WorldWideScience

Sample records for sanicro 70

  1. Thermomechanical fatigue and damage mechanisms in Sanicro 25 steel

    Czech Academy of Sciences Publication Activity Database

    Petráš, Roman; Škorík, Viktor; Polák, Jaroslav

    2016-01-01

    Roč. 650, JAN (2016), s. 52-62 ISSN 0921-5093 R&D Projects: GA MŠk(CZ) EE2.3.30.0063; GA ČR(CZ) GA13-23652S Institutional support: RVO:68081723 Keywords : thermomechanical fatigue * Sanicro 25 steel * damage mechanism * FIB cutting * localized oxidation-cracking Subject RIV: JL - Materials Fatigue, Friction Mechanics Impact factor: 3.094, year: 2016

  2. Tensile and Creep Testing of Sanicro 25 Using Miniature Specimens

    Science.gov (United States)

    Dymáček, Petr; Jarý, Milan; Dobeš, Ferdinand; Kloc, Luboš

    2018-01-01

    Tensile and creep properties of new austenitic steel Sanicro 25 at room temperature and operating temperature 700 °C were investigated by testing on miniature specimens. The results were correlated with testing on conventional specimens. Very good agreement of results was obtained, namely in yield and ultimate strength, as well as short-term creep properties. Although the creep rupture time was found to be systematically shorter and creep ductility lower in the miniature test, the minimum creep rates were comparable. The analysis of the fracture surfaces revealed similar ductile fracture morphology for both specimen geometries. One exception was found in a small area near the miniature specimen edge that was cut by electro discharge machining, where an influence of the steel fracture behavior at elevated temperature was identified. PMID:29337867

  3. Tensile and Creep Testing of Sanicro 25 Using Miniature Specimens.

    Science.gov (United States)

    Dymáček, Petr; Jarý, Milan; Dobeš, Ferdinand; Kloc, Luboš

    2018-01-16

    Tensile and creep properties of new austenitic steel Sanicro 25 at room temperature and operating temperature 700 °C were investigated by testing on miniature specimens. The results were correlated with testing on conventional specimens. Very good agreement of results was obtained, namely in yield and ultimate strength, as well as short-term creep properties. Although the creep rupture time was found to be systematically shorter and creep ductility lower in the miniature test, the minimum creep rates were comparable. The analysis of the fracture surfaces revealed similar ductile fracture morphology for both specimen geometries. One exception was found in a small area near the miniature specimen edge that was cut by electro discharge machining, where an influence of the steel fracture behavior at elevated temperature was identified.

  4. Low cycle fatigue behavior of Sanicro25 steel at room and at elevated temperature

    Czech Academy of Sciences Publication Activity Database

    Polák, Jaroslav; Petráš, Roman; Heczko, Milan; Kuběna, Ivo; Kruml, Tomáš; Chai, G.

    2014-01-01

    Roč. 615, OKT (2014), s. 175-182 ISSN 0921-5093 R&D Projects: GA ČR(CZ) GA13-23652S Institutional support: RVO:68081723 Keywords : Sanicro 25 steel * Cyclic plasticity * Dislocation structure * Fatigue life * Effect of temperature Subject RIV: JL - Materials Fatigue, Friction Mechanics Impact factor: 2.567, year: 2014

  5. Surface Relief and Internal Structure in Fatigued Stainless Sanicro 25 Steel

    Czech Academy of Sciences Publication Activity Database

    Polák, Jaroslav; Kuběna, Ivo; Mazánová, Veronika; Heczko, Milan; Man, Jiří

    47A, č. 5 (2016), s. 1907-1911 ISSN 1073-5623 R&D Projects: GA ČR GA13-32665S; GA ČR(CZ) GA13-23652S; GA MŠk(CZ) ED1.1.00/02.0068 Institutional support: RVO:68081723 Keywords : surface relief * dislocation structure * Sanicro 25 * extrusion * intrusion Subject RIV: JL - Materials Fatigue, Friction Mechanics Impact factor: 1.874, year: 2016

  6. Surface profile evolution and fatigue crack initiation in Sanicro 25 steel at room temperature

    Czech Academy of Sciences Publication Activity Database

    Polák, Jaroslav; Petráš, Roman; Chai, G.; Škorík, Viktor

    2016-01-01

    Roč. 658, MAR (2016), s. 221-228 ISSN 0921-5093 R&D Projects: GA MŠk(CZ) ED1.1.00/02.0068; GA MŠk(CZ) EE2.3.30.0063; GA ČR(CZ) GA13-23652S Institutional support: RVO:68081723 Keywords : Sanicro 25 steel * Fatigue crack initiation * Persistent slip markings * Extrusions * Intrusions Subject RIV: JL - Materials Fatigue, Friction Mechanics Impact factor: 3.094, year: 2016

  7. Microstructure and dislocation arrangements in Sanicro 25 steel fatigued at ambient and elevated temperatures

    Czech Academy of Sciences Publication Activity Database

    Heczko, Milan; Polák, Jaroslav; Kruml, Tomáš

    2017-01-01

    Roč. 680, JAN (2017), s. 168-181 ISSN 0921-5093 R&D Projects: GA MŠk LM2015069; GA MŠk(CZ) LQ1601; GA ČR(CZ) GA13-23652S Institutional support: RVO:68081723 Keywords : Sanicro 25 * Z-phase * Low cycle fatigue * Low cycle fatigue * Transmission Subject RIV: JL - Materials Fatigue, Friction Mechanics OBOR OECD: Audio engineering, reliability analysis Impact factor: 3.094, year: 2016

  8. Low cycle fatigue behavior of Sanicro25 steel at room and at elevated temperature

    Energy Technology Data Exchange (ETDEWEB)

    Polák, Jaroslav, E-mail: polak@ipm.cz [Institute of Physics of Materials, Academy of Sciences of the Czech Republic, Žižkova 22, 616 62 Brno (Czech Republic); CEITEC, Institute of Physics of Materials Academy of Sciences of the Czech Republic, Žižkova 22, Brno (Czech Republic); Petráš, Roman; Heczko, Milan; Kuběna, Ivo [Institute of Physics of Materials, Academy of Sciences of the Czech Republic, Žižkova 22, 616 62 Brno (Czech Republic); Kruml, Tomáš [Institute of Physics of Materials, Academy of Sciences of the Czech Republic, Žižkova 22, 616 62 Brno (Czech Republic); CEITEC, Institute of Physics of Materials Academy of Sciences of the Czech Republic, Žižkova 22, Brno (Czech Republic); Chai, Guocai [Sandvik Materials Technology, SE-811 81 Sandviken (Sweden); Linköping University, Engineering Materials, SE-581 83 Linköping (Sweden)

    2014-10-06

    Austenitic heat resistant Sanicro 25 steel developed for high temperature applications in power generation industry has been subjected to strain controlled low cycle fatigue tests at ambient and at elevated temperature in a wide interval of strain amplitudes. Fatigue hardening/softening curves, cyclic stress–strain curves and fatigue life curves were evaluated at room temperature and at 700 °C. The internal dislocation structures of the material at room and at elevated temperature were studied using transmission electron microscopy. High resolution surface observations and FIB cuts revealed early damage at room temperature in the form of persistent slip bands and at elevated temperature as oxidized grain boundary cracks. Dislocation arrangement study and surface observations were used to identify the cyclic slip localization and to discuss the fatigue softening/hardening behavior and the temperature dependence of the fatigue life.

  9. Evaluation of Creep Strength of Heterogeneous Welded Joint in HR6W Alloy and Sanicro 25 Steel

    Directory of Open Access Journals (Sweden)

    Zieliński A.

    2017-12-01

    Full Text Available This article presents the results of investigations on HR6W alloy and Sanicro 25 steel and the dissimilar welded joint made of them. The characteristic images of microstructure of the investigated materials in the as-received condition and following the creep test, observed with a scanning electron microscope (SEM, are shown. The X-ray analysis of phase composition of the existing precipitates was carried out. The method for evaluation of creep strength based on abridged creep tests carried out at a temperature higher than the design one is presented. The obtained results do not deviate from the values of creep strength determined in long-term creep tests. The maximum difference in creep strength of the investigated materials is ±20%, which is in compliance with the acceptable scatter band. The methodology presented can be used for verification of creep strength (life time of the material of finished components to be operated under creep conditions.

  10. Jaekaubandusettevõtete TOP 70

    Index Scriptorium Estoniae

    2005-01-01

    Jaekaubandusettevõtete TOP 70; Käibe TOP 25; Kasumi TOP 25; Käibe kasvu TOP 20; Kasumi kasvu TOP 20; Rentaabluse TOP 20; Omakapitali tootlikkuse TOP 20; Jaekaubandusettevõtete üld- ja finantsandmed

  11. MCTS (Exams 70-640, 70-642 and 70-643)

    CERN Document Server

    Panek, William

    2011-01-01

    The must-have study guide for all three Windows Server 2008 R2 MCTS examsNetwork administrators boost their value to their employers with certification, and Microsoft?s three Windows Server 2008 exams offer certification specialties in configuring Active Directory, Network Infrastructure, and Applications Infrastructure. With complete coverage to prepare you for all three exams, this comprehensive study guide has three times the value. Real-world scenarios and hands-on exercises supplement the information to facilitate learning.The three Windows Server 2008 R2 exams (70-640, 70-642, and 70-643

  12. Ehitusmaterjalitootjate TOP 70 aastal 2003

    Index Scriptorium Estoniae

    2004-01-01

    Ilmunud ka: Delovõje Vedomosti : Stroitelstvo, 29. sept. 2004, lk. 2,4. Ehitusmaterjalitootjate TOP 70; Käibe TOP 10; Käibe kasvu TOP 10; Kasumi TOP 10; Kasumi kasvu TOP 10; Rentaabluse TOP 10; Omakapitali tootlikkuse TOP. Ehitusmaterjalitootjate üldandmed

  13. 70 years of woody plants

    DEFF Research Database (Denmark)

    Leverenz, Jerry

    2006-01-01

    On 16 May the Arboretum in Hørsholm celebrates its 70th anniversary. There will be offering guided tours in the evening at 6.30 p.m.. Furthermore, the Arboretum is using the anniversary year to increase public knowledge of the Arboretum and of KVL....

  14. Detection of irradiation-induced, membrane heat shock protein 70 (Hsp70) in mouse tumors using Hsp70 Fab fragment

    International Nuclear Information System (INIS)

    Stangl, Stefan; Themelis, George; Friedrich, Lars; Ntziachristos, Vasilis; Sarantopoulos, Athanasios; Molls, Michael; Skerra, Arne; Multhoff, Gabriele

    2011-01-01

    Background and purpose: The major stress-inducible heat shock protein 70 (Hsp70) is frequently overexpressed in highly aggressive tumors, and elevated intracellular Hsp70 levels mediate protection against apoptosis. Following therapeutic intervention, such as ionizing irradiation, translocation of cytosolic Hsp70 to the plasma membrane is selectively increased in tumor cells and therefore, membrane Hsp70 might serve as a therapy-inducible, tumor-specific target structure. Materials and methods: Based on the IgG1 mouse monoclonal antibody (mAb) cmHsp70.1, we produced the Hsp70-specific recombinant Fab fragment (Hsp70 Fab), as an imaging tool for the detection of membrane Hsp70 positive tumor cells in vitro and in vivo. Results: The binding characteristics of Hsp70 Fab towards mouse colon (CT26) and pancreatic (1048) carcinoma cells at 4 deg. C were comparable to that of cmHsp70.1 mAb, as determined by flow cytometry. Following a temperature shift to 37 deg. C, Hsp70 Fab rapidly translocates into subcellular vesicles of mouse tumor cells. Furthermore, in tumor-bearing mice Cy5.5-conjugated Hsp70 Fab, but not unrelated IN-1 control Fab fragment (IN-1 ctrl Fab), gradually accumulates in CT26 tumors between 12 and 55 h after i.v. injection. Conclusions: In summary, the Hsp70 Fab provides an innovative, low immunogenic tool for imaging of membrane Hsp70 positive tumors, in vivo.

  15. Nuclear Data Sheets for A = 70

    Energy Technology Data Exchange (ETDEWEB)

    Gürdal, G.; McCutchan, E.A.

    2016-09-15

    Spectroscopic data for all nuclei with mass number A = 70 have been evaluated, and the corresponding level schemes from radioactive decay and reaction studies are presented. Since the previous evaluation, the half-life of {sup 70}Mn has been measured and excited states in {sup 70}Fe observed for the first time. Excited states in {sup 70}Ni have been more extensively studied while Coulomb excitation and collinear laser spectroscopy measurements in {sup 70}Cu have allowed for firm Jπ assignments. Despite new measurements, there remain some discrepancies in half-lives of low lying states in {sup 70}Zn. New measurements have extended the knowledge of high-spin band structures in {sup 70}Ge and {sup 70}As. This evaluation supersedes the prior A = 70 evaluation of 2004Tu09.

  16. STS-70 Post Flight Presentation

    Science.gov (United States)

    Peterson, Glen (Editor)

    1995-01-01

    In this post-flight overview, the flight crew of the STS-70 mission, Tom Henricks (Cmdr.), Kevin Kregel (Pilot), Major Nancy Currie (MS), Dr. Mary Ellen Weber (MS), and Dr. Don Thomas (MS), discuss their mission and accompanying experiments. Pre-flight, launch, and orbital footage is followed by the in-orbit deployment of the Tracking and Data Relay Satellite (TDRS) and a discussion of the following spaceborne experiments: a microgravity bioreactor experiment to grow 3D body-like tissue; pregnant rat muscular changes in microgravity; embryonic development in microgravity; Shuttle Amateur Radio Experiment (SAREX); terrain surface imagery using the HERCULES camera; and a range of other physiological tests, including an eye and vision test. Views of Earth include: tropical storm Chantal; the Nile River and Red Sea; lightning over Brazil. A three planet view (Earth, Mars, and Venus) was taken right before sunrise. The end footage shows shuttle pre-landing checkout, entry, and landing, along with a slide presentation of the flight.

  17. Vadim Loktev's 70th birthday

    Directory of Open Access Journals (Sweden)

    Editorial Board

    2015-06-01

    Full Text Available This year Vadim Loktev, a well-known scientist, prominent theoretical physicist, talented lecturer, organizer of scientific research and activity celebrates his 70th birthday. Vadim Loktev was born on May, 3, 1945 in Kyiv (Ukraine; his parents were professional employees. He studied at the Department of Physics of Taras Shevchenko State University (Kyiv. After graduation from the university in 1968 he was a postgraduate student at the Institute for Theoretical Physics of the Academy of Sciences of the Ukrainian SSR [now Bogolyubov Institute for Theoretical Physics of the National Academy of Sciences of Ukraine (NASU] in 1968-1971. He has been working at this institute since 1967 up to the present time now occupying the position of Head of the Department of Nonlinear Condensed Matter Physics. In 1971 V. Loktev defended his Ph.D. thesis under the supervision of the world-renowned scientist A. Davydov; in 1983 he defended his D.Sc. thesis. In 1997 he obtained title of Professor. The same year V. Loktev was elected a corresponding member and in 2003 he was elected a full member of the NASU.

  18. 42 CFR 457.70 - Program options.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Program options. 457.70 Section 457.70 Public... Plans for Child Health Insurance Programs and Outreach Strategies § 457.70 Program options. (a) Health benefits coverage options. A State may elect to obtain health benefits coverage under its plan through— (1...

  19. 7 CFR 70.3 - Administration.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Administration. 70.3 Section 70.3 Agriculture... PRODUCTS AND RABBIT PRODUCTS Grading of Poultry Products and Rabbit Products General § 70.3 Administration... Secretary may require in the enforcement or administration of the provisions of the Act and the regulations...

  20. 21 CFR 201.70 - Calcium labeling.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false Calcium labeling. 201.70 Section 201.70 Food and... LABELING Labeling Requirements for Over-the-Counter Drugs § 201.70 Calcium labeling. (a) The labeling of over-the-counter (OTC) drug products intended for oral ingestion shall contain the calcium content per...

  1. 22 CFR 226.70 - Purpose.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Purpose. 226.70 Section 226.70 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADMINISTRATION OF ASSISTANCE AWARDS TO U.S. NON-GOVERNMENTAL ORGANIZATIONS After-the-Award Requirements § 226.70 Purpose. Sections 226.71 through 226.73 contain closeout...

  2. 7 CFR 70.25 - Political activity.

    Science.gov (United States)

    2010-01-01

    ... Graders § 70.25 Political activity. Federal graders may participate in certain political activities, including management and participation in political campaigns in accordance with AMS policy. Graders are... 7 Agriculture 3 2010-01-01 2010-01-01 false Political activity. 70.25 Section 70.25 Agriculture...

  3. 40 CFR 761.70 - Incineration.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Incineration. 761.70 Section 761.70... and Disposal § 761.70 Incineration. This section applies to facilities used to incinerate PCBs... regular intervals of no longer than 15 minutes. (4) The temperatures of the incineration process shall be...

  4. 40 CFR 262.70 - Farmers.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Farmers. 262.70 Section 262.70 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO GENERATORS OF HAZARDOUS WASTE Farmers § 262.70 Farmers. A farmer disposing of waste pesticides...

  5. 7 CFR 947.70 - Effective time.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Effective time. 947.70 Section 947.70 Agriculture... Effective Time and Termination § 947.70 Effective time. (a) The provisions of this subpart shall become effective at such time as the Secretary may declare above his signature attached to this subpart, and shall...

  6. 7 CFR 958.70 - Effective time.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Effective time. 958.70 Section 958.70 Agriculture... COUNTIES IN IDAHO, AND MALHEUR COUNTY, OREGON Order Regulating Handling Effective Time and Termination § 958.70 Effective time. The provisions of this subpart, or any amendment thereto, shall become...

  7. 7 CFR 1212.70 - Reports.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Reports. 1212.70 Section 1212.70 Agriculture..., Consumer Education, and Industry Information Order Reports, Books, and Records § 1212.70 Reports. (a) Each first handler or importer subject to this part must report to the Board, at the time and in the manner...

  8. 28 CFR 70.24 - Program income.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Program income. 70.24 Section 70.24...-PROFIT ORGANIZATIONS Post-Award Requirements Financial and Program Management § 70.24 Program income. (a... income related to projects financed in whole or in part with Department funds. (b) Except as provided in...

  9. 2 CFR 215.70 - Purpose.

    Science.gov (United States)

    2010-01-01

    ... ORGANIZATIONS (OMB CIRCULAR A-110) After-the-Award Requirements § 215.70 Purpose. Sections 215.71 through 215.73... 2 Grants and Agreements 1 2010-01-01 2010-01-01 false Purpose. 215.70 Section 215.70 Grants and Agreements OFFICE OF MANAGEMENT AND BUDGET CIRCULARS AND GUIDANCE Reserved UNIFORM ADMINISTRATIVE...

  10. 38 CFR 70.30 - Payment principles.

    Science.gov (United States)

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Payment principles. 70.30 Section 70.30 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS (CONTINUED) VHA BENEFICIARY TRAVEL UNDER 38 U.S.C. 111 § 70.30 Payment principles. (a) Subject to the other provisions of this...

  11. 40 CFR 7.70 - New construction.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false New construction. 7.70 Section 7.70... of Handicap § 7.70 New construction. (a) General. New facilities shall be designed and constructed to... 18, 1991, design, construction, or alteration of buildings in conformance with sections 3-8 of the...

  12. 40 CFR 73.70 - Auctions.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 16 2010-07-01 2010-07-01 false Auctions. 73.70 Section 73.70... ALLOWANCE SYSTEM Auctions, Direct Sales, and Independent Power Producers Written Guarantee § 73.70 Auctions... allowances pursuant to paragraph (c) of this section and § 73.72(q) in the amounts and at the times provided...

  13. 46 CFR 67.70 - Original owner.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Original owner. 67.70 Section 67.70 Shipping COAST GUARD... Acceptable Title Evidence; Waiver § 67.70 Original owner. The builder's certification described in § 67.99 serves as evidence of the original owner's title to a vessel. ...

  14. 28 CFR 70.52 - Financial reporting.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Financial reporting. 70.52 Section 70.52...-PROFIT ORGANIZATIONS Post-Award Requirements Reports and Records § 70.52 Financial reporting. (a) The following forms or such other forms as may be approved by OMB are authorized for obtaining financial...

  15. 27 CFR 4.70 - Application.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Application. 4.70 Section 4.70 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS LABELING AND ADVERTISING OF WINE Standards of Fill for Wine § 4.70 Application...

  16. A preface to the 70 and 70 Gravity Fest

    International Nuclear Information System (INIS)

    Gutiérrez-Piñeres, A. C.; Montoya, E. A.; Núñez, L.A.

    2017-01-01

    There are transcendental landmarks in life to account and balance achievements and failures. With no doubt the age of 70 brings with it the possibility to trace back a significant part of our existences and can considered a natural stop. The year 2016 is, for many Latin American physicists, an important date because Rodolfo Gambini Italiano from the Universidad de la Repblica, Uruguay and Luis (Gaucho) Herrera Cometta from Universidad Central de Venezuela reach this age enjoying the passion for building generations of physicis in Latin America and leaving a profound and important imprint in Theoretical Physics along several countries of our continent. This is a tribute to these two masters, a great opportunity to share their visions and particular stiles with new generations of physics students from Latin America. These two masters of the Theoretical Physics have life-lines that crosses at different instants of their lives. Rodolfo (born May 11, 1946), got degree in physics from the University of the Republic in 1972–In fact, he was the first physicist graduated in Uruguay–. Then, he moved to Paris and obtained his doctorate in Theoretical Physics under the supervision of Achilles Papapetrou. This was the first encounter with El Gaucho. Luis (born December 20, 1946) after graduated from the People’s Friendship University, Moscow went to Paris to study also under the guidance of Papapetrou. Luis returned to Caracas in 1972, taking a position at the Universidad Central de Venezuela and joint Professor Carlos Aragone and Gustavo González Martín, starting a seminal group the Caracas Relativity Seminar. Prof. Aragone, former supervisor of Rodolfo, had just arrived to Caracas after militar coup d’état in Uruguay and got a position at Universidad Simón Bolívar, USB. In 1975 Rodolfo also started to work at USB, invited by Prof Aragone and this is the second cross road between him and El Gaucho. In 1978 Rodolfo and Antoni Trias began a great friendship and a

  17. A preface to the 70&70 Gravity Fest

    Science.gov (United States)

    Gutiérrez-Piñeres, A. C.; Montoya, E. A.; Núñez, L. A.

    2017-03-01

    There are transcendental landmarks in life to account and balance achievements and failures. With no doubt the age of 70 brings with it the possibility to trace back a significant part of our existences and can considered a natural stop. The year 2016 is, for many Latin American physicists, an important date because Rodolfo Gambini Italiano from the Universidad de la Repblica, Uruguay and Luis (Gaucho) Herrera Cometta from Universidad Central de Venezuela reach this age enjoying the passion for building generations of physicis in Latin America and leaving a profound and important imprint in Theoretical Physics along several countries of our continent. This is a tribute to these two masters, a great opportunity to share their visions and particular stiles with new generations of physics students from Latin America. These two masters of the Theoretical Physics have life-lines that crosses at different instants of their lives. Rodolfo (born May 11, 1946), got degree in physics from the University of the Republic in 1972-In fact, he was the first physicist graduated in Uruguay-. Then, he moved to Paris and obtained his doctorate in Theoretical Physics under the supervision of Achilles Papapetrou. This was the first encounter with El Gaucho. Luis (born December 20, 1946) after graduated from the People’s Friendship University, Moscow went to Paris to study also under the guidance of Papapetrou. Luis returned to Caracas in 1972, taking a position at the Universidad Central de Venezuela and joint Professor Carlos Aragone and Gustavo González Martín, starting a seminal group the Caracas Relativity Seminar. Prof. Aragone, former supervisor of Rodolfo, had just arrived to Caracas after militar coup d’état in Uruguay and got a position at Universidad Simón Bolívar, USB. In 1975 Rodolfo also started to work at USB, invited by Prof Aragone and this is the second cross road between him and El Gaucho. In 1978 Rodolfo and Antoni Trias began a great friendship and a

  18. 10 CFR 70.7 - Employee protection.

    Science.gov (United States)

    2010-01-01

    ... occurs because the employee has engaged in protected activities. An employee's engagement in protected... 10 Energy 2 2010-01-01 2010-01-01 false Employee protection. 70.7 Section 70.7 Energy NUCLEAR... Employee protection. (a) Discrimination by a Commission licensee, an applicant for a Commission license, or...

  19. 24 CFR 70.3 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Definitions. 70.3 Section 70.3 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development USE OF... of individual volunteers in group insurance plans (such as liability, health, life, disability...

  20. 41 CFR 105-70.021 - Discovery.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Discovery. 105-70.021 Section 105-70.021 Public Contracts and Property Management Federal Property Management Regulations System..., papers, and other data and documentary evidence. Nothing contained herein shall be interpreted to require...

  1. 48 CFR 2052.204-70 - Security.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Security. 2052.204-70... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 2052.204-70 Security. As prescribed... National Security information, restricted data, formerly restricted data, and other classified data...

  2. 29 CFR 102.70 - Runoff election.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 2 2010-07-01 2010-07-01 false Runoff election. 102.70 Section 102.70 Labor Regulations... Runoff election. (a) The regional director shall conduct a runoff election, without further order of the... objections are filed as provided in § 102.69. Only one runoff shall be held pursuant to this section. (b...

  3. 27 CFR 70.244 - Payroll period.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Payroll period. 70.244... Excise and Special (Occupational) Tax Limitations § 70.244 Payroll period. For purpose of determining the... established calendar period regularly used by the employer or other person levied upon for payroll or payment...

  4. 41 CFR 105-70.033 - Witnesses.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Witnesses. 105-70.033 Section 105-70.033 Public Contracts and Property Management Federal Property Management Regulations System... may proceed by leading questions only if the witness is a hostile witness, an adverse party, or a...

  5. 27 CFR 70.93 - Interest rate.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Interest rate. 70.93... Excise and Special (Occupational) Tax Interest § 70.93 Interest rate. (a) In general. The interest rate... annual percentage rate of interest will exceed the prescribed rate of interest. (b) Applicability of...

  6. 7 CFR 982.70 - Confidential information.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Confidential information. 982.70 Section 982.70 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... trade secret or disclosing of the trade position, financial condition, or business operations of the...

  7. 48 CFR 652.100-70 - Policy.

    Science.gov (United States)

    2010-10-01

    ... included. (b) The contracting officer shall justify the exclusion of any provisions or clauses in... 652.100-70 Federal Acquisition Regulations System DEPARTMENT OF STATE CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Instructions for Using Provisions and Clauses 652.100-70 Policy. (a) The...

  8. 48 CFR 653.101-70 - Policy.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Policy. 653.101-70 Section 653.101-70 Federal Acquisition Regulations System DEPARTMENT OF STATE CLAUSES AND FORMS FORMS General... exclusion of any form in accordance with FAR subpart 1.4 and 601.470. [53 FR 26180, July 11, 1988, as...

  9. 15 CFR 700.70 - General provisions.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false General provisions. 700.70 Section 700.70 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE NATIONAL SECURITY INDUSTRIAL BASE REGULATIONS...

  10. 46 CFR 111.70-1 - General.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false General. 111.70-1 Section 111.70-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL REQUIREMENTS..., except when a wye-delta or a delta-wye transformer is used. (c) The motor disconnecting means must be an...

  11. 17 CFR 200.70 - Business promotions.

    Science.gov (United States)

    2010-04-01

    ... AND ETHICS; AND INFORMATION AND REQUESTS Canons of Ethics § 200.70 Business promotions. A member must not engage in any other business, employment or vocation while in office, nor may he ever use the... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Business promotions. 200.70...

  12. 46 CFR 147.70 - Acetylene.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Acetylene. 147.70 Section 147.70 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) DANGEROUS CARGOES HAZARDOUS SHIPS' STORES Stowage and Other... standard cubic feet) of acetylene may be on board a vessel engaged in industrial operations, if it is...

  13. 27 CFR 70.609 - Supervision.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Supervision. 70.609 Section 70.609 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY (CONTINUED) PROCEDURES AND PRACTICES PROCEDURE AND ADMINISTRATION Losses Resulting From Disaster, Vandalism, or Malicious Mischief...

  14. 7 CFR 70.12 - Supervision.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Supervision. 70.12 Section 70.12 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) REGULATIONS AND STANDARDS UNDER THE AGRICULTURAL MARKETING ACT OF 1946 AND THE EGG...

  15. MCSE Core Elective Exams in a Nutshell Covers exams 70-270, 70-297, and 70-298

    CERN Document Server

    Bhardwaj, Pawan

    2006-01-01

    For Windows XP and Windows Server 2003 administrators preparing forMicrosoft Certified Systems Engineer (MCSE) core exam 70-270, and core exams 70-297, and 70-298, this book is invaluable. Not only does it provide the resources you need to succeed on the exams, but to succeed in the real world as well. Think of this book as the notes you would have taken if you were to capture every essential nugget of information related to the skills measured in the core elective exams. To begin with, MCSE Core Elective Exams in a Nutshell allows you to see all of the topics expected for mastery in each

  16. 70 kD stress protein (Hsp70) analysis in living shallow-water benthic foraminifera

    Digital Repository Service at National Institute of Oceanography (India)

    Heinz, P.; Marten, R.A; Linshy, V.N.; Haap, T.; Geslin, E.; Kohler, H-R.

    Hsp70 is a phylogenetically highly conserved protein family present in all eukaryotic organisms tested so far. Its synthesis is induced by proteotoxic stress. The detection of Hsp70 in foraminifera is presented here. We introduce a standard...

  17. 27 CFR 70.485 - Closing agreements.

    Science.gov (United States)

    2010-04-01

    ... Relating to Alcohol, Tobacco, Firearms, and Explosives Administrative Remedies § 70.485 Closing agreements... disadvantage through consummation of such an agreement. (b) Scope of closing agreement—(1) In general. A...

  18. 28 CFR 70.31 - Insurance coverage.

    Science.gov (United States)

    2010-07-01

    ... with Federal funds as provided to property owned by the recipient. Federally-owned property need not be...-PROFIT ORGANIZATIONS Post-Award Requirements Property Standards § 70.31 Insurance coverage. Recipients...

  19. 48 CFR 952.208-70 - Printing.

    Science.gov (United States)

    2010-10-01

    ... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 952.208-70 Printing. As prescribed... reproduction in excess of the limits set forth above, the Contractor shall notify the Contracting Officer in...

  20. 41 CFR 105-70.002 - Definitions.

    Science.gov (United States)

    2010-07-01

    ...-IMPLEMENTATION OF THE PROGRAM FRAUD CIVIL REMEDIES ACT OF 1986 § 105-70.002 Definitions. The following shall have... representation, certification, affirmation, document, record, or accounting or bookkeeping entry made— (1) With...

  1. Solving the shape conundrum in $^{70}$Se

    CERN Multimedia

    We propose a multi-step Coulomb-excitation study of $^{70}$Se at HIE-ISOLDE using the $^{208}$Pb( $^{70}$Se, $^{70}$Se*)$^{208}$Pb* reaction at a safe energy of 5.0 MeV/u. We aim at a precise measurement of the $\\left \\langle 2^{+}_{1} \\hspace{0.1cm} || \\hspace{0.1cm}\\hat{E}2 \\hspace{0.1cm} || \\hspace{0.1cm}2^{+}_{ 1} \\right \\rangle$ diagonal matrix element as well as gaining information on additional matrix elements. Such information will shed light onto the shape conundrum of the 2$^{+}_{1}$ state in $^{70}$Se as well as foreseeing the opportunity for a more detailed understanding of the shape-coexistence phenomenon in this region.

  2. Collectivity in A ∼ 70 nuclei studied via lifetime measurements in {sup 70}Br and {sup 68,70}Se

    Energy Technology Data Exchange (ETDEWEB)

    Nichols, A.J., E-mail: an527@york.ac.uk [Department of Physics, University of York, Heslington, York YO10 5DD (United Kingdom); Wadsworth, R. [Department of Physics, University of York, Heslington, York YO10 5DD (United Kingdom); Iwasaki, H. [National Superconducting Cyclotron Laboratory and Department of Physics and Astronomy, Michigan State University, 164 S. Shaw Lane, East Lansing, MI 48825-1321 (United States); Kaneko, K. [Department of Physics, Kyushu Sangyo University, Fukuoka 813-8503 (Japan); Lemasson, A. [National Superconducting Cyclotron Laboratory and Department of Physics and Astronomy, Michigan State University, 164 S. Shaw Lane, East Lansing, MI 48825-1321 (United States); Grand Accélérateur National d' Ions Lourds, Bd. Henri Becquerel, BP 55027, F-14076 Caen Cedex 05 (France); Angelis, G. de [Laboratori Nazionali di Legnaro dell' INFN, Legnaro (Padova) I-35020 (Italy); Bader, V.M.; Baugher, T.; Bazin, D. [National Superconducting Cyclotron Laboratory and Department of Physics and Astronomy, Michigan State University, 164 S. Shaw Lane, East Lansing, MI 48825-1321 (United States); Bentley, M.A. [Department of Physics, University of York, Heslington, York YO10 5DD (United Kingdom); Berryman, J.S. [National Superconducting Cyclotron Laboratory and Department of Physics and Astronomy, Michigan State University, 164 S. Shaw Lane, East Lansing, MI 48825-1321 (United States); Braunroth, T. [Institut für Kernphysik der Universität zu Köln, Köln 50937 (Germany); Davies, P.J. [Department of Physics, University of York, Heslington, York YO10 5DD (United Kingdom); and others

    2014-06-02

    Transition strengths for decays from low-lying states in A ∼ 70 nuclei have been deduced from lifetime measurements using the recoil distance Doppler shift technique. The results confirm the collectivity previously reported for the 2{sub 1}{sup +}→0{sub gs}{sup +} decay in {sup 68}Se and reveal a relative decrease in collectivity in {sup 70}Br. This trend is reproduced by shell model calculations using the GXPF1A interaction in an fp model space including the Coulomb, spin-orbit and isospin non-conserving interactions. The 3{sub 1}{sup +}→2{sub 1}{sup +} decay in {sup 70}Br is found to have a very small B(M1) value, which is consistent with the configuration of the state being dominated by the coupling of f{sub 5/2} protons and neutrons. The results suggest that the g{sub 9/2} orbit does not play an important role at low spin in these nuclei. The B(E2) values for the decays of the (T = 1) 2{sub 1}{sup +} states in {sup 70}Br and {sup 70}Se are almost identical, suggesting there is no major shape change between the two nuclei at low spin.

  3. Collectivity in A ∼ 70 nuclei studied via lifetime measurements in 70 Br and 68,70 Se

    OpenAIRE

    Nichols, A.J.; Wadsworth, R.; Iwasaki, H.; Kaneko, K.; Lemasson, A.; de Angelis, G.; Bader, V.M.; Baugher, T.; Bazin, D.; Bentley, M.A.; Berryman, J.S.; Braunroth, T.; Davies, P.J.; Dewald, A.; Fransen, C.

    2016-01-01

    Transition strengths for decays from low-lying states in A ∼ 70 nuclei have been deduced from lifetime measurements using the recoil distance Doppler shift technique. The results confirm the collectivity previously reported for the 21+→0gs+ decay in 68 Se and reveal a relative decrease in collectivity in 70 Br. This trend is reproduced by shell model calculations using the GXPF1A interaction in an fp model space including the Coulomb, spin-orbit and isospin non-conserving interactions. The 31...

  4. Characterization of the Antibiotic Compound No. 70 Produced by Streptomyces sp. IMV-70

    Science.gov (United States)

    Trenozhnikova, Lyudmila P.; Khasenova, Almagul K.; Balgimbaeva, Assya S.; Fedorova, Galina B.; Katrukha, Genrikh S.; Tokareva, Nina L.; Kwa, Boo H.; Azizan, Azliyati

    2012-01-01

    We describe the actinomycete strain IMV-70 isolated from the soils of Kazakhstan, which produces potent antibiotics with high levels of antibacterial activity. After the research of its morphological, chemotaxonomic, and cultural characteristics, the strain with potential to be developed further as a novel class of antibiotics with chemotherapeutics potential was identified as Streptomyces sp. IMV-70. In the process of fermentation, the strain Streptomyces spp. IMV-70 produces the antibiotic no. 70, which was isolated from the culture broth by extraction with organic solvents. Antibiotic compound no. 70 was purified and separated into individual components by HPLC, TLC, and column chromatography methods. The main component of the compound is the antibiotic 70-A, which was found to be identical to the peptolide etamycin A. Two other antibiotics 70-B and 70-C have never been described and therefore are new antibiotics. The physical-chemical and biological characteristics of these preparations were described and further researched. Determination of the optimal growth conditions to cultivate actinomycete-producer strain IMV-70 and development of methods to isolate, purify, and accumulate preparations of the new antibiotic no. 70 enable us to research further the potential of this new class of antibiotics. PMID:22536145

  5. Characterization of the Antibiotic Compound No. 70 Produced by Streptomyces sp. IMV-70

    Directory of Open Access Journals (Sweden)

    Lyudmila P. Trenozhnikova

    2012-01-01

    Full Text Available We describe the actinomycete strain IMV-70 isolated from the soils of Kazakhstan, which produces potent antibiotics with high levels of antibacterial activity. After the research of its morphological, chemotaxonomic, and cultural characteristics, the strain with potential to be developed further as a novel class of antibiotics with chemotherapeutics potential was identified as Streptomyces sp. IMV-70. In the process of fermentation, the strain Streptomyces spp. IMV-70 produces the antibiotic no. 70, which was isolated from the culture broth by extraction with organic solvents. Antibiotic compound no. 70 was purified and separated into individual components by HPLC, TLC, and column chromatography methods. The main component of the compound is the antibiotic 70-A, which was found to be identical to the peptolide etamycin A. Two other antibiotics 70-B and 70-C have never been described and therefore are new antibiotics. The physical-chemical and biological characteristics of these preparations were described and further researched. Determination of the optimal growth conditions to cultivate actinomycete-producer strain IMV-70 and development of methods to isolate, purify, and accumulate preparations of the new antibiotic no. 70 enable us to research further the potential of this new class of antibiotics.

  6. IFCI 7.0 Models and Correlations

    Energy Technology Data Exchange (ETDEWEB)

    Reed, A.W.; Schmidt, R.C.; Young, M.F.

    1999-05-01

    The Integrated Fuel-Coolant Interaction Code (IFCI) is a best-estimate computer program for analysis of phenomena related to mixing of molten nuclear reactor core material with reactor coolant (water). The stand-alone version of the code, IFCI 7.0, has been designed for analysis of small- and intermediate-scale experiments in order to gain insight into the physics (including scaling effects) of molten fuel-coolant interactions. The code's methods, models, and correlations are being assessed. This report describes the flow regime, friction factor, and heat-transfer models used in the current version of IFCI (IFCI 7.0).

  7. High spin spectroscopy of 70Ge

    International Nuclear Information System (INIS)

    Kumar Raju, M.; Sugathan, P.; Seshi Reddy, T.; Thirumala Rao, B.V.; Madhusudhana Rao, P.V.; Muralithar, S.; Singh, R.P.; Bhowmik, R.K.

    2011-01-01

    Structure of nuclei in mass 70 region is of interest due to presence of a variety of complex phenomenon. In these nuclei rapid change of nuclear shape with proton and neutron numbers, spin and excitation energy. Valance nucleons in f-p-g shell configuration will drive the nuclei towards high deformations. Relatively large values of quadrupole deformation are evident in the even-even nuclei in this region. Present study is aimed to explore the high spin structure of the 70 Ge nucleus. A negative parity structure was reported in an earlier study

  8. IFCI 7.0 Models and Correlations

    International Nuclear Information System (INIS)

    Reed, A.W.; Schmidt, R.C.; Young, M.F.

    1999-01-01

    The Integrated Fuel-Coolant Interaction Code (IFCI) is a best-estimate computer program for analysis of phenomena related to mixing of molten nuclear reactor core material with reactor coolant (water). The stand-alone version of the code, IFCI 7.0, has been designed for analysis of small- and intermediate-scale experiments in order to gain insight into the physics (including scaling effects) of molten fuel-coolant interactions. The code's methods, models, and correlations are being assessed. This report describes the flow regime, friction factor, and heat-transfer models used in the current version of IFCI (IFCI 7.0)

  9. 10 CFR 455.70 - Purpose.

    Science.gov (United States)

    2010-01-01

    ... ENERGY CONSERVATION GRANT PROGRAMS FOR SCHOOLS AND HOSPITALS AND BUILDINGS OWNED BY UNITS OF LOCAL GOVERNMENT AND PUBLIC CARE INSTITUTIONS Energy Conservation Measures for Schools and Hospitals § 455.70... energy conservation measures, including renewable resource measures, and the elements of an energy...

  10. 28 CFR 70.36 - Intangible property.

    Science.gov (United States)

    2010-07-01

    ...-PROFIT ORGANIZATIONS Post-Award Requirements Property Standards § 70.36 Intangible property. (a) The... purchased, under an award. The Department reserves a royalty-free, nonexclusive and irrevocable right to..., peer reviews, or communications with colleagues. This “recorded” material excludes physical objects (e...

  11. 40 CFR 70.6 - Permit content.

    Science.gov (United States)

    2010-07-01

    ... § 70.5(d) of this part. (B) Prompt reporting of deviations from permit requirements, including those... corrective actions or preventive measures taken. The permitting authority shall define “prompt” in relation... and air pollution control equipment), practices, or operations regulated or required under the permit...

  12. 40 CFR 141.70 - General requirements.

    Science.gov (United States)

    2010-07-01

    ...) NATIONAL PRIMARY DRINKING WATER REGULATIONS Filtration and Disinfection § 141.70 General requirements. (a... regulations establish criteria under which filtration is required as a treatment technique for public water... filtration requirements in § 141.73 and the disinfection requirements in § 141.72(b). (c) Each public water...

  13. 27 CFR 70.101 - Bad checks.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Bad checks. 70.101 Section....101 Bad checks. If any check or money order in payment of any amount receivable under Title 26 of the... appropriate TTB officer that such check was tendered in good faith and that such person had reasonable cause...

  14. 10 CFR 70.44 - Creditor regulations.

    Science.gov (United States)

    2010-01-01

    ... Transfer of Special Nuclear Material, Creditors' Rights § 70.44 Creditor regulations. (a) Pursuant to... mortgage, pledge, or other lien upon any special nuclear material, not owned by the United States, which is subject to licensing: Provided: (1) That the rights of any creditor so secured may be exercised only in...

  15. 27 CFR 70.144 - Special rules.

    Science.gov (United States)

    2010-04-01

    ..., under local law, one person is subrogated to the rights of another with respect to a lien or interest, such person shall be subrogated to such rights for purposes of any lien imposed by 26 U.S.C. 6321 or... Excise and Special (Occupational) Tax Lien for Taxes § 70.144 Special rules. (a) Actual notice or...

  16. 27 CFR 70.607 - Supporting evidence.

    Science.gov (United States)

    2010-04-01

    ..., records, labels, formulas, stamps) that is available to submit, relating to the quantities and identities of the liquors, on which duty has been paid or tax has been paid or determined, that were on hand at....607 Section 70.607 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU...

  17. 30 CFR 70.2 - Definitions.

    Science.gov (United States)

    2010-07-01

    .... L. 95-164. (b) Active workings means any place in a coal mine where miners are normally required to... § 70.202 (Certified person; sampling) to take respirable dust samples required by this part or... maintenance and calibration of respirable dust sampling equipment as required by this part. (d) Concentration...

  18. 28 CFR 70.41 - Recipient responsibilities.

    Science.gov (United States)

    2010-07-01

    ... violation of statute are to be referred to such Federal, State or local authority as may have proper... OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Procurement Standards § 70.41 Recipient... responsibilities arising under its contract(s). The recipient is the responsible authority, without recourse to the...

  19. 28 CFR 70.22 - Payment.

    Science.gov (United States)

    2010-07-01

    ... (INCLUDING SUBAWARDS) WITH INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Financial and Program Management § 70.22 Payment. (a) Payment methods must... transfer of funds and disbursement by the recipient, and financial management systems that meet the...

  20. 28 CFR 70.27 - Allowable costs.

    Science.gov (United States)

    2010-07-01

    ... AND AGREEMENTS (INCLUDING SUBAWARDS) WITH INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Financial and Program Management § 70.27 Allowable costs. (a... Organizations.” The allowability of costs incurred by institutions of higher education is determined in...

  1. 15 CFR 922.70 - Boundary.

    Science.gov (United States)

    2010-01-01

    ... a distance of approximately six nmi from the following islands and offshore rocks: San Miguel Island... Rock (the Islands). The seaward boundary coordinates are listed in Appendix A to this subpart. [74 FR... MARINE SANCTUARY PROGRAM REGULATIONS Channel Islands National Marine Sanctuary § 922.70 Boundary. The...

  2. 40 CFR 70.5 - Permit applications.

    Science.gov (United States)

    2010-07-01

    ... establish. Where an existing part 70 permit would prohibit such construction or change in operation, the... information only if it is related to the proposed change. Information required under paragraph (c) of this... part shall state that, based on information and belief formed after reasonable inquiry, the statements...

