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Sample records for sardine sardinella aurita

  1. A study of the life history of Brazilian sardines, Sardinella aurita: I. Distribution and abundance of sardine eggs in the region of Ilha Grande, Rio de Janeiro

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    Yasunobu Matsuura

    1971-06-01

    Full Text Available Distribution and abundance of sardine eggs in the Ilha Grande region, Rio de Janeiro, Brazil, were studied during five cruises for the 1969 1970 period. Using the shipboard fertilized eggs and the planktonic eggs, morphological descriptions of Brazilian sardine eggs are given. Spawning occurs during the summer from September to March in the coastal water off Ilha Grande to Ilha de São Sebastião down to a depth of 100 meters. Spawning may take place a few hours prior to midnight. lt was noticed that spawning has a close relationship to an area of cold water up welling. Spawning groups are isolated and spawning is small in scale in this region. Temperature and salinity in the spawning area range between 18-24ºC and 35.1-35.9‰ respectively.O presente trabalho apresenta um estudo da fase inicial do ciclo de vida da sardinha (Projeto SOL e verifica o seu potencial re produtivo ("spawning power" na costa sul do Brasil. Cinco viagens à região da Ilha Gran de foram realizadas com o N/Oc. " Prof. W. Besnard" e barco "Emilia". Os resultados estão resumidos, a seguir: 1. Os ovos sao esféricos e o espaço pe rivitelino é amplo. O diâmetro medio dos ovos e de 1,18 mm, cuja distribuição é de 1,00 - 1,32 mm. O espaço perivitelino mede, em média, 0,143 mm, com distribuição entre 0,061-0,254 mm. Geralmente o glóbulo de oleo é único, porém, às vezes apresenta-se segmentado em dois ou três. O seu diâmetro médio é de 0,140 mm, com distribuição entre 0,091 0,182 mm. 2. Podemos supor que a hora de desova da sardinha, nesta região, é pouco antes da meia-noite. Os ovos coletados numa mesma área e num mesmo dia, provenientes de um mesmo grupo de "desovantes", foram classificados em grupos de sardinhas que desovaram na área durante uma viagem de pesquisa. O tamanho dos cardumes de sardinha "desovante" não é grande como os da sardinha da Califórnia. 3. A área de desova, sôbre a plataforma continental, estende-se desde a Ilha de S

  2. Climate modulates the effects of Sardinella aurita fisheries off Northwest Africa

    NARCIS (Netherlands)

    Zeeberg, J.J.; Corten, A.; Tjoe-Awie, P.; Coca, J.; Hamady, B.

    2008-01-01

    The fluctuating abundance of round sardinella (Sardinella aurita) in Mauritanian waters over the past ca. 20 years can be related to environmental dynamics off Northwest Africa. Trends in the fishery are evaluated using FAO data, acoustic surveys, and catch statistics from the EU fleet (1996-2006).

  3. Biochemical and Functional Properties of Sardinella (Sardinella aurita By-Product Hydrolysates

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    Ali Bougatef

    2007-01-01

    Full Text Available Fish protein hydrolysates (FPHs with different degrees of hydrolysis (DH of 6.62, 9.31 and 10.16 were prepared from heads and viscera of sardinella (Sardinella aurita by treatment with Alcalase®. The liquid hydrolysates were spray dried. All spray dried by-product hydrolysates contained 73 to 75 % of proteins and low content of lipids. Reversed-phase HPLC profiles showed that low DH hydrolysates contained high hydrophobic peptides and the extent of hydrolysis resulted in an increase of fish hydrophilic peptides. Some functional properties of FPHs were assessed and compared with those of casein or the undigested sardinella protein. Solubility increased while emulsifying capacity and whippability decreased with the increase in the degree of hydrolysis. The product, with a DH of 10.16 %, had an excellent solubility (100 % over a pH range of 6.0–10.0. The antioxidant activity of the hydrolysates was also tested. All FPHs exhibited more than 50 % inhibition of linoleic acid peroxidation. The antioxidant activity of FPH with 10.16 % DH was about 73 % of that of α-tocopherol, a natural antioxidative agent.

  4. Fisheries management of round sardinella Sardinella aurita along North Sinai coast

    Institute of Scientific and Technical Information of China (English)

    Ahmed Mohamed Al-beak

    2016-01-01

    Objective:To estimate the biological and population parameters required for proposing a preparation to sustain and manage this valuable fish resource. Methods:Aging was done by scales reading, and growth was estimated by using the back-calculation method. The values of growth parameters L∞, k and to were calculated by von Bertalanffy model. Results: The results of growth parameters L∞, k and towere 28.36 cm, 0.184 per year and–0.843 7 per year, respectively. Mortality coefficient, survival and exploitation rates estimated to perceive yield per recruit and biomass per recruit. Conclusions: Biological reference points and virtual population analysis were prepared to plan appropriate managements forSardinella aurita fisheries.

  5. The link between migration, the reproductive cycle and condition of Sardinella aurita off Mauritania, north-west Africa

    NARCIS (Netherlands)

    Hofstede, ter R.; Dickey-Collas, M.; Mantingh, I.T.; Wague, A.

    2007-01-01

    The annual migration pattern of round sardinella Sardinella aurita up and down the north-western African coast between 12° N (Senegal) and 22° N (western Sahara) was shown to be associated with spawning activity and a distinct seasonality in fish condition, based on monthly sampling from commercial

  6. Insights on stock structure of round sardinella Sardinella aurita off north-west Africa based on otolith shape analysis.

    Science.gov (United States)

    Bacha, M; Jeyid, A M; Jaafour, S; Yahyaoui, A; Diop, M; Amara, R

    2016-10-01

    This study examines the geographic variability in otolith shape of round sardinella Sardinella aurita as a tool for stock discrimination. Fish were analysed from six sampling locations from Senegal to the Mediterranean coast of Morocco. A combination of otolith shape indices and elliptic Fourier descriptors was investigated by multivariate statistical procedures. Within the studied area, three distinct groups were identified with an overall correct classification of 78%. Group A: Nador (Alboran Sea), group B: Casablanca (northern Morocco) and group C: Senegalese-Mauritanian. The results of this study confirm the absence of an Atlantic Ocean-Mediterranean Sea transition for this species, the Gibraltar Strait acting as an efficient barrier for S. aurita population separation. Off north-west Africa, fish from northern Morocco form a single group which is clearly isolated from Senegalese-Mauritanian waters, confirming the existence of a distinct stock in this area. Among group C, some discontinuity exists and suggests the existence of a sedentary fraction of S. aurita in northern Mauritania (Arguin Bank). The results are discussed in relation to oceanographic features and physical barriers to dispersal and fish management strategy in the study area.

  7. Little genetic variation in the oil sardine, Sardinella longiceps Val., from the western coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Menezes, M.R.

    Enzyme gene variability in the oil sardine, @iSardinella longiceps@@, from three localities along the western coast of India was studied by starch-gel electrophoresis. Out of 19 loci scored, no locus was polymorphic by the 95% criterion. Seven loci...

  8. Hidden Diversity in Sardines: Genetic and Morphological Evidence for Cryptic Species in the Goldstripe Sardinella, Sardinella gibbosa (Bleeker, 1849)

    Science.gov (United States)

    Thomas, Rey C.; Willette, Demian A.; Carpenter, Kent E.; Santos, Mudjekeewis D.

    2014-01-01

    Cryptic species continue to be uncovered in many fish taxa, posing challenges for fisheries conservation and management. In Sardinella gibbosa, previous investigations revealed subtle intra-species variations, resulting in numerous synonyms and a controversial taxonomy for this sardine. Here, we tested for cryptic diversity within S. gibbosa using genetic data from two mitochondrial and one nuclear gene regions of 248 individuals of S. gibbosa, collected from eight locations across the Philippine archipelago. Deep genetic divergence and subsequent clustering was consistent across both mitochondrial and nuclear markers. Clade distribution is geographically limited: Clade 1 is widely distributed in the central Philippines, while Clade 2 is limited to the northernmost sampling site. In addition, morphometric analyses revealed a unique head shape that characterized each genetic clade. Hence, both genetic and morphological evidence strongly suggests a hidden diversity within this common and commercially-important sardine. PMID:24416271

  9. Hidden diversity in sardines: genetic and morphological evidence for cryptic species in the goldstripe sardinella, Sardinella gibbosa (Bleeker, 1849.

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    Rey C Thomas

    Full Text Available Cryptic species continue to be uncovered in many fish taxa, posing challenges for fisheries conservation and management. In Sardinella gibbosa, previous investigations revealed subtle intra-species variations, resulting in numerous synonyms and a controversial taxonomy for this sardine. Here, we tested for cryptic diversity within S. gibbosa using genetic data from two mitochondrial and one nuclear gene regions of 248 individuals of S. gibbosa, collected from eight locations across the Philippine archipelago. Deep genetic divergence and subsequent clustering was consistent across both mitochondrial and nuclear markers. Clade distribution is geographically limited: Clade 1 is widely distributed in the central Philippines, while Clade 2 is limited to the northernmost sampling site. In addition, morphometric analyses revealed a unique head shape that characterized each genetic clade. Hence, both genetic and morphological evidence strongly suggests a hidden diversity within this common and commercially-important sardine.

  10. A study of the life history of brazilian sardine, Sardinella brasiliensis.: III. development of sardine larvae

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    Yasunobu Matsuura

    1975-01-01

    Full Text Available Larvae and juveniles of S. brasiliensis (Steindachner, 1879, ranging from 6.4 mm to 35.5 mm, were identified from plankton samples taken in waters off the southern Brazilian coast from 1969 through 1971. Changes in the pattern of pigmentation, body proportions and formation of fin rays are described. During transformation stage a considerable advancement of the dorsal and anal fins was observed. Changes in body proportions are pronounced at the size of 19 mm. Complete ossification of all fin rays is attained at the size of 20 mm, but ossification of the ventral scutes is delayed and completed only at the size of 30 mm. Ossification of the vertebral column was completed at the size of about 16 mm.O presente trabalho é parte do projeto SOL e tem por objetivo descrever a morfologia das larvas da sardinha verdadeira, Sardinella brasiliensis (=S. aurita, com o intuito de determinar um padrão que possibilite a identificação das mesmas. São feitas considerações sobre a morfologia das larvas de outros clupeídeos existentes na região e ressaltadas as diferenças com relação as larvas de S. brasiliensis. Os resultados, posteriormente, serão usados como base para o estudo quantitativo da abundância de larvas de sardinha. O material foi coletado na costa sul do Brasil de 1969 a 1971, com uma rede de plâncton do tipo cônico-cilíndrico. Durante o desenvolvimento das larvas, foi observado um considerável deslocamento das bases das nadadeiras dorsal e anal, para uma posição mais anterior. Com o tamanho de 19 mm (comprimento padrão, ocorre uma mudança geral, considerável, nas proporções corporais. A ossificação de todos os raios das nadadeiras completa-se quando a larva atinge 20 mm, mas a ossificação dos escudos ventrais só se completa quando ela atinge 30 mm de comprimento. A ossificação das vertebras completa-se a 16 mm de comprimento. O tamanho de 19 mm foi considerado como o fim do estagio larval, e, apos este comprimento

  11. Spatial variability of helminth parasites and evidence for stock discrimination in the round sardinella, Sardinella aurita (Valenciennes, 1847), off the coast of Tunisia.

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    Feki, M; Châari, M; Neifar, L

    2016-05-01

    Three digeneans - Parahemiurus merus (Linton, 1910), Aphanurus stossichii (Monticelli, 1891) and Lecithochirium sp. - and one tetraphyllidean cestode larva were used as biological tags to discriminate the stock of Sardinella aurita (Valenciennes, 1847). In total, 579 fish were examined in five zones off the Tunisian coast, including Bizerte and Kelibia in the north, Mahdia in the east, Gabes and Zarzis in the south. Discriminant analyses used for the separation of S. aurita allowed for the identification of two discrete stocks. Sardinella aurita from Bizerte, Kelibia and Zarzis clumped together as a single stock. Parahemiurus merus and A. stossichii were the most important species in determining the location of sampled fish from these regions. Specimens from Mahdia and Gabes were grouped as one stock characterized by the presence of Lecithochirium sp. and larvae of the Tetraphyllidea. These results were corroborated by comparing the parameters of prevalence and mean abundance of parasites among zones. The separation of S. aurita between localities after pooling specimens from Bizerte, Kelibia and Zarzis and separately pooling those from Mahdia and Gabes also allowed the identification of two discrete stocks, one in offshore waters from Bizerte, Kelibia and Zarzis characterized by the digeneans P. merus and A. stossichii and one in inshore waters from Mahdia and Gabes characterized by Lechithochirium sp. and tetraphyllidean larvae.

  12. A study of the life history of brazilian sardine, Sardinella brasiliensis: II. spawning in 1970 and 1971

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    Yasunobu Matsuura

    1975-01-01

    Full Text Available Distribution and abundance of eggs of Sardinella brasiliensis (=S. aurita, are presented for the years 1970 and 1971 and compared with that of the previous year (1969. The spawning size of three spawning seasons was calculated using Tanaka's method. Incubation time of sardine eggs was estimated using the method of Ahlstrom. During three spawning seasons a considerable change in the relative spawning size was observed. The spawning season (during spring and summer in the southern hemisphere of 1970-71 was poor when compared with those of 1969-70 and 1971-72. A slight change in the average diameter of eggs in different spawning seasons was also observed. The relation between oceanographie conditions and spawning size was analysed. It is suggested that the warm water covering the spawning ground during the 1970-71 spawning season may have caused an unsuccessful spawning in this year.O presente trabalho apresenta a distribuição e abundância de ovos de sardinha verdadeira, Sardinella brasiliensis (=S. aurita, na costa sul do Brasil nos anos de 1970 e 1971. Um dos principais objetivos do estudo quantitativo de ovos e larvas de peixes é estimar o tamanho do estoque, através da abundância de ovos desovados. Com essa finalidade, a computação da abundância de ovos de sardinha verdadeira foi feita usando o método apresentado por Tanaka (1955. O tempo de incubação dos ovos de sardinha foi estimado com base no método da Ahlstrom (1943. A quantidade de ovos desovados variou durante os tres anos (1969 a 1971. Foi feita uma comparação entre a abundância total de ovos desovados e a freqüência de ocorrência de ovos nas estações da região estudada. Os índices de abundância mostram que a desova na época de 1970-71 foi muito fraca, comparada com as de 1969-70 e de 1971-72. Para esclarecer a relação entre a variação anual de tamanho da desova e a condição ambiental, foi analisada a temperatura e salinidade da água na area de desova. Os

  13. Production and characterization of refined oils obtained from Indian oil sardine (Sardinella longiceps).

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    Chakraborty, Kajal; Joseph, Deepu

    2015-01-28

    Crude Sardinella longiceps oil was refined in different stages such as degumming, neutralization, bleaching, and deodorization. The efficiency of these processes was evaluated on the basis of free fatty acid (FFA), peroxide (PV), p-anisidine (pAV), total oxidation (TOTOX), thiobarbituric acid reactive species (TBARS) values, Lovibond CIE-L*a*b* color analyses, and (1)H NMR or GC-MS experiments. The utilities of NMR-based proton signal characteristics as new analytical tools to understand the signature peaks and relative abundance of different fatty acids and monitoring the refining process of fish oil have been demonstrated. Phosphoric acid (1%) was found to be an effective degumming reagent to obtain oil with the lowest FFA, PV, pAV, TOTOX, and TBARS values and highest color reduction. Significant reduction in the contents of hydrocarbon functionalities as shown by the decrease in proton integral in the characteristic (1)H NMR region was demonstrated by using 1% H3PO4 during the course of the degumming process. A combination (1.25:3.75%) of activated charcoal and Fuller's earth at 3% concentration for a stirring time of 40 min was found to be effective in bleaching the sardine oil. This study demonstrated that unfavorable odor-causing components, particularly low molecular weight carbonyl compounds, could successfully be removed by the refining process. The alkane-dienals/alkanes, which cause unfavorable fishy odors, were successfully removed by distillation (100 °C) under vacuum with aqueous acetic acid solution (0.25 N) to obtain greater quality of refined sardine oil, a rich source of essential fatty acids and improved oxidative stability. The present study demonstrated that the four-stage refinement process of sardine oil resulted in a significant improvement in quality characteristics and nutritional values, particularly n-3 PUFAs, with improved fish oil characteristics for use in the pharmaceutical and functional food industries.

  14. Brazilian sardine (Sardinella brasiliensis spawning in the southeast Brazilian Bight over the period 1976-1993

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    Yasunobu Matsuura

    1998-01-01

    Full Text Available Based on sampling over the period 1976-1993 in the southeast Brazilian Bight, the distribution of spawning of the Brazilian sardine (Sardinella brasi/iensis is described in relation to environmental conditions. The area of intense spawning occurs in the southern part of the bight where coastal upwelling was less /Tequent. Spawning intensity showed high interannllal variation and the egg abundance in the survey area ranged /Tom 99 billion eggs in the January 1988 cruise to 4669 billion eggs in the January 1981 cruise. Peak spawning takes place one hour after midnight and eggs hatch . out within 19 hours with a water temperature of 24 °e.Baseado nos dados coletados durante nove cruzeiros oceanográficos realizados na região sudeste, as áreas de desova da sardinha-verdadeira (Sardinella brasiliensis foram apresentadas c discutidas em relação às condições oceanográficas. As áreas de desova intensiva foram localizadas na parte sul da área de investigação, onde a ressurgência costeira foi menos freqüente. A intensidade de desova demonstrou uma variação anual relativamente grande. A produção total de ovos da sardinha- ­verdadeira variou de 99 bilhões de ovos durante o cruzeiro de janeiro de 1988 para 4669 bilhões de ovos em janeiro de 1981. O pico de desova ocorre na camada de mistura de superfície uma hora após a meia noite e os ovos eclodem em 19 horas com a temperatura de água 24 °e.

  15. Effects of the addition of spleen of skipjack tuna (Katsuwonus pelamis) on the liquefaction and characteristics of fish sauce made from sardine (Sardinella gibbosa)

    OpenAIRE

    Klomklao, Sappasith; Benjakul, Soottawat; Visessanguan, Wonnop; Kishimura, Hideki; Simpson, Benjamin K.

    2006-01-01

    The effects of the addition of spleen of skipjack tuna (Katsuwonus pelamis), at levels of 0%, 10% and 20%, on the liquefaction and characteristics of fish sauce produced from the sardine (Sardinella gibbosa) with different salt concentrations (15%, 20% and 25%) were monitored during fermentation for 180 days. Fish sauces prepared from sardine with spleen supplementation contained greater total nitrogen, amino nitrogen, formaldehyde nitrogen and ammonia nitrogen than did those without spleen a...

  16. Heavy metals content by ICP-OES in Sarda sarda, Sardinella aurita and Lepidopus caudatus from the Strait of Messina (Sicily, Italy).

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    Lo Turco, Vincenzo; Di Bella, Giuseppa; Furci, Patrizia; Cicero, Nicola; Pollicino, Gianmichele; Dugo, Giacomo

    2013-01-01

    In this study copper, nickel, lead, cadmium, arsenic, chromium and vanadium content was measured in the muscles and gills of 24 fishes (Sarda sarda, Sardinella aurita and Lepidopus caudatus) caught in the Strait of Messina, by inductively coupled plasma-optical emission spectroscopy with microwave digestion techniques. In general, it was found that arsenic was higher than other analytes in all fish species, particularly its content was much higher in S. aurita than in the other two exemplary species. Lead and cadmium were always detected with values below the legal limits (CE no. 1881/2006 and subsequent modification CE no. 629/2008).

  17. Effets de deux hydrolysats de protéines de poisson (Sardina pilchardus et Sardinella aurita sur le transport inverse du cholestérol et le statut redox, chez le rat soumis à un régime enrichi en cholestérol |Effects of two fish protein hydrolysates (Sardina pilchardus and Sardinella aurita on reverse cholesterol transport and redox status, in rat fed a cholesterol-enriched diet

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    Nora Athmani

    2016-12-01

    Full Text Available Introduction. Several studies have reported that marine peptides have antioxidant effect. However, few have focused on their cholesterol-lowering effect. Objective. The purpose of this study was to evaluate the effects of sardine (Sardina pilchardus and sardinelle (Sardinella aurita protein hydrolysates on reverse cholesterol transport, and antioxidant status, in rat fed a cholesterol-enriched diet. Materials and methods. Eighteen male Wistar rats (350±15g were divided into three groups, and fed 20% casein and 1% cholesterol for 15 days. During this period, two groups received by gavage sardine protein hydrolysates (HPS or sardinelle (HPA solution. The 3rd group received water (GC. Results. Cholesterolemia was 1.7-fold lower in hydrolysates fed groups. The lecithin: cholesterol acyltransferase (LCAT activity was respectively, 1.7- and 1.6-fold higher in HPS and HPA groups. Phospholipids values of high density lipoprotein (PL-HDL were 1.4-fold lower in HPS compared with GC group. Cholesteryl esters amounts of HDL (CE-HDL were respectively, 1.7- and 1.5-fold higher in HPS and HPA groups. Liver and muscle thiobarbituric acid reactive substances (TBARS were respectively reduced by 68% and 29% in HPS and 48.5% and 63.6% in HPA group. Superoxide dismutase (SOD activity was increased by 32% in liver and 78% in muscle of HPA. Conclusion. It seems that HPS and HPA act favorably on reverse cholesterol transport, and improve oxidative stress, in rat fed cholesterol- enriched diet.

  18. Kannibalisme onder sardinella's

    NARCIS (Netherlands)

    Mantingh, I.T.

    2003-01-01

    Verslag van het RIVO onderzoek in Mauritanië naar de biologie van sardinella. Om de migratiepatronen van de Sardinella aurita in de Noord-west-Afrikaanse wateren te achterhalen zijn gegevens van Nederlandse trawlers en lokale kano's gebruikt. Ook ontdekte men kannibalisme onder sardinella's

  19. Kannibalisme onder sardinella's

    NARCIS (Netherlands)

    Mantingh, I.T.

    2003-01-01

    Verslag van het RIVO onderzoek in Mauritanië naar de biologie van sardinella. Om de migratiepatronen van de Sardinella aurita in de Noord-west-Afrikaanse wateren te achterhalen zijn gegevens van Nederlandse trawlers en lokale kano's gebruikt. Ook ontdekte men kannibalisme onder sardinella's

  20. Inventory and Characterization of Sardine (Sardinella Sp. Oil from Java Island-Indonesia

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    Sugeng Heri Suseno

    2014-05-01

    Full Text Available Parameter of fish oil quality could be observed from an omega-3 fatty acid content, antioxidant activity, Peroxide Value (PV, Free Fatty Acid (FFA value, total oxidation (totox value, density and viscosity, level of toxicity and its heavy metals content. This study aimed to determine the quality of sardine oils which was obtained as by product of fish meal and fish canning industry. Sardine A and B had different fatty acid composition. Based on toxicity test, it was known that sardine oil B (572.02 ppm had higher toxicity than sardine oil A (726.03 ppm. Heavy metal analysis results showed that sardine oil A had Pb concentration at 0.118 ppm, it passed a standard limit (≤0.1 ppm and it might be harmful for consumption. Peroxides value of sardine oil A and B was 13.33 meq/kg and 5.00 meq/kg, respectively. The highest FFA value was sardine oil B (3.948% then followed by sardine oil A (0.423%. Value of p-anisidine of sardine oil A and B was 1.09 and 0.88 meq/kg. The highest totox value was sardine oil A (27.76 meq/kg then followed by sardine oil B (10.88 meq/kg. Viscosity of sample B (270 cPs was higher than A (69 cPs and sample B (1.02 g/cm3 was denser than sample A (0.92 g/cm3.

  1. Structure and function of the retinal pigment epithelium, photoreceptors and cornea in the eye of Sardinella aurita (Clupeidae, Teleostei

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    Mostafa Ali Salem

    2016-05-01

    Full Text Available The structure of the pigment epithelium, photoreceptors and the cornea in the eye of a teleost, Sardinella aurita was examined by light and electron microscopy. The retinal pigment epithelium forms a single layer of cells joined laterally by cell junctions. Centrally in the retina these cells are columnar, while more peripherally they become cuboidal in shape. The basal (scleral border of the pigment epithelial cells is not infolded but is relatively smooth. Phagosomes containing lysosome-like bodies are also common features of the retinal pigment epithelium. Numerous melanosomes (pigment granules are abundant throughout the epithelial cells. These melanosomes probably absorb light which has passed through the photoreceptor layer. Four photoreceptor cells were identified; rods, long single cones, short single cones and double cones. The presence of these types suggests a diversity of photoreceptor function. Square mosaic pattern of cones and well-developed choroid gland are also main features of the eye. The inner segment of rods and cones were rich in organelles indicating much synthetic activity. Calycal processes projecting from cone outer segments are also observed. The cornea includes an epithelium with a complex pattern of surface microplicae, a basement membrane, dermal stroma, an iridescent layer, scleral stroma, Descemet’s membrane and endothelium. The autochthonous layer which is seen in some teleosts has not been observed in the cornea of this species. These and other observations were discussed in relation to the photic environment and habits of this fish.

  2. Genetic relationship of Sardinella lemuru from lombok strait with fish rich in omega-3 fatty acid.

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    Mahrus; Sumitro, Sutiman Bambang; Utomo, Didik Huswo; Sartimbul, Aida; Toha, Abdul Hamid; Widodo, Nashi

    2012-01-01

    Lombok Strait has abundance of Sardine, Sardinella lemuru, which contains such high amount of omega-3 fatty acid (omega-3). However, the genetic relationship of S. lemuru with other commercial fish rich in omega-3 has not been widely studied yet. Studies on genetic proximity of S. lemuru with the other marine fish using 12S rRNA gene is very important in order to obtain genetic information of the Sardine to develop an appropriate strategy for future conservation of the fish in Lombok Strait. The aim of this study was to find out the genetic relationship of Sardinella lemuru living in Lombok Strait with the economically valuable fish and its correlation with omega-3 production. Sardinella lemuru were collected from Lombok Strait, the phylogenetic tree was done based on 12S rRNA gene through a neighbor-joining method to identify the relationship of Sardines and fish rich in omega-3 fatty acid. The phylogenetic tree showed that Sardinella lemuru is similar to Sardinella aurita and has a close similarity with Sardinella maderensis. However, the relationship did not correspond to omega-3 production. Based on the results of the study, it is suggested that the production of omega-3 is not specifically based on the proximity of the species, but it is more associated with conserved domain of Δ6-desaturase. Nevertheless, detailed mechanisms still need to be elucidated.

  3. Draft Genome Sequence of Aeromonas dhakensis Strain F2S2-1, Isolated from the Skin Surface of an Indian Oil Sardine (Sardinella longiceps).

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    Nadiga, Mohan; Vaidyanathan, V V; Thayumanavan, Thangavelu

    2016-08-18

    Draft genome sequencing of Aeromonas dhakensis strain F2S2-1, isolated from the skin surface of an Indian oil sardine (Sardinella longiceps), has been carried out. The draft genome was roughly 4.7 Mb in size with 61.7% G+C content. Annotation of the genome yielded 4,337 genes coding for proteins, tRNAs, and rRNAs. Annotation also revealed the presence of 52 genes linked to resistance to antibiotics/toxic compounds. Pathway analysis revealed the presence of novobiocin biosynthetic genes and genes for biosynthesis of a siderophore group on nonsynthetic peptides. Copyright © 2016 Nadiga et al.

  4. Baja diferenciación genética entre poblaciones de sardina, Sardinella aurita, del oriente venezolano

    Directory of Open Access Journals (Sweden)

    2005-01-01

    Full Text Available Debido a la gran importancia de Sardinella aurita como recurso pesquero de la región del Caribe, se estudió la diferenciación genética entre las poblaciones de Mochima, Golfo de Cariaco y Morro de Puerto Santo, en el oriente de Venezuela, y se compararon los niveles de variación y subdivisión poblacional con las poblaciones del Mediterráneo y la costa Atlántica de África, anteriormente reportadas. Para esto, se obtuvieron 15 individuos de cada una de tres poblaciones, utilizando una muestra de músculo para extraer el ADN total. Se amplificó un fragmento que incluye la región de control del ADN mitocondrial y se determinaron los patrones de restricción por medio del análisis por RFLP con las enzimas Taq I, Eco RI, Hae III y Alu I. La amplificación de esta región produjo un fragmento de aproximadamente 1000 bp o, rara vez, uno de 1100 pb. El análisis de RFLP demostró la presencia de sólo un haplotipo con las dos variantes de tamaño. El FST resultante del análisis de las diferencias entre poblaciones venezolanas fue menor de 0.06 y el AMOVA demostró que no existían diferencias significativas entre ellas. Estos resultados concuerdan con la presencia de poca diferenciación genética entre estas poblaciones, por lo que no se puede descartar la presencia de una población panmíctica en esta región. Una comparación de las poblaciones venezolanas con las poblaciones del Mediterráneo y África, reportadas con anterioridad, produjeron valores de FST muy bajos entre las venezolanas y las del Mediterráneo, mientras que las comparaciones entre las de Mauritania, Senegal, Costa de Marfil y Ghana produjeron valores de FST diez veces mayores.

  5. Fluctuations in the spawning stock biomass and recruitment of the brazilian sardine (Sardinella brasiliensis 1977-1997

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    Maria Cristina Cergole

    2002-01-01

    Full Text Available In the southeastern coast of Brazil, catches of the Brazilian sardine (Sardinella brasiliensis have been decreasing from more than 200 thousand tons/year in the early '70s to about 32 thousand tons in 1990. From 1994 to 1997, catches recovered slightly (118 thousand tons, dropping then to about 20 thousand tons in 1999-2000. Extensive investigations on biology and stock assessment of Brazilian sardine have been developed in the '80s, providing stock biomass and recruitment estimates based on commercial fishing data obtained from 1977-1992. The present paper aims to update biological and stock assessment parameters from sardine data obtained during two acoustic surveys carried out in 1995 and commercial catches from the 1993-1997 period. Results from both cruises showed the weight-length relationship Wt=0.0000019. Lt3,26 (90-240 mm total length and four age classes (mostly class II+. In the commercial catches, sardines belonged to ages 0+ and 3+ years old (mostly older than 1-year old with 90-250 mm total length. Annual growth parameters were: Li=275 mm, K= 0.55 (1996, and Li=273 mm, K=0.50 (1997. Total mortality (Z and natural mortality (M coefficients were: Z= 3.8; M= 0.7 -1.2 (1996, and Z= 3.6; M= 0.6 - 1.1 (1997. The variation amplitude of M is due to the application of several different methods. Considering the historical series of 21 years of data (1977-1997, results showed that the stock went through two favorable periods (1980-84, 1989-94 and one unfavorable period (1985-89. 1997 seems to have been the beginning of a new unfavorable period, revealing recruitment cycles likewise other stocks from California and Japan.Na costa sudeste do Brasil, as capturas da sardinha-verdadeira {Sardinella brasliensis decresceram de mais de 200 mil t/ano no início dos anos 70 para cerca de 32 mil t em 1990. De 1994 a 1997, as capturas se recuperaram alcançando 118 mil t, caindo então para cerca de 20 mil t em 1999-2000. Estudos abrangentes sobre a

  6. Monitoring the sardine (Sardinella brasiliensis fermentation process to obtain anchovies Monitoramento do processo de fermentação da sardinha, Sardinella brasiliensis, para obtenção de anchovas

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    Marília Oetterer

    2003-01-01

    Full Text Available Anchovies are traditional fish preserves, prepared from fermented fish of the engraulidae family, mainly in European countries. In Brazil, sardines (Sardinella brasiliensis are an alternative fish for preparing these types of preserves, provided that the preservation process results in a high quality product. In this research, sardines were prepared for preservation and physicochemical, microbiological and sensory analyses were carried out during the preservation process. Whole or eviscerated sardines, with or without condiments/preservatives and with 20% of salt (w/w were used. Sardines were analyzed fresh, and at 1, 15, 30, 45 and 60 days along the preservation process. The use of whole sardines, with or without condiments/preservatives, presented best results, with increased non-proteic nitrogen in the dry matter, higher levels of total volatile bases and higher contents of lactic acid and sodium chloride. The higher acidity observed in the whole sardine treatments resulted in better control of halophylic mesophilic microorganisms, which were kept under 1.4 x 10³ CFU g-1 in both treatments. Total coliforms and Staphylococcus aureus reached 21 and 3.0 x 10² CFU g-1, respectively. Escherichia coli and Salmonella spp were not present in the fresh sardines or in any of the four treatments, indicating that the concentration of salt used was appropriate to maintain the product under adequate microbiological control. Both whole or eviscerated sardines under the conditions of this experiment were appropriate in terms of the microbiological safety of the preserves. Treatments using whole fish, either with or without condiments/preservatives, also presented better sensorial properties such as color, flavor, taste and texture, as compared to the eviscerated fish treatments. Whole sardines produced good quality, anchovy-type preserves, which can be used for consumption and marketing purposes.As sardinhas brasileiras podem ser utilizadas para o preparo

  7. Influence of smoking and packaging methods on lipid stability and microbial quality of Capelin (Mallotus villosus) and Sardine (Sardinella gibossa)

    Science.gov (United States)

    Cyprian, Odoli O; Van Nguyen, Minh; Sveinsdottir, Kolbrun; Jonsson, Asbjorn; Tomasson, Tumi; Thorkelsson, Gudjon; Arason, Sigurjon

    2015-01-01

    Lipid and microbial quality of smoked capelin (two groups differing in lipid content) and sardine was studied, with the aim of introducing capelin in the smoked sardine markets. Lipid hydrolysis (phospholipid and free fatty acids) and oxidation index (hydroperoxides and thiobarbituric acid-reactive substances), fatty acid composition, and total viable count were measured in raw and packaged smoked fish during chilled storage (day 2, 10, 16, 22, 28). Lipid hydrolysis was more pronounced in low lipid capelin, whereas accelerated lipid oxidation occurred in high lipid capelin. Muscle lipid was less stable in sardine than capelin. Essential polyunsaturated fatty acids (eicosapentaenoic acid and docosahexaenoic acid) constituted 12% of fatty acids in capelin and 19% in sardine. Vacuum packaging as well as hot smoking retarded bacterial growth, recording counts of ≤log 5 CFU/g compared to ≥log 7CFU/g in cold smoked air packaged. Smoked low lipid capelin was considered an alternative for introduction in smoked sardine markets. PMID:26405526

  8. Prevalance and pathology of gastric tumours in Indian oil sardine (Sardinella longiceps) from Parangipettai coastal waters, southeast coast of India

    Institute of Scientific and Technical Information of China (English)

    Vijayapoopathi Singaravel; Ayyaru Gopalakrishnan; RamalingamVijayakumar; Kuzhanthaivel Raja

    2015-01-01

    Objective: To carry out the survey of prevalence of gastric tumour in Sardinella longiceps of Parangipettai coastal waters, south east coast of India for a period of one year. Methods: Fish samples were directly collected from fishermen and also from auction yard. The prevalence of gastric tumour, gross pathology, radiography, histopathology, morphometric and meristic characters were investigated. Results: A total of 31 stomach tumour infected individuals were collected during the study period. The gross morphology showed distended abdomen and the radiograph exhibited enlargement of stomach. Autopsy of the infected fish exhibited reddish multilobed tumourous growth on the stomach. Histologically, the tumour lesions were characterized by the differential rate of glandular epitheloid and mesenchymal cells, polymorphic and hyperchromatic nuclei and mitotic activity. No evidence of local invasion and distinct metastases were observed in these cases. Conclusions: The tumours were diagnosed as gastric adenoma, myofibroblastoma, lipoma and fibrosarcoma. Among them myofibroblastoma is highly prevalent.

  9. Avaliação da utilização de embalagem em atmosfera modificada sobre a conservação de sardinhas (Sardinella brasiliensis Evaluation of the use of modied atmosphere packaging in sardine (Sardinella brasiliensis preservation

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    Anderson Junger Teodoro

    2007-03-01

    Full Text Available A vida útil dos alimentos perecíveis conservados em atmosfera normal é limitada principalmente pelo efeito do oxigênio atmosférico e o crescimento de microorganismos aeróbios produtores de alterações, que promovem mudanças de odor, sabor, cor e textura, conduzindo à perda da qualidade. A modificação da atmosfera prolonga significativamente a vida útil dos alimentos, quando comparados à refrigeração, podendo chegar a um aumento de três a quatro vezes. O trabalho teve como objetivo avaliar o efeito da embalagem de sardinhas (Sardinella brasiliensis em atmosfera modificada. As amostras foram divididas em 8 lotes, embaladas com aproximadamente 1,5 L de ar (100%, CO2 (100%, 50/50 CO2/O2 e vácuo e termo-seladas, sendo armazenadas por um período de 22 dias. A cada 2 dias as amostras foram avaliadas quanto ao pH, bases voláteis totais (BVT e contagens totais de microrganismos heterotróficos aeróbios mesófilos. Os valores de pH aumentaram durante o tempo de estocagem, com exceção das amostras armazenadas em 100 e 50% CO2. A evolução de BVT foi compatível com outros parâmetros, como pH e contagem de bactérias totais, sendo sua evolução mais lenta nas amostras em que ocorreu o uso de atmosfera modificada. Os microrganismos mesófilos, nas atmosferas enriquecidas com CO2, apresentaram fases de latência e tempos de duplicação maiores, quando comparados com os das amostras em que não foi aplicado o método. Sob o ponto de vista microbiológico, as embalagens enriquecidas com CO2 demonstraram ser o melhor método de conservação, como também os resultados de BVT se encontraram dentro dos limites de 30 mg.100 g -1 de acordo com o estabelecido pela RIISPOA8. Recomenda-se o uso da atmosfera de 100% de CO2 como forma de conservação da sardinha por apresentar melhores parâmetros de vida útil.The objective of the work was to evaluate the effect of sardines (Sardinella brasiliensis packaged in a modified atmosphere. The

  10. HISTAMINE IN CANNED SARDINES HISTAMINA EM CONSERVAS DE SARDINHA

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    Fancislene Bernardes Tebalti do Carmo

    2010-04-01

    Full Text Available In this study, the presence of histamine in 122 samples of canned sardines produced with three different species by three industries located in the municipalities of Sao Goncalo and Niteroi was evaluated. The samples were divided into five lots with copies of sardines from Venezuela (Sardinella aurita, Morocco (S. pilchardus and Brazil (S. brasiliensis. The initial quality of raw material was evaluated by sensorial parameters and by the histamine level using a semi-quantitative method of thin-layer chromatography. The results of the samples from Venezuela and Morocco showed values below 5 mg/100g, and the national samples showed values similar or greater than 10 mg/100g. It follows that there is need for greater control and monitoring of temperature from capture to processing, to guarantee good quality to the final product, and to avoid risk of poisoning to the consumer.

    KEY WORDS: Canned fish, histamine, quality, sardines.

    O presente estudo avaliou a presença de histamina em 122 amostras de sardinha em conserva, produzidas com três diferentes espécies, por três indústrias, localizadas nos municípios de São Gonçalo e Niterói. As amostras foram divididas em cinco lotes com exemplares de sardinhas provenientes da Venezuela (Sardinella aurita, Marrocos (S. pilchardus e do Brasil (S. brasiliensis. Avaliou-se a qualidade inicial da matéria-prima por meio de parâmetros sensoriais e pelo teor de histamina utilizando-se o método de cromatografia em camada delgada. As amostras oriundas da Venezuela e Marrocos apresentaram valores abaixo de 5 mg/100 g e as nacionais, valores semelhantes ou superiores a 10 mg/100g. Conclui-se que há necessidade de um maior controle e monitorização da temperatura da sardinha desde a captura até o processamento, para que o produto final apresente boa qualidade e não represente perigo de intoxicação ao consumidor.

    PALAVRAS-CHAVES: Conserva, histamina, qualidade, sardinha.

  11. [Design of a HACCP plan for the industrial process of frozen sardines].

    Science.gov (United States)

    Rosas, Patricia; Reyes, Genara

    2009-09-01

    The Hazard Analysis and Critical Control Point (HACCP) is a system to identify, assess and control the hazards related with production, processing, distribution and consumption in order to get safe food. The aim of this study was to design a HACCP plan for implementing in processing line of frozen whole sardine (Sardinella aurita). The methodology was based in the evaluation of the accomplishment of the pre-requisite programs (GMP/SSOP in a previous study), the application of the principles of the HACCP and the sequence of stages settles down by the COVENIN Venezuelan standard No 3802. Time-temperature was recorded in each processing step. Histamine was determined by VERATOX NEOGEN. Results showed that some sardine batches arrived to the plant with high time-temperature records, finding up to 5 ppm of histamine due to the abuse of temperature during transportation. A HACCP plan is proposed with the scope, the selection of the team, the description of the product and the intended use, the flow diagram of the process, hazard analysis and identification of CCP, monitoring system, corrective actions and records. The potential hazards were identified as pathogen growth, presence of histamine and physical objects in the sardines. The control measures of PCC are referred as control of time-temperature during transportation and processing, monitoring of ice supplies and sanitary conditions in the process.

  12. Integrative approach revises the frequently misidentified species of Sardinella (Clupeidae) of the Indo-West Pacific Ocean.

    Science.gov (United States)

    Stern, N; Rinkevich, B; Goren, M

    2016-11-01

    To deal with the difficulties of species differentiation and delimitation among the commercially important sardines from the genus Sardinella, an integrative approach was adopted, incorporating traditional taxonomy with four DNA markers (coI, cytb, 16s and nuclear rag2). Combining these methodologies has enabled a thorough re-description of three of the most common species of Sardinella of the Indo-west Pacific Ocean: white sardinella Sardinella albella, fringescale sardinella Sardinella fimbriata and the goldstripe sardinella Sardinella gibbosa, as well as a description of a new species, Gon's sardinella Sardinella goni, from the island of Boracay, Philippines. In addition, extensive widespread sampling of S. gibbosa reveals a significant genetic separation between the populations from the western Indian Ocean and the west Pacific Ocean, despite no supporting morphological differentiation. An updated morphological key of the species of Sardinella of the Indo-west Pacific Ocean is also provided in order to minimize future misidentifications within these economically important taxa. Finally, the genetic and morphological variabilities within and between the investigated species are used to discuss their biogeographical distribution and possible processes of speciation. © 2016 The Fisheries Society of the British Isles.

  13. [Evaluation of prerequisites programs for a HACCP plan for frozen sardine plant].

    Science.gov (United States)

    Rosas, Patricia; Reyes, Genara

    2008-06-01

    Good manufacturing practices (GMP) and sanitation standard operating procedures (SSOP) are prerequisites programs for the application of the Hazard Analysis and Critical Control Point (HACCP) system as a food safety approach during processing. The aim of this study was to evaluate GMP/SSOP prerequisites in processing line of frozen whole sardine (Sardinella aurita). The GMP compliance was verified according to a standard procedure of the Ministry for the Health of Venezuela, and the SSOP were assessed according to a checklist proposed by the FDA. GMP and SSOP were evaluated following a demerit-based approach. A percentage value was calculated and referred to as sanitary effectiveness. Results indicated that the plant had a good level of compliance with GMP from assessment of buildings and facilities, equipment and tools, hygienic requisites of the production, assurance of the hygiene quality, storage and transportation, and the percentage of sanitary effectiveness was 84%. The level of compliance for SSOP was 53,12% with demerits found in all assessed aspects consisting of inexistent guidelines, lack of control in the sanitary plan and lack of leadership in applying corrective actions. Thus, an improvement in the plant sanitation program was designed targeting SSOP.

  14. Prevalence of Nerocila depressa (Isopoda, Cymothoidae) on Sardinella albella from a Thai estuary

    Science.gov (United States)

    Printrakoon, Cheewarat; Purivirojkul, Watchariya

    2011-02-01

    The isopod Nerocila depressa is present on 54% of the market-sized white sardines ( Sardinella albella) caught from an estuary in the Trat province of Thailand. The ventral part of the body, especially the upper pectoral fin, was the area most frequently infected by this parasite. Parasite length was positively correlated with fish length, which suggests that the parasites attach to the fish at a young age. At the site of attachment, the parasites erode the epidermis, dermis and muscular tissue of the Sardinella. Such pathology may cause mortality in many fish. Infestation of this parasite was proportional to the weight of the fish. This study reports a new host for N. depressa attachment and is the first report of S. albella infestation in the Trat province where other known host fish species are cultured.

  15. Climate, Anchovy, and Sardine

    Science.gov (United States)

    Checkley, David M.; Asch, Rebecca G.; Rykaczewski, Ryan R.

    2017-01-01

    Anchovy and sardine populated productive ocean regions over hundreds of thousands of years under a naturally varying climate, and are now subject to climate change of equal or greater magnitude occurring over decades to centuries. We hypothesize that anchovy and sardine populations are limited in size by the supply of nitrogen from outside their habitats originating from upwelling, mixing, and rivers. Projections of the responses of anchovy and sardine to climate change rely on a range of model types and consideration of the effects of climate on lower trophic levels, the effects of fishing on higher trophic levels, and the traits of these two types of fish. Distribution, phenology, nutrient supply, plankton composition and production, habitat compression, fishing, and acclimation and adaptation may be affected by ocean warming, acidification, deoxygenation, and altered hydrology. Observations of populations and evaluation of model skill are essential to resolve the effects of climate change on these fish.

  16. Abundance of sardine fish species in Bangladesh

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    Roy Bikram Jit

    2012-06-01

    Full Text Available The study was conducted during January, 2012 to December 2012 in the sardine fisheries which is occurred both in artisanal and industrial fishing sector in the marine water of the Bay of Bengal of Bangladesh region. During this study period the total landing amounts by weight of sardines were 7352.99 MT, among these 23.76% (1747.22 MT was exploited by the artisanal mechanized boats and 76.24% (5605.77 MT captured through different industrial fishing trawlers and contributed 17.51% of the total marine fish production by commercial fish trawlers during the study period. 4 sardine species have been recorded from our marine territory. Among them, 2 sardine species are highly abundant, Sardinella fimbriata total production volumes was 5495.79 MT (74.74% contributed 1747.22MT (31.79% from the artisanal and 3748.57MT (68.21% from the industrial sector and Dussumieria acuta production amounts was 1857.20MT (25.26% contributed only from the industrial fishing sector.Species wise contribution shows that S. fimbriata contributed 100% in the artisanal sector and in the industrial fishing S. fimbriata contributed 66.87% and D. acuta contributed the rest 33.13%. The distribution of the S. fimbriata is within 10-20 meters depth and abundance was observed in the southern part of the South patches and South of south patches (N: 210.09// -22, E: 920.04/-07 to N: 200.45/-25, E: 920.18/-56 and 10-50m depth in onshore and off shore areas in the north-west to north-east of Middle ground (Kohinoor point -N: 210.36/.23, E: 900.06/.43 to N: 210.18/.18, E 910.17/.57. The distribution of the D. acuta is within 40-60 m. depth and abundance was observed in the north-west to north-east of Middle ground areas (Kohinoor point - N: 210.36/.23, E: 900.06/.43 to N: 210.18/.18, E 910.17/.57 and south-west to south-east of Middle ground (Kohinoor point- N: 200-17/.29, E: 900.15/.21 to N: 200.29/.56, E: 910.24/.22 in the Bay of Bengal of Bangladesh region. The peak capture season of

  17. Calcium and phosphorus determination in bones of low value fishes, Sardinella longiceps (Valenciennes and Trichiurus savala (Cuvier, from Parangipettai, Southeast Coast of India

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    Logesh AR

    2012-05-01

    Full Text Available Objective: To investigate calcium and phosphorus levels in the bones of low value fishes Sardinella longiceps (oil sardine and Trichiurus savala (ribbon fish. Methods: Bones and skeletal remains were subjected to alkaline-alcohol treatment for specified period of time and were then dried and pulverized. Calcium and phosphorus levels were determined by both volumetric and instrumental methods using atomic absorption spectroscopy. Results: Volumetric analysis of calcium and phosphorus were 28.98% and 14.2% in oil sardine; 24.2% and 11.6% in ribbon fish respectively. Atomic absorption spectroscopy analysis of calcium and phosphorus were 32.73% and 17.2% for oil sardine and 27.17% and 10.83% for ribbon fish respectively. Protein level was 4.82% in oil sardine and 3.97% in ribbon fishes. Conclusions: The findings of the present study revealed that sardine bones are rich in calcium and will be utilized for the production of calcium powder to treat osteoporosis.

  18. Assessing the quality of sardine based on biogenic amines using a fuzzy logic model.

    Science.gov (United States)

    Zare, Davood; Ghazali, H M

    2017-04-15

    There is an increasing concern about the quality and quality assessment procedures of seafood. In the present study, a model to assess fish quality based on biogenic amine contents using fuzzy logic model (FLM) is proposed. The fish used was sardine (Sardinella sp.) where the production of eight biogenic amines was monitored over fifteen days of storage at 0, 3 and 10°C. Based on the results, histamine, putrescine and cadaverine were selected as input variables and twelve quality grades were considered for quality of fish as output variables for the FLM. Input data were processed by rules established in the model and were then defuzzified according to defined output variables. Finally, the quality of fish was evaluated using the designed model and Pearson correlation between storage times with quality of fish showed r=0.97, 0.95 and 1 for fish stored at 0, 3 and 10°C, respectively. Copyright © 2016. Published by Elsevier Ltd.

  19. Bacteriocinogenic effect of Lactobacillus sakei 2a on microbiological quality of fermented Sardinella brasiliensis

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    Milton L. P. Espírito Santo

    2003-12-01

    Full Text Available Lactobacillus sakei 2a is a bacteriocin producer strain and, in this work, it's effects as a starter culture in the fermentation process of sardine (Sardinella brasiliensis fillets were observed at different concentrations of NaCl (2, 4 and 6% and glucose (2 and 4%, to determine it's ability to produce organic acids and consequent pH reduction. Experiments were carried out independently, with only one parameter (NaCl or glucose varying at a time. After 21 days of fermentation the deteriorative bacteria concentration reached 9.7 Log10 CFU. g-1 corresponding to 6% NaCl and 4% glucose. Little differences were observed in lactic acid production when 2 and 4% glucose were added, since total acidity was 1.32 and 1.34% respectively, the experiments with 6% NaCl presented the best results. Initial pH of sardine fillets was 6 and after 21 days pH values were 3.8, 3.9 and 4 for the experiments with 2, 4 and 6% NaCl. This may have been due to the inhibitory properties of NaCl over the deteriorative bacteria. After 21 days of the fermentation process lactic acid bacteria concentrations were 14.5 Log10 CFU.g-1. The ratio protein nitrogen and total soluble nitrogen was typical of a cured fish.Lactobacillus sakei é comprovadamente uma cepa produtora de bacteriocinas e, neste trabalho procurou-se observar seus efeitos como cultivo iniciador, na fermentação de filés de sardinha-verdadeira (Sardinella brasiliensis em diferentes concentrações de NaCl (2, 4 e 6% e glicose (2 e 4%, observando-se sua capacidade para produzir ácidos orgânicos e conseqüente redução do pH e microrganismos deterioradores. Os tratamentos foram desenvolvidos de forma independente, variando apenas um dos parâmetros operacionais (NaCl ou glicose. Ao término de 21 dias de fermentação, a concentração de microrganismos deterioradores atingiu 9,7 Log10 UFC.g-1, correspondente a 6% de NaCl e 4% de glicose. Poucas diferenças foram observadas na produção de ácido lático quando

  20. Mitochondrial DNA phylogeography of least cisco Coregonus sardinella in Alaska.

    Science.gov (United States)

    Padula, V M; Causey, D; López, J A

    2017-03-01

    This study presents the first detailed analysis of the mitochondrial DNA diversity of least cisco Coregonus sardinella in Alaska using a 678 bp segment of the control region (D-loop) of the mitochondrial genome. Findings suggest that the history of C. sardinella in Alaska differs from that of other species of Coregonus present in the state and surrounding regions. The examined populations of C. sardinella are genetically diverse across Alaska. Sixty-eight distinct mitochondrial haplotypes were identified among 305 individuals sampled from nine locations. The haplotype minimum spanning network and phylogeny showed a modest level of geographic segregation among haplotypes, suggesting high levels of on-going or recent connectivity among distant populations. Observed ΦST values and the results of homogeneity and AMOVAs indicate incipient genetic differentiation between aggregations in three broad regional groups. Sites north of the Brooks Range formed one group, sites in the Yukon and Selawik Rivers formed a second group and sites south of the Yukon drainage formed the third group. Overall, the sequence data showed that a large proportion of mtDNA genetic variation in C. sardinella is shared across Alaska, but this variation is not homogeneously distributed across all regions and for all haplotype groups.

  1. Estudo da diversidade de Sardinella brasiliensis (Steindachner, 1879, na área entre Macaé (22º23'S e Ilha de Santa Catarina (27º35'S: 1. Crescimento de dimensões corporais

    Directory of Open Access Journals (Sweden)

    Francisco Manoel de Souza Braga

    1987-01-01

    Full Text Available The growth of body proportions of Sardinella brasiliensis was not homogeneous in the study area, between Macaé (RJ and Santa Catarina Island (SC; the growth rates (K of the von Bertalanffy growth curve were greater than growth rates for another áreas. In this area the biomass estimates for pelagic fish is poor, and near Cabo Frio occurs an upwelling zone. These facts permit the sardines to attain large lengths in this area. Between Rio de Janeiro and Florianópolis the growth rates were lower than in the northern part and Paranaguá Bay. On the other hand the biomass estimates for pelagic fish in Rio de Janeiro-Florianópolis region was very high, and in this zone the population density is high and the growth rates are small. Paranaguá Bay is a particular region because of its geographical and oceanographic conditons; this is a shelter region with different features and the sardines living there attain small length. In this area the growth rates are intermediary among growth rates for fishes from Macaé-Cabo Frio and Rio de Janeiro-Florianópolis regions. Therefore, the growth rates for sardines in southeast Brazilian proves to be dependent of the population density, food and oceanographic conditions.

  2. Antioxidant activity of Fish Protein Hydrolysates from Sardinella longiceps

    OpenAIRE

    JEEVITHA K; MOHANA PRIYA K; SAMANTA S. KHORA

    2014-01-01

    Sardinella longiceps were hydrolysed with the different concentration of proteolytic enzymes trypsin to obtain peptides with antioxidant activity. The degree of hydrolysis and yield of hydrolysates were found increasing with increasing the concentration of enzyme. The antioxidant activities of hydrolysates were investigated through various assays. The hydrolysate exhibited the higher reducing power capability. The hydrolysates has shown a higher scavenging activity against DPPH, Superoxide, h...

  3. Aurelia aurita bio-inspired tilt sensor

    Science.gov (United States)

    Smith, Colin; Villanueva, Alex; Priya, Shashank

    2012-10-01

    The quickly expanding field of mobile robots, unmanned underwater vehicles, and micro-air vehicles urgently needs a cheap and effective means for measuring vehicle inclination. Commonly, tilt or inclination has been mathematically derived from accelerometers; however, there is inherent error in any indirect measurement. This paper reports a bio-inspired tilt sensor that mimics the natural balance organ of jellyfish, called the ‘statocyst’. Biological statocysts from the species Aurelia aurita were characterized by scanning electron microscopy to investigate the morphology and size of the natural sensor. An artificial tilt sensor was then developed by using printed electronics that incorporates a novel voltage divider concept in conjunction with small surface mount devices. This sensor was found to have minimum sensitivity of 4.21° with a standard deviation of 1.77°. These results open the possibility of developing elegant tilt sensor architecture for both air and water based platforms.

  4. Extraction of fish body oil from Sardinella longiceps by employing direct steaming method and its quantitative and qualitative assessment

    Directory of Open Access Journals (Sweden)

    Moorthy Pravinkumar

    2015-12-01

    Full Text Available Objective: To analyze the quantitative and qualitative properties of the extracted fish oil from Sardinella longiceps (S. longiceps. Methods: Four size groups of S. longiceps were examined for the extraction of fish oil based on length. The size groups included Group I (size range of 7.1–10.0 cm, Group II (size range of 10.1–13.0 cm, Group III (size range of 13.1–16.0 cm and Group IV (size range of 16.1– 19.0 cm. Fish oil was extracted from the tissues of S. longiceps by direct steaming method. The oil was then subjected to the determination of specific gravity, refractive index, moisture content, free fatty acids, iodine value, peroxide value, saponification value and observation of colour. Results: The four groups showed different yield of fish oil that Group IV recorded the highest values of (165.00 ± 1.00 mL/kg followed by Group III [(145.66 ± 1.15 mL/kg] and Group II [(129.33 ± 0.58 mL/kg], whereas Group I recorded the lowest values of (78.33 ± 0.58 mL/ kg in monsoon season, and the average yield was (180.0 ± 4.9 mL/kg fish tissues. These analytical values of the crude oil were well within the acceptable standard values for both fresh and stocked samples. Conclusions: The information generated in the present study pertaining to the quantitative and qualitative analysis of fish oil will serve as a reference baseline for entrepreneurs and industrialists in future for the successful commercial production of fish oil by employing oil sardines.

  5. Extraction of fish body oil fromSardinella longiceps by employing direct steaming method and its quantitative and qualitative assessment

    Institute of Scientific and Technical Information of China (English)

    Moorthy Pravinkumar; Lawrence Xavier Eugien; Chinnathambi Viswanathan; Sirajudeen Mohammad Raffi

    2015-01-01

    Objective:To analyze the quantitative and qualitative properties of the extracted fish oil from Sardinella longiceps(S. longiceps). Methods: Four size groups ofS. longiceps were examined for the extraction of fish oil based on length. The size groups included Group I (size range of 7.1–10.0 cm), Group II (size range of 10.1–13.0 cm), GroupIII (size range of 13.1–16.0 cm) and Group IV (size range of 16.1–19.0 cm). Fish oil was extracted from the tissues ofS. longiceps by direct steaming method. The oil was then subjected to the determination of specific gravity, refractive index, moisture content, free fatty acids, iodine value, peroxide value, saponification value and observation of colour. Results: The four groups showed different yield of fish oil that Group IV recorded the highest values of (165.00 ± 1.00) mL/kg followed by GroupIII [(145.66 ± 1.15) mL/kg] and Group II [(129.33 ± 0.58) mL/kg], whereas Group I recorded the lowest values of (78.33 ± 0.58) mL/kg in monsoon season, and the average yield was (180.0 ± 4.9) mL/kg fish tissues. These analytical values of the crude oil were well within the acceptable standard values for both fresh and stocked samples. Conclusions:The information generated in the present study pertaining to the quantitative and qualitative analysis of fish oil will serve as a reference baseline for entrepreneurs and industrialists in future for the successful commercial production of fish oil by employing oil sardines.

  6. Genetic Differentiation of Japanese Sardinella (Sardinella zunasi)Populations in the Northwest Pacific Revealed by ISSR Analysis

    Institute of Scientific and Technical Information of China (English)

    YING Yiping; GAO Tianxiang; MIAO Zhenqing

    2011-01-01

    Knowledge of population genetic structure plays an important role in fisheries management.In this research,Inter-Simple-Sequence-Repeat (ISSR) markers were employed to evaluate the genetic structure of Japanese sardinella (Sardinella zunasi) populations in the Northwest Pacific.Eighty seven individuals from 5 locations were screened using 4 highly polymorphic primers.A total of 173 polymorphic loci were detected out of 191 loci amplified.Small but significant genetic differentiation was detected between the Chinese and Japanese populations by both AMOVA and pairwise Fsr analyses,which was further supported by cluster analysis.We consider that climate change during glaciations should be responsible for the genetic differentiation.Isolation by geographic distance among populations was observed,indicating that the distance might also lead to the genetic differentiation.However,no genetic structure was found within the populations off both the Chinese and Japanese coasts,indicating a high-level along-coast gene flow,which might result from ocean current transport and common ground for over-wintering.

  7. [Differential gene expression in the jellyfish Aurelia aurita].

    Science.gov (United States)

    Matveev, I V

    2005-01-01

    The body of Aurelia aurita, as well as other diploblasts, consists of two epithelial layers: ectodermal and gastral epithelium. These two tissues are separated by mesoglea, or extracellular matrix. In most coelenterates mesoglea is acellular. In A. aurita mesogleal cells are scattered in mesoglea. Differential display PCR was used to compare mRNA pools from ectodermal epithelium, gastral epithelium and mesoglea. 4 novel gene fragments were cloned and sequenced. According to RTPCR results, one of these fragments is differentially expressed in the ectodermal epithelium.

  8. Sardinella brasiliensis (Steindachner, 1879 (Teleostei: Clupeidae, nome válido aplicado à sardinha-verdadeira no sudeste do Brasil

    Directory of Open Access Journals (Sweden)

    José Lima de Figueiredo

    2010-01-01

    Full Text Available A história nomenclatural de Sardinella brasiliensis (Steindachner, 1879 e de seu nome de substituição, Sardinella janeiro (Eigenmann, 1894 é apresentada, sendo confirmada a validade do primeiro por meio da aplicação dos dispositivos do Código Internacional de Nomenclatura Zoológica.The nomenclatural history of Sardinella brasiliensis (Steindachner, 1879 and its replacement name Sardinella janeiro (Eigenmann, 1894 is presented, and the validity of the first confirmed through the application of the provisions of the International Code of Zoological Nomenclature.

  9. Feeding and ecomorphology of three clupeoids in the N Aegean Sea

    Directory of Open Access Journals (Sweden)

    P. K. KARACHLE

    2013-12-01

    Full Text Available The present study examines the feeding habits of anchovy (Engraulis encrasicolus, sardine (Sardina pilchardus and round sardinella (Sardinella aurita. The results are combined with previously published information on feeding-related morphological features (i.e. mouth area, intestine length and tail area in order to explore morphological affinities between species and the effect of ecomorphology on their co-existence. These species were mainly zooplanktivorous and no dietary differences were found with sex and season. Anchovy preyed mainly on Crustacea larvae, whereas sardine and round sardinella on Copepoda. In the majority of cases (>90%, the individual fractional trophic level of all species ranged between 3.0 and 3.5, classifying them as omnivores with preference to animals. The feeding-related morphological features differed between anchovy and the two other species, whereas only intestine length differed between sardine and round sardinella. The fact that round sardinella’s diet and morphology show a greater resemblance to those of sardine, further support the hypothesis that is a particulate feeder as sardine. Hence the three species tend to exploit the same food resources differently throughout the year. Thus, they make best use of the environment and its resources, in order to avoid competition and achieve optimum feeding conditions throughout their life cycles

  10. Feeding rates of the jellyfish Aurelia aurita on fish larvae

    DEFF Research Database (Denmark)

    Titelman, J.; Hansson, L.J.

    2006-01-01

    of prey captured by A. aurita during 2.5 h of feeding at extremely high prey concentration (> 200 prey l(-1)). Clearance rate in darkness scaled with jellyfish diameter to a power of similar to 1.7 for jellyfish 3.9-9.5 cm in diameter. The jellyfish did not alter their umbrella pulse frequency in response...

  11. Furan in canned sardines and other fish.

    Science.gov (United States)

    Pye, Celine; Crews, Colin

    2014-01-01

    Thirty-seven different samples of canned sardines and other fish sold in the United Kingdom were analysed for their furan content using a validated automated headspace gas chromatography-mass spectrometry procedure. All 37 samples contained detectable furan, with an average level of 26 μg kg(-1). The maximum furan content was in canned fish containing tomato sauce, which had an average of 49 μg kg(-1) and in canned fish packed with lemon which had an average of 55 μg kg(-1). All fish in brine or in oil contained less than 20 μg kg(-1) furan. Furan levels recorded in fish packed in extra virgin olive oil were low with an average of 2 μg kg(-1).

  12. Aurelia aurita Ephyrae Reshape a Coastal Microbial Community

    Science.gov (United States)

    Zoccarato, Luca; Celussi, Mauro; Pallavicini, Alberto; Fonda Umani, Serena

    2016-01-01

    Over the last two decades, increasing attention has been paid to the impact of jellyfish blooms on marine communities. Aurelia aurita is one of the most studied of the Scyphozoans, and several studies have been carried out to describe its role as a top-down controller within the classical food web. However, little data are available to define the effects of these jellyfish on microbial communities. The aims of this study were to describe the predation impact of A. aurita ephyrae on a natural microplanktonic assemblage, and to determine any reshaping effects on the prokaryote community composition and functioning. Surface coastal water was used to set up a 24-h grazing experiment in microcosms. Samples were collected to determine the variations in prey biomass, heterotrophic carbon production (HCP), extracellular leucine aminopeptidase activity, and grazing pressure. A next-generation sequencing technique was used to investigate biodiversity shifts within the prokaryote and protist communities through the small subunit rRNA tag approach. This study shows that A. aurita ephyrae were responsible for large decreases in the abundances of the more motile microplankton groups, such as tintinnids, Dinophyceae, and aloricate ciliates. Bacillariophyceae and Mediophyceae showed smaller reductions. No evidence of selective predation emerged in the analysis of the community diversity down to the family level. The heterotrophic prokaryote biomass increased significantly (by up to 45%), in parallel with increases in HCP and leucine aminopeptidase activity (40%). Significant modifications were detected in prokaryotic community composition. Some classes of Gammaproteobacteria and Flavobacteriia showed higher relative abundances when exposed to A. aurita ephyrae, while there was a net decrease for Alphaproteobacteria. Overall, this study provides new insight into the effects of A. aurita on microbial communities, underlining their selective predation toward the more motile groups of

  13. Aurelia aurita Ephyrae Reshape a Coastal Microbial Community.

    Science.gov (United States)

    Zoccarato, Luca; Celussi, Mauro; Pallavicini, Alberto; Fonda Umani, Serena

    2016-01-01

    Over the last two decades, increasing attention has been paid to the impact of jellyfish blooms on marine communities. Aurelia aurita is one of the most studied of the Scyphozoans, and several studies have been carried out to describe its role as a top-down controller within the classical food web. However, little data are available to define the effects of these jellyfish on microbial communities. The aims of this study were to describe the predation impact of A. aurita ephyrae on a natural microplanktonic assemblage, and to determine any reshaping effects on the prokaryote community composition and functioning. Surface coastal water was used to set up a 24-h grazing experiment in microcosms. Samples were collected to determine the variations in prey biomass, heterotrophic carbon production (HCP), extracellular leucine aminopeptidase activity, and grazing pressure. A next-generation sequencing technique was used to investigate biodiversity shifts within the prokaryote and protist communities through the small subunit rRNA tag approach. This study shows that A. aurita ephyrae were responsible for large decreases in the abundances of the more motile microplankton groups, such as tintinnids, Dinophyceae, and aloricate ciliates. Bacillariophyceae and Mediophyceae showed smaller reductions. No evidence of selective predation emerged in the analysis of the community diversity down to the family level. The heterotrophic prokaryote biomass increased significantly (by up to 45%), in parallel with increases in HCP and leucine aminopeptidase activity (40%). Significant modifications were detected in prokaryotic community composition. Some classes of Gammaproteobacteria and Flavobacteriia showed higher relative abundances when exposed to A. aurita ephyrae, while there was a net decrease for Alphaproteobacteria. Overall, this study provides new insight into the effects of A. aurita on microbial communities, underlining their selective predation toward the more motile groups of

  14. Although South African sardine (or pilchard, Sardinops sagax) were ...

    African Journals Online (AJOL)

    spamer

    (iii) as anchovy and sardine frequently shoal together during their first ... Fishery Resources Division, Food and Agricultural Organization, Via delle Terme di Caracalla 00100, Rome, Italy. ...... risk and “scope for growth” as performance criteria.

  15. Anchovy Engraulis capensis and sardine Sardinops sagax currently ...

    African Journals Online (AJOL)

    spamer

    within-season variability in factors affecting sardine Sardinops sagax and anchovy Engraulis capensis recruitment. .... variogram. ... formerly SF, unpublished data) were based on length ..... the front offshore, along with many eggs and larvae.

  16. Anti-inflammation effects of Sardinella longicep oil against paw oedema on Rattus novergicus induced by 1% carrageenan

    Directory of Open Access Journals (Sweden)

    Rima Parwati Sari

    2010-09-01

    Full Text Available Background: People usually used non steroid anti-inflammation drugs (NSAID such as aspirin in chronic inflammation treatment. However, using NSAID at long term therapy will cause many effects such as nausea and vomiting. Sardinella longiceps oil, on the other side, is reported as an alternative treatment for anti-inflammation since it is natural and also contained eicosapentaenoid acid (EPA and decohexaenoic acid (DHA. Thus, it may reduce paw oedema. Purpose: The aim of this study was to know anti-inflammation effects of Sardinella longiceps oil against paw oedema of Wistar rats induced by 1% carrageenan. Methods: The samples of this research were 32 Wistar rats which were divided into four groups, in group 1, the rats were given aquadest; in group 2, the rats were given aspirin; in group 3, the rats were given 1 ml Sardinella longiceps oil; in group 4, the rats were given 1.5 ml Sardinella longiceps oil. All of the rats, nevertheless, were given intraplantar induction of 1% carrageenan into the paw of rats to induce the inflammation condition. Results: All data were tested with normality test. The normal data were then analyzed with Homogenity of Variances and also ANOVA test which result showed significant differences. The data which showed significant differences were tested again with LSD test. result then showed that group given 1 ml Sardinella longiceps oil and group given 1.5 ml Sardinella longiceps oil had no significant differences from group given aspirin, but there were significant differences between group given 1 ml Sardinella longiceps oil and group given 1.5 ml Sardinella longiceps oil, and also between group given 1.5 ml Sardinella longiceps oil and group given aquadest. Conclusion: Sardinella longiceps oil could reduce paw oedema in Wistar rats induced with 1% carrageenan.Latar belakang: Penggunaan obat anti-inflamasi non steroid (OAINS seperti aspirin sering digunakan dalam pengobatan inflamasi kronis. Namun penggunaan OAINS

  17. Sphingophosphonolipids, phospholipids, and fatty acids from Aegean jellyfish Aurelia aurita.

    Science.gov (United States)

    Kariotoglou, D M; Mastronicolis, S K

    2001-11-01

    The goal of this study is to elucidate and identify several sphingophosphonolipids from Aurelia aurita, an abundant but harmless Aegean jellyfish, in which they have not previously been described. Total lipids of A. aurita were 0.031-0.036% of fresh tissue, and the lipid phosphorus content was 1.3-1.7% of total lipids. Phosphonolipids were 21.7% of phospholipids and consisted of a major ceramide aminoethylphosphonate (CAEP-I; 18.3%), as well as three minor CAEP (II, III, IV) methyl analogs at 1.3, 1.1, and 1.0%, respectively. The remaining phospholipid composition was: phosphatidylcholine, 44.5%, including 36.2% glycerylethers; phosphatidylethanolamine, 18.6%, including 4.5% glycerylethers; cardiolipin, 5.6%; phosphatidylinositol, 2.6%; and lysophosphatidylcholine, 5.0%. In CAEP-I, saturated fatty acids of 14-18 carbon chain length were 70.8% and were combined with 57.3% dihydroxy bases and 23.4% trihydroxy bases. The suite of the three minor CAEP methyl analogs were of the same lipid class based on the head group, but they separated into three different components because of their polarity as follows: CAEP-II and CAEP-III differentiation from the major CAEP-I was mainly due to the increased fatty acid unsaturation and not to a different long-chain base, but the CAEP-IV differentiation from CAEP-I, apart from fatty acid unsaturation, was due to the increased content of hydroxyl groups originated from both hydroxy fatty acids and trihydroxy long-chain bases. Saturated fatty acids were predominant in total (76.7%), polar (83.0%), and neutral lipids (67.6%) of A. aurita. The major phospholipid components of A. aurita were comparable to those previously found in a related organism (Pelagia noctiluca), which can injure humans.

  18. Aurelia aurita-Cnidarian with a prominent medusiod stage.

    Science.gov (United States)

    Matveev, Ivan V; Adonin, Leonid S; Shaposhnikova, Tatiana G; Podgornaya, Olga I

    2012-01-15

    Aurelia aurita has a complex life cycle that consists of several stages including alternating generations of medusa and polyps, huge sexual, and tiny asexual stages. Cnidarian is thought to possess two tissue layers: endoderm (gastroderm) and ectoderm, which are separated by mesoglea in medusa. The determination of the composition of the A. aurita jellyfish mesoglea was performed. New protein "mesoglein" was determined as one of the main components of mesoglea. Mesoglein is synthesized by mesogleal cells (Mc), which are populated A. aurita mesoglea as a high molecular mass precursor. Mc are involved in the formation of noncollagenous "elastic" fibers. Deduced amino acid sequence of mesoglein contains Zona Pellucida (ZP) domain and Delta/Serrate/Lag-2 domain. According to reverse transcription PCR, mesoglein is expressed in the mature medusa exclusively in the Mc. The sperm binding to the ZP is particularly important for successful fertilization. Antibodies against mesoglein stain the plate in the place of contact of germinal epithelium and oocyte. The structure found was named the "contact plate." The contact plate could be the precursor of the ZP. All our data suggest that Mc and, probably, the whole mesoglea originate from the epidermis (ectoderm). Computer search for mesoglein relatives reveals Nematostella and Trichoplax proteins as predicted ORFs, indicating that ZP proteins are quite ancient purchase in the evolution. © 2011 Wiley Periodicals, Inc.

  19. Aurelia aurita (Cnidaria) oocytes' contact plate structure and development.

    Science.gov (United States)

    Adonin, Leonid S; Shaposhnikova, Tatyana G; Podgornaya, Olga

    2012-01-01

    One of the A. aurita medusa main mesoglea polypeptides, mesoglein, has been described previously. Mesoglein belongs to ZP-domain protein family and therefore we focused on A.aurita oogenesis. Antibodies against mesoglein (AB RA47) stain the plate in the place where germinal epithelium contacts oocyte on the paraffin sections. According to its position, we named the structure found the "contact plate". Our main instrument was AB against mesoglein. ZP-domain occupies about half of the whole amino acid sequence of the mesoglein. Immunoblot after SDS-PAGE and AU-PAGE reveals two charged and high M(r) bands among the female gonad germinal epithelium polypeptides. One of the gonads' polypeptides M(r) corresponds to that of mesogleal cells, the other ones' M(r) is higher. The morphological description of contact plate formation is the subject of the current work. Two types of AB RA47 positive granules were observed during progressive oogenesis stages. Granules form the contact plate in mature oocyte. Contact plate of A.aurita oocyte marks its animal pole and resembles Zona Pellucida by the following features: (1) it attracts spermatozoids; (2) the material of the contact plate is synthesized by oocyte and stored in granules; (3) these granules and the contact plate itself contain ZP domain protein(s); (4) contact plate is an extracellular structure made up of fiber bundles similar to those of conventional Zona Pellucida.

