WorldWideScience

Sample records for sardine scallop herring

  1. Is disease an important mortality factor for Pacific herring?

    Science.gov (United States)

    Hershberger, Paul

    2005-01-01

    Marine pelagic forage fishes, including sardines, anchovies, and herring, undergo large oscillations in population abundance. Although over-fishing can have a dramatic impact in reducing population size, this anthropogenic perturbation cannot fully account for all population declines because natural oscillations in marine pelagic fish biomasses occurred prior to the onset of commercial fishing. Among the herring metapopulation in Puget Sound / Straight of Georgia, the mean estimated annual mortality, exclusive of commercial fishing, increased from 20% in the late 1970’s and early 1980’s to 64-87% during 1996-1999. This natural mortality affects primarily the older age cohorts and resulted in recent decreased median ages of adult herring from age 4-6 cohorts to age 2-3 cohorts that die prior to iteroparous spawning. Surveys of wild herring from the region indicate that the protozoan parasite Ichthyophonus sp., is currently ubiquitous among Pacific herring populations in Washington and British Columbia, and prevalence of infection increase directly with herring age, from 12% among juveniles to 58% among the underrepresented age 6+ cohorts. Ichthyophonus can be highly pathogenic to immunologically naïve Pacific herring, causing 80% mortality 2 mo. after exposure in the laboratory. Current laboratory-based studies are underway to determine whether natural Ichthyophonus infections are terminal for the host, and whether these infections account for the decreasing median age of wild Pacific herring populations in recent years.

  2. Sea Scallop Survey

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The standardized NEFSC Sea Scallop Survey began in 1980 and has covered an area from Cape Hatteras to Georges Bank. The survey aims to determine the distribution and...

  3. Although South African sardine (or pilchard, Sardinops sagax) were ...

    African Journals Online (AJOL)

    spamer

    was based on assumed reliability of the hydroacoustic estimates of ... of uncertainties about the value of natural mortality) .... TAC only) also to the estimate of sardine recruitment from the ... of sardine recruitment from that survey: rev ad rev.

  4. Scallop License Limitation Program (SLLP) Permit Program

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — A federal Scallop License Limitation Program (SLLP) license is required onboard any vessel deployed in scallop fisheries in Federal waters off Alaska (except for...

  5. anchovy Engraulis capensis, sardine Sardinops sa

    African Journals Online (AJOL)

    spamer

    rates ranged between 0.05 and 0.29.h-1, with mean values of 0.09 and 0.27.h-1 for fish fed zooplankton and phytoplankton ... Linear, square root, and exponential func- tions were ...... Far Eastern sardine and their implication in the fluctuation.

  6. Scalloping at the lumbosacral canal

    Energy Technology Data Exchange (ETDEWEB)

    Reinhardt, R.

    1987-07-01

    Scalloping is an indentation of the dorsal side of the vertebral body (anterior wall of the lumbosacral or sacral canal) which typically involves several adjacent lumbal vertebral body segments and the anterior wall of the canalis sacralis. Occurrence without underlying disease is rare; it occurs most frequently with chondrodystrophy, neurofibromatosis, Morquio's syndrome, Hurler's syndrome, acromegaly, Ehlers-Danlos syndrome, Marfan's syndrome, cysts, tumors and in peridural lipomas.

  7. Scalloping at the lumbosacral canal

    International Nuclear Information System (INIS)

    Reinhardt, R.

    1987-01-01

    Scalloping is an indentation of the dorsal side of the vertebral body (anterior wall of the lumbosacral or sacral canal) which typically involves several adjacent lumbal vertebral body segments and the anterior wall of the canalis sacralis. Occurrence without underlying disease is rare; it occurs most frequently with chondrodystrophy, neurofibromatosis, Morquio's syndrome, Hurler's syndrome, acromegaly, Ehlers-Danlos syndrome, Marfan's syndrome, cysts, tumors and in peridural lipomas. (orig.) [de

  8. In upwelling regions in which anchovy Engraulis spp. and sardine ...

    African Journals Online (AJOL)

    spamer

    of anchovy and sardine is associated with these changes in abundance. Both species ... may cause sardine to shift prematurely from a phase of high to one of low .... temperature of 17°C is 51 h in the southern Benguela. Shannon: Modelling ...

  9. Ecosystem considerations of the KwaZulu-Natal sardine run ...

    African Journals Online (AJOL)

    The annual winter sardine run along the South African east coast impacts the KwaZulu-Natal (KZN) coastal system in a variety of ways. These include ecological impacts, such as enrichment of a largely oligotrophic environment, competition between migrant sardine Sardinops sagax, other migrant and resident small ...

  10. First feeding of larval herring

    DEFF Research Database (Denmark)

    Kiørboe, Thomas; Munk, Peter; Støttrup, Josianne

    1985-01-01

    The transition period from endogenous to exogenous feeding by larval herring was investigated in the laboratory for four herring stocks in order to evaluate the chances of survival at the time of fiest feeding. Observations on larval activity, feeding and growth were related to amount of yolk......, visual experience with potential prey organisms prior to first feeding and prey density. Herring larvae did not initiate exogenous feeding until around the time of yolk resorption. The timing of first feeding was not influenced by prior exposure to potential prey organisms during the yolk sac stage....... In the light of these observations, the ecological significance of the yolk sac stage is discussed. Initiation of exogenous feeding was delayed by 1-4 days at a low (7.5 nauplii .cntdot. l-1) compared to a high (120 nauplii .cntdot. l-1) prey density, but even at prey densities corresponding to the lower end...

  11. Salted herring brine as a coating or additive for herring (Clupea harengus) products — A source of natural antioxidants?

    DEFF Research Database (Denmark)

    Albertos, Irene; Gringer, Nina; Rico, Daniel

    2016-01-01

    The objective of this study was to characterise herring brine and assess its use as natural antioxidant in herring preservation. Herring brines from different marinated products (brine from fillet-ripened spice-cured herring SC, traditional barrel-salted spice-cured herring TSp and brine from...

  12. Changes in abundance of the northern Benguela sardine stock ...

    African Journals Online (AJOL)

    Changes in abundance of the northern Benguela sardine stock during the decade ... with comments on the relative importance of fishing and the environment. ... Survey-based recruitment indices suggest that the changes in the 1990s were ...

  13. 50 CFR 648.59 - Sea Scallop Access Areas.

    Science.gov (United States)

    2010-10-01

    ... each year the Elephant Turnk Access Area is open to scallop fishing as a Sea Scallop Access Area... section. LAGC IFQ vessels will be allocated 285,423 lb (129 mt) in fishing year 2010, which is 5 percent..., LAGC scallop vessels are allocated 714 trips to the Delmarva Access Area in fishing year 2010. This...

  14. Worldwide large-scale fluctuations of sardine and anchovy ...

    African Journals Online (AJOL)

    Worldwide large-scale fluctuations of sardine and anchovy populations. ... African Journal of Marine Science. Journal Home · ABOUT THIS JOURNAL · Advanced ... Fullscreen Fullscreen Off. http://dx.doi.org/10.2989/AJMS.2008.30.1.13.463.

  15. Worldwide large-scale fluctuations of sardine and anchovy ...

    African Journals Online (AJOL)

    Worldwide large-scale fluctuations of sardine and anchovy populations. ... African Journal of Marine Science. Journal Home · ABOUT THIS JOURNAL · Advanced ... http://dx.doi.org/10.2989/AJMS.2008.30.1.13.463 · AJOL African Journals ...

  16. Worldwide, where anchovies Engraulis spp. and sardine Sardinops ...

    African Journals Online (AJOL)

    spamer

    sardine Sardinops sagax co-occur, the two species alternate in relative ... of extinction in the wild within 100 years (Croxall et al. 1996). This paper ... Abundance of this fish decreased in the 1960s and early 1970s and it was replaced by ...

  17. SaRDIn - A Safe Reconfigurable Distributed Interlocking

    DEFF Research Database (Denmark)

    Fantechi, Alessandro; Gnesi, S.; Haxthausen, Anne Elisabeth

    2016-01-01

    Current computer-based interlocking systems most often have a centralized design, with all logic residing in a single computer. Centralized interlockings are complex to design. Following the general trend in Cyber-Physical Systems, the SaRDIn (Safe Reconfigurable Distributed Interlockings) concept...

  18. Annual ring validation of the south african sardine Sardinops sagax ...

    African Journals Online (AJOL)

    A total of 21 sardine Sardinops sagax otoliths, collected in winter and spring 1994, were examined with a scanning electron microscope to validate growth zones using daily growth increments. Increment numbers and widths for the first, second and third annual growth zones served to back-calculate hatching dates and to ...

  19. The southern African sardine Sardinops sagax (also known as ...

    African Journals Online (AJOL)

    denise

    CHANGES IN ABUNDANCE OF THE NORTHERN BENGUELA SARDINE STOCK. DURING THE ... date of young fish, using standard growth rates, indi- ... and catch per unit effort (cpue) is recognized to be a ... is developed, age-based assessment models cannot be ... The methods are described in various cruise reports.

  20. Essential habitat for sardine juveniles in Iberian waters

    Directory of Open Access Journals (Sweden)

    Sílvia Rodríguez-Climent

    2017-09-01

    Full Text Available In a period when the Iberian sardine stock abundance is at its historical minimum, knowledge of the sardine juvenile’s distribution is crucial for the development of fishery management strategies. Generalized additive models were used to relate juvenile sardine presence with geographical variables and spawning grounds (egg abundance and to model juvenile abundance with the concurrent environmental conditions. Three core areas of juvenile distribution were identified: the Northern Portuguese shelf (centred off Aveiro, the coastal region in the vicinity of the Tagus estuary, and the eastern Gulf of Cadiz. Spatial differences in the relationship between juvenile presence and egg abundances suggest that essential juvenile habitat might partially differ from the prevailing spawning grounds. Models also depicted significant relationships between juvenile abundance, temperature and geographical variables in combination with salinity in the west and with zooplankton in the south. Results indicate that the sardine juvenile distribution along the Iberian Peninsula waters are an outcome of a combination of dynamic processes occurring early in life, such as egg and larva retention, reduced mortality and favourable feeding grounds for both larvae and juveniles.

  1. Development of a compressed product made with sardine.

    Science.gov (United States)

    Bourges, H; Morales de León, J C; Sierra, H

    1985-09-01

    The per capita consumption of marine products is very low in Mexico, averaging less than 4 g/day. This fact has been partially attributed to the costly techniques used in their preservation, which result in high market prices unaffordable for large segments of the population. Previous research lead to the development of pressed and salted patties based on lean fish species, the low cost and easy preservation of which would contribute to a higher fish consumption among the low socio-economic strata of the Mexican population. The present work was directed to adapt the procedure to sardine, which is more abundant and less expensive than lean fish species. Since defatting the sardine lead to poor sensorial characteristics of the patties, measures were taken to protect the fat from oxidation, through the use of BHT and citric acid. The best results were obtained with descaled sardine, and with the addition of 8% NaCl, 10% corn flour and a condiment mixture. The resulting product had 32% of high-quality protein and a shelf life of at least six months under environmental conditions. Its cost per gram of protein was one-third lower than the price of fresh or canned sardine. Sensorial tests revealed an acceptability of 82%.

  2. Revised estimates of abundance of South African sardine and ...

    African Journals Online (AJOL)

    Hydro-acoustic surveys have been used to provide annual estimates of May recruitment and November spawner biomass of the South African sardine Sardinops sagax and anchovy Engraulis encrasicolus resources since 1984. These time-series of abundance estimates form the backbone of the assessment of these ...

  3. Unraveling biocomplexity of Northeast Atlantic herring stocks using SNP markers

    DEFF Research Database (Denmark)

    Bekkevold, Dorte; Limborg, Morten; Helyar, Sarah

    2012-01-01

    Atlantic herring (Clupea harengus) exhibit biocomplexity, with widespread, geographically explicit populations that perform long‐range migration to common feeding and wintering areas, where they are exploited by fisheries. This means that exploited stocks do not describe discrete units, thereby c...... and spatial dynamics applicable to stock assessment methods, as well as presenting a traceability tool for certification of herring and herring products...

  4. QUALITY CHANGES OF SARDINES (Sardinella neopilchardus) AT INDONESIAN AMBIENT TEMPERATURE

    OpenAIRE

    Farida Ariyani

    2018-01-01

    In tropical countries such as Indonesia, high ambient temperature and a lack of icing cause very rapid spoilage of fish. Leaving fish uniced while waiting for processing is a common practice resulting in lower quality of the end products, and consequently lower price of the products. To study the spoilage pattern of sardines (Sardinella neophilchardus) in a tropical region, and to investigate a simple and quick method to judge the degree of spoilage, observations on the deterioration of sardi...

  5. Microplastic and mesoplastic contamination in canned sardines and sprats.

    Science.gov (United States)

    Karami, Ali; Golieskardi, Abolfazl; Choo, Cheng Keong; Larat, Vincent; Karbalaei, Samaneh; Salamatinia, Babak

    2018-01-15

    No report was found on the occurrence of microplastics in processed seafood products that are manufactured for direct human consumption. This study investigates the potential presence of micro- and mesoplastics in 20 brands of canned sardines and sprats originating from 13 countries over 4 continents followed by their chemical composition determination using micro-Raman spectroscopy. The particles were further inspected for their inorganic composition through energy-dispersive X-ray spectroscopy (EDX). Plastic particles were absent in 16 brands while between 1 and 3 plastic particles per brand were found in the other 4 brands. The most abundant plastic polymers were polypropylene (PP) and polyethylene terephthalate (PET). The presence of micro- and mesoplastics in the canned sardines and sprats might be due to the translocation of these particles into the edible tissues, improper gutting, or the result of contamination from the canneries. The low prevalence of micro- and mesoplastics sized >149μm, and the absence of potentially hazardous inorganic elements on them, might indicate the limited health risks associated with their presence in canned sardines and sprats. Due to the possible increase in micro- and mesoplastic loads in seafood products over time, the findings of this study suggest their quantification to be included as one of the components of food safety management systems. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Extraction of high added value biological compounds from sardine, sardine-type fish and mackerel canning residues--a review.

    Science.gov (United States)

    Ferraro, Vincenza; Carvalho, Ana P; Piccirillo, Clara; Santos, Manuela M; Castro, Paula M L; Pintado, Manuela E

    2013-08-01

    Different valuable compounds, which can be employed in medicine or in other industries (i.e. food, agrochemical, pharmaceutical) can be recovered from by-products and waste from the fish canning industries. They include lipids, proteins, bio-polymers, minerals, amino acids and enzymes; they can be extracted from wastewaters and/or from solid residues (head, viscera, skin, tails and flesh) generated along the canning process, through the filleting, cooking, salting or smoking stages. In this review, the opportunities for the extraction and the valorisation of bioactive compounds from sardine, sardine-type fish and mackerel canning residues are examined and discussed. These are amongst the most consumed fishes in the Mediterranean area; moreover, canning is one of the most important and common methods of preservation. The large quantities of by-products generated have great potentials for the extraction of biologically desirable high added value compounds. Copyright © 2013 Elsevier B.V. All rights reserved.

  7. Synthetic biology as red herring.

    Science.gov (United States)

    Preston, Beth

    2013-12-01

    It has become commonplace to say that with the advent of technologies like synthetic biology the line between artifacts and living organisms, policed by metaphysicians since antiquity, is beginning to blur. But that line began to blur 10,000 years ago when plants and animals were first domesticated; and has been thoroughly blurred at least since agriculture became the dominant human subsistence pattern many millennia ago. Synthetic biology is ultimately only a late and unexceptional offshoot of this prehistoric development. From this perspective, then, synthetic biology is a red herring, distracting us from more thorough philosophical consideration of the most truly revolutionary human practice-agriculture. In the first section of this paper I will make this case with regard to ontology, arguing that synthetic biology crosses no ontological lines that were not crossed already in the Neolithic. In the second section I will construct a parallel case with regard to cognition, arguing that synthetic biology as biological engineering represents no cognitive advance over what was required for domestication and the new agricultural subsistence pattern it grounds. In the final section I will make the case with regard to human existence, arguing that synthetic biology, even if wildly successful, is not in a position to cause significant existential change in what it is to be human over and above the massive existential change caused by the transition to agriculture. I conclude that a longer historical perspective casts new light on some important issues in philosophy of technology and environmental philosophy. Copyright © 2013 Elsevier Ltd. All rights reserved.

  8. The role of 'conservatism' in herring migrations

    NARCIS (Netherlands)

    Corten, A.

    2002-01-01

    Herring (Clupea harengus) migrations tend to remain constant over periods of several years or even decades, despite environmental variation. When a migration pattern is changed, apparently in response to an environmental stimulus, the change in migratory behavior sometimes lasts longer than the

  9. Socio-economic implications of the KwaZulu-Natal sardine run for ...

    African Journals Online (AJOL)

    The economic and social effects of the annual sardine run on the indigenous community on the south coast of KwaZulu-Natal, South Africa, were assessed using data gathered from questionnaires and personal interviews with 329 members of the community. Their knowledge, perceptions and attitudes about the sardine run ...

  10. Optimization of a chemical method for skinning of sardines (Sardina pilchardus during canning processing

    Directory of Open Access Journals (Sweden)

    Manuela Vaz Velho

    2014-06-01

    Full Text Available Most of sardine (Sardina pilchardus catches is used for canning purposes. The most common product presentation is a beheaded sardine with skin and bones packed in a tin can. Canned sardines can also be presented skinless and boneless. For this last type of product, after beheading and evisceration, sardines are placed in trays, cooked and then skinned by hand, one by one, and placed in the tins, a process involving high labour costs. The aim of this work was to develop a chemical process for peeling raw sardines and its subsequent application in a canning industry processing line just after the beheading and evisceration step and before cooking. Potassium hydroxide treatments (pellets a.r. 85% KOH were applied at concentrations of 2, 3 and 4% (v/v, distilled water. Frozen sardines were beheaded and eviscerated after thawing and immersed in the different potassium hydroxide solutions at 93ºC (pH respectively 13, 13 and 13.02 for 3 min and further washed with distilled water at 100°C. In this first set of experiments, fat sardines were used (average of 9.86% of fat, w/w. The best performance, with respect to skin removal, was achieved with the 2% potassium hydroxide immersion (pH 13. With this treatment the skin was totally removed after immersion. With the other tested concentrations portions of skin were always visible and in some cases changes in texture with breakdown of muscle structure and changes of colour occurred. It was decided to perform a second set of experiments using the 2% KOH treatment, but this time applied to low fat sardines (average of 4.77% of fat, w/w, following the same subsequent procedures. The results showed that the lower fat sardines are more prone to surface changes of colour and major muscle breaks than fat sardines after the potassium hydroxide treatment. In the canning industry for this type of product (skinless and boneless only fat sardines are used to assure the total removal of skin. This treatment of 2% KOH

  11. QUALITY CHANGES OF SARDINES (Sardinella neopilchardus AT INDONESIAN AMBIENT TEMPERATURE

    Directory of Open Access Journals (Sweden)

    Farida Ariyani

    2018-01-01

    Full Text Available In tropical countries such as Indonesia, high ambient temperature and a lack of icing cause very rapid spoilage of fish. Leaving fish uniced while waiting for processing is a common practice resulting in lower quality of the end products, and consequently lower price of the products. To study the spoilage pattern of sardines (Sardinella neophilchardus in a tropical region, and to investigate a simple and quick method to judge the degree of spoilage, observations on the deterioration of sardines were carried out by incubating fish in a humidity chamber adjusted to 28-30oC. RH 70-80% for up to 14 h. The changes of sensory attributes based on demerit point score. pH, and total volatile base nitrogen (TVB-N were monitored to determine the degree of spoilage. Although the appearance of sardines was still bright after 6-h incubation time, fish were not as fresh as those without incubation and the belly and tissue were softened. After incubation time of 10 h, fish showed slight to moderate signs of spoilage with demerit point score of 25 out of the maximum score of 39, and a TVB-N content of 20 mg%N. This is similar to the fish quality commonly used in the commercial production of dried-salted fish in Indonesia. Fish incubated for 12 h or more appeared completely spoiled and were rejected by sensory panel. A high correlation between demerit point score and TVB-N was noted, therefore the demerit point system which was more suitable, quicker, and easier could be used to determine the degree of fish spoilage.

  12. "Everything revolves around the herring": the Heiltsuk-herring relationship through time

    Directory of Open Access Journals (Sweden)

    Alisha M. Gauvreau

    2017-06-01

    Full Text Available Pacific herring (Clupea pallasii is foundational to many social-ecological systems of the North American coast. The indigenous people of Heiltsuk First Nation on the central coast of British Columbia, Canada have depended on this forage fish for food, social, ceremonial, and economic purposes for millennia. Our research documents social, ecological, and cultural aspects of Heiltsuk First Nation's relationship with Pacific herring and how this relationship has changed over time. We describe and discuss (1 how Heiltsuk social institutions, local and traditional ecological knowledge, and worldview have informed herring management strategies from pre-contact times until present, and (2 how post-contact changes in state-led herring management and other social and institutional developments in British Columbia have affected the role and transmission of Heiltsuk local knowledge and management of herring. By working in close partnership with Heiltsuk decision-makers, and by conducting interviews with Heiltsuk knowledge holders, we ensured that the data gathered would be relevant, applicable, and valuable to the Heiltsuk community. Our research therefore serves as an example of how state fisheries agencies could improve relationships with indigenous communities by engaging in more collaborative data collection, and our results suggest the potential for joint learning and improvement in fisheries management through collaboration during the design of management and harvesting plans. Our research has relevance at the global level because we identify some of the steps that may be taken to help overcome institutionalized inertia and attain more equitable power relationships for sustainable fisheries management.

  13. A Coordinate-Based Proof of the Scallop Theorem

    OpenAIRE

    Ishimoto, Kenta; Yamada, Michio

    2012-01-01

    We reconsider fluid dynamics for a self-propulsive swimmer in Stokes flow. With an exact definition of deformation of a swimmer, a coordinate-based proof is first given to Purcell's scallop theorem including the body rotation.

  14. Diet of scalloped hammerhead shark in eastern Gulf of Mexico

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Juvenile scalloped hammerhead sharks, Sphyrna lewini, were collected in northwest Florida to examine foraging ecology, bioenergetics, and trophic level (30-60 cm FL...

  15. Ichthyophonus hoferi disease in the herring in Norwegian waters

    OpenAIRE

    Hjeltnes, Brit K.; Skagen, Dankert W.

    1992-01-01

    After the discovery of I. hoferi disease in Norwegian spring spawning herring in summer 1991, a program for systematic surveillance of the disease in the herring in Norwegian waters was developed. The programme, which aims primarily at monitoring the prevalence of the disease, is described. Macroscopic lesions in the heart emerged as the standard diagnostic criterium. The results so far indicate that the situation for Norwegian spring spawning herring is not alarming, ...

  16. Sardine Fish Oil By Sentrifugation and Adsorbent for Emulsion

    Directory of Open Access Journals (Sweden)

    Kristina Haryati

    2017-04-01

    Full Text Available Sardine fish meal by-product contain eicosapentaenoic acid (EPA and docosahexaenoic (DHA and it can be made as emulsion. The purpose of this study were to determine the best fish oil emulsion by mixingthe oil phase (lecithin 3% and oil and water phase (carboxymethyl cellulose/CMC 2% and fruit juice and then stored until creaming, and the emulsion is analyzed their viscosity, pH, percent of stability and longseparation. Sardine oil is separated from the emulsion and tested oxidation parameters. The best emulsion was fish oil emulsion after refined without citric acid (RTS with viscosity (2470.31 cP, pH (5.64, percent of stability (56.14% and long separation (14 days. Primary and secondary oxidation parameters of RTS  were FFA (14.87%, PV (14.43 meq/kg, AV (32.57 meq KOH/g, AnV (17.3 meq/kg, and Totox (46.16 meq/kg.

  17. A real-time PCR approach to detect predation on anchovy and sardine early life stages

    Science.gov (United States)

    Cuende, Elsa; Mendibil, Iñaki; Bachiller, Eneko; Álvarez, Paula; Cotano, Unai; Rodriguez-Ezpeleta, Naiara

    2017-12-01

    Recruitment of sardine (Sardina pilchardus Walbaum, 1792) and anchovy (Engraulis encrasicolus Linnaeus, 1758) is thought to be regulated by predation of their eggs and larvae. Predators of sardine and anchovy can be identified by visual taxonomic identification of stomach contents, but this method is time consuming, tedious and may underestimate predation, especially in small predators such as fish larvae. Alternatively, genetic tools may offer a more cost-effective and accurate alternative. Here, we have developed a multiplex real-time polymerase chain reaction (RT-PCR) assay based on TaqMan probes to simultaneously detect sardine and anchovy remains in gut contents of potential predators. The assay combines previously described and newly generated species-specific primers and probes for anchovy and sardine detection respectively, and allows the detection of 0,001 ng of target DNA (which corresponds to about one hundredth of the total DNA present in a single egg). We applied the method to candidate anchovy and sardine egg predators in the Bay of Biscay, Atlantic Mackerel (Scomber scombrus) larvae. Egg predation observed was limited primarily to those stations where sardine and/or anchovy eggs were present. Our developed assay offers a suitable tool to understand the effects of predation on the survival of anchovy and sardine early life stages.

  18. Reconstruction of trophic pathways between plankton and the North Iberian sardine (Sardina pilchardus using stable isotopes

    Directory of Open Access Journals (Sweden)

    Antonio Bode

    2004-03-01

    Full Text Available Feeding on phyto- and zooplankton by juvenile (< 1 year old and adult sardines (Sardina pilchardus was inferred from analyses of natural abundance of stable carbon and nitrogen isotopes in samples from the northwestern Iberian Peninsula (Spain collected at the beginning of the upwelling season and peak spawning period of sardine. Plankton samples were fractionated through nets of 20, 200, 500, 1000 and 2000 ?m mesh-size and the muscle protein of individual sardines was isolated before isotopic determinations. Up to six planktonic components and two sardine feeding types were identified from the modes in the frequency distributions of isotope abundance values. Also, the most probable pathways for carbon and nitrogen flows between compartments were analysed. The resulting food web revealed a relatively large degree of omnivory, both in plankton and sardine components, which confirms that complex trophic interactions could also occur in pelagic upwelling ecosystems. Young sardines had isotope abundance values clustered around a single mode in the frequency distribution, while adult sardines displayed two main modes. These modes are interpreted as representative of two extreme feeding types: one related to the individual capture of zooplankton prey and the other to unselective filter-feeding. Although both types of feeding could include micro- (20-200 ?m and mesozooplankton (200-2000 ?m prey, phytoplankton appears to be ingested mainly by filter-feeding. However, even adult sardines must be mainly zoophagous to achieve the observed isotopic abundance values, taking into account current assumptions on stable isotope enrichment through trophic levels. From the differences in the resulting pathways using either carbon or nitrogen isotopes, we interpreted that sardines acquire most of the protein nitrogen from zooplankton while a substantial fraction of their carbon would derive from phytoplankton. These interpretations agree with the information

  19. HISTAMINE IN CANNED SARDINES HISTAMINA EM CONSERVAS DE SARDINHA

    Directory of Open Access Journals (Sweden)

    Fancislene Bernardes Tebalti do Carmo

    2010-04-01

    Full Text Available In this study, the presence of histamine in 122 samples of canned sardines produced with three different species by three industries located in the municipalities of Sao Goncalo and Niteroi was evaluated. The samples were divided into five lots with copies of sardines from Venezuela (Sardinella aurita, Morocco (S. pilchardus and Brazil (S. brasiliensis. The initial quality of raw material was evaluated by sensorial parameters and by the histamine level using a semi-quantitative method of thin-layer chromatography. The results of the samples from Venezuela and Morocco showed values below 5 mg/100g, and the national samples showed values similar or greater than 10 mg/100g. It follows that there is need for greater control and monitoring of temperature from capture to processing, to guarantee good quality to the final product, and to avoid risk of poisoning to the consumer.

    KEY WORDS: Canned fish, histamine, quality, sardines.

    O presente estudo avaliou a presença de histamina em 122 amostras de sardinha em conserva, produzidas com três diferentes espécies, por três indústrias, localizadas nos municípios de São Gonçalo e Niterói. As amostras foram divididas em cinco lotes com exemplares de sardinhas provenientes da Venezuela (Sardinella aurita, Marrocos (S. pilchardus e do Brasil (S. brasiliensis. Avaliou-se a qualidade inicial da matéria-prima por meio de parâmetros sensoriais e pelo teor de histamina utilizando-se o método de cromatografia em camada delgada. As amostras oriundas da Venezuela e Marrocos apresentaram valores abaixo de 5 mg/100 g e as nacionais, valores semelhantes ou superiores a 10 mg/100g. Conclui-se que há necessidade de um maior controle e monitorização da temperatura da sardinha desde a captura até o processamento, para que o produto final apresente boa qualidade e não represente perigo de intoxicação ao consumidor.

    PALAVRAS-CHAVES: Conserva, histamina, qualidade, sardinha.

  20. Frozen herring as raw material for spice-salting

    NARCIS (Netherlands)

    Stefansson, G.; Nielsen, H.H.; Skara, T.; Schubring, R.; Oehlenschlager, J.; Luten, J.

    2000-01-01

    One batch of herring (Clupea harengus) was spice-salted fresh and as thawed after 32 days of frozen storage at -24 °C. After salting, samples of both groups were sent to participating laboratories in Iceland, Denmark, Norway, Germany and England. The herring was kept at 5 ± 1 °C and sampled three

  1. Environmental Factors and Natural Resource Stock: Atlantic Herring case

    Energy Technology Data Exchange (ETDEWEB)

    Cho, J.H. [Korea Maritime Institute, Seoul (Korea); John, M. Gate [University of Rhode Island, Kingston (United States)

    2001-12-01

    Atlantic herrings have held the important position as fish-baits in the marine ecosystem such as major baits in fishing lobsters. The Atlantic herring is sensitively influenced by the environmental factors of the marine ecosystem, such as the temperature of seawater, the amount of planktons, and the submarine deposit of the habitat. In the immature phase of herrings, especially, they are very sensitive of the low temperature of seawater. This study analyzes the correlation between two-year-old imported herring resources and the temperature of seawater, measured by a satellite. The area of measuring temperature is limited to the spawning ground of Atlantic herrings. As results of the analysis, the coefficient is 0.69, which means that the environmental factors should be very seriously considered in explaining the change of fishing resources. 12 refs., 4 figs., 1 tab.

  2. Oil sardine and Indian mackerel: Their fishery, problems and coastal oceanography

    Digital Repository Service at National Institute of Oceanography (India)

    Madhupratap, M.; Shetye, S.R.; Nair, K.N.V.; Nair, S.R.S.

    The sardine and mackerel fishery is commercially exploited successfully along the west coast of India. The fish itself times its appearance to exploit the productive southwest monsoon period. The chain of events-physical, chemical and biological...

  3. The Omani Coastal Traditional Sardine Fishery 1994-2007: A Review

    Directory of Open Access Journals (Sweden)

    Saud Al-Jufaili

    2011-01-01

    Full Text Available The Omani coastal fishery is categorized into small pelagics, large pelagics, demersals, shellfishes, and sharks and rays. During the period 1994-2007 the small pelagics contributed 39 and 21% to the total Omani fisheries landings and values respectively. During 1994-2007 sardines alone contributed 76 and 51% to the total small pelagics landings and values, respectively. Al-Batina and Muscat regions contributed the highest to the total landings; among other Omani regions, Musandam, Muscat, and Sharqiya and Al-Wusta regions. Total sardines prices ranges from 114 to 225 Omani Rials per ton depending on where the sardines are coming from. On the other hand, Al-Batina and Muscat regions contributed the most in the total sardine values. Few gonadosomatic index studies indicated two major spawning peaks: a major one occurred during March-April and a minor one in September-October.

  4. Remotely sensed chlorophyll: A putative trophic link for explaining variability in Indian oil sardine stocks

    Digital Repository Service at National Institute of Oceanography (India)

    George, G.; Meenakumari, B.; Raman, M.; Kumar, S.; Vethamony, P.; Babu, M.T.; Verlecar, X.

    and explain the phenomena of interannual variability. Earlier research has indicated that the probable appearance and disappearance of sardines is an active movement in search of food and favourable conditions. But no specific study has been carried out...

  5. 77 FR 73957 - Fisheries of the Northeastern United States; Atlantic Sea Scallop Fishery; Closure of the...

    Science.gov (United States)

    2012-12-12

    ... than 75 mm (3 in) shell height in the ETA was 994, compared to 24 in 2011. Most of the scallop biomass... fishing effort in this area, which could reduce long-term scallop biomass and optimum yield from the ETA... them to grow. Following closure of the ETA, scallop biomass increased steadily in the area. When the...

  6. Improving smoked herring quality by gamma irradiation

    International Nuclear Information System (INIS)

    Zahran, D.A.; Abd El-Wahab, S.A.; Hendy, B.A.

    2009-01-01

    Smoked herring which is a highly purchasable product in Egypt, was exposed to different gamma irradiation doses (1.5,3.0 and 5.0 kGy) and stored at environmental temperature (12± 2 deg C) until spoilage of the control. Microbiological, chemical and sensory analyses were performed throughout storage to monitor the quality attributes. It is worthy to mention that irradiation reduced the population of bacteria and the effect was more pronounced at the highest dose used (5.0 kGy). At the same time 1.5 kGy completely eliminated staphylococcus aureus (coagulase + ve) and coliforms. By chemical analysis, there was significant decrease in average moisture content by different gamma irradiation doses and storage. Although the average thiobarbituric acid reactive substances (TBARS) increased slightly by γ-irradiation, this increase was highly significant by storage . At the same time there was a significant (p< 0.05)decrease in the average trimethylamine (TMA) value of all irradiated samples compared with unirradiated control, this value increased significantly by storage. interestingly, the average histamine value decreased significantly in all irradiated samples. The sensory analysis revealed a highly significant difference in the average acceptability scores between different irradiation doses used and also by storage. Therefore it could be concluded that the quality of smoked herring during storage at environmental temperature (12 ± 2 deg C) could be improved by using 5.0 kGy γ -irradiation

  7. Are life histories of Norwegian fjord herring populations of Pacific ancestry similar to those of Atlantic or Pacific herring?

    Science.gov (United States)

    Mikkelsen, Nina; Pedersen, Torstein; dos Santos Schmidt, Thassya Christina; Falk-Petersen, Inger-Britt; Slotte, Aril

    2018-04-01

    Herring from two unexploited fjord populations, Lake Rossfjord (LRH, n = 100) and Balsfjord (BFH, n = 420) in northern Norway, were sampled in 2014 and 2015. Life history characteristics were analysed and compared to the oceanic Norwegian spring-spawning herring (NSSH), and other Atlantic and Pacific herring stocks. Genetic studies have shown that LRH and BFH are ancestors evolved from Pacific herring that hybridized with Atlantic herring. This study shows that both LRH and BFH mature at a relatively early age, at 2-3 years and ca. 4 years respectively, compared to ca. 5 years for NSSH. The spawning stocks of LRH and BFH consist of small fish and contain relatively few age classes. Both fjord populations have slow growth after sexual maturity; LRH has a very low asymptotic length (L∞ = 19.8 cm), while that of BFH is higher (L∞ of 28.5 cm); both these values being lower than that of NSSH (L∞ of ca. 37 cm). The somatic relative fecundity of LRH is 176.6 oocytes g- 1, while the somatic relative fecundity of recruit and repeat BFH spawners is 152.4 and 183.1 oocytes g- 1, respectively. These estimates are lower than those for NSSH and other Atlantic herring fjord populations, but comparable with other Pacific herring. Due to the smaller body sizes of the spawners in the LRH and BFH populations, absolute fecundity is much lower than in NSSH. The gonadosomatic indices of prespawning fish are similar in LRH and BFH, being slightly higher compared to the NSSH, but lower than values reported for Pacific herring. The natural mortality rates of LRH and BFH (M = 0.64 year- 1 and M = 0.76 year- 1, respectively) are much higher than in NSSH (M = 0.15 year- 1) and most other Atlantic herring populations, except the Lusterfjord herring. However, these high mortality rates are similar to those of several Pacific herring populations. It is concluded that LRH and BFH show low somatic growth and high natural mortality rate. These life history characteristics differ from those of

  8. Combination of Sardine and Shark Oil High Content of Omega-3 and Squalene

    Directory of Open Access Journals (Sweden)

    Muhamad Musbah

    2017-05-01

    Full Text Available Sardine oil contain high concentration of  EPA but low of  DHA while shark is reverse. Shark oil  high contain of DHA and squalene but low EPA. This research aim to fortify the quality of  fish oil withomega-3 and squalen and improve the quality of fish oil. The combination of fish oil (sardine:shark 1: 1, 1: 2, 1: 3, 1: 4, 2: 1, 3: 1 and 4:1 showed significant results on peroxide, anisidine, and total oxidation value, however free fatty acids analysis did not show the influence to the content value.  The best oxidation parameters value werefound (sardine: shark (1:4 with peroxide was 5.44±0.06 mEq/kg, anisidine was 8.3±0.72 mEq/kg and total oxidation was 19.27±0.7mEq/kg. Fatty acids profile between  sardines and shark oil containedvarious SFA, MUFA and PUFA. Sardine oil which was added more to combination ratio will increase omega-3. Sample 1:4 had 43.16% squalene.

  9. Sensory Properties of Frozen Herring (Clupea harengus) from Different Catch Seasons and Locations

    DEFF Research Database (Denmark)

    Hyldig, Grethe; Jørgensen, Bo Munk; Undeland, Ingrid

    2012-01-01

    Freezing of herring (Clupea harengus) for human consumption is increasing in the Nordic herring industry, either onboard the fishing vessels or right after landing. The quality of frozen herring as a raw material does not only depend on the frozen storage conditions applied, but also...... on compositional features, something which in turn can vary with season and catching ground. To unravel the link between biological variations, basic muscle composition, and sensory properties of frozen herring, a unique herring raw material was caught by commercial fishing vessels at three locations: around...... the utilization of herring for frozen storage for human consumption....

  10. Population diversity in Pacific herring of the Puget Sound, USA.

    Science.gov (United States)

    Siple, Margaret C; Francis, Tessa B

    2016-01-01

    Demographic, functional, or habitat diversity can confer stability on populations via portfolio effects (PEs) that integrate across multiple ecological responses and buffer against environmental impacts. The prevalence of these PEs in aquatic organisms is as yet unknown, and can be difficult to quantify; however, understanding mechanisms that stabilize populations in the face of environmental change is a key concern in ecology. Here, we examine PEs in Pacific herring (Clupea pallasii) in Puget Sound (USA) using a 40-year time series of biomass data for 19 distinct spawning population units collected using two survey types. Multivariate auto-regressive state-space models show independent dynamics among spawning subpopulations, suggesting that variation in herring production is partially driven by local effects at spawning grounds or during the earliest life history stages. This independence at the subpopulation level confers a stabilizing effect on the overall Puget Sound spawning stock, with herring being as much as three times more stable in the face of environmental perturbation than a single population unit of the same size. Herring populations within Puget Sound are highly asynchronous but share a common negative growth rate and may be influenced by the Pacific Decadal Oscillation. The biocomplexity in the herring stock shown here demonstrates that preserving spatial and demographic diversity can increase the stability of this herring population and its availability as a resource for consumers.

  11. Assessment of the sardine (Sardina pilchardus Walbaum, 1792 fishery in the eastern Mediterranean basin (North Aegean Sea

    Directory of Open Access Journals (Sweden)

    K. ANTONAKAKIS

    2011-06-01

    Full Text Available The aim of this study was to describe the biometric characteristics of the European sardine (Sardina pilchardus catches and assess the current status of sardine stock in North Aegean Sea based on population characteristics and abundance trends. The stock was dominated by age groups 1 and 2, not exceeding age group 4. The sardine stock in this area was assessed through an Integrated Catch-at-Age model which implements a separable Virtual Population Analysis on catch at age data with weighted tuning indices. Sardine landings data derived from the commercial purse seine fishery over the period 2000-2008 were combined with the age structure of the stock as resulted from fisheries independent acoustic surveys. Sensitivity analysis of the impact of natural mortality values on stock assessment results was applied. Additionally forecast of the sardine population parameters and catches under different exploitation scenarios was implemented in a medium term basis. Results indicated that the North Aegean Sea sardine stock is considered fully exploited with the fishery operating close but over the empirical exploitation level for sustainability. Finally, the status of the sardine stock in N. Aegean Sea is discussed in relation to the sardine stocks from the western and the central Mediterranean basin.

  12. 76 FR 79610 - Fisheries of the Northeastern United States; Atlantic Herring Fishery; Adjustment to 2012 Annual...

    Science.gov (United States)

    2011-12-22

    ... account for catch overages in 2010 and to prevent overfishing. DATES: Public comments must be received no... FR 48874). Although herring is not overfished and is not experiencing overfishing, the herring annual...

  13. NEFSC 1998 Atlantic Herring Acoustic/Midwater Trawl Survey (DE9810, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  14. NEFSC 2011 Atlantic Herring Acoustic/Midwater Trawl Survey (DE1108, EK60)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  15. NEFSC 2002 Atlantic Herring Acoustic/Midwater Trawl Survey (DE0208, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  16. NEFSC 2006 Atlantic Herring Acoustic/Midwater Trawl Survey (DE0615, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  17. NEFSC 1999 Atlantic Herring Acoustic/Midwater Trawl Survey (DE9909, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  18. NEFSC 2008 Atlantic Herring Acoustic/Midwater Trawl Survey (DE0809, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  19. NEFSC 2003 Atlantic Herring Acoustic/Midwater Trawl Survey (DE0308, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  20. NEFSC 2004 Atlantic Herring Acoustic/Midwater Trawl Survey (DE0413, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  1. NEFSC 2010 Atlantic Herring Acoustic/Midwater Trawl Survey (DE1010, EK60)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  2. NEFSC 2000 Atlantic Herring Acoustic/Midwater Trawl Survey (DE0008, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  3. NEFSC 2001 Atlantic Herring Acoustic/Midwater Trawl Survey (DE0109, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  4. NEFSC 2005 Atlantic Herring Acoustic/Midwater Trawl Survey (DE0512, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  5. NEFSC 2007 Atlantic Herring Acoustic/Midwater Trawl Survey (DE0710, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  6. NEFSC 2012 Atlantic Herring Acoustic/Midwater Trawl Survey (PC1206, EK60)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  7. NEFSC 1998 Atlantic Herring Acoustic/Midwater Trawl Survey (DE9809, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  8. NEFSC 2009 Atlantic Herring Acoustic/Midwater Trawl Survey (DE0910, EK500)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Atlantic herring acoustic survey is a fisheries independent survey that provides scientific data for Atlantic herring assessments in the U.S. mid-Atlantic...

  9. Exposure to parvalbumin allergen and aerosols among herring processing workers.

    Science.gov (United States)

    Dahlman-Höglund, Anna; Renström, Anne; Acevedo, Fernando; Andersson, Eva

    2013-10-01

    There are increasing reports of allergies and respiratory symptoms among workers in the fish processing industry, coinciding with an increasing use of high-pressure water in the processing plants. However, few studies have measured exposure in these work environments. The aim of this study was to characterize the occupational exposure of workers to herring antigen and to screen environmental factors at a herring (Clupea harengus) plant in which new and more encapsulated filleting machines had been installed. To assist in this, a method to assess airborne exposure to herring allergen was needed. Exposure to airborne herring antigen, mould spores, and endotoxin were measured during work. Antigen exposure was assessed using a newly developed sensitive (detection limit, 0.1 ng ml(-1)) rabbit polyclonal sandwich enzyme-linked immunosorbent assay against the major herring muscle protein allergen, parvalbumin. Aerosols were measured by mass concentration (DataRAM) and number of particles (Climet I-500). Personal geometric mean herring allergen exposure was 986 ng m(-3) at the old filleting workstations and 725 ng m(-3) at the new workstations (difference not significant). Outside the production room, the level was ~130 ng m(-3). Number of particles and mass concentration were both significantly lower around the new machines than around the old machines (P < 0.001 and P < 0.0001, respectively). The highest particle count was seen for the 0.3-0.5 μm fraction, with more than 400,000 particles per cubic metre air. Endotoxin concentration in the air varied between 3 and 92 EU m(-3), with the highest levels when the catch mainly contained herring that had eaten krill or seaweed. We developed a sensitive method to detect herring antigen. High exposure to herring antigen was measured during filleting work. The particles in the air around the fillet machines were mainly <0.5 μm and the newer encapsulated machines generated fewer particles. It is important to reduce occupational

  10. Association of myostatin variants with growth traits of Zhikong scallop ( Chlamys farreri)

    Science.gov (United States)

    Fu, Qiang; Guo, Huihui; Feng, Liying; Li, Xue; Zhang, Lingling; Wang, Shi; Hu, Xiaoli; Bao, Zhenmin

    2016-02-01

    Scallop is a popular sea food and an important aquaculture shellfish. Identification of genes and genetic variants relating to scallop growth could benefit high-yielding scallop breeding. Myostatin ( MSTN) is a conservative regulator of muscle growth, and has become one of the most important target genes for genetic improvement of the production of farmed animals. In this study, four single nucleotide polymorphisms (SNPs) were identified in the 5' flanking region of MSTN gene ( CfMSTN) in Zhikong scallop ( Chlamys farreri). The association of these SNPs with scallop growth traits, including shell length, shell height, body weight and striated muscle weight was analyzed. The SNP g-1162Gscallops showed significantly higher trait values than those of GT type, and the GG type individuals exhibited median values. On the contrary, significantly more CfMSTN transcripts were detected in the striated muscle of GT type scallops than in those of TT and GG type ones. Our results suggested that CfMSTN might regulate the scallop muscle growth negatively, and SNP g-1162Gscallop.

  11. 50 CFR 648.207 - Herring Research Set-Aside (RSA).

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Herring Research Set-Aside (RSA). 648.207... Measures for the Atlantic Herring Fishery § 648.207 Herring Research Set-Aside (RSA). (a) NMFS shall... authorized to utilize RSA, based on the selection criteria described in the RFP. (d) NMFS shall consider each...

  12. Pacific and Atlantic herring produce burst pulse sounds.

    Science.gov (United States)

    Wilson, Ben; Batty, Robert S; Dill, Lawrence M

    2004-02-07

    The commercial importance of Pacific and Atlantic herring (Clupea pallasii and Clupea harengus) has ensured that much of their biology has received attention. However, their sound production remains poorly studied. We describe the sounds made by captive wild-caught herring. Pacific herring produce distinctive bursts of pulses, termed Fast Repetitive Tick (FRT) sounds. These trains of broadband pulses (1.7-22 kHz) lasted between 0.6 s and 7.6 s. Most were produced at night; feeding regime did not affect their frequency, and fish produced FRT sounds without direct access to the air. Digestive gas or gulped air transfer to the swim bladder, therefore, do not appear to be responsible for FRT sound generation. Atlantic herring also produce FRT sounds, and video analysis showed an association with bubble expulsion from the anal duct region (i.e. from the gut or swim bladder). To the best of the authors' knowledge, sound production by such means has not previously been described. The function(s) of these sounds are unknown, but as the per capita rates of sound production by fish at higher densities were greater, social mediation appears likely. These sounds may have consequences for our understanding of herring behaviour and the effects of noise pollution.

  13. Little genetic variation in the oil sardine, Sardinella longiceps Val., from the western coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Menezes, M.R.

    Enzyme gene variability in the oil sardine, @iSardinella longiceps@@, from three localities along the western coast of India was studied by starch-gel electrophoresis. Out of 19 loci scored, no locus was polymorphic by the 95% criterion. Seven loci...

  14. The KwaZulu-Natal sardine run: shoal distribution in relation to ...

    African Journals Online (AJOL)

    The predator variables were highly significant (r2 = 0.48) with Cape gannets Morus capensis, followed by the sharks/gamefish and common dolphins Delphinus capensis, being most closely associated with sardine presence. Environmental variables were not as influential in the GLM models (r2 = 0.23), but some variables ...

  15. Effects of chitosan on the shelf life of marinated sardine (Sardina pilchardus fillets during refrigerated storage

    Directory of Open Access Journals (Sweden)

    Aygül Küçükgülmez

    2012-07-01

    Full Text Available This study was carried out to evaluate the effect of chitosan on chemical, colour, sensory and microbial changes of marinated sardine (Sardina pilchardus fillets. Marination solution consisted of 10% sodium chloride + 1% chitosan (dissolved in 3% acetic acid for the chitosan group, and 10% sodium chloride + 3% acetic acid solution for the control group. After the marination process, sardine fillets were packed and stored at 4ºC for 60 days. Thiobarbituric acid (TBA values were found to be lower in the chitosan group than the control group (PL*, a*, or b* values of marinated sardine fillets. According to sensory analysis, shelf life of the chitosan group was found to be ten days longer than that of the control group. Total bacteria count of two marinated groups was found to be less than 1 log CFU/g. This study concluded that sardine marination with the addition of chitosan can delay undesirable chemical changes, retard lipid oxidation, improve sensory attributes and extend the shelf life of the product during refrigerated storage.

  16. Effect of Neat Sardine Oil with Varies Blends on the Performance ...

    African Journals Online (AJOL)

    This study investigates the performance and emission characteristics of a diesel engine which is fuelled with neat sardine oil and diesel. A single cylinder four stroke diesel engine was used for the experiments at various load and speed of 1500 rpm. An AVL5 gas analyzer and a smoke meter were used for the ...

  17. Trends in the abundance and distribution of anchovy and sardine on ...

    African Journals Online (AJOL)

    main surveys are conducted annually, in winter to determine the strength of the year's recruitment and in summer to estimate the size of the adult stock. The results of ... The biomass of adult sardine, however, is better explained on the basis of the biomass of 2-year-old and older fish in the previous year's survey. Anchovy ...

  18. Transport of anchovy and sardine eggs and larvae from The western ...

    African Journals Online (AJOL)

    the frontal jet plays an important role in the transport of the early life history stages of anchovy and sardine, but that the position of such transport can vary between the 200 and 500-m isobaths. During October 1994, November 1994 and February 1995, the greatest concentrations of eggs corresponded with areas of strong ...

  19. Overview of the KwaZulu-Natal sardine run | van der Lingen | African ...

    African Journals Online (AJOL)

    This paper provides an introduction to, and overview of, the natural phenomenon known as the KwaZulu-Natal sardine run. Previous literature on this topic and hypotheses about the reasons why, and the mechanisms how, the run occurs are briefly synthesised and described. Papers contributing to this suite that detail more ...

  20. Paralytic shellfish toxins in the sea scallop Placopecten magellanicus on Georges Bank: Implications for an offshore roe-on and whole scallop fishery

    Science.gov (United States)

    DeGrasse, Stacey; Vanegas, Camilo; Conrad, Stephen

    2014-05-01

    To protect public health from the potential risk of paralytic shellfish poisoning (PSP) in sea scallops, Placopecten magellanicus, from the Atlantic offshore U.S. waters of Georges Bank, harvesting of roe-on or whole scallops is banned. Only adductor muscles may be sold if harvested from Georges Bank, Gulf of Maine, or the PSP closure areas as far west as 71° West Longitude. Given the limited toxicity data available for sea scallops from this region both prior to and subsequent to implementation of this management strategy, this study sought a more extensive spatial and temporal evaluation of sea scallop gonad and viscera toxicities that would inform management decisions related to the roe-on and whole scallop fishery. Both overall toxicity and toxin composition were measured for sea scallop gonads and viscera collected from 232 stations in 2007 and 23 in 2010. Overall toxicity was assessed using two screening methods: field-deployable Jellett Rapid Tests (JRT) and quantitative, laboratory-based receptor binding assays (in 2007). Additionally, a quantitative liquid chromatography with fluorescence detection (LC-FD) method was used to determine toxin composition and overall toxicity (in 2010). The at-sea qualitative JRT screening tool results, whereby a positive indicated the sample contained at least 40 μg saxitoxin (STX) equivalents 100 g-1, were often inconsistent with results obtained using the quantitative methods. Sea scallop viscera toxicity represented the majority of toxin load in the organism and was often in excess of the regulatory guidance level. Sea scallop gonads accounted for a small percentage of total toxicity, but at times reached unsafe levels. Toxin composition in both the gonads and viscera was dominated by STX and gonyautoxin 2/3, as has been reported in previous studies. No predictive indices of gonad toxicity were found. Results at this time do not support a roe-on or whole scallop fishery on Georges Bank. While access restrictions to

  1. Antioxidant activities and functional properties of protein and peptide fractions isolated from salted herring brine

    DEFF Research Database (Denmark)

    Taheri, Ali; Farvin, Sabeena; Jacobsen, Charlotte

    2014-01-01

    In the present study proteins isolated from herring brine, which is a by-product of marinated herring production were evaluated for their functional properties and antioxidant activity. Herring brine was collected from the local herring industry and proteins were precipitated by adjusting the p...... to delay iron catalyzed lipid oxidation in 5% fish oil in water emulsions and the 10–50kDa fraction was the best. These results show the potential of proteins and peptide fractions recovered from waste water from the herring industry as source of natural antioxidants for use in food products....

  2. 77 FR 28311 - Fisheries of the Northeastern United States; Atlantic Sea Scallop Fishery; Closure of the...

    Science.gov (United States)

    2012-05-14

    ...'s Scallop Plan Development Team (PDT) meeting on January 5, 2012, staff from the Northeast Fisheries... area rotation program for future years. New Information Regarding Current Scallop Biomass Levels in CAI... this area is essential for the future success of area rotation to maximize yield and economic benefits...

  3. 76 FR 40674 - Fisheries of the Exclusive Economic Zone Off Alaska; Scallops

    Science.gov (United States)

    2011-07-11

    ... accountability measures (AMs) to prevent overfishing in the target fishery for weathervane scallops. Implementing... statewide estimate of spawning biomass for weathervane scallops, the overfishing level (OFL) is specified as...-Stevens Act relative to preventing overfishing and establishing an ABC and ACL. The following is a summary...

  4. 77 FR 52 - Fisheries of the Northeastern United States; Atlantic Sea Scallop Fishery; Framework Adjustment 23

    Science.gov (United States)

    2012-01-03

    ... Fishery Management Plan, which was developed and adopted by the New England Fishery Management Council and... limited access general category Northern Gulf of Maine management program; and modify the scallop vessel monitoring system trip notification procedures to improve flexibility for the scallop fleet. DATES: Comments...

  5. Age and growth of the southeastern Brazilian sardine, Sardinella brasiliensis

    Directory of Open Access Journals (Sweden)

    Suzana Anita Saccardo

    1988-01-01

    Full Text Available Brazilian sardine datawere collected between September 1981 and August 1982 from the southeastern Brazilian waters. Otoliths were read using reflected ligth on the surface of the whole structure. Three methods were used to determine the time of ring formation, being the mean length per ring the best one. There are two annual rings clearly visible, one laid down in November/December, and the other one laid down in March/April. A method of age determination is described, being the maximum age 3 years.A idade da sardinha Sardinella brasiliensis foi determinada pelo exame de otólitos de exemplares provenientes de desembarques da pesca comercial e artesanal, na área compreendida entre 22ºS (Cabo Frio, RJ e 28ºS (Cabo de Santa Marta, Grande, SC, durante o período 1981-83. Este estudo e parte de um programa mais amplo de nominado "Programa Integrado de Estu dos Biológicos sobre Sardinha", desenvolvido na região sudeste do Brasil desde 1981, objetivando conhecer as variações dos parâmetros biológicos da espécie, e fornecer subsídios a avaliação pesqueira do recurso. Foram contados os anéis translúcidos dos otolitos, colocados inteiros em recipientes de fundo preto, imersos em álcool 70% e iluminados com luz incidente sob estereomicroscópio binocular. Além das contagens, foram efetuadas medidas das distancias do núcleo do otolito ao bordo, e do núcleo a cada anél translúcido, bem como observações da natureza do bordo. Foram utilizados três métodos para determinar a época e periodicidade de formação dos anéis. Os parâmetros de crescimento L, K e t0 foram estimados, e as curvas ajustadas usando-se a equação de von Bertalanffy. Os resultados mostraram que os otolitos possuem de 0 a 7 anéis, sendo formados dois anéis por ano: um entre novembro-dezembro, e outro entre março-abril. Estas épocas estão intimamente ligadas ao período de reprodução. Descreveu-se um método para a determinação da idade de sardinha, tendo

  6. Distribution of juvenile Pacific herring relative to environmental and geospatial factors in Prince William Sound, Alaska

    Science.gov (United States)

    Lewandoski, Sean; Bishop, Mary Anne

    2018-01-01

    Documenting distribution patterns of juvenile Pacific herring (Clupea pallasii) can clarify habitat preferences and provide insight into ecological factors influencing early life survival. However, few analyses relating juvenile Pacific herring density to habitat characteristics have been conducted. We sampled age-0 Pacific herring in nine bays and fjords distributed throughout Alaska's Prince William Sound during November over a 3-year period (2013-2015) and investigated associations between catch rate and habitat covariates using generalized linear mixed models. Our results indicated that the night-time distribution of age-0 Pacific herring in the pelagic environment was influenced by proximity to eelgrass (Zostera marina) beds, salinity, and water depth. Age-0 Pacific herring catch rate was negatively associated with tow depth, with herring favoring shallower water across the range of depths sampled (7.2-35.4 m). In addition, Pacific herring distribution was positively associated with fresher water within the sampled salinity gradient (24.1-32.3 psu) and proximity to eelgrass beds. Seasonal changes in juvenile Pacific herring distribution were investigated by sampling one bay over a seven month period (October-April). Age-0 Pacific herring tended to remain in the inner bay region throughout the seven months, while age-1 Pacific herring had shifted from the inner to the outer bay by spring (March-April). Additionally, catch rate of age-0 Pacific herring in areas where ice breakup had just occurred was higher than in open water, suggesting that age-0 herring preferentially select ice-covered habitats when available. Based on our results we recommend that habitat preferences of age-0 Pacific herring should be considered in the development of Pacific herring year-class strength indices from catch data.

  7. Meat Composition and Quality Assessment of King Scallops (Pecten maximus) and Frozen Atlantic Sea Scallops (Placopecten magellanicus) on a Retail Level

    Science.gov (United States)

    Manthey-Karl, Monika; Lehmann, Ines; Ostermeyer, Ute; Rehbein, Hartmut; Schröder, Ute

    2015-01-01

    An enlarged range of scallop products on the market allows the consumer to buy lower priced alternatives, which often raises the question of quality and control. Frozen meat of king scallops (Pecten maximus) and Atlantic sea scallops (Placopecten magellanicus) were purchased on the German market and compared with fresh shell-on king scallops of various origin. The approximate composition, inclusive citric acid and phosphates, minerals, free amino acids (FAA) and fatty acid profiles were examined in the muscle to identify changes as a result of processing. The FAA glycine and taurine as well the fatty acids 20:5n-3 (EPA) and 22:6n-3 (DHA) were the most abundant, but were reduced in processed samples. Di- and triphosphate contents were not detectable (additives. Labelling does not readily allow the consumer to recognize the extent of processing effects. PMID:28231221

  8. Influence of feeding graded levels of canned sardines on the inflammatory markers and tissue fatty acid composition of Wistar rats.

    Science.gov (United States)

    Rodrigues, Pedro O; Martins, Susana V; Lopes, Paula A; Ramos, Cristina; Miguéis, Samuel; Alfaia, Cristina M; Pinto, Rui M A; Rolo, Eva A; Bispo, Paulo; Batista, Irineu; Bandarra, Narcisa M; Prates, José A M

    2014-08-14

    Canned sardines are a ready-to-use fish product with excellent nutritional properties owing to its high n-3 long-chain PUFA content, mainly EPA (20 : 5n-3) and DHA (22 : 6n-3). The present study aimed to assess the effect of two dosages of canned sardines, recommended for the primary and secondary prevention of human CVD, on the inflammatory marker concentrations and fatty acid composition of erythrocytes and key metabolic tissues (liver, muscle, adipose tissue and brain) in the rat model. Wistar rats were fed a diet containing 11 % (w/w) of canned sardines (low-sardine (LS) diet) and a diet containing 22 % (w/w) of canned sardines (high-sardine (HS) diet) for 10 weeks. Daily food intake, weight gain, and organ and final body weights were not affected by the dietary treatments. The concentrations of total cholesterol, HDL-cholesterol and LDL-cholesterol decreased in both the LS and HS groups, while those of alanine aminotransferase and adiponectin increased. The concentrations of IL-1β increased only with the highest dosage of sardine. The dose-dependent influence of the graded levels of EPA+DHA was tissue specific. Compared with that of other tissues and erythrocytes, the fatty acid composition of the brain was less affected by the canned sardine-supplemented diets. In contrast, the retroperitoneal adipose tissue was highly responsive. The deposition ratios of EPA and DHA indicated that the LS diet was optimal for DHA deposition across the tissues, except in the retroperitoneal adipose tissue. Taken together, our findings indicate that a LS diet positively affects plasma lipid profiles and inflammatory mediators, whereas a HS diet has contradictory effects on IL-1β, which, in turn, is not associated with variations in the concentrations of other pro-inflammatory cytokines. This finding requires further investigation and pathophysiological understanding.

  9. Evaluating fishermen's conservation attitudes and local ecological knowledge of the European sardine (Sardina pilchardus), Peniche, Portugal.

    Science.gov (United States)

    Braga, Heitor O; Azeiteiro, Ulisses M; Oliveira, Henrique M F; Pardal, Miguel A

    2017-05-05

    European sardines are an important fishing resource in the North Atlantic. Recognized for its great commercial and economic value in southern Europe, this resource currently has low stock indices. From this perspective, fishers' local ecological knowledge (LEK) is appreciated as an auxiliary tool in the management of sardines in this region. Our goal is to evaluate the LEK and attitudes towards the conservation of Sardina pilchardus in the typical fishing village of Peniche, Portugal. From June to September 2016, we carried out 87 semi-structured interviews. The four main points of the interviews were interviewee profile, fishing structure, fishermen's LEK and attitudes towards sardine conservation. The interviews were qualitatively and quantitatively analyzed using a 3-point Likert scale. An LEK index and an attitude index were generated. Comparison analyses and correlations were made between the indices and variables of the interviewee profile and the fishing structure. The mean LEK index was 0.55 and was classified as moderate. The attitudes index in relation to conservation was 0.76 and was classified as positive. This index had a positive and significant correlation with the LEK index and a significant negative correlation with the fishermen's age. When the LEK index was compared with the educational level, significant differences were observed only between class A and class C. The result showed that the differences in the attitudes index were statistically significant when the three educational classes were compared. The fishermen of Peniche in Portugal present moderate informal knowledge about the biology and ecology of sardines. Attitudes towards conservation were predominantly positive. Fishermen with greater LEK, with a higher educational level and at a younger age presented more positive attitudes in relation to environmental conservation issues in the present case of the sardine population. The LEK is not necessarily related to the educational level of

  10. Review of Ruegen herring larvae survey project 2011

    NARCIS (Netherlands)

    Dickey-Collas, M.; Nash, R.D.M.

    2011-01-01

    At the request of the Johann Heinrich von Thünen Institut (vTI), IOR Rostock, a return review of the German Rügen herring larval survey (RHLS) project took place on the 24th to 26th January 2011, following on from a similar review in November 2006. Mark Dickey-Collas (Wageningen IMARES) and Richard

  11. Meat Composition and Quality Assessment of King Scallops (Pecten maximus and Frozen Atlantic Sea Scallops (Placopecten magellanicus on a Retail Level

    Directory of Open Access Journals (Sweden)

    Monika Manthey-Karl

    2015-09-01

    Full Text Available An enlarged range of scallop products on the market allows the consumer to buy lower priced alternatives, which often raises the question of quality and control. Frozen meat of king scallops (Pecten maximus and Atlantic sea scallops (Placopecten magellanicus were purchased on the German market and compared with fresh shell-on king scallops of various origin. The approximate composition, inclusive citric acid and phosphates, minerals, free amino acids (FAA and fatty acid profiles were examined in the muscle to identify changes as a result of processing. The FAA glycine and taurine as well the fatty acids 20:5n-3 (EPA and 22:6n-3 (DHA were the most abundant, but were reduced in processed samples. Di- and triphosphate contents were not detectable (<0.01 g·kg−1 in untreated meats. Most frozen scallop products contained added citrates and polyphosphates and had distinctly higher water contents (up to 89% and an increased moisture to protein ratio (M/P (up to 9 compared with the fresh king scallops (78%, M/P < 5. Labelling of species, verified by PCR-based DNA analysis, and ingredients were not correct in each case. Overall results indicated no relevant differences in mineral content, except high sodium contents, resulting from additives. Labelling does not readily allow the consumer to recognize the extent of processing effects.

  12. Technology of puddings made of Pacific herring milt

    Directory of Open Access Journals (Sweden)

    Dement'eva N. V.

    2016-09-01

    Full Text Available The technology of culinary products (puddings of finely ground Pacific herring milt has been experimentally proved. The standard physics and chemical, organoleptic, microbiological methods for food analysis have been used. The main raw material is frozen milt of Pacific herring. Fresh eggs, drinking cream, berries (cranberries, raspberries, strawberries, etc. frozen and fresh, sugar, and soda have been used as additional materials. The ratio of main components in production of puddings from Pacific herring milt is: milt 35–40 %, cream 20–25 %, eggs 15–22 %. The recipes of sweet puddings of milt with the addition of berries have been developed. The effect of duration of whipping protein mass on puddings' organoleptic parameters has been found experimentally. The recommended duration of whipping is from 3 to 5 minutes, this increases the amount of pudding mass lifting after the whipping of not less than 40 % of the original and provides the necessary structure in the finished product after heat treatment. The rational heat treatment of puddings' baking has been substantiated – at the temperature of 150 °C, with the duration of heat treatment for 20 minutes, which provides high organoleptic characteristics and safety of the finished product. The recommended shelf life of the finished product of not more than 72 hours at the temperature of 2–6 °C has been determined. On the basis of the research the technology of new kinds of culinary fish products – puddings of Pacific herring milt allowing obtain culinary products with high organoleptic characteristics – has been developed. The unique chemical composition combined (milk, eggs, cream and berries provides a basis for classification of new products as functional food. Additional involvement in food production little used milt of herring species of fish enables to expand the range of fish culinary products and increase the rate of food use of water origin raw material.

  13. Production of biodiesel by enzymatic transesterification of waste sardine oil and evaluation of its engine performance

    Directory of Open Access Journals (Sweden)

    A. Arumugam

    2017-12-01

    Full Text Available Waste sardine oil, a byproduct of fish industry, was employed as a low cost feedstock for biodiesel production. It has relatively high free fatty acid (FFA content (32 mg KOH/g of oil. Lipase enzyme immobilized on activated carbon was used as the catalyst for the transesterification reaction. Process variables viz. reaction temperature, water content and oil to methanol molar ratio were optimized. Optimum methanol to oil molar ratio, water content and temperature were found to be 9:1, 10 v/v% and 30 °C respectively. Reusability of immobilized lipase was studied and it was found after 5 cycles of reuse there was about 13% drop in FAME yield. Engine performance of the produced biodiesel was studied in a Variable Compression Engine and the results confirm that waste sardine oil is a potential alternate and low-cost feedstock for biodiesel production.

  14. Production of biodiesel by enzymatic transesterification of waste sardine oil and evaluation of its engine performance.

    Science.gov (United States)

    Arumugam, A; Ponnusami, V

    2017-12-01

    Waste sardine oil, a byproduct of fish industry, was employed as a low cost feedstock for biodiesel production. It has relatively high free fatty acid (FFA) content (32 mg KOH/g of oil). Lipase enzyme immobilized on activated carbon was used as the catalyst for the transesterification reaction. Process variables viz. reaction temperature, water content and oil to methanol molar ratio were optimized. Optimum methanol to oil molar ratio, water content and temperature were found to be 9:1, 10 v/v% and 30 °C respectively. Reusability of immobilized lipase was studied and it was found after 5 cycles of reuse there was about 13% drop in FAME yield. Engine performance of the produced biodiesel was studied in a Variable Compression Engine and the results confirm that waste sardine oil is a potential alternate and low-cost feedstock for biodiesel production.

  15. The effect of protected sardine fish oil as feed supplement on ruminal fermentation

    Science.gov (United States)

    Pramono, A.; Widayati, D. T.; Handayanta, E.

    2018-03-01

    The research aims to evaluate the influence of protected sardine fish oil as feed supplement on ruminal fermentation (pH rumen fluid, ammonia concentration and volatile fatty acids production in the rumen). Protected feed supplement was produced from sardine fish oil and soybean meal, through two protection methods, they were saponification and microencapsulation. The experiment consists of two treatments i.e. P0: basal diet (control) and P1: basal diet + 3 % protected feed supplement. Each treatment was repeated 10 times. The kinetics observation of the pH rumen fluid, ammonia concentration and volatile fatty acids production were performed at incubation times 0, 2, 4 and 6 hours respectively. Data were analyzed using independent samples t-test. Results in cow with protected feed supplement showed that kinetics of pH rumen fluid: 7.23; 7.13; 6.90 and 6.76 respectively; ruminal ammonia concentration: 26.70; 31.06; 19.75 and 15.52 respectively; and volatile fatty acids production: 22.75; 26.08; 29.19 and 25.79 respectively. The results could be concluded that the effect of supplementation of protected sardine fish oil have an optimal of pH rumen fluid, ammonia concentration, and volatile fatty acids production so it did not interfere the ruminal fermentation in the rumen.

  16. Does predation by grey seals (Halichoerus grypus) affect Bothnian Sea herring stock estimates?

    DEFF Research Database (Denmark)

    Gårdmark, Anna; Östman, Örjan; Nielsen, Anders

    2012-01-01

    when accounting for seal predation, this did not change the conclusions about drivers of herring dynamics. Accounting for grey seal predation is important for abundance estimates of old herring, but currently not for SSB estimates, given the great uncertainties in the standard assessment. The grey seal...... fivefold since 1985. Its main prey, herring (Clupea harrengus), is a key species for fisheries in the region. Yet, current stock assessments assume constant natural mortality, leading to a risk of biased stock estimates with increasing predation and misleading analyses of herring population dynamics. We...... estimated grey seal predation from diet data and reanalysed herring spawning stock biomass (SSB) during 1973–2009. Accounting for predation increased the herring SSB 16% (maximum 19%), but this was within the confidence intervals when ignoring predation. Although mortality in older individuals was inflated...

  17. Textural and biochemical changes during ripening of old-fashioned salted herrings

    DEFF Research Database (Denmark)

    Christensen, Mette; Andersen, Eva; Christensen, Line

    2011-01-01

    the ripening period could be explained by free-radical-induced cross-linking of myosin and the formation of aggregates. In addition, degradation of these aggregates correlated with the decrease in hardness observed at 371 days. CONCLUSIONS: Texture changes during ripening of salted herrings can be explained...... of salted herrings. The aim of this study was to measure the texture changes during ripening using two differentmethods and to correlate the texture changeswith brine composition andwith biochemical modifications at themolecular level. RESULTS: During ripening (up to 151 days), hardness was higher in salted...... herrings compared to raw herrings, irrespective of the brine composition. However, the increase in hardness of herring prepared with extra brine occurred later. After prolonged storage (371 days), hardness was found for both batches to decrease to the level of raw herring. The increase in hardness during...

  18. Life history of lake herring of Green Bay, Lake Michigan

    Science.gov (United States)

    Smith, Stanford H.

    1956-01-01

    Although the lake herring has been an important contributor to the commercial fish production of Green Bay, little has been known about it. This study is based on field observations and data from about 6,500 lake herring collected over the period 1948 to 1952. Relatively nonselective commercial pound nets were a primary source of material for the study of age and growth. Commercial and experimental gill nets were used to obtain data on gear selectivity and vertical distribution. Scales were employed to investigate age and growth. Age group IV normally dominated commercial catches during the first half of the calendar year and age group III the last half. At these ages the fish averaged about 10.5 inches in length. The season's growth started in May, was most rapid in July, and terminated near the end of October. The sexes grew at the same rate. Selectivity of fishing gear was found to influence the estimation of growth. Geographical and annual differences in growth are shown. Factors that might contribute to discrepancies in calculated growth are evaluated. Possible real and apparent causes of growth compensation are given. The relation between length and weight is shown to vary with sex, season, year, and method of capture. Females were relatively more plentiful in commercial catches in February than in May through December. The percentage of females decreased with increase in age in pound-net catches but increased with age in gill-net samples. Within a year class the percentage of females decreased with increase in age. Most Green Bay lake herring mature during their second or third year of life. They are pelagic spawners with most intensive spawning over shallow areas. Spawning takes place between mid-November and mid-December, and eggs hatch in April and May. Lake herring ovaries contained from 3,500 to 11,200 eggs (averaged 6,375). Progress of spawning by age, sex, and length is given. Lake herring were distributed at all depths in Green Bay in early May, were

  19. Migration patterns of post-spawning Pacific herring in a subarctic sound

    Science.gov (United States)

    Bishop, Mary Anne; Eiler, John H.

