Sample records for sarson

  1. Glycerolipid Profiling of Yellow Sarson Seeds Using Ultra High Performance Liquid Chromatography Coupled to Triple Time-of-Flight Mass Spectrometry

    Directory of Open Access Journals (Sweden)

    Shuning ZHENG


    Full Text Available Yellow sarson (Brassica rapa ssp. trillocularis is an important rapeseed-mustard species of Brassica rapa due to its high seed oil content. Glycerolipids and fatty acid composition affect seed germination and determine the quality of seed oil. To date, no information is available on the composition of individual glycerolipids in this species. Therefore, in this study the glycerolipid profiling of yellow sarson seeds was performed using ultra high performance liquid chromatography coupled to triple time-of-flight mass spectrometry (UPLC-Triple-TOF-MS. A fast and efficient chromatographic separation of glycerolipids was accomplished based on an UPLCTM BEH C8 column within 22 min. In ESI positive ion mode, TOF-MS scan-information dependent acquisition-product ion scan was carried out to acquire both high resolution MS and MS/MS information from one injection. According to MS/MS spectra, predominant fragmentation patterns of glycerolipids were elucidated in detail. Based on retention time, accurate mass, isotopic distribution, and fragmentation patterns, the composition of 144 glycerolipids and fatty acids were finally identified in yellow sarson seeds, including 77 triacylglycerols, 32 diacylglycerols, 18 sulfoquinovosyl-diacylglycerols, 5 monogalactosyl-diaclyglycerols, and 12 digalactosyl-diacylglycerols. Of them, the most abundant glycerolipids in yellow sarson seeds were triacylglycerols, the major storage form of seed oil in plants. In addition, diacylglycerols were found as a minor component of glycerolipids. The lowest amounts of glycerolipids detected in seeds were glycosyl-acylglycerols. The results revealed the composition and relative content of glycerolipids in yellow sarson seeds, which will provide a more comprehensive assessment of the quality of seed oil and also help to select functional cultivars with higher beneficial glycerolipids. This profiling method has the advantages of high throughput, high sensitivity and good accuracy

  2. Effect of seed-irradiation on morphological characters yield components of brassica campestris var. sarson

    International Nuclear Information System (INIS)

    Bokhari, F.S.; Ahmad, S.


    Seed of Brassica campestris (var. Sarson) were used to study the effect of radiation of different morphological and yield parameters. Survival percentage showed drastic decrease at higher doses (75 Kr and 100 Kr). Similarly all characters showed a trend of decrease with increasing dose. LD50 for Brassica was about 50 Kr. (author)

  3. New high yielding mutant varieties of mustard (Brassica campestris L. var. yellow sarson)

    International Nuclear Information System (INIS)

    Rahman, A.; Das, M.L.; Pathan, A.J.


    Mutation breeding work at the Bangladesh Institute of Nuclear Agriculture has been successful with the development of a number of promising mutants and with the release of two mutant varieties of mustard (Brassica campestris L. var. Yellow Sarson), Agrani and Safal, for commercial cultivation in Bangladesh. The mutant varieties have higher seed and oil yield with higher biomass production, tolerance to Alternaria blight and aphid under field conditions. The average seed yield of the varieties is 1726 and 1754 kg/ha as compared to 1447 kg/ha of the best check Sonali. These varieties have 42-43 per cent oil in the seed. (author). 7 refs., 3 tabs

  4. Medicinal significance of vegetables cultivated over minerals supplemented soil

    International Nuclear Information System (INIS)

    Bangash, J.A.; Arif, M.; Khan, F.; Khan, F.; Khan, A.S.


    Three winter season vegetables Fenugreek/Methi (Trigonella-foenum-graceum), Sarson (Brassica-campestris-var-sarson) and Garlic (Allium-sativum) were included in the present study to determine some of their mineral components and see if some of their mineral (Cr, Zn, Mn, Cu, Mg and Fe) content could be increased by supplementation through their roots. Thus calculated amount of Cr, Zn, Mn, Cu, Mg and Fe salts (as fertilizer) were applied in solution form to the roots of vegetables in different concentration as individual or in combinations. These vegetables were grown from seeds in the soil plot. After harvesting vegetables were dried, acid digested and analyzed for Cr, Mn, Zn, Cu, Fe and Mg on Hitachi Zeeman Japan Z-8000, Atomic Absorption Spectrophotometer. Thus in Fenugreek/Methi (Trigonella-foenum-graceum) total increase of Cr, Zn, Mn, Mg and Fe recorded was (10, 94, 10, 256 and 520) mg/Kg dry weight basis; in Sarson (Brassica-campestris-var-sarson) total increase of Cr, Zn, Mn and Mg recorded was (12, 30, 22 and 424) mg/Kg dry weight basis and ( Garlic) (Allium-sativum) total increase of Cr, Zn, Mn, Cu, Mg and Fe recorded was (14, 28, 4, 4, 116 and 10) mg/Kg dry weight basis. From the present study it can be concluded that by changing the soil minerals environment the uptake of required mineral content of vegetables, perhaps could be enhanced. This could play important role in management of diabetes control and also in the elimination of other deficiency diseases like anemia. (author)