  3. Mitochondrial membrane assembly of TMEM70 protein

    Czech Academy of Sciences Publication Activity Database

    Kratochvílová, H.; Hejzlarová, Kateřina; Vrbacký, Marek; Mráček, Tomáš; Karbanová, Vendula; Tesařová, M.; Gombitová, A.; Cmarko, D.; Wittig, I.; Zeman, J.; Houštěk, Josef

    2014-01-01

    Roč. 15, Mar 2014 (2014), s. 1-9 ISSN 1567-7249 R&D Projects: GA ČR(CZ) GAP303/11/0970; GA MŠk(CZ) LL1204; GA ČR(CZ) GB14-36804G Institutional support: RVO:67985823 Keywords : mitochondria * ATP synthase * TMEM70 * biogenesis Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.249, year: 2014

  4. US tornadoes. Part 1: 70-year statistics

    Science.gov (United States)

    Fujita, T. T.

    1987-01-01

    If tornadoes were counted as the Gross National Product, no other country on the surface of the earth could come even close to the United States. During the recent 70 year period, the United States produced 31,054 tornadoes which left behind a cumulative path of 132,005 miles (212,396 km) which would circle the world 5.3 times along the equator. In completing the book, staff members of the Satellite and Mesometeorlogy Research Project (1961 to the present) played an important role in collecting, evaluating, and archiving the historical tornado data.

  5. Naisjuhtidega ettevõtete TOP 70

    Index Scriptorium Estoniae

    2003-01-01

    Naisjuhtidega ettevõtete TOP 70. Käibe TOP 30. Käibe kasvu TOP 30. Kasumi TOP 30. Kasumi kasvu TOP 30. Rentaabluse TOP 30. Omakapitali tootlikkuse TOP 30. Nais- ja meesjuhtidega ettevõtted 2001. aasta 500 edukama ettevõtte hulgas. Naistegevjuhtidega firmade TOP 2001 ja 2002. Riigi- ja kohaliku omavalitsuse asutuste naisjuhtide TOP 20. Riigi- ja kohaliku omavalitsuse asutuste eelarve TOP 20. Riigi- ja kohaliku omavalitsuse asutuste töötajate arvu TOP 20. Naistegevjuhtidega firmade osakaal 2001. aastal. Riigi- ja kohaliku omavalitsuse asutuste naisjuhtide aastapalga TOP 20. Kommenteerib Tiina Raitviir.

  6. MCSA Windows Server 2012 R2 complete study guide exams 70-410, 70-411, 70-412, and 70-417

    CERN Document Server

    Panek, William

    2014-01-01

    Prepare for the MCSA Windows Server 2012 R2 Exams Microsoft's new version of the MCSA certification for WindowsServer 2012 R2 requires passing three exams (or one Upgrade exam ifyou have your MCSA or MCITP in Windows Server 2008). Thisvalue-priced study guide includes more than 1,000 pages of qualityexam-prep content, covering 100% of the objective domains of allthree exams (as well as the Upgrade exam, 70-417). In addition, you get access to an interactive practice testenvironment with more than 500 questions, electronic flashcards,and videos showing how to perform the more difficult tasks.

  7. Hot Ductility Characterization of Sanicro-28 Super-Austenitic Stainless Steel

    Science.gov (United States)

    Mirzaei, A.; Zarei-Hanzaki, A.; Abedi, H. R.

    2016-05-01

    The hot ductility behavior of a super-austenitic stainless steel has been studied using tensile testing method in the temperature range from 1073 K to 1373 K (800 °C to 1100 °C) under the strain rates of 0.1, 0.01, and 0.001 s-1. The hot compression tests were also performed at the same deformation condition to identify the activated restoration mechanisms. At lower temperatures [ i.e., 1073 K and 1173 K (800 °C and 900 °C)], the serration of initial grain boundaries confirms the occurrence of dynamic recovery as the predominant restoration process. However, in the course of applied deformation, the initial microstructure is recrystallized at higher temperatures [ i.e., 1273 K and 1373 K (1000 °C and 1100 °C)]. In this respect, annealing the twin boundaries could well stimulate the recrystallization kinetic through initiation new annealing twins on prior annealing twin boundaries. The hot tensile results show that there is a general trend of increasing ductility by temperature. However, two regions of ductility drop are recognized at 1273 K and 1373 K (1000°C)/0.1s-1 and (1100°C)/0.01s-1. The ductility variations at different conditions of temperature and strain rate are discussed in terms of simultaneous activation of grain boundary sliding and restoration processes. The observed ductility troughs are attributed to the occurrence of grain boundary sliding and the resulting R-type and W-type cracks. The occurrence of dynamic recrystallization is also considered as the main factor increasing the ductility at higher temperatures. The enhanced ductility is primarily originated from the post-uniform elongation behavior, which is directly associated with the strain rate sensitivity of the experimental material.

  8. Evolution of the cyclic plastic response of Sanicro 25 steel cycled at ambient and elevated temperatures

    Czech Academy of Sciences Publication Activity Database

    Polák, Jaroslav; Petráš, Roman; Heczko, Milan; Kruml, Tomáš; Chai, G.

    2016-01-01

    Roč. 83, FEB (2016), s. 75-83 ISSN 0142-1123 R&D Projects: GA ČR(CZ) GA13-23652S Institutional support: RVO:68081723 Keywords : Cyclic plastic ity * Hysteresis loop analysis * Heat resistant steel * Dislocation structure * Effect of temperature Subject RIV: JL - Materials Fatigue, Friction Mechanics Impact factor: 2.899, year: 2016

  9. 48 CFR 1852.211-70 - Packaging, handling, and transportation.

    Science.gov (United States)

    2010-10-01

    ... transportation. 1852.211-70 Section 1852.211-70 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND... and Clauses 1852.211-70 Packaging, handling, and transportation. As prescribed in 1811.404-70, insert the following clause: Packaging, Handling, and Transportation (SEPT 2005) (a) The Contractor shall...

  10. 48 CFR 615.205-70 - Use of English language.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Use of English language. 615.205-70 Section 615.205-70 Federal Acquisition Regulations System DEPARTMENT OF STATE CONTRACTING... Information 615.205-70 Use of English language. The requirements of DOSAR 614.201-70 also apply when...

  11. Expo 70 - Osaka, Japón

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1970-06-01

    Full Text Available The following pavillions at the Expo-70 in Osaka are outstanding. The West German Exhibit, with a structure reminiscent of an astronomical observatory. The U.R.R.S. Pavillion, which is 109 m high, and has a tubular support. The Motor Car Industry Display, which involves two units and a tent like roof. The Festival Palace, within the representative zone of the Exhibition, and houses the official ceremonies in honour of the participating nations. The Tashida IHI Pavillion, whose structure includes the use of 1,338 interconnected tetrapods. The Takara Group Display, which is an abstract concept, consisting of a large number of cube space forms. The firm Takenaka Komuten Co. Ltd. has taken a very active part in the construction of 28 buildings, both Japanese and foreign.Destacan en la EXPO-70 los siguientes pabellones: el pabellón de la Alemania Occidental, con su estructura en forma de cúpula para observaciones astronómicas; el de la U.R.S.S., de 109 m de altura y estructura tubular; el de la Industria Automovilística, que consta de dos unidades y presenta una cubierta en forma de tienda de campaña; el palacio de Festivales, que forma parte de la zona representativa de la exposición; en él se desarrollan los actos dedicados a las naciones participantes; el pabellón Toshiba IHI, en cuya estructura se emplearon un total de 1.338 tetrápodos ensamblados; el pabellón del grupo Takara, diseñado con una concepción abstracta —a base de cubos—. La empresa «Takenaka Komuten, Co. Ltd.», ha intervenido eficazmente en la construcción de 28 pabellones, japoneses y extranjeros.

  12. 70th birthday of Reinhard Folk

    Directory of Open Access Journals (Sweden)

    Editorial Board

    2015-06-01

    Full Text Available On April 29, 2015 Reinhard Folk - member of the Editorial Board of "Condensed Matter Physics", renowned expert in the fields of condensed matter physics, phase transitions and critical phenomena - celebrated his 70th birthday. Reinhard Folk was born in Neuendettelsau, Germany. He studied at the University of Vienna, where in 1973 he defended his doctoral thesis "Hydrodynamic Equations of Dielectric Crystals" (under supervision of Franz Schwabl. In the same year he started working at the Institute for Theoretical Physics, Johannes Kepler University in Linz, at first as assistant, later as an associate professor, then as extraordinary professor, and finally as Director of the research group "Phase Transitions and Critical Phenomena". His research interests cover various fields of condensed matter physics. In particular, he obtained important results in the theory of ferroelectrics, quantum liquids, critical phenomena in magnets and random systems, spin liquids, superconductors, and neural networks. Reinhard Folk and his collaborators performed a series of studies that became the basis for understanding and quantitative description of phenomena occurring in many systems. Included amongst these are the description of the thermodynamic properties of matter in the vicinity of Lifshitz points, the description of critical dynamics in systems with different types of conservation laws, the analysis of effective (non-asymptotic critical behaviour, and generalized hydrodynamics of many-particle systems. Computing algorithms, resummation methods of asymptotic perturbation series proposed by Reinhard Folk and his collaborators, cover a wide range of applications. In 1982 (together with Volker Dohm he was awarded the Walter Schottky Prize of the German Physical Society for his studies of the critical dynamics of helium-4 . Those of us who were fortunate to know Reinhard Folk closer, are aware of his interests in the history of culture and the history of

  13. Robotic liver surgery: results for 70 resections.

    Science.gov (United States)

    Giulianotti, Pier Cristoforo; Coratti, Andrea; Sbrana, Fabio; Addeo, Pietro; Bianco, Francesco Maria; Buchs, Nicolas Christian; Annechiarico, Mario; Benedetti, Enrico

    2011-01-01

    Robotic surgery is gaining popularity for digestive surgery; however, its use for liver surgery is reported scarcely. This article reviews a surgeon's experience with the use of robotic surgery for liver resections. From March 2002 to March 2009, 70 robotic liver resections were performed at 2 different centers by a single surgeon. The surgical procedure and postoperative outcome data were reviewed retrospectively. Malignant tumors were indications for resections in 42 (60%) patients, whereas benign tumors were indications in 28 (40%) patients. The median age was 60 years (range, 21-84) and 57% of patients were female. Major liver resections (≥ 3 liver segments) were performed in 27 (38.5%) patients. There were 4 conversions to open surgery (5.7%). The median operative time for a major resection was 313 min (range, 220-480) and 198 min (range, 90-459) for minor resection. The median blood loss was 150 mL (range, 20-1,800) for minor resection and 300 mL (range, 100-2,000) for major resection. The mortality rate was 0%, and the overall rate of complications was 21%. Major morbidity occurred in 4 patients in the major hepatectomies group (14.8%) and in 4 patients in the minor hepatectomies group (9.3%). All complications were managed conservatively and none required reoperation. This preliminary experience shows that robotic surgery can be used safely for liver resections with a limited conversion rate, blood loss, and postoperative morbidity. Robotics offers a new technical option for minimally invasive liver surgery. Copyright © 2011 Mosby, Inc. All rights reserved.

  14. Reduced Hsp70 and Glutamine in Pediatric Severe Malaria Anemia

    DEFF Research Database (Denmark)

    Kempaiah, Prakasha; Dokladny, Karol; Karim, Zachary

    2016-01-01

    by decreased HSPA1A, a heat shock protein (Hsp) 70 coding gene. Hsp70 is a ubiquitous chaperone that regulates Nuclear Factor-kappa B (NF-κB) signaling and production of pro-inflammatory cytokines known to be important in malaria pathogenesis (e.g., IL-1β, IL-6 and TNF-α). Since the role of host Hsp70...... in malaria pathogenesis is unexplored, we investigated Hsp70 and molecular pathways in children with SMA. Validation experiments revealed that leukocytic HSP70 transcripts were reduced in SMA relative to non-severe malaria, and that intraleukocytic hemozoin (PfHz) was associated with lower HSP70. HSP70...... was correlated with reticulocyte production and Hb. Since glutamine (Gln) up-regulates Hsp70, modulates NF-κB activation, and attenuates over-expression of pro-inflammatory cytokines, circulating Gln was measured in children with malaria. Reduced Gln was associated with increased risk of developing SMA...

  15. Physics design of a 70 MeV high intensity cyclotron, CYCIAE-70

    International Nuclear Information System (INIS)

    Zhang Tianjue; An Shizhong; Wang Chuan; Yin Zhiguo; Wei Sumin; Li Ming; Yang Jianjun; Ji Bin; Jia Xianlu; Zhong Junqing; Yang Fang

    2011-01-01

    This paper introduces the physics design of a 70 MeV high intensity cyclotron at China Institute of Atomic Energy (CIAE), which is aimed for multiple uses including radioactive ion-beam (RIB) production. The machine adopts a compact structure of four straight sectors, capable of accelerating two kinds of beams, i.e. H − and D − . The proton and deuteron beam will be extracted in dual opposite directions by charge exchange stripping devices. The energy of the extracted proton beam is in the range 35–70 MeV with an intensity up to 700 μA. The corresponding values for the deuteron beam are 18–33 MeV and 40 μA. This paper will present the main characteristics and parameters in the design of the 70 MeV cyclotron, the results of the basic beam dynamics study, as well as the physics in the design of the different systems, including the main magnet, RF, injection and extraction systems, etc.

  16. World population trends in 1960-70.

    Science.gov (United States)

    Tabah, L; Kono, S

    1974-01-01

    The rapid rise in world population for the 4 periods 1950-1955, 1955 -1960, 1960-1965, and 1965-1970 is mainly due to a decline in the crude death rate from 24/1000 in 1950-1955 to 16.1/1000 in 1965-1970. 1950 was a turning-point in mortality rates because for the first time public health measures became widely available in less developed regions. These less developed regions contained 63.4% of the world population in 1930, 65.5% in 1950, and 70.0% in 1970. World population increased by 22% in 1960-1970, compared to 20% in 1950-1960, which was twice as much as the 20% registered for the 20 years 1930-1950. Developed regions contributed a 14% increase between 1950-1960 and 12% between 1960-1970; in less developed regions the increases were 23% and 27% respectively. Demographic conferences have begun to neglect mortality, but this is still a vital part of total population growth. Evidence is that the mortality decline in less developed countries is beginning to level off so that previous population projections may not be reached. What decline has been seen in crude birth rates in less developed countries is largely attributable to declines in East Asia, notably in the People's Republic of China, Republic of Korea, and other Chinese or Chinese culture-related populations, as well as some of the smaller Latin American countries and some small islands off Africa. Such demographic giants as India, Indonesia, Pakistan, and sub-Saharan Africa have shown little change. In areas with high fertility there are more mothers under age 20 and more births to women over age 35 while in low-fertility areas births are concentrated in the 20-35 year groups. An interesting example is Japan which has 50% of its births to mothers 25-29 years of age. Less developed countries have a larger proportion o f the population under age 14 while developed countries which have had lower fertility for a number of years have larger numbers in the older age groups. In less developed areas the greatest

  17. 48 CFR 1552.217-70 - Evaluation of contract options.

    Science.gov (United States)

    2010-10-01

    ... options. 1552.217-70 Section 1552.217-70 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION... 1552.217-70 Evaluation of contract options. As prescribed in 1517.208(a), insert the following solicitation provision in Requests for Proposals when the solicitation contains options. Evaluation of Contract...

  18. 7 CFR 2.70 - Deputy Chief Economist.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Deputy Chief Economist. 2.70 Section 2.70 Agriculture... GENERAL OFFICERS OF THE DEPARTMENT Delegations of Authority by the Chief Economist § 2.70 Deputy Chief Economist. Pursuant to § 2.29, the following delegation of authority is made by the Chief Economist to the...

  19. 46 CFR 56.50-70 - Gasoline fuel systems.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Gasoline fuel systems. 56.50-70 Section 56.50-70... APPURTENANCES Design Requirements Pertaining to Specific Systems § 56.50-70 Gasoline fuel systems. (a) Material.... Outlets in fuel lines for drawing gasoline for any purpose are prohibited. Valved openings in the bottom...

  20. 33 CFR 118.70 - Lights on swing bridges.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Lights on swing bridges. 118.70 Section 118.70 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES BRIDGE LIGHTING AND OTHER SIGNALS § 118.70 Lights on swing bridges. (a) Swing span lights on through bridges. Each...

  1. 29 CFR 70.54 - Employee Benefits Security Administration.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Employee Benefits Security Administration. 70.54 Section 70... Records and Filings § 70.54 Employee Benefits Security Administration. (a) The annual financial reports (Form 5500) and attachments/schedules as filed by employee benefit plans under the Employee Retirement...

  2. 46 CFR 78.47-70 - Portable magazine chests.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Portable magazine chests. 78.47-70 Section 78.47-70... Fire and Emergency Equipment, Etc. § 78.47-70 Portable magazine chests. (a) Portable magazine chest shall be marked in letters of at least 3 inches high “PORTABLE MAGAZINE CHEST—FLAMMABLE—KEEP LIGHTS AND...

  3. 33 CFR 1.07-70 - Right to appeal.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Right to appeal. 1.07-70 Section 1.07-70 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY GENERAL GENERAL PROVISIONS Enforcement; Civil and Criminal Penalty Proceedings § 1.07-70 Right to appeal. (a) Any appeal from...

  4. 48 CFR 2052.242-70 - Resolving differing professional views.

    Science.gov (United States)

    2010-10-01

    ... resolution of differing professional views (DPVs) of health and safety related concerns associated with the... professional views. 2052.242-70 Section 2052.242-70 Federal Acquisition Regulations System NUCLEAR REGULATORY....242-70 Resolving differing professional views. As prescribed in 2042.570-1, the contracting officer...

  5. 27 CFR 70.82 - Payment on notice and demand.

    Science.gov (United States)

    2010-04-01

    ... demand. 70.82 Section 70.82 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... Collection of Excise and Special (Occupational) Tax Notice and Demand § 70.82 Payment on notice and demand. Upon receipt of notice and demand from the appropriate TTB officer, there shall be paid at the place...

  6. 7 CFR 1209.70 - Right of the Secretary.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Right of the Secretary. 1209.70 Section 1209.70..., AND CONSUMER INFORMATION ORDER Mushroom Promotion, Research, and Consumer Information Order Miscellaneous § 1209.70 Right of the Secretary. All fiscal matters, programs, plans, or projects, rules or...

  7. 7 CFR 1215.70 - Right of the Secretary.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Right of the Secretary. 1215.70 Section 1215.70... CONSUMER INFORMATION Popcorn Promotion, Research, and Consumer Information Order Miscellaneous § 1215.70 Right of the Secretary. All fiscal matters, programs, plans, or projects, contracts, rules or...

  8. 50 CFR 70.9 - Wildlife species management.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Wildlife species management. 70.9 Section 70.9 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) MANAGEMENT OF FISHERIES CONSERVATION AREAS NATIONAL FISH HATCHERIES § 70.9 Wildlife species...

  9. 48 CFR 1323.404-70 - DOC affirmative procurement program.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false DOC affirmative procurement program. 1323.404-70 Section 1323.404-70 Federal Acquisition Regulations System DEPARTMENT OF..., OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Use of Recovered Materials 1323.404-70 DOC affirmative procurement...

  10. 27 CFR 70.52 - Signature presumed authentic.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Signature presumed authentic. 70.52 Section 70.52 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... Collection of Excise and Special (Occupational) Tax Collection-General Provisions § 70.52 Signature presumed...

  11. 21 CFR 11.70 - Signature/record linking.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Signature/record linking. 11.70 Section 11.70 Food... RECORDS; ELECTRONIC SIGNATURES Electronic Records § 11.70 Signature/record linking. Electronic signatures and handwritten signatures executed to electronic records shall be linked to their respective...

  12. 28 CFR 70.45 - Cost and price analysis.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Cost and price analysis. 70.45 Section 70... NON-PROFIT ORGANIZATIONS Post-Award Requirements Procurement Standards § 70.45 Cost and price analysis. Some form of cost or price analysis must be made and documented in the procurement files in connection...

  13. 7 CFR 70.24 - Financial interest of graders.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Financial interest of graders. 70.24 Section 70.24 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... Graders § 70.24 Financial interest of graders. Graders shall not render service on any product in which...

  14. 42 CFR 440.70 - Home health services.

    Science.gov (United States)

    2010-10-01

    ... an acute illness to avoid the recipient's transfer to a nursing facility. (d) “Home health agency... 42 Public Health 4 2010-10-01 2010-10-01 false Home health services. 440.70 Section 440.70 Public...) MEDICAL ASSISTANCE PROGRAMS SERVICES: GENERAL PROVISIONS Definitions § 440.70 Home health services. (a...

  15. 50 CFR 260.70 - Schedule of fees.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Schedule of fees. 260.70 Section 260.70... Products for Human Consumption Fees and Charges § 260.70 Schedule of fees. (a) Unless otherwise provided in a written agreement between the applicant and the Secretary, the fees to be charged and collected...

  16. 42 CFR 494.70 - Condition: Patients' rights.

    Science.gov (United States)

    2010-10-01

    ... patient for ESRD of his or her own medical status as documented in the patient's medical record, unless... 42 Public Health 5 2010-10-01 2010-10-01 false Condition: Patients' rights. 494.70 Section 494.70... Patient Care § 494.70 Condition: Patients' rights. The dialysis facility must inform patients (or their...

  17. 48 CFR 1252.237-70 - Qualifications of contractor employees.

    Science.gov (United States)

    2010-10-01

    ... contractor employees. 1252.237-70 Section 1252.237-70 Federal Acquisition Regulations System DEPARTMENT OF....237-70 Qualifications of contractor employees. As prescribed in (TAR) 48 CFR 1237.110(a), insert the following clause: Qualifications of Contractor Employees (APR 2005) a. Definitions. As used in this clause...

  18. 30 CFR 70.202 - Certified person; sampling.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Certified person; sampling. 70.202 Section 70... HEALTH MANDATORY HEALTH STANDARDS-UNDERGROUND COAL MINES Sampling Procedures § 70.202 Certified person; sampling. (a) The respirable dust sampling required by this part shall be done by a certified person. (b...

  19. 28 CFR 544.70 - Purpose and scope.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Purpose and scope. 544.70 Section 544.70... Literacy Program § 544.70 Purpose and scope. Except as provided for in § 544.71, an inmate confined in a federal institution who does not have a verified General Educational Development (GED) credential or high...

  20. 29 CFR 801.70 - Implementation by the Secretary.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Implementation by the Secretary. 801.70 Section 801.70 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR OTHER LAWS... Vacation of Decision and Order of Administrative Law Judge § 801.70 Implementation by the Secretary. (a...

  1. 45 CFR 7.0 - Who are employees.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Who are employees. 7.0 Section 7.0 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION EMPLOYEE INVENTIONS § 7.0 Who are employees. As used in this part, the term Government employee means any officer or employee, civilian or...

  2. 29 CFR 452.70 - Contents of literature.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 2 2010-07-01 2010-07-01 false Contents of literature. 452.70 Section 452.70 Labor... DISCLOSURE ACT OF 1959 Campaign Safeguards § 452.70 Contents of literature. The Act does not and unions may not regulate the contents of campaign literature which candidates may wish to have distributed by the...

  3. 21 CFR 26.70 - Conformity assessment bodies.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Conformity assessment bodies. 26.70 Section 26.70...Frameworkâ Provisions § 26.70 Conformity assessment bodies. Each party recognizes that the conformity... conformity in relation to its requirements as specified in subpart B of this part. The parties shall specify...

  4. 28 CFR 70.73 - Collection of amounts due.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Collection of amounts due. 70.73 Section 70.73 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) UNIFORM ADMINISTRATIVE REQUIREMENTS... OTHER NON-PROFIT ORGANIZATIONS After-the-Award Requirements § 70.73 Collection of amounts due. (a) Any...

  5. 48 CFR 847.306-70 - Transportation payment and audit.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Transportation payment and audit. 847.306-70 Section 847.306-70 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS CONTRACT MANAGEMENT TRANSPORTATION Transportation in Supply Contracts 847.306-70 Transportation payment and audit. Transportation payments ar...

  6. 46 CFR 133.70 - Personal lifesaving appliances.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Personal lifesaving appliances. 133.70 Section 133.70 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS LIFESAVING SYSTEMS Requirements for All OSVs § 133.70 Personal lifesaving appliances. (a) Lifebuoys. Each OSV must...

  7. The Complex Function of Hsp70 in Metastatic Cancer

    Energy Technology Data Exchange (ETDEWEB)

    Juhasz, Kata; Lipp, Anna-Maria; Nimmervoll, Benedikt; Sonnleitner, Alois; Hesse, Jan; Haselgruebler, Thomas; Balogi, Zsolt, E-mail: zsolt.balogi@cbl.at [Center for Advanced Bioanalysis GmbH, Gruberstr. 40-42, A-4020 Linz (Austria)

    2013-12-20

    Elevated expression of the inducible heat shock protein 70 (Hsp70) is known to correlate with poor prognosis in many cancers. Hsp70 confers survival advantage as well as resistance to chemotherapeutic agents, and promotes tumor cell invasion. At the same time, tumor-derived extracellular Hsp70 has been recognized as a “chaperokine”, activating antitumor immunity. In this review we discuss localization dependent functions of Hsp70 in the context of invasive cancer. Understanding the molecular principles of metastasis formation steps, as well as interactions of the tumor cells with the microenvironment and the immune system is essential for fighting metastatic cancer. Although Hsp70 has been implicated in different steps of the metastatic process, the exact mechanisms of its action remain to be explored. Known and potential functions of Hsp70 in controlling or modulating of invasion and metastasis are discussed.

  8. Cytosolic Hsp70/Hsc70 protein expression in lymphocytes exposed to low gamma-ray dose

    International Nuclear Information System (INIS)

    Manzanares A, E.; Vega C, H.R.; Letechipia de Leon, C.; Guzman E, L.J.; Garcia T, M.

    2004-01-01

    The purpose of this study was to evaluate the effect of low gamma ray intensity upon Hsp70 expression in human Iymphocytes. The heat shock proteins (Hsp) family, are a group of proteins present in all living organism, therefore there are highly conserved and are related to adaptation and evolution. At cellular level these proteins acts as chaperones correcting denatured proteins. When a stress agent, such heavy metals, UV, heat, etc. is affecting a cell a response to this aggression is triggered only through over expression of Hsp. Several studies has been carried out in which the cellular effect are observed, mostly of these studies uses large doses, but very few studies are related with low doses. Blood of healthy volunteers was obtained and the Iymphocytes were isolated by ficoll- histopaque gradient. Experimental lots were irradiated in a 137 Cs gamma-ray. Hsp70 expression was found since 0.5 c Gy, indicating a threshold to very low doses of gamma rays. (Author)

  9. Cytosolic Hsp70/Hsc70 protein expression in lymphocytes exposed to low gamma-ray dose

    Energy Technology Data Exchange (ETDEWEB)

    Manzanares A, E.; Vega C, H.R.; Letechipia de Leon, C. [Unidades Academicas de Estudios Nucleares, UAZ, A.P. 336, 98000 Zacatecas (Mexico)]. E-mail: emanz@cantera.reduaz.mx; Guzman E, L.J. [Unidad Academica de Biologia Experimental, Guadalupe, Zacatecas (Mexico); Garcia T, M. [LIBRA, Centro I and D, Campus Miguel Delibes, Valladolid 47011 (Spain)

    2004-07-01

    The purpose of this study was to evaluate the effect of low gamma ray intensity upon Hsp70 expression in human Iymphocytes. The heat shock proteins (Hsp) family, are a group of proteins present in all living organism, therefore there are highly conserved and are related to adaptation and evolution. At cellular level these proteins acts as chaperones correcting denatured proteins. When a stress agent, such heavy metals, UV, heat, etc. is affecting a cell a response to this aggression is triggered only through over expression of Hsp. Several studies has been carried out in which the cellular effect are observed, mostly of these studies uses large doses, but very few studies are related with low doses. Blood of healthy volunteers was obtained and the Iymphocytes were isolated by ficoll- histopaque gradient. Experimental lots were irradiated in a {sup 137} Cs gamma-ray. Hsp70 expression was found since 0.5 c Gy, indicating a threshold to very low doses of gamma rays. (Author)

  10. Imbalance of Hsp70 family variants fosters tau accumulation.

    Science.gov (United States)

    Jinwal, Umesh K; Akoury, Elias; Abisambra, Jose F; O'Leary, John C; Thompson, Andrea D; Blair, Laura J; Jin, Ying; Bacon, Justin; Nordhues, Bryce A; Cockman, Matthew; Zhang, Juan; Li, Pengfei; Zhang, Bo; Borysov, Sergiy; Uversky, Vladimir N; Biernat, Jacek; Mandelkow, Eckhard; Gestwicki, Jason E; Zweckstetter, Markus; Dickey, Chad A

    2013-04-01

    Dysfunctional tau accumulation is a major contributing factor in tauopathies, and the heat-shock protein 70 (Hsp70) seems to play an important role in this accumulation. Several reports suggest that Hsp70 proteins can cause tau degradation to be accelerated or slowed, but how these opposing activities are controlled is unclear. Here we demonstrate that highly homologous variants in the Hsp70 family can have opposing effects on tau clearance kinetics. When overexpressed in a tetracycline (Tet)-based protein chase model, constitutive heat shock cognate 70 (Hsc70) and inducible Hsp72 slowed or accelerated tau clearance, respectively. Tau synergized with Hsc70, but not Hsp72, to promote microtubule assembly at nearly twice the rate of either Hsp70 homologue in reconstituted, ATP-regenerating Xenopus extracts supplemented with rhodamine-labeled tubulin and human recombinant Hsp72 and Hsc70. Nuclear magnetic resonance spectroscopy with human recombinant protein revealed that Hsp72 had greater affinity for tau than Hsc70 (I/I0 ratio difference of 0.3), but Hsc70 was 30 times more abundant than Hsp72 in human and mouse brain tissue. This indicates that the predominant Hsp70 variant in the brain is Hsc70, suggesting that the brain environment primarily supports slower tau clearance. Despite its capacity to clear tau, Hsp72 was not induced in the Alzheimer's disease brain, suggesting a mechanism for age-associated onset of the disease. Through the use of chimeras that blended the domains of Hsp72 and Hsc70, we determined that the reason for these differences between Hsc70 and Hsp72 with regard to tau clearance kinetics lies within their C-terminal domains, which are essential for their interactions with substrates and cochaperones. Hsp72 but not Hsc70 in the presence of tau was able to recruit the cochaperone ubiquitin ligase CHIP, which is known to facilitate the ubiquitination of tau, describing a possible mechanism of how the C-termini of these homologous Hsp70 variants

  11. Circulating Heat Shock Protein 70 in Health, Aging and Disease

    Directory of Open Access Journals (Sweden)

    Demanet Christian

    2011-03-01

    Full Text Available Abstract Background Heat shock proteins (Hsp are ubiquitously synthesised in virtually all species and it is hypothesised that they might have beneficial health effects. Recent studies have identified circulating Hsp as an important mediator in inflammation - the effects of low-grade inflammation in the aging process are overwhelming. While much is known about intracellular Hsp70, scant data exist on circulating Hsp70 in the aging context. Therefore, the objectives of this study were to investigate the effect of age and disease on circulating Hsp70 and, in particular, to evaluate the association between circulating Hsp70 and inflammatory parameters. Results Serum Hsp70, Interleukin (IL -10, IL-6 and Tumor Necrosis Factor (TNF alpha concentrations were determined in 90 hospitalised geriatric patients (aged 83 ± 6 years and in 200 community-dwelling control subjects (100 elderly, aged 74 ± 5 years, and 100 young, aged 23 ± 3 years. In the community-dwelling elderly, serum Hsp70 and IL-10 concentrations were significantly lower and IL-6 was significantly higher when compared to healthy young control subjects. Elderly patients presenting inflammation (CRP serum levels ≥5 mg/L showed significantly (p = 0.007 higher Hsp70 values; and Hsp70 correlated positively (p Conclusions The present data provide new evidence that serum concentration of Hsp70 decreases with age in a normal population. Our study also shows that higher levels of Hsp70 are associated with inflammation and frailty in elderly patients.

  12. Molecular cloning and expression of a heat-shock cognate 70 (hsc70) gene from swordtail fish ( Xiphophorus helleri)

    Science.gov (United States)

    Li, Ningqiu; Fu, Xiaozhe; Han, Jingang; Shi, Cunbin; Huang, Zhibin; Wu, Shuqin

    2013-07-01

    Heat shock proteins are a family of molecular chaperones that are involved in many aspects of protein homeostasis. In the present study, a full-length cDNA, encoding the constitutively expressed 70-kDa heat shock cognate protein (Hsc70), was isolated from swordtail fish ( Xiphophorus helleri) and designated as XheHsc70. The Xhehsc70 cDNA was 2 104 bp long with an open reading frame of 1 941 bp, and it encoded a protein of 646 amino acids with a theoretical molecular weight of 70.77 kDa and an isoelectric point of 5.04. The deduced amino acid sequence shared 94.1%-98.6% identities with the Hsc70s from a number of other fish species. Tissue distribution results show that the Xhehsc70 mRNA was expressed in brain, heart, head kidney, kidney, spleen, liver, muscle, gill, and peripheral blood. After immunization with formalin-killed Vibrio alginolyticus cells there was a significant increase in the Xhehsc70 mRNA transcriptional level in the head kidney of the vaccinated fish compared with in the control at 6, 12, 24, and 48 h as shown by quantitative real time RT-PCR. Based on an analysis of the amino acid sequence of XheHsc70, its phylogeny, and Xhehsc70 mRNA expression, XheHsc70 was identified as a member of the cytoplasmic Hsc70 (constitutive) subfamily of the Hsp70 family of heat shock proteins, suggesting that it may play a role in the immune response. The Xhehsc70 cDNA sequence reported in this study was submitted to GenBank under the accession number JF739182.

  13. SWAP-70 contributes to spontaneous transformation of mouse embryo fibroblasts

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Yu-Tzu; Shu, Chung-Li; Lai, Jing-Yang; Lin, Ching-Yu; Chuu, Chih-Pin [Institute of Cellular and System Medicine National Health Research Institute, Zhunan Town 35053, Miaoli County, Taiwan, ROC (China); Morishita, Kazuhiro; Ichikawa, Tomonaga [Division of Tumor and Cellular Biochemistry Department of Medical Sciences Faculty of Medicine University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki-shi, Miyazaki 889-1692 Japan (Japan); Jessberger, Rolf [Faculty of Medicine Carl Gustav Carus, Institute of Physiological Chemistry, Dresden University of Technology, Dresden (Germany); Fukui, Yasuhisa, E-mail: 990412@nhri.org.tw [Institute of Cellular and System Medicine National Health Research Institute, Zhunan Town 35053, Miaoli County, Taiwan, ROC (China)

    2016-07-15

    Mouse embryo fibroblasts (MEFs) grow slowly after cultivation from animals, however, after an extended period of cultivation, their growth accelerates. We found that SWAP-70 deficient MEFs failed to increase growth rates. They maintain normal growth rates and proliferation cycles for at least 5 years. Complementing SWAP-70 deficiency in one of these MEF clones, MEF1F2, by expressing human SWAP-70 resulted in fast growth of the cells after further cultivation for a long period. The resulting cells show a transformation phenotype, since they grow on top of each other and do not show contact inhibition. This phenotype was reverted when sanguinarine, a putative SWAP-70 inhibitor, was added. Two SWAP-70 expressing clones were examined in detail. Even after cell density became very high their cdc2 and NFκB were still activated suggesting that they do not stop growing. One of the clones formed colonies in soft agar and formed tumors in nude mice. Lately, one more clone became transformed being able to make colonies in soft agar. We maintain 4 human SWAP-70 expressing MEF1F2 cell lines. Three out of 4 clones exhibited transforming phenotypes. The mouse SWAP-70 gene also promoted transformation of MEFs. Taken together our data suggest that SWAP-70 is not a typical oncogene, but is required for spontaneous transformation of MEFs. - Highlights: • Mouse embryo fibroblasts (MEFs) lacking SWAP-70 do not cause spontaneous transform. • Adding back of SWAP-70 to SWAP-70-deficient MEFs induces spontaneous transformation. • SWAP-70 is required for spontaneous transformation of MEFs.

  14. Kinode suurmood on 70-ndate verine filmikunst / Andris Feldmanis

    Index Scriptorium Estoniae

    Feldmanis, Andris, 1982-

    2007-01-01

    Filmiprojekt "Grindhouse", mis koosneb Robert Rodrigueze retroõudukast "Planet Terror" ja Quentin Tarantino 70-ndatest inspireeritud õudusfilmist "Surmakindel" ("Death Proof") : Ameerika Ühendriigid 2007

  15. 21 CFR 70.19 - Fees for listing.

    Science.gov (United States)

    2010-04-01

    ... order, bank draft or certified check drawn to the order of the Food and Drug Administration, collectable... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Fees for listing. 70.19 Section 70.19 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL COLOR ADDITIVES...

  16. 48 CFR 852.273-70 - Late offers.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Late offers. 852.273-70... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses 852.273-70 Late offers. As prescribed in 873.110(a), insert the following provision: Late Offers (JAN 2003) This provision replaces...

  17. 45 CFR 201.70 - Treatment of replacement checks.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Treatment of replacement checks. 201.70 Section... STATES FOR PUBLIC ASSISTANCE PROGRAMS Review and Audits § 201.70 Treatment of replacement checks. (a... (FFP) for replacement checks under titles I, VI-A, X, XIV, XVI (AABD) except under the circumstances...

  18. Asteroseismic observations and modelling of 70 Ophiuchi AB

    Energy Technology Data Exchange (ETDEWEB)

    Eggenberger, P; Miglio, A [Institut d' Astrophysique et de Geophysique de l' Universite de Liege, 17 Allee du 6 Aout, B-4000 Liege (Belgium); Carrier, F [Institute of Astronomy, University of Leuven, Celestijnenlaan 200 D, B-3001 Leuven (Belgium); Fernandes, J [Observatorio Astronomico da Universidade de Coimbra e Departamento de Matematica, FCTUC (Portugal); Santos, N C [Centro de AstrofIsica, Universidade do Porto, Rua das Estrelas, P-4150-762 Porto (Portugal)], E-mail: eggenberger@astro.ulg.ac.be

    2008-10-15

    The analysis of solar-like oscillations for stars belonging to a binary system provides an opportunity to probe the internal stellar structure and to test our knowledge of stellar physics. We present asteroseismic observations of 70 Oph A performed with the HARPS spectrograph together with a comprehensive theoretical calibration of the 70 Ophiuchi system.

  19. 48 CFR 1239.70 - Solicitation provision and contract clause.

    Science.gov (United States)

    2010-10-01

    ... 1252.239-71, Information Technology Security Plan and Accreditation, and the clause at (TAR) 48 CFR 1252.239-70, Security Requirements for Unclassified Information Technology Resources, in all... TRANSPORTATION SPECIAL CATEGORIES OF CONTRACTING ACQUISITION OF INFORMATION TECHNOLOGY General 1239.70...

  20. 48 CFR 3452.247-70 - Foreign travel.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Foreign travel. 3452.247-70 Section 3452.247-70 Federal Acquisition Regulations System DEPARTMENT OF EDUCATION ACQUISITION... travel” means travel outside the fifty States comprising the United States, the District of Columbia, and...

  1. 28 CFR 549.70 - Purpose and scope.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Purpose and scope. 549.70 Section 549.70 Judicial Administration BUREAU OF PRISONS, DEPARTMENT OF JUSTICE INSTITUTIONAL MANAGEMENT MEDICAL SERVICES...) Are found responsible through the Disciplinary Hearing Process to have injured an inmate who, as a...

  2. 48 CFR 632.702-70 - DOS policy.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false DOS policy. 632.702-70... REQUIREMENTS CONTRACT FINANCING Contract Funding 632.702-70 DOS policy. The Department's policy is to provide... incremental funding of cost-reimbursement contracts. Fixed-price, labor-hour, and time-and-materials contracts...

  3. 48 CFR 452.247-70 - Delivery Location.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Delivery Location. 452.247-70 Section 452.247-70 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE CLAUSES AND... Location. As prescribed in 447.302, insert a clause substantially as follows: Delivery Location (FEB 1988...

  4. 41 CFR 105-70.019 - Prehearing conferences.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Prehearing conferences. 105-70.019 Section 105-70.019 Public Contracts and Property Management Federal Property Management... party chooses to waive appearance at an oral hearing and to submit only documentary evidence (subject to...

  5. 40 CFR 70.11 - Requirements for enforcement authority.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 15 2010-07-01 2010-07-01 false Requirements for enforcement authority. 70.11 Section 70.11 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR...) Burden of proof. The burden of proof and degree of knowledge or intent required under State law for...

  6. 48 CFR 952.247-70 - Foreign travel.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Foreign travel. 952.247-70... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 952.247-70 Foreign travel. As prescribed in 947.7002, insert the following clause: Foreign Travel (JUN 2010) Contractor foreign travel...

  7. 14 CFR 1260.70 - Delegation of administration.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Delegation of administration. 1260.70... AGREEMENTS General Post-Award Requirements § 1260.70 Delegation of administration. (a) If a grant or a... of the file. (4) Waiver of delegation of property administration duties that are to be instituted by...

  8. 27 CFR 70.241 - Property exempt from levy.

    Science.gov (United States)

    2010-04-01

    ... in §§ 70.242 through 70.245 of this part. (10) Certain service-connected disability payments. Any amount payable to an individual as a service-connected (within the meaning of 38 U.S.C. 101(16... such property declared to be exempt. If the taxpayer objects at the time of the seizure to the...

  9. 21 CFR 820.70 - Production and process controls.

    Science.gov (United States)

    2010-04-01

    ...) MEDICAL DEVICES QUALITY SYSTEM REGULATION Production and Process Controls § 820.70 Production and process... used as part of production or the quality system, the manufacturer shall validate computer software for... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Production and process controls. 820.70 Section...

  10. 29 CFR 70.42 - Consent to Pay Fees.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Consent to Pay Fees. 70.42 Section 70.42 Labor Office of the....42 Consent to Pay Fees. (a) The filing of a request under this subpart will be deemed to constitute an agreement by the requester to pay all applicable fees charged under this part up to and including...

  11. 27 CFR 70.97 - Failure to pay tax.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Failure to pay tax. 70.97... § 70.97 Failure to pay tax. (a) Negligence—(1) General. If any part of any underpayment (as defined in... section 6651 of the Internal Revenue Code (relating to failure to file such return or pay tax) shall be...

  12. 28 CFR 70.50 - Purpose of reports and records.

    Science.gov (United States)

    2010-07-01

    ... the recipient's financial and program performance and the necessary standard reporting forms. They... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Purpose of reports and records. 70.50... AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Reports and Records § 70.50 Purpose of...

  13. Accumulation of hepatic Hsp70 and plasma cortisol in Oreochromis ...

    African Journals Online (AJOL)

    The hepatic isoforms Hsp70, Hsp74 and Hsp76 were identified and quantified from copper exposures. Long-term DDT exposure did not result in significant induction of hepatic Hsp70. An increase in plasma cortisol concentration was associated with a decrease in heat shock protein accumulation after cadmium exposure, ...

  14. 12 CFR 652.70 - Risk-based capital level.

    Science.gov (United States)

    2010-01-01

    ... risk-based capital level is the sum of the following amounts: (a) Credit and interest rate risk. The... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Risk-based capital level. 652.70 Section 652.70 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT SYSTEM FEDERAL AGRICULTURAL MORTGAGE...

  15. 29 CFR 570.70 - Purpose and scope.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Purpose and scope. 570.70 Section 570.70 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS CHILD LABOR... “provisions of section 12 [of the Act] relating to child labor shall apply to an employee below the age of 16...

  16. 48 CFR 1252.235-70 - Research misconduct.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Research misconduct. 1252.235-70 Section 1252.235-70 Federal Acquisition Regulations System DEPARTMENT OF TRANSPORTATION CLAUSES... recommendations from the investigation phase and determining appropriate corrective actions. Complainant is the...

  17. 48 CFR 2052.235-70 - Publication of research results.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Publication of research results. 2052.235-70 Section 2052.235-70 Federal Acquisition Regulations System NUCLEAR REGULATORY... recommendations which may have regulatory implications. (c) The principal investigator(s) shall coordinate all...

  18. 48 CFR 1852.233-70 - Protests to NASA.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Protests to NASA. 1852.233... 1852.233-70 Protests to NASA. As prescribed in 1833.106-70, insert the following provision: Protests to NASA (OCT 2002) Potential bidders or offerors may submit a protest under 48 CFR part 33 (FAR part 33...

  19. 48 CFR 1852.227-70 - New technology.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true New technology. 1852.227-70... New technology. As prescribed in 1827.303-70(b), insert the following clause: New Technology (NOV 1998... administration of this New Technology clause within six months of conception and/or first actual reduction to...

  20. 48 CFR 243.204-70-2 - Price ceiling.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Price ceiling. 243.204-70..., DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT CONTRACT MODIFICATIONS Change Orders 243.204-70-2 Price ceiling. Unpriced change orders shall include a not-to-exceed price. [75 FR 48277, Aug. 10, 2010] ...

  1. 48 CFR 1852.210-70 - Brand name or equal.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Brand name or equal. 1852... 1852.210-70 Brand name or equal. As prescribed in 1810.011-70(a), insert the following provision: Brand Name or Equal (DEC 1988) (a) As used in this provision, “brand name” means identification of products...

  2. 29 CFR 1960.70 - Reporting of serious accidents.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Reporting of serious accidents. 1960.70 Section 1960.70... accidents. Agencies must provide the Office of Federal Agency Programs with a summary report of each fatal and catastrophic accident investigation. The summaries shall address the date/time of accident, agency...