    2018-01-01

    Understanding the distribution of Pacific herring (Clupea pallasii) can be challenging because spawning, feeding and overwintering may take place in different areas separated by 1000s of kilometers. Along the northern Gulf of Alaska, Pacific herring movements after spring spawning are largely unknown. During the fall and spring, herring have been seen moving from the Gulf of Alaska into Prince William Sound, a large embayment, suggesting that fish spawning in the Sound migrate out into the Gulf of Alaska. We acoustic-tagged 69 adult herring on spawning grounds in Prince William Sound during April 2013 to determine seasonal migratory patterns. We monitored departures from the spawning grounds as well as herring arrivals and movements between the major entrances connecting Prince William Sound and the Gulf of Alaska. Departures of herring from the spawning grounds coincided with cessation of major spawning events in the immediate area. After spawning, 43 of 69 tagged herring (62%) moved to the entrances of Prince William Sound over a span of 104 d, although most fish arrived within 10 d of their departure from the spawning grounds. A large proportion remained in these areas until mid-June, most likely foraging on the seasonal bloom of large, Neocalanus copepods. Pulses of tagged herring detected during September and October at Montague Strait suggest that some herring returned from the Gulf of Alaska. Intermittent detections at Montague Strait and the Port Bainbridge passages from September through early January (when the transmitters expired) indicate that herring schools are highly mobile and are overwintering in this area. The pattern of detections at the entrances to Prince William Sound suggest that some herring remain in the Gulf of Alaska until late winter. The results of this study confirm the connectivity between local herring stocks in Prince William Sound and the Gulf of Alaska.

  20. Energetic Cost of Ichthyophonus Infection in Juvenile Pacific Herring (Clupea pallasii)

    OpenAIRE

    Johanna J. Vollenweider; Jake L. Gregg; Ron A. Heintz; Paul K. Hershberger

    2011-01-01

    The energetic costs of fasting and Ichthyophonus infection were measured in juvenile Pacific herring (Clupea pallasii) in a lab setting at three temperatures. Infected herring incurred significant energetic costs, the magnitude of which depended on fish condition at the time of infection (fat versus lean). Herring that were fed continually and were in relatively good condition at the time of infection (fat) never stored lipid despite ad libitum feeding. In feeding herrin...

  1. Sensory properties of marinated herring (Clupea harengus) processed from raw material from commercial landings

    DEFF Research Database (Denmark)

    Nielsen, Durita; Hyldig, Grethe; Nielsen, Jette

    2005-01-01

    Sensory properties of marinated herring processed from raw material from Danish commercial catches were described and related to fishing season and biological, chemical and functional properties. Herring was caught on five cruises and stored on board in tanks or ice. The sensory profile of marina......Sensory properties of marinated herring processed from raw material from Danish commercial catches were described and related to fishing season and biological, chemical and functional properties. Herring was caught on five cruises and stored on board in tanks or ice. The sensory profile...

  2. Baltic herring fisheries management: stakeholder views to frame the problem

    DEFF Research Database (Denmark)

    Haapasaari, Päivi Elisabet; Mäntyniemi, Samu; Kuikka, Sakari

    2012-01-01

    Comprehensive problem framing that includes different perspectives is essential for holistic understanding of complex problems and as the first step in building models. We involved five stakeholders to frame the management problem of the Central Baltic herring fishery. By using the Bayesian belief...... nongovernmental organization included markets and fishing industry influences. Management measures were considered to have a relatively small impact on the development of the herring stock; their impact on socioeconomic objectives was greater. Overall, the framings by these stakeholders propose a focus...... networks (BBNs) approach, the views of the stakeholders were built into graphical influence diagrams representing variables and their dependencies. The views of the scientists involved concentrated on biological concerns, whereas the fisher, the manager, and the representative of an environmental...

  3. Interacting trophic forcing and the population dynamics of herring

    DEFF Research Database (Denmark)

    Lindegren, Martin; Ostman, Orjan; Gardmark, Anna

    2011-01-01

    -up nor top-down, but rather through multiple external and internal drivers. While in many studies single drivers have been identified, potential synergies of multiple factors, as well as their relative importance in regulating population dynamics of small pelagic fish, is a largely unresolved issue....... Using a statistical, age-structured modeling approach, we demonstrate the relative importance and influence of bottom-up (e.g., climate, zooplankton availability) and top-down (i.e., fishing and predation) factors on the population dynamics of Bothnian Sea herring (Clupea harengus) throughout its life...... cycle. Our results indicate significant bottom-up effects of zooplankton and interspecific competition from sprat (Sprattus sprattus), particularly on younger age classes of herring. Although top-down forcing through fishing and predation by grey seals (Halichoerus grypus) and Atlantic cod (Gadus morhua...

  4. The high diversity of arsenolipids in herring fillet (Clupea harengus).

    Science.gov (United States)

    Lischka, S; Arroyo-Abad, U; Mattusch, J; Kühn, A; Piechotta, Ch

    2013-06-15

    Arsenolipids represent a relevant step in the biosynthesis of organoarsenicals from inorganic arsenic compounds. Their fate after human consumption is still uncertain. By means of a HPLC-ICP-MS/ESI-Q-TOF-MS method, 16 lipid soluble arsenic compounds, including seven formerly unknown organoarsenicals, have been identified in commercial herring fillet. The structural assignment was done by exact mass and high resolution MS/MS data. This is the first identification of arsenolipids in herring (Clupea harengus). They contribute with (3.6±0.2) mg kg(-1) arsenic to 62.3% of the total arsenic content of (5.7±0.3) mg of arsenic per kg dry mass. Current studies indicate that a metabolization by humans to cancerous dimethylarsinic acid is very likely. The presented results are highly relevant as herring is a very popular food fish species in Europe. Moreover, the screening of different fish species revealed that arsenolipids are more widespread than previously assumed. Copyright © 2013 Elsevier B.V. All rights reserved.

  5. Food web changes under ocean acidification promote herring larvae survival.

    Science.gov (United States)

    Sswat, Michael; Stiasny, Martina H; Taucher, Jan; Algueró-Muñiz, Maria; Bach, Lennart T; Jutfelt, Fredrik; Riebesell, Ulf; Clemmesen, Catriona

    2018-05-01

    Ocean acidification-the decrease in seawater pH due to rising CO 2 concentrations-has been shown to lower survival in early life stages of fish and, as a consequence, the recruitment of populations including commercially important species. To date, ocean-acidification studies with fish larvae have focused on the direct physiological impacts of elevated CO 2 , but largely ignored the potential effects of ocean acidification on food web interactions. In an in situ mesocosm study on Atlantic herring (Clupea harengus) larvae as top predators in a pelagic food web, we account for indirect CO 2 effects on larval survival mediated by changes in food availability. The community was exposed to projected end-of-the-century CO 2 conditions (~760 µatm pCO 2 ) over a period of 113 days. In contrast with laboratory studies that reported a decrease in fish survival, the survival of the herring larvae in situ was significantly enhanced by 19 ± 2%. Analysis of the plankton community dynamics suggested that the herring larvae benefitted from a CO 2 -stimulated increase in primary production. Such indirect effects may counteract the possible direct negative effects of ocean acidification on the survival of fish early life stages. These findings emphasize the need to assess the food web effects of ocean acidification on fish larvae before we can predict even the sign of change in fish recruitment in a high-CO 2 ocean.

  6. Passive immunization of Pacific herring against viral hemorrhagic septicemia.

    Science.gov (United States)

    Hershberger, P.K.; Gregg, J.L.; Grady, C.A.; LaPatra, S.E.; Winton, J.R.

    2011-01-01

    The plasma of Pacific herring Clupea pallasii that survived laboratory-induced viral hemorrhagic septicemia (VHS) epizootics contained humoral substances that, when injected into naive animals, conferred passive immunity against the disease. Among groups exposed to viral hemorrhagic septicemia virus (VHSV), injection of donor plasma from VHS survivors resulted in significantly greater survival (50%) and significantly lower tissue titers (1.5 x 10(5) plaque-forming units [PFU]/g) than the injection of plasma from VHSV-naive donors (6% survival; 3.7 x 10(6) PFU/g). Additionally, the magnitude of the protective immune response increased during the postexposure period; plasma that was collected from survivors at 123 d postexposure (931 degree-days) provided greater protection than plasma collected from survivors at 60 d postexposure (409 degree-days). These results provide proof of concept that the VHSV exposure history of Pacific herring populations can be determined post hoc; furthermore, the results can be used as the foundation for developing additional high-throughput diagnostic techniques that may be effective at quantifying herd immunity and forecasting the potential for future VHS epizootics in populations of wild Pacific herring.

  7. Scalloped Implant-Abutment Connection Compared to Conventional Flat Implant-Abutment Connection

    DEFF Research Database (Denmark)

    Starch-Jensen, Thomas; Christensen, Ann-Eva; Lorenzen, Henning

    2017-01-01

    OBJECTIVES: The objective was to test the hypothesis of no difference in implant treatment outcome after installation of implants with a scalloped implant-abutment connection compared to a flat implant-abutment connection. MATERIAL AND METHODS: A MEDLINE (PubMed), Embase and Cochrane library search...... of suprastructures has never been compared within the same study. High implant survival rate was reported in all the included studies. Significantly more peri-implant marginal bone loss, higher probing depth score, bleeding score and gingival score was observed around implants with a scalloped implant-abutment...... loss around implants with a scalloped implant-abutment connection. CONCLUSIONS: A scalloped implant-abutment connection seems to be associated with higher peri-implant marginal bone loss compared to a flat implant-abutment connection. Therefore, the hypothesis of the present systematic review must...

  8. NODC Standard Format Herring Survey Population Density and Distribution (F057) Data (1976-1977) (NODC Accession 0014189)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The data type contains data from aircraft surveys of herring schools. These data were collected to provide information on herring population density and...

  9. High‐resolution acoustic indices of Atlantic herring (Clupea harengus) paving the way for inclusion of migration patterns in management considerations of herring in ICES Divisions IIIa and SD 22‐24

    DEFF Research Database (Denmark)

    Stæhr, Karl-Johan; Worsøe Clausen, Lotte

    2012-01-01

    Herring catches in the Western Baltic, Kattegat and Skagerrak consist of a mixture of stocks; the two predominant stocks are the spring‐spawning Western Baltic herring stock and the North Sea autumn‐spawning herring (NSAS), and the mixing follows an age‐ and season‐dependent pattern with high var...

  10. Production and characterization of refined oils obtained from Indian oil sardine (Sardinella longiceps).

    Science.gov (United States)

    Chakraborty, Kajal; Joseph, Deepu

    2015-01-28

    Crude Sardinella longiceps oil was refined in different stages such as degumming, neutralization, bleaching, and deodorization. The efficiency of these processes was evaluated on the basis of free fatty acid (FFA), peroxide (PV), p-anisidine (pAV), total oxidation (TOTOX), thiobarbituric acid reactive species (TBARS) values, Lovibond CIE-L*a*b* color analyses, and (1)H NMR or GC-MS experiments. The utilities of NMR-based proton signal characteristics as new analytical tools to understand the signature peaks and relative abundance of different fatty acids and monitoring the refining process of fish oil have been demonstrated. Phosphoric acid (1%) was found to be an effective degumming reagent to obtain oil with the lowest FFA, PV, pAV, TOTOX, and TBARS values and highest color reduction. Significant reduction in the contents of hydrocarbon functionalities as shown by the decrease in proton integral in the characteristic (1)H NMR region was demonstrated by using 1% H3PO4 during the course of the degumming process. A combination (1.25:3.75%) of activated charcoal and Fuller's earth at 3% concentration for a stirring time of 40 min was found to be effective in bleaching the sardine oil. This study demonstrated that unfavorable odor-causing components, particularly low molecular weight carbonyl compounds, could successfully be removed by the refining process. The alkane-dienals/alkanes, which cause unfavorable fishy odors, were successfully removed by distillation (100 °C) under vacuum with aqueous acetic acid solution (0.25 N) to obtain greater quality of refined sardine oil, a rich source of essential fatty acids and improved oxidative stability. The present study demonstrated that the four-stage refinement process of sardine oil resulted in a significant improvement in quality characteristics and nutritional values, particularly n-3 PUFAs, with improved fish oil characteristics for use in the pharmaceutical and functional food industries.

  11. Effect of natural antioxidants on the quality of frozen sardine fillets (Sardina pilchardus

    Directory of Open Access Journals (Sweden)

    Rui Manuel Maneta Ganhão

    2014-06-01

    Full Text Available The use of new technologies / processes provides different benefits on seafood sector, namely by enhanced the shelf-life of fish products with high polyunsaturated fatty acid composition. This is particularly critical during some months of the year when fish (e.g. sardines are larger supply than demand by consumers. The aim of this study was to performed the optimization process of adding natural antioxidants on frozen sardine fillets and evaluated the effect of addition of two natural antioxidants on the lipid oxidation process during storage at -200C. To evaluate the effect of the addition of antioxidants in sardine fillets, the product matrix was chemical characterized, including by the evaluation of the fatty acid profile. The lipid oxidation process was followed by the primary products (peroxide value and secondary products (TBARS quantification, and also by the analysis of the sensory changes (instrumental assessment of color. Finally, it was done a hedonic preference test to know the opinion of consumers about the frozen fillets with and without antioxidant. The natural antioxidants used, tocopherol (54 mg/fillet and tocopherols (15 mg/fillet with rosemary extract (6.5 mg/fillet inhibited or retarded the oxidation over time of storage when compared with the control samples, that had advanced oxidation process. There was not statistically differences among the fillets treated with tocopherol in the presence and absence of rosemary extract. However, our laboratorial results shown slight tendency for the view that the antioxidant tocopherols and rosemary extract had a greater effect on the oxidative stability of fillets. This observation is in line with results of the hedonic test consumer preference. The use of natural antioxidants is an inexpensive solution which allows the complete use of products with less waste of fish products and with a great need for disposal. At the same time meets the consumer´s requirements.

  12. Sardine (Sardina pilchardus) larval dispersal in the Iberian upwelling system, using coupled biophysical techniques

    Science.gov (United States)

    Santos, A. M. P.; Nieblas, A.-E.; Verley, P.; Teles-Machado, A.; Bonhommeau, S.; Lett, C.; Garrido, S.; Peliz, A.

    2018-03-01

    The European sardine (Sardina pilchardus) is the most important small pelagic fishery of the Western Iberia Upwelling Ecosystem (WIUE). Recently, recruitment of this species has declined due to changing environmental conditions. Furthermore, controversies exist regarding its population structure with barriers thought to exist between the Atlantic-Iberian Peninsula, Northern Africa, and the Mediterranean. Few studies have investigated the transport and dispersal of sardine eggs and larvae off Iberia and the subsequent impact on larval recruitment variability. Here, we examine these issues using a Regional Ocean Modeling System climatology (1989-2008) coupled to the Lagrangian transport model, Ichthyop. Using biological parameters from the literature, we conduct simulations that investigate the effects of spawning patchiness, diel vertical migration behaviors, and egg buoyancy on the transport and recruitment of virtual sardine ichthyoplankton on the continental shelf. We find that release area, release depth, and month of release all significantly affect recruitment. Patchiness has no effect and diel vertical migration causes slightly lower recruitment. Egg buoyancy effects are significant and act similarly to depth of release. As with other studies, we find that recruitment peaks vary by latitude, explained here by the seasonal variability of offshore transport. We find weak, continuous alongshore transport between release areas, though a large proportion of simulated ichthyoplankton transport north to the Cantabrian coast (up to 27%). We also show low level transport into Morocco (up to 1%) and the Mediterranean (up to 8%). The high proportion of local retention and low but consistent alongshore transport supports the idea of a series of metapopulations along this coast.

  13. 77 FR 10978 - Fisheries of the Northeastern United States; Atlantic Herring Fishery; Adjustment to 2012 Annual...

    Science.gov (United States)

    2012-02-24

    ... to prevent overfishing. DATES: Effective February 24, 2012, through December 31, 2012. ADDRESSES...). Although herring is not overfished and is not experiencing overfishing, the herring annual acceptable... level that prevents overfishing. The sub-ACLs overages in 2010 did not result in overfishing, therefore...

  14. 75 FR 20550 - Fisheries of the Northeastern United States; Atlantic Herring Fishery; Specifications

    Science.gov (United States)

    2010-04-20

    ... time. Additionally, the SSC recommended that a new herring benchmark stock assessment is needed to... MSY . A new benchmark stock assessment is needed to address the inconsistency between long-term... herring stock complex is a transboundary stock and is found in both U.S and Canadian waters. As such, the...

  15. 75 FR 48874 - Fisheries of the Northeastern United States; Atlantic Herring Fishery; Specifications

    Science.gov (United States)

    2010-08-12

    ... until a new benchmark stock assessment can be conducted. They cited concern about the high level of... additional data to assess the resource as it prepares for the next benchmark stock assessment in 2012. They... role of herring as a forage species, past declines in the New England herring stock, and concern that...

  16. Outlier loci detect intraspecific biodiversity amongst spring and autumn spawning herring across local scales

    DEFF Research Database (Denmark)

    Bekkevold, Dorte; Gross, Riho; Arula, Timo

    2016-01-01

    Herring, Clupea harengus, is one of the ecologically and commercially most important species in European northern seas, where two distinct ecotypes have been described based on spawning time; spring and autumn. To date, it is unknown if these spring and autumn spawning herring constitute genetica...... of these co-occurring ecotypes to meet requirements for sustainable exploitation and ensure optimal livelihood for coastal communities....

  17. Recovery of biomolecules from marinated herring (Clupea harengus) brine using ultrafiltration through ceramic membranes

    DEFF Research Database (Denmark)

    Gringer, Nina; Hosseini, Seyed Vali; Svendsen, Tore

    2015-01-01

    Marinated herring processing brines, which are usually discarded, are rich in salt, protein, non-protein nitrogen, iron, fatty acids, antioxidant and even possess enzymatic activity. This study investigated the performance of ceramic ultrafiltration of two herring spice brines with a major focus...

  18. Prey selection by North Sea herring (Clupea harengus) with special reference to fish eggs

    NARCIS (Netherlands)

    Segers, F.H.I.D.; Dickey-Collas, M.; Rijnsdorp, A.D.

    2007-01-01

    The herring stock in the North Sea in recent years has recovered to a relatively high biomass, and here we investigate prey selection of individual North Sea herring when population numbers are high. The diet composition, and specifically pelagic fish eggs, was investigated in February 2004. Samples

  19. In situ activity of chymotrypsin in sugar-salted herring during cold storage

    DEFF Research Database (Denmark)

    Engvang, K.L.; Nielsen, Henrik Hauch

    2000-01-01

    In sifts activity of intestinal chymotrypsin in sugar-salted whole herring during cold storage was evaluated by analysing changes in the low-molecular-weight nitrogen fraction when a specific inhibitor was added. Addition of chymostatin (0.01 mM) to sugar-salted herring gave 100% inhibition...

  20. Energetic Cost of Ichthyophonus Infection in Juvenile Pacific Herring (Clupea pallasii

    Directory of Open Access Journals (Sweden)

    Johanna J. Vollenweider

    2011-01-01

    Full Text Available The energetic costs of fasting and Ichthyophonus infection were measured in juvenile Pacific herring (Clupea pallasii in a lab setting at three temperatures. Infected herring incurred significant energetic costs, the magnitude of which depended on fish condition at the time of infection (fat versus lean. Herring that were fed continually and were in relatively good condition at the time of infection (fat never stored lipid despite ad libitum feeding. In feeding herring, the energetic cost of infection was a 30% reduction in total energy content relative to controls 52 days post infection. Following food deprivation (lean condition, infection caused an initial delay in the compensatory response of herring. Thirty-one days after re-feeding, the energetic cost of infection in previously-fasted fish was a 32% reduction in total energy content relative to controls. Body composition of infected herring subsequently recovered to some degree, though infected herring never attained the same energy content as their continuously fed counterparts. Fifty-two days after re-feeding, the energetic cost of infection in previously-fasted fish was a 6% reduction in total energy content relative to controls. The greatest impacts of infection occurred in colder temperatures, suggesting Ichthyophonus-induced reductions in body condition may have greater consequences in the northern extent of herring's range, where juveniles use most of their energy reserves to survive their first winter.

  1. Energetic Cost of Ichthyophonus Infection in Juvenile Pacific Herring (Clupea pallasii).

    Science.gov (United States)

    Vollenweider, Johanna J; Gregg, Jake L; Heintz, Ron A; Hershberger, Paul K

    2011-01-01

    The energetic costs of fasting and Ichthyophonus infection were measured in juvenile Pacific herring (Clupea pallasii) in a lab setting at three temperatures. Infected herring incurred significant energetic costs, the magnitude of which depended on fish condition at the time of infection (fat versus lean). Herring that were fed continually and were in relatively good condition at the time of infection (fat) never stored lipid despite ad libitum feeding. In feeding herring, the energetic cost of infection was a 30% reduction in total energy content relative to controls 52 days post infection. Following food deprivation (lean condition), infection caused an initial delay in the compensatory response of herring. Thirty-one days after re-feeding, the energetic cost of infection in previously-fasted fish was a 32% reduction in total energy content relative to controls. Body composition of infected herring subsequently recovered to some degree, though infected herring never attained the same energy content as their continuously fed counterparts. Fifty-two days after re-feeding, the energetic cost of infection in previously-fasted fish was a 6% reduction in total energy content relative to controls. The greatest impacts of infection occurred in colder temperatures, suggesting Ichthyophonus-induced reductions in body condition may have greater consequences in the northern extent of herring's range, where juveniles use most of their energy reserves to survive their first winter.

  2. Effects of scallop shell extract on scopolamine-induced memory impairment and MK801-induced locomotor activity

    OpenAIRE

    HASEGAWA, Yasushi; INOUE, Tatsuro; KAWAMINAMI, Satoshi; FUJITA, Miho

    2016-01-01

    ObjectiveTo evaluate the neuroprotective effects of the organic components of scallop shells (scallop shell extract) on memory impairment and locomotor activity induced by scopolamine or 5-methyl-10,11-dihydro-5H-dibenzo (a,d) cyclohepten-5,10-imine (MK801).MethodsEffect of the scallop shell extract on memory impairment and locomotor activity was investigated using the Y-maze test, the Morris water maze test, and the open field test.ResultsScallop shell extract significantly reduced scopolami...

  3. Pathogenecity of Ichthyophonus hoferi for laboratory-reared Pacific herring (Clupea pallasi) and its early appearance in wild Puget Sound herring

    Science.gov (United States)

    Kocan, R.; Hershberger, P.; Mehl, T.; Elder, N.; Bradley, M.; Wildermuth, D.; Stick, K.

    1999-01-01

    Laboratory-reared pathogen-free Pacific herring were exposed to pure cultures of Ichthyophonus hoferi, and reproduced the disease seen in naturally infected fish--thus fulfilling Koch's Postulates. Pathogen-free herring used in this study were reared from artificially spawned eggs incubated in filtered, UV-sterilized seawater, eliminating the variables associated with multiple infections, which are common in wild herring. Wild free-ranging herring were captured monthly from June through October by dip net from 'herring balls' located in the northern Puget Sound. I. hoferi infections were identified in these fish soon after metamorphoses, about 4 mo post-hatch. The prevalence increased from 5 to 6% in 0-yr fish to 24% in 1-yr-old fish to 50 to 70% in fish over 2 yr old, with no associated increase in mortality. The route of natural transmission to wild herring was not determined, but carnivorous fish became infected and died when they were experimentally fed tissues infected with the organism. In vitro culture of tissues was the most sensitive method for identifying both clinical and subclinical infections.

  4. Pathogenicity of Ichthyophonus hoferi for laboratory-reared Pacific herring Clupea pallasi and its early appearance in wild Puget Sound herring.

    Science.gov (United States)

    Kocan, R M; Hershberger, P; Mehl, T; Elder, N; Bradley, M; Wildermuth, D; Stick, K

    1999-01-07

    Laboratory-reared pathogen-free Pacific herring were exposed to pure cultures of Ichthyophonus hoferi, and reproduced the disease seen in naturally infected fish--thus fulfilling Koch's Postulates. Pathogen-free herring used in this study were reared from artificially spawned eggs incubated in filtered, UV-sterilized seawater, eliminating the variables associated with multiple infections, which are common in wild herring. Wild free-ranging herring were captured monthly from June through October by dip net from 'herring balls' located in the northern Puget Sound. I. hoferi infections were identified in these fish soon after metamorphoses, about 4 mo post-hatch. The prevalence increased from 5 to 6% in 0-yr fish to 24% in 1-yr-old fish to 50 to 70% in fish over 2 yr old, with no associated increase in mortality. The route of natural transmission to wild herring was not determined, but carnivorous fish became infected and died when they were experimentally fed tissues infected with the organism. In vitro culture of tissues was the most sensitive method for identifying both clinical and subclinical infections.

  5. Pathogenicity of Ichthyophonus hoferi for laboratory-reared Pacific herring Clupea pallasi and its early appearance in wild Puget Sound herring

    Science.gov (United States)

    Kocan, R.M.; Hershberger, P.; Mehl, T.; Elder, N.; Bradley, M.; Wildermuth, D.; Stick, K.

    1999-01-01

    Laboratory-reared pathogen-free Pacific herring were exposed to pure cultures of Ichthyophonus hoferi, and reproduced the disease seen in naturally infected fish - thus fulfilling Koch's Postulates. Pathogen-free herring used in this study were reared from artificially spawned eggs incubated in filtered, UV-sterilized seawater, eliminating the variables associated with multiple infections, which are common in wild herring. Wild free-ranging herring were captured monthly from June through October by dip net from 'herring balls' located in the northern Puget Sound. I. hoferi infections were identified in these fish soon after metamorphoses, about 4 mo post-hatch. The prevalence increased from 5 to 6% in 0-yr fish to 24% in 1-yr-old fish to 50 to 70% in fish over 2 yr old, with no associated increase in mortality. The route of natural transmission to wild herring was not determined, but carnivorous fish became infected and died when they were experimentally fed tissues infected with the organism. In vitro culture of tissues was the most sensitive method for identifying both clinical and subclinical infections.

  6. Structure of the New England herring gull population

    Science.gov (United States)

    Kadlec, J.A.; Drury, W.H.

    1968-01-01

    Measurements of the rates of population increase, reproduction, and mortality together with an observed age ratio, were used to analyze the population of the Herring Gull in New England. Data from sporadic censuses prior to this study, aerial censuses by the authors, and National Audubon Society Christmas Bird Count indicated that the New England breeding population has been doubling every 12 to 15 years since the early 1900's. This increase has involved founding new colonies and expanding the breeding range There is evidence that 15 to 30% of the adults do not breed in any given year. Sixty-one productivity measurements on 43 islands from 1963 through 1966, involving almost 13,000 nests, showed that from 0.8 to 1.4 young/breeding pair/year is the usual range of rate of production. The age distribution in the population was determined by classifying Herring Gulls by plumage category on an aerial census of the coast from Tampico, Mexico, to Cape Sable, Nova Scotia. Of the 622,000 gulls observed, 68% were adults, 17% were second- and third-year birds, and 15% were first-year birds. Mortality rates derived from band recovery data were too high to be consistent with the observed rate of population growth, productivity, and age structure. Loss of bands increasing to the rate of about 20%/year 5 years after banding eliminates most of the discrepancy. The age structure and rate of population increase indicate a mortality rate of 4 to 9% for gulls 2 years old or older, compared with the 25 to 30% indicated by band recoveries. The population structure we have developed fits everything we have observed about Herring Gull population dynamics, except mortality based on band recoveries.

  7. Multidecadal, centennial, and millennial variability in sardine and anchovy abundances in the western North Pacific and climate-fish linkages during the late Holocene

    Science.gov (United States)

    Kuwae, Michinobu; Yamamoto, Masanobu; Sagawa, Takuya; Ikehara, Ken; Irino, Tomohisa; Takemura, Keiji; Takeoka, Hidetaka; Sugimoto, Takashige

    2017-12-01

    Paleorecords of pelagic fish abundance could better define the nature of fishery productivity dynamics and help understand responses of pelagic fish stocks to long-term climate changes. We report a high-resolution record of sardine and anchovy scale deposition rates (SDRs) from Beppu Bay, Southwest Japan, showing multidecadal and centennial variability in the abundance of Japanese sardine and Japanese anchovy during the last 2850 years. Variations in the sardine SDR showed periodicities at ∼50, ∼100, and ∼300 yr, while variations in the anchovy SDR showed periodicities at ∼30 and ∼260 yr. Comparisons between and correlation analyses of the time series of the sardine and anchovy SDRs demonstrate that there is not a consistent out-of-phase relationship during the last 2850 years. This indicates that the multidecadal alternations in the sardine and anchovy populations commonly seen in the 20th century did not necessarily occur during earlier periods. The Japanese sardine SDR record shows a long-term decreasing trend in the amplitudes of the multidecadal to centennial fluctuations. This decreasing trend may have resulted from an increasing trend in the winter sea surface temperature in the western North Pacific. The multicentennial variability in sardine abundance during the last millennium is consistent with the variabilities in the abnormal snow index in East Asia and the American tree ring-based Pacific Decadal Oscillation index, suggesting a basin-wide or regional climate-marine ecosystem linkage.

  8. Effect of the Gamma Radiation and Temperature on Histamine Production, Lipid Peroxidation and Antioxidant Parameters in Sardine (Sardina Pilchardus)

    International Nuclear Information System (INIS)

    Maltar-Strmecki, N.; Aladrovic, J.; Dzaja, P.; Ljubic-Beer, B.; Laskaj, R.

    2013-01-01

    Radiation processing of fish is recognized as a safe and effective method for reducing microorganisms and viruses as well for inactivating pathogens among the existing technologies for preservation. Safety and hygienic quality is directly related to the duration between when the fish is caught and when it reaches the end consumer and depends upon conditions how the sardine is handled and upon which conditions. As sardine (Sardina pilchardus Walbaum, 1792) is pelagric fish widely distributed in the Adriatic Sea and one of the most commercially important fish species in the fisheries of all countries located along the coast of the Adriatic Sea in the present study, the effects of gamma irradiation on the histamine production, lipid peroxidation and antioxidant parameters in sardine during the storage at two different temperatures (4 and 30 degrees of Celsius) were investigated. The results indicate that histamine concentration was reduced by gamma irradiation and that the safe consumption can be prolonged for both temperatures of storage. However, irradiation treatment induced oxidative damage, as evidenced by changes in levels of lipid peroxidation and radical kinetic rate detected by EPR (electron paramagnetic resonance) spectroscopy. These results suggest that gamma radiation undoubtedly induces antioxidant defence system in sardine fish. However, further research is necessary to elucidate the precise role that the antioxidant system plays under the influence of gamma radiation and temperature.(author)

  9. Dietary sardine protein lowers insulin resistance, leptin and TNF-α and beneficially affects adipose tissue oxidative stress in rats with fructose-induced metabolic syndrome.

    Science.gov (United States)

    Madani, Zohra; Louchami, Karim; Sener, Abdullah; Malaisse, Willy J; Ait Yahia, Dalila

    2012-02-01

    The present study aims at exploring the effects of sardine protein on insulin resistance, plasma lipid profile, as well as oxidative and inflammatory status in rats with fructose-induced metabolic syndrome. Rats were fed sardine protein (S) or casein (C) diets supplemented or not with high-fructose (HF) for 2 months. Rats fed the HF diets had greater body weight and adiposity and lower food intake as compared to control rats. Increased plasma glucose, insulin, HbA1C, triacylglycerols, free fatty acids and impaired glucose tolerance and insulin resistance was observed in HF-fed rats. Moreover, a decline in adipose tissues antioxidant status and a rise in lipid peroxidation and plasma TNF-α and fibrinogen were noted. Rats fed sardine protein diets exhibited lower food intake and fat mass than those fed casein diets. Sardine protein diets diminished plasma insulin and insulin resistance. Plasma triacylglycerol and free fatty acids were also lower, while those of α-tocopherol, taurine and calcium were enhanced as compared to casein diets. Moreover, S-HF diet significantly decreased plasma glucose and HbA1C. Sardine protein consumption lowered hydroperoxide levels in perirenal and brown adipose tissues. The S-HF diet, as compared to C-HF diet decreased epididymal hydroperoxides. Feeding sardine protein diets decreased brown adipose tissue carbonyls and increased glutathione peroxidase activity. Perirenal and epididymal superoxide dismutase and catalase activities and brown catalase activity were significantly greater in S-HF group than in C-HF group. Sardine protein diets also prevented hyperleptinemia and reduced inflammatory status in comparison with rats fed casein diets. Taken together, these results support the beneficial effect of sardine protein in fructose-induced metabolic syndrome on such variables as hyperglycemia, insulin resistance, hyperlipidemia and oxidative and inflammatory status, suggesting the possible use of sardine protein as a protective

  10. Effect of different types of coatings on texture and nutritional properties of canned Portuguese sardines (Sardina pilchardus

    Directory of Open Access Journals (Sweden)

    Rita Pinheiro

    2014-06-01

    Full Text Available Canned foods have a long history of success and are likely to remain popular for the foreseeable future owing to their convenience, long shelf life and economy. Interest in fish consumption has increased in recent years due to the wide range of its health benefits. In addition to fresh product, canned fish enables a delayed consumption of this appreciated kind of food. Among the different types of fish, in Portugal, the most used in canning manufacture are sardines, tuna, anchovies, mackerels. Vegetable oils and tomato sauce are usually adopted as coatings (liquid medium. In fact, oil has a preserving effect and contributes to make the product more palatable. Its protective action lies in the ability to insulate products from air, rather than having an active bacteriostatic or bactericidal action. Among the different types of coatings the most commonly used in canning are: olive oil, seed oils and different sauces, such as tomato sauce. Tomato sauce has also vegetable oil on its formulation. The amount of coating substance can affect the nutritional composition as well as texture properties of the sardines. The aim of this work was to investigate the effect of different types of coatings on texture and nutritional properties of whole, skinless and boneless canned Portuguese Sardines (Sardina pilchardus. An experimental investigation was carried out to assess the protein, lipid, carbohydrates and ash content, texture and colour profile of canned sardine with different kinds of coatings: olive oil, sunflower oil and tomato sauce. The obtained results showed that protein and carbohydrates content were not significantly influenced by the coating used, varying between 5%-8% and13%-18%, respectively. For the moisture content it was found that the samples with tomato sauce were 2.2-fold higher than the samples with sunflower oil, and 1.3-fold higher than the samples with olive oil, independently of being whole, skinless or boneless sardine. As

  11. Empirically based models of oceanographic and biological influences on Pacific Herring recruitment in Prince William Sound

    Science.gov (United States)

    Sewall, Fletcher; Norcross, Brenda; Mueter, Franz; Heintz, Ron

    2018-01-01

    Abundances of small pelagic fish can change dramatically over time and are difficult to forecast, partially due to variable numbers of fish that annually mature and recruit to the spawning population. Recruitment strength of age-3 Pacific Herring (Clupea pallasii) in Prince William Sound, Alaska, is estimated in an age-structured model framework as a function of spawning stock biomass via a Ricker stock-recruitment model, and forecasted using the 10-year median recruitment estimates. However, stock size has little influence on subsequent numbers of recruits. This study evaluated the usefulness of herring recruitment models that incorporate oceanographic and biological variables. Results indicated herring recruitment estimates were significantly improved by modifying the standard Ricker model to include an index of young-of-the-year (YOY) Walleye Pollock (Gadus chalcogrammus) abundance. The positive relationship between herring recruits-per-spawner and YOY pollock abundance has persisted through three decades, including the herring stock crash of the early 1990s. Including sea surface temperature, primary productivity, and additional predator or competitor abundances singly or in combination did not improve model performance. We suggest that synchrony of juvenile herring and pollock survival may be caused by increased abundance of their zooplankton prey, or high juvenile pollock abundance may promote prey switching and satiation of predators. Regardless of the mechanism, the relationship has practical application to herring recruitment forecasting, and serves as an example of incorporating ecosystem components into a stock assessment model.

  12. Infecting Pacific Herring with Ichthyophonus sp. in the laboratory

    Science.gov (United States)

    Hershberger, Paul; Hart, Lucas; Mackenzie, Ashley; Yanney, M.L.; Conway, Carla M.; Elliott, Diane G.

    2015-01-01

    The protistan parasite Ichthyophonus sp. occurs in coastal populations of Pacific Herring Clupea pallasii throughout the northeast Pacific region, but the route(s) by which these planktivorous fish become infected is unknown. Several methods for establishing Ichthyophonus infections in laboratory challenges were examined. Infections were most effectively established after intraperitoneal (IP) injections with suspended parasite isolates from culture or after repeated feedings with infected fish tissues. Among groups that were offered the infected tissues, infection prevalence was greater after multiple feedings (65%) than after a single feeding (5%). Additionally, among groups that were exposed to parasite suspensions prepared from culture isolates, infection prevalence was greater after exposure by IP injection (74%) than after exposure via gastric intubation (12%); the flushing of parasite suspensions over the gills did not lead to infections in any of the experimental fish. Although the consumption of infected fish tissues is unlikely to be the primary route of Ichthyophonus sp. transmission in wild populations of Pacific Herring, this route may contribute to abnormally high infection prevalence in areas where juveniles have access to infected offal.

  13. Infecting Pacific Herring with Ichthyophonus sp. in the Laboratory.

    Science.gov (United States)

    Hershberger, P K; Hart, L M; MacKenzie, A H; Yanney, M L; Conway, C M; Elliott, D G

    2015-12-01

    The protistan parasite Ichthyophonus sp. occurs in coastal populations of Pacific Herring Clupea pallasii throughout the northeast Pacific region, but the route(s) by which these planktivorous fish become infected is unknown. Several methods for establishing Ichthyophonus infections in laboratory challenges were examined. Infections were most effectively established after intraperitoneal (IP) injections with suspended parasite isolates from culture or after repeated feedings with infected fish tissues. Among groups that were offered the infected tissues, infection prevalence was greater after multiple feedings (65%) than after a single feeding (5%). Additionally, among groups that were exposed to parasite suspensions prepared from culture isolates, infection prevalence was greater after exposure by IP injection (74%) than after exposure via gastric intubation (12%); the flushing of parasite suspensions over the gills did not lead to infections in any of the experimental fish. Although the consumption of infected fish tissues is unlikely to be the primary route of Ichthyophonus sp. transmission in wild populations of Pacific Herring, this route may contribute to abnormally high infection prevalence in areas where juveniles have access to infected offal.

  14. Baltic Herring Fisheries Management: Stakeholder Views to Frame the Problem

    Directory of Open Access Journals (Sweden)

    Päivi Haapasaari

    2012-09-01

    Full Text Available Comprehensive problem framing that includes different perspectives is essential for holistic understanding of complex problems and as the first step in building models. We involved five stakeholders to frame the management problem of the Central Baltic herring fishery. By using the Bayesian belief networks (BBNs approach, the views of the stakeholders were built into graphical influence diagrams representing variables and their dependencies. The views of the scientists involved concentrated on biological concerns, whereas the fisher, the manager, and the representative of an environmental nongovernmental organization included markets and fishing industry influences. Management measures were considered to have a relatively small impact on the development of the herring stock; their impact on socioeconomic objectives was greater. Overall, the framings by these stakeholders propose a focus on socioeconomic issues in research and management and explicitly define management objectives, not only in biological but also in social and economic terms. We find the approach an illustrative tool to structure complex issues systematically. Such a tool can be used as a forum for discussion and for decision support that explicitly includes the views of different stakeholder groups. It enables the examination of social and biological factors in one framework and facilitates bridging the gap between social and natural sciences. A benefit of the BBN approach is that the graphical model structures can be transformed into a quantitative form by inserting probabilistic information.

  15. Effects of scallop shell extract on scopolamine-induced memory impairment and MK801-induced locomotor activity.

    Science.gov (United States)

    Hasegawa, Yasushi; Inoue, Tatsuro; Kawaminami, Satoshi; Fujita, Miho

    2016-07-01

    To evaluate the neuroprotective effects of the organic components of scallop shells (scallop shell extract) on memory impairment and locomotor activity induced by scopolamine or 5-methyl-10,11-dihydro-5H-dibenzo (a,d) cyclohepten-5,10-imine (MK801). Effect of the scallop shell extract on memory impairment and locomotor activity was investigated using the Y-maze test, the Morris water maze test, and the open field test. Scallop shell extract significantly reduced scopolamine-induced short-term memory impairment and partially reduced scopolamine-induced spatial memory impairment in the Morris water maze test. Scallop shell extract suppressed scopolamine-induced elevation of acetylcholine esterase activity in the cerebral cortex. Treatment with scallop shell extract reversed the increase in locomotor activity induced by scopolamine. Scallop shell extract also suppressed the increase in locomotor activity induced by MK801. Our results provide initial evidence that scallop shell extract reduces scopolamine-induced memory impairment and suppresses MK-801-induced hyperlocomotion. Copyright © 2016 Hainan Medical College. Production and hosting by Elsevier B.V. All rights reserved.

  16. Scalloped Implant-Abutment Connection Compared to Conventional Flat Implant-Abutment Connection

    DEFF Research Database (Denmark)

    Starch-Jensen, Thomas; Christensen, Ann-Eva; Lorenzen, Henning

    2017-01-01

    OBJECTIVES: The objective was to test the hypothesis of no difference in implant treatment outcome after installation of implants with a scalloped implant-abutment connection compared to a flat implant-abutment connection. MATERIAL AND METHODS: A MEDLINE (PubMed), Embase and Cochrane library search......-abutment connection. There were no significant differences between the two treatment modalities regarding professional or patient-reported outcome measures. Meta-analysis disclosed a mean difference of peri-implant marginal bone loss of 1.56 mm (confidence interval: 0.87 to 2.25), indicating significant more bone...... loss around implants with a scalloped implant-abutment connection. CONCLUSIONS: A scalloped implant-abutment connection seems to be associated with higher peri-implant marginal bone loss compared to a flat implant-abutment connection. Therefore, the hypothesis of the present systematic review must...

  17. Temperature and food-mediated variability of European Atlantic sardine recruitment

    Science.gov (United States)

    Garrido, Susana; Silva, Alexandra; Marques, Vitor; Figueiredo, Ivone; Bryère, Philippe; Mangin, Antoine; Santos, A. Miguel P.

    2017-12-01

    The influence of the environmental conditions during larval development on the resulting recruitment strength was investigated for European sardine (Sardina pilchardus) at Atlanto-Iberian waters. Satellite-derived Sea Surface Temperature (SST) and Chlorophyll-a concentration (Chla) data from the previous spawning seasons (January to March/April and October to December of the previous year) were related to recruitment success data in the main recruitment hotspots. Recruitment data was taken from yearly acoustic scientific cruises and from the ICES recruitment index estimated by an age-structured model for the entire stock. A linear discriminant analysis model using SST, Chla, and the abundance of spawners during the spawning season identified years of high and low recruitment for all the recruitment hotspots with an accuracy of ≥79%. In general, high recruitment years were associated with high Chla and low SST, although the most important variables to discriminate between the groups were area-specific. High recruitment years were mostly related to high food availability (Chla), particularly during the last quarter of the previous year. In Western Iberia and in the Gulf of Cadiz, high recruitment years were also associated to lower SST, whereas in the Bay of Biscay, where SST during the winter was generally below the optimal range ≈11-12 °C for sardine larval development, higher recruitment was associated with high SST. For ICES data of the southern European sardine stock, lower SST and higher Chla during the last quarter of the previous year were associated with high recruitment years and SST alone was able to discriminate between the two recruitment groups with 73% accuracy. Although the time-series of available data are still small, these significant relationships are consistent with field and laboratory studies relating larval growth and mortality with main environmental drivers. These relationships should be further investigated in the following years to

  18. Tracing the origin of paralytic shellfish toxins in scallop Patinopecten yessoensis in the northern Yellow Sea.

    Science.gov (United States)

    Chen, Jian-Hua; Yu, Ren-Cheng; Gao, Yan; Kong, Fan-Zhou; Wang, Yun-Feng; Zhang, Qing-Chun; Kang, Zhen-Jun; Yan, Tian; Zhou, Ming-Jiang

    2013-01-01

    Some dinoflagellate species within the genera Alexandrium, Gymnodinium and Pyrodinium are well-known producers of paralytic shellfish toxins (PST), which led to many poisoning incidents around the world. In the northern Yellow Sea, an important mariculture zone for scallop Patinopecten yessoensis, PST have been frequently detected from scallops. However, there is little knowledge concerning PST-producing microalgae in this region so far. In cruises carried out in 2011 and 2012, scallop and phytoplankton samples were collected from the northern Yellow Sea. PST were detected from scallops by high-performance liquid chromatography with fluorescence detection (HPLC-FLD). Toxin content and profile were remarkably different among the four tissues, i.e. viscera, adductor muscle, mantle and gonad, suggesting apparent toxin transfer and transformation in scallops. Viscera always had the highest content of PST dominated by low-potency N-sulfocarbamoyl toxins C1 and C2, which closely resembled the toxin profiles of net-concentrated phytoplankton samples in spring. Based on the morphological features, cells of Alexandrium spp. in net-concentrated phytoplankton samples were picked out and a partial sequence of the large subunit ribosomal RNA gene (LSU rDNA) was amplified using a single-cell polymerase chain reaction (PCR) method. Cells of both toxic A. tamarense species complex and non-toxic A. affine were identified from the phytoplankton samples based on the partial LSU rDNA sequence information. According to these findings, it is implied that A. tamarense species complex is the major toxic species related to PST contamination in scallops of the northern Yellow Sea. The presence of both toxic and non-toxic Alexandrium spp. in this region requires for a species-specific method to monitor the distribution and dynamics of A. tamarense species complex.

  19. Immunotoxicity effect of benzo[α]pyrene on scallop Chlamys farreri

    Science.gov (United States)

    Zhang, Lin; Pan, Luqing; Liu, Jing

    2009-03-01

    The toxic effects of benzo[ α]pyrene (B[ α]P) at different concentrations (0.1, 0.5, 1, 2.5 and 7.5 μgL-1) on scallop ( Chlamy farreri) immune system were studied. The results showed that B[ α]P had significant toxic effects on the haemocyte counts, neutral red uptake, phagocytosis, bacteriolytic and antibacterial activity ( P0.05). Thus, B[ α] has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution and immunomodulation in aquatic organisms.

  20. sardine run

    African Journals Online (AJOL)

    1989-07-17

    Jul 17, 1989 ... Marine mammals are long- lived, have large lipid reserves in proportion to their body size and mass and are therefore ideal repositories for high concentrations of these pollutants. Also, be- cause they apparently lack certain enzymes for the meta- bolism of organochlorines, they may accumulate these.

  1. Arsenic, cadmium, lead and mercury in canned sardines commercially available in eastern Kentucky, USA

    International Nuclear Information System (INIS)

    Shiber, John G.

    2011-01-01

    Research highlights: → Total As, Cd, Pb and Hg in canned sardines within ranges of other studies. → As highest in samples from Norway (1.87 μg/g) and Thailand (1.63 μg/g). → Cd highest in Moroccan (0.07 μg/g), Pb in Canadian (0.27 μg/g); Hg not detected. → Lack of established limits for As and Cd in fish restricts interpretation of results. → Rise of small pelagics in human diet warrants more scrutiny on their metal content. - Abstract: Seventeen samples of canned sardines, originating from six countries and sold in eastern Kentucky, USA, were analyzed in composites of 3-4 fish each for total arsenic (As), cadmium (Cd) and lead (Pb) by graphite furnace atomic absorption spectrophotometry (AAS) and for mercury (Hg) by thermal decomposition amalgamation and AAS. Results in μg/g wet: As 0.49-1.87 (mean: 1.06), Cd < 0.01-0.07 (0.03), Pb < 0.06-0.27 (0.11), Hg ND < 0.09. Values fall generally within readings reported by others, but no internationally agreed upon guidelines have yet been set for As or Cd in canned or fresh fish. The incidence of cancers and cardiovascular diseases associated with As ingestion is extraordinarily high here. With the role of food-borne As in human illness presently under scrutiny and its maximum allowable limits in fish being reviewed, more studies of this nature are recommended, especially considering the potential importance of small pelagic fishes as future seafood of choice.

  2. The Gordian knot: managing herring (Clupea harengus) bridging across populations, fishery units, management areas, and politics

    DEFF Research Database (Denmark)

    Worsøe Clausen, Lotte; Mosegaard, Henrik; Berg, Casper Willestofte

    2012-01-01

    –24) and the North Sea (Division IIIa) by various EU—and in the latter case also non‐EU—fishing fleets. For the two separate management areas, TACs are set at different times in the yearly TACsetting process by the EU and negotiating counties, which often result in conflicts over quota allocations among different......The management of western Baltic spring spawning (WBSS) herring is challenged by the highly complex stock structure with a temporal and geographical distribution leading to conflicting interests among different stakeholder groups. The stock is exploited in the Baltic Sea (Subdivisions 22...... management units. The WBSS herring stock spawns in the western Baltic Sea and migrates into the Kattegat and Skagerrak areas, where it mixes with North Sea autumn spawning (NSAS) herring. Recent development of otolith shape analysis has enabled a high‐resolution separation of herring stocks in these waters...

  3. Recurrent mass mortalities of the blueback herring, Alosa aestivalis, in the lower Connecticut River

    International Nuclear Information System (INIS)

    Moss, S.A.; Leggett, W.C.; Boyd, W.A.

    1976-01-01

    The authors studied 5 mass mortalities of blueback herring, Alosa aestivalis between 1965 and 1971. The reach of the Connecticut River in which the mortalities were observed includes the region thermally influenced by cooling water effluents from the Connecticut Yankee Atomic Power Plant. This warm water effluent has not been related to catastrophic blueback herring mortalities to the present time. The mortalities observed in 1965, 1966, and 1967 occurred before the plant became operational. The years of no abnormal mortalities, 1968, 1969, and 1970 coincided with periods of near-maximum generating output by this power plant. Only in June, 1971, did power plant operation coincide with a mass mortality of herring. Nevertheless, the occurrence of herring mortalities in four years out of seven observed suggests that further deterioration of water quality in the Connecticut River may threaten the continued spawning success of this fish. 9 references, 4 figures, 4 tables

  4. AFSC/ABL: Southeast and Prince William Sound, Alaska Herring Microsatellite data, 2007-2008

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Herring is one of the most energy-rich fish in the Alaskan ecosystem, and when populations struggle over time, such as the Lynn Canal population, there is management...

  5. Chasing red herrings: Memory of distractors causes fixation in creative problem solving.

    Science.gov (United States)

    Beda, Zsolt; Smith, Steven M

    2018-03-07

    Two experiments tested the red herring retrieval hypothesis, which states that fixation in creative problem solving is worse when memory for red herrings (i.e., inappropriate or incorrect solutions) is strengthened. In Experiment 1, when associations between Remote Associates Test (RAT) problem words (e.g., COTTAGE, SWISS, CAKE) and related red herring words (e.g., hut, chocolate, icing) were strengthened via repetition, an encoding variable, fixation was found to increase. In Experiment 2, when associations were formed between RAT problem contexts and red herrings, then subsequent reinstatement of problem contexts during RAT problem solving trials (as compared with showing new contexts) also worsened fixation effects. Our results add to the increasing body of research that connects memory with creative problem solving, and they suggest possible ways for overcoming fixation effects.

  6. Addressing socio-cultural values in the use and management of Baltic herring

    DEFF Research Database (Denmark)

    Ignatius, Suvi; Haapasaari, Päivi Elisabet

    2016-01-01

    , and is mainly used for animal feed and fishmeal and oil. Harmful dioxins in Baltic herring have been offered as an explanation for its reduced human consumption. We argue that dioxins are only part of the explanation while sociocultural values also influence the use and management of the fish resource....... By applying the justification theory of Boltanski and Thévenot to expert workshop discussions and a literature review, we investigate (1) what values people in Sweden, Finland, Denmark and Estonia associate with Baltic herring; and (2) which values determine the way the fish resource is used. Our study...... indicates that management decisions related to Baltic herring are predominantly guided by economic values and industrial efficiency. Profit optimisation in the fishery together with the image problem that Baltic herring suffers from, seem to have contributed to the devaluation of this fish species as food...

  7. Properties of protein powders from arrowtooth flounder (Atheresthes stomias) and herring (Clupea harengus) byproducts.

    Science.gov (United States)

    Sathivel, Subramaniam; Bechtel, Peter J; Babbitt, Jerry; Prinyawiwatkul, Witoon; Negulescu, Ioan I; Reppond, Kermit D

    2004-08-11

    Functional, nutritional, and thermal properties of freeze-dried protein powders (FPP) from whole herring (WHP), herring body (HBP), herring head (HHP), herring gonad (HGP), and arrowtooth flounder fillets (AFP) were evaluated. The FPP samples have desirable nutritional and functional properties and contained 63-81.4% protein. All FPP samples had desirable essential amino acid profiles and mineral contents. The emulsifying and fat adsorption capacities of all FPP samples were higher than those of soy protein concentrate. The emulsifying stability of WHP was lower than that of egg albumin but greater than that of soy protein concentrate. Thermal stability of the FPP samples is in the following order: HGP > HBP > WHP > HHP > AFP.

  8. LANDSAT menhaden and thread herring resources investigation. [Gulf of Mexico

    Science.gov (United States)

    Kemmerer, A. J. (Principal Investigator); Brucks, J. T.; Butler, J. A.; Faller, K. H.; Holley, H. J.; Leming, T. D.; Savastano, K. J.; Vanselous, T. M.

    1977-01-01

    The author has identified the following significant results. The relationship between the distribution of menhaden and selected oceanographic parameters (water color, turbidity, and possibly chlorophyll concentrations) was established. Similar relationships for thread herring were not established nor were relationships relating to the abundance of either species. Use of aircraft and LANDSAT remote sensing instruments to measure or infer a set of basic oceanographic parameters was evaluated. Parameters which could be accurately inferred included surface water temperature, salinity, and color. Water turbidity (Secchi disk) was evaluated as marginally inferrable from the LANDSAT MSS data and chlorophyll-a concentrations as less than marginal. These evaluations considered the parameters only as experienced in the two test areas using available sensors and statistical techniques.

  9. Incorporating stakeholders' knowledge to stock assessment: Central Baltic herring

    DEFF Research Database (Denmark)

    Mäntyniemi, Samu; Haapasaari, Päivi Elisabet; Kuikka, Sakari

    2013-01-01

    We present a method by which the knowledge of stakeholders can be taken into account in stock assessment. The approach consists of a structured interview process followed by quantitative modelling of the answers. The outcome is a set of probability models, each describing the views of different...... stakeholders. Individual models are then merged to a large model by applying the techniques of Bayesian model averaging, and this model is conditioned on stock assessment data. As a result, the viewsofinterviewedstakeholdershavebeentakenintoaccountandweighedbasedonhowwelltheirviewsaresupportedbythe observed...... data. We applied this method to the Baltic Sea herring (Clupea harengus) stock assessment by interviewing six stakeholders and conditioning the resulting models on stock assessment data provided by the International Council for the Exploration of the Sea....

  10. Organohalogen pollutants in herring from the northern Baltic Sea: Concentrations, congener profiles and explanatory factors

    International Nuclear Information System (INIS)

    Koistinen, Jaana; Kiviranta, Hannu; Ruokojaervi, Paeivi; Parmanne, Raimo; Verta, Matti; Hallikainen, Anja; Vartiainen, Terttu

    2008-01-01

    Organohalogen contaminants were investigated in Baltic herring caught from three catchment areas in the Baltic Sea, off the coasts of Finland. Pools of both small and large herring were analysed for polychlorinated biphenyls (PCB), dibenzo-p-dioxins, dibenzofurans, naphthalenes, camphenes (toxaphene), polybrominated diphenyl ethers and the pesticide DDT and its metabolites. PCB concentrations per fresh weight in small herring were at the same level in all catchment areas, i.e. the Bothnian Bay, the Bothnian Sea and the Gulf of Finland, revealing no hot spots and reflecting most likely long term emissions and atmospheric deposition. Differences in the levels and/or congener profiles of other contaminants between catchment areas may be explained by point sources. Similar concentrations in small and large herring in the Gulf of Finland were possibly due to their common nutrition. In the other areas, differences between small and large herring most likely reflected their different food sources. - Sources of pollutants are reflected in levels and congener profiles of pollutants in Baltic herring

  11. The Adopt-a-Herring program as a fisheries conservation tool

    Science.gov (United States)

    Frank, Holly J.; Mather, Martha E.; Muth, Robert M.; Pautzke, Sarah M.; Smith, Joseph M.; Finn, John T.

    2009-01-01

    Successful conservation depends on a scientifically literate public. We developed the adopt-a-Herring program to educate nonscientists about fisheries and watershed restoration. this interactive educational and outreach project encouraged coastal residents to be involved in local watershed restoration. In the northeastern United States, river herring (Alosa spp.) are an important component of many coastal watersheds and often are the object of conservation efforts. In order to understand river herring spawning behavior and to improve the effectiveness of restoration efforts, our research tracked these fish via radiotelemetry in the Ipswich River, Massachusetts. In our adopt-a-Herring Program, participating stakeholder organizations adopted and named individual tagged river herring and followed their movements online. We also made information available to our adopters on our larger research goals, the mission and activities of other research and management agencies, examples of human actions that adversely affect watersheds, and opportunities for proactive conservation. Research results were communicated to adopters through our project web page and end-of-the-season summary presentations. Both tools cultivated a personal interest in river herring, stimulated discussion about fisheries and watershed restoration, educated participants about the goals and methods of scientists in general, and initiated critical thinking about human activities that advance or impede sustainability.

  12. Data on color and chemical composition of dried scallop (Mizuhopecten yessoensis produced in different areas of Hokkaido, Japan

    Directory of Open Access Journals (Sweden)

    Hisaki Enda

    2018-02-01

    Full Text Available Dried scallop is used in Chinese, Japanese, and French cuisines for its unique flavor and taste. The quality of dried scallop is rated according to its clear, shiny brown color, developed by the Maillard reaction between sugars and amino acids. This article reports the colors, represented by L* and a* values, and chemical composition (water, salinity, Brix, proteins, and amino acids of dried scallop products. The dried scallops were produced in Tokoro, Sarufutsu, and Saroma in Hokkaido, Japan. The color of the dried scallops had values of 45.7–52.0 for L* and 2.31–5.08 for a*. The salinity of the products was 15.1–17.7%. The amino acid contents were 1350.8–1668.6 mg/100 g. The data collected here are provided in table format. The data can serve as a reference for commercial dried scallop products to determine product quality. Keywords: Quality of products, Dried scallop, Seasoning ingredient, Browning

  13. Effect of longitudinal grooves of the scallop surface on aerodynamic performance

    International Nuclear Information System (INIS)

    Kim, Tae Hun; Choi, Hae Cheon

    2008-01-01

    Some of the scallops like Amesium balloti have an excellent level-swimming ability, i.e. they can swim about 20m by single level swimming with a maximum swimming velocity of about 1.6m/s in the sea. On the other hand, some species like Patinopecten yessoensis have longitudinal grooves on the upper and lower surfaces and others do not. Therefore, in the present study, we measure the lift and drag forces on a real scallop model (Patinopecten yessoensis) in a wind tunnel. Experiments are performed at the Reynolds number of 75,000 based on the maximum chord length, which is within the swimming condition of real scallop (Re=30,000∼300,000). To see the effect of longitudinal grooves, we measure the aerodynamic forces on a scallop model by removing the grooves. With the grooves, the lift force increases at low angles of attack (α<10 .deg.). The drag force increases slightly at all the attack angles considered. The lift-to-drag ratio is increased by about 10% at α<10 .deg.

  14. 76 FR 23940 - Fisheries of the Northeastern United States; Atlantic Sea Scallop Fishery; Framework Adjustment 22

    Science.gov (United States)

    2011-04-29

    ... the LA fleet, Amendment 15 proposes a management uncertainty buffer based on the F associated with a... to implement Framework Adjustment 22 (Framework 22) to the Atlantic Sea Scallop Fishery Management Plan (FMP), which was developed and adopted by the New England Fishery Management Council (Council) and...

  15. Co-evolution of polygonal and scalloped terrains, southwestern Utopia Planitia, Mars

    Science.gov (United States)

    Haltigin, T. W.; Pollard, W. H.; Dutilleul, P.; Osinski, G. R.; Koponen, L.

    2014-02-01

    Thermal contraction crack polygons and scalloped depressions, two of the most common landforms found in Utopia Planitia, Mars, have previously been linked to the presence of ice-rich deposits in the subsurface. Although the formation and evolution of these features individually are relatively well understood, little to no effort has been directed towards elucidating possible interactions that occur between them during their development. Thus, the overarching goal of this research was to investigate if there is an evolutionary link between polygonal and scalloped terrains by correlating metrics representing polygon and scallop maturity. A variety of statistical analyses were performed using HiRISE and MOLA datasets to quantify interactions between four sets of polygonal and scalloped terrains. Our results demonstrate the existence of a negative relationship between polygonal subdivision and surface elevation, indicating that polygon networks become more ‘evolved’ as the surface subsides. These results suggest that the permafrost landscape in Utopia Planitia may once have been extremely ice-rich, and that multiple geomorphic processes may be responsible for its evolution. Ultimately, this work demonstrates that landscape reconstruction is more complete when a system approach is followed, quantifying interactions between landforms as opposed to examining an individual landform in isolation.

  16. FREEZE-THAW TEST RESULTS OF POROUS CONCRETE WITH CRUSHED SCALLOP SHELL MATERIAL ADDED

    OpenAIRE

    SUGIYAMA, Masashi

    2004-01-01

    This paper addresses the possibility of recycling an abundantly available byproduct of the fisheries industry, scallop shells, by adding it to porous concrete. If these shells, now discarded as refuse, can be used as an additive in porous concrete, this c

  17. The Scallop's Eye--A Concave Mirror in the Context of Biology

    Science.gov (United States)

    Colicchia, Giuseppe; Waltner, Christine; Hopf, Martin; Wiesner, Hartmut

    2009-01-01

    Teaching physics in the context of medicine or biology is a way to generate students' interest in physics. A more uncommon type of eye, the scallop's eye (an eye with a spherical concave mirror, which is similar to a Newtonian or Schmidt telescope) and the image-forming mechanism in this eye are described. Also, a simple eye model, which can…

  18. 75 FR 63721 - Fisheries of the Northeastern United States; Atlantic Sea Scallop Fishery; Framework Adjustment...

    Science.gov (United States)

    2010-10-18

    ... 2010 scallop fishing year (FY). Following publication, NMFS identified errors, omissions, and possible..., 2010, start of the fishing year (FY) and the implementation of Framework 21 FY 2010 management measures... comment specifically addressing the provision for Elephant Trunk Access Area (ETAA) trip overages for...

  19. A Dopa Decarboxylase Modulating the Immune Response of Scallop Chlamys farreri

    Science.gov (United States)

    Zhou, Zhi; Yang, Jialong; Wang, Lingling; Zhang, Huan; Gao, Yang; Shi, Xiaowei; Wang, Mengqiang; Kong, Pengfei; Qiu, Limei; Song, Linsheng

    2011-01-01

    Background Dopa decarboxylase (DDC) is a pyridoxal 5-phosphate (PLP)-dependent enzyme that catalyzes the decarboxylation of L-Dopa to dopamine, and involved in complex neuroendocrine-immune regulatory network. The function for DDC in the immunomodulation remains unclear in invertebrate. Methodology The full-length cDNA encoding DDC (designated CfDDC) was cloned from mollusc scallop Chlamys farreri. It contained an open reading frame encoding a polypeptide of 560 amino acids. The CfDDC mRNA transcripts could be detected in all the tested tissues, including the immune tissues haemocytes and hepatopancreas. After scallops were treated with LPS stimulation, the mRNA expression level of CfDDC in haemocytes increased significantly (5.5-fold, PDDC inhibitor methyldopa, the ROS level in haemocytes of scallops was decreased significantly to 0.41-fold (PDDC in scallop, modulated the immune responses such as haemocytes encapsulation as well as the ROS level through its catalytic activity, functioning as an indispensable immunomodulating enzyme in the neuroendocrine-immune regulatory network of mollusc. PMID:21533240

  20. Regional variation in the intensity of humpback whale predation on Pacific herring in the Gulf of Alaska

    Science.gov (United States)

    Moran, J. R.; Heintz, R. A.; Straley, J. M.; Vollenweider, J. J.

    2018-01-01

    We modeled the biomass of Pacific herring (Clupea pallasii) consumed by humpback whales (Megaptera novaeangliae) to determine if whales are preventing the recovery of some herring populations in the Gulf of Alaska. We estimated consumption, by whales, of two depressed (Lynn Canal, Prince William Sound) and one robust (Sitka Sound) herring populations during fall/winter of 2007-2008 and 2008-2009. Consumption estimates relied on observations of whale abundance, prey selection, and herring energy content along with published data on whale size and metabolic rate. Herring biomass removed by whales was compared with independent estimates of herring abundance to assess the impact of predation on each population. Whales removed a greater proportion of the total biomass of herring available in Lynn Canal and Prince William Sound than in Sitka Sound. Biomass removals were greatest in Prince William Sound where we observed the largest number of whales foraging on herring. The biomass of herring consumed in Prince William Sound approximated the biomass lost to natural mortality over winter as projected by age-structured stock assessments. Though whales also focused their foraging on herring during the fall in Lynn Canal, whales were less abundant resulting in lower estimated consumption rates. Whales were more abundant in Sitka Sound than in Lynn Canal but foraged predominately on euphausiids. Herring abundance was greater in Sitka Sound, further reducing the overall impact on the herring population. These data indicate that the focused predation in Prince William Sound can exert top-down controlling pressure, but whale populations are not a ubiquitous constraint on forage fish productivity in the Gulf of Alaska at this time.

  1. Biodiesel production from waste cooking oil using calcined scallop shell as catalyst

    International Nuclear Information System (INIS)

    Sirisomboonchai, Suchada; Abuduwayiti, Maidinamu; Guan, Guoqing; Samart, Chanatip; Abliz, Shawket; Hao, Xiaogang; Kusakabe, Katsuki; Abudula, Abuliti

    2015-01-01

    Highlights: • Calcined scallop shell was used as low-cost and effective catalyst for biodiesel production. • BDF yield from waste cooking oil reached 86% at 65 °C with a catalyst loading amount of 5 wt%. • Calcined scallop shell showed good reusability. • Calcium glyceroxide played an important role on the reusability of calcined scallop shell. • Water in the waste cooking oil had negative effect on the catalytic activity of calcined scallop shell. - Abstract: Transesterification of waste cooking oil (WCO) and methanol by using calcined scallop shell (CSS) as catalyst was carried out in a closed system for biodiesel fuel (BDF) production. It is found that the optimum calcination temperature for the preparation of CSS was 1000 °C. The effects of transesterification temperature, reaction time, methanol/oil molar ratio and catalyst loading amount on the BDF yield were investigated. Compared with the commercial CaO, CSS showed higher catalytic activity and the BDF yield reached 86% at 65 °C with a catalyst loading amount of 5 wt% (WCO basis) and a reaction time of 2 h. The catalyst was reused for 5 cycles whilst the BDF yield decreased 23%. It is found that CaO in CSS was transferred to calcium glyceroxide after the transesterification reaction, and calcium glyceroxide also showed good catalytic activity and reusability. Furthermore, Water content in WCO had negative effect on BDF yield. It is found that BDF yield reduced 15% due to the occurring of saponification when the water content was increased from 0.64% to 2.48%. It is expected that CCS can be used as an alternative and cheap catalyst for the biodiesel production

  2. Impact of Air Frying on Cholesterol and Fatty Acids Oxidation in Sardines: Protective Effects of Aromatic Herbs.

    Science.gov (United States)

    Ferreira, Fernanda S; Sampaio, Geni R; Keller, Laura M; Sawaya, Alexandra C H F; Chávez, Davy W H; Torres, Elizabeth A F S; Saldanha, Tatiana

    2017-12-01

    The high temperatures used to fry fish may induce thermo-oxidation of cholesterol, forming cholesterol oxidation products (COPs). COPs have been associated to coronary heart diseases, atherosclerosis, and other chronic diseases. Air fryers are an alternative thermal process technology to fry foods without oil, and are considered a healthier cooking method. This study is the 1st to evaluate the formation of COPs and the degradation of polyunsaturated fatty acids (PUFAs) in air-fried sardine fillets. Furthermore, we evaluated the effect of fresh herbs added as natural antioxidants to sardines subjected to air frying. Parsley (Petroselinum crispum), chives (Allium schoenoprasum L.), and a mixture of both herbs (cheiro-verde) were added in quantities of 0%, 2%, and 4%. Air frying significantly decreased the content of essential PUFAs, and increased the levels of COPs from 61.2 (raw) to 283 μg/g (P addition of 4% of cheiro-verde in air-fried sardines presented the best protective effect against lipid oxidation. Fish is an important source of essential lipids. However, oxidized cholesterol products, which are formed during thermal processing, are potential hazards to human health. Air fryers present an alternative thermal process for frying food without oil, and this method of cooking is considered to be more convenient and healthier This study shows that the air frying increased the formation of cholesterol oxidation products and decreased the essential polyunsaturated fatty acids in sardine fillets. However, the lipid oxidation is significantly reduced by adding fresh herbs, such as parsley (Petroselinum crispum), chives (Allium schoenoprasum L.), or a mixture of both herbs (cheiro-verde) that are natural antioxidants. © 2017 Institute of Food Technologists®.

  3. Seasonal variation in the levels of organohalogen compounds in herring (Clupea harengus) from the Norwegian Sea.

    Science.gov (United States)

    Frantzen, Sylvia; Måge, Amund; Iversen, Svein Arnholt; Julshamn, Kåre

    2011-09-01

    The Norwegian spring spawning (NSS) herring is an ecologically important fish stock in the Norwegian Sea, and with a catch volume exceeding one million tons a year it is also economically important and a valuable food source. In order to provide a baseline of the levels of contaminants in this fish stock, the levels of organohalogen compounds were determined in 800 individual herring sampled at 29 positions in the Norwegian Sea and off the coast of Norway. Due to seasonal migration, the herring were sampled where they were located during the different seasons. Concentrations of dioxins and dioxin-like PCBs, non-dioxin-like PCBs (PCB(7)) and PBDEs were determined in fillet samples of individual herring, and found to be relatively low, with means (min-max) of 0.77 (0.24-3.5) ng TEQ kg(-1) wet weight (ww), 5.0 (1.4-24) μg kg(-1) ww and 0.47 (0.091-3.1) μg kg(-1) ww, respectively. The concentrations varied throughout the year due to the feeding- and spawning cycle: Starved, pre-spawning herring caught off the Norwegian coast in January-February had the highest levels and those caught in the Norwegian Sea in April-June, after further starvation and spawning, had the lowest levels. These results show that the concentrations of organohalogen compounds in NSS herring are relatively low and closely tied to their physiological condition, and that in the future regular monitoring of NSS herring should be made in the spawning areas off the Norwegian coast in late winter. Copyright © 2011 Elsevier Ltd. All rights reserved.

  4. Precision and relative effectiveness of a purse seine for sampling age-0 river herring in lakes

    Science.gov (United States)

    Devine, Matthew T.; Roy, Allison; Whiteley, Andrew R.; Gahagan, Benjamin I.; Armstrong, Michael P.; Jordaan, Adrian

    2018-01-01

    Stock assessments for anadromous river herring, collectively Alewife Alosa pseudoharengus and Blueback Herring A. aestivalis, lack adequate demographic information, particularly with respect to early life stages. Although sampling adult river herring is increasingly common throughout their range, currently no standardized, field‐based, analytical methods exist for estimating juvenile abundance in freshwater lakes. The objective of this research was to evaluate the relative effectiveness and sampling precision of a purse seine for estimating densities of age‐0 river herring in freshwater lakes. We used a purse seine to sample age‐0 river herring in June–September 2015 and June–July 2016 in 16 coastal freshwater lakes in the northeastern USA. Sampling effort varied from two seine hauls to more than 50 seine hauls per lake. Catch rates were highest in June and July, and sampling precision was maximized in July. Sampling at night (versus day) in open water (versus littoral areas) was most effective for capturing newly hatched larvae and juveniles up to ca. 100 mm TL. Bootstrap simulation results indicated that sampling precision of CPUE estimates increased with sampling effort, and there was a clear threshold beyond which increased effort resulted in negligible increases in precision. The effort required to produce precise CPUE estimates, as determined by the CV, was dependent on lake size; river herring densities could be estimated with up to 10 purse‐seine hauls (one‐two nights) in a small lake (50 ha). Fish collection techniques using a purse seine as described in this paper are likely to be effective for estimating recruit abundance of river herring in freshwater lakes across their range.

