in situ hybridization experiments with tritiated and digoxigenin-labelled HPV18 probes on R-banded chromosomes, we now localize the integrated viral sequences in 12q14-q15. Interestingly, we have previously localized integrated HPV16 sequences in the same chromosomal region in SK-v cells, derived from a pre-invasive vulvar neoplasia. The chromosomal region 12q14-q15 could thus correspond to a preferential site for the integration of HPV DNA in genital tumors. (authors). 29 refs., 2 figs

  13. Determination of In-situ Rock Thermal Properties from Geophysical Log Data of SK-2 East Borehole, Continental Scientific Drilling Project of Songliao Basin, NE China

    Science.gov (United States)

    Zou, C.; Zhao, J.; Zhang, X.; Peng, C.; Zhang, S.

    2017-12-01

    Continental Scientific Drilling Project of Songliao Basin is a drilling project under the framework of ICDP. It aims at detecting Cretaceous environmental/climate changes and exploring potential resources near or beneath the base of the basin. The main hole, SK-2 East Borehole, has been drilled to penetrate through the Cretaceous formation. A variety of geophysical log data were collected from the borehole, which provide a great opportunity to analyze thermal properties of in-situ rock surrounding the borehole.The geothermal gradients were derived directly from temperature logs recorded 41 days after shut-in. The matrix and bulk thermal conductivity of rock were calculated with the geometric-mean model, in which mineral/rock contents and porosity were required as inputs (Fuchs et. al., 2014). Accurate mineral contents were available from the elemental capture spectroscopy logs and porosity data were derived from conventional logs (density, neutron and sonic). The heat production data were calculated by means of the concentrations of uranium, thorium and potassium determined from natural gamma-ray spectroscopy logs. Then, the heat flow was determined by using the values of geothermal gradients and thermal conductivity.The thermal parameters of in-situ rock over the depth interval of 0 4500m in the borehole were derived from geophysical logs. Statistically, the numerical ranges of thermal parameters are in good agreement with the measured values from both laboratory and field in this area. The results show that high geothermal gradient and heat flow exist over the whole Cretaceous formation, with anomalously high values in the Qingshankou formation (1372.0 1671.7m) and the Quantou formation (1671.7 2533.5m). It is meaningful for characterization of geothermal regime and exploration of geothermal resources in the basin. Acknowledgment: This work was supported by the "China Continental Scientific Drilling Program of Cretaceous Songliao Basin (CCSD-SK)" of China

  14. Modulation of notch signaling pathway to prevent H2O2/menadione-induced SK-N-MC cells death by EUK134.

    Science.gov (United States)

    Kamarehei, Maryam; Yazdanparast, Razieh

    2014-10-01

    The brain in Alzheimer's disease is under increased oxidative stress, and this may have a role in the pathogenesis and neural death in this disorder. It has been verified that numerous signaling pathways involved in neurodegenerative disorders are activated in response to reactive oxygen species (ROS). EUK134, a synthetic salen-manganese antioxidant complex, has been found to possess many interesting pharmacological activities awaiting exploration. The present study is to characterize the role of Notch signaling in apoptotic cell death of SK-N-MC cells. The cells were treated with hydrogen peroxide (H2O2) or menadione to induce oxidative stress. The free-radical scavenging capabilities of EUK134 were studied through the MTT assay, glutathione peroxidase (GPx) enzyme activity assay, and glutathione (GSH) Levels. The extents of lipid peroxidation, protein carbonyl formation, and intracellular ROS levels, as markers of oxidative stress, were also studied. Our results showed that H2O2/menadione reduced GSH levels and GPx activity. However, EUK134 protected cells against ROS-induced cell death by down-regulation of lipid peroxidation and protein carbonyl formation as well as restoration of antioxidant enzymes activity. ROS induced apoptosis and increased NICD and HES1 expression. Inhibition of NICD production proved that Notch signaling is involved in apoptosis through p53 activation. Moreover, H2O2/menadione led to Numb protein down-regulation which upon EUK134 pretreatment, its level increased and subsequently prevented Notch pathway activation. We indicated that EUK134 can be a promising candidate in designing natural-based drugs for ROS-induced neurodegenerative diseases. Collectively, ROS activated Notch signaling in SK-N-MC cells leading to cell apoptosis.

  15. Human T-lymphotropic virus type 1 Tax protein complexes with P-TEFb and competes for Brd4 and 7SK snRNP/HEXIM1 binding.

    Science.gov (United States)

    Cho, Won-Kyung; Jang, Moon Kyoo; Huang, Keven; Pise-Masison, Cynthia A; Brady, John N

    2010-12-01

    Positive transcription elongation factor b (P-TEFb) plays an important role in stimulating RNA polymerase II elongation for viral and cellular gene expression. P-TEFb is found in cells in either an active, low-molecular-weight (LMW) form or an inactive, high-molecular-weight (HMW) form. We report here that human T-lymphotropic virus type 1 (HTLV-1) Tax interacts with the cyclin T1 subunit of P-TEFb, forming a distinct Tax/P-TEFb LMW complex. We demonstrate that Tax can play a role in regulating the amount of HMW complex present in the cell by decreasing the binding of 7SK snRNP/HEXIM1 to P-TEFb. This is seen both in vitro using purified Tax protein and in vivo in cells transduced with Tax expression constructs. Further, we find that a peptide of cyclin T1 spanning the Tax binding domain inhibits the ability of Tax to disrupt HMW P-TEFb complexes. These results suggest that the direct interaction of Tax with cyclin T1 can dissociate P-TEFb from the P-TEFb/7SK snRNP/HEXIM1 complex for activation of the viral long terminal repeat (LTR). We also show that Tax competes with Brd4 for P-TEFb binding. Chromatin immunoprecipitation (ChIP) assays demonstrated that Brd4 and P-TEFb are associated with the basal HTLV-1 LTR, while Tax and P-TEFb are associated with the activated template. Furthermore, the knockdown of Brd4 by small interfering RNA (siRNA) activates the HTLV-1 LTR promoter, which results in an increase in viral expression and production. Our studies have identified Tax as a regulator of P-TEFb that is capable of affecting the balance between its association with the large inactive complex and the small active complex.

  16. Associations between cognitive abilities and life satisfaction in the oldest-old. Results from the longitudinal population study Good Aging in Skåne

    Directory of Open Access Journals (Sweden)

    Enkvist Å

    2013-07-01

    Full Text Available Åsa Enkvist, Henrik Ekström, Sölve Elmståhl Department of Health Sciences, Division of Geriatric Medicine, Lund University, Skåne University Hospital, Malmö, Sweden Introduction: Studies on the associations between cognitive abilities and life satisfaction (LS in the oldest-old are few. The aim of this study was to explore whether abilities in six different cognitive domains could predict LS in the oldest-old 3 years later. Methods: The study population consisted of 681 individuals aged 78–98 years, drawn from the longitudinal population study “Good Aging in Skåne,” which is part of a national survey (The Swedish National Study on Aging and Care. Scores on 13 cognitive tests were related to scores on Neugartens’ LS index A (LSI-A 3 years later. The cognitive tests were added into six different cognitive domains. A multiple regression analysis was constructed for each cognitive domain separately, with scores on the LSI-A as the dependent variable. The model was adjusted stepwise for sex, age, education, functional capacity, and depressive mood. Results: Significant correlations were found between digit cancellation, word recall, verbal fluency (VF A, VF animals, VF occupations, and mental rotations at baseline, as well as LSI-A at follow-up. The domains of spatial abilities (B = 0.453, P = 0.014 and processing speed (B = 0.118, P = 0.020 remained significantly associated with LSI-A 3 years later after adjustment. Conclusion: The cognitive domains of spatial abilities and processing speed predicted LS 3 years later in the oldest-old. Clinical implications are discussed. Keywords: oldest-old, life satisfaction, longitudinal, crystallized and fluid intelligence, cognition

  17. Lycopene inhibits reactive oxygen species production in SK-Hep-1 cells and attenuates acetaminophen-induced liver injury in C57BL/6 mice.

    Science.gov (United States)

    Bandeira, Ana Carla Balthar; da Silva, Talita Prato; de Araujo, Glaucy Rodrigues; Araujo, Carolina Morais; da Silva, Rafaella Cecília; Lima, Wanderson Geraldo; Bezerra, Frank Silva; Costa, Daniela Caldeira

    2017-02-01

    Our aim was to investigate the antioxidant potential of lycopene in different experimental liver models: in vitro, to evaluate the influence of lycopene on reactive oxygen species (ROS) production mediated by the PKC pathway and in vivo, to evaluate the protective effects of lycopene in an experimental model of hepatotoxicity. The in vitro study assessed the lycopene antioxidant potential by the quantification of ROS production in SK-Hep-1 cells unstimulated or stimulated by an activator of the PKC pathway. The role of NADPH oxidase was evaluated by measuring its inhibition potential using an inhibitor of this enzyme. In the in vivo study, male C57BL/6 mice received lycopene (10 or 100 mg/kg by oral gavage) and 1 h later, acetaminophen (APAP) (500 mg/kg) was administrated. Lycopene decreased ROS production in SK-Hep-1 cells through inhibition of NADPH oxidase, brought about in the PKC pathway. Lycopene improved hepatotoxicity acting as an antioxidant, reduced GSSG and regulated tGSH and CAT levels, reduced oxidative damage primarily by decreasing protein carbonylation, promoted the downregulation of MMP-2 and reduced areas of necrosis improving the general appearance of the lesion in C57BL/6 mice. Lycopene is a natural compound that was able to inhibit the production of ROS in vitro and mitigate the damage caused by APAP overdose in vivo. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  18. [Prevalence of the Diabetic Retinopathy and Genetic Factors Significance in the Development of Diabetic Retinopathy in Patients with Diabetes Mellitus type I and II in Slovakia (DIARET SK study). Overview of Actual Findings and Design of the Epidemiological DIARET SK Study].

    Science.gov (United States)

    Krásnik, V; Štefaničková, J; Fabková, J; Bucková, D; Helbich, M

    2015-09-01

    Diabetic retinopathy (DR) is the second most common microvascular complication and the most common cause of blindness in patients with diabetes mellitus (DM). Despite the ongoing research, the findings of diabetic retinopathy epidemiological and risk factors are, until now, not consistent. More finding may be revealed by epidemiological studies, consistently mapping DR epidemiology under the current possibilities of investigations and treatment of the DM. DIARET SK Study, with 5 000 enrolled patients with diabetes mellitus in the Slovak Republic, is, until now, the largest epidemiological study to set the prevalence of diabetic retinopathy. The primary aim is to establish the prevalence of diabetic retinopathy in patients with diabetes mellitus type I and II, according to the duration of the disease. The secondary aim is to establish prevalence of the different stages of the DR and diabetic macular edema (DME) and analysis of the risk factors influence. Included are patients with DM type I and II regardless to the ocular complications history and the period of DM duration. Each enrolled patient has both complex diabetic and ophthalmic examinations.Projects to establish DR prevalence: Tens of projects concerned with diabetic retinopathy epidemiology with different approaches to establish the prevalence and with different patients population. Results from different studies vary significantly (from 12.3 % to 66.9 %). The results depend on the design of the study and the patients recruitment, used examination methods, specific patients population with regard to the geography, prevalence of risk factors, period of diabetes duration, glycated hemoglobin (HbA1C) level, blood pressure, and is higher in type I diabetic patients. The most accurate results are from population epidemiological studies with well-controlled patient recruitment and uniform complex examination that are similar to the DIARET SK study. The DIARET SK study represents the largest epidemiological study

  19. Stržový systém v Bosonožském hájku – jedinečný geomorfologický fenomén západně od Brna

    Czech Academy of Sciences Publication Activity Database

    Kirchner, Karel; Münster, P.; Máčka, Z.

    2011-01-01

    Roč. 18, č. 2 (2011), s. 32-35 ISSN 1212-6209 Institutional research plan: CEZ:AV0Z30860518 Keywords : gully networks * loess * Bosonožský hájek area * Brno region Subject RIV: DE - Earth Magnetism, Geodesy, Geography

  20. Zircon and baddeleyite from the economic ultramafic-mafic Noril'sk-1 intrusion (Russia): Hf-isotope constraints on source composition

    Science.gov (United States)

    Malitch, K. N.; Belousova, E. A.; Badanina, I. Yu.; Griffin, W. L.

    2012-04-01

    The ultramafic-mafic Noril'sk-1 intrusion in the northwestern part of the Siberian Craton (Russia) represents one of three known Noril'sk-type, ore-bearing intrusions, which host one of the world's major economic sulphide platinum-group-element (PGE)-Cu-Ni deposits. Zircon and baddeleyite dated previously both by SHRIMP (i.e. 248.0 ± 3.7 Ma, Campbell et al. 1992) and ID-TIMS (251.1 ± 3.6 Ma, Kamo et al. 1996) have been restricted to one lithology (e.g. leucogabbro) of the Noril'sk-1 intrusion. To better constrain the age of igneous event and sources involved in its generation our multi-technique study utilized ten rock samples characteristic of unmineralized and mineralized lithologies. The rocks investigated comprise (from top to bottom) gabbro-diorite (sample N1-1), leucogabbro (N1-3), olivine-free gabbro (N1-2 and N1-4), olivine-bearing gabbro (N1-5), olivine gabbro (N1-6), plagiowehrlite and plagiodunite (N1-7), taxitic-textured rocks comprising melanotroctolite, olivine gabbro with relics of ultramafic rocks (N1-8, N1-9) and contact fine-grained gabbro (N1-10). Sulphide PGE-Cu-Ni ores occur in ultramafic (N1-7) and taxitic-textured rocks (N1-8 and N1-9), which have thickness of about 17 m, whereas the low-sulphide horizon of about 1 m thick occurs in the upper part of intrusion (N1-3). In situ U-Pb analyses of zircon from these rocks, combined with detailed study of crystal morphology and internal structure, identify four zircon populations (Malitch et al. 2012). The U-Pb ages of baddeleyite and the defined zircon populations cover a significant time span, from Late Paleozoic to Early Mesozoic (e.g., 290 ± 2.8; 261.3 ± 1.6; 245.7 ± 1.1; 236.5 ± 1.8 and 226.7 ± 0.9 Ma). The established distribution of U-Pb ages implies that crystallization of baddeleyite and zircon corresponds to several stages of protracted evolution of ultramafic-mafic magmas at deep-seated staging chambers and/or probably characterizes interaction of distinct magmas during formation

  1. Cultural Identity of Citizens of Gdańsk from an Ethnolinguistic Perspective on the Basis of Chosen Texts of the Free City of Danzig

    Directory of Open Access Journals (Sweden)

    Izabela Olszewska

    2015-06-01

    Full Text Available Cultural Identity of Citizens of Gdańsk from an Ethnolinguistic Perspective on the Basis of Chosen Texts of the Free City of Danzig As a consequence of the First World War and in the wake of the Treaty of Versailles the Free City of Danzig was established. The image of Danzig identity was created, first of all, by the following ethnic groups: German, Polish and Jewish. Was the Free City of Danzig multicultural or was it German with Polish and Jewish minorities? Did those nationalities live beside each other, did they together, as citizens of Danzig, create a common reality? Is it possible to talk about a uniform culture/identity of Danzig? The aim of this article is an analysis of linguistic image of the world of the citizens of the Free City of Danzig, which has been carried out on the basis of characteristics of the image of relations and cultural differences in the interpretation of Polish community. Yet, the whole image of identity consists of a mosaic of smaller interpretations which, only after being reduced to the lowest common denominator, may give the holistic image of Danzig identity as an image of a common multiple subjectivity. Mutual perception of nationalities inhabiting the area of the Free City of Danzig is the starting point for the discussion on their identity and an attempt to answer the question: did the citizens of Danzig see themselves as one community – exactly the one of Danzig? The analysed research material consists of chosen texts of the local press (in Polish and occasionally in German concerning socio-cultural and political and informative issues. The Polish "Gazeta Gdańska" had been published since 1891 as the first Polish Danzig newspaper in Polish. Its first aim was to integrate Polish circles and to defend the rights of the Polish. "Danziger Neuesten Nachrichten", published since 1894, was the biggest Danzig conservative newspaper in German which influenced public opinion. In the analysis of the linguistic

  2. Między brakiem a nadmiarem. Z Ireną Grudzińską-Gross rozmawia Joanna Tokarska-Bakir

    Directory of Open Access Journals (Sweden)

    Joanna Tokarska-Bakir

    2012-12-01

    Full Text Available Between overabundance and scarcity. Joanna Tokarska-Bakir in an interview with Irena Grudzińska Gross This conversation with Irena Grudzińska-Gross starts a series of interviews in “Studia Litteraria et Historica”, which motto is the title of the essay of Jan Stanisław Bystroń “On subjects I was advised not to pursue”. Among these subjects that Irena Grudzińska-Gross names are: war, family history, women.  Grudzińska-Gross tells also about the reactions to “Złote Żniwa” - a book that she wrote together with Jan Tomasz Gross, and about academic work of people of Eastern Europe living in the United States. One part of the interview concerns also a “collective hypnosis”, that Americans underwent after the 9/11 World Trade Center Attack and the price, that Susan Sontag had to pay for being critical about American militarism.   Między brakiem a nadmiarem. Z Ireną Grudzińską-Gross rozmawia Joanna Tokarska-Bakir Rozmowa z Ireną Grudzińską-Gross otwiera cykl wywiadów “Studia Litteraria et Historica”, których mottem jest tytuł eseju Jana Stanisława Bystronia: “Tematy, które mi odradzano”. Wśród “odradzanych” tematów, które wymienia Irena Grudzińska-Gross są między innymi: wojna, historia rodzinna, kobiety. Grudzińska-Gross opowiada także o reakcjach na książkę “Złote żniwa”, którą napisała wraz z Janem Tomaszem Grossem oraz o pracy akademickiej osób z Europy Wschodniej w Stanach Zjednoczonych. Część rozmowy dotyczy także “zbiorowej hipnozy”, której ulegli mieszkańcy USA po ataku 11 września 2001 na World Trade Center i ceny, jaką musiała zapłacić Susan Sontag za krytykę amerykańskiego militaryzmu.

  3. Neuroprotection via RNA-binding protein RBM3 expression is regulated by hypothermia but not by hypoxia in human SK-N-SH neurons

    Directory of Open Access Journals (Sweden)

    Rosenthal LM

    2017-05-01

    Full Text Available Lisa-Maria Rosenthal,1 Giang Tong,1 Christoph Walker,1 Sylvia J Wowro,1 Jana Krech,1 Constanze Pfitzer,1,2 Georgia Justus,1 Felix Berger,1,3 Katharina Rose Luise Schmitt1 1Department of Congenital Heart Disease/Pediatric Cardiology, German Heart Institute Berlin, 2Berlin Institute of Health (BIH, 3Department of Pediatric Cardiology, Charité – University Medical Center, Berlin, Germany Objective: Therapeutic hypothermia is an established treatment for perinatal asphyxia. Yet, many term infants continue to die or suffer from neurodevelopmental disability. Several experimental studies have demonstrated a beneficial effect of mild-to-moderate hypothermia after hypoxic injury, but the understanding of hypothermia-induced neuroprotection remains incomplete. In general, global protein synthesis is attenuated by hypothermia, but a small group of RNA-binding proteins including the RNA-binding motif 3 (RBM3 is upregulated in response to cooling. The aim of this study was to establish an in vitro model to investigate the effects of hypoxia and hypothermia on neuronal cell survival, as well as to examine the kinetics of concurrent cold-shock protein RBM3 gene expression. Methods: Experiments were performed by using human SK-N-SH neurons exposed to different oxygen concentrations (21%, 8%, or 0.2% O2 for 24 hours followed by moderate hypothermia (33.5°C or normothermia for 24, 48, or 72 hours. Cell death was determined by quantification of lactate dehydrogenase and neuron-specific enolase releases into the cell cultured medium, and cell morphology was assessed by using immunofluorescence staining. The regulation of RBM3 gene expression was assessed by reverse transcriptase-quantitative polymerase chain reaction and Western blot analysis.Results: Exposure to hypoxia (0.2% O2 for 24 hours resulted in significantly increased cell death in SK-N-SH neurons, whereas exposure to 8% O2 had no significant impact on cell viability. Post-hypoxia treatment with

  4. P2Y receptor-mediated transient relaxation of rat longitudinal ileum preparations involves phospholipase C activation, intracellular Ca(2+) release and SK channel activation.

    Science.gov (United States)

    Mader, Felix; Krause, Ludwig; Tokay, Tursonjan; Hakenberg, Oliver W; Köhling, Rüdiger; Kirschstein, Timo

    2016-05-01

    Purinergic signaling plays a major role in the enteric nervous system, where it governs gut motility through a number of P2X and P2Y receptors. The aim of this study was to investigate the P2Y receptor-mediated motility in rat longitudinal ileum preparations. Ileum smooth muscle strips were prepared from rats, and fixed in an organ bath. Isometric contraction and relaxation responses of the muscle strips were measured with force transducers. Drugs were applied by adding of stock solutions to the organ bath to yield the individual final concentrations. Application of the non-hydrolyzable P2 receptor agonists α,β-Me-ATP or 2-Me-S-ADP (10, 100 μmol/L) dose-dependently elicited a transient relaxation response followed by a sustained contraction. The relaxation response was largely blocked by SK channel blockers apamin (500 nmol/L) and UCL1684 (10 μmol/L), PLC inhibitor U73122 (100 μmol/L), IP3 receptor blocker 2-APB (100 μmol/L) or sarcoendoplasmic Ca(2+) ATPase inhibitor thapsigargin (1 μmol/L), but not affected by atropine, NO synthase blocker L-NAME or tetrodotoxin. Furthermore, α,β-Me-ATP-induced relaxation was suppressed by P2Y1 receptor antagonist MRS2179 (50 μmol/L) or P2Y13 receptor antagonist MRS2211 (100 μmol/L), and was abolished by co-application of the two antagonists, whereas 2-Me-S-ADP-induced relaxation was abolished by P2Y6 receptor antagonist MRS2578 (50 μmol/L). In addition, P2Y1 receptor antagonist MRS2500 (1 μmol/L) not only abolished α,β-Me-ATP-induced relaxation, but also suppressed 2-Me-S-ADP-induced relaxation. P2Y receptor agonist-induced transient relaxation of rat ileum smooth muscle strips is mediated predominantly by P2Y1 receptor, but also by P2Y6 and P2Y13 receptors, and involves PLC, IP3, Ca(2+) release and SK channel activation, but is independent of acetylcholine and NO release.

  5. Heat shock protein 90 inhibitor enhances apoptosis by inhibiting the AKT pathway in thermal-stimulated SK-MEL-2 human melanoma cell line.

    Science.gov (United States)

    Shin, Min Kyung; Jeong, Ki-Heon; Choi, Hyeongwon; Ahn, Hye-Jin; Lee, Mu-Hyoung

    2018-02-08

    Heat shock proteins (Hsps) are chaperone proteins, which are upregulated after various stresses. Hsp90 inhibitors have been investigated as adjuvant therapies for the treatment of melanoma. Thermal ablation could be a treatment option for surgically unresectable melanoma or congenital nevomelanocytic nevi, however, there is a limitation such as the possibility of recurrence. We evaluated apoptosis in a melanoma cell line treated with the Hsp90 inhibitor 17-Dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG), in hyperthermic conditions. SK-MEL-2 cells were stimulated at 43 °C for 1 h and treated with 0, 0.1 and 1 μM 17-DMAG. We evaluated the cell viability using MTT and apoptosis with HSP 90 inhibitor. We studied the protein expression of AKT, phospho-AKT, ERK, phospho-ERK, MAPK, and phospho-MAPK, caspase 3,7,9, and anti-poly (ADP-ribose) polymerase. 17-DMAG significantly inhibited the proliferation of the SK-MEL-2 cells at 37 °C (0.1 μM: 44.47% and 1 μM: 61.23%) and 43 °C (0.1 μM: 49.21% and 1 μM: 63.60%), suggesting synergism between thermal stimulation and 17-DMAG. 17-DMAG treatment increased the frequency of apoptotic cell populations to 2.17% (0.1 μM) and 3.05% (1 μM) in 37 °C controls, and 4.40% (0.1 μM) and 4.97% (1 μM) in the group stimulated at 43 °C. AKT phosphorylation were activated by thermal stimulation and inhibited by 17-DMAG. Hsp90 inhibitor treatment may be clinically applicable to enhance the apoptosis of melanoma cells in hyperthermic condition. Copyright © 2018 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.

  6. Seasonal dynamics of Pseudocalanus minutus elongatus and Acartia spp. in the southern Baltic Sea (Gdańsk Deep – numerical simulations

    Directory of Open Access Journals (Sweden)

    L. Dzierzbicka-Głowacka

    2006-01-01

    Full Text Available A population dynamics model for copepods is presented, describing the seasonal dynamics of Pseudocalanus minutus elongatus and Acartia spp. in the southern Baltic Sea (Gdańsk Deep. The copepod model was coupled with a one-dimensional physical and biological upper layer model for nutrients (total inorganic nitrogen, phosphate, phytoplankton, microzooplankton, and an early juvenile of herring as a predator. In this model, mesozooplankton (herbivorous copepods has been introduced as an animal having definite patterns of growth in successive stages, reproduction and mortality. The populations are represented by 6 cohorts in different developmental stages, thus assuming that recruitment of the next generation occurs after a fixed period of adult life. The copepod model links trophic processes and population dynamics, and simulates individual growth within cohorts and the changes in biomass between cohorts. The simulations of annual cycles of copepods contain one complete generation of Pseudocalanus and two generations of Acartia in the whole column water, and indicate the importance of growth in the older stages of 6 cohorts of each species, to arrive at a total population biomass. The peaks of copepods' biomass are larger at the turn of June and July for Pseudocalanus and smaller in July for Acartia, lagging that of phytoplankton by ca. two mouths, due to the growth of cohorts in successive stages and egg production by females. The numerical results show that the investigated species could not be the main factor limiting the spring phytoplankton bloom in the Gdańsk Deep, because the initial development was slow for Acartia and faster for Pseudocalanus, but the main development formed after the bloom, in both cases. The phytoplankton bloom is very important in the diet of the adults of the copepods, but it is not particularly important for the youngest part of new generation (early nauplii. However, the simulated microzooplankton biomass was

  7. Facile synthesis of mPEG-luteolin-capped silver nanoparticles with antimicrobial activity and cytotoxicity to neuroblastoma SK-N-SH cells.

    Science.gov (United States)

    Qing, Weixia; Wang, Yong; Li, Xiao; Lu, Minghua; Liu, Xiuhua

    2017-12-01

    We firstly report a facile route for the green synthesis of mPEG-luteolin-capped silver nanoparticles (mPEG-luteolin-AgNPs) using mPEG-luteolin as both the reducer and stabilizer. The reaction was carried out in a stirred aqueous solution at 50°C without additional poisonous reagents. The prepared mPEG-luteolin-AgNPs was characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), zeta potential and UV-vis (UV-vis) spectrum, respectively. The proportions of mPEG-luteolin capped silver nanoparticles is about 89.9%, and the content of silver is 6.65%. The mPEG-luteolin-AgNPs was evaluated the antimicrobial effects on Staphlococcus aureus, Extended spectrum β-Lactamases Staphlococcus aureus, Escherichia Coli and Extended spectrum β-Lactamases Escherichia Coli using drilling hole method. The results showed that both gram-positive and gram-negative bacteria were killed by the mPEG-luteolin-AgNPs at low concentration. Meanwhile, the cell viability assay demonstrated that mPEG-luteolin-AgNPs had toxic effects on human neuroblastoma SK-N-SH cells. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. Characterization of an organic solvent-tolerant thermostable glucoamylase from a halophilic isolate, Halolactibacillus sp. SK71 and its application in raw starch hydrolysis for bioethanol production.

    Science.gov (United States)

    Yu, Hui-Ying; Li, Xin

    2014-01-01

    A halophilic bacterium Halolactibacillus sp. SK71 producing extracellular glucoamylase was isolated from saline soil of Yuncheng Salt Lake, China. Enzyme production was strongly influenced by the salinity of growth medium with maximum in the presence of 5% NaCl. The glucoamylase was purified to homogeneity with a molecular mass of 78.5 kDa. It showed broad substrate specificity and raw starch hydrolyzing activity. Analysis of hydrolysis products from soluble starch by thin-layer chromatography revealed that glucose was the sole end-product, indicating the enzyme was a true glucoamylase. Optimal enzyme activity was found to be at 70°C, pH 8.0, and 7.5% NaCl. In addition, it was highly active and stable over broad ranges of temperature (0-100°C), pH (7.0-12.0), and NaCl concentration (0-20%), showing excellent thermostable, alkali stable, and halotolerant properties. Furthermore, it displayed high stability in the presence of hydrophobic organic solvents. The purified glucoamylase was applied for raw corn starch hydrolysis and subsequent bioethanol production using Saccharomyces cerevisiae. The yield in terms of grams of ethanol produced per gram of sugar consumed was 0.365 g/g, with 71.6% of theoretical yield from raw corn starch. This study demonstrated the feasibility of using enzymes from halophiles for further application in bioenergy production. © 2014 American Institute of Chemical Engineers.

  9. Using clinical symptoms to predict adverse maternal and perinatal outcomes in women with preeclampsia: data from the PIERS (Pre-eclampsia Integrated Estimate of RiSk) study.

    Science.gov (United States)

    Yen, Tin-Wing; Payne, Beth; Qu, Ziguang; Hutcheon, Jennifer A; Lee, Tang; Magee, Laura A; Walters, Barry N; von Dadelszen, Peter

    2011-08-01

    Preeclampsia is a leading cause of maternal morbidity. The clinical challenge lies in predicting which women with preeclampsia will suffer adverse outcomes and would benefit from treatment, while minimizing potentially harmful interventions. Our aim was to determine the ability of maternal symptoms (i.e., severe nausea or vomiting, headache, visual disturbance, right upper quadrant pain or epigastric pain, abdominal pain or vaginal bleeding, and chest pain or dyspnea) to predict adverse maternal or perinatal outcomes. We used data from the PIERS (Pre-eclampsia Integrated Estimate of RiSk) study, a multicentre, prospective cohort study designed to investigate the maternal risks associated with preeclampsia. Relative risks and receiver operating characteristic (ROC) curves were assessed for each preeclampsia symptom and outcome pair. Of 2023 women who underwent assessment, 52% experienced at least one preeclampsia symptom, with 5.2% and 5.3% respectively experiencing an adverse maternal or perinatal outcome. No symptom and outcome pair, in either of the maternal or perinatal groups, achieved an area under the ROC curve value > 0.7, which would be necessary to demonstrate a discriminatory predictive value. Maternal symptoms of preeclampsia are not independently valid predictors of maternal adverse outcome. Caution should be used when making clinical decisions on the basis of symptoms alone in the preeclamptic patient.

  10. Growth, fatty acid profile in major lipid classes and lipid fluidity of Aurantiochytrium mangrovei SK-02 As a function of growth temperature.

    Science.gov (United States)

    Chodchoey, Kanokwan; Verduyn, Cornelis

    2012-01-01

    Aurantiochytrium mangrovei Sk-02 was grown in a medium containing glucose (40 g/l), yeast extract (10 g/L) and sea salts (15 g/L) at temperatures ranging from 12 to 35°C. The fastest growth (µmax= 0.15 h(-1)) and highest fatty acid content of 415 mg/g-dry cell weight were found in the cells grown at 30°C. However, the cells grown at 12°C showed the highest percentage of polyunsaturated fatty acid (PUFA) (48.6% of total fatty acid). The percentage of docosahexaenoic acid (DHA) and pentadecanoic acid (C15:0) decreased with an increase in the growth temperature, whereas, palmitic acid (C16:0), stearic acid (C18:0) and DPA (C22:5n6) increased with an increase in the growth temperature. The composition of the major lipid class (%w/w) was slightly affected by the growth temperature. The fluidity of the organelle membrane or intracellular lipid (by DPH measurement) decreased with an increase in the growth temperatures, while the plasma membrane fluidity (by TMA-DPH measurement) could still maintain its fluidity in a wide range of temperatures (15 - 37°C). Furthermore, the distribution of DHA was found to be higher (36 - 54%) in phospholipid (PL) as compared to neutral lipid (NL) (20 - 41%).

  11. Postavení OPEC v mezinárodních hospodářských vztazích

    OpenAIRE

    Košteková, Eva

    2008-01-01

    Bakalárska práca sa týka Organizácie krajín vyvážajúcich ropu.Cieľom práce je zhodnotiť postavenie OPEC od jeho vzniku, charakterizovať význam tejto organizácie a popísať zapojenie OPEC vo svetovej ekonomike.V prvej časti práce je základná charakteristika OPEC. Druhá časť práce skúma vývoj postavenia OPEC od jeho vzniku v roku 1960 do roku 2000 so zameraním na ropné šoky. Tretia časť práce sa zaoberá postavením OPEC v treťom miléniu, vzťahmi s EÚ a obnoviteľnými zdrojmi energie....

  12. Associations between functional ability and life satisfaction in the oldest old: results from the longitudinal population study Good Aging in Skåne

    Directory of Open Access Journals (Sweden)

    Enkvist Å

    2012-08-01

    Full Text Available Åsa Enkvist, Henrik Ekström, Sölve ElmståhlDepartment of Health Sciences, Lund University, Malmö University Hospital, Malmö, SwedenObjectives: To describe change in functional ability in the oldest-old population during 3 years and examine its relation to life satisfaction (LS. A total of 681 individuals aged 78 and older from the population-based study Good Aging in Skåne took part.Methods: Functional ability was assessed using Sonn and Åsberg's Activities of Daily Living (ADL scale and related to LS assessed by Neugarten et al's Life Satisfaction Index A (LSI-A.Results: Fifty-one percent of 87–93-year-olds reported ADL decline during 3 years. Individuals reporting impaired ADL had a mean LSI-A value of 23.0 compared to 26.4 in those unchanged. ADL decline had a stronger negative effect on LS in the younger group (78–84 years, r = 0.207, P < 0.001. In a multiple regression model, one score's decline in ADL capacity corresponded to 1.5 scores lower LS (P < 0.001.Discussion: Effort put into keeping the oldest old on a high level of functional ability has the potential to maintain the LS of this population.Keywords: life satisfaction, functional ability, longitudinal, oldest old

  13. Performance Analysis of Heterodyne-Detected OCDMA Systems Using PolSK Modulation over a Free-Space Optical Turbulence Channel

    Directory of Open Access Journals (Sweden)

    Fan Bai

    2015-10-01

    Full Text Available This paper presents a novel model of heterodyne-detected optical code-division multiple-access (OCDMA systems employing polarization shift keying (PolSK modulation over a free-space optical (FSO turbulence channel. In this article, a new transceiver configuration and detailed analytical model for the proposed system are provided and discussed, taking into consideration the potential of heterodyne detection on mitigating the impact of turbulence-induced irradiance fluctuation on the performance of the proposed system under the gamma-gamma turbulence channel. Furthermore, we derived the closed-form expressions for the system error probability and outage probability, respectively. We determine the advantages of the proposed modeling by performing a comparison with a direct detection scheme obtained from an evaluation of link performance under the same environment conditions. The presented work also shows the most significant impact factor that degrades the performance of the proposed system and indicates that the proposed approach offers an optimum link performance compared to conventional cases.