  3. 19 CFR 4.70 - Public Health Service requirements.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Public Health Service requirements. 4.70 Section 4... THE TREASURY VESSELS IN FOREIGN AND DOMESTIC TRADES Foreign Clearances § 4.70 Public Health Service... Public Health Service. [T.D. 00-4, 65 FR 2874, Jan. 19, 2000] ...

  4. 48 CFR 216.104-70 - Research and development.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Research and development... Contract Types § 216.104-70 Research and development. Follow the procedures at PGI 216.104-70 for selecting the appropriate research and development contract type. [71 FR 39007, July 11, 2006] ...

  5. 41 CFR 105-70.041 - Stay pending appeal.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Stay pending appeal. 105... Administration 70-IMPLEMENTATION OF THE PROGRAM FRAUD CIVIL REMEDIES ACT OF 1986 § 105-70.041 Stay pending appeal. (a) An initial decision is stayed automatically pending disposition of a motion for reconsideration...

  6. 30 CFR 70.207 - Bimonthly sampling; mechanized mining units.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Bimonthly sampling; mechanized mining units. 70... MINE SAFETY AND HEALTH MANDATORY HEALTH STANDARDS-UNDERGROUND COAL MINES Sampling Procedures § 70.207 Bimonthly sampling; mechanized mining units. (a) Each operator shall take five valid respirable dust samples...

  7. 48 CFR 452.216-70 - Award Fee.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Award Fee. 452.216-70... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses 452.216-70 Award Fee. As prescribed in 416.405, insert a clause substantially as follows: Award Fee (FEB 1988) The amount of award fee...

  8. 48 CFR 1019.202-70-7 - Mentor firms.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Mentor firms. 1019.202-70... SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 1019.202-70-7 Mentor firms. A mentor firm may be either... developmental assistance to enhance the capabilities of protégés to perform as subcontractors. Mentors will be...

  9. 40 CFR 98.70 - Definition of source category.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Definition of source category. 98.70... (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING Ammonia Manufacturing § 98.70 Definition of source category...-based feedstock produced via steam reforming of a hydrocarbon. (b) Ammonia manufacturing processes in...

  10. 31 CFR 10.70 - Administrative Law Judge.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Administrative Law Judge. 10.70... INTERNAL REVENUE SERVICE Rules Applicable to Disciplinary Proceedings § 10.70 Administrative Law Judge. (a..., firm or other entity, or appraiser will be conducted by an Administrative Law Judge appointed as...

  11. 19 CFR 151.70 - Method of sampling by Customs.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Method of sampling by Customs. 151.70 Section 151... THE TREASURY (CONTINUED) EXAMINATION, SAMPLING, AND TESTING OF MERCHANDISE Wool and Hair § 151.70 Method of sampling by Customs. A general sample shall be taken from each sampling unit, unless it is not...

  12. 48 CFR 2052.211-70 - Preparation of technical reports.

    Science.gov (United States)

    2010-10-01

    ....211-70 Preparation of technical reports. As prescribed at 2011.104-70(a), the contracting officer... Reports (JAN 1993) All technical reports required by Section C and all Technical Progress Reports required... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Preparation of technical...

  13. 7 CFR 70.105 - Procedures for appeal gradings.

    Science.gov (United States)

    2010-01-01

    ... conducting the appeal grading. Whether defrosting poultry or rabbit carcasses for other types of appeals will... 7 Agriculture 3 2010-01-01 2010-01-01 false Procedures for appeal gradings. 70.105 Section 70.105... PRODUCTS AND RABBIT PRODUCTS Grading of Poultry Products and Rabbit Products Appeal of A Grading Or...

  14. 48 CFR 352.224-70 - Privacy Act.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Privacy Act. 352.224-70... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses 352.224-70 Privacy Act. As prescribed in 324.103(b)(2), the Contracting Officer shall insert the following clause: Privacy Act (January...

  15. 16 CFR 802.70 - Acquisitions subject to order.

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Acquisitions subject to order. 802.70 Section 802.70 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENTS AND... the Federal Trade Commission or of any Federal court in an action brought by the Federal Trade...

  16. 27 CFR 70.445 - Commerce in explosives.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Commerce in explosives. 70... Cartridges, and Explosives § 70.445 Commerce in explosives. Part 55 of title 27 CFR contains the regulations..., explosives, (b) Permits for users who buy or transport explosives in interstate or foreign commerce, (c...

  17. 38 CFR 70.21 - Where to apply.

    Science.gov (United States)

    2010-07-01

    ... BENEFICIARY TRAVEL UNDER 38 U.S.C. 111 § 70.21 Where to apply. Claimants for beneficiary travel must submit the information required in § 70.20 to the Chief of the Business Office or other designee at the VA medical facility responsible for the medical care or services being provided and for which travel is...

  18. 29 CFR 70.6 - Disclosure of originals.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Disclosure of originals. 70.6 Section 70.6 Labor Office of... of originals. (a) No original record or file in the custody of the Department of Labor, or of any... or the Inspector General. (b) The individual authorizing the release of the original record or file...

  19. Architecture of the E.coli 70S ribosome

    DEFF Research Database (Denmark)

    Burkhardt, N.; Diedrich, G.; Nierhaus, K.H.

    1997-01-01

    The 70S ribosome from E.coli was analysed by neutron scattering focusing on the shape and the internal protein-RNA-distribution of the complex. Measurements on selectively deuterated 70S particles and free 30S and 50S subunits applying conventional contrast variation and proton-spin contrast...

  20. 46 CFR 199.70 - Personal lifesaving appliances.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Personal lifesaving appliances. 199.70 Section 199.70 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LIFESAVING APPLIANCES AND ARRANGEMENTS... appliances. (a) Lifebuoys. Each vessel must carry lifebuoys approved under approval series 160.150 as follows...

  1. 46 CFR 70.28-1 - Lifesaving appliances and arrangements.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Lifesaving appliances and arrangements. 70.28-1 Section... PROVISIONS Lifesaving Appliances and Arrangements § 70.28-1 Lifesaving appliances and arrangements. All lifesaving appliances and arrangements on passenger vessels must be in accordance with subchapter W...

  2. Imaging of Hsp70-positive tumors with cmHsp70.1 antibody-conjugated gold nanoparticles

    Directory of Open Access Journals (Sweden)

    Gehrmann MK

    2015-09-01

    Full Text Available Mathias K Gehrmann,1 Melanie A Kimm,2 Stefan Stangl,1 Thomas E Schmid,1 Peter B Noël,2 Ernst J Rummeny,2 Gabriele Multhoff11Department of Radiation Oncology, 2Department of Diagnostic and Interventional Radiology, Klinikum rechts der Isar, Technische Universität München, Munich, GermanyAbstract: Real-time imaging of small tumors is still one of the challenges in cancer diagnosis, prognosis, and monitoring of clinical outcome. Targeting novel biomarkers that are selectively expressed on a large variety of different tumors but not normal cells has the potential to improve the imaging capacity of existing methods such as computed tomography. Herein, we present a novel technique using cmHsp70.1 monoclonal antibody-conjugated spherical gold nanoparticles for quantification of the targeted uptake of gold nanoparticles into membrane Hsp70-positive tumor cells. Upon binding, cmHsp70.1-conjugated gold nanoparticles but not nanoparticles coupled to an isotype-matched IgG1 antibody or empty nanoparticles are rapidly taken up by highly malignant Hsp70 membrane-positive mouse tumor cells. After 24 hours, the cmHsp70.1-conjugated gold nanoparticles are found to be enriched in the perinuclear region. Specificity for membrane Hsp70 was shown by using an Hsp70 knockout tumor cell system. Toxic side effects of the cmHsp70.1-conjugated nanoparticles are not observed at a concentration of 1–10 µg/mL. Experiments are ongoing to evaluate whether cmHsp70.1 antibody-conjugated gold nanoparticles are suitable for the detection of membrane-Hsp70-positive tumors in vivo.Keywords: heat shock protein 70, tumor biomarker, theranostics, multimodal CT, multispectral CT, k-edge

  3. Inhibition of Ku70 acetylation by INHAT subunit SET/TAF-Iβ regulates Ku70-mediated DNA damage response.

    Science.gov (United States)

    Kim, Kee-Beom; Kim, Dong-Wook; Park, Jin Woo; Jeon, Young-Joo; Kim, Daehwan; Rhee, Sangmyung; Chae, Jung-Il; Seo, Sang-Beom

    2014-07-01

    DNA double-strand breaks (DSBs) can cause either cell death or genomic instability. The Ku heterodimer Ku70/80 is required for the NHEJ (non-homologous end-joining) DNA DSB repair pathway. The INHAT (inhibitor of histone acetyltransferases) complex subunit, SET/TAF-Iβ, can inhibit p300- and PCAF-mediated acetylation of both histone and p53, thereby repressing general transcription and that of p53 target genes. Here, we show that SET/TAF-Iβ interacts with Ku70/80, and that this interaction inhibits CBP- and PCAF-mediated Ku70 acetylation in an INHAT domain-dependent manner. Notably, DNA damage by UV disrupted the interaction between SET/TAF-Iβ and Ku70. Furthermore, we demonstrate that overexpressed SET/TAF-Iβ inhibits recruitment of Ku70/80 to DNA damage sites. We propose that dysregulation of SET/TAF-Iβ expression prevents repair of damaged DNA and also contributes to cellular proliferation. All together, our findings indicate that SET/TAF-Iβ interacts with Ku70/80 in the nucleus and inhibits Ku70 acetylation. Upon DNA damage, SET/TAF-Iβ dissociates from the Ku complex and releases Ku70/Ku80, which are then recruited to DNA DSB sites via the NHEJ DNA repair pathway.

  4. Want independent validation and assurance? Ask for a SAS-70.

    Science.gov (United States)

    Boutin, Christopher C

    2008-08-01

    The AICPA's Statement on Auditing Standards No.70, Service Organizations addresses CPA audits of service providers conducted to verify that a provider has adequate controls over its operations. Hospitals should request a SAS-70, the report produced by such an audit, from all of their third-party service providers. SAS-70s can be issued for a specific date or for a six-month period, and they typically consist of three sections: a CPA opinion, a description of controls, and information about the design of the controls.

  5. 28 CFR 70.42 - Codes of conduct.

    Science.gov (United States)

    2010-07-01

    ...-PROFIT ORGANIZATIONS Post-Award Requirements Procurement Standards § 70.42 Codes of conduct. The..., recipients may set standards for situations in which the financial interest is not substantial or the gift is...

  6. 48 CFR 1515.305-70 - Scoring plans.

    Science.gov (United States)

    2010-10-01

    ... METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Source Selection 1515.305-70 Scoring plans. When... solicitation, e.g., other numeric, adjectival, color rating systems, etc. Scoring Plan Value Descriptive...

  7. Acoustic Multi-Beam Echosounder Data (ME70)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Southeast Fisheries Science Center Mississippi Laboratories collects data using a Simrad ME70 scientific multibeam acoustic echosounder during resource...

  8. VT Predicted Mean Wind Speed - 70 meter height

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) Wind speed predictions at 70m are generated by a numerical model that simulates weather conditions over a 15-year period, taking into account...

  9. 28 CFR 70.28 - Period of availability of funds.

    Science.gov (United States)

    2010-07-01

    ... REQUIREMENTS FOR GRANTS AND AGREEMENTS (INCLUDING SUBAWARDS) WITH INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Financial and Program Management § 70.28...

  10. PROTVINO: U-70's first long run

    International Nuclear Information System (INIS)

    Bougorski, A.

    1995-01-01

    The Protvino 70 GeV proton synchrotron (U-70), commissioned in 1967, was for five years the world's largest proton machine. In all, more than 170 experiments at U-70 have been carried out, more than 50 being joint experiments with physicists from the European, USA and Japan laboratories. Under the Russian government, the Protvino Institute for High Energy Physics' high scientific authority was last year accorded the title of State Scientific Centre, denoting that it is the major laboratory in Russia carrying out a high energy physics programme actually in the country. In keeping with this status, U-70's traditional running schedule has been boosted. Before 1994, U-70 was scheduled for four or five runs, each of about 40 days, each year. Meanwhile a major upgrade of the IHEP machine had been launched. Among its main goals were the replacement of many U-70 systems to increase the proton beam intensity and the machine's operational reliability, to carry out a new physics programme at 70 GeV, and to obtain the beam conditions necessary for the big UNK machine under construction. The U-70 upgrade includes: total replacement of the goffered vacuum chamber by a smooth one; improvement of all 40 radiofrequency stations; design and construction of a new diagnostic and control system; and design and construction of a new 60 MeV radiofrequency linac to allow acceleration of negative hydrogen ions and charge exchange injection. At the same time U-70 was converted for longer running periods, with two longer runs per year with a summer break for maintenance, upgrades and installation. In 1994, despite the adverse financial situation which Russian science and particularly high energy physics is undergoing, the first stage of the U-70 upgrade was completed and, for the first time in IHEP's history, a three-month (October-December) run was scheduled. This run yielded the full harvest of expected data and all accelerator systems operated reliably under the

  11. 27 CFR 70.103 - Failure to pay tax.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Failure to pay tax. 70.103... § 70.103 Failure to pay tax. Whoever fails to pay any tax imposed by Part I of Subchapter A of Chapter... penalty of 5 percent of the tax due but unpaid. For additional penalties for failure to pay tax, see 27...

  12. 27 CFR 70.26 - Third-party recordkeepers.

    Science.gov (United States)

    2010-04-01

    .... 70.26 Section 70.26 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU...) Definitions—(1) Accountant. A person is an “accountant” under 26 U.S.C. 7609(a)(3)(F) for purposes of... certified under State law as an accountant. (2) Attorney. A person is an “attorney” under 26 U.S.C. 7609(a...

  13. Crystal structures of the ATPase domains of four human Hsp70 isoforms: HSPA1L/Hsp70-hom, HSPA2/Hsp70-2, HSPA6/Hsp70B', and HSPA5/BiP/GRP78.

    Science.gov (United States)

    Wisniewska, Magdalena; Karlberg, Tobias; Lehtiö, Lari; Johansson, Ida; Kotenyova, Tetyana; Moche, Martin; Schüler, Herwig

    2010-01-11

    The 70-kDa heat shock proteins (Hsp70) are chaperones with central roles in processes that involve polypeptide remodeling events. Hsp70 proteins consist of two major functional domains: an N-terminal nucleotide binding domain (NBD) with ATPase activity, and a C-terminal substrate binding domain (SBD). We present the first crystal structures of four human Hsp70 isoforms, those of the NBDs of HSPA1L, HSPA2, HSPA5 and HSPA6. As previously with Hsp70 family members, all four proteins crystallized in a closed cleft conformation, although a slight cleft opening through rotation of subdomain IIB was observed for the HSPA5-ADP complex. The structures presented here support the view that the NBDs of human Hsp70 function by conserved mechanisms and contribute little to isoform specificity, which instead is brought about by the SBDs and by accessory proteins. This article can also be viewed as an enhanced version in which the text of the article is integrated with interactive 3D representations and animated transitions. Please note that a web plugin is required to access this enhanced functionality. Instructions for the installation and use of the web plugin are available in Text S1.

  14. Crystal structures of the ATPase domains of four human Hsp70 isoforms: HSPA1L/Hsp70-hom, HSPA2/Hsp70-2, HSPA6/Hsp70B', and HSPA5/BiP/GRP78.

    Directory of Open Access Journals (Sweden)

    Magdalena Wisniewska

    2010-01-01

    Full Text Available The 70-kDa heat shock proteins (Hsp70 are chaperones with central roles in processes that involve polypeptide remodeling events. Hsp70 proteins consist of two major functional domains: an N-terminal nucleotide binding domain (NBD with ATPase activity, and a C-terminal substrate binding domain (SBD. We present the first crystal structures of four human Hsp70 isoforms, those of the NBDs of HSPA1L, HSPA2, HSPA5 and HSPA6. As previously with Hsp70 family members, all four proteins crystallized in a closed cleft conformation, although a slight cleft opening through rotation of subdomain IIB was observed for the HSPA5-ADP complex. The structures presented here support the view that the NBDs of human Hsp70 function by conserved mechanisms and contribute little to isoform specificity, which instead is brought about by the SBDs and by accessory proteins.This article can also be viewed as an enhanced version in which the text of the article is integrated with interactive 3D representations and animated transitions. Please note that a web plugin is required to access this enhanced functionality. Instructions for the installation and use of the web plugin are available in Text S1.

  15. KADAR MDA DAN HSP 70 PADA PLASENTA PENDERITA PREEKLAMPSIA

    Directory of Open Access Journals (Sweden)

    Arleni

    2008-12-01

    Full Text Available The MDA and HSP70 Concentration in Preeclamptic Patient Placenta’s. Objective: Preeclampsia is a disease in pregnancy and characterized by hypertension and proteinuria. Preeclampsia and eclampsia are the most causes of maternal and fetal mortality and morbidity in Indonesia. Placental and systemic oxidative stress caused endothelial cell dysfunction and injury. Placental oxidative stress also linked to fetal growth restriction. HSP70 is essential for cellular recovery, survival and maintenance of homeostasis. The purpose of this study was to compare the MDA, a marker for oxidative stress and HSP70 production in placental of severe preeclampsia, mild preeclampsia and normotensive pregnant women. Placenta were collected after delivery from normotensive pregnancies (N=10, severe preeclampsia (N=10 and mild preeclampsia (N=10. Placenta was cultured in RPMI and 20% FBS, and supernatant were collected in day 3. MDA was measured using spectrophotometer and absorbance read in 530nm. HSP70 was measured using enzyme-linked immunosorbent assay. The mean MDA concentration did not differ significantly between patients with severe preeclampsia (7.13+5.36 nmol/ml and mild preeclampsia (4.82+2.47 nmol/ml when compared with normotensive pregnancies (4.57+2.4 nmol/ml. The mean HSP70 concentration in mild preeclampsia is highest (10.15+12.39 nmo/ml when compared with severe preeclampsia (3.78 +3.07 nmol/ml and normotensive pregnant women (3.76+4.65nmol/ml, but the difference was not significant. Although the difference was not significant, is indicates homeostasis response in mild preclampsia women is relative good. This response was abated in severe preeclampstic women. Although MDA and HSP70 concentration did not differ significantly between groups, however the high HSP70 concentration is indicates homeostasis response relatively good in mild preeclamptic women.

  16. Experimental evidence for rhombohedral phase of C70 after irradiation

    International Nuclear Information System (INIS)

    Misof, K.; Fratzl, P.; Vogl, G.

    1993-01-01

    Purely f.c.c. C 70 was irradiated with high-energy heavy ions at low temperature. This led to the appearance of additional Bragg peaks attributed to an irradiation-induced f.c.c.-to-rhombohedral phase transition. On heating to about 570 K the retrasition to the pure f.c.c. phase occurred. Since no irradiation-induced phase transition was obsered in C 60 , the rhombohedral phase appears to be connected with the ''rugby-ball shape'' of C 70 which favours the alignment of the molecules. We propose that irradiation provides the agitation enabling the C 70 molecules to overcome the small energy barriers which otherwise prevent the alignment of the rugby balls over larger regions. (orig.)

  17. MCSD certification toolkit (exam 70-483) programming in C#

    CERN Document Server

    Covaci, Tiberiu; Varallo, Vincent; O'Brien, Gerry

    2013-01-01

    A perfectly crafted prep guide that prepares you for the MCSD 70-483 The MCSD 70-483 exam is the entry-level Microsoft certification exam for C# developers and this must-have resource offers essential coverage of the exam that will test your competency in C# programming. Each chapter covers one of the core subject domains that comprise the exam. Among the authors are experienced trainers who advised Microsoft on the development of its certification programs, affording them a unique understanding of both the objectives and what it takes to master them. This invaluable knowledge is p

  18. SAGE Version 7.0 Algorithm: Application to SAGE II

    Science.gov (United States)

    Damadeo, R. P; Zawodny, J. M.; Thomason, L. W.; Iyer, N.

    2013-01-01

    This paper details the Stratospheric Aerosol and Gas Experiments (SAGE) version 7.0 algorithm and how it is applied to SAGE II. Changes made between the previous (v6.2) and current (v7.0) versions are described and their impacts on the data products explained for both coincident event comparisons and time-series analysis. Users of the data will notice a general improvement in all of the SAGE II data products, which are now in better agreement with more modern data sets (e.g. SAGE III) and more robust for use with trend studies.

  19. The stress protein heat shock cognate 70 (Hsc70) inhibits the Transient Receptor Potential Vanilloid type 1 (TRPV1) channel.

    Science.gov (United States)

    Iftinca, Mircea; Flynn, Robyn; Basso, Lilian; Melo, Helvira; Aboushousha, Reem; Taylor, Lauren; Altier, Christophe

    2016-01-01

    Specialized cellular defense mechanisms prevent damage from chemical, biological, and physical hazards. The heat shock proteins have been recognized as key chaperones that maintain cell survival against a variety of exogenous and endogenous stress signals including noxious temperature. However, the role of heat shock proteins in nociception remains poorly understood. We carried out an expression analysis of the constitutively expressed 70 kDa heat-shock cognate protein, a member of the stress-induced HSP70 family in lumbar dorsal root ganglia from a mouse model of Complete Freund's Adjuvant-induced chronic inflammatory pain. We used immunolabeling of dorsal root ganglion neurons, behavioral analysis and patch clamp electrophysiology in both dorsal root ganglion neurons and HEK cells transfected with Hsc70 and Transient Receptor Potential Channels to examine their functional interaction in heat shock stress condition. We report an increase in protein levels of Hsc70 in mouse dorsal root ganglia, 3 days post Complete Freund's Adjuvant injection in the hind paw. Immunostaining of Hsc70 was observed in most of the dorsal root ganglion neurons, including the small size nociceptors immunoreactive to the TRPV1 channel. Standard whole-cell patch-clamp technique was used to record Transient Receptor Potential Vanilloid type 1 current after exposure to heat shock. We found that capsaicin-evoked currents are inhibited by heat shock in dorsal root ganglion neurons and transfected HEK cells expressing Hsc70 and TRPV1. Blocking Hsc70 with matrine or spergualin compounds prevented heat shock-induced inhibition of the channel. We also found that, in contrast to TRPV1, both the cold sensor channels TRPA1 and TRPM8 were unresponsive to heat shock stress. Finally, we show that inhibition of TRPV1 depends on the ATPase activity of Hsc70 and involves the rho-associated protein kinase. Our work identified Hsc70 and its ATPase activity as a central cofactor of TRPV1 channel function

  20. 48 CFR 801.602-70 - General review requirements.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false General review... AFFAIRS GENERAL DEPARTMENT OF VETERANS AFFAIRS ACQUISITION REGULATION SYSTEM Career Development, Contracting Authority, and Responsibilities 801.602-70 General review requirements. (a) Contracting officers...

  1. 48 CFR 1816.307-70 - NASA contract clauses.

    Science.gov (United States)

    2010-10-01

    ... officer may insert a clause substantially as stated at 1852.216-87, Submission of Vouchers for Payment, in... ADMINISTRATION CONTRACTING METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Cost-Reimbursement Contracts 1816.307-70... clause at 1852.216-75, Payment of Fixed Fee, in cost-plus-fixed-fee contracts. Modifications to the...

  2. 48 CFR 1339.107-70 - Information security.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Information security. 1339... CATEGORIES OF CONTRACTING ACQUISITION OF INFORMATION TECHNOLOGY General 1339.107-70 Information security. (a... coordinate with the designated Contracting Officer Representative (COR) to complete the Information Security...

  3. 50 CFR 18.70 - Basis and purpose.

    Science.gov (United States)

    2010-10-01

    ... importing of animals from each species of marine mammals under his jurisdiction; and (3) prescribe... PLANTS (CONTINUED) MARINE MAMMALS Notice and Hearing on Section 103 Regulations § 18.70 Basis and purpose. (a) Sections 101(a)(2), 101(a)(3)(A), and 101(b) of the Marine Mammal Protection Act of 1972 (16 U.S...

  4. 48 CFR 352.231-70 - Salary rate limitation.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Salary rate limitation... Salary rate limitation. As prescribed in 331.101-70, the Contracting Officer shall insert the following clause: Salary Rate Limitation (January 2010) (a) Pursuant to the current and applicable prior HHS...

  5. 27 CFR 70.242 - Wages, salary and other income.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Wages, salary and other... Collection of Excise and Special (Occupational) Tax Limitations § 70.242 Wages, salary and other income. (a... as wages, salary or other income are exempt from levy. This section described the income of a...

  6. 48 CFR 331.101-70 - Salary rate limitation.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Salary rate limitation... CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Applicability 331.101-70 Salary rate... used to pay the direct salary of an individual at a rate in excess of the Federal Executive Schedule...

  7. Thermostability of xylanolytic enzymes produced by Lentinula edodes UFV70

    Science.gov (United States)

    Ribeiro, Liliane Fraga Costa; Vaz, Marcelo Gomes Marçal Vieira; Chaves-Alves, Virgínia Maria; Vanetti, Maria Cristina Dantas; Vanetti, Maria Cristina Dantas; Kasuya, Maria Catarina Megumi; Passos, Flávia Maria Lopes; do Nascimento, Antônio Galvão

    2012-01-01

    Xylanolytic enzymes produced by Lentinula edodes UFV70, cultivated in eucalyptus sawdust/rice bran medium, were stable at 50, 60 and 65°C for 21 hours, losing only 15–25% activity. Fungus incubation at 50°C for 12 hours and at 65°C for 24 hours increased the amount of xylose produced. PMID:24031818

  8. 48 CFR 1452.226-70 - Indian Preference.

    Science.gov (United States)

    2010-10-01

    ...); and (3) “Indian-owned economic enterprise” means any Indian-owned commercial, industrial, or business... MANAGEMENT SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 1452.226-70 Indian...-owned economic enterprises in the awarding of any subcontracts consistent with the efficient performance...

  9. 33 CFR 115.70 - Advance approval of bridges.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Advance approval of bridges. 115... BRIDGES BRIDGE LOCATIONS AND CLEARANCES; ADMINISTRATIVE PROCEDURES § 115.70 Advance approval of bridges. (a) The General Bridge Act of 1946 requires the approval of the location and plans of bridges prior...

  10. 48 CFR 515.305-70 - Use of outside evaluators.

    Science.gov (United States)

    2010-10-01

    ... CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Source Selection 515.305-70 Use of outside... evaluators. (2) Each evaluator will sign and provide to GSA a “Conflict of Interest Acknowledgment and Nondisclosure Agreement.” Figure 515.3-1—Conflict of Interest Acknowledgment and Nondisclosure Agreement...

  11. 48 CFR 849.111-70 - Required review.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Required review. 849.111... MANAGEMENT TERMINATION OF CONTRACTS General Principles 849.111-70 Required review. (a) FAR 49.111 requires each agency to establish procedures, when necessary, for the administrative review of proposed...

  12. 27 CFR 70.414 - Preparation and filing of claims.

    Science.gov (United States)

    2010-04-01

    ... Spirits, Wines, and Beer § 70.414 Preparation and filing of claims. (a) Distilled spirits at distilled.... (d) Wine spirits and wine at bonded wine cellar. Procedural instructions in respect of claims for: (1) Remission of tax on wine spirits lost on the premises of a bonded wine cellar or in transit thereto, (2...

  13. thermal stress and Hsp70 as selective agents

    Indian Academy of Sciences (India)

    Madhu Sudhan

    starred in an exhaustive list of studies linking its expression to environmental ... genetic stress linked to Hsp70: polyglutamine expansion .... Twenty-five D. melanogaster genes conserved in all twelve Drosophila species (fourth column) possessing conserved ...... like growth factor-1, and vertebral bone mass in men; J. Clin.

  14. 48 CFR 352.231-70 - Precontract costs.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Precontract costs. 352.231... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 352.231-70 Precontract costs. As prescribed in 48 CFR 1331.205-32, insert the following clause: Precontract Costs (APR 2010) The contractor is...

  15. 27 CFR 70.81 - Notice and demand for tax.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Notice and demand for tax... Excise and Special (Occupational) Tax Notice and Demand § 70.81 Notice and demand for tax. (a) General... demanding payment thereof. Such notice shall be given as soon as possible and within 60 days. However, the...

  16. 28 CFR 79.70 - Attorney General's delegation of authority.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Attorney General's delegation of... RADIATION EXPOSURE COMPENSATION ACT Procedures § 79.70 Attorney General's delegation of authority. (a) An... claim filed under the Act, and otherwise act on behalf of the Attorney General in all other matters...

  17. 48 CFR 235.006-70 - Manufacturing Technology Program.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Manufacturing Technology... CONTRACTING 235.006-70 Manufacturing Technology Program. In accordance with 10 U.S.C. 2521(d), for acquisitions under the Manufacturing Technology Program— (a) Award all contracts using competitive procedures...

  18. 48 CFR 952.242-70 - Technical direction.

    Science.gov (United States)

    2010-10-01

    ... contract. (d) All technical direction shall be issued in writing by the COR. (e) The Contractor must... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Technical direction. 952... FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 952.242-70 Technical...

  19. 10 CFR 70.32 - Conditions of licenses.

    Science.gov (United States)

    2010-01-01

    ... chapter shall furnish to the Director, Division of Security Policy, Office of Nuclear Security and... change to the Director, Division of Security Policy, Office of Nuclear Security and Incident Response... listed in § 70.5(a), furnish the Director, Division of Security Policy, Office of Nuclear Security and...

  20. 27 CFR 70.302 - Fees and costs for witnesses.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Fees and costs for... Collection of Excise and Special (Occupational) Tax Miscellaneous Provisions § 70.302 Fees and costs for... costs. Directly incurred costs are costs incurred solely, immediately, and necessarily as a consequence...

  1. 48 CFR 1815.207-70 - Release of proposal information.

    Science.gov (United States)

    2010-10-01

    ... Announcements of Opportunity and NASA Research Announcements; (ii) Unsolicited proposals; and (iii) SBIR and... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Release of proposal... Receipt of Proposals and Information 1815.207-70 Release of proposal information. (a) NASA personnel...

  2. 48 CFR 229.402-70 - Additional clauses.

    Science.gov (United States)

    2010-10-01

    ... performance will be in Spain. (f) Use the clause at 252.229-7006, Value Added Tax Exclusion (United Kingdom.... (i) Use the clause at 252.229-7009, Relief from Customs Duty and Value Added Tax on Fuel (Passenger... SYSTEM, DEPARTMENT OF DEFENSE GENERAL CONTRACTING REQUIREMENTS TAXES Contract Clauses 229.402-70...

  3. 27 CFR 70.141 - Lien for taxes.

    Science.gov (United States)

    2010-04-01

    ... intangible, belonging to such person. The lien attaches to all property and rights to property belonging to such person at any time during the period of the lien, including any property or rights to property... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Lien for taxes. 70.141...

  4. 27 CFR 70.147 - Priority of interest and expenses.

    Science.gov (United States)

    2010-04-01

    ... interest is not subrogated to the rights of the holder of the State sales tax lien. However, if the holder... the rights of the holder of the sales tax lien, the holder of the lien or security interest will also... Collection of Excise and Special (Occupational) Tax Lien for Taxes § 70.147 Priority of interest and expenses...

  5. Praost Thomas Vaga 70 / Juhan Simonson ; foto: Juhan Simonson

    Index Scriptorium Estoniae

    Simonson, Juhan, 1933-2012

    2008-01-01

    8. märtsil 70 aastaseks saavast üle 20 aasta kirikuõpetajana New Jerseys ja New Yorgis teeninud praost Thomas Vagast. President Toomas Hendrik Ilves annetas iseseisvuspäeva puhul Thomas Vagale kui eestluse hoidjale Ameerika Ühendriikides ja kirikuelu edendajale Valgetähe IV klassi teenetemärgi

  6. 48 CFR 1329.203-70 - DOC Federal tax exemption.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false DOC Federal tax exemption... CONTRACTING REQUIREMENTS TAXES Federal Excise Taxes 1329.203-70 DOC Federal tax exemption. (a) The Office of... enabling DOC and its contractors to purchase spirits (e.g., specially denatured spirits) tax-free for non...

  7. 48 CFR 2515.215-70 - NSF negotiation authorities.

    Science.gov (United States)

    2010-10-01

    ... CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Negotiation Authorities 2515.215-70 NSF negotiation authorities. (a) Authorities. Citation: 42 U.S.C. 1870(c). (b) Application. When an NSF contract... international cooperation or national security.” Contracts or their modifications entered into under this...

  8. 27 CFR 70.608 - Action on claims.

    Science.gov (United States)

    2010-04-01

    ... Section 70.608 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT... appropriate TTB officer shall date stamp and examine each claim filed under this subpart and will determine the validity of the claim. Claims and supporting data involving customs duties will be forwarded to...

  9. 27 CFR 70.65 - Use of commercial banks.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Use of commercial banks... Excise and Special (Occupational) Tax Receipt of Payment § 70.65 Use of commercial banks. For provisions relating to the use of commercial banks and electronic fund transfer of taxpayment to the Treasury Account...

  10. 48 CFR 2803.104-70 - Ethics program training requirements.

    Science.gov (United States)

    2010-10-01

    ... contracting officers and procurement officials. The Ethics Official should be contacted directly to schedule... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Ethics program training... General IMPROPER BUSINESS PRACTICES AND PERSONAL CONFLICTS OF INTEREST Safeguards 2803.104-70 Ethics...

  11. 48 CFR 452.211-70 - Brand Name or Equal.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Brand Name or Equal. 452... FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses 452.211-70 Brand Name or Equal. As prescribed in 411.171, insert the following provision: Brand Name or Equal (NOV 1996...

  12. 28 CFR 70.21 - Standards for financial management systems.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Standards for financial management..., HOSPITALS AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Financial and Program Management § 70.21 Standards for financial management systems. (a) Recipients must relate financial data to...

  13. 48 CFR 614.201-70 - Use of English language.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Use of English language... METHODS AND CONTRACT TYPES SEALED BIDDING Solicitation of Bids 614.201-70 Use of English language. Use of English language solicitations and contracts is mandatory unless a deviation has been approved by the...

  14. 48 CFR 1019.202-70-3 - Non-affiliation.

    Science.gov (United States)

    2010-10-01

    ... SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 1019.202-70-3 Non-affiliation. For purposes of the Small Business Act, a protégé firm may not be considered an affiliate of a mentor firm solely on the basis that... mentor firm under the Program. ...

  15. 48 CFR 3452.209-70 - Organizational conflict of interest.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Organizational conflict of... Clauses 3452.209-70 Organizational conflict of interest. As prescribed in 3409.570, insert the following provision in all certifications: Organizational Conflict of Interest (OCT 1987) The offeror certifies that...

  16. 27 CFR 70.165 - Production of books.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Production of books. 70... books. If a levy has been made or is about to be made on any property or rights to property, any person, having custody or control of any books or records containing evidence or statements relating to the...

  17. 27 CFR 70.22 - Examination of books and witnesses.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Examination of books and... Discovery of Liability and Enforcement of Laws Examination and Inspection § 70.22 Examination of books and... officer may examine any books, papers, records or other data which may be relevant or material to such...

  18. 27 CFR 70.701 - Rules and regulations.

    Science.gov (United States)

    2010-04-01

    ..., Regulations and Forms § 70.701 Rules and regulations. (a) Formulation. (1) Alcohol, tobacco, and firearms... reaching the same conclusion in other cases unless the facts and circumstances are substantially the same...) Disclosure of secret formulas, processes, business practices, and other similar information. (iii)(A) It is...

  19. 48 CFR 352.237-70 - Pro-Children Act.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Pro-Children Act. 352.237...-Children Act. As prescribed in 337.103-70(a), the Contracting Officer shall insert the following clause: Pro-Children Act (January 2006) (a) Public Law 103-227, Title X, Part C, also known as the Pro...

  20. 48 CFR 1809.206-70 - Small businesses.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Small businesses. 1809.206...-70 Small businesses. If a small business otherwise eligible for award has been placed in a special... that the small business does not appear to have the capacity to perform, the certificate of competency...

  1. 48 CFR 2152.210-70 - Investment income.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Investment income. 2152.210... CONTRACT CLAUSES Text of Provisions and Clauses 2152.210-70 Investment income. As prescribed in 2110.7004(a), insert the following clause: Investment Income (OCT 2005) (a) The Contractor must invest and reinvest all...

  2. 14 CFR 298.70 - Public disclosure of data.

    Science.gov (United States)

    2010-01-01

    ... Disclosure of Data § 298.70 Public disclosure of data. (a) Detailed domestic on-flight market data and nonstop segment data except military data shall be made publicly available after processing. Domestic data... operations are reported under service codes N or R. (b) Detailed international on-flight market and nonstop...

  3. 48 CFR 1615.404-70 - Profit analysis factors.

    Science.gov (United States)

    2010-10-01

    ... CONTRACTING BY NEGOTIATION Contract Pricing 1615.404-70 Profit analysis factors. (a) OPM contracting officers... managerial expertise and effort. Evidence of effective contract performance will receive a plus weight, and... indifference to cost control will generally result in a negative weight. (2) Contract cost risk. In assessing...

  4. 99mTc-Dextran-70: preparation and quality control

    International Nuclear Information System (INIS)

    Herrerias, Rosana; Muramoto, Emiko; Hamada, Elena S.; Barboza, Marycel F. de; Silva, Constancia P.G. da

    1997-01-01

    Dextran-70 labelled with 99m Tc is used for lymphocintigraphy in Nuclear Medicine. The aims of this work were: the lyophilized kit formulation; the radiochemical quality control determination and the biodistribution studies in Wistar rats. Each lyophilized vial contains: 50 mg Dextran-70 (Sigma); 750 μg Sn Cl 2 . 2 H 2 O, pH = 4.0. For the radiochemical determination the following parameters were assayed: Chromatography systems (Whatman 3MM, TLC-SG (Silica-gel) e TLC-A1 (aluminium); the 99m Tc activities (37, 111 and 1850 MBq); the 99m Tc volumes (1,3,5 and 8 mL) and the stability after the lyophilization process (1, 3, 6, 12 and 24 months). The Whatman 3MM chromatography system using acetone as solvent presented a purity yield of 99.88; 99.70; 99.00 and 98.92% using 1, 3, 5 and 8 mL of 99m Tc, respectively. The yield of labelling showed 99.80 % of radiochemical purity using 1850 MBq of 99m Tc, after 24 months. The biological studies were performed in Wistar rats, average weight 250g, after intravenous administration of 99m Tc-Dextran-70 (2.96 MBq). A slow blood decrease with high hepatic uptake was mesured. The high kidney uptake observed, during the experiment, was due the experiment, was due the fact that the animals were kept under anaesthesic effect. (author). 4 refs., 2 figs., 4 tabs

  5. 30 CFR 70.220 - Status change reports.

    Science.gov (United States)

    2010-07-01

    ... MANDATORY HEALTH STANDARDS-UNDERGROUND COAL MINES Sampling Procedures § 70.220 Status change reports. (a) If there is a change in operational status that affects the respirable dust sampling requirements of this... produced. (iii) Abandoned—the work of all miners has been terminated and production activity has ceased. (2...

  6. 30 CFR 70.100 - Respirable dust standards.

    Science.gov (United States)

    2010-07-01

    ... mine atmosphere during each shift to which each miner in the active workings of each mine is exposed at... sampling device and in terms of an equivalent concentration determined in accordance with § 70.206 (Approved sampling devices; equivalent concentrations). (b) Each operator shall continuously maintain the...

  7. 48 CFR 2452.251-70 - Contractor employee travel.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Contractor employee travel... 2452.251-70 Contractor employee travel. As prescribed in 2451.7001, insert the following clause in all cost-reimbursement solicitations and contracts involving travel: Contractor Employee Travel (OCT 1999...

  8. 48 CFR 2152.215-70 - Contractor records retention.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Contractor records... PRECONTRACT PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 2152.215-70 Contractor records retention. As prescribed in 2115.071, insert the following clause: Contractor Records Retention (OCT 2005...

  9. 48 CFR 1652.204-70 - Contractor records retention.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Contractor records... of FEHBP Clauses 1652.204-70 Contractor records retention. As prescribed in 1604.705 the following clause will be inserted in all FEHB Program contracts. Contractor Records Retention (JUL 2005...

  10. 48 CFR 852.237-70 - Contractor responsibilities.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contractor....237-70 Contractor responsibilities. As prescribed in 837.110, insert the following clause: Contractor Responsibilities (APR 1984) The contractor shall obtain all necessary licenses and/or permits required to perform...

  11. 48 CFR 1845.502-70 - Contractor-acquired property.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Contractor-acquired... Possession of Contractors 1845.502-70 Contractor-acquired property. All contractor-acquired property must be... contractor-acquired. (2) Submission of DD Form 1419, DOD Industrial Plant Requisition, or equivalent format...

  12. 48 CFR 1699.70 - Cost accounting standards.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Cost accounting standards... EMPLOYEES HEALTH BENEFITS ACQUISITION REGULATION CLAUSES AND FORMS COST ACCOUNTING STANDARDS Cost Accounting Standards 1699.70 Cost accounting standards. With respect to all experience-rated contracts currently...

  13. Decline in oral perception from 20 to 70 years.

    Science.gov (United States)

    Canetta, R

    1977-12-01

    120 adults were given a task to determine whether performance on an oral perception task declines with increasing chronological age. Subjects in their 70's performed significantly worse than those in their 60's and younger. This loss of oral perceptual skills is considered relevant to the remediation and training of older individuals with difficulties in speech articulation.

  14. 48 CFR 815.304-70 - Evaluation factor commitments.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Evaluation factor... AFFAIRS CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Source Selection 815.304-70 Evaluation factor commitments. (a) VA contracting officers shall: (1) Include provisions in negotiated...

  15. 48 CFR 1515.606-70 - Contracting methods.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Contracting methods. 1515... CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Unsolicited Proposals 1515.606-70 Contracting methods. The Department of Housing and Urban Development-Independent Agencies Appropriation Act...

  16. Financial Support for Institutional Research, 1969-70.

    Science.gov (United States)

    Pieper, W. C., Jr.

    The Association for Institutional Research conducted a survey of all institutions of higher education in the U.S. and Canada in order to assess the number, size, and financial support of institutional research offices. Data were requested for the 1969-70 academic year. This report is based on the responses of 1,444 institutions that returned the…

  17. 48 CFR 731.205-70 - Overseas recruitment incentive.

    Science.gov (United States)

    2010-10-01

    ... Organizations 731.205-70 Overseas recruitment incentive. Note: the term employee as used in this section means... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Overseas recruitment... of the U.S. Internal Revenue Code (26 U.S.C. 911), such employee is eligible to receive an overseas...

  18. 48 CFR 1535.007-70 - Contract clauses.

    Science.gov (United States)

    2010-10-01

    ... clause at 1552.235-70, Screening Business Information for Claims of Confidentiality, in contracts when... proprietary or confidential by the business that has the right to the information. (b) The Contracting Officer shall insert the clause at 48 CFR 1552.235-71, Treatment of Confidential Business Information, in...

  19. 48 CFR 852.209-70 - Organizational conflicts of interest.

    Science.gov (United States)

    2010-10-01

    ... Clauses 852.209-70 Organizational conflicts of interest. As prescribed in 809.507-1(b), insert the following provision: Organizational Conflicts of Interest (JAN 2008) (a) It is in the best interest of the Government to avoid situations which might create an organizational conflict of interest or where the offeror...

  20. 48 CFR 307.104-70 - Acquisition strategy.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Acquisition strategy. 307... AND ACQUISITION PLANNING ACQUISITION PLANNING Acquisition Planning 307.104-70 Acquisition strategy... designated by the HHS CIO, DASFMP, the CAO, or the cognizant HCA) shall prepare an acquisition strategy using...

  1. The conformational dynamics of the mitochondrial Hsp70 chaperone.

    Science.gov (United States)

    Mapa, Koyeli; Sikor, Martin; Kudryavtsev, Volodymyr; Waegemann, Karin; Kalinin, Stanislav; Seidel, Claus A M; Neupert, Walter; Lamb, Don C; Mokranjac, Dejana

    2010-04-09

    Heat shock proteins 70 (Hsp70) represent a ubiquitous and conserved family of molecular chaperones involved in a plethora of cellular processes. The dynamics of their ATP hydrolysis-driven and cochaperone-regulated conformational cycle are poorly understood. We used fluorescence spectroscopy to analyze, in real time and at single-molecule resolution, the effects of nucleotides and cochaperones on the conformation of Ssc1, a mitochondrial member of the family. We report that the conformation of its ADP state is unexpectedly heterogeneous, in contrast to a uniform ATP state. Substrates are actively involved in determining the conformation of Ssc1. The J protein Mdj1 does not interact transiently with the chaperone, as generally believed, but rather is released slowly upon ATP hydrolysis. Analysis of the major bacterial Hsp70 revealed important differences between highly homologous members of the family, possibly explaining tuning of Hsp70 chaperones to meet specific functions in different organisms and cellular compartments. 2010 Elsevier Inc. All rights reserved.

  2. Full depth bituminous recycling of I-70, Thomas County, Kansas

    Science.gov (United States)

    2004-01-01

    In 1990, 13 full depth asphalt pavement test sections were built on a portion of I-70 in Thomas County, Kansas. Various combinations of hot mix and cold recycle mixes with different additives were used to build the test sections. Two of the test sect...