  20. SaRDIn - A Safe Reconfigurable Distributed Interlocking

    DEFF Research Database (Denmark)

    Fantechi, A.; Gnesi, S.; Haxthausen, Anne Elisabeth;

    2016-01-01

    Current computer-based interlocking systems most often have a centralized design, with all logic residing in a single computer. Centralized interlockings are complex to design. Following the general trend in Cyber-Physical Systems, the SaRDIn (Safe Reconfigurable Distributed Interlockings) concept...... promises a radically simpler solution. The novelty of the SaRDIn concept is a fine-grained distribution of the logic over all processors deployed at the sensors and actuators along the track layout, i.e. within the track circuits, point machines, signals, etc. The advantages and drawbacks...

  1. Modelling physical-biological interactions in the Southeast Brazil Bight: transport patterns of Brazilian Sardine larvae

    Science.gov (United States)

    Faggiani Dias, D.; Gherardi, D. F.; Pezzi, L. P.

    2013-05-01

    The advection of Brazilian Sardine (Sardinella brasiliensis) eggs and larvae in the SBB was modeled using an individual-based model (Ichthyop) and a hydrodynamic model (Regional Ocean Modeling System, ROMS) to test for differences in larval retention for five spawning areas with high probability of egg occurrence: i) two areas north of the domain - Cape Frio and Rio de Janeiro, ii) one in the middle in Sao Sebastiao, and iii) two in the South in Paranagua. According to previous studies, this encompasses the known spawning habitat. Advective processes and physical characteristics, such as water temperature and salinity, were considered to determine larvae transport and survival. The hydrodynamic model grid has a horizontal resolution of 1/12o. Results of monthly mean Sea Surface Temperature (MSST) and Sea Surface Height (MSSH) indicate there isn't warming or cooling trend over the years, and the seasonal cycle well represented. These results were compared with satellite-derived data from the AVHRR sensor and AVISO project. Model results accurately represent the position and shape of the main surface structures observed in the satellite data. Monthly MSST maps for the experiment period indicate that the model tends to underestimate temperatures in upwelling areas and overestimate in the Brazil Current region, with differences mostly around ±1oC. For MSSH, although the model represents well the main surface ocean structures, it tends to underestimate along the domain. Temperature-salinity diagrams plotted in a coastal area for December of four years (1985, 1986, 1987 and 1988), near Ubatuba region, are consistent with field collected data, suggesting that the main water masses in SBB are reliably represented. The IBM experiments were carried out during the summer of six years (1980, 1981, 1988, 1991, 1992 and 1993). For each year, 20000 eggs were released, distributed in the five areas, and tracked for 45 days. At the end of simulation, the mortality due to

  2. Worldwide, where anchovies Engraulis spp. and sardine Sardinops ...

    African Journals Online (AJOL)

    spamer

    sardine Sardinops sagax co-occur, the two species alternate in ... the west coasts of South America and southern Africa, ... of extinction in the wild within 100 years (Croxall et al. 1996) ..... able to penguins year-round, compared to the seasonal.

  3. Extraction of high added value biological compounds from sardine, sardine-type fish and mackerel canning residues--a review.

    Science.gov (United States)

    Ferraro, Vincenza; Carvalho, Ana P; Piccirillo, Clara; Santos, Manuela M; Castro, Paula M L; Pintado, Manuela E

    2013-08-01

    Different valuable compounds, which can be employed in medicine or in other industries (i.e. food, agrochemical, pharmaceutical) can be recovered from by-products and waste from the fish canning industries. They include lipids, proteins, bio-polymers, minerals, amino acids and enzymes; they can be extracted from wastewaters and/or from solid residues (head, viscera, skin, tails and flesh) generated along the canning process, through the filleting, cooking, salting or smoking stages. In this review, the opportunities for the extraction and the valorisation of bioactive compounds from sardine, sardine-type fish and mackerel canning residues are examined and discussed. These are amongst the most consumed fishes in the Mediterranean area; moreover, canning is one of the most important and common methods of preservation. The large quantities of by-products generated have great potentials for the extraction of biologically desirable high added value compounds.

  4. Anticoagulant activity of Moon jellyfish (Aurelia aurita) tentacle extract.

    Science.gov (United States)

    Rastogi, Akriti; Biswas, Sumit; Sarkar, Angshuman; Chakrabarty, Dibakar

    2012-10-01

    Moon jellyfish (Aurelia aurita) tentacle extract was studied for its anticoagulant activity in vitro. The Jellyfish Tentacle Extract (JFTE) showed very strong fibrinogenolytic activity by cleaving Aα and Bβ chain of fibrinogen molecule. The fibrinogenolytic activity was found to be stronger than some snake venom derived anticoagulants. JFTE also completely liquefied fibrin clots in 24 h. JFTE was found to contain both high and low molecular weight proteins/peptides. The fibrinogenolysis appears to be caused by high molecular weight fractions of the extract. It has been also noted that PMSF significantly reduced fibrinogenolytic activity and heating totally abolished it. Autolytic degradation of the high molecular weight protein was also noted. Autolysis slowed down, but did not abolish the fibrinogenolytic activity of the extract. Copyright © 2012 Elsevier Ltd. All rights reserved.

  5. Regulation of polyp-to-jellyfish transition in Aurelia aurita.

    Science.gov (United States)

    Fuchs, Björn; Wang, Wei; Graspeuntner, Simon; Li, Yizhu; Insua, Santiago; Herbst, Eva-Maria; Dirksen, Philipp; Böhm, Anna-Marei; Hemmrich, Georg; Sommer, Felix; Domazet-Lošo, Tomislav; Klostermeier, Ulrich C; Anton-Erxleben, Friederike; Rosenstiel, Philip; Bosch, Thomas C G; Khalturin, Konstantin

    2014-02-03

    The life cycle of scyphozoan cnidarians alternates between sessile asexual polyps and pelagic medusa. Transition from one life form to another is triggered by environmental signals, but the molecular cascades involved in the drastic morphological and physiological changes remain unknown. We show in the moon jelly Aurelia aurita that the molecular machinery controlling transition of the sessile polyp into a free-swimming jellyfish consists of two parts. One is conserved and relies on retinoic acid signaling. The second, novel part is based on secreted proteins that are strongly upregulated prior to metamorphosis in response to the seasonal temperature changes. One of these proteins functions as a temperature-sensitive "timer" and encodes the precursor of the strobilation hormone of Aurelia. Our findings uncover the molecule framework controlling the polyp-to-jellyfish transition in a basal metazoan and provide insights into the evolution of complex life cycles in the animal kingdom. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. Effect of temperature, anaerobiosis, stirring and salt addition on natural fermentation silage of sardine and sardine wastes in sugarcane molasses.

    Science.gov (United States)

    Zahar, M; Benkerroum, N; Guerouali, A; Laraki, Y; El Yakoubi, K

    2002-04-01

    Conditions for a natural fermentation during ensilage of sardines or their waste in sugarcane molasses (60:40 w/w) were evaluated regarding the effect of temperature (15, 25 and 35 degrees C), anaerobiosis (closed vs. open jars), daily stirring of the mixture, and salt addition to the initial mix at 5% (w/w) level. Successful natural fermentation took place in sardine silages incubated at 25 or 35 degrees C in open jars to reach a pH of 4.4 in about 2 and 1 weeks, respectively. For samples kept at 15 degrees C, the pH decline was very slow and pH did not decrease below 5.5 after one month of incubation. At 25 degrees C, the most favorable conditions for silage of sardine waste in cane molasses, as evidenced by the fastest decline in pH to a stable value of about 4.4, were achieved in closed jars and with daily stirring of the mix. The pH 4.4 was reached in one week with an advance of at least 3 days compared to the other conditions (open jars and closed jars without daily stirring). Addition of salt at 5% (w/w) in the mix before incubation inhibited the fermentation process.

  7. Changes on quality parameters of surimi and surimi gels produced from frozen whiting and sardine

    Directory of Open Access Journals (Sweden)

    Evren Burcu Şen

    2017-03-01

    Full Text Available In this study, whiting (Theragra chalcogramma, Pallas, 1814 and sardine (Sardina pilchardus, Walbum, 1792 were used as a raw material for surimi production. Every 30 days direct gels and kamaboko gels produced from frozen surimi packages during the 60 days storage. At the end of the washing sycle of the fish mince 88% of myoglobin content in sardine mince were suspended. Protein amount of whiting, whiting surimi, sardine and sardine surimi were detected 19.65 %, 18.00 %, 20.96 % and 19.27 %, respectively. Whitening values of surimi gels from whiting and sardine surimi were detected higher in direct gels at 60. days of storage as 63.57 and 67.62 respectively. TBA values of whiting surimi was determined 2.71 mg malonaldehyde/kg at the end of 60 days storage while it was determined as 5.79 mg malonaldehyde/kg for sardine. According to the results of TBA and microbiological analyses, the surimi from sardine and whiting was not exceed the acceptable limits. Although surimi made from whiting were determined as ‘acceptable’ at second month of storage, surimi made from sardine were no longer acceptable because of the oxidation problem according to the results of sensory analyses.

  8. Optimization of a chemical method for skinning of sardines (Sardina pilchardus during canning processing

    Directory of Open Access Journals (Sweden)

    Manuela Vaz Velho

    2014-06-01

    Full Text Available Most of sardine (Sardina pilchardus catches is used for canning purposes. The most common product presentation is a beheaded sardine with skin and bones packed in a tin can. Canned sardines can also be presented skinless and boneless. For this last type of product, after beheading and evisceration, sardines are placed in trays, cooked and then skinned by hand, one by one, and placed in the tins, a process involving high labour costs. The aim of this work was to develop a chemical process for peeling raw sardines and its subsequent application in a canning industry processing line just after the beheading and evisceration step and before cooking. Potassium hydroxide treatments (pellets a.r. 85% KOH were applied at concentrations of 2, 3 and 4% (v/v, distilled water. Frozen sardines were beheaded and eviscerated after thawing and immersed in the different potassium hydroxide solutions at 93ºC (pH respectively 13, 13 and 13.02 for 3 min and further washed with distilled water at 100°C. In this first set of experiments, fat sardines were used (average of 9.86% of fat, w/w. The best performance, with respect to skin removal, was achieved with the 2% potassium hydroxide immersion (pH 13. With this treatment the skin was totally removed after immersion. With the other tested concentrations portions of skin were always visible and in some cases changes in texture with breakdown of muscle structure and changes of colour occurred. It was decided to perform a second set of experiments using the 2% KOH treatment, but this time applied to low fat sardines (average of 4.77% of fat, w/w, following the same subsequent procedures. The results showed that the lower fat sardines are more prone to surface changes of colour and major muscle breaks than fat sardines after the potassium hydroxide treatment. In the canning industry for this type of product (skinless and boneless only fat sardines are used to assure the total removal of skin. This treatment of 2% KOH

  9. Population succession and feeding of scyphomedusae, Aurelia aurita, in a eutrophic tropical lagoon in Taiwan

    Science.gov (United States)

    Lo, W. T.; Chen, I. L.

    2008-01-01

    Seasonal dynamics and feeding of scyphomedusae, Aurelia aurita, were investigated monthly from 1999 to 2002 in relation to environmental conditions in Tapong Bay, a eutrophic tropical lagoon in southwestern Taiwan. Medusae appeared throughout the year but exhibited seasonal dynamics that were correlated with hydrographic features in the bay. Most ephyrae of A. aurita occurred mainly in the lower flushed and eutrophic inner bay, and during the cold, dry season between November and February. They grew to young medusae with a maximum abundance in spring (March-May), but their numbers abruptly decreased during the warm and rainy summer season in June-September. The remaining medusae then grew rapidly to a maximal size of 29 cm. Mature females spawned in the following autumn when precipitation decreased but zooplankton food was still abundant. These mature individuals decreased in size after spawning and in winter. Gut content analysis revealed that A. aurita fed mainly on copepods and copepod nauplii and less on bivalve larvae and fish eggs. Prey selectivity indices indicated that larger medusae selected positively for copepods while small size medusae preferred copepod nauplii. The overall feeding effect of A. aurita on the standing stock of zooplankton was significant (27%) in the bay. Our results suggest that tidal flow and dense oyster culture pens were the two most important factors influencing the spatial distribution pattern of A. aurita in the bay, while precipitation affected the population abundance seasonally; decreasing water temperature coincided with the mass release of ephyra in late autumn and winter.

  10. STATUS STOK SUMBERDAYA IKAN LEMURU (Sardinella lemuru Bleeker, 1853 DI PERAIRAN SELAT BALI

    Directory of Open Access Journals (Sweden)

    Arief Wujdi

    2015-12-01

    Full Text Available Ikan lemuru (Sardinella lemuru Bleeker, 1853 berperan penting baik secara ekologi ekosistem laut dan ekonomi masyarakat pesisir di Selat Bali. Tingginya permintaan pasar terhadap ikan lemuru mengakibatkan aktivitas penangkapannya dilakukan secara terus-menerus sepanjang tahun sehingga mengancam kelestariannya. Untuk mengelola sumber daya ikan lemuru ini tetap lestari, maka salah satu bahan masukan yang diperlukan adalah status stok terkini. Namun, ketersediaan data yang terbatas menjadi penghambat dalam upaya mengevaluasi status pemanfaatan stok, khususnya untuk kegiatan perikanan skala kecil. Penelitian ini bertujuan untuk mengetahui status stok sumberdaya ikan lemuru di perairan Selat Bali yang dilakukan dari Agustus 2010 hingga Desember 2011. Pengkajian stok ikan lemuru dilakukan dengan pendekatan ratio potensi pemijahan yang berbasis ukuran panjang. Studi pustaka juga dilakukan untuk mendapatkan parameter-parameter life history sebagai pendukung analisis. Hasil penelitian menunjukkan bahwa status stok ikan lemuru telah mengalami eksploitasi berlebih (over exploited yang diindikasikan dengan nilai Spawning Potential Ratio (SPRM. Upaya pemulihan stok perlu dilakukan sebagaimana dijelaskan dalam penelitian ini agar tercapainya SPR menjadi 20% dan 40% sebagai batas dan target biologi untuk keberlanjutan sumberdaya ikan lemuru. Bali sardinella (Sardinella lemuru Bleeker, 1853 plays an important role in terms of the ecology of marine ecosystems and also the economy and livelihoods of coastal communities in Bali Strait. High market demand has consequence in fishing activities, which is carried out continuously throughout the year, so that could threaten of resources sustainability. So that current stock status was needed as important input for management measures. However, evaluation of stock status is constrained by lack of the data, especially for small-scale fisheries. This study aims to determine the stock status of Bali sardinella in the Bali

  11. [On some features of embryonic development and metamorphosis of Aurelia aurita (Cindaria, Scyphozoa)].

    Science.gov (United States)

    Maĭorova, T D; Kosevich, I A; Melekhova, O P

    2012-01-01

    Aurelia aurita is a cosmopolite species of scyphomedusae. Its major structural patterns and life cycle are well investigated. This work provides a detailed study on development and structure of the planula in A. aurita until it completes its metamorphosis. Lifetime observations and histological study performed during the settlement and metamorphosis of the planulae demonstrated that the inner manibrium linen of primary polyp (gastroderm) develops from the ectoderm of the planula posterior end. The spatial and temporal dynamics of serotonergic cells from the early embryonic stages until the formation of the primary polyp were studied for the first time. In addition, the distribution oftyrosinated tubulin and neuropeptide RFamide at different stages ofA. aurita development were studied.

  12. On natural metamorphosis inducers of the cnidarians Hydractinia echinata (Hydrozoa) and Aurelia aurita (Scyphozoa)

    Science.gov (United States)

    Kroiher, M.; Berking, S.

    1999-11-01

    Hydractinia echinata and Aurelia aurita produce motile larvae which undergo metamorphosis to sessile polyps when induced by external cues. The polyps are found at restricted sites, A. aurita predominantly on rocks close to the shore, H. echinata on shells inhabited by hermit crabs. It has been argued that the differential distribution of the polyps in their natural environment largely reflects the distribution of the natural metamorphosis-inducing cues. In the case of H. echinata, bacteria of the genus Alteromonas were argued to meet these conditions. We found that almost all substrates collected in the littoral to induce metamorphosis in H. echinata, and several bacterial strains isolated from the sea, including the common E. coli, induce metamorphosis efficiently. In A. aurita metamorphosis may be induced by the water-air interface, whereby metamorphosis precedes (final) settlement.

  13. [Protein composition of mesoglea and mesogloeal cells of medusa Aurelia aurita].

    Science.gov (United States)

    Shaposhnikova, T G; Napara, T O; Podgornaia, O I

    2002-01-01

    Protein composition of mesoglea of the scyphomedusa Aurelia aurita was revealed in SDS-PAGE. Some major bands are visible in mesoglea of a mature medusa: 30, 45-47, 85 kDa, three bands between 100-200 kDa, and several bands with molecular weights > 300 kDa. Polyclonal antisera RA45/47 against protein 45 kDa were raised. RA45/47 react with 45-47 kDa protein in mesogleal sample and protein 120 kDa in mesogleal cells on immunoblot. Immunohistochemical analysis of A. aurita histological sections of young and mature medusae showed antigen localization in mesogleal cell granules and in the apical part of ectodermal cells. In mature medusae, the antigen was localized also in elastic fibers. We can conclude that in A. aurita mesogleal cells, along with ectodermal cells, take part in the formation of extracellular matrix of mesoglea.

  14. Chemische Zusammensetzung und Biomasseparameter der Ohrenqualle Aurelia aurita

    Science.gov (United States)

    Schneider, Gerald

    1988-06-01

    During summer 1982 and 1983, measurements on the chemical composition and biomass parameters of Aurelia aurita from Kiel Fjord, Western Baltic, were made. The relationships between size vs. wet weight and wet weight vs. carbon content were determined. Dry weight accounted for less than 2% of wet weight in both years. Carbon, nitrogen and phosphorus content of whole medusae were 51 52, 14 and 1.4 mg/g dry weight (dw) respectively. The C:N ratio (by weight) was about 3.7 and the C:N:P-ratio (by atoms) was determined to be 94:22:1. Proteins and carbohydrates made up 59 and 29 mg/g dw. Lipids were estimated to be 19 mg/g dw. Using these data, it was possible to calculate the caloric content of the medusae to be about 0.55 cal/mg dw. In summer 1982, the organic content of different tissues (gonad, oral arm, umbrella) in unfertilized and fertilized females was studied. In unfertilized females, the gonads exhibited the highest values, the umbrella had the lowest values and the values of oral arm tissue were intermediate. However, in fertilized females the oral arm tissue was enriched, whereas the gonads were impoverished; both types of tissues exhibited very similar values. This pattern can be explained by the transfer of fertilized eggs from the gonads into the brood pouches of the oral arms.

  15. Ulcerative umbrellar lesions in captive moon jelly (Aurelia aurita) medusae.

    Science.gov (United States)

    LaDouceur, E E B; Garner, M M; Wynne, J; Fish, S; Adams, L

    2013-05-01

    Over a period of 6 months, dozens of moon jelly (Aurelia aurita) medusae from a single-species exhibit at the California Science Center (CSC) developed exumbrellar ulcers. Ulcers were progressive, causing umbrellar creases that expanded radially to the bell rim and occasional adoral erosions that extended into gastrovascular cavities. Husbandry interventions, including addition of ultraviolet light sterilizers, repopulation with fresh cultures, and enclosure disinfection, did not arrest the recurrence of lesions. Biopsies or whole specimens representing 17 medusae (15 affected and 2 grossly unaffected) from CSC and 2 control medusae from Aquarium of the Pacific were submitted to a private diagnostic laboratory and processed for light and electron microscopy. Microscopic lesions were present in all CSC medusae and were not observed or negligible in control medusae. Lesions included ulceration, necrosis, and hyperplasia in all umbrellar layers, with most severe lesions in the exumbrella and amoebocyte infiltration in the underlying mesoglea. Special stains, electron microscopy, and fungal culture did not associate microorganisms with the lesions. Bacterial cultures from the CSC population consistently grew Shewanella and Vibrio spp, both of which were considered commensal. Trauma and environmental stress are proposed as possible causes for the ulcers.

  16. Sardine Fish Oil By Sentrifugation and Adsorbent for Emulsion

    Directory of Open Access Journals (Sweden)

    Kristina Haryati

    2017-04-01

    Full Text Available Sardine fish meal by-product contain eicosapentaenoic acid (EPA and docosahexaenoic (DHA and it can be made as emulsion. The purpose of this study were to determine the best fish oil emulsion by mixingthe oil phase (lecithin 3% and oil and water phase (carboxymethyl cellulose/CMC 2% and fruit juice and then stored until creaming, and the emulsion is analyzed their viscosity, pH, percent of stability and longseparation. Sardine oil is separated from the emulsion and tested oxidation parameters. The best emulsion was fish oil emulsion after refined without citric acid (RTS with viscosity (2470.31 cP, pH (5.64, percent of stability (56.14% and long separation (14 days. Primary and secondary oxidation parameters of RTS  were FFA (14.87%, PV (14.43 meq/kg, AV (32.57 meq KOH/g, AnV (17.3 meq/kg, and Totox (46.16 meq/kg.

  17. Resilience of Key Biological Parameters of the Senegalese Flat Sardinella to Overfishing and Climate Change.

    Science.gov (United States)

    Ba, Kamarel; Thiaw, Modou; Lazar, Najih; Sarr, Alassane; Brochier, Timothée; Ndiaye, Ismaïla; Faye, Alioune; Sadio, Oumar; Panfili, Jacques; Thiaw, Omar Thiom; Brehmer, Patrice

    2016-01-01

    The stock of the Senegalese flat sardinella, Sardinella maderensis, is highly exploited in Senegal, West Africa. Its growth and reproduction parameters are key biological indicators for improving fisheries management. This study reviewed these parameters using landing data from small-scale fisheries in Senegal and literature information dated back more than 25 years. Age was estimated using length-frequency data to calculate growth parameters and assess the growth performance index. With global climate change there has been an increase in the average sea surface temperature along the Senegalese coast but the length-weight parameters, sex ratio, size at first sexual maturity, period of reproduction and condition factor of S. maderensis have not changed significantly. The above parameters of S. maderensis have hardly changed, despite high exploitation and fluctuations in environmental conditions that affect the early development phases of small pelagic fish in West Africa. This lack of plasticity of the species regarding of the biological parameters studied should be considered when planning relevant fishery management plans.

  18. Concentrations of Heavy Metals in Sardinella gibbosa (Bleeker, 1849) from the Coast of Balochistan, Pakistan.

    Science.gov (United States)

    Ahmed, Quratulan; Benzer, Semra; Elahi, Naeema; Ali, Qadeer Mohammad

    2015-08-01

    The concentrations of cadmium, iron, lead, manganese, nickel, and zinc in muscle tissue samples taken from Goldstripe sardinella (Sardinella gibbosa Bleeker, 1849) caught off the coast of Balochistan, Pakistan, in 2009 were determined. The samples were analyzed using a Perkin Elmer AAnalyst 700 flame atomic absorption spectrophotometer. The mean Cd, Fe, Mn, Ni, Pb, and Zn concentrations in the muscle samples were 0.65±0.05 µg g(-1), 23.39±1.97 µg g(-1), 4.35±0.22 µg g(-1), 0.61±0.07 µg g(-1), 0.39±0.04 µg g(-1), and 6.59±0.33 µg g(-1), respectively. The Cd, Fe, Pb, and Zn concentrations did not exceed the regulatory limits, but the Mn concentrations were high enough to potentially pose health risks to humans consuming S. gibbosa. Therefore, the Mn concentrations in S. gibbosa from the Balochistan coast should be monitored regularly.

  19. Seasonal variations of heavy metal concentration in Sardinella sindensis (Day, 1878 from Balochistan coast

    Directory of Open Access Journals (Sweden)

    Quratulan Ahmed

    2015-05-01

    Full Text Available Objective: To determine Fe, Cu, Zn, Pb and Cd concentrations in muscle of sind sardinella [Sardinella sindensis (S. sindensis] from Balochistan coast, Pakistan. Methods: S. sindensis were collected from Balochistan coast, Pakistan, at autumn inter monsoon, north east monsoon, spring inter monsoon and south west monsoon during October 2005-September 2006. All samples were analyzed for Fe, Cu, Zn, Pb and Cd by AAnalyst 700 flame atomic absorption spectrophotometer. Results: Generally, S. sindensis caught from Balochistan coast showed the accumulation level of Fe [(16.88 ± 5.60 µg/g], Cu [(2.74 ± 1.89 µg/g], Cd [(0.29 ± 0.25 µg/g], Zn [(4.29 ± 2.31 µg/g] and Pb [(0.24 ± 0.24 µg/g] in the muscle tissues. Conclusions: The metal concentrations in fish from Balochistan coast observed in this study were lower than the limit values. Therefore, all metal accumulations in S. sindensis have to be monitored continuously, especially in this region.

  20. Seasonal variations of heavy metal concentration in Sardinella sindensis (Day, 1878) from Balochistan coast

    Institute of Scientific and Technical Information of China (English)

    QuratulanAhmed; Semra Benzer; Naeema Elahi

    2015-01-01

    Objective: To determine Fe, Cu, Zn, Pb and Cd concentrations in muscle of sind sardinella [Sardinella sindensis (S. sindensis)] from Balochistan coast, Pakistan. Methods: S. sindensis were collected from Balochistan coast, Pakistan, at autumn inter monsoon, north east monsoon, spring inter monsoon and south west monsoon during October 2005-September 2006. All samples were analyzed for Fe, Cu, Zn, Pb and Cd by AAnalyst 700 flame atomic absorption spectrophotometer. Results: Generally, S. sindensis caught from Balochistan coast showed the accumulation level of Fe [(16.88 ± 5.60) µg/g], Cu [(2.74 ± 1.89) µg/g], Cd [(0.29 ± 0.25) µg/g], Zn [(4.29 ± 2.31) µg/g] and Pb [(0.24 ± 0.24) µg/g] in the muscle tissues. Conclusions: The metal concentrations in fish from Balochistan coast observed in this study were lower than the limit values. Therefore, all metal accumulations in S. sindensis have to be monitored continuously, especially in this region.

  1. Reconstruction of trophic pathways between plankton and the North Iberian sardine (Sardina pilchardus using stable isotopes

    Directory of Open Access Journals (Sweden)

    Antonio Bode

    2004-03-01

    Full Text Available Feeding on phyto- and zooplankton by juvenile (< 1 year old and adult sardines (Sardina pilchardus was inferred from analyses of natural abundance of stable carbon and nitrogen isotopes in samples from the northwestern Iberian Peninsula (Spain collected at the beginning of the upwelling season and peak spawning period of sardine. Plankton samples were fractionated through nets of 20, 200, 500, 1000 and 2000 ?m mesh-size and the muscle protein of individual sardines was isolated before isotopic determinations. Up to six planktonic components and two sardine feeding types were identified from the modes in the frequency distributions of isotope abundance values. Also, the most probable pathways for carbon and nitrogen flows between compartments were analysed. The resulting food web revealed a relatively large degree of omnivory, both in plankton and sardine components, which confirms that complex trophic interactions could also occur in pelagic upwelling ecosystems. Young sardines had isotope abundance values clustered around a single mode in the frequency distribution, while adult sardines displayed two main modes. These modes are interpreted as representative of two extreme feeding types: one related to the individual capture of zooplankton prey and the other to unselective filter-feeding. Although both types of feeding could include micro- (20-200 ?m and mesozooplankton (200-2000 ?m prey, phytoplankton appears to be ingested mainly by filter-feeding. However, even adult sardines must be mainly zoophagous to achieve the observed isotopic abundance values, taking into account current assumptions on stable isotope enrichment through trophic levels. From the differences in the resulting pathways using either carbon or nitrogen isotopes, we interpreted that sardines acquire most of the protein nitrogen from zooplankton while a substantial fraction of their carbon would derive from phytoplankton. These interpretations agree with the information

  2. Catch, effort and sampling strategies in the highly variable sardine fisheries around East Java, Indonesia.

    NARCIS (Netherlands)

    Pet, J.S.; Densen, van W.L.T.; Machiels, M.A.M.; Sukkel, M.; Setyohady, D.; Tumuljadi, A.

    1997-01-01

    Temporal and spatial patterns in the fishery for Sardinella spp. around East Java, Indonesia, were studied in an attempt to develop an efficient catch and effort sampling strategy for this highly variable fishery. The inter-annual and monthly variation in catch, effort and catch per unit of effort (

  3. Catch, effort and sampling strategies in the highly variable sardine fisheries around East Java, Indonesia.

    NARCIS (Netherlands)

    Pet, J.S.; Densen, van W.L.T.; Machiels, M.A.M.; Sukkel, M.; Setyohady, D.; Tumuljadi, A.

    1997-01-01

    Temporal and spatial patterns in the fishery for Sardinella spp. around East Java, Indonesia, were studied in an attempt to develop an efficient catch and effort sampling strategy for this highly variable fishery. The inter-annual and monthly variation in catch, effort and catch per unit of effort

  4. Peningkatan Mutu Terasi Kaya Antioksidan dan Protein Berbahan Kedelai, Jagung, dan Ikan Sarden (Sardinella Lemuru) Terfermentasi

    OpenAIRE

    Cherlin

    2016-01-01

    Nowadays people tend to consume fermented shrimp or flavoring to improve the taste of the food without looking at nutritional side of the flavoring. To counter this problem, it needs to diversify foods by replacing raw materials shrimps with sardines fermented flour, tempeh flour, and corn flour. This research was aimed to produce the fermented shrimp which is high in nutrient content such as protein and antioxidants needed by the human body. This research had 5 steps starting from making ...

  5. Minyak ikan Lemuru (Sardinella longicep menurunkan apoptosis osteoblas pada tulang alveolaris tikus wistar (Fish oil of Lemuru (Sardinella longicep reduced the osteoblast apoptosis in wistar rat alveolar bone

    Directory of Open Access Journals (Sweden)

    Didin Erma Indahyani

    2013-12-01

    Full Text Available Background: Periodontal disease is caused by periodontopatogen bacteria resulting the alveolar bone damage. The decrease of osteoblasts and the increased of osteoclasts can cause bone destruction. The decrease of osteoblasts, due to a disturbance of differentiation, proliferation and apoptosis. Inflammatory mediators are prostaglandin E2 (PGE2, interleukin-1 (IL-1, IL-6 also tumor necrosis alpha (TNF-α stimulates osteoblast apoptosis through gene expression, signaling molecules and receptor-forming osteoblasts. Fish oil of Lemuru, which is widely encountered in Indonesian coast, containing n-3 poly unsaturated fatty acids (n-3 PUFAs are quite high. Consumption of fish oil shown to reduce the expression of PGE2, IL-1, IL-6 and TNF-α. Purpose: The purpose of this study was to examine the effect of Lemuru (Sardinella longicep fish oil on osteoblast apoptosis of rat alveolar bone induced periodontal infection. Methods: Thirty Wistar rats, male, age 5 days, divided into 3 groups: group I rats induced with normal saline, group II rats induced by LPS, and group III rats induced with lemuru fish oil and LPS. Each group was divided into 2 sub-groups that would be sacrified at 13 days and 21 days of age. Fish oil was given at a dose 1ml/300-350 grams. Lipopolysaccharide (LPS induced with the purpose to cause periodontal infection in the maxillary buccal fold molar region with dose 5μl LPS/PBS 0.03 ml. After decapitation and decalcification, the maxilla was cut in 5μm thickness. Apoptosis was analyzed on DNA and detected by TUNEL reaction (transferase-mediated digoxigenin-deoxy-UTP nick end labeling. Results: The results showed that apoptosis of osteoblast cells was significantly smaller in rats induced by Lemuru fish oil. Conclusion: The study showed that Lemuru fish oil reduced the osteoblast apoptosis of rats alveolar bone induced periodontal infection by LPS.Latar belakang: Penyakit periodontal akibat bakteri peridontopatogen, menyebabkan

  6. Leishmania mexicana in Didelphis marsupialis aurita in São Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    E. L. A. Yoshida

    1985-08-01

    Full Text Available Foi identificada pela primeira vez a presença de L. mexicana em Didelphis marsupialis aurita, no Estado de São Paulo — Município de Conchas, através de caracterização bioquímica.

  7. Remotely sensed chlorophyll: A putative trophic link for explaining variability in Indian oil sardine stocks

    Digital Repository Service at National Institute of Oceanography (India)

    George, G.; Meenakumari, B.; Raman, M.; Kumar, S.; Vethamony, P.; Babu, M.T.; Verlecar, X.

    and explain the phenomena of interannual variability. Earlier research has indicated that the probable appearance and disappearance of sardines is an active movement in search of food and favourable conditions. But no specific study has been carried out...

  8. Composition of Bacterial Communities Associated with Aurelia aurita Changes with Compartment, Life Stage, and Population.

    Science.gov (United States)

    Weiland-Bräuer, Nancy; Neulinger, Sven C; Pinnow, Nicole; Künzel, Sven; Baines, John F; Schmitz, Ruth A

    2015-09-01

    The scyphozoan Aurelia aurita is recognized as a key player in marine ecosystems and a driver of ecosystem change. It is thus intensely studied to address ecological questions, although its associations with microorganisms remain so far undescribed. In the present study, the microbiota associated with A. aurita was visualized with fluorescence in situ hybridization (FISH) analysis, and community structure was analyzed with respect to different life stages, compartments, and populations of A. aurita by 16S rRNA gene amplicon sequencing. We demonstrate that the composition of the A. aurita microbiota is generally highly distinct from the composition of communities present in ambient water. Comparison of microbial communities from different developmental stages reveals evidence for life stage-specific community patterns. Significant restructuring of the microbiota during strobilation from benthic polyp to planktonic life stages is present, arguing for a restructuring during the course of metamorphosis. Furthermore, the microbiota present in different compartments of the adult medusa (exumbrella mucus and gastric cavity) display significant differences, indicating body part-specific colonization. A novel Mycoplasma strain was identified in both compartment-specific microbiota and is most likely present inside the epithelium as indicated by FISH analysis of polyps, indicating potential endosymbiosis. Finally, comparison of polyps of different populations kept under the same controlled laboratory conditions in the same ambient water showed population-specific community patterns, most likely due the genetic background of the host. In conclusion, the presented data indicate that the associated microbiota of A. aurita may play important functional roles, e.g., during the life cycle. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  9. Identification of a haptoglobin-hemoglobin complex in the Alaskan Least Cisco (Coregonus sardinella).

    Science.gov (United States)

    Wahl, S M; Boger, J K; Michael, V; Duffy, L K

    1992-01-01

    The hemoglobin and a hemoglobin binding protein have been characterized in the Arctic fish (Coregonus sardinella). The evolutionary significance of the hemoglobin and plasma protein differences between fish and mammals is still unresolved. Blood samples from the Alaskan Least Cisco were separated into plasma and hemoglobin fractions and the proteins in these fractions were analyzed both by alkaline agarose gel electrophoresis, by isolelectric focusing, and by capillary electrophoresis. Staining the plasma proteins gels with o-dianisidine revealed hemoglobin containing protein complexes. A hemoglobin-containing band was observed in hemolyzed plasma which did not migrate with free hemoglobin, and is believed to be hemoglobin-haptoglobin complex. Size exclusion chromatography further characterized the hemoglobin as disassociating freely into dimers, and hemoglobin-haptoglobin complex having a molecular weight greater then 200,000 daltons.

  10. Diagnoses of the State of Exploitation of Small Pelagic Stocks in Senegal by the Use of Size Frequencies: Case of Sardinella%体长频率分析法在塞内加尔沙丁鱼资源开发中的应用

    Institute of Scientific and Technical Information of China (English)

    Fambaye Ngom Sow; Birane Samb; 程和琴

    2010-01-01

    本研究利用Incofish项目开发的可持续发展指标开展渔业资源评估应用研究.研究对象为金色小沙丁鱼(Sardinella aurita)和短体小沙丁鱼(Sardinella maderensis).数据来源于1990年-2005年间塞内加尔个体渔业渔获量及由DR FRIDTJOF NANSEN调查船在塞内加尔海域获得的渔获量.采用Froese(2004年)提出的三个可持续捕捞指标,为渔业资源量的开发状况作出诊断和评估.这些指标包括:渔获物中成鱼的百分比(以100%为最佳)、达到最适开捕体长鱼的百分比(以100%为最佳)和大型产卵鱼的百分比(以0%为最佳,体长大于最适开捕体长1.1倍的雌鱼即为大型产卵鱼).分析结果表明:金色小沙丁鱼处于过度捕捞状态,幼鱼在其渔获物中占有优势地位,捕捞到的金色小沙丁鱼多数小于最适开捕体长,即生长型过度捕捞;短体小沙丁鱼处于充分开发利用状态.总体结果揭示,可持续捕捞指标能够成为替代或完善传统资源评估方法的有用工具.渔业相关的利益主体(包括公众)通过这些指标能够避免过度捕捞,从而更好地理解渔业管理.

  11. Iron fertilisation by Asian dust influences North Pacific sardine regime shifts

    Science.gov (United States)

    Qiu, Yongsong

    2015-05-01

    Forcing factors and mechanisms underlying multidecadal variability in the production of the world's major fish stocks are one of the great mysteries of the oceans. The Japanese and California sardine are species that exhibit the regime shifts. It is shown in the present work that during two periods of frequent Asian dust events over the last 100 years, sardines on opposite sides of the Pacific Ocean only flourished under a dust-active regime. The earlier such regime that peaked in the 1930s was strong, and it brought synchronous changes in the two species that were linked to the frequency of Asian dust events. However, there is an apparent mismatch in the rise and fall of abundance between the two species in the current dust-active regime. The massive increase in Japanese sardine stock in the 1970s was related to high levels of ocean precipitation and strong winter mixing, whereas the stock collapse since 1988 has been attributed to diminished winter mixing. High levels of ocean precipitation in the western North Pacific effectively cause wet deposition of Asian dust and enhance Japanese sardine stock, whereas it reduces dust flux that can be transported to the eastern North Pacific, delaying the increase of California sardine stock. Analysis further indicates that productivity of Japanese sardine stock is jointly controlled by wet deposition of Asian dust and winter mixing, which supplies macronutrients from depth. California sardine productivity is inversely related to precipitation in the western North Pacific and is positively affected by precipitation off western North America. This indicates that Asian dust influx dominates productivity of the species because of iron-limited ocean productivity in the California sardine ranges. The analysis suggests that dust regime shifts influence shifts in sardine productivity regimes and that iron input from Asian dust during trans-Pacific transport is directly responsible. It appears that in addition to enhancing

  12. Abundance, growth and food demand of the scyphomedusa Aurelia aurita in the western Wadden Sea

    Science.gov (United States)

    van der Veer, H. W.; Oorthuysen, W.

    Medusae of Aurelia aurita are found in the western Wadden Sea from the beginning of May till August with maximum numbers of 250 to 500 individuals per 10 3 m 3 during May-June. The existence of a continuous ebb surplus suggests an origin from polyps living in the inner parts of the estuary and a transport or migration of the released medusae towards the North Sea. Growth is fast; a bell size of 20 cm diameter is reached within 3 to 4 months. The species is important as a predator from May to July, reaching maximum carbon biomass values of 12 to 18 g C·10 3 m -3. Predation by A. aurita may affect the recruitment of one of its food sources, viz. fish larvae.

  13. A novel Aurelia aurita protein mesoglein contains DSL and ZP domains.

    Science.gov (United States)

    Matveev, I V; Shaposhnikova, T G; Podgornaya, O I

    2007-09-01

    Body of the scyphoid jellyfish Aurelia aurita consists of 2 epithelia -- epidermis and gastroderm. The layers are separated by a thick layer of extracellular matrix -- mesoglea. A. aurita has a lot of cells in the mesoglea unlike many other Cnidarians. The major protein of the mesoglea with apparent molecular mass of 47 kDa was detected by SDS-PAGE. A partial mRNA of the protein 1421 bp long was cloned and sequenced. The search for homologous nucleotide and protein sequences shows that the mRNA sequence is novel. Deduced amino acid sequence of 416 aa contains zona pellucida (ZP) domain and Delta/Serrate/Lag-2 (DSL) domain. The protein was named mesoglein. According to reverse transcription PCR analysis it is expressed in the mature medusa exclusively in the mesogleal cells. Mesoglein belongs to the lowest phyla among ZP domain-containing proteins. The protein is supposed to be a structural element of the mesoglea extracellular matrix.

  14. Effects of Anadara granosa shell combined with Sardinella longiceps oil on oesteoblast proliferation in bone defect healing process

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    Rima Parwati Sari

    2016-12-01

    Full Text Available Background: Alveolar bone damage is the most common case in dentistry. One way to fix the bone damage is by using bone graft. Anadara granosa shell is a potential bone substitute since it is rich in calcium which can be processed into hydroxyapatite. The addition of Sardinella longiceps oil rich in omega-3 can modulate inflammation, thus accelerating the healing process. Purpose: This study aimed to determine effects of application of Anadara granosa shell combined with Sardinella longiceps oil on osteoblast proliferation in the healing process of bone defects. Method: The subjects were 32 male rats type Wistar divided into 4 groups (n = 8. Making defect was performed on the right bone of the femurs with a half of the diameter of round Mcisinger® Germany bur sized 18. The first group (K is a negative control group that was not given anything. The second group (AG was given Anadara granosa pasta. The third group (AM10 was given Anadara granosa pasta combined with 10% Sardinella longiceps oil. And, the fourth group (AM30 was given Anadara granosa pasta combined with 30% Sardinella longiceps oil. Next, preparations and animal euthanasia were performed on the 7th day after the treatment. The number of osteoblasts then was measured after making preparations for HPA with Hematoxylin eosin staining (HE. Afterward, tabulation of data followed by statistical analysis of Anova and HSD Tukey was carried out. Result: The average number of osteoblasts in Groups K, AG, AM10, and AM30 was 19.00, 34.63, 33.50, and 38.50. The results of Anova test showed a significant difference (p<0.05. Similarly, the results of Tukey-HSD test also showed significant differences (p<0.05 between Group K and all other groups (AG, AM10, and AM30. Nevertheless, there were no significant differences between Group AG and Groups AM10 and AM30, as well as between Group AM10 and Group AM30. Conclusion: The application of the combination of Anadara granosa shell and Sardinella longiceps

  15. Do the Brazilian sardine commercial landings respond to local ocean circulation?

    Science.gov (United States)

    Gouveia, Mainara B; Gherardi, Douglas F M; Lentini, Carlos A D; Dias, Daniela F; Campos, Paula C

    2017-01-01

    It has been reported that sea surface temperature (SST) anomalies, flow intensity and mesoscale ocean processes, all affect sardine production, both in eastern and western boundary current systems. Here we tested the hypothesis whether extreme high and low commercial landings of the Brazilian sardine fisheries in the South Brazil Bight (SBB) are sensitive to different oceanic conditions. An ocean model (ROMS) and an individual based model (Ichthyop) were used to assess the relationship between oceanic conditions during the spawning season and commercial landings of the Brazilian sardine one year later. Model output was compared with remote sensing and analysis data showing good consistency. Simulations indicate that mortality of eggs and larvae by low temperature prior to maximum and minimum landings are significantly higher than mortality caused by offshore advection. However, when periods of maximum and minimum sardine landings are compared with respect to these causes of mortality no significant differences were detected. Results indicate that mortality caused by prevailing oceanic conditions at early life stages alone can not be invoked to explain the observed extreme commercial landings of the Brazilian sardine. Likely influencing factors include starvation and predation interacting with the strategy of spawning "at the right place and at the right time".

  16. Combination of Sardine and Shark Oil High Content of Omega-3 and Squalene

    Directory of Open Access Journals (Sweden)

    Muhamad Musbah

    2017-05-01

    Full Text Available Sardine oil contain high concentration of  EPA but low of  DHA while shark is reverse. Shark oil  high contain of DHA and squalene but low EPA. This research aim to fortify the quality of  fish oil withomega-3 and squalen and improve the quality of fish oil. The combination of fish oil (sardine:shark 1: 1, 1: 2, 1: 3, 1: 4, 2: 1, 3: 1 and 4:1 showed significant results on peroxide, anisidine, and total oxidation value, however free fatty acids analysis did not show the influence to the content value.  The best oxidation parameters value werefound (sardine: shark (1:4 with peroxide was 5.44±0.06 mEq/kg, anisidine was 8.3±0.72 mEq/kg and total oxidation was 19.27±0.7mEq/kg. Fatty acids profile between  sardines and shark oil containedvarious SFA, MUFA and PUFA. Sardine oil which was added more to combination ratio will increase omega-3. Sample 1:4 had 43.16% squalene.

  17. The effects of food and photoperiod on strobilation of Aurelia aurita polyps

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    João Chambel

    2014-06-01

    After 30 days of experiment results showed 100 % survival independently of the treatment. In presence of food the strobilation increased (52.08 ± 4.11 % comparatively with the strobilation in polyps without food (33.33 ± 4.35% (pAurelia aurita. However face the necessity of the optimization of the culture jellyfish conditions for ornamental trade and as source of MNPs, more studies are necessary to turned profitable the mass production of this specie.

  18. Sterile polystyrene culture dishes induce transformation of polyps into medusae in Aurelia aurita (Scyphozoa, Cnidaria).

    Science.gov (United States)

    Herrmann, Klaus; Siefker, Barbara; Berking, Stefan

    2003-01-01

    Polyps of Aurelia aurita produce medusae once a year. Under laboratory conditions this process, termed strobilation, can be induced by lowering the incubation temperature for about two weeks. Here we report a fast induction of strobilation by sterile polystyrene culture dishes. The effect is abolished when the culture dishes are washed twice with hot water prior to the experiment. We recommend that polystyrene cultureware should be pre-washed whenever there is an indication of unusual effects.

  19. Assessment of the sardine (Sardina pilchardus Walbaum, 1792 fishery in the eastern Mediterranean basin (North Aegean Sea

    Directory of Open Access Journals (Sweden)

    K. ANTONAKAKIS

    2012-12-01

    Full Text Available The aim of this study was to describe the biometric characteristics of the European sardine (Sardina pilchardus catches and assess the current status of sardine stock in North Aegean Sea based on population characteristics and abundance trends. The stock was dominated by age groups 1 and 2, not exceeding age group 4. The sardine stock in this area was assessed through an Integrated Catch-at-Age model which implements a separable Virtual Population Analysis on catch at age data with weighted tuning indices. Sardine landings data derived from the commercial purse seine fishery over the period 2000-2008 were combined with the age structure of the stock as resulted from fisheries independent acoustic surveys. Sensitivity analysis of the impact of natural mortality values on stock assessment results was applied. Additionally forecast of the sardine population parameters and catches under different exploitation scenarios was implemented in a medium term basis. Results indicated that the North Aegean Sea sardine stock is considered fully exploited with the fishery operating close but over the empirical exploitation level for sustainability. Finally, the status of the sardine stock in N. Aegean Sea is discussed in relation to the sardine stocks from the western and the central Mediterranean basin.

  20. Assessment of the sardine (Sardina pilchardus Walbaum, 1792 fishery in the eastern Mediterranean basin (North Aegean Sea

    Directory of Open Access Journals (Sweden)

    K. ANTONAKAKIS

    2011-06-01

    Full Text Available The aim of this study was to describe the biometric characteristics of the European sardine (Sardina pilchardus catches and assess the current status of sardine stock in North Aegean Sea based on population characteristics and abundance trends. The stock was dominated by age groups 1 and 2, not exceeding age group 4. The sardine stock in this area was assessed through an Integrated Catch-at-Age model which implements a separable Virtual Population Analysis on catch at age data with weighted tuning indices. Sardine landings data derived from the commercial purse seine fishery over the period 2000-2008 were combined with the age structure of the stock as resulted from fisheries independent acoustic surveys. Sensitivity analysis of the impact of natural mortality values on stock assessment results was applied. Additionally forecast of the sardine population parameters and catches under different exploitation scenarios was implemented in a medium term basis. Results indicated that the North Aegean Sea sardine stock is considered fully exploited with the fishery operating close but over the empirical exploitation level for sustainability. Finally, the status of the sardine stock in N. Aegean Sea is discussed in relation to the sardine stocks from the western and the central Mediterranean basin.

  1. Odontella aurita-enriched diet prevents high fat diet-induced liver insulin resistance.

    Science.gov (United States)

    Amine, Hamza; Benomar, Yacir; Haimeur, Adil; Messaouri, Hafida; Meskini, Nadia; Taouis, Mohammed

    2016-01-01

    The beneficial effect of polyunsaturated omega-3 fatty acid (w-3 FA) consumption regarding cardiovascular diseases, insulin resistance and inflammation has been widely reported. Fish oil is considered as the main source of commercialized w-3 FAs, and other alternative sources have been reported such as linseed or microalgae. However, despite numerous reports, the underlying mechanisms of action of w-3 FAs on insulin resistance are still not clearly established, especially those from microalgae. Here, we report that Odontella aurita, a microalga rich in w-3 FAs eicosapentaenoic acid, prevents high fat diet-induced insulin resistance and inflammation in the liver of Wistar rats. Indeed, a high fat diet (HFD) increased plasma insulin levels associated with the impairment of insulin receptor signaling and the up-regulation of toll-like receptor 4 (TLR4) expressions. Importantly, Odontella aurita-enriched HFD (HFOA) reduces body weight and plasma insulin levels and maintains normal insulin receptor expression and responsiveness. Furthermore, HFOA decreased TLR4 expression, JNK/p38 phosphorylation and pro-inflammatory factors. In conclusion, we demonstrate for the first time, to our knowledge, that diet supplementation with whole Ondontella aurita overcomes HFD-induced insulin resistance through the inhibition of TLR4/JNK/p38 MAP kinase signaling pathways.

  2. Respiratory response to temperature of three populations of Aurelia aurita polyps in northern Europe.

    Science.gov (United States)

    Höhn, Danja P; Lucas, Cathy H; Thatje, Sven

    2017-01-01

    The benthic life stage (polyp or scyphistoma) of the bloom-forming jellyfish, Aurelia aurita (Linnaeus, 1759), also known as the moon jellyfish, contributes to the seasonal occurrence and abundance of medusa blooms via asexual reproduction. A. aurita is widely distributed in coastal areas in northern Europe, and one of the most studied jellyfish species. While the physiology of the visible medusa is largely understood, understanding of the physiology of the perennial benthic life-stage is scarce. To measure the physiological tolerance of A. aurita, the scyphistoma's temperature sensitivity across its distributional range was investigated. Respiration rates of polyps from three northern European locations exposed to 11 temperatures between 2 and 22°C were measured. There was a significant difference in respiration rate among the three polyp populations, which may reflect on differences in their thermal tolerance window. A critical temperature was reached at 14°C with the metabolic rate decreasing below and above that temperature. This pattern was less pronounced in the Norwegian population but polyps were able to survive, at least temporarily, those temperatures exceeding their natural range. While polyps collected from northern Norway, with a narrow environmental thermal window, displayed a low baseline metabolism with a Q10 value of 1.2, polyps from southern England and Scotland had Q10 values of 1.6 and 2.5, respectively. Differences in polyps' respiration rates across their distributional range suggest that populations have evolved adaptations to local environmental thermal conditions.

  3. Production, Characterization, and Antioxidant Activity of Fucoxanthin from the Marine Diatom Odontella aurita

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    Chengwu Zhang

    2013-07-01

    Full Text Available The production, characterization, and antioxidant capacity of the carotenoid fucoxanthin from the marine diatom Odontella aurita were investigated. The results showed that low light and nitrogen-replete culture medium enhanced the biosynthesis of fucoxanthin. The maximum biomass concentration of 6.36 g L−1 and maximum fucoxanthin concentration of 18.47 mg g−1 were obtained in cultures grown in a bubble column photobioreactor (Ø 3.0 cm inner diameter, resulting in a fucoxanthin volumetric productivity of 7.96 mg L−1 day−1. A slight reduction in biomass production was observed in the scaling up of O. aurita culture in a flat plate photobioreactor, yet yielded a comparable fucoxanthin volumetric productivity. A rapid method was developed for extraction and purification of fucoxanthin. The purified fucoxanthin was identified as all-trans-fucoxanthin, which exhibited strong antioxidant properties, with the effective concentration for 50% scavenging (EC50 of 1,1-dihpenyl-2-picrylhydrazyl (DPPH radical and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS radical being 0.14 and 0.03 mg mL−1, respectively. Our results suggested that O. aurita can be a natural source of fucoxanthin for human health and nutrition.

  4. A biophysical model of S. aurita early life history in the northern Gulf of Guinea

    Science.gov (United States)

    Koné, Vamara; Lett, Christophe; Penven, Pierrick; Bourlès, Bernard; Djakouré, Sandrine

    2017-02-01

    S. aurita is the most abundant small pelagic fish in the northern Gulf of Guinea. Its reproduction and recruitment depend crucially on environmental conditions. We developed a biophysical model of S. aurita early life history by coupling offline an individual-based model with the regional oceanic modeling system (ROMS). We used this model to investigate the main factors driving variability in eggs and larval dispersal and survival in the northern Gulf of Guinea. Precisely, individuals were released from different spawning areas along the coast and tracked for a period of 28 days corresponding to their planktonic phase. Individuals that remained in the coastal recruitment areas at an age more than 7 days, at which they can supposedly actively retain themselves in a favorable area, were considered as recruited. Simulation results show the importance of the spawning areas around Cape Palmas and Cape Three Points where cyclonic eddies trap eggs and larvae along the coast, preventing their advection offshore by the Guinea Current. The spawning period also plays a key role in the recruitment success, with highest coastal retention obtained during the major upwelling period (July-September). We find that a second retention peak can occur during the minor upwelling period (February-March) when larval mortality due to temperature is included in the model. These results are in general agreement with knowledge of S. aurita reproduction in the northern Gulf of Guinea.

  5. Effects of Climate Change on Sardine Productivity in the California Current System

    Science.gov (United States)

    Baumgartner, T. R.; Auad, G.; Miller, A. J.

    2007-05-01

    The Pacific sardine (Sardinops sagax caeruleus) is one of several coastal pelagic, planktivorous species of fish that provide important trophic links within the ecosystems of the major eastern and western boundary currents. Significant and persistent change in sardine productivity has occurred in the California Current over interdecadal periods in response to reorganization of basin-wide, ocean-atmosphere circulation. Less extreme, but still significant changes in sardine productivity are associated with interannual to decadal-scale climate variability. A precipitous decline of the sardine population began in the mid-1940s with a shift in climate leading to cooling of the California Current system. While the decline, and ultimately the collapse of the population, was exacerbated by intensive fishing, the sardine also suffered a severe reduction in productivity with the southward contraction of favorable thermal habitat that led to restriction of the population to the waters off Southern California and Baja California. This southward displacement resulted in geographic separation of the population from the region off central and northern California that is characterized by significantly higher concentrations of zooplankton that supported the previous levels of success in spawning and larval development. The climate shift in 1976-77 led to the recovery of the population and extension of its range of distribution northwards into the waters off British Columbia. The relation of reproductive success of the sardine population to interannual and decadal climate change was examined for the period 1982-2005 using a suite of seasonal indices representing climate processes and habitat conditions (including zooplankton food levels) occurring through the different stages in the sardine life cycle. We used both stepwise regression and EOF analyses to determine the association between levels of recruitment success and seasonal indices of the Pacific Decadal Oscillation, Ekman

  6. EFFECT OF PRESSING ON THE SHELF LIFE OF SUNDRIED WHITE SARDINE (ESCUALOSA THORACATA

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    M.B. Priyadarshini

    2012-07-01

    Full Text Available In the present study, an attempt was made to investigate and explore a method for preparation of salted and dried white sardine which would have organoleptically sound attributes viz., color, flavor, taste and texture. It could however be concluded from the results of present study that the sun dried pressed samples were in better condition than the unpressed sample.

  7. Temporal genetic variation as revealed by a microsatellite analysis of European sardine ( Sardina pilchardus) archived samples

    DEFF Research Database (Denmark)

    Ruggeri, Paolo; Splendiani, Andrea; Bonanomi, Sara;

    2012-01-01

    The Adriatic stock of European sardine ( Sardina pilchardus) has experienced large interannual demographic fluctuations over the last 30 years, with a severe decline beginning in 1991 and continuing until 1997. In the present study, six microsatellite loci were used on a time series collection of...

  8. [Fatty acids in sardine canned in tomato sauce from different fishing areas of the Mexican Pacific].

    Science.gov (United States)

    Castro Gónzalez, M I; Montaño Benavides, S; Pérez-Gil Romo, F

    2001-12-01

    Numerous investigations have pointed out the importance that the fatty acids have in the process health-illness, and that the marine resources are excellent sources of the series omega 3 and omega 6. In Mexico, the sardine is a product of marine origin of wide consumption due to its high readiness and low cost. The objective of the present study was to determine the fatty acids profile (FA) in sardine canned in tomato sauce coming from different fishing areas (A) of the Mexican Pacific. There were randomly obtained 8 commercial mark (5 cans of each mark) of sardine canned in tomato sauce; they were classified in sardine of South Baja California Sur (A1), Sonora (A2) and Sinaloa (A3). The samples without draining were liquified and thereafter were obtained the methyl esters of fatty acids that were analyzed by gas chromatography with a flame ionization detector. In all the areas they were identified and quantified as 3 FA omega 3 (linolenic, EPA and DHA) and 2 AG omega 6 (linoleic and arachidonic); this source is rich in FA monounsaturated and also presents a considerable quantity of trans FA (18:1n9t and 18:2n6t). The DHA was the most abundant AG in all the areas (3064-4704 mg/100 g); finally, the relationships omega 3/omega 6 were from 3.5 (A1) up to 8.9 (A3). In conclusion, sardine canned in tomato sauce of the mexican Pacific is a rich food in omega-3 and omega-6 FA, independently of the processing area.

  9. Keeping quality of vacuum-packed smoked sardine fillets: microbiological aspects.

    Science.gov (United States)

    Beltrán, A; Peláez, C; Moral, A

    1989-03-01

    The effect of mixed smoking (2 h at 30 degrees C and 45 min at 75 degrees C was studied on the keeping quality of lean and fatty sardine fillets from fish caught in the Mediterranean Sea in March and June, respectively. Raw material for smoking included both fresh and frozen sardine fillets. Microbiological and sensory analyses were performed over the storage period, which lasted for 120 days at 0 +/- 1 degrees C. Smoking reduced the viable microbial flora values from 3-5 x 10(4) cfu/g to 2 x 10(2) cfu/g. The antimicrobial action of smoking, combined with that of vacuum packaging, had the effect of producing a gram-positive flora made up mainly of lactic acid bacteria. A practical consequence of this was to extend the shelf life of the smoked sardines for a period of 4 months. Frozen storage of samples at -18 degrees C for 6 months prior to smoking affected the behaviour of the microbial flora during cold storage at 0 degrees C, but did not affect the behaviour of the flora over a period of 3 months frozen storage, since the initial numbers of bacteria decreased and microbial growth was retarded. Furthermore, at the end of the storage period, the number of bacteria in the samples of frozen fatty sardines were higher than in the samples of frozen lean sardine. Physical conditions, therefore appear to exert an influence on bacterial growth. Sulphite-reducing Clostridia were not detected during the storage period.

  10. Rock preference of planulae of jellyfish Aurelia aurita (Linnaeus 1758) for settlement in the laboratory

    Science.gov (United States)

    Yoon, Won Duk; Choi, Sung-Hwan; Han, Changhoon; Park, Won Gyu

    2014-06-01

    Planulae of Aurelia aurita were exposed to 11 types of rocks (basalt, gabbro, granite, rhyolite, sandstone, limestone, conglomerate, gneiss, quartzite, marble and schist) to examine their attachment preference among rock material and position. Numbers of attached polyps was the highest on marble and the least on limestone. Their preference with regard to settling position was the same among the rocks, showing the highest density of polyps on the underside (88.5%) compared to upper (23.6%) and perpendicular sides (10.3%) of rock. The results showed that while position preference is more important than rock property, higher numbers of polyps were observed in rocks with a medium surface hardness.

  11. Bacterially induced stolon settlement in the scyphopolyp of Aurelia aurita (Cnidaria, Scyphozoa)

    Science.gov (United States)

    Schmahl, G.

    1985-03-01

    Unsettled stoloniferous scyphopolyps of Aurelia aurita Lamarck were offered different substrates for settlement under defined conditions. On addition of different biogenic and abiotic substrates, a pure strain of bacteria, a species of Micrococcaceae, was observed to trigger the settlement of the stolon. The settlement reaction only takes place following direct contact with the bacteria; sterile filtrated culture medium of the same bacterial strain was not able to induce settlement. The bacteria were found to be effective on stolon settlement during the logarithmic growth phase, but not during the stationary phase.

  12. Seasonal comparison of the diets of juvenile European anchovy Engraulis encrasicolus and sardine Sardina pilchardus in the Gulf of Lions

    Science.gov (United States)

    Costalago, David; Palomera, Isabel; Tirelli, Valentina

    2014-05-01

    Anchovy and sardine in the Mediterranean are known to share the same habitat and, consequently, to interact with one another. These two sympatric pelagic species are planktivorous and consume a wide range of planktonic prey items during all their developmental stages, potentially overlapping their ecological niches, although the feeding interactions between these species have been poorly investigated. Here we compare the dietary habits of the juvenile phases of anchovy and sardine during different seasons in the northwestern Mediterranean Sea, through analysis of their stomach contents and of their feeding-related anatomical characteristics. In this study we show that juveniles of anchovy and sardine do not compete for food, and we describe significant dietary differences between anchovy and sardine due to their different alimentary tract morphology.

  13. Indomethacin induction of metamorphosis from the asexual stage to sexual stage in the moon jellyfish, Aurelia aurita.

    Science.gov (United States)

    Kuniyoshi, Hisato; Okumura, Izumi; Kuroda, Rie; Tsujita, Natsumi; Arakawa, Kenji; Shoji, Jun; Saito, Tamio; Osada, Hiroyuki

    2012-01-01

    We found while screening a chemical library that indomethacin, an inhibitor of prostaglandin biosynthesis, induced strobilation (metamorphosis from the asexual to sexual stage) in the moon jellyfish, Aurelia aurita. Indomethacin initiated strobilation in a dose-dependent manner, but was not involved in the progression of strobilation. Pharmacological experiments suggested that indomethacin could induce strobilation independently of prostaglandin biosynthesis.

  14. Gill damage to Atlantic salmon (Salmo salar caused by the common jellyfish (Aurelia aurita under experimental challenge.

    Directory of Open Access Journals (Sweden)

    Emily J Baxter

    Full Text Available BACKGROUND: Over recent decades jellyfish have caused fish kill events and recurrent gill problems in marine-farmed salmonids. Common jellyfish (Aurelia spp. are among the most cosmopolitan jellyfish species in the oceans, with populations increasing in many coastal areas. The negative interaction between jellyfish and fish in aquaculture remains a poorly studied area of science. Thus, a recent fish mortality event in Ireland, involving Aurelia aurita, spurred an investigation into the effects of this jellyfish on marine-farmed salmon. METHODOLOGY/PRINCIPAL FINDINGS: To address the in vivo impact of the common jellyfish (A. aurita on salmonids, we exposed Atlantic salmon (Salmo salar smolts to macerated A. aurita for 10 hrs under experimental challenge. Gill tissues of control and experimental treatment groups were scored with a system that rated the damage between 0 and 21 using a range of primary and secondary parameters. Our results revealed that A. aurita rapidly and extensively damaged the gills of S. salar, with the pathogenesis of the disorder progressing even after the jellyfish were removed. After only 2 hrs of exposure, significant multi-focal damage to gill tissues was apparent. The nature and extent of the damage increased up to 48 hrs from the start of the challenge. Although the gills remained extensively damaged at 3 wks from the start of the challenge trial, shortening of the gill lamellae and organisation of the cells indicated an attempt to repair the damage suffered. CONCLUSIONS: Our findings clearly demonstrate that A. aurita can cause severe gill problems in marine-farmed fish. With aquaculture predicted to expand worldwide and evidence suggesting that jellyfish populations are increasing in some areas, this threat to aquaculture is of rising concern as significant losses due to jellyfish could be expected to increase in the future.

  15. Gill damage to Atlantic salmon (Salmo salar) caused by the common jellyfish (Aurelia aurita) under experimental challenge.

    Science.gov (United States)

    Baxter, Emily J; Sturt, Michael M; Ruane, Neil M; Doyle, Thomas K; McAllen, Rob; Harman, Luke; Rodger, Hamish D

    2011-04-07

    Over recent decades jellyfish have caused fish kill events and recurrent gill problems in marine-farmed salmonids. Common jellyfish (Aurelia spp.) are among the most cosmopolitan jellyfish species in the oceans, with populations increasing in many coastal areas. The negative interaction between jellyfish and fish in aquaculture remains a poorly studied area of science. Thus, a recent fish mortality event in Ireland, involving Aurelia aurita, spurred an investigation into the effects of this jellyfish on marine-farmed salmon. To address the in vivo impact of the common jellyfish (A. aurita) on salmonids, we exposed Atlantic salmon (Salmo salar) smolts to macerated A. aurita for 10 hrs under experimental challenge. Gill tissues of control and experimental treatment groups were scored with a system that rated the damage between 0 and 21 using a range of primary and secondary parameters. Our results revealed that A. aurita rapidly and extensively damaged the gills of S. salar, with the pathogenesis of the disorder progressing even after the jellyfish were removed. After only 2 hrs of exposure, significant multi-focal damage to gill tissues was apparent. The nature and extent of the damage increased up to 48 hrs from the start of the challenge. Although the gills remained extensively damaged at 3 wks from the start of the challenge trial, shortening of the gill lamellae and organisation of the cells indicated an attempt to repair the damage suffered. Our findings clearly demonstrate that A. aurita can cause severe gill problems in marine-farmed fish. With aquaculture predicted to expand worldwide and evidence suggesting that jellyfish populations are increasing in some areas, this threat to aquaculture is of rising concern as significant losses due to jellyfish could be expected to increase in the future.

  16. Effects of nutrients and light intensity on the growth and biochemical composition of a marine microalga Odontella aurita

    Science.gov (United States)

    Xia, Song; Wan, Linglin; Li, Aifen; Sang, Min; Zhang, Chengwu

    2013-11-01

    Algal biotechnology has advanced greatly in the past three decades. Many microalgae are now cultivated to produce bioactive substances. Odontella aurita is a marine diatom industrially cultured in outdoor open ponds and used for human nutrition. For the first time, we have systematically investigated the effects of culture conditions in cylindrical glass columns and flat-plate photobioreactors, including nutrients (nitrogen, phosphorus, silicon, and sulfur), light intensity and light path, on O. aurita cell growth and biochemical composition (protein, carbohydrate, β-1,3-glucan, lipids, and ash). The optimal medium for photoautotrophic cultivation of O. aurita contained 17.65 mmol/L nitrogen, 1.09 mmol/L phosphorus, 0.42 mmol/L silicon, and 24.51 mmol/L sulfur, yielding a maximum biomass production of 6.1-6.8 g/L and 6.7-7.8 g/L under low and high light, respectively. Scale-up experiments were conducted with flat-plate photobioreactors using different light-paths, indicating that a short light path was more suitable for biomass production of O. aurita. Analyses of biochemical composition showed that protein content decreased while carbohydrate (mainly composed of β-1,3-glucan) increased remarkably to about 50% of dry weight during the entire culture period. The highest lipid content (19.7% of dry weight) was obtained under 0.11 mmol/L silicon and high light conditions at harvest time. Fatty acid Profiles revealed that 80% were C14, C16, and C20, while arachidonic acid and eicosapentaenoic acid (EPA) accounted for 1.6%-5.6% and 9%-20% of total fatty acids, respectively. High biomass production and characteristic biochemical composition Profiles make O. aurita a promising microalga for the production of bioactive components, such as EPA and β-1,3-glucan.

  17. HUBUNGAN PANJANG BOBOT, FAKTOR KONDISI DAN STRUKTUR UKURAN IKAN LEMURU (Sardinella lemuru DI PERAIRAN SELAT BALI

    Directory of Open Access Journals (Sweden)

    Arief Wujdi

    2016-03-01

    Full Text Available Ikan lemuru merupakan jenis ikan hasil tangkapan utama kegiatan perikanan di perairan Selat Bali yang status pemanfaatannya sudah mengalami lebih tangkap dan memerlukan upaya pengelolaan. Penelitian ini bertujuan untuk mengetahui hubungan panjang dan bobot, faktor kondisi, serta struktur ukuran ikan lemuru di perairan Selat Bali. Pengumpulan data dilakukan pada bulan Agustus 2010 hingga Desember 2011 dengan metode survei dan pengamatan langsung di lapangan. Hasil penelitian menunjukkan bahwa hubungan panjang dan bobot ikan lemuru mengikuti persamaan W=0,007FL3,167 dan memiliki pola pertumbuhan allometrik positif (b>3 namun pada setiap bulannya mengalami perubahan pola pertumbuhan. Nilai faktor kondisi relatif berkisar antara 0,95-1,28 dan berfluktuasi setiap bulannya. Hasil ini diduga dipengaruhi oleh ketersediaan makanan. Ikan lemuru berukuran kecil atau “sempenit” (<11 cmFL banyak tertangkap pada bulan Agustus dan September 2010 serta Juli dan November 2011 dan diduga pada waktu tersebut terjadi awal rekruitmen.   Bali sardinella (“lemuru” is mostly caught by fishers in the Bali Strait waters, and its status predicted have overfishing so that it needs to manage this resources. The purpose of this study to investigate the length-weight relationship, the condition factors and size structure of lemuru in the Bali Strait waters. The data was collected through survey and direct observation in the field from August 2010 to December 2011. The result of this study shows that length-weight relationship could be described as W = 0.007 FL3, 167. Nevertheless,  it is change on the growth pattern by monthly. The value of relative condition factors of lemuru were ranging from 0.95 to 1.28 and very fluctuated by monthly. It is predicted that influenced by feed availability in Bali Strait waters. The smaal size of lemuru, namely “sempenit (<11 cmFL are dominantly caught during August to September 2010 and July and November 2011 that indicated

  18. Monitoring the Freshness of Moroccan Sardines with a Neural-Network Based Electronic Nose

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    Benachir Bouchikhi

    2006-10-01

    Full Text Available An electronic nose was developed and used as a rapid technique to classify thefreshness of sardine samples according to the number of days spent under cold storage (4 ±1°C, in air. The volatile compounds present in the headspace of weighted sardine sampleswere introduced into a sensor chamber and the response signals of the sensors wererecorded as a function of time. Commercially available gas sensors based on metal oxidesemiconductors were used and both static and dynamic features from the sensorconductance response were input to the pattern recognition engine. Data analysis wasperformed by three different pattern recognition methods such as probabilistic neuralnetworks (PNN, fuzzy ARTMAP neural networks (FANN and support vector machines(SVM. The objective of this study was to find, among these three pattern recognitionmethods, the most suitable one for accurately identifying the days of cold storage undergoneby sardine samples. The results show that the electronic nose can monitor the freshness ofsardine samples stored at 4°C, and that the best classification and prediction are obtainedwith SVM neural network. The SVM approach shows improved classificationperformances, reducing the amount of misclassified samples down to 3.75 %.

  19. Numerical study of the seasonal variability of Aurelia aurita ephyra abundance: Application to the JiaoZhou Bay, China

    Science.gov (United States)

    Hu, Z.; Wang, N.; Li, C.; Sun, S.

    2013-12-01

    The outbreaks of the inshore scyphozoan Aurelia aurita have been increasingly reported in the coastal waters of China in recent years, with detrimental impacts on local ecosystems and economies. The strobilation process is an important part of the A. aurita life cycle because it can increase the jellyfish population size by producing new jellyfish (ephyrae). Hence it is essential to investigate the strobilation dynamics in order to better understand and possibly predict jellyfish outbreaks. In this work, we present a model that provides a realistic simulation of the combined effects of temperature and food supply on the ephyra liberation dynamics. The parameterization of the model is based on the laboratory and field experiments and the literature. The model results show that temperature has a parabolic-like effect on liberated ephyra numbers. The numbers of liberated ephyra increase with temperature before reaching a peak and then decays. No ephyrae liberation occurs in very low or very high temperautres, despite the food level is high or not. While liberation occurs, high food supply increases ephyra production. Application of the model to JiaoZhou Bay with in-situ measured temperature and zooplanton abundance produces results coherent with the seasonal in situ observations. In JiaoZhou Bay, the ephyrae of A. aurita usually appear in early April, and reaches their peak in abundance in June and the jellyfish outbreaks reported occurs in July-August. Experiments with the model show that there are two main periods of strobilation in JiaoZhou Bay. The first begins at early march and reaches its peak in May. The second occurs during October to early December with its peak in November. The first strobilation period is in a good agreement with in situ observations. No available data in JiaoZhou Bay could be used to validate the second one. Nevertheless, occurrence of A. aurita ephyrae was reported in late October in similar latitudes such as in Tokyo Bay. No jellyfish

  20. Life-history stages of the jellyfish Aurelia aurita - towards a demographic understanding of jellyfish blooms

    DEFF Research Database (Denmark)

    Goldstein, Josephine

    2017-01-01

    Jellyfish blooms are conspicuous demographic events that have received increasing attention by the public and the scientific community over the last decades due to their negative impact on fisheries, tourism and other human industries. Several aspects of the complex life cycles of the jellyfish...... species involved in mass occurrence have been covered in previous studies, providing important insight into diverse life stage-specific adaptation strategies to changing environmental conditions. Quantification of the demographic rates that characterize different life-history stages however has remained...... population dynamics and may inspire future research to sharpen an integrated view of jellyfish life histories in an ever changing environment. Such insight might be of particular importance beyond A. aurita in times of habitat eutrophication, climate warming and changes in species diversity....