  14. Differential gene expression in porcine SK6 cells infected with wild-type and SAP domain-mutant foot-and-mouth disease virus.

    Science.gov (United States)

    Ni, Zixin; Yang, Fan; Cao, Weijun; Zhang, Xiangle; Jin, Ye; Mao, Ruoqing; Du, Xiaoli; Li, Weiwei; Guo, Jianhong; Liu, Xiangtao; Zhu, Zixiang; Zheng, Haixue

    2016-06-01

    Foot-and-mouth disease virus (FMDV) is the causative agent of a highly contagious disease in livestock. The viral proteinase L(pro) of FMDV is involved in pathogenicity, and mutation of the L(pro) SAP domain reduces FMDV pathogenicity in pigs. To determine the gene expression profiles associated with decreased pathogenicity in porcine cells, we performed transcriptome analysis using next-generation sequencing technology and compared differentially expressed genes in SK6 cells infected with FMDV containing L(pro) with either a wild-type or mutated version of the SAP domain. This analysis yielded 1,853 genes that exhibited a ≥ 2-fold change in expression and was validated by real-time quantitative PCR detection of several differentially expressed genes. Many of the differentially expressed genes correlated with antiviral responses corresponded to genes associated with transcription factors, immune regulation, cytokine production, inflammatory response, and apoptosis. Alterations in gene expression profiles may be responsible for the variations in pathogenicity observed between the two FMDV variants. Our results provided genes of interest for the further study of antiviral pathways and pathogenic mechanisms related to FMDV L(pro).

  15. What is the diet of Palaemon elegans Rathke, 1837 (Crustacea, Decapoda, a non-indigenous species in the Gulf of Gdańsk (southern Baltic Sea?

    Directory of Open Access Journals (Sweden)

    Urszula Janas

    2008-06-01

    Full Text Available Palaemon elegans, a new component of the Gulf of Gdańsk macrozoobenthos,colonised the southern Baltic coastal zone in the late 20th and early 21stcentury. Analysis of the stomach contents of P. elegans revealed 16plant and animal taxa that these prawns had fed on. The principal dietarycomponent was detritus, with a mean frequency of occurrence in stomachs of > 80%.The most frequently occurring plant components in the diet were algaefrom the genus Cladophora and the family Ectocarpaceae, while the mostsignificant animal components were Harpacticoida, Chironomidae, Ostracoda andGammarus spp. The results of the study show that the dietary composition ofP. elegans differed significantly between stations and months. The foraging area consisted of two distinctive regions - the Inner Puck Bay, and the Outer Puck Bay together with the Dead Vistula River; two of the stations - Gdynia and Sopot - were distinctfrom all the others. However, no obvious seasonality in the food compositioncould be demonstrated.

  16. Biochanin A induces anticancer effects in SK-Mel-28 human malignant melanoma cells via induction of apoptosis, inhibition of cell invasion and modulation of NF-κB and MAPK signaling pathways.

    Science.gov (United States)

    Xiao, Peng; Zheng, Bowen; Sun, Jiaming; Yang, Jia

    2017-11-01

    The present study aimed to investigate the antitumor activity of Biochanin A in SK-Mel-28 human malignant melanoma cells. An MTT assay was used to study the cytotoxic effects of Biochanin A. In vitro wound healing and invasion assays were used to investigate the effects on cell migration and invasion. Fluorescence microscopy using acridine orange/propidium iodide was used to study effects on cell morphology and apoptosis. Nuclear factor (NF)-κB and mitogen-activated protein kinase (MAPK) protein expression levels were determined by western blot analysis. The results indicated that Biochanin A significantly inhibited the growth of SK-Mel-28 cells in a dose and time dependent manner. Treatment of the cells with Biochanin A induced apoptosis in a dose dependent manner. Additionally, Biochanin A led to inhibition of cell migration and invasion in a dose-dependent manner and upregulated the expression of key proteins in the NF-κB and MAPK signaling pathways.

  17. Protective Effects of Streblus asper Leaf Extract on H2O2-Induced ROS in SK-N-SH Cells and MPTP-Induced Parkinson’s Disease-Like Symptoms in C57BL/6 Mouse

    Directory of Open Access Journals (Sweden)

    Kanathip Singsai

    2015-01-01

    Full Text Available This study investigated the effects of Streblus asper leaf extract (SA on reactive oxygen species (ROS in SK-N-SH cell culture and on motor functions and behaviors in MPTP-treated C57BL/6 mice. SK-N-SH cell viability after incubation with SA for 24 h was measured by MTT assay. Intracellular ROS levels of SK-N-SH cells were quantified after pretreatment with SA (0, 200, 600, and 1000 µg/mL in the presence of H2O2 (300 µM. Male C57BL/6 mice were force-fed with water or 200 mg/kg/day SA for 32 days. Intraperitoneal injection of MPTP was used to induce Parkinson’s disease-like symptoms. Catalepsy, beam balance ability, olfactory discrimination, social recognition, and spontaneous locomotor activity were assessed on days 19, 21, 23, 26, and 32, respectively. In cell culture, SA at 200, 600, and 1000 µg/mL significantly decreased ROS levels in H2O2-treated SK-N-SH cells. MPTP-treated C57BL/6 mice showed a significant change in all parameters tested when compared to the control group. Pretreatment and concurrent treatment with 200 mg/kg/day SA could antagonize the motor and cognitive function deficits induced by MPTP. The results show that SA possesses anti-Parkinson effects in MPTP-treated C57BL/6 mice and that reduction in ROS levels might be one of the mechanisms.

  18. Joint Polish–Finnish sampling of surface waters around the phosphogypsum waste stacks in Gdańsk and Police from 1 to 3 July 2013 – Results of the expedition

    OpenAIRE

    Räike, Antti; Koskela, Jarkko; Knuuttila, Seppo; Lehtoranta, Jouni; Pitkänen, Heikki; Risto, Maarit; Vuorinen, Jyrki

    2015-01-01

    The report describes the results of the joint Polish–Finnish sampling expedition aimed at estimating the possible effects of the two Polish phosphogypsum stacks located in Wislinka (Gdańsk) and Police on the loading of the Baltic Sea and the nearby watercourses. The joint expedition was based on the agreement between the Polish and Finnish Ministers of the Environment in June 2013. The results indicate a clear effect of the phosphogypsum stack on phosphate and total phosphorus concentratio...

  19. Macrovipecetin, a C-type lectin from Macrovipera lebetina venom, inhibits proliferation migration and invasion of SK-MEL-28 human melanoma cells and enhances their sensitivity to cisplatin.

    Science.gov (United States)

    Hammouda, Manel B; Riahi-Chebbi, Ichrak; Souid, Soumaya; Othman, Houcemeddine; Aloui, Zohra; Srairi-Abid, Najet; Karoui, Habib; Gasmi, Ammar; Magnenat, Edith M; Wells, Timothy N C; Clemetson, Kenneth J; Rodríguez-López, José Neptuno; Essafi-Benkhadir, Khadija

    2018-03-01

    The resistance of melanoma cells to cisplatin restricts its clinical use. Therefore, the search for novel tumor inhibitors and effective combination treatments that sensitize tumor cells to this drug are still needed. We purified macrovipecetin, a novel heterodimeric C-type lectin, from Macrovipera lebetina snake venom and investigated its anti-tumoral effect on its own or combined with cisplatin, in human melanoma cells. Biochemical characterization, in vitro cells assays such as viability, apoptosis, adhesion, migration, invasion, Western blotting and in silico analysis were used in this study. Macrovipecetin decreased melanoma cell viability 100 times more than cisplatin. Interestingly, when combined with the drug, macrovipecetin enhanced the sensitivity of SK-MEL-28 cells by augmenting their apoptosis through increased expression of the apoptosis inducing factor (AIF) and activation of ERK 1/2 , p38, AKT and NF-κB. Moreover, macrovipecetin alone or combined with cisplatin induced the expression of TRADD, p53, Bax, Bim and Bad and down-regulated the Bcl-2 expression and ROS levels in SK-MEL-28 cells. Interestingly, these treatments impaired SK-MEL-28 cell adhesion, migration and invasion through modulating the function and expression of αvβ3 integrin along with regulating E-cadherin, vimentin, β-catenin, c-Src and RhoA expression. In silico study suggested that only the α chain of macrovipecetin interacts with a region overlapping the RGD motif binding site on this integrin. We validated the antitumor effect of macrovipecetin when combined, or not, with cisplatin on SK-MEL-28 cells. The presented work proposes the potential use of macrovipecetin and cisplatin in combination as an effective anti-melanoma treatment. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Recombinant rubistatin (r-Rub), an MVD disintegrin, inhibits cell migration and proliferation, and is a strong apoptotic inducer of the human melanoma cell line SK-Mel-28.

    Science.gov (United States)

    Carey, Clayton M; Bueno, Raymund; Gutierrez, Daniel A; Petro, Christopher; Lucena, Sara E; Sanchez, Elda E; Soto, Julio G

    2012-02-01

    Disintegrins are low molecular weight peptides isolated from viper venom. These peptides bind to integrin receptors using a conserved binding motif sequence containing an RGD or similar motif. As a consequence, disintegrins can inhibit platelet aggregation and inhibit cell migration, proliferation, and initiate apoptosis in cancer cell lines. Rubistatin is a MVD disintegrin cloned from a Crotalus ruber ruber venom gland. The biological activity of MVD disintegrins is poorly understood. Recombinant rubistatin (r-Rub) was cloned into a pET32b plasmid and expressed in reductase-deficient Escherichia coli. Expression was induced with IPTG and the resulting fusion peptide was affinity purified, followed by thrombin cleavage, and removal of vector coded sequences. r-Rub peptide inhibited ADP-induced platelet aggregation by 54% ± 6.38 in whole blood. We assessed the ability of r-Rub to initiate apoptosis in three human cancer cell lines. Cultures of SK-Mel-28, HeLA, and T24 cells were grown for 24 h with 2.5 μM r-Rub followed by Hoechst staining. Chromatin fragmentation was observed in treated SK-Mel-28, but not in T24 or HeLA cells. A TUNEL assay revealed that 51.55% ± 5.28 of SK-Mel-28 cells were apoptotic after 18 h of treatment with 3.5 μM of r-Rub. Cell migration and proliferation assays were performed in order to further characterize the biological effects of r-Rub on SK-Mel-28 cells. At 3 μM, r-Rub inhibited cell migration by 44.4% ± 0.5, while at 3.5 μM it was able to inhibit cell proliferation by 83% ± 6.0. Copyright © 2011 Elsevier Ltd. All rights reserved.

  1. [Roman Czaja. Die Anfänge preussischer Hansestädte im Lichte der historischen und archäologischen quellen : Danzig/Gdańsk, Elbing/Elbląg, Thorn/Toruń] / Dennis Hormuth

    Index Scriptorium Estoniae

    Hormuth, Dennis

    2013-01-01

    Arvustus : Roman Czaja. Die Anfänge preussischer Hansestädte im Lichte der historischen und archäologischen quellen : Danzig/Gdańsk, Elbing/Elbląg, Thorn/Toruń. In: Stadtgründung und Stadterweiterung. Beiträge von Archäologie und Stadtgeschichtsforschung. (=Beiträge zur Geschichte der Städte Mitteleuropas XXII). LInz, 2011. S. 59-74

  2. The use of strontium and barium analyses for the reconstruction of the diet of the early medieval coastal population of Gdańsk (Poland): A preliminary study.

    Science.gov (United States)

    Szostek, Krzysztof; Głab, Henryk; Pudło, Aleksandra

    2009-01-01

    Barium and strontium analyses yield an important perspective on temporal shifts in diet in relation to social and environmental circumstances. This research focuses on reconstructing dietary strategies of individuals in the early medieval (12-13th century) population of Gdańsk on the coast of the Baltic Sea. To describe these strategies where seafood rich in minerals was included in the diet, levels of strontium, barium, calcium and phosphorus were measured in first permanent molars of adult men and women whose remains were excavated from the churchyard in the city centre. Faunal remains from the excavation were analysed as an environmental background with respect to the content of the above-mentioned elements. The impact of diagenesis on the odontological material under study was also determined by an analysis of the soil derived from the grave and non-grave surroundings. For verification of diagenetic processes, the calcium/phosphorus index was used. Strontium, calcium, phosphorus and barium levels were determined with the spectrophotometric method using the latest generation plasma spectrophotometer Elan 6100 ICP-MS. From the results of the analysis of taphonomic parameters such as the soil pH, potential ion exchange in the grave surroundings and the Ca/P ratio, it can be inferred that diagenetic factors had little impact on the studied material. From this pilot study we can conclude that in the early Middle Ages in the Baltic Sea basin, seafood was included in the diet from early childhood and at the same time the diet contained calcium-rich milk products (also rich in minerals). The lack of sex differences may indicate the absence of a sex-specific nutritional strategy in childhood and early adolescence.

  3. Atmospheric metal pollution records in the Kovářská Bog (Czech Republic) as an indicator of anthropogenic activities over the last three millennia.

    Science.gov (United States)

    Bohdálková, Leona; Bohdálek, Petr; Břízová, Eva; Pacherová, Petra; Kuběna, Aleš Antonín

    2018-08-15

    Three peat cores were extracted from the Kovářská Bog in the central Ore Mountains to study anthropogenic pollution generated by mining and metallurgy. The core profiles were 14 C dated, and concentrations of selected elements were determined by ICP MS and HG-AAS. Principal component analysis indicated that Pb, Cu, As and Ag may be useful elements for the reconstruction of historical atmospheric pollution. Total and anthropogenic accumulation rates (ARs) of Pb, Cu and As estimated for the last ca. 3500years showed similar chronologies, and revealed twelve periods of elevated ARs of Pb, As and Cu related to possible mining and metallurgic activities. In total, four periods of elevated ARs of Pb, Cu and As were detected during the Middle and Late Bronze Ages, including a distinct Late Bronze Age pollution event between 1030BCE and 910BCE. The Iron Age included three episodes of increased ARs of Pb and As; the first and the most distinctive episode, recorded between 730 and 440BCE, was simultaneous with the Bylany culture during the Hallstatt Period. The Roman Age was characterized by one pollution event, two events were detected in the Middle Ages, and the last two during the modern period. Enhanced element ARs in the late 12th and 15th centuries clearly documented the onset of two periods of intense mining in the Ore Mountains. Metal ARs culminated in ca. 1600CE, and subsequently decreased after the beginning of the Thirty Years' War. The last boom of mining between 1700CE and 1830CE represented the last period of important metallurgical operations. Late Medieval and modern period metal ARs are in good agreement with written documents. Earlier pollution peaks suggest that local metal production could have a much longer tradition than commonly believed; however, archaeological or written evidence is scarce or lacking. Copyright © 2018 Elsevier B.V. All rights reserved.

  4. HLXB9 gene expression, and nuclear location during in vitro neuronal differentiation in the SK-N-BE neuroblastoma cell line.

    Directory of Open Access Journals (Sweden)

    Claudia Giovanna Leotta

    Full Text Available Different parts of the genome occupy specific compartments of the cell nucleus based on the gene content and the transcriptional activity. An example of this is the altered nuclear positioning of the HLXB9 gene in leukaemia cells observed in association with its over-expression. This phenomenon was attributed to the presence of a chromosomal translocation with breakpoint proximal to the HLXB9 gene. Before becoming an interesting gene in cancer biology, HLXB9 was studied as a developmental gene. This homeobox gene is also known as MNX1 (motor neuron and pancreas homeobox 1 and it is relevant for both motor neuronal and pancreatic beta cells development. A spectrum of mutations in this gene are causative of sacral agenesis and more broadly, of what is known as the Currarino Syndrome, a constitutional autosomal dominant disorder. Experimental work on animal models has shown that HLXB9 has an essential role in motor neuronal differentiation. Here we present data to show that, upon treatment with retinoic acid, the HLXB9 gene becomes over-expressed during the early stages of neuronal differentiation and that this corresponds to a reposition of the gene in the nucleus. More precisely, we used the SK-N-BE human neuroblastoma cell line as an in vitro model and we demonstrated a transient transcription of HLXB9 at the 4th and 5th days of differentiation that corresponded to the presence, predominantly in the cell nuclei, of the encoded protein HB9. The nuclear positioning of the HLXB9 gene was monitored at different stages: a peripheral location was noted in the proliferating cells whereas a more internal position was noted during differentiation, that is while HLXB9 was transcriptionally active. Our findings suggest that HLXB9 can be considered a marker of early neuronal differentiation, possibly involving chromatin remodeling pathways.

  5. PRELIMINARY RESULTS OF THE MONUMENTAL TREE MONITORING BASED ON TERRESTRIAL LASER SCANNING - A CASE STUDY OF THE OAK BARTEK IN ZAGNAŃSK (POLAND

    Directory of Open Access Journals (Sweden)

    Wezyk Piotr

    2015-12-01

    Full Text Available In April 2013, the Laboratory of Geomatics launched the project under the acronym “Bartek 3D” in cooperation with the Research Section of Students from the AGH in Krakow, Pedagogical University and the Jagiellonian University as well. The main aim of the project is to monitor the biggest and probably one of the oldest treesin Poland - Oak Bartek in Zagnańsk (N 50o59’14”; E 20o38’59”, based on multi-temporal Terrestrial Laser Scanning (TLS technology. One of the results of the project should be a 3D model ofOak Bartek and detection of the changes in the shape of the tree. Terrestrial Laser Scanning and the traditional forest inventory measurements were performed during the Leaf-OFF season in April 2013 and April 2014 and repeated in Leaf-ON period in July 2013 and October 2014 with using scanners: FARO FOCUS 3D, RIEGL VZ-400, LEICA C10 and RevScan (HandyScan. The results based on TLS technology showed some differences comparing to existing data obtained by traditional measurements for forestry inventory: • Height (H of the tree: altimeter Vertex (Haglöf H = 29.31m; HTLS= 28.49 m; • Trunk circumference (L measured with stretched tape: LST = 9.80 m; adjacent along the shape of bark: LT= 13.70 m; TLS measurments: LTLS1/4 = 9.97 m and LRevScan= 13.54 m • The average diameter at breast height (DBH130cm calculated on the basis of 3D basal area of stem DBHTLS1/4 = 3.03 m (DBHT= 3.12 m.

  6. Neuronal Uptake and Neuroprotective Properties of Curcumin-Loaded Nanoparticles on SK-N-SH Cell Line: Role of Poly(lactide-co-glycolide) Polymeric Matrix Composition.

    Science.gov (United States)

    Djiokeng Paka, Ghislain; Doggui, Sihem; Zaghmi, Ahlem; Safar, Ramia; Dao, Lé; Reisch, Andreas; Klymchenko, Andrey; Roullin, V Gaëlle; Joubert, Olivier; Ramassamy, Charles

    2016-02-01

    Curcumin, a neuroprotective agent with promising therapeutic approach has poor brain bioavailability. Herein, we demonstrate that curcumin-encapsulated poly(lactide-co-glycolide) (PLGA) 50:50 nanoparticles (NPs-Cur 50:50) are able to prevent the phosphorylation of Akt and Tau proteins in SK-N-SH cells induced by H2O2 and display higher anti-inflammatory and antioxidant activities than free curcumin. PLGA can display various physicochemical and degradation characteristics for controlled drug release applications according to the matrix used. We demonstrate that the release of curcumin entrapped into a PLGA 50:50 matrix (NPs-Cur 50:50) is faster than into PLGA 65:35. We have studied the effects of the PLGA matrix on the expression of some key antioxidant- and neuroprotective-related genes such as APOE, APOJ, TRX, GLRX, and REST. NPs-Cur induced the elevation of GLRX and TRX while decreasing APOJ mRNA levels and had no effect on APOE and REST expressions. In the presence of H2O2, both NPs-Cur matrices are more efficient than free curcumin to prevent the induction of these genes. Higher uptake was found with NPs-Cur 50:50 than NPs-Cur 65:35 or free curcumin. By using PLGA nanoparticles loaded with the fluorescent dye Lumogen Red, we demonstrated that PLGA nanoparticles are indeed taken up by neuronal cells. These data highlight the importance of polymer composition in the therapeutic properties of the nanodrug delivery systems. Our study demonstrated that NPs-Cur enhance the action of curcumin on several pathways implicated in the pathophysiology of Alzheimer's disease (AD). Overall, these results suggest that PLGA nanoparticles are a promising strategy for the brain delivery of drugs for the treatment of AD.

  7. Improved outcome of 131I-mIBG treatment through combination with external beam radiotherapy in the SK-N-SH mouse model of neuroblastoma.

    Science.gov (United States)

    Corroyer-Dulmont, Aurélien; Falzone, Nadia; Kersemans, Veerle; Thompson, James; Allen, Danny P; Able, Sarah; Kartsonaki, Christiana; Malcolm, Javian; Kinchesh, Paul; Hill, Mark A; Vojnovic, Boris; Smart, Sean C; Gaze, Mark N; Vallis, Katherine A

    2017-09-01

    To assess the efficacy of different schedules for combining external beam radiotherapy (EBRT) with molecular radiotherapy (MRT) using 131 I-mIBG in the management of neuroblastoma. BALB/c nu/nu mice bearing SK-N-SH neuroblastoma xenografts were assigned to five treatment groups: 131 I-mIBG 24h after EBRT, EBRT 6days after 131 I-mIBG, EBRT alone, 131 I-mIBG alone and control (untreated). A total of 56 mice were assigned to 3 studies. Study 1: Vessel permeability was evaluated using dynamic contrast-enhanced (DCE)-MRI (n=3). Study 2: Tumour uptake of 131 I-mIBG in excised lesions was evaluated by γ-counting and autoradiography (n=28). Study 3: Tumour volume was assessed by longitudinal MR imaging and survival was analysed (n=25). Tumour dosimetry was performed using Monte Carlo simulations of absorbed fractions with the radiation transport code PENELOPE. Given alone, both 131 I-mIBG and EBRT resulted in a seven-day delay in tumour regrowth. Following EBRT, vessel permeability was evaluated by DCE-MRI and showed an increase at 24h post irradiation that correlated with an increase in 131 I-mIBG tumour uptake, absorbed dose and overall survival in the case of combined treatment. Similarly, EBRT administered seven days after MRT to coincide with tumour regrowth, significantly decreased the tumour volume and increased overall survival. This study demonstrates that combining EBRT and MRT has an enhanced therapeutic effect and emphasizes the importance of treatment scheduling according to pathophysiological criteria such as tumour vessel permeability and tumour growth kinetics. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  8. Comparison of the neurotoxicities between volatile organic compounds and fragrant organic compounds on human neuroblastoma SK-N-SH cells and primary cultured rat neurons

    Directory of Open Access Journals (Sweden)

    Yasue Yamada

    2015-01-01

    Full Text Available These are many volatile organic compounds (VOCs that are synthesized, produced from petroleum or derived from natural compounds, mostly plants. Fragrant and volatile organic compounds from plants have been used as food additives, medicines and aromatherapy. Several clinical and pathological studies have shown that chronic abuse of VOCs, mainly toluene, causes several neuropsychiatric disorders. Little is known about the mechanisms of neurotoxicity of the solvents. n-Octanal, nonanal, and 2-ethyl-1-hexanol, which are used catalyzers or intermediates of chemical reactions, are released into the environment. Essential oils have the functions of self-defense, sterilization, and antibiosis in plants. When volatile organic compounds enter the body, there is the possibility that they will pass through the blood–brain barrier (BBB and affect the central nervous system (CNS. However, the direct effects of volatile organic compounds on neural function and their toxicities are still unclear. We compared the toxicities of n-octanal, nonanal and 2-ethyl-1-hexanol with those of five naturally derived fragrant organic compounds (FOCs, linalool, cis-3-hexen-1-ol, isoamyl alcohol, n-propyl alcohol and n-phenethyl alcohol. MTT assay of human neuroblastoma SK-N-SH cells showed that the IC50 values of linalool, cis-3-hexen-1-ol, isoamyl alcohol, n-propyl alcohol and phenethyl alcohol were 1.33, 2.3, >5, >5, and 2.39 mM, respectively, and the IC50 values of toluene, n-octanal, nonanal and 2-ethyl-1-hexanol were 850, 37.2, 8.31 and 15.1 μM, respectively. FOCs showed lower toxicities than those of VOCs. These results indicate that FOCs are safer than other compounds.

  9. Acyclic Cucurbit[n]uril-Type Molecular Container Enables Systemic Delivery of Effective Doses of Albendazole for Treatment of SK-OV-3 Xenograft Tumors.

    Science.gov (United States)

    Hettiarachchi, Gaya; Samanta, Soumen K; Falcinelli, Shane; Zhang, Ben; Moncelet, Damien; Isaacs, Lyle; Briken, Volker

    2016-03-07

    Approximately, 40-70% of active pharmaceutical ingredients (API) are severely limited by their extremely poor aqueous solubility, and consequently, there is a high demand for excipients that can be used to formulate clinically relevant doses of these drug candidates. Here, proof-of-concept studies demonstrate the potential of our recently discovered acyclic cucurbit[n]uril-type molecular container Motor1 (M1) as a solubilizing agent for insoluble drugs. M1 did not induce significant rates of mutations in various Salmonella typhimurium test strains during the Ames test, suggesting low genotoxicity. M1 also has low risk of causing cardiac toxicity in humans since it did not inhibit the human Ether-à-go-go-Related Gene channel as tested on transfected CHO cell lines via patch clamp analysis. Albendazole (ABZ) is a widely used antihelminthic agent but that has also shown promising efficacy against cancerous cells in vitro. However, due to its low aqueous solubility (2.7 μM) and poor pharmacokinetics, ABZ is clinically limited as an anticancer agent. Here we investigated the potential of M1 as a solubilizing excipient for ABZ formulation. A pharmacokinetic study indicated that ABZ escapes the peritoneal cavity resulting in 78% absolute bioavailability, while its active intermediate metabolite, albendazole sulfoxide, achieved 43% absolute bioavailability. The daily dosing of 681 mg/kg M1 complexed with 3.2 mg/kg of ABZ for 14 days did not result in significant weight loss or pathology in Swiss Webster mice. In vivo efficacy studies using this M1·ABZ inclusion complex showed significant decreases in tumor growth rates and increases in survival of mice bearing SK-OV-3 xenograft tumors. In conclusion, we provide substantial new evidence demonstrating that M1 is a safe and efficient excipient that enables in vivo parenteral delivery of poorly water-soluble APIs.

  10. A standardized crisis management model for self-harming and suicidal individuals with three or more diagnostic criteria of borderline personality disorder: The Brief Admission Skåne randomized controlled trial protocol (BASRCT).

    Science.gov (United States)

    Liljedahl, Sophie I; Helleman, Marjolein; Daukantaité, Daiva; Westrin, Åsa; Westling, Sofie

    2017-06-15

    Brief Admission is a crisis and risk management strategy in which self-harming and suicidal individuals with three or more diagnostic criteria of borderline personality disorder self-admit to hospital at times of increasing risk when other efforts to stay safe are failing. Standardized in the current randomized controlled trial, the intensity of Brief Admission Skåne is implemented in durations of three days, with a maximum frequency of three times a month. Brief Admission is integrated into existing treatment plans in advance of crises to prevent reliance on general psychiatric admissions for risk management, as these may be lengthy, unstructured, and of uncertain therapeutic value. The overall objective of the Brief Admission Skåne randomized controlled trial is to determine if Brief Admission can replace general psychiatric admission for self-harming and suicidal individuals with complex mental illness at times of escalating risk. Other objectives of the study are to evaluate whether Brief Admission increases daily functioning and enhances coping, reduces psychiatric symptoms including frequency and severity of self-harm and suicidal behaviours. A final objective is to determine if Brief Admission is an effective crisis management model for this population. Participants are randomized at an individual level to either Brief Admission Skåne plus Treatment as Usual or Treatment As Usual. Based on a priori power analyses, N = 124 participants will be recruited to the study. Data collection is in progress, and will continue until June 2018. All participant data are single-blinded and will be handled with intention-to-treat analysis. Based on the combined clinical experience of our international research group, the Brief Admission Skåne randomized controlled trial upon which the current protocol is based represents the first initiative to standardize, implement and evaluate Brief Admission amongst self-harming and suicidal individuals, including those with

  11. Et skæg

    DEFF Research Database (Denmark)

    Rytter, Mikkel

    2014-01-01

    I islam er profeten Muhammad en refleksion af Guds vilje, og han har følgelig status som “det perfekte menneske’. Profeten er således et ideal og eksempel til efterfølgelse for muslimer verden over. Dette kapitel diskuterer, hvordan danske sufi-muslimer an- lægger sig signifikante fuldskæg – selvom...

  12. The Jurassic of Denmark and Greenland: An offshore transgressive–regressive mudstone-dominated succession from the Sinemurian of Skåne, Sweden

    Directory of Open Access Journals (Sweden)

    Surlyk, Finn

    2003-10-01

    Full Text Available A Sinemurian mudstone-dominated succession was exposed until recently in the Gantofta quarry in Skåne, southern Sweden. The deposits are placed in the Döshult and Pankarp Members of the Sinemurian–Aalenian Rya Formation. Similar facies of the same age are widespread in the Danish Basin where they constitute the F-Ib unit (F-I member of the Fjerritslev Formation. The Gantofta succession thus represents the easternmost extension of the environment characteristic of the Fjerritslev Formation and is essentially the only locality where it has been possible tostudy the facies of this formation in outcrop. Sedimentation seems to have taken place under relatively quiet tectonic conditions except for the possible fault-control of the basin margin. Thelower part of the Gantofta section is of Early and early Late Sinemurian age. It represents the upper part of the Döshult Member and consists of muddy, lower shoreface sandstones, abruptlyoverlain by dark, bioturbated, fossiliferous mudstones with thin storm siltstones and sandstones. They are overlain by the Upper Sinemurian Pankarp Member which comprises red-brown, restricted marine calcareous mudstones with an upwards increasing number of storm siltstones and sandstones reflecting general shallowing and shoreline progradation.The succession spans the greater part of two simple sequences with a distal sequence boundary located at the boundary between the Döshult Member and the Pankarp Member. The exposed part of the lower sequence includes a thick transgressive systems tract and a very thin highstand systems tract. The upper sequence is represented by an undifferentiated transgressive and highstand systems tract. An Early Sinemurian sea-level rise, a late Early Sinemurian highstand, an early Late Sinemurian fall and a Late Sinemurian minor rise and a major fall are recognised. Nearby boreholes show evidence for an end-Sinemurian – Early Pliensbachian major rise. This evolution corresponds well with

  13. PCA and multidimensional visualization techniques united to aid in the bioindication of elements from transplanted Sphagnum palustre moss exposed in the Gdańsk City area.

    Science.gov (United States)

    Astel, Aleksander; Astel, Karolina; Biziuk, Marek

    2008-01-01

    During the last decades, a technique for assessing atmospheric deposition of heavy elements was developed based on the principle that samples of moss are able to accumulate elements and airborne particles from rain, melting snow and dry deposition. Despite a broad interest in bioindication there are still ongoing works aimed at the preparation of a standard procedure allowing for a comparison of research carried out in various areas. This is why the comparison of living and dry moss of the same species and growth site seems to be interesting, logical and promising. A most reliable approach seems to be the application of bioindication connected with multivariate statistics and efficient visualization techniques in the interpretation of monitoring data. The aim of this study was: (i) to present cumulative properties of transplanted Sphagnum palustre moss with differentiation into dry and living biomaterial; (ii) to determine and geographically locate types of pollution sources responsible for a structure of the monitoring data set; (iii) to visualize geographical distribution of analytes in the Gdańsk metropolitan area and to identify the high-risk areas which can be targeted for environmental hazards and public health. A six month air pollution study based on Sphagnum palustre bioindication is presented and a simplified procedure of the experiment is given. The study area was located at the mouth of the Vistula River on the Baltic Sea, in Gdańsk City (Poland). Sphagnum palustre was selected for research because of its extraordinary morphological properties and its ease in being raised. The capability of dry and living moss to accumulate elements characteristic for anthropogenic and natural sources was shown by application of Principal Component Analysis. The high-risk areas and pollution profiles are detected and visualized using surface maps based on Kriging algorithm. The original selection of elements included all those that could be reliably determined by

  14. High-sensitive LC-MS/MS method for the simultaneous determination of mirodenafil and its major metabolite, SK-3541, in human plasma: application to microdose clinical trials of mirodenafil.

    Science.gov (United States)

    Cho, Doo-Yeoun; Bae, Soo Hyeon; Shon, Ji-Hong; Bae, Soo Kyung

    2013-03-01

    A high-sensitivity LC/MS/MS method was developed and validated for the simultaneous determination of mirodenafil and its major metabolite, SK-3541, in human plasma. Mirodenafil, SK-3541, and udenafil as an internal standard were extracted from plasma samples with methyl tert-butyl ether. Chromatographic separation was performed on a Luna phenyl-hexyl column (100 × 2.0 mm) with an isocratic mobile phase consisting of 5 mM ammonium formate and ACN (23:77, v/v) at a flow rate of 0.35 mL/min. Detection and quantification were performed using a mass spectrometer in selected reaction monitoring mode with positive ESI at m/z 532.3 → 296.1 for mirodenafil, m/z 488.1 → 296.1 for SK-3541, and m/z 517.3 → 283.2 for udenafil. The calibration curves were linear over a concentration range of 2-500 pg/mL using 0.5 mL plasma for the microdose of mirodenafil (100 μg). Analytical method validation of the clinical dose (100 mg), with a calibration curve range of 2-500 ng/mL using 0.025-mL plasma, was also conducted. The other LC-MS/MS conditions were similar to those used for the microdosing. Each method was applied successfully to pharmacokinetic studies after a microdose or clinical dose of mirodenafil to six healthy Korean male volunteers. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. The interaction between the light source dose and caspase-dependent and -independent apoptosis in human SK-MEL-3 skin cancer cells following photodynamic therapy with zinc phthalocyanine: A comparative study.

    Science.gov (United States)

    Doustvandi, Mohammad Amin; Mohammadnejad, Fateme; Mansoori, Behzad; Mohammadi, Ali; Navaeipour, Farzaneh; Baradaran, Behzad; Tajalli, Habib

    2017-11-01

    The aim of this study is to determine the behavior of relative expression of Bcl-2, caspase-8, caspase-9, and caspase-3 genes of/in SK-MEL-3 cancer cells and explore molecular mechanisms responsible for the apoptosis response during an in vitro photodynamic therapy (PDT) with Zinc Phthalocyanine (ZnPc) using different doses of the light source. In this study, firstly the cytotoxic effects of ZnPc-PDT on SK-MEL-3 cells were evaluated. By irradiating the laser, ZnPc induced a significant amount of apoptosis on SK-MEL-3 cells in three IC 50 s including 0.064±0.01, 0.043±0.01, and 0.036±0.01μg/mL at the doses of 8, 16, and 24J/cm 2 , respectively. Moreover, flow cytometry and QRT-PCR experiments were done. The high percentage of apoptotic cells was seen in the early apoptosis stage. The expression of Bcl-2 and caspase-8 genes at all doses of laser experienced an obvious reduction in comparison to the control group. On the other hand, although the expression of caspase-9 and caspase-3 genes remains almost constant at 8J/cm 2 , but they faced an increment at 16 and 24J/cm 2 doses. These data reveal caspase-dependent apoptosis in high and caspase-independent apoptosis in low doses of laser. Based on the results of present work, it can be suggested that the dose of the light source is a key factor in induction of caspase-dependent and caspase-independent apoptosis pathways following PDT. Copyright © 2017. Published by Elsevier B.V.