  3. 48 CFR 3452.216-70 - Additional cost principles.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Additional cost principles... Clauses 3452.216-70 Additional cost principles. Insert the following clause in solicitations and contracts as prescribed in 3416.307(b): Additional Cost Principles (AUG 1987) (a) Bid and Proposal Costs. Bid...

  4. Antibioticaresistentie van uropathogenen bij vrouwen vanaf 70 jaar.

    NARCIS (Netherlands)

    Donker, G.A.

    2006-01-01

    Inleiding: Urineweginfecties (UWI’s) behoren tot de meest voorkomende infecties bij vrouwen en de prevalentie stijgt substantieel bij toenemende leeftijd, namelijk van beneden de 70 jaar 5 tot 10% tot ongeveer 20% bij 80-plussers. Ook recidiverende UWI’s komen bij post-menopauzale vrouwen meer voor

  5. 48 CFR 970.2303-2-70 - General.

    Science.gov (United States)

    2010-10-01

    ... Technologies, Occupational Safety and Drug-Free Work Place 970.2303-2-70 General. (a) The Department of Energy... inclusion of environmental, safety and health clauses in DOE contracts shall be made by the contracting... health program management personnel. (c)(1) For DOE management and operating contracts and other...

  6. 48 CFR 915.404-4-70-4 - Exceptions.

    Science.gov (United States)

    2010-10-01

    ... unusual pricing situations where the weighted guidelines method has been determined by the DOE negotiating... the pricing situation meets any of the circumstances set forth in this section, other methods for... Section 915.404-4-70-4 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND...

  7. 48 CFR 2152.237-70 - Continuity of services.

    Science.gov (United States)

    2010-10-01

    ...-out services for up to 10 months after this contract expires and (2) negotiate in good faith a plan... contract. The Contractor also must, except if prohibited by applicable law, disclose necessary personnel... PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 2152.237-70 Continuity of services. As...

  8. 28 CFR 70.35 - Supplies and other expendable property.

    Science.gov (United States)

    2010-07-01

    ... aggregate value upon termination or completion of the project or program and the supplies are not needed for... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Supplies and other expendable property... AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Property Standards § 70.35 Supplies and...

  9. 48 CFR 2011.104-70 - NRC Clauses.

    Science.gov (United States)

    2010-10-01

    ... ACQUISITION PLANNING DESCRIBING AGENCY NEEDS Delivery or Performance Schedules-Contract Clauses 2011.104-70...) The contracting officer shall insert the clause at 2052.211-72 Financial Status Report, in applicable cost reimbursement solicitations and contracts when detailed assessment of costs is warranted and a...

  10. 48 CFR 1403.101-70 - Technical evaluators and advisors.

    Science.gov (United States)

    2010-10-01

    ... GENERAL IMPROPER BUSINESS PRACTICES AND PERSONAL CONFLICTS OF INTEREST Safeguards 1403.101-70 Technical... committees, must render impartial, technically sound, and objective assistance and advice. (b) With the... a Conflict of Interest Certificate in a format approved by the HCA. If a potential COI exists, the...

  11. Defense Acquisition Research Journal. Volume 21, Number 3, Issue 70

    Science.gov (United States)

    2014-07-01

    affecting the behavioral change beyond issuing a memorandum. Exhortations are no way to develop a sound business process ( Deming , 1982, pp. 65–70), much...Acquisition University Press. Deming , W. E. (1982). Out of the crisis: Quality, productivity and competitive position. New York, NY: Cambridge University

  12. 48 CFR 352.227-70 - Publications and publicity.

    Science.gov (United States)

    2010-10-01

    ... article the Contractor submits for publication shall be promptly sent to the Contracting Officer's... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Publications and publicity... AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses 352.227-70...

  13. 48 CFR 1019.202-70-13 - Developmental assistance.

    Science.gov (United States)

    2010-10-01

    ... SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 1019.202-70-13 Developmental assistance. The forms of developmental assistance a mentor can provide to a protégé include: (a) Management guidance relating to financial management, organizational management, overall business management/planning, business development...

  14. 7 CFR 1728.70 - Procurement of materials.

    Science.gov (United States)

    2010-01-01

    ... AGRICULTURE ELECTRIC STANDARDS AND SPECIFICATIONS FOR MATERIALS AND CONSTRUCTION § 1728.70 Procurement of... Standards Committees. (2) RUS will also determine, on a case-by-case basis, whether to allow use of an... from the industry is not readily available, or the standard designs are not applicable to the borrower...

  15. 48 CFR 1825.1103-70 - Export control.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Export control. 1825.1103...-70 Export control. (a) Background. (1) NASA contractors and subcontractors are subject to U.S. export... 120 through 130, and the Export Administration Regulations (EAR), 15 CFR parts 730 through 799. The...

  16. Synthesis and structure of alkaline earth and rare earth metal doped C70

    International Nuclear Information System (INIS)

    Takenobu, Taishi; Iwasa, Yoshihiro; Ito, Takayoshi; Mitani, Tadaoki

    2001-01-01

    We have investigated the structure sequence of alkaline earth (A=Ba, Sr) and rare earth metal (R=Eu) doped C 70 binary system. X-ray diffraction measurements revealed that there exist at least four stable phases at x=3, 4, 6, and 9 in A x C 70 and two stable phases at x=3, and 9 in R x C 70 . Among them, structural models are presented for Ba 4 C 70 , Sr 3 C 70 , and Eu 3 C 70 . Ba 4 C 70 takes an analogous structure to orthorhombic Ba 4 C 60 . Sr 3 C 70 and Eu 3 C 70 have monoclinic cell and their diffraction patterns are quite similar to that of Sm 3 C 70 , which involves a unique C 70 -metal-C 70 dimer structure. Preliminary results of Raman spectroscopy and magnetization measurement suggest the highly reduction state for A 9 C 70 and ferromagnetic interaction for Eu x C 70

  17. Role of heat shock protein 70 in innate alloimmunity

    Directory of Open Access Journals (Sweden)

    Walter G. eLand

    2012-01-01

    Full Text Available This article briefly describes our own experience with the proven demonstration of heat shock protein 70 in reperfused renal allografts from brain-deaddonors and reflects about its potential role as a typical damage-associated molecular pattern (DAMP in the setting of innate alloimmunity. In fact, our group was able to demonstrate a dramatic up-regulation of heat shock protein 70 expression after postischemic reperfusion of renal allografts. Of note, up-regulation of this stress protein expression, although to a lesser extent, was already observed after cold storage of the organ indicating that this molecule is already induced in the stressed organism of a brain-dead donor. However, whether or not the dramatic up-regulation of heat shock protein 70 expression contributes to mounting an innate alloimmune response cannot be judged in view of these clinical findings.Nevertheless, heat shock protein 70, since generated in association with postischemic reperfusion-induced allograft injury, can be called a typical DAMP - as can everymolecule be termed a DAMP that is generated in associationwith any stressful tissue injury regardless of its final positive or negative regulatory function within the innate immune response elicited by it.In fact, as we discuss in this article, the context-dependent, even contradistinctive activities of heat shock protein 70 reflect the biological phenomenon that, throughout evolution, mammals have developed an elaborate network of positive and negative regulatory mechanisms, which provide balance between defensive and protective measures against unwarranted destruction of the host. In this sense, up-regulated expression of heat shock protein 70 in an injured allograft might reflect a pure protective response against the severe oxidative injury of a reperfused donor organ. On the other hand, up-regulated expression of this stress protein in an injured allograft might reflect a(futile attempt of the innate immune system to

  18. A Stochastic Slow Extraction Scheme For U70 Synchrotron

    CERN Document Server

    Ivanov, S

    2004-01-01

    Outcomes of a feasibility study for a low-budget sto-chastic slow extraction system in the U70 proton synchro-tron of IHEP are reported. The existing 200 MHz (spill) RF system is to be employed as a longitudinal kicker. It will be driven by a sum of a non-random RF carrier plus an additive random amplitude-modulated signal - either quadrature or in-phase, or both. A few novel solutions to be implemented in the longitudinal diffusion technique that would force protons into the conventional 3-rd order transverse extraction resonance are foreseen so as to com-ply with the technical constraints inherent in U70. Getting a-few-seconds-long and high-quality spills is assessed as being viable with the system in question.

  19. Isomeric rations study for the α + 70 Ge

    International Nuclear Information System (INIS)

    Hora Villano, M.H. da.

    1984-12-01

    Isomeric ratios for 73 Se F,I produced in the reaction α + 70 Ge with incidence laboratory energy ranging from 8 to 28 MeV, have been measured using off-line γ-ray spectroscopy. Relative formation cross-section for isomeric and ground states were obtained with NAT Ge targets. Compound nucleus statistical analyses were performed using computer codes Alice and Julian. Unlike to Alice code, the Julian code predictions agreed quite well with the experimental results. This agreement may be explained by the inclusion of the γ competition in the deexcitation channels of the compound nucleus and by the correct level density calculation of the emission probabilities in the Julian code. Finally angular momentum populations for isomers formations in the reaction 70 Ge(α, n) 73 have been determined. (author)

  20. A 70 MHz pulsing beam system for protons

    International Nuclear Information System (INIS)

    An Shizhong; Zhang Tianjue; Wu Longcheng; Lv Yinlong; Song Guofang; Guan Fengping; Jia Xianlu

    2008-01-01

    A test beam line for pulsed beam generation for 10 MeV central region model (CRM) of a compact cyclotron is under construction as China Institute of Atomic Energy (CIAE). A 70 MHz continuous H - beam with the energy of dozens of keV or a hundred keV will be pulsed to pulse length of less than 10 ns with the repetition rate of 1-8 MHz. A 70.487 MHz buncher will be used to compress the DC beam into the RF phase acceptance of ±30° of the CRM cyclotron. The 2.2 MHz sine waveform will be used for the chopper. A pulse with the repetition rate to 4.4 MHz and pulse length less than 10 ns is expected after CRM cyclotron. (authors)

  1. Men who work at age 70 or older.

    Science.gov (United States)

    Ozawa, Martha N; Lum, Terry Y

    2005-01-01

    The federal policy on older workers has shifted from the encouragement of early withdrawal from the labor force to the encouragement of continuous participation in the labor force. In this light, it is instructive to investigate the backgrounds of elderly people who work at age 70 or older. This article presents the findings of a study, using data from the 1993 Asset and Health Dynamics of the Oldest Old Study, that investigated the effects of health, economic conditions (net worth, employer-provided pensions, and supplemental medical insurance coverage), education, and spouse's work status on the probability of working among men aged 70 or older. The study addressed the probability of working, the probability of working fulltime and of working part-time, and the probability of being self-employed and of being employed by others. Implications for policy are discussed.

  2. Flux pinning in La70 Cu30 disordered system

    International Nuclear Information System (INIS)

    Esquinazi, P.; Guillet, B.; Dussell, H.

    1983-09-01

    Superconducting critical currents were measured as a function of a perpendicular applied magnetic field in disordered La 70 Cu 30 . It is shown that the pinning force is very sensitive to inhomogeneties already present in the as quenched samples, although these inhomogeneties have no obvious influence on other superconducting and transport properties. A simple series resistance model is used to explain the magnetic field dependence of the pinning force. A large decrease of the pinning force was obtained for the annealed samples. This behaviour is consistent with the phase separation model that explains the evolution of the superconductive penetration depth. In order to obtain a better characterization of the structural evolution with annealing of the La 70 Cu 30 system, behaviour of the electrical resistivity, superconducting critical temperature and differential thermal analysis were measured. (author)

  3. Alternate economical starchy substrates for the production of 70% sorbitol

    Energy Technology Data Exchange (ETDEWEB)

    Upadhyay, C.M. (Sarabhai Research Centre, Baroda (India). Industrial Enzymes Dept.); Nehete, P.N. (Sarabhai Research Centre, Baroda (India). Industrial Fermentation Div.); Shah, D.N. (GSFC Research and Development Centre, Fertilizernagar (India). Biotechnology Dept.); Shah, N.K. (Armour Chemicals Pvt. Ltd., Ankleshwar (India)); Shankar, V. (National Chemical Lab., Pune (India). Biochemistry Div.); Kothari, R.M. (Thapar Corporate Research and Development Centre, Patiala (India). Biotechnology Div.)

    1991-03-01

    In view of the soaring prices of corn and tapioca starch, use of their hydrolysate in the production of 70% sorbitol became less remunerative. Therefore, an economical alternative is explored by using hydrolysates of cereal flours, namely, rice (Oryzae sativa), wheat (Triticum aestivum), jowar (Sorghum vulgare) and bajra (Pennisetum typhoideum). A protocol is devised to (a) prepare their high DE hydrolysates, (b) purify it after saccharification, (c) monitor the chemical chracteristics of concentrated hydrolysate, as feedstock for Raney nickel catalyzed pressure hydrogenation and (d) finally prepare 70% sorbital. Merits and demerits of hydrolysates of these cereal flours are discussed in terms of operational limitations and percentage recovery, the governing factors for their industrial acceptability. Rice flour hydrolysate appears to be an alternative substrate, operationally and economically. (orig.).

  4. Order-disorder transitions in C60 and C70

    International Nuclear Information System (INIS)

    Ramasesha, S.K.

    1995-01-01

    In recent years enormous effort has been put in understanding the chemical and physical properties of C 60 and C 70 . Order-disorder transition in C 60 occurs around 250 K at ambient pressure. At the transition freely rotating molecules get orientationally ordered in a simple cubic lattice. Application of pressure increases the transition temperature at a rate of ≅ 10 K kbar -1 , indicating that pressure favours the ordered state. The DSC and x-ray studies on C 70 indicate two phase transitions, one around 270 K and the other around 340 K at room pressure. These transitions also occur at higher temperatures at higher pressures. Application of pressure is found to lift the degeneracy of the energetically equivalent rotational configurations. The high pressure studies are reviewed in the light of existing literature. (author)

  5. 70 years of the general theory of relativity

    International Nuclear Information System (INIS)

    Castro Diaz-Balart, F.; Cabezas Solorzano, R.

    1986-06-01

    In view of the 70th anniversary of the discovery of the General Theory of Relativity, an analysis was made of the special and general theories. The basic postulates, their consequences in the formulation of the theories, the main results, some aspects related to the experimental verification and its applications are presented, as are some elements of the mathematical formalism of the theories, to facilitate the logical interrelationships between its results and consequences. (author)

  6. 70 MeV injector auto tuning system handbook

    International Nuclear Information System (INIS)

    Ellis, J.E.; Munn, R.W.; Sandels, E.G.

    1976-06-01

    The handbook is in three sections: (1) description and location; (2) operating instructions; and (3) design notes on the tank and debuncher auto tuning systems for the 70 MeV injector. The purpose of the auto tuning system is to maintain the 'tune' of the four tanks and debuncher to within a few Hz, stabilizing against changes of temperature and other physical factors affecting the resonant frequency of the tanks. (U.K.)

  7. Fatigue behaviour of X70 steel in crude oil

    Czech Academy of Sciences Publication Activity Database

    Gajdoš, Lubomír; Šperl, Martin; Bystrianský, J.

    2015-01-01

    Roč. 49, č. 2 (2015), s. 243-246 ISSN 1580-2949 R&D Projects: GA ČR(CZ) GAP105/10/2052; GA TA ČR(CZ) TE02000162 Institutional support: RVO:68378297 Keywords : corrosion fatigue * S-N curve * X70 steel * crude oil * separated water Subject RIV: JL - Materials Fatigue, Friction Mechanics Impact factor: 0.439, year: 2015 http://mit.imt.si/Revija/izvodi/mit152/gajdos.pdf

  8. Plasmodium falciparum Hsp70-z, an Hsp110 homologue, exhibits independent chaperone activity and interacts with Hsp70-1 in a nucleotide-dependent fashion.

    Science.gov (United States)

    Zininga, Tawanda; Achilonu, Ikechukwu; Hoppe, Heinrich; Prinsloo, Earl; Dirr, Heini W; Shonhai, Addmore

    2016-05-01

    The role of molecular chaperones, among them heat shock proteins (Hsps), in the development of malaria parasites has been well documented. Hsp70s are molecular chaperones that facilitate protein folding. Hsp70 proteins are composed of an N-terminal nucleotide binding domain (NBD), which confers them with ATPase activity and a C-terminal substrate binding domain (SBD). In the ADP-bound state, Hsp70 possesses high affinity for substrate and releases the folded substrate when it is bound to ATP. The two domains are connected by a conserved linker segment. Hsp110 proteins possess an extended lid segment, a feature that distinguishes them from canonical Hsp70s. Plasmodium falciparum Hsp70-z (PfHsp70-z) is a member of the Hsp110 family of Hsp70-like proteins. PfHsp70-z is essential for survival of malaria parasites and is thought to play an important role as a molecular chaperone and nucleotide exchange factor of its cytosolic canonical Hsp70 counterpart, PfHsp70-1. Unlike PfHsp70-1 whose functions are fairly well established, the structure-function features of PfHsp70-z remain to be fully elucidated. In the current study, we established that PfHsp70-z possesses independent chaperone activity. In fact, PfHsp70-z appears to be marginally more effective in suppressing protein aggregation than its cytosol-localized partner, PfHsp70-1. Furthermore, based on coimmunoaffinity chromatography and surface plasmon resonance analyses, PfHsp70-z associated with PfHsp70-1 in a nucleotide-dependent fashion. Our findings suggest that besides serving as a molecular chaperone, PfHsp70-z could facilitate the nucleotide exchange function of PfHsp70-1. These dual functions explain why it is essential for parasite survival.

  9. $\\beta$-decay half-life of $^{70}$Kr a bridge nuclide for the rp-process beyond A = 70

    CERN Document Server

    Oinonen, M; Jokinen, A; Baumann, P; Didierjean, François; Huck, A; Knipper, A; Ramdhane, M; Walter, G; Van Duppen, P; Huyse, M; Marguier, G; Novikov, Yu N; Popov, A; Seliverstov, D M; Schatz, H

    2000-01-01

    The $\\beta$-decay half-life of $^{70}$Kr has been measured for the first time at the ISOLDE PSB Facility at CERN. Mass separated $^{70}$Kr ions were produced by 1 GeV proton induced spallation reactions in a Nb foil. The measured half-life is 57(21) ms. This value is consistent with the half-life calculated assuming a pure Fermi decay, but is clearly lower than the value used in a recent rp-process reaction flow calculation. The result shows that the reaction flow via two-proton-capture of $^{68}$Se is 2.5 times faster than previously calculated assuming an astrophysical temperature of 1.5 GK and a density of 10$^{6}$g/cm$^{3}$.

  10. Isomer separation of $^{70g}Cu$ and $^{70m}Cu$ with a resonance ionization laser ion source

    CERN Document Server

    Köster, U; Mishin, V I; Weissman, L; Huyse, M; Kruglov, K; Müller, W F; Van Duppen, P; Van Roosbroeck, J; Thirolf, P G; Thomas, H C; Weisshaar, D W; Schulze, W; Borcea, R; La Commara, M; Schatz, H; Schmidt, K; Röttger, S; Huber, G; Sebastian, V; Kratz, K L; Catherall, R; Georg, U; Lettry, Jacques; Oinonen, M; Ravn, H L; Simon, H

    2000-01-01

    Radioactive copper isotopes were ionized with the resonance ionization laser ion source at the on-line isotope separator ISOLDE (CERN). Using the different hyperfine structure in the 3d/sup 10/ 4s /sup 2/S/sub 1/2/-3d/sup 10/ 4p /sup 2/P/sub 1/2//sup 0/ transition the low- and high-spin isomers of /sup 70/Cu were selectively enhanced by tuning the laser wavelength. The light was provided by a narrow-bandwidth dye laser pumped by copper vapor lasers and frequency doubled in a BBO crystal. The ground state to isomeric state intensity ratio could be varied by a factor of 30, allowing to assign gamma transitions unambiguously to the decay of the individual isomers. It is shown that the method can also be used to determine magnetic moments. In a first experiment for the 1/sup +/ ground state of /sup 70/Cu a magnetic moment of (+)1.8(3) mu /sub N/ and for the high-spin isomer of /sup 70/Cu a magnetic moment of (+or-)1.2(3) mu /sub N/ could be deduced. (20 refs).

  11. 40 CFR 427.70 - Applicability; description of the asbestos floor tile subcategory.

    Science.gov (United States)

    2010-07-01

    ... asbestos floor tile subcategory. 427.70 Section 427.70 Protection of Environment ENVIRONMENTAL PROTECTION... Asbestos Floor Tile Subcategory § 427.70 Applicability; description of the asbestos floor tile subcategory... manufacture of asbestos floor tile. ...

  12. Extracts Obtained from Pterocarpus angolensis DC and Ziziphus mucronata Exhibit Antiplasmodial Activity and Inhibit Heat Shock Protein 70 (Hsp70 Function

    Directory of Open Access Journals (Sweden)

    Tawanda Zininga

    2017-07-01

    Full Text Available Malaria parasites are increasingly becoming resistant to currently used antimalarial therapies, therefore there is an urgent need to expand the arsenal of alternative antimalarial drugs. In addition, it is also important to identify novel antimalarial drug targets. In the current study, extracts of two plants, Pterocarpus angolensis and Ziziphus mucronata were obtained and their antimalarial functions were investigated. Furthermore, we explored the capability of the extracts to inhibit Plasmodium falciparum heat shock protein 70 (Hsp70 function. Heat shock protein 70 (Hsp70 are molecular chaperones whose function is to facilitate protein folding. Plasmodium falciparum the main agent of malaria, expresses two cytosol-localized Hsp70s: PfHsp70-1 and PfHsp70-z. The PfHsp70-z has been reported to be essential for parasite survival, while inhibition of PfHsp70-1 function leads to parasite death. Hence both PfHsp70-1 and PfHsp70-z are potential antimalarial drug targets. Extracts of P. angolensis and Z. mucronata inhibited the basal ATPase and chaperone functions of the two parasite Hsp70s. Furthermore, fractions of P. angolensis and Z. mucronata inhibited P. falciparum 3D7 parasite growth in vitro. The extracts obtained in the current study exhibited antiplasmodial activity as they killed P. falciparum parasites maintained in vitro. In addition, the findings further suggest that some of the compounds in P. angolensis and Z. mucronata may target parasite Hsp70 function.

  13. Zinc absorption study using an enriched stable isotope (70Zn)

    International Nuclear Information System (INIS)

    Li Gongpan.

    1990-04-01

    A weaning food from fermented soybean was prepared for increasing the bioavailability of zinc. The zinc absorption was compared with that of a weaning food from non-fermented soybean and normal staple food. A stable isotope tracer technique ( 70 Zn) and neutron activation were used for determining the absorption of zinc. Nine children aged 7 to 18 months were tested. Zinc bioavailability of weaning food from fermented soybean is higher than that of normal weaning food. The weight increment and zinc nutrition of children having weaning food from fermented soybean are improved by this diet. 5 tabs

  14. Constantin Gaindric (in honour of his 70th birthday

    Directory of Open Access Journals (Sweden)

    Editorial board of CSJM

    2011-10-01

    Full Text Available On September 11, 2011, our colleague, Editor-in-Chief of Computer Science Journal of Moldova, Corresponding member Constantin Gaindric, had celebrated his 70-th anniversary. Born in Zaicani, Riscani region, during the Second World War, he was able to withstand adversities of the time, successfully graduating school at Zaicani, followed by the Faculty of Mathematics and Physics of Pedagogical Institute in Balti, after which for almost 50 years he carries out an extraordinary teaching activities, the scientific and managerial ones.

  15. Summary of the 70 GeV Booster Group

    International Nuclear Information System (INIS)

    Makdisi, Y.; Khiari, F.

    1985-06-01

    The energy range of the 70 GeV SSC booster makes it difficult to employ a single technique for preserving the beam polarization. Results of DEPOL calculations show that the expected resonance strengths are below the .5 x 10 -1 level, which poses no problem for resonance jumping. It was found that a single adiabatically energized Siberian snake will not significantly depolarize the beam. Thus one good solution to the mixing problem is that the snake magnets be energized during the acceleration cycle reaching maximum operating value at 20 GeV, where they take over the resonance jumping role. The possibility of adiabatically energizing two snakes was found to be feasible

  16. Genetic and environmental effects of mortality before age 70 years

    DEFF Research Database (Denmark)

    Petersen, Liselotte; Andersen, Per Kragh; Sørensen, Thorkild I.A.

    2008-01-01

    BACKGROUND:: There is a familial influence on risk of many diseases and on mortality in general, which, according to studies of twins, is due to a combination of genetic and environmental effects. Adoption studies, which rest on different assumptions, may also be used to estimate separately...... the genetic and environmental effects on rate of dying. METHODS:: The genetic influence on the rate of dying before age 70 years was investigated by estimation of the associations in total and cause-specific mortality of Danish adoptees and their biologic full and half siblings. Familial environmental...

  17. The National Health Service (NHS) at 70: some comparative reflections.

    Science.gov (United States)

    Tuohy, Carolyn H

    2018-03-16

    As the National Health Service (NHS) turns 70, it bears comparison with another universal system celebrating an anniversary this year: Canada's 50-year-old medicare model. Each system is iconically popular, and each revolves around a profession-state accommodation. Both the popularity and the central axis of each system have been tested by external shocks in the form of periodic fiscal cycles of investment and austerity, and internal stresses generating organizational cycles of centralization and decentralization. In addition, the English NHS has undergone periodic bursts of major policy change, which have arguably moved the system closer to the Canadian single-payer model.

  18. New ENDF/B-7.0 library

    International Nuclear Information System (INIS)

    Oblozinsky, P.

    2008-01-01

    We describe the new version of the Evaluated Nuclear Data File, Endf/B-7.0, of recommended nuclear data for advanced nuclear science and technology applications. The library, produced by the US Cross Section Evaluation Working Group, was released in December 2006. The library contains data in 14 sub-libraries, primarily for reactions with incident neutrons, protons and photons, based on the experimental data and nuclear reaction theory predictions. The neutron reaction sub-library contains data for 393 materials. The new library was extensively tested and shows considerable improvements over the earlier Endf/B-6.8 library. (author)

  19. New models in VERONA 7.0 system

    Energy Technology Data Exchange (ETDEWEB)

    Pos, Istvan; Kalya, Zoltan; Parko, Tamas [Paks Nuclear Power Plant Ltd, Paks (Hungary); Patai-Szabo, Sandor [TS Enercon Ltd., Budapest (Hungary)

    2016-09-15

    Nowadays the installation of a new modernized VERONA core monitoring system (version V7.0) is in process at the NPP Paks. The most important steps of the current improvements are as follows: complete replacement of the hardware and the local area network; application of a new operating system and ''virtual machine'' (VM) technology; implementation of a new human-system interface; and last but not least, introduction of improved reactor physics calculations. Basic novelty of the modernized core analysis is the application of general purpose graphical processing units (GPGPU) in the on-line core-follow module. This new technology has allowed of performing the real-time node-wise core analysis by standard Paks NPP core design codes HELIOS/C-PORCA. The present paper gives a brief overview of the system version (V7.0), focusing to the models of reactor physics and results of validation. Main characteristics of new approaches of the modified on-line reactor physics calculations are also described.

  20. Topical use of dexpanthenol: a 70th anniversary article.

    Science.gov (United States)

    Proksch, Ehrhardt; de Bony, Raymond; Trapp, Sonja; Boudon, Stéphanie

    2017-12-01

    Approximately 70 years ago, the first topical dexpanthenol-containing formulation (Bepanthen™ Ointment) has been developed. Nowadays, various topical dexpanthenol preparations exist, tailored according to individual requirements. Topical dexpanthenol has emerged as frequently used formulation in the field of dermatology and skin care. Various studies confirmed dexpanthenol's moisturizing and skin barrier enhancing potential. It prevents skin irritation, stimulates skin regeneration and promotes wound healing. Two main directions in the use of topical dexpanthenol-containing formulations have therefore been pursued: as skin moisturizer/skin barrier restorer and as facilitator of wound healing. This 70th anniversary paper reviews studies with topical dexpanthenol in skin conditions where it is most frequently used. Although discovered decades ago, the exact mechanisms of action of dexpanthenol have not been fully elucidated yet. With the adoption of new technologies, new light has been shed on dexpanthenol's mode of action at the molecular level. It appears that dexpanthenol increases the mobility of stratum corneum molecular components which are important for barrier function and modulates the expression of genes important for wound healing. This review will update readers on recent advances in this field.

  1. A DOUBLE KNOCKOUT; A NOVEL APPROACH TO UNDERSTANDING STRESS-INDUCIBLE 70 KDA HEAT SHOCK PROTEINS (HSP70S) ON DEVELOPMENT AND REPRODUCTION

    Science.gov (United States)

    Heat and chemical toxicants which disrupt spermatogenesis and cause male infertility are thought to induce the expression of Hsp70-1 and 70-3, the major inducible heat shock proteins of the 70kDa family. Previous studies from several laboratories including our own have characteri...

  2. Correlation between structural relaxation enthalpy and superconducting properties of amorphous Zr70Cu30 and Zr70Ni30 alloys

    International Nuclear Information System (INIS)

    Inoue, A.; Matsuzaki, K.; Toyota, N.; Chen, H.S.; Masumoto, T.; Fukase, T.

    1985-01-01

    The anneal-induced change in the superconducting properties together with the irrecoverable relaxation enthalpy (ΔHsub(i,exo)) and recoverable relaxation enthalpy (ΔHsub(r,endo)) of amorphous Zr 70 Cu 30 and Zr 70 Ni 30 alloys was examined. The increase in ΔHsub(i,exo) and the degradation of Tsub(c) progress logarithmically with annealing time tsub(a) in a temperature range of 373 to 523 K. The activation energy and the attempted frequency were respectively estimated to be 1.5 eV and 6.6 x 10 13 sec -1 for the increase in ΔHsub(i,exo) and 1.5 eV and 1.9 x 10 14 sec -1 for the degradation of Tsub(c). The recoverable structure relaxation exerts little effect on Tsub(c). Based on the agreement between the kinetic parameters for the changes of ΔHsub(i,exo) and Tsub(c), it appears that the degradation of Tsub(c) on annealing is associated with the irrecoverable structural relaxation as a result of the annihilation of frozen-in defects and the topological and compositional atomic rearrangement. The values of the attempted frequency being of the order of Debye frequency suggest that the irrecoverable structural relaxation processes occur more or less independently from each other. (author)

  3. Circulating antibodies to inducible heat shock protein 70 in patients with uveitis

    NARCIS (Netherlands)

    de Smet, M. D.; Ramadan, A.

    2001-01-01

    Heat shock proteins with molecular weight 70 kDa (hsp70) are highly conserved immunogenic intracellular molecules. There are two main subtypes: one is expressed constitutively (hsc70), while the other is induced under stressful conditions (ihsp70). Using an ELISA directed against recombinant human

  4. 48 CFR 1852.244-70 - Geographic participation in the aerospace program.

    Science.gov (United States)

    2010-10-01

    ... the aerospace program. 1852.244-70 Section 1852.244-70 Federal Acquisition Regulations System NATIONAL... Provisions and Clauses 1852.244-70 Geographic participation in the aerospace program. As prescribed in 1844.204-70, insert the following clause: Geographic Participation in the Aerospace Program (APR 1985) (a...

  5. 70 DA WHITE DWARFS IDENTIFIED IN LAMOST PILOT SURVEY

    International Nuclear Information System (INIS)

    Zhao, J. K.; Luo, A. L.; Zhao, G.; Oswalt, T. D.

    2013-01-01

    We present a spectroscopically identified catalog of 70 DA white dwarfs (WDs) from the LAMOST pilot survey. Thirty-five are found to be new identifications after cross-correlation with the Eisenstein et al. and Villanova catalogs. The effective temperature and gravity of these WDs are estimated by Balmer lines fitting. Most of them are hot WDs. The cooling times and masses of these WDs are estimated by interpolation in theoretical evolution tracks. The peak of the mass distribution is found to be ∼0.6 M ☉ , which is consistent with prior work in the literature. The distances of these WDs are estimated using the method of synthetic spectral distances. All of these WDs are found to be in the Galactic disk from our analysis of space motions. Our sample supports the expectation that WDs with high mass are concentrated near the plane of the Galactic disk.

  6. The Complete Structure of the Mycobacterium smegmatis 70S Ribosome

    Directory of Open Access Journals (Sweden)

    Jendrik Hentschel

    2017-07-01

    Full Text Available The ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death worldwide, due in large part to the combination of difficult-to-treat latency and antibiotic resistance. Here, we present the 3.3-Å cryo-EM structure of the 70S ribosome of Mycobacterium smegmatis, a close relative to the human pathogen Mycobacterium tuberculosis. The structure reveals two additional ribosomal proteins and localizes them to the vicinity of drug-target sites in both the catalytic center and the decoding site of the ribosome. Furthermore, we visualized actinobacterium-specific rRNA and protein expansions that extensively remodel the ribosomal surface with implications for polysome organization. Our results provide a foundation for understanding the idiosyncrasies of mycobacterial translation and reveal atomic details of the structure that will facilitate the design of anti-tubercular therapeutics.

  7. Precipitation behaviors of X70 acicular ferrite pipeline steel

    Institute of Scientific and Technical Information of China (English)

    Hao Yu; Yi Sun; Qixiang Chen; Haitao Jiang; Lihong Zhang

    2006-01-01

    The morphology, structure, and chemical composition of precipitates in the final microstructure of Nb-V-Ti microalloyed X70 acicular ferrite pipeline steel were investigated using transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDS). Precipitates observed by TEM can be classified into two groups. The large precipitates are complex compounds that comprise square-shaped TiN precipitate as core with fine Nb-containing precipitate nucleated on pre-existing TiN precipitate as caps on one or more faces at high temperature. In contrast, the fine and spherical Nb carbides and/or carbonitrides precipitate heterogeneously on dislocations and sub-boundaries at low temperature. From the analysis in terms of thermodynamics, EDS and chemical composition of the steel, NbC precipitation is considered to be the predominant precipitation behavior in the tested steel under the processing conditions of this research.

  8. 70 DA WHITE DWARFS IDENTIFIED IN LAMOST PILOT SURVEY

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, J. K.; Luo, A. L.; Zhao, G. [Key Laboratory of Optical Astronomy, National Astronomical Observatories, Chinese Academy of Sciences, Beijing 100012 (China); Oswalt, T. D., E-mail: zjk@bao.ac.cn, E-mail: gzhao@bao.ac.cn, E-mail: lal@bao.ac.cn, E-mail: toswalt@fit.edu [Physics and Space Science Department, Florida Institute of Technology, Melbourne, FL 32901 (United States)

    2013-06-01

    We present a spectroscopically identified catalog of 70 DA white dwarfs (WDs) from the LAMOST pilot survey. Thirty-five are found to be new identifications after cross-correlation with the Eisenstein et al. and Villanova catalogs. The effective temperature and gravity of these WDs are estimated by Balmer lines fitting. Most of them are hot WDs. The cooling times and masses of these WDs are estimated by interpolation in theoretical evolution tracks. The peak of the mass distribution is found to be {approx}0.6 M {sub Sun }, which is consistent with prior work in the literature. The distances of these WDs are estimated using the method of synthetic spectral distances. All of these WDs are found to be in the Galactic disk from our analysis of space motions. Our sample supports the expectation that WDs with high mass are concentrated near the plane of the Galactic disk.

  9. 70 Years of Aeropropulsion Research at NASA Glenn Research Center

    Science.gov (United States)

    Reddy, Dhanireddy R.

    2013-01-01

    This paper presents a brief overview of air-breathing propulsion research conducted at the NASA Glenn Research Center (GRC) over the past 70 years. It includes a historical perspective of the center and its various stages of propulsion research in response to the countrys different periods of crises and growth opportunities. GRCs research and technology development covered a broad spectrum, from a short-term focus on improving the energy efficiency of aircraft engines to advancing the frontier technologies of high-speed aviation in the supersonic and hypersonic speed regimes. This paper highlights major research programs, showing their impact on industry and aircraft propulsion, and briefly discusses current research programs and future aeropropulsion technology trends in related areas

  10. New Directions for MPC and A at Chelyabinsk-70

    International Nuclear Information System (INIS)

    Tsygankov, G.; Churikov, Y.; Bukin, D.; Karpov, A.; Zuev, V.; Blasy, J.; Labiak, B.; Hernandez, J.; Apt, K.; Schultz, F.; Neymotin, L.; Ystesund, K.; Griggs, J.; Cahalane, P.

    2000-01-01

    This paper describes the new directions for the Nuclear Materials Protection, Control, and Accounting (MPC and A) program at the All Russian Scientific Research Institute of Technical Physics (VNIITF), also called Chelyabinsk-70. Chelyabinsk-70 is located in the Ural Mountains, approximately 2000 km east of Moscow and 100 km south of Ekaterinburg. US sponsored MPC and A work has been underway at VNIITF since mid 1995. During the first three years of the VNIITF project, emphasis was on the Pulse Research Reactor Facility (PRR), which contains one metal and two liquid pulse reactors and associated nuclear material storage rooms and a control center. A commissioning of the PRR was held in May of 1998. With the completion of the MPC and A work in the PRR, new physical protection work has focused on building 726, which contains a pulse reactor and a criticality facility. Physical protection work is now complete at building 726. Several changes in the direction of MPC and A work at VNIITF have taken place and others are underway as a result of new DOE Guidelines for MPC and A at Russian Facilities, the National Research Council report issued in late 1999 and other recommendations. A major change is to do MPC and A work only at facilities for which the US can assure the proper categorization of nuclear materials, that upgrades are appropriate, properly installed and operational and that the equipment and funds used to implement and support those upgrades are being utilized in the manner intended. Other changes in direction which will be described include, an increased emphasis on completing inventories, the use of ''inherently sustainable'' upgrades wherever possible, and completing improved accounting systems and other MPC and A upgrades on a prioritized facility by facility basis rather than attempting to implement them site wide

  11. Members of the heat-shock protein 70 family promote cancer cell growth by distinct mechanisms

    DEFF Research Database (Denmark)

    Rohde, Mikkel; Daugaard, Mads; Jensen, Mette Hartvig

    2005-01-01

    Whereas the stress-inducible heat-shock protein 70 (Hsp70) has gained plenty of attention as a putative target for tumor therapy, little is known about the role of other Hsp70 proteins in cancer. Here we present the first thorough analysis of the expression and function of the cytosolic Hsp70...... proteins in human cancer cells and identify Hsp70-2, a protein essential for spermatogenesis, as an important regulator of cancer cell growth. Targeted knock-down of the individual family members by RNA interference revealed that both Hsp70 and Hsp70-2 were required for cancer cell growth, whereas...

  12. 77 FR 50121 - Hospira, Inc.; Withdrawal of Approval of a New Drug Application for DEXTRAN 70

    Science.gov (United States)

    2012-08-20

    ...] Hospira, Inc.; Withdrawal of Approval of a New Drug Application for DEXTRAN 70 AGENCY: Food and Drug... new drug application (NDA) for DEXTRAN 70 (6% Dextran 70 and 0.9% NaCl or/5% Dextrose 500 mL Glass... that FDA withdraw approval of NDA 080-819, DEXTRAN 70 (6% Dextran 70 and 0.9% NaCl or/5% Dextrose 500 m...

  13. Genome-Wide Characterization of Heat-Shock Protein 70s from Chenopodium quinoa and Expression Analyses of Cqhsp70s in Response to Drought Stress.

    Science.gov (United States)

    Liu, Jianxia; Wang, Runmei; Liu, Wenying; Zhang, Hongli; Guo, Yaodong; Wen, Riyu

    2018-01-23

    Heat-shock proteins (HSPs) are ubiquitous proteins with important roles in response to biotic and abiotic stress. The 70-kDa heat-shock genes ( Hsp70s ) encode a group of conserved chaperone proteins that play central roles in cellular networks of molecular chaperones and folding catalysts across all the studied organisms including bacteria, plants and animals. Several Hsp70s involved in drought tolerance have been well characterized in various plants, whereas no research on Chenopodium quinoa HSPs has been completed. Here, we analyzed the genome of C. quinoa and identified sixteen Hsp70 members in quinoa genome. Phylogenetic analysis revealed the independent origination of those Hsp70 members, with eight paralogous pairs comprising the Hsp70 family in quinoa. While the gene structure and motif analysis showed high conservation of those paralogous pairs, the synteny analysis of those paralogous pairs provided evidence for expansion coming from the polyploidy event. With several subcellular localization signals detected in CqHSP70 protein paralogous pairs, some of the paralogous proteins lost the localization information, indicating the diversity of both subcellular localizations and potential functionalities of those HSP70s. Further gene expression analyses revealed by quantitative polymerase chain reaction (qPCR) analysis illustrated the significant variations of Cqhsp70s in response to drought stress. In conclusion, the sixteen Cqhsp70 s undergo lineage-specific expansions and might play important and varied roles in response to drought stress.

  14. The Complete Structure of the Mycobacterium smegmatis 70S Ribosome.

    Science.gov (United States)

    Hentschel, Jendrik; Burnside, Chloe; Mignot, Ingrid; Leibundgut, Marc; Boehringer, Daniel; Ban, Nenad

    2017-07-05

    The ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death worldwide, due in large part to the combination of difficult-to-treat latency and antibiotic resistance. Here, we present the 3.3-Å cryo-EM structure of the 70S ribosome of Mycobacterium smegmatis, a close relative to the human pathogen Mycobacterium tuberculosis. The structure reveals two additional ribosomal proteins and localizes them to the vicinity of drug-target sites in both the catalytic center and the decoding site of the ribosome. Furthermore, we visualized actinobacterium-specific rRNA and protein expansions that extensively remodel the ribosomal surface with implications for polysome organization. Our results provide a foundation for understanding the idiosyncrasies of mycobacterial translation and reveal atomic details of the structure that will facilitate the design of anti-tubercular therapeutics. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

  15. A clinico-pathological study of 70 cases of pemphigus

    Directory of Open Access Journals (Sweden)

    Arya S

    1999-01-01

    Full Text Available A clinicopalhological study of 70 cases of pemphigus observed over a span of four and a half years from January 1992 to June 1996 at the Sir J.J. Group of Hospitals and Grant Medical College, Mumbai is reported. Pemphigus vulgaris constituted the single largest group of 43 cases, followed by pemphigus foliaceus (25 cases and pemphigus vegetans (2 cases. Majority of the cases were seen in the age group of 21-60 years, with a slight male predominance. The youngest patient was 14 years while the eldest was aged 75 years. Mucosal involvement was seen in 31 cases of pemphigus vulgaris, as opposed to only 5 cases of pemphigus foliaceus. Flaccid bullae were present in 100% cases. Pruritus was complained of in 14 cases, though it was more common in pemphigus vegetans and vulgaris. Salient histopathological features of pemphigus vulgaris observed were (I intraepidermal suprabasal blisters (35 cases, (2 presence of acantholytic cells (40 cases, (3 "Row of tombstone appearance" (I8 cases and (4 acantholysis involving follicular sheath (20 cases. Main histopathological features of pemphigus foliaceus were (1 subcorneal blister (15 case, (2 acantholysis (24 cases and (3 bulla cavity containing inflammatory infiltrate (12 cases. Both cases of pemphigus vegetans showed hyperkeratosis, papillomatosis and irregular acanthosis with intra-epidermal eosinophilic abscesses besides suprabasal lacunae.

  16. Piscicides and invertebrates: after 70 years, does anyone really know?

    Science.gov (United States)

    Vinson, M.R.; Dinger, E.C.; Vinson, D.K.

    2010-01-01

    The piscicides rotenone and antimycin have been used for more than 70 years to manage fish populations by eliminating undesirable fish species. The effects of piscicides on aquatic invertebrate assemblages are considered negligible by some and significant by others. This difference of opinion has created contentious situations and delayed native fish restoration projects. We review the scientific evidence and report that short-term ( 1 year) impacts are largely unknown. Recovery of invertebrate assemblages following treatments ranged from a few months for abundances of common taxa to several years for rarer taxa. Variation in reported effects was primarily due to natural variation among species and habitats and a lack of adequate pre- and post-treatment sampling which prevents determining the true impacts to invertebrate assemblages. The factors most likely to influence impacts and recovery of aquatic invertebrate assemblages following piscicide treatments are: (1) concentration, duration, and breadth of the piscicide treatment; (2) invertebrate morphology and life history characteristics, including surface area to volume ratios, type of respiration organs, generation time, and propensity to disperse; (3) refugia presence; and (4) distance from colonization sources.

  17. A 65--70 year oscillation in observed surface temperatures

    International Nuclear Information System (INIS)

    Schlesinger, M.E.; Ramankutty, N.

    1994-01-01

    There are three possible sources for the 65--70-year ''global'' oscillation: (1) random forcing of the ocean by the atmosphere, such as by white noise; (2) external oscillatory forcing of the climate system, such as by a variation in the solar irradiance; and (3) an internal oscillation of the atmosphere-ocean system. It is unlikely that putative variations in solar irradiance are the source of the oscillation because solar forcing should generate a global response, but the oscillation is not global. It is also unlikely that white-noise forcing is the source of the oscillation because such forcing should generate an oceanwide response, but the oscillation is not panoceanic. Consequently, the most probable cause of the oscillation is an internal oscillation of the atmosphere-ocean system. This conclusion is supported by a growing body of observational evidence and coupled atmosphere/ocean general circulation model simulation results. Comparison of the regional and global-mean temperature changes caused by the oscillation with those induced by GHG + ASA forcing shows that the rapid rise in global-mean temperature between about 1908 and 1946, and the subsequent reversal of this warming until about 1965 were the result of the oscillation. In the North Atlantic and North American regions, the domination of the GHG + ASA-induced warming by the oscillation has obscured and confounded detection of this warming

  18. Revalidation studies of Mark 16 experiments: J70

    International Nuclear Information System (INIS)

    Lee, S.Y.