  1. Molecular characterization of aspartylglucosaminidase, a lysosomal hydrolase upregulated during strobilation in the moon jellyfish, Aurelia aurita.

    Science.gov (United States)

    Tsujita, Natsumi; Kuwahara, Hiroyuki; Koyama, Hiroki; Yanaka, Noriyuki; Arakawa, Kenji; Kuniyoshi, Hisato

    2017-05-01

    The life cycle of the moon jellyfish, Aurelia aurita, alternates between a benthic asexual polyp stage and a planktonic sexual medusa (jellyfish) stage. Transition from polyp to medusa is called strobilation. To investigate the molecular mechanisms of strobilation, we screened for genes that are upregulated during strobilation using the differential display method and we identified aspartylglucosaminidase (AGA), which encodes a lysosomal hydrolase. Similar to AGAs from other species, Aurelia AGA possessed an N-terminal signal peptide and potential N-glycosylation sites. The genomic region of Aurelia AGA was approximately 9.8 kb in length and contained 12 exons and 11 introns. Quantitative RT-PCR analysis revealed that AGA expression increased during strobilation, and was then decreased in medusae. To inhibit AGA function, we administered the lysosomal acidification inhibitors, chloroquine or bafilomycin A1, to animals during strobilation. Both inhibitors disturbed medusa morphogenesis at the oral end, suggesting involvement of lysosomal hydrolases in strobilation.

  2. Structural analysis of O-glycans of mucin from jellyfish (Aurelia aurita) containing 2-aminoethylphosphonate.

    Science.gov (United States)

    Urai, Makoto; Nakamura, Takemichi; Uzawa, Jun; Baba, Takayuki; Taniguchi, Kayoko; Seki, Hiroko; Ushida, Kiminori

    2009-11-02

    The structure of O-glycan in qniumucin (Q-mucin), which is a novel mucin extracted from jellyfish, was analyzed by a combination of NMR and ESI-MS/MS. A previously unidentified monosaccharide involved in the glycan chains was determined to be N-acetylgalactosamine (GalNAc) substituted by 2-aminoethylphosphonate (AEP) at the C-6. The O-glycans in Q-mucin from Aurelia aurita were proved to be mainly composed of three monosaccharides: GalNAc, AEP-(O-->6)-GalNAc, and P-6-GalNAc. To the best of our knowledge, this is the first example of an O-glycan structure of glycoproteins containing AEP. This exceptionally simple structure of Q-mucin and its potential use in material science and technology are revealed.

  3. Flexible margin kinematics and vortex formation of Aurelia aurita and Robojelly.

    Science.gov (United States)

    Villanueva, Alex; Vlachos, Pavlos; Priya, Shashank

    2014-01-01

    The development of a rowing jellyfish biomimetic robot termed as "Robojelly", has led to the discovery of a passive flexible flap located between the flexion point and bell margin on the Aurelia aurita. A comparative analysis of biomimetic robots showed that the presence of a passive flexible flap results in a significant increase in the swimming performance. In this work we further investigate this concept by developing varying flap geometries and comparing their kinematics with A. aurita. It was shown that the animal flap kinematics can be replicated with high fidelity using a passive structure and a flap with curved and tapered geometry gave the most biomimetic performance. A method for identifying the flap location was established by utilizing the bell curvature and the variation of curvature as a function of time. Flaps of constant cross-section and varying lengths were incorporated on the Robojelly to conduct a systematic study of the starting vortex circulation. Circulation was quantified using velocity field measurements obtained from planar Time Resolved Digital Particle Image Velocimetry (TRDPIV). The starting vortex circulation was scaled using a varying orifice model and a pitching panel model. The varying orifice model which has been traditionally considered as the better representation of jellyfish propulsion did not appear to capture the scaling of the starting vortex. In contrast, the pitching panel representation appeared to better scale the governing flow physics and revealed a strong dependence on the flap kinematics and geometry. The results suggest that an alternative description should be considered for rowing jellyfish propulsion, using a pitching panel method instead of the traditional varying orifice model. Finally, the results show the importance of incorporating the entire bell geometry as a function of time in modeling rowing jellyfish propulsion.

  4. Population Dynamics of Jellyfish Aurelia aurita (s.l. in Sihwa Lake

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    Hyun-pyo Hong

    2013-09-01

    Full Text Available We investigated the population dynamics of Aurelia aurita in Sihwa Lake from April to October in 2009. Salinity ranged from 5.9 to 30.7 psu at the surface. Abundance of mesozooplankton ranged from 3 to 111,874 indiv. m−3. Ephyrae occurred from April to May with the peak in abundance occurring on 17 April. Maximum density of ephyrae was observed near the power transmission towers that are known to be habitats of polyps. Mortality of ephyrae was lower than in other areas because of the abundant prey concentration and the absence of predators. Young medusae occurred from April to July with the peak in abundance occurring on 8 May. Adult medusae occurred from May to July with the peak in abundance on 25 June and they disappeared before the rainy season. Planula occurred only in May and June with the peak in abundance on 25 June. Growth rates of Aurelia aurita ranged from −0.06 to 0.34 d−1, and decreased rapidly after May. The period in which adult medusa occurred was restricted, compared with those in other area in Korea (e.g., Masan Bay and Japan (e.g., Tokyo Bay. In the period of this study, the available food was limited in June and salinity decreased to ca. 20 psu in May because of the beginning of the wet season. We assumed that the exceptionally short period of occurrence of the medusa may be a response of adults to changes in temperature, salinity or food limitation which leads to the precocious maturation of young medusa and the release of planula and that the brief occurrence of medusa was caused by an abrupt decrease in activity after the release of planula.

  5. Flexible margin kinematics and vortex formation of Aurelia aurita and Robojelly.

    Directory of Open Access Journals (Sweden)

    Alex Villanueva

    Full Text Available The development of a rowing jellyfish biomimetic robot termed as "Robojelly", has led to the discovery of a passive flexible flap located between the flexion point and bell margin on the Aurelia aurita. A comparative analysis of biomimetic robots showed that the presence of a passive flexible flap results in a significant increase in the swimming performance. In this work we further investigate this concept by developing varying flap geometries and comparing their kinematics with A. aurita. It was shown that the animal flap kinematics can be replicated with high fidelity using a passive structure and a flap with curved and tapered geometry gave the most biomimetic performance. A method for identifying the flap location was established by utilizing the bell curvature and the variation of curvature as a function of time. Flaps of constant cross-section and varying lengths were incorporated on the Robojelly to conduct a systematic study of the starting vortex circulation. Circulation was quantified using velocity field measurements obtained from planar Time Resolved Digital Particle Image Velocimetry (TRDPIV. The starting vortex circulation was scaled using a varying orifice model and a pitching panel model. The varying orifice model which has been traditionally considered as the better representation of jellyfish propulsion did not appear to capture the scaling of the starting vortex. In contrast, the pitching panel representation appeared to better scale the governing flow physics and revealed a strong dependence on the flap kinematics and geometry. The results suggest that an alternative description should be considered for rowing jellyfish propulsion, using a pitching panel method instead of the traditional varying orifice model. Finally, the results show the importance of incorporating the entire bell geometry as a function of time in modeling rowing jellyfish propulsion.

  6. PENGELOLAAN DAN PROFITABILITAS USAHA PENANGKAPAN LEMURU (SARDINELLA LEMURU BLEEKER,1853 DI SELAT BALI

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    Hesti Warih Madyeng Ratri

    2012-11-01

    Full Text Available Penangkapan lemuru (Sardinella lemuru Bleeker,1853 di Selat Bali telah dilakukan jauh sebelum diperkenalkannya purse seine dua kapal (slerek yang mempunyai hasil tangkapan menggembirakan. Hasil studi sejak tahun 1974 menyimpulkan bahwa stok lemuru di Selat Bali dieksploitasi sangat intensif, sehingga mengancam kelangsungan stok lemuru dan melemahkan keuntungan sumberdaya. Pengelolaan perikanan lemuru di Selat Bali yang telah dilakukan sejak tahun 1977 dan terus diperbaharui sampai tahun 1992 perlu ditinjau kembali, mengingat peraturan dimaksud sudah tidak sesuai dengan kondisi di lapangan dan tidak dipatuhi oleh para pemangku kepentingan. Nelayan mematuhi aturan tentang pembatasan jumlah kapal, namun ukuran kapal, alat tangkap, jumlah ABK dan alat bantu lampu dilakukan penambahan besar-besaran. Peningkatan upaya ini dikhawatirkan akan mengancam keberlanjutan usaha. Oleh karena itu, diperlukan kajian untuk menganalisis profitabilitas usaha penangkapan dan masukan konkrit guna pengelolaan perikanan lemuru di Selat Bali. Sintesis hasil penelitian terdahulu di Selat Bali dan diperluas dengan data hasil observasi lapang dengan menggunakan model Max Aquero. Keuntungan total ekonomi usaha penangkapan di Selat Bali pada tahun 2007 mencapai Rp 205.856.283.770,- dengan hasil tangkapan lemuru sebanyak 254.691.315 kg dan tenaga kerja yang terlibat sebanyak 30.583 orang. Hasil tangkapan lemuru yang di publikasi oleh Provinsi Bali dan Jawa Timur adalah data yang tidak akurat. Mengingat informasi dari data tersebut merupakan landasan kebijakan, langkah konkrit perbaikan pengelolaan perikanan lemuru Selat Bali yang mendesak adalah perbaikan metode pendataan dan sumberdaya manusianya. The catching of lemuru in Bali Strait had been done long before two vessels purse seine (slerek which gave satisfactory result was introduced. The outcome of numerous studies conducted since 1976 concluded that lemuru stock in Bali Strait had been overexploited, so that it threaten

  7. Impact of secondary hard substrate on the distribution and abundance of Aurelia aurita in the western Baltic Sea.

    Science.gov (United States)

    Janßen, H; Augustin, C B; Hinrichsen, H H; Kube, S

    2013-10-15

    This study assessed the impact of secondary hard substrate, as being introduced into marine ecosystems by the establishment of wind farm pillars, on the occurrence and distribution of the moon jelly Aurelia aurita in the southwestern Baltic Sea. A two-year data sampling was conducted with removable settlement plates to assess the distribution and population development of the scyphozoan polyps. The data collected from these samples were used to set up a model with Lagrangian particle technique. The results confirm that anthropogenic created hard substrate (e.g. offshore wind farms) has the potential to increase the abundance of the A. aurita population. The distribution of wind farm borne jellyfish along Danish, German and Polish coasts indicates conflicts with further sectors, mainly energy and tourism. Copyright © 2013 Elsevier Ltd. All rights reserved.

  8. Induction of segmentation in polyps of Aurelia aurita (Scyphozoa, Cnidaria) into medusae and formation of mirror-image medusa anlagen.

    Science.gov (United States)

    Kroiher, M; Siefker, B; Berking, S

    2000-08-01

    Polyps of Aurelia aurita can transform into several medusae (jellyfish) in a process of sequential subdivision. During this transformation, two processes take place which are well known to play a key role in the formation of various higher metazoa: segmentation and metamorphosis. In order to compare these processes in bilaterians and cnidarians we studied the control and the kinetics of these processes in Aurelia aurita. Segmentation and metamorphosis visibly start at the polyp's head and proceed down the body column but do not reach the basal disc. The small piece of polyp which remains will develop into a new polyp. The commitment to the medusa stage moves down the body column and precedes the visible onset of segmentation by about one day. Segmentation and metamorphosis can start at the cut surface of transversely cut body columns, leading to a mirror-image pattern of sequentially developing medusae.

  9. Improving the Quality of Sardine Fish Oil by Degumming Using Sodium Cholride Solution

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    Dian Prima Christiani Hulu

    2017-04-01

    Full Text Available The quality of fish oil is determined by some parameters such as primary and secondary grade oxidation, fatty acid profile and physical qualities which include viscosity, density, color and clarity. Sardine fish oil by-products of fish meal processing can be a source of polyunsaturated fatty acids (PUFAs, particularlyeicosapentaenoic acid (EPA and docosahexaenoic acid (DHA. Degumming is one of steps that can be carried out to improve the quality of sardine fish oil. The processing will help to reduce oxidation and eliminate the impurity of the oil that influence the quality of fish oil. The purpose of this study was to determine the effect of sodium chloride solution degumming on the quality of sardine fish oil to fulfill International Fish Oil Standard (IFOS. Fish oil purification was done by degumming of 5% H2O,  followedby sodium chloride solution with ratio of fish oil and sodium chloride solution were 1:1; 1:3, and 1:5. The sodium chloride solution concentrations of 5% and 8% for 20, 30 and 40 minutes. The next steps after degumming process were followed by alkali neutralization and bleached by absorbent. The best treatmentwas at  concentration of 5%  sodium chloride solution, ratio fish oil with sodium chloride solution 1:1 on a long time degumming processed 20 minutes. The best quality of fish oil, according to IFOS, was resulted from the treatments combination of free fatty acid 0.21±0.00%, peroxide value 0.43±0.06 mEq/kg, anisidin value 2.22±0.04 mEq/kg and total oxidation 3.11±0.14  mEq/kg.

  10. Effect of natural antioxidants on the quality of frozen sardine fillets (Sardina pilchardus

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    Rui Manuel Maneta Ganhão

    2014-06-01

    Full Text Available The use of new technologies / processes provides different benefits on seafood sector, namely by enhanced the shelf-life of fish products with high polyunsaturated fatty acid composition. This is particularly critical during some months of the year when fish (e.g. sardines are larger supply than demand by consumers. The aim of this study was to performed the optimization process of adding natural antioxidants on frozen sardine fillets and evaluated the effect of addition of two natural antioxidants on the lipid oxidation process during storage at -200C. To evaluate the effect of the addition of antioxidants in sardine fillets, the product matrix was chemical characterized, including by the evaluation of the fatty acid profile. The lipid oxidation process was followed by the primary products (peroxide value and secondary products (TBARS quantification, and also by the analysis of the sensory changes (instrumental assessment of color. Finally, it was done a hedonic preference test to know the opinion of consumers about the frozen fillets with and without antioxidant. The natural antioxidants used, tocopherol (54 mg/fillet and tocopherols (15 mg/fillet with rosemary extract (6.5 mg/fillet inhibited or retarded the oxidation over time of storage when compared with the control samples, that had advanced oxidation process. There was not statistically differences among the fillets treated with tocopherol in the presence and absence of rosemary extract. However, our laboratorial results shown slight tendency for the view that the antioxidant tocopherols and rosemary extract had a greater effect on the oxidative stability of fillets. This observation is in line with results of the hedonic test consumer preference. The use of natural antioxidants is an inexpensive solution which allows the complete use of products with less waste of fish products and with a great need for disposal. At the same time meets the consumer´s requirements.

  11. Relative role of life-history traits and historical factors in shaping genetic population structure of sardines (Sardina pilchardus

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    Zardoya Rafael

    2007-10-01

    Full Text Available Abstract Background Marine pelagic fishes exhibit rather complex patterns of genetic differentiation, which are the result of both historical processes and present day gene flow. Comparative multi-locus analyses based on both nuclear and mitochondrial genetic markers are probably the most efficient and informative approach to discerning the relative role of historical events and life-history traits in shaping genetic heterogeneity. The European sardine (Sardina pilchardus is a small pelagic fish with a relatively high migratory capability that is expected to show low levels of genetic differentiation among populations. Previous genetic studies based on meristic and mitochondrial control region haplotype frequency data supported the existence of two sardine subspecies (S. p. pilchardus and S. p. sardina. Results We investigated genetic structure of sardine among nine locations in the Atlantic Ocean and Mediterranean Sea using allelic size variation of eight specific microsatellite loci. Bayesian clustering and assignment tests, maximum likelihood estimates of migration rates, as well as classical genetic-variance-based methods (hierarchical AMOVA test and RST pairwise comparisons supported a single evolutionary unit for sardines. These analyses only detected weak but significant genetic differentiation, which followed an isolation-by-distance pattern according to Mantel test. Conclusion We suggest that the discordant genetic structuring patterns inferred based on mitochondrial and microsatellite data might indicate that the two different classes of molecular markers may be reflecting different and complementary aspects of the evolutionary history of sardine. Mitochondrial data might be reflecting past isolation of sardine populations into two distinct groupings during Pleistocene whereas microsatellite data reveal the existence of present day gene flow among populations, and a pattern of isolation by distance.

  12. Spatial distribution of sardine and anchovy early life stages along the eastern Adriatic Sea

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    Barbara Zorica

    2015-10-01

    Full Text Available Despite long-term investigations of the Adriatic Sea in general and extensive research on sardine and anchovy as ecologically and economically important fish species, knowledge concerning their spawning ecology, precisely their possible spawning grounds, is still incomplete. Therefore, two scientific surveys (January, July 2012 were performed, as target species spawn during different periods of the year (sardine - October to April (Sinovčić et al., 2007 and anchovy - April to September (Zorica et al., 2013. Throughout these surveys, standard vertical plankton tows were made during the daylight at 72 stations in January and 60 stations in July using a WP2 sampler (mouth opening, 0.255 m2; mesh size, 0.200 mm. WP2 net was put vertically down during the calm sea to a depth of 100 m or to 5 m above the seabed in relatively shallow marine area (less than 100 m. Plankton samples were preserved in 4% buffered formalin and transported to the laboratory where they were analysed. Throughout ichtyoplankton analysis of samples collected during the winter survey a total of 506 sardine eggs and 348 larvae were sorted out. The mean sardine egg and larval abundance in the analysed period at positive stations was 57.8 +/- 77.4 eggs/m2 and 29 +/- 25.85 larvae/m2, with peaks of 396 eggs/m2 and 108 larvae/m2 at positive sampling stations. Concerning the summer survey and anchovy early life stages from obtained samples 1489 anchovy eggs and 1036 larvae were isolated. Average abundance of anchovy eggs and larvae was 145.26 +/- 201.27eggs/m2 and 115.11+/- 162.32 larvae/m2, respectively. The highest values of mentioned parameters at positive stations were 800 eggs/m2 and 952 larvae/m2. According to obtained results and their processing within Ocean Data View (odv.awi.de, revile that both species during 2012 spawn above the whole continental shelf and the areas of higher early life stages abundance overlap.

  13. Análise econômica da produção da sardinha na região sudeste do Brasil Economical analysis of the sardine yield in southeastern Brazil

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    Yasunobu Matsuura

    1981-06-01

    Full Text Available A surplus-yield model of Schaefer (1954; 1957 was used to study a maximum sustainable yield of the Brazilian sardine. Applying the prices of sardine and operation cost, an economical yield of sardine was discussed. The maximum sustainable yield of sardine was estimated on 173 thousands metric tons and the optimum fishing effort was 28,292 sets of net. With the operation cost of Cr$ 71,040.00 per cruise, the price of sardine has to be higher than Cr$ 10,00/kg.

  14. Offshore dispersion of ephyrae and medusae of Aurelia aurita s.l. (Cnidaria: Scyphozoa) from port enclosures: Physical and biological factors

    Science.gov (United States)

    Makabe, Ryosuke; Takeoka, Hidetaka; Uye, Shin-ichi

    2015-12-01

    Recurrent outbreaks of the common jellyfish Aurelia aurita s.l. have been increasingly significant, particularly in human perturbed coastal waters, where numerous artificial constructions increase suitable habitat for polyp populations. We examined the spatiotemporal dispersion process in 6 ports of ephyrae of A. aurita after release from strobilating polyps, to offshore waters of northern Harima Nada (eutrophic eastern Inland Sea of Japan) from January to May 2010. Almost exclusive occurrence of the ephyra stage in the ports demonstrated that their seeding polyps reside in the port enclosures, and liberated ephyrae are rapidly exported offshore by tidal water exchange. Post-ephyra stages occurred primarily outside the ports, and their age increased gradually offshore, ca. up to 9 km off the ports, and the pattern of age increase could be simulated by a simple diffusion model. However, there was an abrupt decline in A. aurita density beyond ca. 3 km off the shore, where jellyfish-eating Chrysaora pacifica medusae were prevalent. We conclude that physical forces are primarily responsible for offshore dispersion of A. aurita, and a biological factor, i.e. predation by C. pacifica, jointly affects the distribution pattern of A. aurita.

  15. CARACTERIZAÇÃO QUÍMICA E ACEITABILIDADE DA SARDINHA (SARDINELLA BRASILIENSIS EM CONSERVA ADICIONADA DE MOLHO COM TOMATE

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    Janise Pedroso COLEMBERGUE

    2011-04-01

    Full Text Available O pescado é um alimento rico em proteínas, minerais, vitaminas e possui fácil digestão. A procura por alimentos prontos para o consumo, como pescado em conserva, ainda é uma opção interessante para o consumidor, devido ao tempo reduzido de preparação. As características sensoriais estão entre os principais determinantes na aquisição, consumo, aceitação e preferência dos produtos alimentícios por diferentes faixas etárias. A aceitabilidade da sardinha (Sardinella brasiliensis em conserva de molho de tomate foi avaliada por 50 provadores não treinados em ambiente laboratorial através de uma fi cha com escala hedônica de nove pontos. Além disso, a determinação da composição química foi realizada e comparada com os valores declarados no rótulo do produto. A aceitação obtida para este tipo de conserva foi de 84% e a média de pontuação de 7.56, sendo esta caracterizada entre “gostei” e “gostei muito”, observou-se ainda que 54% dos julgadores possuíam hábitos de consumir pescado em conserva. A composição química da sardinha em conserva obtida foi de 62,44±0,33% de umidade, 2,7±0,22% de resíduo mineral fi xo, 19,35±1,05% de proteínas e 15,11±0,6% de lipídios totais, apresentando-se de acordo com a rotulagem, exceto para o teor de lipídio. Este estudo permitiu observar a grande aceitabilidade da sardinha (Sardinella brasiliensis em conserva de molho com tomate, mesmo entre os julgadores que não possuíam o hábito de consumi-lo.

  16. Mixed layer depth variations in the Kuroshio Extension in relation to Japanese sardine

    Science.gov (United States)

    Yasuda, I.; Nishikawa, H.; Itoh, S.

    2008-12-01

    Mixed layer depths in and south of the Kuroshio Extension changed from deep to shallow states in the late- 1980s and early-1990s. This change corresponded to the collapse of Japanese sardine (Sardinops melanostictus). This mixed layer shoaling was accompanied by lower temperature in 200-400m depths and higher temperature near the surface. Wintertime field survey in 2006 (Hakuho-maru KH06-1 cruise) demonstrated that late winter maximum mixed layer depth reach deeper with the greater isothermal depth of 14-15degC and higher temperature in 300-400m depth. High-resolution ocean model hindcast data suggests that the accelerated near-surface Kuroshio/Kuroshio Extension associated with the elevated sea-surface height anomaly enhanced the heat transport near the surface. This greater heat advection near the surface overrides the cooler subtropical mode water that was created in the previous years possibly causes the shallower winter mixed layer and collapse of the Japanese sardine.

  17. Spatially-explicit bioenergetics of Pacific sardine in the Southern California Bight: are mesoscale eddies areas of exceptional prerecruit production?

    Science.gov (United States)

    Logerwell, Elizabeth A.; Lavaniegos, Bertha; Smith, Paul E.

    Previous research shows that offshore mesoscale eddies in the Southern California Bight region are areas where sardine larval abundance is significantly increased relative to inshore, slope and surrounding offshore waters. In order for mesoscale eddies to be a mechanism linking climate and sardine population variability they must be areas of exceptional prerecruit production. Temperature and prey data from various Southern California Bight (SCB) habitats, including offshore eddies, were applied to a spatially-explicit bioenergetic model which predicts sardine prerecruit growth potential. Growth potential was similar in inshore, slope, and eddy regions (11% and 12% day -1), and was lower in the offshore region, 9% day -1. To estimate production in eddy and non-eddy habitats, growth potential was multiplied by habitat-specific estimates of sardine larval biomass from at-sea surveys. A production index, a measure of potential production resulting from individual growth rate potential and local abundance, was greater in the model cyclonic eddy than in all other regions by more than an order of magnitude. In fact, the production index in the eddy was four times greater than in all other regions combined.

  18. Effect of controlled lactic acid bacterial fermentation on the microbiological and chemical qualities of Moroccan sardines (Sardina pilchardus).

    Science.gov (United States)

    Ndaw, A; Zinedine, A; Faid, M; Bouseta, A

    2008-09-01

    Lactic acid bacteria (LAB) strains were assayed for the conservation of fresh sardine "Sardina pilchardus". Lactobacillus delbrueckii subsp. delbrueckii was used for inoculation of sardine fillets in a solution of NaCl (5%, w/w) and glucose (4%, w/w) concentration in water. Microbial counts including Standard Plate Count (SPC), LAB, yeasts, coliforms, Salmonella, staphylococci and Clostridium were followed during two weeks of storage at 30 degrees C. Determinations of chemical parameters including pH, dry matter, fat, ash, total nitrogen (NT), total volatile basic nitrogen (TVBN) and trimethylamine (TMA) were carried out under the same conditions. Chemical determinations showed a net pH decrease from an initial value of 6.05 in raw sardine fillets to 4.3 after 16 days of fermentation. Increases in TMA content and TBVN were observed. Microbiological control showed that LAB counts reached a level up to 3.10(9) cfu/g after 4 days of fermentation. After two weeks, fermented fish was free of coliforms and Salmonella. The inhibition of pathogenic microflora including staphylococci and Clostridium was also observed. The results indicated that controlled LAB fermentation could be used as a successful process for biopreservation of sardines produced in huge quantities in Morocco.

  19. Dietary sardine protein lowers insulin resistance, leptin and TNF-α and beneficially affects adipose tissue oxidative stress in rats with fructose-induced metabolic syndrome.

    Science.gov (United States)

    Madani, Zohra; Louchami, Karim; Sener, Abdullah; Malaisse, Willy J; Ait Yahia, Dalila

    2012-02-01

    The present study aims at exploring the effects of sardine protein on insulin resistance, plasma lipid profile, as well as oxidative and inflammatory status in rats with fructose-induced metabolic syndrome. Rats were fed sardine protein (S) or casein (C) diets supplemented or not with high-fructose (HF) for 2 months. Rats fed the HF diets had greater body weight and adiposity and lower food intake as compared to control rats. Increased plasma glucose, insulin, HbA1C, triacylglycerols, free fatty acids and impaired glucose tolerance and insulin resistance was observed in HF-fed rats. Moreover, a decline in adipose tissues antioxidant status and a rise in lipid peroxidation and plasma TNF-α and fibrinogen were noted. Rats fed sardine protein diets exhibited lower food intake and fat mass than those fed casein diets. Sardine protein diets diminished plasma insulin and insulin resistance. Plasma triacylglycerol and free fatty acids were also lower, while those of α-tocopherol, taurine and calcium were enhanced as compared to casein diets. Moreover, S-HF diet significantly decreased plasma glucose and HbA1C. Sardine protein consumption lowered hydroperoxide levels in perirenal and brown adipose tissues. The S-HF diet, as compared to C-HF diet decreased epididymal hydroperoxides. Feeding sardine protein diets decreased brown adipose tissue carbonyls and increased glutathione peroxidase activity. Perirenal and epididymal superoxide dismutase and catalase activities and brown catalase activity were significantly greater in S-HF group than in C-HF group. Sardine protein diets also prevented hyperleptinemia and reduced inflammatory status in comparison with rats fed casein diets. Taken together, these results support the beneficial effect of sardine protein in fructose-induced metabolic syndrome on such variables as hyperglycemia, insulin resistance, hyperlipidemia and oxidative and inflammatory status, suggesting the possible use of sardine protein as a protective

  20. ENRIQUECIMENTO AMBIENTAL X COMPORTAMENTO EM CATIVEIRO DE CALLITHRIX AURITA (É. Geoffroy, 1812

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    Marcellus Pereira Souza

    2017-02-01

    Full Text Available Técnicas de enriquecimento ambiental foram utilizadas para avaliação dos comportamentos em cativeiro de um grupo de três indivíduos, duas fêmeas e um macho de Callithrix aurita (sagui-da-serra-escuro, cativos do Mantenedor de Fauna Silvestre da UNIVAP, São José dos Campos, SP. Foram implantados seis diferentes enriquecimentos alimentares e observações com duração de uma hora pelo método “focal sampling grupal” no período de agosto a novembro de 2015. Os comportamentos categorizados, inicialmente, eram Alimentação, Alerta, Comunicação, Social, Manutenção e Território. A categoria Cuidado Parental foi adicionada após o nascimento de dois filhotes. Percebeu-se que Alimentação e Alerta foram as categorias mais evidentes, que Cuidado Parental apareceu equilibrada entre os membros e as expressões de comportamentos típicos foram significantes para concluir que enriquecimentos ambientais melhoram o bem-estar animal de animal em cativeiro.

  1. Artificial substrates preference for proliferation and immigration in Aurelia aurita (s. l.) polyps

    Science.gov (United States)

    Feng, Song; Lin, Jianing; Sun, Song; Zhang, Fang

    2017-01-01

    The increasing amounts of artificial marine substrates, in many parts of the world have been proposed as a potential driver of Aurelia spp. blooms, on account of providing extra habitats for the settlement and the proliferation of the benthic stage (polyps). Previous experiments have mainly focused on the substrate choices of Aurelia spp. planulae. However, substrate preferences for the proliferation and immigration of polyps have not been reported. We monitored the propagation and immigration of Aurelia aurita (s. l.) polyps on two natural and nine artificial substrates at constant temperature (20±0.5°C) and salinity (30±0.5) in beakers and a glass aquarium in the laboratory, respectively. The results showed that, among artificial substrates, the highest number for polyp proliferation and immigration was found on nets, rigid polyvinyl chloride plates (RPVC), and wood. The lowest density of polyps was present on iron plates. Among natural substrates, the asexual reproduction rate of polyps on Patinopecten yessoensis (Jay, 1857) shells was significantly higher than Azumapecten farreri (Jones & Preston, 1904). On the account of the distinction in the roughness, chemical properties and biofilms of these material surfaces, bare artificial or natural substrates discriminatively affect the proliferation and the immigration of Aurelia spp. polyps at laboratory. These observations suggest that, even in the natural environment, different materials and texture may influence the composition and the abundance of the fouling communities and the assemblages of polyps and, indirectly, have effects on the amounts of released medusae.

  2. New Aspidoderidae species parasite of Didelphis aurita (Mammalia: Didelphidae): a light and scanning electron microscopy approach.

    Science.gov (United States)

    Chagas-Moutinho, V A; Sant'anna, V; Oliveira-Menezes, A; De Souza, W

    2014-02-01

    Nematodes of the family Aspidoderidae (Nematoda: Heterakoidea) Skrjabin and Schikobalova, 1947, are widely distributed in the Americas. The family Aspidoderidae includes the subfamilies Aspidoderinae Skrjabin and Schikobalova, 1947, and Lauroiinae Skrjabin and Schikobalova, 1951. These two subfamilies are delineated by the presence or absence of cephalic cordons at the anterior region. The nematodes in the subfamily Aspidoderinae, which includes the genus AspidoderaRailliet and Henry, 1912, are represented by nematodes with anterior cephalic cordons at the anterior end. The nematodes of the genus AspidoderaRailliet and Henry, 1912, are found in the cecum and large intestine of mammals of the orders Edentata, Marsupialia and Rodentia. Species within this genus have many morphological similarities. The use of scanning electron microscopy allows the specific characterization of the species within this genus. In the present work, we describe a new species of Aspidodera parasite of the large intestine of Didelphis aurita (Mammalia: Didelphidae) Wied-Neuwied, 1826, collected from Cachoeiras de Macacu, Rio de Janeiro. The combination of light and scanning electron microscopy allowed us a detailed analysis of this nematode.

  3. Plankton Dynamics and Aurelia auritaProduction in Two Contrasting Ecosystems: Comparisons and Consequences

    Science.gov (United States)

    Lucas, C. H.; Hirst, A. G.; Williams, J. A.

    1997-08-01

    The population dynamics and production of the Scyphomedusa Aurelia aurita(Lam.) were examined within two contrasting ecosystems on the south coast of England; Southampton Water, an open productive estuary, and Horsea Lake, an enclosed, brackish, man-made body of water. Annual A. auritaproduction in Southampton Water ranged between 31 and 58 mg C m -3year -1in different years (1990-94); while at Horsea Lake, annual production was 76 mg C m -3year -1in 1994. The potential impact of A. auritaon the mesozooplankton communities was estimated through measurements of in situsecondary production and transfer efficiency between the two populations. Using literature-based estimates for mesozooplankton production, the annual transfer efficiency between the mesozooplankton and A. auritapopulations in Southampton Water ranged between 9 and 26% in different years, and in Horsea Lake was 73% in 1994. These data imply that the abundant A. auritapopulation may have a significant impact on the mesozooplankton community of Horsea Lake, and in turn, the very small A. auritasizes observed indicate a severely food limited population. The ecological role of A. auritain Horsea Lake is discussed in view of the very low chl aand mesozooplankton numbers present.

  4. Functional morphology and fluid interactions during early development of the scyphomedusa Aurelia aurita.

    Science.gov (United States)

    Feitl, K E; Millett, A F; Colin, S P; Dabiri, J O; Costello, J H

    2009-12-01

    Scyphomedusae undergo a predictable ontogenetic transition from a conserved, universal larval form to a diverse array of adult morphologies. This transition entails a change in bell morphology from a highly discontinuous ephyral form, with deep clefts separating eight discrete lappets, to a continuous solid umbrella-like adult form. We used a combination of kinematic, modeling, and flow visualization techniques to examine the function of the medusan bell throughout the developmental changes of the scyphomedusa Aurelia aurita. We found that flow around swimming ephyrae and their lappets was relatively viscous (1 < Re < 10) and, as a result, ephyral lappets were surrounded by thick, overlapping boundary layers that occluded flow through the gaps between lappets. As medusae grew, their fluid environment became increasingly influenced by inertial forces (10 < Re < 10,000) and, simultaneously, clefts between the lappets were replaced by organic tissue. Hence, although the bell undergoes a structural transition from discontinuous (lappets with gaps) to continuous (solid bell) surfaces during development, all developmental stages maintain functionally continuous paddling surfaces. This developmental pattern enables ephyrae to efficiently allocate tissue to bell diameter increase via lappet growth, while minimizing tissue allocation to inter-lappet spaces that maintain paddle function due to boundary layer overlap.

  5. Recombinant expression and solution structure of antimicrobial peptide aurelin from jellyfish Aurelia aurita.

    Science.gov (United States)

    Shenkarev, Zakhar O; Panteleev, Pavel V; Balandin, Sergey V; Gizatullina, Albina K; Altukhov, Dmitry A; Finkina, Ekaterina I; Kokryakov, Vladimir N; Arseniev, Alexander S; Ovchinnikova, Tatiana V

    2012-12-07

    Aurelin is a 40-residue cationic antimicrobial peptide isolated from the mezoglea of a scyphoid jellyfish Aurelia aurita. Aurelin and its (15)N-labeled analogue were overexpressed in Escherichia coli and purified. Antimicrobial activity of the recombinant peptide was examined, and its spatial structure was studied by NMR spectroscopy. Aurelin represents a compact globule, enclosing one 3(10)-helix and two α-helical regions cross-linked by three disulfide bonds. The peptide binds to anionic lipid (POPC/DOPG, 3:1) vesicles even at physiological salt concentration, it does not interact with zwitterionic (POPC) vesicles and interacts with the DPC micelle surface with moderate affinity via two α-helical regions. Although aurelin shows structural homology to the BgK and ShK toxins of sea anemones, its surface does not possess the "functional dyad" required for the high-affinity interaction with the K(+)-channels. The obtained data permit to correlate the modest antibacterial properties and membrane activity of aurelin. Copyright © 2012 Elsevier Inc. All rights reserved.

  6. Mesogleal cells of the jellyfish Aurelia aurita are involved in the formation of mesogleal fibres.

    Science.gov (United States)

    Shaposhnikova, Tatiana; Matveev, Ivan; Napara, Tatiana; Podgornaya, Olga

    2005-11-01

    The extracellular matrix of the jellyfish Aurelia aurita (Scyphozoa, Cnidaria), known as the mesoglea, is populated by numerous mesogleal cells (Mc). We determined the pattern of the Mc and the mesoglea, raised polyclonal antibodies (RA47) against the major mesogleal protein pA47 (47 kDa) and checked their specificity. In the mesoglea, RA47 stains pA47 itself. In immunoblots of Mc, RA47 stains bands of 120 kDa and 80 kDa; weaker staining is observed at pA47. The same staining pattern is seen on blots of jellyfish epidermal cells and of whole Hydra (Hydrozoa) or isolated mesoglea of Hydra. Our data indicate that pA47 is synthesized by Mc and epidermal cells as high molecular precursors. Using immunostaining techniques, we showed Mc to be involved in the formation of mesogleal non-collagenous (called "elastic" in classic morphological studies) fibres. The biochemical and morphological data suggest that Mc originate from the epidermis.

  7. Effect of different types of coatings on texture and nutritional properties of canned Portuguese sardines (Sardina pilchardus

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    Rita Pinheiro

    2014-06-01

    Full Text Available Canned foods have a long history of success and are likely to remain popular for the foreseeable future owing to their convenience, long shelf life and economy. Interest in fish consumption has increased in recent years due to the wide range of its health benefits. In addition to fresh product, canned fish enables a delayed consumption of this appreciated kind of food. Among the different types of fish, in Portugal, the most used in canning manufacture are sardines, tuna, anchovies, mackerels. Vegetable oils and tomato sauce are usually adopted as coatings (liquid medium. In fact, oil has a preserving effect and contributes to make the product more palatable. Its protective action lies in the ability to insulate products from air, rather than having an active bacteriostatic or bactericidal action. Among the different types of coatings the most commonly used in canning are: olive oil, seed oils and different sauces, such as tomato sauce. Tomato sauce has also vegetable oil on its formulation. The amount of coating substance can affect the nutritional composition as well as texture properties of the sardines. The aim of this work was to investigate the effect of different types of coatings on texture and nutritional properties of whole, skinless and boneless canned Portuguese Sardines (Sardina pilchardus. An experimental investigation was carried out to assess the protein, lipid, carbohydrates and ash content, texture and colour profile of canned sardine with different kinds of coatings: olive oil, sunflower oil and tomato sauce. The obtained results showed that protein and carbohydrates content were not significantly influenced by the coating used, varying between 5%-8% and13%-18%, respectively. For the moisture content it was found that the samples with tomato sauce were 2.2-fold higher than the samples with sunflower oil, and 1.3-fold higher than the samples with olive oil, independently of being whole, skinless or boneless sardine. As

  8. Interannual Changes in Biomass Affect the Spatial Aggregations of Anchovy and Sardine as Evidenced by Geostatistical and Spatial Indicators.

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    Marco Barra

    Full Text Available Geostatistical techniques were applied and a series of spatial indicators were calculated (occupation, aggregation, location, dispersion, spatial autocorrelation and overlap to characterize the spatial distributions of European anchovy and sardine during summer. Two ecosystems were compared for this purpose, both located in the Mediterranean Sea: the Strait of Sicily (upwelling area and the North Aegean Sea (continental shelf area, influenced by freshwater. Although the biomass of anchovy and sardine presented high interannual variability in both areas, the location of the centres of gravity and the main spatial patches of their populations were very similar between years. The size of the patches representing the dominant part of the abundance (80% was mostly ecosystem- and species-specific. Occupation (area of presence appears to be shaped by the extent of suitable habitats in each ecosystem whereas aggregation patterns (how the populations are distributed within the area of presence were species-specific and related to levels of population biomass. In the upwelling area, both species showed consistently higher occupation values compared to the continental shelf area. Certain characteristics of the spatial distribution of sardine (e.g. spreading area, overlapping with anchovy differed substantially between the two ecosystems. Principal component analysis of geostatistical and spatial indicators revealed that biomass was significantly related to a suite of, rather than single, spatial indicators. At the spatial scale of our study, strong correlations emerged between biomass and the first principal component axis with highly positive loadings for occupation, aggregation and patchiness, independently of species and ecosystem. Overlapping between anchovy and sardine increased with the increase of sardine biomass but decreased with the increase of anchovy. This contrasting pattern was attributed to the location of the respective major patches

  9. Clearance rates of ephyrae and small medusae of the common jellyfish Aurelia aurita offered different types of prey

    Science.gov (United States)

    Riisgård, Hans Ulrik; Madsen, Caroline V.

    2011-01-01

    Prey selection and knowledge of the amounts of water processed by the early stages of the common jellyfish Aurelia aurita may at certain times of the year be crucial for understanding the plankton dynamics in marine ecosystems with mass occurrences of this jellyfish. In the present study we used two different methods ("clearance method" and "ingestion-rate method") to estimate the amount of water cleared per unit of time of different types and sizes of prey organisms offered to A. aurita ephyrae and small medusae. The mean clearance rates of medusae, estimated with Artemia sp. nauplii as prey by both methods, agreed well, namely 3.8 ± 1.4 l h - 1 by the clearance method and 3.2 ± 1.1 l h - 1 by the ingestion-rate method. Both methods showed that copepods (nauplii and adults) and mussel veligers are captured with considerably lower efficiency, 22 to 37% and 14 to 30%, respectively, than Artemia salina nauplii. By contrast, the water processing rates of ephyrae measured by the clearance method with A. salina nauplii as prey were 3 to 5 times lower than those measured by the ingestion-rate method. This indicates that the prerequisite of full mixing for using the clearance method may not have been fulfilled in the ephyrae experiments. The study demonstrates that the predation impact of the young stages of A. aurita is strongly dependent on its developmental stage (ephyra versus medusa), and the types and sizes of prey organisms. The estimated prey-digestion time of 1.3 h in a steady-state feeding experiment with constant prey concentration supports the reliability of the ingestion-rate method, which eliminates the negative "container effects" of the clearance method, and it seems to be useful in future jellyfish studies, especially on small species/younger stages in which both type and number of prey can be easily and precisely assessed.

  10. Exploitation des larves de sardine Limnothrissa miodon au lac Kivu (R.D.C. : danger potentiel ?

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    Kaningini, M.

    1997-01-01

    Full Text Available Larva's Catch of Sardine Limnothrissa miodon in Lake Kivu (RD Congo : Potential Danger ?. Commercial frog farms exist in several countries. It seems hopeless believing that frog leg consumption can be prohibited, and the solution is probably a very strict control of the origin of the legs sold and imported. Uncontrolled hunting-poaching of frogs should be replaced by sustainable and rational breeding of frogs for sale. A large frog farm located near Singapore is described with the infrastructure and the management of the production. The two species bred are the American and the Asian bull-frogs Rana catesbeiana and R. tigerina. It is also mentioned that appropriate slaughtering techniques exist for frogs based on same guidelines as for conventional farm animals.

  11. The use of pelagic fish as proxies of environmental contamination: a case study with sardine populations

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    Bruno Silva Nunes

    2015-12-01

    Full Text Available The use of bioindicators to analyze marine ecosystems contamination is often made difficult due to the absence of appropriate species. Pelagic fish species, captured by commercial fishing fleets around the world, can be successfully used to assess contaminant levels, by determining their body burden in specific compounds. However the study of biological responses elicited by such compounds, through the analysis of biomarkers, is highly dependent on the physiological and reproductive status of the organisms. Such confounding factors elicit seasonal fluctuations that difficult the extrapolation of data. Sardine (Sardina pilchardus is a marine species common in the North Atlantic Ocean, being easily available through commercial fisheries. The present work intended to explore the potential of this species in biomonitoring studies, by simultaneously using enzymatic biomarkers and condition indices determined in fish landed in three commercial harbors along the west coast of Portugal. This strategy allowed devising spatial and temporal patterns in the sardine metapopulation. Results evidenced significant variability in both biochemical and physiological profiles of the fish, which were coherent among all sampling sites. Throughout the year, large seasonal differences for most markers were reported, which were strongly linked to the reproductive cycle and its physiological consequences (acquisition of energy, mobilization of energy reserves, etc.. It was possible to conclude that seasonality acts as a strong factor underlying chronological physiological adaptations, influencing biochemical markers that are usually employed as indicators of contamination. These effects can limit the usefulness of such a biomarker approach unless seasonality is not accounted for, and if no background values are known from previous studies. In this sense, studies such as this are pivotal to establish a baseline for biomonitoring studies. Also, despite the difficulty in

  12. The ontogenetic scaling of hydrodynamics and swimming performance in jellyfish (Aurelia aurita).

    Science.gov (United States)

    McHenry, Matthew J; Jed, Jason

    2003-11-01

    It is not well understood how ontogenetic changes in the motion and morphology of aquatic animals influence the performance of swimming. The goals of the present study were to understand how changes in size, shape and behavior affect the hydrodynamics of jet propulsion in the jellyfish Aurelia aurita and to explore how such changes affect the ontogenetic scaling of swimming speed and cost of transport. We measured the kinematics of jellyfish swimming from video recordings and simulated the hydrodynamics of swimming with two computational models that calculated thrust generation by paddle and jet mechanisms. Our results suggest that thrust is generated primarily by jetting and that there is negligible thrust generation by paddling. We examined how fluid forces scaled with body mass using the jet model. Despite an ontogenetic increase in the range of motion by the bell diameter and a decrease in the height-to-diameter ratio, we found that thrust and acceleration reaction scaled with body mass as predicted by kinematic similarity. However, jellyfish decreased their pulse frequency with growth, and speed consequently scaled at a lower exponential rate than predicted by kinematic similarity. Model simulations suggest that the allometric growth in Aurelia results in swimming that is slower, but more energetically economical, than isometric growth with a prolate bell shape. The decrease in pulse frequency over ontogeny allows large Aurelia medusae to avoid a high cost of transport but generates slower swimming than if they maintained a high pulse frequency. Our findings suggest that ontogenetic change in the height-to-diameter ratio and pulse frequency of Aurelia results in swimming that is relatively moderate in speed but is energetically economical.

  13. Microbacterium aureliae sp. nov., a novel actinobacterium isolated from Aurelia aurita, the moon jellyfish.

    Science.gov (United States)

    Kaur, Gurwinder; Mual, Parveen; Kumar, Narender; Verma, Ashish; Kumar, Anand; Krishnamurthi, Srinivasan; Mayilraj, Shanmugam

    2016-11-01

    The taxonomic position of a lemon-yellow-pigmented actinobacterium, strain JF-6T, isolated from Aurelia aurita, the moon jellyfish, collected from the Bay of Bengal coast, Kanyakumari, India, was determined using a polyphasic approach. The strain had phenotypic and chemotaxonomic properties that were consistent with its classification in the genus Microbacterium. Alignment of the 16S rRNA gene sequence of strain JF-6T with sequences from Microbacterium arthrosphaerae CC-VM-YT, Microbacterium yannicii G72T, Microbacterium trichothecenolyticum IFO 15077T, Microbacterium flavescens DSM 20643T, Microbacterium insulae DS-66T, Microbacterium resistens DMMZ 1710T and Microbacterium thalassium IFO 16060T revealed similarities of 98.95, 98.76, 98.43, 98.41, 98.41, 98.26 and 98.22 %, respectively. However, the levels of DNA-DNA relatedness with its closest phylogenetic neighbours confirmed that it represents a novel species within the genus. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and an unknown glycolipid. The major menaquinones detected for strain JF-6T were MK-13 and MK-12. The diamino acid in the cell-wall peptidoglycan was ornithine and the peptidoglycan was type B2β (Glu/Hyg-Gly-d-Orn). The DNA G+C content was 69.4 mol%. Based on these differences, strain JF-6T (=MTCC 11843T=JCM 30060T=KCTC 39828T) should be classified as the type strain of a novel species of Microbacterium, for which the name Microbacterium aureliae sp. nov. is proposed.

  14. Transcriptome profiling of the dynamic life cycle of the scypohozoan jellyfish Aurelia aurita.

    Science.gov (United States)

    Brekhman, Vera; Malik, Assaf; Haas, Brian; Sher, Noa; Lotan, Tamar

    2015-02-14

    The moon jellyfish Aurelia aurita is a widespread scyphozoan species that forms large seasonal blooms. Here we provide the first comprehensive view of the entire complex life of the Aurelia Red Sea strain by employing transcriptomic profiling of each stage from planula to mature medusa. A de novo transcriptome was assembled from Illumina RNA-Seq data generated from six stages throughout the Aurelia life cycle. Transcript expression profiling yielded clusters of annotated transcripts with functions related to each specific life-cycle stage. Free-swimming planulae were found highly enriched for functions related to cilia and microtubules, and the drastic morphogenetic process undergone by the planula while establishing the future body of the polyp may be mediated by specifically expressed Wnt ligands. Specific transcripts related to sensory functions were found in the strobila and the ephyra, whereas extracellular matrix functions were enriched in the medusa due to high expression of transcripts such as collagen, fibrillin and laminin, presumably involved in mesoglea development. The CL390-like gene, suggested to act as a strobilation hormone, was also highly expressed in the advanced strobila of the Red Sea species, and in the medusa stage we identified betaine-homocysteine methyltransferase, an enzyme that may play an important part in maintaining equilibrium of the medusa's bell. Finally, we identified the transcription factors participating in the Aurelia life-cycle and found that 70% of these 487 identified transcription factors were expressed in a developmental-stage-specific manner. This study provides the first scyphozoan transcriptome covering the entire developmental trajectory of the life cycle of Aurelia. It highlights the importance of numerous stage-specific transcription factors in driving morphological and functional changes throughout this complex metamorphosis, and is expected to be a valuable resource to the community.

  15. Recombinant expression and solution structure of antimicrobial peptide aurelin from jellyfish Aurelia aurita

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    Shenkarev, Zakhar O.; Panteleev, Pavel V.; Balandin, Sergey V. [Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str., 16/10, 117997 Moscow (Russian Federation); Gizatullina, Albina K.; Altukhov, Dmitry A. [Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str., 16/10, 117997 Moscow (Russian Federation); Moscow Institute of Physics and Technology (State University), Department of Physicochemical Biology and Biotechnology, Institutskii per., 9, 141700 Dolgoprudny, Moscow Region (Russian Federation); Finkina, Ekaterina I. [Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str., 16/10, 117997 Moscow (Russian Federation); Kokryakov, Vladimir N. [Institute of Experimental Medicine, Russian Academy of Medical Sciences, Academica Pavlova str., 12, 197376 Saint-Petersburg (Russian Federation); Arseniev, Alexander S. [Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str., 16/10, 117997 Moscow (Russian Federation); Moscow Institute of Physics and Technology (State University), Department of Physicochemical Biology and Biotechnology, Institutskii per., 9, 141700 Dolgoprudny, Moscow Region (Russian Federation); Ovchinnikova, Tatiana V., E-mail: ovch@ibch.ru [Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya str., 16/10, 117997 Moscow (Russian Federation); Moscow Institute of Physics and Technology (State University), Department of Physicochemical Biology and Biotechnology, Institutskii per., 9, 141700 Dolgoprudny, Moscow Region (Russian Federation)

    2012-12-07

    Highlights: Black-Right-Pointing-Pointer Aurelin was overexpressed in Escherichia coli, and its spatial structure was studied by NMR. Black-Right-Pointing-Pointer Aurelin compact structure encloses helical regions cross-linked by three disulfide bonds. Black-Right-Pointing-Pointer Aurelin shows structural homology to the BgK and ShK toxins of sea anemones. Black-Right-Pointing-Pointer Aurelin binds to the anionic lipid vesicles, but does not interact with zwitterionic ones. Black-Right-Pointing-Pointer Aurelin binds to DPC micelle surface with moderate affinity via two helical regions. -- Abstract: Aurelin is a 40-residue cationic antimicrobial peptide isolated from the mezoglea of a scyphoid jellyfish Aurelia aurita. Aurelin and its {sup 15}N-labeled analogue were overexpressed in Escherichia coli and purified. Antimicrobial activity of the recombinant peptide was examined, and its spatial structure was studied by NMR spectroscopy. Aurelin represents a compact globule, enclosing one 3{sub 10}-helix and two {alpha}-helical regions cross-linked by three disulfide bonds. The peptide binds to anionic lipid (POPC/DOPG, 3:1) vesicles even at physiological salt concentration, it does not interact with zwitterionic (POPC) vesicles and interacts with the DPC micelle surface with moderate affinity via two {alpha}-helical regions. Although aurelin shows structural homology to the BgK and ShK toxins of sea anemones, its surface does not possess the 'functional dyad' required for the high-affinity interaction with the K{sup +}-channels. The obtained data permit to correlate the modest antibacterial properties and membrane activity of aurelin.

  16. [Morphodynamics of the contract plate in the course of oocyte maturation in the scyphozoan Aurelia aurita (Cnidaria: Semaeostomae)].

    Science.gov (United States)

    Adonin, L S; Podgornaia, O I; Shaposhnikova, T G

    2012-01-01

    The structure forming in the area of contact between the oocyte and the germinal epithelium in the course of oocyte maturation of the scyphozoan Aurelia aurita is termed the contact plate. This study traces the successive stages of contact plate formation in the course of oocyte maturation at the light microscopic and ultrastructural levels. At early stages ofoocyte development, the appearance of granules is observed in the peripheral cytoplasm of the oocyte; these granules accumulate at the pole, which retains its connection with the germinal epithelium of the gonads. Two types of these granules are recognized: (1) granules with homogeneous content and (2) granules containing loose shapeless material in the form of thick cords. The transformation of type two granules into larger structures, as well as the consolidation of type one and type two granules at later stages of oocyte development, are probably the processes that lead to the formation of the characteristic structure and contact plate, visible in paraffin and semithin sections. It remains unclear where exactly the contact plate is localized at the moment of fertilization: inside or outside the oocyte. The content of granules and components of the plate specifically bind the antibodies (RA47) against mesoglein, the ZP domain-containing protein of the mesoglea of A. aurita. The contact plate, covering only the anomalous pole of the oocyte but detected by the presence of ZP domain-containing proteins, may prove to be the simplest egg membrane of the zona pellucida type.

  17. Quantifying the predation on sardine and hake by cetaceans in the Atlantic waters of the Iberian peninsula

    Science.gov (United States)

    Begoña Santos, M.; Saavedra, Camilo; Pierce, Graham J.

    2014-08-01

    Construction of ecosystem models requires detailed information on trophic interactions which may not be readily available, especially for top predators such as cetaceans. Such information can also be useful to estimate natural mortality (M) for fish stock assessments and to evaluate the potential for competition between cetaceans and fisheries. In the present paper we provide estimates and confidence limits, taking into account sampling error, for consumption of fish by the four most common cetaceans along the Atlantic coast of the Iberian Peninsula, while highlighting the uncertainties and biases inherent in the information presently available on energy requirements, diet and population size. We estimated that common dolphins (Delphinus delphis) consume around 6800 (95% CI, 4871-9476) tons of sardine (Sardina pilchardus), 8800 (6195-12,647) tons of gadids, 1100 (721-1662) tons of hake (Merluccius merluccius) and 1900 (1222-2752) tons of scads (Trachurus sp.) annually. For striped dolphins (Stenella coeruleoalba), prey consumed were 900 (196-2661) tons of sardine, 6200 (3448-11,129) tons of gadids, 200 (11-504) tons of hake and 1600 (0-5318) tons of scads. Estimated amounts taken by harbour porpoises (Phocoena phocoena) and bottlenose dolphins (Tursiops truncatus) are much lower, reflecting their low abundance in the area. Cetacean predation on sardine represents 2-8% of the current M value, indicating that cetaceans probably have little influence on sardine population dynamics. For the southern hake stock, estimated average removal by cetaceans often exceeds M. While this may indicate that both M and the consumption estimates for hake require revision it also suggests that cetaceans could have a more significant impact on hake populations. Different approaches to estimation of energy requirements of cetaceans can result in figures that differ by at least a factor of 2. The lack of good estimates of field metabolic rate for most species probably represents the most

  18. Occurrence of Sarcocystis spp. in opossums (Didelphis aurita and Didelphis albiventris in regions of the State of São Paulo, Brazil

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    Renata Assis Casagrande

    2009-04-01

    Full Text Available O objetivo deste estudo foi de determinar a ocorrência de Sarcocystis spp. em D. albiventris e D. aurita em três regiões do Estado de São Paulo. Para tal, utilizou-se noventa e oito Didelphis mortos, sendo 66 D. aurita e 32 D. albiventris, e também 28 D. aurita e cinco D. albiventris vivos. O método de centrífugo-flutuação em solução de sacarose foi empregado para isolamento dos oocistos/esporocistos de Sarcocystis spp. do intestino delgado e das fezes. Encontrou-se Sarcocystis spp. no intestino delgado de 9,1% dos D. aurita (6/66, sendo que quatro destes também houve positividade nas fezes. Não houve diferença estatística significativa entre machos e fêmeas positivos (P= 0,522, e entre os positivos de diferentes origens do Estado de São Paulo (P= 0,627, quanto a ocorrência de Sarcocystis spp. Entretanto, houve diferença estatística significativa entre animais de vida livre e de cativeiro (P = 0.009, sendo que somente os de vida livre foram positivos. Entre adultos e filhotes positivos também houve diferença (P= 0,004, sendo os adultos mais parasitados que os filhotes. Das amostras provenientes dos 28 D. aurita vivos, encontrou-se Sarcocystis spp. em 7.1% (2/28 deles. Dos 32 D. albiventris, todos foram negativos para Sarcocystis spp. nas amostras de intestino delgado e fezes. Os cincos D. albiventris vivos também foram negativos. Sendo assim, pode-se observar que a ocorrência de Sarcocystis spp. em D. aurita e D. albiventris nestas três regiões do Estado de São Paulo é baixa para estas condições analisadas.

  19. Aurelin, a novel antimicrobial peptide from jellyfish Aurelia aurita with structural features of defensins and channel-blocking toxins.

    Science.gov (United States)

    Ovchinnikova, Tatiana V; Balandin, Sergey V; Aleshina, Galina M; Tagaev, Andrey A; Leonova, Yulia F; Krasnodembsky, Eugeny D; Men'shenin, Alexander V; Kokryakov, Vladimir N

    2006-09-22

    A novel 40-residue antimicrobial peptide, aurelin, exhibiting activity against Gram-positive and Gram-negative bacteria, was purified from the mesoglea of a scyphoid jellyfish Aurelia aurita by preparative gel electrophoresis and RP-HPLC. Molecular mass (4296.95 Da) and complete amino acid sequence of aurelin (AACSDRAHGHICESFKSFCKDSGRNGVKLRANCKKTCGLC) were determined. Aurelin has six cysteines forming three disulfide bonds. The total RNA was isolated from the jellyfish mesoglea, RT-PCR and cloning were performed, and cDNA was sequenced. A 84-residue preproaurelin contains a putative signal peptide (22 amino acids) and a propiece of the same size (22 amino acids). Aurelin has no structural homology with any previously identified antimicrobial peptides but reveals partial similarity both with defensins and K+ channel-blocking toxins of sea anemones and belongs to ShKT domain family.

  20. Metabolism measurements of Aurelia aurita planulae larvae, and calculation of maximal survival period of the free swimming stage

    Science.gov (United States)

    Schneider, G.; Weisse, T.

    1985-03-01

    Respiration, ammonia and phosphate excretion experiments were performed with planula larvae of Aurelia aurita (Scyphozoa) from Kiel Fjord, Baltic Sea, in summer 1983. The mean respiration measured was 3.22 nl O2 ind-1 h-1 (at ˜ 20 °C). Excretion experiments revealed average values of 11.41 pM NH4-N ind-1, and 0.92 pM PO4-P ind-1h-1, respectively. The atomic C:N:P ratio of excretion products was 133:10:1. The O:N ratio of 25:1 and O:P ratio of 313:1 point to a lipid-carbohydrate-oriented catabolism of the Aurelia larvae. On the basis of experimental results and of biomass determinations, the maximal survival period of the non-feeding free swimming planula stage was calculated. Typically, the value lies in the range of some days to one week.

  1. Induction of stolon settlement in the scyphopolyps of Aurelia aurita (Cnidaria, Scyphozoa, Semaeostomeae) by glycolipids of marine bacteria

    Science.gov (United States)

    Schmahl, G.

    1985-06-01

    The settlement of pedal stolons of scyphopolyps of Aurelia aurita Lamarck could be induced by addition of a species of bacteria from the family Micrococcaceae. After treatment of the bacteria with several organic solvents a crude lipid extract free of bacteria could be obtained which was shown to be effective in inducing stolon settlement. Crude lipids extracted from the late logarithmic growth phase had an optimal effect on stolon attachment, in contrast to previously published experiments showing that all logarithmic phases of bacteria had the same level of effectiveness. After separation of the crude lipid extracts by thin layer chromatography and subsequent bioassay of the reeluated substances, acylgalactosidyldiglyceride and monogalactosidyldiglyceride were identified as the effective substances. Monogalactosidyldiglyceride was only found in bacteria from the medium logarithmic growth phase, whereas the former was found at all stages. The effectiveness of acylgalactosidyldiglyceride was independent of the growth phase of the extracted bacteria.

  2. KANDUNGAN ASAM AMINO, TAURIN, MINERAL MAKRO-MIKRO, DAN VITAMIN B12 UBUR-UBUR (Aurelia aurita SEGAR DAN KERING

    Directory of Open Access Journals (Sweden)

    - - Nurjanah

    2014-06-01

    Full Text Available Tujuan penelitian adalah menentukan komposisi gizi, asam amino, taurin, mineral makro dan mikro,dan vitamin B12 pada ubur-ubur (Aurelia aurita segar dan kering. Asam amino esensial pada ubur-uburyaitu arginina, leusina, valina, treonina, lisina, isoleusina, fenilalanina, metionina, dan histidina, sedangkanasam amino non esensial yaitu asam glutamat, glisina, asam aspartat, serina, alanina, dan tirosina. Asamamino esensial tertinggi segar dan kering adalah arginina sebesar 1,72% (bk dan 1,44% (bk dan terendahhistidina yaitu sebesar 0,19% (bk dan 0,13% (bk. Asam amino non esensial segar dan kering tertinggiadalah asam glutamat dan glisina yaitu sebesar 3,26% (bk dan 2,62% (bk dan terkecil tirosina sebesar0,38% (bk dan 0,41% (bk. Taurin segar sebesar 2,68% (bk dan kering sebesar 0,67% (bk. Mineral makrotertinggi segar dan kering adalah natrium yaitu 180.092,1 ppm (bk dan 111.209,4 ppm (bk, terkecil adalahkalsium yaitu 5.750,2 ppm (bk dan 11,1 ppm (bk. Mineral mikro tertinggi segar dan kering adalah iodium yaitu8.291,5 ppm (bk dan 1.800 ppm (bk dan yang terkecil adalah tembaga yaitu 1,1 ppm (bk dan 0,6 ppm (bk.Vitamin B12 segar adalah 396,6 μm/100 g (bk dan kering 63,5 μm/100 g (bk.Kata kunci: asam amino, mineral, taurin, ubur-ubur (Aurelia aurita, vitamin B12

  3. An experimental study of Aurelia aurita feeding behaviour: Inference of the potential predation impact on a temperate estuarine nursery area

    Science.gov (United States)

    Pereira, Rita; Teodósio, Maria Alexandra; Garrido, Susana

    2014-06-01

    Temperate estuaries are nursery areas for economically important fisheries resources. The common jellyfish Aurelia aurita is a resident species in many of these areas, where it can reach high abundances. This work aimed to determine the potential for predation of A. aurita on zooplanktonic organisms and early life stages of fishes, measuring feeding rates at concentrations that mimic those occurring for zooplankton, fish eggs and larvae in an estuarine nursery area. A set of experiments was aimed at determining the feeding selectivity of jellyfish when offered a mixture of fish eggs and larvae and wild plankton. Clearance rates varied markedly with prey availability and concentrations. When given mixtures of different prey types, jellyfish preferentially elected some taxa (copepods and fish eggs). Data obtained in the laboratory experiments were used to infer the potential impact of jellyfish predation upon zooplankton and ichthyoplankton in the Guadiana estuary (Southern Iberia). Repeated sampling of zooplankton, fish eggs and medusae was undertaken during the summer season of 2011. Abundance determinations were combined with experimentally estimated clearance rates of individual medusa to infer the potential jellyfish-induced mortality on prey in the area. In June and early August jellyfish-induced mortality rates were very high, and half-life times (t1/2) were consequently short for the zooplankton and ichthyoplankton. Although the potentially overestimation of our feeding rates typical of confined laboratory experiments, the results show high ingestion and clearance rates at high temperatures, typical from summer condition, and results also suggest that either by predation on early life stages of fish, or by competition for food resources, jellyfish may have a significant impact on estuarine communities and its nursery function.

  4. Demonstration of a fully-coupled end-to-end model for small pelagic fish using sardine and anchovy in the California Current

    Science.gov (United States)

    Rose, Kenneth A.; Fiechter, Jerome; Curchitser, Enrique N.; Hedstrom, Kate; Bernal, Miguel; Creekmore, Sean; Haynie, Alan; Ito, Shin-ichi; Lluch-Cota, Salvador; Megrey, Bernard A.; Edwards, Chris A.; Checkley, Dave; Koslow, Tony; McClatchie, Sam; Werner, Francisco; MacCall, Alec; Agostini, Vera

    2015-11-01

    We describe and document an end-to-end model of anchovy and sardine population dynamics in the California Current as a proof of principle that such coupled models can be developed and implemented. The end-to-end model is 3-dimensional, time-varying, and multispecies, and consists of four coupled submodels: hydrodynamics, Eulerian nutrient-phytoplankton-zooplankton (NPZ), an individual-based full life cycle anchovy and sardine submodel, and an agent-based fishing fleet submodel. A predator roughly mimicking albacore was included as individuals that consumed anchovy and sardine. All submodels were coded within the ROMS open-source community model, and used the same resolution spatial grid and were all solved simultaneously to allow for possible feedbacks among the submodels. We used a super-individual approach and solved the coupled models on a distributed memory parallel computer, both of which created challenging but resolvable bookkeeping challenges. The anchovy and sardine growth, mortality, reproduction, and movement, and the fishing fleet submodel, were each calibrated using simplified grids before being inserted into the full end-to-end model. An historical simulation of 1959-2008 was performed, and the latter 45 years analyzed. Sea surface height (SSH) and sea surface temperature (SST) for the historical simulation showed strong horizontal gradients and multi-year scale temporal oscillations related to various climate indices (PDO, NPGO), and both showed responses to ENSO variability. Simulated total phytoplankton was lower during strong El Nino events and higher for the strong 1999 La Nina event. The three zooplankton groups generally corresponded to the spatial and temporal variation in simulated total phytoplankton. Simulated biomasses of anchovy and sardine were within the historical range of observed biomasses but predicted biomasses showed much less inter-annual variation. Anomalies of annual biomasses of anchovy and sardine showed a switch in the mid

  5. Trophic structure of pelagic species in the northwestern Mediterranean Sea

    Science.gov (United States)

    Albo-Puigserver, Marta; Navarro, Joan; Coll, Marta; Layman, Craig A.; Palomera, Isabel

    2016-11-01

    Ecological knowledge of food web interactions within pelagic marine communities is often limited, impairing our capabilities to manage these ecologically and economically important marine fish species. Here we used stable isotope analyses to investigate trophic interactions in the pelagic ecosystem of the northwestern Mediterranean Sea during 2012 and 2013. Our results suggest that European sardine, Sardina pilchardus, and anchovy, Engraulis encrasicolus, are consumers located at relatively low levels of the pelagic food web. Unexpectedly, the round sardinella, Sardinella aurita, appeared to be located at a higher trophic level than the other small pelagic fish species, although previous studies found similarity in their diets. Isotope data suggested that trophic niches of species within the genera Trachurus spp. and Scomber spp., were distinct. Atlantic bonito Sarda sarda, European hake Merluccius merluccius and European squid Loligo vulgaris, appeared to feed at higher trophic levels than other species. Despite some intraspecific seasonal variability for some species, community trophic structure appeared relatively stable through the year. These data provide an important step for developing models of food web dynamics in the northwestern Mediterranean Sea.

  6. [Fatty acid composition and cholesterol content in naturally canned jurel, sardine, salmon, and tuna].

    Science.gov (United States)

    Romero, N; Robert, P; Masson, L; Luck, C; Buschmann, L

    1996-03-01

    To obtain more information about fatty acid profile and cholesterol content of fat extracted from canned fish in brine habitually consumed in Chile, four different species Jurel (Trachurus murphyi), Sardine (Sardinops sagax), Salmon (Oncorhynchus kisutch) and Tuna (Thunnus alalunga) were analyzed. The GLC of fatty acid methyl esters showed that the main group of fatty acids belongs to polyunsaturated, being omega-3 family the more important. The principal representants were eicosapentaenoic (EPA) and docosahexaenoic acids (DHA), with percentages between 5%-11% and 12%-22% respectively. Omega-6 family was represented mainly by arachidonic acid (AA) with percentages between 2%-4%. Cholesterol content was similar to the values found in other animal origen meats. The figures were between 41-86 mg of cholesterol per 100 g of edible product, Tuna in brine, was the product with the lowest content of cholesterol. The calculated amount of EPA, DHA and total omega-3 fatty acids indicated values between 95-604, 390-1163 and 609-2775 mg respectively per 100 g of edible product. Due these results is important to emphasize the consumption of this type of canned fish in brine, that they really represent a good dietary source of mainly polyunsaturated omega-3 fatty acids. The international recommendations indicate to increase the consumption of fish, due the beneficial effects described in relation with cardiovascular disease, which is the mean cause of death in Chile, country with a wide variety of marine origen foods, but with a contradictory answer about its consumption which is not incorporated in the current diet.

  7. Pascale Sardin. Samuel Beckett et la passion maternelle ou l’hystérie à l’œuvre

    Directory of Open Access Journals (Sweden)

    Jean-Christophe MURAT

    2010-03-01

    Full Text Available La parution d’un ouvrage critique sur Samuel Beckett tombe évidemment à point nommé, puisque certaines des grandes pièces de ce dramaturge figurent au programme de deux concours de recrutement (session 2010 : Endgame pour l’agrégation externe d’Anglais, En attendant Godot et Oh les beaux jours pour l’agrégation externe de Lettres modernes. Il serait pourtant erroné de voir dans le livre de Pascale Sardin une publication « opportuniste » à destination des seuls candidats des concours. La pers...

  8. Dans l’atelier de l’écrivain (autofictif. L’enterrement de la sardine de Patrice Lessard

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    Marilyn Randall

    2017-03-01

    Full Text Available L’enterrement de la sardine, dernier roman de la trilogie lisboète de Patrice Lessard, nous introduit à la fois dans le labyrinthe de la vieille ville de Lisbonne et dans celui de la narration de deux romans en train de se faire. La distinction entre fiction et autobiographie proposée dans l’avant-propos s’effondre vite devant les multiples intrusions du « réel » dans la fiction et de la fiction dans le « réel ». De plus, la facture fragmentaire de l’ensemble pose un défi au lecteur qui se trouve en plein atelier de l’écrivain, devant un portrait de l’artiste inachevé, inachevable. L’enterrement de la sardine, the final volume of Patrick Lessard’s Lisbonne trilogy, plunges the reader into the labyrinth of Lisbonne’s old city as well as into the meanders of two novels in the making. The distinction between fiction and autobiography presented in the preface quickly dissolves in the face of the multiple intrusions of the “real” world into the fictional one and vice versa. The fragmentary nature of the entire text poses a challenge to the reader, who seems to have wandered into the writer’s workshop to confront an unfinished — perhaps unfinishable — self-portrait.

  9. Optimization of α-tocopherol and ascorbyl palmitate addition for the stabilization of sardine oil

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    Morales-Medina, R.

    2015-06-01

    Full Text Available The purpose of the present work was to optimize the addition of natural antioxidants (α- tocopherol and ascorbyl palmitate for the stabilization of sardine oil rich in omega-3 PUFA. The optimal values for peroxide value (PV, which minimizes primary oxidation products, were obtained at low concentrations of α-tocopherol (50–207 ppm, high content of ascorbyl palmitate (450 ppm and 50 ppm citric acid. On the other hand, optimal values for p-anisidine value (AV, which minimizes secondary oxidation products, were found at medium concentrations of α-tocopherol (478–493 ppm, high contents of ascorbyl palmitate (390–450 ppm and 50 ppm citric acid. The conflicting effect of α-tocopherol on the individual optimization of PV and AV motivated the generation of a Pareto front (set of non inferior solutions employing the weighted-sum multi-objective optimization technique.El objetivo de este trabajo fue optimizar la adición de antioxidantes naturales (α-tocoferol y palmitato de ascorbilo para la estabilización de aceite de sardina rico en omega-3 PUFA. Bajas concentraciones de α-tocoferol (50–207 ppm combinadas con la adicción de antioxidantes secundarios como palmitato de ascorbilo (450 ppm y ácido cítrico (50 ppm, minimizaron la formación de hidroperóxidos en el aceite de sardina estudiado. Sin embargo, los productos secundarios de oxidación se redujeron para concentraciones medias de α-tocoferol (478–493 ppm, altas de palmitato de ascorbilo (390–450 ppm y 50 ppm de ácido cítrico. El efecto contradictorio de la concentración de α-tocoferol en la optimización individual del índice de peróxidos e índice de p-anisidina motivó la realización de una optimización simultánea que permite satisfacer la optimización de cada una de las variables individuales en el grado deseado.

  10. Sardine oil loaded vanillic acid grafted chitosan microparticles, a new functional food ingredient: attenuates myocardial oxidative stress and apoptosis in cardiomyoblast cell lines (H9c2).