  16. [The Outpatient Clinic for Adolescent Risk-taking and Self-harm behaviors (AtR!Sk) - A Pioneering Approach of Outpatient Early Detection and Intervention of Borderline Personality Disorder].

    Science.gov (United States)

    Kaess, Michael; Ghinea, Denisa; Fischer-Waldschmidt, Gloria; Resch, Franz

    2017-07-01

    The Outpatient Clinic for Adolescent Risk-taking and Self-harm behaviors (AtR!Sk) - A Pioneering Approach of Outpatient Early Detection and Intervention of Borderline Personality Disorder Self-harm and risk-taking behaviors are frequently occurring problems in adolescents' everyday life and commonly challenge the present child and adolescent health-care system. Those behaviors are typical features of borderline-personality disorder (BPD), a severe mental disorder that is associated with immense psychological strain, increased risk of mortality and poor psychosocial functioning. Despite controversy in the past, recent evidence shows that BPD is a valid and reliable diagnosis in adolescence. Consequently, specified and effective intervention programs for this age group are necessary. Instead, present health-care services for children and adolescents in Germany are usually characterized by long waiting periods for specialized outpatient treatments and unnecessary expanses of unnecessary long inpatient stays. Alternatively, and in order to prevent serious long-term consequences, new concepts - integrated in an ambulatory setting and close to patients' daily routines - should focus on early detection and treatment of adolescents at-risk presenting with borderline personality features. The specialist outpatient clinic AtR!Sk at the University Hospital Heidelberg ensures a low-threshold initial contact service, comprehensive and accurate diagnosis of BPD features, and rapidly "tailored" therapy for young people presenting with any types of risk-taking and self-harm behavior. AtR!Sk - as a pioneering approach in south Germany - provides evidence-based early detection and intervention for adolescent BPD.

  17. Mechanizmy regulacji odpowiedzi immunologicznej w zwierzęcym modelu alergicznego kontaktowego zapalenia skóry [Mechanisms involved in the regulation of immune response in animal model of allergic contact dermatitis

    Directory of Open Access Journals (Sweden)

    Anna Strzępa

    2014-09-01

    Full Text Available Streszczenie Alergiczne kontaktowe zapalenie skóry jest klasycznym przykładem późnej reakcji nadwrażliwości typu IV, którą mediują limfocyty efektorowe CD4+ Th1. Rozwój alergicznego kontaktowego zapalenia skóry (ACD jest następstwem ekspozycji na działanie małocząsteczkowych substancji (hapteny. Obecnie ACD jest jedną z najczęstszych chorób zawodowych. Występowanie alergii kontaktowej na co najmniej jeden czynnik w krajach Europy Zachodniej i Ameryki Północnej dochodzi do 19,5% dla różnych grup wiekowych. Klasyczna egzematyczna postać ACD manifestuje się jako rumień, naciek, nadmierne rogowacenie, obrzęk i pęcherze, którym towarzyszy świąd. Modelem zwierzęcym ACD jest reakcja nadwrażliwości kontaktowej (CS. Składa się ona z dwóch następujących po sobie etapów: indukcja (faza aferentna i wywołanie (faza eferentna. W trakcie pierwszego kontaktu z haptenem powstają swoiste limfocyty efektorowe (Tef, które są rekrutowane do miejsca ponownej ekspozycji na hapten. W tym artykule przedstawiono mechanizmy immunologiczne leżące u podstaw ACD, opierając się na modelu CS.

  18. Psychologiczne aspekty chorób alergicznych: trudności dorosłych pacjentów z astmą oskrzelową i atopowym zapaleniem skóry

    Directory of Open Access Journals (Sweden)

    Paulina Albińska

    2014-12-01

    Full Text Available Celem pracy jest zaprezentowanie przeglądu badań na temat psychologicznego funkcjonowania dorosłych cierpiących na choroby alergiczne. Artykuł koncentruje się na dorosłych ze względu na istnienie małej liczby prac naukowych dotyczących zmiany manifestacji symptomów u pacjentów z wyżej wymienionymi chorobami, która może być traktowana jako pierwsze i ostatnie stadium marszu alergicznego, ponieważ jego objawowa manifestacja u dzieci zwykle przybiera postać atopowego zapalenia skóry, podczas gdy u dorosłych jest znana jako astma oskrzelowa. Analiza odpowiada na pytanie, czy wspólnej, alergiczno-zapalnej etiologii tych schorzeń odpowiadają podobieństwa doświadczanych trudności psychologicznych. Omówienie odwołuje się do polskiej i zagranicznej literatury przedmiotu i uwzględnia kluczowe aspekty chorób psychosomatycznych – astmy i atopowego zapalenia skóry – czyli trudności ze snem, depresję, lęk, inne zaburzenia afektywne, zaburzenia zachowania i trudności poznawcze. Współwystępowanie zaburzeń psychicznych i somatycznych u chorych na alergię to niepodważalny dowód integralności somy i psyche, co rzadko dostrzegają osoby odpowiedzialne za system opieki zdrowotnej. Ignorują one tym samym ważny element procesu diagnostyczno-terapeutycznego, istotny dla wyzdrowienia pacjentów z astmą i atopowym zapaleniem skóry.

  19. Functional characterization of six aspartate (D) recombinant mojastin mutants (r-Moj): A second aspartate amino acid carboxyl to the RGD in r-Moj-D_ peptides is not sufficient to induce apoptosis of SK-Mel-28 cells.

    Science.gov (United States)

    Ramos, Carla J; Gutierrez, Daniel A; Aranda, Ana S; Koshlaychuk, Melissa A; Carrillo, David A; Medrano, Rafael; McBride, Terri D; U, Andrew; Medina, Stephanie M; Lombardo, Melissa C; Lucena, Sara E; Sanchez, Elda E; Soto, Julio G

    2016-08-01

    Disintegrins are small peptides produced in viper venom that act as integrin antagonists. When bound to integrins, disintegrins induce altered cellular behaviors, such as apoptotic induction. Disintegrins with RGDDL or RGDDM motifs induce apoptosis of normal and cancer cells. We hypothesized that a second aspartate (D) carboxyl to the RGD is sufficient to induce apoptosis. Five recombinant mojastin D mutants were produced by site-directed mutagenesis (r-Moj-DA, r-Moj-DG, r-Moj-DL, r-Moj-DN, and r-Moj-DV). Stable αv integrin knockdown and shRNA scrambled control SK-Mel-28 cell lines were produced to test a second hypothesis: r-Moj-D_ peptides bind to αv integrin. Only r-Moj-DL, r-Moj-DM, and r-Moj-DN induced apoptosis of SK-Mel-28 cells (at 29.4%, 25.6%, and 36.2%, respectively). Apoptotic induction was significantly reduced in SK-Mel-28 cells with a stable αv integrin knockdown (to 2%, 17%, and 2%, respectively), but not in SK-Mel-28 cells with a stable scrambled shRNA. All six r-Moj-D_ peptides inhibited cell proliferation; ranging from 49.56% (r-Moj-DN) to 75.6% (r-Moj-DA). Cell proliferation inhibition by r-Moj-D_ peptides was significantly reduced in SK-Mel-28 cells with a stable αv integrin knockdown. All six r-Moj-D_ peptides inhibited SK-Mel-28 cell migration at high levels (69%-100%). As a consequence, rac-1 mRNA expression levels were significantly reduced as early as 1 h after treatment, suggesting that rac-1 is involved in the cell migration activity of SK-Mel-28. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Synthesis of carbon-11 labeled 1-(3,4-dimethoxybenzyl)-2,2-dimethyl-1,2,3,4-tetrahydroisoquinolinium derivatives as new potential PET SK{sub Ca} channel imaging agents

    Energy Technology Data Exchange (ETDEWEB)

    Gao Mingzhang; Wang Min [Department of Radiology, Indiana University School of Medicine, 1345 West 16th Street, L-3 Room 202, Indianapolis, IN 46202 (United States); Zheng Qihuang [Department of Radiology, Indiana University School of Medicine, 1345 West 16th Street, L-3 Room 202, Indianapolis, IN 46202 (United States)], E-mail: qzheng@iupui.edu

    2008-02-15

    Small conductance Ca{sup 2+}-activated K{sup +} (SK{sub Ca}) channels play an important role in many functions such as neuronal communication and behavioral plasticity, secretion, and cell proliferation. SK{sub Ca} channel modulation is associated with various brain, heart, and cancer diseases. N-methyl-laudanosine and its structurally related derivatives, substituted 1-(3,4-dimethoxybenzyl)-2,2-dimethyl-1,2,3,4-tetrahydroisoquinoliniums, are reversible and selective SK{sub Ca} channel blockers. Carbon-11 labeled N-methyl-laudanosine and its tetrahydroisoquinolinium derivatives may serve as new probes for positron emission tomography (PET) to image SK{sub Ca} channels in the brain, heart, and cancer. The key intermediates, substituted isoquinolines (3a-c), were synthesized using a modification of the Pomeranz-Fritsch procedure. The precursors, substituted 1-(3,4-dimethoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinolines (8a-c), and their corresponding reference standards, substituted 1-(3,4-dimethoxybenzyl)-2,2-dimethyl-1,2,3,4-tetrahydroisoquinoliniums (9a-c), were synthesized from compounds 3a-c with 3,4-dimethoxybenzyl chloride (2) in multiple steps with moderate to excellent chemical yields. The precursor 6,7-dimethoxy-1-(3,4-dimethoxybenzyl)-2-methyl-1,2,3, 4-tetrahydroisoquinoline (10) was commercially available, and the methylation of compound 10 with methyl iodide provided N-methyl-laudanosine (11). The target quaternary ammonium tracers, carbon-11 labeled 1-(3,4-dimethoxybenzyl)-2,2-dimethyl-1,2,3,4-tetrahydroisoquinoliniums ([{sup 11}C]9a-c and [{sup 11}C]11), were prepared by N-[{sup 11}C]methylation of the tertiary amine precursors (8a-c and 10) with [{sup 11}C]methyl triflate and isolated by a simplified solid-phase extraction (SPE) purification using a SiO{sub 2} or cation-exchange CM Sep-Pak cartridge in 40-65% radiochemical yields.

  1. Rola pielęgniarki w opiece nad pacjentem z atopowym zapaleniem skóry = Nurse’s role in taking care of a patient with atopic dermatitis

    Directory of Open Access Journals (Sweden)

    Karolina Filipska

    2016-01-01

    3Wydział Kultury Fizycznej, Zdrowia i Turystyki, Uniwersytet Kazimierza Wielkiego w Bydgoszczy   Słowa kluczowe: atopowe zapalenie skóry, psychologiczne aspekty chorób, świąd, rola pielęgniarki. Keywords: atopic dermatitis, psychological aspects of diseases, itching, nurse’s role.   Streszczenie Wstęp. Atopowe zapalenie skóry (AZS, ang. atopic dermatitis jest chorobą alergiczną o przewlekłym i nawracającym przebiegu. Toczący się proces chorobowy dotyczy naskórka i skóry właściwej. AZS to najpowszechniej występująca przewlekła choroba zapalna dzieci, której patogeneza nie jest do końca poznana. Uznaje się, że na rozwój choroby składają się czynniki genetyczne, immunologiczne, oraz środowiskowe. Cel. Przedstawienie roli pielęgniarki w opiece nad pacjentem z atopowym zapaleniem skóry. Materiał i metody. Dokonano przeglądu 43 doniesień naukowych – artykuły badawcze i poglądowe oraz literatura współczesna - obejmujących swą tematyką rolę pielęgniarki w opiece nad pacjentem z atopowym zapaleniem skóry. Posługując się słowami kluczowymi: atopowe zapalenie skóry, psychologiczne aspekty chorób, świąd rola pielęgniarki przeszukano bazy bibliograficzne  Ebsco, ScienceDirect, SpringerLink, Medline. Wyniki. Zadania pielęgnacyjno-opiekuńcze wynikają z poszczególnych symptomów AZS. Wśród założeń opieki pielęgniarskiej wymienia się: zmniejszenie świądu i suchości skóry;  zminimalizowanie zaburzeń sennych; obniżenie ryzyka wtórych nadkażeń bakteryjnych, wirusowych lub grzybiczych; oraz kompleksowa edukacja chorego i jego rodziny w celu szybszej rekonwalescencji i wydłużenia okresów remisji. Wnioski. AZS wpływa negatywnie na domenę fizyczną, psychiczną i społeczną chorego. Ze względu na swoją przewlekłość oraz szerokie roz­powszechnienie należy do jednego z największych wyzwań zarówno dla pielęgniarki jak i całego zespołu terapeutycznego. Skuteczne leczenie dermatologiczne, w

  2. TRAIL Activates a Caspase 9/7-Dependent Pathway in Caspase 8/10-Defective SK-N-SH Neuroblastoma Cells with Two Functional End Points: Induction of Apoptosis and PGE2 Release

    Directory of Open Access Journals (Sweden)

    Giorgio Zauli

    2003-09-01

    Full Text Available Most neuroblastoma cell lines do not express apical caspases 8 and 10, which play a key role in mediating tumor necrosis factor-related apoptosis-inducing ligand (TRAIL cytotoxicity in a variety of malignant cell types. In this study, we demonstrated that TRAIL induced a moderate but significant increase of apoptosis in the caspase 8/10-deficient SK-N-SH neuroblastoma cell line, through activation of a novel caspase 9/7 pathway. Concomitant to the induction of apoptosis, TRAIL also promoted a significant increase of prostaglandin E2 (PGE2 release by SKN-SH cells. Moreover, coadministration of TRAIL plus indomethacin, a pharmacological inhibitor of cyclooxygenase (COX, showed an additive effect on SKN-SH cell death. In spite of the ability of TRAIL to promote the phosphorylation of both ERKi/2 and p38/MAPK, which have been involved in the control of COX expression/activity, neither PD98059 nor SB203580, pharmacological inhibitors of the ERKi/2 and p38/MAPK pathways, respectively, affected either PGE2 production or apoptosis induced by TRAIL. Finally, both induction of apoptosis and PGE2 release were completely abrogated by the broad caspase inhibitor z-VAD4mk, suggesting that both biologic end points were regulated in SK-N-SH cells through a caspase 9/7-dependent pathway.

  3. The opposing effects of calmodulin, adenosine 5 prime -triphosphate, and pertussis toxin on phorbol ester induced inhibition of atrial natriuretic factor stimulated guanylate cyclase in SK-NEP-1 cells

    Energy Technology Data Exchange (ETDEWEB)

    Sekiya, M.; Frohlich, E.D.; Cole, F.E. (Alton Ochsner Medical Foundation, New Orleans, LA (USA))

    1991-01-01

    In the present study, we investigated the effects of calmodulin, adenosine 5{prime}-triphosphate (ATP) and pertussis toxin (PT) on phorbol ester (PMA) induced inhibition of ANF-stimulated cyclic GMP formation in cells from the human renal cell line, SK-NEP-1. PMA inhibited ANF-stimulated guanylate cyclase activity in particulate membranes by about 65%. Calmodulin reversed this inhibition in a dose dependent manner. ATP potentiated Mg++ but not Mn++ supported guanylate cyclase activity. In PMA treated membranes, ATP potentiating effects were abolished. PMA also inhibited ANF-stimulated cGMP accumulation, but pretreatment with PT prevented this PMA inhibition. PT did not affect basal or ANF-stimulated cGMP accumulation. In conclusion, these results demonstrated that PMA inhibited ANF stimulation of particulate guanylate cyclase in opposition to the activating effects of calmodulin or ATP in SK-NEP-1 cells. The protein kinase C inhibitory effects appeared to be mediated via a PT-sensitive G protein.

  4. Niewirusowy transfer genów do komórek skóry – wybrane metody = Non-viral gene transfer into skin cells – selected methods

    Directory of Open Access Journals (Sweden)

    Ewelina Wędrowska

    2016-01-01

    3Department of Pathobiochemistry and Clinical Chemistry, Nicolaus Copernicus University Collegium Medicum, Bydgoszcz, Poland       Streszczenie   Wprowadzenie: Skóra jako największy i najłatwiej dostępny narząd stanowi atrakcyjny cel dla terapii genowej, która od wielu lat budzi ogromne nadzieje środowiska naukowego. Jednakże, próby terapii przeprowadzone z wykorzystaniem wektorów wirusowych wykazały szereg wad i ograniczeń m.in. obserwowano indukcję odpowiedzi immunologicznej, losową integrację transgenu z genomem gospodarza i/lub niską wydajność jego ekspresji. Dlatego, wciąż poszukuje się alternatywnych, skuteczniejszych i jednocześnie bezpieczniejszych metod transferu genów. Atrakcyjnej alternatywy upatruje się w metodach niewirusowych. Cel pracy: Przedstawienie wybranych metod niewirusowego transferu genów wykorzystywanych w terapii genowej chorób skóry. Skrócony opis stanu wiedzy: Terapia genowa chorób skóry obejmuje wykorzystanie wektorów plazmidowych jako nośnika genów terapeutycznych, a także metod ich dostarczania do komórek takich jak: elektroporacja, mikroiniekcja, sonikacja, wykorzystanie nośników lipidowych i polimerów kationowych. Podsumowanie: Niewirusowe metody transferu genów oferują pewne zalety włączając niską toksyczność, brak infekcyjności oraz łatwość i niskie koszty produkcji w porównaniu z technikami wirusowymi. Niewirusowe metody wydają się być obiecującym narzędziem terapii genowej chorób skóry w szczególności nowotworów tego narządu.   Słowa kluczowe: plazmid, transfer genów, skóra, wirus, terapia genowa.     Summary Introduction: Skin, the largest and most accessible organ of the human body is considered as an ideal gene therapy target. However, various types of viral vectors used in classical gene therapy have a number of disadvantages, such as possibility of immune response induction, random integration of inserts into the host genome or low expression efficiency

  5. Survivin knockdown increased anti-cancer effects of (−)-epigallocatechin-3-gallate in human malignant neuroblastoma SK-N- BE2 and SH-SY5Y cells

    Science.gov (United States)

    Hossain, Md. Motarab; Banik, Naren L.; Ray, Swapan K.

    2012-01-01

    Neuroblastoma is a solid tumor that mostly occurs in children. Malignant neuroblastomas have poor prognosis because conventional chemotherapeutic agents are hardly effective. Survivin, which is highly expressed in some malignant neuroblastomas, plays a significant role in inhibiting differentiation and apoptosis and promoting cell proliferation, invasion, and angiogenesis. We examined consequences of survivin knockdown by survivin short hairpin RNA (shRNA) plasmid and then treatment with (−)-epigallocatechin-3-gallate (EGCG), a green tea flavonoid, in malignant neuroblastoma cells. Our Western blotting and laser scanning confocal immunofluorescence microscopy showed that survivin was highly expressed in malignant neuroblastoma SK-N-BE2 and SH-SY5Y cell lines and slightly in SK-N-DZ cell line. Expression of survivin was very faint in malignant neuroblastoma IMR32 cell line. We transfected SK-N-BE2 and SH-SY-5Y cells with survivin shRNA, treated with EGCG, and confirmed knockdown of survivin at mRNA and protein levels. Survivin knockdown induced morphological features of neuronal differentiation, as we observed following in situ methylene blue staining. Combination of survivin shRNA and EGCG promoted neuronal differentiation biochemically by increases in expression of NFP, NSE, and e-cadherin and also decreases in expression of Notch-1, ID2, hTERT, and PCNA. Our in situ Wright staining and Annexin V-FITC/PI staining showed that combination therapy was highly effective in inducing, respectively, morphological and biochemical features of apoptosis. Apoptosis occurred with activation of caspase-8 and cleavage of Bid to tBid, increase in Bax:Bcl-2 ratio, mitochondrial release of cytochrome c, and increases in expression and activity of calpain and caspase-3. Combination therapy decreased migration of cells through matrigel and inhibited proliferative (p-Akt and NF-κB), invasive (MMP-2 and MMP-9), and angiogenic (VEGF and b-FGF) factors. Also, in vitro network

  6. Survivin knockdown increased anti-cancer effects of (−)-epigallocatechin-3-gallate in human malignant neuroblastoma SK-N-BE2 and SH-SY5Y cells

    International Nuclear Information System (INIS)

    Hossain, Md. Motarab; Banik, Naren L.; Ray, Swapan K.

    2012-01-01

    Neuroblastoma is a solid tumor that mostly occurs in children. Malignant neuroblastomas have poor prognosis because conventional chemotherapeutic agents are hardly effective. Survivin, which is highly expressed in some malignant neuroblastomas, plays a significant role in inhibiting differentiation and apoptosis and promoting cell proliferation, invasion, and angiogenesis. We examined consequences of survivin knockdown by survivin short hairpin RNA (shRNA) plasmid and then treatment with (−)-epigallocatechin-3-gallate (EGCG), a green tea flavonoid, in malignant neuroblastoma cells. Our Western blotting and laser scanning confocal immunofluorescence microscopy showed that survivin was highly expressed in malignant neuroblastoma SK-N-BE2 and SH-SY5Y cell lines and slightly in SK-N-DZ cell line. Expression of survivin was very faint in malignant neuroblastoma IMR32 cell line. We transfected SK-N-BE2 and SH-SY-5Y cells with survivin shRNA, treated with EGCG, and confirmed knockdown of survivin at mRNA and protein levels. Survivin knockdown induced morphological features of neuronal differentiation, as we observed following in situ methylene blue staining. Combination of survivin shRNA and EGCG promoted neuronal differentiation biochemically by increases in the expression of NFP, NSE, and e-cadherin and also decreases in the expression of Notch-1, ID2, hTERT, and PCNA. Our in situ Wright staining and Annexin V-FITC/PI staining showed that combination therapy was highly effective in inducing, respectively, morphological and biochemical features of apoptosis. Apoptosis occurred with activation of caspase-8 and cleavage of Bid to tBid, increase in Bax:Bcl-2 ratio, mitochondrial release of cytochrome c, and increases in the expression and activity of calpain and caspase-3. Combination therapy decreased migration of cells through matrigel and inhibited proliferative (p-Akt and NF-κB), invasive (MMP-2 and MMP-9), and angiogenic (VEGF and b-FGF) factors. Also, in vitro

  7. Wpływ regularnej aktywności fizycznej na skład ciała i ciśnienie tętnicze dzieci ze szkoły sportowej

    Directory of Open Access Journals (Sweden)

    Małgorzata Stańczyk

    2013-06-01

    Full Text Available Wstęp: Trening fizyczny uważany jest za czynnik wpływający na parametry antropometryczne i ciśnienie tęt‑ nicze. Twierdzi się, że osoby regularnie ćwiczące mają lepsze wskaźniki składu ciała oraz niższe wartości ciśnienia tętniczego krwi w porównaniu z osobami nieuprawiającymi sportu. Cel: Ocena wpływu regularnej rocznej aktywności fizycznej prowadzonej w szkole sportowej na skład ciała oraz wartości ciśnienia tętnicze‑ go u dzieci 14-letnich (II klasa gimnazjum. Materiał i metodyka: W badaniu wzięło udział 25 dzieci z gim‑ nazjum sportowego. Dzieci badano dwukrotnie w odstępie 12 miesięcy. Analizie poddano zmianę parame‑ trów antropometrycznych, składu ciała metodą impedancji bioelektrycznej oraz wartości ciśnienia krwi. Wyniki: W całej grupie zaobserwowano wzrost wartości centylowych ciśnienia skurczowego krwi (p < 0,05, przede wszystkim u chłopców. W zakresie ciśnienia rozkurczowego nie zanotowano istotnych różnic. Skład ciała zmienił się niekorzystnie u wszystkich badanych – wzrósł udział tłuszczu (13% vs 20%, p < 0,05, a przy‑ rost masy tłuszczowej był wyższy u dziewczynek (6% vs 3%, p < 0,05. Centyl BMI istotnie statystycznie wzrósł w grupie dziewczynek (37 vs 49, p < 0,05, u chłopców nie uległ zmianie. Wnioski: Aktywność fizyczna proponowana przez szkołę sportową nie poprawia stanu odżywienia 13–14-letnich dziewcząt i chłopców, u których obserwuje się istotne zwiększenie w organizmie zawartości tkanki tłuszczowej. Szkolna aktywność fizyczna nie powoduje obniżenia ciśnienia tętniczego wśród młodzieży w wieku 13–14 lat w odniesieniu do norm wiekowych. W zakresie ciśnienia skurczowego w grupie chłopców zaobserwowano nawet wyższe wartości centylowe.

  8. Accumulation of 24S-hydroxycholesterol in neuronal SK-N-BE cells treated with hexacosanoic acid (C26:0): argument in favor of 24S-hydroxycholesterol as a potential biomarker of neurolipotoxicity.

    Science.gov (United States)

    Zarrouk, A; Hammami, M; Moreau, T; Lizard, G

    2015-02-01

    Cholesterol oxide derivatives (oxysterols) are viewed as potential biomarkers of neurodegenerative diseases. 24S-hydroxycholesterol, an oxysterol produced only in brain neurons, is often found for unknown reasons in increased levels in the plasma in patients with neurodegenerative diseases. On human neuronal SK-N-BE cells treated with hexacosanoic acid (C26:0) identified at increased levels in the tissues and plasma of patients with peroxisomal leukodystrophies and Alzheimer's disease, we observed increased level of 24S-hydroxycholesterol associated with C26:0 induced lipotoxicity. This finding reinforces the hypothesis suggesting that 24S-hydroxycholesterol could constitute a biomarker of neurotoxicity. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  9. Some recollections on acousto-optics research at the University of Gdańsk in the light of international cooperation and of the history of Spring Schools on Acousto-Optics and Applications.

    Science.gov (United States)

    Kwiek, Piotr; Sliwiński, Antoni

    2009-03-01

    Some historical features of international cooperation that have been a background for originating the idea to organize since 1980 special international meetings named Spring Schools on Acousto-Optics and Applications and for continuing the events until now are presented. Thanks to the establishment of the Spring Schools, the group of acousto-opticians at the Gdańsk University have had the opportunity to exchange scientific experience and continue mutual cooperation in research with several acousto-optic centers and to publish a number of common papers. A few examples from these achievements and some historical facts illustrating research activity in the field of acousto-optics during past 30 years are recollected.

  10. Expression, purification, and analysis of three recombinant ECD disintegrins (r-colombistatins) from P-III class snake venom metalloproteinases affecting platelet aggregation and SK-MEL-28 cell adhesion.

    Science.gov (United States)

    Suntravat, Montamas; Helmke, Thomas J; Atphaisit, Chairat; Cuevas, Esteban; Lucena, Sara E; Uzcátegui, Nestor L; Sánchez, Elda E; Rodriguez-Acosta, Alexis

    2016-11-01

    Crotalid venoms are rich sources of components that affect the hemostatic system. Snake venom metalloproteinases are zinc-dependent enzymes responsible for hemorrhage that also interfere with hemostasis. The disintegrin domain is a part of snake venom metalloproteinases, which involves the binding of integrin receptors. Integrins play an essential role in cancer survival and invasion, and they have been major targets for drug development and design. Both native and recombinant disintegrins have been widely investigated for their anti-cancer activities in biological systems as well as in vitro and in vivo systems. Here, three new cDNAs encoding ECD disintegrin-like domains of metalloproteinase precursor sequences obtained from a Venezuelan mapanare (Bothrops colombiensis) venom gland cDNA library have been cloned. Three different N- and C-terminal truncated ECD disintegrin-like domains of metalloproteinases named colombistatins 2, 3, and 4 were amplified by PCR, cloned into a pGEX-4T-1 vector, expressed in Escherichia coli BL21, and tested for inhibition of platelet aggregation and inhibition of adhesion of human skin melanoma (SK-Mel-28) cancer cell lines on collagen I. Purified recombinant colombistatins 2, 3, and 4 were able to inhibit ristocetin- and collagen-induced platelet aggregation. r-Colombistatins 2 showed the most potent inhibiting SK-Mel-28 cancer cells adhesion to collagen. These results suggest that colombistatins may have utility in the development of therapeutic tools in the treatment of melanoma cancers and also thrombotic diseases. Copyright © 2016. Published by Elsevier Ltd.

  11. Jón Ólafsson (ed., Lýðræðistilraunir. Ísland í hruni og endurreisn [Democratic experiments. Iceland in collapse and renaissance] (Reykjavík: Háskólaútgáfan, 2014

    Directory of Open Access Journals (Sweden)

    Guðmundur Heiðar Frímannsson

    2015-03-01

    Full Text Available Review of the following book: Jón Ólafsson (ed., Lýðræðistilraunir. Ísland í hruni og endurreisn [Democratic experiments. Iceland in collapse and renaissance], (Reykjavík: Háskólaútgáfan, 2014

  12. Skłonność do unikania i uchylania się od opodatkowania w świetle teorii moralnego rozwoju Kohlberga – zagadnienia etyczne w praktyce rachunkowości

    Directory of Open Access Journals (Sweden)

    Sabina Kołodziej

    2016-04-01

    Full Text Available Współczesne służby rachunkowości mają w swoich rękach narzędzia nie tylko umożliwiające ewidencjonowanie operacji gospodarczych w księgach rachunkowych, ale również mogące służyć zarówno do zniekształcania obrazu sytuacji finansowej podmiotu, jak i manipulowania rozliczeniami podatkowymi. Dlatego profesjonaliści w rachunkowości muszą posiąść określoną wiedzę, ale także powinni stać na straży standardów etycznych, co gwarantuje prawidłowość rozliczeń podatkowych oraz sporządzanie informacji wiarygodnej w systemie informacyjnym rachunkowości. Celem artykułu jest przedstawienie wyników badań analizujących częstość wyboru przez księgowych działań zmierzających do redukcji obciążenia podatkowego, będących skutkiem złamania norm etycznych lub prawnych. Ponadto analizowano związek skłonności do podjęcia decyzji prowadzącej do unikania lub uchylania się od opodatkowania z przedstawioną zgodnie z teorią rozwoju moralnego Kohlberga argumentacją takich działań. Wyniki badań wskazują na większą częstość decyzji obniżających zobowiązania podatkowe w wyniku złamania normy etycznej niż etycznej i prawnej. Stwierdzono również, że skłonność do podejmowania działań nieetycznych (i niezgodnych z prawem jest związana z niższymi poziomami wnioskowania moralnego, co potwierdza ważność postulatu uzupełnienia edukacji księgowych o zagadnienia świadomości i odpowiedzialności etycznej przedstawicieli tego zawodu.

  13. Both deterioration and improvement in activities of daily living are related to falls: a 6-year follow-up of the general elderly population study Good Aging in Skåne

    Directory of Open Access Journals (Sweden)

    Stenhagen M

    2014-10-01

    Full Text Available Magnus Stenhagen, Henrik Ekström, Eva Nordell, Sölve Elmståhl Department of Health Sciences, Division of Geriatric Medicine, Lund University, Skåne University Hospital, Malmö, Sweden Objectives: To determine the relationship between long-term change in activities of daily living (ADL and falls in the elderly and to identify characteristics of groups at risk for falls.Methods: This was a 6-year, prospective cohort study using data from the Good Aging in Skåne study in southern Sweden, involving 1,540 elderly subjects, including the oldest-old (age, 60–93 years. The subjects were recruited from the general population. ADL was measured at a baseline and follow-up assessment, using Sonn and Åsberg’s revised scale and the ADL staircase. Falls were recorded in a period of 6 months before the follow-up assessment. The association between falls and change in ADL was calculated using adjusted, multiple logistic regression analysis and presented in odds ratios (ORs.Results: Thirteen percent of the study population reported one or several falls in the measured period. Over the course of 6 years, one in four participants changed their ADL status, and parts of this category had an increased risk for falls compared with those who stayed independent in ADL or who had no change in the ADL staircase. Groups with different characteristics had a prominent risk for falls: those with a reduction of two to eight steps in the ADL staircase (OR, 4.05; 95% confidence interval [CI], 1.62–10.11 and those becoming independent from dependency in instrumental ADL (OR, 4.13; 95% CI, 1.89–9.00. The former group had advanced age with a greater burden of cognitive impairment, gait disability, arrhythmia, and fall risk medications. The latter group had a higher prevalence of ischemic heart disease and low walking speed. Conclusion: Both deterioration and improvement in ADL over the course of 6 years increased the risk for falls in a general elderly population

  14. Neuroprotective effect of CNB-001, a novel pyrazole derivative of curcumin on biochemical and apoptotic markers against rotenone-induced SK-N-SH cellular model of Parkinson's disease.

    Science.gov (United States)

    Jayaraj, Richard L; Tamilselvam, Kuppusamy; Manivasagam, Thamilarasan; Elangovan, Namasivayam

    2013-11-01

    Oxidative stress and mitochondrial dysfunction are underpinned for initiating a cascade of toxic events leading to dopaminergic neuronal death in Parkinson's disease (PD) and identified as vital target for therapeutic intervention. Curcumin, a potent antioxidant has been reported to display diverse neuroprotective properties against various neurodegenerative diseases including PD. In this present study, we investigated the protective effect of CNB-001, a pyrazole derivative of curcumin on rotenone-induced toxicity and its possible mechanisms in neuroblastoma SK-N-SH cells. Rotenone insult significantly reduced cell viability (MTT assay) and resulted in 78 % apoptosis (dual staining) by altering Bcl-2, Bax, caspase-3, and cytochrome C expression. Moreover, rotenone enhanced ROS production and disrupts mitochondrial membrane potential. These resultant phenotypes were distinctly alleviated by CNB-001. Pretreatment with CNB-001(2 μM) 2 h before rotenone exposure (100 nM) increased cell viability, decreased ROS formation, maintained normal physiological mitochondrial membrane potential, and reduced apoptosis. Furthermore, CNB-001 inhibited downstream apoptotic cascade by increasing the expression of vital antiapoptotic protein Bcl-2 and decreased the expression of Bax, caspase-3, and cytochrome C. Collectively, the results suggest that CNB-001 protects neuronal cell against toxicity through antioxidant and antiapoptotic properties through its action on mitochondria. Therefore, it may be concluded that CNB-001 can be further developed as a promising drug for treatment of PD.

  15. Induction of Mitochondrial Changes Associated with Oxidative Stress on Very Long Chain Fatty Acids (C22:0, C24:0, or C26:0-Treated Human Neuronal Cells (SK-NB-E

    Directory of Open Access Journals (Sweden)

    Amira Zarrouk

    2012-01-01

    Full Text Available In Alzheimer's disease, lipid alterations point towards peroxisomal dysfunctions. Indeed, a cortical accumulation of saturated very long chain fatty acids (VLCFAs: C22:0, C24:0, C26:0, substrates for peroxisomal β-oxidation, has been found in Alzheimer patients. This study was realized to investigate the effects of VLCFAs at the mitochondrial level since mitochondrial dysfunctions play crucial roles in neurodegeneration. On human neuronal SK-NB-E cells treated with C22:0, C24:0, or C26:0 (0.1–20 μM; 48 h, an inhibition of cell growth and mitochondrial dysfunctions were observed by cell counting with trypan blue, MTT assay, and measurement of mitochondrial transmembrane potential (Δψm with DiOC6(3. A stimulation of oxidative stress was observed with DHE and MitoSOX used to quantify superoxide anion production on whole cells and at the mitochondrial level, respectively. With C24:0 and C26:0, by Western blotting, lower levels of mitochondrial complexes III and IV were detected. After staining with MitoTracker and by transmission electron microscopy used to study mitochondrial topography, mass and morphology, major changes were detected in VLCFAs treated-cells: modification of the cytoplasmic distribution of mitochondria, presence of large mitochondria, enhancement of the mitochondrial mass. Thus, VLCFAs can be potential risk factors contributing to neurodegeneration by inducing neuronal damages via mitochondrial dysfunctions.