  5. Quantitative Trait Locus Analysis of seed germination and seedling vigour in Brassica rapa reveals QTL hotspots and epistatic interactions

    NARCIS (Netherlands)

    Basnet, R.K.; Duwal, A.; Tiwari, D.N.; Xiao, D.; Monakhos, S.; Bucher, J.; Visser, R.G.F.; Groot, S.P.C.; Bonnema, A.B.; Maliepaard, C.A.


    The genetic basis of seed germination and seedling vigour is largely unknown in Brassica species. We performed a study to evaluate the genetic basis of these important traits in a B. rapa doubled haploid population from a cross of a yellow-seeded oil-type yellow sarson and a black-seeded

  6. Combining Ability Analysis and Genetic-Effects Studies for Some Important Quality Characters in Brassica napus L.

    Directory of Open Access Journals (Sweden)

    Aamar Shehzad


    Full Text Available Combining ability analysis has an important position in rapeseed breeding. To evaluate genetic and combining ability effects, three Brassica napus L. testers “Punjab Sarson, Legend and Durre-NIFA” and five lines “Duncled, K-258, ZN-R-1, ZN-R-8, ZN-M-6” were crossed using line × tester design in Randomized Complete Block Design (RCBD with three replications. Mean sum of squares of the analysis of variances (ANOVA for genotypes was highly significant for all of the traits. Most of the lines and testers exhibited significant results of mean sum of squares for combining ability. Line ‘Duncled’ was proved good general combiner for oil (8.8, protein (3.7, erucic acid (33.0, oleic acid (13.0 and glucosinolate (-19.3 over other lines and tester ‘Durree-NIFA’ for protein (6.6, erucic acid (-23.4, and linolenic acid (-5.3 over other testers. Significant specific combining ability effects were also observed. The best hybrid combinations were Legend × ZN-R-1 for oil (9.6, Punjab Sarson × Duncled for minimum erucic acid (-14.0 and linolenic acid contents (-6.0, and Legend × ZN-M-6 for maximum protein (8.2 and minimum glucosinolate contents (-11.1. The maximum oil contents were observed in ‘Legend × ZN-R-1’ (52.4%. The cross ‘Punjab Sarson × Duncled’ expressed maximum values of protein (26.5% and oleic acid (62.5% while minimum for erucic acid (2.3%, linolenic acid (5.4% and glucosinolate contents (19.3µmol/g. This research discloses the significance of non-additive genetic effects for most of the studied traits except oil contents. These studies will also help to improve nutritional values of rapeseed crop by selecting noble crosses.

  7. Varietals resistance and susceptibility in mustard (brassica campestris l.) genotypes against aphid myzus persicae (sulzer) (homoptera: aphididae)

    International Nuclear Information System (INIS)

    Sarwar, M.; Ahmad, N.; Khan, G.Z.; Tofique, M.


    The exploitation of resistant cultivars is an imperative, simple, practical and flexible way to cope with insect pests incidence. Thirty genotypes of mustard (Brassica campestris L.) were tested for their resistance and susceptibility to aphid Myzus persicae (Sulzer) exposed under natural field conditions. Data on pest tolerance of genotypes were judged by quantitative traits such as number of aphids on each infested plant and mean dry weight of seeds per genotype. Studies observed the discrepancy in overall rates of pest invasion and seed yield contained by trailed mustard genotypes. Agati sarson (P), S-9-S-97-100/45 and S-9-S-97-100/45 were the least damaged genotypes showing their moderate resistance. Amongst other genotypes, MM-I/01-5, MM-I285 and MM-I/01-6 were the most damaged showing oversensitive response. Although the majority of genotypes were found vulnerable to pest, Agati sarson (P) and S-9-S-97-100/45 due to their lowest hypersensitive response toward aphid contamination and increased pods yield could be used for the development of essential resistance in mustard plant. A marked mode of damage inflicted by aphid on the crop was noticed and the abiotic factors contributing variations in aphid infestation levels during both growing seasons were determined. Knowledge about the host plant resistance investigated can facilitate growers to choose the most appropriate cultivars as pest control strategy. (author)