    Science.gov (United States)

    Vishnu, K V; Ajeesh Kumar, K K; Chatterjee, Niladri S; Lekshmi, R G K; Sreerekha, P R; Mathew, Suseela; Ravishankar, C N

    2017-08-02

    Fish oil has been widely recognized as an excellent dietary source of polyunsaturated n-3 fatty acids such as EPA and DHA. However, it can undergo oxidation easily resulting in the formation of toxic off flavor compounds such as hydroperoxides. These compounds adversely affect the nutritional quality and may induce several stress reactions in body. To solve this problem, a new antioxidant bio-material, vanillic acid-grafted chitosan (Va-g-Ch), was synthesized and used as a wall material for microencapsulation of fish oil. The sardine oil loaded Va-g-Ch microparticles could be a potential functional food ingredient considering the numerous health benefits of fish oil, chitosan, and vanillic acid. The current study aimed to investigate the possible protective effect of sardine oil-loaded Va-g-Ch microparticles against doxorubicin-induced cardiotoxicity and the underlying mechanisms. In vitro cytotoxicity evaluation was conducted using H9c2 cardiomyocytes. MTT assay revealed that effective cytoprotective effect was induced by a sample concentration of 12.5 μg/mL. Results of apoptosis by double fluorescent staining with acridine orange/ethidium bromide and caspase-3 evaluation by ELISA substantiated the above findings. Further, flow cytometric determination of membrane potential, relative expression of NF-κB by PCR, and ROS determination using DCFH-DA also confirmed the protective effect of encapsulated sardine oil against doxorubicin-induced cardiotoxicity. NF-κB expression was down-regulated nearly by 50% on cells treated with encapsulated sardine oil. Altogether, the results revealed that sardine oil-loaded Va-g-Ch microparticles demonstrated potential cell protection against doxorubicin-induced oxidative stress.

  11. Endocrine cells and nerve ganglia of the small intestine of the Opossum Didelphis aurita Wied-Neuwied, 1826 (Mammalia: Didelphidae

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    Gláucia M. Freitas-Ribeiro

    2012-09-01

    Full Text Available The nervous and endocrine systems jointly control intestinal movements, secretions of their glands and also participate of the processes of nutrient digestion and absorption. Therefore, the central objective of this study was to verify the existence of a possible relationship between the number of nervous cells and ganglia of the submucosal and myenteric plexuses and the number of endocrine cells in the small intestine of adult D. aurita. The utilized staining techniques were Grimelius, modified Masson-Fontana, direct immunoperoxidase and H-E. Argyrophillic, argentaffin and insulin immunoreactive endocrine cells do not numerically vary between the initial, mid and final regions of the duodenum, jejunum and ileum (P>0.05, except for argyrophillic cells in the jejunum (P>0.05. No numerical relationship has yet been verified between the number of nerve ganglia and endocrine cells, and also between nervous and endocrine cells. We recommended the use of new immunohistochemical techniques to confirm the numerical correlation between the nervous and endocrine systems in the small intestine. The morphology and distribution of endocrine cells and the nerve ganglia studied were similar to those encountered in eutherian mammals.Os sistemas nervoso e endócrino controlam integra-damente os movimentos intestinais, a secreção de suas glândulas e também participam dos processos de digestão e absorção de nutrientes. Portanto, o objetivo central deste estudo foi verificar a existência de uma possível relação entre o número de células nervosas e gânglios dos plexos submucosos e mioentéricos e o número de células endócrinas no intestino delgado de adultos de D. aurita. As técnicas de coloração utilizadas foram Grimelius, Masson-Fontana modificada, imunoperoxidase direta e H-E. As células endócrinas argirófilas, argentafins e imunorreativas à insulina não variaram numericamente entre as regiões inicial, média e final do duodeno, jejuno e

  12. Distribution of moon jellyfish Aurelia aurita in relation to summer hypoxia in Hiroshima Bay, Seto Inland Sea

    Science.gov (United States)

    Shoji, Jun; Kudoh, Takaya; Takatsuji, Hideyuki; Kawaguchi, Osamu; Kasai, Akihide

    2010-02-01

    Biological and physical surveys were conducted in order to investigate the relationship between environmental conditions and the distribution of moon jellyfish Aurelia aurita in Hiroshima Bay, western Seto Inland Sea, Japan. Moon jellyfish and ichthyoplankton were collected at 13 stations in Hiroshima Bay during monthly surveys from July to September in 2006 and 2007. Surface temperature in 2006 was significantly lower during the August and September cruises and surface salinity was lower during all cruises than in 2007. Moon jellyfish was the most dominant gelatinous plankton collected, accounting for 89.7% in wet weight. Mean moon jellyfish abundance in 2006 was higher than that in 2007 from July through September, with significant inter-year differences for July and September. Variability in precipitation and nutritional input from the Ohta River, northernmost part of Hiroshima Bay, were suggested as possible factors affecting the inter-annual variability in moon jellyfish abundance in the coastal areas of northern Hiroshima Bay. Moon jellyfish were more abundant in the coastal areas of northern Hiroshima Bay, where the dissolved oxygen (DO) concentration was lower, while low in the central part of the bay. Japanese anchovy Engraulis japonicus eggs were most dominant (58.1% in number) among the ichthyoplankton and were abundant in the central area of Hiroshima Bay. Explanatory analysis was conducted to detect possible effects of environmental conditions on the abundance of moon jellyfish and Japanese anchovy eggs during the summer months in Hiroshima Bay. Of the environmental conditions tested (temperature, salinity and DO of surface and bottom layers at each sampling station), bottom DO had the most significant effect on the moon jellyfish abundance: there was a negative correlation between the bottom DO and the moon jellyfish abundance in Hiroshima Bay during summer.

  13. Estimation of the minimum food requirement using the respiration rate of medusa of Aurelia aurita in Sihwa Lake

    Science.gov (United States)

    Han, Chang-hoon; Chae, Jinho; Jin, Jonghyeok; Yoon, Wonduk

    2012-06-01

    We examined the respiration rate of Aurelia aurita medusae at 20 °C and 28 °C to evaluate minimum metabolic demands of medusae population in Sihwa Lake, Korea during summer. While weight specific respiration rates of medusae were constant and irrespective to the wet weight (8-220 g), they significantly varied in respect to temperatures ( p<0.001, 0.11±0.03 mg C g-1 of medusa d-1 at 20°C and 0.28±0.11 mg C g-1 of medusa d-1 at 28 °C in average, where Q 10 value was 2.62). The respiration rate of medusae was defined as a function of temperature ( T, °C) and body weight ( W, g) according to the equation, R=0.13×2.62( T-20)/10 W 0.93. Population minimum food requirement ( PMFR) was estimated from the respiration rate as 15.06 and 4.86 mg C m-3 d-1 in June and July, respectively. During this period, increase in bell diameter and wet weight was not significant ( p=1 in the both), suggesting that the estimated PMFR closely represented the actual food consumption in the field. From July to August, medusae grew significantly at 0.052 d-1, thus the amount of food ingested by medusae population in situ was likely to exceed the PMFR (1.27 mg C m-3 d-1) during the period. In conclusion, the medusae population of higher density during June and July had limited amount of food, while those of lower in July and August ingested enough food for growth.

  14. Occurrence, inter-annual variability and zooplankton-predation impact of the invasive ctenophore Mnemiopsis leidyi and the native jellyfish Aurelia aurita in Limfjorden (Denmark) in 2010 and 2011

    DEFF Research Database (Denmark)

    Riisgaard, Hans Ulrik; Jaspers, Cornelia; Serre, Sandrine

    2012-01-01

    In 2007, the invasive ctenophore Mnemiopsis leidyi was observed for the first time in Limfjorden (Denmark) where it exhibited mass occurrence in late summer while the indigenous and usually dominating common jellyfish, Aurelia aurita, was nearly absent. Both species were further studied in 2008...

  15. Mitochondrial genome of the moon jelly Aurelia aurita (Cnidaria, Scyphozoa): A linear DNA molecule encoding a putative DNA-dependent DNA polymerase.

    Science.gov (United States)

    Shao, Zhiyong; Graf, Shannon; Chaga, Oleg Y; Lavrov, Dennis V

    2006-10-15

    The 16,937-nuceotide sequence of the linear mitochondrial DNA (mt-DNA) molecule of the moon jelly Aurelia aurita (Cnidaria, Scyphozoa) - the first mtDNA sequence from the class Scypozoa and the first sequence of a linear mtDNA from Metazoa - has been determined. This sequence contains genes for 13 energy pathway proteins, small and large subunit rRNAs, and methionine and tryptophan tRNAs. In addition, two open reading frames of 324 and 969 base pairs in length have been found. The deduced amino-acid sequence of one of them, ORF969, displays extensive sequence similarity with the polymerase [but not the exonuclease] domain of family B DNA polymerases, and this ORF has been tentatively identified as dnab. This is the first report of dnab in animal mtDNA. The genes in A. aurita mtDNA are arranged in two clusters with opposite transcriptional polarities; transcription proceeding toward the ends of the molecule. The determined sequences at the ends of the molecule are nearly identical but inverted and lack any obvious potential secondary structures or telomere-like repeat elements. The acquisition of mitochondrial genomic data for the second class of Cnidaria allows us to reconstruct characteristic features of mitochondrial evolution in this animal phylum.

  16. Use of the space by the opossum Didelphis aurita Wied-Newied (Mammalia, Marsupialia in a mixed forest fragment of southern Brazil

    Directory of Open Access Journals (Sweden)

    Cáceres Nilton Carlos

    2003-01-01

    Full Text Available Use of the space by the opossum Didelphis aurita Wied-Newied, 1826 (Mammalia, Marsupialia in a mixed forest fragment of southern Brazil. The space use of the marsupial Didelphis aurita was studied in a forest fragment of southern Brazil from February 1995 to January 1996. The method used was the 'distribution utilization' in which each trap was set in 38 x 38 m quadrats. Captures of each marked individual in each point give information on its habitat use. Food availability was searched and compared to the habitat utilization and to the food consumption of opossums. Distribution patterns of captures (aggregated to random and spatial overlap between individuals were searched. Results showed aggregated distributions of individuals, particularly females, in the fragment. Females used exclusively the fragment during the drier season. Opossums tend to not choose the sites with highest food availability to establish home ranges. Spatial overlap was usually low between forest resident and neighbouring resident females, but much lower during the breeding season (only forest resident females in an apparently pattern of territoriality. Hence, core areas of females decreased in size during the breeding season. Males probably searched primarily for mates during the breeding season being less opportunistic than females in feeding habits, yet their space use did not correlate to food consumption.

  17. Constant harvest rate for the Pacific sardine (Sardinops caeruleus) fishery in the Gulf of California based on catchability-at-length estimations

    OpenAIRE

    2009-01-01

    Constant harvest rate as a management strategy for the Pacific sardine (Sardinops caeruleus) fishery in the Gulf of California is supported by an analysis of variations in the catchability coefficient (q), stock abundance and commercial catch. Catchability was analyzed based on population length-structured data standard length (SL), expressed as CPUE, for 26 fishing seasons (1972-1973 to 1997-1998). We used a deterministic model of catchability to estimate the catchability-at-length assuming ...

  18. SÍNTESE E CARACTERIZAÇÃO DE BIODIESEL COM MATERIAL LIPÍDICO EXTRAIDO DAS VÍSCERAS DA Sardinella Brasiliensis

    Directory of Open Access Journals (Sweden)

    Anderson Fernandes Gomes

    2015-06-01

    Full Text Available Este trabalho relata a extração atraves dois métodos (a frio e a quente, com solventes orgânicos o material lipídico obtido das vísceras da Sardinella Brasiliensis. Os lipidios foram submetidos a uma hidroesterificação utilizando iodo sublimado como catalisador. O óleo e o biodiesel foram caracterizados por RMN H1, espectroscopia FTIR, tensão superficial, densidade e água e sedimentos. Os resultados de água e sedimentos e densidade foram comparados com os previstos na legislação. Os resultados evidenciaram que o método à frio forneceu melhor rendimento na extração do óleo, que ocorreu a hidroesterificação do material indicado na técnica de  RMN H1 pelos dois prótons com sinal de quarteto na região de 4,2 ppm referentes aos hidrogênios metilênicos (CH2 ɑ-O, característicos de mono ésteres etílicos (biodiesel e na técnica de FTIR pelo aparecimento de deformações axiais características de ésteres em 1.742 cm-1.  Assim concluiu-se que as vísceras da sardinha são uma matéria-prima promissora e barata para produção de biodiesel, no entanto há necessidade de melhor caracterização da sua qualidade físico-química e estabilidade.

  19. Perfil hematológico de gambás Didelphis aurita e D. albiventris do Estado de São Paulo, Brasil = Blood profile of opossums Didelphis aurita and D. albiventris of São Paulo State, Brazil

    Directory of Open Access Journals (Sweden)

    Renata Assis Casagrande

    2009-04-01

    Full Text Available O objetivo deste trabalho foi fornecer dados sobre o perfil hematológico de gambás (Didelphis sp.. Realizou-se hemograma de 83 D. aurita e 35 D. albiventris. Os valores obtidos dos D. aurita foram: Hematócrito dos adultos(A 31,8 ± 8,0% e dos filhotes(F 30,2 ± 6,9%; Eritrócitos A: 4,3 ± 1,5 x 106 μL-1, F: 3,4 ± 1,1 x 106 μL-1; Proteínas Totais A: 8,5 ± 1,0 g dL-1, F: 7,2 ± 0,9 g dL-1;Hemoglobina A: 10,9 ± 3,4 g dL-1, F: 8,7 ± 2,2 g dL-1; VCM A: 78,13 ± 18,13, F: 93,65 ± 23,62; HCM A: 26,41 ± 6,42, F: 27,09 ± 7,44; CHCM A: 64,59 ± 8,12, F: 30,40 ± 11,49; Leucócitos A: 8.205 ± 4.950 μL-1, F: 5.126 ± 3.945 μL-1; Neutrófilos A: 2.761 ± 2.966 μL-1, F: 1.310± 2.283 μL-1; Linfócitos A: 3.653 ± 2.431 μ L-1, F: 3.239 ± 2.234 μL-1; Monócitos A: 363,2 ± 308,7 μL-1, F: 101,5 ± 107,9 μL-1; Eosinófilos A: 1.362,0 ± 1.114,0 μL-1, F: 456,1 ± 464,4 μL-1 e Basófilos A: 65,9 ± 127,0 μL-1, F: 19,8 ± 48,7 μL-1. Os valores obtidos dos D. albiventris foram: Hematócrito A: 33,2 ± 14,0%, F: 25,7 ± 3,7%; Eritrócitos A: 4,8 ± 1,7 x 106 μL-1 , F: 3,9 ± 1,5 x 106 μL-1; Proteínas Totais A: 8,2 ± 0,6 g dL-1, F: 7,9 ± 1,1 g dL-1; Hemoglobina A: 10,3 ± 2,8 g dL-1, F: 10,2 ± 2,7 g dL-1; VCM A: 71,34 ± 23,74, F: 74,88 ± 27,68; HCM A: 22,86 ± 9,09, F: 28,63 ± 9,27,CHCM A: 32,48 ± 2,43, F: 40,13 ± 0,42; Leucócitos A: 11.683 ± 7.245 μL-1, F: 4.667 ± 4.027 μL-1; Neutrófilos A: 6.007 ± 6.250 μL-1, F: 1.274 ± 2.586 μL-1; Linfócitos A: 4.389 ± 1.928 μL-1, F: 3.151± 3.045 μL-1; Monócitos A: 455 ± 378 μL-1, F: 152,7 ± 180,4 μL-1; Eosinófilos A: 803 ± 840 μL-1, F: 67,8 ± 91,4 μL-1 e Basófilos A: 30,8 ± 75,3 μL-1, F: 21,5 ± 56,6 μL-1.The objective of this study was to provide data on the blood profile of opossums (Didelphis sp.. Hemograms were performed of 83 D. aurita and 35 D. albiventris. The results of D. aurita were: Hematocrit for adults(A 31.8 ± 8.0%, and for offspring(F 30.2 ± 6

  20. KUALITAS MUTU BAHAN MENTAH DAN PRODUK AKHIR PADA UNIT PENGALENGAN IKAN SARDINE DI PT. KARYA MANUNGGAL PRIMA SUKSES MUNCAR BANYUWANGI

    Directory of Open Access Journals (Sweden)

    Dyah Agustin Wulandari

    2009-04-01

    Full Text Available Mutu ikan kaleng tergantung pada kesegaran bahan mentah, cara pengalengan, peralatan dan kecakapan serta pengetahuan pelaksana-pelaksana teknis, sanitasi dan higiene pabrik dan lingkungan.  Kesegaran bahan mentah sangat penting dalam industri perikanan.  Kesegaran adalah tolak ukur untuk membedakan ikan jelek dan bagus kualitasnya.  Bila kualitas bahan mentah bagus, maka produk yang dihasilkan juga bagus.  Untuk mengendalikan mutu produk yang dihasilkan perusahaan diperlukan suatu sistem yang terkendali dan dapat mengendalikan seluruh aktifitas yang mempengaruhi mutu produk. Khusus untuk produk perikanan lahirnya konsep HACCP mendorong negara-negara maju menerapkan sistem pengawasan mutu ini kepada produsen sebagai jaminan mutu produknya. Dari hasi studi pustaka dan penelitian serta pengamatan langsung pada PT. Karya Manunggal Prima Sukses Muncar,  ternyata diketahui bahwa mutu bahan baku dan produk akhir berupa ikan kaleng sardine saus tomat yang dihasilkan sesuai dengan standart mutu SNI 01-3548-1994.  Sedangkan penerapan konsepsi HACCP belum terlaksana dengan baik sehingga diperlukan perbaikan, baik GMP dan SSOP pada unit pengolahan.Kata kunci : Mutu ikan kaleng, HACCP.  The quality of a certain tinned-fish depends on several things; those are the freshness of raw materials, tinning technique, devices, knowledge, and capability of the technicians, sanitation and hygienist of the factory. The freshness of raw materials is important in fishery industries.  Freshness is one of indicators in determining fish quality. If raw materials are in good quality so that the product will be. To maintain quality of a product in certain factory, system that are able to control all of the elements affecting product quality, is absolutely needed. In fishery product, availability of HACCP, encourage the advanced-countries to apply a monitoring system of product quality to the producer as a kind of quality guarantee. From the study of certain literature

  1. Glycerolysis of sardine oil catalyzed by a water dependent lipase in different tert-alcohols as reaction medium

    Directory of Open Access Journals (Sweden)

    Solaesa, Á. G.

    2015-12-01

    Full Text Available The production of monoacylglycerol rich in polyunsaturated fatty acids (PUFA via enzymatic glycerolysis of sardine oil in a homogeneous system was evaluated. Reactions were conducted in two different tert-alcohols. Based on the phase equilibrium data, the amount of solvent added to create a homogeneous system has been calculated and optimized. The immobilized lipase used in this work was Lipozyme RM IM from Rhizomucor miehei, a water dependent lipase. The amount of water added as well as other reaction parameters were studied to evaluate the optimum conditions for monoacylglycerol obtencion. An initial reactant mole ratio glycerol to sardine oil 3:1, 12 wt% of water based on glycerol content and 10 wt% of lipase loading (based on weight of reactants, achieved a MAG yield of around 70%, with nearly 28 wt% PUFA, with low free fatty acid content (lower than 18 wt%.En este trabajo se ha estudiado la producción de monoacilglicéridos, ricos en ácidos grasos poliinsaturados (AGPI, mediante glicerolisis enzimática de aceite de sardina. La reacción se ha llevado a cabo en dos tert-alcoholes para conseguir de esta forma un medio homogéneo de reacción. La cantidad de disolvente añadida al medio de reacción se ha optimizado y calculado en base al equilibrio de fases de los componentes del sistema. La lipasa empleada como biocatalizador ha sido la enzima inmovilizada Lipozyme RM IM de Rhizomucor miehei, una lipasa dependiente de agua. Se ha estudiado el efecto de distintos parámetros cinéticos, así como de la cantidad de agua añadida al medio de reacción, en la producción de monoacilglicéridos. De los resultados obtenidos, se puede concluir que, para una relación molar inicial de reactantes glicerol:aceite de sardina de 3:1, un 12 % en peso de agua en base al glicerol y un 10 % en peso de lipasa, en base al peso de reactantes; se puede llegar a conseguir un rendimiento en monoacilglicéridos alrededor del 70 % en peso, con casi un 28 % en

  2. A preliminary analysis of the state of exploitation of the sardine, Sardina pilchardus (Walbaum, 1792, in the gulf of Annaba, East Algerian

    Directory of Open Access Journals (Sweden)

    Bedairia, A.

    2009-12-01

    Full Text Available This study was performed on 2,859 specimens of sardine, Sardina pilchardus, collected biweekly from November 2006 to October 2007. Samplings were carried out at the fishing port of Annaba where purse-seine methods are used for small-scale fishing at depths from 15 to 30 m. Data concerning the exploitation of catches were analysed by means of two software packages: i FISAT (2004, which we used to determine the essential parameters for the study of dynamics; and ii VIT (2000, the most suitable tool for stock assessment based on the application of length cohort analysis (LCA together with a yield per recruit analyses (Y/R based on a short series of data. VIT (2000 assumes steady state and functions with pseudo-cohorts, requiring knowledge of the catches over one year only instead of a historical series of several years. The results of this application revealed that the exploitable average biomass of the sardine stock, composed of 28 length sizes from 6.5 to 20 cm with a step of 0.5 cm, was around 4,778.93 tons, of which 2,513 tons (53% were spawning stock. The size and the average age of the sardine stock were 12.5 cm and 2.7 years. Total biomass balance (D was estimated at 5,508.64 tons. This corresponded to growth in weight of 4,453.77 tons, (80.85%, as compared to recruitment of only 1,054.86 tons (19.15%. Losses were caused mainly by natural mortality (M, estimated at 3,823.14 tons, and accounting for 69.40%. This was higher than fishing mortality (F which was 1,685.5 tons (30.60%. We estimated the yield per recruit (Y/R of sardine at 2.682 g. This value was lower than the threshold of maximum yield per recruit at 3.413 g. Though preliminary, these results indicate that the sardine population can be considered to be in a situation of under-exploitation in this area. The stock is moderately exploited for F0.1 a reference considered more appropriate for management. Applying the precautionary principle, fishing efforts should not increase and we

  3. PENGARUH KATALIS BASA (NaOH PADA TAHAP REAKSI TRANSESTERIFIKASI TERHADAP KUALITAS BIOFUEL DARI MINYAK TEPUNG IKAN SARDIN

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    Diah Probo Ningtyas

    2013-06-01

    Full Text Available Biofuel is an alternative diesel engine fuel is produced from oils/fats of plants and animals (including the fisheries industry waste through the esterification and transesterifiksi reactions. A transesterification is reaction to form esters and glycerol from trigliserin (fat/oil and bioalcohol (methanol or ethanol. Transesterification is an equilibrium reaction so that the presence of a catalyst can accelerate the achievement of a state of equilibrium. Process of the transesterification reaction of sardine flour oil waste with NaOH as base catalyst in producing biofuels was conducted.The research purpose has studied the influence of NaOH concentration in transesterification process and examinate its effect on the quality of biofuels production, conversion, and physic quality. The variables that analysed was the effect of NaOH concentration as catalyst (0.5%, 1.0%, 1.5%, and 2.0% from amount of oil and methanol in the transesterification reaction step. The result showed that the increasing NaOH concentration (0.5 - 1.5%, enhanced the biofuel conversion (%. The highest conversion of biofuels was achieved by using 1.50% NaOH (w/w with 45.34% biofuels conversion. The major component in the biofuels was methyl palmitate (20.31%. ASTM analysis data also supported that the biofuel product was in agreement with automotive diesel fuel specification.

  4. Comparison of the feeding apparatus and diet of European sardines Sardina pilchardus of Atlantic and Mediterranean waters: ecological implications.

    Science.gov (United States)

    Costalago, D; Garrido, S; Palomera, I

    2015-04-01

    In this study, the feeding apparatus (gill rakers, GR) and the diet composition of European sardine Sardina pilchardus populations living in two contrasting environments were compared: the upwelling area off western Iberia and the comparatively less productive region of the north-western Mediterranean Sea. The importance of local adaptations in the trophic ecology of this species was estimated. Sardina pilchardus from the Atlantic Iberian coast and from the north-western Mediterranean Sea have clear differences in the feeding apparatus and diet compositions. Those from the Atlantic Iberian coast have significantly more GRs than S. pilchardus of the same size range in the Mediterranean Sea. While S. pilchardus from the Mediterranean Sea mostly depend on prey ranging between 750-1500 and 3000-4000 µm, corresponding mostly to cladocerans, decapods and copepods, those from the Atlantic depend on smaller prey (50-500 and 1000-1500 µm) that include phytoplankton and copepods, particularly during summer months, and S. pilchardus eggs during the winter. The marked difference between the trophic ecology of S. pilchardus in the two areas studied appears to have originated from different dietary strategies that the two populations have adopted in contrasting feeding environments. These differences are shown to profoundly affect the size and quality of prey consumed, and the effect of cannibalism on the populations.

  5. Field comparison of sardine post-flexion larval growth and biochemical composition from three sites in the W Mediterranean (Ebro river coast, bays of Almería and Málaga

    Directory of Open Access Journals (Sweden)

    Alberto Garcia

    2006-10-01

    Full Text Available Late larval stages of sardine (16-23 mm were sampled during the 2003 spawning season in their nursery grounds located off the Ebro river mouth, on the Catalan coast, and in two bays of the Alborán Sea coasts, the Bay of Almería and the Bay of Málaga. The daily growth analysis of each sampled population revealed faster growth in the Ebro sardine larvae than in both of the Alboran Sea larval populations. This fact is supported by their greater content with age of DNA, RNA and protein. However, the significantly higher carbohydrate content of the Bay of Almería sardine larvae and a higher Fulton’s index, indicative of energy storage of individuals, in both of the sardine populations sampled in the Bays of Almería and Málaga show evident differences in the daily growth of the Alborán Sea larvae from those originating in the Ebro region. Late larval growth in the Alborán Sea sardine tends to favour an increase in body mass rather than in body length. This study hypothesises that the productivity pulses off the Alboran Sea coasts induced by the north and northwestern wind regimes may be responsible for the growth pulses observed in the otolith microstructure.

  6. What can otolith shape analysis tell us about population structure of the European sardine, Sardina pilchardus, from Atlantic and Mediterranean waters?

    Science.gov (United States)

    Jemaa, Sharif; Bacha, Mahmoud; Khalaf, Gaby; Dessailly, David; Rabhi, Khalef; Amara, Rachid

    2015-02-01

    The European sardine, Sardina pilchardus, exhibits a complex population structure, which has produced conflicting results in previous genetic studies. Despite its importance in the fisheries industry, stock delineation for management and conservation purposes is still a matter of debate throughout the distribution range of the species. This study examines whether otolith shapes are more efficient than genetic markers to detect population structure in pelagic species with large population sizes. Sardines were analyzed from 15 sampling localities in the Northeast Atlantic and Mediterranean Sea covering almost the whole distribution range of the species. A combination of otolith shape indices and elliptic Fourier descriptors was investigated by multivariate statistical procedures. Within the studied area, three distinct groups were identified with an overall correct classification of 77%. Group A: northern Mediterranean Sea and Gulf of Gabès; group B: Atlantic Morocco-south Alboran-Algero-provençal coasts; and group C: European Atlantic coast. The Almeria-Oran front and the Gibraltar strait are not an efficient barrier for sardine population separation as there seems to be exchanges between populations of the south-western Mediterranean Sea and those of the Moroccan Atlantic Ocean coast or Gulf of Cadiz. The results are discussed in relation to environmental conditions, oceanographic features, and physical barriers to dispersal in the study area, and compared with those obtained by previous genetic, morphometric, and meristic data. For pelagic species with high gene flow, present results highlighted the need to take into account the identification of phenotypic stocks to ensure sustainable fishery benefits and efficient conservation as they may have unique demographic properties and responses to exploitation.

  7. Fish protein hydrolysate production from sardine solid waste by crude pepsin enzymatic hydrolysis in a bioreactor coupled to an ultrafiltration unit

    Energy Technology Data Exchange (ETDEWEB)

    Benhabiles, M.S.; Abdi, N. [National Polytechnic school of Algiers, B.P. 182-16200, El Harrach, Algiers (Algeria); Drouiche, N., E-mail: nadjibdrouiche@yahoo.fr [National Polytechnic school of Algiers, B.P. 182-16200, El Harrach, Algiers (Algeria); Silicon Technology Development Unit (UDTS) 2, Bd Frantz Fanon BP140, Alger-7 Merveilles, 16000 (Algeria); Lounici, H. [National Polytechnic school of Algiers, B.P. 182-16200, El Harrach, Algiers (Algeria); Pauss, A. [University of Technology of Compiegne, Departement Genie chimique,B.P. 20.509, 60205 Compiegne cedex (France); Goosen, M.F.A. [Alfaisal University, Riyadh (Saudi Arabia); Mameri, N. [University of Technology of Compiegne, Departement Genie chimique,B.P. 20.509, 60205 Compiegne cedex (France)

    2012-05-01

    The aims of the study were to optimize the production a fish protein hydrolysate (FPH) by enzymatic hydrolysis of sardine solid waste using crude pepsin, and to scale up the process in a bioreactor coupled to an ultrafiltration unit for product recovery. Results showed that the crude pepsin prepared by autolysis of the mucous membranes of a sheep stomach at optimal conditions (i. e. pH = 1.5-2 and incubation time of 6 h) could be satisfactory used for the enzymatic hydrolysis of fish solid waste. The optimal conditions for enzymatic reaction were: temperature 48 Degree-Sign C, and pH 1.5. The scale up of the enzymatic hydrolysis and the coupling of the reactor an ultrafiltration unit to concentrate the hydrolysate gave good results with a rejection coefficient for the protein hydrolysate product in the range of 90%. The volumetric concentration factor was 2.5, with a permeate flux of 200 L m{sup -2} bar{sup -1}. However, the results also suggest that the ultrafiltration product concentration process may be operating beyond the critical flux at which point irreversible membrane fouling occurs. - Highlights: Black-Right-Pointing-Pointer Evaluating to produce a (FPH) by enzymatic hydrolysis of sardine solid wastes was achieved. Black-Right-Pointing-Pointer Investigation of key parameters for optimal conditions for enzymatic hydrolysis have been studied. Black-Right-Pointing-Pointer Valorization of sardine waste was realized by enzymatic hydrolysis process. Black-Right-Pointing-Pointer Performances of this enzyme gave comparable results to those obtained with commercial pepsin. Black-Right-Pointing-Pointer The nutritional quality of the FPH produced appears to be satisfactory.

  8. Stomach Chitinase from Japanese Sardine Sardinops melanostictus: Purification, Characterization, and Molecular Cloning of Chitinase Isozymes with a Long Linker.

    Science.gov (United States)

    Kawashima, Satoshi; Ikehata, Hiroki; Tada, Chihiro; Ogino, Tomohiro; Kakizaki, Hiromi; Ikeda, Mana; Fukushima, Hideto; Matsumiya, Masahiro

    2016-01-20

    Fish express two different chitinases, acidic fish chitinase-1 (AFCase-1) and acidic fish chitinase-2 (AFCase-2), in the stomach. AFCase-1 and AFCase-2 have different degradation patterns, as fish efficiently degrade chitin ingested as food. For a comparison with the enzymatic properties and the primary structures of chitinase isozymes obtained previously from the stomach of demersal fish, in this study, we purified chitinase isozymes from the stomach of Japanese sardine Sardinops melanostictus, a surface fish that feeds on plankton, characterized the properties of these isozymes, and cloned the cDNAs encoding chitinases. We also predicted 3D structure models using the primary structures of S. melanostictus stomach chitinases. Two chitinase isozymes, SmeChiA (45 kDa) and SmeChiB (56 kDa), were purified from the stomach of S. melanostictus. Moreover, two cDNAs, SmeChi-1 encoding SmeChiA, and SmeChi-2 encoding SmeChiB were cloned. The linker regions of the deduced amino acid sequences of SmeChi-1 and SmeChi-2 (SmeChi-1 and SmeChi-2) are the longest among the fish stomach chitinases. In the cleavage pattern groups toward short substrates and the phylogenetic tree analysis, SmeChi-1 and SmeChi-2 were classified into AFCase-1 and AFCase-2, respectively. SmeChi-1 and SmeChi-2 had catalytic domains that consisted of a TIM-barrel (β/α)₈-fold structure and a deep substrate-binding cleft. This is the first study showing the 3D structure models of fish stomach chitinases.

  9. Effects of sardine-enriched diet on metabolic control, inflammation and gut microbiota in drug-naïve patients with type 2 diabetes: a pilot randomized trial.

    Science.gov (United States)

    Balfegó, Mariona; Canivell, Silvia; Hanzu, Felicia A; Sala-Vila, Aleix; Martínez-Medina, Margarita; Murillo, Serafín; Mur, Teresa; Ruano, Elena G; Linares, Francisca; Porras, Nuria; Valladares, Silvia; Fontalba, Maria; Roura, Elena; Novials, Anna; Hernández, Cristina; Aranda, Gloria; Sisó-Almirall, Antoni; Rojo-Martínez, Gemma; Simó, Rafael; Gomis, Ramon

    2016-04-18

    Nutrition therapy is the cornerstone of treating diabetes mellitus. The inclusion of fish (particularly oily fish) at least two times per week is recommended by current international dietary guidelines for type 2 diabetes. In contrast to a large number of human studies examining the effects of oily fish on different cardiovascular risk factors, little research on this topic is available in patients with type 2 diabetes. The aims of this pilot study were to investigate the effects of a sardine-enriched diet on metabolic control, adiponectin, inflammatory markers, erythrocyte membrane fatty acid (EMFA) composition, and gut microbiota in drug-naïve patients with type 2 diabetes. 35 drug-naïve patients with type 2 diabetes were randomized to follow either a type 2 diabetes standard diet (control group: CG), or a standard diet enriched with 100 g of sardines 5 days a week (sardine group: SG) for 6 months. Anthropometric, dietary information, fasting glycated hemoglobin, glucose, insulin, adiponectin, inflammatory markers, EMFA and specific bacterial strains were determined before and after intervention. There were no significant differences in glycemic control between groups at the end of the study. Both groups decreased plasma insulin (SG: -35.3%, P = 0.01, CG: -22.6%, P = 0.02) and homeostasis model of assessment--insulin resistance (HOMA-IR) (SG: -39.2%, P = 0.007, CG: -21.8%, P = 0.04) at 6-months from baseline. However only SG increased adiponectin in plasma compared to baseline level (+40.7%, P = 0.04). The omega-3 index increased 2.6% in the SG compared to 0.6% in the CG (P = 0.001). Both dietary interventions decreased phylum Firmicutes (SG and CG: P = 0.04) and increased E. coli concentrations (SG: P = 0.01, CG: P = 0.03) at the end of the study from baseline, whereas SG decreased Firmicutes/Bacteroidetes ratio (P = 0.04) and increased Bacteroides-Prevotella (P = 0.004) compared to baseline. Although enriching diet with 100 g of sardines 5 days a week during

  10. Effect of different types of coatings on texture and nutritional properties of canned Portuguese sardines (Sardina pilchardus)

    OpenAIRE

    Rita Pinheiro; Manuela Vaz Velho

    2014-01-01

    Canned foods have a long history of success and are likely to remain popular for the foreseeable future owing to their convenience, long shelf life and economy. Interest in fish consumption has increased in recent years due to the wide range of its health benefits. In addition to fresh product, canned fish enables a delayed consumption of this appreciated kind of food. Among the different types of fish, in Portugal, the most used in canning manufacture are sardines, tuna, anchovies, mackerels. Ve...

  11. Effect of Mentha spicata L. and Artemisia campestris extracts on the shelf life and quality of vacuum-packed refrigerated sardine (Sardina pilchardus) fillets.

    Science.gov (United States)

    Houicher, Abderrahmane; Kuley, Esmeray; Bendeddouche, Badis; Ozogul, Fatih

    2013-10-01

    The present study investigated the effects of ethanolic extracts obtained from Mentha spicata and Artemisia campestris on the shelf life and the quality of vacuum-packed sardine fillets stored at 3 ± 1°C for a period of 21 days. The three groups were tested were VC, control group; VM, group treated with 1 % mint extract; and VA, group treated with 1 % artemisia extract. The observed shelf life of sardine fillets was 10 days for control samples, whereas the combination of vacuum packaging with mint and artemisia extracts extended the product's shelf life to 17 days. Among the chemical indices determined, the thiobarbituric acid-reactive substances values were significantly lower in VM samples. Total volatile base nitrogen was maintained at low levels in VA samples until 17 days of chilled storage. Results of aerobic plate counts and coliform counts showed the existence of a reduced growth in VA group, whereas lactic acid bacteria did not show a significant difference among groups. Natural extract treatments combined with vacuum packaging showed lower microbiological and chemical indices, indicating that the presence of phenolic compounds in mint and artemisia extracts and the removal of oxygen in the pack retarded lipid oxidation and reduced the growth of microorganisms, which resulted in preventing spoilage and extending the product's shelf life.

  12. The effect of combined traditional and novel treatments on oxidative status of dolphinfish (Coryphaena hippurus) and sardine (Sardina pilchardus) muscle lipids.

    Science.gov (United States)

    Gómez-Estaca, Joaquín; Gómez-Guillén, M Carmen; Montero, Pilar

    2014-09-01

    Fish is rich in polyunsaturated fatty acids with beneficial effects on human health; however, these lipids are very sensitive to auto-oxidation reactions, leading to loss of nutritional and sensory quality. The effect of traditional (brining, smoking) and novel (addition of polyphenolic extracts, high pressure) preservation processes on the antioxidant/oxidative status of muscle lipids from dolphinfish and sardine was studied. Brining with oregano or rosemary aqueous extracts, as well as smoking, gave rise to deposition of phenolic compounds (9-42, 1.5-4.5 and 0.4-2.3 µg phenol/g for smoked, oregano-brined and rosemary-brined samples, respectively) in the muscle of both fish species. The antioxidant activity, as measured by ferric reducing ability, was also improved after brining with antioxidant extracts or smoking, results ranging from 8.9 to 82 mM FeSO4 · 7H2O equivalents/mg muscle depending on the treatment and the fish species. Consequently, fish lipid oxidation (as measured by thiobarbituric acid reactive substances) derived from brining and pressurizing, applied alone or in combination, was reduced between 6.6 and 69.8% depending on the treatment and the fish species. The combination of brining with oregano extract and light smoking showed an additional antioxidant effect, as compared with that obtained by smoking, on reducing sardine lipid oxidation derived from brining and pressurizing.

  13. Preparation of triacylglycerols rich in omega-3 fatty acids from sardine oil using a Rhizomucor miehei lipase: focus in the EPA/DHA ratio.

    Science.gov (United States)

    Bispo, Paulo; Batista, Irineu; Bernardino, Raul J; Bandarra, Narcisa Maria

    2014-02-01

    The increasing evidence on the differential biochemical effects of eicosapentaenoic acid (EPA)/docosahexaenoic acid (DHA) raises the need of n-3 highly unsaturated fatty acid concentrates with different amounts of these fatty acids. In the present work, physicochemical and enzymatic techniques were combined to obtain acylglycerols, mainly triacylglycerols (TAG), rich in n-3 fatty acids. Sardine oil was obtained by washing sardine (Sardina pilchardus) mince with a NaHCO3 solution, hydrolyzed in a KOH-ethanol solution, and concentrated with urea. The esterification reaction was performed in the stoichiometric proportion of substrates for re-esterification to TAG, with 10 % level of Rhizomucor miehei lipase based on the weight of substrates, without any solvent, during 48 h. This procedure led to approximately 88 % of acylglycerols, where more than 66 % were TAG and the concentration of n-3 fatty acids was higher than 60 %, the EPA and DHA ratio (EPA/DHA) was 4:1. The content of DHA in the unesterifed fraction (free fatty acids) increased from 20 to 54 %, while the EPA level in the same fraction decreased from 33 to 12.5 % (EPA/DHA ratio ≈1:4). Computational methods (density functional theory calculations) have been carried out at the B3LYP/6-31G(d,p) level to explain some of the experimental results.

  14. [The plate in the zone of oocyte and germinal epithelium contact in scyphomedusa Aurelia aurita binds antibodies to ZP-domain-containing protein mesoglein].

    Science.gov (United States)

    Adonin, L S; Podgornaia, O I; Matveev, I V; Shaposhnikova, T G

    2009-01-01

    Cnidaria are lower multicellular animals with the body consisting of two epithelial layers. An extracellular substance--mesoglea--is situated between epidermal and gastrodermal layers of these animals. Mesoglein is one of the major mesogleal proteins of adult medusa of Scyphozoan jellyfish Aurelia aurita. Search for the known domains in mesoglein amino acid sequence reveals prominent zona pellucida (ZP) domain (which was found at first in the mammal oocyte zona pellucida proteins), so the protein belongs to ZP family of extracellular matrix proteins and it is an early metazoan member of ZP-domain-containing protein family. However, nothing is known about oogenesis related ZP-domain proteins in the lower multicellular animals. Oogenesis in Scyphozoa is described poorly. In this work morphological features of the zone in contact area between the oocyte and the germinal epithelium were investigated in semi-fine sections: To make it more convenient we identified seven stages according to the oocyte size and the structure found in this area was named the plate. It was shown that the components of the plate bound specifically the antibodies against mesoglein. So it seems the plate material contains ZP-domain proteins. Electrophoresis and immunoblot results give evidence that the proteins immunologically related to mesoglein have a higher molecular mass. It might be due to either the posttranslational modifications of the precursors or that they represent other proteins of ZP-domain family in Cnidaria.

  15. The use of ephyrae of a scyphozoan jellyfish, Aurelia aurita, in the aquatic toxicological assessment of Macondo oils from the Deepwater Horizon incident.

    Science.gov (United States)

    Echols, B S; Smith, A J; Gardinali, P R; Rand, G M

    2016-02-01

    Ephyrae of the scyphozoan jellyfish, Aurelia aurita, were evaluated in 96-hr acute toxicity tests for lethal response to Macondo crude oils from the Deepwater Horizon (DWH) incident in the Gulf of Mexico (GOM), Corexit 9500, and oil-dispersant mixtures. Water accommodated fractions (WAFs) of weathered and unweathered Macondo crude oils were not acutely toxic to ephyrae (LC50s > 100% WAF). The total PAHs (TPAHs), measured as the sum of 46 PAHs, averaged 21.1and 152 µg TPAH/L for WAFs of weathered and unweathered oil, respectively. Mortality was significantly (p = <0.0001) higher in the three highest exposure concentrations (184-736 µg TPAH/L) of chemically dispersed WAFs (CEWAF) compared to controls. Dispersant only tests resulted in a mean LC50 of 32.3 µL/L, which is in the range of previously published LC50s for marine zooplankton. Changes in appearance and muscle contractions were observed in organisms exposed to CEWAF dilutions of 12.5 and 25%, as early as 24 h post-exposure. Based on the results of these tests, crude oil alone did not cause significant acute toxicity; however, the presence of chemical dispersant resulted in substantial mortality and physical and behavioral abnormalities either due to an increase in hydrocarbons or droplet exposure. Copyright © 2015 Elsevier Ltd. All rights reserved.

  16. Where are the polyps? Molecular identification, distribution and population differentiation of Aurelia aurita jellyfish polyps in the southern North Sea area.

    Science.gov (United States)

    van Walraven, Lodewijk; Driessen, Floor; van Bleijswijk, Judith; Bol, Anneke; Luttikhuizen, Pieternella C; Coolen, Joop W P; Bos, Oscar G; Gittenberger, Adriaan; Schrieken, Niels; Langenberg, Victor T; van der Veer, Henk W

    For many species of metagenic jellyfish the location of the benthic polyps is unknown. To gain insight in the distribution, species composition and population structure of scyphozoan jellyfish polyps in the southern North Sea area, polyp samples were collected from natural and artificial substrates (settling plates, marina floats and wrecks) at ten inshore locations in the Netherlands, seven offshore locations in the North Sea and in the Gullmar Fjord in Sweden. Polyps were identified to species level by sequencing both a fragment of 18S rDNA and a fragment of mitochondrial COI, and comparing these sequences to reference sequences available in GenBank and to newly obtained sequences from medusae collected in the area. All polyps sequenced did belong to Aurelia aurita. For this species, molecular diversity in mitochondrial COI was high, with 50 haplotypes among 183 polyps. Population differentiation was detected between the Dogger Bank and other-more coastal-locations, indicating extremely low connectivity. No significant differences were found between coastal samples. The location of polyps of Cyanea capillata, Cyanea lamarckii, Chrysaora hysoscella and Rhizostoma octopus in the study area remains unresolved.

  17. A newly discovered oxidant defence system and its involvement in the development of Aurelia aurita (Scyphozoa, Cnidaria): reactive oxygen species and elemental iodine control medusa formation.

    Science.gov (United States)

    Berking, Stefan; Czech, Nicole; Gerharz, Melanie; Herrmann, Klaus; Hoffmann, Uwe; Raifer, Hartmann; Sekul, Guy; Siefker, Barbara; Sommerei, Andrea; Vedder, Fritz

    2005-01-01

    In Aurelia aurita, applied iodine induces medusa formation (strobilation). This process also occurs when the temperature is lowered. This was found to increase oxidative stress resulting in an increased production of iodine from iodide. One polyp produces several medusae (initially termed ephyrae) starting at the polyp's oral end. The spreading of strobilation down the body column is controlled by a feedback loop: ephyra anlagen decrease the tyrosine content in adjacent polyp tissue by producing melanin from tyrosine. Endogenous tyrosine is able to remove iodine by forming iodiferous tyrosine compounds. The reduced level of tyrosine causes the ephyra-polyp-border to move towards the basal end of the former polyp. We argue that an oxidant defence system may exist which makes use of iodide and tyrosine. Like other marine invertebrates, polyps of Aurelia contain iodide ions. Inevitably produced peroxides oxidise iodide into iodine. The danger to be harmed by iodine is strongly decreased by endogenous tyrosine which reacts with iodine to form iodiferous tyrosine compounds including thyroxin. Both substances together, iodide and tyrosine, form an efficient oxidant defence system which shields the tissue against damage by reactive oxygen species. In the course of evolution (from a species at the basis of the animal kingdom like Aurelia to a highly evolved species like man) the waste product thyroxin (indicating a high metabolic rate) has developed into a hormone which controls the metabolic rate.

  18. Functional and antioxidant properties of hydrolysates of sardine (S. pilchardus) and horse mackerel (T. mediterraneus) for the microencapsulation of fish oil by spray-drying.

    Science.gov (United States)

    Morales-Medina, R; Tamm, F; Guadix, A M; Guadix, E M; Drusch, S

    2016-03-01

    The functionality of fish protein hydrolysates (FPH) for the microencapsulation of fish oil was investigated. Muscle protein from sardine (Sardina pilchardus) and horse mackerel (Trachurus mediterraneus) was hydrolysed using Alcalase or trypsin. Physically stable emulsions suitable for spray-drying were obtained when using FPH with a degree of hydrolysis of 5%. Microencapsulation efficiency amounted to 98±0.1% and oxidative stability of the encapsulated oil over a period of twelve weeks was in a similar range as it is reported for other matrix systems. Therefore, the suitability of FPH for use in spray-dried emulsions has been shown for the first time. Since no clear correlation between the antioxidative activity of the FPH and the course of lipid oxidation could be established future research is required to more specifically characterise the molecular structure of the peptides and its impact on protein alteration and role in lipid oxidation.

  19. Sardine protein diet increases plasma glucagon-like peptide-1 levels and prevents tissue oxidative stress in rats fed a high-fructose diet.

    Science.gov (United States)

    Madani, Zohra; Sener, Abdullah; Malaisse, Willy J; Dalila, Ait Yahia

    2015-11-01

    The current study investigated whether sardine protein mitigates the adverse effects of fructose on plasma glucagon‑like peptide-1 (GLP-1) and oxidative stress in rats. Rats were fed casein (C) or sardine protein (S) with or without high‑fructose (HF) for 2 months. Plasma glucose, insulin, GLP‑1, lipid and protein oxidation and antioxidant enzymes were assayed. HF rats developed obesity, hyperglycemia, hyperinsulinemia, insulin resistance and oxidative stress despite reduced energy and food intakes. High plasma creatinine and uric acid levels, in addition to albuminuria were observed in the HF groups. The S‑HF diet reduced plasma glucose, insulin, creatinine, uric acid and homeostasis model assessment‑insulin resistance index levels, however increased GLP‑1 levels compared with the C‑HF diet. Hydroperoxides were reduced in the liver, kidney, heart and muscle of S‑HF fed rats compared with C‑HF fed rats. A reduction in liver, kidney and heart carbonyls was observed in S‑HF fed rats compared with C‑HF fed rats. Reduced levels of nitric oxide (NO) were detected in the liver, kidney and heart of the S‑HF fed rats compared with C‑HF fed rats. The S diet compared with the C diet reduced levels of liver hydroperoxides, heart carbonyls and kidney NO. The S‑HF diet compared with the C‑HF diet increased the levels of liver and kidney superoxide dismutase, liver and muscle catalase, liver, heart and muscle glutathione peroxidase and liver ascorbic acid. The S diet prevented and reversed insulin resistance and oxidative stress, and may have benefits in patients with metabolic syndrome.

  20. Changes in bacterial community metabolism and composition during the degradation of dissolved organic matter from the jellyfish Aurelia aurita in a Mediterranean coastal lagoon.

    Science.gov (United States)

    Blanchet, Marine; Pringault, Olivier; Bouvy, Marc; Catala, Philippe; Oriol, Louise; Caparros, Jocelyne; Ortega-Retuerta, Eva; Intertaglia, Laurent; West, Nyree; Agis, Martin; Got, Patrice; Joux, Fabien

    2015-09-01

    Spatial increases and temporal shifts in outbreaks of gelatinous plankton have been observed over the past several decades in many estuarine and coastal ecosystems. The effects of these blooms on marine ecosystem functioning and particularly on the dynamics of the heterotrophic bacteria are still unclear. The response of the bacterial community from a Mediterranean coastal lagoon to the addition of dissolved organic matter (DOM) from the jellyfish Aurelia aurita, corresponding to an enrichment of dissolved organic carbon (DOC) by 1.4, was assessed for 22 days in microcosms (8 l). The high bioavailability of this material led to (i) a rapid mineralization of the DOC and dissolved organic nitrogen from the jellyfish and (ii) the accumulation of high concentrations of ammonium and orthophosphate in the water column. DOM from jellyfish greatly stimulated heterotrophic prokaryotic production and respiration rates during the first 2 days; then, these activities showed a continuous decay until reaching those measured in the control microcosms (lagoon water only) at the end of the experiment. Bacterial growth efficiency remained below 20%, indicating that most of the DOM was respired and a minor part was channeled to biomass production. Changes in bacterial diversity were assessed by tag pyrosequencing of partial bacterial 16S rRNA genes, DNA fingerprints, and a cultivation approach. While bacterial diversity in control microcosms showed little changes during the experiment, the addition of DOM from the jellyfish induced a rapid growth of Pseudoalteromonas and Vibrio species that were isolated. After 9 days, the bacterial community was dominated by Bacteroidetes, which appeared more adapted to metabolize high-molecular-weight DOM. At the end of the experiment, the bacterial community shifted toward a higher proportion of Alphaproteobacteria. Resilience of the bacterial community after the addition of DOM from the jellyfish was higher for metabolic functions than diversity

  1. The fate of eggs and larvae of three pelagic species, mackerel (Scomber scombrus, horse mackerel (Trachurus trachurus and sardine (Sardina pilchardus in relation to prevailing currents in the Bay of Biscay: Could they affect larval survival?

    Directory of Open Access Journals (Sweden)

    Paula Alvarez

    2012-05-01

    Full Text Available The spatial distribution of eggs and larvae of three pelagic species, mackerel, horse mackerel and sardine, in the Bay of Biscay was studied in 1998, 2001 and 2004. The spatial distribution was clearly different between the years studied and corresponds quite precisely to different water circulation regimes. Mackerel and horse mackerel larvae are more affected by the prevailing currents than sardine, as their spawning grounds are located offshore, far from the shelf break where the current velocities are higher. Survival rates for mackerel and horse mackerel were higher in 2001, when the offshore larval transport was stronger. However, for sardine, the mortality rate hardly varied between years. The abundance of 25-day-old larvae, considered as an index of the survival rate, appears to be a good recruitment indicator, at least for 1998, 2001 and 2004. Our results did not support the hypothesis of Bakun (1996, which states that dispersion of early life stages towards open ocean waters should cause high larval mortality. At least under the conditions observed for the years studied, the retention of larvae offshore appears to have a positive effect on larval growth and/or survival.

  2. Variation in the parasite community of the sardine fish Triportheus nematurus (Actinopterygii: Characidae) from the Medalha lagoon in the Pantanal wetland, Brazil.

    Science.gov (United States)

    Costa-Pereira, R; Paiva, F; Tavares, L E R

    2014-09-01

    In July 2009 and July 2010 (two dry periods separated by an atypically large flood in the Pantanal wetland of Brazil), 34 and 33 specimens of the sardine fish Triportheus nematurus were collected, respectively, for the study of the metazoan parasite community of this species. Parasite ecological and community descriptors were calculated for both host samples, and possible similarities were tested statistically. Five species of metazoan parasites were identified, four of which were common to both host samples. A total of 61 metazoan parasites were collected from all fish hosts (17 specimens in July 2009 (mean: 0.5 ± 0.66 parasites/fish) and 44 specimens in July 2010 (mean: 1.33 ± 1.41 parasites/fish)). The nematode Procamallanus hilarii and the monogenean Anacanthorus sp. were the most prevalent and abundant species in 2009 and 2010, respectively. The mean total abundance and species richness were significantly higher in 2010. Parasite communities in both samples of T. nematurus were characterized by species with low prevalence, abundance, mean total abundance and species richness, thus indicating low parasite diversity. Significant differences in the prevalence and abundance of P. hilarii and Anacanthorus sp. between the two samples allowed the discrimination of infracommunities, which were united in two distinct groups. This appears to be the first evidence that the peculiar hydrological dynamics of the southern Pantanal wetland (Brazil) exert an important influence over the structure of the parasite community.

  3. The commercial fishing of sardines (Triportheus spp. landed in the fishery market of Porto Velho, Rondônia (1990-2004: Yield and general profile

    Directory of Open Access Journals (Sweden)

    Luiz Jardim de Queiroz

    2008-09-01

    Full Text Available The profile of sardine fishing, the Triportheus species, landed at the fishing market of Porto Velho (RO between 1990 and 2004, was established by analyzing variations in the intra- and inter-annual yield as well as the length and general characteristics of the fishing. The annual production varied from 15,372 to 78,340kg (average = 33,482kg, totaling 5.46% of the overall production during the studied period. These variations can be related to the changes in the fishermen’s effort, as a reflex to searching for alternatives for fishing, or related to the level of the river, since the greatest annual yields were preceded by years of having more intense flooding. The monthly production varied from 0 to 21,456kg (average=2,876kg. Greater values of production were obtained in periods of lower hydrological levels (dry season [r(Pearson= -0.53. p<0.0001]. The average capture per fisherman/day between 2003 and 2004 was 9.27kg. Rivers were the main environment from which the captures took place, mainly from the Jamari, Machado and Novo Aripuanã rivers, that are tributaries of the Madeira River. The lengths of the fishes varied from 70 to 380mm and presented a bimodal pattern, suggesting the presence of more than one species within the captures.

  4. Estudos sobre estrutura, ciclo de vida e comportamento de Sardinella brasiliensis (Steindachner, 1879, na área entre 22ºS e 28ºS, Brasil: 3. morfologia e histologia de ovários e escala de maturidade Studies on the structure, life cycle and behaviour of Sardinella brasiliensis (Steindachner, 1879, in the region between 22ºS and 28ºS, Brazil: 3. Morphology and histology of ovaries and maturity scale

    Directory of Open Access Journals (Sweden)

    Victoria Judith Isaac-Nahum

    1983-01-01

    Full Text Available Uma melhor compreensão da dinâmica do processo reprodutivo de Sardinella brasiliensis e obtida através da associação de aspectos micro e macroscópicos das gonadas durante o ciclo sexual. O presente trabalho apresenta uma descrição das fases de desenvolvimento ovocitarias e dos estadios de maturidade dos ovarios de 119 fêmeas de S. brasiliensis, coletados na costa brasileira entre 22ºS e 28ºS. São descritas seis fases ovocitarias e duas estruturas derivadas, analisando-se as características citologicas das células germinativas durante o processo de maturação. Foram estabelecidos sete estadios: A (virgem, Bi (em início de maturação, Bf (em maturação avançada, Cf (maduro inicial, Cf (maduro desovado, D (es vaziado e R (em repouso, sendo discutida sua correspondência com outras es calas aplicadas as espécies.A better understanding of the reproductive process is obtained when histologic analysis of gonades is associated with macroscopic observations during the sexual cycles. This paper describes the developmental stages of the oocytes of the ovaries in 119 females of S. brasiliensis sampled along the Brazilian coast, between 22ºS and 28ºS. The cytologic characteristics of the germinative cells during the maturation process are given and six oocyte phases are described. Seven maturity stages are established: A (virgin, Bi (incipient maturation, Bf (advanced maturation, Ci (mature, Cf (ripe, D (spent and R (repose. These stages are compared to those presently used with respect to S. brasiliensis.

  5. Obtention of highly purified fractions of eicosapentaenoic acid and docosahexaenoic acid from sardine oil by silver-resin chromatography. A semipreparative procedure.

    Directory of Open Access Journals (Sweden)

    Nieto, S.

    1997-08-01

    Full Text Available Pure fractions of eicosapentaenoic acid (ERA and docosahexaenoic acid (DHA were obtained from silver-resin column chromatography of ERA+DHA concentrates obtained from sardine oil. Two types of silver-impregnated resins were assayed; Amberlite IR-118H and Dowex 50 W-HCR-W2.The Amberlite-silver column allows the separation of almost pure fractions (98.5% of ERA, being unable to separate DHA from other polyunsaturated fatty acids. The Dowex-silver column does not separate the ERA from the concentrate but allows the isolation of a highly purified fraction of DHA. The chromatographic procedure described may provide pure forms of two important n-3 polyunsaturated fatty acids for nutritional or pharmacological research or as substrates for the obtention of structured lipids.

    Mediante cromatografía en columna utilizando resinas impregnadas con ion plata se separaron fracciones puras de ácido eicosapentaenoico (EPA y ácido docosahexaenoico (DHA a partir de un concentrado de EPA+DHA obtenido de aceite de sardina. Se utilizaron dos tipos de resinas impregnadas con plata: Amberlite-IR 118H y Dowex 50 W-HCR-W2. La columna de Amberlite-plata permite la separación de fracciones casi puras de ERA (98,5%, siendo incapaz de separar el DHA de otros ácidos grasos poliinsaturados. La columna de Dowex-plata no separa el ERA a partir del concentrado, pero permite la obtención de una fracción de DHA de alta pureza. El procedimiento cromatográfico que se describe permite obtener fracciones puras de dos importantes ácidos grasos poliinsaturados de la serie n-3 que pueden ser utilizados para la investigación nutricional o farmacológica, o como sustratos para la obtención de lípidos estructurados.

  6. INTERESTERIFIKASI ENZIMATIS PALM STEARIN DAN MINYAK IKAN LEMURU UNTUK MEMBUAT LEMAK MARGARIN [Enzymatic Interesterification of Palm Stearin and Sardine oil to Produce Margarine-fat

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    Pudji Hastuti

    2003-04-01

    Full Text Available Enzymatic interesterification of Palm Stearin (PS and Sardine Oil (SO as source of Eicosa Pentaenoic Acid (EPA and Docosa Hexaenoic Acid (DHA have been of interest to modify the physical properties of the triglyceride. An attempt to enzymatic-restructure PS and SO to form Structured Lipid (SL which is suitable for margarine was investigated using immobilized lipase from Rhizomucor miehei and that from Candida antartica. The effect of reaction time course, ratio of PS/SO and ratio of enzyme/substrate were studied in the present study. At the end of interesterification, the enzyme was filtered from the reaction mixture through a filter paper. The Solid Fat Index (SFI was determined by dillatometry. The Slip Melting Point (SMP was determined by capillary tube method. Both of interesterification catalyzed by immobilized sn 1,3 specific lipase from R.miehei,and non specific lipase from C.antartica were found to decrease the SFI value at 10; 21.1 and 33.3°C. The SMP value was decrease from 58-50°C to 37-39°C. The change of these parameters were slightly faster in the reaction which catalyzed by lipase from R miehei than lipase from C.antartica . The more the utilization of the enzyme the faster the change were occurred, especially the increase of enzyme utilization from 2.5% to 5%, which decrease the SFI value at 33,30C. The decrease of the PS/SO ratio resulted in the decrease of SFI and SMP values. It was found that the most suitable SFI and SMP value for margarine fat is the SL formed by carrying out the enzymatic-interesterification of PS/SO with the ratio of 40/60 using enzyme 2.5% of the total fat, for 8 hours at 60°C.

  7. Effect of sardine proteins on hyperglycaemia, hyperlipidaemia and lecithin:cholesterol acyltransferase activity, in high-fat diet-induced type 2 diabetic rats.

    Science.gov (United States)

    Benaicheta, Nora; Labbaci, Fatima Z; Bouchenak, Malika; Boukortt, Farida O

    2016-01-14

    Type 2 diabetes (T2D) is a major risk factor of CVD. The effects of purified sardine proteins (SP) were examined on glycaemia, insulin sensitivity and reverse cholesterol transport in T2D rats. Rats fed a high-fat diet (HFD) for 5 weeks, and injected with a low dose of streptozotocin, were used. The diabetic rats were divided into four groups, and they were fed casein (CAS) or SP combined with 30 or 5% lipids, for 4 weeks. HFD-induced hyperglycaemia, insulin resistance and hyperlipidaemia in rats fed HFD, regardless of the consumed protein. In contrast, these parameters lowered in rats fed SP combined with 5 or 30% lipids, and serum insulin values reduced in SP v. CAS. HFD significantly increased total cholesterol and TAG concentrations in the liver and serum, whereas these parameters decreased with SP, regardless of lipid intake. Faecal cholesterol excretion was higher with SP v. CAS, combined with 30 or 5% lipids. Lecithin:cholesterol acyltransferase (LCAT) activity and HDL3-phospholipids (PL) were higher in CAS-HF than in CAS, whereas HDL2-cholesteryl esters (CE) were lower. Otherwise, LCAT activity and HDL2-CE were higher in the SP group than in the CAS group, whereas HDL3-PL and HDL3-unesterified cholesterol were lower. Moreover, LCAT activity lowered in the SP-HF group than in the CAS-HF group, when HDL2-CE was higher. In conclusion, these results indicate the potential effects of SP to improve glycaemia, insulin sensitivity and reverse cholesterol transport, in T2D rats.

  8. Identification document for large by-catch species in the Mauritanian Exclusive Economic Zone

    NARCIS (Netherlands)

    Hofstede, ter R.

    2003-01-01

    The coastal waters of Mauritania (Northwest Africa) contain large numbers of pelagic fish such as sardinella, sardine, mackerel and horse mackerel. Freezer-trawlers from the European Union (EU), mainly of Dutch origin, have exploited these resources since 1996. In 1998 the Dutch ship owners commissi

  9. Identification document for large by-catch species in the Mauritanian Exclusive Economic Zone

    NARCIS (Netherlands)

    Hofstede, ter R.

    2003-01-01

    The coastal waters of Mauritania (Northwest Africa) contain large numbers of pelagic fish such as sardinella, sardine, mackerel and horse mackerel. Freezer-trawlers from the European Union (EU), mainly of Dutch origin, have exploited these resources since 1996. In 1998 the Dutch ship owners

  10. Analysis of the morphology and distribution of argentaffin, argyrophil and insulin-immunoreactive endocrine cells in the small intestine of the adult opossum Didelphis aurita (Wied-Neuwied, 1826).

    Science.gov (United States)

    Basile, D R S; Novaes, R D; Marques, D C S; Fialho, M C Q; Neves, C A; Fonseca, C C

    2012-10-01

    The aim of this study was to identify and quantify the argyrophil, argentaffin and insulin-immunoreactive cells (IIC) in the small intestine of the opossum Didelphis aurita. Seven adult male specimens of opossums were investigated. The animals were captured, and their blood insulin levels were determined. After euthanasia, fragments of the small intestine were processed for light microscopy and transmission electron microscopy, and submitted to histochemistry and immunohistochemistry for identification of argyrophil and argentaffin endocrine cells, and IIC. Argyrophil and argentaffin cells were identified in the intestinal villi and Liberkühn crypts, whereas IIC were present exclusively in the crypts. Ultrastructure of the IIC revealed cytoplasmic granules of different sizes and electron densities. The numbers of IIC per mm(2) in the duodenum and jejunum were higher than in the ileum (psmall intestine. The IIC presented secretory granules, elongated and variable morphology. It is believed that insulin secretion by the IIC may influence the proliferation of cells in the Liberkühn crypts, and local glucose homeostasis, primarily in animals with low serum insulin levels, such as the opossum.

  11. Preliminary study on the competition in the larvae stage between Aurelia aurita and Rhopilema esculentum during a short term%海月水母与海蜇不同幼体阶段的种间短期竞争研究

    Institute of Scientific and Technical Information of China (English)

    迟旭朋; 游奎; 马彩华; 袁韵涵; 陈四淸; 杨叶蕃; 刘心田

    2013-01-01

    近年来海蜇池塘养殖业的兴起,补充了传统海蜇渔业捕捞生产的不足,推动了海蜇产业的迅猛发展。海月水母常常在海蜇养殖池塘中泛滥成灾,对海蜇养殖业常常造成很大损失,但关于海蜇与海月水母的种间竞争关系尚缺乏研究。本文对海月水母与海蜇的螅状体、碟状体及水母体幼体相互之间的短期生存竞争关系进行了初步观察研究。结果表明:除海月水母碟状体可以被海蜇螅状体所捕食外,各种类型的海蜇幼体基本不能对海月水母幼体造成危害。但海月水母螅状体及其水母体幼体对海蜇幼体均存在一定的危害性,尤其是对海蜇碟状体的危害性最为严重,在饵料缺乏的情况下海蜇碟状体可以100%地被捕食。随着海蜇幼体的生长,其对于海月水母危害的抵抗力逐渐增强。本研究显示海月水母相对于海蜇而言,在幼体种间竞争关系中处于绝对优势,这是海月水母时常暴发成灾的一个重要因素。在海蜇池塘养殖生产及苗种培育过程中,应当严禁混入海月水母幼体,以避免对海蜇养殖生产造成损害。%In recent years ,the rise of the jellyfish pond culture covers the decrease of the traditional jellyfish fisheries production in China .And jellyfish pond cultures develop rapidly with high profits . A urelia aurita is one of the most common jellyfish bloom species which usually occurs in the culture ponds of edible jellyfish Rhopilema escu-lentum and causes huge losses .However ,there is little research about the interspecific competition between A .au-rita and R .esculentum .In this paper ,a preliminary study was finished about the short-term survival and competi-tive relationship among the scyphistomae ,ephyrae and larvae of A .aurita and R .esculentum .The results are as follows:Only the ephyraes of A .aurita can be eaten by the scyphistomae of R .esculentum ,but the ephyrae and larvae of

  12. Utilization of wasted sardine oil as co-substrate with pig slurry for biogas production--a pilot experience of decentralized industrial organic waste management in a Portuguese pig farm.

    Science.gov (United States)

    Ferreira, L; Duarte, E; Figueiredo, D

    2012-07-01

    This work aimed to demonstrate in a pig farm and in real conditions, the possibilities to co-digest wasted sardine oil (WSO) and pig slurry (PS) at farm scale. A biogas mobile pilot plant, was set up in the farm and operated in real conditions during 4 months. Dynamic mesophilic (35-37 °C) continuous pilot trials were performed during four different periods of time. In each period a different organic loading rate (OLR) based on the chemical oxygen demand (COD) was operated sequentially, with pig slurry (PS) (OLR = 1.6 kg COD/m(3) d(-1)) and with mixtures of WSO:PS with a volumetric composition (% v/v) of 2:98 (OLR = 3.0 kg COD/m(3) d(-1)), 3:97 (OLR = 3.7 kg COD/m(3) d(-1)) and 5:95 (OLR = 5.2 kg COD/m(3) d(-1)). Biomass adapted very fast in metabolise the WSO and biogas productivity was raised substantially for different compositions of WSO:PS. Process stability indicators pH and Total volatile fatty acids/bicarbonate alkalinity (T-VFA/BA) ratio, suggests that the co-digestion process was robust. It was concluded that WSO could be easily co-digested in farm scale biogas plants. Copyright © 2012. Published by Elsevier Ltd.

  13. Isolation and genetic characterisation of Toxoplasma gondii from a red-handed howler monkey (Alouatta belzebul), a jaguarundi (Puma yagouaroundi), and a black-eared opossum (Didelphis aurita) from Brazil.

    Science.gov (United States)

    Pena, H F J; Marvulo, M F V; Horta, M C; Silva, M A; Silva, J C R; Siqueira, D B; Lima, P-A C P; Vitaliano, S N; Gennari, S M

    2011-02-10

    Toxoplasma gondii isolates are highly diverse in domestic animals from Brazil. However, little is known about the genetics of this parasite from wild mammals in the same region. Reveal genetic similarity or difference of T. gondii among different animal populations is necessary for us to understand transmission of this parasite. Here we reported isolation and genetic characterisation of three T. gondii isolates from wild animals in Brazil. The parasite was isolated by bioassay in mice from tissues of a young male red handed howler monkey (Alouatta belzebul), an adult male jaguarundi (Puma yagouaroundi), and an adult female black-eared opossum (Didelphis aurita). The monkey and the jaguarundi had inhabited the Zoo of Parque Estadual Dois Irmãos, Pernambuco State, Northeastern Brazil, for 1 year and 8 years, respectively. The wild black-eared opossum was captured in São Paulo State, Southeastern Brazil, and euthanised for this study because it was seropositive for T. gondii (titre 1:100 by the modified agglutination test, MAT). Ten PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) markers, SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico, were used to genotype the isolates. T. gondii was isolated from the brain and heart homogenate of the monkey, the muscle homogenate of the jaguarundi, and the heart homogenate of the black-eared opossum. This was the first isolation of T. gondii from a neotropical felid from Brazil. The isolate from the monkey (TgRhHmBr1) was not virulent in mice, whereas the isolates from the jaguarundi (TgJagBr1) and the black-eared opossum (TgOpBr1) were virulent in mice. The genotype of the isolate from the monkey has been identified in isolates from a goat and ten chickens in the same region of Brazil, suggesting that it may be a common lineage circulating in this region. The genotypes of the isolates from the jaguarundi and the black-eared opossum have not been previously reported. Although there

  14. The influence of different diets on growth performance in moon jellyfish Aurelia aurita%不同饲料对海月水母生长性能的影响

    Institute of Scientific and Technical Information of China (English)

    王文龙; 冷向军; 刘晃

    2009-01-01

    研究了5种饲料对海月水母Aurelia aurita(伞体直径为2.50 cm±0.10 cm)生长性能的影响.实验共分5组,每组设3个平行,分别投喂卤虫无节幼体、冰冻卤虫、冷冻干燥卤虫、国产微粒饲料和国外微粒饲料,依照投喂饲料的不同,各组分别记为G_(鲜活)、G_(冰冻)、G_(干燥)、G_(国产)、G_(国外)组,实验共进行30 d.结果表明:饲养30 d后,5组海月水母伞体的平均直径依次为(5.01±0.10)、(4.78±0.20)、(4.62±0.11)、(4.23±0.05)、(4.42±0.06)cm,卤虫无节幼体组生长性能最好,水母伞体直径显著高于其他各组(P0.05),收缩频率平均为0.5~0.66 次/s;冰冻卤虫组的三态氮含量均显著高于其他各组(P<0.05),国内微粒饲料组的三态氮含量次之,也显著高于其他3组(P<0.05).本实验结果表明,卤虫无节幼体是海月水母较理想的饵料,冰冻卤虫和冷冻干燥卤虫次之.国产微粒饲料因为在营养组成、水中稳定性和悬浮性方面较差,饲养海月水母的效果也较差.%Moon jellyfish Amelia aurita with initial umbrella diameter of (2.50 ± 0.10)cm were fed nauplii of Ar-temia, frozen Atemia, freeze dried Atemia, Chinese microdiet and microdiet abroad for one month to evaluate the influence of the diets above on growth performance in the moon jellyfish. The moon jellyfish fed the Chinese micro-diet had survival rate of 95% , whereas the moon jellyfish fed the other diets had survival rate of 100%. The moon jellyfish fed the nauplii of Anemia showed the best growth performance, the umbrella diameter (5.01 cm ±0. 10 cm) being significant higher than the animals fed other diets(P <0.05) .followed by the moon jellyfish fed the fro-zen Atemia (4.78 cm ±0.20 cm in umbrella diameter) or freeze dried Atemia (4.62 cm ±0.11 cm in umbrella diameter). The least growth was observed in the moon jellyfish fed the Chinese microdiet (4.42 cm ±0.06 cm in umbrella diameter). The highest constraction frequency (1/second) was

  15. Rhopalium development in Aurelia aurita ephyrae

    Science.gov (United States)

    Spangenberg, D. B.

    1991-01-01

    Rhopalia of developing ephyrae were examined using the SEM and TEM at 24 h intervals following strobilation induction. Kinocilia are shorter in the ephyra stage than in polyps. A few ephyra-type kinocilia are found in rhopalia as early as 24 h after induction, before a distinct rhopalium is seen. By 72 h, the shorter kinocilia predominate and are almost as numerous as in ephyrae (120 h). Many of the kinocilia are associated with mechanoreceptor cells (MR) found in the rhopalia. These MR cells are compared to those reported for medusae. Although newly released ephyrae lack a touch plate, the MR cells in their rhopalia along with the statocyst and neuromuscular system apparently enable these organisms to detect and respond to gravity.