  16. Individual- and Family-Level Determinants of Risky Sexual Behavior Among Swedish- and Foreign-Born Young Adults 18-30 Years of Age, Residing in Skåne, Sweden.

    Science.gov (United States)

    Asamoah, Benedict Oppong; Agardh, Anette

    2018-02-01

    In Sweden, various public health interventions have been performed to reduce risky sexual behaviors among young people and promote safer and positive approaches to sexuality, while attempting to bridge the gap between the less privileged or more vulnerable young people and their more privileged peers. This study aimed to compare the individual- and familial-level determinants of risky sexual behavior among foreign-born and Swedish-born young adults 18-30 years of age residing in Skåne, the south of Sweden. This was a cross-sectional study that used a questionnaire to collect data from 2968 randomly selected respondents between 18 and 30 years between January and March 2013. The associations were analyzed using chi-square tests, and simple and multiple logistic regression analyses. Younger age, i.e., individual-level factor, and living with only one parent or another person while growing up, i.e., familial-level factor, increased the risk of engaging in sexual risk taking for both Swedish- and foreign-born youth. Male gender was related to a higher risk of engaging in sexual risk-taking behaviors among foreign-born youth but was not as important as influence on sexual risk taking among Swedish-born youth. Parental education level, on the other hand, was significantly associated with sexual intercourse on the "first night" and early sexual debut solely among Swedish-born youth. Condom use was not associated with any family-level factor among both Swedish-born and foreign-born youth. The design of sexual reproductive health and rights messages and interventions to target risky sexual behavior among Swedish youth should take into consideration immigration status (for example, being Swedish-born or foreign-born), individual- and family-level characteristics, as well as the type of behavioral change or outcome desired.

  17. Effects of cholesterol oxides on cell death induction and calcium increase in human neuronal cells (SK-N-BE) and evaluation of the protective effects of docosahexaenoic acid (DHA; C22:6 n-3).

    Science.gov (United States)

    Zarrouk, Amira; Nury, Thomas; Samadi, Mohammad; O'Callaghan, Yvonne; Hammami, Mohamed; O'Brien, Nora M; Lizard, Gérard; Mackrill, John J

    2015-07-01

    Some oxysterols are associated with neurodegenerative diseases. Their lipotoxicity is characterized by an oxidative stress and induction of apoptosis. To evaluate the capacity of these molecules to trigger cellular modifications involved in neurodegeneration, human neuronal cells SK-N-BE were treated with 7-ketocholesterol, 7α- and 7β-hydroxycholesterol, 6α- and 6β-hydroxycholesterol, 4α- and 4β-hydroxycholesterol, 24(S)-hydroxycholesterol and 27-hydroxycholesterol (50-100μM, 24h) without or with docosahexaenoic acid (50μM). The effects of these compounds on mitochondrial activity, cell growth, production of reactive oxygen species (ROS) and superoxide anions (O2(-)), catalase and superoxide dismutase activities were determined. The ability of the oxysterols to induce increases in Ca(2+) was measured after 10min and 24h of treatment using fura-2 videomicroscopy and Von Kossa staining, respectively. Cholesterol, 7-ketocholesterol, 7β-hydroxycholesterol, and 24(S)-hydroxycholesterol (100μM) induced mitochondrial dysfunction, cell growth inhibition, ROS overproduction and cell death. A slight increase in the percentage of cells with condensed and/or fragmented nuclei, characteristic of apoptotic cells, was detected. With 27-hydroxycholesterol, a marked increase of O2(-) was observed. Increases in intracellular Ca(2+) were only found with 7-ketocholesterol, 7β-hydroxycholesterol, 24(S)-hydroxycholesterol and 27-hydroxycholesterol. Pre-treatment with docosahexaenoic acid showed some protective effects depending on the oxysterol considered. According to the present data, 7-ketocholesterol, 7β-hydroxycholesterol, 24(S)-hydroxycholesterol and 27-hydroxycholesterol could favor neurodegeneration by their abilities to induce mitochondrial dysfunctions, oxidative stress and/or cell death associated or not with increases in cytosolic calcium levels. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Cord blood insulinoma-associated protein 2 autoantibodies are associated with increased risk of type 1 diabetes in the population-based Diabetes Prediction in Skåne study.

    Science.gov (United States)

    Lundgren, Markus; Lynch, Kristian; Larsson, Christer; Elding Larsson, Helena

    2015-01-01

    The aim of this study was to examine the effect of cord blood autoantibodies on the risk for type 1 diabetes in children followed prospectively from birth. The Diabetes Prediction in Skåne (DiPiS) study consists of 35,853 children from the general population born during 2000-2004. Samples were collected at birth and analysed for HLA genotypes and autoantibodies to glutamate decarboxylase 65 (GAD65), insulin and insulinoma-associated protein 2 (IA-2). After adjusting for HLA, sex, maternal age and parental type 1 diabetes, independent associations with risk of diabetes were assessed using multivariate Cox proportional hazards models. In total, 151 children (0.4%) had developed type 1 diabetes by the end of 2013 at a median age of 5.8 years (0.8-12.2 years). In the multivariate analysis, the presence of IA-2 autoantibodies (IA-2A) in cord blood (HR 6.88, 95% CI 1.46,32.4; p = 0.003), but not maternal diabetes (HR 1.38, 95% CI 0.24,7.84; p = 0.71), was associated with risk of developing type 1 diabetes. No increased risk could be seen for the presence of autoantibodies to GAD65 or insulin. Our study indicates that the presence of cord blood IA-2A superimposes maternal diabetes and other cord blood islet autoantibodies as a predictor of type 1 diabetes development in the child. These findings may be of significance for future screening and study protocols on type 1 diabetes prediction.

  19. Differential involvement of dopamine D-1 and D-2 receptors in the circling behaviour induced by apomorphine, SK & F 38393, pergolide and LY 171555 in 6-hydroxydopamine-lesioned rats.

    Science.gov (United States)

    Arnt, J; Hyttel, J

    1985-01-01

    The antagonistic effect of dopamine (DA) D-1 and D-2 antagonists against circling behaviour induced by various DA agonists in 6-OHDA-lesioned rats has been investigated. DA D-1/D-2 selectivity of agonists in vitro was measured by the stimulatory effect on DA-sensitive adenylate cyclase in rat striatal homogenates (D-1), the inhibitory effect on electrically-induced release of 3H-DA in rabbit striatal slices (D-2) and the affinity to 3H-piflutixol (D-1) and 3H-spiroperidol (D-2) binding sites in rat striatal membranes. The contralateral circling behaviour induced by the DA D-1 agonist SK & F 38393 was blocked by the DA D-1 antagonist, SCH 23390, and by the mixed DA D-1/D-2 antagonist cis(Z)-flupentixol, but was not influenced by the DA D-2 antagonists spiroperidol and clebopride. In contrast, circling behaviour induced by the preferential DA D-2 agonists pergolide and LY 171555 was blocked by clebopride, spiroperidol, and cis(Z)-flupentixol, but weakly or not influenced by SCH 23390. Apomorphine-induced circling behaviour was blocked by cis(Z)-flupentixol, partially antagonized by SCH 23390 and clebopride but not inhibited by spiroperidol, although the time-course of circling was changed. Combinations of SCH 23390 with spiroperidol or clebopride in low doses completely blocked the effect of apomorphine. These results indicate that DA D-1 and D-2 receptors mediate circling behaviour through separate mechanisms which can be independently manipulated with respective agonists and antagonists.(ABSTRACT TRUNCATED AT 250 WORDS)

  20. Effect of normalization methods on the performance of supervised learning algorithms applied to HTSeq-FPKM-UQ data sets: 7SK RNA expression as a predictor of survival in patients with colon adenocarcinoma.

    Science.gov (United States)

    Shahriyari, Leili

    2017-11-03

    One of the main challenges in machine learning (ML) is choosing an appropriate normalization method. Here, we examine the effect of various normalization methods on analyzing FPKM upper quartile (FPKM-UQ) RNA sequencing data sets. We collect the HTSeq-FPKM-UQ files of patients with colon adenocarcinoma from TCGA-COAD project. We compare three most common normalization methods: scaling, standardizing using z-score and vector normalization by visualizing the normalized data set and evaluating the performance of 12 supervised learning algorithms on the normalized data set. Additionally, for each of these normalization methods, we use two different normalization strategies: normalizing samples (files) or normalizing features (genes). Regardless of normalization methods, a support vector machine (SVM) model with the radial basis function kernel had the maximum accuracy (78%) in predicting the vital status of the patients. However, the fitting time of SVM depended on the normalization methods, and it reached its minimum fitting time when files were normalized to the unit length. Furthermore, among all 12 learning algorithms and 6 different normalization techniques, the Bernoulli naive Bayes model after standardizing files had the best performance in terms of maximizing the accuracy as well as minimizing the fitting time. We also investigated the effect of dimensionality reduction methods on the performance of the supervised ML algorithms. Reducing the dimension of the data set did not increase the maximum accuracy of 78%. However, it leaded to discovery of the 7SK RNA gene expression as a predictor of survival in patients with colon adenocarcinoma with accuracy of 78%. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com

  1. Hypothyroidism After Head-and-Neck Radiotherapy in Children and Adolescents: Preliminary Results of the 'Registry for the Evaluation of Side Effects After Radiotherapy in Childhood and Adolescence' (RiSK)

    Energy Technology Data Exchange (ETDEWEB)

    Boelling, Tobias, E-mail: Tobias.Boelling@uni-muenster.de [Department of Radiotherapy, University Hospital of Muenster, Muenster (Germany); Department of Radiotherapy, Paracelsus Clinic Osnabrueck, Osnabrueck (Germany); Geisenheiser, Alina [Department of Radiotherapy, University Hospital of Muenster, Muenster (Germany); Pape, Hildegard [Department of Radiotherapy, University Hospital of Duesseldorf, Duesseldorf (Germany); Martini, Carmen [Department of Radiotherapy, University Hospital of Freiburg, Freiburg (Germany); Ruebe, Christian [Department of Radiotherapy, University Hospital of Homburg/Saar, Homburg/Saar (Germany); Timmermann, Beate [Center for Proton Radiation Therapy, Paul-Scherrer-Institute, Villigen (Switzerland); Fischedick, Karin [Department of Radiotherapy, University of Aachen, Aachen (Germany); Kortmann, Rolf-Dieter [Department of Radiotherapy, University of Leipzig, Leipzig (Germany); Gerss, Joachim; Koch, Raphael [Department of Medical Informatics and Biomathematics, University of Muenster, Muenster (Germany); Center for Clinical Trials, University Hospital of Muenster, Muenster (Germany); Willich, Normann [Department of Radiotherapy, University Hospital of Muenster, Muenster (Germany)

    2011-12-01

    Purpose: The 'Registry for the Evaluation of Side Effects After Radiotherapy in Childhood and Adolescence' (RiSK) has been established to prospectively characterize dose-volume effects of radiation in terms of side effects. The aim of this analysis was to characterize the function of the thyroid gland after radiotherapy to the head-and-neck region in children and adolescents. Methods and Materials: Detailed information regarding radiation doses to at-risk organs has been collected across Germany since 2001. Thyroid function was evaluated by blood value examinations of thyroid-stimulating hormone, triiodothyronine, and thyroxine. Information regarding thyroid hormone substitution was requested from the treating physicians. Results: Until May 2009, 1,086 patients from 62 centers were recruited, including 404 patients (median age, 10.9 years) who had received radiotherapy to the thyroid gland and/or hypophysis. Follow-up information was available for 264 patients (60.9%; median follow-up, 40 months), with 60 patients (22.7%) showing pathologic values. In comparison to patients treated with prophylactic cranial irradiation (median dose, 12 Gy), patients with radiation doses of 15 to 25 Gy to the thyroid gland had a hazard ratio of 3.072 (p = 0.002) for the development of pathologic thyroid blood values. Patients with greater than 25 Gy to the thyroid gland and patients who underwent craniospinal irradiation had hazard ratios of 3.768 (p = 0.009) and 5.674 (p < 0.001), respectively. The cumulative incidence of thyroid hormone substitution therapy did not differ between defined subgroups. Conclusions: Radiation-induced thyroid function impairment, including damage to the thyroid gland and/or hypophysis, can frequently be observed after radiotherapy in children. A structured follow-up examination is advised.

  2. Skønlitteraturen som opposition

    DEFF Research Database (Denmark)

    Larsen, Pelle Oliver

    2011-01-01

    Troels Troels-Lund er kendt som foregangsmanden i den første kulturhistoriske bølge i Danmark fra omkring 1880. Hans form for kulturhistorie vandt imidlertid aldrig for alvor faghistorikernes anerkendelse, og Troels-Lund blev udgrænset. Denne artikel går tilbage til 1870’erne og indeholder en ana...

  3. Maskinell skörd av jordgubbar

    OpenAIRE

    Olander, Sven

    1985-01-01

    Mechanical harvesting of strawberries has been at the experimental stage since the early l960's, but has not attained any commercial success until recently. One of the main problems has been that the strawberries ripen successively during a couple of weeks, and that most harvesters are based on the once-over principle. This results in a decrease in yield at the same time as a relatively large quantity of green strawberries is harvested. It has frequently been considered profitable to hand-pic...

  4. Humboldt SK pilot biodigester receives funding

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2005-09-30

    The Canada-Saskatchewan Western Economic Partnership Agreement (WEPA) will provide funding for Canada's first pilot-scale biodigester to be built in Humboldt, Saskatchewan. The $208,138 pilot facility will use household garbage as well as agricultural waste such as manure and livestock operations, abattoirs and food processing to create heat or power and fertilizer. Support for this bio-energy facility, which could also reduce greenhouse gases, will come from the Prairie Agricultural Machinery Institute which has received $186,138 from Canada and Saskatchewan in the form of cost-shared federal-provincial funding. The pilot plant will test different combinations of waste material feedstocks and the characteristics of the resulting gas end products. The pilot facility will also provide design information for full-scale biodigester manufacturers in Canada. It is expected that 25 full-scale biodigesters will be constructed in Canada in the near future.

  5. Blýská se…

    Czech Academy of Sciences Publication Activity Database

    Kolmašová, Ivana

    2015-01-01

    Roč. 60, č. 2 (2015), s. 123-132 ISSN 0032-2423 Institutional support: RVO:68378289 Keywords : lightning discharges * thundercloud * transient luminous events Subject RIV: DG - Athmosphere Sciences, Meteorology http://www.dml.cz/handle/10338.dmlcz/144406

  6. Skærpet bevidsthed om supervision

    DEFF Research Database (Denmark)

    Pedersen, Inge Nygaard

    2002-01-01

    This article presents a historical survey of the initiatives which have taken place in european music therapy towards developing a deeper consciousness about supervision. Supervision as a disciplin in music therapy training, as a maintenance of music therapy profession and as a postgraduate...... training for examined music therapists. Definitions are presented and methods developed by working groups in european music therapy supervision are presented....

  7. Rideudstyr og skøjter

    DEFF Research Database (Denmark)

    Roesdahl, Else; Pedersen, Anne

    2014-01-01

    Finds from Aggersborg: snaffle-bit, buckles, strap-slides, strap-mounts, iron loop, strap-pendants, bell, enamelled roundel, spurs, animal-head - fragment of a spur?, calkin, ice-skates......Finds from Aggersborg: snaffle-bit, buckles, strap-slides, strap-mounts, iron loop, strap-pendants, bell, enamelled roundel, spurs, animal-head - fragment of a spur?, calkin, ice-skates...

  8. Fra PC til Skærm

    DEFF Research Database (Denmark)

    Ebbesen, Toke Riis

    2015-01-01

    Denne analyse afdækker iMac’ens designkulturelle betydning ved først at eksponere den eksisterende stereotypiske kulturelle forståelse af computer-typer. I lyset heraf vises det, hvordan Apple konsekvent har arbejdet med at fremstille iMac’en er som en helt ny computertype, først på den oprindeli...

  9. Fra SK 4b til MH 17

    DEFF Research Database (Denmark)

    Stræde, Therkel

    2015-01-01

    Om den nazistiske besættelsespolitik i Sydøstukraine og Sonderkommando 4b's udryddelse af jøder på den egn, hvor Malaysian Airlines MH 17 styrtede ned den 17. juli 2014......Om den nazistiske besættelsespolitik i Sydøstukraine og Sonderkommando 4b's udryddelse af jøder på den egn, hvor Malaysian Airlines MH 17 styrtede ned den 17. juli 2014...

  10. SK policies the view of nuclear municipalities

    International Nuclear Information System (INIS)

    D'abadal, M.V.

    2002-01-01

    The nuclear policies of the most important countries in Western Europe, already influenced by the evolution of the public opinion, have experimented strong changes together with political evolution. The future of NNP in some important countries is doubtful, as for the case of Sweden, Germany, Spain, Holland, Belgium, The large democratic discussions that affect permanently our society, as well as the new society of information that is being implanted, have turned the decision making processes into what is referred to as public participation and transparency, especially when these affect the environment or the immediate future of the citizens. The installation policies of nuclear plants are very similar in all the countries. Most of them are located in low density population areas, with low activity rate, high rate of elder people. These territories have many water resources, low communication infrastructure level, etc. So the typographic aspects of the European municipalities are alike (in eastern countries as well). Nuclear energy whose existence is sometimes called in question by press media, citizens and inhabitants, needs full agreement within the territory in order to work at its best. Moreover, the territory on which the plant is installed must have the necessary means of infrastructure ( development from a social and economic point of view) as well as the dues for its future and for a new positive reality as far as the citizens are concerned in order to face the corresponding challenges. Having got to this point, a territorial debate should be focused on the balance between the state's general interest and the local one as normalised operation of nuclear facilities is and will be possible only in a context of mutual respect. The new European political map and the last governmental decisions in energetic strategies grant more value to the opinion of the local authorities on the territories affected by these facilities. So, in order to express their opinion, these authorities should gather within a European forum where the communication would become a lot more fluent. With this goal the European Municipalities created the GMF, a group of municipalities which think that they have to take part in decision making process in order to defend any interest dealing with the people living in nuclear territories. The will of GMF is to work together in favour of the security and the quality of life, which means socio-economic progress for their citizens. The municipalities wish to stay away from the general and typical debate about yes or no to nuclear energy. (author)

  11. A risk prediction model for the assessment and triage of women with hypertensive disorders of pregnancy in low-resourced settings: the miniPIERS (Pre-eclampsia Integrated Estimate of RiSk multi-country prospective cohort study.

    Directory of Open Access Journals (Sweden)

    Beth A Payne

    2014-01-01

    Full Text Available Pre-eclampsia/eclampsia are leading causes of maternal mortality and morbidity, particularly in low- and middle- income countries (LMICs. We developed the miniPIERS risk prediction model to provide a simple, evidence-based tool to identify pregnant women in LMICs at increased risk of death or major hypertensive-related complications.From 1 July 2008 to 31 March 2012, in five LMICs, data were collected prospectively on 2,081 women with any hypertensive disorder of pregnancy admitted to a participating centre. Candidate predictors collected within 24 hours of admission were entered into a step-wise backward elimination logistic regression model to predict a composite adverse maternal outcome within 48 hours of admission. Model internal validation was accomplished by bootstrapping and external validation was completed using data from 1,300 women in the Pre-eclampsia Integrated Estimate of RiSk (fullPIERS dataset. Predictive performance was assessed for calibration, discrimination, and stratification capacity. The final miniPIERS model included: parity (nulliparous versus multiparous; gestational age on admission; headache/visual disturbances; chest pain/dyspnoea; vaginal bleeding with abdominal pain; systolic blood pressure; and dipstick proteinuria. The miniPIERS model was well-calibrated and had an area under the receiver operating characteristic curve (AUC ROC of 0.768 (95% CI 0.735-0.801 with an average optimism of 0.037. External validation AUC ROC was 0.713 (95% CI 0.658-0.768. A predicted probability ≥25% to define a positive test classified women with 85.5% accuracy. Limitations of this study include the composite outcome and the broad inclusion criteria of any hypertensive disorder of pregnancy. This broad approach was used to optimize model generalizability.The miniPIERS model shows reasonable ability to identify women at increased risk of adverse maternal outcomes associated with the hypertensive disorders of pregnancy. It could be

  12. Stýrinet: Nýsköpun innan Stjórnarráðsins. Nýtt verklag í samstarfi milli stjórnsýslustiga

    Directory of Open Access Journals (Sweden)

    Héðinn Unnsteinsson

    2012-06-01

    Full Text Available Í þessari grein er vakin athygli á nýju samskiptaformi sem er að ryðja sér til rúms innan stjórnsýslunnar með tilkomu stefnumörkunarinnar Ísland 2020. Stýrinet (e. governance network er hugtak sem æ fleiri fræðimenn og embættismenn hafa skoðað síðastliðin tíu ár sem mögulegan valkost við stigveldisstjórnsýslu og útvistaða stjórnsýslu, sem varð vinsæl í lok tíunda áratugarins með tilkomu nýsköpunar í ríkisrekstri (New Public Management. Samstarf þvert á ráðuneyti eru ekki ný af nálinni en með stefnuskjalinu Ísland 2020 eru sett fram verkefni sem krefjast annars konar vinnubragða en tíðkast hafa áður innan stjórnsýslunnar. Ísland 2020 hefur ýtt bæði undir samhentari stjórnsýslu með auknu samstarfi en krefst þess jafnframt að starfshópar starfi í anda stýrineta svo að sem mestur árangur megi verða af starfi þeirra. Í greininni er stýrinetskerfi, sem sett var upp innan Stjórnarráðsins og landshlutasamtaka í tengslum við sóknaráætlanir landshluta, skoðað. Tilraun er gerð til að meta hvort stýrinet séu betur til þess fallin en annars konar samstarf til að skila markvissari árangri við lausn verkefna.

  13. Zasady prezentacji składników pozostałego wyniku całościowego – praktykasprawozdawcza spółek należących do WIG 30 oraz DAX w latach 2012–2013 

    Directory of Open Access Journals (Sweden)

    Jacek Gad

    2015-04-01

    Full Text Available Celem artykułu jest wskazanie wpływu nowelizacji MSR 1 z roku 2012 na prezentację składników pozostałego wyniku całościowego przez spółki należące do indeksów WIG 30 oraz DAX. Część teoretyczną artykułu opracowano z wykorzystaniem studiów literatury krajowej i zagranicznej. Część empiryczną opracowano na podstawie informacji prezentowanych w raportach rocznych spółek z warszawskiego indeksu WIG 30 oraz niemieckiego indeksu DAX. Na przestrzeni lat 2012–2013 zmieniła się forma prezentacji składników pozostałego wyniku całościowego. Dotyczy to zarówno spółek z indeksu WIG 30, jak i DAX. Zidentyfikowano cztery główne warianty prezentacji składników pozostałego wyniku całościowego. Zdaniem autora kolejne warianty charakteryzują się rosnącą przejrzystością informacyjną sprawozdania z pozostałego wyniku całościowego. Praktyka sprawozdawcza spółek z indeksu DAX wyprzedza praktykę sprawozdawczą spółek z indeksu WIG 30. Do analizy uzyskanych wyników wykorzystano następujące statystyki opisowe: średnią arytmetyczną, odchylenie standardowe, medianę, percentyle. W ramach badania wykorzystano także wskaźnik podobieństwa struktur.

  14. Brak związku polimorfizmu –590 C/T genu IL-4 oraz –1082 A/G genu IL-10 z rozwojem atopowego zapalenia skóry

    Directory of Open Access Journals (Sweden)

    Anna Sysa-Jędrzejowska

    2010-09-01

    Full Text Available Wprowadzenie: Atopowe zapalenie skóry (AZS jest przewlekłą,zapalną dermatozą, często współistniejącą z innymi chorobami atopowymi,takimi jak astma, alergiczny nieżyt nosa bądź spojówek. Wśródwielu czynników etiopatogenetycznych podłoże genetyczne, obejmującepolimorfizmy i mutacje w genach kodujących białka zaangażowanew prawidłową budowę bariery naskórkowej oraz odpowiedź immunologiczną,jest przedmiotem wielu prac badawczych. W patogenezieAZS szczególną rolę przypisuje się cytokinom Th2-zależnym, w tyminterleukinom 4 i 10 (IL-4 i IL-10. Cel pracy: Ocena polimorfizmów regionu promotorowego –590 C/Tgenu IL-4 oraz 1082 A/G genu IL-10 u pacjentów z AZS zamieszkującychteren województwa łódzkiego. Materiał i metodyka: Grupę badawczą stanowiło 163 chorych na AZS,u których rozpoznanie choroby ustalono na podstawie kryteriów Hanifinai Rajki, natomiast grupę kontrolną 204 zdrowych wolontariuszy.Nasilenie procesu chorobowego u wszystkich chorych oceniono przyużyciu skali Rajki i Langelanda. Analizę wariantów polimorficznychw regionie promotorowym genów IL-4 i IL-10 przeprowadzono metodąRFLP-PCR z wykorzystaniem enzymów restrykcyjnych do wykrywaniazmian w sekwencji DNA. Wyniki: Nie wykazano istotnych statystycznie różnic w rozkładziegenotypów polimorfizmów –590 C/T dla IL-4 oraz –1082 A/G genuIL-10 między grupą pacjentów a grupą kontrolną (p > 0,05 dla wszystkichporównań. W badanych polimorfizmach genów kodujących IL-4i IL-10 rozkład genotypów nie różnił się statystycznie istotnie w grupiepacjentów mających łagodny przebieg AZS od chorych z AZS o umiarkowanymi ciężkim przebiegu. Nie stwierdzono (p > 0,05 równieżzwiązku między badanymi polimorfizmami genów IL-4 i IL-10 a wczesnymzachorowaniem na astmę. Wnioski: Brak pozytywnej korelacji między obecnością polimorfizmówgenów IL-4 oraz IL-10 a rozwojem AZS w badanej grupie chorychmoże świadczyć o odmienno

  15. Korelace mezi délkou lyžařské tratě a rychlostí vrcholových slalomářů Correlation between the length of the ski track and the velocity of top slalom skiers

    Directory of Open Access Journals (Sweden)

    Blaž Lešnik

    2007-02-01

    Full Text Available Teoreticky je nejlepší co nejkratší lyžařská dráha (lyžařská trať. V praxi je to však těžko proveditelné a kromě toho je to velmi riskantní. Může se proto stát pouze součástí taktiky těch nejlepších soutěžících. Pomocí kinematické analýzy byly stanoveny rozdíly mezi volbou lyžařské dráhy u jednotlivých soutěžících, a to na základě vzorku vrcholových soutěžících ve světovém poháru v Kranjske Gore. Snažili jsme se zjistit, zda soutěžící dosahovali na takové lyžařské dráze vyšší rychlosti, tedy zda dosahovali rychlejšího pohybu mezi slalomovými brankami a tudíž v konečném důsledku i lep ších časových výsledků. V první části našeho výzkumu bylo prokázáno, že žádný ze soutěžících neměl v prvních dvou po sobě následujících obratech extrémně krátkou lyžařskou dráhu. Bylo rovněž zjištěno, že soutěžící s nejkratšími lyžařskými drahami v prvním obratu měli zpravidla nejdelší lyžařské dráhy v obratu druhém a naopak. Na základě těchto zjištění bylo možno tvrdit, že za současných podmínek je mimořádně obtížné, a snad dokonce nemožné, lyžovat tak, aby lyžařský oblouk byl v několika po sobě následujících obratech velmi krátký. Druhá část našeho výzkumu se zaměřila na zjišťování korelace mezi trajektorií lyží a rychlostí lyžování. Výpočty korelací prokázaly statisticky významnou korelaci mezi průměrnou rychlostí lyžování a průměrnou vzdáleností délky lyžařské dráhy od osy y v celém měřeném úseku. Bylo zjištěno, že měřený prostor překonávali soutěžící s kratší, tedy přímější, lyžařskou dráhou rychleji. Theoretically, the shortest possible line of skiing (ski track is the best one. But in practice it is very difficult to carry this out and besides it is very risky. It can therefore be only a part of the tactics of the best competitors. With

  16. Skład kwasów tłuszczowych i twardość czekolad gorzkich o różnej zawartości miazgi kakaowej = Fatty acid composition and hardness of dark chocolates with different content of cacao mass

    Directory of Open Access Journals (Sweden)

    Renata Taradejna

    2015-09-01

    Wydział Nauki o Żywności, Uniwersytet Warmińsko-Mazurski w Olsztynie     Uniwersytet Warmińsko-Mazurski w Olsztynie Katedra Przetwórstwa i Chemii Surowców Roślinnych Pl. Cieszyński 1, 10-726 Olsztyn e-mail: beata.wronowska@uwm.edu.pl     Abstrakt W pracy analizowano zróżnicowanie dostępnych na rynku czekolad gorzkich pod względem składu kwasów tłuszczowych oraz twardości. Materiałem badawczym było 14 czekolad gorzkich pochodzących od różnych firm o zawartości miazgi kakaowej od 45% do 90%. Skład kwasów tłuszczowych oznaczono chromatograficznie, a twardość zmierzono w teście łamania za pomocą uniwersalnej maszyny testującej. Wykazano, że 6 czekolad zawierało w swoim składzie poniżej 65% miazgi kakaowej, nie spełniając wymagań określonych w polskiej normie dla czekolady gorzkiej. Pomimo tego, prawie wszystkie czekolady miały skład kwasów tłuszczowych typowy dla tłuszczu kakaowego; udziały kwasów oleinowego, stearynowego i palmitynowego wynosiły odpowiednio: 31,2-33,1%, 34,1-36,8% i 25,5-29,0%. Tylko w przypadku jednej czekolady stwierdzono wyższy udział kwasu palmitynowego (33,8%, natomiast niższy kwasów oleinowego i stearynowego (odpowiednio 29,4% i 32,9%. Badane czekolady gorzkie cechowały się zróżnicowaną twardością, na co wskazywały wartości siły kształtujące się w szerokim zakresie (24,2-85,6 N. Nie zaobserwowano zależności pomiędzy składem kwasów tłuszczowych a wartością siły potrzebnej do złamania kostki czekolady.   Słowa kluczowe: czekolada gorzka, miazga kakaowa, kwasy tłuszczowe, twardość.       Abstract The study analysed the diversity of commercially available dark chocolate in terms of fatty acid composition and hardness. The research material was 14 bitter chocolate from different companies about the content of cocoa mass from 45% to 90%. The fatty acid composition was determined by chromatography, and the hardness measured by the breaking test with the universal

  17. Trudności terapeutyczne u pacjenta z ciężką postacią młodzieńczego zapalenia skórno-mięśniowego powikłanego uogólnioną wapnicą

    Directory of Open Access Journals (Sweden)

    Joanna Szymańska-Kałuża

    2011-08-01

    Full Text Available Młodzieńcze zapalenie skórno-mięśniowe (MZSM to najczęstszaidiopatyczna miopatia zapalna wieku dziecięcego. Do charakterystycznychobjawów tej choroby należą: osłabienie mięśni, głównieobręczy barkowej lub biodrowej, oraz zmiany skórne w postaciobrzęku twarzy, heliotropowego zabarwienia powiek, zmian plamisto-rumieniowych na twarzy i klatce piersiowej. W badaniachlaboratoryjnych typowe dla MZSM są podwyższone wskaźnikiostrej fazy, wysoka aktywność enzymów mięśniowych (aminotransferaz,kinazy kreatynowej oraz dehydrogenazy mleczanowej,a także czasami obecność przeciwciał anty-Jo1. Badanie elektromiograficznewykazuje cechy pierwotnego uszkodzenia mięśni,a w obrazie histopatologicznym wycinka skórno-mięśniowegoodnotowuje się nacieki zapalne. U dzieci często obserwowane sątakże ogniska zwapnień w tkankach miękkich, prowadzące doatrofii mięśni i przykurczów stawowych.Leczeniem I rzutu u pacjentów z MZSM są glikokortykosteroidy,w przypadku nieskuteczności tej terapii stosuje się leki immunosupresyjnelub leki biologiczne, w tym immunoglobuliny.W pracy przedstawiono przypadek pacjenta z ciężkim przebiegiemMZSM powikłanego wapnicą, z uwzględnieniem objawów klinicznychi możliwości leczenia. Omówiono również trudności w doborzenajskuteczniejszej farmakoterapii dla tego pacjenta. U chorego– obecnie 14-letniego chłopca – występowały uogólnione zmianyskórne oraz bardzo gwałtownie narastające liczne złogi soli wapniaw skórze, tkance podskórnej, powięziach i mięśniach, które pojawiły się po 3 latach od wystąpienia pierwszych objawów chorobowych(ryc. 1–8. U chłopca z powodu wybitnie ciężkiego przebieguchoroby prowadzono agresywną konwencjonalną terapię(glikokortykosteroidy, metotreksat, cyklosporyna, pamidronian,która nie przynosiła zadowalających rezultatów i poprawy stanuklinicznego. Na podstawie doniesień z piśmiennictwa światowegopodjęto próbę leczenia

  18. Icelandic organizational culture; Clear strategic direction and intent but lack of coordination and integration Íslensk vinnustaðamenning. Skýr og markviss stefna en skortur á samhæfingu og samþættingu

    Directory of Open Access Journals (Sweden)

    Gylfi Dalmann Aðalsteinsson

    2010-12-01

    beint að megineinkennum vinnustaðamenningar íslenskra skipulagsheilda. Stuðst er við aðferð Denison til að varpa ljósi á íslenska vinnustaðamenningu. Ýmislegt bendir til að íslenskar skipulagsheildir eigi margt sameiginlegt þegar kemur að vinnustaðamenningu, að hægt sé að benda á sameiginleg megineinkenni í vinnustaðamenningu hérlendis. Helstu niðurstöður eru þær að skipulagsheildir sem skoðaðar voru hafa skýra og markvissa stefnu en ferlar virðast ekki vera vel skilgreindir, illa gengur að útfæra eða inn leiða stefnuna og ólíkum einingum innan skipulagsheildarinnar gengur illa að vinna saman. Í niðurstöðum rannsóknarnefndar Alþingis kemur fram að vinnustaðamenning og viðskiptasiðferði íslensku bankanna hafi orðið til þess að starfsemi þeirra fór að mörgu leyti á skjön við lög og reglur og í bága við eðlilega og heilbrigða viðskiptahætti. Því er áhugavert að skoða mælingar fyrir og eftir efnahagshrun. Þessar mælingar sýna ekki mikinn mun. Sá þáttur sem lendir á styrkleikabili á báðum tímabilunum er undirvíddin skýr og markviss stefna, þ.e. allir meðlimir skipulagsheildarinnar hafa skýra framtí��arsýn og þeir eru reiðubúnir að fara eftir stefnunni. Stefnan sé líkleg til að skapa samkeppnisyfirburði og hún virðist vera skýr í hugum starfsmanna. Á hinn bóginn er afar athyglisvert hversu lágt skor er í undirvíddinni samhæfing og samþætting. Það bendir til þess að þrátt fyrir skýra og markvissa stefnu séu ferlar ekki vel skilgreindir, illa gangi að útfæra eða innleiða stefnuna og ólíkum einingum innan skipulagsheildarinnar virðist ganga illa að vinna saman. Enn fremur er lágt skor í undirvíddunum gildi og breytingar sem getur verið vísbending um að sameiginleg gildi skorti innan skipulagsheilda og grunngildum sé ekki fylgt sem og til tregðu til að fara út í breytingar og taka áhættu.