    1993-01-01

    The MGBS-TGAL combination of the J70 criticality modules was validated for Mark 16 lattices by H. K. Clark as reported in DPST-83-1025. Unfortunately, the records of the calculations reported can not be retrieved and the descriptions of the modeling used are not fully provided in DPST-83-1025. The report does not describe in detail how to model the experiments and how to set up the input. The computer output for the cases reported in the memorandum can not be located in files. The MGBS-TGAL calculations reported in DPST-83-1025 have been independently reperformed to provide retrievable record copies of the calculations, to provide a detailed description and discussion of the methodology used, and to serve as a training exercise for a novice criticality safety engineer. The current results reproduce Clark's reported results to within about 0.01% or better. A procedure to perform these and similar calculations is given in this report, with explanation of the methodology choices provided. Copies of the computer output have been made via microfiche and will be maintained in APG files

  19. Igor Germanovich Levichev (in connection with the 70th anniversary

    Directory of Open Access Journals (Sweden)

    R. V. Kamelin

    2016-03-01

    Full Text Available October 6, 2015 Igor Germanovich Levichev, Ph. D., Senior Researcher of the Herbarium of the Komarov Botanical Institute of Russian Academy of Sciences, celebrated his 70th birthday. He is widely known monograph of the large genus Gagea and its related genera as well as the author of the original hypothesis in the evolution of morphological structures of monocots. His scientific career began with expeditions to Chukotka, Kamchatka, in the Trans-Baikal region, Kyzylkum (Uzbekistan, Badkhyz Nature Reserve (Turkmenistan, 1972. Since the autumn of 1972, I. G. Levichev worked in Chatkal nature reserve (Uzbekistan, where he created the basis for the monitoring of vegetation changes in two key areas of relatively little changed Uzbek part of the Western Tien Shan. He worked on the creation of “The Red Book of Uzbek SSR” (1984, “Guide to the Plants of Central Asia. Critical synopsis of Flora “(1987. In 1996 he defended his thesis on the topic “Genus Gagea Salisb. of Western Tien Shan “. In collaboration with colleges from German, Italian and other countries I. G. Levichev published a number of interesting results of molecular phylogenetic studies of the genus Gagea (and its related genera. He also described a new genus – Kharkevichia Levichev of Lloydia – Gagea relationship (the family Liliaceae.. Igor Germanovich is the au­thor of over 120 scientific (scientific and popular works, 53 of them are in fact of Gagea species.

  20. Simulation on Vehicle Vibration Offset of NX70 Flatcar

    Directory of Open Access Journals (Sweden)

    Han Yanhui

    2014-11-01

    Full Text Available The current rolling stock gauge for standard gauge railway is a static gauge to check the vehicle frame. The contradiction of large construction gauge and small rolling stock gauge has always existed. It is important to set down the clearance requirements in respect of physical size for the safe passage of rail vehicles. Reasonably determining the maximum vibration offset can improve the efficiency of clearance. As an example, analyze the complex vibration of NX70 flat car by simulation test on the running track. Comprehensive considering the track model, loading plan, line conditions and running speed, then SIMPACK is used to present the vehicle system dynamics simulation model. After researching simulation result, respectively determine the maximum vehicle vibration offset for railroads of Class I, Class II and Class III on the height of the center of gravity 2000 mm and 2400 mm. According to the clearance between the structure gauge and the position of maximum vibration offset, analyze the safety of vehicle operation since the center of gravity is higher than before.

  1. Lifetimes and magnetic moments in odd-odd 70 As

    International Nuclear Information System (INIS)

    Pantelica, D.; Negoita, F.; Stanoiu, M.

    1998-01-01

    The extensive experimental and theoretical work on the structure of N∼Z, A = 60-80 nuclei revealed many interesting features: large prolate deformations (β = 0.4), strong shape variations as a function of particle number, excitation energy, spin and shape coexistence effects. They are related with drastic changes of properties observed in nuclei with Z≥33 when going from spherical nuclei with N = 50 to neutron deficient nuclei with N = 38 or 40. Both the rapid changes in structure and the shape coexistence appear to reflect the competition between the shell gaps which occur at large oblate and prolate deformations near nucleon numbers 36 and 38 for both protons and neutrons. For N∼Z nuclei the same shell gaps appear simultaneously for both protons and neutrons and reinforce each other. The microscopic structure of the nuclei in the mass region A = 60-80 is essentially determined by the 1g 9/2 , 2p 1/2 , 1f 5/2 and 2p 3/2 orbitals. Because no unique interpretation of the unusual features discovered in these nuclei exists, the systematic experimental study of structure of these nuclei is still an interesting subject. As part of a systematic experimental study undertaken to investigate the structure of neutron deficient, odd-odd As nuclei, 68,70,72 As, the level scheme of 70 As was investigated using heavy ion induced reactions and in-beam γ-ray spectroscopy techniques. At energies between 500 and 900 keV a multiplet of negative parity levels has been observed. At higher energies a high-spin positive parity sequence of levels starting with a E x = 1676 keV, J π 8 + level is strongly populated. Additional information is required in order to establish the structure of low and high-spin levels of both parities. The magnetic moments of the 8 + and 9 + levels have been measured using the time-integral perturbed angular distribution technique and the lifetimes of four levels have been determined using the recoil-distance method. From the measured lifetime for the 9

  2. Mild electrical stimulation with heat stimulation increase heat shock protein 70 in articular chondrocyte.

    Science.gov (United States)

    Hiraoka, Nobuyuki; Arai, Yuji; Takahashi, Kenji A; Mazda, Osam; Kishida, Tsunao; Honjo, Kuniaki; Tsuchida, Shinji; Inoue, Hiroaki; Morino, Saori; Suico, Mary Ann; Kai, Hirofumi; Kubo, Toshikazu

    2013-06-01

    The objective of this study is to investigate the effects of mild electrical stimulation (MES) and heat stress (HS) on heat shock protein 70 (HSP70), that protects chondrocytes and enhances cartilage matrix metabolism, in chondrocyte and articular cartilage. Rabbit articular chondrocytes were treated with MES and/or HS. The safeness was assessed by LDH assay and morphology. HSP70 protein, ubiquitinated proteins and HSP70 mRNA were examined by Western blotting and real-time PCR. Rat knee joints were treated with MES and/or HS. HSP70 protein, ubiquitinated proteins, HSP70 mRNA and proteoglycan core protein (PG) mRNA in articular cartilage were investigated. In vitro, HS increased HSP70 mRNA and HSP70 protein. MES augmented ubiquitinated protein and HSP70 protein, but not HSP70 mRNA. MES + HS raised HSP70 mRNA and ubiquitinated protein, and significantly increased HSP70 protein. In vivo, HS and MES + HS treatment augmented HSP70 mRNA. HS modestly augmented HSP70 protein. MES + HS significantly increased HSP70 protein and ubiquitinated proteins. PG mRNA was markedly raised by MES + HS. This study demonstrated that MES, in combination with HS, increases HSP70 protein in chondrocytes and articular cartilage, and promotes cartilage matrix metabolism in articular cartilage. MES in combination with HS can be a novel physical therapy for osteoarthritis by inducing HSP70 in articular cartilage. Copyright © 2013 Orthopaedic Research Society.

  3. Regulation of homologous recombination repair protein Rad51 by Ku70

    International Nuclear Information System (INIS)

    Du Liqing; Liu Qiang; Wang Yan; Xu Chang; Cao Jia; Fu Yue; Chen Fenghua; Fan Feiyue

    2013-01-01

    Objective: To explore the regulative effect of non-homologous end joining (NHEJ)protein Ku70 on homologous recombination repair protein Rad51, and to investigate the synergistic mechanism of homologous recombination repair in combination with NHEJ. Methods: Observed Rad51 protein expression after transfect Ku70 small interfering RNA or Ku70 plasmid DNA into tumor cells using Western blot. Results: Expression of Rad51 was obviously reduced after pretreated with Ku70 small interfering RNA. And with the increasing expression of Ku70 protein after transfection of Ku70 plasmid DNA PGCsi3.0-hKu70 into tumor cell lines, the Rad51 protein expression was increased. Conclusion: Ku70 protein has regulating effect on gene expression of Rad51, and it might participate in the collaboration between homologous recombination repair and NHEJ. (authors)

  4. Cytosine deletion at AP2-box region of HSP70 promoter and its ...

    Indian Academy of Sciences (India)

    of thermotolerance in cells (Leung et al. 1996). ... region of HSP70 significantly affected cellular thermotol- erance ... The double PCR-RFLP using ScrFI confirmed the occur- ... suade of HSP70 on defense of proteins related to respiration.

  5. EXO70C2 is a key regulatory factor for optimal tip growth of pollen

    KAUST Repository

    Synek, Lukas

    2017-03-30

    The exocyst, an eukaryotic tethering complex, co-regulates targeted exocytosis as an effector of small GTPases in polarized cell growth. In land plants, several exocyst subunits are encoded by double or triple paralogs, culminating in tens of EXO70 paralogs. Out of 23 Arabidopsis EXO70 isoforms, we analyzed seven isoforms expressed in pollen. Genetic and microscopic analyses of single mutants in EXO70A2, C1, C2, F1, H3, H5, and H6 genes revealed that only a loss-of-function EXO70C2 allele resulted in a significant male-specific transmission defect (segregation 40%:51%:9%) due to aberrant pollen tube growth. Mutant pollen tubes grown in vitro exhibited enhanced growth rate and a decreased thickness of the tip cell wall, causing tip bursts. However, exo70C2 pollen tubes could frequently recover and restart their speedy elongation, resulting in a repetitive stop-and-go growth dynamics. A pollen-specific depletion of the closest paralog, EXO70C1, using ami-RNA in the exo70C2 mutant background resulted in a complete pollen-specific transmission defect, suggesting redundant functions of EXO70C1 and EXO70C2. Both EXO70C1 and EXO70C2, GFP-tagged and expressed under their native promoters, localized in the cytoplasm of pollen grains, pollen tubes, and also root trichoblast cells. Expression of EXO70C2-GFP complemented aberrant growth of exo70C2 pollen tubes. The absent EXO70C2 interactions with core exocyst subunits in the yeast two-hybrid assay, cytoplasmic localization, and genetic effect suggest an unconventional EXO70 function possibly as a regulator of exocytosis outside the exocyst complex. In conclusion, EXO70C2 is a novel factor contributing to the regulation of optimal tip growth of Arabidopsis pollen tubes.

  6. Corrosion of heat exchanger materials under heat transfer conditions

    International Nuclear Information System (INIS)

    Tapping, R.L.; Lavoie, P.A.; Disney, D.J.

    1987-01-01

    Severe pitting has occurred in moderator heat exchangers tubed with Incoloy-800 in Pickering Nuclear Generating Station. The pitting originated on the cooling side (outside) of the tubes and perforation occurred in less than two years. It was known from corrosion testing at CRNL that Incoloy-800 was not susceptible to pitting in Lake Ontario water under isothermal conditions. Corrosion testing with heat transfer across the tube wall was carried out, and it was noted that severe pitting could occur under deposits formed on the tubes in silty Lake Ontario water. Subsequent testing, carried out in co-operation with Ontario Hydro Research Division, investigated the pitting resistance of other candidate tubing alloys: Incoloy-825, 904 L stainless steel, AL-6X, Inconel-625, 70:30 Cu:Ni, titanium, Sanicro-30 and Sanicro-28 1 . Of these, only titanium and Sanicro-28 have not suffered some degree of pitting attack in silt-containing Lake Ontario Water. In the absence of silt, and hence deposits, no pitting took place on any of the alloys tested

  7. 40 CFR 407.70 - Applicability; description of the canned and preserved vegetables subcategory.

    Science.gov (United States)

    2010-07-01

    ... canned and preserved vegetables subcategory. 407.70 Section 407.70 Protection of Environment... VEGETABLES PROCESSING POINT SOURCE CATEGORY Canned and Preserved Vegetables Subcategory § 407.70 Applicability; description of the canned and preserved vegetables subcategory. The provisions of this subpart...

  8. 27 CFR 70.162 - Levy and distraint on salary and wages.

    Science.gov (United States)

    2010-04-01

    ... salary and wages. 70.162 Section 70.162 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX... § 70.162 Levy and distraint on salary and wages. (a) Notice of intent to levy. Levy may be made for any... salary or wages. A levy on salary or wages is continuous from the time of the levy until the liability...

  9. 46 CFR 32.70-5 - Hull requirements; general-TB/ALL.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Hull requirements; general-TB/ALL. 32.70-5 Section 32.70..., AND HULL REQUIREMENTS Hull Requirements for Steel Hull Tank Vessels Constructed Prior to November 10, 1936 § 32.70-5 Hull requirements; general—TB/ALL. The scantlings, material, and workmanship, the...

  10. 48 CFR 1252.239-70 - Security requirements for unclassified information technology resources.

    Science.gov (United States)

    2010-10-01

    ... unclassified information technology resources. 1252.239-70 Section 1252.239-70 Federal Acquisition Regulations... of Provisions and Clauses 1252.239-70 Security requirements for unclassified information technology... Unclassified Information Technology Resources (APR 2005) (a) The Contractor shall be responsible for...

  11. 48 CFR 2852.233-70 - Protests filed directly with the Department of Justice.

    Science.gov (United States)

    2010-10-01

    ... with the Department of Justice. 2852.233-70 Section 2852.233-70 Federal Acquisition Regulations System DEPARTMENT OF JUSTICE Clauses and Forms SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 2852.233-70 Protests filed directly with the Department of Justice. As prescribed in 2833.102(d...

  12. 48 CFR 1352.209-70 - Potential organizational conflict of interest.

    Science.gov (United States)

    2010-10-01

    ... and Consultant Conflicts of Interest) due to [state the nature of the potential conflict]. Accordingly... conflict of interest. 1352.209-70 Section 1352.209-70 Federal Acquisition Regulations System DEPARTMENT OF....209-70 Potential organizational conflict of interest. As prescribed in 48 CFR 1309.507-1(a), insert...

  13. 40 CFR 424.70 - Applicability; description of the electrolytic chromium subcategory.

    Science.gov (United States)

    2010-07-01

    ... electrolytic chromium subcategory. 424.70 Section 424.70 Protection of Environment ENVIRONMENTAL PROTECTION... Electrolytic Chromium Subcategory § 424.70 Applicability; description of the electrolytic chromium subcategory. The provisions of this subpart are applicable to discharges resulting from the manufacture of chromium...

  14. 46 CFR 70.05-5 - Specific application noted in text.

    Science.gov (United States)

    2010-10-01

    ... the text pertains, and in many cases limits the application of the text to vessels contracted for... 46 Shipping 3 2010-10-01 2010-10-01 false Specific application noted in text. 70.05-5 Section 70... PROVISIONS Application § 70.05-5 Specific application noted in text. (a) At the beginning of the various...

  15. 48 CFR 1852.203-70 - Display of Inspector General Hotline Posters.

    Science.gov (United States)

    2010-10-01

    ... General Hotline Posters. 1852.203-70 Section 1852.203-70 Federal Acquisition Regulations System NATIONAL... Provisions and Clauses 1852.203-70 Display of Inspector General Hotline Posters. As prescribed in 1803.7001, insert the following clause: Display of Inspector General Hotline Posters (JUN 2001) (a) The Contractor...

  16. 19 CFR 210.70 - Forfeiture or return of complainant's temporary relief bond.

    Science.gov (United States)

    2010-04-01

    ... relief bond. 210.70 Section 210.70 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION INVESTIGATIONS OF UNFAIR PRACTICES IN IMPORT TRADE ADJUDICATION AND ENFORCEMENT Temporary Relief § 210.70... and the Commission will be guided by practice under Rule 65 of the Federal Rules of Civil Procedure...

  17. 48 CFR 1335.016-70 - DOC procedures for the use of broad agency announcements.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false DOC procedures for the use of broad agency announcements. 1335.016-70 Section 1335.016-70 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE SPECIAL CATEGORIES OF CONTRACTING RESEARCH AND DEVELOPMENT CONTRACTING 1335.016-70 DOC...

  18. 48 CFR 2917.504-70 - Signature authority and internal procedures.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Signature authority and internal procedures. 2917.504-70 Section 2917.504-70 Federal Acquisition Regulations System DEPARTMENT OF... The Economy Act 2917.504-70 Signature authority and internal procedures. (a) A DOL contracting officer...

  19. 28 CFR 70.20 - Purpose of financial and program management.

    Science.gov (United States)

    2010-07-01

    ..., HOSPITALS AND OTHER NON-PROFIT ORGANIZATIONS Post-Award Requirements Financial and Program Management § 70.20 Purpose of financial and program management. Sections 70.21 through 70.28 prescribe standards for financial management systems, methods for making payments and rules for: Satisfying cost sharing and...

  20. 30 CFR 800.70 - Bonding for anthracite operations in Pennsylvania.

    Science.gov (United States)

    2010-07-01

    ... Pennsylvania. 800.70 Section 800.70 Mineral Resources OFFICE OF SURFACE MINING RECLAMATION AND ENFORCEMENT... REGULATORY PROGRAMS § 800.70 Bonding for anthracite operations in Pennsylvania. (a) All of the provisions of... operations in Pennsylvania except that— (1) Specified bond limits shall be determined by the regulatory...

  1. 48 CFR 1415.406-70 - Department of the Interior price negotiation memorandum (PNM).

    Science.gov (United States)

    2010-10-01

    ... price negotiation memorandum (PNM). 1415.406-70 Section 1415.406-70 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 1415.406-70 Department of the Interior price negotiation memorandum (PNM). (a) Policy. In...

  2. 49 CFR 1242.70 - Fringe benefits (account 12-52-00).

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 9 2010-10-01 2010-10-01 false Fringe benefits (account 12-52-00). 1242.70 Section 1242.70 Transportation Other Regulations Relating to Transportation (Continued) SURFACE...-Transportation § 1242.70 Fringe benefits (account 12-52-00). Separate common expenses in proportion to the...

  3. 36 CFR 1256.70 - What controls access to national security-classified information?

    Science.gov (United States)

    2010-07-01

    ... national security-classified information? 1256.70 Section 1256.70 Parks, Forests, and Public Property... HISTORICAL MATERIALS Access to Materials Containing National Security-Classified Information § 1256.70 What controls access to national security-classified information? (a) The declassification of and public access...

  4. 33 CFR 110.70a - Northeast River, North East, Md.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Northeast River, North East, Md. 110.70a Section 110.70a Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.70a Northeast River, North East, Md. The water...

  5. 48 CFR 1652.203-70 - Misleading, deceptive, or unfair advertising.

    Science.gov (United States)

    2010-10-01

    ... unfair advertising. 1652.203-70 Section 1652.203-70 Federal Acquisition Regulations System OFFICE OF... CLAUSES Texts of FEHBP Clauses 1652.203-70 Misleading, deceptive, or unfair advertising. As prescribed in... Unfair Advertising (JAN 1991) (a) The Carrier agrees that any advertising material, including that...

  6. 48 CFR 225.802-70 - Contracts for performance outside the United States and Canada.

    Science.gov (United States)

    2010-10-01

    ... outside the United States and Canada. 225.802-70 Section 225.802-70 Federal Acquisition Regulations System... States and Canada. Follow the procedures at PGI 225.802-70 when placing a contract requiring performance outside the United States and Canada. Also see Subpart 225.74, Defense Contractors Outside the United...

  7. 48 CFR 1019.202-70 - The Treasury Mentor-Protégé Program.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false The Treasury Mentor-Protégé Program. 1019.202-70 Section 1019.202-70 Federal Acquisition Regulations System DEPARTMENT OF THE TREASURY SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 1019.202-70 The Treasury Mentor...

  8. 48 CFR 970.3102-05-30-70 - Patent costs and technology transfer costs.

    Science.gov (United States)

    2010-10-01

    ... technology transfer costs. 970.3102-05-30-70 Section 970.3102-05-30-70 Federal Acquisition Regulations System... Principles and Procedures 970.3102-05-30-70 Patent costs and technology transfer costs. (a) For management and operating contracts that do not include the clause at 970.5227-3, Technology Transfer Mission, the...

  9. 24 CFR 1007.70 - Disqualification of lenders and civil money penalties.

    Science.gov (United States)

    2010-04-01

    ... civil money penalties. 1007.70 Section 1007.70 Housing and Urban Development Regulations Relating to....70 Disqualification of lenders and civil money penalties. (a) In general—(1) Grounds for action. HUD... or holder that are guaranteed under this part. (b) Civil money penalties for intentional violations...

  10. 48 CFR 1852.214-70 - Caution to offerors furnishing descriptive literature.

    Science.gov (United States)

    2010-10-01

    ... furnishing descriptive literature. 1852.214-70 Section 1852.214-70 Federal Acquisition Regulations System... CLAUSES Texts of Provisions and Clauses 1852.214-70 Caution to offerors furnishing descriptive literature... Descriptive Literature (DEC 1988) Bidders are cautioned against furnishing as a part of their bids descriptive...

  11. 48 CFR 852.233-70 - Protest content/alternative dispute resolution.

    Science.gov (United States)

    2010-10-01

    .../alternative dispute resolution. 852.233-70 Section 852.233-70 Federal Acquisition Regulations System... Provisions and Clauses § 852.233-70 Protest content/alternative dispute resolution. As prescribed in 833.106, insert the following provision: Protest Content/Alternative Dispute Resolution (JAN 2008) (a) Any protest...

  12. 27 CFR 70.304 - Place for filing documents other than returns.

    Science.gov (United States)

    2010-04-01

    ...: Members of the taxpayer's family, an employee of the taxpayer, the taxpayer's attorney, accountant, or tax... other than returns. 70.304 Section 70.304 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX... ADMINISTRATION Collection of Excise and Special (Occupational) Tax Miscellaneous Provisions § 70.304 Place for...

  13. 40 CFR 471.70 - Applicability; description of the uranium forming subcategory.

    Science.gov (United States)

    2010-07-01

    ... uranium forming subcategory. 471.70 Section 471.70 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS NONFERROUS METALS FORMING AND METAL POWDERS POINT SOURCE CATEGORY Uranium Forming Subcategory § 471.70 Applicability; description of the uranium forming...

  14. EXO70C2 is a key regulatory factor for optimal tip growth of pollen

    KAUST Repository

    Synek, Lukas; Vukasinovic, Nemanja; Kulich, Ivan; Hala, Michal; Aldorfova, Klara; Fendrych, Matyas; Zarsky, Viktor

    2017-01-01

    paralogs. Out of 23 Arabidopsis EXO70 isoforms, we analyzed seven isoforms expressed in pollen. Genetic and microscopic analyses of single mutants in EXO70A2, C1, C2, F1, H3, H5, and H6 genes revealed that only a loss-of-function EXO70C2 allele resulted

  15. Evidence of icosahedral short-range order in Zr70Cu30 and Zr70Cu29Pd1 metallic glasses

    DEFF Research Database (Denmark)

    Saksl, K.; Franz, H.; Jovari, P.

    2003-01-01

    Change in local atomic environment during crystallization of Zr-based glassy alloys was studied by extended x-ray absorption fine structure (EXAFS) spectroscopy. The formation of icosahedral quasicrystalline phase followed by crystallization of tetragonal CuZr2 has been observed in the Zr70Cu29Pd1...... glassy alloy during annealing up to 850 K. On the other hand, the binary Zr70Cu30 alloy shows a single glassy to crystalline CuZr2 phase transformation. The local atomic environment of as-quenched Zr70Cu30 alloy is matched to an icosahedral local atomic configuration, which is similar to that of the as......-quenched Zr70Cu29Pd1 alloy and the alloy annealed at 593 K containing icosahedral phase. Considering that the supercooled liquid region appears prior to crystallization in the Zr70Cu30 glassy alloy, the observed results support the theory claiming a strong correlation between the existence of local...

  16. Neuropatologia da cinomose canina: 70 casos (2005-2008

    Directory of Open Access Journals (Sweden)

    Marcia C. Silva

    2009-08-01

    Full Text Available Este estudo teve como objetivo realizar uma investigação anátomo-patológica detalhada das lesões e sua distribuição no sistema nervoso central (SNC de cães com cinomose. Foram avaliadas secções padronizadas do encéfalo e da medula espinhal de 70 cães. Os casos foram agrupados de acordo com a idade dos cães e classificados conforme a evolução das lesões. Os resultados permitem concluir que: (1 encefalomielite induzida pelo vírus da cinomose canina é mais prevalente em filhotes e adultos; (2 lesões macroscópicas no SNC ocorrem com baixa freqüência; (3 o encéfalo é mais acometido do que a medula espinhal; (4 as cinco regiões anatômicas mais afetadas do encéfalo são, em ordem decrescente de freqüência, o cerebelo, o diencéfalo, o lobo frontal, a ponte e o mesencéfalo; (5 a região anatômica mais afetada da medula espinhal é o segmento cervical cranial (C1-C5; (6 lesões subagudas e crônicas são mais comuns do que lesões agudas; (7 desmielinização é a lesão mais prevalente e ocorre principalmente no cerebelo, na ponte e no diencéfalo, quase sempre acompanhada de astrogliose e inflamação não-supurativa; (8 na maior parte dos casos em que há astrogliose, observam-se astrócitos gemistocíticos, freqüentemente com formação de sincícios; (9 leptomeningite não-supurativa, malacia e necrose cortical laminar são lesões relativamente freqüentes no encéfalo, mas não na medula espinhal; (10 corpúsculos de inclusão no encéfalo são muito comuns, ocorrem principalmente em astrócitos e com freqüência menor em neurônios; no entanto, independentemente da célula afetada, são vistos predominantemente no núcleo; (11 uma classificação da encefalite na cinomose com base em síndromes clínicas relacionadas com a idade do cão é imprecisa.The current study was performed to determine the pathology and distribution of lesions in the central nervous system (CNS of 70 dogs naturally affected by canine

  17. 70-kDa Heat Shock Cognate Protein hsc70 Mediates Calmodulin-dependent Nuclear Import of the Sex-determining Factor SRY*

    Science.gov (United States)

    Kaur, Gurpreet; Lieu, Kim G.; Jans, David A.

    2013-01-01

    We recently showed that the developmentally important family of SOX (SRY (sex determining region on the Y chromosome)-related high mobility group (HMG) box) proteins require the calcium-binding protein calmodulin (CaM) for optimal nuclear accumulation, with clinical mutations in SRY that specifically impair nuclear accumulation via this pathway resulting in XY sex reversal. However, the mechanism by which CaM facilitates nuclear accumulation is unknown. Here, we show, for the first time, that the 70-kDa heat shock cognate protein hsc70 plays a key role in CaM-dependent nuclear import of SRY. Using a reconstituted nuclear import assay, we show that antibodies to hsc70 significantly reduce nuclear accumulation of wild type SRY and mutant derivatives thereof that retain CaM-dependent nuclear import, with an increased rate of nuclear accumulation upon addition of both CaM and hsc70, in contrast to an SRY mutant derivative with impaired CaM binding. siRNA knockdown of hsc70 in intact cells showed similar results, indicating clear dependence upon hsc70 for CaM-dependent nuclear import. Analysis using the technique of fluorescence recovery after photobleaching indicated that hsc70 is required for the maximal rate of SRY nuclear import in living cells but has no impact upon SRY nuclear retention/nuclear dynamics. Finally, we demonstrate direct binding of hsc70 to the SRY·CaM complex, with immunoprecipitation experiments from cell extracts showing association of hsc70 with wild type SRY, but not with a mutant derivative with impaired CaM binding, dependent on Ca2+. Our novel findings strongly implicate hsc70 in CaM-dependent nuclear import of SRY. PMID:23235156

  18. The Welsh Blood Service - 70 years of continuous change.

    Science.gov (United States)

    Poole, G D

    2017-06-01

    would become ever more important, although the impact of this was lessened somewhat by a decreasing demand for blood following the Better Blood Transfusion initiatives. The Medicines and Healthcare Products Regulatory Agency found new powers and impetus after the so-called EU Blood Directive was transposed into British law, and the inspection regime became significantly more onerous, requiring more resources to be put in this area. A strategic review found that some parts of the service required extensive modernisation, such as the information technology system in use and the deployment of staff on the blood collection teams, and these matters were attended to. The review also prompted the Welsh Government to consider the question of blood supply to and blood collection in North Wales, which had been the remit of the Liverpool Centre since the establishment of the NBTS in 1946. The Welsh Government duly announced the creation of an all-Wales Blood Service, and finally, in May 2016, 70 years after its creation, the WBS took over responsibility for blood collection and blood provision in the whole of Wales. © 2017 British Blood Transfusion Society.

  19. Ku70 inhibits gemcitabine-induced DNA damage and pancreatic cancer cell apoptosis

    International Nuclear Information System (INIS)

    Ma, Jiali; Hui, Pingping; Meng, Wenying; Wang, Na; Xiang, Shihao

    2017-01-01

    The current study focused on the role of Ku70, a DNA-dependent protein kinase (DNA-PK) complex protein, in pancreatic cancer cell resistance to gemcitabine. In both established cell lines (Mia-PaCa-2 and PANC-1) and primary human pancreatic cancer cells, shRNA/siRNA-mediated knockdown of Ku70 significantly sensitized gemcitabine-induced cell death and proliferation inhibition. Meanwhile, gemcitabine-induced DNA damage and subsequent pancreatic cancer cell apoptosis were also potentiated with Ku70 knockdown. On the other hand, exogenous overexpression of Ku70 in Mia-PaCa-2 cells suppressed gemcitabine-induced DNA damage and subsequent cell apoptosis. In a severe combined immune deficient (SCID) mice Mia-PaCa-2 xenograft model, gemcitabine-induced anti-tumor activity was remarkably pontificated when combined with Ku70 shRNA knockdown in the xenografts. The results of this preclinical study imply that Ku70 might be a primary resistance factor of gemcitabine, and Ku70 silence could significantly chemo-sensitize gemcitabine in pancreatic cancer cells. - Highlights: • Ku70 knockdown sensitizes gemcitabine-induced killing of pancreatic cancer cells. • Ku70 knockdown facilitates gemcitabine-induced DNA damage and cell apoptosis. • Ku70 overexpression deceases gemcitabine's sensitivity in pancreatic cancer cells. • Ku70 knockdown sensitizes gemcitabine-induced anti-tumor activity in vivo.

  20. Investigating the Chaperone Properties of a Novel Heat Shock Protein, Hsp70.c, from Trypanosoma brucei

    Directory of Open Access Journals (Sweden)

    Adélle Burger

    2014-01-01

    Full Text Available The neglected tropical disease, African Trypanosomiasis, is fatal and has a crippling impact on economic development. Heat shock protein 70 (Hsp70 is an important molecular chaperone that is expressed in response to stress and Hsp40 acts as its co-chaperone. These proteins play a wide range of roles in the cell and they are required to assist the parasite as it moves from a cold blooded insect vector to a warm blooded mammalian host. A novel cytosolic Hsp70, from Trypanosoma brucei, TbHsp70.c, contains an acidic substrate binding domain and lacks the C-terminal EEVD motif. The ability of a cytosolic Hsp40 from Trypanosoma brucei J protein 2, Tbj2, to function as a co-chaperone of TbHsp70.c was investigated. The main objective was to functionally characterize TbHsp70.c to further expand our knowledge of parasite biology. TbHsp70.c and Tbj2 were heterologously expressed and purified and both proteins displayed the ability to suppress aggregation of thermolabile MDH and chemically denatured rhodanese. ATPase assays revealed a 2.8-fold stimulation of the ATPase activity of TbHsp70.c by Tbj2. TbHsp70.c and Tbj2 both demonstrated chaperone activity and Tbj2 functions as a co-chaperone of TbHsp70.c. In vivo heat stress experiments indicated upregulation of the expression levels of TbHsp70.c.

  1. BAG3 Is a Modular, Scaffolding Protein that physically Links Heat Shock Protein 70 (Hsp70) to the Small Heat Shock Proteins.

    Science.gov (United States)

    Rauch, Jennifer N; Tse, Eric; Freilich, Rebecca; Mok, Sue-Ann; Makley, Leah N; Southworth, Daniel R; Gestwicki, Jason E

    2017-01-06

    Small heat shock proteins (sHsps) are a family of ATP-independent molecular chaperones that are important for binding and stabilizing unfolded proteins. In this task, the sHsps have been proposed to coordinate with ATP-dependent chaperones, including heat shock protein 70 (Hsp70). However, it is not yet clear how these two important components of the chaperone network are linked. We report that the Hsp70 co-chaperone, BAG3, is a modular, scaffolding factor to bring together sHsps and Hsp70s. Using domain deletions and point mutations, we found that BAG3 uses both of its IPV motifs to interact with sHsps, including Hsp27 (HspB1), αB-crystallin (HspB5), Hsp22 (HspB8), and Hsp20 (HspB6). BAG3 does not appear to be a passive scaffolding factor; rather, its binding promoted de-oligomerization of Hsp27, likely by competing for the self-interactions that normally stabilize large oligomers. BAG3 bound to Hsp70 at the same time as Hsp22, Hsp27, or αB-crystallin, suggesting that it might physically bring the chaperone families together into a complex. Indeed, addition of BAG3 coordinated the ability of Hsp22 and Hsp70 to refold denatured luciferase in vitro. Together, these results suggest that BAG3 physically and functionally links Hsp70 and sHsps. Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. Role of membrane Hsp70 in radiation sensitivity of tumor cells

    International Nuclear Information System (INIS)

    Murakami, Naoya; Kühnel, Annett; Schmid, Thomas E.; Ilicic, Katarina; Stangl, Stefan; Braun, Isabella S.; Gehrmann, Mathias; Molls, Michael; Itami, Jun; Multhoff, Gabriele

    2015-01-01

    The major stress-inducible heat shock protein 70 (Hsp70) is frequently overexpressed in the cytosol and integrated in the plasma membrane of tumor cells via lipid anchorage. Following stress such as non-lethal irradiation Hsp70 synthesis is up-regulated. Intracellular located Hsp70 is known to exert cytoprotective properties, however, less is known about membrane (m)Hsp70. Herein, we investigate the role of mHsp70 in the sensitivity towards irradiation in tumor sublines that differ in their cytosolic and/or mHsp70 levels. The isogenic human colon carcinoma sublines CX + with stable high and CX − with stable low expression of mHsp70 were generated by fluorescence activated cell sorting, the mouse mammary carcinoma sublines 4 T1 (4 T1 ctrl) and Hsp70 knock-down (4 T1 Hsp70 KD) were produced using the CRISPR/Cas9 system, and the Hsp70 down-regulation in human lung carcinoma sublines H1339 ctrl/H1339 HSF-1 KD and EPLC-272H ctrl/EPLC-272H HSF-1 KD was achieved by small interfering (si)RNA against Heat shock factor 1 (HSF-1). Cytosolic and mHsp70 was quantified by Western blot analysis/ELISA and flow cytometry; double strand breaks (DSBs) and apoptosis were measured by flow cytometry using antibodies against γH2AX and real-time PCR (RT-PCR) using primers and antibodies directed against apoptosis related genes; and radiation sensitivity was determined using clonogenic cell surviving assays. CX + /CX − tumor cells exhibited similar cytosolic but differed significantly in their mHsp70 levels, 4 T1 ctrl/4 T1 Hsp70 KD cells showed significant differences in their cytosolic and mHsp70 levels and H1339 ctrl/H1339 HSF-1 KD and EPLC-272H ctrl/EPLC-272H HSF-1 KD lung carcinoma cell sublines had similar mHsp70 but significantly different cytosolic Hsp70 levels. γH2AX was significantly up-regulated in irradiated CX − and 4 T1 Hsp70 KD with low basal mHsp70 levels, but not in their mHsp70 high expressing counterparts, irrespectively of their cytosolic Hsp70 content. After

  3. Tumor imaging and targeting potential of an Hsp70-derived 14-mer peptide.

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    Mathias Gehrmann

    Full Text Available We have previously used a unique mouse monoclonal antibody cmHsp70.1 to demonstrate the selective presence of a membrane-bound form of Hsp70 (memHsp70 on a variety of leukemia cells and on single cell suspensions derived from solid tumors of different entities, but not on non-transformed cells or cells from corresponding 'healthy' tissue. This antibody can be used to image tumors in vivo and target them for antibody-dependent cellular cytotoxicity. Tumor-specific expression of memHsp70 therefore has the potential to be exploited for theranostic purposes. Given the advantages of peptides as imaging and targeting agents, this study assessed whether a 14-mer tumor penetrating peptide (TPP; TKDNNLLGRFELSG, the sequence of which is derived from the oligomerization domain of Hsp70 which is expressed on the cell surface of tumor cells, can also be used for targeting membrane Hsp70 positive (memHsp70+ tumor cells, in vitro.The specificity of carboxy-fluorescein (CF- labeled TPP (TPP to Hsp70 was proven in an Hsp70 knockout mammary tumor cell system. TPP specifically binds to different memHsp70+ mouse and human tumor cell lines and is rapidly taken up via endosomes. Two to four-fold higher levels of CF-labeled TPP were detected in MCF7 (82% memHsp70+ and MDA-MB-231 (75% memHsp70+ cells compared to T47D cells (29% memHsp70+ that exhibit a lower Hsp70 membrane positivity. After 90 min incubation, TPP co-localized with mitochondrial membranes in memHsp70+ tumors. Although there was no evidence that any given vesicle population was specifically localized, fluorophore-labeled cmHsp70.1 antibody and TPP preferentially accumulated in the proximity of the adherent surface of cultured cells. These findings suggest a potential association between membrane Hsp70 expression and cytoskeletal elements that are involved in adherence, the establishment of intercellular synapses and/or membrane reorganization.This study demonstrates the specific binding and rapid

  4. Interaction of caveolin-1 with Ku70 inhibits Bax-mediated apoptosis.

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    Huafei Zou

    Full Text Available Caveolin-1, the structural protein component of caveolae, acts as a scaffolding protein that functionally regulates signaling molecules. We show that knockdown of caveolin-1 protein expression enhances chemotherapeutic drug-induced apoptosis and inhibits long-term survival of colon cancer cells. In vitro studies demonstrate that caveolin-1 is a novel Ku70-binding protein, as shown by the binding of the scaffolding domain of caveolin-1 (amino acids 82-101 to the caveolin-binding domain (CBD of Ku70 (amino acids 471-478. Cell culture data show that caveolin-1 binds Ku70 after treatment with chemotherapeutic drugs. Mechanistically, we found that binding of caveolin-1 to Ku70 inhibits the chemotherapeutic drug-induced release of Bax from Ku70, activation of Bax, translocation of Bax to mitochondria and apoptosis. Potentiation of apoptosis by knockdown of caveolin-1 protein expression is greatly reduced in the absence of Bax expression. Finally, we found that overexpression of wild type Ku70, but not a mutant form of Ku70 that cannot bind to caveolin-1 (Ku70 Φ→A, limits the chemotherapeutic drug-induced Ku70/Bax dissociation and apoptosis. Thus, caveolin-1 acts as an anti-apoptotic protein in colon cancer cells by binding to Ku70 and inhibiting Bax-dependent cell death.

  5. Proteomics shows Hsp70 does not bind peptide sequences indiscriminately in vivo

    International Nuclear Information System (INIS)

    Grossmann, Michael E.; Madden, Benjamin J.; Gao, Fan; Pang, Yuan-Ping; Carpenter, John E.; McCormick, Daniel; Young, Charles Y.F.

    2004-01-01

    Heat shock protein 70 (Hsp70) binds peptide and has several functions that include protein folding, protein trafficking, and involvement with immune function. However, endogenous Hsp70-binding peptides had not previously been identified. Therefore, we eluted and identified several hundred endogenously bound peptides from Hsp70 using liquid chromatography ion trap mass spectrophotometry (LC-ITMS). Our work shows that the peptides are capable of binding Hsp70 as previously described. They are generally 8-26 amino acids in length and correspond to specific regions of many proteins. Through computationally assisted analysis of peptides eluted from Hsp70 we determined variable amino acid sequences, including a 5 amino acid core sequence that Hsp70 favorably binds. We also developed a computer algorithm that predicts Hsp70 binding within proteins. This work helps to define what peptides are bound by Hsp70 in vivo and suggests that Hsp70 facilitates peptide selection by aiding a funneling mechanism that is flexible but allows only a limited number of peptides to be processed

  6. HIF-1α-induced HSP70 regulates anabolic responses in articular chondrocytes under hypoxic conditions.

    Science.gov (United States)

    Tsuchida, Shinji; Arai, Yuji; Takahashi, Kenji A; Kishida, Tsunao; Terauchi, Ryu; Honjo, Kuniaki; Nakagawa, Shuji; Inoue, Hiroaki; Ikoma, Kazuya; Ueshima, Keiichiro; Matsuki, Tomohiro; Mazda, Osam; Kubo, Toshikazu

    2014-08-01

    We assessed whether heat shock protein 70 (HSP70) is involved in hypoxia inducible factor 1 alpha (HIF-1α)-dependent anabolic pathways in articular chondrocytes under hypoxic conditions. Primary rabbit chondrocytes were cultured under normoxia (20% oxygen condition) or hypoxia (1% oxygen condition). Alternatively, cells cultured under normoxia were treated with CoCl2 , which induces HIF-1α, to simulate hypoxia, or transfected with siRNAs targeting HIF-1α (si-HIF-1α) and HSP70 (si-HSP70) under hypoxia. HSP70 expression was enhanced by the increased expression of HIF-1α under hypoxia or simulated hypoxia, but not in the presence of si-HIF-1α. Hypoxia-induced overexpression of ECM genes was significantly suppressed by si-HIF-1α or si-HSP70. Cell viability positively correlated with hypoxia, but transfection with si-HIF-1α or si-HSP70 abrogated the chondroprotective effects of hypoxia. Although LDH release from sodium nitroprusside-treated cells and the proportion of TUNEL positive cells were decreased under hypoxia, transfection with si-HIF-1α or si-HSP70 almost completely blocked these effects. These findings indicated that HIF-1α-induced HSP70 overexpression increased the expression levels of ECM genes and cell viability, and protected chondrocytes from apoptosis. HIF-1α may regulate the anabolic effects of chondrocytes under hypoxic conditions by regulating HSP70 expression. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

  7. PREFERENTIAL SECRETION OF INDUCIBLE HSP70 BY VITILIGO MELANOCYTES UNDER STRESS

    Science.gov (United States)

    Mosenson, Jeffrey A.; Flood, Kelsey; Klarquist, Jared; Eby, Jonathan M.; Koshoffer, Amy; Boissy, Raymond E.; Overbeck, Andreas; C.Tung, Rebecca; Poole, I. Caroline Le

    2014-01-01

    SUMMARY Inducible HSP70 (HSP70i) chaperones peptides from stressed cells, protecting them from apoptosis. Upon extracellular release, HSP70i serves an adjuvant function, enhancing immune responses to bound peptides. We questioned whether HSP70i differentially protects control and vitiligo melanocytes from stress and subsequent immune responses. We compared expression of HSP70i in skin samples, evaluated the viability of primary vitiligo and control melanocytes exposed to bleaching phenols, and measured secreted HSP70i. We determined whether HSP70i traffics to melanosomes to contact immunogenic proteins by cell fractionation, western blotting, electron microscopy and confocal microscopy. Viability of vitiligo and control melanocytes was equally affected under stress. However, vitiligo melanocytes secreted increased amounts of HSP70i in response to MBEH, corroborating with aberrant HSP70i expression in patient skin. Intracellular HSP70i colocalized with melanosomes, and more so in response to MBEH in vitiligo melanocytes. Thus whereas either agent is cytotoxic to melanocytes, MBEH preferentially induces immune responses to melanocytes. PMID:24354861

  8. Functional analysis of the Hikeshi-like protein and its interaction with HSP70 in Arabidopsis

    Energy Technology Data Exchange (ETDEWEB)

    Koizumi, Shinya; Ohama, Naohiko; Mizoi, Junya [Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan); Shinozaki, Kazuo [RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa 230-0045 (Japan); Yamaguchi-Shinozaki, Kazuko, E-mail: akys@mail.ecc.u-tokyo.ac.jp [Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan)

    2014-07-18

    Highlights: • HKL, a Hikeshi homologous gene is identified in Arabidopsis. • HKL interacts with two HSP70 isoforms and regulates the subcellular localization of HSC70-1. • The two HSP70 translocate into nucleus in response to heat stress. • Overexpression of HKL confers thermotolerance in transgenic plants. - Abstract: Heat shock proteins (HSPs) refold damaged proteins and are an essential component of the heat shock response. Previously, the 70 kDa heat shock protein (HSP70) has been reported to translocate into the nucleus in a heat-dependent manner in many organisms. In humans, the heat-induced translocation of HSP70 requires the nuclear carrier protein Hikeshi. In the Arabidopsis genome, only one gene encodes a protein with high homology to Hikeshi, and we named this homolog Hikeshi-like (HKL) protein. In this study, we show that two Arabidopsis HSP70 isoforms accumulate in the nucleus in response to heat shock and that HKL interacts with these HSP70s. Our histochemical analysis revealed that HKL is predominantly expressed in meristematic tissues, suggesting the potential importance of HKL during cell division in Arabidopsis. In addition, we show that HKL regulates HSP70 localization, and HKL overexpression conferred thermotolerance to transgenic Arabidopsis plants. Our results suggest that HKL plays a positive role in the thermotolerance of Arabidopsis plants and cooperatively interacts with HSP70.