  8. Harvest time residues of pendimethalin and oxyfluorfen in vegetables and soil in sugarcane-based intercropping systems. (United States)

    Kaur, Navneet; Bhullar, Makhan S


    Terminal residues of pendimethalin and oxyfluorfen applied in autumn sugarcane- and vegetables-based intercropping systems were analyzed in peas (Pisum sativum), cabbage (Brassica oleracea), garlic (Allium sativum), gobhi sarson (Brassica napus), and raya (Brassica juncea). The study was conducted in winter season in 2010-2011 and in 2011-2012 at Ludhiana, India. Pendimethalin at 0.56 kg and 0.75 kg ha(-1) was applied immediately after sowing of gobhi sarson, raya, peas, garlic, and 2 days before transplanting of cabbage seedlings. Oxyfluorfen at 0.17 kg and 0.23 kg ha(-1) was applied immediately after sowing of peas and garlic and 2 days before transplanting of cabbage seedlings intercropped in autumn sugarcane. Representative samples of these vegetables were collected at 75, 90, 100, and 165 days after application of herbicides and analyzed by high-performance liquid chromatograph (HPLC) with diode array detector for residues. The residue level of pendimethalin and oxyfluorfen in mature vegetables was found to be below the limit of quantification which is 0.05 mg kg(-1) for both the herbicides. The soil samples were collected at 0, 7, 15, 30, 45, and 60 days after the application of their herbicides. The residues of herbicides in soil samples were found to be below the detectability limit of 0.05 mg kg(-1) after 60 days in case of pendimethalin and after 45 days in case of oxyfluorfen.

  9. Genomic inferences of domestication events are corroborated by written records in Brassica rapa. (United States)

    Qi, Xinshuai; An, Hong; Ragsdale, Aaron P; Hall, Tara E; Gutenkunst, Ryan N; Chris Pires, J; Barker, Michael S


    Demographic modelling is often used with population genomic data to infer the relationships and ages among populations. However, relatively few analyses are able to validate these inferences with independent data. Here, we leverage written records that describe distinct Brassica rapa crops to corroborate demographic models of domestication. Brassica rapa crops are renowned for their outstanding morphological diversity, but the relationships and order of domestication remain unclear. We generated genomewide SNPs from 126 accessions collected globally using high-throughput transcriptome data. Analyses of more than 31,000 SNPs across the B. rapa genome revealed evidence for five distinct genetic groups and supported a European-Central Asian origin of B. rapa crops. Our results supported the traditionally recognized South Asian and East Asian B. rapa groups with evidence that pak choi, Chinese cabbage and yellow sarson are likely monophyletic groups. In contrast, the oil-type B. rapa subsp. oleifera and brown sarson were polyphyletic. We also found no evidence to support the contention that rapini is the wild type or the earliest domesticated subspecies of B. rapa. Demographic analyses suggested that B. rapa was introduced to Asia 2,400-4,100 years ago, and that Chinese cabbage originated 1,200-2,100 years ago via admixture of pak choi and European-Central Asian B. rapa. We also inferred significantly different levels of founder effect among the B. rapa subspecies. Written records from antiquity that document these crops are consistent with these inferences. The concordance between our age estimates of domestication events with historical records provides unique support for our demographic inferences. © 2017 John Wiley & Sons Ltd.

  10. A System Structure for a VHTR-SI Process Dynamic Simulation Code

    International Nuclear Information System (INIS)

    Chang, Jiwoon; Shin, Youngjoon; Kim, Jihwan; Lee, Kiyoung; Lee, Wonjae; Chang, Jonghwa; Youn, Cheung


    The VHTR-SI process dynamic simulation code embedded in a mathematical solution engine is an application software system that simulates the dynamic behavior of the VHTR-SI process. Also, the software system supports a user friendly graphical user interface (GUI) for user input/out. Structured analysis techniques were developed in the late 1970s by Yourdon, DeMarco, Gane and Sarson for applying a systematic approach to a systems analysis. It included the use of data flow diagrams and data modeling and fostered the use of an implementation-independent graphical notation for a documentation. In this paper, we present a system structure for a VHRT-SI process dynamic simulation code by using the methodologies of structured analysis

  11. Induced mutagenesis for the development of high yielding varieties in mustard

    International Nuclear Information System (INIS)

    Das, M.L.; Rahman, A.