  19. Ocena częstości występowania składowych zespołu metabolicznego w grupie pacjentów ze stabilną dusznicą bolesną kwalifikowanych do planowej koronarografii = Assessment of frequency of components of metabolic syndrome in patients with stable angina qualified for planning angiography

    Directory of Open Access Journals (Sweden)

    Katarzyna Sawicka

    2016-09-01

    Wstęp. Ze stabilną dusznicą bolesną wiąże się występowanie zespołu metabolicznego. Zespół metaboliczny to zbiór wzajemnie powiązanych czynników metabolicznych takich jak: upośledzona tolerancja glukozy, insulinooporność, hiperinsulinemia, otyłość, zaburzenia lipidowe, nadciśnienie tętnicze. Zespół ten stanowi główną przyczynę rozwoju chorób sercowo-naczyniowych o podłożu miażdżycowym i cukrzycy typu 2. Za rozwój zespołu metabolicznego odpowiedzialne są predyspozycje genetyczne oraz współdziałające czynniki środowiskowe, w tym brak aktywności fizycznej oraz wysokokaloryczna dieta [1]. Materiał i metoda badań. W niniejszej pracy posłużono się metodą sondażu diagnostycznego. Do gromadzenia informacji wykorzystano autorski kwestionariusz ankiety. Ankieta obejmowała 37 pytań, w tym 33 pytania zamknięte i 4 pytania półotwarte, w których istniała możliwość wpisania własnej odpowiedzi przez osoby ankietowane. Pytania pozwoliły na zebranie danych dotyczących cech demograficznych badanej populacji oraz ocenę zasobu wiedzy respondentów na temat poszczególnych składowych zespołu metabolicznego. Wyniki badań.  1. Uzyskane wyniki badań wskazują, że u 70% pacjentów ze stabilną dusznicą bolesną kwalifikowanych do planowej koronarografii występują składowe zespołu metabolicznego. 2. Istnieje zależność pomiędzy miejscem zamieszkania pacjenta, a występowaniem składowych zespołu metabolicznego: u osób mieszkających w mieście częściej występuje zespół metaboliczny. 3. Istnieje zależność pomiędzy stylem życia, a występowaniem składowych zespołu metabolicznego (u 54,5% ankietowanych, którzy prowadzą niezdrowy styl życia, istnieje problem występowania zespołu metabolicznego. 4. Nie istnieje zależność pomiędzy podejmowaniem aktywności fizycznej, a występowaniem składowych zespołu metabolicznego. 5. Nadciśnienie tętnicze jest najczęściej występującą składową zespo

  20. An analysis of Finnish skiing school students' academic education and athletic success [Analýza akademického vzdělání a sportovních úspěchů studentů finských lyžařských škol

    Directory of Open Access Journals (Sweden)

    Jan-Erik Romar

    2012-03-01

    Full Text Available BACKGROUND: Skiing boarding schools provide an opportunity to combine academic education and an athletic career. Many athletes select to go to a boarding school at an age of 16 because they have poor local training possibilities; however, this physical move away from home providesmany challenges. OBJECTIVE: The purpose of this study was to analyze academic education and athletic success of skiing school students in Finland. METHODS: The participants were 49 students (15 girls and 34 boys with an average age of 17 years. They came from three skiing boarding schools, two cross-country and one alpine school. Sixty percent of the alpine skiers were members of the junior national team while only six percent of the cross-country skiers. All participants completed a survey about study success and athletic performance. RESULTS: The results showed that 80% of the students extended their high school studies from three to four years. About 50% of all students were satisfied with their academic success. Fifty-four percent of alpine skiers and 15% of cross-country skiers indicated that their best athletic success were in international competitions. Almost all students perceived that skiing school helped by combining sport and school. However, only 40% of the alpine skiers and 62% of the cross-country skiers were satisfied with their present athletic success. Seventy-three percent of the alpine skiers felt that sport participation affected negatively their success in school. Success in sport, good training possibilities, skilled coaches and caring friends were reason for enjoying life in skiing boarding schools. CONCLUSIONS: These findings support the concept of skiing schools and suggest that there are many things to consider when combining education and an athletic career.[VÝCHODISKA: Lyžařské internátní školy nabízejí možnost spojit akademické vzdělání a sportovní kariéru. Mnozí sportovci odcházejí ve věku 16 let na internátní

  1. El silenciamiento de la proteína priónica celular (PrPC mediante RNA de interferencia (siRNA reduce la infección por HSV-1 y HSV-2 en células SK-SY5Y

    Directory of Open Access Journals (Sweden)

    Elizabeth Ortega-Soto

    2012-01-01

    Full Text Available Las encefalopatías espongiformes transmisibles (EETs son enfermedades neurodegenerativas fatales que afectan a humanos y ciertas especies animales. La hipótesis más aceptada indica que el agente infeccioso, denotado como prion y compuesto principalmente por la Proteína Priónica Scrapie (PrPSc, corresponde a una conformación anormal de una proteína codificada por el huésped denominada Proteína Priónica Celular (PrPC, cuya función es aún desconocida; sin embargo, la expresión ubicua de PrPC así como su elevado grado de conservación entre especies, sugieren un papel importante para esta proteína. En este trabajo se detectó a la PrPC en diferentes tipos celulares incluyendo un cultivo primario de células de peces (Tilapia, Oreochromis spp.. Además, basándonos en la secuencia de la PrPC humana, se diseñó un RNA de interferencia (siRNA con el fin de silenciar el gen PRNP en células neuronales SK-SY5Y. El siRNA diseñado inhibió la expresión de PrPC a lo largo de las 96 h post-transfección y las células silenciadas fueron menos susceptibles a la infección por HSV-1 y HSV-2, en comparación con células no transfectadas con el siRNA.

  2. Alexopoulos, Theodoros Benford's Law in Astronomy 639 Ali, Sk ...

    Indian Academy of Sciences (India)

    Since January 2016, the Journal of Astrophysics and Astronomy has moved to Continuous Article Publishing (CAP) mode. This means that each accepted article is being published immediately online with DOI and article citation ID with starting page number 1. Articles are also visible in Web of Science immediately.

  3. Komunikace programů vinařské turistiky

    OpenAIRE

    Krišpínová, Denisa

    2015-01-01

    The bachelor thesis focuses on marketing communication of wine tourism. The aim of this bachelor thesis is to analyse a communication mix of operators providing institutional program of wine tourism in South Moravian region and propose a unified communication strategy for Czech Republic. This thesis is divided into a theoretical and a practical part. The first part deals with explanation of main terms related to a marketing communication, which are applied in the second part. In the conclusio...

  4. Alexopoulos, Theodoros Benford's Law in Astronomy 639 Ali, Sk ...

    Indian Academy of Sciences (India)

    BetiCiciJoan

    Important Property of GRB Pulse: Power-Law Indices of Time Proper- ties on Energy. 535. Pi, F. P.. Astronomy Education Project for. Guangdong High Schools ... stricted Three-Body Problem with. Oblateness and Potential from a Belt. 107. On the Stability of L4,5 in the Relativis- tic R3BP with Radiating Secondary. 685. 752.

  5. Fronden betragtet i Trykkefrihedens skær

    DEFF Research Database (Denmark)

    Toftgaard, Anders

    2016-01-01

    Cardinal Mazarin depuis le 6 janvier jusqu’à la déclaration du 1er avril 1649, known as the Mascurat, the first edition of which was printed in 1649 and followed by a second, much enlarged edition in 1650. In this book, Naudé discusses the Mazarinades, political pamphlets attacking Cardinal Mazarin...... to the universal interests underlying Luxdorph’s library. One striking note does however compare the Fronde with the “revolution” in Denmark: in his notes, Luxdorph compares a passage in Naudé’s text about common people appropriating the art of printing with his own experience of his servant, who came up...

  6. Kulinářský cestovní ruch

    OpenAIRE

    ROUSOVÁ, Dagmar

    2018-01-01

    According to the changes in demand of tourism participants, contemporary trends are being constantly developed. One of them is a culinary tourism gastrotourism. Among the basic characteristics of the trend development is also motivation to bring such experience to the regional level. A description of history, a foundation of similar projects, organisations, and various events explain the initial idea. I addition, a descriptive mapping shows the number of entrepreneurs providing this service i...

  7. Visual Fashion-Product Search at SK Planet

    OpenAIRE

    Kim, Taewan; Kim, Seyeong; Na, Sangil; Kim, Hayoon; Kim, Moonki; Jeon, Byoung-Ki

    2016-01-01

    We build a large-scale visual search system which finds similar product images given a fashion item. Defining similarity among arbitrary fashion-products is still remains a challenging problem, even there is no exact ground-truth. To resolve this problem, we define more than 90 fashion-related attributes, and combination of these attributes can represent thousands of unique fashion-styles. The fashion-attributes are one of the ingredients to define semantic similarity among fashion-product im...

  8. Samfundsmæssige skævheder i klassen

    DEFF Research Database (Denmark)

    Brinkkjær, Ulf

    2014-01-01

    Sociale forskelle og dermed forskelle i både børns og læreres baggrundsmiljøer betyder noget for skolens distribution af viden og dermed for, hvad som læres af hvem og på hvilke måder. Denne indirekte form for ledelse i klasserummet kanaliserer skolesucces for det bedre stillede mellemlag. Kapitl...

  9. En skåltale for det tyske demokrati?

    DEFF Research Database (Denmark)

    Peters, Rikke

    2009-01-01

      The essay introduces and discusses Kurt Sontheimer's article: "The Continuity in German antidemocratic Thought. From the Weimar Republic to the Federal Republic" published in the anthology: Gessenharter & Pfeiffer (eds): Die Neue Rechte - eine Gefahr für die Demokratie?, Wiesbaden 2004. Kurt...... Sontheimer is one of the leading experts in modern political thought and has in several books examined the various nationalist and antidemocratic movements in 20th century German history. Today national conservative and elitist ideas can be found in a number of publications, and a growing number...... of intellectuals are attempting to make nationalism acceptable to the political centre. Sontheimer's warning against the revival of a new nationalism may therefore very well be appreciated when celebrating the 60s anniversary of The Federal Republic of Germany in 2009....

  10. Marketing mateřské školy

    OpenAIRE

    Vondráková, Ivana

    2008-01-01

    This bachelor's paper is to be aimed at marketing mix usage and it is application in managing position of the principal of Kindergarten. It is theoretical part explains the main terms used in connection to marketing and marketing mix. Practical part of this paper is to describe strong and weak point of marketing mix and sufficient process to effective use of these skills. The ending summarizes all the above mentioned chapters.

  11. Hatten af for Cohens skønheder og skyggesider

    DEFF Research Database (Denmark)

    Mortensen, Lise Majgaard

    2011-01-01

    Der er højt til loftet i Aarhus Teaters nye teaterkoncert, som behandler selveste Leonard Cohens lyriske univers. Det er svært at lade være med at sammenligne forestillingen her med Nick Cave Teaterkoncerten, der gik sin sejrsgang i 2007 og 2008. Men det skal man ikke. Instruktøren er den samme....... Men hvor ’Nick Cave’ var rå, grim, provokerende og uanstændig, er ’Leonard Cohen’ en skrøbelig gentleman med en skyggeside....

  12. Highlights of SK Mitra's Life-long Achievements

    Indian Academy of Sciences (India)

    Introduction of 'wireless' as an elective subjectforthe M.Sc. course in physics (this marked the beginning of radio science teaching in India), development of the ... Construction and installation of a radio transmitter with amateur's call sign 2CZ,.

  13. Investigations of an intense aerosol loading during 2007 cyclone SIDR - A study using satellite data and ground measurements over Indian region

    Digital Repository Service at National Institute of Oceanography (India)

    Badarinath, K.V.S.; Kharol, S.K.; Sharma, A.R.; Ramaswamy, V.; Kaskaoutis, D.G.; Kambezidis, H.D.

    upwind of BoB consists of cement factories, thermal power plants (coal- based), steel plants, etc (Satheesh, 2002). This general characteristic ofaerosolshoweverwasstronglymodifiedduringthepassageofthe cyclone SIDR and thus rendering its investigation a...

  14. Associateship | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Associate Profile. Period: 2001–2005. Satheesh, Dr S K . Date of birth: 1 May 1970. Specialization: Aerosols in Climate Address during Associateship: Centre for Atmospheric & Oceanic, Sciences, Indian Institute of Science, Bangalore 560 012

  15. Journal of Chemical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    , Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru N. Jayaraman, Indian Institute of Science, Bengaluru K C Kumara Swamy, University of Hyderabad, Hyderabad J N Moorthy, Indian Institute of Technology, Kanpur

  16. Badania epidemiologiczne jako podstawa informacyjna rozpoznania chorób alergicznych układu oddechowego i skóry u dzieci w wieku 6–7 i 13–14 lat w rejonie grodzieńskim

    Directory of Open Access Journals (Sweden)

    Andrei Shpakou

    2015-03-01

    Full Text Available Wstęp: Duże rozpowszechnienie, skutki medyczne i społeczne, wpływ na jakość życia oraz znaczący koszt chorób alergicznych uzasadniają liczne działania na rzecz ich profilaktyki. Nie ma wątpliwości, iż oficjalne statystyki mogą nie odpowiadać rzeczywistej częstotliwości występowania chorób alergicznych. Cel pracy: Celem pracy jest ocena częstotliwości występowania astmy i chorób alergicznych, głównych objawów alergicznych i oddechowych wśród miejskich i wiejskich dzieci w wieku 6–7 i 13–14 lat (w grupach wiekowych według badań ISAAC w rejonie Grodna. Materiał i metody: W badaniu kwestionariuszowym (na podstawie ankiety ISAAC wzięło udział 2187 rodziców dzieci w wieku 6–7 i 13–14 lat z Grodna i  jego okolic (1091 dzieci z miasta i 1096 ze wsi. Wśród nich 955 dzieci było w wieku 6–7 lat, a 1232 w wieku 13–14 lat. Wiedząc o tym, że astmę oskrzelową można podejrzewać u osób, które odczuwają duszności z towarzyszącymi świstami, uciskiem w klatce piersiowej lub kaszlem i chorobami alergicznymi (alergiczny nieżyt nosa i atopowe zapalenie skóry, przeanalizowano częstotliwość występowania tych objawów i chorób wśród dzieci. Wyniki: Wykazano niską częstotliwość występowania astmy wśród dzieci w  standaryzowanych grupach wiekowych. Rodzice 30 dzieci (1,37% badanych potwierdzają istnienie choroby u dzieci na podstawie jej zdiagnozowania przez lekarza. Wskaźnik występowania astmy wyniósł wśród dzieci miejskich 1,74% (19 osób, a wiejskich – 1,0% (11 dzieci. Po głębszej analizie odpowiedzi ustalono, że 60 dzieci (2,74% miało w ciągu ostatnich 12 miesięcy świsty w klatce piersiowej i napady kaszlu. Częstotliwość występowania alergicznego nieżytu nosa i atopowego zapalenia skóry wynosiła około 4% i 10%. Choroby te częściej spotykano u dzieci chorych na astmę. Wnioski: Ponieważ rozpoznawalność astmy u dzieci na

  17. Nasiona szałwii hiszpańskiej (Salvia Hispanica L. jako źródło składników wykazujących dobroczynny wpływ na ludzki organizm = Chia seeds (Salvia hispanica L. as a source of ingredients with beneficial influence on human organism

    Directory of Open Access Journals (Sweden)

    Grzegorz Dąbrowski

    2015-09-01

      Uniwersytet Warmińsko-Mazurski w Olsztynie Katedra Przetwórstwa i Chemii Surowców Roślinnych Pl. Cieszyński 1, 10-726 Olsztyn e-mail: grzegorz.dabrowski@uwm.edu.pl   Abstrakt Nasiona szałwii hiszpańskiej były jednym z najważniejszych źródeł pożywienia cywilizacji środkowoamerykańskich z czasów przedkolumbijskich. Z żywieniowego punktu widzenia bardzo interesujący jest ich skład chemiczny. Zawierają one duże ilości polienowych kwasów tłuszczowych. Najbardziej wartościowy jest kwas α-linolenowy, który ma między innymi zdolność zapobiegania chorobie wieńcowej i cukrzycy. Obecność kwasów fenolowych, tokoferoli i flawonoidów pomaga zapewnić stabilność oksydacyjną tłuszczu z nasion szałwii. Zawierają one duże ilości frakcji błonnika pokarmowego. Zawartość nierozpuszczalnej frakcji błonnika jest od 4 do 5 razy wyższa niż frakcji nierozpuszczalnej. Stosunek ten jest korzystny i może odgrywać rolę przy obniżaniu poziomu cholesterolu we krwi. Frakcja białkowa nasion szałwii zawiera duże ilości aminokwasów egzogennych i może być źródłem bioaktywnych peptydów. Słowa kluczowe: Salvia hispanica L., polienowe kwasy tłuszczowe, włókno pokarmowe, przeciwutleniacze, białka.   Abstract Chia (Salvia hispanica L. seeds were one of the most important sources of food for Central American civilizations in pre-Columbian times. The chemical composition of chia seeds is very interesting from nutritional point of view. It contains large amounts of polyunsaturated fatty acids. Most valuable is α-linoleic acid that can be helpful in between in coronary heart disease or diabetes prevention. The presence of phenolic acids, tocopherols and flavonoids helps to provide oxidative stability of oil from chia seeds. Chia seeds contain large amounts of dietary fiber. Content of insoluble fraction of dietary fiber is 4-5 times higher than soluble. It is good proportion and it can be helpful in lowering cholesterol content in blood

  18. Centile charts of motor development in girls aged between 7.5–19.5 from the Kujawsko-Pomorskie district Centilové stupnice motorického vývoje u dívek ve věku 7,5–19,5 roku z kujavsko-pomořského kraje

    Directory of Open Access Journals (Sweden)

    Marek Napierała

    2006-02-01

    Full Text Available The aim of the research was to present motor development with the help of centile charts, where the centile curves determine particular parts of the population: 3, 10, 25, 50, 75, 90 and 97 percentile. The research was conducted in the spring of 2001. A survey of 11 803 girls aged between 7.5 and 19.5 years old, from various types of rural and urban schools of the Kujawsko-Pomorskie district, was conducted. Centile charts make it possible to observe the process of the development of the examined characteristic, to answer the question as to whether an individual is at the level which is characteristic of a certain age, and, in case of aberrations, to come to a conclusion that the process of growth is disturbed. While determining the centile position one can become familiar with the level of development of an individual against calendar individuals of the same age. The value of C50 determines the calendar age of an individual. The results of the empiric research shows that the curve of the individual development of a child "wanders" within some channels, which is quite normal and does not mean a distortion of development. Cílem výzkumů bylo představit motorický vývoj pomocí centilových stupnic, kdy centilové křivky určují jisté části populace: 3, 10, 25, 50, 75, 90 a 97 percentil. Výzkumy byly prováděny na jaře roku 2001. Týkaly se 11 803 dívek ve věku mezi 7,5 a 19,5 lety z různých typů venkovských a městských škol kujavsko-pomořského kraje. Centilové stupnice umožňují sledovat proces vývoje zkoumaných charakteristik, zodpovědět na otázku, zda se jednotlivec nachází na úrovni charakteristické pro určitý věk a – v případě odchylek – dospět k závěru, že proces růstu je narušený. Při stanovování centilové pozice je možno poznat úroveň vývoje jednotlivce ve srovnání s jednotlivci téhož kalendářního věku. Kalendářní věk jednotlivce určuje hodnota C50. Výsledky empirick

  19. Čtenářský koutek a jeho role v rozvoji čtenářské pregramotnosti

    OpenAIRE

    Vohralíková, Zuzana

    2016-01-01

    This thesis is in its theoretical part focusing on reading literacy, reading pre-literacy, projects to promote literacy in the Czech Republic and reading corner in kindergarten.The research is based on qualitative research using a strategy of multiple case studies of purposely chosen kindergartens and methods of observation, interviews and content analysis.The aim of this research was to determine how and for what purpose the reading corner in kindergartens is used. The basic research questio...

  20. Skøjteskader. En undersøgelse of skademønstret i en dansk kommunal skøjtehal

    DEFF Research Database (Denmark)

    Johannsen, H G; Mikkelsen, J.B.; Fricke, A

    1997-01-01

    . About 25 per cent of the injuries were lesions the face or head. In seven cases the patient had been cut on the hand by the blade of another participant's skate, two patients had tendon lesions and in 16 cases the patient had sustained a fracture of the forearm or hand as the result of a fall. Many...

  1. Pražské Jezulátko v tiskařské produkci před rokem 1800

    Czech Academy of Sciences Publication Activity Database

    Baďurová, Anežka

    2012-01-01

    Roč. 57, 1/2 (2012), s. 53-74 ISSN 0036-5351 Institutional research plan: CEZ:AV0Z70830501 Keywords : Bohemica 1500-1800 * Jesus Baby of Prague * Discalced Carmelites * recatholisation Subject RIV: AB - History

  2. Lejringsbetingede skæve hoveder hos spædbørn kan forebygges

    DEFF Research Database (Denmark)

    Knudsen, Birgit; Christensen, Karen; Baagøe, Susanne

    2011-01-01

    The prevalence of positional plagiocephaly is increasing. A systematic database search (Cochrane, MEDLINE, Embase, Cinahl and PEDro), critical appraisal and systematic evaluation of the quality of 14 selected studies show that the following are certain risk factors for the development of position...... plagiocephaly: supine sleeping position, positional preference, one-sided handling of the child, male sex, delayed motor development, reduced neck mobility, too little "tummy time", firstborn, multiple births and premature children. Recommendations for prevention are provided....

  3. Zakladateľský plán japonskej reštaurácie

    OpenAIRE

    Koža, Michal

    2012-01-01

    The goal of this thesis is to create a business plan for a Japanese restaurant in the centre of Prague. Theoretical part of this thesis focuses the entrepreneurship activity itself and describes the structure of a business plan. The following practical part mainly analyses the macro and microenvironment of the restaurant, other competing restaurants, marketing strategy and further describes the restaurant. Considerable part of the thesis is dedicated to the analysis of current trends at the g...

  4. Zmiany skórne w przebiegu ostrej białaczki szpikowej – opis przypadku

    Directory of Open Access Journals (Sweden)

    Waldemar Placek

    2011-08-01

    Full Text Available Introduction. Acute myeloid leukaemia is a malignant neoplastic diseaseof white blood cells. About 80% of acute leukaemias in adult arediagnosed as myeloid leukaemias. The presence of transformed cellclones in bone marrow and blood, which originate from very earlystages of myelopoiesis, is characteristic. Beside systemic symptoms(anaemia, thrombocytopenia there are infiltrations in other organs,including skin. Skin lesions are most frequently non-specific (pruritus,skin spots, erythema multiforme, bullous pemphigoid but can be alsospecific (skin tumours. Cases of diffuse xanthomatoses and myeloidsarcoma have also been found in patients with acute myeloidleukaemia. Skin lesions are present more often in lymphocytic thanmyeloid leukaemias. They may precede the disease or its relapse, occurconcomitantly or after systemic symptoms, and sometimes may be ofprognostic value.Objective. To present the case of a patient in whom skin lesions werethe cause of detailed examinations, which revealed acute myeloidleukaemia.Case report. We present a case of a 61-year old patient with purplebrownnodular lesions 1-3 cm in diameter on the face, scalp skin, trunk,limbs and the oral cavity’s mucous membranes with coexistent intensepruritus and generalized lymphadenopathy. The first skin lesionsoccurred three months earlier. During hospitalization in the Departmentof Dermatology the renewal of blasts in the circulatory blood wasobserved and skin biopsy revealed a large amount of mononuclearcells. The patient was directed to the haematological ward, where thediagnosis of acute myeloid leukaemia was established.Conclusions. Our case indicates that the presence of skin lesions andthorough diagnostic procedures may contribute to the diagnosis ofmalignant neoplastic diseases of white blood cells.

  5. Kosmeceutyki w pielęgnacji skóry kobiet w okresie menopauzalnym

    Directory of Open Access Journals (Sweden)

    Dorota Wojnowska

    2011-08-01

    Full Text Available The term “cosmeceuticals” was introduced by dermatologist Albert Kligman more than 25 years ago todefine more active cosmetics with drug-like or therapeutical effects. Cosmeceuticals are dedicated to specificesthetic skin problems, for example wrinkles or hyperpigmentation. Mature skin showing signs of hormonalaging needs cosmetics with active ingredients which act protectively and improve appearance. Cosmeceuticalsare typically claimed to deeply moisturize and regenerate skin by beneficial ingredients: retinoids, hydroxy acids,antioxidants, depigmenting agents, botanical extracts, growth factors, fitoestrogens and peptides. The textdiscusses and focuses on the most important groups of active agents reversing skin aging symptoms.

  6. Astronomický ciferník pražského orloje

    Czech Academy of Sciences Publication Activity Database

    Křížek, Filip; Křížek, Michal

    2011-01-01

    Roč. 86, č. 1 (2011), s. 1-6 ISSN 0035-9343 Institutional research plan: CEZ:AV0Z10190503; CEZ:AV0Z10480505 Keywords : great circle * Ptolemy theorem * stereographic projection Subject RIV: BA - General Mathematics

  7. Skønhed og rædsel i den nye Pharma Science Building

    DEFF Research Database (Denmark)

    Reeh, Henrik

    2016-01-01

    Reportage fra et fælles besøg i den nyligt indviede PharmaScience Building, Københavns Universitet, juni 2016. Teksten om mødet mellem journalist og forsker på den ene side og bygningen og dens brugere på den anden side. Journalist Christoffer Zieler henviser til Henrik Reehs separat offentliggjo......Reportage fra et fælles besøg i den nyligt indviede PharmaScience Building, Københavns Universitet, juni 2016. Teksten om mødet mellem journalist og forsker på den ene side og bygningen og dens brugere på den anden side. Journalist Christoffer Zieler henviser til Henrik Reehs separat...

  8. Crossfit jako prostředek veslařského tréninku

    OpenAIRE

    Imlauf, Jan

    2017-01-01

    The main topic of this thesis is to map and assess the impact of CrossFit workout on rowers performance. Next is comparison of rowers who practice CrossFit workout and who not, and both fitness and the technical side. In the theoretical part is mentioned characterics of rowing, rowing disciplines, categories, rowing tracks and means of rowers. Other chapters deal with plyometric training, CrossFit and sports training. There are mentioned means of CrossFit workout and exercises suitable for ro...

  9. Pariafemininitetens återuppståndelse : Diskurser om skådespelerskor runt sekelskiftet 1900

    OpenAIRE

    Ohlsson, Hélène

    2016-01-01

    The article discusses the strategies for to go beyond the boundaries of normative femininity in Sweden during the 1890s. The case study is the contemporary popular actress Ellen Hartman’s strategies for a comeback in Stockholm. In 1891, she had created a scandal by breaking her contract with the Royal Dramatic Theater, eloping with a lover, and divorcing her husband. She wanted to remarry, but the plan backfired and she was abandoned. She became a victim of a smearing campaign in the Scandina...

  10. Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80.

    Science.gov (United States)

    Chiewpattanakul, Paramaporn; Phonnok, Sirinet; Durand, Alain; Marie, Emmanuelle; Thanomsub, Benjamas Wongsatayanon

    2010-12-01

    A new biosurfactant producer was isolated from palm-oilcontaminated soil and later identified through morphology and DNA sequencing as the yeast-like fungus Exophiala dermatitidis. Biosurfactant production was catalyzed by vegetable oil, supplemented with a basal medium. The culture conditions that provided the biosurfactant with the highest surface activity were found to be 5% palm oil with 0.08% NH4NO3, at a pH of 5.3, with shaking at 200 rpm, and a temperature of 30 degrees C for a 14-day period of incubation. The biosurfactant was purified, in accordance with surfactant properties, by solvent fractionation using silica gel column chromatography. The chemical structure of the strongest surface-active compound was elucidated through the use of NMR and mass spectroscopy, and noted to be monoolein, which then went on to demonstrate antiproliferative activity against cervical cancer (HeLa) and leukemia (U937) cell lines in a dose-dependent manner. Interestingly, no cytotoxicity was observed with normal cells even when high concentrations were used. Cell and DNA morphological changes, in both cancer cell lines, were observed to be cell shrinkage, membrane blebbling, and DNA fragmentation.

  11. Neeme Jõgi usub Pan-Balti piimanduskontserni rajamisse / Neeme Jõgi ; interv. Viljar Rääsk

    Index Scriptorium Estoniae

    Jõgi, Neeme

    2008-01-01

    Läti piimatööstuse Rigas Piensaimnieksi juhatuse esimees Neeme Jõgi vastab küsimustele, mis puudutavad ettevõtte olukorda, ülebaltikumilise piimafirma tulekut, ärikultuuri erinevusi Lätis, sisulise austuse saavutamist, tema põhimõtteid äritegevuses, vajadust skaneerimisoskustega juhi järele ning Parexiga juhtunu mõju lätlastele. Lisa: Neeme Jõgi

  12. Bohemicalia Zachiana II. Pražské období Jana Zacha

    Czech Academy of Sciences Publication Activity Database

    Slavický, Tomáš

    2002-01-01

    Roč. 39, č. 1 (2002), s. 3-23 ISSN 0018-7003 Institutional research plan: CEZ:AV0Z8059909 Keywords : Jan Zach * Music in Prague in the 18th Century Subject RIV: AL - Art, Architecture, Cultural Heritage

  13. Radar and optical observations and physical modeling of near-Earth Asteroid 10115 (1992 SK)

    Czech Academy of Sciences Publication Activity Database

    Busch, M.; Ostro, S. J.; Benner, L. A. M.; Giorgini, J. D.; Jurgens, R. F.; Rose, R.; Magri, C.; Pravec, Petr; Scheeres, D.J.; Broschart, S.B.

    2006-01-01

    Roč. 181, č. 1 (2006), s. 145-155 ISSN 0019-1035 R&D Projects: GA ČR(CZ) GA205/05/0604; GA AV ČR IAA3003204 Institutional research plan: CEZ:AV0Z10030501 Keywords : asteroids * radar * rotation Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 3.151, year: 2006

  14. Peter Lorange: on aeg hakata uuesti investeerima / Peter Lorange ; intervjueerinud Viljar Rääsk

    Index Scriptorium Estoniae

    Lorange, Peter, 1943-

    2009-01-01

    Šveitsis tegutseva Rahvusvahelise Juhtimiskooli endise juhi hinnangul peaksid valitsused majandusliku languse tingimustes suunama raha infrastruktuuri arendamisse, pakkuma ettevõtjatele maksuvabastusi ja vähendama bürokraatiat

  15. Nirmal Varma o Pražském jaru a srpnu 1968

    Czech Academy of Sciences Publication Activity Database

    Marková, Dagmar

    2008-01-01

    Roč. 63, č. 2 (2008), s. 42-45 ISSN 0029-5302 Institutional research plan: CEZ:AV0Z90210515 Keywords : Nirmal Varma * Prague Spring * Hindi literature Subject RIV: AJ - Letters, Mass-media, Audiovision

  16. Fuzzy modelování hospodářských recesí

    Directory of Open Access Journals (Sweden)

    Vladimíra Kučerová

    2013-11-01

    Full Text Available The paper is a study of a business recessions. On the ground of the actual financial crisis, which has broad international influence, many questions about possibilities of efficient solutions of the economic recessions have been opened. In the economics history is possible to find many examples of business recessions and more or less successful solution methods. Each recession has several analogical characters with other recessions. The similarity of the recessions is utilized in this study as the base for fuzzy model of the business recessions. The fuzzy logic is very useful tool for modeling, because absolute as well as relative quantitative indicators can be used in the model. The fuzzy model of the business recession is based on the data of the American's recessions and also covers the data about the Japanese crisis and one example of the Czech business recession. The model was utilized for research of the recessions' similarities and for the prediction of the aspect of the future business recessions.

  17. Celebrity marketing pražských muzikálů

    OpenAIRE

    Kuzmová, Tereza

    2017-01-01

    This Diploma Thesis deals with the issue of Prague musicals celebrity marketing, which is a significant characteristics of the contemporary Prague musical scene. The theoretical part of this thesis introduces the basic theories of celebrity studies and the specifics of marketing mix of musicals, in which celebrities play a significant role. On the margine, the thesis is also focused on current overproduction of musicals and related problems in marketing activities of musical productions. The ...

  18. Vizualizácia podnikateľského modelu

    OpenAIRE

    Zagorsek, Branislav

    2013-01-01

    Business model describes the company’s most important activities, proposed value, and the compensation for the value. Business model visualization enables to simply and systematically capture and describe the most important components of the business model while the standardization of the concept allows the comparison between companies. There are several possibilities how to visualize the model. The aim of this paper is to describe the options for business model visualization and business mod...

  19. Skąd się wzięła wielkanocna pisanka

    OpenAIRE

    Mędzelowski, Tadeusz

    2016-01-01

    Już w starożytności jajko było przedmiotem kultu. Symbolizowało świat, płodność, zdrowie i szczęście. Uważano, że jest ono początkiem wszystkiego. Olbrzymie jajko miało wyjść z ust najwyższego Boga. Dlatego też zawieszano jajka u sufitu egipskich świątyń. Było też atrybutem egipskiego boga Ptach, który jako garncarz nadaje kształt światu w formie jaja. Podczas pracy bóg - garncarz stłukł jajo i dlatego nastąpił podział świata. W każdym domu starano się mieć jajo, które miało chronić od ...

  20. 2D CFT blocks for the 4D class Sk theories

    International Nuclear Information System (INIS)

    Mitev, Vladimir; Pomoni, Elli

    2017-03-01

    This is the first in a series of papers on the search for the 2D CFT description of a large class of 4D N=1 gauge theories. Here, we identify the 2D CFT symmetry algebra and its representations, namely the conformal blocks of the Virasoro/W-algebra, that underlie the 2D theory and reproduce the Seiberg-Witten curves of the N=1 gauge theories. We find that the blocks corresponding to the SU(N) S k gauge theories involve fields in certain non-unitary representations of the W kN algebra. These conformal blocks give a prediction for the instanton partition functions of the 4D N=1 SCFTs of class S k .

  1. skávání pravidel z dat

    Czech Academy of Sciences Publication Activity Database

    Holeňa, Martin

    2003-01-01

    Roč. 83, č. 2 (2003), s. 48-60 ISSN 0322-788X Institutional research plan: AV0Z1030915 Keywords : rule extraction from data * observational logic * method GUHA * rule extraction with artificial neural networks

  2. Franchisingový koncept mateřské školy

    OpenAIRE

    Matyáštíková, Romana

    2011-01-01

    The thesis is divided into two parts. The first part deals with theoretical description of franchising, we can find there definition, basic terms and types of franchising, advantages and disadvantages for both parties and there is also a description how to build a franchised business, including pilot operation, operational manual or franchise agreement. The second part is dedicated to practical application of franchising on a concept of kindergarten and nursery.