  9. Identification and in silico analysis of the Citrus HSP70 molecular chaperone gene family

    Directory of Open Access Journals (Sweden)

    Luciano G. Fietto

    2007-01-01

    Full Text Available The completion of the genome sequencing of the Arabidopsis thaliana model system provided a powerful molecular tool for comparative analysis of gene families present in the genome of economically relevant plant species. In this investigation, we used the sequences of the Arabidopsis Hsp70 gene family to identify and annotate the Citrus Hsp70 genes represented in the CitEST database. Based on sequence comparison analysis, we identified 18 clusters that were further divided into 5 subgroups encoding four mitochondrial mtHsp70s, three plastid csHsp70s, one ER luminal Hsp70 BiP, two HSP110/SSE-related proteins and eight cytosolic Hsp/Hsc70s. We also analyzed the expression profile by digital Northern of each Hsp70 transcript in different organs and in response to stress conditions. The EST database revealed a distinct population distribution of Hsp70 ESTs among isoforms and across the organs surveyed. The Hsp70-5 isoform was highly expressed in seeds, whereas BiP, mitochondrial and plastid HSp70 mRNAs displayed a similar expression profile in the organs analyzed, and were predominantly represented in flowers. Distinct Hsp70 mRNAs were also differentially expressed during Xylella infection and Citrus tristeza viral infection as well as during water deficit. This in silico study sets the groundwork for future investigations to fully characterize functionally the Citrus Hsp70 family and underscores the relevance of Hsp70s in response to abiotic and biotic stresses in Citrus.

  10. FOREWORD: The 70th birthday of Professor Stig Stenholm The 70th birthday of Professor Stig Stenholm

    Science.gov (United States)

    Suominen, Kalle-Antti

    2010-09-01

    information [7] and in Stockholm he had, again, very successful postdocs such as Ulf Leonhardt. Finally, in 2005, Stig Stenholm retired, although he is still active, writing papers, taking part in conferences and making research visits. We honoured his 70th birthday at the CEWQO2009 conference, and hope that the future provides us with further opportunities for such events. Looking at the obituary of Dirk ter Haar, I see that his style with students reminds me of Stig's approach. In my opinion, Stig expects independence and initiative from a student, giving perhaps a broad topic in which the student is expected to find his or her own way, whilst working perhaps with a postdoc. Juha Javanainen has talked about the 'sink or swim' style (not referring to Stig, though). There is a famous series of children's books about Moomin trolls by Tove Jansson (another Swedish-speaking Finn like Stig). In one of them, the Moomin find in early spring a small flower in a patch of land uncovered by snow, pushing its way up. One of them wants to cover it against frost during the night, but another says 'Don't, it'll fare better later if it has some difficulties at first'. At CEWQO2009 Stig gave the full list of his finished PhD students: Rainer Salomaa (1973), Temba Dlodlo (1980), Juha Javanainen (1980), Markus Lindberg (1985), Matti Kaivola (1985), Birger Ståhlberg (1985), Kalle-Antti Suominen (1992), Mackillo Kira (1995), Päivi Törmä (1996), Asta Paloviita (1997), Patrik Öhberg (1998), Martti Havukainen (1999), Erika Andersson (2000), Pawel Piwnicki (2001), Åsa Larson (2001), Markku Jääskeläinen (2003), and Jonas Larson (2005). One should also mention Erkki Kyrölä, who eventually graduated at Rochester and Olli Serimaa, who never graduated but published some important early-stage laser cooling work. As a final note I must mention a passion that Stig and I share, namely books. I have nearly 400 professional physics and mathematics books, but I am certain that the size of Stig

  11. Cognitive Function and Heat Shock Protein 70 in Children With Temporal Lobe Epilepsy.

    Science.gov (United States)

    Oraby, Azza M; Raouf, Ehab R Abdol; El-Saied, Mostafa M; Abou-Khadra, Maha K; Helal, Suzette I; Hashish, Adel F

    2017-01-01

    We conducted the present study to examine cognitive function and serum heat shock protein 70 levels among children with temporal lobe epilepsy. The Stanford-Binet Intelligence Test was carried out to examine cognitive function in 30 children with temporal lobe epilepsy and 30 controls. Serum heat shock protein 70 levels were determined with an enzyme-linked immunosorbent assay. The epilepsy group had significantly lower cognitive function testing scores and significantly higher serum heat shock protein 70 levels than the control group; there were significant negative correlations between serum heat shock protein 70 levels and short-term memory and composite scores. Children with uncontrolled seizures had significantly lower verbal reasoning scores and significantly higher serum heat shock protein 70 levels than children with controlled seizures. Children with temporal lobe epilepsy have cognitive dysfunction and elevated levels of serum heat shock protein 70, which may be considered a stress biomarker.

  12. Molecular analysis of Hsp70 mechanisms in plants and their function in response to stress.

    Science.gov (United States)

    Usman, Magaji G; Rafii, Mohd Y; Martini, Mohammad Y; Yusuff, Oladosu A; Ismail, Mohd R; Miah, Gous

    2017-04-01

    Studying the strategies of improving abiotic stress tolerance is quite imperative and research under this field will increase our understanding of response mechanisms to abiotic stress such as heat. The Hsp70 is an essential regulator of protein having the tendency to maintain internal cell stability like proper folding protein and breakdown of unfolded proteins. Hsp70 holds together protein substrates to help in movement, regulation, and prevent aggregation under physical and or chemical pressure. However, this review reports the molecular mechanism of heat shock protein 70 kDa (Hsp70) action and its structural and functional analysis, research progress on the interaction of Hsp70 with other proteins and their interaction mechanisms as well as the involvement of Hsp70 in abiotic stress responses as an adaptive defense mechanism.

  13. The role of HSP70 in mediating age-dependent mortality in sepsis

    Science.gov (United States)

    McConnell, Kevin W.; Fox, Amy C.; Clark, Andrew T.; Chang, Nai-Yuan Nicholas; Dominguez, Jessica A.; Farris, Alton B.; Buchman, Timothy G.; Hunt, Clayton R.; Coopersmith, Craig M.

    2011-01-01

    Sepsis is primarily a disease of the aged, with increased incidence and mortality occurring in aged hosts. Heat shock protein (HSP) 70 plays an important role in both healthy aging and the stress response to injury. The purpose of this study was to determine the role of HSP70 in mediating mortality and the host inflammatory response in aged septic hosts. Sepsis was induced in both young (6–12week old) and aged (16–17 month old) HSP70−/− and wild type (WT) mice to determine if HSP70 modulated outcome in an age-dependent fashion. Young HSP70−/− and WT mice subjected to cecal ligation and puncture (CLP), Pseudomonas aeruginosa pneumonia or Streptococcus pneumoniae pneumonia had no differences in mortality, suggesting HSP70 does not mediate survival in young septic hosts. In contrast, mortality was higher in aged HSP70−/− mice than aged WT mice subjected to CLP (p=0.01), suggesting HSP70 mediates mortality in sepsis in an age-dependent fashion. Compared to WT mice, aged septic HSP70−/− mice had increased gut epithelial apoptosis and pulmonary inflammation. In addition, HSP70−/−mice had increased systemic levels of TNF-α, IL-6, IL-10 and IL-1β compared to WT mice. These data demonstrate that HSP70 is a key determinant of mortality in aged but not young hosts in sepsis. HSP70 may play a protective role in an age-dependent response to sepsis by preventing excessive gut apoptosis and both pulmonary and systemic inflammation. PMID:21296977

  14. The role of heat shock protein 70 in mediating age-dependent mortality in sepsis.

    Science.gov (United States)

    McConnell, Kevin W; Fox, Amy C; Clark, Andrew T; Chang, Nai-Yuan Nicholas; Dominguez, Jessica A; Farris, Alton B; Buchman, Timothy G; Hunt, Clayton R; Coopersmith, Craig M

    2011-03-15

    Sepsis is primarily a disease of the aged, with increased incidence and mortality occurring in aged hosts. Heat shock protein (HSP) 70 plays an important role in both healthy aging and the stress response to injury. The purpose of this study was to determine the role of HSP70 in mediating mortality and the host inflammatory response in aged septic hosts. Sepsis was induced in both young (6- to 12-wk-old) and aged (16- to 17-mo-old) HSP70(-/-) and wild-type (WT) mice to determine whether HSP70 modulated outcome in an age-dependent fashion. Young HSP70(-/-) and WT mice subjected to cecal ligation and puncture, Pseudomonas aeruginosa pneumonia, or Streptococcus pneumoniae pneumonia had no differences in mortality, suggesting HSP70 does not mediate survival in young septic hosts. In contrast, mortality was higher in aged HSP70(-/-) mice than aged WT mice subjected to cecal ligation and puncture (p = 0.01), suggesting HSP70 mediates mortality in sepsis in an age-dependent fashion. Compared with WT mice, aged septic HSP70(-/-) mice had increased gut epithelial apoptosis and pulmonary inflammation. In addition, HSP70(-/-) mice had increased systemic levels of TNF-α, IL-6, IL-10, and IL-1β compared with WT mice. These data demonstrate that HSP70 is a key determinant of mortality in aged, but not young hosts in sepsis. HSP70 may play a protective role in an age-dependent response to sepsis by preventing excessive gut apoptosis and both pulmonary and systemic inflammation.

  15. EEVD motif of heat shock cognate protein 70 contributes to bacterial uptake by trophoblast giant cells

    Directory of Open Access Journals (Sweden)

    Kim Suk

    2009-12-01

    Full Text Available Abstract Background The uptake of abortion-inducing pathogens by trophoblast giant (TG cells is a key event in infectious abortion. However, little is known about phagocytic functions of TG cells against the pathogens. Here we show that heat shock cognate protein 70 (Hsc70 contributes to bacterial uptake by TG cells and the EEVD motif of Hsc70 plays an important role in this. Methods Brucella abortus and Listeria monocytogenes were used as the bacterial antigen in this study. Recombinant proteins containing tetratricopeptide repeat (TPR domains were constructed and confirmation of the binding capacity to Hsc70 was assessed by ELISA. The recombinant TPR proteins were used for investigation of the effect of TPR proteins on bacterial uptake by TG cells and on pregnancy in mice. Results The monoclonal antibody that inhibits bacterial uptake by TG cells reacted with the EEVD motif of Hsc70. Bacterial TPR proteins bound to the C-terminal of Hsc70 through its EEVD motif and this binding inhibited bacterial uptake by TG cells. Infectious abortion was also prevented by blocking the EEVD motif of Hsc70. Conclusions Our results demonstrate that surface located Hsc70 on TG cells mediates the uptake of pathogenic bacteria and proteins containing the TPR domain inhibit the function of Hsc70 by binding to its EEVD motif. These molecules may be useful in the development of methods for preventing infectious abortion.

  16. Preliminary X-ray crystallographic studies of yeast mitochondrial protein Tom70p

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Yunkun [Department of Cell Biology, Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham (United States); McCombs, Debbie; Nagy, Lisa; DeLucas, Lawrence [Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham (United States); Sha, Bingdong, E-mail: bdsha@uab.edu [Department of Cell Biology, Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham (United States)

    2006-03-01

    Tom70p is an important translocase of the outer membrane complex member and a major surface receptor of the protein-translocation machinery in the outer mitochondrial membrane. To investigate the mechanism by which Tom70p functions to deliver the mitochondrial protein precursors, the cytosolic fragment of yeast Tom70p (cTom70p) has been crystallized. Protein translocations across mitochondrial membranes play critical roles in mitochondrion biogenesis. Protein transport from the cell cytosol to the mitochondrial matrix is carried out by the translocase of the outer membrane (TOM) complex and the translocase of the inner membrane (TIM) complexes. Tom70p is an important TOM-complex member and a major surface receptor of the protein-translocation machinery in the outer mitochondrial membrane. To investigate the mechanism by which Tom70p functions to deliver the mitochondrial protein precursors, the cytosolic fragment of yeast Tom70p (cTom70p) was crystallized. The crystals diffract to 3.2 Å using a synchrotron X-ray source and belong to space group P2{sub 1}, with unit-cell parameters a = 44.89, b = 168.78, c = 83.41 Å, α = 90.00, β = 102.74, γ = 90.00°. There are two Tom70p molecules in one asymmetric unit, which corresponds to a solvent content of approximately 51%. Structure determination by MAD methods is under way.

  17. Efficient Regular Perovskite Solar Cells Based on Pristine [70]Fullerene as Electron-Selective Contact.

    Science.gov (United States)

    Collavini, Silvia; Kosta, Ivet; Völker, Sebastian F; Cabanero, German; Grande, Hans J; Tena-Zaera, Ramón; Delgado, Juan Luis

    2016-06-08

    [70]Fullerene is presented as an efficient alternative electron-selective contact (ESC) for regular-architecture perovskite solar cells (PSCs). A smart and simple, well-described solution processing protocol for the preparation of [70]- and [60]fullerene-based solar cells, namely the fullerene saturation approach (FSA), allowed us to obtain similar power conversion efficiencies for both fullerene materials (i.e., 10.4 and 11.4 % for [70]- and [60]fullerene-based devices, respectively). Importantly, despite the low electron mobility and significant visible-light absorption of [70]fullerene, the presented protocol allows the employment of [70]fullerene as an efficient ESC. The [70]fullerene film thickness and its solubility in the perovskite processing solutions are crucial parameters, which can be controlled by the use of this simple solution processing protocol. The damage to the [70]fullerene film through dissolution during the perovskite deposition is avoided through the saturation of the perovskite processing solution with [70]fullerene. Additionally, this fullerene-saturation strategy improves the performance of the perovskite film significantly and enhances the power conversion efficiency of solar cells based on different ESCs (i.e., [60]fullerene, [70]fullerene, and TiO2 ). Therefore, this universal solution processing protocol widens the opportunities for the further development of PSCs. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. Interspecific variation of intracellular localization and postirradiation movement of Ku70-protein in fibroblastic cells

    International Nuclear Information System (INIS)

    Endoh, Daiji; Hayashi, Masanobu; Okui, Toyo; Kawase, Shiro; Kon, Yasushiro

    2003-01-01

    Ku (Ku70 and Ku80) Proteins are known as components of DNA-dependent protein kinase (DNA-PK) and play an important role for DNA repair. We previously reported that more than 70% of Ku proteins were located in cytoplasm of rat cells, the Ku proteins moved into nuclei of normal rat cells after X-irradiation, Ku proteins also moved into nuclei after X-irradiation but were not retained in nucleus of radiosensitive LEC rat cells. While reports have been shown about mechanisms on nuclear localization of Ku proteins, how Ku proteins export from nucleus is poorly understood. Here we show that C-terminal region of Ku70 protein is important for its cytoplasmic localization. When transfected into LEC rat cells, exogenous intact Ku70 (1-609) tagged with enhanced green fluorescent protein (EGFP-Ku70) localized mainly in the cytoplasm, whereas C-terminal-deletion mutant of Ku70 (1-593) tagged with EGFP (EGFP-Ku70D) was mainly localized in the nucleus. After X-irradiation, the endogenous intact EGFP-Ku70 once moved into nucleus, but returned into the cytoplasm. On the other hand, EGFP-Ku70D was retained in nucleus for two hours after X-irradiation. These results suggest that C-terminal region of Ku70 is included in the postirradiation nuclear export. Next, we investigated the intracellular localization of Ku70 proteins and the movement after X-irradiation of fibroblastic cells prepared from some mammalian species. Ku70 proteins were localized in nucleus and the postirradiation-extranuclear transport was not observed in human and African green monkey cells. On the other hand, Ku70 proteins were mainly localized in cytoplasm and moved into nucleus in mouse, Chinese hamster, Golden hamster, cotton rat, squirrel, cat and dog cells. These results may show that alternatively Ku70 protein is localized in the cytoplasm or nucleus depends on species and translocation of cytoplasmic Ku70 into nucleus is a response against low dose irradiation in fibroblasts of rodents, cats and dogs

  19. Expression analysis of HSP70 in the testis of Octopus tankahkeei under thermal stress.

    Science.gov (United States)

    Long, Ling-Li; Han, Ying-Li; Sheng, Zhang; Du, Chen; Wang, You-Fa; Zhu, Jun-Quan

    2015-09-01

    The gene encoding heat shock protein 70 (HSP70) was identified in Octopus tankahkeei by homologous cloning and rapid amplification of cDNA ends (RACE). The full-length cDNA (2471 bp) consists of a 5'-untranslated region (UTR) (89 bp), a 3'-UTR (426 bp), and an open reading frame (1956 bp) that encodes 651 amino acid residues with a predicted molecular mass of 71.8 kDa and an isoelectric point of 5.34. Based on the amino acid sequence analysis and multiple sequence alignment, this cDNA is a member of cytoplasmic hsp70 subfamily of the hsp70 family and was designated as ot-hsp70. Tissue expression analysis showed that HSP70 expression is highest in the testes when all examined organs were compared. Immunohistochemistry analysis, together with hematoxylin-eosin staining, revealed that the HSP70 protein was expressed in all spermatogenic cells, but not in fibroblasts. In addition, O. tankahkeei were heat challenged by exposure to 32 °C seawater for 2 h, then returned to 13 °C for various recovery time (0-24 h). Relative expression of ot-hsp70 mRNA in the testes was measured at different time points post-challenge by quantitative real-time PCR. A clear time-dependent mRNA expression of ot-hsp70 after thermal stress indicates that the HSP70 gene is inducible. Ultrastructural changes of the heat-stressed testis were observed by transmission electron microscopy. We suggest that HSP70 plays an important role in spermatogenesis and testis protection against thermal stress in O. tankahkeei. Copyright © 2015 Elsevier Inc. All rights reserved.

  20. Stress-induced localization of HSPA6 (HSP70B') and HSPA1A (HSP70-1) proteins to centrioles in human neuronal cells.

    Science.gov (United States)

    Khalouei, Sam; Chow, Ari M; Brown, Ian R

    2014-05-01

    The localization of yellow fluorescent protein (YFP)-tagged HSP70 proteins was employed to identify stress-sensitive sites in human neurons following temperature elevation. Stable lines of human SH-SY5Y neuronal cells were established that expressed YFP-tagged protein products of the human inducible HSP70 genes HSPA6 (HSP70B') and HSPA1A (HSP70-1). Following a brief period of thermal stress, YFP-tagged HSPA6 and HSPA1A rapidly appeared at centrioles in the cytoplasm of human neuronal cells, with HSPA6 demonstrating a more prolonged signal compared to HSPA1A. Each centriole is composed of a distal end and a proximal end, the latter linking the centriole doublet. The YFP-tagged HSP70 proteins targeted the proximal end of centrioles (identified by γ-tubulin marker) rather than the distal end (centrin marker). Centrioles play key roles in cellular polarity and migration during neuronal differentiation. The proximal end of the centriole, which is involved in centriole stabilization, may be stress-sensitive in post-mitotic, differentiating human neurons.

  1. Ekspresi Human Leukocyte Antigen-G (HLA-G dan Heat-Shock Protein-70 (Hsp-70 pada Pertumbuhan Janin Terhambat

    Directory of Open Access Journals (Sweden)

    Sri Sulistyowati

    2014-03-01

    Full Text Available Intra uterine growth retardation (IUGR is one of the leading causes of higher morbidity and mortality in perinatal. Immune maladaptation affects trophoblast invasion and spiralis arteria remodeling that will cause placental tissue hypoxia. This research aimed to analyze human leukocyte antigen-G (HLA-G and heat-shock protein-70 (Hsp-70 expression on the IUGR trophoblast and normal pregnancy, by applying analytical observational method and cross sectional approach. This research was conducted at the Obstetric and Gynecology Department of Dr. Moewardi Hospital Surakarta from November to December 2011. The total samples were 30, divided into two groups. There were 15 samples trophoblast on IUGR and 15 samples trophoblast from normal pregnancy. All samples were tested for HLA-G and Hsp-70 using immunohistochemistry. The data were analyzed by using t-test. The mean of HLA-G expression on the IUGR groups was 32.42±8.90 and on the normal pregnancy groups was 43.92±14.91 (p=0.016. Heat-shock protein70 expression on the IUGR groups was 2.4355+0.26647 and on the normal pregnancy groups was 1.5920+0.17142 with p=0.008. In conclusion, in IUGR, the HLA-G expression is lower and the Hsp-70 expression is higher than in normal pregnancy.

  2. 41 CFR 301-70.803 - How must we authorize travel on a Government aircraft?

    Science.gov (United States)

    2010-07-01

    ... qualified non-crewmembers on a flight in which they are also traveling (i.e., being transported from point-to-point) are considered travelers and must be authorized to travel on Government aircraft according... travel on a Government aircraft? 301-70.803 Section 301-70.803 Public Contracts and Property Management...

  3. 27 CFR 70.444 - Importation of arms, ammunition, and implements of war.

    Science.gov (United States)

    2010-04-01

    ..., ammunition, and implements of war. 70.444 Section 70.444 Alcohol, Tobacco Products and Firearms ALCOHOL AND... implements of war. Part 47 of title 27 CFR implements Executive Order 11958 and supplements the import... implements of war, (b) Import permit requirements, (c) Import certification and verification, (d) Import...

  4. 41 CFR 102-73.70 - Are Executive agencies required to acquire leased space by negotiation?

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Are Executive agencies required to acquire leased space by negotiation? 102-73.70 Section 102-73.70 Public Contracts and Property Management Federal Property Management Regulations System (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 73-REAL ESTATE ACQUISITION...

  5. 48 CFR 2152.203-70 - Misleading, deceptive, or unfair advertising.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Misleading, deceptive, or unfair advertising. 2152.203-70 Section 2152.203-70 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CLAUSES AND FORMS PRECONTRACT PROVISIONS AND CONTRACT...

  6. 12 CFR 516.70 - Where must I publish the public notice?

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Where must I publish the public notice? 516.70 Section 516.70 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION... for permission to organize under § 543.2 of this chapter, a Bank Merger Act application under 563.22(a...

  7. 12 CFR 502.70 - How must I pay an application fee?

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How must I pay an application fee? 502.70 Section 502.70 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ASSESSMENTS AND... Thrift Supervision. You must include a statement of the fee and how you calculated the fee. ...

  8. 42 CFR 430.70 - Notice of hearing or opportunity for hearing.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Notice of hearing or opportunity for hearing. 430.70 Section 430.70 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL ASSISTANCE PROGRAMS GRANTS TO STATES FOR MEDICAL ASSISTANCE PROGRAMS Hearings on Conformity of State Medicaid Plans...

  9. 48 CFR 3052.209-70 - Prohibition on contracts with corporate expatriates.

    Science.gov (United States)

    2010-10-01

    ... have substantial business activities in the foreign country in which or under the law of which the... with corporate expatriates. 3052.209-70 Section 3052.209-70 Federal Acquisition Regulations System... contracts with corporate expatriates. As prescribed at (HSAR) 48 CFR 3009.104-75, insert the following...

  10. Conserved structure and expression of hsp70 paralogs in teleost fishes

    DEFF Research Database (Denmark)

    Metzger, David C.H.; Hansen, Jakob Hemmer; Schulte, Patricia M.

    2016-01-01

    present in the F. heteroclitus genome. Comparison of expression patterns in F. heteroclitus and Gasterosteus aculeatus demonstrates that hsp70-2 has a higher fold increase than hsp70-1 following heat shock in gill but not in muscle tissue, revealing a conserved difference in expression patterns between...

  11. 48 CFR 652.239-70 - Information Technology Security Plan and Accreditation.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Information Technology... Clauses 652.239-70 Information Technology Security Plan and Accreditation. As prescribed in 639.107-70(a), insert the following provision: Information Technology Security Plan and Accreditation (SEP 2007) All...

  12. 48 CFR 3052.204-70 - Security requirements for unclassified information technology resources.

    Science.gov (United States)

    2010-10-01

    ... unclassified information technology resources. 3052.204-70 Section 3052.204-70 Federal Acquisition Regulations... for unclassified information technology resources. As prescribed in (HSAR) 48 CFR 3004.470-3, insert a clause substantially the same as follows: Security Requirements for Unclassified Information Technology...

  13. 41 CFR 105-70.040 - Stays ordered by the Department of Justice.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Stays ordered by the Department of Justice. 105-70.040 Section 105-70.040 Public Contracts and Property Management Federal... Stays ordered by the Department of Justice. If at any time the Attorney General or an Assistant Attorney...

  14. 21 CFR 70.20 - Packaging requirements for straight colors (other than hair dyes).

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Packaging requirements for straight colors (other than hair dyes). 70.20 Section 70.20 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH... straight colors (other than hair dyes). Straight colors shall be packaged in containers which prevent...

  15. Evaluation of 39 medical implants at 7.0 T

    Science.gov (United States)

    Feng, David X; McCauley, Joseph P; Morgan–Curtis, Fea K; Salam, Redoan A; Pennell, David R; Loveless, Mary E

    2015-01-01

    Objective: With increased signal to noise ratios, 7.0-T MRI has the potential to contribute unique information regarding anatomy and pathophysiology of a disease. However, concerns for the safety of subjects with metallic medical implants have hindered advancement in this field. The purpose of the present research was to evaluate the MRI safety for 39 commonly used medical implants at 7.0 T. Methods: Selected metallic implants were tested for magnetic field interactions, radiofrequency-induced heating and artefacts using standardized testing techniques. Results: 5 of the 39 implants tested may be unsafe for subjects undergoing MRI at 7.0 T. Conclusion: Implants were deemed either “MR Conditional” or “MR Unsafe” for the 7.0-T MRI environment. Further research is needed to expand the existing database categorizing implants that are acceptable for patients referred for MRI examinations at 7.0 T. Advances in knowledge: Lack of MRI testing for common metallic medical implants limits the translational potential of 7.0-T MRI. For safety reasons, patients with metallic implants are not allowed to undergo a 7.0-T MRI scan, precluding part of the population that can benefit from the detailed resolution of ultra-high-field MRIs. This investigation provides necessary MRI testing of common medical implants at 7.0 T. PMID:26481696

  16. 48 CFR 1052.201-70 - Contracting Officer's Technical Representative (COTR) Designation and Authority.

    Science.gov (United States)

    2010-10-01

    ... in writing. The term “technical direction” includes, without limitation, direction to the contractor... the actions of the contractor's employees. (d) Technical direction may be oral or in writing. The COTR... Technical Representative (COTR) Designation and Authority. 1052.201-70 Section 1052.201-70 Federal...

  17. 48 CFR 853.236-70 - VA Form 10-6298, Architect-Engineer Fee Proposal.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false VA Form 10-6298, Architect-Engineer Fee Proposal. 853.236-70 Section 853.236-70 Federal Acquisition Regulations System DEPARTMENT OF...-Engineer Fee Proposal. VA Form 10-6298, Architect-Engineer Fee Proposal, shall be used as prescribed in 836...

  18. 48 CFR 752.245-70 - Government property-USAID reporting requirements.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Government property-USAID reporting requirements. 752.245-70 Section 752.245-70 Federal Acquisition Regulations System AGENCY FOR... recommendation, USAID contracts, except for those for commercial items, must contain the following preface and...

  19. 10 CFR 70.23a - Hearing required for uranium enrichment facility.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Hearing required for uranium enrichment facility. 70.23a... MATERIAL License Applications § 70.23a Hearing required for uranium enrichment facility. The Commission... license for construction and operation of a uranium enrichment facility. The Commission will publish...

  20. 7 CFR 70.14 - Squabs and domesticated game birds; eligibility.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Squabs and domesticated game birds; eligibility. 70.14... Products General § 70.14 Squabs and domesticated game birds; eligibility. Squabs and domesticated game birds (including, but not being limited to, quail, pheasants, and wild species of ducks and geese raised...

  1. 48 CFR 2852.211-70 - Brand-name or equal.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Brand-name or equal. 2852... Forms SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 2852.211-70 Brand-name or equal. As prescribed in 2811.104-70, insert the following clause: Brand-Name or Equal (JAN 1985...

  2. 48 CFR 2811.104-70 - Brand-name or equal description.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Brand-name or equal... 2811.104-70 Brand-name or equal description. When a brand-name or equal description is used, the clause set forth in 2852.211-70, Brand-name or Equal, shall be inserted into the solicitation. ...

  3. 48 CFR 206.303-70 - Acquisitions in support of operations in Iraq or Afghanistan.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Acquisitions in support of operations in Iraq or Afghanistan. 206.303-70 Section 206.303-70 Federal Acquisition Regulations System... Afghanistan. The justification and approval addressed in FAR 6.303 is not required for acquisitions conducted...

  4. Association of HSP70 and its co-chaperones with Alzheimer's disease

    NARCIS (Netherlands)

    L. Broer (Linda); M.A. Ikram (Arfan); M. Schuur (Maaike); A.L. DeStefano (Anita); J.C. Bis (Joshua); F. Liu (Fan); F. Rivadeneira Ramirez (Fernando); A.G. Uitterlinden (André); A. Beiser (Alexa); W.T. Longstreth Jr; A. Hofman (Albert); Y.S. Aulchenko (Yurii); S. Seshadri (Sudha); A.L. Fitzpatrick (Annette); B.A. Oostra (Ben); M.M.B. Breteler (Monique); P. Tikka-Kleemola (Päivi)

    2011-01-01

    textabstractThe heat shock protein (HSP) 70 family has been implicated in the pathology of Alzheimer's disease (AD). In this study, we examined common genetic variations in the 80 genes encoding HSP70 and its co-chaperones. We conducted a study in a series of 462 patients and 5238 unaffected

  5. 45 CFR 1180.70 - Guidelines and standards for museum conservation projects.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Guidelines and standards for museum conservation projects. 1180.70 Section 1180.70 Public Welfare Regulations Relating to Public Welfare (Continued..., as applied to art, history, natural history, science and technology, and living collections: (1...

  6. 48 CFR 3019.708-70 - Solicitation provision and contract clauses.

    Science.gov (United States)

    2010-10-01

    ... HOMELAND SECURITY, HOMELAND SECURITY ACQUISITION REGULATION (HSAR) SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS The Small Business Subcontracting Program 3019.708-70 Solicitation provision and contract clauses. (a) The contracting officer shall insert the clause at (HSAR) 48 CFR 3052.219-70, Small Business...

  7. Association of eNOS and HSP70 gene polymorphisms with glaucoma in Pakistani cohorts.

    NARCIS (Netherlands)

    Ayub, H.; Khan, M.I.; Micheal, S.; Akhtar, F.; Ajmal, M.; Shafique, S.; Ali, S.H.; Hollander, A.I. den; Ahmed, A.; Qamar, R.

    2010-01-01

    PURPOSE: To investigate the involvement of stress-regulating genes, endothelial nitric oxide synthase (eNOS) and heat shock protein 70 (HSP70) with primary open angle glaucoma (POAG) and primary closed angle glaucoma (PCAG). METHODS: POAG and PCAG patients recruited from different areas of Pakistan

  8. 48 CFR 215.404-70 - DD Form 1547, Record of Weighted Guidelines Method Application.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 1547, Record of... TYPES CONTRACTING BY NEGOTIATION Contract Pricing 215.404-70 DD Form 1547, Record of Weighted Guidelines Method Application. Follow the procedures at PGI 215.404-70 for use of DD Form 1547 whenever a structured...

  9. 48 CFR 253.215-70 - DD Form 1547, Record of Weighted Guidelines Application.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false DD Form 1547, Record of... Forms 253.215-70 DD Form 1547, Record of Weighted Guidelines Application. Follow the procedures at PGI 253.215-70 for completing DD Form 1547. [71 FR 69495, Dec. 1, 2006] ...

  10. 48 CFR 253.213-70 - Completion of DD Form 1155, Order for Supplies or Services.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Completion of DD Form 1155... Forms 253.213-70 Completion of DD Form 1155, Order for Supplies or Services. Follow the procedures at PGI 253.213-70 for completion of DD Form 1155. [71 FR 3413, Jan. 23, 2006] ...

  11. 48 CFR 3052.235-70 - Dissemination of information-educational institutions.

    Science.gov (United States)

    2010-10-01

    ...: Dissemination of Information—Educational Institutions (DEC 2003) (a) The Department of Homeland Security (DHS... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Dissemination of information-educational institutions. 3052.235-70 Section 3052.235-70 Federal Acquisition Regulations System...

  12. 10 CFR 51.70 - Draft environmental impact statement-general.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Draft environmental impact statement-general. 51.70... Implementing Section 102(2) Environmental Impact Statements § 51.70 Draft environmental impact statement—general. (a) The NRC staff will prepare a draft environmental impact statement as soon as practicable...

  13. 10 CFR 70.20a - General license to possess special nuclear material for transport.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false General license to possess special nuclear material for transport. 70.20a Section 70.20a Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) DOMESTIC LICENSING OF... transport. (a) A general license is issued to any person to possess formula quantities of strategic special...

  14. 41 CFR 303-70.301 - Are there any limitations on the baggage we may transport?

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 4 2010-07-01 2010-07-01 false Are there any limitations on the baggage we may transport? 303-70.301 Section 303-70.301 Public Contracts and Property... on the baggage we may transport? Yes. You may only transport government property and the employee's...

  15. 48 CFR 652.225-70 - Arab League Boycott of Israel.

    Science.gov (United States)

    2010-10-01

    ... Israel. 652.225-70 Section 652.225-70 Federal Acquisition Regulations System DEPARTMENT OF STATE CLAUSES... League Boycott of Israel. As prescribed in 625.7002(a), insert the following provision: Arab League Boycott of Israel (AUG 1999) (a) Definitions. As used in this provision: Foreign person means any person...

  16. 21 CFR 70.5 - General restrictions on use of color additives.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false General restrictions on use of color additives. 70... GENERAL COLOR ADDITIVES General Provisions § 70.5 General restrictions on use of color additives. (a) Color additives for use in the area of the eye. No listing or certification of a color additive shall be...

  17. 46 CFR 111.70-3 - Motor controllers and motor-control centers.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Motor controllers and motor-control centers. 111.70-3... ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Motor Circuits, Controllers, and Protection § 111.70-3 Motor controllers and motor-control centers. (a) General. The enclosure for each motor controller or motor-control...

  18. 48 CFR 1852.245-70 - Contractor requests for Government-owned equipment.

    Science.gov (United States)

    2010-10-01

    ... the equipment and the reasons why contractor-owned property cannot be used, citing the applicable FAR... 1419, DOD Industrial Plant Equipment Requisition, or equivalent format, for each item requested and (ii... Government-owned equipment. 1852.245-70 Section 1852.245-70 Federal Acquisition Regulations System NATIONAL...

  19. 48 CFR 2452.239-70 - Background investigations for sensitive automated systems/applications.

    Science.gov (United States)

    2010-10-01

    ... for sensitive automated systems/applications. 2452.239-70 Section 2452.239-70 Federal Acquisition... automated systems/applications. As prescribed in 2439.107(a), insert the following clause: Background Investigations for Sensitive Automated Systems/Applications (OCT 1999) (a) General. This contract involves work...

  20. Structural basis for activation of ZAP-70 by phosphorylation of the SH2-kinase linker.

    Science.gov (United States)

    Yan, Qingrong; Barros, Tiago; Visperas, Patrick R; Deindl, Sebastian; Kadlecek, Theresa A; Weiss, Arthur; Kuriyan, John

    2013-06-01

    Serial activation of the tyrosine kinases Lck and ZAP-70 initiates signaling downstream of the T cell receptor. We previously reported the structure of an autoinhibited ZAP-70 variant in which two regulatory tyrosine residues (315 and 319) in the SH2-kinase linker were replaced by phenylalanine. We now present a crystal structure of ZAP-70 in which Tyr 315 and Tyr 319 are not mutated, leading to the recognition of a five-residue sequence register error in the SH2-kinase linker of the original crystallographic model. The revised model identifies distinct roles for these two tyrosines. As seen in a recently reported structure of the related tyrosine kinase Syk, Tyr 315 of ZAP-70 is part of a hydrophobic interface between the regulatory apparatus and the kinase domain, and the integrity of this interface would be lost upon engagement of doubly phosphorylated peptides by the SH2 domains. Tyr 319 is not necessarily dislodged by SH2 engagement, which activates ZAP-70 only ∼5-fold in vitro. In contrast, phosphorylation by Lck activates ZAP-70 ∼100-fold. This difference is due to the ability of Tyr 319 to suppress ZAP-70 activity even when the SH2 domains are dislodged from the kinase domain, providing stringent control of ZAP-70 activity downstream of Lck.

  1. 21 CFR 173.70 - Chloromethylated aminated styrene-divinylbenzene resin.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Chloromethylated aminated styrene-divinylbenzene resin. 173.70 Section 173.70 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) SECONDARY DIRECT FOOD ADDITIVES PERMITTED IN FOOD FOR HUMAN CONSUMPTION Polymer...

  2. 45 CFR 234.70 - Protective payments for the aged, blind, or disabled.

    Science.gov (United States)

    2010-10-01

    ..., administrator or fiscal agent of a nursing home, or social care, medical or nonmedical institution, except for... disabled. 234.70 Section 234.70 Public Welfare Regulations Relating to Public Welfare OFFICE OF FAMILY ASSISTANCE (ASSISTANCE PROGRAMS), ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN...

  3. Photochemical reactivity of aqueous fullerene clusters: C{sub 60} versus C{sub 70}

    Energy Technology Data Exchange (ETDEWEB)

    Hou, Wen-Che, E-mail: whou@mail.ncku.edu.tw; Huang, Shih-Hong

    2017-01-15

    Highlights: • Aqueous C{sub 60} and C{sub 70} clusters (nC{sub 60} and nC{sub 70}) formed through direct mixing with water adopted a face-centered cubic crystal structure. • The AQYs of nC{sub 60} were greater than those of nC{sub 70}. • Both nC{sub 60} and nC{sub 70} lost considerable organic carbon contents (>80%) after ∼8 months of outdoor sunlight irradiation. • The intermediate photoproducts of nC{sub 60} and nC{sub 70} exhibited an increased content of oxygen-containing functionalities. - Abstract: Over the past few years, there has been a strong interest in exploring the potential impact of fullerenes in the environment. Despite that both C{sub 60} and C{sub 70} have been detected in environmental matrices, the research on the impact of higher fullerenes, such as C{sub 70,} has been largely missing. This study evaluated and compared the phototransformation of aqueous C{sub 60} and C{sub 70} clusters (nC{sub 60} and nC{sub 70}) and their {sup 1}O{sub 2} production under sunlight and lamp light irradiation (315 nm, 360 nm and 420 nm). The nC{sub 60} and nC{sub 70} samples formed by direct mixing with water adopted a face-centered cubic (FCC) crystal structure. The apparent quantum yields (AQYs) of fullerene phototransformed were relatively constant over the examined wavelengths, while {sup 1}O{sub 2} production AQYs decreased with increased wavelengths. The long-term fate studies with outdoor sunlight indicated that both nC{sub 60} and nC{sub 70} lost considerable organic carbon contents (>80%) in water after ∼8 months of irradiation and that the intermediate photoproducts of nC{sub 60} and nC{sub 70} exhibited a progressively increased level of oxygen-containing functionalities. Overall, the study indicates that nC{sub 70} can be photochemically removed under sunlight conditions and that the photoreactivity of nC{sub 60} based on AQYs is greater than that of nC{sub 70}.

  4. High mobility group protein DSP1 negatively regulates HSP70 transcription in Crassostrea hongkongensis

    Energy Technology Data Exchange (ETDEWEB)

    Miao, Zongyu; Xu, Delin; Cui, Miao; Zhang, Qizhong, E-mail: zhangqzdr@126.com

    2016-06-10

    HSP70 acts mostly as a molecular chaperone and plays important roles in facilitating the folding of nascent peptides as well as the refolding or degradation of the denatured proteins. Under stressed conditions, the expression level of HSP70 is upregulated significantly and rapidly, as is known to be achieved by various regulatory factors controlling the transcriptional level. In this study, a high mobility group protein DSP1 was identified by DNA-affinity purification from the nuclear extracts of Crassostrea hongkongensis using the ChHSP70 promoter as a bait. The specific interaction between the prokaryotically expressed ChDSP1 and the FITC-labeled ChHSP70 promoter was confirmed by EMSA analysis. ChDSP1 was shown to negatively regulate ChHSP70 promoter expression by Luciferase Reporter Assay in the heterologous HEK293T cells. Both ChHSP70 and ChDSP1 transcriptions were induced by either thermal or CdCl{sub 2} stress, while the accumulated expression peaks of ChDSP1 were always slightly delayed when compared with that of ChHSP70. This indicates that ChDSP1 is involved, very likely to exert its suppressive role, in the recovery of the ChHSP70 expression from the induced level to its original state. This study is the first to report negative regulator of HSP70 gene transcription, and provides novel insights into the mechanisms controlling heat shock protein expression. -- Highlights: •HMG protein ChDSP1 shows affinity to ChHSP70 promoter in Crassostrea hongkongensis. •ChDSP1 negatively regulates ChHSP70 transcription. •ChHSP70 and ChDSP1 transcriptions were coordinately induced by thermal/Cd stress. •ChDSP1 may contribute to the recovery of the induced ChHSP70 to its original state. •This is the first report regarding negative regulator of HSP70 transcription.

  5. Plasmodial Hsp70s are functionally adapted to the malaria parasite life cycle

    Directory of Open Access Journals (Sweden)

    Jude M Przyborski

    2015-06-01

    Full Text Available The human malaria parasite, Plasmodium falciparum, encodes a minimal complement of six heat shock protein 70s (PfHSP70s, some of which are highly expressed and are thought to play an important role in the survival and pathology of the parasite. In addition to canonical features of molecular chaperones, these HSP70s possess properties that reflect functional adaptation to a parasitic life style, including resistance to thermal insult during fever periods and host-parasite interactions. The parasite even exports an HSP70 to the host cell where it is likely to be involved in host cell modification. This review focuses on the features of the PfHSP70s, particularly with respect to their adaptation to the malaria parasite life cycle.

  6. Heat Loss Testing of Schott's 2008 PTR70 Parabolic Trough Receiver

    Energy Technology Data Exchange (ETDEWEB)

    Burkholder, Frank [National Renewable Energy Lab. (NREL), Golden, CO (United States); Kutscher, Chuck [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2009-05-01

    Two Schott 2008 model year PTR70 HCEs were tested on NREL's heat loss test stand from 100 - 500 deg C in 50 deg C increments. Absorber emittance was determined from the laboratory testing so that the performance of the HCEs could be modeled in a parabolic trough collector. Collector/HCE simulation results for many different field operation conditions were used to create heat loss correlationcoefficients for Excelergy and SAM. SAM estimates that the decreased emittance of the 2008 PTR70 will decrease the LCOE for parabolic trough power plants by 0.5 cents/kWh and increase the electricity generated by 5% relative to previous PTR70s. These conclusions assume that the 2008 PTR70 is supplied at the same cost and with the same optical performance as earlier PTR70 models.

  7. Hsp70 stabilizes lysosomes and reverts Niemann-Pick disease-associated lysosomal pathology

    DEFF Research Database (Denmark)

    Kirkegaard, Thomas; Roth, Anke G; Petersen, Nikolaj H T

    2010-01-01

    Heat shock protein 70 (Hsp70) is an evolutionarily highly conserved molecular chaperone that promotes the survival of stressed cells by inhibiting lysosomal membrane permeabilization, a hallmark of stress-induced cell death. Clues to its molecular mechanism of action may lay in the recently...... reported stress- and cancer-associated translocation of a small portion of Hsp70 to the lysosomal compartment. Here we show that Hsp70 stabilizes lysosomes by binding to an endolysosomal anionic phospholipid bis(monoacylglycero)phosphate (BMP), an essential co-factor for lysosomal sphingomyelin metabolism......-is also associated with a marked decrease in lysosomal stability, and this phenotype can be effectively corrected by treatment with recombinant Hsp70. Taken together, these data open exciting possibilities for the development of new treatments for lysosomal storage disorders and cancer with compounds...