  16. Influência da ingestão de sardinha nos níveis de ácidos graxos poliinsaturados da série ômega3 no leite materno The influence of sardine consumption on the omega-3 fatty acid content of mature human milk

    Directory of Open Access Journals (Sweden)

    Rose V. Patin

    2006-02-01

    Full Text Available OBJETIVOS: A proposta deste trabalho foi verificar a influência da ingestão de sardinha, alimento rico em ácidos graxos poliinsaturados da série ômega3, na composição do leite materno. MÉTODOS: Estudo prospectivo avaliou 31 nutrizes acompanhadas no Hospital Guilherme Álvaro, as quais receberam 2 kg de sardinha fresca por duas vezes, em intervalos de 15 dias. Nos tempos 0, 15 e 30 dias, realizou-se inquérito alimentar de 24 horas e coleta de leite. Determinaram-se os ácidos graxos do leite materno por cromatografia a gás. Para análise estatística dos resultados, utilizaram-se testes não paramétricos, com nível de significância p OBJECTIVES: The purpose of this study was to investigate what effect the intake of sardines, rich in omega-3 series polyunsaturated fatty acids, has on the composition of breastmilk. METHODS: This was a prospective study of 31 nursing mothers under observation at the Hospital Guilherme Álvaro. Each was given 2 kg of fresh sardines twice with a 15-day interval. Milk was sampled and a 24-hour dietary recall questionnaire was applied on days 0, 15 and 30. Milk was assayed for fatty acid content by gas chromatography. Statistical analysis of the results was performed using nonparametric tests with significance set at p < 0.05. RESULTS: The results demonstrate that the nutritional intake of the nursing mothers was adequate at all three sample points. With regard to the omega-3 series fatty acid content of the breastmilk, it was observed that regular consumption and shorter intervals between intake and milk collection resulted in higher concentrations of docosapentaenoic acid and docosahexaenoic acid at 15 and 30 days into the study. Fatty acids from the omega-3 and omega-6 series exhibited a significant correlation, r² was 0.58 and 0.59 at 15 and 30 days, respectively. CONCLUSION: These results suggest that incorporating fish into the diets of nursing mother during lactation, in the form of 100 g of

  17. Pacific sardine larvae distribution off California

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Marine Sanctuary Program (NMSP) updates and revises the management plans for each of its 13 sanctuaries. This process, which is open to the public,...

  18. Catch rate of juveniles Ethamatosa fimbriata, Sardinella maderensis ...

    African Journals Online (AJOL)

    Administrator

    lift ban on export of fish and fishery products to developed countries ...... California Fish and Game 59: 281-. 292. Wade DL, Jones CM, Robson DS, Pollock KH (1991). Computer. Simulation techniques to assess bias in the roving creel survey.

  19. Assessment of Sardinella and other Small Pelagics in West Africa

    NARCIS (Netherlands)

    Corten, A.A.H.M.

    2004-01-01

    The year 2003 was the sixth year of the cooperation between RIVO and the Mauritanian fisheries research institute IMROP (Institut Mauritanien des Recherches Océanographiques et de Pêche). It was also the second year of the current contract between RIVO and the Dutch Ministry of Agriculture, Nature a

  20. Determinação dos compostos basicos totais de pescado e o seu potencial para avaliação do frescor

    OpenAIRE

    Claudio Chaves Amanajas

    1985-01-01

    Resumo: A presente pesquisa relata as condições analíticas e as aplicações de um novo método químico para avaliação do estado de frescor de pescados. O método foi denominado de Bases Totais (BT) para diferenciá-lo do teste convencional das Bases Voláteis (BV). Nos testes para o desenvolvimento do método, foram usadas as seguintes espécies: sardinha (Sardinella aurita) cavalinha (Scomber scombrus), corvina (Micropogon fournieri) pescada-foguete (Macrodon ancylodon), corimbatã (Prochilodus ret...

  1. Modificación en la composición de ácidos grasos del huevo al incluir aceite de sardina y ácido linoleico conjugado en dietas para gallinas ponedoras Modulation in egg fatty acids composition when laying hens diets are supplemented with sardine oil and conjugated linoleic acid

    Directory of Open Access Journals (Sweden)

    S Carrillo

    2012-01-01

    Full Text Available Actualmente el consumo regular de ácidos grasos omega 3 (C18:3 ALA, C20:5 EPA, C22:6 DHA y de ácido linoleico conjugado (C18:2 CLA es recomendado debido a la importancia que estos compuestos bioactivos tienen en la prevención y control de enfermedades cardiovasculares, diabetes y diferentes tipos de cáncer. El objetivo de este estudio fue determinar el efecto sobre la composición en ácidos grasos del huevo cuando la dieta de las gallinas es suplementada con aceite de sardina y CLA. 240 gallinas Bovans White fueron distribuidas aleatoriamente en cuatro tratamientos con cinco réplicas de 12 aves cada una. Los tratamientos fueron: testigo (T1 con una dieta basal, la misma que T1 más 2,5% de aceite de sardina (T2, la misma que T2 pero adicionando 1% y 2% de CLA (T3 y T4 respectivamente. El ensayo experimental tuvo una duración de cuatro semanas. Al final de este período, 50 huevos de cada tratamiento fueron tomados para realizar la determinación de ácidos grasos por cromatografía de gases. Con respecto al tratamiento testigo, los resultados mostraron un incremento en la concentración total de ácidos grasos omega 3 (1,6% vs 6,0% del total de ácidos grasos y de CLA (0,7% vs 3-5% del total de ácidos grasos en el huevo de los tratamientos T3, T4 y T5 en conjunto, y una relación n6:n3 de 11:1 vs 1.3:1 (P The regular intake of omega 3 fatty acids (C18:3 ALA, C20:5 EPA, C22:6 DHA and conjugated linoleic acid (C18:2 CLA is currently recommended due to their importance in the prevention and control of cardiovascular diseases, diabetes and several types of cancer. The aim of this study was to determine the effect of supplementing laying hens diets with sardine oil and CLA on egg fatty acid composition. 240 Bovans White hens were randomly distributed into four treatments with 5 replicates of 12 hens each one. The treatments consisted in a control diet (T1, T1 plus 2.5% of sardine oil (T2, and T2 padded with 1% and 2% of CLA (T3 and T4

  2. Diel and vertical movements of preflexion fish larvae assemblage associated with Posidonia oceanica beds

    Directory of Open Access Journals (Sweden)

    Yoana del Pilar Ruso

    2006-09-01

    Full Text Available Habitat complexity is a strong determinant of fish assemblage structure. Enhanced food resources and protection from predators are generally considered the main benefits of vegetated areas. We investigated the diel and vertical movements of preflexion fish larvae within and above the leaf canopy of Posidonia oceanica to determine if it acts as an important habitat for larval stage fish. Samples were taken with moored plankton nets during the night and day at two depths (13 and 15 m over five consecutive days in July 2000 in the Marine Protected Area off Tabarca Island (Spain, SW Mediterranean Sea. In total, 353 fish larvae were captured. The most abundant taxa were Sardinella aurita, Sparidae, Engraulis encrasicolus and Gobiidae. Sparidae were more abundant within the leaf canopy during the day but increased in waters 2 m above the canopy at night. In contrast, Sardinella aurita were present throughout the entire water column during both the day and night. We conclude that the P. oceanica leaf canopy is important as a transitional shelter for the early stage larvae of some demersal fish species.

  3. Clasificación acústica de anchoveta (Engraulis ringens y sardina común (Strangomera bentincki mediante máquinas de vectores soporte en la zona centro-sur de Chile: efecto de la calibración de los parámetros en la matriz de confusión Acoustic classification of anchovy (Engraulis ringens and sardine (Strangomera bentincki using support vector machines in central-southern Chile: effect of parameter calibration on the confusion matrix

    Directory of Open Access Journals (Sweden)

    Hugo Robotham

    2012-03-01

    Full Text Available Se clasificó la anchoveta (Engraulis ringens y sardina común (Strangomera bentincki detectadas mediante equipos acústicos en la zona centro-sur de Chile, mediante el método de Máquinas de Vectores Soporte (SVM. Para esto se utilizaron descriptores de cardúmenes extraídos desde ecogramas, que fueron clasificados como morfológicos, batimétricos, energéticos y posicional espacial. Para lograr clasificaciones precisas mediante la utilización de esta metodología, fue necesario optimizar parámetros correspondientes al Kernel-Gaussiano, γ y de penalización del modelo C, mediante el análisis del efecto de la calibración sobre las matrices de confusión resultantes de la clasificación de las especies analizadas. El método SVM ajustó correctamente el 95,3% de los cardúmenes de anchoveta y sardina común. Los parámetros óptimos del Kernel-Gaussiano γ y de penalización C obtenidos mediante la metodología propuesta fueron γ = 450 y C = 0,95, respectivamente. Los parámetros mencionados incidieron de manera importante en la matriz de confusión y los porcentajes de clasificación final, por lo que se sugiere establecer, en aplicaciones futuras de este método, un protocolo experimental de calibración. La sardina común fue la especie con menor error de clasificación en el conjunto de las matrices de confusión. El descriptor correspondiente a profundidad del fondo fue el más sensible al SVM, la segunda variable en importancia es el descriptor distancia a la costa.The support vector machines (SVM method was used to classify the anchovy (Engraulis ringens and common sardine (Strangomera bentincki species detected in south-central Chile by means of acoustic equipment. For this, descriptors of fish schools (morphology, bathymetry, energy, spatial position extracted from ecograms were used. In order to obtain precise classifications using this methodology, it was necessary to optimize the parameters Gaussian-Kernel γ and penalty term

  4. Traditionally, sardine Sardinops sagax has been the backbone of ...

    African Journals Online (AJOL)

    denise

    Grant Program, San Diego, California, USA, in cooperation with Centro de Investigaciones ... maturity of Baltic cod (Gadus morhua) during a period of large variations in stock .... Trudy– AtlantNIRO 73: 77–85 (in Russian). ROCHET, M. J. 1998 ...

  5. Anchovy Engraulis capensis, sardine Sardinops sagax and round ...

    African Journals Online (AJOL)

    spamer

    all years in the recruitment time-series from 1985 to. 1994. ... Forecasts of anchovy recruitment from multiple cruises may produce a different result from the forecast ... abundance, phyto- and zooplankton biomass, copepod ...... estuarine lake.

  6. The southern African sardine Sardinops sagax (also known as ...

    African Journals Online (AJOL)

    denise

    mental factors that affect stock dynamics primarily through recruitment, and .... school-groups contributed an increasing proportion ..... of a recovery in the stock in the early 1990s were not ..... must strive to promote a healthy spawning stock,.

  7. The southern African sardine Sardinops sagax supports major purse ...

    African Journals Online (AJOL)

    spamer

    Echo-Integrator post-processing system (Foote et al. 1991). ... noise-reduction filters at weak, and with frequency ..... approach based on acoustic image simulation. Aquat. ... GERLOTTO, F., SORIA, M. and P. FRÉON 1999 — From 2D to.

  8. Composição em aminoácidos de silagens químicas, biológicas e enzimáticas preparadas com resíduos de sardinha Determination of amino-acid composition of silages prepared from sardine residues

    Directory of Open Access Journals (Sweden)

    Doris Floridalma MORALES-ULLOA

    1997-12-01

    Full Text Available Determinou-se a composição em aminoácidos de silagens químicas, biológicas e enzimáticas elaboradas com resíduos de sardinha. Entre os aminoácidos essenciais a leucina apresentou valores mais altos para todas as silagens, a saber, em g/100g de proteína, 8,31 (química; 8,33 (protease 1 semana; 8,42 (pepsina, e 8,06 (inóculo L. plantarum + melaço 2 semanas, seguida pela lisina 6,46; 6,50 6,45, e 9,01; a fenilalanina com 5,32; 5,35 e 5,25 e 5,18. Destaque especial para o aumento na concentração de valina no decorrer do processo de ensilagem passando de 4,80 g/100g de proteína na matéria-prima para 7,67 na silagem química (3 semanas; 6,26 na silagem com meio inóculo de L.plantarum + melaço (48 horas; 6,27 na silagem protease (1 semana e 6,02 na silagem pepsina (2 semanas. A maior concentração de aminoácidos encontrados foi para o ácido glutâmico, que apresentou teor inicial de 15,20g/100g de proteína e posteriormente 14,02 na silagem química após 1 semana; 14,89 na silagem enzimática com protease (1 semana e 17,09 na silagem biológica com meio inóculo L.plantarum + melaço após 48 horas.The composition and amino-acid concentration of chemical, biological and enzymatic silages prepared from sardine residues, were determined. The essential amino-acid, leucine showed the highest values in all silages (8.31; 8,33 8.42; and 8.06 g/100g protein, followed by lysine (6.46, 6.50, 6.45 and 9,.01 g/100 protein, for chemical silage, protease silage after one week pepsin silage in the L. plantarum after 2 weeks respectively. Phenylalanine showed a value of 5.32g/100g protein in the chemical silages after one week, 5.35g/100g in the protease silage after one week, 5.25g/100g in the pepsin silage after two weeks and 5.18g/100g in the silage inoculated with L. plantarum plus its medium, also after two weeks. The increase in the valine during the silage processing deserves special mention, increasing from 4.80/100g protein in the raw

  9. Digenean species diversity in teleost fishes from the Gulf of Gabes, Tunisia (Western Mediterranean

    Directory of Open Access Journals (Sweden)

    Derbel H.

    2012-05-01

    Full Text Available This study is the first attempt to survey the diversity of fish digeneans in the Gulf of Gabes (southern coast of Tunisia. A total of 779 fishes belonging to 32 species were sampled. 53 species of Digenea belonging to 15 families were recorded. Among these species, 24 are reported for the first time from the coast of Tunisia. We report one new host record, Lecithochirium sp. from Sardinella aurita. The Hemiuridae is the dominant family. A host-parasite list is presented with the information on the prevalence, abundance and mean intensity of each species collected. The diversity of Digenea is compared with other localities in the Mediterranean Sea and the northern east of Tunisia. The Gulf of Gabes shows the lowest diversity linked to the anthropogenic activities and impact of exotic species. The use of Digenea as indicators of the state of the ecosystem is discussed.

  10. Interannual variation of larval fish assemblages in the Gulf of Cádiz (SW Iberian Peninsula in relation to summer oceanographic conditions

    Directory of Open Access Journals (Sweden)

    Paulo Mafalda Júnior

    2006-03-01

    Full Text Available Two ichthyoplankton surveys were conducted during July 1994 and July 1995 in the Gulf of Cádiz with the aim of describing composition, abundance, distribution patterns and interannual variations of larval fish assemblages. Interannual differences were found in this study. In 1994, higher salinities were observed at external sites, though in 1995, higher values were observed at intermediate sites. The upper water column was warmer in 1994 and had less fish larvae density. During 1994, Sardinella aurita and Engraulis encrasicolus were abundant but spatial location was opposite. In 1995, abundance of both species was very different, but with similar spatial pattern. Cluster analysis revealed well-defined groups of stations and assemblages of larvae, primarily related to bathymetry. The "inshore assemblage" occupied the shallow coast area; its characteristics species being closely related to the estuarine system, mainly comprising Engraulis encrasicolus and Gobiidae. The "shelf assemblage" occupied the continental shelf and its characteristics species consisted of larvae whose adults inhabited the shelf province and spawn in the same zone, like Sardinella aurita and Trachurus spp. Interannual variations in composition and extension of the subgroups could be attributed to the main circulation patterns, continental water discharge and spawning strategies of fishes.Duas amostragens de ictioplâncton foram realizadas durante Julho de 1994 e Julho de 1995, no Golfo de Cádiz, com o objetivo de descrever a composição, abundância, padrões de distribuição e variações interanuais das associações de larvas de peixes. Diferenças interanuais foram encontradas neste estudo. Em 1994 salinidades mais elevadas foram observadas nas estações da plataforma externa, enquanto que, em 1995 os valores mais elevados foram encontrados nas estações da plataforma interna. A coluna de água foi mais quente em 1994 e apresentou menor densidade de larvas de

  11. Application of satellite imagery to analyse the distribution and recruitment of sardinella - Annual Report 2002

    NARCIS (Netherlands)

    Zeeberg, J.J.

    2003-01-01

    Remote sensing studies at the Netherlands Institute for Fisheries Research (RIVO) aim to stimulate the use of satellite images on board of Dutch freezer-trawlers in the Mauritanian Exclusive Economic Zone (MEEZ). During four research missions (5-12 July 2002, 23-30 August 2002, 13-20 October 2002, a

  12. Genetic relationships among three species of the genus @iSardinella@@ (Clupeidae)

    Digital Repository Service at National Institute of Oceanography (India)

    Menezes, M.R.

    no alleles. Nei's genetic distance values ranged from 0.31 + or - 0.15 to 1.68 + or - 0.51. Four loci were polymorphic in @iS. albella@@, and three in @iS. fimbriata@@ at P=0.95 level. No locus was polymorphic by the 95% criterion in @iS. longiceps@@. Average...

  13. Stomach contents of cetaceans incidentally caught along Mangalore and Chennai coasts of India

    Science.gov (United States)

    Krishnan, Anoop A.; Yousuf, K. S.; Kumaran, P. L.; Harish, N.; Anoop, B.; Afsal, V. V.; Rajagopalan, M.; Vivekanandan, E.; Krishnakumar, P. K.; Jayasankar, P.

    2008-03-01

    The stomachs of 32 individuals of seven cetacean species incidentally caught in gill net and purseseine fisheries along Mangalore and Chennai coasts (India) between 2004 and 2006 were examined. The whole stomach (fore-gut, mid-gut and hind-gut) was examined in all cases. Prey remains (666 prey items comprising six species of teleosts, one crustacean and one squid species) were found in the stomachs of eight individuals (the remaining 24 stomachs were found to be empty). All cetaceans were found to feed mostly on teleosts with wide range of trophic levels. Based on an index that included frequency of occurrence, percentage by number and by weight, the oil sardine Sardinella longiceps was the main prey in the sample. Cetaceans appear to favour both pelagic as well as demersal prey, possibly indicating surface and benthic feeding habits.

  14. Levels of polonium-210 in highly consumed sea foods from a fish market of the city of Niteroi, RJ-Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Marsico, Eliane T.; Sao Clemente, Sergio C. de [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil). Faculdade de Veterinaria. Dept. de Tecnologia de Alimentos; Kelecom, Alphonse; Gouvea, Rita de Cassia S. [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil). Dept. de Biologia Geral. Lab. de Radiobiologia e Radiometria Pedro Lopes dos Santos - LARARA

    2007-07-01

    Polonium-210 ({sup 210}Po), a short-lived member from the uranium series, is broadly distributed in Nature being among all alpha-emitters the major contributor to the internal dose in man. Studies of diets have shown that marine foods are important sources of this radionuclide. The levels of {sup 210}Po have been determined in three highly consumed marine species, the fishes Sardinella brasiliensis (sardine) and Thunnus atlanticus (tuna), and the shrimp Litopenaeus brasiliensis, purchased from the Niteroi (RJ, Brazil) fish market, and caught along the coast of the state of Rio de Janeiro. Doses of {sup 210}Po were determined for the entire organisms and for some tissues and organs, such as eyes, heart, gills, muscle, stomach liver, intestine and pyloric caecal (fishes) or eyes, head content, exoskeleton, muscle, hepatopancreas and pleopods. {sup 210}Po is not uniformly distributed within these species, the highest levels being observed for sardine in the intestine (1634.6 mBq g-1), tuna in pyloric caecal (4656.1 mBq g-1) and shrimp in the hepatopancreas (1460.5 mBq g-1). The {sup 210}Po activities in sardine, tuna and shrimp, calculated on a mean whole-organism basis, were 64.6, 34.5 and 39.5 Bq kg-1 (dry weight) respectively, with corresponding concentration factors of 7.2 x104, 3.8 x104 and 4.4 x104. Considering body distribution, almost 60% of the total activity is concentrated in the pyloric caecal of both fishes and in the hepatopancreas of the shrimp. In turn, the edible parts concentrate much less activity. (author)

  15. NMR study on a novel mucin from jellyfish in natural abundance, Qniumucin from Aurelia aurita.

    Science.gov (United States)

    Uzawa, Jun; Urai, Makoto; Baba, Takayuki; Seki, Hiroko; Taniguchi, Kayoko; Ushida, Kiminori

    2009-05-22

    A novel mucin (qniumucin), which we recently discovered in jellyfish, was investigated by several NMR techniques. Almost all the peaks in the (13)C and proton NMR spectra were satisfactorily assigned to the amino acids in the main chain and to the bridging GalNAc, the major sugar in the saccharide branches. The amino acid sequence in the tandem repeat part (-VVETTAAP-) was reconfirmed by the cross-peaks between alpha protons and carbonyl carbons in the HMBC spectrum. A connectivity analysis around the O-glycoside bond (GalNAc-Thr) was also performed, and detailed information on the local configuration was obtained by the DPFGSE-NOE-HSD technique. The strategy and the results described in this paper can be extended to the structural analysis of general O-glycan chains, which are more complex than the present mucin. NMR analyses reveal the simple structure of qniumucin extracted by the present protocol, and the homogeneity and purity of qniumucin are probably the result of it being extracted from jellyfish, a primitive animal.

  16. Environmental conditions influencing the larval fish assemblage during summer in the Gulf of Gabes (Tunisia: South central Mediterranean

    Directory of Open Access Journals (Sweden)

    W. K. KOCHED

    2015-11-01

    Full Text Available An oceanographic survey was carried out during the summer of 2009, from June 25 to July 3 in the Gulf of Gabes. The study area is characterized by a wide continental shelf.A plankton sampling scheme was carried out to study the structure of the larval fish assemblages that characterizes the surface mixed layer, and to assess the influence of the environmental parameters on their spatial distribution.A total of 36 taxa were identified, 3 classified to the level of genus, 5 to family and 28 to species. The majority of identified fish larvae belonged to demersal species, mainly picarel Spicara smaris (23.76% of the total larval fish abundance, followed by red mullet Mullus barbatus (14.92% and several species of the Sparidae family (9.15%. Small pelagic species as Sardinella Sardinella aurita and European anchovy Engraulis encrasicolus were also abundant (27.75 % and dominated the fish larvae community in offshore stations. Five large pelagic species were found among the larvae, including bullet tuna Auxis rochei, Atlantic black skipjack Euthynnus alletteratus, albacore Thunnus alalunga, swordfish Xiphias gladius and the highly migratory Atlantic bluefin tuna Thunnus thynnus. All these apex predator species appear in 38.5% of the sampling stations and contribute about 7.9% of the total larval catches. The larval fish assemblage of the Gulf of Gabes is predominantly composed of neritic species in correspondence with the shallow depths of the study area. Canonical Correspondence Analysis (CCA showed that larval distributions were mainly conditioned by depth. However, other factors, like hydrographic processes, play also a very important role in shaping the spatial pattern of larval fish distribution.

  17. Mercury and selenium in seston, marine plankton and fish (Sardinella brasiliensis) as a tool for understanding a tropical food web.

    Science.gov (United States)

    Seixas, Tércia Guedes; Moreira, Isabel; Kehrig, Helena Amaral

    2015-12-15

    Mercury (Hg) and selenium (Se) concentrations were evaluated in a planktivorous fish and four size classes of organisms (FSCO), collected at an oligotrophic bay in the Southeastern Brazilian coast. No significant spatial differences between Hg and Se were found in the FSCO within the five sampling points in the bay. Hg and Se concentrations increased with successive increases in the size class of the analyzed plankton, i.e. approximately 3-and 2-fold, respectively, from microplankton to macroplankton. Hg and Se biomagnified throughout the planktonic food web. The smallest size class of organism, seston, composed of both biotic and abiotic portions, and fish showed the highest Hg concentrations. This indicates that Hg is not biomagnifying in the base of the bay food web. Selenium concentrations in fish were approximately 5.9 times higher than those in seston. Hg and Se concentrations in fish were approximately 3.5 and 14.6 times higher than those found in the plankton, respectively.

  18. Artificial neural networks to forecast biomass of Pacific sardine and its environment

    DEFF Research Database (Denmark)

    Cisneros Mata, M.A.; Brey, T.; Jarre, Astrid

    1996-01-01

    We tested the forecasting performance of artificial neural networks (ANNs) using several time series of environmental and biotic data pertaining to the California Current (CC) neritic ecosystem. ANNs performed well predicting CC monthly 10-m depth temperature up to nine years in advance, using te...

  19. Human exposure to mercury, lead and cadmium through consumption of canned mackerel, tuna, pilchard and sardine.

    Science.gov (United States)

    Okyere, H; Voegborlo, R B; Agorku, S E

    2015-07-15

    Total mercury (Hg), cadmium (Cd) and lead (Pb) concentrations were determined in canned fish on the Ghanaian market. Total mercury was determined using an automatic mercury analyzer while cadmium and lead levels were determined by flame atomic absorption spectrophotometry. The metal contents in the samples, expressed in μg g(-1) (wet weight), varied from canned fish from the Ghanaian market have concentrations well below the permissible FAO/WHO for these toxic metals. Thus considering the Provisional Tolerable Weekly Intake (PTWI) of Hg, Pb and Cd the levels obtained in this study are unlikely to constitute a significant exposure to the public through consumption of moderate amounts.

  20. Artificial neural networks to forecast biomass of Pacific sardine and its environment

    DEFF Research Database (Denmark)

    Cisneros Mata, M.A.; Brey, T.; Jarre, Astrid

    1996-01-01

    We tested the forecasting performance of artificial neural networks (ANNs) using several time series of environmental and biotic data pertaining to the California Current (CC) neritic ecosystem. ANNs performed well predicting CC monthly 10-m depth temperature up to nine years in advance, using te...

  1. Oil sardine and Indian mackerel: Their fishery, problems and coastal oceanography

    Digital Repository Service at National Institute of Oceanography (India)

    Madhupratap, M.; Shetye, S.R.; Nair, K.N.V.; Nair, S.R.S.

    -leading to the productivity are examined to find their relationship to the fishery. However, this study attempts to point out the drawbacks in our understanding and proposes research problems that could be pursued...

  2. A peptidomic approach for the identification of antioxidant and ACE-inhibitory peptides in sardinelle protein hydrolysates fermented by Bacillus subtilis A26 and Bacillus amyloliquefaciens An6.

    Science.gov (United States)

    Jemil, Ines; Mora, Leticia; Nasri, Rim; Abdelhedi, Ola; Aristoy, Maria-Concepción; Hajji, Mohamed; Nasri, Moncef; Toldrá, Fidel

    2016-11-01

    Antioxidant and angiotensin I-converting enzyme (ACE)-inhibitory activities of sardinelle (Sardinella aurita) protein hydrolysates (SPHs) obtained by fermentation with Bacillus subtilis A26 (SPH-A26) and Bacillus amyloliquefaciens An6 (SPH-An6) were investigated. Both hydrolysates showed dose-dependent antioxidant activities evaluated using various in vitro antioxidant assays. Further, they were found to exhibit ACE-inhibitory activity. Peptides from SPH-A26 and SPH-An6 were analyzed by nESI-LC-MS/MS and approximately 800 peptides were identified. Identified peptides derived mainly from myosin (43% and 31% in SPH-An6 and SPH-A26, respectively). Several peptides identified in both hydrolysates were found to share sequences with previously identified antioxidant and ACE-inhibitory peptides based on Biopep database. Some of these peptides were selected for synthesis and their biological activities were evaluated. Among the synthesized peptides, NVPVYEGY and ITALAPSTM were found to be the most effective ACE-inhibitors with IC50 values of 0.21 and 0.23mM, respectively. On the other hand, NVPVYEGY, which exhibited the highest ACE-inhibitory activity, showed the highest reducing power and peroxyl radical scavenging activities, followed by SLEAQAEKY and GTEDELDKY. The results of this study suggest that fermented sardinelle protein hydrolysates are a good source of natural antioxidant peptides and could have the potential to act as hypotensive nutraceutical ingredients. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. Hypolipidemic, antiobesity and cardioprotective effects of sardinelle meat flour and its hydrolysates in high-fat and fructose diet fed Wistar rats.

    Science.gov (United States)

    Jemil, Ines; Abdelhedi, Ola; Nasri, Rim; Mora, Leticia; Marrekchi, Rim; Jamoussi, Kamel; ElFeki, Abdelfattah; Hajji, Mohamed; Toldrá, Fidel; Nasri, Moncef

    2017-05-01

    The present study aims to evaluate the antiobesity, hypolipidemic and cardioprotective effects of fermented sardinelle (Sardinella aurita) protein hydrolysates (FSPHs) produced with two proteolytic bacteria, Bacillus subtilis A26 (FSPH-A26) and Bacillus amyloliquefaciens An6 (FSPH-An6). Wistar rats were fed during 10weeks a standard laboratory diet, a high caloric diet (HCD) and a HCD coupled with the oral administration of sardinelle meat flour (SMF) or FSPHs. HCD caused hyperlipidemia and increased body weight (BW). The daily oral administration of FSPHs or SMF reduced the total cholesterol (TC), triglycerides (TG) and low-density lipoprotein cholesterol (LDL-c) serum levels, and increased the level of high-density lipoprotein cholesterol (HDL-c). Nevertheless, FSPHs were found to be more efficient than SMF. FSPHs also lowered hepatic TC and TG content and decreased the pancreatic lipase activity. Further, the administration of FSPHs or SMF decreased the BW gain, the food intake and the relative epididymal adipose tissue weight. FSPHs exhibited a potent cardioprotective effect against heart attack, which was demonstrated by returning atherogenic indexes to their normal levels and the conservation of standard histological structure of the heart and aorta. The overall results indicate that FSPHs contained bioactive peptides which significantly attenuated hyperlipidemia, and might reduce the risk of cardiovascular disease (CVD) in rats fed HCD. Copyright © 2016 Elsevier Inc. All rights reserved.

  4. Metal contents in common edible ifsh species and evaluation of potential health risks to consumers

    Institute of Scientific and Technical Information of China (English)

    Naglaa Farag Soliman; Samir Mahmoud Nasr

    2015-01-01

    Objective:To conduct a health risk assessment of some heavy metals attributed to consumption of common edible fish species available for consumers. Methods: Concentrations of Cd, Cr, Cu, Fe, Mn, Pb and Zn were determined in muscles, gills, livers, bones and skins of six common edible fish species, namelyOreochromis niloticus, Mugil cephalus, Sardinella aurita, Mullus barbatus, Boops boops, Pagrus pagrus. Concentrations of heavy metals were determined by atomic absorption spectrophotometer and expressed asµg/g of wet tissue. Results:Results showed that iron and zinc were the most abundant among all fish tissues under investigation. The data obtained in the present work were compared well with the counterpart data reported internationally. The estimated values of all metals in muscles of fish in this study were below the permissible limits. Moreover, the potential health risks of metals to human via consumption of seafood were assessed by estimating daily intake and target heath quotient. Generally, risk values for the measured metals do not pose unacceptable risks at mean ingestion rate for muscles. Conclusions: It can be concluded that the investigated metals in edible parts of the examined species have no health problems for consumers.

  5. Proto-cooperation: group hunting sailfish improve hunting success by alternating attacks on grouping prey.

    Science.gov (United States)

    Herbert-Read, James E; Romanczuk, Pawel; Krause, Stefan; Strömbom, Daniel; Couillaud, Pierre; Domenici, Paolo; Kurvers, Ralf H J M; Marras, Stefano; Steffensen, John F; Wilson, Alexander D M; Krause, Jens

    2016-11-16

    We present evidence of a novel form of group hunting. Individual sailfish (Istiophorus platypterus) alternate attacks with other group members on their schooling prey (Sardinella aurita). While only 24% of attacks result in prey capture, multiple prey are injured in 95% of attacks, resulting in an increase of injured fish in the school with the number of attacks. How quickly prey are captured is positively correlated with the level of injury of the school, suggesting that hunters can benefit from other conspecifics' attacks on the prey. To explore this, we built a mathematical model capturing the dynamics of the hunt. We show that group hunting provides major efficiency gains (prey caught per unit time) for individuals in groups of up to 70 members. We also demonstrate that a free riding strategy, where some individuals wait until the prey are sufficiently injured before attacking, is only beneficial if the cost of attacking is high, and only then when waiting times are short. Our findings provide evidence that cooperative benefits can be realized through the facilitative effects of individuals' hunting actions without spatial coordination of attacks. Such 'proto-cooperation' may be the pre-cursor to more complex group-hunting strategies.

  6. Preliminary results of the in vivo and in vitro characterization of a tentacle venom fraction from the jellyfish Aurelia aurita.

    Science.gov (United States)

    Ponce, Dalia; López-Vera, Estuardo; Aguilar, Manuel B; Sánchez-Rodríguez, Judith

    2013-12-06

    The neurotoxic effects produced by a tentacle venom extract and a fraction were analyzed and correlated by in vivo and in vitro approaches. The tentacle venom extract exhibited a wide range of protein components (from 24 to >225 kDa) and produced tetanic reactions, flaccid paralysis, and death when injected into crabs. Two chromatography fractions also produced uncontrolled appendix movements and leg stretching. Further electrophysiological characterization demonstrated that one of these fractions potently inhibited ACh-elicited currents mediated by both vertebrate fetal and adult muscle nicotinic acetylcholine receptors (nAChR) subtypes. Receptor inhibition was concentration-dependent and completely reversible. The calculated IC(50) values were 1.77 μg/μL for fetal and 2.28 μg/μL for adult muscle nAChRs. The bioactive fraction was composed of a major protein component at ~90 kDa and lacked phospholipase A activity. This work represents the first insight into the interaction of jellyfish venom components and muscle nicotinic receptors.

  7. Settlement of planulae of the Moon jellyfish Aurelia aurita onto hydrophilic polycarbonate plates modified by atmospheric plasma treatment.

    Science.gov (United States)

    Tomaru, Akiko; Sasaki, Ryota; Miyahara, Hidekazu; Okino, Akitoshi; Ogawa, Nobuhiro; Hamasaki, Koji

    2014-01-01

    It has been reported that planula larvae of some jellyfish prefer artificial substrates for settlement. This research focused on the relationship between the settlement of planulae and the wettability of artificial substrate surfaces. We used atmospheric plasmas to change the wettability of the surfaces of polycarbonate (PC) plates because plasma treatment has no chemical side effects. The treatment made the surfaces hydrophilic, as evidenced by the decrease of contact angle from 85° to 35°. X-ray photoelectron spectroscopy revealed that the change of wettability of the PC plates could be attributed to N2, which was probably ionized in the air above the plates. Scanning electron microscopy revealed no difference in the surface morphology of the plates before and after plasma treatment. Results of bioassays using treated PC plates showed that planulae tended to preferentially settle on hydrophobic surfaces.

  8. Preliminary Results of the in Vivo and in Vitro Characterization of a Tentacle Venom Fraction from the Jellyfish Aurelia aurita

    Science.gov (United States)

    Ponce, Dalia; López-Vera, Estuardo; Aguilar, Manuel B.; Sánchez-Rodríguez, Judith

    2013-01-01

    The neurotoxic effects produced by a tentacle venom extract and a fraction were analyzed and correlated by in vivo and in vitro approaches. The tentacle venom extract exhibited a wide range of protein components (from 24 to >225 kDa) and produced tetanic reactions, flaccid paralysis, and death when injected into crabs. Two chromatography fractions also produced uncontrolled appendix movements and leg stretching. Further electrophysiological characterization demonstrated that one of these fractions potently inhibited ACh-elicited currents mediated by both vertebrate fetal and adult muscle nicotinic acetylcholine receptors (nAChR) subtypes. Receptor inhibition was concentration-dependent and completely reversible. The calculated IC50 values were 1.77 μg/μL for fetal and 2.28 μg/μL for adult muscle nAChRs. The bioactive fraction was composed of a major protein component at ~90 kDa and lacked phospholipase A activity. This work represents the first insight into the interaction of jellyfish venom components and muscle nicotinic receptors. PMID:24322597

  9. Preliminary Results of the in Vivo and in Vitro Characterization of a Tentacle Venom Fraction from the Jellyfish Aurelia aurita

    Directory of Open Access Journals (Sweden)

    Dalia Ponce

    2013-12-01

    Full Text Available The neurotoxic effects produced by a tentacle venom extract and a fraction were analyzed and correlated by in vivo and in vitro approaches. The tentacle venom extract exhibited a wide range of protein components (from 24 to >225 kDa and produced tetanic reactions, flaccid paralysis, and death when injected into crabs. Two chromatography fractions also produced uncontrolled appendix movements and leg stretching. Further electrophysiological characterization demonstrated that one of these fractions potently inhibited ACh-elicited currents mediated by both vertebrate fetal and adult muscle nicotinic acetylcholine receptors (nAChR subtypes. Receptor inhibition was concentration-dependent and completely reversible. The calculated IC50 values were 1.77 μg/μL for fetal and 2.28 μg/μL for adult muscle nAChRs. The bioactive fraction was composed of a major protein component at ~90 kDa and lacked phospholipase A activity. This work represents the first insight into the interaction of jellyfish venom components and muscle nicotinic receptors.

  10. Settlement of planulae of the Moon jellyfish Aurelia aurita onto hydrophilic polycarbonate plates modified by atmospheric plasma treatment.

    Directory of Open Access Journals (Sweden)

    Akiko Tomaru

    Full Text Available It has been reported that planula larvae of some jellyfish prefer artificial substrates for settlement. This research focused on the relationship between the settlement of planulae and the wettability of artificial substrate surfaces. We used atmospheric plasmas to change the wettability of the surfaces of polycarbonate (PC plates because plasma treatment has no chemical side effects. The treatment made the surfaces hydrophilic, as evidenced by the decrease of contact angle from 85° to 35°. X-ray photoelectron spectroscopy revealed that the change of wettability of the PC plates could be attributed to N2, which was probably ionized in the air above the plates. Scanning electron microscopy revealed no difference in the surface morphology of the plates before and after plasma treatment. Results of bioassays using treated PC plates showed that planulae tended to preferentially settle on hydrophobic surfaces.

  11. Scale-up of an alkaline protease from Bacillus pumilus MTCC 7514 utilizing fish meal as a sole source of nutrients.

    Science.gov (United States)

    Gupta, Rishikesh Kumar; Prasad, Dinesh; Sathesh, Jaykumar; Naidu, Ramachandra Boopathy; Kamini, Numbi Ramudu; Palanivel, Saravanan; Gowthaman, Marichetti Kuppuswami

    2012-09-01

    Fish meal grades SL1 and SL2 from Sardine (Sardinella longiceps) and NJ from Pink Perch (Nemipterus japonicas) were evaluated as a sole source of carbon and nitrogen in the medium for alkaline protease production by Bacillus pumilus MTCC 7514. The analysis of the fish meal suggests that the carbon and nitrogen contents in fish meal are sufficient to justify its choice as replacement for other nutrients. Protease production increased significantly (4,914 U/ml) in medium containing only fish meal, compared with the basal medium (2,646 U/ml). However, the elimination of inorganic salts from media reduced the protease productivity. In addition, all the three grades of fish meal yielded almost the same amounts of protease when employed as the sole source of carbon and nitrogen. Nevertheless, the best results were observed in fish meal SL1 medium. Furthermore, protease production was enhanced to 6,966 U/ml and 7,047 U/ml on scaling up from flask (4,914 U/ml) to 3.7 and 20 L fermenters, respectively, using fish meal (10 g/l). Similarly, the corresponding improvement in productivities over flask (102.38 U/ml/h) was 193.5 and 195.75 U/ml/h in 3.7 and 20 L fermenters, respectively. The crude protease was found to have dehairing ability in leather processing, which is bound to have great environmental benefits.

  12. Collagen Extract from Marine Finfish Scales as a Potential Mosquito Larvicide.

    Science.gov (United States)

    Muthumari, K; Anand, M; Maruthupandy, M

    2016-12-01

    Collagen is a peptide being utilized in medical, health care, nutrient and decorative industry. Marine fish scales are one of the good sources of collagen, which is extracted using the advanced enzymatic digestion method. Scales of Sardinella longiceps (Oil Sardine) have a high proportion of collagen. This product is well absorbed with broad adaptive values that encourage the inclusion of nutriments. In this paper, we have performed the isolation and characterization of collagen from S. longiceps fish scales. The unnecessary proteins on the surface of fish scales was removed by demineralization process. The fish scale collagen was extracted in two different methods: acid (acetic acid) and enzymatic (pepsin) technique. The molecular mass of the extracted collagen was determined using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The absorption spectra of the extracted collagen was measured to estimate its amino acid (tyrosine) content. Fourier transform infrared (FTIR) spectrum showed the existence of bands corresponding to the collagen extracted from S. longiceps fish scale and the crystallinity of extracted collagen was obtained using X-ray diffraction (XRD) analysis. The morphological micrograph was also analyzed by scanning electron microscope (SEM). The anti-larval effect of the collagen extract was determined using mosquito larvae of Aedes aegypti (Ae. aegypti) and the activity was statistically significant.

  13. 北部湾鲔(Euthynnus affinis)的摄食习性及其随生长发育的变化%Feeding Habits and Ontogenetic Diet Shifts of Mackerel Tuna (Euthynnus affinis) in the Beibu Gulf, South China Sea

    Institute of Scientific and Technical Information of China (English)

    宿鑫; 李永东; 何雄波; 卢伙胜; 颜云榕

    2015-01-01

    To study the mackerel tuna (Euthynnus affinis) feeding habits and its ontogenetic diet shifts, 435 mackerel tuna were collected for an annual research from September 2008 to September 2009 in the main fishing ports such as Beihai, Basuo, Sanya and Jianghong of the Beibu Gulf, South China Sea. Results showed that the mackerel tuna of the Beibu Gulf was omnivorous and it predated not only the small pelagic fish but also the benthic fish, crustaceans and cephalopods. Pisces were dominant in the diets with the percentage of Index of Relative Importance (IRI%) of 98.57%. Among these, 21 species were identified. As indicated with IRI%, Bregmaceros rarisquamosus (45.50%), Stolephorus zollingeri (11.81%), Theyssa setirostris (5.89%), and Sardinella aurita (5.87%) were principal in the food cons-ituent. The main species changed monthly and it was Sardinella aurita in January; Bregmaceros rarisquamosus in February, March, November, December; Unidentified Pisces from April to July;Sardinella aurita in August; Leignathus riviulatus in September; Stolephorus zollingeri in October. The prey weight and numbers increased when body size of mackerel tuna was less than 340 mm (SL<340 mm), but there were decreased when body size of mackerel tuna was from 341 to 520 (341-520 mm). The spawning periods of the mackerel tuna were spring and summer, mainly from April to August. There were two spawn peaks, June and August, which were recognized by the female gonad somatic index (GSI) that maintained strong feeding intensity during the reproduction periods. Moreover, the peak periods were consistent with the water temperature of pelagic layers provided by France CATSAT fisheries remote sensing system.%基于2008年9月-2009年9月在北部湾逐月采集的435尾鲔(Euthynnus affinis)生物学数据,对鲔生长、摄食习性及其随生长发育的变化进行了分析。结果显示,北部湾鲔属于广食性鱼类,主要摄食中上层小型鱼类,另外还包括底栖鱼类、

  14. Variations of fish community structure in the waters adjacent to Fugong in Jiulongjiang Estuary%福建九龙江口浮宫附近水域鱼类的群落结构变动

    Institute of Scientific and Technical Information of China (English)

    张会军; 黄良敏; 李军; 王家樵; 张雅芝; 吝涛; 常国芳

    2013-01-01

    2011年1 ~12月,于每月大潮时采用定置张网在九龙江口浮宫附近水域进行鱼类采样.结果如下:(1)共获得86种鱼类,隶属于12目41科70属,其中鲈形目种类最多,占总数的51.2%,其次是鲱形目11种,其余各目均不超过6种;(2)采用Pinkas相对重要性指数划分标准,九龙江口浮宫附近水域的鱼类群落的全年优势种有4种,常见种9种,偶见种73种,其中优势种分别为裸头双边鱼(Ambassis gymnocephalus)、前鳞骨鲻(Osteomugil ophuyseni)、斑鰶(Konosirus punctatus)和青鳞小沙丁鱼(Sardinella zunasi),4个优势种的重量占渔获总重量的65.4%,其个体数占渔获总个体数的64.2%;(3)对鱼类群落结构起主导作用的种类有13种;(4)洄游性鱼类主要有龙头鱼(Harp-odon nehereus)、鳓(llisha elongata)及金色小沙丁鱼(Sardinella aurita)等,洄游至该水域的时期一般为夏季;(5)全年各月的Shannon-Wiener多样性指数变动范围为1.08 ~ 1.91,均匀度指数波动于0.36 ~0.56,而丰富度指数变化幅度为1.38 ~ 3.48.聚类分析结果显示,除1、2月间的相似性(72.5%)和10、11月的(62.1%)较高外,其余各月间的相似性均较低.以上结果表明,该水域的鱼类生物群落变动较大,具有较高的不稳定性.

  15. PENGARUH KATALIS BASA (NaOH PADA TAHAP REAKSI TRANSESTERIFIKASI TERHADAP KUALITAS BIOFUEL DARI MINYAK TEPUNG IKAN SARDIN

    Directory of Open Access Journals (Sweden)

    Diah Probo Ningtyas

    2013-06-01

    The research purpose has studied the influence of NaOH concentration in transesterification process and examinate its effect on the quality of biofuels production, conversion, and physic quality. The variables that analysed was the effect of NaOH concentration as catalyst (0.5%, 1.0%, 1.5%, and 2.0% from amount of oil and methanol in the transesterification reaction step. The result showed that the increasing NaOH concentration (0.5 - 1.5%, enhanced the biofuel conversion (%. The highest conversion of biofuels was achieved by using 1.50% NaOH (w/w with 45.34% biofuels conversion. The major component in the biofuels was methyl palmitate (20.31%. ASTM analysis data also supported that the biofuel product was in agreement with automotive diesel fuel specification.

  16. IMPLEMENTATION OF LEAN MANUFACTURING IN FISH CANNING COMPANY: A CASE STUDY OF A CANNED SARDINES PRODUCTION COMPANY IN MOROCCO

    Directory of Open Access Journals (Sweden)

    I. Idrıssi

    2015-12-01

    Full Text Available Lean is a powerful tool, which can bring significant benefit to manufacturing industries by creating value through reduction of waste. Although the lean concept has become very popular in mass production industries such as the automotive industry, more recently the concept has been adopted in different batch processing industries and service sectors. The application of lean tools into the food processing industry has not received the same level of attention compared to the traditional manufacturing industries. The paper discusses how the lean concept could be applied to a fish manufacturing company. The paper first presents the lean concept tools. The empirical section discusses how a case company, operating as a contract manufacturer in the food industry, has applied the lean production concept and tools. In the case study, three analysis tools are examined and the structures of demand chains of different customers are presented. The delivery times will decrease and more flexibility will be needed from the contract manufacturer. The case study shows that much movement is possible toward the lean supply chain and partnership-based cooperation. By implementing the lean concept, food companies can increase customer value through cost reduction or through provision of additional value-enhanced services.

  17. Addition of Ca-soap of Sardinella longiceps oil into the ration: 2. Its effect on physical and chemical characteristics of lamb

    Directory of Open Access Journals (Sweden)

    A Sudarman

    2008-06-01

    Full Text Available Lamb contains high saturated fatty acids and cholesterol. Consuming such meat tends to increase atherosclerosis incident. Fat content and composition of meat might be manipulated by giving lemuru fish oil which is rich in HDL and unsaturated fatty acids into sheep diet. Giving lemuru fish oil in the protected form, such as complex calcium-soap is believed to be more beneficial. Twelve sheep were distributed into four groups to receive four experimental diets in studying the influence of calcium soap on meat quality. The sheep were allocated based on Randomized Block Design and at the end of rearing period they were slaughtered. The experimental diets were: R0 = control diet, R1 = R0 + 1.5% calcium soap, R2 = R0 + 3% calcium soap and R3 = R0 + 4.5% calcium soap. Data were analyzed using analysis of variance and any significant differences were further tested using contrast orthogonal. The results showed that the treatments significantly (P<0.01 reduced fat content, both of low density lipoprotein cholesterol (LDL, and high density lipoprotein cholesterol (HDL of sheep meat. There were no differences in tendernes, cooking loss, water holding capacity (WHC and pH of meat. It is concluded that the best level of Ca-soap fish oil addition to yield healthy meat is 3%.

  18. Larval fish assemblages across an upwelling front: Indication for active and passive retention

    Science.gov (United States)

    Tiedemann, Maik; Brehmer, Patrice

    2017-03-01

    In upwelling areas, enrichment, concentration and retention are physical processes that have major consequences for larval fish survival. While these processes generally increase larval survival, strong upwelling can also increase mortality due to an offshore transport of larvae towards unfavorable habitats. In 2013 a survey was conducted along the Senegalese coast to investigate the upwelling effect with regard to larval fish assemblages and possible larval fish retention. According to water column characteristics two distinct habitats during an upwelling event were discriminated, i.e. the inshore upwelled water and the transition area over the deepest part of the Senegalese shelf. Along the two areas 42,162 fish larvae were collected representing 133 species within 40 families. Highest larval fish abundances were observed in the inshore area and decreasing abundances towards the transition, indicating that certain fish species make use of the retentive function of the inner shelf area as spawning grounds. Two larval fish assemblages overlap both habitats, which are sharply delimited by a strong upwelling front. One assemblage inhabited the inshore/upwelling area characterized by majorly neritic and pelagic species (Sparidae spp., Sardinella aurita), that seem to take the advantage of a passive retention on the shelf. The second assemblage consisted of a mix of pelagic and mesopelagic species (Engraulis encrasicolus, Carangidae spp. and Myctophidae spp.). Some species of the second assemblage, e.g. horse mackerels (Trachurus trachurus and Trachurus trecae), large finned-lantern fish (Hygophum macrochir) and foureyed sole (Microchirus ocellatus), revealed larval peak occurrences at intermediate and deep water layers, where the near-ground upwelling layer is able to transport larvae back to the shelf. This indicates active larval retention for species that are dominant in the transition area. Diel vertical migration patterns of S. aurita, E. encrasicolus and M

  19. Interaction between spawning habitat and coastally steered circulation regulate larval fish retention in a large shallow temperate bay

    Science.gov (United States)

    Álvarez, Itziar; Catalán, Ignacio A.; Jordi, Antoni; Alemany, Francisco; Basterretxea, Gotzon

    2015-12-01

    Larval retention plays a fundamental role in the persistence of coastal fish assemblages. Here, we examine larval fish distribution and abundance patterns in Palma Bay, a large (˜20 km) wind-driven microtidal bay in the southern coast of Mallorca (Spain, NW Mediterranean Sea). Larval fish assemblage structure in the bay were analyzed during July 2010 and interpreted in the context of the observed circulation patterns, adult habitat distribution and spawning traits. Acoustic Doppler Current Profiler (ADCP) observations showed the presence of retentive flow patterns in the middle of the bay enhancing local larval accumulation and self-recruitment. In consequence, larval abundances were higher in this central part of the bay (˜40 m depth, mean abundance 607.6 ± 383 ind. 10 m-2) than along the coastal fringe (RDA) revealed differences between the larval fish assemblages in areas inside the bay, constituted by small pelagic and benthopelagic taxa (gobids, Chromis chromis and Serranus hepatus) and offshore larvae, mostly from meso and large pelagic fish. These larval fish assemblages were structured according to depth variations and zooplankton abundance, and remained relatively unmixed because of the circulation patterns in the mouth of the bay that uncouple its dynamics from alongshelf circulation. Even larvae of typically pelagic species that spawn close to the coast (Sardinella aurita, Auxis rochei) were associated with the retentive effect of the bay. Our study highlights the important role of coastal bays in the regulation of coastal fish population dynamics and as hotspots for the maintenance of diversity in the Mediterranean Sea.

  20. Preliminary Evaluation on Resources Enhancement of Artificial Reef in the East Corner of Zhelang Shanwei

    Institute of Scientific and Technical Information of China (English)

    Lichen; FANG; Pimao; CHEN; Guobao; CHEN; Yong; TANG; Huarong; YUAN; Xue; FENG

    2013-01-01

    In order to analyze the improvement of fish resource,the trawl net and gill net were used in the background survey and investigation in the artificial reef area and control area of the east of corner was discussed in Zhelang Shanwei in January 2003 and April 2010 respectively.Margalef species richness index,Shannon-Winener diversity index and Pileou uniformity index were chosen to analyze the multiformity of biological population and community structure.Resource density index of trawl net investigation and catch rate of gill net were chosen to estimate the variance of biomass.The results of the trawl net showed that the resources density of the artificial area increased by 3.078 times,and numbers of species increased by 1.875 times,squilla being the dominant group instead of the fish,and the first dominant species being squilla oratoria(Oratosquilla oratoria),which increased by 4.372 times before the artificial reef dropped.The results of the gill net showed that the catch rate of the artificial area increased by 12.043 times,numbers of species increased by 1.333 times,the dominant group was fish,it increased greatly,the first dominant species was golden small fish(Sardinella aurita),which increased by 13.035 times before the artificial reef dropped.Both the results of trawl net and gill net indicated that the effect of fish assemblage were evident,and the community structure had improved greatly.It showed a wonderful effect on conservation and assemblage of fishes resource.The artificial reefs developed a new resource enhancement system in the east corner of Zhelang Shanwei accomplished basically.

  1. Molecular Detection of the Three Major Pathogenic Vibrio Species from Seafood Products and Sediments in Tunisia Using Real-Time PCR.

    Science.gov (United States)

    Gdoura, Morsi; Sellami, Hanen; Nasfi, Hanen; Trabelsi, Rahma; Mansour, Sabeur; Attia, Touraya; Nsaibia, Siwar; Vallaeys, Tatiana; Gdoura, Radhouane; Siala, Mariam

    2016-12-01

    Vibrio spp. have emerged as a serious threat to human health worldwide. V. parahaemolyticus , V. cholerae , and V. vulnificus pose a considerable public health risk in Tunisia because they cause sporadic and epidemic foodborne infections associated with the consumption of raw or undercooked contaminated seafood. More recently, toxR-positive V. alginolyticus was also reported to be a potential source of contaminated seafood. A total of 247 samples, including 113 fishes ( Labrus viridis , Penaeus kerathurus , Diplodus annularis , Diplodus sparaillon , Scorparna porcus , Sarpa salpa , Dentex dentex , Scorparna scrofa , Sardinella aurita , Trachurus trachurus , Synodus saurus , Pagellus erythrinus , and Metapenaeus monoceros ), 83 clams ( Ruditapes decussatus species), 30 seawater samples, and 21 sediment samples were analyzed using traditional culture methods (ISO/TS 21872-1; International Organization for Standardization 2007) and a conventional PCR method for Vibrio spp. A rapid, sensitive, and highly reproducible real-time PCR assay was developed to detect the three major Vibrio spp. pathogenic for humans in Tunisian seafood products and sediments. A conventional culture method found 102 (41.3%) of 247 analyzed samples positive for Vibrio spp.; a conventional PCR method found 126 (51%) of the 247 samples positive. Real-time PCR assay found 126 (51.1%) samples positive; V. alginolyticus toxR was the most common, found in 99 (78.57%) of samples, followed by V. parahaemolyticus in 26 (20.63%) and V. cholerae in 1 (0.7%). All culture-positive samples were PCR positive. However, 24 samples that were positive by conventional PCR and real-time PCR were culture negative. Our findings indicate that retail seafood is commonly contaminated with Vibrio spp. and presents a potential risk to human health in Tunisia. These data also indicate that real-time PCR can provide sensitive species-specific detection of Vibrio spp. in seafood without prior isolation and characterization

  2. Mercury speciation in fish of the Cabo Frio upwelling region, SE-Brazil

    Directory of Open Access Journals (Sweden)

    Carlos Alberto da Silva

    2011-09-01

    Full Text Available Mercury distribution in the oceans is controlled by complex biogeochemical cycles, resulting in retention of trace amounts of this metal in marine biota. The impact of upwelling processes in this metal behavior has been overlooked. Data from literature are insufficient to evaluate the risks associated with the presence of mercury in the fish collected in upwelling areas and its consumers. Therefore, the aim of the present work was to perform a study of mercury speciation in four fish species belonging to different trophic levels from Cabo Frio-Brazil upwelling region. The total mercury content vary of 53 ng g-1 (Sardinella brasiliensis -sardine to 1215 ng g-1 (Cynoscion striatus -striped weakfish and, with exception of the planktivorous fish, methylmercury levels reaches circa 90% of total mercury concentration.A distribuição de Mercúrio nos oceanos é controlada por um complexo ciclo biogeoquímico, resultando na retenção de pequenas quantidades na biota marinha. O impacto dos processos de ressurgência costeira no comportamento desse metal tem sido negligenciado. Dados da literatura são insuficientes para elucidar o risco associado com a presença de mercúrio em peixes capturados em áreas de ressurgência e seus consumidores. Portanto o objetivo do presente trabalho foi realizar um estudo de especiação de mercúrio em quatro espécies de peixes pertencentes a diferentes níveis tróficos da região de ressurgência de Cabo Frio-Brasil. O conteúdo total de mercúrio variou de 53 ng g-1 (Sardinella brasiliensis -sardinha to 1215 ng g-1 (Cynoscion striatus -pescada e, com exceção da espécie planctivora, os níveis de metilmercúrio atingem cerca de 90% da concentração total de mercúrio.

  3. ANALISIS KERENTANAN JENIS IKAN PELAGIS KECIL DI PERAIRAN SELAT BALI DAN SELAT MAKASSAR TERHADAP DINAMIKA SUHU PERMUKAAN LAUT

    Directory of Open Access Journals (Sweden)

    Reny Puspasari

    2016-09-01

    Full Text Available Kondisi oseanografi Indonesia dipengaruhi oleh dinamika proses oseanografi global. Salah satu proses tersebut adalah Arlindo yang menghantarkan massa air bersuhu hangat dari Samudera Pasifik ke Samudera Hindia melalui Selat Makassar. Dinamika suhu perairan akan mempengaruhi kondisi sumberdaya ikan di perairan tersebut. Penelitian bertujuan untuk menganalisis dampak dinamika suhu permukaan laut terhadap empat jenis ikan pelagis yaitu ikan layang biru (Decapterus macarellus, kembung (Ratrelliger kanagurta, lemuru (Sardinella lemuru dan tongkol (Auxis thazard.  Penelitian dilakukan pada tahun 2015 dengan membuat profil biologis setiap jenis ikan, kemudian dilakukan penilaian oleh para ahli mengenai kerentanan setiap jenis ikan terhadap paparan dinamika SPL.  Hasil penilaian para ahli kemudian dianalisis menggunakan metode kerentanan jenis yang digunakan oleh NOAA.  Hasil analisis menunjukkan bahwa empat jenis ikan pelagis yang dianalisis mempunyai tingkat kerentanan yang rendah terhadap paparan dinamika suhu permukaan laut.  Tingkat kerentanan ikan pelagis kecil di perairan Selat Bali lebih tinggi bila dibandingkan dengan Selat Makassar.   Oceanographic condition of Indonesian waters is highly influenced by global oceanographic processes. Indonesian through flow is one of the global oceanographic processes that affect the oceanographic condition in Indonesia waters, it delivers warm water from Pacific Ocean to Indian Ocean through the strait of Makassar. Therefore, the dynamic of sea water temperature will affect the fish resources condition. The objective of this study is to analyze the impact of the dynamic of sea surface temperature to pelagic fishes such as mackerel scad (Decapterus macarellus, Indian mackerel (Ratrelliger. kanagurta, Bali sardine (Sardinella. lemuru and frigate tuna (Auxis. thazard. The study was conducted in 2015 consists of three phases of process, describing biological profile of the species, then scoring based on

  4. Biological Characteristics of Moon Jellyfish(Aurelia aurita Linnaeus,1758) and Its Bloom%海月水母的生物学特征及其爆发

    Institute of Scientific and Technical Information of China (English)

    郑凤英; 陈四清; 倪佳

    2010-01-01

    介绍了海月水母的形态、生活习性、生活史、分布、生存条件、爆发及危害等基本情况,并分析了其爆发成因,最后提出了我国海月水母及其爆发研究的工作重点:确认在我国海域爆发的海月水母究竟是外来种还是本地种,了解其种群的生活史、食性及生活习性,掌握其种群数量动态变化规律并进行数量动态监测,分析其种群数量动态与海域环境的关系,开展其爆发的危害评价工作.

  5. Factors influencing the asexual reproduction of moon jellyfish Aurelia aurita%海月水母无性生殖的影响因子

    Institute of Scientific and Technical Information of China (English)

    付瑶; 董志军; 刘东艳

    2012-01-01

    海月水母是我国黄渤海近岸海域的常见水母种类,近年来海月水母的暴发性增殖已经成为一种近海生态灾害.论文综述了海月水母无性生殖过程的主要类型,其中出芽生殖和横裂生殖是海月水母无性生殖的主要方式.并对影响海月水母无性生殖的主要因子进行了分析和总结,指出现有研究中存在的问题,为进一步开展海月水母无性生殖机制研究提供参考.

  6. A method for in situ estimation of prey selectivity and predation rate in large plankton, exemplified with the jellyfish Aurelia aurita (L.)

    DEFF Research Database (Denmark)

    Hansson, L.J.

    2006-01-01

    among individual jellyfish and among the various oral arms and gastric pouches within individuals. Clearance rates varied strongly with prey type. The medusae selected large crustacean prey (cladocerans and copepods/copepodites) over echinoderm larvae and copepod nauplii. Prey distribution within...

  7. The role of Odontella aurita, a marine diatom rich in EPA, as a dietary supplement in dyslipidemia, platelet function and oxidative stress in high-fat fed rats

    National Research Council Canada - National Science Library

    Haimeur, Adil; Ulmann, Lionel; Mimouni, Virginie; Guéno, Frédérique; Pineau-Vincent, Fabienne; Meskini, Nadia; Tremblin, Gérard

    2012-01-01

    Dietary changes are a major factor in determining cardiovascular risk. n-3 polyunsaturated fatty acids modulate the risk factors for metabolic syndrome via multiple mechanisms, including the regulation of the lipid metabolism...

  8. Spatial distribution and dietary overlap between Japanese anchovy Engraulis japonicus and moon jellyfish Aurelia aurita in the Seto Inland Sea, Japan

    Directory of Open Access Journals (Sweden)

    Jun Shoji

    2009-10-01

    Full Text Available Biological and physical surveys were conducted in order to investigate the relationship between environmental conditions and the distribution of ichthyoplankton and jellyfish, and dietary overlap between the ichthyoplankton and jellyfish in the Seto Inland Sea (SIS, Japan. Ichthyoplankton, copepods, and jellyfish were collected during two cruises in July 2005 in the Sea of Hiuchi and in July 2006 in Hiroshima Bay within the SIS. Sea surface temperature (˚C, salinity, bottom-layer dissolved oxygen (mg l-1 and the abundance (no. m-2 of fish eggs and larvae were significantly higher in the Sea of Hiuchi. Japanese anchovy was most dominant (69.3% in number of eggs and 52.3% in number of larvae among the ichthyoplankton. Mean jellyfish biomass (g m-2 in Hiroshima Bay was significantly higher (50-folds than that in the Sea of Hiuchi. Moon jellyfish was the most dominant among the jellyfish collected, accounting for 85.6% in wet weight. Surface temperature had a significant effect on fish egg and larval distribution: abundance of fish eggs and larvae increased with increasing temperature. Jellyfish abundance was negatively correlated with the bottom-layer oxygen concentration. Stable isotope analysis indicated dietary overlap between the Japanese anchovy and the moon jellyfish in Hiroshima Bay.

  9. Where are the polyps? Molecular identification, distribution and population differentiation of Aurelia aurita jellyfish polyps in the southern North Sea area

    NARCIS (Netherlands)

    Walraven, van L.; Driessen, F.; Bleijswijk, van J.; Bol, A.; Luttikhuizen, P.; Coolen, J.W.P.; Bos, Oscar; Gittenberger, A.; Schrieken, N.; Langenberg, V.T.; Veer, van der H.W.

    2016-01-01

    For many species of metagenic jellyfish the location of the benthic polyps is unknown. To gain insight in the distribution, species composition and population structure of scyphozoan jellyfish polyps in the southern North Sea area, polyp samples were collected from natural and artificial substrates

  10. 2013 Joint U.S.-Canada Integrated Acoustic and Trawl Survey of Pacific Hake and Pacific Sardine (SaKe 2015) (SH1507, ME70)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The 2015 SaKe survey will span the west coasts of the U.S. and Canada from approximately lat 32.8?N (San Diego) to approximately lat 54.65?N (Dixon Entrance,...

  11. 2013 Joint U.S.-Canada Integrated Acoustic and Trawl Survey of Pacific Hake and Pacific Sardine (SaKe 2015) (SH1507, EK60)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The 2015 SaKe survey will span the west coasts of the U.S. and Canada from approximately lat 32.8?N (San Diego) to approximately lat 54.65?N (Dixon Entrance,...

  12. 2013 Joint U.S.-Canada Integrated Acoustic and Trawl Survey of Pacific Hake and Pacific Sardine (SaKe 2013) (SH1305, EK60)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Scientists from the Fishery Resource Analysis and Monitoring (FRAM) division at the NOAA Fisheries Northwest Fisheries Science Center (NWFSC), the Fishery Resources...

  13. The influence of industrial-scale canning on cadmium and lead levels in sardines and anchovies from commercial fishing centres of the Mediterranean Sea.

    Science.gov (United States)

    Galitsopoulou, Augoustina; Georgantelis, Dimitrios; Kontominas, Michael

    2012-01-01

    The current study encompassed a survey on the levels of toxic trace elements in two highly consumed fish species in commercial fishing centres of western, central and eastern Mediterranean Sea. A Zeeman GTA-AAS graphite furnace atomic absorption spectrometry system was used throughout the study. Toxicological evaluation of the samples revealed a low Cd content in the raw samples, ranging between 0.003 and 0.027 mg kg⁻¹. Pb presented significantly higher values, from 0.037 to 0.297 mg kg⁻¹, occasionally reaching the limit of 0.3 mg kg⁻¹. Heavy metal levels were particularly higher in bones, thus raising queries about the safe consumption of fish intended to be eaten as a whole, a very common practice for small fish and canned products. The influence of industrial-scale canning showed that canning enhanced heavy metal levels by 35%-80%. The effect of canning depended on metal type and reduction of moisture loss after the steam-roasting step of the canning procedure.

  14. 2012 Joint U.S.-Canada Integrated Acoustic and Trawl Survey of Pacific Hake (Merluccius productus) and Pacific Sardine (Sardinops sagax) (SH1204, EK60)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Scientists from the Fishery Resource Analysis and Monitoring (FRAM) division at the NOAA Fisheries Northwest Fisheries Science Center (NWFSC), the Fishery Resources...

  15. The 2012 Joint U.S.-Canada Integrated Acoustic and Trawl Survey of Pacific Hake (Merluccius productus) and Pacific Sardine (Sardinops sagax) (SH1204, ME70)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Scientists from the Fishery Resource Analysis and Monitoring (FRAM) division at the NOAA Fisheries Northwest Fisheries Science Center (NWFSC), the Fishery Resources...

  16. 九种鲱科鱼类线粒体DNA细胞色素b基因相对速率测试及分歧时间估计%Phylogenetic analysis and relative-rate test of nine Clupeidae fishes ( Osteichthyes: Clupeiformes) inferred from cytochrome b gene sequence of mitochondrial DNA

    Institute of Scientific and Technical Information of China (English)

    程起群; 卢大儒

    2006-01-01

    对鲱科9种鱼类的线粒体细胞色素b(cytb)基因序列进行综合分析.结果表明:(1)用NeighbourJoining(NJ)法构建的分子系统树显示盖纹沙丁鱼属的3种鱼类形成一个单系类群,鲱属2种鱼类形成一个单系类群,它们的bootstrap支持率均为100%.金色小沙丁鱼和短体小沙丁鱼形成一个单系类群,在NJ树中的支持率达到或超过50%.欧洲黍鲱与太平洋鲱和大西洋鲱是单系起源,其bootstrap支持率高达100%.系统发育结果还支持沙丁鱼属鱼类与盖纹沙丁鱼属鱼类是单系类群,支持率分别为87%和95%.但单系类群Ⅰ(包括大西洋鲱、太平洋鲱、欧洲黍鲱)和单系类群Ⅱ(包括欧洲沙丁鱼、加州沙丁鱼、南美拟沙丁鱼、斑点盖纹沙丁鱼)之间的系统发育关系尚不明确,因为它们之间的支持率很低.(2)dN/dS的比值显著的小于1(Ztest),提示由于功能限制,cytb基因受到强烈的负选择作用.(3)基于Tajima的1D和2D相对速率测试表明,分子钟假说在鲱科鱼类中是成立的;鲱科鱼类的分歧时间是0.12至13.45百万年间.%Phylogenetic analysis and relative- rate test of 9 Clupeidae fishes were performed using the cytochrome b(cytb) gene sequence data. Based on Kimura-2-Parameter(K2P) distance, bootstrapped NJ tree ( 1000 replicates) was generated from these 9 cytb gene sequences of Clupeidae fishes. Three species of genus Sardinops are monophyletic group, 2 species of genus Clupea are monophyletic group, and their bootstrap values are both 100%. Sardinella aurita and Sardinella maderensis are monophyletic group, their bootstrap value reached or exceed 50%. Sprattus sprattus which is a species of genus Sprattus, has a monophyletic origin with genus Clupea, their bootstrap value was 100%. The number of synonymous nucleotide substitution per synonymous site (dS) and the number of nonsynonymous nucleotide substitution per nonsynonymous site (dN) of cytb gene among Clupeidae fishes were calculated

  17. Composição em ácidos graxos de líquidos de cobertura de sardinhas brasileiras (Sardinella brasiliensis em conserva - DOI: 10.4025/actascitechnol.v26i1.1535

    Directory of Open Access Journals (Sweden)

    César Ricardo Tarley

    2004-03-01

    Full Text Available O objetivo deste trabalho foi avaliar a composição em ácidos graxos nos líquidos de cobertura com óleo de soja ou molho de tomate de diferentes marcas de sardinhas comercializadas no Brasil. Os teores percentuais dos ácidos graxos EPA e DHA em óleo de soja como líquido de cobertura variaram de 0,95 a 3,96% e 0,63 a 1,09%, respectivamente. Para a cobertura molho de tomate os resultados indicaram que as migrações dos ácidos graxos EPA e DHA foram superiores em relação à cobertura em óleo de soja. Os percentuais encontrados para os ácidos graxos EPA e DHA na cobertura molho de tomate variaram de 2,95 a 13,29% e 1,25 a 4,67%, respectivamente. Os resultados indicaram que há diferenças em relação à qualidade das sardinhas utilizadas bem como no processamento. Embora exista diferença entre as duas formulações, com relação à composição de ácidos graxos, ambas podem ser consideradas boas fontes de ácidos graxos, principalmente os ácidos ômega-3

  18. [Detection of the mitochondrial DNA haplotype characteristic of the least cisco (Coregonus sardinella, Valenciennes, 1848) in the vendace (C. albula, Linnaeus, 1758) population of Vodlozero (the Baltic Sea basin)].

    Science.gov (United States)

    Borovikova, E A; Makhrov, A A

    2009-01-01

    Analysis of the nucleotide sequence of the mitochondrial ND-1 gene in the vendace population in lake Vodlozero (the eastern part of the Baltic Sea basin) revealed a sequence variant that is closely related to that of the least cisco of Siberia (the Indigirka River). Thus, together with the results of morphological and allozyme analysis of this population performed earlier, the results obtained in this study are suggestive of the immigration of the least cisco to the Baltic Sea basin during the last glaciation.

  19. 汕尾遮浪角东人工鱼礁区渔业资源变动分析%Improvement analysis on fisheries resources in artificial reef area of the east of Zhelang corner, Shanwei city

    Institute of Scientific and Technical Information of China (English)

    房立晨; 陈丕茂; 陈国宝; 汤勇; 袁华荣; 冯雪

    2012-01-01

    为了解汕尾遮浪角东人工鱼礁区渔业资源变动情况,分别于2003年1月和2010年4月使用拖网和刺网对礁区和对比区进行了本底调查和跟踪调查,使用Margalef种类丰富度指数、Shannon-Winener多样性指数和Pileou均匀度指数分析游泳生物多样性和群落结构变动情况,使用拖网调查资源密度指数和刺网调查渔获率指数分析渔业资源生物量的变动情况.拖网调查结果显示,投礁后礁区游泳生物资源密度是投礁前的3.078倍,种类数是投礁前的1.875倍,第一优势种口虾蛄(Oratosquilla oratoria)的资源密度是投礁前第一优势种叫姑鱼(Johnius dussumieri)的4.372倍.刺网调查结果显示,投礁后礁区游泳生物渔获率是投礁前的12.043倍,渔获种数是投礁前的1.333倍,礁区海域优势类群鱼类的渔获率显著提高,第一优势种金色小沙丁鱼(Sardinella aurita)的渔获率是投礁前第一优势种黄斑蓝子鱼(Siganus oramin)的13.035倍.拖网调查和刺网调查均表明,建礁后游泳生物的诱集效果明显,生物群落结构得到改善,资源量显著增加,表现出良好的资源聚集效果,形成了新的人工鱼礁增殖系统.