  3. Synergies between the first-generation JHF-SK and NuMI superbeam experiments

    International Nuclear Information System (INIS)

    Huber, P.; Lindner, M.; Winter, W.

    2003-01-01

    We discuss synergies in the combination of the first-generation JHF to Super-Kamiokande and NuMI off-axis superbeam experiments. With synergies we mean effects which go beyond simply adding the statistics of the two experiments. As a first important result, we do not observe interesting synergy effects in the combination of the two experiments as they are planned right now. However, we find that with minor modifications, such as a different NuMI baseline or a partial antineutrino running, one could do much richer physics with both experiments combined. Specifically, we demonstrate that one could, depending on the value of the solar mass squared difference, either measure the sign of the atmospheric mass squared difference or CP violation already with the initial stage experiments. Our main results are presented in a way that can be easily interpreted in terms of the forthcoming KamLAND result

  4. Specifika architektury hronovsko-poříčského zlomu

    Czech Academy of Sciences Publication Activity Database

    Prouza, V.; Coubal, Miroslav; Adamovič, Jiří

    2015-01-01

    Roč. 2014, č. 1 (2015), s. 13-18 ISSN 0514-8057 Institutional support: RVO:67985831 Keywords : fault architecture * fault core * damage zone * drag zone * Hronov-Poříčí Fault Subject RIV: DB - Geology ; Mineralogy

  5. Expression of a SK2-type dehydrin gene from Populus euphratica in ...

    African Journals Online (AJOL)

    Jane

    2011-08-22

    Aug 22, 2011 ... 1Beijing Agro-Biotechnology Research Center, Beijing 100097, China. .... one shoot was isolated from each stem explants to ensure that the transgenic lines were ... (TaKaRa). The first strand of cDNA was used as template to.

  6. 77 FR 27494 - SK Private Investment Fund 1998 LLC, et al.; Notice of Application

    Science.gov (United States)

    2012-05-10

    ... operation, taxation and regulation of Investments and of the Investment Funds. The Investment Committee may... operation and taxation of the Investment Funds. The Administrator may, but is not required to be, a Member... basis. Skadden Arps may in its discretion advance funds to Eligible Investors for the purpose of making...

  7. Uus ärimudel joogi- ja toiduautomaatide turul / Viljar Rääsk

    Index Scriptorium Estoniae

    Rääsk, Viljar

    2008-01-01

    Firma Iberia Delicatessen põhisiht on müügiautomaadid, mille eest nad renti ega hooldustasu ei küsi ning tulu ootavad omanikud ainult toidu ja joogi müügist. Vt. samas: Vendori omanikud rajasid gurmeekohvi äri; Coffee In ei loe kohviautomaate konkurendiks

  8. Podnikateľský zámer - Subway franchise

    OpenAIRE

    Leitner, Tomáš

    2013-01-01

    Subject of this thesis is to create a business plan for the branch of Subway franchise and acquaint with the concept of franchising. Theoretical part of the diploma thesis applies to definition of franchising, its characteristics, features, advantages and disadvantages for both parties and describes the elements necessary for setting up business. In application part of the thesis is introduced the business plan for opening of Subway branch.

  9. Logopedická prevence v mateřské škole

    OpenAIRE

    Velasová, Petra

    2012-01-01

    This bachelor thesis deals with provision of speech prevention in kindergarten. The work includes the problematic of speech and the speech development of children, impact of sensual perception and movement abilities to the speech advancement. The work describes kindergarten and activities that are used in the kindergarten for speech therapy prevention. The aim of this work is to describe methods and forms of speech prevention used in kindergarten. Key words: speech, speech development, langua...

  10. Celodřevěné tesařské spoje

    Czech Academy of Sciences Publication Activity Database

    Hasníková, Hana; Kunecký, Jiří; Fajman, P.

    2015-01-01

    Roč. 9, 1/2 (2015), s. 12-15 ISSN 1802-2030 R&D Projects: GA MK(CZ) DF12P01OVV004 Keywords : all-wooden joints * traditional technology * historical timber structures Subject RIV: AL - Art, Architecture, Cultural Heritage http://www.casopisstavebnictvi.cz/online/

  11. Mizející podmořské pralesy

    Czech Academy of Sciences Publication Activity Database

    Vejřík, Lukáš; Vejříková, Ivana; Peterka, Jiří

    2017-01-01

    Roč. 2017, č. 5 (2017), s. 94-95 ISSN 0373-675X R&D Projects: GA MŠk(CZ) 7F14316 Institutional support: RVO:60077344 Keywords : kelp * macrophytes * Australia Subject RIV: EH - Ecology, Behaviour OBOR OECD: Ecology

  12. René Wellek a Pražská škola

    Czech Academy of Sciences Publication Activity Database

    Sládek, Ondřej

    2016-01-01

    Roč. 1, č. 1 (2016), s. 19-30 ISSN 2453-8507 Institutional support: RVO:68378068 Keywords : literary theory ; structuralism ; poetics ; Wellek, René ; Prague School * structuralism * poetics * Wellek, René * Prague School * Prague linguistic circle * Mukařovský, Jan Subject RIV: AJ - Letters, Mass-media, Audiovision

  13. Archeologie na pražském pracovišti NPÚ

    Czech Academy of Sciences Publication Activity Database

    Sommer, Petr

    2015-01-01

    Roč. 31, č. 2 (2015), s. 115-117 ISSN 0231-6056 Institutional support: RVO:67985912 Keywords : cultural heritage * National Institute * archaeological monuments Subject RIV: AC - Archeology, Anthropology, Ethnology

  14. Endokrynny wpływ tkanki tłuszczowej na stan skóry

    OpenAIRE

    Jaśkiewicz, Jerzy; Goździalska, Anna; Lizak, Dorota

    2012-01-01

    Praca recenzowana / peer-reviewed paper Komórki tkanki tłuszczowej, adipocyty, różnicują się w okresie życia płodowego z pierwotnych fi broblastów. W adipocytach gromadzone są substraty energetyczne w postaci zestryfi - kowanych kwasów tłuszczowych. W komórkach tych zachodzą aktywne procesy syntezy kwasów tłuszczowych, a także reakcji elongacji i desaturacji lipidów. Dla stabilności energetycznej ustroju znaczący wpływ ma korelacja osi insulina–glukagon z aktywnością takich enzymów, jak...

  15. Clinical Characteristics of MuSK Antibody-positive Myasthenia Gravis in Taiwan

    Directory of Open Access Journals (Sweden)

    Yung-Chuan Huang

    2008-07-01

    Full Text Available Circulating antibodies of the acetylcholine receptor (AchRAb are detectable in most patients with generalized myasthenia gravis (MG. A newly discovered antibody against muscle-specific kinase (MuSKAb has been detected in 40–70% of AchRAb-negative MG patients. We report a series of Taiwanese MuSKAb-positive patients, and compare their clinical features with MuSKAb-negative patients and also with MuSKAb-positive Caucasians. Five out of 44 seronegative generalized MG patients (11.4% were positive for MuSKAb. Patients with MuSKAb tended to have severe disability and bulbar involvement, and more often experienced crisis or impending crisis. Although all of these patients showed an initial response to immunosuppressant therapy, they had greater disability at follow-up. The clinical features of Taiwanese MuSKAb-positive patients were not different from those of Caucasians, except for a lower prevalence.

  16. Developing translational medicine professionals : The Marie Skłodowska-Curie action model

    NARCIS (Netherlands)

    Petrelli, Alessandra; Prakken, Berent J.; Rosenblum, Norman D.

    2016-01-01

    End goal of translational medicine is to combine disciplines and expertise to eventually promote improvement of the global healthcare system by delivering effective therapies to individuals and society. Well-trained experts of the translational medicine process endowed with profound knowledge of

  17. Marine bioearosol in the area of Gdańsk Bay

    Directory of Open Access Journals (Sweden)

    Małgorzata Michalska

    2011-03-01

    Full Text Available Formation of bioaerosol is an important process of mass and energy exchange between sea and atmosphere by means of droplets of marine dust. Studies on aerosol activity of the sea was developed in the last decades of the 20 th century. Those studies revealed that concentrations of bacteria in aerosol droplets were hundreds times higher than their concentrations in superficial parts of seawater. The research also proved that aerosol activity of the sea can influence the sanitary condition of the air, especially at the seacoast areas. Surveys of air structure in coastal regions were performed on the Tricity beaches as well as in Sobieszewo and Komary. Atmospheric air sampling stations in the marine zone were located on the Gulf of Gdansk at the Vistula river mouth. Air samples were collected from the front platform on the ship R/V Baltica at 4m above the sea surface. The air samples were collected by filtration method using Sartorius apparatus. Airborne microbes were deposited onto sterile gelatine Sartorius filters. All filters were exposed directly towards the oncoming wind. After sampling, the exposed filters were placed onto the agar media on Petri plates and incubated. All measurement results were depicted in (CFU/m3 i.e. colony forming units in 1 m3 of the examined air. Meteorological measurements included: temperature, humidity, wind speed and direction. The obtained results revealed that statistically significant trends were observed between the total number of bacteria or moulds and a season of sampling. The greatest number of microorganisms was noted in spring and autumn. Correlation analysis showed that statistically significant relationship exists between microbial abundance and the wind direction or speed and location of sampling site. Large quantities of fungal spores was detected in the areas of Gdynia and Gdansk - Brzeêno when south-west winds were blowing from the land. The highest number of bacteria were observed at the sampling stations located closest to the Vistula river mouth (Sobieszewo and Komary.

  18. I konstens namn - scenen som arbetsmiljö : Arbetsplatsmobbning ur skådespelarperspektiv

    OpenAIRE

    Godhwani, Yvonne

    2008-01-01

    Workplace bullying is a topic of current interest in Finland. Workplace bullying is found in all professions, including the artistic ones. This thesis aims to explore workplace bullying from the view of the Finland-Swedish actors as a phenomenon that within dramatic art is difficult to define due to the fact that the body and emotions of an actor constitute his or her working tools. The research aims to deepen the understanding of the actors’ working situation, and particularly of the difficu...

  19. Úplatkářské trestné činy

    OpenAIRE

    Jančík, Marek

    2015-01-01

    This diploma thesis deals with bribery offences which constitute the core of the criminal law prosecution of corruption in our country. Initially, the text discusses the notion of corruption and bribery, then it deals with the development of legislation in this area from 1852 to the present time. The paper also analyses the consequences of changes in the concept of criminal offence under the current Criminal Code (Act No. 40/2009 Coll.) in comparison with the previous one (Act No. 140/1961 Co...

  20. Inclusive K0SK0S resonance production in ep collisions at HERA

    International Nuclear Information System (INIS)

    Chekanov, S.; Derrick, M.; Magill, S.

    2008-06-01

    Inclusive K 0 S K 0 S production in ep collisions at HERA was studied with the ZEUS detector using an integrated luminosity of 0.5 fb -1 . Enhancements in the mass spectrum were observed and are attributed to the production of f 2 (1270)/a 0 2 (1320), f' 2 (1525) and f 0 (1710). The f 0 (1710) state was observed with a statistical significance of 5 standard deviations. Masses and widths were obtained using a fit which takes into account theoretical predictions based on SU(3) symmetry arguments. (orig.)

  1. Begær, forførelse og kvindelig skønhed

    DEFF Research Database (Denmark)

    Hansen-Hansen, Erik

    2008-01-01

    gender-feministiske teoripositioner som er særdeles moralistisk. Da gender-feministisk ideologi i stigende grad bliver en institutionaliseret bureaukratisk magt i de post-industrielle samfund, vil mode blive anset som en hindring af det abstrakte krav om ‘ligestilling’ - forstået som et ideal om...

  2. Novinky pražského mozartovského výzkumu

    Czech Academy of Sciences Publication Activity Database

    Jonášová, Milada

    2016-01-01

    Roč. 24, č. 4 (2016), s. 20-22 ISSN 1210-9525 R&D Projects: GA ČR GAP409/12/2563 Institutional support: RVO:68378076 Keywords : copyists research * autograph * operas Subject RIV: AL - Art, Architecture, Cultural Heritage

  3. Veřejné bruslařské školy

    OpenAIRE

    Vejpravová, Dominika

    2014-01-01

    Title of the thesis: Public skating schools Aim: Mapping of teaching in public skating schools in the selected region. Comparison of methods of teaching skating at these schools on the base of observations and interviews with the coaches. The proposal optimal model of teaching skating suitable for use in public skating schools. The work covers the fundamentals of skating. Key words: Ice - skating, child, school.

  4. Anmeldelser af litteratur om den græsk-romerske oldtid

    DEFF Research Database (Denmark)

    Krasilnikoff, Jens

    2007-01-01

    Anmeldelse af Neville Morley, "Trade in Classical Antiquity". Key Themes in Ancient History. Cambridge UP, 2007. p.22. Anmeldelse af J. Bingen, "Hellenistic Egypt. Monarchy, Society, economy, Culture", Edinburgh UP, 2007, pp.22-23. Anmeldelse af C. Koehn, "Krieg-Diplomatie-Ideologie. Zur Ausenpol......Anmeldelse af Neville Morley, "Trade in Classical Antiquity". Key Themes in Ancient History. Cambridge UP, 2007. p.22. Anmeldelse af J. Bingen, "Hellenistic Egypt. Monarchy, Society, economy, Culture", Edinburgh UP, 2007, pp.22-23. Anmeldelse af C. Koehn, "Krieg-Diplomatie-Ideologie. Zur...... Ausenpolitik hellenistischer Mittelstaaten", Franz Steiner 2007, pp.24-25. Anmeldelse af A. Kühr,"Als Kadmos nach Boiotien kam. Polis und ethnos im Spiegel thebanischer Gründungsmythen", Franz Steiner 2007, p. 25. P. Low, "Interstate relations in Classical Greece. Morality and Power", Cambridge UP 2007, pp. 26...

  5. Nastavovací celodřevěné tesařské spoje

    Czech Academy of Sciences Publication Activity Database

    Kunecký, Jiří; Hasníková, Hana; Fajman, P.; Tippner, J.

    2016-01-01

    Roč. 7, č. 1 (2016), s. 64-66. ISBN 978-80-906132-4-9. ISSN 1803-6996 R&D Projects: GA MK(CZ) DG16P02M026 Keywords : carpentry joints * joint execution * replacement Subject RIV: JN - Civil Engineering

  6. Misjoniseminar Tartus / Rein Kalmus, Janos Kovacs Biro, Rein Käsk...[jt.] ; interv. Mare Pihlak

    Index Scriptorium Estoniae

    2007-01-01

    Vestlus osalejatega Pereraadio saates "Elupuu," mis valmis märtsis Trans-Euroopa Divisjoni väikegruppide ja misjonitöö juhi Janos Kovacs-Biro Tartu Ülikooli Ajaloomuuseumis peetud loengusarja "Ela oma elu täiel määral" põhjal

  7. Marketing Plan of SK Dynamo České Budějovice

    OpenAIRE

    Kramář, Jan

    2012-01-01

    The major goal of this bachelor work is a detailed marketing situational analysis of a current club marketing plan. The marketing plan will be proposed on basis of detailed marketing situational analysis. The club may use the marketing plan as a source for written processing.

  8. Estetická norma artefaktov trácko-skýtskeho horizontu na Slovensku

    Directory of Open Access Journals (Sweden)

    Lukáš Makky

    2012-12-01

    Full Text Available The role of Aesthetics as a science is to examine any expression of aesthetic thinking, evaluate perception and interpretation of created artefacts and analyse options of aesthetic research of the rest of the society and objects commonly on the edge of the interest. Given paper turns the interest on the period of Slovak prehistory (Iron Age when the development of Europe was dictated by the countries of antic Greece and later by antic Roma, specifically about the period when Thracians and Scythians broke into our space. The aim of the paper is an aesthetic interpretation of chosen objects of this period focusing on analysisof aesthetic norm and evaluation of the consequences of its application in practice and on the final form of the objects. It is also connected with aesthetic function, which is necessarily present in objects retaining on aesthetic norm and generally, it could be identified in any objects.

  9. Mezinárodní obchod s jeseterem sibiřským (Acipenser baerii)

    OpenAIRE

    KORUNKOVÁ, Eva

    2015-01-01

    Sturgeon fish (Acipenseriformes) is commercially used to produce high quality fish meat and highly priced caviar. In fact, all species of Sturgeon fish are integrated into CITES appendix. Sturgeon (Acipenser baerii) is the most frequently bred/reared and the most commercial representative of its kind, not only in Europe but also in Asia. The aim of this final project is to evaluate international sturgeon trade while using CITES references from 1998-2012. The source of data(s) was commercial d...

  10. Anmeldelser af litteratur om den græsk-romerske oldtid

    DEFF Research Database (Denmark)

    Krasilnikoff, Jens

    2009-01-01

    Anmeldelser af: S. Cuomo, Technology and Culture in Greek and Roman Antiquity, Cambridge UP, 2007. J. P. Oleson, The Oxford Handbook of Engineering and Technology in the Classical World, Oxford UP, 2008. W. Heckel, The Conquests of alexander the Great, Cambridge 2008. J. D. Lewis, Solon the Think....... Political Thought in Archaic Athens. Duckworth 2008. P. Liddel, Civic Obligation and Indivudual Liberty in Ancient Athens, Oxford UP, 2007 F. Hardy, Revenge in Athenian Culture, Duckworth 2008.   Udgivelsesdato: forår 2009...

  11. Anmeldelser af litteratur om den græsk-romerske oldtid

    DEFF Research Database (Denmark)

    Krasilnikoff, Jens

    2009-01-01

    Anmeldelser af: R. F. Op, Asceticism in the Graeco-Roman world, Cambridge UP, 2009. R. Osborne (ed.), Debating the Athenian Cultural Revolution. Art, Literature, Philosophy, and Politics 430-380 BC. Cambridge UP, 2007. H. Forbes, The Meaning and Identity of a Greek Landscape. An Archaeological...

  12. On the formal equivalence of the TAP and thermodynamic methods in the SK model

    International Nuclear Information System (INIS)

    Cavagna, Andrea; Giardina, Irene; Parisi, Giorgio; Mezard, Marc

    2003-01-01

    We revisit two classic Thouless-Anderson-Palmer (TAP) studies of the Sherrington-Kirkpatrick model (Bray A J and Moore M A 1980 J. Phys. C: Solid State Phys. 13 L469; De Dominicis C and Young A P 1983 J. Phys. A: Math. Gen. 16 2063). By using the Becchi-Rouet-Stora-Tyutin (BRST) supersymmetry, we prove the general equivalence of TAP and replica partition functions, and show that the annealed calculation of the TAP complexity is formally identical to the quenched thermodynamic calculation of the free energy at one step level of replica symmetry breaking. The complexity we obtain by means of the BRST symmetry turns out to be considerably smaller than the previous non-symmetric value

  13. Lesní mateřská školka

    OpenAIRE

    Salák, Martin

    2014-01-01

    In this work is to design wooden house, which will serve as the background of the forest kindergarten, which corresponds to the functional layout and dimensions of the building. The building is designed in accordance with current legislation relating to the building of kindergartens. For the design of this building, were given to natural building materials. The main text contains a description of natural building materials, wall and ceiling tracks, construction of foundations and roof, and al...

  14. Čeština lékařských knih

    Czech Academy of Sciences Publication Activity Database

    Černá, Alena M.

    2015-01-01

    Roč. 52, 1/2 (2015), s. 34-40 ISSN 1212-5326. [Výzkum historické češtiny (na základě nových materiálových bází). Praha, 20.11.2014] R&D Projects: GA ČR GAP406/10/1140 Keywords : Old Czech * medical book * medical terminology Subject RIV: AI - Linguistics http://jazykovednesdruzeni.cz/JA15_12.pdf

  15. Efektivnost koncesionářských poplatků České televize

    OpenAIRE

    Hartmannová, Eva

    2009-01-01

    The work is concerned with the efficiency of the system of financing Czech Televison. Namely, it examines the question whether it is more appropriate to finance public television with concessionary fees or with taxes, considering the fact that at present almost everybody has a tv set. Expenses associated with collecting fees and their recovery, with detection of nonpayers and with maintanance of databases are taken into account. The systems of financing in different European countries are com...

  16. Popularita e-sportu u pražské mládeže

    OpenAIRE

    Inneman, Martin

    2017-01-01

    Title: Popularity of e-sports among Prague's youth Objectives: The main objective of this thesis is to determine popularity of e-sports among Prague's youth and the associated marketing potential of this industry. The thesis also tests the depth of knowledge, so that it is possible to create an overall opinion on the current state of e-sports among Prague's youth. Methods: The method used in this thesis is marketing research. Due to the lack of secondary data it was necessary to obtain primar...

  17. Journal of Earth System Science | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Earth System Science. S Sajani. Articles written in Journal of Earth System Science. Volume 116 Issue 2 April 2007 pp 149-157. The role of low-frequency intraseasonal oscillations in the anomalous Indian summer monsoon rainfall of 2002 · S Sajani S Naseema Beegum K Krishna Moorthy.

  18. Journal of Chemical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences. Wilaiwan Chanmanee. Articles written in Journal of Chemical Sciences. Volume 121 Issue 5 September 2009 pp 655-664. Platinum-carbon black-titanium dioxide nanocomposite electrocatalysts for fuel cell applications · Satheesh Sambandam Vinodh Valluri Wilaiwan ...

  19. Medicinal plants with cholinesterase inhibitory activity: a review

    CSIR Research Space (South Africa)

    Adewusi, EA

    2010-12-01

    Full Text Available al. (2007 a) Mimosa pudica L. Whole plant Water TLC and 96 well plate; 1.68 ? 0.22 (100 ?g/ml) ND Vinutha et al. (2007) Trigonella foenum graecum L. Seeds Hydro alcohol TLC and 96 well plate; 50% (140.26 ?g)* ND SatheeshKumar et al. (2009...

  20. Bulletin of Materials Science | News

    Indian Academy of Sciences (India)

    Mullite retains the usual orthorhombic habit of sillimanite. Rounded to sub rounded zirconia dispersed within the mullite matrix of the sample ZA is noticed. pp 221-225 Sol-gel Materials. Role of binder in the synthesis of titania membrane · K S Seshadri M Selvaraj R Kesava Moorthy K Varatharajan M P Srinivasan K B Lal.

  1. Fulltext PDF

    Indian Academy of Sciences (India)

    R. Narasimhan (Krishtel eMaging) 1461 1996 Oct 15 13:05:22

    D Mukhopadhyay, Roorkee, India. S Mukhopadhya, Roorkee, India. V S N Murty, Goa, India. Ravi S Nanjundiah, Bangalore, India. B M Reddy, Hyderabad, India. Roger Bilham, USA. Sajani Surendran, USA. Sarva Jit Singh, Rohtak, India. S K Satheesh, Bangalore, India. D Sengupta, Bangalore, India. D Shankar, Goa, India.

  2. Fellowship | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Elected: 2010 Section: Earth & Planetary Sciences. Satheesh, Dr Sreedharan Krishnakumari Ph.D.(Kerala), FNA, FNASc, FTWAS. Date of birth: 1 May 1970. Specialization: Aerosols, Radiation, Climate Address: CAOS & Divecha Centre for Climate Change, Indian Institute of Science, Bengaluru 560 012, Karnataka Contact:

  3. Journal of Biosciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Effects of sodium-orthovanadate and Trigonella foenum-graecum seeds on hepatic and renal lipogenic enzymes and lipid profile during alloxan diabetes · Umesh C S Yadav K Moorthy Najma Z Baquer · More Details Abstract Fulltext PDF. Sodium-orthovanadate (SOV) and seed powder of Trigonella foenum graecum Linn.

  4. Journal of Earth System Science | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Earth System Science. Santosh Kumar. Articles written in Journal of Earth System Science. Volume 117 Issue S1 July 2008 pp 303-313. Characteristics of spectral aerosol optical depths over India during ICARB · S Naseema Beegum K Krishna Moorthy Vijayakumar S Nair S Suresh Babu S K ...

  5. Untitled

    Indian Academy of Sciences (India)

    CNDO/force method R Brakaspathy and Surjit Singh 253-260. One-electron reduction of thionine studied by pulse radiolysis. S N Guha, P N Moorthy, K Kishore, D B Naik and K N Rao 261-271. Binding of naproxen to bovine serum albumin and tryptophan-modi- fied bovine serum albumin Meenakshi Maruthanuthu and S ...

  6. Identification of mineral dust layers in high alpine snow packs

    Science.gov (United States)

    Greilinger, Marion; Kau, Daniela; Schauer, Gerhard; Kasper-Giebl, Anne

    2017-04-01

    allow a retrospective evaluation of previous snow chemistry data of snow packs of previous years or different locations. Thus the unique time of almost 30 years of snow chemistry data from glaciers surrounding the Sonnblick observatory [5] can be evaluated, focusing on the intensity and frequency of the occurance of Saharan dust layers in high alpine snow packs. Literature: [1] Boucher, O., Randall, D., Artaxo, P., Bretherton, C., Feingold, G., Forster, P., Kerminen, V.M., Kondo, Y., Liao, H., Lohmann, U., Rasch, P., Satheesh, S.K., Sherwood, S., Stevens, B., Zhang, X.Y., 2013. Clouds and aerosols. In: Stocker, T.F., Qin, D., Plattner, G.-K., Tignor, M., Allen, S.K., Boschung, J., Nauels, A., Xia, Y., Bex, V., Midgley, P.M. (Eds.), Climate Change 2013: the Physical Science Basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA. [2] Prospero, J.M., 1996. Saharan Dust Transport Over the North Atlantic Ocean and Mediterranean: An Overview, in: Guerzoni, S., Chester, R. (Eds.), The Impact of Desert Dust Across the Mediterranean, Environmental Science and Technology Library. Springer Netherlands, pp. 133-151. [3] Avila, A., Rod_a, F., 1991. Red rains as major contributors of nutrients and alkalinity to terrestrial ecosystems at Montseny (NE Spain). Orsis Org. Sist. 6, 215e229. [4] Miles, L.J., Yost, K.J., 1982. Quality analysis of USGS precipitation chemistry data for New York. Atmos. Environ. 1967 (16), 2889e2898. http://dx.doi.org/10.1016/ 0004-6981(82)90039-7. [5] Greilinger, Marion, et al. "Temporal changes of inorganic ion deposition in the seasonal snow cover for the Austrian Alps (1983-2014)." Atmospheric Environment 132 (2016): 141-152.

  7. Rostlinné zbytky ze zaniklé studny. Svědci historie Jiřského náměstí na Pražském hradu ve 13. století

    Czech Academy of Sciences Publication Activity Database

    Čulíková, Věra

    2012-01-01

    Roč. 64, č. 3 (2012), s. 479-502 ISSN 0323-1267 Institutional research plan: CEZ:AV0Z80020508 Keywords : archaeobotany * Middle Ages * Prague Castle * carpological analysis * well Subject RIV: AC - Archeology, Anthropology, Ethnology

  8. Ove Torgny, Hundra procent Roma: en njutbar källa för sköna dagar i Rom (Ängelholm: SkåneFörlaget, 2006

    Directory of Open Access Journals (Sweden)

    Kjartan Ólafsson

    2012-03-01

    Full Text Available Guide books are in many ways a curious type of books. All of them promise to some extent the same promise - to introduce the reader to some place (a town, a country or even a whole continent. In doing so the guide book aims to draw out the essence of that place its true core and presents the reader with the highlights, the ‘best of’. It is somewhat unusual for guide books to spell this out as clearly as is the case with Hundra procent Roma by Ove Torgny. If the title in itself is not clear enough, this promise is also taken up on the back cover which declares that the book picks up the thread where other guide books end.

  9. Stofnanir og nýsköpun á norðurslóðum: Þverfagleg rannsókn á hlutverki sveitarfélaga í nýsköpun

    DEFF Research Database (Denmark)

    Jóhannesson, Gunnar Thor; Hovgaard, Gestur

    2005-01-01

    This article discusses the role of municipalities and their institutions in innovation. It recounts the findings of a multi-national research project on the matter within the Nordic periphery and focuses on findings from the Faroe Islands and Iceland. Innovation is a term often emphasized...

  10. Pastva hospodářských zvířat a její využití v ochranářské praxi

    Czech Academy of Sciences Publication Activity Database

    Hejcman, M.; Pavlů, V.; Krahulec, František

    2002-01-01

    Roč. 37, - (2002), s. 203-216 ISSN 0032-7786 R&D Projects: GA AV ČR KSK6005114 Institutional research plan: CEZ:AV0Z6005908 Keywords : nature conservation * mown meadows * grazing Subject RIV: EF - Botanics

  11. Pääsküla raamatukogu : Pärnu mnt. 480A, Tallinn = Pääsküla Library : Pärnu mnt. 480A, Tallinn / Triin Ojari

    Index Scriptorium Estoniae

    Ojari, Triin, 1974-

    2005-01-01

    Kahekorruselise lehisega vooderdatud raamatukoguhoone üks külg on klaasist. Projekteerija: Arhitektid Muru & Pere. Autorid: Urmas Muru, Peeter Pere. Konstruktor Ago-Allan Kuddu. Sisekujundus: Katrin Kaevats, Urmas Muru, Peeter Pere. Projekt: 2002-2003, valmis: 2005. Ill.:4 värv. välis ja 2 sisevaadet, I ja II korruse plaan

  12. Pyloanalytické výsledky ze zaniklé studny Jiřského náměstí Pražského hradu

    Czech Academy of Sciences Publication Activity Database

    Jankovská, Vlasta

    2012-01-01

    Roč. 64, č. 3 (2012), s. 503-507 ISSN 0323-1267 Institutional support: RVO:67985939 Keywords : pollen analysis and non-pollen-palynomorphs analysis * Prague castle * Middle Ages Subject RIV: EF - Botanics

  13. Renesanční podoba Pražského hradu z archeologického pohledu

    Czech Academy of Sciences Publication Activity Database

    Blažková, Gabriela

    2015-01-01

    Roč. 7, č. 2 (2015), s. 121-134 ISSN 1804-1132. [Prag - Residenz des Habsburgers Ferdinand I. 1526-1564. Praha, 26.03.2015-28.03.2015] R&D Projects: GA ČR GP13-34374P Institutional support: RVO:67985912 Keywords : Prague * Prague Castle * Renaissance * ceramic * glass Subject RIV: AC - Archeology, Anthropology, Ethnology

  14. skávání a výběr pracovníků

    OpenAIRE

    Bláhová, Lucie

    2008-01-01

    Recruitment and staff selection is a topic which deserves attention to be given in literature. Recruitment and staff selection are areas of personnel work that are crucial for any organization because they influence its efficiency. The fundamental part of successful selection process is job analysis which is followed by recruitment. Every organization should consider choosing recruiting from internal (inner) sources or recruiting from external (outer) sources, or its combination. Another issu...

  15. Faktory ovlivňující hodnocení atraktivity mužské postavy

    OpenAIRE

    Třebický, Vít

    2012-01-01

    According to previous research physical attractiveness plays an important role in our everyday life. People are treated differently on the basis of their physical appearance and from an evolutionary point of view physical attractiveness is a key factor in mate selection, being a cue of an individual's mate value and genetic qualities. Research shows that the highest attractiveness ratings tend to be given to physiques with a higher level of development of lean muscle mass and a V shaped upper...

  16. DuSK: A Dual Structure-preserving Kernel for Supervised Tensor Learning with Applications to Neuroimages

    Science.gov (United States)

    He, Lifang; Kong, Xiangnan; Yu, Philip S.; Ragin, Ann B.; Hao, Zhifeng; Yang, Xiaowei

    2015-01-01

    With advances in data collection technologies, tensor data is assuming increasing prominence in many applications and the problem of supervised tensor learning has emerged as a topic of critical significance in the data mining and machine learning community. Conventional methods for supervised tensor learning mainly focus on learning kernels by flattening the tensor into vectors or matrices, however structural information within the tensors will be lost. In this paper, we introduce a new scheme to design structure-preserving kernels for supervised tensor learning. Specifically, we demonstrate how to leverage the naturally available structure within the tensorial representation to encode prior knowledge in the kernel. We proposed a tensor kernel that can preserve tensor structures based upon dual-tensorial mapping. The dual-tensorial mapping function can map each tensor instance in the input space to another tensor in the feature space while preserving the tensorial structure. Theoretically, our approach is an extension of the conventional kernels in the vector space to tensor space. We applied our novel kernel in conjunction with SVM to real-world tensor classification problems including brain fMRI classification for three different diseases (i.e., Alzheimer's disease, ADHD and brain damage by HIV). Extensive empirical studies demonstrate that our proposed approach can effectively boost tensor classification performances, particularly with small sample sizes. PMID:25927014

  17. Marketingová strategie lyžařské školy School 4 fun.

    OpenAIRE

    Burešová, Eva

    2011-01-01

    The aim of diploma thesis is to suggest new marketing strategy of ski and snowboard school SCHOOL 4 FUN for this year 2011/2012 including proposals and recommendation for its improvement. Suggested marketing strategy is the solution for suitable position of limited financial means for the company to maintain competitive advantage, increase its sale, thus increase its revenue at the same time. To achieve given aim, the technique of competitive analysis, marketing mix and SWOT analysis was used...

  18. Measuring the Social Impact of Infrastructure Projects: The Case of Gdańsk International Fair Co.

    Directory of Open Access Journals (Sweden)

    Anna Zamojska

    2017-01-01

    Full Text Available Efcient infrastructure is a prerequisite of, and critcal to, development. Only some projects generate a positve rate of return, but all of them should generate positve non-economic impacts and contribute social gains. Social impact is considered as a consequence or efect of decisions or interventons which lead to development. It can also be considered as a social consequence of development. The main problem of social costs and benefts is that the impact is difcult to predict and quantfy and can be taken into account diferently by authorites, decision makers and project developers. The main purpose of the paper is to identfy and demonstrate a concept of the social impact of infrastructure projects. The principal methods used are a review of existng social science literature and surveys based on focus group interviews, devoted stakeholders of infrastructure projects, and their involvement at diferent stages of the project. The expected result is a set of outputs and outcomes which demonstrates social impacts (costs and benefts related to stakeholders’ groups of the analyzed project.

  19. DuSK: A Dual Structure-preserving Kernel for Supervised Tensor Learning with Applications to Neuroimages.

    Science.gov (United States)

    He, Lifang; Kong, Xiangnan; Yu, Philip S; Ragin, Ann B; Hao, Zhifeng; Yang, Xiaowei

    With advances in data collection technologies, tensor data is assuming increasing prominence in many applications and the problem of supervised tensor learning has emerged as a topic of critical significance in the data mining and machine learning community. Conventional methods for supervised tensor learning mainly focus on learning kernels by flattening the tensor into vectors or matrices, however structural information within the tensors will be lost. In this paper, we introduce a new scheme to design structure-preserving kernels for supervised tensor learning. Specifically, we demonstrate how to leverage the naturally available structure within the tensorial representation to encode prior knowledge in the kernel. We proposed a tensor kernel that can preserve tensor structures based upon dual-tensorial mapping. The dual-tensorial mapping function can map each tensor instance in the input space to another tensor in the feature space while preserving the tensorial structure. Theoretically, our approach is an extension of the conventional kernels in the vector space to tensor space. We applied our novel kernel in conjunction with SVM to real-world tensor classification problems including brain fMRI classification for three different diseases ( i.e ., Alzheimer's disease, ADHD and brain damage by HIV). Extensive empirical studies demonstrate that our proposed approach can effectively boost tensor classification performances, particularly with small sample sizes.