  8. High Spatial Resolution Cardiovascular Magnetic Resonance at 7.0 Tesla in Patients with Hypertrophic Cardiomyopathy – First Experiences: Lesson Learned from 7.0 Tesla

    Science.gov (United States)

    Prothmann, Marcel; von Knobelsdorff-Brenkenhoff, Florian; Töpper, Agnieszka; Dieringer, Matthias A.; Shahid, Etham; Graessl, Andreas; Rieger, Jan; Lysiak, Darius; Thalhammer, C.; Huelnhagen, Till; Kellman, Peter; Niendorf, Thoralf; Schulz-Menger, Jeanette

    2016-01-01

    Background Cardiovascular Magnetic Resonance (CMR) provides valuable information in patients with hypertrophic cardiomyopathy (HCM) based on myocardial tissue differentiation and the detection of small morphological details. CMR at 7.0T improves spatial resolution versus today’s clinical protocols. This capability is as yet untapped in HCM patients. We aimed to examine the feasibility of CMR at 7.0T in HCM patients and to demonstrate its capability for the visualization of subtle morphological details. Methods We screened 131 patients with HCM. 13 patients (9 males, 56 ±31 years) and 13 healthy age- and gender-matched subjects (9 males, 55 ±31years) underwent CMR at 7.0T and 3.0T (Siemens, Erlangen, Germany). For the assessment of cardiac function and morphology, 2D CINE imaging was performed (voxel size at 7.0T: (1.4x1.4x2.5) mm3 and (1.4x1.4x4.0) mm3; at 3.0T: (1.8x1.8x6.0) mm3). Late gadolinium enhancement (LGE) was performed at 3.0T for detection of fibrosis. Results All scans were successful and evaluable. At 3.0T, quantification of the left ventricle (LV) showed similar results in short axis view vs. the biplane approach (LVEDV, LVESV, LVMASS, LVEF) (p = 0.286; p = 0.534; p = 0.155; p = 0.131). The LV-parameters obtained at 7.0T where in accordance with the 3.0T data (pLVEDV = 0.110; pLVESV = 0.091; pLVMASS = 0.131; pLVEF = 0.182). LGE was detectable in 12/13 (92%) of the HCM patients. High spatial resolution CINE imaging at 7.0T revealed hyperintense regions, identifying myocardial crypts in 7/13 (54%) of the HCM patients. All crypts were located in the LGE-positive regions. The crypts were not detectable at 3.0T using a clinical protocol. Conclusions CMR at 7.0T is feasible in patients with HCM. High spatial resolution gradient echo 2D CINE imaging at 7.0T allowed the detection of subtle morphological details in regions of extended hypertrophy and LGE. PMID:26863618

  9. FLZ Attenuates α-Synuclein-Induced Neurotoxicity by Activating Heat Shock Protein 70.

    Science.gov (United States)

    Bao, Xiu-Qi; Wang, Xiao-Liang; Zhang, Dan

    2017-01-01

    Parkinson's disease (PD) is the second most prevalent neurodegenerative disease. The pathology of PD is caused by progressive degeneration of dopaminergic neurons and is characterized by the presence of intracellular inclusions known as Lewy bodies, composed mainly of α-synuclein. Heat shock proteins (HSPs) are crucial in protein quality control in cells. HSP70 in particular prevents the aggregation of protein aggregation, such as α-synuclein, providing a degree of protection against PD. The compound FLZ has been shown to protect several PD models in previous studies and was reported as an HSP inducer to protect against MPP + -induced neurotoxicity, but the mechanism remains unclear. In this study, we investigated the effects of FLZ-mediated HSP70 induction in α-synuclein transgenic mice and cells. FLZ treatment alleviated motor dysfunction and improved dopaminergic neuronal function in α-synuclein transgenic mice. HSP70 protein expression and transcriptional activity were increased by FLZ treatment, eliciting a reduction of α-synuclein aggregation and associated toxicity. The inhibition of HSP70 by quercetin or HSP70 siRNA markedly attenuated the neuroprotective effects of FLZ, confirming that FLZ exerted a neuroprotective effect through HSP70. We revealed that FLZ directly bound to and increased the expression of Hip, a cochaperone of HSP70, which in turn enhanced HSP70 activity. In conclusion, we defined a critical role for HSP70 and its cochaperones activated by FLZ in preventing neurodegeneration and proposed that targeting the HSP70 system may represent a potential therapy for α-synuclein-related diseases, such as PD.

  10. The significance of anti-DFS70 antibodies in the diagnosis of autoimmune disorders

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    Barbara Nieradko-Iwanicka

    2017-06-01

    Full Text Available Background . Anti-DFS70 antibodies are a subgroup of antinuclear antibodies (ANA. They are connected with the dense fine speckled autoantigen of 70 kD, known as the lens epithelium-derived growth factor p75. Objectives. The objective of the review is to present the role of anti-DFS70 antibodies in the diagnosis of autoimmune diseases on the basis of recent publications. Material and methods . The authors searched for articles in the Pubmed database using the key words: anti-DFS70 antibodies, systemic autoimmune rheumatic diseases and autoimmune disorders. Results . Anti-DFS70 antibodies can be detected in eye diseases, atopic diseases, alopecia areata, fibromyalgia, asthma, chronic fatigue syndrome, tumors, Hashimoto’s disease, Graves’ disease, Behcet’s disease, inflammatory bowel diseases, neoplasms and in infectious diseases. These antibodies are sometimes detected in patients with ANA-associated rheumatic diseases (AARD . Up to 20% of serum samples from healthy individuals (HI are ANA-positive. This is probably due to the presence of monospecific anti-DFS70 antibodies. Monospecific anti-DFS70 antibodies are not associated with AARD , but mixed anti-DFS70 can be found in AARD . Conclusions . Family physicians usually do not order ANA, extractable nuclear antigens (ENA or anti-DFS70 antibodies, but they should be aware that anti-DFS70 antibodies are biomarkers that can discriminate AARD from non-AARD , save patients from unnecessary, potentially toxic treatment and save finances typically spent on retesting and visits to specialists.

  11. te70).

    African Journals Online (AJOL)

    Dunn (1975) recorded a similar response regarding the total LH response, but not the peak LH level, in heifers fed 6I% of their energy requirements. In an earlier study by Dunn, Rone. Kaltenbach, van der walt, Riley. & Akbar (1974) the peak LH was in effect higher in underfed beef cows. In the study reported here, on day.

  12. Specific Genetic Immunotherapy Induced by Recombinant Vaccine Alpha-Fetoprotein-Heat Shock Protein 70 Complex

    Science.gov (United States)

    Wang, Xiaoping; Lin, Huanping; Wang, Qiaoxia

    Purposes: To construct a recombinant vaccine alpha-fetoprotein (AFP)-heat shock protein (HSP70) complex, and study its ability to induce specific CTL response and its protective effect against AFP-producing tumor. Material/Methods: A recombinant vaccine was constructed by conjugating mouse alpha-fetoprotein to heat shock protein 70. By way of intracutaneous injection, mice were primed and boosted with recombinant vaccine mAFP/HSP70, whereas single mAFP or HSP70 injection as controls. The ELISPOT and ELISA were used to measure the frequency of cells producing the cytokine IFN-γ in splenocytes and the level of anti-AFP antibody of serum from immunized mice respectively. In vivo tumor challenge were carried out to assess the immune effect of the recombinant vaccine. Results: By recombinant mAFP/HSP70 vaccine immunization, the results of ELISPOT and ELISA showed that the number of splenic cells producing IFN-γ and the level of anti-AFP antibody of serum were significantly higher in mAFP/HSP70 group than those in mAFP and HSP70 groups (108.50±11.70 IFN-γ spots/106 cells vs 41.60±10.40 IFN-γ spots/106 cells, 7.32±3.14 IFN-γ spots/106 cells, P<0.01; 156.32±10.42 μg/mL vs 66.52±7.35 μg/mL, 5.73±2.89 μg/mL, P<0.01). The tumor volume in mAFP/HSP70 group was significantly smaller than that in mAFP and HSP70 groups (42.44±7.14 mm3 vs 392.23±12.46 mm3, 838.63±13.84 mm3, P<0.01). Conclusions: The study further confirmed the function of heat shock protein 70's immune adjuvant. Sequential immunization with recombinant mAFP/HSP70 vaccine could generate effective antitumor immunity on AFP-producing tumor. The recombined mAFP/HSP70 vaccine may be suitable for serving as an immunotherapy for hepatocellular carcinoma.

  13. Ku70 is required for late B cell development and immunoglobulin heavy chain class switching.

    Science.gov (United States)

    Manis, J P; Gu, Y; Lansford, R; Sonoda, E; Ferrini, R; Davidson, L; Rajewsky, K; Alt, F W

    1998-06-15

    Immunoglobulin (Ig) heavy chain (HC) class switch recombination (CSR) is a late B cell process that involves intrachromosomal DNA rearrangement. Ku70 and Ku80 form a DNA end-binding complex required for DNA double strand break repair and V(D)J recombination. Ku70(-/-) (K70T) mice, like recombination activating gene (RAG)-1- or RAG-2-deficient (R1T or R2T) mice, have impaired B and T cell development at an early progenitor stage, which is thought to result at least in part from defective V(D)J recombination (Gu, Y., K.J. Seidl, G.A. Rathbun, C. Zhu, J.P. Manis, N. van der Stoep, L. Davidson, H.L. Cheng, J.M. Sekiguchi, K. Frank, et al. 1997. Immunity. 7:653-665; Ouyang, H., A. Nussenzweig, A. Kurimasa, V.C. Soares, X. Li, C. Cordon-Cardo, W. Li, N. Cheong, M. Nussenzweig, G. Iliakis, et al. 1997. J. Exp. Med. 186:921-929). Therefore, to examine the potential role of Ku70 in CSR, we generated K70T mice that carry a germline Ig HC locus in which the JH region was replaced with a functionally rearranged VH(D)JH and Ig lambda light chain transgene (referred to as K70T/HL mice). Previously, we have shown that B cells from R1T or R2T mice carrying these rearranged Ig genes (R1T/HL or R2T/HL mice) can undergo CSR to IgG isotypes (Lansford, R., J. Manis, E. Sonoda, K. Rajewsky, and F. Alt. 1998. Int. Immunol. 10:325-332). K70T/HL mice had significant numbers of peripheral surface IgM+ B cells, which generated serum IgM levels similar to those of R2T/HL mice. However, in contrast to R2T/HL mice, K70T/HL mice had no detectable serum IgG isotypes. In vitro culture of K70T/HL B cells with agents that induce CSR in normal or R2T/HL B cells did lead to the induction of germline CH transcripts, indicating that initial signaling pathways for CSR were intact in K70T/HL cells. However, treatment with such agents did not lead to detectable CSR by K70T/HL B cells, and instead, led to cell death within 72 h. We conclude that Ku70 is required for the generation of B cells that have

  14. De novo characterisation of the greenlip abalone transcriptome (Haliotis laevigata) with a focus on the heat shock protein 70 (HSP70) family.

    Science.gov (United States)

    Shiel, Brett P; Hall, Nathan E; Cooke, Ira R; Robinson, Nicholas A; Strugnell, Jan M

    2015-02-01

    Abalone (Haliotis) are economically important molluscs for fisheries and aquaculture industries worldwide. Despite this, genomic resources for abalone and molluscs are still limited. Here we present a description and functional annotation of the greenlip abalone (Haliotis laevigata) transcriptome. We present a focused analysis on the heat shock protein 70 (HSP70) family of genes with putative functions affecting temperature stress and immunity. A total of ~38 million paired end Illumina reads were obtained, resulting in a Trinity assembly of 222,172 contigs with minimum length of 200 base pairs and maximum length of 33 kilobases. The 20,702 contigs were annotated with gene descriptions by BLAST. We created a program to maximise the number of functionally annotated genes, and over 10,000 contigs were assigned Gene ontologies (GO terms). By using CateGOrizer, immunity related GO terms for stressors such as heat, hypoxia, oxidative stress and wounding received the highest counts. Twenty-six contigs with homology to the HSP70 family of genes were identified. Ninety-one putative single-nucleotide polymorphisms were observed in the abalone HSP70 contigs. Eleven of these were considered non-synonymous. The annotated transcriptome described in this study will be a useful basis for future work investigating the genetic response of abalone to stress.

  15. Downregulated Ku70 and ATM associated to poor prognosis in colorectal cancer among Chinese patients

    Directory of Open Access Journals (Sweden)

    Lu YF

    2014-10-01

    Full Text Available Yuanfang Lu,1,2 Jingyan Gao,1,3 Yuanming Lu,1 1Department of Toxicology, School of Public Health, Guilin Medical University, Guangxi, People's Republic of China; 2Department of Clinical Research Center, Affiliated 2nd Hospital of Nanjing Medical University, Nanjing, People's Republic of China; 3Department of Human Anatomy and Histo-Embryology, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China Background: Double-strand DNA breaks (DSBs are a key factor in carcinogenesis. The necessary repair of DSBs is pivotal in maintaining normal cell division. To address the relationship between altered expression of DSB repair of proteins Ku70 and ataxia-telangiectasia mutated (ATM in colorectal cancer (CRC, we examined the expression levels and patterns of Ku70 and ATM in CRC samples. Methods: Expression and coexpression of Ku70 and ATM were investigated by using real-time quantitative polymerase chain reaction assays and confirmed further with fluorescent immunohistochemistry in CRC and pericancerous samples from 112 Chinese patients. Results: Downexpression patterns for both Ku70 and ATM were found in the CRC samples and were significantly associated with advanced tumor node metastasis stage and decreased 5-year overall survival rate. Conclusion: Downregulated Ku70 and ATM were associated with poor disease-free survival. Loss of Ku70 and ATM expression might act as a biomarker to predict poor prognosis in patients with CRC. Keywords: DNA double-strand breaks, ataxia-telangiectasia mutated, Ku70, colorectal cancer

  16. Expression and subcellular localization of p70S6 kinase under heart failure

    Directory of Open Access Journals (Sweden)

    Usenko V. S.

    2010-11-01

    Full Text Available The PI3K/PDK/Akt/mTOR/p70S6K signaling pathway is primary associated with the activation of insulin receptors and is important for cardiomyocytes survival. p70S6K is a key regulator of the speed and efficiency of protein biosynthesis within the cell. Recently the pro-apoptotic protein BAD has been identified as a new target for р70S6K1. BAD is inactivated in normal cardiomyocytes by р70S6K1 phosphorylation which prevents the cardiomyocytes apoptosis. Aim. To study possible changes in р70S6K1 expression and/or cellular localization at heart failure progression – in DCM- affected human myocardia and murine hearts with experimental DCM-like pathology. Methods. Western-blot analysis and immunohystochemistry. Results. The substantial decrease in р70S6K1 level was observed at the final stage of pathology progression and in the dynamics of DCM pathogenesis as well. For the first time relocalization of the protein to the connective tissue was shown according to the Western-blot results. Conclusions. The data obtained allow us to understand a possible role of р70S6K1 in the regulation of stress-induced apoptotic signaling in cardiomyocytes.

  17. Hsp70 in the atrial neuroendocrine units of the snail, Achatina fulica.

    Science.gov (United States)

    Martynova, M G; Bystrova, O A; Shabelnikov, S V; Margulis, B A; Prokofjeva, D S

    2007-04-01

    Heat shock proteins (Hsps) are evolutionary conserved peptides well known as molecular chaperones and stress proteins. Elevated levels of extracellular Hsps in blood plasma have been observed during the stress responses and some diseases. Information on the cellular sources of extracellular Hsps and mechanisms regulating their release is still scanty. Here we showed the presence and localization of Hsp70 in the neuroendocrine system in the atrium of the snail, Achatina fulica. The occurrence of the peptide in snail atrium lysate was detected by Western blot analysis. Immunoperoxidase and immunogold staining demonstrated that Hsp70-immunoreactivity is mainly confined to the peculiar atrial neuroendocrine units which are formed by nerve fibers tightly contacted with large granular cells. Immunolabelling intensity differed in morphologically distinct types of secretory granules in the granular cells. The pictures of exocytosis of Hsp70-immunolabeled granules from the granular cells were observed. In nerve bundles, axon profiles with Hsp70-immunoreactive and those with non-immunoreactive neurosecretory granules were found. In addition, Hsp70-like material was also revealed in the granules of glia-interstitial cells that accompanied nerve fibers. Our findings provide an immuno-morphological basis for a role of Hsp70 in the functioning of the neuroendocrine system in the snail heart, and show that the atrial granular cells are a probable source of extracellular Hsp70 in the snail hemolymph.

  18. High-temperature mechanical properties of high-purity 70 mass% Cr-Fe alloy

    Energy Technology Data Exchange (ETDEWEB)

    Asahina, M.; Harima, N.; Takaki, S.; Abiko, K. [Tohoku Univ., Sendai (Japan). Inst. for Materials Research

    2002-01-16

    An ingot of high-purity 70 mass% Cr-Fe alloy was prepared by high-frequency induction melting in a high-purity argon atmosphere using a cold copper crucible. Its tensile properties such as hot-ductility and tensile strength were measured, and compared with the results for a high-purity 50 mass% Cr-Fe alloy, a high-purity 60 mass% Cr-Fe alloy and a Ni-based super-alloy. The formation of {sigma}-phase was also examined. The purity of a 70Cr-Fe alloy (70 mass% Cr-Fe alloy) ingot is more than 99.98 mass% and the total amount of gaseous impurities (C, N, O, S, H) in the 70Cr-Fe alloy is 69.9 mass ppm. The strength of the 70Cr-Fe alloy is higher than those of the 60Cr-Fe alloy and the 50Cr-Fe alloy at the temperatures between 293 and 1573 K, without decrease in ductility with increasing Cr content. The 70Cr-Fe alloy also possesses excellent high-temperature ductility. The {sigma}-phase was not observed after aging of 3.6 Ms at 873 K. Consequently, the 70Cr-Fe alloy is an excellent alloy as the base of super heat-resistant alloys. (orig.)

  19. A comparison of piezosurgery and conventional surgery by heat shock protein 70 expression.

    Science.gov (United States)

    Gülnahar, Y; Hüseyin Köşger, H; Tutar, Y

    2013-04-01

    The effects of mechanical instruments on the viability of cells are essential in terms of regeneration. There is considerable interest in cell repair following damage mediated by dental surgical procedures. Cells can tolerate stress by expressing heat shock protein 70 (Hsp70). During and after surgical tooth removal, oxidative stress can activate Hsp70 expression proportional to the intensity of the stress signal stimulus to cope with stress. This study examined the expression of Hsp70 as a potential biomarker of immediate postoperative stress in patients undergoing two different surgical procedures of different severity. Expression of Hsp70 both at mRNA and at protein level in the conventional group was two-fold higher than that of the piezo group. This suggests that tooth movement by the piezo method causes relatively lower stress in the alveolar bone. Piezosurgery provides relatively low stress to the patients and this may help cell repair after the surgical procedure. Patients undergoing more aggressive surgery using conventional methods showed a significant increase in Hsp70 in the immediate postoperative period. Therefore, Hsp70 induction can be a potential tool as a prognostic surgical marker. Copyright © 2012 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

  20. Detection of 70 kDa heat shock protein in the saliva of dairy cows.

    Science.gov (United States)

    Lamy, Elsa; Jurkovich, Viktor; Rodrigues, Lénia; Geraldo, Ana; Cachucho, Liliana; Silva, Flávio; Matos, Catarina; Capela E Silva, Fernando; Pinheiro, Cristina; Könyves, László; Bakony, Mikolt; Pereira, Alfredo

    2017-08-01

    This Research Communication describes, for the first time, the detection of HSP70 in saliva of dairy cows. Thermal stress is a major environmental stress that limits animal growth, metabolism, and productivity. The cellular response to heat stress involves the synthesis of heat shock proteins (HSPs), presumably to protect the functional stability of cells at increasing temperatures. HSP70 has been found to be present in cattle blood serum and may also be present in other secretory fluids, such as saliva, as already observed in humans. The aim of this study was to detect heat shock protein HSP70 in bovine saliva. Saliva samples were taken from higher- (n = 5) and lower milk producing (n = 5) Holstein-Friesian cows in summer and in winter for the detection of HSP70. HSP70 concentrations were assayed using the ELISA technique. Salivary HSP70 concentrations ranged from 0·524 to 12·174 ng/ml in cows. Higher salivary HSP70 concentrations were significantly associated with higher milk production and higher environmental temperature, but not with rectal temperature.

  1. Feasibility of high-resolution pituitary MRI at 7.0 tesla

    International Nuclear Information System (INIS)

    Rotte, Alexandra A.J. de; Kolk, Anja G. van der; Rutgers, Dik; Luijten, Peter R.; Hendrikse, Jeroen; Zelissen, Pierre M.J.; Visser, Fredy

    2014-01-01

    Since the pituitary gland measures 3-8 mm, imaging with the highest possible spatial resolution is important for the detection of even smaller lesions such as those seen in Cushing's disease. In the current feasibility study, we tested a multi-sequence MRI protocol to visualize the pituitary gland in high resolution at 7.0 Tesla (7.0 T). Ten healthy volunteers were examined with a 7.0 T pituitary gland protocol. The protocol consisted of a T1-weighted magnetization-prepared inversion recovery (MPIR) turbo spin-echo (TSE) sequence and a T2-weighted TSE sequence. Additionally, this protocol was tested in five patients with clinical and biochemical suspicion of a microadenoma. The dedicated protocol was successful in visualizing normal pituitary anatomy. At 7.0 T compared to 1.5 T, four times as many slices covered the pituitary gland in sagittal and coronal direction. In three patients, a lesion was diagnosed at 7.0 T, and was confirmed by histopathology to be a microadenoma. Head-to-head comparisons of 7.0 T with 1.5 T and 3.0 T are needed with larger samples of patients and with imaging times feasible for clinical settings. However, the current study suggests that high-resolution 7.0 T MRI of the pituitary gland may provide new perspectives when used as a second-line diagnostic examination in the specific context of Cushing's disease. (orig.)

  2. Feasibility of high-resolution pituitary MRI at 7.0 tesla

    Energy Technology Data Exchange (ETDEWEB)

    Rotte, Alexandra A.J. de; Kolk, Anja G. van der; Rutgers, Dik; Luijten, Peter R.; Hendrikse, Jeroen [University Medical Center Utrecht, Department of Radiology, Heidelberglaan 100, Postbox 85500, Utrecht (Netherlands); Zelissen, Pierre M.J. [University Medical Center Utrecht, Department of Internal Medicine (Section of Endocrinology), Utrecht (Netherlands); Visser, Fredy [University Medical Center Utrecht, Department of Radiology, Heidelberglaan 100, Postbox 85500, Utrecht (Netherlands); Philips Healthcare, Best (Netherlands)

    2014-08-15

    Since the pituitary gland measures 3-8 mm, imaging with the highest possible spatial resolution is important for the detection of even smaller lesions such as those seen in Cushing's disease. In the current feasibility study, we tested a multi-sequence MRI protocol to visualize the pituitary gland in high resolution at 7.0 Tesla (7.0 T). Ten healthy volunteers were examined with a 7.0 T pituitary gland protocol. The protocol consisted of a T1-weighted magnetization-prepared inversion recovery (MPIR) turbo spin-echo (TSE) sequence and a T2-weighted TSE sequence. Additionally, this protocol was tested in five patients with clinical and biochemical suspicion of a microadenoma. The dedicated protocol was successful in visualizing normal pituitary anatomy. At 7.0 T compared to 1.5 T, four times as many slices covered the pituitary gland in sagittal and coronal direction. In three patients, a lesion was diagnosed at 7.0 T, and was confirmed by histopathology to be a microadenoma. Head-to-head comparisons of 7.0 T with 1.5 T and 3.0 T are needed with larger samples of patients and with imaging times feasible for clinical settings. However, the current study suggests that high-resolution 7.0 T MRI of the pituitary gland may provide new perspectives when used as a second-line diagnostic examination in the specific context of Cushing's disease. (orig.)

  3. Hsp70-Bag3 interactions regulate cancer-related signaling networks.

    Science.gov (United States)

    Colvin, Teresa A; Gabai, Vladimir L; Gong, Jianlin; Calderwood, Stuart K; Li, Hu; Gummuluru, Suryaram; Matchuk, Olga N; Smirnova, Svetlana G; Orlova, Nina V; Zamulaeva, Irina A; Garcia-Marcos, Mikel; Li, Xiaokai; Young, Z T; Rauch, Jennifer N; Gestwicki, Jason E; Takayama, Shinichi; Sherman, Michael Y

    2014-09-01

    Bag3, a nucleotide exchange factor of the heat shock protein Hsp70, has been implicated in cell signaling. Here, we report that Bag3 interacts with the SH3 domain of Src, thereby mediating the effects of Hsp70 on Src signaling. Using several complementary approaches, we established that the Hsp70-Bag3 module is a broad-acting regulator of cancer cell signaling by modulating the activity of the transcription factors NF-κB, FoxM1, Hif1α, the translation regulator HuR, and the cell-cycle regulators p21 and survivin. We also identified a small-molecule inhibitor, YM-1, that disrupts the Hsp70-Bag3 interaction. YM-1 mirrored the effects of Hsp70 depletion on these signaling pathways, and in vivo administration of this drug was sufficient to suppress tumor growth in mice. Overall, our results defined Bag3 as a critical factor in Hsp70-modulated signaling and offered a preclinical proof-of-concept that the Hsp70-Bag3 complex may offer an appealing anticancer target. ©2014 American Association for Cancer Research.

  4. Efficient gene replacements in ku70 disruption strain of Aspergillus chevalieri var. intermedius

    Directory of Open Access Journals (Sweden)

    Qingqing Huang

    2017-01-01

    Full Text Available Aspergillus chevalieri var. intermedius is a dominant filamentous fungal species in Fuzhuan tea and is associated with the quality and health benefits of this tea. The sexual or asexual reproduction of this fungus depends on the osmotic pressure of the tea. Efforts to enhance the beneficial effects of A. chevalieri var. intermedius are hampered by difficulties in disrupting its genes. To address this issue, we identified the A. chevalieri var. intermedius homolog (Acku70 of human Ku70 and generated an Acku70 disruption strain (ΔAcku70, aiming to improve the gene replacement efficiency. ΔAcku70 grew at a slightly lower rate in vitro than the wild-type strain; however, the two strains exhibited similar sensitivity to temperature, osmotic pressure and the effects of ethyl methane sulphonate and H2O2. The replacement efficiency of veA and flbA dramatically increased in ΔAcku70 compared to that in the wild type. The efficiency of flbA replacement increased from 2.6% to 80%, whereas the frequency of veA disruption increased from 15.2% to 83.9% and from 30.8% to 86.8%. Thus, ΔAcku70 is suitable for use as a type strain for large-scale functional genomic analysis of A. chevalieri var. intermedius.

  5. Broadening the functionality of a J-protein/Hsp70 molecular chaperone system.

    Science.gov (United States)

    Schilke, Brenda A; Ciesielski, Szymon J; Ziegelhoffer, Thomas; Kamiya, Erina; Tonelli, Marco; Lee, Woonghee; Cornilescu, Gabriel; Hines, Justin K; Markley, John L; Craig, Elizabeth A

    2017-10-01

    By binding to a multitude of polypeptide substrates, Hsp70-based molecular chaperone systems perform a range of cellular functions. All J-protein co-chaperones play the essential role, via action of their J-domains, of stimulating the ATPase activity of Hsp70, thereby stabilizing its interaction with substrate. In addition, J-proteins drive the functional diversity of Hsp70 chaperone systems through action of regions outside their J-domains. Targeting to specific locations within a cellular compartment and binding of specific substrates for delivery to Hsp70 have been identified as modes of J-protein specialization. To better understand J-protein specialization, we concentrated on Saccharomyces cerevisiae SIS1, which encodes an essential J-protein of the cytosol/nucleus. We selected suppressors that allowed cells lacking SIS1 to form colonies. Substitutions changing single residues in Ydj1, a J-protein, which, like Sis1, partners with Hsp70 Ssa1, were isolated. These gain-of-function substitutions were located at the end of the J-domain, suggesting that suppression was connected to interaction with its partner Hsp70, rather than substrate binding or subcellular localization. Reasoning that, if YDJ1 suppressors affect Ssa1 function, substitutions in Hsp70 itself might also be able to overcome the cellular requirement for Sis1, we carried out a selection for SSA1 suppressor mutations. Suppressing substitutions were isolated that altered sites in Ssa1 affecting the cycle of substrate interaction. Together, our results point to a third, additional means by which J-proteins can drive Hsp70's ability to function in a wide range of cellular processes-modulating the Hsp70-substrate interaction cycle.

  6. Hsp70/J-protein machinery from Glossina morsitans morsitans, vector of African trypanosomiasis.

    Directory of Open Access Journals (Sweden)

    Stephen J Bentley

    Full Text Available Tsetse flies (Glossina spp. are the sole vectors of the protozoan parasites of the genus Trypanosoma, the causative agents of African Trypanosomiasis. Species of Glossina differ in vector competence and Glossina morsitans morsitans is associated with transmission of Trypanosoma brucei rhodesiense, which causes an acute and often fatal form of African Trypanosomiasis. Heat shock proteins are evolutionarily conserved proteins that play critical roles in proteostasis. The activity of heat shock protein 70 (Hsp70 is regulated by interactions with its J-protein (Hsp40 co-chaperones. Inhibition of these interactions are emerging as potential therapeutic targets. The assembly and annotation of the G. m. morsitans genome provided a platform to identify and characterize the Hsp70s and J-proteins, and carry out an evolutionary comparison to its well-studied eukaryotic counterparts, Drosophila melanogaster and Homo sapiens, as well as Stomoxys calcitrans, a comparator species. In our study, we identified 9 putative Hsp70 proteins and 37 putative J-proteins in G. m. morsitans. Phylogenetic analyses revealed three evolutionarily distinct groups of Hsp70s, with a closer relationship to orthologues from its blood-feeding dipteran relative Stomoxys calcitrans. G. m. morsitans also lacked the high number of heat inducible Hsp70s found in D. melanogaster. The potential localisations, functions, domain organisations and Hsp70/J-protein partnerships were also identified. A greater understanding of the heat shock 70 (Hsp70 and J-protein (Hsp40 families in G. m. morsitans could enhance our understanding of the cell biology of the tsetse fly.

  7. Assessment of Analytic Water hammer Pressure Model of FAI/08-70

    International Nuclear Information System (INIS)

    Park, Ju Yeop; Yoo, Seung Hun; Seul, Kwang-Won

    2016-01-01

    In evaluating water hammer effect on the safety related systems, methods developed by the US utility are likely to be adopted in Korea. For example, the US utility developed specific methods to evaluate pressure and loading transient on piping due to water hammer as in FAI/08-70. The methods of FAI/08-70 would be applied in Korea when any regulatory request on the evaluation of water hammer effect due to the non-condensable gas accumulation in the safety related systems. Specifically, FAI/08-70 gives an analytic model which can be used to analyze the maximum transient pressure and maximum transient loading on the piping of the safety-related systems due to the non-condensable induced water hammer effect. Therefore, it seems to be meaningful to review the FAI/08-70 methods and attempt to apply the methods to a specific case to see if they really give reasonable estimate before the application of FAI/08-70 methods to domestic nuclear power plants. In the present study, analytic water hammer pressure model of FAI/08-70 is reviewed in detail and the model is applied to the specific experiment of FAI/08-70 to see if the analytic water hammer pressure model really gives reasonable estimate of the peak water hammer pressure. Specifically, we assess the experiment 52A of FAI/08-70 which adopts flushed initial condition with a short rising piping length and a high level piping length of 51inch. The calculated analytic water hammer pressure peak shows a close agreement with the measured experimental data of 52A. Unfortunately, however, the theoretical value is a little bit less than that of the experimental value. This implies the analytic model of FAI/08-70 is not conservative

  8. Mitochondrial carrier protein biogenesis: role of the chaperones Hsc70 and Hsp90.

    Science.gov (United States)

    Zara, Vincenzo; Ferramosca, Alessandra; Robitaille-Foucher, Philippe; Palmieri, Ferdinando; Young, Jason C

    2009-04-15

    Metabolite carrier proteins of the mitochondrial inner membrane share homology in their transmembrane domains, which also carries their targeting information. In addition, some carriers have cleavable presequences which are not essential for targeting, but have some other function before import. The cytosolic chaperones Hsc70 (heat-shock cognate 70) and Hsp90 (heat-shock protein 90) complex with carrier precursors and interact specifically with the Tom (translocase of the mitochondrial outer membrane) 70 import receptor to promote import. We analysed how the presequences of the PiC (phosphate carrier) and CIC (citrate carrier) relate to the mechanisms of chaperone-mediated import. Deletion of the PiC presequence reduced the efficiency of import but, notably, not by causing aggregation. Instead, binding of the protein to Hsc70 was reduced, as well as the dependence on Hsc70 for import. Hsp90 binding and function in import was not greatly affected, but it could not entirely compensate for the lack of Hsc70 interaction. Deletion of the presequence from CIC was shown to cause its aggregation, but had little effect on the contribution to import of either Hsc70 or Hsp90. The presequence of PiC, but not that of CIC, conferred Hsc70 binding to dihydrofolate reductase fusion proteins. In comparison, OGC (oxoglutarate carrier) lacks a presequence and was more soluble, though it is still dependent on both Hsc70 and Hsp90. We propose that carrier presequences evolved to improve targeting competence by different mechanisms, depending on physical properties of the precursors in the cytosolic targeting environment.

  9. 14-3-3σ induces heat shock protein 70 expression in hepatocellular carcinoma

    International Nuclear Information System (INIS)

    Liu, Chia-Chia; Wang, John; Shyue, Song-Kun; Sung, Li-Ying; Liou, Jun-Yang; Jan, Yee-Jee; Ko, Bor-Sheng; Wu, Yao-Ming; Liang, Shu-Man; Chen, Shyh-Chang; Lee, Yen-Ming; Liu, Tzu-An; Chang, Tzu-Ching

    2014-01-01

    14-3-3σ is implicated in promoting tumor development of various malignancies. However, the clinical relevance of 14-3-3σ in hepatocellular carcinoma (HCC) tumor progression and modulation and pathway elucidation remain unclear. We investigated 14-3-3σ expression in 109 HCC tissues by immunohistochemistry. Overexpression and knockdown experiments were performed by transfection with cDNA or siRNA. Protein expression and cell migration were determined by Western blot and Boyden chamber assay. In this study, we found that 14-3-3σ is abundantly expressed in HCC tumors. Stable or transient overexpression of 14-3-3σ induces the expression of heat shock factor-1α (HSF-1α) and heat shock protein 70 (HSP70) in HCC cells. Moreover, expression of 14-3-3σ significantly correlates with HSF-1α/HSP70 in HCC tumors and both 14-3-3σ and HSP70 overexpression are associated with micro-vascular thrombi in HCC patients, suggesting that 14-3-3σ/HSP70 expression is potentially involved in cell migration/invasion. Results of an in vitro migration assay indicate that 14-3-3σ promotes cell migration and that 14-3-3σ-induced cell migration is impaired by siRNA knockdown of HSP70. Finally, 14-3-3σ-induced HSF-1α/HSP70 expression is abolished by the knockdown of β-catenin or activation of GSK-3β. Our findings indicate that 14-3-3σ participates in promoting HCC cell migration and tumor development via β-catenin/HSF-1α/HSP70 pathway regulation. Thus, 14-3-3σ alone or combined with HSP70 are potential prognostic biomarkers for HCC

  10. Assessment of Analytic Water hammer Pressure Model of FAI/08-70

    Energy Technology Data Exchange (ETDEWEB)

    Park, Ju Yeop; Yoo, Seung Hun; Seul, Kwang-Won [Korea Institute of Nuclear Safety, Daejeon (Korea, Republic of)

    2016-10-15

    In evaluating water hammer effect on the safety related systems, methods developed by the US utility are likely to be adopted in Korea. For example, the US utility developed specific methods to evaluate pressure and loading transient on piping due to water hammer as in FAI/08-70. The methods of FAI/08-70 would be applied in Korea when any regulatory request on the evaluation of water hammer effect due to the non-condensable gas accumulation in the safety related systems. Specifically, FAI/08-70 gives an analytic model which can be used to analyze the maximum transient pressure and maximum transient loading on the piping of the safety-related systems due to the non-condensable induced water hammer effect. Therefore, it seems to be meaningful to review the FAI/08-70 methods and attempt to apply the methods to a specific case to see if they really give reasonable estimate before the application of FAI/08-70 methods to domestic nuclear power plants. In the present study, analytic water hammer pressure model of FAI/08-70 is reviewed in detail and the model is applied to the specific experiment of FAI/08-70 to see if the analytic water hammer pressure model really gives reasonable estimate of the peak water hammer pressure. Specifically, we assess the experiment 52A of FAI/08-70 which adopts flushed initial condition with a short rising piping length and a high level piping length of 51inch. The calculated analytic water hammer pressure peak shows a close agreement with the measured experimental data of 52A. Unfortunately, however, the theoretical value is a little bit less than that of the experimental value. This implies the analytic model of FAI/08-70 is not conservative.

  11. Micromechanical properties of C70 single crystals in the temperature range 77-350 K

    International Nuclear Information System (INIS)

    Lubenets, S.V.; Natsik, V.D.; Fomenko, L.S.; Rusakova, A.V.; Natsik, V.D.; Osip'yan, Yu.A.; Orlov, V.I.; Sidorov, N.S.; Izotov, A.N.

    2012-01-01

    Hexagonal single crystals of C 70 up to a size down to 1-2 mm were grown which allowed for the first time to investigate their low-temperature mechanical properties. Morphology, microplasticity anisotropy and the temperature dependence of Vickers microhardness HV (T) of the C 70 crystals in the temperature range 77-350 K involving all known phase transitions have been studied with the aid of optical microscopy and microindentation. The association of the features of HV (T) dependence with orientation phase transformations has been analyzed. It is suggested that anisotropy of microplasticity in the C 70 crystals correlates with the active slip systems.

  12. MCSA Windows Server 2012 R2 administration study guide exam 70-411

    CERN Document Server

    Panek, William

    2015-01-01

    Complete exam coverage, hands-on practice, and interactive studytools for the MCSA: Administering Windows Server 2012 R2 exam70-411 MCSA: Windows Server 2012 R2 Administration Study Guide: Exam70-411 provides comprehensive preparation for exam 70-411:Administering Windows Server 2012 R2. With full coverage of allexam domains, this guide contains everything you need to know to befully prepared on test day. Real-world scenarios illustrate thepractical applications of the lessons, and hands-on exercises allowyou to test yourself against everyday tasks. You get access to aninteractive practice te

  13. MCSA Windows Server 2012 R2 installation and configuration study guide exam 70-410

    CERN Document Server

    Panek, William

    2015-01-01

    Master Windows Server installation and configuration withhands-on practice and interactive study aids for the MCSA: WindowsServer 2012 R2 exam 70-410 MCSA: Windows Server 2012 R2 Installation and ConfigurationStudy Guide: Exam 70-410 provides complete preparationfor exam 70-410: Installing and Configuring Windows Server 2012 R2.With comprehensive coverage of all exam topics and plenty ofhands-on practice, this self-paced guide is the ideal resource forthose preparing for the MCSA on Windows Server 2012 R2. Real-worldscenarios demonstrate how the lessons are applied in everydaysettings. Reader

  14. Study of subshell gap around N = 70 for neutron-rich nuclei

    International Nuclear Information System (INIS)

    Hemalatha, M.

    2011-01-01

    The study and search for new regions of shell closure for nuclei away from stability is a topic of current interest both experimentally and theoretically. There have been few studies predicting a weak spherical subshell gap of 110 Zr (N = 70), for example. This is supported by a recent study indicating that the spherical N = 70 shell gap may not have a large effect at N = 68 for Zr isotopes. It would be, therefore, interesting to know whether there is a subshell closure at N = 70 in the neutron rich region and also for the very neutron-rich nuclei, 110 Zr

  15. Relationship between ash content and R{sub 70} self-heating rate of Callide Coal

    Energy Technology Data Exchange (ETDEWEB)

    Beamish, B. Basil; Blazak, Darren G. [School of Engineering, The University of Queensland, St Lucia, Qld 4072 (Australia)

    2005-10-17

    Borecore samples from the Trap Gully pit at Callide have been assessed using the R{sub 70} self-heating test. The highest R{sub 70} self-heating rate value was 16.22 {sup o}C/h, which is consistent with the subbituminous rank of the coal. R{sub 70} decreases significantly with increasing mineral matter content, as defined by the ash content of the coal. This effect is due to the mineral matter in the coal acting as a heat sink. A trendline equation has been fitted to the borecore data from the Trap Gully pit: R{sub 70}=0.0029xash{sup 2}-0.4889xash+20.644, where all parameters are on a dry-basis. This relationship can be used to model the self-heating hazard of the pit, both vertically and laterally. (author)

  16. Miniature 70-W Brushless Motor-Controller for Compact Extraterrestrial-Based Actuation, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — This SBIR will support rover locomotion and manipulation with a system of newly-developed penny-sized 70-W brushless servomotor controllers that are networked on a...

  17. Miniature 70-W Brushless Motor-Controller for Compact Extraterrestrial-Based Actuation, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — This SBIR will support rover locomotion and manipulation with a system of newly-developed penny-sized 70-W brushless servomotor controllers that are networked on a...

  18. Structure of a new crystal form of human Hsp70 ATPase domain.

    Science.gov (United States)

    Osipiuk, J; Walsh, M A; Freeman, B C; Morimoto, R I; Joachimiak, A

    1999-05-01

    Hsp70 proteins are highly conserved proteins induced by heat shock and other stress conditions. An ATP-binding domain of human Hsp70 protein has been crystallized in two major morphological forms at pH 7.0 in the presence of PEG 8000 and CaCl2. Both crystal forms belong to the orthorhombic space group P212121, but show no resemblance in unit-cell parameters. Analysis of the crystal structures for both forms shows a 1-2 A shift of one of the subdomains of the protein. This conformational change could reflect a 'natural' flexibility of the protein which might be relevant to ATP binding and may facilitate the interaction of other proteins with Hsp70 protein.

  19. 27 CFR 70.42 - Returns prepared or executed by appropriate TTB officers.

    Science.gov (United States)

    2010-04-01

    ...) of this section and subscribed by the appropriate TTB officer is prima facie good and sufficient for... PROCEDURE AND ADMINISTRATION Discovery of Liability and Enforcement of Laws General Powers and Duties § 70...

  20. Bayou Corne sinkhole : control measurements of State Highway 70 in Assumption Parish, Louisiana, tech summary.

    Science.gov (United States)

    2014-01-01

    The sinkhole located in Assumption Parish, Louisiana, threatens the stability of Highway 70, a state maintained route. In order to : mitigate the potential damaging e ects of the sinkhole on this infrastructure, the Louisiana Department of Transpo...

  1. Bayou Corne sinkhole : control measurements of State Highway 70 in Assumption Parish, Louisiana.

    Science.gov (United States)

    2014-01-01

    This project measured and assessed the surface stability of the portion of LA Highway 70 that is : potentially vulnerable to the Assumption Parish sinkhole. Using Global Positioning Systems (GPS) : enhanced by a real-time network (RTN) of continuousl...

  2. Bayou Corne Sinkhole: Control Measurements of State Highway 70 in Assumption Parish, Louisiana : Research Project Capsule

    Science.gov (United States)

    2012-09-01

    The sinkhole located in northern Assumption Parish, Louisiana, threatens : the stability of Highway 70, a state-maintained route. In order to monitor : and mitigate potential damage eff ects on this infrastructure, the Louisiana : Department of Trans...

  3. Hsp60 and p70S6K form a complex in human cardiomyocytes

    Directory of Open Access Journals (Sweden)

    Kroupskaya I. V.

    2011-02-01

    Full Text Available Molecular chaperon Hsp60 and protein kinase p70S6K play an important functional role in the regulation of cardiomyocytes vital function or apoptosis. Aim. To study a possibility of in vivo complex formation between Hsp60 and p70S6K in cardiomyocytes. Methods. Co-immunoprecipitation, Western-blot analysis. Results. We have identified in vivo interaction between molecular chaperone Hsp60 and two isoforms of proteinkinase p70S6K in human myocardium, normal and affected by cardiomyopathy. Conclusions. The results obtained suggest a possible participation of molecular chaperon Hsp60 in regulation of p70S6K activity in stressinduced apoptotic signaling pathway in cardiomyocytes.

  4. Photoinduced electron transfer to fullerene C70 (An in situ EPR study)

    International Nuclear Information System (INIS)

    Brezova, V.; Dvoranova, D.; Kostova, B.; Stasko, A.

    1999-01-01

    The photoexcitation of fullerene C 70 by monochromatic light (λ = 546 nm) in the presence of electron donating substrate 3,3',5,5'-tetramethyl-benzidine (TMB) resulted in the charge-transfer, quenching the fullerene triplet state and forming corresponding C 70 anion-radicals. Analogously to the photo-reduction of C 60 , two EPR signal were observed upon in situ irradiation in the cavity of EPR spectrometer. EPR singlet A characterized by g-value, g A = 2.0009 and peak-to-peak line-width, pp A = 0.013 mT was assigned to the C 70 mono-anion. Signal B (g B = 2.0011; pp B = 0.011 mT) was tentatively attributed to the C 70 di-anion or to the associated forms of mono-anions. The stabilization of photo-generated anion-radicals significantly depends on solvent polarity. (authors)

  5. 40 CFR 70.8 - Permit review by EPA and affected States.

    Science.gov (United States)

    2010-07-01

    ... compatible with EPA's national database management system. (2) The Administrator may waive the requirements... PROGRAMS (CONTINUED) STATE OPERATING PERMIT PROGRAMS § 70.8 Permit review by EPA and affected States. (a...

  6. Isospin-symmetry-breaking effects in A∼70 nuclei within beyond-mean-field approach

    Energy Technology Data Exchange (ETDEWEB)

    Petrovici, A.; Andrei, O. [National Institute for Physics and Nuclear Engineering, R-077125 Bucharest (Romania)

    2015-02-24

    Particular isospin-symmetry-breaking probes including Coulomb energy differences (CED), mirror energy differences (MED), and triplet energy differences (TED) manifest anomalies in the A∼70 isovector triplets of nuclei. The structure of proton-rich nuclei in the A∼70 mass region suggests shape coexistence and competition between pairing correlations in different channels. Recent results concerning the interplay between isospin-mixing and shape-coexistence effects on exotic phenomena in A∼70 nuclei obtained within the beyond-mean-field complex Excited Vampir variational model with symmetry projection before variation using a realistic effective interaction in a relatively large model space are presented. Excited Vampir predictions concerning the Gamow-Teller β decay to the odd-odd N=Z {sup 66}As and {sup 70}Br nuclei correlated with the pair structure analysis in the T=1 and T=0 channel of the involved wave functions are discussed.