    Variation for resistance to Alternaria brassicae (Berk.) Sacc. was induced in the oleiferous Brassica campestris cultivar yellow sarson, 'YS 52' using gamma rays. Variations were identified and isolated from the M 2 population. Screening in the subsequent generations (M 3 -M 4 ) confirmed the varying degrees of field resistance of the mutants. A total of 8 mutants of mustard developed by gamma irradiation was compared with the parental line and a released variety 'Sampad' as a check. Maximum plant height, highest number of pods and primary branches/plant were recorded in mutant '17-5-83'. The mutants '17-5-83' and '70-7-82' gave 45 and 21 per cent more grain yield respectively than the parent cultivar 'YS 52'. The mutant '17-5-83' appeared resistant while the mutants '70-7-82' and '53-11-82' were found to be moderately resistant against the disease. (author). 6 refs., 2 tables

  12. Genetic analysis of morphological traits in a new, versatile, rapid-cycling Brassica rapa recombinant inbred line population

    Directory of Open Access Journals (Sweden)

    Hedayat eBagheri


    Full Text Available A recombinant inbred line (RIL population was produced based on a wide cross between the rapid-cycling and self-compatible genotypes L58, a Caixin vegetable type, and R-o-18, a yellow sarson oil type. A linkage map based on 160 F7 lines was constructed using 100 SNP, 130 AFLP®, 27 InDel and 13 publicly available SSR markers. The map covers a total length of 1150 cM with an average resolution of 4.3 cM/marker. To demonstrate the versatility of this new population, 17 traits, related to plant architecture and seed characteristics, were subjected to QTL analysis. A total of 47 QTLs were detected, each explaining between 6 to 54% of the total phenotypic variance for the concerned trait. The genetic analysis shows that this population is a useful new tool for analyzing genetic variation for interesting traits in B. rapa, and for further exploitation of the recent availability of the B. rapa whole genome sequence for gene cloning and gene function analysis.

  13. Nutritive values of brassica campestris L. oil as affected by growth regulator treatments

    International Nuclear Information System (INIS)

    Bano, A.; Khan, N.


    The effects of plant growth regulators, viz. Indole acetic acid (IAA), Gibberellic acid (GA) and Abscisic acid (ABA) were studied on fatty acid compositions, glucosinolate content and protein content of Brassica campestris L subsp. Oleifera (common name yellow sarson). Growth regulators were applied in seed soaking solution as well as foliar spray during vegetative phase and at flowering stage. There were reductions in the amount of long chain fatty acids viz erucic acid, eicosenoic acid and increase in the amount of unsaturated fatty acid viz. linoleic acid by lAA applications. The stimulating effect of lAA which reduced amount of unsaturated fatty acid was more pronounced when applied as foliar spray at vegetative stage. But, foliar spray of ABA during flowering increased the concentration of linoleic acid and reduced the eicosenoic acid and erucic acid. The glucosinolate content was greater in seeds soaked in 10/sup -5/ M lAA than that of control but less in 10/sup -5/ M GA treated seeds than that of control. The ABA treatment (10/sup -5/M) increased the concentration of glucosinolates in the seeds IAA treatments (10/sup -5/M) increased the protein percentage in the seeds. Foliar application of GA (10/sup -5/M) during vegetative growth and ABA (10/sup -5/M) as seed soaking prior to sowing as well as foliar spry during flowering decreased the protein content of seeds. (author)