  5. Novel species interactions: American black bears respond to Pacific herring spawn.

    Science.gov (United States)

    Fox, Caroline Hazel; Paquet, Paul Charles; Reimchen, Thomas Edward

    2015-05-26

    In addition to the decline and extinction of the world's species, the decline and eventual loss of species interactions is one of the major consequences of the biodiversity crisis. On the Pacific coast of North America, diminished runs of salmon (Oncorhynchus spp.) drive numerous marine-terrestrial interactions, many of which have been intensively studied, but marine-terrestrial interactions driven by other species remain relatively unknown. Bears (Ursus spp.) are major vectors of salmon into terrestrial ecosystems, but their participation in other cross-ecosystem interactions is similarly poorly described. Pacific herring (Clupea pallasii), a migratory forage fish in coastal marine ecosystems of the North Pacific Ocean and the dominant forage fish in British Columbia (BC), spawn in nearshore subtidal and intertidal zones. Spawn resources (eggs, milt, and spawning adults) at these events are available to coastal predators and scavengers, including terrestrial species. In this study, we investigated the interaction between American black bears (Ursus americanus) and Pacific herring at spawn events in Quatsino Sound, BC, Canada. Using remote cameras to monitor bear activity (1,467 camera days, 29 sites, years 2010-2012) in supratidal and intertidal zones and a machine learning approach, we determined that the quantity of Pacific herring eggs in supratidal and intertidal zones was a leading predictor of black bear activity, with bears positively responding to increasing herring egg masses. Other important predictors included day of the year and Talitrid amphipod (Traskorchestia spp.) mass. A complementary analysis of black bear scats indicated that Pacific herring egg mass was the highest ranked predictor of egg consumption by bears. Pacific herring eggs constituted a substantial yet variable component of the early springtime diet of black bears in Quatsino Sound (frequency of occurrence 0-34%; estimated dietary content 0-63%). Other major dietary items included

  6. Proteolysis of Sardine (Sardina pilchardus and Anchovy (Stolephorus commersonii by Commercial Enzymes in Saline Solutions

    Directory of Open Access Journals (Sweden)

    Chau Minh Le

    2015-01-01

    Full Text Available Fish sauce production is a very long process and there is a great interest in shortening it. Among the different strategies to speed up this process, the addition of external proteases could be a solution. This study focuses on the eff ect of two commercial enzymes (Protamex and Protex 51FP on the proteolysis of two fish species traditionally converted into fish sauce: sardine and anchovy, by comparison with classical autolysis. Hydrolysis reactions were conducted with fresh fish at a temperature of 30 °C and under different saline conditions (from 0 to 30 % NaCl. Hydrolysis degree and liquefaction of the raw material were used to follow the process. As expected, the proteolysis decreased with increasing amount of salt. Regarding the fi sh species, higher rate of liquefaction and higher hydrolysis degree were obtained with anchovy. Between the two proteases, Protex 51FP gave better results with both fi sh types. This study demonstrates that the addition of commercial proteases could be helpful for the liquefaction of fi sh and cleavage of peptide bonds that occur during fi sh sauce production and thus speed up the production process.

  7. [Evaluation of prerequisites programs for a HACCP plan for frozen sardine plant].

    Science.gov (United States)

    Rosas, Patricia; Reyes, Genara

    2008-06-01

    Good manufacturing practices (GMP) and sanitation standard operating procedures (SSOP) are prerequisites programs for the application of the Hazard Analysis and Critical Control Point (HACCP) system as a food safety approach during processing. The aim of this study was to evaluate GMP/SSOP prerequisites in processing line of frozen whole sardine (Sardinella aurita). The GMP compliance was verified according to a standard procedure of the Ministry for the Health of Venezuela, and the SSOP were assessed according to a checklist proposed by the FDA. GMP and SSOP were evaluated following a demerit-based approach. A percentage value was calculated and referred to as sanitary effectiveness. Results indicated that the plant had a good level of compliance with GMP from assessment of buildings and facilities, equipment and tools, hygienic requisites of the production, assurance of the hygiene quality, storage and transportation, and the percentage of sanitary effectiveness was 84%. The level of compliance for SSOP was 53,12% with demerits found in all assessed aspects consisting of inexistent guidelines, lack of control in the sanitary plan and lack of leadership in applying corrective actions. Thus, an improvement in the plant sanitation program was designed targeting SSOP.

  8. Outlier Loci Detect Intraspecific Biodiversity amongst Spring and Autumn Spawning Herring across Local Scales.

    Directory of Open Access Journals (Sweden)

    Dorte Bekkevold

    Full Text Available Herring, Clupea harengus, is one of the ecologically and commercially most important species in European northern seas, where two distinct ecotypes have been described based on spawning time; spring and autumn. To date, it is unknown if these spring and autumn spawning herring constitute genetically distinct units. We assessed levels of genetic divergence between spring and autumn spawning herring in the Baltic Sea using two types of DNA markers, microsatellites and Single Nucleotide Polymorphisms, and compared the results with data for autumn spawning North Sea herring. Temporally replicated analyses reveal clear genetic differences between ecotypes and hence support reproductive isolation. Loci showing non-neutral behaviour, so-called outlier loci, show convergence between autumn spawning herring from demographically disjoint populations, potentially reflecting selective processes associated with autumn spawning ecotypes. The abundance and exploitation of the two ecotypes have varied strongly over space and time in the Baltic Sea, where autumn spawners have faced strong depression for decades. The results therefore have practical implications by highlighting the need for specific management of these co-occurring ecotypes to meet requirements for sustainable exploitation and ensure optimal livelihood for coastal communities.

  9. Latitudinal Gradient in Otolith Shape among Local Populations of Atlantic Herring (Clupea harengus L.) in Norway.

    Science.gov (United States)

    Libungan, Lísa Anne; Slotte, Aril; Husebø, Åse; Godiksen, Jane A; Pálsson, Snæbjörn

    2015-01-01

    Otolith shape analysis of Atlantic herring (Clupea harengus) in Norwegian waters shows significant differentiation among fjords and a latitudinal gradient along the coast where neighbouring populations are more similar to each other than to those sampled at larger distances. The otolith shape was obtained using quantitative shape analysis, the outlines were transformed with Wavelet and analysed with multivariate methods. The observed morphological differences are likely to reflect environmental differences but indicate low dispersal among the local herring populations. Otolith shape variation suggests also limited exchange between the local populations and their oceanic counterparts, which could be due to differences in spawning behaviour. Herring from the most northerly location (69°N) in Balsfjord, which is genetically more similar to Pacific herring (Clupea pallasii), differed in otolith shape from all the other populations. Our results suggest that the semi-enclosed systems, where the local populations live and breed, are efficient barriers for dispersal. Otolith shape can thus serve as a marker to identify the origin of herring along the coast of Norway.

  10. Predation on larval Atlantic herring (Clupea harengus) in inshore waters of the Baltic Sea

    Science.gov (United States)

    Kotterba, Paul; Moll, Dorothee; von Nordheim, Lena; Peck, Myron A.; Oesterwind, Daniel; Polte, Patrick

    2017-11-01

    In fishery science, early life-stage survival and development are regarded as major factors driving the population dynamics of marine fishes. During the last century, the main research focus has been on the spatio-temporal match of larval fish and appropriate food (bottom-up processes). However, these field studies are often criticised for their limited capability to disentangle their results from mortality caused by predation since these top-down mechanisms are rarely studied. We examined the predation on herring (Clupea harengus) larvae in a Baltic inshore lagoon by investigating the spatio-temporal overlap of larval herring and their potential predators such as the dominant threespine stickleback (Gasterosteus aculeatus) in distinct habitats (sublittoral and littoral areas) using a set of different gears and sampling techniques. Despite significant spatial and temporal predator-prey overlap, stomach analyses suggested that very few larvae were consumed by sticklebacks, even if projected to the entire study area and season. Other well-known predators of clupeid larvae such as gelatinous plankton occur later in the year after young herring have migrated out of the system. The observed predation on herring larvae was much less than expected and appears being a minor factor in determining herring reproduction success in our study area, particularly if compared to other causes of mortality such as egg predation. Providing a relatively good shelter from predation might be a key element making transitional waters valuable nursery grounds for the offspring of migrating marine fish species.

  11. Herring parasite and tissue alterations following the Exxon Valdez oil spill

    International Nuclear Information System (INIS)

    Moles, A.D.; Rice, S.D.; Okihiro, M.S.

    1993-01-01

    The authors examined the intensity and prevalence of larval nematodes (Anisakis simplex) and alterations in selected tissues of spawning Pacific herring (Clupea harengus pallasi) exposed to crude oil, in the laboratory under controlled conditions and in Prince William Sound 14 days after the Exxon Valdez oil spill. In the laboratory, intensity and prevalence of nematodes in the body cavities of herring exposed to the water-soluble fraction of oil declined when exposed to doses above 1.2 mg/L total aromatics. In Prince William Sound, nematodes were rare in spawning herring from oiled sites and abundant among herring from areas outside the spill. Oil exposure apparently induced the nematodes to migrate from the body cavity to the body wall with the lower intensity reflecting a change in parasite location. A coccidian, Eimeria clupearum, was found in greater numbers in oil-exposed herring. To verify exposure effects and to link parasite and tissue alteration with oil exposure, histological examination was used. Liver coagulative necrosis indicated hepatotoxic exposure. Necrosis was followed by macrophage aggregation in the resolution phase. The laboratory exposures allowed confirmation of oil exposure in Prince William Sound and permitted analysis of effects on two internal parasites

  12. Feasibility of Acoustic Remote Sensing of Large Herring Shoals and Seafloor by Baleen Whales

    Directory of Open Access Journals (Sweden)

    Dong Hoon Yi

    2016-08-01

    Full Text Available Recent research has found a high spatial and temporal correlation between certain baleen whale vocalizations and peak herring spawning processes in the Gulf of Maine. These vocalizations are apparently related to feeding activities with suggested functions that include communication, prey manipulation, and echolocation. Here, the feasibility of the echolocation function is investigated. Physical limitations on the ability to detect large herring shoals and the seafloor by acoustic remote sensing are determined with ocean acoustic propagation, scattering, and statistical theories given baleen whale auditory parameters. Detection is found to be highly dependent on ambient noise conditions, herring shoal distributions, baleen whale time-frequency vocalization spectra, and geophysical parameters of the ocean waveguide. Detections of large herring shoals are found to be physically feasible in common Gulf of Maine herring spawning scenarios at up to 10 ± 6 km in range for humpback parameters and 1 ± 1 km for minke parameters but not for blue and fin parameters even at zero horizontal range. Detections of the seafloor are found to be feasible up to 2 ± 1 km for blue and humpback parameters and roughly 1 km for fin and minke parameters, suggesting that the whales share a common acoustic sensation of rudimentary features of the geophysical environment.

  13. Latitudinal Gradient in Otolith Shape among Local Populations of Atlantic Herring (Clupea harengus L.) in Norway

    Science.gov (United States)

    Libungan, Lísa Anne; Slotte, Aril; Husebø, Åse; Godiksen, Jane A.; Pálsson, Snæbjörn

    2015-01-01

    Otolith shape analysis of Atlantic herring (Clupea harengus) in Norwegian waters shows significant differentiation among fjords and a latitudinal gradient along the coast where neighbouring populations are more similar to each other than to those sampled at larger distances. The otolith shape was obtained using quantitative shape analysis, the outlines were transformed with Wavelet and analysed with multivariate methods. The observed morphological differences are likely to reflect environmental differences but indicate low dispersal among the local herring populations. Otolith shape variation suggests also limited exchange between the local populations and their oceanic counterparts, which could be due to differences in spawning behaviour. Herring from the most northerly location (69°N) in Balsfjord, which is genetically more similar to Pacific herring (Clupea pallasii), differed in otolith shape from all the other populations. Our results suggest that the semi-enclosed systems, where the local populations live and breed, are efficient barriers for dispersal. Otolith shape can thus serve as a marker to identify the origin of herring along the coast of Norway. PMID:26101885

  14. Otolith shape: a population marker for Atlantic herring Clupea harengus.

    Science.gov (United States)

    Libungan, L A; Óskarsson, G J; Slotte, A; Jacobsen, J A; Pálsson, S

    2015-04-01

    Otolith shape variation of seven Atlantic herring Clupea harengus populations from Canada, the Faroe Islands, Iceland, Ireland, Norway and Scotland, U.K., covering a large area of the species' distribution, was studied in order to see if otolith shape can be used to discriminate between populations. The otolith shape was obtained using quantitative shape analysis, transformed with Wavelet and analysed with multivariate methods. Significant differences were detected among the seven populations, which could be traced to three morphological structures in the otoliths. The differentiation in otolith shape between populations was not only correlated with their spawning time, indicating a strong environmental effect, but could also be due to differing life-history strategies. A model based on the shape differences discriminates with 94% accuracy between Icelandic summer spawners and Norwegian spring spawners, which are known to mix at feeding grounds. This study shows that otolith shape could become an accurate marker for C. harengus population discrimination. © 2015 The Fisheries Society of the British Isles.

  15. Nutritional value and acceptability of homemade maize/sorghum-based weaning mixtures supplemented with rojo bean flour, ground sardines and peanut paste.

    Science.gov (United States)

    Mosha, Theobald C E; Vicent, Mary M

    2004-06-01

    Low nutrient density in weaning foods is the major cause of under-nutrition among infants and young children in developing countries. Ten types of composite weaning diets (namely, maize-rojo beans-peanut, maize-peanut-sardines, maize-peanut-sardine-rojo beans, maize-peanut-soaked rojo beans, maize-peanut-germinated rojo beans, sorghum-rojo beans-peanut, sorghum-peanut-sardines, sorghum-peanut-sardine-rojo beans, sorghum-peanut-soaked rojo beans, and sorghum-peanut-germinated rojo beans) were formulated and assayed for proximate composition, energy, mineral density, tannin content and residual urease activity. The diets were also evaluated for storage stability under ambient conditions, sensory quality and overall acceptability. Results of the study indicated that, concentrations of protein, fat, ash, calcium, iron, zinc and copper were significantly (Pfoods. Both maize and sorghum-based composite gruels had a short shelf-life under ambient conditions (26.4 degrees C) ranging between 4 and 6 h, with gruels containing ground sardines showing a tendency to spoil faster. All composite gruels except those containing germinated rojo beans were highly liked and accepted by consumers (Pfoods for older infants and young children. Further investigations are suggested to extend the shelf-life of the composite products and improve the organoleptic quality of the diets containing germinated rojo beans.

  16. Spatially explicit estimates of stock sizes, structure and biomass of herring and blue whiting, and catch data of bluefin tuna

    DEFF Research Database (Denmark)

    Huse, G.; MacKenzie, B. R.; Trenkel, V.

    2015-01-01

    The North Atlantic is a productive marine region which has supported important commercial fisheries for centuries. Many of these fisheries have exploited the pelagic species, including herring, blue whiting and tuna. Here we present data on the distribution of herring and blue whiting based on th...

  17. Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri

    Institute of Scientific and Technical Information of China (English)

    ZHANG Yang; ZHANG Xiaojun; Chantel F.SCHEURING; ZHANG Hongbin; LI Fuhua; XIANG Jianhai

    2008-01-01

    Two Large-insert genomic bacterial artificial chromosome (BAC) libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research.High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I,respectively.The BamH I library consisted of 53760 clones while the Mbo I library consisted of 7680 clones.Approximately 96% of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb,providing a coverage of 5.3 haploid genome equivalents.Similarly,the Mbo I library with an average insert of 145 kb and no insert-empty clones,thus providing a genome coverage of 1.1 haploid genome equivalents.

  18. Extrinsic and intrinsic factors that shape the life history of the short living scallop Argopecten ventricosus

    OpenAIRE

    Guerra, Citlali

    2011-01-01

    The scallop Argopecten ventricosus is characterized by its high swimming activity, fast growth, high reproductive effort and the early age to get first sexual maturity. These traits may be the result of the adaptation to a specific environment that favors an active lifestyle and a short lifespan (2 years). This opens the question of how environmental factors modulate the way a short living marine ectotherm budget energy investments among life history traits and how this modulation impacts the...

  19. Larval growth and drift pattern and the separation of herring spawning groups in the North Sea

    DEFF Research Database (Denmark)

    Munk, Peter; Christensen, Villy

    1990-01-01

    Data from two internationally co-ordinated survey programmes are used in an attempt to display the events in the early life that took place during the 'recovery period' of the North Sea herring stock, 1979-1986. During the period, an increasing importance of the spawning grounds off Buchan and off...... Yorkshire is evident from distributions of newly hatched herring larvae. The dramatic changes in relative importance of spawning grounds are traced in the data on both late larvae (6 months old) and juveniles (18 months old). The onset of extended spawning off Buchan and off Yorkshire was followed...... in the relative contributions from spawning grounds apparently influence the overall spatial distribution as well as size composition of larval and juvenile North Sea herring. The findings indicate that groups of larvae retain, to a large extent, separate distributions until metamorphosis, and point to larval...

  20. Stock structure of Atlantic herring Clupea harengus in the Norwegian Sea and adjacent waters

    DEFF Research Database (Denmark)

    Pampoulie, Christophe; Slotte, Aril; Oskarsson, Guomundur J.

    2015-01-01

    The genetic structure of Atlantic herring Clupea harengus L. was investigated in its north-easterly distribution in the Norwegian Sea and adjacent waters, using 23 neutral and one non-neutral (Cpa111) microsatellite loci. Fish from the suspected 2 main populations-the Norwegian spring......-spawning herring (NSSH) and the Icelandic summer-spawning herring (ISSH)-were collected at spawning locations in their respective spawning seasons from 2009 to 2012. Samples were also collected from Norwegian autumn spawning locations, from different local Norwegian fjords such as the inner part...... of Trondheimsfjorden, Lindas pollene, Landvikvannet and Lusterfjorden, as well as from suspected Faroese spawning components. The observed level of genetic differentiation was significant but low (F-ST = 0.007) and mostly attributable to the differentiation of the local Norwegian fjord populations. The locus Cpa111...

  1. Incidence of Ichthyophonus hoferi in Puget Sound fishes and its increase with age of Pacific herring

    Science.gov (United States)

    Hershberger, P.K.; Stick, K.; Bui, B.; Carroll, C.; Fall, B.; Mork, C.; Perry, J.A.; Sweeney, E.; Wittouck, J.; Winton, J.; Kocan, R.

    2002-01-01

    A recent decrease in the mean age of adult Pacific herring Clupea pallasi in Puget Sound was associated with a high prevalence of Ichthyophonus hoferi, a protistan parasite that can be highly pathogenic to Pacific herring. In Puget Sound, high intensities of I. hoferiinfection may be maintained in older cohorts of Pacific herring because the prevalence ofI. hoferi increased with age from 12% among juveniles to 58% among the oldest, age-6 and older cohorts. Low intensities of I. hoferi infection in the region may be maintained in alternative fish hosts, such as surf smelt Hypomesus pretiosus, Puget Sound rockfishSebastes emphaeus, Pacific tomcod Microgadus proximus, and speckled sanddabCithanichthys stigmaeus.

  2. Exxon Valdez oil spill restoration project final report: Prince William Sound Herring disease program (HDP), restoration project 070819

    Science.gov (United States)

    Hershberger, Paul; Elliott, Diane G.; Emmenegger, Eveline J.; Hansen, John D.; Kurath, Gael; Winton, James R.; Kocan, Richard; LaPatra, Scott

    2011-01-01

    Surveys of pathogens in Pacific herring from 2007 – 2010 indicated that Ichthyophonus, viral hemorrhagic septicemia virus, and erythrocytic necrosis virus are endemic in Prince William Sound and throughout the NE Pacific. Laboratory studies with VHSV indicated that multiple herring stocks are equally susceptible to the resulting disease, Pacific herring shed copious levels of VHSV (as high at 5x108 plaque-forming units/day) shortly after exposure, chronic and persistent infections can occur in Pacific herring, susceptibility of Pacific herring to VHS extends to the larval life stages but not the embryonic stages, and the prior exposure history of Pacific herring to VHSV can be determined post hoc. Laboratory studies involving Ichthyophonus indicated that schizonts can be inactivated with chlorine and iodine solutions, the parasite can survive for extended periods in saltwater but not freshwater, a low potential exists for cross contamination between in vitro explant cultures, infectious schizonts are released from the skin surface of infected herring, schizonts are not uniformly distributed throughout the skeletal muscle of infected Pacific herring, multiple types of Ichthyophonus exist with different genotypes and phenotypic traits, and temperature is an important factor influencing the infectivity of Ichthyophonus. Additional field and laboratory studies indicated that Ichthyophonus negatively influences the swimming performance of infected hosts and the negative impacts effects are exacerbated by increasing temperatures, American shad are an important reservoir of Ichthyophonus in the NE Pacific, Pacific herring are not susceptible to infectious hematopoietic necrosis (IHN), and Pacific herring will accept surgically implanted acoustic tags with negligible impacts on survival.

  3. What is hiding behind ontogenic d13C variations in mollusk shells: New insights from scallops.

    Science.gov (United States)

    Chauvaud, L.; Lorrain, A.; Gillikin, D. P.; Thebault, J.; Paulet, Y.; Strand, O.; Blamart, D.; Guarini, J.; Clavier, J.

    2008-12-01

    We examined d13Ccalcite variations along scallop shells (Pecten maximus) sampled in Norway, France and Spain. Time series of shell calcite d13C show a consistent pattern of decreasing d13C with age. This almost linear d13C trend reflects an increasing contribution of metabolic CO2 to skeletal carbonate throughout ontogeny. We have removed this ontogenic trend to try to extract other information from our shell calcite d13C dataset. Scallops from the Bay of Brest (western Brittany, France) were then used to interpret the data as many environmental parameters were available for this site. d13Ccalcite variations were compared to d13C of dissolved inorganic carbon (DIC) and Chl a. The detrended calcite d13C profiles seem to follow a seasonal pattern, but surprisingly are inversely related to the d13C DIC and chlorophyll a concentrations measured within the water column. Theses results suggest that shell d13C variations are not controlled by isotopic variation of DIC. Since scallops eat phytoplankton and microphytobenthos cells, and, as a consequence respire organic mater largely depleted in 13C, we therefore suggest that in mollusk suspension feeders the shell d13Ccalcite might still be used to track the annual number of phytoplankton blooms when d13C values of calcite are detrended. We must consider this trend as a potential biological filter hiding precious environmental records.

  4. Viral tropism and pathology associated with viral hemorrhagic septicemia in larval and juvenile Pacific herring

    Science.gov (United States)

    Lovy, Jan; Lewis, N.L.; Hershberger, P.K.; Bennett, W.; Meyers, T.R.; Garver, K.A.

    2012-01-01

    Viral hemorrhagic septicemia virus (VHSV) genotype IVa causes mass mortality in wild Pacific herring, a species of economic value, in the Northeast Pacific Ocean. Young of the year herring are particularly susceptible and can be carriers of the virus. To understand its pathogenesis, tissue and cellular tropisms of VHSV in larval and juvenile Pacific herring were investigated with immunohistochemistry, transmission electron microscopy, and viral tissue titer. In larval herring, early viral tropism for epithelial tissues (6d post-exposure) was indicated by foci of epidermal thickening that contained heavy concentrations of virus. This was followed by a cellular tropism for fibroblasts within the fin bases and the dermis, but expanded to cells of the kidney, liver, pancreas, gastrointestinal tract and meninges in the brain. Among wild juvenile herring that underwent a VHS epizootic in the laboratory, the disease was characterized by acute and chronic phases of death. Fish that died during the acute phase had systemic infections in tissues including the submucosa of the gastrointestinal tract, spleen, kidney, liver, and meninges. The disease then transitioned into a chronic phase that was characterized by the appearance of neurological signs including erratic and corkscrew swimming and darkening of the dorsal skin. During the chronic phase viral persistence occurred in nervous tissues including meninges and brain parenchymal cells and in one case in peripheral nerves, while virus was mostly cleared from the other tissues. The results demonstrate the varying VHSV tropisms dependent on the timing of infection and the importance of neural tissues for the persistence and perpetuation of chronic infections in Pacific herring.

  5. Food safety considerations in relation to Anisakis pegreffii in anchovies (Engraulis encrasicolus) and sardines (Sardina pilchardus) fished off the Ligurian Coast (Cinque Terre National Park, NW Mediterranean).

    Science.gov (United States)

    Serracca, Laura; Battistini, Roberta; Rossini, Irene; Carducci, Annalaura; Verani, Marco; Prearo, Marino; Tomei, Laura; De Montis, Gabriella; Ercolini, Carlo

    2014-11-03

    Engraulis encrasicolus and Sardina pilchardus are pelagic fishes of notable economic and gastronomic importance in the northwest Mediterranean (Ligurian Sea, Italy). The consumption of thermally unprocessed or lightly processed, marinated or salted anchovies and sardines presents a potential risk to acquire anisakiasis, a fish-borne parasitic disease in humans. Prevalence and abundance of Anisakis larvae in Engraulis encrasicolus and Sardina pilchardus from the Monterosso fishing grounds (Cinque Terre National Park, Ligurian Sea, Italy) were assessed, and the larvae were identified by morphological and PCR-RFLP methods. Anisakis larvae, all belonging to Anisakis pegreffii spp. were found in the visceral mass of 1050 anchovies (0.8% overall prevalence), whereas no Anisakis larvae were found in the 750 sardines examined. According to these data, the risk of acquiring anisakiasis from the consumption of raw or undercooked anchovies and sardines caught in the fishing area we investigated is very low. Copyright © 2014 Elsevier B.V. All rights reserved.

  6. Framing the problem with stakeholders: five views to herring fishery management

    DEFF Research Database (Denmark)

    Haapasaari, Päivi Elisabet; Mäntyniemi, Samu; Kuikka, Sakari

    Fisheries management is often subject to disputes due to high uncertainty and high values at stake. Such disputes call for improving the communication between parties, the understanding of policy processes, and transparency. We hypothesize that a transparent basis for a dialogue could be built...... should be taken into account in herring management, 2) Which variables are of primary interest, 3) What should be the objectives for herring management, and 4) What kind of management measures could or should be used to reach these objectives. The views of the stakeholders were built into causal maps...

  7. Chemical Characterization, Antioxidant and Enzymatic Activity of Brines from Scandinavian Marinated Herring Products

    DEFF Research Database (Denmark)

    Gringer, Nina; Osman, Ali; Nielsen, Henrik Hauch

    2014-01-01

    Brines generated during the last marination step in the production of marinated herring (Clupea harengus) were chemically characterized and analyzed for antioxidant and enzyme activities. The end-products were vinegar cured, spice cured and traditional barrel-salted herring with either salt...... or spices. The chemical characterization encompassed pH, dry matter, ash, salt, fatty acids, protein, polypeptide pattern, iron and nitrogen. The antioxidant activity was tested with three assays measuring: iron chelation, reducing power and radical scavenging activity. The enzymatic activity for peroxidase...

  8. Brazilian sardine (Sardinella brasiliensis spawning in the southeast Brazilian Bight over the period 1976-1993

    Directory of Open Access Journals (Sweden)

    Yasunobu Matsuura

    1998-01-01

    Full Text Available Based on sampling over the period 1976-1993 in the southeast Brazilian Bight, the distribution of spawning of the Brazilian sardine (Sardinella brasi/iensis is described in relation to environmental conditions. The area of intense spawning occurs in the southern part of the bight where coastal upwelling was less /Tequent. Spawning intensity showed high interannllal variation and the egg abundance in the survey area ranged /Tom 99 billion eggs in the January 1988 cruise to 4669 billion eggs in the January 1981 cruise. Peak spawning takes place one hour after midnight and eggs hatch . out within 19 hours with a water temperature of 24 °e.Baseado nos dados coletados durante nove cruzeiros oceanográficos realizados na região sudeste, as áreas de desova da sardinha-verdadeira (Sardinella brasiliensis foram apresentadas c discutidas em relação às condições oceanográficas. As áreas de desova intensiva foram localizadas na parte sul da área de investigação, onde a ressurgência costeira foi menos freqüente. A intensidade de desova demonstrou uma variação anual relativamente grande. A produção total de ovos da sardinha- ­verdadeira variou de 99 bilhões de ovos durante o cruzeiro de janeiro de 1988 para 4669 bilhões de ovos em janeiro de 1981. O pico de desova ocorre na camada de mistura de superfície uma hora após a meia noite e os ovos eclodem em 19 horas com a temperatura de água 24 °e.

  9. Food and feeding ecology of Brazilian sardine (Sardinella brasiliensis larvae from the southeastern Brazilian Bight

    Directory of Open Access Journals (Sweden)

    Frederico W. Kurtz

    2001-01-01

    Full Text Available Results from depth integrated and vertically stratified plankton samples collected in the southeastern Brazilian Bight were used to study the feeding behavior of Brazilian sardine (Sardinella brasiliensis larvae. Sampling of the ichthyoplankton was carried out with 60 cm Bongo nets in the Bight during the spawning seasons of 1991/92 and 1992/93. The sampling of microzooplankton was carried out in the coastal region off Ubatuba, using the closing-type plankton net, in December 1995. The feeding study was based on a total of 901 captured larvae. Gut content analysis of the sardine larvae showed a diurnal pattern of food intake. Copepod nauplii dominated the diet of the preflexion and flexion larvae, but they were the second in abundance for the postflexion larvae which fed preferentially on copepodites and adults of Oncaea spp. Averaged feeding incidence of the 901 larvae was 37.6%, but it increased to 58.5% for day-caught larvae. Seventy percent of the food particles were found in the mid-gut and food eaten showed a natural increase in digestion from fore-gut to hind-gut. Vertical distribution of microzooplankton revealed that copepod nauplii were present in densities of 10-20 ind. L-1, mainly in the upper mixed layer (0-20 m depth, but higher densities of copepodite and adult of Oncaea, Oithona and Paracalamis were found within and beneath the thermocline. These results show that Brazilian sardine larvae can successfully adapt their diet, feeding on the most abundant food particles in the upper mixed layer of the survey area.Plâncton amostrado na região sudeste do Brasil, em coletas integradas verticalmente e estratificadas, foi utilizado no estudo do comportamento alimentar das larvas de sardinha-verdadeira (Sardinella brasiliensis. As coletas de ictioplâncton foram realizadas com rede tipo Bongo de 60 cm de diâmetro durante os períodos de desova de 1991/92 e 1992/93. Microzooplâncton foi amostrado na região costeira ao largo de Ubatuba

  10. Effects of the Exxon Valdez oil spill on survival of Pacific herring eggs and viability of their larvae

    International Nuclear Information System (INIS)

    McGurk, M.D.; Warburton, H.D.; Parker, T.B.; Litke, M.; Marliave, J.B.

    1993-01-01

    The Exxon Valdez spill in March 1989 coincided with the spawning period of Pacific herring (Clupea pallasi) in the spill area. Adult herring were first observed near their spawning ground about a week after the spill. Spawners were concentrated in four major areas of Prince William Sound. The northeast and north areas were untouched by the spill, but the Naked Island archipelago and the northern tip of Montague Island were in the spill path. Previous research has shown that growth and mortality of free-swimming herring larvae were not significantly different between non-oiled and oiled areas of the Sound, suggesting that any effect of the oil spill on herring may have been restricted to the egg stage. This hypothesis was tested by measuring survival, hatching schedule, and viability of herring eggs collected from oiled and non-oiled areas of the Sound and incubated in laboratory aquaria. Variance analysis showed that egg survival and mean age of hatch varied with oil treatment, depth, and the interaction of treatment and depth. Six larval abnormalities were identified, all of which would render larvae effectively dead in a natural environment. Dry egg weight and yolk volume varied only with depth. Despite these findings, the population dynamics of both oiled and non-oiled herring eggs from the Sound in 1989 resembled the dynamics of natural, uncontaminated herring eggs. A possible reason for this similarity is that most eggs in the oil treatments may have been exposed to relatively low concentrations of hydrocarbons. 2 refs

  11. The effect of predator exposure and reproduction on oxidative stress parameters in the Catarina scallop Argopecten ventricosus.

    Science.gov (United States)

    Guerra, C; Zenteno-Savín, T; Maeda-Martínez, A N; Abele, D; Philipp, E E R

    2013-05-01

    Predation is known to impact growth and reproduction, and the physiological state of the prey, including its susceptibility to oxidative stress. In this study, we investigated how prolonged exposure to predators modulates tissue specific antioxidant defense and oxidative damage in the short-lived epibenthic scallop Argopecten ventricosus (2years maximum lifespan). Scallops that were experimentally exposed to predators had not only lower antioxidant capacities (superoxide dismutase and catalase), but also lower oxidative damage (protein carbonyls and TBARS=thiobarbituric acid reactive substances including lipid peroxides) in gills and mantle compared to individuals not exposed to predators. In contrast, oxidative damage in the swimming muscle was higher in predator-exposed scallops. When predator-exposed scallops were on the verge of spawning, levels of oxidative damage increased in gills and mantle in spite of a parallel increase in antioxidant defense in both tissues. Levels of oxidative damage increased also in the swimming muscle whereas muscle antioxidant capacities decreased. Interestingly, post-spawned scallops restored antioxidant capacities and oxidative damage to immature levels, suggesting they can recover from spawning-related oxidative stress. Our results show that predator exposure and gametogenesis modulate oxidative damage in a tissue specific manner and that high antioxidant capacities do not necessarily coincide with low oxidative damage. Copyright © 2013 Elsevier Inc. All rights reserved.

  12. Seasonal presence and potential influence of humpback whales on wintering Pacific herring populations in the Gulf of Alaska

    Science.gov (United States)

    Straley, Janice M.; Moran, John R.; Boswell, Kevin M.; Vollenweider, Johanna J.; Heintz, Ron A.; Quinn, Terrance J., II; Witteveen, Briana H.; Rice, Stanley D.

    2018-01-01

    This study addressed the lack of recovery of Pacific herring (Clupea pallasii) in Prince William Sound, Alaska, in relation to humpback whale (Megaptera novaeangliae) predation. As humpback whales rebound from commercial whaling, their ability to influence their prey through top-down forcing increases. We compared the potential influence of foraging humpback whales on three herring populations in the coastal Gulf of Alaska: Prince William Sound, Lynn Canal, and Sitka Sound (133-147°W; 57-61°N) from 2007 to 2009. Information on whale distribution, abundance, diet and the availability of herring as potential prey were used to correlate populations of overwintering herring and humpback whales. In Prince William Sound, the presence of whales coincided with the peak of herring abundance, allowing whales to maximize the consumption of overwintering herring prior to their southern migration. In Lynn Canal and Sitka Sound peak attendance of whales occurred earlier, in the fall, before the herring had completely moved into the areas, hence, there was less opportunity for predation to influence herring populations. North Pacific humpback whales in the Gulf of Alaska may be experiencing nutritional stress from reaching or exceeding carrying capacity, or oceanic conditions may have changed sufficiently to alter the prey base. Intraspecific competition for food may make it harder for humpback whales to meet their annual energetic needs. To meet their energetic demands whales may need to lengthen their time feeding in the northern latitudes or by skipping the annual migration altogether. If humpback whales extended their time feeding in Alaskan waters during the winter months, the result would likely be an increase in herring predation.

  13. 78 FR 33020 - Fisheries of the Northeastern United States; Atlantic Herring Fishery; Amendment 5

    Science.gov (United States)

    2013-06-03

    ...' consistency with the MSA and other applicable law. Following public comment, NMFS will determine if these... E permits, and vessels declaring herring carrier trips via VMS must notify NMFS Office of Law... accurately report the weight of fish, which is obtained by scale weights and/or volumetric estimates. Because...

  14. Spatial variation in growth, condition and maturation reaction norms of the Baltic herring Clupea harengus membras

    NARCIS (Netherlands)

    Vainikka, A.; Mollet, F.M.; Casini, M.; Gardmark, A.

    2009-01-01

    Understanding of spatial patterns in life-history traits can help fisheries management focus on biologically and functionally relevant stock units. In the present study, we examined life-history variation in growth, condition and maturation of the Baltic herring Clupea harengus membras among

  15. 75 FR 63791 - Fisheries of the Northeastern United States; Atlantic Herring Fishery; Amendment 4

    Science.gov (United States)

    2010-10-18

    ... submit Confidential Business Information or otherwise sensitive or protected information. NMFS will..., uncertainty related to expected catch of herring in the New Brunswick weir fishery and discard [[Page 63793... vessels issued Limited Access Incidental Catch Permits, and 2,272 vessels issued Open Access Permits...

  16. Use of herring bait to farm lobsters in the Gulf of Maine.

    Science.gov (United States)

    Grabowski, Jonathan H; Clesceri, Erika J; Baukus, Adam J; Gaudette, Julien; Weber, Matthew; Yund, Philip O

    2010-04-15

    Ecologists, fisheries scientists, and coastal managers have all called for an ecosystem approach to fisheries management, yet many species such as the American lobster (Homarus americanus) are still largely managed individually. One hypothesis that has yet to be tested suggests that human augmentation of lobster diets via the use of Atlantic herring (Clupea harengus) as bait may contribute to recent increases in lobster landings. Currently 70% of Atlantic herring landings in the Gulf of Maine are used as bait to catch lobsters in traps throughout coastal New England. We examined the effects of this herring bait on the diet composition and growth rate of lobsters at heavily baited vs. seasonally closed (i.e., bait free) sites in coastal Maine. Our results suggest that human use of herring bait may be subsidizing juvenile lobster diets, thereby enhancing lobster growth and the overall economic value and yield of one of the most valuable fisheries in the U.S. Our study illustrates that shifting to an ecosystem approach to fisheries management should require consideration of cross-fishery interactions.

  17. Bayesian stock assessment of Pacific herring in Prince William Sound, Alaska.

    Science.gov (United States)

    Muradian, Melissa L; Branch, Trevor A; Moffitt, Steven D; Hulson, Peter-John F

    2017-01-01

    The Pacific herring (Clupea pallasii) population in Prince William Sound, Alaska crashed in 1993 and has yet to recover, affecting food web dynamics in the Sound and impacting Alaskan communities. To help researchers design and implement the most effective monitoring, management, and recovery programs, a Bayesian assessment of Prince William Sound herring was developed by reformulating the current model used by the Alaska Department of Fish and Game. The Bayesian model estimated pre-fishery spawning biomass of herring age-3 and older in 2013 to be a median of 19,410 mt (95% credibility interval 12,150-31,740 mt), with a 54% probability that biomass in 2013 was below the management limit used to regulate fisheries in Prince William Sound. The main advantages of the Bayesian model are that it can more objectively weight different datasets and provide estimates of uncertainty for model parameters and outputs, unlike the weighted sum-of-squares used in the original model. In addition, the revised model could be used to manage herring stocks with a decision rule that considers both stock status and the uncertainty in stock status.

  18. Climate influence on Baltic cod, sprat, and herring stock–recruitment relationships

    DEFF Research Database (Denmark)

    Margonski, Piotr; Hansson, Sture; Tomczak, Maciej

    2010-01-01

    is significantly related to spawning stock biomass, climatic forcing, and feeding conditions and (ii) by acknowledging these drivers, management decisions can be improved. Climate impact expressed by climatic indices or changes in water temperature was included in all the final models. Recruitment of the herring...

  19. The parasite Ichthyophonus sp. in Pacific herring from the coastal NE Pacific

    Science.gov (United States)

    Hershberger, Paul K.; Gregg, Jacob L.; Hart, Lucas M.; Moffitt, Steve; Brenner, Richard L.; Stick, K.; Coonradt, Eric; Otis, E. O.; Vollenweider, Johanna J.; Garver, Kyle A.; Lovy, Jan; Meyers, T.R.

    2016-01-01

    The protistan parasite Ichthyophonus occurred in populations of Pacific herring Clupea pallasii Valenciennes throughout coastal areas of the NE Pacific, ranging from Puget Sound, WA north to the Gulf of Alaska, AK. Infection prevalence in local Pacific herring stocks varied seasonally and annually, and a general pattern of increasing prevalence with host size and/or age persisted throughout the NE Pacific. An exception to this zoographic pattern occurred among a group of juvenile, age 1+ year Pacific herring from Cordova Harbor, AK in June 2010, which demonstrated an unusually high infection prevalence of 35%. Reasons for this anomaly were hypothesized to involve anthropogenic influences that resulted in locally elevated infection pressures. Interannual declines in infection prevalence from some populations (e.g. Lower Cook Inlet, AK; from 20–32% in 2007 to 0–3% during 2009–13) or from the largest size cohorts of other populations (e.g. Sitka Sound, AK; from 62.5% in 2007 to 19.6% in 2013) were likely a reflection of selective mortality among the infected cohorts. All available information for Ichthyophonus in the NE Pacific, including broad geographic range, low host specificity and presence in archived Pacific herring tissue samples dating to the 1980s, indicate a long-standing host–pathogen relationship.

  20. Feeding and growth of larval herring,Clupea harengus, in relation to density of copepod nauplii

    DEFF Research Database (Denmark)

    Kiørboe, Thomas; Munk, Peter

    1986-01-01

    Feeding and growth rates of 1–3 wk old herring larvae from four different stocks were compared in laboratory experiments (8°C). For most of the larval groups, feeding rate was saturated at nauplii (Acartia tonsa, nauplii stages 3–5) densities over 301−1 (5 μg d.w. 1−1). Specific growth rate incre...

  1. Organochlorine pesticides and polychlorinated biphenyls in herring from the southern Baltic, 1983

    Energy Technology Data Exchange (ETDEWEB)

    Falandysz, J

    1986-02-01

    Hexachlorobenzene (HCB), alpha-, beta-, gamma- and delta-benzenehexachloride (BHC, HCH), p,p'-DDE, o,p'-DDD, o,p'-DDT, p,p'-DDD and p,p'-DDT (sigma DDT) and polychlorinated biphenyls (PCB) levels have been determined in muscle tissue of 187 herring (Clupea harengus) netted during 1983 in a different regions in the southern part of the Baltic Sea. The mean levels found for herring muscle tissue related to wet weight (microgram/kg) were: 14 HCB, 18 alpha-BHC, 23 beta-BHC, 14 gamma-BHC, delta-BHC remained undetected, 56 sigma BHC, 115 p,p'-DDE, o,p'-DDD and o,p'-DDT remained undetected, 84 p,p'-DDD, 51 p,p'-DDT, 250 sigma DDT and 530 PCB. The levels of organochlorine pesticides determined in wet muscles or extractable lipids of herring are nearly 2-3 times as high as those noted in fish sampled in the same area in two years before, while for PCBs the wet weight levels were comparable, and when based on a lipid weight are somewhat higher. The results are compared with levels found in herring collected in different regions of the Baltic Sea during 1965-1983, and reported previously by other authors.

  2. Bayesian stock assessment of Pacific herring in Prince William Sound, Alaska.

    Directory of Open Access Journals (Sweden)

    Melissa L Muradian

    Full Text Available The Pacific herring (Clupea pallasii population in Prince William Sound, Alaska crashed in 1993 and has yet to recover, affecting food web dynamics in the Sound and impacting Alaskan communities. To help researchers design and implement the most effective monitoring, management, and recovery programs, a Bayesian assessment of Prince William Sound herring was developed by reformulating the current model used by the Alaska Department of Fish and Game. The Bayesian model estimated pre-fishery spawning biomass of herring age-3 and older in 2013 to be a median of 19,410 mt (95% credibility interval 12,150-31,740 mt, with a 54% probability that biomass in 2013 was below the management limit used to regulate fisheries in Prince William Sound. The main advantages of the Bayesian model are that it can more objectively weight different datasets and provide estimates of uncertainty for model parameters and outputs, unlike the weighted sum-of-squares used in the original model. In addition, the revised model could be used to manage herring stocks with a decision rule that considers both stock status and the uncertainty in stock status.

  3. Lumpers or splitters? Evaluating recovering and management plans for metapopulations of herring

    NARCIS (Netherlands)

    Kell, L.T.; Dickey-Collas, M.; Hintzen, N.T.; Nash, R.D.M.; Pilling, G.M.

    2009-01-01

    The long-term management of a stock representing a metapopulation has been simulated in a case study loosely based upon herring to the west of the British isles, where stocks are currently assessed and managed by management area, although there is evidence of mixing between stocks (in terms of

  4. Influence of ionizing radiation on the fatty acid composition of herring fillets

    International Nuclear Information System (INIS)

    Adam, S.; Paul, G.; Ehlermann, D.

    1982-01-01

    The effect of γ-irradiation (absorbed dose: 50 kGy, dose-rate: 2.9 kGy/h) on the distribution of fatty acid components in herring fillets has been examined using high-resolution gas chromatographic methods. Radiolytic treatment at 0 0 C and exclusion of atmospheric oxygen caused no significant decrease in the relative amounts of the constituent saturated, monounsaturated and polyunsaturated fatty acid components. Specifically, eicosapentaenoic acid (20:5) and docosahexaenoic acid (22:6), which are of particular physiological interest were not affected by γ-rays, even after additional storage of the irradiated material at 0 0 C for 4 weeks. Irradiation of oil extracted from herring fillets or of herring oil/water emulsions under aerobic conditions, however, destroyed eicosapentaenoic acid and docosahexaenoic acid significantly. The loss of radio-resistance - as compared to the radiation-induced processes in the fillets - is explained by the absence of proteins, which effectively protect the lipid components from radiolytic decomposition. It is concluded that the commercial radiation processing of herring at the recommended dose levels (1 to 2 kGy) should not reduce the content of unsaturated fatty acid components. (author)

  5. Histopathology of adult Pacific herring in Prince William Sound, Alaska, after the Exxon Valdez oil spill

    International Nuclear Information System (INIS)

    Marty, G. S.; Okihiro, M. S.; Hinton, D. E.; Brown, E. D.; Hanes, D.

    1999-01-01

    The histopathology of Pacific herring sampled from oiled sites in Prince William Sound, Alaska, three weeks after the 1989 Exxon Valdez oil spill, is discussed. All samples showed multifocal hepatic necrosis and significantly increased tissue concentrations of polynuclear aromatic hydrocarbons (PAH). In contrast, Pacific herring from reference sites in 1989 and from all sites in 1990 and 1991 did not have hepatic necrosis or increase PAH concentrations. Naphthalenes were the predominant PAH in all tissue samples. The development of hepatic necrosis and the predominance of naphthalenes in samples from 1989 is considered consistent with results obtained from recent laboratory studies indicating that crude oil exposure resulted in dose-dependent expression of viral hemorrhagic septicemia virus (VHSV). It was concluded that adult Pacific herring in Prince William Sound were likely exposed to Exxon Valdez oil in April 1989. Because Pacific herring near spawning condition in early spring are in a state of physiological stress, the added stress of oil exposure in 1989 could reasonably have led to expression of VHSV in these fish. The findings suggest that the immediate response to future large toxic spills should include consideration of the potential interaction of multiple stressors in exposed individuals. 29 refs., 7 tabs., 1 fig

  6. Use of herring bait to farm lobsters in the Gulf of Maine.

    Directory of Open Access Journals (Sweden)

    Jonathan H Grabowski

    2010-04-01

    Full Text Available Ecologists, fisheries scientists, and coastal managers have all called for an ecosystem approach to fisheries management, yet many species such as the American lobster (Homarus americanus are still largely managed individually. One hypothesis that has yet to be tested suggests that human augmentation of lobster diets via the use of Atlantic herring (Clupea harengus as bait may contribute to recent increases in lobster landings. Currently 70% of Atlantic herring landings in the Gulf of Maine are used as bait to catch lobsters in traps throughout coastal New England.We examined the effects of this herring bait on the diet composition and growth rate of lobsters at heavily baited vs. seasonally closed (i.e., bait free sites in coastal Maine. Our results suggest that human use of herring bait may be subsidizing juvenile lobster diets, thereby enhancing lobster growth and the overall economic value and yield of one of the most valuable fisheries in the U.S.Our study illustrates that shifting to an ecosystem approach to fisheries management should require consideration of cross-fishery interactions.

  7. The parasite Ichthyophonus sp. in Pacific herring from the coastal NE Pacific.

    Science.gov (United States)

    Hershberger, P K; Gregg, J L; Hart, L M; Moffitt, S; Brenner, R; Stick, K; Coonradt, E; Otis, E O; Vollenweider, J J; Garver, K A; Lovy, J; Meyers, T R

    2016-04-01

    The protistan parasite Ichthyophonus occurred in populations of Pacific herring Clupea pallasii Valenciennes throughout coastal areas of the NE Pacific, ranging from Puget Sound, WA north to the Gulf of Alaska, AK. Infection prevalence in local Pacific herring stocks varied seasonally and annually, and a general pattern of increasing prevalence with host size and/or age persisted throughout the NE Pacific. An exception to this zoographic pattern occurred among a group of juvenile, age 1+ year Pacific herring from Cordova Harbor, AK in June 2010, which demonstrated an unusually high infection prevalence of 35%. Reasons for this anomaly were hypothesized to involve anthropogenic influences that resulted in locally elevated infection pressures. Interannual declines in infection prevalence from some populations (e.g. Lower Cook Inlet, AK; from 20-32% in 2007 to 0-3% during 2009-13) or from the largest size cohorts of other populations (e.g. Sitka Sound, AK; from 62.5% in 2007 to 19.6% in 2013) were likely a reflection of selective mortality among the infected cohorts. All available information for Ichthyophonus in the NE Pacific, including broad geographic range, low host specificity and presence in archived Pacific herring tissue samples dating to the 1980s, indicate a long-standing host-pathogen relationship. © 2015 John Wiley & Sons Ltd.

  8. Quality of crude fish oil extracted from herring byproducts of varying states of freshness

    NARCIS (Netherlands)

    Aidos, I.M.; Padt, van der A.; Boom, R.M.; Luten, J.B.

    2003-01-01

    Herring byproducts were stored at 2 and 15degreesC for up to 72 h. Over time, significant increases of total volatile bases (TVB), histamine, putrescine, cadaverine, and tyramine were detected. However, only tyramine and TVB levels were temperature-dependent. The level of total polyunsaturated

  9. Realized habitats of early-stage North Sea herring: looking for signals of environmental change

    DEFF Research Database (Denmark)

    Röckmann, C.; Dickey-Collas, M.; Payne, Mark

    2011-01-01

    Realized habitats of North Sea herring for two larval and two juvenile stages were estimated over the past 30 years, using abundances from surveys tied to modelled estimates of temperature and salinity. Newly hatched larvae (NHL) were found mainly in water masses of 9–11°C, pre-metamorphosis larvae...

  10. Dans l’atelier de l’écrivain (autofictif. L’enterrement de la sardine de Patrice Lessard

    Directory of Open Access Journals (Sweden)

    Marilyn Randall

    2017-03-01

    Full Text Available L’enterrement de la sardine, dernier roman de la trilogie lisboète de Patrice Lessard, nous introduit à la fois dans le labyrinthe de la vieille ville de Lisbonne et dans celui de la narration de deux romans en train de se faire. La distinction entre fiction et autobiographie proposée dans l’avant-propos s’effondre vite devant les multiples intrusions du « réel » dans la fiction et de la fiction dans le « réel ». De plus, la facture fragmentaire de l’ensemble pose un défi au lecteur qui se trouve en plein atelier de l’écrivain, devant un portrait de l’artiste inachevé, inachevable. L’enterrement de la sardine, the final volume of Patrick Lessard’s Lisbonne trilogy, plunges the reader into the labyrinth of Lisbonne’s old city as well as into the meanders of two novels in the making. The distinction between fiction and autobiography presented in the preface quickly dissolves in the face of the multiple intrusions of the “real” world into the fictional one and vice versa. The fragmentary nature of the entire text poses a challenge to the reader, who seems to have wandered into the writer’s workshop to confront an unfinished — perhaps unfinishable — self-portrait.

  11. Optimization of α-tocopherol and ascorbyl palmitate addition for the stabilization of sardine oil

    Directory of Open Access Journals (Sweden)

    Morales-Medina, R.

    2015-06-01

    Full Text Available The purpose of the present work was to optimize the addition of natural antioxidants (α- tocopherol and ascorbyl palmitate for the stabilization of sardine oil rich in omega-3 PUFA. The optimal values for peroxide value (PV, which minimizes primary oxidation products, were obtained at low concentrations of α-tocopherol (50–207 ppm, high content of ascorbyl palmitate (450 ppm and 50 ppm citric acid. On the other hand, optimal values for p-anisidine value (AV, which minimizes secondary oxidation products, were found at medium concentrations of α-tocopherol (478–493 ppm, high contents of ascorbyl palmitate (390–450 ppm and 50 ppm citric acid. The conflicting effect of α-tocopherol on the individual optimization of PV and AV motivated the generation of a Pareto front (set of non inferior solutions employing the weighted-sum multi-objective optimization technique.El objetivo de este trabajo fue optimizar la adición de antioxidantes naturales (α-tocoferol y palmitato de ascorbilo para la estabilización de aceite de sardina rico en omega-3 PUFA. Bajas concentraciones de α-tocoferol (50–207 ppm combinadas con la adicción de antioxidantes secundarios como palmitato de ascorbilo (450 ppm y ácido cítrico (50 ppm, minimizaron la formación de hidroperóxidos en el aceite de sardina estudiado. Sin embargo, los productos secundarios de oxidación se redujeron para concentraciones medias de α-tocoferol (478–493 ppm, altas de palmitato de ascorbilo (390–450 ppm y 50 ppm de ácido cítrico. El efecto contradictorio de la concentración de α-tocoferol en la optimización individual del índice de peróxidos e índice de p-anisidina motivó la realización de una optimización simultánea que permite satisfacer la optimización de cada una de las variables individuales en el grado deseado.

  12. Linking sardine recruitment in coastal areas to ocean currents using surface drifters and HF radar. A case study in the Gulf of Manfredonia, Adriatic Sea

    DEFF Research Database (Denmark)

    Sciascia, Roberta; Berta, Maristella; Carlson, Daniel Frazier

    2017-01-01

    Understanding the role of ocean currents in the recruitment of commercially and ecologically important fish is an important step towards developing sustainable resource management guidelines. To this end, we attempt to elucidate the role of surface ocean transport in supplying recruits of sardine...

  13. Feeding behaviour and growth of the Peruvian scallop (Argopecten purpuratus) under daily cyclic hypoxia conditions

    Science.gov (United States)

    Aguirre-Velarde, Arturo; Jean, Fred; Thouzeau, Gérard; Flye-Sainte-Marie, Jonathan

    2018-01-01

    As a secondary consequence of the high productivity of the upwelling system, organisms inhabiting Peruvian coastal bays are frequently exposed to hypoxic conditions. The aim of the present paper was to investigate the effects of daily-cyclic-severe hypoxia on energetics of a species presenting little escape ability when facing hypoxia. For this purpose, juvenile Peruvian scallops (Argopecten purpuratus) were exposed to four experimental conditions: fed and starved, combined or not to nightly severe hypoxia (5% oxygen saturation) for ≈ 12 h over a 21-day experiment. In both fed conditions, clearance rate was measured by the mean of an open-flow system. Our results indicate that the Peruvian scallop is able to maintain an active filtration even at low oxygen saturation, at least during expositions up to 12 h. During the first phase of exposure to hypoxia, clearance rate decreased abruptly when oxygen saturation dropped below 10%, but rapidly recovered to values close to those found under normoxia. As a consequence of this ability to feed during hypoxia, no difference in soft tissue dry weight (digestive gland not included) was observed at the end of the experimental period between oxic conditions among fed scallops. However, shell growth was negatively affected by hypoxic condition. Starved individuals exhibited similar weight loss between hypoxic and normoxic conditions indicating no or little effect of oxic condition on maintenance costs. Considering the observed responses for feeding, growth and maintenance, we can hypothesize that this species presents metabolic/bioenergetic efficient adaptations to deal with hypoxic conditions that are recurrent in Peruvian coastal bays. We hypothesize that the small observed effects might be modelled in the context of the Dynamic Energy Budget theory as a restriction of reserve mobilization under hypoxic conditions.

  14. Biomineralization changes with food supply confer juvenile scallops (Argopecten purpuratus) resistance to ocean acidification

    KAUST Repository

    Ramajo, Laura; Marbà , Nú ria; Prado, Luis; Peron, Sophie; Lardies, Marco A.; Rodriguez-Navarro, Alejandro; Vargas, Cristian A.; Lagos, Nelson A.; Duarte, Carlos M.

    2015-01-01

    Future ocean acidification (OA) will affect physiological traits of marine species, with calcifying species being particularly vulnerable. As OA entails high energy demands, particularly during the rapid juvenile growth phase, food supply may play a key role in the response of marine organisms to OA. We experimentally evaluated the role of food supply in modulating physiological responses and biomineralization processes in juveniles of the Chilean scallop, Argopecten purpuratus, that were exposed to control (pH ~ 8.0) and low pH (pH ~ 7.6) conditions using three food supply treatments (high, intermediate, and low). We found that pH and food levels had additive effects on the physiological response of the juvenile scallops. Metabolic rates, shell growth, net calcification, and ingestion rates increased significantly at low pH conditions, independent of food. These physiological responses increased significantly in organisms exposed to intermediate and high levels of food supply. Hence, food supply seems to play a major role modulating organismal response by providing the energetic means to bolster the physiological response of OA stress. On the contrary, the relative expression of chitin synthase, a functional molecule for biomineralization, increased significantly in scallops exposed to low food supply and low pH, which resulted in a thicker periostracum enriched with chitin polysaccharides. Under reduced food and low pH conditions, the adaptive organismal response was to trade-off growth for the expression of biomineralization molecules and altering of the organic composition of shell periostracum, suggesting that the future performance of these calcifiers will depend on the trajectories of both OA and food supply. Thus, incorporating a suite of traits and multiple stressors in future studies of the adaptive organismal response may provide key insights on OA impacts on marine calcifiers.

  15. Biomineralization changes with food supply confer juvenile scallops (Argopecten purpuratus) resistance to ocean acidification

    KAUST Repository

    Ramajo, Laura

    2015-12-08

    Future ocean acidification (OA) will affect physiological traits of marine species, with calcifying species being particularly vulnerable. As OA entails high energy demands, particularly during the rapid juvenile growth phase, food supply may play a key role in the response of marine organisms to OA. We experimentally evaluated the role of food supply in modulating physiological responses and biomineralization processes in juveniles of the Chilean scallop, Argopecten purpuratus, that were exposed to control (pH ~ 8.0) and low pH (pH ~ 7.6) conditions using three food supply treatments (high, intermediate, and low). We found that pH and food levels had additive effects on the physiological response of the juvenile scallops. Metabolic rates, shell growth, net calcification, and ingestion rates increased significantly at low pH conditions, independent of food. These physiological responses increased significantly in organisms exposed to intermediate and high levels of food supply. Hence, food supply seems to play a major role modulating organismal response by providing the energetic means to bolster the physiological response of OA stress. On the contrary, the relative expression of chitin synthase, a functional molecule for biomineralization, increased significantly in scallops exposed to low food supply and low pH, which resulted in a thicker periostracum enriched with chitin polysaccharides. Under reduced food and low pH conditions, the adaptive organismal response was to trade-off growth for the expression of biomineralization molecules and altering of the organic composition of shell periostracum, suggesting that the future performance of these calcifiers will depend on the trajectories of both OA and food supply. Thus, incorporating a suite of traits and multiple stressors in future studies of the adaptive organismal response may provide key insights on OA impacts on marine calcifiers.

  16. Balancing the edge effects budget: bay scallop settlement and loss along a seagrass edge.

    Science.gov (United States)

    Carroll, John M; Furman, Bradley T; Tettelbach, Stephen T; Peterson, Bradley J

    2012-07-01

    Edge effects are a dominant subject in landscape ecology literature, yet they are highly variable and poorly understood. Often, the literature suggests simple models for edge effects-positive (enhancement at the edge), negative (enhancement at the interior), or no effect (neutral)--on a variety of metrics, including abundance, diversity, and mortality. In the marine realm, much of this work has focused on fragmented seagrass habitats due to their importance for a variety of commercially important species. In this study, the settlement, recruitment, and survival of bay scallops was investigated across a variety of seagrass patch treatments. By simultaneously collecting settlers (those viable larvae available to settle and metamorphose) and recruits (those settlers that survive some period of time, in this case, 6 weeks) on the same collectors, we were able to demonstrate a "balance" between positive and negative edge effects, resulting in a net neutral effect. Scallop settlement was significantly enhanced along seagrass edges, regardless of patch type while survival was elevated within patch interiors. However, recruitment (the net result of settlement and post-settlement loss) did not vary significantly from edge to center, representing a neutral effect. Further, results suggest that post-settlement loss, most likely due to predation, appears to be the dominant mechanism structuring scallop abundance, not patterns in settlement. These data illustrate the complexity of edge effects, and suggest that the metric used to investigate the effect (be it abundance, survival, or other metrics) can often influence the magnitude and direction of the perceived effect. Traditionally, high predation along a habitat edge would have indicated an "ecological trap" for the species in question; however, this study demonstrates that, at the population level, an ecological trap may not exist.

  17. Spatial changes in fatty acids signatures of the great scallop Pecten maximus across the Bay of Biscay continental shelf

    Science.gov (United States)

    Nerot, Caroline; Meziane, Tarik; Schaal, Gauthier; Grall, Jacques; Lorrain, Anne; Paulet, Yves-Marie; Kraffe, Edouard

    2015-10-01

    The spatial variability of food resources along continental margins can strongly influence the physiology and ecology of benthic bivalves. We explored the variability of food sources of the great scallop Pecten maximus, by determining their fatty acid (FA) composition along an inshore-offshore gradient in the Bay of Biscay (from 15 to 190 m depth). The FA composition of the digestive gland showed strong differences between shallow and deep-water habitats. This trend was mainly driven by their content in diatom-characteristic fatty acids, which are abundant near the coast. Scallops collected from the middle of the continental shelf were characterized by higher contents of flagellate markers than scallops from shallow habitats. This could be related to a permanent vertical stratification in the water column, which reduced vertical mixing of waters, thereby enhancing organic matter recycling through the microbial loop. In the deeper water station (190 m), FA compositions were close to the compositions found in scallops from shallow areas, which suggest that scallops could have access to the same resources (i.e. diatoms). Muscle FA composition was more indicative of the physiological state of scallops over this depth range, revealing contrasting reproductive strategies among the two coastal sites and metabolic or physiological adaptation at greater depth (e.g. structural and functional adjustments of membrane composition). This study therefore revealed contrasted patterns between shallow and deeper habitats for both P. maximus muscle and digestive gland tissues. This emphasizes the variability in the diet of this species along its distribution range, and stresses the importance of analyzing different tissues for their FA composition in order to better understand their physiology and ecology.

  18. A dopa decarboxylase modulating the immune response of scallop Chlamys farreri.

    Directory of Open Access Journals (Sweden)

    Zhi Zhou

    Full Text Available BACKGROUND: Dopa decarboxylase (DDC is a pyridoxal 5-phosphate (PLP-dependent enzyme that catalyzes the decarboxylation of L-Dopa to dopamine, and involved in complex neuroendocrine-immune regulatory network. The function for DDC in the immunomodulation remains unclear in invertebrate. METHODOLOGY: The full-length cDNA encoding DDC (designated CfDDC was cloned from mollusc scallop Chlamys farreri. It contained an open reading frame encoding a polypeptide of 560 amino acids. The CfDDC mRNA transcripts could be detected in all the tested tissues, including the immune tissues haemocytes and hepatopancreas. After scallops were treated with LPS stimulation, the mRNA expression level of CfDDC in haemocytes increased significantly (5.5-fold, P<0.05 at 3 h and reached the peak at 12 h (9.8-fold, P<0.05, and then recovered to the baseline level. The recombinant protein of CfDDC (rCfDDC was expressed in Escherichia coli BL21 (DE3-Transetta, and 1 mg rCfDDC could catalyze the production of 1.651±0.22 ng dopamine within 1 h in vitro. When the haemocytes were incubated with rCfDDC-coated agarose beads, the haemocyte encapsulation to the beads was increased significantly from 70% at 6 h to 93% at 24 h in vitro in comparison with that in the control (23% at 6 h to 25% at 24 h, and the increased haemocyte encapsulation was repressed by the addition of rCfDDC antibody (which is acquired via immunization 6-week old rats with rCfDDC. After the injection of DDC inhibitor methyldopa, the ROS level in haemocytes of scallops was decreased significantly to 0.41-fold (P<0.05 of blank group at 12 h and 0.47-fold (P<0.05 at 24 h, respectively. CONCLUSIONS: These results collectively suggested that CfDDC, as a homologue of DDC in scallop, modulated the immune responses such as haemocytes encapsulation as well as the ROS level through its catalytic activity, functioning as an indispensable immunomodulating enzyme in the neuroendocrine-immune regulatory network of mollusc.

  19. An analysis of the potential for oil spill effects on the herring population of Prince William Sound, Alaska

    International Nuclear Information System (INIS)

    Pearson, W.H.; Elston, R.A.; Bienert, R.W.; Drum, A.S.; Antrim, L.D.

    1996-01-01

    The impact of the Exxon Valdez oil spill on the herring population in Prince William Sound, was studied. Following the incident in 1989, there was no commercial harvest of herring. However, 1990 through 1992 proved to have above average, record harvests. This was followed by a dramatic decline in 1993 which was generally attributed to the oil spill. An examination of the scientific data was conducted. The main hypotheses for the decline were tested. These hypotheses attributed the decline to: (1) the oil spill itself, (2) a combination of increasing herring biomass and decreasing food supply, (3) disease, and (4) other natural stochastic processes. Based on the review of the data and the analysis of the four alternative hypotheses, it was concluded that the population decline of 1993 was the result of a combination of increasing herring biomass and decreasing food supply. No connection to the Exxon Valdez incident was evident. 22 refs., 6 figs

  20. Economic gains from introducing international ITQs - the case of the mackerel and herring fisheries in the Northeast Atlantic

    DEFF Research Database (Denmark)

    Thøgersen, Thomas Talund; Eigaard, Ole Ritzau; Fitzpatrick, M.

    2015-01-01

    Achieving a balance between fishing capacity and fishing opportunities is one of the major challenges in European fisheries. One way to achieve this is to introduce individual tradable quotas or similar management measures. In several mackerel and herring fisheries in the Northeast Atlantic...... that the gross cash flow can be improved by 21% by allowing the mackerel and herring quotas to be traded internationally in the Northeast Atlantic. This rent gain arises mainly from increased productivity by allowing tradability between areas and fleets. The analysis also shows that the Danish pelagic fleet...... will gain from increasing its share of mackerel and herring quotas, whereas the Irish fleets are incentivised to sell quota, if individual quotas are allowed to be traded among countries. This result is in line with the qualitative analyses that show that Irish fishermen targeting herring in the Celtic Sea...

  1. Forage fish quality: seasonal lipid dynamics of herring (Clupea harengus L.) and sprat (Sprattus sprattus L.) in the Baltic Sea

    DEFF Research Database (Denmark)

    Røjbek, Maria; Tomkiewicz, Jonna; Jacobsen, Charlotte

    2013-01-01

    seasonally with high levelstowards the end of the annual zooplankton production cycle, succeeded by a decline. Lipid content and fatty acid composition differed significantly between sprat and herring. Sprat lipid content was higher than herring, increasing with fish size and characterized by large......This study investigates lipid content and fatty acid composition of two important forage fish, sprat (Sprattus sprattus) and herring (Clupea harengus) in the Baltic Sea ecosystem. Seasonal variation in lipids was studied during three periods following the annual reproductive cycle considering...... potential differences relating to fish size, sex, and reproductive status. The isopod Saduria entomon, being at times an important prey for predatory fish, was included for comparison. In both sprat and herring, lipid content and absolute contents of essential polyunsaturated fatty acids (PUFAs) varied...

  2. Quantification of biomolecules in herring (Clupea harengus) industry processing waters and their recovery using electroflocculation and ultrafiltration

    DEFF Research Database (Denmark)

    Osman, Ali; Gringer, Nina; Svendsen, Tore

    2015-01-01

    Four types of herring industry processing waters; refrigerated sea water (RSW), storage water (SW), processing water from cutting (PW) and pre-salting brines (SB) were subjected to chemical characterization and biomolecule recovery using electroflocculation (EF) and ultrafiltration (UF). The high......Four types of herring industry processing waters; refrigerated sea water (RSW), storage water (SW), processing water from cutting (PW) and pre-salting brines (SB) were subjected to chemical characterization and biomolecule recovery using electroflocculation (EF) and ultrafiltration (UF...

  3. Sardine (sardina Pilchardus) Larval Dispersal in Northern Canary Current Upwelling System (iberian Peninsula), Using Coupled Biophysical Techniques

    Science.gov (United States)

    Santos, A. M. P. A.; Nieblas, A. E.; Verley, P.; Teles-Machado, A.; Bonhommeau, S.; Lett, C.; Garrido, S.; Peliz, A.

    2017-12-01

    The European sardine (Sardina pilchardus) is the most important small pelagic fishery of the Western Iberia Upwelling Ecosystem (WIUE). Recently, recruitment of this species has declined due to changing environmental conditions. Furthermore, controversies exist regarding its population structure with barriers thought to exist between the Atlantic-Iberian Peninsula, Northern Africa, and the Mediterranean. Few studies have investigated the transport and dispersal of sardine eggs and larvae off Iberia and the subsequent impact on larval recruitment variability. Here, we examine these issues using a Regional Ocean Modeling System climatology (1989-2008) coupled to the Lagrangian transport model, Ichthyop. Using biological parameters from the literature, we conduct simulations that investigate the effects of spawning patchiness, diel vertical migration behaviors, and egg buoyancy on the transport and recruitment of virtual sardine ichthyoplankton on the continental shelf. We find that release area, release depth, and month of release all significantly affect recruitment. Patchiness has no effect and diel vertical migration causes slightly lower recruitment. Egg buoyancy effects are significant and act similarly to depth of release. As with other studies, we find that recruitment peaks vary by latitude, explained here by the seasonal variability of offshore transport. We find weak, continuous alongshore transport between release areas, though a large proportion of simulated ichthyoplankton transport north to the Cantabrian coast (up to 27%). We also show low level transport into Morocco (up to 1%) and the Mediterranean (up to 8%). The high proportion of local retention and low but consistent alongshore transport supports the idea of a series of metapopulations along this coast. This study was supported by the Portuguese Science and Technology Foundation (FCT) through the research project MODELA (PTDC/MAR/098643/2008) and MedEx (MARIN-ERA/MAR/0002/2008). MedEx is also a

  4. Cholinesterase activities in the scallop Pecten jacobaeus: Characterization and effects of exposure to aquatic contaminants

    International Nuclear Information System (INIS)

    Stefano, Bonacci; Ilaria, Corsi; Silvano, Focardi

    2008-01-01

    Nearshore marine environments of industrialized countries are increasingly threatened by anthropogenic pollution. It is therefore a priority task to investigate the sensitivity of new ecotoxicological warning signals of the occurrence and effects of aquatic pollutants. The main aims of the present study were: 1) to characterize the biochemical properties of ChEs in tissues of the bivalve Pecten jacobaeus, using different specific substrates and selective inhibitors; 2) to measure sensitivity of ChE activities to in vitro exposure to the OPs azamethiphos and DFP and to the heavy metals cadmium and zinc. Our final aim was to carry out a preliminary evaluation of the suitability of ChEs measurement in tissues of the scallop for monitoring marine environmental quality and neurotoxic compounds contamination in the Mediterranean Sea. Responses to specific inhibitors have suggested that ChEs in adductor muscle share many characteristics with vertebrate acetylcholinesterase. Dose-dependent inhibition of ChE was observed in response to in vitro exposure to environmental contaminants such as cadmium and azamethiphos. Sensitivity to zinc and DFP was lower. ChEs in P. jacobaeus might therefore have potential as a sensitive biomarker for monitoring marine pollution. Results of the present study will be useful to focus further experiment of exposure to pollutants under in vivo conditions. Capsule: Cholinesterase activities in scallop Pecten jacobaeus were observed to be sensitive to contaminants in vitro and may therefore have potential as biomarkers for monitoring water pollution

  5. Cholinesterase activities in the scallop Pecten jacobaeus: Characterization and effects of exposure to aquatic contaminants

    Energy Technology Data Exchange (ETDEWEB)

    Stefano, Bonacci [Department of Environmental Sciences ' G. Sarfatti' , University of Siena, Via P.A. Mattioli 4, I-53100 Siena (Italy)], E-mail: bonacci@unisi.it; Ilaria, Corsi [Department of Environmental Sciences ' G. Sarfatti' , University of Siena, Via P.A. Mattioli 4, I-53100 Siena (Italy)], E-mail: corsii@unisi.it; Silvano, Focardi [Department of Environmental Sciences ' G. Sarfatti' , University of Siena, Via P.A. Mattioli 4, I-53100 Siena (Italy)], E-mail: focardi@unisi.it

    2008-03-15

    Nearshore marine environments of industrialized countries are increasingly threatened by anthropogenic pollution. It is therefore a priority task to investigate the sensitivity of new ecotoxicological warning signals of the occurrence and effects of aquatic pollutants. The main aims of the present study were: 1) to characterize the biochemical properties of ChEs in tissues of the bivalve Pecten jacobaeus, using different specific substrates and selective inhibitors; 2) to measure sensitivity of ChE activities to in vitro exposure to the OPs azamethiphos and DFP and to the heavy metals cadmium and zinc. Our final aim was to carry out a preliminary evaluation of the suitability of ChEs measurement in tissues of the scallop for monitoring marine environmental quality and neurotoxic compounds contamination in the Mediterranean Sea. Responses to specific inhibitors have suggested that ChEs in adductor muscle share many characteristics with vertebrate acetylcholinesterase. Dose-dependent inhibition of ChE was observed in response to in vitro exposure to environmental contaminants such as cadmium and azamethiphos. Sensitivity to zinc and DFP was lower. ChEs in P. jacobaeus might therefore have potential as a sensitive biomarker for monitoring marine pollution. Results of the present study will be useful to focus further experiment of exposure to pollutants under in vivo conditions. Capsule: Cholinesterase activities in scallop Pecten jacobaeus were observed to be sensitive to contaminants in vitro and may therefore have potential as biomarkers for monitoring water pollution.

  6. Effect of the Algaecide Palmitoleic Acid on the Immune Function of the Bay Scallop Argopecten irradians

    Directory of Open Access Journals (Sweden)

    Cheng Chi

    2016-05-01

    Full Text Available Palmitoleic acid (PA, an algicidal compound, is used against the toxin producing dinofagelate Alexandrium tamarense, however, its impact on the edible bay scallop (Argopecten irradians is still unclear. Therefore, we investigated the impacts of effective algicidal concentrations (20, 40, and 80 mg/L of PA on immune responses in A. irradians. Various immune parameters including acid phosphatase (ACP activity, superoxide dismutase (SOD, lysozyme, phagocytic activity, total protein, malondialdehyde (MDA level, and reactive oxygen species (ROS production and the expression of immune-related genes (PrxV, CLT-6, MT, and BD were measured at 3, 6, 12, 24, and 48 h post-exposure (hpe to PA. Lysozyme activity was lower in scallops at 12–48 hpe to 80 mg/L. SOD, ACP activity, ROS production, the total protein, and MDA level was higher at 12 to 48 hpe with different concentrations of PA. Phagocytic activity increased at 6–12 hpe to 40–80 mg/L of PA, but decreased at 24–48 hpe. The expressions of genes PrxV, CLT-6, MT and BD down-regulated at 3 hpe were observed, while differential expressions from 6–48 hpe with different concentrations of PA. The present study demonstrated that immersing A. irradians in PA at effective concentrations could result in differential effects on non-specific immune responses and expressions of immune-related genes.