  7. Hypoxia downregulates Ku70/80 expression in cervical carcinoma tumors

    International Nuclear Information System (INIS)

    Lara, Pedro Carlos; Lloret, Marta; Clavo, Bernardino; Apolinario, Rosa Maria; Bordon, Elisa; Rey, Agustin; Falcon, Orlando; Alonso, Ana Ruiz; Belka, Claus

    2008-01-01

    Hypoxia may inhibits the NHEJ DNA repair through downregulating Ku70/80 expression and combined with an increased angiogenesis and altered p53 expression would be responsible for tumor progression in cervical carcinoma

  8. 31 CFR 359.70 - May Public Debt waive any provision in this part?

    Science.gov (United States)

    2010-07-01

    ... UNITED STATES SAVINGS BONDS, SERIES I Miscellaneous Provisions § 359.70 May Public Debt waive any... unnecessary hardship: (a) If such action would not be inconsistent with law or equity; (b) If it does not...

  9. Real-time observation of the conformational dynamics of mitochondrial Hsp70 by spFRET.

    Science.gov (United States)

    Sikor, Martin; Mapa, Koyeli; von Voithenberg, Lena Voith; Mokranjac, Dejana; Lamb, Don C

    2013-05-29

    The numerous functions of the important class of molecular chaperones, heat shock proteins 70 (Hsp70), rely on cycles of intricate conformational changes driven by ATP-hydrolysis and regulated by cochaperones and substrates. Here, we used Förster resonance energy transfer to study the conformational dynamics of individual molecules of Ssc1, a mitochondrial Hsp70, in real time. The intrinsic dynamics of the substrate-binding domain of Ssc1 was observed to be uncoupled from the dynamic interactions between substrate- and nucleotide-binding domains. Analysis of the fluctuations in the interdomain separation revealed frequent transitions to a nucleotide-free state. The nucleotide-exchange factor Mge1 did not induce ADP release, as expected, but rather facilitated binding of ATP. These results indicate that the conformational cycle of Ssc1 is more elaborate than previously thought and provide insight into how the Hsp70s can perform a wide variety of functions.

  10. Hydrogen molecules inside fullerene C70: quantum dynamics, energetics, maximum occupancy, and comparison with C60.

    Science.gov (United States)

    Sebastianelli, Francesco; Xu, Minzhong; Bacić, Zlatko; Lawler, Ronald; Turro, Nicholas J

    2010-07-21

    Recent synthesis of the endohedral complexes of C(70) and its open-cage derivative with one and two H(2) molecules has opened the path for experimental and theoretical investigations of the unique dynamic, spectroscopic, and other properties of systems with multiple hydrogen molecules confined inside a nanoscale cavity. Here we report a rigorous theoretical study of the dynamics of the coupled translational and rotational motions of H(2) molecules in C(70) and C(60), which are highly quantum mechanical. Diffusion Monte Carlo (DMC) calculations were performed for up to three para-H(2) (p-H(2)) molecules encapsulated in C(70) and for one and two p-H(2) molecules inside C(60). These calculations provide a quantitative description of the ground-state properties, energetics, and the translation-rotation (T-R) zero-point energies (ZPEs) of the nanoconfined p-H(2) molecules and of the spatial distribution of two p-H(2) molecules in the cavity of C(70). The energy of the global minimum on the intermolecular potential energy surface (PES) is negative for one and two H(2) molecules in C(70) but has a high positive value when the third H(2) is added, implying that at most two H(2) molecules can be stabilized inside C(70). By the same criterion, in the case of C(60), only the endohedral complex with one H(2) molecule is energetically stable. Our results are consistent with the fact that recently both (H(2))(n)@C(70) (n = 1, 2) and H(2)@C(60) were prepared, but not (H(2))(3)@C(70) or (H(2))(2)@C(60). The ZPE of the coupled T-R motions, from the DMC calculations, grows rapidly with the number of caged p-H(2) molecules and is a significant fraction of the well depth of the intermolecular PES, 11% in the case of p-H(2)@C(70) and 52% for (p-H(2))(2)@C(70). Consequently, the T-R ZPE represents a major component of the energetics of the encapsulated H(2) molecules. The inclusion of the ZPE nearly doubles the energy by which (p-H(2))(3)@C(70) is destabilized and increases by 66% the

  11. Progranulin Recruits HSP70 to ?-Glucocerebrosidase and Is Therapeutic Against Gaucher Disease

    OpenAIRE

    Jian, Jinlong; Tian, Qing-Yun; Hettinghouse, Aubryanna; Zhao, Shuai; Liu, Helen; Wei, Jianlu; Grunig, Gabriele; Zhang, Wujuan; Setchell, Kenneth D.R.; Sun, Ying; Overkleeft, Herman S.; Chan, Gerald L.; Liu, Chuan-ju

    2016-01-01

    Highlights ? PGRN is required for lysosomal appearance of GCase and PGRN deficiency causes GCase/LIMP2 aggregation upon stress ? PGRN directly binds to GCase through a two-site mechanism ? PGRN recruits HSP70 to GCase and prevents GCase aggregation in response to stress ? PGRN derivative Pcgin binds to GCase and HSP70 and is therapeutic against Gaucher disease In this study, we demonstrate that PGRN directly binds to GCase and is required for the lysosomal appearance of GCase. In addition, HS...

  12. 27 CFR 70.146 - 45-day period for making disbursements.

    Science.gov (United States)

    2010-04-01

    ... tax lien filing, to the lien imposed by 26 U.S.C. 6321 if, and to the extent, a right to payment has... Collection of Excise and Special (Occupational) Tax Lien for Taxes § 70.146 45-day period for making disbursements. Even though a notice of a lien imposed by 26 U.S.C. 6321 is filed in accordance with § 70.149 of...

  13. 46 CFR 7.70 - Folly Island, SC to Hilton Head Island, SC.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Folly Island, SC to Hilton Head Island, SC. 7.70 Section... BOUNDARY LINES Atlantic Coast § 7.70 Folly Island, SC to Hilton Head Island, SC. (a) A line drawn from the...′ W. (Port Royal Sound Lighted Whistle Buoy “2PR”); thence to the easternmost extremity of Hilton Head...

  14. cDNA cloning and mRNA expression of heat shock protein 70 gene ...

    African Journals Online (AJOL)

    In this study, the full-length heat shock protein 70 of Tegillarca granosa was cloned from cDNA library by rapid amplification of cDNA end (RACE). The open reading frame (ORF) of heat shock protein 70 was 1968 bp, and it encoded a protein of 655 amino acids with a predicted molecular weight of 71.48 kDa and an ...

  15. GRAELLSIA 70 años después: la era digital

    Directory of Open Access Journals (Sweden)

    Martín Albaladejo, Carolina

    2014-12-01

    Full Text Available This article aims at commemorating the 70 years of existence of the journal Graellsia, recalling its inception, briefly describing its long career and welcoming its publication in digital format.En este artículo se pretende conmemorar los 70 años de existencia de la revista GRAELLSIA, evocando sus inicios, describiendo someramente su ya largo recorrido y dando la bienvenida a su publicación en formato digital.

  16. The installed nuclear capacity is expected to grow by 70% by 2030 according to WNA

    International Nuclear Information System (INIS)

    Anon

    2011-01-01

    The installed nuclear capacity is expected to grow by 70% in the next 20 years and to reach 614 GW in 2030 according to the World National Association (WNA). The main demand will come from China, India, Korea and the United-Kingdom. This growth will imply a 70% growth in the demand for uranium. Australia, Canada and Kazakhstan that own the biggest reserves of uranium will benefit from this growing demand. (A.C.)

  17. On the origin of extraordinary cyclic strengthening of the austenitic stainless steel Sanicro 25 during fatigue at 700 °C

    Czech Academy of Sciences Publication Activity Database

    Heczko, Milan; Esser, B.D.; Smith, T. M.; Beran, Přemysl; Mazánová, Veronika; Kruml, Tomáš; Polák, Jaroslav; Mills, M. J.

    2017-01-01

    Roč. 32, č. 23 (2017), s. 4342-4353 ISSN 0884-2914 R&D Projects: GA MŠk LM2015069; GA MŠk(CZ) LQ1601; GA ČR(CZ) GA13-23652S Institutional support: RVO:68081723 ; RVO:61389005 Keywords : steel * fatigue * scanning transmission electron microscopy (STEM) Subject RIV: JL - Materials Fatigue, Friction Mechanics; JL - Materials Fatigue, Friction Mechanics (UJF-V) OBOR OECD: Audio engineering, reliability analysis; Audio engineering, reliability analysis (UFM-A); Audio engineering, reliability analysis (UJF-V) Impact factor: 1.673, year: 2016

  18. Identification of an exported heat shock protein 70 in Plasmodium falciparum

    Directory of Open Access Journals (Sweden)

    Grover Manish

    2013-01-01

    Full Text Available Host cell remodelling is a hallmark of malaria pathogenesis. It involves protein folding, unfolding and trafficking events and thus participation of chaperones such as Hsp70s and Hsp40s is well speculated. Until recently, only Hsp40s were thought to be the sole representative of the parasite chaperones in the exportome. However, based on the re-annotated Plasmodium falciparum genome sequence, a putative candidate for exported Hsp70 has been reported, which otherwise was known to be a pseudogene. We raised a specific antiserum against a C-terminal peptide uniquely present in PfHsp70-x. Immunoblotting and immunofluorescence-based approaches in combination with sub-cellular fractionation by saponin and streptolysin-O have been taken to determine the expression and localization of PfHsp70-x in infected erythrocyte. The re-annotated sequence of PfHsp70-x reveals it to be a functional protein with an endoplasmic reticulum signal peptide. It gets maximally expressed at the schizont stage of intra-erythrocytic life cycle. Majority of the protein localizes to the parasitophorous vacuole and some of it gets exported to the erythrocyte compartment where it associates with Maurer’s clefts. The identification of an exported parasite Hsp70 chaperone presents us with the fact that the parasite has evolved customized chaperones which might be playing crucial roles in aspects of trafficking and host cell remodelling.

  19. The exported chaperone Hsp70-x supports virulence functions for Plasmodium falciparum blood stage parasites.

    Directory of Open Access Journals (Sweden)

    Sarah C Charnaud

    Full Text Available Malaria is caused by five different Plasmodium spp. in humans each of which modifies the host erythrocyte to survive and replicate. The two main causes of malaria, P. falciparum and P. vivax, differ in their ability to cause severe disease, mainly due to differences in the cytoadhesion of infected erythrocytes (IE in the microvasculature. Cytoadhesion of P. falciparum in the brain leads to a large number of deaths each year and is a consequence of exported parasite proteins, some of which modify the erythrocyte cytoskeleton while others such as PfEMP1 project onto the erythrocyte surface where they bind to endothelial cells. Here we investigate the effects of knocking out an exported Hsp70-type chaperone termed Hsp70-x that is present in P. falciparum but not P. vivax. Although the growth of Δhsp70-x parasites was unaffected, the export of PfEMP1 cytoadherence proteins was delayed and Δhsp70-x IE had reduced adhesion. The Δhsp70-x IE were also more rigid than wild-type controls indicating changes in the way the parasites modified their host erythrocyte. To investigate the cause of this, transcriptional and translational changes in exported and chaperone proteins were monitored and some changes were observed. We propose that PfHsp70-x is not essential for survival in vitro, but may be required for the efficient export and functioning of some P. falciparum exported proteins.

  20. The exported chaperone Hsp70-x supports virulence functions for Plasmodium falciparum blood stage parasites

    Science.gov (United States)

    Charnaud, Sarah C.; Dixon, Matthew W. A.; Nie, Catherine Q.; Chappell, Lia; Sanders, Paul R.; Nebl, Thomas; Hanssen, Eric; Berriman, Matthew; Chan, Jo-Anne; Blanch, Adam J.; Beeson, James G.; Rayner, Julian C.; Przyborski, Jude M.; Tilley, Leann; Crabb, Brendan S.

    2017-01-01

    Malaria is caused by five different Plasmodium spp. in humans each of which modifies the host erythrocyte to survive and replicate. The two main causes of malaria, P. falciparum and P. vivax, differ in their ability to cause severe disease, mainly due to differences in the cytoadhesion of infected erythrocytes (IE) in the microvasculature. Cytoadhesion of P. falciparum in the brain leads to a large number of deaths each year and is a consequence of exported parasite proteins, some of which modify the erythrocyte cytoskeleton while others such as PfEMP1 project onto the erythrocyte surface where they bind to endothelial cells. Here we investigate the effects of knocking out an exported Hsp70-type chaperone termed Hsp70-x that is present in P. falciparum but not P. vivax. Although the growth of Δhsp70-x parasites was unaffected, the export of PfEMP1 cytoadherence proteins was delayed and Δhsp70-x IE had reduced adhesion. The Δhsp70-x IE were also more rigid than wild-type controls indicating changes in the way the parasites modified their host erythrocyte. To investigate the cause of this, transcriptional and translational changes in exported and chaperone proteins were monitored and some changes were observed. We propose that PfHsp70-x is not essential for survival in vitro, but may be required for the efficient export and functioning of some P. falciparum exported proteins. PMID:28732045

  1. Morphological, structural and optical properties of MEH-PPV: PC70BM nanocomposite film

    Science.gov (United States)

    Mhamdi, Asya; Sweii, Fatma ben Slama; Saidi, Hamza; Saidi, Faouzi; Bouazizi, Abdelaziz

    2018-05-01

    In this report, the influence of annealing temperature and spin coating speed on the structural and morphological properties of a blend of poly (2-methoxy-5-(2-ethyl-oxy)-p-phenylene-vinylene) (MEH-PPV) and [6-6]-phenyl-C71-butyric acid methyl ester (PC70BM) layer has been investigated. The photoactive layer (MEH-PPV: PC70BM) was deposited on ZnO film deposited on top of indium tin oxide (ITO) substrate by spin-coating. The effect of spin coating speed via atomic force microscope (AFM) leads to conclude that high speed is favorable for a good homogeneity of the film surface and good aggregates dispersion. The optimized structure was studied by varying the annealing temperatures using X-ray diffraction (XRD). The XRD analysis indicates that annealing treatment promoted the ordered aggregation and crystallization of MEH-PPV: PC70BM films. Indeed, the blend ratio effect on the optical properties of MEH-PPV: PC70BM thin film was investigated. While, the effect of incorporation of PC70BM on the optical properties was studied using UV-Vis and photoluminescence (PL) measurement. We conclude that MEH-PPV: PC70BM (1:3) film leads to high charge transfer rate.

  2. Identification and Characterization of a Novel Human Methyltransferase Modulating Hsp70 Protein Function through Lysine Methylation*

    Science.gov (United States)

    Jakobsson, Magnus E.; Moen, Anders; Bousset, Luc; Egge-Jacobsen, Wolfgang; Kernstock, Stefan; Melki, Ronald; Falnes, Pål Ø.

    2013-01-01

    Hsp70 proteins constitute an evolutionarily conserved protein family of ATP-dependent molecular chaperones involved in a wide range of biological processes. Mammalian Hsp70 proteins are subject to various post-translational modifications, including methylation, but for most of these, a functional role has not been attributed. In this study, we identified the methyltransferase METTL21A as the enzyme responsible for trimethylation of a conserved lysine residue found in several human Hsp70 (HSPA) proteins. This enzyme, denoted by us as HSPA lysine (K) methyltransferase (HSPA-KMT), was found to catalyze trimethylation of various Hsp70 family members both in vitro and in vivo, and the reaction was stimulated by ATP. Furthermore, we show that HSPA-KMT exclusively methylates 70-kDa proteins in mammalian protein extracts, demonstrating that it is a highly specific enzyme. Finally, we show that trimethylation of HSPA8 (Hsc70) has functional consequences, as it alters the affinity of the chaperone for both the monomeric and fibrillar forms of the Parkinson disease-associated protein α-synuclein. PMID:23921388

  3. Hsc70 facilitates TGF-β-induced activation of Smad2/3 in fibroblastic NRK-49F cells

    Energy Technology Data Exchange (ETDEWEB)

    Ikezaki, Midori; Higashimoto, Natsuki; Matsumura, Ko; Ihara, Yoshito, E-mail: y-ihara@wakayama-med.ac.jp

    2016-08-26

    Heat-shock cognate protein 70 (Hsc70), a molecular chaperone constitutively expressed in the cell, is involved in the regulation of several cellular signaling pathways. In this study, we found that TGF-β-induced phosphorylation and nuclear translocation of Smad2/3 were suppressed in fibroblastic NRK-49F cells treated with small interfering RNA (siRNA) for Hsc70. In the cells underexpressing Hsc70, transcriptional induction of connective tissue growth factor (CTGF), a target gene of the TGF-β signaling, was also suppressed in the early phase of TGF-β stimulation. Upon stimulation with TGF-β, Hsc70 interacted with Smad2/3, suggesting functional interactions of Hsc70 and Smad2/3 for the activation of TGF-β-induced Smad signaling. Although the expression of heat-shock protein 70 (Hsp70) was upregulated in the cells treated with Hsc70 siRNA, TGF-β-induced Smad activation was not affected in the cells overexpressing Hsp70. Collectively, these results indicate that Hsc70, but not Hsp70, supportively regulates TGF-β-induced Smad signaling in NRK-49F cells. - Highlights: • Hsc70 siRNA treatment suppressed the expression of Hsc70 but induced the expression of Hsp70 in NRK-49F cells. • Hsc70 siRNA treatment suppressed the activation of Smad2/3 in the cells treated with TGF-β. • Hsc70 interacted with Smad2/3 on stimulation with TGF-β in the cells. • Hsp70 did not influence the TGF-β-induced activation of Smad2/3 in the cells overexpressing Hsp70.

  4. The role of ZAP70 kinase in acute lymphoblastic leukemia infiltration into the central nervous system.

    Science.gov (United States)

    Alsadeq, Ameera; Fedders, Henning; Vokuhl, Christian; Belau, Nele M; Zimmermann, Martin; Wirbelauer, Tim; Spielberg, Steffi; Vossen-Gajcy, Michaela; Cario, Gunnar; Schrappe, Martin; Schewe, Denis M

    2017-02-01

    Central nervous system infiltration and relapse are poorly understood in childhood acute lymphoblastic leukemia. We examined the role of zeta-chain-associated protein kinase 70 in preclinical models of central nervous system leukemia and performed correlative studies in patients. Zeta-chain-associated protein kinase 70 expression in acute lymphoblastic leukemia cells was modulated using short hairpin ribonucleic acid-mediated knockdown or ectopic expression. We show that zeta-chain-associated protein kinase 70 regulates CCR7/CXCR4 via activation of extracellular signal-regulated kinases. High expression of zeta-chain-associated protein kinase 70 in acute lymphoblastic leukemia cells resulted in a higher proportion of central nervous system leukemia in xenografts as compared to zeta-chain-associated protein kinase 70 low expressing counterparts. High zeta-chain-associated protein kinase 70 also enhanced the migration potential towards CCL19/CXCL12 gradients in vitro CCR7 blockade almost abrogated homing of acute lymphoblastic leukemia cells to the central nervous system in xenografts. In 130 B-cell precursor acute lymphoblastic leukemia and 117 T-cell acute lymphoblastic leukemia patients, zeta-chain-associated protein kinase 70 and CCR7/CXCR4 expression levels were significantly correlated. Zeta-chain-associated protein kinase 70 expression correlated with central nervous system disease in B-cell precursor acute lymphoblastic leukemia, and CCR7/CXCR4 correlated with central nervous system involvement in T-cell acute lymphoblastic leukemia patients. In multivariate analysis, zeta-chain-associated protein kinase 70 expression levels in the upper third and fourth quartiles were associated with central nervous system involvement in B-cell precursor acute lymphoblastic leukemia (odds ratio=7.48, 95% confidence interval, 2.06-27.17; odds ratio=6.86, 95% confidence interval, 1.86-25.26, respectively). CCR7 expression in the upper fourth quartile correlated with central

  5. Targeting Aberrant p70S6K Activation for Estrogen Receptor-Negative Breast Cancer Prevention.

    Science.gov (United States)

    Wang, Xiao; Yao, Jun; Wang, Jinyang; Zhang, Qingling; Brady, Samuel W; Arun, Banu; Seewaldt, Victoria L; Yu, Dihua

    2017-11-01

    The prevention of estrogen receptor-negative (ER-) breast cancer remains a major challenge in the cancer prevention field, although antiestrogen and aromatase inhibitors have shown adequate efficacy in preventing estrogen receptor-positive (ER + ) breast cancer. Lack of commonly expressed, druggable targets is a major obstacle for meeting this challenge. Previously, we detected the activation of Akt signaling pathway in atypical hyperplasic early-stage lesions of patients. In the current study, we found that Akt and the downstream 70 kDa ribosomal protein S6 kinase (p70S6K) signaling pathway was highly activated in ER - premalignant breast lesions and ER - breast cancer. In addition, p70S6K activation induced transformation of ER - human mammary epithelial cells (hMEC). Therefore, we explored the potential of targeting Akt/p70S6K in the p70S6K activated, ER - hMEC models and mouse mammary tumor models for the prevention of ER - breast cancer. We found that a clinically applicable Akt/p70S6K dual inhibitor, LY2780301, drastically decreased proliferation of hMECs with ErbB2-induced p70S6K activation via Cyclin B1 inhibition and cell-cycle blockade at G 0 -G 1 phase, while it did not significantly reverse the abnormal acinar morphology of these hMECs. In addition, a brief treatment of LY2780301 in MMTV- neu mice that developed atypical hyperplasia (ADH) and mammary intraepithelial neoplasia (MIN) lesions with activated p70S6K was sufficient to suppress S6 phosphorylation and decrease cell proliferation in hyperplasic MECs. In summary, targeting the aberrant Akt/p70S6K activation in ER - hMEC models in vitro and in the MMTV- neu transgenic mouse model in vivo effectively inhibited Akt/S6K signaling and reduced proliferation of hMECs in vitro and ADH/MIN lesions in vivo , indicating its potential in prevention of p70S6K activated ER - breast cancer. Cancer Prev Res; 10(11); 641-50. ©2017 AACR . ©2017 American Association for Cancer Research.

  6. Tumor associated CD70 expression is involved in promoting tumor migration and macrophage infiltration in GBM.

    Science.gov (United States)

    Ge, Haitao; Mu, Luyan; Jin, Linchun; Yang, Changlin; Chang, Yifan Emily; Long, Yu; DeLeon, Gabriel; Deleyrolle, Loic; Mitchell, Duane A; Kubilis, Paul S; Lu, Dunyue; Qi, Jiping; Gu, Yunhe; Lin, Zhiguo; Huang, Jianping

    2017-10-01

    Tumor migration/metastasis and immunosuppression are major obstacles in effective cancer therapy. Incidentally, these 2 hurdles usually coexist inside tumors, therefore making therapy significantly more complicated, as both oncogenic mechanisms must be addressed for successful therapeutic intervention. Our recent report highlights that the tumor expression of a TNF family member, CD70, is correlated with poor survival for primary gliomas. In this study, we investigated how CD70 expression by GBM affects the characteristics of tumor cells and the tumor microenvironment. We found that the ablation of CD70 in primary GBM decreased CD44 and SOX2 gene expression, and inhibited tumor migration, growth and the ability to attract monocyte-derived M2 macrophages in vitro. In the tumor microenvironment, CD70 was associated with immune cell infiltrates, such as T cells; myeloid-derived suppressor cells; and monocytes/macrophages based on the RNA-sequencing profile. The CD163+ macrophages were far more abundant than T cells were. This overwhelming level of macrophages was identified only in GBM and not in low-grade gliomas and normal brain specimens, implying their tumor association. CD70 was detected only on tumor cells, not on macrophages, and was highly correlated with CD163 gene expression in primary GBM. Additionally, the co-expression of the CD70 and CD163 genes was found to correlate with decreased survival for patients with primary GBM. Together, these data suggest that CD70 expression is involved in promoting tumor aggressiveness and immunosuppression via tumor-associated macrophage recruitment/activation. Our current efforts to target this molecule using chimeric antigen receptor T cells hold great potential for treating patients with GBM. © 2017 UICC.

  7. Hsc70 regulates cell surface ASIC2 expression and vascular smooth muscle cell migration.

    Science.gov (United States)

    Grifoni, Samira C; McKey, Susan E; Drummond, Heather A

    2008-05-01

    Recent studies suggest members of the degenerin (DEG)/epithelial Na(+) channel (ENaC)/acid-sensing ion channel (ASIC) protein family play an important role in vascular smooth muscle cell (VSMC) migration. In a previous investigation, we found suppression of a certain DEG/ENaC/ASIC member, ASIC2, increased VSMC chemotactic migration, raising the possibility that ASIC2 may play an inhibitory role. Because ASIC2 protein was retained in the cytoplasm, we reasoned increasing surface expression of ASIC2 might unmask the inhibitory role of ASIC2 in VSMC migration so we could test the hypothesis that ASIC2 inhibits VSMC migration. Therefore, we used the chemical chaperone glycerol to enhance ASIC2 expression. Glycerol 1) increased cytoplasm ASIC2 expression, 2) permitted detection of ASIC2 at the cell surface, and 3) inhibited platelet-derived growth factor (PDGF)-bb mediated VSMC migration. Furthermore, ASIC2 silencing completely abolished the inhibitory effect of glycerol on migration, suggesting upregulation of ASIC2 is responsible for glycerol-induced inhibition of VSMC migration. Because other investigators have shown that glycerol regulates ENaC/ASIC via interactions with a certain heat shock protein, heat shock protein 70 (Hsc70), we wanted to determine the importance of Hsc70 on ASIC2 expression in VSMCs. We found that Hsc70 silencing increases ASIC2 cell surface expression and inhibits VSMC migration, which is abolished by cosilencing ASIC2. These data demonstrate that Hsc70 inhibits ASIC2 expression, and, when the inhibitory effect of Hsc70 is removed, ASIC2 expression increases, resulting in reduced VSMC migration. Because VSMC migration contributes to vasculogenesis and remodeling following vascular injury, our findings raise the possibility that ASIC2-Hsc70 interactions may play a role in these processes.

  8. The Structural Basis for Activation and Inhibition of ZAP-70 Kinase Domain.

    Science.gov (United States)

    Huber, Roland G; Fan, Hao; Bond, Peter J

    2015-10-01

    ZAP-70 (Zeta-chain-associated protein kinase 70) is a tyrosine kinase that interacts directly with the activated T-cell receptor to transduce downstream signals, and is hence a major player in the regulation of the adaptive immune response. Dysfunction of ZAP-70 causes selective T cell deficiency that in turn results in persistent infections. ZAP-70 is activated by a variety of signals including phosphorylation of the kinase domain (KD), and binding of its regulatory tandem Src homology 2 (SH2) domains to the T cell receptor. The present study investigates molecular mechanisms of activation and inhibition of ZAP-70 via atomically detailed molecular dynamics simulation approaches. We report microsecond timescale simulations of five distinct states of the ZAP-70 KD, comprising apo, inhibited and three phosphorylated variants. Extensive analysis of local flexibility and correlated motions reveal crucial transitions between the states, thus elucidating crucial steps in the activation mechanism of the ZAP-70 KD. Furthermore, we rationalize previously observed staurosporine-bound crystal structures, suggesting that whilst the KD superficially resembles an "active-like" conformation, the inhibitor modulates the underlying protein dynamics and restricts it in a compact, rigid state inaccessible to ligands or cofactors. Finally, our analysis reveals a novel, potentially druggable pocket in close proximity to the activation loop of the kinase, and we subsequently use its structure in fragment-based virtual screening to develop a pharmacophore model. The pocket is distinct from classical type I or type II kinase pockets, and its discovery offers promise in future design of specific kinase inhibitors, whilst mutations in residues associated with this pocket are implicated in immunodeficiency in humans.

  9. The Structural Basis for Activation and Inhibition of ZAP-70 Kinase Domain.

    Directory of Open Access Journals (Sweden)

    Roland G Huber

    2015-10-01

    Full Text Available ZAP-70 (Zeta-chain-associated protein kinase 70 is a tyrosine kinase that interacts directly with the activated T-cell receptor to transduce downstream signals, and is hence a major player in the regulation of the adaptive immune response. Dysfunction of ZAP-70 causes selective T cell deficiency that in turn results in persistent infections. ZAP-70 is activated by a variety of signals including phosphorylation of the kinase domain (KD, and binding of its regulatory tandem Src homology 2 (SH2 domains to the T cell receptor. The present study investigates molecular mechanisms of activation and inhibition of ZAP-70 via atomically detailed molecular dynamics simulation approaches. We report microsecond timescale simulations of five distinct states of the ZAP-70 KD, comprising apo, inhibited and three phosphorylated variants. Extensive analysis of local flexibility and correlated motions reveal crucial transitions between the states, thus elucidating crucial steps in the activation mechanism of the ZAP-70 KD. Furthermore, we rationalize previously observed staurosporine-bound crystal structures, suggesting that whilst the KD superficially resembles an "active-like" conformation, the inhibitor modulates the underlying protein dynamics and restricts it in a compact, rigid state inaccessible to ligands or cofactors. Finally, our analysis reveals a novel, potentially druggable pocket in close proximity to the activation loop of the kinase, and we subsequently use its structure in fragment-based virtual screening to develop a pharmacophore model. The pocket is distinct from classical type I or type II kinase pockets, and its discovery offers promise in future design of specific kinase inhibitors, whilst mutations in residues associated with this pocket are implicated in immunodeficiency in humans.

  10. Expression of Ku70 predicts results of radiotherapy in prostate cancer

    Energy Technology Data Exchange (ETDEWEB)

    Hasegawa, Tomokazu; Someya, Masanori; Hori, Masakazu; Nakata, Kensei; Kitagawa, Mio; Tsuchiya, Takaaki; Sakata, Koh-ichi [Sapporo Medical University School of medicine, Department of Radiology, Chuo-ku, Sapporo, Hokkaido (Japan); Matsumoto, Yoshihisa [Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology, Meguro-ku, Tokyo (Japan); Nojima, Masanori [The University of Tokyo, The Institute of Medical Science Hospital, Minatoku, Tokyo (Japan); Masumori, Naoya [Sapporo Medical University School of medicine, Department of Urology, Chuo-ku, Sapporo, Hokkaido (Japan); Hasegawa, Tadashi [Sapporo Medical University School of medicine, Department of Surgical Pathology, Chuo-ku, Sapporo, Hokkaido (Japan)

    2017-01-15

    Therapeutic strategy for prostate cancer is decided according to T stage, Gleason score, and prostate-specific antigen (PSA) level. These clinical factors are not accurate enough to predict individual risk of local failure of prostate cancer after radiotherapy. Parameters involved with radiosensitivity are required to improve the predictive capability for local relapse. We analyzed 58 patients with localized adenocarcinoma of the prostate between August 2007 and October 2010 treated with 76 Gy of intensity-modulated radiotherapy (IMRT) as a discovery cohort and 42 patients between March 2001 and May 2007 treated with three-dimensional conformal radiotherapy (3D-CRT) as a validation cohort. Immunohistochemical examination for proteins involved in nonhomologous end-joining was performed using biopsy specimens. Ku70 expression was not correlated with various clinical parameters, such as the Gleason score and D'amico risk classification, indicating that Ku70 expression was an independent prognostic factor. The predictive value for PSA relapse was markedly improved after the combination of Gleason score and Ku70 expression, as compared with Gleason score alone. In patients treated with radiotherapy and androgen deprivation therapy (ADT), no relapses were observed in patients with Gleason score ≤7 or low Ku70 expression. In contrast, patients with Gleason score ≥8 and high Ku70 expression had high PSA relapse rates. In the validation cohort, similar results were obtained. Treatment with 76 Gy and ADT can be effective for patients with Gleason score ≤7 or low Ku70 expression, but is not enough for patients with Gleason score ≥8 and high Ku70 expression and, thus, require other treatment approaches. (orig.) [German] Die Behandlung beim Prostatakarzinom ist abhaengig von T-Stadium, Gleason-Score und prostataspezifischem Antigen (PSA). Diese klinischen Faktoren sind jedoch zu ungenau, um das individuelle Lokalrezidivrisiko beim Prostatakarzinom nach

  11. Cell-surface expression of Hsp70 on hematopoietic cancer cells after inhibition of HDAC activity

    DEFF Research Database (Denmark)

    Jensen, Helle; Andresen, Lars; Hansen, Karen Aagaard

    Heat shock proteins (HSPs) are highly conserved molecules, which support folding of proteins under physiological conditions and mediate protection against lethal damage after various stress stimuli. Five HSP families exist defined by their molecular size (i.e. HSP100, HSP90, HSP70, HSP60, and the......Heat shock proteins (HSPs) are highly conserved molecules, which support folding of proteins under physiological conditions and mediate protection against lethal damage after various stress stimuli. Five HSP families exist defined by their molecular size (i.e. HSP100, HSP90, HSP70, HSP60...... clinically applied reagents, such as alkyl-lysophospholipides, chemotherapeutic agents, and anti-inflammatory reagents, have been found to enhance Hsp70 surface expression on cancer cells. We have found that inhibition of histone deacetylase (HDAC) activity leads to surface expression of Hsp70 on various...... hematopoietic cancer cells, an occurance that was not observed on naïve or activated peripheral blood cells. HDAC-inhibitor mediated Hsp70 surface expression was confined to the apoptotic Annexin V positive cells and blocked by inhibition of apoptosis. Other chemotherapeutic inducers of apoptosis...

  12. THE 70 MONTH SWIFT-BAT ALL-SKY HARD X-RAY SURVEY

    International Nuclear Information System (INIS)

    Baumgartner, W. H.; Tueller, J.; Markwardt, C. B.; Skinner, G. K.; Barthelmy, S.; Gehrels, N.; Mushotzky, R. F.; Evans, P. A.

    2013-01-01

    We present the catalog of sources detected in 70 months of observations with the Burst Alert Telescope (BAT) hard X-ray detector on the Swift gamma-ray burst observatory. The Swift-BAT 70 month survey has detected 1171 hard X-ray sources (more than twice as many sources as the previous 22 month survey) in the 14-195 keV band down to a significance level of 4.8σ, associated with 1210 counterparts. The 70 month Swift-BAT survey is the most sensitive and uniform hard X-ray all-sky survey and reaches a flux level of 1.03 × 10 –11 erg s –1 cm –2 over 50% of the sky and 1.34 × 10 –11 erg s –1 cm –2 over 90% of the sky. The majority of new sources in the 70 month survey continue to be active galactic nuclei, with over 700 in the catalog. As part of this new edition of the Swift-BAT catalog, we also make available eight-channel spectra and monthly sampled light curves for each object detected in the survey in the online journal and at the Swift-BAT 70 month Web site

  13. Adenoviral transfer of HSP-70 into pulmonary epithelium ameliorates experimental acute respiratory distress syndrome.

    Science.gov (United States)

    Weiss, Yoram G; Maloyan, Alina; Tazelaar, John; Raj, Nichelle; Deutschman, Clifford S

    2002-09-01

    The acute respiratory distress syndrome (ARDS) provokes three pathologic processes: unchecked inflammation, interstitial/alveolar protein accumulation, and destruction of pulmonary epithelial cells. The highly conserved heat shock protein HSP-70 can limit all three responses but is not appropriately expressed in the lungs after cecal ligation and double puncture (2CLP), a clinically relevant model of ARDS. We hypothesize that restoring expression of HSP-70 using adenovirus-mediated gene therapy will limit pulmonary pathology following 2CLP. We administered a vector containing the porcine HSP-70 cDNA driven by a CMV promoter (AdHSP) into the lungs of rats subjected to 2CLP or sham operation. Administration of AdHSP after either sham operation or 2CLP increased HSP-70 protein expression in lung tissue, as determined by immunohistochemistry and Western blot hybridization. Administration of AdHSP significantly attenuated interstitial and alveolar edema and protein exudation and dramatically decreased neutrophil accumulation, relative to a control adenovirus. CLP-associated mortality at 48 hours was reduced by half. Modulation of HSP-70 production reduces pathologic changes and may improve outcome in experimental ARDS.

  14. THE 70 MONTH SWIFT-BAT ALL-SKY HARD X-RAY SURVEY

    Energy Technology Data Exchange (ETDEWEB)

    Baumgartner, W. H.; Tueller, J.; Markwardt, C. B.; Skinner, G. K.; Barthelmy, S.; Gehrels, N. [NASA/Goddard Space Flight Center, Astrophysics Science Division, Greenbelt, MD 20771 (United States); Mushotzky, R. F. [Department of Astronomy, University of Maryland, College Park, MD 20742 (United States); Evans, P. A., E-mail: whbaumga@alum.mit.edu [X-Ray and Observational Astronomy Group/Department of Physics and Astronomy, University of Leicester, Leicester, LE1 7RH (United Kingdom)

    2013-08-15

    We present the catalog of sources detected in 70 months of observations with the Burst Alert Telescope (BAT) hard X-ray detector on the Swift gamma-ray burst observatory. The Swift-BAT 70 month survey has detected 1171 hard X-ray sources (more than twice as many sources as the previous 22 month survey) in the 14-195 keV band down to a significance level of 4.8{sigma}, associated with 1210 counterparts. The 70 month Swift-BAT survey is the most sensitive and uniform hard X-ray all-sky survey and reaches a flux level of 1.03 Multiplication-Sign 10{sup -11} erg s{sup -1} cm{sup -2} over 50% of the sky and 1.34 Multiplication-Sign 10{sup -11} erg s{sup -1} cm{sup -2} over 90% of the sky. The majority of new sources in the 70 month survey continue to be active galactic nuclei, with over 700 in the catalog. As part of this new edition of the Swift-BAT catalog, we also make available eight-channel spectra and monthly sampled light curves for each object detected in the survey in the online journal and at the Swift-BAT 70 month Web site.

  15. Enhanced photocatalytic activity of BiOCl by C70 modification and mechanism insight

    Science.gov (United States)

    Ma, Dongmei; Zhong, Junbo; Li, Jianzhang; Wang, Li; Peng, Rufang

    2018-06-01

    As an excellent photocatalyst which can compete with TiO2, BiOCl has triggered increasing attention. However, the practical application of BiOCl has been significantly limited by the fast recombination of the photoinduced electron-hole charge pairs. In this study, to further enhance the separation efficiency of photoinduced electron-hole charge pairs of BiOCl, a series of efficient BiOCl photocatalysts were prepared by C70 surface modification. The trapping experiments reveal that the main active species were determined to be superoxide radicals (O2rad -) and holes (h+) under simulated sunlight irradiation. The surface photovoltage spectroscopy (SPS) demonstrates that separation of the photoinduced electron-hole pairs has been significantly promoted, forming more radOH, proven by terephthalic acid photoluminescence probing technique. The photocatalytic evaluation results display that the C70/BiOCl photocatalysts exhibit much higher photocatalytic activity in decolorization of rhodamine B (RhB) than that of the bare BiOCl under the simulated sunlight irradiation. The excellent electron acceptability of C70 is conducive to the separation of the photogenerated carriers and results in efficient formation of O2rad -, proven by the results of SPS and electron spin-resonance (ESR), therefore the photocatalytic performance of C70/BiOCl has been greatly improved. Based on all these observations, an enhancement mechanism in photocatalytic performance of C70/BiOCl was proposed.

  16. Expression profiling of Plasmodium berghei HSP70 genes for generation of bright red fluorescent parasites.

    Directory of Open Access Journals (Sweden)

    Marion Hliscs

    Full Text Available Live cell imaging of recombinant malarial parasites encoding fluorescent probes provides critical insights into parasite-host interactions and life cycle progression. In this study, we generated a red fluorescent line of the murine malarial parasite Plasmodium berghei. To allow constitutive and abundant expression of the mCherry protein we profiled expression of all members of the P. berghei heat shock protein 70 (HSP70 family. We identified PbHSP70/1, an invariant ortholog of Plasmodium falciparum HSP70-1, as the protein with the highest expression levels during Plasmodium blood, mosquito, and liver infection. Stable allelic insertion of a mCherry expression cassette into the PbHsp70/1 locus created constitutive red fluorescent P. berghei lines, termed Pbred. We show that these parasites can be used for live imaging of infected host cells and organs, including hepatocytes, erythrocytes, and whole Anopheles mosquitoes. Quantification of the fluorescence intensity of several Pbred parasite stages revealed significantly enhanced signal intensities in comparison to GFP expressed under the control of the constitutive EF1alpha promoter. We propose that systematic transcript profiling permits generation of reporter parasites, such as the Pbred lines described herein.

  17. Selection of unusual actinomycetal primary sigma70 factors by plant-colonizing Frankia strains.

    Science.gov (United States)

    Lavire, Céline; Blaha, Didier; Cournoyer, Benoit

    2004-02-01

    Functional adaptations of sigma70 transcriptional factors led to the emergence of several paralogous lineages, each one being specialized for gene transcription under particular growth conditions. Screening of a Frankia strain EaI-12 gene library by sigma70 DNA probing allowed the detection and characterization of a novel actinomycetal primary (housekeeping) sigma70 factor. Phylogenetic analysis positioned this factor in the RpoD cluster of proteobacterial and low-G+C-content gram-positive factors, a cluster previously free of any actinobacterial sequences. sigma70 DNA probing of Frankia total DNA blots and PCR screening detected one or two rpoD-like DNA regions per species. rpoD matched the conserved region in all of the species tested. The other region was found to contain sigA, an alternative primary factor. sigA appeared to be strictly distributed among Frankia species infecting plants by the root hair infection process. Both genes were transcribed by Frankia strain ACN14a grown in liquid cultures. The molecular phylogeny of the sigma70 family determined with Frankia sequences showed that the alternative actinomycetal factors and the essential ones belonged to the same radiation. At least seven distinct paralogous lineages were observed among this radiation, and gene transfers were detected in the HrdB actinomycetal lineage.

  18. IL-10 is significantly involved in HSP70-regulation of experimental subretinal fibrosis.

    Directory of Open Access Journals (Sweden)

    Yang Yang

    Full Text Available Subretinal fibrosis is directly related to severe visual loss, especially if occurs in the macula, and is frequently observed in advanced age-related macular degeneration and other refractory eye disorders such as diabetic retinopathy and uveitis. In this study, we analyzed the immunosuppressive mechanism of subretinal fibrosis using the novel animal model recently demonstrated. Both TLR2 and TLR4 deficient mice showed significant enlargement of subretinal fibrotic area as compared with wild-type mice. A single intraocular administration of heat shock protein 70 (HSP70, which is an endogenous ligand for TLR2 and TLR4, inhibited subretinal fibrosis in wild-type mice but not in TLR2 and TLR4-deficient mice. Additionally, HSP70 induced IL-10 production in eyes from wild-type mice but was impaired in both TLR2- and TLR4-deficient mice, indicating that HSP70-TLR2/TLR4 axis plays an immunomodulatory role in subretinal fibrosis. Thus, these results suggest that HSP70-TLR2/TLR4 axis is a new therapeutic target for subretinal fibrosis due to prognostic CNV.

  19. Proteomic analysis reveals heat shock protein 70 has a key role in polycythemia Vera.

    Science.gov (United States)

    Gallardo, Miguel; Barrio, Santiago; Fernandez, Marisol; Paradela, Alberto; Arenas, Alicia; Toldos, Oscar; Ayala, Rosa; Albizua, Enriqueta; Jimenez, Ana; Redondo, Santiago; Garcia-Martin, Rosa Maria; Gilsanz, Florinda; Albar, Juan Pablo; Martinez-Lopez, Joaquin

    2013-11-19

    JAK-STAT signaling through the JAK2V617F mutation is central to the pathogenesis of myeloproliferative neoplasms (MPN). However, other events could precede the JAK2 mutation. The aim of this study is to analyze the phenotypic divergence between polycytemia vera (PV) and essential thrombocytemia (ET) to find novel therapeutics targets by a proteomic and functional approach to identify alternative routes to JAK2 activation. Through 2D-DIGE and mass spectrometry of granulocyte protein from 20 MPN samples, showed differential expression of HSP70 in PV and ET besides other 60 proteins. Immunohistochemistry of 46 MPN bone marrow samples confirmed HSP70 expression. The median of positive granulocytes was 80% in PV (SD 35%) vs. 23% in ET (SD 34.25%). In an ex vivo model KNK437 was used as an inhibition model assay of HSP70, showed dose-dependent inhibition of cell growth and burst formation unit erythroid (BFU-E) in PV and ET, increased apoptosis in the erythroid lineage, and decreased pJAK2 signaling, as well as a specific siRNA for HSP70. These data suggest a key role for HSP70 in proliferation and survival of the erythroid lineage in PV, and may represent a potential therapeutic target in MPN, especially in PV.

  20. Type II shell evolution in A=70 isobars from the N≥40 island of inversion

    Directory of Open Access Journals (Sweden)

    A.I. Morales

    2017-02-01

    Full Text Available The level structures of 70Co and 70Ni, populated from the β decay of 70Fe, have been investigated using β-delayed γ-ray spectroscopy following in-flight fission of a 238U beam. The experimental results are compared to Monte-Carlo Shell-Model calculations including the pf+g9/2+d5/2 orbitals. The strong population of a (1+ state at 274 keV in 70Co is at variance with the expected excitation energy of ∼1 MeV from near spherical single-particle estimates. This observation indicates a dominance of prolate-deformed intruder configurations in the low-lying levels, which coexist with the normal near spherical states. It is shown that the β decay of the neutron-rich A=70 isobars from the new island of inversion to the Z=28 closed-shell regime progresses in accordance with a newly reported type of shell evolution, the so-called Type II, which involves many particle-hole excitations across energy gaps.