  20. PATTERNS OF PREY BIOMASS CONSUMPTION BY SMALL ODONTOCETES IN THE NORTHEASTERN COAST OF VENEZUELA

    Directory of Open Access Journals (Sweden)

    Lenin E. Oviedo Correa

    2011-07-01

    Full Text Available Trophic relationships are conditioned by population dynamics of interacting species in the community (species present, food web connections among them, and the strength of interactions, and on the consequences of these species interactions depend various ecosystem processes such as productivity and nutrient flux. Odontocetes target a wide range of prey items and are adapted to feeding at different depths. The aim of this report is to describe the patterns of prey consumption by small odontocetes, incorporating natural predatory patterns into a potential management scheme of strategic food sources, for both human and marine predators. Using the geo-statistical analysis tool of ArcGIS 9.2, maps illustrating the intensity and location of prey consumption were made for species with a sighting index (SPUE > 0.15. The biomass consumption emphasized the differences in habitat use by species. The trends in distribution of prey biomass removal by odontocetes particularly suggest a stratification of prey consumption primarily in shelf waters, with a prey biomass that is comprised basically by demersal fish and small pelagics (including Sardinella aurita, and into transition-oceanic depths where most of the predatory pattern would potentially rely on pelagic - mesopelagic squid and myctophids. Overall the spatial tendencies in regionalization presented in this contribution will serve as a base-line to assess ecosystem health and evaluate management scenarios.   Las relaciones tróficas son reguladas por la dinámica poblacional de las especies que interactúan dentro de la comunidad (especies presentes, conexiones interespecíficas, y nivel de interacción y de las consecuencias de esas interacciones para procesos del ecosistema como la productividad y el flujo de nutrientes. El objetivo de este reporte es evaluar el patrón de consumo de biomasa por odontocetos de menor tamaño en la costa Nororiental de Venezuela, incorporando patrones de depredaci

  1. Concentração e retenção do selênio em peixes marinhos Concentration and retention of selenium in marine fish

    Directory of Open Access Journals (Sweden)

    Alfredo Tenuta Filho

    2010-05-01

    average levels of Se found in bony fish, gutted corvina (Micropogonias furnieri, triggerfish (Balistes capricus, hake and sardine (Sardinella brasiliensis, 0.48 ± 0.18 μg.g-1, and in cartilaginous fish, small shark (squantina Guggenheim, hammerhead shark, spinner shark, and sand tiger shark, 0.36 ± 0.07 μg.g-1, did not differ from one another (p > 0.05. In fact, their occurrence was in accordance with the literature data on marine fish. The levels of Se found in the sharks (predatory species are not enough to establish a stoichiometric relation for the interaction with Hg focusing on the desired toxic protection. The effective Se retention evaluated in the sand tiger shark, after extraction with water, was considered high (minimum of 68.81% and important taking nutritional and toxicological aspects into account.

  2. 中西医结合治疗急性海月水母蜇伤临床研究%Clinical study of treating 136 cases with aurelia aurita sting by combination of Traditional Chinese Medicine and Western Medicine

    Institute of Scientific and Technical Information of China (English)

    邵月竹; 权正涛; 殷淑兰; 王进荣; 肖雪霞; 侯彩霞; 马丽状

    2001-01-01

    [目的]探讨中西医结合治疗海月水母蜇伤的方法.[方法]分析1997~1999年6~10月份收治的沿海渔民及加工者和海水游泳时被海月水母蜇伤的136例,分别采用中西医结合治疗,观察其临床疗效.[结果]治愈134例,占98.53%,死亡2例,占1.47%,总有效率为98.53%,对于海月水母蜇伤的主要症状改善时间明显优于单纯西药治疗.[结论]中西医结合的综合性治疗方法可显著提高临床疗效,有研究价值.

  3. 饵料浮游动物种类和密度对海月水母摄食和生长的影响%Effect of zooplanktonter species and density on feeding and growth in jellyfish Aurelia aurita

    Institute of Scientific and Technical Information of China (English)

    杨为东; 赵文; 李多慧; 滕力平; 杨担光; 袁霞; 韩婷婷

    2010-01-01

    研究了5种浮游动物(卤虫Artemia无节幼体、大尾猛水蚤Harpacticus uniremis 、短角异剑水蚤Apocyclops royi、蒙古裸腹溞Moina mongolica 和褶皱臂尾轮虫Brachionus plicatilis)饵料对海月水母Aurelia aurita摄食和碟状体生长的影响.结果表明:海月水母摄食卤虫无节幼体没有明显的节律性,对蒙古裸腹溞、大尾猛水蚤、短角异剑水蚤和褶皱臂尾轮虫都是在上午8:00摄食量最大;海月水母对卤虫无节幼体和轮虫的个体摄食率最大,分别为42.8、42.0 个/(个·h).摄食卤虫无节幼体的海月水母碟状体的生长率最大,达到8.8%~11.2%;摄食蒙古裸腹溞和大尾猛水蚤的次之,生长率分别为4.4%~6.1%和3.1%~5.2%;摄食褶皱臂尾轮虫的碟状体生长率极低,仅为0.3%~0.4%.研究表明,卤虫无节幼体、蒙古裸腹溞和大尾猛水蚤均可作为海月水母碟状体的饵料,合适投喂密度分别为550~700、150~300个/L和250~450个/L.

  4. Immunochemical analyses of soluble lens proteins in some marine fishes

    Digital Repository Service at National Institute of Oceanography (India)

    Menezes, M.R.

    Soluble eye lens proteins of 10 fishes, belonging to the families Clupeidae, Hemirhamphidae, Lactaridae, Scombridae, Stromatidae, Psettodidae, Bothidae and Soleidae were studied by immunoelectrophoresis using the lens antiserum of Sardinella...

  5. Variations in stomach contents and biochemical composition of tissues in some marine fishes

    Digital Repository Service at National Institute of Oceanography (India)

    Jacob, P.G.; Rajagopal, M.D.

    Relationship between calorific values and protein, fat and carbohydrate of the stomach contents (food) and body tissues of marine fishes, Sardinella longiceps, Valenciennes, Rastrelliger kanagurta (Cuvier), Otolithus ruber (Schneider) and Lactarius...

  6. Genetic diversity in the mtDNA control region and population ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-12-17

    Dec 17, 2008 ... Sardinella zunasi is a pelagic and subtropical fish species ... often influenced by the ocean currents and Pleistocene ice ages. ... influence population structure. ..... geographical isolation and sea environment such as water.

  7. Microbial quality and safety of fresh and dried Rastrineobola argentea from Lake Victoria, Tanzania

    DEFF Research Database (Denmark)

    Baniga, Zebedayo; Dalsgaard, Anders; Mhongole, Ofred J

    2017-01-01

    Rastrineobola argentea (silver cyprinid/sardines) is an important commercial fish species found in Lake Victoria. It is an important protein source for consumers in several African countries including Tanzania. A cross-sectional study was conducted using standard methods to assess...... the bacteriological quality of fresh and dried sardines from Lake Victoria. Sardine samples were randomly collected from main landing sites and retail markets in the city of Mwanza. Total viable counts (TVC) in fresh sardines were in the range of 5.18–7.90 log10 cfu/g while those dried on racks contained 3...... sites contained 30% (n = 20) and those from the markets contained 15% (n = 20) Salmonella spp. Salmonella spp. were not detected in sardines dried on racks. To conclude, sun drying of sardines on racks is an effective drying method providing a safe product for human consumption....

  8. Cytological observations on ferns from New Guinea with descriptions of new species

    NARCIS (Netherlands)

    Holttum, R.E.; Roy, S.K.

    1966-01-01

    Dicranopteris linearis var. montana Holtt. abnormal Microlepia strigosa (Thunb.) Presl Pseudophegopteris aurita (Hk.) Ching cyclocarpa Holttum Pseudocyclosorus caudatus Holttum petrophila (Copel.) Holttum Mesoneuron wantotensis (Copel.) Holttum Cyclosorus dentatus (Forsk.) Ching confertus (Brause) C

  9. 76 FR 4854 - Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Annual Specifications

    Science.gov (United States)

    2011-01-27

    ... as monitored stocks (jack mackerel, northern anchovy, and market squid). During public meetings each... sardine are landed with other CPS so as to minimize the targeting of Pacific sardine. In the event that an... greatly on market forces within the fishery, and on the regional availability of the resource to...

  10. Bioacoustic Absorption Spectroscopy

    Science.gov (United States)

    2016-06-07

    frequencies (Ching and Weston, 1971). RESULTS Measured resonance frequencies of absorption lines, which were attributed to adult (~ 1.3 khz) and juvenile ...of adult and juvenile sardines. These results suggest that bioacoustic absorption spectroscopy measurements permit isolation of juvenile from adult...from broadband tomographic transmission loss measurements over large areas . 2. Depths of sardines and contours of phytoplankton concentrations vs. time

  11. Many small pelagic fish species spawn in areas where their eggs ...

    African Journals Online (AJOL)

    spamer

    interaction of upwelling plumes and shelf waves ... 9: Size frequency distributions of (a–c) anchovy larvae and (d–f) sardine larvae in the inshore, midshore ... Crawford. (1981) reported that, before the collapse of the sardine resource in ...

  12. Facts about Vitamin D

    Science.gov (United States)

    ... Gorse/iStock/Thinkstock.com Food Eggs, sardines, and salmon contain vitamin D. Most fluid milk and some brands of ... 2. Typical Vitamin D Content in Food Food Vitamin D in IU (mcg) Salmon, cooked, 3 oz. 320 (8) Sardines, canned in ...

  13. 75 FR 1745 - Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Annual Specifications

    Science.gov (United States)

    2010-01-13

    ... comments will be accepted in Microsoft Word, Excel, WordPerfect, or Adobe PDF file formats only. Copies of... for actively managed species (Pacific sardine and Pacific mackerel) are based on formulas applied to... ``Assessment of Pacific Sardine Stock for U.S. Management in 2010'' (see ADDRESSES). The formula in the CPS...

  14. [Determination of histamine content in canned fish products determined by the colorimetry method of Hardy and Smith].

    Science.gov (United States)

    Windyga, B; Grochowska, A; Sciezyńska, H; Górecka, K; Fonberg-Broczek, M

    1992-01-01

    The content of histamine in 79 samples of imported canned fish products (sardines, mackerel) was determined. Histamine was assayed by colorimetric method of Hardy and Smith. In 18% of tested products (sardines) levels of histamine were exceeded 20 mg/100 of product. Usefulness of this method in routine quantitative determination of histamine content in canned fish products was documented.

  15. How sailfish use their bills to capture schooling prey

    DEFF Research Database (Denmark)

    Domenici, P.; Wilson, A.D.M.; Kurvers, R.H.J.M.

    2014-01-01

    on prey. Here, we present the first unequivocal evidence of how the bill is used by Atlantic sailfish (Istiophorus albicans) to attack schooling sardines in the open ocean. Using high-speed video-analysis, we show that (i) sailfish manage to insert their bill into sardine schools without eliciting...

  16. A matter of tolerance: Distribution potential of scyphozoan polyps in a changing environment

    DEFF Research Database (Denmark)

    Goldstein, Josephine; Augustin, Christina B.; Bleich, Steffen

    2017-01-01

    Jellyfish blooms are unpredictable, unsustainable events, frequently affecting aquatic ecosystems severely. Of particular interest are the consequences of environmental change for jellyfish populations, especially in semi-enclosed habitats. Regional and seasonal changes in water chemistry...... psu caused irreversible degeneration of A. aurita and C. capillata polyps, respectively. Observed physiological limits suggest distribution of polyp populations of A. aurita in central and of C. capillata western parts of the Baltic Sea, while future climate changes may particularly restrict...

  17. Fish Oil and Microalga Omega-3 as Dietary Supplements: A Comparative Study on Cardiovascular Risk Factors in High-Fat Fed Rats.

    Science.gov (United States)

    Haimeur, Adil; Mimouni, Virginie; Ulmann, Lionel; Martineau, Anne-Sophie; Messaouri, Hafida; Pineau-Vincent, Fabienne; Tremblin, Gérard; Meskini, Nadia

    2016-09-01

    Dietary supplementation with marine omega-3 polyunsaturated fatty acids (n-3 PUFA) can have beneficial effects on a number of risk factors for cardiovascular disease (CVD). We compared the effects of two n-3 PUFA rich food supplements (freeze-dried Odontella aurita and fish oil) on risk factors for CVD. Male rats were randomly divided into four groups of six animals each and fed with the following diets: control group (C) received a standard diet containing 7 % lipids; second group (HF high fat) was fed with a high-fat diet containing 40 % lipids; third group (HFFO high fat+fish oil) was fed with the high-fat diet supplemented with 0.5 % fish oil; and fourth group (HFOA high fat+O. aurita) received the high-fat diet supplemented with 12 % of freeze-dried O. aurita. After 8 weeks rats fed with the high-fat diet supplemented with O. aurita displayed a significantly lower bodyweight than those in the other groups. Both the microalga and the fish oil significantly reduced insulinemia and serum lipid levels. O. aurita was more effective than the fish oil in reducing hepatic triacyglycerol levels and in preventing high-fat diet-induced steatosis. O. aurita and fish oil also reduced platelet aggregation and oxidative status induced by high fat intake. After an OA supplementation, the adipocytes in the HFOA group were smaller than those in the HF group. Freeze-dried O. aurita showed similar or even greater biological effects than the fish oil. This could be explained by a potential effect of the n-3 PUFA but also other bioactive compounds of the microalgae.

  18. Breve revisión bibliográfica de los muestreos de ictioplancton realizados en los sectores norte y sur del mar de Alborán, con atención especial en los que aportan información sobre el boquerón (E. encrasicolus) y la alacha (S. aurita).

    OpenAIRE

    Pérez-de-Rubín-y-Feigl, J. (Juan)

    1994-01-01

    A la breve exposición de los resultados obtenidos por los diferentes autores se añade un resumen gráfico de las coberturas espacial y temporal de los 20 muestreos ictioplanctonicos registrados hasta 1992, inclusive. A brief presentation is given of the results obtained by different authors, together with a graphic summary of the spatial and temporal coverage of the 20 icthyoplankton sampling registered up to and including the year 1992. Postprint

  19. Description of Karaganops n. gen. perratus (Daniltshenko 1970) with otoliths in situ, an endemic Karaganian (Middle Miocene) herring (Clupeidae) in the Eastern Paratethys

    DEFF Research Database (Denmark)

    Baykina, Eugenia M.; Schwarzhans, Werner W.

    2017-01-01

    Karaganops is established as a new fossil genus of the family Clupeidae, subfamily Clupeinae, to encompass the nominal species Sardinella perrata Daniltshenko 1970 from the Karaganian of southern Russia. Karaganops belongs to the group of genera characterized by the presence of two elongated rays...

  20. Browse Title Index

    African Journals Online (AJOL)

    Items 201 - 250 of 333 ... Vol 9, No 1 (2010), Market supply response of imported sardinella in Cross River State, Nigeria, Abstract ... Vol 6, No 1 (2007), Multivariate evaluation of variation in ... peasant agriculture: An application of data envelopment analysis ... Vol 4, No 1 (2005), Planning sustainable soil management under ...

  1. Germinação de sementes de Bothriospora corymbosa (Rubiaceae) recuperadas do trato digestório de Triportheus angulatus (sardinha) no Lago Camaleão, Amazônia Central Seed germination of Bothriospora corymbosa (Rubiaceae) recouped of digestive tract of Triportheus angulatus (sardine) in Camaleão Lake, Central Amazonian

    OpenAIRE

    Lúcia Alencar Maia; Leocinira M. dos Santos; Pia Parolin

    2007-01-01

    Na Amazônia as áreas inundáveis são cobertas por florestas com alta diversidade de espécies arbóreas que proporcionam a ictiofauna frutos e sementes indispensáveis a sua alimentação. Alguns estudos de alimentação têm mostrado que a estrutura tegumentar de sementes encontradas no trato digestório de peixes, se apresentavam intactas. Assim, este estudo teve como objetivo verificar se Triportheus angulatus (Spix & Agassiz, 1829) pode ser um agente dispersor de Bothriospora corymbosa (Bth) Hook. ...

  2. Genetic signs of multiple colonization events in Baltic ciscoes with radiation into sympatric spring- and autumn-spawners confined to early postglacial arrival.

    Science.gov (United States)

    Delling, Bo; Palm, Stefan; Palkopoulou, Eleftheria; Prestegaard, Tore

    2014-11-01

    Presence of sympatric populations may reflect local diversification or secondary contact of already distinct forms. The Baltic cisco (Coregonus albula) normally spawns in late autumn, but in a few lakes in Northern Europe sympatric autumn and spring- or winter-spawners have been described. So far, the evolutionary relationships and taxonomic status of these main life history forms have remained largely unclear. With microsatellites and mtDNA sequences, we analyzed extant and extinct spring- and autumn-spawners from a total of 23 Swedish localities, including sympatric populations. Published sequences from Baltic ciscoes in Germany and Finland, and Coregonus sardinella from North America were also included together with novel mtDNA sequences from Siberian C. sardinella. A clear genetic structure within Sweden was found that included two population assemblages markedly differentiated at microsatellites and apparently fixed for mtDNA haplotypes from two distinct clades. All sympatric Swedish populations belonged to the same assemblage, suggesting parallel evolution of spring-spawning rather than secondary contact. The pattern observed further suggests that postglacial immigration to Northern Europe occurred from at least two different refugia. Previous results showing that mtDNA in Baltic cisco is paraphyletic with respect to North American C. sardinella were confirmed. However, the inclusion of Siberian C. sardinella revealed a more complicated pattern, as these novel haplotypes were found within one of the two main C. albula clades and were clearly distinct from those in North American C. sardinella. The evolutionary history of Northern Hemisphere ciscoes thus seems to be more complex than previously recognized.

  3. Nutrition and Myasthenia Gravis

    Science.gov (United States)

    ... calcium and 400 to 600 I.U. of vitamin D daily in food and supplements. Milk products, cooked dark green leafy vegetables, dried beans, canned sardines or salmon and calcium-fortified juices and cereals are rich ...

  4. Calcium

    Science.gov (United States)

    ... in luck if you like sardines and canned salmon with bones. Almond milk. previous continue Working Calcium ... drinks, and cereals. Other Considerations for Building Bones Vitamin D is essential for calcium absorption, so it's ...

  5. Vegetarian diet

    Science.gov (United States)

    ... from these foods: Fatty fish, such as sardines, salmon, and mackerel (this only applies to pescetarians and semi-vegetarians) Egg yolks Foods that are fortified with vitamin D, such as orange juice, cow's milk, soymilk, ...

  6. Meat, Poultry and Fish

    Science.gov (United States)

    ... mackerel, lake trout, herring, sardines, albacore tuna and salmon. Some types of fish may contain high levels ... cholesterol. However, liver is rich in iron and vitamins. A small serving (3 ounces) is OK about ...

  7. Bone Health and Osteoporosis: A Guide for Asian Women Aged 50 and Older

    Science.gov (United States)

    ... calcium-fortified rice calcium-fortified soy milk canned salmon with bones canned sardines with bones milk, yogurt, ... lactase pills, which make milk products more digestible. Vitamin D helps your body absorb calcium. You need ...

  8. Osteomalacia

    Science.gov (United States)

    ... These include foods that are naturally rich in vitamin D, such as oily fish (salmon, mackerel, sardines) and egg yolks. Also look for foods that are fortified with vitamin D, such as cereal, bread, milk and yogurt. ...

  9. make up.contents pg

    African Journals Online (AJOL)

    spamer

    at times of major change between sardine and anchovy regimes ..... Sound in enormous quantities, and during their season, ...... bays, inlets and estuarine areas, being rarely found in ...... available for spawning anchovy (zooplankton biomass.

  10. Pelagic Nekton Distribution - Improving ecosystem-based fisheries management and integrated ecosystem assessments by linking long-term climatic forcing and the Pelagic Nekton Community in the Northern California Current

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Pelagic nekton communities are among the most ecologically and economically important components of marine ecosystems worldwide. From sardines and anchovies to squid...

  11. Download this PDF file

    African Journals Online (AJOL)

    spamer

    which feeds primarily on the large populations of sardine. Sardinops .... 1930 and 1971, with the bulk of the construction completed by .... guanay cormorants were best fitted by the equation ..... Clearly, the population dynamics of seabirds in ...

  12. Download this PDF file

    African Journals Online (AJOL)

    Effect of Neat Sardine Oil with Varies Blends on the Performance and. Emission Characteristics of ... petroleum based fuels have become increasingly important due to ... Rong-ji Li (2009) trasesterified fish oil to produce biodiesel and they ...

  13. NCCLME Ecosystem Indicators - Improving ecosystem-based fisheries management and integrated ecosystem assessments by linking long-term climatic forcing and the Pelagic Nekton Community in the Northern California Current

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Pelagic nekton communities are among the most ecologically and economically important components of marine ecosystems worldwide. From sardines and anchovies to squid...

  14. Antimicrobial and antioxidant activities of cactus polyphenols extract on seafood preservation

    OpenAIRE

    Besbes, Nadia; Joffraud, Jean-Jacques; Ben Khemis, Ines; Amri, Mohamed; Sadok, Saloua

    2016-01-01

    The present work investigated the antimicrobial and antioxidant activities of polyphenols extracted from cactus (Opuntia ficus indica) fruit-peels on sardine fillets during refrigerated storage. Biochemical, microbiological and sensorial indicators of treated sardine fillets; were studied comparatively to control lot. Microbial communities were characterized using phenotyping and molecular identification of bacterial isolates; and culture-independent method (PCR-TTGE) for fingerprinting of ba...

  15. A System for the Preference Evaluation of Cycle Menus

    Science.gov (United States)

    1974-10-01

    Lemon Cookies 5. Sardines, Sea SI oped Potatoes, Peas and Carrots , 4,20 2-IG Pickled Beets and Onion Salad, Marble Cake 4. Meatbali...Bean Salad, Chocolate Chip Cookies 36. Sweet and Scur Pork, Fried Rice, Eggplant, Carrot Raisin & Celery Salad, Ice Cream 37. Barbecued Beef Cubes...Banana Split 99. Sweet & Sour Pork, Baked Potato, Carrots , Jellied Banana Salad, Chocolate Chip Cookies 100. Sardines, Fritters, French Fried Onion

  16. Variación estacional de grandes medusas (Scyphozoa en un sistema de lagunas costeras del sur del Golfo de México (1977/1978

    Directory of Open Access Journals (Sweden)

    Samuel Gomez-Aguirre

    1980-12-01

    Full Text Available Two species of large jelly-fish Aurelia aurita L. and Stomoiophus meleagris Ag, have been registered within a yearly cycle at the system of coastal lagoons "Carmen-Machoria-Redonda ", South of the Gulf of Mexico. A. aurita showed two well defined periods: autumn/winter with predominant young specimens (25 to 100 mm and spring/summer with a wide adult distribution (200 to 400 mm, beeing the juveniles restricted to the internal, regions of the system. S. meleagris gave evidence of adult populations all year round, the greatest population density occurred during the summer/autumn period with maximum sizes of 200 to 300 mm and during the winter/spring period, juveniles from 10 to 30 mm in December and 50 to 100 mm in April, giving a distribution pattern very similar to A. aurita.

  17. Systematics and biogeography of the jellyfish Aurelia labiata (Cnidaria: Scyphozoa).

    Science.gov (United States)

    Gershwin, L A

    2001-08-01

    The hypothesis that the common eastern North Pacific Aurelia is A. aurita is falsified with morphological analysis. The name Aurelia labiata is resurrected, and the species is redescribed, to refer to medusae differing from A. aurita by a suite of characters related to a broad and elongated manubrium. Specifically, the oral arms are short, separated by and arising from the base of the fleshy manubrium, and the planulae are brooded upon the manubrium itself, rather than on the oral arms. Aurelia aurita possesses no corresponding enlarged structure. Furthermore, the number of radial canals is typically much greater in A. labiata, and thus the canals often appear more anastomosed than in A. aurita. Finally, most A. labiata medusae possess a 16-scalloped bell margin, whereas the margin is 8-scalloped in most A. aurita. Separation of the two forms has previously been noted on the basis of allozyme and isozyme analyses and on the histology of the neuromuscular system. Partial 18S rDNA sequencing corroborates these findings. Three distinct morphotypes of A. labiata, corresponding to separate marine bioprovinces, have been identified among 17 populations from San Diego, California, to Prince William Sound, Alaska. The long-undisputed species A. limbata may be simply a color morph of A. labiata, or a species within a yet-unelaborated A. labiata species complex. The first known introduction of Aurelia cf. aurita into southern California waters is documented. Although traditional jellyfish taxonomy tends to recognize many species as cosmopolitan or nearly so, these results indicate that coastal species, such as A. labiata, may experience rapid divergence among isolated populations, and that the taxonomy of such species should therefore be scrutinized with special care.

  18. Complete mitochondrial genome of the moon jellyfish, Aurelia sp. nov. (Cnidaria, Scyphozoa).

    Science.gov (United States)

    Hwang, Dae-Sik; Park, Eunji; Won, Yong-Jin; Lee, Jae-Seong

    2014-02-01

    We sequenced 16,971 bp of the linear mitochondrial DNA of the moon jellyfish Aurelia sp. nov. and characterized it by comparing with Aurelia aurita. They had 13 protein-coding genes (PCGs), 16S rRNA and 12S rRNA with three tRNAs (tRNA-Leu, tRNA-Ser(TGA), tRNA-Met). Both have another two PCGs, orf969 and orf324 with telomeres at both ends. After comparison of Aurelia sp. nov. with Aurelia aurita, we found low-protein similarity of orf969 (59%) and orf324 (75%), respectively, while the other 13 PCGs showed 80% to 98% protein similarities.

  19. Revision of the Platystictidae of the Philippines (Odonata), excluding the Drepanosticta halterata group, with descriptions of twenty-one new species

    NARCIS (Netherlands)

    Tol, van J.

    2005-01-01

    Thirty-one species of the family Platystictidae of the Philippines are revised, i.e. all species recognised, excluding the species of the Drepanosticta halterata-group. The following new taxa are described: 16 species in Drepanosticta Laidlaw: D. acuta spec. nov., D. aurita spec. nov., D. centrosaur

  20. Enkele waarnemingen betreffende de ongeslachtelijke voortplanting van de Scyphozoa:

    NARCIS (Netherlands)

    van Erp, J.

    1958-01-01

    Polyp stages were reared from planulae by keeping adult jellyfish in aquaria. Polyps of the species Aurelia aurita, Cyanea capillata and Chrysaora hysoscella, were kept at different temperatures. Scyphistomae of Aurelia reproduce mainly by stolonization. An influence of temperat

  1. Enkele waarnemingen betreffende de ongeslachtelijke voortplanting van de Scyphozoa:

    NARCIS (Netherlands)

    van Erp, J.

    1958-01-01

    Polyp stages were reared from planulae by keeping adult jellyfish in aquaria. Polyps of the species Aurelia aurita, Cyanea capillata and Chrysaora hysoscella, were kept at different temperatures. Scyphistomae of Aurelia reproduce mainly by stolonization. An influence of

  2. Life cycle assessment of European pilchard (Sardina pilchardus) consumption. A case study for Galicia (NW Spain).

    Science.gov (United States)

    Vázquez-Rowe, Ian; Villanueva-Rey, Pedro; Hospido, Almudena; Moreira, María Teresa; Feijoo, Gumersindo

    2014-03-15

    European pilchard or sardines (Sardina pilchardus) are an attractive raw material to extract from Iberian waters, since they constitute a cheap source of protein and they are a popular product among consumers. This has led to a wide range of final products available for consumers to purchase based on this single raw material. Therefore, this study presents a cross-product environmental assessment using life cycle assessment of three different final products based on sardine landings: canned sardines, fresh sardines and European hake caught by using sardine as bait. In addition, the products were followed throughout their entire life cycle, considering different cooking methods for each final product. Results showed high variability in environmental impacts, not only between the three final products, but also when one single product was cooked in different ways, highlighting the importance that the consumption phase and other post-landing stages may have on the final environmental profile of seafood. Results are then analysed regarding relevant limitations and uncertainties, as well as in terms of the consumer and policy implications. Copyright © 2013 Elsevier B.V. All rights reserved.

  3. Effects of crude oil exposure on bioaccumulation of polycyclic aromatic hydrocarbons and survival of adult and larval stages of gelatinous zooplankton.

    Science.gov (United States)

    Almeda, Rodrigo; Wambaugh, Zoe; Chai, Chao; Wang, Zucheng; Liu, Zhanfei; Buskey, Edward J

    2013-01-01

    Gelatinous zooplankton play an important role in marine food webs both as major consumers of metazooplankton and as prey of apex predators (e.g., tuna, sunfish, sea turtles). However, little is known about the effects of crude oil spills on these important components of planktonic communities. We determined the effects of Louisiana light sweet crude oil exposure on survival and bioaccumulation of polycyclic aromatic hydrocarbons (PAHs) in adult stages of the scyphozoans Pelagia noctiluca and Aurelia aurita and the ctenophore Mnemiopsis leidyi, and on survival of ephyra larvae of A. aurita and cydippid larvae of M. leidyi, in the laboratory. Adult P. noctiluca showed 100% mortality at oil concentration ≥20 µL L(-1) after 16 h. In contrast, low or non-lethal effects were observed on adult stages of A. aurita and M. leidyi exposed at oil concentration ≤25 µL L(-1) after 6 days. Survival of ephyra and cydippid larva decreased with increasing crude oil concentration and exposition time. The median lethal concentration (LC50) for ephyra larvae ranged from 14.41 to 0.15 µL L(-1) after 1 and 3 days, respectively. LC50 for cydippid larvae ranged from 14.52 to 8.94 µL L(-1) after 3 and 6 days, respectively. We observed selective bioaccumulation of chrysene, phenanthrene and pyrene in A. aurita and chrysene, pyrene, benzo[a]pyrene, benzo[b]fluoranthene, benzo[k]fluoranthene, and benzo[a]anthracene in M. leidyi. Overall, our results indicate that (1) A. aurita and M. leidyi adults had a high tolerance to crude oil exposure compared to other zooplankton, whereas P. noctiluca was highly sensitive to crude oil, (2) larval stages of gelatinous zooplankton were more sensitive to crude oil than adult stages, and (3) some of the most toxic PAHs of crude oil can be bioaccumulated in gelatinous zooplankton and potentially be transferred up the food web and contaminate apex predators.

  4. Potential human health risks from toxic metals, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, and organochlorine pesticides via canned fish consumption: estimation of target hazard quotients.

    Science.gov (United States)

    El Morsy, Fadia A M; El-Sadaawy, Manal M; Ahdy, Hoda H H; Abdel-Fattah, Laila M; El-Sikaily, Amany M; Khaled, Azza; Tayel, Fathi M T

    2013-01-01

    Canned fish (tuna and sardine) of different geographical regions were collected randomly from supermarkets and were analyzed for heavy metal contents (Hg, Cd and Pb) polycyclic aromatic hydrocarbons, polychlorinated biphenyls, and pesticides. Canned fish samples (two brands of sardines, six brands of tuna) that were purchased from Egyptian cities between 2009 and 2010, represent four countries (Morocco, Republic of Yemen, Indonesia and Thailand). Health risks on humans via dietary intake of seafood were assessed by the target hazard quotients (THQs), potential non-carcinogenic and carcinogenic effects. The contents of trace metals in canned tuna and canned sardines were found as: 0.149-0.218 μg/g for Cd; 0.312-0.937 μg/g for Pb and 0.053-0.022 μg/g for Hg. Canned sardines fluctuated between 0.55 and 1.82, 1.08 and 1.64, 11.91 and 26.24 ng/g for total HCHs, Total cyclodienes and DDTs respectively while the corresponding concentrations in canned tuna were 0.24-1.85, 0.24-1‥85 and 6.56-49.73 ng/g, respectively. Total PCBs fluctuated between 21.75 and 55.10 for canned sardines and 8.56-208.11 ng/g for canned tuna. On the other hand the total PAHs fluctuated between 0.006-9.775 and 1.556-2.686 ng/g for tuna and sardines. From the human health point of view, there is no adverse health effect for both PAHs and heavy metals content on consumers.

  5. Direct assessment of anchovy by the PELGAS05 acoustic survey

    OpenAIRE

    Masse, Jacques; Beillois, Pierre; Duhamel, Erwan

    2005-01-01

    An acoustic survey was carried out in the bay of Biscay from May 3rd to June 1st on board the French research vessel Thalassa. The objective of PELGAS05 survey was to study the abundance and distribution of pelagic fish in the Bay of Biscay. The target species were mainly anchovy and sardine and were considered in a multi-specific context. The results have to be used during ICES working groups in charge of the assessment of sardine, anchovy, mackerel and horse mackerel and in the frame of t...

  6. Development of canned fish curry

    OpenAIRE

    P. K. Vijayan; Balachandran, K.K.

    1986-01-01

    A process for canning sardine in two different curry media is presented. One curry medium yields a product with medium pungency while the second one has a low pungency. Sardine canned in curry medium having medium pungency developed signs of internal corrosion as well as slight bitter taste at the end of 15 months storage at ambient temperature. Similar phenomenon took place in the other product only at the end of 18 months storage. Being a ready to serve product it needs no further preparati...

  7. A fluid mechanical model for current-generating-feeding jellyfish

    Science.gov (United States)

    Peng, Jifeng; Dabiri, John

    2008-11-01

    Many jellyfish species, e.g. moon jellyfish Aurelia aurita, use body motion to generate fluid currents which carry their prey to the vicinity of their capture appendages. In this study, a model was developed to understand the fluid mechanics for this current-generating-feeding mode of jellyfish. The flow generated by free-swimming Aurelia aurita was measured using digital particle image velocimetry. The dynamics of prey (e.g., brine shrimp Artemia) in the flow field were described by a modified Maxey-Riley equation which takes into consideration the inertia of prey and the escape forces, which prey exert in the presence of predator. A Lagrangian analysis was used to identify the region of the flow in which prey can be captured by the jellyfish and the clearance rate was quantified. The study provides a new methodology to study biological current-generating-feeding and the transport and mixing of particles in fluid flow in general.

  8. Novo transmissor silvestre do "Trypanosoma (Schizotrypanum cruzi" (Chagas, 1909

    Directory of Open Access Journals (Sweden)

    F. Nery Guimarães

    1943-06-01

    Full Text Available In nests of opossum (Didelphis aurita, localized in palm-trees of the species Attalea indaya Dr., the authors found a new tritatoma, the description of which is being made by Dr. H. LENT. They verified that this triatoma had been naturally infected by Trypanosoma (Schizotrhypanum cruzy. Two guinea-pigs were subsequently infected by peritoneal inoculation of excrements of this new triatoma. The xenodiagnosis of these guinea-pigs, made with normal nymphas of. T. megistus and T. infestans resulted positive after 25 days. Evidence was obtained of being the opossum (Didelphis one of the sources infection of the new vector, because several specimens of them were found infected, and also a specimen of D. aurita, which contained trypanosomes with the morphology of T. (S. cruzy in the peripheral blood.

  9. 77 FR 19991 - Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Annual Specifications

    Science.gov (United States)

    2012-04-03

    ... as to minimize the targeting of Pacific sardine. In the event that an incidental set-aside is.... Whether this will occur will depend somewhat on market forces within the fishery, and on the regional... change in the market rate and/or the potential lack of availability of the resource to the fleets...

  10. 75 FR 42610 - Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Closure

    Science.gov (United States)

    2010-07-22

    ... National Oceanic and Atmospheric Administration 50 CFR Part 660 RIN 0648-XX54 Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Closure AGENCY: National Marine Fisheries Service (NMFS... live bait fishery or incidental to other fisheries; the incidental harvest of Pacific sardine...

  11. 76 FR 58720 - Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Closure

    Science.gov (United States)

    2011-09-22

    ... National Oceanic and Atmospheric Administration 50 CFR Part 660 RIN 0648-XA709 Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Closure AGENCY: National Marine Fisheries Service (NMFS... bait fishery or incidental to other fisheries; the incidental harvest of Pacific sardine is limited...

  12. 76 FR 40836 - Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Closure

    Science.gov (United States)

    2011-07-12

    ... National Oceanic and Atmospheric Administration 50 CFR Part 660 RIN 0648-XA554 Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Closure AGENCY: National Marine Fisheries Service (NMFS... live bait fishery or incidental to other fisheries; the incidental harvest of Pacific sardine...

  13. 75 FR 59156 - Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Closure

    Science.gov (United States)

    2010-09-27

    ... National Oceanic and Atmospheric Administration 50 CFR Part 660 RIN 0648-XY79 Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Closure AGENCY: National Marine Fisheries Service (NMFS... bait fishery or incidental to other fisheries; the incidental harvest of Pacific sardine is limited...

  14. 77 FR 50952 - Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Closure

    Science.gov (United States)

    2012-08-23

    ... National Oceanic and Atmospheric Administration 50 CFR Part 660 RIN 0648-XC166 Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Closure AGENCY: National Marine Fisheries Service (NMFS... live bait fishery or incidental to other fisheries; the incidental harvest of Pacific sardine...

  15. 78 FR 51097 - Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Closure

    Science.gov (United States)

    2013-08-20

    ... National Oceanic and Atmospheric Administration 50 CFR Part 660 RIN 0648-XC783 Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Closure AGENCY: National Marine Fisheries Service (NMFS... live bait fishery or incidental to other fisheries; the incidental harvest of Pacific sardine...

  16. JPRS Report, Soviet Union, Kommunist, no. 15, October 1989.

    Science.gov (United States)

    2007-11-02

    prices. Nonetheless, the choice was not bad: beef, mutton, pork , sausages and sardines. This 5-year plan, with every passing year in the oblast the...surmounting the tightening of the capillaries was the main problem in dealing with traumas and many other pathological conditions. This is manifested most

  17. Coastal upwelling ecosystems are known to be parts of the most ...

    African Journals Online (AJOL)

    spamer

    able to sustain extremely large populations of pelagic fish such as sardine ... This major characteristic of coastal upwelling ... Because a coastal upwelling ecosystem is a disper- ..... clupéidés des zones d'upwelling d'Afrique de L'Ouest. Aquat ...

  18. Forage fish, their fisheries and their predators: who drives whom?

    NARCIS (Netherlands)

    Engelhard, G.H.; Peck, M.A.; Rindorf, A.; Smout, S.C.; Deurs, van M.; Raab, K.E.; Andersen, K.H.; Garthe, S.; Lauerburg, R.A.M.; Scott, F.; Brunel, T.P.A.; Aarts, G.M.; Kooten, van T.; Dickey-Collas, M.

    2014-01-01

    The North Sea has a diverse forage fish assemblage, including herring, targeted for human consumption; sandeel, sprat, and Norway pout, exploited by industrial fisheries; and some sardine and anchovy, supporting small-scale fisheries. All show large abundance fluctuations, impacting on fisheries and

  19. Moment-Based Physical Models of Broadband Clutter due to Aggregations of Fish

    Science.gov (United States)

    2013-09-30

    their false alarm rate and latency. Accordingly, a quantitative assesment has begun of the temporal persistence of the observed fish-clutter echoes...Assessment of the Pacific Sardine Resource in 2012 for U.S. Management in 2013,” Pacific Fishery Management Council November 2012 Briefing Book

  20. Nourish Your Brain

    Science.gov (United States)

    ... acids is fatty fish (for example sardines, tuna, salmon, mackerel, and herring). Try to eat this type of fish once or twice a week.Talk to your doctor about the risks and benefits of taking vitamins or supplements. Depending on your overall health and ...

  1. Aging: What to Expect

    Science.gov (United States)

    ... calcium include dairy products, almonds, broccoli, kale, canned salmon with bones, sardines and soy products, such as tofu. If you find it difficult to get enough calcium from your diet, ask your doctor about calcium supplements. Get adequate amounts of vitamin D. For adults ages 19 to 70, the ...

  2. Eating during Pregnancy

    Science.gov (United States)

    ... contraction, nerve function milk, cheese, yogurt, sardines or salmon with bones, spinach Iron red blood cell production (to prevent anemia) lean red meat, spinach, iron-fortified whole-grain breads and cereals Vitamin A healthy skin, good eyesight, growing bones carrots, ...

  3. Vitamin D Deficiency

    Science.gov (United States)

    ... and the foods you eat. Foods with natural vitamin D include: • Certain fish: salmon, sardines, mackerel, tuna • Cod liver oil • Shiitake mushrooms • Egg yolks Foods that often have added vitamin D include: • Dairy products • Orange juice • Infant formula • ...

  4. Egg and larval distributions of seven fish species in north-east Atlantic waters

    NARCIS (Netherlands)

    Ibaibarriaga, L.; Irigoien, X.; Santos, M.; Eltink, A.T.G.W.

    2007-01-01

    The distribution of egg and larvae of mackerel, horse mackerel, sardine, hake, megrim, blue whiting and anchovy along the European Atlantic waters (south Portugal to Scotland) during 1998 is described. Time of the year, sea surface temperature and bottom depth are used to define the spawning habitat

  5. Play and the Avant-Garde: Aren't We All a Little Dada?

    Science.gov (United States)

    Prager, Phillip

    2013-01-01

    Dada, an art movement that became well known in the late 1910s and early 1920s, challenged traditional notions of art and aesthetics. Dada artists, for example, tossed colored scraps of paper into the air to compose chance-based collages, performed sound poems devoid of semantic value, and modeled a headpiece fashioned of sardine cans. To most art…

  6. Treatment of fish processing wastewater in a one-step or two-step upflow anaerobic sludge blanket (UASB) reactor

    NARCIS (Netherlands)

    Paluenzuela-Rollon, A.; Zeeman, G.; Lubberding, H.J.; Lettinga, G.; Alaerts, G.J.

    2002-01-01

    The performance of one-step UASB reactors treating fish processing wastewater of different lipid levels was determined using artificially generated influent simulating that of the canning of sardines and tuna. The organic loading rates (OLR) and the hydraulic retention times (HRT) were 5-8 g COD.l(-

  7. 78 FR 6794 - Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Annual Specifications

    Science.gov (United States)

    2013-01-31

    ... will be accepted in Microsoft Word, Excel, or Adobe PDF file formats only. Copies of the report... biomass estimate from 2012, the result of the HG formula is approximately 40,000 mt less than the 2012 HG... NMFS to set an annual HG for the Pacific sardine fishery based on the harvest formula in the FMP....

  8. Chemical profiles in otoliths from 3 fish species along the Moroccan coast: accumulation and migration patterns

    Directory of Open Access Journals (Sweden)

    Maylis Labonne

    2015-11-01

    First results indicate that the concentrations are highly variable in hake transects, and on the other hand, transects in seabreams seem very stable regardless of location. Transects in sardines indicate different patterns depending on the locations and, some of them, clearly show the upwelling signature.

  9. Mountaineering Away Stress

    Institute of Scientific and Technical Information of China (English)

    Jiang Qinghong

    2011-01-01

    AS a longtime Beijinger,I and other big city dwellers deal with a lot of stress.It comes from everywhere:noise,air pollution,crowded subways where people are packed together like sardines,traffic congestion,rising housing prices and consumer costs,not to mention an ever-growing rich-poor divide.

  10. 76 FR 11969 - Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Annual Specifications

    Science.gov (United States)

    2011-03-04

    ... decrease the future stock of the species, as well as harvest limits, thereby reducing future potential... States; Coastal Pelagic Species Fisheries; Annual Specifications AGENCY: National Marine Fisheries... this species on July 1, 2011. Vessels with Pacific sardine catch must be at shore and in the process...

  11. 78 FR 61840 - Pacific Fishery Management Council (Pacific Council); Public Meetings

    Science.gov (United States)

    2013-10-04

    ... Methodology Review Process and Preliminary Topic Selection 5. Pacific Sardine Stock Assessment and Management... 0648-XC914 Pacific Fishery Management Council (Pacific Council); Public Meetings AGENCY: National... Pacific Council will meet as late as necessary each day to complete its scheduled business. ADDRESSES...

  12. The Benguela ecosystem can be loosely considered to cover the ...

    African Journals Online (AJOL)

    denise

    Changes in the abundance and distribution of commercially important species, as determined by acoustic and trawl surveys and ... Namibian sardine population and many other resources. The present socio-economic value of the Namibian ... Parameter. Benguela ..... colonies (Cape Cross north of Swakopmund, Wolf.

  13. 78 FR 77413 - Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Change to Start of Pacific...

    Science.gov (United States)

    2013-12-23

    ... will remain as specified in the FMP; as would the current quota roll-over provisions. The purpose of... Microsoft Word, Excel, or Adobe PDF file formats only. FOR FURTHER INFORMATION CONTACT: Joshua Lindsay... timing of stock assessments. The process for setting the annual Pacific sardine quotas begins with...

  14. Searching for a stock structure in Sardina pilchardus from the Adriatic and Ionian seas using a microsatellite DNA-based approach

    DEFF Research Database (Denmark)

    Ruggeri, Paolo; Splendiani, Andrea; Bonanomi, Sara;

    2013-01-01

    In the present study the genetic variability of European sardine from Adriatic and Ionian seas was investigated in order to detect the occurrence of genetic structure within and between these basins. In several samples the analysis of genetic variability at eight microsatellite loci showed a numb...

  15. Semantic involvement of initial and final lexical embeddings during sense-making: the advantage of starting late.

    NARCIS (Netherlands)

    van Alphen, P.M.; van Berkum, J.J.A.

    2012-01-01

    During spoken language interpretation, listeners rapidly relate the meaning of each individual word to what has been said before. However, spoken words often contain spurious other words, like 'day' in 'daisy', or 'dean' in 'sardine'. Do listeners also relate the meaning of such unintended, spurious

  16. Treatment of fish processing wastewater in a one-step or two-step upflow anaerobic sludge blanket (UASB) reactor

    NARCIS (Netherlands)

    Paluenzuela-Rollon, A.; Zeeman, G.; Lubberding, H.J.; Lettinga, G.; Alaerts, G.J.

    2002-01-01

    The performance of one-step UASB reactors treating fish processing wastewater of different lipid levels was determined using artificially generated influent simulating that of the canning of sardines and tuna. The organic loading rates (OLR) and the hydraulic retention times (HRT) were 5-8 g

  17. 77 FR 47318 - Fisheries Off West Coast States; Coastal Pelagic Species Fisheries; Annual Specifications

    Science.gov (United States)

    2012-08-08

    ... biomass for Pacific sardine to the Council's CPS Management Team (Team), the Council's CPS Advisory... preventing overfishing and managing the fishery at a level that will achieve optimum yield while allowing all... the FRACTION parameter of the HG rule. Recent work has shown that the strength of the...

  18. 75 FR 59645 - Endangered and Threatened Wildlife and Plants; Determination of Endangered Status for the African...

    Science.gov (United States)

    2010-09-28

    ... populations. The primary concern is preventing the transmission of disease from the large numbers of African... Endangered Species Act of 1973, as amended (Act) (16 U.S.C. 1531 et seq.) is a law that was passed to prevent... horse mackerel has replaced sardines. (5) Comment: One peer reviewer stated that avian cholera...

  19. RECRUITMENT AND RECRUITMENT MODELS IN FISHERY SCIENCE

    Directory of Open Access Journals (Sweden)

    Merica Slišković

    2006-04-01

    Full Text Available Recruitment and growth of individuals bring the most to enlargement of fish stock biomass. Recruitment can be independent on stock size during the development of the fishery, but experience has showed that many fisheries have come to the point when recruitment decreases due to over fishing. Large parental stock does not give large number of juveniles because large number of individuals influences survival of juveniles (competition for food. In period between 1984 and 1987 this phenomenon was recorded in sardine population in the Adriatic Sea, when large biomass of parental stock resulted in twice less recruitment. In the period between 1993 and 2000 there was no significant recruitment of the sardine population in the Adriatic Sea and it reflected on biomass of the sardine. Large recruitment was recorded in 2001 and 2002. There are numerous mathematical models which give relation between stock and recruitment. Beverton–Holt’s and Ricker’s models are usually used. Beverton–Holt’s model assumes that recruitment depends on the density of population. Ricker’s model is based on assumption that all resources are evenly distributed among the individuals; therefore the rise in density causes the exhaustion of resources. This model is suitable for species as the sardine, which migrates in the breeding area.

  20. Lagrangian analysis of fluid transport in empirical vortex ring flows

    OpenAIRE

    Shadden, Shawn C.; Dabiri, John O.; Marsden, Jerrold E.

    2006-01-01

    In this paper we apply dynamical systems analyses and computational tools to fluid transport in empirically measured vortex ring flows. Measurements of quasisteadily propagating vortex rings generated by a mechanical piston-cylinder apparatus reveal lobe dynamics during entrainment and detrainment that are consistent with previous theoretical and numerical studies. In addition, the vortex ring wake of a free-swimming Aurelia aurita jellyfish is measured and analyzed in the framework of dynami...

  1. Cross-species chromosome painting in bats from Madagascar: the contribution of Myzopodidae to revealing ancestral syntenies in Chiroptera.

    Science.gov (United States)

    Richards, Leigh R; Rambau, Ramugondo V; Lamb, Jennifer M; Taylor, Peter J; Yang, Fengtang; Schoeman, M Corrie; Goodman, Steven M

    2010-09-01

    The chiropteran fauna of Madagascar comprises eight of the 19 recognized families of bats, including the endemic Myzopodidae. While recent systematic studies of Malagasy bats have contributed to our understanding of the morphological and genetic diversity of the island's fauna, little is known about their cytosystematics. Here we investigate karyotypic relationships among four species, representing four families of Chiroptera endemic to the Malagasy region using cross-species chromosome painting with painting probes of Myotis myotis: Myzopodidae (Myzopoda aurita, 2n = 26), Molossidae (Mormopterus jugularis, 2n = 48), Miniopteridae (Miniopterus griveaudi, 2n = 46), and Vespertilionidae (Myotis goudoti, 2n = 44). This study represents the first time a member of the family Myzopodidae has been investigated using chromosome painting. Painting probes of M. myotis were used to delimit 29, 24, 23, and 22 homologous chromosomal segments in the genomes of M. aurita, M. jugularis, M. griveaudi, and M. goudoti, respectively. Comparison of GTG-banded homologous chromosomes/chromosomal segments among the four species revealed the genome of M. aurita has been structured through 14 fusions of chromosomes and chromosomal segments of M. myotis chromosomes leading to a karyotype consisting solely of bi-armed chromosomes. In addition, chromosome painting revealed a novel X-autosome translocation in M. aurita. Comparison of our results with published chromosome maps provided further evidence for karyotypic conservatism within the genera Mormopterus, Miniopterus, and Myotis. Mapping of chromosomal rearrangements onto a molecular consensus phylogeny revealed ancestral syntenies shared between Myzopoda and other bat species of the infraorders Pteropodiformes and Vespertilioniformes. Our study provides further evidence for the involvement of Robertsonian (Rb) translocations and fusions/fissions in chromosomal evolution within Chiroptera.

  2. Revision of the Platystictidae of the Philippines (Odonata), excluding the Drepanosticta halterata group, with descriptions of twenty-one new species

    OpenAIRE

    Tol, van, J.

    2005-01-01

    Thirty-one species of the family Platystictidae of the Philippines are revised, i.e. all species recognised, excluding the species of the Drepanosticta halterata-group. The following new taxa are described: 16 species in Drepanosticta Laidlaw: D. acuta spec. nov., D. aurita spec. nov., D. centrosaurus spec. nov., D. clados spec. nov., D. flavomaculata spec. nov., D. furcata spec. nov., D. hermes spec. nov., D. krios spec. nov., D. luzonica spec. nov., D. malleus spec. nov., D. myzouris spec. ...

  3. The neotropical species of Xanthopimpla Saussure (Hymenoptera: Ichneumonidae: Pimplinae).

    Science.gov (United States)

    Gómez, Isrrael C; Sääksjärvi, Ilari E; Broad, Gavin R; Puhakka, Liisa; Castillo, Carol; Peña, Carlos; Pádua, Diego G

    2014-03-04

    Xanthopimpla Saussure, 1892 is one of the largest and best studied genera of the family Ichneumonidae. It is most species rich in the Oriental and Afrotropical regions with only a few species occurring in Central and South America. The present study reviews the Neotropical species of the genus including descriptions of four new species from Amazonia and Northeast South America. We define a new species group: the amazonica species-group, to accommodate the following five species: X. amazonica Gómez, Sääksjärvi & Veijalainen, X. guianensis Gómez & Sääksjärvi sp. n., X. jussilai Veijalainen, Sääksjärvi & Broad, X. pucallpensis Gómez & Sääksjärvi sp. n. and X. vidali Gómez sp. n. The aurita species-group, which had hitherto been regarded as the only species-group in the Neotropical region, is currently represented by five species: X. allpahuaya Gómez & Sääksjärvi sp. n., X. aurita Krieger, X. craspedoptera Krieger, X. rhabdomera Townes and X. spiloptera Krieger. The Andean species X. peruana Krieger is established as an unplaced species outside of the amazonica and aurita species-groups. A key to Neotropical species-groups and species of Xanthopimpla is provided. Xanthopimpla aurita is recorded for the first time from Ecuador and Colombia and its extensive distribution is discussed. Xanthopimpla amazonica, X. craspedoptera and X. jussilai are recorded for the first time from Brazil; X. amazonica is recorded for the first time from French Guiana; X. spiloptera is recorded for the first time from French Guiana and Peru, and X. rhabdomera is recorded for the first time from Peru. 

  4. Invasive Marine and Estuarine Animals of the Pacific Northwest and Alaska

    Science.gov (United States)

    2005-09-01

    Schyphozoan 1 1 Porifera 6 3 1 7 Anthozoan 1 3 1 3 Platyhelminth 2 2 Polychaete 13 16 2 21 Oligochaete 4 3 6 Bivalve 17...Gonothyraea clarki Hydrozoan OR Obelia sp. Hydrozoan WA OR Aurelia aurita Schyphozoan WA Clathria prolifera Porifera WA Clionoa thoosina... Porifera WA Cliona sp. Porifera WA OR AK Halichondria bowerbanki Porifera WA OR Haliclona loosanoffi Porifera OR Haliclona luciae Porifera WA

  5. Invasive Marine and Estuarine Animals of California

    Science.gov (United States)

    2005-08-01

    4 3 5 Porifera 5 5 5 3 7 Polychaete 7 45 13 15 47 Oligochaete 1 4 4 5 Bivalve 16 25 12 6 26 Gastropod 16 23 14 6 26 Nudibranch 2 2 2 2...ballast water, they include species of 13 hydrozoans, 7 sponges ( porifera ), 5 anemones (anthozoans), 3 barnacles, 14 bryozoans, 2 entoprocts, 15...ST SF HU Obelia geniculata Hydrozoan ST Aurelia aurita Scyphozoan NB ST SF HU Phyllorhiza punctatus Scyphozoan NB ST Cliona sp. Porifera

  6. Impact of El Niño events on pelagic fisheries in Peruvian waters

    Science.gov (United States)

    Ñiquen, Miguel; Bouchon, Marilú

    2004-03-01

    Using data from stock assesment surveys on pelagic resources during El Niño events of 1972/73, 1982/83, 1997/98, we analyze biological changes on pelagic ecosystems and pelagic fisheries during different stages of development of El Niño phenomenon: emergence, full, final and post-Niño. Results indicate changes in spatial distribution of resources, their concentration and size structure. In anchovy (Engraulis ringens) a decrease in biomass was observed, which was estimated at 1.2 million tons in September 1998, the lowest throughout the 1990s. This resource showed an asymmetric distribution towards the south of Peru. Other pelagic resources increased their biomass during or after Niño events, primarily sardine (Sardinops sagax), jack mackerel (Trachurus murphyi), pacific mackerel (Scomber japonicus), and longnose anchovy (Anchoa nasus). At the end of the El Niño phenomenon we found less productivity but more diversity in the pelagic ecosystem. During the 1997/98 El Niño, the diversity index (Manual de Ecologia, 1a Edition, Editorial Trillas, Mexico, 267pp) increased from 0.87 to 1.23-1.70. In both the emergence stage and fully developed stages of El Niño we found large numbers of sardine and longnose anchovy present simultaneously. Size structure of sardine, jack mackerel, and pacific mackerel showed an increase in juveniles. Anchovy during El Niño showed a single modal group composed of adults, but the post-Niño phase indicated an increase in juveniles with an average length of 6-7 cm. In El Niño conditions spawning among anchovy was low, but among sardine and pacific mackerel it was high. We observed, for the first time during full spawning, juvenile sardines with a total length of 18-20 cm. The anchovy spawning season during the post-Niño phase was considerably lengthened, from April to December 1998. Drastic change occurred in fisheries when monospecific fisheries, based on anchovy before El Niño, became multispecific fisheries based on sardine, jack

  7. Optimal stroke patterns for a model jellyfish swimmer with thin, flexible body

    Science.gov (United States)

    Peng, Jifeng; Dabiri, John

    2007-11-01

    In this study, a numerical model is built to simulate swimming of oblate jellyfish (e.g. Aurelia aurita). The model swimmer is a thin, axisymmetric circular plate which is flexible and is able to deform, mimicking contraction and relaxation of a jellyfish. Using body deformation, the swimmer is able to swim by shedding vortices into fluid wake. A prescribed body motion extracted from a free-swimming Aurelia aurita is applied to the swimmer. The induced vortex wake is solved by a vortex sheet method and is compared with the wake of the free-swimming Aurelia aurita measured by PIV. The stoke pattern of the swimmer is optimized for minimal cost of locomotion. The body kinematics are parameterized and cost of locomotion is calculated from simulation using the vortex sheet method. A surrogate management framework is used as the optimization scheme. The vortex wake induced by the optimal stroke pattern is investigated to identify the characteristics of the wake which enhance swimming performance.

  8. A biomimetic robotic jellyfish (Robojelly) actuated by shape memory alloy composite actuators.

    Science.gov (United States)

    Villanueva, Alex; Smith, Colin; Priya, Shashank

    2011-09-01

    An analysis is conducted on the design, fabrication and performance of an underwater vehicle mimicking the propulsion mechanism and physical appearance of a medusa (jellyfish). The robotic jellyfish called Robojelly mimics the morphology and kinematics of the Aurelia aurita species. Robojelly actuates using bio-inspired shape memory alloy composite actuators. A systematic fabrication technique was developed to replicate the essential structural features of A. aurita. Robojelly's body was fabricated from RTV silicone having a total mass of 242 g and bell diameter of 164 mm. Robojelly was able to generate enough thrust in static water conditions to propel itself and achieve a proficiency of 0.19 s(-1) while the A. aurita achieves a proficiency of around 0.25 s(-1). A thrust analysis based on empirical measurements for a natural jellyfish was used to compare the performance of the different robotic configurations. The configuration with best performance was a Robojelly with segmented bell and a passive flap structure. Robojelly was found to consume an average power on the order of 17 W with the actuators not having fully reached a thermal steady state.

  9. A biomimetic robotic jellyfish (Robojelly) actuated by shape memory alloy composite actuators

    Energy Technology Data Exchange (ETDEWEB)

    Villanueva, Alex; Smith, Colin; Priya, Shashank, E-mail: spriya@vt.edu [Center for Intelligent Material Systems and Structures (CIMSS), Virginia Tech, Blacksburg, VA 24061 (United States)

    2011-09-15

    An analysis is conducted on the design, fabrication and performance of an underwater vehicle mimicking the propulsion mechanism and physical appearance of a medusa (jellyfish). The robotic jellyfish called Robojelly mimics the morphology and kinematics of the Aurelia aurita species. Robojelly actuates using bio-inspired shape memory alloy composite actuators. A systematic fabrication technique was developed to replicate the essential structural features of A. aurita. Robojelly's body was fabricated from RTV silicone having a total mass of 242 g and bell diameter of 164 mm. Robojelly was able to generate enough thrust in static water conditions to propel itself and achieve a proficiency of 0.19 s{sup -1} while the A. aurita achieves a proficiency of around 0.25 s{sup -1}. A thrust analysis based on empirical measurements for a natural jellyfish was used to compare the performance of the different robotic configurations. The configuration with best performance was a Robojelly with segmented bell and a passive flap structure. Robojelly was found to consume an average power on the order of 17 W with the actuators not having fully reached a thermal steady state.

  10. STUDY ON OCEANGRAFHIC AND WEATHER CONDITIONS RELATED TO THE ABUNDANCE OF SMALL PELAGIC FISHERY IN NATUNA SEA USING REMOTE SENSING DATA

    Directory of Open Access Journals (Sweden)

    Teguh Prayogo

    2012-11-01

    Full Text Available Indonesian waters have abundance of natural resources; the potential of small pelagic fish in Natuna Sea and SouthChina Sea have not been optimized yet explores. Unfortunately, it was caused by lacking in the data of environmentalconditions that have been changed and the information of appropriate fishing ground. Hence, dynamical oceanographicinformation and weather condition is necessary to optimize small pelagic fish exploitation.Research location in Natuna Sea and its surrounding with geographical position is 08°N–03°S; 103°–111°E. Theoceanographic condition representative by monthly SST, Chl-a, SSH that derived from satellite data and Dipole ModeIndex for 2002-2007 from FRCGC website. Monthly wind data is variable for weather condition. Small pelagic fishabundance representative by annual fish production (2002-2005 and monthly Catch Per Unit Effort (CPUE ofGoldstripe sardinella, Bigeye scad and Indian scad (2006. It was data collected from Directorate General of CaptureFisheries (Ministry of Marine Affairs and Fisheries and daily fishing operation (2007 used to calculate match-up ratiothat was collected from Pemangkat fishing port in West Kalimantan. Research process consists of image processing,descriptive correlation analysis and GIS analysis to predict fishing ground map and match-up ratio calculation.Result of this research is the annual fish catch production of Bigeye scad and Indian scad (2002-2005 is tend toincrease and the monthly CPUE of both species is high during SE Monsoon (May-Sep that is condition contrarily in NWMonsoon (Nov-Apr. Meanwhile, the annual fish catch production of Goldstripe sardinella production is tend to decreasefrom 2002-2005, it has CPUE is high in early SE Monsoon (May. During the SE Monsoon (May-Sep when DM Index ispositive (+ the Indian scad and Bigeye scad production is high, for Goldstripe sardinella the fish production is highwhen DM Index is positive (+ in May. The accuracy of prediction map of

  11. New potential host for Ryukyua globosa (Crustacea, Isopoda, Cymothoidae) from Parangipettai, Southeast coast of India.

    Science.gov (United States)

    Rameshkumar, Ganapathy; Ramesh, Mathan; Ravichandran, Samuthirapandian

    2016-12-01

    In the present study Ryukyua globosa parasitic isopod on the marine fishes of the Parangipettai coast are reported. R. globosa was found attached in the gill chamber of Sardinella gibbosa. The attachment position of Ryukyua species differs from that of other member of this genus. Until now, R. globosa was only reported on Amblygaster sirm and A.leiogaster. S.gibbosa is a new host record for R.globosa. R. globosa, branchial parasites, already known from Japan and is now recorded from the Southeast coast of India.

  12. The investigation of morphological characteristics of willow species in different environmental conditions

    Directory of Open Access Journals (Sweden)

    Rodzkin Aleh I.

    2016-01-01

    Full Text Available Alternative or renewable energy is a modern strategy with a good per­spective in the nearest future. There are several directions of renewable energy development that depend on climatic, economic and technological opportunities of a region. The perspective choice for areas with moderate climate is bioenergy. One of the bioenergy directions is agro forestry based on short rotation coppice plantations (SRC of trees, like willow, poplar and others. The goal of experiments was the assessment of the potential of different willow species for the obtaining of energy in two climatic zones and on two types of soils of Belarus. For this purpose several morphological characteristics were metered: height of plants, biomass, diameter and number of sprouts. The field experiments were conducted on two types of soils: post-mining peaty soils in Grodno region and on degraded peaty soils in Brest region of Belarus. The same soils are very problematic for growing of traditional agricultural crops, thus willow production is a good alternative for biomass production of energy as well as for the reclamation of these soils. In our experiments the following species of willow were tested (Salix alba, Salix viminalis, Salix dasyclados, Salix aurita that may grow on peaty soils at the natural conditions. The most popular species for modern selection of SRC of willow is Salix viminalis. Nevertheless, the most suitable morphological characteristics on post-mining peaty soils were established for plants of Salix dasyclados and on degraded peaty soils for the plants of Salix alba. The unfavorable parameters at the both type of soils were identified for the plants of Salix aurita. However, it is necessary to take into account that the used species are more popular for natural wetlands and in our experiments plants have best results of survival of cutting and rates of growth at the beginning of vegetation. In accordance with these facts Salix aurita may not be used for energy

  13. Effects of crude oil exposure on bioaccumulation of polycyclic aromatic hydrocarbons and survival of adult and larval stages of gelatinous zooplankton.

    Directory of Open Access Journals (Sweden)

    Rodrigo Almeda

    Full Text Available Gelatinous zooplankton play an important role in marine food webs both as major consumers of metazooplankton and as prey of apex predators (e.g., tuna, sunfish, sea turtles. However, little is known about the effects of crude oil spills on these important components of planktonic communities. We determined the effects of Louisiana light sweet crude oil exposure on survival and bioaccumulation of polycyclic aromatic hydrocarbons (PAHs in adult stages of the scyphozoans Pelagia noctiluca and Aurelia aurita and the ctenophore Mnemiopsis leidyi, and on survival of ephyra larvae of A. aurita and cydippid larvae of M. leidyi, in the laboratory. Adult P. noctiluca showed 100% mortality at oil concentration ≥20 µL L(-1 after 16 h. In contrast, low or non-lethal effects were observed on adult stages of A. aurita and M. leidyi exposed at oil concentration ≤25 µL L(-1 after 6 days. Survival of ephyra and cydippid larva decreased with increasing crude oil concentration and exposition time. The median lethal concentration (LC50 for ephyra larvae ranged from 14.41 to 0.15 µL L(-1 after 1 and 3 days, respectively. LC50 for cydippid larvae ranged from 14.52 to 8.94 µL L(-1 after 3 and 6 days, respectively. We observed selective bioaccumulation of chrysene, phenanthrene and pyrene in A. aurita and chrysene, pyrene, benzo[a]pyrene, benzo[b]fluoranthene, benzo[k]fluoranthene, and benzo[a]anthracene in M. leidyi. Overall, our results indicate that (1 A. aurita and M. leidyi adults had a high tolerance to crude oil exposure compared to other zooplankton, whereas P. noctiluca was highly sensitive to crude oil, (2 larval stages of gelatinous zooplankton were more sensitive to crude oil than adult stages, and (3 some of the most toxic PAHs of crude oil can be bioaccumulated in gelatinous zooplankton and potentially be transferred up the food web and contaminate apex predators.

  14. Effects of Ocean Climate on Transboundary Movement of Coastal Pelagic Resources Between the EEZs of Mexico and the United States

    Science.gov (United States)

    Baumgartner, T. R.; Garcia, J.; Sanchez, C.; Lo, N. C.; Charter, R.

    2007-05-01

    Interannual to multidecadal changes in ocean climate directly impact access to transboundary coastal pelagic resources between fisheries operating in U.S. and Mexican waters. This study provides a preliminary analysis of the scale of year-to-year shifts in the distribution of the Pacific sardine (Sardinops sagax caeruleus) with data from 2002 and 2003. One of the purposes of this initiative is to provide a template for collaborative research to guide regional policy development for responsible and sustainable utilization of the shared resource. This work is based on coordinated quarterly ocean surveys run by Mexican (the IMECOCAL program=Investigaciones Mexicanas de la Corriente de California) and U.S. scientists (the CalCOFI program=California Cooperative Oceanic Fisheries Investigations) allowing us to evaluate the annual state of the pelagic ecosystem from northern California to southern Baja California. The subject of this study is the "subarctic stock" of the Pacific sardine which is centered off California in the U.S. and extends southwards to the region off central Baja California. Estimates of sardine biomass in U.S. and Mexican waters, based on the rates of egg production measured during the IMECOCAL and CalCOFI surveys of April 2002 and April 2003, show order of magnitude differences in the relative proportions of biomass in the Mexican EEZ that is associated with the contrasts in ocean climate resulting from the regional effects of El Niño during April 2003. Results indicate a significant northward shift of the sardine stock off Mexico during 2003: we estimate that approximately 20 percent of the total biomass of the stock was located in the Mexican EEZ during spring of 2002 while the shift in ocean climate resulted in the presence of only 2 percent of the biomass of the stock in Mexican waters during April, 2003. A second, more southerly sardine stock extended from southern to central Baja California in April, 2003, but it was out of reach of the fleet

  15. KONTRIBUSI INDUSTRI PENGOLAHAN HASIL PERIKANAN TRADISIONAL TERHADAP PENDAPATAN NELAYAN PENGOLAH

    Directory of Open Access Journals (Sweden)

    Ernik Yuliana

    2016-09-01

    Full Text Available Traditional fish processing industries in Muara Angke Jakarta have been involving many labor from the local fishermen or others. This study describes the contributions of many small industry to the labor incomes based on every small industry profit distribution. Survey method had been applied to collect the data. The population are all small traditional fish processing industries in Muara Angke. The samples are chosen based on their product, either salty fish or smoked fish. “Hayami method” was applied to calculate the added value of each small industry. The results of this study shows that salty squid has the highest value added (Rp2167,61/kg and highest profits (Rp2072,61/kg, Indian mackerel smoked fish gives the highest contributions to labor incomes (Rp663,36/kg. Sardinella salty fish gives the highest profit level, followed by salty squid and salty mackerel scad. Salty stingray and salty Indian mackerel gives high value for labor return. In addition, salty sardinella, salty squid, and salty “lesi” fish have high added value ratio. Salty stingray gives the highest other input contribution, followed by smoked Indian mackerel.

  16. Histological Changes in Gills of Two Fish Species as Indicators of Water Quality in Jansen Lagoon (São Luís, Maranhão State, Brazil

    Directory of Open Access Journals (Sweden)

    Débora M. S. Santos

    2014-12-01

    Full Text Available Water quality of the Jansen Lagoon (São Luís, Maranhão State, Brazil was assessed through histological biomarkers and microbiological parameters. To this end, 29 fish specimens (11 Centropomus undecimalis and 18 Sardinella sp and eight water samples were collected during the rainy and dry periods of 2013. The lagoon water showed thermotolerant coliform indices above the limit set forth in CONAMA Resolution 357/2005. Histological changes observed in the gills were: lifting of the respiratory epithelium, hyperplasia of the lamellar epithelium, incomplete and complete fusion of several lamellae, disorganization of the lamellae, congestion of blood vessels, aneurysms, hypertrophy of the respiratory epithelium, hemorrhage and rupture of the lamellar epithelium and parasite. The histological alteration index (HAI average value to Sardinella sp was 31.8 and to C. undecimalis was 22.2. The average HAI value in both species corresponds to category 21–50, with tissue injuries being classified from moderate to severe. The presence of histological injuries and the HAI values indicate that the fish sampled from the Jansen Lagoon are reacting to non-specific xenobiotics present at the site.

  17. Dietary Protected Feed Supplement to Increase Milk Production and Quality of Dairy Cows

    Science.gov (United States)

    Pramono, A.; Handayanta, E.; Widayati, D. T.; Putro, P. P.; Kustono

    2017-04-01

    The efforts to improve and optimize productivity of dairy cows require sufficient availability of nutrients, especially high energy in the early period of lactation. Increasing energy intake in dairy cows can be conducted by increasing the density of energy. The research aimed to evaluate dietary protected feed supplement on milk production and quality, including: fat, protein, and lactose content of Friesian Holstein dairy cow milk. Protected feed supplement was produced from sardine fish oil, through saponification and microencapsulation protection methods. The experiment consists of two treatments i.e. P0: basal diet (control) and P1: basal diet + 3 % protected feed supplement. Each treatment was repeated 15 times. Data were analyzed by independent samples t-test analysis. Results showed that supplementation of protected sardine fish oil had no effect on lactose content, but increased milk yield production (p<0.01), milk fat content (p<0.05), and protein content (p<0.05).

  18. Changes in the northern Benguela ecosystem over three decades: 1970s, 1980s, and 1990s

    DEFF Research Database (Denmark)

    Heymans, J.J.; Shannon, L.J.; Jarre, Astrid

    2004-01-01

    high catches, and had large populations of a few planktivorous fish. In the 1980s, the planktivorous fish species were expanded (horse mackerel, mesopelagic fish, and other small pelagics), although anchovy and sardine biomass was reduced. Catches remained high in the 1980s and the system was well...... connected. In the 1990s, the system was severely stressed, catches were much lower and omnivory was reduced. Most of the energy flowed through few pathways in the 1990s, and the energy was not transferred as efficiently up the trophic chain as in the 1980s. The fishery operated at the highest trophic level...... during the 1980s and there are some indications of "fishing down the foodweb" in this ecosystem between the 1980s and the 1990s. The high catches of sardine and hake in the 1970s are reflected in the high primary production required (PPR) by those compartments; the high catches of horse mackerel...

  19. Prevalence of microbial contamination of fresh seafood product sold in Constantine, Algeria

    Directory of Open Access Journals (Sweden)

    Amira Leila Dib

    2014-12-01

    Full Text Available The seafood products are considered as an important element in the Mediterranean diet and play a significant role in the appearance of diseases and food poisoning. Forty samples of seafood products from various provenances of eastern coast of Algeria were randomly collected from several retail markets at the Constantine region. Total bacterial counts of Aerobic Mesophilic bacteria, Salmonella spp., total and fecal coliforms, Staphylococcus aureus, sulphite reducing anaerobes bacteria, Vibrio spp., were measured. 39 samples (97.5% of the seafood product analysed, were inappropriate for human consumption, solely one sample 2.5% of sardines were suitable for human consumption. Salmonella spp., was detected in one sample (3% of Sardines, Total and fecal coliforms were detected in 39 samples, Clostridium spp. were detected in 5 samples. No strain of Staphylococcus aureus and vibrio were detected. The results of this study constitute an indicator of bacteriological contamination and showed that samples markets were contaminated with potential pathogenic microorganisms.

  20. Physical and Oxidative Stability of Fish Oil-In-Water Emulsions Stabilized with Fish Protein Hydrolysates

    DEFF Research Database (Denmark)

    García Moreno, Pedro Jesús; Guadix, Antonio; Guadix, Emilia M.

    2016-01-01

    The emulsifying and antioxidant properties of fish protein hydrolysates (FPH) for the physical and oxidative stabilization of 5% (by weight) fish oil-in-water emulsions were investigated. Muscle proteins from sardine (Sardina pilchardus) and small-spotted catshark (Scyliorhinus canicula) were......% yielded a physically stable emulsion with low concentration of unsaturated aldehydes. These results show the potential of FPH as alternative protein emulsifiers for the production of oxidatively stable fish oil-in-water emulsions....... hydrolyzed to degrees of hydrolysis (DH) of 3-4-5-6% with subtilisin. Sardine hydrolysates with low DH, 3% and 4%, presented the most effective peptides to physically stabilize emulsions with smaller droplet size. This implied more protein adsorbed at the interface to act as physical barrier against...