  20. Duchenne Müsküler Distrofi ve Gilbert"s Sendromu Birlikteliği: Bir Olgu Sunumu

    OpenAIRE

    İncecik, Faruk; Hergüner, Özlem M.; Mert, Gülen; Horoz, Özden; Altunbaşak, Şakir

    2013-01-01

    Gilbert"s syndrome is characterized by unconjugated hyperbilirubinemia. A 5-year-old boy presented to our hospital with mild hyperbilirubinemia. The patient had persistent unconjugated hyperbilirubinemia with high liver enzymes and creatine phosphokinase. Haemolysis was excluded by normal haemoglobin, and reticulocyte count and finally he was diagnosed to have Gilbert"s syndrome. His creatine kinase concentration was 15600 U/l, and he had a deletion in the dystrophin gene. Finally, the patien...

  1. Duchenne Müsküler Distrofi ve Gilbert"s Sendromu Birlikteliği: Bir Olgu Sunumu

    OpenAIRE

    İncecik, Faruk; Hergüner, Özlem M.; Mert, Gülen; Horoz, Özden; Altunbaşak, Şakir

    2014-01-01

    Gilbert"s sendromu konjuge olmayan hiperbilirubinemi ile karekterize bir hastalıktır. 5 yaşında erkek çocuğu hafif sarılık ile hastanemize getirildi. Hastanın karaciğer enzimleri ve kreatin fosfokinaz yüksekliği ile beraber ısrarcı unkonjuge hiperbilirubinemisi vardı. Normal hemoglobin ve retikülosit değerleri ile hemoliz dışlandı ve Gilbert"s sendromu tanısı konuldu. Kreatin kinaz değeri 15600 U/l idi ve distrofin geninde delesyon mevcuttu. Sonuç olarak hastaya Gilbert"s sendr...

  2. MiR-200a enhances the migrations of A549 and SK-MES-1 cells by ...

    Indian Academy of Sciences (India)

    2013-07-14

    Jul 14, 2013 ... The lack of treatment options for patients with meta- .... The relative levels of mature miR-200a in different lung ... Mutant derivatives with mutations of 5 bases (at .... future work, because metastatic phenotype may ascribe to.

  3. Joris Karl Huysmans jako kritik umění a jeho pražské odezvy

    Directory of Open Access Journals (Sweden)

    Tomáš Kolich

    2017-09-01

    Generally speaking, Huysmans’s reviews were widely read in the Czech Lands, although their selection was limited. Czech art periodicals found his reviews interesting for his daring style and a broad range of themes, which also included little-known artists, caricatures and posters.

  4. Vliv světové hospodářské krize na export ČR

    OpenAIRE

    Hastík, Jan

    2010-01-01

    The submitted master's thesis analyzes the impact of the global economic crisis on the Czech economy and export of the Czech republic. On the basis of statistical data thesis examines the evolution of basic macroeconomic indicators in the period before the crisis and during an economic downturn, which characterizes the current economy situation in the Czech republic. The work depicts the changes in the state support for exports, the government taken measures and shortcomings in our export pol...

  5. Pojem významu ve světle pražské poetiky a estetiky

    Czech Academy of Sciences Publication Activity Database

    Kubínová, Marie

    2005-01-01

    Roč. 66, č. 2 (2005), s. 98-102 ISSN 0037-7031 R&D Projects: GA ČR GA405/03/0416 Institutional research plan: CEZ:AV0Z90560517 Keywords : semiotics * structuralism Subject RIV: AJ - Letters, Mass-media, Audiovision

  6. Brenda D. Phillips, Deborah S.K. Thomas, Alice Fothergill, Lynn Blinn‑Pike (orgs., Social Vulnerability to Disasters.

    Directory of Open Access Journals (Sweden)

    Susana Freiria

    2012-10-01

    Full Text Available Actualmente registam‑se em todo o mundo um número cada vez mais elevado de desastres e cujo impacto na vida das populações e nos seus bens é cada vez maior. Num cenário de catástrofe é importante conhecer os recursos humanos disponíveis, os cenários possíveis, os grupos mais vulneráveis em relação aos diversos perigos e quais as suas principais fragilidades. O livro Social Vulnerability to Disasters apresenta‑se como inovador na medida em que alia o conhecimento académico desenvolvido no âmb...

  7. Paleontologický výzkum v permokarbonu podkrkonošské pánve

    Czech Academy of Sciences Publication Activity Database

    Šimůnek, Z.; Stárková, M.; Zajíc, Jaroslav; Mikuláš, Radek; Drábková, J.

    2010-01-01

    Roč. 2009, - (2010), s. 167-171 ISSN 0514-8057 Institutional research plan: CEZ:AV0Z30130516 Keywords : Krkonoše-Piedmont Basin * Carboniferous * Permian * fauna * flora * palynomorphs * ichnofossils Subject RIV: DB - Geology ; Mineralogy http://www.geology.cz/zpravy/obsah/2009/2009-45.pdf

  8. Forenzní šetření a hospodářská kriminalita

    OpenAIRE

    Švajnochová, Gabriela

    2016-01-01

    Topic of this master thesis is forensic investigation and forms of economic crime which can be detected during the investigation. First chapter focuses on forensic investigation and its comparison with statutory audit. In the second chapter is performed analysis of forms of economic crime in the Czech Republic, Southern and Eastern Europe and globally. Knowledge from the previous chapters is applied to the real forensic investigation performed in the Czech Republic, forensic investigation of ...

  9. Modernizace hudebně pohybové výchovy v mateřské škole

    OpenAIRE

    Kubaň, Antonín

    2010-01-01

    Abstract Title: Innovation of music-based physical education activities for pre-school kids Objectives: The aim of the thesis is to draft and attempt to validate the comprehensive interventional educational programme for pre-school kids focused on all-around personal development. Side effect of the executed research was the support of pre-school kid's natural and comprehensive kinetic development, sensorimotor, musical and rhytmical competences with the stress on personal health and hygiene (...

  10. Mediální výchova v mateřské škole

    OpenAIRE

    PAPEŽOVÁ, Petra

    2013-01-01

    Today's modern society use mass media on a daily basis as a modern way of communication. Lot of us can't imagine everyday life without them. They are surrendering us with non-stop flow of various informations. Everyday we spent a lot of time with media and sometimes we even don't realize, what risks are menacing us and others. Media are capable of manipulating with people without even noticing it by common population. They are influencing their opinions, behavior and thinking. Kids and teen-a...

  11. Pražské setkání národnostních menšin

    Czech Academy of Sciences Publication Activity Database

    Štědroň, J.; Sulitka, Andrej

    -, č. 3 (2014), s. 14 ISSN 1210-9525 Institutional support: RVO:68378076 Keywords : national minorities * minorities organizations * Prague * coexistence of minorities * Annual Conference Subject RIV: AC - Archeology, Anthropology, Ethnology

  12. Z historie výuky fyziky pro mediky na pražských univerzitách

    Czech Academy of Sciences Publication Activity Database

    Těšínská, Emilie; Hlaváčková, Ludmila

    2012-01-01

    Roč. 51, č. 1 (2012), s. 11-57 ISSN 0323-0562 Institutional research plan: CEZ:AV0Z80630520 Keywords : history of physics * history of medicine * history of the Charles University Subject RIV: AB - History

  13. skávání pracovníků do Call centra RWE

    OpenAIRE

    Popovičová, Jasmína

    2016-01-01

    The aim of the bachelors thesis is to analyse the recruitment process at Call centre RWE, explore untapped labour market, identify strengths and weaknesses of the recruitment process and make suggestions to improve current labour situation. Thesis is divided into two sections - theoretical and practical. Theoretical section describes terms and theory of recruitment. Practical section consists of the description of the company, current situation in recruitment at the Call centre RWE, results o...

  14. Podnikatelský plán výstavby lázeňského domu

    OpenAIRE

    Eisenreich, Jindřich

    2015-01-01

    The purpose of this dissertation is a formulation and analysis of a business plan for development of a new spa resort in Františkových Lázních and conduct feasibility study of proposed business case. The following work is divided into theoretical and practical part. While theoretical section of the dissertation outlines main terms and theoretical concepts, explains relevant legal and regulatory conditions and restrictions relating to offering spa services and details all factors necessary for...

  15. Implementace logistických technologií v potravinářském průmyslu

    OpenAIRE

    ŽEMLIČKA, Kamil

    2014-01-01

    This diploma thesis focuses on proposal of possibilities when applying logistic technologies in food supply chain of the MADETA, a.s. The thesis describes material and information flow in the supply chain, including determination of key factors for successful implementation of chosen technologies. A part of the thesis deals with the proposal for a change in the way of preparing orders for customers of independent market, including stating of expected costs and savings when realizing the propo...

  16. MiR-200a enhances the migrations of A549 and SK-MES-1 cells by ...

    Indian Academy of Sciences (India)

    By a series of gain-of-function and loss-offunction studies, over-expression of miR-200a was indicated to enhance cells migration, and its knock-down inhibited migration of cells in NSCLC cell lines. Furthermore, miR-200a was identified to induce TSPAN1 expression which was related to migration. TSPAN1 was proved to ...

  17. Undersøgelse af pulsformers indflydelse på piercing og skæring i rummet

    DEFF Research Database (Denmark)

    Horstmann, Birgitte D.; Juhl, Thomas Winther

    1999-01-01

    Der er gennem en række laboratorieforsøg gennemført piercing og hjørneskæring i rummet (3D) i AISI 316 stål. Det er blevet konstateret at der kan opnås piercingtider på ned til ca. 1 sekund i det anvendte 5 mm pladestål. Tre forskellige pulsformer, Gated puls, Hyper puls og Super puls er blevet...

  18. sk number üks - saavutada täiskontroll - on täidetud / Toomas Hendrik Ilves

    Index Scriptorium Estoniae

    Ilves, Toomas Hendrik, 1953-

    2006-01-01

    Raamatuarvustus: Baker, Peter; Glasser, Susan. Kremlin Rising: Vladimir Putin's Russia and the End of Revolution. New York: Scribner, 2005. Raamatu autorid on USA ajalehe Washington Post Moskva korrespondendid. Arvustus ilmunud ka: Sirp : Diplomaatia 7. okt. 2005 nr. 25, lk. 14-15

  19. Metodika pro rozvoj techniky bruslení na bruslařském trenažeru

    OpenAIRE

    Soukup, Jan

    2016-01-01

    Title: Methodology for development of skating technique on a skatemill Goals: The objective of the present study is to compile available methodology knowledge re-garding the improvement of skating technique using a skatemill, and to analyse the pro-gress of skating technique in selected ice-hockey players during a comprehensive train-ing programme, where a skating simulator is used. Keywords: Ice Hockey, skatemill, kinematic analysis, hockey skills, motoric skills, ska-ting, modern aids. Powe...

  20. Využití projektové metody v mateřské škole

    OpenAIRE

    KODIMOVÁ, Zuzana

    2012-01-01

    This bachelor's thesis revolves around using planning method, which enhances life in many nursery schools. The theoretical part is aimed at its characteristics, origin and history; it informs us about signs and structure of the project and draws attention about the advantages and disadvantages of the method. In the functional part, research survey is carried out, which examines current notion of educators on the subject of applying the planning method using surveys and self-observation to ass...

  1. Možnosti využití čerpadel ve vinařských provozech

    OpenAIRE

    Blaha, Tomáš

    2015-01-01

    This bachelor thesis discusses pumps that are commonly used in viniculture. The characteristic of working conditions shows the environment in which these devices work, what their basic technical parameters are, and which substances contained in wine influence their lifespan. The characteristic of transported material lists materials that can be found in viniculture. According to the type of transportation, design solutions of the pumps are distinguished. Two alternatives of wine production - ...

  2. Formalizace lékařských doporučení pro nestabilní anginu pectoris

    Czech Academy of Sciences Publication Activity Database

    Peleška, Jan; Anger, Z.; Aschermann, M.; Tomečková, Marie; Veselý, Arnošt; Zvárová, Jana

    2003-01-01

    Roč. 45, 4 suppl. (2003), s. 72 ISSN 0010-8650. [Výroční sjezd České kardiologické společnosti /11./. 11.05.2003-14.05.2003, Brno] R&D Projects: GA MŠk LN00B107 Keywords : electronic medical guidelines Subject RIV: BD - Theory of Information

  3. Funkce peněz v teorii hospodářského cyklu

    OpenAIRE

    Smejkal, Tomáš

    2009-01-01

    This bachelor thesis is dealing with the topic money and especially emphasizing its function in business cycles. An ambition of this thesis is to resolve dilemma if money, as an important instrument in the real economy, can influence the fluctuation of economy. This thesis is based on the comparison of two distinct schools of economic thought- Post Keynesian and Austrian school. Both approaches despite different opinion on creation and function of money refuse the theory of money neutrality. ...

  4. Vykazování hráčských smluv ve fotbale dle IFRS

    OpenAIRE

    Kučera, Martin

    2016-01-01

    The goals of this diploma thesis are to characterize the current stadium of disclosure information about player contracts in football based on the IFRS standards and describe possible policy improvements for accounting standards. Theoretical part focus on an analysis of chosen IFRS standards, especially IAS 38 and UEFA regulations. Practical part of the thesis present a research of player contracts in the hands of particular football clubs with an emphasis on the method of acquisition.

  5. Neznámý Dvořák ze staré skříně

    Czech Academy of Sciences Publication Activity Database

    Kachlík, Jan; Kratochvílová, Markéta

    2014-01-01

    Roč. 2014, č. 11 (2014), s. 18-19 ISSN 1210-9525 Institutional support: RVO:68378076 Keywords : Antonín Dvořák * opera * Alfred * original version Subject RIV: AL - Art, Architecture, Cultural Heritage http://abicko.avcr.cz/2014/11/07/dvorak.html

  6. Branding potravinářských výrobků na trhu ČR

    OpenAIRE

    Průša, Přemysl

    2004-01-01

    The aim of this thesis is to summarize and develop a theoretical framework for brand management, which could be usable in the business practice, and apply this framework on a reality of a chosen brand in the Czech foodstuffs - wine market. In the analytical part the aim is to analyze the current status of the chosen wine brand including its current way of brand management and further develop a new system of brand management of the chosen brand using the framework developed in the theoretical ...

  7. OLAIFA A. Kl, ONWUKA S.K2*, ARIYIBI A3 AND CRAIG 0. 0.1

    African Journals Online (AJOL)

    Femi Olaleye

    small animal interference even though it has become less fashionable in countries that have facilities for inhalation anaesthesia (Hall and Clarke, 1983). It is marketed in this country as a sterile 6.5%. (W /V) solution containing propylene glycol and as a powder in gelatine capsules. For euthanasia it is sold as 20% (W /V).

  8. Study of the K$^{0}_{s}$K$^{0}_{s}$ final state in two-photon collisions

    CERN Document Server

    Acciarri, M; Adriani, O; Aguilar-Benítez, M; Ahlen, S P; Alpat, B; Alcaraz, J; Allaby, James V; Aloisio, A; Alverson, G; Alviggi, M G; Ambrosi, G; An, Q; Anderhub, H; Andreev, V P; Angelescu, T; Antreasyan, D; Arefev, A; Azemoon, T; Aziz, T; Baba, P V K S; Bagnaia, P; Baksay, L; Ball, R C; Banerjee, S; Banicz, K; Barillère, R; Barone, L; Bartalini, P; Baschirotto, A; Basile, M; Battiston, R; Bay, A; Becattini, F; Becker, U; Behner, F; Bencze, G L; Berdugo, J; Berges, P; Bertucci, B; Betev, B L; Biasini, M; Biland, A; Bilei, G M; Bizzarri, R; Blaising, J J; Böck, R K; Böhm, A; Borgia, B; Boucham, A; Bourilkov, D; Bourquin, Maurice; Boutigny, D; Braccini, S; Brambilla, Elena; Branson, J G; Brigljevic, V; Brock, I C; Buijs, A; Bujak, A T; Burger, J D; Burger, W J; Burgos, C; Busenitz, J K; Buytenhuijs, A O; Cai, X D; Capell, M; Cara Romeo, G; Caria, M; Carlino, G; Cartacci, A M; Casaus, J; Castellini, G; Castello, R; Cavallari, F; Cavallo, N; Cecchi, C; Cerrada-Canales, M; Cesaroni, F; Chamizo-Llatas, M; Chan, A; Chang, Y H; Chaturvedi, U K; Chemarin, M; Chen, A; Chen, C; Chen, G; Chen, G M; Chen, H F; Chen, H S; Chen, M; Chiefari, G; Chien, C Y; Choi, M T; Cifarelli, Luisa; Cindolo, F; Civinini, C; Clare, I; Clare, R; Coan, T E; Cohn, H O; Coignet, G; Colijn, A P; Colino, N; Commichau, V; Costantini, S; Cotorobai, F; de la Cruz, B; Cui, X T; Cui, X Y; Dai, T S; D'Alessandro, R; De Asmundis, R; De Boeck, H; Degré, A; Deiters, K; Dénes, E; Denes, P; De Notaristefani, F; DiBitonto, Daryl; Diemoz, M; Van Dierendonck, D N; Di Lodovico, F; Dionisi, C; Dittmar, Michael; Dominguez, A; Doria, A; Dorne, I; Dova, M T; Drago, E; Duchesneau, D; Duinker, P; Durán, I; Dutta, S; Easo, S; Efremenko, Yu V; El-Mamouni, H; Engler, A; Eppling, F J; Erné, F C; Ernenwein, J P; Extermann, Pierre; Fabbretti, R; Fabre, M; Faccini, R; Falciano, S; Favara, A; Fay, J; Felcini, Marta; Ferguson, T; Fernández, D; Fernández, G; Ferroni, F; Fesefeldt, H S; Fiandrini, E; Field, J H; Filthaut, Frank; Fisher, P H; Forconi, G; Fredj, L; Freudenreich, Klaus; Gailloud, M; Galaktionov, Yu; Ganguli, S N; García-Abia, P; Gau, S S; Gentile, S; Gerald, J; Gheordanescu, N; Giagu, S; Goldfarb, S; Goldstein, J; Gong, Z F; González, E; Gougas, Andreas; Goujon, D; Gratta, Giorgio; Grünewald, M W; Gu, C; Guanziroli, M; Gupta, V K; Gurtu, A; Gustafson, H R; Gutay, L J; Hartmann, B; Hasan, A; He, J T; Hebbeker, T; Hervé, A; Hilgers, K; Van Hoek, W C; Hofer, H; Hoorani, H; Hou, S R; Hu, G; Ilyas, M M; Innocente, Vincenzo; Janssen, H; Jin, B N; Jones, L W; de Jong, P; Josa-Mutuberria, I; Kasser, A; Khan, R A; Kamyshkov, Yu A; Kapinos, P; Kapustinsky, J S; Karyotakis, Yu; Kaur, M; Khokhar, S; Kienzle-Focacci, M N; Kim, D; Kim, J K; Kim, S C; Kim, Y G; Kinnison, W W; Kirkby, A; Kirkby, D; Kirkby, Jasper; Kittel, E W; Klimentov, A; König, A C; Koffeman, E; Kornadt, O; Koutsenko, V F; Koulbardis, A; Krämer, R W; Kramer, T; Krenz, W; Kuijten, H; Kunin, A; Ladrón de Guevara, P; Landi, G; Lapoint, C; Lassila-Perini, K M; Laurikainen, P; Lebeau, M; Lebedev, A; Lebrun, P; Lecomte, P; Lecoq, P; Le Coultre, P; Lee Jae Sik; Lee, K Y; Leggett, C; Le Goff, J M; Leiste, R; Lenti, M; Leonardi, E; Levchenko, P M; Li Chuan; Lieb, E H; Lin, W T; Linde, Frank L; Lindemann, B; Lista, L; Liu, Y; Liu, Z A; Lohmann, W; Longo, E; Lu, W; Lü, Y S; Lübelsmeyer, K; Luci, C; Luckey, D; Ludovici, L; Luminari, L; Lustermann, W; Ma Wen Gan; Macchiolo, A; Maity, M; Malgeri, L; Malik, R; Malinin, A; Maña, C; Mangla, S; Maolinbay, M; Marchesini, P A; Marin, A; Martin, J P; Marzano, F; Massaro, G G G; Mazumdar, K; McNally, D; Mele, S; Merola, L; Meschini, M; Metzger, W J; Mi, Y; Mihul, A; Van Mil, A J W; Mir, Y; Mirabelli, G; Mnich, J; Möller, M; Monteleoni, B; Moore, R; Morand, R; Morganti, S; Moulai, N E; Mount, R; Müller, S; Muheim, F; Nagy, E; Nahn, S; Napolitano, M; Nessi-Tedaldi, F; Newman, H; Niaz, M A; Nippe, A; Nowak, H; Organtini, G; Ostonen, R; Pandoulas, D; Paoletti, S; Paolucci, P; Pascale, G; Passaleva, G; Patricelli, S; Paul, T; Pauluzzi, M; Paus, C; Pauss, Felicitas; Pei, Y J; Pensotti, S; Perret-Gallix, D; Petrak, S; Pevsner, A; Piccolo, D; Pieri, M; Pinto, J C; Piroué, P A; Pistolesi, E; Plyaskin, V; Pohl, M; Pozhidaev, V; Postema, H; Produit, N; Qureshi, K N; Raghavan, R; Rahal-Callot, G; Rancoita, P G; Rattaggi, M; Raven, G; Razis, P A; Read, K; Redaelli, M; Ren, D; Ren, Z; Rescigno, M; Reucroft, S; Ricker, A; Riemann, S; Riemers, B C; Riles, K; Rind, O; Rizvi, H A; Ro, S; Robohm, A; Rodin, J; Rodríguez-Calonge, F J; Roe, B P; Röhner, S; Romero, L; Rosier-Lees, S; Rosselet, P; Van Rossum, W; Roth, S; Rubio, Juan Antonio; Rykaczewski, H; Salicio, J; Salicio, J M; Sánchez, E; Santocchia, A; Sarakinos, M E; Sarkar, S; Sartorelli, G; Sassowsky, M; Sauvage, G; Schäfer, C; Shchegelskii, V; Schmitz, D; Schmitz, P; Schneegans, M; Schöneich, B; Scholz, N; Schopper, Herwig Franz; Schotanus, D J; Schulte, R; Schultze, K; Schwenke, J; Schwering, G; Sciacca, C; Sehgal, R; Seiler, P G; Sens, Johannes C; Servoli, L; Shevchenko, S; Shivarov, N; Shoutko, V; Shukla, J; Shumilov, E; Son, D; Sopczak, André; Soulimov, V; Smith, B; Spickermann, T; Spillantini, P; Steuer, M; Stickland, D P; Sticozzi, F; Stone, H; Stoyanov, B; Strauch, K; Sudhakar, K; Sultanov, G G; Sun, L Z; Susinno, G F; Suter, H; Swain, J D; Tang, X W; Tauscher, Ludwig; Taylor, L; Timellini, R; Ting, Samuel C C; Ting, S M; Toker, O; Tonisch, F; Tonutti, M; Tonwar, S C; Tóth, J; Tsaregorodtsev, A Yu; Tsipolitis, G; Tully, C; Tuchscherer, H; Ulbricht, J; Urbàn, L; Uwer, U; Valente, E; Van de Walle, R T; Vetlitskii, I; Viertel, Gert M; Vikas, P; Vikas, U; Vivargent, M; Völkert, R; Vogel, H; Vogt, H; Vorobev, I; Vorobyov, A A; Vuilleumier, L; Wadhwa, M; Wallraff, W; Wang, J C; Wang, X L; Wang, Y F; Wang, Z M; Weber, A; Weill, R; Willmott, C; Wittgenstein, F; Wu, S X; Wynhoff, S; Xu, J; Xu, Z Z; Yang, B Z; Yang, C G; Yang, G; Yao, X Y; Ye, C H; Ye, J B; Ye, Q; Yeh, S C; You, J M; Yunus, N; Zaccardelli, C; Zalite, A; Zemp, P; Zeng, J Y; Zeng, M; Zeng, Y; Zhang, Z; Zhang, Z P; Zhou, B; Zhou, G J; Zhou, J F; Zhou, Y; Zhu, G Y; Zhu, R Y; Zichichi, Antonino; Van der Zwaan, B C C

    1995-01-01

    The reaction \\epem\\ra\\epem\\gamma^*\\gamma^*\\ra\\epem\\kos\\kos is studied with the L3 detector at LEP and the formation of the f_2\\, \\!\\!\\!'(1525) resonance is observed. For an integrated luminosity of 114 \\pb, % \\ collected over the years 1991-1994, 31\\pm6 f_2\\, \\!\\!\\!' events are found. Their angular distribution is consistent with a pure helicity two. The radiative width times the branching ratio is measured to be \\Gamma_{\\gamma\\gamma}(f_2\\,\\!\\!\\!')\\times Br(f_2\\ \\!\\!\\!'\\ra \\k\\bar{\\k})=(0.093\\pm 0.018\\pm 0.022) keV. Only three events are found in the f_2(1270)-a_2(1320) mass region, consistent with destructive f_2-a_2 interference in the \\kos\\kos \\ final state. The mixing angle of the tensor meson nonet is determined to be \\theta=(29.4^{+1.4}_{-1.6})^\\circ.

  9. Pařížská univerzita a husité v letech 1428-1429

    Czech Academy of Sciences Publication Activity Database

    Coufal, Dušan

    2015-01-01

    Roč. 18, č. 1 (2015), s. 205-235 ISSN 0862-979X R&D Projects: GA ČR(CZ) GBP405/12/G148 Institutional support: RVO:67985955 Keywords : The University of Paris * Hussites * propaganda * The Council of Basel * Nicholas of Clemanges * John of Ragusa Subject RIV: AB - History

  10. Návrh vhodných strategií pro potravinářský podnik

    OpenAIRE

    Homolka, Ondřej

    2014-01-01

    Homolka, O. Design of appropriate strategies for an food company. Bachelor thesis. Brno: Mendel University, 2014. This bachelor thesis deals with evaluation of the current situation and proposal of appropriate strategies for the food company for the future. The thesis is divided to two main parts. The theoretical part is compiled from technical literature. The practical part is based on the analyses of external and internal business environment on which are identified opportunities and threat...

  11. Kosík, Kalivoda, Sviták a Pražské jaro 1968

    Czech Academy of Sciences Publication Activity Database

    Mervart, Jan

    2012-01-01

    Roč. 60, Suppl. 2 (2012), s. 195-209 ISSN 0015-1831 Institutional support: RVO:67985955 Keywords : Marxist humanism * Prague spring 1968 * Karel Kosík * Robert Kalivoda * Ivan Sviták Subject RIV: AA - Philosophy ; Religion

  12. Philipp Weselsky - profesor vídeňské techniky z Českomoravské vysočiny

    Czech Academy of Sciences Publication Activity Database

    Jindra, Jiří

    2010-01-01

    Roč. 43, č. 4 (2010), s. 255-263 ISSN 0300-4414 Institutional research plan: CEZ:AV0Z80630520 Keywords : Vienna Technical University 1854-1884 * tuition of analytical chemistry * synthetic dyes Subject RIV: AB - History

  13. Podnikateľský zámer študentskej reštaurácie/baru Jumper

    OpenAIRE

    Pitoňáková, Anna

    2017-01-01

    My title of bachelor´s thesis is the business plan for the student restaurant/bar, which I would like to open in my home town Poprad. The aim of this thesis is creating a business plan, based on which I will analyze if it is realistic to open a restaurant in my chosen place. My work will be divided into two separate parts. The first part will focus on the theory of entrepreneurship and business plan. In the second part, I will describe how I imagine the restaurant and bar I want to open. Then...

  14. Podnikateľský plán na založenie reštaurácie

    OpenAIRE

    Pandošová, Petronela

    2009-01-01

    The aim of this thesis was to create a business plan that will help in achieving the objectives in the gastronomy field. The theoretical part describes the various parts of the business plan.Practical section describes a specific business plan for the Family Restaurant in Humenné.

  15. Mutation of the cyclic di-GMP phosphodiesterase gene in Burkholderia lata SK875 attenuates virulence and enhances biofilm formation.

    Science.gov (United States)

    Jung, Hae-In; Kim, Yun-Jung; Lee, Yun-Jung; Lee, Hee-Soo; Lee, Jung-Kee; Kim, Soo-Ki

    2017-10-01

    Burkholderia sp. is a gram-negative bacterium that commonly exists in the environment, and can cause diseases in plants, animals, and humans. Here, a transposon mutant library of a Burkholderia lata isolate from a pig with swine respiratory disease in Korea was screened for strains showing attenuated virulence in Caenorhabditis elegans. One such mutant was obtained, and the Tn5 insertion junction was mapped to rpfR, a gene encoding a cyclic di-GMP phosphodiesterase that functions as a receptor. Mutation of rpfR caused a reduction in growth on CPG agar and swimming motility as well as a rough colony morphology on Congo red agar. TLC analysis showed reduced AHL secretion, which was in agreement with the results from plate-based and bioluminescence assays. The mutant strain produced significantly more biofilm detected by crystal violet staining than the parent strain. SEM of the mutant strain clearly showed that the overproduced biofilm contained a filamentous structure. These results suggest that the cyclic di-GMP phosphodiesterase RpfR plays an important role in quorum sensing modulation of the bacterial virulence and biofilm formation.

  16. Živý slon a pražské dvorské slavnosti v roce 1570

    Czech Academy of Sciences Publication Activity Database

    Šárovcová, Martina

    2014-01-01

    Roč. 59, 1/2 (2014), s. 34-40, 43 ISSN 0036-5351 Institutional support: RVO:68378033 Keywords : elephant * Gradual of Sixtus of Ottersdorf * illuminated manuscripts * literati brotherhood at the church of Our Lady before Týn in Prague * court festivities * exotic animals in Renaissance Europe Subject RIV: AL - Art, Architecture, Cultural Heritage

  17. Interplay between dynamic screened f-f interaction and sk·sf interaction in Ce systems

    International Nuclear Information System (INIS)

    Lopez-Aguilar, F.; Costa-Quintana, J.; Sanchez-Lopez, M.M.

    1997-01-01

    Two complementary analyses for the electronic structure of Ce systems are given. The first is performed from the renormalized density of states (DOS) deduced from the interacting Green close-quote s functions. These Green close-quote s functions are obtained from a self-energy calculated from a multiband Hubbard Hamiltonian and using the random phase approximation to account for the dynamical f-f screened interactions. The resulting DOS presents agreements and discrepancies with the spectral data yielded by direct and inverse photoemission. The theoretical and experimental f widths next to the Fermi level imply f-electron masses that are in strong contradiction with those obtained from the heavy-fermion specific heat. We have carried out a second analysis that complements the first one, since it considers the spin-exchange between extended states and a spin-field that is completely excluded from the first calculation. By the marriage of the results obtained in the first calculation with those of the second analysis, we can relate the photoemission spectra and de Haas endash van Alphen masses with the measurements of the contribution of the low-energy quasiparticles to the specific heat and the magnetic susceptibility. copyright 1997 The American Physical Society

  18. Blockade of IP[subscript 3]-Mediated SK Channel Signaling in the Rat Medial Prefrontal Cortex Improves Spatial Working Memory

    Science.gov (United States)

    Brennan, Avis R.; Dolinsky, Beth; Vu, Mai-Anh T.; Stanley, Marion; Yeckel, Mark F.; Arnsten, Amy F. T.

    2008-01-01

    Planning and directing thought and behavior require the working memory (WM) functions of prefrontal cortex. WM is compromised by stress, which activates phosphatidylinositol (PI)-mediated IP[subscript 3]-PKC intracellular signaling. PKC overactivation impairs WM operations and in vitro studies indicate that IP[subscript 3] receptor (IP[subscript…

  19. Vliv lázeňské léčby na obezitu dětí

    OpenAIRE

    CHMELAŘOVÁ, Jana

    2011-01-01

    This baccalaureate work is bent on influence bath treatment on obesity children with a view to for - set in childish medical institution in Poděbrady. Theoretic part deal with excess luggage plus obesity man with more detailed focus on children plus adolescent. In experimental parts was effected anthropometric metering at the beginning plus at the end medical cure in phase October 2010 as far as February 2011. Experimental cope take part in 39 for - set of both sexes in 5- 6 weekly medical cu...

  20. Podnikatelský plán - soukromá mateřská škola

    OpenAIRE

    Boráňová, Andrea

    2010-01-01

    This final thesis is regarding the creating and evaluation of the business plan of private nursery school. The theoretical part is about small and medium-sized enterprises and business plan itself. Practical part contains concrete business plan. Crucial parts of the business plan are: analysis of the environment, marketing mix, financial plan, risk analysis.

  1. Antibodies to AChR, MuSK and VGKC in a patient with myasthenia gravis and Morvan's syndrome.

    Science.gov (United States)

    Díaz-Manera, Jordi; Rojas-García, Ricard; Gallardo, Eduard; Juárez, Cándido; Martínez-Domeño, Alejandro; Martínez-Ramírez, Sergi; Dalmau, Josep; Blesa, Rafael; Illa, Isabel

    2007-07-01

    A 46-year-old woman presented to a local hospital with acute respiratory failure and a 2-year progressive history of fatigue, personality changes, increased sweating, dysphagia with substantial weight loss, dysarthria, and intermittent ptosis and diplopia. Neurological examination showed facial weakness, lingual atrophy and bulbar palsy, which necessitated the use of a feeding tube and ventilatory support. Mild limb weakness with severe muscle atrophy and diffuse muscle twitches were observed. The patient had also developed visual hallucinations and persecutory delusions. Her personal and family medical histories were unremarkable. Sensory and motor nerve conduction studies, repetitive nerve stimulation, electromyogram, blood-cell counts, general chemistry and metabolic function tests, a CT scan, an [(18)F]fluorodeoxyglucose-PET scan, and tests for serum antibodies to acetylcholine receptors, muscle-specific tyrosine kinase, voltage-gated potassium channels, P/Q-type voltage-gated calcium channels, and paraneoplastic antigens, were carried out. Myasthenia gravis associated with antibodies to acetylcholine receptor and muscle-specific tyrosine kinase, and Morvan's syndrome associated with antibodies to voltage-gated potassium channels in the absence of thymoma. Combined treatment with prednisone, intravenous immunoglobulin, ciclosporin, and rituximab.

  2. Biologie, výskyt a regulace kostivalu lékařského(Symphytum officinale L.)

    OpenAIRE

    KOVÁŘOVÁ, Jaroslava

    2014-01-01

    The bacherol thesis with the title "Biology, incidence and regulation of common comfrey (Symphytum officinale L.)" deals with processing and presentation of accessible information about the weed common comfrey. First part of the bacherol thesis paid attention to information related to weed species general characteristics, occurrence and potential of their regulation. Prevailing part of the bacherol thesis occupied with the common comfrey characteristic and regulation possibilities. Informatio...