  7. Oxidative and interactive challenge of cadmium and ocean acidification on the smooth scallop Flexopecten glaber.

    Science.gov (United States)

    Nardi, Alessandro; Benedetti, Maura; Fattorini, Daniele; Regoli, Francesco

    2018-03-01

    Ocean acidification (OA) may affect sensitivity of marine organisms to metal pollution modulating chemical bioavailability, bioaccumulation and biological responsiveness of several cellular pathways. In this study, the smooth scallop Flexopecten glaber was exposed to various combinations of reduced pH (pH/pCO 2 7.4/∼3000 μatm) and Cd (20 μg/L). The analyses on cadmium uptake were integrated with those of a wide battery of biomarkers including metallothioneins, single antioxidant defenses and total oxyradical scavenging capacity in digestive gland and gills, lysosomal membrane stability and onset of genotoxic damage in haemocytes. Reduced pH slightly increased concentration of Cd in scallop tissues, but no effects were measured in terms of metallothioneins. Induction of some antioxidants by Cd and/or low pH in the digestive gland was not reflected in variations of the total oxyradical scavenging capacity, while the investigated stressors caused a certain inhibition of antioxidants and reduction of the scavenging capacity toward peroxyl radical in the gills. Lysosomal membrane stability and onset of genotoxic damages showed high sensitivity with possible synergistic effects of the investigated factors. The overall results suggest that indirect effects of ocean acidification on metal accumulation and toxicity are tissue-specific and modulate oxidative balance through different mechanisms. Copyright © 2018 Elsevier B.V. All rights reserved.

  8. Accumulation, biotransformation, histopathology and paralysis in the Pacific calico scallop Argopecten ventricosus by the paralyzing toxins of the dinoflagellate Gymnodinium catenatum.

    Science.gov (United States)

    Escobedo-Lozano, Amada Y; Estrada, Norma; Ascencio, Felipe; Contreras, Gerardo; Alonso-Rodriguez, Rosalba

    2012-05-01

    The dinoflagellate Gymnodinium catenatum produces paralyzing shellfish poisons that are consumed and accumulated by bivalves. We performed short-term feeding experiments to examine ingestion, accumulation, biotransformation, histopathology, and paralysis in the juvenile Pacific calico scallop Argopecten ventricosus that consume this dinoflagellate. Depletion of algal cells was measured in closed systems. Histopathological preparations were microscopically analyzed. Paralysis was observed and the time of recovery recorded. Accumulation and possible biotransformation of toxins were measured by HPLC analysis. Feeding activity in treated scallops showed that scallops produced pseudofeces, ingestion rates decreased at 8 h; approximately 60% of the scallops were paralyzed and melanin production and hemocyte aggregation were observed in several tissues at 15 h. HPLC analysis showed that the only toxins present in the dinoflagellates and scallops were the N-sulfo-carbamoyl toxins (C1, C2); after hydrolysis, the carbamate toxins (epimers GTX2/3) were present. C1 and C2 toxins were most common in the mantle, followed by the digestive gland and stomach-complex, adductor muscle, kidney and rectum group, and finally, gills. Toxin profiles in scallop tissue were similar to the dinoflagellate; biotransformations were not present in the scallops in this short-term feeding experiment.

  9. Hypolipidemic, antioxidant and antiatherogenic property of sardine by-products proteins in high-fat diet induced obese rats.

    Science.gov (United States)

    Affane, Fouad; Louala, Sabrine; El Imane Harrat, Nour; Bensalah, Fatima; Chekkal, Hadjera; Allaoui, Amine; Lamri-Senhadji, Myriem

    2018-04-15

    Fish by-products valorization on account of their richness in bioactive compounds may represent a better alternative to marine products with a view to economic profitability and sustainable development. In this study, we compared the effect of sardine by-product proteins (SBy-P), with those of the fillets (SF-P) or casein (Cas), on growth parameters, serum leptin level, lipids disorders, lipid peroxidation and reverse cholesterol transport, in diet-induced obese rats. Obesity was induced by feeding rats a high-fat diet (20% sheep fat), during 12 weeks. At body weight (BW) of 400 ± 20 g, eighteen obese rats were divided into three homogenous groups and continue to consume the high-fat diet for 4 weeks containing either, 20% SBy-P, SF-P or Cas. The results showed that SBy-P, compared to SF-P and Cas, efficiently reduced food intake (FI), BW gain and serum leptin level, and improved blood lipids levels and reverse cholesterol transport by reducing total cholesterol (TC), triacylglycerols (TG) and low-density lipoprotein cholesterol (LDL-HDL 1 -C) serum levels, increasing the level of high-density lipoprotein cholesterol (HDL 2 -C and HDL 3 -C), and enhancing lecithin: cholesterol acyltransferase (LCAT) activity. Furthermore, they attenuated lipid peroxidation by increasing atheroprotective activity of the paraoxonase-1 (PON-1). Sardine by-product proteins due to their richness in certain essential amino acids, highlight weight-loss, lipid-lowering, antioxidant and anti-atherogenic potentials, contributing to the improvement of the complications associated with obesity. Copyright © 2018 Elsevier Inc. All rights reserved.

  10. DNA damage in the gill cells of the marine scallop Mizuhopecten yessoensis during anoxic stress and aerobic recovery

    Science.gov (United States)

    Slobodskova, Valentina V.; Zhukovskaya, Avianna F.; Chelomin, Victor P.

    2012-06-01

    Anoxia-induced DNA damage in the gill cells of the marine scallop Mizuhopecten yessoensis was assessed with the alkaline comet assay (single-cell gel electrophoresis). The alkaline comet assay method for detecting DNA strand breaks and alkali labile sites in individual cells. DNA damage was determened in the scallops ( M. yessoensis) gill cells. The scallops were exposed to air for 8 h showing a clear increase in the levels of DNA damage. After the air exposure, M. yessoensis were re-submersed for a period of 12 h, leading values to return to a pre-aerial exposure level. Control animals were kept immersed during the whole period. The resulting data demonstrate that natural influences, such as oxygen depletion (anoxia) in seawater, can be responsible for the induction of DNA damage. If the scallops were re-immersed in oxic conditions, the anoxically induced breaks were repaired. The main mechanisms influencing the integrity of the DNA structure are discussed in this paper.

  11. Gene-associated markers can assign origin in a weakly structured fish, Atlantic herring

    DEFF Research Database (Denmark)

    Bekkevold, Dorte; Helyar, Sarah J.; Limborg, Morten T.

    2015-01-01

    , there are relatively few examples in marine fish displaying accurate origin assignment. We synthesize data for 156 single-nucleotide polymorphisms typed in 1039 herring, Clupea harengus L., spanning the Northeast Atlantic to develop a tool that allows assignment of individual herring to their regional origin. We show......Regulations on the exploitation of populations of commercially important fish species and the ensuing consumer interest in sustainable products have increased the need to accurately identify the population of origin of fish and fish products. Although genomics-based tools have proven highly useful...... the method's suitability to address specific biological questions, as well as management applications. We analyse temporally replicated collections from two areas, the Skagerrak (n = 81, 84, 66) and the western Baltic (n = 52, 52). Both areas harbour heavily fished mixed-origin stocks, complicating...

  12. Recruitment in a changing environment: the 2000s North Sea herring recruitment failure

    DEFF Research Database (Denmark)

    Payne, Mark; Hatfield, E.M.C.; Dickey-Collas, M.

    2009-01-01

    Environmentally induced change appears to be impacting the recruitment of North Sea herring (Clupea harengus). Despite simultaneously having a large adult population, historically low exploitation, and Marine Stewardship Council accreditation (implying sustainability), there have been an unpreced......Environmentally induced change appears to be impacting the recruitment of North Sea herring (Clupea harengus). Despite simultaneously having a large adult population, historically low exploitation, and Marine Stewardship Council accreditation (implying sustainability), there have been...... an unprecedented 6 sequential years of poor juvenile production (recruitment). Analysis suggests that the poor recruitment arises during the larval overwintering phase, with recent survival rates greatly reduced. Contemporary warming of the North Sea has caused significant changes in the plankton community...

  13. Foraging behaviour and prey size spectra of larval herring Clupea harengus

    DEFF Research Database (Denmark)

    Munk, Peter

    1992-01-01

    size groups of larval herring Clupea harengus L. were studied when preying on 6 size groups of copepods. Larval swimming and attack behaviour changed with prey size and were related to the ratio between prey length and larval length. The effective search rate showed a maximum when prey length was about......, that the available biomass of food as a proportion of the predator biomass will not increase. In order to assess the uniformity of relative prey size spectra of herring larvae and their background in larval foraging behaviour, a set of experimental and field investigations has been carried out. In the experiments, 4...... in the biomass spectra of the environment is important to larval growth and survival....

  14. Methods for conducting bioassays using embryos and larvae of Pacific herring, Clupea pallasi.

    Science.gov (United States)

    Dinnel, Paul A; Middaugh, Douglas P; Schwarck, Nathan T; Farren, Heather M; Haley, Richard K; Hoover, Richard A; Elphick, James; Tobiason, Karen; Marshall, Randall R

    2011-02-01

    The rapid decrease of several stocks of Pacific herring, Clupea pallasi, in Puget Sound, Washington, has led to concerns about the effects of industrial and nonpoint source contamination on the embryo and larval stages of this and related forage fish species. To address these concerns, the state of Washington and several industries have funded efforts to develop embryo and larval bioassay protocols that can be used by commercial laboratories for routine effluent testing. This article presents the results of research to develop herring embryo and larval bioassay protocols. Factors evaluated during protocol development included temperature, salinity, dissolved oxygen (DO), light intensity, photoperiod, larval feeding regimes, use of brine and artificial sea salts, gonad sources, collection methods, and egg quality.

  15. Recruitment decline in North Sea herring is accompanied by reduced larval growth rates

    DEFF Research Database (Denmark)

    Payne, Mark; Ross, Stine Dalmann; Worsøe Clausen, Lotte

    2013-01-01

    The stock of North Sea autumn spawning herring (Clupea harengus L.) has shown an unprecedented sequence of ten years of sharply reduced recruitment, in spite of a high spawning biomass. Recent work has identified this below-expected recruitment as being determined during the larval phase: however...... rate of 8%, concurrent with the reduced larval survival and recruitment, was identified: after correcting for the effect of other explanatory variables (e.g. temperature changes), the gross reduction was found to be 12%. This reduction is most probably due to changes in either the amount or quality...... and larvae survival, thereby narrowing the range of potential mechanisms underlying the observed reduction in the recruitment of North Sea autumn spawning herring...

  16. Viability and infectivity of Ichthyophonus sp. in post-mortem Pacific herring, Clupea pallasii.

    Science.gov (United States)

    Kocan, Richard; Hart, Lucas; Lewandowski, Naomi; Hershberger, Paul

    2014-12-01

    Ichthyophonus-infected Pacific herring, Clupea pallasii , were allowed to decompose in ambient seawater then serially sampled for 29 days to evaluate parasite viability and infectivity for Pacific staghorn sculpin, Leptocottus armatus . Ichthyophonus sp. was viable in decomposing herring tissues for at least 29 days post-mortem and could be transmitted via ingestion to sculpin for up to 5 days. The parasite underwent morphologic changes during the first 48 hr following death of the host that were similar to those previously reported, but as host tissue decomposition progressed, several previously un-described forms of the parasite were observed. The significance of long-term survival and continued morphologic transformation in the post-mortem host is unknown, but it could represent a saprozoic phase of the parasite life cycle that has survival value for Ichthyophonus sp.

  17. Protein synthesis, growth and energetics in larval herring (Clupea harengus) at different feeding regimes

    DEFF Research Database (Denmark)

    Houlihan, D F; Pedersen, B H; Steffensen, J F

    1995-01-01

    Rates of growth, protein synthesis and oxygen consumption were measured in herring larvae, Clupea harengus, in order to estimate the contribution that protein synthesis makes to oxygen consumption during rapid growth at 8°C. Protein synthesis rates were determined in larvae 9 to 17 d after hatching....... Larvae were bathed in (3)H phenylalanine for several hours and the free pool and protein-bound phenylalanine specific radioactivities were determined.Fractional rates of protein synthesis increased 5 to 11 fold with feeding after a period of fasting. Efficiencies of retention of synthesized protein were...... approximately 50% during rapid growth. Rapid growth in herring larvae thus appears to be characterized by moderate levels of protein turnover similar to those obtained for larger fish. Increases in growth rate occurred without changes in RNA concentration, i.e., the larvae increased the efficiency of RNA...

  18. An Integrated Assessment Model for Helping the United States Sea Scallop (Placopecten magellanicus) Fishery Plan Ahead for Ocean Acidification and Warming.

    Science.gov (United States)

    Cooley, Sarah R; Rheuban, Jennie E; Hart, Deborah R; Luu, Victoria; Glover, David M; Hare, Jonathan A; Doney, Scott C

    2015-01-01

    Ocean acidification, the progressive change in ocean chemistry caused by uptake of atmospheric CO2, is likely to affect some marine resources negatively, including shellfish. The Atlantic sea scallop (Placopecten magellanicus) supports one of the most economically important single-species commercial fisheries in the United States. Careful management appears to be the most powerful short-term factor affecting scallop populations, but in the coming decades scallops will be increasingly influenced by global environmental changes such as ocean warming and ocean acidification. In this paper, we describe an integrated assessment model (IAM) that numerically simulates oceanographic, population dynamic, and socioeconomic relationships for the U.S. commercial sea scallop fishery. Our primary goal is to enrich resource management deliberations by offering both short- and long-term insight into the system and generating detailed policy-relevant information about the relative effects of ocean acidification, temperature rise, fishing pressure, and socioeconomic factors on the fishery using a simplified model system. Starting with relationships and data used now for sea scallop fishery management, the model adds socioeconomic decision making based on static economic theory and includes ocean biogeochemical change resulting from CO2 emissions. The model skillfully reproduces scallop population dynamics, market dynamics, and seawater carbonate chemistry since 2000. It indicates sea scallop harvests could decline substantially by 2050 under RCP 8.5 CO2 emissions and current harvest rules, assuming that ocean acidification affects P. magellanicus by decreasing recruitment and slowing growth, and that ocean warming increases growth. Future work will explore different economic and management scenarios and test how potential impacts of ocean acidification on other scallop biological parameters may influence the social-ecological system. Future empirical work on the effect of ocean

  19. Molecular identification of erythrocytic necrosis virus (ENV) from the blood of Pacific herring (Clupea pallasii)

    Science.gov (United States)

    Emmenegger, Eveline J.; Glenn, Jolene A.; Winton, James R.; Batts, William N.; Gregg, Jacob L.; Hershberger, Paul K.

    2014-01-01

    Viral erythrocytic necrosis (VEN) is a condition affecting the red blood cells of more than 20 species of marine and anadromous fishes in the North Atlantic and North Pacific Oceans. Among populations of Pacific herring (Clupea pallasii) on the west coast of North America the disease causes anemia and elevated mortality in periodic epizootics. Presently, VEN is diagnosed by observation of typical cytoplasmic inclusion bodies in stained blood smears from infected fish. The causative agent, erythrocytic necrosis virus (ENV), is unculturable and a presumed iridovirus by electron microscopy. In vivo amplification of the virus in pathogen-free laboratory stocks of Pacific herring with subsequent virus concentration, purification, DNA extraction, and high-throughput sequencing were used to obtain genomic ENV sequences. Fragments with the highest sequence identity to the family Iridoviridae were used to design four sets of ENV-specific polymerase chain reaction (PCR) primers. Testing of blood and tissue samples from experimentally and wild infected Pacific herring as well as DNA extracted from other amphibian and piscine iridoviruses verified the assays were specific to ENV with a limit of detection of 0.0003 ng. Preliminary phylogenetic analyses of a 1448 bp fragment of the putative DNA polymerase gene supported inclusion of ENV in a proposed sixth genus of the family Iridoviridae that contains other erythrocytic viruses from ectothermic hosts. This study provides the first molecular evidence of ENV's inclusion within the Iridoviridae family and offers conventional PCR assays as a means of rapidly surveying the ENV-status of wild and propagated Pacific herring stocks.

  20. Temporal patterns of migration and spawning of river herring in coastal Massachusetts

    Science.gov (United States)

    Rosset, Julianne; Roy, Allison; Gahagan, Benjamin I.; Whiteley, Andrew R.; Armstrong, Michael P.; Sheppard, John J.; Jordaan, Adrian

    2017-01-01

    Migrations of springtime Alewife Alosa pseudoharengus and Blueback Herring A. aestivalis, collectively referred to as river herring, are monitored in many rivers along the Atlantic coast to estimate population sizes. While these estimates give an indication of annual differences in the number of returning adults, links to the subsequent timing and duration of spawning and freshwater juvenile productivity remain equivocal. In this study, we captured juvenile river herring at night in 20 coastal Massachusetts lakes using a purse seine and extracted otoliths to derive daily fish ages and back-calculate spawn dates. Estimates of spawning dates were compared with fishway counts of migrating adults to assess differences in migration timing and the timing and duration of spawning. We observed a distinct delay between the beginning of the adult migration run and the start of spawning, ranging from 7 to 28 d across the 20 lakes. Spawning continued 13–48 d after adults stopped migrating into freshwater, further demonstrating a pronounced delay in spawning following migration. Across the study sites the duration of spawning (43–76 d) was longer but not related to the duration of migration (29–66 d). The extended spawning period is consistent with recent studies suggesting that Alewives are indeterminate spawners. The long duration in freshwater provides the opportunity for top-down (i.e., predation on zooplankton) and bottom-up (i.e., food for avian, fish, and other predators) effects, with implications for freshwater food webs and nutrient cycling. General patterns of spawn timing and duration can be incorporated into population models and used to estimate temporal changes in productivity associated with variable timing and density of spawning river herring in lakes.

  1. What can we learn from the stock collapse and recovery of North Sea herring? A review

    DEFF Research Database (Denmark)

    Dickey-Collas, M.; Nash, R.D.M.; Brunel, T.

    The collapse and recovery of North Sea herring in the latter half of the twentieth century hasbeen well documented. With hindsight and the benefit of almost 40 years of extra data andstudies, we can now obtain a clear view of the impact of the collapse and the recovery onthe stock, the fishery, a...... the relevant lessons for the management of other fish stocks and other marine shelf ecosystems...

  2. The use of photon correlation spectroscopy method for determining diffusion coefficient in brine and herring flesh

    Directory of Open Access Journals (Sweden)

    Shumanova M.V.

    2015-03-01

    Full Text Available The process fish salting has been studied by the method of photon correlation spectroscopy; the distribution of salt concentration in the solution and herring flesh with skin has been found, diffusion coefficients and salt concentrations used for creating a mathematical model of the salting technology have been worked out; the possibility of determination by this method the coefficient of dynamic viscosity of solutions and different media (minced meat etc. has been considered

  3. Kinetics of viral shedding provide insights into the epidemiology of viral hemorrhagic septicemia in Pacific herring

    Science.gov (United States)

    Hershberger, Paul K.; Gregg, Jacob L.; Winton, James R.; Grady, Courtney; Collins, Rachael

    2010-01-01

    Losses from infectious diseases are an important component of natural mortality among marine fish species, but factors controlling the ecology of these diseases and their potential responses to anthropogenic changes are poorly understood. We used viral hemorrhagic septicemia virus (VHSV) and a laboratory stock of Pacific herring Clupea pallasii to investigate the kinetics of viral shedding and its effect on disease transmission and host mortality. Outbreaks of acute disease, accompanied by mortality and viral shedding, were initiated after waterborne exposure of herring to concentrations of VHSV as low as 101 plaque-forming units (pfu) ml–1. Shed virus in flow-through tanks was first detected 4 to 5 d post-exposure, peaked after 6 to 10 d, and was no longer detected after 16 d. Shedding rates, calculated from density, flow and waterborne virus titer reached 1.8 to 5.0 × 108 pfu fish–1 d–1. Onset of viral shedding was dose-dependent and preceded initial mortality by 2 d. At 21 d, cumulative mortality in treatment groups ranged from 81 to 100% and was dependent not on challenge dose, but on the kinetics and level of viral shedding by infected fish in the tank. Possible consequences of the viral shedding and disease kinetics are discussed in the context of epizootic initiation and perpetuation among populations of wild Pacific herring.

  4. Persistence of external signs in Pacific herring Clupea pallasii Valenciennes with ichthyophoniasis

    Science.gov (United States)

    Hart, Lucas M.; Conway, Carla M.; Elliott, Diane G.; Hershberger, Paul K.

    2016-01-01

    The progression of external signs of Ichthyophonus infection in Pacific herring Clupea pallasii Valenciennes was highly variable and asynchronous after intraperitoneal injection with pure parasite preparations; however, external signs generally persisted through the end of the study (429 days post-exposure). Observed signs included papules, erosions and ulcers. The prevalence of external signs plateaued 35 days post-exposure and persisted in 73–79% of exposed individuals through the end of the first experiment (147 days post-exposure). Among a second group of infected herring, external signs completely resolved in only 10% of the fish after 429 days. The onset of mortality preceded the appearance of external signs. Histological examination of infected skin and skeletal muscle tissues indicated an apparent affinity of the parasite for host red muscle. Host responses consisted primarily of granulomatous inflammation, fibrosis and necrosis in the skeletal muscle and other tissues. The persistence and asynchrony of external signs and host response indicated that they were neither a precursor to host mortality nor did they provide reliable metrics for hindcasting on the date of exposure. However, the long-term persistence of clinical signs in Pacific herring may be useful in ascertaining the population-level impacts of ichthyophoniasis in regularly observed populations.

  5. Oogenesis, fecundity and condition of Baltic herring (Clupea harengus L.): A stereological study

    DEFF Research Database (Denmark)

    Bucholtz, R. Hagstrøm; Tomkiewicz, J.; Nyengaard, Jens Randel

    2013-01-01

    Herring (Clupea harengus) is a capital breeder that stores energy reserves in muscle tissue. Individual potential fecundity relies on the size and weight of female fish. Poor condition during the maturation process can lead to a heavy down-regulation of fecundity through atresia and, in the extre...... was a good predictor of potential fecundity within maturing stages of females. Combined with estimates of skipped spawning, this ovary weight could be used to estimate egg production thereby improving Central Baltic herring stock-recruitment models.......-regulation of fecundity. Although determined as spring spawners by otolith hatch type, 15% of the randomly sampled females were characterized by oocytes in CA stage in the prespawning period, indicating skipped spawning. The condition of these females was poor, which might have resulted in skipped spawning. Ovary weight......Herring (Clupea harengus) is a capital breeder that stores energy reserves in muscle tissue. Individual potential fecundity relies on the size and weight of female fish. Poor condition during the maturation process can lead to a heavy down-regulation of fecundity through atresia and, in the extreme...

  6. Quantitative determination of creatine kinase release from herring (Clupea harengus) spermatozoa induced by tributyltin.

    Science.gov (United States)

    Grzyb, Katarzyna; Rychłowski, Michał; Biegniewska, Anna; Skorkowski, Edward F

    2003-02-01

    Creatine kinase (CK, ATP creatine phosphotransferase, EC 2.7.3.2) is an enzyme participating in ATP regeneration, which is the primary source of energy in living organisms. We demonstrated that CK from herring spermatozoa has high activity ( approximately 452 micromol/min/g of fresh semen) and has a different electrophoretic mobility from isoenzymes present in skeletal muscle. In our study, we investigated toxic effect of tributyltin (TBT) on herring spermatozoa using a specific sperm viability kit to observe live and dead sperm cells with a confocal microscope. Treatment of herring spermatozoa with TBT caused a time-dependent decrease of viability: 35% nonviable cells with 5 microM TBT and more than 90% nonviable cells with 10 microM TBT after 6 h exposure. We also monitored CK release from damaged spermatozoa into surrounding medium containing different concentrations of TBT. The higher concentration of TBT was used the more CK release from spermatozoa was observed. We suggest that CK could be a good biomarker of sperm cell membranes degradation in the case when lactate dehydrogenase release from permeabilized cells is not possible for rapid determination of the effect of TBT.

  7. Importance of kelp-derived organic carbon to the scallop Chlamys farreri in an integrated multi-trophic aquaculture system

    Science.gov (United States)

    Xu, Qiang; Gao, Fei; Yang, Hongsheng

    2016-03-01

    Bivalves and seaweeds are important cleaners that are widely used in integrated multi-trophic aquaculture (IMTA) systems. A beneficial relationship between seaweed and bivalve in the seaweed-based IMTA system has been confirmed, but the trophic importance of seaweed-derived particulate organic materials to the co-cultured bivalve is still unclear. We evaluated the trophic importance of the kelp Saccharina japonica to the co-cultured scallop Chlamys farreri in a typical IMTA farm in Sungo Bay (Weihai, North China). The dynamics of detritus carbon in the water were monitored during the culturing period. The proportion of kelp-derived organic matter in the diet of the co-cultured scallop was assessed via the stable carbon isotope method. Results showed that the detritus carbon in the water ranged from 75.52 to 265.19 μg/L, which was 25.6% to 73.8% of total particulate organic carbon (TPOC) during the study period. The amount of detritus carbon and its proportion in the TPOC changed throughout the culture cycle of the kelp. Stable carbon isotope analysis showed that the cultured scallop obtained 14.1% to 42.8% of its tissue carbon from the kelp, and that the percentages were closely correlated with the proportion of detritus carbon in the water ( F =0.993, P= 0.003). Evaluation showed that for 17 000 tons (wet weight) of annual scallop production, the kelp contributed about 139.3 tons of carbon (535.8 tons of dry mass). This confirms that cultured kelp plays a similar trophic role in IMTA systems as it does in a natural kelp bed. It is a major contributor to the detritus pool and supplies a vital food source to filter-feeding scallops in the IMTA system, especially during winter and early spring when phytoplankton are scarce.

  8. Scalloped Implant-Abutment Connection Compared to Conventional Flat Implant-Abutment Connection: a Systematic Review and Meta-Analysis.

    Science.gov (United States)

    Starch-Jensen, Thomas; Christensen, Ann-Eva; Lorenzen, Henning

    2017-01-01

    The objective was to test the hypothesis of no difference in implant treatment outcome after installation of implants with a scalloped implant-abutment connection compared to a flat implant-abutment connection. A MEDLINE (PubMed), Embase and Cochrane library search in combination with a hand-search of relevant journals was conducted. No language or year of publication restriction was applied. The search provided 298 titles. Three studies fulfilled the inclusion criteria. The included studies were characterized by low or moderate risk of bias. Survival of suprastructures has never been compared within the same study. High implant survival rate was reported in all the included studies. Significantly more peri-implant marginal bone loss, higher probing depth score, bleeding score and gingival score was observed around implants with a scalloped implant-abutment connection. There were no significant differences between the two treatment modalities regarding professional or patient-reported outcome measures. Meta-analysis disclosed a mean difference of peri-implant marginal bone loss of 1.56 mm (confidence interval: 0.87 to 2.25), indicating significant more bone loss around implants with a scalloped implant-abutment connection. A scalloped implant-abutment connection seems to be associated with higher peri-implant marginal bone loss compared to a flat implant-abutment connection. Therefore, the hypothesis of the present systematic review must be rejected. However, further long-term randomized controlled trials assessing implant treatment outcome with the two treatment modalities are needed before definite conclusions can be provided about the beneficial use of implants with a scalloped implant-abutment connection on preservation of the peri-implant marginal bone level.

  9. Homeopathy outperforms antibiotics treatment in juvenile scallop Argopecten ventricosus: effects on growth, survival, and immune response.

    Science.gov (United States)

    Mazón-Suástegui, José Manuel; García-Bernal, Milagro; Saucedo, Pedro Enrique; Campa-Córdova, Ángel; Abasolo-Pacheco, Fernando

    2017-02-01

    Mortality from vibriosis in mollusk production is attributed to pathogenic bacteria, particularly Vibrio alginolyticus. Use of increasingly potent antibiotics has led to bacterial resistance and increased pathogenicity. Alternatives in sanitation, safety, and environmental sustainability are currently under analysis. To-date, homeopathy has been investigated in aquaculture of freshwater fish, but not in marine mollusks. The effect of the homeopathic complexes in the growth, survival, and immune response of the Catarina scallop Argopecten ventricosus were assessed. A bioassay to assess the potential of homeopathy in improving cultivation of juvenile A. ventricosus was conducted for 21 days, with a final challenge of 120 h with V. alginolyticus. The experimental design included two homeopathic formulas The homeopathic complex Passival, consisting of Passiflora incarnata 30 CH, Valeriana officinalis 30 CH, Ignatia amara 30 CH and Zincum valerianicum 30 CH plus Phosphoricum acid 30 CH (treatment TH1) or Silicea terra 30 CH (TH2), two antibiotics (ampicillin = AMP, oxytetracycline = OXY), and two reference treatments (without homeopathic or antibiotic treatment = CTRL, ethanol 30° GL = ETH). Additionally, a negative control CTRL- (untreated/uninfected) is included in the challenge test. Juvenile scallops (4.14 ± 0.06 mm, 13.33 mg wet weight) were cultivated in 4 L tanks provided with aerated, filtered (1 μm), and UV-sterilized seawater that was changed every third day. They were fed a blend of the microalgae Isochrysis galbana and Chaetoceros calcitrans (150,000 cells mL -1 twice a day). All treatments were directly added to the tank water and then 500 mL challenge units were inoculated with 1 × 10 7  CFU/mL (LD 50 ) of V. alginolyticus. Juveniles grew significantly larger and faster in height and weight with TH2 compared to the ETH and CTRL (P homeopathy is a viable treatment for this mollusk to reduce use of antibiotics in scallops and its

  10. Linking sardine recruitment in coastal areas to ocean currents using surface drifters and HF radar. A case study in the Gulf of Manfredonia, Adriatic Sea

    DEFF Research Database (Denmark)

    Sciascia, Roberta; Berta, Maristella; Carlson, Daniel Frazier

    2017-01-01

    Understanding the role of ocean currents in the recruitment of commercially and ecologically important fish is an important step towards developing sustainable resource management guidelines. To this end, we attempt to elucidate the role of surface ocean transport in supplying recruits of sardine...... (Sardinus pilchardus) to the Gulf of Manfredonia, a known recruitment area in the Adriatic Sea. Sardine early life history stages (ELHS) were collected during two cruises to provide observational estimates of age-size relationship and of their passive pelagic larval duration (PPLD). We combine these PPLDs...... in the Gulf is characterized by repeated pulses from remote SAs. This is the first attempt to describe the processes related to Lagrangian connection to, and retention in, the Gulf of Manfredonia that will be complemented in the future using validated numerical ocean models and biophysical models....

  11. Monitoring programme on toxic metal in bluefish (Pomatomus saltatrix), anchovy (Engraulis encrasicolus) and sardine (Sardina pilchardus) from Istanbul, Turkey: levels and estimated weekly intake.

    Science.gov (United States)

    Özden, Özkan

    2013-05-01

    Toxic metal (Hg, Cd, Pb, Cu and Zn) concentrations of small-medium bluefish, anchovy and sardine in Istanbul, Turkey, were determined using inductively coupled plasma-mass spectrometry (ICP-MS) throughout 1 year. The concentrations of pollutants were found to vary according to season and species. Estimates of weekly intake levels of the metals were calculated and compared to recommended safe limits for fish consumption by humans. The levels of Cd, Pb, Cu and Zn in the fillets of all species resulted in estimates of weekly intake levels that were lower than the recommended safe limits. The concentrations of Hg of small bluefish in September, of medium bluefish in June and September, of anchovy in March, and of sardine in August and September resulted in estimates of weekly intake levels that were higher than the recommended safe limits for human consumption.

  12. Polarographic studies on the nature of cadmium in scallop, oyster, and lobster

    Energy Technology Data Exchange (ETDEWEB)

    Chou, C L; Uthe, J F; Zook, E G

    1978-04-01

    Free and bound forms of cadmium were determined in raw shellfish by use of differential pulse polarography and atomic absorption spectrophotometry. Free cadmium is defined by its polarographic peak potential of -0.62 +- 0.02 V (saturated calomel electrode) in solvent washed ammonium sulfate extracts. Bound cadmium was determined by subtracting the free cadmium from the total cadmium present in the meat. Both scallop (various species) and American lobster (Homarus americanus) muscle tissues contain no free cadmium. Oyster (various species), on the other hand, had a considerable percentage (approximately 50%) of its total cadmium present as free cadmium, a phenomena as yet unexplained. The detection limit for free cadmium is approximately 0.05 ..mu..g/g raw tissue.

  13. Complete mitochondrial genome of the scalloped hammerhead Sphyrna lewini (Carcharhiniformes: Sphyrnidae).

    Science.gov (United States)

    Chen, Xiao; Xiang, Dan; Xu, Yuziwei; Shi, Xiaofang

    2015-08-01

    The complete mitochondrial genome of the endangered scalloped hammerhead Sphyrna lewini was firstly determined in this study. It is 16,726 bp in length with the typical gene composition and orders in vertebrates. The overall base composition is 31.4% A, 26.3% C, 13.2% G and 29.1% T. Two start codon (ATG and GTG) and three stop codon (TAG, AGA and TAA/TA/T) patterns were found in protein-coding genes. Except for the tRNA-Ser2, the remaining 21 tRNAs can be folded into the typical cloverleaf structure. The control region possess the highest A + T content (66.1%) and lowest G content (12.6%) among all mitochondrial partitions.

  14. Variation in size and growth of the great scallop Pecten maximus along a latitudinal gradient.

    Directory of Open Access Journals (Sweden)

    Laurent Chauvaud

    Full Text Available Understanding the relationship between growth and temperature will aid in the evaluation of thermal stress and threats to ectotherms in the context of anticipated climate changes. Most Pecten maximus scallops living at high latitudes in the northern hemisphere have a larger maximum body size than individuals further south, a common pattern among many ectotherms. We investigated differences in daily shell growth among scallop populations along the Northeast Atlantic coast from Spain to Norway. This study design allowed us to address precisely whether the asymptotic size observed along a latitudinal gradient, mainly defined by a temperature gradient, results from differences in annual or daily growth rates, or a difference in the length of the growing season. We found that low annual growth rates in northern populations are not due to low daily growth values, but to the smaller number of days available each year to achieve growth compared to the south. We documented a decrease in the annual number of growth days with age regardless of latitude. However, despite initially lower annual growth performances in terms of growing season length and growth rate, differences in asymptotic size as a function of latitude resulted from persistent annual growth performances in the north and sharp declines in the south. Our measurements of daily growth rates throughout life in a long-lived ectothermic species provide new insight into spatio-temporal variations in growth dynamics and growing season length that cannot be accounted for by classical growth models that only address asymptotic size and annual growth rate.

  15. Feeding and energetics of the great scallop, Pecten maximus, through a DEB model

    Science.gov (United States)

    Lavaud, Romain; Flye-Sainte-Marie, Jonathan; Jean, Fred; Emmery, Antoine; Strand, Øivind; Kooijman, Sebastiaan A. L. M.

    2014-11-01

    We developed a full life-cycle bioenergetic model for the great scallop Pecten maximus relying on the concepts of the Dynamic Energy Budget (DEB) theory. The covariation method was implemented to estimate the parameters of a standard DEB model. Such models are able to predict various metabolic processes from a food availability marker and temperature in the environment. However, suspension-feeders are likely to feed on various trophic sources, from microalgae cells to detritus. They are also able to sort and select food particles very efficiently, depending on their size, energetic value or quality. The present model includes a mechanistic description of the feeding processes, based on Kooijman's Synthesizing Unit principle which allows to deal with several food sources. Moreover we tested the hypothesis of a differential selectivity between two potential substrates (phytoplankton cell and the remaining particulate organic matter). Simulations of shell length, daily shell growth rate, dry weight and gonado-somatic index (GSI) variations were realized and compared to field data from a monitoring conducted in the Bay of Brest (Brittany, France) for six years. The model shows its capacity to efficiently reproduce all life history traits of the wild great scallops. Predicted length data were estimated to the nearest millimeter. The fit of simulated weights to observed data was very satisfactory. GSI predictions were also in accordance with observations but improvements are required to better capture the sharp increase of gametogenesis at the beginning of the year. Finally, results bring evidences that P. maximus is actually preferentially feed on living algae cells rather than on the rest of organic particles.

  16. Marine incursion: the freshwater herring of Lake Tanganyika are the product of a marine invasion into west Africa.

    Directory of Open Access Journals (Sweden)

    Anthony B Wilson

    Full Text Available The spectacular marine-like diversity of the endemic fauna of Lake Tanganyika, the oldest of the African Great Lakes, led early researchers to suggest that the lake must have once been connected to the ocean. Recent geophysical reconstructions clearly indicate that Lake Tanganyika formed by rifting in the African subcontinent and was never directly linked to the sea. Although the Lake has a high proportion of specialized endemics, the absence of close relatives outside Tanganyika has complicated phylogeographic reconstructions of the timing of lake colonization and intralacustrine diversification. The freshwater herring of Lake Tanganyika are members of a large group of pellonuline herring found in western and southern Africa, offering one of the best opportunities to trace the evolutionary history of members of Tanganyika's biota. Molecular phylogenetic reconstructions indicate that herring colonized West Africa 25-50MYA, at the end of a major marine incursion in the region. Pellonuline herring subsequently experienced an evolutionary radiation in West Africa, spreading across the continent and reaching East Africa's Lake Tanganyika during its early formation. While Lake Tanganyika has never been directly connected with the sea, the endemic freshwater herring of the lake are the descendents of an ancient marine incursion, a scenario which may also explain the origin of other Tanganyikan endemics.

  17. The impact of enterocin AS-48 on the shelf-life and safety of sardines (Sardina pilchardus) under different storage conditions.

    Science.gov (United States)

    Ananou, S; Zentar, H; Martínez-Bueno, M; Gálvez, A; Maqueda, M; Valdivia, E

    2014-12-01

    The purpose of this study was to determine the effect of enterocin AS-48, packaged under normal atmosphere (NA), vacuum (VP) or modified atmosphere (MAP) on the shelf life and safety of fresh sardines (Sardina pilchardus) stored at 5 °C. We studied the effect of these hurdles, alone or combined, on the relevant autochthonous bacterial populations. Total volatile basic nitrogen (TVB-N) content was used as indicative of freshness. Levels of biogenic amines cadaverine, putrescine, tyramine, and histamine were also determined. The application of AS-48 did not reduce the mesophilic, psychrotrophic, or Gram negative bacteria viable cell counts under any of the storage conditions tested. AS-48 did cause significant reductions in viable staphylococci counts, especially under VP. In sardines under NA treated with AS-48, the populations of histamine- and tyramine-forming total and lactic acid bacteria (LAB) showed no significant reductions. MAP or VP with AS-48 allowed reductions (significant at some storage times) in histamine- and tyramine-forming LAB. The TVB-N content was also reduced under normal atmosphere and, especially, in sardines stored under MAP. The most interesting results are those concerning the decrease (by several fold) in the levels of the biogenic amines cadaverine, putrescine, tyramine, and histamine determined after treatment with AS-48. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. Oceanic and atmospheric patterns during spawning periods prior to extreme catches of the Brazilian sardine (Sardinella brasiliensis in the southwest Atlantic

    Directory of Open Access Journals (Sweden)

    Helena Cachanhuk Soares

    2011-07-01

    Full Text Available Relative maxima and minima of landings of Brazilian sardine captured in the Southeast Brazil Bight (SBB were compared with oceanic and atmospheric composites relative to the spawning period in December and January, prior to these landings. Atmospheric and oceanic variables such as wind stress, Ekman transport, mixing index, sea surface temperature (SST, precipitation, outgoing long wave radiation and geopotential height were analyzed, revealing distinct climatological patterns in the SBB for these extreme catches that have not been described before. The system could be characterized by cooler SST composite anomaly (SSTA along the SBB as a response to increased cloud cover and reduced incidence of short-wave radiation, predominating one year before the Brazilian sardine catch maxima. This system can take on a different configuration in which positive SSTA condition in the SBB is associated with a less intense South Atlantic Convergence Zone displaced southwards one year before the period of minimum catch. Our results indicate that the spatial structure of the spawning habitat is influenced by specific ocean-atmosphere interactions rather than simply resulting from the choice of a stable environment. This climatic constraint strongly affects the interannual variability of the Brazilian sardine production.

  19. Sex Reversal and Analyses of Possible Involvement of Sex Steroids in Scallop Gonadal Development in Newly Established Organ-Culture Systems.

    Science.gov (United States)

    Otani, Ayano; Nakajima, Tadaaki; Okumura, Tomomi; Fujii, Shiro; Tomooka, Yasuhiro

    2017-04-01

    Many molluscs perform sex reversal, and sex hormones may be involved in the process. In adult scallops, Patinopecten yessoensis, gonadotropin releasing hormone and 17β-estradiol (E 2 ) are involved in male sexual maturation, however, little is known about the effects of E 2 and testosterone (T) on the gonadal differentiation in young scallops. In the present study, scallop gonadal development was analyzed to determine the sex reversal stage in Funka bay, and effects of E 2 and T were examined. In Funka bay, almost all scallops were male at month 12. Scallops equipped with ambiguous gonads were 61.1% at month 16 and disappeared at month 18. Therefore, sex reversal in Funka bay occurs at around month 16. For establishment of organ culture systems for bivalves, Manila clam gonads were cultured in 15% L-15 medium diluted with HBSS containing 10% KSR on agarose gel at 10°C, and the gonads survived for 14 days. Scallop gonads were also able to be cultured in 30% L15 medium diluted with ASW containing 10% KSR on agarose gel for seven days. At mature stage, Foxl2 and Tesk were predominantly expressed in ovary and testis, respectively. When scallop gonads at sex reversal stage were organ-cultured, sex steroid treatment decreased Tesk expression in the majority of scallop gonads at sex reversal stage. However, no obvious change in Foxl2 and Tesk expression was detected in mature gonads in response to either E 2 or T in culture, suggesting sex steroid treatment might affect gonadal development at sex reversal stage.

  20. Comparative biochemical composition in gonad and adductor muscle of triploid and diploid catarina scallop (Argopecten ventricosus Sowerby II, 1842).

    Science.gov (United States)

    Ruiz-Verdugo, C A.; Racotta, I S.; Ibarra, A M.

    2001-05-15

    Biochemical components of gonad and adductor muscle for diploid and triploid catarina scallop, Argopecten ventricosus, were evaluated and compared at four periods in 1 year (January, April, June, and October). Two comparisons were done. The first one compared an untreated control (diploid) vs. a triploidy-treated group for which the percentage of triploids was 57%. The second comparison was done on a group derived from within the triploidy-treated group, separating diploids (internal control) from triploids ('putative triploids'). Regardless of which comparison, in the gonad diploid scallops had larger concentrations of proteins, carbohydrates, lipids, and acylglycerols than triploid scallops. This reflects the maturation processes in diploid scallops vs. the sterility seen in most triploid scallops, and it is particularly supported by the consistently larger concentration of acylglycerols in gonads of diploids than in triploids. The gonad index of the internal control (diploid) group was significantly larger than that seen in the putative triploid group at all sampling periods but October, when none of the gonad biochemical components were different between ploidy groups.Triploid scallops had a significantly larger muscle index than diploids from April to October. This can be caused by a larger gain in muscle tissue in triploids than diploids from January to June. However, there were no consistent differences in any of the biochemical components evaluated in adductor muscle of diploids and triploids. The use of freshly ingested food rather than reserve mobilization from muscle in diploids is suggested by these results. Nutrients derived from ingested food are apparently used for muscle growth in triploids, whereas in diploids those nutrients serve primarily for gonad development. The importance of freshly ingested food for maintenance and growth is suggested because the decline in biochemical components seen in October in both muscle and gonad was paired with a

  1. Fisheries Closed Areas Strengthen Scallop Larval Settlement and Connectivity Among Closed Areas and Across International Open Fishing Grounds: A Model Study

    Science.gov (United States)

    Davies, Kimberley T. A.; Gentleman, W. C.; DiBacco, C.; Johnson, C. L.

    2015-09-01

    This study examined whether a measured increase in average body size of adult sea scallops inside three fishery closed areas on Georges Bank (GB), United States (US), was sufficient to increase larval supply to closed areas and open fishing areas in both US and Canadian areas of the Bank. The effects of adult scallop density-at-size and fecundity-at-size on egg production were compared among open and closed fishery areas, countries, and time periods before and after the closed areas were established. Estimated egg production was then used to define spawning conditions in a coupled biological-physical larval tracking model that simulated larval development, mortality, and dispersal. Results showed that order of magnitude increases in larval settlement after closure were facilitated by increases in size-dependant egg production inside and dispersal from Closed Areas I and II, but not Nantucket Lightship Closed Area. The distributions of both egg production and larval settlement became more uniform across the Bank, causing the relative contribution of Canadian larvae to US scallop aggregations to decrease after establishment of Closed Areas I and II. Decreases in small and medium-sized scallop density in Canada and decreases in large scallops over the US-Southern Flank after closure caused local declines in egg production but were not sufficient to negatively affect larval settlement at the regional scale. Our model suggests that the establishment of fishery closed areas on GB considerably strengthened larval supply and settlement within and among several adult scallop aggregations.

  2. Small scale distribution patterns and vertical migration of North Sea herring larvae (Clupea harengus, Teleostei: Clupeidea in relation to abiotic and biotic factors

    Directory of Open Access Journals (Sweden)

    Holger Haslob

    2009-10-01

    Full Text Available The distribution of herring larvae in relation to environmental conditions and the occurrence of possible prey and predator organisms was studied during a 4-day period on a permanent station in the northern North Sea in September 1999. The vertical distribution of herring larvae was sampled in 20-m intervals by means of a multiple-closing net. To resolve the small-scale patchiness of herring larvae and planktonic prey and predator organisms, a towed in-situ video system was used, the Ichthyoplankton Recorder. A diel vertical migration of herring larvae was observed with different intensities depending on their body length. Small larvae ( 16 mm showed the highest abundances in the upper water layers during the day and were concentrated in deeper water layers during the night. The presented results appear to be relevant for individual-based modelling of the fate of larval herring populations.

  3. Glycerolysis of sardine oil catalyzed by a water dependent lipase in different tert-alcohols as reaction medium

    Directory of Open Access Journals (Sweden)

    Solaesa, Á. G.

    2015-12-01

    Full Text Available The production of monoacylglycerol rich in polyunsaturated fatty acids (PUFA via enzymatic glycerolysis of sardine oil in a homogeneous system was evaluated. Reactions were conducted in two different tert-alcohols. Based on the phase equilibrium data, the amount of solvent added to create a homogeneous system has been calculated and optimized. The immobilized lipase used in this work was Lipozyme RM IM from Rhizomucor miehei, a water dependent lipase. The amount of water added as well as other reaction parameters were studied to evaluate the optimum conditions for monoacylglycerol obtencion. An initial reactant mole ratio glycerol to sardine oil 3:1, 12 wt% of water based on glycerol content and 10 wt% of lipase loading (based on weight of reactants, achieved a MAG yield of around 70%, with nearly 28 wt% PUFA, with low free fatty acid content (lower than 18 wt%.En este trabajo se ha estudiado la producción de monoacilglicéridos, ricos en ácidos grasos poliinsaturados (AGPI, mediante glicerolisis enzimática de aceite de sardina. La reacción se ha llevado a cabo en dos tert-alcoholes para conseguir de esta forma un medio homogéneo de reacción. La cantidad de disolvente añadida al medio de reacción se ha optimizado y calculado en base al equilibrio de fases de los componentes del sistema. La lipasa empleada como biocatalizador ha sido la enzima inmovilizada Lipozyme RM IM de Rhizomucor miehei, una lipasa dependiente de agua. Se ha estudiado el efecto de distintos parámetros cinéticos, así como de la cantidad de agua añadida al medio de reacción, en la producción de monoacilglicéridos. De los resultados obtenidos, se puede concluir que, para una relación molar inicial de reactantes glicerol:aceite de sardina de 3:1, un 12 % en peso de agua en base al glicerol y un 10 % en peso de lipasa, en base al peso de reactantes; se puede llegar a conseguir un rendimiento en monoacilglicéridos alrededor del 70 % en peso, con casi un 28 % en

  4. Effect of baking of sardine (Sardina pilchardus) and frying of anchovy (Engraulis encrasicholus) in olive and sunflower oil on their quality.

    Science.gov (United States)

    Zotos, Anastasios; Kotaras, Akylas; Mikras, Emmanouil

    2013-02-01

    Quality changes due to oven-baking of sardine for 20, 40, 50 and 60 min and due to deep frying of anchovy for 2, 3, 4 and 5 min in olive and sunflower oil were studied. Linear increase in total losses with the time of processing was observed. A linear inverse relationship was observed between moisture/lipid and moisture/protein due to time of baking of sardines and time of frying of anchovies (wet matter). However, no changes were detected in sardine samples due to time of baking (dry matter), while a reduction in proteins and ash followed by an increase in lipids was detected in fried anchovies due to time of frying (dry matter). The fatty acid profiles indicated that a rich in EPA + DHA (33.16%) and in ω-3/ω-6 ratio (9.40) baked sardines can be produced in 20 min at 200 °C. The fatty acid profiles of fried anchovies tremendously changed, indicating entirely different products. Olive oil is probably a better medium to fry fish products, since either the two beneficial fatty acids (EPA and DHA) detected at higher concentrations in anchovies fried in olive oil or the ω-3/ω-6 ratio remained at higher values (0.71-2.56). An increase of cholesterol and squalene content with increasing the time of baking was detected in sardine samples, probably due to decline of moisture content. On the contrary, cholesterol significantly reduced due to frying of anchovy in olive oil. Simultaneously squalene concentration significantly and linearly increased, from 3.87 mg/100 g in the unprocessed anchovies to 73.25 mg/100 g in the samples fried for 5 min, indicating its existence at beneficial levels, besides low cholesterol concentration detected in fried olive oil and squalene concentration gradually and linearly decreased, confirming the absorption from the anchovy samples. Similar was the changes of cholesterol and squalene in anchovies samples fried in sunflower oil.

  5. Low Cost CaTiO3 Perovskite Synthesized from Scallop (Anadara granosa) Shell as Antibacterial Ceramic Material

    Science.gov (United States)

    Fatimah, Is; Nur Ilahi, Rico; Pratami, Rismayanti

    2018-01-01

    Research on perovskite CaTiO3 synthesis from scallop (Anadara granosa) shell and its test as material for antibacterial ceramic application have been conducted. The synthesis was performed by calcium extraction from the scallop shell followed by solid-solid reaction of obtained calcium with TiO2. Physicochemical character of the perovskite wasstudied by measurement of crystallinity using x-ray diffraction (XRD), diffuse-reflectance UV Visible spectrophotometry, scanning electrone microscope-energy dispersive x-ray (SEM-EDX) and Fourier-Transform InfraRed. Considering the future application of the perovskite as antibacterial agent, laboratory test of the peroskite as material in antibacterial ceramic preparation was also conducted. Result of research indicated that perovskite formation was obtained and the material demonstrated photocatalytic activity as identified by band gap energy (Eg) value. The significant activity was also reflected by the antibacterial action of formed ceramic.

  6. Broad-scale climate influences on spring-spawning herring (Clupea harengus, L.) recruitment in the Western Baltic Sea.

    Science.gov (United States)

    Gröger, Joachim P; Hinrichsen, Hans-Harald; Polte, Patrick

    2014-01-01

    Climate forcing in complex ecosystems can have profound implications for ecosystem sustainability and may thus challenge a precautionary ecosystem management. Climatic influences documented to affect various ecological functions on a global scale, may themselves be observed on quantitative or qualitative scales including regime shifts in complex marine ecosystems. This study investigates the potential climatic impact on the reproduction success of spring-spawning herring (Clupea harengus) in the Western Baltic Sea (WBSS herring). To test for climate effects on reproduction success, the regionally determined and scientifically well-documented spawning grounds of WBSS herring represent an ideal model system. Climate effects on herring reproduction were investigated using two global indices of atmospheric variability and sea surface temperature, represented by the North Atlantic Oscillation (NAO) and the Atlantic Multi-decadal Oscillation (AMO), respectively, and the Baltic Sea Index (BSI) which is a regional-scale atmospheric index for the Baltic Sea. Moreover, we combined a traditional approach with modern time series analysis based on a recruitment model connecting parental population components with reproduction success. Generalized transfer functions (ARIMAX models) allowed evaluating the dynamic nature of exogenous climate processes interacting with the endogenous recruitment process. Using different model selection criteria our results reveal that in contrast to NAO and AMO, the BSI shows a significant positive but delayed signal on the annual dynamics of herring recruitment. The westward influence of the Siberian high is considered strongly suppressing the influence of the NAO in this area leading to a higher explanatory power of the BSI reflecting the atmospheric pressure regime on a North-South transect between Oslo, Norway and Szczecin, Poland. We suggest incorporating climate-induced effects into stock and risk assessments and management strategies as part

  7. Broad-scale climate influences on spring-spawning herring (Clupea harengus, L. recruitment in the Western Baltic Sea.

    Directory of Open Access Journals (Sweden)

    Joachim P Gröger

    Full Text Available Climate forcing in complex ecosystems can have profound implications for ecosystem sustainability and may thus challenge a precautionary ecosystem management. Climatic influences documented to affect various ecological functions on a global scale, may themselves be observed on quantitative or qualitative scales including regime shifts in complex marine ecosystems. This study investigates the potential climatic impact on the reproduction success of spring-spawning herring (Clupea harengus in the Western Baltic Sea (WBSS herring. To test for climate effects on reproduction success, the regionally determined and scientifically well-documented spawning grounds of WBSS herring represent an ideal model system. Climate effects on herring reproduction were investigated using two global indices of atmospheric variability and sea surface temperature, represented by the North Atlantic Oscillation (NAO and the Atlantic Multi-decadal Oscillation (AMO, respectively, and the Baltic Sea Index (BSI which is a regional-scale atmospheric index for the Baltic Sea. Moreover, we combined a traditional approach with modern time series analysis based on a recruitment model connecting parental population components with reproduction success. Generalized transfer functions (ARIMAX models allowed evaluating the dynamic nature of exogenous climate processes interacting with the endogenous recruitment process. Using different model selection criteria our results reveal that in contrast to NAO and AMO, the BSI shows a significant positive but delayed signal on the annual dynamics of herring recruitment. The westward influence of the Siberian high is considered strongly suppressing the influence of the NAO in this area leading to a higher explanatory power of the BSI reflecting the atmospheric pressure regime on a North-South transect between Oslo, Norway and Szczecin, Poland. We suggest incorporating climate-induced effects into stock and risk assessments and management

  8. Ecological effects of co-culturing sea cucumber Apostichopus japonicus (Selenka) with scallop Chlamys farreri in earthen ponds

    Science.gov (United States)

    Ren, Yichao; Dong, Shuanglin; Qin, Chuanxin; Wang, Fang; Tian, Xiangli; Gao, Qinfeng

    2012-01-01

    Monthly changes in sedimentation and sediment properties were studied for three different culture treatments: sea cucumber monoculture (Mc), sea cucumber and scallop polyculture (Ps-c) and scallop monoculture (Ms). Results indicated that the survival rate of sea cucumber was significantly higher in Ps-c cultures than in Mc cultures. Sea cucumber yield was 69.6% higher in Ps-c culture than in Mc culture. No significant differences in body weight and scallop shell length were found between Ps-c and Ms cultures. The mean sedimentation rate of total particulate matter (TPM) was 72.2 g/(m2·d) in Ps-c cultures, with a maximum of 119.7 g/(m2·d), which was markedly higher than that of Mc (mean value). Sedimentation rates of organic matter (OM), total organic carbon (TOC), total nitrogen (TN) and total phosphorus (TP) in Ps-c cultures were also significantly higher than those in Mc cultures. TOC and TN contents of sediment increased rapidly in the first 5 months in Ms cultures and remained at a high level. TOC and TN contents in Mc and Ps-c cultures decreased during sea cucumber feeding seasons and increased during sea cucumber dormancy periods (summer and winter). The study demonstrates that co-culture of sea cucumber and scallop in earthen ponds is an alternative way to alleviate nutrient loads and improve water quality in coastal aquaculture systems. Moreover, it provides the additional benefit of an increased sea cucumber yield.

  9. Cornstarch as a dietary supplement in conditioning broodstock and spat at a nursery of the Pacific calico scallop, Argopecten ventricosus

    OpenAIRE

    Mazón-Suástegui, José Manuel; Lodeiros, Cesar; Avilés-Quevedo, Araceli; Rodríguez-Jaramillo, Carmen; Ortiz-Cornejo, Nadia; Abasolo-Pacheco, Fernando

    2017-01-01

    ABSTRACT Microalgae diets supplemented with cornstarch at a rate of 0.15% of live weight to the conditioning of broodstock and spat growth of Pacific calico scallop Argopecten ventricosus were tested. To determine reproductive condition at the beginning and end of the experiment, the gonads of adults were analyzed by stereological methods. For juveniles, the weekly percent increase of spat was estimated from spat biomass volume. The microalgae diet of Tisochrysis lutea and Chaetoceros calcitr...

  10. Spring molybdenum enrichment in scallop shells: a potential tracer of diatom productivity in coastal temperate environments (Brittany, NW France)?

    Science.gov (United States)

    Barats, A.; Amouroux, D.; Pécheyran, C.; Chauvaud, L.; Thébault, J.; Donard, O. F. X.

    2009-08-01

    Skeletal molybdenum/calcium ([Mo]/[Ca])shell ratios were recently examined in bivalves. These ratios were determined by quantitative LA-ICP-MS analyses every third daily striae (i.e. a temporal resolution of 3 days) in 36 flat valves of the Great Scallop shells Pecten maximus (2-year old; 3 shells/year) collected in temperate coastal environments of Western Europe (42 to 49° N). Variations of ([Mo]/[Ca])shell ratio were significant and reproducible for scallops from a same population, from different years (1998-2004) and from different coastal temperate locations. ([Mo]/[Ca])shell exhibits typical profiles characterized by a background content, below the method detection limit (<0.003 μmol/mol) for most of the shell growth period, which is punctuated by a significant transient enrichment (0.031-2.1 μmol/mol) mainly occurring from May to June. The Bay of Brest (France) was especially investigated because of long term observations on scallop communities, environmental variables, and high resolution analyses of dissolved Mo in bottom seawater in 2000. In 2000, dissolved Mo exhibited significant increasing concentration just preceding a maximum of ([Mo]/[Ca])shell ratio. The environmental conditions preceding ([Mo]/[Ca])shell maximum events, both in 2000 and over the 7-year survey indicates a direct influence of the scallop environmental conditions at the sediment water interface subsequent to the intense and periodic spring bloom event. Spring maxima of ([Mo]/[Ca])shell ratio were found to be specifically related to the dynamic of spring diatom blooms and to the extent of the subsequent silicate depletion. ([Mo]/[Ca])shell records reveal thus unexpected biogeochemical routes of Mo, potentially related to coastal spring productivity.

  11. Membrane fractionation of herring marinade for separation and recovery of fats, proteins, amino acids, salt, acetic acid and water

    DEFF Research Database (Denmark)

    Fjerbæk Søtoft, Lene; Lizarazu, Juncal Martin; Razi Parjikolaei, Behnaz

    2015-01-01

    In the production of marinated herring, nearly one ton of acidic saline marinade is produced per 1.5 tons herring fillet. This spent marinade contains highly valuable compounds such as proteins and amino acids. Membranes are suited to recover these substances. In this work, six membrane stages...... containing sugars, amino acids and smaller peptides and a NF permeate containing salt and acetic acid ready for reuse. 42% of the spent marinade is recovered to substitute fresh water and chemicals. The Waste water amount is reduced 62.5%. Proteins are concentrated 30 times, while amino acids and smaller...

  12. An Ichthyophonus hoferi epizootic in herring in the North Sea, the Skagerrak, the Kattegat and the Baltic Sea

    OpenAIRE

    Mellergaard, Stig; Spanggaard, Bettina

    1997-01-01

    An epizootic caused by the internal parasite Ichthyophonus hoferi in herring Clupea harengus was recorded from 1991 to 1993 in the waters around Denmark. A surveillance programme from research vessels and commercial fishing boats was conducted in the North Sea, Skagerrak, Kattegat and Baltic Sea. A total of 15769 hearts of adult herring were examined macroscopically for evidence of infection. The prevalence of the infection in this period decreased from 10.6 to 2.0% in the North Sea, from 8.0...

  13. Hypoxia increases the behavioural activity of schooling herring: a response to physiological stress or respiratory distress?

    DEFF Research Database (Denmark)

    Herbert, Neill A.; Steffensen, John F.

    2006-01-01

    a deviation in physiological homeostasis is associated with any change in behavioural activity, we exposed C. harengus in a school to a progressive stepwise decline in water oxygen pressure  and measured fish swimming speed and valid indicators of primary and secondary stress (i.e. blood cortisol, lactate......Atlantic herring, Clupea harengus, increase their swimming speed during low O2 (hypoxia) and it has been hypothesised that the behavioural response is modulated by the degree of "respiratory distress" (i.e. a rise in anaerobic metabolism and severe physiological stress). To test directly whether...

  14. Modelling the mixing of herring stocks between the Baltic and the North Sea from otolith data

    DEFF Research Database (Denmark)

    Ulrich, Clara; Post, Søren Lorentzen; Worsøe Clausen, Lotte

    2012-01-01

    and Swedish commercial landings and surveys samples for the purpose of stock assessment. But the split estimates from sampling data are highly variable and noisy. Better understanding of the migration and exploitation patterns involved could therefore potentially improve the stock assessment as well...... and are consistent with existing ideas about the migration patterns of WBSS and NSAS within Division IIIa and adjacent waters. This work therefore provides the foundation for the development of a more rational management of the herring stocks in this area...

  15. Predictions of realised fecundity and spawning time in Norwegian spring-spawning herring ( Clupea harengus)

    Science.gov (United States)

    Óskarsson, G. J.; Kjesbu, O. S.; Slotte, A.

    2002-08-01

    Maturing Norwegian spring-spawning (NSS) herring, Clupea harengus, were collected for reproductive analyses along the Norwegian coast prior to the spawning seasons of 1997-2000. Over this time period there was a marked change in weight (W) at length (TL) with 1998 showing extremely low values and 2000 high values in a historical perspective. Potential fecundity, amounting to about 20 000-100 000 developing (vitellogenic) oocytes per fish and positively related to fish size, increased significantly with fish condition. Relative somatic potential fecundity (RF P, number of oocytes per g ovary-free body weight) in NSS herring was found to vary by 35-55% between years. Unexpectedly, females in 2000 showed low RF P-values, possibly due to negative feedback from previous reproductive investments at low condition. A clear threshold value for Fulton's condition factor, K (K=100×W/TL 3), of 0.65-0.70 existed below which there was considerable atresia (resorption of vitellogenic oocytes). Thus, these components of the spawning stock, amounting to 1-46% in the period 1980-1999, obviously contributed relatively little to the total egg production. This was confirmed by low ovary weights and examples of delayed oocyte development in these individuals. An up-to-date atresia model is presented. The established oocyte growth curve, and to a lesser degree the assumed atretic oocytic turnover rate, was critical for the estimation of realised fecundity (number of eggs spawned). Modelled realised fecundity was significantly below observed potential fecundity. Females that had migrated the shortest distance from the over-wintering area, Vestfjorden, northern Norway, were in the poorest condition, had the least developed oocytes and the lowest potential and realised fecundities. In agreement with previously published studies on temporal and spatial changes in gonad weights, those females reaching the main spawning grounds in the south-western part of the coast (Møre) were the most

  16. Apoptosis of hemocytes from lions-paw scallop Nodipecten subnodosus induced with paralyzing shellfish poison from Gymnodinium catenatum.

    Science.gov (United States)

    Estrada, Norma; Ascencio, Felipe; Shoshani, Liora; Contreras, Rubén G

    2014-12-01

    The toxic dinoflagellate Gymnodinium catenatum produces paralyzing shellfish poisons (PSPs) that are consumed and accumulated by bivalves. Previously, we recorded a decrease in hemocytes 24h after injection of PSPs (gonyautoxin 2/3 epimers, GTX2/3) in the adductor muscle in the lions-paw scallop Nodipecten subnodosus. In this work, qualitative and quantitative analyses, in in vivo and in vitro experiments, revealed that the lower count of hemocytes results from cells undergoing typical apoptosis when exposed to GTX 2/3 epimers. This includes visible morphological alterations of the cytoplasmic membrane, damage to the nuclear membrane, condensation of chromatin, DNA fragmentation, and release of DNA fragments into the cytoplasm. Induction of apoptosis was accompanied by phosphatidylserine exposure to the outer cell membrane and activation of cysteine-aspartic proteases, caspase 3 and caspase 8. Addition of an inhibitor of caspase to the medium suppressed activation in hemocytes exposed to the toxins, suggesting that cell death was induced by a caspase-dependent apoptotic pathway. The results are important for future investigation of the scallop's immune system and should provide new insights into apoptotic processes in immune cells of scallops exposed to PSPs. Copyright © 2014 Elsevier GmbH. All rights reserved.

  17. A Marriage Of Larval Modeling And Empirical Data: Linking Adult, Larval And Juvenile Scallops In An Estuary

    Science.gov (United States)

    Bayer, S.; Wahle, R.; Brooks, D. A.; Brady, D. C.

    2016-02-01

    The giant sea scallop, Placopecten magellanicus, is a commercially valuable sedentary broadcast spawner that occupies offshore banks and coastal bays and estuaries in the Northwest Atlantic. Although area closures have helped repopulate depleted scallop populations, little is known about whether populations at densities that yield larvae supply local or distant populations. Surveying scallop populations in the Damariscotta River estuary in Maine during the 2013 and 2014 spawning seasons, and settling out spat bags to collect settling larvae along the gradient of the estuary, we were able to compare adult densities to newly settled juvenile (`spat') abundance. Using the location where we found a high density of adults, we incorporated previously published behavior, pelagic larval duration, wind and current data into a particle dispersal model within the estuary to determine likely sinks for larvae from the 2013 and 2014 spawning seasons. Preliminary model simulations demonstrate where in the estuary swimming is effective in affecting water column position for larvae, and that most larvae are retained much closer to the mouth of the estuary than previously expected. Combining larval dispersal modeling with empirical data on adult densities and spat settlement on the scale of an embayment or estuary may be helpful in determining sources, sinks and areas that are both sources and sinks for shellfish species that are endangered or economically critical. This may aid in determining small area closures or Marine Protected Areas along coastal regions in the Gulf of Maine and beyond.

  18. PENGARUH KATALIS BASA (NaOH PADA TAHAP REAKSI TRANSESTERIFIKASI TERHADAP KUALITAS BIOFUEL DARI MINYAK TEPUNG IKAN SARDIN

    Directory of Open Access Journals (Sweden)

    Diah Probo Ningtyas

    2013-06-01

    Full Text Available Biofuel is an alternative diesel engine fuel is produced from oils/fats of plants and animals (including the fisheries industry waste through the esterification and transesterifiksi reactions. A transesterification is reaction to form esters and glycerol from trigliserin (fat/oil and bioalcohol (methanol or ethanol. Transesterification is an equilibrium reaction so that the presence of a catalyst can accelerate the achievement of a state of equilibrium. Process of the transesterification reaction of sardine flour oil waste with NaOH as base catalyst in producing biofuels was conducted.The research purpose has studied the influence of NaOH concentration in transesterification process and examinate its effect on the quality of biofuels production, conversion, and physic quality. The variables that analysed was the effect of NaOH concentration as catalyst (0.5%, 1.0%, 1.5%, and 2.0% from amount of oil and methanol in the transesterification reaction step. The result showed that the increasing NaOH concentration (0.5 - 1.5%, enhanced the biofuel conversion (%. The highest conversion of biofuels was achieved by using 1.50% NaOH (w/w with 45.34% biofuels conversion. The major component in the biofuels was methyl palmitate (20.31%. ASTM analysis data also supported that the biofuel product was in agreement with automotive diesel fuel specification.

  19. Fluctuations in the spawning stock biomass and recruitment of the brazilian sardine (Sardinella brasiliensis 1977-1997

    Directory of Open Access Journals (Sweden)

    Maria Cristina Cergole

    2002-01-01

    Full Text Available In the southeastern coast of Brazil, catches of the Brazilian sardine (Sardinella brasiliensis have been decreasing from more than 200 thousand tons/year in the early '70s to about 32 thousand tons in 1990. From 1994 to 1997, catches recovered slightly (118 thousand tons, dropping then to about 20 thousand tons in 1999-2000. Extensive investigations on biology and stock assessment of Brazilian sardine have been developed in the '80s, providing stock biomass and recruitment estimates based on commercial fishing data obtained from 1977-1992. The present paper aims to update biological and stock assessment parameters from sardine data obtained during two acoustic surveys carried out in 1995 and commercial catches from the 1993-1997 period. Results from both cruises showed the weight-length relationship Wt=0.0000019. Lt3,26 (90-240 mm total length and four age classes (mostly class II+. In the commercial catches, sardines belonged to ages 0+ and 3+ years old (mostly older than 1-year old with 90-250 mm total length. Annual growth parameters were: Li=275 mm, K= 0.55 (1996, and Li=273 mm, K=0.50 (1997. Total mortality (Z and natural mortality (M coefficients were: Z= 3.8; M= 0.7 -1.2 (1996, and Z= 3.6; M= 0.6 - 1.1 (1997. The variation amplitude of M is due to the application of several different methods. Considering the historical series of 21 years of data (1977-1997, results showed that the stock went through two favorable periods (1980-84, 1989-94 and one unfavorable period (1985-89. 1997 seems to have been the beginning of a new unfavorable period, revealing recruitment cycles likewise other stocks from California and Japan.Na costa sudeste do Brasil, as capturas da sardinha-verdadeira {Sardinella brasliensis decresceram de mais de 200 mil t/ano no início dos anos 70 para cerca de 32 mil t em 1990. De 1994 a 1997, as capturas se recuperaram alcançando 118 mil t, caindo então para cerca de 20 mil t em 1999-2000. Estudos abrangentes sobre a

  20. Certification for Trace Elements and Methyl Mercury Mass Fractions in IAEA-452 Scallop (Pecten maximus) Sample

    International Nuclear Information System (INIS)

    2013-01-01

    to poor environmental management decisions. The IAEA has a long history of organizing interlaboratory studies, which have evolved to include an increasing array of potential contaminants in the marine environment. A marine certified reference material (CRM), IAEA-452, prepared with a scallop (Pecten maximus) sample, was recently produced by the IAEA and certified for trace elements and methylmercury (MeHg). This species of scallop is a common, widely consumed seafood that is also used as a bioindicator for trace metal contamination in marine pollution studies. This publication presents the sample preparation methodology, material homogeneity and stability studies, evaluation of certification campaign results, and assignment of property values and their associated uncertainty. The reference values and associated expanded uncertainty for nine trace elements (As, Cd, Cr, Cu, Fe, Hg, Mn, Pb and Zn) and MeHg in the scallop sample are established. The informative value for one more element (Ni) is also given. The new CRM can be used for the development and validation of analytical methods in the determination of trace elements and MeHg in seafood Pecten maximus as well as for QA/QC purposes

  1. Field comparison of sardine post-flexion larval growth and biochemical composition from three sites in the W Mediterranean (Ebro river coast, bays of Almería and Málaga

    Directory of Open Access Journals (Sweden)

    Alberto Garcia

    2006-10-01

    Full Text Available Late larval stages of sardine (16-23 mm were sampled during the 2003 spawning season in their nursery grounds located off the Ebro river mouth, on the Catalan coast, and in two bays of the Alborán Sea coasts, the Bay of Almería and the Bay of Málaga. The daily growth analysis of each sampled population revealed faster growth in the Ebro sardine larvae than in both of the Alboran Sea larval populations. This fact is supported by their greater content with age of DNA, RNA and protein. However, the significantly higher carbohydrate content of the Bay of Almería sardine larvae and a higher Fulton’s index, indicative of energy storage of individuals, in both of the sardine populations sampled in the Bays of Almería and Málaga show evident differences in the daily growth of the Alborán Sea larvae from those originating in the Ebro region. Late larval growth in the Alborán Sea sardine tends to favour an increase in body mass rather than in body length. This study hypothesises that the productivity pulses off the Alboran Sea coasts induced by the north and northwestern wind regimes may be responsible for the growth pulses observed in the otolith microstructure.

  2. Changes in oxidative stress parameters in relation to age, growth and reproduction in the short-lived catarina scallop Argopecten ventricosus reared in its natural environment.