  1. Direct assessment of anchovy by the Pelgas06 acoustic survey

    OpenAIRE

    Masse, Jacques; Mehault, Sonia; Beillois, Pierre; Duhamel, Erwan; Planque, Benjamin; Petitgas, Pierre (collab.); Biseau, Alain

    2006-01-01

    Une campagne acoustique a été organisée dans le golfe de Gascogne du 1er au 30 mai, à bord du navire océanographique français, Thalassa. L'objectif de cette campagne Pelgas 06 était d'étudier l'abondance et la distribution des poissons pélagiques dans le golfe de Gascogne. Les deux espèces cibles étaient principalement l'anchois et la sardine. Les résultats ont été utilisés lors des groupes de travail en charge de l'estimation des stocks de sardine, anchois, maquereau et chinchard et dans le ...

  2. Direct assessment of anchovy by the PELGAS04 acoustic survey

    OpenAIRE

    Masse, Jacques; Beillois, Pierre; Duhamel, Erwan

    2004-01-01

    Une campagne acoustique a été organisée dans le golfe de Gascogne du 27 avril au 25 mai 2004, à bord du navire océanographique français, Thalassa. L'objectif de cette campagne Pelgas 06 était d'étudier l'abondance et la distribution des poissons pélagiques dans le golfe de Gascogne. Les deux espèces cibles étaient principalement l'anchois et la sardine. Les résultats ont été utilisés lors des groupes de travail en charge de l'estimation des stocks de sardine, anchois, maquereau et chinchard e...

  3. Effects of dietary calcium on Helicobacter pylori-induced gastritis in Mongolian gerbils.

    Science.gov (United States)

    Iimuro, Masaki; Nakamura, Shiro; Arakawa, Tetsuo; Wakabayashi, Keiji; Mutoh, Michihiro

    2013-09-01

    Helicobacter pylori (Hp) infection causes gastritis and is considered a gastric cancer risk factor. We have previously reported that codfish meal markedly enhanced Hp-induced gastritis in Mongolian gerbils. In the present study, we sought the responsible components in codfish meal. Codfish were divided into three parts (meat, viscera and 'other parts', including bone), and administered to Hp-infected gerbils. Subsequently, cod bone, sardine bone and prawn shell were tested, along with major calcium components, hydroxyapatite and calcium carbonate, in bone and shell, respectively. 'Other parts' and cod bone enhanced Hp-induced gastritis, as was observed for whole codfish. Similarly, sardine bone and prawn shell, as well as 0.22-0.88% hydroxyapatite and calcium carbonate, enhanced gastritis. In contrast, administration of a higher dose of the calcium compounds exerted protective effects. Intake of calcium compounds may contribute to enhancement of Hp-induced gastritis.

  4. [The content of biogenic amines in canned fish from the Turkish market].

    Science.gov (United States)

    Erkan, N; Helle, N; Ozden, O

    2001-01-01

    This paper describes the determination of biogenic amines in a number of canned fish of the Turkish market by means of HPLC. Over all 39 cans of sardine, tuna, sardel, mackerel, pelamide and trout were tested. The determination of agmatine, cadaverine, histamine, putrescine and tyramine was performed by HPLC with post column derivatization with ortho-phthaldialdehyde. Four samples contained higher amounts of biogenic amines (three sardine samples contained more than 1000 mg/kg histamine) while all other samples were acceptable. These results demonstrate the need of instrumental analyses in the quality control system of Turkish producers of canned fish. The quality of the products at least of some producers has to be improved, e.g. by cooperation between the official food control and the quality assurance system of the producers.

  5. Concentrates of DHA from fish oil by selective esterification of cholesterol by immobilized isoforms of lipase from Candida rugosa.

    Science.gov (United States)

    Jonzo; Hiol; Zagol; Druet; Comeau

    2000-09-01

    Two lipases (Lip A and Lip B), were purified from a commercial lipase preparation produced by Candida rugosa and partially characterized. The purified lipases were immobilized on Duolite A 568 and used in the selective esterification of cholesterol with free fatty acids from sardine fish oil. The results showed that Lip A and Lip B preferentially esterified saturated and monounsaturated fatty acids allowing a 3.4-fold (Lip B, 24 h) and 4-fold (Lip A, 10 h) enrichment of docosahexaenoic acid in the remaining free fatty acid fraction. Selectivity towards eicosapentaenoic acid was less pronounced. By this selective esterification docosahexaenoic acid was concentrated from 7.4 to 32% with a recovery of 95% of its initial content in sardine fish oil.

  6. How sailfish use their bills to capture schooling prey

    DEFF Research Database (Denmark)

    Domenici, P.; Wilson, A.D.M.; Kurvers, R.H.J.M.

    2014-01-01

    The istiophorid family of billfishes is characterized by an extended rostrum or ‘bill’. While various functions (e.g. foraging and hydrodynamic benefits) have been proposed for this structure, until now no study has directly investigated the mechanisms by which billfishes use their rostrum to feed...... on prey. Here, we present the first unequivocal evidence of how the bill is used by Atlantic sailfish (Istiophorus albicans) to attack schooling sardines in the open ocean. Using high-speed video-analysis, we show that (i) sailfish manage to insert their bill into sardine schools without eliciting...... an evasive response and (ii) subsequently use their bill to either tap on individual prey targets or to slash through the school with powerful lateral motions characterized by one of the highest accelerations ever recorded in an aquatic vertebrate. Our results demonstrate that the combination of stealth...

  7. [WTO Case Review Series No. 5] United States—Measures Affecting the Production and Sale of Clove Cigarettes (DS406):The Relationship between Article 2.1 of TBT Agreement and GATT Article III:4 (Japanese)

    OpenAIRE

    NAIKI Yoshiko

    2013-01-01

    There have been very few World Trade Organization (WTO) dispute settlement cases based on the Agreement of the Technical Barriers to Trade (TBT Agreement). While the rulings of the European Communities-Measures Affecting Asbestos and Products Containing Asbestos and European Communities-Trade Description of Sardines are well-known, there were no rulings which addressed the core obligations of the TBT Agreement, namely Articles 2.1 and 2.2. The case of United States - Measures Affecting the Pr...

  8. Characteristics of Fishes with the Potential to Cause Acoustic Clutter off Oregon and Washington during the Summer of 2012

    Science.gov (United States)

    2014-05-10

    Oregon and Washington, the Mexican catch will not be considered further. Figure 3c shows catches of Pacific sardine since 2000 for the other five...its side-aspect target strength is about -31 dB. ACKNOWLEDGMENTS The author greatly appreciates the assistance of Dr. Kelly Benoit- Bird , Dr...Assessment Report 2, Appendix B, Pacific Fishery Management Council, Portland, OR, 2012. 6. Benoit- Bird , K., OSU, Personal communications. 7. Hill, K. T

  9. [Effect of different baits as attractant for blowflies (Diptera) at Valonguinho, Universidade Federal Fluminense, Niterói, RJ, Brazil].

    Science.gov (United States)

    d'Almeida, José M; Fraga, Mariana B

    2007-01-01

    It was carried out a survey of blowflies in an area of the Campus (Valonguinho) of the Universidade Federal Fluminense, Niterói, Rio de Janeiro. The collections were performed with traps, using baits of fish (sardine), bovine liver, shrimps and banana. Were collected 6015 flies, Chrysomya megacephala and Lucilia eximia were the most frequent (50.55% and 21.52%, respectively). The flies were more abundant in February and March and the most attractive bait was fish (38.32%).

  10. Predicting Trophic Interactions and Habitat Utilization in the California Current Ecosystem

    Science.gov (United States)

    2015-09-30

    assimilation (A. Moore), forage fish ecology (K. Rose ) and pinniped ecology (D. Costa). The team also includes a postdoctoral research associate (L...task (1) led to the submission of two manuscripts to Progress in Oceanography describing the fully coupled ecosystem model framework ( Rose et al., 2015...right: spatial patterns and percent variance explained. Center: normalized amplitudes (red squares = sea lion; blue triangles = sardine). Figure

  11. Near East/South Asia Report: No. 2741

    Science.gov (United States)

    1983-04-21

    with the blood of the sons of Chad, Libya, and Algeria until 1904 when the French triumphed in this war against the insurgents." During one of my...PLO. This was after the Palestinian National Council had met in Algeria and issued resolutions and recommendations. President Mubarak reiterated his...all kinds, includ- ing sardines, crustaceans , shellfish and other kinds. 2. Study the natural environment and fighting pollution. 3. Improving and

  12. Synthesis of structured triacylglycerols enriched in n-3 fatty acids by immobilized microbial lipase.

    Science.gov (United States)

    Araújo, Maria Elisa Melo Branco de; Campos, Paula Renata Bueno; Alberto, Thiago Grando; Contesini, Fabiano Jares; Carvalho, Patrícia de Oliveira

    The search for new biocatalysts has aroused great interest due to the variety of micro-organisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sardine oil in solvent-free media. For the immobilization process, the best lipase/support ratios were 1:3 (w/w) for Aspergillus niger lipase and 1:5 (w/w) for Rhizopus javanicus lipase using Amberlite MB-1. Both lipases maintained constant activity for 6 months at 4°C. Reaction time, sardine-free fatty acids:soybean oil mole ratio and initial water content of the lipase were investigated to determine their effects on n-3 long-chain polyunsaturated fatty acids incorporation into soybean oil. Structured triacylglycerols with 11.7 and 7.2% of eicosapentaenoic acid+docosahexaenoic acid were obtained using Aspergillus niger lipase and Rhizopus javanicus lipase, decreasing the n-6/n-3 fatty acids ratio of soybean oil (11:1 to 3.5:1 and 4.7:1, respectively). The best reaction conditions were: initial water content of lipase of 0.86% (w/w), sardine-free faty acids:soybean oil mole ratio of 3:1 and reaction time of 36h, at 40°C. The significant factors for the acidolysis reaction were the sardine-free fatty acids:soybean oil mole ratio and reaction time. The characterization of structured triacylglycerols was obtained using easy ambient sonic-spray ionization mass spectrometry. The enzymatic reaction led to the formation of many structured triacylglycerols containing eicosapentaenoic acid, docosahexaenoic acid or both polyunsaturated fatty acids. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  13. Musculoskeletal Health and Injury Prevention

    Science.gov (United States)

    2008-07-01

    and vegetables: 4 - 5 of each/day; • Eat cold water fish ( salmon , halibut, scallops,tuna, mackerel,cod, shrimp, snapper, and sardines) ≥ 2x...Oats • Soy • Brown rice • Wheat • Cold-water fish Dietary Supplements • Glucosamine/Chondroitin sulfate • Vitamins C and E • Selenium...Omega-3 Fatty Acids • Calcium and Vitamin D • Coenzyme Q10 • Capsaicin Cream (chili peppers) Summary Musculoskeletal health requires: • A

  14. Bio-Alpha off the West Coast

    Science.gov (United States)

    2012-09-01

    Barbara Channel ( SBC ) (Diachok, 2005a), particularly at low frequencies. To isolate the effects of the bottom on TL at low frequencies, we developed a...remain in schools at shallow depths at night (Diachok, 1999). The percentage of sardines in schools at night in the SBC may be anomalously high due...directed at hake, and no hake were caught, but they may have been present in large numbers in the SBC during this experiment. We expect to measure

  15. Different Levels of Hypoxia Tolerance during Early Life History Stages of Key Fish Species from the Northern Benguela Upwelling Ecosystem Inferred from the Comparison of Eco-Physiological Traits

    Science.gov (United States)

    Geist, S. J.; Imam, R. M.; Kunzmann, A.; Ekau, W.

    2016-02-01

    Global change factors such as a pronounced Oxygen Minimum Zone and the shoaling of hypoxic waters are assumed to play a major role in controlling the recruitment of fish stocks in Upwelling Systems by affecting the planktonic early life history stages. Ecological and ecophysiological traits in the larval stages of five key fish species in the Northern Benguela Upwelling System (Sardine, Sardinops sagax; Anchovy, Engraulis encrasicolus; Cape horse mackerel, Trachurus capensis; Cape hake, Merluccius sp.; Pelagic goby, Sufflogobobius bibarbatus) were investigated during the GENUS (Geochemistry and Ecology of the Namibian Upwelling Ecosystem) research project . Analysis of vertical larval distributions in relation to the depth of hypoxic water layers showed gradual interspecific differences, suggesting lower hypoxia tolerance levels of the small pelagics Sardine and Anchovy. Cape horse mackerel juveniles and larvae exhibited very high tolerance levels to short-term hypoxia in respirometry stress experiments, close to the levels of the extremely hypoxia-tolerant Pelagic goby. In the latter two species, we also measured the highest activities of anaerobic enzymes (pyruvate kinase and lactate dehydrogenase) in early and late larval stages, compared to very low activities in Sardine larvae. A higher amount of anaerobic enzymatic activity is related to a higher capacity to break down metabolites that build up during phases of oxygen debt and thus help the larvae to quickly recover from hypoxia exposure. In consequence, a high hypoxia tolerance during their early life stages allows Cape horse mackerel and Pelagic goby to successfully reproduce in an environment characterized by frequent hypoxic events. The low hypoxia tolerance of Sardine larvae, eventually resulting in higher mortality rates, is likely to be an important factor to understand the poor reproductive success and continuing recruitment failures of this formerly dominant fish species of the NBUS during the last

  16. Oxygen: a fundamental property regulating pelagic ecosystem structure in the coastal southeastern tropical Pacific.

    Directory of Open Access Journals (Sweden)

    Arnaud Bertrand

    Full Text Available BACKGROUND: In the southeastern tropical Pacific anchovy (Engraulis ringens and sardine (Sardinops sagax abundance have recently fluctuated on multidecadal scales and food and temperature have been proposed as the key parameters explaining these changes. However, ecological and paleoecological studies, and the fact that anchovies and sardines are favored differently in other regions, raise questions about the role of temperature. Here we investigate the role of oxygen in structuring fish populations in the Peruvian upwelling ecosystem that has evolved over anoxic conditions and is one of the world's most productive ecosystems in terms of forage fish. This study is particularly relevant given that the distribution of oxygen in the ocean is changing with uncertain consequences. METHODOLOGY/PRINCIPAL FINDINGS: A comprehensive data set is used to show how oxygen concentration and oxycline depth affect the abundance and distribution of pelagic fish. We show that the effects of oxygen on anchovy and sardine are opposite. Anchovy flourishes under relatively low oxygen conditions while sardine avoid periods/areas with low oxygen concentration and restricted habitat. Oxygen consumption, trophic structure and habitat compression play a fundamental role in fish dynamics in this important ecosystem. CONCLUSIONS/SIGNIFICANCE: For the ocean off Peru we suggest that a key process, the need to breathe, has been neglected previously. Inclusion of this missing piece allows the development of a comprehensive conceptual model of pelagic fish populations and change in an ocean ecosystem impacted by low oxygen. Should current trends in oxygen in the ocean continue similar effects may be evident in other coastal upwelling ecosystems.

  17. Synthesis of structured triacylglycerols enriched in n-3 fatty acids by immobilized microbial lipase

    Directory of Open Access Journals (Sweden)

    Maria Elisa Melo Branco de Araújo

    Full Text Available Abstract The search for new biocatalysts has aroused great interest due to the variety of micro-organisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sardine oil in solvent-free media. For the immobilization process, the best lipase/support ratios were 1:3 (w/w for Aspergillus niger lipase and 1:5 (w/w for Rhizopus javanicus lipase using Amberlite MB-1. Both lipases maintained constant activity for 6 months at 4 °C. Reaction time, sardine-free fatty acids:soybean oil mole ratio and initial water content of the lipase were investigated to determine their effects on n-3 long-chain polyunsaturated fatty acids incorporation into soybean oil. Structured triacylglycerols with 11.7 and 7.2% of eicosapentaenoic acid + docosahexaenoic acid were obtained using Aspergillus niger lipase and Rhizopus javanicus lipase, decreasing the n-6/n-3 fatty acids ratio of soybean oil (11:1 to 3.5:1 and 4.7:1, respectively. The best reaction conditions were: initial water content of lipase of 0.86% (w/w, sardine-free faty acids:soybean oil mole ratio of 3:1 and reaction time of 36 h, at 40 °C. The significant factors for the acidolysis reaction were the sardine-free fatty acids:soybean oil mole ratio and reaction time. The characterization of structured triacylglycerols was obtained using easy ambient sonic-spray ionization mass spectrometry. The enzymatic reaction led to the formation of many structured triacylglycerols containing eicosapentaenoic acid, docosahexaenoic acid or both polyunsaturated fatty acids.

  18. Annual report 1995

    OpenAIRE

    1995-01-01

    An account is given of the activities of the Institute during the year 1995, including also reports of various projects carried out by staff members, which concerned the following: pre-recruitment ecology of the freshwater sardine (Limnothrissa miodon) in Lake Kariba (Zimbabwe); hydroacoustic surveys of kapenta abundance in Lake Kariba and Lake Cahora Bassa (Mozambique); angler's tigerfish catches, tigerfish studies and gillnet sampling; inshore fish population studies in Lake Kariba; catch/e...

  19. 胶州湾海月水母(Aurelia sp.1)丰度周年变化及对浮游动物群落的影响%ANNUAL OCCURRENCE OF MOON JELLYFISH A URELIA sp.1 IN THE JIAOZHOU BAY AND ITS IMPACTS ON ZOOPLANKTON COMMUNITY

    Institute of Scientific and Technical Information of China (English)

    万艾勇; 张光涛

    2012-01-01

    2009年2—12月间在胶州湾大沽河口邻近海域的逐月现场调查中,利用垂直拖网研究了海月水母碟状体和水母体的时空分布情况以及对其它浮游动物类群的影响,并探讨了海月水母的生态适应性。结果表明:海月水母的碟状体4月份开始在胶州湾出现,并且丰度逐渐增加,到6月份达到最高峰。海月水母的水母体集中在7月份大量出现。碟状体和水母体高峰期的月平均丰度分别为2.9和1.3ind/m2。碟状体开始出现时的水温平均为11.5℃,数量高峰期的水温为20.6℃,而水母体高峰期的水温为25.2℃。该海域的浮游动物总丰度(不含夜光虫)在5—7月份有个高峰期,其中5月份最高值为486.9ind/m2。7月份海月水母高峰期,浮游动物的丰度没有明显下降,两者丰度的地理变化之间也没有显著的相关性。但是通过对2006--2010年间的浮游动物各类群丰度对比,2009年海月水母暴发时夜光虫和桡足类春季丰度高值显著低于其它年份。%During February--December, 2009, monthly investigations were carried out in north-western part of the Jiaozhou Bay. Abundances of the moon jellyfish Aurelia aurita and other zooplankton groups were investigated with ver- tical net towing. Ecological adaption and impacts on other zooplankton groups of moon jellyfish were analysed, and com- pared with synchronous zooplankton and environmental data. Ephyra ofA. aurita was observed from April to June, when its abundance increased from 0.1 to 2.9ind/m3. Medusa ofA. aurita was captured only in July with an average abundance of 1.3ind/m3. The average water temperatures were 11.5 ~C at occurrence of ephyra and 20.6 and 25.2~C during abundance peak of ephyra and medusae, respectively. Total zooplankton abundance (without A. aurita and Noctiluca seintillans) was high from May to July, with the highest of 486.9ind/m3 being observed in May. In July, no

  20. Attractors and soak times in artisanal fi shing with traps

    Directory of Open Access Journals (Sweden)

    Evandro Figueiredo Sebastiani

    2009-12-01

    Full Text Available Traps are used by artisanal fishers as fishing gear in places where other fishing modalities are impeded or limited. The advantage of this type of fishing modality is the possibility of keeping fish alive and in the case of capturing species of low commercial value or size below the permitted minimum this fishing gear allows the release of such specimens back to nature, resulting in a sustainability aspect to the use of this fishing gear. This study aims to evaluate the effects of different attractors and times of submersion on the efficiency of the traps used. Sardines, shrimps and trash fish were employed as attractors. To evaluate the soak time, two periods were tested: 24 and 96 hours. The sardines, used as the attractor, resulted in a production of 1,296.4 ± 397.4g, significantly superior (p <0.05 to other attractors. In relation to the soak time, the period of 24 hours resulted in an average production of 1,719.2 ± 866.0g, significantly (p <0.05 superior to the period of 96 hours. The results led to the conclusion that to optimize this capture by fishing gear, sardines should be used as the attractor, together with a soak time of 24 hours.

  1. Thresher sharks use tail-slaps as a hunting strategy.

    Science.gov (United States)

    Oliver, Simon P; Turner, John R; Gann, Klemens; Silvosa, Medel; D'Urban Jackson, Tim

    2013-01-01

    The hunting strategies of pelagic thresher sharks (Alopias pelagicus) were investigated at Pescador Island in the Philippines. It has long been suspected that thresher sharks hunt with their scythe-like tails but the kinematics associated with the behaviour in the wild are poorly understood. From 61 observations recorded by handheld underwater video camera between June and October 2010, 25 thresher shark shunting events were analysed. Thresher sharks employed tail-slaps to debilitate sardines at all times of day. Hunting events comprised preparation, strike, wind-down recovery and prey item collection phases, which occurred sequentially. Preparation phases were significantly longer than the others, presumably to enable a shark to windup a tail-slap. Tail-slaps were initiated by an adduction of the pectoral fins, a manoeuvre that changed a thresher shark's pitch promoting its posterior region to lift rapidly, and stall its approach. Tail-slaps occurred with such force that they may have caused dissolved gas to diffuse out of the water column forming bubbles. Thresher sharks were able to consume more than one sardine at a time, suggesting that tail-slapping is an effective foraging strategy for hunting schooling prey. Pelagic thresher sharks appear to pursue sardines opportunistically by day and night, which may make them vulnerable to fisheries. Alopiids possess specialist pectoral and caudal fins that are likely to have evolved, at least in part, for tail-slapping. The evidence is now clear; thresher sharks really do hunt with their tails.

  2. Fish Product-Borne Histamine Intoxication Outbreak and Survey of Imported Fish and Fish Products in Serbia.

    Science.gov (United States)

    Petrovic, Jelena; Babić, Jelena; Jaksic, Sandra; Kartalovic, Brankica; Ljubojevic, Dragana; Cirkovic, Miroslav

    2016-01-01

    Histamine levels in fish and fish products are regarded as a primary criterion for food safety, considering the effects of histamine on human health. The aim of this research was to describe a case of massive histamine intoxication in Serbian children and to provide relevant information regarding the presence of histamine in imported marine fish and fish products available in the Serbian market. In January 2014, an incident of foodborne illness occurred in 28 children (aged 2 to 5 years) who consumed canned sardines in a kindergarten in Vojvodina province, northern Serbia. The diagnosis was established based on anamnestic data, epidemiological data, and clinical symptoms and confirmed by positive histamine finding in the incriminated sardines. Substantially high histamine levels (>300 mg/kg) were detected in seven of the nine examined units of these canned sardines. In addition, during an official veterinary border control from January 2013 to January 2015, 273 lots in total, including 2,457 fish and fishery product units, were analyzed using enzyme-linked immunosorbent assay. Even though only nine (3.29%) of all examined lots were declared as unsafe for human consumption, the presented case of histamine intoxication strongly suggests the importance of border inspection and comprehensive control of each imported lot.

  3. A recent increase of swift terns Thalasseus bergii off South Africa - The possible influence of an altered abundance and distribution of prey

    Science.gov (United States)

    Crawford, Robert J. M.

    2009-12-01

    In the 2000s, there were large increases in the numbers of swift terns Thalasseus bergii breeding in the Western and Eastern Cape Provinces of South Africa, which are most plausibly attributed to good recruitment and to an increase in the proportion of mature birds breeding. Numbers increased coincidentally with a greatly increased abundance of two of the main prey species of swift terns, sardine Sardinops sagax and anchovy Engraulis encrasicolus, and remained high as these resources decreased. After 2005, numbers of swift terns breeding in the north and central portions of the Western Cape decreased, whereas numbers breeding farther south in that province increased. This followed displacements to the south and east of sardine and anchovy. In southern Africa, swift terns show low fidelity to breeding localities, which enables a rapid adjustment of the location of breeding to an altered availability of prey. For two seabirds that feed mainly on sardine and anchovy, but once breeding show high fidelity to colonies, African penguin Spheniscus demersus and Cape gannet Morus capensis, proportions breeding in the south and east also increased, but there were substantial decreases in overall numbers breeding in the Western Cape.

  4. The influence of finfish aquaculture on benthic fish and crustacean assemblages in Fitzgerald Bay, South Australia.

    Science.gov (United States)

    Tanner, Jason E; Williams, Kane

    2015-01-01

    The influence of sea-cage aquaculture on wildfish assemblages has received little attention outside of Europe. Sea-cage aquaculture of finfish is a major focus in South Australia, and while the main species farmed is southern bluefin tuna (Thunnus maccoyii), there is also an important yellowtail kingfish (Seriola lalandi) industry. Yellowtail kingfish aquaculture did not appear to have any local or regional effects on demersal assemblages (primarily fish, but also some crustaceans) surveyed by baited remote underwater video (BRUV) in Fitzgerald Bay. We did, however, detect small scale spatial variations in assemblages within the bay. The type of bait used strongly influenced the assemblage recorded, with significantly greater numbers of fish attracted to deployments where sardines were used as the bait to compared to those with no bait. The pelleted feed used by the aquaculture industry was just as attractive as sardines at one site, and intermediate between sardines and no bait at the other. There was significant temporal variability in assemblages at both farm sites and one control site, while the second control site was temporally stable (over the 9 weeks of the study). Overall, the results suggested that aquaculture was having little if any impact on the abundance and assemblage structure of the demersal macrofauna in Fitzgerald Bay.

  5. Pelagic resources landings in central-southern Chile under the A2 climate change scenarios

    Science.gov (United States)

    Yáñez, Eleuterio; Plaza, Francisco; Silva, Claudio; Sánchez, Felipe; Barbieri, María Ángela; Aranis, Antonio

    2016-10-01

    Artificial neural networks (ANNs) were used to predict landings of anchovy ( Engraulis ringens), common sardine ( Strangomera bentincki), and jack mackerel ( Trachurus murphyi) in central-southern Chile. Twelve environmental variables were considered along with fishing effort (fe) and landing statistics from 1973 to 2012. During external validation, the best models with all of the selected variables gave r 2 values of 90 % for anchovy, 96 % for common sardine, and 88 % for jack mackerel. The models were simplified by considering only fe and sea surface temperature from NCEP/NCAR reanalysis data (SST-NOAA), and very similar fits were achieved (87, 92, and 88 %, respectively). Future SSTs were obtained from the A2 climate change scenario and regionalized using statistical downscaling techniques. The downscaled SSTs were used as input for landings predictions using ANN simplified models. In addition, three scenarios of future fishing efforts (2010-2012 average, average + 50 %, and average - 50 %) were used as the input data for landing simulations. The results of the predictions show a decrease of 9 % in future landings of sardine and an increase of 17 % for jack mackerel when comparing 2015 and 2065 monthly projections. However, no significant differences are shown when comparing the estimated landings for the three fishing effort scenarios. Finally, more integrative and complex conceptual models that consider oceanographic-biophysical, physiological, environmental-resource, and interspecies processes need to be implemented.

  6. Thresher sharks use tail-slaps as a hunting strategy.

    Directory of Open Access Journals (Sweden)

    Simon P Oliver

    Full Text Available The hunting strategies of pelagic thresher sharks (Alopias pelagicus were investigated at Pescador Island in the Philippines. It has long been suspected that thresher sharks hunt with their scythe-like tails but the kinematics associated with the behaviour in the wild are poorly understood. From 61 observations recorded by handheld underwater video camera between June and October 2010, 25 thresher shark shunting events were analysed. Thresher sharks employed tail-slaps to debilitate sardines at all times of day. Hunting events comprised preparation, strike, wind-down recovery and prey item collection phases, which occurred sequentially. Preparation phases were significantly longer than the others, presumably to enable a shark to windup a tail-slap. Tail-slaps were initiated by an adduction of the pectoral fins, a manoeuvre that changed a thresher shark's pitch promoting its posterior region to lift rapidly, and stall its approach. Tail-slaps occurred with such force that they may have caused dissolved gas to diffuse out of the water column forming bubbles. Thresher sharks were able to consume more than one sardine at a time, suggesting that tail-slapping is an effective foraging strategy for hunting schooling prey. Pelagic thresher sharks appear to pursue sardines opportunistically by day and night, which may make them vulnerable to fisheries. Alopiids possess specialist pectoral and caudal fins that are likely to have evolved, at least in part, for tail-slapping. The evidence is now clear; thresher sharks really do hunt with their tails.

  7. Not all jellyfish are equal: isotopic evidence for inter- and intraspecific variation in jellyfish trophic ecology

    Directory of Open Access Journals (Sweden)

    Nicholas E.C. Fleming

    2015-07-01

    Full Text Available Jellyfish are highly topical within studies of pelagic food-webs and there is a growing realisation that their role is more complex than once thought. Efforts being made to include jellyfish within fisheries and ecosystem models are an important step forward, but our present understanding of their underlying trophic ecology can lead to their oversimplification in these models. Gelatinous zooplankton represent a polyphyletic assemblage spanning >2,000 species that inhabit coastal seas to the deep-ocean and employ a wide variety of foraging strategies. Despite this diversity, many contemporary modelling approaches include jellyfish as a single functional group feeding at one or two trophic levels at most. Recent reviews have drawn attention to this issue and highlighted the need for improved communication between biologists and theoreticians if this problem is to be overcome. We used stable isotopes to investigate the trophic ecology of three co-occurring scyphozoan jellyfish species (Aurelia aurita, Cyanea lamarckii and C. capillata within a temperate, coastal food-web in the NE Atlantic. Using information on individual size, time of year and δ13C and δ15N stable isotope values, we examined: (1 whether all jellyfish could be considered as a single functional group, or showed distinct inter-specific differences in trophic ecology; (2 Were size-based shifts in trophic position, found previously in A. aurita, a common trait across species?; (3 When considered collectively, did the trophic position of three sympatric species remain constant over time? Differences in δ15N (trophic position were evident between all three species, with size-based and temporal shifts in δ15N apparent in A. aurita and C. capillata. The isotopic niche width for all species combined increased throughout the season, reflecting temporal shifts in trophic position and seasonal succession in these gelatinous species. Taken together, these findings support previous

  8. Gelatinous macroplankton in the Black Sea in the autumn of 2010

    Science.gov (United States)

    Anninsky, B. E.; Finenko, G. A.; Datsyk, N. A.; Ignatyev, S. M.

    2013-11-01

    In October and November of 2010, the gelatinous macroplankton in the northwestern Black Sea and at the Crimean shelf was represented by the scyphozoan jellyfish Aurelia aurita, three species of ctenophores ( Beroe ovata, Mnemiopsis leidyi, and Pleurobrachia pileus), and three species of hydromedusae. A. aurita was more common at the shelf, M. leidyi and P. pileus prevailed at the deeper sea stations, and B. ovata was almost ubiquitous with a biomass from below 1 to 49 g/m2 A. aurita, which had an average biomass of 82-224 g/m2, was dominant at all the stations. M. leidyi, which had a biomass from below 1 to 115 g/m2, was recorded in October at only 14 of the 52 stations and in November at 20 of the 46 stations. The highest biomass of M. leidyi in October (105 to 116 g/m2) was recorded in the deep sea areas; in November, it was also the highest in these areas, but it reached at most 100 g/m2. The average daily ration of Aurelia ranged from 19.4 to 27.3 mg/m2 in October and from 7.0 to 17.2 mg/m2 in November; in both cases, it was insufficient to provide for the minimal physiological requirements. The average daily ration of the Mnemiopsis population (2.8-20.5 mg of zooplankton per m2) was invariably more than sufficient to provide for the minimal physiological requirements. Both predatory species together consumed less than 5% of the daily zooplankton production of the sea.

  9. Jellyfish and Ctenophores in Limfjorden (Denmark—Mini-Review, with Recent New Observations

    Directory of Open Access Journals (Sweden)

    Hans Ulrik Riisgård

    2014-10-01

    Full Text Available Limfjorden is a major Danish water system that connects the North Sea via Thyborøn Kanal in the west and to the Kattegat in the east. Limfjorden is heavily eutrophicated and certain areas suffer from oxygen depletion each summer. Bottom-dwelling fish have disappeared as the number of jellyfish and ctenophores has increased. The abundance and predation impact of jellyfish and ctenophores in Limfjorden have been described in a number of studies conducted during the last decade, and a mini-review of this literature is given here in Section 2. The common jellyfish Aurelia aurita may, in a few years, be very abundant and exert a considerable predatory impact on zooplankton and fish larvae. Abundance, species composition, and population dynamics of A. aurita may, at irregular time intervals, be strongly influenced not only by the water brought into Limfjorden from the North Sea, but also by competition with the invasive ctenophore Mnemiopsis leidyi that occurred for the first time in extremely high numbers in 2007, brought into the fjord system with North Sea water. It has been suggested that Limfjorden may function as an incubator for M. leidyi with the potential to further seed M. leidyi into the Kattegat and adjacent Danish waters, and recent observations seem to support this hypothesis. In Section 3, we report on two bloom events of ctenophores, Pleurobrachia pileus and M. leidyi, along with their predators (Beroe spp. in Limfjorden in the autumns of 2012 and 2013, when the usually dominating A. aurita was absent. However, the present observation of B. ovata being M. leidyi’s native predator and a new species in Limfjorden may in the future reduce the abundance of M. leidyi.

  10. Bioaccumulation of trace metals in shellfish and fish of Bonny River and creeks around Okrika in Rivers State, Nigeria.

    Science.gov (United States)

    Marcus, A C; Okoye, C O B; Ibeto, C N

    2013-06-01

    Lead, nickel, vanadium and cadmium were determined in fish and shellfish muscles, to assess contamination levels and identify bio-indicators. Vanadium was not detectable. Lead and cadmium were slightly above legal limits used in South East Asia, but lower than those of Australia and New Zealand. Higher contents of nickel, cadmium and lead in Pachymelania aurita and Crassostrea rhizophorae, lead in Mugil cephalus and cadmium in Periophthalmus koelreuteri, mark these species out as possible bio-indicators for the three metals in aquatic systems. Patterns of bioaccumulation seem to suggest that biophysiological and ecological characteristics influence bioaccumulation of trace metals in fish and shell fish.

  11. [Shape conservatism and shaping variability. The comparative analysis of Hydrozoa and Scyphozoa early development].

    Science.gov (United States)

    Kraus, Iu A

    2002-01-01

    The morphogenetic pathways based on the self-organization take an important part in the early development of White Sea Cnidarians--Dynamena pumila (Hydrozoa) and Aurelia aurita (Scyphozoa). Comparative analysis of their early development revealed two patterns of embryonic spatial structure reproduced in the morphogenesis of both species in spite of the differences of morphogenetic paths. These are toroidal and bilaterally symmetrical shapes. It is possible that these shapes correspond to the equilibrium states of developing system and their stable reproduction is a result of epigenetic rather than genetic program.

  12. 水族箱中的活化石观赏品种新贵——海月水母

    Institute of Scientific and Technical Information of China (English)

    李灏; 邵蓬; 丁子元

    2011-01-01

    <正>海月水母(aurelia aurita),又称水水母,在中国台湾称为幽浮水母,隶属钵水母纲旗口水母目洋须水母科海月水母属,在我国大连,烟台,青岛等沿海均有分布。源于6.5亿年前,是当今世界上历史最悠久的生物。因其婀娜多姿的泳姿和缤纷绚丽的色彩,象征着优雅的

  13. Radioactivity in three species of eastern Mediterranean jellyfish.

    Science.gov (United States)

    Mamish, S; Al-Masri, M S; Durgham, H

    2015-11-01

    Activity concentrations of (137)Cs, (40)K, (210)Po, (210)Pb, (234)U and (238)U were determined in umbrella and oral arms of three widely distributed jellyfish species; namely Rhopilema nomadica Galil, 1990, Aurelia aurita Linne, 1758 and Aequorea forskalea Péron & Lesueur, 1810 collected from February 2011 to January 2012 in four sampling locations along the Syrian coast (Eastern Mediterranean Sea). The results have shown significant variations in radionuclides activity concentrations amongst the species. The average activity concentrations of (40)K, (210)Po, (210)Pb, (234)U and (238)U in the umbrella of R. nomadica species were higher than the average activity concentrations in the umbrella of A. aurita species by about 3.2, 1.4, 1.8, 3.2 and 3.2 folds, and A. forskalea species by about 45.5, 15.4, 19, 7.4 and 7.6 folds, respectively. The average activity concentrations of (40)K, (210)Po, (210)Pb, (234)U and (238)U in oral arms of R. nomadica species were higher than the average activity concentrations in oral arms of A. aurita species by about 3.8, 1.7, 1.9, 2.8 and 2.9 folds, respectively. (137)Cs activity concentrations were below the detection limit in all measured samples. In addition, activity concentrations of (137)Cs, (40)K, (210)Po, (210)Pb, (234)U and (238)U were also determined in 44 surface seawater samples and the activity concentrations ranged between 10.6 and 11.9 Bq l(-1) for (40)K, 1.1 and 1.4 mBq l(-1) for (210)Po, 0.5 and 0.7 mBq l(-1) for (210)Pb, 40.8 and 44.5 mBq l(-1) for (234)U, and 36.9 and 38.4 mBq l(-1) for (238)U, while (137)Cs activity concentrations were below the detection limit in all measured samples. Moreover, the umbrella and oral arms readily accumulated (40)K, (210)Po, (210)Pb, (234)U and (238)U above ambient seawater levels in the sequence of (210)Po > (210)Pb > (4) K > (234)U and (238)U. Concentration ratio (CR) values were relatively high for (210)Po and (210)Pb and reached 10(3) and 10(2), respectively for the jellyfish R

  14. Kinematics and Fluid Dynamics of Jellyfish Maneuvering

    Science.gov (United States)

    Miller, Laura; Hoover, Alex

    2014-11-01

    Jellyfish propel themselves through the water through periodic contractions of their elastic bells. Some jellyfish, such as the moon jellyfish Aurelia aurita and the upside down jellyfish Cassiopea xamachana, can perform turns via asymmetric contractions of the bell. The fluid dynamics of jellyfish forward propulsion and turning is explored here by analyzing the contraction kinematics of several species and using flow visualization to quantify the resulting flow fields. The asymmetric contraction and structure of the jellyfish generates asymmetries in the starting and stopping vortices. This creates a diagonal jet and a net torque acting on the jellyfish. Results are compared to immersed boundary simulations

  15. Levels of selected metals in canned fish consumed in Kingdom of Saudi Arabia.

    Science.gov (United States)

    Ashraf, Waqar; Seddigi, Zaki; Abulkibash, Abdallah; Khalid, Mazen

    2006-06-01

    In the present paper, seven heavy metals (Pb, Cd, Ni, Cu, Zn, Cr and Fe) in canned salmon, sardine and tuna fish were determined by using atomic absorption spectroscopy. Cadmium and lead levels were determined by graphite tube AAS whereas Ni, Cu, Cr and Fe were determined by flame AAS. Analytical results were validated by spiking the samples with various concentrations of these metals for recovery. The metal contents, expressed in microg/g, wet weight, varied depending upon the specie studied. The levels of Pb ranged from 0.03-1.20 microg-g(-1) with an average of 0.313 microg-g(-1) for salmon; 0.03-0.51 microg-g(-1) with an average of 0.233 microg-g(-1) for tuna and 0.13-1.97 microg-g(-1) with an average of 0.835 microg-g(-1) for sardines. The levels of Cd ranged from 0.02-0.38 microg-g(-1) with an average of 0.161 microg-g(-1) for salmon; 0.07-0.64 microg-g(-1) with an average of 0.227 microg-g(-1) for tuna and 0.010-0.690 microg-g(-1) with an average of 0.183 microg-g(-1) for sardines. Comparative evaluation of these metals in three varieties of fish showed that average concentration of lead in sardines is about 4 times and Ni about 3 times higher as compared to tuna. Generally, the levels of these metals follow the order sardine > salmon > tuna. The data generated in the present study compared well with the similar studies carried out in different parts of the world. The results indicate that canned fish, in general and tuna in particular, have concentrations within permissible limits of WHO/FAO levels for these heavy metals. Therefore, their contribution to the total body burden of these metals can be considered as negligibly small.

  16. Elucidating dynamic responses of North Pacific fish populations to climatic forcing: Influence of life-history strategy

    Science.gov (United States)

    Yatsu, A.; Aydin, K. Y.; King, J. R.; McFarlane, G. A.; Chiba, S.; Tadokoro, K.; Kaeriyama, M.; Watanabe, Y.

    2008-05-01

    In order to explore mechanistic linkages between low-frequency ocean/climate variability, and fish population responses, we undertook comparative studies of time-series of recruitment-related productivity and the biomass levels of fish stocks representing five life-history strategies in the northern North Pacific between the 1950s and the present. We selected seven species: Japanese sardine ( Sardinopus melanostictus) and California sardine ( Sardinopus sagax) (opportunistic strategists), walleye pollock ( Theragra chalcogramma, intermediate strategist), pink salmon ( Oncorhynchus gorbuscha, salmonic strategist), sablefish ( Anoplopoma fimbria) and Pacific halibut ( Hippoglossus stenolepis) (periodic strategists) and spiny dogfish ( Squalus acanthias, equilibrium strategist). The responses in terms of productivity of sardine, pink salmon, sablefish and halibut to climatic regime shifts were generally immediate, delayed, or no substantial responses depending on the particular regime shift year and fish stock (population). In walleye pollock, there were some periods of high productivity and low productivity, but not coincidental to climatic regime shifts, likely due to indirect climate forcing impacts on both bottom-up and top-down processes. Biomass of zooplankton and all fish stocks examined, except for spiny dogfish whose data were limited, indicated a decadal pattern with the most gradual changes in periodic strategists and most intensive and rapid changes in opportunistic strategists. Responses of sardine productivity to regime shifts were the most intense, probably due to the absence of density-dependent effects and the availability of refuges from predators when sardine biomass was extremely low. Spiny dogfish were least affected by environmental variability. Conversely, spiny dogfish are likely to withstand only modest harvest rates due to their very low intrinsic rate of increase. Thus, each life-history strategy type had a unique response to climatic

  17. Estructura territorial de la actividad pesquera en Guaymas, Sonora

    Directory of Open Access Journals (Sweden)

    Gonzalo Yurkievich

    2016-11-01

    declined in subsequent years, mostly due to the overexploitation of shrimp banks and the incorporation of private capital investors to the local shrimp economy; the downward trend of this fishery in Guaymas is still evident. For its part, sardine, particularly Monterey sardine, is the other important species in the economy in this sea port. It is captured to meet the demand for oil and fish meal. Since the 1960s, Guaymas has become Mexico’s sardine port, as this species is at the core of the territorial structure of fisheries in this city. A significant volume of sardine biomass has been captured in the last 50 years in Guaymas, resulting in the overexploitation of the resource which, along with water pollution, has led to the collapse of sardine populations. Nonetheless, stakeholders and authorities involved this fishery have obtained the international certification of local sardine catches. There are several enterprises located in Guaymas dedicated to the industrial processing of sardine. These are vertically integrated, so they can easily buy catches, process them and sell the manufactured products both in Mexico and abroad. Markets for these and other fishing products are located at a considerable distance from Guaymas, including Mexico City, Guadalajara, Mexicali and Chihuahua City, as well and several places in California, United States. The territorial structure of the fishing economy in this part of Mexico is arranged on a spatial platform constituted by high-volume and diverse annual catches, an integrated sea port that not only addresses the needs of this economy but also serves the robust industrial and agricultural sectors of Sonora. Shipyards, large enterprises (national and foreign involved in the fishing industry, banks, academic research centers and government offices can be added to the local fishing infrastructure. The geographical reach of the commercialization of fishing products (processed or otherwise, including several markets located far from this

  18. The Argentinean dung beetle genus Anomiopsoides (Scarabaeidae: Scarabaeinae: Eucraniini: description of a new species, and new synonymies for A. heteroclyta El género argentino de escarabajos estercoleros Anomiopsoides (Scarabaeidae: Scarabaeinae: Eucraniini: descripcción de una especie nueva y nuevas sinonimias para A. heteroclyta

    Directory of Open Access Journals (Sweden)

    Federico C. Ocampo

    2007-12-01

    Full Text Available The taxonomy of the genus Anomiopsoides Blackwelder is revised. The species A. catamarcae Martínez and A. aurita (Burmeister are placed in synonymy with A. heteroclyta (Blanchard. Anomiopsoides fedemariai sp. nov. is described from Argentina. The genus Anomiopsoides now consists of four species: A. biloba (Burmeister, A. cavifrons (Burmeister, A. fedemariai sp. nov. and A. heteroclyta (Blanchard. A key is presented for the identification of the species of Anomiopsoides.Se hace una revisión de la taxonomía del género Anomiopsoides Blackwelder. Las especies A. catamarcae Martínez y A. aurita (Burmeister son consideradas como sinónimos de A. heteroclyta (Blanchard. Se describe una especie nueva, A. fedemariai sp. nov., especie nueva de Argentina. El género Anomioposoides consiste ahora en cuatro especies, A. biloba (Burmeister, A. cavifrons (Burmeister, A. fedemariai sp. nov. y A. heteroclyta (Blanchard. Se presenta una clave para la identificación de las especies de Anomiopsoides.

  19. Kuzey Ege ve Marmara Denizi Kıyısal Yüzey Sularında Scyphomedusae ve Ctenophora Üzerine Yerinde Gözlemler.

    Directory of Open Access Journals (Sweden)

    Süleyman Mavili

    2015-12-01

    Full Text Available Skifomedüz ve ktenoforların dağılımı ve bolluğu, 1998 ve 2005 arasında yaz ve sonbahar dönemlerinde yerinde gözlemlerle incelenmiştir. Skifomedüzler (A. aurita, R. pulmo Kuzey Ege ve Marmara Denizi’nde geniş bir dağılım göstermiştir. Yazın baskın olan A. aurita sonbaharda azalmış, oysa R. pulmo bu dönemde belirgin olarak artmıştır. Ktenoforlar (M. leidyi, B. ovata Kuzey Ege’de bulunmamıştır. M. leidyi Marmara Denizi’nde 1999’da bol olarak bulunmuş, B. ovata’nın ortaya çıktığı 2000’de belirgin bir azalma göstermiştir. Her iki ktenofor da 2004 ve 2005’de çalışma bölgesinde bulunmamıştır. Bu da pek muhtemelen M. leidyi yoğunluğunda büyük bir azalmaya işaret etmektedir

  20. Scyphozoan jellyfish venom metalloproteinases and their role in the cytotoxicity.

    Science.gov (United States)

    Lee, Hyunkyoung; Jung, Eun-sun; Kang, Changkeun; Yoon, Won Duk; Kim, Jong-Shu; Kim, Euikyung

    2011-09-01

    The present study, for the first time, comparatively investigated the enzymatic activities (proteases and hyaluronidases) in the venoms of four Scyphozoan jellyfish species, including Nemopilema nomurai, Rhopilema esculenta, Cyanea nozakii, and Aurelia aurita. For this, various zymographic analyses were performed using assay specific substrates. Interestingly, all the four jellyfish venoms showed gelatinolytic, caseinolytic, and fibrinolytic activities, each of which contains a multitude of enzyme components with molecular weights between 17 and 130 kDa. These four jellyfish venoms demonstrated a huge variation in their proteolytic activities in quantitative and qualitative manner depending on the species. Most of these enzymatic activities were disappeared by the treatment of 1,10-phenanthroline, suggesting they might be belonged to metalloproteinases. Toxicological significance of these venom proteases was examined by comparing their proteolytic activity and the cytotoxicity in NIH 3T3 cells. The relative cytotoxic potency was C. nozakii > N. nomurai > A. aurita > R. esculenta. The cytotoxicity of jellyfish venom shows a positive correlation with its overall proteolytic activity. The metalloproteinases appear to play an important role in the induction of jellyfish venom toxicities. In conclusion, the present report proposes a novel finding of Scyphozoan jellyfish venom metalloproteinases and their potential role in the cytotoxicity.

  1. Jellyfish mucin may have potential disease-modifying effects on osteoarthritis

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    Urai Makoto

    2009-12-01

    Full Text Available Abstract Background We aimed to study the effects of intra-articular injection of jellyfish mucin (qniumucin on articular cartilage degeneration in a model of osteoarthritis (OA created in rabbit knees by resection of the anterior cruciate ligament. Qniumucin was extracted from Aurelia aurita (moon jellyfish and Stomolophus nomurai (Nomura's jellyfish and purified by ion exchange chromatography. The OA model used 36 knees in 18 Japanese white rabbits. Purified qniumucin extracts from S. nomurai or A. aurita were used at 1 mg/ml. Rabbits were divided into four groups: a control (C group injected with saline; a hyaluronic acid (HA-only group (H group; two qniumucin-only groups (M groups; and two qniumucin + HA groups (MH groups. One milligram of each solution was injected intra-articularly once a week for 5 consecutive weeks, starting from 4 weeks after surgery. Ten weeks after surgery, the articular cartilage was evaluated macroscopically and histologically. Results In the C and M groups, macroscopic cartilage defects extended to the subchondral bone medially and laterally. When the H and both MH groups were compared, only minor cartilage degeneration was observed in groups treated with qniumucin in contrast to the group without qniumucin. Histologically, densely safranin-O-stained cartilage layers were observed in the H and two MH groups, but cartilage was strongly maintained in both MH groups. Conclusion At the concentrations of qniumucin used in this study, injection together with HA inhibited articular cartilage degeneration in this model of OA.

  2. Seasonal Investigations into the Level of Toxic Elements in Marine Organisms (Fish and Mollusk along the Coast of Ghana Using Neutron Activation Analysis

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    L.A. Sarsah

    2011-04-01

    Full Text Available Seasonal investigation was conducted into the occurrence and extent of potentially toxic heavy metals along the coast of Ghana using marine organisms as bioindicators of pollution. The marine organisms sampled were analysed using Instrumental Neutron Activation Analysis coupled with conventional counting system. All the four samples (Dentex macrophthalmus, Sardinella maderensis, Engraulis encrasicolus and Cymbium cymbium recorded detectable levels of potentially toxic elements which fluctuates between <0.07mg/kg Cd and 699 mg/kg Al. Cymbium cymbium recorded the highest level of (As, Co, Cu, Zn whiles Engraulis encrasicolus accumulated appreciable amount of V. Al was the most abundant of all the elements. The coast of Ghana was found to be mildly polluted with respect to the elements Al, As, Cd, Co, Cu, Cr, Ni, Hg, V and Zn.

  3. The utilization of crude fish oil (CFO) to increase mudcrab (Scylla serrata) feed quality

    Science.gov (United States)

    Lamid, Mirni; Agustono

    2017-02-01

    Crude fish oil is one of essential fatty acid sources, which is found in Sardinella lemuru. This research aims to study the quality improvement of mudcrab(Scylla serrata) feed. Four feed formulations were designed by using completely randomized design, including P0 = trash fish + 1% tapioca starch; P1=trash fish + 2.0% crude fish oil + 1% tapioca starch;, P2= trash fish +4.0% crude fish oil + 1% tapioca starch; P3=trash fish + 6.0% crude fish oil + 1% tapioca starch; P4=trash fish +8.0% crude fish oil + 1% tapioca starch, respectively, which were carried out in quadruplicate. This study showed that feed formulation significantly affected crude protein, crude fiber, crude lipid, ash, organic matter and nitrogen free extract and energy of mudcrab. The P2 feed was the best formulation but had a slight different from P3 formulation.

  4. Disponibilité en poissons et pouvoir d'achat de la population dans la région du moyen Comoé (Côte-d'lvoire

    Directory of Open Access Journals (Sweden)

    Vanga, AF.

    2000-01-01

    Full Text Available Fish Availability and Buying-power of the Local Population in the Central Comoe Region (Ivory Coast. The origin of commercial fish found on local markets in the "Moyen Comoe" area is diverse : imported frozen fish, fish coming from Abidjan and smoked in Akoupe (South Ivory Coast, locally caught fish and fish caught in neighbouring countries (Ghana and Mali. The atlantic horse mackerel and the round sardinella (frozen fish are the most important commercial fish species found on the markets. These fish, sold at 350 francs CFA, can be afforded by the majority of the households, 82 % of which spend less than 1000 francs CFA for buying fish and/or meat (beaf.

  5. Overfishing of small pelagic fishes increases trophic overlap between immature and mature striped dolphins in the Mediterranean Sea.

    Science.gov (United States)

    Gómez-Campos, Encarna; Borrell, Assumpció; Cardona, Luis; Forcada, Jaume; Aguilar, Alex

    2011-01-01

    The interactions among diet, ecology, physiology, and biochemistry affect N and C stable isotope signatures in animal tissues. Here, we examined if ecological segregation among animals in relation to sex and age existed by analyzing the signatures of δ(15)N and δ(13)C in the muscle of Western Mediterranean striped dolphins. Moreover, we used a Bayesian mixing model to study diet composition and investigated potential dietary changes over the last two decades in this population. For this, we compared isotope signatures in samples of stranded dolphins obtained during two epizootic events occurring in 1990 and 2007-2008. Mean δ(13)C values for females and males were not significantly different, but age-related variation indicated δ(13)C enrichment in both sexes, suggesting that females and males most likely fed in the same general areas, increasing their consumption of benthic prey with age. Enrichment of δ(15)N was only observed in females, suggesting a preference for larger or higher trophic level prey than males, which could reflect different nutritional requirements. δ(13)C values showed no temporal variation, although the mean δ(15)N signature decreased from 1990 to 2007-2008, which could indicate a dietary shift in the striped dolphin over the last two decades. The results of SIAR indicated that in 1990, hake and sardine together contributed to 60% on the diet of immature striped dolphins, and close to 90% for mature striped dolphins. Conversely, the diet of both groups in 2007-2008 was more diverse, as hake and sardine contributed to less than 40% of the entire diet. These results suggest a dietary change that was possibly related to changes in food availability, which is consistent with the depletion of sardine stocks by fishing.

  6. Dynamic height: A key variable for identifying the spawning habitat of small pelagic fishes

    Science.gov (United States)

    Asch, Rebecca G.; Checkley, David M., Jr.

    2013-01-01

    Small pelagic fishes off southern California exhibit interannual variations in the regions they occupy. An enhanced understanding of these fluctuations could improve fisheries management and predictions of fish's responses to climate change. We investigated dynamic height as a variable for identifying the spawning habitat of northern anchovy (Engraulis mordax), Pacific sardine (Sardinops sagax), and jack mackerel (Trachurus symmetricus). During cruises between 1998 and 2004, dynamic height was calculated from temperature and salinity profiles, while fish egg concentration was measured with obliquely towed bongo nets and the Continuous, Underway Fish Egg Sampler. Dynamic height ranged between 68 and 108 cm, with values increasing offshore. The greatest probability of encountering anchovy, sardine, and jack mackerel eggs occurred at dynamic heights of 79-83 cm, 84-89 cm, and 89-99 cm, respectively. Four mechanisms were proposed to explain how dynamic height affects egg distribution: (1) dynamic height is a proxy for upper water column temperature and salinity, which are known to influence spawning habitat. (2) Low dynamic heights are indicative of coastal upwelling, which increases primary and secondary productivity. (3) Egg concentration is greater at dynamic heights coincident with geostrophic currents that transport larvae to favorable habitats. (4) Eddies delineated by dynamic height contours retain eggs in productive habitats. To evaluate these mechanisms, a generalized linear model was constructed using dynamic height, temperature, salinity, chlorophyll, zooplankton volume, geostrophic currents, and eddies as independent variables. Dynamic height explained more variance than any other variable in models of sardine and anchovy spawning habitat. Together temperature, salinity, and chlorophyll accounted for 80-95% of the dynamic height effect, emphasizing the importance of the first two mechanisms. However, dynamic height remained statistically significant in the

  7. Spatio-temporal patterns and environmental controls of small pelagic fish body condition from contrasted Mediterranean areas

    Science.gov (United States)

    Brosset, Pablo; Fromentin, Jean-Marc; Van Beveren, Elisabeth; Lloret, Josep; Marques, Virginie; Basilone, Gualtiero; Bonanno, Angelo; Carpi, Piera; Donato, Fortunata; Čikeš Keč, Vanja; De Felice, Andrea; Ferreri, Rosalia; Gašparević, Denis; Giráldez, Ana; Gücü, Ali; Iglesias, Magdalena; Leonori, Iole; Palomera, Isabel; Somarakis, Stylianos; Tičina, Vjekoslav; Torres, Pedro; Ventero, Ana; Zorica, Barbara; Ménard, Frédéric; Saraux, Claire

    2017-02-01

    Small pelagic fish are among the most ecologically and economically important marine fish species and are characterized by large fluctuations all over the world. In the Mediterranean Sea, low catches and biomass of anchovies and sardines have been described in some areas during the last decade, resulting in important fisheries crises. Therefore, we studied anchovy and sardine body condition variability, a key index of population health and its response to environmental and anthropogenic changes. Wide temporal and spatial patterns were investigated by analyzing separately data from scientific surveys and fisheries in eight Mediterranean areas between 1975 and 2015. Results showed that anchovy and sardine body condition as well as maximum size in some areas sharply decreased in most Mediterranean areas along years (except in the Northern Alboran Sea). Despite this general pattern, well-marked environmental differences between sub-regions were highlighted by several analyses and variations in body condition were not found to be homogeneous over all the Mediterranean Sea. Further, other analyses revealed that except for the Adriatic where major changes towards a lower body condition were concomitant with a decrease in river runoffs and chl-a concentration, no concomitant environmental regime shift was detected in other areas. Together, these analyses highlighted the current poor body condition of almost all small pelagic fish populations in the Mediterranean. Yet, global environmental indices could not explain the observed changes and the general decrease in condition might more likely come from regional environmental and/or anthropogenic (fishing) effects. A prolonged state of poor fish body condition, together with an observed reduced size and early age-at-maturity may have strong ecological, economic and social consequences all around the Mediterranean Sea.

  8. Body reserves mediate trade-offs between life-history traits: new insights from small pelagic fish reproduction.

    Science.gov (United States)

    Brosset, Pablo; Lloret, Josep; Muñoz, Marta; Fauvel, Christian; Van Beveren, Elisabeth; Marques, Virginie; Fromentin, Jean-Marc; Ménard, Frédéric; Saraux, Claire

    2016-10-01

    Limited resources in the environment prevent individuals from simultaneously maximizing all life-history traits, resulting in trade-offs. In particular, the cost of reproduction is well known to negatively affect energy investment in growth and maintenance. Here, we investigated these trade-offs during contrasting periods of high versus low fish size and body condition (before/after 2008) in the Gulf of Lions. Female reproductive allocation and performance in anchovy (Engraulis encrasicolus) and sardine (Sardina pilchardus) were examined based on morphometric historical data from the 1970s and from 2003 to 2015. Additionally, potential maternal effects on egg quantity and quality were examined in 2014/2015. After 2008, the gonadosomatic index increased for sardine and remained steady for anchovy, while a strong decline in mean length at first maturity indicated earlier maturation for both species. Regarding maternal effects, for both species egg quantity was positively linked to fish size but not to fish lipid reserves, while the egg quality was positively related to lipid reserves. Atresia prevalence and intensity were rather low regardless of fish condition and size. Finally, estimations of total annual numbers of eggs spawned indicated a sharp decrease for sardine since 2008 but a slight increase for anchovy during the last 5 years. This study revealed a biased allocation towards reproduction in small pelagic fish when confronted with a really low body condition. This highlights that fish can maintain high reproductive investment potentially at the cost of other traits which might explain the present disappearance of old and large individuals in the Gulf of Lions.

  9. Overfishing of small pelagic fishes increases trophic overlap between immature and mature striped dolphins in the Mediterranean Sea.

    Directory of Open Access Journals (Sweden)

    Encarna Gómez-Campos

    Full Text Available The interactions among diet, ecology, physiology, and biochemistry affect N and C stable isotope signatures in animal tissues. Here, we examined if ecological segregation among animals in relation to sex and age existed by analyzing the signatures of δ(15N and δ(13C in the muscle of Western Mediterranean striped dolphins. Moreover, we used a Bayesian mixing model to study diet composition and investigated potential dietary changes over the last two decades in this population. For this, we compared isotope signatures in samples of stranded dolphins obtained during two epizootic events occurring in 1990 and 2007-2008. Mean δ(13C values for females and males were not significantly different, but age-related variation indicated δ(13C enrichment in both sexes, suggesting that females and males most likely fed in the same general areas, increasing their consumption of benthic prey with age. Enrichment of δ(15N was only observed in females, suggesting a preference for larger or higher trophic level prey than males, which could reflect different nutritional requirements. δ(13C values showed no temporal variation, although the mean δ(15N signature decreased from 1990 to 2007-2008, which could indicate a dietary shift in the striped dolphin over the last two decades. The results of SIAR indicated that in 1990, hake and sardine together contributed to 60% on the diet of immature striped dolphins, and close to 90% for mature striped dolphins. Conversely, the diet of both groups in 2007-2008 was more diverse, as hake and sardine contributed to less than 40% of the entire diet. These results suggest a dietary change that was possibly related to changes in food availability, which is consistent with the depletion of sardine stocks by fishing.

  10. Navodon septentrionalis Predation of Four Species of Giant Jellyfish%绿鳍马面鲀(Navodon septentrionalis)对4种大型水母的捕食行为

    Institute of Scientific and Technical Information of China (English)

    刘春胜; 庄志猛; 陈四清; 刘长琳; 赵鹏; 陈昭廷

    2014-01-01

    以绿鳍马面鲀为研究对象,通过室内受控实验,比较研究了其对我国沿海4种常见大型水母(海蜇、沙海蜇、海月水母和白色霞水母)的捕食差异。结果显示,体重为(215±20) g的绿鳍马面鲀对海月水母的捕食能力最强,日均最大摄食量为(150.7±18.6) g/fish,其次是海蜇和白色霞水母,日均摄食量分别为(129.7±11.6)和(120.0±19.3) g/fish,对沙海蜇的摄食量最少,为(92.5±11.3) g/fish;绿鳍马面鲀对海月水母与海蜇摄食量主要受投喂量影响,与规格无关,当投喂量小于其最大捕食量时,绿鳍马面鲀可捕食其周围所有水母,当投喂量超过其最大摄食量并继续增加时,绿鳍马面鲀摄食量保持不变,但残余水母的触手和伞部边缘均被啃食,继而导致水母摄食能力丧失,难以继续生存;在适口饵料冰鲜玉筋鱼充足的情况下,绿鳍马面鲀对水母具有明显的摄食偏向性,与仅投喂水母实验组相比,其对海月水母和海蜇的日均摄食量仅降低了20.2%和16.9%。研究结果表明,绿鳍马面鲀对上述4种水母皆能捕食。%The predatory behavior of filefish Navodon septentrionalis to Aurelia aurita was observed before. Here we hypothesized that there were also predator-prey relationships between filefish and other jellyfish species. We explored the predatory behaviors of filefish to four species of giant jellyfish, including Rhopilema esculentum, Nemopilema nomurai, A. aurita and Cyanea nozakii, under laboratory conditions. We also studied the prey selection of filefish among jellyfish species of R. esculentum and A. aurita, and sand lance. Filefish weighted (215±20) g actively preyed on all four jellyfish species, and its daily medusae consumption of A. aurita, R. esculentum, C. nozakii, and N. nomurai was 150.7±18.6, 129.7±11.6, 120.0±19.3, and 92.5±11.3 g/fish respectively. It was the medusae biomass, not the bell diameter that

  11. [Occurrence of residues of organochlorine pesticides, nitromusk compounds and polychlorobiphenyls in Turkish canned fish products].

    Science.gov (United States)

    Ozden, O; Kruse, R; Erkan, N

    2001-04-01

    In our study we elaborated an overview on the contamination grade of fish from Turkish waters by selected organochlorine pesticides, nitromusks and chlorobiphenyl congenres. We could demonstrate, that the tested fish species, being prior processed to canned products and brought to the market, contained particularly unmistakable amounts of the above mentioned analytes. Sardines, sardelles and trout gave results generally far below the German regulatory limits. However total DDT in pelamides reached an order of magnitude near the German limit of 0.5 mg/kg (based on wet weight). The necessity of enhanced systematic measurements for monitoring pollutants in fish from Turkish waters thus becomes evident.

  12. Gioco e Avanguardia: non siamo tutti un po’ Dada?

    DEFF Research Database (Denmark)

    Prager, Phillip

    2015-01-01

    Il Dadaismo, movimento artistico che si affermò alla fine del 1910 e nei primi anni Venti, sfidò le tradizionali nozioni di arte ed estetica. I dadaisti, per esempio, gettavano ritagli di carta colorata in aria per comporre ipotetici collage, eseguivano poesie prive di valore semantico e creavano...... cappelli formati da scatole di sardine. Per la maggior parte degli storici dell‟arte, il Dadaismo rimane culturalmente una espressione legata in maniera contingente alla Prima guerra mondiale, al trauma, al nichilismo, alla disillusione politica e ad un attacco aggressivo alla bancarotta morale della...

  13. Lipid metabolism in experimental animals

    Directory of Open Access Journals (Sweden)

    Sánchez-Muñiz, Francisco J.

    1998-08-01

    Full Text Available Publications are scarce in the way in chich metabolic processes are affected by the ingestion of heated fats used to prepare food. Similarly studies measuring metabolic effects of the consumption on fried food are poorly known. The purpose of this presentation is to summarize information on frying fats and frying foods upon lipid metabolism in experimental animals. Food consumption is equivalent or even higher when oils or the fat content of frying foods are poorly alterated decreasing their acceptability when their alteration degree increase. After 4hr. experiment the digestibility and absorption coefficients of a single dosis of thermooxidized oils were significantly decreased in rats, however the digestive utilization of frying thermooxidized oils included in diets showed very little change in comparison with unused oils by feeding trials on rats. Feeding rats different frying fats induced a slight hypercholesterolemic effect being the magnitude of this effect related to the linoleic decrease in diet produced by frying. However HDL, the main rat-cholesterol carrier, also increased, thus the serum cholesterol/HDL-cholesterol ratio did not change. Results suggest that rats fed frying fats adapt their lipoprotein metabolism increasing the number of HDL particles. Deep fat frying deeply changed the fatty acid composition of foods, being possible to increase their n-9 or n-6 fatty acid and to decrease the saturated fatty acid contents by frying. When olive oil-and sunflower oil-fried sardines were used as the only protein and fat sources of rats-diets in order to prevent the dietary hypercholesterolemia it was provided that both fried-sardine diets showed a powerful check effect on the cholesterol raising effect induced by dietary cholesterol. The negative effect of feeding rats cholesterol plus bovine bile to induce hypercholesterolemia on some cell-damage markers such as lactate dehydrogenase, transaminases, alkaline phosphatase, was

  14. Fishing activity of small pelagic in Albania: 1948-1990

    Directory of Open Access Journals (Sweden)

    ROLAND KRISTO

    2014-03-01

    Full Text Available The small pelagic species are important commercial fishes of the Adriatic Sea. In Albania, the fishing activity of Sardines had a key importance still in 1990, as it represented about 70% of total catch. The main fishing method used was purse seine using light attraction that represented about 80% of total catches of sardines and the rest was mid-water pelagic trawl nets towed by two vessels. Albanian catches of sardines reached a maximum value, in 1982-1985 with value from 5,000 to 6,500 tons and maximum at 1984 with 6,596 tons. In 1990, the Sardine fleet consisted of 60 fishing vessels, from which 28 in Vlora, 12 in Durres, 12 in Shengjin and 8 in Saranda. The number of fishing vessels for bottom trawl fishing was as average 21 and 23 for 1985 and 1989 respectively, with a maximum of 28 fishing vessels. The average of fishing days was about 142 days/year. The production for 1985 was 1899.6 tons and for 1989 was 2324.2 tons. The number of fishing vessels for mid water pelagic pair trawls was as average 17 and 8 for 1985 and 1989 respectively, with a maximum of 27 and 16 fishing vessels. The production for 1985 was 2230.2 tons and for 1989 was 744.6 tons. The number of fishing vessels with purse seines with light attraction was as average 34 and 37 for 1985 and 1989 respectively, with a maximum of 38 and 45 fishing vessels. The average of fishing days was about 137 days/year. The production for 1985 and 1989 was 3319.2 and 2919.8 tons respectively. The number of coastal fishing vessels was as average 13 and 15 for 1985 and 1989 respectively, with a maximum of 14 and 19 fishing vessels. The average of fishing days was about 109 days/year. The production for 1985 and 1989 was 166.7 and 188.9 tons respectively

  15. Desenvolvimento Pós-embrionário de Ophyra aenescens (Wiedemann, 1830 (Diptera: Muscidae em Diferentes Dietas, sob Condições de Laboratório

    Directory of Open Access Journals (Sweden)

    José Mario d'Almeida

    1999-01-01

    Full Text Available Post-embryonic Development of Ophyra aenescens (Wiedemann, 1830 (Diptera: Muscidae, in Different Diets, under Laboratory Conditions - The performance of various diets (bovine meat, fish- sardine, shrimp, dog faeces, and banana in Ophyra aenescens development was evaluated. The biology was studied in an incubator (BOD at 27±1oC and 80±10% of RH. The developmental time from larvae to adult, the developmental time and viability of larvae and pupae, the weight of pupae as well as the sex ratio of the emerging adults were also determined. Beef and shrimp were the more efficient diets for rearing O. aenescens.

  16. Play and the Avant-Garde: Aren't We All a Little Dada?

    DEFF Research Database (Denmark)

    Prager, Phillip

    2013-01-01

    , and modeled a headpiece fashioned of sardine cans. To most art historians, Dada remains a culturally contingent expression of World War I trauma, nihilism, political disillusionment, and an aggressive attack on the moral bankruptcy of Western culture. The author suggests that this negative interpretation...... originates from art history’s methodological blindness to the importance of play, not only to creative and artistic endeavors, but to human identity itself. Dada is characterized by an effervescent love of improvisation, curiosity, novelty and an unselfconscious exploration of the phenomenal world...

  17. Synthesis of structured triacylglycerols enriched in n-3 fatty acids by immobilized microbial lipase

    OpenAIRE

    Maria Elisa Melo Branco de Araújo; Paula Renata Bueno Campos; Thiago Grando Alberto; Fabiano Jares Contesini; Patrícia de Oliveira Carvalho

    2016-01-01

    Abstract The search for new biocatalysts has aroused great interest due to the variety of micro-organisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sardine oil in solvent-free media. For the immobilization process, the best lipase/support ratios were 1:3 (w/w) for Aspergillus niger lipa...

  18. PATHOGENIC MICROORGANISMS ISOLATED FROM PERIWINKLES IN CREEKS SOUTH-SOUTH OF NIGERIA

    Directory of Open Access Journals (Sweden)

    P. NWIYI

    2013-07-01

    Full Text Available One hundred and twenty pieces of periwinkle were obtain each from Yenogoa and Oron Creek. The periwinkle were of two genera namely: Pachymelania aurita obtained from Oronk Creek located in Akwa-Ibom State, while the Tympanotonus fuscatus notably a brackish water habitat was obtained from Yenogoa in Bayelsa state both in south-south Nigeria. Evaluation of possible microbiological isolate was carried out according to Cowan and Steel’s Manuel for medical Bacterial identification. The Creek in Yenogoa presented high level of Coliform count 2.6×105cfug-1 while the Oron Creek had an unacceptable load of Salmonella count 6×106cfug-1. The total bacterial count was highest in Oron Creek 1.46×108cfug-1 from Tympanotonus fuscatus. The microorganisms isolated from both Creeks were Esherichia coli, proteus sp, salmonella sp, pseudomonas sp and Enterobacter sp. Proteus sp was the least isolated while Salmonella sp was the highest.

  19. Toxoplasma gondii antibodies in wild rodents and marsupials from the Atlantic Forest, state of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Solange Maria Gennari

    Full Text Available Toxoplasma gondii is a protozoan parasite that infects a large spectrum of warm-blooded animals, including humans. Small rodents and marsupials play an important role in the epidemiology of T. gondii because they are sources of infection for domestic and feral cats. Serum samples from 151 rodents and 48 marsupials, captured in the Atlantic Forest, São Paulo State, southeastern Brazil, were analyzed for the presence of T. gondii antibodies. Antibodies detected by the modified agglutination test (MAT ≥ 25 were found in 8.6% (13/151 of the rodents and 10.4% (5/48 of the marsupials, with titers ranging from 25 to 6400 and from 25 to 3200, respectively for the rodents and marsupials. Three of the eight species of rodents (Akodon spp., Oligoryzomys nigripesand Rattus norvegicus, and one from the four marsupial species (Didelphis aurita presented positive animals. T. gondii was described for the first time in the rodent Oligoryzomys nigripes.

  20. Gramíneas (Poaceae da Área de Relevante Interesse Ecológico (ARIE "Santuário de Vida Silvestre do Riacho Fundo", Distrito Federal, Brasil

    Directory of Open Access Journals (Sweden)

    Rodrigues-da-Silva Robson

    2003-01-01

    Full Text Available Neste trabalho relata-se o levantamento florístico das espécies de Poaceae da Área de Relevante Interesse Ecológico (ARIE "Santuário de Vida Silvestre do Riacho Fundo", Distrito Federal, Brasil. Foram encontradas 107 espécies, distribuídas em 41 gêneros, sendo 82 nativas e 25 exóticas. São apresentadas chaves analíticas para gêneros e espécies, além de ilustrações para os táxons identificados. Coelorachis aurita (Steud. A. Camus é citada pela primeira vez para a flora do Distrito Federal.