  3. Foundations of Effective Influence Operations: A Framework for Enhancing Army Capabilities

    Science.gov (United States)

    2009-01-01

    most useful analogues for understanding how marketing principles can benefit influence operations. Case Study of Influence in advertising and Marketing ...1998, pp. 167–197. Kotler , Philip, Marketing Management: Analysis, Planning, Implementation, and Control, 8th ed., Englewood Cliffs, N.J.: Prentice...York: W. W. Norton & Co., 1961. Lilien, Gary L., Philip Kotler , and K. Sridhar Moorthy, Marketing Models, Englewood Cliffs, N.J.: Prentice Hall, 1992

  4. Hospodářský výsledek a jeho transformace na daňový základ

    OpenAIRE

    KUTÍLKOVÁ, Aneta

    2008-01-01

    Operating results and its transformation to tax base is one of essential accounting operations in the end of the accounting period. Operating results is in economy seen as overall title for profit or loss, which a company records during a certain period. To be able to achieve the operating results, we have to go through a sequence of operations leading to the finishing of the accounting period. These operations are called Final accounts and we divide it further into advance working, closing o...

  5. D IE M IST IEK — 'N B E SK O U IN G V O L G E N S D IE ST A N D ...

    African Journals Online (AJOL)

    Test

    tariaat ge!d, die absolute waarheid moet wees. ,Dié waarheid is vir. Berdjajew onafhank!ik, dit word gedien, is abso!uut en dien nie. Hy het 'n vaste ge!oof in die persona!istiese aristokrasie van die gees. Vanuit Rus!and is hy na Duitsland. Met die fascisme en Nazi- isme kon Berdjajew egter ook nie volkome tereg kom nie.

  6. Výchova ke zdravému životnímu stylu v mateřské škole

    OpenAIRE

    ŠINDLEROVÁ, Silvie

    2015-01-01

    This thesis deals with the way the motivation of preschool children by parents and kindergarten teachers . The theoretical part explains the basic concepts of the topic , also characterizes the specifics of the program healthy kindergarten . Another part is focused on risk aspects affecting child health. The practical part is to look at the views of parents and teachers on education for a healthy lifestyle and to compare these views with teachers and parents from the standard program and a pr...

  7. Barokní palác Colloredo-Mansfeldů na Starém Městě pražském

    Czech Academy of Sciences Publication Activity Database

    Vlček, Pavel

    2014-01-01

    Roč. 30, č. 1 (2014), s. 2-24 ISSN 0231-6056 Institutional support: RVO:68378033 Keywords : Prague * high Baroque * Rococo * Giovanni Battista Alliprandi * Colloredo-Mansfeld palace Subject RIV: AL - Art, Architecture, Cultural Heritage

  8. Analýza vinařské turistiky a agroturistiky v regionu Jižní Morava

    OpenAIRE

    Harmáčková, Alena

    2010-01-01

    The bachelor thesis analyses a supply of wine tourism and agro tourism in the South Moravian region. The main goal of the paper is an evaluation of present-day condition of these types of tourism in the region and a comparison with the Weinviertel region.

  9. Daňové a zdravotnické výzvy hospodářské politiky : tři eseje

    OpenAIRE

    Chalupka, Radovan

    2010-01-01

    The first paper evaluates tax legislation in Slovakia, effected January 2004 and major changes in the period 2005 - 2010, according to principles of optimal taxation and optimal tax systems. The author evaluates the Slovak system with particular regard to taxes on labour, taxes on capital and taxes on consumption. The adoption of a flat personal-income-tax rate and a uniform VAT rate is viewed as in line with the optimal taxation theory, and the projected lessening of administrative costs and...

  10. Mötet mellan skäggiga män : Hur ett socialt möte skapar en plats

    OpenAIRE

    Werbowsky, Charlotte

    2016-01-01

    ABSTRACT Today our physical appearance is more important than before. The way we physically present ourselves is how many of us would like to be seen. By acting a certain way, dressing a certain way or spend time with a certain social group we identify ourselves. A certain style that an individual presents can say something about what that individual are interested in and how the person spends his or her time. Today many complement their physical appearance with a physical attribute like a be...

  11. Hospodářská krize a její dopady na automobilový průmysl USA

    OpenAIRE

    Kouřil, Jakub

    2015-01-01

    Bachelor thesis is engaged in an impacts of economic crisis on automotive indus-try in the United States of America. A purpose of thesis is analyse impacts of mortgage and financial crisis on automotive industry and each manufacturers of automobiles in the U.S. First part of thesis is briefly engaged in origins, process and consequences of mortgage and financial crisis. Further thesis describes initial analysis of automotive industry in the United States and the greatest automobile manufactur...

  12. Nepůvodní druhy sladkovodních ryb středomořského úmoří Balkánu

    OpenAIRE

    Balog, Štefan

    2016-01-01

    Mediterranean basin of the Balkan Peninsula is one of the hot-spots of biodiversity, with a high diversity of freshwater fishes and high degree of endemism. Introduction of non-native species greatly affects ecosystems and native species of fish. There were many reasons for introductions in the past. Aquaculture, sport fishing and ornamental fish belong to the most important reasons for introduction of non-native fish species. Altogether, 39 species belonging to 12 families were introduced in...

  13. Hudební události pražského kláštera alžbětinek v roce 1776

    Czech Academy of Sciences Publication Activity Database

    Michl, Jakub

    2016-01-01

    Roč. 8, 1-2 (2016), s. 130-148 ISSN 1803-7828 Institutional support: RVO:68378076 Keywords : Sisters of Saint Elizabeth * cantatas * oratorios * Rochus Elinger SJ * Emilián Rickert OCist. * Marie Deodata a Presentatione B. V. Mariae OSE (Anna Justina von Klausniz) * Marie Juliana a Septem SS. Patribus OSE * Prague * music in convents Subject RIV: AL - Art, Architecture, Cultural Heritage

  14. Změna hodnoty firmy v průběhu hospodářského cyklu

    OpenAIRE

    Táborská, Veronika

    2010-01-01

    The diploma thesis is focused on influence of the economic environment to the business value. The goal of the thesis is a corporate valuation of the entity to 31.12.2009 in recent time of unstable market and financial crisic and how is the business value changeable depenging on business cycle. At the beginning summary of theoretical data concerning business cycle theories is introduced, followed by a description of business cycle and GDP of the Czech Republic from 1996 to 2010. Key part of th...

  15. The effect of VMP35 supplement ingredients encapsulated in a novel Phospholipid Prodosome SK713 SLP nutrient delivery technology on properties of live human blood.

    OpenAIRE

    Kenneth Blum; B. William Downs; Steven Kushner; Ted Aloisio; Frans J. Cronjé

    2015-01-01

    Background: In North America digestive malfunction in terms of disintegration, dissolution, and absorption of food and nutrients, is a widespread malady. Malabsorption is also an exacerbating factor in most chronic degenerative diseases that might benefit from dietary supplementation. The purpose of this experiment was to determine, as shown by changes in properties of live blood, whether, a novel soy-lecithin-phospholipid-nutrient encapsulation technology could promote rapid bioavailability ...

  16. Možnosti získávání informací o uživatelích internetu

    OpenAIRE

    Ross, Richard

    2011-01-01

    This thesis is aimed on internet security, gaining the anonymity and creating a guide to increase self defense of internet users. The goal of this paper is to describe the types of cyber attacks that can be done on internet users and to describe circular flow of information about users on internet and its use by third parties. Additional goal is to design the ways of reaching the anonymity on internet and to provide a guide to increase a self defense of the users. This thesis gradually descri...

  17. Wytrzymałość na ścinanie odpadów komunalnych a stateczność skarp składowisk

    OpenAIRE

    Tymoteusz Zydroń; Mariusz Cholewa; Piotr Demczuk

    2015-01-01

    W pierwszej części pracy dokonano analizy literatury tematycznej dotyczącej parametrów charakteryzujących wytrzymałość na ścinanie odpadów komunalnych. Wykazała ona, że odpady komunalne charakteryzują się bardzo zróżnicowanymi warto­ściami kąta tarcia wewnętrznego i spójności, które zależą m.in. od ich struktury, stopnia rozkładu, metody badań, w tym zakresu odkształceń. Wpływ tych czynników na wytrzyma­łość na ścinanie odpadów jest często niejednoznaczny, co powoduje, że dobór...

  18. Pařížská léta Otty Mizery. Několik drobných poznámek

    Czech Academy of Sciences Publication Activity Database

    Košnarová, Veronika

    2014-01-01

    Roč. 62, č. 5 (2014), s. 774-784 ISSN 0009-0468 Institutional support: RVO:68378068 Keywords : Mizera, Otta * Paris * Parallèle 50 * Tribuna * Czech writers Subject RIV: AJ - Letters, Mass-media, Audiovision

  19. Řivnačské sídliště v Úholičkách, okr. Praha-západ

    Czech Academy of Sciences Publication Activity Database

    Dobeš, Miroslav; Vojtěchovská, I.

    2008-01-01

    Roč. 60, č. 2 (2008), s. 261-297 ISSN 0323-1267 Institutional research plan: CEZ:AV0Z80020508 Keywords : Eneolithic * settlement * Řivnáč culture * Central Bohemia Subject RIV: AC - Archeology, Anthropology, Ethnology

  20. Návrat žen po mateřské a rodičovské dovolené na trh práce

    OpenAIRE

    VALTROVÁ, Olga

    2011-01-01

    The Bachelor?s thesis deals with the problems of women returning to the labour market after a maternity and parental leave. The theoretical part of the thesis describes the situation of Czech women on the labour market and the instruments to fit together family and professional life of women in the Czech Republic. It analyzes the level of employment of women in the Czech Republic, how women are put at a disadvantage on the labour market as a result of gender stereotypes and describes the inst...

  1. Špitální kostel sv. Ducha v Krnově a jeho malířská výzdoba

    Czech Academy of Sciences Publication Activity Database

    Všetečková, Zuzana

    2010-01-01

    Roč. 59, č. 1 (2010), s. 21-54 ISSN 1211-3131 Institutional research plan: CEZ:AV0Z80330511 Keywords : Krnov, Hl. Spirit Church (CZ) * medieval mural paintings * iconography of Old Testament * scenes of Last Supper * New Testament scenes * Legend of St. Peter * after 1450 Subject RIV: AL - Art, Architecture, Cultural Heritage

  2. Pharmacologic inhibition of small-conductance calcium-activated potassium (SK) channels by NS8593 reveals atrial antiarrhythmic potential in horses

    DEFF Research Database (Denmark)

    Haugaard, Maria Mathilde; Hesselkilde, Eva Zander; Pehrson, Steen Michael

    2015-01-01

    and ventricular cardiomyocytes and contribute to atrial repolarization. Inhibition by NS8593 terminates pacing-induced AF of short duration and decreases AF duration and vulnerability without affecting ventricular conduction and repolarization. Thus, inhibition by NS8593 demonstrates clear atrial antiarrhythmic...

  3. Antropologický rozbor kosterních pozůstatků z polozemnice kultury řivnáčské z Kolína

    Czech Academy of Sciences Publication Activity Database

    Brzobohatá, Hana

    2015-01-01

    Roč. 19, č. 2 (2015), s. 543-546 ISSN 1214-3553 Institutional support: RVO:67985912 Keywords : anthropological analysis * Eneolithic * ulnar fracture Subject RIV: AC - Archeology, Anthropology, Ethnology

  4. Pohřebiště Pražského hradu a možnosti jejich sociální interpretace

    Czech Academy of Sciences Publication Activity Database

    Frolík, Jan; Kaupová, S.

    2016-01-01

    Roč. 41, č. 1 (2016), s. 101-122 ISSN 0231-5823 R&D Projects: GA ČR GB14-36938G Institutional support: RVO:67985912 Keywords : burial ground * Prague Castle * Early Middle Ages * elite * stable isotopes * social differentiation * nutrition Subject RIV: AC - Archeology, Anthropology, Ethnology

  5. Oslabuje a zabíjí nás hospodářský růst?

    OpenAIRE

    Ságner, Petr

    2012-01-01

    The thesis analyzes the impact of the business cycle (represented by unemployment rate) on health (represented by mortality rates). Regression analysis has been done on panel data for 20 European countries from 2000 to 2007. Using fixed effects method with inclusion of robust errors, confirms a statistically significant effect of business cycle on 3 specific mortality rates. I reveal, that increase in growth rate of unemployment by 1% point is associated with 1,4% decrease in growth rate of m...

  6. O vydávání Bible gdaňské v 18. a 19. století

    Czech Academy of Sciences Publication Activity Database

    Pišna, Jan

    -, č. 2017 (2017), s. 66-79. ISBN 978-80-89303-56-4. ISSN 1337-0723 Institutional support: RVO:68378068 Keywords : history of book culture, biblical editions, Bible in Polish, Evangelical church in Poland, 17 th– 20th century * biblical editions * Bible in Polish * Evangelical church in Poland * 17th– 20th century Subject RIV: AJ - Letters, Mass-media, Audiovision OBOR OECD: Specific literatures

  7. Prosociální dovednosti jako součást etické výchovy v mateřské škole

    OpenAIRE

    Nixová, Helena

    2016-01-01

    Preschool age is one of the most important periods. What a child learns and what he/she experiences at the preschool age has an impact on all his life. I see a big problem in that today some children cannot cooperate together, they do not share things, do not automatically help the younger and weaker, they cannot solve any conflicts and do not have basic social habits such as greetings, thanks and apology. Therefore it was my goal to include pro-social activities into the kindergarten environ...

  8. V cizích službách. Život a smrt pražského fundatisty

    Czech Academy of Sciences Publication Activity Database

    Freemanová, Michaela

    2012-01-01

    Roč. 31, - (2012), s. 317-327 ISSN 0231-7443 Institutional support: RVO:68378076 Keywords : Brothers Hospitallers' Hospital * boy Choristers * Prague * 18th Century * boy s and young men Subject RIV: AL - Art, Architecture, Cultural Heritage

  9. Nutričně cenné látky v pekařských výrobcích

    OpenAIRE

    Stará, Markéta

    2016-01-01

    The bachelor thesis "Nutritive valuable substances in bakery products" firstly describes important nutrition substances, which may occur in bakery products such as complex carbohydrates, fibre, antioxidants and many more important substances. In the following parts author deals with: chemic composition of alternative crops used in bakery production, various types of seeds (mostly flax seeds, sunflower seeds and sesame seeds), which may be used for enriching products and composition of product...

  10. Hydrokraté a česká vodohospodářská mise ve dvacátém století (1930-1960)

    Czech Academy of Sciences Publication Activity Database

    Janáč, Jiří

    2017-01-01

    Roč. 24, 1/2 (2017), s. 19-53 ISSN 1210-7050 R&D Projects: GA ČR GA15-04902S Institutional support: RVO:68378114 Keywords : Czechoslovakia * water management * planning Subject RIV: AB - History OBOR OECD: History ( history of science and technology to be 6.3, history of specific sciences to be under the respective headings)

  11. Analýza spotřebního chování klientů lázeňského hotelu

    OpenAIRE

    Veselá, Zuzana

    2009-01-01

    The diploma paper is in early part mainly focus on marketing, marketing research and possibility of marketing development in the field. During theoretical part thesis is working with specific problems of marketing in spa and hotel industry. Following chapter is talking mainly about charakteristic of specific spa hotel, its desciption, effects of environment, marketing strategy and competition analysis. Point of primary resaearch is analysis of customer satisfaciton survey during years 2008-20...

  12. Právní režim ochrany biodiverzity mořského dna za hranicemi národní jurisdikce

    OpenAIRE

    Majovská, Barbara

    2017-01-01

    The seabed has long been an unexplored area and we still do not have all the information about its environment. In the second half of the 20th century, the development of technology allowed a better exploration of the seabed. There have been discovered seamounts, hydrothermal vents and other formations. Around these formations there are rich ecosystems that are currently threatened by mining, deep-sea fishing, bioprospecting and deep-sea tourism. Most of the seabed is beyond the area of natio...

  13. Podnikatelský plán – zprostředkování kuchařských kurzů

    OpenAIRE

    Marková, Kristýna

    2015-01-01

    Business plan is an important document any start-up, who is standing in front of a decision of realisation a new business idea. The main goal of this master thesis is complex business plan and evaluation of profitability and attractively of CookForMe, a platform where can people contact yourself and order home cooking lessons by not professional chef on the Czech market. The thesis is divided into two sections, theoretical and practical. Theoretical part presents necessary information for the...

  14. Det institutionelle skøn på rehabiliteringsområdet for børn med handicap og deres forældre

    DEFF Research Database (Denmark)

    Bonderup, Mette

    2013-01-01

    there is a risk that the organizational context may cause social workers to transfer focus from the child´s needs to the question whether other professionals might be able meet the demand. Or it might also be a point of focus whether the parents of children with disabilities already receive support in one form...... discourse and its impact on the child's disability. The analysis also suggests that the organizational context through specific tools e.g. forms and questionnaires is closely associated with The Service Act. Thus both the Multiple Disabilities Understanding of The Social Service Act as well as the dominant...... of the institution in which she is employed. A dual positioning which makes the social worker redefine the child's need of support and transfer the responsibility for support to other professionals. At the same time, the analysis indicates that the social worker chooses her position as an employee...

  15. Falls in elderly people: a multifactorial analysis of risk markers using data from the Swedish general population study 'Good ageing in Skåne'.

    Science.gov (United States)

    Stenhagen, Magnus; Nordell, Eva; Elmståhl, Sölve

    2013-04-01

    The aim of this study was to describe the prevalence of falls in a general older population, especially among the most elderly, and the risk markers associated with falls. This is a cross-sectional study in which 38 fall risk markers were analysed in non-, occasional- and frequent-fallers. The population was 2,865 individuals (aged 60-93), randomly selected from the general population register. The risk of falling was calculated as age-adjusted odds ratios. The relation between the number of risk markers for an individual and falls was also analysed. About one in ten reported falling during the past 6 months, 35% of which were over 90 years old. Twenty-one risk markers were significantly related to falls confirming falling as a multifactorial problem. These included a variety of diseases, symptoms, medical and physical functions, life-style factors and the taking of certain drugs. The five risk markers with the highest odds ratio in frequent fallers were 'tendency to fall' (37.9), 'low walking speed' (12.8), consumption of 'neuroleptics' (10.9), 'impaired mobility' (10.0) and 'dementia' (5.4). Subjects with more than four and seven risk markers showed a 9- respectively 28-fold increase in the risk of falling, especially among frequent fallers and those aged over 90 years. Falls are common in the elderly population and the risk is multifactorial. The results imply that there is an overrepresentation of fallers in a distinct subgroup of the very elderly and those with multiple risk markers. The self-perceived clinical sign 'tendency to fall' seems highly sensitive as indicator of individuals at risk. Several risk markers may be treatable. Fall risk seems to increase in a non-linear, almost exponential way with increasing number of risk markers.

  16. Zabezpečení žen na mateřské a rodičovské dovolené

    OpenAIRE

    Baboráková, Renáta

    2017-01-01

    This thesis focuses on the welfare system of women during maternity and parental leave, primarily for specific benefits, which are in maternity and parenthood provided. System of state social support to women subject to conditions childbirth, child benefits and parental pay. For the childbirth, the work highlights specific situations that may arise in life. They are taking a child into permanent parental care, surrogate motherhood and the death of a child. For child benefits is given consider...

  17. Fričova pařížská léta tučná i hubená

    Czech Academy of Sciences Publication Activity Database

    Hesová, Petra

    2016-01-01

    Roč. 61, 1-2 (2016), s. 63-72 ISSN 0036-5351 Institutional support: RVO:68378068 Keywords : Frič, Josef Václav Frič * Literary Archives of the Museum of Czech Literatur * personal library * National Museum Library * literary history * 19th century Subject RIV: AJ - Letters, Mass-media, Audiovision

  18. Marketingový význam ženských a mužských rolí

    OpenAIRE

    Stříteský, Václav

    2007-01-01

    This thesis deals with gender roles and their importance for marketing on consumer markets. This thesis is based on the general premise that gender roles are dynamic, adaptive, and there may be trends of convergence. Since gender roles are ranked among cultural and social predispositions of consumers, their knowledge can be regarded as one of the major prerequisites of successful marketing orientation on consumer markets. This thesis deals with the differences of gender roles in terms of cons...

  19. Postavy, novinářské texty a jejich emblémy (v psaní Terezy Boučkové)

    Czech Academy of Sciences Publication Activity Database

    Kudlová, Klára

    3 /20/, č. 2 (2011), s. 96-111 ISSN 1337-9275 Institutional research plan: CEZ:AV0Z90560517 Keywords : Czech literature * "authentic" fiction * emblem * Boučková, Tereza * palimpsest * hypertextual discourse Subject RIV: AJ - Letters, Mass-media, Audiovision

  20. K nálezu padělku pražského groše na hradě Kozlov (Chlum)

    Czech Academy of Sciences Publication Activity Database

    Durdík, Tomáš

    10, - (2006), s. 335-336 ISSN 1211-6831 R&D Projects: GA MK(CZ) DB06P01OPP004 Institutional research plan: CEZ:AV0Z80020508 Keywords : castle * castellology * Kozlov (Chlum) * coin falsification * medieval archeology * middle age s * Bohemia * numismatics Subject RIV: AC - Archeology, Anthropology, Ethnology

  1. Prubířský kámen raně středověké společnosti

    Czech Academy of Sciences Publication Activity Database

    Ježek, Martin

    2012-01-01

    Roč. 64, č. 1 (2012), s. 26-58 ISSN 0323-1267 Institutional research plan: CEZ:AV0Z80020508 Keywords : touchstone * Middle Ages * exchange * burial * metallurgy * hoard * precious metal * elite * social stratification * whetstone Subject RIV: AC - Archeology, Anthropology, Ethnology

  2. Důsledky světové hospodářské krize pro Československo ve 30. letech 20. století

    OpenAIRE

    PAŘIL, Aleš

    2013-01-01

    This Bachelor's work deals with the consequences of the Great Depression for Czechoslovakia in the 1930's. To better understand the situation before the Great Depression the work begins with characteristic of Czechoslovakia in the 1920's. The course of this crisis is also shortly outlined there. This bachelor's work focuses primarily on the consequences of the Great Depression on the Czechoslovakian economy connected with the social security of the inhabitants. In this work we can also read a...

  3. Management inovací v Plzeňském kraji a srovnání se sousedními bavorskými regiony

    Directory of Open Access Journals (Sweden)

    Jaroslav Dokoupil

    2017-12-01

    Full Text Available Innovations are key factor for developing of the regions and their competitiveness. There are significant regional discrepancies in the Pilsen region, with specific regions along the border with Bavaria. On one hand, there is economically strong core area around the Pilsen agglomeration, and on the other hand, on contrary with this fact, there are poor borderland regions. The result of those regional discrepancies, having their roots in past, is weakening of the regional cohesion that finally leads to impairing of its competitiveness in terms of current integration process within the EU. Ascertainment of the innovation potential of the Pilsen region and comparing its competitiveness with neighboring Bavarian regions should be a fundamental material that can help to seek tools for efficient regional development of borderland regions. Their innovation potential is expected to be lower in comparison with the core regions, their competitive advantage is, however, their geographical position. They are located near to richer Bavarian regions and thus influences of the borders effects are expected. Diffusion effect and effect of potentially different could positively influence the situation in the Czech borderland. That’s why the authors also measured the innovation potential in neighboring Bavarian region. The goal of this territorial analysis was the socio-economic structure of targeted area, regional discrepancies (in terms of demography, education and employment, innovation potential of local businesses, research and development, and how those issues are interconnected with commercial sector. Results of the research are maps of indexes of innovations in sub regions. It is obvious, that there is a strong concentration of innovative activities to Pilsen and there are different approaches to innovation in the Pilsen region in comparison with Bavaria. The institutions focused on innovation infrastructure within the Pilsen region are mostly located into Pilsen. If there is a stakeholder interested in innovation outside of Pilsen, information innovation centers (which are only a few, though refer this stakeholder into Pilsen. Innovation information centers in Bavaria are, on the other hand, very dispersed. These centers are closer to the stakeholders of regional development and they are active in disseminating of innovation effort and possibilities in their target area. If we look at the EU globally, we can see that Bavarian regions belong to top innovative regions, however the Pilsen region (the part of NUTS2 Jihozapad belongs to group of “slightly innovating members”. Based on the research, the Pilsen region could be recommended to focus its attention more to regional innovative centers.

  4. Obchod potravinářskými výrobky mezi ČR a Francií

    OpenAIRE

    Šembera, Martin

    2010-01-01

    This thesis aims to analyse trade of food products between France and Czech republic. The first chapter features french food industry. The second chapter analyses evolution and trends in import of french food products and possibilities for chzech food on french market. The third part prospects potential of importation of cider to Czech republic.

  5. Effect of post-myocardial infarction streptokinase (sk) therapy, on myocardial viability - evaluation with thallium-201 single photon emission computed tomography (TL-201 SPECT)

    International Nuclear Information System (INIS)

    Shaikh, M.S.; Raza, M.; Kayani, A.M.; Fazal, I.

    2011-01-01

    To evaluate the effect of post-myocardial infarction Streptokinase therapy on myocardial viability, employing Thallium-201 single photon emission computed tomography (TL-201 SPECT). Design: Retrospective, experimental study. Place and duration of study: The Nuclear Cardiology Department, Armed Forces Institute of Cardiology / National Institute of Heart Diseases, Rawalpindi, from 1 April, 2009 to 31 October, 2009. Patients and Methods: Male patients, who had suffered from acute myocardial infarction (AMI), in an area supplied by the left anterior descending (LAD) artery, had infarct-related electrocardiogram (ECG) changes and received or did not receive Streptokinase therapy, were included. Those with a normal ECG, or history of revascularisation, or non-ST elevation MI, or more than one MIs, were excluded. The patients were divided into groups 1 (who received Streptokinase) and 2 (who did not receive Streptokinase). Each group contained 42 patients and all underwent scintigraphic viability study through intravenous injection of 3.0 mCi (123 MBq) of TL-201, followed by rest-redistribution SPECT imaging on a dual head, dedicated cardiac gamma camera system (Philips Cardio MD). Emory's cardiac toolbox and AutoQUANT were used for data processing and quantitative estimation of viable myocardium. Empirical scores from 0 to 2 were assigned to each of the scans, in the order of increasing viability, and these were compared across the two groups. Result: Group 1 contained 42 patients (age range = 38 to 80 years, mean = 53.98 +- 11.26 years), in whom empirical viability scoring was done. Score 0 was seen in 2 patients, score 1 was seen in 15 patients and score 2 was seen in 25 patients form this group. Group 2 also contained 42 patients (age range = 38 to 80 years, mean = 56.71 +- 9.05 years), in whom viability score of 0 was seen in 3 patients, score 1 was seen in 11 patients and score 2 was seen in 28 patients form this group. Age difference between the two groups was statistically insignificant (p = 0.223). The myocardial viability results analysed by 3 x 2 contingency table applying chi-square (X/sup 2/) test also showed no significant difference between groups 1 and 2 (p = 0.611). Conclusion: This study did not find any significant difference in myocardial viability - post-myocardial infarction - in patients who received or did not receive Streptokinase therapy. (author)

  6. Veřejno-soukromá partnerství a unijní pravidla hospodářské soutěže

    Czech Academy of Sciences Publication Activity Database

    Malíř, Jan

    2009-01-01

    Roč. 18, č. 3 (2009), s. 17-22 ISSN 1802-3843 R&D Projects: GA AV ČR IAA700680701 Institutional research plan: CEZ:AV0Z70680506 Keywords : Public Private Partnership * Economic Competition * European Union law Subject RIV: AG - Legal Sciences

  7. Pražský světící biskup? Jiří Ignác Pospíchal (1635–1699)

    Czech Academy of Sciences Publication Activity Database

    Pučalík, Marek

    2015-01-01

    Roč. 30, č. 1 (2015), s. 159-169 ISSN 0231-7494 R&D Projects: GA ČR(CZ) GA14-05167S Institutional support: RVO:67985963 Keywords : Jiří Ignác Pospíchal * the Knights of the Cross with the Red Star * Prague * Polná * bishop * archbishop Subject RIV: AB - History

  8. Preliminary Field Tests and Long-Term Monitoring as a Method of Design Risk Mitigation: A Case Study of Gdańsk Deepwater Container Terminal

    Directory of Open Access Journals (Sweden)

    Miśkiewicz Mikołaj

    2017-09-01

    Full Text Available Appropriate risk assessment plays a fundamental role in the design. . The authors propose a possible method of design risk mitigation, which follows recommendations included in Eurocode 7. The so-called “Observational Method” (OM can produce savings in costs and programmes on engineering projects without compromising safety. The case study presented is a complex design solution that deals with the heavy foundations of a gantry crane beam as one of the elements of a Deepwater Container Terminal extension. The paper presents a detailed process of the design of the rear crane beam being a part of the brand new berth, together with its static analysis, as well as the long-term results of observations, which have revealed the real performance of the marine structure. The case presented is based on excessive preliminary field tests and technical monitoring of the structure, and is an example of a successful OM implementation and design risk mitigation.

  9. Jídelní chování dětí v mateřské škole

    OpenAIRE

    Slávka Fraňková

    2017-01-01

    Development of eating behaviour is influenced by both family and social environment. Patterns of child’s eating behaviour are characterized by the approach to food, by the skill of using cutlery, by the culture of eating as well as the ability to adapt oneself to novel environment and offered meals. The analysis of children’s behaviour from the entry to a dining room till the end of a meal demonstrated developmental characteristics of eating, gender differences in the attitudes towards food a...

  10. Podnikatelský záměr - založení mateřské školky.

    OpenAIRE

    Žolnerčíková, Petra

    2013-01-01

    This Bachelor´s Thesis deals with the business plan of a first kindergarten founded in Prague with a math specialization. Its specialization will reflect the needs of technical professions. Development of children´s math skills is going to be carried out with the method of professor Hejný.

  11. České překlady Albrantova (Albrechtova) koňského lékařství

    Czech Academy of Sciences Publication Activity Database

    Černá, Alena M.

    2014-01-01

    Roč. 47, č. 3 (2014), s. 176-197 ISSN 0300-4414 R&D Projects: GA ČR GAP406/10/1140 Institutional support: RVO:68378092 Keywords : Old Czech literature * Old Czech * veterinary medicine * Master Albrant (Albrecht) Subject RIV: AI - Linguistics

  12. När E-sport blir en reseanledning : Motivation att resa som åskådare till E-sportsevenemang

    OpenAIRE

    Nordkvist, David

    2017-01-01

    E-sport is one of the fastest growing sports in the world. Hundreds ofthousands interested people travel every year as spectators to E-sport eventswhen professional E-sport stars compete against each other in the biggestgames in the world. It is an important subject to study because of the lack ofresearch and because of the recent growth of interest for E-sport in theworld. The aim of this thesis is to identify the motives behind travel to E-sport events. To answer the purpose of the thesis a...

  13. Virtuozita a performance maskulinity: Hudební etnografie jednoho pražského neromského gypsy jazzového uskupení

    OpenAIRE

    Kašparová, Žofie

    2015-01-01

    Univerzita Karlova v Praze, Filozofická fakulta Ústav hudební vědy Virtuosity and Performance of Masculinity: Music Ethnography of a Non-Roma Gypsy Jazz Group in Prague Žofie Kašparová 2014 Abstract In this ethnography I examine the gypsy jazz band Sylvanio Orchestra which is based in Prague, Czech Republic. This research consists largely of my observations at their performances, interviews with the band members as well as my personal experience of being a musician and a woman in this predomi...

  14. Filosofie jako služba. Emmanuel Lévinas v horizontu papežských reflexí

    Directory of Open Access Journals (Sweden)

    Jolana Poláková

    2017-01-01

    Full Text Available We live and think in an era in which theology increasingly lacks a generally valid philosophical rationality that could be applicable to the theological argument universally comprehensible in the context of today’s world. The paper points out the main theologically relevant features of the philosophy of Emmanuel Levinas, in which Revelation and philosophical reflection find their common way of universal rational communication.

  15. Prevalence of chronic cough in relation to upper and lower airway symptoms; the Skövde population-based study

    Directory of Open Access Journals (Sweden)

    Mats eBende

    2012-07-01

    Full Text Available The aim of this study was to determine the prevalence of chronic cough in relation to upper airway symptoms, in a cross-sectional, population-based epidemiological study. Another aim was to relate coughing to other explanatory variables and risk factors. A random sample of 1900 inhabitants from the age of 20, stratified for age and gender, was recruited. Subjects were invited for clinical examinations that included questions about general odor intolerance, respiratory symptoms, and smoking habits, and a smell identification test. In total, 1387 volunteers (73% of the sample were investigated. The overall prevalence of self-reported chronic cough was 6.3% (95% confidence interval (CI: 5.0-7.6%. Female gender, age, height, BMI, and smoking were significantly related to cough. Furthermore, nasal blockage, nasal secretion, sneezing, asthma, odor and cold air sensitivity, and aspirin intolerance also related to cough with statistical significance, indicating a close connection between chronic cough and upper airway symptoms. In keeping with other studies, this study demonstrates that chronic cough is a widespread problem in society, and is about twice as common in women than in men.

  16. Výzkum cizojazyčných tiskařských bohemik v Budapešti

    Czech Academy of Sciences Publication Activity Database

    Brabcová, Markéta

    -, č. 4 (2009), s. 20-22 ISSN 1210-8502 Institutional research plan: CEZ:AV0Z70830501 Keywords : collection of old and rare books * book culture * czech historical bibliography * national retrospective bibliography * historical bibliography Subject RIV: AF - Documentation, Librarianship, Information Studies

  17. Výzkum cizojazyčných tiskařských bohemik 16. - 18. století v Drážďanech

    Czech Academy of Sciences Publication Activity Database

    Vorlíčková, Anna

    -, č. 4 (2009), s. 19 ISSN 1210-8502 Institutional research plan: CEZ:AV0Z70830501 Keywords : collection of old and rare books * book culture * czech historical bibliography * national retrospective bibliography * historical bibliography

  18. Meziválečný katolicismus v Československu v kontextu papežských programových strategií

    Czech Academy of Sciences Publication Activity Database

    Šebek, Jaroslav

    2014-2015, č. 1 (2016), s. 241-261 ISSN 1214-8334 Institutional support: RVO:67985963 Keywords : church history * papacy * Czechoslovakia 1918-38 Subject RIV: AB - History OBOR OECD: History (history of science and technology to be 6.3, history of specific sciences to be under the respective headings)

  19. Nefinanční motivace pedagogických pracovníků v mateřské škole

    OpenAIRE

    Vodrážková, Eva

    2013-01-01

    The final thesis deals with a non-financial motivation of teachers in the kindergarten environment. It mentions some of the original motivation theories and deals with the choice of motivation for various types of teachers' personalities. The thesis also deals with the current view of the basic rules of motivation and the possibility of their use. It analyzes the capabilities of different types of non-financial motivation and their use by the school management in the people's leadership. It d...

  20. Využití metody odhadu v podniku Pražské vodovody a kanalizace, a.s.

    OpenAIRE

    Veselá, Ilona

    2010-01-01

    The paper is dedicated to the estimation method in accounting, especially to its basis, representatives and practical use. The paper is written from the theoretical definition of the generally accepted accounting principles, characterizing the accrual basis, the going concern and the prudence principle as a background of the estimation method. Furthermore, this work characterizes accruals and allowances as manifestations of the estimation method. Both expressions are first defined in general,...