    Science.gov (United States)

    Guerra, C; Zenteno-Savín, T; Maeda-Martínez, A N; Philipp, E E R; Abele, D

    2012-08-01

    Increase in oxidative damage and decrease in cellular maintenance is often associated with aging, but, in marine ectotherms, both processes are also strongly influenced by somatic growth, maturation and reproduction. In this study, we used a single cohort of the short-lived catarina scallop Argopecten ventricosus, to investigate the effects of somatic growth, reproduction and aging on oxidative damage parameters (protein carbonyls, TBARS and lipofuscin) and cellular maintenance mechanisms (antioxidant activity and apoptosis) in scallops, caged in their natural environment. The concentrations of protein carbonyls and TBARS increased steeply during the early period of fast growth and during reproduction in one-year-old scallops. However, oxidative damage was transient, and apoptotic cell death played a pivotal role in eliminating damage in gill, mantle and muscle tissues of young scallops. Animals were able to reproduce again in the second year, but the reduced intensity of apoptosis impaired subsequent removal of damaged cells. In late survivors low antioxidant capacity and apoptotic activity together with a fast accumulation of the age pigment lipofuscin was observed. Rates of oxygen consumption and oxidative stress markers were strongly dependent on somatic growth and reproductive state but not on temperature. Compared to longer-lived bivalves, A. ventricosus seems more susceptible to oxidative stress with higher tissue-specific protein carbonyl levels and fast accumulation of lipofuscin in animals surviving the second spawning. Superoxide dismutase activity and apoptotic cell death intensity were however higher in this short-lived scallop than in longer-lived bivalves. The life strategy of this short-lived and intensely predated scallop supports rapid somatic growth and fitness as well as early maturation at young age at the cost of fast cellular degradation in second year scallops. Copyright © 2012 Elsevier Inc. All rights reserved.

  3. A small-scale comparison of Iceland scallop size distributions obtained from a camera based autonomous underwater vehicle and dredge survey.

    Directory of Open Access Journals (Sweden)

    Warsha Singh

    Full Text Available An approach is developed to estimate size of Iceland scallop shells from AUV photos. A small-scale camera based AUV survey of Iceland scallops was conducted at a defined site off West Iceland. Prior to height estimation of the identified shells, the distortions introduced by the vehicle orientation and the camera lens were corrected. The average AUV pitch and roll was 1.3 and 2.3 deg that resulted in <2% error in ground distance rendering these effects negligible. A quadratic polynomial model was identified for lens distortion correction. This model successfully predicted a theoretical grid from a frame photographed underwater, representing the inherent lens distortion. The predicted shell heights were scaled for the distance from the bottom at which the photos were taken. This approach was validated by height estimation of scallops of known sizes. An underestimation of approximately 0.5 cm was seen, which could be attributed to pixel error, where each pixel represented 0.24 x 0.27 cm. After correcting for this difference the estimated heights ranged from 3.8-9.3 cm. A comparison of the height-distribution from a small-scale dredge survey carried out in the vicinity showed non-overlapping peaks in size distribution, with scallops of a broader size range visible in the AUV survey. Further investigations are necessary to evaluate any underlying bias and to validate how representative these surveys are of the true population. The low resolution images made identification of smaller scallops difficult. Overall, the observations of very few small scallops in both surveys could be attributed to low recruitment levels in the recent years due to the known scallop parasite outbreak in the region.

  4. A small-scale comparison of Iceland scallop size distributions obtained from a camera based autonomous underwater vehicle and dredge survey.

    Science.gov (United States)

    Singh, Warsha; Örnólfsdóttir, Erla B; Stefansson, Gunnar

    2014-01-01

    An approach is developed to estimate size of Iceland scallop shells from AUV photos. A small-scale camera based AUV survey of Iceland scallops was conducted at a defined site off West Iceland. Prior to height estimation of the identified shells, the distortions introduced by the vehicle orientation and the camera lens were corrected. The average AUV pitch and roll was 1.3 and 2.3 deg that resulted in <2% error in ground distance rendering these effects negligible. A quadratic polynomial model was identified for lens distortion correction. This model successfully predicted a theoretical grid from a frame photographed underwater, representing the inherent lens distortion. The predicted shell heights were scaled for the distance from the bottom at which the photos were taken. This approach was validated by height estimation of scallops of known sizes. An underestimation of approximately 0.5 cm was seen, which could be attributed to pixel error, where each pixel represented 0.24 x 0.27 cm. After correcting for this difference the estimated heights ranged from 3.8-9.3 cm. A comparison of the height-distribution from a small-scale dredge survey carried out in the vicinity showed non-overlapping peaks in size distribution, with scallops of a broader size range visible in the AUV survey. Further investigations are necessary to evaluate any underlying bias and to validate how representative these surveys are of the true population. The low resolution images made identification of smaller scallops difficult. Overall, the observations of very few small scallops in both surveys could be attributed to low recruitment levels in the recent years due to the known scallop parasite outbreak in the region.

  5. Loss of vitamin B(12) in fish (round herring) meats during various cooking treatments.

    Science.gov (United States)

    Nishioka, Michiko; Kanosue, Fuki; Yabuta, Yukinori; Watanabe, Fumio

    2011-01-01

    The loss of vitamin B(12) in round herring meats during various cooking treatments was evaluated. Although amounts of vitamin B(12) were three times greater in the viscera (37.5 ± 10.6 µg/100 g fresh weight) than in the meats, about 73% of total vitamin B(12) found in the whole fish body (except for head and bones) were recovered in the meats (5.1 ± 1.0 µg of vitamin B(12)). The vitamin B(12) contents of the round herring's meats were significantly decreased up to ~62% during cooking by grilling, boiling, frying, steaming, and microwaving. There was, however, no loss of vitamin B(12) during vacuum-packed pouch cooking. Model experiment using hydroxocobalamin suggest that loss of vitamin B(12) is dependent on the degree of temperature and time used in conventional cooking, and is further affected by the concomitant ingredients of food. Retention of vitamin B(12) was not dependent on vacuum or temperature (or both) used in the vacuum-packed pouch cooking.

  6. Morphological effect of a scallop shell on a flapping-type tidal stream generator

    International Nuclear Information System (INIS)

    Le, Tuyen Quang; Ko, Jin Hwan; Byun, Doyoung

    2013-01-01

    Inspired by nature, flapping-type tidal stream generators have been introduced in recent years. The improvement in their power generation ability is known to be a critical factor in the success of these generators. So far, corrugation and camber observed in flying insects and swimming animals are known to enhance the performance of a flapping-type propulsive system. In this study, we explore the effect of corrugation and camber in a system that mimics a scallop shell in terms of its ability to extract flow energy through a two-dimensional Navier–Stokes simulation. The simulations show that the size and the activity of the leading edge vortex are strongly affected by the morphological factors of the mimicked foils, the effects of which are then advantageous in terms of the power efficiency of the flapping-type tidal stream generator. Eventually, an optimal mimicked foil, as suggested based on the morphological effects, would be a good alternative type of foil with a typical section with regard to the hydrodynamic performance and structural properties of tidal stream generators. (paper)

  7. Adaptive scallop height tool path generation for robot-based incremental sheet metal forming

    Science.gov (United States)

    Seim, Patrick; Möllensiep, Dennis; Störkle, Denis Daniel; Thyssen, Lars; Kuhlenkötter, Bernd

    2016-10-01

    Incremental sheet metal forming is an emerging process for the production of individualized products or prototypes in low batch sizes and with short times to market. In these processes, the desired shape is produced by the incremental inward motion of the workpiece-independent forming tool in depth direction and its movement along the contour in lateral direction. Based on this shape production, the tool path generation is a key factor on e.g. the resulting geometric accuracy, the resulting surface quality, and the working time. This paper presents an innovative tool path generation based on a commercial milling CAM package considering the surface quality and working time. This approach offers the ability to define a specific scallop height as an indicator of the surface quality for specific faces of a component. Moreover, it decreases the required working time for the production of the entire component compared to the use of a commercial software package without this adaptive approach. Different forming experiments have been performed to verify the newly developed tool path generation. Mainly, this approach serves to solve the existing conflict of combining the working time and the surface quality within the process of incremental sheet metal forming.

  8. Expression pattern of phb2 and its potential function in spermatogenesis of scallop ( Chlamys farreri)

    Science.gov (United States)

    Han, Tiantian; Ma, Xiaoshi; Liang, Shaoshuai; Gao, Beibei; Zhang, Zhifeng

    2015-12-01

    Prohibitin (PHB) participates in several biological processes including apoptosis, transcription regulation and suppression of cell proliferation in mammals. In this study, we cloned the full-length cDNA of prohibitin 2 ( Cf-phb2) from the testis of scallop ( Chlamys farreri). The deduced amino acid sequence presented a characteristic of PHB family with the PHB domain, and clustered with PHB2 of other species. Temporal and spatial expression of Cf-phb2 in testis during the reproductive cycle was detected by quantitative real-time PCR (qRT-PCR) and in situ hybridization. The expression of Cf-phb2 in the testis increased when testis developed from the resting stage to mature stage. The mRNA abundance of Cf-phb2 was the highest at mature stage, which was about 15-fold higher than that at proliferative stage. The expression of Cf-phb2 could be detected by in situ hybridization in all types of germ cells in testis, including spermatogonia, spermatocytes, spermatids and spermatozoa. The intensity of the signal increased with the spermatogenesis and was the highest in spermatids, which suggested that CF-PHB2 might affect the spermatogenesis of C. farreri.

  9. Larval and juvenile Pacific herring Clupea pallasii are not susceptible to infectious hematopoietic necrosis under laboratory conditions

    Science.gov (United States)

    Hart, L.M.; Traxler, G.S.; Garver, K.A.; Richard, J.; Gregg, J.L.; Grady, C.A.; Kurath, G.; Hershberger, P.K.

    2011-01-01

    Infectious hematopoietic necrosis (IHN) leads to periodic epidemics among certain wild and farmed fish species of the Northeast (NE) Pacific. The source of the IHN virus (IHNV) that initiates these outbreaks remains unknown; however, a leading hypothesis involves viral persistence in marine host species such as Pacific herring Clupea pallasii. Under laboratory conditions we exposed specific pathogen-free (SPF) larval and juvenile Pacific herring to 103 to 104 plaque-forming units (pfu) of IHNV ml–1 by waterborne immersion. Cumulative mortalities among exposed groups were not significantly different from those of negative control groups. After waterborne exposure, IHNV was transiently recovered from the tissues of larvae but absent in tissues of juveniles. Additionally, no evidence of viral shedding was detected in the tank water containing exposed juveniles. After intraperitoneal (IP) injection of IHNV in juvenile herring with 103 pfu, IHNV was recovered from the tissues of sub-sampled individuals for only the first 5 d post-exposure. The lack of susceptibility to overt disease and transient levels of IHNV in the tissues of exposed fish indicate that Pacific herring do not likely serve a major epizootiological role in perpetuation of IHNV among free-ranging sockeye salmon Oncorhynchus nerka and farmed Atlantic salmon Salmo salar in the NE Pacific.

  10. Investigation of spatial trends and neurochemical impacts of mercury in herring gulls across the Laurentian Great Lakes

    Energy Technology Data Exchange (ETDEWEB)

    Rutkiewicz, Jennifer [Department of Environmental Health Sciences, University of Michigan School of Public Health, 109 S. Observatory St, Ann Arbor, MI 48109 (United States); Scheuhammer, Anton; Crump, Doug; Jagla, Magdalena [Environment Canada, National Wildlife Research Centre, Carleton University, Ottawa, ON K1A 0H3 (Canada); Basu, Niladri, E-mail: niladri@umich.ed [Department of Environmental Health Sciences, University of Michigan School of Public Health, 109 S. Observatory St, Ann Arbor, MI 48109 (United States)

    2010-08-15

    Herring gulls (Larus argentatus) bioaccumulate mercury (Hg) but it is unknown whether they are exposed at levels of neurological concern. Here we studied brain tissues from gulls at five Great Lakes colonies and one non-Great Lakes colony during spring of 2001 and 2003. Total brain Hg concentrations ranged from 0.14 to 2.0 {mu}g/g (dry weight) with a mean of 0.54 {mu}g/g. Gulls from Scotch Bonnet Island, on the easternmost edge of the Great Lakes, had significantly higher brain Hg than other colonies. No association was found between brain Hg concentration and [3H]-ligand binding to neurochemical receptors (N-methyl-D-aspartate, muscarinic cholinergic, nicotinic cholinergic) or nicotinic receptor {alpha}-7 relative mRNA expression as previously documented in other wildlife. In conclusion, spatial trends in Hg contamination exist in herring gulls across the Great Lakes basin, and herring gulls accumulate brain Hg but not at levels associated with sub-clinical neurochemical alterations. - Spatial trends in brain mercury exist in herring gulls across the Laurentian Great Lakes though levels are not associated with neurochemical biomarkers.

  11. Coupling otolith microstructure analysis and hydrographic backtracking suggests a mechanism for the 2000s North Sea herring recruitment failure

    DEFF Research Database (Denmark)

    Ross, Stine Dalmann; Payne, Mark; Worsøe Clausen, Lotte

    2012-01-01

    The North Sea autumn spawning herring (Clupea harengus) has, since the 2002 year class, shown an unprecedented sequence of ten years of sharply reduced recruitment, in spite of a high spawning biomass and low fishing mortality. Recent work has identified this reduction in recruitment level (or st...

  12. Characterization of herring populations west of the British Isles: an investigation of mixing based on otolith microchemistry

    NARCIS (Netherlands)

    Geffen, A.J.; Nash, R.D.M.; Dickey-Collas, M.

    2011-01-01

    Herring along the west coast of the British Isles are managed and assessed as a series of discrete stocks. The relationship between the spawning components, mixed (feeding) aggregations, and juveniles in nursery areas for these stocks was modelled by discriminant analysis and integrated stock

  13. Regional and seasonal differences in growth of larval North Sea herring (clupea harengus L.) estimated by otolith microstructure analysis

    DEFF Research Database (Denmark)

    Munk, Peter; Heath, Mike; Skaarup, Bo

    1991-01-01

    The ecology processes of the larval life of autumn-spawned North Sea herring have been studied in a multidisciplinary and internationally coordinated research programme (ACE). The programme focused on larval advection and the importance of the autumn/winter circulation in determining larval distr...

  14. What is left? Macrophyte meadows and Atlantic herring (Clupea harengus) spawning sites in the Greifswalder Bodden, Baltic Sea

    Science.gov (United States)

    Kanstinger, Philipp; Beher, Jutta; Grenzdörffer, Görres; Hammer, Cornelius; Huebert, Klaus B.; Stepputis, Daniel; Peck, Myron A.

    2018-02-01

    Coastal zones are productive areas of marine ecosystems which are also hotspots of anthropogenic activities causing habitat degradation. In the southwest Baltic Sea, eutrophication is thought to have caused the massive reduction in submerged macrophytes observed in recent decades. Here, we surveyed the submarine vegetation and examined locations of spawning of herring (Clupea harengus) in the Greifswalder Bodden, one of the most important reproductive habitats of the Western Baltic Spring Spawner herring stock (WBSS). This stock deposits eggs onto submerged vegetation and changes in macrophyte coverage are expected to influence the availability of reproductive habitat. Aerial, underwater video tows and SCUBA surveys conducted in spring 2009 revealed that only ∼7% of the lagoon was vegetated. Herring eggs were observed on 12 of 32 SCUBA transects, at depths between 0.2 and 5 m and were attached to a variety of spermatophyte and algae species but not to stones or mussels. A classification tree model indicated that spawning sites were strongly associated with the vegetation cover within a 100- and 500-m radius, implying that herring schools preferentially spawn on dense and large underwater meadows. Only ∼5% of the lagoon now falls into this vegetation category. Despite 20 years of efforts to reduce eutrophication, no increase in macroalgae and spermatophyte vegetation towards the historical level of 90% coverage in the area is apparent.

  15. Effects of temperature and population density on von Bertalanffy growth parameters in Atlantic herring: a macro-ecological analysis

    NARCIS (Netherlands)

    Brunel, T.P.A.; Dickey-Collas, M.

    2010-01-01

    The effect of temperature and population density on the growth of Atlantic herring Clupea harengus was studied using a comparative approach applied to 15 North Atlantic populations. The von Bertalanffy (VB) equation was applied to describe mean growth of individuals in each population, both averaged

  16. Microbiological quality of maatjes herring stored in air and under modified atmosphere at 4 and 10 C

    NARCIS (Netherlands)

    Lyhs, U.; Lahtinen, J.; Schelvis-Smit, A.A.M.

    2007-01-01

    Microbiological and sensory changes of maatjes herring stored in air (experiment I) and under modified atmosphere (MAP) (experiments II and III) were evaluated during storage at 4 and 10 °C. Microbial (total and psychrotrophic viable bacteria, lactic acid bacteria and Enterobacteriaceae) counts and

  17. Subchronic toxicity of Baltic herring oil and its fractions in the rat II: Clinical observations and toxicological parameters

    NARCIS (Netherlands)

    Stern, N.H.; Oberg, M.; Casabona, H.; Trossvik, C.; Manzoor, E.; Johansson, N.; Lind, M.; Orberg, J.; Feinstein, R.; Johansson, A.; Chu, I.; Poon, R.; Yagminas, A.; Brouwer, A.; Jones, B.; Hakansson, H.

    2002-01-01

    This study aimed to increase the knowledge about the toxicity of fish-derived organohalogen pollutants in mammals. The strategy chosen was to separate organohalogen pollutants derived from Baltic herring (Clupea harengus) fillet, in order to obtain fractions with differing proportions of identified

  18. An Ichthyophonus hoferi epizootic in herring in the North Sea, the Skagerrak, the Kattegat and the Baltic Sea

    DEFF Research Database (Denmark)

    Mellergaard, Stig; Spanggaard, Bettina

    1997-01-01

    An epizootic caused by the internal parasite Ichthyophonus hoferi in herring Clupea harengus was recorded from 1991 to 1993 in the waters around Denmark. A surveillance programme from research vessels and commercial fishing boats was conducted in the North Sea, Skagerrak, Kattegat and Baltic Sea...

  19. Investigation of spatial trends and neurochemical impacts of mercury in herring gulls across the Laurentian Great Lakes

    International Nuclear Information System (INIS)

    Rutkiewicz, Jennifer; Scheuhammer, Anton; Crump, Doug; Jagla, Magdalena; Basu, Niladri

    2010-01-01

    Herring gulls (Larus argentatus) bioaccumulate mercury (Hg) but it is unknown whether they are exposed at levels of neurological concern. Here we studied brain tissues from gulls at five Great Lakes colonies and one non-Great Lakes colony during spring of 2001 and 2003. Total brain Hg concentrations ranged from 0.14 to 2.0 μg/g (dry weight) with a mean of 0.54 μg/g. Gulls from Scotch Bonnet Island, on the easternmost edge of the Great Lakes, had significantly higher brain Hg than other colonies. No association was found between brain Hg concentration and [3H]-ligand binding to neurochemical receptors (N-methyl-D-aspartate, muscarinic cholinergic, nicotinic cholinergic) or nicotinic receptor α-7 relative mRNA expression as previously documented in other wildlife. In conclusion, spatial trends in Hg contamination exist in herring gulls across the Great Lakes basin, and herring gulls accumulate brain Hg but not at levels associated with sub-clinical neurochemical alterations. - Spatial trends in brain mercury exist in herring gulls across the Laurentian Great Lakes though levels are not associated with neurochemical biomarkers.

  20. GPS tracking data of Lesser Black-backed Gulls and Herring Gulls breeding at the southern North Sea coast

    NARCIS (Netherlands)

    Stienen, E.W.M.; Desmet, P.; Aelterman, B.; Courtens, W.; Feys, S.; Vanermen, N.; Verstraete, H.; Van de Walle, M.; Deneudt, K.; Hernandez, F.; Houthoofdt, R.; Vanhoorne, B.; Bouten, W.; Buijs, R.-J.; Kavelaars, M.M.; Müller, W.; Herman, D.; Matheve, H.; Sotillo, A.; Lens, L.

    2016-01-01

    In this data paper, Bird tracking - GPS tracking of Lesser Black-backed Gulls and Herring Gulls breeding at the southern North Sea coast is described, a species occurrence dataset published by the Research Institute for Nature and Forest (INBO). The dataset (version 5.5) contains close to 2.5

  1. Herring and chicken/pork meals lead to differences in plasma levels of TCA intermediates and arginine metabolites in overweight and obese men and women

    DEFF Research Database (Denmark)

    Vincent, Andrew; Savolainen, Otto I; Sen, Partho

    2017-01-01

    citrate, fumarate, isocitrate, glycolate, oxalate, agmatine and methyhistidine and increased asparagine, ornithine, glutamine and the hexosamine glucosamine. Modelling found that the tricarboxylic acid cycle, glyoxylate, and argininemetabolism were affected by the intervention. The effect on arginine...... metabolism was supported by an increase in blood nitric oxide in males on the herring diet. Conclusion: The results suggest that eating herring instead of chicken and lean pork leads to important metabolic effects, particularly on energy and amino acid metabolism. Our findings support the hypothesis...... that there are metabolic effects of herring intake unrelated to the long chain n-3 polyunsaturated fatty acid content....

  2. Fish protein hydrolysate production from sardine solid waste by crude pepsin enzymatic hydrolysis in a bioreactor coupled to an ultrafiltration unit

    International Nuclear Information System (INIS)

    Benhabiles, M.S.; Abdi, N.; Drouiche, N.; Lounici, H.; Pauss, A.; Goosen, M.F.A.; Mameri, N.

    2012-01-01

    The aims of the study were to optimize the production a fish protein hydrolysate (FPH) by enzymatic hydrolysis of sardine solid waste using crude pepsin, and to scale up the process in a bioreactor coupled to an ultrafiltration unit for product recovery. Results showed that the crude pepsin prepared by autolysis of the mucous membranes of a sheep stomach at optimal conditions (i. e. pH = 1.5–2 and incubation time of 6 h) could be satisfactory used for the enzymatic hydrolysis of fish solid waste. The optimal conditions for enzymatic reaction were: temperature 48 °C, and pH 1.5. The scale up of the enzymatic hydrolysis and the coupling of the reactor an ultrafiltration unit to concentrate the hydrolysate gave good results with a rejection coefficient for the protein hydrolysate product in the range of 90%. The volumetric concentration factor was 2.5, with a permeate flux of 200 L m −2 bar −1 . However, the results also suggest that the ultrafiltration product concentration process may be operating beyond the critical flux at which point irreversible membrane fouling occurs. - Highlights: ► Evaluating to produce a (FPH) by enzymatic hydrolysis of sardine solid wastes was achieved. ► Investigation of key parameters for optimal conditions for enzymatic hydrolysis have been studied. ► Valorization of sardine waste was realized by enzymatic hydrolysis process. ► Performances of this enzyme gave comparable results to those obtained with commercial pepsin. ► The nutritional quality of the FPH produced appears to be satisfactory.

  3. What can otolith shape analysis tell us about population structure of the European sardine, Sardina pilchardus, from Atlantic and Mediterranean waters?

    Science.gov (United States)

    Jemaa, Sharif; Bacha, Mahmoud; Khalaf, Gaby; Dessailly, David; Rabhi, Khalef; Amara, Rachid

    2015-02-01

    The European sardine, Sardina pilchardus, exhibits a complex population structure, which has produced conflicting results in previous genetic studies. Despite its importance in the fisheries industry, stock delineation for management and conservation purposes is still a matter of debate throughout the distribution range of the species. This study examines whether otolith shapes are more efficient than genetic markers to detect population structure in pelagic species with large population sizes. Sardines were analyzed from 15 sampling localities in the Northeast Atlantic and Mediterranean Sea covering almost the whole distribution range of the species. A combination of otolith shape indices and elliptic Fourier descriptors was investigated by multivariate statistical procedures. Within the studied area, three distinct groups were identified with an overall correct classification of 77%. Group A: northern Mediterranean Sea and Gulf of Gabès; group B: Atlantic Morocco-south Alboran-Algero-provençal coasts; and group C: European Atlantic coast. The Almeria-Oran front and the Gibraltar strait are not an efficient barrier for sardine population separation as there seems to be exchanges between populations of the south-western Mediterranean Sea and those of the Moroccan Atlantic Ocean coast or Gulf of Cadiz. The results are discussed in relation to environmental conditions, oceanographic features, and physical barriers to dispersal in the study area, and compared with those obtained by previous genetic, morphometric, and meristic data. For pelagic species with high gene flow, present results highlighted the need to take into account the identification of phenotypic stocks to ensure sustainable fishery benefits and efficient conservation as they may have unique demographic properties and responses to exploitation.

  4. Fish protein hydrolysate production from sardine solid waste by crude pepsin enzymatic hydrolysis in a bioreactor coupled to an ultrafiltration unit

    Energy Technology Data Exchange (ETDEWEB)

    Benhabiles, M.S.; Abdi, N. [National Polytechnic school of Algiers, B.P. 182-16200, El Harrach, Algiers (Algeria); Drouiche, N., E-mail: nadjibdrouiche@yahoo.fr [National Polytechnic school of Algiers, B.P. 182-16200, El Harrach, Algiers (Algeria); Silicon Technology Development Unit (UDTS) 2, Bd Frantz Fanon BP140, Alger-7 Merveilles, 16000 (Algeria); Lounici, H. [National Polytechnic school of Algiers, B.P. 182-16200, El Harrach, Algiers (Algeria); Pauss, A. [University of Technology of Compiegne, Departement Genie chimique,B.P. 20.509, 60205 Compiegne cedex (France); Goosen, M.F.A. [Alfaisal University, Riyadh (Saudi Arabia); Mameri, N. [University of Technology of Compiegne, Departement Genie chimique,B.P. 20.509, 60205 Compiegne cedex (France)

    2012-05-01

    The aims of the study were to optimize the production a fish protein hydrolysate (FPH) by enzymatic hydrolysis of sardine solid waste using crude pepsin, and to scale up the process in a bioreactor coupled to an ultrafiltration unit for product recovery. Results showed that the crude pepsin prepared by autolysis of the mucous membranes of a sheep stomach at optimal conditions (i. e. pH = 1.5-2 and incubation time of 6 h) could be satisfactory used for the enzymatic hydrolysis of fish solid waste. The optimal conditions for enzymatic reaction were: temperature 48 Degree-Sign C, and pH 1.5. The scale up of the enzymatic hydrolysis and the coupling of the reactor an ultrafiltration unit to concentrate the hydrolysate gave good results with a rejection coefficient for the protein hydrolysate product in the range of 90%. The volumetric concentration factor was 2.5, with a permeate flux of 200 L m{sup -2} bar{sup -1}. However, the results also suggest that the ultrafiltration product concentration process may be operating beyond the critical flux at which point irreversible membrane fouling occurs. - Highlights: Black-Right-Pointing-Pointer Evaluating to produce a (FPH) by enzymatic hydrolysis of sardine solid wastes was achieved. Black-Right-Pointing-Pointer Investigation of key parameters for optimal conditions for enzymatic hydrolysis have been studied. Black-Right-Pointing-Pointer Valorization of sardine waste was realized by enzymatic hydrolysis process. Black-Right-Pointing-Pointer Performances of this enzyme gave comparable results to those obtained with commercial pepsin. Black-Right-Pointing-Pointer The nutritional quality of the FPH produced appears to be satisfactory.

  5. 210Po and 210Pb trophic transfer within the phytoplankton–zooplankton–anchovy/sardine food web: a case study from the Gulf of Lion (NW Mediterranean Sea)

    International Nuclear Information System (INIS)

    Strady, Emilie; Harmelin-Vivien, Mireille; Chiffoleau, Jean François; Veron, Alain; Tronczynski, Jacek; Radakovitch, Olivier

    2015-01-01

    The transfer of 210 Po and 210 Pb in the food web of small pelagic fishes (from phytoplankton and zooplankton to anchovy Engraulis encrasicolus and sardine Sardina pilchardus) is investigated in the Gulf of Lion (GoL). We present original data of 210 Po and 210 Pb activity concentrations, C and N stable isotope ratios, measured (i) from different size classes of phytoplankton and zooplankton during spring and winter in different environments of the GoL, and (ii) in two fish species. Significant spatial patterns based on 210 Po, 210 Pb activity concentrations and 210 Po/ 210 Pb ratios in the different plankton size classes are evidenced by hierarchical clustering, both in spring and winter. This variability, also observed for C and N stable isotopes ratios, is connected to local specific pelagic habitats and hydrodynamics. The sampling strategy suggests that 210 Po bioaccumulation in the GoL remains at a constant level from the first (dominated by phytoplankton) to the second trophic level (zooplankton), while 210 Pb bioaccumulation shows an increase in winter. Based on stable N isotope ratios and 210 Po activity concentrations measured in anchovies and sardines, we evidence 210 Po bio-magnification along the trophic food web of these two planktivorous pelagic fishes. - Highlights: • 210 Po and 210 Pb activity concentrations in plankton vary up to a factor of two in the Gulf of Lion (East vs West). • 210 Po and 210 Pb variability is connected to local specific pelagic habitats. • Bio-magnification of 210 Po is evidenced in anchovy/sardine foodwebs

  6. Stomach Chitinase from Japanese Sardine Sardinops melanostictus: Purification, Characterization, and Molecular Cloning of Chitinase Isozymes with a Long Linker

    Directory of Open Access Journals (Sweden)

    Satoshi Kawashima

    2016-01-01

    Full Text Available Fish express two different chitinases, acidic fish chitinase-1 (AFCase-1 and acidic fish chitinase-2 (AFCase-2, in the stomach. AFCase-1 and AFCase-2 have different degradation patterns, as fish efficiently degrade chitin ingested as food. For a comparison with the enzymatic properties and the primary structures of chitinase isozymes obtained previously from the stomach of demersal fish, in this study, we purified chitinase isozymes from the stomach of Japanese sardine Sardinops melanostictus, a surface fish that feeds on plankton, characterized the properties of these isozymes, and cloned the cDNAs encoding chitinases. We also predicted 3D structure models using the primary structures of S. melanostictus stomach chitinases. Two chitinase isozymes, SmeChiA (45 kDa and SmeChiB (56 kDa, were purified from the stomach of S. melanostictus. Moreover, two cDNAs, SmeChi-1 encoding SmeChiA, and SmeChi-2 encoding SmeChiB were cloned. The linker regions of the deduced amino acid sequences of SmeChi-1 and SmeChi-2 (SmeChi-1 and SmeChi-2 are the longest among the fish stomach chitinases. In the cleavage pattern groups toward short substrates and the phylogenetic tree analysis, SmeChi-1 and SmeChi-2 were classified into AFCase-1 and AFCase-2, respectively. SmeChi-1 and SmeChi-2 had catalytic domains that consisted of a TIM-barrel (β/α8–fold structure and a deep substrate-binding cleft. This is the first study showing the 3D structure models of fish stomach chitinases.

  7. Feeding Ecology of Northeast Atlantic Mackerel, Norwegian Spring-Spawning Herring and Blue Whiting in the Norwegian Sea.

    Directory of Open Access Journals (Sweden)

    Eneko Bachiller

    Full Text Available The Norwegian spring-spawning (NSS herring (Clupea harengus, blue whiting (Micromesistius poutassou and Northeast Atlantic (NEA mackerel (Scomber scombrus are extremely abundant pelagic planktivores that feed in the Norwegian Sea (NS during spring and summer. This study investigated the feeding ecology and diet composition of these commercially important fish stocks on the basis of biological data, including an extensive set of stomach samples in combination with hydrographical data, zooplankton samples and acoustic abundance data from 12 stock monitoring surveys carried out in 2005-2010. Mackerel were absent during the spring, but had generally high feeding overlap with herring in the summer, with a diet mainly based on calanoid copepods, especially Calanus finmarchicus, as well as a similar diet width. Stomach fullness in herring diminished from spring to summer and feeding incidence was lower than that of mackerel in summer. However, stomach fullness did not differ between the two species, indicating that herring maintain an equally efficient pattern of feeding as mackerel in summer, but on a diet that is less dominated by copepods and is more reliant on larger prey. Blue whiting tended to have a low dietary overlap with mackerel and herring, with larger prey such as euphausiids and amphipods dominating, and stomach fullness and feeding incidence increasing with length. For all the species, feeding incidence increased with decreasing temperature, and for mackerel so did stomach fullness, indicating that feeding activity is highest in areas associated with colder water masses. Significant annual effects on diet composition and feeding-related variables suggested that the three species are able to adapt to different food and environmental conditions. These annual effects are likely to have an important impact on the predation pressure on different plankton groups and the carrying capacity of individual systems, and emphasise the importance of

  8. Feeding Ecology of Northeast Atlantic Mackerel, Norwegian Spring-Spawning Herring and Blue Whiting in the Norwegian Sea.

    Science.gov (United States)

    Bachiller, Eneko; Skaret, Georg; Nøttestad, Leif; Slotte, Aril

    2016-01-01

    The Norwegian spring-spawning (NSS) herring (Clupea harengus), blue whiting (Micromesistius poutassou) and Northeast Atlantic (NEA) mackerel (Scomber scombrus) are extremely abundant pelagic planktivores that feed in the Norwegian Sea (NS) during spring and summer. This study investigated the feeding ecology and diet composition of these commercially important fish stocks on the basis of biological data, including an extensive set of stomach samples in combination with hydrographical data, zooplankton samples and acoustic abundance data from 12 stock monitoring surveys carried out in 2005-2010. Mackerel were absent during the spring, but had generally high feeding overlap with herring in the summer, with a diet mainly based on calanoid copepods, especially Calanus finmarchicus, as well as a similar diet width. Stomach fullness in herring diminished from spring to summer and feeding incidence was lower than that of mackerel in summer. However, stomach fullness did not differ between the two species, indicating that herring maintain an equally efficient pattern of feeding as mackerel in summer, but on a diet that is less dominated by copepods and is more reliant on larger prey. Blue whiting tended to have a low dietary overlap with mackerel and herring, with larger prey such as euphausiids and amphipods dominating, and stomach fullness and feeding incidence increasing with length. For all the species, feeding incidence increased with decreasing temperature, and for mackerel so did stomach fullness, indicating that feeding activity is highest in areas associated with colder water masses. Significant annual effects on diet composition and feeding-related variables suggested that the three species are able to adapt to different food and environmental conditions. These annual effects are likely to have an important impact on the predation pressure on different plankton groups and the carrying capacity of individual systems, and emphasise the importance of regular

  9. Characterization of AhR agonists reveals antagonistic activity in European herring gull (Larus argentatus) eggs.

    Science.gov (United States)

    Muusse, Martine; Christensen, Guttorm; Gomes, Tânia; Kočan, Anton; Langford, Katherine; Tollefsen, Knut Erik; Vaňková, Lenka; Thomas, Kevin V

    2015-05-01

    European herring gull (Larus argentatus) eggs from two Norwegian islands, Musvær in the south east and Reiaren in Northern Norway, were screened for dioxins, furans, and dioxin-like and selected non-dioxin-like polychlorinated biphenyls (PCBs), and subjected to non-target analysis to try to identify the aryl hydrocarbon receptor (AhR) agonists, responsible for elevated levels measured using the dioxin responsive chemically activated luciferase expression (DR-CALUX) assay. Eggs from Musvær contained chemically calculated toxic equivalent (WHO TEQ) levels of between 109 and 483 pg TEQ/g lw, and between 82 and 337 pg TEQ/g lw was determined in eggs from Reiaren. In particular PCB126 contributed highly to the total TEQ (69-82%). In 19 of the 23 samples the calculated WHO TEQ was higher than the TEQCALUX. Using CALUX specific relative effect potencies (REPs), the levels were lower at between 77 and 292 pg/g lw in eggs from Musvær and between 55 and 223 pg/g lw in eggs from Reiaren, which was higher than the TEQCALUX in 16 of the 23 samples. However, the means of the REP values and the TEQCALUX were not significantly different. This suggests the presence of compounds that can elicit antagonist effects, with a low binding affinity to the AhR. Non-target analysis identified the presence of hexachlorobenzene (HCB) (quantified at 9.6-185 pg/g lw) but neither this compound nor high concentrations of PCB126 and non-dioxin-like PCBs could explain the differences between the calculated TEQ or REP values and the TEQCALUX. Even though, for most AhR agonists, the sensitivity of herring gulls is not known, the reported levels can be considered to represent a risk for biological effects in the developing embryo, compared to LC50 values in chicken embryos. For human consumers of herring gull eggs, these eggs contain TEQ levels up to four times higher than the maximum tolerable weekly intake. Copyright © 2015 Elsevier B.V. All rights reserved.

  10. Haplosporidium nelsoni (MSX) in Japanese scallops Patinopecten yessoensis (Jay, 1857) from Dalian along the northern coast of the Yellow Sea, China.

    Science.gov (United States)

    Wang, Zhongwei; Lu, Xin; Liang, Yubo

    2012-04-01

    The protozoan parasite Haplosporidium nelsoni (MSX) was identified in Japanese scallops Patinopecten yessoensis (Jay, 1857) from Dalian along the northern coast of the Yellow Sea, China by histopathologic examination, polymerase chain reaction (PCR) amplification, and in situ hybridization (ISH) assay. H. nelsoni plasmodia-like structures were identified in the digestive glands of scallops by histologic examination, but no parasite spores were observed. PCR using the Hap-F2, R2 primer pair produced a sequence with 100% homology with the corresponding small subunit rDNA region of H. nelsoni. An ISH assay using the oligonucleotide probe MSX1347 produced a positive reaction with the Japanese scallop parasite. This is the first report of H. nelsoni in P. yessoensis in China.

  11. Spatial and temporal variation in winter condition of juvenile Pacific herring (Clupea pallasii) in Prince William Sound, Alaska: Oceanographic exchange with the Gulf of Alaska

    Science.gov (United States)

    Gorman, Kristen B.; Kline, Thomas C.; Roberts, Megan E.; Sewall, Fletcher F.; Heintz, Ron A.; Pegau, W. Scott

    2018-01-01

    Spatial variability in early and late winter measures of whole body energy density of juvenile (age-0) Pacific herring (Clupea pallasii) of Prince William Sound (PWS), Alaska was examined over nine years of study. Pacific herring in this region remain considered as an injured resource over the 25 years following the Exxon Valdez oil spill, however factors responsible for the lack of recovery by herring in PWS are a source of ongoing debate. Given the species' key ecological role in energy transfer to higher predators, and its economic role in a historical commercial fishery within the region, significant research effort has focused on understanding environmental factors that shape nutritional processes and the quality of these young forage fish. During November (early winter), factors such as juvenile herring body size, hydrological region of PWS, year, and the interaction between carbon (δ13C‧) or nitrogen (δ15N) stable isotope signature and hydrological region were all important predictors of juvenile herring energy density. In particular, analyses indicated that in the northern and western regions of PWS, juvenile herring with more depleted δ13C‧ values (which reflect a Gulf of Alaska carbon source) were more energy dense. Results suggest that intrusion of water derived from the Gulf of Alaska enhances the condition of age-0 herring possibly through alterations in zooplankton community structure and abundance, particularly in the northern and western regions of PWS in the fall, which is consistent with regional circulation. During March (late winter), factors such as juvenile herring body size, year, and the interaction between δ13C‧ or δ15N isotope signature and year were all important predictors of juvenile herring energy density. Results differed for early and late winter regarding the interaction between stable isotope signatures and region or year, suggesting important seasonal aspects of circulation contribute to variation in PWS juvenile

  12. Demographic processes underlying subtle patterns of population structure in the scalloped hammerhead shark, Sphyrna lewini.

    Directory of Open Access Journals (Sweden)

    Holly A Nance

    Full Text Available Genetic diversity (θ, effective population size (N(e, and contemporary levels of gene flow are important parameters to estimate for species of conservation concern, such as the globally endangered scalloped hammerhead shark, Sphyrna lewini. Therefore, we have reconstructed the demographic history of S. lewini across its Eastern Pacific (EP range by applying classical and coalescent population genetic methods to a combination of 15 microsatellite loci and mtDNA control region sequences. In addition to significant population genetic structure and isolation-by-distance among seven coastal sites between central Mexico and Ecuador, the analyses revealed that all populations have experienced a bottleneck and that all current values of θ are at least an order of magnitude smaller than ancestral θ, indicating large decreases in N(e (θ = 4N(eμ, where μ is the mutation rate. Application of the isolation-with-migration (IM model showed modest but significant genetic connectivity between most sampled sites (point estimates of Nm = 0.1-16.7, with divergence times (t among all populations significantly greater than zero. Using a conservative (i.e., slow fossil-based taxon-specific phylogenetic calibration for mtDNA mutation rates, posterior probability distributions (PPDs for the onset of the decline in N(e predate modern fishing in this region. The cause of decline over the last several thousand years is unknown but is highly atypical as a post-glacial demographic history. Regardless of the cause, our data and analyses suggest that S. lewini was far more abundant throughout the EP in the past than at present.

  13. Novel 14-nm Scallop-Shaped FinFETs (S-FinFETs) on Bulk-Si Substrate

    OpenAIRE

    Xu, Weijia; Yin, Huaxiang; Ma, Xiaolong; Hong, Peizhen; Xu, Miao; Meng, Lingkuan

    2015-01-01

    In this study, novel p-type scallop-shaped fin field-effect transistors (S-FinFETs) are fabricated using an all-last high-k/metal gate (HKMG) process on bulk-silicon (Si) substrates for the first time. In combination with the structure advantage of conventional Si nanowires, the proposed S-FinFETs provide better electrostatic integrity in the channels than normal bulk-Si FinFETs or tri-gate devices with rectangular or trapezoidal fins. It is due to formation of quasi-surrounding gate electrod...

  14. Spring molybdenum enrichment in scallop shells: a potential tracer of diatom productivity in temperate coastal environments (Brittany, NW France)

    Science.gov (United States)

    Barats, A.; Amouroux, D.; Pécheyran, C.; Chauvaud, L.; Thébault, J.; Donard, O. F. X.

    2010-01-01

    Skeletal molybdenum/calcium ([Mo]/[Ca])shell ratios were examined in shells of the Great Scallop Pecten maximus collected in temperate coastal environments of Western Europe (42 to 49° N). These ratios were determined by quantitative LA-ICP-MS analyses of daily striae taken every third day (i.e. a temporal resolution of 3 days) in 36 flat valves (2-years old; 3 shells/year). Variations of ([Mo]/[Ca])shell ratios were significant and reproducible for scallops from the same population, from different years (1998-2004) and temperate coastal locations (NW France). The [Mo]/[Ca])shell ratios exhibit typical profiles characterized by a background content, below the detection limit for this method (<0.003 μmol/mol) for most of the shell growth period, which is punctuated by a significant transient enrichment (0.031-2.1 μmol/mol) mainly occurring from May to June. The Bay of Brest (France) was investigated in particular because of its long term observations on scallop communities, environmental variables, and high resolution analyses of dissolved Mo in bottom seawater in 2000. In 2000, dissolved Mo exhibited a significant increase in concentration just preceding the maximum ([Mo]/[Ca])shell ratio. Both the intense monitoring survey in 2000 and over the 7-year period indicates that the ([Mo]/[Ca])shell maximum is directly influenced by spring changes of environmental conditions at the sediment water interface (SWI), occurring subsequent to the intense and periodic spring bloom. Spring maxima of ([Mo]/[Ca])shell ratios are closely correlated to the extent of silicic acid and nitrate depletion in seawater between winter and late spring (r2=0.878 and 0.780, p<0.05, n=6) that reflects diatom uptake and productivity in the Bay of Brest. The Mo inputs in bottom waters and subsequent shell enrichment are thus suggested to be directly or indirectly influenced by such biogenic material input at the SWI. The [Mo]/[Ca])shell records thus reveal unexpected biogeochemical cycles of

  15. Purification and characterization of cathepsin D from herring muscle ( Clupea harengus )

    DEFF Research Database (Denmark)

    Nielsen, L.B.; Nielsen, Henrik Hauch

    2001-01-01

    hamatus) and trout ovary (Oncorhynchus mykiss). Digestion of the P-chain of oxidized insulin resulted in preferential cleavage at Leu(15)-Tyr(16), (47%), Tyr(16)-Leu(17) (34%) and Ala(14)- Leu(15) (18%). Incubation with myofibrils from herring muscle at pH 4.23 showed that the enzyme mainly degraded......Cathepsin D was purified and concentrated 469-fold from a homogenate of Clupea harengus muscle. The purified enzyme is a monomer with a molecular weight of 38 000-39 000. It is inhibited by pepstatin and has optimal activity at pH 2.5 with hemoglobin as the substrate. The isoelectric point is at p...

  16. Forecasting fish stock dynamics under climate change: Baltic herring (Clupea harengus) as a case study

    DEFF Research Database (Denmark)

    Bartolino, V.; Margonski, P.; Lindegren, Martin

    2014-01-01

    Climate change and anthropogenic disturbances may affect marine populations and ecosystems through multiple pathways. In this study we present a framework in which we integrate existing models and knowledge on basic regulatory processes to investigate the potential impact of future scenarios...... of fisheries exploitation and climate change on the temporal dynamics of the central Baltic herring stock. Alternative scenarios of increasing sea surface temperature and decreasing salinity of the Baltic Sea from a global climate model were combined with two alternative fishing scenarios, and their direct......-term fish dynamics can be an informative tool to derive expectations of the potential long-term impact of alternative future scenarios of exploitation and climate change...

  17. The North Sea autumn spawning herring (Clupea harengus L.) Spawning Component Abundance Index (SCAI)

    DEFF Research Database (Denmark)

    2013-01-01

    , the sum of the fitted abundance indices across all components proves an excellent proxy for the biomass of the total stock, even though the model utilizes information at the individual-component level. The Orkney-Shetland component appears to have recovered faster from historic depletion events than......The North Sea autumn-spawning herring (Clupea harengus) stock consists of a set of different spawning components. The dynamics of the entire stock have been well characterized, but although time-series of larval abundance indices are available for the individual components, study of the dynamics...... at the component level has historically been hampered by missing observations and high sampling noise. A simple state-space statistical model is developed that is robust to these problems, gives a good fit to the data, and proves capable of both handling and predicting missing observations well. Furthermore...

  18. The effect of progressive hypoxia on school structure and dynamics in Atlantic herring Clupea harengus

    DEFF Research Database (Denmark)

    Domenici, Paolo; Ferrari, R Silvana; Steffensen, John F

    2002-01-01

    The effect of progressive hypoxia on the structure and dynamics of herring (Clupea harengus) schools in laboratory conditions was investigated. The length, width and depth of schools of about 20 individuals were measured from video recordings to test the hypothesis that during hypoxia fish schools...... change their shape and volume. School shape (calculated as the ratios of length/depth, width/depth and length/width) did not change significantly during hypoxia. School length, width, depth, area and volume were all significantly increased at 20% oxygen saturation. Volume, area and width were more...... to overtaking or falling back by individual fishes. School integrity and positional dynamics are the outcome of trade-offs among a number of biotic factors, such as food, predator defence, mating behaviour and various physical factors that may impose certain limits. Among these, our results indicate that oxygen...

  19. On the intraspecific variation in morphometry and shape of sagittal otoliths of common sardine, Strangomera bentincki, off central-southern Chile

    Directory of Open Access Journals (Sweden)

    Sandra Curin-Osorio

    2012-11-01

    Full Text Available Size and shape of fish otoliths are species-specific, but some species also display intraspecific variations. The common sardine, Strangomera bentincki, is a small pelagic fish inhabiting a seasonal upwelling ecosystem off central-southern Chile, having two discrete spawning sites along its latitudinal distribution. Otoliths of specimens were collected from commercial catches in Talcahuano and Corral, representing the central and south spawning zones. On the basis of otolith images, size-based shape descriptors were used to detect ontogenetic variation, and morphometric variables (length, breadth, area, perimeter and weight were used to detect geographical differences in size and shape of otoliths. Outline analysis was studied on the basis of elliptic Fourier descriptors through multivariate statistical procedures. Size-based shape descriptors showed that otolith shape starts to be stable for fish larger than 12 cm total length, which keep an elliptical form. Morphometric variables for fish larger than 12 cm revealed intraspecific variation between central and south zones, which were associated with otolith weight and breadth. Outline analysis did not reveal significant spatial differences, but extreme intraspecific variation was due to the antirostrum, excisure, and posterior part of otoliths. Intraspecific variation in otolith size could be linked to differences in each spawning habitat and related to geographical origin, whose differences are not clearly identified. It is concluded that intraspecific variability in morphometric variables of sardine otoliths revealed geographic differences in size that are not attributable to allometric effects, and that otolith shape was similar between specimens from different geographic origin.

  20. Preparation of triacylglycerols rich in omega-3 fatty acids from sardine oil using a Rhizomucor miehei lipase: focus in the EPA/DHA ratio.

    Science.gov (United States)

    Bispo, Paulo; Batista, Irineu; Bernardino, Raul J; Bandarra, Narcisa Maria

    2014-02-01

    The increasing evidence on the differential biochemical effects of eicosapentaenoic acid (EPA)/docosahexaenoic acid (DHA) raises the need of n-3 highly unsaturated fatty acid concentrates with different amounts of these fatty acids. In the present work, physicochemical and enzymatic techniques were combined to obtain acylglycerols, mainly triacylglycerols (TAG), rich in n-3 fatty acids. Sardine oil was obtained by washing sardine (Sardina pilchardus) mince with a NaHCO3 solution, hydrolyzed in a KOH-ethanol solution, and concentrated with urea. The esterification reaction was performed in the stoichiometric proportion of substrates for re-esterification to TAG, with 10 % level of Rhizomucor miehei lipase based on the weight of substrates, without any solvent, during 48 h. This procedure led to approximately 88 % of acylglycerols, where more than 66 % were TAG and the concentration of n-3 fatty acids was higher than 60 %, the EPA and DHA ratio (EPA/DHA) was 4:1. The content of DHA in the unesterifed fraction (free fatty acids) increased from 20 to 54 %, while the EPA level in the same fraction decreased from 33 to 12.5 % (EPA/DHA ratio ≈1:4). Computational methods (density functional theory calculations) have been carried out at the B3LYP/6-31G(d,p) level to explain some of the experimental results.

  1. 210Po and 210Pb trophic transfer within the phytoplankton-zooplankton-anchovy/sardine food web: a case study from the Gulf of Lion (NW Mediterranean Sea).

    Science.gov (United States)

    Strady, Emilie; Harmelin-Vivien, Mireille; Chiffoleau, Jean François; Veron, Alain; Tronczynski, Jacek; Radakovitch, Olivier

    2015-05-01

    The transfer of (210)Po and (210)Pb in the food web of small pelagic fishes (from phytoplankton and zooplankton to anchovy Engraulis encrasicolus and sardine Sardina pilchardus) is investigated in the Gulf of Lion (GoL). We present original data of (210)Po and (210)Pb activity concentrations, C and N stable isotope ratios, measured (i) from different size classes of phytoplankton and zooplankton during spring and winter in different environments of the GoL, and (ii) in two fish species. Significant spatial patterns based on (210)Po, (210)Pb activity concentrations and (210)Po/(210)Pb ratios in the different plankton size classes are evidenced by hierarchical clustering, both in spring and winter. This variability, also observed for C and N stable isotopes ratios, is connected to local specific pelagic habitats and hydrodynamics. The sampling strategy suggests that (210)Po bioaccumulation in the GoL remains at a constant level from the first (dominated by phytoplankton) to the second trophic level (zooplankton), while (210)Pb bioaccumulation shows an increase in winter. Based on stable N isotope ratios and (210)Po activity concentrations measured in anchovies and sardines, we evidence (210)Po bio-magnification along the trophic food web of these two planktivorous pelagic fishes. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Real-time ichthyoplankton drift in Northeast Arctic cod and Norwegian spring-spawning herring.

    Science.gov (United States)

    Vikebø, Frode B; Ådlandsvik, Bjørn; Albretsen, Jon; Sundby, Svein; Stenevik, Erling Kåre; Huse, Geir; Svendsen, Einar; Kristiansen, Trond; Eriksen, Elena

    2011-01-01

    Individual-based biophysical larval models, initialized and parameterized by observations, enable numerical investigations of various factors regulating survival of young fish until they recruit into the adult population. Exponentially decreasing numbers in Northeast Arctic cod and Norwegian Spring Spawning herring early changes emphasizes the importance of early life history, when ichthyoplankton exhibit pelagic free drift. However, while most studies are concerned with past recruitment variability it is also important to establish real-time predictions of ichthyoplankton distributions due to the increasing human activity in fish habitats and the need for distribution predictions that could potentially improve field coverage of ichthyoplankton. A system has been developed for operational simulation of ichthyoplankton distributions. We have coupled a two-day ocean forecasts from the Norwegian Meteorological Institute with an individual-based ichthyoplankton model for Northeast Arctic cod and Norwegian Spring Spawning herring producing daily updated maps of ichthyoplankton distributions. Recent years observed spawning distribution and intensity have been used as input to the model system. The system has been running in an operational mode since 2008. Surveys are expensive and distributions of early stages are therefore only covered once or twice a year. Comparison between model and observations are therefore limited in time. However, the observed and simulated distributions of juvenile fish tend to agree well during early fall. Area-overlap between modeled and observed juveniles September 1(st) range from 61 to 73%, and 61 to 71% when weighted by concentrations. The model system may be used to evaluate the design of ongoing surveys, to quantify the overlap with harmful substances in the ocean after accidental spills, as well as management planning of particular risky operations at sea. The modeled distributions are already utilized during research surveys to

  3. Real-time ichthyoplankton drift in Northeast Arctic cod and Norwegian spring-spawning herring.

    Directory of Open Access Journals (Sweden)

    Frode B Vikebø

    Full Text Available BACKGROUND: Individual-based biophysical larval models, initialized and parameterized by observations, enable numerical investigations of various factors regulating survival of young fish until they recruit into the adult population. Exponentially decreasing numbers in Northeast Arctic cod and Norwegian Spring Spawning herring early changes emphasizes the importance of early life history, when ichthyoplankton exhibit pelagic free drift. However, while most studies are concerned with past recruitment variability it is also important to establish real-time predictions of ichthyoplankton distributions due to the increasing human activity in fish habitats and the need for distribution predictions that could potentially improve field coverage of ichthyoplankton. METHODOLOGY/PRINCIPAL FINDINGS: A system has been developed for operational simulation of ichthyoplankton distributions. We have coupled a two-day ocean forecasts from the Norwegian Meteorological Institute with an individual-based ichthyoplankton model for Northeast Arctic cod and Norwegian Spring Spawning herring producing daily updated maps of ichthyoplankton distributions. Recent years observed spawning distribution and intensity have been used as input to the model system. The system has been running in an operational mode since 2008. Surveys are expensive and distributions of early stages are therefore only covered once or twice a year. Comparison between model and observations are therefore limited in time. However, the observed and simulated distributions of juvenile fish tend to agree well during early fall. Area-overlap between modeled and observed juveniles September 1(st range from 61 to 73%, and 61 to 71% when weighted by concentrations. CONCLUSIONS/SIGNIFICANCE: The model system may be used to evaluate the design of ongoing surveys, to quantify the overlap with harmful substances in the ocean after accidental spills, as well as management planning of particular risky operations

  4. Seasonal dynamics of Atlantic herring (Clupea harengus L.) populations spawning in the vicinity of marginal habitats.

    Science.gov (United States)

    Eggers, Florian; Slotte, Aril; Libungan, Lísa Anne; Johannessen, Arne; Kvamme, Cecilie; Moland, Even; Olsen, Esben M; Nash, Richard D M

    2014-01-01

    Gillnet sampling and analyses of otolith shape, vertebral count and growth indicated the presence of three putative Atlantic herring (Clupea harengus L.) populations mixing together over the spawning season February-June inside and outside an inland brackish water lake (Landvikvannet) in southern Norway. Peak spawning of oceanic Norwegian spring spawners and coastal Skagerrak spring spawners occurred in March-April with small proportions of spawners entering the lake. In comparison, spawning of Landvik herring peaked in May-June with high proportions found inside the lake, which could be explained by local adaptations to the environmental conditions and seasonal changes of this marginal habitat. The 1.85 km(2) lake was characterized by oxygen depletion occurring between 2.5 and 5 m depth between March and June. This was followed by changes in salinity from 1-7‰ in the 0-1 m surface layer to levels of 20-25‰ deeper than 10 m. In comparison, outside the 3 km long narrow channel connecting the lake with the neighboring fjord, no anoxic conditions were found. Here salinity in the surface layer increased over the season from 10 to 25‰, whereas deeper than 5 m it was stable at around 35‰. Temperature at 0-5 m depth increased significantly over the season in both habitats, from 7 to 14 °C outside and 5 to 17 °C inside the lake. Despite differences in peak spawning and utilization of the lake habitat between the three putative populations, there was an apparent temporal and spatial overlap in spawning stages suggesting potential interbreeding in accordance with the metapopulation concept.

  5. Seasonal dynamics of Atlantic herring (Clupea harengus L. populations spawning in the vicinity of marginal habitats.

    Directory of Open Access Journals (Sweden)

    Florian Eggers

    Full Text Available Gillnet sampling and analyses of otolith shape, vertebral count and growth indicated the presence of three putative Atlantic herring (Clupea harengus L. populations mixing together over the spawning season February-June inside and outside an inland brackish water lake (Landvikvannet in southern Norway. Peak spawning of oceanic Norwegian spring spawners and coastal Skagerrak spring spawners occurred in March-April with small proportions of spawners entering the lake. In comparison, spawning of Landvik herring peaked in May-June with high proportions found inside the lake, which could be explained by local adaptations to the environmental conditions and seasonal changes of this marginal habitat. The 1.85 km(2 lake was characterized by oxygen depletion occurring between 2.5 and 5 m depth between March and June. This was followed by changes in salinity from 1-7‰ in the 0-1 m surface layer to levels of 20-25‰ deeper than 10 m. In comparison, outside the 3 km long narrow channel connecting the lake with the neighboring fjord, no anoxic conditions were found. Here salinity in the surface layer increased over the season from 10 to 25‰, whereas deeper than 5 m it was stable at around 35‰. Temperature at 0-5 m depth increased significantly over the season in both habitats, from 7 to 14 °C outside and 5 to 17 °C inside the lake. Despite differences in peak spawning and utilization of the lake habitat between the three putative populations, there was an apparent temporal and spatial overlap in spawning stages suggesting potential interbreeding in accordance with the metapopulation concept.

  6. REDD herring

    DEFF Research Database (Denmark)

    Kamelarczyk, Kewin Bach Friis; Smith-Hall, Carsten

    2014-01-01

    To enhance understanding of environmental science–policy interactions, this study analyses how environmental knowledge is produced, circulated, and applied in the Reduced Emissions from Deforestation and Degradation (REDD +) programme in Zambia. Data are drawn from interviews with key actors...... in the REDD + process and an extensive critical review of policy documents and deforestation estimates. We find that research over the past 50 years has not resulted in accurate estimates of forest cover and deforestation rates, nor have major deforestation drivers been convincingly documented. Estimates...... are difficult to compare due to inconsistent use of key terms, methodological pluralism and differences in social framing. We argue that an epistemic community is able to influence production, circulation, and application of deforestation related knowledge. Furthermore, in a situation of weak and contradictory...

  7. Optimization of supercritical carbon dioxide (CO2 extraction of sardine (Sardinella lemuru Bleeker oil using response surface methodology (RSM

    Directory of Open Access Journals (Sweden)

    Gedi, M. A.

    2015-06-01

    Full Text Available Oil was extracted from freeze-dried sardine (Sardinella lemur fillets using supercritical carbon dioxide (SC-CO2 and a few milliliters of ethanol were optimized with response surface methodology (RSM. The impact of extraction pressure (200–400 bars and temperature (40–70 °C were studied on the total extraction yields, ratios of Eicosapentaenoic acid (EPA and Docosahexaenoic acid (DHA. The results were compared with those of Soxhlet and modified Kinsella methods (MKM. The oils obtained using the SC-CO2 and MKM methods were significantly (P El aceite se extrae de filetes de sardinas (Sardinella lemur liofilizando, mediante dióxido de carbono supercrítico (SC-CO2 y unos mililitros de etanol, optimizándose mediante la metodología de superficie de respuesta (RSM. Se ha estudiado la influencia de la presión de extracción (200–400 bars y la temperatura (40–70 °C sobre los rendimientos de extracción total, y sobre las relaciones de ácido eicosapentaenoico (EPA y ácido docosahexaenoico (DHA. Los resultados se compararon con los obtenidos mediante extracción con Soxhlet y el método de Kinsella modificado (MKM. Los aceites obtenidos mediante SC-CO2 y métodos MKM fueron significativamente (P < 0.05 superiores en rendimientos de aceite (8,04% y 6,83%, EPA (5,43% y 5,45% y DHA (18,76% y 18,54%, respectivamente, en comparación con rendimientos mediante Soxhlet (5,10%, EPA (2,17% y DHA (06,46%. De las dos variables independientes, la presión tuvo un efecto crítico sobre el rendimiento, mientras que los porcentajes de EPA y DHA estuvieron notablemente influenciados por la temperatura. Los valores óptimos fueron para una presión de 328 bar y una temperatura de 40 °C, y sus correspondientes respuestas fueron 7,20%, 5,68% y 20,09% para el rendimiento, EPA y DHA, respectivamente. Los valores experimentales de este estudio fueron los previstos y son comparables razonablemente con sus homólogos.

  8. Elimination of vibrio cholerae El Tor, in scallops (Argopecten purpuratus) by gamma radiation and product organoleptic evaluation

    International Nuclear Information System (INIS)

    Torres, Z.; Bernuy, B.; Vivanco, M.; Kahn, G.

    1999-03-01

    V. cholerae D 10 value in scallops (Argopecten purpuratus) was determined in vivo. D 10 was found to be 0,143 kGy, requiring therefore the application of 8D in scallops, equivalent to a 1,14 kGy dose, the optimal dose for life span extension of samples kept under refrigeration conditions (0-1 o ), and examined periodically under different analytic method criteria. Life span for the appearance characteristic reaches the acceptability limit of 3, after 11 days for control samples, and 16 and 13 days for samples radiated at 1 and 2 kGy. Smell of control samples was accepted only until the 13 th day while samples radiated at 1 and 2 kGy went beyond this level, reaching 19 and 17 days respectively. In the same way, life span for the flavor characteristic was extended to 19 and 20 days for samples radiated at 1 and 2 kGy, respectively, while control samples only reached 15 days. Control sample texture remained within acceptable limits until the 18 th day, whereas samples radiated at 1 and 2 kGy reached 21 and 17 days, respectively. Use of ph and nitrogen volatile bases were also evaluated as quality indicators. (authors)

  9. Accumulation of nine metals and one metalloid in the tropical scallop Comptopallium radula from coral reefs in New Caledonia

    Energy Technology Data Exchange (ETDEWEB)

    Metian, M. [International Atomic Energy Agency - Marine Environment Laboratories, 4 Quai Antoine Ier, MC-98000 Monaco (Monaco); Centre de Recherche sur les Ecosystemes Littoraux Anthropises, UMR 6217, CNRS-IFREMER-Universite de La Rochelle, 22 avenue Michel Crepeau, F-17042 La Rochelle Cedex 01 (France)], E-mail: mametian@gmail.com; Bustamante, P. [Centre de Recherche sur les Ecosystemes Littoraux Anthropises, UMR 6217, CNRS-IFREMER-Universite de La Rochelle, 22 avenue Michel Crepeau, F-17042 La Rochelle Cedex 01 (France)], E-mail: pbustama@univ-lr.fr; Hedouin, L. [International Atomic Energy Agency - Marine Environment Laboratories, 4 Quai Antoine Ier, MC-98000 Monaco (Monaco) and Centre de Recherche sur les Ecosystemes Littoraux Anthropises, UMR 6217, CNRS-IFREMER-Universite de La Rochelle, 22 avenue Michel Crepeau, F-17042 La Rochelle Cedex 01 (France) and IRD-Centre de Noumea, BP A5, 98848 Noumea Cedex, New Caledonia; Warnau, M. [International Atomic Energy Agency - Marine Environment Laboratories, 4 Quai Antoine Ier, MC-98000 Monaco (Monaco)], E-mail: m.warnau@iaea.org

    2008-04-15

    Uptake of waterborne Cd, Co, Mn and Zn was determined in laboratory experiments using radiotracer techniques ({sup 109}Cd, {sup 57}Co, {sup 54}Mn and {sup 65}Zn). Labelled Zn was mainly accumulated in the digestive gland (65%) and Co in kidneys (81%); Cd and Mn were similarly distributed in digestive gland and gills. In a complementary field study, Ag, As, Cd, Co, Cr, Cu, Fe, Mn, Ni, and Zn were analysed in scallops collected at two stations showing different contamination levels. Digestive gland and kidneys displayed the highest concentrations. Ag, As, Cd, and Fe differed in soft tissues from the two stations, suggesting that Comptopallium radula could be a valuable local biomonitor species for these elements. Low Mn and Zn concentrations found in kidneys suggest that their content in calcium-phosphate concretions differs from the other pectinids. Preliminary risk considerations suggest that As would be the only element potentially leading to exposure of concern for seafood consumers. - This study investigates metal accumulation behaviour in the tropical scallop Comptopallium radula and preliminary risk assessment for consumers.

  10. Characteristic antioxidant activity and comprehensive flavor compound profile of scallop (Chlamys farreri) mantle hydrolysates-ribose Maillard reaction products.

    Science.gov (United States)

    Han, Jia-Run; Yan, Jia-Nan; Sun, Shi-Guang; Tang, Yue; Shang, Wen-Hui; Li, Ao-Ting; Guo, Xiao-Kun; Du, Yi-Nan; Wu, Hai-Tao; Zhu, Bei-Wei; Xiong, Youling L

    2018-09-30

    The objective of the present study was to improve the utilization of scallop (Chlamys farreri) byproducts by using Maillard reaction. Scallop mantle hydrolysates (SMHs) were prepared using neutrase then reacted with ribose. Thirty-four peptides were identified from SMHs by UPLC-Q-TOF-MS, and the abundance of Asp and Lys suggested the strong Maillard reactivity. The formation of Schiff's base as well as modification of amide I, II and III bands in Maillard reaction products (MRPs) was confirmed by ultraviolet-visible, fluorescence, and Fourier transform infrared spectroscopy. Thirty volatile compounds were produced by the reaction of SMHs with ribose. Moreover, MRPs with enhanced radical scavenging and anti-linoleic acid peroxidation activities over SMHs promoted the survival and reduced the DNA damage of HepG2 cells treated with hydrogen peroxide. These results suggest that SMHs-ribose MRPs can be potentially used as food antioxidant for suppressing of lipid oxidation or protecting of cell from oxidative damage. Copyright © 2018 Elsevier Ltd. All rights reserved.

  11. THE GOAL AND THE GOLD MINE: Constraints Management and the Dutch Herring Fishing Industry, 1400-1700

    Directory of Open Access Journals (Sweden)

    Michael Scott Martin

    2009-01-01

    Full Text Available In many ways, the Netherlands set the pace for European economic growth in the fifteenth through the eighteenth centuries. The Dutch herring fishing industry was critical to this modern economic progress. A comparative study of this enterprise to the lean manufacturing tool of constraints management provides insight into how the Dutch dominated this lucrative industry. Improvements by Dutch fishing interests in technology, practice, procedures and quality control were consistent with the constraints management model. English and Scottish competitors failed to identify these constraints and thus, failed to challenge Dutch operators. The Dutch fishing industry practiced and understood constraints management and used it to monopolize the export herring market for three centuries.

  12. Mercury levels in herring gulls and fish: 42 years of spatio-temporal trends in the Great Lakes.

    Science.gov (United States)

    Blukacz-Richards, E Agnes; Visha, Ariola; Graham, Matthew L; McGoldrick, Daryl L; de Solla, Shane R; Moore, David J; Arhonditsis, George B

    2017-04-01

    Total mercury levels in aquatic birds and fish communities have been monitored across the Canadian Great Lakes by Environment and Climate Change Canada (ECCC) for the past 42 years (1974-2015). These data (22 sites) were used to examine spatio-temporal variability of mercury levels in herring gull (Larus argentatus) eggs, lake trout (Salvelinus namaycush), walleye (Sander vitreus), and rainbow smelt (Osmerus mordax). Trends were quantified with dynamic linear models, which provided time-variant rates of change of mercury concentrations. Lipid content (in both fish and eggs) and length in fish were used as covariates in all models. For the first three decades, mercury levels in gull eggs and fish declined at all stations. In the 2000s, trends for herring gull eggs reversed at two sites in Lake Erie and two sites in Lake Ontario. Similar trend reversals in the 2000s were observed for lake trout in Lake Superior and at a single station in Lake Ontario. Mercury levels in lake trout continued to slowly decline at all of the remaining stations, except for Lake Huron, where the levels remained stable. A post-hoc Bayesian regression analysis suggests strong trophic interactions between herring gulls and rainbow smelt in Lake Superior and Lake Ontario, but also pinpoints the likelihood of a trophic decoupling in Lake Huron and Lake Erie. Continued monitoring of mercury levels in herring gulls and fish is required to consolidate these trophic shifts and further evaluate their broader implications. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

  13. Principles underlying the epizootiology of viral hemorrhagic septicemia in Pacific herring and other fishes throughout the North Pacific Ocean

    Science.gov (United States)

    Hershberger, Paul K.; Garver, Kyle A.; Winton, James R.

    2016-01-01

    Although viral hemorrhagic septicemia virus (VHSV) typically occurs at low prevalence and intensity in natural populations of Pacific herring (Clupea pallasii) and other marine fishes in the Northeast Pacific Ocean, epizootics of the resulting disease (VHS) periodically occur, often in association with observed fish kills. Here we identify a list of principles, based on a combination of field studies, controlled laboratory experiments, and previously unpublished observations, that govern the epizootiology of VHS in Pacific herring. A thorough understanding of these principles provides the basis for identifying risk factors that predispose certain marine fish populations to VHS epizootics, including the lack of population resistance, presence of chronic viral carriers in a population, copious viral shedding by infected individuals, cool water temperatures, limited water circulation patterns, and gregarious host behavioral patterns. Further, these principles are used to define the epizootiological stages of the disease in Pacific herring, including the susceptible (where susceptible individuals predominate a school or subpopulation), enzootic (where infection prevalence and intensity are often below the limits of reasonable laboratory detection), disease amplification (where infection prevalence and intensity increase rapidly), outbreak (often accompanied by host mortalities with high virus loads and active shedding), recovery (in which the mortality rate and virus load decline owing to an active host immune response), and refractory stages (characterized by little or no susceptibility and where viral clearance occurs in most VHS survivors). In addition to providing a foundation for quantitatively assessing the potential risks of future VHS epizootics in Pacific herring, these principles provide insights into the epizootiology of VHS in other fish communities where susceptible species exist.

  14. Extensive use of mesopelagic waters by a Scalloped hammerhead shark (Sphyrna lewini) in the Red Sea

    KAUST Repository

    Spaet, Julia L.Y.

    2017-09-06

    Background Despite being frequently landed in fish markets along the Saudi Arabian Red Sea coast, information regarding fundamental biology of the Scalloped hammerhead shark (Sphyrna lewini) in this region is scarce. Satellite telemetry studies can generate important data on life history, describe critical habitats, and ultimately redefine management strategies for sharks. To better understand the horizontal and vertical habitat use of S. lewini in the Red Sea and to aid with potential future development of zoning and management plans for key habitats, we deployed a pop-up satellite archival transmitting tag to track a single female specimen (240 cm total length) for a tracking period of 182 days. Results The tag was physically recovered after a deployment period of 6 months, thus providing the complete archived dataset of more than one million depth and temperature records. Based on a reconstructed, most probable track, the shark travelled a circular distance of approximately 1000 km from the central Saudi Arabian Red Sea southeastward into Sudanese waters, returning to the tagging location toward the end of the tracking period. Mesopelagic excursions to depths between 650 and 971 m occurred on 174 of the 182 days of the tracking period. Intervals between such excursions were characterized by constant oscillatory diving in the upper 100 m of the water column. Conclusions This study provides evidence that mesopelagic habitats might be more commonly used by S. lewini than previously suggested. We identified deep diving behavior throughout the 24-h cycle over the entire 6-month tracking period. In addition to expected nightly vertical habitat use, the shark exhibited frequent mesopelagic excursions during daytime. Deep diving throughout the diel cycle has not been reported before and, while dive functionality remains unconfirmed, our study suggests that mesopelagic excursions may represent foraging events within and below deep scattering layers. Additional research

  15. Accumulation, Biotransformation, Histopathology and Paralysis in the Pacific Calico Scallop Argopecten ventricosus by the Paralyzing Toxins of the Dinoflagellate Gymnodinium catenatum

    Directory of Open Access Journals (Sweden)

    Rosalba Alonso-Rodriguez

    2012-05-01

    Full Text Available The dinoflagellate Gymnodinium catenatum produces paralyzing shellfish poisons that are consumed and accumulated by bivalves. We performed short-term feeding experiments to examine ingestion, accumulation, biotransformation, histopathology, and paralysis in the juvenile Pacific calico scallop Argopecten ventricosus that consume this dinoflagellate. Depletion of algal cells was measured in closed systems. Histopathological preparations were microscopically analyzed. Paralysis was observed and the time of recovery recorded. Accumulation and possible biotransformation of toxins were measured by HPLC analysis. Feeding activity in treated scallops showed that scallops produced pseudofeces, ingestion rates decreased at 8 h; approximately 60% of the scallops were paralyzed and melanin production and hemocyte aggregation were observed in several tissues at 15 h. HPLC analysis showed that the only toxins present in the dinoflagellates and scallops were the N-sulfo-carbamoyl toxins (C1, C2; after hydrolysis, the carbamate toxins (epimers GTX2/3 were present. C1 and C2 toxins were most common in the mantle, followed by the digestive gland and stomach-complex, adductor muscle, kidney and rectum group, and finally, gills. Toxin profiles in scallop tissue were similar to the dinoflagellate; biotransformations were not present in the scallops in this short-term feeding experiment.