  14. Tuning growth cycles of Brassica crops via natural antisense transcripts of BrFLC. (United States)

    Li, Xiaorong; Zhang, Shaofeng; Bai, Jinjuan; He, Yuke


    Several oilseed and vegetable crops of Brassica are biennials that require a prolonged winter cold for flowering, a process called vernalization. FLOWERING LOCUS C (FLC) is a central repressor of flowering. Here, we report that the overexpression of natural antisense transcripts (NATs) of Brassica rapa FLC (BrFLC) greatly shortens plant growth cycles. In rapid-, medium- and slow-cycling crop types, there are four copies of the BrFLC genes, which show extensive variation in sequences and expression levels. In Bre, a biennial crop type that requires vernalization, five NATs derived from the BrFLC2 locus are rapidly induced under cold conditions, while all four BrFLC genes are gradually down-regulated. The transgenic Bre lines overexpressing a long NAT of BrFLC2 do not require vernalization, resulting in a gradient of shortened growth cycles. Among them, a subset of lines both flower and set seeds as early as Yellow sarson, an annual crop type in which all four BrFLC genes have non-sense mutations and are nonfunctional in flowering repression. Our results demonstrate that the growth cycles of biennial crops of Brassica can be altered by changing the expression levels of BrFLC2 NATs. Thus, BrFLC2 NATs and their transgenic lines are useful for the genetic manipulation of crop growth cycles. © 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  15. Proximate composition, minerals and vitamins content of selected vegetables grown in Peshawar

    International Nuclear Information System (INIS)

    Bangash, J.A.; Arif, M.; Khan, M.A.; Khan, F.; Amin-ur-Rrahma; Hussain, I.


    Ten vegetables namely Pot purslane, Spinach, Turnip, Garlic, Mustard (Sarson), Radish, Bitter gourd, Lady finger, Bath sponge and Brinjal were analyzed for their proximate composition, vitamin and mineral contents to evaluate their importance in human nutrition. The results showed that almost all vegetables contain appreciable amount of essential nutrients. The maximum content of moisture, carbohydrate, crude protein, crude fat, crude fiber, and ash recorded in these vegetables was (92.50 %, 26.88 %, 5.0%, 0.40%, 1.4% and 1.9%), respectively, with a minimum content (66.80 %, 3.91 %, 0.7%, 0.08%, 0.4% and 0.38%), respectively. Maximum concentration of macro minerals K, Ca, Mg, and Na, observed was (400, 210, 109, and 55) mg/100 g, respectively, with a minimum concentration (26, 15, 17, and 10) mg/100 g, respectively. Maximum concentration of micro minerals Fe, Cu, Zn, Mn and Cr observed was (29, 0.33, 3.05, 1.70, and 0.36) mg/100 g with a minimum concentration (2, 0.05, 0.43, 0.21, and 0.06) mg/100 g, respectively. Maximum concentration of water soluble vitamins i.e. thiamine, riboflavin, niacin and ascorbic acid recorded was (0.280, 0.190, 0.69, and 65) mg/100 g with a minimum concentration (0.011, 0.015, 0.23, and 4.00) mg/100 g, respectively. From this study it can be concluded that consumption of vegetables in different combinations could provide a reasonable daily recommended amount of essential nutrients for the maintenance of healthy life and normal body functioning. (author)

  16. Mind the Roots: Phenotyping Below-Ground Crop Diversity and Its Influence on Final Yield (United States)

    Nieters, C.; Guadagno, C. R.; Lemli, S.; Hosseini, A.; Ewers, B. E.


    Changes in global climate patterns and water regimes are having profound impacts on worldwide crop production. An ever-growing population paired with increasing temperatures and unpredictable periods of severe drought call for accurate modeling of future crop yield. Although novel approaches are being developed in high-throughput, above-ground image phenotyping, the below-ground plant system is still poorly phenotyped. Collection of plant root morphology and hydraulics are needed to inform mathematical models to reliably estimate yields of crops grown in sub-optimal conditions. We used Brassica rapa to inform our model as it is a globally cultivated crop with several functionally diverse cultivars. Specifically, we use 7 different accessions from oilseed (R500 and Yellow Sarson), leafy type (Pac choi and Chinese cabbage), a vegetable turnip, and two Wisconsin Fast Plants (Imb211 and Fast Plant self-compatible), which have shorter life cycles and potentially large differences in allocation to roots. Bi-weekly, we harvested above and below-ground biomass to compare the varieties in terms of carbon allocation throughout their life cycle. Using WinRhizo software, we analyzed root system length and surface area to compare and contrast root morphology among cultivars. Our results confirm that root structural characteristics are crucial to explain plant water use and carbon allocation. The root:shoot ratio reveals a significant (p physiological traits such as gas exchange, chlorophyll content, and chlorophyll a fluorescence. A thorough analysis of the root system will clarify carbon dynamics and hydraulics at the whole-plant level, improving final yield predictions.