  1. Description of Leishmania (Leishmania forattinii sp. n., a new parasite infecting opossums and rodents in Brazil

    Directory of Open Access Journals (Sweden)

    Elizaide L. A. Yoshida

    1993-09-01

    Full Text Available A new parasite species of Leishmania is described, L. (Leishmania forattinii sp. n., which was isolated from a pooled triturate of liver and spleen of a opossum (Didelphis marsupialis aurita and from skin samples from a rodent (Proechmys iheringi denigratus, captured in primary forest on the Atlantic Cost of Brazil. Our results on the basis of biological and molecular criteria indicate that this taxonomically distinct parasite ias a new species of the L. mexicana complex, but closely related to L. (L. aristidesi Laison & shaw, 1979, as revelated by phenetic and phylogenetic numerical analyses of the enzyme data. L. forattinii was clearly distinguishable from other Leishmania species of the genus usisng enzyme electrophoresis, monoclonal antibodies, molecular karyotypes, analysis of restriction enzyme digestion patterns of kinetoplast DNA (kDNA, as well as the use of kDNA hybridization procedures.

  2. On the dynamics of jellyfish locomotion via 3D particle tracking velocimetry

    Science.gov (United States)

    Piper, Matthew; Kim, Jin-Tae; Chamorro, Leonardo P.

    2016-11-01

    The dynamics of jellyfish (Aurelia aurita) locomotion is experimentally studied via 3D particle tracking velocimetry. 3D locations of the bell tip are tracked over 1.5 cycles to describe the jellyfish path. Multiple positions of the jellyfish bell margin are initially tracked in 2D from four independent planes and individually projected in 3D based on the jellyfish path and geometrical properties of the setup. A cubic spline interpolation and the exponentially weighted moving average are used to estimate derived quantities, including velocity and acceleration of the jellyfish locomotion. We will discuss distinctive features of the jellyfish 3D motion at various swimming phases, and will provide insight on the 3D contraction and relaxation in terms of the locomotion, the steadiness of the bell margin eccentricity, and local Reynolds number based on the instantaneous mean diameter of the bell.

  3. Notas sôbre a evolução do virus vacínico em animais silvestres da fauna brasileira

    Directory of Open Access Journals (Sweden)

    Cicero Alves Moreira

    1956-06-01

    Full Text Available A note on the evolution of cow-pox virus in wild animals of Brazilian fauna. We have tried the sensibility of wild animals of Brazilian fauna to the cow-pox virus. The following specimens were submitted to experiences: Procyon cancrivorus, Hydrochoerus capybara, Cavea aperea, Coendu villosus, Didelphis aurita, Bradypus tridactylus, Euphractus sexcintus, Tamandua tetradactylus, Nasua narica, Dasyprocta aguti and Testudo tabulata. In all these animals, - excepting Bradypus tridactylus - we have obtained an infection with incubation (five days, aspect and duration similar to cow-pox of the laboratorial animals (calf and rabbit. In the Bradypus tridactylus howewer, the incubation was very long. Only after 30 days of inoculation we verified the infection with the formation of vesiculae and postulae.

  4. Man-induced hydrological changes, metazooplankton communities and invasive species in the Berre Lagoon (Mediterranean Sea, France).

    Science.gov (United States)

    Delpy, Floriane; Pagano, Marc; Blanchot, Jean; Carlotti, François; Thibault-Botha, Delphine

    2012-09-01

    The Berre Lagoon has been under strong anthropogenic pressure since the early 1950s. The opening of the hydroelectric EDF power plant in 1966 led to large salinity drops. The zooplankton community was mainly composed of two common brackish species: Acartia tonsa and Brachionus plicatilis. Since 2006, European litigation has strongly constrained the input of freshwater, maintaining the salinity above 15. A study was performed between 2008 and 2010 to evaluate how these modifications have impacted the zooplankton community. Our results show that the community is more diverse and contains several coastal marine species (i.e., Centropages typicus, Paracalanus parvus and Acartia clausi). A. tonsa is still present but is less abundant, whereas B. plicatilis has completely disappeared. Strong predatory marine species, such as chaetognaths, the large conspicuous autochtonous jellyfish Aurelia aurita and the invasive ctenophore Mnemiopsis leidyi, are now very common as either seasonal or permanent features of the lagoon. Copyright © 2012 Elsevier Ltd. All rights reserved.

  5. 中西医结合治疗海月水母蜇伤的临床研究

    Institute of Scientific and Technical Information of China (English)

    曹秀丽; 邵月竹

    2001-01-01

    @@ 海月水母Aurelia aurita(Linne,1758)又称水母、海蜇,属腔肠动物门,生活在海水中,呈伞状,直径约几厘米至十厘米,有触须.每年6~10月是水母螫伤的发病季节.近年来,致严重危害或死亡者屡见不鲜,但未引起重视,至今国内外未见报道.我院1997~1999年中西医结合救治取得显著疗效,分析报告如下.

  6. 海月水母培育初报

    Institute of Scientific and Technical Information of China (English)

    陈介康

    1986-01-01

    海月水母(Aurelia aurita)是广泛分布于世界各海区的一种大型水母,成体伞径一般为100—200毫米。国内外生物学教科书中,几乎均以海月水母作为腔肠动物门(Coelenterata)、钵水母纲(Scyphozoa)的代表。它是生物学教学和科研的良好实验材料,也可作为水簇馆的观赏动物。国内尚未见有对海月水母培育的报道。有关大专院校和科研单位一般都无这种水母的无性世代标本。

  7. Symmetrization in jellyfish: reorganization to regain function, and not lost parts.

    Science.gov (United States)

    Abrams, Michael J; Goentoro, Lea

    2016-02-01

    We recently reported a previously unidentified strategy of self-repair in the moon jellyfish Aurelia aurita. Rather than regenerating lost parts, juvenile Aurelia reorganize remaining parts to regain essential body symmetry. This process that we called symmetrization is rapid and frequent, and is not driven by cell proliferation or cell death. Instead, the swimming machinery generates mechanical forces that drive symmetrization. We found evidence for symmetrization across three other species of jellyfish (Chrysaora pacifica, Mastigias sp., and Cotylorhiza tuberculata). We propose reorganization to regain function without recovery of initial morphology as a potentially broad class of self-repair strategy beyond radially symmetrical animals, and discuss the implications of this finding on the evolution of self-repair strategies in animals.

  8. Ephyra jellyfish as a new model for ecotoxicological bioassays.

    Science.gov (United States)

    Faimali, M; Garaventa, F; Piazza, V; Costa, E; Greco, G; Mazzola, V; Beltrandi, M; Bongiovanni, E; Lavorano, S; Gnone, G

    2014-02-01

    The aim of this study was a preliminary investigation on the possibility of using the ephyra of Scyphozoan jellyfish Aurelia aurita (Linnaeus, 1758), the common moon jellyfish, as an innovative model organism in marine ecotoxicology. A series of sequential experiments have been carried out in laboratory in order to investigate the influence of different culturing and methodological parameters (temperature, photoperiod, ephyrae density and age) on behavioural end-points (% of Frequency of Pulsations) and standardize a testing protocol. After that, the organisms have been exposed to two well known reference toxic compounds (Cadmium Nitrate and SDS) in order to analyse the acute and behavioural responses during static exposure. Results of this work indicate that the proposed behavioural end-point, frequency of pulsations (Fp), is an easily measurable one and can be used coupled with an acute one (immobilization) and that ephyrae of jellyfish are very promising model organisms for ecotoxicological investigation.

  9. Predation of three juvenile scyphomedusa species toParalichthys olivaceus and Sebastes schlegelilarvae%三种大型钵水母幼体对牙鲆和许氏平鲉仔稚鱼的捕食选择性

    Institute of Scientific and Technical Information of China (English)

    刘春胜; 庄志猛; 陈四清; 燕敬平; 刘长琳; 陈昭廷

    2016-01-01

    Jellyfish bloom has had numerous deleterious effects in the marine ecosystem worldwide in past decades. In particular, jellyfish bloom impedes the recovery of fish stocks by preying on fish eggs and larvae, and competing strongly for food with fish. Recently, studies have focused on the predatory relationship between fish and jellyfish. However, the species involved vary among different jellyfish-bloom areas.Aurelia aurita,Rhopilema esculentum and Nemopilema nomuraiare, the three common jellyfish species along Chinese coastal seas, of whichA. auritais a cos-mopolitan jellyfish species, and the other two species were indigenous. To the best of our knowledge, the predatory ability of different jellyfish species has not been estimated, as well as the predation of jellyfish on larger fish larvae (more than 10 mm). In this study, the predation of three common jellyfish species (A. aurita,R. esculentum andN. no-murai), as 4-cm medusae, in Chinese coastal seas on fish larvae ofParalichthys olivaceus and Sebastes schlegeli, was studied using the clearance method. All three jellyfish species could prey on fish larvae, and their predatory abilities decreased as the fish grew. The ranges ofA. auritato P. olivaceus and S. schlegeli were not bigger than 15 mm and 20 mm, respectively; the ranges ofR. esculentumto P. olivaceus and S. schlegeli were not bigger than 20 mm and 30 mm, respectively; and the ranges ofN. nomuraito S. schlegeli were not bigger 30 mm (the experiment ofN. nomurai toP. olivaceuswas not done). Clearance rates were similar between the different prey concentrations, and metamorphosis of P. olivaceushelped to avoid predation. The clearance rates of different jellyfish species mainly depended on their vital-ity, though body structure and toxicity of jellyfish were also important factors. Accordingly,R. esculentum had the highest vitality and predatory ability toward fish larvae, followed byN. nomurai andA. aurita. The results provide theoretical evidence of the

  10. A fluid mechanical model for mixing in a plankton predator-prey system

    Science.gov (United States)

    Peng, J.; Dabiri, J. O.

    2009-04-01

    A Lagrangian method is developed to study mixing of small particles in open flows. Particle Lagrangian Coherent Structures (pLCS) are identified as transport barriers in the dynamical systems of particles. We apply this method to a planktonic predator-prey system in which moon jellyfish Aurelia aurita uses its body motion to generate fluid currents which carry their prey to the vicinity of their capture appendages. With the flow generated by the jellyfish experimentally measured and the dynamics of prey particles in the flow described by a modified Maxey-Riley equation, we use pLCS to identify the capture region in which prey can be captured. The properties of the capture region enable analysis of the effects of several physiological and mechanical parameters on the predator-prey interaction, such as prey size, escape force, predator perception, etc. The method provides a new methodology to study dynamics and mixing of small organisms in general.

  11. Evaluation of the trace elements and the total mercury concentration in fishes commercialized at the Cubatao city, Sao Paulo, Brazil; Avaliavao da concentracao de elementos traco e de mercurio total em pescados comercializados na cidade de Cubatao, Estado de Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Fonseca, Barbara C.; Farias, Luciana A.; Curcho, Michel R.M.; Favaro, Debora I.T., E-mail: ba_cortopassi@hotmail.co [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Braga, Elisabete S., E-mail: edsbraga@usp.b [Universidade de Sao Paulo (LABNUT/USP), SP (Brazil). Inst. Oceanografico. Lab. de Nutrientes, Macronutrientes e Tracos no Oceano

    2009-07-01

    This paper evaluates the total Hg and the trace elements As, Br, Co, Cr and Rb concentrations in muscle of commercially important fishes at the Cubatao, Sao Paulo, Brazil, region. The following carnivore species were analysed: jew fish (Micropogonias furnieri), girl leg (Menticirrhus americanus), hake (Macrodon ancylodon), and plant eaters, sardine (Sardella braziliensis) and grey mullet (Mugil liza), representing a total of 58 samples. The analysed trace elements were determined through the neutron analysis activation (NAA) and total Hg, through the atomic absorption spectrometry with cold vapor generation (CV AAS). The analysed elements present a great concentration variation, not only among individuals of the same specie, but also among all the analysed species. The total Hg concentration were highly significant, with the predator species jew fish, girl leg and hake presenting concentrations larger than the non predator species sardine and grey mullet. Nevertheless, the content of total Hg remained bellow the limits established by the Brazilian legislation which is the 500 {mu}g kg{sup -1} for the non predator species, and the 1000 {mu}g{sup -1} for the predator species (humid weight)

  12. A real-time PCR assay to estimate invertebrate and fish predation on anchovy eggs in the Bay of Biscay

    KAUST Repository

    Albaina, A.

    2015-02-01

    In order to investigate the role of predation on eggs and larvae in the recruitment of anchovy (Engraulis encrasicolus), sardine (Sardina pilchardus), sprat (Sprattus sprattus) and 52 macrozooplankton taxa were assayed for anchovy remains in the gut during the 2010 spawning season using a molecular method. This real-time PCR based assay was capable of detecting 0.005. ng of anchovy DNA (roughly 1/100 of a single egg assay) in a reliable way and allowed detecting predation events up to 6. h after ingestion by small zooplankton taxa. A total of 1069 macrozooplankton individuals, 237 sardines and 213 sprats were tested. Both fish species and 32 macrozooplankton taxa showed remains of anchovy DNA within their stomach contents. The two main findings are (1) that the previously neglected macrozooplankton impact in anchovy eggs/larvae mortality is in the same order of magnitude of that due to planktivorous fishes and that, (2) the predation pressure was notably different in the two main spawning centers of Bay of Biscay anchovy. While relatively low mortality rates were recorded at the shelf-break spawning center, a higher predation pressure from both fish and macrozooplankton was exerted at the shelf one.

  13. PROTEKSI MINYAK IKAN LEMURU, MINYAK KELAPA SAWIT, DAN BUNGKIL SAWIT TERHADAP pH DAN NH3DALAM RUMEN SAPI PERANAKAN ONGOLE

    Directory of Open Access Journals (Sweden)

    Catur Suci Purwati

    2016-03-01

    Full Text Available The aimed of this research is to know the influence of protection lemuru oil, palm oil, and palm oil cake to pH and NH3in the rumen of the Ongole. The material were used in this study werecomulated rumenfemale cows with average body weight of 289.33 ± 28.34 kg as many as 3 heads. Latin square experiment design was applied on 3 treatments. Fermented rice straw (FRS, basal concentrate (BC, and protected materials of Indian sardine oil (ISO, palm oil (PO, and palmkernel cake (PKC were used as a feed ingredient.  Treatments were: P1 = FRS 40% + BC 60% (BC 95% + PO 5%; P2 = FRS 40% + BC 60% (BC 95% + ISO 5% ; P3 = FRS 40% + BC 60% (BC 90% + PKC 10%. Parameters measured were pH and NH3. Latin square experiment design was applied on 3 treatments.Conclusions of this study are pH and NH3remain stable, meaning lemuru addition of fish oil, palm oil, and palm oil cake is protected not disturb the digestive process in the cow rumen fistulated onggole breedparticular. (Key word: Indian sardine oil, NH3, Palm kernel cake, Palm oil, pH, Protection

  14. Diversity and synanthropy of Calliphoridae (Diptera) in the region of Rio Claro, SP, Brazil.

    Science.gov (United States)

    De Souza, C R; Zuben, C J V

    2012-06-01

    Dipteran blowflies (Calliphoridae) are of great medical and hygienic importance as vectors of pathogens and as parasites of living and dead tissue, and their association with carrion allows their use in forensic entomology. The objective of this study was to determine the synanthropic index of adult Calliphoridae (Diptera) collected in Rio Claro, São Paulo. Sampling occurred between September 2009 and August 2010. Traps baited with sardines, beef liver, and minced meat were assessed for five consecutive days per month in three distinct ecological areas representing urban, rural, and forest environments. The most abundant species was Lucilia eximia (Wiedemann), followed by Chrysomya albiceps (Wiedemann) and Chrysomya megacephala (Fabricius). Lucilia eximia was the only species present in all seasons and the only species collected during the winter. The season with the lowest abundance was winter, with 69 (5.5%) specimens, and spring was the season with the greatest number of specimens collected (774-61.8%). The only species found outside inhabited areas (synanthropic) was Lucilia cuprina (Wiedemann), with a synanthropy index (SI) value of +5.7. The SI values for the other species were negative, showing a preference for uninhabited areas. The rural and urban areas were most similar in terms of species composition as were the beef and sardine baits. Among the baits used, liver attracted the greatest abundance of calliphorids, whereas minced meat attracted the greatest diversity.

  15. Intraguild predation between small pelagic fish in the Bay of Biscay: impact on anchovy (Engraulis encrasicolus L.) egg mortality

    KAUST Repository

    Bachiller, Eneko

    2015-05-12

    Small pelagic fish can play an important role in various ecosystems linking lower and upper trophic levels. Among the factor behind the observed inter-annual variations in small pelagic fish abundance, intra- and inter-specific trophic interactions could have a strong impact on the recruitment variability (e.g. anchovy). Egg cannibalism observed in anchovies has been postulated to be a mechanism that determines the upper limit of the population density and self-regulates the population abundance of the species. On the other hand, predation by other guild species is commonly considered as a regulation mechanism between competing species. This study provides empirical evidence of anchovy cannibalism and predation of the main small pelagic fish species on anchovy eggs and estimates the effect of intraguild predation on the anchovy egg mortality rate. Results show that, depending on the year (2008–2009), up to 33 % of the total anchovy egg mortality was the result of sardine predation and up to 4 % was the result of egg cannibalism together with predation by Atlantic and Atlantic Chub mackerel and sprat. Results also indicate that in the Bay of Biscay, fluctuations in the survival index of the early life stages of anchovy are likely to be attributable at least in part to egg cannibalism and especially to a high sardine predation on anchovy eggs. © 2015 Springer-Verlag Berlin Heidelberg

  16. Validation of the Biofish-300 HIS Enzymatic Biosensor for the Detection of Histamine in Fishery Products.

    Science.gov (United States)

    Salleres, Sandra; González, Irune; Arantzamendi, Alai; González, Roberto; Maza, Sonia; Jaureguibeitia, Arrate; Hungerford, James M; DeWitt, Christina A; Benner, Ronald A

    2016-09-01

    The Biofish-300 HIS method is a simple, reliable, and specific enzymatic biosensor for the detection of histamine. This technology is highly specific and selective and allows quantification of histamine in fishery products (fresh/frozen and processed) in a short time frame (2-3 min). Histamine in raw tuna, raw mackerel, raw sardine, raw anchovy, boiled tuna, canned tuna in water, canned tuna in oil, canned mackerel in tomato sauce, canned pickled sardine, and canned salted anchovy was analyzed using a water-based extract. Matrix-specific assay procedures and calibration curves were used to enable analyses to be carried out across multiple sample types. The performance of this assay was examined using samples that were naturally contaminated (reference materials and interlaboratory studies) and spiked with histamine. All data were judged against previously established acceptance criteria. Performance measures were evaluated for linearity, selectivity, matrix, lot consistency, and robustness. Results produced in all performance measures, except robustness, were within acceptable ranges. Out-of-range robustness results reflected deviation in sample volume compared to the standard assay procedures. Positive interferences from the presence of agmatine were shown.

  17. Metal concentrations in selected brands of canned fish in Nigeria: estimation of dietary intakes and target hazard quotients.

    Science.gov (United States)

    Iwegbue, Chukwujindu M A

    2015-03-01

    The concentrations of metals (Cd, Pb, Ni, Cr, Cu, Co, Fe, Mn, and Zn) were determined in selected brands of canned mackerel, sardine, and tuna in Nigeria with a view to providing information on the dietary intakes of metals and lifelong health hazards associated with the consumption of these products. The concentrations of metals were determined by using atomic absorption spectrometry after acid digestion. The mean concentrations of metals in canned mackerel, sardine, and tuna were found as 0.04-0.58, 0.06-0.44, 0.32-0.83 μg/g for Cd; 0.05-2.82, 0.70-2.98, 0.23-2.56 μg/g for Pb, 1.33-11.33, canned fish were above their permissible limits while other metals occurred at levels below their permissible limits. The estimated daily intakes of metals from consumption of 20.8 g fish per day by a 60 kg body weight adult were below the provisional tolerable daily intakes for Cd, Pb, Ni, Cr, and Cu and recommended daily intakes for Co, Fe, Mn, and Zn. The estimated target hazard quotients of the examined metals were less than 1 in the majority of the samples indicating no long-term health hazard at the present circumstance.

  18. Heavy metal residues in canned fishes in Egypt.

    Science.gov (United States)

    Morshdy, Alaa Eldin M A; Hafez, Abd-El Salam E; Darwish, Wageh Sobhy; Hussein, Mohamed A M; Tharwat, Ahmed E

    2013-02-01

    A total of 75 random canned fish samples, 25 each of canned (canned tuna, sardine and mackerel) during 2009, were collected from Zagazig Markets for determination of lead, cadmium, zinc, copper and tin residues using atomic absorption spectrophotometer. The obtained results revealed that the mean values of the lead residues in the examined canned tuna, sardine and mackerel were 0.127 +/- 0.02, 0.013 +/- 0.004 and 0.023 +/- 0.01 (ppm) respectively. The mean concentrations of cadmium residues were 0.022 +/- 0.001, 0.048 +/- 0.003 and 0.027 +/- 0.003 ppm, respectively. While in case of zinc, the residual levels were 1.97 +/- 0.12, 2.369 +/- 0.32 and 1.126 +/- 0.24 ppm, respectively. Copper residual levels in the examined samples were 0.293 +/- 0.08, 0.221 +/- 0.03 and 0.08 +/- 0.02 ppm, respectively. In case of tin, the residual levels were 1.496 +/- 0.30, 1.209 +/- 0.26 and 0.379 +/- 0.11 ppm respectively.

  19. Survey into the seafood consumption preferences and patterns in the portuguese population. Gender and regional variability.

    Science.gov (United States)

    Cardoso, Carlos; Lourenço, Helena; Costa, Sara; Gonçalves, Susana; Nunes, Maria Leonor

    2013-05-01

    With the purpose of achieving a deeper knowledge of one of the most important seafood markets in Europe, a survey into the seafood consumption preferences and patterns in the Portuguese population was carried out. A thorough, comprehensive, and simple questionnaire was developed. Consumers were asked to state their preferences towards fish products, their consumption frequencies, the average meal portion, and the usual culinary treatments. Respondents provided personal data: gender, age, geographical location, education level, weight, height, and health condition. This paper presents the first part of the study's results, focusing mainly on the gender and regional variables. Portuguese consumers prefer wild to cultured fish as well as fat to lean fish. Chilled fish is preferred over frozen, salted/dried, canned, and smoked fish, being the latter the least preferred. Soaked cod, hake, and canned tuna are the most eaten seafood products. Men prefer to a greater extent wild and smoked fish. Men consume more cephalopods and sardine and women eat more frequently hake, pink cusk-eel, and redfish. Coastal populations prefer wild fish. Algarve (southern Portugal) consumers exhibit a stronger tendency to wild and whole fish and consume more sardine and sole. Madeira archipelago consumers are particularly fond of black scabbard fish.

  20. Human exposure to PCDD/Fs and PCBs through consumption of fish and seafood in Catalonia (Spain): Temporal trend.

    Science.gov (United States)

    Perelló, Gemma; Díaz-Ferrero, Jordi; Llobet, Juan M; Castell, Victòria; Vicente, Emilio; Nadal, Martí; Domingo, José L

    2015-07-01

    The concentrations of PCDD/Fs and 18 PCBs (DL- and NDL-) were analyzed in 16 fish and seafood species widely consumed in Catalonia (Spain). The exposure of these pollutants was subsequently estimated according to various groups of population. The concentrations of PCDD/Fs and PCBs showed an important decrease in relation to the baseline study (2000) and our last survey (2008). Sardine and red mullet were the species showing the highest pollutant concentrations, while canned tuna and cuttlefish presented the lowest levels. Sardine was the main contributor to the exposure of PCDD/Fs and PCBs. In contrast, swordfish was the species with the lowest contribution to the exposure of PCDD/Fs, DL-PCBs, and PCDD/Fs+DL-PCBs, while clam was the minor contributor for NDL-PCBs and total PCBs. For all groups of population, the current intakes of PCDD/Fs and PCBs were lower than the TDI (1-4 pg WHO-TEQ/kg body weight/day), being children the group with the highest exposure. However, this exposure should not mean a health risk for this group of population. The current intake of PCDD/Fs and PCBs through fish and seafood consumption was similar or even lower than most values reported in recent studies all over the world.

  1. What is the effect of soil use on ant communities?

    Science.gov (United States)

    Schmidt, Fernando A; Diehl, Elena

    2008-01-01

    Studies on ant communities in agroecosystems have contributed to the knowledge of the effect of agricultural activities on biological communities. The aim of this study is to explain the effect of soil use on ant communities. We tested the hypothesis that there was a decrease in ant species richness and a change in the species composition at habitats with more intense soil use. We collected ants using sardine baits, subterranean traps and direct sampling at four habitats with different soil use (secundary forest, Acacia forestry, initial stage of succession and mixed crops). The ant species richness did not decrease with intensity of soil use. In successional habitat the species numbers collected using sardine baits and subterranean traps were significantly different. Species composition of communities had a pronounced variation, with the epigaeic and hypogaeic ant faunas of the habitat with high intense soil use (mixed crops) had low similarity with ant communities of the three other habitats. The predator species were restricted to habitats with low intensity of soil use. Then, species composition could better reflect the functional changes on ant communities than species richness. Our data can help to choose the component of ant community that better reflect the response of biodiversity to agricultural impacts.

  2. Feeding habits of European pilchard late larvae in a nursery area in the Adriatic Sea

    Science.gov (United States)

    Borme, Diego; Tirelli, Valentina; Palomera, Isabel

    2013-04-01

    European pilchard Sardina pilchardus late larvae were collected in the Gulf of Manfredonia, an important nursery area, during their seasonal inshore occurrence. Thanks to diel cycle sampling and to the wide range of larval lengths (from a minimum of 27 mm to a maximum of 45 mm), both feeding rhythm and ontogenetic changes were analysed. The feeding peak was observed in the afternoon, before sunset. Sardine larvae were exclusively zooplanktivorous, their diet being based on Calanoid Copepods from the genus Paracalanus (IRI% = 65.7), on the species Temora longicornis (IRI% = 15.5) and other small-sized Copepods. Other planktonic organisms appeared in the stomach contents occasionally and never reached IRI% values > 1. The number of prey per stomach increased suddenly at larval lengths around 40 mm, corresponding to the development of the stomach. Prey composition in the environment was established by contemporaneous sampling of plankton, performed by means of two plankton nets with different meshes. The main prey items were positively selected among those available in the field, but some other prey (Centropages spp., Harpacticoids, Corycaeids, Temora stylifera and Acartia spp.) were also preferred, although rare in the plankton samples. In contrast, copepod nauplii, despite their abundance in the environment (15,848 ± 4441 individuals m- 3), were only occasionally recovered in the larval gut contents (N = 0.26%). This shows that sardine late larvae have switched to larger prey items.

  3. Characterization of triacylglycerol composition of fish oils by using chromatographic techniques.

    Science.gov (United States)

    Solaesa, Ángela García; Bucio, Silvia Liliana; Sanz, María Teresa; Beltrán, Sagrario; Rebolleda, Sara

    2014-01-01

    Triacylglycerols (TAG) of two different refined fish oils from sardine and a mixture of tuna and sardine oil were separated by reverse phase high performance liquid chromatography (RP-HPLC) with a binary solvent gradient of acetone/acetonitrile. Different fractions were observed in the chromatogram and TAG species were tentatively identified by subsequent analysis of the fatty acid (FA) profile in each fraction by capillary Gas Chromatography (GC). Peak identities were assigned on the basis of a multiple linear regression analysis by using factors such as carbon number, number of double bonds, number of monounsaturated fatty acids (MUFA) and number of polyunsaturated fatty acids (PUFA) in the molecule as predictors for TAG retention time. A successful correlation was obtained between retention times and the equivalent carbon number (ECN) of triacylglycerols. Regiospecific analysis of fatty acids in the TAG has been conducted by ethanolysis of the fish oil by using an immobilized lipase. The subsequent separation of 2-monoacylglycerol (2-MAG) by TLC (thin layer chromatography) analysis showed that ethanolysis system is effective for analysis of FA composition at the 2-position in oils containing PUFA. Principal components analysis (PCA) has been also applied to establish correlations between the different fatty acids in the TAG.

  4. Exposure to PBDEs and PCDEs associated with the consumption of edible marine species.

    Science.gov (United States)

    Domingo, Jose L; Bocio, Ana; Falcó, Gemma; Llobett, Juan M

    2006-07-15

    In the present study, the concentrations of PBDEs and PCDEs were determined in 14 edible marine species widely consumed by the population of Catalonia (Spain). The daily intake of PBDEs and PCDEs associated with this consumption was also determined. A total of 42 composite samples were analyzed by HRGC/HRMS. The highest PBDE levels (ng/kg wet weight) were found in salmon (2015) followed by mackerel, swordfish, and red mullet (1124, 978, and 769, respectively), while those of PCDEs (ng/kg wet weight) were detected in red mullet (7088) followed by sardine (1829), anchovy (1606), tuna (1292), and mackerel (1031). For a standard male adult, total PBDE and PCDE intakes through edible marine species were 20.8 and 39.4 ng/ day, respectively. The highest contributions to these intakes (ng/day) corresponded to the consumption of tuna (5.7), salmon (3.6), and hake (3.5) for PBDEs, and tuna (13.1), hake (7.3), and sardine (6.9) for PCDEs. Although currently there is not evidence of the dioxin-like behavior of PBDEs, further research is necessary to assess if long-term exposure to PBDEs, mainly through the diet, may mean adverse effects to humans. With respectto PCDE congeners, to establish TEF values would be of great value to evaluate human health risks.

  5. Effect of smoke generation sources and smoke curing duration on the levels of polycyclic aromatic hydrocarbon (PAH) in different suites of fish.

    Science.gov (United States)

    Essumang, D K; Dodoo, D K; Adjei, J K

    2013-08-01

    The research studied the impact of smoke generation sources on PAH contamination in four different smoke-cured fish (mackerel, sardine, tuna and Cigar minnows). The smoke sources used included acacia, sugarcane bagasse and mangrove. PAHs in the smoke-cured fish were analysed using Varian GC/MS (3800-GC) system. The mean total PAH concentrations in the smoked fish (n=108) ranged from 250.59-1376.09 μg/kg in tuna, cigar minnows, sardine and mackerel smoke-cured with sugarcane bagasse, mangrove and acacia for between 2 and 8h. The mean BaP levels for most fish cured with smoke from acacia and mangrove for between 2 and 8h were all above the European Commission set limit of 5.0 μg/kg. Positive correlations (at P=0.01, 2-tailed) were observed between PAH levels in smoked fish and lignin contents of wood type used for the smoke generation, the fat content and the smoke-curing duration. Risk assessment conducted using benzo[a]pyrene carcinogenic and mutagenic toxicity equivalency factors (TEF and MEF respectively) showed high risk associated with consuming fish smoke-cured with hard woods (acacia and mangroves). Sugarcane bagasse was found to be relatively the best and safest smoke-generating source for smoke-curing of fish among the three wood types when using the traditional kiln. Copyright © 2013 Elsevier Ltd. All rights reserved.

  6. Chronic lack of breeding by Galápagos Blue-footed Boobies and associated population decline

    Directory of Open Access Journals (Sweden)

    David Anchundia

    2014-06-01

    Full Text Available A survey of Blue-footed Boobies (Sula nebouxii excisa throughout the taxon's range in Galápagos, Ecuador found ~6400 adults, compared to a rough estimate of 20,000 in the 1960s. Few pairs bred in 2011-2013 and almost no birds in juvenile plumage were seen. Long-term data suggest that poor breeding began in 1998. Lack of recruitment over this period would mean that the current population is mostly elderly and experiencing senescent decline in performance. Anthropogenic effects such as introduced predators are unlikely to explain this decline because islands with and without such factors exhibited the same low breeding. The poor reproduction seems to be linked to diet. Previous work indicated that sardine and herring (Clupeidae supported successful breeding, but these fish were mostly absent from the diet during this study, except in the central part of Galápagos, where most breeding attempts during this study occurred. Elsewhere in the eastern Pacific sardine abundance has decreased dramatically by natural processes in the last 15 years, as part of a well-documented and apparently natural cycle. This cyclic change in abundance provides a possible explanation for the recent demographic changes in Blue-footed Boobies in Galápagos. Whether natural or anthropogenic in origin, the implications of senescent decline in breeding ability and survival are dramatic for this genetically distinct icon of biodiversity and ecotourism.

  7. Arsenic speciation in fish from Greek coastal areas.

    Science.gov (United States)

    Kalantzi, Ioanna; Mylona, Kyriaki; Sofoulaki, Katerina; Tsapakis, Manolis; Pergantis, Spiros A

    2017-06-01

    Arsenic speciation analysis was conducted on fish samples (sardine and anchovy) collected from six areas along the Greek coastline, i.e. Artemisium Straits, Thermaikos Gulf, Amvrakikos Gulf, Strymonian Gulf, Thracian Sea, and Elefsina Gulf. Total arsenic levels ranging from 11.8 to 62.6mg As/kg dry weight were determined. Arsenobetaine, a non-toxic form of arsenic, was found to be the main arsenic species, present at 8.6 to 58.8mg As/kg dry weight, accounting for 67-95% of the total arsenic. Also detected in all fish samples was dimethylarsinic acid, although at considerably lower concentrations, ranging from 0.072-0.956mg As/kg dry weight. Monomethylarsonic acid was detected at low levels in all anchovy samples, and only in sardines from one area. Finally, inorganic arsenic in the form of arsenate was detected only in fish at one area, indicating the possible effect of an environmental parameter on its presence at detectable amounts. Statistical analysis revealed the environmental variables, such as salinity, total organic carbon and nitrogen, ammonium, phosphate, total phosphorus, dissolved oxygen and pressure index, are potentially correlated to As species concentrations. Furthermore, based on factor analysis, the biological parameters, such as fish weight, lipids, protein and ash content, that are correlated to As species concentrations of fish were also identified. The interrelationship of arsenobetaine and dimethylarsinic acid concentrations within each fish species was evaluated. Copyright © 2017. Published by Elsevier B.V.

  8. Influence of ocean winds on the pelagic ecosystem in upwelling regions.

    Science.gov (United States)

    Rykaczewski, Ryan R; Checkley, David M

    2008-02-12

    Upwelling of nutrient-rich, subsurface water sustains high productivity in the ocean's eastern boundary currents. These ecosystems support a rate of fish harvest nearly 100 times the global mean and account for >20% of the world's marine fish catch. Environmental variability is thought to be the major cause of the decadal-scale biomass fluctuations characteristic of fish populations in these regions, but the mechanisms relating atmospheric physics to fish production remain unexplained. Two atmospheric conditions induce different types of upwelling in these ecosystems: coastal, alongshore wind stress, resulting in rapid upwelling (with high vertical velocity, w); and wind-stress curl, resulting in slower upwelling (low w). We show that the level of wind-stress curl has increased and that production of Pacific sardine (Sardinops sagax) varies with wind-stress curl over the past six decades. The extent of isopycnal shoaling, nutricline depth, and chlorophyll concentration in the upper ocean also correlate positively with wind-stress curl. The size structure of plankton assemblages is related to the rate of wind-forced upwelling, and sardine feed efficiently on small plankters generated by slow upwelling. Upwelling rate is a fundamental determinant of the biological structure and production in coastal pelagic ecosystems, and future changes in the magnitude and spatial gradient of wind stress may have important and differing effects on these ecosystems. Understanding of the biological mechanisms relating fisheries production to environmental variability is essential for wise management of marine resources under a changing climate.

  9. Development and validation of an ion-pair chromatographic method for simultaneous determination of trans- and cis-urocanic acid in fish samples.

    Science.gov (United States)

    Zare, Davood; Muhammad, Kharidah; Bejo, Mohd Hair Bin; Ghazali, Hasanah Mohd

    2012-09-21

    Urocanic acid (UCA) has been reported to be a mast cell degranulator and has also been suggested as a complementary agent in implicated scombroid fish poisoning. In this research, a new method is described to extract, clean up and perform simultaneous ion-pair chromatographic analysis of trans- and cis-urocanic acid (UCA) in fish samples. UCA was extracted using 0.05 M HCl and protein was removed from the extract by precipitation with 10% trisodium citrate and 10% citric acid. The HPLC method that is developed showed a rapid, precise and sensitive method with short retention time for simultaneous separation of UCA isomers in fish samples. Estimation of trans- and cis-UCA in the muscle of Indian mackerel, tuna and sardine showed that, as expected, no cis-UCA existed in fish muscles and the highest concentration of trans-UCA was found in Indian mackerel with 118.8 mg kg(-1) while the highest concentrations of trans-UCA in tuna and sardine were 12.1 and 17.5 mg kg(-1), respectively. Copyright © 2012 Elsevier B.V. All rights reserved.

  10. Born small, die young: Intrinsic, size-selective mortality in marine larval fish

    KAUST Repository

    Garrido, S.

    2015-11-24

    Mortality during the early stages is a major cause of the natural variations in the size and recruitment strength of marine fish populations. In this study, the relation between the size-at-hatch and early survival was assessed using laboratory experiments and on field-caught larvae of the European sardine (Sardina pilchardus). Larval size-at-hatch was not related to the egg size but was significantly, positively related to the diameter of the otolith-at-hatch. Otolith diameter-at-hatch was also significantly correlated with survival-at-age in fed and unfed larvae in the laboratory. For sardine larvae collected in the Bay of Biscay during the spring of 2008, otolith radius-at-hatch was also significantly related to viability. Larval mortality has frequently been related to adverse environmental conditions and intrinsic factors affecting feeding ability and vulnerability to predators. Our study offers evidence indicating that a significant portion of fish mortality occurs during the endogenous (yolk) and mixed (yolk /prey) feeding period in the absence of predators, revealing that marine fish with high fecundity, such as small pelagics, can spawn a relatively large amount of eggs resulting in small larvae with no chances to survive. Our findings help to better understand the mass mortalities occurring at early stages of marine fish.

  11. Semantic involvement of initial and final lexical embeddings during sense-making: The advantage of starting late

    Directory of Open Access Journals (Sweden)

    Petra M. Van Alphen

    2012-06-01

    Full Text Available During spoken language interpretation, listeners rapidly relate the meaning of each individual word to what has been said before. However, spoken words often contain spurious other words, like day in daisy, or dean in sardine. Do listeners also relate the meaning of such unintended, spurious words to the prior context? We used ERPs to look for transient meaning-based N400 effects in sentences that were completely plausible at the level of words intended by the speaker, but contained an embedded word whose meaning clashed with the context. Although carrier words with an initial embedding (day in daisy did not elicit an embedding-related N400 effect relative to matched control words without embedding, carrier words with a final embedding (dean in sardine did elicit such an effect. Together with prior work from our lab and the results of a Shortlist B simulation, our findings suggest that listeners do semantically interpret embedded words, albeit not under all conditions. We explain the latter by assuming that the sense-making system adjusts its hypothesis for how to interpret the external input at every new syllable, in line with recent ideas of active sampling in perception.

  12. A geometric morphometric analysis of cranial and mandibular shape variation of didelphid marsupials

    Directory of Open Access Journals (Sweden)

    D. Astúa de Moraes

    2000-06-01

    Full Text Available Abstract The New World marsupial family Didelphidae is one of the oldest among mammals and is usually regarded as a morphologically conservative group. We analyzed cranial shape variation among six species of the six largest living genera of the family using two-dimensional landmark data. We captured and digitized video images of the skull and mandible for the following species: Caluromys philander (n = 65, Chironectes minimus (n = 30, Didelphis aurita (n = 70, Lutreolina crassicaudata (n = 37, Metachirus nudicaudatus (n = 77 and Philander frenata (n = 62. Fourteen landmarks were defined for the lateral, 25 for the ventral, 23 for the dorsal views of the skull, and nine on the mandibular lateral view. Sex, species, and interaction effects were analyzed with a two-way MANOVA on the matrices of coordinates aligned by general least squares. All four views had significant interactions. Canonical Variates Analysis was performed on sexes and species, and shape was regressed on the canonical variate scores for each species. Caluromys philander was clearly the most distinct species, with paedomorphic features that can be related to its arboreal habits. A conspicuous shortening of the rostrum distinguishes the highly carnivorous Lutreolina crassicaudata. Didelphis aurita and Philander frenata overlapped somewhat, reflecting shape similarities associated with their phylogenetic affinities, while the few differences observed are probably allometric consequences of size differences. Philander frenata and Chironectes minimus showed similar cranial shapes, while Metachirus nudicaudatus was distinctive with a broad and elongated rostrum. In spite of an overall similar shape, the geometric morphometric approach revealed several marked differences among species that can be related to their phylogenetic origin and their adaptive

  13. Ecosystem relevance of variable jellyfish biomass in the Irish Sea between years, regions and water types

    Science.gov (United States)

    Bastian, Thomas; Lilley, Martin K. S.; Beggs, Steven E.; Hays, Graeme C.; Doyle, Thomas K.

    2014-08-01

    Monitoring the abundance and distribution of taxa is essential to assess their contribution to ecosystem processes. For marine taxa that are difficult to study or have long been perceived of little ecological importance, quantitative information is often lacking. This is the case for jellyfish (medusae and other gelatinous plankton). In the present work, 4 years of scyphomedusae by-catch data from the 2007-2010 Irish Sea juvenile gadoid fish survey were analysed with three main objectives: (1) to provide quantitative and spatially-explicit species-specific biomass data, for a region known to have an increasing trend in jellyfish abundance; (2) to investigate whether year-to-year changes in catch-biomass are due to changes in the numbers or in the size of medusa (assessed as the mean mass per individual), and (3) to determine whether inter-annual variation patterns are consistent between species and water masses. Scyphomedusae were present in 97% of samples (N = 306). Their overall annual median catch-biomass ranged from 0.19 to 0.92 g m-3 (or 8.6 to 42.4 g m-2). Aurelia aurita and Cyanea spp. (Cyanea lamarckii and Cyanea capillata) made up 77.7% and 21.5% of the total catch-biomass respectively, but species contributions varied greatly between sub-regions and years. No consistent pattern was detected between the distribution and inter-annual variations of the two genera, and contrasting inter-annual patterns emerged when considering abundance either as biomass or as density. Significantly, A. aurita medusae were heavier in stratified than in mixed waters, which we hypothesize may be linked to differences in timing and yield of primary and secondary productions between water masses. These results show the vulnerability of time-series from bycatch datasets to phenological changes and highlight the importance of taking species- and population-specific distribution patterns into account when integrating jellyfish into ecosystem models.

  14. [Reason to split the genus Aurelia. Mesoglein from two populations differ].

    Science.gov (United States)

    Kotova, A V; Podgornaia, O I; Adonin, L S

    2015-01-01

    The medusa, Aurelia aurita (Scyphozoa, Cnidaria), is considered to be a cosmopolitan species with a worldwide distribution in most seas from the poles to the tropics. Cnidarian is thought to possess two tissue layers: endoderm (gastroderm) and ectoderm, which are separated by huge mesoglea in medusa. The basic morphology of medusa is similar in different populations. Previously we have determined a new protein "mesoglein" as one of the main components of mesoglea. Deduced amino acid sequence of mesoglein contains Zona Pellucida (ZP) domain. In this paper, we have comparied of mesoglein and its gene in medusa from three habitats (White Sea (WsA), Black Sea (BsA), Japonic Sea (JsA)). The set of the mesoglea protein bands after SDS-PAGE is similar in all samples. Nevertheless, JsA mesogleins' M(r) is 53-55 kDa, while WsA and BsA mesogleins have M(r) of 47 kDa. Antibodies raised against WsA mesoglein recognize only mesogleins with M(r) of 47 kDa, but not 53-55 kDa, both on immunoblot and immunocytochemistry. Mesogleal cells and elastic fibrils are stained intensively in the mesoglea both from WsA and BsA but not from JsA. The possibility of gene divergency was checked by PCR with primers specific for WsA mesoglein gene. PCR products of expected length obtained on polyA-cDNA template from mesogleal cells of WsA and BsA medusa but not on cDNA of JsA medusa. Our results evidence that there are two different species in genus Aurelia: Aurelia aurita inhabits White and Black Seas while Aurelia sp. inhabits Japonic Sea. This is consistent with findings of other recept molecular biological studies.

  15. n3- polyunsaturated Fat Acid Content of Some Edible Fish from Bahrain Waters

    Science.gov (United States)

    Al-Arrayedu, F. H.; Al Maskati, H. A.; Abdullah, F. J.

    1999-08-01

    This study was performed to determine the content of n3- polyunsaturated fatty acids in 10 fish species that are commonly consumed in Bahrain in addition to the main commercial shrimp species. White sardinella, which is a plankton feeder, had the highest content of n3- polyunsaturated fatty acids. It had the highest value of eicosapentaenoic acid (146.5 ± 20 mg 100 g-1) and linolenic acid (98.9±f 100 g-1) and the second highest value of docosahexaenoic acid at (133.7 ± 22 mg 100 g-1). Spanish mackerel which feeds mainly on sardinella was second with eicosapentaenoc acid at 55 ± 5.4 mg 100 g-1, docosahexaenoic acid at 161 ± 19.8 mg 100 g-1, linolenic acid at 16.4 mg 100 g-1 and docosapentaenoic acid at 25 ± 1.9 mg 100 g-1. Rabbitfish, the most popular edible fish in Bahrain which feeds mainly on benthic algae had the third highest content of n3- polyunsaturated fatty acids with eicosapentaenoic acid at 37.5 ± 3.9 mg 100 g-1, docosahexaenoic acid at 76 ± 6.7 mg 100 g-1, and docosapentaenoic acid at 85.8 ± 10 mg 100 g-1. The other fish and crustacean species studied were Arabian carpet shark, doublebar bream, grouper, gray grunt, golden travally, keeled mullet, spangled emperor and shrimp. The study explores the transfer of n3- polyunsaturated fatty acids through the food webs of the examined fish. It is apparent, generally, that plankton feeders displayed the highest content of n3- polyunsaturated fatty acids followed by seaweed and algae grazers, with benthic carnivores feeding on invertebrates displaying the poorest content. The values reported here, however, are much lower than those reported for fish available in American markets and in Mediterranean fish. Warm water temperature and high salinity which lead to lowering of the density of phytoplankton and phytoplankton content of n3- polyunsaturated fatty acids are suggested as the reason for the observed low values of n3- polyunsaturated fatty acids in Bahrain fish.

  16. Ocorrências de larvas e jovens de peixes na Ilha Anchieta (SP, com algumas anotações sobre a morfologia da castanha, Umbrina coroides Cuvier, 1830 The occurrence of fish larvae and juveniles in Anchieta Island (SP, with some notes on the morphology of the croaker, Umbrina coroides, 1830

    Directory of Open Access Journals (Sweden)

    Yasunobu Matsuura

    1979-06-01

    Full Text Available Relaciona-se neste trabalho a ocorrência de 11 famílias e 26 gêneros de diferentes espécies de larvas e jovens de peixes coletados na Ilha Anchieta, Ubatuba (Lat. 23º32'S - Long. 45º05'W, durante os anos de 1973 e 1974. Foram analisados todos os peixes coletados, procurando-se estabelecer o período das desovas através da ocorrência dos diferentes estágios de desenvolvimento em que se encontravam, relacionando-os com as condições ambientais. A espécie mais abundante foi Eucinostomus sp., que ocorreu principalmente no verão. Urribrina coroides apareceu quase que durante o ano todo, Menticirrhus littoralis durante o inverno, enquanto que M. americanas ocorreu durante o verão. No outono, houve a ocorrência de Coleotropis sp. e a espécie mais abundante durante o inverno foi Anchoa lyolepis. Outras espécies que ocorreram em abundância durante o verão foram Albula vulpes, Sardinella brasiliensis, Harengula jaguana, Trachinotus carolinus e T. falcatus. Apresenta-se também a descrição morfológica de larvas e jovens de castanha (Umbrina coroides.Occurrences of fish larvae and juveniles taken by beach seine at the Ilha Anchieta (Lat. 23º32'S; Long. 45º05'W during the period from August 1973 to July 1974, were studied. The predominant species was Eucinostomus sp. (Gerreidae which occurred more abundantly during summer season. Other species which occurred during hot summer season were: Albula vulpes (Linnaeus, 1758 (Albulidae, Sardinella brasiliensis (Steindachner, 1879, Harengula Jaguana Poey, 1865 (Clupeidae, Trachinotus carolinus (Linnaeus, 1766, Trachinotus falcatus (Linnaeus, 1758 (Carangidae, and Menticirrhus americanus (Linnaeus, 1758 (Sciaenidae. Menticirrhus littoralis (Holbrook, 1856, occurred all the year-round. Anchoa lyolepis (Evermann & Marsh, 1902 (Engraulidae occurred more abundantly during winter and Coleotropis sp. (Atherinidae during autumn. Morphological description of larvae and juveniles of Umbrina coroides

  17. Elaboração e análise sensorial de produto com fibra solúvel, ômega-3 e isento de lactose. = Development and sensory analysis of a lactose-free food product with soluble dietary fiber and omega-3 fatty acid.

    Directory of Open Access Journals (Sweden)

    Paula Bucharles

    2009-07-01

    Full Text Available Doenças Inflamatórias Intestinais (DII – Colite Ulcerativa e Doença de Crohn são inflamações crônicas no trato gastrintestinal com etiologia indefinida. O decréscimo da ingestão oral, má absorção e perda acelerada de nutrientes requer uma terapia nutricional adequada. Objetivou-se com esse trabalho elaborar uma receita de Torta de Sardinha, com fi bras, ômega-3 e isenta de lactose, bem como avaliar sensorialmente sua aceitabilidade entre portadores e não portadores de doença infl amatória intestinal. Verificou-se o nível de aceitação das amostras usando escala hedônica estruturada de nove pontos. Participaram do teste 61 provadores, sendo 16 portadores de DII e 44 indivíduos não portadores de DII. A torta de sardinha apresentou reduzido teor de gordura total e saturada, não afetando possivelmente nos resultados na análise sensorial do produto. A torta de sardinha teve boa aceitação pela preparação que variou entre os termos hedônicos “gostei muitíssimo” e “gostei muito” (portadores da patologia, e “gostei muito” e “gostei moderadamente” (indivíduos sadios. = Inflammatory Bowel Diseases (IBD - Ulcerative Colitis and Crohn’s disease are chronic inflammation in the gastrointestinal tract with unknown etiology. The decrease in oral intake, malabsorption, and accelerated loss of nutrients require adequate nutritional therapy. Thus, this study aimed to develop a lactose-free recipe of Sardine Pie with fi ber andomega-3 fatty acid. Besides, evaluate the acceptability by healthy individuals and individuals with IBD. 61 panelists participated in the test, 16 patients with IBD and 44 healthy individuals. A 9-point-hedonic scale was used for the test. The data analysis presented no signifi cant difference (p<0.05 between the study groups regarding acceptance. The sardine pie presented low levels of total and saturated fat, not possibly affecting the results in the sensory evaluation of the product. Both

  18. Comparison of the nutritional and toxicological reference values of trace elements in edible marine fish species consumed by the population in Rio De Janeiro State, Brazil

    Directory of Open Access Journals (Sweden)

    Renata Jurema Medeiros

    2014-01-01

    Full Text Available The present study estimated the human daily and weekly intake of inorganic elements due to consumption of fish in Rio de Janeiro state and the associated potential health risks posed by some toxic elements. All samples analyzed had values of Cd and Pb below the Maximum Tolerable Limits of 3.0 mg kg−1 for Pb and 1.0 mg kg−1 for Cd; only Mugil cephalus, Cynoscion leiarchus and Caranx crysos had As concentrations below 1 mg kg−1, maximum limit established by Brazilian legislation. The higher values of Cd and Pb correspond to 0.22% of PTWI and the higher value of As corresponds to 8.6% of PTWI. None of the studied species showed values higher than PTWI. The higher values of Cu EDI found in Pomatomus numida correspond to 33.3% of RDA; Fe in Salmo salar and Genypterus brasiliensis corresponds to 4.3% of EDI; Mn in Sardinella brasiliensis corresponds to 7.4% of EDI; Zn in S. salar corresponds to 13.2% of EDI and Se in S. salar corresponds to 20.6% of EDI. Some species can be a good source of inorganic elements. For risk assessment, it is important to assess specific eating habits of each region to avoid underestimating the data.

  19. Ecosystem structure and trophic analysis of Angolan fishery landings

    Directory of Open Access Journals (Sweden)

    Ronaldo Angelini

    2011-06-01

    Full Text Available Information on the mean trophic level of fishery landings in Angola and the output from a preliminary Ecopath with Ecosim (EwE model were used to examine the dynamics of the Angolan marine ecosystem. Results were compared with the nearby Namibian and South African ecosystems, which share some of the exploited fish populations. The results show that: (i The mean trophic level of Angola’s fish landings has not decreased over the years; (ii There are significant correlations between the landings of Angola, Namibia and South Africa; (iii The ecosystem attributes calculated by the EwE models for the three ecosystems were similar, and the main differences were related to the magnitude of flows and biomass; (iv The similarity among ecosystem trends for Namibia, South Africa and Angola re-emphasizes the need to continue collaborative regional studies on the fish stocks and their ecosystems. To improve the Angolan model it is necessary to gain a better understanding of plankton dynamics because plankton are essential for Sardinella spp. An expanded analysis of the gut contents of the fish species occupying Angola’s coastline is also necessary.

  20. Variation in Nutritive Composition of Two Commercially Important Marine Fin Fishes

    Directory of Open Access Journals (Sweden)

    R. Kanagalakshmi

    2012-01-01

    Full Text Available The chemical compositions of fishes vary and are closely related to feed intake, migratory swimming and sexual changes in connection with spawning. The nutritive composition of many marine, estuarine and fresh water fishes were determined and have been implemented in fish consumption. Nutritive composition of Dussumieria acuta and Sardinella brachysoma were not yet determined and the present study was dealt with these fishes. Fatty acids, Vitamins (fat soluble and water soluble and Minerals were quantified. Palmitic acid showed its higher level in both species (5.78 and 3.89% in D. acuta and S. brachysoma, respectively. Water-soluble vitamins were higher in D. acuta and fat-soluble vitamins showed higher percentage availability in S. brachysoma. Calcium was the predominant mineral in both fishes and can be consumed by humans for bone strength. The finding has proved with strong evidence that both the fishes undertaken for the present study are rich in most of the nutrients essential for proper health maintenance of humans.

  1. DHA and EPA Content and Fatty Acid Profile of 39 Food Fishes from India

    Directory of Open Access Journals (Sweden)

    Bimal Prasanna Mohanty

    2016-01-01

    Full Text Available Docosahexaenoic acid (DHA is the principal constituent of a variety of cells especially the brain neurons and retinal cells and plays important role in fetal brain development, development of motor skills, and visual acuity in infants, lipid metabolism, and cognitive support and along with eicosapentaenoic acid (EPA it plays important role in preventing atherosclerosis, dementia, rheumatoid arthritis, Alzheimer’s disease, and so forth. Being an essential nutrient, it is to be obtained through diet and therefore searching for affordable sources of these ω-3 polyunsaturated fatty acids (PUFA is important for consumer guidance and dietary counseling. Fish is an important source of PUFA and has unique advantage that there are many food fish species available and consumers have a wide choice owing to availability and affordability. The Indian subcontinent harbors a rich fish biodiversity which markedly varies in their nutrient composition. Here we report the DHA and EPA content and fatty acid profile of 39 important food fishes (including finfishes, shellfishes, and edible molluscs from both marine water and freshwater from India. The study showed that fishes Tenualosa ilisha, Sardinella longiceps, Nemipterus japonicus, and Anabas testudineus are rich sources of DHA and EPA. Promotion of these species as DHA rich species would enhance their utility in public health nutrition.

  2. Seasonal variations in diversity and abundance of surface ichthyoplankton in the northern South China Sea

    Institute of Scientific and Technical Information of China (English)

    LI Kaizhi; YIN Jianqiang; HUANG Liangmin; LIN Zhaojin

    2014-01-01

    The objective of this study was to investigate the seasonal variations of ichthyoplankton diversity and abun-dance in the northern South China Sea based on the data collected during summer, winter and spring. In total, 95 taxa of larval fishes were identified. The greatest number of species was recorded in spring, followed by summer and winter. The number of species was distributed mainly in the coastal waters from the east of Leizhou Peninsula to the southeast of Hainan Island during the surveyed periods of summer and spring, but in the offshore waters during winter. The abundance of larval fish was lowest in winter, increased in spring, and reached the maximum in summer. High abundance of larval fish was generally restricted to coastal waters with the isobaths less than 50 m. Seasonal variations of larval fish richness, abundance and diversity index were significant (P<0.001). Carangidae was the most common and abundant taxon in summer and winter, whileSardinella sp.,Thrissa mystax andLeiognathus sp. were dominant in spring. High diversity and abundance of larval fish might be attributed to increased temperature and coastal upwelling in spring and summer.

  3. DHA and EPA Content and Fatty Acid Profile of 39 Food Fishes from India.

    Science.gov (United States)

    Mohanty, Bimal Prasanna; Ganguly, Satabdi; Mahanty, Arabinda; Sankar, T V; Anandan, R; Chakraborty, Kajal; Paul, B N; Sarma, Debajit; Syama Dayal, J; Venkateshwarlu, G; Mathew, Suseela; Asha, K K; Karunakaran, D; Mitra, Tandrima; Chanda, Soumen; Shahi, Neetu; Das, Puspita; Das, Partha; Akhtar, Md Shahbaz; Vijayagopal, P; Sridhar, N

    2016-01-01

    Docosahexaenoic acid (DHA) is the principal constituent of a variety of cells especially the brain neurons and retinal cells and plays important role in fetal brain development, development of motor skills, and visual acuity in infants, lipid metabolism, and cognitive support and along with eicosapentaenoic acid (EPA) it plays important role in preventing atherosclerosis, dementia, rheumatoid arthritis, Alzheimer's disease, and so forth. Being an essential nutrient, it is to be obtained through diet and therefore searching for affordable sources of these ω-3 polyunsaturated fatty acids (PUFA) is important for consumer guidance and dietary counseling. Fish is an important source of PUFA and has unique advantage that there are many food fish species available and consumers have a wide choice owing to availability and affordability. The Indian subcontinent harbors a rich fish biodiversity which markedly varies in their nutrient composition. Here we report the DHA and EPA content and fatty acid profile of 39 important food fishes (including finfishes, shellfishes, and edible molluscs from both marine water and freshwater) from India. The study showed that fishes Tenualosa ilisha, Sardinella longiceps, Nemipterus japonicus, and Anabas testudineus are rich sources of DHA and EPA. Promotion of these species as DHA rich species would enhance their utility in public health nutrition.

  4. 巴黎历险记%Adventure in Paris

    Institute of Scientific and Technical Information of China (English)

    良晨

    2003-01-01

    @@ Part Five:Iron Cage and Attle V.铁笼与阁楼 Before reaching my imagined destinations:the 8th district where the Elysée Palace and the Ma tigon Hotel were situated, the van stopped in front of an old building in the 6th district, near the Church Saint-Germain. It was a police station. Of course there was no red carpet out for me. The waiting hall was an iron cage where there were already plenty of sardines(沙丁鱼) . The cage was so full that my bodyguards had to cram(填满)me into it, boxed in with at least 15 people. It must have had the highest population density( 人口密度 ) of anywhere in the word. Squeezed in among the others, I could hardly breathe and soon was dripping with sweat. The waiting time was long; one minute seemed to me a century.

  5. The Great White Guppy: Top Predator

    Science.gov (United States)

    Michalski, G. M.

    2011-12-01

    Nitrogen isotopes are often used to trace the trophic level of members of an ecosystem. As part of a stable isotope biogeochemistry and forensics course at Purdue University, students are introduced to this concept by analyzing nitrogen isotopes in sea food purchased from local grocery stores. There is a systematic increase in 15N/14N ratios going from kelp to clams/shrimp, to sardines, to tuna and finally to shark. These enrichments demonstrate how nitrogen is enriched in biomass as predators consume prey. Some of the highest nitrogen isotope enrichments observed, however, are in the common guppy. We investigated a number of aquarium fish foods and find they typically have high nitrogen isotope ratios because they are made form fish meal that is produced primarily from the remains of predator fish such as tuna. From, a isotope perspective, the guppy is the top of the food chain, more ferocious than even the Great White shark.

  6. Detection of Histamine in Fish and Fishery Products in Osaka Prefecture (Fiscal 2015 Report).

    Science.gov (United States)

    Awazu, Kaoru; Takatori, Satoshi; Kakimoto, Sachiko; Nomura, Chie; Masayama, Atsushi; Yamaguchi, Mizuka; Kakimoto, You; Kajimura, Keiji

    2017-01-01

    Histamine food poisoning is caused by ingestion of spoiled fish containing high levels of histamine. This paper reports cases in which histamine was detected in Osaka prefecture in fiscal year 2015 in a survey of fish and fishery products on the market and the food poisoning. A suspected case of histamine food poisoning was also evaluated to investigate the cause and minimize further problems. Histamine in food was separated on SPE cartridge columns, and analyzed after derivatization with fluorescamine by means of HPLC-FL. Histamine was detected in some fishery products on the market and in food that had caused poisoning. The samples in which histamine was detected were semi-dried whole round herring (Urumeiwashi-maruboshi), mackerel (Saba) and sardine dumpling (Iwashi-tsumire). These foods were the main causes of histamine food poisoning according to the report of the Ministry of Health, Labour and Welfare, Government of Japan.

  7. Mercury concentrations in canned and fresh fish and its accumulation in a population of Port Moresby residents.

    Science.gov (United States)

    Kyle, J H; Ghani, N

    1983-01-01

    Five varieties of canned fish and hair specimens from 243 subjects in Port Moresby have been analysed for organic and total mercury. The mean concentrations of total mercury in the canned fish were 0.45 mg/kg for tuna, 0.21 mg/kg for salmon, 0.17 mg/kg for mackerel, 0.08 mg/kg for sardines and 0.04 mg/kg for pilchard, based on the weight of the fish. The mean hair concentration was 2.1 mg/kg, the standard deviation 1.8 mg/kg and the range 0.15 to 14.4 mg/kg. The total mercury levels in hair were significantly higher for coastal people than for non-coastals. Although this is probably due to the greater fish intake of the coastal people, no significant correlation was found between fish consumption and hair mercury levels.

  8. On the "hidden" phytoplankton blooms on Australia's southern shelves

    Science.gov (United States)

    Kämpf, Jochen; Kavi, Ankit

    2017-02-01

    Phytoplankton blooms on Australia's southern shelves are revisited using satellite-derived monthly data of chlorophyll a concentrations for the period 2003-2015. It is known that the region hosts a seasonal coastal upwelling system that develops in austral summer (January-March) with chlorophyll a concentrations of >2 mg/m3. While this summer upwelling is spatially limited to a few hot spots, here we show that widespread phytoplankton blooms of moderate ( 1 mg/m3) chlorophyll a concentrations develop during autumn and early winter on most of Australia's extensive southern shelves—from the vast shelves of the Great Australian Bight (GAB) in the west to Bass Strait in the east. This surprising finding disproves the widespread belief that shelf waters of the GAB are generally oligotrophic and may explain the relatively high abundance of both forage fish (sardines) and upper trophic-level predators (e.g., tuna and whales) in the region.

  9. What is causing the phytoplankton increase in San Francisco Bay?

    Science.gov (United States)

    Cloern, J.E.; Jassby, A.D.; Schraga, T.S.; Dallas, K.L.

    2006-01-01

    The largest living component of San Francisco Bay is the phytoplankton, a suspension of microscopic cells that convert sunlight energy into new living biomass through the same process of photosynthesis used by land plants. This primary production is the ultimate source of food for clams, zooplankton, crabs, sardines, halibut, sturgeon, diving ducks, pelicans, and harbor seals. From measurements made in 1980, we estimated that phytoplankton primary production in San Francisco Bay was about 200,000 tons of organic carbon per year (Jassby et al. 1993). This is equivalent to producing the biomass of 5500 adult humpback whales, or the calories to feed 1.8 million people. These numbers may seem large, but primary production in San Francisco Bay is low compared to many other nutrient-enriched estuaries.

  10. Catch composition and management of daytime purse seine fishery on the Southern Mediterranean Sea Coast, Abu Qir Bay, Egypt

    Directory of Open Access Journals (Sweden)

    A. EL-HAWEET

    2012-12-01

    Full Text Available The present work aimed to obtain precise data on the catch of the purse seine net operating at daytime from sunrise till noon in Abu Qir Bay during 1997 and 1998. Sardine, anchovy, mullet and rabbitfish were recorded as the main target fish groups. Twenty-seven species were recorded as by-catch species. Catch per unit effort was estimated to be 93 kg/day/boat. The percentage of each target species in the catch and its length frequency distribution were presented. The length range of by-catch species and the season of catch were recorded. Daytime purse seine in Abu Qir Bay is an effective method but should regulated by directing the fishing to deeper waters outside the bay rather than the inshore coastal area to conserve many economic species of this important fishery.

  11. Catch of pelagic hauls in Mediterranean acoustic surveys: Is it the same between day and night?

    Directory of Open Access Journals (Sweden)

    Athanassios Machias

    2013-03-01

    Full Text Available Fish sampling is a critical aspect of acoustic surveys, because it is directly related to the “transformation” of echo into species biomass and subsequently affects the accuracy of acoustic estimates. In the present study, we investigated the differences between day and night sampling in a the catch composition through certain diversity indices and b the length frequency distribution of anchovy and sardine using catch data of pelagic hauls collected from four different regions of the European Mediterranean waters. In addition, the possible bias in trawl efficiency due to sampling time and the possible error introduced in acoustic estimates were investigated. No statistically significant differences were found between day and night in any of the parameters examined. The results showed that a more flexible strategy can be adopted to reduce the duration and the cost of acoustic sampling for small pelagic species. The advantages and disadvantages of the two sampling strategies are discussed.

  12. Dimethylamine and diet.

    Science.gov (United States)

    Mitchell, S C; Zhang, A Q; Smith, R L

    2008-05-01

    Forty-six different foods eaten by six healthy male volunteers were investigated as potential sources of the aliphatic secondary amine, dimethylamine. None that were representatives from the fruit and vegetable, meat, dairy and grain produce categories afforded any measurable elevation in urinary dimethylamine output following ingestion. All of the statistically significant increases occurred after consumption of fish and seafoods. However, within this category a wide variation was observed. The highest values were obtained for coley, squid and whiting with cod, haddock, sardine, skate and swordfish also producing substantial increases. Freshwater trout, plaice and prawns gave no discernable effect. It seems that not all fish and seafoods may be treated equally with regards to human dimethylamine exposure and that the situation is more complicated than at first appears.

  13. Utilization of fish meal and fish oil for production of Cryptococcus sp. MTCC 5455 lipase and hydrolysis of polyurethane thereof

    OpenAIRE

    2015-01-01

    Fish meal has been used as an additional nitrogen source and fish oil as inducer for the growth and production of lipase from Cryptococcus sp. MTCC 5455. A response surface design illustrated that the optimum factors influencing lipase production were fish meal, 1.5 %, w/v, Na2HPO4, 0.2 %, w/v, yeast extract, 0.25 %, w/v and sardine oil, 2.0 %, w/v with an activity of 71.23 U/mL at 96 h and 25 °C, which was 48.39 % higher than the conventional one-factor-at-a-time method. The crude concentrat...

  14. Mercury concentrations in canned and fresh fish and its accumulation in a population of Port Moresby residents

    Energy Technology Data Exchange (ETDEWEB)

    Kyle, J.H.; Ghani, N.

    1983-01-01

    Five varieties of canned fish and hair specimens from 243 subjects in Port Moresby have been analysed for organic and total mercury. The mean concentrations of total mercury in the canned fish were 0.45 mg/kg for tuna, 0.21 mg/kg for salmon, 0.17 mg/kg for mackerel, 0.08 mg/kg for sardines and 0.04 mg/kg for pilchard, based on the weight of the fish. The mean hair concentration was 2.1 mg/kg, the standard deviation 1.8 mg/kg and the range 0.15 to 14.4 mg/kg. The total mercury levels in hair were significantly higher for coastal people than for non-coastals. Although this is probably due to the greater fish intake of the coastal people, no significant correlation was found between fish consumption and hair mercury levels.

  15. Mercury in canned fish from local markets in the Czech Republic.

    Science.gov (United States)

    Kral, Tomas; Blahova, Jana; Sedlackova, Lenka; Kalina, Jiri; Svobodova, Zdenka

    2017-02-06

    The aim of this survey was to compare total mercury (THg) in canned fish in terms of species and fishing area. A number of 110 samples of canned fish, which were divided by fish species and fishing area, were analysed. The highest THg content in muscle tissue was found in the escolar. In other fish species, the highest level was detected in tuna. Mercury content in tuna differed significantly from mackerel, sardine, and sprat. Mercury content in herring differed significantly from sprat. The order of the fish according to their THg content corresponds to their status in the aquatic food chain. A significant difference was also found between fish caught from the closed Mediterranean and Black Sea and fish caught in the open oceans. In terms of THg content, the results of this study indicate good quality of various species of canned fish caught in worldwide locations.

  16. Ocean fronts drive marine fishery production and biogeochemical cycling

    Science.gov (United States)

    Woodson, C. Brock; Litvin, Steven Y.

    2015-01-01

    Long-term changes in nutrient supply and primary production reportedly foreshadow substantial declines in global marine fishery production. These declines combined with current overfishing, habitat degradation, and pollution paint a grim picture for the future of marine fisheries and ecosystems. However, current models forecasting such declines do not account for the effects of ocean fronts as biogeochemical hotspots. Here we apply a fundamental technique from fluid dynamics to an ecosystem model to show how fronts increase total ecosystem biomass, explain fishery production, cause regime shifts, and contribute significantly to global biogeochemical budgets by channeling nutrients through alternate trophic pathways. We then illustrate how ocean fronts affect fishery abundance and yield, using long-term records of anchovy–sardine regimes and salmon abundances in the California Current. These results elucidate the fundamental importance of biophysical coupling as a driver of bottom–up vs. top–down regulation and high productivity in marine ecosystems. PMID:25624488

  17. Extraction de lipides en voie aqueuse par bioréacteur enzymatique combine à l'ultrafiltration : application a la valorisation de co-produits de poisson (Sardina pilchardus)

    OpenAIRE

    Dumay, Justine

    2006-01-01

    Ce travail s'inscrit dans le cadre de la valorisation des co-produits issus de la chaîne de transformation de sardine (Sardina pilchardus) en surimi par la mise en oeuvre de techniques douces visant à un investissement et une dépense énergétique modérés afin d'obtenir des composés d'intérêt. Les techniques d'hydrolyse enzymatique et de séparation membranaire ont été choisies pour cette étude. Les enzymes retenues ont tout d'abord été étalonnées de façon à déterminer leurs conditions opéra...

  18. A fuzzy-logic tool for multi-criteria decision making in fisheries: the case of the South African pelagic fishery

    DEFF Research Database (Denmark)

    Paterson, B.; Jarre, Astrid; Moloney, C.L.

    2007-01-01

    The present study presents an electronic decision-support tool that uses a fuzzy-logic model of expert knowledge to assist in multi-criteria decision-making in the context of an Ecosystem Approach to Fisheries (EAF). The prototype model integrates the multiple goals and objectives related...... to the evaluation of the ecosystem performance of the South African sardine Sardinops sagax fishery into a NetWeaver knowledge base and provides intuitive visual outputs to communicate results to managers and stakeholders. The software tool was developed in a consultative process with key experts and follows...... on expert opinion. Results show that the model is robust and conservative. The strength of the approach lies in the ability to include variables that are difficult to measure. It provides a means of rendering value judgements explicit and transparent. The tool synthesises a large amount of information...

  19. Forage fish, their fisheries, and their predators: who drives whom?

    DEFF Research Database (Denmark)

    Engelhard, Georg H.; Peck, Myron A.; Rindorf, Anna;

    2014-01-01

    The North Sea has a diverse forage fish assemblage, including herring, targeted for human consumption; sandeel, sprat, and Norway pout, exploited by industrial fisheries; and some sardine and anchovy, supporting small-scale fisheries. All show large abundance fluctuations, impacting on fisheries...... exist, as in the North Sea. Sandeel appears to be the most important prey forage fish. Seabirds are most dependent on forage fish, due to specialized diet and distributional constraints (breeding colonies). Other than fisheries, key predators of forage fish are a few piscivorous fish species including...... saithe, whiting, mackerel, and horse-mackerel, exploited in turn by fisheries; seabirds and seals have a more modest impact. Size-based foodwebmodelling suggests that reducing fishing mortality may not necessarily lead to larger stocks of piscivorous fish, especially if their early life stages compete...

  20. Food limitation of sea lion pups and the decline of forage off central and southern California.

    Science.gov (United States)

    McClatchie, Sam; Field, John; Thompson, Andrew R; Gerrodette, Tim; Lowry, Mark; Fiedler, Paul C; Watson, William; Nieto, Karen M; Vetter, Russell D

    2016-03-01

    California sea lions increased from approximately 50 000 to 340 000 animals in the last 40 years, and their pups are starving and stranding on beaches in southern California, raising questions about the adequacy of their food supply. We investigated whether the declining sea lion pup weight at San Miguel rookery was associated with changes in abundance and quality of sardine, anchovy, rockfish and market squid forage. In the last decade off central California, where breeding female sea lions from San Miguel rookery feed, sardine and anchovy greatly decreased in biomass, whereas market squid and rockfish abundance increased. Pup weights fell as forage food quality declined associated with changes in the relative abundances of forage species. A model explained 67% of the variance in pup weights using forage from central and southern California and 81% of the variance in pup weights using forage from the female sea lion foraging range. A shift from high to poor quality forage for breeding females results in food limitation of the pups, ultimately flooding animal rescue centres with starving sea lion pups. Our study is unusual in using a long-term, fishery-independent dataset to directly address an important consequence of forage decline on the productivity of a large marine predator. Whether forage declines are environmentally driven, are due to a combination of environmental drivers and fishing removals, or are due to density-dependent interactions between forage and sea lions is uncertain. However, declining forage abundance and quality was coherent over a large area (32.5-38° N) for a decade, suggesting that trends in forage are environmentally driven.