  16. In situ exposure of herring embryos in Prince William Sound two years after the Exxon Valdez oil spill

    International Nuclear Information System (INIS)

    Kocan, R.; Brown, E.; Baker, T.

    1995-01-01

    In order to evaluate long-term residual toxicity, artificially spawned Pacific herring (Clupea pallasl) embryos were deployed at 5 oiled and 5 unoiled sites in Prince William Sound two years after the Exxon Valdez oil spill. Embryos were exposed at 1.5 m and 4.5 m below mean low water for 8--10 d post fertilization. The embryos were then retrieved and transported to the laboratory for hatching and evaluation under controlled conditions, Endpoints were (1) embryo survival, (2) live hatch, (3) deformed larvae and (4) larval dry weight. The oiled sites produced significantly (P < 0.01) more deformed larvae (63.3%) than did the unoiled sites (51.3%), but there was a lower hatching success at the unoiled sites which resulted in no overall difference in normal live larvae produced between oiled and unoiled sites. The mean dry weight of newly hatched larvae from the oiled sites (78 microg/larva) was significantly lower than those from the unoiled sites (97 microg/larva) at all depths (P < 0.01). Increased larval deformities and reduced hatching weight is consistent with what has been reported by several investigators for herring larvae experimentally and naturally exposed to petroleum hydrocarbons. Based on the data, there appeared to be differences between the previously oiled and unoiled sites relative to herring development, but it is not clear whether this was due to residual oil effects or parental effects

  17. Round herring (genus Etrumeus) contain distinct evolutionary lineages coincident with a biogeographic barrier along Australia’s southern temperate coastline

    KAUST Repository

    DiBattista, Joseph; Randall, John E.; Newman, Stephen J.; Bowen, Brian W.

    2014-01-01

    Molecular genetic surveys of marine fishes have revealed that some widely distributed species are actually a composite of multiple evolutionary lineages. This is apparent in the round herrings (genus Etrumeus), wherein a globally distributed taxon (Etrumeus sadina Mitchill 1814) has proven to contain at least seven valid taxa, with more likely awaiting discovery. Here, we survey evolutionary lineages of the nominal E. sadina (formerly E. teres, a junior synonym) across the southern temperate zone of Australia, a marine region divided into three biogeographic provinces based primarily on the distribution of intertidal faunas. Results from morphological and mitochondrial DNA data reveal two evolutionary lineages corresponding to eastern and southwestern provinces (d = 0.007 for cytochrome c oxidase subunit I and d = 0.017 for cytochrome b), possibly initiated by the Bassian Isthmus between Australia and Tasmania during low sea-level stands. The Australian round herring is also genetically distinct from the nearest congeneric forms in the Indian and Pacific Oceans, with a corresponding modal difference in gill-raker counts in most cases. Based on these data, we resurrect the title Etrumeus jacksoniensis for the Australian round herring. While the Bassian Isthmus may have initiated the partition of evolutionary lineages within Australia, additional oceanographic and ecological factors must reinforce this separation in order to maintain diagnostic genetic differences along a continuous temperate coastline. © 2014 Springer-Verlag Berlin Heidelberg.

  18. Round herring (genus Etrumeus) contain distinct evolutionary lineages coincident with a biogeographic barrier along Australia’s southern temperate coastline

    KAUST Repository

    DiBattista, Joseph

    2014-08-28

    Molecular genetic surveys of marine fishes have revealed that some widely distributed species are actually a composite of multiple evolutionary lineages. This is apparent in the round herrings (genus Etrumeus), wherein a globally distributed taxon (Etrumeus sadina Mitchill 1814) has proven to contain at least seven valid taxa, with more likely awaiting discovery. Here, we survey evolutionary lineages of the nominal E. sadina (formerly E. teres, a junior synonym) across the southern temperate zone of Australia, a marine region divided into three biogeographic provinces based primarily on the distribution of intertidal faunas. Results from morphological and mitochondrial DNA data reveal two evolutionary lineages corresponding to eastern and southwestern provinces (d = 0.007 for cytochrome c oxidase subunit I and d = 0.017 for cytochrome b), possibly initiated by the Bassian Isthmus between Australia and Tasmania during low sea-level stands. The Australian round herring is also genetically distinct from the nearest congeneric forms in the Indian and Pacific Oceans, with a corresponding modal difference in gill-raker counts in most cases. Based on these data, we resurrect the title Etrumeus jacksoniensis for the Australian round herring. While the Bassian Isthmus may have initiated the partition of evolutionary lineages within Australia, additional oceanographic and ecological factors must reinforce this separation in order to maintain diagnostic genetic differences along a continuous temperate coastline. © 2014 Springer-Verlag Berlin Heidelberg.

  19. Muscle Thiobarbituric Acid Reactive Substance of the Atlantic Herring (Clupea harengus in Marinades Collected in the Market Network

    Directory of Open Access Journals (Sweden)

    Alena Halamíčková

    2010-01-01

    Full Text Available Fish fat belongs to highly specific nutritious elements especially due to its high content of polyunsaturated fatty acids. The aim of the present study was to determine the content of 2-thiobarbituric acid reactive substances in over-the-counter cold and warm marinades in which the base fish material consists of the Atlantic herring (Clupea harengus. Fifty six marinated fish products stored at refrigeration temperatures were analyzed before their expiration date. Their fat content was determined by means of the Soxhlet method and the TBARS validated fluorimetric micromethod. Lowest TBARS values were characteristic of warm baked marinades (1.17 ± 0.40 mg MDA/ kg muscle and in case of cold marinades for the rolled herring fillets with pepper (5.03 ± 0.54 mg MDA/kg muscle whereas the highest TBARS values were observed in warm cooked marinades (16.48 ± 4.22 mg MDA/kg muscle and in roll mops sold over the counter (7.61 ± 3.87 mg MDA/kg muscle. The results showed that fat content is not always critical for herring TBARS determination in marinades. For the consumer safety it is essential to pay attention to cold marinades in brine sold over the counter and baked marinades in aspic before their expiration date.

  20. Characteristics of the Norwegian Coastal Current during Years with High Recruitment of Norwegian Spring Spawning Herring (Clupea harengus L..

    Directory of Open Access Journals (Sweden)

    Øystein Skagseth

    Full Text Available Norwegian Spring Spawning herring (NSSH Clupea harengus L. spawn on coastal banks along the west coast of Norway. The larvae are generally transported northward in the Norwegian Coastal Current (NCC with many individuals utilizing nursery grounds in the Barents Sea. The recruitment to this stock is highly variable with a few years having exceptionally good recruitment. The principal causes of recruitment variability of this herring population have been elusive. Here we undertake an event analysis using data between 1948 and 2010 to gain insight into the physical conditions in the NCC that coincide with years of high recruitment. In contrast to a typical year when northerly upwelling winds are prominent during spring, the years with high recruitment coincide with predominantly southwesterly winds and weak upwelling in spring and summer, which lead to an enhanced northward coastal current during the larval drift period. Also in most peak recruitment years, low-salinity anomalies are observed to propagate northward during the spring and summer. It is suggested that consistent southwesterly (downwelling winds and propagating low-salinity anomalies, both leading to an enhanced northward transport of larvae, are important factors for elevated recruitment. At the same time, these conditions stabilize the coastal waters, possibly leading to enhanced production and improved feeding potential along the drift route to Barents Sea. Further studies on the drivers of early life history mortality can now be undertaken with a better understanding of the physical conditions that prevail during years when elevated recruitment occurs in this herring stock.

  1. SOME BIOLOGICAL ASPECTS OF SCALLOPED HAMMERHEAD SHARKS (Sphyrna lewini Griffith & Smith, 1834 CAUGHT FROM COASTAL FISHERIES IN THE EASTERN INDIAN OCEAN

    Directory of Open Access Journals (Sweden)

    Umi Chodrijah

    2015-12-01

    Full Text Available Indonesia has the largest chondrichthyan fishery in the world, with a reported of 105,000 and 118,000 tonnes landed in 2002 and 2003 respectively. Scalloped hammerhead shark was either targeted or by-catch from this fishery, mostly for its fins. Despite of the growing concern around the world, the availability of biological data of this species, especially in the Eastern Indian Ocean is still lacking. The objectives of this paper are to present some biological information (size composition and sex ratio of the scalloped hammerhead, from coastal fisheries in Eastern Indian Ocean. The data used for the analysis comprised of two components, i.e. survey data in 2010 (February, March, June, August, October and December and data from daily monitoring shark landing in 2013 (January to December. Substantially lower mean size, more immature sharks and more frequent of female caught over years showed that scalloped hammerhead shark in the Eastern Indian Ocean are facing intensive fishing pressure which could lead to overfishing. This could harm the sustainability of scalloped hammerhead shark resource in the long run. The relationship between clasper length and total length was positively correlated where every 5 cmTL increment on clasper length adding 51 cmTL on total length.

  2. Accumulation, Biotransformation, Histopathology and Paralysis in the Pacific Calico Scallop Argopecten ventricosus by the Paralyzing Toxins of the Dinoflagellate Gymnodinium catenatum

    OpenAIRE

    Escobedo-Lozano, Amada Y.; Estrada, Norma; Ascencio, Felipe; Contreras, Gerardo; Alonso-Rodriguez, Rosalba

    2012-01-01

    The dinoflagellate Gymnodinium catenatum produces paralyzing shellfish poisons that are consumed and accumulated by bivalves. We performed short-term feeding experiments to examine ingestion, accumulation, biotransformation, histopathology, and paralysis in the juvenile Pacific calico scallop Argopecten ventricosus that consume this dinoflagellate. Depletion of algal cells was measured in closed systems. Histopathological preparations were microscopic...

  3. Functional and antioxidant properties of hydrolysates of sardine (S. pilchardus) and horse mackerel (T. mediterraneus) for the microencapsulation of fish oil by spray-drying.

    Science.gov (United States)

    Morales-Medina, R; Tamm, F; Guadix, A M; Guadix, E M; Drusch, S

    2016-03-01

    The functionality of fish protein hydrolysates (FPH) for the microencapsulation of fish oil was investigated. Muscle protein from sardine (Sardina pilchardus) and horse mackerel (Trachurus mediterraneus) was hydrolysed using Alcalase or trypsin. Physically stable emulsions suitable for spray-drying were obtained when using FPH with a degree of hydrolysis of 5%. Microencapsulation efficiency amounted to 98±0.1% and oxidative stability of the encapsulated oil over a period of twelve weeks was in a similar range as it is reported for other matrix systems. Therefore, the suitability of FPH for use in spray-dried emulsions has been shown for the first time. Since no clear correlation between the antioxidative activity of the FPH and the course of lipid oxidation could be established future research is required to more specifically characterise the molecular structure of the peptides and its impact on protein alteration and role in lipid oxidation. Copyright © 2015 Elsevier Ltd. All rights reserved.

  4. Monitoring the sardine (Sardinella brasiliensis fermentation process to obtain anchovies Monitoramento do processo de fermentação da sardinha, Sardinella brasiliensis, para obtenção de anchovas

    Directory of Open Access Journals (Sweden)

    Marília Oetterer

    2003-01-01

    Full Text Available Anchovies are traditional fish preserves, prepared from fermented fish of the engraulidae family, mainly in European countries. In Brazil, sardines (Sardinella brasiliensis are an alternative fish for preparing these types of preserves, provided that the preservation process results in a high quality product. In this research, sardines were prepared for preservation and physicochemical, microbiological and sensory analyses were carried out during the preservation process. Whole or eviscerated sardines, with or without condiments/preservatives and with 20% of salt (w/w were used. Sardines were analyzed fresh, and at 1, 15, 30, 45 and 60 days along the preservation process. The use of whole sardines, with or without condiments/preservatives, presented best results, with increased non-proteic nitrogen in the dry matter, higher levels of total volatile bases and higher contents of lactic acid and sodium chloride. The higher acidity observed in the whole sardine treatments resulted in better control of halophylic mesophilic microorganisms, which were kept under 1.4 x 10³ CFU g-1 in both treatments. Total coliforms and Staphylococcus aureus reached 21 and 3.0 x 10² CFU g-1, respectively. Escherichia coli and Salmonella spp were not present in the fresh sardines or in any of the four treatments, indicating that the concentration of salt used was appropriate to maintain the product under adequate microbiological control. Both whole or eviscerated sardines under the conditions of this experiment were appropriate in terms of the microbiological safety of the preserves. Treatments using whole fish, either with or without condiments/preservatives, also presented better sensorial properties such as color, flavor, taste and texture, as compared to the eviscerated fish treatments. Whole sardines produced good quality, anchovy-type preserves, which can be used for consumption and marketing purposes.As sardinhas brasileiras podem ser utilizadas para o preparo

  5. Sardine protein diet increases plasma glucagon-like peptide-1 levels and prevents tissue oxidative stress in rats fed a high-fructose diet.

    Science.gov (United States)

    Madani, Zohra; Sener, Abdullah; Malaisse, Willy J; Dalila, Ait Yahia

    2015-11-01

    The current study investigated whether sardine protein mitigates the adverse effects of fructose on plasma glucagon‑like peptide-1 (GLP-1) and oxidative stress in rats. Rats were fed casein (C) or sardine protein (S) with or without high‑fructose (HF) for 2 months. Plasma glucose, insulin, GLP‑1, lipid and protein oxidation and antioxidant enzymes were assayed. HF rats developed obesity, hyperglycemia, hyperinsulinemia, insulin resistance and oxidative stress despite reduced energy and food intakes. High plasma creatinine and uric acid levels, in addition to albuminuria were observed in the HF groups. The S‑HF diet reduced plasma glucose, insulin, creatinine, uric acid and homeostasis model assessment‑insulin resistance index levels, however increased GLP‑1 levels compared with the C‑HF diet. Hydroperoxides were reduced in the liver, kidney, heart and muscle of S‑HF fed rats compared with C‑HF fed rats. A reduction in liver, kidney and heart carbonyls was observed in S‑HF fed rats compared with C‑HF fed rats. Reduced levels of nitric oxide (NO) were detected in the liver, kidney and heart of the S‑HF fed rats compared with C‑HF fed rats. The S diet compared with the C diet reduced levels of liver hydroperoxides, heart carbonyls and kidney NO. The S‑HF diet compared with the C‑HF diet increased the levels of liver and kidney superoxide dismutase, liver and muscle catalase, liver, heart and muscle glutathione peroxidase and liver ascorbic acid. The S diet prevented and reversed insulin resistance and oxidative stress, and may have benefits in patients with metabolic syndrome.

  6. Viral replication in excised fin tissues (VREFT) corresponds with prior exposure of Pacific herring, Clupea pallasii (Valenciennes), to viral haemorrhagic septicaemia virus (VHSV)

    Science.gov (United States)

    Grady, C.A.; Gregg, J.L.; Wade, R.M.; Winton, J.R.; Hershberger, P.K.

    2011-01-01

    Procedures for a viral replication in excised fin tissue (VREFT) assay were adapted to Pacific herring, Clupea pallasii, and optimized both to reduce processing time and to provide the greatest resolution between na??ve herring and those previously exposed to viral haemorrhagic septicaemia virus (VHSV), Genogroup IVa. The optimized procedures included removal of the left pectoral fin from a euthanized fish, inoculation of the fin with >105 plaque-forming units (PFU) mL-1 VHSV for 1 h, rinsing the fin in fresh medium six times to remove unadsorbed virions, incubation of the fin in fresh medium for 4 days and enumeration of the viral titre in a sample of the incubation medium by plaque assay. The optimized VREFT assay was effective at identifying the prior exposure history of laboratory-reared Pacific herring to VHSV. The geometric mean VREFT value was significantly greater (P < 0.01) among na??ve herring (1.2 ?? 103 PFU mL-1) than among groups that survived exposure to VHSV (1.0-2.9 ?? 102 PFU mL-1); additionally, the proportion of cultures with no detectable virus was significantly greater (P = 0.0002) among fish that survived exposure to VHSV (39-47%) than among na??ve fish (3.3%). The optimized VREFT assay demonstrates promise for identifying VHSV exposure history and forecasting disease potential in populations of wild Pacific herring. ?? 2010 Blackwell Publishing Ltd.

  7. Effects of invasive European fire ants (Myrmica rubra on herring gull (Larus argentatus reproduction.

    Directory of Open Access Journals (Sweden)

    Luke E DeFisher

    Full Text Available Various invasive ant species have negatively affected reproductive success in birds by disrupting nest site selection, incubation patterns, food supply, and by direct predation on nestlings. Impacts can be particularly severe when non-native ants colonize seabird nesting islands where thousands of birds may nest in high densities on the ground or in burrows or crevices. Here we report on the first documented effects of Myrmica rubra, the European fire ant, on the reproduction of birds in its non-native range. We documented herring gulls (Larus argentatus on Appledore Island, Maine, engaging in more erratic incubation behaviors at nests infested by the ants. Newly-hatched chicks in some nests were swarmed by ants, leading to rapid chick death. Due to high overall rates of chick mortality, survival probabilities did not vary between nests with and without ant activity, however chick growth rates were slower at nests with ants than at ant-free nests. Ant infestation likely leads to longer-term fitness consequences because slower growth rates early in life may ultimately lead to lower post-fledging survival probabilities.

  8. Direct Effects of Microalgae and Protists on Herring (Clupea harengus Yolk Sac Larvae.

    Directory of Open Access Journals (Sweden)

    Björn Illing

    Full Text Available This study investigated effects of microalgae (Rhodomonas baltica and heterotrophic protists (Oxyrrhis marina on the daily growth, activity, condition and feeding success of Atlantic herring (Clupea harengus larvae from hatch, through the end of the endogenous (yolk sac period. Yolk sac larvae were reared in the presence and absence of microplankton and, each day, groups of larvae were provided access to copepods. Larvae reared with microalgae and protists exhibited precocious (2 days earlier and ≥ 60% increased feeding incidence on copepods compared to larvae reared in only seawater (SW. In the absence and presence of microalgae and protists, life span and growth trajectories of yolk sac larvae were similar and digestive enzyme activity (trypsin and nutritional condition (RNA-DNA ratio markedly declined in all larvae directly after yolk sac depletion. Thus, microplankton promoted early feeding but was not sufficient to alter life span and growth during the yolk sac phase. Given the importance of early feeding, field programs should place greater emphasis on the protozooplankton-ichthyoplankton link to better understand match-mismatch dynamics and bottom-up drivers of year class success in marine fish.

  9. Minors' capacity to refuse treatment: a reply to Gilmore and Herring.

    Science.gov (United States)

    Cave, Emma; Wallbank, Julie

    2012-01-01

    Re R and Re W allow a parent to consent to treatment a competent minor refuses, but the cases have not been tested post-Human Rights Act 1998. Gilmore and Herring offer a means by which they might be distinguished or sidelined. They interpret Gillick to say that in order to consent a minor need only have a full understanding of the particular treatment. They argue that the minors in Re R and Re W were refusing all treatment which requires a separate assessment of capacity-an assessment which was not made. We fear that this distinction would not be workable in clinical practice and argue that their interpretation of Gillick is flawed. From a clinician's point of view, competence cannot always be judged in relation to a specific treatment, but instead must relate to the decision. We show that a decision can incorporate more than one treatment, and more than one decision might be made about one treatment. A minor's understanding of a specific treatment is not always sufficient to demonstrate competence to make a decision. The result is that whilst there might be situations when a parent and a minor both have the power to consent to a particular treatment, they will not share concurrent powers in relation to the same decision. Consequently, a challenge to Re R and Re W, if forthcoming, would need to take a different form. We emphasise the necessity to minimise the dichotomy between legal consent and how consent works in medical practice.

  10. Direct Effects of Microalgae and Protists on Herring (Clupea harengus) Yolk Sac Larvae.

    Science.gov (United States)

    Illing, Björn; Moyano, Marta; Niemax, Jan; Peck, Myron A

    2015-01-01

    This study investigated effects of microalgae (Rhodomonas baltica) and heterotrophic protists (Oxyrrhis marina) on the daily growth, activity, condition and feeding success of Atlantic herring (Clupea harengus) larvae from hatch, through the end of the endogenous (yolk sac) period. Yolk sac larvae were reared in the presence and absence of microplankton and, each day, groups of larvae were provided access to copepods. Larvae reared with microalgae and protists exhibited precocious (2 days earlier) and ≥ 60% increased feeding incidence on copepods compared to larvae reared in only seawater (SW). In the absence and presence of microalgae and protists, life span and growth trajectories of yolk sac larvae were similar and digestive enzyme activity (trypsin) and nutritional condition (RNA-DNA ratio) markedly declined in all larvae directly after yolk sac depletion. Thus, microplankton promoted early feeding but was not sufficient to alter life span and growth during the yolk sac phase. Given the importance of early feeding, field programs should place greater emphasis on the protozooplankton-ichthyoplankton link to better understand match-mismatch dynamics and bottom-up drivers of year class success in marine fish.

  11. Preliminary analysis of the genetic variability of two natural beds of the Scallop Euvola ziczac (Linnaeus, 1758 in Brazil

    Directory of Open Access Journals (Sweden)

    Mercedes Gonzalez Wangüemert

    2000-01-01

    Full Text Available Euvola ziczac (formerly Pecten ziczac, a simultaneous hermaphroditic scallop was heavily fished in Brazil between 1972 and 1980. The production peaked in 1980 with 8,800 tons and was followed by the total collapse of the resource. In order to investigate the possible loss of genetic variability of the stock associated to overfishing and self-fertilization, the polymorphism of phosphoglucomutase (PGM and glucose phosphate isomerase (GPI was analyzed by electrophoresis of the adductor muscle of scallops from São Francisco (26° 20.583’S; 48° 16.507’W and Bom Abrigo (25° 28.735’S; 47° 37.621’W beds; the southern and northern extremes of the scallop fishing ground, respectively. Animals from São Francisco showed a strong deficiency of heterozygosity for GPI and PGM. In addition, PGM showed *exclusive alleles for each bed. Such results coupled with other information about the species suggested the following hypothesis: a the stock was a metapopulation with at least two populations; b some reproductive isolation might be occurring which might be influenced by conditions of larval transport and by the extremely low densities of scallops; c presently, the stock seemed to be mostly maintained through self-fertilization; d São Francisco could constitute a source-area, contributing with larvae and recruits to Bom Abrigo and other areas; e both beds were suffering a genetic homogenization more evident in São Francisco. Such hypothesis needed to be investigated in order to furnish guidelines for future programs of recovery and management of the resource.A vieira hermafrodita simultânea Euvola ziczac (anteriormente Pecten ziczac, foi intensamente explotada no Brasil entre os anos de 1972 e 1980. O máximo de produção foi atingido em 1980, com 8800 toneladas desembarcadas, após o qual ocorreu o total colapso do recurso. Com o objetivo de reconhecer uma possível perda de variabilidade genética do estoque associada à sobrepesca e

  12. Quality and shelf life of the gonad of lion's paw scallop transported and stored whole in refrigeration

    Directory of Open Access Journals (Sweden)

    Victor Manuel Ocaño-Higuera

    2015-05-01

    Full Text Available The effect of refrigerated 48h transport and 4 days storage on the quality and shelf life of the whole lion's paw scallop Nodipecten subnodosus gonad was evaluated. Proximal composition, adenosine 5´triphosphate (ATP and related products, K-value, total volatile bases (TVB-N, trimethylamine (TMA-N, pH, fatty acid profile and microbiological analyses were quantified. Gonad holds a significant composition of essential fatty acids while levels of gonadal ATP were initially low; moreover, K-value of the gonad remained constant. With respect to TVB-N and TMA-N, only the former exceeded allowed limits. The pH level showed no significant variation during storage and, despite the high level of TVB-N, according to the TMA-N as well as microbiological analyses it was demonstrated innocuity after 4 days under the transportation and storage conditions utilized.

  13. Genetic factors have a major effect on growth, number of vertebrae and otolith shape in Atlantic herring (Clupea harengus).

    Science.gov (United States)

    Berg, Florian; Almeland, Oda W; Skadal, Julie; Slotte, Aril; Andersson, Leif; Folkvord, Arild

    2018-01-01

    Atlantic herring, Clupea harengus, have complex population structures. Mixing of populations is known, but the extent of connectivity is still unclear. Phenotypic plasticity results in divergent phenotypes in response to environmental factors. A marked salinity gradient occurs from Atlantic Ocean (salinity 35) into the Baltic Sea (salinity range 2-12). Herring from both habitats display phenotypic and genetic variability. To explore how genetic factors and salinity influence phenotypic traits like growth, number of vertebrae and otolith shape an experimental population consisting of Atlantic purebreds and Atlantic/Baltic F1 hybrids were incubated and co-reared at two different salinities, 16 and 35, for three years. The F1-generation was repeatedly sampled to evaluate temporal variation. A von Bertalanffy growth model indicated that reared Atlantic purebreds had a higher maximum length (26.2 cm) than Atlantic/Baltic hybrids (24.8 cm) at salinity 35, but not at salinity 16 (25.0 and 24.8 cm, respectively). In contrast, Atlantic/Baltic hybrids achieved larger size-at-age than the wild caught Baltic parental group. Mean vertebral counts and otolith aspect ratios were higher for reared Atlantic purebreds than Atlantic/Baltic hybrids, consistent with the differences between parental groups. There were no significant differences in vertebral counts and otolith aspect ratios between herring with the same genotype but raised in different salinities. A Canonical Analysis of Principal Coordinates was applied to analyze the variation in wavelet coefficients that described otolith shape. The first discriminating axis identified the differences between Atlantic purebreds and Atlantic/Baltic hybrids, while the second axis represented salinity differences. Assigning otoliths based on genetic groups (Atlantic purebreds vs. Atlantic/Baltic hybrids) yielded higher classification success (~90%) than based on salinities (16 vs. 35; ~60%). Our results demonstrate that otolith shape and

  14. Genetic factors have a major effect on growth, number of vertebrae and otolith shape in Atlantic herring (Clupea harengus.

    Directory of Open Access Journals (Sweden)

    Florian Berg

    Full Text Available Atlantic herring, Clupea harengus, have complex population structures. Mixing of populations is known, but the extent of connectivity is still unclear. Phenotypic plasticity results in divergent phenotypes in response to environmental factors. A marked salinity gradient occurs from Atlantic Ocean (salinity 35 into the Baltic Sea (salinity range 2-12. Herring from both habitats display phenotypic and genetic variability. To explore how genetic factors and salinity influence phenotypic traits like growth, number of vertebrae and otolith shape an experimental population consisting of Atlantic purebreds and Atlantic/Baltic F1 hybrids were incubated and co-reared at two different salinities, 16 and 35, for three years. The F1-generation was repeatedly sampled to evaluate temporal variation. A von Bertalanffy growth model indicated that reared Atlantic purebreds had a higher maximum length (26.2 cm than Atlantic/Baltic hybrids (24.8 cm at salinity 35, but not at salinity 16 (25.0 and 24.8 cm, respectively. In contrast, Atlantic/Baltic hybrids achieved larger size-at-age than the wild caught Baltic parental group. Mean vertebral counts and otolith aspect ratios were higher for reared Atlantic purebreds than Atlantic/Baltic hybrids, consistent with the differences between parental groups. There were no significant differences in vertebral counts and otolith aspect ratios between herring with the same genotype but raised in different salinities. A Canonical Analysis of Principal Coordinates was applied to analyze the variation in wavelet coefficients that described otolith shape. The first discriminating axis identified the differences between Atlantic purebreds and Atlantic/Baltic hybrids, while the second axis represented salinity differences. Assigning otoliths based on genetic groups (Atlantic purebreds vs. Atlantic/Baltic hybrids yielded higher classification success (~90% than based on salinities (16 vs. 35; ~60%. Our results demonstrate that

  15. Evidence from the past: exploitation as cause of commercial extinction of autumn-spawning herring in the Gulf of Riga, Baltic Sea

    DEFF Research Database (Denmark)

    MacKenzie, Brian R; Ojaveer, Henn

    2018-01-01

    Historical marine ecology has shown that many exploited animal populations declined before their abundance was quantified by scientists. This situation applies for autumn-spawning herring (Clupea harengus) in the Baltic Sea. This stock used to be the dominant spawning group of herring in the early...... and biological data and conduct population development simulations to evaluate the hypothesis that exploitation may have been sufficient to lead the stock towards commercial extinction. We found that the estimated exploitation pattern, including exploitation of juveniles, was unsustainable and led to stock...

  16. Biomineralization changes with food supply confer juvenile scallops (Argopecten purpuratus) resistance to ocean acidification, supplement to: Ramajo, L; Marba, Núria; Prado, Luis; Peron, Sophie; Lardies, Marco A; Rodriguez-Navarro, Alejandro; Vargas, C A; Lagos, Nelson A; Duarte, Carlos M (2016): Biomineralization changes with food supply confer juvenile scallops (Argopecten purpuratus) resistance to ocean acidification. Global Change Biology, 22(6), 2025-2037

    KAUST Repository

    Ramajo, L; Marba, Nú ria; Prado, Luis; Peron, Sophie; Lardies, Marco A; Rodriguez-Navarro, Alejandro; Vargas, C A; Lagos, Nelson A; Duarte, Carlos M.

    2016-01-01

    to control (pH 8.0) and low pH (pH 7.6) conditions using three food supply treatments (high, intermediate, and low). We found that pH and food levels had additive effects on the physiological response of the juvenile scallops. Metabolic rates, shell growth

  17. A phenomenological study of ripening of salted herring. Assessing homogeneity of data from different countries and laboratories

    DEFF Research Database (Denmark)

    Bro, R.; Nielsen, Henrik Hauch; Stefánsson, G.

    2002-01-01

    of treatments and analyzed in different laboratories. The question considered here is whether these data can be assumed to be one homogeneous set of data pertaining to ripening of salted herring or whether data from different labs, stocks, etc. must be considered independently. This is of importance for further...... easily be envisioned, it was exactly the need for a more intuitive and visual test that prompted this work, developing different exploration tools that visually make it clear how well the data can be assumed to derive from the same population. Subsequently analyzing the data as one homogeneous group...

  18. Exploring the multidimensional nature of stock structure: a case study on herring dynamics in a transition area

    DEFF Research Database (Denmark)

    Worsøe Clausen, Lotte

    occupy areas without much environmental structuring and extensive mixing between populations occur. Many species of marine fishes have the capacity of dispersing over vast geographical areas, either passively by drifting eggs and larvae following ocean currents, or actively by migration of juveniles...... will aid a sustainable aggregated management of a fishery on a mixed herring stock. It will facilitate protecting the weaker populations from over harvesting in a mixed fishery and thus maintain the diversity and in turn the resilience of the stock to a fishery...

  19. The fate of eggs and larvae of three pelagic species, mackerel (Scomber scombrus, horse mackerel (Trachurus trachurus and sardine (Sardina pilchardus in relation to prevailing currents in the Bay of Biscay: Could they affect larval survival?

    Directory of Open Access Journals (Sweden)

    Paula Alvarez

    2012-05-01

    Full Text Available The spatial distribution of eggs and larvae of three pelagic species, mackerel, horse mackerel and sardine, in the Bay of Biscay was studied in 1998, 2001 and 2004. The spatial distribution was clearly different between the years studied and corresponds quite precisely to different water circulation regimes. Mackerel and horse mackerel larvae are more affected by the prevailing currents than sardine, as their spawning grounds are located offshore, far from the shelf break where the current velocities are higher. Survival rates for mackerel and horse mackerel were higher in 2001, when the offshore larval transport was stronger. However, for sardine, the mortality rate hardly varied between years. The abundance of 25-day-old larvae, considered as an index of the survival rate, appears to be a good recruitment indicator, at least for 1998, 2001 and 2004. Our results did not support the hypothesis of Bakun (1996, which states that dispersion of early life stages towards open ocean waters should cause high larval mortality. At least under the conditions observed for the years studied, the retention of larvae offshore appears to have a positive effect on larval growth and/or survival.

  20. Identification and Characterization of Main Allergic Proteins in Cooked Wolf Herring Fish.

    Science.gov (United States)

    Mohamadi, Mohsen; Falak, Reza; Mokhtarian, Kobra; Khoramizadeh, Mohammad Reza; Sadroddiny, Esmaeil; Kardar, Gholam Ali

    2016-10-01

    Our aim in this study was to identify and characterize allergic proteins in cooked wolf herring fish. We heated the crude extract alternatively at 50, 60, 70, 80, 90, and 100°C for one hour and results were compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Also, proteins were immunoblotted with fish-sensitive patients' sera. The major allergenic proteins were identified via mass spectrometry. These allergenic proteins were then purified by anion exchange chromatography and the IgE-immunoreactivity of the fractions was compared with the crude extracts via disk enzyme-linked immunosorbent assay (ELISA). SDS-PAGE of the crude extract showed more than 15 distinct protein bands. Five of these proteins, with apparent molecular weights of 12, 18, 24, 38, and 51 kDa, were only observed in the 100°C heated extract. Immunoblotting of the heated extract revealed that the 12 and 51 kDa proteins were IgE-immunoreactive with 88 percent of fish-sensitive patient sera while the 24 and 38 kDa proteins reacted with 33.3 and 55.5 percent of fish-sensitive patient sera, respectively. Mass spectrometry of the 12, 38, and 51 kDa proteins revealed that all three were parvalbumin oligomers. Disk ELISA results showed that 20 of 25 and 14 of 25 fish-allergic patients' sera were IgE-reactive with purified oligomeric parvalbumin-coated and crude extract-coated disks, respectively. Parvalbumin and its oligomers are the main allergenic molecules in cooked fish. Therefore, an enriched or purified fraction containing this protein could be a useful source of allergen for applications in ELISA-based immunoassays and could discriminate fish-allergic patients who can tolerate cooked fish from those who cannot.

  1. Stock enhancement or sea ranching? Insights from monitoring the genetic diversity, relatedness and effective population size in a seeded great scallop population (Pecten maximus)

    OpenAIRE

    Morvezen, R; Boudry, P; Laroche, J; Charrier, G

    2016-01-01

    The mass release of hatchery-propagated stocks raises numerous questions concerning its efficiency in terms of local recruitment and effect on the genetic diversity of wild populations. A seeding program, consisting of mass release of hatchery-produced juveniles in the local naturally occurring population of great scallops (Pecten maximus L.), was initiated in the early 1980s in the Bay of Brest (France). The present study aims at evaluating whether this seeding program leads to actual popula...

  2. Risks and benefits of compost-like materials prepared by the thermal treatment of raw scallop hepatopancreas for supplying cadmium and the growth of alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Ushijima, Kensuke; Fukushima, Masami; Kanno, Shinya; Kanno, Itoko; Ohnishi, Mitsuhiro

    2016-01-01

    Scallop hepatopancreas, fishery waste, contains relatively high levels of Cd and organic nitrogen compounds, the latter of which represent a fertilizer. In this study, raw scallop hepatopancreas tissue was thermally treated with sawdust and red loam in the presence of an iron catalyst to produce compost-like materials (CLMs). Two CLM samples were prepared by varying the content of raw scallop hepatopancreas tissue: 46 wt.% for CLM-1 and 18 wt.% for CLM-2. Mixtures of control soil (CTL) and CLMs (CLM content: 10 and 25 wt.%) were examined for the growth of alfalfa (Medicago sativa L.) to evaluate the risks and benefits of using this material for fertilization. The Cd content in shoots and roots of alfalfa, that were grown in the presence of CLMs, was significantly higher than those for the plants grown in the CTL, indicating that Cd had accumulated in the plants from CLMs. The accumulation of Cd in the alfalfa roots was quite high in the case of the 25% CLM-1 sample. However, alfalfa growth was significantly promoted in the presence of 10% CLM-1. This can be attributed to the higher levels of nitrogen and humic substances, which serve as fertilizer components. Although the fertilization effect in case of CLM-1showed a potential benefit, the accumulation of Cd in alfalfa was clearly increased in the presence of both CLMs. In conclusion, the use of CLMs produced from raw scallop hepatopancreas tissue can be considered to have a desirable benefit from standpoint of its use as fertilizer, but is accompanied by a risk of the accumulation of Cd in alfalfa plants.

  3. Determination of trace elements in scallop and fish otolith by instrumental neutron activation analysis using anti-coincidence and coincidence counting methods

    International Nuclear Information System (INIS)

    Suzuki, Shogo; Okada, Yukiko; Hirai, Shoji

    2005-01-01

    Trace element concentrations in scallop reference material and fish otolith certified reference materials prepared at the National Institute for Environmental Studies (NIES) of Japan were determined by instrumental neutron activation analysis (INAA). Nine aliquots of scallop sample (ca. 252∼507 mg) and five aliquots of fish otolith sample (ca. 502 ∼ 988 mg) and comparative standards were irradiated for a short time (10 s) at a thermal neutron flux of 1.5 x 10 12 n cm -2 s -1 (pneumatic transfer) and for a long time (6 h) at a thermal neutron flux of 3.7 x 10 12 n cm -2 s -1 (central thimble) in the Rikkyo University Research Reactor (100 kW). The irradiated samples were measured by conventional γ-ray spectrometry using a coaxial Ge detector, and by anti-coincidence and coincidence γ-ray spectrometry with a coaxial Ge detector and a well-type NaI (Tl) detector to determine as many trace elements as possible with high sensitivity. The concentrations of 34 elements of the NIES No.15 scallop reference material and 16 elements of the NIES No.22 fish otolith CRM were determined. Using the coincidence counting method to determine Se, Ba and Hf, the lower limit of the determination was improved by 2 times compared with the conventional counting method. (author)

  4. Effects of the toxic dinoflagellate, Gymnodinium catenatum on hydrolytic and antioxidant enzymes, in tissues of the giant lions-paw scallop Nodipecten subnodosus.

    Science.gov (United States)

    Estrada, Norma; de Jesús Romero, Maria; Campa-Córdova, Angel; Luna, Antonio; Ascencio, Felipe

    2007-11-01

    This study documents effects of the toxic dinoflagellate Gymnodinium catenatum, a producer of paralytic shellfish poison, on juvenile farmed (5.9+/-0.39 cm) giant lions-paw scallop Nodipecten subnodosus. Scallops were fed bloom concentrations of toxic dinoflagellate G. catenatum for 7 h. The effect of the toxic dinoflagellate in different tissues was determined by analysis of antioxidant enzymes (catalase, superoxide dismutase, gluthathione peroxidase), thiobarbituric acid reactive substances (lipid peroxidation), and hydrolytic enzymes (proteases, glycosidases, phosphatases, lipases, and esterases). Histopathological photos record the effects of the toxic dinoflagellate in various tissues. The results show that juvenile lions-paw scallops produce pseudo-feces, partially close their shell, increase melanization, and aggregate hemocytes. Several enzymes were affected and could serve as biological markers. In general, the adductor muscle was not affected. In the digestive gland, some enzymes could be the result of defensive and digestive processes. Gills and mantle tissue were markedly affected because these sites respond first to toxic dinoflagellates, leading to the idea that proteolytic cascades could be involved.

  5. Experimental study on the effective utilization of combusted scallop cultivation waste. Hotate yoshoku haikibutsu no yuko riyo no tame no shokyaku shori joken no jikkenteki kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Hase, Y [The Tohoku Electric Power Co. Inc., Sendai (Japan)

    1993-06-15

    Discussions are given on treatment methods that can utilize scallop cultivation wastes effectively. Tests to incinerate and convert the main scallop shell content, calcium carbonate, into calcium oxide (CaO) discovered optimal incinerating conditions of a temperature at 750[degree]C and a duration for 30 minutes, and 700[degree]C and one hour. CaO content in burnt shells was so high that they could be used as a raw material for quick lime. In the incineration and conversion tests on wastes from shell processing factories, the result of X-ray diffraction in chemically combined conditions of inorganics before and after the incineration revealed that the main component Ca existed as CaCO3, which may be converted into CaO when incinerated. The result also showed that the second component, Mg, existed as MgO after the incineration. From the above results, incinerating the wastes from shell processing factories at 750[degree]C would be capable of incinerating organic components completely and reducing their weights. Because the inorganic residues have high CaO content, they could be utilized effectively as a quick lime raw material. Although shells to be incinerated have low salt content because of having been boiled, salt content in scallop processing factory wastes will require discussions on possibilities of its corroding an incineration furnace. 3 figs., 7 tabs.

  6. Organ distribution and food safety aspects of cadmium and lead in great scallops, Pecten maximus L., and Horse Mussels, Modiolus modiolus L., from Norwegian waters.

    Science.gov (United States)

    Julshamn, Kaare; Duinker, Arne; Frantzen, Sylvia; Torkildsen, Lise; Maage, Amund

    2008-04-01

    The purpose of the study was to determine the levels and organ distribution of the potentially harmful inorganic elements cadmium and lead in great scallops and horse mussels from unpolluted Norwegian waters. The scallops far exceeded the EU-limit for cadmium in bivalves when all soft tissues were analysed. When only muscle and gonad were included, however, the level of cadmium was acceptable, because cadmium accumulated in the digestive gland with a mean of 52 mg/kg ww (wet weight). In horse mussel, lead was the most problematic element and the concentration varied from 1.4 to 6.6 mg/kg ww with a mean of 3.7 mg/kg ww, exceeding the EU limit of 1.5 mg Pb/kg. The highest concentration of lead was found in the kidney with an average of 120 mg/kg ww and with a maximum value of 240 mg/kg ww. The kidney tissue accounted for approximately 94% of the lead burden in the horse mussel. In order to consume these bivalves, only muscle and gonad of great scallops should be used for consumption and the kidney of horse mussel should be removed prior to consumption.

  7. Identifying molecular effects of diet through systems biology: influence of herring diet on sterol metabolism and protein turnover in mice.

    Directory of Open Access Journals (Sweden)

    Intawat Nookaew

    Full Text Available BACKGROUND: Changes in lifestyle have resulted in an epidemic development of obesity-related diseases that challenge the healthcare systems worldwide. To develop strategies to tackle this problem the focus is on diet to prevent the development of obesity-associated diseases such as cardiovascular disease (CVD. This will require methods for linking nutrient intake with specific metabolic processes in different tissues. METHODOLOGY/PRINCIPAL FINDING: Low-density lipoprotein receptor-deficient (Ldlr -/- mice were fed a high fat/high sugar diet to mimic a westernized diet, being a major reason for development of obesity and atherosclerosis. The diets were supplemented with either beef or herring, and matched in macronutrient contents. Body composition, plasma lipids and aortic lesion areas were measured. Transcriptomes of metabolically important tissues, e.g. liver, muscle and adipose tissue were analyzed by an integrated approach with metabolic networks to directly map the metabolic effects of diet in these different tissues. Our analysis revealed a reduction in sterol metabolism and protein turnover at the transcriptional level in herring-fed mice. CONCLUSION: This study shows that an integrated analysis of transcriptome data using metabolic networks resulted in the identification of signature pathways. This could not have been achieved using standard clustering methods. In particular, this systems biology analysis could enrich the information content of biomedical or nutritional data where subtle changes in several tissues together affects body metabolism or disease progression. This could be applied to improve diets for subjects exposed to health risks associated with obesity.

  8. GPS tracking data of Lesser Black-backed Gulls and Herring Gulls breeding at the southern North Sea coast.

    Science.gov (United States)

    Stienen, Eric W M; Desmet, Peter; Aelterman, Bart; Courtens, Wouter; Feys, Simon; Vanermen, Nicolas; Verstraete, Hilbran; de Walle, Marc Van; Deneudt, Klaas; Hernandez, Francisco; Houthoofdt, Robin; Vanhoorne, Bart; Bouten, Willem; Buijs, Roland-Jan; Kavelaars, Marwa M; Müller, Wendt; Herman, David; Matheve, Hans; Sotillo, Alejandro; Lens, Luc

    2016-01-01

    In this data paper, Bird tracking - GPS tracking of Lesser Black-backed Gulls and Herring Gulls breeding at the southern North Sea coast is described, a species occurrence dataset published by the Research Institute for Nature and Forest (INBO). The dataset (version 5.5) contains close to 2.5 million occurrences, recorded by 101 GPS trackers mounted on 75 Lesser Black-backed Gulls and 26 Herring Gulls breeding at the Belgian and Dutch coast. The trackers were developed by the University of Amsterdam Bird Tracking System (UvA-BiTS, http://www.uva-bits.nl). These automatically record and transmit bird movements, which allows us and others to study their habitat use and migration behaviour in great detail. Our bird tracking network is operational since 2013. It is funded for LifeWatch by the Hercules Foundation and maintained in collaboration with UvA-BiTS and the Flanders Marine Institute (VLIZ). The recorded data are periodically released in bulk as open data (http://dataset.inbo.be/bird-tracking-gull-occurrences), and are also accessible through CartoDB and the Global Biodiversity Information Facility (GBIF).

  9. A consensus microsatellite-based linkage map for the hermaphroditic bay scallop (Argopecten irradians and its application in size-related QTL analysis.

    Directory of Open Access Journals (Sweden)

    Hongjun Li

    Full Text Available Bay scallop (Argopecten irradians is one of the most economically important aquaculture species in China. In this study, we constructed a consensus microsatellite-based genetic linkage map with a mapping panel containing two hybrid backcross-like families involving two subspecies of bay scallop, A. i. irradians and A. i. concentricus. One hundred sixty-one microsatellite and one phenotypic (shell color markers were mapped to 16 linkage groups (LGs, which corresponds to the haploid chromosome number of bay scallop. The sex-specific map was 779.2 cM and 781.6 cM long in female and male, respectively, whereas the sex-averaged map spanned 849.3 cM. The average resolution of integrated map was 5.9 cM/locus and the estimated coverage was 81.3%. The proportion of distorted markers occurred more in the hybrid parents, suggesting that the segregation distortion was possibly resulted from heterospecific interaction between genomes of two subspecies of bay scallop. The overall female-to-male recombination rate was 1.13:1 across all linked markers in common to both parents, and considerable differences in recombination also existed among different parents in both families. Four size-related traits, including shell length (SL, shell height (SH, shell width (SW and total weight (TW were measured for quantitative trait loci (QTL analysis. Three significant and six suggestive QTL were detected on five LGs. Among the three significant QTL, two (qSW-10 and qTW-10, controlling SW and TW, respectively were mapped on the same region near marker AiAD121 on LG10 and explained 20.5% and 27.7% of the phenotypic variance, while the third (qSH-7, controlling SH was located on LG7 and accounted for 15.8% of the phenotypic variance. Six suggestive QTL were detected on four different LGs. The linkage map and size-related QTL obtained in this study may facilitate marker-assisted selection (MAS in bay scallop.

  10. Histopathology and cytogenetic evaluation of Pacific herring larvae exposed to petroleum hydrocarbons in the laboratory or in Prince William Sound, Alaska, after the Exxon Valdez oil spill

    International Nuclear Information System (INIS)

    Marty, G.D.; Hinton, D.E.; Brown, E.D.

    1997-01-01

    Following the Exxon Valdez oil spill in Prince William Sound, Alaska, Pacific herring larvae samples from oiled sites showed ascites, pericardial edema, and genotoxic damage. Laboratory study confirmed that these lesions were consistent with oil exposure. In a laboratory experiment, Pacific herring eggs were exposed to an oil-water dispersion of Prudhoe Bay crude oil and sampled for histopathology less than 24 h after hatching. Effects were significant at the 0.48 mg/L dose. Lesions included ascites, heptocellular vacuolar change and degeneration or necrosis of skeletal myocytes, retinal cells, and developing brain cells. Lesions in field-sampled larvae were consistent with higher mortality rates documented in larvae from oiled sites. In both field and laboratory experiments, ascites was the most significant lesion related to oil exposure. Decreased growth in larvae from oiled sites was also consistent with findings in three other laboratory studies with Pacific herring. This study concluded that if a large proportion of a population is exposed to contamination during early life stages, impacts on subsequent recruitment may be significant. However, estimates of the proportion of Pacific herring year-class affected by the Exxon Valdez oil spill vary from 4 per cent to 50 per cent. Since recruitment of the 1989 year-class was also poor in Sitka Sound (the control site), it was suggested that oceanic variables might have been more significant in limiting recruitment of the 1989 year-class in Prince William Sound than was the oil spill. 40 refs., 3 tabs., 7 figs

  11. Comparative biology and population mixing among local, coastal and offshore Atlantic herring (Clupea harengus) in the North Sea, Skagerrak, Kattegat and western Baltic

    Science.gov (United States)

    Slotte, Aril; Johannessen, Arne; Kvamme, Cecilie; Clausen, Lotte Worsøe; Nash, Richard D. M.

    2017-01-01

    The population structure of Atlantic herring (Clupea harengus) from 13 local, coastal and offshore areas of the North Sea, Skagerrak, Kattegat and western Baltic (northeast Atlantic) was studied using biological and environmental data from 1970–2015. The objective was to identify distinct populations by comparing variability in the temporal and spatial phenotypic characteristics and evaluate the potential for mixing of populations in time and space. The populations varied in biological characteristics such as mean vertebral counts (VS), growth and maturity ogives. Generalized additive models indicated temporally stable VS in the North Sea and western Baltic, whereas intra-annual temporal variation of VS occurred in other areas. High variability of VS within a population was not affected by environmental factors such as temperature and salinity. Consequently, seasonal VS variability can be explained by the presence or absence of herring populations as they migrate between areas. The three main populations identified in this paper correspond to the three managed stocks in this area: Norwegian spring spawners (NSS), western Baltic spring spawners (WBSS) and North Sea autumn spawners (NSAS). In addition, several local populations were identified in fjords or lakes along the coast, but our analyses could not detect direct mixing of local populations with the three main populations. Our results highlight the importance of recognizing herring dynamics and understanding the mixing of populations as a challenge for management of herring. PMID:29084258

  12. Environmental conditions, early life stages distributions and larval feeding of patagonian sprat Sprattusfuegensis and common sardine Strangomerabentincki in fjords and channels of the northern Chilean patagonia

    Science.gov (United States)

    Contreras, Tabit; Castro, Leonardo R.; Montecinos, Sandra; Gonzalez, Humberto E.; Soto, Samuel; Muñoz, Maria I.; Palma, Sergio

    2014-12-01

    We assessed ontogenetic changes in distribution and feeding of the Patagonian sprat Sprattus fuegensis and common sardine Strangomera bentincki, and their association with environmental characteristics (hydrography, larval food, gelatinous zooplankton predators), and actual feeding from inshore to offshore areas of the Chilean Patagonia. During the springs of 2007 and 2008, S. bentincki egg and larvae were present north of the Taitao Peninsula (47°S) and S. fuegensis was found to the south of the peninsula. Along the inshore-offshore axis, distributions also differed: while eggs and early larval stages of S. bentincki occurred inshore and seawards, larger larvae occurred mostly seawards. The opposite was observed in S. fuegensis. However, distributions of both species followed the same rule, determined by the size of their prey: eggs and early larval stages occurred in areas of higher abundance of small prey sizes, and larger larvae coincided with the highest abundances of larger prey sizes. No relationship was detected between potential gelatinous predators and the egg and larval distributions of both fish species. Mean ingested prey sizes in both species increased as larvae grew, while maintaining the capacity to feed on small sized items. This ontogenetic feeding pattern and the distributions linked to prey seem to be beneficial in order to take advantage of short term food pulses and to overcome the strong changes in environmental conditions east to west from fjords to open waters.

  13. The commercial fishing of sardines (Triportheus spp. landed in the fishery market of Porto Velho, Rondônia (1990-2004: Yield and general profile

    Directory of Open Access Journals (Sweden)

    Luiz Jardim de Queiroz

    2008-09-01

    Full Text Available The profile of sardine fishing, the Triportheus species, landed at the fishing market of Porto Velho (RO between 1990 and 2004, was established by analyzing variations in the intra- and inter-annual yield as well as the length and general characteristics of the fishing. The annual production varied from 15,372 to 78,340kg (average = 33,482kg, totaling 5.46% of the overall production during the studied period. These variations can be related to the changes in the fishermen’s effort, as a reflex to searching for alternatives for fishing, or related to the level of the river, since the greatest annual yields were preceded by years of having more intense flooding. The monthly production varied from 0 to 21,456kg (average=2,876kg. Greater values of production were obtained in periods of lower hydrological levels (dry season [r(Pearson= -0.53. p<0.0001]. The average capture per fisherman/day between 2003 and 2004 was 9.27kg. Rivers were the main environment from which the captures took place, mainly from the Jamari, Machado and Novo Aripuanã rivers, that are tributaries of the Madeira River. The lengths of the fishes varied from 70 to 380mm and presented a bimodal pattern, suggesting the presence of more than one species within the captures.

  14. Trace element distribution during the reproductive cycle of female and male spiny and Pacific scallops, with implications for biomonitoring

    International Nuclear Information System (INIS)

    Norum, Ulrik; Lai, Vivian W.-M.; Cullen, William R.

    2005-01-01

    Trace element concentrations and contents in gills, gonad, kidneys, mantle, muscle and remainder during the reproductive cycle of female and male spiny and Pacific scallops, from the Strait of Georgia, BC, Canada, were quantified by using ICPMS. The elements investigated were chromium, manganese, iron, cobalt, nickel, selenium, molybdenum, cadmium, tin and mercury. For all ten elements, the tissue distribution was to some extent influenced by species, sex and reproductive status. The implications of the present study in relation to the design of biomonitoring programmes are: (1) care should be taken to ensure an equal/constant sex composition when making interannual comparisons of pooled samples. Preferably the sexes should be monitored separately. (2) the practice of obtaining pooled samples in the interspawn phase is applicable only to monitoring long-term trends in contaminant levels, while the reproductive status should be heeded when studying short-term changes. (3) the present study confirms that direct temporal or spatial comparisons of absolute accumulated element concentrations are only valid intraspecifically

  15. Comparative analysis of the reproductive strategy of lion's paw scallop Nodipecten subnodosus in Baja California Sur, Mexico

    Directory of Open Access Journals (Sweden)

    Marco A Angel-Dapa

    2015-07-01

    Full Text Available The reproductive strategy of lion's paw scallop Nodipecten subnodosus was evaluated in a culturing system in Bahía Tortugas, Baja California Sur, Mexico during an annual cycle, comparing its response with data previously reported at other localities. High frequencies of ripe gonads throughout the year indicate that reproduction was continuous, with two main ripening/spawning events: July-September and December-March. A continuous breeding is also reported for the species in Bahía Magdalena, Bahía Juncalito, and Bahía de Los Angeles. These eutrophic areas are Biological Active Centers where gametogenesis appears to be regulated by the energy taken from recently ingested food following an opportunistic strategy. However, the digestive gland index decreased and the muscle indices increased during one of the breeding peaks, suggesting that some stored reserves are also used to sustain gametogenesis (conservative strategy partially. High incidences of atretic oocytes are likely associated with atypical daily variations in water temperature from May through September (12 to 33°C, or with stressful conditions in the culturing system in summer. Despite this, the culturing system set in Bahía Tortugas appears beneficial for a continuous reproduction of N. subnodosus.

  16. Alterations of valve closing behavior in juvenile Catarina scallops (Argopecten ventricosus Sowerby, 1842) exposed to toxic metals.

    Science.gov (United States)

    Sobrino-Figueroa, A; Cáceres-Martínez, C

    2009-11-01

    We conducted an evaluation of alterations produced in the valve closing speed of juvenile Argopecten ventricosus (Catarina scallop) exposed to the metals cadmium, chromium and lead, because of the connection of this response to the state of health of the mollusk. Bioassays were conducted with 50 juveniles (length 3 +/- 0.5 cm) exposed to 0.02, 0.1, 0.2 mg Cd l(-1); 0.1, 0.5, 1.0 mg Cr l(-1); 0.04, 0.2, 0.4 mg Pb l(-1) and 0.8 and 1.6 mg Cd + Cr + Pb l(-1) for 480 h. The average valve closing speed at the end of the experiment was under 1 s in the control group, from 2 to 3.6 s in the bioassays with cadmium, from 1.4 to 3.4 s with chromium, from 3 to 12 s with lead, and from 12 to 15 s with the metal mixtures. It was found that there are significant differences between the values recorded in assays with metals and the control (P < 0.05). The retardation of valve closing in the organisms exposed to toxic substances is probably caused by damage to the sensory cilia located on the edge of the mantle.

  17. Production of L-lactic Acid from Biomass Wastes Using Scallop Crude Enzymes and Novel Lactic Acid Bacterium

    Science.gov (United States)

    Yanagisawa, Mitsunori; Nakamura, Kanami; Nakasaki, Kiyohiko

    In the present study, biomass waste raw materials including paper mill sludge, bamboo, sea lettuce, and shochu residue (from a distiller) and crude enzymes derived from inedible and discarded scallop parts were used to produce L-lactic acid for the raw material of biodegradable plastic poly-lactic acid. The activities of cellulase and amylase in the crude enzymes were 22 and 170units/L, respectively, and L-lactic acid was produced from every of the above mentioned biomass wastes, by the method of liquid-state simultaneous saccharification and fermentation (SSF) . The L-lactic acid concentrations produced from sea lettuce and shochu residue, which contain high concentration of starch were 3.6 and 9.3g/L, respectively, and corresponded to greater than 25% of the conversion of glucans contained in these biomass wastes. Furthermore, using the solid state SSF method, concentrations as high as 13g/L of L-lactic acid were obtained from sea lettuce and 26g/L were obtained from shochu residue.

  18. High-power free-electron laser amplifier using a scalloped electron beam and a two-stage wiggler

    Directory of Open Access Journals (Sweden)

    D. C. Nguyen

    2006-05-01

    Full Text Available High-power free-electron laser (FEL amplifiers present many practical design and construction problems. One such problem is possible damage to any optical beam control elements beyond the wiggler. The ability to increase the optical beam’s divergence angle after the wiggler, thereby reducing the intensity on the first optical element, is important to minimize such damage. One proposal to accomplish this optical beam spreading is to pinch the electron beam thereby focusing the radiation as well. In this paper, we analyze an approach that relies on the natural betatron motion to pinch the electron beam near the end of the wiggler. We also consider a step-tapered, two-stage wiggler to enhance the efficiency. The combination of a pinched electron beam and step-taper wiggler leads to additional optical guiding of the optical beam. This novel configuration is studied in simulation using the MEDUSA code. For a representative set of beam and wiggler parameters, we discuss (i the effect of the scalloped beam on the interaction in the FEL and on the focusing and propagation of the radiation, and (ii the efficiency enhancement in the two-stage wiggler.

  19. Selection by higher-order effects of salinity and bacteria on early life-stages of Western Baltic spring-spawning herring.

    Science.gov (United States)

    Poirier, Maude; Listmann, Luisa; Roth, Olivia

    2017-07-01

    Habitat stratification by abiotic and biotic factors initiates divergence of populations and leads to ecological speciation. In contrast to fully marine waters, the Baltic Sea is stratified by a salinity gradient that strongly affects fish physiology, distribution, diversity and virulence of important marine pathogens. Animals thus face the challenge to simultaneously adapt to the concurrent salinity and cope with the selection imposed by the changing pathogenic virulence. Western Baltic spring-spawning herring ( Clupea harengus ) migrate to spawning grounds characterized by different salinities to which herring are supposedly adapted. We hypothesized that herring populations do not only have to cope with different salinity levels but that they are simultaneously exposed to higher-order effects that accompany the shifts in salinity, that is induced pathogenicity of Vibrio bacteria in lower saline waters. To experimentally evaluate this, adults of two populations were caught in their spawning grounds and fully reciprocally crossed within and between populations. Larvae were reared at three salinity levels, representing the spawning ground salinity of each of the two populations, or Atlantic salinity conditions resembling the phylogenetic origin of Clupea harengus . In addition, larvae were exposed to a Vibrio spp . infection. Life-history traits and gene expression analysis served as response variables. Herring seem adapted to Baltic Sea conditions and cope better with low saline waters. However, upon a bacterial infection, herring larvae suffer more when kept at lower salinities implying reduced resistance against Vibrio or higher Vibrio virulence. In the context of recent climate change with less saline marine waters in the Baltic Sea, such interactions may constitute key future stressors.

  20. Contaminant levels in Herring (Larus argentatus) and Great Black-backed Gull (Larus marinus) eggs from colonies in the New York harbor complex between 2012 and 2013.

    Science.gov (United States)

    Burger, Joanna; Elbin, Susan

    2015-03-01

    Birds living in coastal areas are exposed to severe storms and tidal flooding during the nesting season, but also to contaminants that move up the food chain from the water column and sediment to their prey items. We examine metals in Herring Gull (Larus argentatus) and Great Black-backed Gull (Larus marinus) eggs collected from the New York/New Jersey harbor estuary in 2012 and in 2013 to determine if there were significant yearly differences in metal levels. We test the null hypothesis that there were no significant yearly differences in metal levels. We investigate whether there were consistent differences in metals from 2012 to 2013 that might suggest a storm-related effect because Superstorm Sandy landed in New Jersey in October 2012 with high winds and extensive flooding, and view this research as exploratory. Except for arsenic, there were significant inter-year variations in the mean levels for all colonies combined for Herring Gull, and for lead, mercury and selenium for Great Black-backed Gulls. All metal levels in 2013 were less than in 2012, except for lead. These differences were present for individual colonies as well. Metal levels varied significantly among islands for Herring Gulls in both years (except for cadmium in 2013). No one colony had the highest levels of all metals for Herring Gulls. A long term data set on mercury levels in Herring Gulls indicated that the differences between 2012 and 2013 were greater than usual. Several different factors could account for these differences, and these are discussed.

  1. Temporal trends in dioxins (polychlorinated dibenzo-p-dioxin and dibenzofurans) and dioxin-like polychlorinated biphenyls in Baltic herring (Clupea harengus).

    Science.gov (United States)

    Miller, Aroha; Hedman, Jenny E; Nyberg, Elisabeth; Haglund, Peter; Cousins, Ian T; Wiberg, Karin; Bignert, Anders

    2013-08-15

    Polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyl (dl-PCBs) concentrations in Baltic herring (Clupea harengus) have been relatively stable since the mid to late 1990s. It is unclear why concentrations in Baltic herring are not following the observed decreases in other environmental matrices. Here, changes in long-term temporal trends in Baltic herring were examined. A number of biological variables were examined alongside the temporal trends to investigate whether fish biology e.g., growth (length, weight, age), lipid content, reproductive phase or fishing date may provide an explanation for the temporal trends observed. Significant (p<0.05) decreasing trends were observed for PCDD/F toxic equivalents (TEQPCDD/F) at three of the four sites (lipid weight (lw) and wet weight (ww), Swedish west coast lw only); however, other TEQ values e.g., TEQPCDD, TEQPCDF, TEQdl-PCB, TEQPCDD/F+dl-PCB were inconsistent, decreasing at some sites but not others. In the most recent 10 years of data, fewer significant decreases were seen overall. Over the examined time period, significant decreases (Bothnian Bay, p<0.01, southern Baltic Proper, p<0.02) and increases (Swedish west coast, p<0.02) in lipid content, growth dilution or lack thereof, and significant changes in age were observed. However herring were not randomly selected which biases this result. Continual efforts to decrease PCDD/F and dl-PCB emissions and to locate/reduce hotspots are necessary, while bearing in mind that herring biology may be impeding faster decreases of these chemicals. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Evaluating signals of oil spill impacts, climate, and species interactions in Pacific herring and Pacific salmon populations in Prince William Sound and Copper River, Alaska.

    Directory of Open Access Journals (Sweden)

    Eric J Ward

    Full Text Available The Exxon Valdez oil spill occurred in March 1989 in Prince William Sound, Alaska, and was one of the worst environmental disasters on record in the United States. Despite long-term data collection over the nearly three decades since the spill, tremendous uncertainty remains as to how significantly the spill affected fishery resources. Pacific herring (Clupea pallasii and some wild Pacific salmon populations (Oncorhynchus spp. in Prince William Sound declined in the early 1990s, and have not returned to the population sizes observed in the 1980s. Discerning if, or how much of, this decline resulted from the oil spill has been difficult because a number of other physical and ecological drivers are confounded temporally with the spill; some of these drivers include environmental variability or changing climate regimes, increased production of hatchery salmon in the region, and increases in populations of potential predators. Using data pre- and post-spill, we applied time-series methods to evaluate support for whether and how herring and salmon productivity has been affected by each of five drivers: (1 density dependence, (2 the EVOS event, (3 changing environmental conditions, (4 interspecific competition on juvenile fish, and (5 predation and competition from adult fish or, in the case of herring, humpback whales. Our results showed support for intraspecific density-dependent effects in herring, sockeye, and Chinook salmon, with little overall support for an oil spill effect. Of the salmon species, the largest driver was the negative impact of adult pink salmon returns on sockeye salmon productivity. Herring productivity was most strongly affected by changing environmental conditions; specifically, freshwater discharge into the Gulf of Alaska was linked to a series of recruitment failures-before, during, and after EVOS. These results highlight the need to better understand long terms impacts of pink salmon on food webs, as well as the

  3. Egg-larval mortality of Pacific herring in Prince William Sound, Alaska, after the Exxon Valdez oil spill

    International Nuclear Information System (INIS)

    McGurk, M. D.; Brown, E. D.

    1996-01-01

    Experiments were carried out to test the hypothesis that instantaneous daily rates of egg-larval mortality of Pacific herring were higher at two oil-exposed sites than at two other sites not so exposed. Results showed that egg-larval mortality was twice as great in the oil-exposed areas as in the two non-oiled areas. Larval growth rates were also severely affected; they were about half of those measured in populations from other areas of the north Pacific Ocean. A cautionary note was introduced to the effect that the differences in the egg-larval mortality between oiled and control sites may have been influenced by differences in egg dessication, predation, and wave scouring, hence these results should not be construed as conclusive evidence of oil spill damage. 40 refs., 6 figs

  4. Genetic and environmental correlates of morphological variation in a marine fish: the case of Baltic Sea herring ( Clupea harengus )

    DEFF Research Database (Denmark)

    Jørgensen, H.B.H.; Pertoldi, C.; Hansen, Michael Møller

    2008-01-01

    Baltic Sea herring (Clupea harengus) have been shown to exhibit morphological differences across the marked salinity and temperature gradients in the region. Here we analyse genetic (nine microsatellite loci), morpho metric (skull shape), and meristic (pectoral fin rays and number of vertebrae...... and plastic responses. Skull shape, including and excluding size variation, differed significantly among samples, both temporally and spatially. Genetic and morphometric distances were correlated, especially when size variation was excluded from the analysis. When size variation was included, skull shape...... variation was more closely correlated with environmental distances among spawning locations. Vertebrate number differed among samples and was correlated with environmental distances, whereas the number of fin rays was not. Genetic and geographic distances among samples were not correlated....

  5. The amount of food ingested in a single meal by rainbow trout offered chopped herring, dry and wet diets

    Science.gov (United States)

    Ruohonen; Grove; McIlroy

    1997-07-01

    Two-year-old 1·5-kg rainbow trout were held in cages and conditioned by feeding either on low-fat chopped herring (H trout) or dry pellets (P trout) for 15 weeks. Their satiation amounts were then determined under standard conditions. On a wet weight basis H trout ate 2·5-3·5 times more food than P trout; this was sufficient to compensate for the high water content of herring and thereby maintain the dry matter intake. When P trout were offered herring (PH trout) they consumed more food than when offered dry pellets but not as much as H trout. Stomach capacity restricted the intake and their dry matter intake was reduced by c. 40%. When H trout were offered dry pellets (HP trout) they adjusted their intake immediately close to the level of P trout although their larger stomachs could have accommodated more than twice this volume of dry food. The return of appetite after a satiation meal was almost linear with time. Appetite increased at c. 556 mg g-1 body weight h-1 for H trout and at 142 mg g-1 bw h-1 for P trout. The return of appetite in PH trout was significantly slower (c. 370 mg g-1 bw h-1) than in H trout; the previous dietary history of the PH trout limited their capacity to process larger volumes of wet food in a single meal. Fish offered dry diet (P and HP trout) had similar rates of appetite return despite their previous feeding history suggesting that the property of the dry feed itself might limit meal size. The total gastric emptying time of diets of similar dry matter content (with and without large amounts of water) was similar, but the delay time before gastric emptying starts tended to be longer for dry diets. Dry pellets appear to impose a demand for water that prolongs the gastric delay. This water demand is met partly by drinking since the trout fed on dry pellets drank significantly more (436±189 mg kg-1 h-1) than unfed and herring-fed trout which drank little or not at all (65±113 and 70±66 mg kg-1 h-1 respectively). Dietary water

  6. Effect of spatial differences in growth on distribution of seasonally co-occurring herring Clupea harengus stocks

    DEFF Research Database (Denmark)

    Worsøe Clausen, Lotte; Stæhr, Karl-Johan; Rindorf, Anna

    2015-01-01

    The mechanisms most likely to determine the distribution of the two major herring Clupea harengus stocks in their common early summer feeding ground in the eastern North Sea, Skagerrak and Kattegat were investigated through analysis of acoustic survey data from six consecutive years. No change...... was detected in biomass of North Sea autumn spawning C. harengus (NSAS) over time, whereas the biomass of western Baltic spring spawning C. harengus (WBSS) declined severely. Analyses of centre of abundance by stock showed no change in NSAS distribution, whereas the WBSS changed to a more western distribution...... Kattegat. The westward movement of spring spawners over time suggests that growth rate and possibly density of conspecifics influence the migration pattern and distribution of C. harengus in the area. In contrast, there was no evidence to suggest that distribution was constant over time within stocks...

  7. Scallop protein with endogenous high taurine and glycine content prevents high-fat, high-sucrose-induced obesity and improves plasma lipid profile in male C57BL/6J mice

    DEFF Research Database (Denmark)

    Tastesen, Hanne Sørup; Keenan, Alison H.; Madsen, Lise

    2014-01-01

    High-protein diets induce alterations in metabolism that may prevent diet-induced obesity. However, little is known as to whether different protein sources consumed at normal levels may affect diet-induced obesity and associated co-morbidities. We fed obesity-prone male C57BL/6J mice high-fat, high......-fed mice, but otherwise no changes in lean body mass were observed between the groups. Feed efficiency and apparent nitrogen digestibility were reduced in scallop-fed mice suggesting alterations in energy utilization and metabolism. Overnight fasted plasma triacylglyceride, non-esterified fatty acids......, glycerol and hydroxy-butyrate levels were significantly reduced, indicating reduced lipid mobilization in scallop-fed mice. The plasma HDL-to-total-cholesterol ratio was higher, suggesting increased reverse cholesterol transport or cholesterol clearance in scallop-fed mice in both fasted and non-fasted...

  8. Kinetics of viral load and erythrocytic inclusion body formation in pacific herring artificially infected with erythrocytic necrosis virus

    Science.gov (United States)

    Glenn, Jolene A.; Emmenegger, Eveline J.; Grady, Courtney A.; Roon, Sean R.; Gregg, Jacob L.; Conway, Carla M.; Winton, James R.; Hershberger, Paul K.

    2012-01-01

    Viral erythrocytic necrosis (VEN) is a condition that affects marine and anadromous fish species, including herrings and salmonids, in the Atlantic and Pacific oceans. Infection is frequently associated with severe anemia and causes episodic mortality among wild and hatchery fish when accompanied by additional stressors; VEN can be presumptively diagnosed by (1) light microscopic identification of a single characteristic—a round, magenta-colored, 0.8-μm-diameter inclusion body (IB) within the cytoplasm of erythrocytes and their precursors on Giemsa-stained blood films; or (2) observation (via transmission electron microscopy [TEM]) of the causative iridovirus, erythrocytic necrosis virus (ENV), within erythrocytes or their precursors. To better understand the kinetics of VEN, specific-pathogen-free Pacific herring Clupea pallasii were infected with ENV by intraperitoneal injection. At 1, 4, 7, 10, 14, 21, and 28 d postexposure, samples of blood, spleen, and kidney were collected and assessed (1) via light microscopy for the number of intracytoplasmic IBs in blood smears and (2) via TEM for the number of virions within erythrocytes. The mean prevalence of intracytoplasmic IBs in the blood cells increased from 0% at 0–4 d postexposure to 94% at 28 d postexposure. Viral load within circulating red blood cells peaked at 7 d postexposure, fell slightly, and then reached a plateau. However, blood cells observed within the kidney and spleen tissues demonstrated high levels of ENV between 14 and 28 d postexposure. The results indicate that the viral load within erythrocytes does not correlate well with IB prevalence and that the virus can persist in infected fish for more than 28 d.

  9. Impaired immune function in seals and laboratory rats exposed to dioxin-like compounds from Baltic herring

    Energy Technology Data Exchange (ETDEWEB)

    Ross, P.S. [Seal Rehabilitation and Research Centre, Pieterburen (Netherlands)]|[National Inst. of Public Health and Environmental Protection, Bilthoven (Netherlands); Swart, R.L. de [Seal Rehabilitation and Research Centre, Pieterburen (Netherlands)]|[Erasmus Univ., Rotterdam (Netherlands); Timmerman, H.H.; Loveren, H. van [National Inst. of Public Health and Environmental Protection, Bilthoven (Netherlands); Osterhaus, A.D.M.E. [Seal Rehabilitation and Research Centre, Pieterburen (Netherlands)]|[National Inst. of Public Health and Environmental Protection, Bilthoven (Netherlands)]|[Erasmus Univ., Rotterdam (Netherlands)

    1995-12-31

    Complex mixtures of lipophilic contaminants have been shown to affect certain top predators in the aquatic food chain, including seals. A recent demonstration that harbor seals (Phoca vitulina) fed Baltic Sea herring displayed impaired natural killer cell activity and T-lymphocyte function represented the first demonstration of immunotoxicity induced by ambient levels of contaminants in the environment. While these animals had a lower ability to respond to immunizations with inactivated vaccines, specific antibody responses, and in vitro antigen-specific lymphoproliferative responses, obvious constraints limited the ability to extend these results with host resistance tests or an evaluation of thymus and other lymphoid organs. The authors therefore set up a parallel study by exposing pregnant laboratory rats to the same Baltic herring contaminant mixture as received the seals. They then examined immune function parameters and host resistance to virus infection. As in the seals, rat pups of the Baltic group had impaired T-lymphocyte function. In addition, thymus cells and/or their precursors appeared to be targeted, as their numbers and function were reduced in the rats. Following challenge with rat cytomegalovirus in a host resistance study, rat pups in the Baltic group had impaired natural killer cell responses to the virus infection, and lower specific CD8 + (cytotoxic T-lymphocyte) responses following in vitro stimulation. By extrapolation, these results suggest that the impaired immune responses observed in the Baltic group of seals may lead to a less effective defense against virus infections in marine mammals inhabiting polluted coastal waters. Toxicological profiles and results of both the captive seal and laboratory rat experiments tend to implicate the 2,3,7,8-TCDD-like PCB, dioxin and furan congeners in the immunosuppression, and point to a major role for the PCBs.

  10. Molecular Cloning, Characterization, and Expression of a Catalase Gene in the Japanese Scallop Mizuhopecten yessoensis Induced in the Presence of Cadmium

    Science.gov (United States)

    Gao, Jialong; Ishizaki, Shoichiro; Nagashima, Yuji

    2016-03-01

    Cadmium (Cd) is known to influence the oxidative status of marine organisms and can induce the formation of reactive oxygen species (ROS). Catalase (CAT) is one of the important enzymes involved in scavenging high levels of ROS. In present study, we cloned CAT cDNA and investigated the response of this enzyme at the transcriptional level in the Japanese scallop Mizuhopecten yessoensis exposed to Cd. The full-length CAT cDNA (MyCAT) of 1,870 nucleotides including a 57 bp 5'-UTR, a coding sequence of 1,500 bp and a 313 bp 3'-UTR were identified from the scallop. The deduced amino acid sequence of MyCAT corresponds to 499 amino acids with predicted molecular weight of 56.48 kDa and contains highly conserved motifs of the proximal heme-binding site RLFSYSTH, proximal active signature FNRERIPERVVHAKGGG and three catalytic amino acid residues His72, Asn145, and Tyr355. Its significant homology to CATs from multiple alignments revealed that MyCAT had a high identity with CATs from other mollusks. CAT mRNA expression analysis revealed that expression level was highest in the digestive gland ( p < 0.01) but weak in muscle. Following exposure to 200 and 400 µg/l of Cd, a high amount of Cd was found to have accumulated in the digestive gland and CAT mRNA expression had significantly increased in this organ among 7-day exposed scallops ( p < 0.001). The result demonstrated that antioxidant enzymes such as CAT play important roles in counteracting Cd stress in M. yessoensis.

  11. Global phylogeography with mixed-marker analysis reveals male-mediated dispersal in the endangered scalloped hammerhead shark (Sphyrna lewini.

    Directory of Open Access Journals (Sweden)

    Toby S Daly-Engel

    Full Text Available The scalloped hammerhead shark, Sphyrna lewini, is a large endangered predator with a circumglobal distribution, observed in the open ocean but linked ontogenetically to coastal embayments for parturition and juvenile development. A previous survey of maternal (mtDNA markers demonstrated strong genetic partitioning overall (global Φ(ST = 0.749 and significant population separations across oceans and between discontinuous continental coastlines.We surveyed the same global range with increased sample coverage (N = 403 and 13 microsatellite loci to assess the male contribution to dispersal and population structure. Biparentally inherited microsatellites reveal low or absent genetic structure across ocean basins and global genetic differentiation (F(ST = 0.035 over an order of magnitude lower than the corresponding measures for maternal mtDNA lineages (Φ(ST = 0.749. Nuclear allelic richness and heterozygosity are high throughout the Indo-Pacific, while genetic structure is low. In contrast, allelic diversity is low while population structure is higher for populations at the ends of the range in the West Atlantic and East Pacific.These data are consistent with the proposed Indo-Pacific center of origin for S. lewini, and indicate that females are philopatric or adhere to coastal habitats while males facilitate gene flow across oceanic expanses. This study includes the largest sampling effort and the most molecular loci ever used to survey the complete range of a large oceanic predator, and findings emphasize the importance of incorporating mixed-marker analysis into stock assessments of threatened and endangered shark species.

  12. INTERESTERIFIKASI ENZIMATIS PALM STEARIN DAN MINYAK IKAN LEMURU UNTUK MEMBUAT LEMAK MARGARIN [Enzymatic Interesterification of Palm Stearin and Sardine oil to Produce Margarine-fat

    Directory of Open Access Journals (Sweden)

    Pudji Hastuti

    2003-04-01

    Full Text Available Enzymatic interesterification of Palm Stearin (PS and Sardine Oil (SO as source of Eicosa Pentaenoic Acid (EPA and Docosa Hexaenoic Acid (DHA have been of interest to modify the physical properties of the triglyceride. An attempt to enzymatic-restructure PS and SO to form Structured Lipid (SL which is suitable for margarine was investigated using immobilized lipase from Rhizomucor miehei and that from Candida antartica. The effect of reaction time course, ratio of PS/SO and ratio of enzyme/substrate were studied in the present study. At the end of interesterification, the enzyme was filtered from the reaction mixture through a filter paper. The Solid Fat Index (SFI was determined by dillatometry. The Slip Melting Point (SMP was determined by capillary tube method. Both of interesterification catalyzed by immobilized sn 1,3 specific lipase from R.miehei,and non specific lipase from C.antartica were found to decrease the SFI value at 10; 21.1 and 33.3°C. The SMP value was decrease from 58-50°C to 37-39°C. The change of these parameters were slightly faster in the reaction which catalyzed by lipase from R miehei than lipase from C.antartica . The more the utilization of the enzyme the faster the change were occurred, especially the increase of enzyme utilization from 2.5% to 5%, which decrease the SFI value at 33,30C. The decrease of the PS/SO ratio resulted in the decrease of SFI and SMP values. It was found that the most suitable SFI and SMP value for margarine fat is the SL formed by carrying out the enzymatic-interesterification of PS/SO with the ratio of 40/60 using enzyme 2.5% of the total fat, for 8 hours at 60°C.

  13. Hydro-plankton characteristics and their relationship with sardine and anchovy distributions on the French shelf of the Bay of Biscay

    Directory of Open Access Journals (Sweden)

    Pierre Petitgas

    2006-06-01

    Full Text Available The spatial pattern in hydro-plankton and fish distributions and their relationship were analysed based on the spring 2000 fisheries acoustic survey. The importance of this survey was that it was a multi-disciplinary platform which collected an extensive set of parameters in the hydro-plankton leading to a potentially finer description of hydro-plankton conditions and fish habitats. More than 50 variables were measured on a grid of stations, in four compartments of the ecosystem: hydrology, nutrients, primary producers and meso-zooplankton. First, a joint analysis of all hydro-plankton compartments was performed using multiple factor analysis (MFA. The method was used to estimate a compromise factorial space common to all compartments in which the stations were grouped by hierarchical clustering. The groups were represented spatially and a strong spatial pattern was evidenced. The fish and their spawned eggs were sampled along transect lines using acoustics and CUFES (continuous underway fish egg samplers. The distribution of the fish and their eggs was analysed in relation to the hydro-plankton groups of stations and difference in fish density across hydro-plankton conditions was tested by a pair-wise multiple comparison procedure. Anchovy was associated with a lesser number of hydro-plankton conditions than sardine. Eggs of both species were also associated with a lesser number of conditions than the fish. Finally, the gain provided by using the extensive set of hydro-plankton parameters for mapping large-scale hydro-plankton conditions was analysed in comparison with the situation in which a small set of parameters was available. The extensive set of parameters allowed more hydro-plankton conditions to be identified but only in the coastal area and not on the shelf. Size fractionated chlorophyll was determinant for tracking river plume hydro-plankton condition. However, the fish did not respond to the variety of the coastal hydro

  14. Protein hydrolysate from canned sardine and brewing by-products improves TNF-α-induced inflammation in an intestinal-endothelial co-culture cell model.

    Science.gov (United States)

    Vieira, Elsa F; Van Camp, John; Ferreira, Isabel M P L V O; Grootaert, Charlotte

    2017-07-17

    The anti-inflammatory activity of sardine protein hydrolysates (SPH) obtained by hydrolysis with proteases from brewing yeast surplus was ascertained. For this purpose, a digested and desalted SPH fraction with molecular weight lower than 10 kDa was investigated using an endothelial cell line (EA.hy926) as such and in a co-culture model with an intestinal cell line (Caco-2). Effects of SPH <10 kDa on nitric oxide (NO) production, reactive oxygen species (ROS) inhibition and secretion of monocyte chemoattractant protein 1 (MCP-1), vascular endothelial growth factor (VEGF), chemokine IL-8 (IL-8) and intercellular adhesion molecule-1 (ICAM-1) were evaluated in TNF-α-treated and untreated cells. Upon TNF-α treatment, levels of NO, MCP-1, VEGF, IL-8, ICAM-1 and endothelial ROS were significantly increased in both mono- and co-culture models. Treatment with SPH <10 kDa (2.0 mg peptides/mL) significantly decreased all the inflammation markers when compared to TNF-α-treated control. This protective effect was more pronounced in the co-culture model, suggesting that SPH <10 kDa Caco-2 cells metabolites produced in the course of intestinal absorption may provide a more relevant protective effect against endothelial dysfunction. Additionally, indirect cross-talk between two cell types was established, suggesting that SPH <10 kDa may also bind to receptors on the Caco-2 cells, thereby triggering a pathway to secrete the pro-inflammatory compounds. Overall, these in vitro screening results, in which intestinal digestion, absorption and endothelial bioactivity are simulated, show the potential of SPH to be used as a functional food with anti-inflammatory properties.

  15. Effect of sardine proteins on hyperglycaemia, hyperlipidaemia and lecithin:cholesterol acyltransferase activity, in high-fat diet-induced type 2 diabetic rats.

    Science.gov (United States)

    Benaicheta, Nora; Labbaci, Fatima Z; Bouchenak, Malika; Boukortt, Farida O

    2016-01-14

    Type 2 diabetes (T2D) is a major risk factor of CVD. The effects of purified sardine proteins (SP) were examined on glycaemia, insulin sensitivity and reverse cholesterol transport in T2D rats. Rats fed a high-fat diet (HFD) for 5 weeks, and injected with a low dose of streptozotocin, were used. The diabetic rats were divided into four groups, and they were fed casein (CAS) or SP combined with 30 or 5% lipids, for 4 weeks. HFD-induced hyperglycaemia, insulin resistance and hyperlipidaemia in rats fed HFD, regardless of the consumed protein. In contrast, these parameters lowered in rats fed SP combined with 5 or 30% lipids, and serum insulin values reduced in SP v. CAS. HFD significantly increased total cholesterol and TAG concentrations in the liver and serum, whereas these parameters decreased with SP, regardless of lipid intake. Faecal cholesterol excretion was higher with SP v. CAS, combined with 30 or 5% lipids. Lecithin:cholesterol acyltransferase (LCAT) activity and HDL3-phospholipids (PL) were higher in CAS-HF than in CAS, whereas HDL2-cholesteryl esters (CE) were lower. Otherwise, LCAT activity and HDL2-CE were higher in the SP group than in the CAS group, whereas HDL3-PL and HDL3-unesterified cholesterol were lower. Moreover, LCAT activity lowered in the SP-HF group than in the CAS-HF group, when HDL2-CE was higher. In conclusion, these results indicate the potential effects of SP to improve glycaemia, insulin sensitivity and reverse cholesterol transport, in T2D rats.

  16. Weight-length Relationships, Gonadosomatic Indeces, Sex Ratios and Relative Weight of the Omani-Indian Oil sardine, Sardinella longiceps (Valenciennes1847) from Al-Seeb Area; Sultanate of Oman

    OpenAIRE

    Al-Jufaili Saud

    2011-01-01

    This study presents the weight-length relationship and spawning period of the Omani Indian Oil sardine Sardinella longiceps (Valenciennes 1847) which inhabits the near shore zone along the Omani coast. A total of 1744 specimens were collected between January 1997 to February 1998 from Al-Seeb; a suburban area situated by the sea in Muscat, Oman. Samples were obtained on a monthly basis from beach seine and gillnet catches. The female to male sex ratio was obtained to be 0.6. The monthly varia...

  17. Radiocesium in marine fish, shrimps and scallops in coastal waters in Northern Norway and in the Barents Sea, measured over a period of two years

    International Nuclear Information System (INIS)

    Solberg, T.; Hellstroem, T.; Eikelmann, I.M.

    1995-01-01

    The paper deals with a national programme for assessing the radioactivity in marine species important for human consumption. The programme that started in 1993 and will continue for several years, focuses on the marine area of Norway north of the Arctic Circle. Using NaI detectors, measurements of 59 samples from fish, shrimps and scallops have recently been made. In all samples the radioactivity was below the detection limit (29 Bq/kg wet weight). Compared to the intervention levels for foodstuff, this level is negligible. 4 refs., 2 figs., 1 tab

  18. Monitoring of DSP toxins in small-sized plankton fraction of seawater collected in Mutsu Bay, Japan, by ELISA method: relation with toxin contamination of scallop.

    Science.gov (United States)

    Imai, Ichiro; Sugioka, Hikaru; Nishitani, Goh; Mitsuya, Tadashi; Hamano, Yonekazu

    2003-01-01

    Monitorings were conducted on DSP toxins in mid-gut gland of scallop (mouse assay), cell numbers of toxic dinoflagellate species of Dinophysis, and diarrhetic shellfish poisoning (DSP) toxins in small-sized (0.7-5 microm) plankton fraction of seawater collected from surface (0 m) and 20 m depth at a station in Mutsu Bay, Aomori Prefecture, Japan, in 2000. A specific enzyme-linked immunosorbent assay (ELISA) was employed for the analysis of DSP toxins in small-sized plankton fraction using a mouse monoclonal anti-okadaic acid antibody which recognizes okadaic acid, dinophysistoxin-1, and dinophysistoxin-3. DSP toxins were detected twice in the mid-gut gland of scallops at 1.1-2.3 MU (mouse units) g(-1) on 26 June and at 0.6-1.2 MU g(-1) on 3 July, respectively. Relatively high cell densities of D. fortii were observed on 26 June and 11 September, and may only contribute to the bivalve toxicity during late June to early July. D. acuminata did not appear to be responsible for the toxicity of scallops in Mutsu Bay in 2000. ELISA monitoring of small-sized plankton fraction in seawater could detect DSP toxins two weeks before the detection of the toxin in scallops, and could do so two weeks after the loss of the bivalve toxicity by mouse assay. On 17 July, toxic D. fortii was detected at only small number, <10 cells l(-1), but DSP toxins were detected by the ELISA assay, suggesting a presence of other toxic small-sized plankton in seawater. For the purpose of reducing negative impacts of DSP occurrences, monitorings have been carried out hitherto on DSP toxins of bivalve tissues by mouse assay and on cell densities of "toxic" species of Dinophysis. Here we propose a usefulness of ELISA monitoring of plankton toxicity, especially in small-sized fraction, which are possible foods of mixotrophic Dinophysis, as a practical tool for detecting and predicting DSPs in coastal areas of fisheries grounds of bivalve aquaculture.

  19. Examining the Effects of Chromatic Aberration, Object Distance, and Eye Shape on Image-Formation in the Mirror-Based Eyes of the Bay Scallop Argopecten irradians.

    Science.gov (United States)

    Speiser, Daniel I; Gagnon, Yakir Luc; Chhetri, Raghav K; Oldenburg, Amy L; Johnsen, Sönke

    2016-11-01

    The eyes of scallops form images using a concave spherical mirror and contain two separate retinas, one layered on top of the other. Behavioral and electrophysiological studies indicate that the images formed by these eyes have angular resolutions of about 2°. Based on previous ray-tracing models, it has been thought that the more distal of the two retinas lies near the focal point of the mirror and that the proximal retina, positioned closer to the mirror at the back of the eye, receives light that is out-of-focus. Here, we propose three mechanisms through which both retinas may receive focused light: (1) chromatic aberration produced by the lens may cause the focal points for longer and shorter wavelengths to fall near the distal and proximal retinas, respectively; (2) focused light from near and far objects may fall on the distal and proximal retinas, respectively; and (3) the eyes of scallops may be dynamic structures that change shape to determine which retina receives focused light. To test our hypotheses, we used optical coherence tomography (OCT), a method of near-infrared optical depth-ranging, to acquire virtual cross-sections of live, intact eyes from the bay scallop Argopecten irradians Next, we used a custom-built ray-tracing model to estimate the qualities of the images that fall on an eye's distal and proximal retinas as functions of the wavelengths of light entering the eye (400-700 nm), object distances (0.01-1 m), and the overall shape of the eye. When we assume 550 nm wavelength light and object distances greater than 0.01 m, our model predicts that the angular resolutions of the distal and proximal retinas are 2° and 7°, respectively. Our model also predicts that neither chromatic aberration nor differences in object distance lead to focused light falling on the distal and proximal retinas simultaneously. However, if scallops can manipulate the shapes of their eyes, perhaps through muscle contractions, we speculate that they may be able

  20. Examination of body burden and taint for Iceland scallop (Chlamys islandica) and American plaice (Hippoglossoides platessoides) near the Terra Nova offshore oil development over ten years of drilling on the Grand Banks of Newfoundland, Canada

    Science.gov (United States)

    DeBlois, Elisabeth M.; Kiceniuk, Joe W.; Paine, Michael D.; Kilgour, Bruce W.; Tracy, Ellen; Crowley, Roger D.; Williams, Urban P.; Gregory Janes, G.

    2014-12-01

    This paper presents results of analyses of body burdens of metals and hydrocarbons, and taste tests for taint, in Iceland scallop and American plaice performed as part of the Environmental Effects Monitoring (EEM) program for the Terra Nova offshore oil development (Grand Banks of Newfoundland, Canada). Scallop and plaice were collected in a Study Area located within approximately 1 km of drill centres at Terra Nova and in a Reference Area located approximately 20 km from the development. Samples were collected in 1997 to establish a baseline, and from 2000 to 2010, during drilling periods. Scallop adductor muscle tissue was contaminated with >C10-C21 aliphatic hydrocarbons resembling the drilling fluid in the synthetic drilling mud (SBM) used at Terra Nova in 2000, 2002 and 2004, but contamination of adductor muscle was not noted in 2006, 2008 and 2010. The maximum concentration in muscle was 28 mg/kg wet weight, noted in 2002. Scallop viscera was contaminated with hydrocarbons resembling drilling fluid in SBMs near drill centres in all EEM years except 2010. Viscera contamination with >C10-C21 hydrocarbons gradually decreased from a maximum of 150 mg/kg in 2000, to a maximum of 27 mg/kg in 2008; all values were below the laboratory reporting detection limit of 15 mg/kg in 2010. Therefore, evidence from both muscle and viscera indicates a decrease in tissue hydrocarbon contamination in recent years. Barium, another major constituent in drilling muds, has not been noted in scallop adductor muscles at concentrations above the reporting detection limit, but barium was detected in viscera in baseline and EEM years. The maximum concentration of barium in viscera during baseline sampling was 8 mg/kg. The maximum concentration in EEM years (29 mg/kg) was noted in 2000. The maximum concentration in 2010 was 25 mg/kg. The concentration of metals other than barium in scallop tissues was similar between the Terra Nova Study Area and the Reference Area. Hydrocarbons

  1. The southern Benguela ecosystem supports a large and highly ...

    African Journals Online (AJOL)

    spamer

    the principal feeding mode of anchovy (James and ... Although both species consumed similar types of food (crustacean eggs and nauplii, and copepods), sardine generally ..... DE SILVA, S. S. 1973 — Food and feeding habits of the herring.

  2. Release of infectious cells from epidermal ulcers in Ichthyophonus sp.-infected Pacific herring (Clupea pallasii): evidence for multiple mechanisms of transmission.

    Science.gov (United States)

    Kocan, Richard M; Gregg, Jacob L; Hershberger, Paul K

    2010-04-01

    A common clinical sign of ichthyophoniasis in herring and trout is "sandpaper" skin, a roughening of the epidermis characterized by the appearance of small papules, followed by ulceration and sloughing of the epithelium; early investigators hypothesized that these ulcers might be a means of transmitting the parasite, Ichthyophonus sp., without the necessity of ingesting an infected host. We examined the cells associated with the epidermal lesions and confirmed that they were viable Ichthyophonus sp. cells that were readily released from the skin into the mucous layer and ultimately into the aquatic environment. The released cells were infectious when injected into the body cavity of specific-pathogen-free herring. Our hypothesis is that different mechanisms of transmission occur in carnivorous and planktivorous hosts: Planktonic feeders become infected by ingestion of ulcer-derived cells, while carnivores become infected by ingestion of whole infected fish.

  3. Release of infectious cells from epidermal ulcers in Ichthyophonus sp.–infected Pacific Herring (Clupea pallasii): Evidence for multiple mechanisms of transmission

    Science.gov (United States)

    Hershberger, Paul K.; Gregg, Jacob L.; Kocan, R.M.

    2010-01-01

    A common clinical sign of ichthyophoniasis in herring and trout is “sandpaper” skin, a roughening of the epidermis characterized by the appearance of small papules, followed by ulceration and sloughing of the epithelium; early investigators hypothesized that these ulcers might be a means of transmitting the parasite, Ichthyophonus sp., without the necessity of ingesting an infected host. We examined the cells associated with the epidermal lesions and confirmed that they were viable Ichthyophonus sp. cells that were readily released from the skin into the mucous layer and ultimately into the aquatic environment. The released cells were infectious when injected into the body cavity of specific-pathogen-free herring. Our hypothesis is that different mechanisms of transmission occur in carnivorous and planktivorous hosts: Planktonic feeders become infected by ingestion of ulcer-derived cells, while carnivores become infected by ingestion of whole infected fish.

  4. Sublethal effects of the Exxon Valdez oil spill on herring embryos and larvae: morphological, cytogenetic; and histopathological assessments, 1989-1991

    International Nuclear Information System (INIS)

    Hose, J. E.; Marty, G. D.; Hinton, D. E.

    1996-01-01

    Extensive sublethal damage assessments were carried out on Pacific herring larvae following the Exxon Valdez oil spill. Egg masses were collected from oiled and non-oiled areas during 1989 to 1991, and incubated to hatch. The hatched larvae were evaluated for morphological deformities, cytogenetic abnormalities, and histopathological lesions. In 1989, herring larvae from the oiled areas showed high incidence of morphological deformities and cytogenetic abnormalities compared to larvae from non-oiled areas. There was no evidence of histopathological lesions. Identical assessments in 1990 and 1991 revealed no detectable oil-related developmental and genetic effects. In contrast, there was a marked decrease in survival, suggesting that that genetic damage at heavily oiled stations combined with severe morphological malformations mitigated against survival through the stressful larval period. 40 refs., 1 tab.,8 figs

  5. Distribution, density and abundance of the western Baltic herring ( Clupea harengus ) in the Sound (ICES Subdivision 23) in relation to hydrographical features

    DEFF Research Database (Denmark)

    Nielsen, J. Rasmus; Lundgren, Bo; Jensen, T. F.

    2001-01-01

    Biomass and duration of the over-wintering period of the Rugen spring spawning herring stock (RHS) in the Sound (ICES Subdivison 23) were investigated as well as possible hydrographical factors affecting relative distribution and triggering southwards migration towards the spawning grounds. Monit....... Monitoring was performed during 27 surveys over a 6-year period (1993-1998). Abundance of 45-165 000 t in August-February, 560 000 t in March-May, and...

  6. Developing Baltic cod recruitment models I : Resolving spatial and temporal dynamics of spawning stock and recruitment for cod, herring, and sprat

    DEFF Research Database (Denmark)

    Köster, Fritz; Möllmann, C.; Neuenfeldt, Stefan

    2001-01-01

    The Baltic Sea comprises a heterogeneous oceanographic environment influencing the spatial and temporal potential for reproductive success of cod (Gadus morhua) and sprat (Sprattus sprattus) in the different spawning basins. Hence, to quantify stock and recruitment dynamics, it is necessary......-disaggregated multispecies virtual population analyses (MSVPA) were performed for interacting species cod, herring (Clupea harengus), and sprat in the different subdivisions of the Central Baltic. The MSVPA runs revealed distinct spatial trends in population abundance, spawning biomass, recruitment, and predation...

  7. Mixed effects of elevated pCO2 on fertilisation, larval and juvenile development and adult responses in the mobile subtidal scallop Mimachlamys asperrima (Lamarck, 1819.

    Directory of Open Access Journals (Sweden)

    Elliot Scanes

    Full Text Available Ocean acidification is predicted to have severe consequences for calcifying marine organisms especially molluscs. Recent studies, however, have found that molluscs in marine environments with naturally elevated or fluctuating CO2 or with an active, high metabolic rate lifestyle may have a capacity to acclimate and be resilient to exposures of elevated environmental pCO2. The aim of this study was to determine the effects of near future concentrations of elevated pCO2 on the larval and adult stages of the mobile doughboy scallop, Mimachlamys asperrima from a subtidal and stable physio-chemical environment. It was found that fertilisation and the shell length of early larval stages of M. asperrima decreased as pCO2 increased, however, there were less pronounced effects of elevated pCO2 on the shell length of later larval stages, with high pCO2 enhancing growth in some instances. Byssal attachment and condition index of adult M. asperrima decreased with elevated pCO2, while in contrast there was no effect on standard metabolic rate or pHe. The responses of larval and adult M. asperrima to elevated pCO2 measured in this study were more moderate than responses previously reported for intertidal oysters and mussels. Even this more moderate set of responses are still likely to reduce the abundance of M. asperrima and potentially other scallop species in the world's oceans at predicted future pCO2 levels.

  8. Evaluation of the effects of the metals Cd, Cr, Pb and their mixture on the filtration and oxygen consumption rates in catarina scallop, Argopecten ventricosus juveniles.

    Science.gov (United States)

    Sobrino-Figueroa, Alma S; Cáceres-Martinez, Carlos

    2014-01-01

    In this work, we evaluated the effect of sublethal concentrations ( LC25, LC10 and LC5) of cadmium, chromium, lead, and their mixture on the filtration rate and oxygen consumption rate of Catarina scallop, Argopecten ventricosus (Sowerby, 1842), juveniles, in order to evaluate the use of these biomarkers as a reliable tool in environmental monitoring studies, because these metals have been found at high levels in water and sediments in the Mexican Pacific systems. An inverse dose-response relationship was observed when metal concentration and exposure time increased, the filtration rate and oxygen consumption rate reduced. The physiological responses evaluated in this study were sufficiently sensitive to detect alterations in the organisms at 0.014 mg l(-1) Cd, 0.311 mg l(-1) Cr, 0.125 mg l(-1) Pb and 0.05 mg l(-1) Cd + Cr + Pb at 24 and 72 hrs. Cd showed the most drastic effect. The Catarina scallop juveniles were more sensitive to Cd, Cr and Pb as compared to other bivalves. The biomarkers evaluated are a reliable tool to carry out environmental monitoring studies.

  9. Yield potential and fruit quality of scallop squash (Cucurbita pepo L. var. patissonina Greb. f. radiata Nois. cultivars grown for processing

    Directory of Open Access Journals (Sweden)

    Eugeniusz Kołota

    2015-10-01

    Full Text Available Scallop squash fruits may be harvested at different stages of development. Small size fruits, intended both for the fresh market and the food industry, have the highest biological value. This study was conducted to determine the yield potential of scallop squash cultivars (‘Polo F1’, ‘Disco’, ‘Gagat’, ‘Okra’, ‘Sunny Delight F1’ grown on mulched (black polyethylene foil or black agrotextile or unmulched soil, and harvested when fruits reached a diameter of 3–6 cm. Based on 3-year average data, the highest marketable and early yield was produced by ‘Sunny Delight F1’. This cultivar is also appreciated by consumers for its yellow colored skin of fruits. Another advantage of this cultivar was its high nutritional value expressed by the highest content of dry matter, vitamin C, polyphenols and minerals (K, P, Ca, Mg, with limited tendency to nitrate accumulation. Among the other cultivars tested, ‘Okra’ showed high yield potential, ‘Gagat’ was a rich source of carotenoids, and ‘Disco’ – polyphenols. Black polyethylene foil and black agrotextile appeared to be equally effective mulching materials. The benefit from the application of mulch was a significant increase in yield without any change in the chemical composition of the fruit.

  10. A multi-biomarker approach in scallop Chlamys farreri to assess the impact of contaminants in Qingdao coastal area of China.

    Science.gov (United States)

    Guo, Ruiming; Pan, Luqing; Ji, Rongwang

    2017-08-01

    A multi-biomarker approach was carried out to classify the environmental quality and the adverse effects of contaminants on scallop Chlamys farreri. The scallops were collected from three sampling stations in Qingdao coastal area of China in March, May, August and October of 2015. A suite of environmental factors and biomarkers, including temperature, salinity, pH, the concentrations of polycyclic aromatic hydrocarbons (PAHs), tetrabromobisphenol A (TBBPA) and metals (Cr, Mn, Cu, Zn, Cd, Pb, As) in seawater and soft tissue, mRNA expression of aryl hydrocarbon receptor (AhR) and P-glycoprotein (P-gp), 7-ethoxyresorufin O-deethylase (EROD), glutathione-S-transferase (GST), uridine-diphosphate-glucuronyl-transferase (UGT), sulfotransferase (SULT), metallothionein (MT), Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), lipid peroxidation (LPO) and protein carbonyl (PC) contents and DNA strand breaks, were measured in the gill and digestive gland. The results showed that S2 was the most polluted while S1 was identified the least polluted. Despite the differentiation of pollution levels and environmental parameters the selected biomarkers responded efficiently to contaminants. Principal component analysis (PCA) revealed that EROD for PAHs, AhR for TBBPA, MT for Cr, Pb and Mn, LPO and PC for Zn were the effective biomarkers respectively. This study demonstrated that the application of multi-biomarker approach in conjunction with the traditional analysis of environmental parameters and contaminants provided valuable information in environmental risk assessment. Copyright © 2017 Elsevier Inc. All rights reserved.

  11. Inability to demonstrate fish-to-fish transmission of Ichthyophonus from laboratory infected Pacific herring Clupea pallasii to naïve conspecifics.

    Science.gov (United States)

    Gregg, J L; Grady, C A; Friedman, C S; Hershberger, P K

    2012-06-13

    The parasite Ichthyophonus is enzootic in many marine fish populations of the northern Atlantic and Pacific Oceans. Forage fishes are a likely source of infection for higher trophic level predators; however, the processes that maintain Ichthyophonus in forage fish populations (primarily clupeids) are not well understood. Lack of an identified intermediate host has led to the convenient hypothesis that the parasite can be maintained within populations of schooling fishes by waterborne fish-to-fish transmission. To test this hypothesis we established Ichthyophonus infections in Age-1 and young-of-the-year (YOY) Pacific herring Clupea pallasii (Valenciennes) via intraperitoneal (IP) injection and cohabitated these donors with naïve conspecifics (sentinels) in the laboratory. IP injections established infection in 75 to 84% of donor herring, and this exposure led to clinical disease and mortality in the YOY cohort. However, after cohabitation for 113 d no infections were detected in naïve sentinels. These data do not preclude the possibility of fish-to-fish transmission, but they do suggest that other transmission processes are necessary to maintain Ichthyophonus in wild Pacific herring populations.

  12. An investigation of endocrine disrupting effects and toxic mechanisms modulated by benzo[a]pyrene in female scallop Chlamys farreri

    Energy Technology Data Exchange (ETDEWEB)

    Tian, Shuangmei; Pan, Luqing, E-mail: panlq@ouc.edu.cn; Sun, Xiaohua

    2013-11-15

    Highlights: •B[a]P disturbed progesterone, 17β-estradiol and testosterone production in scallop. •B[a]P inhibited 3β-HSD, CYP17 and 17β-HSD expression after a 10-day exposure. •B[a]P of lower dose elevated AHR-CYP1A expression but high dose B[a]P inhibited them. •ER and vitellogenin transcription was consistent with AHR after B[a]P exposure. •B[a]P exposure induced relatively developmental delay and impairment of ovary. -- Abstract: The purpose of this study was to investigate the endocrine disrupting effects induced by benzo[a]pyrene (B[a]P) and explore the underlying mechanisms in mollusks. In this study, sexually mature female Chlamys farreri were exposed to benzo[a]pyrene for 10 days at four different concentrations as 0, 0.025, 0.5 and 10 μg/L. Sex steroids were identified and quantified by electrochemiluminescence immunoassay (ECLIA) method and results showed that exposure to B[a]P exerts great suppression on 17β-estradiol, testosterone production and disrupts progesterone levels in ovary. Transcription of genes were detected and measured by real-time RT-PCR. It showed that at day 10 B[a]P inhibited 3β-HSD, CYP17 and 17β-HSD mRNA expression in a dose-dependent manner, which suggests that they could be potential targets of B[a]P that disrupt steroidogenic machinery. Moreover, 0.025 μg/L B[a]P activated transcription of aryl hydrocarbon receptor (AHR), AHR nuclear translocator (ARNT), CYP1A1 and estrogen receptor (ER), while 10 μg/L B[a]P suppressed all of them. The consistency of their responses to B[a]P exposure implies that AHR action may be involved in invertebrate CYP regulation and ER transcription despite of unknown mechanisms. Additionally, B[a]P exposure could induce ovarian impairment and developmental delay in C. farreri. Overall, sensitivity of C. farreri to endocrine disruption and toxicity suggests that C. farreri is a suitable species for study of endocrine-disrupting effects in marine invertebrates. This study will form a

  13. Effect of increased pCO2 level on early shell development in great scallop (Pecten maximus Lamarck larvae

    Directory of Open Access Journals (Sweden)

    S. Andersen

    2013-10-01

    Full Text Available As a result of high anthropogenic CO2 emissions, the concentration of CO2 in the oceans has increased, causing a decrease in pH, known as ocean acidification (OA. Numerous studies have shown negative effects on marine invertebrates, and also that the early life stages are the most sensitive to OA. We studied the effects of OA on embryos and unfed larvae of the great scallop (Pecten maximus Lamarck, at pCO2 levels of 469 (ambient, 807, 1164, and 1599 μatm until seven days after fertilization. To our knowledge, this is the first study on OA effects on larvae of this species. A drop in pCO2 level the first 12 h was observed in the elevated pCO2 groups due to a discontinuation in water flow to avoid escape of embryos. When the flow was restarted, pCO2 level stabilized and was significantly different between all groups. OA affected both survival and shell growth negatively after seven days. Survival was reduced from 45% in the ambient group to 12% in the highest pCO2 group. Shell length and height were reduced by 8 and 15%, respectively, when pCO2 increased from ambient to 1599 μatm. Development of normal hinges was negatively affected by elevated pCO2 levels in both trochophore larvae after two days and veliger larvae after seven days. After seven days, deformities in the shell hinge were more connected to elevated pCO2 levels than deformities in the shell edge. Embryos stained with calcein showed fluorescence in the newly formed shell area, indicating calcification of the shell at the early trochophore stage between one and two days after fertilization. Our results show that P. maximus embryos and early larvae may be negatively affected by elevated pCO2 levels within the range of what is projected towards year 2250, although the initial drop in pCO2 level may have overestimated the effect of the highest pCO2 levels. Future work should focus on long-term effects on this species from hatching, throughout the larval stages, and further into the

  14. An investigation of endocrine disrupting effects and toxic mechanisms modulated by benzo[a]pyrene in female scallop Chlamys farreri

    International Nuclear Information System (INIS)

    Tian, Shuangmei; Pan, Luqing; Sun, Xiaohua

    2013-01-01

    Highlights: •B[a]P disturbed progesterone, 17β-estradiol and testosterone production in scallop. •B[a]P inhibited 3β-HSD, CYP17 and 17β-HSD expression after a 10-day exposure. •B[a]P of lower dose elevated AHR-CYP1A expression but high dose B[a]P inhibited them. •ER and vitellogenin transcription was consistent with AHR after B[a]P exposure. •B[a]P exposure induced relatively developmental delay and impairment of ovary. -- Abstract: The purpose of this study was to investigate the endocrine disrupting effects induced by benzo[a]pyrene (B[a]P) and explore the underlying mechanisms in mollusks. In this study, sexually mature female Chlamys farreri were exposed to benzo[a]pyrene for 10 days at four different concentrations as 0, 0.025, 0.5 and 10 μg/L. Sex steroids were identified and quantified by electrochemiluminescence immunoassay (ECLIA) method and results showed that exposure to B[a]P exerts great suppression on 17β-estradiol, testosterone production and disrupts progesterone levels in ovary. Transcription of genes were detected and measured by real-time RT-PCR. It showed that at day 10 B[a]P inhibited 3β-HSD, CYP17 and 17β-HSD mRNA expression in a dose-dependent manner, which suggests that they could be potential targets of B[a]P that disrupt steroidogenic machinery. Moreover, 0.025 μg/L B[a]P activated transcription of aryl hydrocarbon receptor (AHR), AHR nuclear translocator (ARNT), CYP1A1 and estrogen receptor (ER), while 10 μg/L B[a]P suppressed all of them. The consistency of their responses to B[a]P exposure implies that AHR action may be involved in invertebrate CYP regulation and ER transcription despite of unknown mechanisms. Additionally, B[a]P exposure could induce ovarian impairment and developmental delay in C. farreri. Overall, sensitivity of C. farreri to endocrine disruption and toxicity suggests that C. farreri is a suitable species for study of endocrine-disrupting effects in marine invertebrates. This study will form a

  15. Seasonal variation in biomarkers in blue mussel (Mytilus edulis), Icelandic scallop (Chlamys islandica) and Atlantic cod (Gadus morhua)—Implications for environmental monitoring in the Barents Sea

    International Nuclear Information System (INIS)

    Nahrgang, J.; Brooks, S.J.; Evenset, A.; Camus, L.; Jonsson, M.; Smith, T.J.; Lukina, J.; Frantzen, M.; Giarratano, E.; Renaud, P.E.

    2013-01-01

    In the Barents Sea, the limited data on biological relevant indicators and their responses to various anthropogenic stressors have hindered the development of a consistent scientific basis for selecting indicator species and developing practical procedures for environmental monitoring. Accordingly, the main aim of the present study was to develop a common set of baseline values for contaminants and biomarkers in three species, and to identify their strengths and limitations in monitoring of the Barents Sea. Blue mussel (Mytilus edulis), Icelandic scallop (Chlamys islandica) and Atlantic cod (Gadus morhua) were sampled from a north Norwegian fjord in March, June, September and December 2010. Digestive glands from the bivalve species and liver from Atlantic cod were analysed for biomarkers of oxidative stress (catalase [CAT], glutathione peroxidase [GPX], glutathione-S-transferase activities [GST], lipid peroxidation as thiobarbituric reactive substances [TBARS] and total oxyradical scavenging capacity [TOSC]), biotransformation (ethoxyresorufine-O-deethylase activity [EROD]) and general stress (lysosomal membrane stability [LMS]). Concentrations of polycyclic aromatic hydrocarbons (PAHs) and metals in the bivalves and PAH metabolites in fish bile were quantified. Finally, energy reserves (total lipids, proteins and carbohydrates) and electron transport system (ETS) activity in the digestive gland of the bivalves and liver of Atlantic cod provided background information for reproductive cycle and general physiological status of the organisms. Blue mussel and Icelandic scallop showed very similar trends in biological cycle, biomarker expression and seasonality. Biomarker baselines in Atlantic cod showed weaker seasonal variability. However, important biological events may have been undetected due to the large time intervals between sampling occasions. Physiological biomarkers such as energy reserves and ETS activity were recommended as complementary parameters to the

  16. Seasonal variation in biomarkers in blue mussel (Mytilus edulis), Icelandic scallop (Chlamys islandica) and Atlantic cod (Gadus morhua)-Implications for environmental monitoring in the Barents Sea

    Energy Technology Data Exchange (ETDEWEB)

    Nahrgang, J., E-mail: jasmine.m.nahrgang@uit.no [Akvaplan-niva, FRAM Centre, NO-9296 Tromso (Norway); Brooks, S.J. [Norwegian Institute for Water Research (NIVA), NO-0349 Oslo (Norway); Evenset, A. [Akvaplan-niva, FRAM Centre, NO-9296 Tromso (Norway); Camus, L. [Akvaplan-niva, FRAM Centre, NO-9296 Tromso (Norway); University Centre In Svalbard (UNIS), NO-9171 Longyearbyen (Norway); Jonsson, M.; Smith, T.J. [Akvaplan-niva, FRAM Centre, NO-9296 Tromso (Norway); Lukina, J. [Akvaplan-niva, FRAM Centre, NO-9296 Tromso (Norway); University of Tromso, Faculty of Biosciences, Fisheries and Economics, Department of Arctic and Marine Biosciences, NO-9037 Tromso (Norway); Frantzen, M. [Akvaplan-niva, FRAM Centre, NO-9296 Tromso (Norway); Giarratano, E. [Centro Nacional Patagonico (CENPAT-CONICET), AR-9120 Puerto Madryn (Argentina); Renaud, P.E. [Akvaplan-niva, FRAM Centre, NO-9296 Tromso (Norway); University Centre In Svalbard (UNIS), NO-9171 Longyearbyen (Norway)

    2013-02-15

    In the Barents Sea, the limited data on biological relevant indicators and their responses to various anthropogenic stressors have hindered the development of a consistent scientific basis for selecting indicator species and developing practical procedures for environmental monitoring. Accordingly, the main aim of the present study was to develop a common set of baseline values for contaminants and biomarkers in three species, and to identify their strengths and limitations in monitoring of the Barents Sea. Blue mussel (Mytilus edulis), Icelandic scallop (Chlamys islandica) and Atlantic cod (Gadus morhua) were sampled from a north Norwegian fjord in March, June, September and December 2010. Digestive glands from the bivalve species and liver from Atlantic cod were analysed for biomarkers of oxidative stress (catalase [CAT], glutathione peroxidase [GPX], glutathione-S-transferase activities [GST], lipid peroxidation as thiobarbituric reactive substances [TBARS] and total oxyradical scavenging capacity [TOSC]), biotransformation (ethoxyresorufine-O-deethylase activity [EROD]) and general stress (lysosomal membrane stability [LMS]). Concentrations of polycyclic aromatic hydrocarbons (PAHs) and metals in the bivalves and PAH metabolites in fish bile were quantified. Finally, energy reserves (total lipids, proteins and carbohydrates) and electron transport system (ETS) activity in the digestive gland of the bivalves and liver of Atlantic cod provided background information for reproductive cycle and general physiological status of the organisms. Blue mussel and Icelandic scallop showed very similar trends in biological cycle, biomarker expression and seasonality. Biomarker baselines in Atlantic cod showed weaker seasonal variability. However, important biological events may have been undetected due to the large time intervals between sampling occasions. Physiological biomarkers such as energy reserves and ETS activity were recommended as complementary parameters to the

  17. Biomineralization changes with food supply confer juvenile scallops (Argopecten purpuratus) resistance to ocean acidification, supplement to: Ramajo, L; Marba, Núria; Prado, Luis; Peron, Sophie; Lardies, Marco A; Rodriguez-Navarro, Alejandro; Vargas, C A; Lagos, Nelson A; Duarte, Carlos M (2016): Biomineralization changes with food supply confer juvenile scallops (Argopecten purpuratus) resistance to ocean acidification. Global Change Biology, 22(6), 2025-2037

    KAUST Repository

    Ramajo, L

    2016-01-01

    Future ocean acidification (OA) will affect physiological traits of marine species, with calcifying species being particularly vulnerable. As OA entails high energy demands, particularly during the rapid juvenile growth phase, food supply may play a key role in the response of marine organisms to OA. We experimentally evaluated the role of food supply in modulating physiological responses and biomineralization processes in juveniles of the Chilean scallop, Argopecten purpuratus, that were exposed to control (pH 8.0) and low pH (pH 7.6) conditions using three food supply treatments (high, intermediate, and low). We found that pH and food levels had additive effects on the physiological response of the juvenile scallops. Metabolic rates, shell growth, net calcification, and ingestion rates increased significantly at low pH conditions, independent of food. These physiological responses increased significantly in organisms exposed to intermediate and high levels of food supply. Hence, food supply seems to play a major role modulating organismal response by providing the energetic means to bolster the physiological response of OA stress. On the contrary, the relative expression of chitin synthase, a functional molecule for biomineralization, increased significantly in scallops exposed to low food supply and low pH, which resulted in a thicker periostracum enriched with chitin polysaccharides. Under reduced food and low pH conditions, the adaptive organismal response was to trade-off growth for the expression of biomineralization molecules and altering of the organic composition of shell periostracum, suggesting that the future performance of these calcifiers will depend on the trajectories of both OA and food supply. Thus, incorporating a suite of traits and multiple stressors in future studies of the adaptive organismal response may provide key insights on OA impacts on marine calcifiers.

  18. Interaction Mode between Inclusion Complex of Vitamin K3 with γ- Cyclodextrin and Herring-Sperm DNA.

    Science.gov (United States)

    Tang, Yan; Cai, Li; Xue, Kang; Wang, Chunling; Xiong, Xiaoli

    2016-05-03

    Methods including spectroscopy, electronic chemistry and thermodynamics were used to study the inclusion effect between γ-cyclodextrin (CD) and vitamin K3(K3), as well as the interaction mode between herring-sperm DNA (hsDNA) and γ-CD-K3 inclusion complex. The results from ultraviolet spectroscopic method indicated that VK3 and γ-CD formed 1:1 inclusion complex, with the inclusion constant Kf = 1.02 × 10(4) L/mol, which is based on Benesi-Hildebrand's viewpoint. The outcomes from the probe method and Scatchard methods suggested that the interaction mode between γ-CD-K3 and DNA was a mixture mode, which included intercalation and electrostatic binding effects. The binding constants were K (θ)25°C = 2.16 × 10(4) L/mol, and K(θ)37°C = 1.06 × 10(4) L/mol. The thermodynamic functions of the interaction between γ-CD-K3 and DNA were ΔrHm(θ) = -2.74 × 10(4) J/mol, ΔrSm(θ) = 174.74 J·mol(-1)K(-1), therefore, both ΔrHm(θ) (enthalpy) and ΔrSm(θ) (entropy) worked as driven forces in this action.

  19. Length correction for early-juvenile Brazilian herring Sardinella janeiro (Eigenmann, 1894 after preservation in formalin, ethanol and freezing

    Directory of Open Access Journals (Sweden)

    Joaquim N. S. Santos

    Full Text Available This work aims to quantify the variation in total length and body mass for the early-juvenile Brazilian herring Sardinella janeiro and to determine total length and body mass correction equation to allow fresh measures to be calculated from preserved ones. Fishes were randomly assigned to one of five preservation methods (freezing at - 20º C, 2.5% and 5% formalin, 70% and 95% ethanol, and measured for total length (TL and body mass (W before preservation, and on days 5, 15, 30, and 60 after storage. Significant reductions in total length and body mass occurred during the first 5 days after preservation and continued to contract significantly at a lesser rate through 30 days in most methods. Exceptions were shown for body mass in freezing and 5% formalin, where the greatest losses occurred after 30 days of preservation. The degree of shrinkage for total length and body mass was very much dependent on fish size, with smaller specimens shrinking more than larger ones. The fresh total length and body mass can be back-calculated using equations that describe the relationship between fresh and preserved individuals after 60 days storage for all methods except for body mass in freezing.

  20. Amino acid specific stable nitrogen isotope values in avian tissues: Insights from captive American kestrels and wild herring gulls

    Science.gov (United States)

    Hebert, Craig E.; Popp, B.N.; Fernie, K.J.; Ka'apu-Lyons, C.; Rattner, Barnett A.; Wallsgrove, N.

    2016-01-01

    Through laboratory and field studies, the utility of amino acid compound-specific nitrogen isotope analysis (AA-CSIA) in avian studies is investigated. Captive American kestrels (Falco sparverius) were fed an isotopically characterized diet and patterns in δ15N values of amino acids (AAs) were compared to those in their tissues (muscle and red blood cells) and food. Based upon nitrogen isotope discrimination between diet and kestrel tissues, AAs could mostly be categorized as source AAs (retaining baseline δ15N values) and trophic AAs (showing 15N enrichment). Trophic discrimination factors based upon the source (phenylalanine, Phe) and trophic (glutamic acid, Glu) AAs were 4.1 (muscle) and 5.4 (red blood cells), lower than those reported for metazoan invertebrates. In a field study involving omnivorous herring gulls (Larus argentatus smithsonianus), egg AA isotopic patterns largely retained those observed in the laying female’s tissues (muscle, red blood cells, and liver). Realistic estimates of gull trophic position were obtained using bird Glu and Phe δ15N values combined with β values (difference in Glu and Phe δ15N in primary producers) for aquatic and terrestrial food webs. Egg fatty acids were used to weight β values for proportions of aquatic and terrestrial food in gull diets. This novel approach can be applied to generalist species that feed across ecosystem boundaries.

  1. Influência da temperatura na reprodução e longevidade do predador Orius thyestes Herring (Hemiptera, Anthocoridae Influence of the temperature on reproduction and longevity of the predator Orius thyestes Herring (Hemiptera, Anthocoridae

    Directory of Open Access Journals (Sweden)

    Lívia Mendes Carvalho

    2005-09-01

    Full Text Available O desenvolvimento e a fecundidade das espécies de Orius são bastante influenciados por uma série de fatores, como as condições ambientais, e em particular, pela temperatura. Esse trabalho teve como objetivo avaliar a reprodução e a longevidade de Orius thyestes Herring 1966 em diferentes temperaturas, tendo como alimento, ovos de Anagasta kuehniella (Zeller, 1879. O experimento foi conduzido em câmaras climatizadas com temperaturas de 16, 19, 22, 25, 28, 31 ± 1ºC, UR de 70±10% e fotofase de 12horas. Efeito deletério da temperatura em O. thyestes foi obtido a 16ºC, na qual apenas 40% das ninfas atingiram a fase adulta, e destes apenas 19% não apresentaram deformações morfológicas. O maior período de pré-oviposição foi observado a 19ºC (17,8 dias. Os maiores valores para a fecundidade média total foram registrados a 25 e 28ºC, com 109,2 e 128,2 ovos/fêmea, respectivamente, e o menor a 19ºC, com 22,8 ovos/fêmea. A 22 e 31ºC as fêmeas viveram mais que os machos, sendo que a 19ºC a longevidade foi maior, independente do sexo. As baixas temperaturas influenciaram a reprodução e longevidade de O. thyestes sugerindo que esta espécie poderá ter melhor performance reprodutiva em temperaturas mais elevadas, como aquelas de regiões tropicais e ou subtropicais.The development time and the fecundity of Orius species are strongly influenced by a wide range of factors, like environmental conditions and in particular temperature. This work aimed to evaluate the reproduction and adult longevity of Orius thyestes Herring, 1966 in different temperatures. Eggs of Anagasta kuehniella (Zeller, 1879 was provide as food supply. The experiment was carried out in climatic chambers at temperatures 16, 19, 22, 25, 28 and 31 ± 1°C, RH 70 ±10% and photophase 12h. A deleterious effect of the temperature on O. thyestes was found at 16°C, in which only 40% of the nymphs reached adulthood, of which only 19% presented normal morphological

  2. Results of the radiological verification survey at the former Herring-Hall-Marvin Safe Company, 1550 Grand Boulevard, Hamilton Ohio (HO001V)

    International Nuclear Information System (INIS)

    Murray, M.E.; allred, J.F.; Johnson, C.A.

    1995-11-01

    During the period between the 1940s and early 1950s, the Herring-Hall-Marvin Safe Company, 1550 Grand Boulevard, Hamilton, Ohio, was one company under subcontract to the Manhattan Engineer District (MED), and the Atomic Energy Commission (AEC), the lead agencies in the development of nuclear energy for defense-related projects. The US Department of Energy (DOE) conducted radiological surveys of these sites to evaluate current radiological conditions as part of the Formerly Utilized Sites Remedial Action Program (FUSRAP). In 1988, a radiological survey of the Herring-Hall-Marvin Safe Company facility was conducted, and after small fragments of uranium metal were removed, no beta or gamma radiation above background was detected and the building was dismissed from any additional DOE restrictions. In 1993, it was discovered that a portion of the actual machining work was conducted on the third floor of the facility, located in the southeastern comer of the building. At the request of DOE, this part of the facility was radiologically surveyed by an ORNL survey team to determine whether fixed surface contamination could be found that might exceed the DOE guidelines. Results of this radiological survey indicated 238 U contamination in excess of the DOE criteria for surface contamination, and the site was recommended for remediation. In February and March of 1995, a verification survey of the third floor of the former Herring-Hall-Marvin Safe Company facility by an ORNL survey team was performed in conjunction with decontamination operations conducted under the supervision of Bechtel National Incorporated. The verification survey included gamma scans at the surface and at one meter, alpha and beta-gamma scans for fixed contamination, and smears for transferable contamination

  3. A field and laboratory assessment of oil spill effects on survival and reproduction of Pacific herring following the Exxon Valdez spill

    International Nuclear Information System (INIS)

    Pearson, W.H.; Moksness, E.

    1995-01-01

    Field and laboratory investigations in 1989 and 1990 were designed to assess potential injury to Prince William Sound herring by testing for differences between oiled regions and unoiled reference areas and by relating biological response variables to the concentrations of polycyclic aromatic hydrocarbons (PAH) in eggs-on-kelp samples. Hydrocarbon analyses and laboratory incubation were conducted on eggs-on-kelp samples from Prince William Sound and Sitka Sound. The eggs and hatching larvae were examined to evaluate several response variables: egg development, hatch, larval survival, abnormal development of larvae, larval length, and larval yolk-sac volume. Analysis of 1989 shoreline surveys indicate that about 96% of the total spawn length (158 km) in Prince William Sound occurred along shorelines with no oiling, and less than 1% of the 1989 total spawn length occurred along shorelines with moderate to heavy oiling. Analysis of shoreline oiling in both 1989 and 1990 from all surveys indicates that about 90 to 91% of the total 1989 spawn length occurred along unoiled shorelines. Effects on herring eggs were minor in 1989 even in oiled areas. No significant relationship was found between 1989 PAH burdens in eggs-on-kelp samples and 9 out of 10 biological response variables. In 1989, significantly lower proportions of developed eggs were observed for Cabin Bay samples visibly contaminated with tarry deposits. The location where these effects were seen represented less than 2% of total 1989 spawn length. No effects of the spill on herring were evident in 1990. No significant relationship was found between 1990 PAH burdens and the seven biological response variables studied. 33 refs., 11 figs., 5 tabs

  4. Viral hemorrhagic septicemia virus, Ichthyophonus hoferi, and other causes of morbidity in Pacific herring Clupea pallasi spawning in Prince William Sound, Alaska, USA.

    Science.gov (United States)

    Marty, G D; Freiberg, E F; Meyers, T R; Wilcock, J; Farver, T B; Hinton, D E

    1998-02-26

    Pacific herring Clupea pallasi populations in Prince William Sound, Alaska, USA, declined from an estimated 9.8 x 10(7) kg in 1992 to 1.5 x 10(7) kg in 1994. To determine the role of disease in population decline, 233 Pacific herring from Prince William Sound were subjected to complete necropsy during April 1994. The North American strain of viral hemorrhagic septicemia virus (VHSV) was isolated from 11 of 233 fish (4.7%). VHSV was significantly related to myocardial mineralization, hepatocellular necrosis, submucosal gastritis, and meningoencephalitis. Ichthyophonus hoferi infected 62 of 212 (29%) fish. I. hoferi infections were associated with severe, disseminated, granulomatous inflammation and with increased levels of plasma creatine phosphokinase (CPK) and aspartate aminotransferase (AST). I. hoferi prevalence in 1994 was more than double that of most previous years (1989 to 1993). Plasma chemistry values significantly greater (p < 0.01) in males than females included albumin, total protein, cholesterol, chloride, glucose, and potassium; only alkaline phosphatase was significantly greater in females. Hypoalbuminemia was relatively common in postspawning females; other risk factors included VHSV and moderate or severe focal skin reddening. Pacific herring had more than 10 species of parasites, but they were not associated with significant lesions. Two of the parasites have not previously been described: a renal intraductal myxosporean (11% prevalence) and an intestinal coccidian (91% prevalence). Transmission electron microscopy of a solitary mesenteric lesion revealed viral particles consistent with lymphocystis virus. No fish had viral erythrocytic necrosis (VEN). Prevalence of external gross lesions and major parasites was not related to fish age, and fish that were year-lings at the time of the 1989 'Exxon Valdez' oil spill (1988 year class) had no evidence of increased disease prevalence.

  5. Between-year variability in the mixing of North Sea herring spawning components leads to pronounced variation in the composition of the catch

    DEFF Research Database (Denmark)

    Bierman, Stijn M.; Dickey-Collas, Mark; van Damme, Cindy J.G.

    2010-01-01

    North Sea herring (Clupea harengus) are managed as a single stock, but maintaining a diversity of spawning components is considered important. However, the total catch from each of these components cannot be estimated easily because the components mix during the summer feeding season. The spawning......-at-age between spawning components, in particular of the 2000 year class, may have caused the observed between-year changes in mixing of components. Our results indicate that estimates of compositions change when assumptions of perfect spatial mixing and perfect classification are relaxed, and can be uncertain...

  6. Disentangling the effects of evolutionary, demographic, and environmental factors influencing genetic structure of natural populations: Atlantic herring as a case study

    DEFF Research Database (Denmark)

    Gaggiotti, Oscar E.; Bekkevold, Dorte; Jørgensen, Hanne B.H.

    2009-01-01

    , on the other hand, seems to have been largely eroded, which is not surprising given the large reproductive potential and presumed enormous local effective population sizes of pelagic fish that constrain the effect of stochastic processes. The approach we used can in principle be applied to any abundant...... carried out separate analyses of neutral and selected genetic variation, which allowed us to establish that the two most important factors affecting population structure were selection due to salinity at spawning sites and feeding migrations. The genetic signal left by the demographic history of herring...

  7. Quantification of trypsin with a radioimmunoassay in herring larvae (Clupea harengus L.) compared with a highly sensitive fluorescence technique to determine tryptic enzyme activity

    International Nuclear Information System (INIS)

    Ueberschaer, B.; Pedersen, B.H.; Hjelmeland, K.

    1993-01-01

    Enzymatic activity and quantity of the protease trypsin were measured in individual herring larvae (Clupea harengus L.). The enzymatic activity assay was done by a fluorescence technique, and a radioimmunoassay was used for quantification of trypsin. The results are compared and the differences between the techniques discussed. Both methods have similar results, as high or low values in trypsin quantity were reflected in high or low values of tryptic activity. Quantity and activity were linearly and positively correlated, but small differences between methods were found at the lowest detection limits. Both techniques reflect the high variability between individual larvae. (orig.)

  8. Comparison of spatial and temporal trends of methoxylated PBDEs, PBDEs, and hexabromocyclododecane in herring along the Swedish coast

    Energy Technology Data Exchange (ETDEWEB)

    Asplund, L.; Nylund, K. [ITM, Stockholm Univ. (Sweden); Bignert, A. [Swedish Museum of Natural History, Stockholm (Sweden)

    2004-09-15

    Some brominated flame retardants, for example poly brominated diphenyl ethers (PBDEs) have been identified as ubiquitous environmental contaminants. The use of the technical PentaBDE product in the European Union has been drastically reduced in the last years and is banned from 2004, while another brominated flame retardant, hexabromocyclododecane (HBCD), continues to be used. In addition to the commercial BFR products, methoxylated PBDEs (MeO-PBDEs) have been identified in biota in the aquatic environment. The origin of the MeO-PBDEs is under discussion. To our knowledge, neither OH-PBDEs nor MeO-PBDEs are commercially produced, nor have they been reported as impurities in brominated technical products or formed as byproducts in any industrial process. However, MeO-PBDEs has been reported to be natural products formed in the marine environment. As regards MeO-PBDEs detected in Baltic fish several factors points in the direction of a natural origin. On the other hand, certain MeO-PBDEs detected in environmental samples could also be metabolites/environmental transformation products of PBDEs. Knowledge of the spatial and temporal trends of the flame retardants in biota can be very useful in identifying sources of emission to the environment and in evaluating the impacts of voluntary restrictions / bans (PBDEs) and ongoing use (HBCD) on environmental contamination. In addition, this knowledge could provide valuable help in identifying the origin of the MeO-PBDEs. To this end, the Swedish Environmental Monitoring Programme on Contaminants in Biota (SEMPC) has incorporated these 3 substance groups into its monitoring of organic contaminants in herring (Clupea harengus). In this contribution we report on the results of the first 4 years.

  9. The effects of pollutants on osmotic and ionic regulation of herring (Clupea harengus L. ) embryos and larvae

    Energy Technology Data Exchange (ETDEWEB)

    Ramsay, N.C. (Aberdeen Univ. (United Kingdom))

    1991-01-01

    This thesis looks at how osmoregulatory processes function during early ontogeny of herring (Clupea harengus L.) and how these can be affected by pollutants. First, during embryo osmoregulatory ontogeny, two distinct stages occur. Until the completion of epiboly and closure of the yolk plug, the embryo must rely wholly on passive osmoregulation. From epiboly to hatching, the embryo is able to regulate its osmolality and increases its water content through a drinking mechanism similar to that of the adult fish. Increased water content is paralleled by increased levels of solutes. The protein level thereafter decreases with subsequent increased ninhydrin positive substances. Excess salts are excreted through cells thought to be classical chloride cells. During the [open quotes]active[close quotes] osmoregulatory stage, the embryo has developed true osmotic homeostasis, providing continuity until the development of gills, skin, gut, kidney of the adult. Second, exposing embryos and larvae to a number of different pollutants affected parameters important for osmotic and ionic homeostasis. The most consistent response is an increased whole body electrolyte content and decreased water content, resulting in elevated osmolality. Exposure to drilling muds containing high levels of petrogenic components causes water loss from the larvae associated with a decreased drinking rate. Subsequent exposure to individual oil constituents at sublethal levels cause changes resulting in higher whole body electrolyte concentrations. In larvae exposed in vivo to metal levels below the Environmental Quality Standard, changes occurred in epithelial permeability. Effects on electrolyte concentrations also occurred in embryos but the mechanism was unclear. The mixture of heavy metals exposed in vitro resulted in a lower concentration required to cause a 50% reduction in whole body homogenate Na[sup +]K[sup +]-ATPase activity than for any individual metal.

  10. Effects of herring gulls and great black-backed gulls on breeding piping plovers, South Monomoy Island, Massachusetts. Final Report

    Science.gov (United States)

    Keane, S.E.; Fraser, J.D.; Buckley, P.A.

    2002-01-01

    The large population of breeding herring gulls and great black-backed gulls on South Monomoy Island, Cape Cod, Massachusetts has been thought to negatively affect the breeding success of the threatened piping plover. Following the Piping Plover Recovery Plan's call for gull colonies to be removed from piping plover breeding sites, in 1996, the USFWS conducted gull removal on part of South Monomoy Island. We determined relative gull abundance on South Monomoy Island from 1998-2000 by counting gulls within 100-m radius plots located on the shoreline. We quantified piping plover behavior and habitat use by conducting instantaneous and 5-minute behavioral observations. We quantified characteristics of piping plover nesting habitat by measuring characteristics along random transects. We measured gull abundance, beach width, and prey abundance, and then used logistic regression to determine what habitat characteristics influenced piping plover nesting area selection. We monitored piping plover reproductive success and population fluctuations on South Monomoy Island. Gull abundance in the gull-removal area was lower than gull abundance in the reference area throughout the piping plover breeding season. The difference in gull abundance between the areas did not affect piping plover behavior, nest success, chick survival, or productivity. We found that gull removal did not result in an increased piping plover population on the island. In both management areas, prenesting plovers preferred to forage in moist substrate habitats. Wide backshore and open vegetation habitats characterized nesting areas. Broods spent most of their time foraging and preferred moist substrate habitats when available. Plovers were not prevented from occupying more suitable habitat by large gulls. Fewer large gulls were observed near prenesting plovers, plover nests, and plover broods than near random plots. Fewer large gulls were observed in plover nesting areas than in unused areas when the nesting

  11. Growth performance and survival of larval Atlantic herring, under the combined effects of elevated temperatures and CO2.

    Directory of Open Access Journals (Sweden)

    Michael Sswat

    Full Text Available In the coming decades, environmental change like warming and acidification will affect life in the ocean. While data on single stressor effects on fish are accumulating rapidly, we still know relatively little about interactive effects of multiple drivers. Of particular concern in this context are the early life stages of fish, for which direct effects of increased CO2 on growth and development have been observed. Whether these effects are further modified by elevated temperature was investigated here for the larvae of Atlantic herring (Clupea harengus, a commercially important fish species. Over a period of 32 days, larval survival, growth in size and weight, and instantaneous growth rate were assessed in a crossed experimental design of two temperatures (10°C and 12°C with two CO2 levels (400 μatm and 900 μatm CO2 at food levels mimicking natural levels using natural prey. Elevated temperature alone led to increased swimming activity, as well as decreased survival and instantaneous growth rate (Gi. The comparatively high sensitivity to elevated temperature in this study may have been influenced by low food levels offered to the larvae. Larval size, Gi and swimming activity were not affected by CO2, indicating tolerance of this species to projected "end of the century" CO2 levels. A synergistic effect of elevated temperature and CO2 was found for larval weight, where no effect of elevated CO2 concentrations was detected in the 12°C treatment, but a negative CO2 effect was found in the 10°C treatment. Contrasting CO2 effects were found for survival between the two temperatures. Under ambient CO2 conditions survival was increased at 12°C compared to 10°C. In general, CO2 effects were minor and considered negligible compared to the effect of temperature under these mimicked natural food conditions. These findings emphasize the need to include biotic factors such as energy supply via prey availability in future studies on interactive

  12. Ecosystem scale acoustic sensing reveals humpback whale behavior synchronous with herring spawning processes and re-evaluation finds no effect of sonar on humpback song occurrence in the Gulf of Maine in fall 2006.

    Directory of Open Access Journals (Sweden)

    Zheng Gong

    Full Text Available We show that humpback-whale vocalization behavior is synchronous with peak annual Atlantic herring spawning processes in the Gulf of Maine. With a passive, wide-aperture, densely-sampled, coherent hydrophone array towed north of Georges Bank in a Fall 2006 Ocean Acoustic Waveguide Remote Sensing (OAWRS experiment, vocalizing whales could be instantaneously detected and localized over most of the Gulf of Maine ecosystem in a roughly 400-km diameter area by introducing array gain, of 18 dB, orders of magnitude higher than previously available in acoustic whale sensing. With humpback-whale vocalizations consistently recorded at roughly 2000/day, we show that vocalizing humpbacks (i were overwhelmingly distributed along the northern flank of Georges Bank, coinciding with the peak spawning time and location of Atlantic herring, and (ii their overall vocalization behavior was strongly diurnal, synchronous with the formation of large nocturnal herring shoals, with a call rate roughly ten-times higher at night than during the day. Humpback-whale vocalizations were comprised of (1 highly diurnal non-song calls, suited to hunting and feeding behavior, and (2 songs, which had constant occurrence rate over a diurnal cycle, invariant to diurnal herring shoaling. Before and during OAWRS survey transmissions: (a no vocalizing whales were found at Stellwagen Bank, which had negligible herring populations, and (b a constant humpback-whale song occurrence rate indicates the transmissions had no effect on humpback song. These measurements contradict the conclusions of Risch et al. Our analysis indicates that (a the song occurrence variation reported in Risch et al. is consistent with natural causes other than sonar, (b the reducing change in song reported in Risch et al. occurred days before the sonar survey began, and (c the Risch et al. method lacks the statistical significance to draw the conclusions of Risch et al. because it has a 98-100% false-positive rate

  13. Scalloped a member of the Hippo tumor suppressor pathway controls mushroom body size in Drosophila brain by non-canonical regulation of neuroblast proliferation.

    Science.gov (United States)

    Rohith, Basavanahalli Nanjundaiah; Shyamala, Baragur Venkatanarayanasetty

    2017-12-15

    Cell proliferation, growth and survival are three different basic processes which converge at determining a fundamental property -the size of an organism. Scalloped (Sd) is the first characterised transcriptional partner to Yorkie (Yki), the downstream effector of the Hippo pathway which is a highly potential and evolutionarily conserved regulator of organ size. Here we have studied the hypomorphic effect of sd on the development of Mushroom Bodies (MBs) in Drosophila brain. We show that, sd non-function results in an increase in the size of MBs. We demonstrate that, sd regulation on MB size operates through multiple routes. Sd expressed in the differentiated MB neurons, imposes non-cell autonomous repression on the proliferation of MB precursor cells, and Sd expression in the MB neuroblasts (NB) cell autonomously represses mushroom body neuroblast (MBNB) proliferation. Further Sd in Kenyon cells (KCs) imparts a cell autonomous restriction on their growth. Our findings are distinctive because, while the classical sd loss of function phenotypes in eye, wing and lymph gland are reported as loss of tissue or reduced organ size, the present study shows that, Sd inactivation in the developing MB, promotes precursor cell proliferation and results in an increase in the organ size. Copyright © 2017 Elsevier Inc. All rights reserved.

  14. Site suitability analysis for Bay scallop aquaculture and implications for sustainable fisheries management in the Ha Long Bay archipelago, northern Vietnam

    Directory of Open Access Journals (Sweden)

    Pham Thi Khanh

    2013-01-01

    Full Text Available Mollusc culture if properly managed, may help decrease capture fisheries over-exploitation in Vietnam, and possibly become an alternative income for local fishermen. The definition and characterization of zones suitable for aquaculture is pivotal for its success and sustainable development, and this study aims at determining the suitability of Argopecten irradians (Bay scallop culture in the Ha Long Bay Archipelago. Temperature, salinity, chlorophyll-a, total suspended solid and bathymetry, were compiled in an environmental suitability model. Distance of culture sites from landing points and fish markets were instead grouped in an infrastructural suitability model. In both models, developed with Geographic Information Systems, the suitability scores were ranked on a scale from 1 (unsuitable to 6 (very-highly suitable. Results showed that 98 % of the studied area is environmentally suitable for such culture. However, overlaying the infrastructural factors the suitable zone decrease to 38 %. Advantages and disadvantages of two management options were then discussed: (a strengthening fisheries infrastructures or (b developing post harvesting processing plants.

  15. Effect of cadmium, chromium, lead and metal mixtures on survival and growth of juveniles of the scallop Argopecten ventricosus (Sowerby II, 1842).

    Science.gov (United States)

    Sobrino-Figueroa, Alma S; Cáceres-Martínez, Carlos; Botello, Alfonso V; Nunez-Nogueira, G

    2007-08-01

    The effects of Cd, Cr, Pb and their mixtures on the growth and sensitivity of the scallop Argopecten ventricosus were analyzed in this study. Cadmium showed to be more toxic metal to juveniles (96 hour median lethal concentration (LC(50)) = 0.396 mg Cd/L), followed by lead (LC(50) = 0.830 mg Pb/L) and chromium (LC(50) = 3.430 mg Cr/L). Cadmium toxicity was 8 times higher than chromium and 2 times than lead. The most toxic combination was Cd + Cr + Pb. (LC(50) = 0.302 mg/L). Based on toxic units analyses (T.U.), a synergistic effect was observed for Cr + Pb and Cd + Cr + Pb. (T.U. = 0.374; T.U. = 0.403), and antagonic effects for Cd + Cr and Cd + Pb (T.U. = 1.26; T.U. = 1.43) respectively. The level of effect (from high to low) on the growth of A. ventricosus juveniles was: Cd > Cd + Cr + Pb > Cr > Pb. The EC(50) (metal concentration where a reduction of 50% growing rate is observed) obtained were: Cd = 0.018 mg/L, Cd + Cr + Pb = 0.104 mg/L, Cr = 0.51 mg/L and Pb = 4.21 mg/L. These results suggest that A. ventricosus juveniles are more sensitive to these metals in comparison to other juveniles from other bivalve species (e.g., A. irradians, Mytillus edulis, Crassostrea virginica).

  16. Molecular cloning and responsive expression to injury stimulus of a defender against cell death 1 (DAD1) gene from bay scallops Argopecten irradians.

    Science.gov (United States)

    Zhu, Ling; Song, Linsheng; Zhang, Huan; Zhao, Jianmin; Li, Chenghua; Xu, Wei

    2008-06-01

    Apoptosis is an active process of cell death, which is an integral part of growth and development in multicellular organisms. The defender against cell death 1 (DAD1), the regulatory protein to inhibit the apoptosis process, was first cloned from the bay scallop Argopecten irradians by randomly sequencing a whole tissue cDNA library and rapid amplification of cDNA end (RACE). The full-length cDNA of the A. irradians DAD1 was 607 bp, consist of a 5'-terminal untranslated region (UTR) of 63 bp, a 3'-terminal UTR of 205 bp with a canonical polyadenylation signal sequence AATAAA and a poly (A) tail, and an open reading frame of 339 bp. The deduced amino acid sequence of the A. irradians DAD1 showed 75.5% identity to Araneus ventricosus, 74.5% to Drosophila melanogaster, and 73.6% to Homo sapiens, Sus scrofa, Mesocricetus auratus, Rattus norvegicus and Mus musculus. Excluding the Saccharomyces cerevisiae DAD1 homologue, all animal DAD1 including A. irradians DAD1 homologue formed a subgroup and all plant DAD1 proteins formed another subgroup in the phylogenetic analysis. The A. irradians DAD1 was expressed in all examined tissues including adductor muscle, mantle, gills, digestive gland, gonad and hemolymph, suggesting that A. irradians DAD1 is expressed in most body tissues. Furthermore, the mRNA expression levels of A. irradians DAD1 gene of hemolymph were particularly high after injury, suggesting that the gene is responsive to injury stimuli.

  17. Specific pathways for the incorporation of dissolved barium and molybdenum into the bivalve shell: an isotopic tracer approach in the juvenile Great Scallop (Pecten maximus).

    Science.gov (United States)

    Tabouret, Hélène; Pomerleau, Sébastien; Jolivet, Aurélie; Pécheyran, Christophe; Riso, Ricardo; Thébault, Julien; Chauvaud, Laurent; Amouroux, David

    2012-07-01

    Dissolved barium and molybdenum incorporation in the calcite shell was investigated in the Great Scallop Pecten maximus. Sixty six individuals were exposed for 16 days to two successive dissolved Ba and Mo concentrations accurately differentiated by two different isotopic enrichments (⁹⁷Mo, ⁹⁵Mo; ¹³⁵Ba, ¹³⁷Ba). Soft tissue and shell isotopic composition were determined respectively by quantitative ICP-MS (Inductively Coupled Plasma Mass Spectrometer) and laser ablation--ICP-MS. Results from Ba enrichment indicate the direct incorporation of dissolved Ba into the shell in proportion to the levels in the water in which they grew with a 6-8 day delay. The low spike contributions and the low partition coefficient (D(Mo) = 0.0049 ± 0.0013), show that neither the soft tissue nor the shell were significantly sensitive to Mo enrichment. These results eliminate direct Mo shell enrichment by the dissolved phase, and favour a trophic uptake that will be investigated using the successive isotopic enrichment approach developed in this study. Copyright © 2012 Elsevier Ltd. All rights reserved.

  18. Atlantic Herring Acoustic Surveys

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NEFSC Advanced Sampling Technologies Research Group conducts annual fisheries acoustic surveys using state-of-the-art acoustic, midwater trawling, and underwater...

  19. Red herring vaginal discharge.

    Science.gov (United States)

    Lee, Jun Hee; Pringle, Kirsty; Rajimwale, Ashok

    2013-09-18

    Labial hair tourniquet syndrome is a rare condition that can be easily misdiagnosed and ultimately lead to irreversible damage. An 11-year-old premenarche girl presented with a 5-day history of pain and swelling in the labia with associated vaginal discharge. The general practitioner treated her with clotrimazole without improvement. On examination, there was an oedematous swelling of the right labia with a proximal hair tourniquet. Local anaesthetic was applied and the hair removed with forceps. There was instant relief of pain and the discharge stopped within 24 h. The patient was sent home with a course of antibiotics.

  20. Argentina's nuclear red herring

    International Nuclear Information System (INIS)

    Perera, J.

    1983-01-01

    The article deals with Argentina's nuclear power programme over the last 30 years, including the country's efforts to be nuclear independent of the United States, as well as its aspirations to be a nuclear supplier to latin America. The latter policy on economic, rather than military grounds for nuclear weapons, has led to Argentina's decision to build a uranian enrichment plant. (U.K.)

  1. Effects of pressure reductions in a proposed siphon water lift system at St. Stephen Dam, South Carolina, on mortality rates of juvenile American shad and blueback herring. Technical report

    International Nuclear Information System (INIS)

    Nestler, J.M.; Schilt, C.R.; Jones, D.P.

    1998-09-01

    This report presents results of studies to predict the mortality rate of juvenile blueback herring (Alosa aestivalis) and American shad (A. sapidissima) associated with reduced pressure as they pass downstream through a proposed siphon water lift system at St. Stephen Dam, South Carolina. The primary function of the siphon is to increase attracting flow to better guide upstream migrating adult herring of both species into the existing fish lift for upstream passage. The US Army Engineer District, Charleston, wishes to consider the siphon as an alternative bypass route through the dam for downstream migrating juvenile and adult herring. A pressure-reduction testing system that emulates some of the pressure characteristics of the siphon was used to determine the approximate percentage of juvenile fishes that could be reasonably expected to be killed passing through the reduced pressures anticipated for the siphon water lift system. The testing system could duplicate the range of pressure change anticipated for the siphon lift system but could not obtain pressures lower than 4.1 psi, whereas pressures for some design alternatives may approach the theoretical minimum pressure of 0.0 psi. Study results indicate that the mortality rate is probably about 20 percent. Power analysis indicates that mortality rate above 30 percent is unlikely. Conducting additional mortality studies is recommended to refine predicted mortality rates. Measures should be taken to prevent juvenile fish from entering the siphon lift system if excessive mortality rates are observed

  2. Elimination of vibrio cholerae El Tor, in scallops (Argopecten purpuratus) by gamma radiation and product organoleptic evaluation; Eliminacion de vibrio cholerae, El Tor, en conchas de abanico (Argopecten purpuratus) con el uso de radiacion gamma y evaluacion sensorial del producto

    Energy Technology Data Exchange (ETDEWEB)

    Torres, Z [Instituto Peruano de Energia Nuclear, Lima (Peru); Bernuy, B [Universidad Nacional Agraria la Molina, Lima (Peru); Vivanco, M [Universidad Nacional Federico Villarreal, Lima (Peru); Kahn, G [Universidad Nacional Agraria de la Selva, Pucallpa (Peru)

    1999-03-01

    V. cholerae D{sub 10} value in scallops (Argopecten purpuratus) was determined in vivo. D{sub 10} was found to be 0,143 kGy, requiring therefore the application of 8D in scallops, equivalent to a 1,14 kGy dose, the optimal dose for life span extension of samples kept under refrigeration conditions (0-1{sup o}), and examined periodically under different analytic method criteria. Life span for the appearance characteristic reaches the acceptability limit of 3, after 11 days for control samples, and 16 and 13 days for samples radiated at 1 and 2 kGy. Smell of control samples was accepted only until the 13{sup th} day while samples radiated at 1 and 2 kGy went beyond this level, reaching 19 and 17 days respectively. In the same way, life span for the flavor characteristic was extended to 19 and 20 days for samples radiated at 1 and 2 kGy, respectively, while control samples only reached 15 days. Control sample texture remained within acceptable limits until the 18{sup th} day, whereas samples radiated at 1 and 2 kGy reached 21 and 17 days, respectively. Use of ph and nitrogen volatile bases were also evaluated as quality indicators. (authors) 14 refs., 3 figs., 3 tabs., 1 ill.

  3. Environmentally relevant organophosphate triesters in herring gulls: In vitro biotransformation and kinetics and diester metabolite formation using a hepatic microsomal assay

    International Nuclear Information System (INIS)

    Greaves, Alana K.; Su, Guanyong; Letcher, Robert J.

    2016-01-01

    The in vitro biotransformation and kinetics of six organophosphate triester (OPE) flame retardants were investigated in herring gulls (Larus argentatus) from the Great Lakes using a hepatic microsomal metabolism assay. Administration of each individual OPE (tri-n-butyl phosphate (TNBP), tris(2-butoxyethyl) phosphate (TBOEP), triphenyl phosphate (TPHP), triethyl phosphate (TEP), tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) and tris(2-chloroisopropyl) phosphate (TCIPP)) to the in vitro assay (concentration range 0.01 to 10 μM) resulted in rapid depletion with the exception of TEP. Following the Michaelis-Menten enzyme kinetics model, a preliminary 2-minute incubation period was used to estimate the V max (± SE) values (i.e., the maximal rate of reaction for a saturated enzyme system), which ranged from 5.0 ± 0.4 (TPHP) to 29 ± 18 pmol/min/mg protein (TBOEP), as well as the K M (± SE) values (i.e., the OPE concentration corresponding to one half of the V max ), which ranged from 9.8 ± 1 (TPHP) to 189 ± 135 nM (TBOEP). Biotransformation assays over a 100-minute incubation period revealed that TNBP was metabolized most rapidly (with a depletion rate of 73 ± 4 pmol/min/mg protein), followed by TBOEP (53 ± 8 pmol/min/mg), TCIPP (27 ± 1 pmol/min/mg), TPHP (22 ± 2 pmol/min/mg) and TDCIPP (8 ± 1 pmol/min/mg). In vitro biotransformation of OP triesters was clearly structure-dependent where non-halogenated alkyl OP triesters were metabolized more rapidly than halogenated alkyl triesters. Halogenated OP triesters were transformed to their respective diesters more efficiently relative to non-halogenated OP triesters. To our knowledge, this is the first study to investigate OP triester metabolism and OP diester formation in an avian or wildlife model system, which is important to understand the fate and biological activity of OPEs in an exposed organism. - Highlights: • The metabolism and kinetics of 6 OPEs were examined in herring gull liver microsomes. • The

  4. Environmentally relevant organophosphate triesters in herring gulls: In vitro biotransformation and kinetics and diester metabolite formation using a hepatic microsomal assay

    Energy Technology Data Exchange (ETDEWEB)

    Greaves, Alana K. [Wildlife and Landscape Directorate, Science and Technology Branch, Environment and Climate Change Canada, National Wildlife Research Centre, Carleton University, Ottawa, ON K1A 0H3 (Canada); Department of Chemistry, Carleton University, Ottawa, ON K1S 5B6 (Canada); Su, Guanyong, E-mail: guanyong.su85@gmail.com [Wildlife and Landscape Directorate, Science and Technology Branch, Environment and Climate Change Canada, National Wildlife Research Centre, Carleton University, Ottawa, ON K1A 0H3 (Canada); Department of Chemistry, Carleton University, Ottawa, ON K1S 5B6 (Canada); Letcher, Robert J., E-mail: robert.letcher@canada.ca [Wildlife and Landscape Directorate, Science and Technology Branch, Environment and Climate Change Canada, National Wildlife Research Centre, Carleton University, Ottawa, ON K1A 0H3 (Canada); Department of Chemistry, Carleton University, Ottawa, ON K1S 5B6 (Canada)

    2016-10-01

    The in vitro biotransformation and kinetics of six organophosphate triester (OPE) flame retardants were investigated in herring gulls (Larus argentatus) from the Great Lakes using a hepatic microsomal metabolism assay. Administration of each individual OPE (tri-n-butyl phosphate (TNBP), tris(2-butoxyethyl) phosphate (TBOEP), triphenyl phosphate (TPHP), triethyl phosphate (TEP), tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) and tris(2-chloroisopropyl) phosphate (TCIPP)) to the in vitro assay (concentration range 0.01 to 10 μM) resulted in rapid depletion with the exception of TEP. Following the Michaelis-Menten enzyme kinetics model, a preliminary 2-minute incubation period was used to estimate the V{sub max} (± SE) values (i.e., the maximal rate of reaction for a saturated enzyme system), which ranged from 5.0 ± 0.4 (TPHP) to 29 ± 18 pmol/min/mg protein (TBOEP), as well as the K{sub M} (± SE) values (i.e., the OPE concentration corresponding to one half of the V{sub max}), which ranged from 9.8 ± 1 (TPHP) to 189 ± 135 nM (TBOEP). Biotransformation assays over a 100-minute incubation period revealed that TNBP was metabolized most rapidly (with a depletion rate of 73 ± 4 pmol/min/mg protein), followed by TBOEP (53 ± 8 pmol/min/mg), TCIPP (27 ± 1 pmol/min/mg), TPHP (22 ± 2 pmol/min/mg) and TDCIPP (8 ± 1 pmol/min/mg). In vitro biotransformation of OP triesters was clearly structure-dependent where non-halogenated alkyl OP triesters were metabolized more rapidly than halogenated alkyl triesters. Halogenated OP triesters were transformed to their respective diesters more efficiently relative to non-halogenated OP triesters. To our knowledge, this is the first study to investigate OP triester metabolism and OP diester formation in an avian or wildlife model system, which is important to understand the fate and biological activity of OPEs in an exposed organism. - Highlights: • The metabolism and kinetics of 6 OPEs were examined in herring gull liver

  5. Morphological alterations in cryopreserved spermatozoa of scallop Argopecten purpuratus Alteraciones morfológicas en espermatozoides criopreservados de concha de abanico Argopecten purpuratus

    Directory of Open Access Journals (Sweden)

    Carlos Espinoza

    2010-01-01

    Full Text Available The present work identifies and quantifies the morphological alterations of scallop Argopecten purpuratus spermatozoa caused by long-term cryopreservation. Percentages of motility, fertilization and injured spermatozoa were quantified by optic microscopy and scanned electron microscopy. These parameters were evaluated in sperm without treatment (CTR, spermatozoa incubated in cryoprotective solution but not freezed (ICS and freezed-thawed spermatozoa (FTS. Spermatozoa of ICS treatment remained motile longer than those of CTR, whereas those of FTS treatment were lowest. Morphology of the spermatozoa was affected in several ways by the freeze-thawing treatment; some had their head deformed or swollen, others had their cell membrane folded or broken; acrosome reaction; anomalous positions or absence of mitochondria as well as broken, stiff or loss of lineal structure of tail. CTR and ICS treatments had higher percentages of undamaged sperm (87.7% and 79.0% respectively, while FTS samples had 14.2% of undamaged sperm. The tail was the spermatic structure most commonly injured in FTS (77.0%, the percentage of sperm with head injury was 55.1% and with acrosome reaction was 28.7%, whereas middle piece was affected in 23.9% of sperm. Percentages of fertilization were 68.3%, 67.9% and 58.2% for CTR, ICS and FTS respectively, which were not significantly different. There was a higher correlation between injuries and motility than between injuries and fertilization success. Correlation between motility and fertilization was low (0.605 and 0.668 with motility at 5 and 30 min, respectively.El presente trabajo identifica y cuantifica las alteraciones morfológicas en espermatozoides de concha de abanico A. purpuratus causadas por la criopreservación en nitrógeno líquido. Porcentajes de motilidad, fecundación de ovocitos frescos y espermatozoides lesionados (en cabeza, acrosoma, pieza media y flagelo fueron determinados bajo microscopía óptica y electr

  6. Radium-226 equilibrium between water and lake herring, Coregonus artedii, tissues attained within fish lifetime: confirmation in this species of one assumption in the simple linear concentration factor model

    International Nuclear Information System (INIS)

    Clulow, F.V.; Pyle, G.G.

    1997-01-01

    Equilibrium conditions are assumed in the simple linear concentration factor model commonly used in simulations of contaminant flow through ecosystems and in dose and risk calculations. Predictions derived from a power function model have suggested that if the time scale of the food-chain transfer is less than six years in fish, radium-226 equilibrium will not be achieved in nature, thereby violating the equilibrium requirement in the concentration factor model. Our results indicate 226 Ra equilibrium is achieved in a natural population of lake herring (Coregonus artedii), contrary to predictions of the power function model. (author)

  7. Influência da ingestão de sardinha nos níveis de ácidos graxos poliinsaturados da série ômega3 no leite materno The influence of sardine consumption on the omega-3 fatty acid content of mature human milk

    Directory of Open Access Journals (Sweden)

    Rose V. Patin

    2006-02-01

    Full Text Available OBJETIVOS: A proposta deste trabalho foi verificar a influência da ingestão de sardinha, alimento rico em ácidos graxos poliinsaturados da série ômega3, na composição do leite materno. MÉTODOS: Estudo prospectivo avaliou 31 nutrizes acompanhadas no Hospital Guilherme Álvaro, as quais receberam 2 kg de sardinha fresca por duas vezes, em intervalos de 15 dias. Nos tempos 0, 15 e 30 dias, realizou-se inquérito alimentar de 24 horas e coleta de leite. Determinaram-se os ácidos graxos do leite materno por cromatografia a gás. Para análise estatística dos resultados, utilizaram-se testes não paramétricos, com nível de significância p OBJECTIVES: The purpose of this study was to investigate what effect the intake of sardines, rich in omega-3 series polyunsaturated fatty acids, has on the composition of breastmilk. METHODS: This was a prospective study of 31 nursing mothers under observation at the Hospital Guilherme Álvaro. Each was given 2 kg of fresh sardines twice with a 15-day interval. Milk was sampled and a 24-hour dietary recall questionnaire was applied on days 0, 15 and 30. Milk was assayed for fatty acid content by gas chromatography. Statistical analysis of the results was performed using nonparametric tests with significance set at p < 0.05. RESULTS: The results demonstrate that the nutritional intake of the nursing mothers was adequate at all three sample points. With regard to the omega-3 series fatty acid content of the breastmilk, it was observed that regular consumption and shorter intervals between intake and milk collection resulted in higher concentrations of docosapentaenoic acid and docosahexaenoic acid at 15 and 30 days into the study. Fatty acids from the omega-3 and omega-6 series exhibited a significant correlation, r² was 0.58 and 0.59 at 15 and 30 days, respectively. CONCLUSION: These results suggest that incorporating fish into the diets of nursing mother during lactation, in the form of 100 g of

  8. Pacific sardine larvae distribution off California

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Marine Sanctuary Program (NMSP) updates and revises the management plans for each of its 13 sanctuaries. This process, which is open to the public,...

  9. Avaliação da utilização de embalagem em atmosfera modificada sobre a conservação de sardinhas (Sardinella brasiliensis Evaluation of the use of modied atmosphere packaging in sardine (Sardinella brasiliensis preservation

    Directory of Open Access Journals (Sweden)

    Anderson Junger Teodoro

    2007-03-01

    Full Text Available A vida útil dos alimentos perecíveis conservados em atmosfera normal é limitada principalmente pelo efeito do oxigênio atmosférico e o crescimento de microorganismos aeróbios produtores de alterações, que promovem mudanças de odor, sabor, cor e textura, conduzindo à perda da qualidade. A modificação da atmosfera prolonga significativamente a vida útil dos alimentos, quando comparados à refrigeração, podendo chegar a um aumento de três a quatro vezes. O trabalho teve como objetivo avaliar o efeito da embalagem de sardinhas (Sardinella brasiliensis em atmosfera modificada. As amostras foram divididas em 8 lotes, embaladas com aproximadamente 1,5 L de ar (100%, CO2 (100%, 50/50 CO2/O2 e vácuo e termo-seladas, sendo armazenadas por um período de 22 dias. A cada 2 dias as amostras foram avaliadas quanto ao pH, bases voláteis totais (BVT e contagens totais de microrganismos heterotróficos aeróbios mesófilos. Os valores de pH aumentaram durante o tempo de estocagem, com exceção das amostras armazenadas em 100 e 50% CO2. A evolução de BVT foi compatível com outros parâmetros, como pH e contagem de bactérias totais, sendo sua evolução mais lenta nas amostras em que ocorreu o uso de atmosfera modificada. Os microrganismos mesófilos, nas atmosferas enriquecidas com CO2, apresentaram fases de latência e tempos de duplicação maiores, quando comparados com os das amostras em que não foi aplicado o método. Sob o ponto de vista microbiológico, as embalagens enriquecidas com CO2 demonstraram ser o melhor método de conservação, como também os resultados de BVT se encontraram dentro dos limites de 30 mg.100 g -1 de acordo com o estabelecido pela RIISPOA8. Recomenda-se o uso da atmosfera de 100% de CO2 como forma de conservação da sardinha por apresentar melhores parâmetros de vida útil.The objective of the work was to evaluate the effect of sardines (Sardinella brasiliensis packaged in a modified atmosphere. The

  10. Abundance and distribution of avian and marine mammal predators ...

    African Journals Online (AJOL)

    The principal predators associated with this activity were common dolphins Delphinus capensis and Cape gannets Morus capensis, and their nearshore distribution was associated with sardine and East Coast round herring E. teres. Few clupeoids were encountered along the KwaZulu-Natal continental shelf, although ...

  11. Variable spatial structure of schooling pelagic fish off Namibia ...

    African Journals Online (AJOL)

    Schools of horse mackerel Trachurus trachurus capensis, sardine Sardinops sagax and round herring Etrumeus whiteheadi were surveyed by conventional echo-integration along systematic transects. The transects were surveyed twice, in opposite directions. Clupeoid biomass was concentrated in a few dense ...

  12. Increasingly, holistic ecosystem approaches are being developed ...

    African Journals Online (AJOL)

    were investigated by means of a posteori tests. Cumulative prey ... mum 12 h) or frozen for later processing ashore. Gonads were ..... (e.g. sardine, round herring), or prefer deeper offshore waters (e.g. .... Cape hake (shallow-water). Merluccius ...

  13. By-catch composition of the Patagonian scallop fishery: the fishes Composición de la captura incidental en la pesquería de vieira patagónica: los peces

    Directory of Open Access Journals (Sweden)

    Laura Schejter

    2012-11-01

    Full Text Available An inventory of 24 fish species incidentally caught by the Patagonian scallop fleet in the SW Atlantic Ocean is provided for the first time. The most frequent species were Psammobatis spp. (81.4%, Bathyraja brachyurops (75.1%, B. macloviana (73.5%, Patagonotothen ramsayi (66.1%, Merluccius hubbsi (53.7% and B. albomaculata (50.3%. Many of the recorded chondrichthyes are considered vulnerable or endangered species. The number of taxa (fishes + invertebrates that conforms the by-catch of the fishery was increased and updated to nearly 200 species.Este estudio presenta por primera vez un inventario con 24 especies de peces registradas en la captura incidental de la pesca de la vieira patagónica en el Océano Atlántico sudoccidental por la flota pesquera comercial. Las especies más frecuentes fueron Psammobatis spp. (81,4%, Bathyraja brachyurops (75,1%, B. macloviana (73,5%, Patagonotothen ramsayi (66,1%, Merluccius hubbsi (53,7% y B. albomaculata (50,3%. Muchos de los condrictios registrados se encuentran actualmente considerados como especies vulnerables o en peligro. El número de taxa (peces + invertebrados que conforman la captura incidental de esta pesquería se incrementó y actualizó con estos resultados a aproximadamente 200 especies.

  14. A study of the life history of brazilian sardine, Sardinella brasiliensis.: III. development of sardine larvae

    Directory of Open Access Journals (Sweden)

    Yasunobu Matsuura

    1975-01-01

    Full Text Available Larvae and juveniles of S. brasiliensis (Steindachner, 1879, ranging from 6.4 mm to 35.5 mm, were identified from plankton samples taken in waters off the southern Brazilian coast from 1969 through 1971. Changes in the pattern of pigmentation, body proportions and formation of fin rays are described. During transformation stage a considerable advancement of the dorsal and anal fins was observed. Changes in body proportions are pronounced at the size of 19 mm. Complete ossification of all fin rays is attained at the size of 20 mm, but ossification of the ventral scutes is delayed and completed only at the size of 30 mm. Ossification of the vertebral column was completed at the size of about 16 mm.O presente trabalho é parte do projeto SOL e tem por objetivo descrever a morfologia das larvas da sardinha verdadeira, Sardinella brasiliensis (=S. aurita, com o intuito de determinar um padrão que possibilite a identificação das mesmas. São feitas considerações sobre a morfologia das larvas de outros clupeídeos existentes na região e ressaltadas as diferenças com relação as larvas de S. brasiliensis. Os resultados, posteriormente, serão usados como base para o estudo quantitativo da abundância de larvas de sardinha. O material foi coletado na costa sul do Brasil de 1969 a 1971, com uma rede de plâncton do tipo cônico-cilíndrico. Durante o desenvolvimento das larvas, foi observado um considerável deslocamento das bases das nadadeiras dorsal e anal, para uma posição mais anterior. Com o tamanho de 19 mm (comprimento padrão, ocorre uma mudança geral, considerável, nas proporções corporais. A ossificação de todos os raios das nadadeiras completa-se quando a larva atinge 20 mm, mas a ossificação dos escudos ventrais só se completa quando ela atinge 30 mm de comprimento. A ossificação das vertebras completa-se a 16 mm de comprimento. O tamanho de 19 mm foi considerado como o fim do estagio larval, e, apos este comprimento, consideramos a larva no estagio prejuvenil (=transformal.

  15. Experimental study of heat transfer and pressures drops for cans with spiral herring-bone fins; Etude experimentale du transfert de chaleur et des pertes de charges des gaines a ailettes helicoidales en chevron

    Energy Technology Data Exchange (ETDEWEB)

    Pelce, J; Francois, S; Houseaux, O; Pierre, B [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-07-01

    Cans fitted with herring-bone fins are used for cooling uranium in certain nuclear reactor. By herring-bone is meant a staggered arrangement of the fins which have a plane of symmetry parallel to the general direction of liquid flow. The main geometrical parameter are then: the number of fins, the number of herring-bones, the angle of inclination of the fins with respect to the can axis, the dimensions of the fins, the can diameter and the channel diameter. The research is essentially experimental. The test are of three types: full size tests, in conditions approaching those in the reactor (constant flux, CO{sub 2} under pressure); full size tests but with a constant wall temperature, much easier to set up, and intended to distinguish rapidly between the merits of the various types of can; large-scale tests with air at atmospheric pressure for studying the phenomena in more detail. For each can tried out there is a corresponding pressure drop coefficient, a mean thermal exchange coefficient Mo-bar and a minimum exchange coefficient Mo{sub min} and Mo-bar are related by the expression Mo{sub min} = Mo-bar * f{sub c} * f, where f{sub c} and f are respectively circumferential and longitudinal singularity factor determined from a statistical study of all the temperatures measured for each can. The results are presented in about thirty tables and figures the most noteworthy results being summarized in the conclusion. (authors) [French] Les gaines a ailettes en chevron sont utilisees pour le refroidissement de l'uranium dans certains reacteurs nucleaires. Par chevron, on entend une disposition alternee des ailettes ayant un plan de symetrie parallele a la direction generale de l'ecoulement fluide. Les principaux parametres geometriques sont alors: le nombre des ailettes, le nombre de chevrons, l'angle d'inclinaison des ailettes par rapport a l'axe de la gaine, les dimensions des ailettes, le diametre de la gaine et le diametre du canal. L'etude est essentiellement

  16. The Patagonian scallop fishing grounds in shelf break frontal areas: the non assessed benthic fraction Bancos de pesca de vieira patagónica en áreas del frente de talud: fracción bentónica no evaluada

    Directory of Open Access Journals (Sweden)

    María de los Ángeles Sánchez

    2011-01-01

    Full Text Available In this study, a Picard dredge was used to sample the benthic community in shelf break frontal areas off Argentina in order to detect species that could be sensitive to fishing activities but are not usually caught during the annual monitoring of Patagonian scallop and associated fauna. The present results show at least 62 taxa not reported previously as components of the benthos in the scallop grounds that are potentially subjected to trawling disturbance.En este trabajo se utilizó una rastra Picard para muestrear la comunidad bentónica en áreas del frente de talud, Argentina, para detectar especies que serían sensibles a la actividad pesquera pero no son capturadas usualmente durante los monitoreos anuales de vieira patagónica y fauna asociada. Los resultados muestran que al menos 62 taxa, no reportados previamente como componentes del bentos en los bancos de vieira, están potencialmente sujetos a perturbaciones por el arrastre.

  17. Eat Seafood Twice a Week: 10 Tips to Help You Eat More Seafood

    Science.gov (United States)

    ... Pacific mackerel, herring on a salad, or oven-baked pollock. 7 put it on a salad or in a sandwich Top a salad with grilled scallops, shrimp, or crab in place of steak or chicken. Use canned tuna or salmon for sandwiches in place of deli meats, which ...

  18. Multicollinearity is a red herring in the search for moderator variables: A guide to interpreting moderated multiple regression models and a critique of Iacobucci, Schneider, Popovich, and Bakamitsos (2016).

    Science.gov (United States)

    McClelland, Gary H; Irwin, Julie R; Disatnik, David; Sivan, Liron

    2017-02-01

    Multicollinearity is irrelevant to the search for moderator variables, contrary to the implications of Iacobucci, Schneider, Popovich, and Bakamitsos (Behavior Research Methods, 2016, this issue). Multicollinearity is like the red herring in a mystery novel that distracts the statistical detective from the pursuit of a true moderator relationship. We show multicollinearity is completely irrelevant for tests of moderator variables. Furthermore, readers of Iacobucci et al. might be confused by a number of their errors. We note those errors, but more positively, we describe a variety of methods researchers might use to test and interpret their moderated multiple regression models, including two-stage testing, mean-centering, spotlighting, orthogonalizing, and floodlighting without regard to putative issues of multicollinearity. We cite a number of recent studies in the psychological literature in which the researchers used these methods appropriately to test, to interpret, and to report their moderated multiple regression models. We conclude with a set of recommendations for the analysis and reporting of moderated multiple regression that should help researchers better understand their models and facilitate generalizations across studies.

  19. Distribution and movement of scalloped hammerhead Sphryna ...

    African Journals Online (AJOL)

    In the Western Cape, Southern Cape and Eastern Cape, few sharks were tagged during the autumn/winter months, whereas in KwaZulu-Natal and Transkei sharks were tagged throughout the year. Large-scale directional movements observed may have been migrations in response to seasonal sea surface temperature ...

  20. Heat exchange and pressure drop of herring-bone fin surfaces. Experimental cell results at constant wall temperature; Echange de chaleur et perte de charge de surfaces a ailettes en chevrons. Resultats experimentaux en cellule a temperature de paroi constante

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1968-07-01

    The increase in the specific power of nuclear reactors of the gas-graphite type has necessitated the use of high performance exchange surfaces for canning the fuel (natural uranium). For this, experiments were carried out on cans fitted with herring-bone fins, at constant wall temperature; a flow of water at 100 deg. C passes inside the can which is cooled externally by a flow of CO{sub 2} at 15 bars pressure. This experimental set-up makes it possible to compare the aero-thermal performances of the different cans with an accuracy of 5 per cent. This report presents the results obtained in the form of a friction coefficient f{sub 0} and mean Margoulis number m{sub 0} as a function of the Reynolds number Re{sub 0}, this latter varying from 3 x 10{sup 5} to 9 x 10{sup 5}. (authors) [French] L'augmentation de la puissance specifique des reacteurs nucleaires de la filiere graphite-gaz a necessite l'utilisation de surfaces d'echange a hautes performances pour gainer le combustible (uranium naturel). Dans cette optique, des gaines munies d'ailettes disposees en chevron ont ete experimentees a temperature de paroi constante: un courant d'eau a 100 deg. C circule a l'interieur de la gaine qui est refroidie exterieurement par un ecoulement de CO{sub 2} sous une pression de 15 bars. Cette methode experimentale permet de situer les performances aerothermiques des gaines les unes par rapport aux autres a 5 pour cent pres. Ce rapport presente les resultats obtenus sous la forme d'un coefficient de frottement f{sub 0} et d'un nombre de Margoulis moyen m{sub 0} en fonction du nombre de Reynolds Re{sub 0}, ce dernier pouvant varier de 3. 10{sup 5} a 9. 10{sup 5}. (auteurs)

  1. Reproductive and larval cycle of the scallop Argopecten purpuratus (Ostreoida: Pectinidae, during El Niño-La Niña events and normal weather conditions in Antofagasta, Chile

    Directory of Open Access Journals (Sweden)

    Miguel Avendaño

    2008-03-01

    Full Text Available Seasonality, amplitude, and magnitude of spawning events were determined for Argopecten purpuratus in the La Rinconada marine reserve, Antofagasta, Chile, between December 1995 and January 2004. During the same period, samples of scallop larvae were obtained in vertical plankton hauls recovered within this reserve in an area routinely exposed to circular, gyre-like currents which helped retain the larvae within the bay. The reproduction of this population in normal or cool (e.g. "La Niña", 1998-2000 years occurred throughout the year, with a more active period between September and April, declining in June and August; this contrasted with the warmer "El Niño" oceanographic period of 1997-98 in which reproductive activity was more intense and prolonged throughout the entire year. The reproductive events in this population were mostly synchronous, although one asynchronous period occurred each year following the more intense March to May spawnings. This reproductive activity generated a continuous presence of larvae in the area in which no strict relation could be found between the intensities of spawning and numbers of larvae in the water. Larval presence was, however, generally correlated with active spawning periods. Important increases in larval numbers recorded at the end of 1999 and the beginning of 2003 were correlated with census data showing a higher percentage presence of broodstock over 90 mm in shell length during these years. An adequate stock of this size class is needed for a successful seed capture program in the reserve (for mass culture. Rev. Biol. Trop. 56 (1: 121-132. Epub 2008 March 31.Entre 1995 y 2004 se determinó, con el índice gonadosomático, el ciclo reproductivo de Argopecten purpuratus en La Rinconada, Antofagasta, Chile. Paralelamente se realizaron muestreos larvales mediante arrastres verticales de plancton. La reproducción, en años normales y fríos (La Niña, 1998-2000, ocurre todo el año, con un período m

  2. Influence of environmental factors on the growth of the juvenile, maturing juvenile, and adult tropical scallop, Euvola ziczac (Pteroida: Pectinidae, in suspended culture conditions Influencia de los factores ambientales en el crecimiento de juveniles, juveniles con capacidad reproductiva y adultos de la vieira tropical Euvola ziczac (Pteroida: Pectinidae en condiciones de cultivo suspendido

    Directory of Open Access Journals (Sweden)

    César Lodeiros

    2012-03-01

    Full Text Available We carried out growth trials on three size groups of the scallop, Euvola ziczac, during two short-term (34-36 days periods at 8, 21, and 34 m depth in Cariaco Gulf, Venezuela, in order to evaluate the effect of environmental factors on growth. Growth was greater in juveniles (initial size: 20-22 mm shell length than in maturing juveniles (30-40 mm and adults (70-75 mm. Somatic tissue growth was more than two-fold greater in juveniles than in maturing individuals. Adult scallops showed losses of tissue mass. The first period (December-January covered the transition from a stratified water column to upwelling conditions, with initial high temperatures and low phytoplankton biomass followed by lower temperatures and greater phytoplankton biomass. The second period (February-March consisted of upwelling, characterized by low temperatures and high phytoplankton production. The greater growth of juvenile and maturing scallops during the second period, particularly at 8 m depth, was associated with the greater availability of phytoplankton, related to coastal upwelling. The first development of gonads in maturing scallops only occurred in the second period, associated with food availability, and the degree of development was correlated with the depth. Our growth trials, which were too short to permit the negative impact of the development of fouling, showed that the growth of E. ziczac was enhanced in the presence of abundant phytoplankton biomass.Se efectuaron ensayos de crecimiento en tres grupos de tallas del pectínido Euvola ziczac, durante dos periodos de corto plazo (34-36 días a 8, 21 y 34 m de profundidad, en el golfo de Cariaco, Venezuela, para evaluar el efecto de factores ambientales en el crecimiento. El crecimiento fue mayor en juveniles (talla inicial 20-22 mm en longitud de concha, que en individuos madurando (30-40 mm y adultos (70-75 mm. El crecimiento del tejido somático fue más del doble en los juveniles que en los organismos en

  3. Boom-to-bust. The scallop (Argopecten purpuratus fishery in the Pisco-Paracas region, southern Peruvian coast Auge y crisis: la pesquería de la concha de abanico (Argopecten purpuratus en la región Pisco-Paracas, costa sur del Perú

    Directory of Open Access Journals (Sweden)

    Ricardo M. González Hunt

    2010-12-01

    Full Text Available This paper examines scallop (Argopecten purpuratus booms experienced in the Pisco-Paracas Region of southern Peru, triggered by the 1982-1983 and the 1997-1998 mega-El Niño Southern Oscillation (ENSO events.The quiet fishing ports have been transformed by these booms, which have attracted outside stakeholders transforming the local society. Government institutions in their role as resource managers and environmental stewards have attempted to control access to a region that until recently contained the only marine protected area of Peru.This situation has led to rapid growth in the scallop industry, the overexploitation and depletion of the shellfish, creating a sustainability crisis. Furthermore, this paper examines contradictions and relationships across local, regional, national, and international scales.Este trabajo examina los ciclos de expansión (boom de la explotación de la concha de abanico (Argopecten purpuratus observados en la región Pisco-Paracas del sur del Perú, resultantes de los fenómenos El Niño de 1982-1983 y 1997-1998.Los apacibles puertos de pesca han sido transformados por estos booms productivos que han atraído actores externos y han generado un impacto en la sociedad local. Las instituciones gubernamentales, en su papel de administradores de recursos y protectores del medio ambiente, han tratado de controlar el acceso a una región que hasta hace poco contenía la única área marina protegida del Perú.Esta situación ha producido un rápido crecimiento de la industria de la concha de abanico, su sobreexplotación y el agotamiento de dicho recurso, y ha producido una crisis de sostenibilidad. Asimismo, este trabajo examina las contradicciones y las relaciones entre las escalas local, regional, nacional e internacional.

  4. Clasificación acústica de anchoveta (Engraulis ringens y sardina común (Strangomera bentincki mediante máquinas de vectores soporte en la zona centro-sur de Chile: efecto de la calibración de los parámetros en la matriz de confusión Acoustic classification of anchovy (Engraulis ringens and sardine (Strangomera bentincki using support vector machines in central-southern Chile: effect of parameter calibration on the confusion matrix

    Directory of Open Access Journals (Sweden)

    Hugo Robotham

    2012-03-01

    Full Text Available Se clasificó la anchoveta (Engraulis ringens y sardina común (Strangomera bentincki detectadas mediante equipos acústicos en la zona centro-sur de Chile, mediante el método de Máquinas de Vectores Soporte (SVM. Para esto se utilizaron descriptores de cardúmenes extraídos desde ecogramas, que fueron clasificados como morfológicos, batimétricos, energéticos y posicional espacial. Para lograr clasificaciones precisas mediante la utilización de esta metodología, fue necesario optimizar parámetros correspondientes al Kernel-Gaussiano, γ y de penalización del modelo C, mediante el análisis del efecto de la calibración sobre las matrices de confusión resultantes de la clasificación de las especies analizadas. El método SVM ajustó correctamente el 95,3% de los cardúmenes de anchoveta y sardina común. Los parámetros óptimos del Kernel-Gaussiano γ y de penalización C obtenidos mediante la metodología propuesta fueron γ = 450 y C = 0,95, respectivamente. Los parámetros mencionados incidieron de manera importante en la matriz de confusión y los porcentajes de clasificación final, por lo que se sugiere establecer, en aplicaciones futuras de este método, un protocolo experimental de calibración. La sardina común fue la especie con menor error de clasificación en el conjunto de las matrices de confusión. El descriptor correspondiente a profundidad del fondo fue el más sensible al SVM, la segunda variable en importancia es el descriptor distancia a la costa.The support vector machines (SVM method was used to classify the anchovy (Engraulis ringens and common sardine (Strangomera bentincki species detected in south-central Chile by means of acoustic equipment. For this, descriptors of fish schools (morphology, bathymetry, energy, spatial position extracted from ecograms were used. In order to obtain precise classifications using this methodology, it was necessary to optimize the parameters Gaussian-Kernel γ and penalty term

  5. Simulation model of the scallop (Argopecten purpuratus farming in northern Chile: some applications in the decision making process Modelo de simulación para el cultivo del ostión (Argopecten purpuratus en el norte de Chile: aplicaciones para la toma de decisiones

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    Renato Molina

    2012-09-01

    Full Text Available Aquaculture farming is a complex system integrating several disciplines, including biology, engineering and economics, all which need to be correctly intertwined to have a profitable and environmentally sustainable activity. During the past recent years, scallop (Argopectenpurpuratus farmers in northern Chile have come to comprehend the hard way that aquaculture producers operate in a complex and dynamic environment where natural and economic factors are in constant change. Thus, to keep a profitable and competitive business in today's world, aquaculture farm managers are in need of relatively easy to use tools for efficient and timely decision making. Harvest size and time, mortality and growth rates, stocking rates, costs and market prices are important variables and parameters to monitor, where decisions with respect to their levels or values have to be made. In this context, non-linear and dynamic quantitative bioeconomic models should become valuable tools, for periodic decision making in the aquaculture business. This paper shows how to emulate Chilean scallop farming using a simulation model that mimics some of the industry's features. The model presented here focuses on a scallop aquaculture center that uses the common technology approach of pearl net and lanterns of the northern region of Chile, and analyses the farming strategies based on harvesting size. Also, these strategies were subject to variations in the parameters in order to identify patterns and asses the sensibility of the model to input values.La acuicultura es un sistema complejo que integra varias disciplinas, incluyendo la biología, ingeniería y economía, las cuales deben ser correctamente entrelazadas para lograr una actividad rentable y ambientalmente sostenible. Durante los últimos anos, los cultivadores del ostión del norte (Argopecten purpuratus en Chile han comprendido de la peor manera, que las actividades de acuicultura operan en un entorno complejo y din

  6. Asociación entre la tasa de autofecundación y la frecuencia de larvas malformadas en poblaciones cultivadas del ostión del norte Argopecten purpuratus (Lamarck, 1819 Association between self-fertilization rates and the frequency of malformed larvae in farmed populations of the northern scallop Argopecten purpuratus (Lamarck, 1819

    Directory of Open Access Journals (Sweden)

    Christopher Concha

    2011-07-01

    Full Text Available El incremento de la frecuencia de malformaciones y la reducción de la viabilidad y fecundidad suelen ser las primeras manifestaciones de la depresión por consanguinidad en animales. El ostión del norte, Argopectenpurpuratus (Lamarck, 1819, es una especie hermnafrodita funcional con autofecundación parcial y durante la reproducción artificial puede presentar altos grados de autofecundación. En este trabajo se analizó la asociación de la tasa de autofecundación con la frecuencia de larvas malformadas y la supervivencia larval. Se desovaron adultos maduros y se recogieron separadamente los gametos femeninos del 5° pulso de liberación en adelante y los masculinos. Los ovocitos fueron fecundados con espermatozoides de otro individuo, formando familias de hermanos completos. La tasa de autofecundación se verificó por la proporción de ovocitos que entra en división en una muestra de ellos sin fecundar. La tasa de autofecundación varió entre familias de 0 a 100%, con distribución de frecuencias normal. La proporción de larvas malformadas se distribuyó al azar entre las familias analizadas, pero se correlacionó negativamente, en forma moderada pero significativa, con la tasa de autofecundación y la temperatura media del cultivo. Los datos sugieren que la autofecundación en las familias de ostiones puede favorecer una mayor homeostasis del desarrollo larval.Increased frequencies of malformations and the reduction of viability and fecundity are some of the first manifestations of inbreeding depression in animals. The northern scallop, Argopecten purpuratus (Lamarck, 1819, is a functional hermaphrodite species with partial self-fertilization. During artificial reproduction, this species may present high degrees of self-fertilization. In this work, the association between the selfing rate and the frequency of larval malformations and survival were analyzed. Mature adults were spawned, and female and male gametes were collected

  7. Anchovy Engraulis capensis and sardine Sardinops sagax currently ...

    African Journals Online (AJOL)

    spamer

    Recruitment is thought to be determined largely by ... for forecasting recruitment strength. ..... In March and April, sustained upwelling wind events ... 2 Assumes average current velocities of 10–15 cm.s–1 in a west-north-westerly direction ...

  8. The southern African sardine Sardinops sagax supports major purse ...

    African Journals Online (AJOL)

    spamer

    be caused by environmental factors such as light level and currents, as well as by ... 2 Marine & Coastal Management, Department of Environmental Affairs and Tourism, Private Bag X2, Rogge Bay 8012, Cape Town,. South Africa. 3 Institut de ..... An impression, but one that was difficult to quantify, was that the detection rate ...

  9. Composição em aminoácidos de silagens químicas, biológicas e enzimáticas preparadas com resíduos de sardinha Determination of amino-acid composition of silages prepared from sardine residues

    Directory of Open Access Journals (Sweden)

    Doris Floridalma MORALES-ULLOA

    1997-12-01

    Full Text Available Determinou-se a composição em aminoácidos de silagens químicas, biológicas e enzimáticas elaboradas com resíduos de sardinha. Entre os aminoácidos essenciais a leucina apresentou valores mais altos para todas as silagens, a saber, em g/100g de proteína, 8,31 (química; 8,33 (protease 1 semana; 8,42 (pepsina, e 8,06 (inóculo L. plantarum + melaço 2 semanas, seguida pela lisina 6,46; 6,50 6,45, e 9,01; a fenilalanina com 5,32; 5,35 e 5,25 e 5,18. Destaque especial para o aumento na concentração de valina no decorrer do processo de ensilagem passando de 4,80 g/100g de proteína na matéria-prima para 7,67 na silagem química (3 semanas; 6,26 na silagem com meio inóculo de L.plantarum + melaço (48 horas; 6,27 na silagem protease (1 semana e 6,02 na silagem pepsina (2 semanas. A maior concentração de aminoácidos encontrados foi para o ácido glutâmico, que apresentou teor inicial de 15,20g/100g de proteína e posteriormente 14,02 na silagem química após 1 semana; 14,89 na silagem enzimática com protease (1 semana e 17,09 na silagem biológica com meio inóculo L.plantarum + melaço após 48 horas.The composition and amino-acid concentration of chemical, biological and enzymatic silages prepared from sardine residues, were determined. The essential amino-acid, leucine showed the highest values in all silages (8.31; 8,33 8.42; and 8.06 g/100g protein, followed by lysine (6.46, 6.50, 6.45 and 9,.01 g/100 protein, for chemical silage, protease silage after one week pepsin silage in the L. plantarum after 2 weeks respectively. Phenylalanine showed a value of 5.32g/100g protein in the chemical silages after one week, 5.35g/100g in the protease silage after one week, 5.25g/100g in the pepsin silage after two weeks and 5.18g/100g in the silage inoculated with L. plantarum plus its medium, also after two weeks. The increase in the valine during the silage processing deserves special mention, increasing from 4.80/100g protein in the raw

  10. Validade comercial de sardinhas inteiras e refrigeradas avaliada por análises físico-químicas, bacteriológicas e sensorial Shelf life of chilled and whole sardines evaluated by physical-chemical, bacteriological and sensory

    Directory of Open Access Journals (Sweden)

    Sabrina da Costa Silva Andrade

    2012-10-01

    Full Text Available A validade comercial de sardinhas das espécies Sardinella brasiliensis e Cetengraulis edentulus mantidas sob refrigeração em gelo (0+2°C foi determinada por parâmetros analíticos físico-químicos, bacteriológicos e sensorial. Nas duas amostras, os teores de Bases Voláteis Totais (BVT e Trimetilamina (TMA atingiram o limite máximo recomendado na legislação (30mg N100g-1 para BVT e 4mg N100g-1 para TMA após 14 e 8 dias de estocagem, respectivamente. O conteúdo de histamina, putrescina e cadaverina se manteve em níveis inferiores a 2.0µg g-1 nas duas amostras durante o período de estocagem. A produção de hipoxantina variou de 0,65 a 2,62µmol g-1 nas amostras de S. brasiliensis e de 1,40 a 2,09µmol g-1 nas amostras de C. edentulus. A contagem inicial de Enterobacteriaceae foi de 3,81log UFC g-1 e 3,82log UFC g-1 atingindo, ao final de 18 dias de estocagem, 6,57log UFC g-1 e 6,87log UFC g-1, nas amostras de S. brasiliensis e C. edentulus, respectivamente. Para as contagens de bactérias heterotróficas aeróbias mesófilas e psicrotróficas, o limite de 7log UFC g-1 preconizado na legislação internacional foi alcançado após o 12° e 8° dias de estocagem nas amostras de S. brasiliensis e após o 12° e 6° dias de estocagem nas amostras de C. edentulus, respectivamente. O método de índice de qualidade sugeriu, para as amostras de S. brasiliensis, um limite de consumo aceitável inferior a 11 e, para as amostras de C. edentulus, um limite de aceitabilidade inferior a 14. Foi proposta a validade comercial de dez dias para a S. brasiliensis e nove dias para o C. edentulus.Theshelf life of sardines of Sardinella brasiliensis and Cetengraulis edentulus species kept on ice at +2°Cwas determined by physical-chemical, bacteriological and sensory parameters. In both samples, the levels of Total Volatile Bases (TVB and Trimethylamine (TMA reached the limits recommended by law (30mg N 100g-1 for TVB and 4mgN100g-1 for TMA after 14

  11. Theoretical and experimental study of heat transfers and pressure drops along surfaces fitted with herring-bone fins: correlation between geometric and aero thermal parameters; Etudes theorique et experimentale du transfert de chaleur et des pertes de charge de surfaces munies d'ailettes disposees en chevron - correlation entre parametres geometriques et aerothermiques

    Energy Technology Data Exchange (ETDEWEB)

    Pelce, J; Malherbe, J; Pierre, B [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1965-07-01

    Principal results are given of experimental research which has been carried out on the flow of a fluid along a surface fitted with herringbone fins. Aero-thermal tests have been effected on a large number of these surfaces whose geometrical parameters have been made to vary systematically. In particular, work on a large scale model has made it possible to analyse the mechanisms of heat transfer and of pressure drops. On this basis a theoretical study has led to the establishment of a correlation between the geometric configuration and the aero-thermal performances of these surfaces. Experimental results are in good agreement with the theoretical relationships. An expression has thus been derived applicable to this type of herring-boned surface in a wide zone. (authors) [French] L'ecoulement d'un fluide au voisinage d'une surface munie d'ailettes disposees en chevron a fait l'objet de recherches experimentales dont on a rappele les principaux resultats. Des essais aerothermiques ont ete effectues sur un grand nombre de ces surfaces dont a fait varier les parametres geometriques de facon systematique. En particulier, des etudes sur une maquette a grande echelle ont permis d'analyser les mecanismes de transfert de chaleur et de perte de charge. Sur ces bases, une etude theorique a conduit a des correlations entre la geometrie et les performances aerothermiques de ces surfaces. Les resultats experimentaux sont en bon accord avec les relations theoriques. On possede ainsi une formulation pour ce type de surface ailettee valable dans un domaine etendu. (auteurs)

  12. The biochemical, textural and sensory properties of king scallop ...

    African Journals Online (AJOL)

    USER

    2010-07-12

    Jul 12, 2010 ... fluids and myofibrillar protein denaturation (Ca2+-ATPase activities in actomyosin extracts). ... much prized as food and its adductor muscle is offered to ..... and/or mechanical disruption of mitochondrial membranes.

  13. Total protein and lipid contents of canned fish on the Serbian market

    OpenAIRE

    Marković Goran; Mladenović Jelena; Cvijović Milica; Miljković Jelena

    2015-01-01

    Total protein and lipid contents were analysed in 5 samples of canned fish (sardines, Atlantic mackerel fillets, tuna in olive oil, smoked Baltic sprat and herring fillets) available on the Serbian market. Standard methods for the determination of protein (Kjeldahl method) and lipid (Soxhlet method) contents were used on drained samples. The protein content was 21.31% on average, with a range of 18.59% - 24.17%. Total lipids showed considerably large variations (5.49% - 35.20%), and averaged ...

  14. Diet variability of forage fishes in the Northern California Current System

    Science.gov (United States)

    Hill, Andrew D.; Daly, Elizabeth A.; Brodeur, Richard D.

    2015-06-01

    As fisheries management shifts to an ecosystem-based approach, understanding energy pathways and trophic relationships in the Northern California Current (NCC) will become increasingly important for predictive modeling and understanding ecosystem response to changing ocean conditions. In the NCC, pelagic forage fishes are a critical link between seasonal and interannual variation in primary production and upper trophic groups. We compared diets among dominant forage fish (sardines, anchovies, herring, and smelts) in the NCC collected in May and June of 2011 and June 2012, and found high diet variability between and within species on seasonal and annual time scales, and also on decadal scales when compared to results of past studies conducted in the early 2000s. Copepoda were a large proportion by weight of several forage fish diets in 2011 and 2012, which differed from a preponderance of Euphausiidae found in previous studies, even though all years exhibited cool ocean conditions. We also examined diet overlap among these species and with co-occurring subyearling Chinook salmon and found that surf smelt diets overlapped more with subyearling Chinook diets than any other forage fish. Herring and sardine diets overlapped the most with each other in our interdecadal comparisons and some prey items were common to all forage fish diets. Forage fish that show plasticity in diet may be more adapted to ocean conditions of low productivity or anomalous prey fields. These findings highlight the variable and not well-understood connections between ocean conditions and energy pathways within the NCC.

  15. Teaching to the Test: A Very Large Red Herring

    Science.gov (United States)

    Phelps, Richard P.

    2016-01-01

    Elevating teaching-to-the-test to dogma, from the beginning with the distortion of J.J. Cannell's 1980s "Lake Wobegon" findings, has served to divert attention from scandals that should have threatened US educators' almost complete control of their own evaluation. Had the test cheating scandal Dr. Cannell uncovered been portrayed…

  16. Of conflicts, conspiracies, red herrings, and black swans.

    Science.gov (United States)

    Wactawski-Wende, J; Anderson, G L

    2015-06-01

    The impact of the findings from the Women's Health Initiative trial of estrogen plus progestin cannot be attributed to any real or imagined conflicts of interest between government, researchers, and journals. Rather, the findings overturned decades of dogma in part promoted by the pharmaceutical industry, and the reaction to these unexpected findings was in direct proportion to their importance in reversing a misguided practice of prescribing the drug for chronic disease prevention. The findings have been widely accepted, as shown by the sustained subsequent reduction in prescriptions. However, conflicts of interest may influence a minority unwilling to accept the findings. The decrease in the use of a drug with an adverse risk profile for prevention of chronic disease is a public good.

  17. The medieval Herring Fishery in the Western Baltic

    DEFF Research Database (Denmark)

    Jahnke, Carsten

    2008-01-01

    Artiklen handler om sammenhang mellen den skånske sildefiskerier og den central Europæiske marked. Hvorfor blev en østersøfisk hverdagsspise i hele Europa? Og hvilke konsekvenser har markedsudviklinger haft på fiskeriet? Samtidig beskriver artiklen Skåne Markedets opkomst og udvikling....

  18. Teaching to the test: A very large red herring

    Directory of Open Access Journals (Sweden)

    Richard P. Phelps

    2016-05-01

    Full Text Available Elevating teaching-to-the-test to dogma, from the beginning with the distortion of Dr. Cannell’s findings, has served to divert attention from scandals that should have threatened US educators’ almost complete control of their own evaluation.[10] Had the scandal Dr. Cannell uncovered been portrayed honestly to the public—educators cheat on tests administered internally with lax security—the obvious solution would have been to externally manage all assessments (Oliphant, 2011. Recent test cheating scandals in Atlanta, Washington, DC, and elsewhere once again drew attention to a serious problem. But, instead of blaming lax security and internally managed test administration, most educators blamed the stakes and alleged undue pressure that ensues (Phelps 2011a. Their recommendation, as usual: drop the stakes and reduce the amount of testing. Never mind the ironies: they want oversight lifted so they may operate with none, and they admit that they cannot be trusted to administer tests to our children properly, but we should trust them to educate our children properly if we leave them alone. Perhaps the most profound factoids revealed by the more recent scandals were, first, that the cheating had continued for ten years in Atlanta before any responsible person attempted to stop it and, even then, it required authorities outside the education industry to report the situation honestly. Second, in both Atlanta and Washington, DC, education industry test security consultants repeatedly declared the systems free of wrongdoing (Phelps 2011b. Meanwhile, thirty years after J. J. Cannell first showed us how lax security leads to corrupted test scores, regardless the stakes, test security remains cavalierly loose. We have teachers administering state tests in their own classrooms to their own students, principals distributing and collecting test forms in their own schools. Security may be high outside the schoolhouse door, but inside, too much is left to chance. And, as it turns out, educators are as human as the rest of us; some of them cheat and not all of them manage to keep test materials secure, even when they aren’t intentionally cheating. - See more at: http://nonpartisaneducation.org/Review/Essays/v12n1.htm

  19. Imitation in European herring fisheries, c. 1550-1860

    DEFF Research Database (Denmark)

    Poulsen, Bo

    2016-01-01

    . This paper explores the consequences of the image of Dutch dominance, as seen through 41 different attempts to build a fleet and run fishing operations similar to the Dutch. Most of them were short lived, and some never made it to the fishing grounds before going bankrupt. When reviewed one by one, they all...

  20. Catch, effort and sampling strategies in the highly variable sardine fisheries around East Java, Indonesia.

    NARCIS (Netherlands)

    Pet, J.S.; Densen, van W.L.T.; Machiels, M.A.M.; Sukkel, M.; Setyohady, D.; Tumuljadi, A.

    1997-01-01

    Temporal and spatial patterns in the fishery for Sardinella spp. around East Java, Indonesia, were studied in an attempt to develop an efficient catch and effort sampling strategy for this highly variable fishery. The inter-annual and monthly variation in catch, effort and catch per unit of effort

  1. Artificial neural networks to forecast biomass of Pacific sardine and its environment

    DEFF Research Database (Denmark)

    Cisneros Mata, M.A.; Brey, T.; Jarre, Astrid

    1996-01-01

    We tested the forecasting performance of artificial neural networks (ANNs) using several time series of environmental and biotic data pertaining to the California Current (CC) neritic ecosystem. ANNs performed well predicting CC monthly 10-m depth temperature up to nine years in advance, using te...

  2. Mercury in canned fish from local markets in the Czech Republic.

    Science.gov (United States)

    Kral, Tomas; Blahova, Jana; Sedlackova, Lenka; Kalina, Jiri; Svobodova, Zdenka

    2017-06-01

    The aim of this survey was to compare total mercury (THg) in canned fish in terms of species and fishing area. A number of 110 samples of canned fish, which were divided by fish species and fishing area, were analysed. The highest THg content in muscle tissue was found in the escolar. In other fish species, the highest level was detected in tuna. Mercury content in tuna differed significantly from mackerel, sardine, and sprat. Mercury content in herring differed significantly from sprat. The order of the fish according to their THg content corresponds to their status in the aquatic food chain. A significant difference was also found between fish caught from the closed Mediterranean and Black Sea and fish caught in the open oceans. In terms of THg content, the results of this study indicate good quality of various species of canned fish caught in worldwide locations.

  3. Occurrence and spatial distribution of chemical contaminants in edible fish species collected from UK and proximate marine waters.

    Science.gov (United States)

    Fernandes, A R; Mortimer, D; Holmes, M; Rose, M; Zhihua, L; Huang, X; Smith, F; Panton, S; Marshall, L

    2018-05-01

    The occurrence of a range of regulated and emerging organic environmental contaminants was investigated in 182 samples of edible marine fish sampled mainly from UK marine regions, but extending northerly to the coast of Norway and south to the Algarve. These species (sprats, mackerel, turbot, halibut, herring, grey mullet, sea bass, grey mullet, sardines, etc.) are among those considered to be at the highest risk of contamination with regulated contaminants such as polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs, dioxins), and polychlorinated biphenyls (PCBs), but the occurrence of polybrominated diphenylethers (PBDEs) and polybrominated biphenyls (PBBs) was also investigated. Sub-sets of samples (50-75) were also analysed for emerging contaminants: polychlorinated naphthalenes (PCNs), polybrominated and mixed halogenated dibenzo-p-dioxins, dibenzofurans and biphenyls (PBDD/Fs, PXDD/Fs and PXBs) and perfluoroalkyl substances (PFAS). Contaminant occurrence varied with species and location, but all measured contaminants were detected, with sprats, sea bass, sardines, mackerel, and herring showing higher tissue concentrations. The concentrations of the different contaminants in the various samples were mapped utilising the GPS coordinate data of the capture locations to visualise spatial distribution levels. In terms of catch location, fish sampled from the coasts of southern Britain, north-western France and the Irish Sea appeared to contain proportionately higher levels of some contaminants - e.g. samples from the Irish Sea tended to show higher PCN concentrations, whereas higher levels of PCBs were observed in some fish sampled off the coasts of northern France. Similarly, samples of mullet from the southeast coast of UK showed much higher concentrations of BDE-99 than the other regions. In terms of occurrence trends, PCDD/F and PCB concentrations show a modest decline over the last decade but where limited background data is available for emerging

  4. Apports en DHA (acide gras oméga-3 par les poissons et les fruits de mer consommés en France

    Directory of Open Access Journals (Sweden)

    Bourre Jean-Marie

    2007-01-01

    Full Text Available This present work measures the contributions of seafood (finfish and shellfish, wild and farmed to the intakes of DHA in France, year 2005, and consequently to the French recommended daily intakes (RDA of DHA. For this purpose, we measured the concentrations of DHA in each individual seafood by analysis of many published data. We also determined the exact consumption of the main products of seafood (fishing and farming in France (year 2005 using the modified method of the dietary intake measurement defined by the FAO. For year 2005, the 34 species of finfish and shellfish whose DHA contents are known account for 150% of the RDA of this omega-3 fatty acid. Taking all the species individually and extrapolating to include those whose DHA contents are not known, gives 156% of the RDA. The 10 greatest contributors to the DHA intake in the present diet are: salmon (47.6 mg/day/person, sardine (28.4, tuna (20.5, mackerel (15.7, herring (12.4, Alaskan pollock (8.9, cod (6.9, trout (6.5, hake (4.7, saithe (4.6. The 5 species of oily fish (salmon, sardine, tuna, mackerel and herring provide 63% of the DHA intake, and thus approx 95% of the French RDA. Besides DHA, seafood contains low amounts of ALA, but it can very high in farmed fish fed on rapeseed or linseed products. Omega-3 fatty acids (including EPA and DHA have an important role in human diet, both for prevention and the therapy of different pathologies. Data from many epidemiological studies has shown an inverse association between fish consumption, generally oily fish, and reductions in certain diseases. Although number of people do not lack dietary DHA, others, mainly pregnant women and nursing mothers and those people whose life styles or socio-economic positions restrict their seafood intakes, would benefit greatly from an increased intake of this omega-3 fatty acid.

  5. Chronic lack of breeding by Galápagos Blue-footed Boobies and associated population decline

    Directory of Open Access Journals (Sweden)

    David Anchundia

    2014-06-01

    Full Text Available A survey of Blue-footed Boobies (Sula nebouxii excisa throughout the taxon's range in Galápagos, Ecuador found ~6400 adults, compared to a rough estimate of 20,000 in the 1960s. Few pairs bred in 2011-2013 and almost no birds in juvenile plumage were seen. Long-term data suggest that poor breeding began in 1998. Lack of recruitment over this period would mean that the current population is mostly elderly and experiencing senescent decline in performance. Anthropogenic effects such as introduced predators are unlikely to explain this decline because islands with and without such factors exhibited the same low breeding. The poor reproduction seems to be linked to diet. Previous work indicated that sardine and herring (Clupeidae supported successful breeding, but these fish were mostly absent from the diet during this study, except in the central part of Galápagos, where most breeding attempts during this study occurred. Elsewhere in the eastern Pacific sardine abundance has decreased dramatically by natural processes in the last 15 years, as part of a well-documented and apparently natural cycle. This cyclic change in abundance provides a possible explanation for the recent demographic changes in Blue-footed Boobies in Galápagos. Whether natural or anthropogenic in origin, the implications of senescent decline in breeding ability and survival are dramatic for this genetically distinct icon of biodiversity and ecotourism.

  6. Analysis of the scallop microbiota by means of 16S rRNA gene pyrosequencing

    OpenAIRE

    Alex Mira; Jesus L Romalde

    2014-01-01

    Bivalve molluscs, due to its filter-feeding mechanism, have an abundant associated bacterial microbiota composed of a large number of species of different genera. The knowledge about the composition of the microbiota associated with bivalve molluscs is based, mostly, on techniques of cultivation of microorganisms, but also on DGGE methods. However, it is estimated that less than 1% of the bacteria are culturable with current methods, which lead to an underestimation of the microbial diversity...

  7. Analysis of the scallop microbiota by means of 16S rRNA gene pyrosequencing

    Directory of Open Access Journals (Sweden)

    Alex Mira

    2014-06-01

    Pyrosequencing of the samples resulted in a total of 18520 sequences (3000 per sample, approximately with an average length of 325 bp (base pairs. The taxonomic assignment of sequences allowed the identification to the genus level, being observed a large bacterial diversity with over 110 genera. The most prevalent genera in the samples were Hydrotalea, Acinetobacter, Delftia, Sediminibacter and Pseudomonas, among others. Differences in the microbial communities were observed among the samples, and the PCoA analysis allowed their separation by means on their gender and if they proceed from sampling before or after the spawning. Nevertheless, the rarefaction curves obtained for each sample failed to reach a saturation phase, indicating that more sequencing effort would be necessary.

  8. 78 FR 42890 - Fisheries of the Northeastern United States; Atlantic Sea Scallop Fishery and Northeast...

    Science.gov (United States)

    2013-07-18

    ...: Emily Gilbert, Fishery Policy Analyst, 978-281-9244; fax 978-281-9135. SUPPLEMENTARY INFORMATION... paragraph (b)(4) is revised to read as follows: Sec. 648.53 Acceptable biological catch (ABC), annual catch...

  9. 78 FR 27088 - Fisheries of the Northeastern United States; Atlantic Sea Scallop Fishery and Northeast...

    Science.gov (United States)

    2013-05-09

    ... Thomas Nies, Executive Director, New England Fishery Management Council, 50 Water Street, Newburyport, MA... associated with a 25-percent probability of exceeding the OFL. The Council's Scientific and Statistical... IFQ). In situations where a vessel leases out its IFQ to multiple vessels, only the vessel(s) that, in...

  10. Sperm density required for inducing gynogenetic haploidy in scallop Chlamys nobilis

    Digital Repository Service at National Institute of Oceanography (India)

    Goswami, U.

    Experiments conducted for standardizing sperm density for artificial insemination and inducing gynogenesis by ultra-violet rays irradiated sperms in Chlamys nobilis showed that 10:1 ratio of sperms to eggs, gave maximum rate of fertilization...

  11. Antibacterial activity in the hemolymph of the catarina scallop Argopecten ventricosus

    OpenAIRE

    Luna-González, Antonio; Maeda-Martínez, Alfonso; Campa-Córdova, Ángel; Orduña-Rojas, Javier

    2007-01-01

    We conducted a search for antibacterial peptide like activity in hemolymph of Argopecten ventricosus. Pre-purification of peptides was done by reverse phase HPLC. Hemolymph acidic supernatant was loaded into a column packed with C18 matrix. Stepwise elutions were performed with 5, 50, 80, and 100 % acetonitrile (ACN) in 0.05 % trifluoroacetic acid (TFA) over 550 min at a flow rate of 0.2 ml/min. Absorbance was monitored at 280 nm. Eluted fractions were concentrated under vacuum. Vibrio algino...

  12. Effect of temperature on physiological responses of Peruvian scallop Argopecten purpuratus

    Directory of Open Access Journals (Sweden)

    Jhon Dionicio Acedo

    2015-12-01

    Full Text Available The effect of temperature on the clearance rate (CR, ingestion rate (IR and specific oxygen consumption (SOC in individuals of Argopecten purpuratus at different size groups were determined. The CR and IR tests were performed at a concentration of approximately 1x106 cel.mlL-1 of Chaetoceros calcitrans, two temperatures 17 and 22 °C were evaluated and different groups of average size were formed of 7.6 ± 0.265, and 0.058 ± 4.9 3.7 ± 0.173 cm. In SOC test the average size groups were 8.1 ± 0.351, 0.058 ± 5.6 and 4.3 ± 0.100 cm. The results show a significant effect of temperature in CR (Lh-1 and IR (cel.h-1 x 105 on the larger individuals (7.6 ± 0.265 cm, it was observed at 22 °C an average increase, about 17 °C, up to 250% to CR and 48% to IR. In addition, a direct relationship of body size with CR and IR in both temperatures was observed. The effect of temperature at 22 ° C on SOC in all groups was evaluated, with an increase of 239.8, 165.3 and 183.4% for size individuals of 8.1 ± 0.351, 0.058 ± 5.6 and 4.3 ± 0.100 respectively. Furthermore, in both evaluated temperatures, the results show an indirect relationship of body size with the SOC.

  13. Extensive use of mesopelagic waters by a Scalloped hammerhead shark (Sphyrna lewini) in the Red Sea

    KAUST Repository

    Spaet, Julia L.Y.; Lam, Chi Hin; Braun, Camrin D.; Berumen, Michael L.

    2017-01-01

    This study provides evidence that mesopelagic habitats might be more commonly used by S. lewini than previously suggested. We identified deep diving behavior throughout the 24-h cycle over the entire 6-month tracking period. In addition to expected nightly vertical habitat use, the shark exhibited frequent mesopelagic excursions during daytime. Deep diving throughout the diel cycle has not been reported before and, while dive functionality remains unconfirmed, our study suggests that mesopelagic excursions may represent foraging events within and below deep scattering layers. Additional research aimed at resolving potential ecological, physiological and behavioral mechanisms underpinning vertical movement patterns of S. lewini will help to determine if the single individual reported here is representative of S. lewini populations in the Red Sea.

  14. 76 FR 23794 - Stock Status Determination for Atlantic Highly Migratory Scalloped Hammerhead Shark

    Science.gov (United States)

    2011-04-28

    ...) published in the North American Journal of Fisheries Management a stock assessment of the Atlantic... conservation and management measures to rebuild overfished stocks within 2 years of making this determination... to rebuilding the fishery within the shortest time possible in accordance with 16 U.S.C. 1854(e)(4...

  15. 77 FR 20728 - Fisheries of the Northeastern United States; Atlantic Sea Scallop Fishery; Framework Adjustment 23

    Science.gov (United States)

    2012-04-06

    ... to the proposed rule from: A representative from Nordic Fisheries, a family-owned company that runs... Nordic Fisheries generally supports the proposed measures in Framework 23, but commented that the final... regulatory language describing the TDD requirement. However, FSF continue to note their opinion that the TDD...

  16. Anti-predator adaptations in a great scallop (Pecten maximus) - a palaeontological perspective

    Science.gov (United States)

    Brom, Krzysztof Roman; Szopa, Krzysztof; Krzykawski, Tomasz; Brachaniec, Tomasz; Salamon, Mariusz Andrzej

    2015-12-01

    Shelly fauna was exposed to increased pressure exerted by shell-crushing durophagous predators during the so-called Mesozoic Marine Revolution that was initiated in the Triassic. As a result of evolutionary `arms race', prey animals such as bivalves, developed many adaptations to reduce predation pressure (e.g. they changed lifestyle and shell morphology in order to increase their mechanical strength). For instance, it was suggested that Pectinidae had acquired the ability to actively swim to avoid predator attack during the early Mesozoic. However, pectinids are also know to have a specific shell microstructure that may effectively protect them against predators. For instance, we highlight that the shells of some recent pectinid species (e.g. Pecten maximus) that display cross-lamellar structures in the middle part playing a significant role in the energy dissipation, improve the mechanical strength. In contrast, the outer layers of these bivalves are highly porous, which allow them to swim more efficiently by reducing the shell weight. Pectinids are thus perfect examples of animals optimising their skeletons for several functions. We suggest that such an optimisation of their skeletons for multiple functions likely occurred as a results of increased predation pressure during the so-called Mesozoic Marine Revolution.

  17. 50 CFR 648.60 - Sea scallop area access program requirements.

    Science.gov (United States)

    2010-10-01

    ... subsequent fishing year. For example, a vessel that terminates an Elephant Trunk Access Area trip on December...) Elephant Trunk Access Area. For the 2010 fishing year, the observer set-aside for the Elephant Trunk Access... Area. For the 2010 fishing year, the research set-aside for the Elephant Trunk Access Area is 277,060...

  18. 75 FR 36559 - Fisheries of the Northeastern United States; Atlantic Sea Scallop Fishery; Framework Adjustment 21

    Science.gov (United States)

    2010-06-28

    ... of the fishing year (FY). Framework 21 specifies measures only for FY 2010. Amendment 15 to the FMP... trips in the Elephant Trunk Access Area (ETAA), and one trip in the Delmarva Access Area (Delmarva). A... through October 31 into other areas and times of year when sea turtle interactions are less likely. This...

  19. Fortification of Sardine Fish Oil from By-product of Canning Processing into Beef Meatball and Chicken Nugget

    Directory of Open Access Journals (Sweden)

    Teti Estiasih

    2017-04-01

    Full Text Available One source of ω-3 fatty acids is a by-product of lemuru canning processing that can be used for fortification.  Generally, fortification uses fish oil microcapsule but it is more expensive than direct fortification.  In this study, fish oil from a by-product of lemuru canning processing was directly fortified into beef meatball and chicken nugget at concentration of 0, 2, 4, and 6% (w/w.  Oxidation level, free fatty acid content, colour, lightness, texture, and sensory acceptance by triangle difference test were analyzed. The results showed that oxidation level of nugget was higher than meatball.  Free fatty acid content increased in nugget by increasing fortification level, but it was decrease in meatball. Texture of both was relatively unchanged, with a tendency to increase in nugget and decrease in meatball. Lightness (L of meatball surface was higher than nugget surface. Lightness did not significantly change by increasing fortification level. Redness (+a and yellowness (+b of meatball and nugget changed significantly by fortification. Difference test showed that fortification level of 2% was the highest level of fortification that taste and odor could not be distinguished with control by panelists. Best level of fish oil fortification was 2%. At 2% fortification, EPA was 2.85% for meatball, and 2.22% for nugget.  Sharp decline was occured in EPA and the decrease washigher in nugget than meatball.

  20. Fortification of Sardine Fish Oil from By-product of Canning Processing into Beef Meatball and Chicken Nugget

    OpenAIRE

    Teti Estiasih; Endang Trowulan; Widya Dwi Rukmi

    2017-01-01

    One source of ω-3 fatty acids is a by-product of lemuru canning processing that can be used for fortification.  Generally, fortification uses fish oil microcapsule but it is more expensive than direct fortification.  In this study, fish oil from a by-product of lemuru canning processing was directly fortified into beef meatball and chicken nugget at concentration of 0, 2, 4, and 6% (w/w).  Oxidation level, free fatty acid content, colour, lightness, texture, and sensory acceptance by triangle...